LOCUS       DAWEMX010000001        66699 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_211_length_66699_cov_13.458871, whole genome shotgun sequence.
ACCESSION   DAWEMX010000001 DAWEMX010000000
VERSION     DAWEMX010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 66699)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 66699)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..66699
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_66699_cov_13.458871"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3453..3462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7184..7193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23428..23437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    35718..35727
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        15335 a        20353 c        17530 g        13441 t
ORIGIN      
        1 acgggagcca aaatgatcca tgcggcggac gacactacgt ccaacatcgt ttccaaatcc
       61 atcagcatcg gcgaagggcg cgccagctac cggggccaag taatcatggg caaagggctt
      121 aaggggtgca ggaacaacac ggaatgcgac gctctacttc tggcagccaa cagccgtacg
      181 gacacctacc ctgccatcac ggtaaaaggg gatggaggca cggtgcagca tgaggcatcc
      241 gtctcccagg tctctgaaga aatgcttttt tacatgcagc agcggggcat cccgaaagct
      301 gcggcgatgt ctctggccgt caatggattc attaacgacc tcgttcagga attccccatg
      361 gaatactctg tggagctgcg caggctcatt gatctggaaa tggaagacag catcggataa
      421 ggagggaaat aatgaaccac ttgcttaacg aagaacaatt ttccgcaggc tttcttcccg
      481 catggtttga agcccggcgc cgtcatgccc tggaacaggc ggaacggctt ccggaacctt
      541 cccgccgcat ggaatcctgg cgtttcggca cccccggcaa cgaatccctg gaacatgctg
      601 aagcggccct ccctgtggcg ccggaagcct tggcccccat catcagggaa catgcctcca
      661 tgccggaggc cctccgcatt gtttacgcca acgggctccc cgtatccatc ccggaagagc
      721 ttccggaagg gctttccgtc atggatatgg aagactttgt gctccaatgc ccggacaccg
      781 cccggaaata tttggaaaca gagacggaaa cgctcggttc cgaaaaactg tcggcattga
      841 ctatggctct ccagcacaac gggctggtta ttatggcgga cagggaaatc tctctccctc
      901 tggaaatact ccacttcatt tccggagata acgtggccgt ttttccctct acgctgctca
      961 ttgcggaaac cggaagccgc ctgcacattc tggaacgcca ccttagcgca gaccatggcc
     1021 gccaattctg cggagcgctc caacaatacc acctttcctc cgcgtcatct gtcaaatacg
     1081 cgctggttca ggaacttaac agccgttccc gtgccgtgga gctttcccat attatggcgg
     1141 atgattccgc ggaaatggaa catctcgtca gccatcccgg cgcagcctgg gccagacagg
     1201 agacggtctg catcctgaac ggagacggcg caggagtccg cctgctttcc gccaaccatc
     1261 tgaaggaaaa caaggagctg gaccagagga cctgccagaa gcacctttac cgcggagcct
     1321 ccagcaacct gctttatgcc aatgtgcttg acgatgaagc cagcagcatt ttcagcggca
     1381 tgatcctggt ggcggaaggc gcgcatgaca ccagcgccta tcaaagcaac cgcaatctca
     1441 ttctcagccc cagagcggaa gccaactcca ttcccggcct ggaaatcctg gcggacagag
     1501 tgcagtgttc gcacggttcc gccacttcct ccatttcccc ggaagaaatt ttctacctgc
     1561 tttcacgggg cattccggaa cagacggcgc gcagaatgat tgcccagggc tttctgaaac
     1621 atgtcttgga ccaattcggc gatgaaacca tacgtaccgc ggtagaaagc atcatcctgc
     1681 cctgaccgct atttcatgaa acccaaaaag acggagagac ggtcccctat ccctcctttt
     1741 ccgtcctgat attaacgcgc cgctgcaaga gagccgccac aagcagaaac agagcagttc
     1801 cccccatcat gctgtaatac cattcacggt aaggcgtatg gtactgcagg aggatttccg
     1861 tctgctgttc cgaatccgga acagaaacaa gaatagaata gcgctcagta ctggataaag
     1921 gaagctcttc ccccttcccg gtgcgggcgc tccatccctt ggaatagctc tcaagaacag
     1981 acaattcctt tgttcctggc ggcacagtga catggatctt attattcgtt ctccaatccg
     2041 gaacaacagg agaaaaatga ttcttttccc cctgggttcg gacaaaatag cgtccacggt
     2101 acaagggatt ttccagcaag aagaaatctt ccgtttcctg aaccagattc cacccgtcaa
     2161 aagacggcct tccggctttg gcatcacaca acaaatgaga aatgccggcc atggcataat
     2221 ctttcggata ataatgtcca tccgacaaag gtctcagata tctgggctgc accgtttcat
     2281 aacctgaggc cagccttatt ccatatgtgg ataaatgatt agtacataaa aaatccaagt
     2341 cattatgagg attgacgata acaacggcac catcccccac atatccgcga aactcggaca
     2401 tccattccgg ttctttataa agatagccgc tttccggttt gtcggaatag gcaatccatg
     2461 aagaagaaaa cactatttgt tccccaaagg tacataatac cacaagtcct gcccaccact
     2521 tcggataacg gctggatata ttcaggcggg aacaggccca gccccctacg ctgaccagta
     2581 aaatcaataa ggcattgcgc caatcccaaa tcatcagctg gtccaggaga cgctcgcttc
     2641 tgagcaaata ccaggaatga cggtattttt gctgagaagt cgttatgttt ccgtcaataa
     2701 agctatgcaa ttttaacagc aatggttcat ggaaacactg aactgccacc gatacggcaa
     2761 gccatataca caaaattcca attccccacc atgcaagtct gcgccagata ggaagaaccg
     2821 gatgccgcct aaggtcaaat aactgccatc cggcaagcca gctcattcca aaagccatca
     2881 caaccgtgga gcgtgaatag aggaaagtgg aaagaggtgt gcatgaaaca attaaactga
     2941 caagaaaaca aaacttggcg gtcaacggtg cccggcggtt aaacaatgcc agaacagcca
     3001 gcacaaaaac aactcctcca ccaaatttga tatcgaataa atcagcgcca atcactttgg
     3061 aaatactgat tgtctgagga atgccgaaga gcgtcggcat cacagatgtg gccagaaaag
     3121 gaatattttc cagcccccag acaaacggca agcttttaca gccttccatg cgcggcaggg
     3181 tgtccacaaa aacatccaag gaaagcaacg ccgccaaaaa tccggagatc aaataaaaag
     3241 atgatgattt cagaaaaact ttccaggacc agcggtctgc ccgtttccac caatccgcaa
     3301 gaaacacgca cagcaccaga agaaaaacaa agagacacgc ttgcaaatgg cctcctctga
     3361 atccaagagc aataaagaga atagagggaa gatgcaccca tttgtttttc cttctccctt
     3421 ccaacaggga ccagacaatc aacggcgccc agnnnnnnnn nnatatccac ataataaact
     3481 gggaataaaa actgaaactc acagctccca gcaatacgta cggaatggga attcccctgg
     3541 atttcagtag aatgatcatc cccagccctg cgataaaaaa ctgcagcaag attcccgcat
     3601 cccatgccgt ccagaaagga agaaaaccaa ataaccaatg atgccaatcc cccgggctta
     3661 acatggtatt ttccggcagt gaagtcccat tgtgagtata agggttccat cccatatacc
     3721 catcactttg ccaggactgc tgcatcgccc agctataagg taaatattga gaagccccat
     3781 ctacgttata ttggttatgg acttcctcca ttggctgagt tgcgaatggt ttgaacaggc
     3841 attccacaca atccaccggg gctaaaacct ttcctccaaa cacggcagga gcaaaaaaaa
     3901 gcagtacaag tactgcccaa attattatcc cccaccacaa gggaattttt ctattttttt
     3961 tacaattcat ttataataaa aatctgacat tggctcctca tgcatcccca aagaaaattc
     4021 ctgcaaatta acgaatgaat ttctccacag aattgttaaa cccggcatcc atcgttctcc
     4081 ttcaactctg aaattaaacc aatgaatcgg aacgcctttt tctagcggat ttacaatccg
     4141 ttacggacag taaaagaaaa aaactcttgt tctcagggaa agtgccgctc tttatacggt
     4201 tattgaaacg tgactccgct tcaacatgca acgaactcta taaaagaggg tttgccaaaa
     4261 ttctacttca ggcccggata tcgtagcaat ttcccgtatt cccgtcacca gggtgggccc
     4321 aggcaaattt ctgtgctacg taagaggccc tgatacgtaa agaaacgatg ttgcaagttc
     4381 ttttctctta gagttacagc ttgactccgg attgtaaatc cgcccacctt ctcaagaaag
     4441 cactatttca ttgaaaaata cttcacccag aattattttc atcctgaatt cccagttaag
     4501 catcttaaga aaagcatcaa aaaatctggc cggaatgata tgaaatagcc ttgcagccgg
     4561 tttatgacct gtccgacaag caaattgcta ccacaaatat ttatgattca tcattccata
     4621 taaaaatata aataaaaagg aaccggacaa acaggaacgg ttcggataaa taagtagtcc
     4681 gaaacaaaag gaaaccgaaa ggctcaccct gtgagcatct catccggcat ccggtaagga
     4741 aaacgaaact attttttgaa acaaggaaga gtgtttctcc gttattatta ccgtatggtc
     4801 acattttaca acatcactct ctctaccgcc ttttccctgc tgatgctgtc cgtggcggaa
     4861 gcggcacggc ccaatgtggt tctcatcaat gcggatgacc ttggctgggc ggaagtaggc
     4921 tgctacggtc agaaaaaaat taaaaccccg aacattgaca agctggcctc cgaaggacag
     4981 cgatgggttt atttctattc cggagctccg gtttgttccc cttcccgcaa tgtgctgatg
     5041 acgggcaagc atacgggcaa ctgcgacgta caggatttga aacgcgtgga cgcgggcgaa
     5101 aactggcgcg acctcaaagg agactggccc atcagaacgg aaacctacac tctaccggaa
     5161 gccatgaaaa aagccggtta cgccacagcg gtgttcggta aatggggtat tggggatttc
     5221 ggttccaccg gagcgccgga caaacacggc gtggacaggt tctatggcta cacggaccag
     5281 aaagcctgcc acacctacta tcctccatac ctctggaatg acggaaagaa ggaagttctc
     5341 aacacttctc tgacagccgc cactatcgga cacggttccc agcccaaagg ggaagttctg
     5401 gcggacacct accgcgcgga acaacacagt tccgatctta ttgcggataa aatgctggaa
     5461 tttgtgaagg aaaaggccca tggcaaacaa ccgtttttcc tgtattacgc cccgctggaa
     5521 ccccatgtgg ccatgcagcc tcttcaggaa tggattgacc gctatccccg cgaatgggat
     5581 aaatccccct accgcggcaa ccggggctat ctgccccatc cccgcccccg ggccgcctat
     5641 gcaggcatga tttcccagat ggaccacaac gtaggacgcc tgctggacac gctgaaagcc
     5701 tgtggcctgg acaaaaatac catcgtcatt tttaccagcg acaacggcac cacgcatgat
     5761 gcaggggggg tagaccaccg gttcttcaac tccgtagccg atctcaaagg tttgaaaggg
     5821 cagctttatg aaggcggtat acgtgtcccc ggcattatcc gctggcctgg gaaaatagcc
     5881 ccgggaaaaa ccatcaccca gccggccttc catgcggacg tgatgcctac actgtgcgct
     5941 ctgacaggag cggatgcagg ttctccgctg ggaacggacc tctcccctgt ccttctgggc
     6001 aaaaaatccg ctctgcatga caggaagccc ctggtctggg cagggggagg ctacggcggc
     6061 caggtagccg tgcgtttcaa ctccaagaaa gtcatccgcc gcaacctgtt tcccggtaaa
     6121 aaaccggaca actgggaagt gtacgatatc gtgaaagacc ccgcagagaa aaataatatc
     6181 gccgcagaaa accgtgacct tatcaacaga gccatcgcca ttctggacag ggaatatcaa
     6241 cccgcgcccg gcttccaggc cctgcgttac aaggccccgg aacaggtagc caaataagag
     6301 aacgggaaag aaggttccac gatccgcctc cttcgccatt ctgaccttga cgtgaacgga
     6361 gccggggtta gtctttcacg catgagtttg acatcatccc tcctttccag ggtgctcgga
     6421 ggcggttcct gctccagaga agaattgata gccctcagcc gggaaccgct ggaagaattg
     6481 tgccaggcgg ccaacgccat ccgggaacat ttttgcggga atgtcttcga cctctgcacc
     6541 atcatcaacg gccgcagcgg caaatgttcg gaaaactgca agtactgcgc ccagtccgcc
     6601 cattactcca cggcagtgga ggaatatccc cttttaagtg atgaagccct cctggcgggg
     6661 gccaggtaca atgacgaacg tggcattctg cgctactcca tcgtcacctc cggcaagagg
     6721 ctgacggatg aagacgtgga ccggctctgc accagttacc ggcacatcgc cgaacattgc
     6781 ggcatctccc tctgcgcctc ccacggcctt atttccaaga aacactgcga gcaattgaaa
     6841 gccgcaggcg tttcacgcta ccacaataat ctggaaactt cccgccgcaa tttccctaat
     6901 gtctgcacca cccatacgta cgacgacaaa ctgcaaacca tcaaatgggc tctggaagcc
     6961 gggctggaag tttgcagcgg cggcattatg gggcttggag agactatgga agaccggata
     7021 gacatgtaca tggacatcgc ggcgctgggc atcaaatcca tgcccgtcaa ctttctgacc
     7081 cccatccccg gaacgccgta cgcggacatg acccccctgg gagaggaaga acagctgcgt
     7141 atcgtggccc tggtgcggtt catcatgccg gacggcttcg tccnnnnnnn nnnaaggacc
     7201 acggcaggaa aatcttcatg tccggagcga atgccgccat ctccggagat atgcttacta
     7261 ccgccggcgt caccatccgg gaagacctag ccatgctggc ggaactggga tatgaagtcc
     7321 gcatgaaata aggtccgtcc cattttcgcg ccctttgcca tgaatcactc cgactggtca
     7381 aaaaaagatc tggaatacat ctggcatccc tgttcccaga tgaaggatta cgaaacgctg
     7441 cctcctatcg tggtcgacca cggaaaaggc gtcaacctgt atgatgtgga cgggaaacgc
     7501 tatctggatg tggtcagctc ctggtggtgc aacctgctgg gccattccca ccccaaaatc
     7561 aaccgtgcca tcaaggaaca gcttgattcc ctggagcatg tcatcttcgc caacttctca
     7621 cacaggccgg ctatcactct gtgtgaagaa ctgatgaaga aaattccgcg ggggctctgc
     7681 aaattcaatt tctcggataa cggttcggca tccatcgaat ccgccatgaa aatgagtttc
     7741 caataccact accagaccgg aaaccgccag aaagtacgtt tcatgtccct gagcgacgcc
     7801 taccacggag aaaccctggg agccctctcc gcaggaggcc tggaccttta ttcggaactc
     7861 tacaaacctc ttctgttgga catcgtgcgc attcccgctc cggattgcta ccgctgcccc
     7921 aaggggaaca aacgcgactg ctgccaggcg gaatgcattg acgcggcaca ggaggccttt
     7981 gcgcgccatg gggacgaatg cgccgccctg ctggtggaac ccctgctcca gggctccgca
     8041 ggcatgcgga tgtatcctcc cgcttatctg gcgaaactac gttccctgtg cgacgcctac
     8101 aacgtgcacc tcattgcgga tgaaatcgcc accggttttg gccgcacggg gagcatgttc
     8161 gcctgcgacc aggccggcgt ttccccggac atcatgtgcg tctccaaagg gctcacgggc
     8221 ggctatatgc ccatgtccat caccatcacc acgcagaaaa tttacgatgc cttttatgcg
     8281 gactacaggg aaggaaaagc attcatgcac agccatacct attccggcaa tccgctgggg
     8341 tgctccgcag ctctcgccgt gctgaaggtc ctggacgagg aacacattat tccccgggcg
     8401 caggaaaagg cccctctttt ccgccagatg atcgtggagg ccctggggga tcatccccac
     8461 gtgggggaaa tacgcagcct gggcctagtg aacgccattg aactggtgga gaacagggaa
     8521 accaaaaaaa gctttccgtc cgagcagagg ctggggtacc ggatttacag ggaaaccctt
     8581 aaacagggac ttctgcttcg ccccctgggc gacgtcctgt atttcaatcc tcccctcgtc
     8641 atttctccgg aggaaatgga ggaagccgta tccatctgcg cttcctgcat ccgcaaagtg
     8701 ctgggataaa tgttcatccg gcacaacaag acgaactgtt ttttcttgaa gaagattgtg
     8761 aaccaccaat atagtgcaat tataatttga caaaagccgg gatgcggcag ctacaataca
     8821 gccaccgcgc cccgtttttt tatgagtgca gataccaaaa tgttacgcaa tagaacggca
     8881 ttttttttgg gggggtaaag gtataaaacc ttattgtctt atttccccgc ccgggaagcc
     8941 cgcataaatc aagttttttt ctcccctatc gcgtcacatt cgcccgcttt ttcctcatgt
     9001 gctcggcgtt atgtatgcgg gtgatcccgt tgtaagcact tttgaatttt gaagcatggt
     9061 atcaacagga gctgcaaata caatatttcc gcaatgaatt tgacaataaa ggagaaatgc
     9121 ttatggaacc agtcaatttg ttgaaagcta aaaatcgtga tgagttaagg caatggcttg
     9181 aacagaatca taagactgaa aaagaatgct gggttgttgt aaaacgtggt aaaccaaaag
     9241 ggaataacac attttggtat attgacgcag tagaagaagc tatgtgtttc ggctggattg
     9301 atagtacaac aaaaaaactc gataatggta tcactgcaca aaaactggct ccccgcagaa
     9361 cagtcagctt atggtctgaa ctaaataagg aacgctgccg caggatggaa aaattaggaa
     9421 aaatgacgga agccggacgt tctgctttac ccgatatgtc atcagcaggt tttatcatag
     9481 ataaagaaat tctaaaagca ttacaaactg ataacgaagt gtgggaaaat tttcttaaat
     9541 ttccacctct ttatcagagg gtacgcatag atactataca gataaaaaag aaacaaccac
     9601 aactttttca aagaagatta caaaagttgc ttgagaatac aaaaaaagga ataatgtacg
     9661 gcgaatggaa tgataatggg cgattattag actattagct ccggcaacca cgttttttaa
     9721 gacaaaagat gcaacttttc ttcaggaccg gagtttgtaa agataccttg caatctcata
     9781 ccaccatgat acgcctcacc ccgctccgca aacgtccggc gctttccgcg ctggtgcttc
     9841 tcctcctggg atccggcttc tgccaagctc aggaaatgct taccgattcc gcaagaaagg
     9901 aagcttccga atggcttaag gaagtcaaag ccggctacct gaacaccagc tcttcccgta
     9961 tccagaaggc cgtggccgcc cttaccgcgg ccaccagcac ggaaaacgcc gccatgaaac
    10021 tttacgtgga tgccatgaag gaccggtttc tgaaccccac cagcatgatg tcccgcatgc
    10081 tgaaccgggg aggcgggggc ggcttccgca tgatgcgcat gccgggttcc ggagggaggg
    10141 gaggcaacag cagcaagcct gacagcccca gcaccgcctt ttccaactgg cgcaagcaga
    10201 atacgggaaa caacgtagct cccggcttta aaaaagccct tcaaatccag tgcaaatgga
    10261 tgctgctttg cctgagaaaa gcggacgcgg aaaaaaacga acgcgacctt aacgtttcct
    10321 ccagcgtcct gtccatgctg gatgaagtgg ccgccaacgc caaggatgta ggcgaacagc
    10381 ttcccatggt aagcggagcc agcgaagtca tccgcaccta cctgaacatt ggagactacc
    10441 gttcggaaac cctgccggac aacctgatgg atctcaatac catcttcgac cgcgtactgc
    10501 tggctcccta caaggagaaa aaggatgtgg aaaacttccg gaaactctgg aacaaacgca
    10561 tcggcctgga agcgactctg ctgcaaaaca actccgccag cgataaaaaa acagcggaag
    10621 cagaaaaagc cgatttcctg gttaaaagac agtgggaacg ggaaaaggcc tgctttgaac
    10681 tcggcgacca ggtagcggca cttgaaaaga tgaaaagcct gatttccggc atcaaggacc
    10741 cttctgaaaa gcagcgtgcc atccgcgacc tggaattcct gcttcttacc ccggcggaac
    10801 aggcgaaact ccgcgaagag cgcatgaaca aacgcatgcc gggcggcccg cgcttttaac
    10861 aatcgtcgcc aagccccagc ctccctcctt tcatcccccg ttgcgtaacg cgcatcgggg
    10921 gattttacat tgcggagagc cgcatttccc tgacggaggc agcttccttc ctgccgggtt
    10981 ggaaccatgt cttgcgcagg cggtaaacgc gcacgccctg gatattctca tccagatgcc
    11041 aggctgtgcg aacgccgtgc tgctcccaga tatggcggct gagcatcccc atgaacttgc
    11101 tgtccatatc ggaaacgtca ccttgcggag tgagacagac aatgtacaca ttatccctgc
    11161 acaggttcag ggaggactgc cccacccacc ccatctcctt cagtttccgc tgtacgggtt
    11221 tcaacggaaa attccagccg cccagcgtga caacgttctt ccgggaacag ctccaggcct
    11281 tgcggaaaac agactgcccc ggggccagat cgtcaatagt gccgccggac atctggacgc
    11341 aaaacaggga atcgggctcc cggtccaccc gctcgcataa caggcgcccg gcatccgggg
    11401 aaaaagactc cagattagcc tcccacacgg cggcgcacgc ctgggacaaa gaaacggaga
    11461 acgcgcagaa catgggcacg ccgcccgcaa gagctccctt ccccgtccag gtgatgcgga
    11521 tgggacgata tttatccgac cacagcagac acagccctgc aatcaggaac gttggataaa
    11581 ctacgcggaa atacaggcgg tctatgctgg ccatatacag cagaatcagc accagcaggg
    11641 agcctacgca gcacacgagg gccggagaat ccccgcgccg gaaagaaacg cggcatgcca
    11701 gaccgaacaa aatccagcag gggagaagat ccataacgga atcccatgaa aacagatgca
    11761 aggctttctc tccacgggcc cacgccctgg gccagaattc acctccgtcc cgcgccagca
    11821 gaagcctttc cagataatcc gtattgaata catccatatc gcaattggta aacatccgga
    11881 ccatcctgat atcgttggcg aacacgccgt acttttcaca ctggtaggcg ctctcctgcc
    11941 tgatgggaca cgtgtccgag agaagggcgc gggcctggtt ccatgccgcc acgtcacatc
    12001 cctccagcac attccggtgg gcgtatttat tatattcatt cagacagaag gacgcaatga
    12061 gcacacaggc cagagccagc cagagacgac tgagggcaaa cagcttttcc ctggacggcc
    12121 tgagcccttc cagactgcct gccagcagga tggcccccca gaaagcgtcg cacaccaggg
    12181 cggcatccag ccggaccatg ctgcccagca cgcacaggaa tactcccgcg caccaccccc
    12241 tccagaaaga gccgtacttc aaacacgcca gcacacctgt aaatcctgtc aaaaaggcgg
    12301 catggctgaa ttgcagagcg gcgtaaaagc ccggcatgat cagaagccac accagcagca
    12361 gaaccagcgt attgaaatca tatctgcgcg ccttgcgggc atgcgccgtc agcatcgtga
    12421 aaatggccgc accgctggac aacgtggcaa gcgccagaaa cacaggccag acggacaccc
    12481 cgccaagggc aggcgcagct ttcaacagaa gcccggtcaa caggggattg acgaaaatga
    12541 catgggcgtc cgcataagag ggatcccccc atccgtcaag cacggacgca atcaccagat
    12601 cgtcattgga ctggtacgcc acgcccacaa ggctccaggc cagaaccaga agcaacaggt
    12661 tgaaccatac gggatggagc agaggaaaaa gcgataccca tctccaaatc tgtttcaaca
    12721 taagcaaatt ccctgacttt acagactgtt ttccctgtcc ggggacgcat actaggaacg
    12781 gggaaaatga aagtcaagag ccgatccgga tgaacaaacc ggaggggcta aaaggctgca
    12841 acctcttcct taacggttca ggcagacgat aatgcccgcg ataatcatcg cgttgccgcc
    12901 cactttgcgc atggtgatcg gttttccgaa aaaaatccgg tcaaacaaaa ggatatatac
    12961 atatgcggag cattccagca tgttcaccgt aatcatcggc aggtgggaca ggcagtaaaa
    13021 cgtcagcagg cagcagccca aaaaaacgga atatgccgcc atcgtggccg gattcacgaa
    13081 ataggcaagg ccggtcagcc ggtcattcat ggcggacttt ttcaaaacca cctgtgtgca
    13141 ggaagcaaga aaaacggaaa acaggttcag cccaatataa aattcttcac tcatgcgcct
    13201 cctccatgga aaccaaaata atacccccga tgaccagcgc cgctcccgcc gccagcgtcc
    13261 aggaaagagc ctcccccagg caaagccatg ccagcaggat accccagatg acaacggcgg
    13321 aacggttggc ataagccaca cccaggttga attttttcag tatgacctgc catcccgccg
    13381 cataaatgcc caacaggaac agcacgccgg ccagcagggc gaaccggcgc cagtccgtcc
    13441 atccgtaggg ggcagccatg cgccccagca cggtattcag cgcgtaaacg ataaacagca
    13501 ggtgcagaat caggtagttg atcatgaaag aaataaagag ccgggaacaa ttcactcata
    13561 ccggcgcgcc accactacgg cgggcctgtg cttggtttcc ttgtaaatct tggaaatgta
    13621 aatgccgata acgcccaggc tcatcaaaat caagcctccg accagccaga tggaggccac
    13681 ggaagaggcc cagccgggag gggctacatt atacagaagc tggcgcacaa ccacccaggc
    13741 gacatacagc atggctacca gggtgaccgc caggccaaag taggaaatat acaccagcgg
    13801 cctggcgctg taggaaacca cggaattcac cgccagcgcc atttttttgc ggaaagtgta
    13861 ggtcgttgga gaagaggatg ctttcttcac cctgcacggc acctgccgga aaccggcgga
    13921 aacacacagc ccaaagaaaa aaggctcccg gtcctggaat ttcagcatct cccgcaggaa
    13981 acgcgcgctc atcagccgcg ccgtaaccat attcctggta atctgctggt cggaaagcaa
    14041 attaaacaat gtgtagaaca attctccgga aacggcttca aacaagccgc ccttcctctt
    14101 ctcctgcacc ccgtacacca catccgcccc ggtagaatcc atctcacggg aaaaatcaac
    14161 cagccactcg ggagattcct ccaaatcact gtcaatcaag aatacgcgct ctccccgggc
    14221 ttcttcgcag gccacgtaca tggccagatg atggccgaag ttgcgggata aatccaccac
    14281 gcgcacccgg ccgtccttct cccgctcctt caacgcctgt tccagaccgt cccttgggca
    14341 gccgtcattc accagcacca cctccgtgcg ggaggggtca taacccgcct caagcgcgac
    14401 ggcgaaactg cgggaacaga actccgccag ataaccgccc gtacaataca cggtggccac
    14461 aatggacaat tgaacttgtg gatcagtcat aacgctttta catacatgag aacgcgcttc
    14521 ctttccccgc ttttcacgca cacctcgtcc ttttccggaa cacgggcaaa acccatgcgc
    14581 tcatgaaaac gcacggaagc cgcattgtcc tccagaatcc gggcatataa ccgttccaga
    14641 cccagctccc ctgcggccag ctccatcatc ccggcatatg cagcgaaagc cgggcgcagt
    14701 tccctgaatg ccctgtcgta aagataaatg cctatctctc cgcaacgcgt attccggtca
    14761 aaccagggca ggtaaaccag acccagcgga acctggtccg cataaatcac gcaggcgcgg
    14821 gcatcctccc cgcggacgtt cctttccagc cacgcccggt gctgctcctc cgttacctgg
    14881 tccaacaaaa aaaacgggct gaccttttcg gaattgcgcc acacgcgcaa gcggctgcgc
    14941 atggcctcat ccacaccaga cacgttgacc aggcaaattt tcatcatgat tcaactccct
    15001 ccagcccgaa gcctgcccgc aacacctccc ggcagccttc ttcccccaaa tcgtaataca
    15061 aaggaagcct cagcaacgta tcgctggtgc ggtccgtatg aggcatggcc ccggcagaac
    15121 gcccgaatct catcccggca ggggaggaat ggaggggaat gtagtggaac accgctttaa
    15181 tcccgtgctc cgccagcttg ttgatcaagc gcgtgcgctc ctccatggaa cggagcagca
    15241 cgtaatacat gtgggcgtta tgcgtgcaat gctccggaac gcacggacgc ctcagccccc
    15301 cctgttcttc caatggcgcc agagcgcggt ggtacatatt ccaccacttc agcctttccc
    15361 cgttgatctc ctgcccgtgt tccagctggg catacaaata agcggcggtc atctctccgg
    15421 gcagatagga ggaacccacg tccacccagg tgtatttgtc cacctgcccg cggaaaaacc
    15481 tggcacggtt ggtccctttt tcccggataa tctccgcgcg ttccatcagg gaggggtcat
    15541 tcaccagcag ggctcctccc tctccggaaa tgacattctt ggtctcgtgg aaactgtaac
    15601 agcccaaatg gccgatggag ccgagccgac gccctctgta gcaggagccg acgccctggg
    15661 cggcgtcttc aatcacatac aggcgatgcc tcccggcaat ttccatgatt gcatccattt
    15721 cacaagccac gcctgcgtaa tgaaccacgc aaatggcgcg ggtgcggtcc gtgatggccg
    15781 cttctatcag cgtctcgtca atattctgcg tatccggcct gatatccacg aatacaggaa
    15841 cgccgccgcg cagcacaaac gcgttcgccg tggagacgaa cgtataggaa ggcatgataa
    15901 tctcgtcgcc cgggcggcag tcgatcaaca gggccgccat ctccagcgcg gcggttccgg
    15961 aatgaaccag cagaaccttt ctggcgccgg tgcgttccgc aagcagttca tggcattttt
    16021 tcgtgtaata gccgtctccg cataaatggg cgcgctggat ggcgtcccgg atataatcca
    16081 attcgggacc gtgctggtga ggttggttga aaggaatctc catggcttca gaaaagaccg
    16141 gcccatgacg gacgcccggc gcgggcatgc cgatcccggc ggaaggcgtt ccccgcctcc
    16201 cgcacgggga gaccctacta tgaacggagg gctgaaaaca agcctagacc ttgatgaagg
    16261 tccggttttt gtacatgtaa tacaacacgc cccaaatcag ggcgtaattg cacaggtaaa
    16321 agacaaacct gtccgcatcc ccgaagcact cgctaaaccc ggcaaacaat acccgggaga
    16381 aattgcccgt aggggggcac agagataccc atatgtacac gaaaatggca ttgctcccta
    16441 ttacggagaa aaagaatgtc aaccagttca atttcagcac atccgtcagc agatagaaca
    16501 gggccagcag aaaatagcac catcccgccg cccacaatac catggaactg gtgaacaaac
    16561 gcttgatcat cggaaaatcc aggctccaga catatcccag cgccaggcag accgcgcccg
    16621 aacataacag ccacgccagc tttttcccgt gcccctgcac ccgcttcaga atctggccgc
    16681 ccagcagacc gagcatggtc atggcgccaa aaccgaactg cgccagaatc cacgcataat
    16741 tcgttccatc ctgccagttt cccatcaggt atttatccag cagcagggcc agattcctcc
    16801 ccggttcaag cattcctgcc gcacaggaac ccaccgcggg gtcagaaaag gaaacaaact
    16861 tcatgaccag ccagtacaca gccagcagcc cccccgttgc cgctacctgc caccggatgg
    16921 acagatgaag aaggcaaatg gccgcaatca ggtagccgga ggcgatggcc tggagcgtat
    16981 tgcagtacag ggacatcctg gacggttcaa aactcagcag attgccctgc accaccatgc
    17041 ccagcaaaaa aagcaccgct actcgcctgg caaccttcag gtaaatcttc cacaagggtt
    17101 ccgagccgat gcgtttggaa aaggaaaacg gcatggccgt tcccacaatg aacaaaaaga
    17161 ggggcatcac cagatcccag gcggcgaatc cctcccaagc cacgtgcgtg ctgtgcttca
    17221 ccagccactc ggggctccgg tcgtaaaaaa gattgatgcc ggccacaacc agagccagac
    17281 ccccggtcag gaaaaacatg tcaaatcccc gcagggcgtc aatggccgca attctctgcg
    17341 gcctggtatc ggaaagtgaa ctcatggcaa cattccaact acggcaccac gtccgccaac
    17401 ctttccaaag actttctttt tctaaggctc ctcaaagaaa aacggctgtt tccggaaaaa
    17461 cctgtccttc acggcaactc cgtcatgatg cctttctcca tattccctgc cccgcatttc
    17521 atgctatgct gcccctgcaa catgaccatg gggaacgacc aatccggcga acgcgacagc
    17581 ttccggaata ttgaaactta cgctcgcttg aaaatggatg aacttggtct ggcagactgg
    17641 caattcggct gggatcgggc caaaagaagg ctgggagtgt gccgcctgct ggaaaaaagc
    17701 attacgattt ccatccactt cgtccgcgcc aacctggaga cccctcatga aatccgtgac
    17761 acgattctgc atgaaatcgc ccacgccctg gcgtggaccc gccacggaga acgcacccac
    17821 ggaccgcgat ggaaacaaat ttgcagggaa ataggggctg tcccgtgtgc cgccgccaaa
    17881 ccggacgcca tccgcgtcac cacgtacaaa tatctcctgc gtctgaaaac gacgggagaa
    17941 atcgtcggca aataccaccg ccgcccctct ttcgccaaat acctcaagcg catggccctg
    18001 aaaggccgcc ccgatacgct gggccagctg gagctggcgc cttatgaaga ataatgtcca
    18061 caaaagctga aaaaacgagc cgtttccggc tattctccag catcaacgct catcccattc
    18121 cgggcatttc cattttttac gtctgctcat tatccggaac agcagaggaa acactcaagg
    18181 tgcgctggcg tacgtaatcg tacaccttcg ggtcgctgtc tccggctgcc cagaccaccc
    18241 gcaggaagtc ggcgggatga atatttccgt ggtcccggaa gaaacggcgg tcattctcac
    18301 agcagtaaat gatgtcatcc cccatggaac cgacgagctt ggcccgcgcc ttggcaatca
    18361 ggcgcggaag gtaggggaga ccgcagagtt cgtcgccgaa ccccggcaaa tcatacccga
    18421 tcacaggttt cccctggtaa aactgcccgt gctggacagt caggaagaag tcccggcgcg
    18481 ccgcagccat cagcagaacc gtggtggggg aaggttctcc cgtacgggca taggtttcta
    18541 caaaatcaaa caattcccga gtgcggcagc cgatggaaga cagaaagata atttcctggt
    18601 ccgtaaagaa gccgtccaca ttcctgtggc cttcatggta gcgccccacc gcttcgcgga
    18661 aaaggtcgta aaacgtatcg ttccaatctt cttccatcat cggcccggcg atttatgttt
    18721 gcgcttgaag ttaagcatca tgatctgctt ggtcagattg gcttcaaagc gggcctgttc
    18781 ctcgcgggac atttccgaac gcgtcttgag cacctcctgc gcttcctgaa tggcccgttc
    18841 cacgctgcct tcatcaatct gggaggaatt aagcgcgagg tcggttacca tgagaacatg
    18901 gtcgtcattg acctgcacaa agccggtgcc caccaccatg ctttcctccg ggccgtccat
    18961 gggcttgtag cggagttccc ctggttccag ggaggtaatc agggccgtat ggtgttccag
    19021 cacgcccatc tccccgagac tgccgggcag ataaacgtat tctacagtag cggcaaccgc
    19081 cacttcatcc ggggtaatga ctttgaactc catgctcatg gttcgggacg gcgggaaaag
    19141 ggttatttgg cttccttgag gacgtcatca atgccgccgc gcatgaagaa ggcgtcctcc
    19201 ggaaccatgt ccagcttgcc ttccaggatt tccttgaagc cccggacggt ttccgccacg
    19261 ggaacatagg caccgggaat gctggaaaag acttccgcca catggaacgg ctgggtcagg
    19321 aagcgctgaa tcttgcgggc gcggctcacg atgagcttgt cttcttccga aagttcgtcc
    19381 atgcccagaa tggcgatcat atcctgaagg tccttgtaac gctgcagcac catctgcacg
    19441 ccacgcgcca cttcataatg ctcattcccc acaatttccg gggccagggc cttggaagtg
    19501 gaagacagcg gttccaccgc cgggaagagc ccctgggccg ccagagagcg ttccagcacc
    19561 accgtggagt ccaggtgggc gaaggttgtg gccggggccg ggtccgtcaa gtcgtccgca
    19621 ggaacataca cggcctgcat ggaggtgatg gagccgtgct tggtggaggt gatgcgttcc
    19681 tgaagatccg ccatttcctc cgccaggttc ggctggtaac ccacggcgga cggcgtgcgg
    19741 cccagcaggg cggacacttc cgaccccgcc tgggagaaac ggaaaatatt gtcaatgaac
    19801 agcagcacgt ccttatgctc ctcgtcacgg aagtattccg ccatcgtcag ggcggacagg
    19861 gctacgcgca gacgggcgcc ggggggttca ttcatctgtc cgtacaccag cgccaccttg
    19921 gactcttccg gtttctccag gttgatgact ccggattcta tcatctcatt gtacaggtcg
    19981 ttcccctcac gggtacgttc ccccaccccg gcaaaaacgg agaggccgct gcgcgccttg
    20041 gcaatgttgt tgatcaactc catgatgagc acggtcttgc ctacccccgc acccccaaag
    20101 gcaccgattt tgccgccttt caggaagggg cagataaggt caattacctt gatgcccgtt
    20161 tccagcactt ccgtgctggt ggattgttcg tccaacggcg gagcgccgcg gtggatgctc
    20221 ttcataccgg cgcaggagag tccgccgcgc tcgtccacag cttcccccag cacattgaaa
    20281 atacgtccca gcacgcactg tcccacagga acttcaatag ggcgccccgt gtcgcgcacc
    20341 ggcatccccc gccgcagacc gtccgtagaa ctcatggcca cggcgcggac ccagccgtct
    20401 ccgatgtgct gctggacttc cagaaccagg gtcttgcgct tgccgtcaac ttcatagtcc
    20461 acttccagcg cattgagcag ggcggggagc gtttctgcct gggagaagtc cgcatcgacc
    20521 acagcgccga tgatctgaac gagaatgcct gtgttattca tcatgggaaa aacttggtgt
    20581 tgaaatggag aggaaagaat gttctgtttg aaaagcgggt aaagaaagcg gaaccatgtt
    20641 attccatagc gcgcatagcc gtggtaattt ccagaagttc gttggtgatt tgcgtctggc
    20701 gcgccttgtt gtaatccagg gtgaggccgc cgataatgtt tttggcgttt tccgtggccc
    20761 ccttcatggc gaccatgcgg gcggagtgtt cggatgcccg ggcctccagc acaatctgaa
    20821 ccagtccgtg gaaaacgtaa agaggcagga tggtatccag cagagcagcg gggctgggtt
    20881 ctagcaggaa ttgcttatgg gcatctgctc cgtccaggga agagcctccg gctttcgcca
    20941 tgttcatcag gggttccggc tcaatgggaa gaagctggcg aaagacaggc ttctggacca
    21001 ttgtgttcac aaaaccggag aaagccacga aaacgcgccc atattcctcc gccttgaact
    21061 tttgcacgat gaaatcaaat accggcttta gttccagcag ggacagggga tccgtcaggc
    21121 tccaggtggc ctccaaccgt tcattcaatt tggcgagcgc agcattcagc ttgcggccga
    21181 tggtgatgta atgggcgttt tccggggcct gttcccgaac cattttgatc aggttggcgt
    21241 tcagcccgcc gcaaaggccg cgatccgtat tcaccagcag caccagatcc gcgccgccgt
    21301 gggtttgcat cagagggctg ttgctggtgt caatttcatc ctggagatgg tagagcactt
    21361 ccgccagggc gcggatatag gggcgtccct tgacggcctg gtcctgggcg cgacgcattt
    21421 tggcggaagc caccatctgc atcgccctgg tgatctggga cgtgtttttg acggacttga
    21481 tccggcgtct gatgtctctc agattgccca tgtgtcagta ctcccgtaat cgttagttcc
    21541 agctgagctt gaaggattcg atagcggctt taagccctgc gtctatttcc ggcgtcaggt
    21601 ctttttcctt cagaatgctt tccagcagat ccggatgctg atcctgcatc accgcttccc
    21661 acgcattctg gcattccttg atcctgtcca ccggaacgtc gtcaaagtac cctttttgca
    21721 tggcatacaa gtcagcggcc tccagaccca gggatttggg ctcatactgg ttctgcttaa
    21781 agagctccac gatgcgctgg ccgcgggcca gcttggcctt cgtgctgggg tccagatcag
    21841 acccgaactg ggcgaacgcc tgcaattccg taaattgggc cagatccagc ttaatggagc
    21901 cggccagctt cttgataatt ttcgtctggg cggaggaacc cacacgggag acggaaatac
    21961 ccacgttgat ggctgggcgc accccctggt agaacaggtc cgtttccagg aatatctggc
    22021 cgtccgtgat ggaaatgacg ttcgtgggaa tgtaggcgga aacgtcgcct gcctgcgttt
    22081 caataatggg aagagccgtc agggaaccgc cgccgtgttc cttgttgatg cgcgcggcgc
    22141 gttccagcag gcggctgtgc aggtaaaata cgtcccccgg gtacgcctcg cggccggagg
    22201 ggcggcgcag aatcagggaa atctggcggt aggccacggc atgcttggaa aggtcgtcat
    22261 aaacgataag ggcgtcttcc cccttgtcca taaagtattc ccccatcgca cagccggcgt
    22321 agggcgccag gtacagcatg gcggcaggat cggaggcgga agccgccacg acgatggaat
    22381 atttcatggc tcccgtttcc tccagtttcg ccaccaggcg gctgatgttg gcgcgcttct
    22441 ggccgatagc cacgtaaata ttgtagagag gctggtgatc aggaagcttg ccttcctccg
    22501 ccagacggtt ctgttccgcc tgggcaatca tggtatccat ggcaatggcg gtcttcccgg
    22561 tggcacggtc cccgatgatg agttcgcgct ggccgcggcc gatgggaatc atggcgtcaa
    22621 tgggaaggat gcccgtctgg acgggcacgg aaacggattg gcgggagatg atgccggagg
    22681 cgattttttc caccgggtaa tacgcttccg cctcaatcgg ccctttgccg tcaataggtt
    22741 ctcccagcgt gttcaccacc cggcccagca gggagcggcc cacgggcacg gaaagaaggc
    22801 gtccggaaca cttgaccaga tccccttcct tcaacttgga atcatccccc agaatcacgg
    22861 cgccaacctc atgttcttcc aggttcatcg ccagccccat caccccgccg gggaactcta
    22921 tcatttcatt gagcatgacg ccgctcagcc cttcgatctt ggcgacgccg tcccccacgg
    22981 aacgaatgac gcccacgttt tcctgactga cggaagccgt ggcttttttt atttctgctt
    23041 cgagttcttg aagtatgttg ctcatgggaa aaattcgttg gttaattgga aagggtgcgg
    23101 gccatcgcag ccagacggtc gattctggaa cgcacggaac cgtccttcac gtcgtcccct
    23161 acctggatgc ggacacccgc cagcagagac ggttccacct cccagcggta gtagaggacg
    23221 ccgccatatc tggcctccag tttggcctga atcagggaac gctcctcttc cgtgaggggc
    23281 acggcgcttt gaatagtggc tgtacggctt ttcaccgcat attccaccat gccggaaaaa
    23341 gccttcagca gagcggcata attgcggggc ttgcgttccg caattttccg ggcgatcaga
    23401 cgcacgcggt cctccgccac ggcattcnnn nnnnnnntct ccgctggtct tcttccgtca
    23461 gcatttttcc ggtaacctgg gccgccgcca gggccaccag ctggccaaat tgttctttta
    23521 acgcttcacg ctctttctgc tgttccagtt ccgcaagctg gcgggccttt tttatgattt
    23581 cctcagcctt ccgggaggct tccgcttcct gttcttccaa caggcgggcg gcggcagcct
    23641 tggcggcgtc tattttttcc tgtccctttt cccctgcttc caccagcatt tcatgcgtct
    23701 gttcctttac ggaagccagc tgccgctcac tttcctcacg catttcctct ccttcttcaa
    23761 tacgctggcg ccttttttcc agaacattct ggatgggttt gaacgccaga taacgcagga
    23821 tcaccaccat caggatgaaa gcgatcaggt tggcaacaaa gggttcccaa ctcgttaccc
    23881 caaagggagc aaaaggattc caggcttggt cagctatgag attcagcata agatgagcgg
    23941 tttgaacatg ccggaaattt tccaaaaccg gcagggaacg ggcgcaccgc cccggagcgg
    24001 agaagctccc tccgggggcg aaacttccgt tttatttcac aaagatggag atcagggcca
    24061 cgccttcaat cagggccgcc agcgtgatgg caatcaccat aatgggggag gaagctccgg
    24121 ggttgcgtcc ggtggcttcc gcggctttcg caccaatgag gccaatgccg aggccggcgc
    24181 cgatggtgac gaggccgaaa cctacgttgc cggacagttc ggccagggag gtcattgcga
    24241 taggatcaat catgttattg gtatggttta tgttttttgt tgaacgttcc cccacaggtt
    24301 gcccatggtc cggaacggaa agctggcggt acggtttatg aggtttttcc aggcggcagg
    24361 cggtccggca cgggaagttc cttctcctca tttttggaag catggccgtg ggcgttatcg
    24421 tcccccacct gaagtttcag gaaaatcgcc gtcagaagca ggaaaaccag ggcctggatg
    24481 aaccccacca gcagttcgat cgccagaaac ggcagtacgc acagggagct gagcacgggc
    24541 ccgaacatat gggccatggt gtcaataatc gtttccccgg cgtaaatatt gccgtaaaga
    24601 cgagcggcca gcgcaatggg gcgaatcgtg atgctcagga catccaccag tcccacaaaa
    24661 atgaagatga acaccagcac gaatcccatg aaccccggca gtctcccctt ggggccgaac
    24721 aggtgaataa agaagccttt cagcccctgc tcccggatag accagtaaag ccacatcagc
    24781 gcataaaaaa tacccaggaa catcgtcacg ttcatgtccg cattcgcgcc gcggaacagc
    24841 ggcaccccat gataggtgat ggttcccacc ccgggaagaa gccccatgta attgctgacc
    24901 agaataatgg tgaaaatagt tccgaagtac cagaagtact tgcgcgtcag gcgcggaccc
    24961 agaagggact ggacaaagtt gtacagagtt tcaaaaaagt attccaccgc attctgtaaa
    25021 cgggaaggca ggatgcgcat agagcgggtg gcggaccata cgaccacccc tatcaaaaga
    25081 accgccagaa aaagcatcag catcgagttg gacaccggga gctcaatgcc tccgggcaga
    25141 ggtatggaaa acaaatgggg agcggcctgt tccaatccgt gctccgctcc ttcctccgcc
    25201 gcacaggcgc tgccgcccat aacgccggac acggtcaatg ccgccaacca caggaactgg
    25261 aaaaatctgc tcattgtctg tcttttacca ttactcgtgc caactcctgt aaatactgga
    25321 aaaaggaatc cattcccttt atttgacggt tttgattgtg gactctcctc tttattttgg
    25381 caagcccaaa actcggcacg cacacaaatt atatacattt gtacataatc ttgaaagaaa
    25441 cctggatgcc cgttacaaca tcaacaaaat cttttctcca tcaacaaaaa agcttccggg
    25501 aatccatccc ggaagcctgc aggcagaact gaagggccgt tacttggcgg cggaaataat
    25561 atggatgtgg tacacgtctc tgccgtccgc ggccttgcgc cccgtggcat gaattcccgt
    25621 aacatcggaa gtgattacat cgagggtttc cacagcattc cgaagctccc tgctgctttc
    25681 cgccgcgctc ttcagggcgt cacggaccgg aaccaggtta agctgaaatt cgagaggggc
    25741 tccctgtacc ggagatttca cggattccct gaggacttcc ataccgaagg ccttcggcat
    25801 actgaccgcc cagcgggtat cggaaatgct taccaccggg ttcagatcgg cgcccaaagg
    25861 acactggtaa tcgtaggtag tgatatcccc gtccacagaa gactggggag cgggcaactc
    25921 cgccatttta ccctggctgg ccagagtgac gacggttttg gcggcattag ccactttctg
    25981 ccaggcttct cccagggcgg cgcggttttt cacttcatat gcagcggaga aacgcggcgc
    26041 cggcacattc ttgagcatgg gactgggagc gccctgcatg tccataatga acacgcctga
    26101 acccgtaagg ccggaaagag cagttttata agccttccag agctcctcaa tggtaggcct
    26161 ggccatctgg atcatagggg cggccatgcg cacctgatcg ctcacatcat gcttcggatt
    26221 ggaaatatag gcattgccgt agtcccatac aatctgggca atattaccgc acagggaaca
    26281 acccatatcc atcatctgcg gattgaccgt gcaggaggaa tacagcaccg tggacgcgcc
    26341 aggacgcacg gcactcatgg cggaaggagc gtccaacttg tagaattcca gcggatatgt
    26401 gcatgcttcc acatgcaccc cctgatcctg ccacgagaag taggaaacgg ccgtagcatt
    26461 ccgggccagc ttttcataca tgccaagcat gctggccttg atggaatcca gcgccgtcat
    26521 ggaaggagcc agatcggcaa ttttccaatc ggcgcctatc gtctgcatca catccttcaa
    26581 cccggtgagc atgcccttgt aataggccaa atccatgtta ataacccctt tgacggaagc
    26641 tttgtctgcg aacagcagcc cataagccgg atactggaac cgtgcatccg ccatggagaa
    26701 atcaggacgg gacagcatgg attcctgggg tgaagaggcg atacggacct gtttttcagg
    26761 attggtggtt ataaagccta ccagggtatc atccacaaaa gagacggcca catacagcct
    26821 ggattcatcc agacgaacga gcgccgcctt aagacggttc atattttccc gggaaatctc
    26881 catccggtct ttcgcctcat ccagcatttc ttccagcttc gcacgcactt ttttgcagtc
    26941 cacttccgct accttgcagg ccaggccatt gtaggtcttt tcatacaggg aaaccattcc
    27001 atgcagctcc atatcctcct ggagattggc gtccttcagg aaagctttgg cctgggcctg
    27061 ggcttccggc gtcagtttgg cggcaatcat cacaggcgcc gtcgcagcct gggaagggcg
    27121 taaatccatc agctcagccc actgcacaat catctcggcc ccctggccgc ccatgacctc
    27181 cgcccccgca ccatcggcat cctcatttcc atcctttacc ccctggaaaa gcttgtaaac
    27241 ggacaaaatt tccttcatct ggttcaagcc catcttggta aaagaggact gcgcgctatc
    27301 cagccacgga gcccattcgg gacccaagcc gatcatcgcg tcaatgacag ggctgctgaa
    27361 agaacatttc cgctgcacgg gttcggaagc gtcttctccc ggaatggcta tggattcatc
    27421 ggcctcctcc ccggaggagc aggaactcca ggttttcacc atgttcaaat tctgaacgct
    27481 cttcatgatg ccgggaatat cgtaaacacc cgccatcatc cccatgtcct tgggaaagag
    27541 agcggctatt ccgagccgtt cggcacgctt cgccaccagg tcggaagcca cggaagccaa
    27601 aggatcaaga gaggcggcgg gagtcaagcc ggctgcatcc tgcccggcct tggcggaaga
    27661 tgattgatga tcgtcagagc aggcggcaag agcgcaggtc agcacaaaac agggtatgga
    27721 gtagaaataa tgctttttca ttatctttaa acatggggtc atgatgccat ccggcaagcg
    27781 cgctgcttcc ttgcggcaac agcccctatc ggcgcatctt tcccattata caggcgtttc
    27841 cccgcacgac gcaagcacaa tgcaaaacat taacgcactc aggcgtgcat gacgttgctg
    27901 cgtgaaaaac cttttaagcg ccgccagcta tccccgtatc cgccagattt tcctttttcc
    27961 cccgccaata ttgtccggga aaaagccgtt ccggaaaccg caaaaactct ccggctttct
    28021 ctaagaaccg gattaaagag gttccgccat gggaatatca tcctccggca ggggagactg
    28081 cggcgcagga gtgtttacgg aggtggaacg gttaagatgg cggggaatct gctggggcag
    28141 tccggggatg taatcggaat caccccggcg cggcgtgagg ggggccgcag aaggctcctc
    28201 cgacaacgga ggcgccgacg gcccggcagg ccttgaggag ccgcccaccg gagttgcctt
    28261 gatttcagca tcggaagagt catccagatc ggcagggatt tcatcagcct tgatcggctc
    28321 catcccttcc ggatcataca agccgggcgg caggttgtcc ggcacgacgg caggcgctcc
    28381 gctccgttcc cgaagagcgg ccgcagccgt ttccggggtc tcctccaccg gttccgccac
    28441 cgggatgtca tctttgggcg ggtcaataat agccttgatg tcttttttct ccgtttcgaa
    28501 aaattccttt acgatgggag cggcggcagc ccccccgccc agccgttcgt gggcgcggcc
    28561 ttcatacagc accacatagg caaagcgcgg attgtcgcag ggcatgaaac cggcgaacca
    28621 cgccagacgc ttgtcttccc gttccggccc ccattgggcc gttccggatt tgccggcgat
    28681 actggcataa gacaggcgcg cacgcccccc cgtaccggct tcaaccactt ccctcatccc
    28741 cttgcgcacg ctggacagag cacgctcata ggcaggcaga ggacgttgaa cttcctgggg
    28801 cttgggcgcg taaacgacat tggcgttgcc gtcctgaatc tgcttgatga gctggagcct
    28861 gggcagatac ccgtttgcca cgccggacat catgtgcgcc acctggaggg gcgtaaccag
    28921 cagcgtcccc tggccgatgg acaaattggc agcatccccg gacatgaaat cgcgcttgta
    28981 attgcggatc atataatcac tggtcggcac caggccggga tcgtccggaa tgggcaggcc
    29041 ggtccgttcc cccaggccga aggcgcgggc cgtatccatc agggcttccg cacccagctt
    29101 caggcccatc tggtacatga acggattgtt ggacatcgcc agggcgcgga cacagttgat
    29161 atcccccagg ggagtcttgc tccagttttt gaacacgtgg ttgccgatcg tcacggaagc
    29221 cgggcagtaa acgagcgtat tttccgtgat tttgttgttc ctcaaggcac tggctaccgt
    29281 caggacttta aatgtggaag cggggggata acggccctgg aaagcacggg cgcccagagg
    29341 accggcagga tcattgcgca aagcatcgta atctttctgg gatatgtttg gaatgaacat
    29401 gttcgggtca taggacggcg tggacgccat caccagaact tcacccgtca cgcagtccag
    29461 caccaccatg gcgccgcgcc tcttgccgcg ggaaaggatg cgttccgcat ccttctgcca
    29521 tttcaggttg agggtggtga cgacagcccc gccgggcttg gggcgtattt gcagttcatc
    29581 cagaatttta ttgccgtctt cgtcaaacat caagcgccat acgccgggcg ttccggtcag
    29641 atttttgttg aattcctttt ccagccccgc acggccttcc acctgctccc acagcggatc
    29701 catgtggttg atggggccgg ttggcagttt tcccttggac cccacatagc cgatgatatg
    29761 gccggcgatt tccttctccg gataggaacg gatgtaaatg ggcagcagct ggaggccgcg
    29821 cacacttttc accttatcct tcagcttttc cgcttcctcc gcacgaatga cgttcgtgag
    29881 cggaagaggc agccagcggc ggtgctcata atgtttccag agctgttcgt cggaaaaaga
    29941 ccatgccttt ccggcaattt ttttggcctg ttccaaacag ttgcgcccga attccaccac
    30001 gcgggaacgg tcggggtttt caaaaatttc aaaccgcaaa gccagctggt aggctacgga
    30061 agtcacggcc agcggttccc cgttgcggtc caggatgggg ccgcgcggag cgggaatcag
    30121 cagagccatg gtcctggcgg aaggccgcgt gctggtgtgc gcttcccgcg cagcgtccaa
    30181 ccgacctccg ccaagggaag gaatcaggga cagggaatcc aagccattct gctccttttc
    30241 cgtttccctg ggcatctccg gagcggcgac cacaccttcc tgttcgccgg aagccgtgtc
    30301 ggaatccttc tttttgtccc ccaggtcaac cgtttccacg gcgtcatcct ttccgctctg
    30361 ttccaccgca gcccggccag cggccgctcc cccccgggaa gacgggcggc gcgtggaagc
    30421 ctggccccag gccgtatccg ccgtccacgc ggccaaaccg gccaacaata ccagggaacg
    30481 tatcagaacc tttattttca tcagcttgca ggattgtggc aaatataccg caaggaatca
    30541 acgattattc acgctcacgc ttattcctga acgatgacgg ttgttcccag gccgcatttg
    30601 tcaaacagaa tgcggcatgc ctccaccgga acccggatgc acccgtggga attggcgtcc
    30661 cggaacacgg tgcccacatg cagccctatc ccgccgtgaa tgcgcatcca aagaggcatt
    30721 ttggcgccga tgaatttacc gccgggaggc acgcgggaag aagaggtagc atccgaattc
    30781 acgcacaaac cgcttgcatc aaacacgctg ccgtaagacc gggaacggta atccgcatct
    30841 ttttggatga tggaaaactt gccgcgcggc gtttcatggg cggatttgcc cgtgcaaacg
    30901 ggaaagttca tagccggctt gccatcaacc aacaacacgc cgcgctgatc cttcagatga
    30961 atgacgattt tgctgttccg cgtccccgcc tggcggatac gctcgtcatg ataccagata
    31021 tcgcgcgtga agcggtactg ggggtccgcg acaaaagctt cataggaaga agcatatctg
    31081 ccgtccttca tcaacggctt tccatgaggc gtcctgggcg tggaggaaca ggcagccagc
    31141 agcagggaaa cacacaggaa aagaagaaac gggagagcgg cgtgcggcat gcggcctgct
    31201 ttaccctctt cccccgtgga aatcaagcac gattcccctc cgttccggca tgtttggagt
    31261 aatcctgata aagacgcccg tttatgcccg tatctgtttc cgttctttct ctctgccgtc
    31321 atgaatgccg cttcttccgt tcccacccgt tctccccgcc tgcactccct ggacgccctg
    31381 cgcgggctgg acatgctcat tatcctgggg ctggacgccc tgattctgct tctggcctcc
    31441 agaaatccgg aaaacggctt cctgcaagaa gccgcgcgcc agatgaccca tgcccgttgg
    31501 gaaggattac acctgtacga tctggtattt cccgtctttg tattcatatc gggcgcctcc
    31561 atgtccttct ccctggcgaa atacaccggc acctccatcg cccccggact cttccatctc
    31621 tggaaacggg ctttcggcct ggcttttctg ggccttctgg tcaacggaac gcttacctgg
    31681 acggaaagca tgcgttacgc ctccgttctg ggactgatcg gtttttcctg cgccattgca
    31741 ggaacctgca tcctgctgtg ccgccggacg ggggcggttg cctcagcagc gggattcatc
    31801 ctggtcctca ttaccctgct tcagttcacc tgcggcaatt ttacgccgga cggctccgtc
    31861 aactcatggg tggacaggca ctggctcccc ggccgtctgc atgggggagt ttttgacccg
    31921 gaaggcccgc tctgcgtcgt ttccgcctcc gccctgtgca tgggaggatg gatagcggga
    31981 aagtttttga aagaaagccg gctccccccc gtgcgcctct gcacggcaat ggcaggagcg
    32041 ggccttctcc ttttctgcac cgccaggatg ctggacagca cctaccccat cattaaaagc
    32101 atgtggacag ggaccttcgt cctggccgcc gccggcattt ccctgacgtt tctttccctg
    32161 tttcatctgg ccgttgatgt gtggaacgaa gggaaatggg cccaatgggc atttcccctc
    32221 cgcgttatcg gcataaacgc cctggccgcc tacctcatcc actccctgct caatgtgcat
    32281 gaacttaacc gtcgcatctt cagcggcatg gcagacctct tccccccgtt ccagcccgtc
    32341 ttccttgccg tgtcccttct gctgctgcaa tggctggcct tgctcttcct tgacaggcgg
    32401 cgactgcctt tcatcaaaaa agcggtcaaa tgaccaggcg gcggataccc cggccatggt
    32461 tgccggtgct ccccgaaaaa acggatactc ccctgtgaaa cggccagcca acattatctc
    32521 tttcccccaa agccctaaaa ggagaaataa agcggaaaca tcattttttg ctcgtttcag
    32581 ggaaaagctc tgccaccctt tccggaaaga ggccaagcac gttccttcca tgattgtctt
    32641 ccggttcccc tgccccattt cctaacccca tgatcattcc atgaaagcat tcatgttccc
    32701 agcccttgtt ctggcggccc tgaacatatg gactggcgcc gcccagcccc ggacaaccgc
    32761 cattttacct gtaccgggcg aaaaggcgtg ccggcttccg cttcctccct accaggcctt
    32821 ttgctggttt tcagcggacc gcggccgctg gcccggagac ggcaaccggg ttctgccctg
    32881 gttcggaagg acggcccccg gcaacctgct ggacagcaag ccgaatccca gcaccgccaa
    32941 accgggggac catgccatgt tcgccctgtt ccacctcaaa gacggcaatt tcatggccgt
    33001 gctccccgtc gccgcccctg attccctcgc gtggctgaaa cttgaaaggg acggaacatt
    33061 ccttgtggaa gccggttcgc tgggaacttc ccccgccaaa ccgcaggccg tcctggccgt
    33121 caccgccacg gacaaagata tttaccgcgc ctgttccgcc gtatgggaca aggccctttc
    33181 cctccctttc atcaagggca gaaccttgcc ccgggaaaag aaaatttatc cggaaccgtt
    33241 caagtacctc ggctggtgca gctgggaaca gtataaaaag aatatttcct ccaagctgct
    33301 tgaagaaacg gccagaaaac tggaggcatc ccccgtcccc gtccggtgga tgctggtgga
    33361 cgacggattc cagacccagg aaaggcttca gctggtcagt ttccagcccc ggcaggatca
    33421 attcccccgg ggctggcagc ccctgatgaa acacaaaagc cccaaattga aatggatggg
    33481 tctgtggcac tgctattacg gcctctggaa cggcatccac cccaggcacc gtctggatga
    33541 cgagactgcc cgcggtctgg tccgtaccgc caaagggaaa atcctccccg gtgacggctc
    33601 cggaggagcc ggagcgtttt ataccccctt cctgcaatcc gtcaaagacg ccggattcga
    33661 cttcgtgaaa attgatgtgc aggcggaata cctgaagcat gcggacgggc tggacaaccc
    33721 ggtccgccac aataccaagt gctcggaagc cctggaacag gcctgcctga aaacagggct
    33781 cagcctggtc aactgcatgg cgcagggtac cgtcaacatt cagaacacgc gttacagcgc
    33841 ggtcacccgg tgcagcatcg actacaagct gggagatgaa gccatggcca aatcccacat
    33901 cctccagtcc tacgccaata ccctctggct gggacagacc gtgtggccgg accatgacat
    33961 gttccattcc acggatcccg cctgcgcccg cctcatggcc gtatccaaag ccgtctcagg
    34021 cggccctgtc tatctttcag acccggcaga caaacttaat ccggaaaaca tcatgcctct
    34081 cgtctggtcg gacggtcttc tgctgcgccc gctggctccc gccgtgcccc tgcctgattc
    34141 cgtctttccg gatgctctga acgagaaccg cctgtaccgg gtaatcgccc ctttacccgg
    34201 tcaaagcgcc gccgtggtgg tgtacaacct caaacatcct tccccggcgg aaccggtacg
    34261 ggggaaaatt tccctggagg attataaaaa cgcggcggct ctgctgaatg gcaatgcggc
    34321 ggaagcctac gcctctctcc cggcggaggg catcgccgcc tattccgcag agggaggccg
    34381 cgcgctgact ccggcacagc cggatctgga tgtagagctg acgggattca aggaccgcct
    34441 gttcatcatg gcccctatcg tccaaggttg ggccgtcatc ggaaggaggg acaaatttct
    34501 ctctccctgc gccctggttt ccgctccggg gtacagggag aacggcctcc gcttccgcgt
    34561 gaaggaatcc gggcctgtcg tcatctggcg gggaaaaggc ccggtcaaag cgggaaacac
    34621 ccccgtcagg aatctgggga acgggtttta tgaattgcaa tttcccgtct ccgaccaccc
    34681 cctggacatc actgtaacag cggaataact ccggacattt cctctcattt ttcctacttt
    34741 gtctcccatg aacccgcttc atcccctcct tgctttctct ctgttgcttc cgctctgccg
    34801 gcattccatg gcttccgacg cagctccgac tgccattccc gcgccgaagc ccgaggcccc
    34861 aaggcaggca attgaacaat tcctggggct taccgccatc tggatgctgg aaatggaatg
    34921 gacccacgcc tatgccaata aaacgcccaa ggcggaggat ttccggaatg ccatgctggc
    34981 ggcgggcctt cagcaatgcc ccgcggactt tcaggaagca tggctgcggc aaacggccag
    35041 gccgggccgc aattacgcgg ctcccgtcct gcgcaagtac ggcgtcaggc tgaaagacct
    35101 cagggaaagg ctccagggca aactgttcaa aatcaaccct cgggtgcatc cgcccattcc
    35161 cctttacgac gaagatgaac aaattccacg catggatccg cgcacatgcg gagaccccaa
    35221 ggctatcctg gaagccctgg ccgccctgcg cgcccagatc atgggcctca ccccgggcca
    35281 gctggcccgg gccagacagt ccattgaacg ggttatgctg gaattcacgc tggcgtacat
    35341 ggaaaccgcc atctgcatgg atactgcggg catccgggaa agccaggtca gggaactgtt
    35401 cgcctccatc agaacagaag gatgcccgga agacttccgg cgcgcctggc agcacgacct
    35461 gcctttcttt ctcaaaggcc gcttcacggg actggaactg actctctctg ccgtctgtaa
    35521 aaaatacggc gccgacgagc aggaactctt tctcaaggtg cgacggaaaa tgaaggaatg
    35581 ggatatccag ccgcctacgc cgcagacgca ggacgccttc cgccgggaca tgcgggaaat
    35641 ccgggaaaac atgctgggag gcagatagga aacagccttt ttgcatgcaa cgccttccgg
    35701 gaaccttccc ggatatcnnn nnnnnnnata tcaggccaag gtcaccccag cagcccggtg
    35761 tccattcctt cgttatattc ccaaattccc tgctccacca taaacgcgct ccagctattg
    35821 agcagttgtt caaaataggc ggcattcaca ttgaaatggt aaaatgtgtg gtccgccacg
    35881 tccagaagtt caaatcggga acggttcttc ttttttctcc acagctccgc cagcctctcg
    35941 ctgttgcgcc acggcagaag cctgtccctt cctccatgac tcgcaaacag gggaggcagc
    36001 cccctctgca cgcgccgcag ggggttgacg gcatccctct ggtccggagg catcatgccg
    36061 cccagcttaa aagcggattg cgccgtcagg tcgcacacgc ctcggaacag ggccacggcg
    36121 gcggggccgg gaggaagggg agcctttttg cggaacaggg accagcgggc gggatgatcc
    36181 ggaagggcca catgcagagc catcagccct ccggcatcac ttcccgccac agtaatgcgg
    36241 gccgggtcca gccccagcag agcggcgttc ccatgcagcc aggcccaggc ttccctgccc
    36301 tcttccagaa tgtccatcgg ctccacttca taatcgccgc gtgtgcggaa catgggtata
    36361 atgcctgcaa taccctgctg ggcaagatgg agcgcccacg gcacaaaatc ccccaggctc
    36421 ttgcccaccc acatgccgcc gtggaaaaag agcacggaag gagcggcggc ttccgcccgg
    36481 tggccggggg gaaaaaagac atacgccatc agctcctctc cgtctgaaaa ccgcctccac
    36541 acaaagccgg gctgttcccg caacagtttc ctgtaccggg cttccgccaa atgatcggct
    36601 tccgtgaaca attccataag aacggcttca tgatgcaatt gtgttgcact ccgcgcaagt
    36661 ttgatgtcta aataatcgtg taatgacttt ccgttccatt ttctccctgg cctgctgcgc
    36721 cgcagccctt ctttctcccg cttccgcagc cccccgcccc ggagacgcca aactgagaga
    36781 cgacctggac gtggcttata atacatggcg tctgcatctg ctgcgcggca attatgacgg
    36841 ctggagatcc acaacctccg cctaccgcca ggtgaaagtg cgcaatctgg ccgtctcgga
    36901 aaaacgcccc tttcccgctt ccctgttccg gcagcccatg gcacctcctg cgctcgctcc
    36961 gctgatgtac gtaggttccg tcgtaaacgg ccccacggcc gcagccacct actacggcaa
    37021 ggtggatctg gggctgggcc aggaacccac ggattcgaac gccctggtgc tgctgttcac
    37081 tcatgaaaac ggcaaatgga aatatgacca ggcgcgcttt ttcaacctca cccgcctgcc
    37141 cgccgtcaaa gaacgcctca aacgcggaga cgcctccgtg ctgatggagc aggacgggtt
    37201 ccagcccctt gggaaaattc ccgccgtgcc gcctgtctgc ccgccgccca aatacatcgc
    37261 aaaaattctg gtggactgtc ccggccgcac cgtccaggcg aacgtaaaca atatttccct
    37321 gcatgaattt gacaacaccc gcctggcgga agtcatttcc ggaggactga gggacgggac
    37381 caactcactc acactgaact tttccgacag cccgaacggc aaaaaaggcg ccgtactcgt
    37441 ggaagtgtac atcatgccgg aaatcccggg ccacctgccc gccagggctt tttcctactt
    37501 cgtccccccc caggcgcaac ccaaaagcgg ccctgtgctg attaacgtca ctccggaatt
    37561 gctgaagacc atggaaccga agaattcttc cccgaaggca gccgccggca aataaaatcg
    37621 tcattcttca acgtttccgt tgcgggcccg gtcataccgc catatgacaa ctcaacctca
    37681 agtcagcagc tccgcggtat ccggcaactc atggtggatg ggaatccttg gcgtcatcga
    37741 gctatttctt ggcttcatcg ccctggcctc tccctggatc gtcggagcca gcttcatttg
    37801 ggtaatcggc atcatgctga tggttctggc cgtcgtccgg ctgatccagg tcttcaccgt
    37861 cccttcctcc cgtgggtgga acctggtgac ggccatcctg tacggcatcg ccggctggtt
    37921 tctgttccgg gaccccaata tttccctggc tatcaccacg ctcatcatcg ggtggggcct
    37981 ggttattgcc gccgtatttc agggagccat ctggcttcaa acccgttccc tgcctgccag
    38041 cgggtggcgg ctgttcaacg tgatcatcac gctgatcctg ggcctcatgg tcatcttcgg
    38101 ctggcctgaa tccacggcct ggttcgtagg tactctgatc gcagtggaac tgatcttctc
    38161 cgggtggacg ctgctcctgt atgcattcag cggcaattcc gcccgccgtt aatcccagtg
    38221 acaacaatga ccctcaccca ggaagaaacc gaacggtacg ccaggcatct ttcccttccg
    38281 gaactgggag aacagggcca atacaaactc aaacgggcgc atgtcgccct gacgggcctc
    38341 ggggggcttg gctccccggc ggccctttat cttgctgccg caggcgtggg gcgcctgacc
    38401 ctgattgacc cggacgaggt gtgtctttcc aacctccagc ggcaaatcct gcatgccacg
    38461 gatgcggcgg gaacggctaa aaccgccagc gccgccagac gcctgtacgc cctgaacccc
    38521 tccgtacgca tcaacacggt ccacaggcgc cttacgccgg aaaatgccgt ttccctgctg
    38581 gaagggtgcg acctggttct ggacgcttca gacaattacg cggcacgctt cgccatggcg
    38641 gatgccgcct gcaccctgcg cattccgctg gtgtacggcg ccgtgaaggg ctttatcggg
    38701 caggtggccg ttttcgcccc ccatcaggga accgcctgtt accgctgcct cttcccggca
    38761 gatacgccca tgcaggaaaa agacaccgcc tccgccgcag gcattctggg tgcgcatgcg
    38821 ggcatcatcg gctgcatcca ggccatggaa gccctgaaat atcttgcggg cattccctct
    38881 ccgctggtgg gcgccatgct ctctgcggac acgcggcgca tgcgcttcac caccatccct
    38941 ctggcgccta atcccgcgtg cagatgccgg actaacgagg ggtgcggagc cgcaatgaaa
    39001 aattgacggc gacggcggca tctggcatca tttccggcag gtatgaatca tttaacacgc
    39061 gccaaatccg tttttgaaat ggagattgaa gagctgcgcg gcgtcctgtc ccggctggac
    39121 gacaatttca acaaggctgt ggacctcatg tcccaggctc tggaccgcgg caacaagatc
    39181 gtcattgtag gcgtaggcaa atccggcaac atcggggcca agatcgtcgc taccctgaac
    39241 tctacgggaa cgcccaccgt tttgctggat tccctgaacg ccctgcacgg ggatctgggc
    39301 atcgtccagg acggggacgt ctgcattgcc atgtctttct ccggagaaac ttcggagctg
    39361 ctgaccctgc tccccttcat caagcgtttt gaactgccca tcatttccat gacgggcaac
    39421 acgggctcat ccctggccaa atactcggat atcgttctgg acacaggcgt ttcccgggaa
    39481 gcctgcccgc tgaacctggc ccctacttcc agcaccacgg ccatgctcgt catgggagac
    39541 gccctcgcca tggccctggt ggaagcgcgc cacttcaccg cgcgggactt cgccaaacgc
    39601 caccccggcg gttcgctggg gcgtgccctg ctcacccggg tgagcgatat catgcgccgt
    39661 ggagaagaaa tggccatgct tccggaaacc gcctccgtga acgactgcct gaaggcgatg
    39721 accacagccc acgccggtgc ctgcgtcctg ctgacggagg accgcaagct ggccggcatc
    39781 ttcacccacg gagactttgt ccgggcatac ggggcgaacc ctctcatcgg agaacagccc
    39841 gtcagcgggt tcatgacccg caatcccatt tatgtcatgg aagacgacct ggccgctgaa
    39901 gcggcgaaag ccgtgagcaa ccgccacatt gacgatctgg tggtgctcaa cgcggaaatg
    39961 gcccccgtgg gcatcattga cctccaggac cttgccaggc tgaaactggt ttaaaccctc
    40021 cctttcccat tctttccagg catccgtgat tcaaaagtaa caaatcaatc acggatgcct
    40081 ttttcattga acagaagctt ccgtttccac aatcttgttt tcataaacat gttcctgaaa
    40141 tttaccatcg ccctgcttgc ctgcacctgg ggcttttcct gcgcacagga catctccggc
    40201 ccggaggaag ataccaatgc ccccgctgcc gaggaagcca cgcgggaagt ccctcttccg
    40261 gacgggaaaa cggttcccaa atttgaaccc ctggatgagc accgcatcaa aatcggcaat
    40321 gtgattgtca accataagga gcgcaccgtc agttttcagg ctcaagtcaa catgaaggaa
    40381 ggaattctgg aatacgtctg ctgcatgccc aacggcaagc tgcatgaatc cctgctggtg
    40441 acggaggcgg atcccctgca catcagcctg ggaatgacgc tgctcaaatt ccaccgcttt
    40501 gaaaaattct tcccggtgcg ggatgaaaat tttgaatggc ttcccttcac ggaacccaag
    40561 ccggaggact acgcagactc ctacgtgcag attgtcatga cctatacgga aaacggcagg
    40621 gaacagaaga gcgatttttc cgacatcgtc gtgaactcac aaacgcggaa gggactgaat
    40681 cccagcgact ggctttacac caattccttc ttttacgaag gcgcctacca ggccagcctg
    40741 agcggagaag taatctccat cttcgccagc cgcacctccc ccatcaatta catcggagac
    40801 ttccatgacg gcgtcaacga tacaggctgg atcgtcaacc cccaaaaaaa cctgcctctg
    40861 ggcacaaacg tcactgtcac catttcccag aaaccggttc aacccaagca atagcatcct
    40921 atgttcaaat ccgtactttc actaacgctg gccgccgcac tgggcacagc ctccttcggg
    40981 cagaccaccc tgacggccat tcccggagaa gcggcagccg ccaaatacgc ctccattagc
    41041 caggaaattc tgcgtgccat tgaaaaaggg aacgaatacc tgaaaagcaa acaaaatccg
    41101 gaaggttact gggcccagcc gtcctaccct gccctgacag ccctggcggt caccgcctac
    41161 atgcgggacc ccgccaacca ggggaaaccc gtcccggaat acatccggaa ggggtatgac
    41221 ttcatcctga aatcccagaa ggaagacggc tccattttca accgcggcat gtcctcctac
    41281 aatactgccg tgtgcatgat ggccctgctg gccgccaaca aggaggaata tgcccccgcc
    41341 attctcaagg gccgcgccta cctcatcaag cagcagaacc actttgcccc ggacaaccct
    41401 tataacggcg gcatcggcta tggagacaag caggctcctc ccattgcaga cctctccaat
    41461 acctccctgg cgctggaagc catttactac tcccagaaac tcgccaaaga cggcaagtac
    41521 ggtgaacagc cggatctgga ctggaacgcc gccacggaat tcatcaaccg ttgccagcaa
    41581 aaccctgccg taaacaagga accctgggtt tccaatgaca aatcccagct cggcggcttc
    41641 gtgtaccgtc ccggcgtctc cagcgccagg gacaaaaaaa gcgccgcctt tgacaaagcg
    41701 gagcccccca aagcttacgg cagcatgacg tatgcgggca tgcagtccct tatttacgcc
    41761 aatgtggata aaaacgaccc ccgcgtcaag ctggccctca agtggctgga aaacagctac
    41821 agcctggacg aaaaccccgg catgggcgtc caggggcttt actactacta tcaggctatg
    41881 tccaaggcgc tcagcgccat gggaatagat accctgacgc tggaaaacgg ccgcaaggtg
    41941 gactggcggg atgaactggc caacaagctc atttccatcc agaagagcga cggttcctgg
    42001 gtgaacacca acaaccgctg gtgggaggcg gaccccgtcc tggtgacctc ctactgcgtg
    42061 ctggcgctgg aacagctcta ccactccatt ccccggtaac ggactgccat gctgatttcc
    42121 cgggccggaa ggggaggcaa tgacctcctt ttccggcctt ttccgtaccc ggaaatgaac
    42181 gccccatgaa atagaagggt cttttcttca atcacttcaa attcatggac accattgatt
    42241 ccattaccat tattgcagcg gcatggggcc tgctggttct ccggctgacg ctggctttta
    42301 tcctgtggcc acacgggacc cagaaagtgc tgggctggtt cgggggcgcg ggctgggagg
    42361 gcacctaccg ggccttcacg gaaaaaatgg gcattccccc ctttctggcc aaggtggcca
    42421 tgctgacgga atttttcgcc cctatctgcc tgatactggg cgtattcacc agaatagcgg
    42481 ctctcagcgt tatcatcctc atggccgtag ccataaccaa acacgtcaaa aacggctact
    42541 tttccaactg gtccggcaaa aaagccgggg aaggcgtaga attccacctg ctttatgcgg
    42601 gctccgcttt cgccctgttc ctgacaggcc ccggcccttg gtccgtggac gcctggtgca
    42661 tgaacatcgt gcatgtcatc ttcggttgac ggcggcagac agcacacgga agggcataga
    42721 acatactttc cgattctcaa acccgccgta ttctggtatt tatggaaagg ctttcttttg
    42781 cctcgttaac cctttccgcc atgaccagcc ataaacgctt aaaactccgg cagtgcacca
    42841 actgcgaatg cgtcattaac ggagtatggg aatgggtgga ggaatgcgcc cgcagcgcag
    42901 cggcggaaga acccgccatg cgcggtattc tggatgacgt ctgcctgtcc cgcaattccc
    42961 tggccacttc catctcggcg cggctggccc gcaagctctc ccgccgtgac atgccgcggg
    43021 agctgattga gccccttatc cgggaagcat tgacggaaga ccagaacatc ctcagccaaa
    43081 tcgcccagga cctgattgcc atcgtggacc gggatcccgc ctgccgtacg ccgctggaac
    43141 ccctgctttt ttacaaggga ttccacgcca tcaccacgta ccgcatctcc cactggctgt
    43201 ggcagcatga ccggacaatc atggcccttc agttccagag cttggccagt gaagtatttg
    43261 cggtggacat ccatccggcg gccaccatcg gctgcggcat cctgctggac cacgccacca
    43321 gtttcgtggt aggggaaacc gccatcatcc gggacaacgt ctccatcctg catgaagtca
    43381 cgctgggcgg cacgggcaag gaagaagggg accgccatcc catcgtggaa tccggagttc
    43441 ttatcggcgc gggggctaaa atactggggc gcgtcaccat tggcgcctgc gccaaaatcg
    43501 cagccagctc cgtcgttctg gaaaatgtcc ccccccatac cacggtagcg ggcgtacccg
    43561 ccgttatcgt caacgattcc attccggaca atcccgcgct ggacatgaac cagtgccttt
    43621 gcgactgaac cggcaacaga ttgggaagcc atcacttacg gcagaaaaca ggttgaatcc
    43681 gcagctccgc caaaatcagc ggaaaaggca agccttaaaa agaccggact tgatacaata
    43741 gtttcaaccc caactcgcct gtatttataa aatgctgatt ttcaatactt gaagcaaacc
    43801 cgaatcctga ttggccaatc tgacgggccg cgcctgttca ggcacggctt tcccccggaa
    43861 tccgggacgg ctccttcagc tgcccgtttc cttcaataac atacttgtag gaagtcagtt
    43921 cgcgcagagc catgggaccc cgggcatgaa acttgtccgt gccgatgcct atctccgccc
    43981 caaagccgaa ttcctccccg tcgctgaacc gcgtggagca attatggtaa acgcaggcgc
    44041 tgtccacccg gttcataaaa taatcgcgtg cccgtttatc ctccgtaatg atggaatcac
    44101 tgtggtggga cccgtagcgg ttaatgtgtt ccactgcatc ccggacgccg tccaccacct
    44161 tgacggacaa aatgaggtct tcatattccg tggaccaatc cctttcttcc gccggaacgg
    44221 cctcctgacc aaaaaacggg cggctccgtt cacacacgcg gcattccacg ccacgcatgc
    44281 gcatgcgttc cgccgccata ggcaaaaacc gctctgccgc ggcagcatcc accagcaggg
    44341 tttccgccgc gttgcaaacg ccgggcctct gcgttttggc atcgtccagc acgttcacgg
    44401 ccatctctaa atccgccgcc gcatccacat acacatggca aatgccgtcc agatgtttca
    44461 acacgggaac tttggagaac tccgtcacgg cgcggatcaa ccctttccct cccctgggaa
    44521 taattacatt caagcaggaa tccagctcgc acaactgccg cacctcatcc cggctgccgg
    44581 aatccagaag ccgcacggca tccgcgggaa cagcggattt ctccagcgcg ctgcgaagag
    44641 tctccgccaa cacccggttg gtccgcaggg actcgctccc gccgcgcaga atgacggcat
    44701 tcccggactt caggcacaag gcggcggcat cgcaggtgac ggtccctcgg ctctcataaa
    44761 taaagccaat gacccccagg ggaacgcgca cctggcggat acgcaggccg ttggggcgtg
    44821 tccattcccg gatgcactct cccacggggt cgggaagggc tgctacctgc tctatgccgc
    44881 aggccatgtg ttctacccgg tccggcgtca gcagaagcct gtccagaaac gcggaattcc
    44941 ggccactctt ccgggcctct tccatatcaa ggacattctc ccggataatt ttctctgcgg
    45001 cgttccgcac tcctgcggcc atcagccgca gggcctgagc cttctcttcc ggctccaggg
    45061 caagcatctc ccgtgaagcc gccagagcgg cccgccccgc cggttcaaga ggattttcca
    45121 tcttcatgac ttcctccttt cggcacaaaa tacgttccgg ctccttcctc ccttccctcc
    45181 agcagcagcg caatgcgttc cggatggcgg ccatgcagca tgtaaacgcc cgttcccgca
    45241 ttcacggctt cccgtatggc ctgcaatttg gcgcccatgc ctcccatgga aaagcggccc
    45301 cggtcctcct cagcgaatgc cagtacggca tccacatcct ccacccggga aatgatggcc
    45361 tcccgccttc ctccgggggg atacaggccg tccacgctcg tcaggataaa cagagcgtcg
    45421 gcctccacca ggcgggccat atgcacggac aggatatcgt tgtccccaaa cttcagctcc
    45481 tccacggata cggtatcatt ctcattgaca atcgggataa tgcctccgtg ctcaaatagg
    45541 cgcaacaccg tggcctgcac gttgcggcgg cggagtttca aatcctccgc cgtcagcagg
    45601 agctgggcca catccacgtt aaagtggttg aaaagatttt catatgcctg catcagccgc
    45661 gtctgcccca cggccgcgca cgcctgtttc aaggacagtt cctgaggata ggacggaagc
    45721 cccaggacgg acacgccggc ccctacagcg ccggagctga ccagaaccac ctggtggccc
    45781 tgctgcatga gtcccgccag gacgctcacc aaatgcacaa tggaagaacc gtccaatgtc
    45841 ccattctcac gggttaacac gcccgtacct actttaatta cgattttcat gtatctccgc
    45901 cgttcatgat acaacaatcc ctttttccac agaaagcaga aaacgcgtcc tttccgcgct
    45961 accccgtgaa agcgccggcc gtaaaaaatg gaaggaattc ccccttctta ctccataagg
    46021 ataaaaaaga taattccctc cgcccgaccg gctccggcgc ggataaaatc cgccatcccg
    46081 gccaatatct ggacagccat gaaaaccagg ggaggacaac accggggaaa aggactggag
    46141 caggccgtgc gacatgtttc cccccggctt tattccctaa agctccatgc atgccggtga
    46201 gcgtgccgca aaaccgggaa cggcatgcgc ggcgcttcca tcttcaatgc ttttccccgg
    46261 tccggaactg gtgccggaga cgcattttag gcttccaata tgaagctgat ccgctcatat
    46321 tgcccgggca tgaatcttga ccttctccaa aaagccgcca accaggcgcg gggactcgcc
    46381 atggatgccg ttcacgactg cgcatccggc cacctgggcc tgccgctggg gtgcgccgaa
    46441 atcggggccg tcctgttcgg cgatttgctc aacatctgcc cttcgcagcc ccgctggctc
    46501 aaccgtgacc gcttcattct ttccgccgga cacggctcca tgtttcttta cggctggctc
    46561 cacctgtcag gattcaacat cggcattgaa gatatcaaga atttccgccg caaaggttcc
    46621 atcacgcccg gccacccgga attccgggat acggagggtg tagaatgcac cacgggaccg
    46681 ttgggccagg gcattgcgaa cgccgtaggc ttcgccctct ccgccaggcg cgcggctgcc
    46741 cggttcaaca ggcccggcat ggatatcttc acccagcacg tcttctgcct cacgggagac
    46801 ggctgcctgc aggaaggcgt cgcccgggaa tccctcgccc tggccgccgt gctcaagctg
    46861 gataacctta ttctcattta cgattccaac gacatcacgc tggacgctcc cgcggaacgc
    46921 acccagctca cagatccgcg cgccgtatac gaagccctgg gctgggatgt gcgccagatt
    46981 gacgggcacg atatcagggc cattgagtca gcggttgaag ccgccaaaaa cgccaaaaac
    47041 gggaaacccc agctcatcat tgccaaaacg gtcatcggca agggcattcc cggcattgaa
    47101 ggcaccacaa aaggccatgg ggaaggcgga gccaagcttc aggaagaagc gcacgccaac
    47161 tggggaattc ccgccggaga acgctattac gtctccgaag acgtccgtac cgctttcgca
    47221 aacctgaaag cccaacggga aaaagatttc aatgcctgga acgccatgta cgaacaatgg
    47281 cgccgggctt atccggaact ggcagaggaa ctggacgcag gcatcaacgc ctgctcctgc
    47341 ggcgtcaatc cggcagactc ggacaaggca ataccgccct tcccgcagga ctatggcgat
    47401 gctacccgtt cggccggagc cgttgccatc aacgccatcg ctaaagcgaa tccctgcttc
    47461 ctgaccacca gcgcagacct ttacagctcc aataaaaact acctttccgg cgcaggagac
    47521 ttctctgcgg aaaccccgga agggcgcaac ttctggttcg gcatccgcga acacgccatg
    47581 gccgccatct gcaacggcat tgcctacgac ggcctgttcc gcgtaagcgc cgccactttc
    47641 tgcgtcttcg tagactacat gcgcgcctcc atccgtgtag ccgccctcag cggactcccc
    47701 gtcacctata ttctgacgca tgactccgta gccgtgggag aagacggccc cacccaccag
    47761 ccggtggaaa caatttccgg cctccgtgtc attccgaacc tggatgtcat ccgcccggcg
    47821 gacccggaag aaaccgcagg agcctggatg gccgccatgc agcgtgccga cggccctacc
    47881 gccctcatcc tgacccgtca gaaagtggct acgctgaacg gaatccccgt tgaaacgcgc
    47941 cgggaaggcg tgctgaaagg cgcctacatc gcccggaaag aacagggagc cttgaaagcc
    48001 attattcttg ccagcggttc cgaactggaa ctggctctga aagcggcgga aaaaacaggg
    48061 gaaggaatcc gggtcgtctc catgccaagc ttctgccgct ttgacgcgca gcctgccgaa
    48121 taccgggaaa gcgtgcttcc ctcctcctgc atgaggagag tttccgtaga agccggagtc
    48181 acggacctct ggtggaaata tctgggctgc cagggggaag ccgtgggcat caaccgtttc
    48241 ggcttctccg ctcccggaac acaggtgctg gaagaactcg gcatgaatgt ggacaacgtc
    48301 gttgccgccg tccacaaggt tctggccaaa taaacaggga atatttcccc tcatctcctt
    48361 tcaggggctg ttccgcatcc gccgggacag cccctttttc atgcctcccc ggttgccagg
    48421 aaaaaggcaa ggccgttttc acgaaacacc agtatccgta acgcaggaac tatatccttt
    48481 cctgtgaaaa ttccgttcgt ttcccgtccg gatttctcta aatacaagac ctccgcacat
    48541 cagctccatt gtccgaaccg gacccggatg ggactcaggc cgcaaaagcc ccggttgcaa
    48601 gcagcccgtt ttcatctcag ctgccggaac ggataatttc aggtttcctt catggcgccg
    48661 ataaatatgc ggggcggcca tgatcaagcg ccgcctctat cgacgatgcg atgcacccta
    48721 aacccgcaaa ataacgaggg tccggcccat caacaatgaa aaaccgcccc tttccgaaaa
    48781 aaggggcggt tttaaagacc ttggcgacaa gggaagcggc tcctttagaa agcggcttca
    48841 atcttcgtaa tagtatacac cttttttttg tcgccgtcca tcgggacaat cttcacctgg
    48901 tcccctacgc ttttgcccgt cagctccttg ccgacctggg aaagatagga gacgatatgc
    48961 ttttccggat ttgtgtccca cgcgcccaga atggtgtaca ccacgggctc cccgccattc
    49021 aacggctgaa gagtcacctt cgttcccatg gcggcgcggg aggtatccgt cacggcaaag
    49081 tccgtgccgc gggccagggc cagcgcatgt tccagttccg cccggcggcg gttgagcacc
    49141 ttctccactt ccttggcatc ctggtagccg ccgttttcgc ggagatcccc ctccgcacgg
    49201 gaaatcttct tgttatgcag gttttccgga attttcacgt ttaccagttc ttccagctcc
    49261 ttcttgcggg cctccaggct ttcccaggaa acgaaaatcg gttccggttt gtcggagttg
    49321 tccaccaggg cgtccagcac aatggcgtgc aggtccggga atacgctgat gatgcgggcc
    49381 atcagcgcgc cgcggtcctg ttcggagaaa gcggaggtgt tgtacagcat cttggcaaac
    49441 tggcggactt ccgccacatc ctgccccttg atcatttcct ggatgatgct cttatcctcc
    49501 atgaacaggt tcttcaggcg cagcatgcgg ttggggccgc cgtccatgtg gtcctgttca
    49561 atcagggaaa gcatcgcaat gcccacaggg aggccaaaaa gaggcttgga agcgtcatgc
    49621 cgctggcggc atatccagat gagggactcc gccggaaggg tctggcgcag caattcatgc
    49681 ttcaaatgct tgaccagtac cttgtcctgc ccttcttcta tcaggaattt ggcgatttcc
    49741 cccacggcgc gggcgccgcc tgcatcaaaa agctccagcg ccttctgcgg ccaatcctcc
    49801 ccaaagatgg cgggcagcgt ggcatacacg cgcttctgcc gcacggcagg catggaattg
    49861 agcacttccg cgaagcggcc cacatcgccg atattggctt ccacgatgga acgaagggga
    49921 taagcttcct tggccgcctc cgtattttta agggaggcaa tcaggtcatc cctgagcacg
    49981 gccagctcca gcacctgctg gagagccagg ctgccgccgt tgcgcacatc agcatccacg
    50041 gcacgaatca gggcattgac ggcatccggc tctgccatga ccgcttccat tttgatgccg
    50101 tccagcacac gcaccttggc tttcaggtcg gcggccttat tgtaatcttc cagggccgcc
    50161 tgcgcgcggg aaaggatatt cgcgcgaagc gtgatgcgtt cgcccttgcg ggtgggcatc
    50221 atgaactgga cattggaacg gagcatggcg cgcaccttgt cccaccagtt tttccacttg
    50281 tccgccgcaa ttacggtgcc tttcagcgcg gcttccagct tctccggagt aagggaatta
    50341 ccgtagccct gcaaggtaac gcgcaccagt tccaccgggt catccacgga caggttgatc
    50401 agggattcgg tatcttcata ccgcttggcc aggaaatggt cgtcactgat gaactccaaa
    50461 attctgggag cgaattccag ggcaacttcg tgttccggat tttcctcaaa atcaatgacc
    50521 agctttttgg cggggagatt ccaggaaatg acttttccgg cgccccagga agcatggaag
    50581 cagtaacggc caggtgcgat gcgatcaagt ttttcggcga gagagggggt aattttcccc
    50641 agagacaata atttctccaa gtcggcgtgc atactaaagc acatgatacg ccccccccta
    50701 ccggtatcaa gactaatttc cctcttccgt ccggaggcat cccgaacggc tttccaaagc
    50761 cgccacggag acagcgtccg tccgcgtcat gctccgactt cgcattcggg tttcgcttct
    50821 ttttccctcc cctccttcat ccagttgcgg cttgaccgtg cggagcgtct ggcgtaacat
    50881 ctccgcacgc attcaacccc tgcgcaacac gtatcatgga acctatttac gaagggaagg
    50941 ccaaacgcct ttatacgact gacaatccca acgtcctccg catggaatac aaggacgacg
    51001 ccactgccgg aaacggagcc aagaaggccc agtttgaaaa caagggcagg atgaataagg
    51061 ccatcaccct ggtcatctac cgcatgctgg aaagcaaggg cgtgaaaacc cacctggttg
    51121 cggacgtgga cgacatcaac attgacgtaa aaaaggtttc catccttcct ctggaagtga
    51181 tcgtgcgcaa catcgccgcc ggctccttca gccgccgcat gggcgtcaag gaaggcaccc
    51241 ccttcaagaa gccgatcgtg gaattttcct acaaggacga cagcctgggc gaccccttca
    51301 ttaatgatga ctacgcccgg gaaatgaacg ccgctacgga agaggaatgc gtcttcctga
    51361 agaatcaggc ccgcatcgtg aatgacgtgc tgattgactt tttcaaacag gtcggcctta
    51421 cgctggtgga cttcaaaatc gagttcggcc gcttggcgga agatccctcc caaatcgtgc
    51481 tggcagatga aatttccccg gatacctgcc gcctgtggga catcaaaacc ggcgagaaga
    51541 tggacaagga ccgcttccgc caggacctcg gcaacgtgat ggaagcctac gaagaagtcc
    51601 tttcccggct ccagaacaag gcttgagcct cttccccctt cccatgggag ccggtcttcc
    51661 gccggccccc atttttcatt ttaccacact cttgagcgat atgaccctcc actttgaagt
    51721 tatcagccgg ttccaggccg ccgccaatgc ggacgccctg ctctataccc ccctgaacgc
    51781 cggcctcact ttccgccgca gccgcgttta caccgtagaa atcaccgggg acgaacaaaa
    51841 agcccgcgaa tacctcctga gtgtgctggt ggaccccatt tcccaggaat gcacggaaca
    51901 gaacgctccc atcctgaaag gcgccctgtt cacgattgac tacggcatga aagccggcgc
    51961 cctggatctg gaaaaagaag ccatcctgca aaactaccgc gggcgccgga atatgggctt
    52021 tactatggac ggcctgaaaa tcacccagcg cgtgtacgtc ttcggccagg gagacaagga
    52081 aaccctggcg gcgcgctttg ccaaggacgt ctgcaacccc gccattcaca actggaccat
    52141 cgcctaaccc ttttcctttc ccatccacat gtccgccaaa acctaccgaa ccgttcccgt
    52201 gaggaacctg agcagcgacg aactgcttga gctcagcaag aaacagaagc tttccctgtc
    52261 ccgtgaagac atggaagtcg tccagcagat tttccgggaa atcgaccgcg accccactga
    52321 cgtggaactg gaagtaatcg cccagacgtg gtcggaacac tgcaaacacc gcatcttctc
    52381 cgcctccatc agccacagcg tcaacggcgg cgcaccggag acggtgaaca gcctgttcaa
    52441 gacctacatc aagaagcctt ccgaaaagat catggaacgc aaaccgggct tcgtgctcag
    52501 cgcgtttgac gacaatgccg gcttcatcgc cctggacgac aacctggccg tctgcctgaa
    52561 ggcggaaacg cacaaccacc cctccgccat tgaaccgtac gcgggggcca acaccgggct
    52621 gggcggcgtt atccgcgaca tcctgggcgc aggcaagggt gccaagccca tcgcctccct
    52681 ggatgtcttc tgcttcggcg ctccggacac gaatccggca gacatcaccg ccccggacgt
    52741 catccacccc ctgggcatca tgcgcggcgt ggtgcgcggc gtgcgcgact acggcaaccg
    52801 catgggcatt cccaccgtca acggcgccat ccagtttgac cccacgtaca tttacaaccc
    52861 cctcgtcttc tgcggcaccg ccggcgtgat tccgcgcggc gacatcctga aggaaatgcg
    52921 tcccggcctc aaggtcatcg tcatcggcgg gcgcacggga cgggacggcc tcaagggagc
    52981 cacgttctct tccgccgctc tggatgaagc ctcccatgag gaagacttca ccgccgtgca
    53041 aatcggtaac ccgattgaag agaagaaaac gctggacttc attctggaag cgcgcgaacg
    53101 cggcctcatc gtctttatta cggactgcgg cgccggcggc ttctcttccg ccgccgggga
    53161 aatgctctcc gtcacgggcg gtgaaatttt cctggacaac gcccctctca aggaaccggg
    53221 cctcatctcc tgggaaatct tcctgtcgga atcccaggaa cgcatggtta tggccgtgga
    53281 ggaaaaaaac cttccggaac tccggaagct ggcagacacc ttccagacgg aactgaccgt
    53341 cctgggccat tccgacgata cgggaatcct gaaggtctgg cacaaggggg aactcgtctg
    53401 ctgcctggac aactccaagc tgcatgacgc ccccatcaag aagctggaat ccgtattcac
    53461 tcccggcaag gcgctcacgg gccagcccct cccggacaag gatctgaaca aatccctgga
    53521 aaccgttatg ggggatttcg ccatcgtttc ccgggaaccc atcatccgcg agtatgacca
    53581 cgaggtgcag ggcaacacca tcctgaagcc cctagcgggc gcccaggcgg acgctccgca
    53641 ggacggctcc gtggtggaca tcgacggctc ggacaaatgc atggccatgg cttgcgccat
    53701 tcttccggaa tggggcaaaa cggatcccta cgccatgggt accggcaccg tggatgaatg
    53761 tgtgcgccag ctcatcctgg ttggctccaa cccggacaaa atcggcctgc tggacaactt
    53821 ctgcatgggc aatccggaag atccggcgga actcggccgc ctggtggaat gcgtgaaagc
    53881 catcgccaag gcagcggacg cgtacaatgc ccccttcatt tccggcaaag actccttcta
    53941 caactatttt gaaacggaag acggccctat caacgtgccc gtcaccttcc tctgctccgg
    54001 ctttggtgtg gtggaaaacc cggaccacgt catcggttct tccctgaggc ggagcaacag
    54061 cctgctgtac ctcatcggcc atacggaaga tgaaatgggc ggttccgtct tcgcccgcac
    54121 ccacggggtg gaagacggca aggttcccca gacggactgc gccgcgaaca tggaactgta
    54181 caaagcctac tacggcgccc tgacttccgg tctggtgctc tccgcccatg acgtttccga
    54241 aggcggcctg gccgtcacgg cggcggaaat ggccttttcc ggcaagggag gcgttcggct
    54301 ggatctcacg aaagtaccga ccgtcaacgg ctggaaatcc cccgcagtgc ctctgttcag
    54361 tgaaagcacg ggccgcatcc ttgtggaggt ggatccggaa ttcgccgcgg attttgaaac
    54421 ggctatggac ggcttcccct gcgcctgcat cggcggtgtt acggaggaaa agcgtctcac
    54481 ggccacctgc tgcggcggag aaaaggtgct ggattgcgac atagccaacc tcaaaaaact
    54541 ctggaaggac ggacttaccc cctattattg acctcctgaa accggcatct tatcaaacca
    54601 tcgatttatt tctaccaata tgcctcacgc acttttactg aaataccccg gcaccaactg
    54661 cgatgtggaa acggaacgcg ccctgcgcgc ggcagggttc tccacccaga tccagcccat
    54721 cgccacggct acgccggaac acgtcgcaaa atcccagctt gtcgtcctgg gcggcggctt
    54781 cagctacgga gactacgtca ccagcggccg cctggcccag ttggaaacgg aacgcttcct
    54841 gggagatgcc ctgcagcggc accatgccgg aggaggcatg ctcctgggca tctgcaacgg
    54901 gttccagatt ctcaccaagc tgggcattct tcccgactcc agcctgattg acaacaaaaa
    54961 ggaacgcttt gaatgcgtct gggccaaact ggtgaaagta gccaggaact ctcccttcct
    55021 tcaaggcctg ccggatgaat tcgaactgcc ctccgcccat gcggaagggc gcctggtcac
    55081 ccgtcccgga gatgcggaaa aatacatggc ttccgggaat gtggctctcc agtacgtaga
    55141 caaccgcgac ggctgcgaat gcagtatcgc cggcctccag gattccaccg ggcgcgccat
    55201 gggtctgatg ccccatccgg aacgctttct gctgccccgc caccactatg acaaggactg
    55261 ggccggcaac gaagactggg gctgggggta cttcctcttc aaatccgcct ttgacgccct
    55321 gaaataaagg tccatttccc attaaccgct ccccccgcat tcattccatg acagatacgg
    55381 ccctcatcgg ttcttcttgc gccctcggcg ttttctggtc ctcaaaacgg tactttgact
    55441 acctgattga tgaaaccaac ctgtccctgc tgcgcggcaa ggaattcggg caggccgtca
    55501 ttatctgtcc ctccctccgg gaagggggca cggccattcg ggggaatacg gctccctttc
    55561 tggctcaggt taaaaccctg attgatctcc tgcatgaggt gaaggcggaa cgggtgacct
    55621 acatcaccag cattgacacc caaccggaaa cgggaaatga actttctccc ttgctccgag
    55681 aaacggagga cgaatggctg gcggctctgg tagagctgaa agacttcatc aacctccggt
    55741 tcggccgcgt gctgaacgtt taccttccgg aagtcaccgg aacgggagca ggcatgagtg
    55801 tggcggatct gctggcagca gttccggaag ggacggaaga gctggacgta gccctgctgg
    55861 aacgccacca gctttatccc atgcaccgcc tggtcaggga tgtggaaaaa gcatgggagt
    55921 gcggcatctt ttccgtgaat ctggtgccgg aacccgtcac ggcctttgaa ctggtggagc
    55981 agtgcttccc ttccctggtc aaccgcctcc ccgtcgcgaa ggaaacggac ccgtacggga
    56041 gcgccagaac atccgtttac tccacgcagt accacgatcc ggacaccggt tatatcatgg
    56101 acaagcagga cgtcctggcg gggctctctc cggacaaaca gtcctagccc ccggtcagcc
    56161 cccgtctgcg ccctgtgcag tcggggcttt ttatccccca ccttcaaaaa agctcccccg
    56221 caggagccgt gctttcctgt tgagcgcatt ccggaacggt gctatacaaa agccatgaag
    56281 attagcgacg acagccttga attcttaacg gaacttctgg aaacccccag cccttccggc
    56341 tttgaaatag atgcccagcg catctgggcg gacgaactgc gcaaatatac ggaagacgtc
    56401 cagtgcgaca cttacggcaa tacctgggcc gtcttccacg cggacgcgga agaagccccc
    56461 acattgatga ttgaagccca cgcggatgaa atcggcttca tgatccgcca tatcaccaag
    56521 gacggcttcc tgtatgtgga acgcgtaggc ggcacggata cggccatcgc acgggggcgc
    56581 cgcgtgcgct tcctgggttc ccagggagaa gtgatggggg tgaccggaaa cacggccatc
    56641 cacttgcggg aacccggaga gaaggaaccc aaaatctggg aaatttacgt tgatgtaggc
    56701 gcctcctccg acaaggaagt agcggaactc ggtttgcgcg tgggccatgt aggcgtttac
    56761 tgcgacggcc ccatgctgat gaatgaaaac aagctggtat gccgggctct ggacaaccgg
    56821 ctgagcggct tcatcctgtc ggaaatagcc cgcaagctgt gcaagctgaa aaagcccgtc
    56881 tcctggaacg tggtgctcgt caatgccgtg caggaagaag tgggctgcat tggcgcggga
    56941 atgattaccc accgcctgcg cccggatgcg gctatctgca tagacgtgac tcatgccacg
    57001 gactcgcccg gactggacaa gggcaaattt ggcgatatca ggcttggcgg cggccctgcg
    57061 gtcatccacg gcacggccaa ccatcccaat ctggtggccc gtctggaaat cgtggcggac
    57121 aagaacaaaa tacccctcca gcatgaagcc gccggacgcc gcaccggaac ggatacggac
    57181 agtatctaca tctcccgcga cggcgtagcc tccgcgctgg tgtccgtccc cctgcgctat
    57241 atgcactccc cggtggaaac ggcctctctg acagatgtgg aaaatacaat caagctgctg
    57301 ctggaattgg tcaaatccct gatgccggga gactctttcg ggcacaagct gtaatacggc
    57361 acggacggca gcattgctga caaaatgagg acatgaaccg tcctgccgga agaaggcgcg
    57421 caaaggcgtc aatttccccc ggcacgcccc catgcggcag cattcaggcc gcatctcccc
    57481 tttgaagcag gggtggaatt actccctgca ggcatcctgc gtcctcatac ggtcattctc
    57541 cgcttcccgg cggtgcttcc tgttttccgg cccctggctt ccttttccgg aatattttcc
    57601 cggaaaccat tacaggccgg aaaccggccg gaactctttc tcccggtcac atttcactga
    57661 aatccagccg ggtcacatta aagtacgttt ttcccttccg gcaggaatcc cacaggagaa
    57721 tcagcatgag gaaaaagagg accagtctcc aagccacttc aaagggatag ctggttacca
    57781 ccctctccac accaatgcag ttgcaggaga gggaaagccc cctggcccag ccctggacga
    57841 acaacgccac gaacgtgaac gtaagcgcac tgcccagcag cgcggccccg gcgcaggttc
    57901 tgcggaacag aagccccagg ccaaccgcaa tctcgcacga cactcccagg caggccagag
    57961 gatgccccca ggcctccggg aaaatggcga aacggtcgat cgtctcctga agctggtcca
    58021 gatgaagcag cttgcgcgcc gcgacaaaaa ggaaaaagcc ccccatcccc agccggagca
    58081 ggagcaaaca gaaacggacg attttttcct ttagcatcat tggcgtattc cgattattcg
    58141 aagtgggttt gctgggcgct cactttttca ttgcgcaacc tgttcagttc atggcgcact
    58201 ttttccagct ccatctgaag ggatttctcc cgttcggagg caggagcctc ggaggaagac
    58261 ggcgtaccgg caaccttctt cgcgctgcct gcggcgtctt ccgtaaagag cattttcaac
    58321 ccgggagcct tcgtgtcaaa ccttatgacg ccggaaacgg gcttttgaga accgggatgc
    58381 ttcacggagg ctctcaccgt caccgtgccg ggtgttgccc ccaggcgcac cagggccgga
    58441 gcggaacccc atttaacgga ctgggtcagc gtgccgtccg gggaatccgt cagcagctcg
    58501 gcggggcctt ccacggaaaa gacaatctcc tcatcattca ggcgcttcac tgttccgcgg
    58561 ccatccgtaa cctccgccac cacggcaaac acatcggaac cgttggcgta caaatccact
    58621 ccatcttccc ggtccagagt caaccggatt ttctccgccc tccgggccgg gcggacttca
    58681 tgcctggtca ccaccttgcc gcctatcagg ccttccgcca ccagtttcac cgctccctcc
    58741 ttgcctgctc gggcaagctt cttgttttcc atgaaatccc aggctttagg gaacacgacg
    58801 ggcacgcgct tgaggcctcc gggacaggac gccaccctct tttccaccgg gggccctccg
    58861 ttgacactca ggcgaacgga atcacaattg gaaaacaccg tcacgtcttc cggagaaaag
    58921 ggagtgcatt cattggcgat gtgcaccaca gggccggaac ccaacccgtt gcgcgtcttc
    58981 tggggacgct gggacataaa tgcgtaatag gaggttttgg gctgcctgaa agcatccaga
    59041 ataccgccgt agaagggatc cggatggtaa ccgcgctgat ggtcgaaagg atgccacagg
    59101 cagccgccga aatgataggc cggagcggcg ttcagcgtat cgtacgtcgt atatgggaac
    59161 gaggtcttga ggtagtgaag ggcctgcacc atcatgggag cttcacccca gtgacgggct
    59221 acacgggagg tggagttgtg ggcggaccag gtatccacgt tatctccgaa ttcacgggta
    59281 aaatacggtt tgctgttctt ccgttccttg atggaccagt gtttgtcccc cgtaaccggg
    59341 tgggcataca gaacttcata atgctggctg ccctgagcca ccgcgtcgca agcggtgtag
    59401 catcccttgt acggatattc ctcatgcacc aggtcgcggg cctttttggc gaaatccgcc
    59461 gggtaatgag tttcattcaa aataggttcc cacatcatca cggagggatg gttcctgtcc
    59521 cggcgcacca tctggcggat atcgccatac acccgatccg caaaagggcc gctgccccag
    59581 aactgccagc ccggcgtggc gacgatgacg aaaaggccca gacggtcgca ggcgtccatg
    59641 aaagccggat cctgcggata atgggcgctc cggatcacgc gcatgcctgc cttcctcaat
    59701 ttcacggcat cctgccattg caggttgttg ggcactgcat tcccaagccg ggcaaaatcc
    59761 tggtgcctgt tgccgccaat cagcttttca ggaaacaggg atccattgag gaccagcccc
    59821 ttttccgtaa tttccaccgt ccggacaccc acccttatgg aacggttgtc cagcaaatgg
    59881 gccggatctt tggaatggtc ccctccagca gcgtcccata cttccacgct caacgtgtac
    59941 aaatcgggat tgtccggaga ccacggtttc acgttggaaa gcacaagagg aatatccact
    60001 tcacccgtgg aatggggcgg aatggtcagc ggaaccgtct ccccggcagg atccaccccc
    60061 ttggggtccg tcatcaccgc ctgcacgcgc accttccgtt ccttgtctgt ctggttggcc
    60121 acctgaacct tcacccccac cttgcccttg cgggtgcggg gatcaagaga ttccgtccgg
    60181 aaaaaaactc cccccccggc cacggttccg gcctcgttcg ggtccgtgat atacacgggt
    60241 ccggtggaaa tgaggtacac atcccggtaa ataccgccaa aataggaaaa atccaggccc
    60301 tcctggggtt tgccgggggg ataggaagcg tcgttggaat tgtccgcctt cacggcgatg
    60361 acattctttt gtcccggttt cagccacttg tccagattga caatcacggg aagatatccg
    60421 ccgaaatgct ccgcggcctt ctccccattc acccagacct cgctctttcc catgatgccc
    60481 tcaaaataca gggtgtttct cctcccctcc agccgggagg gaggaataaa tgttttccgg
    60541 taccataccg gcccctggta gttggaacag ccgctggcct cttccggaag aagctcaatg
    60601 ccgttgggga ggttgacgga ctcccaggag gaatcgtcaa catccgggct ggccgcttcc
    60661 tccggcgcct tctctccctt gaacagtttc cagcccacat tcatgccgta aacgtccctt
    60721 ttcgttacgt tttccggcag gcgaagagcc cctgccacgg aataggctcc cgtagatgaa
    60781 gcggcctcct cctgatcgga aactgcatcg gcataaaggg aaaaagagcc ggccagacat
    60841 gccgccgtta tgagaatggt agtgactttc atcatggaat aggggaatgc aaaattaata
    60901 tacggagctc ccccggcgcc atctttcatt catataatac atccttgcgt ataatgcaaa
    60961 tacatatgtc atccggcatc ttccgcttga cctggaaggc atgttttttc atagtcgact
    61021 cgccctgtac gggattatcc cgcagccccg gtccgagtgc caggttttca gtcccgtgaa
    61081 aaggaaaaag cgaggcgttc ctccaccggc tgtctcaggg ctatgccttt atccactagt
    61141 tttttatgtc atctttcttt cagaaaattg tcaaactttt ctcctatgac attggcattg
    61201 acttgggcac cgccaacacc ctggtcaacg tcaaggacca gggcatcgtt ctgcgcgagc
    61261 cttcggtcgt cgccgtcaag ggggacaagg ttctggccgt gggagacgaa gccaagcgca
    61321 tgctgggccg cacccccggc tccgtcacgg ccatccggcc gttgaaggac ggcgtcatcg
    61381 ccgacttcta catcacggag gaaatgctcc gctacttcat caaaaaagcc acggccaggt
    61441 actacctgat caagccgcgc gtgctcatcg ccatcccctc cggcattacg gaagtggaac
    61501 gcagggccgt caaggaatcc gcggaagcgg ccggagcccg ccgcgtgcat ctgattgaag
    61561 aacccatggc cgccgccatc ggtgtgggcc ttccggttca ggaagctgcg ggcaacatga
    61621 ttgtggacat cggcggcggc acgacggaag tggcgcttat ttccctttcc ggcatcgttt
    61681 acagccgttc cgtccgctgc gccggggatg aactggacga cgccatcatc tcctacatga
    61741 ggagaaccta caatctcatg gtcggggaac gtactgccga agacatcaaa atccgtatcg
    61801 gctccgccta tcccctgcct caggaactct ctatcgaggt caagggccgc gacctggtcg
    61861 cgggcctgcc caagacagtg acgattcgat cggaagaagt gcgtgaagcg ctcacggaac
    61921 agctcaacac aattgttgac gccgtgcgga cgactttgga acgctgcccc cctgaactgg
    61981 ccgccgacct tgtggaccgg gggattgttc tggccggagg aggcgctctg ctgcgcgggc
    62041 tggaccagct cctgcatgac cagacgggac tccccatcca cattgcggaa gaccctctga
    62101 gcgccgttgc ggaaggtacc gggaaagccc tccaggaact ggacttcttc accaatgtta
    62161 ctgatgcgga agactaaaga cggaatctcc ggagcaagcg ctccggcctt gcgtctgccg
    62221 tactgccgca tcacatcaac gcaactgcct gcatgattcc tttccggcca tccctacagc
    62281 ttcaactcca gccgctcctg acgggagacg gcccaccgga aacattacgg gcaatagcac
    62341 accctagctt attctcaccc cgtgtttaca aaaatgctta aaaaaatcaa acgggccttc
    62401 ggcttcggat cctgcgatcc catggaaggc tccactatca tcatcaatgc cgaaaagctc
    62461 gaacgccgcg tggcccttct ggaaaacggc gtattggaag aatacaccat cgagcgcgaa
    62521 ggcgaagaca acatcgtagg cggcattttc aaaggccgcg tcaagaacat tgagccgggc
    62581 ctcaaagcca tgttcgtgga catcgggcag gaaaaaaacg cctttctgca cttctgggac
    62641 gcgcttcccg ccgcactgga ctccagcctg gaagaaatcg aacgcgaagg ccgtccgaaa
    62701 aaacagcagc gcaaaatcac gtccaaagac atcccggaca tctatcccat cggctctgaa
    62761 atcatggtgc aggtcaccaa aggaccgatc ggcaccaagg ggccccgtgt cacaacgaac
    62821 gtatccctgg cgggacgcta cctggtcctg atgcccttta cggaccagtt cggcatctcc
    62881 cgtaaaattg aagaccccaa agagcgcctg cgcctgcgcc agatcatgca gaaactgaat
    62941 gttccggatg gaatgggcat catcatgcgc acggtggccc agggccagcg ctaccgccac
    63001 tttgtccgcg acctcgccat gctgctggaa caatggtaca gcgtggaaga aaagaaggag
    63061 gccaatccgg cccccgtctg cgtgtacaag gaaccggacc tgattgaacg cacggcgcgg
    63121 gatttcctta cggacaccgt ggacaacatc atctgcgaca acgcggaaac cacggaacac
    63181 atgcgcgcca ttgccggaaa aatctcgcgc cgcgccaagc gccacatcca gcacttcccc
    63241 gtgcgcacgc ctatctttga agaactggga attgaaaaac aaatcaatga agctttccag
    63301 cgccaggtgc tcctgccctg cggaggctac ctggtgattg acgaaacgga ggcgctgatc
    63361 gccattgacg tcaacaccgg acgcaacaag ggcaccaagg atctggacaa gaccattctg
    63421 gataccaatc tggaagccgc ctatgaaatc gcccgccagc ttcgcctgcg caacatcggc
    63481 ggtctggtgg tggtggactt tatcgacatg cgccacagga aggaccagca ggccgtttat
    63541 aaggccatga aggaccgcct gaaacgggac aaggcaaaaa cccaggtact gcagatcacc
    63601 cccatcggcc tgatggaaat gacgcgccag cggctcaacg agagcctccg ggaatccgtt
    63661 catgatccgt gcccgtactg cggaggcagg ggccgcgtca aatccgtgat gaccatgagc
    63721 gtggaaattc agcggcaaat ttacgcctgc atccagaaat accgggacac catcggagac
    63781 atggtcatcg tcgtcaactc ggaagtgctc tcccgcttca agaatgaaga cgccagcctg
    63841 ctcatggaac tggaacgcca gcacagcgga aggctgattt tccggggcga ccacaaccag
    63901 caccgcgaat ccttcgccat ttacgacgcc cggtacgaca acaaacccct ctaccagtcc
    63961 gggcaatagc ctgaaaatca aatccggctt tccgggccgg gcgataccct cgcccggcct
    64021 tttttacgcc gccatgaaca acatccccac ttcccccaac gaccccgtca acacggacat
    64081 tctgctggtg gcggaagacc gcatcaaagg gttccgccgc ctgcccttcc acgccatttc
    64141 cgaacaatgc ggacatcctg ttccggtggt tatccagcgg ctgaaagcca tgatggaggc
    64201 cggagtagtg cgccgcgtgc gccagacgct gctggcgacc aagctggccc agggagccct
    64261 ggtggcctgg tccctgccgg aggaaaaact ggatgccgct tttgactgga tgaaggagct
    64321 ggaccctttt acgggccatg tggtgctccg caatacggat gcggccaacc ccggcgccaa
    64381 ctacaggctc tggaccacgg tcaaggtgcc cgtggggtgc ggtacgccgg atgaacactg
    64441 cggcatcctg aaacgccata tcggcgcaga cggttatgtt ctgctgccgg cacgcggcgt
    64501 ctttgtgctg ggggtggggc acatgcgccg caggggactc aggcccggag aaaaaactcc
    64561 ggaaccggct cccatgcagg aaacgaaaac cgtctctctc tcagaactgg aatggaaggt
    64621 gctcctctcc gtcaaggaac agctcaggcc tgaggaaatg gtggaagatc cctggtccct
    64681 ccgtgccgcc cgggcaggat tggaaacgga agaatactgc cgtacggcgg aagaactgga
    64741 ccggaagcag gtcatcggcc gtttcgccac ttttctggaa catgtgagag ccaaaacgga
    64801 cgacggccct gttaccaaat acaacggact gtttcattgg acggttccct ccggcatgga
    64861 aatccgggct ggagcggaat gcgggcgcca catctgcatg acccattgtt attggagaac
    64921 aggcggagat gtttttggag gagcgcaaat catgggtgtg gtacacggat tggaaaaaga
    64981 tgccgttctg gagcacaagg cggcgattga ccgccatctt gaggcctccg gcattcccat
    65041 cctccacacg gctgttttct ggggtgaacg ttcggaaatc aagccttccg agatttctcc
    65101 ggaactgtat aggaaatgga tggaagaagt aaaaaaagct cccgttctgc agtaactccc
    65161 ttctccacgc ctgcctccgg cagtcttccc ctggggtaga acagcttttc cctccgaact
    65221 cccgcgaatg acgtcgcgaa ggcacaagca tttttaatgc cgcaactgag atgccggctg
    65281 tttcccctcc ctccgtcctg cagcaccgct tagttcccta ttcccggcct acagcggctt
    65341 aataccgttt tcccggacgt tccaggatct ttgatatatt ttcctgtcaa ttcaggaaac
    65401 gggactgaag aaatccgttt caagccttca gggagacgca cgcctcaacc tgatgagcta
    65461 ttcatgttct cctttgctcc ggcgctgttt cctttccggg atacaccggg agaggcttct
    65521 gcctgcagaa acaagaaaaa ctcaacacaa gggccgtctt cccccttacg gggagacggc
    65581 ccttattaaa gaaatatcgg ccggcgaaca acctgccgcg aagctttatt actgcttttt
    65641 ctgcggctgt gcgggagcct ggtcaggagc cttggcggga gcaacagcgg ccggatgctt
    65701 gtaggaaacc gcaaaaccgt catacttggc cggcagcttc ctgtccggga acatcagttc
    65761 atgcaaacgg tacagggcgg ccgcttcctt gacaggagcg ggacgggact tcagccgtga
    65821 atccggaatc acagcttcgg gattattctt cctggattcc acggcagatg agacgacggg
    65881 attctcaaag aatttgacaa catccggcat gcgcaggtag gcatccaggg agcgggccac
    65941 gcaggcagac gccagttctt catcggaacc atgcagcaaa accagggctt ccgcaaaata
    66001 atccagcgcc ttgttccaat cctgggccac catggcggcc gcacccaggg catatttcat
    66061 ccgcgcctgc tggaggcggg tggcattctt tttggttgcc aggaaagaac cggcacggct
    66121 tttcacgccg ttccagtcct tatctggaat caaggccatg atttttgcca cctccaggtc
    66181 attctgggcg gaaggggaca aattggcccc tctgacggga aacttggccg ccaacccttt
    66241 gactccagcc caatccatca tgcgcatggc gcactccagt tcatagacgg ccgcccgggc
    66301 ggacaggctg tccttgatgg aaagcgttga ggcatactta ttcaccagag cgtggaattt
    66361 gttgcgggct tcctggtact tccggttttc catagccacc ttggcatcca caaagtcgtc
    66421 cgtttcaaaa atatacagcg tcttgaaggc gctggccatc tgctgaagga cttcctggga
    66481 ttccctgctc tccacaaact ggaccgtggg tccattggag gaaagaatgt agatatccgt
    66541 cggacggcca ttcttgatgg tcggtgcggc aactctggcg cgcaggcgct ggggattctg
    66601 ggcattctgg gccatggacg gaaccaggga ggacagtccg gccaatacca ttattactga
    66661 aagtgctttc atatgattga ttactaaacc tcttgttat
//
LOCUS       DAWEMX010000002        63840 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_223_length_63840_cov_13.038159, whole genome shotgun sequence.
ACCESSION   DAWEMX010000002 DAWEMX010000000
VERSION     DAWEMX010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 63840)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 63840)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..63840
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223_length_63840_cov_13.038159"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3233..3242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    31564..31573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    47035..47134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    57301..57310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    57977..57986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    58348..58357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        15594 a        16676 c        17799 g        13621 t
ORIGIN      
        1 ttctctccac tctgaacgaa cggcgcaaat cattttacca ttcttgcaaa cagttccccg
       61 tgttcggcaa gggctggctg aaccgctgcg gcgatgcgtt cagcgtggcg caggagctcg
      121 tttaattttt acatcactcc ccctatgagc aagaaacccc gcagccaaaa ggccgccagg
      181 aaaaacaaga ctgccgacat tgagattttc gaggatcgtt cccctcagga aagaggatat
      241 cttggttttt acaccagcat cacgcccagg gtgctgaaaa atgcccgcga gagcatccag
      301 acgggcaata tgctcgactt ggaacgcgtg tttcgctcga tgaaaatcga atggccccgg
      361 ctgcggggaa acctgcggaa gctccgcgaa aaggttcagg cattggaaat taccgtgtcc
      421 ccctgggccg agaaaggcaa aaagccgaca ccaacggcca gccggcatgc ggatttggtg
      481 gaatccgccc tgtattgctg ccggattgaa cagggaaaat gggaactgga tctgaacgga
      541 ttgattgggg ccttggcgga agctccggaa cgcggagtgg gtgtgctgga aatcatgtgg
      601 aaccctggcc atatccgggc gcctcgcgcc tattgtccca ttccttccac attttacaaa
      661 tggtccagct acccggctca aatcgaccgt cttgtactat gtccggacgg agtagggtgc
      721 ggtcccgaaa tggaattccc tcccaacaaa ttcattgcct ccctcaactg tgacgggctt
      781 gaccatccta tctacggcgc caaccttctg gccctggtcg gttggttcgg cgcagccaaa
      841 ttcggcttgt cctggttcat ggaattctgc cagatatttg gatctcctct gcgtcatgga
      901 aaagcatcgg gaaccctggc gcagaagaag ctatttgacc agatggtgaa attcggacaa
      961 acgggcatcc ttgtaacgga cccggatgca gacgtgcaat ttcatgacgc cgtaaaagga
     1021 ggcaaccagc ttccgcacct gaacatgctc gaagaggcca acaaggcatg cgacattctg
     1081 attttgggcc aaaccctcac cagttccgtt tccagcacag gcggcaaccg tgcgttgggt
     1141 gaggtccatg aaaacacgga aaaccaggtt gtccttgccc gcgggaaata cgttgccggc
     1201 gtcctcaacc agcagcttgt cccggccatt ctggaactta acctgggaag atgcccggaa
     1261 catctgccct tcatctcttt taaggatccg tcctccggta tgagtgaagc aaaactgggg
     1321 tgggtggaaa gggcaaccag aattgttccc gtcgctgaag aacaggttta cgactggctt
     1381 gatatcccca tgcctgaaga aggggcgaaa ctctaccagc cgccatcttt tgggggattc
     1441 gggccggaaa cgggagaagt ggatgacctg gaccgggaat ccctggttca tgcggcgcgt
     1501 aaaaaaaaac gctgaagcac attgacgagg tcaaccggat tgcggcccgt atcgggcgcc
     1561 agacggacca ggcggcccat gaactgacct ccggtgttgc cggattcatg gaagacctga
     1621 ttgccgccgt agaagccggg gaagacctgg aaacagtcat ccggtccgcc cgtaggcagg
     1681 tgccgtccct gtgggacaaa atagatacct ccaagctgga agagcggctg gtcaaggtcc
     1741 agcatgccgc gctggaagcc ggctggaact ccatgcgcga atccaaaaag gccaacaggg
     1801 aaggatgacg ggccatgaac attgaaattg acatgagcgg ctttgatgcc gcgctgaacg
     1861 aagccgtgaa gattgtcgcc ccggaaacgc tggaagctgc gaaccgggaa agcggcgaat
     1921 acctgcggga ctatctggcg tcctggtacg acggcaaagg acgggaacac tggatcaaca
     1981 attctcttcc tacgcatggc ccgggacgca tggcgacagg ctggttttcc aatattgccc
     2041 gtaaatggtt cctttcctct gcggatgctt ccggagcggt tatctccaat cccgacgagg
     2101 acggatccct gcggcataaa ataaagggag ggacaattac ggccaaaaat tccggggcgt
     2161 tgaccattcc ccttgttccg gaggcccacg ggcgccgggc tgccgattac cagtccgaaa
     2221 tcggggaatt gttcaccatc cccaacaaaa acgccctgtt tgaagccgtg gacggcgggg
     2281 gagtgcgtgc ggtgtatgcc ctgcgccaat ccatcacaca ggacccctgg ccggatgcca
     2341 tcccgaccgg cgaagaactg atcagcgcct atggcgtcaa gctcatggac gttctggcgg
     2401 cgtctcttga tgcctgaaac acatatccag tccccactat ttacgctttt ccgtttcatg
     2461 ccatgcttgg ggcatggatt ttgaattcaa cgttcctctt gcgtttggcg acgctccggc
     2521 ctgtatcgtg tacatgccgg agggggaaca tttcatcaat gcatccattg gaggacggca
     2581 gaaagtgatt gtggaccgct cctgcctgga ggctttgcag cgggaccttg cgttgaagct
     2641 cactcaaaac gtgcggcccg tctgctactt tgaccacaag acgggacccg cctcctttat
     2701 tcccgcttcc tttgactaca tggacggcgt gggggtcatc ctcaaggggg aatggacgga
     2761 aagcggcagg aaagcggtgc tggggcgtga ctacagctat ttttctccgg cattcaagct
     2821 caacacggca acctgccgcc ccataggcct tgaaccggat gacattgagg ttggctctct
     2881 ggtgaatgac ccggcctttg agaatattgc ccgcattgcg gccggcaagg ccagacttga
     2941 gaatttcacg gttcttgaac cggacatgct tttgaatagc ggcggagagg ataccggtgc
     3001 tgttcatgac caaacaaata acacaaatac aacaatgtac gaactactgg ttaaatgcgg
     3061 cgtcctcacc aaagaggaag ccgcatctga taaggccggc aagatcgcag aggacaaaat
     3121 caacaacctg aagaagaaat ctgagggagt cgagcagtcc aaaacggaac ttgaagcggc
     3181 ccgaaaagag gcggaagacg ccaaaaaaga agcagccacc tgcaaggcgg ccnnnnnnnn
     3241 nnaagccaaa ctgaaagcgg cggaagccga gcttgccgag gtgaaagcct ccaaggcggc
     3301 tcttatcgac gcggaaattg aagccgccat caaggccggc aagattgctc cggaagatga
     3361 agatgccaaa gaggccttga agaccgctct aacggccaat atcaaggccg gcaaagctct
     3421 gatcgcctcc ataaatccga atcctgcttt tacgacggtg gtcgccggaa aggccaataa
     3481 cggcaacggc gggaatgagc ctaccggacg tgaccgcatc attgaaaaca tcaacaagga
     3541 aaagaactaa gccatgtcat ttttgactct actggacatt cagaaacgca atggttcgga
     3601 atccgacatc ggattgatcg aagaagtggg gcgctccgcc ccggaagtaa cgcagcttgc
     3661 ttccgtcgtg ggctccaaaa ccatcgtcaa aacctatgta cgcaccggca ttccccgagc
     3721 ccggttccgc ccggccaatg cacccatcgg ttacacgtcc tgcacttacg aatcaaggaa
     3781 cgtggaactg tttcccattt cctccatcgt ttttgtggat catattacgt tggaaagctc
     3841 tgacgacggg gaagccgccg tcctggccga tgaagcttcc gggattacgg agggggttct
     3901 tctttccctg ggagcccagg gtttttacgg aaagaaaatc gacaagaacg gttttcccgg
     3961 acttcccgat ttcatcgacg atacgatgat catcagcgca gacagctcca aggccgccga
     4021 caattacgac gggacgtccg tatttgccgt tgtggagggt cccaaaggcg tgcattggcg
     4081 ctgggggcgc gacaagggaa ttactcttgg cacgttcaag gatgcgctta ttcccggcaa
     4141 ggatccggaa acgggcgagc cgggtgccat tcccggcaag gttgccgatt tgaccgcctt
     4201 tgtcgccctg gtcaacaact ccaagctgtc cgccgcacgc ctgaagaata tcggcacggc
     4261 agaaggaacg acgctggatg acgataaatt ggcggaactg ctggctttgt tcccggcagg
     4321 cgtccgcgtg acgaagttca tcatgaaccg catggccttg gagcagctcc gcaagagccg
     4381 cagggtagcg agcgtttccg tggacggagg caaggcggga ggggattcct ccggatccgc
     4441 cccgattccg acccacgccc acggcatccc gattttggtg acggactcca tcgtcaacaa
     4501 cgaaagcgac ctgtcctcca tcacgggcat ttcctactgg ggcaagcatg cgccgaagaa
     4561 agtgagcaac aaaaagaacc aataagacgg aaaggaacct tagacagtga atcctatcag
     4621 acacacccgc aaggacgaat tgctgacggc ccggatgaac atgccgggca cgggcaagac
     4681 ggcctattcc gaagtgctgg atgcgggtca gacgggcggc attgatgaaa tgtccatcgt
     4741 cattgagcac gaagaccttc cggctctggc tgccggaaag aagatcacgc tgaccctgga
     4801 agcctccggg gatggcgata gctgggccga ggtgccgggg ttctccctgg cacctgcggc
     4861 gggagaagaa gcgggagctc ttgcgaacag cattgccgga cgggctccct atggcatggg
     4921 acgctacatc cggctcaagg ctgtggcaga caccgccagc ggcgacaata ccgccgccaa
     4981 atgtgaactc tccatccgcg tgtaaccgtc atggcgctgg tccagatcac ggaaaacacc
     5041 ctgcgggcct tcctggcgga cgctgaaatc gcggcgtttg actcggcagg agcggaaggg
     5101 gattacccgg aacgggccgg cgccctgatc aggacgacgt gcaacctcgt cgccggaatc
     5161 gttaattctt ccgggaaata tcccattctg gcaacaggtc aggacagggt gccggaagaa
     5221 ctggaacacc cgacgcttgt ctggatccgt cacgccatgc tggccgactt gcccgacatg
     5281 ggcgacctgg aaggatcccc ccgcgccaag cagtacagca cggcaagcga gattttccgg
     5341 gccgtccggg agggcaggtt ttatcttgcc ccctacgatt cggagagtgg cggtgtagag
     5401 gtgtacggag ccgggcagcc ctatcagaac tggtgtgaac tatgagtgct ttacccgcaa
     5461 gcccgcgcat cgccgccggg gaaaaaatct atcggaaaat cattaccctg tgtgcccgtt
     5521 acaacagcgg ggaggatccc ggcattgtca tgcgcggatg ggatgccgac ctgaaaaccc
     5581 tgattactca aaagctgtcc aggctgggaa tctgcgtgct ggtatgtgcc ccgaaacgaa
     5641 aacccctgca ggaacaggga ggcacgaatg ccgtcattct gaccacaaaa atcgtcattg
     5701 aaagcaatcc cctcctgaaa aaatccgacg caaccgccgt cctcggctgg gatgcggacg
     5761 atctggcaga cctgctcgcc atcgggcttg acgggcatcg ggaaccgggc tggctcacct
     5821 gcatgaagct caaagtcaca ggcacggagt caagccgggt acagatggcc aacaaggctg
     5881 ttacgctcac ccttgaacaa accaccatat tgaaacatgg caactaaacc caccaccgcc
     5941 gcggcccagg aggccgctac tgctccggcg ccccgcatcg tcaaatgccg ggtggccgtc
     6001 aacaagctgg aactccctca cggcatcgcc gcgcggggaa aaatcgtcca catcccggaa
     6061 gacgtgtaca aagtccacgc cgacgccggg aaagtgacct ttattgacta cgtaagaagc
     6121 taacaaccat gtcagaactc tacaacaagg aaatgctggt cggcaccttt ctcgacctgt
     6181 gcccgttcgg aacgacagtc acggccgaaa gcggcacgga cacggtggac gagcatttca
     6241 agccggcgaa ggactccgac gcctggatga ttgccaacga agtcatcgac tacaaaatca
     6301 cgccgaccac ggaagacgac gcccgcacgg tattttcccg cgacacgacc tcctatgtga
     6361 cgcggaagaa caccaaagtg acgggcaaca ccatcgagat taattccacg gaggttaatc
     6421 cggtctgctg gcaggtgatt taccagtgcg acaggctgga agccgggaag gaagtgcagc
     6481 ccttttcccg gaacatctac gggcaaaagg tatgggcgcg cctcaccaaa taccaggaag
     6541 acaaaaaaga aatgatggtc ctggaagtcg cggcgctgct caaggtggaa atccccacgg
     6601 aaaacaacaa gctgatcacg ccgaaattga cgcttgaagt gataccgtcc tccctgaatt
     6661 ccctgacgcc cacggaagaa atcgccttcc cggcctccgc cggagcatga cagccggggc
     6721 cgcccctctg tttgcatggg gaggggcggc ccctgtttcc cccaccatta ttgaggcatg
     6781 gacacgaccg tctctccctt ttccattacc tttgacgggc gccccgtcgt gcgcgccggg
     6841 gaattcctgc tcgactccct gccggaacac gctttcccgg tgcagttcgg cacgtccgcc
     6901 acgccgatca tcaacagccc gttccccagg ctggacgcat tcggcaacct gtccctgtcc
     6961 ttcaccatct ccaccgtgcg ggaatgcgcc tcccacatgg acgcgtggag cgccttttac
     7021 gaatggctca acgaatggaa aacggcgggg aagggggaat gggcctggac cgacgcctgc
     7081 ggccgtgaac agcgctttga agccatcatc gccgacgccg agccgaaggt tcagggcctg
     7141 cgccttattg tctcctacaa cttcaccctg ggccgccccc tatgaaaacc cttgacgtat
     7201 cttccgccga cttcctggac atggccgaaa gcccgtccta caaccggctg tccttcgggg
     7261 gagcctccgt ctccttccgc gtgcctgtct cccggtttgc ctcctgcccg tttgaagaag
     7321 gggaacccgt caaaatcgtc tggcgcggga aaaccctgct catcggcccg gtcatcgacc
     7381 tggaacattc ccttgaagga acctccgaga gctgggacat caggatttac gattactggt
     7441 ggaacctgag caacatccag tacttcgcga atggtcgcgc caacggcatc tttgccgaat
     7501 accgccgagg cacaggcgga agcggtcagg aaaaacaggc gaccgcgaac atccgggacg
     7561 ccctctccgg agttctggac cacgccgtca gcacggctct ggttcccatc aaatacgacc
     7621 tccggataga caaggatgcc gaaatcattc cgtttgccta ctcgtcggaa acgtatgcct
     7681 ccctgctttc ccagatccag aaatggcgcc ccaatatggc cgcgtggttt gaatacggcg
     7741 cggacgactc cgccacgctg gtcattgccg accatgccgc cctgccggat gtcgtgctcg
     7801 acctgtccgc cgtggacgta agcgccctgt ccctcaaggc gcgtcccgat ctggtgcctc
     7861 cggccgtggg gctgacctgc aacgcctccg ttgtctcccg gattcagcgc gcgctggccg
     7921 tctatccctc aggcgcctcc ctgtcccagc cctatgtggt gacggcggaa gtggacgttc
     7981 cgggcggcgt caaggtctcc gacactgccg ggcaatacag ccctgcggaa acggactcgc
     8041 tgggttacga cgccccgcgg atgattgtcc ggggagacaa attcccgacc ggcacggccc
     8101 agtgggcggc ccgcgtcaaa cgctgggctc cggccctgga ggattgcgcc ggcctggaag
     8161 tggcggccag tccgaaaatt acgtccatca cgccggctga cgcggaacac cggggataca
     8221 gcaatgcggc catcacccac gaactgacct ccggccagat aaacggcagg agcgcaagaa
     8281 tcaaatgggg caaggtccgg gtggatttgc gggtgcgggc gacggaaccc cccgacacgg
     8341 tgaagcaata ttttccggaa tacggcggaa aatccggaac cggggaccgc tggatcggaa
     8401 cattgacgtt tgaagtgacc acgacgaatg tcggctacgc gtcctaccgg gtggacaggg
     8461 cagggacggt ggaaagcgta tccgacgacg gcggaagccc cggaggcgac gaaacatcgg
     8521 gcagctacga cacctccgtg ttgtataaaa atttcctgaa atcctactac gaagccaccc
     8581 gcgcgctgcc ctatgacgga tccgcgaccg tccacgacga ctttgaccag gtctgcgggg
     8641 ggcgcctctc catcatggga ggattgaaag aatgggaaac catgcggtcc gtcattcagg
     8701 aaatatccct cgaccttaaa acgggagttt ccgacgtgac ggtgggggcc ccggaacaga
     8761 tctctctgca ggactccatc gaccggagcc ggcagcttgc cgaggcgctg cgccggacgg
     8821 cctgggcgga ctcgtccacg tcttccgggg gaggttcttc gggcggaggc tcttcgggcg
     8881 gaggctcttc cggagcggac gatgaggtcc cggagattcc cagcgtcggg ccgtccgtaa
     8941 aactgctgca ggctcaggag cctcccgcgt ggggaacaag cgccgttgag gtgggattcc
     9001 aatgccgcct gtcttacggg agcgacggca aggtgtccga tgcctacatc cgccagggga
     9061 aggccgttta tgccggcagc tacatcgggg ggctgcttcc ggaaggggac ggctccgggg
     9121 gatgggtgaa aagccccgtc acgtccgggg aaatctggct caagatccag ttggacaagg
     9181 acacgaaata cctcggatcc tctctgtccg ccgcgggcgg cgtctccgac cccgtcaggc
     9241 tcgcggagga agaccggaaa accccttatg aatattattt ccatctggcc accatcgacg
     9301 gcaacaaggt ggtgcagcac caggcgggca cggtttatct ccaaatccac ccgggaacct
     9361 tcggcccctc cggaatgtca tgatcaggat atacaccttc acctatgccg gagacgcgca
     9421 ggaagccgtg gcctgcgtcc ggtgcgccag gacggctctt ccggaggcgg tagttacggt
     9481 ggtggacgac agcgccgccc cggtgccccc ggaggccagg agggcccttg tagcgcatgg
     9541 ggcgcggtat cgccggagtt ctttcccccg ctgcggcaac ctgcgcggcc cggagtgcgt
     9601 ccggggaatt attgccacgc tggccggggc ggcggcggat ggcgacaccg tcgtcaagat
     9661 tgactccgac acggctcttc tgtcgggcgg ctgggtcagg gaaatgaaac acaacgggct
     9721 tgcgctgcac gccgccggat accgggtccc ccggaacccg tccgaacggt ccgcctacgg
     9781 aaattgctac gccctgagcg gacgggcggc caggatggcc gccgaagcgc tggaatgcgc
     9841 cgccatcccc ccgctcgccc cggaagacct caccatctgc cgggccgtca tggatgtctg
     9901 cggccgggag cgtgtccggc ttgacgagcc gtggacgccc ctgaaccggg ccgggcggtg
     9961 gtcctggtgg aactgggaca gccggacggc gaacccggag gattatgccc gcagctatga
    10021 cgtggtgagc gtcggcaatc cccggcctcc ccacgtcccc aaaaacgccc gccgggaagt
    10081 catgcgcgcc ctgtgcgacg cccgtttgaa tctcaacagc caatcaatag aaccatgtca
    10141 gacagaaacc tgaacatcaa tatcagaacg accgccgaca cctccggcgc cactcaagcc
    10201 gccgcgtccc tggacaggat acgggaatcc ggcgaatcca tgtcgcagac ttccggcgtg
    10261 atggaccgga tcgcagattc tctttcccgt gtcaaaacgg ctgccgggga aaccggcgac
    10321 gccatgaagg acggcatggg cgcggaatat gaaaaagccc tggaaaacgc caattccaaa
    10381 cttgaccaat acgccgacgc cctgaccgcc gccggctccc ggatgaaagc cgccttcaac
    10441 gacaacccgg gattgaccgg gtttattgac gaagtcacca acgccgtgct gacctccgag
    10501 gaattcagga agaagctgga acaggtggat gacgtctttg aagtcctcaa caacaaaatg
    10561 tctgatttgg atctcggggc gaaatgggga gatgaccttg acgaaaacct tcaacaaatc
    10621 atcgacggct acaacaagga aatggacgcg gctgacaagg ccgcggaaaa ggcggaagcc
    10681 gcggaggccc ggaagcagca ggccgctgca gccacggtag aacggctgga agccaacaac
    10741 cgccgcgcct ctgccaccta tgaggaacta caggccgaac tggaatccta cattgccaag
    10801 ttggaagaag cccggaaggc cggggacaac gtggcccagg cggacgccct gaagaatatc
    10861 caggacctgg gacggcgcat caagacggcc ggagatgccg gacaactcac ctccacgcag
    10921 gtcaaggggc tggcggggca gattaccata gcggcaacgc gcattctggg catgtccagc
    10981 tcgctccggg gagccatccc attcattcat ttattcggaa ccaccatcaa aacggcgatg
    11041 gggccgctgg gctgggccat gctgctgatc caggggctga ccgccggcat taccgctttg
    11101 attgaccact tcaagaccaa aagcgacgaa cttgacaagg ccgccgagaa ggcaaggagg
    11161 aagcatgatg aaatcaacca gtatttgagg gatgctgaaa aaggtcgtct tgcgcttgtc
    11221 cagaaaacga aacaggaaga agcggcccac gtcatcaacc gggagtatga acaacatatt
    11281 aaaaacatta cttatgaata tgagaaacag acccgggaaa ttgaatatca gctcaccctc
    11341 cgcaaaatgg agattgctga gaagcagggg gcggacacct ggaaaaacaa gatgcagcgt
    11401 ttggaagtgg aagaacagtt ccaggacggg aaaatcggca agcatgaaag aaagtacagg
    11461 cttttgcttc tggatcagga gcttgaaagg ataacggagc gggtcaggat tcaaactgcc
    11521 catgaagaag accttgcctt ggctgacaaa cagggggatg cggaagggaa aagaaataca
    11581 gctttaggtg aagcttcacg cctgcaaaat gtaaaaatgt ctcttccaac tgtagaggaa
    11641 atgtcaaaat tatttaggtt agaaagtagt gaattgcctt atataattaa agagataaaa
    11701 ggtaaggaag aagctataaa atcttttaca gatttgggtg gttacgaaga ctttgttaaa
    11761 caacttcgtg gcgagctttc agaacttgaa atacaaaaag ataatatttc gggaatgatt
    11821 gaaaaaataa aatctatttt taatgaagaa agtatttctt ttcaaccgtc ttatggattg
    11881 gataaaagtg ggaagcctat tactgatgaa caaagaaccg aagaatataa aatatccctt
    11941 gaaaagctcg gagcccggca acaaacagcc gtagaagagg ccggaaaaca tcaggaagaa
    12001 ttaaatggta ttttgaaaga acgtgttatt gttcaaaaag gaattttacg acttgaaacg
    12061 gaagaagcga gaaatgaaca agctcgaaag aaagaatcca gagttgggga aaaggaatgg
    12121 aaaaatgatg ataaaaaaga agagcaaaaa gcagaaaaga aactcgaacg ggagagaaaa
    12181 caggagctta aaaggcttca acaggaacag aagcgggaaa ctgataaggc tattaaaact
    12241 tttgtggaag gctttaccat caacgccgga aagagtgcta ctccacatca aacctctcta
    12301 gtccacaagg ctattgacgg cattagaaaa gatatagaac gcgccgcggt cgatggatac
    12361 attgacccgg aagaactaca gaatttaaac aagctttttg tgggaaaatt aacggagctt
    12421 ggagttgcat caagacaagt tcttcagggc ttgaaacaga tgtttgaaag caatattgga
    12481 gctatcaaca aggaaatagc agaaataaaa aggacgcaaa gaatgcagct tagccgggaa
    12541 aggttgagaa gatgataggt aacgtcttag ttccctactt ctagtcataa acaaagaaat
    12601 cgacgtgcag aatttgcgat tagaaaactt tgtgattttt ttctttttat tatcattgtt
    12661 gcgattatca tacagttatg aagtattacc ttgctacacc ggaaggaaaa acagaaggtc
    12721 cttttcatcc agagacgtta gaggttttgg ctcgacgtaa aagtatcact tttgagaccc
    12781 ttatatgcag ggagggggga aatgaatgga taaagtatgg ggagtttctt tcctccgcaa
    12841 ctcacgaaaa gtcaaattcc tctaaaatga atatttgtgc tgaatcagaa agaacccgga
    12901 gaaataacta ttcagcaagc ggagtatcag aagcgttccg gtgggtgggt atcgtttgct
    12961 ttgtactggc tataattatt ccccttatac ttttgtcctc agggagcgac cagagcattt
    13021 tcgccgcttc catttttctt gtgcttgccc tatccgggct gggatggatt ggagttgctc
    13081 atattatttc cctcttagaa cagattgctg agaacacggg aaagtgagta ttcgtgaaat
    13141 caaaaatagt taccaagtag ttacctatga atcgtttttt aataaaaata ttgaaatatc
    13201 aatgttttga atttgtctta aaatctgttg tccgcaagga cgtaccggtt cgagtccggt
    13261 cgggggtact ttatcgaaat taaatcttgt aaataaaaag cccgctgttt gcgggccttt
    13321 ttgttttgct tcaatatcat ttgctggctg attctccatc aataattttt gttttcccgg
    13381 atgagtaaaa caaggatggt ggtgtttcgt ggctattctt gtcatgaaat gcagaaagca
    13441 gagagaagaa tgttgagtgg aaacagttct gaaaaaagag tttgtaacag ggcgcctcag
    13501 gctgaaaaat agaaatacgg agtatgaaat tgttgggtat cttccggttt ggaaaatcag
    13561 atggttcatg gcgccctgga aaaaagggaa tgctgctggc gttgaaaata gcaggaggca
    13621 tgtttgcggg agcggttgtg gtatgcgttg ccgcagaatg gtacatttat tccgtttcag
    13681 agggcaggat tgttgagcat tccagggatg ttcccgttcg tgctcctgtt ctggtgttag
    13741 gatgctcccc aacattcatg gggagcccta acggatattt ccataacagg atggatacgg
    13801 cctcggaact ttggaaagat ggaaaagcaa cggttttcat cgtttccgga gataatagtt
    13861 ctcatgctta caatgaaccg gaatggatga aacaggctct ggtggaacgt ggcgttccag
    13921 aggaacggat tgtatgtgac tttgcgggat tgcggacttt ggattctgtc gtgaggatga
    13981 aggaaatttt cggcgtttcg acgatgattg ttgtttctca ggaatttcat aatgaacgcg
    14041 ccctggcaat cgcagcgcat gaggggatgg cggcctgggc cgtcagcgcg ccggatgttc
    14101 ccaaccgccg ttcccgtatg aaaagctggt tcagggagcg tgcggcccgc gtatggatga
    14161 tgctggactt gtgggtttgg agccgtgaac cccgtttttt gggagaacct gtggttttgc
    14221 cggaaataaa agaggaaaaa tgaacaatac ttcatgcggc agggctcgtc ataagatgtc
    14281 tttgatgcgg tacggcaacc cagaaggagg gtgtgcccgc gcctcgaatc attaaatgct
    14341 gaatggtcca atcatgtcgg ctacgtggga tgatttgcca tgaagatgaa caaggcctga
    14401 aagcctgagt gaacaattcg tcatgaagac ggtcggaggt gatggccgta cgtttcattg
    14461 tccttcatgg aagaaacagg tgcaatcctt ccccttatct tatgcgtatg ggtgggcaac
    14521 ggttgaaaaa aggatggttg ggaaacatca gaaagctgat gctctggtcg gaataagttt
    14581 gcctccatat ctttagaaaa aacactccgg aacaggttct gttgcgtttt gatgcggctg
    14641 acgctccctg ttggaattcc atatttgatt aaaaagggga aaggatacgg taaaaatcat
    14701 tgactctttc cgggaaagtg ctatgcagat ttcagcggct tgtgaaaagc gggctgtttt
    14761 attccggctg tcatgttccc ttcttctagc ggtcaggaag gccgtctctc caacggcaaa
    14821 ggctcggttc aactccggca gggaatgcca tccggcagcc atgttttgtt tcctgccgtg
    14881 aaaaggcggt aaattgcatc aggaaaagca aactacgggg gagttatgat ggaaatacgc
    14941 tttctatgac tttccccgtc cattccggta gtggctttcc tccgaataga tgaagaagca
    15001 tgggagcggt attggtggta ttgtttggtt cggaaatcag gaaatttctc ttaatgcccg
    15061 gacctgaaat tccccatgga actatcattt ccgctgcgct cgtccccccg tggccctttc
    15121 cgattccccc atgatccgtc agaaatagaa aatgcgtatc gttgaataag tcttcctgcc
    15181 tcaatttctg aaaaaagatt ccaatttgct gatccgcctc ttcaatagat tgaaggtatt
    15241 ctctggacat ccagccgtat ttgtgccccg catgatctgt atgaacggaa tagagaaaaa
    15301 ccacagtcgg gttgtagcgg tgtttcttga cgaattggaa agcctgatgg taattggaag
    15361 cataggcgtc gtttgccagg tacttgattt catccaggta ttttttgttg aacggatagg
    15421 caagaggttt ccaattatag taaaaagctg ttttgcagga aggctggcat tttttcaggg
    15481 cctggaatac ggagggataa tatccgtctt cgtctctccg tacggcgggg taggaggaat
    15541 ttgccggcgt ccagttatta ttgaagactc cgtgctgttc cgggccgctg ccggttaagt
    15601 gactggtcca gttaggtaac gtgacggagg gcatcacaac ccgcgtatcc agagaaaaag
    15661 ctccctgtga catcattcct tttaaagagg gcgcatgcgc cttttgaaaa ccttccacac
    15721 aaataccgtc cagggaaatc atgatcactc ttttggcgga attatcggaa gttctatctg
    15781 gtacggaatt tctgggaatg ccggaatcat attgtgtagc ggcaagggct gtattgcccg
    15841 ttgtcattcc tgctgccgcc aggcctaatt gattcaggaa attccggcgt gttgtttgat
    15901 gataagagtt ttccatataa agtacatact gttggcttga agtattttta tcaacgcttc
    15961 tggatattcg ggaagctcat ttcatcgtat agttttttac ggacaggggc cgaatccggg
    16021 tttcaaagca atttttccaa taatttgttc ctttcctgtg atagaatggt tttatagaga
    16081 aaggatatca tcatttattg tattcaaata gttaagtggg tgtgacaata taggaatgct
    16141 ccgcaggctg tctcatctgc gatttaggca aagcatcttc catacgcttc aaacgagggg
    16201 gcatatcata tttgttacgg gccgtatcct catcaatgtt ggccaaaatg ccgggcggga
    16261 gcggagactt tcaatctgga cgttttacac aggatgacat tttccgcgag ctggagcaga
    16321 tttttcaagg aagtgggtgt tacgcgctgc cgtgtggtac agtcagctcc ctgaagaaag
    16381 atcagccgga ggcagacaga tatccctgat gttattcctc caacaagacg ggacaagccc
    16441 ggcaggtgtg ggtgtttgcc agccatcacg atgctcgcgc cgccattatt aaatcagcca
    16501 attagcataa tgataccatg agagagatac aaatacagtt ttccaagccc ggtaactgga
    16561 gggaattcac actaacagcc atctaccagg attcggatgg atatacccgc atagaccgct
    16621 ataaacagaa tgatataccg tccgggcagg ccccggcttt gagcgcggct gtagccgtca
    16681 ttgcagacat ggaagaggac tggcaagccg tccaggtatg ggcaaggctg ggaaatacct
    16741 ctgttctcaa taattccgca ggagacgatg aggccgttga gtttcgtgaa gcagttctac
    16801 tgacgattga ggctgtcaat agcctcgggg gccgccgtat ttttgccccc ggtaattatg
    16861 cgcagttcat actgatggat tttgcctcca tatcgttttt caagtatttt accatcagaa
    16921 aataataagg atatcagcaa ataagattgt tggactgact gcatgattgc ggcagactgg
    16981 gggatatgtg atgcagtgtt ttttcccgga caaggcgttt ccggatttgt cgatgcattg
    17041 caacagtttt aaatggaaaa gatgttcctc ccaggcagga gcggatcaaa aacagggaat
    17101 caatgacagt aaccccatga gtgacgtaaa gcagttcctg gtcaacgttg ggaacaggaa
    17161 gggcctggtc tgtcgtgatg cctcaaaaat tgcagattcc cttatgctct gatgtttatc
    17221 ggaaaacctg gatggagtat cgtcatgcga ctttaaaaat acagtacatt gtttctaaaa
    17281 gccgttatca tgtactgcgg ttctataaaa acaacaaccg caagcattct atttcaaacg
    17341 cttgcggttg ttttgaaatg gtggaggcga cgggagtcga acccgtgtcc tgaaggccat
    17401 tgatgacggc atctccatgt tcagacgcat attgctgctt tcgcggctgc ttcgctatgc
    17461 gtcaactagg caagctcgct agttgcctgt ggaagtctcg tgcgttgcgc ggcaaccccg
    17521 cattgcacca gcctgctgtg tcttcgtcgt cccctctagc aggcgttgag gctctgacgc
    17581 ggctgaacta attaggcagc cagagcaagg tcgttagact ctgcatttat ttttttaatc
    17641 ggctttttag aaggccagcc gattaacctt cacatgcagc cagcaccgca gacttccagt
    17701 cgaaaccatg gcgcccccat atgtatttac cagtagagac aaaaaatccc gccgtgatgt
    17761 tcaggcagga atcattttat ttgctggcag tgatgcaagt ggcacgcccg tagggattcg
    17821 aaccccaaac cttctgatcc gtagtcagac gctctatcca attgagctac gggcgctttg
    17881 acttgcgtcg tgtaccgcgt aagcggtgaa ggaaatatag atttttcttc gggaaagtca
    17941 agatgtttgt aagatgattt gagaaaaaaa ttcatttttc tctttttggg agaataaatt
    18001 ttcagaaaaa gaaccgttaa ttgagggggg gagaaaaagg ggaaaggcgt tgcgccatgg
    18061 aatggcctgt ggcacactaa acggagaaaa tgagcgagtt caaggtgatc agatcgccga
    18121 tggaggcatt gaagaagtac ttcggctttt ccggcttgag ggagggccag gatcaagtgg
    18181 tggcttccat tatggaaggc cgcaatgtat tggtcgtgat gcccacggga ggaggaaaat
    18241 ccctgtgtta ccagcttccc tccttgtgcc gggctggggt ttgcctggtt gtcagtcctc
    18301 taatcgcttt aatgaaagat caggtggatg ctcttgtcgc cagagggatt cccgccacca
    18361 tgattaacag ctccctgagt tttcctgagc aaaaggaacg tttggaagga atgaagaagg
    18421 gtgcgttcaa gctggtatat gtggctcctg aacgttttgg tcatgaaggg ttcatgcatg
    18481 ctctggcgga ggtggatgta aatatggtgg ctgtggatga agcgcattgc ctgagccagt
    18541 gggggcacga tttccgtccg gattatctga agctgggaaa agctgttgaa atgatggggc
    18601 ggcctcaggt ggctgctctg accgccacag ctactccgcg ggtgcgggaa gatattctga
    18661 aacatttggg gctggatgat ccggtgacga tcgtacgagg gtttgcgcgg gaaaacctgc
    18721 atttccgcat tactgcgtgc gatacgcaca aagacaaata caagaggctg tacgaattgg
    18781 tgaagcgtta caagacgggt attatttatt gctccacccg caaaaaagtg gagcaggtat
    18841 atgaggctct ttccgatttt ggattgagcg tgacagccta tcatgcaggc atgacggatg
    18901 aacagagaga ggaagcccag aacgctttca tgaaccgcca tgcggatatt gttattgcca
    18961 cgaatgcttt tggaatgggc attgaccgtg cggacgtcag gtttgttgcc cattttgaga
    19021 ttcccggcag tgtggaggcc tattatcagg aggccggccg agccggacga gacggggagg
    19081 aggcttattg cgaacttttg ttcaaccatg cggatttgcg gacccaggaa ttttttattg
    19141 aaggagtgaa tccgggaatt ccattgattg tggagctgta cgagttgctc aggaagcatt
    19201 gcagcgcgga aacccacgat gtcatgtggt ctctggagga aatggcggag cgtctcaaat
    19261 gccgcaatgc catgcaggtg ggagctgctt tatccgttct tatacgcaat ggagcgatca
    19321 gccgtcatga cgttccggga caacgcgtga agcttaccag agtgacggat ccttccgtaa
    19381 gcggcttgaa gattcctctg gatgaagatg ccctgcggga aaaggaagtg agggaccggg
    19441 aaaaactgaa ggcaatgacg gagttcgctt attcctccgg ctgccgtcag caatggattt
    19501 tgaattattt cggggaggaa gatggagtgc cgtgcggccg ctgcgaccag tgcttggccc
    19561 tgggcgttga ggaaggccaa tccctgggag aagaagagac tttggtggtg cggcaggcgt
    19621 tgagcgggat tgcgcgcgca tccgtgcgtt tggctgatgg ttcctggcag ggaaggtggg
    19681 gcaggacgaa aatcatccag atgctcaaag gggcgaaaac ccaggagttg ttaagaacat
    19741 ccctggtccg gctgagtact tatggcatcc tttcccgatg gagtgaggac gatatccgcc
    19801 agttgttccg tgccatgcag atggcgggat tgaccagaat gagcggggag gcggaccgtc
    19861 ccctgattac cctgagtccg aaaggaaatg aagtgatgat ggggcgcaag aaggcatctt
    19921 tggtttggcc ttttgcccgc cggggaaaaa tatccgtatc tatggagcag gccagagttc
    19981 ggtctacggg aaatttggct gccctgggag agtttgacga ggatttgttt ttgaagttga
    20041 aggagctgag gaatgaactg gcacgggaag cggggattcc ggcttatgcc gtgtttcata
    20101 attctacact ggaaggtttg gccaggttga aacctaccac ccgccgtggt gctatgaacg
    20161 ttcacggcat tggagagcag aaagctgctc gttacttgga tgatttcctg gaagtcattg
    20221 ccgagcattg cggggtgtga atttgtcttt ccgatttgct ccaatcaggc gtacgccgtc
    20281 tctggaacgt ggtgttcatg ctgaactgag gaactatttt tcatgaatta cggaaaaaat
    20341 gttccatgct ttgcgaatat atttgccggg aaaatgcgta tttgtttgaa taaattgttc
    20401 atcaacatca agttgtggat attttgactc tttccagaag gttgttttcc tgccctcatg
    20461 gaacaatcaa atgaatggct gtctatcccg atccagtagg gtcgagaaga ttgcctgatc
    20521 aatatttttg actgtattat ttatgtattg acttcatttt gggaagcgtg tagtctcctt
    20581 tccacacatt acccaagacg gagcggtggc tgagaggctg aaagcactcc tttgctaaag
    20641 gaacgtactg gcaaaaccgg taccgagggt tcgaatccct cccgctccgc cagttttctt
    20701 ttattgtggt ttatgtaaag cctgatttta tcaggctttt tttattttcg ggcattccag
    20761 tttattgtgg tttatgtaaa gcttcctgta catggagctg tacatgatat aaaaaatgta
    20821 cagcatggca ggaatcatca agagaaataa caaatgggtt gccgtcttcc gaaccctgga
    20881 cggcaaagag atcagaaaaa caacccgcgt tgacgttgtt ccaaaggtta ttcctcccgg
    20941 cgtcaataaa agagcgttgc tgtctcaaaa tgaagccagg gctcgcctta ttgctgaaga
    21001 actggaaaac ggatataaga cgggcttcgt agactcgaat cgtcttaaag cgatagcggg
    21061 gggagatatc cagtcattca gccttggaag aaaggtggtg agtgttggcg cctatttgaa
    21121 ggaatggctg caatcaagat cgggtaagat tcaggcctat gaacgggacc gaaaggcaat
    21181 ccagcagttt atttcctttt taggaaatga tgggggacaa tcaatctcgt ctgtgaacaa
    21241 aaacgttgtc agggagtttt ccgaggtaga aatggagcgt gtttcatcgg gtacagtatc
    21301 cagatacctt gaatctctat catgtgcttt taatcaggcc gttgaaaaag aattgattag
    21361 ctcaaatcct tttagagggg caaggctgga caagaaaaag aaacaggctg ataagcagga
    21421 acggggggct ttcactgtag aagaagtcaa aaggcttgta gagattttgc cggacgaatg
    21481 gccggacatg ataagggttt gtctttacac cggaggccag aggctgggag atattgcaac
    21541 gcttaaatgg gatcaagtaa atctggaggg cggcctgatc tccatgacca cccagaaaac
    21601 caagcggcgc atgaacaagc ccatcattgg acctctgaaa gagattttga aagaaaggga
    21661 aaagtattct atcaatgaat tcgtattccc attggcggcc atgaaacatg ctcaagcggg
    21721 cgggaagtcc agcaagttgt ctctcgaatt tacagggctt ttgaaaaaac atggcatcat
    21781 ttcctcggat ggaatgaaag cggaaggtga taagcgtgtt ctatccgaga aaagctttca
    21841 cagtttgcgg gctacagcag taacgatttt aagattggct ggagtacctg cggatttgtg
    21901 ccgcttcatc gtgggtcatg attccgaaga gattgagagg gtttatttcc gccctgattc
    21961 acaggatgtg gttcacgcca tggaagaaat ctcaagcaag attattttgt aatcccgtcg
    22021 aaaaaaaacc gcccgcgtat ccctacgcag gcggaaacga taaatcaata aggagtgttc
    22081 ctcaggaaca gctcttttat attatatttt cagccgtcgt caatcttgga acctcgaatg
    22141 agggaatgtc atgacttgtc atcctctgaa cgcgaaagtc atgacaaatc gtcttaacgg
    22201 agccacaagg catcaaatct catgaaaaaa caggaaatat tacccccccc ccccccgcat
    22261 tataacgcac taaatattag tgtattagga gataaatttt cagcaagaaa gggttacgtc
    22321 aatgtcgggt aacgtgatgc ttgtgttttt cttgctggct gtgctgttcg ggttcgtttg
    22381 tttcggcctt ggtattgtag agggttgccg gagaaccacg aagaggatct tggagaatat
    22441 agcaaaagga atgaagaagg gggaatagat cattccccct tcttctcgtt cgagtattca
    22501 tcttttttcg ctatatattt atattgctta tctagtcccg tacagagatc tattactttg
    22561 ctgcggagat aactttctaa atcaatgccg ggaaagtttt tctgaaaacc tctaactatc
    22621 tccatttctt ctggagagaa attcagcgat gaaagtagac ttttgtcatt ggaacattct
    22681 gaaatcgtag aaacgtcagt ctgtttcata agattttgaa tcactaaaat cgcatgagag
    22741 ggaacacgtc ctcgagaagt aaaccagcca tcaacggaga ctttctcaac tccacattta
    22801 tcagctagcc aaaatcgatc cttcccaatt cctttaagcc agtctttgat ctcttctttg
    22861 gtgggcatga ggaaaggata tgtatttatt acatgattgc aagcctttta ttgtgagcta
    22921 ttatgaaata aatacataat tattattgac aatcatgtag aaattacata tttttacctg
    22981 cgtaacgcaa cactctgaaa aaatatgacc aacacatcat catctcttcc tcctgatttt
    23041 tccttgcctc catcaatcat ggcaactgtc agacgattag ccaaagcttc aggaaaaacc
    23101 gaagaagttt ttttgaggca atatctcaca gaatgcatga aagaagaaaa tgaggcattg
    23161 gagaattata aaaaaatcag caaaacgcag ccgaataaaa ccgaaaccgc caaccgataa
    23221 atatggatta cttagctcag caagtgggga ttctttttga gataatccca ggcaacatcg
    23281 gtgatgttga cgtcaaaccc ggtcacaatg cttccataaa aggagtgccg aataagaatg
    23341 ccggctgcta tcagaccatt tacgatacca tccctatcat caaatctctg tgtgcgggta
    23401 tcgaagaaga tatatctgag aagaacatct ttctcttttg gagtgagagc atggagacgc
    23461 ttgatcacct ttttacggga ttttgcctcg gcgtaagagg atttgattat attccaaacc
    23521 ctttccagca gggcgcatcc ggaaaaaagg aacacgtacc ccgcaatatc gcggtaatca
    23581 tgatccgcat agctgatatg cagggcctca agaacgcttt gaggagcgaa aagataaaca
    23641 cctgttgcgg aaaagattat gcctgcataa ttgcaagcga atttaagcag ttctgccaag
    23701 tattggaaaa aaatcatgaa cgcagaggat agacttaaac ggaaataaag gcaatagcgt
    23761 taacatcaaa gataaacaag aaaaacacat gaagacaata taccttacca gcaaggaact
    23821 acgcaaggct ttgaaaatta gcccgggaac gctagttcaa tacaacaagg aaggttgccc
    23881 ccgcgtttat tttggaacgg ttgtgggagg aagtggaacg catgtgcgtt acaaacttga
    23941 cgatgtaacg gcatggcttg agaaaagaac tcaagaattt ttgaagaaag gaggcaagaa
    24001 gtgaaagctc tggccgccct cgtcttctcc tgccttgttc tgggaggttg taacccacgt
    24061 ccttatgact ggatttggaa aggggaacgg gaatggatta ttaagatcga acagccaggt
    24121 cttctcgata tttctttttc cactagcgac cctgagtgcg tgaaggcaat aagtgattat
    24181 aaaaaggata tttatggcta tctggtcact aaaccggacg gtgaggaact ctttatatcc
    24241 ggcaccgcga gaataacacg aaaggaggct aaatgatgac tgactggcaa tgcttggtag
    24301 cctgggcgtt cataacgacc tctctatcat tcgttatgaa ccttatcatg gtttttacaa
    24361 ggataacaaa agattaaccg tgaagaagaa atacccgaaa gtaataatta aaaacggtct
    24421 attggctatc gaagaagaac aatcaacaga aattaaagta atcatggata aggaaaaaag
    24481 catagttgaa cttctcatag agctggacaa ggttctcaag ctcgaaacga aggaggatgt
    24541 tcaaatgtat tatttatcta cggatccgga agataaaatt ctccggacga aatacgcagg
    24601 gagaatttac gtagcccgga ggcttttagg gttgagcctt gaagccattc ccggcgcatt
    24661 attgccctcc gagccagtag acgagttttc cgagttggtg aataaatcta aatcctccgg
    24721 caaccatgat cattgaatac gataacgaca acaagtgtat ccggataaat ggagaatacc
    24781 tccccatccg ggaagcggag ggcatcaagg acgagttgga attagcgatt gaccaatggg
    24841 aagttgacca cgcagagcag tgcgacaacc cggacggaca ctacgacgat taaacgactt
    24901 ttaaccaatc gcccggccca ggtggggcct aaaaccaaaa ccaaaataca aatcgaaacg
    24961 gaaaagagta atacggtctg gcaggcgcgg ggcggcgtaa agtcccgtcc gggcggccat
    25021 ttaacaatca acagaagcta aatatgggca attacacaaa aacaatgttg tgcttaaaaa
    25081 gggccttgga cgcacaagcg gacacaatca agacactttc ccaggcattg gatgagttga
    25141 cgaaaaatcc ggctctggaa tctcctgaaa aggatccagc tccttcatcc gatgcggatg
    25201 gcgtgaagta tgcccgggtt tccactctgg ccaaaagatt caatttcaaa actgaacagg
    25261 gcatcattcc tatccttcag cgcggagtga gggaaggcgc catcaagaag ttaggaggga
    25321 aaaagagtgg aatgataact actttcctgg tgtccgatgt agacgcctac atggaaaaaa
    25381 caagagacat ccaaaaatca tgactacgta tcaacacctg atcgaccgtg gcccctacaa
    25441 ggggatggtg gaaacgctca ccgacaaccc gcacccgtcc aagacgacgc gctgttacat
    25501 gtgcgccgag ccgctgaaag cctcgacttc atggatgtct ctggtagggg accatcagaa
    25561 cggcgtattc tgcgcccggt ttctgtgccc tttgtgcgcc agggaacgcc tcaacggcat
    25621 cccggacgac gcggaacgga gctggaacta cacccaagaa gccaagccca aagccttctg
    25681 gcttgaaagg ctctttgaaa agttcatggc cttttttgtg tggtctctga tcgtcttggc
    25741 aggaggattt atcttatttg tcttcttcct tctgattaaa acctttttta actgattaac
    25801 aaaataaata aaatggaatt taaagattgc actgttggaa caaaagtaca aatcaaaggc
    25861 acaatatgtg aagtatttga taaactgaaa accgtagcct tagaactaaa aggagggcat
    25921 aaatttcttt tcggttatga cttctttgaa cgggatgtta atcaatatga ctcgaagagg
    25981 aaattcagaa agggcgatat agtcaggccc gattacaagg gaagaaaatg ggctggcatg
    26041 ttggacgaaa gtatagatta caaagttcta gaagatgaaa gccacgatgg acaagtactc
    26101 attacgatag ataaaattgc aaaccccagc ggatggggga aggtggattt ttccaggttg
    26161 gttttgatta agccaataga gaaaattaaa gaggaagagc cttactatgt tgatgaatca
    26221 tgtgggtgct ttgatgttcg ccgtgaagat gagaataacg ataaatatat atgctccatc
    26281 tacttcggcg accacatcgg ggtaaccaga gacaaggccc tggagaaagc tgaagagctt
    26341 tgcgacgaaa aaaatcgcga gtaccgcgaa tccttgaaaa actgaaacaa aatagccggg
    26401 tcagcggcaa ctgacccagc ttaacaacaa taatatatat gaagaatatg aacactacta
    26461 acacagaaaa aacagctagt caagccccct gtccgcccaa gacaatcgcg cccatgctgg
    26521 tggagctggc tgaaaggatg ggcgtcaatc catcccaggc atacatgacc atcaaagcga
    26581 ccatcgcgcc caaagcgagc aacgaggaac tcatggcctt ttgtgtcgtc gccaaccagt
    26641 acaaactgaa tccattcctg aaggaaattt acgcattccc cggaaaaaac ggaggcattg
    26701 tccctatcgt cggcatcgac ggatggctga aactgatcaa cacccatcca aaatttgacg
    26761 gcatggatgt ggacatgagc gcagacgggg aagaatgcac ctgccggatc taccggaaag
    26821 acaaccagca cccgaccgtc attacggaat atttgagcga atgcaagaag aatacggagc
    26881 cttggcgtca atggccgcgg cgcatgctgc gccacaaggc catcatgcag tgcggtcgcg
    26941 tggctttcgg attcggagga atttatgacg aagacgaagg caaagacgtc gccggaggaa
    27001 tgcggaacgt aactccagaa aaaacagacg ggcccgccga aggggaaaca ccctggaaca
    27061 acgcgccctc cccggaagaa ttcaggagtg aaccacagga agccctgccg gaacccgaac
    27121 tgcaggacga ctttatcccg ggtttggaaa tcccggctgc agaagaatac acaaccacca
    27181 acatggagga ttattgacat gagcctgtct aaaaactgca tcgtttacga aaacatccac
    27241 cagcgttcgg atgcctggtt caaacttcgc tccggccgtc tgacagcaag caactttaaa
    27301 cggctcctga ctccaaccgg gaaaaagccc caacccaaaa cccaccagga gcgaggcccg
    27361 tggggagaac tcatcattga cctgtgctgc tctttcctcc gcccggatga aatcaagtgg
    27421 gaagggaacc ggcacacgga ccagggggaa gaactggagc ccgaagcacg tgacgaattc
    27481 agggacatca ccggaatgag cgtcaaagaa gtgggatttg tcctctgcga ggacgggccg
    27541 gtgggatgca gccccgacgg gctcatcgtc gaccagtccg gcgactatgt ggccggactg
    27601 gaaatcaagt gccctctctc caagactcac gccctctatc tgctcaacgg cgttctgcct
    27661 cccgaatatc gaccccaggt tcacggatcc atggcggtga cggggttgcg gacctggtat
    27721 ttcttttcct actgccgagg cttgcgcccc tttttgctca aagtggattg ggacgaatac
    27781 accgagcgaa tcaacgagat tctgaacgaa tttaagaggg aataccgtga tcaatttgat
    27841 gtgatcatgc ctcaaatccg cccggacgca gaagggaggg cggcatgatg accacattga
    27901 ccattacctt gccccatacg ccgggggaac tctcgcctaa cgccaagact cccctcacgc
    27961 agaggggggc cattgtggca aataagaaaa aggtgtctgc caaacaacgt gcaaggcaca
    28021 tggcatgggc tgttacctat aaagcattac agggccagaa gtttatgccg aatttttata
    28081 ctgtccgctg gttttacaag gggccggaac cggatgatga caatgtcctc acccgctgca
    28141 aatactacaa ggacggggct tgctccgcca tgggtattga tgacggtaag cttcgatgcc
    28201 ggggtattga aaggattcat gacctagagc gcgccgggaa ggtggaaatc gtatttgaaa
    28261 ggagagagca atgattaaca tccttatatc cgtcaggcgg cctttctccg ggaaaattct
    28321 gtccggcgaa aagagatggg agttgcggaa aaacgtaccg cgcttaaaaa aaggcgactc
    28381 cgtaacgctg tggctttatg agtctggcat ggatgggaca cgggacatca tcggcaagtg
    28441 ccgtttagtt gtcactgctg gacttcaacc atatcccccc aagggaattt tagaatggac
    28501 catgaagcaa gcttgcgtaa cggaagaaca cattcttcat tatactcctt gcttcgtctg
    28561 gggcgttcag gatcccgtgc ggctccctca tgctgtacaa ctctctgcca ttggactgac
    28621 ccgtccgccg cagagctggc agtatcttac cccggaacag gcatctattt tggaaaggag
    28681 ggcgagtgaa tgagctacat cttttcgcgg gtgctggtgg aggcatactt ggcagcgaac
    28741 tgctcggaat ccgcaccatc tgcgccgtcg aactcgaacc ctatcccgca agcgtactgc
    28801 tcgcccggca aaatgacggc cttctcccgc ctttcccggt ttgggatgac gtatgcacct
    28861 ttgacggacg accatggcgc ggtattgttg acgtggtatc tggaggtttt ccttgtcagg
    28921 acatcagcgt ttccggaaag agaaaaggac tggaaggggc gagaagcgga ttgtggtcag
    28981 aaatggcgcg aatcatttgc gaagttcgac cgggattcgt cttcctggaa aattccccaa
    29041 accttaccag gttgggactt gaccgtgtcc tcggaaacct ggccgaaatg gggtacgatg
    29101 cggaatggtg tgtgttggga gctgacgcca ttggactacc tcatcatcgc gccagattat
    29161 ggcttcttgc ccacgccgcg ggcctgcatc ggatcacacg ggatagcgtg gggcagagca
    29221 gaacggggcg agcatcggag caacctggaa gattacctgg catatctcta tgtcaaaaac
    29281 ggtgggaagc gagtcagggg catgtgcgtg tcagcgtctt tcgccgccct gatgatggga
    29341 tggccgcaga agtggacgag cttgaagcca ttggcaacgg acaagttccg ggagtggcgg
    29401 caactgcatt ccgggttctg ctcagcagat ttcagcatgg aaaggataac aagtgaacac
    29461 cttcaataca cccaaaaccg agacgaccac taatgtgtgg ctcactccgc gctacgtcct
    29521 ggatctgctg ggtcagtttg acacggaccc ctgcgccgct acggtgcgcc cctgggattg
    29581 tgcccgtatc aactacaccg tggaggacaa cggccttctg atgccctggg aggggcgcgt
    29641 gtggttgaac cctccctatg gtaaagaggc cgaggcgttc atggagcgta tgagtatgca
    29701 ccatggcgga gggctggcac tcattttcat gaggtcggac acacgctggt tccagcggtg
    29761 cgtgctggac tgcgccaggt atctgttcct ctggaaaggc cgcatccgct tttgtcgccc
    29821 ggacggagaa acgcacggga accaacccaa cgcgccgagt tgtcttgtgg cgtggaacaa
    29881 cacggaagcg cccctcctgc acacgttgca gaatcagggg tatggaaagg tggctgtgct
    29941 atgaactaca acccccaact gacgcttttt tgatatgccc aatagaataa tcagagaagg
    30001 aatcatcacc agcgaagcgg tcaattccct gagctgggaa gccgaaatat tttatcgccg
    30061 cttgctctct gttgtggacg atttcgggcg tttcgatgcc cgaccgtctg tcctgcgttc
    30121 tgccctgtat cccctaaagc tcgactctat gagggaggat tccgttcaac gttgcctcaa
    30181 atcctgtgag gtggcccggc tcgtcgtcct gtactccgtc aatggaaagg aatatctgga
    30241 agtgaccaac ttccgccagc aggtgcggag caagaagagc aaataccctg cacctgatga
    30301 acatatgcgt agcaggtgca cagtagatgc taatcatgtt ataagcaaaa ttgaagaaaa
    30361 taactctatc agagagttac aagaattgca tggcacatgc gcatcaaatg cacagcatat
    30421 gcacactaag acggagacga agacggagac ggaaacgaat aaacaagaaa ataatgcaga
    30481 gggagataac acggtggttt gtagcgagcc gcccactgct cctgcgctgc ctgcctctcc
    30541 tttcccggat cgggaacgtt tgaacgacgt tcgggggatg cgctacgccg acaaccacgc
    30601 ggacctggga tcttctccag ccgctgcccg gttcgtggca gcgtgcctgg acatcaatcc
    30661 gtcctggggc cggacgatac caaccgccat tgagacggcg gccgcgcttg aggcgtaccg
    30721 gtccgcgcag ggccgggtga cgccgcggga tatggagatg ctcaaggctt actacgccag
    30781 cagcttgacc catgaccgga gcaataaggc tttttggcgt ccggacagcc gacggaagtt
    30841 ctgggagtgc tttggagatg tgctcactca cgcagatcgc tgggccaaag aaacacgctg
    30901 gaagccggca gcagtccgca agaagccgaa gcccgaagaa ccacggcagc cggaagggcc
    30961 cgttgtggat gtcgcggacg ctgcggcgga gatcgcatct ctgcgggagg aaatgggact
    31021 ggggggatga aaaccccctg gaatctcgta taacccttgg aatctcgtat agacctattc
    31081 ctccgacaat ccgtgacgga cgattaactc catcaacagc tgcatcgatg ggctcgtttt
    31141 cctcccgcct ctcaaccatt gccaaaccgc catttccgtt accctcaaca tctgcgcagc
    31201 ctttttgact gcaccggttt tggaggtgat taaagggtca tgggtttgga tccaatcaac
    31261 aaattcttga ggattcatat tttttcactc gcttcgccca tttaatctgc agattatttg
    31321 atttgctcaa atcgtatccc accagggtta tatctgacga cgtcccaacg cagataacca
    31381 tcgctgtcaa ttacttcgtg agcgtaatcg tgccagtatt tccggatggc atttattgtt
    31441 tccgtttcgg ttttgtaggt ataatagcac gggttttcat tcacgttttt gatgtagtat
    31501 gctgccagga ccaccgcaaa ttggtgacct tccggaaatt caatgatgcg gtcatacttg
    31561 gtannnnnnn nnngtagagg agatatccga taaagacgta tctccaaact tttgagggat
    31621 aatatatttt ctcatgtgtt tggtttgtgt ttaaaagttt gtgtttaaaa ttttcctccg
    31681 gtcagatcaa cgttcccgcg agcccatctg aaatcggcgc attccaaaat ggctccaccg
    31741 aatctgactc cggtcagatt ggccttgtag aaagtggccc atttcagctt agcctcagta
    31801 aaatcggcat cttgcagatt ggccccggtg aagtcggcac aagtcaggat ggccccggta
    31861 aagtcaagtc ccgaacagtc ggtgtcctta aagtcagcct tgattaagtt agcgccccta
    31921 aagtcgagac ccctgaggtc gaggccttca aatttggcct gagcgccgct gtattcgtca
    31981 tccagccaca tttcatgacg attgaggatt tcgtttaatt cctgctgggt cattgtatcg
    32041 gtctttctgg tttgggattg gattatgtta agctttgatg tcagtcatat tggcatcggt
    32101 aaagtcggca tcggtcgtga tggcgccggt caggtcagcc cggtacaagc aagcatccgc
    32161 cagaatagcg ccagtcaggt cagccccata caggctggct tcgcacaaat tgactccgct
    32221 cagattggcg cctctcaggt tggtgtggca caggaaagtc tggagcatgt taactccccg
    32281 gaaatccata ccgctcaaat ccatgccgcg caggtcaacc agccacaggt tcgctctaac
    32341 gccgccttct tctccgttca tccacatttt gtgaagcttg aggatttcgt ttaattcgtc
    32401 tttagtcatt gttttgttct ttctgatttg gattgtgttg tcgtttggtc ccttacctcc
    32461 cgtcaacaag atcaatatac taaacaatgt ttagtagcgc aagagaaaaa atgaaaaaag
    32521 gagaattttt tttcgttgtt ggatcccata aaaagtgcta acgaagaaat gttctttctg
    32581 aacgttcagc ccttttgggt tatggattaa aacgatcgca agatcattcg ggcggcaacg
    32641 cctatttccc ttaaaaagtg gcggttcttc ggagccgcca ctttttttta ctcgggaacc
    32701 gctggaacca gcataacaaa aacaaacaat gcgcttgctt tcggcgctct ctatctccta
    32761 tggtagagcc gcaggaaaaa gaaagcatca ccaacaacta aaagaaagcc ggggaggccg
    32821 agcctctaca ccgaggattt agcccaggag atagccgtgc ggctcgccaa tggcgaaacc
    32881 atgaaagcca tctgcgccga caaccacatg ccggacgtct ggacagtctg gcactggcgg
    32941 gaaaccaggc cggagttttc ccaacttatt caacgcgcgc gggaagctca gtcggaagcc
    33001 atgcttgacg cctgtcagga gctggccgac gaggccgcga aagtcgccct cgacccggaa
    33061 tgcggctccg ccgccgtcgc cgccaaaaag ctggccatcg aaacgcggtt gaaagtcgcc
    33121 gcccgattcg cgccggaaaa attcggcgac cgggtccgtc aggatgtcgc cggcgttccc
    33181 ggcgcgccgc tggaacggaa aatcaccctg gaccccgagc agctggccca gctgcaggaa
    33241 gacgagaaaa ccgcgctgga aaccatcgcc ggcaaactcc acgcttaaca gaccaggaca
    33301 cgcctccccg tcagcttctt cctccgccat gtcctccgcc tggatcccta tccctggcag
    33361 gtggaggcca tcaaggcgtt gtccctcggc aagctgaccc tgggagggaa aagcgtggct
    33421 ctggtcgccc ccaacggatc cggcaagacg agcaactgca tcgctccggc catcctgtac
    33481 ttcctcacct acttcccgcg ggggcaggtt ccggtcacgt catcgtcgtg gatgcaggtg
    33541 gaaaagcagc tctttcccgc gctccgccgc tacatggaca accctttctt tgacggctgg
    33601 accttcaaca aaaccgagat tcgcacgccg gagggaggct ttgccgtggg cttctcgacc
    33661 gacaacgccg gccgcgcgga aggatggcac ccgaaaatct cgcccgacgt ggaccccgtc
    33721 ttttacgtcc tcgacgaggc taaaaccatc ccggactcca tcttcaccgc cgtttcccgc
    33781 tgcacgctct tccacgcgtt cattacctcg tcacctgggg ccgattccgg caccttctac
    33841 gactgcttcc acaaaaattc atccctctac tacaagatcc gggtcaaata cgaggattgc
    33901 ccacacattg agatcaatga cccgggcaag gccgagcgcc tgaaaaaaga atacggcgag
    33961 cagtcctcct tctaccgctc cgccatcctc ggcgaattca cggacctcga cggtcagtcc
    34021 gtcattcccc ggcgcgccct catggatctg gtcaacaacc cgcctccctt tctggacacc
    34081 ggggagacct gcggcggctt cgacttcgcg gccggaggcg acgaaaacgt cttcgcggcc
    34141 gggcagggca accgattctt catcgccgac cactggtcgg atccggacac cgtaggagca
    34201 cgcggacggt tccgccgccg tgcggccgaa ctcggcatcc ccgccgaccg catttacgcg
    34261 gacggagacg gcctaggact ccccatcatt gacgacttcc gcgccgaggg cttcccggtg
    34321 cattcctacc ggggcggctt cccggctgac gatacgcagg cctttgtcaa cctccgcgcc
    34381 caggcgtggc gggccctggc acgcgccatc gaagaaaaag agctcatcct cgacatcgac
    34441 gaggacacgg ttgagcagct ggtagcgccc cggcttcaaa ccgacgcgat cggccgcgtc
    34501 cgcattgaga gcaaggaaga catggcgaaa cggggcgtcc gttctcccga ccgcgccgac
    34561 gccctcgtca tggcctggca cgcgcgccgg cacagcggac tggcccggac gctgggagcc
    34621 tggtactcgc gccccgtgtc atccaaacga tccatcggga gatattaggg ttgacaacat
    34681 atcaacatat ccgtatatga cgatatgtaa tcaatcgcag ggtggtgaaa cggtatcacg
    34741 cggggttcct gtccccgagt aggaggtcca attcctcccc ctgcaaccac cattttcttt
    34801 ctcttccggc tcaggttcaa cgcattaaaa aacatcctca acgccccgaa gctggtcgcc
    34861 caacaggaga ccagaatcaa ggagctggag acagacctcg cccggcgagc cctgacggaa
    34921 caaagccggc agcccaaccg tccccaatgg tacgagtact gggatccgct gcagggggcc
    34981 gacttgagca ccctggtgga ggcccgaaac gaagcccggc ggggagcctt tgcccggcaa
    35041 atgctgatct gggacgaaat catttattcg gacggccttc tgggcatgct ctactcccgc
    35101 ttgatcgaaa gcgtcgccat gcagggctgg aagattgacg ctgcggacga cagcccggag
    35161 gcccagcgcc agaaaaacgc cctggaagaa ttttatcact ccatcaacgg gctgcagcag
    35221 tcttttggac agctggcttc cgccatgttc tacggttacg cccatctcca atacatcgag
    35281 gattcctggg gccgccgatt tgaattcatc ccgcagcgct attgggtacg gccgggagag
    35341 ctgaatcaat ggcagttcaa cccccagtgc tacatcgggg tcgatactgg cgaaagcgtg
    35401 gaagacgaaa cgctcgtcgt gatggagcat cggtatccca tcctgttccc ggctgcccgc
    35461 gcctcctttg agcgaaacca tgcaaagata acatgggaca accacatgga ccgctacgga
    35521 agcgccccgg tcatcatcac cgccccaaag gacgcgagcg ccgccgtcat ggacgcgctg
    35581 gaacgggcct gcgagcagct taaatccgga gcctccatcg tgctgcctcc tgactgcaag
    35641 gcggagccgc tcaaggcgtc caacatcaac gaaaactatt ttctttcgcg catcaacatg
    35701 gccgacaagg atcaggtgcg ttttgtcatg gccggaactc tgaccgtcct gaacgaatca
    35761 ggatccggca cgctggccgg gtccgcccat acggacagct ggaatgcggt ggtatccgcc
    35821 gtctgctcta aagtggcgga agcattcaac accgccatca gcccgcttgt cttgggagac
    35881 ggcgaaccgc tggcccgcct ccacatcacc tttgataccg tccagacgcc attgcagaag
    35941 gccgaggaaa tcgccgccct tgcggacgga ggcgtccgtc cggagaaaac cgaaatcgaa
    36001 gaaaaaatcg gcatgtcaat cgaggactca agggagcccg tgccgacggc ggcggcagcc
    36061 aacagggatc cgggagcctc cctcattccg cccgacgcct acgaacagct gcagcagatg
    36121 atttacgccg gcctcatgaa aggatttacc gatgatcagt accagacaaa tcaatgacct
    36181 gtccaggccc gccaacggct ggttccacgt tgaaaaaagc ggggaccatg acgtcgacta
    36241 cggcgaaggc cccgccgtgc tgcgcatcga cgagcaggcg atcagggaca tggtggacga
    36301 cttcaacgcc cgcacctttg acggcccagg catgctcatc gacggcgacc acctgagcca
    36361 cgacctttcc cgcgatacgc gggcgctcgg atggctcaag aagctggaca cctaccgaga
    36421 cccttccggc acgctggaac tgtacgggtt cgttgaatgg actccgcgcg gcctgaagat
    36481 gctcgaggac aaagagtaca cgcaatcctc caccgaatac ggcgacggga tgtccttcaa
    36541 ggacggcgtt taccgcccgt cacggctgac cggattcgcc ctgaccaacc gcccccgcat
    36601 caagggcaag cggccgctgg tcaatcgaca gacttccccc gcctctgacg aggccggggg
    36661 cgacaccaaa agccccaccg aagaggggga aacaaaccag aaaaccaata tggacaacga
    36721 cgatagagaa tatccgtcca aggaaatgga caaggcccag cgggccctgt ttgattccct
    36781 gcttgacaag ctggatgtcg aatttgacgg caccgacgac atgagcaggg ctatcctcgg
    36841 acgccttgac gaactgctct cgctggaaaa gcgtgagaaa gaccacgtga acgccgaagt
    36901 ggacgacgcc gtcagcacgt acgaaaacgc gctggacgaa gaagaacgcg aggaattcac
    36961 ggaagaacgc cgggaagagc tgaaaaactc tctccgggaa agccccgccg cgctggacgc
    37021 ctttgtcagg gcgctcaacc gccagactca acccagtaac aaagaggaga cggaaaagaa
    37081 agagccgccg aaaaggcagc ccctgaaccg ccgcgttacc ctgacgcctc ccaatccgtt
    37141 ccgcaaaaag gaatccatcg acggattcca aaaccgcgtg aacgaactga tgaagggagg
    37201 catggaacgc tacgaagcct tccagaaagc cactgaggaa ggatacatcg tcacctccga
    37261 acgataacca tcaacctgat cacaaccaat gccatcaatc aatatcaccc aaacggacgc
    37321 cgaggtctac ttcaacgcgc cggaaggcgt tgacctgtcc acgctggaag gatccgtcgt
    37381 atcgctgacc gggaacccgg ctatcccaga gcttgtcggc aacccgctga cggctcttcc
    37441 cacgcagacg cagctgctcg gacttgtcac gcaaatccaa cccgaccggg gcacctgctg
    37501 cgccgttctg gtcggcatgt atgccggact ggtcaaggca tctcttgccg agactcccgg
    37561 aaccatcagc gtagggacgc ctgtgactat taccgccaac gggacctgga aggccgccgc
    37621 cagcggcgac accgtctatg cccgtgtcat tcatgaaccg tgggaaccgg gaaaaataga
    37681 aatcgggttc gtcgctccat accaggttgc ggccgcctga ccatcacctc taaccattag
    37741 aaagaccaac aacaagggct aatctatttt attcagctgt tcagtttacc gatgtcctga
    37801 ccgccttttc cgtgggagcg ggaaatacgg aacgggactc catgatcaac cgcatcgctc
    37861 cgctcgtccc ggtatacgat atttccttcc agtatatggt atgggacacg ccggccgcgt
    37921 ttacggtgga acccatccag gtggcgcccg gtgaacctcc ccgccagact tccatgctcg
    37981 gacggaccga aaccgatacc ctgcaggggt acgcgcttac ggatccgatt cccgatatta
    38041 tgctgggggt caacagggaa aaggcgcagg cgattctgct cgcccatgcc cgcttcctgg
    38101 aatcgaaatt cgtcacatct tacgaatatc agcgcgccaa gctcatcgag agccaggttc
    38161 cggcagcgtc cggttatgga gattgggaca atgcgcagaa aaatccgctg acggatttgg
    38221 acaacgcgat ccgcaccatc aatgccactg ccgggaaaat gcccaatacg attgttttcg
    38281 gcgccaatgc ctgggcccgg cttcggtcga acccgctggc aaggcaggta gtgtcgtaca
    38341 acagtgtcgg cctgttcgac gaagatctgc tgaacagatc gctgtatacg cggatgaagg
    38401 aagtctacgt caacaacatg cctttctacg acccctccgg tcaagggaag acaatgatgg
    38461 aagacgacgt ctacatcctc tacaaagaag attctccgac gcagtttgac gcctcggcta
    38521 tcaagacctt cgggctgaac ggacagttcc gccgggaagt aacgacggaa tacttcccga
    38581 ccaacaagga aacaaaggtg accaaccggg tttactcgtt gaccaagctg accaaccccg
    38641 gcgcgattat ccgcatcaac acggcgtccg ccgattaacc ccaacgcccg cctccatcat
    38701 gtccgccttt cccgcctggt ccacaatctc cacggatgaa gccgaccgcc ttctcggcct
    38761 caataccatg gagcgcaacg ccctggtgaa ggccggggag cagcgcagcc tggactacca
    38821 gggtgtcatg atggaggcgg tcaacgatgt ctgcatgacc atccgcgggg cgctggccaa
    38881 caacctcgcc ctgcggcaat cgctccaaaa cagcggcatg tacgacattc cgcagagcat
    38941 gcgcgccctg gcatggccga tgatcatccg gcagctctac ctgcgctacc agctcaacct
    39001 gaccgaaacg cgccagaaag ccgccgaatc ggccgacgcg atgctggctc tctatgcccg
    39061 gggcgacatg ctgcccgaaa gcgtggacgg atccgctccc gcggaccccg cctacatgat
    39121 gccgcgcttc acgaaacgac cttggttcaa cccaatgcga agcacctacc gatgatgacc
    39181 gccgcccaga gagagatgat cgacaacgac tacgccgaac gcgccttttt cgtgtccggc
    39241 gtggaacccg gcgtcatcct gtccgatttc gaggacaagg ccgcgaaagt cgcgtccggc
    39301 gccttgagct acgaggaagc gcagcaggcc atccgcgaaa ccctgcgcca gcagggctac
    39361 cgtcccccgg cgacaggtca gggaggcatt cgggatttgt cctcctgggt ccgcatccag
    39421 gtcgtgatgg aaaccaacgc ggccatggcc cacggatacc ggaactggta caactggacg
    39481 caggatgaag acacggccgc cttcaaattt taccgctccc agggcaggga agacccgcgc
    39541 tattgggccg aacgctggaa ccgcgccaga gcggggctgg aagaagaagc cacggaggcg
    39601 gtatcctccg gattcatccg gggcgagacc gtcggctatg ccctggcgtc ctccgatatc
    39661 tggatccgtc tgtcgcggtt cggcacacct taccctccct ttgactacct gtccggaatg
    39721 aacattgctc ccatcggagc cgaagaagcc cgcgctgccg ggctggacgt ttcccgcgtc
    39781 cgtcccgctc ctgccagctt caacgccaca ctggaaagca atgcccaggg tgtgacggaa
    39841 gccaacaaga agaagatccg cggcatcctg aaagacgccc tgcgcgtcaa caacgaaaac
    39901 gacggcaata ccacctttgt ctataccgac ccaaacggca cgcggcctta cacggacgcg
    39961 gaactggcgg aactcctgtc cggggatttc ccggaagaga tccccttgcg gcaggcccat
    40021 gccttccgcc tggcggcagc cgggggagcg gtggccggaa cgctggcatc cctctacctg
    40081 gaccgcctgc tggaccgcct gttttccgag ccggaaggcg tctggttcgc ccggcccgcg
    40141 gatgtggctg ccgcgtcgtc ccgccagttc atccccgttt cccggaagga agagggggaa
    40201 ttcacctggc gccttgcctc cgggcacgtc aaaaaagtgg aagacgtcgc cggagctctc
    40261 cgcgtggaac tgccaacccc ttatgttttg cccgtcaaat ggctgtaacc gtccatatcg
    40321 accagaccgc gattgaccgg gcgtttgccg agatggagcc gtccgcggcc cgtcacaaaa
    40381 cggccatccg caaagcgggc gtcgccctca gcctgcttat tcaggaaacc ctgcgccagc
    40441 agggcaagga ctactacgac ggcgcggcgg acgccacctc cacggatgaa accgccgagg
    40501 gcgtcagcgt ctccatcgcc tggcgcggca tcggcctgca ctggctcggc acgcagggct
    40561 atctgggcgg cccgctccgg cccacggggc gcacctcgga aatcacgggg cagccgatcc
    40621 aaaacctcgc gattcccacc atcaacgccc cgcgcgggca tggaggggcc cggagcattt
    40681 acagcgccgg gttccgcaaa gacgatttgc aattcattcc ctccaaaaac ggaggacgca
    40741 acggcaatgt gaccggcgtc ctcatcctca agacggcgca gtcgtccacc ggaaagaaag
    40801 cggcccggaa gctgttccgc aaaggagcca aaaccgggga cgtcctctac gtgctttgcc
    40861 gcgaagtcac gatcccgccc acgccgggga tcctcccgac gctggaccgg atggcgcagc
    40921 gcgccgcgga aacctacctc gccaacatcg gaaacgaatc atgatcccct cccttgacca
    40981 aaccatgtgc cggcgcatca ttgagcgcct gcagagcctc ggaacattgg actgccatat
    41041 ctttgagcgg ccctttgacc cccagtacgc cgccaatgac atcatcatgt ctgcgatggg
    41101 aaacaacggc gtggtgctgg tgtgtccggg agacgcggac gagtaccagg acgggcacgg
    41161 gcagacggaa gcgccgacca tgtggcggca gtatttcatt attgccgccg tctaccacaa
    41221 cgcggccctg ttccccccgg aatgcctgac gcccgactac tacctgcggg cggtaggcga
    41281 cgaaattgaa gaagccctgt ggaactggaa cccttttccc tttgccgcgc ctgcgatgat
    41341 gaaaccgaaa atcaaaggcc gtttttcctc ttccgccgtc atcgacggcg agaagcggca
    41401 aatgaacgtt ttgaccgtgg attaccgcgt cccggtcaat attaacatca gaaacaaccc
    41461 gcaattccaa tgagcagaag agcaacaacc aaaaagaagg ccaccaggca agtcccggcg
    41521 gaacccgaat cagccgggga atctccggac caaacgccgg caacgccgga tgaactccgg
    41581 gaatccgacg atgaaccatc gtccacgccc gacaagaagt ccgtccgggt cattcgcacc
    41641 cgtgcggaac tggacggggg gctggtcatc agcctctcca tgaaaaccga taccccggaa
    41701 cttcccgcgc ccgtcgcgga agctctgcaa accctcaacc ttgttgacat caaatgagca
    41761 aagcaactgc cgccaataca gaaccggaca aaaagacgga gcaggccgcc gtcatcaaca
    41821 cgaacatcct cattctcgcc aaagaagtgc gcatagggcg ttcaacgttc ttgaaaggag
    41881 cgcatattcg tgttacgaaa gaactcgcca ataaactgga agccgacggc aaggcatcca
    41941 tcatctacta accatttcaa gcatcagggc tactacatac gaccccactt ttagcaaccg
    42001 caacgtgacg ccgcagatta ccggcgttct ggctattttc cttcccgacg gcatcaaggt
    42061 gaccgaggac gagggagctt cttatgtcac cgggccggac cagtttccga cgcctccgac
    42121 atctccgccg acggatccga ccgccgggcc ggaagcgccc tgggtgagct tcgggttact
    42181 gggtgccttc cagtccgtcg ccacgcaagt cgaaggggaa gtgacgcgct tttacggcgg
    42241 cgcgctgggc taccgccagc agcgcaagaa cacgacgacc ggcaagcgca tgaccttcac
    42301 cacgccggac atgtcccccg aatggttcca gctgtctttc gctctgggag cgcctcccgc
    42361 caatggagag gaatcgacta ctgtcggaca tggcggcgac aacaagattg aggggtatct
    42421 tcacttctgg taccagaacg acgtcggcac gatctatctg gtcggaacgg cgcatggcgc
    42481 gttgcgcctc cttcaggatc cggaacatac cacggcgatc gcttcacccc agttcgagtt
    42541 cgagatggat taccgcggca agtaccagtt cacgccttcc aatgtgcagg acgtgacgcc
    42601 gacgccgggt tcctgacgtg tttcaccagg gggcggatga cgcccccgca tccttgtttt
    42661 tttcaggcag caggcaggta aatatgatat caggaccgtc acggggctga accagtccct
    42721 ggtcgtccgc gtcgtggatt tccagggcga ctccgtcgat atgagcggcg tcaccctgcg
    42781 cggagccgtc cgcctcaaga cgggcgttac ggagttcgga ttttcccgag acgacgaggg
    42841 aaacggcgtg atttcctggg agtcggtgcc tgcgggcatg tggtcctatg acgtcttcat
    42901 ggacgacgga agcgaagaaa gcccccttct ctacggttgc tttgtttctt cgggccgggt
    42961 aacgccggac ttgcccggcg agcagcaggc cgtggcgggc gcggtcgtcg tgcagctgcc
    43021 ggaaggaagc ggctgcgtgc aggtggtgct tgataatgcg tccagcgccg cctggtacgc
    43081 tgagcaggcc aagaagtacg cggagaattt tagcctgtcc gttgacgaag tcaccactgg
    43141 ggaaccgggg acgcccgccg ccgcagaagc cgtcaaaggg gaggctgccg gctcctataa
    43201 gctctccttc accattcccc gcggggatgt cggtcccgaa gggccggcag gcccgcaggg
    43261 agaacggggc gaaactggac ccgaagggcc gcagggcccc cgcggcgaaa ccggggaacg
    43321 ggggccgcag ggcgaaacgg gcgagcaggg cccgaaggga gatacgggcc cggccggccc
    43381 gcaaggcccc gaaggcccgc aggggccgga aggtccggtg gggccccagg gacctcaagg
    43441 ggaaaaggga gatcccggcg acgtcaatac ggaagaatcg tatacgtgga gccagcccca
    43501 ggcgttttca agcgccatca acgccaatgg aggcgtcaac attccgctgg ccgtgggagc
    43561 gccgaccaat gaatccggcg tcaaccgcct gtatgccgcg ggcatgggag gcgcagccaa
    43621 tgcttatgcg gcggcggctt tcctgaatac ggactcgctg acaactaccg gcacggcaac
    43681 ggtgactaaa acggttccct accagatggc cagaataggc attcccgcgg gcgctcacac
    43741 gactattcag gccccctttg aaggccccag ttcccaatgg aattattctt cctgggccgg
    43801 cttttcattt gtctggcggg ccacggccgc ggcaaaggtg acgataggca tcggccgggg
    43861 ggcgaaaaca atcaggacgg accttaccac cgactcctac acgattattc cgggcaatga
    43921 cctggcattt aattccgggg agattctgga catcaccttt gacaacgtgc gcgatacgac
    43981 gcgcaacggc tatacggtgc gcgtgcgtga aatttatgcg ctgacgtccg ccgatggatg
    44041 gcaggtcaag acaacgacca gctttatccc ggcgacgcaa aacgagcccg tcccgtggac
    44101 ggtctgcaag attatttacc agcagcagac ggttgccaac agcagcgttt atgaaaacct
    44161 gggatgcctc tggctgctgg tcacgggggc acagaccaac aacctgttca agatcgccac
    44221 ctgccggggc gtctccaatt ttgagaccgg cgtcggcatt tccagatggg tgacggatgt
    44281 gatcaacacc accagcggca cggcttccgt ttacgccggg cccggagaat atgcctacta
    44341 ccagccgggc aatattaacc ccctctttta cgggttggat gcgataggaa cgaatgccgt
    44401 tgaatccgag gcaaccgccg cttttgaaga tattaacgta ccaatggaat gaacagcgca
    44461 gaaatacaaa tacagttccc gaaacccggt caatgggatg aattcggcct ggcgtccatt
    44521 taccaggacg cggaagggta cacgcgggca gacatctata cagcggacga gattccagcg
    44581 gagcaggctc cggccatgca ggccgtcgtt gtcgcgctgg tgggattgtc cgagccgtgg
    44641 caggcctccc aggtttgggc gcggctgaaa gagttttacg ttccggaggt ggatgacccg
    44701 atgcggacga cggaaaccgt ggatttgacc gtggaggccg tcaatccgca gggagggcgc
    44761 agggtgttca cttcccgcga ctacccggct tttatgatca cggatcccgc cgccgtggac
    44821 ttttttaaat acttcacaaa gcaaaaccat gagtaagtta agtgacgagc aaaagaaatc
    44881 tgctcttttg gagtggatag aacaaaaaaa taacaaagac aatgactaac aacaatcaat
    44941 gtaatcatgc cgaggcaata gccaaggatt tttatgcggt agtttccgag gataacggcg
    45001 gcggctggaa gccctgggaa gaattaaccg agccacagcg ggcagcgttt gtgcgattgg
    45061 cacagcaagc ccttcccatt atcggcaggc atacgctggg ggatgtccgc aactaccttt
    45121 tagggcaggc caagacctcc accggttggg aaaaatggct ttactgggcc ggggtaggaa
    45181 tcgcagggat cgtccttggt gccctgggaa tgtccctctc cggctgcggc cattccgtgg
    45241 acgtaacgcc ggacaagacg gtggtctgca aggacggctc ctgcctggtg ctggaaccgg
    45301 gccatatctc ctattcacag gcgcaacccg aaacggacgt tccgcccgtt gtgcaggtaa
    45361 ttccctccaa gaagtaacac catgtgcaaa ctctccgaag taccggcgcg tttcatggat
    45421 tttgccaagg cgtcacccgt gtttgcctgc gtcatgctgt cgctggtcat ttgcggcgcg
    45481 tcctgctggt acatcgggga tgtcatggga caccataatg accggctgtg tgacctgatg
    45541 accatgcaga cacaggccca ggtcgagacg gccaaggcga tccaacttct tgccgtccgg
    45601 attgaaaaca tagaaagaaa actggaaaaa tgaataagct gttaaaccct tcccttttgc
    45661 ttccgttgac agggtgcgcg atgggccttg cttttgccgc attcggcgac acggtggcag
    45721 gactcgtagc gttctgtttt cccctcgcag tactgttgtt ccttcgagtt catgaaggct
    45781 gatcaactgt aaagtttttc ttgccagttc aatcatatta acaaccaacc attaaaggag
    45841 aatactcatg aaaatagcca ttgatattgg ccatgccaac aacaccggag cccgcgggaa
    45901 cgggcttgaa gaacacgccg tcgccgtgac gatcgccgaa tgccttgccc ccatgcttca
    45961 aaaactgggc gccaaggtgg acgtgattga ctttcctggc atgagcaatg cccaggattt
    46021 gaacgccacc atcaaggccg ccaatgaagg cggctatgac ttcggcatct ccctgcattg
    46081 cgacgcttcc gacaatcccc aggcccatgg cgcccatgta tgtttttacc cgggcagcgt
    46141 caagggcagc aggctggcca tgtgcatcgc ggaacctctt tcccgcctgc ttcccggccg
    46201 ggccaatacc gtgcagtcgc gcccgggcct tgccgtcctg aaaaggaccc gctgcccgtg
    46261 ggtgctatgc gaatgcggct ttatcaccaa tcctgaaaac gccgccctga tgaaggacca
    46321 tcccggacgc attgccgaag ccattgccga aggggtgaag gactacctca accagtaacc
    46381 gcccatgacc taccaggccc cctacgcagc ccgctacgtt tccgccgccg gcaaccagat
    46441 ccagctgctc aacctctggg acgatacgcc ggagccgacc cgcttcggcg gttccatgga
    46501 agcatttgaa acgtcgctgg tagacggccc cagggcgttc gcgcaggggc ttggaagcgc
    46561 cgtggagcag cgcaccatcg cgttttaccg ctggttcgtc gattaccaag atatggccac
    46621 ctggcaggag aacatggctc tgtggctggc cagcaaccag aacggctatc tctacctgca
    46681 gttcgccgaa cagccgcaat ggcggtttgc cgccgtcatc accggctacc agtttgaaac
    46741 cgagaacttc gttccgcctc cgtctcctga ggacggctat ctgtgcctgc tggtcacgct
    46801 gaccttgacc gtcaccgaca ggagaccaga caattccaac tgggtttttg ccgtgacgcc
    46861 cgcttctttt gacgtgcccg tccagggggg tgaatatacc gtgaacgtgg aatcttcgtt
    46921 taccccgggt ccggtcgggc aggggtggca gatagccgac gtctccgaag ggttgaccgt
    46981 ctccgatgtc gtcaacggca acaacggatc attcaaggtg gcagtctccg ccaannnnnn
    47041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctccg atgtcgtcaa cggcaacaac
    47161 ggatcattca aggtggcagt ctccgccaat gaaggagcgt cgggaacagt acgtatcacc
    47221 gcgacacaac aggactccgg ggaaaaagcg taccttagag tgctgcgcgc gggactggtc
    47281 acgcgcaatt atacgctccc acctcccaag ggagagtata cggatgtccc gatgggatac
    47341 agttcttggc ggttcatccc ctcggacgtt tatcccgatt tcccggcagg caacccggaa
    47401 ggaaagggaa tcacgctgca ggaaatcgtg acgacgaatc ctacgagcgc caattcaggc
    47461 acgttgtcgc tttaccgggt ggaaaatgga acggcttctc ttctggcgac cagcaacgag
    47521 gctgtttcca ccggagaggg cggaaatgtg aaatggacgt tttctccagg cgtggaaatt
    47581 cgttcggact ggcagctggt cgtggaaaat cagaacggca tttatgaaaa gaacgccatg
    47641 ctgaacaacc ctcagtcatt tgacggcctt ggcaataatg cttatccggc cgccgctaca
    47701 gcccccggac gcacgttcag cttatccctc gtcatccgct atacttccat tgattaccct
    47761 tcttaacaac atatcaacca tcatcatgaa cgaaccacaa gaacacggaa tagaacggct
    47821 tttcgtcgaa ttcgccgaag aatgcaacaa gaacccgaat ctgaaacagg cggcgcagga
    47881 gatgcgtgaa ggcgtctttg aggctgcgga atccgtcggc gttaatccct cccatgcctt
    47941 cggcgtcatc atccgcgata tgatgatgct ggaattcctg cagaaacgtg tggaagaatc
    48001 ccggacggcg ctcacgtccg ggaatttgcc cgctttcgtc gtcgaagaag cgaaaagaga
    48061 attttaatca actaatagcg aacaatttac ctttccaaaa tcaggagtac caaaaaagaa
    48121 ataaccgttg aagttaaaag tacctttgac accaaaggaa ttcaggaagc aaagaagcag
    48181 attgagactc ttggcaggat ggaagccgga ggcgaccttt ccgcctatga caaggctctt
    48241 gaaaacgcca atgagaagct gggggaattc gccgataaga tgacggatgc cggctcccgc
    48301 atgcgggcag ctttccggga caaccccgga cttgaggcgt ttattgacga cgcgaccgga
    48361 gccttattga cagcggaaga acttaagaag aaatttgaac agattgatga cgtcgcggca
    48421 gtcctcaata acaaaatgtc cgattcggat ctgggggcga agtggggcga aaaccttgat
    48481 gaaaacctcc aaaagatcct cgacggctac aataaggaaa tggccgcggc cgacaaggcc
    48541 acggaaaaag cggctaccgc ggagacccgg aagcagcagg ccgcggccgc cacggtggaa
    48601 cggatggaag ccaacaaccg ccgcgccgcc gcttcctatg aggaattaga ggcggagctt
    48661 gcttcatata ttgccaagtt ggaagaagcc cggaaggctg ggaacaacgt ggcacaggcg
    48721 gatgccttgc ggaatatcca ggaattggaa aaaagactcc gatccgccag tgccggcagt
    48781 caaaaggcaa ccaggagtat caagaatatg gggcagggag ccttgcaggc tgcctacttt
    48841 tttgacgact tgcaatacgg catcaggggt atcatgaata atattccagg gcttgtgatg
    48901 ggcttcggag gcggggcagg gctggccggg gccatctcca ttgccgtgtt agccggcgcg
    48961 aagctttacg agtggatggg gaagacggaa gacaagtcgg cggatcttgc aaaaaaaatg
    49021 aaggagcaga gcaaagagat tgccgaatca tatcggaagt ctcttcgggc aagttatcag
    49081 gctttgcagg aattcaacca gcaagaacgc accaagaccg ttaatgaaga ttatcaaaac
    49141 tatgtaaagg gcattacccg tgaatttgaa tatcaaacgg aagaacttga aaaacaaatt
    49201 cgcctgagac gggaggaagc ggcccgtaag aaaggaattg atacacgtca ggcggagctt
    49261 gatcgcgtca atttggaggt agactaccag gaaggaagaa tctccaagag gcagcgcgat
    49321 tatggtctaa tgatggttga tcagaatctg gaccagaaaa tccgccagcg tgatttaagc
    49381 gtggagcaag ctaattatat ggatatgggg aaacagcttg cggaggtagt caaggccaga
    49441 gatgcggccg aagctcatgc ttttgatatg cagtttaaac aagggaacct tccttccctt
    49501 caaaatatca tggggctttt gcagcagcag gcaaacgctc aacaacgtat tgatagtctt
    49561 aatagcaatt tattaagaat acaggaatta cgagaaattg gaacttggcg ttctcccaca
    49621 gaacagaaag aatatgaaga tgcagtagtt agagagcaac aatttataaa agctcgtaat
    49681 tcagctcagg aagaagggaa tgccgcacaa ttaaaattga atgaatatca ggacttgtta
    49741 agagccggag gggtgaatct tgaattcgat tatggacaag gatcagatgt gaatagccga
    49801 ttcaaacaag caattactgc ggtagaagaa ttcgaaaaaa atacggatgc cgcgaaaaaa
    49861 cagtttgatg atttaactaa aaaggccggt tcattaggtg atgctatggc gtctgcggag
    49921 gcgtccataa aagacatgga acaaaccgaa gcccttcaaa atcaaataga cgccggcaag
    49981 gttaaatcgt ttaacttgca aagagacagg gaagaagcgg aggaagcccg aagaaatgaa
    50041 gaaaaaataa agaaggctcg tgaacaggcc gaaagagagg cgaaaaagca gacggccgag
    50101 aggcagcagg caatgatccg cggcattacc cttgaaggcg tgcccgaaca gcctaccgcc
    50161 aaacaacgcc cccggatttc tgccgcccgc gaagccttga acgaagggaa gaaacgcctt
    50221 tcggaaagca tttcggcaac agatacagaa atagatccaa gcgaacttct gggagcgttt
    50281 gacgctgtgg aaaaagtatt ggtagaaaga ggacaataca caaaaaaggc gtttgatcat
    50341 ttggtctcaa ttattagagc gcaggccgcc aaaataaatg cccaggaagt gaatactcaa
    50401 aaatatatag atgacaaaat agaaagacta ctgaaagagc agcaacaaac caacacaaga
    50461 ttgagaactg gaataaacag gcttgccgga ggctattaac agcttttagg cttgcactaa
    50521 gtatggttta tagcaccata tcggtatgaa taattattac gtcgcttccg actcaaacaa
    50581 tgctgatggg ccctactctt ttgaagtact tgaagccatg tacaaggaag ggataatctt
    50641 ctcagaaacg cttgtttgtc ctgaaggagg tcaagagtgg ctatcgtttg gaagggttta
    50701 tcatctgacc aaaaatacca acagaaaatc agatgaaaaa gaaacggaga gcaactcaaa
    50761 gaagagcttt gttgaaatta ttttagatga ttcagaaagg gcttcaaaaa cgaataaaga
    50821 gccaatagaa gacatcactg ttgaggggat attccgtgta gtcggggtaa tggctttact
    50881 tgctgggatt atcctccttt tagtttatat gtccgaaagc tttaaccaac cgtcattagg
    50941 tttcatctat ttgctatcag gagccttctc ctgcctcgtc tgcttctggt ttgcgaaggt
    51001 tctgacattg cttcaaaaga tcgccgacaa gaaataaata aaccactaac cattttacaa
    51061 gcagcaggaa ccacgacatc accatcaatg atttgctggc tctcaagccg tccagcctga
    51121 cccacgacca gcaaagtttt gccgcctcca ccattaccgc ggtttatccc gtccgcaccc
    51181 tggacgaggt cttgcccttc cagcaattcg acaccgtcac catctcccaa aacgggaaca
    51241 ccattcttgc cggcctcgtt tccagcatcg aaaagaccta cagcggttca tcccgtgcct
    51301 ggaaaatcgt cttttccgac ccctggtatt acctggacaa ctgctttgcc cttgattccg
    51361 aatggaaacc ggtcttttcc atgtggaata aagtcagcgg agggggcggc atcgtcccca
    51421 aaataagcat ttcctccgcg ctctcccggg tgctgaacct ggccaaacac caccccgcgg
    51481 actacgaact gcggatcagc agcgacaaca tgctgatccc gtggaacgcc tcatgcgata
    51541 cgctgggaag ccttcttcaa tccatccgcc gctggtcgcc ccgcatggcc acgtactacg
    51601 actacagcgg cgcgcgtccc aagctggtca tcacggacta tgacgccctg acgcctatcg
    51661 acctgccatt gcagccgacg acgacggtta agtccatgga tgtatccctg gtcccccggg
    51721 ccgacctcgt gcctccctgc gtggcgattg tcgcggaaac caccggaagc aacggctatc
    51781 gcgtctctta cctgtccaaa tacccggaag acggagaccc gaccttgccg cattccatcg
    51841 tttaccgaac gtcggtggac ttctactact ccaaatacag ttctaccggt tctcccgtag
    51901 aagttcctca accggtacag ggaaccaggg ccggcagcct gtcctaccag cgcatgaaag
    51961 tgacggggac ccggattgac cagaacgaca tgatcaacag tttctggaaa aatcatttcc
    52021 cctggatgaa agacgtggac gccctcgcgg cctatgataa ggagcctgtc atcaccggga
    52081 agccctggga agggacggag gaagacaaac cgaaaggcta taataccacg gccacgggct
    52141 atgaactgac ggacggccaa attcatacca aatccatccg gccgcagtgg tgcaatgcca
    52201 ccatcaagca gcgtctcgcc atcccggaaa gcgcccctgc caagtggcgg gagaaattca
    52261 agaatgtggg aacgctggga ggcaagcctt acttctggga agaattctcg gtagatctgg
    52321 tcaccatgga ccgccccttt gcgagctacc ccatcgacgg catctacaac ggggagcagc
    52381 cctccaacgg gccggaagaa ggggaatcgc cggaaccgtc ggaaggtgtg ccctatgggg
    52441 acattgccaa aacggtgtgg gaatccatgc aggaattgcc ctgggacggc tctatttcct
    52501 ttgtcgcgct gggagaggct caaacgcggc aatacatggg ccgccgcgtc tcgctgctgg
    52561 gagggaaccc agcatggcag aacatcaaca ccatgattca gacggtgaac cgcgacctgc
    52621 agaccaacgt cattacgttg tcctacggcg cgccggaaca gctgggaatt gaagaatggg
    52681 tagaattgaa gcgcgtgaat gccacatcac gcagatcctc cacgctggaa aacatgcagg
    52741 gcgcgccgga atcgacggaa tacgcctttg accccaagcc ggaatctccg accatcagcc
    52801 agcatattac caagtccacc ggagaagcca cgcccgcgcc ggaatacggc tttcaggtgc
    52861 gggtacagaa agatacggag ggcgccgtca ccggggcgca aatcaagccc ggcgcgctct
    52921 acctgaacgg aaatctgctc ggcaaatacc cgcagggggg cagttccggg tcctcgtggg
    52981 tgcagctcac ccaaaccagc ggggaagtat ggctcaacgt ccactttgac caggacgcca
    53041 aattgacggg cgttgacgtc tccggcattc cggggacggt gtacccaatc aggcttgccg
    53101 aagaaaaacc gggcgtcaac ttcgactatt ccttcctgat tgccgacatc gaagacgacc
    53161 aggtgacgca atacgccatg ggaatgattc agataccggt cttcggagga accttctacc
    53221 cctatggtcc tgcttaacca gacacatcat gatcagaatt tacctattca cctatgccgg
    53281 cgacgccgat gaagctctcg tctgcgtccg gtgcgctgcg gcggccctcc ccgaagccgt
    53341 catcacggtg gtggacgatg aatccactcc cattgctgaa cgcgccagaa ctgcccttgt
    53401 tgaagccggg gcgcggtatt gtcaaaccag ctggccaagg agcgggaacc tacgcgggcc
    53461 ggaatgcgtc cggggcatca tttccacgct ggccggggaa gcggaggacg aagacatcat
    53521 cgtcaaaatc gactccgata cgctcctgtt gtcgggggac tgggtccgag acatgcagct
    53581 ccatgggctg gcgctccatg cctccggcta ccaggtcccc agccaccctt ccgaacgttc
    53641 cgcctatggg ccgtgttacg ccatcagcgg ccgggcggcc agactggcgg cgaaggagct
    53701 ggaacaggct gaccttcctc cgctcgcacc ggaagacctc accatctgcc gggcggttca
    53761 gaatcacttc cccacggaac aaatccgcct tgatgaaccc tggacgccct tttaccggga
    53821 agggaagtgg acggcctgga actggttcag catcgccgtg actccgcaga aatacgcgga
    53881 tttctggatg gttaccttcg gcaacccgcg cccaacgaac atcccgaaat acgagcgggc
    53941 ccgcgcgatg gacgccttgt tccgctaccg tttcccgcaa aacgaagacg gatcatcttg
    54001 ttgacgtcac caacatgatc ttaatcttgt aatgtaggaa agaaactgta caaaatactt
    54061 aacattattc gtataagtat attataatca tgaaaaagaa tccctcccgc tccgccaaaa
    54121 cctttctttt ttaagagtct gcactttaac ggtgtaggct ctttttcttt gcaaaccagg
    54181 ggattagatg ctggggaatt atttgctggt ctgattgtct tattttagtt ttttattttt
    54241 tcatttgaaa ttgaataact taggccaaaa acgccggtta ggatgcatag ccagcgtttt
    54301 ttctaatatc cttattatag gcatcaggat aattataggt gcatttggaa aaaggtctgg
    54361 actggaggct gtccctgagg gtatttgtgt gtgtgaatga tgatataaat ttttgaatgt
    54421 aagttagttg ttgcaggaga tggacaggat aaaactgccg tggctggtaa aagtggagag
    54481 tgcccggaat tgaatggaca cgaatgtggt tcatgcgctg gtctcggagt ggttcacggc
    54541 cgcatcggat atgttaacag cttcaggatg tgtgggaaag tatggaacag ccactccaga
    54601 atgggctctg tcttttttta atctgcggag gatttaacgg attttggaat aatctggtgt
    54661 tatcaatggt caagaccatg aataacaata ttaaagaaca tcctatccaa tttgaagcag
    54721 atatgattcg ggcattgctg caggaataca gcactcccgg cgagcacaaa aatcaaaccc
    54781 gccgcacgtg cggcctgaac cagtttaatg atctccctga agctttgaaa gaaagagggt
    54841 gggagattca ggaatttata gaagagtccg gctcgtggct ggctgtttcc gaggaaggaa
    54901 gcggaaagtt ttcagatatt tttatcccgt ggattgaatg tccctacggg aaagttggcg
    54961 accgtttgtg ggtgcaggag gagatttgcc ataatagccg ttctggatgg ctttattctg
    55021 ctgatggcgt ttgtttgcca atgtcatatc cagacggatg gatatcaaaa aatgcccgca
    55081 ggcagtttat tccttcgatc cacatgccgc gttgggcttc ccggatttta ctggaaatta
    55141 cggaaatcag agttgagcgg cttttggaca ttacgacgaa cgatgcacgg atggagggca
    55201 ttgaatgcgt ttggcacgat gaagaaacgg atgcctgttt atggagggat tactcgggaa
    55261 aatctgcggg gttggctttt gcccggttat ctttcttctc tttgtgggaa aaaataaagg
    55321 gaaccggttc cgctgaactg aatccgtggg tatgggttat taagttcaat gtgttaacta
    55381 tcaacggaaa tatcaaataa agaatgttaa tgccctttgt gaagaacaaa ataagaatat
    55441 gtcttttaca gagggttcgc ccactaagcg gagggaatag cctccgttgc aattcagata
    55501 aaatccgcgt gatgtaatca actccgtttt atcatgaaaa aagcgccttc cggcaaggga
    55561 aggcgctgga tatgaaaagg tgaaaaagag ggaggttaca tccccttgtt tttcatggct
    55621 tccgccaggg cggctcccat ctgagccggt gtcttggcaa ccacaatacc ggcgttcttc
    55681 aaggcttcct gcttagcggc ggcagtacct ttgcctccgg caacgatggc tcctgcatgg
    55741 cccatgcggc ggcccttggg cgccgtggct cctgcgatga acgcggcaac aggttttttg
    55801 cagttgttct tgatccattc agcagcttct tcttcttcag aaccgccgat ttcgccaatc
    55861 atgatgaagg cgtccgtgtt cggatcttcc gtgaagaatt gcacggcctg ttgctgggtc
    55921 atgccatgga cagggtcgcc gccgatgccg atgcacatgc tctggccaag gcccatgttg
    55981 gtaacctgcc agacggcttc ataggtaagc gttccggaac gggagacaat accgattctg
    56041 ccgggtttga agatgtaggc cggcatgatg ccgatcttgc agttggcgga aggattgacg
    56101 attccggggc agttcgggcc gatgagggtt acgtccttgt ccttcaggaa ggttttcacc
    56161 ttctgcatgt cctgcacagg aatgccttcc gtgatgcaga cgatgagttt aacgcccgcg
    56221 tcagcagctt ccatgatggc gtccgctgcg aagggcggag gaacaaaaat catggaggca
    56281 ttgcagtccg tagctttttt ggcttccgcc acagtatcga agacggggac tttgccttca
    56341 aagagctggc cgcccttgcc gggggtcaca ccggcaacca cattggtgcc gaagtccatg
    56401 cagtttttgg cgtggaacgc gccggcgctt ccggtgatgc cttgcacgac cagacgggtg
    56461 tttttgtcaa tgagaggtgt cattgtcgta ataaatagtg agggttattt aactgccgca
    56521 accgctttct gggcagcttc gtccaggttg tcggcaggga taatggagat gccgctgtct
    56581 gcaaggattt tcttgccgat ttctacgttg gtgccttcca gacgtacgac gagcgggatt
    56641 ttcatatcga tgtttttcgc cgcagccaca atgccctggg caatgacatc gcaacgcatg
    56701 atgccgccga agatgttgac cagaagggcc ttcacgtttt tgtcgctggt gaggatgcgg
    56761 aatgcgttgg ttaccatttc ctccgtggcg gagcctccta cgtccaggaa gtttgcgggt
    56821 tcgccgccgt agtatttgat gatgtccatc gtagccatgg ccagtccggc gccgttcacc
    56881 atgcagccga tgttgccgtc caggccgatg tagttcaggt cgtacttgcc ggcttccact
    56941 tcgcgggggt cttcttccgt ttcatccctc atttccatga tttccgggtg gcggtacagg
    57001 gcgttgtcgt caaaattgaa tttggcgtct agagcgcaca cgcggtcatc cgtggtaact
    57061 acaagggggt tgatttccac cagagagcag tccagagcgg tgaaaagctt gtaaacgttg
    57121 gtgatgagct tggtggtctg gcggagcagg ggaccggtca gtcccagcgc cacggcgatt
    57181 ttgagagcct ggaaaggcag gatgcccagc gccgggtcaa tgtgttcgcg gataatggct
    57241 tccgggcggg tggcagccac ttcttcaatg tccatgccgc cttccgtact ggccacgata
    57301 nnnnnnnnnn gcattcccgg gcgcggtcct gaagaatggc gaagtagcat tcctttttga
    57361 gatccacggc ttccgccacc atgattttgc tgaccagctt gccggtttcc ccggtttgct
    57421 tggtgaccag aacctggtta agcatcttgc cggcgacttc ttccacttcc tccacggact
    57481 tgacgacgtg gacgcctccc ttaaaaccgt ttttgaaggt gcctttacca cggccgccag
    57541 cgtgtacctg tgccttaact acgtattggg agaggcccat gttccgggct gtttgtgctg
    57601 cttcctgagc tgtggcggcg gcaatgccct tgggggtagc cacgccaaaa cgctcaaaga
    57661 gttgttttgc ttgatattcg tgaatgttca tgacagtggg ccgtttggcc aaatgtttgc
    57721 ggaagaaagg ctaggactat ttttgatgtc ggtcaaggga gaatggtttg gtcttttgat
    57781 tttttgtccg gaggtgccgt gaagccctgt tggaaatcaa agaggacaga aggcttgact
    57841 gcgtgcttaa agattgggaa acttccttcc cgtttttctg tagagacttt ttatgaaaat
    57901 ttaccgtcct tccgatacct gctttgacga aatgaagcgc cggatgaacc gccgcgccct
    57961 cccggaggat tccgtannnn nnnnnngatg tttccgcgcg cggggatgaa gccctttttg
    58021 acattgcctc cagatttgac aaggtacatc ttgattcttc ctccctgttc gtgacggagg
    58081 cagagctggc ggaagcggag gccatggtgg aggggagtgt gaaggaggcc attgccgtct
    58141 ctctggcaaa tatccattat ttttcagacc gcagccgcag gcaggactgg tccggcgtaa
    58201 atgcgcaggg cgtggaggtg gcggagcggt tccttccgta cgaccgtgtg ggcatttata
    58261 ttcccggagg gaaagcgccc ctggtatcca cttccatcat gacgggcggt tttgctcagg
    58321 ccgccggcgt gcgggagatt gtggccgnnn nnnnnnngga ggccaaacgc cagttggtgg
    58381 gggctgttgc cattgatttg ctgcccggac ccagtgaagt aatggtgctg gcggatgata
    58441 ctgcggatgc agagtttctg gctgccgacc tgctggcgca gggcgagcat ggcccggaca
    58501 gcgtagttgt ttttgtcact acatcagagg cgttattgga gcaggtggat gcggaagtgg
    58561 aacgccaggc cgccctgctg agccgcggat ccatcatccg ggaggtgctg gacaagcatg
    58621 cctacggttt tctggtttct tccattcagg aaggggtgga actggttaat gcctttgcgc
    58681 cggagcattt ggtgcttgtc actcgggatg aagaggccat gcttaatggc atcaggacgg
    58741 cgggcgccat ttacgcaggc tccctttcta ctgtagcctg cggggatttt ctggcgggtc
    58801 ccagccatac gctgcctacc ggcggcgccg gcaagtcttt ttccggcttg cgggcggacc
    58861 agttccagcg ccgcaccagc gtggtgcgca tgaaccggga tgccgtgctg aactccgccc
    58921 cgtatgtggc ggagtttgcc cgggtggagg ggctggacgc ccacaaccac tccattcagg
    58981 ttcgcgccgc ccgcgtgaac cggtaatccc ggaattttcc tcaatgctct ccatgatgca
    59041 acagcaggga atcagggaac ggttgcccgt tgcagaagat ctccttgttg ccggacatgg
    59101 ctatatgggc gtttcccggc atcatgggag tggagtgggg gccgttgctg agagcatggt
    59161 agtcggcttg ccgacccgtt tgcgagattt gtcgggatgg aacgctcatc ggatgggcgg
    59221 tttcatccat ttgctcggaa atcccgcgta atcgtttcac cgtattcctc atgttcaagt
    59281 ccctcatccg cacgtttgcc attgtggcgg ccttcatcgc ggggtacatg atgcccggcc
    59341 tgcatgtgta cagctggaca ttcaagtgga tgctggtagt aatgctgttt gtaacctttc
    59401 tgggcatccg cttcaaacga atgaagccgg aacgggctca ctggctcctg acgggggcta
    59461 atctgatggt cgccctggcg gcatggggtg tgtgccgcct tgtttttggg gatgggtatc
    59521 tggcacaggc cgcttttttc gtaggcatca tgcctacagc cacggcggct cccgtggtga
    59581 tggggctgct gggaggcagc gtggagttta tgctgaccgc ccttttgcta attaacggta
    59641 ttatctgcgc cttgctgcct ttcatcctgc cggcggttgt tgggcggggc ggttttgaga
    59701 tttatctgaa cgtggcccag aatatttcct tggtaatgtt acttcccctg gctctggcat
    59761 tggcggcgcg gcgcttttat cctcgcgcca tcgcgtggcc caggaagttg aaagacgtca
    59821 cttttggcat ttgggtggtg attctggttt tgatagccgc aaacgcctct tatgatattt
    59881 cctcccgtga gggcatttcg gaacgtgttt tggaacagat tggcgtgatt gccctgctgg
    59941 tttgcggggt caatttcggg ttgggatatt tgctgggggg acgcacgcgc gctgcggagt
    60001 gcagccaggc attagggcag aagaatacca ccctgtccat ttatctggct ttgacgtatg
    60061 ccagccccat tgccgctctg ggccccacgt tttatgtact gtggcataat ttatggaatg
    60121 cctggcagtt gtaccgcgcg tccgaacgaa agcgcaggga tggatgagga agggggcatc
    60181 cggtgcggcg ggagtccccg gcagaattat ggggatttcc atggtcggaa aaacgggagg
    60241 gagggttctt ttgagccggt tcccagtgac ggccggcaga ctgttgaagg ttctttttta
    60301 tccactttca ggaacggcag aggagttctc cccgagcaaa aagcttgaaa ttcggggaac
    60361 gcccggtaac gttatgcact tgttatgcag gcgtcttctc ctttgccccc tctgtctccg
    60421 cgtaccaagt ggctgattgc cctgtgtttg tgcgtgcttg tgatgctgct ggagttcctg
    60481 acgtaccaca tggcgtaccg catcggctat gatgaaggga tgctgactac tccgccggtg
    60541 gtggtgcaga aaagcgatga aaaggccatg cagaatttgt cccgcttcat ggcggatgtt
    60601 tttgcttcgc gcgagagcct ggccggcatt ctggacaacc gggaggaacg ccttgcatgg
    60661 atcagggatc cggaactgcg gacagaaacg gcctggggcc tcacccggga gctgattagc
    60721 cggggtggcg tcgttcctgc tcttcctgcg gccagggaat taattgacgc cggatacgcc
    60781 gccgggcgct atcaggtatg ggctccccgt gcggatgccg tggcaaaggc tctgttggcg
    60841 gagcaccagt acacttccgc ctacgattat ttgaaaaccg ctgaggaggg atatgagaag
    60901 gaaggaatgg ctgagccgct ggtgcggacg ctccagacca tgagttccat tgaccagatg
    60961 ctgaacagga atgaacaggc taatatgttg ctccagcgtg cggtggatgt cgccgcacat
    61021 ctggggccgg atgccctccc tgtcaggtcc aggcttttag ccgcccaggg aaggctggca
    61081 cgtactacgg ggcgcattcc ggaatcgcgc gagtatttca agaaggctct ggctctttgt
    61141 cctcagccgg acaagaccac tggagacttg gccctggcca gcatcagcat gggggaggcc
    61201 atgttggaag ccggcaggaa ggatgaggca cgggaattgt ttttaaaggg cctttccggc
    61261 tcggaaaatg cctcttacct gttgaatgac tgcctcaatg ccctgcgcgg tctggcccgt
    61321 atttctacgg agcaggggaa ttatgaggag gccctggctt atctttacca ggcggaaggc
    61381 gctgcccgcg gcgtactgcc ggcggaccat gccttttggg ccgggttgta tgatcagaga
    61441 gggtgggtca acatcttgcg caaggcctct ccggaagccc gggcggattt tttaaaggcc
    61501 attgccaaca gctcggcttc tcccgtcatt tccgcccagt cccgcgaagg gctgggcaag
    61561 gcgtggctgg atgccgggga ggggggcaag gccagggaga acctggaaaa ggctcttcag
    61621 ttgcggaagt cccatttcgc ctcggatctt ctgtccctgg gccgcgtata ttattccctg
    61681 ggacttgcca gcgatatggc gggggacagg gagggcgccc tgactgccta tgccggagcg
    61741 gtggattctc tgctgaaatg cgtggagggg gcggaacgca gggatttgct ggttcagtct
    61801 tatttgtgta aggcgtacgc tctgtgtgac ggagaacaat ggaaagaggc ggcagaagct
    61861 tttgaggcgg ttcttcccat gctggagggg gaacagaggt cggagaatta caaacagctt
    61921 ggacgctgtt atgacgaact gggcatgaag gagaaagcgg atgactgctg gaaggaatcc
    61981 ggttttcccc gggtgcgcat ggcatccccc gggcgcagac cctccgtgaa ctccagatct
    62041 tcacggaggt aggatgcgag ggacttcgta agacgttgcg aggcgtttat tttgcttgat
    62101 ttgtgccgtc tttttggctc aatgcctgca tgagttccga gcgccatttt tccagtaagc
    62161 cctaccgccc cgcacgcaag aaaaagtctt attggaagcc ggtcctcttt ttggtcctcc
    62221 tgggaggact ctggttcggc tacatccagt actggcctac gatagaaggg tggttcaagc
    62281 cgaccattca tgaggtgcac agccagacgc aggccatggg gcatttgcag gatatgctgg
    62341 cttcatggaa cggttccgat gcagagctgg cgcagatggc atcctccgtg gacaagcagg
    62401 tggaatggat gaattctccc gaagccagag attcctttgc atggcttttg gcggtggaaa
    62461 tggaccggcg cggcatgctg aaagaatccg aacctatgtt ggctgctctt ctgggaaaaa
    62521 agctggctgc ttcctcctcc atgacggcgg aggacaatac ccgccttctg ggcgtcagcc
    62581 tgaactgggc gcgggaattc gcccgccgca agcatgatgc tgtggcggaa aagctttatg
    62641 aagtgatttt gaaaaatacg ccggaagacc aggtttccat ccgtttagcg tgcttggagc
    62701 cattaatcca ttatgcgtac gaccaggcca ggtttgagcg tttttccgcg ttgtgcaagc
    62761 aggctatttc tcctgtcatg aggagcatgc tgaataaacc ggaggatgtg aagagcatgg
    62821 tgaaaatcct gttgcttcag gatactctgc cagacaggac caccgggttg cccggtggca
    62881 cggggagtgc gattgcccgg gagcttctta ctaaattccg cctgaccgcc aatccggaca
    62941 tgggccgcat tattctgaat gaactgaaga tgccgctgaa cacgcgccgg aagtattccc
    63001 aggaggaatt gaaggccatg gccgaccagc tggaagcggc cctgatctgc ttccgtgcag
    63061 ctgaaacgga gatggactgc acgccggaga caatgcttgc cctggcacgg gtaaggatgc
    63121 agatggggga tttgagagaa gcctcccgcc tgctgtcccg tgcagaaggg gctgccatga
    63181 cgcttggcgt ggatgctccc cgtattttga gcggctccag cctgagccag gatattgcgt
    63241 ccatgcggtt ccagcttgaa aaatacagcc aggcagaggc tttggtgaaa cgggcttatg
    63301 aagatgtgaa tgttgccgca gctttcctga aggcgcggga ttacgatcag gcggtaaagt
    63361 atctggaaca ggcaatgaaa gtggctcgtg aaaataccac gtttgttcag gcacttcagc
    63421 cggtcattct gaatcttcag gcggagataa gcgccggcag ggaacaatgg gctctgtcgg
    63481 agtcccaata tggaaagtta attgcggagt gggatgcgtt gactccggaa gataaggaaa
    63541 aactccggaa taatctggcc tccatccagt tgggagagct ttacaatgaa attcaccgca
    63601 actgggctgg tgtgtgcctc aagcaaaaac gaaccacaga ggcccgcagg gtgctgggga
    63661 agataggaga ggttccggct gaagaaccgg agaggcctgc ctcgcgccga cgccgcagat
    63721 agcagaccct tccttcagtt aggccgttcc ttttttggaa aggaaaagtt atccattggc
    63781 agatgccgga ggggctcaga gttcccagag gctccggtcg ggagcttgac taaagcggga
//
LOCUS       DAWEMX010000003        52456 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_293_length_52456_cov_12.475163, whole genome shotgun sequence.
ACCESSION   DAWEMX010000003 DAWEMX010000000
VERSION     DAWEMX010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 52456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 52456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..52456
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_52456_cov_12.475163"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1199..1208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    38783..38801
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    39454..39463
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    45914..45923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    50358..50367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11981 a        14212 c        14277 g        11927 t
ORIGIN      
        1 ttgatatgga aatcataaaa aataataaaa tcatcgttcg caaggaagac gcatcgaaga
       61 aagtgttcaa gggcgtttca ctggattcgc tcgctgtcgg agagaaatca atggtagcaa
      121 agatgaacta tgtgaaaggc aacttcgcct ccacccacca acatccgcat gaacaatgcg
      181 ggtacgtcat ttcaggcgaa taccgcctga acatggaaat gccggaggag aatattgaag
      241 tgctgcttca tgcaggggac agctacgcca taccgggcaa tacgcctcat tcctttgagg
      301 tgatggaaag cggagaggtt gtcgatgtgt tcacaccgcc aagggaggac tatttgtaaa
      361 cagccgcatt tggggctgaa gaaacttcat atcagcctaa taaaactaac gagaggatgt
      421 cgccccccta ttgctgcatt ctgaaaggat tatccggcag cataccgaac gccgaccaat
      481 ttgttgtctc tgtttttcaa cagattaggc caaactgaca aaaatccaat aagaaaaccg
      541 ccccggtaac cggagaaaga cgcggaacca aggcaccggc tgatgctgga cgatttttcc
      601 gccaggatat tttccggacc ggggcggtca attacatccc gctcatcggg aacaatgtat
      661 ccagcgcggc gttcagagtc atcacgttga ctacgttttc ccccagaatt ccaccccgga
      721 cgaagtgtgc agacaccagt ttccggactt tttccggagg tactccgcgc gtggaggcaa
      781 cacggttcaa ctgcaactcc gccgcttccg gagagatgaa aggctccaaa ccgctgccgg
      841 aagcaaaaag catgtccgca gggacaggcg ttttctccgg caggttgtgg gctttcagca
      901 gagccgccct gcgctccgcc accgttctga ccagcttccc ggaagtccag gagaggttgc
      961 tggctccgga aggcatggta gcgtattctc cggcagaggg ccggggccag aaatatttgg
     1021 cggagataaa aggctgtgcg acaagctccg aagcgataat ttccccccgt tcattccgga
     1081 ttagggaacc atgcgcctga tgaggaaaaa aaatcaggct gaccagcgta acggccaaag
     1141 ggtaaatgcc gccggtaatg acggccagaa acagaaacag gcgtagattg ggaaatgtnn
     1201 nnnnnnnngt tcaaacaagg cgccatgcat tgatgaccat atcaataagc ttgatgccca
     1261 tgaaaggagc aatcaggccg cccaccccgt aaatgaggat attgcgcttc agcagggagg
     1321 ccgctcccgc tggacggtag gaaaccccct tcaaggccag cggaatcagc gctacgatga
     1381 tcagggcatt aaaaatcaca gtgctgagaa tggcgctttg cggggaattc aggcccatta
     1441 tgttcagggc ggaaagaggt ccactggcca tgccgtcagc cacaaagagg ccggcaaaca
     1501 tggcgggaat gatggcaaag tatttggcta cgtcattggc gaaggaaaag gtggtcagcg
     1561 cgccgcgggt aatcagcatt tgcttgccga tttctacgat ttcaatcagc ttggtcggat
     1621 tgctgtccag gtccaccatg ttgcccgcct cccgggcggc ctgcgttcca gtgttcatcg
     1681 ccacacccac atcggcctgg gccagggcgg gagcgtcatt agttccatcc ccggtcatgg
     1741 ccaccaggcg gccggcagcc tgttcctcac gaatgcgcgc cagcttcatc tcaggcgtag
     1801 cttctgccat gaagtcatcc acgccggctt ccgccgcaat ggatgcggca gtcaaggcat
     1861 tgtcccctgt caccatgaca gtccggatgc ccatggcccg gagccgggca aagcgttccc
     1921 taatgccgcc cttgacgatg tctttcagat aaatgacccc cagcacgtct tttccccggg
     1981 ccactaccag cggcgtaccg ccgacacgcg cgacattctg caccatcatt tccacctctt
     2041 gcgggaaagt tccgccccgg attttcaccc actgcgccac gctttccgcc gctcctttcc
     2101 ggatggaaac gaccggacgg ccgtcgtgct ccgcaatatc cacgccactc attcgggtgg
     2161 aagcggaaaa cgggataaaa acaatatggt ccgcgtccat atggcggccc cggattccaa
     2221 actgtttttt agccagcacc acgatgctgc ggccttccgg cgtttcatcg gacagggaag
     2281 caagctgggc agcttccgcc agctgaaggt catccacccc gggtgcaggc aggaattcgt
     2341 tcgccattcg attgccgaag gtgatggttc cggttttatc cagcatcagc acatcaatgt
     2401 ccccggctac ttccacggcg cgcccggaca cagccagcac attccggcgc acaagccggt
     2461 caatgccgct gataccaacg gcacttaaca atccgccgat cgtcgtcgga atcaggcatg
     2521 ccagaagaga gaccaggatg ggcagggaaa tacggccctc cgccctgaag ccaagcgccc
     2581 gggcagaatc ctcaatgtat ccggccatgg cgggaaggga aagaaccacg gcaagaaaaa
     2641 caatggtcag cgccaccagc aagataccca gagcggtttc attgggcgtt ttctgccggc
     2701 tggccccctc caccatggaa atcatgcggt ccaggaacgt atggcccgat tccatggtaa
     2761 tacggataac aatgccgtca ctgattacac gggttcctcc cgttacagcg ctgcggtccc
     2821 ccccgctttc acggatgacc ggagcggatt ccccggtaat ggcggattca tccacgctag
     2881 cgatgccttc tatcacctct ccatccaggg ggatgatatc cccggcctcg cacaacactt
     2941 tgtccccttt gcgcaattcg gaaacggaga cgatttcctc cgttcccttg tccgtcagca
     3001 aacgggcgtt cacataaacg cgcatttctt tcagggcgtc cgcatacgcc tttccccggc
     3061 cttccgccag cgcttccgca aagttggcga agagcacggt gaaccagagc cagagcgcga
     3121 tttgcaaagt aaagccgaag ggttgcccgg cagccaaatc cctgataagg gcaatcgttg
     3181 ccatcagggc gccggcagcc gtgataaaca tcacgaagtt tttagacagg acgcgcgggt
     3241 caaatttgcg cagcgcattc aggaaagctg ttttcaacat ggctccgtca taccaggatt
     3301 gactcttttt ctccttcatg atcataaaca tccggattat cggctaatac gccttaaagc
     3361 atggctcccg aatacaggag taaatgctcc agaataggtc ccaaagccag ggccgggaag
     3421 aaggtgaggg ctcctacaac cagcacgacg gctaccagca gaaccatgaa catcaggtta
     3481 tccgtcccca tggtgccctg ggccggcgga gcggttttcc tggatgccat gctgccagca
     3541 attatcatca ccgaaataat ggttccgaag cgggccagca gcatcgccaa cccgcccagc
     3601 agggaataaa aaggcgtatc ccctgcagcc agaccagcaa aggcgcttcc attattcccg
     3661 gcctgggaac cgaagcagta caggatttcc gtcagcccgt gggggccggc attgcagatg
     3721 gatgcccgcc cggcttccgt agcggcggcc aggccgctca tgaccaggac ggccatgccg
     3781 ggcagaagga tgcccaccat ggagcagcac acttcccggg ctcccatttt tttacccagg
     3841 aattccgggg ttcgccccac cattaaaccg cacaggaaca cggtaatcat ggcaaacatc
     3901 agcatgctgt acaagccgca gcccagacct ccgaaaatta ccccacccag cagcatattg
     3961 aacagggcaa tcccgccgcc cagcggggac atgctggaat gcatgcagtt cacggacccg
     4021 ttggaagatg ccgtcgtagc gacggaccag agggaactgt tcgtaacgcc caggcgcact
     4081 tccttgcctt ccagcatttc catgcccggg aaaagcgggt ttcccgcatg ttccgcccac
     4141 tgggacaaga caatggccgc cacaagcaga atcatcatgg ctccgaagat aatccacccc
     4201 tgcctctttc ttccgatcat gacgccatag gcgtatgggc aggcgcagcc ggccagaagc
     4261 agggagagca tttcaaccat attgctgaag ggcgtggggt tttcaaaagg atgcgtgctg
     4321 ttgtttccga aaaagccgcc gccgtttgct ccgagctgtt tgatggctat ctgggatgcc
     4381 gccgggccac tgggtatcat ctgttccacg ccatccatgc ccggcacggc aatgggtccg
     4441 tcaaaggact gcaccacacc ctgggagacc agcagaagag caatgaccgt ggaaacaggg
     4501 accagaaaat acaacgtgct gcgcgtcaga tccgtccaga agttccccag agtagaagcg
     4561 catctccttt ccagcccgcg gatcagggcg gacatgacgg caattcccgt accggcgctg
     4621 acgaaatttt gcacgcccag cccggtcatt tgaacaaaat aactgacgcc ttccggccct
     4681 tcccctgaat agaactgcca gttagtgctg gtcacgaagc tgatggctgt attcaaggct
     4741 atatcccacc gcatattttc agttccggcg ggattcagag gcagccattt ctggcacagc
     4801 agggaaagga aaaggacgag gaatccagcg gcgttaaaca aaagaacggc cgtcaaatac
     4861 ttcttccagt ccatttcgcg ggtggggtcc actccggcca tacggtagac gcagcgctcc
     4921 acgggaccca gaaccgggga aagccatgtc tgccttccca tcaaaacact tgccagccat
     4981 tttccgagaa acggggtgaa agagaccagt atgcctatga acaggacaat gattaacaag
     5041 tcgtgcgagg acacaacgca actctacgca tattctggaa attccgggta gttaacaaag
     5101 ggcctccgga cattaagaaa ttattaattc ctctccggaa gcccgttttt taacgccggt
     5161 gcgccgttaa aactccatgc ccaggcggaa tcccacccag aattcgtctc cggcatccct
     5221 gtgacgcagc accgtagccg cctgggaata attgacggag ccggaaagcg tataatgttc
     5281 tgcaaaccgc catgtcacgc ccaggctcca gtcaatgcag ttggagccgt catcatagtc
     5341 cacgttataa ccgccgttgt acccgtacct gaccatggga accacggtca cattttccag
     5401 gggggaccac tccaaccgca caaaggcgct gtaataggct tcaaaatcct ggtgttccac
     5461 tttcacctgg gtttccgctc ctacagccat ccaatccgtt acggcatagg agaccgtcaa
     5521 cgccggattg gccacattat aatcctgcgt aaaatagaat tggtgctcat accagggatt
     5581 gacagtccac ttccccagat tccaagcgta acccagaacc aggtccagtt catcgtaatt
     5641 ggcggaatct cctccggcat accaagcgct gaacgtaaga ttcttccaag aaatgctcgg
     5701 agccacttcc cagatgccgc tgcccggcag gcagtccaga ccttccgtta cgtacttact
     5761 ggaccacccc gccgagaagg aaaaggccca atccgtgtct gtggaaggta tggcggcctc
     5821 ctgcatgttt gtcagggagg ctatcgacgg agaaaccgtt tcttctcctg cgcagacggt
     5881 tcctgctatc atggcgcagc ccagggctgc ggctcctacg tttcttgcgt tgtacttgat
     5941 cttcataaag acgtattgaa tacgtcttgg atcctacaca gcatgttgga ctgcgtgaag
     6001 ttaagccaca ttctccaacc gttatgaaaa aatatatttt ccggctgccg cagataatgt
     6061 gccgctattt tttccagccc agtaaaactg ggacaagggc tactcggctc catccgccac
     6121 aattctgtaa cccaggccgg attccgcgcg gataatgcgg ttttccggat cggaatcccc
     6181 cagcttttgg cgcaggtggg caatgtgcac ccgcaggtag tgcgtgtcct cctcatgctg
     6241 gggaccccaa atttcacgga gcagctggcg gtgggtcatc accttgccct gatggagcag
     6301 aagcaggcgc agaattccgt actctttgga ggttaaatga atttcctctt tccccttctc
     6361 cacgcgccgg gaactcagat ccacgacaat gggacccagc cggaatacgg acggctcacg
     6421 gtccggacga ttgcgccgaa gcatcacgcg caggcgcgcc agcaattcac ggccgctgaa
     6481 cggcttggtc aagtaatcat ccgccccggc atccaaggca tccacctttt catcctccct
     6541 gtcccaagcc gttagaatca ggataggaac ctgagtccac tcccgaagct gtttcagcac
     6601 ttccaagccg ttaatgtccg gaagccccag atccagaata atggcatccg gccgcttcag
     6661 agcggtctca ctcaagccaa gctgcccgtt ttcacactcg cggacatggt agcctgcgcc
     6721 ggtcagcgtc aggttcagaa gacggcgtat ctgccgttca tcgtcaataa tcaggatatc
     6781 tgcaggaaag ttgctcatga ggcaggagga attactcttc aggaacacta tcgtgttcca
     6841 ccaggggaag gacaagccgg aaacatgttc cggcggaaac gggaaccagc gagacggcac
     6901 cgcgctgagc ttccatgaac ccgcgcacaa tggggagccc caggcccaaa ccaccgggcc
     6961 gcgtcgtccg gaaacgctca aaaatggttt ccgcccgttc gggaggaatg cccggcccca
     7021 tgtcatgaat atccagccaa acctggccgc gcgcggagtc ggtaccacct tgcaacgtaa
     7081 cagaggttcc ctcaggagta tgtacgcagg cattcaacag cagattgacc agtacctgtt
     7141 ccatcaggga aaaatccgcc ttcaccagcg gatagtccgg cggcagggaa acagacacgg
     7201 gatgattccg gcgtgcttcc ctggtagcag ccagagcccc ttcaataaca tcccccagat
     7261 cgcaccagtc cagtttgggt ttcaaagcgc cggattccag gcggctcaca tccagcaaat
     7321 tcttcaccag gcggcgcaga cggcgcgcgg ccagtaaaat ttccccgtat tcttcccgct
     7381 cctccggtgc ggacgcggca cgtccggcca atttttcaca gccgccctca atcacggcca
     7441 aaggcgtttt gaactcatgg gaaacactgt ccaacaggga tcggtgcagt ttttccgatt
     7501 cttccataag ccgcgtacgc gcccgttccg ccctcaattc ttcacgctcc agcgccatgg
     7561 ccagctgcgc tcccatgctt tccagcaaat ccttctgccc ggccgtcagg cactcccctt
     7621 cctctggcct tacgcccagt acgcccatgc aacgctcccc ggacatcatg ggaaggtaaa
     7681 acccttccgc taccgggatc gtatccgtaa accttcctgc ggggcggcgg tgttccatac
     7741 accagactgc cacgctccat tccttctcat ccatggcgta ctctccgccg gtctcccaaa
     7801 gcctcagctt accgccgcct ccttccggcg tcatgacagc caggcgcacc cccataatct
     7861 ggctgatctg tcccgccgcg acggaaatga gggaaggcac gtccgaagcg gatccaatgg
     7921 cccgggaata caggaacagg gcattggtct ttttttcccg ttcccgttct tcccgctcgc
     7981 ggatcctcag gcgggaagtg agccgtcccg tagaaagggc caccaaaaag aaaaagaagc
     8041 acatcaaggc gtcctccagc cgctctatct tgaacgtcat caccggggga ataaacagca
     8101 ggttccacaa caggacgctc agcgccgcgg ccagaagcat ggcccagcgg gaacggatga
     8161 aaaagcccag gcccactact ccggccaaat acaggaaacc gggagcaaaa taaccggtaa
     8221 atgcggaaac cagcaggccg gcacccgtga cggccgctgt cacgcctgcg gccagccagt
     8281 aatcccggcc atctttctcc gttttccggt gccagctctt ccaacggtta cggcccggtc
     8341 ccgaagcggc gggaaccacg taaatatcaa tctggtcgct tcccttcacc agcttatcca
     8401 ccagggatat gggccgcacc aggccccaca ggtagctttc ctgcggtttg cccaccacaa
     8461 tttggctgac atttcttaaa aaagccatgc ggagcagcgt ttccgccaaa tcggaaccgg
     8521 gcatcacgat cacttccgcg cccagccgcc gcgccaattc cagattggcg tccagccgct
     8581 gctgctgttc aggaggcaag ggtacgcccg tatccacgga aagggctatc cacggagcgt
     8641 tcagggcata agccatgcgg cgcgtccagc gtaccagccg ggcggaaaac gggctgggac
     8701 ctaccgccac catcagacgt tccccgctgc gccagataga gcggtccccg gaaatggtac
     8761 gcacctccgt cagttcatga tccaccttct ccgctacgat gcgcagggcc agttcccgga
     8821 gggccgtcaa attggactcc ttgaaaaaat gatccagagc ggcggaagcc tggggagcgc
     8881 tgtacacttt cccctcccga aggcgcgtcc gcagctgatc cggagtaatg tccaccagct
     8941 ggatgcaatc cgcctccgcc aatacggaat ccggcacggt ttcctgcacg ggggcggctg
     9001 taatgtcgtg aacggtatcg gaacggcttt ccagatgctg cacgttcagc gtggtgtaaa
     9061 cgtctattcc cgcggccagc aaatcctcca cgtcctgata gcgtttcctg tggcgcattc
     9121 ccgggacgtt ggtatgcgcc agttcatcca ccagaataag ctggggcttc acgcgcaatg
     9181 cctcctccaa atcaaattcc tccatttcca ctccccggta aaccatcttt ttcatgggaa
     9241 ggcgcggcag cctgccaatg agccgcatcg tatcttccct cccatgggat tccgccacgc
     9301 cgataatgac ttgcacgccc ttgtccgccg cctgaatggc cgcctccagc atggcgcatg
     9361 ttttgcctac tcccggcgcc atgccaaaga agattttcag caagccgcgg ccgggacggg
     9421 cttcctccct ccggatggag gccagaatcc tgtccgcccg ctggctgtcc gaatgcgttt
     9481 ctgaaaacat gaaaagagga atcggaatga tatccggctt gaatgttgga atcaacctaa
     9541 tggaagaacg ttcagggcgc aaataaaaca acccggaaaa aacattaaaa ttttattaaa
     9601 tcctctcccc tgttttcagg ctctccccac cccttcctct gccaagcatt cctgaaatct
     9661 ttttttcatg attttcccct tccaggttcc gcaccgtatc tctaccaccc tgcctccgtc
     9721 ctgtttttcc tgtgtccagc cattggaagt tactgttccg gaggcattga aaaaagcggc
     9781 ggcagccctt cctgcgcttc aaatcccggg gccatccctc ggacaaacct gaaagcttcc
     9841 tccatccggg gaaaagtctg acaaatgaaa aacgggaatg tttccccctt tcctaattga
     9901 caaacgattt cctttcttct taatgggtta atgtttatca atccggtaca ttgtctgcca
     9961 gcctctctct gcgcatgcgc gttgcttccg gcaacaatcg cctccctgca tgcggaaacg
    10021 gcttacagta cgccctgcgg ctatatccag acaaccctgt acgccaaagc aacgggttat
    10081 ctgggtttag gggtgcatcc ggaagccttg gtacagcaca cccttgaaga aggcaacatt
    10141 accatttccg gtaataaagt caccatcacg gacccggacg ttaatttcaa cgccctgatg
    10201 aatgaaaact ccgcctatgt gctggttctt cacttcggca atgaagttgt agctcttccc
    10261 ttaaaccggg atggttggaa aaaaccggga gccagctgga cggagcattc cattaaagtg
    10321 gatgaccgcc ttctttctga tctagtaagc aagggcactc cccccgattc atatgtcctg
    10381 cgcaaggcgc gcactctgga tgatattctt ggagaagaca accagtttgg cctgaaaagc
    10441 ggctccgcca tgtctgcaga cacggttaat atctttaatt cggcaaccac caagcttgcc
    10501 gcttattatt ctggaaatga atggagaaga aggggatcta cggaaaatat cggcagtatg
    10561 ccggtgttca gccatgaacc cctgaccatt acgcgcaaag ctggttccga tgttacagct
    10621 gtggtcattg gggaagtatc cctgaagaat cagaagctgg tcgttcccac attcaattcc
    10681 cttcttcaca ctcgcctgcc cctttcccag actttggctg aaatagcttt gcaattgcct
    10741 gatgacgccg tcatcaacat tcccgcagat gacaaaaatg ccatggtcaa ggtcgtcaac
    10801 aacaacggtt cctggaaacg tcaggacaat ggagaggatg tttccaagat ccccttcagc
    10861 ggagtttttt ccatctacta cgaatcttcc aagacacctt cctacatcac ggtgtccagc
    10921 aaacatcaaa ccaataaata accagccatg ttctatcgct cccttttcac caatgctttc
    10981 accgcctgcg ctctcttttc tggagaaatt gccttagcgg acagtttcaa tatcagcaat
    11041 gacgattcct ccgcatattc cctaatcaat tctctcggag aaccgctaaa tgcaggcaat
    11101 gccattttag gcaccttcag cagcggtact tggagttatg atgtgaccac cagccgatgg
    11161 aacttccaag gcaattcgta tgcacaggat caactgaaat acagtgatct gatgagcatg
    11221 cagaacaact tcaccagcgc gttgacaaat ataccggaag agcaaaatgg cggcgtcgct
    11281 gacggcagat ttaacttgtc cggagattac tcttcttccc tgacatcccc ggtttatcta
    11341 atggtgacgg gacaggatgg agaaacagct ctttttgtct ttaaagacgt atccagcgac
    11401 agtctgctta attacgataa tatagatata tccggcaccg agacgttcga tctgtggctt
    11461 acgaccaagg agcaggctct aacaaacgag cttgactact acaccgaatg catcctggga
    11521 aacatcaatc tgcaaaacaa taccatccag cttttgatcc cggaacccgc gacagccacc
    11581 atgagcttgc tgggtttgac ggccctgatg gtacgccgta aacggaaata gaagcttccc
    11641 ttttcatcaa acccctgtac gggtattttc cgtacagggt ttttttgttc tgccaattgt
    11701 tcatccttca tcttcttctg cattttccct ccagaaaacc catggagccc ggcaataaaa
    11761 ccgccccgcg cactcggaaa gagcggcggg gcgcctgttc aaacggtaaa aaccgcagga
    11821 gttatttctt aacgggaatg atatccagtt ccgctacggc ggcgtaatcc cgcccctgcg
    11881 cctcactaag ggcggaaaaa cggaaataac gcgccttgac gggagcctgg aatttcactt
    11941 cctgtaggtc agcactgtcc gggaaggagc ccttggcggc aggctgccat ttctctccgt
    12001 ccgtgctcac gtccacggaa taatctttca cgcggccatt actgctgcca tcctgacgtg
    12061 gaagataagt gattccggag aattcccgct ccttacccag gtccacggcc agtacatgcg
    12121 gatgtttggt cacggtttta ttgtattcag agtgccagaa ggtgccggga tcaccgtcaa
    12181 tggcgaattc ggcgtccgct tcgccctgct cctggctggt tacgctcttg accgtatact
    12241 tgtccgtgcg ggtcagcatg gtcatatcca gccgggccgc aggcacgcgg attttcccct
    12301 tgtaggactt ggcggtaatg ggccgcataa agaaggacat cgggtagggc ttgttggcct
    12361 tgatgatgtc cctgcccatg gggccgggac cgcaggaagc accccccagg ccgcgcgtgg
    12421 cggaagagag aaccagaacg gtcttatccg tcgtcttcgg cagttccacg gggtggtttg
    12481 ccaggatcaa atccgtggtg gtatacggaa gggcggagaa agcgaacggt gcgcccacgg
    12541 aaccgaaaag caggccgttc cccttgtcgt ccgtaacggc cacccagcgg gtatcctcat
    12601 ggttgccgca atcctgcggt ctgccgtacg ggaagaaaga atcccaggct gtcgttttat
    12661 acacgcccag aggcatgccg ctcttgcggt ccgcatagtt ttcttccggc ccgcgcccgt
    12721 aataggctac gttgtccata ttcgccggca actggagttc gtatcccagg cgcagcagtt
    12781 ccaggggatt gccgcgcgga agcagtacgg actggcagac gacagttcca tccgcataga
    12841 tggtccattc caaattattg atgaaatggg tgttcgtatc gttcaggggt tgcttcctgg
    12901 ccgtgatggt ggtcttggta tcgccgtagc cgctgatgtt ttcagcctgt ttgccgctta
    12961 ccttgatgga ttgcttgacg gtgacgctat tgccgttatc aatggcttcg taactgagca
    13021 gttcatgctt cagttcacgg aggccgttag ccatgctctt ggccatgacg cccggctcat
    13081 tgctggaggc ggcgcgcagg gcgttcaccg ccatgggcgt tttaaacaga ggcttgccgt
    13141 ttaccgtgaa ctgggccagt tccccggtgg ccgcgtcaaa ctgcacggaa aaatccttgc
    13201 cggaaacggt atgcttgtcc gtgctgaggc tgactttgcc cgcaggagcc ttgaacacag
    13261 gcagatctcc ctgcacggga agctggagct ggtcaaaggc caattcatat cccttcttgg
    13321 cccaccccct gtccttcttc agcttgtaac ttatgcgcaa ggcatactcc acccccggtt
    13381 tccggttttt caactgggga atgtccggaa tgggaaccac aatcttttct ctgggaccgg
    13441 ccggaggcgt gtccagcctg ccttcagcca ctgtattgcc gttttccgtg agggtccagg
    13501 taatgtcgta tggagaaagg tccgtaaaga aattcttgtt gaaaatggaa atcctgccgt
    13561 cgtccgtcaa agatgtggaa atattctgat agacgtgctt gacttcaaaa taacccggtt
    13621 ccggcgtacg atccgacagg atggtgccgt tgaacacaaa ctggccgcta ttgggatggt
    13681 cgttgaaatc gccgccgtag cagagcatct tttctccatt cggcagggtc ttgtacaagc
    13741 cctggtccac ccagtcccag atggcgccgc ccataatgcg gtcggaagac tcgatggcct
    13801 cccagtaatc cgccaggttg cccatggcgt tcatcatgtt gtgtgcatac tcggaaatat
    13861 agtagggctt gggaaaatcc ttgttgcccg ccatggaacg cgtccagtcc acggacggat
    13921 actggttgga ccccaggtcc acaatgtcat tattgcgttc gtagtgggtg gggcgggaca
    13981 tatccctggc cttcaccatc ttttccgcac tgcggaaatt ctggccgggg ccggcttcat
    14041 tccccaggga ccacataatc acgcagggat ggttcttatt gcgctccacc atcgccatga
    14101 tgcggtccac atgggccggc atccactcaa tgggatggga aagggactcc ttgccatagt
    14161 aatagccgtg ggactcgatg ttggcctcat cctgcacgta aatgccgtac ttgtcacaga
    14221 ggtaataaaa ataaggatcc gcgggataat gggaacagcg cacgtggttg atattggcgc
    14281 gtttcatcat ccgcacttct tcctccatct gctccggagt cacgtaatgc cctgtttccg
    14341 gatggctttc atggcggttc acgcccttta ccttcaccgg ctggccattt accagaaaca
    14401 cgctgtcctt aatcaccaca ttgcggaaac ccacgcggga ggaaaccatt tcttccgtct
    14461 tcccgtcacg cttcagcgtc agtaccaggg tgtacaggtt gggatcctcc gcagaccata
    14521 gtctgggttt ggaataaatg cccagcaggg aagttttaaa atccttcata ccggtaatgc
    14581 gcaagggctt ttccaacacg ccgtcatatg gcgcatcctt gggcttgaca ggttctacca
    14641 gttttccggc agcgtcatac agggccatgg aaaccgtgca gccgtccagc ttttccgttg
    14701 ccggaaacag attgcgaaca tccacatcca cctgaaggcg ccaatcgccg tccacggtcc
    14761 ccggtttggc atggtcaata ttcaaagccc aatccctctg gtccaccgga ttggcctgtg
    14821 cgaaaaagtc gcggatgtga actttcggca gcgcaaagat ggaaacattg cggaaaatgc
    14881 cggacaggcg gaacatgtcc tggcattcca gatatgctcc gtcggaatgg cggtacacct
    14941 ctgcggccac cacgttctcc cccttcttaa ggtaggggct gatgtcaaaa cgggccggat
    15001 tccgggaatc tttggaaaaa cctacatact gcccgttgat ccagaggtag aaaaaggaat
    15061 ccactccgtc aaactgcatg tacacttccc tgccgtccca gtccgcaggc acttcaaaag
    15121 tgcggcggta ggaacctacg gggttccgtt ccttgtagga cgtataattc ttgttggaag
    15181 gctccttcat tacataaggc caggagcgtt caaagggata cggctgattg gaataaatgg
    15241 gggtgccgta accctgagtc tgccaggagg aaggcacctt aatctccttc cagtccttta
    15301 cgtcgtaatc cggcttgtaa aaatcggccg ggcggctctg gggatccttc gcccagtgga
    15361 acttccactg accgttcagg gacctgcgcc atttactgtt ttcaggcagc actttcaagg
    15421 cggactgcaa atcgctgaaa gacgcgaaaa aggcggttgg agcttcctta ttcaaatgca
    15481 ggttttgttc ctgttcccat tccagccctg tgggggctgg cgcgggggcg gaatcgcccg
    15541 gtgctcccag gccgcacatg gcggaactta ctgccagtgc ggtacaggat aaaagccagg
    15601 atctggtcgt tctcatgaaa tcaatatgtg tcggagttga tatgcacaaa cggattgtcc
    15661 tgttacatac gaatccgcaa ttctaattct aacagcaaac ttgccctttg caagctgctg
    15721 ttgcgtttat gaacggcctg caaacgcagc cgttaaaaag tatgccggag ctggagaacg
    15781 cccacacact gcatggattc ctgctgggtg tattccttgg tcatgtaatg caggccgaaa
    15841 agcaattccg agcgcttgta ccggaggccg aagccataac cccattctcc cactacggga
    15901 tacttgttca catacttggg ggaggaacga aacaccgtac catccagata caggtcatga
    15961 gccacggctc gcacggcagc atgaatataa ccgtaatacc cccaattgga aacggacatc
    16021 ctgttataaa cgaagggagg cgctgcgctg gtcgcgccgc gaatgctgtt ctccggagaa
    16081 gtattgccca gattatagcc gaagcggaag gacatgccgc cccccgcctg cacctttact
    16141 gtgccggcgt ccgcatgcca gtaagccagg gcatccgttt caaaaccgga gccgtagcgc
    16201 ttctccagtc cgcgcaggcg gtaataccgc ttgaaaaaca aattggcagt catctctccg
    16261 gggagctggt tggcccagcc gggccattgc tccatacccc acagcttatg gatgaaatgc
    16321 tgggagccct tggcgaggga attcgtgccc gtggtgccga gctgcagctc cagggaattg
    16381 gcccggtctt cattcttgac cagggaggtc agccccagcg tcaggttgcc cacatagggg
    16441 tgctggttgt agatgggata ggtggccttc tgctccgggg tgtacatgag ctgggtcagg
    16501 tccaggcccc actgctggcg caggttggcg gaaccgctca tgcccatgaa ccgccgccag
    16561 gaatccgagg cgttcgtgcc gcccagcact gttcccagca tgccggaaaa cgtatggctc
    16621 cgggacgtgg tgccggacat ccacgcaaag cggacgccgt tggtataatt atcatcatcc
    16681 cccacgaaca tatcgttttc cagatggaaa ctgataacgg agccctcctg cggcgcttct
    16741 atctgctggg ttcctgcctg caggaagccc accggcagca gggcggcgca catgcacaaa
    16801 gccgtacatt tcatgcgggg cagtttgtgc gtttcacttc cgcgtggcaa ggaaaaatca
    16861 ggcatgctct gcgtttgcac ttttcaaagc cggcaaatat ccacaaactt catccgttcc
    16921 gccacagtcc cttacaccat gattattgac acgcactgcc atctggcttc cgctcaattc
    16981 gaccagtccc gcagggaaac ctacgtccag catgccctcc gggaaggcat tgaccgcatg
    17041 attacgctgg gggcgcggat ggatgactgg gaggcaaaca cggcgtgggc gcgccagttt
    17101 cccggcgcgg ttttttgtgc gctgggcatc catccggacg atgcccatga cgccccggca
    17161 gactgggcgg accagctttt ccggaaggca caggacgttc ccctggccgc catcggggaa
    17221 acgggcctgg attacttcca cggggtgccc caaggctggg aaacggaaca attccaccgc
    17281 cttcagcagg accttctgga acggcacttt gacctggcgg agcgcctggg ccttaatatc
    17341 gtcctgcata cgcgggaccg gaaaggctcc gcgagctttg aggacgcctt ggctattgcc
    17401 cggaactacg cgggccgcgt gcgtcccgta tttcactgct tcatcggaaa taccgctcag
    17461 gccatgcgga tctttgacga actggacggc atggtctcct tcaccggggt ggctactttt
    17521 aaaaacgccc ccgtagtggc ggaaaccgca aactggtgcc cggaaaaccg tatcatgctg
    17581 gaaacggact ccccctatct cagcccggaa cccctccggg gccgcatgaa cgaacccgcc
    17641 catctcattc atacagcccg tttcatcgcc gctgccagag gcatgaacct ggaagatctg
    17701 gcccatgtga cgacccgaaa tgcggaaacc ttttataatc tgggcaaggt ctgaaaaaaa
    17761 atcccgacaa tccgcgctac cgccttgaat ccgaaatcat aaattagtat gaacagggca
    17821 cacatgaaaa cgccattcat tttaacctgc gcctgcgctg ccgcgctggt attccccctc
    17881 tcttcctgct ccacccagaa ttcctccacg gccggttacg acactgcgga accggccagc
    17941 ggcgaaatcc cgccgtggat cgcggaaagc tctgatcccg cttacgaatc cggccattac
    18001 agcccggttc aaagttcttc cagctacgct tacaatcccc ccgccaagcc ttccgtcact
    18061 aaaaaatcat ccgcgcggaa gagcacggcc ggcagcaaat cctcccgcag cacggctgcc
    18121 agaaagagct cctcccgtaa atccgctccc aaggcccgca cctacaccgt gaagaaaggc
    18181 gatacgctgg gagccatcgc ccgcaggaac ggcacctctg tcaaggccct taaaagggct
    18241 aacggcctta agtccgatct gattcacatc aaccagaagt tgacgattcc gcgttccaag
    18301 taatccgggg gatcctcccg gttccaccac ttatgaagcc tctccttgtt ctggccgcct
    18361 tccccgtggt gctcatgggg tgggcgtcct gttcccactc cggcacgggc catgcggccc
    18421 tgccggatcc gcgtgccgtg gacaaatccg ttccggaata taaaaacccg tttcatcctt
    18481 ccacatacgc tcattttgta gcccgcaagg actaccggaa aacgtacgac gtctataagg
    18541 acgatacctt gctgaaccgc cagccgaaaa aatcccgaaa aatcttcgtc tgcctggacg
    18601 aacaacgggg ccagtatctg gtggacggct tggtcgccat ggacttcccc ctctcctccg
    18661 gcgtaaaagc ctatccgacc aaaacggggg actacgccgt tatctccaaa aaggaagacc
    18721 atgcttccaa cctctacggc aaaatgtatg atgccgaagg caaatgcatc aactacaatg
    18781 ccgaatccac ggatcccata ccggagggag gtcgttttga cggctctccc atgccttatt
    18841 ggcagcgtct taccaacgcg gggctgggcc tgcatgtagg gaaagttcgg cggcatcctg
    18901 tttcccatgg ctgcgtccgt ttgccgcgaa atgttgcgga aatcctctac aggcaaacca
    18961 gcataggaac gcccgttacc atccagaaaa cgccgctccc cgtgccggaa gcgcagaaaa
    19021 tccctcccgc acaacaacag gaatccggag gccgcaaaca atagcctgcc gggaaaacgc
    19081 tcccttcaaa acacaaactg ttttaaaaag actgcccgga aagcagtctt ttttctatcc
    19141 cattttttcc aaggcattaa catgacgtat aaaaaaatta tatttcctaa attattgcac
    19201 tatttttacg gccacacaac gaaaagaagc gttttgtaat cctcatcgga gggcaaataa
    19261 aaatgccgga taagatgact gggtaaaacc aatcacctta tccggcattt tttgtttccc
    19321 ggcaaaaaat agccgtaaga ataacagaca catatgaaaa gaaatgcaat tgatttaggc
    19381 acactggacg tatgcacatt attcagaaag tattttatcc cgaccttatt cgggatgctt
    19441 agcatatcgg cagtaacggc agtagacgga atactcgtcg gccacggaat cggaagcgat
    19501 ggcattgccg ctatcaatat atgcattccc ctgctgatgc tgtttaccgg gatagggctc
    19561 atggcaggtg taggctcatc cgtagtcgct tccattcagc tatcaaaagg aaaactggga
    19621 gcagcgcgcc ttaatgtcac ccaggccttg ttgttcgtca ccattgccgc catcgtgcca
    19681 tctgccttga tgatggcata tccatcagct acagcccgat ttttaggcgc ttccgaacat
    19741 cttaccccca tggttaggga atacctgttg tggttcatcc cctcgttaat attccagatg
    19801 tggtgctctg tctgcctatt catgattcgt ttggatggcg ctccgaagtt ggctatgatg
    19861 tgcaatatca ttgctgcaat catcaccgta atactcggtt ggctgtttat ctttccattg
    19921 ggctggggat tgacgggagc tgctttcgcc gcaaccatag cactgtttgc gagcggcgcc
    19981 acgggcctta tttatttatg gaaatttgca cataaactgc ggctttatcc tattaaatgg
    20041 agcatcagaa gttttcggtt ctccctcctt aatattgtct gccaatgtcg catcggttct
    20101 tccgctttgc tgactgaagc gacactggcc acgctcatgt tcgtcgggaa ccacacattg
    20161 atgaaatatc tgggagataa tggagtagga gcgttcggca ttgcctgcta ttaccttcca
    20221 ttcgtattca tggtaggcaa tgccatagcg caatcagcgc aacctatcat cagctataac
    20281 ttcgggcttg gagagaaaaa acgcgtagcc gatacaaaac gaatcgcatt atttacctcc
    20341 gttgtttgcg gcatagtcac gacggcagca ttcattttgt tcccgcatat tctggtaggc
    20401 ttgttcgtca atccagacag cactgcggcg ctcatagcca ttgagggatt tccatatttc
    20461 tccctggcat ttgttttctt catcatcaac ctgatgctca tcggttatta ccaaagcgtg
    20521 gaaaatgtaa aactcgccaa tttgtttgcc ctgttgcgag gatttgtttt tcttattcca
    20581 tgctttatcc tgttgcctga agcaatggga acgaacggtg cgtggctagc actttgtctg
    20641 tcggaaatgt tgaccatatt aacagttgct gtcctattta ttctacgcaa aaagaaaaca
    20701 cttctgcatc aaacaggtga agaatgcagt taaaaagatt gttctcaggc atgccacgac
    20761 acgcgcataa aatacttgca agcgaatata gatgacttag cactttgtct tccctgaact
    20821 ggctacagct tttaaaaatt attaaacaaa agatatttct ttaacaaagt ttttctggga
    20881 aaacgacagg atagggatta ccctctgctg cgagaaaccc taagattttt gttatgcctg
    20941 catctccgga gcgcattctg gaaaatttgt tcccctttta ccggcaggaa gaatttcccg
    21001 ttctcctgca ccagtataaa acgtggtcgg aaagtcgccc tctggaagga tgccggatta
    21061 ttgacgcttc tcccgtgttc cggaatacct gcgccaaata cgcctctctt ctggccgcag
    21121 gagcggagct gacagtagcg gtctccccag taatgcccta tgacccggaa acagccgtat
    21181 tattgggaca gtatggaatt cccgtggtgg aagcggaacg ggcaggcgga ccttatgatg
    21241 tggtcatgga ctgcgccgga gcgctcagcc atgttcccag ccgctgcggg tatgtggaat
    21301 taacccgctc gggtgcggaa cgctatgcgg catgcacgca gcccgtgtgg atggtggacg
    21361 ccggaaagat taaacagata gaaacctgcc ttggaaccgg agaaagtttt ttccgggctc
    21421 tggaatctct tggaataaaa aatgaacagg gacagactct ggtaatcatt ggacatggaa
    21481 aagttggccg cggaatagct ctgcatgctc tcagacggaa actgaatgtc attgtagcgg
    21541 acgtggaaaa aaaacctctg gcttccgcct cttttgtgcc ggcaacggac aggaaaaccc
    21601 ttactgacgc aatcaggcat gctttctgcg ccgttacggc tacgggaaag cggcatgcca
    21661 tgtcagggct tattgagccg gccatgcctt tctcttccgg cgttctgctt gccaatatgg
    21721 gtgtggagga tgaatggggg ccggaaattc cgaaaaaccg cctgctgaac gaaggccgcc
    21781 ctcttaattt tattctgccg gaccctacgc aaatgcgtta cattgatcca cccctggctc
    21841 ttcataacca gggagctctg gaacttgccg aaggccgagt tttgccaggc ctttttcctc
    21901 ctccgccgga aatggaagag cattttcttt ccctgatacg ttcctgcggt tccgtaccgc
    21961 ctgacatgct gaactggatt tcctgactta tcaccttgtc tttgccggga taaagcggta
    22021 tcgtgtagaa tacggtcggg aacttattgt tccatggtcg gtttgacgtt tcattgactt
    22081 cgcaactcat gaacacttat tatttttacc gcagttatcg gctgggaggg agaatcctcg
    22141 gaaccctggg atgcagcctg gcctgggctt ttttcggttc cggctggtgg aactttcttc
    22201 tcggcgccct tttactgttc ctgtggcttc ccgttattat gggcaggcct tattttctca
    22261 caatcacttc ttcttccatc ttctggggca acagcatttc acgcagggaa ataccgtggg
    22321 aattaattga acgcatagga tgcacaccaa ataatgaggc agaacccgga ttgatgatta
    22381 aactgaagga cggacgtatt ctggatgtgg aacattcctg tttcaattcc atatcctccg
    22441 tccgtgaagc cattcttcaa gcatccggtt tttatccctt tcatctcgct ccggacttgt
    22501 tcgctcctcc gggtctgctg ggcgagtgat acggagtaaa atttgtcaat tctccgctct
    22561 gaatatttgc tgtccccgca tcttctcatt cccggataat ggcattctcc cgtcgtcgca
    22621 gaggtttccg acgacgcccc tcgcgcccca tgcaggtatt ccgtcttttc aaggcagatt
    22681 acagttttcc tgtaatggaa aataaaaaat gttccacgta gaacatttat gtgaataaca
    22741 tttcttttcc attcactctc agccaataaa aaacaggcgt catgtctcca cgacgcctgt
    22801 ccctctgtat gaacgatttt gtgccaccaa aatcaattgg gagatctcat cggcatgagc
    22861 acatacagga actcatcgcc aatcttcaag acgcccggac tcatttcatc aatgaggttc
    22921 agcgtaatga ccgtatcccc ggccatcgtc ttcaggggag ccatgaagaa atccggatta
    22981 aaggaaatgg aaagcggcct tccggaataa gtgatcgtca tcgattcatg agcttcgccc
    23041 actccttctg cgctggaagc cacttccagt tcgttttcac gaaaattcag cttgatattt
    23101 gtgctcttat ccacggaaag gagagaaaca cggcgcactg tctccagcag ttccccgcag
    23161 ggaagttcga tactttcctt gcaatccgta ggaattacct gccggtagtt cgggtaattg
    23221 ccgtcaatca gtttgctgat taagagcgta tcgttgattt caaaggctgc ctggttgcgt
    23281 gtgaaccgga tcgtgacggt tcccacttca tccagaaggc ggcggagttc tccgacagcc
    23341 ttagtgggca ggatggcatc cagttgctgt tcttccggaa attccagggc ggtttccgcc
    23401 aaagccagac gacgtccgtc cgtggctacc aaagtcattt taccattgcg gaaagaaagg
    23461 gaaatgccgt tcaacacgta gcgggtcgta tccgtggaaa tggcatattc cgtccggcgg
    23521 atacagtcac gcaacatatt ctgctccacc tggtacacag tagcctgctc aaaagacggc
    23581 agttccggaa attcatccgc cggaaggccg ttcagtttaa actgggagcg gccgcattcc
    23641 acagcagcct ggttgcgttg gttgacatcc acctggactt cggcttcagg aagttcgctg
    23701 atgatgctca ccagcttttt agcgggcagg gtcacggtgc cttcacgcag cacatttgcg
    23761 ggaaccacgc cagtaatgca tacatcaaga tcagtagccg taaaacgtat ctggccgttt
    23821 tcagcttcaa tcttgacatt ggaaagaatg ggcagcgtcg tcttggaaga cacaacgctg
    23881 gcaacctgtc tcagcccgtc gagaaatact tgcttggaaa tggtaaactt catgtgtctg
    23941 tgcttttatc tgcctcgcat cttctcataa gtgcctttca tggttcaagc catttctccg
    24001 tcaaacccta atatttggca gtattttttt aaaagcatac tatatatagt aatatgcaat
    24061 ttctccgggc atatcttgca tgaaaaggtt tttcaaatta tttaagcatt cttaaaaatc
    24121 cattccgggg aatcagattt cttcaatatc cttgtgcgga ttataaatcc ggggtcaaat
    24181 tatttttctg aaaacaaatc atttaacgga aaaaagatta cggagaagat aaaagaagag
    24241 aaaacagaaa cggcattcct gttggaaaga aaacgtacat ttccaataaa attgccgcat
    24301 cctaggaaaa aagagacaat atccggattt ataatccgct accctcctat cggattgatt
    24361 ttaacccata tttcagaatt tccgtgaaac gctcatttat tattttatct ttactggcag
    24421 cggcaggcat ggcccatgcg gctaccccta tctattcctg ggaatggact gcggaaggag
    24481 cacaaataac cgcccccaac tggagcggtt ccttcaatta cacttccggc aatgattacg
    24541 ccacgttcgg cagcagttcc ggcactccct ataactcaaa cataacgggc atccaaggca
    24601 gtttcaccgt gagctttgac ctgaaaaacc tgagttccac cagcaatggg tggaaagaca
    24661 ttttcagcct gtacaccaat ggaacggtct ccggagaatc caacagcatg gtgctggaat
    24721 tcactaataa cggagaacta tacctctaca ataaggggtt cggcggccaa actggcggaa
    24781 atattaatac gggattgacc tggacggatc tccagcagga cagctggact aatctgagca
    24841 tcgtttccga tctttcctcc caaacgcttt ccgtgtatgt caacggcgct ctggcaggta
    24901 cgattaccga gtggaatcca tcctcccccg ccctgacagg ctcccagttt ggagcttctt
    24961 ttggaaatac ccgccccatg aacggcacta tggatatcaa caacatcaaa ttttatgacg
    25021 gcgttgtgct tcctgtgccg gaagcagcca cagcttccct gggcctgttg gggctggctg
    25081 ccctgatgat gcgccgccgc cggtgctgct gaagcaattt taaccattcg ccaaaggctg
    25141 catgcgggaa gacgtttttc cgcatgcggc cttttcagta ttaagagaat tcctttaccc
    25201 gattcatgcc cccgtgaaga cttcccccgg tctggtccaa gcaccgggag gaagaacccg
    25261 ccccggcaaa acgacggtat tggcacccag ccgaacatgg tctccaatga cggcgccaag
    25321 cttgtttctt ccagtaaatt ccagctttcc tcccgccacc attctgtgtt cgccgccatc
    25381 atggcgaaaa ttggaacata ccgttccggc gccgaaatta acgtcactgc caataatgga
    25441 atcccccgca taactcaaat gaggaataaa tgtgccgtcg ccgatgatac aatttttcag
    25501 ctccactccc tgccctacta cacagccatt ccccaaagat acacaacccc ttaaatagca
    25561 attaggccct atgcggcagc catcacccgc ccacacggct ccctctacaa ccacgccggg
    25621 catgatgacg gttccttccc ctacccggag cgttcccatg acataaaccc cttcccttcc
    25681 agagaaatcc tcccactcca tcttctccat cactctttcc tgccattcca gaagatccca
    25741 gggataaacg aggcgctcta tcgctgcttc cttccgcgtg aatacccgcg tcaacccgtt
    25801 ttctccgctt ttccgggcca gcaaatgccc ttcttcactc cttgcttccc ctgccccgca
    25861 aaaggcaagc catatggccg cctccgggga aaaatcatgc atggacactt ctatgatttc
    25921 ccctttttct tctgaaaccg gcgttcccgg gtcaaaaacc gcttcaaagc ccacttcccg
    25981 aaggcgggaa gcacagtaag cggctatatt cattccagcc acggaaaagc cgtagaaggg
    26041 cctttcaggg cttgtagcgg gaaaagtatg atccctctcc ggaatgtaaa tcaatctggc
    26101 agccttcaca aaaggatggt atgctttcct ccgggttttc tgcaagcagg aatgccgttt
    26161 ctgtttacag ccgcacctgc caataacctt gtgaaaggcc aggttgatta actgtgagta
    26221 tgatgtgaat aacttattca gatattatcc acaggcttgc gggccaaatt tattaacatt
    26281 tgaaatgatg ctcaatactc tcatttacag ctatttgaaa tcttattcac actgggcaca
    26341 cagcagaaga agaaggaatt taatattgtt aaacctaata ttcttcaatc acacaacgtg
    26401 attgtccttc ttccggtttt catgctacaa aacggcgtga atacgaaaaa tcaggatgac
    26461 tgttggatga acatggccat aaaactggcc tcaaatggaa ttggacttac gagtccgaat
    26521 ccctgcgtgg gggcagtcat tgtgcatgaa aaccaggtga tcggttcggg atttcataaa
    26581 aaagccggct tgcctcatgc ggaaagagag gctattgccg acggagtggc aaatggcaat
    26641 gcctccctgt tcgcggaatc aactttgtat gttactctgg aaccatgcag tacttccggg
    26701 aaaacaccgc cttgtacgga tgccattttg aaacatcgtt tcaaacgggt ggtttatggt
    26761 tcggaagatc ctaatcccaa acaccgcggc gctgcggctg atattcttga acaggccggc
    26821 atcaaggtta cccgcggggt tctggagaag gaatgcgatc atttaatcag ggggttcagg
    26881 gtgaacatgc tggaagggcg tccctgggta attgcaaaaa gcgccatgtc tctggatggc
    26941 cgcatttccc gtagtccgga acgttcccaa tggctgacga atgaaaaatc cagatctttt
    27001 gtacatacct tgcgtgccga atgcgatgcg gttcttaccg gagggaatac cgtgcgtctg
    27061 gacaatccgt ccctgaccat taggaaaccg gatcgtcctg tttctcccct caaggaacaa
    27121 ccctggcgta ttattctaac ccatgatgct tcttccatac ctgcggattc catttgttta
    27181 acagatgaat ttcgtgaccg cactttggtt gtggagaatg ttttcaatta tttggaatta
    27241 ttaaaaaaat tatataatga taaaaaaata ggaactatcc tgctggaagc gggaggctgt
    27301 ctggtccggg aatttctgaa agccggttta gtcgacgagt gggtggggtt ttatgctccc
    27361 atcattatcg gcggcggtac ggacggcgta gcgcccggtc cgtttctgga gcatgaagcc
    27421 catcttgaac aacctgttgt gaatattttt ggagatgatg tatgtatccg gggagatatc
    27481 tgttaccggt aatggatgag gtctttgttt attggttatc cgaagaattt taaacttggg
    27541 aaggtaattt tcattcgccc ggtggatttg tcattcagcc cttccggcct cccttgcctc
    27601 ttggagtaag ctcaatgctg ctttttctta tcggcagtat ttgaatagaa tccgtgaaga
    27661 aatcgtgtga cgcttcttaa tgatttgcga tggataaatc aggaaggccg gtattcaata
    27721 atactgaagg gatattccat ttctttttac gacaagcaga aagtggtggt tgttcggagg
    27781 aagggaagag ctgctgtcgc gcaaaaagac attcagaaaa cagtattgaa tctttttatc
    27841 gactccaggc aatagcgaca gggaaatata atgggatcat atacggctca aggatgtccc
    27901 tgccatattt atttttcaat acccgcctcc tatggtcctc cttaaattga aatttttgaa
    27961 taaaaaacgc ggggtattat gtttgttgcc tgctgcgttt ttgcagatgc acaagcaaca
    28021 gggcgatatc tgccggggta attccgggaa tgcgggaggc ctgcccgata gttgcaggac
    28081 gtatgttgcc cagcttgatg acagcctctt tttttaaggc atggacatgt tcgtaatcaa
    28141 tatcggaggg aatagtttta ttttccatcc gtatgatttt ttccgcctgt tttttctgcc
    28201 tttcaatatg cccttcgtac tggatatccg tagcaatggc atccaaatgt tccacgtgga
    28261 acattttttt cagttcttca ggcaatagag tccagtcatt gccgggcctt ttaagccatg
    28321 tggagagaga aactccttca tgtttaagcg cggcagcttt tgttattcct tcttcaacgg
    28381 cgtttttcct gtcttcagcc ttccgggcca tttcacaggt aataaggccc gcttcaaaag
    28441 ccagaggggc caaccgcagg tctgcattat cctgacgcag caacaggcgg aattctgccc
    28501 ggctggtaaa catgcggtaa ggttcatcgg ttcctttggt aacaaggtcg tctatcatga
    28561 cgccgatgta agcctgatcc cggcgcagca ccagagaagg tttgcccagg gttttcagag
    28621 cggcgttcgc tcccgccatt agtccttgcg ctgcggcttc ttcatagccg gaagtgccgt
    28681 tgatttgacc tgcaaagtac aggttttcca ccagttttgt ttccagcgta ggatgcaatt
    28741 gcgtgggagg acagaagtcg tattccactg catatcccgg acggatcagc tgtgcgtttt
    28801 ccaggccggg aatggtatgg atgaagtcgc actgagcgga gtagggcagt gaagtagaaa
    28861 ctccgttaag gtagaattcc tccgtactgc ggccttccgg ttccaggaat acctgatggc
    28921 tgtttttttc cgcaaagcgc acgattttat cttctatgga aggacaatac cgggggccta
    28981 cgccttcaat gataccgctg taaagggggc tgaattttaa gttcttccgg ataatgtcgt
    29041 gcgtagtctg gttggtgtag gtgatccagc acggcatttg cttcatccgg aaattttcat
    29101 ctccccacgt gttcaaagtg caaaagggtt cggaatcacg tttcagtata tcacgggaac
    29161 ggtagctgaa cagggcttcc ggaacgtctc cgggctgtat ttcgcagacg gaaaagtcga
    29221 tggatcttcc attgacgcgg ggagaagttc ctgttttaag cctttccacc tgaaaaccca
    29281 gcatggcaag atcggcggaa aggctggaag aagcgcaacc cattcggcct cctgttatgt
    29341 gttgaaggcc cacatgaagc aatcccttca tgaatgtccc ggagcagata atcacgctgc
    29401 gggcatggat ggcgcagccc agagaagtaa cggctcccgt tgcctgctgt tctccattga
    29461 cgagcagttt gaccacatct ccctgaaaca ggcgtatgcc gggggcggtt tccagaacca
    29521 ttttcattct tgtgcggtag gctgttttat cgcactgggc gcggggagcg cgcacgctgg
    29581 gccccttgga agcgttcagc atccggaatt gaatggcggt agcatccgtg ttcaacccca
    29641 tcgctccccc catggcatct atttcccgga cgatatgccc tttggcaagt cccccaatag
    29701 ccggattgca ggacatttgt ccgatagtat ccaggttatg agttaggacg gcgacggaac
    29761 accccagacg ggatgctgcc agtgccgcct caatgcctgc gtgcccggcg ccgacgacga
    29821 gaacgtcaaa cagggggaaa acggaagaag gggaggagtt catcaggatg cggagcagat
    29881 agcaagacga tgcagaatca ggcggggatc aagagaggag gacaccaggg ctttatccgc
    29941 cttcatgcat tctttcaaag tttgcttcag ccgttccgtc ttgaatttgg aagcattctg
    30001 tgccgccaga aaaagcggat acgcattggg tgtaccgtct tttttcaagg gaattaacgc
    30061 ggccgcatag gagggcaggg aagaaatgcg ggccgtaaat tccttgaaat tggtgggctt
    30121 caggttgaat gcgtcacata aaagtctggc agccagcaaa tttcttaacg tagggatgaa
    30181 tgcggcacgc atgattgtga tggcgttttc accgcgttcc agttgaaaat caatcagaga
    30241 gatggccgca tcgcttttct tgttttccaa agcccgggaa atttcaaaaa taacgcctgt
    30301 acgcgtcagc ggaaccatgc gctccacatc ctccggcatt acggtgcgtc tgtccgctcc
    30361 cagataaaga tccagttttt caatttcgga aatgatttgg cgggaggatt cgctgacgcg
    30421 gtgaataaac agttccaggg cggcagagtc gaaattcatg ccgcgcgccg ccgtttcctt
    30481 attaatgagg ggcattaaac tgccttccca gcctgctttc gtgatgtccg gcttgttcaa
    30541 ttcttccgat gcggcgtatt gaagcaggaa tttgttaaag gaacggcgtt tgtcaaattc
    30601 ggaggcggaa ataagcagca ggacgtccgg ccccaggccg ctttccagca attgcctgaa
    30661 agaatccaga gccccttccg tggtggagga acgccccgtg acggagtctc ccagaaaatt
    30721 gcagttcttc atccagacta ctttgcgccc cggaaaaaac gggagggtcc gcagagcttc
    30781 cataacctgg cgtaccagct gggccgcttc atcggaatca ccgcaggccg cttccagaat
    30841 ttcatgggaa aattcgtttg tgccttccgt cagagcggca aagatgccgg aagccttttc
    30901 acgcaccatt ccttcatccg taccgaagac gagatgaatg gcctgttgtt gtaaagtgct
    30961 ggaacccata aaaccgtagc cgcatgatat cataaagtac gccttgttga aaggcgtatt
    31021 tgtcatttga aagttttttc atttgaagag aaagtactta aatagtatca atggaaccgg
    31081 aatgtcaaaa agcggctgaa gcttgacagg ctccgttttc aggcatctcc ttgtgatcaa
    31141 gccgcaaacc gtttgatcgt tattacggca gggcatttcc atgagatcga ccatatggac
    31201 acgggcttta cttgtcatca tgatgacggt tatgatgacc gtggtctccg ttgcctattg
    31261 gagatggaac agtcgtgtta cggagaagaa tgtttccttc cggtcggaag tgtttaccct
    31321 ggcccagcag cccgaattga aggaggagga tattcctgtt ttgcaccagc tcaatgagga
    31381 atattcacgc gttgcccggc tggtggaacg ggtattggtc agcatagaca ctacaggcgt
    31441 gacaagcgtt cctcagcctt ccgaggatgg tgatttcatg gttgaaaaga ggctgacggt
    31501 tcatggtttg ggttccggcg tcattgttac tgaagaaggg catattatta ccgcctacca
    31561 tgtcattcag aacaagcatg cgctgcgcgt tacgctgagc aatgggaaaa gcgtttccgt
    31621 acgcctggta ggggtggatc cggaactgga tattgccgtg ctgcaggtag aaaacccgat
    31681 gacgtttacc ccccttccgt tcggcaacag tgatgaaatt gcgcccggca tgattgttct
    31741 ggcatgcggc aatccctatg ggctgggcac gactgtttcc cgcggcatta tcagtgcccg
    31801 tgaacgcaag ctggtggatt ccggcattaa cttgatccag acggatgcca gtatcttccc
    31861 ggggaattcg ggaggccccc tcatcaacat ccgcggggaa atcatcggca tcaataaatc
    31921 ggtgcttccc aatgcggaga aaaattacgc cggcattggg tttgccattc cctccaatct
    31981 ggtgatgcac agctttgagc agatttgcaa acatggccgc cccatgaaag ggtatctggg
    32041 attggacatt gtgcgcaata ctcccccgct tcgcagcttc ctggattatc atgaggcggg
    32101 aggcgcagta atcaacatcg tgaagcatgg ttccccggcg gagcaggcag gccttcaaac
    32161 aggtgacgta atactgagtt ttaacgaagt ccccatccag acggatgagg aagtgaagga
    32221 acgcattgaa aatctttcca tcggcgataa gttccggctg aaagtctggc gcaaagggca
    32281 gaaaatgaac gtcaccctga aggtgggaga tagtatttta aaaaatgttc cttctccttg
    32341 ggaagacttc atggaaggag tgggaatgca tttgcgtgaa ctgactgcgg aagaacaatc
    32401 cataggtgct cgagggttgc tggtcattca agtgaatccg aagaaatccg tagggcagat
    32461 attgcaggcc ggcgacatgg tctttgccgt aaaccaccag agtgtcagca ccaaggaagc
    32521 cttggtcaaa gctcttaggg aagggccggc attgctgacc gtttcccgcg acgggcagca
    32581 attcaatgtg aaagtggatg cgcgccccgt ttctgaaaaa acattgcttc cccgcattcc
    32641 ggaggcgaca aaaggcaacg ccatcatacc gcacgagctt tgatccggcc tgagcggttc
    32701 ctgccggaac ctccgttagg aaaaacgttt tccggcatcg ccgtccgcag gcttgactgg
    32761 cagtcaaaag tgcttacaat gcatcacctt taagaaatta aattatgact cgtcatacta
    32821 tctccgtact tgttgaaaac aaatttggcg tgcttgcccg cattgccggt ttgtttagtg
    32881 gacggggcta caatattcat tcgttgaacg ttgcccctag ccaggacccg cgcttttcac
    32941 ggatgaccat cgtcgtacgc gaaaaggaag atgttctgga ccagattatc aagcatctgg
    33001 aaaaactggt caacacggtg gaagtagtag atttccgcaa tacggacaat gtgtaccggg
    33061 aaacggtatt gacccgcatt ggcgtagatt ccgccacgcg gcatgaagtc attgagttct
    33121 gccagatact gggcgcccac attgtggacg tcacgcggga cgccctgacc attgaagtga
    33181 cgggaggcga gtacaagata gaccgtttcc tgtccctcat tgaggattat gatgtgcaga
    33241 tgctgacccg gagcggccgt atcgcccttc ccaaaccctg ccagtaaaag aacctcccgg
    33301 ttttcttctt caaccctgca ggacattctt gcagggtttt tttctcatct tcaccatgaa
    33361 cattacttat ccggatctac ccatttcccg ccgccggaat gacattctgg cggcaatgcg
    33421 ggagcaccag gtcattgtcg tagtggggga gacgggatcc ggtaagacta cccagctccc
    33481 taaaatggcc atggagcttg ccggggaggc gcggggcaga gtagggtgta cccagcccag
    33541 aaggctggct gctgcttccg tttcccgccg agtggcggag gaattgaact gcgacctggg
    33601 aggtttggtg ggttaccagg tgcgctttga ggaaagagcc gggccggata cgcgcctgaa
    33661 gttcatgacg gatgggattc ttctggcgga aacgcagcat gatccggatc tgcgccaata
    33721 tcataccctg attttggatg aggcgcatga acgcagcctc aacattgact ttttgctcgg
    33781 ctatctgcgg cttttgctgg acaggcggag agacttgcgg ctggtcatca gttccgcgac
    33841 gctggatgcc ggcgggtttg ccgaattctt tggaggcgca cctataattc aggtagaagg
    33901 ccgcacttat ccagtggacc tccactatct gccgccacag catgacgatg aggaacttcc
    33961 ggctcatgtg gcgcgtgccg tggattggct ggatactctg gacgactgcg gggatgtgct
    34021 tgtcttcctt cccggagaac gcgaaatacg tgaggtggcg gaaaaactgg aaggccggaa
    34081 tctgagaaac acgcgcattc tgcctttgtt cgccaggctg ggcctggcgg accagcaaag
    34141 gatttttcat ccggagcagg gataccgccg catcgtgctg gctacaaatg tggcggaaac
    34201 gtccctgaca atccccggca tcatttatgt tatcgactcc ggcctggcgc gcgtcagccg
    34261 ctatagcccg gcacgccaag tgcagcggct tcagatagaa cccgtctcca aggccagcgc
    34321 ccgccagcgc gccggccgtt gcggccgtgt gtgcgaaggt gtgtgcatcc gcctgtacag
    34381 tgagaaggac tgggaggaca ggccggactt tacggacccg gaaatcaggc gcagcgcact
    34441 ggccggggcg ctgctccgga tgaaagacct gggattgccg gaaatgccgg agttcccgct
    34501 tccggatcct ccttcatcca aactggtgac ggaaggttac cgcaccttac gggagatcgg
    34561 cgctctggac aaagcccggc agctcacgcc cgtaggcaga aagctggcgc ggttgcccat
    34621 tgacccgcgg ctggcccgca tgctgctgga ggcgcaacat gaacaggccc tgccggaaat
    34681 gctggtgatt gtagccggcc tgggcatcat ggatccccgt gaacgccctg cggatgccgc
    34741 tcagaaagcg gaccaggccc acgcgcagtg gaaggaagaa gacagtgact tcctgtctct
    34801 gctcaaatta tggaaggctg catggcaatt ccgggaggga aggcgctggc gtaaaaacca
    34861 gcttcggaaa tggtgcggga aaaatttcct caatttcaac cgcatgatgg aatggttcaa
    34921 tttatgggag gacctttccc ggctggtcag ggaaacactg aaatgcagaa tttccccttt
    34981 ggaagaagaa acggagcggc aggcttcctt tgccatgatt caccggagca tcctggcggg
    35041 cgttccgcgc cagttcggcc tctgggatgc agacaaccgg gaatatcgcg gcgccggagg
    35101 gcgttccttc ggcatttttc ccggttccgg cctgttccgc cgtaaaaaaa ggagtgaatg
    35161 ggtaatgggc gtggagttgg tggacaccac gcgcctgtgg atgcgccgcg cctccatttt
    35221 ggaaccggag tgggtggagc tggtagcgcc ccatttgtgc gaatcacact attccggagc
    35281 gcggtgggac agggagcaag gggctgttta cgcagtggaa cgcgtcgtgt gcggcggctt
    35341 gcgcattatt gataacaggc gtgtgcacta tggccgtatc aatcccgccc atgcgcggga
    35401 aataatgata cgtgaaggcc tgttgggcgg tggtttccgc acgaaacccg cctgtatcag
    35461 gcatctggaa acactccggg aagaggttcg gcagatagag ctgaagttgc gccgtccgga
    35521 ccaggtctgg tgtgaggaag gagtttttga attctttaac aggcttgttc ccccggactg
    35581 ctgtacggcc agggcttttt tgcggtgggc cgccggtgtg gagaagaaaa acccggaagc
    35641 tctgcatgtt ccgccacagg aagccatgta cgaattctgg gggaaagacc tgctggatgg
    35701 ttttccggat gaaatcacct gtaaaggagc ggaatatgcc gtgtattacc agaatgatcc
    35761 cggcgcggaa gatgacggcg tgaccctggg cgtccacatc gaccagttgc cggatattcc
    35821 cgaatggttg ccggagtggg gggttcccgg ccatctggcc cagcgggcgg aatgcctgct
    35881 gcgttccctt cccaaggatt tgcgcgtctt tctccagcct atcagccaaa aggccgcttt
    35941 ctttgcggaa ctgaggcatg gcctggaacc ggacgggccg ctggcgggga aactggcgga
    36001 atttgtggaa gtggaaacgg gaaaattttg cgcgccctcc ttttttgaca tgaaccgtct
    36061 tcctgcggag ctggttacga aaatatgggt gtgcgatgat gaaggggaag agcttgccat
    36121 gggcacggat gttgcggaac tgaataaccg cctgggtaaa aaactttccc gccggttcca
    36181 ggaaacagcg gaggatatcg tgtccgtaac cggcatgaag gaatggagct gcggagattt
    36241 ggaatacacg gtggatgtgg cgggaaggcc gggttatgtg gctctggtgg atgaaggagc
    36301 atccgtgggc gtccgtgcct ttgaggatga actgagcgct gcggaatccc accgggaagg
    36361 atgcctgcgt ttcatgcgcc tgcgccagac ggaccagctt aaccatctcc gcagaaaatt
    36421 tccgttgaag ctggaaggaa agctctctct tcatatgctc ggacgggagc cgtctaccaa
    36481 tgcggaagac ctggtggacg ttgccgcgga gatagccatg ggccgtcctt tgccgcgcac
    36541 ggcggagcaa ttcgctgctg cggaaatgaa tctgcgccaa aacctttttg acgcggcgca
    36601 taacgtggcg gatatatggg aactggtggc gggtacggaa catgccgtca gggactttac
    36661 ggcgctgcaa aagggtgtgc gccatacgga gcgcatcacg gctgaccttc agcgccagct
    36721 tgactggttg ttacgccccc gtttcctgtg ggcgcatgga gcggaacgct tgccggacct
    36781 gaaacggtac atgcagggaa ttgcggagcg tatcagacgc atcggccagc aaccccttgc
    36841 cagggaattg gaacgcctgg acctctttga acgcgtgtat tttccctggc atcaggtttt
    36901 gaaggaacat gccggagacc tgcgctggat gcaatacggt tatttgctgg aagaataccg
    36961 cctggccgtt tttgctcccg ccatctccgt caagggccgc gtctccgaaa aacgtctggt
    37021 ggcttctttt gaggcattgt caagccagta aaaccaggac ggaggctgtt tgcacaggcg
    37081 gcttatccgg cgcctttttc tgaaatggag agggataccc tcagcctgct cgcggaggat
    37141 ggtttacaat cctttggata attgggcgaa ttcaatcaaa ttggtttctt tgccgaacag
    37201 gaccagaatg tcgtggtctt caaaaacgag atccggggaa ggggtgccaa tgacgggaat
    37261 gcgcggtatg gacaggattt cccggcgccc ctgcggagtc tgggcctccc cgcggcggat
    37321 ggtgagcagg ttgaggtggt gttccgtgcg caggcctaca tcccgcaggc gcttgcccac
    37381 ccagatgcgc ggcacggcta tttccacgat gctgtggttt tcgtccatgg gggagacgcg
    37441 caggaattcc ggggattcca actggctggc gagctgcttg gaagccacgc gggacaagtt
    37501 gatgagcgca tacacgttca tctggcttaa aatgtgttcg tgcaccggac tcatgacgcg
    37561 ggcgtagatg tagcggacgc ccattttctg aaggtgcgtg acgacgagaa ggttttcttc
    37621 aaaactgtct ccgatggcga caatgacggc ggactggtcg tccagttcca actcacgcaa
    37681 agcggccgga ttagtgtaat ccgtcaccac ggcataagtc accagatcct tgatttgctc
    37741 caattcttca tccgtggagg caacggctac tacttcaata ttacgggcgg aaagttctgt
    37801 gcagagttcc tgcccaaact ggcccaggcc gataacggtg tatctcataa tggtatattc
    37861 aggttaacgg gattttgacg gatggatagc ggtaatgcct cggagtaggg gtgccggcca
    37921 gcgcaatgag aaaagcgaag agccccacgc ggccggcgag catattgacg ataagaaggt
    37981 atttggaagg gccgcttaaa tctggagtaa tgccccgcga cacgcctacc gtagcaaaag
    38041 cgctgcagtt ttcaaagaag atatccagag aactcatgga gggctccagg gcgcaaatca
    38101 ggacggtaaa gcagccgatc caggcgcagg agaccaccac ggtaatcacg cagcgcgcca
    38161 cggtgcgctg gtcgatgcgg cggcggaaga attccacatg atccctgccg cggaggatgc
    38221 gcaggatttc cgcgcctgca atggcgcagg tagtggttct catgccgcct gccgtgccgc
    38281 cggggctgcc tcccaccatc atcagaccgc acataatgag gctggctgcg ggcagatagc
    38341 ggccgtagtc gctgatattg aatcccgccg tgcgtgccgt gacgctgtcg aaaaggcaaa
    38401 tccagaggtg ttcccccaac ggttccgcgg catggggaga ggaaacggca aagatggcca
    38461 gggcgcctcc cgccagcaaa atccccgtgg agatcatcac cagtttgaag taaggggtcc
    38521 actgcatgtg caggcggcgg tgtttggaaa aaaagcggtt tgctaggcgc gaattgattt
    38581 ccttgtaaat gccgaatccc aggccgccgc atacgatcag tgccatgatg accgcctgcc
    38641 cgctgtagca caggcggatg cccggctcca tcaaattcat cgggaaagtt gaaaaaccag
    38701 cattgcaaaa ggcggaaacg gagtggaaca gggaatgcca ccacaaaggc tcccccatca
    38761 ggttgatgtc cttcccttcc cannnnnnnn nnnnnnnnnn nggacggcgc cgcacagttc
    38821 gatgaacagc gtactgacca caatagtggt gataaaggag acgacggccc ccagattgtc
    38881 ttcatcaagc agatccgtca gcagcacgcg gtccctcagg gaaaaaccct ggcctgccac
    38941 catggctacg aagtaggcga acgtcatgat gccgaatccg ccaatctgga taagggcaag
    39001 cgtgaacatt tctcccagcg gggtgaaagt atgggcaaaa tccacggcgt tcagaccggt
    39061 gacgcatact gcgctggtgc tggtgaacag ggcatccgta aaactgagtg taatacccgg
    39121 atatgtggaa ttaggcagca gaagcaatcc ggtgcctgcc aggatgacta ctcccagaga
    39181 agctacaaac tgcatggcgg gggccataag aggccggcct tttttttgct tgcccaccag
    39241 ccattcaaaa atcctggtca gggacatcag cgtggggatg gcaatcagcc cggccgtaat
    39301 caccaggcgg gaaagctccg tgaacctcat gtatcccgct tcctgttgtt ccctggcaat
    39361 caggagaaaa atcatgaggc agctgatgat ttggaaaatc aggagcctca gggaagggcg
    39421 gttgatttcc ttccggaaat aggaacccag aatnnnnnnn nnncgtggca acggctgcaa
    39481 tcagccgcga ccactcatgt tccgcccgcg tcaggggcca gcccagctcc cagaccaccg
    39541 cccccagaag aatggcaatg cagacggctg caagtttggt gaatcccgag cgtgtagtgg
    39601 tcatgaatgg aaatgcctta tgcggactct accacctgga catggaaaga aaagctctta
    39661 ggccgtttta tgcatgcggg cgggaaaggg gcagatgccg cactgcgttt cttcatggcg
    39721 gtgcagggtc ggccggaaag tgagtggaat agtgatattg atgaatttca gtaaatttct
    39781 cttaccgtgg atatgcggga tggaaacgct gccgaagagg gccttggtca aaaagggaac
    39841 atgtttatca aaaggcatcc cgcggcaagc cgtattttag gcttttcctt tggcggcggt
    39901 gatggcttga tagggccgtg ctggctaaaa gaatcattcc gtgtctggac gtaacggacg
    39961 gcagggtcgt caaggggacc aatttcatta atctgagaga tgccggggat cctgtagaat
    40021 gtgccagggc gtatgatgcc cagcaggcgg atgaacttgt ttttctggat attaccgctt
    40081 cttccgatgg ccgtgccacc atggcggacg tggtgcgccg cacggcggca tgttgcttca
    40141 tgccgctgac ggtaggcggc ggcatccgtt ccgtaaagga catgcgcgaa atgcttctgg
    40201 cgggtgcgga caaggtttcc ctgaatacgg cggccatcaa caggccggag ctgattaatg
    40261 aaggtgcggc ggcttttggc agccagtgca tcgtcgtcgc tattgacgcc aagcgccagg
    40321 cttccggcaa gtggggcgtt tccacccatg gaggccggaa attcgtgggg ctggatgccg
    40381 tggaatgggc tgtggaggcg gaacgccgcg gagccgggga aatcttgttg accagcatgg
    40441 atgcggacgg agccaaaacg ggttatgata ttgaattgac ccgcgccgta agcagtgcgg
    40501 tgcgcattcc ggtaatcgcc agcgggggtg cgggtaattt ggatcacatg gtggatgtgc
    40561 tggtggaagg gaaggcggat gccgtgctgg cggcttccat tttccatttt ggggaataca
    40621 cggtgccgga agccaaggct tactttgctt ccagaggaat acccgtgcgt cctttggcgg
    40681 aatagcggaa acagcttttc ttttgagaga aaatgaaatt ccggtgcgta atatgcgtat
    40741 gcgctgtctt ttttttcctc tgtgttccgc tattgcctta acgtcttttg tccaggccca
    40801 gtccctggag aggcatcccc tgtatgaacc cgcccttgtt tccgtaggaa ctccgggtga
    40861 tgcaggtaat ttatttgccg gagccaaagt aaccgcttcc ggtcattacg gcagtgaccg
    40921 tccggaactg gccgtggacg ggcagacgaa taacgccggc aaatactggg gttgcgaggg
    40981 cattcccgtc tggctccaga tagatatggg aaagccccgg acgctttcct ccctgcatgt
    41041 atggccctat tgggagggag ggcgcattta caaatataaa atagaaggtt ccgaagacgg
    41101 aaagaactgg aaaatgttgg cggatcagtc ttccaacagc attgccgcca ctccggaagg
    41161 cgttcccttc aaattcaatc cgcagacggt ccgttacgtt aaaatcactt ttttgggcaa
    41221 tagcgccggc aatgagaagg gggggcatct ggtggaaatc aaagggtacg gacccgatgc
    41281 cgctctgagc ctgcaggctg cagcggtgaa ggattatgac cgtattcctt acaatggcgc
    41341 tcccgggcag gaaatgcttc aggatgccgt gcgtctgtcc ggctggagag gcgaacgcgc
    41401 tggcgggcag attgccgtct ggtcttccca ggcgcagccc cagttatcgg cctcctgcgc
    41461 cggggtaaaa aacgctgccg gacagattat ccctgtccgg actgccatga tacgctacac
    41521 tagggggggc aacaggctga tttcagatat catcggcagc gaaaacagct gcgatttgca
    41581 agccggaggc gtgcgtccgg tatgggtgga ggtcaatatc cccccgtctg ccaaacccgg
    41641 cgtgtacaaa ggaaaagtgg tagtttccgc agaaagcggc tctcccgtca gtgtgccggt
    41701 aattctggag gtggcgccgg aatccctccc tgctcccgcg cattggcaag ttcatctgga
    41761 cttgtggcag catccgcagg ccgtggcccg ctggcatgat gtggaaccgt ggtctccgga
    41821 gcatttcgca ctgatgaagc cggtgatgaa aaggctggcg gatgccgggc aaaaggccat
    41881 tacatgctcc ctgattgatg aagcctggaa tgcccagact tacgactggt tcccgcccat
    41941 gattgaatgg gtcaagggca aaaacggaac catgcgctgg aattacgcca attttgacaa
    42001 gtgggtttcc ttcatgatga acgaggtggg cgtcaagggg cagatatcct gctataccat
    42061 gattccctgg aacatgaaaa tccgttatct ggatgaggcg accggaaagt acaaatttct
    42121 ggatcttaag ccgaacgatc cctcctatga agctatctgg gggcctttcc tgacggattt
    42181 tcgcaagcac gtcaagagca agggctggct gggcaaaacc tgcatcgggc tggatgaacg
    42241 gccggacgct atggtcaggg cggccaagaa tgtactggac aagtatgccc cggaattcaa
    42301 aatcgtttcc gccgtcaatc ggcctacggc catgacccgg gacgtttatg acgtctctcc
    42361 tgtaattgac catgcaggca cggtcacggg cgatctgctg gcgcagcgca aaaaggaggg
    42421 aaaaaagacg acgttctatg tctgtgtcca tcccaaaaag cccaacacct tcactatttc
    42481 cccgctggcg gaggcggaat ggcttcccct ctttgccgcc gccaattatt tggacggctt
    42541 tttgagatgg gcttataatt cctggaaccg taatccgttt gaaaagacgg atttcgggaa
    42601 ctggcccgcg ggagactgct accttgttta tcccggcaat ctcagttccc tgcggtttga
    42661 aaaactccgg gacggactgg aggaatttga aaaggtcaat atcctgcgcg cccgcgccgc
    42721 aaaaaatcct aaggcgaaag ctgccgtagc ccgcatggat gaagagcttt ccaagctctt
    42781 taccgtggaa aaaagccgcg gggattccca tgaggaagac gtgcggaaag cccgcgaaat
    42841 tatccgtaaa acgacggaaa tttcccgctg acctttcttg cgggaaaaag ggaaaccgct
    42901 gtatttccat gtctttccgg aggttcggtt aagctccgga aaggggggag gatcagcggt
    42961 tttctctttg gtatttcgat ccccattccg ccatggaggc caggacgggg cgcagactgc
    43021 gccccagttc cgtaagagta tattccacgc gcggaggtac ttccgcatac acctttcgcg
    43081 taagcaggcc gtccttttcc atagaacgca ggttcgcggt cagaactttc tgcgtcacag
    43141 gcccaatgga tttgcggagt tctccaaacc gccgggtgcc ttcgagcaga tcgcggagga
    43201 tgagcacttt ccagcgggag gaaatcagtt ggagagtagt ttcaacaggg catgatgaat
    43261 tatagtaagt catttatttt aagcaatagt ttctttcagg aatccagggt actattttct
    43321 gcctacttta caaaaagaat gttgccgcct attttaataa gtgcctgccg ttacggcaag
    43381 catgaacaga taatgccatg aacaagatca tcattgccaa ttacagggag acggagcctc
    43441 aggttatcag ccgggagggt aattcctttg cggtaaagcc cgtcattcgg gaagcggatg
    43501 ccggaaaatg tgcggccagt ttcgtggaag tggagccggg gcggtacgcc tatggctacc
    43561 attatcatga agtgaatgaa gaggtatttt acatcatcag gggccatgcc gttgtgcgta
    43621 cgcctgaggg tgaaaagaat ttaaaagagg gggatgcgat tgcattcccc gccagcccgg
    43681 agggagcgca tgttatccgc aacggatccg ctacggagaa gctggtctat ctggatgtgg
    43741 gcactcgcct gacgcctgat gtggtgcact tcccggatac ggcatccgga atggtatatt
    43801 cctcctccgg tgtgcaccat tttcgggaat cttgaagcag catccctgtt ttaaagcctt
    43861 tttccggttt ttccaccggc gcggtccgca ggaaagcggg ccgcgctcct ctggggagga
    43921 aggtgtgggg aaacagtatt cccctggccg catgaaatgc ccctctttgc cttggacatg
    43981 gctggccgca aaggctgcgg ttcattgaag gccttcaaaa cctccatgcg gaaattaacc
    44041 cgtataaggt gatatattaa aacccggcct tgcagcaagg tgtgcgccct tttttgtgct
    44101 gcgggggaag gatgaattct ccggctttca ggaagggaaa gtactggcgc gggaagcctg
    44161 cttgcgtggc tgtatggcgg ggcggaaaag cagtcccctc cccgaaatat tcatggtttg
    44221 tttttcatga atatccggca gtaaaaaacg cccctgccgg ataagctgga caggggcgtt
    44281 gaaattaagc ggcctatacc ttattccgca ggtttgtcgg cacccatgcc ggcaatgctt
    44341 tggtacaggt tccagcggtg ccagacatcc ttctgggctt cttcaaggag tttttcgaat
    44401 ccttccttat tggtcttcgc cagaagtttg aagcggtttt cgctgagcag cacatcgcgc
    44461 agatcgccct tcgggccctt gccttccaca atcagcggat tcttgccctg gcgggccagg
    44521 tccggattat agcggtaaag caggactcgg ccggaatcca cccatgcctt ctgctgctgc
    44581 aggcccttgg ccatattgat gccgtgggag atgcagtggg agtaggcgat gatgagagcc
    44641 gggccgtcat aggcttccgc ttccacaaag gctttcagcg tgtgttcgtc cttggcgccg
    44701 agagcgacgg aggccacgta cacgttgccg taagtcatgg ccatgaggcc cagatccttc
    44761 ttcacgccgg gcttgccgtt cgtggcgaat ttggctactg cggcgcgcgg ggtggatttg
    44821 gagcactggc cgccggtgtt ggagtatact tccgtatcca tgaccagcac cttcacgttc
    44881 cggccgctgg ccagaatgtg atccaggccg ccgtagccga tgtcgtaggc ccagccgtcg
    44941 ccgcccagaa tccacacgct cttgcggaca agtttatccg caagggtcag gaggcgcgcg
    45001 tattccggct tgccggcaat tttcttcttg agttcttcaa tgttttcacg ctgctgggcg
    45061 atgtctgctt catccttctg cggattagcc aatatccggt ccaccagttc ctggcccacg
    45121 gcgccggcag cttcatgaag aagctcaatg gcgtgttcca tctgcttgtc cagggagacg
    45181 cggaagccaa ggccgaattc ggcgttgtct tcaaacaggg agttggccca ggcggggccg
    45241 cgcccttcag agtcatggga ccacggggtg gtgggcaggt tgccgccgta aatggaggag
    45301 caacccgtag cattggcgac caccaggcgg ttgccgaaga gctgggagag catttttaca
    45361 tacggggttt caccgcagcc ggcacaggcg ccggagaatt cgaacagcgg gcgcagcacc
    45421 tgcatggagc ggatgttgtc cgttcttacc ttggtgcggt ccacgtccgg aaggctcagg
    45481 aagaaattcc aattggcttc ttccttgtcg tgaatcttcc cggccgggac catggtcagg
    45541 gccttgtgcg tcgggtccgt cttgctcttg gccgggcaga catccgcgca gagcgtacag
    45601 cccgtacagt cgttcgggga aatctggatg acgaatttct ctcccttcca gtccggatgc
    45661 ttggcatcca ggcattcaaa cccttccgga gcgtttgcaa gagcatcagg agagaagaat
    45721 ttactgcgga taaccgcgtg cgggcagacg gcggtacact tgccgcactg gatgcagagg
    45781 gtcggatccc attccggcag atccagggcc agattgcgct tttcgtactg ggacgttccg
    45841 ttcgggaatg tgccgtccac gggcatttgg ctgaccggga tgctgtcgcc ttcaccgcac
    45901 atcatcttgc caannnnnnn nnnttttgta cgtagtccgg agcgtccggg gacatggcgg
    45961 gcggaatggc gtgtccgttt acagtttttc cggttgtgtc cacctgatgc aggttttcca
    46021 gcgtggcgtc cacggcggcg atgtttttgt ccaccacttc ctggcccttc ttggcgtagg
    46081 ttttggcaat ggccttcttg atgtagccga tggcttcatc cgcgggaatc acgctggcca
    46141 gcttgaagaa gcaggtctgc ataatcatat tgatgcggcc gcccatgccc gttttgcggg
    46201 ccacggcaaa agcgtcaatc gtgtagagct tgatgttctt gtccagaatc tgctgctgca
    46261 tgcgggcggg cagcgtgtcc cagagcttgt ccgccgggtg cgggctgttc atcaggaaag
    46321 tggcgccgtt ggcggcattc ttcaggaagt cgaacagatc cagcagggaa ggctggtgca
    46381 gggccaggaa gtttgccttg gtgatcaggt atgtggattt gatcggacgg ggaccgaagc
    46441 gcaggtggga aatggtggag gaccccgatt tcttggagtc atacacgaag tagccctgca
    46501 catacaggtt cgtatgctgg ccgatgatct tgatggcgtc cttattggcg cctacggtgc
    46561 cgtcggaacc gaggccgaag aacatggccc gcgtcacgtc gtccgcttcc gtggaataat
    46621 cttcatcata cgggaggctg gtgcccagta cgtcgtcgtt gatgccgatg gtgaaatgat
    46681 tcttgggagt gtccagagcc aggttgtcat agacgccctt gaccatggcg ggggtgaatt
    46741 ccttggagga gaggccgtag cgtccgccta ccaccttggg catgccgttg gtgaagggca
    46801 gcgtgccgtt ggcctgggcg tcgaagaggg cctggatgat gtcctggtgg agcggttctc
    46861 cctgggagcc gggttccttg gtgcggtcca acacggcgat tttcctgacc gtagcgggga
    46921 gggccttgac gagttcggcg gcggggaatg ggcggaacag gcgcaccttc agcacgccta
    46981 ccttggcgtt ttcccgggtc atcatggctt ctaccgtttc ttccacagct tcagcgccgg
    47041 atcccatcag gataatgacg cgttcggcgt ccggagcgcc ggtgtaatcc accagatggt
    47101 aggaacggcc ggtaagttcc gcgaatttgt ccatggcgtt ctggacaata gccggagtgg
    47161 cttcatagaa acggttgacc gtttcacgtc cttggaagta aacgtccggg ttctgggcag
    47221 tgccgcgcag aacgggagca tcaggggtca gggctcgttc ccggaaggaa tccagccatt
    47281 cctgcttaat catgccgttc agggtatcgt ccgtgatgtc tgcaatcttg ccgatttcgt
    47341 gggacgtgcg gaacccgtca aagaaattga tgaaaggaat gcgggattcc agtgtagcgg
    47401 cgtgggaaat agcggcgaag tccgcagctt cctgagtgga ggagccgcac agcatggccc
    47461 atccacagga gcgggcgctc atcacgtcgc tgtggtcgcc aaaaatggaa agggcctggt
    47521 aagccaggga ccgggcggct acgtggaaca cggcgggagt cagttcccct gcgattttga
    47581 acatgttcgg gatcatcagc aggagcccct gggatgcggt gaacgtagtt gcaagcgctc
    47641 cggtctggag ggcgccgtga acggtgcctg ccgcgccgcc ttcactctgc atttccacta
    47701 cggagggaac ggtgccccac aggttcttcc tgttgacagc ggcccagctt tcagctgatt
    47761 cacccatcgg agaagatggt gtaatggggt aaatggcaat gacttcagaa aaacgatagg
    47821 ctacggaggc tacggcttcg ttagcgtcaa tcgtgctgta agttacggtt gtctgatcac
    47881 tcataagtct gcacggatag tatataattg ataaataagg tgcgtcttaa cgggaacatt
    47941 tataacatgt ctggtgccaa aggtaaaagc aattatgcgt atgtggggat ttgccggcca
    48001 ttttttaata ataagaccag ttttgtttca attcctttga atatcgtgct ttatgtggtt
    48061 ctgtccaggc tttttgcaaa acggccggag tactggggca tgttccggca tccggcttcc
    48121 gaaaaagggg gggaggaacc ttcgggtcaa aaccggccgc caaaaatgtc ccctctgcct
    48181 gacaatgcgc tagccaaaac ggaagcaagc ctttagaatc cccgcactat gtccgaacaa
    48241 cagcaggcac atccgactac gacggaatcc gaacttattg ccgtgcgccg cgacaagctc
    48301 gccaaaatcc gtgaactggg aattgacccc tacggcgcaa gatttgacgt gaccaccact
    48361 cccgccggat tgaaagccga tttccaggaa gacaaacagg tagccgtcgc cggccgcctt
    48421 ctggctattc gcgacatggg caaatcccag tttttcgtca ttggggacgt acggggaaaa
    48481 atccaggggt tcctgcataa aaatgaggtg gatgaaacca cctggaagct ttggaagctc
    48541 ctggaccgcg gggactgggt cggcatcagg ggaaccacgt tcctgacccg taccggagaa
    48601 cctaccgtaa aggtatccgg actgaccatt ctttccaaaa gcctccgccc cctgccggac
    48661 aagtggcacg ggctggcaga caaggaggtg acctaccgca agcgccacct ggacctcatt
    48721 tccaatgagg aaagcgcttc cctgttcgtt acgcgctccc tcatgattgc ggaaatccgc
    48781 cgctttctcc aggaacgcgg ttatctggaa gtggaaaccc ccatgcttca ggatgtggcc
    48841 ggtggggccg ccgccaagcc gtttgaaacg taccataacg cgctggatat gccgctgacg
    48901 ctgcgcattg ccccggagct tttcctcaaa cgcctgatgg tgggcggctt cacgaaaatt
    48961 ttcgaactca accgcagctt ccgcaatgaa ggaattgacc gccgccacaa cccggaattc
    49021 accatgctgg aagcctattg cgcctgcggt gattttgaaa ccatggcgaa tatggtggag
    49081 gagctcatct gccatctggc ggaaaaattc tgcggcggcc ttcagattga ccacaaggac
    49141 gctgaaggca acgttcttta cacaatcgac ctcagccgtc cctggaaacg cgccgactac
    49201 caggacctga tccggggcgt ggcgggggaa gactggtttg acatttcccc cgaagcgcgc
    49261 cgcgcccgct gtgaagagct gggagtggaa atcagcccgg atatgaaaga tgtggatgtt
    49321 tcccagcagg tgtatgaaaa actggtggag gaaaagacca tgaacccctg ctttgttacc
    49381 cacgtggcca aggacttggt tcccctggcc aagctgaacc gggaaaatcc ggacgtagtg
    49441 gatgtatatg aactggtgat caacgggcag gaaatttccc ccggctattc ggaattgaac
    49501 gatcctgacg tgcaaaagga acgcctggag caccaggctg ccggggagac ccagcgtgtg
    49561 gactatgatt tcattgaaac gctggaatac ggaatgccct ccgcaggcgg catcggcatc
    49621 ggcatcgacc gcgtcgtcat gatgctgacg ggggcctctt ccatccgtga cgtgctgctc
    49681 ttcccgcagt tgaaacgtaa ggacagttaa tccgaaccac cggccgcatg gctccccgca
    49741 ccccggatat tctggactgc gcttccgccc aatggatcgg caaacgccgc gagcaggaag
    49801 acgtggttaa aagtcttccc gccgccggcg ggatgctggg catcgtgtgc gacggcatgg
    49861 gagggcatct tcgcggagat tgcgcctcct gtgtggtggc gcaggaattt gctgcggtat
    49921 ttgcggaaag cggcatcgag gatatcccgg aacgtttaac ggcggctttg catgctgcca
    49981 acagggcgtt ggccgccatt cagaaggaac cggaggaatc cggaaccaca ttgctggccg
    50041 tcttcatccg cgaccatttc ctgtggtgga tcagcgtggg agactccccc ctgtatttat
    50101 ggagcggagc ggacagcttt cgcatggacc gtctgaatga agaccattcc atgagaccga
    50161 ttgcggattg cctttaccgg aaggggaaaa tgtcacttca gggtgcgctt cggcagcgtt
    50221 gcattctccg ttccgccgtt atgggcggtc ccatggaact ggtggacatc agcgccatgc
    50281 ccctgctcct gcatccggga gatgcggtgc tggcatgctc ggatggcttg caggtttgga
    50341 gcgagctgtt gagagaannn nnnnnnntca aaaggccagg gattgttcca gcagggagct
    50401 ggtggggatc attatggaac aggtgaagga tatgctggag ccccagcagg ataatgcctc
    50461 cgtctgggct gccgtcatgc ggaaaccctg cgagggcggt aaataagctt attccggcga
    50521 aggaagtgcg ggagcatggt tctgggcggg cgggaaaatg ttcgcgctgt tcttattgcg
    50581 ccttaaacag ggcttttccg cagaccgggg cacggagagc cttgaaaatg aggatctgga
    50641 ggaggctgtc aggtatggtg ttcttttaca ggagcaaccc ctccggaaag aaccgggccg
    50701 gaggtgattt ccccattgtc atttgaccgg cgggggatta aaatgcactc cgcacaacat
    50761 gattgcctgg atagttttct attacctgat cggagcgatt ccgttcggct accttgccgg
    50821 cagaatcaga gggattgacc tgaggcagca gggttcccgc aacataggcg ctaccaatgc
    50881 gtggcgcgta ctgggctgga agtggggcct ttccgttttc attctggact tcttaaaggg
    50941 gttcatccct gtgttcggcg ccttgaactg gctgccgttc ctgacggggg atgcgggcgg
    51001 ctgggatttc aatgtcttgg tagttctctc ctgtttcgct gttatactcg gtcatactta
    51061 cacctgtttc ctgggattca ggggaggaaa aggcgtagcg acaaccgcgg gggttttatt
    51121 tgccctcaat ccggtggtgg cttgcgtagc tctggcttct tggctggtgg ttatgggaat
    51181 ctccggcatc gtgtccctgg ccagcctggt ggccgctgtg gtgatgatcg ccgccggatg
    51241 gtggatttat ccgctttgcc aggacgggga tgtctccccc cagatacttt acgtcgcctt
    51301 tttcacggtg gtaggcgcac tggtgattct caagcaccgt tccaatatcg caaggcttct
    51361 caacggaacg gagcactcct tctattccaa aaagtccaag taagatgatg aatcggttac
    51421 ataaaatagc agtcattgga gccggttcct ggggcacggc cctctccatg gttctggcga
    51481 cccgggaatg tgaagtcgtc ctgtggacgc cggaagaggc tcaggccagg cagcttgccg
    51541 aaacaagacg cagccccgtc ctttcgaaaa ctgcccttcc cctggccccc aatatccatc
    51601 ctacttgcga tctgggccag gtgaaggatg cggaactcat tgtagtagtg gtaccttccg
    51661 tggccatgcg ttccgtggcc cagcggctgc gcagcctgcc ggtgcagccg gatgccgtca
    51721 ttgtctcctg caccaagggc attgaacagg gaacccataa aagaatgacg gaaatccttc
    51781 aggaatatct gccctccaac cccataggcg tgctttccgg tcccaatcat gcggaagata
    51841 tctgcctggg ccttccgtcc gcctccctga ttggatttga agatccgaag tatgcggatt
    51901 gggtgcagca gatatttgca tccaaaacct tccgtgttta ttccgctacg gacattatcg
    51961 gcatgcagct gggcggaacc attaagaacg tctttgccat cggggccggc ttatgcgaag
    52021 ggctgaacct gggggataac gcccaggccg ctttgctgac ccgcggcctg gcggaaatga
    52081 cgcgcatcgg cgtggccagc ggaggccgca gggaaacctt catgggcctt tccggagtag
    52141 gcgatttgat tgttacctgc tattcccgcc actcccgcaa ccagacggtg gggcggagac
    52201 tggcggaagg gaagagcctc caggaaatcc tggataccct gggcatggtg gcggaagggg
    52261 tgcccaacac gctgtccgtc tatgaaattg cgcggaagct ggaggtgagg acgcccctga
    52321 ttgacgccgt ttattccgtt ctttatgaat ccaagccgcc gatggaagcg cttgcggaac
    52381 tcatgacgcg ggacccccgg ccggaattag attgattttt tgtttttttg agttgctttc
    52441 cctcgacaaa ttatac
//
LOCUS       DAWEMX010000004        50383 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_308_length_50383_cov_12.272214, whole genome shotgun sequence.
ACCESSION   DAWEMX010000004 DAWEMX010000000
VERSION     DAWEMX010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 50383)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 50383)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..50383
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_50383_cov_12.272214"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11007..11016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16109..16118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    44772..44781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    46983..46992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    48072..48081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11082 a        15567 c        13256 g        10428 t
ORIGIN      
        1 aacttatcag aaaaacgggg ccaataaaaa acgccatcct cctttacgga ggatggcgtc
       61 caaaatcctg aggcgtctcg gttagagcac ttccggagca agggaaacaa tcttcatgaa
      121 cttcttttca cggagttcac gagcgaccgg cccgaaaata cgggtgccgc gcgggttgtt
      181 gtccttgtcg atgatgacga cggcattgcc gtcaaaacga agcacggaac cgtcatcacg
      241 gcgaatcggg gcggcggtgc gcacgaccac ggccttcacc acggttcctt tcttcacgga
      301 agcggtggga atggattcgc ggatgtggca ggtaataatg tcgccaatgt gagcctgacg
      361 ggtgcgcttg ccgataacgc caatcatctt ggcggagcgg gcgccggtat tgtcggctac
      421 ctggactagg gattccatct ggatcatggt atcaaatctc ctttacttaa tggttgattc
      481 ctgattaatg ggtaagcact tccaccagtt cccagcactt cagcttggaa agcgggcggg
      541 tttcaacgat acgtacctta tcgcctacct tggccgtgga attttcgtca tgggcataga
      601 acttcttgct cttcttgacg atcttcttga acttggggtg cggaacgcgg gcaacgtatt
      661 ccacaacgat ggtcttgtcc atcttggtgg aagtcaccac gccgacgcgc gtcttgcgaa
      721 ggccgggctt cttggttgtt tcggtttgtt cggacattgg attctaaaag tgttagaata
      781 ttctgtaatt accggaaatc ctcaattaaa cctccccggt ttcaccctta atggtaaggg
      841 cgcgggcaag gtccttgcgg acggtgcgga ttctctggtt gttttccaac tggccggtgg
      901 cctgctggag gcggagattg aaaagttctt cacgaaggct gcgcaccaga gcggaaagct
      961 ccttggcgga catagcacga agttctttgg cggagttctt atcggacatg gtgatgagta
     1021 gctttagtgt tgaacgcctt gacgatagac gaagcgggtt gatacgccca gcttgttgga
     1081 agccagtcga agggcttcac gcgcctggga ttcggtcacg ccgcctactt caaacagaat
     1141 gttgccgggg cgaaccacgg ctacccagcc ttccacggcg cccttaccct tacccatacg
     1201 agtatccggg ggacgggacg taaaggattt ctgcgggaaa atgcggatga acaccttacc
     1261 tttacgtttc aggtaacggt tgatcgcaat acggcaggct tcaatctggt tgttggtgat
     1321 ccaaccgcgg tcgagcacct gaaggccgaa gtcaccaaaa gcaacatagg taccgctggt
     1381 ggcattgccg gaacggctgc cgcggtgcat cttgcggtgc ttcactctct tgggcattaa
     1441 aggcatatct tattcctcct tgtgttaagt gtgatgaaca aatgtggtag cggctttctt
     1501 aggcgcgcgg acgacggggt ccgcgagggc cggacggacg ggaattctgc tgggagacga
     1561 cttcatcgcg cttgttgacc cagcacttca caccgatgat gccgtacagg gtacgggctt
     1621 cggcaaagcc gtagtcaatc ggagtgcgga gcgtttgcaa cggcaccttg ccttcgcggt
     1681 accattcggc acgggcaatg tcagccccgc caagacggcc ggcgcagcgg atgcggatgc
     1741 cgtcggcccc gcgttccatg gcaacctgca cggcgcgctt catggcgcgg cggaaggaaa
     1801 cgcggcgttc cagctggacg gccacgtttt ctgcaataag ctgggcgtcc gtttcagggg
     1861 aacggatttc cacaatgtca atgttgacct gggtggaagc gccgcacagg ctctggaggt
     1921 actggcggat cttttcaatt tcctcaccct tgcggccaat gacaagcccc ggacgggcag
     1981 tgtggacggt gatgcggaca ctgttccagg cacgctcaat cacgatgctg gaaacggcgg
     2041 cggacatcaa ctgttccttg atgtacttgc gcatcaccaa gtcttcatgc agcttggtgg
     2101 cgtaatcctg gcccgtggcg taccacttgg agcgccagtc cttgttgacg gcgaggcgga
     2161 acccgattgg atttactttc tgacccatga tattagttca gctatatgtg tgttagattg
     2221 gtatggccta gccttcttga tcggccagga taacggtgat atgggatgta cgcttgcgga
     2281 tcatgttggc ggatccgcgg gcgcggggca tcgtgcggcg catcgtgggc ccttcatcga
     2341 ccatgacgga cttgaccacc agcgtatcaa cggacagttc cgcattattc tcggcgttgg
     2401 ccaacgcgga cttgagcgtc ttattcagca ggtaggcacc tttcttgggg gtgtaggaca
     2461 ggatgtcggt cgcctgggag acggacaagc cttggatttc ttgagcaaca tcgcgcatct
     2521 tcttggcgga gatgcgggcg tatttgtaaa cagctttcac ttccatgggt ttttcctcgt
     2581 agtgcaggtt attatgaaga tgatttagtc gttggttttt actgaggcag aatctccgac
     2641 gcgcaccgga tcggctggcg tctcaagttt ctgaacaagc gcgccacata cttccttgcg
     2701 gacgtccgtg actacggctt ctccgatggt ttgagctccc cgggttaacc gaaccggcat
     2761 cccgacttgc attcctgccg ttctgccggt attcagcacc accactccgg attcggaatc
     2821 aatgctgagc accttggctt ccgccagggt tcccgcgcca tctcgcacgg gctgttgcct
     2881 gtatcccaga acggattcca gcaggcgcag agaactctcc acagccgtcc tggcgttcgc
     2941 atcttcggat atggcttgtt tcatgtaggc aaggatggct ccggagagct tgacggatgc
     3001 ctgctccaac cgctggacgc ggtcattaaa agcttcgatg tccgccatcg cctgaatcag
     3061 gcgttcctcg ctgttaccta gcgcagcgcc ccccaaagct tccagcctgg aacggatgtc
     3121 caccagctgt gcggccgcct tgtcggcgtc gctgcgggcc tggacatagg atgccttgag
     3181 ggccgcattc tgcttttcga ggcgctcgat tctgcgcgtc agtgccgccg tatcctcttc
     3241 cgctccctgc aacgccccgc ccagccctac cagggtggta agagagtatg caaggatgga
     3301 agaagaattc gacatgttga ttgttaactt actgattact tcttaccgat accgccgtgc
     3361 tgtttgaaga cacgagtcgg ggcaaattca ccaagcttgt ggcctaccat gttttccgtg
     3421 acgtacacgg tggcaaagtt cttgccggcg tgcaccagga aagtcaggcc gacgaagtca
     3481 ggcgtaatca tggatgcgcg gctccaggtc ttgatcggct tgcggtcgcc ggattcaagc
     3541 tgagcgtcga tcttggcgag aagcttttgg ctgacaaaag ggcctttttt gagagaacgt
     3601 cccatgttat ataatcctag ttaaagttat aatatattga ttacttggca ttgcggcgct
     3661 gtacgatgac ggagtcgctg ggcttgcgga gacggcgggt cttctggccc ttgacgtggc
     3721 cccacggaga tttgaggtgc tggcgaccgc caccggactt ggacttgcct tcaccgccac
     3781 cgttcgggtg gtcgacgggg ttcatcgtca taccgcgcac ggtaggacga acgcccatcc
     3841 agcgggtacg gccggccttg ccggacattt cgttcatgtg ctgggtattg cctacctggc
     3901 cgatcgtgca gtagcaatct tcattgaaac ggcggatttc accggaaggc atcttgatca
     3961 gcgcgtaacc cgcttcacgg ttggaaacga tggcctgctg gccggcagcg cgggctacct
     4021 tgccgccgga acccggacga atttcaatgt tgtgaacgga agtacccaga ggaacgtttt
     4081 tcaagggcat ggcattgcct accttgggag caaccttctg gccgctttcc accttcatgc
     4141 ccacctgcag ccccgtgggg gccagaatgt aggatttttc accatccttg tactcaatca
     4201 gagcaatgcg gcaggtacgg ttgggatcgt attcgatagt aaggacagta gcgggcacgt
     4261 caaacttgtt gcgtttgaag tccaccaggc gatatttgcg cttatggcca ccgccaatgt
     4321 ggcgggtggt gatgcggcca ttgttattgc ggccgccgga tttcttgagg ggtctgcaga
     4381 gactcttttc cggggtagag gtggtgattt cgtcaaagga cggccacacc ttgtaacggt
     4441 tagagggagt aactggcttg aatgacttga gggacatgat agctttcctt cctaataggg
     4501 gctttaaacg aggtcaaggg tttcaccgtt ggcaagcttg acgtaagctt ttttccaatg
     4561 agcggtgcga cctgcatcag agcggcgctg gcgcttgagc ttgccgtcat agttggcggt
     4621 acgcacggat gcgaccttct tgccgaaagc aacttcgaca gcctttttga tttcgatttt
     4681 gttggcgtca cgatcaactt cgaagaccag ctcgccggta gtttcctgga gcatggtggc
     4741 cttttcgctg atgcgcacct tcttgatgac ttggtaaatg tctttcatgg cttcttaagc
     4801 agtacggctg gcgaggattt ctaaagcgtt gtcaacgagg atcactgcat cagcattcat
     4861 gagctgttca atgttcactt ccgcagcggt catcaggaga acttcctgaa cgttgcgggc
     4921 ggcacggtag gtggtttcgt caaaggaagc tgccacaatg agcaccttct tggcatccgt
     4981 caggtctttg acggctttga tgaaagattt ggtcttgccg tcagccacgg aaaattcgga
     5041 aacggtgctg accttggaag cggcaatcag gtcacccagt acgcggcgca gagccaggcg
     5101 tcgggtgctc ttgttcacct tcttgctgta gtcgcagggg cggggaccga agaccacgcc
     5161 gccgcccaca aagatgggag cgcgcttatc gccgtgacgg gcattaccgg tgcccttctg
     5221 gcggaagatc ttcttgttat tgccgctgac ttctgcgcgg gtcttggaat tggcggaacc
     5281 ggtgcggcgg ttcgcctggt aagccacgat caggtcatgc acagcctggg agcccttgtc
     5341 actgcctaca acctggatgt tggcggcggc ggcggcttct aatgtaaagg tatttgcgga
     5401 catagatagg ttttcctata atcaatgtga ggttaactaa agccttactt cttcttggcg
     5461 gggcgaatca cgaggtagga accgcgcgcg ccgggcacag cgccggagat aaggatgaca
     5521 ttatcttccg gacgaacggc gacaaccttc aggttctgca ccgtgcgctt ggcattgccc
     5581 atctgtccgg gcatcttctg gttcttccag acgcggccgg gggtggaaca accgcctacg
     5641 ccgccggtac ggcggtgcat catggaaccg tgggcggcgg gagagccgtg gaagttgtgg
     5701 cggcgcatcg ccccctggaa ccctttgcct ttggaagtgc cgatcacatc cacccactgt
     5761 ccggcggaaa agagatccac accgggatct tctgcgcctt cagcaggcaa ttcggatgct
     5821 tctacgcgga attccttgag gagtttggta ggctggatgc ccagtttctt gaagtggccg
     5881 gcctgaggct tggccacgcg gctttccttc tgcgcatcaa aagcaacctg gatagcattg
     5941 tagccatcct tgtcttccgt tttaatctga gcgaaggtgt tgcccttgac gtcaatgacg
     6001 gtcacgggaa ccatagcgcc ggattcctgg tcaaacagac gggtcatacc gacctttttt
     6061 ccgataagtc ctagagccat tgtagtgtgt ctttctgttt tttaattact tatccgggtg
     6121 ccaaactaga tgcggatcgt gatgtccacc ccggccggaa gattaagctt cttgagctca
     6181 tcaatcgtgc gcgcagtcgg gtcgacgatg tcgagcagac gcttgtgggt gcggatttca
     6241 aactgttcag ccgatttttt gttgacgtga acggaacggt tcacagaaaa cttttcaata
     6301 cgggtcggca acggaatcgg gccgtgaact ttggctccgg tgcgcttggc ggtttcaacg
     6361 atctcctggg aggagcggtc gatggcgcgg tagtcgaatg cgcggagtcg aatgcggatt
     6421 tttggacttt gcatggacgt gcggtctatt ggttatactt caaaggtttg tttatttagc
     6481 cttttcgctg acaatctggt caacgaggtt ctggggcacc tgttcaaagt gggagggttc
     6541 catggaatag gaagcacggc cactggaaag ggttcggatg gcggtggaat aaccgaacat
     6601 ttcggacaag gggaccatag ctttcaaaat gcaggcgttg gccttgtttt ccatgttgct
     6661 gatctggccg cggcggcggt tcaggtcgcc catgatgtcg ccctggtaat cggtcggcgt
     6721 ggaagcttca acggacatga tgggttccag catgatcggc ttggctttct tgaaagcgtc
     6781 cttcatggcg aagatggcag ccattttgaa agcattttca ttggagtcca cttcgtggta
     6841 ggaaccgtcc accacttcaa cgtgcacgtc caccaccgga taaccggcga cgacgcccgt
     6901 ggtcatggct tcattaaggc cggcataaac ggcgttcatg tattccttgg gaatggcgcc
     6961 gcctacgatc ttgttttcaa tggtaagccc cttgccgcgt tcgttcggct tcacatcgat
     7021 gaccacatgg ccgtactggc cgcggccacc ggactgcttg accagtttgc cgtcgccatg
     7081 ggcaggggca gtaatggttt cgcggtaggc aatctggggc ttgccgatat cggcttccac
     7141 cttgaattca cgcatcaggc ggtcaataat gatttccagg tgaagttcac ccatgccgga
     7201 aatcagggtc tgaccggttt cttcatcagt cttcacccgg aaagtcgggt cttcttcaga
     7261 cagacggccc aaggcattgg acattttttc ctggtcagcc ttggtcttgg gctctaccgc
     7321 catggaaata acggtttccg ggaaggtggg gggttccagg cagatatcat tgtccgggct
     7381 ggtgatggtg tcgcccgtgg tcacgttgcg cagccccacg atggcggcaa tgtcaccaga
     7441 gtacacggca tcaatatccg tatgctggtc agcctgaatc tgaatgatgc ggcccacgcg
     7501 ttccgtcttg cgggtgcggg ggttgtacac ggtgtcacct ttcttgagaa caccggagta
     7561 cacgcggatg aacaccagct tgccgacgaa tttgtcagcc cagagcttga acgccagcac
     7621 catgggcttg gcatcgtccg tagcggtgat ttcgaaggtc ttttccggat ccagcgtgct
     7681 ttccgcgtgg gcgggaacgg tttctaccgg agaggggagg taatccacca cggcgtcaag
     7741 caggaactgg acgcctttgt tcttgaaagc ggagccaccg gcgacaggca caaatttgtt
     7801 ggcgatggtg gcgcggcgga tagcttcctt gagctctttg ggcgtgaagg gttcttccat
     7861 gagcaccttt tcagcaagct catcgtccac gtcagccaca cggctcacga gttcatggta
     7921 ggccagttcg gcttcttcct tgagttcggc gggaatatct tcaatcgtgt aggtggagcc
     7981 caggcgatcg ttgtcggcgt aaatgacggc tttctggttc accacgtcaa tctggccgcg
     8041 gagctggtct tcagcgccga tggggatcag aatggcagcg gcattcgcgc ccagcttgtt
     8101 gtgaatatcg tccagcacgt tgttgaagtt ggcgccggta cggtccatct tgttgacgaa
     8161 gcacatgcgg ggcacgctgt acttggtggc ctgacgccac acggtttccg tctggggctg
     8221 cacgcctgcc acgccgcaga acaccacgat ggcgccgtca agcacgcgga gggaacgttc
     8281 cacttcagca gtgaagtcaa cgtgcccggg agtatcaata atgttcagct ggaaattttc
     8341 gccttcgaac actttgctga taccttcatt cttcacctgc ttccagttgg tggtaacagc
     8401 agcggaggta atggtgatac cgcgttcgcg ttcctgctcc atccagtcgg tcgtggtgga
     8461 accgtcgtgg gtttcaccaa tcttgtgaat catgccggtg tagaaaagga tacgctcgga
     8521 caacgtggtt ttgccggcgt caatgtgagc ggaaataccg atattgcggt aacgctcaag
     8581 gggagccttg cgattgggat tatttacgtg atcggacata gtgtctggaa agtatctgta
     8641 gtgatcgaaa gtgaatgatt cggctgacag tcgcagaagg aattaccagc ggaaatgggc
     8701 aaaggcgcgg ttggcctgag ccattttgtg aacgtcgtcg cgcttgcgaa cggaagagcc
     8761 ctgattgttg gcggcttcct tgatttcgtt ggcgagagcc ttgtgcatcg gcacaccctt
     8821 gcggttgcgg gcgtagttca caatccagcg catagccagg gattcggaac gggccggatc
     8881 cacttcaagc ggcacctggt aagtagcgcc accgacacgg cggctcttta cttccacacg
     8941 gggtttggca ttttcaatgg cgcgggtgat tacttccaga ggatcaatgc tgtccgtgcc
     9001 ttcgttagcc atgtcaatag cagcataaac aatacgttcc gcaagggagc gcttgccatc
     9061 cagcataacc ttgctgatca gtttgccaac aagagcgctg tcgtaacggg ggtcacgacg
     9121 ttcgattttt ctataaacgc gtttacggcg agccataata tatagtaggt aagatgtttg
     9181 gtttacttgg cagcagcctt cggacgcttg gcgccatact tggagcggcc ctggcgacgc
     9241 ttgtcaacac cgaggcagtc aagagcgccg cggacgatat ggtagcgaac acccggcaag
     9301 tctttcacac gaccgccacg aacaagcacg atggagtgtt cctgaaggtt atgaccttcg
     9361 ccgccaatgt aggcgatcac ttcttcacca ttggtaagac ggactttagc cactttacga
     9421 agagccgagt tcggcttttt gggcgtacgg gtcatcacct ggagacaaac accgcggcgt
     9481 tgcgggcagc tatgaagagc gcgtgatttg gacttctctt ccggagtaat acgtcccttg
     9541 cggacgagct gattaatggt cggcatgttt tcctgctttg gttgtggttc gtcggtctga
     9601 tccgatattc aaggcgaaca ttccttttta taagcaggaa gcgcgccttt tttccggagg
     9661 caaacccgat agaaaacgta tttcttgtgc ctttcggcac gtttggggac ggccaatata
     9721 acgtgatttt atatcatgac aagtctttta ttcatctttt cctaaatatt ttatccgttc
     9781 cattttcccc gctcctttca aaactccatt tcgggctccc ggggttcttt tttcctcccc
     9841 gtccagcagt tctccaccta tggaggatga aacaaaaaat cttcttcaca ggcaaaaaaa
     9901 ccatttcttc atgtccagca tgttaccgca gaaaaaaatg catgaagcgc cggataggca
     9961 cgaaaaacaa gctttccatc agaattcacg aaaaattttt tcataaaaac ggcagccggt
    10021 ctattattca ttgttcatcc gcctgctttc ccattttgcc ctgtccggaa agcatgacat
    10081 aaaaaaatcc gtttccggaa cgcatccgga aacggatttc atcaatgaac tcagcagccc
    10141 gctatttccc gggggtcacc agggaatgtc cggacatttc ggcaggctgt ttcaatccca
    10201 tcaagctcag caacgtggga gcgacatccg ccagaatgcc atcggagaga gttacctgat
    10261 ccttgtcgtc cgccacgtaa atgagatcca caaggttggt cgtgtgggct gtattcggag
    10321 aaccatcctc attgcgcatg tgttcggcat tgccatgatc cgccgtaata agcagcttgc
    10381 cgccaagttc caacaccttc ttcacacatt tttccaaggc gctgtccact gcttcgcagg
    10441 cggcgatgcc ggcttccaca aatccggtat ggccaaccat gtccccattg gcgaaattca
    10501 tgattaccac atcatacttg tccacagcgt ccacaaactt atccgccact tccgccgcgc
    10561 tcatctgggg cttcaggtca taagtagcca cttcccgggg ggaaggaaca aggatgcggt
    10621 cttctccggg gaactgggtc tccacacctc cgttaaagaa gaaagtaacg tgggcgtact
    10681 tttccgtttc cgctatgcgg agctgggttt tcccggcttc cgacacaatc tgcccgaaaa
    10741 tattgttcag ctgttcctgt tcaaaaacaa tgggagaggg atacttggca tcgtattccg
    10801 tcaacgtcac atagtgcact ttgggcgtta cttcacggtc aaatccgtca aattcggggt
    10861 aaaggaaagc gtcgctcatc tgccgggcgc ggtccgcacg gaaattaaag aagaacacca
    10921 catcgttatc cctcacgcgc tgttcgttgc cataggcaaa aatgccgggc ttcaggaact
    10981 catccgtctc ccccttcgca taacacnnnn nnnnnngctc cacatattcg gcaggggagc
    11041 aggagcactg ttcgccgcgc cccagaacga tggcatccca tgccagcttg ttgcggtccc
    11101 agcgcttatc ccggtccatc gcatagaaac ggccgacaac cgtggcaatt ttggcgccgt
    11161 acggcctgac ggcttcctcc agctggcgaa taaagccggc cccgctggtt ggcgagcagt
    11221 cacgtccatc cgtaatggca tggatgcaaa tatcgcggac acccgcttca taagcatatt
    11281 tgacgatgcc gatcaaatgg ttgatatggc tgtgcacgcc gccgtcactc accaggccaa
    11341 gcaggtgcag acgggaagaa gcggcctggg aaaaagcggt cttcagcacg gcgttttccc
    11401 ccatggaccc atctttgatg gcattatcca cccggcagag gtcctgaaag acaacgcggc
    11461 ccgcacccag gttcaagtgt cctacttcgg aattacccat ctggccgtcc ggaaggccca
    11521 cgtcctcccc ggaagcgccc agcaggctgt ggggatagtt ggcaaggagg taatcagtaa
    11581 agggggtatc agccagaaca gtggcgtcac cgtactcttt cgcgacatca ggccccaacg
    11641 gattgcggcc ccagccgtcg cgaataacaa gaacgacagg ctttttagaa gacataggct
    11701 ttatttacag gcatcccctc cttttgaaaa gcctgaaatc aggcattccc tctccgattc
    11761 cggaattgcc tgaggtttgc cgcccttccc cacacggacg actacatatc ttcccctcat
    11821 caccggacgc tcggaatgaa atacagcaaa tttccattcc agagaactgg ttcccaccgt
    11881 tcccaggctt aattctatgc tcagccaatc tcccagcccc gcagagctgg agtaatccgc
    11941 ctccacatgc acgcggggcc agccgcagtc tggggaaaga acctccaccc cccgggaacg
    12001 caggtactcg tgctccactt cttccaccaa acaaagcagc cgggagaaat gggccacccc
    12061 ggaagcatcc gtatcatgaa aagctacctg gcgcgttctg acaaagggca tgcagggaaa
    12121 atcttcaggc atgccgtcat cctgcctcct cccccggaac catgtccagc cgcaaatgcg
    12181 cgacaatatg tcacatcagc ttgatttgtg acatgccgct ctttatatat aagagcaagc
    12241 cgcacggcaa cggacccgcc gccatgaaaa aagacaccat tgacaagctg atcggccgca
    12301 ttgaccacat caaggaggag gatctccagc gcttttttgt caagctggcg gaacagcagg
    12361 gttttttcca gcaggtattt gaagccattc aggaaggcct gatcctgctg gataacaagg
    12421 gaaaaattct gttcgtcaac aaggctgccc ttaaactctt tgacaaggaa cgcgggcaaa
    12481 taaccccgga tgacttctgt attttcctgg gcagggactg ctcatgggac acgatccagc
    12541 agagccagac cgccgtctcc cgggacacgg aaatctttta cccggaacac agattcctga
    12601 acattttcat ttcccccatc ggcagcaaaa accagggcca cctggtcctg atacgggatg
    12661 aaacgccccg gcataaaaaa aacgcggaaa accttgaggc agaacgcctg aatgccttga
    12721 cgctgctggc cgcaggagtg gctcatgaaa taggcaaccc cctcaattcc ataggcctcc
    12781 atctccagct ccttgccagg aaggcaaagc agctgcctcc aaagtaccgg acggacatgg
    12841 aggaattgct gaaaacagcg gagagcgaaa ccacgcggct ggacgttatc ctgaagcaat
    12901 tcctccaggc catccgcccc accaggccca tccgggaacc ctacaacatt gaaaccatcc
    12961 tcatggaagt gctgaaactg ctggaaccgg aaatccagca gcgcggcatc caaatcaaca
    13021 cggacctcca gcccagtctt cccatcctca gcctggaccc cgtccaaatc aaacaggtct
    13081 tttacaacct gatcaagaat gcctaccagt ccatccctcc ggagggaggc accattctgc
    13141 tcaaaagcgg ttatacggat gacagcgtat tcgtcactgt ggcggatacc ggctgcggta
    13201 tttcaccgga ggtcatgggc agcatttatg aacctttcct gaccaccaag tccaccggct
    13261 ccggactggg gctgcttatc gttcgccgca tcgtgaagga gcacggcggg tccatcacgc
    13321 tggccagcca gccgggtcag ggaaccacca tcacggtctt cctgccgcgc gtggaacgca
    13381 ccatcaggct cctgccatcc tccattccct catgaagatg cctgttctgc tgattgtaga
    13441 tgatgaaaaa cccacgcgcg acgctctgcg catgggtttt caggatgatt atgaagtgta
    13501 caccgccgcc aatctctccc aggcttccgc cctcctgcag gaagaatctc cggacctggt
    13561 tctgacggat ctgagactgg gcggggaaag cggcatggac gtgctgaaag ccgctgcctc
    13621 cctgccccac cctccgcaaa gcatcatgat gactgcctac ggctccgtgg atgccgccgt
    13681 agcagctatg aaagagggag cgtacgactt cgtcaccaaa cctctgaatc tggacgccgt
    13741 ggagctggtg ctcaaacggg cgctccatac ccggaacctg gaaacggcca accaggaact
    13801 caccacccgc atccaggcgg actccggact gcaaaaactc ctgggcagat ccgccgccat
    13861 ggaacacgta ttctccatta ttcgtcaggt ggcccccagc aaaacgactg tgctgattga
    13921 gggggaaagc ggcaccggga aagagctcgt agcccaggcc atccattccc tttccggaag
    13981 gccggaaaat aaattcgtgg ctgtcaactg tgcggccctt tccccccaat tattggaaag
    14041 cgagttgttc ggccatgaaa aaggctcctt caccggagcc ggacaacgcc gcatcggccg
    14101 ctttgagcag gcggacggcg gcaccatctt tctggatgaa atcggggaaa tagacgccgg
    14161 aacacaagtc cggctgctgc gggtgctgtc tgaacggacc attgaacgcg tagggtccaa
    14221 tgcatccatt cccgtcaatg tgcgcgttat cgcggccacc aacaaatcat tgaaaaagct
    14281 cgtgcaggaa ggaaaattcc gggaagacct ctacttccgc ctcaatgtgg tgcatatcca
    14341 gatgcccccc ctccgggacc gccgtgaaga catccccctg cttgccaccg cctttctgaa
    14401 ggaattcgcc agggaaaaca acaaggaatt caaacccctc tcccgggacg cccttgaagc
    14461 cgtccggaat taccagtggc cgggaaacgt tcgtgaactc cgcacggcca tggaacatgg
    14521 cgtcgtcatg agcaattccg ccagcatagg cctccatcat cttcccccgc aactgcagga
    14581 tacttctccg gaagccgctc cacaggaacg gccggaggac aatgcaccca acacgcagaa
    14641 tgggcttgtc ccggccgggg ttttgaattt atccttgctg gaacggaatg ccatccagca
    14701 agcgctggcc cagtcaaacg gcaataaaac ggcagcagcc cacctgctgg gcatcagccg
    14761 gcgcactctt caacgcaaac tacaagacat cctccattca tgaatgagcc ctcctcggca
    14821 tcccgcaccc cttttatcat tatccttatc ctgctgctgg caatcacggg ggtgcagtac
    14881 tttttcaact ttaacgggct gggcactgat gccgccatgg accacgccca aatcgcccgc
    14941 aacgtagcca gggggcaggg gatgaccacc aactggctga ggcccatcca gatggtctcc
    15001 ggaagcacac gcgccggcct caaccctttt ctttcagacg cccagataca cgaacaggaa
    15061 gccatccggg ctggacaagg cgaaaccctc gtcgatccgg aaaaattcaa tccctatgcc
    15121 ctccgggaca cacgcaacgc tccgctgaac atcctgacgg aagcagctgt cttcaggatg
    15181 gccggcgtcc acaaatttga cctgtggagc atgacgggct catccatgat ttacctgcct
    15241 gaccggattg tggcgggcat ttcctgcatg ttcttcatcc tgtccgtcct gagctgctat
    15301 tacatcctcc tccggatgtt tgacgtcacc atcgcctgct tcacctgcct gacgatgata
    15361 ctcagcaatc ttttcctgca gtacgctacc agcggcctcc cgcagatgat gatgctcttt
    15421 ttctttgcct ggggcatcca ctttttgtac acggccctgc aaaacaagga ggaaaaccgc
    15481 gcattcctgt ggcctctcat cggcagctcc atctgttttt catgcgtctg ccttaccgga
    15541 tggatcgggc tgtggcccat ggccggtttt ctgatttttg caggcatccg gttcaaaccg
    15601 cacggcctgt actgcattcc gggtcttatc actctccttc ttttcctggc ctaccccatt
    15661 tacattaacc gctccctcag cggaggcttc ttcggaacgg cttattacac catcttcacg
    15721 gggctgaccg gcaatgaaga aatagcgatg aacgccctgg tttccgggag cattcccatc
    15781 gctgcccaaa aagtagtcac ttccgtcatc aacaacattc tgacacaggg tgacctgctc
    15841 tacgaaaacc tcggcaatct ccccctggcc atggtatttc tgctggccct cctgcataaa
    15901 ttcaaacgtc cggaagtcaa ccagtccaaa tggggcattt ttgccatgtg ggtccctgcc
    15961 gtcatcggca tggccattta tacctccaat aaaaccggca tttccttagg gcaaattcaa
    16021 atcctcttcg ctcccttttt cacggcctat ggcacggcat ttgttctcaa cctgatagcc
    16081 aggcattcca acaaagagct aatctccann nnnnnnnnct ccatccttcg gggaggcgtc
    16141 ctgctgctct cccttctcat cacttccctt ccgcttctgc tcaaccttcc gcacattgtg
    16201 cgggtgggca tcctgacggc cggccgcggc attccggcat ggccgcccta ctatcccctg
    16261 ggccttaacc gggatcttcg cacccagacg acggaaaagg atttcattct cacggaccag
    16321 ccgtccgccg tcgggtggta tgcagaccgg aaagccatcg gcatccccaa aatggtggaa
    16381 caattcatgg tgctggaacg cattttgaaa ttccacggcg gcaaggtggg cagcattctg
    16441 gtgactcctt cttccacgtc aaaaatgaac ctccgcacca tttcctcctc ctatggagaa
    16501 ttcactcccc tcgttctgga aggcacggtt ctgttccaga ccaaggataa aaaccccgtg
    16561 tacatctttg accacagccg ggcgcttgcc cccctggcgc agcgctttgg aacgcctgac
    16621 tcccgccaat tcattcaggg agcggaaatg attctttaca aagaccttca ggaaaccgcc
    16681 cctcaacctt aattcccata tggccaaaca acggaaaaac gctcccggat ttgaagaatc
    16741 cgtagcacgc ctggaagaaa tcatacgcct gacggaagcc cccgtcaccg agctggaaga
    16801 catgatagcc ctggtggaag aaggcaataa gctcatccgt cactgccgca gcatcctgca
    16861 tgatgcggaa ctgcgcattc aaactctcag caacccggaa accgttcagg ataaaacgga
    16921 tactgatgaa ccagacagca atgaattctc cctcacctga acttccggaa ctgctggcaa
    16981 gcatccgcag ccacgcagat ctgatgaaaa tccctgagtc ggaactgccc aggcttgcgg
    17041 aagaaatccg gaatactctc attcactccc tttccctcac cggaggccac ctgggcccca
    17101 acctgggcgt ggtagaactg agcattgccc ttcaccgcgt ctttgaaacg ccgcgggata
    17161 aaatcatctt tgatgtctcc caccaggcct atgtccataa aatgctgact gggcgcgctc
    17221 ccctgatcca caccatccgc cagcacgggg ggctttccgg atttgccaaa atgtccgaat
    17281 cccctcatga ctcctacgga gcggggcatg cgggcacggc gctttccgcc gccctgggca
    17341 tgtgcgcggc cagggatctg aaaggagaag actaccatgt ggtggccgta gccggggatg
    17401 cggcattcac ctgcggcacc actctggagg cgctgaacaa tatcagccag accaccaaac
    17461 ggtacatcac cattctgaat gacaacgaat gggccatcga caaaaacgta ggagcgctgg
    17521 caaaatactt caactccctg caaacatcgg aaacgttttc ctggttaagg gataaaacgg
    17581 cctccttcat tgaaaagctg ggcggcacgc aggccaggga ctttgctttc aagctggaag
    17641 gcaccaccaa aaaccttatc ttcccgtccc tgctgttcaa caaattcggg ctgcgctatt
    17701 tcggccccct ggacggacac gacattccca ccctgatacg cacccttgcc tacatcaagg
    17761 acctcaacga acccgttatt ctgcatgtgg tcacccagaa gggcaagggc taccagcccg
    17821 ctctggacaa ccccaccaaa ttccacggcc tgggctccta ttgtgtgagg gacggggaaa
    17881 cgcaggccgc ccccaccccc acattctccc atatcttcgg ctccacgctg gtggaaatgg
    17941 cgcgcgagga tgaatccatc acagccatca cggccgccat ggccagcggc accaagctgg
    18001 acttattcaa ggaagccttc cccaaacgct atttcgacgt agggatcgcg gaagagcacg
    18061 gcgccctctt cgcctgcggg ctggcggcgg agggcatgaa accctacatc gccatttact
    18121 ccactttcat gcagcgctgt attgacatga ttcaacatga tgccgcgctg caaaaactgc
    18181 ccgtgcgttt ctgcatggac cgcgccgggc tttccccgga cgacggcccc acacaccacg
    18241 ggctgtttga cattgccatg attcgcagta tcccgaacat tgtcttcatg cagcccaagg
    18301 atgaagccga attcgtccac atgctccgaa ccatgaacca ttaccagaac ggccccaccg
    18361 ttatccgcta tcccagagga tgcggcgccg gaacccccgt ccccgccaag gcggaaatcc
    18421 tccccatcgg caaggcggaa gtacttcaga caggacagga tgtaaccctc gtttccctgg
    18481 gcaccatgat cggcatcgcc cgggaaaccg cctcgctgct ggaagcccgc ggctacaccg
    18541 tcaccctgat caatgcccgt ttcatcaaac ctctggatga tgaatgcatc cgccgtcacg
    18601 cagcacgcag ccgggtggtc tgcaccttcg aagaccactc catcagcggc ggcttcaact
    18661 cagccgttct cgaatccctg gaaaccggaa atgtcaagac gcccgtggaa gccatcgcat
    18721 ggccggacca attcattgag catggttcgg aatccatcct cagaaaaaaa tatggactca
    18781 cggcggaagc cgcgctacaa aaaatcattc cccacctgaa ctcctgatct gtaactcctg
    18841 atctgtttat gaaaacacgc accttcctgc tggcagccct tgcgcttgcc cttcttcccg
    18901 tctccgcgcg gcaatccctg ccggcctccc cttcccctgc ctggattcag gaattcaaca
    18961 acctgccgga aacaaccaga aaaagctata tagcccaatt ccgcaaagcg gaacagctct
    19021 ttgcccagaa aagaatcatg gaatgtcttt tttccctcat ggagctggaa aaactttatg
    19081 ccggaaatcc gggcttatac aacctccgcg gcgcatgcta cattgaaatc cgcaatatcg
    19141 aaaaggccct ggaaaatttt gaaaaagccc agaaactgga tccctccaac ctcaccatcc
    19201 agttcaacct ggcggaagcg cattacgtca atcacgacta ttccagggct ctgaaagcgt
    19261 tcacggaact ccttctcccc ttcaaggaca atcccggcat gacgcccctg ctgcaattca
    19321 agcgttatat ctgcgcccgc aaactggata accagcctct tgccgcggaa ttggaaaagc
    19381 tgtacggccc gatggatgat acgccatact actactgcac ccaggccatc ctcaaactga
    19441 tggaagggga caaggaagcc gcccagggac agctcctttc cgccatccgc atctacggag
    19501 gcaccagcgc catccaggcc ttcacggacg ccatgacgga ggctggcatc cttccctccc
    19561 cctatgggca aaccgtccca accgaacagc cggtaaaaac cggactggaa aaacgccgtt
    19621 aatgaatgcg gaagcgacgg tcctgaagct ttatcccctg ggagaaaacg gcctgattgc
    19681 cgtctggtgc acggaagaag gccttatcag gacggcggca aaaagcgcca gaaaacccgg
    19741 cagccccttt gccgggcgtc tggacatttt ctatcagtgc cgcatgcagt ggacgcaggc
    19801 aaagaagggg gatttgcaca ccctgacctc cgcagacctg ctttcaccgc gcctggccct
    19861 ccggaaaagc tacctccggc tcagtgcggc aggctatttc gcgcgcctgt ttctccaaat
    19921 gctggaaccg gacacgccca tcccggaatt ttacgacttg ctgcagaggg cctacaccta
    19981 cctggaaaac aatgatccca cgctacgcgc cgtcctgcac ttcgaacagg aactcgcccg
    20041 gctgcatgga atagcccacc caggcatccc ggcgcacgtc attctgaaat cccacttcgg
    20101 caagcttccc ccacagcggg aaaaactgct cggaagcctg gaacaaaaag aaagctgaac
    20161 aacatgccta tcctgctttc atcctcagca ggggatgttc cccgccccgc tcaacacgtc
    20221 cttcctccgg gacgggatgc gcattgttcc gtgcgcgaga ttgacttgtc cgcagccttc
    20281 gggcagaata tccggcacgg ttttactgca tgaagatcct tgacagatac attgcccgcc
    20341 agctgctggg tgtcacggcc ctgggggtaa tggccctgag cgccctgctc cttctgggca
    20401 acctgttcaa ggaactgcgt cccctcctgg tggaaaacaa ggctcccatc agcatcgttc
    20461 tggaattcat cttccaggtc atcccctttt cgctgatgtt ctccattccg tgggggttcc
    20521 tgacggccgt tcttctggtg tacggccgtc tggcctcgga caacgaactc acctccatgc
    20581 gcatgtccgg catgagcctg tggcgtctga gcgcccccgc catcgccatc ggcatcgccc
    20641 tttccggcct ttgctactgg attaacatag acatcgcacc gcgcgccaag caatccattt
    20701 ccgagttgct catcaaagcc gcatccatca acccgaaggg gcttctcaat gaaggccagg
    20761 ccattaccaa attcgacaac ctggaaatct acatcgacaa acgcgtggac gacgtcatcc
    20821 acggcatgca tatctaccag aaagcggacg acaaatcccc gtctgtggcc atgcactcgg
    20881 aacgcgtgac catggatttc tcccccgaga aaaaaatcct caccctgcat ctcatcaatc
    20941 ccctcatcac cacgcaggaa gaaggttcca tctcccaaag cgtgaccatg gacgagatgc
    21001 ctctgagcat caacctggac aaatcccgct cccggcgcat caaggccaac cgcttcacca
    21061 accgggaaat ccgggaagct ctggacacgc ccggctatct ggataaaaag cagaccacgg
    21121 aattcgccac ggaactgccc aggcgagcct ccttctccct ggcctgcatc gtctttgccc
    21181 tcatcggcgt gccactggcc atcaacaccc gcagaaaaga cacctccaca ggatttgccc
    21241 tgggcatcct gatcgcctca ctctactttc tggccctgat ctttgcagac ttgtcccgga
    21301 aaaacgacac gatgctcccc tatattctgc tgtggcttcc caacatcatc acggttgccg
    21361 tggcgttgca ccttcacaaa cgggccaggc acaagggata agcccccggc ggaagccctt
    21421 cccgctggcg tggaattagc tttcacagca tgggagcttc gggtaacatg atgtgcatgc
    21481 agcacgtccc caccatcgcc atcgtaggca ggcccaatgt cgggaaatcc gccattttca
    21541 acagaatggc aggcaggcgc atcgccatcg tgcatgatga acccggcgtc acccgcgacc
    21601 gcctttccgc cccctgcaag attacggacc gcgcctgtaa aatcatggat acgggcggta
    21661 tcggcgcgcg cctgagcgac ggctttgcag aacaggtaga ggcagaggcg gacattgcca
    21721 taaaaacggc ggacctcatc ctcttcgtgc tggactgccg ggatcacctt acacctatcg
    21781 accagagcat cgcggaccac ctccgtaaat cggacattcc cgtcatcctc cttcttaaca
    21841 aagccgacca cgaaaaacag gatcttaatc tgggcgaatt tgccggactg ggttttgacg
    21901 accatatctt tctttccgcc gcccacggca gaggcttttc cgaattagcc tcccggctgg
    21961 acggttttct caagcagaaa ggggctccgc ttaaagaaga gctggaggaa gaaccggaaa
    22021 acggagagga aacggccctt cccatcaagg tagccgtagt gggacgcccc aacgcaggca
    22081 aatcctccct ggtcaacacc atcctccggg acaggcgcac catcgtctcc aatgtggcgg
    22141 gcactacccg tgacgccatt gacatcccct acctgcacga cagccagccc tacgtgctga
    22201 tcgacaccgc cggcatgcgc ccccgctccc gtcgggacac ctccgtagaa gttttctctg
    22261 ccatgcgcag tgaaaaagcc atccgccggg cggatatctg cctgctggtc attgacattg
    22321 cggcgggcat cacgcagcag gaccgccgca ttgccggcat cattgcggag gaaggaaaac
    22381 cctgtatcat catcgtcaac aaattcgacc tcttccatcc gaatgcctcc cgtaaggacc
    22441 gcatggcgga agtggaggaa caggtgcgca gggaactctt cttcatcagc tacgcgcctt
    22501 tcatcgccac ctccgccaaa aaagcggaag gcgtggaaat catcttcaaa gtcatcacgc
    22561 gcatccgccg ggaatcccat aacctgccca ctaccggcca gctcaaccgt ctgatccagc
    22621 ttgcccagca gatgaatccc cccggcgccg ccagcggctc tgccagaagg ttgaaaatat
    22681 attacgccac cacagcggtg gaccccaagt acaatactat tcccgtcccg cgctacgttc
    22741 ttttcgtcaa tgacaaaagc ctgctcacgg acagctattc ccagtacctg cgcaacaaaa
    22801 taagggaagc ctaccccgct cccggcattc cggtcatctt ctccgcccgt tcacgcgtgc
    22861 gcaacgactg atcatccctc cttctccgca tgctctccgt caggaacctc agcgcctcct
    22921 tccacaccag ggccggcatt gtccgggcgg taaggaacgt gtcctttgat gtagccccgg
    22981 gggaaacgct tggcatcgtg ggtgaatccg gatcaggaaa atccgtcacc tgctactcca
    23041 tgatggggct cattccgatg cctccgggcc gcattgaaag cggttccgcc atgctggacg
    23101 gcacggatct gctccgttgc ccggaaaagg aactccgggc catccgcggc aaacgcatct
    23161 ccatgatctt ccaggacccc atgacctccc tcaacccgta cctgaccatt ggggagcagg
    23221 tggcggaacc ccttgtcatt cacgaagggg ccggcaaaaa ggaagcgcgg gcacgcgcgc
    23281 tggaacagct tgccctggca ggcataccgg atgcggaaca gcgcatggac gcatatcccc
    23341 accaattctc cggcggcatg cgccagcgcg tgatgattgc catggccctc atcaccaggc
    23401 cggaaatact catcgcggat gaacccacca ccgcccttga cgtcactgtc cagaagcaag
    23461 tgctggatct catcagaaaa cttcagcagg acatgggcac ctccgtcatc ctcatcacac
    23521 atgacctcgg cgtggtgcgc caatacgcgg accgcatcaa tgtcatgtat gcaggccgca
    23581 tcgtggaaag cgccccggcg aaggaacttc tggaacatcc ccggcatgcc tacaccagag
    23641 ccctgatgaa atccattccc gggctgcatg cgaaaggcgc ccccctctat accattcccg
    23701 gactgcctcc caacatgact caggagccat gcggatgcag cttccgcccc aggaacaccc
    23761 tcggaaaccc ggcactctgc ctcacggacc gggaaccgga gctggtggaa atatctccgg
    23821 ggcactcagt ccagaattgt ccgggctgcc tggcgtagct tcctttcttt cctgcctgtt
    23881 tgcacgcaga taatgccgcg cacaggaaaa atggaaaaca aagcgtcaat aacggtggtt
    23941 accccatcgc tctccagaga aaaacaacag gtttggcaat caagagaaaa aactttcttc
    24001 attcgcccgc ccacgttaag gaaatctcta aaggcctgcc ccctgccatg acattttaac
    24061 gtgctcagca cctgttggaa ccacgccacg gaaaatcctt ttaacagaat tgatttccgc
    24121 acaattcttc cagaataccg taaaaacggc ccacatgcac cccgtccacc agcacatggt
    24181 tcgcctgaat ggccacgggc atcaccatcc tgccggaagc atccgcctcc atcttccccc
    24241 aatggatgag gggaaagtat tgtccccgaa acactctgta ggcactggcc acctgtgtga
    24301 atggaatcca cggcaggcag ctgaatacgg cgatatccgc cctatggcac ggattcgcct
    24361 gggcgggagc aagtttggca gcctgcacaa gaggaacggc atgtttcagg aaatcagaca
    24421 actccatccg gtattccact aaaatctgag tgaattctcc atccgcagcc agcaccggaa
    24481 ccaaggcatc cacggactca tactcccata catccccttc ctccacccgc tggcgcagct
    24541 ggggcaccct gttcaacgcc ttcagcagca caaacaggca caaaatgaaa aaggaacccc
    24601 cctcccgttt cgcataacgg tacagggcat ccacccggac cggggacgta atggaaaaca
    24661 tggggcagtc aaacgtctgg aaatactcat aatgagacct tcgaggccag gaagagattt
    24721 ctattttctt tttcatgcac ttattttcta ttcagggaag gctccccgta cacgtttttg
    24781 cgtttttgct ccagaagcaa aatactgaac aggacgggag cagtcaaggt ggcgatgccc
    24841 gtaaatatcc cccccacagg atgccagcaa aaagacatta ccagaagctt tccctcaaaa
    24901 aagcaaatca ggagggcgat ccacaccggc caagttatcg ccagccagcc cccatccagt
    24961 ccggcatcat gaaaacgcct cacccccagc gaaatgaaac aaaataaaaa gaaaaacagc
    25021 agcaacagtc ccagcaaaaa agcaaaaagc gttccgaaag gaaggaattc aaaccaaacg
    25081 ctggctccac aaaaacacgc cagccatacg ggaggggcaa acaccagaag cgccccatag
    25141 gcaacaaccg cctcccagcg cgaagaacgc ccccgcatcg ccaacaggga aaacagccaa
    25201 ttaaaatgag gcttcctgcc gcacctccat actttgccag gtttcatcca ctcctcagtt
    25261 ccggcatctt gttccttgaa agactgccat tccggcggag ttccggaaac atcccctacc
    25321 catatccagt cctccgaatg ttcttcccgg accatcagac ccagcggcaa tccggcatag
    25381 catatttccc gaatcaaaaa atcgacatcg accggtccat gcaattcccc atccggcgta
    25441 tggtaataat aacgtttcac aacagcttct ttccttccat tattccccgg ctctaccgga
    25501 aattccagcc cggctgttcc cagcgccaaa gcattgatta atgcttctat ttattcaatt
    25561 atcaactatg ttgcaacaaa gaacatcctg aattccccct acccctcgcg aacggaaagc
    25621 aaatccgtta cggacgcatg cttcatgcgc cacgcaggca gaaggcacgc caaggatgag
    25681 agggaaagca ccaaaagcac agcacaggcc agatgcgccc agggcatcgt gacaggagga
    25741 gtaacgacgc cgaagtgata gccgtattcc aaaatctgga tggagcacca ggcgcccagg
    25801 actcccagca caagactcat gacaacggag cacagggaaa ccatcagcgt ttccgcccag
    25861 agcatccgca ttaccatgcc gcccggcacg cccaccgcac gcatcaatcc aaattcccgg
    25921 cggcgggact gcacggaagc caggacggtg ttaagtacgg ccagaacggc gatcaccatc
    25981 atgatgatgg gaagcttgct catcgtaaaa atcacatcgt ccccgcggct ggtaacgctt
    26041 cctcccaggc tttcccgggt cagggccttc accagaggct tggcgccttc ccctccgttt
    26101 tcctgctccc gcaccttcat cagggcgtat tcccccaggt cgttctgaag ttccacgttg
    26161 ctgatccccg gagcagtatc cccccagata aactggaatc cctgatgagc gtattcctct
    26221 ttgagccagc gttcatcggc aatcgccaaa gcggcaacaa aacccccgcg gcgcacgcgc
    26281 attccgctgg tcttggtcaa ccaatgccag ccggggaagg aaactattcc cgccaccttc
    26341 cagggttctc cccgtacggc gccccccctg ccccggccgc gaataccggc agagggctca
    26401 tttccggaac ggagttcccg ggaagacgga ttaaccagca tcaagtcatc cccgaccttc
    26461 aaccccaccg tccgtgcaaa cgtgtccgga atcaggagag aacgggtgga ttccagcatg
    26521 gcataagcct cctgcggatt tccggaaaca aacacggggt tgaacagcgg atggctcccc
    26581 ccggccatct ccgccacggg aatgccggcc agtacgatgg aacggttgcg catcccggaa
    26641 aaaccggggc ttttcatctg tgccggagca atatccggtt catccacgta gatgggatac
    26701 attcgggaat tccgcaggct ggggcgcgcc atcagctccg gaacagccgc agatttgaac
    26761 tccgtatgca ggaaggaaac cagggttccg ggtgttgacg tatccgggga aaaaggaacc
    26821 agcatggaat agccccatgt ctgcaccccc acaaaaagag agaggcccac agacatggat
    26881 acggcagttc ccacggaacg gctgaggttg cggctgagct gcattttcag gaaagaatgc
    26941 ggaacgtgca gcagaaatcc ggtaatccat gccccggccc actccgtcac gcgcacgaag
    27001 gtgggggcca gaaacagcgc accggctacc aggccggggt aacccagcca gaaaaatatc
    27061 catttgcgcg tttctacctc caggcccggc aacagcaggg ccgcaggctg aagacacacg
    27121 catgcgaggc ccgccacgac agaccacaca ggaacacggc tcaccttccc gatgaatccg
    27181 gaggaaggaa cggccgcttc cagcggagac tggcgggaag cgcgccacgc aggaatgatg
    27241 gaagccagca aggccccgcc cacagcgcat acggcggcgg ttaatactgt ggaccaggtc
    27301 agcacgggta cagaggcaga gccttcctcc agcaggtaca ccaggaaaaa gcctgcggcc
    27361 aaccctccca gcaatgccgg aatgcacaga aaaatcccct ctcctgcaat cagcagtgca
    27421 atttgcatgc gccccagccc cagagcacgc atcagggcca gtctgcgggc acgttcgctg
    27481 acgccaatgc tcaacgttgt aaaaataatg aaaatacagg aaaacaggac aagccagacg
    27541 gacatttccg cgctatcctt ctgtgaacgg acggaccgat cgctggaaag ccgccggatg
    27601 atggaatccg tatccgccac cgcagcggaa gcctggacca gctcctgccg gaaattctca
    27661 gcaaattcct ttttgcccac cccttccttg agctgaacgt aaatcaaatt gggggcaaaa
    27721 ggctgccccg taattttctc gcataccttc acgggcacaa aaagggagga aaaagccggg
    27781 ccggacatgc cgcccggccc cataatgacg ccgggggtgg ccttggcctg cttgacgatg
    27841 cccacaatct ttacgtcgta cacatgcgtt cccacgcgta cattcatgac agtccccact
    27901 cccgcgctga aatatttggc gcttccgctg cccagcaccc cctccatggc tgaggaggaa
    27961 gccatatccg gccaaattcc ctctttcaat tcataaggac actccacagc atggtttccc
    28021 accaggatgg ggctctgggg aggaattccc atgcggtcgc gcgtctgctc gtcaaagctg
    28081 ccccgctcat ttccgcagcc gatttggagg cgcggcacct ggcacgcggc gttcaccgtt
    28141 tccacaaggg gagaggcggc cagacgggcg ggcagctccg ggtcacggaa ctgcgggtac
    28201 tgtccgggag gaaggggccc tttgggagac tccggcacca ggcagaggtc ataattcccc
    28261 atataggcct ccgcatcatt gtcaaactcc ctgaccagcg tatcatagct ccccatcatc
    28321 catacaatga ggctgacgga gacaaggatg gcgaacacgc tgacagccat ccggccgggg
    28381 ttggcgcgca gatccctcca aatcagtttg aacatcaacg tcataaggcg tcattcagcg
    28441 tacgttcata gaaggaggac aattccgcag gaccggcaaa ttccgctgtg gatctgtcgt
    28501 ccacaatgct gccgtccttg agtacgatta cacgatccgc ccacatggct acgtccggcg
    28561 catgcgtcac caggacaaaa gcacaacctt gcgcacgatg caaatcccgg aacaaatctc
    28621 ccataagccg ggtattttca gaatccagat tgcccgtagg ctcatccgcc agcaccagag
    28681 cgggaccgga gaccaatgca cgggcaatcg ctacgcgctg ctgctccccg ccgctgagcg
    28741 tatccggcat ggcatgccga cggtgagaca gtccaacctt ttcaatcatg gcgtccaggg
    28801 cggcggaagc caccttcctg cctcccgcca gggacggcaa cagaatgttt tcctccacgt
    28861 tcaaggtgcc caccaggtta aacatctgga aaatgaaccc tacacggtcc cgccggtaca
    28921 ccgtcaggga ggcatccgac atggaaccga gatccatccc atccaccgtc accgtgccgg
    28981 aatccggagc cagcagccct cccagcacgt tcagcagagt actttttccg gagccgcttg
    29041 cgcccatcac ggcgacgaac tctccccggt tcacggtaag gctgatatct ttcagcacag
    29101 ttatactccc ggaaccggat ttgaaaccgc gcttgagatg gctgacggaa atgacggggg
    29161 aactcatggc ggaaacgcaa ggttaaaacg tggtcttcag gcctacttca aacgtgcgcg
    29221 gctctcccac cgtgcactgc acggcatggc gggtggactg cacgtacttt ttatcgaaga
    29281 tattatacac agccagcctc agcgtggcat ctttcagcca tttggattcc ggcaggggga
    29341 tttccaccgt aaagtcgaat acgctatagg aggggatggt gtagttgtcc gcaatttttg
    29401 cgccccggaa cgtagcgtag taggaatcct tgcagcggta gcccaagccc agcaccgtac
    29461 cgttcagcaa ccctccgtca atgcggtact tctgccagag ggccagagca ttcggagcaa
    29521 tcgtgggata cacttccccg gtggtccggt tcttggtgcg ggtgtaggtg taggaaagat
    29581 aggaactcca attcttggtt atttccccgt tcagggaaag ttccacgcct tccgccctct
    29641 tggagccgtc cgaatagtac cggttggtat atccgtctat ggcaacaggc gtattattct
    29701 ggatgatgtc aaaccaggaa gcggaaaacc acaccttatc aaccgggctg acccggaacc
    29761 cgaattccat ctggtccgta cgccaggaat cggtcaattc cttcccgttt tcatccagat
    29821 acccgaagtt gggggcggag gtacgggctg cgttggcgaa caacgctacg cgttcgccga
    29881 acatgcgggt aattccgaag cgggggctcc acgcaaacgc ataattattg tccaggctga
    29941 aatgcgcgtc accgcgaacg ccggccagaa aacgccactg tccataactg aggacatcct
    30001 gcagcagaaa accagccctc tgcaccacgg tatccgtcgc attggcgccg gaataatcgc
    30061 gccctgcagg gaaaaaagga ggaggattgt acagggaaaa atagccgttc gtatttgcgg
    30121 tggcgtttgt caccaggctg gaaccgtctc cataagtgct gctccccgtg taagaaacgc
    30181 ccatcagggc ttcatgcttc acctgcccgg tcttaaattc cgccagtgcg ttggaataaa
    30241 aattccagtt gatattccac tcgtcgctcc aggcggcttc atatttggcc tttccggaag
    30301 caatcatctg gttgtaataa tccgccgtgc tggttcctct cccagcggaa gaggaaatgc
    30361 cccagacatt atagtccaca tcgctgtacc caataccgcc gccaatgcgg atcgtccata
    30421 tcttttccag cttgcgttcg aaatccagca tcgccagcag ggatttggaa ttaagccttc
    30481 cgctcggaga accgtaccaa gcgtcatacg gccccacaaa atggccgccc agcacaggga
    30541 tgcccatggt cgtcggagaa ttctgatact ggaaactggt cgtcagcacc gtcttggtgc
    30601 gggaatcatg ctgccagcgg aaaatggggg acaccgtgta cttctggcct ccgttggctc
    30661 cgttgctcag ccagaacggg cgttcatact cggcggctac caccgtacgc agggcaaatc
    30721 cattcgtttc atcccccctg taccgggtat cgtcaatcgt tgcgcggtat ttctgcccgt
    30781 gatgggaaag gcgggcgtaa gccgtcaact ccgtccgttc caggaaatca ggctccttca
    30841 ggatcaggtt gatggaaccg ccggcgccgt atgccccgag cgtactggtc tgaccgccgg
    30901 aaatggaacc gatggggcct ttgacaatct caatattttc tatcagggac gtatccatgc
    30961 cataccccat gcctcgcggg aggggcatat tgccgatctg aacatcactg ccggcaaagc
    31021 cgcggatggt gtactggtcc gccgtacggg acaaaagcat gtccccaccc gtattgacgg
    31081 aggaatccat acgcagcgct tccaccaggg aatcggcccc cttggaatcc atcaggtccc
    31141 ttgtaatgac gtttaccgtt tgcggaactt cctccgtctt catgttcgtg agagtagcgg
    31201 aactgaccgt ttccgcccgc agggacctgg aacggatggt catcacgcgt tccggcattt
    31261 cctgcacgcc ttcctctgct gcggcggcct ttgtctcggc ataggcagat tgtccgagaa
    31321 caagcaggct cccaatagcg gcagcacggt gcaggccgct cccctggcgg cgatggcggg
    31381 gggtattcat gttggataaa cgcatagggg caagtatagg acggaacaaa gaaccgtcca
    31441 ttcccgtttg ttaacaattt gataacagac cgttcctttt ccttccggca aaagcgcatc
    31501 tgatggcaga acggccccgc cctttcacaa gcccaaagcc tgcgcccggc tctccgccgc
    31561 ggccttcagc cattcccgca atgcctcctt atcccctgca gccagccaat tcctcacttg
    31621 tcccagctgg gacagggctt catccagacg ctccagaaca gcaggagcat tttccgccag
    31681 aatttcagcc cacatggacg gttcccccat ggaaacgcgg gtagtatcac ggaaacctcc
    31741 ggcggatacc agccccagca gcttcacatc cccaccgtcc agggcgctgt ggacgcacag
    31801 ggcggacaaa gcatgaggta tgtgggaaat gcgtgccacg gaagaatcat gatccgccgc
    31861 tttcatccgg atgcagtggc agcccacacg ctcccagaac cgctggagca acagcagcac
    31921 atcttcatgc acatgctcgt cattcgtcag gatgcaggtc gcatcccgga agaggtcccc
    31981 ggaagcatgc tccataccct gcttttcgga accggccatg ggatgggagc cgataaacgc
    32041 cacgccggct ttcttcagca cggagcccac ggcctggtga acacacccct tcacggaacc
    32101 cacatccgtt accagtactc ccggtttcaa taaaggaagc atgtcggaaa caaggcgggg
    32161 catcacgcca accggggtag ccagcacaac cagatccgcc ccctccacca cctcttccat
    32221 ccgggtggaa gcggcatggg cggctccgga agaacgggca tattccaacg gttcttccct
    32281 cctgccccac aagcgcacgt gcacatgcgg caactgcttc ctgacagcca gcgccaagga
    32341 cccgcccaga aggccggggc cgagaatggc gacggaggag aaaaaaagct gctttctgga
    32401 atattctctc atggatttac agggaaatac atcagcgccc cggaacatcg gcatgttcag
    32461 gggcgcggcg tgatctcgtt gcggcggccg catcagggca cgcggaactt cttgatctcg
    32521 ttggggaagt tggtgtcccg caacaccgtc ccggagggat acggcttatt ggtgctcctg
    32581 ttcaaaatct tgatcttctt ggtacggtca tacgggctga ccaccaccgt gggatccgcc
    32641 gtaggccacg ccaccgggat ggaagaagaa gcgggaggtt ccacaggcct gggggcggca
    32701 atgccggaac cggcggagtc acccacggga ttaggaacga cgggcttggg aacaacggga
    32761 gccggaggcg tgatattgtt ggccccgccc accatattgc tgatggttcc ggaattggtc
    32821 cgggcgggat ccgggttaaa cccggaattc tgcccggcca tcgggtccag agaattttca
    32881 cgctgaatat cattcaacag gctattgccc tgggcgccca tagcgccgga cggatagaca
    32941 ttttccgtag gataaatgcc gatggggcgt tcattcacgc gctccgtttc acaggaaggc
    33001 agaagaattg cacctgcggc cagcagcacg cctatcgtcg gggaagtatt catataatct
    33061 ctcttcgggt gatagctttc tgccagcatc ctacggtccc gcaacctcat gtcaaccata
    33121 aagcctgttt gccgaacctc ttcatgacaa ggaatcttcg gcttccgtcc ccgcacgcgg
    33181 gcaaagccgc ccgaacaaac gcgctcgccg catttctttt acccgccggc agcgccggaa
    33241 cagccctgga cgggctcaaa aagagaagag ccgcaaaacg gccgttttac cgcagacagg
    33301 cgtccgaata gccggaaagc ggcacaaaga cggaacaacc atcactcctt atcaacaagc
    33361 gctccaacaa gtactcctgc cgcagcctta ttcggaagaa accgaagctc catcccgttc
    33421 gccagcggga agagacgctt ccctcccgaa catgccgcgc cggcctccgg ccctcccctg
    33481 cccgccctct tcaaaaaccg gaaaaacgct tccccctctt cccgggaacg cgcctgtacg
    33541 ggaacagatt atccttccct accccccatg atctgctaca atccgcccca catggatgac
    33601 gtacagacat ctaagaaaaa aatgccctca aaaaaggaat ggaggggatt ttattccctg
    33661 attctcattc aagcccagaa cgctttcaat gaaaaagccg cccagttcct gctgattccg
    33721 ctgggcgtct ggctggcttc caccaacgcc gcatacggac cggattcctg ggtcaactcc
    33781 ctccagtaca ttctggcctg catctttgtt ctgccctata ttctgttctc ccctttcgtc
    33841 ggatggctgg cggactgctt ctgcaaggcc cgcatcatcc aattcatgtc tttcctacag
    33901 attctggtac tgggggccat gcttctgtgc tataaatacg aaaacattga aatggccgtt
    33961 ttctggttct gcgtcttctc cgtccaggcg accatcctga gtccggccaa aaagggagtg
    34021 gtcaaggaca tggtaggctc ccggcagctg ggctacgctt cagggctgat ggaaatgagc
    34081 ctcatcctgt ccatgctggc ggcccagata ggcatcttcg cctggttcga catcctgcaa
    34141 gtctcctcca atgacggctg ggaagcagcc gctttcccca cattcatcct cacctgcatc
    34201 gccgttcccg tagccattgc gtccctttac ctcccgcggt atccggccaa ccagaccaga
    34261 aaattcgaat ggaagctctt ttacgaacat ttcgtgcagc tcaagtatct ctggagccag
    34321 cgggatttga ggctcagcga aatcggtgtt tcctacttct ggttcctggc cggagccctg
    34381 atgctcatct ccctccagat cgcccaggaa caccccattg acggcaccgg attcagcatg
    34441 tccgcggcca ttctcatggc atggctcagc ggcggcacgg tggtaggcgg cgtcatcgcc
    34501 tccatcatct gccggaaaaa aattgaactg gggctcatcc ccctgggcgc aatcggcttc
    34561 actatcgggt gcatgttcat gtccttcttc gctcccggct ccctccccag caacatcggg
    34621 ttcggcatca ccggggcttt cgccgccgcg tacctggtgc cgttaaacgc ccacctccag
    34681 gacaattgcg acccctccaa ccgcagcacc gtaatcgccg ccggcaacct gatggactgc
    34741 ctgatggggc tggtggccgt cggcttccag ctcatgctca ggaacatctt ctccatccag
    34801 aaccaattct gggtgctggc cgccctgggc gtcgtcatta ccattgtggc attccggctt
    34861 attccccgtg aattcatccg catgatgggc ctctggatca tgaggattgt ctaccgctcc
    34921 cgcatcatcc accaggaccg cattccggaa gacggcgggg ccgttatcgt ctccaaccat
    34981 gtcacctacg gggatgcgct gttcctttct ctcatttgcc cgcgccccat ccggttcatc
    35041 gtggcggagg aattcgtagc catccgctgg ctcggctgga ttctggaact cttcaactgc
    35101 ctgcccattt cctcccgtaa tccgcgggaa tccctgtcca aggccatcca ggccctgaag
    35161 gccggagaag tcatctgcat cttcccggaa ggccagctca cccgcacggg aaccctgtgc
    35221 gccgcgcgcc gggggctgga aatgctggcg aaaaaatcgc gctgccccat cgtgcccatt
    35281 tacatggacg aactatgggg cagcatcttc tcctactcag gcaaccgttt cttctccaag
    35341 gcgcccctcc gcgttcccta ccgtttcacc gccgccatcg gagagcccat tgagcctgat
    35401 gccgtcaatc cccgcatggt catcaacacg ctgcgggagc tatcctccac atgcctggaa
    35461 attgcagcca gcatcggccg ggatgccatc ctgaaccatc tggagcgcat cgcccacaaa
    35521 ccgctggtct tctctaaaaa cgcgcggctc tccgggtatg aaatagcgga atgtctgatg
    35581 aacgacaccg tggaagcaga gcatccggaa ctcaggaaat ggctgtccac cctgctggac
    35641 agcacccgct cccaaagccg cctgtgcgac ttctggatga acgcccagca gctggaacgg
    35701 gtgaactccc tccagccccg ggaactgctg ctcaccagcg taggacgcga agaagtccac
    35761 gaaacggtgg ccgccgtcct gtggcccatc ctcaccagca ctcccgttta cctcatcagc
    35821 gacggggacc acagcatgcc ggaaggcatc aggcagatag ccggctccga tttcctgcgc
    35881 cgcaggctct acagcctggt tccgaatacg cgcacgccgt tttatgactt cagcggctcg
    35941 ggcgacctgg ttctccccaa catcggctgg aggccctgct tcgccacgga ccgcggcatt
    36001 atcctggcca tgtccatgaa gagaagcgtc ttcaagctgg atgacggcac cgtgcagctc
    36061 ggcatgcggg ccaggacaag agggcgcctc ctccccggct tttacctgaa tccccctctt
    36121 tccaacgtca tcgccggggc taccctctcc accccttatt ccctgccgcc caacctgtac
    36181 ctggacgagt cgggcttcct ggcggaactc cagtcttcca accatgaaca ataactcctc
    36241 gtcaacccgc cttcccatcc cgcagtatgt aatgacgctg gcgcacgcgg cagccctgcg
    36301 gtcggaggac ccgtaccgca aggtgggagc cgccgccctg gatgcggaca accgcgtcat
    36361 cggcaccgcc tacaacggac tgtatcccgg attcaaggct caggacacct tctgggccag
    36421 ccgggaagaa cgccagaaat acatgctgca tgcggaaatc aacctgtgca gcctgttccg
    36481 gaggggggaa gccaaggtgg tggcatgcac tacgatgccc tgtacctcat gcatgcaggc
    36541 gctgtgtgcc catggcgtaa aaaccattta ttactgtgag ccttatgaca aatccgaagc
    36601 tcctgccatc gccagccttt acggtgtgga actgatccag gttacggact atcctctcag
    36661 ccggcacttc ccccttgtcc tggacagctg atgcccggaa aatcaatccc ggtgtacgcg
    36721 gcatgtccaa ccggccatgg agcggggaac gccgtcaatg gcagggtcca aatcaagacc
    36781 ggtttccgta atggtgcccg aagtaatgac acccgcattc cgcccggcgt cgtcaaaggc
    36841 aatgttcaca ttcgccatgg tacaggcgga gctgctgttg gctgccggag caatattcaa
    36901 tacgccggca ttctccccgc tattgaaccg gtacgtataa accccgtcgt acacggctct
    36961 tgtatccatt ctgataacat ctacgtcctg cccgctgaac tggagctgga ataagatgct
    37021 tcctccctca aacaggctta cctgctgtcc gctcagggaa gtgttcccgt cgtccccgga
    37081 ttttcccgaa cctccgccgc acccggacaa caatccgcac accgtcacag gaatcagcaa
    37141 caagacaggg aacaactttt tcattcccgg aaaaatgcac aacagaaaac cacaagtcaa
    37201 gtatactcaa ctattgttgc atttttccaa cattttttaa catcacaaaa aaaaccgcct
    37261 gttttcacag gcggtctcaa gaaaacacgg aagcggtcag ccaacccact tccaggtaat
    37321 gggtatttca atatcagggt gcaggctgtg cttgaccggg cagctcagag cagcctgttc
    37381 cagcagcttc ctgtccggat aatcggacgg aagaggaacg gtaatgacga cttccagctt
    37441 gccgatacgg cggggatccg cattcatgtg tttggccact tccaccgtca tgccttccag
    37501 attaatcccc ttacgttcgg caacaatacc catgatcgtg ctcatgcagc cggcaagcgc
    37561 cgctcctacc aggtccgtgg gggaaaagga ctcgcccttg ccgttgttgt ccacgggggc
    37621 atccgtggaa agcgtggccc cggaaggttc atgaaccatg gaacaatgga ggccgccctc
    37681 gtacgtcgtt ttagttttta ccatggcctt attttataca aggctccccg ccatgcaaca
    37741 cacaaacatt gaatcccccc gttctttatg gtaaccgcct ttgctatgag aaactttttg
    37801 aaagcagccg tcggctgcct gggcatgatc gccgggtgtt tcagcacagg ggaggcccag
    37861 gacttcggag gcatgagctt cggggaagcg ggaaactttg gtccgcccca gacatccggt
    37921 tcctccaagg ccactgtcac ctcctatgga gaagcctcct tcgtcatcgt gacggagctg
    37981 gcgctgccgg accactggca cgtttactat aaaaatccgg gaaccgtggg ccttcccatg
    38041 gaagccgctc tccagcctgt gcccggcttc cgggtggaag gccccttctg gcaaatcccg
    38101 gaactgggga aagggcttgt ggatttttac ggatacagcg gaaaagccaa aatggcgttc
    38161 cgggtgactc cagaaaagga tgcccccccg gaagcgacgt tcaccaccac catgacatgg
    38221 caaatgtgcg cggaacagtg cgccgccccg gaaaccaaaa acttcagcgt cacactgaag
    38281 cgcggagacg ggcagacagc ccctgacgcg gcggaattga ccgggaacat ggcagggctt
    38341 gccgcccccg actgggcgga agggctgaaa gcccggattt cccaggaagg gaaaaccgtc
    38401 accctgcacc tcaggacaaa cggacgcccc gttccgcagg attccgtcta ttttttctgc
    38461 aatcaggggg aaatcaaccc taccactccc caaatcttca aaaaactgga tgactccaat
    38521 tatgagctgt ccatgcaatt caatgacacc acggacggcc tttaccccaa caacctgccg
    38581 gatgcagaca gggggaaacc gctgaccagc ctttccggca tcctgcgcgc cggcagggaa
    38641 ggcatcatca tcaccgcgga cgaccgcccc ttctccgggg aatctcaggc aacagccgcc
    38701 ggaacggaat ccgccccgga atcttccgtt tccatccccg cttctccgct gatgggcctg
    38761 ggggaaatca tgttcttcat gttcatcggc ggcatcatcc tcaatgtcat gccgtgcgtc
    38821 ttcccggtaa tcggcctcaa gattatggga ttcgtccagc tgggaggggg ggaacggaaa
    38881 aaagtgctgg cccactccct taccttcgta ctgggcatcc tgatttcttt ctggctcatt
    38941 actgccattc tgattgcgct gaaagcgaac atgtttgact ggagcgcccc agccggcccc
    39001 ggcgtgttca gcggagactt ctggctgggc cgcggcgcgg agggcgttgt caactgggcg
    39061 ttctggtttg aaaacccctg ggtgaatttc tgcctgctgg gcctgatgct cgccatgggg
    39121 ctgagcatgt tcggcgtctt tgaaataggc gtcaaagcca cgaccatggg caacgacctc
    39181 cagcaccgga agggttatgc cggctccttc tggtccggcg ccctggctac ggtcatctcc
    39241 accccgtgca gcgctccgtt cctgggccag gctatcggcg cggccatgct ccagccgccg
    39301 ctgggcatcg tgctctgcct gaccatgatg gggcttggaa tgtcccttcc ctacatcatt
    39361 ctgggagcct ttcccgtcct gaccagatac ctgcccaaac ccggcgcatg gatggaatcc
    39421 ttcaagcagt ccatgtcctt cctgatgttc ggcaccgcag cctacttcct ctggatttac
    39481 atggccttct ttgatgcaga aaaccatcct caggacatcc tgttcctctt cttcgggctt
    39541 gtgctgtttt ccatggcctt ttgggtatac ggcagatggt gccccatata ccgcagcaga
    39601 aagtcccgca tcacgggagg cgtcttctcc gtaatcttcc tgctggccgg cctgtactac
    39661 atgctcccgc cggaaggagc agcctggttc ggccgcggtt ccgtccccgg ggcggcggag
    39721 tcctccgctg ccgcagcacc ctcccttcag gaggaaggaa acatctggat accctggagc
    39781 ccggacgcca tgcaggccgc cctggacgga gggaaacccg tttatgtgga cttcacggcc
    39841 cgctggtgct ccacctgcca agtcaacaag gcctcctata cggatgaagt gctggccgct
    39901 ttcaaaaaat atggcatcgt catgatgaag gcggacaaga cccgcacaaa tcccgccatt
    39961 gaccaggaac ttaaaaacct ggggcgcacg gccgttcccg tcaatgccct gtatcttccc
    40021 ggcagaaaac cagccgtcac cagggaactc ctgtccccgg cctacctgct ggaattcctg
    40081 gaaaaggaaa tggaacgcta ggacgtcttg tcatgcgggc ggcaatccgc cgccctcccc
    40141 ccaggccatg ccgggcagca ggaaacgggg aaagcttacc cttccccgct caacagcggc
    40201 agcaacgcgt ccgcccatgc ctgataaccc ttctccgtta aatggacatg atccttgaac
    40261 atgccctccc tgaacgtccc gtccggcttc agcaggctgc cgttaatgga taaaatagtg
    40321 gtcctcggaa gctcaaacct gcgcagcaaa cggttaatct cctcctgcct cctgcgcaat
    40381 gggctatcgg gatcggggcc gtagggagtc gcctccatca ggatcaaacg ggtctccgga
    40441 aatctcttca gcaggcgtac ggctatctcc cggatgccct tcaccgtctg cgccgcgggc
    40501 tgccgccctt tccacaggcc gatattattc gtcccggcca gcaaaatgca atagcgcgga
    40561 gaagtcctga ctgccagtcc cgtatcttcc atgcgccaca gcatgctgtc cgtcctgtcc
    40621 ccgctcactc cgaagttgac cgccttcatc ggcacgaaca gcctcttcca cagcgcggaa
    40681 ccttcccgtt cccagcattc tgtaatggaa tcccccacga acagaatgcc gcattccttc
    40741 cccagcaaat tttcccggac cgccttgtaa cgggccggaa cccacggctg ggtattcagt
    40801 ttgggaacat cgggcggatc cggaacctca tctgaaggta ccgcatacac ggcagaagaa
    40861 gcaagggaca gcaataaaaa agcggcggaa aaaacagaag cattcatctt cttccgaata
    40921 cgcggcccgg aaaagcattc tttcccctgc cgggaaaatc atgttctcac cgccttgccc
    40981 aatacctcca ggggctggcc ggacaaattc aattccttta ccagaaaacg gacaatcagc
    41041 aggcttgcca gcgcatcata caaagcgtca tgccaccggc gcgaaggaag cagagcctcc
    41101 acggaacaag tcagccccag ggcgccgcac aaagaagaca gggaataatc cgccagaccg
    41161 ggaacgcaca tccggcccag ggccagcgta tccagccacg ggccgaagcc gtggcccgga
    41221 aaccggcgca ggaacttccg ttccgtggcc ggattatgcc ccaccaccac ggactgcccc
    41281 agccgggaac gtatctccgg ccacagggag ggaaaagacg gggcatccgc cagcatctcc
    41341 gtagtaatgc cgtgcacctg ggaggcggac cataaaaccg gcttctccac cgcaatatag
    41401 gaagtccaca actcaggctc gtcctccagg cggggacatg cggctatgcc tatctggacg
    41461 ggctggtccg tctccccccg ggccgctccg gcggattcaa aatcaatggc ggcgaaatgc
    41521 aggccgccga ttgtatccga aagcagaggc atatcagaac aactccagct gagcatattc
    41581 cccggcaggc atatcgtctt ccatatccgt attcttggct ctcggttccg ccagccggga
    41641 gcgctccttc ctccgggggc gcgtagaatt catctccaga tgggacagga tggccttggc
    41701 gcggtccaca atgacggcag gcatgccggc cagtcgggcc acctggatgc cgtaggactt
    41761 atccgccgcc cccggcacga tcttgcgcaa aaacacgatt tcctctttcc actcgcgcac
    41821 ggccacattg taattctgca cgccctgcct ggaattggcc aaatccgtca gctcatgata
    41881 atgcgtggcg aacagggtgc gggacttcaa ctcgtcatgc aggtactccg ccacggccca
    41941 ggcaatggaa agcccgtcaa aagtggccgt gcctcgcccg atttcatcca gaataatcag
    42001 ggagcgctcc gtggcattat tcagaatcag ggaggtttcg ctcatctcca ccataaaggt
    42061 ggactggccg cgcgccaggt cgtcgctggc tcccacgcgg cagaaaatgc ggtccaccaa
    42121 accgatgtgg gcggactccg ccggcacata cgctccaatc tgggccatca gcgtaatcag
    42181 ggccacctgg cggatatagg tgctcttgcc cgccatattg ggcccggtca gcagaataag
    42241 gcggttttcc tccggttcca gaaaagcgtc attgggaacg aacacatcgc cggaaacatt
    42301 ctgctcaata acgggatgac ggccattgac aatacgcagg gtcatggaat tgtccagaac
    42361 ggggcggcaa taccggtact gctgggcccc ctccgccagc cccagcagca cgtccaggtc
    42421 cgccatggca tccgccgtaa tctggatatc gtcaatgtgg cgccccactt cctcgcgcaa
    42481 caggaggaac tgctcatact ccacctggcg ggaacgctcg tccgccccca agatggtatt
    42541 ctccatctgc ttgagttccg gggtaataaa gcgctccgca ttcaccagcg tctgcttgcg
    42601 ctggtaatcc gggggcactt tatcgtaatg gctcttcgtt acttcaatgt aatagccgaa
    42661 gacgttattg aagcggattt tcagggaatc aattcccgtg cgcttgcgct ccttctcctg
    42721 cagtcgtgcc agccactcct tcccgtcgcg ggaagccaga cgcaattcat ccagaccggc
    42781 atggtaccct tccctgatga tgcctccctc cttgatggtc acgggaggtt catccaccag
    42841 agcgcgctgg agcaaatcca ccagctcatc aaagcagccc atgcgggaac ggatgctctc
    42901 cagcatgtcc ccgccgccgg gcagggattc cagatcatcc ctgagcgcgg gaatgcgcgc
    42961 cagggaggaa gccagcgcct ggaggtcgcg ggcattcccg gcgccctggg aaatgcgccc
    43021 cgtcagccgc tccatgtccc gcacattctt caggctctcc ctcagcttgc tcatcaggta
    43081 gggttcctga agcagaacgg cgatcacctc ctgcctcgcc aggagctttt ccagatcgca
    43141 cagggggtgg agcagccagt cccggagttt gcgcgcgccc atgggggtgc tcgtcctgtc
    43201 cagggtcccc agcagggaaa gcttcacgcc gccgcgggaa tccaccaggt ccagattcct
    43261 ctggctggcc tggtcaatca gcacggcgtt ctccgtggcg cgcacggaaa tgcggcgcaa
    43321 gtggtccgtg gggcggcgga gctggtagcc cagataatgg agcaccgcgc cggacgcccc
    43381 cagggcggcg gtcatctccc cgcagccgaa accgtccagg gaatgcaccc ggaaatggtt
    43441 cagcaaattg ggaatggctg tggaaggcag aaaagtatat ccgtcgtaat aaagcgtggg
    43501 gtgggttccg gggaagcagt ccgtctgctc gtcgctgacc aggagttcgg agggattgat
    43561 gcgggacagc tcgtccagaa gcaaatccat gtgttcgaac tgggccacgg aaaattcccc
    43621 cgtggtatgg tccacgcatg ccaggccgaa gcgcttcttg tccttgtaca acgccacaat
    43681 gtaattatgg cgggaggaat ccagcaaatt catatccgcc agggttccgg cggaaatcac
    43741 gcgggtcagt tcccgctcca ccagcttgcc cggctgcgga atggtggtct gctccgcaat
    43801 agccacccgc tttcctccct tcaccagccg tccgatatac ccttccgcgc tgtggtgggg
    43861 caccccgcac atgggaatgc cgtggcgctt ggtcagcgtc aggcccaaaa tggcggaggc
    43921 ctccttggcg tcctcaaaaa acatttcgta aaaatccccc agccggaaaa acagcagcac
    43981 gtcttccggc aagcccttct tcatgcgaag gtactgatcc atcatcgggg ttgaggcagg
    44041 tccggaacta ctcataaata acggtgaaag agtaatccga ttgcggcttc aaggcaaggt
    44101 tggaatcttg ttagaaccgg ggctccactc cccttttcca aggaaaagcc agggaagaat
    44161 catccaaagc cccgcaaaga aaatgcgttg cctctgccat tatcgcgtct tttctcttcc
    44221 tctccggaaa caggccctca tgcggaagaa cccggccatg cagaccgcca tctttttgcg
    44281 aagcgcggca aacaacaggc catttattcc agaaaagaaa aaaccgtgcc gcaaccggat
    44341 gcggcacggt gtgaagtcag attggagtaa ttaatattcc accacggggg catccttcca
    44401 cagggtgtcc attccgtaga attcgcgcgt ttcctgcccc atgatgtgaa ccatcacgtc
    44461 aatgtagtcc actacggacc acaatgccac aggcgtatct tccacataag tggggagggc
    44521 tcccgccttt tcccggacgg cttcttccag ctcccggatg acggcgcgca aatgggggac
    44581 ggacaacccc gtgcacacta ccatgaaatc cgtcagggaa gacatgcccc gcacgtcgta
    44641 cacccgcaca ttttcggcct tggcgtcatc cgcggcgcgg gcgcacatcc tggccagttc
    44701 catcgcatcg ttggctacca tattcgattc gtttctattg aattatttct tttctccgcc
    44761 gtccttgccg annnnnnnnn ngaaggggtc ccgttccgcc tgttccagtt cctcttccat
    44821 cttccgttct tcagcgccgt ccgcgcgggt ctcttcagct tctttcttcc cggtatggcc
    44881 ggaatcatcc ttcccgggct gttcctccac ttcggaaggc atggggggcg gagtcaccct
    44941 ggagggaggg tttttcattt ccccgtattc cagaatatcc atgacctggg aaccttccag
    45001 cgtttcaaac tccatcaggg cctccgtcag aatgtccagc ttgtcccggt tttccgtcag
    45061 gatggccatg gcgcgttcat aagcgctgtc caccaggaag cggacttccg agtcgatcag
    45121 ctcggccgtg gattcggaat aattgcggga acgcgtcccc aggtcgcggg caatataaac
    45181 ttctccctgg tgttctccgt actcaatcag ccccagtttt tcgctcatgc cgaattcgca
    45241 caccatgcgc cgcgccaggt tggtggcgct cttgatgtct ccggtagcac cgctggtcac
    45301 atcaccaaaa acgatttgtt cggcgcagcg cccgcccatc gccacgacga gctggtccag
    45361 catttcggac cggagctggt gcatcttgtc gtcggaagga agccacatgg tcatgccgag
    45421 ggccccgccg cggggaacaa tggtcacccg gtgcagcggc tcgctgtgcg gcgttttcaa
    45481 caggcagatg gcatgccccg cctcatgcac ggcggtaatg cggcgccccc gttcgttaat
    45541 cgccaggctg cggcgttcac gcccccagct gaccttgtcg cgggcttctt ccaattcggc
    45601 ctccgtaatc tccttcagcc ccttgcgggc ggccagcagg gcggcctcat tgaccaggtt
    45661 ggccagctgg gcaccggaaa agccggacgt gccgcgggca atccgctcaa agctgactcc
    45721 gggcgccatc ttcacttttc tggcatgcac ctgcaggatc tgttcgcgcc cccggacgtc
    45781 cggcaggttc accaccacct gccggtcgaa gcggccggga cgcagcaggg ccgggtccag
    45841 gatgtccgca cggttggtgg cggcaatcac gattacattg gagttgtttt caaaaccgtc
    45901 catctccacc agcagggcgt tcagcgtttg ttcccgctcg tcattgccgc cgcccatgcc
    45961 gtaaccgcgc tggcggccca cggcgtcaat ttcatcaatg aaaatcaggc tgggtgccgt
    46021 ccttttggcc tgttcaaaca tatcgcggac acggctcgca cccaccccca cgaacatttc
    46081 cacaaagtcc gagccgctga tggaatagaa ggaagcgttg gactccccgg caatggcacg
    46141 cgccagcaga gtcttgcccg taccgggcgc cccgaccatc agcacgccgc ggggaatggt
    46201 ggcgcccaga tcgcggaatt tttccggatt gcgcaggaac tccaccagtt cccacacttc
    46261 ctccttggct tcgctgatgc cggccacatc cttgaacgtc accttgttct tgtccgggga
    46321 gatgaggcgc gcccggcttt tgccgaagct catggccccc cgggccccgc cgctctgcgc
    46381 gcggaacatg aagaacagaa tcaccagaat gagcacgatg ggcagcagat tcagcagaat
    46441 gccgccccag gaaccggatt cacgcttgaa cttcgtatcc ggccccagca gctgcttgac
    46501 gcggtctccc tggaactcca gggtaaaagg cacttccaca cgttcaaaac gctgtttgtc
    46561 cacggaggcg tctcccgtag ccgcgggcca gatgcgggtg acaatctgtc cgaccagcac
    46621 gttctggttg ccgtcttccg ccaggatgat gccgtccttt cctccggcga tgcggccttc
    46681 cagggccaga cggttgaatt caggcacgga aagcttctga gctccctcag gaatgaggga
    46741 aacattcttt ccctcttccg tccggtacgg gctctccacc accctgtagc cggaaagttc
    46801 attcagcaaa ggcttgtcgc ggtccggaag ggacatggaa taagtcagcg caaagggggt
    46861 catttccacc ttcggctgaa tttccttcct gtacaccagc gcgtgaataa cgccttcgga
    46921 accgttctcg ctcagcacca cttccacggg ggccttgggg tcgttcagca ccacgcgccc
    46981 cgnnnnnnnn nncgccccgc tttatactgg gcttcaaagg attccagatt ttccttgcgg
    47041 gggcccaacc cgaaagattc aggggtgaaa aagccaaacg caaggacgcc tacaatgaga
    47101 agtaccatga cccacacgcc ccagttgggg gattcgggcc tgccggagcc gggaaatttg
    47161 ggaggacggg gaggactggg tggcattctg tcagacataa tgtggttagc acacagaagt
    47221 taatgaatag ggcggcatga tacgcgagtc cttgtaaaat aaaacttttt tccgagacat
    47281 gccatacagc cgccgcagga cgggcccgca ccgttccggg ccggggctct tcccggcaat
    47341 aaggccttaa ccggagcagg cctcattccc gctttctttt tctgcgccgc ccctcatggt
    47401 tcgcgctttc tccgttcatg cgctgccgga gacgccgctt ccactggggc atcctttctt
    47461 ccgccggggc cgcatgggaa ccggcatctt cccggctctt ttcctgccag gccccctcct
    47521 cctgcgcggt cagccgctcc cgccgcctct gctcctggat ttccgccaat tttctggcgc
    47581 ggcggatttc cacccgcttg ctcacctccc cgtgggcgga ggtcacgcgc caggggccga
    47641 tgcggcgggg ataggtaatg ggtgcgcaaa ccagttccgc atccagaaaa cgcatggacc
    47701 ccaccagcag gttcttgaca ttcgccagca ggccgccgtc cgtccagacg ccgcccatgc
    47761 ccatttccag cagaaaggcc agatgcagcg tctccgctcc cgaggcctga atggaaaaat
    47821 ccctcaggga aatgccctcc gccggctcct cgtccggagc ggccacggaa gcggcccgga
    47881 ggaggaacat cgtatcccgg accttgcggc tcagggcgca gaccagttcc cccaccttgg
    47941 gccgggcgtc aaaacggtaa tccttcactt ccaccagcca cagatcccgg cgttccggat
    48001 gatacagcag aaaatccatt tccttgcatc ccccgcagaa attctgcgcc tgccgggcat
    48061 aataatccga annnnnnnnn nttcaatgcg gcacaccacg tagccttcat cgatccagaa
    48121 acggtggacg ccctccacgt aataagcttt tgacggctta tgactcataa ctcagcttca
    48181 aatacctgtc cgcctgttcg atttccgctt caagagactc gacaggtccc acgtcctcca
    48241 gggattcccc ccaggacacc cttaccccgg aacgttcccc ccgcggcacg ctcatcccga
    48301 aaaactttct ctgcaccatg gcattgcggg gttcggaaag ctgaatcatc agctcccgca
    48361 gcaggaacag gctgtgggag gacaaaataa cctgtacccc cgtcttggca agccccgtca
    48421 gggttttcac cagcacgggc aggtgggagg cgttcagatt catttccggt tcatcccaaa
    48481 acaggacgga accttttttc acggaaccgt tccggatcag gtagctcaac gttcccagcc
    48541 ttttgaatcc ttccgccacc aggctcattt ctatcggctg ctgtccggga cgctgcaaaa
    48601 agaaccgccc gtcacgcttc accaccttcc ccccgatgat tttttccagc ctggcaacca
    48661 cgcgcccggc atccgtactg atgggctccg cttccgcctc catatccaga taacggcaga
    48721 ggtcccaact gccgccgtcc agaaattcgg ggaatctgct tcccacctgc acaaaactcg
    48781 gataaatggt cagcacctcc cgcgccgcca ggaacaccac cggcacgttc agaaaacgcc
    48841 ggggcatttc ccggatgctc agcccctcct cttcatgccc cgcctggaaa tcaaacacca
    48901 ggttccccat gccctccggc acgccgtccc cctccatgga ggcctccact cgcgcatggg
    48961 cgtttccccg gttccgggcc gtcagcccgg ccagcccgcg ggacgcgaac acccgcatca
    49021 ggtcttttct gagccgctgc tcctccgccc atttttccgg cagggatttt ctccccccat
    49081 cagcgctcca cttggccacg gtatagcata atttcagcaa atggcttttg ccggaatcat
    49141 tgcctcccac caccacattg attcccggaa caaactcaaa atgctctctc ccgggaaaca
    49201 ccgtcacgcc gctcaccagt aggtcatgta tcataccgtt cccaatgtag cacagaagcg
    49261 caggccgtaa atgacaaaag ccgccagctg tcggccaccg gggagcctgc cggaaaaaca
    49321 ccggaaacaa cggggaaaac gcatacggcc gcagcaccgc cccatccccc gcaggttccg
    49381 gacatacccg gaaggcggcg cattttaggc ttcccattgc cgggcgcctt attaaagtaa
    49441 tccgtatgag cgcacaatgg accaccgcca gggaatacac ggacatcaag tatgaaacaa
    49501 cggacaacgg cagcatcgca aaaatcacga tcaaccgccc ccacgtccgc aatgcgttcc
    49561 gtcccctgac cgtgcatgaa atgctcaacg ccctgaacgc cgctcatgaa gaccccaagg
    49621 tgggcgtggt catcctgacc ggagaaggcc cggacgcttt ctgctccgga ggcgaccaga
    49681 aagtgcgcgg aaatgccggc tatatcggtg aagacggcgt gccgcgcctg aacattctgg
    49741 acgtgcagcg caccatccgc accatgccca agcccgtcgt cgccatggtg gccggatacg
    49801 ccatcggcgg aggtcacgtc ctccacgtcg tctgtgacct caccattgcc gcagacaacg
    49861 ccaaattcgg acagacaggg cccaaagtgg gctcttttga cggaggcctc ggctcctcct
    49921 acctggcgcg catcgtgggc cagaaaaaag cgcgggaaat ctggtacctg tgcaggcagt
    49981 acgatgcccg gcaagcgctg gacatgggcc tggtcaatac cgtagttccc ctggaggacc
    50041 tggaggagga aacgctctcc tggtgccgcc agatgctccg ccacagccct ctggccctgc
    50101 gctgcctgaa ggcatccctg aacgccgact gcgacgggca gatgggcctg ctggacctgg
    50161 ccggcaacgc caccctgctc tattatatga gcgaggaagc gaaggaaggc aaaaacgcct
    50221 tcgttgaaaa acgcgctccg gatttttcca aattccccag acttccgtaa cccccctcct
    50281 ccgcaccatg gcacccagct ccgacaatga aggcgccccc cggaacggca ccgccggcag
    50341 ggaattcctg cgcgacctgc cggaacatga aaacatcgtg gag
//
LOCUS       DAWEMX010000005        48798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_322_length_48798_cov_13.548633, whole genome shotgun sequence.
ACCESSION   DAWEMX010000005 DAWEMX010000000
VERSION     DAWEMX010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 48798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 48798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..48798
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_322_length_48798_cov_13.548633"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4081..4090
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    40713..40722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10891 a        14692 c        12444 g        10751 t
ORIGIN      
        1 tcattatgtg cattatctcc accattgaag cattcattat gtatttttgt tcttgcatca
       61 atacagtctt gaaatctttt tatatttctt tctatttcat ttttacacat atttttgttt
      121 ttacaatgca ttgatgaacc tttgcactta cgaccaacct ctttatttaa ttcactatgt
      181 ctttgttttg aacaatttcc atatctaggt ttttgaggaa ttgctattgg aactgtttct
      241 ggaactgttt tctttgtagt atccgagctt atttcatcta ttactttttc ggcaactacc
      301 accactaccg caacagctac acctactgcc gccgccgcta ccaccgccgc tcctatagga
      361 ttaaatagag caatcgcaaa cgcgaattgc ccctgcacat caatcaaaat agaaggtgtg
      421 ttatacgcat atgaataaac attatctttc tgtttctcta taataggatc cctactaatc
      481 cacctgccat tttggggatt ataatagcgg taattgtagt aaaccagccc cagttcgtca
      541 tcagcgtatt cgctggaaaa ccggaacgga ttgtcctctg cggcattccc ttccatcctg
      601 agaatattcc catacgggcc gtattcgtac aaggcgcggc gtcccgctct gatgccgaaa
      661 agcgccgtgg tgtttttcaa caggtcgtgc gtgtagtaca ggttttccac ccaggttccc
      721 gtctcatcaa agaggctcat ggccaggatg cgcgtggctg ccggttccag cggatcccac
      781 agataggttt ttcgcagtac gggcatcgct gattccgtcg cggcggcatc cagttccgcg
      841 atttgcaggt agccgtgata gatgaaccgt ttcttcgaca tcaggatctc tccttcatag
      901 acggctttct caatccgtct gttcagatag tcgtagcggc actccacccg cctgttcccc
      961 tgaatgaacc ttgccgcctg gttcagggca ttataagaga cttcccattc tcccgtagac
     1021 gtttgaatct tcgtctggtt gccgtcggca tcataggcag gtgcaaaaga cgctccttcc
     1081 tcccgggtga tggccgtgta ctggttcaga ttattggccg tgtacgcctc cgctgacgcg
     1141 cccgaacctt cccgagaggt gacgcggttg ccgatgttgt cgtacgcata ggaatacgtt
     1201 cctccccggc tcatcgcgtc ggcggccagt tctctgcggt cgttgtaact gtagctgtgc
     1261 gtcaggccgg gagcaggagt attgaagtaa tccttctttt ccgtgggcct tcccagtgcg
     1321 tcgtaggcat agtcgttttt ggctggataa ttggcgctgc cgggacgcag atagtcgatt
     1381 ctggtcagca gattccgttt ttcttcccgg gtgtaccatc tccggagggt attggggtag
     1441 tcgagcgttt ccagcagtcc gttgacggcg tggtagccat agacgaaggg atcggcgccg
     1501 ttattgagcg ataccgtaga aagacgtccg cagccgtcat attcccaggc cgtctgctgg
     1561 acaataccct ctccataatg cagactgtag cccgaaggcc gccccaaacc gtcgcgcaaa
     1621 tagttgagcg cgcttgccgt cagtcctgcc gtagcttccg attccgcttc attatattgg
     1681 ttgtaaacca acgtccttgt tccggaatca tccgcaacct gagtgagcag gttcaggtgg
     1741 ttgtagacat aattgagcga aggagtcgca tcgttgtgaa tgacggagac caggttgccc
     1801 gtctgcggat cataggcata agaggttgcc agaggcgttc cctgcccgtc caccgtccgg
     1861 gcctgtcgtt taaccgccag cctgttccag tcatcgtaag agtagctttc cccatggccg
     1921 tcggcatagg ttttagccgt catcaggccg gaggagtcgt cgtagctcca cgtcgtcatg
     1981 tccccgtccg tccgttcccg cggatcggca gcgatggttt cctccggaac gcggaatgtc
     2041 atgagggata ccagcctgtc cgcatcatcg tacgcaaaga cggagggctg gagggccgta
     2101 ccgtattcgg cggttttgcg ccctcgcggg tcataagcgt agcaggccgt gttgcccaaa
     2161 gcatccgtga cgcaggaggg agaagccgtg gacgggtcat actggatggt ggtcgtattg
     2221 ccggctgcat ccgtcctggc tgtttcacgg ccggcgatgt caaagacggt aacggcggca
     2281 ttgccgcggg cgtccgtata agtctccgtg ctgccgctgg ccgtataagc gcgggaagag
     2341 gcggtcagaa cacccgcatg gtctttccgg gatacggtgt acccgtctat gacgacagtt
     2401 tccgcaatga cggaagaagc cggggactgc gtttttaccg tccgtctggc cggagccgtg
     2461 tattccgtcc attgcagggt ttcattgccc cgcggatcgg tggagatgtt ttttccggcc
     2521 agcgaggagg acagggaaga aaccagctcg gccgtgcttt ccgcatatgt tgttccctgg
     2581 ctgttgcagc gcgtggctgt ggttacccgg tacacgccgt cttcccgttg ccggcgggca
     2641 taggcgtact ccgtgacgag ggagttggcg ggagtggggt tctcggccag cttgagcgtg
     2701 gttttgacga tattgccaaa cgggtcatag tcataaagca aaggagccat tccatccacc
     2761 tgctgccgca aaagcctgtt gttcagatca aaaacgcgcc ggtcatgaac aaggcctccg
     2821 ttggcgttcg cccgggagac gcgcaccatg ttgtcccttc cgtccgtgac ggtttgttcc
     2881 accagctccg gttctccctc cgcccgggga agaagcgtgg aacgaaccac gccttccgcg
     2941 gagtattccg tacggtagag gagatgcctc tgccccgtgc cgctttgttc cagcacggtt
     3001 ccgtccgcat ggcggatagt gacgagcgtc gctcccgtcg gcgtggtgac ggtttccgtc
     3061 aggccgtccg cactgtaggc acgcgtactg ctccttccta agacatccgt ttcctgcacc
     3121 agctgcccca gcagatcgta aaccttgctt tccgtcgtcg tcatgggacc gacgtcccgg
     3181 cgcgtttgca ggattctgcc gaaggcgtcc cgcttgtagc tgacgatggt ttcaggagtg
     3241 acgaccgttt ctccgtccgc gacttccgac cggctgactt ccaccagctg gcgcgccgta
     3301 ttataggagt aggtggtcat gatgccgtct tcatcctttt cccacaaggg gccgcagcac
     3361 atcatctccc gttccgtcac tctgccgttg ccgcgcgtac gcttaatcca ccggttttcc
     3421 ctgtcgtatt cgtaatccgc cgtatccgtc agcgtccagg ttccatccgt cagcaaggca
     3481 tatttctcaa tgcgcgtgtt atttccctga gcggaaacgt acgtgacttt cctcgtgctg
     3541 tgccccggta cggcctgccc cgctatctgc gtttccctgg tctccctgta gagggcgccg
     3601 tactggctgg ccgcttcata gccatagacc gtctgcacgc cgctggcgga ctgcttcatc
     3661 ttcagcctcc cccgggcata ttcgttgggc gccgccggca gccacgtttc cgtcacttcc
     3721 agctgttctc cctccgcacc caacgccgtg gtgcgttttt ccacacggcg cacatggttg
     3781 gcttccgtat aggtgtaatg cgtatccgcc agccgtgcgg cgttcatagt gagcaccacg
     3841 tactggtgcg caatatccgg atcggcatgg tcggagcagc ggtaataggt gtaggtgccc
     3901 ttccttcccc cgcccgccca gggttccatc cggacggttt cacgcccgaa ggcgtcgtag
     3961 gaccaggtct tctcgcttcc gtcgggagcc gtctcccgga tcagtttgcc cacggcgtta
     4021 taagcgtaag tcgtttcacg ggcatagtcg gttccataag cctcaatacg gctggtgcac
     4081 nnnnnnnnnn tgccgctgcg gccctgttca taggtttcgc acacccggga aataggagtg
     4141 ccgttttccc cgcgctgcac ggtagtgaag agtttccaaa tccccgaaga aacctcctgc
     4201 ctttcacgga tcgtgaaaat agagtcttcc ccttccccct gggacatgca ccaggcccct
     4261 cccgtcccct gccagtagcg ggtgacgtaa ggcgcgcgcc cttccgcctg ctccgtgacg
     4321 gtgagcttgc cggtctccgt attgccctca atggtaaagg ttttaaaagg cgtccccgta
     4381 acggcgtaaa gaccggtgga gacgtttttg gcccccacct gctcgggcag ataaaaggcg
     4441 atcacatagc ccgtatccgt cacgttttca atattggcca gaccgtccca caggttccag
     4501 acctggcgga tgacatcatc cgcgcttcgg acgatgtcca ggtaattggc aaaatcctga
     4561 gccgtccaag aagaacccag cttggtccgg tagccggagg ccgcacccaa agcggaaacg
     4621 gaagaaccgc cgtagatgac tgtattgccc ctggcgttgc tgaccctcat ttccgccgcg
     4681 ctggcggaag ccgaaacggc acgcccctct gccgcggcat acgccatgga aacggaaccc
     4741 gtccttttcc gcgagccgcc gatggagcct gcactggccg cgccggcata gcagtaatag
     4801 ttggcgcggg tgcctccgtt ccggatctgg aatgccatgt cagcccccag ctcccggccg
     4861 gaagggggca gaatacagct ggccatggga tgcaggtatt gcagggctgc gggcgtccac
     4921 aacctggaag agaagttata agccacgatc tccagcatcc cgtacggcac gccttccata
     4981 ccgcggaagg aaccgaagct ggtgcgccag accatgctgg ttttccgcgt ctgggcagtc
     5041 acccgtttgc ctgcggaaga gaactttcca taggcggagg cggaggaaac caggctgcgc
     5101 acggcagacg cgccggggga accgccccgg ttgtccatgc agtcatccgg ggaaaaggga
     5161 ttgtccggat caatctcatc ctcatctccg ccgttgtcgc cattcgggca cggattgctg
     5221 gaactgctgt tgggagaact gctgcttcca ctgctggagc ctccgcatgg atcatcatcg
     5281 ccctcgtcgt tgttgtccac tggctccgtt tccgtctccg ggggcacata ggaagaagaa
     5341 gaacccgttt gccggttggt aatttccacc ttcaggtcaa aggtgaattt ggcaatgttg
     5401 ttattggcat tgggaatgga aatattgcta tagtccacat gtgcccgatg cattccgggc
     5461 tcaatgctga aagaagccgt ctgcggttcg tagctgccgc ctccgtactg tccgcgcggg
     5521 ccaatgtcca gccgttcttc tccatccacg gtcaagttag ccagatcgtc ggcgcccatg
     5581 gtcaccttgc attcggccgt ttcatcaggc cccagctccc tgacttcaaa atcccagtcc
     5641 cagcttcttt caaaagcgtc cgttggcgct tgttcgtcga tcgcggaggg agctccgcta
     5701 ttggaattgc tcatgcccgt cagggaattg acatcgttat caaaggattg ttctttcatg
     5761 gtttgaatcg gattagctat tgaaacgcaa acattgcatc atcccaatct tggaaaaact
     5821 gcaataattt ttatccgttc cttatccata acaacaaaac ctcctgtttc aacacgtcca
     5881 acacgggatg ctcctggcct tagccgtgtt caatatatgg tgacatttct tctctgcgca
     5941 cttccgaatg gaaaactctg gcccatatgg aaaattccgg aagtagcata tccaaatggc
     6001 ctttaactgc acgccatgaa acacagcaat ttttcgttta acatgaattc atgaaatctt
     6061 caacagaaaa agaatgatct ccctgaattg aaaaagaaat ccaaaatcca taaaaacggc
     6121 tatcgccttc gggcatgttc agcatccctg acatatccga acggaaagaa agcaggataa
     6181 actacccaca tccgagcatc cattcctgaa ttttacaagg aaagctgctc cttcaaacgc
     6241 tccagcagat tgccgcaacc gtcctgcagc aattttacca catgctcaaa gccttcctgt
     6301 cccccataat agggatccgg tacttcttca tccggaaaac gagtcgtgaa aaaacacatg
     6361 ggcactattt tgcctgtcca ttgtcccttt ttatccaatc gtttcaaatc ccgcagattt
     6421 tcacggtcca ttcccacaat caggtcaaat tgttccaaat cctctttccg cacggggcgc
     6481 gacttaagtc ccggatcctc atatccgtat tttttcagtg ccgtcagcat ccggctatcc
     6541 ggagggcagc cccgatgata accgatcatt ccggcggaat cgctctcaac caaatgttcc
     6601 aaaccttgtt ctgcgaccat ttttttgaaa atgatttcag cggcaggaga gcggcagatg
     6661 ttgcccagac agacaaacag gactcggtaa ggttcgggag cattcatgga cgtcatttct
     6721 ttttcaggat aggcaggcgg cagccccatt tcaagaggga agagtttatg catgggaaac
     6781 ggaaacgccc acgaatattg catggggcag agaaaggaac tgtgccgctc attcaaaaat
     6841 aagattctcc tgtgtttcgg cttgcgtcag aggatgtccg gctgtagatt atcgcatggc
     6901 agatttccct cttggattaa gttttgatga cgtgctcctg ctgccccgcc tgagcgcgat
     6961 tcttcccggc gatgcggaca tcagttccca gcttgttccc ggctttgaca tgaaaatccc
     7021 cgtgttgtcc gcggccatgg ataccgtttc cgaatcggaa ctggcgattg ccctggcgcg
     7081 ggaaggcggc ctggccgtca tccaccgcaa caaccccatt gacatccagg cggccatggt
     7141 ttcccgcgtg aagcgttttg aaaacgcggt catcccgaac cccgtcacgg tcaacaagga
     7201 catgacgctg gaagaagtcc accagatcat gatggatcaa ggttactccg gcttccccgt
     7261 cgtggatgcc aaccgcaagc tggaaggcat catcaccggc cgcgacatgc gcggcgtgga
     7321 cgattaccag aatgtccggg tcaaggacgt aatgactccc ctttcccgcc tgattacggc
     7381 agctcccacc acgaccattg aggaagcgcg ccacatcctt tacacccacc gcattgaaaa
     7441 gcttcctttg gtggatgaac acggcgtgct ggccggcctt atcacggaaa cggacattca
     7501 gaagcgcgcc atgtttgcag acgcttccaa ggatgaacac ggccacctgc gctgcggcgc
     7561 cgccgtgggt gtcggccccg attatctgga ccgagccaaa gccctgattt ccgccggagc
     7621 ggacgccctg ttcattgacg ccgccacagg acacacgacc cgcgtgatgg acgtggtttc
     7681 caacctccgc aagctgacgg accgtcccat cgtggccggc aacgtggtta cggcggaagg
     7741 ggcagccgat ctcatcaagg cgggcgtgca ggccatcaag gtgggcgtgg gccccggctc
     7801 catttgcact acccgtgtca tctccggcgt gggcatgccc caattcaccg ccattcagga
     7861 ggtagcctcc gtggcgcgtc ccgcgggcgt caccgtcatt gcggacggcg gcatccgcta
     7921 ttccggagac attgtcaaag ccctggccgc aggcgccgac ctggtgatgc tgggcggact
     7981 gctggcaggt acggaagaaa gccccggcaa ggtggtccat taccaaggcc gccacttcaa
     8041 gcagtatcgc ggcatgggct ccctgggcgc catgcgccgc ggctccggcg accgctacgg
     8101 tcagaacagc tccggcaagc tcgtcgcgga aggcgtggaa gcgcgcgttc cgtataaagg
     8161 catgctggcg gacgtggtat tccagctcat gggcggcctg cgctccggca tgggttatct
     8221 gggcgcacac aatctggagg aactccggaa caaggcccgg ttcgttcaaa tcacctccgg
     8281 cgggctgaag gaaagccatc cccacgatat caccatcacg gaagaacccg tcaattattc
     8341 ctgttaactc ctccctcaca tggacgacaa gcacctcgta gccgtcattg acttcggctc
     8401 ccaatacacc cagctcatcg tacgccgcgt gcgcgaactg ggctacatgg ccaagctgta
     8461 tgcgctggaa gacctggacc agattcacga acccggcgcc gtcatccttt ccggcggccc
     8521 caaaagcacc acggatgcgg acgctccgga cattgacttt gaatggctcc aaagcctgaa
     8581 tgtccccgtc ctgggcgtgt gctacggcat gcagctgctg aacatcaagc acggcggcac
     8641 cgtaaaagcc agcaacaagc gggaatacgg ccccgccgcc ctgctgccgg aaacctgcgc
     8701 gggcctgtac cgggacatgt ccccttcctc ccaggtatgg atgagccatt cggacacggt
     8761 ggaccatctg gcggaaggct gccgcgtaat cgcccgcaat gcggaaggcg tccccgtctc
     8821 cctccaatgg ggagaaacca ccttcggcat ccaatttcat ccggaagtga cccattccca
     8881 tgaagggcgc accatcctgc gcaacttcct ttcctgtgcg gccaatctta aaaagttcga
     8941 catcggagac tttaaaaggg aactcatccg ggaaatccgg gaacgcgtag gcaacaggca
     9001 ggtggtctgc ggcgtctccg gcggcgtgga cagcaccgtt ctggccgttc tgttgcatga
     9061 agcgggcgtg aacatgcgcg ccatctttgt ggataacggt ctgctccgca aaaacgaggc
     9121 agaggaagtg cgggccaatt tcgcccgtat gaacgtggaa attgaaacgg tggatgcttc
     9181 cgaacgcttc ctggcggctc tggacggaga aagcgatccg gaaaagaaac gccgcattat
     9241 cggaggcctg ttcatcgacg ttttctggga tgccgtggga gacgcggaaa tgctcgctca
     9301 gggcaccctg tacccggacg tgatcgaaag cgcctccaac gccaaatcca aggcctccgt
     9361 catcaagacc caccacaatc gcgtggaacg cgtgctggaa ctccaagcgc aggggaaagt
     9421 actggaaccc ttggcggaac tgttcaagga cgaggtgcgg gaactgggcg cctccatggg
     9481 catcccgcat gatattctgt ggcgccaccc cttccccggc cccggcctgg ccgtgcgctg
     9541 ccccggcgta gtcaccaaag aacgcctgga catcatccgg gaatgcgatg ccattttcat
     9601 cggcaatctg aaaaaatacg gctggtacga caaagtctgg caggcctatg ccggcctcat
     9661 ccccgtaaaa acggtgggag tgaaggggga cgaacgctcc tatgaatggg ctaccaacct
     9721 gcgcgccatc gtgtcggaag acgccatgac ggcggactgg gtagaactcc ccccggccct
     9781 gctgagggaa accagcaacc gcatcctcaa tgaagtaaag ggcatcaacc gcgtccttta
     9841 cgacatttcc accaagcccc cggcttccat tgagtgggag taaagcttcc gcaccctgtt
     9901 gacggccgtt cagcagggtg cctccgctcc tcctctccgg taaacaaaaa tcaagaaagg
     9961 agaaaataat gactgaactg gaaaaatgca tggcgggcga atggtacgac tgccatgaca
    10021 aggtgtttct ggaattcaaa aacaagacgc accggctcct gatgaaatac aattccctgc
    10081 cttacgacca taaggaggaa aagcgccagg tgctcaagga aatgctgggc agcgtcggca
    10141 ccaaggtctc catcggtcat tccttcatct gcgattacgg ccgcaacatc catatcggga
    10201 acaacgtcac cgtcaatacg ggctgcacgt tcgtggactg caacaggatc accattggca
    10261 acaatgtgct gatcgccccc aacgtacaga tttacaccgc tacacacccc attgagctga
    10321 atgaacgcct cactcccgtg gaaacggatg aaggaatcga atacatccgc catacctacg
    10381 ctcttcccgt aacgatagaa gacggttgct ggattggcgg cggcgtcatt atcctgcccg
    10441 gcattaccat cggaaaagga agcgtcatcg gagccggcag cgtggtcacc aaaaatattc
    10501 ctcccgacag cctggcggcg gggaacccct gcaaggttat tcgtaaaatc aacggaagtc
    10561 gggaacaatg atcaagctga tcgcttttga tctggacgga accattgggg acacggtccc
    10621 catgtgcatc agggcatttg aaaaagcagt ttctccctat gccggacaca cgctgagcga
    10681 acgggaaatc acgcagactt tcggcctgaa tgaagtagga atgatcaaga aagttgctgg
    10741 agaaaaatgg cgggaagctc tccatgattt ctacccggtt tatgaagaaa tgcatcaatc
    10801 atgcccgtct ccgtatgaag gtatacgtga attgataaaa accttgcaaa aggctggcgt
    10861 gcttgtcgcc ctgattaccg ggaagggaga caaaagctgc cgcatcacgc ttgagcaatt
    10921 cggcatgcgg aatttgttct gttccatcaa gaccggaacg gaagacaaac caaacaaggc
    10981 ggaagccatt gaagaacttc tgcatgactt ccgcttgaac aaagatgaat tttactatat
    11041 cggggatgcg ggttcggatg tggccgcctg caacaaagcg ggggtgacct gcctgtccgc
    11101 cgcctgggca acgaccgcag atgtcagcgg attgaaaaaa gccaacccct caaaggtttt
    11161 tcccagcata aaggaattga cattattcct gtccattgag aaataattag aagatactcc
    11221 tcacttggat aaggaaaaca tgatggaaaa atttcaaaac ctggcccttc taattgatgc
    11281 tgaaaacatt gctctggata agcttgaaag cgtcattcag aaaacatctg catatggccg
    11341 tatcgtgatt caaagagcct acggcgactg gagcaaggaa agtttaaaaa actggaaaga
    11401 ctcgctaaac cggtttggga taaaagcgca gcagcaattt aattatactc ccaaaaaaaa
    11461 ctcaacggat atggcgttga ctattgacgc tttggattta ttgtatcaat catcttacga
    11521 tgcccttgcc attgtttcga gcgactgcga tttcactccc gtttccatat atgtcaggga
    11581 acatggtatt tatgtaatcg gtatcggaga gaagaaaact ccagaggctt tcaaaaaaag
    11641 ctgcaataaa tttattttat taaaaactgc tgactctaaa acagataata cactacaaaa
    11701 taaatcttct caatctggat cagaaaaacg gaaaaaaatc cctgagtctg ttcatagatt
    11761 acttaaggag gcatttgaaa tacacaacaa agaaggatgg gtagctttat ctcgggcagc
    11821 atcttacatt aaagaaaaac agcccgattt ttctttaaaa gaatattatt ctcaatttta
    11881 taaactcatt cagaattttc aggaaaaata cacacttgat aaatcacaca caaaatacct
    11941 atgcaaataa aattgtagaa ttaccttcta taagtctatt aacctgatcc tatcctggga
    12001 caggaaataa attcctgttt tttatacact catttaacgc aaaaaagcct ctgttgccat
    12061 ggataccggt gccgtcatcc tcacctcctt tctctccacc attcccgttt atcttttctt
    12121 tgccacgggt ttctatctgc gccacaagca agtcatccag gcagaccacg acgcgcccat
    12181 catgcgcatc gcgatggatg tggcctatcc ctgcctcgtc ttccacagta tcatgaagta
    12241 catggtgctc tccggaaatg agacgctcag cagcgtttcc ttttccctcc aggccattgc
    12301 agccggggcc ctggaacttc tgctgggcat cgcggctgca tggctggtcg ctaaactgct
    12361 gcgcatgcgc atcggcacgg gccttcgtac gttcaccctg acggcgggag tgcagaacta
    12421 cgcttttttc gtcattccca tcatccagat gctgtttacc gccggcaacg accccacgct
    12481 gggcatcctt ttcgtccaca atgtagggtg tgaactggtc gtctggagca tcggcgttat
    12541 cattatcgca ggcggtccgg gcaacctgaa catgggcgtc tttttccgcg ggcccctgct
    12601 ggccgtcatc gtgggtctgg cgctcgcctg gtccggcttg ggaacctatg tggcccaacc
    12661 gcccctgatg aagacttttg aaatgatcgg caactgcgcc acgcccctgt gcctgatcct
    12721 gttcggatgt tccatgcggg atttatggca taacatgaag tgggaaccca aacccatcat
    12781 ctgcggcctg ctgacgcggc tggggctggc ccctgcactg ctgctcctga tggcttattt
    12841 ccttccggtg gatgattata tcaagcgcgt catcatcatc caggcagcca ttccctccgc
    12901 cgtcattccg gtcattctgg ccaaacgttt cggaggccat ccggacctgg gcactcaaat
    12961 cctgctgaca acgacggtgg cttccttcct cacactgccc tgctggctgg ctcttggttc
    13021 caccctggtc gtaccattgt attaacggga taatggcgac atcaatcccc ggatgacctg
    13081 gaacgtcccg cttgcccaac atcttccgct tttccggagg attccattcc cggtgtcctg
    13141 ccccttgatg gaaataaaac cagtctgcaa ggttcggcgg cggaacaatt tccctgctga
    13201 aatatccgca tcctttccgt ctccatcctg cattccatgc tgaatgcttc cctacctttg
    13261 ggttgactcc ccagtcggat atgtattgaa tggattcatg aaatttcctg ctcctctctg
    13321 cgccgccctg ctgccggtcc tgttttcctg cgcggagcag gtttcccgga ttgaacacgt
    13381 tcgggaggtg cccttaaaaa ccacggtggt tcctgcggaa gacgtaggca tgcaccgcac
    13441 ccaggccacc tatctgctgc atggcgccgt ctcccagcaa cagcgccttc agcgtctggg
    13501 tcaatactac ttcgtcacat ggaacgatgc caatccggaa caggcggcaa ccctggtcat
    13561 gatgtaccgc cagggaaaga caggttcaac cgtcctgaca aaatccattt cctatccagc
    13621 cgggagaaaa ggcggcacgc aaaactccgt tttcgaattc atcggggatg aggccagaga
    13681 aaaaggggaa gtcctcgcat ggaagctcgt cctgaaggac aaccgcggca aaacgatttc
    13741 cgaacgccat tcttatctgt ggggggacga tcattgactc ttcggcaaaa acaccgaaga
    13801 gccgtaaaat cacccggata tacaggatga gacctgcagt ttccggcagt ctcgccagcc
    13861 tccaactccg ttaataatcc tccggaatat gggcacgctt acgcttcctg ccgtttggca
    13921 cggcgtttgt ggacagcgtc ggcaagggtt tccagcactt ccactgtcgt ctcccaccca
    13981 atgcactggt ccgtgatgct cttgccgtac accaagtcgg aactcaacgg ctgggctcct
    14041 tccacaatgt tgctttcaat catcacggca acaacctgtt cggacccgct ggatatctgt
    14101 tccgcgatat tggcggccac agtcggctgt ttgcgataat ccttgcaact gtttccgtgg
    14161 gagcagtcta tcatgatgcg gttgttgatg ccggccttct gcaaggcttc ttccgcttcc
    14221 tttacatgat cttcatcaaa gttcggcccc agggaggaac cgcgcaggat caggtggcag
    14281 gacttattgc ccgtagtgga aacaatggcg gaaactccct gcttggtcac ggaaaggaag
    14341 cagtgcgggc aggaggagga aacaatggcg tctacagcga tttgcaggct gccgctggtg
    14401 ccattcttga agcccacagg catggaaagc ccggaagcca gttcacggtg tacctggctt
    14461 tccgtggtgc gcgcgccgat ggcgccccac gtgatcaggt ccgcaatgta ctgcggcgtg
    14521 atggtatcca ggaactcggt agctgcgggc actcccatat cccccaggcg cagcagcagc
    14581 ccgcgggcca tatggaggcc gcggttgatg tcaaaggtat ggttcatgaa cgggtcgttg
    14641 atgaggccct tccagcccac ggtggtgcgg ggcttttcaa aatacacgcg catgatgatc
    14701 accagatcct tttcaaagcg cgccatttgt tccttcaggc gggaggcata gtccacggca
    14761 gcctggggat cctggatgga gcatggcccc accaccacca gcaggcggtc gtcgtggccg
    14821 gaaatgatgt tctccgcatt cttgcgggtc gtagccacca ttttggcgat ctccgtggtt
    14881 gccggataat ccttgattaa aatggcgggg gaaatcaggg gctcaatgtc ttgaatgcgt
    14941 atgtcgtctg ttttgaacca gttcatgtct ggggcttatt gagaggtttt tgagagaagt
    15001 tgaaaagaaa aaaatacggt ttgcgggctt tatgcctgaa tataccgcgg ggcctcacgg
    15061 ccataagccg gaagccccgc aaaatgaagc ggccgcgcct cccgacaaga ggcgccgtca
    15121 tgcattacca agaagtcaca ttgtcttccg tcaattccgg attgttgata tggccgtccg
    15181 ccgtgctgcc ctgcttgtcc ttgccggtgc tgtaaacggc cacgttcttg gtcaccatcc
    15241 gggaacctac ctgaacacgg ggagtacggt cgtcagggtt ggcattcaaa atgatgtaat
    15301 agggagattc ccaaggatcc atcaggccca cacggccgcg gctttcaaaa aacaggccgg
    15361 gagcgccttc cacatccgta gtcgtcgtat tgaaataagc cgttttcttg ggattcaact
    15421 ttctgtcctc atccgcccgg tccatataat tggtcaggat gctcatgatg cggctatcgt
    15481 cgtccacggc agttgtcacg taatactgtc cgtccttgtg ggcctgcttg gtcatttccc
    15541 ggctgctcca ggccacggga acgcggccgt tgtccagcat gtaattttcc acagccgtct
    15601 gcaggccgtt gcagtcgctt cgcgccgtgg taaccttgga tttttccatc acgccgttca
    15661 ccaccgtcat ggcgatgccg gccagcgtca ggataatggc aatcaccaca agcaattcaa
    15721 tcaacgtgaa tcccttggac ttacggttca aaaaaggtgt cttcatactt tgccaagagt
    15781 agcgcaatta cggttcaggg caagcggaaa acaaccggcg ccgcctattt caaagcctgt
    15841 tcccggaatg gcagcagacg gaataacccc ggtgcacaaa caccccggcg ccttccggca
    15901 gccggggtgt ttaaaaagaa tgctccttta gttggagggg acaggggttt ctgcggaagc
    15961 aggggccgga gcctgagaag aagcggcttc cgcctcagcg gcgcgcctgc gcagctcctc
    16021 ctgctggagt tcttccggcg tcttggcggg aatggaagct tcctgcctgg cggcagcctt
    16081 catcatttcc gggctcaaca ggctttccga actcttaacg tgctgccggt tgatcagcat
    16141 gcccagcccc aagcaaagaa ccatgaacgc ggttccgaag tacaccgtgg cttttttcag
    16201 cacatccgtc gtccgggcgc caaaagcctg gtccgtaatg gctgcgccaa aggcggctcc
    16261 cagcccttcc tgcttgggcc tctgcataag cactacgccc agaaggagaa gtgatacaat
    16321 caccaaaacg gcaaaaagta actgaatgct gatattgagt gaatctgcta aaaaaaggtt
    16381 catgagcgcg gggagtatgt gtgaattcga acggtttgta aagtctagga aatgatttcg
    16441 ttatgctcgt tgcagacgat tttcttgggg acgatgggct tgtccgacaa ggcaaaactc
    16501 ataatgacca cccgttcgcc cgtcttgatt aaatgggccg caccgccgtt catgatgatg
    16561 ttccccgtgc cggcaggccc ctggagagca tatgtttcca gacggttccc ggacgtgacg
    16621 gacgccacga gaactttttc cccgggccac aaatccacgg cggccatcaa atcggcagga
    16681 atttcaatac tgccttcata ttgaacatcg ccacgggtaa tcatggcgcg atgaattttt
    16741 gattttaact gctcaaccaa catgacgttc gaacaacaaa cccccgatcg cccctccggt
    16801 caagccgtca tcctccattt tacggttttc tgtgtcttac tccccctcct ccagcgcttc
    16861 cgcggcatgg gcggcatccc gggcaagctg gcttttcaat tcttccaaag aggggaattt
    16921 gatttccggt cttaggaaac ggaccggttc cacttccatc tcccgcccgt aaatatcccc
    16981 cgggtggccg aataaatgcg tttccagcaa aagttccccc gtctcacctt ccacggtggg
    17041 gcgcaccccc agattggcta cgcctccgta ttttttgccg gccacccggg cgcgcacggc
    17101 gtaaacgcca tggggaggct gtaacccgca accgggtctt atattggccg tggggtagtc
    17161 cagcaggcgc cccagttggc gcccgtgaat caccgggccc ttccagcggt agggacggcc
    17221 aagcatgccg gcaacctgta aaaaatccgc cccgcgcacg gcttcgcgaa tccgggtgct
    17281 gcttatgcgc tccccgtcct gcagaatagg aggaacggct gtcacccgga acccgtagcg
    17341 gcaggacagc aggcgcaggg tttctacatt ccctgccctg ccgcggccaa aatgccagtc
    17401 ctctcccacg gcaatctgag ccacccggca ggaggaacaa agttccccaa taaactcttc
    17461 cgggctcatg gacgccagcc tggaagaaaa ttccagcgac agcaccgcct tcacccccag
    17521 ctcttccatc aaggcaaatt tttcagcatc ttcccccatg atggcgggcg gcacgctttc
    17581 cggcctcacc cgggccagcg gatgggtgcg gaatgtcagc acggcgggaa ttcctccgtc
    17641 aagagcagcc cctttcaccg ccgcggcaat cactccggca tgtcccaaat gcaccccgtc
    17701 aaacatcccc atggcccagt gtacgggggc ggcaaggctt ttcaattcct tgaattcccg
    17761 gtaaatgacc atatgctacc cggcaatcag aatatctgcc agctccttca gggaaagcat
    17821 ggcccggaac agttcctccc tgggcgcggt tttcagggtg tccaccgtaa tggcgcggtc
    17881 cagcgtaaaa gaaccggacc tggtgcgccg gagggcgctc aaatgcgctc cgcagcccaa
    17941 tgcctttcca atatcataag cataggatcg aacataaaaa cctttggaac attgaacggt
    18001 aaaatccact tccggaatgg ccacgcgtga aattccgtgg ccaaacactt ccacaggccg
    18061 cggctcccgc acaatttcct gacctttccg ggccagtttg tacagcggca ccccgtcctt
    18121 tttgatggcg gaaaccatcg gaggaatttg ttcaaaagct ccgtcaaacc ggtcaaacgc
    18181 ttcacgcacg gcctgttccg tcactccgtc tacggaatac tccgccacta tttctcccat
    18241 ggcatcctga gaggaagtct ccacgcccag cataagggtt ccggcgtatt ccttgtgttc
    18301 gcacatcaac ttgtcctgaa gcttggtcgc cttccctacc accagcatca gcaatcccgt
    18361 agccatggga tccagcgtgc cgcagtggcc tattttctta attcccaggg aacggcgcgc
    18421 aattgcgacc acatcgtggg aagtcatgtc ccgcgccttg tcaatcaaca ggacgcccga
    18481 aggaacctgg atggaatctg aaacattcat gaaattttct gtcggagcac gcgttccact
    18541 tcctcccgca tggccttcag gatagtaagg cgcacttctt caataggccc ccggatacgc
    18601 actccggcgg ccatggaatg gccgcctccg ccgaaacggg aggccagctc cgcgacggat
    18661 atttcaggcg tcttggatct cagggaaata cggatgcggg gatcgtcctc cgtttcttcc
    18721 agataagcgg ccagccagac tccctgcaca gaacgcagaa ggtcaatcag gccttccgta
    18781 tcttccggac ggcttccaat ccgggctttt gcctcattcg tcaggcaaaa cgtgcaaatc
    18841 ctgccgtccg gcgtcagctt cattccgttc agcgcttcac gcatcaactg cattttcacc
    18901 cacggctttt cctggtaaag ctggcggttg atgtcctgga cgtcaacacc catccggatc
    18961 aaatccgcag ccagttccat gacggccgcc gtcgtcatct ggtactggaa agaccccgta
    19021 tccgtgctga cggccacgta aagggcgtct cgcatcaccg gagtgacggg aacgcccaga
    19081 tacttgaaaa agtaatataa aaccgccccc gtggcgcatt cgtccggcaa aacgcactga
    19141 acgtctccaa acagctcgtt cgtccgatgg tggtcgatgt tcgcccacac cttcactccc
    19201 gccaaagcct ccaggctgcg ttctcccagt ctcttgagcg cgccgttatc cacggaaatg
    19261 gctacttcca catccaccgg ctcatcaggc gtgggaataa tattttccac acccggcata
    19321 aaggccagat tggaaggcac cccgtcctcg ttcatcatca ccaccttctt tcccatttgc
    19381 ttcagggctt cccccaaagc cagcgtggag ccgatagcgt cgccgtccgg atgaacatgc
    19441 gcaattaccg caaaactgcc gtggctgcgg atcagatccg ccaaaggcgc gaattgggga
    19501 caatgtttcc aatcttccat atgcactgcc gtgtttttat agaaaaagcc gcacctggac
    19561 aatcaccaag tgcggcttga aaagtcgtac ttaatcccgg aagaggatta cttcttggac
    19621 ttcatgacca ccggagccac atccacggcg ggctggttca ggtcattgaa cttgggcagg
    19681 ggggtcaggg gcggagcacc ctgatcctgg cagcagcagg aagccatgat gctcgccgca
    19741 aaagcggcaa gagccataac ggtaatcttc ttcatatgcg tggttaaatg atgggtgata
    19801 aaggatatgg aaatatcttg gagcataaaa gcataccatg cgggcgcccg tcaatcaaat
    19861 ttgcgaaaat ctcccaagga gacgcctttc ccaaaatggc aagacgcata aatcgctcgc
    19921 ccggggttat ctgtaattat aatctctgcc aagctgtatc cttttcatgc ccttttccgt
    19981 caaaatactc cggtttttcg gcattctcct gctgagcctc tgcgcgctcg gcgtggccgg
    20041 catgacctgg gcgtacctgg tagaacccaa tcttctgttt gtcaggaaag tggactaccg
    20101 tgtcccccag tggaaaggga agggaacacc cgttaaagtc gttattgccg gcgatttgca
    20161 cctgatgccc accgcgtttg atgaagtgag ggccctgcgc tatattcaga ggatcatgcg
    20221 gctccggccg gacctgattc tgctggttgg agactatgcc cggggaagca gcgaaaatgc
    20281 cagcatggat cccgctacgg ccgcccggct gctgaaaggc ctgacggctc cctgcggggt
    20341 cttcgctatc cagggaaacc atgatttcac cttcggctgg aacaactgga gcagggaact
    20401 gaaacaggcg ggaatcaccg tgctggaaga ccagagctct cttgtcacgt tcaaggacgg
    20461 aaggcagctc cagctttccg gaatattgga tacgacgcgc aatgtcagaa aacggctgcc
    20521 caaacgccag tcgccagaca tcccccattt cctgctttcc caccgtccgg aaacggacca
    20581 catactttcc catggagacg tggacatcgt catcagcggg cacacccacg gtggccaggt
    20641 ttgccttcct ttcggcctcc tgcggtttga agccagcccg gaccatgccc taccctacac
    20701 ctatccctgg catatctccg gcggcaatac ctacctgatc accaagggac tggggacaag
    20761 cacactcccc ctccgcttca actgcccgcc ggaaatctat ctgctggaac tgcattgacc
    20821 cattcctccc gttaaaaatg cagtcccttc cttcccggtt ccctccccct ccgctccacg
    20881 ccggcggcaa tccataagga aaacggaaaa gaacggccgg gttccatagt ttcaaaggag
    20941 ccggttatca aacccttcct tcagccttaa aatctttctg gccgtcatcc ggtaaacagg
    21001 ccggtaaagg tgctttttca agacggccgg gagcgttctt ccgggcagcg gaccgcgcag
    21061 cagcccggct tcacgcataa cccgttcgta acgttcccct tcataacgcc aattataccg
    21121 ccagggcttg tgcaccccct ggtaatgaag aatgcccggt tccaaagccg cttccaccgc
    21181 ctggcgcggc gtaacgccgc gccagaactg ttcccgggga tcgtttttca aaatgcggcg
    21241 cgtcaagccg tcatgccagt tccagcgcgg gtgcagaggc acagtcagct cgcacagggc
    21301 tccgttcaag gcatcctggt ccggacatgt cagcctgtcc cggtgagtga cggcataatc
    21361 cagcacagcc ctgaccagat tcctctcccg gaagacatcc acattcatca gcagcacgcc
    21421 ggaattgaaa tatcccgtgc aggtcagcgg catctcaaga cgttcgttaa aatgggaacc
    21481 gggtctggaa aaattctcaa acaccacccc tactgctttt ccgtccatat tcgtccggaa
    21541 cagctccgtc aaatcccggc aaaccagaac gtcaatgtcc agatacagaa tattgccccg
    21601 ctcttccttc aataattccg gaatgaacac gcgtccccag gctggcaccg gccattgttc
    21661 cgtatggggg aagtcgtgct cttccaaaat gccggaaacg tctatgaact ccagacggca
    21721 atcgaacgga gcggccagcc gctccacgct cgcccggttc tctccgtcaa tgccgtcgga
    21781 aagaacgtaa attttgtaaa aagtctccgg accggcagta ttcagcaaag aaaagacagt
    21841 aacgctcaaa ggcagaatgc ccctgttgtc actggccagg acgacggcaa attcattctt
    21901 tttcatcgga ttattcattc gggtgatgtc agcggcgcag caaccgggct cccgccttgc
    21961 gcattttctt ccagtaatag gaaaagtcca agccggattt tccgcccatc caggctttca
    22021 gcaattttcc ttcccggcgg gacaggcagg catcatcctc tgcgggacgg tacagatttt
    22081 cctgacggag catatcagtc accttccggg taacctcctt ttggacggca tgaggggcat
    22141 atttgcggat gttcctcacg cccattacca gcattttcaa aaatgccgtc ctgtattcct
    22201 caagaatccc ggcttctttc cattccttca gcaccagttc cgccaccgtc aggtaatcca
    22261 gtgactggag ggaactgccg gacgcaaaaa cgctggtaat ggatccggcc cgcttgcggt
    22321 aaacgtaagc cgccctgctg acgacgcaaa ccctcctgca ccatggaaaa acggcgtaat
    22381 ggaaatacag gtcttcacag gcaacgccct cggggaaccg gatgccatgc ttttccacaa
    22441 tggaacgcct gatcatcatg ccgcccgctc catgatgtat tttcccaatg gtctccggag
    22501 tgcagggaag ccaccctttt tccggagggg ccatgtcctc cggcagaaac cggtcctccc
    22561 ggtcgggggc ggactcgtaa taataccgga acccgcatgc cagcctgtcg gcatccagcg
    22621 ccttcgcacg gcggtacatc tcctccagca aatccgtttc cagccagtcg tcggaatcca
    22681 ccagaaacag atattctccc acagcatggc tcatcgccag attccgggag cgggataggc
    22741 cgccgttttc cggagcctgc acgaccttca cccgcgggtc ccgctccgcg aattcccgca
    22801 gaatggacag ggagccgtcc gtggaagcgt catccacgca aactatttcc agctcacgga
    22861 gcgtctgtga gcaaatgctt tccaggcact gggccaggta cggctccaca ttgtaaaccg
    22921 gaaccagcac ggaaatgttc acgggagccg tcatgagcca tgcggagaat ctgccgccct
    22981 tatttgttca aatccttcct gcggcggcag aaagggctcc agcgcggacc atacctggtc
    23041 cactgtaatt tcctcaatgg cgccggggga aaccaggttg gaaatgcacc gggcagtaat
    23101 ggcggaattg cgcgtccggt ggcagaaaat ggcggtcacg ggcaccccgg cataggcaca
    23161 catatgtgta ggccccgtat cattgcctac acaagccagg gaacgcaaag ccatctgggg
    23221 aatatccatc agggaagact tgcccagaaa actgacagcc aggggagagc tggcggcaat
    23281 ggcctccact tccgcagcct ccgcccgggt gccgatgaca acggaggaca cgccccgcgc
    23341 cgccaggcgt ttcgccaggg cgcagaaatt ctccacgggc cagcgcttgt agggatggtt
    23401 gggggaacag cccggaatca tcatcacata gcgttccgga agctcgtcaa aatgcagtcc
    23461 ctccccgtgc atgaaagaaa gatccgtcag gacgcgttcc aaacggaacg gctcccgttc
    23521 caccttcccc caccggaagg atttcttttt cagcaatttg cgccgttctc cggaattgca
    23581 ggccacccag tccatgtcat ggttcagcag gaagcgcacg gcgggataat agcgtttcct
    23641 ggtgcgggaa gattcctgaa gatcgtacac cacgtcaaaa ttccctttca caatggaacg
    23701 gacggccctc caggtggcgc ccagcttcca gtaaggcagg cgattatcca caataaaatc
    23761 gtcaaacact ccgagctgct ccgccatggg aacaaacatt cccatggtca tcagggtgat
    23821 atgcgcctcc ggatgaagtt cgcggagccg cttcatcgtc cccatggcga gcaccacatc
    23881 gccaagggct ccatgcttga tgatcagaat gcgctgtgcg ggcataggag aaaaaaggaa
    23941 tgcgggttaa acgtaactgt cttccctgtc cagatactcc tgcacgtacc ggcgcacgcc
    24001 atcctccagg gaagtcatgg gcgctgcata accggcaacg cgcagcctgg aaaggtccgc
    24061 ctgcgtataa tactgatatt tccccctgag ctcttcaggc atctcccggt aggcaatctt
    24121 ttcctgaagg cccagcgcat ggaatgccgc cttcgccaga tcgtggaaag agcgtgcctt
    24181 cccggaacct acgttgaaca ggccgctcac ctccggatgc tccagcagcc aaagcaccac
    24241 atccacgcag tcgcccaccc acacgaaatc ccggagctgc catccgtcac ggtagtcggg
    24301 atggtaagat ttgaacagct tcaccgtttc attggccttc acttggggga atatatgggc
    24361 caccacgctt ttctgcccgc ccttatggta ttcattaggg ccgtacacgt tgaaaaattt
    24421 aagcccggcg tattgggggg gaaccggacg cccttctttc acttcccggg ccaccttgcg
    24481 gtcaaacagg gccttgctcc agccgtatgc gttcaggggg cgcagcgcgt tgacgtgctc
    24541 cagggaggcg tcatcgtcaa accccatgga accgtccccg tacgtggccg cggaggaggc
    24601 gtaaataaac cgtttgccgt acagggaaca aaactcccac aaatgccagc tcagcgtgaa
    24661 attcgtccgg ataatcaaat cggcgtccgt ctccgtggtg gcggaaatgg cccccatgtg
    24721 aatgaccgcc tccacctcat ccgcatgcgc cttcatataa tcaagcatgc cttccggaga
    24781 aaccacggcg cacagctctc tcttggcaat gttcctccac ttgtcggaag agcccagctc
    24841 gtccaccacc acaagatcct ttttaccggc tttttccaga gcggcgacaa tgtttgagcc
    24901 gataaacccg gccccacccg tgacaataat cataagaaaa gaatcaataa aaaagttgta
    24961 cggccttttg taccacatcc acccgtccct aggcaactgc catttaaggc gttccccggc
    25021 tcccaatttt caacgaattt acaatccgga cactccaaac cctgaccgtt ccatgcggaa
    25081 aaccattggg ccgcaatccc cgcgcagcct gcggagggtg cgcccctcaa atcctctttt
    25141 catactggca acgttaaaaa atttcggtaa gacctgtctt gcaaaaatta tcattgacca
    25201 cggattgtaa atccgttaca cacattccca cccctatgat ccccgatgtt tccatcattg
    25261 ttccctgcta caatgtagcc gcttacgtgg acagctgcct ggagagcctg gtacgccaga
    25321 ctctccggaa catagaaatt atctgcatca atgacggctc cacggatgag acctggacac
    25381 atctgctgcg ctggaaagaa aaggacagca gaatcatcct tctgaaccag cggaacgcgg
    25441 gcgtctccgc agcaaggaat gcagggctgg atgccgcccg cggcctttat gtcggttttg
    25501 cagatccgga cgactacatg gatccggaaa tgtattcccg ccttttttcg gcggctctgg
    25561 aatatgacgc agacatcgtg gaatgcggca accatgtttt tgaagactcg tcagaccggc
    25621 ttatcgaagc caaaagaaga tcaccctccc ggcattttga agagaacgcc tctccggcca
    25681 gcttcttccg ggattccatc tgggggaaaa tggatatctg cgtgtggagc aaactgttcc
    25741 ggaaaagcat gctggacgcc caccgcctcc gcttcaacgt acatctgaaa tccggcgcgg
    25801 aggatgaaac cttccggctg atggccgttc cccatgcctc ccgtctcctg ttcattcccg
    25861 actgcctgta ttactaccgc cttatgcgca acggctccct ctcccgccgc tgcaacgttc
    25921 ccacctactc caaatgcgtg caggaattcc agcggttgct gtacattgtg gactactggc
    25981 agaaacaagg atggcagaat gaaggcctgt tcgcttacgg cgtccggaaa atcaggcctt
    26041 tttttgtttc caagcatccc cttttccatc agatgaccgc tgtccagcaa cgttccgcgc
    26101 tggactggtg gagcctgttc tatcagaagg cggaaggaaa acgtttcctc tccgctctat
    26161 cgggacggga caggcaactg gcggacctgt tgaactcggc ggaaccggtc cccaacggct
    26221 gggggcgcat tctgctggca gcctgctccc tgcttcccgg acaaaaagga cgttattact
    26281 cctgcaaaaa aatgcttgcg gaacattttt cccaagtctg ccccaatggg tttcaaaaag
    26341 aaaacgcttc tctggaagag ccgtttgaca catcgccgcc ttccctgtaa attgccttat
    26401 cctcccattc cggatcaatg aaccgttttc tctcatttgc agactcccgc atgtcggccg
    26461 ccctggagcg gctgggcagg caggcggaag cgctggattt ttttgatgag atcacgctct
    26521 tcacggaaca cgacctttcc gcagaattca ccagccgcat gggccggtac ctgacgccgt
    26581 cctgccgggg attcggatac tggtcctgga aaccctgggc catccaccat gtcctccaag
    26641 aaatggagga aggcgaccgc ctcctttatc tggatgcagg ctgccatatc aacgccaacg
    26701 gaacggagcg cttccgggaa tacgtaaata tgctggaccg cgacagctgc ggcatgctgg
    26761 ttttcaccaa cggacagccg gaatacaaat ggaccaaagg agatatcttc cgccacttcg
    26821 gcgtttccca agaagacggg catatcacgc atacccagca aattgcgggc ggccatgttt
    26881 tcctgaaaaa aaacccgctc acggaaagcc tgatccggga ttggctccat gtcttttacg
    26941 accatctgca tcttgcggac aacactcctt ccgcctcccc caacctgccg ggttttgtgg
    27001 aaaaccggta tgaccagagc atcttttcca tcctatgcaa actgaggggc atcaccaccc
    27061 tcgacggatc ggaaacctat gcggaaaact gggaacagct ctccttgttt ccattccagg
    27121 acagacggga catgggaatc ccacgggccg ggaaaaaggg atggctggaa aaatgcaggc
    27181 agctccttct ttcttccaaa agaaacggat taggaatccg gtaaagcttc cccattcgga
    27241 acaaccgggc tgcacgacgc ttccaaacgg ggcaatctga cgcaccatga actgttttcc
    27301 cgtcctattc cacagcttca tcccgaaaat tcctttaccc ttcggctgcc gtttccggaa
    27361 tgtaatgacg gttatttgga caagctcttt tcatttgatc aggaatgttt tgccttcatg
    27421 aaagcaaaca tccctccatt ccaaaactcc gcacccgcac tatcctccgc cgacattctc
    27481 ccacccctac tcacagttat gccgtcaccg gaatccggct ttcccccatt cataccggaa
    27541 tgaaaataaa aaggccatca tgctgggaac agcctgccgc agttcatgga atactggcag
    27601 cctggatttc cccaaaacgt ttttctttca gatttgtagg cttgacggcg gattcctaat
    27661 cctcatactt tcctatgtgg cttctcctca atgccgaaca atatttcaag tctcccatag
    27721 cgtctcggcc agctccgccc cttaccggtt gagcgacgct ctgaaccggc atattcccgg
    27781 cctgaaatcc accctgctgc tccggaggcg caaagaaatt gacggcctgg aaatccacag
    27841 gacacccgcc agaagggcta cggacgcctt gcgccgccat ctggcccatg gatttgacat
    27901 tctgctgccg gaaagaagaa aagacctgcc tttctccctg aatgtgctgg gcatgggatt
    27961 tgacacggcc caaatggaaa aagccgatgt agtgcatctg cactggatag gcggcagaac
    28021 cgtggatttc tcgcagctgt gccatatccg ccgtccattg gtctataccc tgcacgacat
    28081 gtttgcctgc actgcgggat gccactgcac catggactgc ggcctgttcc aggacgaatg
    28141 ccgcggcaac tgcccccagc ttggcgctcc ccgcctcttc cgcaacattc cagcgtggct
    28201 gttttcccgc aaacgccgcg ccttcggccg catcccgtcc cttaccctcg taactccctc
    28261 cctctggtta aaaagggaag tggaaagaag ctgcatgttc cctggaagaa aaatcgtaca
    28321 gatttacaat cctgtggaca cggacctttt ccgtccggtg gaagaccgga atgccatccg
    28381 ggccggccta ggcatcccgc ccggagcgtt cgtcatcgcc tgcggcgcaa ccggcctgtt
    28441 caatccggtc aaaggcggcc atttcatccc cctggtgctg gaggaactat accggcgcgg
    28501 gcaccggaac cttcatctcc tgctcttcgg caaccaggaa aaaagcgtgg actacccctt
    28561 ccccagcaca aactccggat tcattacgga catgaacaag ctggccgggc tttacagcgc
    28621 ggcggacatt ttcctgaacc ccacgcttca ggatgtcctt tccaacgtag cgctggaatc
    28681 catggcctgc aaaacccctt ccgttacctt ccggacggga ggagttccgg aagcggttct
    28741 ggacggaagg acggggctgg tcgctccgca gggggacctg aaccaaatgg ccgcccactg
    28801 cgaacggctc atccaggacg gaaaactcct gcaaaccctg gcggaggaag gccgtcggca
    28861 cgcggaacaa acattctcct accccgtcat tgccgcacgg cacgccgccc tgtatgagga
    28921 aacgctccgg gaataccgtt acgaagagtg aaaacggccg gcgttcaacc gccggactgc
    28981 ctgttccagc acatccgggc gcatcttcct ttgtcattga caagaaaaag acgcctcata
    29041 cactctgcac cgtttcaacc cttctccccc catgaaaacg cttaaaatct cctttttaca
    29101 gtccactccg gatttcggca gggaaggaat gcttcagctt ctgaaaagcc ggtatcatgt
    29161 ggtggaagac gattccgatt ttgattacct gatcgccacc ccctggtttt acgtcaaccg
    29221 ggaggctttt tacgattttc tggaacgggc ccccggccac atcaccgtca tgtacggttg
    29281 tcatgaagcc attgcaccgg atttcatgct gtttgactat tacatcgggc tcgatacggt
    29341 gcccggaagc gaccgcactg tcaaacttcc ctaccttcgc caccatttgc aggaagtgca
    29401 cggcggtaag gaaggcctgg atgcccatgc tctcctggcc tccaaaacgg gtttctgcaa
    29461 cttcatttac gccaaccgca aatcccatcc caacagggat gccatgttcc acaaactctc
    29521 cgctttccgg ttcgtcaatt ctctggggcc ccatcttaac aacaccccgg gcgacggcca
    29581 ccgggcggaa gactggtacg catcctccat cagaatgaaa aagccgtata aattctccat
    29641 tgccttcgaa aatgcatggt accccggcta caccagtgaa aaaatcgtca ccagcatgct
    29701 tgcgggcacc attcccattt actggggaaa tccggacatc agccgggaat tcaattccgc
    29761 ctcattcatc aactgccacg acttcccaac cctggacgac gccgcggcct atgtgaaaaa
    29821 agtggacgaa gacgacaacc tgtggtgtga aatcatgtcg cgcccctgga aaaccccgga
    29881 acaggaagcc cgctttctgg aagagacgga aagggaaacg gccaagcttt acaaaatatt
    29941 cgaccaatct ccggaagaag cgcgccgcaa aggtgacgga acatgggtat cctactacca
    30001 gcgcttttta aaacgcggcc acaggatgca gctggcatgg cggcggctga aaaaccgcct
    30061 gcgccgctaa ccaacgtttc cgggcaattc aaccccatgc cggatttcct ctgtccgcct
    30121 tcccccgtcc ccatccactt ccttccgctt catgagcaaa ttcttttctg aaaccttttg
    30181 ttcaccttct ccccaccaag ccggaggcgc catccacgga acctatttcc cgcacattga
    30241 cggcctgcgt acttttgccg tgctggccgt cgtcctgtac catttgcagg aagggatctg
    30301 ccctggaggc tatgcaggcg tggacatctt ctttgtcatc tccggatatc tgattggcgg
    30361 aggcctcatc cgcagcctga aggagggaac gttctccctg acctcctttt acctccggag
    30421 aatcaggcgc atcatgcccg cctacttctg cctgattgcg gccgtgctgg ctttcggctg
    30481 cgtcgttctg gactgcgatg acctgcggac tctgggccgg acggtgagat ccagcgccct
    30541 tttcatcacc aacacttact tcagcagaac cacaggggat tacttcagcc cggcggcgga
    30601 agaaaaccct ttgctgaacc tgtggtccct gagcgtggaa gaacaatttt acctgatgat
    30661 tccgcttacc ctgtggctgc tctggaaatt caaggaaaaa gccgtcaaac ccgttctatg
    30721 gggggcgctg ggcctctcct tcttcgccgc cgccttccac atggggcatc tggaacataa
    30781 caaggccttc tatctgcttt actgccgcgc ctgggaactt ctggccggct gccttctggc
    30841 tctggctccc gccgcagaac agaaccgggg aaccggatgg ctgcggacgg cgggatgggc
    30901 cggaatcctg ctcccctttg cctgttatac ctcttccact ccttttcccg gatacacggc
    30961 catcccttcc atcgcggggg cggccctgct tatccgttac ggaaaccatg gctggtccgg
    31021 gcgtatcctc aggcacccgc tcagcacggg catcggcaaa atctcctact ccctctattt
    31081 gtggcattgg ccagttatcg tgtactggac atacgtctgt ttcgatgaat gctctccctg
    31141 ggattatgcg ggcatgttcc ttctctccct tcttctgggt ttcctttcct ggaaattcgt
    31201 ggaaaccccc ttcagaacga ccgtctcatg gcaaactccc ggaaaagcgt ttctggccac
    31261 ggcagcaggg tgcctcatgc tgggatttgc cggtgaatgg ctcaaatgga cggacggcgc
    31321 gcgggattac tggcatgtag cggccaacag catggaattc cctgaatact ggaaagggcc
    31381 ggccttcccg ccctccttcg gcatcacgcc tcccagccgg gcggtggtgg aaaagggcaa
    31441 cgccattcag caccatcatc cggagctggg ggatgtgacg gattaccctt ttgtcctgct
    31501 gggaaaaaac gggcaggccc cttccttcct gcttatggga gacagccatg ccatggccag
    31561 ttcccccgga tttgacgagg ccgcgcggct tcttcagcgc tcgggcctgt tctaccgcgc
    31621 gcgcctgtgt ccgctgagcg gcatctccgg ctccggagac tcctcttccc tgacgctcct
    31681 gcgcctgatg tacccccatt gggaacacaa tatggaacta attctggact ggctggaaaa
    31741 taccccccag gtaaagaccg tattcatcca caaccgctgg attgaactgg tgaacaacca
    31801 ccgggatacg cgcctgaaac aactggttgc agacggcctg cgccacacct gtgcgcgtct
    31861 tcggaaagcc ggcaagcaaa tcgtgctgct gggtcccgtt cccgaatgga ctttcggccc
    31921 gcgcaagctc atgcggcgca atgccctgct gaacgccaac cggacagacc gcctgctggg
    31981 cggggacttc gacacgcggc agcgtccggt attcgccctg ttggagcaca tggaatccac
    32041 aggcctgtgc cgcttcattc ccctccacgg caccttccat aaaaatgggc ggtggctggc
    32101 ggaagacggc ggccaattaa tgtactgcga cgacaatcac ctttccccct acgggtccag
    32161 aaaaatggtc ggcgacatca tcgaccagct tttcccggga atggaaagaa cagcggccaa
    32221 ctgacggaag ctccgcacgg gaaaacatcc aatcctttga cattgacaag gcatgcaggc
    32281 cccctacact ggcgccaggc agcaaagcct tcgcgatggt cccgaataaa agcccggaat
    32341 atggaggcca taccgcgccc ccctcttcaa cgcctgtact cccatgacgg attcctcccg
    32401 ccccacacgc atcaaggtca tcagaaaatc catgaaggcc tctccagatc aaacggaatc
    32461 cctgttgtgg ggcgactgcc tgttctgcac ggacgccgcc atgacggatt acgactgggt
    32521 tctggtatac gacgaatttc ccagaactcc cgtgggcacc atcaggaatg aaacggaacc
    32581 ccttctctgt cctccggacc agactatcct gattacggtg gagcccccct ccatcaaaat
    32641 ctacagcagg gcatacacca gccaattcgg caccgttctc accacccact ccgtccggga
    32701 ccttccgcac cccgggcaca ccttgggccg cggatgtctt gaatggctgt acatcaagcc
    32761 catgcaggaa attctggacc agaaggaatt cccgaagaca aaaatgctct ccaccatctg
    32821 ctccgccaag cagcatacgc ataccatgca taaaaagcgc tatgatctta cgcgttacct
    32881 ggcggaccgt ctgccggaac tggactggtt cggacacggc atccgggaaa taaaaaataa
    32941 aaccgtcgcc atggatgact acaaatatca tctatgcgtg gaaaaccatc tggaacccca
    33001 ccattggacg gaaaaactgt ctgatgcctt tgttgccatg acccttccct tttacgccgg
    33061 ggatccgttg gcaacggaat gcttcccgca ggaaagcttc atccccatcc cgctggacaa
    33121 cccgcaaaaa gcccttgaaa tcatccgcaa ggccatggag gacggagaat atgaaaaacg
    33181 gctccccgcc atccgtgagg caaggcgcct ggtgctggaa aaatacaaca tgttcgctca
    33241 aacagcggca gtcattcaca accacaggga aacgggaacc atacgccccg gagccatgct
    33301 gaaaggacgc cacgttctgc ggaaaaatcc gctccatgcc cttcgggaat tgacggacac
    33361 gctggcctat aaaatcaggt cgcgcggcag gcgcggaacc ggaaccggag cataacctgg
    33421 attccgggcg cacggcagcg ggggggaggg ggcaggcctt ccctgccagt aaagaaaatg
    33481 ggcgtccgat cttctgccga tggccgcgcg gctggaggaa cggaaagcct ccatgtgacg
    33541 gaaagattca aaaggaacgc tttccccggt acccagcctc ctgcccatta aagcatgaac
    33601 ggctccgtca accctcttct cagggaatac aggacctgga accctttgaa cgacagggaa
    33661 accgcaaccg tatccgccat tcctccgcca caccgcaacc agggcggcaa ataccgtccc
    33721 gacagccccg tccggtctga aatcgattca aaccggacgg aaaacaggca attaataaaa
    33781 cggaaaaacc ttaatctgct ttgcgggaag cgtgcctctt ggcccgttcc tcctcccgct
    33841 gcttcttcag gtcttccttc atcttggcct tctcttcgga atccagcttg ccgtccttgt
    33901 taacatcata tttttcaagg agggcggctt tcttttccgc agaagtcatt ctcttgggag
    33961 cgtcttcctc cgccatggaa ggcatggcga aagcaacggc cagaacaata agcaaatact
    34021 tcatgattaa acggggttct tgaataatcc ttttctactc ccgcgcgtcc cggttgtctc
    34081 tcttttttta gcaatacatg caaattcccc ggccatgccc cttttctgcc gcgtgaactc
    34141 cccccggtta agccttcccg aacgggaaaa atagcggaat caccactacc atgaccagcc
    34201 ccatgatcac ctgcagggga acgcccactt tcacgtaatc catgaacgta tatttccctg
    34261 ccggcataac cagggcattg ggcggcgtgg aaaagggtga agcaaaacac atgctggtgc
    34321 ctacggaaac cgccagcagc agcggatagg ggctgatgcc catactcgcc gcggattgca
    34381 gggcgatagg cgccagaagc accgccgtag ccgtattgct gataaacatg gtcagcaggg
    34441 acgccgtgaa atagacgccc gccatcaggg caaacggccc gaatcccccc agtccggcca
    34501 ccagcccgtg ggaaatggcc tctgaggctc ccgtcttctc cagcgctacg gacatcggca
    34561 gcatggcggc aatcagcacg atgctctccc agttgatcgt ccggtacgcc tcctccacat
    34621 tgcgcagaca gccgcacaac accatcagga cggcggcaat caataccgcc gccacagcag
    34681 gcacccagtt gaacaccatg gccgcaatca tcagcagcat aatgcctgcg gccagggggg
    34741 ccttatgcgt cagggtcacc ttggaggcct ccgccagagg ttggcccacg acaacccatt
    34801 caaaagtttt ctcgctcagg cgcgcaatat ccgtccaggt tccctgaatc agcagcgtat
    34861 ctccgtactg gagcttcaca tccttcaaat tatggaaaat gtaatggttg tcccgctgga
    34921 tggccagaat attgaccccg tacttggcgc gcagccccga agacttcacg ggcatgttga
    34981 taaggtccga gttccgcatg agcagcattt ccgcaatgcc tatctcgtca aacttcaatt
    35041 tgcttttcaa cagggaatcc gtatcttccg acgtcttccg gttaatcatc tccgccccat
    35101 attcccgggc aaaacgggcc acgtcctcaa actcccccat gacataaatg acgtcgcccc
    35161 gggccaccct cgtatctgaa cccgccatgg tctggctgac cgtctgcagg aaggaatgcc
    35221 gtccggaatg cctgcggcgc acctccacaa tattcacgcg gtaaaggtcc ggtatctgaa
    35281 gatcatgcag aggatgttcc gtcagagggg aatcctccag cacccggagg cggtaaagat
    35341 tattaataat ctggtactcg tctatcaact ggctgaggga tttccccttg gcctcgtgtt
    35401 ccgccctgtc gtcatggcgt cccagaaacc tcctgctgag ggggatcagc accaggatgc
    35461 ccgtcacgat gcagacaagg ccgatggggg tgaacgcaaa aaagcccagc ccctccattc
    35521 cggcctcctg aagctggttg ttgacgacaa ggttgggggg cgtgccgatc agcgtcagca
    35581 tgccgcccat actgctggca aatgccatgg gcatgagcag acggctcgtc tggatgcctc
    35641 cgtccgcagc caggctcacc agaataggca gcatcagcgc taccgtcccc gtattgctga
    35701 caaatgcgcc tatgccggct gtaacaagca tggtcagcac cagcaggcgc aactcgctcc
    35761 tgccggccag tttcatgatt ctgccgccca tcatcttggc caggcccgtc cggaaaatgg
    35821 cgcctcccac cacgaaaagc cccaccatca tcaccacaat ggaattggaa aagccggaaa
    35881 gcgcctgttc cgggctcaaa atcccgaaca taatcaggca gagcagggaa cacagcgcga
    35941 caagatcaga acgaatcctc ccctggacaa ataaaacggc ggatactccc agaatgagaa
    36001 gcgtggtcaa catagcaata aacggcagga gtgaaaaaac ggcaagggag gatatctaac
    36061 atgacctgca cgtcttgtcg ataggctgaa caagtcattc acaaaaccgt ctctccgccg
    36121 gcatccggca gaaaaaacac tttatgcatc ctgctcggag aaccccgctt cctctttcct
    36181 tctccggtat catggggatg aggaaacaat aaaaaacgcg ctttctgaaa gaacgcgcca
    36241 taaaatgaaa agtggtgggt agagaaggat tcgaaccttc gaaagcaaat gctaacagat
    36301 ttacagtctg ccccctttga ccgctcggga atctacccac ttgaagtgat gtgcggcgaa
    36361 tgtataggga ttcccgtgcc ttagcaagtt ttttttataa aaagtaggtc ctcccagaag
    36421 cagaaacccc atatgcaaca gccatgccgc cggaaggaaa gcggatgcag gaacacaagg
    36481 ccggaaaagg caattttcac gaatctccca catataccgg agcgcggctg tccggggaat
    36541 ccgcatccag ttgctccagc tggggcagaa tgttctccat gctctcccat acggagggaa
    36601 caatcttgat tgcggttcca aaccggactg ccatcatcag ggcgtctgaa gggcgggcgt
    36661 ccagctccac cagcttgtcc atcacctcat tggcggcttc tatggtcacg cgggcataaa
    36721 agacttcatc cttgcggccg ttgataagca ccagcctcac ccggcattcc atggcggata
    36781 aaaagtgagc cagcagggaa gacgtgtccg gccgtaccgg gcgttccccg ttcaggcagc
    36841 gttccagggc catgccgtct gtcagctcca tgaaaatgac gcccaccttg ccggtcagag
    36901 gatcacgcag gcacacaccc gccccggccc gggtgtaaag cagggcataa ggttccagag
    36961 caatcaaatt ttcatccggc atggcgggcg gaaatcacat gttaaaaacc gggattaata
    37021 acccgttccg tatagcgggc agccaccttg acgtattttt ccgcccattt gtggttatta
    37081 tccgcggatt ccggccccag gtccttcacc accttggcag gagaacccag caccagggaa
    37141 ttggggggaa tgacggtacc tttggtaatg agcgcgcccg ctcccaccac agacccctgg
    37201 ccgatgaccg ctccatccag aataatggac cccatgccta tcaggcaatt atcctccacc
    37261 gtgcaggcgt gcagaatcac gccgtgcccc acggttacgt acttgcctac atagcagccg
    37321 aaatcgtctg ccaggtggat gcagctgttg tcctggatat tggagtaatc accaatgacg
    37381 atcttattga tatctgcgcg cagcgtggta tggtaccaga cactggcatg ttccccaatg
    37441 gtaacatctc caatcaaatc agcgctttcc gccacaaaag cggtatccgc cactttgggc
    37501 cagaagttcg cgtaaggttc tcttgccata taagtcatgt attaattgaa gataagacag
    37561 cattccagtc taaaaacgtt cattccgcgg gtatgccgca gccggaaacg aacacggccg
    37621 aacccacccg aaaatgaaag gccgcaggca cctccgcact aattttcttc ccggatgccc
    37681 ttcttgctgt tcttctcccg cttctccgca tccttgcgca gggagtcggc ttttttccca
    37741 ttacctcccc tttcataata cccggctatc ctccgctgga tggcggcttc cttctttacc
    37801 ttgaaataat cgcccgattt agtccggata taccggttgt aggaagattc cgcctccttg
    37861 gccatctgaa gcacggcctc ctgatcctgg gaaacgtact cgtaatattg ccccatcagc
    37921 acttcaaata cttccgcgcg gcgcgcataa tccttcatgg acttccggta cagggcagcc
    37981 accccggcct tgtccccgtt ttccatcagg agatccgcct gccgcttgat ggcggcggaa
    38041 gtcaggtcgc tgcgcccctc gtccgtctcc ttttccagct tcttcaaatc gcgggcaaca
    38101 tggcgtttat tggtggaagc gtccctcatg gggaaaatat actcatcttc cacgcgggcg
    38161 gccatggcca ggtaatccgg acgggaacgg aacttgcgct taatcatggc caccagttca
    38221 tcccattctt ccagcttcgt gccgctctcc gggcggccca tcagagcaat caggcgttcc
    38281 cagcccaggg ggctgccggg agtggcctgt gtggcggctt ccagcattac ccgggcgcaa
    38341 tccggcttct gcatctcctc aaacaggtcc gccagggaaa gataatccag ggaagtatcc
    38401 agccgggcgc cattcacctt cttgtccata ccctggagaa gggtggattc atgctttgac
    38461 ttgcagttgt gcggatggga aaaatgcccc gtattatatc cgtacccgcc gtaggactgc
    38521 caggtttcat ccgtcgtcag cagattgacc caggcatgcg gcccgcggtt gccgtccccg
    38581 gtaacatagg cggagggaat gccgttgcat ttggcggtat tcaccgcaaa ataggcctgg
    38641 tccatgcaaa tgccgccttc atccagaatc tcttggaagg tataattagt ataagggtcc
    38701 ttgcccagtg cggcgcggtc catccggtaa cggatcatgg aataggctcc gccccatcct
    38761 ttccgggtaa ggcgcacctt tttgcgcgcc caatccatct ccgacctcgg cagacgcaca
    38821 tccaccacaa acaacagctc ggaagggctt aatttggaaa tgtccgtgag aagctcacgg
    38881 gcttcatcca tctccatgaa gtactcgaat acctgttcca gcgtcaaagg cgtggtggaa
    38941 atattggtgg aagaagacgc cctcagcttg gacgggctgg aagcgatgcg gggattcagc
    39001 atggcgcagg ccagcgccag ggaggaatac ctgctccggg gcatggcgga ggttttcatc
    39061 cagaattccg cccacttcct caaaatccct ccccactcct ccccgggtgt ctgctcccgc
    39121 gccatcgtgc gcaggaaaag tctcagggga tcggaaggat ctgaaaaaag ccatggatag
    39181 aaatacctgg gggaactctc cttgatttgt tcctggatgg tttccgctcc cactttgctg
    39241 atgagctggt acagctcatt ggcgcgcatg aggtacgggc tctgatcgta catggacgcg
    39301 tcaaatttgc cgaatttgat gaccttcacg gcatcccgct ccaaattgga gcgcaggaaa
    39361 tcggcataca agtcatagcg gcccgtttcc agggccagat tgaaagcatc cgtaaacaac
    39421 tttctgttgg cggcgttatt ggccttcagg ctgaccagaa gaacaggctt cttgaaaatc
    39481 tccagccgcg gatcttcctc ctggaccacg ggttcttcct tcggttcctc aggggaaggc
    39541 tccgccactt tcgattcctt gttctgccgt tttccggcgg cttcctcagc agccaggcgt
    39601 tccgcctcaa gacgggcttt ttcctccgcc tccgcgcgcg cccgttcctc cgccttcctg
    39661 cgcgccgcct ccagttccgc ggctttccgc gcacgcgccg ctttcgcttc cgcctccttt
    39721 ctggccgcca gctcagcggc ggcagcctta cgttgaacgg aaacccggtg gtaagccacc
    39781 cctccggcgg caagagccag caccaggaca atagtcataa taattccgcc ggaagaggat
    39841 tttttatgct tgggaaaacc gggacgcaga ggattgacag gattcatggg gatgcagctt
    39901 gaacgcgttg aagcaagttg gggaggggga tgagacggtt ttatccctgt tccgtgtgac
    39961 ataaaagcgc aatacctttc caaggtcaac ttgaaacaat ggtcttttgc tgaaggttcg
    40021 ttttcccgga acccgttaag aatcaaacca gagtattttt ccttgtattt tcatagccat
    40081 tttatagtat caggcctctt catgtttcca aaaacatgag aaaaacacaa gtatcctgct
    40141 gattattcct ggctgacata tgtttttccc gttgcctatg aacactttct ctcatctcag
    40201 tccgtttctg gccgtcattt cgggaagcct cttctccgga acggccctct gtaacgacat
    40261 cgtgctgcct ccgccagata aaaagggagg aaagcccctc atgcaggttc ttcatgaacg
    40321 gcattccacc aggtcctttg cagacaaacc tatcccggtg gaaatcctgt cctccctgct
    40381 ctgggcggcg caaggcgtca accgggacga tcccgactac cgcacggcgc cgtcttcccg
    40441 gaattccaat gaaatagaaa tctatgtcgt cctgccgcag ggcacctatc tttacaggcc
    40501 ggaaacccac cggctggaga aagtggttca gggcgacatg cgggccgcta cgggaacaca
    40561 ggaactcgcg gctaccgcgc cgttaaacct ggtctatgta gtagaccagt ccaaacagcc
    40621 gggagacttt gatgccagga gaaagctggt cacggcctgc acggatgcgg ggtttattgg
    40681 agaaaacgtt taccttttct gtgcttccga agnnnnnnnn nngctcggca ccgtgttccg
    40741 cgccatgatt aacgccaatt atatccagca gtgcctgaag ctccccgtcc tcaaaaaagt
    40801 cctgtacgcc cagagtgtcg gctatccgtc aaaatcctga aaaaaccata agccggaacg
    40861 ctgtgagact cgccgttcca tcggcggctc ttcctcatgc ctgcgaaaaa ccgtcatggg
    40921 aaccgccgtt cccatggaaa taaaaacgcc aggaaccatt gccgcgccag ccaggagcgc
    40981 acttttccgg acagcccggc aacaaaggaa aaagcgtttg acgaactgtt ggatgaacgt
    41041 ttctggcaag cccggttccg ccactccgcc ctgcggcatt ggctggagaa atattcaaac
    41101 cggaattccc gggaaactgc ggcgccagga aatctactcc ccgcttgcgg tctttcccat
    41161 agttcaaatt catggaaaat ccggaaccgc aagcggaaaa cgcaccagca ttaccactgg
    41221 ttgtccaggt ttttggtatt tatctttccg ggttccttgt tgcgttcctt catgccgcgg
    41281 acaggcttgg gctccttttc gtgcaggtcc agaatatcaa tcacattctc cccttttttc
    41341 aggaatgggg ccggaacata cagccttaac tgaggtccca cattccaata gcgccccacg
    41401 ttgattccgt tcacccatac ataccccttg acatagcggg acatatccag aaacgtgtcc
    41461 tgaggctctt caatatttac aacggcccgg aaatgcgccc cttcccgttt ctgggaaggc
    41521 ccctttcctt tgggagcccg cacaatggaa tcagctttca ggggaagcgc cctcaccgtc
    41581 cagttcttta actcccgcgt tcccagcttc acaggaccgt aaatcccctt gcggtcactc
    41641 tccatgctga tatgaaaatt aatatgtccc atggcctcta ccagaacttc cagcgtcccc
    41701 ggcttcctgc gctccggcag tttcacactc ttctgcccca gccgccgatc cagcgtgccg
    41761 accaactctc catccagata cacctgggca tagtcattga cattggtaaa ttccagcgtt
    41821 tcaggcggtc ctgacggaac ggccgttctg taaacggcaa taccctggtt ctggttccat
    41881 tgttcaaaat agggaggctg cgggaacttg gaccggaaag gcttgggcag gttctcccaa
    41941 aggccggcat gcactgcggg cgtgaactcc ggaatttcca tggccggaat gtccgcggga
    42001 ggttccggca cagcggcttc gggaggcaac tttttcaaaa tgatttccct catctggaca
    42061 taatattcat tcatccgccc ctgctcatcc acgggagaac cgtaatcata actcgtcagg
    42121 tccggctggt attttcccgg ccctccgttg ttggctccgg ccgagaatcc gaaattggtg
    42181 cctccgtgga aaacgaacaa gctgaaggaa cgccccttgt ccataaacca gcggacgtgg
    42241 ttgaccactc caggggtggg agcccaattc ccctcccccc agtgccgcag ccagcccgga
    42301 tacgtttccg aggaaaaaac gggaactccc ggattgcatt tattagccac tgcccaatgg
    42361 ccgtcgttca gccccggatc cagccctacg gccacgcctg gaagcaccac gcctttcaga
    42421 aaatgttctc ccgcgccgtc ggaggtgtaa aaggggccaa cacccttcct gctccagaac
    42481 gccttcagcc attccatata cttgcggtct ttacgctggt agctcccgta ttcgttttca
    42541 agctgggtca tcagaatggg gcccccgttt ttggctaaaa aaggctccgc cacacgagcc
    42601 acggcctcca gataacgcgt ctgcgccttc atgaatttgg cgtcttccgt agttctcaac
    42661 ttggccttgg gatccttcag caaataatga ggcagcccac ccagatccca ttccccgcat
    42721 gcataggggc cggggcggaa taaaacccac atgccttcct cctggcataa cttgaggaac
    42781 ccttccagat cccgattgcc cgtcttaaag tcaaagctgc cgtccggctg ctcatggtca
    42841 ttccagaaca cataaaatgc aatagtattc agccccatag ccttggcggt cctgatgcgg
    42901 tgcctccagt attcccgggg aatgcgctgg ggatgcatct ccgcgccgcg gatctggaag
    42961 ggtttcccgt tcagcatgaa ctcctggttc ccttcccctc cgaacccgaa cacaacgggg
    43021 ggtcctccgg aaagagcggg tttctgtaca ttctggacag cctcaggacc agcccatgcc
    43081 aatgctccca ctccgacggc catcagtgcg gcagaccaac tgaaaccata ttgcttcatc
    43141 atgttgaaca gttaattcat ttgaagacgg ttgccaataa caattttttt ctctcttctc
    43201 cgaatcttcg gaataccctt cccctccgcc agaaatctga cggggccgga cggaacggag
    43261 gccttccgtt ccgtggaatg tgatcatgtc atatatcttc atccctttgt atgaaaaccg
    43321 gaggacgcct gtctaaattg atcaatatga aaacaattgt tcttcttcgc catggagaaa
    43381 gcacgtggaa ccgtgaaaac agattcaccg gatggacgga cgtggatctg acggaattgg
    43441 gaatacagga agccaaccac gcgggcgacc ttctgaaaga caggggaatg gcctttgacc
    43501 atgcctacac ctcttatctc aagagggccg tgaaaacgct caactgcgtg ctggaccgtt
    43561 tggaccagga ctggctgccc gtctccaaaa gctggaggtt gaatgaaaag cactacggca
    43621 tgctccaggg cctgaataaa agtgaaacgg ctcaaaaata cggagacgaa caggtactga
    43681 tctggaggcg cagttatgac gtggcgcctc ctcccctggc ggaggacgat cccgccaacc
    43741 ccaaatggga tccccgctac aagggagtgc cggacagcga acttccgcga acggaatcac
    43801 tgaaagaaac tattgcccgc atgatgccct actgggaagg aacagttctc ccttctctac
    43861 ggacgctgga caatatcctg atcgtagccc acggcaacac attgcggggc atgatcaagt
    43921 atttgaaaaa cattcctgac gaacagctgc tttcccttaa tcttcctacc gccaccccct
    43981 atgtatttga atttgacgac tccctgaacc tggaaaaaga ctactttctt ggggatccgg
    44041 aagaaatccg caaacgcatg gcggccgtcg ccggccaagg aagcgcaaag aaaaaaacgc
    44101 ctgcggaata acctcactat cctttcctat gaccgtccat tcctgccgca ttgcgttctt
    44161 tgacgccaag ccctacgacc gggattcctt caattctgtc aatgaacgcg aattccatta
    44221 cgacatccgt tacttcaagg ggcgcctcac tccggacagc gtgcccctga cccgcggtac
    44281 ggacgtagcg tgcattttcg tcaatgacac ggcaaaccgg gaagtaatac ggaacctgaa
    44341 ggaaaatggc gtcaaattgc tggctctgcg ttgcgccgga ttcaataatg tggatctgaa
    44401 agctgcggaa gaagcggaac tgcccgtcgt gcgggtgccc caatattctc cctatgcggt
    44461 ggcggaacat gccgtagccc tgatgctttc cctgaaccgc aaaatacacc gcgcctactg
    44521 gcgcaccagg gacggcaatt tttcccttca cggcctgatg ggctttgaca tgaatggaaa
    44581 aacggccggc atcatcggaa cgggaaaaat cgccagaatc ctcatccgca ttctcaaagg
    44641 cttcgggatg aacatcctgg cctatgacct gcaccctgac cagcgctttg cggaagaagc
    44701 cggcattacg tacaccaccc tggacgatct gtacgcccgg tccgatatca tctccctgca
    44761 ctgtcccctt actccggaaa cggaacacct gattaatacg gattccattg gaaaaatgaa
    44821 ggatggagta atgatcatca acacgggacg cggcaagctg attaacacgg aaatgctgat
    44881 tgacggcctg aaatcaaaaa aagtgggagc tgccggactg gacgtgtacg aagaagaggg
    44941 agaatatttc tacgaagaca aatccgacag gattattgat gacgatactc tggctcgtct
    45001 cctttccttc aacaacgtca tcctgacttc ccaccagggc tttttcacga aagaagccct
    45061 gcacaatatt gcggaagtca ccctgcacaa catccgggac tttttggaaa gcaaacctct
    45121 gatcaaccga gtctcgcttc agtcacgctg aacgtgcccg gcgggcgctc tacggaaatc
    45181 cgggactttc tcatccgcct tctgccggac agaaggcgga attcggggaa aggcggcagc
    45241 ttttttatgc aagcgccgcc acttcccgcc gtattatata ctatgagcac tccagcccct
    45301 gcctccgtcc tggctcccaa accgcactac gccgctctgg acggcctccg cggggtggcc
    45361 gccgtcatgg tggtcctgtt ccacatgttt gaaggcagcg ccagggactt ccagttccat
    45421 acggaccaga tcatcaacca cggctacctg tccgtggact tcttcttcat gctgtccggc
    45481 ttcgtcatcg gatacgccta tgacgaccgc tgggaaaaaa tgaacctgtg gaacttctgc
    45541 aagcggcggc tggtgcgcct tcaacccatg gtggtcatgg ggatgctgct ggggggaatc
    45601 ctcttttact tccaggggtc tgaaatcttc ccgaaccttg cgcaaactcc cgtatggaaa
    45661 atgctgctgg tcatgaccgt gggattcacg ctgctgcccg ttcccgtttc cctggatgtg
    45721 cgcggatggt cggaaatgca cccgctgaac ggtccggcct ggtccctctt cttcgaatac
    45781 gtcgccaata tcctttatgc catgctcatc agaaaattta ccagcagatg gctggcggtc
    45841 ctggtctttc tggcgggctg cgccctcatt caccagtgct acacgcaggg caacaacatc
    45901 ggcggctgga ctctggatgc ggaacagtgc cgtgtggggc ttacacggct catgttccct
    45961 ttcttcggcg gtctgctcct gttccgcctg tgcaaacctg gacggatcag gcatggtttc
    46021 tggtggtgca gcctgcttct ggcgggaacc ctggcggcgc cccatgtagg aggcgcggaa
    46081 catttccgcc tgaacggcct gtatgacgcc ttctgcatca ttgtcatctt tcccgtgata
    46141 gtcttcctcg gagccagtga catccctgga gacatctctt ccagagccag ccggtttttg
    46201 ggaaacatct cctatcccct ttatatcacc cactatccca tcgtatatct ctacctggcc
    46261 tgggttcacg atgcggaagt ccctctttcc cgtgcgctcc ccgtagcggc aggcgtctta
    46321 ttcctctgca ttgccgtagc ctacgggtgc ctgaagcttt atgacgagcc cgtgcgcgca
    46381 tggctttcca aacgcgtgct gaagcggcat cctccggaac ccggggcgta aagcggaaca
    46441 aacatctgcc ttgccccggg cggggccagg ccggatactg tcggcggtct ttcatggaaa
    46501 aactgccgac atcctccaaa gctctgcgct acctgccctt tcttgtggca tcggcattct
    46561 ttatgcagat gctggatgcc accatcctga acacggccat tcccaccatt gcccgcagct
    46621 tcaacagcca tccgctgcag ctccactccc tggtaacggc ctacatgctc accgtctgcg
    46681 tcctgatacc ggcctccgga tggatatccg acaaactggg ttcacgccgt actttcctgc
    46741 tggccatctc cgttttttcc gtaggctccc tgctgtgcgc cctttccgcc tccgttacca
    46801 tgatgacggt atgccgcgtc atccagggaa taggcggcgc cttcctgatg cccgtcgggc
    46861 gccttgtcat cctgcgctcc tatccccgct ccatgttcgt caatgtcctg aacattgtaa
    46921 cgattccggc gctgctgggc ccccttctgg ggccggtgct gggcggtatc atcgtacagt
    46981 acgcttcctg gcactggata ttcctgatca acatcccggt aggcatggcg ggcctgtggg
    47041 ccacctggaa gctgatgccg gacctgaaag cggtccggga acagaaattc gactggccgg
    47101 gtttcttcct tttctcctcc tccgccgtcc tggctacgat gggcctctct tccgcaggag
    47161 gcataaccga taagccgcgc atggtcatcc tcacgtctgc cggaggcatt cttcaacttc
    47221 tgtactgggt gctggcgttc cgttcaaaag cccccatctt cagcccggcc ctgttccgca
    47281 tcagaaactt tgccatcgga atcgccggca acatcgtctg ccgcctgggc ggaagctgcc
    47341 tgccgtacct gatgccgctt ttcttccagg tggtgcttgg ttattccgct cttcagtccg
    47401 gcatgtccct gattccgctg gccctgagca atctgctggc aaaaacggtt gcgccgcgtc
    47461 tgctcgggaa atttggttac cggaacatca tggtcgtcaa taccttcacc ataggagcgc
    47521 tgctggcctc cttccacttt gtaggccccg gaacccatga acttattctc ctgggcatgc
    47581 tcgcgttgct gggcggggcc aactccattc agttcacctg catgaacacc ctgacgctga
    47641 ttgacctccc caatgcagac gccagcagcg gaaactccct gctttccacg atcatgcagc
    47701 tctccattgc tgtcagtatt gccgcggcct ccctgctgct ggacagcttc ggcggccatt
    47761 cggctacctc cggaaaggct gtggaaaccg cctttcatgc cactttcgcc accatcggcg
    47821 ccattgccgc ggccagttcc ttcatcttcg ccctggtgga caaagacaag ggaatcatcc
    47881 gcaaacggaa aaaagcagct tgaagaggaa aattcccgcc cctgttcagg aacaggaaga
    47941 ggaatctccc gtctcaagag aacagcactg cgcaaaccgg gcgcgggact cttccagggt
    48001 aatcggtttc ttatcctccc ccagggctac ataaaccagc tccgcctctg taattttggc
    48061 aatgttattg caaagcttcg tcagacgttc cgcatatacc tccaccttca cggtgacgga
    48121 ggtgcggccc accttgataa tatccgtata aaaggacact acatcgccca ccagcacagg
    48181 agcttcaaac gtcaggctgc tcaccgcacg cgtcacaata tagcggctca atgcatagcg
    48241 catggcggta ttggctcccg caaggtccac ctggctcatc acccagccgc cgaatacttc
    48301 cccgctctga ttggtatccg caggcattgg ttccacgcgc agggcgggaa cgcgcccttc
    48361 cggaagctgg gcatgacgat atgtattttc cataaataag aattattata aattaataag
    48421 gacatgctta tctatcacag ccctcttgta aagggtaaaa cgcggaagcg gaaaacgggc
    48481 ggcgccggca cgctctgcgg aaaaaagaaa acagcccgga ataaactgct ttcccatatt
    48541 gattaagttg ggcaggcgga tttccgtgtc ttatcccgga gcttttccac catggccggt
    48601 aaaggcggtt gtccattact catggaccgc aaccatgaat ccaaaacaaa aaaagtggac
    48661 ggggaggcac atcccgcttc cgactttgcc tacgtacccg atccggaaaa gccttccacc
    48721 tggaaactgc cgattttcaa catgcgccac gtaggagggg ccctggccgc catgaccagc
    48781 gactatcgcg gcaacccc
//
LOCUS       DAWEMX010000006        38656 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_426_length_38656_cov_14.875780, whole genome shotgun sequence.
ACCESSION   DAWEMX010000006 DAWEMX010000000
VERSION     DAWEMX010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 38656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 38656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38656
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_426_length_38656_cov_14.875780"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10011..10020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11734..11743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8035 a        11350 c        11248 g         8003 t
ORIGIN      
        1 tcgtcactct gcgcggcatg tggagtcacg cggatatcac gatgattatg cgcggcctga
       61 gccagaaaaa aggcacagac atcatgcagc atccttccgt gattgtgcgc cccggtgaaa
      121 aagccacctt tttcagcggc agggaactga tttaccctac ggaatatgat ccgcctgaag
      181 ttcccaacag cacgggcaat aataatgact ggggcgacaa tgacaataac ggcggcggca
      241 ttccggtcat gcccatgact cccgcccatc ccactgcctt tgaaaccagg cagcttggaa
      301 cgatcttcaa tgtggaaatg accgtagtgc cggaaatcgt ggattttgac ggtttcatca
      361 attacggcac gtcgcttttc gttcccatgg tctcccagga aaaggacgca aaggaagatg
      421 tcgtcatggt gaaaacttca gacaacttca tccttcagcc cgtgttttcc acgcggcgcc
      481 tgaccgctcc ggtgcgcata gccaccggca atacgctcgt catcggcgcc ttgaaaaaat
      541 ccacttccat tacgtatgag gataaaattc ccgttctggg ggatattccc tgggtggggc
      601 gccttttccg ttccaagggg tccaaggaac agcgcaaggc catcatcatc atggtaaagg
      661 cggaagtggt ggatccgggc ggcaagaagc tttatacgcc ggatacttcc attccggacg
      721 aggaggtccc ggcaggggac gcttctctcc ccgccctcag caatgtccag taacctttaa
      781 ccggatcaag accattgaaa aacagcggtt ccgattttga gtcttctcct tcccaggggg
      841 ctgataatat catgcgtcag ctgcatgcgt cggattccca cggagtagaa cggagaaggg
      901 aagtagtagt gcgcccggac ggcagcaagg tgatccgggt ggaaaagcgc cggcggactt
      961 acagtgacga ccagcgggag ccaaacaggg ccatgttgaa aaacaagcgt tttttaatag
     1021 gcctttgcat tctggttctg ctggctgccg gtgcgctggc ggggacgtat atgtaccgcc
     1081 ttacttcttt caatacggag gaatttgccg gccgtctgca agctgatctt tccgccgcct
     1141 ggggcggaga tgtgaaaatt tccggaatgg cggtggatgg ccttaccatg aaggcttcac
     1201 gcatcagggt atcctttccg gaagattcct gcctgtcctt tgtggcgatg gaaggcatct
     1261 ccggggaaat ttccgccgtg cccttattca tggggaaaat caagggggaa agccttaatg
     1321 tgtccagggc ggtagtcggt ttgcggcccg gattcgttaa atttgccgta ccgcagacga
     1381 aacgggaatt gccgttcgtt ttccggcgat acacagcgcc gcgtctggaa gtggggtatg
     1441 cccatgggac ggaagaattt actctggaga ggaatggcgc tcccttttat atgacggcgg
     1501 aagcctacat ccgcacgtcc gggaacggac ccgaaccgga atacgtgctg gatttgtcac
     1561 agcaaaaatt gaaaatcaag ggctggccgc tgatgggggt tgattccggt tccgtcattc
     1621 taaacgggag cggcgtcaag cagctcactt tttccggttt tctggacaaa gggcgtctgc
     1681 tcaataagcc tacggatctc agccccttca tgattacggg gaatttcctt ctgggtacgg
     1741 attttcgtca ccaggcgtgg actttgacag gaaaaaattt cgatctcgct tccctgctgg
     1801 gagatgattt ctcttccctt ctcaaggtgc agacgggaga acaggaaggg gatgaaaagc
     1861 atgaattgaa gctggatttt gctttgcctg taacaactga acagaagagg ccctccctaa
     1921 aaggatacag cggttccgtt atcaaggcga ccatgatcag tctgcctgtt ttgcgcctgc
     1981 tggcggccat ggctgcccgg aatacggaaa taaggctgcc gtactcttct cccgtcttta
     2041 cgtccggttc tttcctgctg gaaagcgatg gaacgggccg ttccgcctcc ctgaaggaaa
     2101 tttccctgtt tgagcagggc tttctgggag tgaccggggg ggtgtacagt aacggtactt
     2161 ccttgacggg gaagctggtt tttaaaattc cttcctacat ggtggacagc cggaaactgc
     2221 cttccggcgt gacgcaggaa gggcatgaaa ttattcttcc tgtgaaaatt tccggtactc
     2281 cggagtctcc acaggacaac tctgccggaa tgttccagga agaggcgccg cgcacgggcc
     2341 gcccggaaat gcataccgct ccttcccgtg gaatagctct tccagctccg tcaaattcct
     2401 ctccttcccg tattacggta gacggattca tgtgatattg ccaggactcc atgctgctgc
     2461 tcaatcctat tgttcggaaa acatctgaaa agcgttcccg ctggtggacc aggctggccg
     2521 gatttggcct gtggtccgta ctccagccca tttgcatgag cctgaatatc cgttccatca
     2581 aggaggagca tgatgatgtt tatacggcgc cttttctggt agccctttgg cacaaccgca
     2641 cgatggtgcc gggttatgcg tggtccctgg cccagaagcc gctcaagatg agcgtgctta
     2701 ccagcgccag caaggacggc gccctggtgg aggccgtatg caggcatttt ggcctgggtg
     2761 ccgtccgggg atcttccgga cggcgggggg ctctggccta catggaaatg atgcgaaaga
     2821 tccaggaggg ggacgtctgc ttctgtttta ctccggatgg tccgcgaggc cctatttacg
     2881 aagtgcacca cggtattgtc aaactggcct cccagtcagg gctgcccatc gttcccgtat
     2941 gtatcgaata tgaaagctgc tggcgtttga acaagacctg ggaccgctat gccattccca
     3001 aaccgtgttc caacgtaaat attctatgga aaaaacgcct gttcattcct ccggacgcaa
     3061 cggatgaaca ggtggcggat tatgcccgtc ttctggccgg gctgcttggc gaggggcttc
     3121 cggacttccc gcctcttaac caatcattat tatgcaaatc atcgatggaa aacaagtagc
     3181 ttcccaggtg ctggaagagg ttgaaaggga tattgcccgg ctcagggaac agggaataac
     3241 gcccggtttg gccgtggttc tggtagggga agatcccgct tcccaggtat atgtaaattc
     3301 caaggtgaaa aagtgtgcgg agctggggat gaattcccgt aaaatagttc tcccttccga
     3361 cgcctcccag gaagaactgc tccggattgt ccgtgaattg aacgtggatc ctgccgtcca
     3421 cggcatactg gtgcaaagcc cgcctccccc ccatatcgac gaggcggccg tggtcctgga
     3481 aatagacccc gccaaggatg tggacggctt ccatccggag aatgtcgcca agctggtgct
     3541 ggaagatgag accggttttg tgccctgtat gaggctgtta aaggccgccg gcattgaaac
     3601 ggcgggagcg aatgccgtgg tcattggccg cagcatgatt gtgggcaaac cgatggcgca
     3661 tcttttgatg tccaaacagg caaacgctac cgtaaccgtg gctcattccc gtacgaaaaa
     3721 cctgccggaa ctttgccgtt ccgctgatat catcgtggca gccataggcc ggcccgcttt
     3781 tgtgacggct gattttgtga aagacggcgc cgtagtggtg gatgtgggca tcaaccgcgt
     3841 ggaagatgcc tccgccaagc gcggctatcg gattgtgggg gacgtagcct atgacgaggt
     3901 ggctcccaag tgccgggcca ttactcccgt gcccggcggc gtggggccga tgactatcgc
     3961 catgttgatg gctaatacgg tcaaagcgtg ccgccagcag acgggagcct gacgaaaatg
     4021 ccgtgacgtc cgtttttctt gtcaaatgaa ggaaagtagg gcagtattca gttaccatta
     4081 tgtcaagtaa attactcgaa ggcaaagttg gtgtcgtagt cggcgtagcg aacaagcgca
     4141 gcatcgcgtt cgcaattgcc aaggcgtggc atgaagcggg cgcaaagctc atcttcaatt
     4201 atcagggaga acgtctcaag gacggagttc tcaagctgat ccatgagaac ttcggagagg
     4261 atgttcccgt ttatgatctg gatgtcagca gtgatgaatc tgttaacgct ttctttgaaa
     4321 gggtccgcca gcatacggat aaagtagatt gtctgctgca ttccgtggct tttgccccga
     4381 aggaggctct tggcggcagt ttcctggaaa cgggccgcga ggcgttcaag atttccctgg
     4441 atatctccgc ttattccctg gtagctcttt cccgcgccgt ggaacccatg atgtccgaca
     4501 acggcagcat tttggcgatg tcctatctgg gatccgaaaa agtggttcct aactacaatc
     4561 tgatgggagt ttccaaagct gccctggaag cctgcacccg ttatctggct tatgatttgg
     4621 gccgttcccg cggtatccgt gtcaattgca tcagtgcagg tcccatgcag acgcttgctg
     4681 ctcgcggtgt ttccggtttt aacaccatgt tcaaggtgta tgaggagcat gcccctctgg
     4741 gccgttcctg cacgggtgag gaattggggg ccaccggcgt tttcctggcc agtgacggcg
     4801 cagccgcaat caccgggcag gtgatttacg tggacggcgg ttatcagatc atgggaatgt
     4861 aacgcgttcc ttgcagaagc tttcttcctg ttcacaagcc gtaccggttt ttccggtacg
     4921 gcttgtttac ggtttcatgg agaaggtaag gggggctggg ggcttcctgg cttctgtcag
     4981 aaaggcctgt ttccccgctg cgtttcaggg gacgaacact ttcagcgagc cggggcgggt
     5041 gatgcggacc ggagcgtttc ctgcataatc gccgtccagt tcgcaggagg cctgtccggc
     5101 ggaggtgacg gttccccccg tcatccggat gtattcaaaa ctgccgctcc tccgggaatg
     5161 aaagccccct tggactgcgg ccatcaggaa ttccctgatg atggaagtaa tggaatgcct
     5221 gaatactact gcgtccagaa ggccgtcact gttgtccgct tcagcaaaga agttaagcgg
     5281 cccgccatac ttgcgtccat tgccgaagat gatggaacgg ccttccaata cccggccttc
     5341 atctgtggtc agggtgaggt gcggctgttt ttccgtacag acgcgggcgc cggcaattac
     5401 ataggcaagg gctttccatt tttttttcat ttcccatgtg gtcagttcta cggctcgggc
     5461 atctgcgcca atgcctgcca tttggagaaa aacttgcccg ttgaatgcga acaggtccac
     5521 atttttggaa cgccccgtgt tcagaatgtg cagggcgtgg tcgaaatccg ggcggattcc
     5581 tatttcccgt gaaaaaacat tcatggtacc cgcagggaag ggggccagca ccgtttccgt
     5641 attgcagaga gcccttgccg cgattcccag cgtgccgtcc ccgcctgcca cagcaacggc
     5701 aggcgctccg gaattagcct gagaaaccaa atggttcaac atgtcttccc tgctttccgg
     5761 ttcaatgacg tcaaaaaaat cccgatgccg atccagccag cggcggaatc gtccggctct
     5821 ggcgctgcgc gccgtgttat tgaaaaacag gggaattttc atggagcgga cacgaagaag
     5881 ttcaaaatct gaaccggggc ttccgtgtgt gccacaggtt catggcgcac ggttccgtca
     5941 ggcagcttga actccagcat gccgcgggaa atattgtccg gcaggagtac ctcaagaaac
     6001 ttcctcatgt cgtcggacgg tccgggtgta atgccttccg ccagaacttt cccttcttcc
     6061 gatttgagga cgacgtgcaa aggaagcctt ctggcaccgt tttttctctc ttccctgatg
     6121 tccagcatca gccggctgcc gggcacatat tccggttggg ccgccaccca ttcattcgcc
     6181 agtttcatgt agcggtccgt gatattgacg gcggggacgg tacggctttc cggagggaaa
     6241 gccagcgcgt gccgtttgtc gtcgtagcgg gcttcccata tcataggaaa aaaggctcct
     6301 gaaggttctt ttttccagga ggtggcataa atggcgttct ccggtttgcg gggatccagc
     6361 atgctgatgg cggacatcac ccacggggtg ttgaagtcct tttcattgta ttccagcatt
     6421 ttccattctc cgtcacacca ggcttccacc cagttgtggt tgccgcgcac gtggttccag
     6481 gtaagaacgc ccgccatgcg ggccggaatg cccacggagc gaagagcgca gattagcagg
     6541 atgctttggc ccgtacagga gaccttgccg gattgcaggg attccttgac tccctggtgg
     6601 ggaactcttc tctccgtgga atagcgtaca ttcagggttt tctggatgag ggaggcgatg
     6661 gcgagggcgg cttcccgccc cgtcttgcat ggggaaacaa gcggtgcgaa gaggtgatgg
     6721 aactgctccc tccattcgtc tcgcggttcg ttgaccacgg cataagggag aacattattt
     6781 tcataaagtt cccacggaac ctgggcagcc caggggaatt ggctccgtgc agccagggcg
     6841 aggcggactt cccggaccag cctttcttgc gtaaccgttc ccctgtcctg cgcgggcatg
     6901 tgggcacgca gaaactccac cccgtcctga atggcgtcat gggcggcgca aatagcggcg
     6961 gtcaggcccg ggagaagggc gaaaggcagc agattcatga acgttgaaat tcatacctga
     7021 attccgggag ggagacaagc ttcctcttgc gcaagaaggg tatggaaatg ggcaaagaga
     7081 tgcgtgtctt ccggcggagt gggggcaaag ggtgcgtcgt tatccagaat atcctcgcaa
     7141 atgcgcatga aatcgcgggc ggttttcgcc cttttcatgt aatggtcaaa agtgtcggga
     7201 agtccccgag cggtataaac cagatatttt ttcatgcggt ggatgtgttt ttcctccaca
     7261 taatgttcct gcatttcccg cgtgcgttca taaaggcggc gaatatagtg cagcacgttc
     7321 cggaaagtaa ggtctgctgc aggatggccc tgaaagcggg aatgaagctg tgaaaatatc
     7381 cagggattgc gcagggctgc ccgtccaatc atcagcccag ctggccgggc cagccgcacc
     7441 catgcctccg ctgtggctgc atccacaata tttccgttgg cgactactgg gcatttcagc
     7501 attcctgcgg cccatttcac ccattccggg tgtaccgggg agcggtagcc ctcacggacg
     7561 gtgcgggcgt ggatgcacac cctgtccggg gaatgggagg caattaccgg aagaatccgt
     7621 tcaaactcgt ccggcgtttc gtatcccagg cggcatttga cggtgaaggc tccggggggc
     7681 aatacgtccc ggagcgctcc cagggccgcg tccatggcct tcagggaccg cagcatgcct
     7741 ccgcccgcat ttctccggca tacgatggga gcggggcagc ccatgttcag atccacgcct
     7801 gcacaggcgt gttccatcag gacctgcgca tcccttgcca ggtattccgg ttcatggccc
     7861 acgagctgtc cgtaaatggg gcggcctgta ggattttcat caatggagcg cagaatgtat
     7921 ggcgacatct ttttatgatg ggccaccgtc cggaaatact ccgtgatgaa ataatccggc
     7981 agggaattca agtccttcaa tgtgttgagg aaagccagat ccgtcacatc tttcatgggg
     8041 gccagggcca ggagcatggg tctgtgcata ccgtgaaaag ctccggttgg caagctgtga
     8101 ttgtcttgac gaaagaagga aagtgtggaa gctgggagca tgaatcagga ctcaacccgc
     8161 aaagccagag tgaatgtgag acgggccgaa gtaatggagc aggtggaaaa ggaaatccag
     8221 cagcattacc agagtgaatt gatttcccac atccgttctg cgggcaatgt gtacaatctg
     8281 ggccatacgg aattttttct cgcccgggag ttcgggttct gcaacggcgt gcgccgtgcc
     8341 attgatatag catatgcggc acgcagagtg tttcctgacc ggcggatttt tctgattggg
     8401 gacattattc acaatccgga agtgaaccgg cagctggagg aaatgggcat tcgaaaactt
     8461 ccctggaagc agttggattc ctcctatgac cgggttgctc cggacgatgt ggtgattatt
     8521 cccgcgttcg gtgttcccac tcctttcatg gatgcgctgg aggagaaggg cgtgcagatt
     8581 gtggatacga catgcgggga cgtgatgaag gtctggaaaa gagtgaagaa ttacgccgcc
     8641 atggggatta cctccattat ccacggtaag gctacccatg aggaaaccag cgctacggct
     8701 tcccgggctc ttggggaacg gggaaggggg aaatacctgg tggtttacga tttggaggat
     8761 gcccgtatcc tgtgcgacta catcatgggc cgcggagacc gcgaggcatt cctgaaacgg
     8821 tttgaaggat gctgttcccc gggattcgat cccgaccggg atctggagga ggttggcatc
     8881 gccaaccaga ccaccatgtt gaaaacggag acgcagacgc tccagaagat ggtgaaggat
     8941 gccattgttc agagggacgg ggacgatgat aatttttatg tgtttgacac catttgcggt
     9001 gctacccagg atcgccagga tgccctgtat gaactgctta aaaatcctct ggacgtcatg
     9061 tttgtggtgg gtggctacaa cagttccaac acaacgcatc tggtggatat tgccagggag
     9121 catgtgccca cgtacttcat tgagtccgca gaatgcatca agtccatcca gtatgtggat
     9181 gcttttgata cgaagacgcg ggaagtgcgc cgcatgacta cggaaccggt agtgcagaat
     9241 ctgggcaaat ccctgaaggt gggaattacg gcgggcgcct catgtccggc caacctgatt
     9301 gaggccacca tcctccgcat tgcggatctg cgcaagtagc aaaacgcctc cggtttggcg
     9361 aatgagaaaa aagaagagtt ctctttcaac ggcggcacgg ccgttttttc cgtaacacgg
     9421 cgcagtagcc tgggctgcgg aagaaagcat agggaaaact gcgcgggagc gtttcctaaa
     9481 tttccaaatc ttccggtgta acggaacgtt gttcccggga tgcaagcagt acggcttgtt
     9541 ccacgacata ttccgtattg gagtcaaaac ggtaggcgtt gcagttggga cacactgccg
     9601 tagtcaggga tacgatggaa ccgcaggctt cgcaaacctt gaagtccccg ggacggtcaa
     9661 taatcagttt ggctttatcc aggcgggatt gctgcatggg agtagaaaat aaaacaagcc
     9721 gctcagcaga acagagcggc ttgccataaa gaatcgtcag aaaaccgaag tgaaaactca
     9781 ggcgattttt gcgatggcct tggcggcctt gctcttgtag ttggctgcac gattggcggg
     9841 aatggtgcca accttggcgg ctttgtcaac ggctgaggag aaagtgttgt aggcagcagc
     9901 agcggcttcc ttgtcggacg tttccaccgc agcggcaact ttcttgcgga gggtcttcaa
     9961 cttggaggtc acggcgcggt tgcgggcggt gcgggttgct gtctggcgga nnnnnnnnnn
    10021 ttcggtttcc gtttcgaggt ggcaaaaaca ttagaataac aggacaaaat gtcaagctct
    10081 tgatgtattt tttaaggcat tcctttcctg cacgggctga ctgtgggctt taccggaaaa
    10141 ggagtttgtg gcactatatt ctcccgttat gaagaaacgc gtggatttgc ctgaaaaaaa
    10201 atatgaagga tatatttttg atttggacgg caccctggtg gacagtatgc cgctccatta
    10261 cagggcatgg cgtgaagccc tggctcgagc gggggctccg gaccatgtgt tccgggcgga
    10321 tgaattttat tcctgcggcg gaaaatccgc caatgatgtg gtgcgttttt taaatgaacg
    10381 gtacggcatg cacatggatg cggcgtccac agctgcggac aagcgccgca tttatttgga
    10441 aatgctggaa aaggaaggca tgcagcctat ccgggaagtg gtggaattcg tgcattccct
    10501 gggagacgcc cccaaggcga tagctaccgg gagcgccatg ccgggagcgt cccggactct
    10561 ggctgctgcc ggactttcag gcctttttga tgtgattttg acgccggatg aggtggagca
    10621 tggcaaacct gctccggaca tgtttttgaa ggcggcggaa ctcctggggg catccccgga
    10681 ccgttgcgtg gtttttgagg atgccgaacc cggcatgaaa gctgccgcgg ccgcaggaat
    10741 ggactgcgta caggtccggc ggccatccct gtcctgattc ctctttccgg atttggtacg
    10801 gaaggggatt ccgggatttt cctcctttct ccatgccctt cttcctttta aggaaaaggc
    10861 ttcacattgc gcccggaatg cgttagaacc ttttttccta ttgaacaaat aaaatattat
    10921 ttgcacatga gcacaaacga caaaacatca tccgccccga cgaaaacccg catgacagga
    10981 gctgaagtgc tcgtggaatg cctcgtgcgt gaaggtgtag acgtcgtctt tgcctatcct
    11041 ggcggcgcca gcatgccgat tcatcaggcc ctgagccatc agcccaagat acggaccatc
    11101 ctgccccgcc atgaacaggg cggggcattc ggtgcggaag gctatggccg cgttacgggc
    11161 aaggtgggcg tttgcatttc cacttccggc cccggcgcca ccaacatgat tacctccatt
    11221 gcggatgctt atctggactc cacgcctctg gtggcgatta cggctcaggt catgcgctcc
    11281 ttgattggac gcggcgcctt ccaggaaaca gacgtattcg gcatgaccgc ccctatcgtg
    11341 aagcacagct atctggtgac ggaaccggaa gaactgcccc gtatcatcaa ggaagccttc
    11401 tacattgcct ctacgggccg tccgggccct gtgctgattg acatgcccaa ggacgtgcag
    11461 gaggccgtgt ttacaccgga ctttgacatg gagatggata ttcccggcta caaccccgtg
    11521 ctgcccgtcc ctgcggacaa gctggaagaa ctcatcccgt tgattgaaaa cgccaggcgg
    11581 cctgtcattt acgccggtgg cggcatcatt tctgcggaag cttccgggga tcttctggaa
    11641 tttgcggaac gttcccagat tcctgtggcg accaccctga tgggcattgg cgccatgccg
    11701 gaaacccacc ctctttccct gcgttggctg ggcnnnnnnn nnngcacgga gccgtgtttg
    11761 cgaataacgc cgtgaatgaa gcggatctgg ttattgccct gggcgcccgg tttgacgacc
    11821 gcgtgacagg cgccgtcaat atgttctgtc cggaatccac gatcgttcac attgatatcg
    11881 acgccagcga gatcaataaa aacaagaagg tggcgtatcc catccgcgca aacgtgaagg
    11941 acgccctcca ggtgctcaac tccgctctgg ccgcaaaagg ctgggagcgc aaatcctccg
    12001 gctacacccg cactcctgaa tggttcggcg tgattgacag ttggaaaaaa cagtacccct
    12061 tctcctatga ggaccgcaag gggtatatcg ccccgcaggc tgtcattgag gagctctacc
    12121 gccagacggc ggacaaggac cccatcatct gcacgggggt gggccagcat cagatgtttg
    12181 ccgctcaatt cttcaaattt gacaagcccc gccgtctggc cacttccggc ggtttgggaa
    12241 ccatgggcta tggacttccc gcctccatgg gagcgtgcct ggcttatccg gacagactgg
    12301 tcgtgaatat tgacggggac ggttccatgc tcatgaatat tcaggagctg gcaaccattc
    12361 atgtggagcg tctgcccgtc aaatgcatta ttctgaacaa ccagcacctg ggcatggtgg
    12421 tccagtggga agatttgaag tatgattcca accgggccca aaccttcctg gcggacccgc
    12481 acgacaatta tgatcctacc cataaaacgg aagatgtcat ctacccgaac tatccgctta
    12541 tttgtgccgg attcggcgtc aagtgcgagc gtgtgcttcg catagaggag cttccggcgg
    12601 ctatcacccg gatgattgaa tcccctgagg cctatgtcct ggacgtcatg gttccccatg
    12661 atgtgcatgt gctgccgatg attctggggg gcatgagcta caaggatgtg attctggaac
    12721 gtattgccgg ggatggttcc gccaaaaagg cgtctgaact gggcaaggaa atcccgactg
    12781 ccctgtaaga gcgaggcaag cttcttctat tgagccttaa acaggagccg tccgtaaggg
    12841 cggctcctgt tgcatggcgg cgcaagagga agacaggtca gttgactgcc gttagggtgg
    12901 cagcaaggca tgtatataga tccggatatt cctgttttcc ggtaggtgaa ttcctggttt
    12961 tgtgaaggaa tgaggaagat gtttctggac gcagtaaaaa agaacggtta gcatcaatga
    13021 gccaaattga gtgcaagctg gctcggacgt gaagtaactg aggttttcta agatgatgcc
    13081 acatcggatg ctgcgcttgc gccaacctca agctcacaga aaatttccgg gaatccggcg
    13141 catgctgtaa gcgattgaga aatgtagctt gacaaaaaaa ttccctttga tcaggcctga
    13201 ccgcatctgg ttcttgcatc aggttggcgc aagacctttg ttgcatgttt gatactcaaa
    13261 ccgtattcgt caggccgtcg caccctcacg ggtgcgtgaa ttgaaacttg ttatcaactc
    13321 ttctccggca acgacaatgg ggtcgcaccc tcacgggtgc gtgaattgaa acaccatgcc
    13381 ccggtcggcg tcggaattgt aagcatgtcg caccctcacg ggtgcgtgaa ttgaaacttt
    13441 gtgcctgatg cagattcctc ctcccccccc cgtcgcaccc tcacgggtgc gtgaattgaa
    13501 acaatgtccg caggtctgta tttaacctca cgggtcgtcg caccctcacg ggtgcgtgaa
    13561 ttgaaactcc agcatgaact ttacgccatg tgcagagggg agtcgcaccc tcacgggtgc
    13621 gtgaattgaa acattatgct cctcaaggga agccttcttt acgcctgtcg caccctcacg
    13681 ggtgcgtgaa ttgaaaccgt gggaactggc gttggacgtg tactggaaaa aggtcgcacc
    13741 ctcacgggtg cgtgaattga aaccccggat tccctggcag tccttgattt cgaagggggt
    13801 cgcaccctca cgggtgcgtg aattgaaacc tcttcttcct gcatttccgg ttccggctct
    13861 gttgtcgcac cctcacgggt gcgtgaattg aaacgtcaag gtttcgctcc tgcatgttgt
    13921 catcgacagt cgcaccctca cgggtgcgtg aattgaaacc agaaggacgc ttgtcaggca
    13981 ggcgaggttt aatgtcgcac cctcacgggt gcgtgaattg aaacttcttc ccgccggatt
    14041 aacagcccgt tcaggcccgt cgcaccctca cgggtgcgtg aattgaaaca taccgacgtg
    14101 ctccattttc aaaaacgccc gtagtcgcac cctcacgggt gcgtgaattg aaacgggttc
    14161 cgggtaagaa taatccccag tgaggaatga caggatagtt gatataagtt gaatcagggt
    14221 tgaaatccgt tggaacccta gaagacacgg gagttaagga ggatcagacc agtcatgagc
    14281 tatcttataa aagtcggagc tgaaaagagg gtaaatgaca agtatctgat ataagtacac
    14341 aggctatgca gggatgctgc acaagagctg caaagagtag gtgatagagc aacgacaagg
    14401 taaaaaatca tgataggatg ccccgcatgt gtacacctac atattgcatc cccttgtacg
    14461 gctccgttac cgttatatcc ccagcagacc ggaagaaaaa gaacccgcaa atttatcacc
    14521 tccatgatac cgctgtcgtg ttgatgtgga caactcgtga gggaagtaaa acgacgtgct
    14581 gggtgaggga tatgctcgac aacgtgtgga ttgtgatcag ggatgagaga gaagatgatt
    14641 gaaaagaaaa aggccgccag gaacaacctg acggcctgta ttgtatctgc ccattgggct
    14701 aatcatcgca acataaagcg cggacaaagg gcgtcatttc ctcgtcctga tggtcttcta
    14761 tcagcttgtc cgtcctttcg tccataatgc acaggacgct tgaataaagg ccgtcagcaa
    14821 atagtgattg cttggtggca acaacaagcg catgtactgc atagccggac tgctggccat
    14881 tgccatctac aacataacag ttgatgttgt ctcctggctt taaggggggc atgatgattt
    14941 ttctgttgtt tcaatccgcg catccgcagg gatgcgaaaa gttacagggg gaattcgtct
    15001 tcaaatgaca ccgtgtcgct ccatcccctg gccgttttcg gttcgggggc taggggggca
    15061 gctgctctcc ggccagcgtc ctggacggcc ctgggcactc ggacggctcc cacataattc
    15121 gccagccctc ccggagggag cgttcccggc agggcgtgcg gttcgtaggt ctcctggcca
    15181 gtttcggagg tcatcttgcg agactccgct cgtccccggt tgatgacggt gaacatcagg
    15241 tgctggacgt gttccacggc ggcccatttg cgcaatgcct ggtacacatc ccggcccgcg
    15301 caaggcaggt gcagctggtc gcgcacaatc tctgccagat agtccgggct ggtctcatag
    15361 cgctgcatgg catcgcgtag attgtggatg gccttgtcca tctccgacca ggtacgatcc
    15421 ttgataggtg cctcgcccag gtaggctgcc agtctattat agacaggcac gtagtgctcc
    15481 tgggtggcct tggtcagaga atcggtgact cctgtggcag cccagacctc gtcatgccgc
    15541 caatccgtca gggacgggag ggggcagccc tgggactgga tgcgcttgta agccttgccg
    15601 gccataatgg acagtacggc aatctgtcgc ttggtaagtg gcttattgtt catgatcggg
    15661 attttgtaga gccttgatca gttgttgccg ctgcttgggc gtcagataga caagagtggc
    15721 tgttgctcct cctctatcag tgcccgtcca gatagtcagc agggtctgga atccatagtc
    15781 tttaacctca atcttagtca tccctggtgc tcctctctac gtaaaaagtc tccacctgct
    15841 tgatctccaa ccccagcttg gcaagcttgt gaggcttgac gtgctggcgg attgcctcct
    15901 tgtccggggt caccttggtg accaggtagg ccctgcggcg ggtgctcttg agcagggcga
    15961 caatcttgtc ccacgtccag cccttggcgg gcttgaggga gggctgcccc aggcggtagc
    16021 cgtaggtagt cagggcggtg gtgccggatt tacggccctt ggcaaacagc tcgtccctgc
    16081 ggggggaggc ccattgctcg gccagtttgg tgagccgggc aatctccctg gccagttcgc
    16141 tgattttggg atcatgctcg gtgagcactt gctgcatggc ggtctccttg gcggcctgca
    16201 aggtgtccag ttcaacaccc ttgcgggcga tgtcgtccag ggtctgacaa aattcagcct
    16261 tgtccttaat aacctgctgg tcggttgctt tagtggttgt gcgtatcttt cccattgcag
    16321 tctgtagttg gttgttggtt acattggcac tgccccagca ggacgtaagt gagtacccca
    16381 ggagcatcgt ccggagtaag atcggcaaga gccacggtgt attgtttgcc gtgtcccttg
    16441 aggcctctcc ggaccaccgc cttatcacct gacaatatat cctcaataac cccatcatat
    16501 ccggctgatc tgagtgatat tctggttcgg ttggcagacc tgctgctggt catacaggtc
    16561 tggttgacgt agaccacatc tccaacctta aaagttgacg gatcgacggg tctgagcttg
    16621 ccgcagtgcg ggcatgtata tttctcattg tccattgtta ttgtgtattt ctcttgttga
    16681 gttgttttct ctttttggcc agcatgttaa cgacatgctg ccaggtaagg tctggcgtgg
    16741 cgctgagcca ctccagaaat cgctctccat ctacagggac gtagtactgt ccgagctgct
    16801 ccagccggat ggcgatcatg cccggccagc gggggtcgca gacttcccag tggtcacagg
    16861 ctccggggaa ccgggagagt tgcaggccga tgagataggc cagtgtattg ggatcgtagg
    16921 tcatggctgg tcaatgtaga tgtagacggg agggatggga ttaacaggct cggctctgtt
    16981 gatcagcttg tgtaccaggt catcagagga aagatgattg ccgtgattgg cgcgattcag
    17041 ctctgagcgg ataccctcgg tcatgattct tcggcactcc gggcagaccg cataggtctg
    17101 cccatggcgc tggatgatct ccaggcgcgc gtgccggtgg taatagcagt agatgtcctc
    17161 attcatgacg gcggccctct ttggaagcat caaaaaagcg aggcaggaaa cgcttactca
    17221 gttctcccgt gttgcgatta aggtgcatgt tgatgcctgt gacatcgtgc atcaggttaa
    17281 aagcatcagc ctgctccatg tcagcaagcc gcagtgggca cccgttgcaa tgacatgctt
    17341 ccaggtccat ctggatatcc atctcatcga cgtcggtatt gccgtattta cggtaaaggg
    17401 cgacagcccg cttggcgatg cgctcgatga tctcacgctc cgtaggtgtg gcggtaaaca
    17461 tatctttcat tgtttggcct cccttgggag ccggtttgac attgggggct tgccgccccc
    17521 tgtcctgttg attgttagtg ttggtgctgt acatgatggt ggtgttattg gttggaagtt
    17581 gagtcaggct ggtcggcctc ggtggggccg acggtgatgc tggccaggcg gatattgccc
    17641 agcagggcgt gcagatggga tatggccaga ttgagggagt catagtccgt cttggtcagg
    17701 agggccacct tgcaatggct gacccgatca acatggatat gctgggtgat gtaaagcagc
    17761 tgacggtgcc gttcccactg cgcctcgctc aataccggga taagttccag agaaaggctg
    17821 tgatgcgtgg tgatgagggc gtcgaggtca gccaggcgga ggaccacata tcgggttcct
    17881 cccaggtcaa cgatggtgta tttaatggtc ttcttgcgca tgattttttt gagggggggg
    17941 ttatacgtat tccggcatct tgccggcttc catttcttca agttttcgga cggcggccaa
    18001 gtagtagtcc catgtaaggt ccaccccggc attgttggcg gcctccaccc ctttgcgcag
    18061 gcgcttggtg gtcttgccga atccgtacaa attggcctgc tgcttgaggg tggccagcat
    18121 ggcccggttg ggggacggat agccgtaata agtccacagg gtttccaggt cgcgggtctc
    18181 gatatggtcc gggaggcggt agatattggc cgccccgcgc ttggaggtct gctccaggat
    18241 gcctttccac ttgtcatttt tttcgacgta ctcggccagg acgggcgtgc ccaccaggag
    18301 catgccgcac tgggtctcgt cgtagatctc ccgcagctgt tcaattcctt tgcgtccggt
    18361 cttgtcgctg tctagcgcgt ggtggatctc gtcgacaatg agcaggtgct ccggagtcag
    18421 gcggttgaca atcttggcaa tctggaactc ggtgttgcct ttgaccgaca ggcctaattg
    18481 ggcggcaatg cgatagagca gccgtcccgg actggtgacc accgggcagc gcaccatgat
    18541 aaccgtaccg ggatggcggc gggcgtactc tttgagcgcc caggtcttgc cccactgcgt
    18601 cttgcccacc agcatggagg cgtattggtt gacgtgggtc agctcggcaa tttgcatcac
    18661 gtagcgcgcc agagccgtct cgataaacgg cccgtcagac gctgattgct ggcgcagggc
    18721 cagcaggccg cacaggtcat caagcttgga aagatgaggg ccggtcgggg cctggtaagc
    18781 gccaatcagc aggcggtgca tgaccgtcgt gctcaccggc agtttgtcgg ccagcgtgcg
    18841 cagcgtccag tcatggtcca ctgcgtagtt gatcaggtcg gtgagcatct tcttgtgcgc
    18901 cggtttgtac ggcgtatcct gaatgcggtc cagatacctg ctcaggttgg cattgttgtt
    18961 gatttttgta atatcgtcca tattgttatt attgtgtctg ttatgttata aaaatcttac
    19021 atcgggcagg tcgtggggca gctcgtcgtc attgctgccg ctggcgggga tgaggctgat
    19081 gtccggcatg gactcggcag ccgccgtggc tgccctggtc acggtccgct tatcggcggg
    19141 agtgggggtg agggagtatg cctgggcata ctcggctgcc gtcaccggct tgccctccat
    19201 caggcgcctg ttgtgctcgc gcttgcggac gatgtccgcc tcggtattgg ctccgatgat
    19261 gcgggcatat tccagctgct gctcccgata tccggccgcc ttgcccatgg cctgctccac
    19321 agcgtgcgta tccgccgtgc agaccctctg cacgagggag gccgtgccga tgacgcgccc
    19381 gtcctgcccg cacacaaaga gctgattgtc atcgtagggg ttgacgtagc cctggtacgt
    19441 gccgtgggcc agaacccgga cggctccgtc aggagtcgcc acgcgggcct cgtaaatcag
    19501 gtcttcgtca cggatggttt tgtcgcgcat ccggatgtag gcgctggcca ccttgatggg
    19561 gcggcccatg tccgtgccca ggatctggca gatgcaccag gggggcagct tgatgagcct
    19621 gttgcccggt ttggcctcct cgcaggccca tgcctcggag gggctcatcc ggcgccggcg
    19681 caccaggtcg gccccggttt cgcaggcggt gcggatgatc tgggcggcca tggacggctc
    19741 cacctcggag gacggcgtcc aggaggcgga agccgagagg cgcagctcct caaccatgaa
    19801 cccgcatcgc tcccagccct ccagcctgtg gtcggtgcgc gtgttgatgt cgctgactat
    19861 cttggtcaac tcgcggctca gttcgtccat ggtcagcatg tagtgcttga gttgaccggc
    19921 tttgtccgcg ggcagcttgt ccatcgcctt gatgagctgc tcctcggcgc gtaccagccc
    19981 gtgcagggtt tccgggggcg tgcggtcgtg gccggtggct cccggcagat ggctgatgcg
    20041 gttgtgctgc aggttgtggt agctttccag cgccgccttg tggcgcgggt tgcccacacc
    20101 ccggccgccg tggccctgct tgagcgtctg gcgcacgcct ccgatgccgg agcggtccac
    20161 cttgatcagg ccgccgctgg catggtagag caggtcttcc agttccttgc tgatagccgc
    20221 cgttccgttt tcgacgacga gcgtcgtccc cttgggcgag tacccgacgg tagctcccca
    20281 cagggccagg atgctgcgca tatcccgctg gttaagatgg atcatcttgc ccgtctcctc
    20341 atcgcggcgg cgtatctttt gcccccacgc cacacgcttg cccgtaaggt actccagcac
    20401 gcccagctgc aggggctgcc ctttgtcccg cccggaaagc accatcaagt caagccagac
    20461 gtcgtcaaag agaaaatgac tgcccagcca gagcccttcg cgggtggcca gtacctgcgc
    20521 cagctggggc gcggcggccc ggatgccctg cttgagcgcc accgtctcca gagattgggg
    20581 agcgagtctc tgcaggttgc gcaggctcca gccggagggg acgcgcggcc agcccggcca
    20641 gtcctcatag ccggggatgg tgtcgcgcct ctccgtccag gtcttgagca ggagctggcg
    20701 ggccgtgggg atgcccccat tgcgctggca acgggtgcag aggccgtgcc agtaggcaag
    20761 gaatttgctg agggtgacgc ggctgcgcgc ctgtatttta cggtagcggc ggtccaccag
    20821 ggagagggga ttgtcgccgt tgttgcgcca gatgtcatac cagcggcgca tagtcaccgg
    20881 ggagatgccg cagagggcgg cggcatcccg gatggcttcc tgctgcccgc catagcaggc
    20941 gctggcctcc ctgatgcgct tgcaggccgc atagagggcc agcactctgt cccgctccac
    21001 cagcggcaga tcgtccatgg tgatattaaa gtccattatc ttaaagtgtt gttgtaccgt
    21061 tgactatgcc atccatcgtg gccctgacgc tctgtacgtc ggcatcggat accttgcggg
    21121 cgtaagcggc cagggaatcg gcaaaaagct ctcgctcctg gaggcgcagc aggtggacgt
    21181 agccctggtc gataaaagtt cccagctgtt tgaggaggca ggctgcgtcc tgctcggcct
    21241 gatgccggcg gcgctccagt tcttccgggg ggaggttgag agcggccagg tcattggcca
    21301 gcttatccat gggggtgagg gcctgcgccc cgtcagggtt gccggagccg gtaaagccca
    21361 ggggcttgac ccggtcgtgc gttgtccgct tgcgggtggt aatcacgccc aggttgatct
    21421 ccatctgtcg gggtgtttcg gctccgtcgg ttactttatc tactaggtcg ggcagctcgg
    21481 agacaggctc accgcatagg cccgcgtcca ggagaattct ctccacatta tctaatcttc
    21541 ctgcggctgt tttataacag cgcatgtagc ggttggacat ctgctgggta aattgaaacc
    21601 gtggtttcaa tttatcgtct ttggcgaaaa attcgcccca ctctccatga ggagtcgcgg
    21661 ccttaagctc ggtcagcagc ttgcccagct tgagtcctgc cgtcacggca ttgcgcccgg
    21721 cgcatgccgc catctctgcc tgcgcctggg cataccggtg caggcggttg gcttcgctga
    21781 cgcccagcgt cacccgcgtt tccgcggtta tcataagctc gtgtttcatt ggttgaaatg
    21841 ttgttgacga ttgatttcct gctgcaggat gtaacggttg agggccaccc tcagcttgac
    21901 cagggccttg tcctgcagct cccagacagc cttgtcggac aatcccaggt aatgggccag
    21961 ttccgggcag gtgagcgggc cgtcgtgagg cacccggtaa tggcgtcgca cttccgggat
    22021 gttccaaatg gcggaccaga tggcccattc ctcggtcgtc atgggcgcgt ccatgtcaac
    22081 gggtgtgtcg tccatcatcg tgtggccttt ccatgactgg caggatggcc gtgcgcgggt
    22141 ccttgaggcc gtcgcgcacc tgctgctcct ccgtatccag ataccatcct cccgccttaa
    22201 ccatccctag gaatgccagg gccatgactg ctatggcggc aatgagattg gcggctctcc
    22261 tcagcatcgt tacctgctcc tccttaatga tgtcagtttt ttgcgcggca accttcggat
    22321 gcggactttc agggcgttgc cccggtctgg cgctcctttg aggacgcggc ggagatgact
    22381 ggctgtcact cctaatactt cggcggcttc ggtcggggtg tatccatgac caagtaacca
    22441 ttgaatgttg atgttctttt cttgtatggt cattgctgtt tgtgttaatt ctgccttatg
    22501 gccgccttta tagggcggtt cgataatcaa attatcgaca atccgaaagt tatggcaagg
    22561 caaaaatcaa taaacggaat atttatttct cgaacaagag aaagaatgaa agataagtcg
    22621 cttaatcaaa gcgatttagc ctatatgtca aacatagctc aaggaacaat ctcggaatac
    22681 ctcaatcctg aaaaagggag gactcctggt gcggctgaac taggaaggat ggctcaggct
    22741 ttaggagtca gcatgggatg gctttgggga ctagagggaa cgtcagagat tgaaactgat
    22801 atttacatcc agaagataca tagattagaa gggcgattac tgtttgcaaa aaaaacatta
    22861 agaggtgcat tggagtattt gcaggaagat gttatggagg atgaaaaatt agcggaaaag
    22921 actctctcta cacagaatga tagattaaga gttgcggaag atgagatagg gtatagtcat
    22981 aatagaaatg aaaacaagaa tgcataaaaa ctacttaatt ataattgctt atgcatgttt
    23041 actaggctta gtggggttag taatattgga gatacacgat gtgcaaacac ggttggcgtg
    23101 cttacaatca aaaattgaaa aacagataaa gaagaaacct gctctatttc aatatgaatg
    23161 ggtcaagatt cgcaagtttc aatggtcaga gaaaaatttt gattacagta cgttaccaca
    23221 tttaacagat aaacaaatag aagaggcaat ggctcggcaa aaggaacata atgatcaatg
    23281 gcacgggcat gagggaattc tggatggttg cgaatatgta gggactctag cagagacgga
    23341 aaactatgtg atcgtgctag taaaaacgaa ggtagaggaa aatgggaatc cctttgctaa
    23401 tgacgcgaat gaatcatggc gtggaaacta aaatagtatg acacagcgca acacactgat
    23461 cataaatttc atatctctaa caaacataac aaatttataa taaatttgtt gtgccgttag
    23521 agaaggacgt ttgagcgagc tatgctcgcg tccgatgacc aacacatata ccatgctgca
    23581 cggggactgc atgtccctga tgtcgaccat gccggaggca tcctacgatg ccgtgattac
    23641 tgatccgccc tacgccagcg gaggcctctc tacggccgcc agggatcgag acccgcgcgt
    23701 caagtaccag ctgtccggaa cccgtaaata ttacccgaca tttgccaatg acaaccgcga
    23761 ccagcgtacc cacctgatgt ggtccgtgcg gtggatggag caggccctgc gcctgacgcg
    23821 ccccggcggc tggctgatgg tatttacgga ctggcgtcaa ctgcccctga cctcggacgc
    23881 cctgcagatt gccggctgga cgtggagagg catcatcccc tgggacaaaa cggaaagctg
    23941 ccgaccccag ctggggctgt accgcaacca ggccgagtac gtcctgaccg ccacgcacgg
    24001 aggatacgac aaatccgtca ggctttgtcc tccgggagtg gtgcgcgagc cgatccgcac
    24061 caaggataag ctccacctga cgggcaagcc ggtccctctg atggagcacc tcatgaccat
    24121 cctgccgtcc ggctcgcgca ttttggaccc gtttgccggt agcggcacca cgctggtggc
    24181 ggcccgcaac aagggacaca cggccgtggg cattgagctg tcgtccgact accaccgtat
    24241 cgccaccgac cgtctcggta tggtacttac agcttgatat ccatcaatac cctgtataac
    24301 aagaccgtta ggaaaaatcc tggcggtctt ttttcataat gtgaatgata taaaaataaa
    24361 tttgatattt tcacaatttt gcatattctg ttttttatat gaaaaaaata ctgcttaagt
    24421 tgactgaaaa tcacgaaatt gaatccatta ctattgacgg acataatgcc ttgaaatggg
    24481 attttatgca gacattgctc gataatatta atgaaacgga tagacacaat gaagtagaag
    24541 ccatacaaga cataaaattg tgggcgcaat tatcagatga ggaagtgaat caaattctac
    24601 catgctctcc atttgaggaa gaagataaaa taattcatct taaaaggcct gctgctgaaa
    24661 cagctcctag tgaattatta atccgcattt atgaagcaat aaaagaaaag gctcccagcg
    24721 ttctaggcta aatatccttt cccgcacgcc ccctgcagca tcccagcagg gggctttttt
    24781 tgccagccgt tagagagggg ctcaacattg tggtaaggtt gaggcatgga cgacaagacg
    24841 ctcaaacgct gggcctccgg gccgctctgt accattgccc aggcagcggc cctgctgggg
    24901 gtatcctcgc agacagtccg gcgcatggtg ctgcatggcc atctcatcgc gtggcgtccc
    24961 aatccgtcgg gccaaaagag gctgctctac cgccgccagg tggaggattt ggcggcgcat
    25021 agccagagcc gtgccatccg gcaggcccgc ctgatgcagg atacattcga ttttttttaa
    25081 gccaaatgca acaaatgcgc caaacgcgcc aaacgcaaca gagggttgcc ggaaatgggc
    25141 taaggtgccc tcatgaacaa caacccgctt cctgttgata atactggtgc gcatgacgag
    25201 atcccggctc cgggtggagc cgggaatacc gtcaccggaa aaacgccttt ttatctgtcc
    25261 aaatactttt ggaccaacct ggcagccttg ctggctctga ttatccccga tgtacgggcc
    25321 tggctggaca gtaaccccat tgaatttttc agcgcccttg gcgcggtcaa cattctgctg
    25381 cagtttataa gcggcggcaa ataccagctt gccggagagg atggccagag cggccagtcc
    25441 gccaacccgg ccggagccct cccgccatcg cctgttaccg gggagggctc cacatccccc
    25501 gtggaggacc ggagtcagga tcctgatcag gatccgtcgt ccataccccc cagctccggc
    25561 tcctcctcac ccttatccgg gacctcccgc ctgatggtcg tcctgggagc cttgatgctc
    25621 ctgctgggcg gatcctgcag tacggacgca gaccctgtgg ccgccagcgt cagcctgagc
    25681 gacgggcagg tgatagtcat ccgtggaggc acctctctgg tagtggaccg cacggagcac
    25741 aagctgctct ggtcacaggc cgccccggag gcagttgtgg ctccggtagt acaagcctca
    25801 tccaagtaac cgacttttaa ctgtcagcta tcatgattta cgcagcatta caatccgaca
    25861 cgctgtcctg gcagcgctcc ctgaaattcg ccggttttta ccggggccgg cttgacggcc
    25921 tgacaggccc gctgacccgc gaggcggcgg cccagtggcg cgagagccat caacagctcc
    25981 agacccgcta cggccggctg gacaagcgca cggaggacaa cctgcttacc ctccagcccc
    26041 tggccgcgct caaggtgcgc cagatgatga cggccctgcg cggtcttgcc gactggcggc
    26101 tcatctgcgg actccgcacc tacgacgagc aggacaagct ctacgccaag cgtcccaagg
    26161 tgaccaacgc caggggagga cagtccatgc acaattttgg cattgcgggc gacgtctgcc
    26221 tgtttgtcga cggcaaggac gtatggacgc ccagcgaggg agcgaattcc atctacaagc
    26281 ccgtggcggc cctggcccat aagctggggc tggtatgggg cggggatttc aagtccattt
    26341 acgaccccgg acatgtccag ctgggcgagc tgtccacggc caccctccat cacgcctaca
    26401 ccaccgggtc cgccacgctg gcccaactgc tccaatcatg atgctcaacc tgatagcaga
    26461 cgccgccgca ggcatctcgc cggaggcagt aggcaccatc ctcggcgttg ctctcggctc
    26521 gtccgggaca tggtgggtgg tcaagggccg caaggctccg cagcaatccg acgatcccca
    26581 gcgcttcttg atggaggaca agtacgcgac ccgtgaggag gtggcggagc tcaaagccct
    26641 ccacgccaag accaccgacg acatgcacaa gcgcctcaac agtatcacca tcaagctcaa
    26701 cgagatgtcc ggcaccctta aactcatgat tgacatcctc aagacccgta aaaccttatg
    26761 accacccgcg ccaatatcaa gattaccatc ctgcaggtcc tcgaccgact gcccgctgcc
    26821 tatacccagc gcgtgtctgc tctccgggcc gaggtctccc tggacatgtc tccgagccca
    26881 ggcactgccg atatcgacct ggccatccgg gaactggagg ccctgcgcct gatcacgtcc
    26941 accacctgcc ttatcaccgg cgagcgcaag tacgccatca ccgacgccgg ccgcgtccag
    27001 cttacccaga tatgaggtaa cagttaacag tattaacgcc atgctccgca aacccagacc
    27061 agacagtgtg atcggctccc agttgccccc catcatcaag gatgacgtgg acgctatgtt
    27121 gttttccggc gcgtcttaca aagatgtcca ggaacgcctg gccgaggacg gtgtgacact
    27181 gagccaggag gccatccgcc gctactacca ctcccagatc ctgccggcgc gtctggcccg
    27241 gcaaaacaag acggccgagg aactcaacaa gatctccgtg gacggagtgg acgaagccac
    27301 catgagggcc atccgctccg ccgccctgga cctggccgcc tccccgtcct gcgatcccaa
    27361 ggcgctgagc atcctggtca acctcatcct caaggcagag cagctggagc aggacaagcg
    27421 ccgcctcaag atgctggagg ccaaggccgc ccaggcagac gccgccagac aggtcacgca
    27481 atcaaccctc accccggagg agcgcgacgc caggatgcgc agcatcttcg gctgttagac
    27541 agtaactcac ccactcccct tcatgtcttc cctgatcaca acgccgctgg agctgctgct
    27601 gccttaccag gcacggtggg tggcggacga gagccgcttt aaggccggca tctggtcccg
    27661 ccagtccggc aaggattttt ctacggcggc ggaggcggta agggatgcga tgctgcgggc
    27721 caagaccacc tggatgatcg cggccccgtc cgagcgccag gccatggaat cgctggccaa
    27781 gtgtaaggag tgggccgagg ctttttccct tgccctggcc gccgaggaaa tcgaacgaca
    27841 ggacggcccc aacaccctgc tcaagtccgg ctccatcaca tttgccaacg gctcccgcat
    27901 cttggccgtg cccggcaggc ccgataccgt gcgaggcttt agcgccaatc tcgtcctgac
    27961 ggaattcgcg ttttttgagg atcccgacgc cacctggaga gccgtgttgc cctccatcac
    28021 caatcccctc cggggcggcg agaaaaaagt ccggcttatc accacgccca acggcaagac
    28081 gggccgcggc gccaggacat acaagatcat ctccgacaat ttgctccaac ccgtggaggg
    28141 ccgcaagcag cactggtcct gccatgtggt gacgattgcc aaggccgtgg aggacggact
    28201 gcccatcgac attgacgagt tgagagaatc cctggacgac cccatcggct gggcgcagga
    28261 gtacatgtgc gaattcctgg attcctccaa cgtgctgctg ccctacgacc tgatcgccac
    28321 ggcggagtcc gccagcgcca cggtctcctg cgacccggcc atttacctgg gaggcaagct
    28381 ggacctgcgc ctgggcatcg actttggccg ctccaacgac ccgacggtct gctggacatt
    28441 ggagcgggtg ggtgacgtgc tggtcacccg cgaggtgctg gtgctgcgca acatgtccgt
    28501 gccggaccag atggaggtgc tgcgccaccg catcaaggcg gcgcgccggg tgtgctacga
    28561 ctacacgggc gtaggcatcg gtatgggcga tgtcctggtc aaggaattca agcgctggca
    28621 tccggagggt cacgagtttg gccggatcga gctctgcacc tttacgccgg cctttaagcg
    28681 tctcatcttc ccacgcctcc gccaggcctt tgaggctccg acccgcgtcc gcatcccgat
    28741 tgacgtggag attcgcgagg acctgcacgc catgcagcag atattcagag gcacggacta
    28801 tacctacgag gccccgcaca ccagggaagg acactccgac cggtgtacgg ccctggctct
    28861 ggccctgcgc gcggccgacg gccacgtgca gcaccatctc ccggcgccag gcagcggacg
    28921 catcatcacc ggcgctggac tgtttggcgg ccgctcccac ggctccctgt tcggaggccg
    28981 cccctccctc aaccatctac cgtcagcagc gtaactcatg atcaagcgac tttacaacta
    29041 catcctccac cgcaggctcc atgcagaggg cgtgcagcgg gcgcttaccg cgccggacaa
    29101 ggcatcccag gacagcaagc cggccatgtt tgccgcactc gccctggccg agctggacaa
    29161 ggtacgccgc cgccagattg agcgcgtgtg cccgctggac tacctgagcg tggataccat
    29221 acggcgctgc ctgcaggact gccagctggg agcctatgcc gagcagcagt ggatctggga
    29281 acagatggag cgctacgacg ccatgctgat gacctgtatt accaagcggg acgacgctct
    29341 gagcaagtac gactggtcta tcacggtcaa gccggacctg gacgaccgcg acagcctgct
    29401 ggccgagagc cagcagcgca ccattaccga cctgtgcaac gccatcgtta acatggacga
    29461 ggccatcacc gccctgtccc aggcgtcccg ccgccattac aaattcctgc agccctacgc
    29521 cgacggcgat ggcctgcatc tgctgcccgt cgacaactgg ctgatgtgcc gcgacggtta
    29581 ccgtggaccc tggggataca acccgaatgc ccagtttagc cgctaccggg gggagccgtt
    29641 gcccgtgcct ctggaggacc tcatcctgcg cctgcatccc cggcccatcg acatgcccgc
    29701 ccagatgctg gtgcttaacc gtagcacgac gctggcccag tgggacgtgt tcttggagag
    29761 gctgggtacg ccgccggcat tttttgtgct tcctcctgac tgctccgagg agctgcgtca
    29821 ggagtacatc aagatgtcgg ccatgtgcta ctcggcggcc accggcgtca tcgaccacga
    29881 ctccgatatc aagtccatcc cgatatccca gacgagcgtc gacctgtttg accgccgtta
    29941 caaggtagcc accgaggaga tcgccatgct gaccaccgcc ggcaagctca cagtcatgac
    30001 cgagtccggc tccggcaccc tggccggcaa cgcccaggct gacggcttta aggcctgggc
    30061 ggccggcgaa gccgaccata ttgcctccgt gctgacggcc cagctggtca accgggtgct
    30121 cgacgagtac caccccggcc agcctcacct ggtgaatttt acgctctcct gcgtagacaa
    30181 gaccacgccg gagaaagaaa tcgccaacgc cgccgctctg cgcgccgccg gctacgacat
    30241 cgacgacgcc gaggtcagcg agcgcaccgg ctggcaggtg accgccggag tctcctcctc
    30301 cgagctctac gccatcagga ccgccggcta cgcgccggag cagcgggcca tggagaatgg
    30361 cggcaaacgc tcccaggaga cgccctacac gcttaactcc cgccgccgcg acgccatcac
    30421 gacactggcc ctccaccgcg gcaccacgct ctgggagccg gcgcgccgcc gcctggagga
    30481 ggtggtcgcc caccgcctgc aagatatcga cgagcgcctg gaacgggtca cgctggagct
    30541 gcttcccctc tccccggatg agcaggcccg tctcaaggat gcgctgcagg tccccggaga
    30601 ggaggagatc gtctccaccg ccctccagat tgcccgacgc ctccaggagg cccgtgacga
    30661 ggggaaacgc cgcgcggcgg ccgttgaccc gtctctggcc acattcggcc ctgcacgacc
    30721 cctgcacggc gcaaattcag ccccatccca cgcatgagca actaccgaga cagatacatc
    30781 gcccgcggca tcccggaatt cgacccggac gcctgcgctc cataccccat tggacaggtc
    30841 ccgcaatcag gctggttcct cattgagccg gccggcacct acaccatccc ggtgcccgac
    30901 acctccatcc cgcctgccaa gcgctgggac gtggacgagg tcatcgacaa ggcggcgctg
    30961 caggccatct gcgaggccta cgacccggct atcaacggcg gcaacggcat ccaggtcaac
    31021 aacgaccacc tgcatctgcg caccaccggc gacaacccgg ccctgggctg gtgcagggcg
    31081 ctggactacg gatgggtcgg cggccgcctc taccaggccg cctacatctc ctgggtcaag
    31141 gatgcccacc acgacctcaa ccagggcaag tactgggcat tcagcaccga gtacaagctg
    31201 gccgactaca aacgcgtcta ccacaacggc tacagcccca cgcgcctgtc cggactggcc
    31261 gtcaccaaca acccggacca cgaggcccag ccgggcatca tcctccaatc cgccgcgggc
    31321 gacgtggtcg tccacagccg cagcgcctcc atcctccaat ccacaaccat gagtacaaaa
    31381 acagcaacac aacggatcct gcactccgag ggcaatcccg cccccgaaga tgaggaaaag
    31441 aagcagcaag aaatcaacgc caacaacgac aatccccctc ctgccaccac agaagaggaa
    31501 aagaaagacg aaaccagcgc caacaacgac accgaccaaa aggacgaaac cagctgcaac
    31561 tcggacgacg agggctggct gggcctcgtc aacaaaatag ccggggtgtg cggcctgccc
    31621 gacacggcca ccggagacga catcctcaag tacgtcaccg acctcaagac cgactttgac
    31681 ctcctcaagc agcaggcaac agagtccaac ggaggcaccc aggcccacag cagggctccc
    31741 ctcacgcgcc agctgcacag caaccgggga ggccggcgca tggaccgcga cgtcactccc
    31801 gccggggtgg tcatccaccg cacgccggag ggcaaggccg tcaaggtgcc gcaatccgac
    31861 atcgacctgg tgacccactg ccgccaggcc gtggacgccg agatcgtcag acacggccgc
    31921 cagctcaccc ctggcgagta cgaccgcgcc tggtcacggg cagcggagga attcgcctcc
    31981 gcgcgccgca agtaactcca atctcaacac ctaactctaa ttaaaatgat tatcaagcaa
    32041 acacccgtag aacgccgtct ctgggcgtcg ggcgccatcg gcaccgacaa gagcgagggc
    32101 aagctggtca aggcatctgc cgacggcaaa accatgtctc tgctgacctc cgcctcggat
    32161 atcccggacg gcgttgtcag caaccctgac gggcgcgacg gagccgacgg caacggcggc
    32221 gatctggtcc gtatgagcca tccgggcatt gtccaggtcc gcctcaacga cactcccggc
    32281 accatcgagg acggcaccga cctggtggcc tgcgccgacg ctaccgtcaa ggcggccacc
    32341 ggcgcggccg gcgaggtcgt cgtggccaaa tccgtagccc ccaacaccag cggccaggga
    32401 ggctgcctcc atgacgccat cctcgtcgcc aggccggcag ccactccgga agccgcttcc
    32461 gacaattcct gaccattaac tcttacctct taacgactac atagcatgag tacatccgca
    32521 acctacgccg tcaacctgcc gctgaccaac tacatcatcg gctggtacgg cacccagacc
    32581 cacgacccgg cacgcttcct ggctcccggc gtcaaggccc ccggcctgct gaccacctac
    32641 aagcgctatc tgcgccagga cgcttttgcc gcctcggaca cccgcaggcc catgtacgac
    32701 tccccccgca ctatcgacat ccgcggcgag gacgtcccgg tgatgctgga ggaacacgcc
    32761 ctcaagatcg gcattgacga ccgcgagctg ctcggagcgg ttgacgcgga agtttaccgc
    32821 accagcctgc gccaggccaa aacccgcgcg ctggcccgcc gcatgctcat ctcccacaat
    32881 aaggaggtgt ttgactacgc caactccgtc atccccggca tcacgtcggt ggacggcatc
    32941 acggaggcca acaaatggag cgaccgcacc aagcccgtgg tcagcatcct gaccagcctc
    33001 atcaacaaat tcgccgtcaa caacggcgtc tatcccaacc gcatcctgac cacgcgcgac
    33061 gtctgggcgg acatccaggc caacacggaa gtccagacca tgatgggcga gatgggccgc
    33121 aaggtcctca cgccggagac gctgctggag ctcatcggcc tgcagggcga cgacatcccg
    33181 ccggtcaggg tcatgcgcac cattgcctcc tacaatcccg gaggcacggg aggcgcggaa
    33241 gtggacaacg tcaatatcgt gggcagcaac atctatctgt tttacgccga cgacaatccg
    33301 tccctggacg acatatccgc gctcaagacg ctcaacctcg ccggcgacga catgtacagc
    33361 acggtggaaa cctatcgaga tgaagacatt tccacggaat ggctgcgcgt gcgcggccat
    33421 cacaaggtcg tgttcgccgc tccctcggcc atgatgcgca tgcagatcgc ctgatgcagg
    33481 atggagtgtg gagagccggg agttgtgatg ctcccgcctc catgctccct ctcaactctc
    33541 cactcttaac actcaatttt tcatcaagat gacagccaag aaatcaacca agaccaacaa
    33601 acctgcccag gagatggaca ccaccactcc tgatcaggac aaggccacgc cgaccgtgga
    33661 ggacaccgct gccgaggcat ccgcatcgga gcctgcagcc aatcccgcca atcaaccagc
    33721 cgtagagccc gccactgact gccccatggg cgacagcgac tccggggagg caggagacga
    33781 tcccagcccc aacatgacca gtcacgagta cgatgacctg catggtctga cctccaacat
    33841 ccatgacgac agcgactccg gggaggcagg ggacgatccc ggccccaaca tgaccagtca
    33901 cgagtacgat gacctgcatg gtctgacctc caacgtccat gacgacagcg tctccggaga
    33961 gcagcctccc ctcgccatcc tgaccgccaa gcggctcggc ctgtcgccca gacagcacat
    34021 cacatccatc atcgccggag gcgtgatcag cggcatcctg gcccggacca aaccctacga
    34081 gatgctcgac ctgcgcaagg cggccgaggc catcggcatg tgcgacgcca tcgtcgagat
    34141 gatcctgcac ctggacgatg ccgccccgga ggccccggac gagcaacccg tctcctgact
    34201 attaaccgtt gcctgccatg aatcactgga tcaccttgac ggcggatatg ctcctggagg
    34261 ttatcgacca agccgagctg gatgccatca cggccgccga tacggacggt tctgttgttg
    34321 acggcatcat ccaggacgtg acggcctccg tgcgcgaggc tatcgctgcc aaccccgcca
    34381 atgtgatgga cctcacgagg gacaccatcc cccgcaccct caggccggag gctctggaca
    34441 tgattgcctg gcggctgctc aagcggtttg ccgtggcagt cagtgagtcc cgcgacaagg
    34501 ccgcgacttc cgcccgcgag cgtctggagg cggtccgggc cggcacccac cgggtgatcg
    34561 gtccggacgg ccgcatgccc gtgcctcccg gcaagcgtcc ttatgtccag ggaccgcgtc
    34621 ccgcctacgg ttccggagcg cccggattat ttccttcccc ccggcggagg aggtaacagt
    34681 aactcagcct ttcccactca gcaatgccct ctccctcgga ttacatgcga gccaaaatca
    34741 cggttcctgc tgccggcatg tcgtcggcgg actgggacgg cgtggatccg gacatccggg
    34801 agcgctccta ttggacggcc agggagggat gctatgcccg cgtgcaggga ttccgcgacc
    34861 gctgccaggg gattgtggac ggcaacctgt ccgaggcgga cgctctgcgc gaggtccgcg
    34921 cgatgctgcg cgccaccggc taccagccgg agccgggcac ggagggtact atccaggacc
    34981 tcaactccga cgcccgccag cgtctcatcc tggacaccaa cgtcgccatg gtccgggaac
    35041 gggcctaccg ggactccatg ctggggtcgc tggcctatcc cgcccagcgc ctggtgcgta
    35101 tccgctacag ccgccagccc agggactggg acgcccgctg gagggaggcg gcggccgccg
    35161 tcaactacga gggcgtggct accgacggct cccacatcgc gctgctgacc tcgcccatct
    35221 ggcgcaagct cagccggttt gatctggatt accctccctt tgattttaac agcggcatgg
    35281 gggtggatcc ggtcgactac gaggaagctc gacgccacgg cctcacgatt ccggaggcga
    35341 cgctggaggg agctgacggc gagtcgctca acgccagcct ggaggcgtcc attgccagga
    35401 tggacggcga cctgcagcag gcatttgtca atgcgctgga agactgcgtg gaggtggaag
    35461 gagaccgcgt ctactacacc gaccccaacg gcacccgtcc ggtgcattgg agcgaggcgg
    35521 gcaaggttat ctgcgggcag cgtcctcccg tcatcccgga cacccaggcc agagcgatgg
    35581 ttaaattcgt tgaggatcag cgccagtttg accgggcgca gcgaggcgtc cagggctatg
    35641 ccacccagga agagtgggac gccctgtaca acgcggtcac ccggatccag cccaccgacg
    35701 tcaaggagag cggcacgctc tatcgcggca tgtcgatccc ccccaatgat gttgacaggt
    35761 tcctgtcccg catcaggcgc gacggctacc aggcgctccc caccaagatg gtggacagct
    35821 ggtcgcgcgc ggaggagacg gcgcagcggt ttgcgggtgg cggcaaaccg ggcaacgagc
    35881 gggtcatcct ggtcaacgag gactaccggt ccggccaccg catcgacgcc gtcgtccgcg
    35941 ccctccagga gcaaaggcgt cttgtcaaca agaccgacaa acatccccac accaatgagt
    36001 cggaagtctt gttcctgcag gccgcgcgcc accaggtcaa caaaatcgtc cgggggaggg
    36061 acggcacacc cacttacgtc tatgtcagcg agcagtaata acagatcagc ccttgcggca
    36121 cttgcgccgg ttctggcggc cgttgccatg accattagcc ggccggtccg taccgcacca
    36181 gcagggcaca tgctcgccgc gctcaacagc ccggcgctga tactgcgtca gctctgtcca
    36241 tcgcagcttg cgcggtgcgg gagaatcctg ctctccagat cggccgggag cgcgcaggta
    36301 ctgcagccac agcgccatct cgcgcttgcc gatcttgccg tccattccgc tcatgtcccc
    36361 cttatgcccc aaccctcaac ccttgtcaac tcatgatcag gttacacgtc gatgtgattg
    36421 gcctccaacg gctccctctg gctcccgcta tccgcgcgga gatggtggcc gatgtggcca
    36481 gggccgcccg ccaggcggtg agggacagct tgcaggccat gatcgaccgc acccagtccc
    36541 agggattctg gggcaaggcc aagcagagcg tcaaccctcc cgtcatccgg gacaacaagg
    36601 ccaccatcga catcacccac acaggcgtac gcctgcaatg gctgggaggc accgtcagac
    36661 ccaccggccg caggtcggag gtgaccggcc gtcccatcag gagcctgctc atcccgttta
    36721 aggattcacc cctgcgccgg cgctccctgg ccagcctcca cctcccggag gaggaggtca
    36781 tggtcctggg cgacgtcgat accggcaacg ccatcctggc ccgcgtcagg cagcgcaagc
    36841 gccgcaacag ggacggccat taccaggacg tcaccccgct gggagccctg gtcaagtccg
    36901 ccaccatccc ggcccatccg gaggtgatgc cctcccgcga acagatgcgc gagtatgccg
    36961 tgcgcgccgc cacgctggcg ctcaaccgcc tcctggccca ggccgacggc aactccactc
    37021 tccactctta actcactaat ccccatacaa tggataaaga ctacaaactc gccgagcatc
    37081 tcatcgccca cctgcagcag gacgacgtcc tggctccgat ggtgtgccct accgtgtggg
    37141 acgagcagga gcagatcgac gccatcaacc gcgcggccat gggcaggccc ggcagcgtcg
    37201 ccgtcactcc ggcaggctac gtccctctct tagagatggg cgtcaatgct ccgatggtgc
    37261 gcatgcacgc cgtgctggcc gtgagctgtt ttgcgcggga tgccggaatg ccgggcggcg
    37321 ttcctcccct ccgttgcctg tccggcatgg tggggcggac tctgcatgcc gtccgcctct
    37381 gggatccggt tgtcgaccgc gtctgctacg acactccctc cgtagcctcc gtcgaggact
    37441 acgacatgac taaaagcagg ctgaccggct tccggggccg ggccgtcatc ctctacgctc
    37501 ccgtcaactt ttaatttttt cccatcctca cctacaacac acaaccatgg ctaagaccaa
    37561 tacaaccaac aataccgata cgaccggcac gactccgcag gctcccgccc aggaggagac
    37621 gctggtgtta gtccgcgtga ccaagacggg cacgctcatc aacggcgctt acgggcgtgc
    37681 cggcgctacc gccagagtga cagcctccca ggccaggtct ctggaggcgg ccgggctggc
    37741 cgtcatcgtc ggcgtgtaac tgccaaccat taacccatca ccaatcactg atatgtccag
    37801 atttattatc cccggcctga tcatcggctc caaggtcagc atcgccaaat tcggagccgt
    37861 catcggcaac gacgaaccgt ccaagaccgt gtccgcggac tggctgcccg tccccccgac
    37921 ggcggaagcc cccggcccct ggctctatat gggcaggata cgcaccagca accctcagat
    37981 tgagaccaag acgggcgaga ttgagggcac caacgacggc ggcacttacg aaaccgagga
    38041 gctgcagctg accaccaagc gcaaattcct gtttgccagc aactacatca ccccggaatt
    38101 cctgcagctc tcctttggcc ttgcccagga ctggggcacg gagcaggtgg tctttggctc
    38161 cggctctccg cagatcgacg tctatgtcta taccgagtgg acggacgcct accgcgacgg
    38221 ggccagaatc atgagcgcct gcatgcaggg ccgcctgcgc ctggtcaacc cggccaaggc
    38281 tgcctccgat ccgtccctgg cagaattcga gctcagcgtc atctacaatc cgctctgcaa
    38341 gctgacccct gacgaggact acgcgggagc ataatcccgt cctccccccg cggcggcgtc
    38401 ctttccgccg cggggaaaca cctcttacgc ctaatcactg atacctgtta tgcaactgct
    38461 gattgatctg gccaccatgg ccgtcatgtt ccccggcggc atcccggcca ccgacttgtc
    38521 cctggtgcgc ggcgacaaga ttcccctgcg cgtcacgctg ctggatgagg gcgctccggt
    38581 gactccctcc ggcgtcagac cggcgctggc cgtcaagacc gccctgggag acgagaccct
    38641 ggtgctggcc gccacc
//
LOCUS       DAWEMX010000007        36024 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_477_length_36024_cov_11.651033, whole genome shotgun sequence.
ACCESSION   DAWEMX010000007 DAWEMX010000000
VERSION     DAWEMX010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 36024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 36024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36024
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_36024_cov_11.651033"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    316..325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2744..2753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7395..7404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15774..15783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16982..16991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19692..19701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20569..20578
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8002 a        11106 c         9215 g         7631 t
ORIGIN      
        1 ttattcaagc aagttcaaga attttggtaa aatcaatgtg taaacagtcg tcgacactta
       61 tggttacttt gcactcgcag tatatgacac cgataaaccc cattcttcca agtaataaca
      121 tttctatagt ccctcatctg ttagcgttcc cattttagga aaacaaagat ttattatcac
      181 agaaccattc cctgttactg tttcataatg agggacagaa acagcattga agaaaaaaat
      241 cttcgtaaaa tattaaaaat tagacgttta gaattaaatt taactcaacg agatttgggg
      301 aaacttttaa aaactnnnnn nnnnntcgtc aacaaatacg aagtaggaga acgctacctc
      361 acctttacgg aagtcattga tatttgtaaa aatctaaaga tagacattca taaccttctg
      421 gatgatgtaa tgaagccccc ttccaatgaa tgacaggacc ggcagaacag cttgttccct
      481 tgagcccggc atccgaatcc cccgaccgtt ttggcgtgga actaagcctg ggaacagcgt
      541 tgcagaaaaa attcccgcaa aaaaaatggg ccatcatcaa gcatgcccgg agcggaagca
      601 accttttccg gcaatggaac ccaggaaaaa cttctcagga taagcagggg gaagaatacg
      661 tcaaattgct ccgtaccgtc cgcaacggaa tggaagccct gaaaaagcag ggacatgctc
      721 ctgtcctgaa agccatggtg tggcaacagg gggaaggaga cgcaagggac atttccggca
      781 tcaaaaacgc tctgtcttac ggcgccaacc tgaataacct gattaaacgc atcagagcgg
      841 atctggaagc ccccggtctg gcctttatct acggcagcgt tcttcccgtg cccgctccgg
      901 cccgttttcc gggcagggaa aaagtgcggc aggggcaaaa ggatgtggcg gaagaatccc
      961 gtacctccct ctccgtcaac aatgtgctct atgtccccgc agacgatctc cagctgcgca
     1021 gcatggactt ccgcaccccc tatcccacgg ataccgtcca tctgggcacg catggagttc
     1081 tagccctggg agaacgcttt gcctccgcat tggaaaagct ctggagacaa aaaaactgac
     1141 acccctgctc tttttttctt ccccactttc tccagcccca tccggaaaac caataatgtt
     1201 tgacatccct ccgggctgcg gatagggttg actatcattt cattccctgc ctccaactcc
     1261 gttgaaaagg gacgcaacac aactctctca tccattattg cccatgaacc cattgaaagg
     1321 agcatgcatc atcggccagt ccggtggccc caccgccgtc atcaacgcca gcgcactggg
     1381 agccattcag tccgccctgc aaagcgaaca ggtcacgcgc gtgctgggag ccgccaacgg
     1441 gattgaggga gtgctcaaag aacgcctgtt cgatatggcc cgggaagacc ccgcagaatt
     1501 ggaattgctc aaatataccc cggcatctgc cctgggttcc tgccgctaca agatggccgc
     1561 cccgtccgtg gatgatacgg attaccgccg tctgctggaa attttccgaa aatacgacgt
     1621 acgctacttt ttctacaacg gaggcaacga ttccatggat acctgcaaca agatatccaa
     1681 attcatgcag cagtccggtt acgaatgccg cgtcatcgga atccccaaga ccattgacaa
     1741 tgaccttcac ggcacggacc actgccccgg tttcggctct gccgccaaat ttatcgctac
     1801 ctcctgcatg gaagttcacc aggacctccg cgtatacgac aagggacgcg tcaccattgt
     1861 ggaaatcatg ggtcgtcacg ccggctggct ggccggatcc gcagccctgg ccacgtatgc
     1921 gggagcaggt ccggacttgg tttacctgcc ggaagttccc ttccgcatgg aggaattctg
     1981 gcaggatgtg gaccggattt acaggcagaa cggaagttgc atggtcgccg tctcggaagg
     2041 cgtgcaatat gcagacggcc gctttgttgc ggaatccgga gacagggatg tcttcggcca
     2101 tacgcagctg ggaggcctgg gagccatgct tgcggaatcc gtcaagcggc aaaccggggc
     2161 caaagtcaga agcattgaac tctcactcct ccagcgctgc gcctcccatg tcgcctccaa
     2221 gacggatatc gatgaagcgt atatggcggg aaaagccgcc gtggaagccg ccgtttccgg
     2281 ccagacggat aaaatggtag cctttgaacg aatcacggag aacggcattt attcctgcaa
     2341 gactaaattg ataggtctgg aagaagtggc caatgtggaa aaacttgttc cccgcgaatg
     2401 gatcaacgcc cgcggcaatg gcgtggaaca gccgttcatt gattacgctc ttcccctgat
     2461 ccagggagaa acggccatgc aaaaggaatg ttccctgccc cgcttcgcga aactcaggaa
     2521 agtgccggca aagccggaac agcggtagca gttttttcag ggaagcgttt ttcctcacca
     2581 acccggccgg agctgcctgc cccacgctct tgcaggtaaa catccggccg gggctcttct
     2641 ccccgcccgg tatcttgaaa aggatgaagg gaaagacggc gcagcggttt cattcataaa
     2701 cgggaaaaaa catctccatg gcaggaatac tctttttccc ttannnnnnn nnnactgaat
     2761 gaaaatggag tcggtttttt cccatatttt cttatgggac aatgcttttt aaacatatca
     2821 aaaacaaata gaaagaaaca ggtaaacaaa aaatcttgca ggcaaggatg gaaaaaggct
     2881 aaaataataa aggagttcaa cattcggctt ctatcgtttc gcggcgcaaa actgcttgtt
     2941 tccggatatt tttcaggaaa ccggcatgga atcgctcccc gcttgcctat ccaagttttt
     3001 cccgcatgcc atgctcgatt atctgtttca acatttttat tcaacgctgg ccatcttctc
     3061 tttttgcatt gccatcctga ttctgagagc cctcggcaaa cgcagcagtt tctggattcc
     3121 cgtctccacg gcccctttag gttgcataac cttcctggcc gtctttctcc tgctgacggc
     3181 ggtcattatt ggaacattcg gctttttcac cacaattccc atagcggaaa atctggcttt
     3241 tgccctggct tggctggcag ccatggctgc cgctctggcc gttatttcac gcctgaccgg
     3301 ctattacctg cagcaggaac catccgcagg caggcattat ccggaggaat atggaaaaat
     3361 gatgtcagcc cagctccgga acaaaggcat cctgctattc gccatcatcg gcatcatcag
     3421 ctgcgccatt gacatcatta cgcttccctt cccgattccg gaactccctc cggggtcacc
     3481 cctgtctgcc gccaccatga ttcccctgcc gccggaagta ccttttgaag tatgcatctc
     3541 ctcccgtgtc acgaccaggt tttccggaga atgcgatttt gacgcgaccg tccggaaaaa
     3601 gggtggcggc aaacacgttt tccccgccat tttgatcaat cccgggggga gaagcgccgt
     3661 taccatttac aggctgtggc cggaggacaa gcatcatcag ggcagcctga ttttcactaa
     3721 cggaatacaa agctgcctgc ttaatatgga taccatgcag atgaagtccc tgaacggccc
     3781 cgcttccaat tatcctcctc cctccagtga agacctgtcc ggagaagata tgaatctccg
     3841 cctggaaacg gtaggactgt accgggggct ttccttcgta cgcggagcgg aacactcggc
     3901 tacaacgcgt tcttatattc tgaaaattct ggaaaacatg caggagcggg cggaacgcct
     3961 caaagaagcc attgacagga actctcccga atccttaacc aagtaacaag cgggtttact
     4021 gttgactcag ccaggacgct tcatccccgg cggcgaattt ttcaactact ccttttcacc
     4081 gtgccggatg ccctgggcat ccagaatttt ttcatacccc gtcaaacgcc tccggaaatc
     4141 ctgaaaattc ttatcctcct tccgggtcca ccccgtctcc gcataagcgg caatacgggg
     4201 aaatacatga cggtgcagtt tctccacgtc cgcaatccat tccgtccaga cctggcagcc
     4261 cagcccgatg acattcttct gctgctccgg gttcaatccc gcaaaaacgg gctcaaaaga
     4321 ataggacctg gacagaggga gcttgtcata tcctttgtta atgtaagtca tgtgggaagt
     4381 ggaattcacc acctggcggc cttccctgat cgccttggcg gcaattttat cggacccata
     4441 ccagaaatga atgatagcat ttgcatccag cttgccgagc gcagctcccc ggccgccgtc
     4501 attgtgcacg tcggacccca gtatttcatt ccaccccatc atccggcgcc ccctcccagc
     4561 cagcatgccg gaaatccgat tcgtaaacat gatttgaagg tcgggaaaag tcttaatgcc
     4621 ttttttcttc atgaacgcct caatgtgggg cacgccttcc cagtactttt tgtaccgcac
     4681 ctcgtcaccg ccgatgtgaa tgatacctcc gggaaagagg gaagccagct ctgtcatgat
     4741 gtctcccagg aactggtagg tacgttcgct ggtgggatca aacgccgctc cgtcattgaa
     4801 actcacgggc acttccgcga gaggcctggc ggacaatttc aactccggat aggaaacgat
     4861 ggcggcggcg gaatggcccg gaatatctat ttcaggaaca atcgttatat tcctgtcggc
     4921 cgcataccgg acaatgcgcc tgacctgttc ctgcgtataa aagccttcat ggggcctgcc
     4981 ttcatacttg ccgcttcccc acgtctctat ctccgtatct ctcctcttgc ctcccacgga
     5041 tgtcagtttg ggatattttt taatctggat gcgccatccg gcatcatccg acaaatgcca
     5101 gtgcaggaca ttcattttca ggagccccat ttggtcaatc agcctcttca cctcctcctc
     5161 tccaaaaaaa tgccgtgctt catccagcat gaaggctcgc caggcaaaac ggggataatc
     5221 caatatggaa aaaggcttct ccgctacatt ccaacttacg ctctttttgt ccgcatcatt
     5281 gttgaaaaag gcggcaggca gcatctgcgc cagactttgc cccgcataat aaactccttt
     5341 gggggtcgcg gcgctgatga cgatatttcc gggagccgct tccaaacggt atgcctccgg
     5401 cccgtactgc ttcagggcag cgtccttcac aacgcggatt attcccgtac cgccagcccc
     5461 gtccagagcc aatccggttc cgcgtgacaa aaactgctgc aacaagtgag cctggcgccg
     5521 gaactccccg tccttctcct tgcatataat gcgcattcct tcatccagct tcacgggcgt
     5581 tgccgccccc ttggcctcta ccttcacgga cacgggccgg ggtattatct gatcctgagc
     5641 attcacggcc gtgccgcata agccggaaaa cagaaaaaga aacacatggg aaacatgtct
     5701 gaacatcatg ttcaatatac actgtttttc ccaggaatct ttccttttct ccccagtttc
     5761 tcttccaccc gtcaaaaccg gggtgcaaca tcctctccac gaagtgccgt cgtgccgggc
     5821 cgcctgaaat gccagcaggc tccccttgcc ttccttttac cggcggcgta tgatgggcat
     5881 catgaaaaaa gacgacatcc cgggcctgtt ctccgccttg gcggaccgcc ctctggcgga
     5941 ccgcctgagg ccgtccgcac tggaagacat catgggccag gatcatctgc tgggggaagg
     6001 aggacctctc cggctcatgg cggactccgg caaaatgacc agctttatcc tatgggggcc
     6061 gcccggctgc gggaaaacca cgctggcgcg cattctggcg acgcgcacat ccatgcattt
     6121 cgcagctctc tccgccgtct tctccggcat ggcggatttg agaaaagcct ttgacgaagc
     6181 ggccaggcgc agggaatacg gccatgggac cctcctgttc gtggatgaaa tacatcggtt
     6241 caaccgcgcc cagcaggacg gttttctgcc atatgtggaa aacggaaccg ttacgctggt
     6301 gggggccact acggaaaacc cctcctttga actgaacagc gccctcctct cccgctgcaa
     6361 agtctttgtc atgcactccc tggacgcccc ggctctggaa agcatcattg aacgggcgga
     6421 aacccttctt cagcgtgaac tgccgctcgc gccggaagcg cgcgccaccc tgatagaact
     6481 ggcagacgga gacggccgct acctcattaa cctgatggaa agcgtctttg acctctgcaa
     6541 gcccgatgaa atgctggaca cggacgcact gctgaaaatt gtccagcagc gagccccggt
     6601 ttacgacaag gaccgggaag gccattacaa tctcatcagc gccctgcata aatccctgcg
     6661 ggggtcggat acggacgcag ccctgtactg ggccgcacgc atgctcgagg gaggggagga
     6721 ccctctctac ctcctgcgcc gcctgacccg tttcgccatg gaggatatca gtctggcgga
     6781 ccctgccgca cttcaaatgg ccattgccgc gtgggacacc tatgaacgcc tgggctctcc
     6841 ggaaggagag ctggcgattg ctgaactgat tatctacctg ggctgcgccc ccaaatccaa
     6901 cagcgcctac accgcgtggg gagcggcacg gaaagcggca cgggaatacg ggtccctgat
     6961 gcccccggca cacatcctca acgcgcccac caaactaatg aaggaactgg gctatggaaa
     7021 gaattacgca tacgatcatg acgcgccgga cgctttctcc gggcaaaact actttccgga
     7081 caacatgccc cgccgccagt tctacaggcc gccggaacgg ggttttgaac gggaaatcaa
     7141 caaacgcctg gcctattggg acaaactgcg ccgccagcgg gcggaagagg acgccggaaa
     7201 ctccggtacg cgcagtaaca ggaaaaagcc tcctgttccg gaaaaacggg acccctcatg
     7261 acgcatccgc cggccatacg gccacttccg caattccctg ccccggagac aggcggatgc
     7321 gtacgtacct ggcgccggtc ccttccggac acattgcggt cagctccgtc ccatccttgc
     7381 gcaaaacggc tttannnnnn nnnngtccct gctgacctga accgagggag ccggcccctt
     7441 ccagcttccg gctgccttca cctccacccg gtccaggcgg aattcaaact ccatgtccag
     7501 ctgccaccac ggcgctttgt cttccacgga cggatgccat gcagaaacat cgtcaccgtc
     7561 ggaagccttc atcgcctcct gaaatccgga ggaagcggaa cagggtctca atacagccaa
     7621 attggctttc tctccggatt cgggccgtcc gtaccgcgcc agctggatat ccctctcctt
     7681 tgcgctaaaa cgcttgtagg gcctttcccg cgtttccgcg ctcctgcccg ctacgtaagc
     7741 cggagcattc cggcaaacga tttggaggct atcccccttc aatccgggag aagacgccct
     7801 gatcactgtc ttcccggcat aataagaccg gaactcaatc gccgcacagc cgtcaatcaa
     7861 atcaatatcc gttccgggcg tgaacgtaat gcttttgccg gtggggaact ccccgggacc
     7921 cgataccacc gtaagcgtca ccggcacggc attggatatc tgccttccgg cggcgtccgc
     7981 cactttcacg gtcacatgca catcgtccgt gccgtccgcc gggctgataa ccttcttgtc
     8041 cgcggacagt ttcacctgtg ccggagtccc ctctacaggc cactccggag gaggaatatt
     8101 cctcaacgca ttcctgtacc agtaccaggc ccgcttggga atgcggaagt aatccacaat
     8161 gcccatgcgc cctccggaag gccagatgct gccgtggtca aagccgcacc agaccgcctc
     8221 tcccacgcgc cagggatagc aaatccccgc ttccttatcc ttcttcatat ccccccagcc
     8281 cggcgcatac gcccccggcc tccggcagct cacggaacca tactccgtca ccatgctggg
     8341 cctgccgggc gtcttcacat gggaaccgtc cccgttaaag gcggccatgt cccccagctt
     8401 gtcaaaattt ccgcgctgcc cgcccccggc gcacacgggg cgcgtcgggt ccaactcctt
     8461 cactaaggta atgagcctgt tgcacaaagc cctggcctcc ggcgcatgcc tggtaaaaaa
     8521 cggttcattg ctgatgctcc agatgacgat ggaaggatgg ttgcggaatt gaagcaccat
     8581 ctccctcacc tggctcatgc agctttcctc aaacgcaata cggtccttct tttccgcagg
     8641 ataggcatcg caattccagt atctgtcatg ctccttgaac ccgcccattc cccagaaaat
     8701 gccttcattc agcatgcaca tgccctcccg gtcgcaagca tccagaaaag actgggaatg
     8761 agggtaatgg gaaccgcgga tgaaattaaa cccggcgttc ttcatcagcc gtacatcacg
     8821 gcgggccccg gcgtccgtca cggcatcccc ccacccggcg tggtcctggt gcacattggc
     8881 cccctgcaga taaacaggtt tcccgttaat cagcatgccc cggtccgccg tcagctccag
     8941 agtacggaaa cccaggggat tttcagcccg gtccagcacc ttccccttct cgtccaacaa
     9001 ttccgtcgtc acacggtaca tattgggggc cgcagggctc cacaacctgg gagaagccag
     9061 aggaggcagg tcagcctgta cgcgagaatc ttccccggcc tccagttccg cgcccttctc
     9121 tccccgcagc accacgcagc cgctttgctc atcccgcaca gtatgccgca ccgtcacttt
     9181 cctcactgtc ccggtatcat tcttcacttc cgtcaaaatc cggaccttcc cgcgggccgc
     9241 cgtcacctcc ggcgtctgaa cccatactcc gtgcgcgggc aaatacacgg gagaacacac
     9301 gtgcagatgc acgttccggt acagtcctcc ggaaaaagta tgctcccccg cccgaggcgc
     9361 aatgcggggg ctccagcggt tatccacccg tacctccacc cagttctcgc cgggcttcag
     9421 gaaaggcgtc aaatccgacc ggaaggccgt atacccccct tcatgccttc ccgcagtctt
     9481 cccattcacc ttcacctccg ccacctggaa agctgcgccg caatccagaa acaccttctt
     9541 cccctgccat tcctccggca tgacaagctt cttgcggtag gagccgaacc ccgtgtaaaa
     9601 ggaatcggac ataaagtagg gcagactgaa cgtatggggc agatgaaccg tctgccagcc
     9661 gctctttccg ggctcgtcct caagccggaa ctcccagccg cgattccaga tcatcctccc
     9721 gaacaggggt gaagcttttt ccgcagagga aacgcctgtt tccgctccgg ccatggaaaa
     9781 acacagggca gcgcccagca tgcaggccat catcttgcgc atgcgtatgt taccattccg
     9841 ccatgttctt tctttcatta aaaaaacatt tccgcaccct gaaaagcccg caacaacaag
     9901 ccgtgcaccc cggaaagggc gcacggctgg aaaacttctc catccgggaa gcagtgcaaa
     9961 acgcctgccg ctccgttatt gggtaatgat attcagctcc gcaacggtgg cactctgttc
    10021 cccgcccctc accggagacc gggcgtcaag cttaaagtag cgggcctcca caggcctgcc
    10081 gaagtcgatg cgctgaacag ccggatcctt ccggatatag tggtaggaga actggccttt
    10141 tttcacttcc ctccaattcc gaccgtcatc ggaaacggaa aacgtgtact ggctgatgag
    10201 gcccttgtcc ttgtccattc tgggcgtgta gataaatccg gtaaacttca ttctggttcc
    10261 catatccacg gcaatggagt gggggaaccc cggcaggccg ttcgtatagg acgtatgcca
    10321 ataggtatcc ggattcccgt caatggcaaa atgggcgaaa ccggtatccg gctcttccga
    10381 agaagcgctt agaattttcc attcgccctg ccccttgacg agggggaatt tcctctctcc
    10441 gggagacgtg gaaggaacct tgccggactt catggaggca gcccaggccc tcacttcccc
    10501 ttcgggaagt ttgaagggat tccggtacgt tttttccggc cccttgttga cggaatactt
    10561 tatctccgcc tcgggcgtag aggaagtcag actgaccatg ccgtccccat cccgttcaat
    10621 gacaggggaa tggggaacgg ccaaccccgt gcgcgccaga ttcagcaggg cggatgaggg
    10681 agccagcaca aaccccaggg ctgtggcccc gctcaatgtc tggtacttgg ccagcggacg
    10741 gggcccgcag gaagagccgc ccagccccat ctggaaagca tccagattca caatcacttt
    10801 atttttctcc ggcagcctgt ccaggctctt ggcatcgtta agctccttcg gcgtataacg
    10861 caggacggaa acctccagcg gagccctggg agaggcggcc cccacccaca gagcctggtc
    10921 ctcctggggt ttggacaagg ctatccagtc cgtttccatg cggttgccca tatcctgggg
    10981 ccgggaataa gcaaaaaaca tatcgtccac ggaagtgcgg aaaatgtcct tccagcagct
    11041 cgtgcggcgg tcccggtaat tatcccacgg tcccagcccc aggtactcta cccggtcata
    11101 cgcggcaggc atcccgaaag tgacgcccag ccggaggagt tcttctccct tagcggaggg
    11161 atacattcgg acggaagcat tgataatgcc gttgccgaaa accgtccacg cgacttcccc
    11221 ggaaaaggca agctcggaac ctttcgtccg gaactgtgcc gtgattcggg ccacgccggg
    11281 ggaaatgacg gcagctttaa cgtcggaata ttccgctttc atattctgca tcttcatttt
    11341 cccttcaacc ttgctcctga tccaggcatc gttatccacc ggagaacggt acgcctggag
    11401 ctccaccgga tagccgggca aaatcaaatc ccggtcgcca taccggaggg aggaaagcat
    11461 cccgtttttg aaggaggcgg aaaaaccttt tccctgcacg gccacggaat ccccctggag
    11521 cacggaaaca gcaggcagcg ccgccggggg aggcacggga acctccttct ctgcagatgc
    11581 cgggaaccac tcgtaagctt cgcactcatt caccacatgt tccagttgct tggaggaaag
    11641 ttccttcacg tcttccggga gcttgaaccg ggcatccgcc agaaccatca gggaaccggt
    11701 ccgcatagcg gctgaaggca gattaaccgt tgcggatttg ccgggaggaa cctccggcag
    11761 aaccatgcgc tccacggcat ggccgccgcc gggaacaagg gataccagat aaagcgtata
    11821 acctttcaaa ggcttatgga aatatttgtt gcgaacggac agggacccgt ccttgcgctc
    11881 aaaagccatg tactggtata ctttcttcac ctctttcgtc ttggccgtgg tgttccggtt
    11941 gccaagaata atgccgttgt cgcagaaatt ggcctggttg ggcctgtctc cgaacatgcc
    12001 gccgtaagcc tgtgttactc ccttgaaagg ggcgggcttg taaatgccgt ttccggcagg
    12061 attggcgcgg agggactggt ccacgaaatc ccagatggcc ccgcccacca tgcgcgggga
    12121 agactcaaag gcatccatgt actccttgaa attccccagg gcattcccca tggagtgggc
    12181 atattcgcac acgaaaaagg ggcgggaagt tttctgcttc ccccagtttt ccacccggtc
    12241 cacgggcgga tacatggcgg aatccatatc cacggccttg tggccgtgcg gaaattcgca
    12301 gtaatggagg ggccgtgtag tgtcataagc cctgatagcc gccgctgccg cctcaaaatt
    12361 ggggccgttg ccggactcat tgcccaggga ccagaaaaca atggaagggt ggttcttgga
    12421 acgatgcacc atgctcatat tgcgctccac atgggctttc ttccaggaag gcttccggga
    12481 aatatccatg ctgctattgc ggatgccgtg ggcttcgcag ttggcttcgt ccatcacgta
    12541 caggccgtag cggtcgcaca actcataaaa ataaggatga ttgggatagt gggaacaacg
    12601 gacggtattg atattatgcg ccttgataat ctgcacgtcc ttttccatca cttcccgcgg
    12661 aatggcgcgc ccgtaatcgg gatgggcttc atggcggttg acacccttga acttgacggc
    12721 tctgccgtta atcagcacgg cgccgttatc cgcaatctcc acggaacgga acccgatatt
    12781 gacggaacgg gtatcctttc cattcagaga ataagtcacc ctgtacaggt tgggcgtttc
    12841 cgccgtccat ggcttcacac cgggaaattt cctgttcagg gaaatctgca cctcgcctcc
    12901 ggagggaatg gagggaacct ccagcgttcc ggaatccagc cttcccctgt tatccgtctg
    12961 cacgcggccg gaaccgttcc tgccctccca gttcaatttg gtggaaatcc cgtcgatgga
    13021 ataatccagc ttcatgccgg cgggaatatc ctttttaccg gaattgcgga tggtgaacgt
    13081 tccgtccagt accccggttg tgtaatcctc cctgtccagt ccggccttca ggaacaggtc
    13141 acgcacgtgc gtctgcggct gggcaaacag ggtcacgtcc cggaaaatac cggaaaggcg
    13201 caggaaatcc tggtcctcca ggtaagaacc gtcgctccag cggtatactt ccacggcaat
    13261 cgtattcctg ccgggcctga catagggagt aaggtcaaac tccgcccctg tataggaatc
    13321 ttcggaatag ccgacttgac ggccatttac ccacacataa aaggcggact ccaccccgtc
    13381 aaaccggatc atcacctgct ctcccttcca ggaaacgggc aaattgaaat cccggcggta
    13441 ggaacccacg gaattacgct ccctgtacgc cggccagttc ttgggcggtt cccccattac
    13501 cttgggagga ttcggcttga atggataggg aacattcgtg taaatggccg ttccataacc
    13561 gtaaagctgc cagttggaag gaaccttgac cgtatcccat ccggaaacgg aataagaggg
    13621 attgaagaaa tcggcaggcc tctcgtccgg agtcatggaa aatttgaact tccaatcccc
    13681 gttcaagctc aacgttttat ctgatgccgg aaaaacggtg gctcggggag cctgcttagt
    13741 gattcccgtc accgcggcat tttcccaatc cggaacatcc cccagcaggg gaagagccgg
    13801 agccagacac atcacggaaa acagaaacgc gcgtaccgtc ttcatctcgg aatgaatatg
    13861 gaattgctta cttatacttt ttcagggacg gatcctgata acgctctgaa tccttcttcc
    13921 cgtcacctcc ggcagggttc aacgtccact tgcaggaatc cgtgtacgtt ttccctccta
    13981 tggaagccgt ggccgtaata gtgttgacgc cgggcttgag agtcacttcc ggccaaacgg
    14041 cgcgcttcaa atcatccggt ttgacactcc caacgggctt cccgttgacg ctcagcttca
    14101 ccacggaacc attgctgtaa accttcacgg gcaccgtttt ttccgtacgt tccgcaaacc
    14161 tgcgggatgt gatgtatacg gtaggacggg ggctccagtt ggccctgtag aaaaaatagg
    14221 cgtccttggg tgtcttgcgg tcataggtca ccagcccttt gtcgttaatg ccggggcttt
    14281 ccccctcaaa acgggaagag gaaccgaagt caaacatgtt ccagacaaag gccccccaca
    14341 cctccggcct ttccctgatg cacctgtaat tgatctcatg cgcatgggac tgccattcct
    14401 ccggatgcca gaatccggtg ggagacggcc ttttggccgg attctcatgc atgcccgcgc
    14461 tggcgccgtg accatactca ctgacggcaa tgcccatctt gccgccatag tcgcggatgt
    14521 agccgttcag gttacccttc atggtttcgg gccctcctcc gtaccagccg ggatacgtat
    14581 tgaatgcgat gtggtcagga atattgttca gttttctgcg gcccgcctga ttgctggccc
    14641 ccacggtcat gcgggaggga tccgtctcct tcacgaactt gttcaattcc gtcaactcag
    14701 cttccatgtc cactcctcta gtggcagcct cgggctggtg tccgatttca ttgaaaattc
    14761 cccacatgca aatggaggga tgattccaat gctggaaaat catctcccgg gcctggaggc
    14821 ggttattctc ccggaactcc ggcgtatccc acaccaggtt gacattgggg acctcggacc
    14881 agacaatcag ccccgccttg tcgcacaggt cgtaaatatt ggtggatgcg ggatagtggc
    14941 ccgttctaac cccatccgcc cccatatccg caatcagcct gatatcccgc gcctcatctt
    15001 ccggtgaaag ggcccacccc ttccccttca tatcctgatg gcggctgacc cccttgatct
    15061 gcatggtctt tccgttcagg acaaaacctt ccttgggatt gacggaaacc gtcctcaggc
    15121 ccagcggctg caccagctca tccatctggc catccgccgt cctgatggaa gttttcacct
    15181 ggtacaggta aggattcttc actccgttcc agagtacggg attggcgatt gccagcgtta
    15241 cgggaacctc cagtctattc ttttccccgg cggcggcctt taccgtcttc ccggctacct
    15301 tcctgccctt catgtccagg atttccacgg caacatccac atccccggcc tttccgtccg
    15361 aattgagaag ggtcttcact tccacctcgg ccttggattt gcttaaagac tttgtggtga
    15421 tgaaaacgcc gggagaagca taaaaaagag gatcaatgca gacaccgtcc gtctcaatca
    15481 gttcaatggg ccggtacagc ccgccgaaca tggaaaaatc cccgcgctgg ggggcgatat
    15541 gattacgctg cgtgttgtcc accagaacgg aaatcgtatt ggagcctttt ttcaaaaggg
    15601 gcgttatttc ataacagaaa gccgtaaacg ccccctcatg gctgccggca ggcttcccat
    15661 tgacggaaac ctcggaaaca atgctggctc ccccgccgcg gatgaaatac ctcttgcccg
    15721 gcctgggctc caccggaagc gtgcgcgaat aaacggccgg ccctcggcgg tagnnnnnnn
    15781 nnntgatttg gcgtcttccc gcttcccctg agcaccattg gcggcatcct ctgcgttcca
    15841 tgtatggggc accgtcactt tttcagcagg cttcccgtta aaactgaaat cccaggaacc
    15901 gttcagggaa taaatctcac gggcgtccgc cgcgaaaaaa gaccccaata cgcagcaaag
    15961 gccggcacat gatacgttga aagaatgaat tctgatgttc atgatacagt gaaatagtat
    16021 atcccatacg tccggccatg taaaatactt tcatccggcc gttcctttac ccctcttcca
    16081 acagaaatat actcatgccg gacaagtcac gtccatcttg acattcagca cagggagccg
    16141 cgctttctaa cattttcggt tcctgcacca tcagaaactt tgattttccg gccggttcaa
    16201 agaaaacata agtaacaaca tatccttgac ggttacgcgt tttctagtgg aaagcataag
    16261 ttttccccat tataaggata gcctttttca ttaaaaccgt gtttggacaa acacgagaaa
    16321 tgaacatgaa aaaagcaccg aatacaaaca aattgaaata tgtcggagcc gcacttctgg
    16381 agggggaatc agtcaccctg atccaggctg caaggctggt attagaaatc aaggaagccc
    16441 ttggagatga aatctgtacc attacccggt gccgggaagt cgtctccctg ggattgaacg
    16501 ccattaaaaa caaacatcaa accgtcagtt tcggcacggc ggccgtggaa tgcctgcgtt
    16561 ccaaaagcca ccgccgtccc cggacgctga cggacatcag gagcatcatc cacaagctta
    16621 aaaagagcaa tccggaactg gaacacacct ccctgcgcaa cctgagcgtg gaggaatgcc
    16681 agaacatcct gatgaatacc tttaccacat cccggcagag gcacaaagcg aggctcatct
    16741 tgagcggaat tttctcgttc tccgtcaaac gcggatggtg tgacgagaat cccatcctcc
    16801 gcgtagacac gccttttctg caggagcagg aaatccccgc tctgacgctg aaggaaataa
    16861 ctcagcttct caaggcggcc atggaagaat ttgacggaag ctgcgcagct ggagcggcgc
    16921 tgatgatctt tgcaggaatc cgcccgcagg aagtggaacg cctgctctgg gaaaacatcg
    16981 cnnnnnnnnn nccgcgacgg ctgtatcatt ctgaactcca agcataccaa aaccggaggc
    17041 gccaggcacg tcaccatcct gcccgtgctc gccaaatggc tcaaattctg ccgtgacagg
    17101 accaaacccg gccccggaac tcccatctgc ccgaaagggt ggacaatcaa gtggcgcaaa
    17161 atccggaaaa aagccggctg gggaggaaga aaaaaatcat gggtgccgga ctgcctgcgg
    17221 cacacctacg ccagctacca cgccaagcac ttcaaggact acaacctgtt gcaaatggaa
    17281 atggggcacc gctcctcctc cctgctccgc acacggtact tgaacatgaa aggcatctct
    17341 ccgcaaacgg ccacgcgctt ctgggccctg acgccagcca aggtcattga agaaacgaaa
    17401 ctgccggaag aaccgccggt ctcctgatcc gcaacaaagg aaaacgcgcc ggcacgggac
    17461 aaacgctccc tgttgaagca aaagacaaga ccgtttccta cagttcgtaa taagtgtatc
    17521 cattcaatcc ggaacggaac acttccaagg ctctccggcg ttcggacgga gatattttcc
    17581 cctcggctac ggcttgctcc gcaaacgtgc ggaacttgtt gatcaactcc ttgggatcat
    17641 actccacgta ggaaaggaca tcggacaccg tatcgccctc cacttcatgg gtataaacgg
    17701 ggcggccgcc ttccagatgc acgccaacca cattcgtatc tcccagcagg ttatgcaaat
    17761 cccccaaggt ctcctgatag gctcctacca ggaatacgcc tatgtaataa tcgtcctcgc
    17821 cgggtttgat ggcatgcagg gggagggcgg ttgccacgtc ctcacggtca atgaaatggt
    17881 ctatctttcc gtcgcaatcg cacgtgatgt ctgccagcac ggccttattc gtgggccgtt
    17941 cattcaaacg gtgcagaggc atcacgggga aaagctggcg gatggcccag gaatccggaa
    18001 gagactggaa cagtgaaaaa ttgccgtaat aaaagtccac catggagcag gaaagctccc
    18061 tgagatcctc cggaatggtc tccatctccg ccagcatgcg ggaaatgcgg ctcaggatat
    18121 gccaataata agcctctccc agcccgcgct gccgcagggt ggcgttcccg taaaaaaact
    18181 gggctctcaa ctgatcccgg taataacagg cgtcattata gcactcctgc atattcttcc
    18241 tggccagcgt ccggtccgtg tccgcaaaag ccttcaacag ctccggagcg tcatccggaa
    18301 gctcgggcgg aggttcctcg cccggagcgc tcgtcacatc cagcacgttg aacaccagca
    18361 cggaataata ggccactacg gcccgtccgg actccgtcac cagcgtggga tggggaaccc
    18421 cgtacttgga acacatgtct cccacaacct ccaccacgtc acgggcaaac tccgcaatgg
    18481 aataattgca ggaagaatgg aaattcgtct tggaaccgtc ataatccacg gccaggccgc
    18541 cgcccatgtc cagcgtgccc agaggggcgc cctccttcac caggtccacg taaatgcgga
    18601 ccgcctccgt cagcccctcg cgcaccacgg aaatatttgg aatctgggac ccctgatgat
    18661 aatggagcag cttcaggcag tccagcgcat ccacctgctt cagcttatcc accacatcca
    18721 ccacctgggc tgcgttcagc ccgaaaacgg acttatcccc cgcggattcc tgccataacc
    18781 cggacccctt ggccgccagc ttgacgcgca cgcccaaatt agggcggata tccatccttc
    18841 tggcgcgctc cagaataacg tccagctcgg aaggcatttc cagcacgata aaaatattca
    18901 gccccatctt ctgggccgtc aacgccaggt caataaactc gtcatccttg tacccgttgc
    18961 aaatcagata ggcattcgga ttgtgcatat gggccatggc ggctatcagc tccggcttgg
    19021 aaccggcctc cagaccataa tcatacttgg tgccgaactc cgcaatctcc tccaccacct
    19081 ggcgctgctg gttcaccttg atggggtaaa cgccctggta cttaccctgg taccccgcct
    19141 ccttgattgc tttccggaaa ctgtcgttca gctccgcaat acggctgcgc agcaaatccc
    19201 ggaaccggaa cagaacggga acggtagtcc cgcgctcctt cagcccctcc agcaaatcgc
    19261 acaggctgac atccttcttt cctccatctt cgtcattcag gcgtacggta acctctccct
    19321 ttttgctgat gcggaaataa ccgttccccc aatcgtcaat gccgtacaaa tctgcggaat
    19381 catggtgact ccacccgcgg actttctgct tgatgccttc tttagctgct gccatgtcaa
    19441 tctgtctggt caaatattct agcccaggcg ggcctcaaaa tcttcatagg aaaaacggcg
    19501 caccgtctcc acggaaccgt cttttctctg aacggcgatg tccggatggc gcacgccatt
    19561 gaaaaacgta gtcttcacca tcgtataatg cgccatatcg tcaaacacaa gttcgtcacc
    19621 taccgccagg ggctccgcga aggaataatc tccaagccgg tctcccgcca ggcacgtggg
    19681 ggcgcccagc cnnnnnnnnn nccccttcca accgtacggc aggctgctgc gcggaacgga
    19741 gggaagcgtc tcctgcaatc tggaacacat ccggccgata aggcatttcc agcgtatccg
    19801 gcatgtgggc ggatgcggaa acatccagaa tggcatgatt caccccctcc gcggaaaaaa
    19861 ggtccagcac ccggcaccgc agcaccccgg tatgaatggc agcggcctct cccggttcca
    19921 gccatacttc cacgccgtag cgttcgcgga tgcggcggat aagtgccgcc agcagcttcc
    19981 ggtcatagtc gggtttggta atccaatgac ctcctcccat attcagccag gaaatctccg
    20041 ggcggctcaa aagatggccg aagcggcgtt ccaccgcatc catcgttacc gccaggtcat
    20101 ccgctccctg ttcgcataaa gtatggaaat gaagaccgga aatgccggac aggtccgccc
    20161 cctgcatcat gtccggcgtc acgcccaggc gggaaccggg ggcacagggg tcatatatgg
    20221 gcgtatgccc ggtggaacat tggggattga cgcgcaagcc gcatttcaat tcaccggaaa
    20281 caaagcgggg atgccgcatc acttcctccc ggaaacggcg ccactgcgtc aggctgttaa
    20341 aatcaagatg gctggaaatc tccgccagct ccgctatttc ctccggggca tggccgggag
    20401 aataagtcag cacatgcttc ccgaacctgc gccgggccag ccgcgcctcc cacaggccag
    20461 aagcgcagca gccgtccagc cagggggaca gggcctcaaa acagggccag caggaaaacc
    20521 ccttcagcgc cagagcgatg cggcaccctg cggcatccgc cacatcccnn nnnnnnnnca
    20581 tccctcagga taagagcgtt gcgttccagg gccggcaggc tgatgataaa ggcggacata
    20641 aagcaacgac aacctaagcg attcagaacg atttgcgaga aaaattttag taatttacca
    20701 tcatacctgc gagacgggca tgagaacgcc ttccccactc ccgccagccc cccgcatcaa
    20761 acggaaacgt caaaaaacgg cacaaaacgg tacctcctgc attcttttcc ttgaaatggg
    20821 atgtattttc ctcttttata acgtaaccat tgcgattgcc atgactaaca ttcatccctt
    20881 cactactgca gcagcctgca tcctgaccgc cggagcagcg ctcttcacca gctcttgcca
    20941 acctgcctct cccgagagct atgcccgtca atttgctact gcccagcacg tatccgtgca
    21001 cggctacaat ggccgtgaaa cgctgacctc ccgggtttac gctgactggg gcagcatgcc
    21061 ggccaatgcg cgccgcgcca tggaggaata catcaagggc gctgaatgga aaacgatgga
    21121 ctacatccgc ccgcagtact tcatccaggt agaccgctct ttctgggcga tctgcattga
    21181 caatgacggc aacatgacag gcatcattcc cttccgcaat ggcaaggacg cccgcaagat
    21241 gtccgtcaac aaccacttca agatgctggt caacacgacg gaccgggctc ccgccctagg
    21301 ctatgccatc ctgaaaaacc tggcttatgc tgatggactc cgcgtcagca cccgcaaggc
    21361 tgacgggctg gaaactcccg ttcccgcccc tccggccatg aaagtcgttg ttccgcccaa
    21421 gggagctgct ccgtccgcag cgcccgctgc caaatccgca gcaacgtccg tttctgaacc
    21481 aggcgctacg gaagaaactc ctgccgacga tactgccgga acggaagagg agccctctgc
    21541 ggacagcgct gctgaaagcg aagataccac gacaacggac gatgccgctg tcaccgacga
    21601 tttttaaacc ctccctttca ggggactttg ttatttaacc cttgtcacgc cccgacgcca
    21661 tatccgtcgg ggcgtttttt atccctcccg aaccagttcc cttgctcatt cctccccttg
    21721 ctccgaaaaa agctgaggcc ttcattcacg aaaaacgatg atcctgcatc cggaaaagaa
    21781 aacacacacc attcctatac cggacagaaa aaactactgg aaaaagaagt gagggcaccc
    21841 cagcctcgac ccggtccgcc cggcgggatg tgaatcctgc cgggggttat gaaaaaactt
    21901 tggaaaaacg ttattgcgga tcatcaaaaa ccattcccct tcacctggtc gggccacgga
    21961 acggaactcc cggcaggaac gcccagctca ttgtccttct tataggttgg catctgcgcc
    22021 ttcctctccc ggagcaacct gcccatgaca acagccattt ccctggtttt ccgcggctcc
    22081 ctggaagcca ggttttcctt ctcgccgata tctttcctta aatggtacag ctcaaagctc
    22141 tgggtcaggt ggcggtaaat caatttccaa tccccctggc gcaatgcggt ggaagaagta
    22201 tactgaggcg cctgccggct gccttccccc caattattcg gctggtggaa caaaaggggg
    22261 cggtcttcag gaaaccggcc gccttcgagc aaaggaacca ggcttaagcc gtcaagcttc
    22321 tgcggaactt ctacgttccg gcatccggcg atatccagaa gagttgggaa aaaatcgtca
    22381 ataaccaccg ggacggcaca ccttgaaccg cccttcgtca cgcccggcca ggaaacaatc
    22441 ataggctccc ggataccgcc ttccatgcat gaccccttgc cgaaggaaag agggtaattg
    22501 gcctcttcat tgcccagtct gccggagatg gccaggcctc cgttatccga cataaacacc
    22561 agtacggtat ttttttccag atgatgttcc ctgagatact tcatgatatc ccccaggctt
    22621 ttgtccatcc cctcgatcag cccggaataa tgtttctccg tccgggacca cttgtggccg
    22681 tcctccgggt ttttgtaggc atccgcaaac ctcttgtcgt aagcgcgctc atccagcgga
    22741 gaatgcaaag cgtaatgggc catgtacaga taaaagggct tgccagcctc cctgggattg
    22801 gtcctgatgg cttccaggcg tttaatggcc tcccgcgtca gggcctccgt caaaaataca
    22861 tcgtcctggt aataattatt ctcatccagt ccgcgcacat ggttaaaccc ctttccgtaa
    22921 tgcctggaac cgcggtaatc cgccggatgg ccgatctccg tacctgcgat attataatca
    22981 aatcccatat tcaacggatt ggaacccgga gttccctgcg tgccgaaatg ggccttcccg
    23041 caatggacgg taacataacc gcacttcttc agcatctccg gcagtgtagc cgccgtggca
    23101 aaaggacgcg tcatgtgata gcgcatatcc tccccggaaa tggggcgttt tgtcgttccc
    23161 ctggcgggag tgcctatagg ctgaaggccg ttcaacgccc agtccggagg caggaggctc
    23221 ctgctgttgg cgtccgtcgt cccgttctgt tcccgtaccc agcaggtcac gcggtgccgc
    23281 gccggattca tgccggacat caggctcacc cgggaaggag tgcaaagggg atgggcatat
    23341 gcatccgtaa acgtcatgcc ctgggaagcc agcttctcca tattcggcgt ccggtagcgc
    23401 ctgttcaaaa aggttttctt cgggttgccc tggctgtcgg accagaacgg gtgggaagta
    23461 tcctgccatc ccatgtcatc taccaggaaa acgatgatat tgggcttctt tccggaaaca
    23521 gggttggaac cattgcccag agcggatgcc ccggcaaaca ggaacaatgc tccgcagacg
    23581 gcggacataa tacaggaagt aaacttcatg gtcgcaatca acaaaaacct ttcagtcaag
    23641 gaatacgcgc aaacgtcttc ttacctttcc ccttttttcc gaaaaacggc atcatgcccc
    23701 cggagcattg gaagcgcctg cattccgccg ggtttaggct tgattgcccc gcgggcggag
    23761 tatacattcc ctcccgtcat gtccgagcgc aagaaaccat atccctttga cgtttttgaa
    23821 cctaaatggc aacagatctg ggacgagagg aaaaccttta aagtaaacaa ccccggagaa
    23881 gaaggctttg atgcgtccaa acccaaatac tacgtgctgg acatgttccc ctaccccagc
    23941 ggggcgggcc tgcatgtggg tcatccggaa gggtacaccg ccacggatat tgtagcgcgt
    24001 ttcaaacgca tgaacggctt caacgtgctc catcccatgg ggtgggactc cttcggcctt
    24061 ccggcggagc aatacgccat taaaaccgga cagcacccct ccgtcactac cttccggaat
    24121 atagacaact tccgccgcca gctcaaaatg ctggggttct cttacgactg ggaccgggaa
    24181 atagccacaa cggaccatga atacgtccgt tggacccagt ggatattcct ccagctttac
    24241 aactcctact acaacaagga actgaaaaag gcgcgccccg tttccgaact ggaggaacag
    24301 ggattgagcc gggaagaaat cgaccagcgc cggctggcct acgtagcgga agctgccgtc
    24361 aactggtccc cggacctggg gaccgtcctt gccaatgagg aagtggagga atggaaatcc
    24421 aagggacacc gcgtggaacg ccgccccctg cgccagtgga tgctgcgcat cacggactac
    24481 gcggaacgcc tcattgacga gctggagccc ctggactggc cggaatccat taaactgctt
    24541 cagcgcaact ggataggcaa atcggaaggt gcggaagtgg acttcacctt ggacggagaa
    24601 acgatcactg tttacaccac caggccggac accctctttg gagccaccta catggttctt
    24661 tccccggagc accctctggt agataccgtc accacgccgg aacagaaaca tgctgtggaa
    24721 caataccgcg cccagtgcgc ctccaaaagc gacctggaac gcacggacct ctccaaggaa
    24781 aaaacagggg tcttcaccgg agcctatgcc gtcaatccgg tcaatggaaa acaaattccc
    24841 gtctggatcg cggactatgt gctgatgggc tacggaaccg gagccatcat ggccgtcccc
    24901 gcccatgacg aacgcgactt cgcctttgcc caggtcttcg gcctgcccat tctccaggta
    24961 gttcagccgc ccagtgaaga catggactgg cgcggcttct gcggttatga aggttccagc
    25021 gtgaactccg gcttcctgac cgggctgcct actccggaag ccaaggaaaa aatgatcctc
    25081 tggctggaag aaaacggcaa gggacgccgc aaagtaaact acaagctgcg cgactggctc
    25141 ttctcccgcc agcgttactg gggggaacca ttccccatcg tatgggaaaa cggtcgccac
    25201 cgcgccctgc cggaaagcga actccccgta ctccagccgg atctggacga cttcgccccc
    25261 acgggagacc cccgcggccc gctggtcaag gctgcggaat ggatagccta cacgcccaca
    25321 gcccaccggg aaacaaacac catgccccaa tgggccggct cctgctggta ctacctgcgt
    25381 tacctggatc cggccaacac ggaacgcttc gtcagccggg aagcggaaca atactggatg
    25441 ggctccgccg gctcccccgg aggcgtggac ctgtacgtgg gcggcacgga acacgccgtg
    25501 ctccaccttc tctatgcccg cttctggcac aaggtgctct ttgacctagg ctacctcagc
    25561 accaatgaac ccttccagaa actcgtcaac caggggctca tcctggggga agacggccag
    25621 aaaatgtcca aatcccgcgg caacgtcgtc aatccggacg acatcgtccg cgaatatggc
    25681 gcggactccc tgcggttata tgaaatgttc atgggccctc tcaaggacgt gaaaccctgg
    25741 gcaaccaaag gcgtggaagg tatttcccgc ttcctggccc gcgtctggcg cgtggccttc
    25801 cgggaaaatc aggaaggaga atgggaaatc aactccaaac tggtggagaa cgctccggaa
    25861 gccggggtgc ttgccgtacg caaggaattg cacaaaacca ttaaaaaagt gacggaggac
    25921 atcaatggca tgtccttcaa caccgccatc gcgaaaatga tggaatgcac caatgccatg
    25981 acctccgcgg atgtggtgga cgttcaggac tatgatgctt tcctgactct gctgaatccc
    26041 tttgcccccc accttacgga agaaatacac agccgcctgc aaacagcctt cccggctctg
    26101 gcccagacgc agctttgcca gaaaagctgg cctgaatggg aggaagccct gctggaggaa
    26161 aataccgttc ccatggtcat ccaggtgaac ggcaagctga gggacaaact ggaagtgccc
    26221 aaggacatct cccgggaaga actggaaaaa caggccctgg cttccgccaa ggtgaaaacc
    26281 ttcctggacg gcgtcactgt gcgcaaggta attgtcgtgc ccggccgcct cgtcaacatt
    26341 gtagccaact gacatcccga aaagcctccc tcagccatga ccataatctc cccgtccctg
    26401 ctggccgctg atttctcccg cattggggag gaagcgcggc gggcctttga cgccggtgcc
    26461 gactggctgc atctggacat catggacggc cactttgtgg acaacatctc ctttggcccg
    26521 gaaatctgcg ccgccgtcag aaaagcggtg gggccggacc gctttctgga tgcccacctg
    26581 atgatagaac ggccggacca ctactttgac cgcttcgtca gggcaggtgt gaacctcatc
    26641 tgctttcacg tggaactggg agactcctac tacattcgca acacactcgg gcgaatccgg
    26701 gaagccggag taaaaaacgg cctggctctc aacccggcca caccctttga aaccgtagaa
    26761 ccttacctgg cggaaataga cctgctgctg gtcatgtccg tagtgcctgg cttcggcggc
    26821 cagtccttca tgccttccgt attggacaag gtggaaaaag ccgccgaatg gcgcacggaa
    26881 aaccgcgcct ccttcctcat tcaaatggac ggaggaattg gccaaaacaa tgccgctcag
    26941 tgtgctctgg ccggagcgga tgtgctggtg gcaggctcct ctaccttcaa ggctccggat
    27001 atggcccgcg ccattgcgga aatgaaatga cccgcctggc acttcggaaa atcaaaaaat
    27061 ccggaaatcc gccctgaaac gccggagtcc atccccgggc gttccatcat caggctttag
    27121 ggaagagccc tactcaaaac attgcgggac aggcacgcca gcccggaggc tatgtcgctg
    27181 ccgtgcccat catgtctccc tgccgggcaa acagctccag gccttcttcc gtcttttcca
    27241 acaaatcgga agccaaactg actcttcccg gttcaaaaaa gcacattacc gtggagcctc
    27301 caaaggcaaa ataccccttc tccgcccctc ttgcggagaa aacacccgga acatacgtct
    27361 cttcaatcaa cccaactccg gtagctccca cctccagcat ggcgacctct ccccacaact
    27421 ctgaccggat cagggtcaaa ttccttttat tagtccatag ccacgccaga cggccgcgca
    27481 ggcaatacgg agatacgctt gccagcgggc ctcccagctt cgtccagggg ccgggcaccc
    27541 cggacaccgg aaaatgaaaa cggtggtaat ccgtagggca caggcgcgat aagacgacgg
    27601 cgccggccgc ataccgctca gccagggtgt cgctccccag aagggaaggc aggtcaaaac
    27661 gctgcccttt cacgaaaacg tttttaattc ggtcggcgcg ttcccaaccc atgtgccggg
    27721 catccgcagg aaaaacggcc gtatcctccc cgccggccaa agggcgagct cccggtttca
    27781 ggcggcggta gaaaaaatcg ttgaaatgcc tgaactcccc cactcccttg agagaatctt
    27841 ccatattaat gccgtactcc tccacaaagg aaggaatgga gcgggcgctg gaagggcgat
    27901 tcttcaacca acccagcaaa cgggaaaaaa cgccgcgttt aatcaggaca tgcagtgccg
    27961 ccttccccga agcagtgccg taaatccatt gcatatactt ctcccccggt acctgctccc
    28021 gttcctccct gcccgtatag cggttgtaat agcgtatgcc ctccatggaa cgcctagaat
    28081 gtcttccgtc cctccgtttg tccagccgaa agaatatccc tgcctcccgt ttccagcatg
    28141 cagcatgggg gcttcgctcc tcctcaccgt ttcccatccc tctgtccaag gcaccctgtc
    28201 ccgcccagga ttcaaatact gcgaagcatg aggcatcctg tctttctgcc gggccactca
    28261 gaccttgctt ttcaaaaatg gtaaacgaga aatacccacg ccctaagaac actctccacg
    28321 gccattcctt ctattccagc ctcctgtcta gatatcccat caaaactcct gaactatgaa
    28381 gcgcctctat ttctcctctt ccatcaccct ttgatactcc cgccacaact catcaatatg
    28441 atactcgctg cccaaagcat atttaacggc gccatcaaac gaccatggaa cgacctcaag
    28501 agcactacgg ttcaatttgc gtagaaattc agggtcttta taatcccgca gccacacaaa
    28561 aaaataacct gcatggaggt agccatccat ataactgccg cccgtaggac ggtaagctcc
    28621 tctatggaat cccccgttag ctatacgaac ggcgtcagcc atcccctcga taaatgctct
    28681 aaattccctg ttcgttccat aggaacctat cccctggggt tcctgttggt aaacatgggt
    28741 tagctcatgc aacaagactc ctcgaatttc aaaataaacc ttcgccgcat catcatcttt
    28801 aaacaacccc tcaatatggc gcgtgctgca ggaaatggca acctctccat ttcctcccca
    28861 tttgcaggca acgccatcgg tatcctccag catgaaacgc aaatggtgga caggaacaat
    28921 acattcttca ggactctcat acaacgtatg gagaacaata cgggacaact gatggatata
    28981 aacctccgta tctggaatca atcggtgaaa aatgcgggaa cctttactat gcggagcctt
    29041 atcatcgaat gaaacgcggc ccaaagaaaa attcctccag attatatccg aatcctcatc
    29101 cagaacaatg gctgaggcat ctttttccgt cttatgctcc ggagcttccg gaatacatga
    29161 aaatatcccc atgaacaagc acaaacataa cccggaaacc agacaatatc ttttcatatt
    29221 cgtccaataa tacttcccgt catttctcct ctatcagcca aaaaccaact tcctccgtca
    29281 aaatattgca ttcaacaaaa aacacaaaaa tccatctttc attcttcagc aacaaatacc
    29341 cttatttcaa tatatctccc tcccttaata aaacaacgat tcgtatttaa atatgtcaaa
    29401 catacataaa aacttcaatt cacgcgcccg tgtgggtgcg actcccgtcg tagttgacag
    29461 accgggaaat caacgtgttt caattcacgc acccgtgagg gtgcgacgga tcttgcgaaa
    29521 catcactcaa aaggatgaga agtttcaatt cacgcacccg tgagggtgcg acttctacct
    29581 cctgcggctg cgggattccg gaatctggtt tcaattcacg cacccgtgag ggtgcgacgc
    29641 gttgcccccg cagggaggtt ccgctatcgt gtttcaattc acgcacccgt gagggtgcga
    29701 ccaaggccct ggagaaagct gaagagcttt gcgaagtttc aattcacgca cccgtgaggg
    29761 tgcgactcta tgatgtttat attgttatag ggttgttgga gtttcaattc acgcacccgt
    29821 gagggtgcga cggttgcgct ggtgtaacgc gccaagacat ctgatagaac tacgacaagt
    29881 atcttatata acttgtcaaa ttgactgctc cgtgcagggg gtctgcatcg actctgcctc
    29941 ccccttacaa gcggccagca ggtctcccca gtccactgcc tcgtcgtcct ccctggccac
    30001 tccctcccgg agcatcctct ccctggcgtc ggccagcagg tgcatcactc tggcccgttc
    30061 gctggcgggt tggttggaca tgcgggggct gccggtaatc accacggcaa acctggccgc
    30121 gtaactctcg gcgcttaccg tgcggctggg ccagcaccag gccgtccata tactccacgg
    30181 ggaacgttta cgccatggct catcaatgcg gcacaatgct gccggaaccc tggccaatac
    30241 ctcccgtcca atcgtccaat cttccggggc gcggtcatcc aggagactcc aatccatctc
    30301 ccgataaaga cgtctggcta tccagcctct caatgcgtac aaacacccga agactctcca
    30361 gtctccattg acaaaatctc cggaggtgta catctggcac caggggcgct gctccatcca
    30421 gcgcagccag ccgccatcca gcagagccgt gtcacagtcg accttgacca ggatgtcgtc
    30481 atcctccgcc tcccggcaca tctcggaaag catgccccgg atgcagtcag gcccgcgcag
    30541 gttgccgtgg cgctcccagg aggactgcac atactccgct cccatgctcc ggagagccac
    30601 cgccgtctcc tcccgtaccg ggctggacgc atcgtccacc accgtcacgc tggcacaggg
    30661 cacggccatc cgggcgcacc gcacgcaagc caccgcctca gcggcgtctc cggaatacga
    30721 aaaaataaag attctgatca tactattatc tatagggtaa aatttagatg gcggggccgt
    30781 atgggtaaaa agtgccgtga ttgagcggaa tctggatggt gcccagcatg tgctggtaga
    30841 cctctttgtc ctgcaccgtc gcaatgtgga aagaatagga aaattcctct gactcgtccg
    30901 ccaactcgtc cgtaagaatg agagggttga ctgtaccctg ctcatacatg atcgacgtgc
    30961 cggtcagttt ccctttgccg ttgaatttga cgtccagcca aatttcaccg cccgtgaatc
    31021 cctccttggt gaaccagccg gaggtgtcgt ctccgggagt ctgtccaatg tacactccct
    31081 ggagcatcag cttgccgcgc cgcatccggt agccggttac ctcggtgttg tcgtcagacc
    31141 agtccagctc cacctgcaaa tcatagacgg gaggcttgtc gggagcctcc gtccagacaa
    31201 tctcccctga cggtcccaga gtcggagcgt ccggggactt gtacgcctcg tcgtcatatt
    31261 gcagggtcag catgggattg tcctgctgct ggtcctgatc cagagtctcc tggccggatg
    31321 ccagctgctg aacccggtcc accatatcct ggagggacag atgcgccgga acgccagtat
    31381 tgaggctggt ggacttgccg gcgagatcga ccgtcacccc ctgtacggag gtagccatct
    31441 ccaggtagtc ccggcgcgcc ccggtaatcg ccagacggcg accaaccagg ttggaaggag
    31501 agagcgcccg cagggagttg acgcggccct cccagggcat ggcgcgggtg atctcataat
    31561 attcccgcag aatggaagag tagtcgggcc aatccgtggt ggaggacggc ggcgagcctc
    31621 cgccgctgga agggggattg ctgccgtcat ctcctccgga gtcgccggac ttgctgaccc
    31681 ggtagcgcat gcggctcttg ttgatggtgc gtccctgcca gcgcagccag ttgtaccagc
    31741 gcgttttgcc gtccacctgt ttggtgtgcg ggaacagcat ctcgcacccc ctgggaggcc
    31801 gggtgtccgt gtacaggtac tgtttcagct ccacgtagca ccacttgatt gtcttgcagg
    31861 cctcgctcaa ctggccgctt acatgctcat aggcctgcgc cgactccgcc gtggaataat
    31921 tgctcatgtc ggttccgtcc acgccggcca ctacagattt tttgatggtc cccagctgca
    31981 gtcctgagac ggatgacagc tgggatacct tgctgaacca ccattttctg gcgtttgaat
    32041 ccgtaccgga cggcaatttg acgccgcgga cctcgacaat cggcttggta aagtcccaga
    32101 cgggagactc gctgccgacg ggagcatcat cctccgtcga tgtggaagac agcgctacct
    32161 gggtagtgac gcatccctcc tggcgcaggc tggcccccct gggccagacc tgggaccggc
    32221 aagcctgccg tcctgccgtc aggacgacgc ccacggccgg aggcaccaga tccggccggg
    32281 gagaaagcga gatggcagag aggcagtctt gcgcccggtc cagcgtgact acgggcaacc
    32341 cggccccgtc ggccaccctg atcacggggg aggagccgga gtaatcgacc cagcacacca
    32401 tgccgggacg gcctgccagc attttgcgga gcaccccggc atacatgtcg cagccaagcg
    32461 ccgtgtccca catccaggcg gagggggata cggtcacctc aatgccgacg ccggagggaa
    32521 taagcccgta cttgcgggcg tcctccagcg cccaccgcac cgtgccggca atcttgattt
    32581 tacgcggggc atccgcgccg ctgccgccgc tgacctcggc ataggcggat acgccgcctc
    32641 tcaacgtgcc cccaggattg aaacacaagg ccgcctccag gggctggaga atatcgcaag
    32701 cctcaatgtt ccagcgccag gcgtcgccgg actgatccag ggagcacttg cggatcgtgc
    32761 cctccaggat ggtcacgcca tcccatacca cgcgcaccgg ctctttatat agatagggag
    32821 ccgtctcatc ctgatctctg gccaactgct gccaggtcgc tctggcggcg gtaaagttgc
    32881 tccactcgta cttggctccc ttgatggccc ggtttgtcag ctctacaact ctcatagggg
    32941 gggatgtgat gattaacgac cgaatctggc gcgggactgg agggtggcta cctcctgctc
    33001 catttttctc agccgcgcgt cgtactggga ggcctgggaa gcagactgcc gcgcgataac
    33061 cagaaattgc tgcatgacgt ccaggatgcg cccgctggtc tccccctgtc caaacatctg
    33121 ccgggcaaac tcgccgatca tggcgttgta gccctgaccg gttgcaggct ggggtgcggg
    33181 ctgcggatga ggctgcggag cctgcgcacc ggcgcggatc cgcgtgcgca cctcatcctc
    33241 ggcatccaga tcgcggccct ccggagtgac gcgccggggt ctggccggtt gtggtctgga
    33301 catggtcgtc aggatacggc ccagctcgcc ttggaccagt tccctgacca gggagacgac
    33361 gcgctgcccg gcggcatcgt ccgcgttgat gcgcatgctc aacgcctcca gctgcttgac
    33421 ggtggcctca ttgaggacgc cgtacttggc cacgtccttg agaatagtca ctattttggc
    33481 agccgctcca tctttgggta ggctgggggt gatgtcgtcc aggttgtcca gcttgccctt
    33541 gaaccggcca gcttccagat ccttgcgctc caggggagta agcaggtttc gctctccgga
    33601 cgcggctctg gagtaaccct tgatcatgtc gtccaccatc cacttgacct tggctctcag
    33661 ctccctgggc aatcccttga gggcctcgtc acgcatccgg gccagacgcg cggagtcctg
    33721 ctcggacata tagccggcat cggccatgtg ctgcccctcg gccagtaggc gcttgagctg
    33781 cggagtctcc cggtccttat agtcctgacc ggttgtaagc tggtcgtaat acttcctcca
    33841 tctctcttgt acgccctttc gctggctggc cagctcggcg aggtctttct ggcggcgttc
    33901 gtcggcggcc acggcgctag cctggtcggc cttggcggca gcgattgccg cctccttgga
    33961 attgatttcc ttctggtact gcaggagctg ctgctggttc gtgacgttgg actcgtccag
    34021 tttaagctga tcctcagcat ttttaagacg ttctttccga gctattgtgc tggtttcgtg
    34081 ttccttgatg gcattggaga tatccacctt catcgcctcg ccgccgtcgt ggtctggaga
    34141 atatcgcttg aggggattgt tggcacgata atacccctca atcgctgcaa ttttgttttc
    34201 aacctgttgc cgttctgact gctgggcttt gtctttgtcg ataactacac tggcggaggc
    34261 tatctctttg ttgatgcgtt ccaaccgggc tcggtcattt tgtaattttc gctccgtctc
    34321 atagatggct ttttccgctc ccaacaacgg attcatccgc tgctgcctgt tgagttcctg
    34381 cgttttttcc agcagattga ttctcttctc cagctgttgc ttttccgttt cccattttgc
    34441 cactttcggt aaaagagcct tggcctcctg gtgatgctgc cccaacttct cctcctgatc
    34501 cttgagcgtg agataaccat ctcctgtcat gccggcatag ggggatttag ccgcctgctc
    34561 ctgctcggcc ttaatcagct ggacctgctt ggcagtctcg tcgcgggcct ggatagcggc
    34621 atccagcatg atctgctggc ggtccagacg ggacttggca gcggatttgg cctcggaggc
    34681 gttcaggtca gccatcaggg cggcagccgt ttctttgctg atttcccccc tgatctcgcg
    34741 ggtcttgaca atggtgcgct gcagttccag ctccttctgg cggagggcat ccacatggtc
    34801 cacctccgcc gtgcggttga cggccaactg gccgatgagc tggatgcgct gggtgtaaag
    34861 gtcgttaatg gccgtgattt tgcgctgctc ctcgtcgtag atgcgttcgc gtttggcatt
    34921 ggtcagcgcg tcagtataag cctgctcgcg cgcggcggcc tccctggcca gccggtcttt
    34981 aagttcccgc gcccgtttga cggcggcctt gtccacgccc tcaatgtatt tttcccagcc
    35041 gtccaggatg gcctgtcccc aagctacgcc ccggccgtag gcagcctcca gctcgttggg
    35101 gatgttgaac gtcgcctgta ccttggcctt gaggtcgccc agaccagccg ctgctttcct
    35161 ggctccccgt tccattttgc gcccggctgc ttctcccttg cttcctgcct tgtccatttc
    35221 atcgccggtg gctctggccg tctgtccaac cttactgacg gcctcctgca gcccctctgc
    35281 agcgtcgctg gtcttatcca gagcctcggc agtttccccg gcggcatccg tcacctcctc
    35341 catattgtcg gcggctgcat tgtcgcccag ctcgtcgttg acctgcttgg ccgtcctgcg
    35401 gaccttgtcc agccccttgt tgacctgatc cagtccccgc gtgtcggcct tggcttccac
    35461 acctacctgt accttgtaat ccttgtctgc catggtgata tgatagttat tagttaaagt
    35521 ttatagatgg ttaagaaatg tctcccgtca gagtgaggga tacctgcagg gcggcccagg
    35581 ccttgccctc catgccgggg aggcgtatgt cctccggacg gcggcccata ctcggaccgt
    35641 ccgcgccgag atcgtgatcg ctcgtgagag gcaacggctg ggcaagatcc accgtagcgt
    35701 gataggttct ggtccggccc ggacggcccc ggaaatagca ggacaaccag gtaacggctc
    35761 cctccgggtg aagggtgagc gtctcttgga ggtcgagccc ccaggcacgg gcgcgtccgg
    35821 acgtcgtaaa tgcccgcacc accgtaaaag acatctgcag ggaggcgttg ccccttgacg
    35881 cctggtgcat ccagggactg ccgatgacga actcgcgctg gagctggacg gagggcttga
    35941 cctccaccag ctccgccatc acgtcgtcgt actggcacag cacgatatcc cggaggccgt
    36001 ccggacggta aatgaccgta tcaa
//
LOCUS       DAWEMX010000008        35089 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_498_length_35089_cov_14.207513, whole genome shotgun sequence.
ACCESSION   DAWEMX010000008 DAWEMX010000000
VERSION     DAWEMX010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 35089)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 35089)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35089
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_35089_cov_14.207513"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7850 a        10737 c         8970 g         7532 t
ORIGIN      
        1 atcacgcact tcccgcaggt ggcggccctg gcctcccatc attacctggt tcagaaagct
       61 tccgcgggca accgcaccat ctcctgcctg cgggaggtga gccatgaaga acgggtggat
      121 gaactggtcc gcatgctggg cggcggcgga gaccacgcac gcacgctggc ccaggccctg
      181 ctgcagccct cctgaacggg aaacgccggt gggggagaga ccgccctatt tccccaggct
      241 ccccaggcgt ggggtgaaac cccgcagcct cagggaattg aatacgacgc acagggaact
      301 caaactcatc gccccggcgg caatcatggg attcagcgta aggccgaagg cgggatataa
      361 acacccggcg gccaacggaa tgccgatgat gttgtaaaag aaagcccaga aaagattctg
      421 cctgatgatg agcagcgtgg tgcggcccag ctggacagct tccgccacgc ctgcggggtt
      481 gctcttcatc agcacgatgt cggaggattc catcgccagc tccgttccgg acttcatgga
      541 aatgcccacc cgggcgcagg ccagagccgg ggcgtcgttc acgccgtccc ctaccatcgc
      601 caccttgtcc ccgcgttcct ccatcttcat cacatgggag gccttttcat ccggaagcac
      661 gtcggagacc acctcgtcaa tgtggagttc tccggccatg tgccgcgccg tggcggcgtt
      721 gtcccccgtc agcatgacga cgcgcaggtt catccgcttc aaactgtcca ccgccgcacg
      781 gctgtccggt ttcagggaat ccgccaccat gatgacgccg gcagggcatc cgcccctgcc
      841 cacatacagc ggggacgcgc cctgccgcat gaactcccgc aggcggcttt catcctcctt
      901 ccaggaaatt cccttatcct gcatgaatcc ggaattgccc accgcataag gcaccccatc
      961 caccgttccg gcaatgccgc ggccaggaac cacggataaa tccgccacgg cgcggacagg
     1021 caaattcaga agcctggcgt gttcataaac agcctttcct acaggatgtt ccgaaccctg
     1081 ttccaaagcc gccgccattt ccaccagcgc gtccgcatcc atgccttcct ccggcagcac
     1141 ggccactacc cgcggctccc cttccgtcag ggtgcccgtc ttgtcaaaaa cgacggtatc
     1201 cacgcggctc agggcttcca gagctccggc ggacttgcac aggatgccca gccgcgcgcc
     1261 cctgcccgtc cccaccataa tggcgatagg cgtagcgagc cccaggacac aggggcagga
     1321 aatcaccagc accgcaattg ccctggcgag ggcgaaggaa aacccctccc cggcagccat
     1381 ccacccaacc agcgatacca gggcaacggc aatgaccgca gggacaaaga aatagcatac
     1441 ccggtccgcc agccgcgcaa tgggcgcctt ggactggctg gcctgttcca ccaggcggat
     1501 catgcgggcc agcgtggaat ccccgcccac gcgttccgcg cggattttga gatacccggc
     1561 ccggttgaac gttccgctga tcacccggtc ccccgccgcc ctgtccaccg gcatgctttc
     1621 ccccgtcagg tccgattcat ccagagccgc ctgcccctct tcaatcatgc cgtccaccgg
     1681 aatccgctgc ccggttttca ccactaccag atccccggaa cgaatttcat ccagcggaac
     1741 ggcgcgctcc gcgccgtcat gccataccag cgcctcctgg ggaaccagct tcaccagccc
     1801 ttttacggcg gcattcgttt tccgataaga gcggcgttca aggtatttcc ccagggaaat
     1861 cagggtgacg atcatagccg cagattcaaa atacagctcc attccctcca cactgaaacc
     1921 ctgccacatc cccgcaacca gcaaatacag cccatacaga aaagcggagc cggaaccgat
     1981 ggcgatcagg gaatccatat tgggagacag cgccacaagc tgccgcacgc cgtttatcaa
     2041 ataatgccgg ttcagccaca ggacggcgcc cgccagaatg ccttgtgtcc accaattcat
     2101 ccagagcccc caactccctt ccgggacggg ccagttccac atggggccca tggacaaata
     2161 catcaaagga accagcagca gaatggacca aacgaggcgg gagccgcccg tttcctcctg
     2221 ttccagcctt tcctccctct ccgttccggc aagcggctcc tccttccagt ccgccaggtg
     2281 gaagccggct ttctccacca ccggagctat ccgctccatg gcaggggaat caggtttttc
     2341 aaacaagacg gtcatcttcc ccgtcagcaa attcagctct acgcgctcca ccccctccaa
     2401 tccttccacg gcgcgctcca ccttggcggc acacccggcg caatgcatac cggaaacaag
     2461 atagttcttt tcttccattg tcattaaaac ggaatgtttg atgttccggg tccagacagg
     2521 accccatact gttagccgaa cctaacacat ttattccgtt cctcaagccg ctttcgcggc
     2581 ttcagccccc tctgataaaa aatgacaaaa aaacgcccgc ggccccgtac cagtaacaaa
     2641 cacctttact ttcttccatg aacattcctc ctctattcaa catcctggcc agcgcggcct
     2701 tgttgtccac ccccctccac gcccagcttt acaccctcca tggtgacggc gtcaaaaaaa
     2761 cggagagagt cgtggaaaaa gcggattatt ccgattatga actcgtctgg cacgacgaat
     2821 tcgacaaaga cggccgtccg gaccctgcca aatggaatta cgaacacggt ttcgtccgca
     2881 ataaggaagc gcaatggtac cagccggaaa acgcctattg caaagacggc atgctgatta
     2941 ttgaaggcag gaaggaaaaa catgccaacc cccattacga tccggaggga aaaaactggc
     3001 agacggcacg gaaagaggcg gaatacacct ctgcctccct gacgacgcgc agcaaatttt
     3061 cctggctgta cgggcggttt gaaatccggg ccagattcac cccgcgggaa ggcatgtggc
     3121 ctgctttctg gaccttgggc gtcaaggaaa agtggcccat gtgcggggaa atagacatca
     3181 tggagtacta ccagtccacc tatctggcca atctctgctg gggttccccc aaaaaacacg
     3241 tgggcaaatg gagcaccact tacacgccgc tcaaatggct ccaggaaaaa cacgcggact
     3301 gggcggacag cttccatgtc ttccgtatgg actgggatga aaaggaagtg cgcctgtatg
     3361 cggacgatat tctgctgaac cggactcccc tggataatac cgtcaacgcc tcttacaagg
     3421 aagtcgccaa tcccttccgg cagcctcact tcatcatcct gaacctggct ctgggcgcca
     3481 ccggcggaga tctggacaaa ctgccgctgc cccagaaata cgaaatagac tacgttcgca
     3541 tttaccagaa aaaggattcc ccccataaag gaaccattcc ataccgcgcg gaaaagtaac
     3601 cgccctcttc cggaaacagg aaaaagggca gggattgcgg catgaaaaaa gccccatccg
     3661 gacaaaccgg acggggctct tcaaagaaca gaaagagggg caactcagtt tacgcggccc
     3721 agataggaac cgtcttccgt ctttacggaa atcttttcac cgggcttgat gaaaaggggc
     3781 acctgcacca ccagtccggt ggtcatcgtg gcggatttgt aaacattgtt cgcgctgtcg
     3841 cccttgacgc cttcaggagc ttccgccact tccatggtga tggcggcggg gagttctacg
     3901 gaaacgaccg tttcatccgt gaaaagcagg atgtagatat tgccttccat cagataatcc
     3961 ttgacgggtt ccacgatttc cggagacacg gtgatgtctt cgtatgtatc cggattcatg
     4021 aagtgataac ccatgccgtc aatgtagctg aactcatatt cctcacgggc gaggttgacg
     4081 ccttccaggg attcattgga agtcaggcga aggttataca cctttttagt ggaaatgctg
     4141 cggatgctca tttgcacata ggaagccatg cggggggggc acttgtgctc catgctgact
     4201 acaacgcaaa cgtcattgtt gtaattgacc gcgtgtccct tgcgaagatt gattacgggt
     4261 acttttgcca tgcggcccgt accataccat gttttcccct cccgtcaagg cgaaaaaacc
     4321 gggcaacccc ttgaggcgct tggagaaaac gctcctgaac agggcagcgg gaaagaaagg
     4381 gaaaacggct gtttctaccg gctgaggtcc tgctggaaca ggactttgat gccgctggaa
     4441 ttcagcacgg tgcgcccgaa gttgagatcc tccacgtgaa gagccaccag agacccacgc
     4501 cccacgcaag gcagcagggg atagagaaag ttgacgttca tttccgcggc atacagcgca
     4561 tccaggcatt ttttgaggtc tgcagggcca tggcggagca ccgccaccag caccccggac
     4621 tccgtatagc aaataccttt ctccagaaaa atttccgacg tcctttccgg atcgctgaca
     4681 atcaggcgcg caatagtcgc ctcatggcag tcctgcatgc tgaatcccag acagaaaatg
     4741 ccgtgcatgt ccagcaggcc gaggagggcg gtcagggccc ccacccggtt ctggagcatg
     4801 atggaatatt gctttatcgt gctgccgggg gctttaatgg cttcttcctt cataacaata
     4861 aagcaaataa gggccgtatt ctatttggcg tacgttttaa gcacgcgcct gatgatggcc
     4921 agagcctgct ggatttcctc ttcactgaca ttcagcggcg gcagcagacg gacggtttcc
     4981 ggcccggcgg gaacggaaag caacccttcc tgccgcaggg cctccaccac tacggaggcg
     5041 atcgtcttgc cctccggcgc ggccaccatt tccggattca ggccaatgcc cagcaacagg
     5101 cccaggcccc gcacgccctg aacaacaggc aggttccagg attctatttc cctgcgaatt
     5161 tccgcgccca ggcgccgcgc ccgctccggg agcccctggg aaacaacttc ccgcagcacg
     5221 gccaggccta ccgcagtgcc gagcgggttg cctccatacg tggagccatg ggagccggga
     5281 tccatgatgg aggaaagctc cgtaccctgg ccgtcaatgg cgcggtcgga aatccagaag
     5341 ccgccgatgg ggaaaccgcc gcccagagcc ttggcccagg aaatgccgtc cggttccaca
     5401 tccggagcta cggcgcgcca tcccatgatg tcgccgcagc ggccaaagcc gcactgcact
     5461 tcatccatca gcagaagaag atcgtgctcc ctgcacaact gcgccaggcc ccgcagaaat
     5521 tccggagtca cgcagttgac ccctccttct ccctggacgg tttccagcat gaaggccacc
     5581 gtttccggcc tgatggcttc gcgggccgtt tccagatcgt tgaaaggcaa atggcggaat
     5641 cccggcagca tggggtcaaa gccgattttg attttatcct gccccgtggc ggacatgctg
     5701 cccagcgtcc tcccgtggaa ggatttgttg aacgtaagca cctcataacg gggcttgccg
     5761 tccggctggg gcttgcggtg cccgtaacgg cgcgctactt tgattaaacc gtcattggac
     5821 tccgcaccgc tgttggcgaa aaagactttt cccgggcgct ccacgcattt ggtcacgatg
     5881 aattccgcca gatccgcctg ctgggggatc tggtacaggt tggagcagtg caccagggtg
     5941 gcggcctgct tttgcagggc ctgcgtcacc gcaggatggc agtgcccgag cacgcaggtc
     6001 gcaatgccgg cacagaagtc cagatagcac tttcccgtgc tgtcccacag tcgggagcct
     6061 tctccgcgga cggcagccat gggataacgg ccataggaat ggagtacgta ctggttgagc
     6121 ttttcttctg tgttcataag ttaaggtaat atgggcgtag tttgctcttc tggccccgtc
     6181 tgtaaatgat ttttttggga accacgtcat gattgccgca ttttaccccg gaagacagtc
     6241 acatctttgt ccgccgccca ttcatttttc ttttctttta tcattttaac ggcagaataa
     6301 ccaccgcaac cagcaataat ccactatggc tcattttttc aacctcaaag gctttctgga
     6361 agaattcaag gcattcgccc ttaaagggaa cgttattgac ctggcggtgg ccgtcgtcat
     6421 cggcggcgca ttcaacaaaa tcgtctccgt cttcgtttcc agcatcatca cccccatcat
     6481 cggctacctt acttccgggg tcaacctggc atacctggtg ctgaagctgg gcgatgtgga
     6541 attaaaatac ggggaattgc ttcaggcgac cattgacttc ctcatcatcg ccttctccgt
     6601 ctttgtagcc atcaagctga tcggagccct gaggcgcaag agcgaagaag ctccggccgc
     6661 ccctgctccc aagccggatg acgtcgtcct cctggaacag atccgggaca tcctgcaaaa
     6721 acaggcggac gccaaataag cccttttgaa cggttcatgc cggttccata cgggaccggc
     6781 atattttttc ctctttactt gttccgccgc agacgggaaa gtgattgcat gacctctccg
     6841 ctgcgccttt tagcctttgc cctccttctt gctccgtccc tgcttccggc ggcggaaaac
     6901 cagcgcttca ctctggaacg cccctatccg gtggcggaaa tcccggaacc tgaccgcagc
     6961 cttcccgtca ccaacgtaat cctgatgatt ggagacggca tgggcatcca ccacctttcc
     7021 gccgcatggg cagccaacag aggacgctta tttatcgaga actgcccggt tacgggtatt
     7081 tccaaaacat ggtgcgcgga caagctggtc acggactccg ccgctgcggg caccgccatg
     7141 gcgacgggaa ccaagacgct ttatcatcga gtcgccgtat gccccaaggg caacaggctg
     7201 gattccctgg tggacaaggc ggcggacatg ggcaaatcca ccggcgtcat cgccacctgc
     7261 gagctgaatg acgccacccc ggcatctttc tgcgccaata atgaaaaaag atcggatgcc
     7321 tacggcatca ttggggatta cccccgttcc cgtgccgatt tcatctttgg cggcggcggc
     7381 aaatacttca cgcagaggcc ggacggcagg gatatcttcc aggaaatgca ggcgcaggga
     7441 taccgcgtcg cccgcacctg ggaagaagcc ggcgccttgc ctcccggaaa aacgctggcc
     7501 gtcaccgctc cggggaatct tcccccgccc tccaagcggg gagacgtttt ccgccaagcc
     7561 gcactgaaag ccctggacac cctttcccgg aatcccgacg gatttttcct gatgattgaa
     7621 gggtccaaca tcgacaaggc agcccacagc aacaacctgg aagaaatgat ggaagaactc
     7681 cttgattttg accggactgc gggagccgtg cttgactggg cctccaaaca ccccggcacc
     7741 ctggtcgtca tcacggcgga ccataatacg ggggcattcg cgctcacggg cggcagccgg
     7801 gaaaagggtg aaataacggc ccggttcggt tccggcagtc atgacggcgt ggctgttccg
     7861 gtgtacgctt tcggcgcggg aagcggtgct ttcacaggca tttatgaaaa tacggccatc
     7921 ttcagcaaga tcatggatgc ctttaaaaaa ggtgcgggca agcccgtgcg ttaggtaccc
     7981 tgcggcctgt ccatgcgccg cgccaccagc ccggccccgg cggtagccac gccgcctatc
     8041 aggaacaccg cttgcgggcc gcccgtattc atccagatca cgccgcccag cactccgtaa
     8101 gcgatagaag ccacatgatt gatggccagc ccggtgtaaa tgcttggcgt aatgtcttcc
     8161 ggcttctcgc agatgcgggc cacgtaggtg ctccgggcca tacccagggc aaacagcagg
     8221 ttgtccagga caaagcaggc ggtcactacc cccacggccc aatgcagggg cagcagctcc
     8281 ggcgcgaagg cgtatgccag acacaggagg gacagcgcca cgctgtcgaa aatcgtcacc
     8341 ttccgttccc cgtagcgctt gatgctccag ccgataaggg gttgagtaac cagtccgatg
     8401 acaccggcca tgagcagggt ttttccgata tatccgggag gctggcccag cgtgctgacc
     8461 atcagccaga aaccaaaggt cagataaagc tgcttacgga tgccgtgcag aatttccaac
     8521 ccataataaa cggcatacct gcgcttgaag acgaaacatt gcctccagcc gcgccgctgt
     8581 gggaagacgg gcgtcttcac tcggctcagg aacaggaacg ccagcaggca cacgcccccc
     8641 gtgacgaaaa agtccacggc aaaagactgg ttgaatttcc acttgatcca cacgcacagc
     8701 gcccccagca gcgcggcagc cgttcccaga gccttcatct ggccaagccg caggctgcgg
     8761 ttttccggcc ttgccgtatg aatgacaatg gtatcaatca tgcccatcag gatatgctgg
     8821 cccaggctca ccgtcagaat ccatgcggac agggtccaca ggctggttcc ggcgccgcag
     8881 aaagccagcg cgtacatgcc gccgaacatc agcaggcacg ccaccccggc cacgcggacc
     8941 tcattaaaca tgaaaagcag gctgaccaca aaaagagaca ggatgccggg caattcccgg
     9001 gggaattcaa tcagcccgcg catctccacg tcaagatgct gggcatcacg aaggaaattg
     9061 ctgaaaacga gatcatacac accgcttccg aactggccca gaaaaagcgc cagcagcaca
     9121 aataccatcg tcttcctgat gcggggggaa gcggctccat tcatagcgcg ggaatactct
     9181 cacaggcgcc cggacggaac aaccgctatt ttcatgtgcc ggttttctct gcatttgcaa
     9241 aaataacctc gaaacggaat tttcccggaa aatgccgcgt atttacgtac gctatgattt
     9301 ccaagtgtac cttttccgcc acggttttca gcctgttttc cctttgctgg ggcgccccat
     9361 ccgctccggt tctcgaagcg ccccatatca ttcccctgcc cgccgccatg cgcgtccaaa
     9421 ccggagaaag cgggttttcc ctgaaaaacg gcgtcaggct cccggaaaaa aatcctcttt
     9481 ccaggcaggc ggaacggatt ttccgcgaca acgggatcaa cacggccctg gttaaaaaca
     9541 acgcggacat catctttacg gaagacgctt ccctgggcag ggaaggctac cgccttgccg
     9601 taacgccgga ttccatctcc attgcctccg gttccgtgaa cggaaccctg tatgcccttc
     9661 aatccctcgt tcaaagcatc gctgccgaca aaaacggagc tccggccctg ccccggatgg
     9721 acgtaaaaga ccagccccgc ttttcatggc ggggcctgat ggtagacagc tgccgccaca
     9781 tgatgcccgt gcgggacatc aaaaaagtgc tggacctgat ggaacggtat aaattcaaca
     9841 ccctgcactg gcacctgacg gacgaccagg ggtggcgtct cccgatcgcc aagtacccca
     9901 ggctgacaac cgtgggaggc gcccgggctc aatcccccgt catcggcaac cgcaataagg
     9961 gagacggcat cccctactcc ggccattaca ccgcagatga aatccgggat gtggtgcggt
    10021 acgccagaga ccggggcatt accgtcattc cggaagtgga aatgccaggc catgcctccg
    10081 cagccatcgc cgcctatccg gaactgggga atacggacat cccgggttat gagcctaggg
    10141 tgcaggaaac ctggggcgtg cactcctata ccttctcccc cacggaaaaa accttccgtt
    10201 ttctggaaga cgtcattgat gaaatatgcg ccctgttccc ggacagcccc tacatccaca
    10261 tcggagggga tgaagcgccc aagaatcagt ggaaacagtc ccccacggcc cagcgggtca
    10321 tgaaggacaa cggcctggcc aatgaacacg agctccagag ctacttcatc cgccgcgtgg
    10381 aaaaaatgat caataaccgc ggaaaaaggc tcattggctg ggatgaaatc caggaagggg
    10441 gcctttcccc caccgctacc atgatggttt ggcgcagcca aatgccgcac atcgccgcac
    10501 aagccctggc tcaaggcaac gatattgtga tgacgcccaa cagccacctg tactttgact
    10561 atgaccaggg gcccggaaaa cccgctgccc ccgaatacga gacgattaat aacaatcagc
    10621 tgacctggca gcatgtttac ggactggaac cggtgcctca gggaacgccc cgggaacggg
    10681 aaaagcaggt gctgggctgc caggcgaaca tctggacgga atatatcccg aacctgccga
    10741 aatgggaata ccatgtcttc ccccgcgccc tggcgctggc ggaagttgcc tggaccccgc
    10801 aggagctaaa aaatgagaaa gatttccgta aacgcctcga ccgccagctt cccttcctgg
    10861 acgcccgcgg cgtcaattac aaaagaccgg acaatggagc ccccgcacag ccggaggccg
    10921 tcattacgcg ggaacgccgt taagcacggc agacgggttc ctgcggcacc tccttccgga
    10981 ggccgcatta tgggctagcc aaccttcatg aaaaccatta cattggcggc attatgaatt
    11041 tggaactgct tcgtcaggtt tgcgtgactc cgggcgctcc ggggtttgaa gacaaaatcc
    11101 gcgatttcat catccaggaa gtggccccgc tggtggacgc cgtgcgcgtg gacaacatgg
    11161 gcagcgtgat tgccatcgtg gaaggcaaaa acacggaaaa aaccatgatg gccgccgccc
    11221 acatggatga aatcgggttc atggtccgtc acatcgacga caaggggttc atcaaattcc
    11281 tgcctctggg cggctttgac gccaagacgc tgacggccca gcgcgtcatc gtccacggta
    11341 aaaaagacct catcggcgtc atgggcgtga aacccatcca cgttatgtcc ccggcggaac
    11401 gtaccaagct gccggaagtg accgacttct tcattgactt gggcatgagc aaggaggaag
    11461 tggaaaaata cgtttccgta ggcgactccg ttacccgtga acgggatttg gtggaaatgg
    11521 gggattgcgt gaacgtcaaa tctctggaca accgcgccgg atgctacgtg ctgattgaag
    11581 ccctccgcgc catcaaggct tccaggaaga aaccctcctg caacttcgtg gccgccttta
    11641 ccgttcagga ggaagtgggc ctgaggggcg cgcaggccgg cacgctggac atccaaccgg
    11701 atttctccat tgccctggac gtcaccatcg cctgtgacat tcccggaact ccggcgcacg
    11761 accaggtttc ccacctgggc gcaggcgccg ccatcaagct gtatgacggt tccgtcattg
    11821 cagaccgccg catggtcaag ttcatgaagg ctatggcaga cgccaacaaa attaaatggc
    11881 agacggaaat gctgccggcg ggaggcacgg atgccggagc catgcagaaa ttcgttccgg
    11941 gcggttccat tgccggggcc atttccgttc ccacccgcaa tgtgcaccag gttattgaaa
    12001 tggctcacaa agacgacctg gacgcttccg tagcgcttct gaccgcctgc gccatgaacg
    12061 tggacaaatg ggactggtcc tggaactccg tcaacgaatg cccggcggaa aaacccgcca
    12121 aggccgcaaa aacggcagcc aagcccgcca aagccgctgc caagaagaaa aaggccaaat
    12181 aacaggtctg gccattcatt catcactacc cggcaggccg caccggaaac ggaggcggcc
    12241 tgcctgtttt cagcctcctt caaatgttcc ggcaaactcc ggacaccgtc ctctcccatt
    12301 cagccaggca taccgcaggt tcctgcgtca ccccccggag gctttttcag acggccctgc
    12361 cataaaaaaa tcctccggca tctcgcaatg tcggaggaga gacaatcctg aaaaaaaagt
    12421 tacttggcgg cgggggcgga cgcttccttc tgggcgggag cgggggccgg aactgcttct
    12481 acctccacat cttcctccac ttccacatct tcttccggac cggcagcgcc ggtctgcttc
    12541 atggcgtcct tcagggcctg gcttccgtaa aagtcggcct gctgcaaacg ctgcatgtca
    12601 gtcatcatgc ccatgaaaat gggcagaagc tcggcctggc gcgcctgcat ggcggcatcc
    12661 atttcagcct gaaccttctg ggcgctcttg gaaagggctt cccccttttc attgattttt
    12721 gccatctttt ccgcagccgc gtctgcagtg gccttgtcct tcaccgtgct gagcgtggcg
    12781 gacatttcct tcatcaacgc cagcatctga tcagcggctt ccttcgcttc cttggaaact
    12841 tcagcggccg gcgcaacggg cgcggcagga gtttcagcgg cgggtgccgg agccggggca
    12901 tcctgggcgg aagccatacc tgcaaatgcc agggcacatg ccgaactaat tgttacaatg
    12961 ctcttcataa cgtttccgtt gtacaggctc gccatgcacg cgtcaatgaa tcattgtgta
    13021 acggctcccc gtgatgccgc agaaagagga atttgcgttt tttcctccct tgaaggattt
    13081 ttttagttgc atctttttag aattattata aatttatttc cgatgactaa gaccatagaa
    13141 aacctgaaag ctgccattac gggcgaaagc accgcctccg ccacttacgc ggcctatgcc
    13201 gccaaagccg cccaggaagg gcagcccctt attcagaaat tgtttgaagc cgcctccaag
    13261 gcggaatccg tccacgccgc caaccacaac aaggttctgg aaaagctggg ggcaacaatg
    13321 gagcccatcg atatccagat ccatgtagga accaccgcgg aaaacctgaa agccgccatt
    13381 gccggggaaa cccatgaatt tgaaagcatg tatccggaat tcatcgctgt ggcggaagag
    13441 gaagggcaga aagccgccgt ccgttccttc acgtgggcca cggaagcgga aaaagaacat
    13501 gccgattttt attccgtcgc cctgaaggcc ctggaaagcg gagataccag ttccctgccc
    13561 aggcaatact ggatatgcct ggtttgcgga gatacgttca aatctctgga aggcgtggat
    13621 gcgtgccccc tctgcggcac caaggctgaa aaattcctcg tcatcggata aattccgttt
    13681 gagcaccgga ggccgtccac ggcacccagg aaccgcccgg aacattccgg gcggttttct
    13741 gtttttgccg cagcagtcca tggaagaaaa cccttccctt tttacggagg cggcccacac
    13801 cgggaaaagc ttctccaaca ttcttcttgc gattcatttt aaaactctta taatttttct
    13861 tcgctttaag caggaagcat tctttatccc tcctttgcga tgatccctcc ccatgcatcc
    13921 gccggttccg cagaacggta tccgtccgca tacggcctgt cggccgctgt ctgccaggct
    13981 ctgcgcgccg cgttcattcc ccgccgcatg cgctgcctgt gggcagccgg gcttgcggcg
    14041 gcgctggcgg ccaatcccta tgtgctcaac gatggccaaa gcctgctgat gagcagcatg
    14101 ggcatcgcct gctccgcatc cctgctctgc ggcaccattc ttctctgcct gaaataccgc
    14161 ttcaccgcct atgtccttct tcccgccatc attctgttca atgcgggcct gtacatgatg
    14221 cagatgcggt acggattggt cctgaacctt tccgtgcttt ccagcatggc ggagaccaat
    14281 ttccaggaag cctgcgcctt ctgcaccccc gcttccattg caggaaccct gcttctggcc
    14341 gggtttatct acctggccat ctactggagc cgccgttccc tgcgccaaaa agccacgtgg
    14401 ggcgcactgg catgcatttg gggcctgtac gcggcccttc tgctgctgag cattccggca
    14461 gcctcctaca gcttggaacc cctttatctt tactacacgt ctgacaaggc caaaggctgg
    14521 cctctcgtgg atattgcaat gacgtgcaaa ctggcggacg aatacatcac ccaggatgcg
    14581 gggcgtttca acacattgcg gaaccttccg tcctgcgcgg agcccctttc ccagtgcgaa
    14641 gccccagacg acctggccgt ggttttccac atgggggaaa gtgtccgggg ggaccatctt
    14701 cccctgaacg gttaccatcg gaacacaatg ccccggcttt ccagagaacc caacgtcgtt
    14761 tccttccccc acgccacttc cttcggcatc gtgaccagaa tttccgccat cggcatgttt
    14821 acggatgcgg aactgtgccg ccgcactccc ggtcactcct ccttcattga cctgttcaac
    14881 aaacacggat tccgcaccgt ccgtatcatg gacctgagcg gagattccat tcatgattat
    14941 tccctgggca tcctgacacg gaactgccgt gaacggagac agacgccgct ccagcaccag
    15001 acacccggaa tgatgcagga acgaacttcc ctggtcatgg aggaatccct gaaaaacttc
    15061 ggccgcaaca ggcagcttta tatcatttac aataacggga gccatatggc gttcagctat
    15121 cccgcgcagg cggaacgttt taccccggca tcctgcaata tggacgaccc caaggcccgt
    15181 ctggaagaaa ccgtcaatgc ctatgacaat tccatcgtcg acctggatgc ctccatccac
    15241 cgcatgatcg cactgttgaa gaacaggccc gccatttatt tttactgctc cgaccacggc
    15301 gtagcactgg gagaggaagg aaaaatgttc cagggccata tcctgccgcc tgtttaccgg
    15361 cctgccatgt tcatctggta ttcggacgcc ttcgcctcac gctatccgga catggtgcgc
    15421 gccctgaaag ccaaccggct gaaagccgtc tcccacgacc acatcttcca tacccttctt
    15481 tccctggctt ccatccggtc ggaaatcgtc aggaacgacc tgaatctggc ttctccggac
    15541 gcgcgggaaa ctccggcccc cctccagccg gaaacgctgg cggaatggca gcccattccc
    15601 gcaccgccgc agccgtagca aaaggaagcc gccacgcgct ccgcgagaaa caacaggatg
    15661 cggcggctcc taccgcaccc ggacttgcag aggatggccg atgcactccc ggacacggcg
    15721 agcctccgct tcccagccgg cagcggaccg aatgggagaa gccttgtccc aaaccgcccc
    15781 catgtaccat tcaaagggtt ctccgttcgc aaccttcctg agcaagtagg tacagccttc
    15841 cgtatccacc gcacggcctt ccggaaccca gggcacgatt acggcggttc ccaggcatcc
    15901 gtcatccttc cggggcctgc tccatgcggt catcaggccg ccggactccc ggtcctccat
    15961 aaccgcttca taatcatttc tgtgctttcc ggtatccatt cccacaccgg ccttcaccgt
    16021 ttcagtcccc cggacgttca agacgctgcg gactttggag aaacggtttc ccgcatccag
    16081 cgtcaccctt cgcgtctccg ccacacgcac gccgccgcca atgtcccacg gtgcataaac
    16141 cacctcaaaa gcagtctgga ccgggccatt gtacaatgtc cgggcgctgg cccagtttcc
    16201 agacacatgc ggttttccat ccctgaacac ggcaatgccg ccgcagcccc gccccggacc
    16261 tacattgtac atatccagtc cccaaccgtg atcccggtgg taatcccccc gtttgtaaaa
    16321 ttcattgata acgggagcac ccgcgcgctt gctccataca tccgtcccac tggaaaccag
    16381 gccctctccc tcaggggctg gcctggcgac ggccggaccg taaatccggt gcgccgtaag
    16441 atcattttcc cacgcgaaat catccagccg ctccggtacc ggacggctga aacagacatc
    16501 cgcggaaggc gcttcgccca agcaggaatt ctccagaatc cagaaatcac gaaccgttcc
    16561 gggccggaaa ttgctctgga acaaaagggt atccgttgtt ccgtcaccat ccgtatccgc
    16621 cagctgatgc gggataacac atccgtgacg gacatcaaaa acgcgaaggc cggcggcatc
    16681 accggaacct ccggacggcc atggaaccga cacagtctca gcagggcgga acctgcccag
    16741 gggattcgta acggtcacga tcttcttctg cggatgatcc cggccaatga catatttacg
    16801 caactcactc ccggccatca ggtaggcccc tgcgccgtac acctccgtat ctttcccgga
    16861 ataatggccc ggcttgtccg caataggctg cacccagccc agcgcgccct ccttgctgac
    16921 cgcatcacag gcggccttcc aggccttacg gatggaggga aggtattcat ccgcatccag
    16981 atatccctgg ttcactcccc acagcatccc gtacatgata aaaagggtgc cgctcatctc
    17041 tttcaaggga ggttcgtccg gatccagcag gctcgcatgc caggaaccgt cgggcgactg
    17101 gcattttttc agggctgccg ccagacgttt aagcaagtct tcataaaagg ggcgggaagg
    17161 ccagtccgca ggcatgtcct gcaaaataag aggcaatccg gcaatcaccc agccgttgcc
    17221 ccgactccag aacatctttt tcccatttgc cgccggaacg gtaaaatagc gggaatcccg
    17281 gaaaaacagg ccctcctccc ggtcaaaaag atagtcgcag gtaagcttgt attccctatc
    17341 cataaactcc agataacggc ggtctccggt ataggccgcc aattttacga acacgggagg
    17401 ggacataaac agggcgtccg accagctcca gcggtcctgg cagcccggag tcaggaattg
    17461 gagggaagcg gaggaaggcc ggttcatcac cttgtccagc acggcgcgga tcttttcagc
    17521 tgcggcggga ttgccgtctt ccatcgccat ctccatccag gcatgcccca cggcatggtc
    17581 atccgcatga taatgccggt tcagggtctt ccacccctgc tcttctcccg cttttctcag
    17641 caacggcagc cccggccctt ccggaacgga caggccatat tgcaccaggc cggaataaaa
    17701 ggcaccgtaa gtccaatgca gcggcgaacg ttttttggga tgcttcgcct gccattccgc
    17761 caccagctga agattatccc ggatggtctt ttcattccag tctgcggaag ccccggaaac
    17821 ggaagttcct gcggaaagca tggaaaggac cggcaacaga aagtatttct tccatccatt
    17881 ttttgaaaag gcaaagaaac gcatgttata ttatacggga aaagaaggct ggaattttct
    17941 tgtaacaaga attcttcttt tcccgcgctc ctccagaccg ggaaagggac gcaagggcat
    18001 atatggtgcc atgacggaaa agaaagctgt gttatcaatc atttttctac cgtcatatac
    18061 tatatatagc atatgaataa ttatttaaca cattatttat acatttttct tgcgggaggt
    18121 ctctttctgt ggttcattgc gttccagcgc aggtaacgga agacggtgaa aatccgtcgc
    18181 ggacgcgccg ctgtaaccgg tagacgatgg accggacact ggctgcccag gcagccggga
    18241 agtccccatc acatgtcgca ccggaagcca gaagacgacc tgatgctgcc gtattcccat
    18301 cccgcggaca ggatgaacat acgcacaggt cttcggcatg cccccctttc cgggaatgcg
    18361 cgcatgcggt cggcccctgc ctatggtttt tccaggcttt tgcccaggct cgcggatgag
    18421 cggtcaccac cctttccaag cgagacaccc atcatggcaa caagcatcat caaacgttcc
    18481 gggaaaaagg aacaatacca gggctataag attgaagagg ccattagaaa agccttccac
    18541 agttccggga ccgcctacaa ccgttccatc tatgaagagg tggaagccaa gctgagccat
    18601 caggacagcg cgcatgtgga agagattcag gacatgattg aacgcgccct gttccgttcc
    18661 ggatattttg acacggccaa ggccttcatc acctaccgtt tcctgcacaa aatgcagcgc
    18721 gaacagcttg gcggccttta ttccggcaat acctatgtgg actgcaagca gacggtagag
    18781 gaatatatcg ggcaaagcga ctggcgcatt gccgccaact ccaataccac gtattccaat
    18841 gcggggctca tcaacaacac ggcgggcaag gtaatcgcca actattggct ggaccaggtt
    18901 tactccgcac gggaaggagc cgcgcaccgg gaaggggatt accacatcca tgacctggat
    18961 tgcctgaccg gatactgcgc cggatggagc ctccgcaacc tgctcaatga aggattcaac
    19021 ggcgtgcgca gccgcgtgaa cagcagaccg ccccgccact tccgggaagc cctgggccag
    19081 atggcgaact ttctgggcat tctgcaaagc gaatgggccg gcgcccaggc cttcagctcc
    19141 tttgacacct tcctggcgcc ctacgttttc aaagaccaga cggacttccc ccgcatcaag
    19201 aaagacatcc gcagtttcgt ctacaacctg aacgtaccct cgcgctgggg acagtctccc
    19261 tttaccaatg tcacgatcga ctggactgtg ccgcgcgacc tgcgggacca agcccccttc
    19321 agcggagggg aacacctctt tgaaggaata gaagacgcga acctgctcgc cctggcgagg
    19381 gaacgcgggg caaacaagct gacggaactg acctacaagc atttccagaa ggaaatgaac
    19441 ctgatcaaca aggcctttta cgaagttctc acggaagggg acagcaccgg acagcccttt
    19501 accttcccca tccccaccgt caacatcacg gaagactttg actgggaagg ggaaaacgtg
    19561 cctctcctgt ttgaaaacgc cgctaaaatc ggttcctctt acttccagaa cttcatcggc
    19621 agccagtaca ccgtcaatga acacggggaa cgcgtgcctg acgaacgggc ctacaagccg
    19681 gatgccgtgc gttccatgtg ctgccgcctc cagctggacc tgcgggaact gctcaaacgc
    19741 ggcggcggtc tcttcggctc cgcggaaatg accggctcca tcggcgtagt gaccatcaac
    19801 ctggcccggc tgggctacct gttcaagcaa aaccgggagg ccctgttcgc caggctggga
    19861 gaactgctgg acctggccaa atcctgcctg gaaaagaaaa gggccttcgt tcaggaaatg
    19921 tatgaccgcg gcctgtaccc ctacaccaaa cgctacctgc ccaccttccg caaccatttc
    19981 tccaccatcg gcgtcaacgg catgaacgag ctgttgcgca atttctccaa cgatacgatg
    20041 gacattacct ctgaagaagg cagggccttt gctgaagaga tcctggaatt catccgcttg
    20101 cgcatgcagg agtaccagga ggaaaccggc aacctgtaca acctggaagc cacgcccgcg
    20161 gaaggcacca cgcaccgctt cgcccgcgaa gaccagaaac gctatcctga catcatccag
    20221 gccggccctg tggggcagcg ttattacacg aacagctccc agcttcccgt ggaccacaca
    20281 agcgatctgt tccgggcgct ccagcttcag gatgaacttc aatgctgcta cacgggagga
    20341 accgtcttcc acatgtacat gaacgaagcc atcagctccc cggaagcctg ccgggacatt
    20401 gtccgcaaag tgctgacccg cttccgcatg ccgtacctca ccgtcacccc gctcttttcc
    20461 gtttgcgaga agcacggcta tctgcgcggg gagcacgaat actgcccctt ctgcgatgag
    20521 gagctgctcc acatccacag gcatgaataa aatccctttt ccaataggct cccgacttca
    20581 aacccaacca ttaatacgac aatgaccaaa caggaaatat taagcaaata cgcacaggac
    20641 cggacccgct gcaccgttta cacgcgcgtc atgggctatc accgccctgt agaaactttc
    20701 aacgccggca aacagggcga gttccatgac cgcgtccact tcgtggaacc gggccgcccc
    20761 tgctcctgct ccgcccaaac ggaacagccg cgataattct cccaacaaca tatgcgaagt
    20821 cgatgggaac gggtcggggc atagcagaca tcacgccgct caccttgctg gactttccaa
    20881 ataaggtggc ctgcatcttc tggctgagag gctgtaattt gttttgtcag tactgttaca
    20941 acgtctccct ggtcagggga acggattctc ctgccggaga ccggacggac tatcttgatt
    21001 ttctcagaga tcgggtggga tttctggatg gggtggttct gtccggaggc gagtgcacac
    21061 tgtgtcccga ccttatccct atctgccgca atatacggca gcttggcttc gcagtaaaaa
    21121 tagacaccaa cggcacccgg cccggcgtgg tgaaaacgct ggtggaggaa gggctttgcg
    21181 attatattgc cctggattac aaagcgccgg aaaaactgtt cggctccatt accgggcgtc
    21241 cggatttatt cccctgcttc actcaaacgc tggattatct gatcaacaga aatttcccgt
    21301 ttgaggttcg aacgacaatc cactctggcc ttctcgggga aaaggaaatc aaccagatca
    21361 gcggggattt gacgtcgcgc ggctatagag gaacctacta ccttcagaat ttttttaata
    21421 cagaggaaac actcggccaa atcggcactc ccgaacggat gattgactta tctctgctaa
    21481 acacgcatat ccccataggg ttgcgcaatt ttcccatcac ggagaaagct cccggctcca
    21541 gctccgggaa acggcagccg tcccctctga catgattttt ttaaataaaa gtttctattt
    21601 gtaaaatcgg gaagtaccct tccgcccgcc aaccatatta ccagctatta ttcatgagtg
    21661 aaacccaaaa caagctgctc aaggcagcta tcagcctggc cgccgcagga gtcgtcttct
    21721 tcctgccttt cgcttcatgg ggaatccaat taagccctat tgaaatccgc gtcatcgcca
    21781 tgttcgtcat ggcggcgctc ttctggattt tggaacccat ccctatctgg accacctccg
    21841 tgatggtcat taccctgtcc ctgctgtgcg tctccaacgg ctctctctca ttcttgatgc
    21901 cggagcgcta tgacaaggcg gcggtatcct ccattctgga tgacgccata ggcaaaggga
    21961 tcaatccgga ggtagtcggc aaattgaagg aaaatgtcga aaaccggtta aataagaaaa
    22021 ccaagctgga cgcggaagaa gtgaggatga ccctggggtt ccagctcatg gacgcttatg
    22081 aaaaaataga cttgaacgca caggaactgt cccgtgaagg aaaaacggaa gaagccgccg
    22141 ggcaggaatc catagccgcc cagctcaaga ccgcggccgg gcgcctgtac agcaaggaaa
    22201 taaccgcgcg tatccagggg ctgcaatttg tcaacaccat gcagcaaaaa tccacgatgg
    22261 ccaccttcgc ggatcccatc atcatgcttt tcctgggcgg ctttttcctg gctgcggccg
    22321 caaccaaata caggctggac atgaacctgg ccaaagtact tttaaaaccc ttcggcacca
    22381 atcccaaatt cgtacttctg gggctgatgt ccgtaacggc tcttttctcc atgtttatga
    22441 gcaacacggc tacggccgcc atgatgctcg ccatcctgac gccggtgctg gccctgttca
    22501 ctccggaaga caaaggccgc gccgcctttg ccctggccat ccccatcgcc gccaacctcg
    22561 gcggcattgg aacgcctatc ggcacgcccc ccaacgcaat cgcgctgaag gcgctgcagg
    22621 gtatggggct ggacgtctcc ttcggcaaat ggctcatgtt cggcattcct ttcgtcatcg
    22681 tcatgattct gattgcatgg cttctcctgc tgtggctgtt ccccatctcc caaaagaaac
    22741 tggaactgca ggtgggaggg aaattcctga gaacccccaa ggccattatc gtttacgtca
    22801 cctttgccgt taccgtcctg ctgtgggtaa cgggcaaggg agtacacggg ctggattcca
    22861 ataccatcgc catgattccc atcgccgtct ttgccattac ggaaaccatc accaaggaag
    22921 acttgaagaa aatgggctgg gacgtactct ggctggtagc cggcggcttt gccctggggc
    22981 tggccctgca agacaccgga ctggcaaaaa acctgatcgg ctccattccc tttgccgaat
    23041 ggtccccctt cccgctcatg gtgggtacgg gaatcatctg cctgttcatg gccaccttca
    23101 tgagccatac ggctacggcc tccctgctca ttccgattat cgccgttgtc ggcgtcaaca
    23161 tgggtgacaa cctggctccc atgggcggcg ttaccgccct gctggtatcc gtggccttcg
    23221 cctcctccct gggcatgagc cttcccatca gcacccctcc caacgccctg gcctatgcca
    23281 ccggcctggt cagctccaag ggaatggcca tttccggcgt catcctgggc atcctgggca
    23341 tggttttaac ctacgtcatg atgatgattc ttgcccaatg ccatgctttt taagtaaggt
    23401 aatgttccat gccgggacat gttgccccgg catggccaac atcaacggga aaagcgccct
    23461 atgatcattg aacaggaaga attccagaaa aaactaaaac gcatcaatct ggcgtacttc
    23521 tccgatccgg accgcattat tgccatcagc aggggaaaca gcgtcgtccg tcagggggaa
    23581 cagaacaaaa ggctttacct gattttatcc ggcagcgtgg tttcctaccg ccacgtcgtt
    23641 aaagaagacc cggaaacagc ttccacttcc cacaaaatct acaaggcgtt ccgggccggt
    23701 cccggagact atttgggagt gcagagtttc ttttccggat ggttccgcag ctcctccgat
    23761 ctcattgcgg aaaccgattt ggaactggcc tatatcgaca ccgccacccc tgccgtggac
    23821 gtggaacact acggcacgct cctggaacag tttgttcccg ccattgtcca tgaactggcg
    23881 gcgcgcaata cccgcgtctt tgaccgcact gcggaaaagg aagaagcgct gcggatgctc
    23941 caccgttctg aaatggccgc caccctgggg caattatccg ccggaatagc tcatgagctc
    24001 aacaattccc tgggagtcct gtcccgtaaa acagactttg ttctggaaac cctggacaac
    24061 atgttccgcc aggaaaacgc cttccgctac accctgtaca acaaagggtt ggaaggggct
    24121 tcctccgcat ccacttccga acagcgcagg ctggcgcgcc agtttgaaaa aaattacggg
    24181 ctttcccagg acgtggccaa ggccctggcc ctgattccgg acatacagaa tcagccggag
    24241 ctgctgaaca agccattcct cagccagctg gaacaaaacc tcccttattg ggaattgggg
    24301 cacgacctgc atgacatgaa actggctgtc aggcacgcta cagccattgt ccgttccgtt
    24361 cgcctgctgg gaggaggcaa caccagacgg gaagaagggg taaacgtgac ggattccctt
    24421 acggaagctg ttgctctggt caagtccagt ctgcgcggcg ttgattttga aatgatcctg
    24481 cctcccagag actccgtgcc cggcatcttc gcagatatga cggagctcgt tcaaatttgg
    24541 atcaacctgc ttcagaacgc ctgcgacgcg ctgaaaatag aaaatacccc caacccttcc
    24601 atccacgtag aatgccgggt ggaaaaaatc agcagcaagg accagacatc ccataccacc
    24661 cgtgaaattc tggtctccgt cacagacaac ggccccggcg tcccaaagga cctgcatgaa
    24721 aaaattttcc gtcccaactt cacaaccaaa aagaaaggtc tttccttcgg cctggggctg
    24781 gggctcacca tcgtccggcg cattgtggac agctacggcg gccgcatccg cctggagagc
    24841 gttccgggcc ataccgtttt tacgatagtc ataccaacca tcaacaatca tggaaaaaat
    24901 taacatcatt tgcgtggacg accagccgga agtcctggac agcgttttgc gcgaccttcg
    24961 tccgctgaat tcctgcttcc gcctggaagg ggtggaatcc gcatccgaat gccgcgagct
    25021 attggaagaa ttcgaccagg acggagaact gacgggactg attatttccg accacgtcat
    25081 gccaggaggt tcaggagtag agttgcttgg aggcatcgcc aaagacgacc gttttgccgg
    25141 gacacgcaaa atcctgctga ccggccaggc cacccacgcg gacaccatcc aggccgtcaa
    25201 tgacgcacac attgacaact atctggaaaa accctgggat cccgccgttc tccttgcgac
    25261 cgcccgcaaa ctgctgacca ggttcatcat ggacaagggc atcgaccata tgccctatat
    25321 gtcctgcttg gaccagggta ctctgctgga ttacctgcgg agaaactgac ggggctggaa
    25381 ggcatacgcc gcaccctcta ctttttcaac gcacgcactc tcatccgtga cgggcattcc
    25441 cccgcccttc gtcaaggaag gaaacaaaac cgctaaccgt ctttatgttt gatatcctgc
    25501 tccatcataa caccctggtt cttttcggaa tcatcttttt cggcattgct ctcggcagca
    25561 ttaaaatata cggactgaac ctgggccttt ccggcgttct tttcgtggct ctggccgccg
    25621 gccactaccg cctggccatt cccgacggcg tagggcagct gggcctggcg ctctttgtgt
    25681 actgcgtggg cctcagcgcg ggcaaccgct tcttttccgc catcgccaag cagggcagca
    25741 aactggccat catgtctgcg gtgattgtag gagtgtcagc tttattcacc tgccttttcg
    25801 gcatctggtt caaagtcccc gcgggaatct cttccggcat ctttgccggc gcatgcacca
    25861 gcactccggc gctggcggcc gccacggaaa gcctgccggg agcctccgga agcggcgtca
    25921 gcatcggtta cggcatcgcc tatccattcg gcgtggtggg cgtggtcctt ttcgtccagc
    25981 ttctgccgcg cctgctgcac tttgacctca acaaagaggc caaagccatg cattccaaac
    26041 aggaggtcag catcatttcc cgccttgtcc gcgtcacaca gcccaacctg ataggccaaa
    26101 acattgcgga ctgcaagctg ctggacggcc tgcactgcat ggtcacccgc gtggttcgca
    26161 acgacaggct gatgcccctg actccggaag acacgttccg ggaaggaacg gaagtcctca
    26221 tcgtgggcaa cagcgacact atgccccacg acatcgcctt tctgggggaa gtatgcgaca
    26281 atccctaccc catggactcc cagaaggagc gccgcaaact gattctgaca aacaaaaact
    26341 atgccgggca caagctccgc gacctgaaaa ccctgcgtac ggacggtatc attatctccc
    26401 gcatttcccg cctgggcttc actttcgtcc ccacggggga aaccacactg gaacgcaacg
    26461 acgtgctgac cgtggtgggg tccccggaaa accttaccaa atacggggac aaaatcggcc
    26521 accgcagcca ggacatgaac cagacggacc tgctttccct gggcttcggc ctctccctgg
    26581 gcatcattct tggcctggtc aacttcagcc tcggcaacgg cgcgggaatc tccctgggta
    26641 ttgcgggagg ccccctcatc gtcgccctgc tgctcggcca cttcggacgg ctgggcccca
    26701 tcgtcggcta catcccgcgc cccacccgcc ttcttttgca ggacctgggg ctggtcttct
    26761 tcctggctga cgccggcatc aagggcggag cggacttggt ggatacggta gccacccacg
    26821 gcgcggccgt cttcatcatg ggcgttatcg tcacctgttc cggcatgttt gccggttact
    26881 tcgtaggcat gaaggtcttc aagatgaaca tcctggaatg cctgggcggc atctgcggcg
    26941 gcatgacctc caccccggct ctgggagcca tcgccagcaa aacggactcc caggctcccg
    27001 tggtcagcta cgccaccgcc taccccgtgg cccttatcct gatgaccatc atcgccaagg
    27061 tcatcatcca gaccatcggc ggcgtggtgg gcatctagga ctgccagcaa atccacagga
    27121 actcaataaa gaataaaggc ggcaataatt cttttattgc cgccttcatg ttagtgcccc
    27181 ccttaacgca actgccccaa tatccggttc agctgaaaag ccggaaaggg atcatgcatt
    27241 ccgtcacggg cgtaccgttt acacctgcca tctacccctt atgaagaagc ggcgtcagaa
    27301 tggcaagttg accgcataac tcacaatatt aacaaagcgg ggatgaaacc tgcgccaaac
    27361 accggttccc accatgaaaa aaaacatcag gctgcaggca aaaagtctac agctttttta
    27421 tcatgaagcc ggagcatcag ggtttgtttc caccacctcc aggctgacgg actggaaggc
    27481 ctttttccag cggatgcgga tgccggcgtc catcagaaag tcgcctccca gccttcgttc
    27541 gtgaacatcc gtgagagagc cgtcttcatt catgttgatt tcccgcacca ggtacctggc
    27601 ggaaggattc aggcctttca ggcgcagggg cggaggggaa tccgccagcc acttatccat
    27661 cagccaggcg aaaacgacag cccgttttcc gtatacatac atcagggagg ccacccggct
    27721 ttcatacggg gaggaaagcc ggtacaagtc accgaactgg actacggggc ggatgcgctt
    27781 gtattccgcc agagcccgtt tggaaaaaac catgtcctcc tccgtcatgt cacatggctg
    27841 gagttcaaaa ccaagccggc cggacatggc cacgtcaaac cggaatttga gcggagcgga
    27901 gcggcccgtc tggtggttgg gggaggcggt cacatgggcg gccatggaaa tagccgggaa
    27961 caggtggcct atgccccact gcatgaacac gcgttcataa gcgtccgtgt tgtcggacgt
    28021 ccagaattca tgatgcttcc gcatggttcc atagtcggcg cggcctcctc cggaggaaca
    28081 ggcctggaat atcacatccg ggaattccgc cgccagcgtc tcaagaatac gttcatagcc
    28141 ccgcacgtag tcaatgggca gattcccctg gcggcaggca tccagccagg gagagccggg
    28201 gtcggaaatt ttgcggttgc aatcccattt gacataggaa atacccggat gccccccaag
    28261 cagcttccgc atggcgtcaa gaatgtacct gcatacatga ggattgctca gatccagcaa
    28321 atactggctg cgttcctgcc tgttttcccg atgcgggaga ccgatgaccc actccggatg
    28381 gttctgataa agctccgaat gagggttcac catttccggc tccacccaga tgccgaagcg
    28441 tattcccagt ttttccgcct ggcgaatgag accttccagt ccatgaggca atttagcgcg
    28501 gttaacttgc cagtccccca atccggcacg ggcgtcattg cgggggaacc ggccgccaaa
    28561 ccagccgtca tccagcacga aaagctctat ccccaagtct gccgcacgtt tcatcatgcc
    28621 gtgcagcact ttttccgtaa aggtaaaata caccccctcc caggaattca gcaaggtcgg
    28681 cctctctgat tctccaccgc gcaaaccgta acggcgcgcc cagcggtgga tgcgccggga
    28741 ggcctctccc ttgccgtttt tactgtgcgc cagaatgagg ggcggcgtct tgaacacacc
    28801 gccgccgtcc agcagatagg gcgcgggagc catatccagc cccgctccgg caaaaaggta
    28861 atgatacggg ctgtgccgga accatatccg gtaattgccg gaccatgcca gtgcccccag
    28921 aaccacctcc ccggaatcct cccgcgccgg accgtccaga gagatgatga atccggggct
    28981 tccctcctgg gcagtgcggg tgccggttcc ggataccaaa gcaagttcat gcccccgggc
    29041 cacttcttcc tcactcatca gggactcgcc gccccaggtg ccgcggaagc tggtgacgaa
    29101 ataccgttct gcgcgcaaac ccagctgtgc ggatgccgcg cggtgcaggc ggatgccttc
    29161 tttcccttcg ttccgaatca ccgcccattg cagaaaagtg tccgattccc tgtgggagcg
    29221 cacgtggatt tccacccgca cagggtagga agggtccttc ccatagaaga cggcttcttc
    29281 ccggtcgtca tccagttcca gcacttccca atccaggcat tccaggctca gggaaagggc
    29341 cccgtccgcc tgggttacgc aaatgcagta ctcgccggac gcattgggaa gccctgttac
    29401 ggaatccgcc agggaggaat acaggagagg agcatccgcg gcgttttcca gggcatcctc
    29461 cggttcacgc agcttcggtc cgtaataggc gcggcgcaca cggccctgtt catctttgta
    29521 cagggtcatc tgagaggaac gggtcagcaa atgaacggaa tccggaatca tgatcggcaa
    29581 atgcggaaag agccagttac ttattcaggc aggcaaaaac gctcctgcct tatccacatt
    29641 aagggcctcc acggacgggg aagtcaatgg caaaatggca ggagttcccg agcgtttccc
    29701 ctactccttc cacagaaggg aagaggggcg gaatttcatg gttttcgatt catgcaccag
    29761 cagcctgccg ccgttttccg gatggcgtac gatgcgggcg cggcgccgct tgacgcggaa
    29821 agttccaaag ccccggaatt tcaccagcga ccgcctccgg actgcccgcg caatcgtctc
    29881 cagcaccgcg ttcaacgcat gttccgcctg ccgggaagta acgtcattcc ccagctcacg
    29941 ctggagctcc cggataaatt gtgttttatt cataggcctc ccagtatgag gcccccgttt
    30001 ttccggtaaa gcggcaacgg cgcaccctca ttcctgccac tatacccata caaacgcccc
    30061 cttccattca ggcctgcgag tttcctcctt gcccccgcgg gccagcccca ttaaggttca
    30121 ggggaaatgc ctgcctctgc cgctgatgcc ccgtgcccgg aacaagcgtt caacgcaact
    30181 gcgctgaagt ggcacacctg cgcctggctt ctgctgcccg tcctggtatt cctggccggg
    30241 tggatgcact ggtacgccgc tctccccctc atgctcctga cggctgtggg actggcaccc
    30301 cggaaacgca aggaaccgca aacaagggat tcccttcccg ccttccccct gttcacccgc
    30361 tcttcatttc tcgccctggc ggcgtttgcc gccctgatga cgctttccgg ctggggggaa
    30421 tgggtcaacc agcaccctga ccatatcgtc agaaacgcct gcctgcgtga attggtagcc
    30481 agcccatggc ccgtcatctt tccggacggg aacatgctgg tttacaacac ggggttctgg
    30541 ctggtcccag ccctggcggg aaaactggcg gggctggacg ccgcacgcgt gtttgtcgtt
    30601 ctgtggggaa cctgggggct gtttcttacc tggctgtggc tctgcgtttt ctccggacgc
    30661 cgctccctgc tgctcgccct gctgatggcg gcattcggtt ctctgctgaa tttccagtgc
    30721 tggctggggc tggacctgtt ccgcctgcat tacttcggca cggcggaaca aatcatgtgc
    30781 agcgcaaacg cttccattcc agttctgctt tttttcatct tcctggcttc gggacgcatg
    30841 cccctgccct ggatacccct tctgttcgca gccacggcct tttattcccc cctcgcggcc
    30901 ctcggtgggc ttcccctggc ggcctgccag tggtaccgtc agtggagggg cgtcccccct
    30961 ctccgggatg tcctgctcca tccctccttt tcggcctccg ttctgctggg agtgtgcttc
    31021 cttctttatt atgggacggt aaacgcgcca tcctcccaca tgggcatgcg gcctttttat
    31081 acgcatccgg gggatttccc gcttatggcg acctcattcc tgctgtacgc cctgttctgc
    31141 tggcgggatt tccggaggaa tcccctcttt atctgtactc tggcaaccgg cctgattctg
    31201 cctttttttt acgtgaacgg agacgtcaat gacctgcttt gcaaaggaag cgttccggtt
    31261 atggcctgcc tgcttgcctt cctggcttcc tcctggacgc ggcatcccgg tttccgcgtc
    31321 tggatatggc ttctgctggc gttcggaatg gcaccccggt tcaacattcc ctattattgc
    31381 tgttcttcca gcgccttcca ggcgtttctg gcgcctccgg cttcggaaac agagccttct
    31441 tctttcacca gccgggagtg gaagcagttc ttttatgcgc cccaggcgcg ccgtcaagat
    31501 gaatggggcc gcaccatgca ccaccccgga caccggtggt atccctacta ttccggcacg
    31561 tcgcgcccat ggctgcgctt catttaccgt ttttagaaga gtaacacctc atgatgccga
    31621 atgagggcgg caaaatcggg caacttgcac gtgtgcaggt attaaatttg acctccgatg
    31681 tccctaaact tttcaatgaa agcgacgatt ttttgaaaga cgctctgttt cttcattaga
    31741 tattgagggt ttaacggact catcttcggc agaatagcat tgaggtcagc gccgttctcg
    31801 ctggcgtact tccgtttcaa agatgtttga atatatcgct ttgcggcttc ctcgttcagt
    31861 ttctcttccg taatcagagc atcagcttcc tttttctgtt tctcctgggc ataagtaaag
    31921 aaagcggcaa taatatcagt cttatctcgc atcgattcca aatctgtttc attgataaaa
    31981 tcaaccagga ggctctcttt tgccctgttc ccgatgctcg ctctgataac acggcgcacc
    32041 tcatctacca atgcaggctt gtccttcgtt ttcctgttgt gttcgaaaat cagttcgagg
    32101 atatagtcga gatctatttc ctgagattta agcagatcaa tttcaaagac cacatcatcc
    32161 cagtctattt ttgaatcttc cggagcttta ccgttcctct cacgtctgaa ccagtcccga
    32221 atatcattgt acgccgaacg ataatcctgt acggttcgct ctttcagtac atcaatgccc
    32281 tgcatggctg caatatcttc atcagtcaag aagttggcat tcttaaacgc ttcaagagcc
    32341 gcactatccg ctttgtctat tttttgcagg gctttgagct gcgtaaactc atcgtagttt
    32401 tgtaaaatat tttctatccg caaatattct ccgaacagct taacaaagga ttttttatct
    32461 gcttccgtca ctatcccatc gggggcaggg aatttttctt tcagttccct gacaacttcc
    32521 acataccctt ttctcacctc gccggtggca atatctttaa atccttcaag atattccttg
    32581 tagctctttt caagtactac gttccttgtg ttcttatctc cgaacaaagt gatcgcatct
    32641 attgtagatt gttccaaatc cctgaatgtg acgatattgc cgaaagtctt ggtcgcgtca
    32701 tagatgcgat tggtacgtga aaacgcctgc atcagaccgt gataacgcag attcttgtct
    32761 acaaacagcg tgttcaaagc aggagcatca aaaccggtaa ggaacatacc gaccacaatg
    32821 agcaaatcaa cctcctggtt ttttactctt ttggccaaat cgcgataata gttttggaac
    32881 tctttactat ccactccgaa gttcgtttta aacatcacgt tgtaatcgcc aatcgctttt
    32941 gccaaaaact ctttggcgct gctgtccatt gccgaagggt caaaattttc atctttgata
    33001 tcgcccttag cgctttgttc ttcattcggg gcgaaagaaa atatggtggc aattttcagg
    33061 ggcctgtcac tttctttctg tagattgttc aattcctgat aatacagttt ggcagcctca
    33121 acgctgttta ctgcgaacat ggcattaaag cccttgttat tcccttgcgt tctatgggtt
    33181 tttatcctga aattctgcag gatgtattga gaaatccctt tgatccggtc tggatgaagc
    33241 aaaagctttt gattttctgc cgcattcaac ttgatttcat ctttttcagt ttccaaattc
    33301 ttgaaccgtg gacgaacatc gttgtagtcc actttgaact tcaaaacctt ttcatcacgt
    33361 atggcgtctg tgatgacata cgaatgcagc tcacggccga acacactggc cgtagtctcc
    33421 gcccccaaag cattctcagg aaagataggg gtacccgtaa agccaaattg gtaatatctc
    33481 ttgaatctct ttttcagatt cttctgcgct tctccgaatt gggaacggtg gcactcatcg
    33541 aaaataaata caacctgttt ttgataaacg ggcaaatcgc tttcgctttt cataaggttg
    33601 ttcagcttct gaatggtcgt aacgataatc ttgtcgttat tcttctctat gttcctcttc
    33661 aatcctgccg tgctctctga cccattgacg ctatcgggcg agaaacgttg atattccttc
    33721 atcgtctgat agtccaaatc cttgcggtca acaacaaaga acaccttgtc gataaaatca
    33781 agttgtgtgg caagctgtgc ggccttaaaa ctcgtcagag ttttgccaga ccccgtggtg
    33841 tgccagatgt atccgcctcc ctccggattg ctccatttct tagcctgata agaacttttt
    33901 atcttccaca agatgcgttc cgttgccgca atctggtagg gtcgcataat aagcagcgtg
    33961 ttactggtat caaacaccga atatgtgaaa agaacctgca acagcgtatt tttctggaaa
    34021 aatgtcgccg taaaatcctt taagtcttta atcaacatat tgtccgactt cgcccagttc
    34081 atcgtaaagt caaaactgtt tttatcgcgc tttacggtat tggcaaaata acgggtgtcc
    34141 gtaccattgg agatgacgaa aacctgtaag tatttataaa gtgaactctc gctgttgaag
    34201 ctctctttgc tgtagcgacg cacctggtta aaagcctcgc ggatagccac accccgcttt
    34261 ttcagctcta cctgcaccat aggcaaccca tttaccagta tcgtgacatc gtaacggttc
    34321 gcttgttccc cgaactgctc aaactgatta atcacctgta ctttgttgcg ggcaatattc
    34381 gtcttgtctg ccaagtatat attctcaata tgcccatcat caaacacaaa gtcatagatg
    34441 taatcaccgt gtattttttt tgttttttca acagggctgt cgctcttctt gtccagatat
    34501 tcctccagaa agcgacccca ttcagaatcg gtaaaaacga tattgttgaa tgcctgcaac
    34561 tggtttctcg cattgcagag cagggcgtca ggcgtaacaa ggctgggaac atattcgtaa
    34621 ccttgtgata tcaagtcttc tatgaattca cgttccaaag ccgcctctgt ctgataacct
    34681 gcgggcggct cgcttaccat ggaactcttg atgtagttgt cgagaacgat aaaattttgc
    34741 aattctgcaa tggtgctgta ctgtgtcatg gttttctctc tttccagtac ctgttatcta
    34801 tcagatgatc taataaaagt ctcacagttt ccttttctgc ttctgtcggt tccgctacag
    34861 cttcattgga taatgtagag tggcttgtga actgaataat cctgttatag tagatttgcc
    34921 tgtcatcagg taacaaatca gaccattttg gatatcctaa gaagtttgcc gttttttcgt
    34981 agagattgcg cagtagcgtg aaatggtatt tctgaacagc acctgaatca agcgcctctt
    35041 cgatagtctg cttgaggaaa agatgatagg aaaaactttt gtttgaatc
//
LOCUS       DAWEMX010000009        33892 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_529_length_33892_cov_11.248397, whole genome shotgun sequence.
ACCESSION   DAWEMX010000009 DAWEMX010000000
VERSION     DAWEMX010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 33892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 33892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..33892
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_33892_cov_11.248397"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15201..15210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    29729..29738
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8117 a        10056 c         8574 g         7125 t
ORIGIN      
        1 gcgagctgac aatggatgac atgcgctcca tcacgtccag catcgtggat atcctgaagc
       61 tcaaataagc gaggcccctg ccctcttttc cggctccgtc cggaaaagag ggcggaacgc
      121 ctccgccgga acatgaacgg aacaatcgcc gggaacagga tacatcgcgg atatcccgga
      181 acttgttcat ctttgatctt ttccgcatcc cggcggcatt cgtcatcgtc caaactttca
      241 atcaggcacg gtctttccgg gacaacccgt tctttcagga ctccatttca cgttatttgc
      301 tgctgttcat tgcggcatgc attttggccg ggcttgtttc cagcattcgt gcgctatatc
      361 atccttccca ttactgactt tccgaactct tccttccatg tccactctcc atcccttccc
      421 cgccctgcgc cctcctcggg aactggccgc gcgggtctcc tccctcccct atgatgtcat
      481 gaaccaccgg gaagccgagg aaatggcctc cggaaatgac gcctctttcc tccatatctg
      541 ccgttccgac atcgacacga gcgaagccgc catccatgcc ccggaaacct acgccaaggc
      601 cagggaaaac ctggaaaggt ttgtccgcga aggttacctc ttccgggatg aacggccgtc
      661 tttctacatc taccgacaaa tcatgtgggg gcgcgtacag acgggcatcg tcggctgcgc
      721 ctccgtggat gaatatgcca gcggcgccat taaaaaacac gaactgaccc gcagggaaaa
      781 ggaacttgac cggattgagc attttgacgc ctgctccgct cagacggaac cggtattcct
      841 ggcctaccgc aaacatgaag gtctttccgc catcatccgg gaatggatta aattccacaa
      901 acccgaatat gattttacca cggaggacgg cgtcacccat atcctgtggc ccgtttccga
      961 gcccggcacg gtggaagaca tccgcaaaag ctttgaggaa gtggacgccc tttacattgc
     1021 ggacgggcac caccgcacgg cttccagcgc cgccgtttcc gccaggcgcc gccagaaaca
     1081 cccggactac acgggacagg aagaattcaa ctacctgatg gctgtcgtct tttgtgatga
     1141 agaccttttc atcatggact acaaccgcgt catacgcgac ctgaacggcc tgactgaaga
     1201 ggaattcatg aaaaaactcc gtacctcctt tgacataaat cctgtggata ccattcccgg
     1261 agaaggctac gccccccgcg ccaagcatga gttcggcatg tatctgaatg gacgctggta
     1321 ttccgccacg gccaaaccgg gaaccttccc ggacggccat cccatcgaaa gcctggactg
     1381 cgccatcctc caggcgaatg tgctggctcc cattctgggc attgacgatc cacgcacgag
     1441 cgaccgcatc gacttcgtag gaggaatccg cggtctggga gaactggaac gccgctgttc
     1501 cggggaaatg gcgctggctt tctccctgta tcccgtcacc atggccgacc ttttccgcgt
     1561 agcggacgcc ggagaaatca tgcctcccaa atccacctgg tttgaaccca agttgcgcag
     1621 cgggcttttt atccatacca ttgaacaata atcttttcca ccgtaccaac atgaacaagg
     1681 ttcttattcc taccaaactc tccgacgtag cggccaacac gcttaaaacg gccggctatg
     1741 aagtcatcca ggacgctgat acgcctctgg aaaaacaggt agccgcccat ccggataccg
     1801 tggctctgat cgtccgcagc gaaaaagtca ctccggaaat catggacgcc cttccttccc
     1861 tgaagctggt cgtccgcgcc ggagccggtt acgacaacat tgacattgtg tacgcccgca
     1921 aaaaaaacgt ggacgtcatg aatacccccg gagccaactc caatgctgtg gcggaagaag
     1981 tcatggccat gattctggcc tactaccgcc acatcgtcca ggcggacgcc accacccggg
     2041 aaggcctgtg ggaaaagaaa aaatacatgg gcagcgagct gaccaaaaag acggtgggca
     2101 tcatcggcct gggcaatatc ggccgcaacc tcgtcaagcg cctgcaggga tttgaaccca
     2161 ttctgctggg ctacgaccac tttctggcac gccagcgcgc cctgaacatc ggcgtcaccc
     2221 ccaccagcat tgaagatatt ttctcccagt gcgacattat taccctgcac gttccgggcg
     2281 gcccctccac ccaccacatg gtgaacgcgg agctcattgg aaaaatgaaa gacggagcag
     2341 tgctcatcaa ctgctcccgc tacggcgtgg tggatgaaga agcgctggcc gccgccaagg
     2401 ccgcaggcaa aaacatcggc tacctgacgg acgtacatcc caaggacgcg cccggagaga
     2461 agccttccgc ccccattgcg gacctgattc ttccccacct gggagccaac acccgggagg
     2521 ccaatacgaa agcggccaaa cgcgccgcag aacagatgat cgcctatttt tccgacggcg
     2581 acacctcctg cgtggtgaac ggggaatccc ccaacggcct caatccggcc catcttcaac
     2641 tggcgtttct gctcgcctcc ctggcgcgca aggccggcgg caacaagccc atccgccgcg
     2701 tggaatgcac tttttacggc aacctgcgca ttttccgcaa atggtttacg gcccccattc
     2761 tggaaggcct tctgccgcat gcggaaaaag ggctcatgcc tgccgccgcg gaagaatccc
     2821 tgcgggagca cggcatcgtt ttcaaagcga gggaacccaa agacgacaaa ccctatgaag
     2881 attccatcac gctggatgtg gccatggaag aggaagagga atatttccac accagcgtgc
     2941 gcggcgtggt gacggaaggt atccccatgg tctcccgcct gaacaatttc aacggattgt
     3001 acgcggatct gcgcggcacg acattatgcc tgcgctacaa ggaccgtccg ggtgttatcg
     3061 ccctcatcgg ctccgccctg tcttccaatg gaatcaacat tgacaacatc gccgccccgg
     3121 ccgaccacgc caccagggag gccctgaccg tgattaagac caaccagccg gtttccgatg
     3181 agctgctgga taaaatcgcg aaagagatag atgccatctc tgcgttttcc ctgaatcttt
     3241 aacagggaaa gacgcatttc cggaaacctg cttttcagac cgcccgcttc cttcatctcc
     3301 ggagggaagc gggcggttct tatttctgcc atgttgacaa ggcttaccca tttttatacc
     3361 attgaccgaa tcgtcggaac atatccatgc gctacgtact gcttctcctg accctgttaa
     3421 ccgccggatt ttccgtctgg tactatcaaa accgcatttc cccggaagga aaggaactgg
     3481 ccgcccaaaa aacaacattg gaacaggagg ccgcaatatt ggaaaaagcc gttatccaat
     3541 cccgaaagaa gctggatgat gcccaggatg ccacccgggc ggcggaagaa gacatagaga
     3601 gattccagca agtttatctc caacaaaaaa ggcagcagca tcaggaaatc caggatgccg
     3661 cctaccaaaa aagcgtggcg gaccagcagg aagaagtgaa ggaacacgac caaaagattg
     3721 accggctgcg ccgccgcatg aaaaggcaaa gggaaagcac caaatccgcc agggaggaac
     3781 tgctccggaa aaaggaggga atgagaaccc agcttgacag gctgaccgat cagcagagga
     3841 gcattcaggc ggaactggaa caggcggaac gcgaactggc ccaaaaacgg gaagaggcca
     3901 aaagcaggaa gaaattgtcc gggattgccc ctcagaaggg aaatatttcc gaacttcaga
     3961 cacagatgaa ccttatccgc cagaaaaccg cagctgctaa ggaaaaaatg aaagccgtag
     4021 atgcggcgct ggaaactctg gaagaaaagt ctggcaaaca ggaacgcctg atgcagaagg
     4081 cggaggagaa actcacccaa cagaaaaaca aggttctggc cgacgatgac cgggacgaat
     4141 ccgctgtccc ggaaccgggc gatgaagact tgctggcgtc tgccgaatac agggagctgt
     4201 ccagagatgt acggaaaact ctggaacagg cccgtaaaaa agaagaacag gcatcccggg
     4261 cctatgacga agcccgcgcg gccatgcgga aagtctccag ggaagaagtg aaaaagctgg
     4321 caaagaagga aaaagaccat gactcctttc agaagctgtt cacgggtgca gcctccgtcg
     4381 cgggatttat cctgctcctg ttcacgggtg cggccttccg cagcagcaac agttgaacgt
     4441 ctgccggaaa atgaaatgca cggagaacaa ggaagcaccc gccttccctc agggagtcgt
     4501 tgacggacgg cagggatatg atagtctcag gccagcctga tcatgctcac actggcccag
     4561 attcacggac aaccggaaat tttccattcc attcaggggg aaggagtctc ccagggaaca
     4621 ccctgcgttt tcctgcgtct ggcaggctgc aatctggcct gttcctggtg tgatacggcg
     4681 tattcctgga acggaacggc tcccggagtg cgcctcgcgc ctgaaaaggc ggctgaactg
     4741 gtgctccatt atccatgccg ccgactggtc ctgaccggag gggagccgct cattcagcaa
     4801 aaggcgcttc ccgccctgct gcgcctgttg ccggatcatg ccgtggaaat ggaaaccaac
     4861 ggcaccatca tgccggatac ggaactgctg aaacgcgtca cgcaattcaa cgtatctccc
     4921 aaacttcccc attcaggcaa taacgacgtt aagacttgga aaccggatat cctgcgctgt
     4981 ctggcgggta cggagaaagc ctggttcaaa ttcgtcgtgg cgtgcgagga cgatgtcagg
     5041 gccgttctgc ggcgggcttc cgaagcggac attcccccgg aacgaattct gatcatgccc
     5101 ctggcctcca cacgggatga gttgaacgcc atgcgtgcgc aggcggtgga atggtgtctc
     5161 cgctacggtc ttcgtttttc agaccgtctg catatcgcca tctggaacag caaaaaaggc
     5221 gtttaagcac gctcctgact catcaggaaa gacaaatgct tcccgctttt ccggccggaa
     5281 ttccgcaagc ctccaaggct ctgcgccatc gggaaaccta cgctttctgt ttcaaatcca
     5341 gcagcatctg ggtattgctg gggtatttgt tcaggaaaaa caggaaccgt tccatggctt
     5401 ctacgggttt atgcccggct aaagcgcggc ggatcagatt catcttgtac agccatgctt
     5461 ccggcataat gagctcttcc ctccgcgtcc ccgatttcag aatgtccacg gccgggaaga
     5521 tatattgctc cgcaatgcgg cggttcagca ccagttccat attgcccgtc cccttgaatt
     5581 cctggaaaat caggtcatcc atgcggctgt tggtttccac cagggcggtg gcaatgatag
     5641 tcagggaacc ggcctggcgc gtattgcggg cggcggcaaa aaggcgccgg ggcatttcca
     5701 gagcgcgggc atcaatgccg ccggacatcg tgcggccgct tcccttgtca gcgttattgt
     5761 aggcgcgggc caggcgagta atggaatcca tcagcaggaa aacatgctgc cccgcctcta
     5821 caagccgttt ggcgcgttca atgcaaagtt ccgccatgcg gcagtgatcg cgcacccttc
     5881 catcattgga agaggcgtat acttcagctc cggggaggga acgtttaaac tccgtcactt
     5941 cttccggacg ctcgtctacc agaagcacca tcaggtgaat ggaatccctg taattctcca
     6001 gaatggcttc cgccatgtgc tgaagcagcg tcgtcttccc tgcgcggggc ggagagacaa
     6061 tcaggccgcg ctgaccgcgc ccgattggag caatcagatc caatgtgcgg gtggtgtaac
     6121 gctccggccg ggtttcaaag gaaatgcgct tattaggatt gaccgccttg agctcttcaa
     6181 agtgcgggtt cttgcgcgct ttctccggag caaccccgtt aacggaggca atttccgtca
     6241 gaagaatgcc gcggtcatga cggcaggcct gcccatgcac ccacacggca ggacgcagac
     6301 cgaacctgcg gatcatatcc tgcggcacat aaacggcttc cgcaaaagcg tcaaaatcat
     6361 tatccggctt ccttaaaaag ccgaacccct tattcgtaat ttccagcaac ccgtccaccg
     6421 gttccggcgg cgccagttca cggggggcgc tgttctgtgg ggttccgccc tcctgattat
     6481 tattccagcg gtttttaacc cggtcattgc catttctgcc attgcggcca tcctggcggt
     6541 tattccggtt tttgttgaaa cggtcgttgt tattccggcg gttctgcctg tcaaaacgct
     6601 gcctttgcgg ctcatttgtc cgacgcggct cccgggcggc atcttcggaa aaatccgccg
     6661 caccttcctg caccaccttg atggacggaa cggaagcatc cgcctccggc tcctgttccc
     6721 tcggtttcct cacaaatctc tggcgaataa ccttcggcct gccttctgcg ggctgctccg
     6781 gaacagaatc ggaagaatcg gaagccggct cggcgggcgc cgcatgaacg gcaatttccg
     6841 cctcagggcc ggctgaatca ccgttctccg gagcaacagc cttggcagca cgcctgcgca
     6901 ccggcttgac ggcggcttcc gttccggaga caatcgcaga gccgtcagtt tccggaacct
     6961 cctccacagg cttcttgcgc gggcgtccgc gccggggcct ggcaggaaca tcctgcgctt
     7021 cttccaccgg gatttcagca ctgctcttac gggaagtctt cttccccggc acatcgacac
     7081 tttccatccc atcatccgcc aattcagcaa ccttcttcct tatcgtccgc ttgcggggag
     7141 ccggggaaga atcctgttcc gtcccgggca cttccgcagc agtctggggc tttaccggag
     7201 cttccgccgc agacttggtt ttgcggataa tacgtttttt gggcgccggc tccggagaaa
     7261 cctgttccgg agtcaaatct gggggggtat cagacatgga aaagaaaatt gaactgcaac
     7321 aacaggcgtc tcctgaagga caacagcgtt aaccaagctg ctcaaggatt tcttccgcca
     7381 catcgaaatt ggagaagacg ttcagcgcat catcatagtc gtccagcgct tcatacaggc
     7441 gcagggcggc cttcgccgtt tccagatcat taatcacgga agcattctgc gccacggaaa
     7501 tcagcttcat ggaaattacc gtatgcccgg cttcacgcag ggcggcgcac acgttgttca
     7561 actccgttgg gctcgtaaca aacacccatt cattgtcgga atccccctgt tccacatcat
     7621 ccgcgccgca ttccagcgcc agatccattg cggcatcctc cgtaagacct tccgccatga
     7681 tgcgggcctc tcccttgcgt tcaaactggt aagccacgga accgggcgta ccgatgctgc
     7741 ctccgttttt ggtgaacagg gtacgcagct cggaagcgga acggtttgtg ttgtccgtcg
     7801 ccacttcaat caggaaagcc gtgcctgagg ggccgtagcc ttcataggta atctcctgga
     7861 tggtagctcc tcccagttcc ccggtgcctt ttttgatggc gcgttcaata ttttccttgg
     7921 gggtggacac ggccttggct ccgtcaatgg cggcgcgcag gcgcggattc agatcaggat
     7981 ctccgccgcc gcttttggcg gccagcatga tttcatgggc aaagcgggca aaaaccttgc
     8041 ccttcttggc gtcttcctta gccttaacgt acttgatctt agaccattta ttatgtcctg
     8101 acatagaatt gggaataata gtaagtgtag gtgaaaagag acaaactaac aagtggaact
     8161 gttctacaac ccggggaaca cattgtcatt tgcaaatacc acgcaggaag ggaatcaggg
     8221 tgcggccatc cgtaaatata ttacaggtac cggatactct gccggggaac acgccgcgca
     8281 ccttcaggag ctccggctac ctcttggcag tcactctcaa cacacgcaaa ataacgtatt
     8341 ccccagcatt ggcaatactt ttttaggtca cggcaaccgg ctgctttcag agttatcttc
     8401 taccgccttt aattaatttt taatatattt tgattattta aaataataaa tactgtttct
     8461 ttaatagtga aaataaaaaa atatatccgc acagcaggct cctgttacgc atccagccct
     8521 gatggtgcag ggggtgattc cgtcatgggc caaagcctga gttctaacgc cgcctctgtt
     8581 atcattgact aagaaaggga ggcgggctta gatatgccct tctttctttt tccctgtctt
     8641 atggaacaag cagcgcacca ggaaaaatca catgcggccc tttcctccgt cctgtggtcc
     8701 gcctttttaa cggtcatcaa gctctgggcc ggcattgtaa ccggcagcct cggcatcatt
     8761 tccgaagccc tgcacagcgg gctggacctg atggctgcgg ccatgacttt ttacgccgta
     8821 aaagtggcgt cccgccctgc ggacgaaagc cacccctacg ggcacgaaaa ggtggagaat
     8881 ctttccgccc tggcggaaac agccctcctt ttagttacct gcgcctggat tgtctgggag
     8941 gctgtggacc gtctgttcta caatgaggcg gagattaccc tcacctggtg ggcttttgcc
     9001 gtagtggccg tctccctgct ggtagatgtg aaccgttccg ccatgctccg ccggatagcc
     9061 aaaaagcaca aaagccaagc cctggaagcg gatgcacttc actttaccac ggatatctgg
     9121 tcttccgcag tcgtgctcct gggtcttttc tgcatctggc tggcccatct ggtccccgcg
     9181 gaatcagcct ggcacggcct cctggagagg gcggatgcca ttgcggccct gtttgtggcg
     9241 gctctggttt gttccgtggc tttcggcctt gccaaacgtt ccatccacgc cctgatggac
     9301 ggcggttctt cctccctgac gcggcaggtg ctggacgcca tgaaaaagaa tgccccggat
     9361 tatccggtca aacgcatccg cctgcgtgac ggaggctcgc gcatctttgt agagctggat
     9421 gtggaagcgc ctgcggaact gcacgtggat gacgcccatg atgtggcgga atccattgaa
     9481 ggcattgtaa agcatgaatt gccggaagcg gacgtcatcg tccatatcga accggcgcgg
     9541 gagaatcttt ccaatatgga gccggacatg attatccacc ggctggcctt gcgccaccat
     9601 gtccgcattc acggttttta tgcaggccac ggcaaacagg ccccctgtta tttcatggat
     9661 gtggaaattc cggcggattg gcccctggaa cgcggctacc atgtggtgga ggctttcagg
     9721 gattccattc tgcatgaact caagccggaa aaggtaattt gccgcattga accggattgc
     9781 cgcgacatgg aaacaggcgc cattgcggaa caggtttctc cggaggaagt gcgcctgaag
     9841 gtaaatctca ttctccagca gcacagaaac atccgcaaga tcctcaagct ggacctgacc
     9901 agggaggcca atttcccaac cttgacctgt gtgtgttccg tggacgcctc cctgactatc
     9961 cgggaatgcc accagatcgc ctcgcagctg gaaaaccaga tagaaaacgc actgcaccac
    10021 ctggggcgcg ttaccgtcat tcttaaaccg tccaagcaga acagaaattg aatgactgtt
    10081 gccggattcc cgtaaaaaca tgttccgaat ccgggaagga gaaacggttt ccattccggg
    10141 catgggaacc tccgcccgga caaagcggag ccaacgatta tgaatggccc gcaacggaaa
    10201 ctccgggcat ttccctgtcc agctccgcgc gaatgcgctg gcgctcggaa acgatctgtt
    10261 cgtcgtccgc accgccttcc cgcaggaaat attccgccat ggacagggct acttccgatt
    10321 ccgaggaaaa aacctgggaa gcgccggctt ccttcagtat atccacgtcc ctcatgtact
    10381 tggtgtgaat catcacgtca atatgagggt taagctccag agccacgcct actatctcct
    10441 tggcgtccgg aatggaagcg gagagaatca ggctttccgc atattcaatg cccgcatggc
    10501 gcagaatttc ccgctggctg gcatccccgt aaacaatatc ccctccctgc tcccgtatag
    10561 cggtaaccgt gtctatgttc atttccacga tgatgacttc caccccgttg tcattcaata
    10621 tcctcttaag aatgcgcccc gtggggccaa accctaccaa taccacgcgc cgcgcatccg
    10681 gagcgggcaa ggccatgctg ttcacgcccc ccgggtccgc aagccctatg ccgcgttttt
    10741 gcagccattt gactgcaggt ccaatctgcc ggtacaggat gggatttaag gaaatggaga
    10801 cgacagccgc cagaatgatg gcctgattgg catccggagg caggatgttg tacatgagac
    10861 ctattccggc gagaatgaaa gaaaattcac ccacttgagc cagggccacg gatacgctca
    10921 gcgccagcgg caccggccgc ctcaggcaac gaacaaccac ataagccgcc agcggcttgc
    10981 cgatcatcac cacccccagc gttgccaggg ccagaggcca gcaatccccc acggacatcg
    11041 ggtcaaacat catccccacg gaaacgaaga agagcaccgc aaaagcatcc ctcatcaaca
    11101 tggcttccgc cgccgcgcgc gcgcagaagt ccgactggcc tacaaccatc cccgccaaaa
    11161 acgcccccaa aaccatggaa gcgccaaaga attccgcgga gcacaccgcc acgcccagcg
    11221 caatgaccag cacagccagc gtaaacaagt cacgcgcgcc cgttcgggcc acgtaccgca
    11281 gcaccagcgg aatgactttt ttcccggcaa acagcgtcag ggccaccagc agggccagct
    11341 tgacgaacat ccagccaagc tcggaaagaa tgccgcccca gtctccggcg ccgctctggc
    11401 gcgcttccat cacggcgggg atgagaacca gcagcagaat ggtaaacagg tcttccacta
    11461 ccagccatcc cagggccaca tggccgccgg gcgtatggag gttctggttg tcttccagca
    11521 cccgcgtcag cactacggta ctcgccacgg aaatcgccat gccaaaaacc agtccggaaa
    11581 tcgcggacca tccgaacatc cacccgacca gcacccccag cacggtagcc acggatatct
    11641 gcacaacggc tcccggcacg gccacttttt gcacggcaat caaatccttc aaatggaaat
    11701 ggagccccac cccgaacatc agcaggataa cgcccagctc cgcgaattgt tccaccgtat
    11761 gggaatccgc aacaaatccc ggcgagtacg gccctaccac caccccggcc agcaggtagc
    11821 ccaccagcgg ggataaatgc agttttctgg caataaggcc gaaaagaagg gcagcggcca
    11881 atccgccgac aaaagtgaga ataaggctga aatcgtgatc catacgcgcg tgacagggat
    11941 aaggggtatt cttgaccgcc aagtcgtttt tacgctatcg cgttttattt tccctcacaa
    12001 gaacgcattg cgccgcatgg aaatgcagtc gcactttttg catgaaaaat ggtcccggca
    12061 aattttcatc ataccctatt tgcgaccgct tctaattaaa ttttcccata cgggattcct
    12121 atcaaatgtg ttagtaaaat tcgtcctgtt cattcccggg aagctgccgg gtcattgtcc
    12181 cgcactggcg gtcggacaaa tacccataca tcaccatcgg caatttttac atcctgctgc
    12241 cggataacgg aaataatccg ccggcccgga aaatccaaag caggaacgcc cgccgggcag
    12301 acactttcgt tctcaggctc ttccatgctc catgcccata cccctgaact tggaaaaatt
    12361 gataaaatac ccctaattct atttgaattt cccggaaatt aatttatttt atattggaaa
    12421 taattgattt tttcaaatct atgactgaag atgccatatt caaggaacag tggaaccttc
    12481 ttaagaagga gatggcaatg ggagacgccc atctccagca aaggctggaa cagttcattt
    12541 tccggcaata cggccgggcg gttctgacgc attacattaa agccctttcc gtcctggagc
    12601 tcagccgcca tttctacctg cggttccgca ggaggagccg tgccgtaacg gaacccattc
    12661 tctactggac cgtgcttgcg gaaacaacca tccacatgac gctggaggga gtaaaagaca
    12721 tgcacccctt catctccctg ccggcacgtg ccggcgcaac cttccagctt gacatgttaa
    12781 aagactgcta ccttctttat ttgagggacc ggggatatcc ctgctggccc agggcctccc
    12841 gcagaagccg catcatctgg aacaaatgga acgctccgga actggaagcc gcctatttcc
    12901 tccggttgtg ccagaacagc acggaccgcc attatgccaa cgtgatgaac atcctgctgg
    12961 aagaaatact gcatatgctg attaccctcc gccaactcag gaaccacgga aaaaacctgc
    13021 tggaactcag aagaagccgg aactccaatt cttccccggg cggcgttccg ccgccgtccc
    13081 tccgcccgca gcaccctccc gaagacagtc tttaaaacca tccgcgcttt ttccaaagga
    13141 tacttgaaaa aaccgccggg accttacaaa atcaacatgc agtctccata ggagaaaaat
    13201 ctgtaccgtt cccggatggc ttccagatag gctttcatga ccagctccct gcccgcaaag
    13261 gcgctgacaa gcatcagcag cgtgctctgc ggcaaatgga aattcgtgat cagggcgtcc
    13321 accactttgt aattgtaggg aggatagatg aagatgttcg ttgaaccgga acactccctc
    13381 aacggcagcc cttcgaccgt tgccacatgt tccagcaccc gcacgctggt ggtgcccacg
    13441 gcaataaccc ttctggcgtt attgactgcc cgggcagcgg cttcaggaag aaaaaaatgc
    13501 tccgtatgca tctgatggtc ttctatcctg tccgccttga cgggacggaa tgttcctacc
    13561 cccacgtgca gggttagaaa atcgtggggg acggatgcca gcagctccgg cgtaaaatgc
    13621 agcccggccg taggagcggc aatggcccct tcatgtctgg cgtaaacggt ctggtaacgc
    13681 tccctgtcgg atgggtcgct ttcccggttc atatagtggg gaagagccag acgcccgtat
    13741 ttttcctcat ccacttcccg gtcaaaccgg ataaggcggt acccttcctc gtcaatgcct
    13801 tccacggtgc ccaccgcctc tccgaccgca accgtacgcc caagcttcat tttgcgtccg
    13861 gggcgcacca tgcacttcca caacagggga ttaagaacct ccgcgcgtac tatttcaatg
    13921 gaaccgtcgt tggaaaaata tcttgccgga accaccctgg tatcattcag gataaaatga
    13981 tccccttccc gcacatattc cggcaaatcg caaaaatgcc tgtgctcaat catgcccgta
    14041 tcccggtgaa tgaccatcat ccgggacgcg gagcggtccg gaaggggacg gtccgctatc
    14101 agttcttccg gcagcggata atcaaaatca atggtgcgca cggccaagtg cattgccacg
    14161 aatcctccga aaggtcaata cctttaaacc gcaggtaaca caattatatc tttaccctgc
    14221 ttcggttcat atggtatgga aggacacatg aacaacctgc tgtcagcaaa catttccaac
    14281 gcaaccgcct gggggaccat tctggtcgtc gtcctcgtca tctttctggt catctttttc
    14341 gccatcatcg ccaaattctt caagacatgg ctgcgcgccc gtctggcgaa agcgcccgtc
    14401 tccatgagca acatgctggg gatgtggctc cggaaagtgc cctatcccct ggtggtggac
    14461 acccgcatta cggccgccaa ggcgggcctg gacatcagca cggatgagct ggaggcccac
    14521 ttcctggccg gcggcgacat cgtggactgc gtgcttgccc tgattgcggc ggagaaagcc
    14581 ggcattccgc tgagctacga ccgcgcctgc gccattgacc tggccgtgaa aggcacttcc
    14641 aaaaccgtgc tggaagccgt gcgcacctcc atcaatcccc gagtcatcga ctgcccgaac
    14701 cccagctccg gccaaacgcg cctgacggcg gtggcccgtg acggcattgc agtggcggtg
    14761 cgcgcccgcg taacggtgcg caccaacctg gatctcttcg tagggggcgc cacggaggaa
    14821 acggtggtcg cccgcgtcgg ggaaggcatc gtctccgccg taggttccgc cccctcctac
    14881 aaggatgttc tggaaaaacc ggaggtgatc tcccgtaccg taagcgacaa gggggtggat
    14941 gccgccacgg cgtttgaagt gctttccatt gacattgcgg atgtggatgt agccggcaat
    15001 gtgggcgccc gcctccaggc cgagcaggcg gaagcggaca aacaaatcgc ccaggccaaa
    15061 gcggaagtcc gccgcgccgc cgccgtagcc acggaacagg aaatggctgc aaaaacgcag
    15121 gaaatgcgcg ccaaggtggt ggaagcggaa gcccagattc ccatggccat ggcggaagcc
    15181 ttccgcaacg gcaatctggg nnnnnnnnnn ggtggctgac accaaaatga gagattccat
    15241 cgctcaaccg gatacgcctc cctcttccat caaataaatt ccctggtttc cggaaaggcc
    15301 tgctttttcc ggaaaccttt atcctttatg gatttactgg catacatgct cgcctccgga
    15361 ggcgatgaca atagcctaaa actctggttc tggctgattg tcggggtgat tttcctcatc
    15421 aaaaaagcaa tcgaattgtt gaaaccggat gaaaaggatg aggaaccgtc cgttccggac
    15481 acggagaacc atcatgaaaa acgcgtaagg gaagtcattg cggaaatgcg ccgggcaacc
    15541 ccgcagccac agcaaaccca acctgccgca ccggcccgcg caccgcggcc ctcagcccct
    15601 cctgtagagc ttccctcccc cgccgtggcc cgcaaaccgc agccgatccc ggcgccggcg
    15661 gctgcacaac ccgaggaaac agcccggcag acggcgcaaa atgtgctgga acgctacgta
    15721 aaagctacgg aagaagcccg gcaatccatg cgctctcttt ccgatgcgga acaggcggct
    15781 ctgcaacgtt tgcaaaagag ggagatgaag gaacatctgc ccgaccttct tccgtcatca
    15841 gccgcagtgg aatctccttc cctgaaaaat tcccttcacc accccaaatc cctgaaagcg
    15901 gctgttcttt atcaggaaat ccttggaaaa cccagagcat tacgaaacgc atccttcagc
    15961 ggtgaataaa gaatggccct tcccgggcca tgttccgttc agccgtcatg ctgcccccct
    16021 cttattcatt ctcccttgcc tgaaaaggta agcggaggta ccctccatcc cgcaatgaca
    16081 gacacacctt tgcagatagc cggacgccag ttcacctccc gcctgatggt gggaacggga
    16141 aaatttcctt ccaatgagag catgaaagaa gctctggaag cctccggcgc ccaaattgtg
    16201 acggtggctc tgcgccgggc ggatctgacg ggttcccacg atcccgccgc caatattctg
    16261 gatttcattg atccggaaaa atacctcctt ctccccaaca ccagcggagc gactacggca
    16321 gaagaagccg tacgcctggc gcgcctggcg gcagcagccg ggctgcccaa ctggatcaag
    16381 ctggagattc acccggacgc ccagtacctg atgccggacc ccattgaaac gctgaaagcg
    16441 gcgaaaatcc tggtcaggga aggcttcacc gtgcttccct acattaacgc ggaccccgtg
    16501 ctcgccaaac gccttcagga agccggagcg gcggccgtca tgcctcttgg cgcccccatc
    16561 ggcaccaaca agggagtgct gacccgggag ttgattaaca tcatcattga acaggctatt
    16621 gtccccgtgg tggtggacgc ggggctggga gcgcccagcc acgcggcgga ggctctggaa
    16681 atgggagcgg atgccgtgct cgtcaatacg gctatcgccg cctccggctc tccctccgcc
    16741 atggcggagg cgttcgccct ggcatgccgt gccggacgca tggcttatct gtccggcctt
    16801 cccgccgttt cctcccaagc tacagccacc agccccctga cttccttcct gcattaaaag
    16861 gccgggaaaa agccccggtt ttctcccgca cccatgggtc aagaaactcc gggaacattt
    16921 ttcgcaatct tcctgcgtat caggaattcc aggatgaagc aggcggctac aaacagcaca
    16981 atcaacggaa tcagataagg ctggctgaag aacatctggt aaaagaacgc ccccagaata
    17041 aacagcatca gaagaaagcc caggatgata agcagcgcat ttccctgagt ctctttccgg
    17101 agccgccaat gggcgacgaa caccgccagg tagctgatca gaaaacaggc ccccgccaaa
    17161 ttggcgatgg ctaccaggtt aaaggcattc gtcatcacca gaatccccag caaaagatag
    17221 aaaaaaccct ccgttccgtg gccccagaaa ggctgtttaa aaatagctcc cagctggccg
    17281 ttgcgcgcca gagcccggga aatgtccagc atgctgaaca tggtggcatt aatggctgat
    17341 gccgtagcaa tgagagccgc tacggatacg gccatgaagc cccacatgcc caaaaccgga
    17401 taggcggcct gcgctacggc cgtttccgta tggctttcca gggaagtagc gggaacgttg
    17461 gacagtacta cgtaggagag tatcaggtac aaggccagca caaggccaat agccataaaa
    17521 atggctcttg gcagcgtcct ggaaggattt ttcagattgc ccgccgtatt ggccatcatt
    17581 ccgtagccgg caaatgcgaa aaatgtcaat cccacgctgc ccagcaaacc gtccagccct
    17641 ttcaccggaa tgggatccgc gcccacaggc acggaagaac cgaagctggc aagcattaaa
    17701 accgtcaaaa tcaataattt gaatgccacc atgattactt ccgactttcc aacggccctg
    17761 gctcccccca tattcaatgc ccccagcagc aaaatagcca cgctggcaaa aagggccgtg
    17821 gaataaatgg tgactgccct gtccggtaaa aacaggcggg acgcataggc tccaaaggat
    17881 ttggccacca gggccacggt aatgaccagc gttcccaggt aaataagggt cagtgcgccg
    17941 gacagggtct tatgggaaaa agcctcgttg aaataatcca taattcctcc ggaaccggga
    18001 taacgggaac ccagtttggc gtaggtgtac ccggaaagca gggcagcaac gcctcccaga
    18061 ataaaggacc aatatacatt ttttcccgcc atcagggtcg cctgccccat gagggcgaaa
    18121 attcccgctc ccaccatgga ccctattcct aatgcaatga cgctccacag ggacagatat
    18181 tcttttgggg ccttcccgta cataagggat ttagagatac tacaggaatg aaacgttgac
    18241 ctttccgcac gggatgacac gtcctgctta ttcatccgtc ctgcagcccc atcatgacta
    18301 ttccggcacg gctttcccct tgaatcgtcc gttgtcggca tgtagggtac ggctgatgat
    18361 caaaaccgcc atttttgatt ttgacggaac tcttgccgac accatacccc tgtgccggga
    18421 ggcttttcgc cgcgctatcc ggaagctgga cggcagaata ttgacggatg aagaaatcga
    18481 acgtcaattc ggcccggacg atctgggagt tatccagaaa ttgattcccg ggaaaccgga
    18541 actgcatgaa cgcggcaggg aactatttat ccactattac tgcaagctgc acgcagacct
    18601 ggctcccgcc ccctttcccg gaacagccga attgctgagc actctccgca acctcggcat
    18661 tcggctggcc atggtcacag gcaaacgcct ggaaagcgca gagatatccc tccagttttt
    18721 tcatctgtcc gaattttttc ctattctgga aacaggctcc ccggagggag gcgtaaaacc
    18781 ggaccgcatc aaacgggctt tgtcccgtct gaatgccgtc gcctcagaag ccatctatat
    18841 tggagacgcc cccacagacg tggacgcatg ccgctctgtc cctatccgca ttctctccgc
    18901 aggatgggcc gcggaggcgg acatcaacgg cctcaggaaa aaaaagccgg actttctcct
    18961 gacccgtttt gaagacctgg aacgtttctt ccgggaacat catgaaaatg aaggccttta
    19021 agattgcaat cctgcagaaa acaggctacc attacccacc ccgttaatat caatccttcc
    19081 atgacttttc agaaagaatc cggaaaagag ggcctgaaaa ttctgctcat gctcgccagc
    19141 gtcattatca tcacggccgg gctgcaggcg ggaaaaccgg tgctcctgcc catcgttcta
    19201 tccggctttc tggcaatcgt cagttatccg ctgacgactt ttttcaagag ccgtctttgc
    19261 ttcccgcact ggctggcagt gactttcacg gtcatcatgg actttggcat tctggtgggc
    19321 ctgggctatc tggcccaata cctggggcag gatctggcca aaacggtcac ggtcaaatac
    19381 cagcctctta tgatggagaa aatccatgaa ctccgcgctt tcctgattga acaggactgg
    19441 aacaacctgg ctgaccagat gcttcaggaa cttccggacc tgcttaacgg ccagcgcatc
    19501 gtggcgtttt ccacaggggt gatggggcag ttagcttcca tgctgacctt caccaccctg
    19561 attctgatcc tgatgacttt cttcctgggg gaagcccccc gcttccgggc gaacatcaat
    19621 aaactggggc ataacagcga cacaggcatc cgcaaattct ccaaggccct ggccggagtt
    19681 cagaaatatc tcattattaa aaccttcatc agcgcagtta cagggcttct ggctttcctg
    19741 ctttgctatt acatgaacgt ggactttccg ctgttgtggg gcatcgtggc tttcgccctc
    19801 aacttcattc ccaccttcgg ctccatcatc gcggctattc cccccacgct tctcgccatg
    19861 cttctgatca gcccgactgc gggcatcatt gttgccggcg gctacctggt gattaacaca
    19921 gccctgggaa actgcctgga acccatgctg ttgggacggc aattcggcat tgtgaccagt
    19981 atggttctgc tctccgtcat cttctggggc tgggtatggg gccccatcgg catgctgctg
    20041 gccgtaccca ttaccatgtt gattaaactc gggctggaaa gctccaagga tctcgcctgg
    20101 atcgcccagc tcattgacaa ccctcccact cccagattcc ctctcccccc tctccattcc
    20161 gggaaaacca acgaaagcac aaccaaggaa taatccatgc attcattcgc ttacaaaaac
    20221 ggcacgctct actgtgaaaa cgtcaacctt caggaactgg cggacaagga aagcacaccc
    20281 ctgtacgttt atagcaaaca aaccatttta aaccactttc accgcctcag ggaagctctg
    20341 gcaccgctta acgcggaagt ggcctacgcc gtcaaggcct gctccaacat cgccatcctg
    20401 aacctcatgg cccgcaacgg ggcgggattc gacatcgtct ccggcggaga actcttccgc
    20461 gtcctcaaag ccgggggaga tccgtccaaa tgcacttatg ccggcgtagg aaaaaccgag
    20521 caggaaatcc gttatgccct ggcccagggc atttattgct tcaatgtgga atccgaagcg
    20581 gaactgcggg ccattaacgc cattgccgcc tccatgggag tcaaggctcc ggtggccgtg
    20641 cgcgtcaatc ccaacgtaga ggcgggaacg cacaaataca ttactaccgg caaggctgaa
    20701 aataaattcg gcgtggactt cgaacgcatt gaatctcttt atgagatggc ggcccgcgaa
    20761 cttccgaacc ttcatctgaa agggttgcaa atgcatatcg gctcccagct tacccaggcg
    20821 aaacccttct tggaagccgt caggaaagtc gcccccctgg ctgcttccct gaaagaaaaa
    20881 cacggcattg aatttttctc catcggagga ggaattggca tcgtttacca gggtacgctg
    20941 gattccggcg ttcaggaatg gtggaatgag gactgcgccc agctcacgct gagcacttac
    21001 gcccaggccg tcgtccccac cctgcaacct ctgggattac acatcattgt ggaaccgggc
    21061 cgtcttatcg taggaaatgc gggagcactc atcacgcgtt gcctgtatga aaaaaacggg
    21121 aaagccaaaa cttttaaaat tgtggatgca gggatgaacg acctcatccg ccccgccctc
    21181 taccagggct atcatgaaat tatcccggtc agagaacacc cctccggatc ctgcgtcaca
    21241 gcggatgttg tgggtcccat ctgtgaatcc ggagacttcc tcgcccaaaa cagggacatg
    21301 ccggacgtgc gccagggaga actcctggcc gtactgtccg ccggagccta tggtttttcc
    21361 atgtcttcca actacaattc acggcctatg gcggaagaag tcctggtgga cggggaccaa
    21421 tggaacgtca ttcgcagccg ccagagctgg gaagacctca tccggggaga atccattccg
    21481 gaataacacc cccatcccgc ccatctttag aatgcccttc cagcggtaaa acgaaggagg
    21541 ggcatttcct ttcaaccgta ctgaatggaa aacaaggcgg aattttcaag tccgccaagc
    21601 cacgcggagc ttctttccgg agacgccgca cgctactgcc gtacgggctc cggcggaccg
    21661 gtccggccgc aagtcacaat gattcgaaca tgaacatgtc ccgcaagagc gttgcaaccc
    21721 cggcgtgcgc ctgagttcag ccttgccctc ctgaaaaaaa acacctatca tatccccgtt
    21781 atgccaatag ccacaccaga acaatacatt accatgcttg aaacggccaa aaaggaaggc
    21841 tacgcctatc cggccgttaa cgtgaccacg atcgaagtca tcaacggcgc tctccgcgcc
    21901 ttttccgaag ccaaaagcga cggtatcatt caggtttcca tcggtggcgg caaattcgcc
    21961 tccggcagct ccgtaggcaa ttccgccctt ggcgccattg ttctggcgga agcaacgcgt
    22021 cgtctggctg aaaagcacaa cgttttcgta gcccttcaca cggaccactg ccagcccgaa
    22081 cacgtggata ctttcctgcg tcctctgatt gccgaatccc gccgccgcgt ggaacgcggt
    22141 gaagcccccc tcttcaattc ccacatgctg gacgcctcca cgcttcccat ggcggaaaac
    22201 ctcaagcttt cccgggaact gctgaaggaa tgcgccgaac tgggcatcgt tcttgaaatc
    22261 gaaatcggcg ttgtgggcgg agaagaagac ggagtggaca actccggcca tcccgccgac
    22321 aagctttaca cttctccgga agatatgctg atgacgtatg acgccctcgc gcccctcggc
    22381 aaattcatgc tggccgccac tttcggcaac gtgcacggcg tgtacaagcc cggccacgtc
    22441 aaactgaaac ccagcatcct gcgggacggt caggaagccg tggtagccaa acacggagaa
    22501 gcggctcgca tgcccctcgt cttccatggc ggttccggtt ccgaactttc cgatatccgc
    22561 gaagccgttt cctacggcgt cgtcaagatg aacatcgaca ccgacacgca atacgccttc
    22621 acccgcccga tcgtctccca catgtgcgaa cacatcaacg gagtgctcaa gattgatggc
    22681 gaagtgggca acaagaagga ttacgatccc cgttcctatc ttgccaaggg tgaaaaggga
    22741 gtcgccgccc gccttcagga agccgccgac aaccttatgt ccgccggcaa gaccctcttt
    22801 ggcaaaatct aaaaagagtt ccctcaacga atttaatgcc atgcccggtt tcttaaaaga
    22861 aaccgggcat tttttctgga aaaacccgcc aacagggtaa ggcgggaaac gctttcaccg
    22921 agtattcatc cctctgagac gacgggaaaa cgaaccgttc cccgtctttt tttctgtccc
    22981 ctgggcttct ccgattacgg gaccattaac ccgcattctt ccgcaaggaa accgctgcga
    23041 acatataaaa cgatagccgc acaggaaaaa ccagaattat tcataacaga acaaggccat
    23101 tcctccgctc agcggaccgc agggaccccg gcagcgcggc cctaccccgg cgctgcttga
    23161 accacaggag cctttcccca gcgctccagg aaccttaatt tcctgctttg cgggattccc
    23221 ttacgcggtc attggcgttc aggatgcgct tgcgcagacg gataaaatga ggcgtagcct
    23281 ccaccagttc atcaggttca atgtactcaa tggcacgttc cagagaaaaa atgacgggag
    23341 gggaaagctg gatgccgtct cccttcgtgg ctgagcggat attgttcaaa tgcttttcct
    23401 tgcacgggtt gaccggcata tccatctgcc gcgggttttc gcctacaatc tggccttcgt
    23461 aaatatcttc ctgcggcccc acaaacagac gcccgcgttc ttccagcgct accagcgcga
    23521 acggagtgga aacgcccgtc tccatggaaa ccagggtgcc ggtggtacgc gtggtgatgt
    23581 ccccggcata aggcccgtaa tcccggaaca ggtgggagaa aataccgtgg ccggacgtca
    23641 gattgaccag ctcaaattca aaaccgataa taccacgggt gggaatataa gcctgaataa
    23701 aagtatggcc cgtgccttcc ctgcatacca tgtcttccat ctgtcccttg cgggcgtgaa
    23761 gaagcttcat gacgccgttg gcatgttcgt ccggaacttc tacgtacatc gtttcatacg
    23821 gttccgtaag gcggtcgttt tcatccctct tcatgatcac ggtgggacgg gaaacgcaca
    23881 gttcataatt ttcacggcgc atagtctcca ccagcacggc gatctgcatg gcgccgcggg
    23941 cggaaacgga gaaaacgccg gccttgtccg tatcctccac ctggatggaa atattcgtcc
    24001 gcatttccct catcaggcgg tcgcgtatca cgcgggaagt cacattcttg ccgtcgcgcc
    24061 ctccgaaagg cccgttgttg atggaaaatt ccatggaaat ggtaggagga tcaatcgcca
    24121 cgaatggaag gggctccgta tccgcggaac cgcccaaggt ttccccgata ttgacgtttt
    24181 caaaccccgc cacccctacg atgttgcctg ccacgccgat ggccgaatcc gtagtgctca
    24241 ggccggaata ttcaaacatg cgggtcactt ttccggaaat gcaggagccg tcgctctgat
    24301 ggagccacac ggtgtcccct ttcttcacag tgccggaggt aatgcggccg atagccacac
    24361 ggcccacata atcgtcccag tcaatattgg aaaccagcat cttgaattca ccctccggat
    24421 cggcatcctt cggagcgggg atatgttcca caatcttgaa taaaagagga gtgcagtcca
    24481 caggaggttc cccctccatg gaattcatga agtaaccgtc gcgggcggaa ccgtacacga
    24541 aaggggcttc aaattgttca tccgtggcgc ccagctccag aaaaagctcc agcacctggt
    24601 catgcacggc ggcgggatcg gcatgcggac ggtcgatctt gttaatgacg acaatgggca
    24661 tcagcccttc ctgaagggcc ttgcggagca caaaacgcgt ctgggcctgc ggcccttcaa
    24721 aagcgtccac caccagaagc acgccatcca ccattttcat cacgcgctcc acttccccgc
    24781 cgaaatccgc gtgtccggga gtatccacaa tgttgatcgt gtatccattc cagaggatgg
    24841 atgtattctt ggccttgatc gtgatgcctt tttcacgttc cagatccatg gaatccatgg
    24901 cgcgttcctg cacggcctgg ttggcccggt aggcgcctcc cgcctgcaaa agttggtcaa
    24961 ccagggtcgt cttcccatgg tcaacgtgag cgatgatggc gagattgcgg aacttggaag
    25021 gatctgacat tgcgcgcgag agctaaaaac accggaaaat aaatgtcaag tctgctccat
    25081 gaataccgac tatattttca gcgtttgcaa gcctccctgc cgccattgcc gcaagcgatg
    25141 gaacttttat tttcctccgg tgaatttata gcttcccccc ctcctcaaat aggagtattc
    25201 tgctccggca taagtaattt ctcaattcta gataacaatg aagatttggt tagatggaaa
    25261 gctggtggaa caggaggaag caaaaacctc ggttttcgac cacggcacac tctatggaga
    25321 cggcattttt gaaggtatcc ggttctataa caagcgcgtt ttccgcctgg aagaccacat
    25381 ggaccgcctg tacaactgcg cccattatct gttgctggat attccttaca ctcaggaaga
    25441 actctccaac gccgtatgcg aaacggtcgc ggcctccggc ctaaacgacg gctatatccg
    25501 tctggtggtt acccgcggcg tcggcaatct ggggctcaac ccattcaact gcaaacggtc
    25561 ctgcgtcttc atcattgcgg ataaaatctc cctgtacgat ccggacgtat atgaaaacgg
    25621 gctggccctg attaccagct ccgtgcgccg caaccgtccg gacaccgttt gtccgcaggt
    25681 caagtcgctc aattatctca acaacatcct ggccaaaatg gaagctgtgc gccagggtgc
    25741 ggcggaagcc ctgatgctga acgacctggg taatgtggcc gaatgcacgg gcgacaacat
    25801 tttcatcgtg aaggacggta ccgtgttcac accgccagtt acggacggct gcctggacgg
    25861 catcacgcgc cgggtagtgc tggaaatctg ccgggagctg caaatcccgg ctcaggaaaa
    25921 gaccatgaac cgctttacca tcacctgtgc ggacgaatgc ttcctgaccg gcactgccgc
    25981 ggaatgcgtt cccgtgacca aattggacgg ctatcagctg ggctctggaa aaatcggtcc
    26041 tgtcacagct cgaatcctgg cccgtttcca ggaactggcc cagaccacgg gaacatcctg
    26101 ttagaaccag ccaacccttt tttggaccaa cgaaaacggc tccgccagat gacggagccg
    26161 tttttttgtg ctattccgtt aaatataaaa aagaaaacac aggtaagcac attcccgcag
    26221 ggctatacgg cagtctcctc tggaaatgaa ggcttgtgac cttcacggcc tcccgttccg
    26281 aaacgctgca gaagaatcca atatgaaaag ccgcgccgct tgtgaaatga caaggcaagc
    26341 gacatcttgc atgtccggcc gccacgccgg acatgcctca acctgaaaaa aggctatttc
    26401 ttcttgggca ggccggtttc cgggtcaaac tgcttagcct gctgggcatg ttctttcgtc
    26461 tgatagaacc gaacgtaatc aatcaggaat tttacaggat acccttgccc gtctttagga
    26521 gctttctcgc accaggtgcc gcccacggcg gaattgagaa tgagatagaa cggcgtacgg
    26581 aacggattcc ccgccccatt ggcataatta gtggtattaa ggtcaatgga cttcaccagc
    26641 ttgtcatcca ccatcaactt gatggaatcc ttgtcccatt ccatgacata ggtatgaaaa
    26701 tcatcggaaa gattctctat cttcacagtc cctccgcggg aagcgtccct tgtggacacg
    26761 ccgtccggac tcaagtggaa agtggaataa accacatccg gctgttggga aatattctcc
    26821 agcatatcaa tctccccgtt gacgggccag ccccatttat tcttgccaag caaccagatg
    26881 gcaggccaga tgcccttggt tttgggaacc ttggcacgca cttccagtct gccgaacatg
    26941 aaattttttg ttttgatcgt cgtaacggaa ccggaggtat agggcatgaa cttggtattc
    27001 ttgatccaat ccgcggaact tttcttataa ttgacattgg cgaacttttc aaaatgggtt
    27061 tccaaaacca ggcagccatc ctccaatctc atattcttgg gtcggcctgt ataagtctgc
    27121 tgggagccct ggttacggat tactcccaac tccggtttcc actttttggc gtctatcctg
    27181 gaaccgttga actcgtcacc ccagacataa acccacccgc cggggagggt cctcggaggt
    27241 ttggaatctt ctccctttac agacatgcaa aggcatgcag tcaacaacac ggcaaatagg
    27301 ctcttcatac ggagcaatac tgcacctagt tccgcagcct ttcaaacaaa atacatcatt
    27361 cgggaccaca tacctccttt cccctgtcaa atggcgaaat atcctgcaaa atgaatagaa
    27421 cccttaaccc ggcgcatgct atggtcatga tatgaaaacc actttccctc cccatggccg
    27481 ggcgctcctc caccagacgc ctgcaggtgc tgtcgtgatt gtggaaaacg gcacagccgt
    27541 cacccatctt ttcttcagcc gcatggcaca gccggagcat ttggaatggg aggaaacacc
    27601 tctgctgcgt caaacggctg accagctgga tgaatatttt caaggttccc gccgcgtatt
    27661 tgacgtgccg ctttctcccc aaggaaccga atttgaacga acggtctgga aagcccttca
    27721 agccatcccc tatggagaaa ccagaagtta cggagacatt gcccggcgaa taggacgccc
    27781 atcagcatgc cgggcagtag gacaagccaa caaccggaac cctgtcggca tcatcatccc
    27841 ctgccaccga gtcatcggag ccgacggcaa actgaccggc tatgcaagcg gcctgaccat
    27901 taaacaatac ctgttggaat tggaacaggg ctccgccact cctttcacat tattcacgga
    27961 tgtggaacgc atgcccggaa atactccggc cagtccataa aacggcagga ctggaaaatt
    28021 ccggtcagag ctgcataccg ttttccgtct gaggaagagg catggatttc aaggcggcct
    28081 tgggggattc aatacccagc cattgaagag gcagcaattc acccgttacc ctgtaatgtt
    28141 tgtaggttaa tccggcaaac acagccagaa aaaatatcag gacggcataa agggccatcc
    28201 ataaataatg attccgggtc gtgcgggcgg cttcatccgc caactgctgt aagttccgca
    28261 tagcgatcga aggagcggcc tcttcttctc tcccggataa ttcggcagaa aatcccggag
    28321 atggctcttc cgcaaaccgg agcctggcat cccccaaagc gatttcatcg ccatcgtaaa
    28381 gggttgcggc gcccatcggc gtcaggccgt tgattttagt tccattagta gaaccaaggt
    28441 cctccaggac atatccccct tccttaaact gaagcaaggc atgctctccg gaaaccgact
    28501 gaaagggaag cacaatctcg caatgctcgt cacgtccgat gcgtgcgaaa tattcaccgt
    28561 tcttcggaag aactatggtt ttttcagaac cgtcgggaag gtggatggaa aggcgtggca
    28621 tgattcctgg aaatcatgcc acgcccatat tcattttcaa tccaaaagac cgtttgccca
    28681 tcacgtataa gaaaaatggc gatatccgcc tttccccaga aagacagaat tccatgcaga
    28741 ttcatgatat gggttcatcc tcctcaattt ttatttttcc cgcatacttt caaaaaatat
    28801 tttaagcaat tatagtggcc tccggggaag ttttccataa gtaatccact cattttaaaa
    28861 cattacatga aatactgaat caattttttt cctgattgga aacagacctt ccgaaactgc
    28921 gacggatcat gccggacacg aacactgcgc aaacgacaat cttcaagggg gacaacttgt
    28981 tgggaatcgc taaacaggcc attttcaaac ataccgtttt ccgggagggc cgccctcccg
    29041 gaacataggg attggatata cgcgcaaact gccgtcctgc ctttggacat gtccttcact
    29101 atctcgataa tttttttatt tgcctggagc ggcttaaatc aaaagaactc aactgaaatt
    29161 ttttctgctg tctggcatct gccgctcctc ttctggcatg gagtttattc accctgggtt
    29221 cccttccccg tccgttacat gcagacgtct gcatgacacc aataaaagcg gcatgtcgct
    29281 gccgctgtcc ctgattattt taataagagg aatttttctc ttcatttatt actgtgaaga
    29341 cagccagcat ggcacaaatc aatccggggc atcgtaaaaa catccggagt tcccgtaatg
    29401 attcgaaatc ctctttcacg ggaaagtcag cggcctacac cgtggaaaaa ccgtgcagcc
    29461 ctgcacatgt cttgcgtttt gaaggaaaat gtttcatatt cagaccatgt ttgttgacaa
    29521 catccgcata ttcgccaggg caggaaaggg aggaaacggg ctggtcagct tccgaagggc
    29581 caaattcgtg cccaagggag gcccggacgg cggtgatgga ggcgatggcg gaagcgtcat
    29641 tctggaagtg gatccgcaca cgaatgacct gcgttctttt ttttatgatc caaaattaat
    29701 cgccacggac ggggtaggcg gacaaagcnn nnnnnnnnac aaagcgccaa gaaacatggc
    29761 aagaacggca aatccgtcat cgggaaagtg ccccccggta ccattatcta ccgcagcaat
    29821 gcatcctcca tggcggaggc gacctggctg gaacgggaag gcgaaggcat tgaactggaa
    29881 aaaatagcgg atctgacgga aatcggcacc cggttcacgt tgtgccaggg cggcctcggc
    29941 ggcaagggaa actggcattt ccgttccgcg accaaccagg cgcctacgga agccgagatg
    30001 ggaacggaag gggaggaagg cgtcttcttt atggaattgc gccgcattgc ggacgccggg
    30061 ctggtaggct accccaacgc ggggaaaagc acattgctgg gagacatttc ggaagccaag
    30121 cccaaagtag ccagctatcc ttttaccacg cttcagccta tcatcggagt ggtggaattc
    30181 gacagctttc gccgctgcgt tgtggcggac attcccggca tcattgaagg cgcacacaac
    30241 aaccggggcc tgggacatga attcctgcgc cacatcacgc gctgcaaggt gctcgtcttc
    30301 gttctggaca tggctggcag cgaaggccgc gaccccattg aagaccttca aaatctccgc
    30361 acggaaatta aactgtacag cgaagacctg gccaaacagc cctggttcgt cgtcgccaac
    30421 aaaatggacc tggaaggagc ggaagaaaat ctggctaact tccgcatgcg tttcccgaaa
    30481 gtggacatca tccccatttc cgcgctcaat ggagacggta tttcccggct caggaacagg
    30541 cttgatgagc ttgtaggtta taaattcatc cgttaactta ttcattcaac tttctttcaa
    30601 cctttgacat gacagcccct ctctccgtaa cgacagcttc cgaccagctt ctgtgccgta
    30661 tcgccggaat tggagacctc ttcgccattg aaggggaatt cgtcaccggg aaggaaatac
    30721 ccagcggaca catcaacacc acttacaagg ctacctaccg taaaagcgac gggacggaag
    30781 attcctatat cctccagcgc attaacgatt acgtcttcaa agaccccaaa gccgtcatgc
    30841 gcaacgtgga aaaagtcacg cgccacatca actggaaagt cctgcgccgc ctgaaggatt
    30901 ccgccggcca aaccctcaac ctctacccgg cccggggagg acgcaactac attgacatcc
    30961 ccggtgacgg catctggcgc tgttacaact acctggccgg cacgcacacc tacgacgtgg
    31021 tggaaaacac ccgccaggcc taccaggcag gatttgcctt tgggtccttc caggatttga
    31081 ttagtgacat gaatccggac gacatcgtgg aaaccatacc tggcttccat catacccgga
    31141 accgcttcaa ccgcctgatg gaagtggcgg agctggatcc gcaggggcgt ctctccacct
    31201 gtctgcctga attggaattc attaaagccc gagaacagga tgtggatcgg ttgctgaatc
    31261 ttcaggaaag aggtgttctt cccacccgca tcactcataa cgacaccaaa atcaacaatg
    31321 tcatgctgga tgaggacacc gaccacgccg tctgcgtcat tgacctggat acggtcatgc
    31381 ccggccttgt actgtacgac ttcggagata tggtgcgcac cgtcactccg cctacggaag
    31441 aagatgaaga agacctggac aaggtgcgca tgcgcatgcc catgttccaa tctatcgtag
    31501 aagggtatct gaccgctgcc cacggcttcc tgacccaggc ggaaatagac cagcttgcct
    31561 tttcaggaaa actcatcacg ctggaaatcg gcatccgttt cctgacggac tatctggaag
    31621 gcgacccata cttcaaggtc tcacggccga accacaatct catccgctgc cgtacgcagc
    31681 taaaactggt ggaatgcatt gaacaggaac ttcctgccat ggaacaatac gtcaagcgcg
    31741 tcgccagggg gatgtccaga aaatagggaa aagcttttcc ttcttccatt ccactacctc
    31801 cggggacggc tatgccgtcc cttctttttt atattatttg aacatggctg cccattccat
    31861 aatccaagaa acattacacg caatagccaa gccatgtttg aaataccaga catccaactc
    31921 aatcaatatg gcaagccggc aaggatcatg ttcctggctc catatgcccc agacgctccc
    31981 gatttctcca aaaaacccta cacaggcaat ggaggctacc ctcagtacca ctacaacatt
    32041 tataaagcca tccaggacat aggctacgac gtcgtctcct cctccaaacc ctattccgtc
    32101 caattcgcca agggaaacgt ggactatgta ttctccctga tgaacaggtt cgccatgtcc
    32161 aggcctgaaa tattcatttc ttcttactgc gaattcattc aggttcccta cctgggcgcc
    32221 ccgcccaaca tccgcgccat agcggaagac aaatttctga ccaaaatggt attccgctcc
    32281 ctggaattga acgttccgga aggcattgga ctttccggag aaaaaggtat tccggcccag
    32341 gcccccttcc ctggccccta ttttgtcaaa aagcgttttg gagccgcttc cggcggcatc
    32401 cgggaagaca gcatctgcag ctcctggaaa gccgtgggtt catgcgcccg gcgcctgatg
    32461 gaagaagggc acgaagtgct cgtggaacaa tactgtgaag gcattgacgt caccgttccc
    32521 gtactgggtg gggaaaaccc cgtcatcctg ggatatgtgc agccgaaatc cgacaaacca
    32581 ggcagcatca tcacggaaga cctgaaactg cacgaccatc tgggttatca gctggtctcc
    32641 gttcaggata tggagcagga catcgccagt gacgtccgga aaatctggag cgctctgggg
    32701 ccgatggact atttccgcat cgactataga atcgacttcg agacgggaga acggcgcatt
    32761 ctggaaatga acatctgctg ccacctgggc aaaagcggct ccatctgcct ggcggccgca
    32821 gaacacggct actcccagga ggacctcctc aaatacatta tcgcctacag catggacagg
    32881 cagaaaaacc tccgccaata cggcacatgg ctcatctgac ccgcaggaaa gaagcggttc
    32941 gcattccggt cagaacaaaa acggcagtta ggaatcaggt atccggctga ttgcctcaaa
    33001 cacctcctgc cagctttcca tggaagcttc ctgcccgccc agggccatgc cccgctcacg
    33061 ggcggcagcg gccatgtctt cccggtagtg gggctcccaa gccattttag tggcgtacgt
    33121 gatgaaatcc ccggggcatt cgcacagcca tccggtcttc ccgtgttcca ccatttgttc
    33181 ccaccctccg cgcctgtcca caacaagcac actcccgctt gccatggcct caaaacccac
    33241 acgcggccaa ttctccgtcg tatcggtagg ctgcaaaacg atgtggcagc gtcggtaaaa
    33301 ctcctgttgg gacaaagact tttgatcatg gaaagtctcc acccaatcgg gaggcctgcc
    33361 agtcttcacc tcgctccgct ggtcaaaacc gagaaaagtt cccctcttcc ggaccggaga
    33421 agcaaaatgc tcgtaaatat gccacgtatc cttgctgaac ttatcttcat cctgccggga
    33481 aatgtgtccg gctccaaacc agtccgtgga acgccctgca ataaaaggaa aatctgccgc
    33541 atcaaaatac ggcctaaacg tcatgaaccg gatggccggg tcgggattta aagccctcag
    33601 gcgaggaatg acgtttccca ttacgttgct attttgataa agaaacaggg aaatatctcc
    33661 acgcttcatg gcttcctttt ccctgccgaa aagccaggtc atgcaattga cgaaaactgt
    33721 ccttctcgtc cgttttcgta tcttcgggag agcagccaga aactcttcat tacaaaaact
    33781 gatgacggga gcccctgccg gaatggcctc ccaatcatag ccttcatgca ccgtaacacc
    33841 caaattcatc atctccggat aaagcccggc acagtatgca tttttctggg tg
//
LOCUS       DAWEMX010000010        32541 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_562_length_32541_cov_12.764914, whole genome shotgun sequence.
ACCESSION   DAWEMX010000010 DAWEMX010000000
VERSION     DAWEMX010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 32541)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 32541)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32541
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_32541_cov_12.764914"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    26811..26820
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    32015..32036
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7631 a         7955 c         9230 g         7693 t
ORIGIN      
        1 tgttctaatt atttgttaga aagataaaaa atgagaatgt aataaacaca atatatgtaa
       61 cttataaaaa tatgtttcat gcttagcaaa aatattttaa cttataaaaa tattcaataa
      121 tcgataggct atggctacac tctcttttga tactgcaatt tacactcctt cctccctaat
      181 tgctacagtt cgagagttta gaggtatttt aaaactcgaa ttgaagaaaa atataacgac
      241 tggtttactc gagctccatt ataatgaaga ttcaacaaca ccagacaata tttctgaaac
      301 tattatacaa gctgtaagtg ataatgtatt gcgagaaaat cttgatgcaa actttagatt
      361 aatgcgcgag caaattgtga attgtgcttt ttctgcaatt cggacgaaat gaagctattt
      421 ttctgatatg aatactattc ttctcccatt tgagttctct cgggagtctg gctatgtttt
      481 actttctaac gagagtggag atttcatcca tttaactgaa aaagattttg atcatttaat
      541 taagcatgaa ttttcacaaa tatcagaaac agcttttcat accctttata gtcgacattt
      601 tgtctgtgat aaacaagacg ttgattttac catcaactta atagctaata aatatcgaac
      661 gagaaaagca tttctgcgcg attttactgt ccttcacatg atggtaatta cgctcaaatg
      721 caaccattcg tgtcaatatt gtcaggtttc aagtgcaaat gcggatgcta ttaaatatga
      781 tatgagctca gaaacagctc gcaaggttgt tgagatgatt ctccaagctc catcaaggag
      841 tatcaagatt gaatttcagg gaggagaacc acttctgaat tggtctgtta tcatgcaaac
      901 agtcgaatat gcggagaaat tgaatgaggc atatcaaaag aatatctctt ttgtgatttg
      961 ctccaacata tacgcattga accaggatca tcttgattat tttaaagctc acaatattca
     1021 tatttcaaca tctttagatg gtcctaaaat tttacatgat actcatcgtc ctatgcgtgt
     1081 aaaaaggctc agcagttatg ataaatttat agaaaatctt gattgggccc gcaaagagct
     1141 gggaagagat aaagtatctg cattgatgac tacttgtagt gactctctat cccagattaa
     1201 aagcattgtc gatgaatatg tcaaattggg attcgaaggt ttgtttattc gtagtcttaa
     1261 tccatacggg gatgctataa aaaataggtt atattactct ccagaaagtt tttttgaaat
     1321 gtacaaaacc ggattagaat acattgtgga gctaaaccaa aaaggtgtat ttttcgtcga
     1381 atatctgaca gagctattat ggaaacgtat aatgactcca tatccgacag gttttgtaga
     1441 tttacaatca ccttcagggg ccgggataag tggtgtaatt tatgattata atggtgatgt
     1501 atatccggct gatgaaggtc gaatgcttgc cagaatgggg aatacacatt ttaaattagg
     1561 aaatgtctat agtaacagtt atgattctat ttttgacgga gaacttatgc attccatcgt
     1621 taaagattct tgtatagaaa caattcctgg atgcagtgat tgtgtgtatc gtccatactg
     1681 cggtgttgat gtgttccgca actatttaga gaatggaaga gttgatgctg tcgctccgga
     1741 tagtttcttt tgtaaaaagc aaaaactcat ttttcgttat ctttttgaaa aaatgaatga
     1801 tgggaatttt gtaaagattc tgaattcttg gattcattga aattatgaaa aatgtaaatc
     1861 tgcatttgtc aaaatcccac atacgtagtg atgagatttt aagggtgaca aaacatttga
     1921 ggttttctcg taataatgca ctgttcgtag gaaaaccatt attgagatgg ggatatcgag
     1981 caataactac caataccaac gaacttggtg aaggagatat tgtaaaatta tatccggatg
     2041 gattatgtaa agtgctgtgg agtagaggat caattcataa cgtgcttttt actactcctg
     2101 catgtaatgt taaatgtctc atgtgtcccc aacctccgtg ctcagacaat gtgcaagtac
     2161 ataaagataa tttgaaaatt ctttctttga ttgactatga tccaaaagta atttgtattt
     2221 caggaggtga gccgtttttg ttccctgatc gggttaaagc ttattttgaa ataataaacc
     2281 gtagatggcc ttcttgccaa gttgatgttc ttacaaatgc aactcgcttg tctaaatttt
     2341 ctttatgtaa agaaatagct ttagcatcac ctataaatac aactttttgc gtatctttac
     2401 actcagatgt agcaaaagta caacaaaaaa ttaatcgtgt aggaactagt tttaaagata
     2461 ctgtttgtgg tattaataac ttggggaaac ttagacaaat gatagaaatt agaccagtta
     2521 tcacaaaggc taattatagg tatctgtatg atttcgctgt ttttgtcgtt cgaaatttcc
     2581 cttatgtttc ccatgttgca ttcatgggtc aagaaattgt aggatgtgca cggaataatt
     2641 atgacaaaat ttgggtggat cctacagatt atattgaaaa tttacatcaa gctgtagaat
     2701 atttagacca aatgcgaatt tgtgtttcta tttataatat tcctctttgc ttattaccca
     2761 aaaatcttca tcgattcgca acacgttcga tttcagactg gaagcagggt tataccccag
     2821 tctgttccgg ctgcaagcta cgcgatgaat gctgtggagt tttccttacg agtggtcaat
     2881 acctttctcg gggaatttct ccggtaacga aataaaataa aacatataaa taaaacaatg
     2941 aagtttaaaa cactaattgc acctatcata gcactagtta cttctactgg aactactcat
     3001 ggaactataa ctccatatca ggagttagaa gagcgaaata cattaattgc ttctatagac
     3061 gaagacacac cgctctattt tgatcaggct ggagctgcga aagatagtga gtgttcacaa
     3121 tatggacatt cttcccattc ctctcactct tcgcacagtt cccacagttc ccacagttca
     3181 caccgttcgg gctggtaaga attacgaaga aatagtacct aggcattttc ctaattagga
     3241 aaatgccttt ttgtgttata ttggatatta tataagttat ctattataat taacaaaaat
     3301 acctttgata aaaattacaa ggaaatttct ttgtctaaat attttattgg atataagtat
     3361 ctgaattgtt tgaagatttc tcagcaggac tttaatagtg atatcatatg ttttttgaga
     3421 ttttgttata ataaatacta tcgctccaat aaataaattt ttactaatta accttttaaa
     3481 ttttgtgtac aaattaataa gctatatctg ttccagtcca tagcaaaacg attgcttcca
     3541 cttaggtaat gctggatgat atcctatttg tctgtgggat ttttttgaaa tgttgtgatc
     3601 aagtttagta ttatttcaaa aaggttggga gattaaaacg ctgggttcag gggaacaaga
     3661 cgttttgacg acgctgtctt ttgagggagt gcttttttct tattgtcgga atacttttct
     3721 gttgatgcat tcctctttcc tgttcccttg attctttcct ttccggaaaa aggcattatg
     3781 cggaaggtgc gtgcagggaa acggattggc cggggtatat ttccggaatg acggaaatgc
     3841 tcttgatatt ttttccggct gtcagggagc ggagcatccg tatgagcttg tgaaccgttt
     3901 ttgtctggtt ctcttcataa taggtgatcg tgggcagcag gcgttccatg aagggttcat
     3961 acgtcaggct gaccaggctg agctgatccg gaatgctcag cgaacgggct tccacccagg
     4021 aaaggatggg aacgatctgg ttgccccgtg tggctaccag ggcggacata tccggatgtt
     4081 ctttaaacgc tttggccagg tattcaaaga agagaggcgt ttcaaacggg gtgggaacca
     4141 ggatgggatt ccatccctgt tccgcaaagc tgaaaaaacc tttttgcatg agctggtggc
     4201 ccttcaacgg atcaggaggc aggcacaggc ctaccgttcg gtgccccttg ttccaaaggt
     4261 gctgcgccgc atgatgcgtg gtggccgccc agtggcggtc cagataggga aggttgctgc
     4321 tctggtagga ggtgccgcgc acaatgcagg ggatgcggtg cgtcttgaac cagagctggg
     4381 tttgctccga ggaacggtga agtatccagc agacgcattc ggatttggta acaagcttgg
     4441 caagcctctt gtccgggtta ttgcctaaga tccagggagc ttcaaagata cggacgctga
     4501 tgccgtcttc ctccagaatt ttggaaatgg tatatatctc cgccaatacg ctttgagcca
     4561 gccttttgag gggctggggc gtcagaaatc cgagaatttc ttttttttcc tgaaccggat
     4621 ggcagaagat atttttaacc gtttcttctt ccgtagtttt caggatgcgc ctcggaacct
     4681 tgatgcgtgg gggggcaatc catgcatccc tttccagaag ggtcagggcc tttctgatgg
     4741 tttccctgcc tacggataac ctgtgcccga gttctctttc tccgggcagg atattgacca
     4801 ggctaccgct gagaatcatc tgtttgattt tattggctac tgtttcaacc aatgaagaag
     4861 ctggcaacga agtcatactc ttgtatatac gtatcgcatt gcgtgaattc gagccaattg
     4921 tagggattgc ctgccccgca gaatccaaaa ggagagctgg tgttgttgca agttgttgat
     4981 attctgtaaa tcgttttagg atttaagcta ctggtctttt ttgatgaggt aaagtgggcg
     5041 gataatgtat aaatgatatg gtgttatcaa tgcattacat tcctgagcgg tacggctgca
     5101 ggtggtttgt aattatttct taattcattt aagataaata ttttattgcg tattttgttg
     5161 ggcggagcca tttcttattc tccattttct cactttcccc caatttccgc caggaaatgt
     5221 gcacgtatcg gcatggatga cttttctccc gtttccggca ctaaatctgc ttgatgaaag
     5281 tgaataccat accgggcgca tcctatgcgc tgacttgtac ggctgcgtgt actatccatg
     5341 cgcttcttag cgacgcttct tctctggtta tcctggaaac ggaaaacccc ggacaatatt
     5401 tttttatagc tcccactgat acaatagagg tatcagacga acacgccctg gtgacacaaa
     5461 cttttaaagc agccgctccg ggattgtctg cccgcagcgg cataaggccg ggagtaaatg
     5521 ctgatttaaa aaatccgacg gtggaaaaag cggtatgaac catttctttt ttctgattgc
     5581 ccgcgattaa gcgcagcgct catgagcaat ccggtcaaaa ggttgtttgt ccatggtttt
     5641 tcgaaccttt acggggagcg ggacttcacc gccagatcat tgtctgacgc ggactgggtg
     5701 cggaagtccg tctcattcct tcctgaaatt actacgggga agtctctttg agaaatgaag
     5761 cgttatcgac tgataataga aaaccctggg agcgtttagc tcccagggtt tattgattgg
     5821 taccgccggt gggactcgaa cccacactct tttggaactc gattttgagt cgagcgcgtc
     5881 taccattccg ccacgacggc acgtgaagcg aaagaattaa gaggcaagag ggctggagag
     5941 tcaagaattt cctgtggaat atggtgagct gctgaaaaaa cggagggatt ttcctgcgga
     6001 ttccctccgt catgtttccg gatggaaata agtgtttgga atgagcagtg cacggccgcg
     6061 ttaacgcttt tttgcgtctc ttctcacgcc gccaactccc aaaatgctcg cacctcgcat
     6121 cctcgaatcc cctgcgtatg cacatagcgc gtctgcaaca gcgtcccgtc ccgatgcccc
     6181 atcgccgcct gcaaacgccc ccaatcgtga aacatcttcg catgatacga cgcaaaggta
     6241 tgccgcagga cgtcctgacg ccatgtctga aaacccgctg ccgagcgcaa agcgcgccac
     6301 cgcctgttcc aatctggcgg acagatgaaa accgactccc tctctgcccc ctgcggataa
     6361 taactcctca accatgccaa cacggagcga gacagcggca cctgccgacc gccgcccgtc
     6421 ttgctctgcc tcgggcgaat ccgcacctcc ccctcctccc aatccacatc gccccaggtc
     6481 aaacgccgca cctcatacgg acgaatcccg ccgtagagca tcatcaccac gcccgccgca
     6541 cagggggaaa actcctccct ctctgccgtc cgcagcaggc ggcgcacttc ctcgatacgg
     6601 agggggacga tctcccgctc cgtcacgcgc ggcacatcca ccagccgcac gggattatgc
     6661 gcacaccagc ctttcttcca cgccaccgtg aacactcccg agagaatcgc ccgcgcatga
     6721 cgacgttgcg agggtgaaag aaagactttc tccaacacag ccgcacagtc cgccgtcgtc
     6781 atcgagcgca tgggacgcgc cgccagatcg ggcacatctc gcatcatcct ctgcgtgaag
     6841 gagcggatat cgcgcagggt gagcgccgaa cggtcggcct tgacgcgcag ggtttctgcc
     6901 acggctttgg agaaggagac cgtttgcgtg gcgagatgct cggcttcgca ccccaaggcg
     6961 atgacgcggc ggcagtgaag gagcagagct tctttctttc ttacggaaga gcctccgctt
     7021 ccttcgacca gctccagcat gagacgagca acatcggtga gggagaaggg gagagaggcg
     7081 aggagggcaa gggcggtgct agcgtcggtg ttcatggcaa caggataaat aatactcttc
     7141 atcaagccca aaataacgaa acttgtcaaa aacatggaat ggaacggtag ttatgcctgt
     7201 atgacaagaa aaaaatgaga agaattttct tccttttttc ttgtttcttt gtctggctca
     7261 ataatataat atattgagaa tcaaaacatt gaagttaaga gcattattta tactctactt
     7321 ccctcgatga aacatcgagg gaatgaggct tcgggagaga cgcaactcga tgcctccctt
     7381 gtggctattc tcctcgccga ccgttcgagc ggcgctttca tccgctgggc ctgcaacgcc
     7441 tacactaccc acggcgagtc ctatgcggcg gatcaagaga tctaccccca tcaggtgcat
     7501 ctgattcagg agcgcacccg caagacgcag gaagaacagc gcgaccgcac caaaaaatcc
     7561 gccgaagtct ttacccctgc ttggctgtgc aacgccatga tcaacgcccg cgatgccgtt
     7621 tacttcgggc gggaggaggt ctttaaccgc atggaggctc catcgtggac gccgacgcgc
     7681 aagacgattg actttccgac gacagcatct ggccgccgtc tcgcgtggga gcgttacatc
     7741 gatgcccgct gtctggaaat cacctgcggc gaagccccct ttctcgtctc gcgctacgat
     7801 gccgtcgatg gtcgccccat ctccttggca gagcgcatcg gcatcctcga ccgcaagctg
     7861 cgtatcatcg gcgagcatac ctgcaccgca gaggactggt ttcactgggc aaaacgcgcc
     7921 ctcgaaagcg cctacgctta cgaataccag ggcgacagcc tctttctcgc ccgtctcaat
     7981 ctttttctga gcattagcga gtaccaccgt cacctgtgga aacgccccct caaccgacac
     8041 caacaagagg aagttgcccg catcctctcg tggaatctct ggcagatgga cggcctgacg
     8101 gcgacgactc cctttgccac ggaacagggg aagcccgagg attccttatt tgatttttac
     8161 gccatcacag ccgaaaggcg ccccctccgt agcctcatcc gcgactggcg cggcaaaaaa
     8221 acgattcgat tctctgaact caacctatcc accaccatga aatttgattt tgtcatcggc
     8281 aatccgccgt atcagctgga aaccgccaat aaatcccttt ccaacggaca attgccaagt
     8341 aaaagcattt ttcatcattt tcagctgagc gcggatcaga tttcctccgg tcttaccgtc
     8401 ttgatttatc ctggagggcg atggattcag cgttctggaa aaggaatggc ggatttcggc
     8461 ttacaacaaa tcaatgacag ccgtttacaa accttgtatt attaccccga cagtaccgat
     8521 ctcttccctg cacaagttgc cgaaattgcg gacggcatct ccattgtggt caaaaatgcc
     8581 cacaagacga ccccaagctt ccgctacttc tatatgcggc gcggagaaaa aacaggtgtc
     8641 gagctggagc ctcccggcga aaatatcctt cctctagacc cacgcgacgg agccgttgtc
     8701 aggaaaatcg aggattttgt tcaaagaaac aagttgcctt atttgaacga taatgtgcac
     8761 tcaagaaatc tatttggaat tgagagtaat tttgtcgaaa aaaatcgaga tcaggttcgc
     8821 ctctatcaag aaggggatgc ggtagattgc gagacggaga tcaagctata tgctaatgac
     8881 cgagccggga aagctggcag aacgacatgg tttgttgcac caagaagcat tattcagacg
     8941 aatgaggctt acatctccaa atggaaggtt gttgtttcca gtgcaaacgc cgggggacaa
     9001 aagagagatt ggcagttgga aatcatcgac aatcaatcgg cattcggtcg ttcgagggtt
     9061 gctctttctt cttttgaaac gaagcaggaa gcggagaatt tttaccacta tgtgaagagc
     9121 tatattattc gctatgcctt cctgatgacg gatgaagctc tgacaacttt ggcgctgaaa
     9181 gtgccggata tgtcagatta tacttctgac aacaagttaa tcgactggtc gcaggatatt
     9241 gatagtcagt tgcagaagct catgtccctc agtgatgctg agtttgaata catcaaaaac
     9301 acggtgcaga gtgtacgagc ctaaaaggag aatccatcca tgaacaccat caacatccga
     9361 agcacgaaga aggttgagcc gaagtgttac gcctacacga ctccagaggt gcagcggcac
     9421 gagggctgga cgaagattgg ctacaccgag caggatgatg tcgaggtgcg catccgccag
     9481 cagacgcaga cggtggatgt gcgctattgc ctggaatggc acgagtcggc gcgttttgcc
     9541 gacgggacgg tgtttcacga taaggcgttt catcgttact tgggcaagaa ggaggtggaa
     9601 cgcgagggga attcggagtg gtttcatctt gtgcccgagg tggcgcatgg tcattttcgg
     9661 gagtttaccc gcaatcgcgg ggtgttgcag acgccgggtg cggtgcctta tgtgttgcgg
     9721 gcggagcagg cggaggcggt cgagcggacg cggctctata tggcgagcca cgcggggcaa
     9781 agcccggagt ttttgtggaa tgccaagccg cgtttcggca agactttggc ggcgtatgat
     9841 ttgtgcaagc agatgggtgc gcggacggtg ttgattgtga cgaatcgtcc ggcgattgct
     9901 cattcgtggt acgatgatta tgtgcgtttt atggggacgg agtcggggta ttatttcatc
     9961 agtggggcgg atgctctgcg gggcttgccc catgtgctgg agcgcgcgca gttgccgagt
    10021 gatgccgagg ggtatatcga gtttgtgtct ctgcaggatt tgaagggttc tctgtatttc
    10081 ggcggggagt tcgataagtt ggagcatgtg caaaagttga cctgggatct gctgattgtg
    10141 gatgaggcgc acgagggggt ggatacgtac agggcggatg tggcgttcga gcagattgcg
    10201 cgtcgggcga cgctgcatct gtcggggacg ccttttaagg ctttggcaaa tgaaaagttc
    10261 gaggagaagg cgattttcaa ctggacctat gccgatgaac agcgcgccaa gagggagtgg
    10321 caggaggagg cggaggagga aaatccctat gccgccttgc cgcagttgga cctttatacg
    10381 taccggatgt ctgaggtagt gcgcgatcgc ctgaagcagg gggctgattt tgacgatgac
    10441 ggcgaaaatg aggcctatgc ttttgacttg aatgagtttt ttgccacgaa gagtgacggt
    10501 tcgtttaagt atgatgaggc ggtggagagg tttttggagg ctttggcagg gcaggagaag
    10561 tttccctttt cgacggaggc tttgcgccgc gaggtgaagc atagtttctg gctgttggac
    10621 cgcgtggaca gtgccaaggc gttggcgaag aagttgaagg cgcatcccgt cttccgcgag
    10681 tacgaggtgg tggtggcggc tggcgatggc aagacggatg cggaggagga ggcgacatcg
    10741 acgctcaagt ccttggataa ggtgcgcacg gcgattcgcg cccacgagag gacgattacg
    10801 ctttccgtcg ggcagttgac gacgggggtg acggtgccgg agtggacggc ggtcttgatg
    10861 ctgagcaatg tgaagagtcc gtcgctgtat atgcaggcgg cgttccgggc gcagaatccc
    10921 tgcctgttcg tggaggggga cgagttgcgg cgcaaggaga atgcgtatgt gtttgatttt
    10981 gaaccggcgc ggacgctggt gatttttgag cagttcgcca atgacctgtg ttcggcgacg
    11041 gtgagtggcg gaggcgatgg ggagacgcgc aaaaggcagg tgcgcgagtt gctgaatttt
    11101 ttccccgtct atggggagga tgaggagggg gagatggtgg ctctggatgc ggagaaggtg
    11161 ttgtccgtgc cgcgcaagat tcatgccgtc gaggtggtgc gccgtgggtt catgagcaat
    11221 ttcctgttcc agaatatcgg gaatattttc tcggcgccgc gtcaggtggc ggatatcctc
    11281 gggcaactgc cgacggtgaa ggagcagaag ccgtccaagg cgaaggaggg cggtcaggga
    11341 gagggcggtt tgttggacgg cgccgcggat ttgcccctga atgaaaaggg tgaagtggtg
    11401 ttgcccttgg atgaattgac ccgtaagaca ggggagcttt tcggggataa gatatacagc
    11461 gacaatgttg tgacgcaaac gaagatcgat gaggtctcga cgagtaacgc gccggataag
    11521 aaggccgcac aaaatttagt gacgatattg gtggatactg tggtgaaccc caccctgcag
    11581 gatgccaaga cgcactatgg cgatgagatg agtagagcgt ctgaacatcg tctcaaaaag
    11641 cagttgcaga atcaggcgac ggtcgcggtc ggcaaggcag cgacgacgca caagaagaat
    11701 cgccacgaga cggagaaggc aaagaaaagg gctctcgacg aggcgcatgc caaggggcag
    11761 agtgaggccg aggttcaggc gattcaggat cgttttgatg ccgagcagag ggagaatgag
    11821 cgtcggtatc gagaggagtt gaaggaggca gcggcagaga ttgagaagaa ggccaaacag
    11881 gctgtggtgg atacggtgga aaccgagaag gttaccagga agaaggaggc gattgaagag
    11941 gatattcgcg gtcatttgcg tggctttgcg cggacgatcc ccgccttttt gatggcgtat
    12001 ggcgacgaga atacctgtct ggataatttc gatacgattc ttcccgatga ggtctttaga
    12061 gaggtgacgg ggatttcttt agagcagttc cgcatcttgc gcgatgggtg cgattacgaa
    12121 gatgagggtg tccggaagca ttttgacggt cattgttttg atccgattgt ttttgatgat
    12181 gccgttcagg agttcttggc gttgaagaag aaattaggca attattttga tgagtcgcag
    12241 tcggaggata ttttcgatta tattccgccg caaaagacga atcagatttt cacgcccaag
    12301 catcttgtca tgaagatggt cgatatgttg gaggaggagt ctccgggctg tttcgatgac
    12361 cccgacaaga cgtttatcga cctctacatg aagtcgggtc tatatgtggc ggagattgtc
    12421 aaacgccttt tccgcagcga ggcgatgaag gcgcgtttcc cgcaggcgga ggagcgttta
    12481 cggcatattt tcgcgcatca ggtgtatggc ttggcgccga cggagattat ttaccgcatt
    12541 gcctgtgcct ttgtcctggg cttcgacgag gagctgcgca tcgagcggca tcatctcgcg
    12601 cgcttcgatg ccttgccggc ggtcaaagac ggcacgctgg aggaggctct ggacaagatt
    12661 tgggagaggt gaccgtgatg acggcctggc ttgggaggct tcatgtcttg cgccgctccc
    12721 gttgaatgag tggcgcggct taatttccgg catgtttctt tgtgtttcat gtgcgcaagc
    12781 cgatgaaaat tcatggttgg cgtttcagcc ttttttcacg gtcaggggta gtcccgccac
    12841 catgaagtag acttcatccg cgcttttggc aaggagttgg ttggcaatgc ccagcccgtc
    12901 gcagtagtgg cgcgtttccg catctgactg gctgatgccc atgccggttt cagaagaaac
    12961 aagcacccat ggcacagggg attggcggat gagctttttc aaagcttcta tttcctcaat
    13021 cagcgcttcc tggaacggct cgtagtccgt ggggtccttc tgggcgtaca gggtattgga
    13081 ggcgagcagg gtgacgcatt ccagaatgac gccgtccacc tggtccagca agccggattc
    13141 cccgacggca gaagctacat gacggggaca ttccagcgtg agccagcttt taggacggcg
    13201 ttcctggtgc ttgcgcacgc ggtattccat ggagccggct ccgggccaga cttccgccgt
    13261 ggcgacgtac aggatttttt tcccgaatcc ggcggctatt tgttcagcgt attggctttt
    13321 gccgcttctg atgccgccca ggacgagcgt catgcgtgcg gtattggcgt cagtgtttgg
    13381 tgatgtagcg ttcatgggat tccagcatgg cctggtggga gttcaggaag tcatccaggc
    13441 tgaagacttc cagcgtaata aggggggaga aatgaaggtc ccacatgggg tgcagaatgg
    13501 cgtccagcgt agcggcaggc atgtggtgca gggatttgtg gtcccgtttt tccagcccgt
    13561 gcaggtggca catgcgtatg tccggcagcc agagcggcag aagtttttcc ggccgcaggc
    13621 cttctttcca gacatggcct atgtcaaagc aacgtgaatg gggtgtagcg gctaccagtt
    13681 tatccagata gtcggtcgga tggcgttcca aattttccag ggccaggcat tccggtgcgg
    13741 ggaggtgggg cgtgatttgt tccagactcc ggaggattcg ttccgtttcg cgttccgtaa
    13801 gatgggggcg catatcagga ccggggatat ggtccgtgac cacatgcatg acccaggtgt
    13861 ggggctccag ttcccgtgtc aggtcaatgg cgcgaatgat gttttccgta taacggcggc
    13921 ttgattcttc cgtggcgaag ctgccgtccg taggcaggtg gacgttccag gtaaccccgg
    13981 catctgcagc gattccggcc agttcccgta tttccgcagg ggtaatgagt tcctttccgc
    14041 attctccggc ttccagcagg agcagagcga catcatccgc gtaatggacg cattccctga
    14101 tggcgggcac gtagtagtcc gggatgatga aggaggtgca tccgatgttg aggcctggaa
    14161 cgcggcattt gtgtggagtt ttcataaagt gcagaaggtg agcaggacaa gccattccgt
    14221 acattccacg gtacagccga atacatcccc ggtaacgccg cccaggcggc ggcgcgcgta
    14281 tagaagcagg cccatggagc cggccagggc ggagagcagg gcgtacagcc cgttccatcc
    14341 tgccagagcg caggcagcca gggtgacggc tgcggcagcc agggcgtgcc cggggcgcgt
    14401 tccctgcttg aacgcttccc ccatgcctcc atgccgtgcg cccggaaagc gcatggcgat
    14461 gaatatctgg ctgcgggcaa gcaatcctgc cagagcgcac gccgtgagca gaagttccca
    14521 ggagccgtgg gccgccagag tcgccagggc ggctcccttg aacgccaggt tgaaaaacag
    14581 ggccgtgccg ccgaaggtcc ccaggcggga gtctttcatg atttccagcc tgcgttcccg
    14641 ggatacttca atgggaagcc cgtctccgca gtccgctact ccgtccaggt gcaggccgcc
    14701 cgtgacggct acccagcagg cacagccgat aacgccgcag accagggggg ggaagagcag
    14761 ggttgtcgtc caggtcaggc cgcccgcaag cccccccact accaggccaa ccagcggtag
    14821 ccaggcggaa attccgttca ggtcattttg cagcggccac ggccctacgg gcagccgggt
    14881 caggaagccg aaggcggagc ggatggtggt gatgacagcc atggatacag ggaagaaagg
    14941 gtcagcgcgg aacggtgatg tccagttccg gcagggtttt catttcagaa agtacgcgcg
    15001 tggcggcgtc cagcagggga aagaacaggg ccgctcccgt gccttccccc aggcagaggc
    15061 ccaggtccat gtacatggtg acgccgatgt ggtccataat gagcttatgc cccggttccg
    15121 cggagttgtg ggagccgatg aagtattgcg ccgcttccgg gcgaatgccc tgggcgatgg
    15181 ccgcggccgc cccggcaatg aagccgtcaa tgacgatggg aacgcgcagg gaggccgccc
    15241 ccagcatcag tccggccatg gctcctattt ccggtcctcc caccttcgcc agcacgtcaa
    15301 tggcatcgga agggtctggc ttgttcaggg cgattccctg ttcgatgagt tttatttttt
    15361 tgcggatgac ttctcctctg acgccggaac cgcgtcccgt cacggtttcc acaggggtgc
    15421 ccgtgagcac ggcgacgatg gcggaagagg gggtggtgtt gccaatgccc atttctccgg
    15481 cggccacaat gtccagccct ttgttttttt cttcacgggc catgtcaatg cccacttgca
    15541 ggcatttcag ggcctgttcc cgcgtcatgg ccggccccct gctgaagtcg ttggcgccgt
    15601 gcatgacttt tcttttgacc agccccggca ggtcgccgaa gtcgtaattg acgcctacgt
    15661 ccactacgga aaggcgtgct cccgcgttgc gtgtaaaggc gttgatcgtg cctccgccct
    15721 gaaggaagtt gcgggtctgg atgtaggtaa cgtccgtgga tgtcaggctg aggccgtggt
    15781 cggcgatgtg gtggtccgcg gcgaacagga ctatggcttt gtccttcagg cgcggcgcgg
    15841 gattgccggt catgccggca atacggatgg ccacgggttc cagttttccc agggcaaggg
    15901 gaggcttggc cagaattttc tgatgttcca gggccgcttt tgcggcctgt tcgtccaacg
    15961 gggggatatg cagtttgttc atgatgtcca gaagtggggg gtgaagagaa gaaggagagt
    16021 gaacgccagc gtacccgtgg cggcagtcgt ccacatgagc cgaatggcca gggccaggtc
    16081 gctggcggag gctttggttc gccctgttcc tatccagggg tagtttgcag gaattccctt
    16141 gtaagagacc gggcccccga gacggaggcc cagcgctcca gcgaaggccg cttcgctcca
    16201 tgcggaattg gggctggcgt gggcgttccg gaaggcccat cccatggtga gcgccctccg
    16261 ggcggaggtt ccttccacca tcagggcgga aaaggaaata cacggtaaaa taaggcgtgc
    16321 ggggatgaag ttgagcgcgt catccgtgcg tgcggcaaag gtgccgaagt gtctgtaccg
    16381 gtcatttttt ttcccccaca tggcatccag gatgttgaaa acacgatgcg ccagcgcggc
    16441 ggaagccgcc ccggaggcac ctcctgccag gcagcctgct gaggcccaga acagggtgga
    16501 aaaaacgcca tccgtcaagt tctccgccac gctttcaatg gccgctctgg cgatgccgta
    16561 gacgtccatc cgttccgtat cccgtcccac gatcatggag acggagtgcc gggcctctcc
    16621 ggcgttcccg ctgcgcaggg cgttttccac cttccgggca tgctcggcca ggctgcgcgg
    16681 ggccatgcag atgtaaatga ccagaacgga cggaatccag ggtgcccagg gggagggtaa
    16741 cagggaaaag ggaaggaaga gcgccgcgca gccgaaccat gcggcaatgc aggttgccag
    16801 cgcggccgcc attcccgcat gaaaagttcc tccccacctt tgcctcgcca gtgtttccat
    16861 gcggagcgcg caccggccga tcagacagac gggatgaaac ctgttgggcg gttctcccgc
    16921 cagaatatcc agcaggaagg ccgcaggcag gatgaacggg cacgggaaca tgggtcagcg
    16981 ttttagttgg agagctcggt aaatggtttt caggtccaga tgtttccgga ccacgtccgc
    17041 caggcggtcc agcgctccgt ccaggtcata ggcggtgtgc agggctgggt tggctttcag
    17101 ccctgaatct ttcctgacca tgttgatgaa tgcgcggcgg aattggtctc cgtccagcat
    17161 gccgtgcaga tacgtggccc agatacggcc tttgccgtag ccgcaggggg agccgtcttc
    17221 ccggaacatg acgggggggc tggattcttg atggctggtg acgccatggt ggatttcata
    17281 acctgcggcg ggaacaggca gcggcgtgct tgcccggcgc acgttgatca gggttttggc
    17341 tgaagagaag gtggtggaaa gttccagcag ccccagtcct ggcgtgcgct cctccgggga
    17401 ttccatgtgc agcgggtcca gaatgtccgt ccccagcatt tgcagccctc cgcagacgcc
    17461 cagaagccac tttccctttt cggcatgacg gcggatgggt tcttccagcc cggcggaacg
    17521 gagagaagcc aggtccgcag ccacgctttt agtgccgggc agaatgacca ggtccggatt
    17581 tccccattcc tcccgtgtcc gcacctggcg gagccggacg tccggctccg ccgccagagg
    17641 ggcgaagtcc gtgaagttgg agacgtgggc gggcatgact acggccacat ccaggcaatc
    17701 cgcatgtttc ccgtgctccc cgtggccagg gggcagcgtg gcgcggtctt cttccggaat
    17761 gttgatgtcc cgcatcatcg ggatgacgcc cagcacaggc ttgcccgtac gattccgcat
    17821 gtagctgtgc gccggggcca gcagatccgg atctcctcga aatttgttga ccacgaaccc
    17881 cgccagcagt tctttttccc acggggcgaa tgtcatccac gttcccagaa aggaggcgta
    17941 cactccgcca cggtcaatgt ccccggcgag caggactctg gcacgcgcgt agcgggccat
    18001 gttcatgttg accacgtctg ctgatttcag attgatttct ccggggcttc cggccccttc
    18061 caggcagagg agttcatatt cgtccgccag ggaatcgtat gctttgcata cgtccggcca
    18121 gaagcgcctt ttagccgtga agtattcccg cgcgtccatg cggcctacgg ggcgccccat
    18181 cacgatcacc tgggagccgg tattggaatg gggcttgaga agaatggggt tcattctggc
    18241 atccggatca atgcggcagg cctgggcctg caccagctgg gcgcggccca tttcctctcc
    18301 cagcgcagtt acgccggagt tgagagccat gttctgcgcc ttgaacggtg ccacgtgata
    18361 gccgtcctga aggaaaatgc ggcacagggc tgccgtgaga acgctttttc ctgcatcgga
    18421 gcaggtgccc tgaatcatca gggccggttt gggagcctta cggataatgg aggggccgtt
    18481 tcccgccagt tcggcgcgca gagcttccgc cagcagggcg tgttcctccg gcgtgcggac
    18541 gccggagcgc aaccagcagc ccgtttccag acccggataa ttggaacagt cgcgcagtgc
    18601 gatgccgtat tttttcagga gccgggctgc caggccatga ggcgcccccg ccaggcggaa
    18661 gagcaggaag ttggcctcgg acgggagtac ggcggcgccc ggcagggagg agagcctgcg
    18721 gaacaggtcg tcccggcgtt cccggttccg ggcgcgttcc ctgtctgccc aggaggaggg
    18781 ttgtgccagc acggccttca ccgctgccgt cgcaaaggca ttcacgttcc aggcgggcag
    18841 ggatcgccgc agccgctccg cgagcggagc ggcacagatg gaaaaaccgc accggacgcc
    18901 ggccatgccg taaaatttgg tcagggagcg cagaaccacc aggttgggaa ggagggccgc
    18961 ttcacggagc agcgattccg ctccttgtgc gtaatccatg aaagattcgt caatgatcca
    19021 gaggacttcg gggcggctct ggacaaccct gtgcagggcc gctgcgtcca gcaggccgcc
    19081 ggacggattg ctgggatttg ccagaaaaac agcgcttccg tctgcggcct gttcctccag
    19141 acggcaaaga gacgggagga aggaattccg ttcctccgtc cggatggaaa gaacatccgt
    19201 gccgtggcgc aggcaggcca gcctgtattc ggaaaaagcg ggttccggaa tgacggcctg
    19261 tttaaggttt aatgcgcgcg gaagagcgtg aatgagttca ttggccccgt ttccaaatac
    19321 gaagcagccg gaaggaagcc cgtaatgaac cgccgccagt tcccgcagat ccgccgcatc
    19381 cggagaaggg tagggcactg cgttttccat ggccttccac aatgccgaag cgatgaactc
    19441 cggcatacct tccggcctca ggttgacgct gaagtccaaa atatcccgtt ccgggcggca
    19501 ggcctcctcc gccagggatt ttaaatctcc tccatgtgaa aaaacgttca tgccacagta
    19561 attggaaccg gagtaccgaa gggctgcccg cgccggagaa agaaaggaag cgccttgcaa
    19621 gttcggaatt ccacatagcc ggaagcttcg ccggagacgg gaaatcttcc ctgttgcggg
    19681 aacgtgggaa caaggagcgt catgagcgca tcatgtcctg tatgcgccgg gagtgacaag
    19741 acaaaaatgg aaagccccgt gaggaaaaga gggatggaga gcaatacgct gaaaagggaa
    19801 aacgactgca attcatctgg atgttcaaag aaaaattttt ttgtcttgtc ataccggtct
    19861 ctttggtatt tcatccgtcc catgaaccct tatgggaagg gttacagacc tcagggatgc
    19921 ccgtagccgg gcaggaaata aatggggaat accggtgtga gtccggagcg gtaccgccac
    19981 tgtaagcgag cctgcctcgc aagccagaaa accagcctgt gaggttgtcc aactgttcgg
    20041 cgagttaccg aacgtaaggc actacaacca tgctttatac gaagaaagcc cttcaaatgg
    20101 gcgcaattgc tgttggcctt gccgcattcg caggccagtc ctctcttgcc gagtccgcta
    20161 aaaaggaaga ctccaagccc tccgtcagga cggaaaccat gcaggttatg ccggaactga
    20221 ccatggcgtc ccactttgtg ggcgtgccgt acaaccgttc tggagtgtcc gtttccatca
    20281 tcaatccgga agaattccag aaggcgggca ttgaaaccct gacgggagcc ctttcccaga
    20341 cgcccggcgt tttcacgctg gacggaggcg gcacctggca gcgcggttcc gtgagcaaca
    20401 ccgtcatccg cgggatgaac aaggatacct acactctgac catggttgac ggcatgcgca
    20461 tcagtgatgc caacatgtcc ggcaacaagc tgctgggcat caccaatctc tttacggtgg
    20521 gcaatgtgga agtggtgaaa ggagcgcagg gcgccgtttt cggttccggc gccattggag
    20581 gcgttgtcgc gatggatact ccggaggggg aaggcgatcc cgtaaccagg atttttgcgg
    20641 aggccggttc tttcggctct ttcaacagtt atgtcacttc ctccggcaag atcaagaagc
    20701 tttcctattt tgtaggcgtt gggtttgaaa ctacggaaaa cgatccgtca atctatccgg
    20761 ccatttatga caacaggaca ggcatgaacg atttccgcca gtggcaggaa gccgtgcgcc
    20821 tgggctatga tatcaatgac aaggtgaagg tgagctttac ctatcgccgc ctggattcct
    20881 actttgaata tcccacgccg tatgtggatt ataaccagtg gccttccgtt ccggatcccc
    20941 atctgtacaa tacggaagac aagaaccgca gcaacctggt gacaggacgt gtggatgcgg
    21001 aaatttccaa gctgtggtcc accagcttca tggtggggca ttacaacatg gactattcct
    21061 gccatactcc cggatttgac ttccagccca acgtgatgcg caaccgccgc ttccaggcgg
    21121 agtggcgcaa tgccctgacg tggaacaagg aatggaaaac catcgccggc atggcctggg
    21181 accgttccga ctacatgagc gaaaacaatt acgttgccaa ggatgaatgg cagagcacgc
    21241 ttgccttctt tgcggagcaa atgtggtcac ccacggacag ctttgacgcc agcgtggccc
    21301 tgcgcctgga acatgattcc gtctggaaca atcattttac gtggcgttat tccaattcct
    21361 ggaaagtgac gggcaaggat tcccccaccc gtattttcgg ttccgtaggg tcaggtttcc
    21421 gcgcacccac ctggtttgag cagtatgcgg ccaattacgg ttatgtaggc aaccctgatc
    21481 tggatgtgtc caagtccctg ggcggcgact tgggtgtgga acagcgcctg gcggacggcc
    21541 attatgcttc cgtgacgggc ttctggaccc gcattaacga tgaaatcggc accaagagcg
    21601 taggcacctg gccgaactcc tataccactt acgccaatta ttcccactgc acttcctacg
    21661 gtgtggaagt ggcgttcaag ggtcagttca aggacgcgtg gaacagcggc tattatgcca
    21721 attacacctt cacgatgccc aagcgcgatt ccatcggcaa atacgagacc attcaaatgg
    21781 ccaataccgc ccgccacacc gtcaacgcag aggtttatac ctccccggtt gaaaagctga
    21841 cggtcggctt cggcgtaacg gccgccatgg gacgcacgga ctacaactat gcccgtctgg
    21901 acaatttctt tacggcgcgc ctgtttgccc gttaccaggc aacggacaat gtgtccctcc
    21961 atgtgcgtct ggaaaacctg tttgaccaga agttcatcat gacgaatgat tataacttcg
    22021 gtccgcgtga agcccgtgga ttcggaatct tcggcggtgt gacggtcgaa ttctaagttc
    22081 ctttcaatat taattgttga ttgctccccc atcttccgcc ttgcggactg tttcaggcga
    22141 agtgaacggt tccgcgcgga agatgggttt tctgttttcc gttgtttccg gaatcggtta
    22201 acatgtggcg gccatctgag ttgtgttgcg ctggatatcc atactggggg cattggccct
    22261 ttccgtcttt ctgggaggtg tcttttactt ttcccagcgt gagaagaatg tgtggcatga
    22321 attttccgga cggatggacg gcagggggat gcctgccgta tttgacggtt ttgtggaacg
    22381 ctggaacagg gccgtggaac gggaactgcg ccgtgaattg gctgaagggg agaggaagga
    22441 aaaacttctg gtcgttttcc gcggggaagg ggacggccgg cgtttggcgg aacaggctcg
    22501 ttccgtggaa cgcatcagga aaaggctgtt attgaaagac catatccgcc ttcttccttt
    22561 ggaagacgtt ccggacggat tggaatggca aaccggaatg gaagagccgg atgtgggtga
    22621 tccgcgcgcc gtcaggggtg gaaaagtccg gctgtggatc aatactccat ttcccggaac
    22681 tttacgtgct ttcgggccgg gaagtgaaaa ttttttcaat tactccgcca ttgacaacgt
    22741 gtggcttccc ctcgtggggc ttcatccgga aaccttccgt cccattccgg gcctggcgga
    22801 ccgctgggct ttgtccgcag acggaaaaac cgttttttat catctggatc cggaagcggc
    22861 ctattcggac ggacgcatcg tgaaagcgca ggattttctg ctgaacattt gcctccgtac
    22921 gtccggtttt gcccgggatc cgttctggac taccctgttc cgttccatgt atgatcagat
    22981 tacggtatac ggggattccg tcattgccct tacgctgccg tcccgcgggc cgctgctgcc
    23041 ttacatggcc tgtgcggatt ttcacccggc ccatcccggt ttttaccatg atttcaacgc
    23101 gctgtttctg gaacgctacc agtggaaagt ccctccaaat acgggtggct acatggttgt
    23161 tcccgctaaa atccggatag gagaaagcat taccctggcc cgcgtgaaaa attggtgggc
    23221 gaaggacagg aagtattacc gccattcctg caatgcggac caggtggaac atgtgtttgt
    23281 gaacatggaa aacaaggcgg ttgaaatgtt ccgacgcggg gagctggaca tcatgaacgt
    23341 tcgcaagccg gaggtgtggg aagcagggct ggagctgccg gaagtgcacc ggggctatat
    23401 agataaatac agcctggaag ccaattacgc ctgtcccccg tacggactgt atctgaattg
    23461 ctcggacaag ttgttgaaaa atccggatat ccgccgcgga ctggcgcatt ccgtgaacat
    23521 gggcctggtg attgagaccc tgttccgcgg gaatatgaga aggctcggtt cctacatgga
    23581 ggggtacgga gacctgactc ttccgctgaa agctccggaa tacagcaaga aaaaagccat
    23641 ggaatatttt gcccgcgccg gctaccggga aatggggact gacggagtgc tgaaaaatga
    23701 acggggagaa cggctggtgg tggaactgac gtttgcggat tcatccaccc tgatgaccaa
    23761 cgtctgttcc gttctccggc aggaggccct gaagtgcggt gtggacctgc gtctggattc
    23821 ccttacctac agtgtctgtt cccgtaaggt ttttgaaaaa cggtatcagg cggctctgtg
    23881 ggcgtggccg ctgcagacgc cgttcccccg gctgtatgaa acattctctt ccgaactggc
    23941 gtatgatgcc cgcggaaatc ccgtaggcaa tacgaataat attatggctg tgtcggacgc
    24001 tggactggat gccgccctgg atgcggaacg caatgcaccg gatatcggtt ccctgaagct
    24061 ggcccttcac cgtgcccagc agcgtcttca tgaactttgc gtctggattc ccggctggag
    24121 ggaaccctac acgcatatcg cctgctggcg ctggatacgc tggccggaat ctcccacccg
    24181 gttctgttcc cccagaattt acaatccgct ggagtcgcac ctgtactggg tggatgaaga
    24241 aatgaagaag gagacgctgg aggctcggag cctgggcgta ccgtttgagg aaaagaatca
    24301 aataatatat cttgaaagac aggatggtgc cgctccgtaa taaaaccggg tcaccgtctc
    24361 ctttttaaat tgaaaggagg tggtttttca tcataatctc cctctcattt accgtaatta
    24421 attattaatt ccataaccat gaaattatcc tttttctccg ttctgcttct ggcagggcat
    24481 ctttgtgcgg ctgctcccat gcctctgccg gaatccaatg acggagccag gcatgtcttc
    24541 tctactaatc aagaaaattt tttgatggac ggaaagcccg tcaaaatcat ttccggggaa
    24601 atgcattatc ctcgcgtgcc gcgccagcac tggaaggaca ggttccagcg catgaaggcc
    24661 atgggcataa atactctctg tacctatctg ttctggaatg cgcacgaacc ggaaccgggc
    24721 aaatgggact tttccggcaa tctggatttt gtggaattca tcaaggaggc gcagaaggcc
    24781 ggcctgtggg tcatcgtgcg tcccgggccc tatgtgtgcg gggaatggga attcggagga
    24841 tttcccggct ggctgctgaa ggatgaagat ttgaaagtcc gttcccagga tccccgcttc
    24901 ctggaaccgg ccatggctta tcttaaaaaa gtctgttcca tgctggaacc tctgcagatc
    24961 accaagggag gccccatcat catggcccag gtggaaaatg aatacggctc ctatggttct
    25021 gacaaggatt acgtgaaaaa gcatctggac gttatccgga aggaacttcc gggagttgtt
    25081 cccttcacgt cggacggccc gaacgactgg atgatcaaga acggcacgct tccgggcgtt
    25141 gttcccgcca tgaatttcgg cggcggagcc aagggcgctt ttgcgaatct ggagaagcac
    25201 aagggcaaaa cgccccgcat caacggcgaa ttctgggtgg gctggtttga ccactggggc
    25261 aagcccaaga atggcggcag tacggaaggt ttcaaccgag acctgaagtg gatgctggaa
    25321 aataacgttt cccccaacct attcatggcg catgggggga cctccttcgg cttcatgaac
    25381 ggggcgaact gggaaggcgc ctacacgccg gatgtaacca attacgacta cggcgccccc
    25441 atttccgaaa acggaaccct gacggaccgc taccgcacct tccgccagac tattcaggat
    25501 tattacggtg atacgtacaa gcttcccgaa cctcccgccc agccggaaat gatggagctg
    25561 cctcccatca cgtttacgga aacagccggc atgttctccc gcctgccgca gcccgtcatc
    25621 cgcaaggagc ccgtccacat ggaagccctg gggcaaagcc tgggcttcat cctgtaccgg
    25681 acaaaggtga acggcccggt gaaaggagag ctgaagatga acaacatgca ggaccgcgcc
    25741 atcgtttacg tggacggcaa gaggcaggga gcggcggacc gccgttacaa gcaggattcc
    25801 tgtgacattg ttattccctc cggacttcac acggtggaca tttttgtgga aaacatgggc
    25861 cgcatcaact tcggcggcca gatacagggc gagcgcaagg gcatccgggg ccccattacg
    25921 ctggacggca aaaagctgga aaacttcctt atctacaact tcccatgcaa gggggtggag
    25981 cttattccct tctccggcaa gaagccggcg ggcgatcagc ccgtgttcca ccgcgggtat
    26041 ttcaacgttt ccaatcccaa ggatacctac ctggatatgc gggacggctg gaagaaaggc
    26101 gtcgtgtggg tgaatggacg caatctgggc cgcttctggt ttatcggctc ccagcaggct
    26161 ctttattgcc ccggagaata cctgaagccc gggaaaaatg aaatcgtggt gctggacgtg
    26221 gacggaggtt ccggcacggt gaagggtgtg aaggaagcca tttatgaagt caacagggac
    26281 cccgccatgg cggatgtctt ccgcgtgggc aaacctgtgg cccccgctgt cggccagctg
    26341 gtgcacaagg gttccttcgc caagggggcg gaccagcagg aaatcaaatt ccgcgctcct
    26401 gtccaggccc gttacattgc tattgtcagt aagaacgctc atgacaacgg cccccatgcc
    26461 gccattgcgg agctgaactt cctggatgcc tccggcaatc tgctcccccg cgaacagtgg
    26521 tccgtggttt atgcggattc ccatgaaacg acgggagaag ccgcccaggc gggactggtg
    26581 atggacaacc agcccaccac ctactggcat accaagtggc agggggacaa ccccagtcat
    26641 ccgcacatga tcgtgctgga tctgggcaag gtgcagaaac tttcaggatt ccgctacctg
    26701 ccgcgccagg accgggaaaa cggccgtatc aaggactatg aagtctatgc gtctcccaag
    26761 ccgttcaagc ctgccaagta agatcctctg aattgctgaa ctttccaggg nnnnnnnnnn
    26821 gtccggatat cccggaagaa aacagtcccc ccgcttttcc cggacaagag tagcgccggt
    26881 ttaaagatac gtatcaactt ttcgtttccc ttgttttctg ctgttgtgcg ggccggtatt
    26941 ccatgcgcaa aaaaaggcgg ccccacagtg taacggagcc gccggaaggc agtaaaaacc
    27001 tgatagccgg ttgttagaag ttccaggaaa agcccagctt gatggtgtgg tagccgggat
    27061 cattataacc ctgaatctgg tagtattcgt agccgatcag gaagtccgcg ctgttggaga
    27121 tgctgaactt gaaaccggcc tgggccgccc aggaaaactt ggtggaggaa atggtgcggg
    27181 aacggctgcc atcttccaag ccatgaatgg tggtcttcac gtcgccgtaa gtggcgccga
    27241 ctttaccgcc cagatagaac atggtgtagt cgccgatggg aaggttcagc gtgtaaccgg
    27301 ccatcatcgg aatataggtg gaacggaggc tggcggtata aggcccgctg atgcccaggt
    27361 cctgcacgct gggatggtgg gaaccggcca ggatgccgcc gttcagggaa atctgatgaa
    27421 caatgctgcc ggtttcaata tagttgaaaa tgctcagatt gccgccggcc acgttgggca
    27481 tggcatcttt ggtggcgatt ccgtaagccc cttccaggga catgccgaac tggccgtcgt
    27541 acttgtcggc atgataggcg ctggcggaag acacggaggc cgcaagggcc aaacctaaca
    27601 gtatagtctt gttcatatgg ggataaatgg taaatttaac tgtagtccat tcttggcagt
    27661 tttctctttt gaagtcaaac atgaaatgtc aaatggacgc gaaatccccg agccgaagag
    27721 atgaagttgc cagaaccctc ccgctggggt acattctgcg ccatggaaca acaagcgtgt
    27781 gatttgctgg tgaccgcttc ccgcatgctg ggtggagcgg aacagtccgt tgctcccggg
    27841 gatgcggccg tcgccgtccg ggacgggaaa attctggaaa cgggaagcgc cgcagaactg
    27901 gaggccaggt ggaggcccgc cgtccgcagg aatttgggga atgtgctgct gatgccgggg
    27961 cttattaatg cccatacgca tgtccccatg acctttttgc gcggttttgc ggatgacttg
    28021 ccgctgatgg aatggctgac cggacatatt ttcccagtgg aagcccggct gacggataaa
    28081 attgtctccc tgggggcccg gctgggcatg tatgaaatga tgcggacggg caccacggcg
    28141 tttgtagatt cctacctgtt ggaagccaac gttctccagg aggcggaacg catgggaatg
    28201 cgctgtgtgg gcggagaggt tgtttttgcc tttccctctc cggcgtacgg cgggtgggac
    28261 ggtgcggaag ccctgtaccg ggagcaggcg gagcgctttt ccggccgcgg cagggtacag
    28321 gtggccctga tgccgcacag cgtgtacacc accagtgacg aagtgctccg gcgttccatg
    28381 aaactggcgg aggaactgga cctgatgctg catatccatt tgtccgaatc cgccggggaa
    28441 gtggagcagt gctgttccct gcacgggggc aggcgtcccg tgggctatgc ccgtgatatg
    28501 gggctgctga atgaacgcgc cgtgctggcc catatggtgg acgtgacgga tgaggaactg
    28561 gaactggtgg ccgcttccgg agcggcggtt gtccataacc cggtcagcaa cctgaaactg
    28621 gcgtccggct tcgcccgcgt gcgggatatg gtgcgggccg gcgttcccgt ctctctggga
    28681 acggatggag cgtgcagcaa caacagtctg gacatgtttg aaacaatgaa gctggccgct
    28741 attcttgcca aggggtacag cggggacgct actgcggtcc ccgccatgca ggcgttgaag
    28801 atggcgacgg aggagggcgc ccgcattttt cggacgccgg ggctgggcac actggttccg
    28861 ggggctcccg cggatatgat cgccctgaac ctggatgaac cgaacctgtg cccgattttc
    28921 aacgaaacgt cccatgccgt ttatgcctct tccggcaagg attgcgtgtt caccatggtg
    28981 gaagggagaa ttttgtatga tcatggaatt tacacggacg gcctgtatgc ggataccgcc
    29041 gcggaaatgc gggatctggt aaagtgggtg aagaactgtg attgagtatt ggggatgagc
    29101 gaataaaggt aggttctcaa cacatgcttt aatctctggg cccttacctc tgctatctgt
    29161 cggcctcccg aaccggcgca tgctcatatt ccttctttac ccgcaagggg ggatgccttt
    29221 atagtaattt catgcaatat gatcttatcg tcattggtgg aggccctgcc ggctatgtgg
    29281 gcgccatccg cgccgcccag ctggggaaat ccgtggtgtg cgtggaacgc gaccgggtgg
    29341 ggggcacctg tctgaactgg ggatgcatcc ccaccaaggc gcttctgaaa aatgcggagg
    29401 cctaccggat cgtgacggac cgcgctaggg aattcggcat gacggtggag ggcgtcagcg
    29461 tggattggag tgaagtgatc ggccgttccc gcaaggtcag cgaccgcctg gcaggcggcg
    29521 tggggttcct cttcaagaaa aacaaggtgg attccgttac gggggaagcc tccatcattt
    29581 cccccggcag ggtggaagta aaggcagccg acggaacggt gaatgttctg gagggaaaga
    29641 atatcctgat ctgtaccggc tgcgtcaccc gcacggtgcc cagccttccc ctgaacggca
    29701 ctactgtcat cggctccagg gaagccatgg tgctggaaaa gcggccagaa agcatgatca
    29761 tcatcggttc cggagctatc ggcacggaat ttgcctacat ttataattca ttcggcacca
    29821 gagtgacgct tattgaagcg cttccccgga tgcttcccaa tgaagacgac gactcctgca
    29881 tgacgctgga acgggcattc aagaagcagg gcatcaaggt gatgacggga gcttccgtgg
    29941 aatccgtcac ggaaacctgt gacggacagg tcagggccag cgtaaaaaac agcaggggac
    30001 aggaggagga aatcacggcg gacgtctgcc tggtggccat cggcgtgaag ccggtcgttc
    30061 ccgccgctcc cggcctggaa ttgacggaaa aaggattcat caaggtgaat gaccgctatg
    30121 cgacttccat tcccggcgtt tatgcggcag gcgacgtgat cggcggcgtg ctgctggcac
    30181 atacggcttc ttttgaagct gtccaggctg tggagggcat gtttaatccg gactaccagt
    30241 cccggcaagt cggcttcttc ccgagctgca cgtactgcta cccgcaggtg gcttccgtag
    30301 gtaaaacgga acgcgccctc aaggaagcgg gcgtggaata caaggtgggg aaattcccct
    30361 tccaggccat tggcaaggct gtagccgccg gagagccgga cggcttcgtg aaaaccctgt
    30421 acggcgccaa aaacggagaa ttgctgggtg cccatatcgt ggggccagaa gccacagaac
    30481 tgattgccgc gctgggcatc ggcattcagg cggaactgac ggatgaagat atccacgcca
    30541 ccatttttgc tcatcccacg ctgtctgagg ccattcatga atccatgctg gcttccgagg
    30601 ggattgccat tcacatgtaa cggttttcaa tggaagagcc ccccctccgg gggctcccat
    30661 tgattccttt gtcctgtaaa aggatgaata tcaatctgcg ttagaaaaag acgctaaagg
    30721 gtgaatttgt tgatgacggc tacagtccag cagttcccgt gaaggctcag ataggctttg
    30781 agctggctca atagtcaatg tagatggtcg attgcaagcc ttggaaggcc gcaatttgcg
    30841 tgtacatctt tacgatgatt cctttgggga gaaaaagcgg ggttccctga ttaaggagaa
    30901 atttcctccg tgctggatgg tgtgcgggaa atatgaatgg atgagtagat gataattttc
    30961 gtagccataa ggggataact gatttaagca acaaatcctc atgtgtgaat atgcatggaa
    31021 gggcaatttt caaagaaaat attgcttgaa tttccgaatc agataactta tgagaaaaac
    31081 attccgcttc gttttcagga gattcaaaat gactaaaaat atactactcg cacctattct
    31141 gaaatgggtt ggaggcaagc gtcaactcct aaatgacatt cttcccctaa ttcaatccaa
    31201 gggaacgtac atagagcctt ttcttggtgg gggtgctgtt ttattcgctc atcaacctaa
    31261 gaaagctatc gtaaatgact ataactcgga attaatgaat gtttatcaag ttgtaaaatc
    31321 ttatccagag caacttatag aattattaca aattcattat gctaacaatt ctgagaaata
    31381 tttttataaa ataagagaac ttgacaggct ggaaaaatat caagaacttt ctcaaattga
    31441 aaaagcatca agaatactct atctaaataa aacttgttac aatggtttat atagagtaaa
    31501 ttcttctggt tatttcaatt ctccatatgg aaaatataaa aatccaaata tagtaaatgc
    31561 tcccgtaatc agggcaatgt caaagtattt caatgacaac gatattactt tactacaggg
    31621 tgattataag gaagtattga agaaagctag gaaaggaagt ttcgtctact tagacccacc
    31681 atatatgccg atttccagtt cttccaactt cacaggatat acagaaaacg gatttgggta
    31741 taagcagcag gaagagttga agaaggaatg tgacaagctg agagagaaag gtattcggtt
    31801 tatacaatct aattctgact gtccagaaat tcgtgaactt tacagagagt atacggttaa
    31861 agtcgtgcaa gccaaacgtg ttatcaatag caattcagca aaacgtggtg aaattgacga
    31921 agttttgata tatggctgat gaaacttcca taaaaatgac agcggaagaa gcatggaata
    31981 ttttattgaa caagtataat attgttcaag aagtnnnnnn nnnnnnnnnn nnnnnncagc
    32041 caaattaatg actataaaga agctagattg atggcgaaat gggatacttc agagagtctt
    32101 cctccttctt tgaaatctaa taaactgaat attcttcctg atagcagaag ttcttacgtt
    32161 atcggtgatt tcatattata tgaggaaatt cctgaattaa cagaacgagt tgaacagatg
    32221 acgcaagttc aacttggaga atatgaaacc atagatgtta ataacataac ttccgaagcg
    32281 aacgcaatta atgttcttgt tttgtctgga atattagacg attttcttga aacatccgat
    32341 accgcagcga cctttaatgg gcgtatgggt actggatgct ttaattttga ggtaaatact
    32401 cacaggaacg tgaagcgtaa aattaacgta aataacgctc aatgtgagat agatggtggt
    32461 tttgagaata gtacggaagt tgttattctt gaagcaaaga atgttttgca caaagatttt
    32521 cacgttagac agttatacta t
//
LOCUS       DAWEMX010000011        29384 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_647_length_29384_cov_12.146510, whole genome shotgun sequence.
ACCESSION   DAWEMX010000011 DAWEMX010000000
VERSION     DAWEMX010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 29384)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29384)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29384
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_29384_cov_12.146510"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    682..691
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15176..15199
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5881 a         7749 c         9182 g         6538 t
ORIGIN      
        1 gcgcggctga cgcccgcacc cgcgcgcgcc tgctgctgga aatcctgatg gcggaacggg
       61 cccggggcgg caacaaggcc tttctgcagc gggcggacgg catccggggc ctcatgtctt
      121 cctccgcttt tcttccggcg ctggaggaat ggcttgccag ggtggctccg ccgccggccc
      181 cgccggaaaa ataaattaag gacgtatggc aaatttggat tgccttttag gggattatgg
      241 ctcatattct gcgtcccgta agttttcctt ttactgatat tcaccagcaa tagataacac
      301 aagatgaaca ttatcaacaa aatcgagcaa gagcaactca aggcggatgt gaccccattt
      361 aacgtaggtg ataccatcaa ggttcacact cgtgttattg aaggcggcaa ggaacgtatc
      421 cagattttcc agggcattgt gatcgccaag cgcggttccg gcatcaatga agctttcacc
      481 gtccgcaaaa tttcctacgg tgaaggcgtg gaacgcgtat tccccctgca tactccccgc
      541 attgccaaga ttgaagtcgt gaaccgcggc aaagtccgcc gcgccaaact tcactatctg
      601 cgcggccgca tcggcaagga tgccatgatt gtgaagtctg ccaaccggta atcggaagaa
      661 ttgctatttt gctggcccgg cnnnnnnnnn ntccggagta ttgtttttcc tgagtgggaa
      721 agaggtccac agggacgaca gccgcttcag gatatttccc tgggagccat atggggatga
      781 gagcgttttt ccgggttggg gaaggtggag aagggcggct gtttccggtc cggagcaaat
      841 acgtttttcg gattttcctc cgtccgggcc tggatggctt ccggagaatg gagcagaaac
      901 cggaaaattt catacgcgaa gccaatcccg gaacttagcg cgtgacattc catcaggccc
      961 tgccccaacc tgctttccgg gaaggggaaa ggaaacggcg gttttcagca tagcctccgg
     1021 agcgggtgca gggctgcgcg gataattatt tttccgcagg tgcgggagcc ggtgtgtcct
     1081 ccgcatcttc cggtccgtac gcttccactt ccgtcaacgc cagcggctgg gagggatttg
     1141 cggattccac gcgcagggct cgcgccttga ccggagcgtc cagtttccag gaagtgacgt
     1201 tggtggattt ttccgaagga gtggtgctga aggttttttc cgccagcacg tttttgtcat
     1261 tgccgtagag gattaccttg aatttttcca tggtgcccag cagcgtcagg ttggccggcg
     1321 tgtaaatgac caccttttct atggtgcggt tggtctccct gccgaagtca atgccgaacc
     1381 aggcggtttt cccttcaacc acgcctgtgg agctgattga aacgtcggaa atcctggagc
     1441 tggtattgcc gtcaacggcc ttttccggac cgtaagctcc cagcatgttg cggttggaac
     1501 tctggcgaac cttgacgccg ggttgaaggc tccaattgac tttgccgggt tccgcggaaa
     1561 ctttgggaac tgccggcgcc tgcgccggac gcgcggccgg aacaggtgaa ggaatggccg
     1621 cagcggcgtt ttgcgcagtg gccggagcgt ggcggcgcgc gtccctgttc gcttgtcttg
     1681 ccgcttccgc tttcgcgtta ttttcttttt gcacctgttc attataatca ttgcgcattc
     1741 taacgacatc cttcttatac aggatgcagg tataggtcag cacaatgccc acgatcaggg
     1801 ccggaatgag atagtaatag atgttggact gccctttttg catgttcagg gccggaatgg
     1861 ccggggaaga tggcggcgga gggatggagg ccgcatttct tcccgcagga ttggcaatgg
     1921 ctttatccag atccgtgatg aaatccccca ggtcccggta gcgttcccgg gtatccggat
     1981 tgatggcgcg ggagactgcc gaatccacgg catcgctaca tttgattagt gaggagggca
     2041 tggcaaagcc cgcttccatc ggcgttttgc gggtaagcag aatgtaaagc gccagcccca
     2101 gtgcgtaaat atccatgccc ggcgtgaacg tggtttccgc tccggtgacc cactccggag
     2161 aggcgaagtc ccccatgtcc accggaggtt gggcgggata aatgttgatg ggcagcagca
     2221 gggcgtctcc gccgtcttcg cgcaggcaaa tcagcttggg cgtgatgccc ccgtgaaaaa
     2281 tgtttttccg gtgcagtgtc agcagtcctt ccgccatgtt gcgcaccagg ttgagcgccg
     2341 cctggggaga tatttgggga agggtttcca tatccgccag acgcggatag ggggagtgtt
     2401 cggagatgat gaaagtaaac ggccccgcct gtcctacttc ataaacgcgc aggagcccct
     2461 ggtgggattg ttccacgatg gtgagggagc ggacgatgaa ttcaggatcc cagccgaaga
     2521 tgccggcttc ttccgtggga accacccgca gcataacggg agcaaaattt gatttcaggg
     2581 tagctgtgaa aatttgctgg gggcctttgg ctccgacaag ctggatattt tccaattggg
     2641 gaaagagaga ggaaatctgg tccatgggaa aggaatcgtc ggcagacatg gaaaagggag
     2701 gtggaatagt attgattccc actctacctt aaattcccct acggaagcaa ctctataagc
     2761 gggcatattt tgatgtcggg acgccctgat gctgaacgga cgccaatcat gcccggatgg
     2821 agcgcgccct tttccgtaac cggccagtca aatcagggca gccaggaaca ggccgtgatg
     2881 agcagggaaa atttcagttg tttcaactgc ggcggcatgt tgccgcagat gcccgcggca
     2941 aggtaatcct gtccggttcc tgtttttccc ggaagcggca gggagggcgg atgaacgagg
     3001 gcgctccagc cgttaaagcc gggacataca ggaacccgga aactggaccg ccgaggcgtg
     3061 gccgtttgac tggaagatgg ctgccgctat cagcaggaac aacagaaggg ggaagggaag
     3121 gaggccttgc atggacaagg agaatcagcc ccctgcatca gggccttttc aggatgatag
     3181 gcggtgaggg attggcctgc catacgggag tggcggcttt cgcgtccgct tcttcgagcg
     3241 ttttacggcc tttgaccttg accaggtgtt cccggaggat gcgccgcagt tccacggcgg
     3301 aggccgggtc atcctgcacg ctgtgcccgg atggaacgat tttttcactg gcgctccagt
     3361 ccaggtggga tgagtggtac ttgaccactc cgtcggagga attgggagtg tcgttcctgc
     3421 ccctgtctcc aatgatggaa tgaacgggaa catttgggga gccgcgcagc tccgacatgt
     3481 atttgaccag ggatgaattc ggggaaagct ggcggatgct ggtcagctcg tccttcagtt
     3541 gcagggggtt aaggaggaac atgccgcggc tgagagtggc gatgcgggtg acttcttcca
     3601 gcatgtccga ggggaggtcg atcaggtgct gtcccagcct tcctatccag ttgtccgcga
     3661 aagtggaacc gcggtgcggg gtggccatgt acacgatgcg cgtgacgctg ctggacgggg
     3721 agaagatgaa attgttctgg agctgttcaa gcgtgtgttc gtcacagttc aggggcgcca
     3781 gtccgttttt ccagtttccc atcgtcatgc ctgcgaaggt ttccggggga agttcaaaga
     3841 ttcccttcat cagcgtccag ggtttggtgg agttattgaa gcgggtgatg agtccgccca
     3901 tggagtgccc caccatgacg atgttgttca tttccctgga tgtcccgcgg ggattgtatt
     3961 cctgcatgag ctccttcagg gcctgccgct gcctgctggc cggaatggtc caggccagcc
     4021 cggacggata gccgaagaac cagaattggt agttttcccg gatgaccgga tccaccagga
     4081 gacggttggt caggttagcg aaggtggcgg ggccggacat caggccgtgg gtgaaaacca
     4141 cgggtatttt ccgggggtca tagggttcgg aaaaataaag ccccatcgta ttgatggcct
     4201 ttttggggcg gaatgccccc agcagttccg tcccgtctgc gtcggaaagc ttccagaaga
     4261 gagcgatggg gacggaaaaa ttggccgcca gaggctgccg caccttgttt ttgccaatga
     4321 agatgtgctc attcatcagg cgcggaatgg tgcggagggt aggtttcccg tccaccatgc
     4381 ggtcaaagtc cagaatggcc gtcagcgtgt gcacgttgcc gttgtcctta agcacgttgg
     4441 catgggggcg ggaaccgccc cggggcgtca gccccgccag cggtatgccc atgccctccc
     4501 ccctccccct ttcgcgcagg cggaaggtgg ggtccacttc atcggacatg aaaatggcct
     4561 catagaacca cggattgctg tccttggcat agggagattt ttcaatgacg aagggaaggc
     4621 tcaggcgctt ctcctcatcc atgccgcctt tgcgtttggc ttccttcagg tgggcggcca
     4681 gctcccgaat gcacgcattg tattttttcc gggcttccgg ccattcccgg ctgcggctgg
     4741 gagaggaaag aatgagccat gcggcatggg cctcccggat agccgcggcg tcatcctggt
     4801 aatgggcgta ctccatttcc tgccgctggt gcagggaaca gccggaaagg cctatggcca
     4861 gtcctaagag aaggatggaa accgttttca tgatgcgcgg tgtcaataag cagggaaggg
     4921 agggtgattc aggagagcat gtccagtgcc cggttcatgg atttgaggaa ggtctgcact
     4981 tcctcttccg tattgtacag ggcaaaggag gcgcgggtag tcccggtggt gcccagggcg
     5041 cacatgagcg gctggcagca atggtgcccg gttcttacgg caattcccat ctggtccagc
     5101 agcgtaccca ggtcatagtg gtggacgccc tccgccagga cggagaccac ggcgctgtgc
     5161 ggcacgtccg gggccagtac ggtcaggcgc agcatggcct tcaggccttc caccgccata
     5221 tccgtcagtt tctgttcatg ggcggcaatg ttgtccagac ccagcccgga gacgtagcgg
     5281 atggcggccg ccagacccac tgccccgcca atgttgggcg ttcccgcttc atatttgaac
     5341 gggatgtcgt tgtaaacggt tttttcaaag gtgacttcct tgatcatttc cccgccgccc
     5401 atccacggcg gcagtttttc cagcagctcc ctttttcccc acagcgcgcc gatgccggtg
     5461 ggtgcgtaca gcttgtggcc ggaaaaggca tacaggtcgc agcccagttc ttgaacgtcc
     5521 acattcatat gggaaacagc ctgggctccg tcaatcagca cgatggtttc ctgcctgttc
     5581 tccttggcga gcgcggccat ttccctcacg ggattaaccg ttcccagcgt attggaaacg
     5641 tgtcccacag ctacgatgcg ggtgcgtggg gaaagcatgt tccggtaagc ttccatgtcc
     5701 agggtctggc ccggcgtcag gggaaccgct ttcaggacgg cgcccgtgcg ttcgcacagc
     5761 atttgccagg gaacgatatt ggaatggtgt tcggaagcgg ttacgatgac ttcgtctccc
     5821 ggtttgacca tgccggaccc ggcgatggta tccgccgcca ggttgatgcc catcgtacag
     5881 ccggaggtga acaggacttc cgccgtttcc ggcgcgttga tgaaccgcgc caccgtttcc
     5941 cgcgattctt catgcgcttc cgtcgcgagc tggctcaggt agtgggagcc gcggtgcaca
     6001 ttggcgttct ccgtatcgta gtagtgacgg atggcgtcca gcacggccag gggtttttgc
     6061 gtagtggccg cattatccag gtaaatgaga ggcttgccgt gcacgctggt ttccagaatg
     6121 gggaactgcg ggcgaatggt tgctgtgtca agcatggcta tggtactagt gagggttctt
     6181 tgcgcaattc atcccagcct ccttccagaa tgaaaatggg cgcgcggatg ccgtattccg
     6241 gctttttcag ttcattggcc acggccgcgc tggaggtgca gtccctgctg caaaagatga
     6301 cgatgcagaa tcccttgtcc tctgccgtca tcagggcttc catggcattt gccaggagtt
     6361 cctggtaatt ggcatccgcc gggcggatgg ggtaaacggg ggcctgggtg acggtatggc
     6421 gttcaaagtc gtcctgcttt ctggcatcta tccagaggat ttttcccggc cattccttta
     6481 atacttggga gaggcacacg tgcccttcct ccagagtttc cgggttgcag ggcggcgtgc
     6541 ggaacggctg gatgacgcgc agatccagag cggcgacgcc tacggccagc agaaatacgg
     6601 ccagcaggag tttcagagcg gtggaaaagg cgtctctcat ggaaggaagg gcggatatca
     6661 gttttccttg atggccagga accccatttg gcgcttgtag cgcggcacgg cggaatccgt
     6721 gcgcacccag atggtgttga aggccggcct ggccgtggat ttgggagtga cgatgatttt
     6781 atattccctg ccttttttca cggtcacagg ctcgtagtca aaggcgtctc ccgtcaaatc
     6841 caccgtggtc aggttgacgg gcaattcttt ggagatggtg atttttatgg ttttgggcac
     6901 agcggcctct cctctggccc attccagctt gcggggttcc atccggatga tgtccgggat
     6961 ctgcgcgcgt attaccagct tgtaggcaga gccgttggcg tgcaccgtca tgtccttgtc
     7021 cacggtgccg gaaaaattgc cggttttcat tacggcgctg atgacgccgg attcccccgg
     7081 ggcatacgtc agcttgttat ccttcgctcc ggcggtggtg cagtcgcagg aaacgtggat
     7141 tttactgatg gtgaccgggg tgccggaagt gttggtgaag gggaaggatg ccgtgatgct
     7201 gtcctggtcc ggagccacct ttaccggaac caccgtttcc ttgaaggaca attcattttt
     7261 gccggaggcg aggcttccgc acaaaagcag tcctgacaga ataacgagcc gggtggtcat
     7321 gggcgtttca ggatgttttt tcttctttat tcttggcgga gaaaatgctg gcgatgaaga
     7381 aaataattgc cgctacgaag atgcaggagt ccgccacatt gaaggcgggc cagcggtagt
     7441 tgaatagggg gatggtgacg tcaatgaagt ccaccacgta accgttcatc agcttggtga
     7501 agaaaccgtg ctgttgctca tacggaatca ggaacccctg aatcagtcgg tccgtcaggt
     7561 tgccggctac gcccgccagc agcaggacat aggccagctt gagccatgcc gtgtggaaga
     7621 ggtttttccg gtaaagcacg accaggacta cgatggcgag cacgggaact gccaggaaaa
     7681 gatagctgga ccagacggtg ccgttcccca gcccgaaagc aacgcccgtg ttgtgcacgc
     7741 gggtaatgtt catggttccg tccagaaagg agatgggttc cagttccctc agtttgttca
     7801 gggcgtccac cgtgtttgga ttcagatccg ggagcctcgc cagttccgcc tccggcatgc
     7861 gtccgttcgt ggcgaagtcc agcgtgccgt ccgcattgat gtgagagaaa ccaggaaagt
     7921 ccggcaggta ggcgtactta tggagctttt cgttcaggta gttcagcggg aagcggcgga
     7981 taatccacag cttgctggcc tggtccagca catacagccc tgcgcccagc aagctccacc
     8041 agagccagcc gggaggcgtc tttttatggg gggcgggttc tttttccgtc tgggggtcca
     8101 tataaaggtc gggaaatgga gtggatgcgg ttcaggaaag aacttccgcg cagcgttcgc
     8161 atacgtcccc gccttccgcc gctggttcat accggcggca gcgcgggcac atggcatgtt
     8221 ccgtttttgc agcggaggcc gccagttccg gaccggtttt cacgtcaagg tccgcaatga
     8281 tgaaaaattc cgtggcaaac gcccggtctt ccagcagcgc cactacgtct tcgctctcat
     8341 tcacgggaac ggtcagggta atggaggcct cattattttt ggaaaatacc ttggctttca
     8401 cttgttcttc aatggctacc tggatgacgg acctgatttc ctggagacgg gaaatgacgg
     8461 aggacgcctc ctgggtatcg tattccggca tgggggcggg gaagtcctgc tcatgcacgc
     8521 tgccttcata tcccgcgcat tcccaggctt catccgccgt gaaggccagg atgggtgcca
     8581 gcagacggca cagagccttg aagataatgg tcatggccgt ttgtgtggcg cggcggcgca
     8641 cggaggagga agcgtcgcag tacaggcggt ccttggtggt gtccacgtac agggcggaca
     8701 ggtcgctggt gcagaattgg ttgatggcgc tgaacgcttt gcggaagtca taagcttcat
     8761 aggccttcag cgtttccctg atgacggcgt tcaggcgttc cagaatccag cggtccagga
     8821 tgggcatgtg ttccggggcg accgcctgcg cggcgaagtc aaatccgctg atattgccca
     8881 gcaggatgcg cagggtattg cggagacggc ggtaaggttc cgtcacctgt ttgaagaggt
     8941 cttccccgaa ggggacttca ttttgccagt ccacggaggc ggcccacagg cgcacgatgt
     9001 ctgcgccgta tttgtcgtag taataggccg cgtcaatggg tttgccgctc ttggcgtcgg
     9061 atttggaggt ttttttcccg gtatccttgt ccacgacgaa gccgtgggtc atcacggcct
     9121 tgtacggggc cttgccgcgc caggcgacgg aaagcatcag ggaactctgg aaccagccgc
     9181 ggtgctggtc cgtagcttcc aggtacaggt ccgcaggagc atccagcccg gggcggctgt
     9241 ccatgacggc catatgggaa ctgccggaat caatccagac gtccagcgta tccttgccct
     9301 tcttccaggt cttcggcagg ccgaggcgtt cgcacaggtc ttcatcggag agttcaaacc
     9361 agatattgga accgtgtttc tccaccatat ccgcaatttt gcgaaccagg gcggcatcca
     9421 gaacggcttt gccgttttca tcaaagaaga cgggcagcgg cacgccccag gtgcgctggc
     9481 gggagatgca ccagtccggc ctggcttcca ctgtgccgta aatgcggttg cggccccagg
     9541 cgggcagcca ctgcaccttg tctatttctt ccagggcctg cgggcggagc ttgtccatgg
     9601 agatgaagaa ctgttccacg gcccggaaga taataggcgt cttggaacgc cagcagtgcg
     9661 ggtactggtg gcggtattcc tccacgccga agaggttgtt gccctgttcc agcatggaaa
     9721 tgatgtcctt gttggcgtca aagacatgtt tgcccaccag ggaaggcacg cccacttcct
     9781 ccgtgtattt gccgtcatcg tccacagggg agaggacagg caacccgtat tggcgcccca
     9841 cgttatagtc gtctgcgcca tggccgggcg cgatgtgaac gatgccggtt ccggtgtccg
     9901 tggtgacaaa gtccgccagg atgattgttg acgtgcggga aaggaaggga tggcgggcct
     9961 cgcagttttc cagttcggct cccttgaatt cacggatggt ttccgcaaga gcccagcccg
    10021 ttttctgagc gaaggcatcc agaagatcct tgacgatgac gaggttttcc gtctggccgt
    10081 ttttcatgaa cctgcctgcc acgtaggtga aacgcggatg gagtgcgaga cctacgttgg
    10141 cgggcagcgt ccacggagta gtggtccaga tgaccatgga tgctttcagg cccaggggat
    10201 tgtccagaag cgggaagcgc acgaaaacgg aggaactgac cttttccttg tagtcgattt
    10261 ccgcttccgc cagcgccgta taggccccat aggaccactg gacgggtttg cggctctggt
    10321 acaccgctcc gtcttccacc agtttggcga acacgcgcag gatgttggcc tcatacgccg
    10381 gcttcatggt caggtaggga ttctcccagt cgccgaacac gcccaggcgg cggaaggaag
    10441 tgcgctgaat gtcaatgaag cctttggcga attccgcaca gcgtcggcgg atttccgccg
    10501 catccaggtc acgggccttc tgaaccacct tgaattcgat ggggaggccg tggcagtccc
    10561 agcccggaat atagggggcg gcataaccgg ccatggtttt tgatttcagg accaggtcct
    10621 tgagaatttt gttcagggcc gtacccatgt gcacgtcccc gttggcgaag ggggggccgt
    10681 catgaaggag gaaccggggt gcgccctgtg ccatcctgcg ggacaaaatt cgttcgtaca
    10741 gcccggtgtc ttcccacttt ttcaggcgta cgggttcgtt tttcgtcaga tcccctctca
    10801 tggggaaacc ggtctccggc aacaggatgg tgtctttgta attcttttcg ggcgcactca
    10861 tgcccggaaa aagtaccacc gggcccggtt ccggtcaacc ctgcggtgcg gcacacgccg
    10921 gtcctagcac attcattccc gccgtgttcc ggaggaagtg cccgctccgg agggcgcacc
    10981 cccgcgggca gaagacgagg aagaaaggaa tccctgttcc ctgagccatt gttcggcggc
    11041 ttcaggccat tgatccgtgg gatgtccgcg tttttccatg ccatacccgt ggcctcccgt
    11101 gggatagaag ttcagactgg cgggaacccc cttttcccgg caagcctgtt ccatcagcct
    11161 gctgttggcg caggaaacgc cgtcatccag agcgtggacg aggaacaccg gcggcgcctg
    11221 ctccgtcacc tgtctctccg cggaacacat ctcctccagg gcaggggatg gatgggcccc
    11281 caatattttg ttgcgcgttc ccccgtgggt agtatggggc gccatggtaa tgacgggata
    11341 aataaacatg gaaaaatccg gacgggcgga aatgtttttc agcggatttt ccgcttcttc
    11401 cggcagggtg cggttccaca gcgtagaggc catggccgcc agatggccgc cagcggaaaa
    11461 gcccatgacg ccgatgcgaa gagggtcaat tcccagggaa gaggcgtttt tccgcacggt
    11521 cctcagagcc tggcgggcgt ccagcagggg accgggaaac ttccccaggg cattgctggt
    11581 tcctgaaacg cggtatttca cgacaacccc caccatgccg tgttccgccg cccagccggc
    11641 aatgcggctc ccttcctttt caatggcctg gatggcatac ccccctcccg gaaaaataca
    11701 cagggccgtg cgcctgtcct gcggactcca tccggcggga aagtgaagct ggatttgcgc
    11761 ttcccctacg tccgtaatgt gtccggcgga ggcttgtttt tctttcaggt ccgccgcttt
    11821 gagcccaagg gaaatccatc cggagtgaaa ggcttttccc tctgagggag ggcagaaggc
    11881 gcccagcgcg gacggcttag gcggagcggc cccggctgaa gcacaggcga atgtcagaaa
    11941 aagagcggag agtaatatct gtttcttatt catggtttgt tggccagtga aatgacgagt
    12001 ttggaaaggc cctgcaccac ttctgcaaac tggggatagt ccagcttttc agccgtatcg
    12061 ctggtttcat ggtaatcgtc gcagcggagg aaggccgtat ccgtggcggc aaaggagggg
    12121 atgccttcct tcatgtatcc ccagtcgtca gaccaggaaa cgccgcgggt gtaatacggg
    12181 aacacggcgg aacgcaccgg aaagcggctc aaggcggaaa attcctccgc gcaggagcgt
    12241 gccagtttgc ggcccgtgtt ggtggagagg aaagcaacat agtcgcagcg gtccggcagg
    12301 ccggccaatc cggcaaccac ggcggattgg cgttttttat tcacccgggg ggaatagcag
    12361 cccagcgttt ccagggcaat catgccaatg attttttccc tgcccgaatg atgggagacg
    12421 gatttggcat gtactgcgct gcccatggcg ggcgtctggt aaaaaggagg ctcttcattg
    12481 gcgtaggcca ccagacaaac atcgtggagg gtggaagttg ctgcaagggc acgtgccgtt
    12541 tccagcaggg cggctacgcc ggaagcgttg tcattggctc ccggagttcc ggtgcgggaa
    12601 ggccgggcag gaccgtggtc gtgccagttg tccatgccca ctttggtatc gtaatgcgcg
    12661 ccgacgatga gcattttggg ctggggggag gttcctttct tgatggcgat gagattatat
    12721 gctgtccagt cctttacggc gccgaattct ccggagggcg ggatatgaac agcctggcgc
    12781 gtgacggcat aacccattcc ctccagtttt tgttcaatgt agcgggcgga acgctccatg
    12841 gagccggggt ggtagacgtt ccgttcaccg atggtatccg ccaggtggaa tacatgcgct
    12901 ttcaggagcg tttccgtttg gtccgtgtcc ggtatttctc cgcttccgcg gggagaaaaa
    12961 agcttggaac aggagctgag ggcgatgcag accaccacta tgagcagacc gagcctgaaa
    13021 gtcatggaaa agaaatgcat agcggaaatt gaagaggtaa cgcagaaagg ggagtcctaa
    13081 tgggaaagcg gaggaaaatc aagacgcacc gtttctccct tattcatgga caaatccttt
    13141 ttccggccct gatgcatgaa ccggaatttg cccggacgcg cagcggtagc cgtggcctgc
    13201 acccggccgt cttgccagga caagtccacc ttaagccctc cccgcgtgcg caatcccgtg
    13261 gcagacccct tcttccagtc gtctgccagg gccggagcca gcaggataac ctggaacccg
    13321 tcctgcatga tccggctctg gatcaggcat tcggccacgc cggaggaaaa gccgaaattc
    13381 ccttctatct ggaacggggg atggaggtca aaaaggttgg agttgatgta gtgcctcagc
    13441 atggtgtaca tcctgtcctc tgcggcgttc ccgtcgccaa gggcggcata aagattgatg
    13501 agccacgccg tgctccatcc cgtcctgatg ccgttgtttc ccatatccgc gtacttgcgc
    13561 cggaaatcgg ctgacttgcg tacggcctcc gcatattccg gagtattgag aacatcccat
    13621 tccgtgccgg ggaacaggcc gtaaaggtgg ctgatgtgcc ggtggccctt ctgcatttct
    13681 tcaaaaggct gcctccattc ctgcaccctt ccgtcgggac cgatggcagg ctgggggatt
    13741 ttcctgagaa attggacggc tttaagcagg gtgggagtcc ttatgcccag ctctccgcag
    13801 gcgtagatat aattgcggag agcctccctg cccagaagct ggtcatggga cgtgccgttg
    13861 gaaacatagg aaagcacgtt ggggcctgtg ccgtccggcg cgtaaaatcc ggtctccgga
    13921 gacacccccg ggccggagag gtaacggccc tcgccgtcgt cttcaaacca ggacatgatg
    13981 aaacgcgcgt tggattccag aatgggcagc gattttttaa ggtcttcccg gtctccggta
    14041 aagcggtaac tgtccaccag gtgggcgcat gcccatgccc cgttcatgag gctggccccc
    14101 cattccggat tgttgcctga aaaataggtg cgcttccagc aatccgtata gtggccgaag
    14161 cagaagccat cccgtttgat gaagcgcgca aactcctctc cgtgggggcg caggctgcgg
    14221 acaaaatcaa gataggagct ctggaattct cccagcccgg tgacatggga gggccactgg
    14281 ttcatctggc tgttgatatt caggaaatag cagcccatcc acgccgccct cagctccggg
    14341 ttccataatc cctgcaaacc gcagggaagc tgccccggcc tggtgtgggc aatcgtgcag
    14401 aagcggccga actggaaaag ctgttccaga agatccggat ccttctttcc ctgtttgacc
    14461 cgctccagcc tttcggcggt ggtcatggcg gaaaccccgg ccggggaatc ccccagatcc
    14521 acctgacagc gcgtcatgag ccgggaaaaa taatcttccg tttccgccgc cagttttttc
    14581 catcccgcct tttgcgcttt tgccaggatt cgcgcgtttt tagccgccag ggaatgcgtc
    14641 aggggagctt ccggcttccg gatattgtaa tctgtggaaa tggatgaaag caccagcact
    14701 tcccgggcgg aatcaagaat aattgttgag cctttgcggg taacggaggc tctgggcgcc
    14761 aggatgacca ccgtgttttc aaaccgggtg cctccgttgc ttccctgtcc ttccagcacc
    14821 cagccattcg gctgcgcaac aattcgggcg gacggatccg ggtgttccag attcagggaa
    14881 acgcggcatc cggacggcat cgtacaggca atgtggtagg cggcgcagtc gctggacggg
    14941 gccgccagga tttctgtggt caattccccc gtgccgaatt gggcgcgggt ggttgccttg
    15001 ccccgccgca tatccagtgt acgctgatag gaggaaggtg aaggaagccc ctggaattcc
    15061 acctgaagac gccccccctg ctggtaatcc cccgcaatgt tgccgggagg aaggatattc
    15121 ttacggaata gctggtctgc actgcggtat tttccttcct tgcaaagttc gcgcgnnnnn
    15181 nnnnnnnnnn nnnnnnnnnc ttccgcggcg ccttccctca tttggaaatt tttctttgcg
    15241 aaaatgcttc cttcattgag gacgacggtt tccttcggaa aaacgccgaa agagaggatg
    15301 cccagccttc cgttgcccgt gccgtatcct tccgtccata cccgggcggg ggtggaggcg
    15361 gtgacctgca tgggggccgg aatggcccgg agggggacgg ctgccgcgaa aaggcagagc
    15421 aggaaggaat gtttcaaggt tgcagggacg ttcatgggaa aagataatgt tgcgtataat
    15481 aaaagtaaaa gaaagctggt gtaaaaaacg ctattttttg aaccggcttc cggcgccggc
    15541 gtaaatgacc gcaaatgcaa tgcttcccac cagaaagtac agggaagcgg acaggagcac
    15601 ggagttctgg agaatgatgc tggcgagcca gagcagcatg gcgaaggcga ggcagtacat
    15661 gatcttcccc atgaaccggc acagcgcctg ttcatcatat tgctttttct gccctctgtt
    15721 catcatgctg tagcctgcga tgaggaagga acatttcccc atggaaagaa tggctccaag
    15781 aatcatgaaa cctgccatca tggcgacggg tatcggaaca ggtgcatcca taagctggta
    15841 aaatgaggga atgggagaac ccgtccgttc ggacggatgc gcgtgtagag cggcgtttaa
    15901 ctgaatacgt tggggaaact gtgttttttt cagcagcaga aaaatgtctg cttttcctgc
    15961 cggaaacttc cgccgggcgc ggcatcaaaa caggaagcag caggaacaga accaggcgat
    16021 gatgagcagg ccgcaggtaa attccagcag cagaccggca agcatcccaa gcgccgctcc
    16081 ggacccggct ctgaaagcgt tcatgatatc ctttctggca aagataagtt ccgccagcaa
    16141 cgcccccagg aacggcatga acaaaaggcc cgtaatgggg gagaacagga ggctccccgc
    16201 gacggcgccg atgatggctc cccagatgcc ggcggcagtg ctgccgaatt ttttggcgcc
    16261 catgcctccg gaaagcttgt caatgaccat gcctcccgcc accagcagcg ccaggacggt
    16321 ccattcccac cacgccaggt tttctctgcc catgactccc tgccagacgc acccggccgc
    16381 aatgatcata atgccgggaa gcatggggat gagcgtgccg atcagcccca tgccgaataa
    16441 cagcagggtg acgcaccaga cgagggtttc cgaaaggaga tggggcatgc tttccttttt
    16501 atcaggaagc cggacaggtg gcaaggtttc tgcccggctt gcggatgacg aaaaaaggaa
    16561 aaaaggggga gaaattatta ttgcggttgc tgcgggcgtg gtatggaatg gcgcctatga
    16621 cttccaccgg tgtttatcta tgcggttgca tcatgttctt ttgcgttttt tctcaggggg
    16681 ccacgtcttc tccgccagcc gtttccgcag cggattcctc cgctgtacgc gaactggccc
    16741 attccctgaa ggcccgtgtg ggcatggctg cggaaatgct ggatacgggg gaaaccgtca
    16801 tggtggggga tgaggcggcc tatcccatgc agagcgtcgt caaattcgtg ctggccttgt
    16861 ccgtcttgaa gcgggtggac cagggcgcca tgaatctgga acagatcatc cgcatacgtc
    16921 cggaacaact ggtgaaagat acctggagcc ccctccggga gcgtttcccg caaggcggcg
    16981 atttctctct gaaggaactg ctgagagtga ctgtccagga aagcgacaac aatacctgcg
    17041 acctcctgtt tggccttatt ggagggccgc aggccgtgca gaaggatttg aaggaatggg
    17101 gaattgacgg cattaatgtc cgttttacgg aagaagaaat acaccggaat catgatttgc
    17161 aatatgtcaa ctcaagccgt ccgtcggcca tgaactccct tctccgggcg tttgacgaag
    17221 gtaaaatttt gaaaaaaggc acacagagtg ttctttggaa catcatggcg ggatgttcta
    17281 caggtcctga acgtctgaaa gggcagctgc ctcgggatta cgtggtggcg cataagacgg
    17341 gatccggatt cacgctgccg gggggtggcg ttaccgcccg caatgacgtc ggcatcattg
    17401 ttcttcccaa cggccggaaa atggcggttt ccgtgttcat cagggattca aaggattctg
    17461 aatccgcttg tgaccgggtg atcgcctcca tggcccgctg gctgtgcctg gaatggctgg
    17521 gtgccggagg caggtaaacg atctccttcc ccggaatatg ttttaggctt ggaaagggga
    17581 gcgggatcag gcaaggtgtg ccgcgttatt catgaagaac ctattgagcc ggtacatcag
    17641 cctgagtctg gcgctgcggt atttgaaccc gttgcggact ttcttttcca ttattaccct
    17701 gatttgcctg ctgggcgtat ctctgggggt aatggtgctc attgtcgtgc tgtccgtcat
    17761 gggaggcctc cagaaggaaa ttcaggggaa tctgtttgcg cactccccgc acgtccaggt
    17821 atgctaccgg aacgacttcg gcgtgaggga agtgattccg gactggatgg atttggggga
    17881 gaaactcagg cacgttcccg gcgtccagtc cacctacgcc ctgatagaag actatgcgct
    17941 ggtggatgtg caggggcggc agaggccctg ctttttccgg gccattgata cggaaaacgc
    18001 cgcccagttg gaggatttga agcgtttggt agtagctggc gatgcggaac tggacatggg
    18061 ggaaaaggct gtggtttcct ccatcgtggc ggagaatatg ggattgaatg tgggagatgt
    18121 tgtccgggtt tatacgaccc gcaattttca ggaaatttcc cacgcctacc agcagacgga
    18181 gctgccgatg ctggcggaaa aaaacgcgcg ggaactcaag gatttgaagg agtggggaaa
    18241 gaacctgaag acggagggag agcgtgaaat ggccggcaga gcctccgtgg acagggcgtt
    18301 ttccctgctt aacgggctgt tgtccgtccc gcgcagggat gcggaacgtt ccagcctgat
    18361 ggatctgctt gccgtgctga acgacgggga aattgcggac aacggcaggg tggcctttcc
    18421 gcaaggaacg cggaaggaat gggaaaccgt tttgagcggt ttcaaggccg gggcaagaaa
    18481 tgaggcggat atggaatgct tccgaaaaat caaggagctg gtgatgccga aagacctgga
    18541 ggtgatcgga atttaccggg cctcccagca tacgcccagc ccggatcttt tcattccgct
    18601 ggtcatcggc caggaattgc tgggttatga ggatgacgtg gtgcaggccg tggccctgcg
    18661 cgtggaagac ccttaccatg tggaaacgat gctggccccc gtgatgcagg cgctggaaaa
    18721 ggagaagcct tcttccgcat ggacgctgga aacctggcat gaccgcttta acgcgtggtt
    18781 tgaattgatg cagaaggagc gcatgatgat gagctttgtc ctgtccttca tctctctgat
    18841 ttccgctttt tgcatcatgg cggtgatgtt taccgtttcc atccagagga aaaaggaaat
    18901 cgccgtgatg aaggctttgg gagccacgcc gttccaggtg gtgcgcgttt tcctgtggca
    18961 gggcgtcatc atcggttttg tgggagctct cctgggtgta gggctggggc ttctggtgct
    19021 ggaataccgc atgcagattc agggttttct tgcgggaata ggctttgacc cgttccctgt
    19081 ggccttccac ggcacggcga atatcccggt ggtgattgac tgggcggagc tggcatggca
    19141 ggcggtgaaa gcttttgtca tggtcgtggt ggcatccatc attcctgccc tgatcacggc
    19201 gcgccaggat ccggcccgct ccctgcgcag tatgtaacag gaatcgccta tggatattta
    19261 ctggattcag gatcatgaac ggaaggggcc tctgccggaa gtggaggtca tttccatgct
    19321 ggaggcgggc ctcattccgg aaaacgcccg ggcgtggcat gccggatgcc cggagtgggt
    19381 atgcatccgt gaccttccgg cattgaaagg agcaggtgcc gtcagcagag aagaagggga
    19441 acggcggaac gggagggaag agaggctgga aaacgcaggg ggagaagatg tcccggcgct
    19501 ttccgcagat gcggattgcc tgccggaaga aggaggggag gacgcggagg agggagtgcc
    19561 gctggtggtt ccgtatgcgt acgtccgttt tcttggcaga atggctgatg taatgatgca
    19621 catgacgctg tacctggctg tgctcagggt atccggcgct gctttcaatc ccggttttct
    19681 tcctggttcc tatgaagctc tgctttacct ctgcctcccg atggttctca tggaaactgc
    19741 atttcttgga acattgggca caaccccggg aaaagccatg ctgggcgttt ccgtcaggga
    19801 ctaccgggga agacgccttt ctttccccat ggcattccgg cgctccctgt tcgtcatggt
    19861 gcttggtctg gggtgttttg ctccttccct gatgctgctg gccctgtttt tttcctggtg
    19921 gtgggtgcgc cgtttcggtt ttactccctg ggacaggaag ctggggacta cggatgtgct
    19981 gaacggctcc ctcacgctcc gcaaagtggt gatgacgttg gtgctgatca ttctgtgcct
    20041 tcaactcatc tacgttctgc tgattccctg gctgcctgag atggaggctt atgcggccgc
    20101 ttccggccaa atgtgataaa aagacggccc tgcggggttt aggcttttaa tcacctgaat
    20161 ttgtttcata cttgtttcag ccatgccaga aatacaccca acggcagtag tgcatcctgc
    20221 tgcagagatc gcggatgatg ttaaaatcgg tcccttttgc gttgtcgggg aacatgtcaa
    20281 actggggccc ggctgcgtgc tgcacagcca tgtggttata gacggtcctt cttccttcgg
    20341 cagcggcaac gaatttttcc cgttttccgt catcggcctg aaaagccagg atttgaaata
    20401 caagggagag ccgacttatc tggaagtggg ggataacaac gtcttccgtg aaaacgccac
    20461 catcaaccgg gctacggaca ttggcggcgc gacgcggata gggaataaca accttttcct
    20521 ggtttcctgc catgccgggc acgattgcca gatcggcaat catgtgattt tctccgggtt
    20581 cgctaccgcc gccggccatg ttacggtggg ggattacgcc atcctggcgg gatgttgcgc
    20641 cgtacaccag tttgtgagca tcggggaaca tgccatggtg ggagcaatgg cccgcgtgag
    20701 ccaggatgtc ctgccgtaca cgatcgtgga gggccacccg gccgtaacgc gttccgttaa
    20761 ctccatcggc atgcagaggc gcggcttttc cgaagaagac ctgaaagccg tgcgcatgtg
    20821 ctataaaaag cttttcgtca ataaaaagct gaccgttcat gaagctctgg aggaactgcg
    20881 gcattcgggg tatgcggaaa atgcctgcct gaggagaatc atccaattcg tggaaacttc
    20941 cgaacggggg ttctgccatt gaatccatga aaacgggttt tgtctttgac ctggacggga
    21001 cgctggtgga ctccattccc ggtatcgccc gcggcctgaa tctggccttg aaagcgctgg
    21061 ggtatccgga gcattccgtg gacgccatcc gggaaatggt gggcaagggg gcccgggagc
    21121 tttgcctggc ttccctgaaa ggctatttca acggaaacgt gccggaggaa gcttttgaag
    21181 cggtgcaccg gggattcatg cgtgaatacc cgcacacctg gcaggagggc acggttccgt
    21241 accccggcat ccgggaaatg ctgttgtccc tggccggaga aggccatcct ctgggcgttt
    21301 tatccaacaa gccccatgcg gttacggtgc cgctggtgcg ccatatcctg cctgaaattc
    21361 ccttccggga ggtgatgggt ttttccgaac gctttccgcg caagccggaa cctgactccc
    21421 tgctgtccat cgtccgttcc tggggcaggg agccggcgca ggtgtgcatg gtgggggact
    21481 ccgcccatga cggcaacacg gccgtgaacg ccggaacacg gcttattctg gtggggtggg
    21541 gctacagttc ccgttccgct ctggatgcct tccgggtgcc ggtgtgcgcc gccgcggggg
    21601 aattatctgc ccggctgcat gaggaaagca gcctgccgtt tcccgttgcc gcgcatagga
    21661 attgttgaaa attccgggaa gtgcagggcc gtcggaggct aaattcctct acatctccaa
    21721 gatgctttca agcgcaggaa agcgtttgcg ggggatgcct tagagctgta ttttcccggc
    21781 tcggaaatgg gcaggaaggg aaaaagacgt attgttgccg ctctttcagg acgtagtatc
    21841 cggaacatcc gcagatttgg cctgttcggt aatgtcttcc agggagcggg gaagttcctt
    21901 cgtggaggtg acgcccagat ttttcaactt ctgggctcgg gggatgaacc ggtgttcaaa
    21961 agaggaaacg gttttgttgt actgggctac cgcctgattc aggctcttgc ccagagagga
    22021 aaaatgaccg gaaaggatag agcaggtatt gtacaggtcc gctcccgctt ccgaaatttt
    22081 cttggcatta tccgccagct gctcctgctt ccatccgtaa gcgacggctt tcagcagggc
    22141 aatcagggtg gatggggtgg aaagaatgac tttattctcc gcgccgaatt caatcaggga
    22201 gggatctgct tccagagccg cctggaaaaa ggattccccg ggcaggaaca taatgacgaa
    22261 ttcggggcat ggggaaaact gggcaaagta attcttggcg cttaattgct gcaaatgcct
    22321 cttgacgtcc gcggcatggc gggccatcca ctggctgcgc tcttccggat tctccgtctg
    22381 cccggcattc aggtaggatg ccatgggcgc tttcgcgtca atgatgatgg aacggcctcc
    22441 aggaaggtga ataaccatgt ccggccgcac ccgttcctcc tccctggaac gggtttcctg
    22501 agtcgtgaaa tcgcagtact ccaccattcc cgccagttcc accacgcggc gcagttggag
    22561 ttctccccat tggccgcgaa cgctgttgtg gtgcagagcc tgggaaagcc tggcggtttc
    22621 atttttcagg gcttcattgc cggaaaggat atagcggatt tgcgttttca ggtccgtata
    22681 ggcgcccacg cgttcctttt ccatctctcc cagccgggac tggaccagtt tcagggattc
    22741 gctcaccggc ttcagcatgg actcgatggc ctgtttgcgg gaatccaggt catggttggc
    22801 ctgctggcgc tggctttgca gccgcgtttc cgccaggttc agaaactgtt tctccgagtt
    22861 ccggagaact tccgcgctga tgccggcgaa ggaatctttc agcacctctt ccgcccggcg
    22921 gagcatctgg gttctgtcct ccgcccgttc ctgccaggag cgggattccg cttcgaggcg
    22981 gacggagcgg tcccggtacc gcagcagcag aaccagcagc gccacgcagg cgatcagcag
    23041 caaaaaggcc aggaaccagg gaaaaaatgg ggaaatcata aggaaaaaat aagcaatcag
    23101 atgggggtgg cgtaggggat gccgtccgtt tcttccacgg gttcggccag ggggatgtta
    23161 tcgtcgctgc cgtccagcgg ttccgcatag ggaatgctct cttccgaatc cggttccgcc
    23221 atgggaatgt gccgttcaca catgttggcg ggggcctggt agcctgcgga ggtttcaaaa
    23281 tacgcagtct tggcgtactg gcagccggaa tgggccagtt gattggattc ccggcagacg
    23341 agtacggcct gcccttccga accgggacgc gtacggatgg cgttggcggg atagccttcc
    23401 ttctgggcgg caagcataat gtccacccag acgggcaggg ccagggtgcc tccgtagcct
    23461 ttttccagaa ttttggtgga ggaatcaaac cccacccaga cactgcaggt gaggttggac
    23521 gtatatccgg cgaaccatgc gttcgtataa ttattcgtgg ttccggtttt gccgccgcag
    23581 ggggatttaa agcccagcgt ggtaattttg cctgccgtgc ctccgggctt gcagacctgc
    23641 tggaggatgg aagaggtaat gttcgccgcc cgctgggaat agacggtgcg cttgctcttg
    23701 gtgatggcga agcgcggctg gccctgggaa tccgtaatat ggtcaatgat gtacggcgtg
    23761 gggcgtacgc ccccgttggc aaaaacgctg taggcgctgg caatgtccag aggggacgct
    23821 tcccacgtgc ccagataaag ggccggggtg cgggaaatat ttccgtggaa gcccgccagt
    23881 tgtgcggatt gaatgacatt ggacaaaccg gcgcggttgc cgatgcgcac ggacatggtg
    23941 ttgcgggaaa ggatgaggcc gaaggaggcc ggcttcatgc cccggtacgt tccgtccgaa
    24001 ttgcgggggg accagttggc ggctccgcgt atttccccat aggcaatcct gtcatccgaa
    24061 atgcgggtat ccgcagaatt gccctgctgg aaaaacgtgg cgtaaatgaa aggtttgaac
    24121 agggagccgg tctgacgcct ggactgaatg gcgcggttca gcttggattc ttctgcgtcc
    24181 cggccgccga cgacggccag aagagccccc gtggcattgt ccatgactac ggcggcggat
    24241 tggatgtagt tgggcatcgg cggctgcgtc ttttccttct gttccgcagg gagcgccttc
    24301 caggcggcca gggaggcctc atattccgcc ttggtggtca gcttgttttt cagaattccc
    24361 ctcttctccc tcttggcctg cagggaagcc aggtgcttct tgaaatcctt gcgggcttcc
    24421 agcgcgtcca tgtgcttatt gatggcgtcc agcgtggcgt tctgcagatc aaggtccaat
    24481 gtggtatgca ccaccagccc tcccaggcgg atgtcttctt cctccagaat ggcgtccagc
    24541 tcccggcgca cgagacccat ggcgtaattt tcctcagacc gggactgggg cgtccgggtg
    24601 gcgataggct ccgccagggc cgcctgatac tgcacttccg taatcttgcc ttcgtttttc
    24661 agcaggccaa ggacaatatc gcgcactttt tttgcttcct ccggatgttt gatgggggaa
    24721 aaatcatttg ggccgtaaat aatgccggcc agggtggcgc attccgccag ggataaatcc
    24781 cggggttcct tgtcgtaata tccccgcgcc gccgccgcta tccccaagaa ggtgtgcccc
    24841 cagaaaatac ggttgatgta agtctccaga atttcgtcct tcgtataagt ggcttcaatg
    24901 cgtttcgcca gggccacttc cgtcagtttg gaatggaggt tgcgctcccg gtggttatat
    24961 gtaattttag caagctgcat cgtcagggtg gaagccccct gcgtggctct cttgtgcttg
    25021 agcacctgcg caaaggcgcg gcccacgcct atccagtcca cgccgccatg gtcgtaaaag
    25081 cggttgtctt cgcgcaggag cagggcatca ataaaaacgg gaggaacttc ctgaagggat
    25141 acccgcttgc ggttttcccc gtgcagggtt ccgatggtcc ggtttttacg gtccagaatc
    25201 accgtgcgtt ccgggatgtt gctctttacc tcggaaatgt catagcggtt ggaaatggtg
    25261 ccgtaaagaa acaccaggat ggccccgatc acaatgccga cgaagcagca tcccagaagg
    25321 atgcgcaggg gccagcaaat gaaccagcgg atattccgcg tcagggagcc gatgaagcgg
    25381 aacggcatca gcaggacaaa cagcagggct ccgaaaatcg tgaaacgcgg cctgcgcatg
    25441 gttcctgcgc gtgcggggcg gttttcttct tctctttccg gattgtcttc gtactcttcc
    25501 ttcggagacg ggcgggaggt ccgtcgctgg ggcggcgtgt cataccggga aggaccgttg
    25561 cgggaattcc ccgttctgcg ggtagggccg ggaatgacgg ccggatcagg agcttcctgc
    25621 ggcaacccgg gataggatgg acggggaagg ggcctttttt tgcgttccgt ctgggaacgg
    25681 gaagacattt ctccatcttg gatgcgttcc ctccaggagg cggcatttcg tttccttgat
    25741 ttgtcgttta aacccatccg tgggccggag tttatcataa ataaccgaaa tggtctaagc
    25801 aaaagatggg gcgccatgtc gcaatccata gacaaaattc tgccgttttg aacgtttata
    25861 cagtagggta tgtcctatca ttcgattatc ggtaatttca tgatatcact attgagaatg
    25921 acgagttttt gttgcttggt cgttgggggg cttgtgtccc tgctgtcagg gggggcgagc
    25981 cttgcgggta atgtttcttc catggcggat ttgcagaaac atgtgcatga ggtggcctcc
    26041 agggttacgg ctgctacggt ggctctggtt tccgacgggg gagagacggg cagcggcgtc
    26101 attgtctctc cgcagggcct gattttgacg gcggcccacg tcgtgggcgg cgatgaaatc
    26161 atgcgtgtgg tctttgccga cgggcgcgtg gtaaagggac gggtgctggg cgccaacttc
    26221 acgcgggatg ccgccatggt gcaaatcatg gacggaggca actatcctca tgtggaactg
    26281 ggggaatccg acggtcttca tgtgggggat tttgtggtgg ctctgggcca ttccaaggga
    26341 tttgacccgg aacgccgcgc gcccatccgc atgggaagat tgtgcacgga cggaaagcag
    26401 cgcttcctta tttcggaatg tacgctgatt ggcggagatt ccggcggtcc cctctttgac
    26461 ctgtccggca agttggttgg tatccattcc tccattggcc ctatgttgaa aatcaacaac
    26521 catgtccctg tttccgtttt caggagagac tgggacaaac tgctctccgg caggcattgg
    26581 ggccagctgg gccttcatcc catggcggac cccgaatctc ctgtgctcgg gtttgccatg
    26641 atggatgtgc tgggcgtgga cggcgtggtg gtggaagacg ttgtggtgaa ttctccggca
    26701 gataccgccg gcatccagcc gggggacgtc attacccaca tggacagccg gagcctgcgt
    26761 tccgtgcggg atatgctccg tgaactgggc aggcaccgtc cgggggaaac cgttccattg
    26821 gtggtggtga ggaaaggtac ggcttacaag gcggatctta cattcggcag gcgcggcgac
    26881 ctgatgtccg gcctgaaata ccaggaacaa actcaaggat gatgatgaaa tatttatggg
    26941 cggcatttgc ggcggcttcc gcgtatgccc aggaaatagc ttccccgctg cctccggagc
    27001 agatgattgc cccggaggac aaaagcgtgc tggacagcca ggccaaggag attttccggg
    27061 aatgggacaa ggtggctgtt ccggtggggc agtccgtagt ggctctggtt gccggcaatg
    27121 cccaggtggc tttgggaaca gtggtgggga agggaaaagt cctcaccaag ctgagcgatc
    27181 tccagaagga acggcgtccg gtcatgctgg tggattcttc cgggcgggtt tatgacgcca
    27241 aggtgctctt tgcccttccg gagcatgatc tgctgatgat ggatgtgccg gggcttcccg
    27301 ctccccccat tgacctggat tcctatgtgc aggcgaagga aggggacgtg attgcagccg
    27361 tgtcgcccac ggggcatgtg agcgacttcg gagtcgtttc cgtggctcag cgcagcttgc
    27421 gggcggatga ccagccttat ctcggcatcg tttccgatcc ccgctgggac ggggaggggg
    27481 ttatgatagg cggcgtggaa gccggaagcg gcgcgcaccg cagcggattg acggccggcg
    27541 atgtgctgat gaagctgaat ggaaagccgg tggacggaat gtactccatc cgtgccgcca
    27601 tggtcggggt gcgtcccggt gagacggttc ccgtggaagt aaggcggcgg aaccaggtga
    27661 tagaaggaaa actcctcacg gggcccaggc ccagggtgat gaaattcccg caaaagcgtc
    27721 tcgacatgat gaactccatg ggcaaccgga tgagcttgaa aagggatgaa ttccctttgg
    27781 ttatccagtc ggacatgacc ttgttcccgg aacgggccgg atgcccggtc attgacgtca
    27841 acggaaaatt tgttgggctg gctctgagcc gggccggacg cacggagacc tatatcctgc
    27901 cttcctggat atgcagggaa ctggtggaag gagtgctccc gcaggtgcag caataccggg
    27961 cgggacgcgg ggaaaacatt ccggaggccc agcctgtgga tgattcctat gacgcccggc
    28021 gtctggagga aaaccggcga aaggtggagg ataagatgag ccgtcagggc ctggtgccca
    28081 aagtttatta agcctgcccg gatgccggaa ggcggcaagt gaactttttc ggcaaaccgc
    28141 atggcttcga gacctctttc tcagcctgcc gcattggagc cgcgggaaac agggcggacg
    28201 ttgttcccgc gccggaaaaa tgtccggagt ggtttattcc ctcaatccat ggcgtcagga
    28261 gaagaaaaat aaaaaaacgg gcagcaatgc ccgtttttct gaatcatccg gatatactcc
    28321 agagcagctt ccggcttccc ggatatcgga agaaaattta cttgtcagag gaagccgccc
    28381 ccggcctttc cttgaaaatc atcagttccg tcaaaacgac ggtgcggccg gtggaatccg
    28441 gggaaataat catgaccact tttcgtcccg ggtaggtcgt cagcatcatg cctctgtcct
    28501 tcaccttgac gactttgccg ttctttaaac gggtcatgta cgtcaaattg gcgggggtgg
    28561 tgcgggaaat gttgctgtct gtagagttct tgccgaaaat gtaagtggat ttgctgggca
    28621 gttcgatagc gctcttttct cccgagggag gtttgggaag ttccaaagtg aacgtgcgtc
    28681 cggtcaggtt ctggatgtaa acgcagttca gcggcagttc cgactcatta ataaaatcca
    28741 gcgtcaattc ccctttgctg tttttgccga ccagagccag ggttttggag ccaagatcgg
    28801 gcggcagggg cttgctgacg agtttattgg tcggcatgcc tttctggtcc aggcccccga
    28861 acagggtaat gatcttgctc ctggggtaaa cgctgcggcg tcccggaagg gcggcgcgga
    28921 tttccacttt ttccagcctt ttggaatcaa taggcatgta aatgggagaa ggaagagaca
    28981 tgggctcctt cagaacgaac cggatgccgt tgacggcggc tggcggcagg gacttgtcca
    29041 cagtatcggc cgccggagca tttgctgccg gttgggccgg ttccggcctg gagggggtgc
    29101 ggctttgtcc ttgggcgaag gaaatgcttc cgaaaaggca ggtaaaggcc aggatgggta
    29161 aaatacgcat atattctggg ttctggggaa tggaaaaagt tttgatacat tgcataaccc
    29221 tttggcggat aaccgcaagg aggaaatgcg gaagggcatg ggcatcggtc cgcgcagcgg
    29281 agcgcgatgg caggcccctg acgggctttt atcccttgtg aagtacatga atgggagtaa
    29341 aggtgcaagc gaaaaatagg ggattaaggg ataaaaatcc ctga
//
LOCUS       DAWEMX010000012        28619 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_679_length_28619_cov_13.090008, whole genome shotgun sequence.
ACCESSION   DAWEMX010000012 DAWEMX010000000
VERSION     DAWEMX010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 28619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28619
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_28619_cov_13.090008"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9963..9972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15373..15382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26207..26216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6009 a         7409 c         8615 g         6556 t
ORIGIN      
        1 gggcggctgc catttctacg gtttccgccg tgtttctgaa ccaggtgctg cggacgtaaa
       61 cgccgccgat gacgagaagg tgaagaagaa gaaggacgac gagccagcgc acgacggtgc
      121 tgctgggcgc gtcatcttca aattcggaga cgcgggcgcg gtatttgctg agtttggcgc
      181 ggagtacggt gcccattttg cgtttcggac gggtgcgtcc ggtcggattg tgatcaggtg
      241 tcttggtcgt tttcatgatg gtcgttttct ggtgaattgt caggaaaggg ggggaagggc
      301 gttcaccgcg tccctgaagg cctggccgcg ctgaacataa ccggtgaaca tgtcaaagga
      361 agaagtgccg ggagagaaca ggacggtgtc tcccggccgg gcgagagagc gtgctttcgc
      421 cacgcaggcc tctacatctg cggctttttc cgtggggacc gcaggggaaa aagcgtgctg
      481 gagctggtcc gcaatctggc cgaataccac gcaggcgcgc actttttctt tcaacatggg
      541 ccgcaggggt acgtaatcca gccccttatc cttgcctccg gcaatcagga tgacggggga
      601 atgttgtgat ttcagagccg cttccagcgc atgcagattc gtggctttgg aatcgttcag
      661 ccacagcacg ccgtccagtg tgcggacggt ttcgcagcgg tggccggggg gtgcgaactc
      721 cttcagtact tctgcggctg tttctgcggg aatgcccagg tggcggcatg ccaggacggc
      781 ggccatggcg ttttccgcat tatgacgctg gttcagggcg gtcccttcca ggttcagcag
      841 gggagcgccg ccctccagaa tcaacggttc gcggtaataa agatcagccg ccggatcttc
      901 cgggctgaag gtgagtacgg aggctttcag gtgcggaagc cgttccccgg agcgaacgac
      961 ggccaggtct ttttccgtct ggttgtcgaa gatatggagt ttggcgcggt ggtaatcctc
     1021 cacggatttg tagcggtcca tgtggtccgg cgcaaaattg agccatacgg ccacatccgg
     1081 atgaaaatcg cggatggttt ccaactggaa ggagctgacc tccagcgcca gcacgtccgg
     1141 cacggaatcc cggagcagga tttccgccac cggcacgccg taattgccgc aggcaacggc
     1201 ggtctttccg gcgcgcagca aaatcttttc cactagggat gtggtggtgg ttttgccgtt
     1261 ggttccggtg atggcgatga tgcggccatg gtaaaagcgg catgccagct ccatttcacc
     1321 gatggtttcc acgccttccc gtccaaagga tttgacaaag gggctatccg tctcaatgcc
     1381 gggggagatg accaccatat ccgcatgaaa ggcgcgtccg tcttcctccg tggcggcggt
     1441 atgcagggga atgtcttccg gccatccgtc aatggaggag gaagcgtcaa acacgcatac
     1501 ccgtgcgcca tgcttcatgg cgaggcgtgc cgcggcgcgt ccgcttcttc ccgctcccag
     1561 aacggcgatg ttgaggttat tgagctgcat gttcgtggtt tgtcaggcgg tggtaatcag
     1621 gaaaaggccc aggaaagcca gcaggaggct gatcatccag aagcgggcaa tcacctgcgt
     1681 ttccttccag cctttgagtt caaaatggtg gtggatggga gccatggcga agatgcgttt
     1741 gccgtggcgc agcttgtagc tgcctacctg cagcacgaca gacgcggcct ccatgacgaa
     1801 gacgcccccg atgatgataa agagaagctc ctgcgccgtg cagacggcag ccatgccgaa
     1861 cgctccgccc agggccaggg agccggtgtc tcccataaat accctggccg ggtagcaatt
     1921 gtgccacagg aagcccaggc aggcccctac cagggccatc atgaagacgc tgatttctcc
     1981 cgcgccgggg tggaaaggaa tatccaggga atcagccgtc agccagcttc cgcagaggac
     2041 ggcaatgatg gcgtaagtga tgcccgtggc gacggagcag ccggaggcga ggccgtccag
     2101 gccgtccgtc aggttcaccg cattggaggc ggacatgacc accacgacgc cgaaaggaat
     2161 gtaaacgaac catggcaggt tcacctggcc atagaacgga atgaacaggg aggatatgta
     2221 gtcgtgaagt tccacgcggg gactgccttc cgggtacaga tacaggaagg tgacgccggc
     2281 caggccggcc aggaactgga cgaggagttt tttacgtgcg gaaatgccgt ccgaggtttt
     2341 tttcgccacc ttcaggtaat cgtcccggaa tcccagcagg cccagcgcca gggtgacgat
     2401 caggcaggca atgataaagg gattgcccat tctggcgcac aacagggtag ccgccgtcat
     2461 ggaccctacg atcagcacgc cgcccatggt gggcgttccc gccttggccc cgtggagttc
     2521 cgccagcttg tgcacctctt ccgccgtcct gatgggctgg ccgattttca gggaaatcag
     2581 cgcccggatg acccgcgggg cgaatatcat ggtcagggca aaggaaacca ggcaggccag
     2641 gagggccctc cctgccggtt ctgcaaatgc ccccgagggg ctgaactgtt caataacgga
     2701 atgcataaaa tttactgtgg cggaaaggtg aggttcatga cttgctccat ccgggcgagg
     2761 cggctgcctt tgaagaggac gatgtctccc ggcgccgtat ggctgcggag ccattctgcc
     2821 gcttcctccg cagagccgaa atgcaggctt tgagtgaagg cgttcggaga aatagcgtcc
     2881 gtaatccggg cggcatccgt tcctacgctg acgacgcagt caagacggag cccttccgcc
     2941 gtgcggccca ccagaacgtg cccttcctcg gaaaacgcgc ccagttcccc cattctgccc
     3001 agcaccgcaa agcgtctgcc ggtgcaggaa agccccgcta cggtatgcag ggcggcctgc
     3061 atggaatccg ggttggcatt gtaggtgtca tccactacca gaatgccgtt gatccgggtg
     3121 cagtgaagcc tgccgcccgt gagccgcgcc tgattgaggc cggaggcgat ttgctctttc
     3181 gcaagacctg ccgcccagcc ggcggcggcg gccagaagcg cattggttac catgtgccgg
     3241 ccgtggacgg gcaattccac ctcttcccgg cagaaatccg ggatggaaag aatgaagcgc
     3301 gtcccgttct cggtagagac gggattttcc gcgcgaacgg ttccggcgcc aatgccgcag
     3361 tcaatacagg cggcgcgggt ggattgacgg atcatatccg cataatcgca gtccgccgga
     3421 aaaatcatga agccgtcttc cggcaggcag cgtgccaacg tgcatttttc ccgggcgatg
     3481 ttttcccgcg aacccagata ttccatgtgg gaggtgccga tgctggtgat gatgccgatt
     3541 ttcgggcgcg tcatttcaca caggggagcc agttccccgg agtggttcat gcccatttcc
     3601 catacggcgg cttcgtcgga cggggtggcc cggaggatgc tgagggggac gccgatgtgg
     3661 ttgttcaggt tgccctgcgt ggcgatggtg cggaacccct gtgaaagaac ggaggccgtc
     3721 agatccttgg tagaggtttt tccgttggag ccggtcaggc cgatgacggt gagggtaagc
     3781 tctgaacgcc accaggcggc cagcttctga agggcttcca gcgtgtcttc caccagaatg
     3841 acggcgcagg aggggtccat gccttcctcc acacggctga cgacgacgac ggccgcagta
     3901 cgggatgcct ccggtgcaaa caggttgccg tcaaattttt ctccccccag cgcgaagaaa
     3961 acggcatggg gcggcaaatg gcggctgtcc gtgcacacgc cgccggaagc gaccctgtcg
     4021 aaggggccgc tgaccagttt tccgccgatg acattgctga tgccgtgtgc cgtgagggaa
     4081 atcatgaaag aaagacggaa taacgtgtta tgcctggggg aattcttccc gttccttgat
     4141 gaacatgttg cggcgcactt ccttggcgtc gctgaaatca atgcggccgg tggaaagttc
     4201 ctggtaattt tcatggccct tgcccgcaat gaggacgatg tcccccagac gggcctgctc
     4261 gatggccgtg gcaatggctc tggcccggtc cgggatgatg gcgtatctgc cttccggcat
     4321 gcccgccgca atctggctga tgatggagag gggttcctcg cttctgggat tgtcggaggt
     4381 gacgatgcag gcgtctgaca ggcgcgcggc tgcggctccc atcagggggc gttttcccct
     4441 gtcccggtcc ccgccgcaac cgaacacggt aatcaggcgg cgggagcaca gttccttgag
     4501 cgtcttgcag acgttttcca gggcgtccgg cgtgtgggcg tagtcaatga agatctgggc
     4561 gcctccgggg tgggtgaaca gttccagcct gccggggacc tgcggaatat tctgaaggtt
     4621 cgcaatggcg tcccgcacgg ggatgccggc gcaaatgacg gcggccagcg cggccaggct
     4681 gttgtacatg ttgaacttgc cgatcagggg gacacggacc aggtagcttt tccccttgta
     4741 ttccaattcg tactcgctgc ctttggcggt ggcgtggtgc accagcatgc ggaaatccgc
     4801 ccccagggcg gacccgtagg tctttacggt catgcggccg gaaaacattt ccgccagccg
     4861 gcgaccgtaa gtgtcgtcga tgttgatgac ggccacgggt tttcgccggg atctggtgtc
     4921 ctgtgccatc tgttcgaaca atttcgcctt ggcctggaaa taattctcca tggtgtgatg
     4981 gtagtccagg tggtcctggg tcagattggt gaaaatgccc acgttgaaat tgatgccggc
     5041 cacgcgttcc tggtccagcg cgtgggaaga gacctccatg gagacgccgc ggcagccgtt
     5101 ttcatacatt tccttcagca ggtgttccag ttccagcggt ccgggcgtgg tatgggtgga
     5161 aggagtgatt tcctccccgt tgtcataggc gatggtgccg atcaatccag cgcgcagcca
     5221 ggactgcttg agcagggaat gggcgatgta ggccgttgtg gtttttccgt tcgtccccgt
     5281 cacgccgatc atggccatgt ggcgggaagg gcgtccgttc caggccgcgc ccatcaggct
     5341 gacggccagg cgggagtcgt tcacctccac ccagctgact ccggcttccc tggcttcctg
     5401 cgtgcagggc atttccgcga cgacggcgca tgctccgcgg cgaatggcct ccgggatgaa
     5461 ttcatggccg tccgcccggg ttcccttcag ggcaatgtag caggaaccgg gcagaatgcg
     5521 ccggctgtcg cattccagat gggagatttt cacctggggg gagccggtga gcgtatggga
     5581 gggaaggtct ttgagtaagg taagtagctt catgacggga ttatctgcgc ggcggtttgg
     5641 atggggtgga agaggcctgg gcacgggagg cttttttatt tgcctctgcg gaagggacgt
     5701 tcttaggaat gttcatggcg gccgccaggc gggtggccag tttctggaag accggggcgc
     5761 atacggttcc gcctccggga tggcaatgct tggaagtggg gtcgtcaatg acgacaaggc
     5821 agacgaaggc cgggtcctcc accgggagca ttcctacaaa ggagacggtg tagcggtttt
     5881 caaaatagcc acctgtaggc ttcaccttgt gggcggttcc ggttttgcct cccacgttat
     5941 agccttcaac ccgcgctctt ttggcggtcc cgtccagatc cgtaacgtgg aacagggcgt
     6001 tccgaaggtc ccgggccgtc ttgacgctca tgacccggca tacctccacg gggggggaag
     6061 gctggaagtt cctgtcatcc acataaatgc cgtcaaccag ccgggggcgc atgcgcacgc
     6121 cgtcattggc tatggcggca taagccatgg cgacctgcag gggggacacc atcagggagt
     6181 agccgaagct gatgcgggaa aagttgacaa aattgctgcc gtcctggcac tggctgttgg
     6241 tttcccccgg caggtcgatg cccgttttgg aggaaaatcc gtacaggtca aagtattttt
     6301 tatacgtggg ccaacccgat ttcagtgcaa tgcggaaggc gcccggattg ctggattttt
     6361 tcagcacgcc cgctacggtc aggccgccat aggaacgggg ggcgtccgtg acggggcggg
     6421 aaccgggcac cggataaggg gtacagttga tcatggtgtc gaacgtggcc tttccgctgt
     6481 ccacggcgga tgtgacggaa actattttaa aggtggaacc gggttcgtac agggactgca
     6541 cggcgaaatg gtaggcgcct tcctgaaggc cctcgcgcgt atttaaatta tagtggggcc
     6601 ggctggccat cgccagaatg ctgccggtct tgggttccac cacaataatg cagccgcggg
     6661 gtttgtgggt ctggtccgtg taaaaggaga tggcggcgtc cagctcctcc tccacgatcg
     6721 tctggtattc catgtttacc gtcaggcgca cgttcagccc gtccacggca tccttgaagc
     6781 gggaatccgc ggagggaatg atgcgcccgc ggctgtcttt ccggtattcc ctgatgccgt
     6841 tgtggcccag cagctggtcg tccagctgct tttcaaggcc ggaaagggct acgggccggg
     6901 ggccgctgtc cttcgtctgg gcgatgtagc ccagaatatg caccatgcat tccggcacgg
     6961 aatagacgcg tttggaggag gtctcaaaac ggaacccctg gacgcgggcg ttctggatag
     7021 cctgccgcag cagttccgct ttttcctcgg acaggttttt ggcaatgacg atgcgcttct
     7081 tgggaatcgc cccgtccttt tccaccgtgt tgatgatgtc ctgcacgctc atgtccggca
     7141 ggaaaggggc aatcactttg gcggcgtatt ccaggtgcgc ctgcacgtat tctttcacca
     7201 tctccggctc ataaagctct tccagctttt ttctggccat atccacccct tccggtccgt
     7261 cttcaggctc ttccagcagg attttcgcca gattatgttc cttgctgccg tcctttttgc
     7321 tggcggcctg gcccagaatc ttgctgcgga agccggaaac gagcttttcc ttttccttat
     7381 ccgttgcggc tttctcccag aacggggaat ggacggcctt ggaataggcc agagcccagc
     7441 tgatggtctt ggggtcgttc agatgatagc cgtccgcaat cagctcggaa ctctgcatgt
     7501 tgctggtgag gatttcctca ttggcgtcca tgatgcggcc gcgctggggc aaaagaactt
     7561 cccgttcaat aatgccgccg ctggaaacgc cgctcgtttt ccggggggaa accagctgaa
     7621 gattgaccag cgacaacatc agccagatga ccgccgcgcc cgccaggccg cacaggacga
     7681 ggcttcttct ggcaaatctg gacttcgtga tcgtgatcat gaacggctca acgcagttaa
     7741 tggcctgccg cagcgatgcg ggaggcgttt tcggaagaaa tgtattctac ggctgaaggc
     7801 tcgataggct gcaggttgga cttgtcctgg gccagcctgt cgcggatggc ccagcggctg
     7861 gtcagggagg aagtcttgga acggtaatat tcctttgtaa gttcgcagga ggcgatctct
     7921 tcgttcattt gcttgatgtc gcgcgcgacc tggatctggt cgtttttcag cacggcataa
     7981 gtgatgcccg ctccgcaggt gagggcggcg aaggcaatga gccacatgat catgctcacg
     8041 ctgatctggt ggccggtgaa acgctgtttg aatcttttca tggcggtggc ggaagagaga
     8101 tggtcagatg attttttcca cgccgcgcaa cttggcgctg cgggaacggg ggttggttga
     8161 aagttcttct tctcccgcag tgacgggttt gcgggaaagc gggcggaagc agtaatccgg
     8221 gttggggcgg ggtgccggcc attccgggcg gtctatttcc ggacggctcc ggaggtcgaa
     8281 gaactgcttg accctgcggt cttccaggct gtggaaggtg atgacggcca tgcgcccccc
     8341 ctgcccgagc agacgcacgg aggaatccag cagagcgtcc agggcatcca gctcgccgtt
     8401 gaccgctatt ctcagggcct ggaacgtgcg ggtgccgggg tgttgccttc ctttgcgcgg
     8461 caggacggat tccacgatgc gggcaagctg tgcggtcgtg gtgatgggtg cctgggcacg
     8521 ggctttcacc atggcgcggg caatggcgcg ggaagcgcgt tcctcgccga attgccagag
     8581 gatatttgcg atttcttctt ccggggcctc attcaccagg ttccgggcgg agagagcggc
     8641 gcgggtatcc atccgcatgt ccagagggcc gtcttcccta aaggaaaagc cgcgggaggc
     8701 ggtgtcaagc tggtgggagg aaacgcccag gtccgccagc agcccgtcca cccgggaaac
     8761 cccctgctgg gaaagaatgc tttccacgtc ctggaagttg ccggcgagca ctttgaaacg
     8821 ggaaccgaag cgcgccagcc tgagcatggc ggcgcggcgg gcgtccgcat cgcggtcaat
     8881 gccccagaca gtggctccct gttccagcag aagttccgta tggccgccgc cgccaagcgt
     8941 gcagtccacg atgaatttgc cgggtcctgc cttcagcatg tccaccgtct catgcagcag
     9001 cacggtaatg tgccggaagc cgtttccgga gtctgccccg tccgcttccg catcaacggc
     9061 ggcagcggcg tcatccccca gttcggaaat cagggcgtct accctggccg cggcttcccg
     9121 ggctttcagg gcttccgtgt ggtacgcgtc ttcccagttc atggaggggg ccagcgcctt
     9181 ccaggagcag accagttcat ccactccttt ttcctccgtc agctccgcaa tcacatgcac
     9241 atggttgcgg cagacgctcc acgccagcac gcggcaggac gtttcttccg cgagcccgtt
     9301 tgccagggcc tgcacctcct ccgtgcggac gtgcgtaccc gcgcccgcgc gtaaagaaag
     9361 ctcaatatgg tcgtcaaaag cccggttcag gccggaagac aatgcgccca gcttgccata
     9421 ggcgcccttt tgcccggcca gggacatctc cgtcatgagg cgtgcctttt cctgctgcca
     9481 ggcaaccata tcctgcgccg gaagggcatc gtgcccccag aaaacgatgg aataagccgt
     9541 gagttccgca tgcagcccgg ttctggcgcc gacgcccagg ccggaagcca gccagcccgg
     9601 gcgcaatgca agcgttgcgc aggggcgtgc ctggggattg ggcgtaatgg aggagtctgg
     9661 gggcatgaag gaaagctaga gaatgccgta ggattggttg atgtcggaga tgctggcgtt
     9721 ttcacgccgg gaaacttctt cgtagagaga gggttcccac aattcgaaat atcctctccg
     9781 tgcggcgagc cggacggagg aactgagctg tgcgtgttcg cacatttgtt tgggtatcag
     9841 cagcttgcct tgggcgttga tgacagcctc cacacagttg gcgtataatt tgccgatgaa
     9901 aaggtcgatt tgcgcttccg tgtagccggg cgttgtttct atcttgtcaa tgagttgctg
     9961 gannnnnnnn nnctttgacg gtcggcaaat caagacgccg gccggataac aggagcaggg
    10021 cgcaaccttc ggagggcctc cattcggccg gaatggcaat ccggtttttg gggtccagct
    10081 tgtgcgtgta tgcaccaaaa aggttgtctg atacactgct catcgcatgc aagtacacta
    10141 cgtagtgact tcaatacact ttaatgcacc tgaaatgtca accccgtact ggaaaaaagt
    10201 attttttcat gtgcccatgt cataaaaaaa gcatggatac gttcttgctc ccttccgggg
    10261 gcggtattat attccgcgca tgatgcagtt ttgcgtgttg ggcagcggca gcgggggaaa
    10321 tgccaccatt gtcagggcgg gggaaaccgt cctgctggtg gatgccgggt tcagcgccgc
    10381 caggctgcgg gataagatga agtccgccgg agtggagccg gatgagctgg acgccattct
    10441 gcttacccac gaacacgcgg accacatgaa aggcgtgcac cagttcacca gaaagcatgc
    10501 cgtgcgtgtt tacgccacac gccatacggc catgtgcgtc caggaaaaag cgccggaggc
    10561 tccctgggcc tattttgaaa aggggcagtc tttcagaata ggggatatcg tcattactcc
    10621 cttccccact taccatgacg ccgtggaccc ggtgggattc aaatttgaaa cggagcggtc
    10681 cagcctgggt ttcatttccg atacggggca ggctcccggc tgcatagcgg aataccttgc
    10741 catggtggac agcctggtgg tggaatcaaa ctatgatccg gacatgctag cggccactcc
    10801 caggagacct tggccgctga agcagcgcat cgcctctgcg cacggtcatc tctccaatgg
    10861 gcaggcgtgc gacctgctga ggcggattgc tcatggagcc ctgaaaaacg tggtgctggc
    10921 gcacctgagc gcagaaagca attctccggc attggcagaa agcctgatgc gtgataccct
    10981 gcatgacatg gggctggctt ccacctccct gttctgcgcc agccaggact cctgcctgcc
    11041 gtggatacgg gtctgctgaa tatgcctctt ttcctgttcc tcctgccatg ttcctctcca
    11101 cctgtgaaat catgagcacg attattggag ccctggcggg ttccatcgct tcctcgcggg
    11161 tgaaaatgga tttgttcggc gtgattgtct gcggcaccct ggctgcgtta ggcggcggaa
    11221 ccgtgcgcga catcctgctg gacataccgg tgtactggac gctcccctcc ggggaaattt
    11281 ttgtcctggc ctccgtcgtc accagcctgg caacctttta tgtggcccag aaatatccgc
    11341 cacccatggg cactatccgc gtggcggatg ctatcgttct ggcccttttc ggcatgatcg
    11401 gcacggaaaa atcctatctc cacggctata cgcccactgt ttccgtgatg atgggcattt
    11461 gtaccggcgt ggcgggcggc ctgctgcgcg acgtgcttac cggcaacgtt ccctacgtgt
    11521 tccgtcccgg agaactatac gccaccgcgg ccctgctggg aggcgtggcg tacgcggtgc
    11581 tttactatgc cggaattgat tcttccacct gcttcgtgac gggcttcatg gtcacgcttt
    11641 ccgtacgcct ggcggccatc cgctggaact ggaacctgcc ttcctacatt ccgttattca
    11701 cctcggatac cgattcgcct cctgaagaac cggaaacttg acaggaggga atgttttcgt
    11761 gaagccttaa gagcgtcggc gcctgcggcg gcaaaaggca gttcccgccg cgagggtggt
    11821 gagaactgcc gtggaaggtt ccggaacatt ataaaagggc agcatcaggg cgtcgtatgc
    11881 gttgattcga gggccgttct cgctccccaa gtgcagggtt ccatccgttt catgataata
    11941 aacgtcccgt ttttccagac cttgtccgtt gtcggagtct gtgacgtaca gggatttggc
    12001 catgccggta gtgtcgtcaa cctcaattcc ccagcaggta atggcatgac ccggacctga
    12061 tgcaggagca atggaaagtc ccactacgcc gccattctgg aaaaatgaga cgaggtctgt
    12121 accgaactgg aggggaaagt cgctttgcgt gaagtatttg ctgctgatga aagggcagtc
    12181 gccttcccag gtggaagagg acaatcccag agatgtgata tatccctccc agtatcggcc
    12241 agtattctgc ggttcagtga tggtttcctt aaaatcgtat aaatagttgt tgggattgag
    12301 aggtttccct cccaggtacc accgcaggcc tatttctatg cctttcccgg tattggccca
    12361 attattgcaa aacacatcgt aaatttccgt tcttccgctt tcattctggc cgttgggaac
    12421 gaaagccgga atgccggagg aattattctg ccaccattgc agcatattgc tggctgtggc
    12481 cgcccagcat aaatcggaat ctccgttcca gtattttttg gtatttatga accccgaatc
    12541 ttcggaaaca ttatgggcaa aataaatggc ggcttgaaca ggaaccagag cgaaaaggaa
    12601 aaagagagaa agggtgcgca acataaaaca tgatatcgat agcgaattaa caagccggaa
    12661 tcaatgaaaa tatccgcaaa taaatttttg gatgattgcc atccttccac agaatggaga
    12721 ggaaaaaaga atctggagtc agaaaaacct gttgatgaaa gcttcttctt atttccccag
    12781 gcagaaggag gaaaaaatgg cgcccagcac gtcctccgta tccacggctc cggtgatttc
    12841 tcccaggtgc gtcagggcct ctctcagttc cagggctgtg aattccggac tttcctggcg
    12901 ggagatggat tccgaagcca gccggacatg ctcccggcag aggcccagtt catgctggtg
    12961 ccgggcattg atggctacca gggagctgcc cgtttcaccg ggcagggagg aggaaaaggc
    13021 ctggataatg gcttcctcca attcctttct tccttccccg gtggcgcagg aaaaccggat
    13081 gccgggcacg gccttccagt ccggatggac tcccaggtcg cacttgttca gaatgagcag
    13141 ccggggcgcg gtgagaacag gggcggggaa gtcttggatc cggggcgtgg aggcatccgc
    13201 cacttccaga accagatccg cggtttcaag agccctgttg gtgcgggtaa tgcctgcctg
    13261 ttcaatgacg tcggaagatt cgcggacacc ggccgtatca atcagccgca gggccagccc
    13321 ggccagctga atggattcct ccaccgtgtc ccgcgtggta ccggcaatat tgctgacaat
    13381 ggcccggtca tatcccagca gggtattcag caggctggat tttcccacgt tgggaggtcc
    13441 ggcgatcgcc gtgcggatgc cttcccgcag caggcgtccg ccttcggcag ttttgaggag
    13501 ggcggccagt ttttcctcca tgctccggag gcgcgccagc agatcggagg cggtgtccgg
    13561 ggaaatgtct tcatccggaa aatcaatata ggcctcaatg tgggccagca catgaaccag
    13621 gttgtctttc agttcgtcca cctgggcgcc gatgcctccg tccagctggg tctgcgcggc
    13681 tttcagggcc aggtcgctcc cggcggaaat cacatccatc acggcttccg cctgggtcag
    13741 gtccatgcgc ccgttcagaa aggccctttt tgtaaattct cccggttccg ctggggaggc
    13801 cccgcattgg tagaggcgtt tcagcaggcg gtccgtcacc agcatgccgc catggcagga
    13861 gatttccgcg gtatcttccc ccgtataact ggccggggcg gggaaccagg tgacaagcgc
    13921 ctggtcaatg gcggctcctt ccgcgtcccg gatgcagacc agcgtcgccc tgcgcggccg
    13981 gaggcgttcc gtgaaagccg caggggtgca ttgtttcagg atgtccatgc agccgggacc
    14041 gctcatgcgg atgacggcga tggctccctg tcctgcggcg gtggcctgtg cggctatggt
    14101 gcgttccggg tcagtcatgg ttctatggat tcaaaaaagc cgcccccgag caacttgcct
    14161 ccgtcgtaaa aggcgcagac ctgtcccacg gccagggccc ggaggggcgt gtcaaagccc
    14221 aggcgcacct ttccttcttc cagatattcg caggaggccc attccgcctt ggcccggtag
    14281 cggggctggg ccatgacgcg ggccggcagg ggagcgagcg tattggaaat gccgccgacc
    14341 acggcgcggg acgcgtacaa tccccgcgtg gacgcgtctt cgtatcccag aatcaggcgg
    14401 ttgcgctgga catcctttcc caccaccaca taggcgactc cctcccgcgg ggaggcgacg
    14461 ccgtgccctt tcctctggcc catggtgaac agatgcaggc cgttgtgcgt gccgagcgtc
    14521 cggccttccg tgtccacgat tttgccgggc ttgtccggca ggtagtggcg caggaaatcg
    14581 ctcattttta cctggccgat gaaacagatg ccctggctgt ctttcttgcg tgccgtgggg
    14641 aggccgtatt tttccgcaag aacgcgcact tccggtttca gcagatcccc aaggggaaag
    14701 acggcgcggg ctatctggtc cgggcgcatc agggacagga agtaggattg atccttgttc
    14761 gggtcccgcc cgcgcaggat gaaagcccct tgcggcgtgt ccatcctgcg ggcgtaatgg
    14821 ccggtggcga cgtaatcgaa cccctgttcc agggcataat ccagaaagac gccgaatttc
    14881 atttcccggt tgcacaatac gtccgggttg ggcgtatatc cggcgcggta gccttcaatc
    14941 agataattca cgatgcgggc gcggtattcg tccaccaggt caatgacccg gaattcaata
    15001 cctgttgttt tggcgacggc cagcgcgtcc tgaatgtcct gttcccacgg gcattcgccg
    15061 gggatgcctt cgtcattcac ccagtttttc atgtaggccc ccactacgtc atggccctgc
    15121 tccacgagca gggccgctgc cacggagctg tccactccgc ctgataagcc tacaagtatg
    15181 cgtgccacga tgctggaaaa gatatggggg tgataaggcg gaccgctttg agaaaggctt
    15241 tgccgcctgc ccctgtaaac caaagatgtt tccgtaagcc aagacaaaaa gcgtggaaat
    15301 ggggcatccg gtccctgatg cggaatatga ttcggtcgat ccgggaggct tttgattttt
    15361 aatcacattc ccnnnnnnnn nnaatcgaat ttggaatccg tagtcaatat aataaattag
    15421 aaaaaagtat tataattaat ttaccacctc tttaaacagg aagctggaat atggagaggt
    15481 aaggcccttc attttttttc aggaggtatt tccgtgatga ggaaagatga ggaggtatta
    15541 actaaaaaat tgttgttcaa tggcatattc attttttcag aatgcggtgg tctgatgatt
    15601 tcagtctgcc ggagaacgga ggccacctgg aaaaaagcta cggattccaa atccgtaccc
    15661 atataaaaat gtttcgttgt aatagggtaa atggatgttg tattgcatgg tggtctattt
    15721 tctcatttct gatttgttat tattccagtt atgtgtctcc ctattatgaa attcccgtaa
    15781 gttgtgatcc cattatttat catctcatgg gacgcggaat gatggaaggg ttgatgcctt
    15841 atcgggattt gtttgatcaa aaggggccct tcattttttt gatatatggc ttttcctacg
    15901 ccttgtgtgg ttcttactgg cttgtattcg ttcttgaggt attcgcagtg acggcctcca
    15961 tgatcttttg ttacaaggcg gcccttctct tcgtatctgg aaaaaaagcg tttatcgttt
    16021 cgctgctcat cctttattcc atgtgcagtt tccactatta tgcaggagga ggtcatccct
    16081 ccgaatttat tctgcctttc cagtttgccg cgatttatgg agtgtgccgc ctgtatcggg
    16141 atcccgatcg cttcgtaaga acgggcgtgg tgtttggcgc gggcatcggg atggcgcttc
    16201 tgttgaaatt caacttggcc gctttttggt tcgttccctt cctgtatgtc ctgtatcgtg
    16261 cgcggatgac cgggaaggca tggattttta caggaagcgt gggaggaacg ctcctgttga
    16321 tgctgacgcc ctgcctctgg tatttttaca gccgcggcgc gttgctggat ttatatgaag
    16381 gatacattgt ttttaatgcc ggttacggcg ccgatgcggt atcgttcaag gagatgtgcc
    16441 ggaattattc ccaatgggtg aaaagggata tcatgtatcc tagcatgttg atgtacatcg
    16501 tgggaggagc gggggtgatt ttaagccgga tgcccgccag ggaaaagatt tattatacag
    16561 ccgcttttct gattacctgc atgggcgtcc agggaaatgg aaagacgcat ttcaaccatt
    16621 acgcccagac acttattccc ttcgctgctg tgggatacct ggtggtggcc aggatgatcc
    16681 atgtggaaga ggttgtttcc cgaaagtggc gccgatggct ttttcctctg gttgttgcgg
    16741 gagtgatggc ttgcaccttc ctgaattgcg tggatttttc atgcagaagc cgcgcccgcg
    16801 aatggatgta cgcttttgct cttccagata ccagtgatga accgggggag aatgcttgtg
    16861 cagtccttgg gcggcatgcc gtatggtttt actgtgtcaa tggtttggtg ccgcccatac
    16921 gcacgtttac gatttgcgct tccgacaaac agggtgcaga acgggaaagg atgcgtcagt
    16981 atcaggaaat ccgccgcgga aagatagact atgtggtgat agcgggagaa tacttcaagc
    17041 cggacaacac ggaatctccc tccctgaggg ctatgcggga tttgctaagt acggaatatt
    17101 gccgggaacg atcctttttc agagaagtga cgaacttgga tatttaccgc cgtgtgaaga
    17161 gggattaatg ccccagttct tccatgatgc gttctgcaag agcccgggga gagtcggaag
    17221 gggaagccgg aagtttccga agccattgtt cgcgtttcag ccaggtgcgc tgccgtttgg
    17281 cgtattgccg ggtggcgagg gctagctggc tctggcagtt ttcacgggag atttccccgc
    17341 gcagcatgct tcgtatcaac gccaggccca gggtgcgttc cgctgtggcg gagcatgggc
    17401 cgagggaagc cgtttcctcc acggccccct cctgcatcat gcgtgcggca cggtgggcta
    17461 ttcttccgtc cagttcagga acatccctgc tgatgaccca gccgggcccg cgcggcgggg
    17521 tcagccagtt cctcttccag aaggaaagag gtttccctcc ggcaaggacg atttccagat
    17581 tgcgttccac ataacgccgg ttggaggcat ccgtcgaggc cgctccttcc ggatccaggg
    17641 aagagagtct ggcgtatagc gcttccgtgg aacagttcgc gaaagcggcg cggagagcag
    17701 cgtcccccgg cggcgcttcc gacaggccgt gggagaggaa tttgaagtac aggccggaac
    17761 cgcccgtgac gatggcgatt tttccgcgtg cgtgaatttc ttccatgcag cgcatggcgc
    17821 ggtggtagtg ttccgtggca ttccagttcc tttggaccgg aatgatggaa accatgtggt
    17881 gggggatccg ttcccggtct gccggggaag gcgccgccgt caggatgggt agctcccggt
    17941 acacctggta agcgtcggaa ctgacgattt ctgcgtccag catctccgcc agttccacgg
    18001 ctaccgcgga tttgcccgat ccggtgggac ctgccagaaa cagggtcatg ggaagtgttg
    18061 cctgatgggg tggagaagat tcaatcatca cgtggagtaa tggcggataa agtttgttgt
    18121 tcctgtgaag ccctaaaagc tgctcctgct taaaagctgt tggaggaagc ggaaaagcgg
    18181 aggcgaaatg gcttcatatc ccagggcgtt gagatggagg ccgtcccaag tataaaggct
    18241 ggcgtctttt cccggaagga agaaatgaag ggggaggaca tggacatttt ttccaaaatc
    18301 gtgttgcagc agacaattcc tgaaggtttc catgcgcgac cctttttgtg cgatgagcgg
    18361 gggaatgaca atgatggcgg tcgtttccgg gctgtaaatc tccctgattt ttttgacgat
    18421 agccacgttt ccctgaaaag ccgtccatcc caggcttttt tgtactccca gattgttgac
    18481 tcccgaatac aggacgatga ttctgggcgc cagcgggctt gtcaggccgt cattgaggcg
    18541 ccagagcagg ttgcccgtgg tctcgctcct ggttcccatg ttgaggcatc ccagagggga
    18601 aaaccaggtt tcccaggaat gttttccttc tttcagccag aattccatgg tggaggaacc
    18661 caggaacaac acgggggcat ggggattcag ccgggcattg cgtttgtcct gccggtgttt
    18721 ttccagcagg gcgccctgtt ccgggtaata gggaagggga tggacagcca aatggtagcg
    18781 gtaggcgtgt ttggcgcacc agacggcgcc taatgcggcc agaacggtta aaagaagaaa
    18841 gaggatacag gccttaattt tttttgggaa catgggtgat gcggtcgttg gcgacgtagg
    18901 gcaggcaggc cgggaagaat tttctcagga aaagatagaa tgcgacgatc aggaaaatga
    18961 tggccggtat taatacaaag ggcgcgaaaa aggattgttt ccagatgtgg gaggggatca
    19021 gcatgatcaa aggataatga agaacgaata tcaggaaaca ggcgggagca tagttggaca
    19081 gctttctggc aacgggaatt ttcttttcca gccagattcc aagggccatg agaagaagcg
    19141 ccccgaagac tgggcagagg gaattcaaat ggacagccgg ccaggagata tcccatactc
    19201 cataggatgc cagtattttg tgcatgttcc attccgggtt attctggcaa acgctcaagt
    19261 aattggtgat gctgagcaga aacccggttc ctacggcgag caggacgagg ggagtaaagg
    19321 aatttttgta aacgtgtttt acttcagcca gtttgatgga agacaggaaa aggcccagac
    19381 tgaacaggga gacggtggat ggagcaaggg taatcttgtt ggccggcacg gggtattgga
    19441 gccagtccgt gctgaagcag atcaggatca ggacaaaaac cggatatttc aatttcagca
    19501 ggatggggga aatgagggaa cagagaatga tatccctcaa aaaccatgtg ggaggggaaa
    19561 agggttctcc cgtcaatcca ttgatggaaa gaggaataaa atcgggagtg aaaagggagc
    19621 ggaagcctgc aaggttgcat aagtattccg gcaatcccat attcatgatt cccggatgga
    19681 ggaaagagat gactatattc cagaacagaa agggaatgag aagcgtataa ccccgtttga
    19741 acgctttgtt ccaggaatta ttccggccca tgaaatagcc tgccagaatc aggaaaaatg
    19801 ccacgcggcc gttgaaaact ggaagctgga acaggctggc gaaagagaag gccggcgcgt
    19861 ggacgcatac aatcaggcat gccgcgagga atcgggatat gtcaatccaa tgttcccggg
    19921 atgagggtaa taaaattgta ggattttgcg gtgtcatgaa agagtctgcc tttggagccc
    19981 cctgaggcag gcaggggaaa tgctcaaggg cagatggctt tttgtgtttc ccggggatgt
    20041 ttgagacgtt cggggagcgg tttatttact gcgggatttc cgatggccag gcagccggga
    20101 ggcacatccc gggtgacgac ggctcctgcg gcgactacgg aaccggagcc gatggtgact
    20161 cctttcaaga tggtggccct ggcgccgatg aagcatcctt ccccgatgga aatgggttgt
    20221 ccctgttcgg gcttccccag ggttgatccc cacctggcgc cgctccgggg atagtgcatg
    20281 tcgttgtcca gaatcagcgc gtctgctcct atcagcgtgt ggcggccgat gcggatgccg
    20341 ttgacgcaga ccagggtggc tccgctgatg ccgcagccgt cctccagagt gatgcttgcc
    20401 tgattggaaa gggcggccag gcaggtgtga tgggaaagta ccggattcat ccgggtcagg
    20461 gagtggatgg tgactccgtt gccgatggtg atggacgctc ccgggaattt tttaatgagg
    20521 ggaaatccgg aaagaatgat gcctgttccg gtgcgcaccc ccctgagcct gagcagcagg
    20581 cggcctatcc cggaaagcag atggataagg aataatttca tcagcgtgga tacagcatgg
    20641 tatgataacg tgacaggcac ccggttactg tctgctttac gtccagcagg cggcggcatt
    20701 cctgatgccg ctccattccc atggacatgg ctttatccag gctgacggaa agtttctgcg
    20761 cggcttccct gacggcggcg ctgttgcgga cagggacaat atagccggat acgccgtcct
    20821 gaacatattg agtctgtccg ccttcttccg tggtgattac cggaaggccc atgactcttg
    20881 cctccttcac gatgttgggg cagcaatccc ccagggtggg atgcaccagg aaccacgccg
    20941 tggagaggtg ctgctgaagt tctgcgcggg aaatgctgcc cagccagtgg atattgggag
    21001 tggacagggc ttccagcctt tccctgaggg ctccgttgcc cgcaatgaac agctggacat
    21061 gggaaaggga cggatgcgtg aaagcctcca ctaggtagga aatgcccttc aactcgtaaa
    21121 tggttccggc aaagaggcag gaaggttccg gggaaggttt tctggcaaga tggtaaaagg
    21181 aaggttccac gccgtattcc atgcaggata gacgggcatg gggggcaatt tccgcaacgc
    21241 ggccgcacgc ccagggggat tcgcacgtga taagatcata atgtttgacg gccatccgtt
    21301 cccagagggc ctgcatatgg aggaggaagg cctgcggagc gcgctggcag taggcggtga
    21361 gggctccctg gtaggaaagg agcttctttc cccggaaggc aatccccgcc agagcgtaag
    21421 cctgctccac gccccatacg tgcaggaggt ccggctggat atcgttcagg gtcttgcgta
    21481 ttctccggac ggtcaggaaa tgggccgtca ggatattcct gcccatgctg cccaggggaa
    21541 gaatgtggat ggtctggttg cgcatccgga tggtttccgg ggcagaaacg gctttgctga
    21601 gggtgatcca gtggatgtca aaatcttcct gagattccag agcatgaaaa agggaaaaaa
    21661 tccagggaat cgtacgccgg tccttattac ggaaagacgt cagcagccgg cccagaggga
    21721 ggtccgctgc tatggctaat acgggacgga ggcgtttgat cggcatggat tggaaagagg
    21781 aggcaaggtt agcaggtggg taaatgctcc cggacgatgg aagatgcgaa cggaggcttt
    21841 ccttccgggc ggatggagga aaaaagcagt tgctcccatt cctggtttac tgcgtcgggg
    21901 gcgaaacagg cttcccattg ccggcggcct ttactcccca gtagtttgcg tgttttttcg
    21961 tccgtcaacg attccacggc tttttccagc acggaggggg aagtgttttt caggacgatt
    22021 cccccctcca gggtggaaag agctcctgcg tccggagcga accagggaac gccgcatgac
    22081 atcgcctcca gaatggagag gggctgctgt tcctgataag agtaaaggaa aacggcatcg
    22141 ctttccataa tggcggcttc tacttccaaa cgggggagcc tgtagagaat cctgaatctg
    22201 gggtcatccc cgatggcttg ttccatttgt tccgcgtagg cattgtgctg gggaatcaga
    22261 aaaagaaaaa tgatatgccg cagacgggag cgtttcacat ggcggatgaa gagaagctgc
    22321 cgttttctca tgctggcgcc ggcagggcag gtaaaaacgg gggcggagga aagtccgtat
    22381 ttttcccgga aaaaaggagc ggttcttttc agagccggga atgtgttggg aatgcaggag
    22441 taatgggcaa gtttgcttct ggatgcgcag taataatcaa agctcttgaa cagtgtcccg
    22501 taaggatcga gaaaaacgag acggtttaac ggtgatttcc ggcatacgtt gaggatatcc
    22561 ctgcgtactt gaaaaaagga gcagagccat tttttcaggc cgaagaaagg agggtaattg
    22621 aagcggaaaa aagagcggaa attggtagct gcaccatggg agcggaggaa aaacggaatg
    22681 cctgaatccc gtgccgcgtc cagcaattct tcccggcacc agtccgccca ggagtggtag
    22741 ataataacat ccggccggaa atctttcaga aacccgtcca ggtattccct taaacgggca
    22801 ctgtccttcg tcagggtgtc cggcagggtt gtgagagggc tgtattggtg ggagcggaag
    22861 gactcatccg gtgaatccag cgggcgggtg aggacgtgga tatcccagtc cagggagctc
    22921 aggctgcagg ccatttcata ggcagccatg cctacgcctc cttcctccgg aggaaaaaca
    22981 tatgtaacga tgagaaccct gcccttgctc acgggcggta atgtagatga aaagaaaaaa
    23041 taaaacaagg acactttatg gaactgttcc ggtccatgga acctgtaaaa atgaggaatt
    23101 tgttttttat ccggtgagcg ataaggaggc gtcatgggaa caggcgattc tgccaggttt
    23161 ctatcccctg tgaaggcctg ttggctgagg cttcataaaa agatattgat gcggcaggat
    23221 gttgtgctag attggcaccg ctttccgtac ccgtatagcc gcaatatgcc gttggtatat
    23281 tgccggttga ataaataatc cggttattaa taaaacagat atttttaact tctcccggga
    23341 aaggataagg ttgatggaat aaataacatg gaagaatccc gcgtaaagaa aagcctgttg
    23401 aacgccaggg tcaatctcat tttttatgtt ttgattcttc tcatttcctt tttttcaagg
    23461 aagatattct tgaattgcct gggtgccgat tttgtcggtt tgacgggaac attaatgagt
    23521 ctgctgaatt tcctcaattt ggcggaactc ggtataagca cggctatcgg atatgtgctt
    23581 tacaagccta tctttgagca tgatgagacc aggattaatg aaattatctc cgtgctggga
    23641 tacatgtacc ggtggatagg gcggataatc atcgcggctg gagtagcgct ttcctgtttc
    23701 ctgccggtta ttttcccttc cacaggtttt tcctatggaa tcatttattt tgcttattat
    23761 tcctttctgg cctcttccct gatcgggtat tttgccaact acaggcagac ccttttgggg
    23821 gcggaccaga ggaattacgt agtggcgttt tatttccaga cggcatccat catcaaaatc
    23881 atatgccata tgctgtctgc atggtatacg ggaagttatt atttatggat agggatagag
    23941 ctgttcttcg gcattattta ttccttcatc ctgaattgga agatcaatca ggtctatccc
    24001 tggctgaaga gcaatgtggc cctggggaag gagctgttca aaaaatatcc tgaagtcact
    24061 aaatacacca agcagttgtt tatgcatcgg ctgggaagct ttttccagtt ccaggtgacg
    24121 ccgtttctgg tatatgcctt tgtttccctc cagatggcgg cctacttcgg caactattcc
    24181 accgttgtgg agaaactgca ccttctggtc aataatctgc tcggcagtac ggaagccagt
    24241 gtgggcaact tgattgcgga gggaaatgcg ggaaaaatcc gcaaggtgtt cagtgaattg
    24301 ttttccctgc gcatgctgat tgccgggacg gtatgtttcc ccctttacca gcttttggag
    24361 ccattcatca cgctttggct gggagcgggc tatatccttc cccgggaaat catgctgctg
    24421 ctcgttatca ggctgttcat caccattacg cggggggttg tggatcagtt tttatacggt
    24481 tacggtttgt tctgggatgt atgggctcct cttgcggaaa gcgtgatcaa catttccgtg
    24541 gcgatcatcg gaggttactt atggggattg cccggcgttt tgttgggggg catctccagc
    24601 ctgatactta ttgtgggcat atggaagcct ttcatgttgt ataactgggg attcaggaaa
    24661 aatgtgatga actactggtt ccaattcggt taccagctgg cgctgatttt gatttcggcc
    24721 gtgttcatgg gctggatctg gaaatttgtt cccctgcagc cttccgcgtc tttcttttcc
    24781 tggtttttat gcgccttcct catggccgct tcttatggag gtgtgacggt tcttttaatg
    24841 tattgcggta cgcagggcat gcgcggaatg gtgcgccgtg cgctggagat ggcgggaacc
    24901 cggttgccgt ggcggaaaaa aactgaatag ggacgcagag gtgttgctgg gtatcgggaa
    24961 tggggacgct gcgtcattca tgctcaatct tcggtaattg tttcggcttc cgggtcaaca
    25021 aaatcctcca ccgcctcgga gacaatgata ttgctctcca tggaggaaaa accgtaatac
    25081 gccggattga cgttattgca atgcaagtgc atcgtcgcag gaaggaaagc tccggataac
    25141 gcccagctgt tggtattctc catatcaaga tagtacccgg aaaatggata aatactctca
    25201 aaacaatgga ctcccttcac cgtggcgatt ttgacgcagc cgcgggagga attcccgcca
    25261 tgcagcagcc acagcgcacc cctgtcttcg gtatcatatc ctccgtcctg gtattgttcg
    25321 taaaccaccg cgtacacgca gacgggataa ccgttattgg aagacgtctc ttgcgcgagg
    25381 gcctgcgtcg tcttcatctt ccatttctgt tcggcattgg aataatagat ctcccggaca
    25441 cgaatgcggt atccccccgc gtccgcgtcc cggataggat caaacgtaat atcgataatc
    25501 tccccaatct tgtagcctcc agccgcctga tccggcacca gcgtaaacgc gtccctgtcc
    25561 cgccccatgc gcgcaaccgt tgtcatctgg ccgaacctgg ccgtgaattt cgtgctgaca
    25621 gacggcagcc gcagcggggc cacccacccc cggatgctgg aataattgtg catgggcctc
    25681 gcgttggtca cgaggccgca tttcacggta aaggcggaat tgggagggac attgaaataa
    25741 agggaattgg gttccttgtc tgttttgaaa accgtcccat tggaagacga gcaatccgcc
    25801 aggagcggat aacacctgac ggaaaaagcg tcagttacgg cagacagccc ggcggcgtac
    25861 aggcggttga ccgctgacgt atccgttgcc gcccccacgg ccagcgggat gttgatgcct
    25921 ccgttggcgt tgacggcccc tgcaaaagtc ccggactcag ccgtcaggtt ttttaattcg
    25981 gcattttctc cttgcctgat gccgttctgg cctggcaacc ctggaacggc tgttttgaaa
    26041 acctgggtga tcagggcttc ttcatcggat acttcgacgg cgtccgtcgg agccgcaaaa
    26101 ccgtattgcc cttttttctc tgcttccaga ataagcatgg acgtctctcc ggaattgaag
    26161 atggcgctta cagtacaagc ttttgtacag gtcatgacat aggaacnnnn nnnnnntccc
    26221 ttaatagtat caactttcat aaataaatta tttttttaac tattgataaa actccggaaa
    26281 gatcatcagg cataaaacgt tgagacgttt ctgaagaagt atgcgggcgg ttctttccgg
    26341 attcgggaaa attccggaat gaagctgagc cggcttgtta aaaaaatagc cggctccggt
    26401 atttccccat aaaaggcggt tttgtaaatg gccgggtgtc agaaaaataa ggccttccct
    26461 ccgtatttct actgatgcct gccttgtaag agccgtttac cttttcccag gaggcatctc
    26521 attttggaga tgaacatccg gccgggatcg ttttgcttcc ggaaatccct gatgtcctct
    26581 ttccacggag atttcttcca atacttgtag aacaatccgt aatcctggcg aatgctgttg
    26641 ggatgccagg gtttgggatg gccccagtag tggataatgc ccggccgcct ggcggcttcc
    26701 agcaggtctg ccggctttag gggattattt ctgaagcttt ttacgccttc ccagcgaatg
    26761 gaccagatag ccccgtcgca aatgttccac atggggtgaa gcaacaagat acgctgctga
    26821 tagcatacat tcaaaatatc ctgatcctgc caggaaaggc ggtgaatatt gtttttcgcc
    26881 caggataaaa cctgggattc aaaacattct tcacgtattc ttccgatatg gtaaagaatg
    26941 acgccggcat tgatgtaaat ggagtcccgg tccagattca tttctctctt ccaacgagaa
    27001 aaagactcct cggataaatc ttccgtggtg aaaacgccgg caatgaggca atctccgaat
    27061 tcggattcat acatggggct tatgttcctg ttgaaaatgg tatcaatatc cacgtagagg
    27121 gcgcgttcta ccgtggcggg aagttcccct gcgaggccaa gacggtaata cgtcgccgcc
    27181 gaccatttgg aaccgacggg gaaatccgta tatttatcct tcatgtcaat gaagtgcaaa
    27241 tggagattgt agccctgttg cagggaatgg aaccgggccg tattatcccc gcttaacccg
    27301 ccagtcatga tgtaaatatc ggcatgaacg ccgggcggca aattctccat gagggacaca
    27361 atgctcacgc ccgttcccag cgcgccgtta tcgtctgtag cgtatacgat attcatgagc
    27421 gtgaacggac tggatcttgg cgtttgctct ccattccgtt attggtttgc agccggaaaa
    27481 acagggagcg gcccatcctt tccaggcggt ccagccattt gcaggagaac atggttctgt
    27541 ccatgagcgc ggaaaatgac tgttccatgc ctgcccttgc ggaacacgct ttcatatagg
    27601 agtccaaaaa cggccggttc aggcatgggt gcatgatttt cctgatcctg cccgtcaggg
    27661 aaatccgctc ctctgcgttg agcgataaca gtcttttggg atgtaattcc ctgatgacca
    27721 gataatggac ggcaaattgc catttgggat ttttcatggt ttcaagctca ttcagcagag
    27781 gcgcaatgag tgagtggagg gagacatgct ggtaatgctc atcatgcatg gcggaagaag
    27841 ctctctgcct gtaaatggag gtgacggagc ctcccagccc gatcaggcct gtcccccgca
    27901 tgcaccgtgt ccagtacagg ccgtcttccc catagggcat cgcttcttcc caggggggaa
    27961 gccgggacgc aagagacgaa ttcaggatta cgctgctggg atgcatgaaa agcactcccc
    28021 gtatccccag cctccggata gattcgggat aagatggagg gcgcggcggg atggcctcca
    28081 gttcatccct catgtagcgg ttggcatagg ccaatatctc cggatgacgg ttgaaaaaat
    28141 cggctgccag ctccagatgc tcgggatacc agtaatcgtc tccgtccagg aaagcgatgt
    28201 aagcccccac ggccttcttc atccctgcgt ttcgcgctcg cgatacgccg ccgttaactt
    28261 gggaaaccag gcgaattctg gggtcatgaa aagattggat gatggaaaca gtctcatcgg
    28321 aagaaccgtc atccacgatg atgatctcaa aatcctgaaa tgtttgaacc agtactgact
    28381 gaagggtggc agcaatataa ttctccagat tataggccgg gataataatg ctgaagaacg
    28441 gtttctgcat acaaggggaa tgatgaactg cttcgtgagt ggaacgggaa tcaggatatg
    28501 gaggacatga ttctgatggg gctgtaacca ttgcaaccgg tgaatggcag gggacgaatc
    28561 catcgttcct cccatgattc atcagtgcgc gaatgaacct gtcatagcga attccaaat
//
LOCUS       DAWEMX010000013        27299 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_723_length_27299_cov_12.236492, whole genome shotgun sequence.
ACCESSION   DAWEMX010000013 DAWEMX010000000
VERSION     DAWEMX010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 27299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27299
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_27299_cov_12.236492"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1794..1803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6735 a         7872 c         6781 g         5901 t
ORIGIN      
        1 atccagcaga atcacttcgc tgatcattac ccccaggaaa agcagcatca cttcatgata
       61 actgaacatg tccgggtgaa aaataccgtt gcgggctact tccatcatgc ctccggagaa
      121 acaggccccc accagcacgc cgatggaagc tacggtcagg atcacccgca cgggtgcagc
      181 cttggaaccg atggcggaat tgaggaagtt gacagcgtca ttcgctacgc cgttgatcaa
      241 gtcaaatgcc gccagaagca gcagggctgt gatgataatc cagtaaattg agtccataac
      301 tgtctcttgt gaatggtggg cggagggcat ccgcgagctg caccaccgcc tctttacacg
      361 gaaacggtcg attttgctac tgttttgtta cagaaaaatc ctttctcctt atcaccggga
      421 acggggaagg aaatccagtt ccccggggtc gggccaacgg tgccggggat aacgccccgc
      481 caggtccttt ttcatttgcc cgtatccgtt ccgccaaaaa ctttccagag atcccgttac
      541 ctgccacggc ctctggctgg gggccagcac ctccacaatc acgggtacgg ccccgttggc
      601 aatgcgtggg gaaaccgcca cgccgaacag gcgctgcacc gtcagactga tcaccgggga
      661 ggaatcctcc ccgtacctga ccttggcgtg ctggccgttg ggcaacgtga ggcttaccgg
      721 ggcataatcg tccagcgcct tcgcctgcca gccggaaagc caatccctca gcacgggaat
      781 aatttccctt tccttgatgt ccttataacc cacggcccct tcacagacca aagaaatggc
      841 gaccagcttg tcctcctccg aaaaccgggg cagctccagt tccggcatcc aaacgctcaa
      901 accattaaga cggcgtatcc actgttccac ggcatcattc catttcacca gcttcagggt
      961 tccgtccact actttttcag ccaaaaccgg ggcggccagc tccggagggg catccccgcg
     1021 gtcacgatcc tccagcacca gatctccata catcagcttc cggtgcagac gcacgcgccg
     1081 caaagcggca tcgaagacgg cttcctcccc gcaggaaaaa cgctccggcc aaatggaacg
     1141 caaatcttcc ggcgcaataa gcgtacagcg tcccacacgg gtttccaccg cctttccgga
     1201 caattccgcc acctctgcgg ctacgaaatg ctctccttga aacaccacgc ttccttccgc
     1261 cagcctgccg cccaccccgc cggccatgcg gcatgtattg gctgccacgc catttcgcac
     1321 acccacatgg tccgcaaaac tctcaatcat tgcacggact acggcagggc gcgccgccgt
     1381 aaaatccggt cccggcgttt cccgggcttt tccccggctg ccgacggaca gaagctgccg
     1441 gtatgccatc catgcctccc gcgccccgcg ggaagagatg ccccagcggg tacacgccgc
     1501 cgccccaaaa ccggcatcca cagccttctc caccgcgcgc cactccgcct ggaaatccgt
     1561 gtaatccgca gaatcgcgca aatgatcgtt cagccccccc tttaccgcaa caccttctcc
     1621 ctgcatcagg gctgcaacgg cggccagctc cacggcacac tgttcatcct ggccggccac
     1681 catcagacgg gccagccggg gcggcagggg aaaagcggtc atcctttttc ccgtatccgt
     1741 caggcttcca tcctcttccg tcgcacccag ggcagccaac aaattcacgg cccnnnnnnn
     1801 nnnagggggg caggaacatc cagccaacgg aaattttccg gtacggtgac gccccaggca
     1861 gccaaattca aaacggctcc ggccagatcc acccggaaac attcgggcgg gtcaaaatcc
     1921 tccttccggg cctgttccgc ctcactccac agccggaaac agcgccccgg agccacgcgc
     1981 ccggcacggc ccgcgcgctg agcggcggac gccttggaaa tcttcaccag atgcagcgta
     2041 tccatccccc ggtacggatc ccatccggac cggcggacca gcccggaatc aaccaccgta
     2101 cgcactccct caatcgtcag ggaagtttcc gccacattag tggaaacaat gacacggggg
     2161 atatcccccc gctccacggc gcaattctgc tgctccggcg ccagagaccc gtaaagggga
     2221 aaaacgtccc ggccgctcat ccacggcttt cctgccagca actccaccgt tttccgtatc
     2281 tcatacacgc ccggcataaa taccagcaca tccccgcagt catcctccct caccgcctcc
     2341 cgcacaacat ccgccgcctg ctcccatatg gggggaggag ccaccctgcc gcgtccgtca
     2401 ctgaccaggc ggggagcccg gtaaacgact tccacgggat actgcctgcc ttgcgcttcc
     2461 agaacccggc aggaacctcc catatattcc cgcagccctg atatctccag tgtggcggac
     2521 atcacgacca cggccagatc ccggcgcggc ccttcctgca gatccagcag acgccccagg
     2581 gataaatccc cggaaaggct gcgttcatgg aactcatcaa acaccacggc gctcacccct
     2641 tccagggagg gccattccgt cagccagcgc tccaacatgc cgtccgtcac gtacgcgata
     2701 cgggtgcggg aggaaatgta ccgttcaaaa cgcaccgcat accccacttc ttttcccagc
     2761 tccaccccgc gcagccgggc cacatgccgc gccagcaggc gagcggccat ccgccggggc
     2821 tgtacaacga caatcaggcc acggtctccc agaccggcgt catccatcat cggaggaacg
     2881 ccggtggact tgccggagcc ggtaggagct ttcaacaaaa tgcgcggaga aggaacccgc
     2941 agagcctcca tcaattctcc gcggatttcc tcaatgggaa gagaagaaaa aaccataccg
     3001 ggttacaggg aatccgttcg tgccggggac attcagccga tgcaggccag cagaaaccag
     3061 aaaaacggca cggtaaagga agggctgtca atcaaatcaa aaattcctcc tatccccggc
     3121 agcagggaac cggaatcctt gatggccagg ctgcgtttaa ccagggaccc ggccagatcc
     3181 ccgtaaacgg aaagcaggaa aatgagggat gtgcatccca tcagtaccag tacctgggtt
     3241 atggacaaga cggaaattct gaacatcggg aaaaccagcc ctatggccgc cgcattcgtc
     3301 agcacccagg aaccgaggag gccctcccac gttttcttgg ggctgatgtg gggaatgagc
     3361 ctgcgggaaa aaaggcggcc gcccagcagg aacccgctca cgtaggcaaa aatgtccgcc
     3421 atcttcgtta ccagaacgac ccacagcaca cacatgacaa tggccggata aacatacccc
     3481 gccctcatat acgccagatg cagggccaga atggcaaaag agaacagcca gacgggataa
     3541 ataaaggaaa gcagcgtagt acccacggaa cggagagccc tgcttccatc caccgggtgc
     3601 ttcatctcac ggatgaaggc aaccactacc aggaggaccg gagcggcaag cgccaccaga
     3661 agaggaaggg ggatgaggaa agaaacctgt tccgcaaggg cgctttcccg gaatttagtg
     3721 atgcagcatg accaggacag caatacgggg tacgccactc cgaaaagaaa gctcaagttc
     3781 ggctgggagg cttttccgga acgcagcagc atgtgccgcc attcccaggc ggccaaaata
     3841 cagaaaatgc agaccagacc gtaataccca agttcgcagt cccaccacaa agccccggcc
     3901 agcaacccaa gcaaaaccac ggtgctgaat gaacgctgca gcagaacacc catcttgctt
     3961 ccggaaaaac tcttgtcccg attcggtatg ctcatcatca cgcgaagtgt acagaaaacc
     4021 gttacaacct caatcctgaa atgcttcctt tcctgcggcg tctcttcgga aaacgtccgt
     4081 tctccccatc ctcaccctcc ccgctgcaaa caggaaagca gtcatcccgc cacttttccc
     4141 tctccgggaa ggatgcagca acggaaaacc aaagaaagca cgcaacaaca aaaagcgaaa
     4201 aaaatgcaaa aaaacatccg taaaataaaa tccatttcca tgcagctcca atatgacaaa
     4261 tgccggactt ccgcacaaaa tttcaaactt tatggtggat ttatccaccc atgaattctc
     4321 ttctttcctc cccctgccgc cgccagatat ttccggatga accggcttcc atttactctt
     4381 gtccccttcc ggaaacaagg agagcaaaat agcgtcatgt ggacgtttta taagatagac
     4441 aatcacctgc aaatcagtaa cacttacatc ccggcgccag ctttttcccc aaatggaaaa
     4501 aaacgcaccc ttttccgtta tgaacatgca ctcattccgt tggattagac tcaccgcatt
     4561 ctcggccctg gccgcagccg ccattacttc ctgcgcctct gcggctacgg acttcaacca
     4621 ggtgggcaag caaatgtccc tgctgctcca gaatttccat ttctcccgca aagaattcag
     4681 cgatgaacta tccactaaat tcctggaaac ctacctgcgc aaggtagacc ccaacaaaat
     4741 attcttcacc cagcaggacg tagacgccct caaaagaaaa tacggtaagg agctggacga
     4801 ctaccttatg tccggccaga tgatggatgc ggcccaggcc atgcacgccc tttaccgcca
     4861 gcgcgccatg cagcgcatct cctatgcgcg ggatttgctg aaaaagggag gcttcacctt
     4921 tgacaaagac aagtctatcg aacgttcccg ccgcaaaaca gccgcgtggc ccaaggatga
     4981 ggcggaaatg cagcaggtct ggaaagacat ggtggaggaa cagctcctgt ccgaaatcct
     5041 gcgccgtgaa accgtagcgc gcctggccaa ggaacagaac aagcccgatc ccctggccaa
     5101 tgaaaaaccc gcggaggaaa aactgcttat gcgttatgaa cgcattcaac gcaatattca
     5161 ggaaacggat ctggaagacg tagcggaaac actgctcagc gccgtagcct tgacgtatga
     5221 cccgcatacg gattacatgg gtgcgcgcca ggtggaccgt ttcaaaatct ccatgggtac
     5281 ggaactcacc ggcatcggcg ccctgttggg cagtgaagac gacggttcca ccaaaattac
     5341 cggtatcgtt gtgggaggac cggctgacaa atccggagaa ttgaagctga acgaccgcat
     5401 cgttgccatt gactccgaca actccggaga aatggtggat atcctgttca tgaagctgga
     5461 caaagtggtg gatatgatcc gcggagccga aaatacccag atgcgcctga aagtagagcc
     5521 ggcagacgcc cccggacagg ccaaaatcat tacgctgacc cgctccaagg tacctctgaa
     5581 ggatgaactt gccaaaggtg aaatcattga acttaccgga gctccggaag gcaggaaccg
     5641 cattggcgtg ctgagccttc cctcattcta cgcagacatg gaaggcggag accgccgctg
     5701 tgccaaggat gtcaaaaaaa ttctggaacg gatgaacaag gaaaatgtgg atggcctggt
     5761 aattgacctg cgcagcaacg gcggcggttc cctggaggaa gtgcgcctga tgacgggctt
     5821 ctttaccgga aacggccccg tggtgcaaat caaggacacc cgcggcaacg tggatatcaa
     5881 atccgcccac aaccgccaga aactcttcaa tggccccatt gtggtgctca tcaataaact
     5941 cagcgcatcc gcctctgaaa ttctggctgc ggcccttcag gattacggcc gcgccgtgat
     6001 tgtgggggat gaatccacct tcgggaaggg gtctgtgcag cagcctgtgg acatcggcca
     6061 atacctgcct ttcttcgcgg ccagagaccg tgcgggcctg ctgaaagtca ctacccagaa
     6121 attttaccgt gtggcgggcg gctccaccca gctcaaaggc gtggaaagcg atatccagct
     6181 tcccaccgct acggcggcat tcgaactggg agaagacatt ctggactacg cgatgcccta
     6241 tgaccagatt acgccctgca ccaactacaa aaaggactcc tccatcgcgg ccatgctgcc
     6301 cgtgctgaaa gatgccagcg cgaagcgcgt ggaaaaagac cgcgacctcc agattgccag
     6361 ggaagatatc gccatgatga aacagcgcat caaggacaac aagctttccc tgaacaagaa
     6421 aatccgggaa caggaaaact ccgccctgga agaacgccgc aaatccatca acaaggaacg
     6481 taaaatccgc ttcgcggaaa tggccaagga agacgctacc aaatacaaaa tttaccgcct
     6541 gacgctggac gacgtcaacg ccaaggaact gcccctggcg gatccggaaa aagacaatga
     6601 acaattcatg cacctggcgg aagaccccac ggcagaactg gacgactccc cggaataccc
     6661 ctccggcctt gatccggaac tccgcgaagg catcaacatc gtccaggata tgctaaagct
     6721 ggaatcctcc ggaaaataac ggctcgctcc tcgcgaacca ccttgccacc ccggccccat
     6781 tcaggggacc ggggttcttt ttcacggagg caattccggg agccgtgcct cccgaacagc
     6841 ccgaaatgaa tccggggctt gccagccgga gggaaataag gcagtatttt tgcccgtcag
     6901 caatgagcac caccatgaat acgacataca aaattgccat cggcgcggat cacgccggag
     6961 tcgatctcaa ggaaaccgta gcggaactcc tcaaggcatg gggtcatgaa gtgaccgaca
     7021 tgggaaccat gaccaaaaat tcctgtgatt attccgacta cgccaatgca gtcgcaagca
     7081 gcattgctga cggcaccaac gaccgcggca tccttatctg ccggtccggc atcggcatga
     7141 gtattgccgc caaccgctgg gtgggcgtcc gcgccgccct ctgccgcacg gcccctgctg
     7201 cggccctttc ccgccagcac aatgattcca acctgctctg cctggcttcc gcctatgtgg
     7261 acaatgaaag tgtggaagac gtcctggacg cctggctgca tgctgacttt gaaggcggac
     7321 gccatgaacg ccgcgtcgtt aaatcctccg gtagccccct gcaatggacg gacccggaac
     7381 tggcaggcct catccaggag gaaggacgcc gggaaagcaa caacattgaa ctgattgcct
     7441 ccgaaaattt cacttctccc tccgtccggg aagcccaggg ctccctgctg accaataaat
     7501 acgcggaagg ctatcccggc aagcgctggt atggcggttg cgaggtggtg gacaaagtgg
     7561 aacagctcgc cattgaccgt gtgctgaaaa ttttcggcgg ggatcatgcc aacgtgcagc
     7621 cccactccgg ttcccaggcc aacatggccg tttacttctc tgttctgaag ccgggcgaca
     7681 ccattctcac gatggatctc tcccacggcg gccacctcac ccacggacac cgggccaact
     7741 tctccggcaa actgtacaat gtggttcact acggcgtatc ccaggaaacg gaagctattg
     7801 actatgatgc tttggaaaaa ctggctctgg aggtaaagcc gcaaatgatc acggcgggag
     7861 caagcgccta ctcacgcaca atcgactttg aacgcatggg ccagattgcc agggcctgcg
     7921 gtgcctacct gttcgtggac atggcccaca tcgcgggcct ggtggccggg gggcagcacc
     7981 cgaatccggt tccccatgcg gacttcgtct cctccaccac gcataaatcc ctgcgcggcc
     8041 cccgcggcgg cttcgtcatc tgcaaggagg aatacgccaa aaaactggat gcagccgtct
     8101 tccccggaat gcagggcggc cccctcatgc acatcattgc cgccaaggcg gcctgtttcg
     8161 gagaagctct gaagcccgaa ttcaaggact atgcggctca ggttgtcaaa aacgccaagg
     8221 ccatggcggc caaaatggcg gaactcggct tccgcgtcgt ctccaacggt acggacaacc
     8281 acgtattcat ggtagacctc cgcaacaaag gcattaacgg tgcagacgcg caggaagccc
     8341 tggaccgcgt aggcatcacc gtaaacaaga acgccatccc gtttgacacg ggttctccca
     8401 tgaaaccctc cggcatccgc atcggtacgc ctgccgtaac cactcgcggc atgaaggaaa
     8461 aagacgtgga acaggtggct gaattcattg cccgtgcgct ggccctgtac gtaggtgacc
     8521 aggtatgccc gaatccggaa ggcttctccc agcttaagga agaagtttcc gcgtttaacc
     8581 gcaacttccg cctgcctcat taacatctcc tgcgcataaa cgcttttcag gccgttccgc
     8641 ctttcccgcg gaacggcctt ttcatcaaaa gtccgtattc acggcccccg atacgcgcgg
     8701 ccaagacgcc cgcaacaaaa aaaactctta gtcaaggaat ggccggggtt cgtccaaact
     8761 gcacttttcc cctcctatat tgcggacaaa ccggtttgga aatcaactgc cattgtccat
     8821 ccacacttcc tcgttggaac gtctgccctc ttcccggccc aaccggaaaa taaaggttgc
     8881 ggcaggttgg aaataatgga actgatgact tgcatgcgtt gctcctccat ccgcaattaa
     8941 aataatctga aacccttcat tggaaaactg gatatttttt tcatcccaat atccatagct
     9001 agacatgacg gaatacctcc ccatttccat gaatgaaccg tcctgctggc gcagaaaagc
     9061 cttaaattca taactgttaa aattggctcc cctcggcgtt ttccacacta atgcggcttc
     9121 atggtccgcc tgccaaatta ctttgttatc cggattctcc cggaaacagt tttccagaaa
     9181 agtcatctcc tgcttgtgaa aacgcaaggc ggcaacatcc accggaggat gatcgacaag
     9241 gggagaaggc acgtcagaag ccataacaag gggaaccgtg cggcaagaga aaagaagcgt
     9301 tttaagagag gcattcatcc tccagatatt acttcgtcaa aaagacggct caagtctcaa
     9361 tcacctgatc gcctgatgat gcttcagaat gatttatcta tccaaagatc caccgggcca
     9421 gacagacaaa ctatcgcatc cgtctggttt gacaatacag ttccgatact gcttttccgt
     9481 caaaaagtca gttccgccaa tagtgacata aggcgtggga gtagtgcctg ccgttctaaa
     9541 ttcatcataa ggaacagtca ctccaccgct cagaacctca aggagcgcct ttctggcttc
     9601 ttccagtttt tcacagctca gcctgtcaat atccctgtac tcatccactt caaccttggc
     9661 atattgctga tttgttacgg aggcccaatc aggattctta ccgtctttga tttcgccaaa
     9721 ttccgtcaat atactcaaca agggatttcg ataagcattc cctttcgtgc tgccgggacg
     9781 attcaacatc accaatgcac tcgtccttat ctcggcaccc ttcgtatcgg cttccgcata
     9841 gatgactcgc gccattggag agtcataaca tcgtctccgt cgatgaggaa ataatgccgt
     9901 tctgcttttt ctactctaat gtatgctttc caggtgtcag tacgatcttc tacattctgc
     9961 ttgataggaa tcagcacggg gtcggacact caactaacaa acaaattttt acaaaatttg
    10021 atatgtaaaa ttcttttatg gcctgcccgc ggaagtcatg ccgtcccaca cgggaaagta
    10081 ataatgcagg gtaggttcag gctcattcaa caggcgatag tgccaccatt ccggagcata
    10141 attggcaaaa ccctgttttt caaaagccgt tttcagaatc atcctgtttc tgcgttggga
    10201 aggagtcact aatgtagagt ctgtggtgga actgggatcc agcaggtcat gatggccgcc
    10261 catatccacc ggctttccgg tcttggcgta cagcagggaa acatccacag ccacgccccg
    10321 ggaatgttcc gaaaaatcgc gcacatagtg gtctccgaac accttggcct tgtcaatatg
    10381 gggataatac cgggacttca tcttttggtc cccgtcgtca gctccccatt tgcaaatgtc
    10441 cagcacggcg gtatggggac ggtaggcgtc ataaatttcc agcaggtaac cctgacgata
    10501 caaatcttcc gagacttttt tcaacgcctg cgccgcctgg gtgcgcagga tggcgcgccg
    10561 accgtggtaa ccgtccagcg gacgccctac aaaattgtca tacccggcat atttcaaatt
    10621 agtcttcacc agcggagcca cctgatccac atacacaaag cccggctgca tcacgggctg
    10681 aaggggctga ttgaacgccg gtttgaattc cctgctgccg gaacaggaaa ccagtaacat
    10741 gaacatgccg cataccgcag ctacgcaagt cttcatgacc acgaagcgta gcacggcatg
    10801 cggcgcaaat ccggtcagga ttccgtcatt tccaggaatc ccttgatctt gctaatgcgc
    10861 gtgggatggc gcagcttgcg gagggctttg gcttcaatct ggcgaatgcg ttcgcgggtg
    10921 acgctgaact ggcggcccac ctcctccaga gtgcggggac tgccgtcccg gaggccgaag
    10981 cgctgttcaa taacttcgcg ttcacgatcc gtgagggtgt ccagaacatc cttgattttt
    11041 tctttcagaa aagagaagga ggcttcctcc atgggatttt ccgccgcctt gtcttcaata
    11101 aagtcaccaa aggaggtatc gtcggaatcc cccacggggg cttgcaggga aatgggctgc
    11161 tgggccattt tcaaaacgga acgcacgcgt tccacaggca gatggatttc ttccgcgatt
    11221 tcttccggag tgggttccct accatactcc tgcaccagct gcttttgcac gcgcatcaat
    11281 ttattgatgg tttcaatcat gtgcacggga atgcggatgg tgcgggcctg gtctgcaatg
    11341 gagcgggtga tggcctggcg aatccaccag gtggcatagg tggagaattt gtagccgcgg
    11401 cggtattcaa atttttccac cgctttcatc aggcccatgt tgccttcctg aatcagatcc
    11461 aggaaggaga ggccgcggtt ggtatatttt ttggcaatgg aaatgaccag gcgcagattg
    11521 gcctccacca tttcatcctt ggccctgcgg gcttcgcgaa gccatttgcg gagttcctgg
    11581 taggcttctc ccatctcatc cgcagtcatc cacatgcgaa gctccagctc tgccagatgt
    11641 tccttgagtt ccttgttgtc cggttccagg gcaaccttct tctgcatgcg gagcacgcgg
    11701 tcgcgggcct cgtcaatcat ggaacagaaa tcctcaatca ccttgtgctt gaaatacagg
    11761 cgaccgaaga acttcaccaa aacgccggca actttgtcga attcggcctg gcgggccttg
    11821 acggacttgg ccgcctcctt gctgcggtac agcttgcgga aggaaccgga tgcgtcctga
    11881 tgggtctgct gtatctgggc gcagagctgg gccagccctt tcatatagcg ctcacgggag
    11941 tctatctttt tgtcaaggat aacgcggtca aagcgttcct cattgtcgag caggcgatag
    12001 gctacgtcca gataggcatt cgcgatgaaa ccgaagcggt gcacacagcg ctggacgttc
    12061 tgttccgcgt cctcaattct cttggaaatg gccacttcct cttcacgggt aagcagggaa
    12121 acctggccca tttgtttcag gtacatgcgg acgggatcat ccagaatgtc catcttggct
    12181 tcgagctttt cctcttcgtc ctcttcttcc gtgctgatgc gggtgcggtc cgtgtagctg
    12241 tccacatcag acgcgtcaat gatgtcaaac gccatgctgc gcaggcggtc catgatggcc
    12301 tcgtaatcct gctgatcgac aatgtcgttg ggaagggagt cattaatatc gtcaaaagtc
    12361 agatacccct gttccttggc gagcttgata agttcacgga ttttttcctg aatttccgga
    12421 gcgtcaatcg gattcgtgat cttgcggctg gcagccctct tgcgttcctc ttccagtatt
    12481 tcgcttaatt ttttgggcgt ttcctccttc gcggcaggtt tggcggcctt cttctcagcc
    12541 ggcttcttct ccgcagcctt ggctggagct ttcttggcag ggacagcttc aggcgccttt
    12601 gccggagtct ttttggcgga cggcgccgcc ttcttggagg cagaagccgg agacgctttt
    12661 tttgccgggg cctttttttc tgcaacgggc ttggcggcgg ccttttccgc agccggagcg
    12721 gcagccttgg cagagttttt tttgacagga gccgccttcg gagcggtttt ggaatttttt
    12781 gcggcgggcc gggccgctat tttttccttg gattcagaag cttttttgtt ggcggaagtc
    12841 ttcttgggag aagaggggga tgggtcaccg gatttggaca tatcttgaaa agaagagggc
    12901 acaattcacc attatatcaa tacccggagg tatcgaggca ctacattgta cccagcaaac
    12961 gcttcaagtc aacactaagt ttggacaatt ccagccttct ggcagcatcc gtggagggat
    13021 ccgccagttc cgccatcagg gaggcgatgt gcctctccaa agcggccttc gcaatgccgg
    13081 agcaggcttc ctgtacgctc ctgtccacat cgggaatagg tatgggctcc agattcagca
    13141 tgcccagggc atcccgttcc ggctgaggca gagacaccag aaaagcctga acggccgcgg
    13201 ggctgtccgg agaaggcagc ctggccagaa ttttttttaa gatgcctccg ccctgcagca
    13261 gccgcatcgg ctccagcaat tcttcaatgc ggtccacgat aagcccctgg gcacgggaat
    13321 tctgaagggc cagttcacag agcacggcca cggcgcgatc cattttcaca ggaggatgct
    13381 gcggaacctc ctcagccgtc ttacgctctg cggactgggt ctgttcctgc cccttccggc
    13441 gtccggccat tctgacggcg ccgcgcaaat tatccgcagc catgcgcatg cgcgtggcta
    13501 cgtcagccgt agccaggtcc cgggccaccg ggtcgctcat gcaggccagc agggccgtca
    13561 tctcacgggc aaaagcggcg ctggaagcgg cgtcatccaa caaaccgctc tccctggccc
    13621 gttcaatgcg aacctcaaag aaggaacggg cccgctccac catcccagaa aaagcctctt
    13681 tcccctccct cttcatgaaa gaatccggat cgtccccggc aggaaggttc accaggtaaa
    13741 tatccttgcc aaaaggggcc agttcacgga aagcccggtc cgccgcagcc atgcccgcct
    13801 tgtccgcgtc aaaacacaac actatccgtg aagcatagcg tgaaagaatc cgggcatgct
    13861 ccggagtaaa cgccgtcccc aaaggggcta cggcaaaaga aatacccgcc tcgtggcagg
    13921 caatgacgtc tatctgcccc tcgcacaaca gggctttgcc gctttttccc atagggccgc
    13981 gcgccttgtc cagggcaaac accacgtccc cctttttgaa aatggccgtc tccggagaat
    14041 taacgtactt gcctacgttt tcctgaggcc gcagaatgcg gccggaaaac gccacacatt
    14101 ctccgtacac attgttgatg gggaacatca gcctgtcccg gaagcgggcg tacaaatctc
    14161 cccgttctcc gcggttggcg agaccggaat ctatcagcac ctggtccgga atcccctccc
    14221 tgcgggccca ggccagaaac tcccgcgagg agtcaggagc ccaccctacc tgccagcgtt
    14281 tcgccatgtc tgccccgtat ccgcgggaat tcaggtaagc cctggcctcc gccgccttcg
    14341 gggaacaaag caggagcctg tggaaaaacc cagccgcctt cttgtgggcc accagcaaag
    14401 ccgtgcgtgc cttgcgccgc ctttcctccg ctgcgtcata gacctcctcc acaatggaga
    14461 ttccggcttt ttccgccagc cttcgcacgg tatccgcaaa ccccaggttc tccatcttca
    14521 tcacaaaagt gatggcagac cccccttccc cgcagccaaa gcatttaaaa ttctgccgac
    14581 tctggtccac aatgaaggaa ggcgtctttt cattgtggaa cgggcagcat gccttccacc
    14641 gggaaccggc ccttttcagg ggaagatagg atcccaccac atctacaatg tcagtggcct
    14701 ccagaacgcg ccggatactg tcttcatcaa tccttcccat aaggcggaac cagtctatca
    14761 caaggggtac gggtgcacaa gaatacagaa tcggaacgcc atgcgaggaa acaaggcctc
    14821 cacaggcagg gaaaagacgg cgcagcaccg gcggcttccc gccccggagg aacacatttc
    14881 cccattgagc gcgggagcgg aacatgctag cctgtccgca tgacgcgcat tcaccttctg
    14941 ttgctggctt tgctggccct gtttgccggc gcccccttcc gggcggccgc ggaggaaacc
    15001 ttccgcgaca gggtagtcgt catccccgta ggggaggact ccctgaccag caaacaaagc
    15061 ttcggcttca tgaaccgcat cctgaaaaga gcgcaggagg agcaggcgcg cgcggtaata
    15121 ttcgatttga acacgcccgg cggcctggcc tgggagacca gcgagatgat gatgaaaagc
    15181 atccagccgc tcaccattcc cacctacgcc tacgtaaacc ccagggccat gtccgccggg
    15241 gcgctcatct ccgcggcgtg cgacaaaatc tacatggctc ccgtctcctc catcggcgca
    15301 gcgggcatca tcacctcatc cggagaggaa atggatcccg tcatgcgcaa gaaagcggaa
    15361 agcgccttct gggcctttac ccgctccgtc gtgacggaaa agggttaccg cccggaagtg
    15421 gtaaaagcca tgatgatccc gtccgaccag gaacagcagt tcggccccgt caaactggaa
    15481 aaaggcgcgc tcctgaccct gacgggcaag gaagccgccg cgcgtctgga agacggcagg
    15541 cccctgctgg cccagggcac agcctcctcc gtcacggacc tgctggccca ggagaaagta
    15601 aatgcccccg tggtcacggc ggaacctaca gcctttgagc gcatcggcct ctggattgca
    15661 tgggcttccc ccatcctgat cctgctgggc atcggcggca tttacatgga atttaaaacg
    15721 ccgggattcg gcatcggcgg cattgtggcc attgccgcgt ttgccctgtt cttcttcggc
    15781 aacaacattg ccgggaatct ggccggctat gagacggcgg ccctgctcgt tctgggcata
    15841 gtcctccttt gcgtggaatt cttcgttatc ccgggcacat tcattgcggc cattgcgggc
    15901 ggcgtctgca tctttctggc cctgttcggc ggcatggtca gctcctggga atgggaccat
    15961 atcatccggg acggacagtg ggatgacgtc aacacgctgg cggcagtcct gggcatcccc
    16021 cttctgaaac tggccctggg cctgaccgga ggctcccttc tgattgccct gctgatgaaa
    16081 tacctcccgg attccatcct gatgcgccgc gttaccaacg ccacggtcag cggaggttcc
    16141 gccggggaag cggtcaatat cgcgcgcgtt caggtaggag accgcgggaa cgccgtcacg
    16201 gagctgaaac ccaatggaaa agccatgatt catggcgaaa tatgcgaggt tttctctcga
    16261 caaggcatac tgattaaggg tacccctgtg cgcgtcgtgg acatccggcc attcgacctc
    16321 gtcgtcgtca aggacgactc cccctccggg gattgacgcc atcacgcttc ttatctactg
    16381 ccatgcctct caccgttgga ctcatttccc tgggctgccc caagaatctg gttgactctg
    16441 aaatcatgat cggccacctg caaaaagccg gcatgaccat gactccggaa ccggaactcg
    16501 cggacgtaat ggtggtcaat acctgcgctt tcattgacca ggccaagcag gaagccatcg
    16561 atgccattct ggacatcgtg cgtgcccggg aaaatggggc ttatccggaa aaccagaaac
    16621 tcatcgtggc gggctgcctg tcccagcgct tccggaagga actgcccgcc ctgcttccgg
    16681 aggtggacgc ctttatcggt ccggaccaaa tcaccaaact gccggaaatc atcactcaag
    16741 tgatggaccg gaccatacag gaccggaact tcattgaagg aaaatgcagg tacgttcctg
    16801 actggaacac gccgcgctac cgccttaccc cgccgcacac ggcctacatc aagatagcgg
    16861 aaggctgcaa ccacggctgc gcctactgca tcattcccat gatccgcgga cgccaccgca
    16921 gccgttccca gcaggacgta gtgcgtgaag cggaaaccct tatccggtcc ggcgtgaagg
    16981 aaatctgcct catcgcgcag gacatcacct attacggcat ggataaatgg acggatgcgc
    17041 gcccaaaccg ccgcagcgcg gtggattcct cccgcggaga atccctggct tccctgctcc
    17101 gcgctctgaa cgccattgag ggagaattct ggatcaggct gctctacacc caccccgccc
    17161 actggagcga cgaactgacc gcagccatcg cggaatgccc caaagtggcc cgctatgtag
    17221 acattcccct ccagcatatt tccgataata tgctggacgc catgcagcgc gtaacggacg
    17281 gaaactatat ccgcacgttg ctccggaata tccggaaggc cgttccgggc atcgccatcc
    17341 gcaccacctt catcaccgga ttccccggag aaacggaaga cgaccatcag gaactgatgg
    17401 agtttatcga ggaattccgt tttgaacgcg ccggcatttt caccttctca cgggaggaag
    17461 ggacaaaagc ctacaagatg cccaaccagg tgcaccaccg cactaaggct cgccgctaca
    17521 atgaagccac cctgttgctg gcgcgtctcg cttcggaaac gggccaggaa caaatcggca
    17581 ggcaaatacg cgtgctggtg gatgctcccg gagtcgccag aacggaatgg gatgctccgg
    17641 acattgacgg aaccgtctcc gttcctctga cgctgcccgt cgggcaattc gccactgtca
    17701 ccgtaacgga tgccgttgcg tatgaattga cggcggaata aaattgcttc cgtgcctgtc
    17761 ggaaagaacc cgggaacaaa aatacaggcg aagcgcaggc cgacgtatat tgcctcctgt
    17821 tttccgcccg gctttccgga ttttctgcaa aggccctact gcttcacgct ggattcaggc
    17881 aggggaaggt tttccggagg agcttcctga gacgcagctc ccgacggaga aacggaattc
    17941 ggaacaggaa ccagagaggt attcatccgg gatatatcca gctcacggga agcggcggct
    18001 tccgggctga cggcgcccat gtttgagggc aaaggaagca aagctccgtc cggaccaatt
    18061 agatcggaag cggaaacagg ataaaaaccc atcatttcct gcggattaac aatctccgag
    18121 aggcttacgt aaccctggct gccgtcttca aattcgactt tggcgaacaa atccccgcag
    18181 accagcacct tcccggtttt caccccaccc ctgacggttc cgcttgtgga aaaattactt
    18241 tccggattct gttcaaacac catcattctg tccttcttga acgtaacggc ttccccaggt
    18301 ttgtaaaatg cctgctccgc cttgttttta gccgccattt tcttggaagt gattcccacg
    18361 ggatccaacg cagaactctg gcggtcccag gcattcacat gcttgttcgg accgcaggag
    18421 gccagcagca ccagcaaagc ggaagggaga aaaagcaatt tcatttgcag attatcttaa
    18481 ttgccgggga ttacggatgg aagcagaatt tgcgcctgtt tccgccagag actgtttcta
    18541 aaagaaaaat tccgcctttc cctccggttc atgctgtttc ccgcagagaa aaccatgccg
    18601 cttccgaact gcatcccgga caaataatcg gattcccggt aaggcgtcta ttcctgcggc
    18661 ggaaaaaaac agcctgtctt accgtccccc tgctccactt cccaggaaaa accgcaaagc
    18721 atggcgcgga gacaacgtat taagcttgcc gccttcctgc cggaaatgct attctggtgc
    18781 ttactgttcc aacggctgac gcaggtccat ccgggggaac cgctcaagta atgcgttctg
    18841 atatggatga gaaaagatta gccggcgtag ttctgccatt gttctccctg cgccgtaaca
    18901 atgaccacgg aatcggggac ctgacggcct tgcgccaatg gattgactgg gcggcggatg
    18961 cccatgtggg gttcctccag ctgctccccg tcaatgccct gggcagggac gaatgcccct
    19021 ccccctattc cgccatcagc tccgtagctt tggaacccct gtacctttcc ctggaaccct
    19081 ggaccatacc cggcctggaa gaacgcgtat tcaatgaaac gggggacacg ctgccgtggg
    19141 aacagccctc cggcccggat ctggtggact accccaaggt cagattctgg aaaatgtgga
    19201 ttctgcgggg agcctggaac aatttcaaaa ccaaaccgga atatgagtgc attatgccca
    19261 aattcaggga atgggtgaaa gaacagggaa gctggctgga agactttgtc tgtttccagg
    19321 ttctctgcga cctgttcggc acggaaatct ggtggcactg gccggaacag gatccggcgc
    19381 gggccaaggc cattgccgcc gattacgagg aagaaaagga ttttgcccgc tggctccagt
    19441 ggctctgtga aaaacagtgg gaattcatcc gtatttatgc ggacgaacgc aatgtgaagc
    19501 tgatggggga tatccccatt ggcgtttccc tttccagcgc cgacgtcttt tttgaacgcc
    19561 acctgtttga cacggaatgg tgcggaggag ccccggcgga aggcagctat gcggaagacc
    19621 cattcacggc caaatggggg caaaactggg gcattccgct gtatagatgg gatgtcatgg
    19681 cgcaggacaa cttcgcctgg tggcgccgcc gggtaaaata ctgcaccaag attttcagca
    19741 tgtacaggat cgaccacatc ctgggctttt accgcattta ctccttcccg tggaaaccca
    19801 cggaaaacgg ggtattcctg ccgctttccc ccgatcaggc ggcccagaga acgggcggaa
    19861 ggctgcccgg cttcaagccg cgcggggatg acaatgctgc ggaccgcaac atgaacctgg
    19921 cggacgggga cctgtacctg cgccttctgc tttccgccgc tcccggcgtc agcgtggtgg
    19981 gggaagacct gggctgcgtt ccggactacg tgcgcccgaa catgcgccag ctggatatcc
    20041 ccggtttcaa aattccccac tgggaaatca aggcggacgg aaccattaca tctggaaaag
    20101 agtatcacga atgctccttt gcggcgttcg gaacgcatga tttcgaaacc atcatgcaga
    20161 catggaacga cagctacgcg aaaatcgaac acgcccgcaa gctgggcctg tgggagaacg
    20221 gctcccccaa aacgccgtcc agcccggaac aggaaaacat cgtgcgccag gcggaggacg
    20281 gagcgcgcct tctgaaatgg tttgcggatt tctccggcat gcagcctgaa acctgtcttt
    20341 cctactggaa ccaggaaatc aagacggcca tgtacaacgc cctgttccgt tcccgttccc
    20401 gttatgcggc cattctctgg cctgctctct tcggcatcaa caaacggctc aatattcccg
    20461 gcactacggg agggaccaac tggagggaac gcatgccctt caaggccgtg gaagcatgcg
    20521 gcatgccgca gaccgcatgg ctgcgcacca tcatcgacga atccggccgc acgccgctgc
    20581 agggtgaaga cgccatcagg gcgctcaagg aatccagcaa acgcctcttc ccgaagatca
    20641 ccgtcaacaa cgaacgcttc ctgaaacgga tgctgatgtg gccgaacggc aaaacattgt
    20701 agctgtcccc ccctgccgga acatcccccc cgcagtatca aaaaagggcg cgtttccttc
    20761 gaaaacgtgc ccttttttct atccgtgctg atgtttccta aaacgccctg gatacacccg
    20821 catttacgcg gtggtacacg gagcttcccc gggcttccat gccataaccg gcataaaccg
    20881 tccagcggtc attccactgc aaggcggcat catactccac gcgtacggca tggcgggctg
    20941 gttggacacc acgggccctc cagaaaatgt cgctgtatat gctgtaaacg gaagcctccg
    21001 gcacatcctg aatagcatct cccacatagg aaaggcggag cacctgcgtc cagtgcctgc
    21061 ctttccagtc accacttctc tcaacactca cccctgccgg gatggacaac cggcgcaaaa
    21121 ccgctccttc aaattttctg gcatagctgc cgtcttcaag aaaagaagcc tccgtgccat
    21181 gtgtaaactc catcctcaaa aacggagaca cagtccatcc tcctgcatac tgaatgcttc
    21241 tggaaacttc cgcctggacc agccacgttc tattgttcca ttccccatga gcgtccatac
    21301 cgttgctgaa ccggcttttc atgcagttat cagtcattcc ccaagtaaga tcggctttca
    21361 ccgtccacag ggaacgctcg ccggagcgga atatccgccc ccaataaatg ctccccatca
    21421 gggaatcctg ggtaatcctc ccattaaaat cccggctcct gctgaaaccg tgcatttgtc
    21481 cgaacgctat cccccatatc ccgctctttc ctccaaaatc ggaatccatg ccaacggaat
    21541 atccgccccc gtcatagtca taaccatcta cgccgccccg ggaacgctgg cgggcgaaat
    21601 cccccagccc cattccccat acattgcgtg cataccgctg atacaaccgc accggggtta
    21661 actgcgccaa cgcagtattc ccaagagaag ccgcgtttga agcggaactc caaagggaat
    21721 tgagagcaag ctctccagcc tgctccggac ggacctgatc cgggcggcat ccggcttcat
    21781 gtttccaaat tgccaacagc tggtcatctt ctccatctcc atccatgtcg cgccattcat
    21841 attcccaggt accgccgtag gtatagggag aatcaaccgt ggatgactgg gtcgtattgg
    21901 aaataacccc tttcagctct ccttgcggcc gtttgctgga agaatccata ttaaaaatga
    21961 caaactcccg gtcccttccc caggcggcgg aggcgtaatc atccagcgta tccgcaattc
    22021 caatggttcc ccccagtaaa aattccccgg aggcggtgat gttgataccg ctgtcccccc
    22081 gttccgagaa aggcatgaga tcccagtcaa acccgtggga catgtcaatg gtgacagcgg
    22141 aaagggaagg cagttcatag gtcttcccct cccaactgtc ggaaccgatg ggagcggctc
    22201 ccacccgcag gatagcgccg ctgccatggc cggaaacaat cacctgctgg gcgctgatgg
    22261 atgaaccgga cgtcatttcc agcactccgc cggccaggtt aaacaccggt ttgtcctgct
    22321 ccactcttga acttccctgc catacatccc cactctcatt cgccaggttc atgccgtatt
    22381 caagaaccag ccgtccccct tccacaataa tatttgattc aagtttcacc ctgcgggagg
    22441 tctccacccg ttccgcgtaa tcaccatcac tttccccgtc attgcgaact agaaacgact
    22501 ccgtcaatgc accggaaaaa cgcacggttc ctccaaactc cacaggaata cctccataat
    22561 ctgcccaatc ggccggaatt ctgttaatat gcagggtagg catggctaca gtaatacttt
    22621 caatatcaat actatcataa aatacaattt ctcgtccggc ctgggcgcgg aaatccatgg
    22681 ttaaattccc attgggctga gatatgcccg tataatcgtt ttcgggagca taagcaaaaa
    22741 tccccagggc gcgggcaaag acccccgaac ttcctttaac caagttgcct cggaaaacga
    22801 tattcccgcc atccgcagac aaaagccatt gccctccaaa ccttttccct tcattattgc
    22861 cgcaataaat ggctccggcc gtatttttat cagtcacatt attttcaaac aggacatcac
    22921 ccgtttgcac cagcttaaaa gatccataaa tagtgatagc gcccgccatc cccggcatgc
    22981 tccctccgga agccttatta tttctaaaaa taatatctcc ccggatgttg ctaaacgaca
    23041 aagacggctg gttatccggt aagaatatgg atccaatgcc tatcgccgca ggtcctccgg
    23101 tggacgtgtt attttcaaac aggatatttt ccgtatcatt aaatgaaata ctcccttcat
    23161 tggtaaaaat ccccgcagaa ctcatgtaat aagaagtttt aacaagattc ctgaagataa
    23221 tacttcccgt atggtcaaaa gaaatacgga actatatact ttaatcttgt aaatcctcca
    23281 taatccatta cactactcct agaaaaagaa aggagtagaa cgtgacatac cgcgaattca
    23341 ttgataaatt tcctgatgat gagagtgtgg caaaatatct cattgaaaaa cggtttgagg
    23401 gaaaaatagt ttgcccattt tgcgggaaaa cggagaaggt ttatcacgct cactataatt
    23461 gccgcaatgc ctattgcaac aattgtaaga tggaattctc tattttcaag ggaaccattt
    23521 ttaaggaaac aagaatgcct ttaagacatt ggctgtatgc tatcaatatg gtttgtttgt
    23581 ctaaaaaagg gatttcagcc atgcaattga aaagggaatt aggtgtttcc tataaaaccg
    23641 cttggagaat gatgcacaag attagaagtg ctatggcaaa aagagaggta ggagaaactt
    23701 ttgaggcgat tgtagaaatt gatgaaacct atgttggagg aaaaccccgg aagaagaatg
    23761 agcatgggga tgatgacgac ttgccgaaaa atcctagagg aagagggacg gctaaagttc
    23821 ctgttattgg agtcagagaa aggggaactg ggaaagttca tgccgtcgtt gccaatagga
    23881 atgaagaagg aaaacaattg acgggcaaac aattgttcaa tgtccttagc aaggtttgca
    23941 aacctaatac caaagtcatg actgaccagt ttaagggcta caatattcta aattatccta
    24001 acaataagaa tcttacccgc atcatgattg accataacgt tatgttcagc gcggggaacg
    24061 gggttcatac caatggaatt gaaagcttct gggccttact gaaaagaggt attcatggga
    24121 tatttcacca tgtttctctt aaacacctac agaaatatgt ggatgaattc tgcttcaggc
    24181 aatgttatag gaacgagaat gaagcttttg aagttttgat gggcttatgc tggaaatgaa
    24241 aagccgccac cttgcgagca aggcgacggc cagtcatgcg attccaataa aaactattca
    24301 aatttttctt agattatcaa tcttcctcca ttctggttta ggattcaata ctacgaataa
    24361 atgagcctga ttcttttgtt tgttcttgtt ttgggccaaa cgacgaccgg gtacgagttg
    24421 agcttgaacc tggtctggac ttaagacata taagcctcct gtactcgcat caacggccaa
    24481 tccaatgatg cctcccataa gaagatttcc ccatacccag ccggaaacac tatgagttag
    24541 agttgctgaa tagggataat aacccggaag agttattcta actgtgtggt cttcatccct
    24601 ttccaattct atagttatcg gagtccttcc ccgtggcata ccatcaatgg aaacggtggc
    24661 tccatctgga gaacttgaaa aaccgacgct ttgtttgctt ccattaacaa tagaaccaca
    24721 agaaattatc aacagtggag ataaaagagc acagatagaa cagagagatt tcatagctat
    24781 taaagtggtt taaggattat aatgcgcccc tctccgaaga gagggacgcc agttaattca
    24841 ccgccgaaga ggtctagaag gtccagctaa taccagcttg gatgacatgg taagggtcag
    24901 tatcatggaa tttgagaagt tcgtacccta aaatcaaatc tgttgtattg ccaatggcaa
    24961 agcggaatcc taacgtagca gagaatgtaa aatctgcatt gaactcagtt gcactagctc
    25021 tacctctctc tattcgataa gtagaatcat attgccaaat ttttctatca atcgttgctg
    25081 tagctttcaa agaattgaaa gttacgccaa ttttgccacc taagaagaaa tatgctttct
    25141 cctgaaccaa aggaagattc aatgtatatc ctgccagtag ggggatagat gtttggtatt
    25201 tgattttcaa gtcaacattc gtaatgtttt tctcatttag aaaatgttga atttgagcat
    25261 cggaatatcc ccattcacgt ccaatatcga aggcattgaa atgtttagca tcggaagaca
    25321 tcaatcccgc cgtcaaagac aattcgtgaa cgatatctcc tgtttcagta tatgaattta
    25381 ggctgagcaa accgccgcca agattaggca tgtcgctatc tcctgcaatc ccataggccc
    25441 ctttgatagt cattccaaag gtagaatcgt atttctgcgt ataggacgga gaactgtaag
    25501 cccccccggc tctataatag ttccaagggt cagacgcttg gaccgaaaaa gcacaggtca
    25561 atagtcctaa cgcacacgtt ataaataagt ttatcttcat agtatcttgt ttgtaggtta
    25621 tagattattt ataataggat tcgtattttt tttcttctta cttttttctt ctgcgcgaag
    25681 aacttgctct cgttcggtcc acgcaaattt ttctaagcga gtgtctgtaa tagtcaattt
    25741 accgagttgt gctcccgctt gaggccaata ccaattagaa tattcagcga taatgttaga
    25801 agatttaact gaatatttat cttccctatt gtgtttgtcc tttgagaacc tgatgccttc
    25861 acagaattgc tgaataaact gtatggcttc ttcttttttc agtatgtcgt tatcccaccc
    25921 cttttcgaca ataattttcc agcataaata ctttttggga gtaaagaaga cggtgatatt
    25981 atacttcgcc tttttccatt tgatggtagg gaagccctca ttgttttttc ccctctctcc
    26041 ttctctccaa cgttttgtga gttctgatgg agtttcccca agacgagtac tctcattggc
    26101 atgagaagtt gtaatgagaa aaagtgcact aaaaatgctc ccaatgagta tgtttttctt
    26161 catggttggt cgtttatgag ttgaagtcat aatgaacaca cggtcatcaa cgaaatgctc
    26221 taattttgcg tctaattgcg aatcgcaata atcatcatat gcgagttatt agcataacaa
    26281 atgcgaacaa cacaagctct ttctttctat gaactgggtt ctatcagata atcaaaccgc
    26341 atgtttacga gcaattccaa gcctgaaccg gactatgtgg acactaaaaa attaggccaa
    26401 tggctcagcg aacattccat tagccgtgcc gaaatagcca aggctcttgg agtccaaacc
    26461 accgtggttc ataactggtt tgctcgtcaa agaatcccta aaaacgtgca aggctcattg
    26521 cggcatttga tgagtaagga ctatccttcg gaattggaat ctcaaatcgc tgttcgaatg
    26581 aaaaattcca ctctgaacga ggttgtcaaa cgggctgtca aggaagggct gtccgtggag
    26641 gaatggattt tgagggcggt ggaagagagg ttaggacgga gtaaatccct acaataaggg
    26701 atgttagaaa caactttttt tatttgttag aaagtcattt gcgacataag gaaataccac
    26761 tggcatcaaa tgaatgaata acattcaata agagtcaaat ccaacaggaa tgacagagac
    26821 aaaatgccat caaatgaaat tgttaaaaag tgtaatgttt tagagagcaa acattctaaa
    26881 cgtcatgaaa ataacaggaa aagcttgacc agatttgaat gcaatacata attttgcgac
    26941 tacattttcg ttttttatga tttcatacaa tttcatacat tagccagaaa ttctagccac
    27001 tatgttcgaa gaacctaaaa atgaactgga gaagtacagg gttggaatga ttgtggccct
    27061 tgataataat ataagtgttg atgggttcaa aaatccagat gcccaacaca cggtctttat
    27121 tgatgaagaa tttattaaaa gggcaaaatc tgaagtgtgt attttatgta atcggttgag
    27181 ccaagatgtt tggtgttcag aaggtgtatt aaattctctc gagatagcta aaaaaaatgg
    27241 agtttgtgtg aaattaatca ctcgtaagcc tgtggatgga acaaaaaatg gtaataaat
//
LOCUS       DAWEMX010000014        27248 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_727_length_27248_cov_13.708896, whole genome shotgun sequence.
ACCESSION   DAWEMX010000014 DAWEMX010000000
VERSION     DAWEMX010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 27248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27248
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_727_length_27248_cov_13.708896"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7342..7351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6104 a         8295 c         7166 g         5673 t
ORIGIN      
        1 gacccagtat atgcggcatg tacattccca cacaggaaga acggtatgca aaaaaagggg
       61 ttgtttccct catcctgtgc gttgtagtcc cttcctgcct gtatgtctct cttctgtcca
      121 agcagctgat gatggtacgc gggcatgaat gggaagctgt catgaaactc tttctggcat
      181 tcacctaccc gctggcggcc ctcttcgcca tgacgggcat tccgttcttc tggtggtctg
      241 tgggcatctc tctggcggtt catgccggca tcgtcgcctg gctgaaaaca acgcctctcc
      301 tgtcccctaa aaaagccgtc tgcatcgccc tgtgcctggg gatgctggac tttctgttcg
      361 tgaaactatt tccttttccg gcacaaatca tgggataagc ctgccctgga aaatatctgg
      421 gatattccat cctccggttc aggactgctt taactggctc gagaatcttc ttaatctcac
      481 gcctcgttgt ttggcatcag tatcgccctc ctcatacgcg aaacacagtg ccccggaacc
      541 atcagcggcg gccaacaccg gcttgggata aaactttcct ttctccagaa cctccttcac
      601 agaatcagct aacaaggatt ttccatcctt taccggcaga caaaccagga cgtactcaat
      661 gcctccccct tctaatccgg agtaaatgcg cctttgggga accagacatc cgcccatgaa
      721 tggaaaatgc cgccagaaag cagacaccca acagggcagc taactttcct atgagttctc
      781 accacaggcg cttcccacaa gcgcgtcaaa ggtaaaaact cctgtttaat cttgaacgcc
      841 agagcctggt atggcagagt gcgtccgccc attcgttgcg cctgtggcga aattggtaga
      901 cgcgccagac ttaggatctg gtgccgaaag gcgtacaggt tcgagtcctg ccaggcgcac
      961 catttcacaa tataggcata tcattaacag tgattaccta tatttcttga ggaaaggggc
     1021 aatgaatgcc gtccccagtc caaccagggc aatcagggag aaagaccacc ggagggagga
     1081 agcatgggca atatgaccaa tgaccggcgg ccccatcagg aaccccagaa agccgatggt
     1141 agaaacggaa gccagcgcca tgctgggaat cattttccgg gatttcccgg ccatgctgta
     1201 gcagagcggg acaatggaag aggtgccaaa acccaccagc agaaagccca gcgtggcgga
     1261 ccacagcatc gggaacagga cggagacgag caatccggag gcaataagga tgccgctggc
     1321 gcgcagaacc cgcacaggcc cgaagcgcat caccaggcgg tctgccgtaa aacgccccag
     1381 ggccatggtg ctcatgaatg ccacgtatcc catctgcacc agccccggac cgggttttac
     1441 cacgctttca aagtacacgc cgctccagtc aaacatggtt ccttcgctca ccatgcttcc
     1501 gaaggcgatc agcccgatga ccagtacata agcatccatg ctccggaaca tgccgcctcc
     1561 ctgggaggag gaacgccggg catccctcgg cagaagagag ggggaaaaaa cggccagaat
     1621 aatcatgcat atcaggaaaa tggcgatgaa atgcgtttcc gcagaaatat gccagtccac
     1681 catggcagct cccagcaaag ctccgaaaaa acccgccagg ctccataaac cgtggaaccg
     1741 ggccatgatg ctgcactggt acagccgttc cactcccact ccctgcgtat tgacggatat
     1801 attcgtcaga tttgcggcta ccccaaagaa aaataacccg gccgccagtt cccagaaaga
     1861 ccccgccata cccaggcata caagcacggc aggataaaag acggatgccg ccatcagtat
     1921 tttccggctg ccgcaacggc caaccaggta accggatagc gccatggcag acatctgccc
     1981 caccgggacg gcaaagagaa cggaccccaa gtcggcatcg ttcaacccca atgcactttt
     2041 gatgtccggg atgcgggatg cccaactggc gaacaccaaa ccctgaagaa aataaaaggt
     2101 actgaccgac agacggtaaa caccttgcgt cgttttagga agaggcacat tcatgatgaa
     2161 tttcctgatc gcgcaaacgc tatccctatt cccctcttct ggcaagagaa atcatctttt
     2221 gtacaataag attttacacg caaacaggca tccggaaaaa ggacaggatt tacggatatt
     2281 tctcccccgc cattttccaa ggcaattttt ctttcacata aaaaacgccg tattccttca
     2341 tggaacacgg cgttggtaaa agggaacaaa aacggtcaac ggggatcgtg gtgccagttc
     2401 agggcaccct tgcccagcgc atacaggtag gccaccagca aaatgccaat gaaaatgcac
     2461 atggccgtga acgattccgg attcatcata atatagtccc ggaaattgac ggcccagggg
     2521 tagaggaata ccacttccaa gtcaaaaatg acaaagagca tggccaccat atagaatttt
     2581 acagagaaca tgggggcgcc ttcccccatg ggaatcatcc cgcattcgta gggaatgtcc
     2641 gccggtttac gcacggaaga acgcttgccc atcaagatac tgagcaacaa gatcatgccg
     2701 gccagcccca gggcggccgc cacctgcacg agcactgaaa aaaattcctg attcatgacg
     2761 tctggcacgg gtgtaccaca gacattacgg aaaagcaaaa ctaaaaagga caacagcttt
     2821 catgctgacg gaagggccgg taaaattccg tctcctgccc tgtcagacag tacaaaggca
     2881 acagtccgtt ccatggggac gcccggcatt tcacatccct gggacttcct ttctgcgggg
     2941 ctctgtcttc cagggcatct tacggcaaac gtctgtttat cacaaaactc cgctatccgg
     3001 ttttaacacg cagaatccag gcaggatgca gccttccact tcagcattcg gcaatgaacc
     3061 ggcaacaaca gcagccggca atggcccagc tatcagaaag ccctcccctc acggaaagcg
     3121 tacaggaatc cccttcattg ccgggaataa cggactcacg cccccatcaa atgggaaatc
     3181 cactttccgg ccgcttccat cagaataata caccccgcca atattccgaa aaaaattccc
     3241 gccaatttaa gcagcagaaa gcccattccc agaaaaacac cgccaatgca agcgaacacg
     3301 gccatcgcca gcatattaaa aaaatctgac aataacatca acataaccct gattttattc
     3361 cattacacgg caattagaaa cggggtttcc tgcaattccc ggccctttcc tgcggaaaat
     3421 gggcgtccaa catctttcca agcacctgca tcactccgtt ttcctcattg gacggggcct
     3481 caaatgcagc catggaacgg atttccggca cggcattcgc catggcataa ctgaaacgcg
     3541 cctgccacat cattggagcg tcattcatct gatccccgaa aaccatcgtc tcctccggag
     3601 aaactcccag tttccgctga atcaattcca gggcggcccc cttattggcc cctttccggc
     3661 tgatatccat ccatttctct ccggagacag ccacctggaa gccatgttcc aaatgcttca
     3721 gccggggata gctgttttcc ccggctccca ggaagtcata tactgctatt ttcaaaagtt
     3781 tgtccccccg cacgtccagc aggtcaccca cttcttgaca gcgtgtgtaa tatttgcggg
     3841 cctcccggac gaattccggc tgtttgtcct caatatatgc ggaatcccgc cctgcgagga
     3901 cgatgcaggt tccttcaatg cgcctgatcc cggcaatcaa accggctacc gtttcatgat
     3961 ccatgtccag aagcagccat tcttttctac ccgccgccgc ataagcgcca ttctcggcaa
     4021 taaacagcat ccggtccgcc acgggggcaa aggtccggag caatccgtca tactggcggc
     4081 cgcttgctgc tgcaaaacga atgccgcgaa gctcaagttc cctcaacaag ggaaaaaacg
     4141 cagggtccaa ttcacccttt ccattaagca gcgtcccatc catatcggac gcaataagtt
     4201 tgacggaatc cataatgctg ccagaatcat cgtttatcct tacttccagg gcaagagcgg
     4261 cttcagacag catggagcgg acagcgaaag gaaagaccag gaggccagca gcagggatac
     4321 agttctctcc atcattttac gggagaatct ccatttcttc cataagtttc agcttccttc
     4381 attctaccgt gagtgcagca gcggcaaatg caaataattt gaaaccatcc gcacatccgc
     4441 aatatttagc gtatcacacc gaattgcaaa ttcggaggag aatttttttc ctcttcatct
     4501 gggaaaaaaa gctcgtggat aaagagaatt acgaaggaaa cggaaaatat ggtgtcatgg
     4561 gccggacttc acaaaaatta tggagtttat ggcgtggatc atttaactcc aattatttga
     4621 attgtatccg aatttgcaat ccgccacccg aattcccaca tggtttgccc cgggcattct
     4681 ttgctcatac tattttttca ttgcccggag ttatggattc ctgcatcatg gcctttcgca
     4741 gtacccctgc ttatgataca catgacttta agaaagaaaa ctctctggtt tcttggagct
     4801 ttgagctttt ttgcctctct tgccgcggct ttcgccgttt tctggctgaa caggaatgat
     4861 tcttctcccg cggaatggaa tttcaaccct atctggacaa aagcggaaaa agaacaaatt
     4921 cttaccgcag cgaatttcac agcccatcga agcccggaac gtttcattta ccgcgggctt
     4981 tccctcaatt cacgccatat tgactggcac atgttttccg aagtttccat gcgccaatat
     5041 atgaaggacg aaatcaggga acggcttcag atgatacggg acacggcgga aagcgcagac
     5101 ggacgctgca tcactccatc ccatgaaacc ctgctgcatc tcgccgccgg gctcggagaa
     5161 gtgcagttgc tgaaccgcct tttacagcgc catgcggacc cgaacgtcca gatcatcctg
     5221 ccgcaacagg cctttccctc cattccggac tccgaacagg gaaatacccc cctgacttac
     5281 gcctgccagc caggcatgga cgactccacg cccctgccgg tgcataacag gctctcctgc
     5341 cttaatctcc tgattcagta cggtgcggat gtgaataaac cggggccgct gggctggccg
     5401 cccctcgtca tcacctgtgt agccggtatt ggaggagcac cctatgagga agcggccctg
     5461 tttcttctga aatgcggtgc ggatatttcc ctccagcctg aattgaaagg taaaaaaaca
     5521 agtctgctca catgggccgc ggccgccgga taccccctgc tggtccgcaa gctttgcgaa
     5581 gccgggtacg atcccaattt ccgcggggaa atggccccgc cccttctttc cctgaacctg
     5641 aacactccga acgccgcact gcaaattgca catattctgc tggaatacgg agcggacgtg
     5701 aatgcggcca tgcccgcgtc caccaccctg ccggaaaccg cgggagacac ggccctgctg
     5761 aacctgtgcc gccagctggc tttcattgat gtgtcctgcc tccctcaaat cagggatctg
     5821 gtcaatctct tcatcaaaga gggagctgac gtcaaccatc aaaacgccgc cggagaaact
     5881 cccctcatgg catgctgccg aggcatgctg ctgggagacg attccctgga ccggctgaaa
     5941 ctgggtattg cgcgtcttct gctggaccac cacgcaaacc catccctgaa agacaaatac
     6001 ggacgcacag ccctccagag gataggcaac cggtcaaacg aacatctcca gatggtgctg
     6061 aaatacctgc cggaattaag cgctccaccg cttctgaaga aggaaaacca ttgaatcccg
     6121 ccgatcctta cttcacccgg gcatttaccg tatccagcag aatgccggca tccggttggg
     6181 acccggtgct gatgaaatgg atataactgg ctccattagg ggacttatta gccattttca
     6241 acgttccggc cttctttccg tccagagaca ccacggcctg cccaccctgc caaagaaggg
     6301 aaatttcatg ccacgctccg ggcgagaaag gcactttttt catccccagc aacagatggg
     6361 gcgcaggtct cagcctgatc gggaaggtaa acagggcata atccttcgta gtggaatcac
     6421 aggcattgaa cagccggtcc gtcagagaga cctgaaggcc ggaatcatcc gcctgctccc
     6481 cgtccgctac acggaagcgg aatttgacca gccccgtggt cccgttcgga aagttccagg
     6541 tagctccgcc gttccggtaa tccacattgg atttttcatt gaccagttcg ggatcatcca
     6601 gacgcctgat ttgcaacacc ttcttcgtgc ccccggacgg atcctgaacc agctcggcgg
     6661 aaggcttgcg gttataactg caatggcctt ttttctgggg gatataagtg tgaatggtcc
     6721 actgggaatc caaatctttc cccgtctgcg tggcacgcgt cttggcccct acccagcggc
     6781 ggtccacaat aaccaggcgg cggtggtttt tatgctgccc cagggaaata aggatgcggt
     6841 ttttgtccag ctgcaccatt tcgctctggt gtttgccgcg gtcttccgga ccatccagcg
     6901 tagcatagcc gggatggttg cggtgttcgt ccaggataat ctcccggaac ccgtaccagg
     6961 ttttgccctc gtccccggac atagcaatgt gatgggaatc acggttggtg aatacatcct
     7021 cccacgttcc gttgccagct gtggcgtttt caggcagagc cattgtattc gtccacaggg
     7081 aaacgatagt tccgtcatcc aggcgtccca acgtgttcat ggtcagagtg ccgaaaaagc
     7141 gggaaggctc cggtttgctc cacgtttccc cgtaatcctt ggaaaaagcc tgccacgcct
     7201 ggtcctggga ggtgcgggca agcgcccaca acgtaccgtc tttcatttcc aaaaccgtgg
     7261 cttccacggc attgttgaac catctgacgc cctggtgggg gggcctggct tcatgtctgg
     7321 gggaagtaac accgttcctg gnnnnnnnnn ncatgccagg tcagcccccc gtcatcggaa
     7381 atatggaact tggtgccgcc gcccatgttg tggaacggca cgatcaccct gcggcccttg
     7441 ttcacaaaaa cgggggttcg catgatgccg cccagttttt tcaggttttt cctcttttcc
     7501 gggtctttcc agtcttcttc cagtttgccg tcattcgtga cggcaagcca cttgccgtcc
     7561 agcccacccc tggaaagaaa tacaaagccc ccgataggct gcacacgaat gaattccctg
     7621 gtcaaagggt tgcgcacaat agccggagat tccttgggaa tgccaccgta gttgggaggg
     7681 tagccagccg gagcggcctt catttcccat gtttttccgt tatccctgct gaccagataa
     7741 tccggttcct tgcgggtacc ggaataatgg cgtatttcac gggagtccac acggataagg
     7801 ccgcgccccg catcagccgg cggagtggcg acaacttcag gctccggaac gggtgcggcc
     7861 ttctgttggg cataacaaca acaaaaggaa ccggtcaaca atgcaaacaa caggtttttc
     7921 ataaactaaa tatcaaaggc cgtatatttt cctcaaccgg ggctttccct tcaagcaaaa
     7981 tcctaccttc catcagaaaa cgatcatcag gaatgctttc ccgcctgctt ccctccccgg
     8041 tattttgcat caagcttttt caggagtacg gccagttcct tttccaaaac ctcgtacccc
     8101 ctggcattgg gatgcaccac atccttcatg aattcattct gcggagaagc accatctgcg
     8161 gaaagaagcg cctttcccgg atcggcaaag aaaatgtcct ttccgttatg cagttccgcc
     8221 aacagtctgt tcgtctgctt caccggttcc gccaggtttt tctccttccg tgggagaacg
     8281 cccagcaaca ggattttgga agaggggcac ttccagcgca ccaggcggac aaatgccctg
     8341 atattgtccg cacatgcctg cggcgtatcc ttgcggtgcc ccagattatt ggtccccagc
     8401 aacacaatga tgacccgcgg agaaatgccg tccagctccc cgttttggac acggtaatag
     8461 gcattatcca cataatcaaa tccaaagccc atattcgtcg ctgcatgcgg accgaataat
     8521 ttctcccagg aacccgatcc cgtatttttt ccgtccccgg aaggctcccc tccccaatgg
     8581 tgggtaatgc tgtcccctat catcacgtat tcaggcttga cggcccggtt tcttttttta
     8641 acggcttcat gccgggcatt ccaatcatac gggtcccgct gatggggctc cggcttcagg
     8701 gtagcgggca acgcggaggc cggaatggaa ggcgcagaaa atgccagact ggaactcccc
     8761 agcaatatgg cgagtatgta ggagaggaaa ttcataatca aaaagtaaaa ccggttttca
     8821 gtttaagcaa atcctgccgt gcatcaagat cgcaggcgtc acttccgccg gcagagtaaa
     8881 aaagaccact gcccgtccgg caaatcctgc cgcaaccacg gccaggcgag caccgtgttc
     8941 ggcctcttcc aaggcgcgct ccatttcatc catcccccgg cctctcccgg aacaggaacg
     9001 ccctcccccg tattcatcta ccggggagcg ggcagcttgc cgtacttgtc gtccattttc
     9061 ttcagcaata cgctcaacga cttccccagt atgtcgtacc ccctggcact gggatgcagc
     9121 ccatcctcca gtagcccggg ttttggggac actccgtcct cccccaggaa ttcctcgccg
     9181 ggattggcga aaaacacgct gtttccgtca gccaggccgg atatcatttt attggtttcc
     9241 ctaatcaggg gagccagctt ttttccctcc ctggggagaa tgcccagcaa caggattttg
     9301 gaagaggggc acttccggcg cgccaggctc acaaaagcct tgagattgtc tgcgcagacc
     9361 cggggagcat ccttccgccc gccaagatta ttggttccca gcagcacgat gatgacccgc
     9421 ggagaaatgc cgtccagctc tcccagctgc aagcggtaat aggcattatc cacgtaatcg
     9481 gaaccaaacc ccatattggt taccgcatgg gaagaaaaca ggctatccca ggcggttttt
     9541 cctgttccga gagccctgtt gtccccggaa ggctcgccgc cccagcggtg ggtaatgctg
     9601 tcaccaaaga cgacatattc cggtttgacg gcccggttcc gctccatcac tgcttcatga
     9661 cgttttttcc agtcatacac ggcccgcggg ttcacctggg gcttcagcgt gggcggcaat
     9721 tcatcagcac aggccccgga gaaacaagca acgcaaaaaa ctcccgccag caataaacaa
     9781 aggcatgcat aagatttcat aacggtaagc gttgagatac tgcaatagag attaggaaag
     9841 taaagagaaa taatcttccg tcacttttcc gcaatcgcat gttcccctca ttttccgctc
     9901 cgcaacggac agacaggtga aacatgccaa agccagcgca cccgcgttct cttcgcaaag
     9961 aattagcctt gttgtccccc ggaattcacg atataattac gcggcgcgga gttttgccgt
    10021 ttccagcctt tcaggggctg aggaacgttt ttctccagtc ttcttcacta cattctattt
    10081 atgatcagca tagtaaaaga cttgaaggag tccaccgccc ttccgcagga atggcaggga
    10141 ttcaagccgg gagcatggac ggagtccatt gacgtacggg atttcatcca gcataactac
    10201 acgccctatt ccggcaatga agagtttctt tccggcccct cccagcgcac gcttcgttta
    10261 tgggacgagc tgaaagtcct gctgaaacgg gaaatagaca acggcggcgt gctggatgct
    10321 gatgaagaag tggtatcctc catcacatcc cacaagcccg gctacattga caaggaactg
    10381 gaagtggtcg taggcctcca gacggacgcc cccctgaaac gggccctgat gccctttggc
    10441 ggcttgcgca tggcccaaca ggccctggaa tcctacgggt tcaagatgtg cgagaaaacc
    10501 gcagacattt tcaaaaagat acgcaagacg cacaatgaag gcgttttcga cgcctacact
    10561 tccgatatcc gggcggcccg ttccgccggg attattaccg gccttccgga cgcctatggc
    10621 cgcggccgca tcatcgggga ctaccgccgg gtggccctgt acggcacgga taagctgatt
    10681 gccgaacgcc gcaaggacct caaaaaccgt gaacattccc cccttacgga tgagctgatc
    10741 cgtctgcgcg aagaaatgag cgaacaaata cgcgccctgg aagaactcgc ccagctgggc
    10801 gcctcctacg gctgcgacct cactcgcccc gccgcaaacg cccgcgaagc cgtgcaatgg
    10861 acgtacctgg gctacctggc cgccgtgaag gaacagaacg gagcggccat gtccctgggc
    10921 cgcgtttcca ctttcttcga catttatttc acccgcgacc tggaacagag gctcatcacg
    10981 gaagaggaag ttcaggaaat catcgaccag ttcgtgatga agctgcgcat cgtccgtttc
    11041 atccgcacgc ctgattacaa taacctgttc tccggagacc ccacctgggt gacggaatcc
    11101 atcggcggca tgggagagga cgggcgcacc ctcgtcaccc gcagttcctt ccgcatgctg
    11161 cagaccctgt acaacctggg gccggctccg gaaccgaacc tgaccgttct gtggtcccgg
    11221 aacctgccgg aagccttcaa aagcttctgc gccaaggttt ccattgaaac gtcctccgtc
    11281 caatacgaga acgacgacct gatgcgcccg cattggggcg acgactacgg catcgcatgc
    11341 tgcgtgtccg ccatgcgcat cggcaagcag atgcagttct tcggagcgcg cgccaacctc
    11401 gccaagtgcc ttctctacgc cctgaacggc ggcgtggatg aactcaaggg caagcaggtg
    11461 gccccgccct ccccccgcta cacggaggaa attctcaact atgatgaggt gatggccctg
    11521 tacgacaaga tgcaggactg gctcgccaaa acttacattg acgcgctgaa catcatccac
    11581 tacatgcacg acaaatactg ctatgaacgc attgaaatgg cgctgcatga tccggaaatt
    11641 ctgcgcacga tggctacggg aatcgccggg ctttccgtgg cggcggactc cctgtccgcc
    11701 atcaagtatg ccaccgtaaa agccatccgc aatgaagaag ggctgattgt ggacttcaag
    11761 acggaagggg aattcccctg ttacgggaac aatgacccgc gcgtggacga catagcatgc
    11821 agcctggtca gcaatttcat ggaaaaactg cgcaggctcc acacttaccg caattccctg
    11881 cccacccagt ccatcctgac gatcacctcc aacgtggtgt acggcaaaaa gacgggcaac
    11941 acgccggacg gccgccgagc cggggaaccg ttcgcccccg gagccaaccc catgcacgga
    12001 agggacagga acggagccgt ggcctccatg ctctccgtag ccaagctgtc ctatgacgac
    12061 tccctggacg gcatctccta caccttctcc atcgttcctc aggccctggg caaggaggaa
    12121 cgtgaacgcc gcgtcaagct cgtctccctg ctggacgcct actttgccgc tacaggtcac
    12181 cacattaatg tgaacgtact ggaacgggaa acgcttctcg acgccatgga tcacccggaa
    12241 aaatacccgc agcttaccat ccgcgtttcc ggctatgccg tgaatttcat caagctgacc
    12301 cgggaacagc agcaggaggt catcaaccgc actttccaca cccgttaacc cggactgcaa
    12361 gcggatgccg caggtttttc cacaaaacca tgatccggac ggagaaagct ccgtcaccgg
    12421 tttggttcat tccgtggaat cctgcggcac cgtggacggc cccggcatcc gcttcgtcct
    12481 tttcctgtcc ggctgcagcc tgcgctgccg ctactgccat aatccggata cctcctacgt
    12541 gcggaggggc cggctcagga gcgcggatga tgtcctcaag gaaatagccc gttaccggga
    12601 ttttttgcag gcggcgggcg gcggagttac cctctccggc ggggatcccc tcttccaacc
    12661 ggattttgcc ggtgccgtgc tgaaaggctg ccggaaactg ggcctgcaca cctgcctgga
    12721 cacctccggc catctgggcg tgaatgcggg cgaagaaatg ctggcggata cggacctggt
    12781 cctgctggat atcaaggcat ggaatccgaa acggtatcgc gccctgacag gcggggaact
    12841 gcgccctaca ctggagtttg cggaacgcct ggcctccctc cgcaagcccg tgtggctgag
    12901 gtatgtgctg gtacctggcg ttacggacaa tctggaagaa attgaggaac tctcccgcca
    12961 tgcggcgctt ctgggcaacg tggaacgcgt ggacgtgctc cctttccacc agctggggag
    13021 attcaaatgg gatgaattga agctggacta tactttgaag gacgtcccgg aagcgtccgc
    13081 ggaactggcg gaacaggtcc gcggcatttt ccggcggggc ggctttccgg attgcgtgca
    13141 ttaaccggat tttcgcgctt tggcgccacc aaccgcagca aaccggacgc aggcatcccc
    13201 ctcttccgga tgagagtccc tcttccctcc actacagaat ccatgcaaaa aggaagacgc
    13261 atttctggaa ttgctccgga atacggcatt tccgcaattc ctttcgttat tccatcattc
    13321 tttacggcaa cggcagcatt cccgccattt ttccgccaac gccgggggac ggcattccgt
    13381 cccttcccag attattcttt cccggaaatt tcccgcaagg cttccagtac gtcccgcacg
    13441 gcaatgtggc ggttgcaggg aacatcccgg tcacattcct tccggaagca gggagaacag
    13501 cactggtggc tgtaaaggga acggttgaat gtgcccatag ggcggtaaac atccggaagc
    13561 cgggtggaaa acagagtcac caccggaagc ccccggaaag aacagaggga cggaaagtcg
    13621 ccatccacgg ctactgccgc cgcagcgccg tccatgacgg gaaagagagc ttccggcgca
    13681 cccaccgcca gctccacatt caaatgttcc gccagggacg cggcgcgctc ccggtcttct
    13741 tccagagccg ccagcaccgt ccgtcccggc agaagggaaa ccagctccct ccactgctcc
    13801 cagctccatt cactggcgct ccccaggcgg gaaaacggag cgattaaaat gggggcatct
    13861 tccgccgggg ccgctttttt cacagggaac aaaggcgggg tggcgacgtc cagcccatgc
    13921 agccccccca attgcagata aagctgcacc cggtgcgccg ggggggcggc gcgcaggacg
    13981 ggggccttta cccggaattt gtacttccgg gcaccgggat gcgtgtccag cccggaaaac
    14041 atcatggggc catacggttc cagcgctttc agggtttcca tatctccatc cagcatgacg
    14101 cccagatcca gcggtccgtc attgtaaaat tcatctgctg ccaaagcttc ccgaagctgc
    14161 ttcagggagt cgtgcggcag cacatgggta acttccggca ccgtcttcca tatccccagc
    14221 tgggcagacg ggcagacgat gttcacctgc atgtcgcacc ggacggtttt caaagcacga
    14281 atcaggggaa cagtcgccag ggcttcatcc agcgccctgg gaacggctac cagaatgcgg
    14341 tacggcttca gctccatatt ttccaggagc gcagccgttt ttttatcctg cggggcaaat
    14401 cggtcaacgg atttccagcg atggtgcatc cagaacacat ccagcacgga ttcccggatc
    14461 aacgtttcca gccccctgtt cacttcaatc gtgtcccccg ccagagagcc gtccgacctg
    14521 gaaaaatcca ccacattccc catatccgct atccagtggc cgggagcatc cgtgcgcacc
    14581 gctatggcca ccatgtccgc gccagccctt tttctcagca gagcgggcag cggagtcgtg
    14641 ccggtcacct tgccgaaaaa ggggacatac accccctccc atacgaactg gtcgctcagc
    14701 acgcccaggg ctcccccttc cttgagcatt ttcatgggag ccttgatgcc tccctccttg
    14761 gagaacatct gagttccacg ttccgtgcgc cgctggtaca cgtattcctc catcagcgga
    14821 ttgtcaaact ggcggtacat ggacccgtaa cgctccacct ccgggtaaaa tgcgcgtatt
    14881 ctcgccagcg cctcccagtt gccggagtgg gctatggcgc aaacctgccc gcgcccttcc
    14941 agcacaggcg cggcaaactg ttcgtgccct ccgaccgtca cgcaatgctt caactgctca
    15001 tccgtcaggg tggccgtctt cgcggaacac aaaaaattgg caatggtcct tttgaaattt
    15061 tcccggctta atttctgcag ttcctttccg cgcagggccg gatccaaaac gatgcgcaga
    15121 ttcctttcca caatgttccg gcgctgggga agcagatgcc acgccacggc ccctgccgcg
    15181 cgccccagcc ggtacagcgt tttcatgccc acgagcttca acagcccttc catgagcaga
    15241 aaccctttca ttcccaaatg atgggcaaac gtattccggg aagccgggga ggaatcagga
    15301 gaggaagaat cagtcataag gaaaaccgga aaagatgtta tacgaatccc ctccggtcca
    15361 caaacccttt attcccccgg cagaggtccc ttcccgcatt ttattttccc ggctaaatga
    15421 cgtcttccct aaatttttcc gccggcgcgg aatccaaagc catcccatgc tttactgcat
    15481 cttcctcata aaagcatctc tgcggagacg gcatcttact tttggcaaag aaatgacttt
    15541 acaattctta actaaacggt atattccgtc tcccgaaccg aacaccaaac cggagttata
    15601 caccatgaaa gttctttctt ccttagcctc tatgaagcgc cgccacgcag actgccaaat
    15661 cgtgaagcgc aagggcacgc tgtatgtgat ctgcaagagc aatcccaagt tcaaggcccg
    15721 ccagggcgct acggcaggca cgcgcctttc caaaaagggc gtcaagtaat ccctggccca
    15781 gcacagattt caagaagacc gccgcccgcc gggtgccggt ctttttttgc tgaccaagcc
    15841 tctttccgta catccatgca gcttcctgcc gtccaaaccg aattcctgga tcttcccaac
    15901 tccttctccc tgagaaatgg agctacgctg gacaaggttc gcgtcgccta cgaacagtac
    15961 ggaaccctga cgccgaacaa agacaacgcc atcctgctgt tccatgctct ttccggcagc
    16021 cagcacgcct acggttacaa tccggaagtc ccgggcatcg gttccctctg gaaaccggaa
    16081 aaccacgagg gctggtggaa cagcattatc ggccccggca aaccgctgga cacaaaccgc
    16141 ttctgcatca tctgcgccaa ttacctggga ggctgctacg gcaccacagg ccccgctacc
    16201 gcctgccctg cggacggcca gccctacggc tcccgcttcc cgcatgtgga ggccgcagac
    16261 caggcacgtc tccaggccct tctgctggac agcctgggca tagaacgcgt tcatcttatg
    16321 gggccctccg tgggcggcct gatcgccctc agcttcgcgt gccagttccc ggaacgggtc
    16381 cggagcttca tctccatcgg ctccagttac cgggcttcca tcgaacaccg cctgtccctg
    16441 tttgaacaaa tcctggccat tgagcttgac ccggatttcc aaggcgggga ttactaccgg
    16501 ggaccggcgc ccaaaaaagg gctggcgttc gcccgtatca tcggccacaa atcatttatt
    16561 taccaggagg ggctggaaca gcgcgccaga aaagaggtgg gaggcaacta cggcctgctc
    16621 acgtggatga cccccacccg cagcacgcaa agctacatgc ttcaccaggg aaccaagttc
    16681 gcggagcgct ttgacgccaa cgcctatatc cgtattgcgg atatgtgggc agagttcgac
    16741 atccgcgacc acaccccgga cggaacattt caaaccgccc tggaaggctt ccgccgcgca
    16801 gggattcccg cgcttatctt ttccattgat acagactgct gtttccgccc ggcggaacag
    16861 caggatttcg cggcgcagct tgaagccgcc catattccca cggagttcca taccatcgct
    16921 tccaccaagg gacacgattc cttcctgctg gagccggagc tttatgcgga acccatccgg
    16981 cgcattctgg cggcaaggaa gccgaagggg acggcgtaag ctttttcccg ccgcaagaag
    17041 acacggcgga gcaacgctta aaaagaagtg atgccgggaa ccggaagcaa gagggaaaaa
    17101 tcaaccatgt catgaaggaa cggccaggtt ccctgcaaag cggaacttca cggctcttca
    17161 acgtgaaaat aacctgaacc ggatgaacct gaacagggtt ttcgctgtca caccttcatg
    17221 actgatcatt attacacctc cgaaacggag aacgtcggac aattccgctg cgaaactacc
    17281 tttattcctt ccaacgttac gtttgccacg gacctgcccg cttcttttga aggccgcgca
    17341 gaattcccct cccccggagt tttgctcggt tccacgctcg cctcctgcat gatgagcctg
    17401 gtttccgtgg tcgcgaccag aaaaggagtg gatttgaaag gcatgcgcgt tctggcgcgt
    17461 gcggcggaat catccaaggg aattgaaaaa attgaactga aggcaatcat gcccctggaa
    17521 ggcagccacc ctctccgcgc catgctggaa aaaaccgcct tgtcctgccc tgtgcgccag
    17581 gcgctgcggc ccgatctgga aacgcccatt gaatgggaat ggaagtaagc agccatcagg
    17641 gacgtcaacc caagcttgaa gttatcctta aagaaatccc gcccattcat gaaaaaagcc
    17701 gctgcggaat catccgcagc ggctttccaa ttggccgacg ttttttcctt tagaagttgt
    17761 agcggtagcc gatactcccg ttgatggagc tggaaccgct gcggatatcc gcattgccgt
    17821 ccacaaaaat gacgccctgg gtgcccacgg gaacgctcag ccccgcgcca atctggaagg
    17881 ccgtgcttcc gaccttggcg ccatacaccg gccgcgtata ggagggatct gccaggaagc
    17941 ccacattagc ctgcccgcgg cggtctccca tatcctggga aacgttcacc cgtacttctc
    18001 ccagcgcttc acgcccgaag atgttggacc ccatgagtcc ggagagccgg gcacccacag
    18061 cgacagcagc cgtagtcagt tcctggtctt ccaccttcag tccggcattt ccggcagaac
    18121 cgctttcccg atagccgtcc atgcgggtcg tcacgatgga agcgttaaac aaaggctgga
    18181 gaatcacgct cctgtcttca ttgagagcga tgtcatacgc caactcgtac atggcgccga
    18241 aaccggctcc cgtcgtattg ccgcgggcct ggtaagaccc cgtaccgtaa ttcaccgtac
    18301 gcgtcagcga ggcatcattc cagccacccg tcaaaatcaa cagatgggac catttacggt
    18361 actggtagcg ggcgaacaga ttgacatagt aactgtccag gtctccgtcc gccgtatccg
    18421 ccgcgctggc cgtcaggctt ccgtaattgg cagtaaacgc agctcccgca gtgaggcggt
    18481 cgttgatatc cacgtccatg cctaccgtgc ctccccaggt ggtgagttcg tagccgcttt
    18541 catcccccct ggtgtccagc ttggcataag acccggttcc ctgcatccac atatggaagt
    18601 agggcatgtc ttcatggaca tatgcagggt taacgcccat ctgcgccacg cggttgcgta
    18661 tccagcccat ctggtcgcgc agggagtcgc gctgggcgat gcccaggctg gtaaccgtgc
    18721 tgccagcggc ggcggccagt ttgcgggcgg cgccggaata gtcgcctgaa ttctgggaaa
    18781 gaagaacaga ggtatagaaa tcgcgcaagg cagagccttc ctccatgtga tagcgggaat
    18841 tccaaaggat gtccgctcca gtagcggcat tccgcgtcag ggaagagtca cggaaaatgt
    18901 tttccgtgcg cgccgtaccg gtgaggatga tggccgtcat gctgtcattg gtggtcagct
    18961 ccaggttttc atacatggaa ccaagaatca tgtcctggag ggtgacattg tcacccaggg
    19021 acaccagcgc gccgtccgtt gtcttgaaca attccatatt cagcgtttcc gaactcccgg
    19081 aaaccagggt atccagattg ttcatgacca gcgtgacttc ccccgtcccg tctcccagcg
    19141 taatatttcc gctggaggtg acaaagggat tgcccaggcc gtccaccgtt gtggaggaag
    19201 tggtaatggc cactgtgctg tcccctgtca cgctcaagcc gtccgagcca agcgtcagct
    19261 gggttttggg atcgtctgcg ctgccgatat tgagcgtgcc gccgttcacg gtaatcccct
    19321 gataggaagc ttgggcggga ttgccgccct cccctgccgc tccggccagg gtcagcacgc
    19381 cgccgtcact cacattcagg tgataacctg cattgcccgc acccttgata gtctggacgc
    19441 ttccgttgcc ggatttgacg ttgagcgttc cggatgcccc gtccagagta ccggtaaagg
    19501 tatgggaatc ggaagagctg atcgtcattt ccgcaccgct cagcttcaga gctccgccgc
    19561 cctccagtcc ggaaacggta ttcacggaac cggttgcagc ggacagggcg gacccttcac
    19621 ggatatccag caggacaccg ttcagggaag agccgttgcc cagtgtcagg gaggagtctg
    19681 ttcccgtacc acccaaggtc agcgtaccgt ccccggagat agtcacttcc gtgacatggc
    19741 tgccggcctg ctggccggta agaaccatct gggctccgtt acccaaattc agggttcccc
    19801 ctcccgcgcc ggcagccagc gtctccaccg tggttacacg ggaggcgccg tcattccgtc
    19861 cgcccagcgt tatgtctccg cccgcgagtt ccagcgtagt aaggctattg cctgcaccgt
    19921 tcaggatgat atttccctcc gaggaactga gactggagtc attcccggtg aagtttcctt
    19981 ctacggtgag ggtttccgca cctttcttga caaaatccac atggtccccg ctgatggttc
    20041 cgccgtagct gttgccgccg gcgtcaacat cctgaaccag atccaccacg gcgtggttgg
    20101 aagccgcatc atccgccgta ttcgtcacgg tcagcgcacc gtcgctgcca accaggttgc
    20161 tgactttcag accgtcccca ttccgggagg cttccggagc cccgttcagg ctcacatgaa
    20221 gcgtggtgcc attaatgccg acagccttgt agggatccag cgcggcgtaa tcgctgatat
    20281 tggggttgtc tgaattgggg gtggtgtcca ccagatagac aagatccgta ttgccgctga
    20341 ccatcagcgt cccgtccgca cccaggactt ccgtcacggt atagccgagg gaagccagga
    20401 gaggagagaa tccgatttgc ttgaaattat ccacagccag ggttcccgtt gtcagtacaa
    20461 gccctacgcc gtcagtggaa tcccggttgg cggcaagcag gtccagaaca ccctggttgc
    20521 tgaggttgat ggttgttgtg gcggctgaaa gcgtcaggct gtccgcatca ataacgccct
    20581 gtccggtagt tccgcccgct gtaaggctgc cggaatccag tttcagcgtc accgtcttgc
    20641 ccgtcatgtc cagagcaccg gaaacgcctg taagccggga gtaatcctcc atgacgatgg
    20701 agccgaccat ggagccggac aggccgccca gattgatggt attcagggct ccctgcgttc
    20761 cggcggaagc ggccacttcc acgtttccgg cggttttggc ttccgcgttt gccaggcttc
    20821 ccttcgtaat cagaataacg ttggccgcat ccagagagga caaattgaga acgccgttat
    20881 tgacggtgac ccgtccggac cccagggagt cggcccccgc caaggtgacg gtgcccgttc
    20941 cggagagcgt cgtggaaccg gtgaacccgg tgttgccggc cgccagcgtt atctctccgg
    21001 cagtgtcaat aaccagggaa ccggctccgg agagggaccc ggcccaagca cttccgtcac
    21061 ccagccgcgc cgtagcgcct cccgcaatgg agagtgcgcc caggaaggtg ttcgctccgg
    21121 tgagttccaa cgttccggcg tttacggata ccgtgctgcc ggccgtaccg ctgatgacgc
    21181 ctccgtagga gaaatcgcgg gcaaaggcca gcgtggaatt gttctggatg tcaatgctgt
    21241 cccccagctt ggccacatcg gcggaattca cggtaaccgt gccgcggtgc accttgagct
    21301 gaccgtccag gttgacgccg cccgccaggg taatattgcc cgctccctgg gtggagaacg
    21361 ccgttccgct caaatcgccg ctgaaggtga aatccttggt ggcaaagacc tggttgcccg
    21421 taaagacaac gtcattggca aacgttcctc cggctgttcc gttccattcc gtacggacgt
    21481 tggaatccgc cagtttcaag atgccgtccg cccccatcgt tacgttattc aattggaaat
    21541 agccggatgt cacggacagg atcccctgga attccgtact catattcagg acattggccc
    21601 catagctggt tcccagtccc acggaaacgg taccggaacc ggaggcattc aggttcagag
    21661 ccgagttatc attagccccg ctgaccgtca gtacggaacc gtccgccaat tccacggctt
    21721 ttccattggc cagagccttg gcgacggtgg cggccacttc ctcgcccagc gtcatggaac
    21781 cgcccgtcca cgtcatgttg tctgcggtaa acgccgtggc aaaagagccg gaagccaaat
    21841 tgaggcggcc gccctgcagg ttgatcatgc ccattcccgg ttgaacgtcc gccgtagcca
    21901 gagtgagcac gccgtccgta acggtaatgg ctccggatcc cagggaattg gcgacctggg
    21961 ctaccagttc gccctgctgg acggaaatat ccccgttcca gcccgtgttg tccaaattaa
    22021 ggaccagctt cccttcgccg cgcttggtca gcgttccctg ggcgattttt ccggtgccgt
    22081 cggaaacgaa gacataatcg ctgccggcag cattgttgac tgtgatggaa gcaggattca
    22141 ccgctccgga cagagtgacg gttcccgccc cggtactgtt gaagatcaga ttcttcccgg
    22201 cagccgtatc cgtggaacct tcaatgcccc acatggcgcc cgtgttcgta tcgctccagg
    22261 tcactccttc cgctccctgc cagatccata cgtccccggc ggaagtcact accagcttga
    22321 gggatgttcc cgtctgctcc agttcataaa tcagcgtttc gtccccggta tatccggccc
    22381 agttaaagac ggcgctggcg gcatcaagac cgctggcggc ggaaatcagg gtatattccc
    22441 cggcatccgt aatgccttca agcccgttca gcgtaagatt gaacgttccg gccccgaaag
    22501 cgccgcccac ttcgagggcg gagctgatat ccagtgtcag cgtagagttg gccgccaggt
    22561 tcagagcgcc cgcagtcgtc atccgcgtat tggccgccat cgtgatattc cccagggacg
    22621 tagctccgga agcagtaaga gcgagcgttc ccccctgaat atccgtcagc cccgtgtagc
    22681 tgaccgttcc tcccagcgtc agcgtttcct cgccgacctt ggtcagagac aggacgccgg
    22741 attcgtcata cgaaatggaa gtggctccgt acccaagagc aacaccacgg tctgtgagcg
    22801 tgccgttgta cgtgccggcc cccaccgtca gcgtgcgcgc accgcctacg gctcccacct
    22861 gggaattttc cgttccgtcc agagaggcaa tttcaacatc ccccagcacg cgcatgaaag
    22921 cgaatccggt cgtgtccgtt ccggatgtca gggtgacgcc accctgagag atggaagtgc
    22981 gcagatccag cccggtgcta tagccgttgg aagcattcat ggtgatcatt ccggtaaatc
    23041 cggaagcgtc tccgctgatg atgagctttc cgttggaatt gtacctgccg tcgcccgctc
    23101 cgcggctgta agtcagagaa ccggctccag tgagcgttcc ggtgaagccc atttccttgg
    23161 cccaattgtc cctgcccacc agggagaccg ccccgttgac ggtaacggtt ccggtgaagc
    23221 tatgctgata gtccgcagcg cttgcctggg aggcgtccac ccatttcagg gaaacggcgt
    23281 cggacgcgtt tgttcccaaa atcaaatcgg agttgatggt atggtgggag gttcccgaac
    23341 tgctgccggc aataatcagc gacgtgccgt tttccagggt gacggaagaa cccgcgcttc
    23401 cccagcggat ggatgaggtt ccgttcccta tctggaggtt gccctcctgc accacgatgt
    23461 ggccggagtt gctccccgtc agtttcagca agccagtgcc ggttttccgg acagttcctt
    23521 ccccagtaat ggcattgccg aaaacgatgt cggacggagt cgccagagtg atgaaagagt
    23581 tggcatccgg agaaagggag aataccgggt tagtgacggt tcccaaatcc accgtcaccg
    23641 tcgctccatt ggccgcaccc aaaaaggaac cggcctccca ggttaccccc gtgccgggaa
    23701 gagcagtatc ataaccgaat gccagaacac cctgcgacac agccacagta gtacccgtgc
    23761 cgagtatgga agaggtgccg aggaccaacg ttcccgtgcc ttccttggtg acggccgtat
    23821 tggtaatttc tcccgtcccc gtcagttcat aacgggtagt ggagttgcgg acaagcatgg
    23881 agcttggcga aacctcagaa tccagctgta cgacagagtt agcgactccg gcctggtccg
    23941 caaaggaaac cgccatacca tccgcatatg tggcgggagc gccgccgtcc gtccagacat
    24001 gttccgcgct ccagacgccg ttttcacccc cctcccacaa aaggagggtt cccgccgtat
    24061 agacaaggtc tttgccgttg tcggaaatgg tgatggaccc agccacaccg tcagcaaagg
    24121 aagccttaat ggaagatata ttggacaggc ccgtagttga aaggacggac atgccgtctg
    24181 tcagggtgcc gaattcggaa agatccaggt tcagttcccc ttcagcggaa agagagaacg
    24241 agccgtccag attgattccc gttcctgccc ctcccgccag cgccagcacg ctggaaccgg
    24301 tcatgttcag attccccgta aagaaagaat cctggaacat ggtcacgatg tcgaaaccgg
    24361 ccacagccgt attggagaag gaaatggcgt gatccgccgt attgccgtaa cctcccagca
    24421 gcaaggaaga ggtgccgcct atcgtgcctc ccgacgcatt cagcgagctg cccgtccgga
    24481 atactgcttt cttaacggta agggtagcgt ccccgtttac ggaagaccct gttcctccgc
    24541 cacccgcgta aagagcaccc gtgtacgcac cgccatccag cgtcaggctg gaattgccgc
    24601 tgacggtgga tgttcctgat gagctgaacc cgccgccgta aatagccccg gtaaagacgg
    24661 cgtcactggc ggcggtaatg ctgacgctgg aatcgcctcc aaccgtatat gccccggaac
    24721 ctcccgaata ggaaccgccc acgatgctct tgacaaaact gccggaggcc gttcccaaat
    24781 caatcgtcac attggtgctt ccggtaatgg cggcacggga cgaggaattg gcttcataag
    24841 tgctgccgcc aatgatgaag ccccggacaa cggagttgag cgaaatggtt tcgtccgtga
    24901 cgctttgcat gctgcgcacg acgacgtacg tgctgccgtc aaggttgttt acggaattat
    24961 gggctgccgt gccgccgcct atcaaactgc ccttgatgac ggcattcccg tccacggata
    25021 cgtaaacgtt tcccgtgatg cctgccgcct ggccgtcctt gtagttgcct cccacaaccc
    25081 aattgacggc agtgtccccc tgaatctggg tatggacgtc tcccgtaacg ttccacttgc
    25141 cggagccgct ccagttattg gcatagctgc cgccgataat cagattgtaa ttgcccccca
    25201 gcacatccgt ccagatgccg gtggtgattt ccgtagccgt ggcactggtg gcagtattgt
    25261 agtagccgcc ggccagggtc actcctgtca ggtctcccgt tccggtgacg gcattcacgg
    25321 aaccgaaccc ctcctgcatg gaagaagccc ggatgccgta cagggacaaa gactgttggt
    25381 ttaccagcgt acctgttgcg gaagagggag cttcttccag cccccaggca gggtcccaga
    25441 cggtctcatg ggcgccggaa gccgtaaacg tgatgactgt ttttccgtcc acggtgctga
    25501 ttgtgtacag ggactcgtca acatccgcca gagtaaagcc gttccccaaa tcaacgccca
    25561 ggttatagga ggtgttcacc agggcttcag aaccgaacaa gtcctggaat tcctgaaaac
    25621 cggagaaatc aatgaggccg gaagtcagcg tcacgttctg cagcacgctg ccgacattgt
    25681 ccatggaagt ataatacaaa cggcctccat ccaccgtcac attgcccgtg atcgtcccgg
    25741 aaccgttggc gaggatgccc atcgtttccc ctaccgtgaa ggcgttctgg aaattcatgg
    25801 tgcccgcaga ggcggagatg gtgccgtttc ccgacagtcc cgtcacggtc atggttccgg
    25861 cgcccgcatc cagccggcct ccggcaagcg cggcgttaat ggaaccggcc ctggagagga
    25921 cggcgccgga attgacggta taggttcccg taagcgccac gttctcggaa agatgcaccc
    25981 agcccgactg ggcgacgaag ttgcctgccc cggtgatgga attgtcaatg acatccgcca
    26041 catcccccgc agtgccgtaa taggtcaggt actgggagtt ggccgaagta gtaacggcgg
    26101 cagtcccaag ggagccggaa gcaccgatgc ccagagaggc aaagcttcgc gtaatggcga
    26161 tcgtcccccc aatttcattg gcggtgttga gcaggttgta ataggtatat tcattcgcgc
    26221 tgccgctgat ggaaaaaccg cttgccgccg tgagaacacc ggaaaatttt acgttcttgc
    26281 cccagctgga tgtcaggttg acgccgctgc cgagagtcac cgctccggaa aaattaaaac
    26341 cgccgctatt gatggaagcg gcattgtagg aggcagctcc cgtcacggga ggtccgtcct
    26401 gaatattcag tgtggtaccg tcacccatga ccagtttttg ggaccaggtg acatttccgg
    26461 agaacaggga aagagtgcct ctgctgattt tcagggttgc gttcggagat gccgcgggag
    26521 agagcgccgt attcttgtcg gaattgccca gggtgaaggt gcctcccccc gtatagtcca
    26581 ccgttacggt gccgccgaaa ctgccgccgt aagaggaaga atcccccgtg atggtcagcg
    26641 tggtcccctc cccggtgatc aaagccgcgg cattgttgtg ctccagcttg ctgatggaag
    26701 tgttcacgcc ggctttcagg acggaagcac ccgtcgtata accgtaaccg cccagtttga
    26761 tcgtggcgta ttgggcggcc tgcgcatggt tgaggttcag gatgttgttc tgagatcccc
    26821 ccgtcgtgtt ggaataaagg gaaatgattc cgttgaaagt gcctgccccg ttcaatgtca
    26881 gggtggaaag gctggcgccc ccgttggcca cccggtttac cgtcaggatg gcttccggag
    26941 ccgtcccgtt caaattggtg attgaggccg taccggctcc ggcaaaattc aaggtagtag
    27001 aagccgtaac agtaccgaca tcataggagg ccgagcctcc cgccgccaca ttcacctggg
    27061 ttccgtcaaa ggcgacatcc gccgcataag actgtgatgc tgtcaccaaa agaccagaaa
    27121 ctcctgccag cgtggcggtg ccaagcgtta ctccggaaaa ggaggtcatc aaggcagcca
    27181 ataaattcaa agggaggtgt aatttcatga aacgtttgat aattaggtat ttaatgaaca
    27241 aataagaa
//
LOCUS       DAWEMX010000015        27244 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_729_length_27244_cov_12.218066, whole genome shotgun sequence.
ACCESSION   DAWEMX010000015 DAWEMX010000000
VERSION     DAWEMX010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 27244)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27244)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27244
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_729_length_27244_cov_12.218066"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5507 a         6663 c         8490 g         6584 t
ORIGIN      
        1 ggaggggtcc ccccaggcca ggaaaacggc cggcggagac attccttcag caatttgtcc
       61 ggattccacc agtgttttct ggctgggcgg gagggaattg aaaagggccg ggtttttctg
      121 gatgcgcgat gccggggtgg acacgcagga agcaaggctc agagcggcgg acagggtagt
      181 gaaaagtaac ttccaactca tgatgtacat acggataaca ctcactttga ctcttgccaa
      241 gggtcttttg ttgagtaaaa tatacgaaca cgttatgaaa gcgctccgta atctggcttt
      301 aacactgata gtccctgttc tggtttcctc cctgtccgca gcggaaaagc cggaaatagt
      361 tgacgaatat ctcccggtcg gcaaaatggc ggaagccgcc gccgtcagcg ttgtgcttga
      421 tgaaagcctg cagccgttca tggaaaagat agacggcgtt ttcgcctccc tgcccgacaa
      481 ggacaagaag gagcttgtca gccagattgt ccccggccag cccgttcctt atgatgagcg
      541 cctgggctgg accaaggacg aatatgccaa gtacctggag tgctggaagc tgaagcaggt
      601 gcaggaagtg gcccctgtgg ccctgggtat tttcgcctcg ggggaacgga atatctggga
      661 tttggctgct gtggcgcagc aggggccgtt gccgatgagc acgctgaaat atgattccag
      721 taccaaatcc tggatttctc ccaacggaac cctgactctg aaaggggacg tgtcctatga
      781 cgatctgaat gtatacggcg cgtggagcgg caaggaatgg acaatggaaa agaaaaccat
      841 tctttccacc ctgacggaga ctatcattgc gggcaagacc aaggacggca agtatgccta
      901 tttcgtttac aacatgtctg aaaagaaccc ggacaacgtt gccattgcca accagtccat
      961 cgttctgcgt gttcccgtta cccgcatcgc gggtgaccct ttgctggaaa aggctaaggc
     1021 caaggctcgc cagtagggat atgatgttcc tttcatttcc ggtaagacaa caatgcgtat
     1081 catcagcgga aaggccggcg gcataaccct gtctgttccc aagggggaag ttcgtcccac
     1141 gacagaccgg gtcagggaag ccgttttttc catcctgaac ccgctgatgg accgggccga
     1201 cgtgctggat ttatttgccg ggtccggcgc cttcggcctg gaggccctca gccgtggggc
     1261 gagagacgcc cgcatggtgg atttttcccg tctctcctgc gcggcggcca gggccaatct
     1321 cgtcaagacg ggcttggaag gcggaactgt catccaggga gatgccgtcc agttcgtgag
     1381 aagggaactg ctggccggca ggaagtacga tattattttt gcagatcctc cttattgcaa
     1441 ggggccggcg gaccgcgact ttattgttga actggcggag gccggagttg ccggactttt
     1501 gaagggcggc ggcgtgttta ttgcggaagt gcaggagggc tggggtaccg gaagggaagg
     1561 cgccgcggaa attgacggcc tgaatctggt ggatacccgc cggtacggga aaaatatgct
     1621 gctgttctac caattgcctg agaagtaaga tgaaagcctt cctgttttcc ttctgttaca
     1681 atatcctgta taccgtcggg tggctggtaa cgctgccttc ctatctgctt aagcagaaga
     1741 ggagaggcgg attcggcacg gggcttctgg agcgtttcgg gctgtaccgg gtctcctata
     1801 accgggagcc gaaaggggtg ttgtatgtgc atgccgtgag cgtgggtgaa gtggtgctgg
     1861 ctctcaagtt tctccgggcg tggctccggg aacgcggagg ttccgccgtg ctggctacca
     1921 gtacggctac ggggcatgct acggcagtga gtgcgcagat tcccggcgtg cgcgtgattt
     1981 atgccccctt tgaccttctg gggctgccgg ggaggtgttt tgaccgtttt gaaccggagg
     2041 ccattgtcct ggtggaggcc gagttgtggc cgaattttgc ccgtgcagcc aaggtgcgcg
     2101 gcatacccat ggcgatgatt aatgcccgta tgtccgccag gtccgagagc cgctaccgcg
     2161 catttaaatg gatttcaaag tattattttt ccttccttga cgcaatgggc gtacaggaca
     2221 agggggatgt ccggaggttt gaatccgtgg gcgtgcgttc ctccattatc cacgtgacgg
     2281 gaagcatcaa atttgaccag caaatggcgg agcgcaggga gactaatgcg gagtttgcct
     2341 ccattttgga caaactgaaa cgcggcaagc ctgtagtgct ggccgccagc acgcatgacg
     2401 gggaggaagt tctgatcgca gaagcggcgc gcaaggcggg gggcttcccc ctgatcgtgc
     2461 ccaggcatgc ggaacgccgc catgccgtgg tgcgggaact ggaggcccag ggctggcagt
     2521 gcgtgctgcg gactgatggc gagattccgg aaaccctgaa ggatcatgtt tgttacatcg
     2581 cggatacgac gggggaattg agggactgga ccgcgctggc ggatgtagcc gtgatcggaa
     2641 aaagcttttt ggcggacggt ggacagaatc cggctgaagc cgtcgcctgt ggcgtgcccg
     2701 tgctgacggg gccccatatg gaaaatttcg atgcactggt ccagctgctt gaaggagtgg
     2761 acggaattgc caggtgtgat gaaaaccgcc tggcagacgt gctcaaggag atgctggaca
     2821 accccctgct ggctcatgcc cagtcttccc gcgcccaggt ggcgttgaag gcccatttcg
     2881 gcgctacggc ccggaccatt cgcatgatct gcatcatgct caagattcct gtttgatatc
     2941 ggtttttttt tgcccctggg gctccggcct ggaatgggga ggaagggcga agacttcccc
     3001 ctgcggagca tcaatgatga tgtcaaaatg ctggaaagta tccacgccga tctggtatcg
     3061 gccccctgtt cccgttacgg cgaaggaaag gccgtccatt tggaagtccg gtcccatgcg
     3121 gagcgtggag gggataccca gtttcatgac agtgcagttc ccattgtggc cgttaatgtc
     3181 ggtcgccttg gtggtgagct gattgccgtc cggatctgcg ggccactgtt ccagcggggc
     3241 catggaaaga gaagagccgg agtcaagagc cagagggaaa gtgtctcccc cccgggaaca
     3301 gcggatgtaa aaaattccgg aaggatcacg ttccacatcc agcggtttca tgtgttgggg
     3361 gaagcgggag cgttccagaa actgcaggga agcatttctg acggacagca ggaacggaga
     3421 gaaccccagg tagttgatgc ccaggatgcc gtctatcttc acctgcatgt ccttttgcag
     3481 gggagtaaga tccagggcaa ggccgaaaaa gtttttaata tgaagcccgc ccatggagaa
     3541 ggaccgcatg gcaaatagcc gggggacggc atcgacgttg gaaccggggg cgatttgaac
     3601 gtcttcaagc ggcaggccgg gaaaattctt tttgatgaat ttgatgtcaa atgtggtgtg
     3661 ggaagccgcc gtgtccacaa taagattgca tgggacccca ttgacagtgc attgcgctac
     3721 aagaagcttg ctgtgggcat ccctctccag ctttacgggc ttcaggtcag taatggacac
     3781 gacgggaccg ccttgagcag gaaccggctg ttcttctgca gataagggaa gcgcccccaa
     3841 tgccagaaga gccaggaaag ttcggatgag gctgcgtgaa aatagcattc ttcaccatta
     3901 ggtgtgtttt tgcagtttga caagccaatt ttgacggcga atcagtcatg gctgcggtgg
     3961 ttgggcttgt ggcggcgttc cagttccacg cggtcttcat agttccgggc ctgcactttc
     4021 cggtctcttt tcccggcccg ttccacgttc atttgccttt ttttctgacg tttggcaagc
     4081 cgggcgattt cattttccaa attcaggaag tttaagtatc gctccttgtc cagcttcccc
     4141 tcgtccacgg ctttcctgat ggcgcagccg gaatctttac cgtggctgca atccgcaaat
     4201 ttgcattcat gggccagctc cgtcaaatcc gcaaagcttt cacggagggt atgttcatca
     4261 gtccacatct ggatttcccg tatgccggga ttgtcaatca gcaaacctcc atgcttgagg
     4321 agcaccagct cccgcgccac tgtcgtatgg cgccctttgc cggttacttc attgacttct
     4381 cccgtccaga gccattcatc ccccagcagg gtattgacca gggtggattt cccaacgcct
     4441 gaggagccga cgatgcagat ggtttttccc ttggataggc atttgcggaa ttccgtgatt
     4501 cccctgttgt gcagggcgct gatgctgatt atggcgcatt ccggggagag ctcccgcaga
     4561 atctgcatgg cgttttccac ttcctccctg gaaaaaagat ccgttttgtt aagcaggatg
     4621 agaggagatg ctccgctgcg gcggatgagt gtaaagtagc gctccatgcg cctggggttg
     4681 aaatccgttc ccggttccgt cactacggcc acgatatcca cattggttcc caatacctgt
     4741 tccgcgctgc ttttccctgg agctttgcgg gaaaaacagg tctggcgcgg gagaatggag
     4801 cgtatgacgg cttcgtctcc cgcttctccc ggatcaatcg ccacccagtc tccaacagcg
     4861 ggcagttccg catccgtggc cgcatcatgc cagagtttgc cgctgacgac ggcgtccaca
     4921 tcttccccgc cgtccagaag agcagtgaag ttgattttcg tttcccggat gagacgcccc
     4981 ggcatccagc ctggtttggc aacggcgtcg aaagcgcgtt gaaaatggtc gttccagcct
     5041 aaatcctgaa gagtgacggg catgaaaagt gaaaatgtgg ttctgggaaa ggtggccggc
     5101 atttacatgc cggcattgga tcgtggggca aactccctgc cgcttcaagc gtggaacggg
     5161 tctgccgtgc gccatgacgg agcttttccc tgtgcgagac ggcaagcgcg ctcaatgatt
     5221 tgaagggcaa tttgtgcttt aggcgctttt tccaggaact ccgtccgggc cgggtaaacg
     5281 agcagcactt cattttcaga ggagttaaaa ccaatgtcac tgcgggaaac gtcgttggcc
     5341 acaatcatat cgcaccgctt gcgttccagt ttgccgcgcg cgtaattttc cacgtcatgg
     5401 gtttccgccg caaagcctac cagaatgccg ctgaagccga attccgcacg catggagccc
     5461 aaaatgtccg cagtacgttc cagctccaaa atcatgcgtt cccccgtttt cttgatttta
     5521 cggtccggaa cggagacggg gcgataatcc gctacggcgg cgctgaggat ggcggcatcg
     5581 gcgtaaggag cctggttttt gacagcatca tacatttcct gtgcacattc aaccggcagg
     5641 aagtccacgc cgtgcggaat gtccagggaa gtggggccgg agatgagaag gacgctgtgg
     5701 ccttcgtgaa cagccgcttc cgccaggcaa taccccattt tcccggagga gtggttggtg
     5761 aggtatctga cggggtcaat agcttccctg gtggggcctg ccgtgataag aaaacgcatg
     5821 agaggcatta tgcggaatca cgggtaaaag tgaagagtga tcttgtgcag ctgcgcatta
     5881 tgttccggct tgactccttc ccgcgattgg gggagttatg aaggcgtgat ttattgggat
     5941 aataacgcca ccacaccgct ggctccggag gtttacgagg ccatggagcc gtttttaaag
     6001 gaacggtttt tcaacccgtc cgccggttac ggctgtgcca ggcatgtgcg tgaggcggtg
     6061 gatgaggccc gtgcggccgt tgccgctctg ttgggcgcat ttccttcgga aattgtattt
     6121 acttccggcg gaacggaagc gtccaatatg gctttccgcc agatggccgg gagacaggag
     6181 ggggggatag gcgttttgtc cacggaccat gacgcctcct tgaagacggc cctggccctg
     6241 ggctgcggct gtgtctgccc cgtggacagg gaagggagag cgattccgga aaaatgggag
     6301 tcgatgtgcg cagggggcgt atccggcgtt tcttttgcct gggccaataa tgagacaggc
     6361 gcattgcagg atgcgccggt tttgtgttcc tccgccagga agcatggagt tcccgtgcat
     6421 ttggatatag tgcagtgcgc cggaaaaatt cccgtggatt tgcatggaat ggaagtagat
     6481 tatgcttccg tatccgcaca caaattccat ggccccaagg ggattggatg tctgtacagg
     6541 agagcggggg ctcctttttc ccctttgctg ttcggcgggg ggcaggagta cggcttgagg
     6601 tccggcacgg agaatgttcc gggaatcatt ggttttggtg cggcggcgcg ggcggcattg
     6661 aggcacttgg aggaggacgc cggacggctg tcccggatgc gggattcttt tgagtctctc
     6721 ctgcggacac aggtgggcgg cgtgacggtg cattccggcg gaacggaacg tattcccaat
     6781 acttctaatc tggcgtttga cggatgtacg gcggaagcgc tgatgctgtt gctggaacct
     6841 gctggtctgc tgtgttcggc agggtctgcc tgccatacgc tgcagccgat gccttcccac
     6901 gtgctgacgg ccatgggatt atctgatgag gcggtacgtt cctccctgcg tttttccctg
     6961 tctttcatga cgacgcagga agaggtggag caggctgcag ccctggtggg ctccgctgtt
     7021 cggaaggtgc gctgcgtcca gtcttcgcgt accggcccgg tgcttgtgta caggccgtag
     7081 ttgaagggcg gaaaggctgg tagctctttc agatggcctt ttgtgttcca tgatccgggg
     7141 gacagtgatg tggtgagggg gaaagggtgg aaaaaagctg tccgtcatgg cctttccttt
     7201 catttttacc ggaaggcggg actttctttc ttgagttcca tgcctcctcc atgctagctt
     7261 gtataaacta caggaagggt tgctgcattc ctgttattga tgtatgaaga tagaacatta
     7321 ccagaatagg gatgaaacgg aggttttgaa tatttggctg aaagcgtccg aacaagccca
     7381 ctcttttgcg ggagtggggt tctggtgcgg tttggtggaa gatatgaggc aggtgtacct
     7441 gcccagggcg cgtacatggg tgtgcaggga cggtggccgt gttgtcgggt ttgcctgcct
     7501 ggtgggggag ggggaactgg ccgctctgtt tgtggcgccg gaacgccagt gtgaaggaat
     7561 aggtacagcg ttgctggact gggtgaagaa acacagcaag ggaatgctga cggtgggggt
     7621 ctatgaagag aatgcccgcg cccgcgcatt ctatcggcgc ggagggtttg aagaaatcgg
     7681 ctccagggaa gatgagctga cgggaagccg ggagtaccgg atggaatgga aaagagagag
     7741 gatatgatcg ttataaaaaa ggcggaagtt ccgatgaact tccgccccct gtaaagcagg
     7801 ccggcgttag gcgagttcca ccttgacgcc gctcttttca atctggtcgg ccatttccgg
     7861 agaaagggcc tcatcagtga tgagcatgga aacctgggag ggcgcaacgg tatagaatgc
     7921 cgggttgttc gctacgctgg tggagggcat ggcgatgatt accttggcat ccgcggggat
     7981 gacgctcttg atgaattcgg attgcaacat gttcttgtat ccggcgccat gcttggggga
     8041 gtagcagtct ggcgtcagca cgaccatgtc aatgctcagg gatccggcgg ccttggcggc
     8101 gtcctgccct acatagacca gggcttcgcg gtcgaagtgt ccgcccgtgc aatagaagct
     8161 caggttcgga atgccttcaa gccgggtcag cagcgtgggg ttgttggaaa ccacatgcgt
     8221 atctttggaa ttgatgtggt tgcatatctg gtagcccatg gtgctgctgt caatgaacat
     8281 aaccacggaa gcgggaatat tctgaacggt tttttttgca atcctgatag acacatcatc
     8341 ttgagtgaca atgtcttcat ggagggattt gtgagtaagc gccagtgcgc cgccgtggac
     8401 gcgcttgagg aagccgcgtt tttcaaggtt aatcaaatca ttgcggaccg tttcatccgt
     8461 gaccttcatc tgtttggcca gagcaatggt ccttgcagct ccatgttcag ccagcgtttt
     8521 caaaatgaac tctttgcgtt gggatgctaa atacatttga ttttgttttt ctatgtagaa
     8581 tgtttacacg ttctaggtgt taagttgttt tctaacaatt tttcactaga acagtcaagc
     8641 gttagaaatt ctaagttttg attgttattg tgaataatgc agattttgta ataatgggac
     8701 aactgaatac cgagaaatgt acaaaaataa ttttacgatg cacaaattag ttatcgttat
     8761 gacagatttt gtcaatctgg aatgatgatg gaggcaggat caaagttggg agtggggagc
     8821 gaaagctgga gatggcgcag tttttctacc atcaggcggg cgacaaccag atttctgtac
     8881 catttccggt ctgccgggat gatgtaccag ggagcttccg gagtagacgt tttttcaatg
     8941 agatcctggt aggctgtctg gaattcatcc caacgattcc ggtcatccag gtcatccatg
     9001 cagaatttcc aaagtttatc cgggtcctga agtctggatt ccagccgttt tttctgttcc
     9061 gccttggaga tattcaggaa tagcttgatg atgacggtgc cttcttccgc aagcatggat
     9121 tcaaagtcga ggacgtgctt gtagcggcgt ttccagactg ggtccgggaa gatttttttc
     9181 acgcggacgg caatgatatc ttcgtaatgg gaacggttga agatggcgat ctgtcctttg
     9241 gcggggacct ccttgtgaac gcgccacagg aaatcgtggt ctagttcctc agtagtgggt
     9301 tttttgaagg ggactacgtg cagcccctgc ggatccaccc gggagaagac atgcttgacg
     9361 cagccgtctt tgcctcctgc atccatggcc tgaagaataa ccagaatttt gtgcttgtgc
     9421 tgagcaaaaa gttttttttg cagttcctgc agttcttccc tcagtttgtc gaattcaatc
     9481 agggattctt ccttggttcc ggagaagaga gatttgtcat ccggagaata atcctttaaa
     9541 cgcagttttt tgcccggagg caccaggtaa cggttcatcg tacgcgaagt tagtgttgaa
     9601 gatgagggtg aggaggggga agatgttttc acagcggcgt gtttggaagg aaagccgggc
     9661 ccgaagaatt gctgaagggc gtttttgatg actttccttt tgaattttgg gaacatttcc
     9721 agtttgtgat tctggagggg aatccatttc gttttttaaa attcggggct gcggtgcaaa
     9781 tccgttttag ggcgtctggt tttgcagcgc acaagaaagt aggtttgttc ctggcctatc
     9841 cagcgcgtaa ctttacggtt gccggaaggg tatttgtaac gcaggccgga caggcggctg
     9901 acaatggtgt attccgtggg gcgcaggccc acttcctccc atacctcccg cgcaagagtc
     9961 tgttcaatgc tttcgtgttt aatgacgcct ccctgcggaa agtgccagta cgggttgcgg
    10021 ccgctgtcct tgcccagcaa tacgcaacct tcagcgtcca tgatgatggc tgctgcgttg
    10081 agtctccagt tttttgctgt gtgggggggg gccatgacag gaggaaaggc ttcttaatcc
    10141 gcggtttggt ccggtgttgc cggctttccg ttttccggcg caggggcttc cggttcatcc
    10201 tgccaaatga aatgttccag ctggatgcgg cgcgcgtaag ccgctgcttc cctggctctg
    10261 gggccggaag tctgcgccag tttttttgcc agtgccaggg ctccgtccca gtcctgaagc
    10321 tggcccagca ggcggatgga ggcgaagccg gcgttgtaga accagtgcca gtccctgcgt
    10381 ttgttggcgg aacttccgct gggcgtatgg gccagaatgg aggcatagtc atccagcgcc
    10441 tttttttgtt cgttcattcg ttcatagaac tgggcccgct gggtcagagc cttgaatttc
    10501 caggccagtc ccaggttggg cgtgtccagg atttccgtac agaggttgat ggccttgcaa
    10561 agagtgtccg aatgggtatc ttccttagct acccaggcat ccgcctggat ggagagggcc
    10621 agcaggcgca tgtagcgcgg caatggggag gaaagcaggt tttccagggc ggcgatgcat
    10681 tggtccgctt tccccatgcg cagcagaacg gaggcttctt ctattctggc aggctgggca
    10741 aagggggtgt cccccgtgcc tagggttttg aagatgttca gggcggcgtt cagcgtgctg
    10801 ccggtattgc tttgctgggc ggcttttcct gccagaaaga gggctgccgc ctgaagaggg
    10861 ctgtcagggt atttggaggg gaagggctgg aggacaagct gcgcttcatg gaagtctccg
    10921 tttttgtaca gaagctctcc cagtttgagg taaagcatat ccgcctgttt gccttccgaa
    10981 tccagggcaa tggctcgtcg gcaggactga atggcgttgg gccattcctg ccggctttcc
    11041 gcctccagaa tattcagccg cgccagctgc atggtctgtt ccggagacaa ctgctctttt
    11101 tccaacaggg gaaggatagc ctgcacggtt tccagatccg gagggttgag attcagggct
    11161 acttccacct ggtccagccg cgcctgggtt ttcagggccg gagtttccgc cagagttatg
    11221 aaatttgcca gcaattcagc agcctgaggg gccatccttt tagcggcgta atgggcctgt
    11281 tcaaagagga tgctctcccg gagccgggaa gagcctgcgg cttccttgat gagagcggca
    11341 gctccggagg cgtcattggc gttcagggcg gccaggtttt cattgaacaa ggcgttttcc
    11401 gccgttgttt ccgaagcgcg gcgggccgct tcccggaaaa gtttctgcgc cagggagtag
    11461 tcttttttga ggaaagccag ggctccctgt tgaaagacga ggtcagggga tgaaccggga
    11521 tatttggaaa taagacgttc cgcatcctgc agacggtttt tatccagcaa aacagtaatg
    11581 gtattcagag aaagaatttg aacgggaagg ctctggggat atttggagag cccctcttcc
    11641 gccattttga cagcgcctga cagatcatct ttttccagaa gaaggcttcc catggcatac
    11701 atggctgccg gctggcggga tgcatccctg gcttttaccc agccgttgag tttttccaga
    11761 gcctggggat tggttcgaaa gatgtcttcc tccagcagaa tgttgaaggc gtccatcagc
    11821 aaggcggatt ccgctttgcc gccaatgaac gccaacaggc gttcctccgc cgttccggcg
    11881 gtgtgcggag cttcctcctc ctgaggctgg gaccgcgttt cctcttccgc ctgttccgga
    11941 ttgctttttt cccgggctat ttccgccttg gcgagagcca ggcggcccag atcacgaatt
    12001 ttttccggat aggatttgtt gtccataacg gcggagaagg ttttgatggc ctggtccaga
    12061 tttccctgtt tgccggccag cttgccttct atgagctcgg cgtatgaacg tatgagccgg
    12121 ttgctgtccg tcccgtcttc cgtttccgtt ttgagttgtt caagaatagc ggcggcgttc
    12181 tggtaacatt cctgattcac cagtacatcc gccagaagaa ggcgcgcggg gtgggaaacg
    12241 gcgggggatt ccgcctgccc cagcctgctg agggtttcca gtaaaagctg ccggtcctga
    12301 agctggcgcg caagctgggc tttcaattga aacccgtaga gggaaagcgg atccgtttcc
    12361 gggatagcgg taagtttttc cagggctttg gtaaaggagc ctttattgaa gaggcctacc
    12421 gccgtccagt aggatttcat gggagagtcc ggcagctgct cccaggcatt cagtccttct
    12481 tccgtttttt tggcgcggat cagggcttct gccagaaaag ggcggattat ggctttttgt
    12541 gattccgcaa ggttttggga agacaggcat tcctggaagt gtttgctggc ttccagggga
    12601 aggcggtttt ccaaggcttt cagtccctgc tggtattttt tgtttttcag gaggtcctgc
    12661 ctgtcctgcg gaaaggtgtc tcccgcagag ggggcggcag ctgcagggga agcgccgccc
    12721 agggaaagca ggacagccgc gcaggatgtg agaacggcat ggggaaaacg gaagctggaa
    12781 aaggtttgca tcatcgtagg attatgattc aggccccatg aagatgagcc ggatgtagcg
    12841 tttgccggcg gcggtaagct tagggtcctt gctggagccg gaaacgcgat tgcaggtcct
    12901 gtacagttgg gagaaaggat gcggaacggc ccggatgacg acgggttccg tgcacggttg
    12961 gctggaagga gtcacggaaa cgggaacccc ttctcccgtg aaactgattt cccaggattt
    13021 ggggtcggag ggccccggct tcagcaggaa ggggtagcgt ttgacaaggc tgggcggttc
    13081 gccggaagag ggagaccgca cgacgtattg gaccggcagg gaggagatat ggcgagagag
    13141 ggaaaattcc gccccgtcct tgcattgcag gaggacgggt gccgggtcaa aaccggccag
    13201 attgagtccg ttataggaac cgtggcggtt gggagtgccc agtttcagac tggaatacca
    13261 gttttcaaag tcatcttgta gcttgaggca gagttccaga tgcacatgag cacgcgtttt
    13321 gttcaacccc accccggagt atcccagctt tccaataacg tttccggttc ctacccggtc
    13381 gcctttcctg caggagacgg aggccagatg ggcatacagg ctgtaaagcg ggccgtcctt
    13441 cagctggtgc tcaatgacca catagcggcc gtaattgcta tgggtggggt tggcggaggc
    13501 atggactacc gtgcctccgg ctacggggtg cacgtcgtcc agcggcgtgc cggaagcatc
    13561 cctcctgaga ggtttaatgt cgataccctc atgaaatttg acggctacgg ggcctgcctg
    13621 ggttctgacg agggtccggg taaagccgta ggctccagct tcccacggct gggatttctt
    13681 cccttcaaaa ttgcggtcta catacatgta aaagtcctgc gggcggttgt ccagcagggc
    13741 tgtattttcc gtggggaagc gcactacaat atccgccatc aggaatggca tgccccaaag
    13801 aaacaggagg atgccggtca ggagaggaaa ggatttgccg tgcatgctga tacgaggggt
    13861 gctggctcag atccgccccc ggcgggtgct gatcacggta cggtctttgt gttcttcagg
    13921 gtctttggcc tccttctttt cctgagcagg gtccttgctg ggaagaagag gcttggtaac
    13981 ggcggccagt ttgttgatgg gttcttctct cttgggatcc gccgggggaa taaattccgc
    14041 catggcggca agatccgccg gggagaaccc tcccatgact gccagttttc ctgaggtgac
    14101 aggtttgctg taaaggcgca ggggctgcat ggcatggccg ttgacgatgg gcagtatttg
    14161 cctgcccagg ttggaggcgg ttattccttc cacccggctg cccaggcctt tccggacgtt
    14221 gacgacgatg ccgtaggtgt tgtccggcgc agggaaggaa taataggatt caatgtcctt
    14281 atgggaaata aagggggcct tgctatagta atggccgtcc ctttcaaagg cgaatttttt
    14341 gctgtccgtc gctcccgtct ccatatgaaa gctgatggga tatttatccg gaccgcagga
    14401 gcagagcata agggaaccga agaggaacag gagcattttc atatgcgtcc atgatagaag
    14461 atttagttct ctgggggcaa accaataaac aggcatggag cgatttttgc cgtccttacg
    14521 gcagaaggga gagtgttttt tgaataatcc tgtccaccgg catgagtttt cccgcgcctt
    14581 ccgctccgca ggccagaatt ccggattcac tggggccgag gatgtaatga tccttccttt
    14641 gcttcagaac ggccgcgttt tgctgcgtgg ccgcatgttc ccacatgtgg acgttcatag
    14701 ctgggcaaat aagcaccgga gactggcaag ccaggtagag ggaactgagc atgtccggag
    14761 cgaagccatg ggcaaaattg gccatggtgt ttgccgtggc tggggctaca agcagaagat
    14821 ccgcccgttg ggccagttgg atgtgtgggg gaacccagtc cgtcttttca tcctcaaagg
    14881 aggaaaagac ggggttgcgg gaaatcgtgt gcagggtgag gggagtgaca aattcaaggg
    14941 ccttggctgt gcagacgcag tgcacttcat gaccgtattt gaccagggcg gaagcgatat
    15001 ccgccgcctt gtatgcggca atggagccgg tgacgcccag aatgatgcag gccatgaatg
    15061 ggaaaaggtc aggctttgga ggcgtttttg ccttcgtcga taagtttcca gaagtgcttg
    15121 gactggctca ggatttcatc ttccccggca gtcggggaaa tattggtgcg aaacaggaaa
    15181 tcgttcaggc tgttggcgtg caggacgcgg tggacgatca ctttgccttc gatgacttca
    15241 aagtaaatgc ggtaatcttt ggcgacgtag cgataaagaa ttttaccatc cttttccatc
    15301 ttgccgaaac gggcgtcatc cagggtctgg agggtggatt ctgagacttg gaagtctccg
    15361 agcagatcca gctgttccaa ggtggggatg ctggatactt ccgcagcgct gattttattg
    15421 aagacgattt gaagcacgcc cttttttaag tgcgccggcg ggcgagttca agcctgaaag
    15481 aaggttctgg gcgattttga gcgggagccg tccggagccc cggcatttac ttggagcccg
    15541 gatttggatt aacggggatc ctgcgtgggg aggattccct tgcttgttac cacctgggtg
    15601 ccgcggatga cttcgataga cagaggtttg tctcctgtta ggtaataagt ggcgtccagc
    15661 atgtctccgt agttgcggat gggcgtgtcg ttgatgttga tgaggatgtc cccggtctga
    15721 attcctcccc gtgcaagagg acctccgttg ataaggccct caatgacggg ggttgaggag
    15781 ttgatgtcca gcaggcaacc caggcgcact cttttggctt ccggatgggt tgccaggaag
    15841 gagatggcac gggcgggcag caggtggcag gttcctttgc gggaaacgcc cagaacaatg
    15901 ccgaccagac ggttttcaga gtcgtagcat ggttggccga tgggggggac tgccagcttg
    15961 gggaactggg cgcggatgag ccgcaggggg aaactggtgt ccccgatgga atgttccatg
    16021 cccacgtaaa ttccctgcgt ggtctcattt ttgctgctgg caaaggacag cttgtcgccg
    16081 cgtttaagaa tgccgggagg ggtcaggtct gcctgtccgt cgtatatatg ggctggatcc
    16141 accttaaaga ttgtcaggcc tgtgagcttt tcctgggccc aaacctgaag gggcggggta
    16201 ctcttgtccc cgaggtggta ggtcacgcct ccgtttttta aaacccggta gggaatcaag
    16261 gaggccagca tgtctttacc gatttgcgcc gccatggtcg catacggcgc cccttccgcc
    16321 tgggggagga tggcttctac cttaaaaggg acaggcacgg ccgttgcctg gcaaatcagg
    16381 cctgcaacgg ctatgcccgt caatatcgga gccaagtcca tcatggattg ttacatctca
    16441 atgagaacgc gggataccgg ttttttatcg gcatgggaat caggtttcag ctggttgtct
    16501 ttggccgatg tttcatatga agagtctccc gtggtgaatt caggtttttc cgtgggagca
    16561 acttcggttg aggttcgttc aatagtttcc ttacttacgg gaacggcttt tttcgtcagg
    16621 agtatttctt ccttatcctt ggtggaggat acgtaagatt cagaaggttc atccatgttt
    16681 ccggatgcga taataacgcc taccgcagcc aatgtcacga tggacatgga agcatagacc
    16741 cactgccaac cacgaaagtt ctgcagataa ttgtccaggc gttcgagcag caatttccaa
    16801 gcagagtggg cagcctgatc ggtttccttt ctgagatgaa aattgcggag gaaatcttcc
    16861 tcgaaggtgt catggtaaca ggcttccttg cggaaagatg ccagcatggc caccagagtt
    16921 tcctctttat gatctgtgga ttgtttatcc atcgtcgttg atgttaagta taatagaagt
    16981 tagaatgtgt cagtcccgaa tttgggacta tgcgtttgac atggcggcgg gaattgacgt
    17041 tcaatatttt ttttcctgat taacttagat attccgccag cagcccctgg agttccttgt
    17101 gggcatagtg cagacgtgat ctgagcgttc cttcagagat accgaggagg gcggatattt
    17161 ccgtatggtt gagccctttt acgtcatgaa gaacaacgac ttccctgtgt ttagtggaaa
    17221 gtttcttcaa tgcttcgttg attttgagct gcaattcatt caaatgggta cggcgttccg
    17281 ggtccgcccc gtccgtttca tccgccatgt tcatgtcctt ttccgtatgg tcgccgtact
    17341 cgtcgtcgtc cagactgtag gtcattctgc ggttgcgttt tttcaggaag tttctggcgt
    17401 ggttgacggc aatggagtgc agccatgtgt aaaaggcgct ttgtccattg aagtagcgca
    17461 aggcccggta ggctttggag aacgcttcct gggcgatgtc ataggcatcg tcgtagttgt
    17521 ccgtcatctg gtacagggta gcatggagtt tgcggctgtg gcgaatgaca agttcgtcaa
    17581 aagcccgcga atctccttcc cgtgccctgg cgaccagttc ggtgtcttcc aaggtcgtgt
    17641 atgaaatggg tcccgacatg ttgtcgacag aatacccaaa aaaagggagc cgctacaagg
    17701 gcaaatcgta tgcacggccc cctttaccct caaaccagcg catgtatgcc acattaactg
    17761 tagtgtatcc gcccggagtt gggtaatctc cggtgaaata ccagtcgccg caggggcctt
    17821 ctatggattc atggaggttt tcgatggtct ggaaaatgac ttccacgggg cacggagcgt
    17881 catgcggagt gaccagacgg gtgatttccc tggagatttc agcctccgtc aacccttcgt
    17941 aaatagcctt cacacaattg cgccgttctt ccttgggttt ggccagttct tccctgcatg
    18001 ccttgtatac ttcttccagc agacgggcct gtccgtgctg cttgatcagg gagacggccg
    18061 cctggaaggc gatgaagttg cccagttcgg acatgtcaat gccatagcag tccggatacc
    18121 ggatttgcgg ggcagtggat gcaatgacga ttttccgagg attggttcgg cccaggatgc
    18181 gaaggatgga ccttctcaag gtggtgccgc ggacaatgga gtcgtcaagg gcgacgagca
    18241 cgtcttccgg ccctacggcg ccgtaagtaa ggtcatatac atgggagacc agttgcgcac
    18301 ggcttttttc ctgcgtgatg aaagtgcgca ttttgatatc cttgtgggcg attttttcac
    18361 cgcgcggcca gcgtttcagg atagcgctgt ccagcatgtt ttcatccagg gtgccgtttc
    18421 tcagggcttc cagaagctgg gcatgcacgc gcttgcggcg gtagacgcgc aggccttcca
    18481 gcaggccgta gtaggcggtt tccgccgtgt tgggaatgta ggtgatagcg gatttgtcaa
    18541 aacggtcttc cagagaatcc acgatttggg gggtcaatgc cgcaccgagc gctttgcgtt
    18601 cccggtaaac gataggatcg tttccgcggg agaagtaaat tttttcaaag gagcagggag
    18661 ccggtttcag aggtgtggta aattccgtaa tggcatgtgt gccgtctgcc ttgatagaga
    18721 gcatgttggc cggcggaagt tcttgaacct gttcgctttc cacttcaaaa acggtcatca
    18781 gcggaacgcg ttcggaggcc acggcaatga attcatccgt aatcagatag tggcatgggc
    18841 ggatgccgtg cggatcccgc aggcagaagt agtctccgtt accgatggct cccatgatgg
    18901 catatccgcc gtcccattgc ttggcggagt tatggataat ttcctgaatg ttgagccggg
    18961 aggaaatggc atggggtatt tccgggccgg gcatgccgct gtcccgcaag gcgcggtaga
    19021 ggtccgtgtg ggcttcatcc aggtgatacc ctatttcttc aaggacagtc tgcgtgtccg
    19081 ttccgaagac ggggtgctgg ccgcgttcaa tcatgcgctg gttcaattcc ggcgtattgg
    19141 tcatgttgaa gttgcccaga accatcaggg tacgggtcgg ccagttggtg cggcgcaggt
    19201 aggggtggca ggacccttcg tcaaagaggc cggaggtgcc gtagcggaga tgccccatca
    19261 ggatttcccc gccgtagtcg aagttgtttt tgatatctgc cgcatccttc agattgacct
    19321 ggccgcggcg aactttttta ttgagcttct tgatttgccc gttgaagatg ctcgtgaggg
    19381 cgtctttgct ggcgtcccgg gtgcggaaca ggtaaggctg ccccaagggc atgttcagct
    19441 tgacgcagcc tatgcccgcg ccgtcctggc cgcggttgta ctgcttttcc atcaggatga
    19501 gcagtttgtt gaacgcccag agggtggagc cgtatttatc ctgaaaataa gagagaggct
    19561 taagcagacg aatggcggct acgccgcact cgtgtttaag aaaatcgctc atgggatgat
    19621 gatgaaaaga ttgtcctgta agtgtggtgt atccgctgca aggagatggt gttttttatt
    19681 cgccctgtac cttgacatga atgccgcggc cttctctcag cgtgccaatg accgtgccgc
    19741 ccggacctgc cttcaatgcg gcttccgcgt cttcaggcct cacgatggct acccagccaa
    19801 tgcccatgtt aaaagtgttg aagcggtcct gaggatccac atgcttcagg attttctggg
    19861 cggcggtgtt gtcccaatag ggaatggaga ggtctgcgcc gtagtcgccc aggaagcgtt
    19921 ccaggttttc cgggaggcct ccccccgtga tgtgggcgta tgctttcggg gtgacgttga
    19981 agcggcgcat atcttccact acgtcatgat acaaacgggt gggctgaagg agggagcgga
    20041 gttcttcttc atcaactaca tccggatttt cttccagaat gcggcggatg aggctccagc
    20101 cgttggcatg gaagctgtcg gatttgtagc cgatgaatac gtctccgggc tggacggttt
    20161 tcggatcaat cagtttgctt ttttcacagc agccgatgca gaagccggag agttccacga
    20221 tggattccgg caccacgccg ggcatttccg ccgtttctcc gccggccagg atgcagttgc
    20281 aggattccag gtagtcgcac atgccggcca ccaggcgggt gatacgggag cgttcccgtt
    20341 ccagattgga gatgcccaga taatccagga aaaggagagg gtcgccgccc gtagtaagaa
    20401 tgtcgttgac gttcatggcc accaggtctt tgccggcggt ttccaccatg tccagttcaa
    20461 aaagaatttc cgtcttggtg cctacgccgt cgcacccggt gacgatgacc ggttccttgt
    20521 aggagctcaa atcataggcg gcggcgaaaa ggccaaaggc ctggtgcaga gatctctgct
    20581 tttgcgttct tttaacgtga gagctgatgt cggatacaaa agcggctgct tccttggtat
    20641 cgacccccga ttgcttgtag gttagtttgc cggacattga acggatggac tgggtgtgcc
    20701 agcatcctag aggaaggctc cttccgcggc gagtgaaaaa agaagtattt ttgccatagg
    20761 acgcgcggga ggaactgttt tcattccact tctatcttgc agtcgcgcca gagccaccag
    20821 gcatccaggg agaatttgcc cggtcccgta tagaagaggg tcagatagat cagcaggtag
    20881 atggccgcta gttcctgtgc tccccagccg gagaggccaa ggacaagaaa gagcgccaca
    20941 cacatgttaa taaccaggat aagggaggcc aggcgggtca ggaacccaag caaaatgagg
    21001 atggagcaac cgaattccgc acctacgcac agcagcaggg acagaaaatt gccgattccc
    21061 agaggatcct ggaaggccga ccagtttccg ctgatgagca tgcccagttt gggaatgcca
    21121 tgcacaagca tcatgactgc tactcccagg cgcagcagca gaacgccgaa atccatactg
    21181 gtgttggtgg ccaaggggcc aagcaggcgg catgcggatg gtttgctttt attctgttcc
    21241 atgcacggta gaccgttttg gatgcagaaa tgctcaatta aaataggatg aaaaggagca
    21301 tccgggatag cggggaagct gtcccagacg caggcattcc gtttgcgggg aatccggatg
    21361 tcccgcagag ttccgcagct tgccgcctgc ctcggtgcgt aagggcgcga atagagtgcg
    21421 ccgggtattt gggaacatac ggaactgctg catgttccca atgaaactgt tccagtggtt
    21481 tttaatattt ttttgcggga atgaggcgga aagctgcggg ggatttggcg gatttgattt
    21541 gcaggggcct ggtcgggtct ttttgggtct ccgggccaat ggcgatgata agcctgtcct
    21601 tgggatggat gacggtggag ttttcaaaaa tgagcgtatt ggagccgtcc gtcaatttcc
    21661 atccgtccag aagaatgccg gaattaccgg ggttgttgat gatcacgttg ccggtacccg
    21721 gttccagcct ggggacgggc tgcttaatct tgaggctttt tgccagcttg gcgaaggtga
    21781 tttccgcaat acgccgtgcg ccctgttccg taggatgcag cccgtcaaca taccattccg
    21841 gatggtcgga gagcgggtgc agcgcatcaa agagactgac tttgtacttg cgcgctacgg
    21901 cggcaatcaa tttttccgct tccgggcggt tggcggcgga gccgttgtcc gagcctgcat
    21961 atttgcgtac gtccggatag cagttgccgg ggaatgcctt tttgttgagc gggccgcggt
    22021 agtccggccc cagcaggccc caggcaaaga accgtgcttt gggattggcg tttttccatg
    22081 cctggagcag cgcttcatag cctttggaga acagttcggg gttccaccat cggcctgtgt
    22141 cgttgatgcc aaggttgcaa atataaatat ccgccttgaa gtccagggct tgcttgtgtt
    22201 ccctggtgct gccgtaccag cgtcctgctt ggcccggata gtcgcccgcc gtttttccgg
    22261 gattgccgaa tcctctgacg tcaaagtccg ggcccattag cgtggcgagc acttgcggat
    22321 accgggcttc tccgggttta aggccggagc cgaaggtgat gctgtcccct acgcaggcca
    22381 ctttggcggg agaagcttgt gccgcaatgc ccagtgagag cgtggctgcc agggaagcaa
    22441 ggaaaagatg aaggttcatg gaatgatggc gtggtaagag ttattttttc agaacgggtg
    22501 tgtccgtccg gaagggacgg gcggggagtt ggttgccgtt gaccaggttg ggggtcacga
    22561 aacggttcca gcaataccgg gcgtttttgg gggattttac ttccggggag gagaggcgga
    22621 taacggctgt gccgcctttc ctgtttgtga tttccgccgt ggcgggatgg taaattccgt
    22681 ccgtcccggc aatttcaaac tgggccggtt tcttgccgtc cgtggtggtc agtccggcag
    22741 cttgttccat ctggaccagg atgctggaac cggaggtctt gaaggccttg aaggcagggc
    22801 cttcacaggg gaccttttta ccgtaaacct tgttcagggc agtatttcct gcacgttccc
    22861 caacagggcg cttgaacgga ggatgaacgt cggcattggt ggagccaaga tcaatggtgt
    22921 tgacgctgta aacctgcggg acggttttgg ccactgtgtc ctgcatttcg cggaattccg
    22981 gccatccggc acggattttt gacggatcgt taatgcgggg gagctggatc atgacgaagg
    23041 gcatttccgg gttacggaaa gcttttctcc aggaggagat catagttttc agcaacatgc
    23101 cgttttgggc gttgtcaatg atctccgcat cggattcgcc ctgataccag atcacgcctg
    23161 tgatggggag tggtgttgtc caggcaatgc cggattcgta aaggaacgcc ggcttgtagg
    23221 gatggtccgg agatccctga ttcagacgcg gagagatatt ttttctggcc cgtcccctta
    23281 cccagtcgga gataaggggg gagtccagcc agtggttgcc gcgcagggtg cggaaactgt
    23341 tgtttgcatt aatgacctgc cgggggagcc acgctgcgat ttccgagccc cccagggagg
    23401 agtggatgat gcctaccgga atgcccagcc cttcgcggag ttttttcccg aaatagtagc
    23461 ccacggcagt catggggcct gaagtggagg gtgagctggc ggcccattgc cctttataga
    23521 agttgtccgt ggtgacggcg tcaaaatctt tcccggagta gggggcgttg ttggtgtggg
    23581 cttgcggaat attgttgagc aggcggagct ggtcatctcc ggaagccgct atgtcttctt
    23641 tggccgtcgt ggtctggttc aggcggaaca gcatgttgga ttggccggaa gccagccaca
    23701 cttctcccac gagcacatct tccaatttgg cggagtctcc gttttgcacg gccgccagcg
    23761 tctggcctgt gccgcaggga ggcatgggag gcagcgtgac gctccatttt cctttggaat
    23821 cggtttttgt tttcagtgca gtgtttccaa aagtgacggt tatttccctg ttgggggcgg
    23881 ccgtgccgga aacgggaatg tttttcccgt ggggaagtac catatgggaa ccgaaaatcc
    23941 ggtcgaaccg gggggcgtcc gcccacgctg cgttaagggc gatggcggca gccaggatgc
    24001 atgaagtatg gaggcgcatg gtttgaggat tatttgagaa ggagttcgtt ccgtgagagc
    24061 ccggtcagcc tgccgagctg tttaatcagg cgggtcatga tgaagacaag agctgccaga
    24121 atggggatcc cgatgatcag gaagccccac caggtgatgc tgccgatagc ggcattgtag
    24181 gccacttgct gttccgcttc cggctggcgt atgacggagg acatgaaaga gaggctcagg
    24241 aagaaattgc cgacggagga tgcgaccaat gtgcccgcca gaatccatga cgagtttgcc
    24301 agcaggctct ggtaagcctg ttcctcgccg ttggcggcca ccgtttgttc tatcctgcgc
    24361 acgtcgaata actccgtcgt gtagatgaac atgttgagga ggggggtgcc ggtggagcgg
    24421 gagacgacga cggccgccgc caggatgagg gggatcaggg cttcttttcc ggcgaacagc
    24481 cagggtgtgg cgctgtggat gcgcccttcc ggcccgatga cgaagatgct gatgacgccc
    24541 gtcagcagca cgccggccat gcctacgccg gacatcaggt caaatttcct ggagacggcc
    24601 agggaacgga tgccatagac gagtgggagg gaaagggcga tgacgagaga ccataccggg
    24661 ccgatatgcc agaaactttc tccctccgga cgttccaggg gattggcggg gcctgcgctg
    24721 cagtagtcca gaatcagtac cgggaggagg atgttgatga aaattcccag cagggaggac
    24781 tgtgatttgt ttggcgtttt atccatgaat tcggagggaa atatttgtat catacgcgaa
    24841 tgcgcccctg caagttgcaa ccccgtgtgt gacagtaaag accagaagcg gggagagccg
    24901 tttttttatt cgcaggcggc tttgcggaaa tcgtggcatc tacggtatct tccgttcttt
    24961 tggctggaca cgggagggaa taatgttcag cttctcccgt gactctgaca gatcattttt
    25021 cccccatgac tcaaacatct tctttttcgg ctcttggtat ttcaccggag attctggagg
    25081 ctatcgaagt gctcggcttc gatacccctt cctccattca ggagcaggcg attcccgccg
    25141 cgatcggagg ggccgatatt gtggggttgt cccatacggg ctccgggaag acgcttgcct
    25201 ttgccattcc ggccctggag tgcattgatc ctgaggaacg ctgcgttcag gtgctggccc
    25261 tctgtcctac tcgggaactg gccgttcaga tttgccggga ggtggacaag ctggctttgt
    25321 tcatggacgg tgtttccgcc gtccccattt acggaggctc ttctttccgc ccgcaactgg
    25381 acgccctgag gcgcggggta cagtttgtgg tgggaacgcc cggacgcgtc atggacctga
    25441 tggagtccgg ggcgctaagg ctggacggtc tccgcatgtt gattctggat gaagcggacg
    25501 agatgctgga tatggggttt ctggaagata tcgaacgtat cgcggacgct atgccggaag
    25561 ggaagcagac cctgtttttc tccgctacca tgtctccgga aatcagccgc ctggtgagca
    25621 ggttcatgaa ggagcccgtc cagatttcca ttgaacggcc tacgctgacg gtgcctacca
    25681 ttgaacaggt ttattacgag gtggtgtttt cctcccggat tgaggtgctc agccggctgc
    25741 ttgacaccgg aaagattaaa atggggctgg tgttcgccaa taccaaaaag gtggtggatg
    25801 acgtggtgga cggtctgacg gcccgcggtt atccggtgga ccgccttcat ggggatatgc
    25861 cccagatgat gagggagcgc gtgatggatt ccttccgcaa ggggagcctg cgccttctgg
    25921 tggcgacgga tattgccgcc cgcggcctgg atgtggacga tgtggatgcc gtggtgaatt
    25981 ttgaattgcc gcgtgatccg gaggattacg tgcaccgcat cggccgtacg gcacgcgccg
    26041 gccgcaaggg gaaggccatt acttttgtgg ggcgccgtga tttttccctg atgagccgca
    26101 ttgaacgctt catcggcgtt aagttgactc cggaacctgt gttgactgcc gttcaggtgg
    26161 accagctccg gacagagtct ctggtggatg atatcctgga acgtctcaaa ccggatgccg
    26221 tgctgccgga ggaattgaag gaagtggagg ctgcgccgga ggccatggct gcggccctgt
    26281 ttgacttgct ccgggaacgt acgcaccggg aagtgcagtc cattccggaa gacaagcccg
    26341 cccgcagggc gcggcgtatg ccgcagaccg gggaggaggc ggagagcccg cagaagggtg
    26401 attcctggca gcataagggg gatacggtta cgctgtttat gaatggaggc cggatgattg
    26461 gcttgcggcc caaagacata gccgggttat tttacaatac ggtggaaatg gaaaagggag
    26521 ccgtgggcga tatccggatt ttccccaagc attccctgat agaagtggac gcttccgtgg
    26581 cgcccctgct tttggaggcc ctggcgacgg ctaccgtctg cggctatgaa gttaatgtcc
    26641 gtgaagacaa gggggctccg gaatattccg gaactccgcg ccctccccgc cgcaatggag
    26701 gattccgcaa cagttaccgc ggagaacggg tccgcagtga ccggagcgga ttcaggggaa
    26761 accgcggggg cttccgcagt gaacgcggcg aacgctgggg agaacgttcc cgcagggacg
    26821 acaggaagaa gcgtttctga tgccttcgct attccggaca gggttattaa cgctgttccg
    26881 gaattggcgc ggacattttg gaacgttccg gtttatgatt gctgtttccg ccggtttctg
    26941 tattttctgc ccggcgggga ctgccgtttt attcaggata aagcatgacc gggcgggccg
    27001 gggcgccggg aatgcaggag gaattttctc tcattcatgg cttcagccag aaaatccgct
    27061 cattggtgca gtatggcggg ttgtagttgt tccggctgtg aaggctgggt ccctgatgtt
    27121 ttttgccgat aatactggcg attcaatcct ctgtgccctt tcctcgtaat tttcttgaga
    27181 gatgagaaaa ttcatgtaag atgacgcttt gttttaacgt cttttttata gtctattttc
    27241 tttg
//
LOCUS       DAWEMX010000016        27045 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_732_length_27045_cov_12.830085, whole genome shotgun sequence.
ACCESSION   DAWEMX010000016 DAWEMX010000000
VERSION     DAWEMX010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 27045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27045
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_732_length_27045_cov_12.830085"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7350..7359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10326..10335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5380 a         6953 c         8209 g         6483 t
ORIGIN      
        1 ttatattgca tgggacaata ttggaatcat cgccggattt tcaatccgct tttccttttt
       61 cgtggaataa cgcggagagt ccataaaaaa ggccgcaccc cggaaacggg gtgcggcctt
      121 tgaagtgtgg gtggagagaa caggttagcg ttcgttgtcg aagttgatgg tatcgttcaa
      181 catgtcttcg gcaagttccg ccgcttcctc ttcttcgctg gccgacgcga tttcttctgc
      241 gccctctgca ggttccactt caatcttgct gtaacgggag aatccggtgc cggcgggaat
      301 gaggtggccc ataatgacgt tttccttgaa gccttccaga gtatcggtct tgccgagggt
      361 ggatgcttcc gtcagaacgc gcgtggtatc ctggaaagat gccgcggaaa tgaaggattc
      421 cgtttccagg gaggccttcg tgataccgag cagaacgggc ttggctgcgg ccggttggcc
      481 gccctgggct acggtttgtt cattgatgcg gtcgaacgtg gtcttgtcca cttggtctcc
      541 ccacaggaat tcggtatttc ctggttccgt gatgactacc ttgcggagca tctggcgcac
      601 gatgatttcc acatgcttgt cgttgatttc cacaccctgg aggcggtaca cttcctgaac
      661 ttcattgacg aggtgttcct ggagacgttc cttgccgcag acatccagaa tttcttccgg
      721 agaaacgggg ccttccgtaa gctgatcacc cagatgcaca tggtcgtctt catgaacgat
      781 gacgtgcttg ttcatcggga ccagatggtc caccagttcg cccgtgggtg tttcaatggt
      841 aatgaccttc ttgccgcggg aggtattctt gctgctgagg cgcacaatgc cttccacgcg
      901 tgcaatggtg caggcgtcct tgggcttgcg ggcttcgaac aattccgcca cgcggggcag
      961 accgccggtg atgtccttcg tcttggctac cttgcggggc gtcttggcca ccattgtgcc
     1021 ggcggagatg gtttctccgt ccttcacggt gaggttggcg cctgcgggaa tggcatgatg
     1081 agccagaact tcgcgggtct tggcatcgcg gatgaccacc tgcgggtgaa gttcctgctt
     1141 gtgttccatc acgacaaggg aggaggcacc ggtttcccgg tccgtttcct tggaaacggt
     1201 gatgccgacg atcatatcct tgaattcgac gatgccgccc ttttctgcaa tcacgggcac
     1261 attgtacgga tcccaggtgg cgagggtttc atccttcttg atagtgccgc cgttgggcac
     1321 gtacaggatg gtgccgatga cgggctggta ggattccagt tcgcggccct gttcgttttc
     1381 gatgcggacg gagcagtttt tattcaggac gacgaagtta ccgtctgcgt tttccaccgt
     1441 gcggagatct tccgtgtaga taacgcggcc gtcgttcttg gctttcacga tgggctgctt
     1501 gaacgccgtg gtagccgttc cgcccacgtg gaacgtacgc atggtgagct gcgtgccggg
     1561 ttcgccgatg gactgggcgg caataatgcc gaccgcttcc ccgatcttca cttcctgtcc
     1621 ggtggccagg ttcaggccgt agcacttggc acagcagccc tttttgagtt cgcaggtgag
     1681 tacggagcgg attttcagcc gttcaatgcc gattttttcc agttgttccg cctgcttttc
     1741 gttaatgagg tcgttgatgt ctacgataac ctcgccgctg acggggtcaa cgatgcgttc
     1801 gcaggaagtc cggccgtaga tacgggagga aagggacgcc acttcttcgt cgccgtcata
     1861 gatggcgtga acggtgatgc cgttgctggt gccgcaatct tccgcatgga cgatgacatc
     1921 ctgggccacg tccacgagtt tgcgggtcat gtagccggag tccgccgttt tcagcgcggt
     1981 gtctgccaga cccttgcggg cgccgtgggt ggagatgaag tattccagca cggaaaggcc
     2041 ttcacggaag ttggccgtga tggggcgttc gataatttcg ccgctgggtt tcgccatcaa
     2101 accgcgcatg ccggagagct gcttgatctg cgccttgttg cctcgggctc cggagtccac
     2161 catcatgaag agcgggctgg ccatcttgga gccttcgttg tgttccagct tgcggtacag
     2221 agccgcctgg atgacatccg tagtctgggt ccagatgtcc accacctttt ggtagcgttc
     2281 cccatccgtg ataataccgt tcttatactg gcgggtcacc ttgtccagct ccgcataggc
     2341 tttttcaatt tccgtctttt tctgggaggg aaccaccatg tccacgatgc cgatggagca
     2401 gccggaacgg gttgcttcct tgaagcccag gtttttcagg gcgtccagag tctgcacggt
     2461 acgttccttg ccgacggtct ggtagcagcg ccagatgatg tcgcccatct gtttcttgcc
     2521 tacgttgcgg ttaatataac cgagttcccg gggccagatt tcattgaaac gcacgcgtcc
     2581 ggcagtagtg atgataacct tcttggtgac atccccatac actacttcgg aaggcttttt
     2641 gccgtagtcc gggttgtgca ggcggatcca gtcatggtag ccgattttgc gggcggcaat
     2701 ggcgtattcc acctcgtcaa tggattcgaa gaggggaaga tggtgatgat tatcctgatt
     2761 gttctgtact tccgcagcac gggtgtgcgt caggaagtac gcgcccagaa taatgtcctg
     2821 cgtgggtgtg gcaatgggtt tgccggaagc aggggagaaa atattattgg gcgccagcat
     2881 gagctgccgg gcttccatct gcgcttccac ggacagaggc acgtgcacag ccatctggtc
     2941 gccgtcgaag tccgcgttgt acgcattaca gacgagcggg tgcagacgga tggcggaacc
     3001 ttcaatcaga accggttcaa aagcttggat ggagaggcgg tgcagggtgg gcgcgcggtt
     3061 gagcatgacc gggtggccct tggtcacttc ttccagaata tcccacactt ccggcgtctt
     3121 gcggtcaatg agcttcttgg cggagcgcac cgtgtgcacg taacccagct ctttcagacg
     3181 gtggatgatg aagggttcaa acagaatgag cgccatcttc ttgggaagac cgcactggtt
     3241 gagtttcaat tccgggccga tgacgataac ggagcggccg gagtagtcca cgcgcttgcc
     3301 gagcaggttc tggcggaaac ggcctccctt gcccttcagc atgtcggaga gggatttgag
     3361 ggggcggttg ccggcgccgg tgacggcacg gccgtggcgg ccgttgtcaa acagggcatc
     3421 cacggcttcc tgaagcatgc gtttttcatt acggatgatg acttccggag ttttgaggct
     3481 caggagggtt ttcaggcggt tgttgcggtt gatgacgcgg cggtacaggt cattcaggtc
     3541 ggacgttgcg aaacggccgc cttccagagg aaccagcggg cgcaggtccg gaggaatgac
     3601 gggcagaacg ttcaaaatca tccattccgg gcgtgtgttg gactgaagga acccctgggc
     3661 cagtttcaga cgcttggcaa tcttgcgttt gttctgtttg gagttggtat tgtccagctg
     3721 ttcctgaaga tccgcgacga gggtgggcag atcaatggcc gccagcattt tctggatggc
     3781 ttccgcaccc atgccggctt caaagctgtc atagccgtat tcgtcttccg cattgcggaa
     3841 ttcttcttcc gtcaggatgg cccccttttc cagaggggta ctgccgggat ctaccacgat
     3901 gtagtcttca tagtaaatca cgcgttccaa atggcgggct gtcatgtcca gcataaggcc
     3961 aatgcggctg ggcatgcatt tgtaaaacca gatatgggaa accggaacgg ccagttcaat
     4021 atggcccatt cgttcgcggc gcacgcgcgc gttggttact tccacgccgc agcggtcgca
     4081 ggtgatgccc ttatgcttga tgcgcttgta cttgccgcag gcgcattcca tgtctcgggt
     4141 gggcccgaag atgcgttcgc agaacaggcc gcctttttcc ggcttgaacg tgcggtagtt
     4201 gatggtttcc gggttgacga cttcaccgaa tgaccagctg cggatggtat ccggatccgc
     4261 cacggtgatg gcaacctggt caaaggtccg gggcttgtcg ctcaggccgt gcatttccct
     4321 gatggtgggg gtgtcagaca tatattgaaa gtttaaagga tgtttgatgc ccgcccccgc
     4381 gttttgcggc acgggggcgg aatgcggtgg atattagaat ttgaggtcgg agaagtcgac
     4441 gtccgccagg ccggccatgt cgtcgctgtc aaagccttcc gtcatgccgt ccacgggagt
     4501 gagatccgtg ccgccaagtt gcagggaggg ttgttcatcc ttgctgccgg ggcgtacgtt
     4561 cagccccaag gactgcattt ccttcatcag aacgttgaaa gattccggag ttccggcttc
     4621 cagggtatta tcccccttca cgatggattc gtaaatgcgg gtacggccct gaacgtcgtc
     4681 ggacttgacg gtgagaagtt cctggagggt ataggcggcg ccataggctt ccagtgccca
     4741 cacttccatt tccccgaaac gctgaccgcc gtattgggcc ttgccgccca gaggctgctg
     4801 cgtgaccagg ctgtacggac ccacggcgcg ggcgtgaatc ttgtccgcca ccaggtggtt
     4861 cagtttgagc atgtaggtct ggcctaccac caccgggtta tggaaaggtt cgccggtcag
     4921 gccgtcataa agggtactct tccctcccac ggtgccgtcc ttgccgtcac cgatccaggt
     4981 gaaaccgtcc accttcttgg cttcggacat gtaattccag atggtttctt cactgatccc
     5041 gtcgaacacc ggggtagcca ccttgaagcc aagggccctg gcagcgatgc cgaggtgggc
     5101 ttcaagcacc tgccccacat tcattcggga aggcacgccc aaggggttca gaacgatatc
     5161 caccggagta ccgtccgcga ggaagggcat atcctgttcc gggacgattt tggcaacgac
     5221 gcctttgttg ccgtggcgac cggccatctt gtcacccacg ccgagcttgc gcttggcggc
     5281 gatatatact ttaacttcct tcagtccgcc gggttcggat tcgtccccgg attccatctg
     5341 atccagcctg tgttcccgtt cgtcatccag ttccgtaaag cggccttcaa acgaagtgat
     5401 gatttccaga atcttgttgc gaatcgggct gggttcgatt tcgatgtggt cgtgaaccag
     5461 ggccagttta cgcaacaggg tcttggtgat tttcctgttg gccgggatga tgatttcacc
     5521 ggtctgttca ttgacgacgt ccagcgggat tttttcaccc agaagaatat cggagagctt
     5581 cttggtcaac tggtcaatga gctgctcctt cttcttttta tgttcgtcgt tgattttctt
     5641 gaactgcttt ttcttttccg cgctgtctac gacgaggtcg ccgcggtggt ggccggagcc
     5701 tgtggaagaa atgcgcacat ccatcacgat gccggtgcag ccggagggga cacggagtga
     5761 ggtatctttc acttctgcgg ccttttcacc gaaaatggcg cggaggagac gttcttccgg
     5821 agccagttcc gtttccgatt tgggggtgat tttgccgacg aggatgtcgc cgggcttcac
     5881 ttccgcgccg atgcggatga cgccgtcatg atccaggttt ttcagggctt catcaccggc
     5941 gttcgggata tcacgggtga tttcttccgg acccagcttg gtgtcacggg cctgaacttc
     6001 aaactcggag atatgaatgg aagtgaaggt gtcttccttc acggtttttt cggagatgac
     6061 aatagcgtct tcgaagttgt aaccgttcca cggcatatag gccaccagaa cattgcggcc
     6121 gagagccagt tcgccctggt ccgtgttcgg gccgtccgcc agcacgtcgc cggttttgat
     6181 tttgtcgccg cggcgcacga tcggacgctg gttgatgcag gttccggcat tggaacgcat
     6241 gaacttgcgc aggggataga cgtaaacgcc attgtcgcgg tccgtgcgca cactgtcggg
     6301 atcggacagg aatacttccg gacgcacggg cagttccccg tctttcgtgg tgataatgac
     6361 ttcagccgtg gcggaggcca cgatgccgtc cgcttccgcc agaacgacgg aacgggagtc
     6421 cctggcgcac tttccttcga tgccggttcc cacgtatgga gattccgcta ccatcagagg
     6481 cacgccctgg cgctgcatgt tggagcccat cagggcgcgg ttggcgtcgt catgttccag
     6541 gaaggggata agaccggcgg cgatggaaac gagctgcttg ggagaaacgt ccatgtaatg
     6601 cacgtctgcc ggatccactt cgatgaattc gcctttttca cgagcggtta cgcgggaggt
     6661 cgtgaagttg ccctgttcgt ccagcgggtt attggcctgg gcgatgagat agccttcttc
     6721 ctgatcggca gtgacgtatt cgatggtatt ggtgaccctg ccattttcca ccttgcggta
     6781 aggcgtttca atgaatccga attcattgat gcgggcgtag gtacacatgg agttgatcag
     6841 gccgatgttg ggaccttccg gcgtttcaat ggggcaaatg cgcccgtagt gggaggggtg
     6901 tacgtcccgg acttcgaatc cggcccggtc acggttcagg ccgccgggcc ccagggcgga
     6961 aagacggcgc ttgtgcgtca attccgcgag ggggttgatc tggtccatga attgggagag
     7021 ctgggagcgg ccgaagaagt cgcgcacgac ggcgctgagg gccttcggat tgatgagttt
     7081 gctgggcgtg acgccttcaa tgttctggtc aatgagggtc atgcgttcct tgaccaagcg
     7141 ctcggtgcgg gcgaggccta cgcggcattg attggccatg agttcgccta cggcacgtac
     7201 gcggcggctg cccaggtggt cgatgtcgtc caccatgcct tcgcccttct tgaggcgcag
     7261 gaggtatttg agggcggcca ggaaatcttc cgccgtcatc aggcgctggt ccaggctggt
     7321 atccagcccg agtttctggt tgattttgtn nnnnnnnnng ccccacgcgg gtgaggtcgt
     7381 atttcttggg atcgtcaaaa agcctcttaa gcagggtgcg tgcctgagcg gcagtagcgg
     7441 gatctccggg acgcaggcgt ttgtagattt ccttgagggc ggattcttcg tcgtgagcgg
     7501 gatccttctt cagggttttg atcagcacgt cgtcctcgct ctggtcaatg acgtcgattt
     7561 tcttgatgcc gaactggagg agctggcgaa ccacacccat attgagctgt tcgaaagcct
     7621 tggcaagaac caggtccttg tccttgatcg tgtccacagc cacaaggttg tgcagatctt
     7681 cttccgtcat gtcttcgctc aggggaagcg tgcggatgtt gtagaactgg ctgacgatgt
     7741 cacggtccgt tttgaagccc aggtagcgca taaacgtcgt cgcaaggaat ttgcggcggc
     7801 ggcggcggcg gtcaaggtaa acgtaaagca ggtcgttggt gtcaaactgg acctccagcc
     7861 aggagccccg gtccgggatg atgcggaagg agtggagcag ttttccgttc agatgctggg
     7921 cgctttcaaa gcaaatgccc ggggaacggt gcagctggga cacaatgacg cgttcggcgc
     7981 cgttgatgac aaaggtaccg cggttggtca tcatggggag ttctcccata tagaccgttt
     8041 cttccttgga ctcgttgtcg gatttgagct tgaaggtgac ctgaagggcg gcgctgtagg
     8101 tttcccccgc gcggatggcc tcgtaatcgc tcatcttcgg ctgttcaatg tcataggaga
     8161 cgaagtcgag ctcaatgttt tcgtcatagc tcttgatagg aaagatttcc ttcagaacgc
     8221 cttgtaaccc gatgtttttc ctggccgcgg ccggcgtgtc ctgttgtaga aaatcgaagt
     8281 atgattgaag ttgaatctca ataaggttcg gaggttcgat gacttccttg atattcccga
     8341 agtagagtcg ctttgacatg ggctgctgtg gcggagatgt tatggtttgc atcctgaaaa
     8401 acccggatgc cgggttgggc ggttctttcc ctgggggacg gaaccgactg gaagcgtttt
     8461 acgaaataaa aggcctctag tcggtcacgc gggatgtgaa gacgaggaag cgcaacagga
     8521 gaaaaacggc ttttctcctg ttgcgcggga agtaaaaagt ttacttgacg tctaccttgg
     8581 cgccagcttc ttccagcttg gccttggcgg cgttagcttc gtccttggag acagcttcca
     8641 ggatgacggc gggagtgcct tcaaccagtt tcttggcgtc caccaggccg aggccggcct
     8701 tcacttcgcg gacggcttta attacggcga tcttgttggc gccggcttcc gtcaggacga
     8761 cgttgaattc ggtcttttct tcttcagctt cggcaggagc ggcggcagcg ccggcggcag
     8821 ctacgggagc agcggcggaa acgccccact tttcttccag aagcttgacg aggtcggcag
     8881 cttccaagat ggtaagggtg ccgagttctt cagcgatttt gttaatatca ggcattgtat
     8941 tgttttctag tttggtttcg agtcgcgcca agagccaact tggcatttca gttctccggc
     9001 cggagttccg acaaggaacc tgtaggagag tcgggtccgg ttttcacaca tttggctaga
     9061 gtgcaaccgg accgtccggt caaggggttg gtattaggcg gcgggttctt cgctgccgcc
     9121 gttttctttg tccacatatg cctgaataac tcgggcaaga gcggaaccgg ccgcgttgat
     9181 agtgccaagg agtttggcaa gcaactggtc gcggggaggc agttcgccga tggcgcggat
     9241 ttcgtccgcg gaaagaatgg ctccatccag gatcgccacc ttgtattcgg ctttcttgga
     9301 agccttggca aaggtgttga tggccttggc ggcgcctgct acgtcggatg cgccggtcac
     9361 gaaagcggtc tgaccgacga ggtgttcccc gatgtcggga aggccggcat ccgtcagagc
     9421 cgtgcgcatg aagttgttct tggctacgtg gcactgagcg ccgcaggcgg ccagggcgga
     9481 acgcagattg gtgaactcag gcacggtgat cgaggtgtag tccacgacga tcaggaacgg
     9541 ggaggcgttg acgcgagcgg tcaggtcgtc aatgatgata cgcttgtcag gattcatggt
     9601 gtgctaaatc ggttcgggtt acagtttggc gatggaagcg gtgtcaaccg gcagaccggg
     9661 gcacatggcg catgaaacgg tgacggattc gatgtaggcg cccttggcgg aagccgggcg
     9721 ggccttgacg acggaatcaa tcagggcagc gatattttcg gcgatgcttt cgttggagaa
     9781 agacagcttg cctacagcgg cggagatatt ggcgttcttg tcaaccttgt agtcaacgcg
     9841 gccggccttt acttccttca cagccttggc tgtatcgtcc gtgacggtac cggttttcgg
     9901 gttgggcatc aggccgcgcg gacccagtac acgggcaact ttgcgaacct ccgtcatggc
     9961 gtcgggggtg gcaatggccg tatcaaagtc aacaaatcct ccctggactt tcttgatgag
    10021 atcttcaaac cccacgaatt cggctccggc ttcctgggcg gccttggcgg catcgccctg
    10081 agcgaagacg aggacgcgga cgtttttacc cgtgccgtta ggcagggaaa cgctgccgcg
    10141 aaccatctgg tcggatttgc gggggtccac agtcaggtgg aaggagacgg tgacggtcgg
    10201 gtcgaatttc ggtgccggga aggatttcat ggtttccacg gcttccgtga tgctgtagtt
    10261 cttgttaggg ataacaagct tggctgcttc ttgatatcgc ttgctgcgtt tggtagacat
    10321 gtgtgnnnnn nnnnnatgtg tgtatttggc agtgcgttcg gactggttta atcctcctgc
    10381 ggtgagtgtt acataccttc aacttcaatg cccatctggc gagcctggcc ggcaagaatg
    10441 cgggcggcgc gttcagggtc tttcgtgttg aggtcaggga gtttggtgtt gacaacttcc
    10501 atcagcttgg ccttggaaag agtggcgact ttctttttgt tcggttcgcc ggagccggag
    10561 gcgatgcctg cggctttctt gagaagtacg ctgcccgggg gctgcttggt gatgaaagaa
    10621 aatgacttgt ccttgtaaac cgtgatgacg gtcggcagga gatcgccggc ctgcttttgc
    10681 gtggcggcgt tgaactcctt gcagaatgcc atgatgttta caccggcctg accaagtgcc
    10741 ggacccacgg gcggggaggg gttagcggct ccggcattga tctggagctt gatgattttg
    10801 gttatttctt ttgccatggt tttgctttgt tagcggagaa aataaacggg aagggggctg
    10861 atggggggag acgcaaagaa gattcaggct ttttccacct gccagtattc caggtccaca
    10921 ggcgtggcac ggccaaaaat ggagacgctg acacgaagct tgcccttttc cgggtcgatt
    10981 tcttcgatgg tgccttcctg gctctggaac gggccttcgc acacgcgcac agggtcgccg
    11041 acggcgaatt ccaccttggg acgagcggca acggcttccg catccaacag gggcagaagg
    11101 tcttccacct cgcgcttgcg catggggagg ggggcgttgc gggagccggc aatgctgatg
    11161 acaccgtcaa tttgctggat aaagtaccaa agatccttgt tcagggtgcc gtccgcttcc
    11221 agcaggtaca tgttggcata aacatacccg ggaaagagtt tgctgtgacg ttccgttttt
    11281 ttgccgttgc gcacttcgga aataagttcc gtggggactt ccacccggta aatgaagtcg
    11341 gaaagctcgg cgtccttcgc cttgcggatg atgcggtcac gaacgctgtt ttcctgtccg
    11401 gagaggacgt gaaggacata ccattgttcg tgaggctctg gggtactggg catgatcgga
    11461 gaaagaagtg gggagagtaa aactagattg ccaactggat gagatagttg accacgctgt
    11521 gaagaaaaat gtcgaacgat gcaacaaacg cgccaaggaa taccatggca atcagtacga
    11581 cgagagtgga accccagagt tcgcggaatt ttttgaaacc tttcaccttg gggtcgcttt
    11641 cccaaggcca ggtcgtcttc ttaagttcgc ccttaacttc ggcgatgaat tgagaaatct
    11701 tgcgaaacat gcttcaaggt ggggtggtgt atttggaaag aagtggcagg tcaggaggga
    11761 ctcgaacccc caacttttgg ttttggagac caacgctcta ccaattgaac tactgacctg
    11821 ttggagtctc ttctttcgtg ggaaatgagg gagcctgcgg ggaggccccc tcattaaacc
    11881 tgatgtagat tcctctacaa gttcctgagg aacttagtca aggatttcgc tgatacgacc
    11941 agcacccacg gtgcggccgc cttcgcggat agcgaatcgc atggcttttt ccatggcgat
    12001 cggagtgatg agctgaactt ccaggttgac gttgtcgccg ggcatcacca tttccacgcc
    12061 ttcgggaagg gtgcagcaac cggtcacgtc cgttgtacgg aagtagaact gcgggcggta
    12121 gttgttgaag aacggagtgt gacggccgcc ttcttccttg gtcaggacgt aaacttcagc
    12181 cttgaagttc ttgtggggct tcacggtgcc gggcttgatg attacctggc cgcgttcgat
    12241 gtcttccttc ttcaggccgc ggaggagcag accaacgttg tcgccggcct ggccttcgtc
    12301 aagcagctta cggaacattt cgatgtccgt aacggcggtt ttctgggtgt ccttgatgcc
    12361 gatgatttcc acttcttcca tcttcttgat gacgccgcgt tcgatacggc cggtagccac
    12421 ggtgccgcgg ccggagatgg agaatacgtc ttccacaggc atgaggaagg gctggtccac
    12481 aggacgttcg ggctgcggga tgtaggagtc aacggcggcc ataagttcca tgatggaatc
    12541 ttcggcagcg gcgtcgcctt ccagagcctt aacggcggaa cccttgataa tgggggtgtc
    12601 gtcgcccggg aattcgtatt cgttgagaag ttcgcggatt tccatttcca cgagttcaag
    12661 caggtcggga tcgtcaacca ggtcgcactt gttcatgtaa acgacaatgg cgggaacgcc
    12721 cacctggcgg gcaaggagga tgtgttcgcg ggtctgcggc atcgggccgt cggaagcggc
    12781 aaccacaagg atggcgccgt ccatctgggc agcgccggtg atcatgtttt tcacatagtc
    12841 ggcgtgtccg gggcagtcaa cgtgggcata gtgacggttt tccgtttcgt attcaacgtg
    12901 agccgtggag atggtgatgc cgcgttcgcg ttcttcgggg gcggcgtcaa tctggtcata
    12961 gccgcgtgct tcggcgaaac ccttcttagc gagcactgaa gtaatggcgg cggtaaggga
    13021 ggttttgccg tggtcaacgt gaccgatggt gcccacgttc acgtggggct tgttgcgctg
    13081 gaattgctct ttagccataa tattatgttt aggtttgggc tgctatgggt ctagcttgaa
    13141 gatggagctt ctggcgggaa ttgaacccgc gacctcatcc ttaccaagga tgcgctctac
    13201 ccctgagcta cagaagcata gacttcttcc gcctttcgct gctcctagag aagtcgtaaa
    13261 tccgcccaaa cctgcggttc tagcggataa atagcatcct cccgtgttgc ctgacgaaaa
    13321 gcggacgcta tgttacgttt ttggatgcag gatgtcaatg aaaacatgaa cattttttca
    13381 gctttatgcc acaaatacag agggtgcatg tataatatat ttatcaggaa tgtgttgaat
    13441 gtttggatct gtttaaatcc tccgtcaatt taagattggg gtcaacgtct tcatccagag
    13501 agtggaacag tcctctgcgg gctttgaggc aggtgacata agcgatcatg gcggcattat
    13561 ccgtcgtcag gtcgaagtcc gggagaacca gttccacttt ttcacggtca caggcgattt
    13621 tcaggcggga acgcaactcc ctgttgcagg agacgccccc ggagatggaa agggtgcggt
    13681 gaccggaggc gcgcaaggct ttcagcgcct tgtgaatcag gacgtccgtc acggcccgct
    13741 ggaaagaagc gcagaggtcg cgcaaagttt gccggggcag gccgtgagga tcgccgtttt
    13801 tcgtaatttt gggcagtgta tagagaacgg cagttttcag gccggagaaa gatacgttgg
    13861 ctgtatgctc tttcatcagg gcccggggga aagaaaaggc ttccggatcg ccttctgccg
    13921 ccaaccggtc gatttccggc cctccgggat agggaaggcc tagcattttg cctaccttgt
    13981 caaaggcttc ccctgctgcg tcgtccagag agcgacccag caggcggtaa ttccctacgc
    14041 cgcgcacatc cacaaaaagg gtgtgacctc cggaaacgac catgcccaga tggggaacgg
    14101 gaccgccggg gcgtttgagg aagggggaaa gcagatggcc ttccagatga ttgacggaga
    14161 cgaagggcct gcccgccgcc agagccagag ccttggccgt gctgttgcct accagaagcg
    14221 cagctaccag accggggcct cccgtagcgc cgaagacgtc aatgtccgca ggagctgttc
    14281 cggcttcgcg gcacgcggtc cggatgattc cgggaagatc cgctgaatgg ttgcgggaag
    14341 ccagttccgg cactacgccg ccgtgctggc ggtgaatggc aatttgggag gagatgacgg
    14401 aggagagtat ttccggagct ttttcctctc cggcagaacg caggatggcg actgccgttt
    14461 catcacagga ggattctatt cccagaacgg taagggattc cggcatggag gaagaacagg
    14521 gaggggaggt taggatgcct ggtggatttg ggccggcttg ttgccggtaa ccgtttccct
    14581 ggtaatggta acggtgtccg catttttcat gctgggcact ttgtacatga catccagcat
    14641 gagggtttca aagatggaac gcagagcgcg ggctcccgtg ccgcgtttca tggcttcggc
    14701 ggccatggct ttcagggcgt ccggcagcac gtccagtctg gcgccgtaca tggcgagcag
    14761 tttggaatat tgcttgacca gggcattttt gggttccgta agaaggcgga cgagctggct
    14821 ttcatccagc ttggagagcg gacagaagat gggcaggcgt cccacgaatt ccggaatcat
    14881 gccgaaggag aagaggtctt ccggcatggc ctgtgccagt atttcctctt ccgagtattc
    14941 cttgcggtcg cgttgttccg tgatggctcc gaatcccatc tgggtggcgc cgaggcgttt
    15001 gcggatgatg tcttccagcc cgacgaaagc gccgcccaca atgaagagga ttttttccgt
    15061 attgacgcgg atgtattcct gttgcgggtg cttgcggcct ccggtgggag gaacattgca
    15121 gatggtgcct tcaatgattt tcagcagagc ctgctgcacg ccttccccgg agacgtctct
    15181 cgtgacggag acattctgtg ttttgcgtcc gattttgtcg atttcatcca cgtagatgat
    15241 gccttgttcc gctttcgcta cgtcaaagtt ggcagcctgg agcaggcgca ggatgatgtt
    15301 ttccacatct tcccctacgt aaccggcttc cgtcagcgtg gtggcgtcca caatgcagaa
    15361 tgggacgttg agcattttcg ccaacgtttt cgccagcagg gtttttcctg agccggtggg
    15421 gccggccagc aggatgttgg atttttcaat ttccacatcg tccagggact tgtcgtccag
    15481 catgacagca ctttggcgca ggcgcatgta gtggttgtac acggctacgg aaagcacttt
    15541 tttggcgtag tcctgaccga tgacatactg gttgagcgta gcgcacattt cttccggtgt
    15601 gggcagcggg ccttcatagg aggccgggcc ggccgccgtt tccctgagca tgtccgcggc
    15661 tgccgctttg cgggcctccg cctggcacat ggcgtaagcg gagggttcca tgttcaggat
    15721 gccgcggatg aagcgggttg ccatcggaag gcctgtgtcc ttcacaatca tgttgacgca
    15781 aatttcaacg caattgttgc agatgtaaaa gtcttccgca atctggatga gtttgtccac
    15841 cttgttcccg ggcttgccgc agcaggaaca ggcaggaaga tgaggggtgg aaccggacat
    15901 ggtttttcag gtagagagag aaatggcgga gggaaagggt caggccttgg tttttttctt
    15961 gtcgccctgg tcatcagagt gatgcgccag aactttatcc accaggccgt aggccacggc
    16021 ttcctcggca gtcatgtagt tgtcgcggtc agcatccttt tcaatttgct tttccgtttt
    16081 accggtatgt ttggccagaa tgccgttgag gcgttttttg aggttgaaca tgaagccgat
    16141 ggtgcgttcc acatctgaag cggttccctg ggcacctccg gaaacctggt gaatcattac
    16201 gtgggagttc ggcaggcaga agcgtttgcc gggagttccc gcagccagaa ggacggaacc
    16261 catggaggcc gctatgccca ggcaatatgt atttacgtca caagagacga attgcatcgt
    16321 atcgtaaatc gcgagacccg ccgtgacgga tcctccgggg gagttgatgt agacgtggat
    16381 gtcttttttc gggtcagcca tctgaaggaa cagaagctgg gcaatgacgg aattcgccac
    16441 atcgtcgtca atagcggagc cgatgaagat aacgcggtct ttcaggagac gggagtaaat
    16501 gtcaaaggca cgttcgccgc ggccgtcctg ctccactacc atgggcacat agtaggagtt
    16561 tgcgggggtt gatttggaag attggtcttg aaacatgttt atggaagatt ggatgttgtt
    16621 ttttcaatgt acgttacccg tccgaaagat gcaaaacggg ggagtcgggc atgtgtgcgc
    16681 caactccccc tggtaaggtg ttttgtgcag aggaggggtt actgctcgtc taccgttact
    16741 ttggcctctt ttgtcaggaa gtccagaacc ttggctgtca gcaggctcat tctgatgcca
    16801 tgcacgcggc cttcgcgctg gagttcgcga atgtaggatt tgaggttctt tttctgctgg
    16861 cgggcctggc gggccacttc gttgtaaagc tccatttccg tgacggcaat tttttccacc
    16921 tgcgccactt cctgaagcat gaagaagacc ttaaggttgc gcttggcctc ctgtttggct
    16981 tcttcccggg cttcctccac aaacttatcc atgtccgccg gggcgtttcc gctgtacatg
    17041 gcctgctgca ttttctgctg gaggatgccg tacacttcac gttctacgac agcttccgga
    17101 aggttgaagt caagcatgtc cgccagcttt tccgtgatct gatcggcctt ggcctcatca
    17161 atttgcatgg ctttgcgctg ggccaggttt tccttcaggg cggttttcaa ttcttccagg
    17221 tttttgccgg gaagaacttt ttccgcaaaa gcatcatcca tggccggaag ctgtttttcc
    17281 cgtacttctt tcacagtggc atggaatacg agttccttgc cgcgcagttc cgtaattggg
    17341 aaggcatccg gaatggtgac ggtgatatcc ttgctgtcac cggcattcag gctttccagg
    17401 gcggaggcga agccgggcag gaattgatcg tcttccacct tcatccactg gccgtcacgg
    17461 ccttccagga agcctacggg tttgcctacg gcttccgcta cgggcttgcc gtccaacgtg
    17521 gttttgaagt cgataatcgc cacgtcgtct ttttgggcgg cgcggtccac aggggtgaat
    17581 tccgcgattt gttcagccag ggagttcaga gcttcctcta cgtctgcttc cgttacttcg
    17641 gaagagggaa ccttgacttc gatgcctttg tattccggca attcaaattc gggaaccacg
    17701 gtcatcgtgc tggtagcggt gtagttgccc tggtcatcca gagattgttc cggctttccg
    17761 aagttgagca ctttcaattt ggggttttct tccagagcgg tggagcaggc cgtttcaaaa
    17821 agggtgtcca gcagttcttc ttcaatttct ttcttgaagc gcttttcaat gatggacttg
    17881 ggtgttttgc cggggcggaa gccgggaagt ttggctttgg cggcatagga atctacaatg
    17941 gaggcgcggc gcgcagctgt ggtttctgca gggatggaag ctttcagcgt ggctgtgcag
    18001 tccggttgaa tatcaacgtt gatttccata aataatacgg tcagtgttaa ttgaaagtgg
    18061 ttctgtttca gctccgggat ggcggcagcg gtcaaaacac gagcggcgat aactttaatg
    18121 agagggtatc tggagtgcaa gctattgcac aggaatgaca caattgccct cctaagcgcg
    18181 cacgcgcacg cgatgtaatg gaatacgggg caactgtgtt ctacgcggaa tcacgtcttg
    18241 actatgcaag aggaaaagtc ctttatatcc caagcattaa gttcagcgct catgattcgt
    18301 cccgtttttt tgtgtgtttc ctttcccttg atagcatgtt cggctgtttt ggcccaggat
    18361 gaaacttccc ttaacagagg cgcctatggt tccgtgatga ctccggaaca ggtaatgcgg
    18421 gaactggggc cgggcacggg gcttcttccc ggtgaccagg cgtatgaggg gcggcctgtg
    18481 aagagcgttt ccgtgcggta cagaagcacc ggcaagaccg tgtccgaaga ccgtctgaaa
    18541 aatttgctgg ctacgcagcc gggcaccagt tattccccgg aagtggtcaa caaggacttg
    18601 gaacggttgt tggaaagcgg tctggtaggc ggcaatacaa cagtagccgt ggagccttcc
    18661 ggcgacggcg tggcggtggt gtttgaaatg gcggcccaga atctgctggg cggcgtgggg
    18721 ttccgcggca ataccgcgtt cgacaacagg gacctctccg aggaatgcgg gctgaagggg
    18781 ggggaagctc tcagcgacaa ggccctgagc agcgctattg ccaagctgcg cacttattac
    18841 caggaagccc ggtatccgga tgtacgtgtt tcctattttt accagaaaac ggaacggccc
    18901 ggctttgtgg atgtggtctt cgacattaac gaaggaaaaa aggccaatat tatcaagatt
    18961 gactttgtgg gcaatgaaca catcagcgcc aaggatctcc gacaggtgat gaaaaccaag
    19021 gagaagggct ggttgacctg gattaccaaa tccggacgca ttgaccgtga acaagtggag
    19081 gacgatctgg cggaattggt gacttattac cgcaataaag gttatctgcg cgtcaagctg
    19141 gacaaggtgg aatattttga cgccggcaaa ggcaatgagc agaagctgac gatgagaatt
    19201 acccttaccg aggggcgccg ctacaaggtc aaccaggttg ccttcggccc caccaaggtg
    19261 tttacggccc aggaactgat tcctggcctg agcatgtaca acggggatac ctattccgcc
    19321 cagaaagtgg ccgacgacgt gacgatgatc cgccgttatt acggttcccg ggggtatgcg
    19381 gacgcctccg tgcgtccgga cattcaggaa gtgggcgttg atcccaagac aggttacgga
    19441 caaatcaaca tcgtgtacca tgtgacggaa ggcaatccct accgcgtagg caatatccgc
    19501 ctgaccggga ataaccgcac gaaggattac gtcatccgcc aggaacttcc cctccagtcc
    19561 aatgacccca tgaacgccgt ggatttggat accgcccaga agcgcctcca gaacctgaac
    19621 tattttgaca tggtggacgt agcccaggtg ggatccacgc ggcccggcta ccgtgatatc
    19681 aacatcgagg tggcggaaaa acgtacgggg tccctgaata tcggcgtggc attttcctct
    19741 attgaaagcg tttacctgtt cgctggcatc acccagtcca attttgacat gtatgactgg
    19801 tcctctttcg tgggcggcgg ccagcgtttc gcgattaacg cccgtgtcgg ttttgaaacg
    19861 caggatgcca gtatttcctg ggtggatccg tggttcctgc accgcaagct ggccttcggg
    19921 acggaaattt tctacagcaa ttccacatat ttttccgatt attacgacca gcagaactat
    19981 ggctttgcca tttctctccg caagccgatc ggggaactgg attacgtgaa actggaatac
    20041 cgcctggaac agtatcgtat tgacgccgaa gggaacgccc ctatcttctt ttggcaacag
    20101 gacggagact acctgcgcag ccatgtggaa ctttcctaca ccattgatac ccgcgacgcg
    20161 cagattttcc cccgcaaggg aggcaaattt gaagtgctgg ccggttattc cggcctgggc
    20221 ggcgatgtgc atacatacaa tttcggcgtg aatggctcct attactggaa tttgcgcgga
    20281 gacaccattt tcagcatcaa tgccggtgcg gctaccgtgg attcctatgg caaccacgat
    20341 gtgcccattt ttgagcgtct ctacctcggc gggccataca acatgcgcgg tttccgtttc
    20401 cgggacgtgg cgccgtacaa tcccgccctg agcggtgatg agacgatggg cggccgttcc
    20461 tccttcttct gccagtttga atattccatt cctgtgattg aagaagtgcg cgtcgcggtg
    20521 ttttatgaca tcggttttgt caacggagat tcttttgact tcagcacgtc caaaatagtt
    20581 tccgactacg ggatcggcct gcgcctgaac ctgcccatcg gtccgctggc agtggactat
    20641 gccattccga ttcagaagaa caacgccatt gaccgcggag gacaattcca gttctatctc
    20701 aattattcct attaattacg tttgataatt tctctaccgt ttgacgacat gagtaatttc
    20761 cgcattctta tcctggcgtt tctggtggtg gtgctggctg ttctgggaat gatttatgtg
    20821 aatatttctt caagaatgga tgtgattcag caacagcagc agatgacttc cgctccggca
    20881 cctgtcaatc cctccccgta tcttccgatg cccacgcagg ctcctgcttc cgccgttcag
    20941 ccggtggtgg tggccgatcc tgtagtgagc gaggaactgg ctcatgtttc ggcggagctg
    21001 gaacggatga agaaggagaa tgaagaactg aagaagcgcc atgctctggc ggaggaagaa
    21061 cgcgatttgt tggaacgcca gaatatggaa aagggagatc cccggctgca atggcttaat
    21121 gaaatcaagg atgcggagca ggtgggccgt gtgacggaat actaccgtga cgccaacctg
    21181 gtcctgttcc agtccatcgg ccagcctgac ttgaaagtgg ggcaggaact gggaatccgc
    21241 agacgcggga gcattttcgt atccctgatt atcgacggag ttgacccgga cgggaaggtg
    21301 ttccagtcct acgtgaagcg taatacgctt tttgataata atgacaagga acccatcaag
    21361 ccgggggatg aagtcattgt tccgcctgcg agctggcagg aaaacatgac ggaagaagat
    21421 aagtccggta tggcttctcc ctccgccccc gtccccgtgg aagctcccaa accggtgatg
    21481 attcccatgg agccgtgatg cgggtccgta tttagccaag ccaagtaaag agaagtgcac
    21541 ctgagagaaa gattgcaatc gggcgccggg cccagcctgt cctttgaatt tttcccgccc
    21601 aaaaacgagc aggcagcgtc ggccttgtac cagactatcc gtgaactgga gccttaccgc
    21661 cccagtttcg tcagcgtgac gtatggagca gggggctcca cccgcgagct tacccgcgaa
    21721 gtggtgttgc gcatccaaca tgaatcccgt attcctacgg taccccacct tacttgcgta
    21781 gggcatagcc gggaggaggt atggaatatc ctggaccaat acgcccgggc tggagtgcgg
    21841 gccgttcttg cacttcgggg agatcctccc cggggagcga aggattacaa ccgcgatgcg
    21901 gatgtttttc gccatgcggc ggatctggtg acgtttatcc gtgaatataa tgaatccggc
    21961 cgtcacccgg atccggatgg attcgccata ggcgtggccg gcttcccgga aggtcatccg
    22021 gccactccca accgcctgcg cgagatggat ttcctgaaag ccaaggtgga tgccggcgcg
    22081 gattatatct gcactcagct gttttttgac aatcattctt ttctggatta ccgggaacgc
    22141 tgtctgctgg ccggcatcaa ggtgcctatc ctggcgggaa ttatgcctgt gaccagcctg
    22201 tccggcatga accgcatggc agagctttcc ggaggcacct gttttccggc caggctgctc
    22261 aaggcgctga agcgtgcggg ttccaatccg gacgctattc aggaagtagg catccaatac
    22321 gcctccagcc agtgcgccga actgttggat tccgatgtgg acggcattca tttttatacg
    22381 cttaaccaga gccgggcaac gcgggagatc tacaggaatc tgggactgaa ggattccctg
    22441 cagcttttgg aacatggcga agataagtaa aaagctgatt gatttggttg ggacaacacg
    22501 gtagaaacct cttcaacata ttatggatat caaggatatt aaacgggcca tggacaatat
    22561 gccgatgggc gacggaagtg cgtttgcgga aatttccgtc ggcccggcca agcatgaaca
    22621 gtttttggat gaacattacg gcaaggttgc gctggttgtg ttgctatgcg tccttggggc
    22681 tgccggatgg attatttata atggcgtgca ggattccatg gaaagaaagg caggcgccgc
    22741 tctggtggcc gctatgccgg aatccccggc aaccggggaa atttccctgg atgaacaggg
    22801 gcttcagcag gtagtggctg actttgacag ttcccgcgcc gccgtcaccg cctcttacct
    22861 tcaggccatt gccttgtgga acgccggcaa ggaggatgaa ggcgtggcca aaatgaaagc
    22921 ttttattgat tccgctccca cggaggaatg gaaagcccag gcttccgtta ccctggcctg
    22981 ccgtctgatg aaccggggga aagcggatga ggcggaaggg ctgttccgtt ccgtggtgga
    23041 tgcgggagat cctacttttt ccggttttgc caccatgtgc ctgggggaca ttgcccgggc
    23101 taagaaagac aatgccgccg caggcgcctt ttaccgtgaa ctggcggaga aatttccgtc
    23161 cagtgcattt gccttgcaga gcggaggcta cctgctgcgc aagagcctgt ttgatattca
    23221 gagtcccgtt cgtattgagc ccgcagtaga acagaaataa tccatgcggg cgtttccgaa
    23281 gttttccgga agatgaacgc tttttccatc cgcatgtctg tttttttaca gtgcatgcgg
    23341 atttccgtat tcaggaacgc cggaacaggg gggtaatgaa aagcctgctg tgcgcgtagc
    23401 tgttatgatt ccgggaattc cgttcaggac tgtgacatat ttaaacaaat catcaaggga
    23461 ttccgcgctt accggatgta accgggctgc cggtgttaat ccttattttt tatctcatga
    23521 acaggcatat taaatcattc tggcttcttc ccctggcggg aatgacgctt tctttcatgc
    23581 cgtcctgcac gacggacagc tacgggaaca gttccgtaac gcccggcgga gccgccgcca
    23641 tcggtgtggg ggcgctggct gccggcgccg ctattggggc ggctgtccag catgaccgcg
    23701 acaaggacag gtaccgcaat gactggcgcc accataatcc tcctcctccg cctccccgtc
    23761 cgcctcatcg accccaatgg cggaggtaag aagggagcgt gcgtgtaagg gcggcgatat
    23821 tttggttcgg cagggggccg ggaggcgacg cgtttttccg gtctgcccct ttgctgaaat
    23881 ctcagacccg ccgtatgccg cggagcgccc aggtcatgcg ttgtctggcg ctttccggat
    23941 cattgaggat agaacccagc aagtcgagaa cgggccgagc tgctttgggg tcgtgcactt
    24001 ggagctgtcc ggaaaggata aagggggctc ccccgcagca gacaaggtaa atgaggatgt
    24061 tcatggggtc tcccatgagc acggatcctt cctgctgggc ttcccgtatg gtttccagca
    24121 gcacaagggg atggcgcgta tggattcctg aaatgaaacg gatggccgcc tgttcccctg
    24181 ccagggcgtc cagaaccagc tgggcaatga attggccgtg acgcagcagg aactccatga
    24241 cgtctcccag gatggcttcc agtttttcaa aggccggaag atgaatgttg gcatgggtcc
    24301 tgatgccggt gaacagaggt tcgtaccagc gttccagaag ctcggtcaga aaagcttccc
    24361 ggttgccgaa gtgatagaca aagctccctg tattggtgcc ggcccgcagg gacaggccgc
    24421 gaacggtaag ggcgcgcagc ccggattccc cgacaatcat ggcacccgta ttgagcaggt
    24481 gctctctggt ggatttccgt ttgggcatga aggtgaagct tgaacctcca tgtgtgcccc
    24541 ttatcggcct gcaaggcaag gactcgttat gtctgttgct gtaaaaagcg gaaccggggc
    24601 tcatcctctc cccggttccg ccgccggagt cctgaatgaa tcaggaggtt gctttcaatt
    24661 ccttccgccg ggaggcaatc cagcgggaga agagtttggc cgtggccgga atgataagca
    24721 ggttcaggat ggtggctgtc accagcccgc cgaactggac gatggccagg gggccgagca
    24781 gttcgcctcc gggctggtca atggcccaga tcagcgggag cagccccagc acggtagtca
    24841 gggaagtcat gatgatgggg accacgcgtt ccagagaacc ctcccgtatg gcttccgcgg
    24901 gttccatccc ctgatgttcc agggcccggt acctgttgag cagaatcagg ccgctgcgga
    24961 tggcgaagcc gatgacggtc acgaatccca caatggaagc cacggaaaga atgggaggaa
    25021 tgtaagttcc tccaaacagg gaggacagcg taccggggga ggcaaggaag acggctacga
    25081 taccgcccac caggcacagc ggtatgttca ccagggtcag catggcgcgc cggacgcttc
    25141 ccagtgcgga ggaaaggagc agcacgatga gcaccatgac gatggcgccc aggacgtaaa
    25201 gccgttgtga tgcggattcc cgtgctttga tagtgccgtc atagtctacg gtacagccca
    25261 tggcgttcat taccgggtcc aattgttccc gacaggcttt cgcaaggtct cccaggttgg
    25321 aattggggga gggattgcag gaaatcatgg ctttccgcat ggtgttgtcc ctcaggatga
    25381 ggttggaaac ttcttcccgg tacacctgtg ccacgtcgtc caggcgcacg gtttttttat
    25441 tgggggaaat gagttccagg tttttgacat cttccatgga cgttttaagt ctggggtcaa
    25501 tgcgcaggac aatgttccaa tggtcctggt ttttgatgac ttctcccagt ttctgtccgt
    25561 tcatggccgt ggaaacctgt tccgcagcat tcgccatggt caggccgtag gaagccaggg
    25621 cttcctgatt gtattgcacg cgaatggtat ccaccataat ttcccggttg gcgcgtgcat
    25681 ccgccacttc cggcatgttc tccagaattt ccttggcttt ccgggccgcc aggcggagct
    25741 ggggcagctc cgtgccgtaa atgttgatgg cgatttccga gttggagccg gacagagcgg
    25801 aactgatgcg atgggccagc ggatagccga tcatggagct ggtgccgggg atgccgtcaa
    25861 tgcatttctt gatggcggcg cgcaattctt tctggtcttt cttcagatcc acgcgcacga
    25921 gcagctcgga ggcgctgacg ggttctgcgt gttcgtcgtt ttccgcgcgt cccgtgcgct
    25981 gggtgacact gagaaccccc ggaatctgtt caatatcttt catcactttc cgggagatgc
    26041 gctccgtttc atccagggaa gttcccggca ccgtgctgac gaagactgtg tagcaatcct
    26101 cgttgaacgg cggcaggaaa ctggttccga acgtggaacc cagccacagg gccagcagcg
    26161 taatggccgt catgatggcg cagaccgttt tggaaaagcg caggcagaat tccaggatgg
    26221 gggcataaat gcgtttaatg aggcgggagc tgaaagaatc cccgctttcc agagtagcgg
    26281 cgtttttgct tttcttgaac catatgtaac agagaaccgg agtaatggtg acagctacaa
    26341 taagagaaga aagcagggcc agcatgtagg agatccccag gggccggaaa aactggcctt
    26401 ccagaccgga caggaagagc accggagtga ataccagcag gatgatgacg gaggagaagg
    26461 aaatggagcc tacgatttcg ctcttggctt tcatcagaac ctcatacttg ctcttgcgct
    26521 tatctttcgg cagggcggcg ttcctgttca ggtgcctcca ggctatttcc acgaagataa
    26581 tggcgttgtc caccacgtct cccacggcta cggcaaggcc gcccagggtc atgatgttga
    26641 tggccagccc gaaggcgggg aacatcatca tcccgaacag gatggacagg ggcatggaaa
    26701 tcagcgtgat gatggccgtg cgaaggttca gcagcgtcag gaagatgacg atcatgacca
    26761 ccgctccggc aatgaggagg gtttccgtgc cgttatccag ggacatttca ataaaatccg
    26821 cctgccgata ggctgcggtg tgcagtttca ttcccttggg aagctggctt tgactgaatt
    26881 ccctgacggc tgcatccacg gcctgtgtca gggtcagagt attggctccc ggcaccttct
    26941 gcacggaaag cactacggcg tcttccccca taaatccggc atctccccgc cgcggcgcac
    27001 cgtctatctt gacttccgcc acatcctgga gccggagtat tccgg
//
LOCUS       DAWEMX010000017        26683 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_745_length_26683_cov_11.336826, whole genome shotgun sequence.
ACCESSION   DAWEMX010000017 DAWEMX010000000
VERSION     DAWEMX010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 26683)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26683)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26683
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_26683_cov_11.336826"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1076..1085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7961..7970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10580..10589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12485..12494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6486 a         7751 c         6734 g         5672 t
ORIGIN      
        1 gactctccca tgcccgtttt ccagtattcc cgttccgctt gcaaataaaa cgcaccgaac
       61 ggaggcagga tttttaacgt tttcccatgc cttgcggcca gttccggaga gggcatgtcc
      121 tcccgcatat gcttcccttt tccaatcagg aacggcagca ctaccacctt ttgccccttt
      181 aatccgggta atacctcttc cacagaagga aatgcgccaa agtatgcaag cgccatgtcc
      241 gttccttccg gaagccggaa tttcaactgc tggaggaatt gcgccggctc aggggcagcc
      301 tcccttcccg ttaccccgtg ggctaccacc agcagggaag cctccccctc caattccttt
      361 tgcaaggcgg cggccctaaa accaacaccg ccgcatcccg ctccccatac gggtttaaaa
      421 acaagttccg gatgctgccc gctcccggca tacagcgcac gaagcaattc cggcaatgtc
      481 tccgtcaccg tcacgccgct ctgcatgaag acagggtaaa cccagactac accatcgccc
      541 ggaggcaaat ccgccgccgt caggctgcgg aaagacccct gccagacaca gtcgcttgaa
      601 tattgaaact gctccgccac atgctgccgc aattcagccc ctgcttcatc ataggaaata
      661 aagagtttgt aaatcttcct gggcatttcg gtaagaccac tgcgggggaa cggcgttcgg
      721 aacatcctgt catttattca tgcggaagta acgcatgatg gccgtttgta acaaaaagtc
      781 cggagggcgg caactccata cgcttttttc caatccatgc tcccctccat ttacacgcac
      841 ctatctaaaa aatatttacc atatttataa caaaatcatt atatcgaata taaaaagata
      901 cttccggaaa aataaacctt cgcctccgaa tctccgcgcg gaggcaggat agcggcttga
      961 ccgaaaggcc tgaaaccgtc atgatacaag gacatacagg ttttgtttta tgtcgtttta
     1021 cattcaatcc gttgaaatcg gcggcccgcg gccgttctac cttctgggcc cctgcnnnnn
     1081 nnnnncccct gctctctgga atgcgaatcc tttgtatggg agatggcccg ctccatcaag
     1141 gccatcgcgg aaaacctgga tgtcccactg atcttcaaag cttcctacga caaggcgaac
     1201 cgcacttccg tcacctcttt tcgcggcccc ggcgtgcggg aaggctgccg cattctgtcg
     1261 gaaatcggca gggagctgaa actgcccgtc gttacggatg tgcatacggc ccaggaagcg
     1321 gaaattgccg ccgaatacgt ggatctgctc caggttcccg cttttttgtg ccgccagacg
     1381 gatcttctgg aagcctgcgc caagagcggc cggcccgtga atatcaaaaa agggcagttc
     1441 ctggctccgt gggataccgt gaacatcggg gaaaaaatgc gcgccttcgg ctgtgacgaa
     1501 ttcatgatgt gcgaacgcgg caccaccttt ggttataaca atctggtggt ggacatgcgc
     1561 tccctgtact ggatgaaaca ggaaggcttt cccgtcgtat tcgacgccac tcacgcagtt
     1621 cagaaacccg ggggattggg aggaaccacg ggaggagaca gcgagctggc ccccgtgctg
     1681 gcatccgccg ccatggcgac gggcagcatt gacggcgtgt tcatggaggt gcacaaagac
     1741 ccgtccaaag cgctttccga cggccccaac caaattcctc tccacctgct tgaaggagtg
     1801 ctgaaacgcc ttcagtccat ccaccaggcg gttcagtcat gatgaaatta ttgtccacat
     1861 gctttttgac ggccgccctg tacggcacgg aggcgcaagc ggacaccagc ctgcccgcag
     1921 ccacgccttt tgccggcgta gataaaaaat tacctatgct ggagcttctg cccgtaggcc
     1981 aggtgctccc aaaagtcagc ctgtcacgat atgaagggcc gcgcctttcc atgctgctga
     2041 cggcttcaag cgtgaccgtg gccgccccgc aggaaattgc cgcgaatatc ctgaacgtgt
     2101 acttgtacgg aaaaaacaac cagacctccc accttttcac gcaggccgcc tcttattttc
     2161 tggataaaaa actggccgtc tcccatacgg aaaccaccct cagggaagag aatctttccg
     2221 ccaaagggac cggcctttac ctggacacca atatcaagag gggcatcatt ctgggtcccg
     2281 ttcaaaccac catccgcatg gctcaacgca acaagtaacc attccttttt atgaaaaaac
     2341 tttcctccct gctgctggcc ctggccggaa gccttgcctg cagcggcgcc aacagcgttc
     2401 cctcccacat tgtccagccc ctgcccaggg aactggatcc ccgcctggaa caacccctca
     2461 ccccgccttc ccatattgta cgccccctga cggcggaaga gacagcccgg agggaacggg
     2521 aactggcccc tgttccgacc gcagacttga aggaagacgg ggaaaaggcg attcaaacat
     2581 acagcgcgca ggaaaaaacc gtagacgggc ggattcagga attccgccag cagcaccagg
     2641 ttgccggaaa tacccccgcc ccggctgcgg aaactactcc ctcttccgca gcatccggcc
     2701 cggaggaagg cttcctgcag gccaattccc caaaagacct gtacaccgta gattgtgacg
     2761 gagacttgta cttcgatatg gaagaaggcg ttctggtgtt catgaaaaat gtccgggtgc
     2821 gcaaccccgg cctttccatg gattgtgcgg atcatctgaa aatttatgcg gaattcgtgc
     2881 cgaagaaacc gggcgacaaa aaaccctccg acgtcaagaa ggaaaagggg aaagaagaaa
     2941 atcagactca ggtggctctt cccaacggag gcaatttcga ctacaattcc attaaaacgg
     3001 ttgcggcttc cggaaatgtg aaagccagtt acaccaacaa acagggagaa acaagcaacg
     3061 cccaggctga aaaaatcacc tacaacgcca aaaccggaga aatcatcctg acgggtgccc
     3121 cggtcatcgt cactccggac gtcatcgtga aaaatccgaa caaggatgct ttcattcggg
     3181 tgtattccaa cggaaacatg tacggcagtc gcggaaccaa gactacgttc cgccacgtag
     3241 acaaaaaact ttccgaccgg gaaaaccaga aaaccagccg caaaccgtga gttcgttcca
     3301 ctcttctcct ttgctaagcg ccgaaggcat ctgcaaaacc tacaaaacgc ggaccgttgt
     3361 ggaccaggtc agcctgaagg taggccatgg cgaaattgtg ggcctgctgg gaccgaacgg
     3421 cgccggaaaa accacttcct tttacatggt agcgggtctc gtacgcccgg atgaaggccg
     3481 cgtgattttt gcggacgaag atattacgga attgccgatg aacctgcgag ccaggcgcgg
     3541 catgggttat ctgccgcagg aagaatctat tttccgtaag ctttccgtgg aggataatct
     3601 tctggccatt cttcagaccc gcaaagacat gacttcccgc cagcaaaagg accgcgccgt
     3661 agagctgatg gaccgcttcg gcatcaccaa gctgcgccgc tccatggcca tccagctttc
     3721 cggcggcgaa aagagaaggc tgaccatcgc ccgcgccctg gctaccagcc ccaaactgct
     3781 gatgctggac gaaccgttca gcggcgtgga tcccatcgcc gtttcagaca ttcagaaaat
     3841 cgttatggaa ctgcgcgata cggacggcct ttccatcctg attacggacc ataatgtgcg
     3901 ggaaaccctc cacatcgtgg accgcgccta cattttgttt gaagggaagg tgatcaagca
     3961 cggtacttcc gaggaaatcg ccaacgaccc catcgcccgc aagcagtatc tgggcgacca
     4021 gttccgcatg taactttatt ttccgtcttt cccatgtcct ctccgcagaa tacgatcgcc
     4081 ctggttttcg actacgacca gaccctgagt cccttctaca tgcaggatca ggccatcttc
     4141 ccggaattcg gcatttctcc gcaggaattc tggaccaggt gcaacgccac cggagaaaga
     4201 gaaggctggg acggagaact gatttacatg aaaagcctgc tggacgccct ttccctggac
     4261 cgcgtcagca acgcccggct tacccagctt ggagagcgcc tcacctttta ccccggtttg
     4321 ccggactttt ttgacgcgtt cccgtccatg accctctcct cccttcatac ggatctgggc
     4381 atcagcattg aatactatat catctcctcc ggtttaaaag ccctgattga cggttccgcc
     4441 ctgcgccctt atttcaaggc cgtatttggg tgcgaattca gcgaggagaa cggctacatc
     4501 aattttccca agagagtcat ttcccacacc acgaaaaccc aatacctttt ccgcatcaac
     4561 aagggaatgc tggattacga ccaggacgta aacgaccaca tgccggaaga catgcgcccc
     4621 attcctttcg ccaacatgat ttacatcggc gacggcccta cggacgttcc ctgcttcacc
     4681 gtcatgcgcc ggaacggagg ccacgccgta gccgtatata atccggaaga tgaaaccggc
     4741 cgcagtttcc gcaaatgcta taacctggcc tccaattccg accgcgtgaa tttcatcgcc
     4801 ccggcggatt acacgcccgg cagccatctc acccgcctgc tgtccggcat ggtcacggat
     4861 atcgccaacg gcattgtgcg ccgccgggac cgggctctgg cggattcctg tattgcggct
     4921 ccttcctttt ccaaataaaa cccgctccat tcctctcccc gtcatgaaca gccatttcca
     4981 ctttctcggc atctgcggca ccgccatggg cgcggtagcc gccgccatgg cccgccgcgg
     5041 ctttaccgtc accggttccg acgccaacgt ctatcccccc atgtccactt ttctggagga
     5101 ggaaggcatc accctgtttg aaggctacaa ggcctccaac atcccgccgg atgcggacgt
     5161 catcgtcatc ggtaatgcca tgagccgcgg caatgaggaa gtggaagccg tgctccaacg
     5221 gcgcctgcgt tacctttccc tcccggagac gatgaaggaa tacttcctgc gcggcaagcg
     5281 caactatgtg gtaacaggca cccacggaaa aaccaccacc acatccatgc tggcgtggct
     5341 gatggaggat tccgggctga accccagttt catgattggc ggcattgccc gcaatctcgg
     5401 acgcgggggc cgttttacgg attccgattt ttccgtgctg gagggggacg agtacgacac
     5461 ggcgtttttc gacaagcgtt ccaaattcct gcactacctc ccggaactgg tggtcatcaa
     5521 caacattgaa tttgatcacg cggatattta caattccctg gaggaaatca aactgagctt
     5581 ccgccggctg acccagatca tcccccgcaa cggcctggcc ctggtcaacg cggacgatcc
     5641 caactgcctg gacgtggtta agggggcccc gtgccccgtc aagacggtgg gcttttccga
     5701 ctcttccaat atccgcatcc tggatgtgga gacctccacg gacggcagcc atttcacact
     5761 ggaaggaacc cggtactacg taccgatgat tggagagttc aacatccgca acgccgccat
     5821 ggcaattgca gccgcacaat ttgcgggact gaacgtcaag cagctggcgg attccatgag
     5881 ccgctttgaa ggagtggccc gccgccagga agtcaagggg gtggtcaacg gcatttccgt
     5941 agtggacgac ttcgcccacc acccaacagc catcaaacag gccatcctgg gcctgcgcca
     6001 gcgctatccg gaatcacgca tctgggccat tttcgaaccg cgctccaaca ccacacgcag
     6061 aaatatcttc cagaatgaac tggcgttgtc cctcgctacg gcggatttcc ctgtggtagc
     6121 cgccgtggat catccggaca aggtggctct ggaggaacgc ctggacctca ccaaactttc
     6181 caacgacctt tccgcgctgg gcaaggacgc tctgatcggc ggccatgccc aggaaatcgt
     6241 ctctgccgtc tgcaagaaag cccagccggg agacgttatt ctggtaatga gcaacggcgg
     6301 ttttgaaggc attcacgcca aattgctgga agcactgaac gcctgaccct tcttcataca
     6361 tccacttcca atgaaaaacg catccaccat caccgccttc atcatcagct tcctggtcgt
     6421 aggcagcctg gccctggttt tcctgttcct tgacaataag gaagaggaac ccgccgccgc
     6481 tgaaccggcg gcgcaaacgg aagtgccagc acgccagacg gtaacgcccc aacccaccgt
     6541 cgacccgctt accaaggaag atatcagccg gcagacggct acgccgccct ccctgatcaa
     6601 tctcagctcc ccccagacac tggcgcaggc aattgccaaa gtcattcagg accgggatga
     6661 ggacatcctg aaatccctgg aaatccagca ggccgtgacg gagaaccaga gccgcctcat
     6721 ccgggagctc atggaagccc gccatatacg cctttcctct cccggcatca ccagcatcgg
     6781 cgcgaacaac cagggggcca atcccacagc cagatacacg ctcaatttca gcagcggcgc
     6841 gcgcggctac ctggatatga agcgcaatga caagcaacag tggacgctgg ataccctcac
     6901 tctgcccagc aagcaggatc tggccaagga caaagtcgcc cccatggcga tgaatgaccc
     6961 catgggcatc gtcagttcct tcatggatgc cgtcgccaag gcggatttcc gcggggcgag
     7021 gaagttcgtg gacggaacta aagtgcagga tgccacggta gccggtttgt gcattctgtt
     7081 tgaggagggg gctttccggc tccgggagga cgctcccatc aaaaccgcct acgaagcgcc
     7141 caccaatgcg gggtttttcg ttcatcttca ggacgcccag ggcaggaagg ccggcaatgt
     7201 aggcctgacc gtggccaaaa cggacggaca atggctggtt gcggaagctt ccctggacag
     7261 catgctggaa gcctatacga aacggcaggg ggccggagac gacatcttta tccccattgt
     7321 aaaaaatccc cagggcggcg attccctggc cctgttcttc ggctttaatg aagataccct
     7381 ttccaagcgt tccgaacgcc agctgcaaat cgtagcggaa gctatcaaga tggacagtgg
     7441 caagaagctg gaaatcagcg gccatacgga tgacgtgggg agcgaacgct acaaccaggg
     7501 gctctccgaa cgccgtgcgg ccgccgtgaa agcccagctc gtccagttcg gcgttcctgc
     7561 ggaaagaatc gttaccaagg gattcggcaa atctcagccg cgccgaacct actcccccac
     7621 ggcagacgaa gaaacaaggg atgaagcccg caaggaaaac cgccgcgcgg aaatgtacct
     7681 ggacttttaa aagagtcatc agagagcaga gaagggacaa gcctttccaa cttctctatt
     7741 tactgttgcc tgtcaattat taaccatatt ccatggctcg ccgatatgtc atcaggcagg
     7801 atgccgcgcc ggtcgccccc atctccggga tcgactaccg tacggccctc aatgaagaac
     7861 agtacgccgc cgtttcttca gaaccggggc cggctcttgt catcgcagga gccggatcgg
     7921 gaaaaacgcg cacgctgact tatcgcgtgg cctggcttct nnnnnnnnnn acggtacgga
     7981 cccatggaac attctgctgc ttaccttcac caataaggcg gcgcgggaaa tgacggaacg
     8041 ggtgcgttcc ctgattcccc tggacttgtc ccgcctgtgg agcggcacgt tccattccat
     8101 tgccaaccgc atcctgcgcc agcatgcgga atacctggga tatacaccgg cttttacaat
     8161 catggactcc gacgaccgca aatccatgat taaaagcgtg gtcaaatccc tcaaactgga
     8221 tgagaaatcc acccgcttcc ccaagccgga agttctttca tccctgttca gtctggcaga
     8281 caacgaggga gccaatatcc gggaaacgca ggaaaacgcc tatccctacc tttccaacca
     8341 cctggacgcc atcctggagg tgcatgacca atacgtagcc cgcaagcagg aaaccaacag
     8401 catggacttt gacgacctgc ttctcaacgt cgtgaggctt tttcaggaac aggagcacct
     8461 tcgtgccctg tacggttccc gcttccagca tattctggtg gatgagtacc aggacaccaa
     8521 ttacctccag agccgcctca tcgacatgct ggcccgggaa cacggccagc tgatggtggt
     8581 gggggacgat gcccagagca tctactcctg gcgcggagcg gacatggaaa acatcctcag
     8641 cttcactacc cgctatccgg cggcaaagac tttcaagata gaaaccaatt accgcagtgt
     8701 gccggaaatt ctggaacttt ccaacgcggc tatcgccgcc aacgaagttc aaattgaaaa
     8761 acagctccgc tccgtgcggc ccaccgggga aatgcccccc gccctcgtcc cgctcaacga
     8821 cgaccggatg caggccaaat ttgtcgtcca gcgtatcagg gatctcattg aagaaggcac
     8881 ggaccccagt gaaatagccg tgctttaccg cgcccacttc cacagcatgg aattgcaaat
     8941 ggaactgacc cgcggagaaa tacctttccg catcaccagc ggcatcaggt ttttcgagca
     9001 ggcccatatt aaggacgtgg tggcatttat gcgttttgtg gtcaatccca gggatgaact
     9061 ttccttccgc cgcatggtga tgctgctgcc cggcatcggc cccggcatgg cccaaaaact
     9121 ctggatacgc tggaccgcct gcccggaaag ccggcaagaa acccctccgg aatccttttc
     9181 cgccctgatg ctggatttcc cggttcctgc caaatccaga acggcctgga cgcagctttg
     9241 ctacacactg gatgaactgg cccctgccgg aaagctggcc ggaccggcct ccatgctcct
     9301 ttccatcaat gaggccgtgt acgacgagta catgcaggcc gcattcgaaa attacgacca
     9361 gcgcaggcag gaccttctgc atctggccgg attttccgaa cgctttgaat ccacggagga
     9421 ttttctttcc cagctttccc ttttgggcaa cacggatgat gaaagcgccg cggcggactt
     9481 ctccggtggc tccgtcaccc tgtccactat ccaccaggcc aagggactgg aatggtctgt
     9541 cgtcttcctg atcggcctgt gcgacggcat gtttccgcac cagagggtca ttgatgacgg
     9601 agacctggcc gggctggaag aagaacgccg cctcttttat gtaggcatta cccgcgccaa
     9661 ggaccagctt tacctgacct acccccgctg gaactgccgc gcccagggag gaaccttcat
     9721 gcaaatgccc tcccgctttc tggatgaatt cccagccggc ctggtggaag agtgggaggt
     9781 ggaataatcc agatctcctc tcccgggtca tatccgggaa acgccggttc tcaatcttct
     9841 cccgccggac agggaaaacg tggcgcggag tttccgaagg gttcttttcc cacccgtttg
     9901 aattcctgct tccaaccctt atccggagat atttccgtct ttcccggaga acggaattcc
     9961 ccgacaaccc cggccatatt ccaaaaaccg tggacaccgc ggctcttacc gcatccttcc
    10021 gtttctaccg ctgcggttca tccccgccat atccagcaaa gccaataccc acttccgtat
    10081 tttgcttctc aaaacgcttg ttgggaagca tcccaaaccc tcttttcatt cttgctggtt
    10141 tgcaaaatta aatatatagc tttctgattt tggcgggaaa gcgcttaggg aaatatttca
    10201 tatgcgtttt tgtaagtaag tatttacgga atatactgaa atccccactt cgtcatttca
    10261 tagaatacag gcaacaatgc ctgccccttt tccgtaagag agtattcaac acggggcggt
    10321 atctcgttga attgcattct ggatacaagc ccgtcctttt ctaattctcg aagggaagag
    10381 gcaagcatgg tatttgtaat accattaata ttttttttta actcaccaaa gcggatggga
    10441 tcttgaataa acaattcata aaggatttga aatttccatt tcccctgcaa cataagcaga
    10501 agtggagtca caggacaatt ccccgcttca gtcaaaattc cctctttcac ttcatgcctg
    10561 tattcatcgt aggtcatatn nnnnnnnnna gtcaaccgta ctgagtcaga ataatatgcg
    10621 tacttgaaaa aaattttatc cttagtataa ttatcttact aatagaagtc aacaagtaaa
    10681 gttaaatatt tataatcgtt ttacaatgaa agaaatcaat ataggtcagg cgacggctat
    10741 cacctctccc aatcctctcg tgttggtttg caccaaaaaa gagaacggta tgctgaacat
    10801 ggctcccgtt tcattcttca tgtactcctc gtttaaccct cctatgctgg cctttgcaat
    10861 gggtaaaacc gccaattccg gcgaaaatat ccgcaagacc ggcaaagccg tactcgctgc
    10921 ccctggcgtc agcttgaaag atgcggtcat ggcttacggc tctaaaaacg gcgggcagat
    10981 tgacaaactg aaggaatttc cagtcgctct gcaaaacatt gacggaacag acattcagat
    11041 cccggaggac agccctattg cttttgtggc atcgctagct gaaactgtgg agtctggcga
    11101 tcattacctg tatctctgca atattgacaa aatagtggcg gacaaaacaa aagaagccct
    11161 gttcgcatgg aatggttatg ccaaagttgc cgcggcacag gaaaagtaat ttgatgccgt
    11221 cagcggggct ttcatggttt tctgtgaaag ccctgttctt ttccaaatat caaaatctgg
    11281 cgatggggat gatataataa agtgtataat taaaaaaaag cgacacttat gagccagttt
    11341 tctaattttc aaagtagacc ggatcacatt gaaatccgtg gcgcccgtgt tcataatctg
    11401 aaaaatattg atttagatat cccgttgggc aagctggtag gaattacggg ggtgtcagga
    11461 tcaggaaagt cctctttggc tttgggcgta ctttatgccg aaggttcccg ccgctattta
    11521 gaggcattgt ccgcctatac tcggcggcgt ataatacaga tggcagaggc aaaaattgac
    11581 agtatagaat atgtcccggc tgcccttgct cttcgtcaac gtcccgctgt tcccggtgtc
    11641 cgcagtacat ttggaacggc taccgaactt ttgaactcaa tccggcttat gttttcccgc
    11701 ttgggaaatc accgctgtcc caatggacat ttgctttcgc catcggtcaa cgtcgcggca
    11761 atgcgtcccc ttgtctgctc tgtatgcgga gcggtatttg acggtctcag cgcagaaagt
    11821 ttttcattca atggccaagg agcgtgtcac acctgcggag gaactggaat tgttcaagca
    11881 gtagacgaaa caactttagt gcctgacgag tccctttcca ttgacgaagg agcagttgcg
    11941 ccgtggaatt ctttgatgtg gtcgctgatg actaacgtat gccgggcgat gggcgttcga
    12001 accgatgtac ctttttcgga gttgacagaa caggaacggg agatcgtctt tcatggtctg
    12061 ccagaaaaga aacatatcct ttataaagca aagagcagca atcaagcggg agaaatggat
    12121 tttacctatt ataacgctgt ttataccgtg gaaaatgccc ttgctaaagt taaagacgaa
    12181 aagggaatga agcgggtaga aaaatttttg aagcggaaca cctgcccgaa ttgcggaggc
    12241 tcccggctca ataagcgggc gttatcaagt ctgctatgcg gaaaaaacct tgcggaaatc
    12301 accaaaatga cattaagcga tttaaccgct tggataaaga cgatttctgc ttccatgccg
    12361 gaagaactaa aaacaatggc ggaaagtatc tgcggtcaat ttctgcatgt atcaaaacgt
    12421 ctttctgact taggtctgga ttatattacc cttgaccggg ccggaacaac gctttccacc
    12481 ggagnnnnnn nnnncggaga gcgtcaacgc gtgcagcttg caagggctgt acgaaaccgt
    12541 accacaggtg ttctctatgt gttggatgaa ccgtctattg gcttacaccc agcaaatgta
    12601 gatgggctgt tgggcgttat agatgatctt attgcccatg gtaattctgt gatattggta
    12661 gaccatgatg tgcgagtttt aaaggctgct gattggttaa ttgaaatggg cccaaaagct
    12721 ggagagcatg gcggacagat agttgcacag ggaacgatta aagcagtagc aaaaaaaacg
    12781 agttcactca tcgctggatt tttggcagac aaggaaaaac cggttatccg agaaacggca
    12841 acgccggagg ccatgtttgc acatgggcat atcaaactgt ctactgctcc tttgcataca
    12901 gtccatgccc ttgatttaga catccccaaa ggtcggctga ttgctgtaac aggagtttcc
    12961 ggttccggca aaacaacact gattttggaa agtcttattc ccgccataga agctgccata
    13021 gcgaaagcgc cgatgcctga ccatgtgaaa agcgtggagg tagccggtat tgaacgtata
    13081 aatcttattg atgcaacacc tattggcgtg aatggccgtt ctaccgttgc aacatacagc
    13141 gggatattag atgatttgcg ccggctttat gcagctgtcc cggaagcaaa atcaaaaggg
    13201 tataaagtaa gtgacttttc ctataatact gggtcactcc gttgctttgg ttgtgacgga
    13261 acaggtcaaa ttacaatgga tgtgcaattt ctcccggacg tggatatcat ctgcccggac
    13321 tgcggcggct cccgctatgg gaaagaagcg gaggcaattc gttatcgctc taaaaaagac
    13381 aaggtgaacg cacccggtat ttctctgcct gtactgatgg ggatgaccgt gaagcaggcg
    13441 ttagaacgct tatgtgacgt gaaaaaggtt aaagaaaagc tccaaattct ttctgattta
    13501 ggtttgggtt atttaacttt aggcgaagca actcctgccc tgtccggcgg tgaggcacag
    13561 cggctaaagc tggcggctga aatggaaaag gcacaggacg acgccatatt tgtatttgac
    13621 gagccaacga ttggacttca tccgctggat gtacggatac taattggcgt atttcagaaa
    13681 cttgttgatg ccggtgcaac agttatcgtc attgagcatg atttaaacat gattgccaat
    13741 tccgattatg tggtcgatat ggggccggga ggcggcaaag caggaggaca aattgtagca
    13801 actggtttgc ctaaaaaaat cgctgaaacg gctgaaagcc ttacaggaaa atatctatgt
    13861 gaaataattt catagaaacg taaatctgta ggacgacggt aaaagaaaaa gccgtcgtgc
    13921 aacagcattt tgatactccc aaattaaaaa acggcggtct cgggattctc aaagccgccg
    13981 ttttttatcc tctctctgaa tgtctgacct tcgcggttct gcgcatatca aggctctttt
    14041 cgtaaaatta gtaattgact tctccatatc cgcgaaaata actgttttca agaattttta
    14101 gagataatca aaattgttgc tataaaaaat gaattgacct ggatattctt gaaattcctt
    14161 cccggctcaa aactgaaaaa acatgtcatg aataaattat ttgaagaaga aatataacca
    14221 attcatcatg aataatttta attattattt tcattaattt ctctcatgtc attccggtga
    14281 acgaaccgga atgagaatca tctaaaatct cgggcgccgt gattgtcacg aacgcataac
    14341 catctaatca ttaaacaaat aaaaaagcat gaaattaaga ctcccacata tgctgctggc
    14401 ggctgttatc gcctgccttg gcagtttctc cgttgctacg gcagctgact atgcagtcaa
    14461 cagtgccgat gaattcgtta cggcctggaa ccaggctgcc gcttccaatg aagcttcaac
    14521 catcacgatt accgtacctt ccggctccga taatattacg ctgacccaag agcaacgagc
    14581 ccagttgaac gccatctccg gaacgggcaa cgtcaccatt caaatgacgg acgccagcaa
    14641 caaactggtt aatttcaatt acgatctggt taacaaccaa gtcagtttca acgacgtcac
    14701 cttgagtgaa cctctgggaa gcgacaatga cattgtggtg acgaatgctt caaataccac
    14761 aactattgat ggcaggaata ttgccatcga aggtacgaat gatgctgcca atcccaagac
    14821 ggtgggagcc accgtcacct ccacgaacgg acaggtaggc atcggcgaca atgtttccat
    14881 ggaaaaagcc gttaccgtca ccagccaggc agctcaaacc gtagatccgg caacgccgcc
    14941 gaccggccaa gcctatacca agacgacgta caatacttac aataattcca acgaacaggc
    15001 cgtggtcctg ggcaacaacg tcacgatgca ggataccgta acggcgacag gccaaatcgt
    15061 aagcgatcca gcatccaagg ttgcgctgaa cggagatgtg acctccacag cgggaatcgg
    15121 aaccgtaact acggaacagt ttgacgcctc caacgcccag accggcaaaa ccgtcatcga
    15181 ctctatggat gacagcgtct ccggcggcat cattcttgga gaaaccacag ccggggccgt
    15241 caccctcaaa actgagggcg gcaatatttc cctgggagac aattccgtcc tggatggcac
    15301 tactgtttcc gcagaatccg ccgacctgaa caaaaccgtt tacagcaatg acggcggcag
    15361 ctggaatacc gtcgcaagta cggaaaagct gggcaccatc gaaggcagcg tcacgctggg
    15421 tgaaaatact accgtcaagg gaaattccac cctgacagcg gatgacaata tcgccatcgg
    15481 caacaacagc gtcatcaccg gcaataccgc cgctgacggc gtgatctccg caggtggcca
    15541 gatttccatc ggggacggta cccaggtctt ggataacacc gccaccagcg acggcaaagc
    15601 cgccatcaac ctggcggacg ggcaaaccct gtacatcggc tccggagcaa tcctttccgg
    15661 caatacttcc aacggcgttt ccggttccgt gcaggctggc caaaacaccc agatcaacgt
    15721 ttatacggac gccaccgcct ataccttcat caatgacggc atctccacca cggccgccgc
    15781 ttccacggct gacgccgctc cgctggctgc tgaccagacc gtcatgacga agaccggagc
    15841 gggaactctc gtttacggcg gcacaggaac caccgatacg ttcggaggaa cctaccagca
    15901 gcttgaaggc aacctgatca tcggccatgc caccttcggc gccgttgact ccgccaccgg
    15961 aaaagtcggt ccgctccagt ccattgacgg cgccgtaatg gggacggacg atactgttta
    16021 cgacattcgg acgggacagg tgaccctggc gaagaattcc accatgaagg gagcttccgc
    16081 cactttcgga ggcgattcta ccttgctgct gagcgacggg tccgtactgg acttcggcac
    16141 tcccgccacg ttccaggata attcccgcgt aggcatccag gtatccgacg ccagcggcaa
    16201 tcccgttccg ctcgcccaac tccgcaaggg gacggaaagc gtgacggtca cgctgaacgg
    16261 cacggacatt tccggacgcc tgctgaacaa cgtatttctg agcaccacca tggctccggg
    16321 aacggcggaa ggcaccacca ccatcaccca ggatatgaag ggcattgacg gccccatgtc
    16381 cggctacaac ggcaacgttt acaccgtagc ggccgctctg gaaaacaacc gtctgaatgt
    16441 ggccgccggt tctcccgccg cccagttcta tgaaaacctg ttccgggcca cgagtgcgga
    16501 tgaagcggcc cgcatcatcc agtccgtcag cggtgaacac gtggtgaact tcacctgggc
    16561 agccagccgc accgtgcgga acttcgccga cctggggcgc attcaatccg ccgcctccat
    16621 ggcacgccag acggaagaca ccgttgaagt agtggacgcc aagggttctc cgatcgcccg
    16681 caagacaatc gccagaggca acggcaatat ctgggtgggc ggcatgggaa tctgggatga
    16741 ccaggatgcc cgcgacggtg tttccgggta taagtataat gccggcggtt atgccgtagg
    16801 cattgactac aaagccgctc aaggttccct gatcggcatc gccgccggtc aaagcttcgg
    16861 cagcttcaag gataagacag gcatcggcgc cgactacgac gtcgattcct tcctggccat
    16921 gatttatgga cgcatgcatc ccttcaggga cagcaagttc actctggacg gctacggcgc
    16981 ctacggacgt tcccgtttca aaggtgattc ctacatcatg gggtccgccg ccaacggcag
    17041 agcagatacg gacaccttca gcggcgccct ttacggcacc tggacggaac gtttcgcact
    17101 cggcagggcc tttataacac cttacacggg tattgagttc atgacttccg aactcaaggg
    17161 attctctgaa agcggacctt atgggcgcac cttcggccac gcccgggccc agaactggac
    17221 cattcctgtc ggcattacga tcgcccgcgc ctaccagacg gacggaggca ccaccatcac
    17281 cccggccttg acggtagccg tatcccagga tgtgagccgc atgaatccga aatccaatgt
    17341 cgccggcccc ctcggaacct ggaacgcccg cggcgtcaat gttggccgca ccgcattccg
    17401 tttgaatgcc ggcattgatg tgctcttctc caaccagtgg ggagcacgcg tctgctacca
    17461 gttcgagacc cgcaacaagc tgaccgccca cggtatcaac ggcgccctca gctatacgtt
    17521 ctaagtagga gagcgctcca gcgcatgctt ttacttggtt atggtaggcc ctttcccgcc
    17581 ccacgcggga aagggttttc cgtatgtttc cgcatccgtt cttttaattc ctctgcggat
    17641 tcccaatccg aagtcaacac taaacgataa aaacgagatg tttatacaaa tacatgaaca
    17701 gagtcgtctt ataaccagaa actttcctgc gggaaattta aaaccattat ataattcaca
    17761 attccaatta tttatgcata ataaagagac gtggatttat ttttcggatt gggaatccgt
    17821 tacccgtgct tttttgaagt acctgatcaa catgaaatta tctacattct gcatcttatg
    17881 ttgcctttcc gcaggtcttt cccaggctgc cacctacgtc tggagcggag cggcagggaa
    17941 cggaatttac ggagattcca acaactggac ggtcaacggc gcgccgaacg gctattatcc
    18001 gcaaagcaat agtgataccg ccatcatcgg agaaaatgcc ggaacgatca cctggtccac
    18061 cgggcaatct tatttcggag ccactaacac agtccaaata gactccggaa gcaccctgct
    18121 ttgtacaacg gaaataggag atctcaacgt taattctttc acgctgaaag gcaattccca
    18181 attgatattt gaatccagca acgcccttgg actgggacgt gatttcacgc tgaacttcgg
    18241 cacgtttacg gcggaggaac acggttcctg ggttgccacg aatctcccca gcttctggac
    18301 caatgggaaa accgtcacct tcgtcggcac actggatatg aacaaccttt ccggaagcgg
    18361 aactattgaa ctcgcttcca ttaaatcttc ccaactggga ggcaatctca atttggacct
    18421 gagcggcctg gacattaccg gcaacaacca aattcaagcg gatgtaacac aggtcacgga
    18481 aaacgacatc atcaaggtgt tgattaacta cgaaaccgtt ccggaaccca caacggccac
    18541 gctgagcctc ctgggactgg gcggtctgct gttaagaaga aagagacagt agcctcttcc
    18601 gcatgtctga ttccaccccg gcttattcct caaggaaagc cggggttttc ttttgccgct
    18661 ggcccggttt gcgcacgcct cccttatatc gcaacattta tcctgcggaa ccatatagca
    18721 tccttccaga cggaaaaatc cgttttccag agcaggtcag aactcgcaat tccgcggggt
    18781 acgggcaaaa ggcaggacgt cgcggatgtt ggtcaccccg gtcacgaaca tcaacaggcg
    18841 ttcaaacccg gccccgaatc cggcgtgcgg caccgtgccg tagcggcgca gatccgcata
    18901 ccaccggtac gcttccgcat tcatgcccat gctgcgcatg ttttcctcca gaatatccag
    18961 ccgctcctca cgctggctgc cacccacgat ctcaccaatg ccgggcacca gcacatccat
    19021 ggcggtaacg gtctttccgt cttcattcag gcgcatatag aagggtttaa tctccttcgg
    19081 gtagttgtac acgatgacgg ggcatttaaa atgctcctcc gtcaggaagc gttcatgttc
    19141 gctctgcaga ttgatgcccc aggaaacggg gtaatcaaat gttttgccgc tcttgagcag
    19201 gatatccacg gcttccgtat aggaacagcg cacaaacggc gtttccttca catgctccag
    19261 gcgttcccgc agccccttgt ctacaaaacg ggtcaggaat tccacttctt ccgcgctgtt
    19321 tgccaaggca tcgccgacga gaaaacggac aaactcttcc gccatgtcca tgtctccata
    19381 caggtcgcag aatgccattt caggctccac catccagaat tccgccgcat ggcgcgtagt
    19441 attggagttc tccgcccgga acgtggggcc aaaggtataa atgttgctca gggcgcacgc
    19501 gaacgcttcc ccttccagct ggccgcttac cgtgaggtag gccgccttgc cgaagaaatc
    19561 ccgggaggcg tcacgggaag cagcgtctcc cacatccaaa gtcgtcacgc ggaacatttc
    19621 tccggcccct tcgcagtcac tggcggtaat aatgggagtg ctaacccagg aaaaatccct
    19681 ttcctggaaa aagcggtgga tggcgaaggc cagcctgctg cgcatgcgga acacggctcc
    19741 aaacaggttg gtgcgggggc gcaaatgggc gatggaacgc agaaactccg gcgtatgccc
    19801 ctttttctgc aagggatagg aatccggaac agcccccacc agctccaggg aagcggcgcg
    19861 caattcccat cgctgccttc cctgcccggc aaccagagcg ccgcggatgc tcacggcaga
    19921 ccccgtagcc atttctccaa tccgttcata gccggggata ccctcatccg ccacgacctg
    19981 cagggaggcg acggaggaac cgtcattcac ctccagaaag gaaaaagcct tggaatcacg
    20041 gcgcgtacgc acccatccct gaatcagtac gtccggcatt tcctcctcac tgtccaggac
    20101 atgtttcacc aatgttctgt tcatcatggc gcaagtataa tacagcgcct tgcccatgcc
    20161 agcagaattg cttcaccacg gagagaatat tacggatttt ccggcagaga aggaggggat
    20221 atgccgcacc tgttatttgg aaggctgcat gacctgccgc cccgtcgtaa tggcgatggc
    20281 atccgccagc ggcatttcct tctccgtatg ggcggccccc caggaatctg aaaagataag
    20341 cgttttcttt tcctcgtcaa aaccgataat cagcctcaaa tggcctccgc gcgtctggga
    20401 caaacgcaac ggttccggaa caatgccaag ctgcacggac caaaacacgg ggatgcccgc
    20461 catgatatag ggcctgatgg cattgagcca cctgtccacc tgggacggtg atccggcacg
    20521 cgccagcttc aggacttctc cgtccgcatt gtcccagaaa ccggaccagt catgttcatt
    20581 ttccaccttc tcttttttca gcttggaggc ggcgcggtta taggctttca gcagattcct
    20641 gaaatcacgg gaattcgcca gggaatccag cactttgatc cgtatctgga agcgcgtccc
    20701 gatttttttc aggttttccg ccattgccgc cgtattagtg cctccgtccg cagaggtatt
    20761 ggccagggaa gccagttcat gctgatccac atagtccatt ccataatagg caaaaatgcg
    20821 ggcggcggta gccaccacgc aataaccttt ctggccctgg tccaccatgg gaatatcctg
    20881 aatcaccacc cgctttcctt ctttcctgac gtgctgcttg atatccgcct ttctggcccg
    20941 ggaagaagca tctcccctcg cgatggaatc ttccgtggga ccggccttca tccggataaa
    21001 ttccgcttca aactccctgc cctcccgcga tgtattgatc tccagggtaa tcgccccgtt
    21061 gtcccaaatc caggaccagc cgttgacttt gactaccgcc tccttacggg aggcccggta
    21121 ctccttgggc tgaatcccgg taagagcggt cagggattcc ttgatgagct ccaggcgctt
    21181 gtcaaattca tccttgccaa tggccccgtt atcccccttg ttgtacatca tgacagtcat
    21241 tgactggggg gctccgtcct tccagttcac cagcatttcc cccagggaca tgtcccctat
    21301 cttgatggag ggcttcgcaa tccggagctg ggtcttgcct ttatccaccc agccgccggg
    21361 ctctccgtta aaatactttc cgaacaaatc cggctgaggc agcttccaga acgcaccaga
    21421 ctttaaatcc ggcgccatgt tgcccgcgcc ccaagcggag ggaagaaggc agcagactga
    21481 aaggaaaggc agaatggcag agataaatcg cataacgaaa aaaggggcgg ggaatagccc
    21541 cgctgctcgg aatctacgct ggtaatgtat tctcccatga cggttctgcc aagaaaaaat
    21601 gaatcttcct ccagcgaacc attccttctt tatcctctcc catccgtcct tttctaataa
    21661 tgctgcccgg taaaaaaacc ggcctgccgc agtaaattcc gcagcaggcc ggtttgaatg
    21721 aataaaccag tccgatgatt tacatgaacg gacgcaaacg ctgcatgata gcgtccttgg
    21781 aagaagctcc caccatcgta tccatgattt ccccgtcctt gataatcaga agagtgggga
    21841 tagtgcgcac gccataggtg gcggccagtc cgttgttggc gtccacatcc actttgccca
    21901 ttttgatctt accggccaat tccgtagaaa gctggtctat aatcggggcg atcatgcggc
    21961 agggcccgca ccatgtagcc cagaaatcga caagcacagg aatgtcggac ttcagaactt
    22021 catcctcaaa atttgcttca gaaaatacgt ttgccatagt accgcttaga gtatttcaac
    22081 aatcattacg ttcacttatt cttccgcatc ggcgccatca tcggaagact cttcggcaga
    22141 aacgtcatcg gccgtttctt catcggcggc gggagcagcg ggggcaggct tggagtctcc
    22201 gcccagccag ccaaccatgc tgcgggcttc caaggtgtcc gtagcgtatt gataaccgat
    22261 gaaaacaagc gcaaaggcaa gggccacaaa ggagagaaca ttcaacagcg tactgggagc
    22321 cgcatccgta tcttcttgaa acttcctggc cgtaaaggag gcggaagaaa ccggagaggc
    22381 cgcgggtgcg gtattcagcc cgatggtggc ctgcggaagc tgtgctgtcg ccatgcctgc
    22441 tccgggagga gtcagaggaa cagtaggagc ggcagcggca ggcgccgcgc cagcaggagg
    22501 aaccacgggg gccacaggcg tagtggcgga gccgagggca accgtcttta atcctccgcc
    22561 aagcttgatg cctccgccaa gaccggcggg gcgaagggga atagtgggcg cagcggcggc
    22621 aggcggagca gcaggtgccg caggcgcggc ggctaccgga ggaacggccg gagcggcagg
    22681 tgccttgggc acggaaggag cagccggcgg agtcggaatg ctgggcggag gcggagtacc
    22741 gcccggaatg gaggggggag gtggattact catatcggaa aaataactgt gctgaacgat
    22801 agctctcgct tatttcagcc cgtaaatcaa gaaaaagaat gcgtagaacc tgaattccgt
    22861 gcaatttttt ttaatttgaa gcgtattcca tctcaaacac gctgtccgga cttgccctga
    22921 aaagcgttct ttcctacgct gtgtatccgg cattatctca tatgaaaccc attaccaaac
    22981 tagcttcctc caccctgccc gacggctccg ttctcgacct atgggaacgg gacggtttcc
    23041 actttctttt gcgcgacggc gtacagacag cgtccagctt ttcccacgga agcaatgaag
    23101 cggcggcagc catggcggcg gctccggtta aaagagccaa ccagccctcc ttccttctcg
    23161 acggcctcgg cctgggattc accctgttcg ccctgatgga tcaaattcgc agggaaaagg
    23221 cccgcttcat cgtggcggaa ccctgcaaac cgcttatcga ctggcacaag gaactgatga
    23281 accaggaacg acccggattc ctgcacgatc cccgcgtcag cgtggaattt accccggcgc
    23341 ttcaaatcgc ccgcaagaat ccgaaaagct tccacgccat tctgtcccat tccacgcacg
    23401 aacgcatgaa cctgagcgta gccgaagctt ccgactattt tgccgcactc aagcagggcg
    23461 gtctgctggt cattacggta gcccgcccgg acacccgact ttcccggaca ctgcaaaaag
    23521 cggggttcga agtaagcacg gaagccgttc cggcctcaca taaaggaaag aaaaccgctt
    23581 ttcacaccgt tattcttgga aagaagggac gtttcgtccc cttctccgct caccagtcct
    23641 gatcccatcc tattattatg aactgcacat cccttaccat ggccggactt ctggccgccc
    23701 tgtccctggg aattccctcc ctggcggatt ccattgcccc gggcattctt cttcccaaaa
    23761 aaaataccaa atccatttcc ttctccccca aggggacctc ggacattttc ctgctcggca
    23821 tcatgccgca gggaagttcc gtcaagaaag gggaatccgt agctcaggcg gacttccgca
    23881 tactggacaa tagtatcgaa gattatgaac gcgccatcaa gagcaagaac ctggaggtgc
    23941 tgaaactccg gtacgccctg gaccagcaga aggaacgttc cgccctggcc ctgcaaaaat
    24001 atcagacggc cctgacacgg acggaagagg atcaaaagga ttttctggaa aagcggaagg
    24061 cccgcatgct ggcggaagaa gaggaacgcg tcaacaaagc cctgcgccac atgtcctaca
    24121 aacaggaaga gctgaaccag cttaccaaga tgtacaagga tgaccaggtg gcggaagaaa
    24181 cggaagagat cattttgaaa cgcctgaaaa atgaacttgg ggaaagcgaa tttgccgttc
    24241 agggagccaa actcgttgcg gaactggcca agctgcgcaa tattcatcgc ctgggggaag
    24301 actatgctac ggcagtcaag gaaaagcaga tggatctgga acaggcaagg aagcaggcgg
    24361 attttgatct ggaacagaaa aagatagccc tgacggaggc ggaagtctcc ctgagacgcc
    24421 ttcagaataa gtacaatgaa ctgaaagcag accgggaaat ggcctctttc aaggccccgg
    24481 aaaacggcat cctgctgtac ggcggatatg tggcggacaa gtgggtggcc attccggtgg
    24541 cggaaaagct gaaacccggc ggcaaactgg gagcttttga caaaatcgcc accatcgttc
    24601 caccagactc ggaactgatt gtccaggcta ctcttccgga ctccaccgct acgcccaagg
    24661 tgggggaaac cgtcgtcatc aaaataacga acatgcagat tcccggcgtt attacggaag
    24721 ccagccccat tccgggagca gacggcaagc gccgcattat catcacgccc aaggttcccg
    24781 ccagccagat tttcgccccc ggccttcccg tccaggtgac catcaaggac caacaggcct
    24841 gatttccctt aacggatccc catgaataaa gccgtccttt tccttgccgc ccttggagcc
    24901 agtacggcgc tctgcgctcc tgttccggcg gaaaaagcgg aaaaaaggga taccattccc
    24961 attgccaggg ttccggatgt tccgcctccc tcccgggaaa cgctggacgc ttctatccgg
    25021 aaggcggctg actttctgtt gaaagaacag aacagggacg gttcctgggg aaaccatacg
    25081 cgcaccaagg gtctgaatgt tctctgccct tatccggaag gccccagatc attcaggaca
    25141 gcctccacct ctttgtgcgt gatcggcctc ctgaccagcc ccctgaaaga agaccccgcc
    25201 gtcaaggcat ccctggacaa ggcgctgcag tacctgctaa ccacgctgcc caccctgaaa
    25261 cggggagaca cccgtaccgt gcttggcgta tggggccatg cctacggcct ctccgccctg
    25321 agccgggcgg cgcggaacct tcctgcggat gcccctttac gcgaagaact caaaaaggtg
    25381 gcctccctcc aagtcaaagc gctggaccag atggcggacg tccaaggcgg ctggggctat
    25441 tacacatttg aaactttttc caagcgtccc attggagaac ccacttcctt cctgacggct
    25501 acagtccttc ttgcctacaa ggacgcggaa caggcctttg gcctcaaagc ggaaccccgc
    25561 atcgtcaaac gcgccgtcgc ctgcctggaa aaactgagga cgcccgccgg cagctacgtc
    25621 tattcccttt cccattcctt ttatcccggc aggcccatca accgccacac cggcagcctg
    25681 gcccgcactc ccgccgggga tctggctctg atgcggtatg acccgtcctt tatttccagg
    25741 agtcagctgg aagacggcct ggaacgcatc tggagccgcg gcggctggct ttcgctggct
    25801 gtaaagaagc ccattcccca tgaaagcttt gcccagaacg cgggctattt tttctattac
    25861 gggtattatt acgccgcccg ctgcctggag gaagtgacgc cggaacacct ggcgcgccat
    25921 gcggcccatc tggcaagcga cctcctgccc atgcaggaag aagacgggtc ctggtgggat
    25981 taccccctgt acaattacca caaattctac ggcaccggat atgccttata tgccgtctcc
    26041 cgcgtcagag aatccctgta tccctctgat gcggcaacaa cttcatgacc catgttccgc
    26101 ccgctgacga ccatttttct cgccaccttg tgcctgcatc ttcccgcagc gcagggggaa
    26161 tccgtgccag aggaaaaaac ggatgttatt ccaatcgcca aaatctcgga tatttccccc
    26221 gcaaaacccg gacaatttga cagggcattc cgccgcggcg tggatttcct gctgaaaacg
    26281 caaaataagg acggctcctg gggcgaccac cgggtaatcg gcacctggaa catcctctgc
    26341 ccctacccgg acgggcccct caccttcaaa acagccagta ccgccctgtg cattgcaggg
    26401 ctcaacgcca gccccctgca tcatgaaccc gtcgtgcagg aagccatgac ccgtgctgaa
    26461 gactatctaa tccgtacgat gccccatctg aaacgcggcg acgctctgtg cgtttacaac
    26521 acctgggcgc atacttacgt gctggatgcc atgagcatga gggctgcaag actggccccg
    26581 gacagccttc gctacaggga actgaaggaa tgcgcccgct cccaggtaaa aaaacttaat
    26641 gaactggctt ccgccatggg gggctggggc tacctcactt att
//
LOCUS       DAWEMX010000018        26624 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_748_length_26624_cov_11.944973, whole genome shotgun sequence.
ACCESSION   DAWEMX010000018 DAWEMX010000000
VERSION     DAWEMX010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 26624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26624
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_26624_cov_11.944973"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4369..4378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15178..15187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5512 a         6900 c         7773 g         6419 t
ORIGIN      
        1 gcgtgatgaa acatttgccc ggcgtgaagc attgtgacgt caatattgag cataaggctc
       61 cggaagccag gaagggaatc aatgacgacc cctctacctg gaaatcctcc gttcccggag
      121 ccaaacatgt cattgccgtg gcttccggca agggcggcgt gggcaaatcc accgtttccg
      181 cgaacctggc ggtggccctg agcaagctgg ggtattccgt cggtctggtg gacctggata
      241 tttacggccc ttccatgtcc ctgatgttcg gcaccaagga acgccccgga gccaatgaga
      301 atgacgaatt tatcccggta acggcccatg gcgtgaaact tctttccatg gggttgctga
      361 ttaatgaatc cgatcctgta gcggtgcgcg gtcccctggc gacacgctac gtccagcagt
      421 tcctgcgcaa cgtggcatgg ggggatgtgg attttctgat tctggacctg cctcccggca
      481 ccggagacat tcagctgact attgtccaga cggccgaact ggacggcgtg gtggtggtga
      541 ccaccccgca ggaagtggcg ctgattgatg cccgcaaggc gatcggtctc tttgaacggg
      601 tggaaacccc cattctgggc ataattgaaa acatgagtta tttccggtgc ccctctgacg
      661 gcaagattta ccatatcttt ggcgaaggag gcggcgaacg ggaagcggcc aggctgggag
      721 tgcccctgct cggcaagatt ccgctggata tttccacccg ctccggcggg gacgaaggcc
      781 gccccgtggc cctggaagag cccgggcaga atccggtttc cgccgcgttc cgccaggtgg
      841 ctgaacaatg cgcccgtgtg gtgcttgacc gctgaatgcc cggacggggc gggaggcccg
      901 gtacggttgg caacctttaa ctttaaagcc ggattggagc ctgccgctct ggtccggctt
      961 gtttgccggc atgttccggc tttttgagtg aggatgcatg gaagggaata aaaaaaatga
     1021 ataagacgtg gaatttgaag gaaatgaaaa agttgatacc gctggccctt ccggtgctgg
     1081 tggtcaacct ctccattgtg ggcatggggg cggtggacgc tattgtggcc gggcgcgccg
     1141 gcgtgacgga catggccgcc gtggccctgg ggtcttccgt gtacctgccc gtggctctct
     1201 tcgcctgcgg cgtgttgatg atcatcggcc ccgtaattgc caacatgcgg gggaaaagcc
     1261 atgaaagccg cgtgggctac atgaccaacc acggcctgtg gctggcgttc atgctcagtc
     1321 tggtttccat gccggtcatt tatgtgttga gaaatgtgtt cggctggatt tccgatgacg
     1381 ccgccatgtg ccagatggcc tccgcctaca tgttcgccat tatgtggggc cttcccgcca
     1441 acttggggtt cgtggccctg aagagcctga acgaaggctc caatatgaca cgtcccgcca
     1501 tgtacgtggg attgtgcggc ctgttgctca acattcccct gaactacatg ttcgtctttg
     1561 gcatgtacgg ttttccccgc atgggcggag cggggtgcgg cgcggccacg gcagtcattt
     1621 tctacattga attcctgctg atgttcctgc tggtttactt caatcccaag cacaggccgt
     1681 accgcaggca catcatttcc tggcggcggc ctacgccttc cgtcatcacg cacctggtgc
     1741 ggctgggcgt gcctataggc gtttcccagc tgtgcgaggt gatgctcttc tgcgcggcgg
     1801 ctctggtgct ggctccgctg ggagagacgc aggtggctag ccaccagatt gccgggaacg
     1861 tgggcggcct ggtgttcatg ctcccgcttt ccgtagggct ggcggcttcc atccgcgtgg
     1921 cgtaccatca cggcaggaat gacctggcag gcaccagatc cgccattctg tcttcctatg
     1981 tgctggtgct caccatctgt ctgtgcacct ttggaggcat caccctgttc cgcgagcaga
     2041 tcgtgcacct gtacaatgac tcggagctga ttgtcagcac ggcttccgtc ctgctggttc
     2101 tggcggcggc ctaccagctt ccggactgtt tgcaggtgct ttccgtcggg gttctgagag
     2161 gattccggga tacggcgtcc attaccttca ttacgttctt ctcttattgg atagtaggat
     2221 ttccggcgtg ctacatcctc gcccgtacgg actggattgt cccggccatg ggagcgcggg
     2281 gcatctggac gggattcatc atcggcctgg cagtagcggc ggttctgctg ctctggcgcg
     2341 ttgtccgtac cgttaaacgg gagtttaaga tgatgcggcg ccatgaaccg taatgggctc
     2401 acctcccgtt tttatggaga ggttaatccc cctccttttt cgggattgcc ttctcagcag
     2461 ggatgagttt tccttgttcc agaattcgcg ggtaggggaa gagctttttt tctttgccaa
     2521 attttacggt cattcggccg acagcttcgt ccacctttat tacaatgccc gggccgtatt
     2581 cccggtgggt aaggagcatc cctttatgaa tgcgcgcatt cggagcgggt gggggggctc
     2641 cgccttcttc ttccagacgg caggcaacac tgttgtctat cgttggggtg ggaagggctt
     2701 tttcctctat agagccagcc atggaatcct gctgggcacc agccatcatt tcgtagtctt
     2761 tccggttcag gacggtagcg tacagcccca cgttttcctc cgcgtggcga ttgatacccg
     2821 tataaacaag actggcatct tcataccgtt tgaacttgta cacggcatca ttcatcaggg
     2881 cctcattaaa gacgttcagg ctgacgagca gttcaaaatc tttttccgta agcccggtta
     2941 ctttccggaa cagatgcggc tccagttggg taatcacatc cttcagacag tattcccgga
     3001 agtctgacag gtacatgaag atggggattc tggcggcaaa tttgatcagt ttctgctgta
     3061 tctgcctgcg cagggatttg atttcctttt cttcctgtga gaggtctttt ttttccttag
     3121 gggtcagatc gtctttttct ttgcgcattc ctttgacggc atctgatttg ttgataatgg
     3181 tctcaagatc actgttcagg ttgcggaagc cttcaatgct catcagggca tccatggctt
     3241 cccggctggc aagcaggcgt tgcagggtga cgttatccac attgaccagc agggcgcttt
     3301 cccagcgtct ggccagcagg gtggcgctgg ttcccgccat ggccatatcc agtatttcag
     3361 aggcttccac ctgtttcatg gcgctgccgt cgtaggccag cacgggaagg aagctgatga
     3421 actccttcac cttggtttcc ggattgcccg ggttggcgtt caggcggcag ctgtagtcag
     3481 ctatttgttt aagggcgcgg tccagcgcaa aatcaaagac gtagcaattg ggtttgacga
     3541 tttctttttt ccccggctga tcactgtcca ccgtccacgg gctttgcacc cggaaggctg
     3601 cctggaagta ggtttccggg ctggagaggt tgcgcagcat gaagattccg gaccagggct
     3661 tgacggtgat gcccgtggtc agtttgccgc aagagagcgt aatggttttg ctttccagcg
     3721 gattgccgcc catggccttc aacacgggcg gcagggcgtt cgcccccgtg ccggcttccg
     3781 gtccggcgca gacgatgact tgatagtcat ggtaaaagat attctggcgt tgttgcagca
     3841 ggttgttcat ggcgtggcag gatgccacat ccggcaggaa ccacagggta tggttgagca
     3901 agctcatcag ccggatatct gaataaggca tggggggctt ttttccgccc agcttcagct
     3961 catccgtcgc cgtggcatga tgggagccgc ggatcagatc cagccatttc tggacgtact
     4021 cttcatgggt aaagcgggct tcactccctt tccctttcgc ctggaagaag atgttcaagt
     4081 caaattcatt gaactctccc cccagagcga tttgccggat gtcttccggc atgcggtagg
     4141 tgagcagcac catgcgcggc agggcggcat acgggttatt ttctccctgc caggctgcct
     4201 tggcgcgttg ttcatcggaa tatgtccagc tgtagatttg ttcttcaata aattctccgg
     4261 agttcaaagc gcggaacggc gttccggaga ggtacaggta gtggtccgcc gtgatgggca
     4321 gatcctgttc atcgcagaca ttgccgctgt tggtttccgg gagcgcggnn nnnnnnnntc
     4381 ttcctcatcc tccatcagga acagggcttt ggcgttttcc ttccacgctc caaagtggta
     4441 ttcgtcaaag atgaccagat cccagttgat ttcccgcacc cattcatgct ggggcttgat
     4501 gccgcccgtt tctttattta cccccaaaaa gtcctgaaaa gaaccgaagc acacaatagg
     4561 ccgcccggcg tccgcctgcc ggtattgcgc gtcaatgggc agggcctccg gaccttgttc
     4621 ccggcagatg aattgccagc cttcaaagtc cagatgtgtt tccaggtctt ccttccacgc
     4681 ggtttgtacc gccggcttga aggtcaggat gagtacccgg cgcgcattca gcctccgggc
     4741 cagctggtaa gcggcaaagg ttttgccaaa gcgcattttg gcattccaca gaaaacgcgg
     4801 cgtccgtcca tccgcgcgtt ctgccttaaa gtagttcagc gtccgcttca cggcttcctt
     4861 ctgttccgga cgcatggcaa aatccctggt acggttttcc atgttggccg ttcgggtgcg
     4921 taccgccagc catgccgccc tgatttcttt ctccgtacag cggaaccatt ctccgcccac
     4981 tcgctccatg ccttttttct ccagcatccg gtgtatggca tggtccgtaa agctgctccc
     5041 gtcttcatgc agcgccggtt ccgcgaagat aacccggtag ggtttcttcc cttccggcct
     5101 caaggtaggg tactgttgcg ctacgcgttt ttccacatcc accgctgtgt accccacttt
     5161 cagacaaccg gggtagctgg cgtcctcata ggcataaatc attggttgtg cgctcgtttt
     5221 ttgttcaaag aaacgagcac tgttttccat agttctttgt gtaaaaaact caattgccac
     5281 ttgagttacc ctccaatctc cataggttta atcaatttct caatatagtc tatttcttcg
     5341 gtttttaaag aatacttcga atatagcatt tcatctgtcc aaggtttttc aaaaccgaca
     5401 taagggacaa aacagaattt attttttgtt atatgaatgg acgaaagagc ctgtaacagc
     5461 aggaaacgag caaatttact ggatatgaaa gaaacaaggt tttctgcttc ctgctttttg
     5521 tcaaacgcat ccaagatgag gtatgtttca gtacaggctt cttttggatt gagaatgcgt
     5581 aatgaagata atatttgata atttccttcg gatgtaggct tattaccacc agacatagca
     5641 taggtgataa tgactttata tttatcaagt atgccttcct tatctgtcac agtacttttg
     5701 tgcagataga tgaacccttt ggaggtgaaa attttgatag tattaacccc gaattccttg
     5761 ggaccgcaaa cagtagttgg cagattgaaa taattacgag agtacacgac tttgttcata
     5821 agtttacctt tacttgctag aacctttcta ataatcgtta atgcaaggtt gctgcgaaca
     5881 aagtaatcaa actcatccaa ggcgcgaata ttctctgaag tgtattctcc ctcctttatg
     5941 ttaacaacgg cacagtctcc cgaataagtg ttatcccaaa gaaaatagca gattcctcct
     6001 gcaatattta caccagggaa gcagtcagca ctgttggcaa catctacaag ttttcttata
     6061 tgtttttgac cgagcatagt tgctcggaag tcatctaagc cctttcctcc agcataccat
     6121 cttgcaggga tgaccataga caaatatttc ggtttaagtt tcatcgcctg ttcaacaaat
     6181 aaatgatata atggtttggc acttgctcca ttccctccat cgtcaagttg gtagggagga
     6241 ttgccgatga tgacatcaaa gttcatagga aacaggttat tcggattgtt gatgtggatg
     6301 aattcgtaag catggctctc cagaccctct tgtcgtgcct gttctccata ggcggcttgg
     6361 gaggcgccgc aaaagacgca tttgccgccc ctccaggagt gtgtgatgtg tttgtagcgg
     6421 atgttgccgt cggcattgtc aaaacgaacg acggaataag gcccattggg gtatttggag
     6481 cagtacactc cgcggcgggc gaggagggac gtcagctccg taatggcgat gccgaagagc
     6541 tgatttctga aaatgtgttc caggcgatcc tcccggtttg ggatgtgagc ttccagcccc
     6601 ttgttgagcc gtttggcaat ttccctcaga aagacgccgc tcttgcagca ggggtccaga
     6661 aaggtggcgg aggggtcgga aaaaagctcc tgcggcagca gatccagcat ggcattggca
     6721 atttccggag gcgtgaacac ctcatcactg gaaagattgg ccaggcagct caacacgtca
     6781 gggttatgga tgccgttgct catgattctg cttccggcag tttacggtaa tccatgggag
     6841 ggaactctcg taccgggtat ttgggtacgt attccagagg aagttcccct tttgcctgtt
     6901 ccagggaagt ccgggcgctt tgatcacgca ggagttcatc ttgccggaag tcccggcgtt
     6961 tgatgagggc gccgcccacg gcggaccatt cagaaaagat gatggggtct tgcgtatcct
     7021 gtgcggcgtc atccacctga ttcatgctca gggcgttgcc gttaagaata ttgcgttcca
     7081 gaacaaaacg gacggttttc tggcattccg cggagggcgg acattttaaa cgttgttcat
     7141 attccgccag ccacaggatc agcaggtgtt cccggcaggc gcggacgtta tccgccagca
     7201 gttccacacc gtagatagac atgagagcca gcacggcgcg gaattcataa tccgccggag
     7261 aactgccgta ttgccggcgg acggatgcca gcttcctcaa caggataggg gccagaaagg
     7321 ctccttctcc gcaggccggt tccagaaagc ggctgtcaat gcgttccgct tcatgcgcca
     7381 ccaggttgag catgtcattc accagccagt caggggtgaa tacttctccg tgatccgtta
     7441 cccgtttacg ggatttaatc tgagaaggag tttgaggatt gggcgacatg acgcctctct
     7501 tttcgcacgg ataggctatc cgcaagattt gtatagattg attttctttg gggaaagagc
     7561 aacggcgcaa gtatttgtgt acttagtcaa tgaatgagta ttgccgttac gtttttgttt
     7621 ccttatcttt ttttattgct ctgaccgttt tatacttgat gcggatagcc tatccgtttt
     7681 cctatctttt cttcatgact caacaggaat tgacggaaga agaagccctg ctcatttgct
     7741 ggatgcgtaa aaaacgcgtg caggctgcgg aaataatggt catggcgcgg ttgctccggg
     7801 gtgtttcaac cgggatatcc gggcggctgc aatgcgtgga gctggggatt aaggaatgga
     7861 aagcgatgaa aaagacggtc agtttttccg tttgcgtggc ggagtccctg aaagcccggg
     7921 cgcatcggcg gcgacgcacg ctggaagaaa tccggtacgt tacggcgcgg atggtgaagg
     7981 cgtggccgga cctgagacat aagatgatgc ggagcctgag gcgggaagat tgccagcgca
     8041 tcctgaatat attcccgacg gccagcggac ggcgcaaggc gcgcatcatc ctacacggaa
     8101 tatgtgcttt tgcctgccgc aggggatggg cggggaagaa cccggcagcg gagacgccgt
     8161 gtccgcatgt gaaggaaaga agggtgcctg tgctgacgcc ggaggagtgc cggaggctga
     8221 tgacggcagc acaggaggtg tgcggtggcg agtgtctgcc ggcagctgcg ctgatgctgt
     8281 atgccggagt gcgtccgcag gaggtcagaa ggctccaatt ccggcatatc aggctggaag
     8341 aaaaagcggc gcttattccg gcacggcatt ccaagacggg aggggcgcgg cgggtgaccc
     8401 ttcggccggc agcgttgcgg tatttgcggg ctttccggca tcattctggg gaagaaccat
     8461 tgtgcccggc aggatgggaa cgcaagtggc ggctggtgcg tgcccgcgcc ggctggaata
     8521 actgccggcc atgggtgcag gatgtattaa ggcacacgtt tgccagttac cacgcgctgg
     8581 aattccggga ttatgccgga ttgcaatggg agatggggca ccaaaacgcc catttattga
     8641 aaaccagata tctgaatatg gatgaaatca aaaggcagga tgcccgtgcc ttctggctgg
     8701 cggcgtaagc cctccttctc gggaaaacag agaggttgaa taagaagcag ggacgttcat
     8761 gcaaagcggg cggtcccctc catgaaggga accgcccgca aacggtttga gcagatgctt
     8821 cggagatttt agccgatgcg ttccactttc acggagtggg cctggccgtt gatgatgatg
     8881 gtcaggtcac gggaaccgga agggccgctt acccgggcgg tgattccgtt gtcaatgacg
     8941 gtcatggacg gcgcggcaga cgcaggcgtt gcggcaggag cggtgtttac ctgtacgggg
     9001 gcttcttcct taggttccag ctttttgtag aagtcttcca cctgctgcgg gaacatggcg
     9061 tagatgacgc agtgttcgtc gtccgtgggg agcccctttt ccgcaagctt cttgcgcagg
     9121 tcttccatgc cgggcttgat caggtccgcc ggacggcagg tgatcgggtt cttgcccatc
     9181 ttttccgcgc agagtttctg cacttccggg tctacgggag ccgaggtgcg gccgtagtag
     9241 cccagggcaa tgtccgccgc ctggggggtg atgttcttcc aacggccgaa cttgacgttc
     9301 agcatcgcct gcacgcctac gatttgggag gtgggggtca ccagcggaat ccagcccaag
     9361 gccttgcgga ctaccgggat ttccgcgaat acttcctcaa atttgtcttc catgccctgt
     9421 tccttgagct gggtgcggaa gttggaaagc atgccgccgg ggacctggta gcggagcgtg
     9481 tcggagtcca ccacttcgtt gcggtgggag gcgaatttgc ccagaccttc atagacgggt
     9541 ttgagcattt cggaaacttc aatgagcttg tccgcgtcgt aatccggctt gcggggattg
     9601 ccgttcagca gggcaagcat gcgcaggcag tccggctggc ccgtgccgtt ggcgaagggg
     9661 gagatggaga catcgcacgc gtccactccg gcatcaatgg cgctgaggta ggtggaggcg
     9721 ccgaggccgg cagtgtcatg cgtgtggatc cagacgggga tgttcaggtc tttcttcagg
     9781 gcgctgacca gttcctgggt gacatacgga ggaatgaggc cggccatatc cttgatgacg
     9841 atggcgtccg ctcccatatc cgcgagttcc cgccccatgt ccacaaaagt ctgcgtggtg
     9901 tgcacgggag aagtcgtgta gcagatggtg ccgtgggcct gagctccggc tttcttggcg
     9961 gcgcggatgg aggtttccat gttgcgcggg tcgttcacgc agtcgaaaat gcggaagatg
    10021 ttcatgccgt gcttggcgct catggcgaca aaggcttcca ccacgtcgtc cgcatagttg
    10081 gtgtagccca cgatattctg gccgcgcagc agcatcatgt gcggcgtctt gggggccgct
    10141 tttttcaggg cgtccagccg gtcgaaaggc cattctttca agaagcgcag cccggcgtcg
    10201 atggtggcgc cgccccaggt ttccagggcg ctgaagccca tggagtccag aatgggggcg
    10261 atgggcagca tgtctgccgt cttcatgcgg gttgccgcca gggattgatg cccgtcgcgc
    10321 agaacggtgc aattgaatgt aacaggattc attatttagt taactgttgt taaaaattga
    10381 agaggtggct tctggagggt gattttctca cagcctccct tgcaggtcaa gcatacaaca
    10441 gcggattcgc cggaagaact actggacggg ggccgtcagt cgcgccaggt tgcagagcat
    10501 tttgaagccc agggatttgt tgttgtaatc cgccagggat tccttcttgg aggaggaaaa
    10561 atcctgctcc agcatggctt ttacctccgc gcaggcggag ggggaatgga taacggcggt
    10621 gatttcaaag ttcagggcca gggaacggtt gtccagattg gcggttccca cggtggctat
    10681 gtcatcgtcc atcagaacta ctttctgatg caggaacccc ttttgatagg cccagagctg
    10741 gatgccgtat gtgtccagat cccgaaggaa agtaaaggag gacagtttca ccagaatatg
    10801 gtccgcccgc tccggacgca ggatgcgcac atccacgttc cgcagggccg ccgcctgcag
    10861 ggcggccatg actccctcgt ccgggacgaa gtagggggtg gatatccaga gccgccgggt
    10921 ggcgctgttc gccagggcga tgagcgcggt tttccaggcg ggaatgacgt ctgcggggcc
    10981 ggagggcagg atgagcaggg tttcatcctc cggctggggc gtgatttccc agcggaggcg
    11041 gggaagggag gaggggccgc atttcagcat ggcccagttc cagtcttcca gaaagctgat
    11101 ttgcgtttgc tggacggagg ggccttcaag ctggacaaac gtgtcgcgcc agtagcccag
    11161 agcgcccttg cccaggtatt ccctgcctat attcatgcct ccgatgaagg cggtggaacc
    11221 gtccaccacc accagtttcc ggtggttgcg gaagttcagc cgcaggatgt tgctcaggaa
    11281 atggcgcttc ccgttgaaag gttcaatgtt cactcctgct ttccgcaggg cggaaatgta
    11341 accggggggg agcttgtggg agccgatttc atcatacagc atgtaaatgc ggatgccggc
    11401 cctggcccgt tcaatcagca gatccttcag gttctgcccc acggagtcgt ttttgatgat
    11461 gaagaattca atcaggatgt aatgctctgc gttcttgatg gcgtcgtaga tgcgcgggaa
    11521 agcgttggcc gcgtcaataa gcaggcggca ggaattgccg cggcagacgg gcagccgcac
    11581 gatattggaa agcgtgtgca tgatttttcc cgccgtgtcg gatgactgta cggcatacgg
    11641 aaccatgcag tttgtaattt tatccgtcag ttccccccat gcggattcgg ggtccgtctg
    11701 gcggcggcgc gtttccacat atccgctgaa tctccggcgt cccaggatca ggtaaaaggg
    11761 gacggcaata tatggaaatg ccagcaggga aatgagccac gcgattgttc cctgaggcgt
    11821 acgcgtatgg agcagggccg gaatcaggca gaaggcgcct gcaatgtggc aaaaggcggc
    11881 aaatcccaaa tatggctctt caggaatgat catggaacgg aaaaccggaa aagatgcggg
    11941 ggatgaaaac ggcggggaga agtgaaaagg ctggcttttg gagcaggaga tgatactaag
    12001 gtgacgcatg ctcgtattat cacgggtcca gggattagaa aagcgtaatt taagggaatg
    12061 aagcagtatt tggcactttt ggaggatgtc ctgagcaatg gagcggggcg ggaggaccgt
    12121 accggaacgg gcactatcgg cgtgttcggg cggcagagca ggtatgattt gcgcgacggt
    12181 tttccatgtc ttaccaccaa gaagcttcat ttacgctcca tcatttatga gctgctctgg
    12241 ttcctgaaag gagagaccaa tatcaagttt ttgaaagaca acggcgtcag catctgggat
    12301 gaatgggccg acgaaaacgg ggatctgggg cccgtgtacg gcgcacagtg gcgctgctgg
    12361 ccgggaaacg acggcaaacc cattgaccag attgccagac tggttgacgg cctgaaacac
    12421 aatccatggt ctcggaggca catcgtcagt gcgtggaatg tggccctggt ggatgacatg
    12481 gccctccctc cgtgccattc cctgttccag ttttgcgtga ttcccgccca gccgggagaa
    12541 gaaaagcacg gcctttccct ccagctttac cagcgcagcg cggatctttt tttgggggtt
    12601 cccttcaaca ttgcttccta tgcgctgctg ttgcttatga ctgcccaggt atgcgggtat
    12661 gaagcccggg aattcatcca tacgttcgga gatctgcacc tgtaccggaa ccatctggac
    12721 caggcgaggg aacaactctc ccgcacgccc cgtcctctcc cggtcatgaa gctcaatccg
    12781 gaggtaaaaa ccattgacgg attccattat gaggattttg agctcgtggg gtatgatccc
    12841 ctgccgcaca tcaaggctcc catctccgtt tagaacattc atcccttaat ccatcatgtc
    12901 acaacccgtt acttatacag gagtggtcgc catggctccc gaccgcggca ttggttaccg
    12961 gggggcgctc ccttggcatt tgccggacga cctcaagacg ttcaagcgca ttacgacggg
    13021 gcaccccgtg ctgatgggcc ggaaaacgta tgagagcata ggcaggcctc ttcccggcag
    13081 acagaacatc gtgctgaccc gtgacccggc gtggacggcg gaaggcgttc aggtggttca
    13141 ttccgcaggg gagttggaat gtctggaatt gatggacccg gaaatcatgg tgatcggggg
    13201 ggcggagatt ttttccctga tgatgccggc catgtcccgc atgtgggttt ccaaagtaaa
    13261 gggggaatat ccggcggata cttttcttcc tccgtttgag aaccggctgg agcgtgtcgt
    13321 cctgaaggaa cattttgaag attttgatct gtatttgtac gagcaggtca aatagccgct
    13381 cttgtccgta actccgcggg aggcgctgaa gtataaaaaa gaaaaagacg gggcggccag
    13441 ggccgccccg tccttcatgg agcagttttc ctttacattt attccgcctt tctggcaaac
    13501 aggcctttca cccattcgcg gtaacgctgg aacatggcgt acagggccgg aatgaacaga
    13561 atgcccacca ctgtagcgag aatcatgccg tagaaggtgg agatgccaat ggctttgcgg
    13621 cttgccgcgc ccgctccgga ggcgatgacc atggggaata ccccgatgat gaaggaaatg
    13681 gccgtcatca tcacggcgcg gaaacgctgc cgggcccctt ccagggcggc ttcctggatg
    13741 ggaacgccgc gctcccgctc catcttggag aactccacca tcagaatagc gtttttcccg
    13801 gccaggccca ccagcatcac caggccgagc tgagcgtaaa tgctcagggg cgtgttgcag
    13861 ataatcaggc cgagcaaagc gcccaacagg gcgacggaga cggagacaat gacggaaatg
    13921 ggcaccgtcc agctttcata ttgcgccact aggaacaggt agccgaagag cagggccata
    13981 cccatcagca ggacgatttt cccatcgttc tgccgttcct gatagctcat gtccgtccag
    14041 gtgatggagt aattttccgg cagcggaata gcctcaatct ggttcatcag ctcgccgctg
    14101 ctgacgccgg gttttgcctg ggcggtcact tctgcggaca tgagctggtt gaaacgggat
    14161 atttgccgcg gccccaccat gtaagacagg gtggctacgg agctgagagg caccatctgg
    14221 ccctgatcgt tctgaatgta ggtgttcatg atgtcattga tggtggtgcg gtcctctgcc
    14281 gcggactgca tcttcacctt gaacgtgtag ccgatcaggt tgaaatcatt gatgtacatg
    14341 gaggccagct tgctttgaag cgtcgtgaag atgcggctga cgggcacgtg catgctctgc
    14401 gccttttcac ggtcgatgtt caggaaaagc tggggggtgt tcgcctcata cgcgctgcgg
    14461 gcgtacatgg ttcccggcag tttgttgatt ttgtccagca ggtcgttggt cactcgttcc
    14521 aggtccttgg gagtttcctc cccgctggcc tggagcatga aggagacgcc gcccgtcagg
    14581 cccagcccca tgatggcggg cgggctgaac acggtggcct tggccgccgg aatttcatca
    14641 cacaggatgg aagctttctg catgatggaa tccagggaaa gctccggcgt cttgcgtttg
    14701 tcccaggggt caagctgggc aatgcacatg cccaggtttt ccccgttgcc gcccatgaag
    14761 ctgaatccgg aagtggagga aacctggcgc acgccgggga tgctcatcag cttctgttcc
    14821 gcactgcgca tggcctgttc cgtacggccc agcgtggcgc caggggccag ctggatatca
    14881 cagaaaacgg tgcccttgtc ttccggagga aggaaggatt tgggtacggt ctcaaacagt
    14941 ttccagttgc cgaccaggac agcggccagc agaatcagcg taagccacgc gcggcgcacc
    15001 atgatgccgg cgcatttgat atagcttttg cgcgcccatt ccagagagac attgaagggg
    15061 cggaagaggc tgaacctgct ctttttcttt tgcttccttt tcagcagcag aacgcacagc
    15121 gcggggctga gcgtcaggga attgatggcg gaaaggcaga gggccacgca catggttnnn
    15181 nnnnnnnacg gagaattgca tgtaaatgtt ccctaccatg ccgccgaaga aggcgatggg
    15241 cacgtaaatg gcgaccgtca ccagcgtggt ggcgatgatg gcgcctgtaa tctggtgcat
    15301 gctcttcttg gtggcttctt ccggcgagag atcctccgtc tccagaatgc gcatcgtatt
    15361 ttccactacg atgatgccgt cgtccaccag ggacccgatt accagaataa ggccgaacat
    15421 ggtcagcaca ttgatggaaa agcccagggg cagcaggatg gcgaaggtgc ctatcaggga
    15481 gacgggaatg gcgagcgccg gaatgagcgt ggcacgccag tcctgcagga acaggtacgt
    15541 gatgcccacc accagaatca gggcgatgac aagcgtttcc acgatttccg ccatggtggc
    15601 ggaaatgtat tgcgtggggt cgtagctcac gacgtagctc acaccttccg gaaaacgttt
    15661 ttccagcttc tccagcgtgt ctttcacgcc gttcatggct tccagggcat tggcgtcatc
    15721 caggcggtac acagccatgt tcacggagtc ttccccgttg ttgcggctgc tgcccgtgta
    15781 ggtttccgcg cccagctcaa tgcgggcaat gtcgtccagc cgggtaacgt ggccatcctg
    15841 gcccgtgcgg acgataatct tgctgaattc ctccacggtc tgcagtcgtc cggtgacgtt
    15901 caccttgtat tggatgaagt tgttttcccc ttccgagccg atggagccgg ccgcggcctg
    15961 aacgttctgc gccttgatgg cattggaaac gtcatccggc gtgatattca gggcggacat
    16021 gcgcagggcg tccagccaga tgcgcatgct gtaatttttt tccggcatca ggttgacggc
    16081 ggaaatgccc ggcacgcggg caacctcatc cttcaccctg gtcttgacga agttgttcag
    16141 ctccagggaa ctcatgctgg tggaccggaa agcgaagaat cccaggatgt cagaggagcg
    16201 tttcttgatc ttgataccct ggtccgtcac ctccttggga agtttgtatt ccacccgttt
    16261 caaggcgttc tgaacgttca ccatgttgat gtccgaattc gtgccgctct tgaacgtcag
    16321 ggacagggaa tacccgccgg tgttatccga cttggaggaa aaatagagca ggttttccaa
    16381 cccgttgagt tcttcctcaa tgggggaggc cagcgtttcc atcaccacct gggcgctggc
    16441 acccgtatag gtggcctgca cgttgatgct ggtgggcgcg atttccggat actccgcaat
    16501 ggggagctgg ttcaggcaga tgccgccagc cagcatcatc aggatggcga tgacgatggc
    16561 gaatttgggg cgtttgataa acaggtccgc aatcatgggt agctcagttt tgaacggaat
    16621 ataccggatt caccttggcg ccgggacgca ccttgtgcat gccgtcgatg atgacgactt
    16681 ccccttcctt aacgccggaa ctgaaacact gttcattatt gaccgtgttg cccagcgtaa
    16741 cgtcccggcg ttccacgacg ccctggtcgt tgacgatgta aacgtagctc ttgtaggcat
    16801 cgtgcatcac ggaggaaatg ttggcagccg ggaagcggtc cacgtttttc ttggaaagat
    16861 taaccgtgac tacgccgccg ggcgtcagca cgtcttccgg attggggaat tttgcccaga
    16921 cattcagggt gtccgtggtc gtctggacgg tattgtccgt catgaaaatc ctgcccggct
    16981 ggtcgtatgc cttgccgttg gcgaggttga cggacacaag ggcgtctttc ttcatatttt
    17041 cctgggtacc gaatagggaa aggaaatcgc gttcactgat agggaacttc acgtacactt
    17101 catcgatgcc cgtaatggtc accagggaac cggaggtggg ggtgatgtaa ttgcccgtgg
    17161 aaaaggtgac gcggcctgtg cggcccgtga tgggagccgt gagcctggta tagttaagat
    17221 cgtccttcgc taccgtaatg gcggcgtttg cggactggat ttccgcttcc agggcatgca
    17281 tggtgctctt ggcgttttcc accgtgtcca tggagacgga tttggaggcc agcagtctgt
    17341 tatagcgttc aaaactattt tttgcgtaaa gcagtttggc ttcaagctgg gcctttttgg
    17401 ctaccgcttc ctccacagcc gccctgtagc gcgtatcctc aatttcgaac aggagatccc
    17461 ccttcttcac gacctttcct tcccggaaac gggtggctac gatgttgccg gagacgcggg
    17521 gttgcacagt cacccggtca atggcttcca cctggccgat gtacttcttg ttcaccgcgc
    17581 tgtcaatagc ggcggctttc tggacgagta cggtactggg tttggctgca gcccctcccg
    17641 gcgttccttg ggaataaccc ggaacaaaca gggaaaaaag caggccggaa ccggccagca
    17701 ggcggagggg cgccttccga tgatgcgtgt gttttttcat atggtattat ttactgtaaa
    17761 cgtggttgta aagggtatcc acaatacggc agaaattctc cttgtcctct tgcgacagag
    17821 tgctgaacag ggtatccagt ttttccttca tgccgttgaa aagcatctgg aacctggatt
    17881 cgccttcctc ggaaaggcgg atgcacaggg aacgcctgtc gcggggattc tccttgcggt
    17941 cgaacaggcc tttcttcacc atgctgtcca ccagcaggga ggtggaggga atgctcatgc
    18001 ggagataatg ggcaagctct ttcaggggaa tgccttcctg gcggttggtc atcaggtggt
    18061 tcagggcacc cagggcgttc atctgccgga cggaaagccc catgattttc tggtaatggc
    18121 tctggtccac ctcattcagg ttatcccttt tgaactggtg aaggatcgtc tcgaatttat
    18181 gaacggaggc tgtgatatcc gcggcagtct gaagcatggg ggtgttatgt caaaaccggt
    18241 taattgttgt caaactaaaa caaataaagt tgagaagtta ttgctgtatt gaaataaatc
    18301 gggtaactga aaagttacgg tttcttcacc gtacaaaccg ccgacggttg cagaagttgc
    18361 aaaaaaagtc tttttttctt gtcaccgggg tataaaggcg tgtataagcc tccacgtccc
    18421 cgccgcaagg cagggacggc atccaaggtt atggagcggt agctcagttc ggttagagcg
    18481 ccagcctgtc acgctggagg tcgcgggttc gagccccgtc cgcttcgcca ccttgttcag
    18541 gaaaaacagc cgcctccggg cggctttttt tcattcggac ttttactgtt cctgcattcc
    18601 gtctgctgtt tcaagaaagc cgtccgggag aacagcgttt ttctgaggca ttgggacaga
    18661 atttgctttt acgattcgtt tcccggcgat tttgccttgt cgcaacagca gcaaagagta
    18721 ccgcatccct tccggtttgc gggaaaatgc ccgttttccc tgcccgttgg agaattgctt
    18781 tcctgcaggg cgggggagcg tgcttgattt aggcgcaatc ttccctcatg atgtcggcat
    18841 gattctggaa ctgcactccc cgctggacat gcatcttcac ctgagggacg gcgatatgat
    18901 gaagctggtc gctccgttaa gttccgcctc ttttgccggg gcggtcatca tgcccaacct
    18961 ggtgcctccg gtggctgatg cgggtgcggt gcaggcttac cggcagcggg tgctggacgc
    19021 ttgcggagac gatgtattcc agccgtacat gacggcattt tttcgctcct attcagaaaa
    19081 ggaattgtcc cggctcaagg aactggtgtt cggcatcaag ctgtacccgg caggagccac
    19141 cacgaacagc gagggcggcg tgaaggccat gaaggatgcg gaagctaccc tgtccatcat
    19201 gcaggaaatg gatattcctt tgctggtgca tggcgaaagc cacggcttcg tgatggaccg
    19261 ggaggccgaa ttcctggatg tttaccgtga tttggctacg cgcttccccc ggctgactat
    19321 ctgcatggaa catattacca cggccgccgc cgtgcagctg ctggacgaat ttgaaaacct
    19381 ggccgccacg gtaaccctcc agcatcttct cattactttg gacgatgtgg ccggtggcat
    19441 gctgaggccg catctgttct gcaagccgat cgccaaaagg ccggaagacc gggaagccct
    19501 gttgcaggct gccctttccg ggcatccccg cctcatgttc ggcagtgact ccgcccccca
    19561 tcccatccat gccaaggaag cgtgcggatg cgccgccggc gtatttaccg cccccatcgc
    19621 tcttcctcgt ctggcggccc tgtttgacga acacggggcc ctggaccggt tgcagggctt
    19681 tgtttccggt catgcctgcg ctttgtacgg gttgaatccg cctgccagga cggtccgtct
    19741 gcagcggcgt gaaatgctgg tgccggacgc ttatgaagga catggacaga aagtggtgcc
    19801 gatggatgcc ggatgcacca ttccttggag actgatatga ggggcttttc caggggaaga
    19861 tgacagaaag ctatttaagg gaaccgtctt tatactggat attccgggca atctcactac
    19921 aatccctgcc catggatcgg attcgcgttt atacgggtgg agttgcttcc tgtaatggtt
    19981 acctctttaa gacgagggat aacgcttacg tggcggtgga cgctccgtcc ggctttgcgg
    20041 actggatcta ttccaaaaag cccgatatcg tcatcacgga tcttctcatc acccaccagc
    20101 attttgacca tgtggaggat gcctgccgca tgcgccagat tttcgactgc cgcattcatg
    20161 ccgggcaatc ttacaatgaa agcctgacgc tggaaaaaat ggcccgcgac gcctgggggc
    20221 ttcccctcaa tgttcagccg tttgtggtgg atgaaatact tactccggac attcacacgg
    20281 cgaattgggg cggcctgttg tggcatctcc accaggtgcc cggccattct ccggacagca
    20341 ttgtttacga tttgccggat gaaggaatca tgttcacagg tgacgtcatt tttgccggct
    20401 ccatcgggcg gacggatctc ccgggaggaa acctgcgcct gctgaaacag ggcattgaaa
    20461 agaaagtgct caaccagccc gccacgacca tgattttccc gggccacggt ccatatacca
    20521 acgtgaaaaa cgaactgctg accaatccgt tcatctcctg accgcgattc ccgcgccgct
    20581 gtacggaatc gtgttctact ctttagcaag catacaagat caatgttatt ttcagaatta
    20641 ggtttatcgg aacccgtctt gaaggcggtg gagaaatgcg gttatgaaca tcccaccccc
    20701 attcaggagc aggccattcc catcattctg gaaggcaggg acctcattgg ggcctcccag
    20761 acggggacgg ggaaaaccgc tgctttcgcc ctcccgctgc tgacaaggat tcagcccatc
    20821 ggcaaacctc agatactggt gctggaaccc accagggaac tggccgacca ggtggcggaa
    20881 tcctttgccg aatacggtga attcaccggg ttgaaagtag cgttgctgta tggcggcgtg
    20941 gggtacggaa agcagacgga agacctgaaa aaaggggcgg acatcgttgt ggccactccc
    21001 ggccggctgg tggaccactt ctaccgctgc accatgcgct tcggagaagt caaggccctg
    21061 gttctggatg aagtggaccg aatgctggac atggggttcc tgcccattgt ccgtaaaatc
    21121 gtcaaccttt gtccgtggga aggaaggcaa accctcttct tctccgccac catgcctccg
    21181 gtcatcgcgg gatttgccaa atggtgcctg acggaccctg cggaagttac catcgcccgg
    21241 cgtgaagtgg ccgccaccat cagccatgcc ttttatccgg tagctctgga ccagcgggat
    21301 gaactgctgt tggccctgct caaggggacg gacttccgtt ccgtcatgat ttttacccgc
    21361 acccgcaagg aggcggacgc ggtatgcggc atgctcaagc atcatggcta ccgcggggag
    21421 gtggccgtca tgcactccga cattccccag aaggaacgca tggaggcgct taagggattc
    21481 aagagcggaa aatatgatat tctggtggct acggatgtgg cggcgcgcgg cattgacatc
    21541 agcggcgtga cccacgtcat caactaccgc gttccggaaa acgcggaaga ctatgtgcac
    21601 cgcatcggcc gtaccggccg cgcggaagct tccggggatg cgttcacgat catgacggcg
    21661 gatgagctgg attttgctgc ggctgtggaa aatttcattg ggaaacccat tgaacgcaaa
    21721 aaactggacg ggttcaacta cacgtacacc gccctgttgg aagacaagcc cgtcaaatcc
    21781 gtccgcaagc ccaaacccgc aggtcccaag cgccgcaggc gctaaggagg gggggggaaa
    21841 catgatgttt ctctctgatg gaatgaagat catcccatgc gcattcttgc catagacccg
    21901 gccatccgca acacgggtta tgccgtggtg gagggggatt accggcgcgc gcgcgccctg
    21961 gactatggaa cgctatccat tccccggagc gtgtcccagt ccggctgcct gcttgccatc
    22021 aagcagcatt tgggcaatct cattgacaaa tggaacccgg atgaaatggc ggtggagcgc
    22081 atcatctatg tccagtctca tcagacggcc atcacgatgg gtgcggccaa ggcggccgtg
    22141 gtcattgccg cggcggaagc cgggctgcgt attatggaat attcccccaa aagcgtgaag
    22201 ctctccgtcg tagggcgcgg ggccgctcaa aaaacgcagg tcgccttcat ggtgcgggct
    22261 cttttggaac tccgggaaac gccggaatcc gatgccgccg acgccctggc gatcggcctc
    22321 acccacctct tttctgcgga ccctttgaag gcccacatga tggagaggaa atacatttag
    22381 tagtgatgag atatgagtgt aaaaaccgta cttgcagccc gcggattgaa atctgaccgc
    22441 ccggcgctgt tctctaatca ctgattcaag ccgtcagggc ctgaacgtcc tcccttttcc
    22501 gtcctgccac gccttgagaa gggaagcata cattttgttg agctgctcca gggtagctac
    22561 acggaattcc acggtattcg tattcgggta agcttttgcg acggttgtat ccgggtcaat
    22621 gctgcctccc gttgcccggt tgacggcgcc ggccccaagc tccttcgcac ggtcagtcac
    22681 attcttgatg gcgttgatac cggaattttg acccagcgtt tccagataat aaaacttggt
    22741 gggcgtattc cccagggtaa tcagcgttac ttcatacacc atgcaggcat tgtccagaat
    22801 atacctgtgc tgcgccaccg ctacaatccg gctgagatgc accacatact gcccctccgg
    22861 aaaattgcct atccagaact ggctctggtt gtagtcatcc gtggacggga cggcggctcc
    22921 tttcttggac tgggcgggaa gaggtgcggc tgcacaaaca aatagagcta aaacaggaag
    22981 gcagaatcgt ttcatgcttt ctatttaata tcagctctta ctgtatggga aggaaaaatc
    23041 gttgaatgtt tgcgggggag aacatgtctg ttcatggata aatgtcatcc ctttttcagt
    23101 tgctcccttg cgccggaata catgactgtt tgaataatat ttactggcac agttcatgat
    23161 attgatagaa aatgccaata ctcctgtttc cggatgaaaa acctgaaacg tacgtaaaaa
    23221 ggtaatggaa tcagggtgtt atggcatgga aatttccatg gaacttatcc atgcatccga
    23281 gcaattccct gaatttatcc gtattgcctt caattttaat catgcctttg tccagagctg
    23341 tctttaaggg cattgcccgc atggcgacca gggcgaagtg gagcctgtct ccgatgatga
    23401 ctgcgtcggg atgtttgacc agttttcctt cgcggtacat cagcacttcg ttttcaatcc
    23461 agaagccgta tgatcttccg ttttgggcaa tccagttgaa agccagtttt ttccctttgg
    23521 atttctcact attcaggctg acgcccaaat agtcgaacag cagttccgga gtcagggcat
    23581 ataaagtgtc cgtcaacgta agttcgcggg agtttttaag cagcccgatt cccggttctc
    23641 cggcattgac gagaagttca tcgttccgga atacgggttc cgtttgataa ccgaactgtt
    23701 tcaacatatt tcttaccaga ttccttatgt ttcctctttt tagatgggca gacataacgt
    23761 tccttaatgt cccggcgtct tgacgatagt ccttttgccc ggtgatttcc ggagggctat
    23821 tcctgattct gccgctttcc ggaatcggat tgattattcc ctcaatttct gcgggggaat
    23881 accctttgct aaccaggttc aatatccggt catgcatgta tttgcagttg cggcattccc
    23941 tggccaggaa ccgtttgata ttttcttttc ctattctggg ccaatggtgt tgtgagaaca
    24001 aaatctctat tttgtcttca taacgctgaa tggcttgatc aagggctttc cacctgtttt
    24061 ttgcatccct tattctggat actcccagag tgcagatatc atgtatggcg cttgccgtgt
    24121 cctctccata aaaaagcgcc ttgaattggg gaaaatacat cagtgcggca gaagaggccc
    24181 ccattccggg aaccgccatg aattccattt ctatgccgtc tatgtccagc ccggatgccg
    24241 ttcttttggt actggcaaaa tagttggtat ggggatgtgc atctacaaca gccttgattg
    24301 gccgttttcc cgccttccgg taatagaggt caagactctc ggccatggtt tctttcggga
    24361 cgaaaggggc aatgatcatg atgccttccc gtccttcaat aatagttatg ttcggaaaac
    24421 ctccgccgtg aatctggtaa attccttttg tgacttcaga cagcgcgttt ttggcattgg
    24481 gccgggaaat gctccacgta accggtttgt ccccggggcc ttgttccgca tcttgaatgc
    24541 cttgacggtc attttgattc gcatcgtaaa ctccgggaga ttttttgttg tcttcaacgg
    24601 gatgccttag cgcctttttg gattgttttg ccataccgac gcctgtaccg ccccatgcaa
    24661 atattgccag aagcgtggct gtcaaatagt tcatttttgt tttcatgatc ggagttttat
    24721 taacggaacg cgtgaaaagt aaaaggaagg ccagttccca tattttactt tgcaggaaca
    24781 atagacttgc caataaagaa caatgttcga aaaatggcag tatgttaata tctgattact
    24841 aaagttttta gctgattggc cggaattccc gccgcaaacg ggaagttttt cgttttttta
    24901 taccgcttat tcttctggtg gaaggcggtt gatattcgga gtgccttgaa gtagggggga
    24961 aatctgtgaa aggatttttt tgcccgtttt caacgggttt atggaaggaa catggctgga
    25021 aatgcggaaa agggtatgtt aatgaacagg gcatggaaag attgtatcgt ccgaatgttg
    25081 cggggatgat ggtccggcag gacgggaaat tgttgatttg cgagcgttcc gggcagaaag
    25141 gagcctggca gtttccccag ggcggaattg acccggggga aacggctttg gaagccgtgc
    25201 ggcgcgagat tggggaggaa gtggggtttt tgccgtccca gtatgatatt gtagaatccc
    25261 ggaaggggta tcgttacgat tatccgccgg aggtgctgga gtatgttcgt gaaaagcggc
    25321 ggcagccttt tgttgggcag gcgcaggagt atttcctgtg ccggctgcat gcggacgctc
    25381 cggaacccgt cctggatgac cgggagtttt gcgactacaa gtggatagcc ccagccgaat
    25441 ttaagctgga gtggctgccg gagtttaaaa agaaagttta cgccagggtc ctggaagatt
    25501 tctttaatgt ccgggcacgg gataagtgaa gtggctcagc aggtcatgtg cggcggaaac
    25561 ggggtccatt ttgtcccccg ccaccatgtt ttccagaatg ggcatgcggg acgctacggc
    25621 ggggtgctgt tcaaaagcgg tgagcagggc ttcatgcacc agggactgga cccatgaaag
    25681 ggactggctc cggcggcggc tgtaccagaa tccggattcc ttcatgctgt cccggaagcg
    25741 gaagagcgtc tcttcaatgg tgtccaggcc gcgtccctcc agggaggaac aggtgaggac
    25801 ggagggcgtc cagccgggag tggggctttg caggtagtgg agtaccattt tcagttcctg
    25861 gcagtgggct gcggcgcgct ggcggttgtc gccgtcatct ttggtaacta ccaggatatc
    25921 cgccagttcc atgatgcccc gcttgatgcc ctggagatcg tccccggctc cggtgatgag
    25981 caggagcatg aaaatgtcca ccatggagcg caccgtagtt tccgactggc cgactcccac
    26041 ggtttcaatg agaataatgt caaagcccgc agcttcgcat gcaatcatgg tttcacgcgt
    26101 tttccgggct acgccgccca aagagccgcc ggagggggaa gggcggatga aggcgttttc
    26161 ctctccggag agttcctcca tgcgtgtttt gtctcccata atggaaccgc gggagactga
    26221 agaagacggg tcaatagcca gcacagccac cttgaaccct tttttgcaca ggtaagtgcc
    26281 gaaggcttca atgaaagagg atttcccggc gcccggaacg cccgtaatgc cgatgcggac
    26341 ggcgttgccc gaatggggaa ggagcctgga gatgagggcg cgggaagatt cctggtcccg
    26401 gacggcattg ctttctatca gtgtaatggc ccttcccagc agggcgcggt ttccggccag
    26461 tactccctgg gccagttctt ctacggaagg gcggtgcgga cgtgcaaatt tgatgttgct
    26521 catgggaaaa gagagtgcgg attatttgaa aggcggttcg ttttctttgg tgcgctccat
    26581 atcataattg aagcgtttga ggatgaattg acggtggtcg gcca
//
LOCUS       DAWEMX010000019        25578 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_782_length_25578_cov_12.226384, whole genome shotgun sequence.
ACCESSION   DAWEMX010000019 DAWEMX010000000
VERSION     DAWEMX010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 25578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25578
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_25578_cov_12.226384"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11054..11063
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15255..15264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19118..19127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5999 a         7639 c         6573 g         5337 t
ORIGIN      
        1 acacagcaga acgcaccgga ccaaggtgca gacctgcgga agcgttaccg tgcaggccaa
       61 tgcagacctg aaggggctga acatcgaatg ccgtgacctg gtcttatacg gcagggcttc
      121 cggcgacttc ctgtgccgcg gcgtttgcaa aatcaaaacg gaccagcaca tctccggctc
      181 tatctgcgcc cacaggatgg tggtggaaaa aaagacgacg gtgctggtca caggaaccat
      241 ccgcgtggaa aacatctgga tacaggggtc tctggaagga acgcttacgg cggatgaaac
      301 cgtcaccatc caccggcacg ccaaattcct gggagatatc acggcgcgcc gcctcatcat
      361 tgaggaaggc ggcacgcacc agggagcctt tacccgcctt acgtgacatg aatcttccca
      421 tttccatccg cggcgcgcgc cagcacaacc tccggaacct ggatctggac cttccgtcca
      481 acaagctcat cgtattctgc ggcccttccg gctccggaaa atcttccctg gcgttcgata
      541 cgcttttttc cgaatcccgc aggcgttttc tggactgcct gtcggcacgc tccaggcagg
      601 gcatggatca accggaaaaa ccggaagtgg acagcattac cgggctgccc ccggccctgt
      661 gcctggagca atccgccagg cagcagagtt cccgcaccct gctggggagc atcacggaaa
      721 ttctggacta cctgcgcatc ctttacgcgg ctgccggcac gccccatgac ccggaaacag
      781 gaaaggaact ggaacgcaag agcccggacc ggattacaga ggaactcgtt tccctgccgg
      841 aacacacgcg cctgattctg gccgctccgg cggaaaacct gctggcccag gatcccgcag
      901 cgacgctggg cgacttccag cggcagggct tcctccgggt ttactggaac ggagaaatgc
      961 gggatattga agaaataagt tcccccgtcc ccccgcctcc ggacgcggcc ctggtcattg
     1021 accgcctcat cgtcagaggg gaaaatacgg cctcgcgcat tgcggattcc ctgcaaacgg
     1081 ctctccgtat caatccggac gaagtgcggg ccatcatcac cataccggga gaggaagcct
     1141 caatccgggc cttccacacc cgctaccgca atccggaaac aggcttcctt ctaccccagc
     1201 ttacgccccg ccatttttcc ttcaactccc cgctgggggc atgcccctcc tgccggggaa
     1261 ccggcctgaa tgaacaggaa aacggtccgt gccgcgcctg cggaggccag cgtctttccc
     1321 ctctggccct ggccgtcacc atgcctgcgc cggaccgggc ctacaatctc gcggaactga
     1381 cggctcttcc tctggaagat atggcaggag aactggaacg actgaaaacg ccctcctccc
     1441 tggcggcggc attgaccccg ctcatggagg aaatcaacaa acgcgtgcgc ttcctgaatg
     1501 agctgggact ctcctacctg tccctggacc gccaggcaaa caccctctcc ggaggcgaac
     1561 tgcagagggc gcgcctggct tcccagctgg gaggcggcct ttccggagtc ctttacatcc
     1621 tggacgaacc cacggccgga ctgcaccccg ccgatacgga ccgcctgctc cgcgctctcc
     1681 ggacgctccg gaaccagggc aacacagtac tggtcgtaga gcatgatgaa caaattctaa
     1741 ccgcggcgga tcacctggtg gacatgggcc ccggctccgg aaccaacgga ggccgtattc
     1801 tggcacaggg ctcccttgcg gagatactgg aaaatgccga aagccccacc ggagaatggc
     1861 tttcaggcaa gcgaaatatg cccgcctccg gacgcaagac ggctcctgcg gggcgtctgg
     1921 tactgaccgg tgcggacaag cacaacctca acaacgtcac tctgaatatc ccggtcggca
     1981 cactaacctg catctccggc ccttccggtt caggaaaatc cacccttgtc cgggactgcc
     2041 tcatccccgc agtcaggcaa gacctctccg ggaaaaaggg tattccgcgc cgcgtgcagg
     2101 gaacggaaca cttcaaccgc ctcgtcgtca tcgaccagtc gcccatcggc aaaacgccgc
     2161 gctccacacc ggccaccgct accggcctgc tccaggtgct gcgccccctt tacgcacagc
     2221 tccccctttc caagcagagg ggatatacgg cggcgcgctt ttcccccaac attcgcggag
     2281 gccgctgtga acgttgccag ggaacgggca tgattgaagt ggacatgaac tttctgggaa
     2341 acgtggcaat gccctgcgac gcctgccagg ggcagtgcta caacagggaa acgctggaag
     2401 tcacctggaa agggaaatcc attgcccagg cgctggccct gaccgtggac gaagcggcgg
     2461 aattcttttc ctccctgccc agagccgccg ccatcctgaa aagcatgcag gacgtagggc
     2521 tgggatacct cagtctcaac cgcagggcgg acaccctttc cggcggagaa tcccagcgca
     2581 taaaaatagc tgcggaactg gccaaagccc cggcctggaa actggaggaa gacggaaaac
     2641 gggccctgtt cattctggac gaacccacca gcggcctcca cttcaatgaa gtggcccttc
     2701 tcctggcagc ccttttccgc ctgagggatg ccggacacac catcctctgc gtggaacacc
     2761 acaaggacct gctcaatgcc gcggactacc tggtggacat gggccccgga gccggcaggc
     2821 acggcggcaa tatcgtggcc gagggctccc ccgcagatgt agcgtccaat ccggaagcgc
     2881 ccacttctcc ctggctcgtc ccccgttaac aacggcagcc cctgaaaatc attcatctgt
     2941 cctgtccccc ggacatcccg ttcccggcgt cttcggaaaa aaatctgcct ctccggggtt
     3001 tattcgcctc cttcccctat ttattatctt gacgaccctc ccgggctttt tttaacaaaa
     3061 aaggaattac acatattcct tttttcctat gactctccgc tccctctctc cggctctggc
     3121 ggcactgctc tttcccggag cgcttcatgc cgcgcccgta ttccttgaaa acgtctccga
     3181 atccggcgga tggtatgatt gcaataaaaa aaccaaatgg acatggggga ccaagcccca
     3241 ccccaccaat ccgctaaatc ctccggtttc cgcacggccc aatgaatggc tgtaccttcc
     3301 ttatgatcag aatatctgct gggcggcggc ggcctccaac gttctccaat ggtggcagga
     3361 taccagaagc gacctgaaat ccgccacgcc caacgggaaa tccgccactt acgatgtcat
     3421 gccgcaggtc gcccagctgg ctatttacca gaccatctcc acgaactgga caaacagcgg
     3481 aggctccgtg gaacaagcct acaactggtg gatcaacggc ggaatgctgg ataccaccgc
     3541 ctatccgagc ctttctacga cgacggcttc cggaggatac tggaaagagc tgggactgac
     3601 agtcccgcag tcttcaagcg gaggagctgt tgacaacccc ttattttccg catacagctt
     3661 ctgggacgga gacaccagaa gcggcgttta ccagaagctg acaaacagca tcaacaacaa
     3721 ctggggaacc actctgaccg tcggcgaaga agggagaggc cacgccatca ccatgtgggg
     3781 ctacgacacg gacgaagacg acaatctggt tatctacctg acggactcgg acgactatgc
     3841 ggtgggaatg ttccgccaga aagtagtcgt gaacgactat acggatgaat acggcctttc
     3901 ccactgcgac atttatctga ccagcatgga tggagaaaac gacgtgtaca gttataatta
     3961 cgaagaagcg ggactgacgg gcatccgcct gggggaaata cagagcttca ccgctccgct
     4021 gggagatctg agaatcccgg aacccggcgc cggaatccta ttccttggcg gttcattcct
     4081 cgccctgtcc cgccgcaggc gcgctggacg gtaaagcttt ccgaactccg gccccaataa
     4141 aaacgggtct tgattcttat gggggaaatg tgcagaatcg gcgagcgcac aagttcagga
     4201 gagcaaaaat tgtcccagtg ttccccagtg cggttacctg tgaaatgaat gacgcttccc
     4261 acaaccacgg cgagcaggaa aacaccgtct ccacctattc cgtcgacgta gaaacccttc
     4321 agcaaatagc tctgaaagga gacgcccagg cgctgttcca gctggcaatc aactacgaac
     4381 agggtcgcgg ggtggcggaa aaccagcagg aagccttcta ctgctaccag caggcggcgg
     4441 aactgggcca cgttacagcc cagctcaacc tgggatgggc ttattccaac ggcatcggcg
     4501 cccctcagga caacgacaag gccttttact ggtaccggaa agccgccgaa caggggcacc
     4561 ccaccgcgca atttgacctt gggttctgct atgtcaatgg cctgggagtg gaaaaagacg
     4621 aacaccaggc catcggctgg tacaaaaaag cggcggaaca gggccacgcg gtagcccagc
     4681 tcaacctcgg ctggatttat gccaacagcc ccagccggaa aaactgggaa caggccgtat
     4741 actggtacaa gcaggccgcg gaacagggag acccccgcgc ccagtataac ctggcctggt
     4801 gctatggcaa cggaagcgga acccccaaaa acccgcggaa agccgcttac tggtacgaag
     4861 aagccgccat gcagaaccat gctacggccc agtacaatct gggatggtgc tatgaaaacg
     4921 gtttcggcgt ggagccggat ctggacaaag cgctggtatg gtatcacaaa tcagcccttc
     4981 agggccagat tacggcccaa tacacgctgg gatggtgcta cggcaacggc cggggcatgg
     5041 aagtggacat ggccaaagcc gtccactggt acaccaaagc cgcagaacag gggcatacaa
     5101 cagcccagct caacctgggg tggtgccatc tgaacggaaa aggaacgccc gtcaaccggg
     5161 aagaagccct gaaatggtac cttaaggcgg cggaacaggg caacgccaca gccatgttca
     5221 atgtgggcaa ctgctacgcc cacggctacg gcattgagca ggacgataaa caggcggaag
     5281 aatggtatca gaaagccgtc cggcacggca acaaaaaggc cgccagcgcc ctgcgccatc
     5341 tggcctccaa acaggaaaaa gagaaacaaa cggatgaaga aacgtagatt cttttatgaa
     5401 tgaaaataaa tgtgttaaaa taaaaaatat tttgaataat ttttattttt taataatttc
     5461 tagaacctac atctacagcg gtgctccttt aaggagtctt tatccgggag aattttggaa
     5521 caattgattt ttttgccacc acatggcaaa atatattctt taaatatttt actattctga
     5581 atttctgatt tttggctagg cattacacaa tattcatcat ctaatgcaaa gtatccgttt
     5641 tcaaaagctt tatcgtgtaa aacacataag cacagcccat tagagacgtt accacgtttt
     5701 tcagaattat cgacccatcg cgcaatatga gaaccaatta aaaatttggg atcatccatt
     5761 tcacaatcag gaaaacagca tttatatcca taatttttct taacgccctc agaaaattga
     5821 atttgtccaa tgcgttgttt tacactcctc catacttcag atgcaggagt tgcttttttt
     5881 acaccacttg aagtatctgg ctcattattt tcccttaaga ttaaggatag taattttttg
     5941 tcacacttgg aaagatatgc accattcaaa cattgtaacc tcccgttttg atgtacaaaa
     6001 aatcgattga ttggtatttc agtaagctct ttgagatatt ttatcaactc atttttatgg
     6061 atagaaaaaa aatcgtctaa aagaataggt gttttgaaag ggtggtagtc tttaagcaat
     6121 acacgaaaat aactggtcgc ataattccat tttccccctt gaggaggcct ttccaatgtt
     6181 tcatgaccat cagtagcagc tatagaatat ccaacaaatg aggctaaatg ttttttacct
     6241 cgcaaatgta aaacaatatc gccttgccgg acgttcaata tattattcca ataaagccaa
     6301 gaaatttttc gacttttatt cgagcagaat ttgtatgtag gggaccagag gcatttagta
     6361 aatccccact cacctccacc atgaatatta tctctgctca tttcaaccca aatcataata
     6421 tactgtctta tcacatattc tttattatat caatatccta actttaattc atttatattt
     6481 caaaatttag caaaaaaaat aacataacta ttttaatctt aaaatttatc gttttcccca
     6541 atttctagtt ctaccaaatc ttgacagccg ttccacggcc tccggcagcc gggctgcgcc
     6601 atcgccgccc agcgctgcgt caatatcttc cagcgcatgg atcaggtcaa tgacctcccg
     6661 gcggggcaag gcgcccccgg ccggtacgca gaaatgagtg cggcacgcca acggcctgcc
     6721 ttcataaatc atgcatcttc cctgaccgct caagaaaggg cagctcccgt ccggaggcag
     6781 ctccaaccgg gtgcgtccgg aagcacgcca cgccttccag gccacccatg cctcccccag
     6841 cgttacatgc ggcgtctccc cggtcaggcg gaaccggcag caatccgccg caccggtaca
     6901 gaaacgctgg gcacgggaag cgcggaaggc gccttcatcc agaaggcgcc gcacctccat
     6961 cagcatcccg gcggcggggg ccattccccc cgcctttccc tggcggcatt tgccacgcgc
     7021 catgacagaa acggttaaca ggtcaggccg tccactttcg cctggttcca gtatgcatac
     7081 tcggaacggt tgaaatccac atattccgga gacaaatccg tggtatacac cgtatgggaa
     7141 aactcgcccc gattcaaatt gatatccacc tggaacgcgg gaacctgcac ggcttgccgc
     7201 aaatcgtcgg acggagtatc cgcctgcacg ccgccgcggc aggcggccag accggcgatg
     7261 tcaatgtcta tcttctcttc gtccaccttt gctccgcagt agcctacggc atgaataatg
     7321 cgcccccagt tgggatcatt cccgttccag gaactcttta ccagggaaga cttggctacg
     7381 gcttccgccg cttttttcgc ttcatcctcc gtgcggcctc ccgtcacatg cacggtgacg
     7441 aacttggtca cccgctctcc gtcctgcacg atgtgcttgg ccagttccag catcacatgg
     7501 gccaaagcct gctggaatgc gtaaatatct tccggagttt ccagcttcac gccggatgcc
     7561 ccattcgcca tcactagaac cgtgtcattc gtgctcatgt ctccgtcaat ggtgatgcag
     7621 ttaaaagaac tcttcactcc ggactgcacg cacagctcca gcacatcgcg ggaaacgccc
     7681 gcatccgtcg taataaagca cagcatggtc gccatgcagg gattgatcat gcccgccccc
     7741 ttgcagcagg cgccgatgcg cacgggtttg ccctgcacta caaactcaat ggcaataatt
     7801 ttctccttcg tgtcactggt catcacggcc tgggccacct catgcccctt gtcgcgggaa
     7861 agcccctccg ccaattccgg aaacttcggg taaatgcgca tcataggcat tggcaggcca
     7921 atcacgccgg tggaacatac ggccacttct gcaggctcca gccccagaag ctcagcaacg
     7981 ctgcggcatt cgcccctggc gtcctccacc ccccgggttc cggtgcaggc gttcgcgttc
     8041 ccgctgttcg ccacgatggc gcggatattt cctttccgga gatgctcccg gctcaccttc
     8101 acgcaggcgg cctgcacgcg gttggtcgta aacgttcctg cggagacgca gggttccgtg
     8161 gaataaatca gggccaggtc cagacgcgtg gccgtaggct tcttgatgcc acagcttacg
     8221 gcgctcccca aaaagccctg gggcgcgcaa acgcccccgt caaccggaat ataggatgaa
     8281 tcattcatcg tcttgaaagt gcctttcagc gtatcaaaaa gctacagaac ccacaagcct
     8341 tccgtttcat caaatccgca catgatattg aaggactgaa cggcctgccc acccgcgcct
     8401 ttcaccacat tatcctctgc gctcatcaga atcacgcggt tcgtgcgggg atcataagcc
     8461 cagccaatat ccacgcagtt cgtccgtgtt acattcttgg tatccgcagg ctggttacgc
     8521 cccagaaggc ggacgaacgg ggccgcggca taggcctctt ccagcaggcg gccgacggac
     8581 tcggggtccg cccctttttt cactttggcc gtaatcgtgg agcaaatacc cgtattaacg
     8641 ggaatcaaat ggggggtgaa ggacatcacc accgtttccc cggcggcatg ggaaagctcc
     8701 tgctcaattt cgctcaaatg gcggtgtttc ggtactccgt aggcatgaaa actctcattg
     8761 cactcgcaga acagcagcgg aatggatgcc ttgcgccctg ctccgctcac gccgctgccg
     8821 gaacagacca ccacgtcctc cggctccagc atgcccgccc tgaacaaggg aatgagaggc
     8881 agaagaatgc tggtgggata gcagccgggg gaagccacaa tgcgcgcccg ggcaatctcc
     8941 tcccggcgcc actccggcaa accgtacacg gcttcctgca tcagggccgt atccgggtgg
     9001 gggttcccgt aatactcttc gtacacctcc gggctgtcca ggcgaaaatc cgcgctcagg
     9061 tcaatcacgc gcacgcccct gtccaccagg ccgcgggcat aggaagcagc cacgccatga
     9121 ggcagggcca gaaaagcgac ctccgctcca gtggcggcaa tcgcctccac atcggaatcc
     9181 gtaaacttca ggccggaacc cggaacctga cggaaacggg gaaaaacctc ccacaacggc
     9241 tgaccggcat actggcggga agtagcgcac accagcttca ccccgcgatg gttcagcagg
     9301 atgcgcaaca attcctgccc ggtgtagcca ctggccccga cgactgcaac ttgaacttgc
     9361 ttcatgacaa aaaaggtatg ctatgaaaaa agactcttgc caacactaaa gttttcctat
     9421 atacacctcg ccgtcacaag acacgaccct ggtcggtccg agttcatcgg gttgtagcgc
     9481 agtctggtag cgcacctgca tggggtgcag ggggtcgcag gttcgaatcc tgtcaacccg
     9541 accaatttcc cattcgtgag gccttgcaga atacttcatc ctgcaaggcc tcaaactttt
     9601 ttgctttgcg cggtattcca cccagcccta tggttccgcc atgcaccttg ccatgcatca
     9661 cattccggat gcatgacaat tttccaaaac caaacataca ccgccaccaa agcaggaagt
     9721 ttgatcctcc ctccctttgg aaatattaat cgggaaaatt atcacaagat acattccctc
     9781 cctcttgcta ataaacaaaa aaatcctggg tatcttccgg gttcatgcca ttcatattcg
     9841 ttgccatttc ctctccgtcc ggcccgctac tccgcttccc tgccgtggga caggacaaac
     9901 cggaaccggg aggaaacagt tttttattgc gttgcaggca ggtaaatcat ataccccgcc
     9961 ggttcagact tcaccttttc ccccttgctt tcatgccatc catttccttc ctgaatcatc
    10021 tcggagccct gctgacgctc tggtttctcc tatgctgcgg cgcaccagcg gcggaaaacg
    10081 gcagaatcct gatggtgacg gaagcgactt tccctcccta cgaattccgg gacggaaacg
    10141 ccatcctggg cattgacccg gaaatcatgc gggaggtagc ccggcgcacc ggcagggagc
    10201 tggtcattga agacatgagc tttgattccg tgattacggc tgtagtttcc ggaaaggcgg
    10261 acgtagccgc ctctggaatc acggttacgc cggaacgcag gaggaaagtg gacttctcca
    10321 ttccctatgt ggaagccgct caggttatca tcgttcccaa aggttctccc atccggaagc
    10381 gggaagatat ccgcggcaaa agaatcggag tgcagcacgg ggccacggga gacatttacg
    10441 tcacacggaa catccagcag ccggaacgct tccctaacgg agcgctggct gtggccgccg
    10501 tggcggcagg caaggtggac gccgccgtca ttgaccagga tccggcaaca atgtacgtag
    10561 cgggcaatga tcggctggaa attctgccgg agcccctcac ttccgaatcc tacgccattg
    10621 ccgtccgcaa gggagatacg gctctccttc aggatattaa cggcaccatt gcagcattga
    10681 aagcctccgg aaagctggag gaaatacgga acgcttatgc ggccatgcag gttaaatccg
    10741 ctgccggtcc cgaacaacac gcttccggag ggggctggct ggaaaatata tggctggacc
    10801 tgaaagaatc cttttatgtc aatttcatgc aggaaggccg ctggaaatac ctgagcaacg
    10861 gctttctggt gacggtggag gtctctttct tttccgttct gctgggcatt ctggttggct
    10921 ttgtggtggc cgtcatccgc gccacgcatg acaaaacggg caatctccgt ttcctgaatg
    10981 ccctctgcaa actgtacctg acagtcatcc gcggcacgcc ggtggtcgtt caactgctca
    11041 tgatctattt tgtnnnnnnn nnnccgtgga tgtcagcaca gtgttggtgg ccgtggtggc
    11101 cttcggcttc aactcaggcg cctatgtggc ggaaatcatc agaggcggca tcatgtctat
    11161 tgacaaagga caatttgaag ccgggcgcag cctcggcctg ggatatgccc aaaccatgat
    11221 ttacattatc ctgccccagg cctttaaaaa tgtacttccc tccctgggaa acgaatttat
    11281 cgtactgttg aaggagacca gcgtctccgg ctatattgcc ctccaggacc tgaccaaggg
    11341 cggcgacatc atccggagcc agacttatac cgccttcatg ccgctgacgg ccgtggccct
    11401 gatttacctc tccgtggtca tgctgttcag ctggatgctc ggcaaactgg aaagaaaact
    11461 gaaaaacaat gaatgaacat cccggaaaca ctcccatgtt ccaaatcagc catttggtta
    11521 aggaattcaa ctccgtgccg gcagtcaacg atgtcaccac gaacattgac cggggtgaag
    11581 tggtcttcat cgtaggcccg tccggttccg gtaaaagcac cttcctgcgc tgcctgaacc
    11641 tgctggagga accgacctcc ggagaaatcc gtttcaaggg aaagctggtt acctctcccg
    11701 gaacggacgt caatcaattc cgccagcacg taggcatggt tttccagcat ttcaaccttt
    11761 tcccgcacct gaccattctg gaaaatatca ccatcgcccc ggttaaaacg ggacgctcca
    11821 gcagaaagga ggcagcagac caggcggaag cccttctcca gcgcattggg ctgtacggca
    11881 agaggcactc ctatcccctc cagctttccg gaggccaaaa gcagcgcatc gccatcgtgc
    11941 gcgccctggt catgaatccg gatgtcatcc tgtttgacga gccgacatca gccttggatc
    12001 cggaaatggt gggagaagtg ctctccctga tgaaggagct ggcgcaggta ggactgacga
    12061 tggtagtggt tacccatgaa atagggttcg cgcgggaggt tgcttcccgc gtcctgttca
    12121 tggataaagg gaaaattgtg gaagaagggc ctccggaccg cgttatcgac cgccctgcca
    12181 atcccaggct acaggagttt ttttccaagg tactctgaca cgagcagttc tggaaaaaat
    12241 acaggggaag ggtggaagaa cctctctctt accgcctgcg cctataaaca aagcaatttg
    12301 gcataacctt ctctttcctt ccccggacaa tggaaagcct ctgttctcta caacatgatc
    12361 tccacgttat aagactcgtc aatcaacaga tagtcacagc catagaacct gcatttttca
    12421 agattgcccc tgttttcacg cagcaactct tcccgcgtaa cgggagaact ctctacgcgc
    12481 cgttcaatcg cattctcatg gttcaggata tcatgataat gcctttgaat ataattttct
    12541 gaaaaaataa ggcatacata ccgtatccgt tcccggcagg cctgcgtaaa atccttcttc
    12601 cagtcaaagg gaatatagca accttccacc actaaatttt ggccattttc aatcgcggtc
    12661 tttactattt ctttgataat gccccagagg aagggagtca gctcttcatc ggggctttca
    12721 ggggagaaag ggcatatccc gctgcgtatc aaccccatct ttaaatggtc aatggataaa
    12781 tagggatagg aatatttttc aagcacccgc tgcgccgcga gtgttttccc tgtatgcgtg
    12841 ctgccgccaa tcaaaagaat cataagactt ccctccctgg catttcataa gaaagacccc
    12901 caatctttcc catgcagcca atatccggca tcctttcgta ttcgttggtg atatccatac
    12961 ttaaccagct gccaacatac tcacaggatt ctcggcatgc agtgcattcc gcaaaataat
    13021 caccttgaac gtcaatcgta agcaatgcct gcatcagctc gttccgtctt cattctttca
    13081 gttccttccg gaacggacaa gaaaaaacac aagggcgccc gcccctctcc gttgcccgcc
    13141 gggatgaaag ctgttacggt tcccgtccgc agaaataaaa ataaccttcc tacagcaaag
    13201 cctttgccgt ttccggcaaa ggctttgagc acggcgtccc attacgggcg caaaacggga
    13261 accccctaat tgccgtaaga ggattccacg cgcctggcta cgtcgcggta gccgtactcg
    13321 gcgtcgtaaa tctgctggaa tgcagccagg gattccccct tcttgccctg cttttcataa
    13381 agcaagccga tcatgtaaag gatttccttc ttggtgtcat tcatttctat caattcctta
    13441 ttggcttcct ccagctggcg cagggccata tcatgcatgt tcttggcgtc atagcacttg
    13501 ccgagcaaca gcatggcgcg gatgcggata tgggggttgt tgcgggcgcg ctggagttcc
    13561 ggaatggctt ccgtgtaatt gccgcagtcg aagagggcct ggcccagctc aaaacgggtt
    13621 tgcgggtccg tggggttgtt ttccacacgc tggcggcaca aggccacctg ctgctccgca
    13681 gcttccttgc tgaactgggc aagctgttcc tgaagttcct tattatccgg ctccgcggca
    13741 gcgcggcggc ggatttcctc cacctgggcc ttgcggcatc gctcattcat ttccgaggcc
    13801 ttgttttcca gggaaatatc attgttgctg aggctgaacg cgtaattata gaaggaatag
    13861 gcattggccc aatcttccat ttgttcataa acgccggcaa tgtcgcgcac gacggccaga
    13921 tcctgctggt tctgggcgta acgggcggag agaagcccca ggcgctcttc cagttccgca
    13981 cgggtgagcc ctttcttgtc gctcttgtcc agggtgttcg tttccaacga gttcttcatc
    14041 acgtcccgga agcttttagc ctcctcccac ttctgctgct gcatcgtggc gcgcgccatg
    14101 cagtcctttt cgctctttac ggccacactg tccgtgggat ccagcttcac aaggtcatgg
    14161 tacacttccg cagcctgggc cggcatatcc cgggaaacgt aatgggaggc cagcatgtgg
    14221 agcattttct tgttgccggg atgcccctgg cgaacggtct ccagagcaaa agcggccaga
    14281 tccgggaaat ttacttccat ggcagccgtg tacagggcct cgtttaccgg aatgctgtaa
    14341 ggatcatttt ccaattcgtc ttccagagcg gaaagctggg tagccgcgtc cttcttggaa
    14401 gaagtgagct tgctggtgct gattttcatg ccgctgaaca gcccccctct cttggcctcc
    14461 ggattcaatt tgatttcaca ggcgcgcaac gccttgcggc cttccagaaa accggggagc
    14521 tgctttacca gggccttgag taggctgacg gcatagggat aattgttcat gtccaccgcc
    14581 tggcgggcgc gcacccaaaa ttcggcctgc tggggagtca actgttcttc tgtaatcgtt
    14641 tcaatcatgg aactatataa gcgtaaatgg tgaacaacgg cagcgcccca ccagggcggc
    14701 cccgcatgta atagtttaat gttatcagcc ggggaggcat acttcaacct tgttttttgt
    14761 tcccgttagg aaaaaatatg ccgcatgaga ctggatgccc ttttatcccg ctacggatat
    14821 tgttccagga gggaagcgcc gggttggatc aaacgccact ccattacatt caagggaaaa
    14881 ccatgctcct cccccacgga caaggtgcag gcggaaggca tcctgctgga cggagaacct
    14941 gtggaattcc cggacggcct gtacgccgcc ctttacaaac ctgaagggta cacctgcagc
    15001 catgacgacg gggaggggga tttgatttac gacctgctgc ccttccagtg gcggcacagg
    15061 aaccctgcgg tttcttccgt aggacggctg gacaaagaaa cttccggcct gcttctcctc
    15121 acggacgacg gaaaattcat ccaccgcatg acctcccccc gccatcacgt cgccaaagtt
    15181 tacgaagccg tcacggagga ggacattccg acagaagccg tggagttgtt cgcctccgga
    15241 accctcacgc tgaannnnnn nnnnctgaac ggggaaaccc gcccctgcgc ccccgccctt
    15301 ctggaaatcc gggaaccgcg acgcgccctg ctgacgctta cggaaggaaa ataccaccag
    15361 gttcgccgca tgctggctgc cgtaggcgct cccgtcttat ctctgtgccg tgtttccatc
    15421 ggctccctgc atctggacag cctgaacctg aaaccggggg aatgggcgcc tatccgtccg
    15481 gatgcgctgt aaacctccca tccgccatgt ggattcccat ttccgaagca ccaattgcct
    15541 gttttccgca ctttgaggtg gcggcggaat ccggggactg gattgtggtg aacaagggag
    15601 cccccctcat cgtccacccg tccaacggga aaaaggaacc ctgcctcctg gaaggcgtgg
    15661 aagccctgct aagctatgaa atagccaacg gagccgccct ctccctggtc aacagactgg
    15721 acagggaaac cagcggtctg acgctggttg ccaaacacaa aagggcggcc agagagctgg
    15781 gaagagccat gcagcggcgc ctgatacaca aggaatatct ggcaatcgtg caggaatggc
    15841 ctgaatggac ggaaacagca tgcaccgccc ccattttaag acagggggaa gtcgcggaaa
    15901 gccgcatatg ggtcaaacag gccgtgcacc cagccggaaa agcatgcact accgttttcc
    15961 ggaaagaacg gacatggaat acacgccggg gccccctggc gctggttcgg tgcattccgg
    16021 aaacgggacg catgcaccag atacgcgtgc atctggcgca cctgggacat cccatcctgg
    16081 gtgacaaaat ttacggtccg tccgaacatt gctatctgga atacatccag cacggatgga
    16141 gccgggaact ggaggagcgg ctggttctgc cgcgccacgc gctgcatgcc tgccggctgg
    16201 aatttccgtt tgatgaagaa accttcaccg cggaagcccc cctgccggaa gacatgcggc
    16261 tactgcttga aaccggggat tagtaaaaaa ctccgtcaga tgaccggaat gacttcatcc
    16321 cggtcaaaac gtactttcgc cagacgggcg tacttatccg cctcttcatt ccggtaaccc
    16381 agagccaggg cgcaaagcgt gtaataaggg gaattttcca gatgaagaat gcggtcatag
    16441 gcggccggat ccatcccttc cagcgcgcag gcgtccacgc ggagatccgc ggcgcagctc
    16501 atcatgaacc cgagggcgat gtacacctgc cgttccagcc aggctttcag cacttccgga
    16561 gacttggaac ccaccagttc aaaaatgcgg gcgcggtact ggtccaggga gtccatggca
    16621 acaccgcgca cttccacaat acgttccaga tagcgctgca cgtcttccgg cttgaaatcc
    16681 ctgcgggcac agaaaaccac cagatgggaa gcgtccgtaa cctggggctg tccccatgaa
    16741 acttccctaa gccgggaacg cacggcggga tcccggacat caaggaactt ccacatctga
    16801 agccccaggg aggaagggct caaatgcagg gattccagaa gggcttccca gtcctccgcc
    16861 ggaatgcggc ggtccggatt gaataccttg gtggcgtaac gccattccag cgtctcgatc
    16921 aattcctgag cgttcatatt catttatgat tattcgtgca ggaagccgga cggttttccg
    16981 ccgcagcttg ctccatgccc tggcattttc cccgtttccc tgccggaatg caagccaagg
    17041 ttccggcaca agcgcggcag cggctccttg cctcttcttc ctttcttcct gtccggcacg
    17101 ccggaaacag gccgctctcc atccttccaa ccgcatggct ttccgcctct tgccgaccag
    17161 ctacatatct ttccctttcc ggaacaacac cccgcaccgg gcagaaccca ttctcgaaac
    17221 gcgaatcctc tgaaaaagga aaaagcttcc gggaaaaacc tcgcaaaaaa ccgtgtcctt
    17281 tccggacatc aaaggaattt gaccggcata taatccccgg tctgctggat ggtaaagctg
    17341 atgcccagcc cgaattcgtt tttgttcccg ttcttgcgaa cgaaagcgcc cacgcccaaa
    17401 taccaggatc ccatattgtg gtacacattg tattcctgaa tgtccagctt gccgtccaac
    17461 agggaaaaac gccatttgcc gctgaaagcc caggcttccg aaaagcgctg caaaatacgc
    17521 agatccagct ggctgctgtc ctccagaagg gaatgctggt tcaaataacg gtgtcccacg
    17581 acaagctccg tggaacgcca tggcatgaaa cgcagggagt tgttgtattc acggcagccg
    17641 gaaatcttgt ccttacccaa aacgggagcc tgcatttccg accggtattc catccaggga
    17701 acgggattcc agcgcatgaa tgaaaaaagg ttggaaaaat cccgctggtt gacgggatca
    17761 tgcagatagg catccatgaa cacatcccag gaaaaccagc ggtgggaatt agcgtcccgg
    17821 ctggtcatca gcatgttgcg caatccatac cggaagacaa gccccgtgga cagggaatca
    17881 atctccgtgt acctgcctat gctcaaggat gggggattgg tcgtgggggt atcgccgtca
    17941 atctggggat aaagctcgct caggcggttg gttttgacat acgccagcgt aaaatggggc
    18001 tgaacaatat ggttcatgcc gttcagccca aaggaatcac tatatacgga agaataacgg
    18061 cgggagaatt tgaaatcggc atccgttccg gcgtaaaaaa ttccctggtt gagcggtttg
    18121 tagtcctcca cgccgtaata tcccgtatag cctccgccca ctttgggggt caggttcagg
    18181 aatcccatga ttttcttgga aacggaaacc tcatggaagg agtggaacct gctgtagctg
    18241 tccgtcatca gcattcgggc ccagtaatca taggaagaag ttccggggtc catcccgtcc
    18301 agcatgttcc ggatttccgt ccgcatgaac ggaggaacgt actgcttgag gaaagcgaag
    18361 ctggtgcggc tttcatacat gacgggggaa cggaatacgg gggacttgat gcgctcataa
    18421 ctgaactccg tccgctggtc cgctatgtaa aaattattgg ggacaaaacg ctgcaacagt
    18481 gaaaaatcat tcgtatcgtc cgtacgggaa agaagcaccg tgttgtccgg agaagaattg
    18541 cgctgataaa tttccggata aaaatcgcgg agcatgtact cgtcgctcaa catgttgatg
    18601 ttggccttca accgccagtc cgtgcggaga ttcaccctgt ggctccacat ctgctggagc
    18661 gcgaacctcc atcggtccgg gtccagatgc tcacggtact catcatcacc ggcgttgatt
    18721 ttcaccccct tgtcatgggt tttgtaaaag gacagccccg tcatgtccgg acaatccttt
    18781 tccagcagca cgtcgcggat gtccaatccg taggcgaaac cgcgccgggt gcggtaatct
    18841 gcatgcaggg ttcccaggta atccgcggaa ggcatgccgt tgtccgacca ttttttcccc
    18901 atcaggaacc cgtattcatt caggaggtaa ggcccccaga tggagcgcat gccggggatg
    18961 ggaagatagc caacttccgg attaagggaa tggctgaggt aggggaaata aaagaaggga
    19021 acgccaccgt aataaagggt caggttcttg aaagaaaacc tgtcttccgg atacactcgt
    19081 atcctgtcgg cggaaatcca ggtgagaggt tcgctgannn nnnnnnntgt cttccgccgt
    19141 gacggaggca ttgcgggctt ccaggtaagt gttgccggcg gcatccttcc gggattcaaa
    19201 ctttccgctg cgcagaatca atccctccat cttcgcacgg atggcatccg taagaagcac
    19261 ttcgttctcc cagtcaaact ccgcgctgtc ggcgcgtacc aggctgttgg tccttgtcct
    19321 gccgtcactc tggtaaatgg ccaggttccc tttcagaaac accttgccct ggtccatatc
    19381 cgcctgggca taatccgcaa atacctccac cccggtatcc gtcaccatgt ggacatgcgg
    19441 tccttcaaaa ataaccttcc tggattcctc caccctgatg gcgccgtcat tggtaatgga
    19501 aatattggag ggaatctgcg gcatgagagt gccggaaagc agattgcccg ccattttccc
    19561 tccggcattt tcctgcccgg tttcagagga agggacgggc ggctgctccg cagccgtttc
    19621 tgcaaacagg gaagccccgg caaccagcgc cgccgcaccc catagctttg caatcgtccc
    19681 aatcatggct agcccacaga acactatgag aaagggcaaa agacaagcct aatgtcacat
    19741 ttctccgcca gtgggggcag aaaatttacc gggtcagcat gccgttggct ataatgcccc
    19801 aatcggcggc attgccgtct ccggcgtcat ccacgcggat ttccagcttc ctgccgccct
    19861 ctaccgccgt cttcacggga atggagtccc cgtccttgag cacaggggat tccataagct
    19921 tcctgccgtc caaatacacg ctgaatttga cgctgccgcg ggagccgaag ggcagcccca
    19981 catcagcgcg gaattctttc cagcgcccat ccaggtcata ggcaaaaaca gccggggcat
    20041 gagccagaat gaaacgttcc ggccttccga ttgtccggaa gaaaggcacg ggccccaaat
    20101 ccgacgggaa caggacatcc cacaacggaa cggcccaccc cacctgggct acggaaggct
    20161 tgcagtcatt caggctgata ctggcggttg ccgcaggaat ctgcgccgga acaacttcca
    20221 gcgcgggctc ctggcgcccc agagcgcgct tccagacagg gagccattct ttcacttcag
    20281 aacgggaacc atgcctgcgt tccacttccg ccagcgcctt ccgggcttct tccgtgcggc
    20341 cccgtatcca cagattctga acatccccga aataaacgat ctgggccaga gaaggggcct
    20401 tggctccccg ggcatccatc cacacgggca gagtaatcat gctgcgcgtg ctgtcgcaat
    20461 tcaatacggc cacccgcatt tctataaaac cacccttata tcccggcctg cagatggtga
    20521 gatcaaactc tcccttttca tccagagaag cgcccacggc attggaatca taatccgaac
    20581 ctccgggcgg atccaaatgg gcaacaatgc catacgcggg gcgcgtcaga tgaaccctcc
    20641 ctttcagccg caatccgtct ttaacgggaa tggcctccag ccgttcaaaa gaccccggga
    20701 catacttgcc gagcatacgc ccgaaggagg cgtctgcggg aagttccgtc tccaccccgt
    20761 tgaacagggg aacactggcc agtttcaaag catccgtcgg cgccaaatag gcgcccttcc
    20821 cttcatggcg cagctcgtcg ccgtaggtgg aattgccatg gcccatcagg gaagctccgg
    20881 cgtcggggta attccatccg tcgcccgtgt ggggaaggcc gaaggaatgc cccaattcat
    20941 gggccgtacc gcctatgtaa atggtggcgt tctttcccct ggtcaccttg aaacggccgc
    21001 cctgcatcat ttccgtatcc agaaagctgg cgggatccag cccttcctgg tcacaggtcc
    21061 accccgtgcc ctggtggctg aagccgccgc cgtaataagg acccacgccg tccggaagct
    21121 ggcaaacgac cagcacatgt tccttctcaa tatcaatccc cttggaagcc agcaccttcc
    21181 tggcggcttc ccgggagacg gggccggagc tctgcacgct catctcgctc atgggtttat
    21241 ccacataggc atcatgaata accagcttgc cccgttcatc caaatccagc tggaatgtga
    21301 gcggagggaa accgttggcc tgcatctggt ccgcataata tgcctgaata ttcttcagaa
    21361 tatgatcata ccgttcccgg tatccttcaa gggcaggcct gtccttgaac gttacgtaaa
    21421 ccacatgcaa ttttctggaa gacttcggtt tgccccgatg ataggcgctc agcgttttcc
    21481 aggcggcacg ggcattggcg gcatccctgg gattaaccgt ccagctgacg ggcagacggg
    21541 taaactccgc tccctccctg tccgaggagg caggaacaga tgccctggaa gctgactcct
    21601 catccatccc catggcagga acactccata tcccggaccc caggatcagc aacaaggcaa
    21661 aaaaacgttt cgacataaca ttcatgaact aatatcagga tatacgggta aaccgaaggt
    21721 gttttatcac catatcattc accagacgat tcatattttc ggaaaaggaa tcatacacca
    21781 cttccacacg gcagggaccg ggcgtcatca ccgtttccac tgccgttgaa taaataaaat
    21841 actcccagtt tccggaacgg gacgtatggg ggaaagccag cgtgcctgcg cgcctgccgt
    21901 tcaggtacag actgcgcagg gcgcaggtat tcccgtctga aacatcataa gtgccgttag
    21961 cgaaaaaggc gtccacccgg tacactccgg gcttatctat attgacattg taacaaagag
    22021 cgtccgggga ttccgtgacg cgggaaaacc agacttcatc cccgctcaag ccgcaggggc
    22081 gcgtctccat gcgggaatcc gcagaaggat aatcattggg ttcattaaga taagactccc
    22141 tcccatccag agcgactgcc atcacctgga aagacacgtc cccccggccg ggtgcgggga
    22201 tataatggca gtactccgtt tgagatacag ggatgccatt ccggtaaaca cggtaataat
    22261 cggctccttc cacagactcc catgcaatac catggcgcgc cgccttccac gcaggagact
    22321 ccaggctgct gcccatgcgg gttaaattca ccgccccttc atcctcttct ccgccgtcca
    22381 gctccaattt cacaaaaacg cgccccttca tgcccggcaa aaggacggga ggggcctcct
    22441 gcccgttgac caggcaccgc gctatccggt gcccgcagcc ctgcagaaac acatctaccg
    22501 tcatgccgcg gtattccagc ccggacaact catgaacgcc ttcaaaagac tttggaacgc
    22561 aaggacggaa ttccagagaa tccggagaca gacggatgcc gaacaatccc ttgtaaaaac
    22621 agcccagcat gccggcaata ctccagagct ggctatccga actgagcagc agcccctcat
    22681 ccagccccgt ttccagaagg acattttcct tgttcgtgcc gcacaacagg gcggcacgga
    22741 ccatgcaggc gaccgccagc gccagggcag attcattctc caccgccgcc gcggcctgcg
    22801 cataatagcc ctccaggaaa ggccacatgg cgcggttatg gtaggcgggc acgctatcag
    22861 gcatctgggg atggatgcag ggaatgccat aaacgccatg aggaagggag gcaaccatcc
    22921 cggcggcatg ggaaccgcca gcctgcccca gaaggacgca aagcagattg cccagggaat
    22981 ccaccttctc cgacagtacg ggatacccgc gtccgtacag gtactgcccg tacaggacat
    23041 gttccggaat cctgaaaaaa cgctcaataa ccgcggccag ggaaacgctc ttctcctccc
    23101 actcccgggc cttctcctcc agccccagct cccggaacat ccgcgccaga atcttgcggg
    23161 cctccgcatg caggagcatg gtggacaggc tgcaagaatc cccaatatcg gcagaagtca
    23221 tccacgcagg gtagctctga tccctccagt ccagcacgga cgattccccc ttcatcaagc
    23281 cgcccgtggc cgcaagcacc tgttcatccc tcatgcatgt cttttccatc actcggcagg
    23341 aaagggacag ccagtcggca tcccccgtca ggcagtaaac ttcccacgca gccatccccc
    23401 acaccacacg gtcggaagaa atgggccagg aaccgcccgt gccggtatcc tgctccacct
    23461 ccccgttccg gacccgtgca tttagactgt tcatacaggc acggacattc cacaacccca
    23521 atcccagatg ggtggaatag gaaatatccc gggtccacac agtaggccag ttggctcccg
    23581 tccggaacgt tccttcttca ttaagcaggg ctccagcctc atgcaacgcc aggccgtaag
    23641 cctgattaag cacttcaaaa tttccccggt aatgcggggc gttcccggct tccgaaacaa
    23701 tgctccactc ggaatagcgg ccgtttttca tggttttcac ctgaccgtcc ggctgtacct
    23761 ccgcccaatg cgcggcatcc tggcgcaagg aatttccggt cagcgtaaaa gcctctcctc
    23821 tgtataatat gcggtcctga tccataaact agcggatgct gcatccatcg ttttttttaa
    23881 cacggaagaa aacttctgtc ccgcagaaaa tgaggtttat ggaatcttca ggacataaaa
    23941 aaccgggctt cccgaaaacg ggaaacccgg cgcaagagca aatcaacatt aatgcttcaa
    24001 caaagaggaa acagcgtcac gttcttcctt cagttcatta acggtagcgt caattttttc
    24061 acgggagaac gcatcaaccg gcagcccctg cacgatttcc cagccgccat ccttggtggt
    24121 gcggaccggg aaggagcaga taagaccttt ttcaatgccg taggaaccgt cggagcaaac
    24181 ggccacggaa taccagtcgc cttccggagt ctgggtagcc aggctgcgga cggtatccac
    24241 ggctgcggaa gcggcggaag cggcggaaga agcgccgcgt gccttgataa tggcggcacc
    24301 gcgctgctgc acggtggtaa tgaaatcgcc cttaagccat tccgtatcct tgatgacttc
    24361 cgtcacaggc tttccgccaa tcctggcgtt ggtgaaatca gggtactggg tggaggaatg
    24421 attgccccag atggccatgt tggtcacttc cgtcacatgg acgccggcct tttccgcaag
    24481 ctggctcttg gcacggtttt catccaggcg agtcatggca aaaaagcggt cggagggaac
    24541 gccgctcgca ttgtgcatgg caataagggc attcgtattg caggggttgc cgaccaccag
    24601 cacacgcaca tccttggcgg cgctgcgggc aatggcctgc ccctggccga tgaacacctt
    24661 gccgttgata tccagtaggt ccttgcgttc cataccggcc ttgcggggaa cggagcctac
    24721 cagcaggcac cagttggcgc cggcgaacgc ttcatcagga tcgctggtcg ggacaatttc
    24781 attaaccagc gggaacgcgg cgtcacgcaa ctccatcacc accccttcca gagcattcat
    24841 ggccggggga atttccagca ggcgcaggtt gatcggctga tccggcccca gcatgctgcc
    24901 ggaggcaata cggaacagaa gggaataggc aatttggccg gcggctcctg taacggtaac
    24961 ggtgataggt gttttcataa cgttcaaatt aaaataaaca gatattttcc tgtctggtca
    25021 cggttatacc acaaggaaag gaggcgctca atcaggaact ttttattttg acagaaaaaa
    25081 ttccctatcc cataatgctc aggggttgac tgctcttact tttccataca gttaagtttt
    25141 taccaatatt tacgctaaca ctatgaattt aacaattatc ggcagtggtt acgtggggct
    25201 gaccacaggc acctgttttg ccgaaatggg ccatcacgtc atttgcgtgg ataataatac
    25261 ggaaaaaata cgtactcttc aatccggggc catccccatt tacgagccca agctggaaga
    25321 gctcgtgaaa aaaaacgtag ccgtggggcg gctggaattt tccccttcca tcgccgcttc
    25381 cattgcggaa agcgaagtca tcttcatcgc cgtgcccacg ccgcccaata cggatggttc
    25441 cgtggacctg acctacattg aaaaagtggc cagggaaatc gcccaggcgc tccaaccaga
    25501 aatgggatac aaggtcattg tggacaaatc caccgtaccc gtcaaaaccg gggaaaaagt
    25561 cagccagacc atcaagca
//
LOCUS       DAWEMX010000020        25003 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_803_length_25003_cov_11.491222, whole genome shotgun sequence.
ACCESSION   DAWEMX010000020 DAWEMX010000000
VERSION     DAWEMX010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 25003)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25003)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25003
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_25003_cov_11.491222"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9981..9990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5692 a         5739 c         7376 g         6186 t
ORIGIN      
        1 tcccacggca tcaagttatc ccttctcccc ggtatcatga gggcggtaaa agccggaaag
       61 gtgcaggtgg ccggggtgca taattttttt cccgctccga ttgacgaagt gggggattct
      121 ccagacagca ggccttttac ggcggatttg ctgcaggtgc gttccaaggc tattgaattg
      181 acgaaaaagt ctattgaaca gggcgcagca ctgggggccg gatatatcgt gttgcatatg
      241 ggaacggtgg aacccttggc gaaacgcacg gatacggctc atttgcaaaa cctggcgcgt
      301 cagggaatgg tgggaacgga atcgtttgcc ggcaccaagg gagaatttgt acgccgccgc
      361 aaccgcctgg ctcccgttta tctggaacgg gcgcgtgaag ctctccggca gctccttccg
      421 caagccggac aatttagcgt caagctgggc attgaagggc gcagccatta tgagcaggtg
      481 ccgagtgagg atgagatgaa tttgttgatg aaggagtttg gagacaatcc ctttatcggt
      541 tactggcatg atttcgggca tattcaaaga aagcataatt tgcttatatt aaatcatgag
      601 cagtttcttc gccgaatgtc ctcccatttg attggaggac atgtgaacga cgtcaaatgg
      661 cccgcgcggg atcatcaggt tcccctgctg ggaggagtag ttccctttga acgccttctt
      721 ccttttttcc cgcatggggt tccgctcgta tgggagctct ccagccgtgt ttcctcagaa
      781 gatatccgtg cggcgcgcaa attgtggatg gaaaaatttc cgcagacgct ggcctgacta
      841 tcttgtgatt gtaaatccgg ttccgtgggt atagagtcgg cacgatgata gataagacga
      901 catcccgcgt cattatgggg cttttgggaa cagcctgtgt cttgattacg gcatttgcct
      961 ggtacaagac gaaatatgcc gccgttccgg aggccgggat gcaccataat atttatgacg
     1021 aagacattcc gcggtctgct ccggttcctg tagattaccg gatggttttg ttgacgcccc
     1081 aggagctggc aaaggcgcct ctggccgatg tgtttacatc gcccctcgga gatgagaatg
     1141 gagcgtttac ctatgttgcc cagggtgtgg gggatatgaa tgccgcccgt aagggaaggc
     1201 atgcaggaca ggatttaaat ggaataggag gagaaaacac ggatgaggga ctgcccgtgc
     1261 gggccgccgg ccgcggattg ctgatttatg caggagaacc ttctccagat tggggcaatg
     1321 ttgtcgtgct gctgcaccgc ttgccggacg ggcgttttgt gcagagtctg tatgcccatt
     1381 tgaaaaccgt cagcgatatt ccgctgggga ctctggtggg acgcggagag caaatcggct
     1441 cagtcggaac tgcgcacggc aattatttgg cgcatttgca ttttgagatg attgaatcca
     1501 tcgctcatga ggcgggaatg ccgggctatg ggaagacaac gtttaacaga atcaatccgg
     1561 atgaggtgct aaagcaatat gctcccgacc cggaaatgat gatgcctgat cctattattg
     1621 ccttgaaaca ggttcagatg gcggcggggt gggaaaaact gctggagaat ctgtataagg
     1681 ataacagcat ggaagctttg gataaaatcc tcccagacag tcagccgccc acggaagaaa
     1741 aggaaaaacg ctgattacgg ttttttcgat caggaacggg agttttgatt tttggagacc
     1801 agttcctcct gttttctggt gatttgcagg acacagacca gccacaggat gctggctgcc
     1861 gtcatcagta cgccggtgag cagaaaggcc gtcacgtaat gttccgcccc gaaagggaaa
     1921 agggtgcaca gagttagagc cgtagtcacc agaagatata agcgcaggga acgggagggt
     1981 gtttgcaaca tccattctgc cgggtttttg gatgccttat atatttcctg aagaaccagg
     2041 gcattgagca ccatgccgac taccggcata cagcttagaa gaacgctaag gaatggagtg
     2101 aaccgaattc cctgaacttg aaattcctga atattggcag atgcccggta cagccagatc
     2161 agctgaataa gcagggatgg aatgaacagg atgctgagga agaagaccaa tggtactgca
     2221 aaagatggca tgtgaaccat attctgctgg atgagtgtct gaatttcttc ataattgacg
     2281 acattgttgg aaaaaacaat ccagctgctc cagagcatga agggcagcaa tacgaaacat
     2341 gccaaaatgg ataatttggc tagcccgttt agaggataac gcaagcggta ctgctgctct
     2401 tctggctgat ctttgccctc ctgatcctgt gtttcctccc atacaggtga catcggagga
     2461 agaacttggg ctacgggctg ccatccttcc atgccctgtt tccaggtaag cgtttttgag
     2521 tcgatcacgc cctgtcccag aaattcacgg acttcgtatt cggaataggc tctcggctgg
     2581 gaatcgccgg gaagtttcag gaagtattca ctcattacct gtattttaga gaaagaaaca
     2641 tgcgcggggc aatgggaatt tgacggatga ctgataaagt atcccagcag gggctttttt
     2701 cagatagaag aatcagctgc tagtgggttt ctgactggct tttgcagggg aagtgatttt
     2761 accggaaaat aaaataaccg tccggaagag gttgttagaa aatattcgaa ccagcggtct
     2821 cttcgtcccg aatgaagaaa atttgaaatg taactattga taatcaatga ttgtgtttct
     2881 tgtgcccttt tcaaatcctt tgttttccac ttgattgtcg tgccgtttta accttttcct
     2941 tcacgttccg aatgagggtt ggattacacg ttaaacgtgt tgataaggag cgagaaatct
     3001 ttcaaggttt ccatgaactg aatattcagt agtaaacgat acgaaacata atattatggc
     3061 tatatctaca aaaccgttcc acccgttgga tgtggaaaac aaccgccgct acaaggtaac
     3121 gaagaaagag tcccccaaga ttggatggca caagacggaa gaaaccgggg acatgactgg
     3181 gaaggatata tccgcgttcc ggaagatgga acctataatt tcactattca gattgacgac
     3241 aacggctatc tggaaatcaa tggtgaaaag gtagcggaat tgactggctc acattcgtcc
     3301 acatccgttt ctggaagcaa ggaacttaaa aagggcttcc actatgcgaa actgcatcat
     3361 gagaaccttg ccgtcccgga agaaattgct ccttacccca atgcggagga gtttgttccc
     3421 aagatgggag attccgaact ggaattttgg gaaatcgatg ctccaaaaaa tctttggaat
     3481 attgccaatg cacaacgtct actgggatgc tataatgtag tggattatcc caccatgact
     3541 acagatgatg tgtggaacta tattggagga tggctgaacg atagccacgt ttcgggagat
     3601 gccaattaca ttaacagttg cgccttgcgt ctgagcattg ctcttagtag ttatggtatt
     3661 gaccttaatg gagctccggg agccaataat attggagcaa agggtaattc ccaggcgttg
     3721 gggggtaaaa agcacgttat catcagcgca gctcaaatgg cggtttatct ccgtatcctg
     3781 ctgggggctc ctgattatcc ggatgatgga gaaaatgggt acaacactcc ccaggctgga
     3841 gacattatcg tatttggcga tagtctgcat gtgggaatgt gttcgggaaa taacattggc
     3901 gtgggcagtt ttatttccgg ccccgtgtgg ctgctccaaa gatcgacact ggatgactaa
     3961 taactgatat taaataatat attcataggg tggacatttt ttgtgtttgc tctattttta
     4021 ttgaatataa accaaaaaat cagtatctaa tatatacata tgaagtatgt ttcttttctt
     4081 tgcgttctgt tttcaggatt actttcaacc gtagcccttg gagagccgga ccgttcttcc
     4141 catgttgcta cgcaggccga agtagaacag gatagttttc tcaggggtac agcccgaaat
     4201 ttagaaaaaa gcgatgtggt cctgtctgtt atcatttaca aaatagtctc cactgaccag
     4261 ggaacgacgc actatgcccg cgtcgttcag tcgctgcggg gagatattcc tgtagaagct
     4321 ctggtcaagt ggaacgggca cgccaataag cttcccaagg gaagcgctcc taaaacggaa
     4381 ttgtattcca gttgtaatct gagttatgtc cttgcgtctt caaaggaggt aaaaaaactg
     4441 ctgaacacac cggaagattt tactccggct gacaacattt atggactgga ttcctacagc
     4501 ctgggcgacc acgtcagata ttttccgatg acggagagcg acccgggccg tgccatgaaa
     4561 aagctcctgc atatcgagcc tgcaaaaatc accgccgaat cggaagcagc tcgtttcaaa
     4621 ccggaatccg gcaagtaacc cggttttatc tccctgcttc gacttcgttt gactcatccc
     4681 cgttcatgaa atatgaacgg ggcttttttg tggaggaggt tcattctcct ttgtttaggg
     4741 ggatgttata gagtacttcc gcagcggctg tgatgctgcc tggcacaggg gtgagaattg
     4801 ggcatagata atttttcctc tgcatcggta aatacccccg acatcctcca ggcttaattg
     4861 agaagatttg cccgtttgat tttgttcctg tgaggagggt aaattcaatc agcatgatgc
     4921 tgtttatttg ccttggcgca aaatagggga ggtgcagagt tattaatgag taaaattgtt
     4981 ttatatcagt aaataagtaa tcaaaaagcc gcccggaggc ggcttggaaa ttggtcgagc
     5041 tgacaggatt cgaacctgcg acctcttcgt cccgaacgaa gcgcgctacc agcctgcgct
     5101 acagctcgtt gtttgtggtg cggcgaagtc taaggttgaa agatgggttt gtccagtttt
     5161 ttttattcgc cacggcactt agttgtgata cggtgccggg gttatgatgg atatgctggt
     5221 gcggctgtat gatttgcccg atattgaaga agacctgagg aggctggaga gggaaggttt
     5281 tttgattcgc cgtccgggtg cttacgaacg tcatttggta gctgactggg ttggccggaa
     5341 tttttctccc aagtgggtaa gtgaggcgaa agtcgcgttt ggccgccagc ccatttcctg
     5401 ttatattgcc acacgggaga agaagattct tggttttgcc tgttacgatg taacggctcg
     5461 cggttttttg ggccccatgg gggtggcgga agaagtgcgc agaagcggat tgggcaccgt
     5521 gctattgatt tccgccctga aaggtttgcg ggagctgggg tatgcatacg gtgtcatcgg
     5581 cggggttggt ccggctgatt tttatgcccg ggctgtgggc gcgatgccta ttgaagggag
     5641 ctctcccggc atctacatgg atattctgcc ggaaccataa ttccgcctgt tgtggaattc
     5701 atttccattt ctgatggttt cttttccgga ctgtccggag aatgtagccg gaagtagtta
     5761 ttttttatat cctaccggat gacagagcat gggtgtttgg agagaggtta gtttcagggc
     5821 ctttgccagg gcatccttgt tcatgctggc gcgcggatag gtagccagtc ccgtcccgga
     5881 gcagaacagg gagatgttct gggagacaat gccggcgtcc atggcagccc agggttccgc
     5941 cgtatccgtt acaagaatga gacatatggg ggcatccgcc gggcgcacgt ccccatcgtt
     6001 tacgggaatc atggcgtggg ccttgtggtc atagaggtag gaggcgtttt tagtgcatat
     6061 ataaatttta acgtcctggc ggttcatggc ggacggggct gtacgtttgc cggattcctg
     6121 acggttgatg ccattggccg cccagagcag gtctgataaa tctttttggg atagcgcttt
     6181 ttcagaaaag ctccgaatgg attgacggtc ggacagggct ttcatgatcg gagccccccg
     6241 ttccaggtcc ggctggtcca gtttgacggt atccgcggcg tgaaggaagg ctgttccgga
     6301 cagccccgct gcgagcaggt aaaggaggtt ttttttcatg gttttgcggc aggagtggtt
     6361 gatagatgca ggtattcctg cattttccca ttagccggaa tttaacatgg gacggaagtg
     6421 ctacaaggtt tttgtgcgct cgttttctgc cgccgtccag aggggctcca caggttccgt
     6481 aaaaaatcgg gaggagtttt tcaagaaaag ccttgagttc ttgtgtgaaa ctgatattga
     6541 aagcaaccga tttttcgccc ggcgggggaa ggcgccccga ttgcccgccg gtcaataagg
     6601 ggccatgaga aagatcgtta atactaactc atcaaccatt taaatgagca caactgaact
     6661 ggcggaactt attgacagca agttccgcga attgcgtgaa ggttccattg ttaccggaac
     6721 catccaagaa atccgtcccc aagtcgtttt ggtggacatc ggctacaagt ccgaaggcgc
     6781 tatttccatt tccgagtttg aagacgagga aatcgaagtg ggggaccaaa ttgaagtcct
     6841 tttggaacgc ctcgaaaacg acgaaggcat cgtcgtcctt tccaaggaaa aggccgccca
     6901 taagcagaac tgggataaga tcgtgggcgt gtaccgcgat ggcggccttg ttaagggtaa
     6961 agtgaagagc gtcgtcaagg gcggtcttat ggtcaatgtt ggcgtggaag ctttcctgcc
     7021 cggttcccag gtggatatta ttcctcctcg cgacctgaac gagtatgttg gaaaggttta
     7081 cgaattcaag atcgtcaagg taaatgacga ccgtaaaaat atcgtccttt cccgccgtga
     7141 ggtgattgaa gccgaacgcg ccgaccagcg ccagcgcttc cttgaaaccg tcaaggaagg
     7201 cgacaaggtg gaaggtatcg tgaagaatat cacggacttc ggcgcttttg tcgacctccg
     7261 cggcatggac ggcctgctcc atatcacgga tatgagctgg ggccgcgtga accatccgag
     7321 cgaaatgctc catatcggtc agtccctgga agtcgtgatt ctggaagtgg atcgcgaaaa
     7381 ggaacgcgtt tccctgggcc tgaagcagat gacagacaac ccctgggcgg atatcgaacg
     7441 caaatacccg atcaattccc atgtcaaggg ccgcgtgacc aagctcctgc cttacggcgc
     7501 ctttgtggaa ttggaaaagg gcgtggaagg cctggtgcac gtttccgaat tgtcctgggt
     7561 caagagaatc acccgtccga gcgatgtatt gaagctggac caggaaatcg aagccgtggt
     7621 tctttccatt tctgtgaagg aacagaagat ttccctcggt gtccgccagt tggaagacaa
     7681 tccctgggcg gatatcgaat cccgtttccc gattggtacc gtcatcaagg gccaggttcg
     7741 caaccttact ccctacggcg cttttgtggg actggaagaa ggcatcgacg gcatgatcca
     7801 cgtgtccgat atgagctgga cccgcaagat caatcatccc tccgaagttc tcaagaaggg
     7861 cgacgaagtg gaagccatcg ttttggaaat caagaaggag gatcagcgcg tctcccttgg
     7921 tatcaagcag cttgagtccg atccgtggga atccatcaat gaccgcttca aggtgggcga
     7981 tatggtgact ggccaggtgg ccaagattgc cagcttcggc gcctttgtga atttggacgg
     8041 cgatattgac ggcctgattc atatctccca gttgagcgaa gaccatgtgg aacgcgtgaa
     8101 ggatgtgatc aaggtgggtg atgaaatcac tgcccgcgtg atcaaagtgg acagcatcga
     8161 acgccgtatc ggcctttcca tcaaggccgt caattacgac accgaacagc tccgccgcga
     8221 aaccgcttcc tttgaagccc tccgcccgag cagcgatatg gtgggtctgg aacacgcctt
     8281 caatctggct acccgtgaaa acgaagagtg gagcccttct gaagagaagt aagcccgttt
     8341 tcgtattacg ctgattaatt taaccgccgc gaatggtcat attcgcggcg gtttttttct
     8401 cattccaaag aagtctggaa aaaatcttga gttagtttcc actaaagtat tagctttcat
     8461 tttataccat gttggagctg accaagagca atgaggatta tctggaagct atcgggttgc
     8521 tgtctgaaaa gaacgggacg gcccaggtga gggatattgc agagatgctt aaggtaaaga
     8581 tgccttccgt tacatctgcc gtcaagcaac tggcggatat gggactggtg gagtatacgc
     8641 agtatgctcc cgtcaagctg actccccaag gccgcagaat tgccgggaaa attattgtca
     8701 gccacggtat tctgtttgat ttcctgcggg aggaattggc cctcccggaa gaacgcgcca
     8761 atgaagtggc ttgccagata gaacacatca tgacttttga ggagattgaa aaactcaggt
     8821 cctgccggat ccgccctttt gagggtggag aggaaaattc cgccaagtag catggctccg
     8881 ggctctctta tgggaagccg gggatatgga tggccgagcc acattccgga aagaggggga
     8941 agggcagaag acggtgatgt aaggtaaggg aatgaaataa ggtttttgtc tgctgttttt
     9001 cccatggggc attgaaatgg ggaagagggc ttggtttcgg aaatattcat ttcctgttag
     9061 atgttcttcc tgcggaagag gagcggtttt gttcagcgtg cggagtttta ttattctcgg
     9121 aaatggcccg gtgcgagtcg gagtaaaaga ggatgactgg gagaaacggc attttgccct
     9181 tttccgtcca ggtaattcgt gataggttcc cgcgcatgga tccccatatc gctgcagatc
     9241 tctcggcgct cgacacggct gtcctgcata gccgtctttc cgagctcggg agctatcttt
     9301 gacctggacg gcattcaaca acgagtagcc gaattggatg aacgcatgag cgccccggat
     9361 ttctgggatg accagaatgc cgcacgggcg ctgatggcgg aggtgaatcc cctgaaacac
     9421 aggatggagg cgttttccgc gctgaagtcc cgtctggagg atattgacgc tgctattgag
     9481 ctggctcagg aggcggatga cgatgacctg ggccgggagg ctgtggaaga gtttgccaga
     9541 tggcagaagt ctctggcgga ttttgaattg ctgaccctgc tgaacggacc gcaggaccag
     9601 gcttcctgtt atgtaaccat ccacgccggg gccggcggca cggaagcctg tgactgggca
     9661 tccatgttat tgcgcatgta tatccgctgg tgtgagcgcc ggggattttc cgtgacgtat
     9721 ttggagagta cggacgggga tgacgccggc attcgttccg tgacattgaa ggtggatgga
     9781 gagtatgcat acggatattt gaagaatgaa cgcggcatcc accggctggt gcgcatttct
     9841 ccctttgatt ccgccgggaa gagacatact tctttcgctt ccctggacgc gacgccggaa
     9901 gtttctgatt ccatcaatat tgagatcctg gacaaggatt tgaaggtgga tacctaccgt
     9961 tccggcggca agggcggcca nnnnnnnnnn cctgccagaa tgagcgcagc cagcttcgta
    10021 acaaggaaga ggctatgaat atgctgcgtg ccaagctata ccagattgag gaagacaaaa
    10081 agcaggcgga agccgaccgc cagtacagtg agaagggaga catcgggtgg ggcaaccaga
    10141 tccgttccta tgttttccag ccttatcaaa tggtgaaaga tttgcgcaca ggcgtagagt
    10201 ccggcaatat tcaggacgtg atggacggca acctggatcc gttcattgag gccatgctgc
    10261 gcggccataa gcgcgaacgc tgatgttttt ttcttatggc cgccaaactg acgattgacg
    10321 ttttgtccct gttcccggat atggtggaag cccctcttgg cggcagcatt ctgggaaagg
    10381 cccgtgacag gggattgttg gagatacgct gccataatat ccgtgactgg acgacggaca
    10441 agcaccgcaa aacggacgat tacctttgcg gaggggggca gggcatgctg ctgaagccgg
    10501 aacctatctt tgccgccgta gaggaacttc gccggcggga aacccgcgtt gtgctgatga
    10561 ctcctcaggg ccgtacgttc aaccagtcat tggccgcgga attggctgcc tccggaggcc
    10621 atctcattat cctctgcggt cattatgagg gggtggacca tcgtgtagta gaagagctgg
    10681 tggatatgga attgtccatc ggggactata ttctcacgaa cggggccatt gcctctgtgg
    10741 tggtaattga cgccgtagcg cgcctgattc ccggcgtgct gggggatgag cgttcttccg
    10801 tggaggaatc tttttccaac ggtttgctgg aggctcccgc ctataccaag ccgaacgtgt
    10861 tccggggcct ggctgttccg gagattttgc tgggcggcaa ccaccccgcc attgaaaaat
    10921 ggaagcacgg gaagtctctg gagcgtacgc gcctgaaccg tccggatttg tggaggaagt
    10981 ggctggaatc gcatccgggg gatgccacag agtgatatat ttggctattg cgccaaattt
    11041 ctcttgccaa gttcggaacc ttccgttata agatcccgcc tgccaccgcg agggtggtgt
    11101 aaagtatttt tctgaacctt ttacaaacac aagatcatgt ccaagcattc cagcctcaaa
    11161 gcaaccggta ccgtaggcgg caagcgttcc gtcctgaagc gttttgaacg tgtcaagctt
    11221 ctgaaggaac gcggcgaatg gaagaagggc caaagccctc tcggcctgcc gaagaccaag
    11281 catgaagctt aagcttgtct ttatcctctc cggagagttt cccggcggag tttgcacggc
    11341 aagtcatttt cctaatacag gaagcctctc ggatgtctat gaaccgagag gcttttttct
    11401 tccggcaaaa gaaagcctcc tctttttgag gatgtgaatt gccgtaatat aaacaataca
    11461 aaatcatccg ctgagatagc ggacattata ccaatatgag tgaagaacaa catggctccg
    11521 aagaaaacgg aggtatccgc attaataaat tcctggcttc ctgcggcatt gactcccgcc
    11581 gggtggccga caggctgatt ggggaaggac gtgtggaagt gaacgggaag gttatcgata
    11641 cgcctgggat gagggtgacg gagaaggatt tcgtgaaggt ggacggccgc catatgaccc
    11701 ctatggaaga ggttgtggtg ctgttgaaca agccccgcgg gtatgtgtgc agccgcgaag
    11761 cgcagggagc cataggtact gtttacgacc tgctgccgcc gcgcctgcgc catctgaatt
    11821 atgtgggacg gctggatgcc gattccgaag gtcttctgat tatgacgaat aagggggaat
    11881 tgacccaggt tctttcccat cctaccggcg gcatagaaaa ggagtactgg gtgacggtag
    11941 atcagaattt tgataattcc gtactgatgc agtttttgcg cggagtacgc attccggagg
    12001 gaaatgccaa ggccaaatat gtttgccgcg cttccgcgcg ccgtgcctgc attgttttgg
    12061 aacaggggtt gaagcgtcag gtgcgtcaaa tgttccagtg cctgggcctt cgtgtccgca
    12121 agttggtgcg cgtgcgcatc ggctccctgt ggggagggga cttggaaccc gggggctgga
    12181 aattcctgga tgatgcggac gtggctctgt cactgaaaaa tcctccccgc cagcgtaaat
    12241 acctgggtgc ttcccagctt gtcgccgggg ggggcgccaa gggtgagcag ggcggaagag
    12301 gcgttgattc cgatgaggat tatgtgttta atccggaaga ttttgaagct ggggattctt
    12361 atgaacctac tccggaaatg aagactcgtt ttgtggagac ggaggatgaa cgtgaagtca
    12421 aggaagatgg atgtttccgg ggagattccg gcttccgttc ccgtgaccgc cgcggagatt
    12481 ttcatcgtcg tggagatggc ttccgcggcc gccgcgggga tggggccctg cgccgcgagg
    12541 ccggtttcag agaagagcga cgtaaaggag ggttccaacg ccgtgaaggc ggctttggaa
    12601 gagaccgccg cgaaggaggt ttccagcatc aggagggagg tttcagggaa aaccgtcgtg
    12661 aaggaggatt ccaacgccgt gaagacggtt ttggaagaga ccgccgcgaa ggaggttttc
    12721 agcatcagga gagaggtttc agggaaaacc gtcgtgaagg agggttccaa cgccgtgaag
    12781 gcggttttgg gagagaccgc cgcgaaggag gtttccagca tcaggagaga ggtttcaggg
    12841 aaaaccgtcg tgaaggaggg ttccaacgcc gtgaaggcgg ttttgagaga gaccgccgcg
    12901 gaggaggttt tcagaggcgt gaaggcgggt ttcgtaagcc gggctttgga ggcaggtcct
    12961 ccagcagttt ttcccgcgga aacaggggcc attaatgaat gacttgttcc ggtatgaaga
    13021 aacattgcag atggtggctt tttgccggat tgtggggact gtccctcgtt cagcatccac
    13081 ttcttgcttc tcagaatatt tcctctccgg agcctgccgg ggagggaaat tttgaggctt
    13141 acttgtccgg tctccgcgaa agagctctga atggggatcc agctgccgcc cgggagctgg
    13201 ctgtgcatta tgacgtggag ggtaatgctg cggaaacgtc caaatggatg tccgagtatg
    13261 tttccttggc agagaaaagg gccaataacg gagatgtgga ttccatgctg gatttgggca
    13321 agctgtttta tacaggcagc cgcctgtacc caaagaacct ggaaagggcc cggtactggt
    13381 ttacccgggc tgctgacagc ggtaatgccg cggcacagta ccaggtggct gtaatggctt
    13441 cccagggagc aggaggaccg aaggatgaag caacggcagc cctttattat aaaaaatctc
    13501 tccagacgtg gaagaaagag gcggatgacg gtgattccaa ggcggcgtta tgggctgccc
    13561 ttgtttatga acgaaagctg gttccggaca gttctccgga aaagtcagtc tcatatcttc
    13621 ttcaggcagc ggaaagcggc aacctgacag cacaaggcct tctggcattt aagtaccggg
    13681 atgggctggg agtgccgcag gatgcggcca aggccgtaga atggtttgag aaagcggcct
    13741 cccgtaaaga tttgggagcc gtgatggaac tgggcataat gttccgggac ggcaagtatt
    13801 tgccccctga ccgggaaaag gccttccatt ggtttgaaaa aggggcggaa tggaaggatc
    13861 cgtacagcat ggctgccctg gcggatatgc tgctggaggg aactccttcc gcagaacagg
    13921 cggcccgggc cctggctctg tatcgtgagg ctgccgccgc cggttatttc cctgcggcac
    13981 taaaggccgc ggagctgctc cagaacggga agggcgggga actggatgcg gatgaggcct
    14041 acaggctgct gcggcgtgtg gcggatgcta caggggatcc caaggccatg tacatgctgg
    14101 cccaggtata ttatacacgg ggtgatgagg ctcagggaga ttccctgatg aaagcatccg
    14161 cccaggctgc ctatttgccg gccatgaacc gcatggcgcg tctccatctt ctgccggaca
    14221 gttcactgcc ctggaatccg gttttatcct attattattg gaaccaggct ggagaaatgg
    14281 gggatgaaaa ggcggcttcc gccgcttttt ggctgttgtg gggcggctca ggcatctttt
    14341 tgctggcaat atttattatt gtctggcgtt ttcagcgttt tgccgccaga aggcttgcgg
    14401 aacagcagaa acaggaacgg gaggcctctg atgacgcatg aagcgtatta tgaggtatcc
    14461 ggcatgaaaa aaatctgtta aaaatactgt agtactccat gatgaacatg ttagctattt
    14521 ttggactggg aacaccggag attatcgcca ttctggtgat tgttttcctt ttgttcggcg
    14581 caaagaaact tccggaattt gcccgcggac tgggcaagag cctgggagaa ttcaagaagg
    14641 ccaaatctga atttgaagaa gaactgctga agaccgagaa ggagaccatg agtgatgctt
    14701 ccgcctccaa aacggaggtc cggccttctc ccgagctttc ccagcccatt gacgtggagg
    14761 tggagaagac agcggactcc aaaccggagg acaagtaatt ttttgcaatt gaacggcatt
    14821 atgaacaggg cgggaatttc ctgtcctgtt cattttagct gaaaggcttc attaagatta
    14881 ggcgggactc tttttgccgg ccgctttttc aggaggcggg ttgcaatcag aacggaatgt
    14941 tttcagaata acctgcaggt tttatttttg gcagtgatac cggagggctc tcctggatgc
    15001 gggtattccg gcgtgcatcc gtgatggagc atgtgccttg cgaaggggaa gtttttgacg
    15061 gatgcatttt tacggaaacc gcaacccggg cgggaaagaa gtgcaatttc atgttcacag
    15121 gaatactgta cagaaggagg aatggcgcag tttattggtt tttctctttt tatggcctgg
    15181 aaaaaagaaa aggcatggat tttgcggctg atgtttcccg tctttcaaag ggtatattga
    15241 atgaaaggct tcaggtattg atttcttgcg ttgcccgctg gcttgttgta cacatgtatt
    15301 aatgaaaatt catttttgcg gcgcagcggg aacgacgacg ggctcccagc atttgctgga
    15361 ggtgaacggg tgccggattc ttctggattg cggcatgtat caggggcggc gtgaggaggc
    15421 atggcatatc aataaggatt tcccctattt ttccccggcg ggggtggatg cggtagttct
    15481 gtcccacgct catattgatc attccggcaa tcttcccaat ttagtgaaac aggggtttca
    15541 gggaaatatt tacagtacat ttgcaaccag agatctttgc cagctcatgc tggcggacgc
    15601 cgcccgcatt caggagcacg actgcgcatt catcaataag atgaacaagc gccgcggtat
    15661 tcatcagccg gatgtttatc ctacttattc cgaacaggat gcggagcggt gcatgcgcca
    15721 gttcgtgaat atcgggtatg accggcccat tcccgttgtt ccgggggtga cactcacgtt
    15781 ttatgatgcg ggccatattc tgggcgctgc gcaagtgtgc ctggatattg aggacaggga
    15841 agacgggcgg aagaagcgtt tcctgttttc cggggatgtg gggcggggag ataacgaatt
    15901 actgcgcgat cccgtccctg ttccggatgt ggatattttg ctgatggaga gcacttacgg
    15961 cggccgtttc cgtgatgacg agacgttctg ccgggctatc aaggaggctc tggaattagg
    16021 aggccgcgtt tatattccgg cttttgcggt ggaacgcacc cagcagctgc tttatctgct
    16081 gaacagggcc taccatgaag gcactctgcc cttgctgccc gtatatgtgg acagtcccat
    16141 ggcagtgggt gcgacggaga ttttccgcat tcatccggag tgtttcaata aggaggttta
    16201 tgatttcctg ttccgggaga aggatccgtt ttgttttgaa cagctgcgtt tgatccgttc
    16261 cgtgggcgag tcccaggctt tgaataaaat gaacgggcag gctattatta tttccgcctc
    16321 aggaatgtgt gaagcagggc gcattcttca ccacctggcg aataacatcg gcgatccgaa
    16381 aaatacggtt ctttttgtag gctattgtgc ggaacataca cttggccgca agattatgga
    16441 tgggtggaaa gaggttccca tcttgggtaa acaatatcct gtccgggcca gaatacggga
    16501 gatggattcc ttttccggcc atgcggacca tggagaactg ctggagtatt ttgacaggac
    16561 cggggggccc aagaggaata tcattctggt gcatggggag gagaaggcta cccaggctct
    16621 ggcgggggcg ctccgggagc gtcaggccaa tcccgtttcc atcgcgcagt tgggaagcgc
    16681 catggtgctt taatgttccg gaatatagaa tttctgcagt atctgcacgc attccgggct
    16741 ttcgttctca ggttttgtgt tcaacggcgg atacatcggc agccttccaa tcaagtcatg
    16801 acgtcgggat ggctgcgctg gccgcttgac ccggtgtgtt tacggggcta gcgtgcggat
    16861 atgtcttcat tatttattga atatcccaaa tgcacgacct gccagaaagc aaagaagtgg
    16921 cttgatgagc agggaatttc ttatgaggat cgccatattg tggaacagaa tcccacggca
    16981 gaggaactgc ggagctggat tgaagggagc ggattgccgg ccaagaagtt ttttaatacc
    17041 agtggattgc tttacaagag catgaagttg aaagatcagc ttgccgacat gagcctggat
    17101 gagcagatag ccctgctggc ttccaacggc atgctggtta aacgccccat ttttattacc
    17161 aatggaaaga tatgcgtcgg cttcaaggct gacgagtgga aggccgccca tcgttcctaa
    17221 ttcccgccat gccccaaatt acccgcacca agctgggcgc tgccaatttc ggtccctggg
    17281 agaacattgt cagggtactg attctggtat ggttcatgct gtacacgttg aatgccattc
    17341 cggatattca tcctctggta ggccgtatcg cggccaaggt aaattactgg atcagctatt
    17401 tcttcatgtt tgagtatatc ctgcgggtgc tgtgcgccaa accgcggaag gcttatattt
    17461 tcagcggcat gggtattctg gattttattg tctgcgtgcc gttcctgggc atgtttttcg
    17521 ggacagactg gcagattctt tattccctgc ggattgtccg catactggct attttcaagt
    17581 tggcccggtt taatgaaacc gccgccagtt ttaaaaaggc tttttacctg atccgggatg
    17641 agttttttct gtttttctcc gtgatcctgt tcatgctgta cgtcacttcc ctgggaattt
    17701 atattgcaga gcacgatgcc caacctgaga aattccgttc cttttttgac gcgctctggt
    17761 ttgccgtgga aacgttgagt acggtcgggt acggagacct ggtgcctatt acgcccatgg
    17821 ggcgtatttt taccggggtg attatgttta ttgccgtgtc catcattgct atttccacag
    17881 ggctgatcac gtctgctttt tcccgcgtat ggcagcagga gaatgttttt gcgcaccgtc
    17941 ataagcggga cgcagacaag gtggcggcgg aggccgggga ggaggaagta cccaagtagg
    18001 cttggcccgc ttcccgtgga tttttgacac cggtttcaaa aagaacggcc gcctttccca
    18061 aagaaaggcg gccgttgcgt aagagggaca aggctgtatc ttagctgttg tacatttccg
    18121 tgtagtactg atccgccatg cggtcggagt cgaattcggg atagatgtct gtcatcgcat
    18181 tgaaaacgat gtccatccac ttgtcgttgt gatcgtagta gaggggaaga atcttgtcgt
    18241 ccagaatgtt gtagaagtta tcgcggtcgg agcggtcgcg ggcttccggg gagagatggg
    18301 cgggcgcttc cggaatgacg aagcagtttt ctccgtcctt ggcgaattca cagatccagc
    18361 cgtcattggt ggagacggag atggagccgt tcatggcggc ggacatgccg gaggtgccgg
    18421 atgcttcccg ggttaccacg gggttgttga gccatacgtc ggagccgttt ttcaggtagc
    18481 ggctcaggcc cagttcatag ccggtgagca cggcggaacg gggatatttg gccgtcaagt
    18541 cattcaggcg gttgaagatt tcaatagcgc catgatccat cgggtagggt ttgccggccc
    18601 agatgaactg caccggatag ttggtgcgct ggagcatgcg ttcgaacatc gtgggattgc
    18661 cggtgaccag gtccggacgc ttgtaggccg caaaacggcg agcccatacg atggtgaggc
    18721 agttcgggtc aaagacgcgg cccgtttgtt cacccaccat gcggaagagg gcttttttca
    18781 gagcccgctt gcggcggatg aacgcttcct tgttgcgggc ctggaaagct tccgccagtt
    18841 ccggatcctg ccagtatttc tggttctggg cattcgtgat atggatgatg gggcatatgc
    18901 cctcatagtc cttccacatc tggcgggata cttctccatg gagtttggaa acgccgttgg
    18961 cgatgtggga gaggcgcagg gcggccagcg tgtagttgaa cgtattgtcc gtcatgccta
    19021 cggcggcgcg cacctgttcc atggagagcc cgtcgaagaa ggagaacgta ttcatggtgt
    19081 ttacgtccat cttttcgttg ccggcttctt ccggggtatg ggtagtgaaa acgaaacgct
    19141 tgcgcacttc ttccacgctg cccgtcttgg caagcacgtg gaaggccgca gccaggccgt
    19201 gggcttcgtt tagatggtag atttccggtt caatgcccag ttcgtcaaaa aggcgggcgc
    19261 ctcctttgcc cagcagtacg tactgggcga tgcgggtgaa gccgttggcg tcgtacaggc
    19321 ggcgggagat attgcggctt tcctcgtcat tttcctccag gtccgtggaa aggaagaaca
    19381 tgggcgcagt gccgaatgtt tccggattca ggaagtaggc ttttacccag acgggagctc
    19441 cgcagacatg aatgaggaat ttgatgttgt gatcttccag gaacgggtag tttttacgca
    19501 tgtattgcgt ggccatggat ccgtcctccg cacggatctg gttgtagtag ccgtagctcc
    19561 acagaatgcc gacgcctacg aggttctggc gcagatcatg cgcactgcgc atatgggagc
    19621 cggacaggta gcccagaccg ccggaataga ttttaaatga attatcaatg gcgtattcca
    19681 tggagaagta cgcaacgctt ttcccatatt tggggtctat ttcatacggg tgctcgaaaa
    19741 tggtgggtaa aaatgatttc gccatggttc tgtaaataga gtaaagtgtt caaaaggaga
    19801 actcgcacgc cttggtatca tgattccttc ctgttggaaa gggcaaattg cgtgtgtgtc
    19861 tgaattgtcg attgaacagg aatgtctttc cggcgtttgt cgcccgccgc tgctccgggc
    19921 gtttccatgg gaagggaagg gggggcggaa gcctccgttc cctaggcttt ttcggcgctc
    19981 cgaaatgaat tttgagcttg ccgacgcagg ggaaaaagag catgaatggg gggcatgaaa
    20041 gctcttgtaa aaacgcaggc tggccccggt ttggaattga tggatgttcc tatgccggaa
    20101 gtcggcccga atgacgtcct gatcaaaatt cataaaacag ccatttgcgg cacggatctt
    20161 catatttgga attgggataa atgggcccag cagaccattc cggtagggat gcatgtgggc
    20221 catgagttct gcggcgtgat tgagtccgta ggttcttccg tgacggaata caagcccggg
    20281 gagattgttt ccggtgaggg gcatattgtc tgtggccatt gccgcagctg ccgttcaggg
    20341 cagaagcact tgtgtcccaa cacaaagggt gtgggagtca acaggcccgg ctgctttgcg
    20401 gagtaccttt ccattccgca ggataacgtg gtgcgcatcc acaagagcat tccgatggaa
    20461 atcgcctcca tttttgaccc gctgggcaac gccgtccata cggctttgtc ctgggatctg
    20521 gtaggcgagg acgtcctgat tacgggagcc ggggttatcg gctgcatggc tgccgccgtc
    20581 tgcaagaagg ccggagccaa gacggtggtt attacggaca tcaatgattt ccgcctgggt
    20641 cttgccaaaa cgctgggggc ggaccgaacc gtgaacgtga cccgtgaaaa gctggaagac
    20701 gtgatgaagg aactggaaat gacggaggga tttgacgtgt gcctggaaat gagcggcgct
    20761 ccgtcctgcc tgaaggacat catcgacaat tcccgcaacg gagccaacat ttccctgctg
    20821 gggattcagc ccgatggttc cagcatcgag tggaataagt tcatttggaa agggttgaag
    20881 atgaaaggca tttatggacg tgaaattttt gaaacttggc ataaaatgga ttccatgatc
    20941 cgcagtggct tgaatgtggc gcccatcatc acgcaccgtc tgccctacac ggaattccgg
    21001 gaagggtttg aagccatgaa ttcgggaaaa tccggcaagg ttgttctgga ctggattggt
    21061 tgattttccc tttttctttc catgtgatta tggatgcccc ggttttcttc caggaaaccg
    21121 gggcgttttt tagggaaggg cagtaaaagg ggggataggt ttgttgcctg aacgtcaggc
    21181 tgtttttccg ggaggctgcg gcatccgggc ttcccgggca tgggcacggc gtttttcctt
    21241 catgcggcgc gcgtagctgt cgtcatcttc ttcaaaagcg tccggatgag tcaatctgag
    21301 cctccgtatc accttgtcat ggtcaccggg ggaaaccaga tccgtaaaca ggccgccgtg
    21361 aaggtggtct acctcgtgct gcaggcagcg cgccagcagt ccgttgcatt tcacggtcac
    21421 agtccgtccg tccatcatgg ttaaaacagc ccttacgcag gagcggcgca tcacgttcgc
    21481 atatactttg gtgatgctca ggcacccttc ccggtatggc acttttttgc cgaagggatg
    21541 caggatggga ttgataaaat tcaagggcat gagtcgggac aacgtagttg ggcatccgtc
    21601 cacttccagc catgtagtgg ttgcgtctgt ggaagggatg ttgatggtga ccagctggat
    21661 gggcctgccc acctgcggcg cagccaggcc tatgcctccc tgggccaggg tttcctgcat
    21721 atcgtccagg aaggaaagga tatccctgtt gatatgaacc acgggctcgc attcctctct
    21781 caaaagagga tgtccgtatt gaagaatatc aagaatcacg gcagacagga tgatatggat
    21841 aaaagagtgt gacatcccga ttcagaagag tgttcattat attaaatgaa tattttttcc
    21901 gtcgaaggaa cggaaatgtt tttcccggat gaaatgaaaa ggccttgaac tttgatgacg
    21961 cttttttacg gtagttccgg taccgcgatg cgggccggag tttcattctc cctcctcaac
    22021 gctacgttct catgaagctt ggctcctgtt tttctctgtt cccgaatttg ccgttgatgg
    22081 gaggcgcgct cctgctggcg ctgtgtttgt gttcctgcgg agataaggaa aaggaggccg
    22141 gggctgcgga agcagggaag tcagaaaggc tttccccggt ggaatcttat gaagagctgc
    22201 tttccctgcg cctgaaggaa gtggaacaga tgacggatct ggtcgtttcc atccaggacc
    22261 gtaccgcagg agaggccctg atgaatgaac ttgggcgcct gacggagaag tttaaatttt
    22321 acgatatgaa ttgcgggcgc ctgatggctc tgaatccgcg cgtgcatgaa gaatcgcgta
    22381 agatgcatcc cgcctttcat tccattctcc gggagcgcgt ggacaaggcc cgcgatcgga
    22441 tgatgtcggg aatactgaag ctccatgaat tcaggtatta tgattccgat gtcatcagga
    22501 atgatctgga aaagtgcggt ctcagcctga cggacgaacg ggtggccctg tatttgaaca
    22561 acaagataaa gccgtttctg aaggcttatc tggaacagta ttccaccatg gtggacatgc
    22621 tggaggggat tgacaataaa gttgcggcgg aagcctatgc cgtatctgta gcccttcagg
    22681 gacgaatggt cagggagcag atgaacaaca ttgccagaat ggagaagaag tatggagact
    22741 ggattcccgg ttttgaacgg catttccatg atgggctgga aaagatgaga aagaaggcgg
    22801 aggatgagcg caaacgcgct ttccgggcgc tcctgaaact cacgacggct aggatgtatg
    22861 attccccttc catgcaaaag gcagtggagg ggctcaaaat agagcaaccc aggctaatcg
    22921 attttcagga gttctgggcc gatcctgtcg cggccatgga agagttctgc atgtgcctgg
    22981 agagaattga ctttctcctg cgcagcatca aggatgccgg aacggctgac cagtgcgcca
    23041 tggagctggt ggaagtggtg gccaagttga aaaggttgcg ctatgttgtt gatgatttcc
    23101 aaaaatccgg acgtatgaat ctggtgaaaa ccagggatat ggaacgtatc agccgccgtg
    23161 cggaggaagc ggaaatggcc gtttccgctt ccatggcccg tcttattgtg gaaacggacg
    23221 tggtaacccg ttctcctctc ctttccagag ccatggggct ttaccgcgat gtaatgtatg
    23281 cacgttaagt accgtatgcc ggaaggcgat gattgacttt cttgagtgga atatgacaat
    23341 cagcctatga aggatgttga tttttccggc agtaccgtgg tggtgacggg aggagcctgc
    23401 ggcattggcc gcgccattgc ggaagctttc gctgcggcgg gagcccgggt ggcggttttg
    23461 gatgtgaagc atgaggaaac ggcctccctg ctggaggaat tgccgggaga ggggcatctt
    23521 tgcgtgacgg gcaacgcggg tgtggaagag gacgtgcggt cttttgcgga aaaggtgttg
    23581 gaatgttttg gccgtgtgga tgtgctggta aataatgcgt gcatcacgcg tcgcggcatt
    23641 ctttccggct gttccttcga ggaattcaat gaagttttgc gcgtgggagt ttccgcgccc
    23701 tatctgctga gccttttatt tcgcgaccat ttttcccgcg gcgcttccat tgtgaatatt
    23761 gcttccaccc gcgcttccat gtcccagaag gatacggaaa gctatagtgc ggccaagggc
    23821 gccctcactt ctttaactca cgctctcgcc atgagccttt cccctttcgg cgttcgtgtg
    23881 aacagtatca gtccaggctg gattgacacg ggggtgttcg gcgtcctgtc tccggaagat
    23941 gcttttcagc atccttccgg gcgcgtcggt ttgccggaag atatcgtgaa ggccgttttc
    24001 tttctatgcg gctccagctt tgtcaatgcg gagaatctgg tcattgacgg cggcatgtcc
    24061 cgtatgatgg tgtaccacgg agatgagggg tggtcattcc atccggaaca gtcggacagc
    24121 gttccctcct gacgggcttg cgcttccggc atgctttcca ttgcagctgt ttcaaacagg
    24181 tcccgtgccg tccggacgcc tgttccgcct ggaactggaa taacagcggc aggagcgtcc
    24241 gtttgcctcc ataaaaaggg ccatccccgg aacggagtgg cccttgttta tgtcccgaag
    24301 gagaataacg tcaacggatg gtaacgcggg gatcgttcgc cagaacctcg gccagttctt
    24361 cccagccgtc ttccgtctgg agctggaaca tctggaggta gacggacgct acgccggcag
    24421 ggtatttttc gaacagttcg tccacagctt tggcgagttt ctccttgtcc agcgtttccg
    24481 gcagttcatt aacaatgccg ttgccgtcat gggggatgcc cagggtattc aggaacgtaa
    24541 tcagcatgtc ctggtgtttg cgcaacagcc agacctgaag cagatggtca ccgatttcat
    24601 tgctgggttt ccatgacagc attttcttga tgaaggaaaa ttgttcctga agaggtttgc
    24661 gctggatgaa ttcggggcgc agttttttca gggctccaag ttcacgcacg gcagcgcggt
    24721 aaatggcgcg ttcctggttg cgcatccagt ccagaatgtt gctgattgtt tcttcgtccg
    24781 cctgctggaa aatgtaatat gccttcatga taagtaggga aagaaaagct ctttgaggac
    24841 atgcactcca ccatgtttcc cccgcctatg caagctcttc tactgtcacc tgccgctctt
    24901 ggcttttatt ccgcattcaa ggcgtaaaaa aacggtctta ttgcaaaaaa gacttgctaa
    24961 aaggggttgc ttatgattac ttgctcgtgc gcttctcgcg atg
//
LOCUS       DAWEMX010000021        24801 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_814_length_24801_cov_10.484119, whole genome shotgun sequence.
ACCESSION   DAWEMX010000021 DAWEMX010000000
VERSION     DAWEMX010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 24801)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24801)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24801
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_814_length_24801_cov_10.484119"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1910..1919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10359..10368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13472..13481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17186..17195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20446..20455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5689 a         5988 c         6916 g         6158 t
ORIGIN      
        1 gtcgtacacc tctatctggt tgccctggga ggccgcggaa atgtaggttc cgtccggcag
       61 atggaaggag catccccagt tgccgtggtc ccggatcagg atgcccggca cccacagatg
      121 gttgcagttg gtgactgaga gggctttttc cgggcatccc acttcaaagc cgcccacggc
      181 gatggggcgg tcatattcca catccaggtc attgacgcgc catttgattt tggcgttcat
      241 tttctgcaca cccggctggg tgggaccgct gagttccttt ttataggagt aaacgttcca
      301 ctgtccgccg aatttggaat tgggcgtcca ggaaatggtt tccatggggg agaaggtggt
      361 cgtgtcgctg aattgcaccg tgccggcaaa gtcatatttg taggcggaat tggcttttcc
      421 ttccagttcc ttgtcgcggt gaagccccag ggcgcggccg cccagcggct gctggacatc
      481 cacggttaaa gaatattccc cgggttgggg gttatcgtcc ttgcgcttgg gttcaaagaa
      541 aatgcgcaca tccatgccca gaaccatttc cgcgcaggtg gccttggtga gctggtccac
      601 cccgggaatt tccatgatag ccttggcgat ggggagggcg cggttgttga cgacattgat
      661 aagcttaaag cggcagcctt gttccacagg aatgtttttc agttcccgga tggcggattt
      721 cagggaggcg ggcgccttgg ccccaagcgc cgcatttacc atggaccagt tgcgcacgta
      781 ggtattcagg aagcgggatt ccgtatcgat gatagggggc accttccgga atgtctttgc
      841 ctgaaattcc ggcttgcccg tgattgccag gttccggcgt tccgcgcaga gggccgtgat
      901 ccagatgctg gtgcggatgt aggtgccgat gacgggatcc gccgtcatca gttccaggcg
      961 gttggggttc tgcatcatgc cgccgtcaaa ggggcgcaac agcgtcagtt tccatcggaa
     1021 agcctccatg tttttgtaat atacttccgg attggttcgc cagtaggcgg gaagggccca
     1081 catgatggag agcgtctcgg aggagtggcc ctggtcacag ccgccgatgg ggccttcacc
     1141 gtacatgatg cggctgtttt tggtgtacag ttccgggccg ccgcagatga gggaagcaat
     1201 gtagtacggg ccatggcggc cggagtaggt tcccccgcat tcgcggttgg gttcaaaggt
     1261 gaaggtttcc gtcatgcggc cgtagggccc catgccggtg gggcagatgg actgggcatg
     1321 atccatcatt ttgtccagca catcgtcttc cgtgcgcgcc ggggttttgc tggccagcgc
     1381 caggaacagg cacatgtggg agccgccaat gctgacgcct gttccccggt agcccgggtg
     1441 aatgccgtgg ccggtgacga agtccccgta aaggacttcg tttttcatgc gctgcacgcc
     1501 gttgcggatg gccgggagaa cggagttgtc ccggtagcgc agccagtatt cacacaggaa
     1561 aatacctgcg gatgcctggg ggacggccca ccagcactgg gagccggaga aggcgatgta
     1621 gtgcgccgcc tttttaatga cggggtcata tttagggttg cccgtggcca tcagggccag
     1681 cccggcccag cccgtatcat aatggttgcc gcagtagccg cccggacgtt cccaggaacc
     1741 gtcctcgcgc tgctgggccg ccagccattc actcatcatg tggacaaccg ccttgctctt
     1801 ggcgcagttt ttgggaaagc cgagggcata ggagcccagc tggggtattt taaaggtgac
     1861 attttttacg tatttcttca cggcgtccgg cagagtggag gagcctcccn nnnnnnnnnt
     1921 cctgtccctt tgagttccca gtctcccctg gggatgtcaa aaaccaggga agagacggcg
     1981 tgggcctgcc atacgctgct tttggggtca tacttggcct ccccgtaacc tacggtgtat
     2041 tgcagggccc gggtcagcgg aacggttccg ccgggacctt ccagcctcag gccagaccat
     2101 tcaaaaccgt cgctgccaat gccgttgccg ccgtcatcca cgcgcaggcg cacctgctgg
     2161 ccgcccttta ccggaatgga aagggaaacg ggcttttgga cccgttcctc ccgtaagact
     2221 tctttccaga gaggctggat gccctggatt ttttccacgc tttccgcggg aataatattc
     2281 agggttattt ttccggctcc ttccgccttg tccaccagta ggcccgcgtg catgtccagg
     2341 ccgcgcctgt tttggtgctg ataagggccg taaagcacgt ccaggctttg agacgttttg
     2401 aaggggtgtc cgtcaatgcc caggatcagg tctccttcct tgatgtattt ttcagcgggg
     2461 gaaccctcaa tcacctgggt gaccgtgtag aggttcgcca cgggttcccc gttgggatcc
     2521 agcatggtct tggggaagaa gccgcggaag ttcgtgtccg gccaggaggc gtccaaaccg
     2581 cgggctcgga atcccagggg gccgaaggtg gtgatccagc ctttggctac ttcctcggaa
     2641 ttgccgaacc agcttgtgta caggttggcg tattgctttt tccactggtt cagcgtcatg
     2701 ggcttggcga atttcctcac ataggagttg acgtctccca tattggtggt ttgcctggag
     2761 gcttttcccc gtagacgcgt ttcaagaaca ggggtgacgg cagattgtgc cgcctgtgct
     2821 tccttccggt tggcggaatt ttccggttcc ggctgttttc cgtctgcagc gtcatgggcc
     2881 gggacatcgg attctccggc agtctgagca ggggaggcgg cggattcatt ctttaaaatg
     2941 tccggggcgt ttccgttcct ttctccggag gaaataggcg gcggtgtttt tttcggctgt
     3001 tccacgtctc cggacgggga ggcgggcgcg gcgtttttct gttccggagg ggtttccgcc
     3061 tttacggcgg gcagtacggc tttcttttcc ggtttggcag ctttcttctt ttccctgccg
     3121 gattggggga cggccggttc ctgcagagag acggattttt ccgaagttgt ctgggtgtaa
     3181 taataagttc cgcatgcgcc tcccagcagg agcagggtaa ggagccatag gccaacggag
     3241 tttttctttt tccgaggctg gggaagggaa cccgcatgga cgggcgccgc cccggttccg
     3301 ggagtggaca gcggccgttg atcgctccgg aggggctccg ccggggggat gaccggaccg
     3361 ggaggaggct ccgctgcggc atggctgccg ggaatggcat aagccaggta cagggtgccc
     3421 agggcggaaa tttcccacag ggaaccggtt cggaactggg cttcgaagtc atttttcagc
     3481 ggaagaactt cccgttcaat atccgtgttt tccggataac gcagttttcc gctttccggg
     3541 tcgcgcacag cgagcccctg ggggcagaat tcgcggagca ggtatggaac atggttggca
     3601 tccgaaacgt aataaacatg gcagagaggg gccgtctgtt tcagggattg aataagatag
     3661 ccgttgatat taaatccgga aggcaggggt ggagtatgtt ccatggtcac aggcggttaa
     3721 ttatcggcta cctactagca tacccggggg actctatcaa tgtaaaaaga ggttttcctt
     3781 ttccggagcg tgtgacgcaa ttaattgatt atttacttga atgcatgtct gttctgtggt
     3841 gcaagcaagg ctcgattttc tgtttttcca ttacaatcat gagttccgat attagattag
     3901 atgccttgca gtaccattcc cagccccgcc ccggcaaggt ggaaacgctg ccctgcaagc
     3961 cctgcttttc acaacgggat ctgactcttg cctattcccc gggcgtggcc gagccctgcc
     4021 tccgcattaa agaggatcct tctcaaagcg ccctgtacac tggtcgctcc aatctggtgg
     4081 gcgtaatcac caacggcacc gccgttctgg gactgggcaa tatcggtccg gatgcctcca
     4141 agccggtgat ggagggcaag ggcgttctgt tcaaggtgtt cgcggatatt gacgtttttg
     4201 acattgagct gaacgtgaag gaaccggaaa agctgattga gacgatcaag accatggaac
     4261 ccactttcgg cgccatcaat ctggaggaca tcaaggctcc ggaatgcttc atggtggaag
     4321 aacgcctgcg ggaggagatg aatattcccg tgtttcatga cgaccagcat ggcacggccg
     4381 tgatttccgg cgccgccctg ctgaacgccg cggagttgac gggccgcaag ctggaggata
     4441 tgaaggttgt cgtcgtggga gccggcgctg ccggcatttc ctgcgccaag ttctacatga
     4501 cgctaggggt gcgccgcgaa catatctaca tgtttgattc caaggggctg attcataccg
     4561 gacgcattga tcttcatgct acgaaagcgc agttctccca gtcggaagac tgctccctgg
     4621 aggaggccct taccggagcg gacgtgttcc tggggctgtc taccaaggga ctgctcacgc
     4681 aggacatggt gaagctcatg gctccttccc ccatcatttt cgcctgtgcg aatccggacc
     4741 cggaaattac gtatcaggat gctaaaaaag cgcggcctga ctgcattatg gggtccggcc
     4801 gttccgactg gcccaaccag gtgaacaatg tttcctgttt cccctttatt ttccgtgccg
     4861 ccctggatgt gcgcgcttcc gtcatcaatg aacagatgaa gattgccgcc gcccgcgccc
     4921 tggccgatct ggcgaaggag cccgtccccc aggaagtgat tgacctttac gggggagccc
     4981 cgctcagctt cggcatcgac tacgtgattc ccaagcccat tgatccccgc attattgaat
     5041 gggagtgccc ggcggtagcc caggcggcca tgatttccgg ggtggcccag tcccccatcc
     5101 gggatatgga agcctacacg ctggaattgc gcaagcgcat tgccgcggct cgtgaacgcg
     5161 tctcctgcgt ggtgcgcagc tatctttaac cggtttccgg cggaaggccg gcaacctcat
     5221 gcctccggcg tttgtgccgg acggctgtaa aaaccgggtg gacgtgatgt tctgcccggt
     5281 ttttttcatg ccgtgcagga acgtatttaa tggcaagggg ggagactgcg tcaaatgcct
     5341 ctggaggcga aaaaaagggg agggcggccc ggtgctttct gccgtatgaa tattaaggcg
     5401 gacatgctgt ttcatggcac tgccctgttg catgcggctt tggctgtttg tgcatcattt
     5461 catttgcctg ggcggtttcc gcagtcccgg cggactttct gccgatggga aaaatgccgg
     5521 gagaacggcg gaaggaggat gtttcccgga cgtcggagga ttgcgaccgt gatccggcgg
     5581 agaacgtcct tttctgcaaa ttaatgagga tgtggagaag gaggaattct gatacctgtt
     5641 ttacgataag tccagccacg gctcttccct gctggtatgc cgggttggag ttctggacag
     5701 aaagaaatcc tgttttttca accgcgcttg aatgttgcga gagtgaatga aaacgttgct
     5761 gtgcccacat gatagggaac accgggggct gaagttccgt accggtttgc agccctatgc
     5821 aagggaggtc gaggttcgga agacggctgg ggcagccagc tctgccaggt gaaggccctg
     5881 agcctgcgtt gggggatggg cggaatgccc aatgacccgg tacggctttt ccctccgcgc
     5941 gaaaggcagt tgggaaaatc gcagaaggcc gtagccgtac cggatattcc agtagtttgg
     6001 cggttcgagg ttatcttctt ctgcgtgaga cgagcaggcc cgcgcacgac agcgtgacaa
     6061 gcaggcatga agccggttcg gggacgcttt cggggtcggt gacggtaacg atcatgttcg
     6121 ggaagtacat gctcgtttcg ttgcccgtat tgtagtagag tatgacggga gccccgctgt
     6181 aaaaggctgt tgactgcatg gaggcgtagg caaatccgtt gtctacgctg aatggggagt
     6241 cagctccctc ttccgcctga aggctcgtgc ctattgtcag cctgcccagc atggcggcgt
     6301 cagaaacgaa ggcgaagacg tattcctgcg atgtgttcag aaggaggttg ctgaaggtaa
     6361 attcaacgtc ggtgtaggag ccgatattca cggagaggtt ttcttccgtg cgggattgcc
     6421 cgacgaccgt ccagtccgcg ctgtccgacg ccggtttggt gaataccacc atggtaaggc
     6481 tgccggatgt gaagtccgcg gtaaaccggt tgtccgtcag cttgagctgt atggagtcga
     6541 ggttcacggc tccgggcagg gcagtatcag aagtaaagcc ctggttcagt tgcatggaga
     6601 tgcccgtacc gctggcattt gcagctccgg ttcctcctcc cgtgaggatg tgggccgcgt
     6661 ttgcaggaag ggttgaaatg gccgcaaaca gggccggcag cagtaatagc agttgttttt
     6721 tactcatcat gtttcgtgtg gactttgtgt tgacgcacag cccatgcacc ggtttcctga
     6781 aaaaaggctt gccatggcgg ttgcgagacc gccggatggg aaggttccat tcattcaggg
     6841 ataccggaaa caggaaagtc cggttgagac atggaaccgg aaataacaga aggcccatga
     6901 atggggcgtt ccttatccaa agcggataat atgattctgg tgaacatggt ctatcaaatg
     6961 gtttcccgcc atatgcatag gcatccatca ggctggtatt ctggcttatc ttcctgtaga
     7021 agaattacag tagcggaact gctccggatt ctaaccggat tccccatcta tttcctgacg
     7081 gctcccgccg tcgtcccaat ggagattcat ttttcatgaa tcgccgttaa tgtagtctct
     7141 tcccttcgtc tggcaagaaa gaaatttccc tatggttaga tgcaggaaat gttattttta
     7201 tctggcattt tcaatccatt tgggaagaat cgcaggatat ggccgggcaa aaggccggct
     7261 tcctttcgtt tttggtgaag gcatgtctac acctgggcgt tggccagccg gaggcggatc
     7321 tgttccgtct gctccctgtc cgggatgagc tctacaagcg tctggctgtt ttcttccagg
     7381 gaatcaagct ggtcgaagtc ctccgccgtg cggatggtca ggtagcgcat tccccattgt
     7441 tccgcaatgg ccttgaagga atgggcgtgg ggttgtacaa gcagcttcct catggtttcc
     7501 ggctggccgt ctgctcccgg gagggtgagg aagatgcccc ctccgccgtt gttgatgacg
     7561 ccgaggacgc gggtccccct gtccagttgg ggaaggagtg ccagggcatt ggaatcatac
     7621 atggcggtca ggtcgcccac gagagcccag gagcgggaac accgggcgga gacccccaga
     7681 aaaccggaga tttgcccgtc aatgccgttg gctccccggt tggcgcggac gttttccgtg
     7741 gggatggatg tctgggcgaa gctgttccag taccgcacgg gcatggagtt gcccaggtaa
     7801 atgcagtctc cgtccgcggc aaagcaggag aaggagcgca ccatggcctg ttcgctttcc
     7861 gggcaggtga tgagcagttc ctccatcagg gctttcctgc gtttgttcat ggcgcgcagg
     7921 ccgttgacgt cgcccacggt atctatatcc cccagcgcat gcagaatggc ttccaggtct
     7981 ccggttacga cggaggaggg gcgggccagc ccggagaagc cggtgcgggt gacggagaag
     8041 acttccgtgg ctggaatgtc ctccaggtcg cgccagaagc ggcccacggg cacgtcccct
     8101 aatctgagca ggacggcggg cgggtgttcc ctcagcaggg cgtctgcgtc cgtcagagcc
     8161 agatgggcca gctcttcccg gagtccggag gtggcgtccg ccaccacggg ggctttcagt
     8221 tcgttggcca gccaccgggc cggggcttgt tccgtggggt ccaggccgcc gatcatgacc
     8281 accagcccgt cgcgcgattt gaagcgcagg gcccgggcca gttctgccag ggagccccgg
     8341 aatccgtttt cctccggagg ttccgccggg taaaggtcgc agctgccggg gttccaggca
     8401 gggtccggat ccggcaggca cacgttgata tggagggggg aggcgtaatc ccagtcttgc
     8461 agaatgtctt ccggcaggct ttccggcgtt tccaggtcaa ttgtgggtgc gtaaatgccg
     8521 aacagatccg cctgttcgat ggcctggggt gcggcggaac cccggcaggc cgccgggcgg
     8581 tccgccgtga gcaggagcag ggggcggcgc tggtagtagg cctccaccac ggcggggagc
     8641 agttccgccg ctgccgttcc ggaggtagtg accacggcca cgggcagccc catatcctga
     8701 atgcggccca gcgcaaagaa ggcggcggac cgttcgtcaa agtgagtcca tttcaccaga
     8761 tcctccgcgg cagccagcac ctgaagcagc gccatgttgc gcgcgccggg gcagaccacc
     8821 cactcgcaaa tgccgcccag gcagcattgg gccagcaggg atttgacgaa ggatgcggag
     8881 gaattcacgg gaatcagcct tgtttttttg ccgccctggc cgctttttca gccagtttgg
     8941 ccttttcctc ttccgaaaga atgcggggaa aaacgggggc gggatcattg atgcggtgcc
     9001 ctgcggggag gattccccac tccagcgcgg ccggcgtaag gccccggaac aggtccccgg
     9061 cgttgagctg gcctagaatg cgggcggagg cgtcagggag gacgcagccg atgagatagc
     9121 ccgtttgggc gcagcattcc agcaaatggc gcagcacgca gcggaggcgc ggggcggagg
     9181 cttcatcctt ggccagctgc cagggctgct gctgctcaat gtacgcgtta caggcggaaa
     9241 cctgggcgtt cagggccgcc agggcatcgg aggccatgtt gtcgtccatg tatttggaaa
     9301 acagggtgac ggcctggtcc atggtggcgc gcagggcttt ggaggcttcg tcatcatatg
     9361 cctccgcgcc ggagataacg gagtcgcaat agcggcgcgt catgttgatg gagcggttgc
     9421 acaggttgcc gaggtcgttg gccagctccg tattgtacag catgaccagg cggtccgcat
     9481 caaagttggc gtccttcccg gtggtgatgt cccgcaccag gtaatagcgc acaggttccg
     9541 ggccgaattg ttccgccagc aggttggggt ccaccacgtt gccgatggac ttggacattt
     9601 tttccccgtt gatgttccac cagccgtgca cgagcagggt gggcatttcc tcgtcggaaa
     9661 agcccatggc atgcagcatg gcgggccagt acacgccgtg ggcgggcacc aggatatcct
     9721 tgccgatgac ttcgcagtcc gcgggccaga gtttggagaa ttccggcagg ccgctgtccg
     9781 gctccgccag gtagccggcg aaggaaatgt agttgatcag ggcgtcaaac cagacgtagg
     9841 tgacgaattc ggggtcgaac gggaattcga ttccccagga aaggcggctc ttggggcggg
     9901 aaatgcagag gtccgagtca aagtcaatgg cgttgagcac ttccgccttg cggaaggacg
     9961 gaaggacaaa atcactgctc ttttccacga actgcttcat ccattccaca tggtcggtca
    10021 ggcggaaata ccagttttct tcctccagtt ccaccacttc cccccattcc gggccgaagt
    10081 tgccttccgc gtcccgttcc ttatccgtca ggaactgttc ctggcgcacg gagtagaatc
    10141 ccttgtagga ttttttgtaa agctgcccct tgtccttcag gtccgtcagg attttctgca
    10201 cgcaggcctt gtggcgcgga tccgtcgtgg aggcccatcc gtcattgctg atgcccaggc
    10261 ggttccacaa cgccaggaat tgctccgtga tattgttgac atgctcctgc ggcgtaatgc
    10321 ccagcttttc cgccgtctgc tgcactttct gtccatgcnn nnnnnnnnca atggccgtgg
    10381 tgatatagta catgcggggg acgatactcc tttcctgccg ttttgcaagc aaagtcatgg
    10441 cggcatcccg ggcgtgtttt tcagcatggg atgggagagt aagggggtgt cgcgggcgaa
    10501 acggcggaag gggttgtgcg gatattccct ttccaccgcg ccgcagcgtt ctccgcaagg
    10561 gactcaacag gagaaaatac ggcggggctg gatgtccccg ggaaaatttc cccaatacat
    10621 ggttccggaa atgaattcca ttgccgggga gcgtgatgcg tgtctttaat tttgtgattg
    10681 ttgttgccgg aagccgcggt tttcataggc ttcctccagc tggctgagcg cccgtaccag
    10741 gatggagcgg ggacaacccg cgttcaggcg catgaagcct tccccttcct tgccgaagat
    10801 ggaaccgtcg ttgagagcca ggcgcgctcc gtccacaaaa aattccacca gttccttctg
    10861 ggaaaggttc atttcccggc agtccagaaa taccaggtag gaggcatccg gcatgatggc
    10921 cttgattttt ggcatgcggc gacgcagttc cgcgtcaatg aagcggatgt tttcctgaat
    10981 gtagtccagc gcctgggcca gccattcctc cccttccggg cctgaatagg cgctgatgag
    11041 cccgtcgtac gcgaaaacgt gcccttccgc aagctccctg ccgctgacaa agtcttggta
    11101 tttcgcgaac aggtccggat tctggcaaat cacgtaggaa ctgcccaggc cgggcacgtt
    11161 gaaggtcttg ctgggcgcca tgtaggtgac ggagttcatg cgggcgtgtt cggaaacagt
    11221 ggcgaatacg gtgtgcctgt ggccgggaag ggtcagatcc gcatggattt catcagaaac
    11281 gaccagcgtg ccgtttgccg tacagatttc agccacgcag cgcaattcct ccggactcca
    11341 tacgcggcct ccggggttgt ggggattgca caggatgaac atgcggcaac cacgcacggc
    11401 ttccttcatg tggttgaagt ccatgcggta gcggccgttt tccagaatga ggggattgtt
    11461 gacgatttcc cggccgtttt cccggatgat catggcaaag gggtggtaca ccggaggctg
    11521 gatcatgatt ttgtcccccg gttccgtaaa gcattggatg gcgctggaaa tgccgggaac
    11581 aatgcccgga gcgaagccta tttccgaggc gttcaccttc cacccgtggc gtcggtccac
    11641 ccagtccctg atggcttcaa agaaagcgcg gggcttgacg gtatatccca ggatttcgtg
    11701 ctcgcagcgg cggcggatag cttccatgat gaagggaggc gtcctgaagt ccatgtctgc
    11761 cacccacagg ggaatcaggt cgtcccggcc ccagcgggga agcagcgctt catatttcag
    11821 ggctccggtg ccgcggcgct caattacttc gtcaaaatcg tactgcatgg aaggtctgtc
    11881 tggcggtcaa tggtcaggaa agaggggtaa cgcctttttt caggatgatg ttgccgtatt
    11941 tggccgtttc cccggtaatg acgacggcat aggccttttt ggaccgttca taaaaagcgt
    12001 agcgctccat ttgctcaatt tccccgtcat agttcagtgc cttgcggtat ttggcttcca
    12061 ccgcagggtc cagagagtcc cccggaaccg gggccatcat gatgacgggc gttgcgtagg
    12121 catccagttc gaagagggga atgacggcag ccagcagggc atccgccccc aggccgtccg
    12181 ccctcagcac cgtagggttg aaggtatggc cggggaagtg ggcgtctgaa aagacgattt
    12241 cgtcgccatg ccccatttcc gcaagaattt tcagcaggga agggctgatg aggggagaaa
    12301 tattttttaa catgccccgc agtatagcac cagcccgcgt tccggtacta tcataaaatt
    12361 ggggtaatgc cgttcatgcc ctctcagccg ttaacagtct ggaagcattt ccggacgcat
    12421 tcttccattt cgggcatttg ctcttccatg ctttccacca gcctgagctg ggtgcgggca
    12481 cggtgcaggt tgtgccgttc ttttttcgtt ttgaaataaa cgtccccttc caggtagtcc
    12541 gttaggaagc ggattcccgt ttccagcgtg ataagcagac cggaaaaagc aagtttggag
    12601 acttcctgcg gcgtgatgaa ttctctggag gcctccaggt atcccttgac gacggcctcg
    12661 aacatgggca tgcgcaggaa ggttttatcc agattttctt catcttccgc tgcaggggag
    12721 gtcatggtgc gtaccatgtc tccgaagtcg tacaggacgc tcccgggcat gacggtgtcc
    12781 aggtcaatga cgcagacagc cttgtccgtc tccctgtcca gcatcacgtt gttgattttc
    12841 gtgtcgttgt ggacgattct gacggggagt tccccccgtt cctggagatc cagcagcacg
    12901 tccacataac gttcacggcg gcggatgaag tccagctcct tgcggcaggt attcagccgt
    12961 ccgtgggagt ctgctgccgc ggctttttcc aaatggtcaa agcgcctgcg agtatggtgg
    13021 aagaacggaa tggtttcatg gatgtcctcc ggattcatgt cacacaggag ctgttggaaa
    13081 gcgccgaaag cgcgggctgc ttcgtaagcc tggcggggat ggtccgccac gtcgaacgtg
    13141 tgggtgtttt ctatgcagtt gtagcagcgc cagaagccgg aaccgggaat ttccaggtat
    13201 ttgcggccgc cccgcgcgga gtacaggttc agcgtctggc ggaagggcgt tttcagcacc
    13261 cggaacattt tccagcggat gtggttggtc accttttcca cgttgtgcat gacatccctg
    13321 gggcatggga acacggcatc gttcacgcgc tggaagatgt agcggcgggt ggtgccgtcc
    13381 ggcttcctgt aggtggcccg gaaggtcatg ttgatatggc cgctgttaat gacgtcgcaa
    13441 tggaggaatt ctcctgcgat ggcgaatgcg tnnnnnnnnn nccggcgacg aaggtgaaat
    13501 tgcggtaaac cttggagggc tccctgtagg ggaaacgggc ggaagcgccg cggtggttgg
    13561 ccgccaggcc gccgttgccg agccagaccg tacccacgaa cggagtcacg gtcaggtctt
    13621 tcgtggcctt gacaggggcg gaaaccgtca gggaaatgcc ctgggttcca ttggcgttca
    13681 gggaattgct gtcaaaatag ccggcagtgg cgttccaggt gcccgccacg atgacggagg
    13741 tggatttgtt gacgtcatac ttgtacccca gagtattggt gaaccaccag ccagtcatcc
    13801 agcggaacgt atattgtacg cggcagtccc agaagaagtt ttccagccct ttgccgaagt
    13861 catggtgaat gtccagaacg aagctgtgtt cttccggcct attgtggctg aaagcaggga
    13921 agccggagtt gttgcctgta ttgtggatgt tgaaacttcc ggggataccg ccgtggacaa
    13981 actggtaact tagagccgcg acagtggatt ttccgaattt gcggctgacg cctaaaatgg
    14041 ccgagccttc gtcgcaaatg ccggaatcat tgtgttccag accattatcc gtcagccact
    14101 gatattttaa tccgcctatc agggaatatt gattgttcag ttgataggcg ccgacggctt
    14161 ccaggggaat gacatgatca cttgcggaat tctggaaagc cagaccgcgg ctgatgtatt
    14221 tggtagcgta gcctgctccc agctgggcgg agaatgtctt gccgccgtta ttggcgttta
    14281 aaacatccag tggaagctgg atatcggcag tagccatgcc ggaaaaagcg gccatggccg
    14341 cacatgtagc aataatggtt ttatgttgca taattagagg ggaaaaatat aataacaata
    14401 tttttatttt tagcaagtgt atcttcaata ccagaaaaga aatatgaaaa tatgttcaat
    14461 gtatgacaaa aagtgttaaa catttgacgc attattcatc atgcaagggc attttttccc
    14521 aaagagaact ctatagcttc cacaaaagcc ggtttaattt aaatgtttcc ccgccggagc
    14581 tgcggcagac gtgttcacgg ctatggcttt tgtaaacctg atgatgccgt ggattctttt
    14641 ttcagcgctt cctttaactg gggtttcagg tgctggaaga gtttgatgga gcctttgctg
    14701 gacatgtggt cgctatcctt gtacagaatt tccccgtgtt cataggcgtt gaacggcctg
    14761 tcttccggta tatagggaag tatgctcagg acggtgcaga gcccttcctg ttcaagacgg
    14821 tcaaggatgg gaagcacggc ggcatgatct tccctgtatt tttgccgcgt gcaggtaagg
    14881 ctttccaggc cttttcccgt ttcccccctg cgtatgaggt tcctgatgta tttggccacg
    14941 tcattttgct tgatgagcgg agtgggggcc agcaggatga cttgcttgcc catcgcccgc
    15001 agttcggaga taaatgcacg gagggagttt gaataggcag gttcgccgga gggaagcggc
    15061 ttcagcttcc agtccattac atttttactg tcgtaacggg cgctccagtg ctgggctatc
    15121 accacatggc tgatttccgg gtggacgttc agccatgcca ttagggcctt tatttttttc
    15181 tcgtcgcaat agtagcttgc atcaaacggc agcgcgggca attccctgtt ccagaatggg
    15241 gaaatgacgg aagaaacgta aatcccggag atatccaatt ccctgcatgc tgcgtccaat
    15301 ccgcaaaagg ccgcagaggc gtggctgtcc cccagcagga ctatggatgg agcttcggca
    15361 ggggaaccca gctgcagcag gccgggttcc gcacttccct tgaaatcggc tctgctgacg
    15421 aaggcaaaga tgttgttatt gaatttgatg gcttccttat caaatccccg ggcggcttca
    15481 ggagatttga tttccgatgc gccatgcagc tgggggctgg aaatggagtt gatttcctgg
    15541 ttgatgtagt tccggaatcc ttccgtctgc cttcctgcaa cgcatagaag caacgcgaaa
    15601 aggtacaggg ggataaacca tttcatcttc atgcgctttt tctctactgc aaggtacagg
    15661 agacctccca ggactgcgca cgccgctccg atggcaagca ttccgggcca tccggggaca
    15721 tggagaaacc acgaatgata gaacgcgatg aggggcatat gcaccaggta aagggagaac
    15781 gagattcctc caatccacag cagaagttta ttgttgaacc agtgggccag cctgccggat
    15841 tccgcatacc ggatcaccag gcatgtcccc aaaactacgg ccgccgagca gagggagacg
    15901 tcctttccgc gggaaagggc aggtacgagg atgcataaca ttccaaggat gaagaggaga
    15961 gcgtttttct tgctgttttc gcaacgtgtt gaacttaaag gtggggggta tattgctaac
    16021 aaaatccccg cgcaaacctc ccacaaacgg ggcagcgtca tgtaatggga agtggggcgg
    16081 acctgctccc agacgggcag gccgatttcc tgcaacaggt tgtgaatggg ataggaatag
    16141 gcccagagaa aggacccgat gccggcgatc cagaaaagag aggcggccgc ccgtttggga
    16201 aggtaacggt acaggacgca tccccccgcg aacagaaggt aaatctgcgc cgtcaccgcc
    16261 aggtaccaca tgtgaaggaa tggattgccg gaggaatcct gcgcgaaata gtcggactgg
    16321 atgacggcca ggaacctgtt tgcggcgcct atcagcgtat atccgcctgt ggagctggct
    16381 gtcgccagat ccgtatagtc cagaaagaac atgccagcca gaagagtgag cgggacgatg
    16441 acggcaaccg gggtaaaaag tcttaagacc tttttacggg caaattcccc ggggtggatt
    16501 tgcatctgct gtttctggag ggatttcatg agcaggtagc cggaaatcac cagaaacact
    16561 tcaacgccga agtagccgta ggggaaatgt tccggattga ggtggaaaag aatgatcaga
    16621 ataatcgcga gtcctctgag tgaagtgatt tgcttcatgt taattaagat gtactttaaa
    16681 tttgaaatat atttgtgaag ggatgctgaa tgctgtccat tgttttttcc actgacggca
    16741 taactataaa ataactctca tgccgagccg gaagcaggat ggaacgttag tacttcatcc
    16801 ggcagatggg cggcctgatt tctaatcaaa agattgataa aaaattgagg aattatgcaa
    16861 aaatagtctt ggcgcaagat tttcaaaatg ttttagatat tgatatttag caaattgctc
    16921 cgatgaaaaa tcggaaagat caacgaaaca agactaaagc aaataatgcc gaaaaattat
    16981 gatgcgatat ttatttctgt ttttttaaaa aggaataaat tttttcgtca acgatagttc
    17041 tataccacca tccttggagg aaatgccata aaaagccttc tttcccatcc aaaaagccca
    17101 gtcttaaaaa ataacggtaa caaaagtaga aaaaagagcg ccagaaaaga ggaatgcgtg
    17161 cataaatatt tttttgtctg cgtttnnnnn nnnnncaaga tgcgtgcctc tctcttcgcg
    17221 tagtttaagt gtttggttat ccaccattcc aggttattga gattgttgtc ggcgaattct
    17281 atgtccgtat ctttgattat tccttcctga accagcatat gttcatccat gaggcgattc
    17341 tcatagcggg cttttcccgt acgaaacatc cgtactaaac ggatagtgcc tgtaccgtgt
    17401 ttgatgcgtt ttcccaaaaa ataacggacc aggatgaaag ataaggcatt tacctctgga
    17461 gtaagatcag ccgtaatttt ttttaatttc tgcaggcttt ccggcattaa atattcgtct
    17521 gcatccagtc tgagaatcca ttctgtttta acaggacagt tatttaacgc ccagttgaat
    17581 tgttctgctt ggttgccggg ccattcgtgc tgaataacat gaacctgttt atattctttg
    17641 caaatattta tcgttgcgtc tgtggaaaaa caatcaatga cgaatatttt ttctgcaacg
    17701 gaaagaagat tctcaatgca tctgcgaatg tgaatttctt cattataagt cagaataatc
    17761 gcggaaatat tcatgagttt taatgtctcc ctcctgttaa aatagaatat gcggttcctg
    17821 tatttttctg taatatgcgg gcgatgacga tacttgccaa gaggatgatg agaggcgttg
    17881 tgaagtagcc aaacaaataa agaagtttca ggggaggaag gaagagtgta attgcctttt
    17941 tataaatatt tatccacggt tcatggaata cgtatataaa aaatgtgtaa ggagcccaag
    18001 aaattatttt atctaaccag tttttcttgg cggaaaataa atcatataac gtccatactg
    18061 aataagcgcc aagagtcaac ctgagcatat tcagaaaagg caactgaaac tccggataaa
    18121 aacagttgac agcgcaccat atgagataaa gccccgaagg gataaaaata gcgtttttgt
    18181 tctgttgccg ttcaatattg atgccgttat aggaaataaa accccccatt aaaaaagcaa
    18241 gcaatccctg catgttttct cccccccatc cgtaaattcc tatagttacg cagaggatga
    18301 gaaatatgac gacagtggcc catttgctat ggaataaaac ataaaatatg ggacagaaca
    18361 gaacaaaaag aatcaggtat tgaagaaacc acaaatggaa agcggccggc tcccagaaaa
    18421 tgaaaagaaa taattccggg atactttttg tttgaagcag agtcagaaaa tccgaattga
    18481 tttttgatcc aatgtcgggg atgagctgaa gaaaaataat ggaaaggaaa aataacagat
    18541 tccataataa atagggaagt atcagggagt gtgccctcgt acgcattttc ctcagtattt
    18601 tctgtattcc ccccccgtct tttgtctctc ggaaaaacag gtatccggag atgaaaaaaa
    18661 acaaaggaat acaaaatctt acgatttgac agccaatcca atcttgaata aatgcattga
    18721 tttcccaggc attgttttcc acaaaataac tgtgagcgac cagaacccca atgatgctga
    18781 taaaagagag aatttttaat tttttactga actccggatt catgaaaatg agtaagaagg
    18841 gaagtttgtc caatacatct tttttaagaa accctgccgg atctttttag tggcaccatt
    18901 ttttattttt gcggaagaaa tgatcgagga ttcggcagct gcaaccgtgt ttttcaatag
    18961 caatatacct gacagaaccg tgtctaccgg cacaatcctg aggcagcctt taatcatggt
    19021 aggttttacg attagttaga aattcggaga atagatattg tgagtgccct cggagcatgt
    19081 ttatattgca accgatgcga acggatttgc cacgttggat aaatctcgta cagtaaatgt
    19141 gacttctcaa ggccgaagaa gaaaaagaac ccacagtcat cgttttagga ctaattgatg
    19201 atgtattata catgcttacg gagcaagtta agcccactat ggttccaaaa aggcaggtaa
    19261 gaaaaaacgg aattttttag aaaatatgga aatatcggga ttgaaattcg gttgacgggg
    19321 gagtacatgg tgtttaagag ttttttccta aaaggagaaa gctttattgg aaaaggtgga
    19381 taatcagtca tgattattga ctgttcttgt tttaataaat tttgccggat ttcctccaac
    19441 aaccgtccat gattctacgt ttttgaatac ggcggctctt gcgcctacca cggcgccctg
    19501 accaatgttt actcccatac ccacaaaggt atcggcggct aaccatacgt tattgccaat
    19561 cattatcgga gcggtttgta ggggcagcat taaactggaa atatcgtgcg aagctgtgca
    19621 caaacaactt ccctgtgaaa caacagaatc atctcctact gtaatcatat ctttactata
    19681 aagatttgtg tcaggtccaa tgcaagccct tcctattttc acgttccagg gagcaaagat
    19741 ggtacatgat gagtagatat atgcatcctt atctattgaa gcgccaaaac aacggagcaa
    19801 tgcgatgcga acagggttaa atagtcttcc ctttagacat ctgaataagg tatggttgat
    19861 aaggtaccat atggctcttc gagctaaatg gggtttatat tctttatatt ggctaaggtt
    19921 catcgcttca ttcttagggt tcggtaaata gggttcagat acttcttcat gaatcgtgcg
    19981 atacatgaaa ccagtatgat actcgtccta actaatagag acatgcccag tacctctttt
    20041 ctagctctgt cagcctctgt caaacctaac gcttggttgt tgttggaaat accggtaatg
    20101 tcaaaatccg atatacacat ggggacttgt aaaatatttc ctttggcata tttcactact
    20161 tcgagattta atttataatc agccgcaatt tgataactca agtcatactt aagtcctttc
    20221 tcctttaaaa actggcaatt ataaaaaata gattggtgcg aagcgaaagc tccataccat
    20281 atttttttat aactccgtga aggtattatg ggtgacttac cttctacgga agattcccga
    20341 taatcgccat aaaccagcgc cggaaaatac ccggaagact tgatgtaatt tacgattttg
    20401 cttacaactg actgatcgct aaagcagtcc ccactattca taaatnnnnn nnnnntaatt
    20461 acctttcacc gcttgtaaac ctttattcat tgcatcataa atacctttat ccggttctga
    20521 gatcaaggtt cctaaaaacg cttcatagcg atatatgcat tctttagtgc catcagtgga
    20581 agcgccatct acaattacat agtctatgta agggtagtct tggtccaaga cactgtgtat
    20641 agtacgttca atgtggttat gatcattata tactatggta actatagata taattatctt
    20701 tttcatggtt acttcttttc cgtattaagt ggcggtctct tgatttttgc ttaatgcatg
    20761 gaggtattgc gataaatcaa ttttgtttca ttcatataca aattccggtt tactgcattc
    20821 tcccaataac catttataaa gtgtagccat catttcagcc accttatcag ccgcatattt
    20881 atcagaaaca agcctcttgc cacgaatact catagaggct atttccttat cagatagatg
    20941 caaagccttt tctatagtga tggctatatt atctactgtt gcgtctatcc accatccaca
    21001 gtgatgagta tttaaatctt tccatggagt ggtcgtgttg gctattacag gagtgcctac
    21061 agacaaagcc tcagtaacag tcatgccaaa attttcaaaa tcactaacaa gacacagaac
    21121 cttcaaattg gccagttcct ggaatttggc cttaccattt acaaacccat gaaaagtaat
    21181 gtgaccatat ttacattcag ctgcaagacc ctttaaatac ttctcatatt ctggcgtccc
    21241 ttcacccatc agtatcagtt ctgcatccgc cactttgtta cctaattgtt tccaggcttt
    21301 aatgagttta tctgcctttt taactggatg cagtcgtcct agaaaaccta tgcgttttag
    21361 tgaatggttt tcaataatgg tgtcaatcca tttcacagga ggggtaggat taggaattac
    21421 agctataggt cctttataac caaaggttcg gataaactcc atttctttct ggcatgtggc
    21481 atgaagacat gtagcggaag cgatatcgtt cctgtggcct aaggaaagaa tgagtttttt
    21541 tctccagaag cttctttcca gagcccgggg ataaagcatg ccatgagagg aaagaacgca
    21601 gggtttgcgg aattttctgg ccgcggcgca ggtggcgtgg ttaatgtcca gccataggcc
    21661 attggtatgg tagagatcat attgctgatt ctgctgtaaa aaatgtctac aatggcggga
    21721 tatttgaaga ggcgtttttg cgtcaaaagg gacggtttta ataaaagggg cctgaataga
    21781 agaggtggcc ctgttcccag aagcaatggt cagaatgtcg gttcggcatc ctagagcgtt
    21841 caaaccagtg gctaagtcga aggtgcaggt tgttggacct cccgctttag cgtccataga
    21901 agcgatggta tgtaaaatgt tcatagagtc atattttatc ttgccaaggt ggtaatggtc
    21961 gggacgatgt tttaccctga atgaatgaga ggatggaagt aagatagcag gccggagcct
    22021 gccctgatat ggttgttttt gcccaggagg caatgcctgt tcgttctttt tgcgtaattg
    22081 gccctttact tatccaatgg cgcagctccc gttccaggct gcctttttca ttccatgaaa
    22141 atgttcctcc cctatgtatg ccgtccagca ggcatgacgc gccgcaacga tcggaacata
    22201 aaacgcgcat gccttgctgg agcgcttcat tgatgacggc gccccatccg tcatacatgc
    22261 tgggaagaat aaggaggtca tgggactgca tgatgcgcag gacattttca tttgcctgtt
    22321 ttccaaggta acggaagttg ccttcttcat gaatgaggga ttccgtttct ttgaccagag
    22381 gccctgtacc aatgacggta aaagtgtcaa tttgaaagcg gaacgatttt aatgcatgaa
    22441 tcaaatggag gattctcttg ttgttgtcca gccttcctac gtacaggagt ctgggggctg
    22501 ttcggctgtt ttgttggtcg gtattttgtt catctgcgga atgtttcgtt tgttctgtga
    22561 agtagcccca ttcaaatatt ctggatgcag gccatccgga gcgccggtag gcttgcacgc
    22621 cgaattttcc cgttgccagc aaggcttgaa ttttctttcc gtattttaaa taaagaagag
    22681 cgtatttaag ccggcgcagt tggccggaaa tacctcctgt ctgatagggt tccatgtaga
    22741 tgagcgtagt ccttttcagc tggatgcatt gtttgaatgc tcggaaaacc atgggatagg
    22801 catctattcc tgaaaagacg tggatggcgg tttcattgga aagaatcagt tgatgaatac
    22861 gctgttcatc aggagatagt tcaagatgga catttcccat atccgggatt ttccatccac
    22921 tgtccgtcct ttcctcttca aaagctcttt ggacggccag cgttacctgg tgttttctgg
    22981 ccaaggcatt gagaagagtt gtttgatgga tgctgatcat attttgccag aagaggaatt
    23041 tcataggcat gcaatgttag tgatattttt attctgccat gcggaagcgg ctcctacgga
    23101 aagccataga agggcgcaga gagggcttcc cgccgcaaag acatagcctt caatcatcat
    23161 atgcattcca aagaatacaa gacagacttg ggcaggcgaa atgacatgca ttccgaatgc
    23221 agctgacggt tttttcagga tctgactggc aagaatcatt aaaaatggga ttccacccaa
    23281 gaggccggtc atggcgaaga cagctccata ggaggtccca ggttccacgg ctccggtatt
    23341 cagagtccgt atatcaattc gtatttcttt ttgggaagag aagccaactc cgtatatggg
    23401 agaagacttg aattccttaa ttcgttcgtt ccataaactt tgtcgtgagg ataaagttcc
    23461 gcctttttcc atattggctt gctgcttgtt ttgcaagccg tcagcaaagt tgccgataag
    23521 gggcgatgct gctgtgaagc atagaagaag cacgaagatg attttgggag ctttgcgtat
    23581 atggtcttta gtaaacatga acaggacgta caatgccgtc atcaccgtgg cgaggaaagc
    23641 gccccgggaa gatgagagaa gcatggaaca caaacaggtt cccagcatta gcagaatgag
    23701 cggagagaaa gtggtttgag agaaatatcg tttcaagatt ttgtataaaa gccaattcgc
    23761 agcaatgccg gcaaccggcc caagaagcat ggattgcggc gtaattccgc caaatgaacc
    23821 tgaaaaggaa tcattaacgt tgatgattgt tctcatgtaa ttgatgccta ggaagtagca
    23881 gaagaaggag gctactccga caaagacaca taggttcatg caccattcaa aggcttggat
    23941 gcggaatata tccaattgct ttgatgaaac cagagggctg actgtcaaga ggaggagaat
    24001 gaaatatacc agccgttgcc atggcttgaa aatagcagcg ggatctgcca gaataatgct
    24061 cgcggaacac gcagcgagta aaaaaattat aagaaaattg atgttggtta tttttcttag
    24121 agcagaaatg ttcaaaggaa gaaccagcag agctgcggta taaagaaaag aaaattgatt
    24181 cagcggtccc agaagggaag tggatatggc cgccatgaat aaaaatgccc atgcgccgtg
    24241 ctgtgaaata tatttaaaaa tagtttgcat tcaggatatg ttttttattg gtttggccgg
    24301 aactcctgct gcgatgcaca ggcttcctat gtttcctgtt actacggctc cagcggcgat
    24361 gacgctttta cggccgattc tggttcccgg caggagtatg ctgccggtgc cgatccagac
    24421 tccatcttca ataaccacat ctttggaaat accctgtcca gccgttcttt cccctaccgg
    24481 gtcaactatg tgcgttcctg taccaatata gactttgggg gcaatatcta ctttggcgcc
    24541 tacggaaatc agggatgtgg agaccagcat gctttcatgc cctatccagg ttccttcttt
    24601 aagaataata tggcccgcac ccagaaacct agcagaggag cagacacgaa catcatgccc
    24661 tatttcgtag ccaaccagac ggagaagact tctcttaatg gagaagcatc ttgtttccgg
    24721 gagcaatttt aataaaaata gaaggaatgc ttttttcata ttttaatcaa gaataaagcg
    24781 aaaaaaacgt gaataatcct t
//
LOCUS       DAWEMX010000022        24762 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_816_length_24762_cov_13.162100, whole genome shotgun sequence.
ACCESSION   DAWEMX010000022 DAWEMX010000000
VERSION     DAWEMX010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 24762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24762
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_816_length_24762_cov_13.162100"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    19448..19457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5602 a         7705 c         6655 g         4790 t
ORIGIN      
        1 tcccttgaca aaatccgtcc cgtcgtaaag gcagatttcc ttttccggca cgttcaccac
       61 cacatggaaa tccagaggcc ggtacttgaa tacatctccc gcatgaagag cgggaatatc
      121 catcaaaccg ttcaggtgaa ccaccatggc ctgagagcaa cccagttttc tgaccatgcg
      181 tatccaggaa tctcccttcc gtacggtata ttttttcacg tccggtccgt tgggatccag
      241 caactggcgg atgcgtagcc ttccgatgag ttccttcgcc tctttcccca ccacgcctcc
      301 ctgttccgcc agaggctgca gcagttccag agcttttggc agactgccgc ggtccatcag
      361 gcggcgggct tcagcaaggg cctccggacg gcgcggccag agggaataat tttctctgtc
      421 cgccaaaact ccctgggcac agaaagccac cgccaaaagg ctaaagaaga cctttgcgct
      481 tgaagtcaaa gatattgatt tcatgggatt tttgtatcat ttccaccgtg aatttcaaga
      541 gggagacctc ccatgcgtca tccactccct caatgagagc catgaatgta tcttctccat
      601 tgcgcacctg gtccagcagc cggccgcgca cggaatcaag cggttcatgc agaatttcct
      661 cgctcacctc cgaccgtttg aacctgaacc cgtattccag cagcgtcaaa aagcctggcc
      721 cgtttttgga gagccagtca aaaaagcgct tggcttctcc ggaaaacccc tccgtactga
      781 tttcattgta aatggaaggc cgtatgattt tcggtttctc cgcttccacc cagccgctgc
      841 gcacgcgcac cacgttcacc tcatccatca gttcgctgag gagaagaaaa ttaaacctgg
      901 tatcgccaaa agtatcaatg cggcggtccg gttcatacac cacccgtgtc atctccgccg
      961 catagcggat atcgtcacgg gaaaaggaag gcaggtcgct catggaagaa aaggcggaaa
     1021 ggctttccat gaaaaagcgg cttccttgcc ttggacaggg aagccgcttt tgtaataaag
     1081 gatttttagc agtttaactt aaccctgaac cttttcgatg taagcaacga cgtcggcaac
     1141 agactgaagc ttttcagctt cttcgtcggg cacttcgatg tcaaagtttt cttcgaaagc
     1201 catcacgagt tcaacggtat ccagcgaatc agcaccgaga tcctcgatga atttcgcatc
     1261 agcagtcact ttatcggatt caacgccaag ttggtcgacg atgatacttc ttactttttc
     1321 ttcgatgctg ttgtcagaca tgatatttat tgttaaggtt cattgatact acatggggta
     1381 tgcgccattt gcaacaccta tttatgagtt tttatgcgtc atccggccac ggagccggaa
     1441 gcagcaggtt tccagccgat tttggccgtt ctttaaagat tcagtccggc ggctatgcgc
     1501 aagccgttca acgtaatgtc agggtccaca tggtcaatgc gctccactcc cctggcgatc
     1561 aaagctgcat cccctcccgt agcaaccacg gccggacgca cgcccagctc accttccagc
     1621 cgggccagaa tttccttcac cagcccccgg taaccgtaca cggcgccgga ttgcatggcc
     1681 tctactgtgc tggttccaat gacacgggac ggctcctggg ggtcaatggc gggaagaagc
     1741 gccgtattgc gctccaggta atcccccatg ctggcaagcc ccggcgcaat aacgccgccc
     1801 atgtaggtgc attggggccc gatcacgtca aaagtcacgg cggtgccaaa gtccaccaca
     1861 acgcaaggat acccgtaata ggccgcggct cctacggcat tcgccagcct gtcggcccct
     1921 atctggcgcg ggtggggata gtcgatgccc acgcccaacc gggaatcgca ggaaaggaaa
     1981 tggcacggtt tgacgatcca gtttttcagt tcttccgcca cgcgcggcac gacggaacac
     2041 accacggcgg catcataacg aacgccggcc agcacctcgt ccagccgttc cccgctcacc
     2101 tcccgggtgg gaaccatata ccgttccggt aacagggctt ccggcgaaga gagcacgaat
     2161 ttggtgcgtg tgttggaatt gtcaatcaga agataggtca cgggaatcag ttagtatgtt
     2221 cctccgccag tttcaaggcc aaatccgcta ttctgccgct tcctccggtc atcccggccc
     2281 gcagcaggtt ttcatgcatg cgcctccact gtgcgccgcc gtccgccatc atttcctgaa
     2341 gagcggaagc cagggcacgg gcgtccggca catggctgcc gcctcccagt ttttccagga
     2401 ggcgggtatt gccctcctcc tggcccggaa gaagaaagtt caccagcatg ggacgtgcgg
     2461 ccgcaagcac ctcgtgtacg gtggctcctc ccgctttccc gaccactacg tgactggcgg
     2521 caagatagga aggcacgcgg cgcgtccagc cgcgcaccat cagcctgcgt ccgtatctgg
     2581 cgcgcaaatc ccggatgcgg ggataaatgc gccggaaccg gcgccccaga atacaggtta
     2641 catgcagggc gggattcgca tccagcatac ccgccagctc cgcccgcgca tgccgtgcgg
     2701 aacgctgggc aaagtaaagc acccggaacg gttccccttc cttccaggaa aggggatgtt
     2761 cctccgccag cgctcccagc acgggattga cgggaaatcc cgtaacgcgc agcctgtcct
     2821 gcggaagccc cttttcctcc ataattgccc tcgtccaggg gtccgttacg gcccagaggg
     2881 gggacttgga acacagccat gatttattga tgacgaagga atccgtcacg acggtcaggt
     2941 aaggaaccgc cctgcccgtg cgggaggcca gggaatccag catgtaggga tataccatgt
     3001 aggtgcagac cacggcatcc ggcttccatt cccgcagcaa acgttccagc aggcgctcca
     3061 cgggggcaat tcccctgacg ggcttctccg ccatattgcg cctgtcgctc acctcgtaca
     3121 tccatttcca gaggcggggc atgcgggaga tcgtccacag gtaggcggcg cgcgtcaggc
     3181 ggaatatccg gggcatcgcc tccgcgcaca ggtccgtaac gcggacttcc gcgcgccctg
     3241 cgagagcatc cctcacgccc ctggctgcgg aattgtgccc ttccccgtat cctgcggtca
     3301 caatcagaat gcgcggcagc cgtcctttct gcatagaaca gggtatacca atattccgct
     3361 ccgtcgaaaa gaacttcttg caggagggag gacatgaggg gaagcggccg gaaaaaacat
     3421 tatcctccgg tattccctcc gaacggaaag gaacgggaaa aaacggcctt tcttccctat
     3481 tattcccttc tcaaaaccgg atttaacatt gacctgcgca ggccgtcagg gcaagcttcc
     3541 agccatgcgc atcgtcttca tgggcacagg cgacattgcc attcccgcct tccgcagcct
     3601 gatccgtcac tcggacctgg caggcctggt cacgcagccc gaccgccctg tcggcaggca
     3661 ccaggtgctt acagccccgg ccatcaaaaa tattgccagg gaagcgggca ttcccgtgct
     3721 ccagccgcat tccctgcgca gcccggatgc gctgagcaac ctcaggagac tgaatccgga
     3781 tctgatcgtc gtcatggcgt acgggcagat tctgagccag gaagtgattg atatggcgcc
     3841 catgggctgc atcaacgccc atgcctccct cctgccgcgc caccgtggag cggcctgcat
     3901 ccagtccgcc atcaaatccg gcgatgcaga aacgggcatc accatcatgc acattgtcag
     3961 gaagctggat gccggagaca tcatcgccca gatcagcacc cccctggaag gctcggaaac
     4021 cggaggcacc ctgcatgaca agctggccag gatgacgccg gatgtcctgc tgcccgtcat
     4081 ccattccatt gaaaaaggga cggccacgcg catccgccag caggaaatcc tggcaacata
     4141 cgcccccaag ctcctgcggg cggacggaaa aatcgactgg acgcgccccg cagaagaaat
     4201 cggccgcatg atccgggcgt acgacccgtg gcccggtacc ttcaccaact actggaaccg
     4261 caaaaaacgc atccgcaaca tgaaaatttt ccccggcttt tccatcctcc cggaagcgga
     4321 ggggaaaccg ggccaggtgc tctccgccgg ggaacagggg cttttgatcg cctgcggaag
     4381 cggaggattg ctggtgacag acgtccagct tgaaggcagc acgcgcatga atatctccca
     4441 gctcattgcc ggccacccga atttgaaaga tatccatttt gacgtataaa tggccaagtc
     4501 cttttctatg gagagcctga acgcggccca gaggaaggcc gtgcagaccc tgcaggggcc
     4561 cgtgctgatc ctggccgggg ccggaacggg taaaacgcgc accgtcacct gccgcatcgc
     4621 ccatatggtg gaccgtggca tcagccccaa aagcatcctg gccgtgacgt tcaccaacaa
     4681 ggccgctcta gaaatgcggg aacgcgtagg ccagatggtg gaacgcaagg cggcgcgcca
     4741 aataatggtg agcactttcc attccctttg cgttcgtatc ctgagagagg atataggacg
     4801 cctggggtac aaaaccaatt tcaccattta cagcggttcc gagcaaagcg gcctgatccg
     4861 gcgactgatt gtgcgccacg gcggcattac ggagaaaatc acgcccaaag atgtgctttc
     4921 ttccatgagc cggatgaaaa acagcggtct gggccttgac tccattgaag acaacctgac
     4981 agccaacatc gccgcctcct accagcggga acttcaggcg cagaattccg tggactttga
     5041 cgacctgctg attctggcgg acaaattgct gaaggagcac cgggacgtca gggaggcgtg
     5101 gcaacagcgc ttccgctata tcaccgtgga tgaattccag gataccaaca gcctccaaat
     5161 gagcctgctg ggccatctgg taggtccgga acacaatgtc tgcgtagtgg gagacgacga
     5221 ccagtccatc tacggctggc gaggcgccca gatcagcaat atcctggaat tcgaacgttt
     5281 tttcccaaat ccctccgtca tcaaactgga agaaaattac cgttgcaccg cccccatcct
     5341 ggacacggcc aatgccctga tccgccacaa cctgggccgg agggacaaga cgctgcgcgc
     5401 ccacaaaggc ggaggagaag ccgtccggct catttccatg cccggagacg cggaagaggc
     5461 ggaattcatc attacggaca ttgaaaacgt ccgcaggcag gaaggccggc cctgggagga
     5521 tttcgccatt ctgttccggg ccaataccca gagccgcatc attgaacaaa cgctccggga
     5581 acacaaaatt ccctaccgca tggtgggcgc gcaaagtttt ttcgaccgca aggaggtgaa
     5641 agacctcatt tcctatctgg cgactatcga aaacccgcag gctgacgaat acctgctgcg
     5701 catcctcaac acgccgccgc gcggcatcag cgagctgacc agccatctgg ccatcgactg
     5761 gagccgggag cacggcaaca gcgtctgggc agccctccag gacgaagaat tcctggcggc
     5821 tctttccacc cgcgcccgca acagcgtgca ggcattcaat gaactgattg caaaatacat
     5881 tgaccttttc caggataagg aaaccgactt cggagacatc ctggagcagc ttattgagga
     5941 aaccggattc agcgaatacg tcacgcgcct gtgcaaaacg gaagctgaaa tccagaaacg
     6001 gctggtgtcc atcggggacg tgaaagcctc cctccgcaac ttctggcaac ccggaaaaac
     6061 cctccgggac tacctggccc aggtcaccct ggacaaggag gataatgatg acgacgtgga
     6121 aaacaagcca ggcgtatgcc tgattaccat gcacgccgcc aagggactgg aatttccggt
     6181 agtgtacctc gtggggctgg aggaaggcat tcttcctcac aagcgttccc tggaagacgg
     6241 caattgtgac gaagaacgcc gcctgcttta cgtaggcatc acgcgcgccc aggagaaact
     6301 catgcttact tattgcgcca cgcgcctgcg ctacggggac cgcatgcctt gccagcgctc
     6361 ctccttcctt agtgaaattc ctccccacct gatggaatat tccaaatggg aggatctcat
     6421 gaacgcggaa gcgacggaag aggaatccgg aaactttttc gattccctgc gcagcatgct
     6481 tctggaagag gaataagagg cggtatggcg ttttgccggc ctcttttcaa ccatgtattt
     6541 tattcagaga acaaggcgcc gtttccgtcc ggaaacgcca gtctgccggc tttccgcctc
     6601 cagaatattc agccgcgcca gctgcatggt ctgttccgga tgtgaaaaat cctcccggcc
     6661 tgaaccggga ggattgaaaa tacgggactg gaggtcttcc ctggattacc agaagataat
     6721 gtacagggcg accgtcgcgg caaccacgag ccatccgaag attttagccc gtgaagagga
     6781 ctgaagggca accacgcccc tgtcaggcag cacgataggc tgtccgccgc gggcggcgtt
     6841 caccgccgtg aagatgaagc cgatgaccac taccgtgatg aaacacacgg ccatgcggtt
     6901 caggaaggcc atttccggct gccatacctt caggattccg tacaggaggg cattaatgac
     6961 gatacccagc caaccgaaga tgcgcgggca cttgggcaca aagaagccga agaggaacac
     7021 gcacagggcg cccgggctca ggaagccctg gaattcctga atgaagttga agatgccgcc
     7081 aaaggccggg ctgtccagga acggagcaat ggtcagggcg atcacggcgc acaccagcaa
     7141 accaatcttg ccgacggtaa ccagctccgt gggccccgca tttttgcgca gcttgttgta
     7201 aatatccatc gtaaagatgg tggacgcgga attcaacatg gatgccaggg aagacaccac
     7261 cgctccaaag agggccgcaa gcacaaacca tgtccagccc gtgccgggaa gcaacttcct
     7321 gatcaacgtg ccgaaggcgg agtcatagtc aaagcctacc agcgtatccg tcagatagta
     7381 gttatccgag ttgtccacgg ccggcttgat gattttgttg ttaagggaag cgattttttc
     7441 cacgaacggg gcgcgttccg ccagcgtagt ctggtcgttg gcggcatccg ccttcaaatc
     7501 ggcgcaggcc tgtttcaaat tggccataac atcttcgccc accttcatta cttccgcgtt
     7561 atgaatggca tgggcacagc cttcctccac gttttccacc aggaagctgt ccgtaagctt
     7621 gtagatgacg gggcggccct tgactgcggc gggatcttcc gtcttacgga tttccgccgc
     7681 gtattttact tccgcctgtt ccttcaggtc attgcggtac aggttatagg ccaggatgcc
     7741 ggggaccacc accacgaatg ggatgatgag cttgaggaat gcggcgaaca caatgcccat
     7801 ctgaccttcc gccagggatt tggaagccag cgtacgctgc atgatgtact ggttgaggcc
     7861 ccagtaaaag aagttgggaa tccacaggcc caggcaaaga gccgtccacg ggatttccgc
     7921 atcatccgca ggacggatca tgtgcagctt gccgcccacg gtatttacgc cgctggccgc
     7981 gtcaccggag ttcagctcaa aaaagcgcgt caccccatgc cagaggctgt ccgaaggatt
     8041 ccccagggag gcgaccgtcg cgccggaatt ggcggcggcg gattgaatca aatggttcgg
     8101 gtctgcgccg ctgagctccg tcagggcaaa ataggccacc acgccgccgc ccacaatcag
     8161 ggcggcgccc cagaacaggt ccgtccaggc gcaggctttc aacccgccca cgaacacgta
     8221 taccgtggcg cagaaggcaa tgataacgca gccgacggtg atgtcgccga agtcaatgcc
     8281 catggcagag tatcccgtga agaacacaga aatcaccttg gcgccggcgt aaatcacgcc
     8341 cgctgtcggc acgcctacca gaatcagcag cgtagcaatg gccatcaggg aacgggccag
     8401 cgtgttatac cggtattcca ggaattcggg aatcgtatat acgccgcctt tcaacagctt
     8461 ggggaggaag cagaaggcta caaccaccag cgtgatagcg gccagccatt catacccggc
     8521 aatggccatg cccacccagt tggccgattt gccggacatc cccacaaact gttccgtgga
     8581 aatgttggcg gcaatcaggg agaaacccac cagccaccag gtcaaaccgc ggccggcaag
     8641 gaaatagtct gaagctccct tggccttctt ttcctcttcc gtttcttcgg ggtcacggct
     8701 cttccagatg cccagggcga tcacgcccac cacgaccacg cagaagagaa tgacttccac
     8761 catggaaaga ggggccgttt taggctccgc agaggcggtc tgttcagcct gtacagcctc
     8821 tgcggcggga gccgccggct gaatttccgc tgccggctgg acttcaacag cgggaatcgc
     8881 ggccggagcg gccagggcgg gctgcgcatc agctgccgtt gcaggtacgg gaccctcctg
     8941 ggcaactgcg ccccacgtca tcagggcgag cgccccgaat atggttgcta gtgttttcat
     9001 catcagtatg gattaagctt ggtacagaac acgggcgcct tctccggcac gggttacaag
     9061 atacgtagtt tcaatgcccg tttcctggca gtagccttcc agaagctcct gggccacctt
     9121 ttccacatcc tccgtcttca ccagggccac aatgcatccg ccaaacccgc cgcccgtcat
     9181 gcgcgcgccg taaatggagg atgcggaggg aatgcggtca caaagggaaa caagggtatc
     9241 cacctcagca caggaaactt catagtcgtc ccgcagggaa gcatggctgc cgcgcatggc
     9301 tacgccggcc tcatcccaat cgccgcggga aagggcgttc gcaaaagcgt tcacacgcgc
     9361 attttctcca atgacgtggc gggcgcggcg atagcgcaca tcgccaagct gttccctgaa
     9421 ggattccagc agctccagcg tagcttcccg cagggagggt acgcccatga tggaagaagc
     9481 ttcctcacac tgcttgcggc gctgtccata agctccatcc gccagggaat gcttcacagc
     9541 gctgtccaga accagcacgg atacggactc attattcatc ggaaccagct catattccag
     9601 cgtggcgcaa tccagcttga gagccatgcc cttcttgccg ttggcggaaa taaactggtc
     9661 catgatgccg cagggaacgt tcacgaattc atgttccacg gcctgcccga tgagggcgac
     9721 ttccttggga tctatttcaa cgccggcaaa agcggcgagc gccgtacaga cggcaacttc
     9781 aagagcggca ctggaggaga ggccgccgcc gcggggcaca ttgctgtcaa tcagcatatc
     9841 cacaggcccg atttctatgc cgcgcctgtg caggttggcc aaaacgccgc ggacataatt
     9901 ggaccagaac ggttcgccgg gaaccaaggc gtcttccact gcaatttcat ggatctggtc
     9961 acccagggaa ccgcaaaagc ggtgtttgcc cacgggggag ggagccacag ccacaacaca
    10021 atggttatca agcgccatag gcatcacaaa accgttatta taatccgtgt gctcaccaat
    10081 aaggttcaca cgtcccggtg ccgctgccac atgagtaggc gcctgaccga aatactcgat
    10141 gaaatacgga gtcaccgttt ctttagagat ttctcgctga actaaatcca tagtagattg
    10201 attctgcccc atccctttcc gcacctcaag attaaagtct ctgtccggca ataattttct
    10261 tcattttcca cagaatacac caattccatc cctctcccga attgcagtat tcttaaaaca
    10321 ggcctgttcc accggacaga gaaccccagc cggattccaa attcccggca ataacggctg
    10381 acggcctgta atccagggag accaggaagc tactttgccc cacgggcgaa aagcactgcc
    10441 gggacggttt tcagaagcag tttgatatcc agccacaggg accagttgtc aatatacttc
    10501 atatccaatt ccaccagctt ttcaaattcc cggatgttgc tgcgtccggc aatctgccag
    10561 taccccgtca cgccgggcag cacgctcaac ctgcggcggt gggcatcgct ggtaaaggct
    10621 tccgtttcat aaacgggcag cgggcgcggg ccgaccaggc tcatttcccc tttcagcaca
    10681 ttaatgagct ggggaagctc gtcgatgctg aatttgcgca ggaagcgccc gaaggagaaa
    10741 atgcgagggt cgtgctccag cttaaagatg gggccgtcca tgtcattgcc gtactgggct
    10801 ttcacttcat ccagcctttt atccgcatcc cggtacatgg tgcggaattt ccacatgcca
    10861 aactcccggc catacagccc ggaacgtttc tggcggtaaa aaaccgggcc gcgatccgac
    10921 agcttgattc ccacagccgc cagcacccac aggggtgacg ttcccaccag aagcgccagc
    10981 gctcccgtcc agtccagcat tttcttcaac tgccaggagg tggtataggg aatgggggtg
    11041 gatttgtaca ccagcattct tgttcctcca atcacgccga aataggtttt atgcacggcg
    11101 ggcatgctta ccaccatgct cgcgtaaaaa tcgactccca tcttgccgca cagttccacc
    11161 aggcaccgcg ccacggaaga atccacctcc tccggcagaa acaccacctt gctgacgcag
    11221 ttcttaatca gcaaattcct gactaccgca ctgtccacct cttcaggcgc catgaccagc
    11281 agcccattcc tgaagtccac atgattgctc tccagcgtcc tgcggaattc tctcacatgg
    11341 tccggagttc ccaccaccag cgtaggggct tcctccacca gccttttttc cgcccggaac
    11401 cggctgatac gcagccagag ggaacccagg aaggtaagca gatacgccag ggactggaca
    11461 ccgacatccg cggaaaaggg aacaaagaac ttccatatca tcacgcttaa caccacaaaa
    11521 caggcatatg ccaccacaaa ccgccggatg ctttcccaga gcgtctggca gcggtagtaa
    11581 acgccgaact ggtcacagca cacatacagc agcagagaca tcgtcagcgg cgtcagcacc
    11641 agcgtccgca gacgccacgg ttccggagcg accccatccc attgccagga cacaaatccg
    11701 acaacgaaga aaacgacgaa cgcaaggacg aaaacaacaa tcttatttat taatcccgta
    11761 ggtagtacgg tctgcatttt gaatcagcat cgagagaata tgtagattag tcggcattta
    11821 aaatgctccg tcctcgtctt tttgtcaaat tgtttgctca cgctgagcca tgcccggcac
    11881 ggaagcctgc ctcccggacg gccttcccgc ctccgggcgg tcggaaagcc taaaatgacg
    11941 agataccggc ttgcaagaat ccggctccct gtgcattggt atacgcgtca atcgaccgga
    12001 ctggtcacgc gcctagcctg atcgcccccc tatcagacca tgcatggacc gttgatcggg
    12061 gcatggggcc cccttactcc tggcttttct catggcagcg gaacgtcccg gcgatcctcc
    12121 ggatggaatt tactgcacac agcgataaat ccggcatgac accaacataa tcaccaactt
    12181 agcattataa gtatgtcaca acacaacaca cagggaagac agggccaggg agctcgccgc
    12241 cgctacaaca agggcggacg ctcctatggc aatgaccgcc gcgattccag cggttaccgt
    12301 cccaagtaca gaaaagaagc gcccgccaaa ctctccttgt ggcagcgttt cctgggcctc
    12361 ttcggcattc gtccgtccaa gaataacaag catgcggcca gggataaagc cgccgccaaa
    12421 accaacaccc gcgtagcccg caacaggcaa gatcgcgttt ccgcacccaa acagcccgtc
    12481 tccaaccgcc ggctttatgt gggcaatctt tcctatgaag ctacggaaag cgacctggag
    12541 gacgtgttca agggcatcgg ggaagtcaat tccgtggaaa tcatttacaa cccccgcacc
    12601 cacaagtcca aggggtatgc ttttgtggaa atgaagaaga tggaagacgc catccgttcc
    12661 gtggacattc tccacaacca gcctttcatg ggccgcaatc tgctggtcag cggagccaat
    12721 gaacgccagg aacaaccccg gcagccccgc gaagaccgcg cgcccaggga agaacaggat
    12781 tccatggcgg aagtgaaaga aacctccact cccctgctcc agccggaagc tcccgctccc
    12841 cagtcccagg aacaataacc gttttaccat gcggaagccg gcctttcttc aaaggccggc
    12901 ttcctgcatt tacgggaaag ggaactccag gcagcaacga acgtgcagca cggcacggga
    12961 gccgcaccat ccaacagccc tccatccgta gggtttaaac cactcccctg tccgtcccgg
    13021 ggcggcggaa caggcgtgcc tgcaaaggcg gattctgaaa atatcacggc agcccccagg
    13081 ctctgcgcag gcgcaaatat tccctggcgg ggagcaggat ataaagggga tgtctggaag
    13141 gatccagcca tgcaatcatg gccctcaaat tttcctcgct cattgcggtg catccggccg
    13201 taggggctcc tccgtcccgg cgccagatat ggaaaaaaat ggaggaaccc gcacccggca
    13261 cgggtttctg ccgtccgtct gcggcattat gccggataaa cagtttcaga ctgtgggcgt
    13321 aatcattcag cttcatctgc tgcttgaact cccaaggcgt gacgggatca tgcttcagca
    13381 caaagtgctg gttatacagg ggagaggcag ggtcatccac ccacatgtcc cgcggcgtaa
    13441 tgcggcggta cggcatggaa cgctttttct gcggcgcggg aacagttcca tatacgcccc
    13501 ccagttcaaa aattcccgcg ggagagcgca agtccccctc cttcttcacc ggccctccgg
    13561 cgggagggag gctgacgccc ctgccccaga ccagaccgga ttttcccaga cgcgccggaa
    13621 acggcccctt caccatcacc cagccccgcg gccccttctc caaaaggctc aactgcacat
    13681 gggaactatt ccacccgtcc gcggaaccta caatcacctg gctgcaatcc gcagggagcg
    13741 ccgcagccgg aaaacagaac gccgccagca tgcacaaaca caggtatact gccttcatga
    13801 gccgtagcat aacggcaccg ttccggaaga aaagcaatta ctccgtctcc gcaggcgcgc
    13861 cgtccggcgg aatccacccg cctagttcaa cggaagaact tcttccagca gggactcaat
    13921 tccctccttg gcaaaccggg aatacagata cagccttttg gcttccatgt atttgcccag
    13981 ggcagacccc acttctcccg cattcctgca tttttccgca tcattcagca gattcaccag
    14041 tgtgcttgcc tttgtcgtca ggtccagata ccgggcattc agctcctggt cattgatgaa
    14101 cagttcctgg tccctggaac gcagctgttg aagctcctcc caggcgccgt atacgtcctg
    14161 catggcttca cgctggcgcg ctgcgcccag ggcacgctca ataacggcca ttttgcccag
    14221 atcttccaca aactgcttct ggagctccgg atcatccttc accagaacgg caaattcatg
    14281 ctggttgtca aaaatgcggc gataattctt attcttgtgg aaacgcttga aatcctccag
    14341 agcgtgggat tgctgacctg cggccatcat ggcgttccgc agcggaagaa gacggggatt
    14401 cttcggccag atttcaatgg cacttttcag gcattcctcc aatttggcgc ggtcccccct
    14461 ggccagggct tcctgcccct gggaaatata ggcgtcactg gcgaatacgg cggcgctgat
    14521 agcggactcc gcacggcccg tactgaaatc cctggaagtc ttcttaaggt tctccaccag
    14581 ggaaagggct tcttcgtaat ctttgccgtt catcaactcg aacaactttt ccacatcccg
    14641 tttgaaggca tacagttttt gcttcttctc atacggaaac gtattcacgg cgggcatgaa
    14701 ttccccgatc aggagagctt cgttcagccg ttccagcgca tccacgtaat gatgggaatc
    14761 caccaggttg ttcacggcct tcatgtgccg gtcggagtca cgggccagct cccgggaaag
    14821 gttgtccagg atagcgacgg tgggaggagc gcccagcgta ccgctgaaca tcttggcggc
    14881 atcggaattc ttatccagcc gcagcttggt atccccgtca ttgaacaggc ggctgtaaac
    14941 gccgcagcct atggaaacat ggtcaaaacg ccgctgggca aacaattgaa ccagcgtaga
    15001 ctgaaaaaca atcttcgctt ccgtcaccgt cagaacctgc tcactttcca gcttcagctt
    15061 gcgggcttcc atctccacta cacgcttttc cagatatttg taacgggggt tatttttggc
    15121 cggatgattg gtggatgttc cctttttccc gcccttctgg ccggagggcg caccggaaat
    15181 cgtcgtcacg ccgctttcca tcaggctcat cttctgggcg ttgctgcgga tttccctttc
    15241 caccgcctca agcatcttcc ccttgttttt tccattgctc ctggcctgcc acgcggtgta
    15301 aatggcctgg cagagagcgt cggaaacgcc tccgtccagc gggttgctgg aagcggcggt
    15361 caacaggtcc actacctgcc gcaccacatt atccgccacc agtttttcct tgcgcatgga
    15421 gagcgtttcg tggagctttt caaaaatttt ctgatattcc ttcgcatctt ccgtactttc
    15481 ctccggctgg ctcaggtact tgttgaaacg cgcttcaatc tcccggaaac tgcccatatt
    15541 caacgtcgtt ccgttgatgg tcaccgtgcc gtcctgaggg ttcaggaaag gaagctcatt
    15601 gccgtacagg gacttggcat ccccctgcct ggaaggctgg taggcattcg gctgaacggc
    15661 gggaggggag gcaggggacg cggcaggcgc accgccggct gcaggagggt tgtacatcat
    15721 ctgccccagg gcgggaaccg cgcaggccag ggtcatcaaa aaacagcatt tcataggatg
    15781 agggagattg tttatttagg ctgaatggat gtagctacgc acacgccgcg caccttcgga
    15841 ttgatatctt tcacccggaa ctctatcctg tattgctggt cccggttgat gttcagtccg
    15901 ttcctggcct cagcggaaac acagacggga ataatgtact cacgcccgga ggcgtctttg
    15961 tacaagaagg caataagctc ggactcgccg cgcggaaaac ggtcaatcac cgttccgtcc
    16021 agtgcatagt tgttattgga aagctgggag gcgttcgccg ccagaatgtc cagaggtata
    16081 tccgacccgg cctgcggctt ctccccggct cggaacatga caatccccac aataacggcc
    16141 agcacaaaaa ccgccgcccc gaccgtcaaa accagtgcgg atgatgagga acttttcttt
    16201 tttgctgcac gccgtctcgc cataatttct gatggaaaaa gtatataacg tacttcccgc
    16261 aagcgcacgc attaaattaa agctcccagc gcgaacggcg gattcccgcg taggcggccg
    16321 caatgccgca cgcaacatgc acgcccagaa caatatagca aaccagggag ggagtcaggt
    16381 ccgtatccag cacattcttg acggcgtaat atacgtcgtc cttcagtgcg gagatgctgc
    16441 cggaccacgc ccagtacgcg gaaatgaagg ggcgtatgaa accctccgca tattccggaa
    16501 gcgccagcac cgctccggag agaggaagct ggaaacccac cagataaatg ctcagcaggg
    16561 aagcctggtc cgcactccgg gccagggcgg aaatgcccag gcatacaaaa gtcatggcgg
    16621 cgttcgccaa aatcaggaac agcagatgcg tcaggccgcc gccacggtcc cagaaaaaat
    16681 ccacaaaggc aaacatccag acggattgca ccagcaccag aacgctcaga taggacagct
    16741 tgctcgccag gtaggcggaa ggcctcatgc ccgcatactt ttccttttcc atgacggcgc
    16801 gttcaccggc cacctcgcgg gcggcattgt tggaccccat cagccccagc aaaatcacct
    16861 caaacatgat gatgccggag acggcggacc ccgttttcat gttcagctgc tgggcttcca
    16921 tatccttctg gacagtctgg acatcctgcg tggcggaaag ccccacgatt tgcccggagc
    16981 ccttgtccgt aaacatggct accagcacgg gaaacagcag caccatgacc aattgaagaa
    17041 ccaactggga acggtcgcgg aaaaagatgc gccagcgccg ccccagcaga caaaagaact
    17101 gggtgaagaa tccgggaact tccggaatgt tttcctccac ggccttttcc cgttccgttc
    17161 cggattctcc ggaggacccc tcctcctccg cctccgcagg acggacggca tccggatccg
    17221 gcagttcccc ggaaagaatt ttcttttccc tttcctgttc cagacgggaa taataggaat
    17281 cccggtgctt gctccaggac cggctccagg aagggccttc gcgatcctgg agcttcgggt
    17341 aaatctcctc cagcgaggaa acgccgaagt aatgcagcat ggttttcgga gaaccgtggt
    17401 aagccacgca gccctggtgc atgaccagga tggaatcata cctgtccaga tgggaaaggc
    17461 tgtgcgtgac ggaaatgacg atgcggcctt ccgaacggga gatttcatgc aggaggaaca
    17521 cgatatcgtg ttccgacctg ggatccagtc cggacgtcac ctcatcgcaa atcagcagcc
    17581 ggggattgga taccagttcc atggccagcg ccaggcgcct tttctggccg ccggaaagaa
    17641 ttttcacatc ccggtccgta atcccctcca ttcccgttac ctccagggca ttgtcaatgc
    17701 tgtcgtccaa atcgtccacg gaattgaacc ggcaacggag cctggcggcg ctctccacat
    17761 tctcatccac gctcagctgg tcataagcaa tgctgaactg gggaacgaac ccgatttccg
    17821 aaggttcaaa gtcttcttca gccaaatcat ggccattcca gaaaaaacgc ccgcccgttt
    17881 ccgcaatcat gcccgcaatc gccttcaata aggtcgtctt tccgcagccg gaaggaccaa
    17941 cgaccgccat aaaatgaccg cgaggaacat taaaactgac attgtccaat aatttcaaag
    18001 gaccatccgg tgtttctatc tcatacgtaa tgccgtccgc ctgcaacatg gttcttttac
    18061 tttaacgtat caacttccgc atcatcaagg aaaatcgggc atttgcactc tatcccgcca
    18121 gcaacggaga acatgctctc cggaaacctt ccctgccagc cgcgggaacg ctcccgcggt
    18181 caggctcctc ctgcccccgc tcatctcggc atcccccgcg gccgtatccg tgcgcaaacc
    18241 gggctgcacg gaattccccc gaaaaaccgg atggacagac accgccctcc caaaagggag
    18301 gtttgaagcc tccgggagcg tttatttcat catcttccaa accgccagcc gttttcaaaa
    18361 cggaaaatcc gcatccccca ccggatccac agctttgtcc tcaaactttt taacagcttt
    18421 ctgctgggga tttacggcac ggaccaccag cgcccggggg ggcatttccc gccgccttcc
    18481 gaactcgcat ttttcctcgc acagatcgca ggagttccgg cgttcgcgtt cctcaggccg
    18541 ttccgcggta acgggacagg cgtgttcgca cgcgccgcag ccgatgcaca gggaaggatc
    18601 cagcctgggc aggcggtgct cctggccgtc acgcccgata tagggcaccg tgtaaagggc
    18661 cttggtgggg caatgctccg tacaggctcc gcaatccatg tcctcccggg caaccaggca
    18721 gcggcagacg taaaaccggg ccacggcaat gcgcgtagta tcttttgtta ttccctcagt
    18781 atccctttcc gcggtaacag gcatcctgcg tatggcccct gtggggcaga cttccgcaca
    18841 gcggtggcaa tcataaaggc agtatttggt ggcaaaatcc atccggggtt tcatgaatcc
    18901 cttcaggcca agctgcaggt atgagggctg aagcacatgg gtggggcagt tggcaacaca
    18961 catctggcag ccggtgcaga tatccagaaa acgttccagg gaaccggctc cgggaggaag
    19021 aacgcactgg gtggggtcca gcttccgggc ggcatctccc cggcatccgg aaagggcggc
    19081 cgtggccagc ccggccgcag tagcctccag gaactggcgg cgggaactcc cggcaatgga
    19141 gggaaccgcg gcggaagaag agggagcggg agccggggga gccttctttt tttgggccgg
    19201 aaccagttgg cgggccctgg taaaccagcg gtagcgtatt cccccctcac tgcaggagcg
    19261 cacgcaatcg tagcacgcca cgcagcgtga ggagtctatt ttgtattcct tcaggttcag
    19321 gcattgcgcc ttgcaggatt tgacgcattt gccgcatttc ttgcaggagg cgggatcaaa
    19381 gcccagccgg aaaagggaga tacgggaaag caggccaagc agcgtgccga cggggcatac
    19441 cgtattgnnn nnnnnnnttg caatacaggc gtcccttcca ggcactcatg acggccacca
    19501 ggccgagcat gccggcggaa gccagcagaa ccgggaaagc cgcggggaaa taatccatct
    19561 ggaccaggtc cgcagaagaa ctgtccgccg ccagcaggtt attgctccaa atggcggccg
    19621 gccatgccag cacatacatg atgcgcccga aaatgctgta agggtccagc caattcagag
    19681 acagaccggc cagccccgtt acgcagcagg cggccagcaa tgccagcacc ccataccgca
    19741 cccacggcac aggccgggag aatttcagga aacgcttcgg cgccagccag cggcggattc
    19801 tgaaaacaat atcctggaga atccccagcg ggcagatgaa ggaacagtat acgcggccga
    19861 acagcagcgt aagcagcagc aaaaccccca gaatcacggc ggaaacggct gccccgctca
    19921 gggtccccag cacggacggc acaaattgcc accgcgccag ggaaaaggaa tcttccatcc
    19981 cctctccgcc ccccgtcatg ttcggcacgt tccacgcggg aatgtggtgc agaaaagcgt
    20041 atgcaatccc aataaagaaa atgacggcca ccgtcacgcg gagccatttc agaggactga
    20101 cgagaaaagc tttgatgact gacatattta tcataaaccc gccggaggcg gaaaattggc
    20161 cccccaccgc gtcatccgca gacgggagaa ggagccgccg cgtcaggcca tgctgatacg
    20221 gcggatatta agcctggaaa ggtcggcgac gcccagcccc atttcctccc caagctgcac
    20281 atggcgaatt tttccggcct tgttcagcag ggcggcgccc gcagcgtcaa tggccacgat
    20341 gtcacgggaa gccagaaggg ttttgcgctc caccaggtca ttcgtatcct tccccacggg
    20401 gccattgcgc accatgggca tataggcgtc cagcacgcac aggtccggct tgcggaaata
    20461 cacggcgtcc gcaatgcact ggtgcaggtc gttcttatgc atggccccgc ggtcccagac
    20521 ggtacccatg acattcttca tgcaggcggt catctcggaa ccgctgtggt gcttgagggg
    20581 cgggcaggtg atataaacat ccgcttccag cacggcctta tgcaccttgg cggaggtgaa
    20641 tttccgggct ttcgggtttt ccgtccgcac gtactggctc tcattgccgc cgtccaccat
    20701 cacaccgcca gccttctcta cggcctcttt cacgccgctg ccctcataag cgccgttgtg
    20761 gcagcagtgg tcaaacaccc ttacttcctt cgccccggcc tgcctgcaca gctccaccag
    20821 cgcggcaacg gtttcaggat gagtgttggc gccgaattcc ggagccttgt tccagccgat
    20881 gttcggcttg attacgacgg tctggcccgg cttgacgaaa gcctgcatgc cgcccagcgt
    20941 ctccacggcc ttcctgacca tctccgcacg ggttccgttg cggacagcca ccagatccgg
    21001 gatgccggaa gccgccgggg ctccggcctc ctgggcgaac agggaatcca tgtccgatat
    21061 ggccgccagc gcccctgcgg cggcagtcct tttcaaaaaa gtgcgtctgt cagtcatctt
    21121 catggcaacg tctgtggttt atccccgcat gcgggaacac gcagaaggtg ttatgcctct
    21181 tcctaacaca cgccctgcca aaaaacaacc tgtcattctt cccgcaccgg aatttcataa
    21241 acgaattgtc atacaggcaa ggcttctgta caacccggtc atgaaatgtt ccctgctcag
    21301 ccgccccatg acctgcgctc atgactggat cagccgcgtc gtctcccccg gggacgcagt
    21361 cgtggacgct acggcgggca acgggcacga tacggttttc ctggcccggc tggttggccc
    21421 ttccggccag gtgcacgcct ttgacgtgca ggaagaagcc atccgcgcca ccagggagcg
    21481 tctggagaag gaagggctgc tcaccccttc cgtccggctc catcttgcca gccatgaccg
    21541 cctggcggaa ctggtcggag gccccgtgaa ggccatcgtc ttcaacctgg gctatctgcc
    21601 gggcggggac aaaaaaacgg tcaccaggac ggaatgcacc ctggcggcgc tggaacaggc
    21661 cgccgctctc attgccccga acgggcttct cagcgtcatg tgctatcccg gccacgaagg
    21721 aggaaaggaa gaagcggaag ccgtggaagc cttcctgtcc cgcctgcccc atcactcctg
    21781 gcgcgccggc aaataccagc tcctgaacac cggaacaccg gctcccttcc agatatgcgc
    21841 cttcaggctg gactgacctc ccccccgcca ggcgcccggg aaaaacctca ggcccggcat
    21901 tccttcatcc actccaccac ctgcgccaga gcgcgggcgc ggtgcgacat ggaatttttc
    21961 acttccatgg gaagctgggc gaaactccgg tcataccctt ccgggacaaa cagcggatca
    22021 tagccgaacc ctccttctcc ggccggctcc gtcaacatgt agccttccac ggaaccggaa
    22081 aactccgcca gcacgcggcc gttcccggca agcaccatga cgcagcggaa ccgcgcggaa
    22141 ggcctgtccc ccggcttcac gccggcgcgc cgcagttcct ccatcatgcg gaggttattc
    22201 cgcgcatggt tcccttcctc cccgccgaag gaggaggacc agactccggg gcgcccgccc
    22261 agcacatcca cctccagccc ggaatcatcc gccagcagga cgccggggat gcatctggaa
    22321 gcggaaagcg ccttgagcgt ggcgttctcc gtaaacgtga ctcccgtttc atccacgggc
    22381 ggagcctggg gatagtcgga aagatccttc acttcccatt tcccggccag catggcgcgg
    22441 atttccccgg tcttgtgggc gttgcgcgtg gcaaccacca gcagcggcaa ttcctgttcc
    22501 ataatcatca atcttttaca gttgaaaatc atctgaaaga ccgcgctcag cagtcaatct
    22561 ccaggctgac ggggcaatgg tcggaccccg tcacatccgg atggatggcg gcatccttca
    22621 ccctggaagc cagcggattg gaaacgcaga aatagtcaat gcgccatccg atattccggg
    22681 cgcgggcgcc cgcgcggtag gaccaccagc tgtaccagcc cggctcctcc ggatgcaggg
    22741 cgcggaacgt atccgtaaaa ccggcctcaa gcagcagcgt gaacccggcg cgctcctggt
    22801 cgctgaaccc ggcggaaaaa cggttctctt tgggacgtgc aatgtcaatc tcctcatggg
    22861 ccacgttcaa gtctccgcag aaaacgaccg gcttggtttc cgccagtccc gccacatact
    22921 cgcggaacgc ggcgtcccac tgctcccggt acggaaggcg caccagttca ttctgtgaat
    22981 tgggcgtata gcagttgacc aggtaaaacc cctcgaattc catcgtgcag acgcgccctt
    23041 cccggtcatg ctccggccag ccgattccga cggacgtgga aagaggccgc acccggctga
    23101 ggatcagcac gccggaatag ccggccttct ccgccggatt ccagagctgg tgcggccagg
    23161 aggaaatcca caggtctttc acctgttccg gacgcgcctt gatctcctga aggcagagga
    23221 cgtccggctc cagggcatcc acggcgtccg ccattccctt gcccagaacc gcccgcaatc
    23281 cgttcacgtt ccatgagacg agtttcatgc gcgcatactt cccgtttttg acgggtccgt
    23341 caagccctca aacaccgttg tcccgccccc tcccggaggg ctccggaaaa ttcttcttcc
    23401 ggaatcatcc ccagtctttt gcatgccgca tagaacaagc gttttttctt gccagaacct
    23461 ccccctcgtg cttacttaga taggcaaaca ccccgagagg cactcgagag tgggtctgtc
    23521 gacccgctct tttttgtcta tccatcacac tcctccacac tcatgacaaa cgatattaaa
    23581 gctttgattg actactacga gagggaaaaa gggctctccc gtgaaaaaat tctcctcgct
    23641 ctggagtccg cctttctctc cgcctaccgc aaaatggttc ccggttccgg cagcatcaac
    23701 tacctgaggg ccgaaatcaa cgtggacaag ggcaaggtgc gtatttttgc ggacctggaa
    23761 gtggtgccgg atgaagaata ttccgacaaa ttcaaccaga tccccctttc cctggccgtc
    23821 aagctggaca aaaacgccgt gcttcacgac ctgctcccca ccaacatcac gcccaagggc
    23881 ttcggccgca tcgcggtgca gaccgcccgc cagaccatgc tccagaaact gctggatgcg
    23941 gaaaaggaaa tgctttacga cgaattcaag gaccgcgccg gagacctggt aacgggcacc
    24001 atccgccgtt ttgaaaaggg ggatattttt gtggacctcg gcaaattcga aggcgtcatg
    24061 acctcccgcg aacgcgtgcc gaatgaagac tacagcgtcg gcgaccgcat gcgcttctac
    24121 gtggtggaag tgcgcacgga agcacgcggc ccggaagtca tcctttcccg cagccatccg
    24181 aacctggtgc gccgcctctt tgaatcggaa gtggtggaaa taggcgacca gaccgtggaa
    24241 atccacggca tcgcccgcga agccggctac cgcaccaaag tggccgtcat cagccatgac
    24301 gacaaagtag atccggtagg ggcatgcgta ggcatgcgcg gcgcccgcgt caaaaacatc
    24361 gtccgggagc tcaacaatga aaaagtggac atcctggaat ggacggaaga ccccgtcacc
    24421 ttcgtccggg aagctctcag ccccgtggaa ccgcgggaaa tcaccgtgga cgaggaagcc
    24481 agaaaaatct tcgttatcgt ccaggacgac aaagacctct ccaaggccat cggccgcagg
    24541 ggccagaatg cccgcctcac ctcccgcctg atgggctggg atgtccaggt gcgcgtcttt
    24601 gacgtccagg aagcggaaaa acgccagagc caggctgcgg ccgaagaagt catgcgccaa
    24661 tgccaggctg cggccaaaac cctcagcgaa caattggaaa tcccggaaga aaccgccatg
    24721 ggcctggtga ccatgggcgg aacggacctg gtggccctca cc
//
LOCUS       DAWEMX010000023        24555 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_830_length_24555_cov_11.922653, whole genome shotgun sequence.
ACCESSION   DAWEMX010000023 DAWEMX010000000
VERSION     DAWEMX010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 24555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24555
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_830_length_24555_cov_11.922653"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    364..373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7895..7904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18196..18205
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5090 a         6329 c         7337 g         5769 t
ORIGIN      
        1 tactaacgga cgggtggcgg gccggcaggg aatgggcgaa tgacataaga atagccggag
       61 cggggaaacg gcacggtcag aagaactccg gacgacaagg agacgcgcaa ggttgccccg
      121 tgtccggatg catgattgac aagccggaaa agcggggcat gctgttgata tgagatacga
      181 gccgcttcct tcttcctttt ttgccggcaa ccgtgaagag cttgcttccc gcctgcctgc
      241 cggcagtatg ctgattctgc atgccaacga cgtatttcct acgaatgcgg acggcacttt
      301 tgccctgcat cagaatgcca acctctttta tcttacggga gttgaccagg aagaaaccgt
      361 cctnnnnnnn nnncggctgg gatgagattc tgctgttgcg tgagacaaat gaacagattg
      421 ccatctggga aggcgcccgg ctctcgcagg aacaggcgag agagctgagc ggcatccagg
      481 acgtgcgctg gaccgatgaa tataatgcgc tgctggaggc cctggtgccg tccgcatcca
      541 tggtctttgt ggaagccaac cagcatccgc gatgcacatg cccggtggaa acgcgcaatg
      601 cccgcatgac caaggagctg aaggaaaaat tcccggacgc tgttttgaag aatgtctatg
      661 aaatcttggc ggacatgcgg caaatcaaaa agccggaaga aatcaaggct ctcaaaaaag
      721 cctgtgacat caccaatgaa ggcttccggg aattgctccg attcatcagg ccgggggtgg
      781 gcgaatggca gattgaggga ttcctggcca acgaattcat cagccgcggt ccgcgcaaat
      841 tctccttcct gcccatcatc gcttccggaa aggatacctg cgtgctgcat tatatccaaa
      901 acgacaaacg gtgcgaagac ggcgatctgg tgcttatgga cataggcacg gaatacggga
      961 attacaactc cgacatgacc cgcaccgttc ccgtgaacgg aaaattcacc ccccgccagc
     1021 gcgctgtgta tgaaagcgtg ctgaatatga tgacctacgc caaaaagatt ctgaaacccg
     1081 gaatcttgaa atcggagtac gaacgcctgg tgcgcgtttt tgccgccggg gaactcgtca
     1141 agctggggct gatcacaccc gcgcaggtgg cggaaaaacc gtccgatcct cccattgtcc
     1201 ggaaatatta catgcacggg tgttcccact tcctggggct ggatgtgcac gatgtaggcg
     1261 aagccaaccc cgttgtgttg ccgggcatgg ttttcaccgt ggagccgggc atctatattg
     1321 cggaagaagg cataggcatc cgtttggaaa acgacgtcct gatcggggaa acagaaaaca
     1381 tcgacctgct gggagacgtg cctttgctgc ctgatgacat tgaacggctc atggcccggt
     1441 aaaatgggcc ggatacggtt ctgaacttga caatggggcc cgtgtgggtt ttcattcccg
     1501 tcccatcatg tcgaagaaac tcaaaatagc tcttcccaaa ggcagtttgc aggattccac
     1561 cgtggaattg ttccgcaagg ccggctacaa tgtgtatgtg tccagccgcg gataccgccc
     1621 gacctgcgac gacgacgatc tggagctctt cctgatccgt gcccaggaaa taggccgtta
     1681 tgtcaatgac ggtttcatcg actgcggcat taccgggcgc gactggattt atgaaaacag
     1741 ggcggacgtg gaagtgctga cggatcttca atactccaag gctacctcca agcctacccg
     1801 ctgggtgctg gtagtcccgg aaaattcccc cattacctgt gcggaagacc ttcagggcaa
     1861 gcgcatcgcc acggaaggcg tgggcatcac ggaacgctgg cttcaggaaa agggtattga
     1921 agcccacgtg gaattctcct ggggagctac ggaagtgaag gtgccggaac tggtggacgc
     1981 catcgtggac atcacggaaa cgggaagctc catcaaggcc aacaagctcc gcatcgtgga
     2041 caccctgatg acctcctatc cccagtttat cgcgaaccgg gaaaccatgc aggacgagtg
     2101 gaaaaaacag aagctggaac gcctggttat gatgctcaag ggcgctctgg aagcgcgccg
     2161 gaaacgggga ctgaaaatga accttcctgc tgcttccctg aacggcctgg tagaggccct
     2221 tccgtctctg cgccgtccca ctatttccca tttggcggaa gaaggctggc tggccgtgga
     2281 aacggtcatt gacgaatccg ttgtgaggga tatcattccc cagctcaagg ccctgggagc
     2341 ggaaggcatc attgaatacc cgcttaacaa gctggtttac tagatttttt tctcaccaca
     2401 gtaaaacaac aaacgaacca caatcatggg atcgctcaaa aaacgccgta aagcaaaaat
     2461 caacaagcat aagcgcagca agcgcatgcg tcagaaccgt cacaagaagc gtttgcgtta
     2521 caagtcctaa ggtttgtgcg cttcgctgct caaactggca gtctgtccga tttccggaaa
     2581 attccggcgt cctccttatc gcggaaggac gccgtttttt atatggacac agcttttgca
     2641 tcattctcat ggattcattg ataaaacttt ttttattttt caccgcctca tgacaatgat
     2701 ttgtaaaaat tgtttcatga tttcctgaaa taaaaaagta gtcgatgaga gggagggact
     2761 ggaggaattt ggactggctg gaatcatatc aattttccgc atggaaaaag acggccatgt
     2821 ttttagtttt tgattacctg atataaactc gtgtatggat ttgtgcgtca cgggaatcga
     2881 acaggttata tctatggaga cggttgctta aaacaacgtc tgtcttccat ttctgttccc
     2941 ttttaaggaa aaccggaata atgctgatga gcggaatgga gcctgcgcat attccataac
     3001 gcaggggatg aaaaaacagg cggatgtctc gtgggccatc cctccgggtt gaaaggatgc
     3061 cttttttccg gttttaacgg caggccatgt cagactgttc cgcatcggcg ttttacgccg
     3121 ccttttcttc ccggaacctt ttcaggggaa tggtgatggt aaaaacagtt tcccggtggg
     3181 ggatgctgga gacggtgatg gctccgtcgt gggcttccac ggcgcgtttc acaatggaga
     3241 ggcccaggcc cgttcccctg atttccgagg agtggtgggt ttccacccgg taaaagcggt
     3301 tgaagaggaa ggggatgctt tccgcaggaa tgcccacgcc cgtatccgtg acggtgatga
     3361 cgcaggcgtc ctgtgttttg gcggcggcca cagtaatgga aagtcccggc tccgtgtttt
     3421 gcttgagggc gttttccacc aaattgaaca gaatttgcgt ccagtaaaag ggatctccat
     3481 aaagttccca atccgtggga atgtccatgt gcaggacggc ctgttttttc tcccggatgg
     3541 aatccagacg ggagaagacg tcgtccgcgc aggaggtgag gcggaaccac tgctccttca
     3601 gaatcgcctt gtggccggat tcgagtttgg agatcatgag catgtcttca ataatgttca
     3661 tcagcctctg gctgttctgg tgcatgactc cgatgaattt gcgggctagt ccgggacttt
     3721 cctccaccag atcgtcctcc atcaggtttt ccagatatcc cacgatgatg gtgaggggcg
     3781 tacgcagctc gtgggaggcg tttgccacga agtcccggcg catctggttg gtgaggtagt
     3841 tctgcgtgat gttgcgcagc aggatgcgct tttctctgat tggggcgtcc gtgatggcgc
     3901 tgtccagatg ccacgccgtg atgccgtttt gcacgccgcc gggggaattg gcggcgctca
     3961 gcgtgaattc cttggtgacg gggccgtccg tatcgaatgc ttcctgaaca tgccccagca
     4021 attcctggtt gcagaccagg gcccccactt tgcgcccttc cagcttttct tcctgaaaga
     4081 gttctttggc cagcgtatta gccagcacga tgtgtccgga tggaccgatg agcaggaagg
     4141 cctctcccag agcttccagg aagcgccgtt tgtccgcgat ggcctgttcc tgcgcttctt
     4201 ccccgcgctt cagcaggcgc tgagcgtccc gcaaggcttt tttccgggct ttcatggcgg
     4261 accgacatac ctggaccaga tgccagttca ggtacaggga ggccagaata aggatggtta
     4321 ggatgtagtc gatggccgac atggaaccgg agtgttaagc cttttcctcc gggatgcggc
     4381 gtcccggcag ggtaaagcgg tagccgatgc tgcggacggt ttcaatgcag tccgcatagg
     4441 gagccagttt ctggcgcagc cgcttcatgt gcgtgtccag cgtacggctc tgggcctggt
     4501 ggctgtaccc ccatacgcaa ctgagcaggt cgttgcggtt caggggctgg ccctcccgtt
     4561 caaccatatg gatcatgagc ttgaattccg tggccgtcag gtctacttcc tccttgtcga
     4621 tatagaattt gagggcgttt ttgtcaaaag tgaaggggcc gtattccgca atgacgctgc
     4681 ccggcgtgtg ctcgcaccgt ttcagcacgc tggccacgcg caggacaagt tccttggggc
     4741 tgaacggttt ggtcacatag tcgtccgctc ccagttccag cccctggatg cggtcttccg
     4801 cctgggcctt ggctgtcagg aagatgacgg gaatgttctg ggtgcggctg tcccggcgca
     4861 gttccctcag tacgccgtgt ccgtccatgc ccggcatcat ctggtccagc aggatcagtt
     4921 ccggcttgtc ccggcgcgcc acttccagcg cggtcagacc gtcataggcc gccaggggcg
     4981 tatggcccgc acgttccagg ttataggcca caaggtctga aatggcgtga tcgtcttcag
     5041 cgataagaat ggttgccatg tctgtttttt acgatgaaag aaggggcggt gtcccgatat
     5101 tagatgtgac gattcgttca catttccccg cccgggaggc ggttccggac agttaaagcc
     5161 tcctcttatg caaacatact gtaacggagg cgtttccgtt ttgcaaggct ggcccagatt
     5221 caacttctct tccctgcgtc atgtgataca cttgccccgc aacagttgta ttgttatgcg
     5281 aagcgataga gttaaagcag ggttcgagcg tgcgccgcac cgcagtttga tgcgtgccac
     5341 cggaatgacg gatgaggatt taggccgtcc tttcattgcc atttgcaatt cttttaatga
     5401 agtgattccg ggccatgtcc acctgaacag ggtggccgcc ctcatcaagg aggaggtacg
     5461 caaggccgga ggaactcccg tggaattcaa ccttcccggc gtttgtgacg gcattgccat
     5521 gggccacggc ggcatgaagt tttccctggc cagccgtgag ctgatcgcgg acagcgtaga
     5581 gacgatgctg agcgcccatg cgtttgatgc catgatctgc atccctaatt gcgacaagat
     5641 tgttcccggc atgattatgg gcgccctgcg ctgcaatatt cctactattt tctgcagcgg
     5701 cggtccgatg gccgccggca tggcggagga cggcacagtg ctggacctga acagcgtgtt
     5761 tgaggctgtc gcccgcttta aggcaggcaa gattaatgag gaggaacttc attccctgga
     5821 atgccgcgcc tgccccggcg ccggttcctg ctccggcatg tttacggcca attccatgaa
     5881 ttgcctgagc gaggtgatcg gcctggccct tcccggcaac ggttccctgc tggctacttc
     5941 agaggaacga aaggagttct ggaagcagac tgcacgccgc gccgtggaga tggcgaaggc
     6001 ggacggcccc ctgccgcgag acatcgtaac ccgtgacgct atcgacaatg ctttcacaat
     6061 tgatatggcc atgggcggca gttccaatac cgtgctccat acgctggcta tcgccaggga
     6121 agccggcgtg gagtatgacc tccagcgcat caatgatatt tccaggcgaa cccctaatat
     6181 ttgcaaggtg gctccttcct cccgcttcca catgcaggat gttttgcgtg caggcggggt
     6241 gagtgccatc attcatgaaa ttgccaggat tcccggagcc cttcatctgg acgccatgac
     6301 cgtcagcggg aaaacgctgg gtgaaacagt ggagggatgc ggcattgcgg atgaaaccgt
     6361 gattcatccg ttggaaaatg cctattcccg tgatggcggc ctggcgattc tgttcggcaa
     6421 tctggctgag gagggcgctg tggtgaaaaa ggcgggtgtg catccggata tgatgagttt
     6481 ccgcgggccc gccgtgattt tcgagtctca ggaagaggcc tgcgaaggca tcctggccgg
     6541 gaaggtaaag tccggcgatg tggttgtcat acgcaatgaa ggacccaagg gcggccccgg
     6601 catgcaggaa atgctggctc ccacttccta tattatgggg cagggccttg gcgcggaagt
     6661 ggcgcttatt acggacggcc gtttttccgg agccacgcac ggagcctgta ttggccatat
     6721 ttccccggaa gcggcggaag gcggcctgat cggcctgctg aggaacgggg atattattga
     6781 gtattccatt ccggaccgca cgctgaacgc ctgtttgagc ggggaggaga tcgcacgccg
     6841 ccgtgcggat tggaaaccta cctataacag ggtttcctcc tcctggctga gccgttaccg
     6901 ccagcttgcc acgaatgcca gcaagggggc agtcctccgg cgcggggaat aatagtttcc
     6961 cttttaacag cagccgcttc ccgtgatagc cgggaagcgg ttttttatgc ctgttttcct
     7021 tgctctgtag tctggcaggc tgcatggtcg gattgcccgc atccggcatt gaaaaaactg
     7081 cctgggtaaa gcggaaccgc ttcctgtgaa gggaagcggt tcctaatcaa caattcatgt
     7141 ttttccaaga aatgttttat ttggaaattc tgaggcggtc gctcattttt ttcagggaaa
     7201 gggcaattag ttcattttga gtggatggat agcgtttctt tatttccgat ggtgaaagtc
     7261 gttttcccgg agttggtgat ggaaagggag cgtcccttcc gtctcgtccc gtttgccgga
     7321 attatggata atagggtgga aacgggtttg agcggcgcgt acatttcggg aaaatagcct
     7381 cttcctgcgc agagagggaa atgattttgt aaaaaattcc gttgctgtcc gttatgctga
     7441 tgatgtaacc cgttttcagt attcgcagga gggcgcggtc ttgataagtc tggtttaccg
     7501 gaactgatgt tgcaggaagg ctgcggaatg acgcaggacg ggaccaggac atggcgttcc
     7561 ttgttgaagg aagcgttgga gatttccttc ccggttccct gcggaaccgg gaaggccgga
     7621 atggacatcc gttagaatga gtaactgacg gagccgttga ttccctgggc cgtcagtccg
     7681 gccctgcttt ccagagagta tccgatgcgg gccccccacc tgttgccgaa ggcggcgctt
     7741 atccctgcgt tgacgcgcag agcggtgcgg cctacgttga cgccgcgcgc cgtccaggcg
     7801 cccaagtcac tgctgacagt ccctttggga ttgaggcgac tgagatcctg cgtcacggca
     7861 actgtcacgc tgggcgtcag cgtggcattg gttcnnnnnn nnnngcgtag gtgcgggaga
     7921 gggtgatgcc tacaggcagc gtccagttct gggcgcgcgc tccgctgaag gtccgggcca
     7981 ggtctccgtg ttcggagaag ctgtccgttt tcccggtcat gaattccagc ccggcaaatg
     8041 gtgcgatgac cagtttgttg cggaattgtt ccgtccaggt ggcgtagatg gcggctccga
     8101 aagagtcaga gtcaaatttc ccggcagcgg aggtgtccgc caggcgggct ttcccgtcaa
     8161 aggatgtggt tccgaaggct gcatacccgt ccagagacaa tttggaggtt ccggcgggcg
     8221 tgtagcggcc gtagagcatg gccatgaaag aatccgcatc atagctggac agtcctccct
     8281 tgtcctggaa agttccgtaa gattggccga tggccactcc tgccaggacg gaggaggaga
     8341 gcttgcaatc cgcgcccacg gcatagccgc ctgcgtcgta tttgtagccg tcaatgccgc
     8401 tgcgcgaatg ttgatccgtc caggaaccca ggccgcccac ccatacggag ccgcggcctg
     8461 aagccaccgg cgccatggcg atgggggaac ccttggcgtc tgcagactgg acgggcttcg
     8521 ctgccgtaat attcagtccc tgtgcggcgg aaatggctcc ctgcgtcctg cccagggcgg
     8581 caaaaccctt gagagcatcg ttggaagccc aggccgcatt gatcacggtt tccccgctga
     8641 cggattgaat catggctgcc gccgcgtctg cgctggaggt tcccagaagg gtttcgaaga
     8701 acctgtgtgc ggctgaaccg tcccggacgc ccgtccggac ctgttccatg ccggctgctg
     8761 cgccggatat gttcccggaa tatccctgca tgggggcggc gactccgtgc atattttgtg
     8821 tcaggcggat agttccgttt tcatggggcg ttgcgcttgt ggagacgaaa gcggtgttga
     8881 acagaggcag ttcgcttact tcggctccgg tgatggtgac gttgtccacg gaagaagttc
     8941 cggcggcggt gtgtgtgagg tcgcggccgt tgcccgcttc atcgacattc cagagcttga
     9001 tggaggagtg gccgttgaac gcgatgtcat tgaggagcag ggaggaacct tcccccattt
     9061 caatcacggc gtttccgtta atttccaacg tgtctccagc cagtacggaa ttttccgtca
     9121 gaatgacgcc tgcgccattc atttcgtagt ccgtcaggtc ttcaactccc aattgagctc
     9181 cttccatgac catgatgtca tccacttcgg ccaggtggcc cacgatcagg gagccggctc
     9241 cggattgctg gatcaatgtt ccgccgaatc cgttatcctc cgtgtcccgg cctccgtaaa
     9301 gcaggtcgcc gccgcctgtc tgaatcagcg tggcggcgcc gcgcgtccac ggaccgtcgt
     9361 caagggagta atagtctgcg tcggaaatgc cgctgttgat gatggtgata tcgttcgcgt
     9421 ccgtgtccgc cagaatgacg gagccgtctt ccgcaaagat gtcgttagct tcttccccgg
     9481 ccgtgttgcc gtcaaagaag gatccggagc caatcagaag aatgcggttc tccatcaggt
     9541 ggatggcgcc tcccagcccg gaggcggcat tatccgtgaa ggtgacgtcc ctgccgagcc
     9601 agatattggc cccttcattt tcaaagaggt cctccacatt gtcgccatcc tgctgcgcga
     9661 ggttgcttgt cagttcggaa gcgatggctc cgccattggc tccagccgtg ttgccggaga
     9721 agacagttcc gtttcctacc gtgatatcca gcacttccgt ggtcagtttg gaggtgcgtt
     9781 ccccgtccgt ataggttatg gattcggcaa tattgttctg ggcgaagacg gctccgccgg
     9841 atcccgctgc cgtattgctg atgaagctgt tgttttctcc aataacgatg tctccatgtg
     9901 catagagggc gcctcccctg gagatatcca tggcagcgga cctgcccggg gccttgctgt
     9961 tcatgaatgt attgccgcgg cccgtggtga ttttgttttc gcgttccgtg acggtaactg
    10021 ttccgttgag aatattcccg ttttcatccg tgtcaatgat gctggcgcgg ataaggctgt
    10081 tgtccgcatc gctgaaggcg ctcatgtggt aaacgggagc atcttccgtt ccctgataag
    10141 ttacattgtt ccccagcgtg atggtatttt ccgcagctac ggtggtcatg gtcagcgtaa
    10201 cattgtctcc aatgactacg ttcccgccat aagttccttc cgcatattct ccggaagcca
    10261 gcacatagtc tccttccgtg ttttgcttga gcgtcccttc aaaggagacg gttccacggg
    10321 tggaagcgcc gtcgtctcca atggagccaa ccgtgtcccc tgcccagata tcggccatct
    10381 ggctgccgga aagatcgttg tcgcctgtaa tttcagtatt gcttccgata tagatattcc
    10441 ctccttccgc gtaaatatcc acatcgtcca gcgtagagtt tgctccaatg gagacggtgg
    10501 cctggtctcc cgctccggtg gtcatgcgca cggtgcggat tccgtcttct ccttcggtta
    10561 tggacaggga atccagttcc acggatacgg catccagtgt gacggatgtc agttttgccc
    10621 cttcgccgat gaagatggaa ccttgcgcat ccatgtcata gttttcaatg gaactgccgt
    10681 tgccgacggt gatgtctccg aacagcccca tggtttcctc catgctggtt ccgggaaggt
    10741 aggttccgtc ttcaccttgt ctggcaatgc cctgggaaag gacaagggct tctgtcgtga
    10801 tttccgccct ctccttggct gtcagcgtac tgttatctcc cagcagtatt tcacccccag
    10861 tggattggct gtcgccgccc acggtgctca ggttcgtatc ctgcatcgtc acggctcccg
    10921 cgctgaagtc gatccctccg tgagcgccga cggtttccga ctgaccgatc aggatgccgg
    10981 aatcttcatt ttcataaacg gatgtcgtat tgacggcgcc tgtagagatg agcgtggaat
    11041 cagataagga aatatctcca tgggagacaa tttggccgcg gactccatca ttcatggata
    11101 aatccatttc atggatacgg acattttttc ccagatccat ggcaatttct cccggtgtag
    11161 aggtgactac cgtggagatg agggctcctg tttcgggatc aattctggtt tctatggagg
    11221 cggaatgccc ggtagtgacc tcattccggc tgagaacggc tccgtctccg gccaccagct
    11281 tgttgttttc cagcacaagc caggttcctt ccagcgtgga agcgttttct atggaaaggg
    11341 tgtttccttc ttcgtcccat ccgctgagca tgccttccgt catgttgccc acgtgaacgg
    11401 ttccacggcc gctcacgtta agatgctgca tgttcacggc agacatgtcc ttgtagttga
    11461 agttggagat gcttcctttc ccgtctgatt tgaggacaat ggaggcgtct gcgggcaggg
    11521 agcccaattc tactccttca aagtcgatgg agccggtaat ggtcaacgtg tctccatcag
    11581 ccatctggtt ccagactgtt tgaaattctt ccagggtgga agctgtatat tcggcggcga
    11641 aggtttggct tccaaggacg cacatggctg ccagaagggc ccggcgcaac gggaggggta
    11701 aacgaaggtt catggtttgt tatattcaga ttaattcagg gaaaagtgga gatgaatgac
    11761 ttttccctgc tgtttatgta caggattgtt aatcctgtgt ttctgaatat atcccattcg
    11821 ttttttccgc tttgggcaca taaatgctca tggtgcctcg gaagattgaa tcaacggttt
    11881 atctaacaaa tagaattgtg gttgtacata aagaattgtc cgtaaaaaga gattaatgga
    11941 taaaaatatt gtaatttttt cttgataaca ggattaacaa tcctgtgatg tatcaggtat
    12001 taatataaaa aacatgatat tcggaatggc gagacatgga aaaaaccaga aggaatatct
    12061 ggctgcggaa atgttattga aaggtagcgg attgaatatg ctggatgctg ccagcctcgc
    12121 agtagagctg ctgagcctgt gcggaggcag ccgcagcatg cgccgggcca gaaaggcaat
    12181 ttttctgggt gctgaggaat tgaggaaagg ggaaaggacg gtttcctttt ccgccgccgt
    12241 ggaagaaatg ctgaaggcga agcgtaatct tcgccctacg acgttgcgcg atatcaggta
    12301 tttcaccgga gctttgatgc ggaggtgtcc ggaattgaaa ggttttccgg tccggaagct
    12361 gacgccggag cattgcgccc attttctgga ggctgctttt acatcccaca ggcagcgtta
    12421 caagggccgg gcggtgatga gcggcgtttt gtccttttcc ctgcggcgcg gatggtgcgg
    12481 ggaaaatcct gtcgcccgtg tggactctcc ttcgtttcgt gaacggacga ttgccatttt
    12541 ggctccggaa gagataggaa gccttcggac ggtggtggaa gctccggaat ttcgtgactg
    12601 cgctccggct gtttgggtaa tgttgtacgc cggaatcagg ccgggggagg tggtgcgcct
    12661 ccattggcgt gatgtggatt tggaggagag ggtcatttcc gtaaggtccc gaaccagtaa
    12721 aacgggggga atcaggcatg tcaccattca tgctgtgctc tggcggcttc tggcgggata
    12781 cggagcaggg ggggcagccg gactgttatg tcctcccaat tggccggtgc gctggaggct
    12841 gctccggaaa aaagccggat gggggctgca caaccgcttt ggagaatgga gtgcggatgc
    12901 gctgaggcat acttatgctt cctatcacgc caaatggttc agggattttt ccctgcttca
    12961 gctggagatg gggcatcgtt cctcctccct gttgcgggag cggtacctga acatggaagg
    13021 ggtgagccgc gacagggcac gccttttctg ggaagcgccg gaacatggct ggaacaacaa
    13081 aaccggcatt gcaggaacgg actttctatg aagggacggc tgtttgactg ccgcagccgg
    13141 aaaagggcgg catgagggag tccccatggt tatgccctgg gcaggaagta gccgcgctgt
    13201 ttttctgaga tgggcatgtc caggcgttcc ataacggcca gcatgtcttc ccataccttg
    13261 cgtttttccc gcgggctgtc cgatctgagc aggtaactgg gatggtaagt gacgcgtacg
    13321 ggaatgccgt taaggtcgtg gaacctgccg cgcatggaac tgacggatcc ctgttccccc
    13381 aacaggccta cggcggcggt accgcccagg gctacaatca ttttcggctg gatgacccgg
    13441 atttcatgca tgatgatggg cagacatgct tcaatttctt ctggcgtggg tgcacggtta
    13501 ttggtccgct ggttggggag ggccgggcgg aatttgacga tattggaaat atagaccatg
    13561 tctctggaga gccccatggc ccttaatatc tggttgagtt tttccccggc acggcccaca
    13621 aagggctcct gctgcacttc ttcataatat ccgggagctt ccccgatgag catgagtttc
    13681 gtatggggat tgcccgtggc aaagaccatg gtgtcccgca gggagttgag ttcccgcgcc
    13741 ggtttccagt tctgcgccag attacggagg tattgcagct tgtcttccat tgtcccttcc
    13801 gggagggtta tggcggagtc cggttccctg ttttgcggaa acagctccgg cggttccggc
    13861 tttccggcag gagcggagac aggagcggct gcctgcaggg gcggcggttc cggagaagcg
    13921 ggtgctgatg cggcgggacg gcctccctgt ctggaagcca cgtaccatgt tctgagaacg
    13981 tttctggctt catccgtcag gctcgttcgc gttgttccct gctggatcag ggaatcaaga
    14041 taatggtgga aaaggctgtt cggtgtagcg ctcatgggag gataaagcca gattagtcca
    14101 taagaggccg tctgtcacga cgattcgcag gcttcccggc cttcatggaa cgcgcctctc
    14161 cgggaaatga cacggtaaaa gatggtatct tttgcaaggg aggtagcaaa ttggggcttt
    14221 ccggttcagg aatggaaatg cagccggctc tctcaaaatt ttacttagtt cacgtatgtc
    14281 tttttctgaa gaattaaacg atttgatgga ttgccccact cccctggtgc gccgtttcat
    14341 ggcgttgctg gaaccggttg acgacgcccg cctggaggag atggcgcagg agagccggcg
    14401 tctgacgcgg ctgcatttcg gccggaccat ccgcctgttc gcccccattt acctgtccaa
    14461 cgaatgcatc aataattgca agtactgcgg tttttcccgg gataatccca ttatccgcac
    14521 cacgcttacg gtggatgaag tggtgcagga ggcccgctac ctgcacggcc tggggctgcg
    14581 cagcatcctg ctggtggccg gggagcatcc caagttcgtt tccgacgggt atatgcagga
    14641 atgcctggac gccctgcatt cctttattcc atccctgggg ctggagatag ggccgttgcc
    14701 ggacgaccgt tatgcggaga tcgtccgtca cggggcggaa caattggccg tgtatcagga
    14761 aacctataac cgggaagtgt atgaaaccct gcatacggca gggatgaaga aaaatttcaa
    14821 ctggaggctg gactgcccgg aacgcgccta ccagggcggt ttccgccgca ttcagatagg
    14881 agccttgttc gggctttcgc cgtggcggcg ggaggccatg tcgcttgccg tccatctgga
    14941 ttacctgcag aagcattgct ggaaatccgc gctttccgtg gcgtttccgc gcatgcgtcc
    15001 ctacgccggg aattacgagt atgaacctga tccggacttg atgctggatg accgccattt
    15061 tgtccagctt atggccgccc tgcgcatctg tttccccaag ataggcatgt ccatcagcac
    15121 ccgtgagccc gcgccgatga ggaatgccct gatgcatttg ggcatgaccc acatgtccgc
    15181 cattgcgcgc acggaaccgg ggggctacac gggcgtggga acggctgccg cccatttgac
    15241 ggtgcggggc aaccgggtgg atcttcccga tggccggaaa gggaattgca aggcgacgga
    15301 gcagtttgag atttccgacc agcgcacacc ggagcaggtg gtcggagcca tacggaatgc
    15361 cgggctggag cctgtctgga aagactggga tgccgctctg gatgtggtgt aggagaacct
    15421 tctcccgtct catttctcca gaatgacatc catatcgccg tgatgttgtg atggagcatt
    15481 gacggcatgc tccggctgtc atatttccgg tagcgatgat tcgttattat tgggggaggt
    15541 tttggccgtt cctcctgttt gcgttcggca ttgaggcggt ggaaaatctg tttacggtat
    15601 ttttcgaata ccgcaacatg gatttcgggt tgcttcccct cctgaaaacg gcgtacgttt
    15661 ttgtgacgga gtttgccgtc accatgtgct actggcttat tccctatgcc gtttatttgt
    15721 ggattctgcc gcgcgggaaa gcaggaggga aggcggacag gtggctcacc tccgcatggt
    15781 ttttcctgtt tgtgctcgcc aatctgtttg aagatgtggc ggaagctttt ttctggaacg
    15841 agtttgaagc cagcttcaat ttcattgcgg tggattacct ggtttacacc aaggaggtta
    15901 tcgggaatat ttacgagtcc tatcccatca ttcctattct gggcggcatt ctggcggcgt
    15961 ccgttctggc cgtctgggga atgaagaggt tcctgcttcc caggaacggg gcggttcccg
    16021 tcggatggaa acgaggctgt gtggtgctgt tcctgctggc ctgcgtcacg gggggatatt
    16081 ggctggtgga tatcaaggat gcggatgccg tgaacaaccg ttataattcg gaaatggcca
    16141 aggatggcct ttacagcctg ttcagcgcct ttctcaagaa tgaactggat taccgcgctt
    16201 attaccggac gctgccggat gcggaagcgg cggcgtttct ggcccgggag ttcacggcgg
    16261 atgacacgtc cgtgccggag gcttcgtccg gcagcgtaaa gaggcaggtg cgtccttccg
    16321 ggggggctat ccgcccgaat gtcgtggttg tggtcatgga gagcatggga gcggaatttt
    16381 tgaacgagtg ccgggaagac ggggctgacg tcactccgtg cctgagccgt ctgggaaagg
    16441 aaggcatttt tttcccgaat acttatgcca caggcacccg ttccgtacgc gggctggaag
    16501 caatcagcac atccctgccg ccgcttcccg gcatgtccat ccttcgccag gaaggaaatg
    16561 agcatttgca gaccataggt tccatattca gggacaaggg atatgatctc aaatggattt
    16621 acggcggcta cgggtatttt gacaacatga attatttttt cgggaacaac gggtttcagg
    16681 ttctggaccg taattccatg gatgattccg aggtgaccca ttccactatt tggggcgttt
    16741 gcgatgaaga tttgttccgc cgcgccgtac gggaggcgga tgaatcctgc ggacgcggca
    16801 agccgttttt gcaggtggtg tttaccacgt ccaaccaccg cccctacacg tatccggaag
    16861 ggcgcattga cattccttcc cacacggggc gcatgggggc cgtgaaatac gcggattatg
    16921 cggtaggcgc ctttgtggag gaggccagaa ccaaaccctg gtttgacaac acgctgttcg
    16981 tgttcgtagg agaccacggc gccgggagcg cgggaaagca ggccctcaat ccggaaacgc
    17041 accgcatttt ttccattttc tacgctccgg ctctgctgaa gccggaacgg cgggacactc
    17101 ccgtgagcca gattgacgtg ctgcccaccc tgctggggct gttgaactgg ccgtatgatg
    17161 cggcctttta tggaaaggat gccttgaagc cttcctatca gtcccggtat tttgtgagca
    17221 attaccaata tatcggctat ttaaagggga aagacatggt agtgctcaaa ccccagcgcg
    17281 gagtggaatt tttccgggac ggagaggccg ttgagccgga tgggcggatg aaagagctgg
    17341 aaagggaagc ggtttattac tatcagcacg cttccggctg gcgcaccagt ttgaaggaat
    17401 aaggcccccg caaggtgcgg aggccgtgat tttggcggcc tccgcccgcg cacggaaaat
    17461 gcgttcagga tttgcttccg tccctgtaat cctggagagc gtcccaatgg aacgtgaagt
    17521 gcttctgcat gtgcagcccg ttggcgagaa tgaacatgag ggaaatgcgg aattcgttat
    17581 tgttcaatcc ttccagctct tttttgtcaa ttttcatgga gaatttcccg ctcttttttc
    17641 caaggcgccg ggagaaggaa acggcatcat agtcctggtt gaccgcataa ggaggatcct
    17701 ggatgtaaac attcagagct ttgacggtct gggggctttt ataattgccg gaaaccagaa
    17761 tctggtctcc tttaaaagaa atggctacgt ccccgacctc cacttccggc ttgccttcgt
    17821 agaattgctg ggccggggtg acggaaaaca cttcacaggc atccagcagg gcgcagctgg
    17881 ccggggtcag gaacgtggga ctggtcccga acgtgtaatt gcccgaaccc atcagggccg
    17941 tgccgtattt tttaccgtcc gaggcggtct gatggttgtg cggcagatta aggccgtgcc
    18001 ccaattcgtg ggccatgcct ccataccatt tggtcagcag ccttccttcc cttgttttct
    18061 gccccaggtg tttgatgtcg aaggccggat aatccagggc gaaacagttg cgccccatgc
    18121 cgtaaaaggg aactccgccg ggattgtcgg ggccgttctt ttcgtcattc caggtgggca
    18181 tgatgataag cgtgtnnnnn nnnnngtgtg ctggcttttt ttcctgtccg gatgggcttt
    18241 gaaaaattcg tcaagttcct gggccgcttt ccagccgcct ccgttttcat aaggataatg
    18301 ggccgccgga tttttggcct tgtattcaat gatgttcacg cggcctgggg atttgatatc
    18361 cagaccgaag gaacgcgcgc catagccatg ccgttgcatt tccttgccgt aaaactgctg
    18421 gaggtaaagg agcagttcgc tgaggcgccg ttcataatcc ggaacgggtt ccgtatcact
    18481 gcccagaaaa taaacgatat tgagctgacg ggccgttttt ttcagggcaa tccggtcttt
    18541 caggacgtcc ggaacggtga cttccgcaaa ggatgtaccg gaggctgctc ccagaaagaa
    18601 cagagaaaaa aatgcggaga gaagtcgttt caacatacat gccaaattag aagcgtcatt
    18661 tttaaagaca aggaaattta ctgttctctc cgccattgga ggaagcaccc ctgggccggg
    18721 agggaaataa cggggggaag aatccgtcat gtggcggccg ataagttcgg aatggctgaa
    18781 agatagaggt tgcgaaggca gtaaatatgt gacattgttt attaaagcat gcatttccaa
    18841 tctctatatt tatctgccgt ggcggtaagt gccgtttctt tcgggtgggg ggctctggat
    18901 aagccttccg cctccaattt gatctggtcc gaccaacctg cggtcattgt ctatccccaa
    18961 gaggacaaag ggtgcggtcc taatggtagc tttggggttt ataaaaaacc gtcagctgtt
    19021 tgggaagcgg aggggtatcc cattggcaat ggtcgcgttg gagccatgat tttcagcgct
    19081 cccggccgcg agcggctggc cctgaatgaa atcagccttt ggtctggagg agccaatccg
    19141 gggggagggt acggttacgg gcctgatgcc gggacgaacc agttcggcaa ctatctcccc
    19201 tttggggatt tgtttgtgga ctttaaaaaa ggcgaccagc cggcttccct gtctgtggaa
    19261 gattttacgc gctccctgga tctccgggac ggcattcata aagtgaatta caaggcggat
    19321 ggcgtaacgt atgaccggga ggcattctcc agcacgcctg ccaacgtcct ggtgctgaac
    19381 tataaagcca gcaaacccgg ccaattcagc gcggattttt ccgttaacag ccagcttgga
    19441 gcagatattt ccgccaaggg atccgtcatc acctggaagg ggattttgaa aaacggcatg
    19501 aattatgaag gccgcgtttt gatccgtccc aaaggcggta cgctttccgc ctcgggagat
    19561 aaaatttccg tgaaaaatgc ggattcctgc atggtcgtca tcgccatgga gacggattac
    19621 ctgatggatt ataaaaagga ctggaagggt gaatctcccg ccaggaagct ggaccgttat
    19681 gcggccaaag ccgcttctgc ggattatgcc gccctgaaac aggcccacat ttcccagtac
    19741 aagtccatgt ttgaccgggt gaaggtcaac ttcggaaaaa cggaggagga tgtagccaag
    19801 ctgcctacgc cgaagcgtct ggaagcctat aagaaaaatc cggcagaccc cgatctggag
    19861 gaaaccatgt tccagttcgg acgctatctg ctgttgtcca gttcccggcc cggcacgctc
    19921 ccggccaatc tgcaagggtt gtggaatgat tatgtcaaac cgccgtgggc ctgcgactac
    19981 cataacaaca tcaacgtcca gatggcgtat tggggggcgg aacccgccaa tctttccgaa
    20041 tgccatgagg ccctggtcaa ttatgtggag gcaatggccc ccggctgccg ggacgcttcc
    20101 caggcgaaca aggggttcaa taccaaggac ggcaaacccg tgcgcggatg gacggtgcgc
    20161 acctcccaga atatcttcgg tggcaacggc tggcagtgga acattcccgg tgcggcctgg
    20221 tatgcgctgc atatatggga acattatgca tttaccggtg atagaaagta tctggaaaaa
    20281 caggcgtatc ccctgatgaa ggaaatctgt catttctggg aagaccattt gaaggaactg
    20341 ggagccgggg gcgaagggtt taagacaaac ggcaaggatc cgagcgaaga ggagaagaag
    20401 gatctggccg atgtgaaagc cggaactctg gtggctccca atggctggtc tcccgaacat
    20461 gggccgcgtg aagacggtgt gatgcatgac cagcagatta ttgcagagct tttctccaat
    20521 accatcaaag ccgcccgcat tctgggcaag gacgccgcct gggccaagag cctggagggc
    20581 aagctgaaga ggctggccgg caacaaggta ggcaaggaag ggaatcttca ggaatggatg
    20641 attgaccgca ttcccaagac ggaccaccgc catacgtccc acctttttgc cgttttcccc
    20701 ggcaaccaga tcagcaagct caagacgccc aagctggcgg aagccgcccg cctttccctg
    20761 gaatggcgcg gcacgaccgg agacagccgc cgttcctgga cgtggccgtg gcgcacggct
    20821 ctgtgggccc gcctgggcga ggggaacaag gctcatgaga tggttcaggg gctcttgaaa
    20881 ttcaacactt tgccgaatat gctgactact catcccccta tgcagatgga cggcaacttc
    20941 ggcattgtag gcggcatttg tgaaatgttg gtgcaatccc atgccggggg cctggacatt
    21001 atgccctctc ccgtggaagc gtggcctgaa ggctccgtga aggggctgaa agcccggggc
    21061 aacgtcacgg tggatttttc ctggaaggac ggcaaggtaa gcagcgtgaa gttgtattcc
    21121 gcccaaccca aggtgttgcc cgtgcgcgtt aacgggaaga tgacccgcat gaaaaccctg
    21181 ccgctgaaat cgggagcgga atcttcgcag cctgcggccc ggtaaaaagg gaaatccatg
    21241 atccggtttt aattccggaa ctaaagataa tcatggcttc cagtcctttc gttagggctg
    21301 ggagcctttt ttatcccgga cctttgcggg agggacggtg tttgtttcaa aggaggagct
    21361 tgctgaatgg cgcgttccga aagaaggaat tcctctcggg atgtaaagat tgggaagggt
    21421 tttcgcaagg ggcggtcctt tgccccgtct taaggaaaga agggctgcgc tctcatttct
    21481 tttccatctg cctgagcagg gagcaaatgg cgcaggaatg gagggaacac aggtcttcgt
    21541 gaatttgaag caatgcctgg tggtgccagg ctttcgcaag aaaggcgtgt gcatccggcc
    21601 tgccgcccag cagggattgg tggacggaga ggacttttgt tcccgcctgt ccggcccgga
    21661 gggaaagata atgcttccat gcatgttcgt catctttcat cggaagaagg tggttgatga
    21721 tgaagtccag aattctatcc cggcccatca gggccatggg gcggggagac ggtggggaag
    21781 gaagcgttac atgggtgctc cagtaggggt gtgtaaggga ggagaggtac ttcgtcagct
    21841 cttccgtatg tccggcaagg caaaggcgct taaacgcatc aaaatctgct gcgatgacgg
    21901 ccagagcccc tacgcgcctc tgcggatggt tggccggcct gttgccggaa tacgtccagg
    21961 gaaaggagcg gtccggcgcc agttcaaatt gtgcccgcag gggccaccat tgagcccaga
    22021 gttttttgtg gagttctacg gcttccggtg tgcaggaggc cggaagaacg ggaaggagga
    22081 agcccgccgt accgaaaagc agggcttccg gacagttccg gatggaacgc aggggcgccc
    22141 gcagggccag gtggcgcatg gcggttttgt tagcgtgata gcccagcgtt tccgccaggt
    22201 tttcaaaaag ggcctgttcc tctcctgcgt atttggcgcg ttccgcatgg cggcggtgct
    22261 tgttccggaa cctgtaggct gccgctgatt ggaggagtgt ttccagaaac tccggagcca
    22321 tggatgccag cagtccggag tggcggcagt ggcgtaccgg ggcgctgccg gaaggggatg
    22381 ttcctgaccg cgccagcgca gcggggggaa tgacagccag aggaacgcgt tcatgcttgg
    22441 cattgcgggt gaaccatttc ctgcggctgg gcgcgcaggc gaggtgcagg atgacgccgt
    22501 taaaccccgg gttggaaccg tgcccgtggc gttcccagtc ttccggcgcg ggatccagtt
    22561 cgatatctcc cgattgcggg acgccattaa tgcatatcct ggcgtttaga aagtccggtc
    22621 cggcggattt attccagttc ccgaaatcca gaatgtgaac gtccctgcct gaatcatccg
    22681 taaaagaggt tccaaatact ccttccagca gaagaagctg aagggtccgc tcgtccggaa
    22741 gggcggaatg attggaaacg gaatgttccc tgacggcggc cggcccttct tcccttgctg
    22801 cggaaagggc ttcctcatac agggaggcca tggagcgcag gtccatgtgg aggggaggca
    22861 attttattga tctttctgcc gttccaattc ttctttcagg cagtcggtta cgttgcggaa
    22921 tttttcttcg ttctcttcgc tcatttcgct cagggtcaaa tgggagttga gaacgtggag
    22981 agtttgctcc ttggcatcca agtgaacgga aggggcgttt tcagtaccca gggcggcacg
    23041 tatctgctct acttttcccc tccagggggc tgtgggcggt tcaatgctga gcagggcctc
    23101 cagccccagg ctttccaggg cgctcaggca gcgttcgctc ggggaagcga tttggacgga
    23161 tcccccgatg ggaagcagcc gtcgcgacag gcccgcaaga gtacccatga aggtggagtc
    23221 aatgccggag caggtttcca gatcaatgac gatcagacgc cctccggaag cgatgtaatc
    23281 gtcccccagg gatttgacag tcggactatt ggcaaaactt ccgcgtccgg aacaccggac
    23341 ccaaagaaat tcatcaaaaa tgccgaaaag gatggaagaa ttgttattca cgtcgttaga
    23401 aaatggaaac catatgaata acaagagaaa cctctgcggt caagtcagga tttaatttgc
    23461 gcgccgccgg aagagggttc gttttctcgc atgcagaacc ttttcccgcg gcgctctttt
    23521 cgttattcgg agggatgcct caggagttct tggtgccttc ccccgtaccg ggcaggaaac
    23581 ggtaataggt ttccagcatc agcgttgcca ggcactgacg gtagatgctt tcagatttgt
    23641 tgctcatgcc ggaaacgtat cctacgtcat ttttccggaa actgccgtcc gcattctggc
    23701 cgctgagcac ggcgtcccgg aacgctttgt tgtaggaggt ccatacgtct cctccgcggt
    23761 tcatggcagc ctgcacgcag tagtaatgca tgtagagatt gctcttgacg ttttccgtgc
    23821 cgcttccctg gccccactgg agagttttga cgttgttttt gatccagtcc agacccttgc
    23881 gggcggcgct gtccgagccg ttgcccgtca tttgcagaga aagcacgccc acgcctacca
    23941 gggaagaacg gggcatattg ccgttttcct tataggaaaa tttgccgtcc ggcatgacgt
    24001 tcttgcgcac gtagcttgag gccttgcgca gcgtagagcg gatttcccct ttggtaggcc
    24061 tgacgccgcc gtgttcggca gccttgagag cctgcacgtt ccatccggtg acggaaaggt
    24121 cgatgtccgg attaatcttg ttgttgtagc tgtagcccca gcccccgttg gggcactggg
    24181 cgtcaataat cctgtccacg gcttttacga cgaccttttc cagattggag atggagggga
    24241 tgtccatctg tttacagaag gtgtaggctt cgcacagggc ataggtggct accgcgtggt
    24301 cataacagat ttccttcagg gcgggctgtt ccgaaaggac gggatttttc atgcctaagt
    24361 ccacgaggaa ggaaatgccc ttcaggacgt tttctccgaa ttcctcggaa agaggggttt
    24421 cgcagtgggc cagataggcc agcagggaaa tgccggtcat ggaagcgcgg aattgtcctt
    24481 caccccagga gccgttgccg ttctgctgtt ttttcagcca gcggagggat ttgactacgg
    24541 cttcctcaca ctgcg
//
LOCUS       DAWEMX010000024        24225 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_854_length_24225_cov_12.535043, whole genome shotgun sequence.
ACCESSION   DAWEMX010000024 DAWEMX010000000
VERSION     DAWEMX010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 24225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24225
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_854_length_24225_cov_12.535043"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2267..2326
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5839..5848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14388..14397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23487..23496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4738 a         6208 c         7586 g         5603 t
ORIGIN      
        1 gtttggggtc cgtctgggcc gggatgccgg aatccgtgat gcggtaaatc aggttggtgg
       61 tttggtcccc ggaaatgccg aaattgcagg gatgataggg ggagaacgcc ttttcccata
      121 ttggttttcc ttcccttttc cagtagtccg taatggaatc tcccaggaaa aggacgccgc
      181 aggggttgtt ttttatttgt tccacctgct gttcatattg cttcctccac cattggggct
      241 gttcccgtgg cagaggagtc agaggggacg gcgcagccgt tgagagaagg gaggccagca
      301 gggagaaagc aagtatgtgc aaaatcttca tgggcattta ttatacgggc attttccaaa
      361 aacgccacat gaaagtggtg gccttttttt gctgctgcgg cgtgcgggca gcttttgcgg
      421 cagggtgttc ccccttgcca gattccgggg ggtggtgtag cttgtgggcg gtatgagtga
      481 ggtatccctg cagcatggaa tgacgcgcca gatggccgtt tcccagtcca tgcagacggg
      541 gatgcagatt cttcaggctt ccgctttgga attaaggcag attatccgcc aggcgctgga
      601 aaccaatccc atgcttgagg agttgccgga cgcttccccg gaagcgctgg aagacggtcc
      661 ggacggggat gcctggaatg agcgggagga cgggtggaac gagtttacgg cggaaggccg
      721 cctgtctggg gacgcggccg cccgccgtga tttcatgtat gaatccgtcg tggctccgga
      781 atccctgaaa acccatttga tggaccaggc gcagcattcc gcattgacgg gccgcgcccg
      841 ggacgccctg ttcctgctga ttgacgcttt ggacgaacgc gggtttctga cggagtctcc
      901 gcaggagctg gaagagcagg ggtgtttcag catgcgggac atggaagaag ctctggctgc
      961 gctgagggaa atggacccgc ccggagtggg ggcggcggat ttaagggatt ccctgctgat
     1021 ccagcttgaa cagcgcggtt tgaagcgttc gcttgctttc cgcctggtca agcgctgctg
     1081 gcgggaactg gcggcgcaca agtatgagga agccgcacgc ctgctggacg tggaaccggg
     1141 ggccgtggcg gcggctctgg aggtgattag gagcctgact cctgatccag ggggggccta
     1201 cgcccccggc ggaaaccctc atctgctgcc tgacgtcatt gtggaggagg ggccttccgg
     1261 cgtgctggag gttatcctca cttccgagta tcttccgcgc ctgtccatga atgaacggta
     1321 tatggagtta atggctgagg gttccggaag ccgggagctg aggcaatatc tccgcagggc
     1381 gttccgtgag ggacaggagc tgttgcgggc tttggatatg aggcaggaga ccgttttgcg
     1441 cctggcccgt gtcattgtgc ggaggcagga ggattttttc aggtccggcc cgtcccgcct
     1501 gaaggccatg ggcatggaag aagtggcgga ggagatgggg gttcatgttt ccacggtttc
     1561 ccgggcgtgc cgggacaagt acctgttgtg caggtggggg atgaaagaac tgagatcctt
     1621 tttcagtgcg ggagttccgt ctgaaggcgg cgtttccccg gacggggcgg cttccggagc
     1681 cgtggctgcc ggcgccgtcc aggagctgat gcggcggctg attgcggagg aagattcttc
     1741 caaacccctc agtgacgccg ggctggcggc cgcgctgcgg gaaaaagggg tgaacgtcgc
     1801 ccgcaggacg gtggccaaat atcgggagca gatgaagata ctgcccgctt ccctgcgccg
     1861 gggaatatga cagtcctcta tattttgaat tgtttattaa taatttaaca atcaatgtat
     1921 tgtgtctttt gttaatggga cgcttttttt gtttttcttt ccgacggaag aggcggttga
     1981 ctgatgcggc tttggtgcta tagtccccgc tttatcgtga tgcgtacatt tacctggcct
     2041 attgccctgc tgctggcggg gcttcttttc cagacagtgg cgtttgccgt gttgaatacg
     2101 gttgttcccc tgtggatgga gcaatttgat gccgccacct gggaggcagg gctggtggga
     2161 gctttctttt ttctcgggaa cctggccgga acgctgctgg ctgacggcgt gattcgccgg
     2221 gccgggttca aaggaagtta ccaatatgca tgccttttat gcgcagnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgg cctggagcgg
     2341 cctccggctg ctggcgggga tcagctgcgc cctggtctgg gtggtggtgg aaagcgccct
     2401 gctgagggcc ggaacgctgc aaacccgcgg cattctgctg gcttcctaca tggtggttta
     2461 ttatctgggt acggtgctgg ggcagttgct tctgggctgg ttccccagcg atatgcccct
     2521 gattgtgacg gaagtctgca ttttatcagt agcgggcatg gttccgctga tgttcgcgcg
     2581 tctggggccg ggcaatggac aggtttcatc ctcctcccat atagaaattc ggacactgct
     2641 gagacgccgc agcgtctttc tgggtgttgt gggatgtgtg atttccggcg tggtattggg
     2701 tactatttat tgcctgatgc ccctgttcct gaagcaccag ggaatggacc actcttccgt
     2761 gggatactgg atggccctgc tgattgccgc tgccattctg gggcagtggc ccatggggcg
     2821 gctggcggac aggtacggcc gcgctttcgt catgaaatgc cagtccctgc tggtggcggc
     2881 ggcctgtgcc gggctgatgc tgaagggggg gctgatggct ccctccctga ttgctctggg
     2941 gctggccgga ttttccctgt accctgttgc catggcctgg ggatgcgagg aagcttcccg
     3001 ggatgaactg gtgaccatga accagcttct gctgttgagt tattccctgg gcacgctggc
     3061 cggcccttcc ctgacttcgt tcctgatgca gaggtattcc gacaattgga tgcctatggt
     3121 tattgcgctg gtggcccttt ccttcatgcc tgtgctgatg ctgggcggcg gccacggaag
     3181 gagaaagctg tcccggtaac ggggagagag ggaaacgtca ggatgctttc tcgccctggc
     3241 cggcgttttt tgccggttcg ggcgcgtcgt tgtccgctac ctggtggatc atgtccgtca
     3301 gcgcctgcac atactcttcc gtacgggcat ccgtgacggg atacttgctc cggcaggaga
     3361 ccgtgggaat gcccaggacc gcgtaatggc ggtcattgcc tgtgagggcg gaaggttccc
     3421 ggtggaaggt agcgggcgcc tgctcccagg atttggcgaa gggggcggcg cactggccag
     3481 caaatttctt gccgtgcggg gtggtcgtga acagaacggc ttcccggtag gattccgggc
     3541 ccggctgttt tcgttcccct ccggcatcca gcttctgggg ttccagattg atcatgccca
     3601 ggatgttggt tgctccaatg ctgccgctta gggtgctggc atgcacgcag gctccggaag
     3661 agatggattt ccctgcgggc tccgctccgt tcgcgtatgc gacaaaatag atattgtgat
     3721 tggtggggat gtctttcagg cctctcgccg tctccagcag cagggccgtt ccgctgccgt
     3781 tgttgcgctg tccgctggaa tcataattgg ctcccacgac gatgctttga ttgttggaat
     3841 acaggccggg cttgtaaacc aggatattgt aaatggccgt tccttttttc acgtttcctc
     3901 cggatacggg ctgcatggtg acggcgtacc ccatgtccga gaaattcccg gcgatgtatt
     3961 tggcggcggc ttccatggat ttgggctggc tggcgtttct ttccccgatg tctttttgca
     4021 ggacttccgt gtgtttttcc atgcgtgcgc ccagggtccg cggcgtttcc tgccctccgg
     4081 aggaaaacat gggaagaaat gtgcaggagg aaagaccggt ggacagcagc aggaatccgg
     4141 aagacaggac ggcggaggga tgcatgcggg gcatcatggc tttacagggt ttcaatataa
     4201 tgcagttcac gggcaatctg ggctgccagg gggatcttca tgtggtccgg caggacgctc
     4261 caggtatagg tttccacctc caggtgcggt tgcgggcctt tgcggccccg gaggaaatta
     4321 tgcgttttca ggatgaattc ttccgtgctt ttcaagggtg gttccggtga agcgtacagg
     4381 ggaatgtggt aatgaatgcg ccattgggaa tcacgtattt cctctgcata atcccgccca
     4441 taggccaggg cattgggcag gtccgggaaa agcatggcgc cttcccggtg gaggatgctg
     4501 gtctgatgga aataaacgcc gtcgtcaaac tgccggaggc gttccaggtc ttccaccccg
     4561 catatgcggc attccagggc gttggaaaat tgcactttgc agagggcgat gttgttttcc
     4621 tcccaggcgg agagggtgaa ttccggttct tcccggagaa tggcgaaatg gcaggtatcg
     4681 tatgtcagcc ccaggtggcg ttcgataagt tcgggacggc gggaacggtt gcggaggccg
     4741 ttgaaaaaac ggatggctcc atccgtatcg tgaaaatggc cgaagggttc cggttccagc
     4801 cccagctgca tcaggcggcc ggtctgcctg cccagcacat ccaggaatcc gctcatggcg
     4861 tccagccggg agaagatgcg ttcttcatcc gcatgaaacc agctgtgcga ggcagggagc
     4921 gtgctgacgg tcagcctgtc agcctgttcg tcaccaatga gtgccagaat gcggaacagg
     4981 ttgcaggtgt attcaaaacg ttccggtgta gtccagtccg gctggaaaac gcgttctttc
     5041 acgcgctgcc cgtggaaatt tccgtaaggg aacccgttaa gggtttctac gcgcgcgccc
     5101 tggtcttcca gccatgattt cagggtttcc gcggcgtgcg gcgtttccag aagctccgct
     5161 gccgccctgg cggacaggcg caggccgatg cccaggggga gatccttcag aggggagtcc
     5221 atcgccgcga gttcctgcct gatgcgggga acgcaggtga aaagagcctc cctggtttcc
     5281 gcccaggatt ctgcgggatg aatattggta caataggaaa gcatgaaggc gtgtgtgatg
     5341 taaggggaac ggcgtttttc tgcaggatgt ttttcttgcc taaatacggt tccgcgcaga
     5401 aggaaaattt catggagttc cgctttttgg cgtcatccgg gagctttccc cggcaagtgg
     5461 agtgcctgcg gagcctttca acttccggaa tagggtgtca ttggagcaat aaaaaacagg
     5521 ccgcctgcaa gctgggcctg tttttggaag aggaggcatt tgccccggcc cggaaacacg
     5581 ccatggaggc gcaaaagctt cgggccgggg aagattgccg gctgttactg gacgatgccc
     5641 agcggagcag tcgttttcca gtctccgcgg gtgaagtccg ggaacacctg gggcatgccg
     5701 ccctgggcca cggagtattc ggaaagctcg gagacggagg accagaaggc tccttcataa
     5761 acgttctggt ccatcggttc gccattgcgg aggcattcga tgatgcggaa gagcatcacg
     5821 aagtccaggc cgccgtgann nnnnnnnnca ttcatgataa tttccgttgc ccagcttttc
     5881 cccggctacg cgggtcggga agccggccag ggtgcccagg gtgccctgga tgagattcag
     5941 gcgggagtag gggcgcggac tggtttcatc ccattccacc aggacggtgc ggcccatggt
     6001 ggtcttgatg atggaggtat tcatatcgcc gcaggcaaag tccagcttgt tccacttgtg
     6061 gtccgccggg aaatttttct tggcatacgc ggcgcggccc agggccgggc tggagaagga
     6121 gacgagcctg ccgaagcagt cgtccttgcg ggccagattc atgtactggg caatggggcc
     6181 gaggccgtgc gtgggataca cgttgccgtt gcgcttggcg tagtgatagg ttctccagga
     6241 accggtgccg cgttccacct gcttcatctg tccgcgcagt tcatggatgt aggcggcttc
     6301 tccgtacagc aggtcgccaa tgacgccctg gcgcaccatg ttcaggtaca tgagttcctc
     6361 acgtccgtag ttgacgtttt ccatcatcat gcagtgcttc ctggtttctt cggaggtatc
     6421 cacgatttcc cagagttcct tgatggagac ggccataggc acttccacaa aggcgtgggc
     6481 gcccatcttc atcacgtcgc aggtaacacg gcagtgccat tcccagctgg ggcagacgat
     6541 gacggcgtcc ggcttgactt ccttcatcat gcgcttgtag tcttccggtc ctttcgtgaa
     6601 aatggggggg cgcttgcccg tcttcttttc cacgacgtcc gcactgcgct tggcccagtc
     6661 ttcatacagg tcgcaaatgc cgacaatttc cgcaccttct ataacggcca tctggcccac
     6721 gtgcatggaa ccgcgttcac ccacgccgac aaaggccaca cgcaccttgt ccagcttggg
     6781 agccctgaag ccgcccatgt agatggcgcc gggcttgcgc gggggccacg tggtcatggg
     6841 gccgatatta ttcgggtccg gaatggtgat tttcttggga gccagggggg ctacttcctg
     6901 ggcgttggca aaggaaccgg cagccagggc gccagtggcc aggcccaggg tctgaaggaa
     6961 acgacggcgt gatgatgaat tatccatgga tggtaattat ataaaggatt tgaatcccca
     7021 gtttgccata gatactgagg cttgagaatc tttctttcac agatggtgaa aaaaatgcat
     7081 tcctctgtaa aaacggaata tccctgattc ctggaaggaa tcaggaggga atgccgccgc
     7141 cttttgaaaa tcccggacat gaaaaaagcc ccagtcctgc cgcagcgcgg gaaggacggg
     7201 gcctgtaagg tcatcatccg gttgtgccga tgttgcatgc cgttgcttgg gaataaaaca
     7261 ggggagcgag cgtatcgccg ctccgcgttc cgaaggatcg gccctgcatc ggaggacatc
     7321 cggagggtgt tgtttgttgc cgccggcgct tggctgccgt ttccgttccc ggcgacgggg
     7381 aagatcattc cgtttcctgc ggcttctatt caggggaccc tggcaggggg ccgcgtgccg
     7441 tggaatcggg atcatttccc cgatcatccg tagatggtcc ggttattgcg ccatgccggc
     7501 ggcgggaagt tttggcgccg caagcctggc ataggcttca ttcagagcct tgcccagcct
     7561 ggggcggaaa gcggtggtgg cgggcagctg ggtgccttcc atgccgcagg agcggaggat
     7621 gtccgggatg gacggggcca gagtgcgctt catgatgttc gtgcagtctt ccaggtccac
     7681 tccgtatttt tcctccatca ggttcttgat gcggtccgcc atttttctca tcctctcaat
     7741 ctgttttctg gacatgatcg gctggatgat ttcattcggg tcggggacgt agccgatcag
     7801 cattccaatg gcccgtttca gatcttccgg caattccgtt acattgccgc tgttaaggac
     7861 ttgagccaga acctgataac tgctggtggc gttttcgtca taatcatgaa tgcccgaggc
     7921 cagcgccacg cctgcgatgc tctgcacgta gcgggctact tccggggaat aatttccttc
     7981 cacgaaggtg gagggaacgt catccgcgta agcctgcatg gcggcctggc aggcgaggga
     8041 accgacggtg cccgcgcata gtgtcctgat gatgaacgag gttattttca tgattgaaac
     8101 tccggttgaa gcttcccgac tggaacgatt aggagaaccg tttgcagcaa gtgagaccgc
     8161 gctggcttca attccgttca aaagtttttt gaatgattaa aatcatgctt ccgcacggct
     8221 gtcgttcctt ccgtaccctt tccgcggctc cctgcgggga gcggaacgtt tttcctgcat
     8281 ggggctgctc cttttcctgc gcgcttctgg gaccgcgcgc aattcagccg ggacggaggc
     8341 gctggaagcg tgcacgggaa ggaggcgtcc caacagcttg tgcacgtccc tggctttcct
     8401 gacttcctcc ggggaacaga gagcgatggc ttgcccggcg gctccggcgc gcgccgtgcg
     8461 gccgatgcgg tgcacataga cctccggttc ttccggcaaa tcataattga tgacaagccg
     8521 cacgtctttt acgtcaatgc cgcgggcggc gatgtccgtg gcgacgagga caggcgtttc
     8581 cccggaacgg aattcacgga gcatgcgttc gcgcctgccc tggcttttgt ccccgtgaat
     8641 ggcggaagcc ggataatcct ttccggacag gaaggaagcc aggcggtctg ctccatgcct
     8701 ggtccgggta aagataagcg tgcggccagg cagctcctgc tgtttcagca gtttcagggt
     8761 aaggagcctt ttttccgact gggagccgac catgcaaagg aactgttcca ccctgtccgc
     8821 cgtggaggag ggaggggaga ctctcaccgt ttccggtttc tgaagcaggg tctgggccag
     8881 ttgccggata ttgtccggca tggtggcgga aaacatctgc gtgtggcgtt tcctcgggat
     8941 tttggagacg atttttttca gggccggggc gaatcccatg tccagcatat ggtccgcctc
     9001 atccagaatc agggtttcca cgccgtccag actgacggtc cggcgttcca gatggtcgat
     9061 cagtctgccg ggcgtggcta ccagaatatc caagcctccg gccagttccc tgaactggga
     9121 agcatacccc atgcctccga agatgacggc ggtggacaga tgcagccctc tggcgtaagc
     9181 gcggatattt tcatgaattt gcgcagccag ttcccgggtg ggactcagaa taagcgccct
     9241 tacccggcgg cccggcggct ttccggaacg ttctgaaagc tgttgaataa ggggcagggc
     9301 gaacgcggcc gtttttccgg ttccggtttg cgccagcccc atcaggtcct tgttctgcag
     9361 caacaggggg atgcaggctg cctggatggg agtaggggtc ttgtactcca gattctccag
     9421 ttggcggaga atgggagccg acaagccaag ctccctgaat gtagttgata ccatgtctgt
     9481 ggatgtgtct atgaatgcgc ctcggctgca atgaactgac ggaatcacgg caataacgga
     9541 acaaaaaagt ccgcaggagc gatactgaaa agatcaatac ggagacgccc gtattccgcc
     9601 aaccgcaaat gactatcaac aatcaacaac caaacaccat tcagcgggaa cggctttatc
     9661 agagcacggc aaagatctcc tgtcaaggaa aataaaaacg tttgtcctgt tagtgttccg
     9721 tatctctgct tcaaaaacag ttcttgtcat gattttttta tagaaattga atatgaataa
     9781 attaccggat gaagcgggtg aggaatccgc cgtcaaccct aaattctgaa tatgaatgac
     9841 tgattaattt taaggaaaaa ctttcagaag ctgatcagcg gttgtcggat aatctggcgc
     9901 aaggagggga actttctaca taatttgtct tagattgtca tagtaatata aaaatgcgta
     9961 tatatcattc ttgcataatg cacaaataat gaattgccct ggtaagggta aaaaatactg
    10021 gttgtttaac gataaagagc atatttttaa tcttggcaaa ttaatcgcag ccttagtcag
    10081 aaatgctgtt gttatcgtat tttgtaaact aagtgttccc gagatgaaat catttcttga
    10141 cgatagtctt gtgaatatga tactactaaa aaaagaggaa tggcgtcttg ttgtttgacc
    10201 aaaattgaaa aattctaaat atttaattat atgaacatag aacatttggc aatgtacgtg
    10261 cttgatcttg aaaatacaaa agatttcttc gttcgttact tcggagcgaa atccaatgaa
    10321 ttatatcata accgtgaatt ggatttcaaa tcttacttcc tgtcgtttga cagtggtaca
    10381 aaattggaga taatgactcg tcccggactc aacaatgata caaaaaatcc gattcgatgc
    10441 ggatacattc accttgctat aagtgtagga agcaaagaaa aagttaacga tttaacagac
    10501 ctccttcgta atgatggtta tactgttatc agtagtccac gcacaactgg cgatggttgt
    10561 tatgaaagct gcattcttgg tcctgagaat aatctgattg aaataacaat atgaaaaaga
    10621 ctgataggag gtgttgccat tatttgtatc actttggctt cgtacggaac tgttttatct
    10681 cctggatgat gattctccaa taatagaaaa caacaattca tgtatggaaa tcagcaacat
    10741 taaactcggt ggcgttcttt ttaacaaggc tcaaaatctc aatgaaggga tagtggcgct
    10801 tggtaaagat acactacgca tacaggcagg ggaaaaaacc gattttttct gtgaccccca
    10861 aggaaaagct actaatacaa cagccccggt cattcattcc aggatagata atacccgtcc
    10921 ttttaccttt acagtaaaag ttacacccga atttacgcca aatggcacgt attctgccgg
    10981 agccttattt attttcgtag atgatagcca ttggcagaaa ctttgttttg aacaggatga
    11041 agacggtaat caccgaattg tgacagtcag aactgtagat acttccgatg acaataatca
    11101 tcagcagatt gaaatttcgt cagtatatct tcgtatgtca tctgataccg aagttatagg
    11161 taattattat tcagaggacg gtaaaaactg gcatcttgta aaaatatata agaatgagta
    11221 tcctgaaaag ctctatctta gcctgagtgt tcaaagtccc agagacaaat gtcatagttg
    11281 tttattttcc gaggtgtctt tatctgaaaa gccagtgcgc aattttcgta atggacagat
    11341 ttgacctaag ttaaaaaaat ttgaggcctg agctgttgcc aggcatgatg atcctttaaa
    11401 tgtttttgcg gaacgcaatg tcaaggacag gtaaggcggc ttgagagccg tggaggcgtt
    11461 catcttccgg cagagaggat ggacagcttg aacgttccaa ggcttcagca tgtgcaggaa
    11521 gggcgacagc ggaagattcc gcatgcattc tttcatacag gaacccggcg gaatgaaatc
    11581 atgctcccgg tatatgaaat gtcccggaga atgaacaatg ttgtctctgt ctccggacgg
    11641 atgggggaaa aggcaatttt caacgctgca ggaatttgcg ggcgcccgcg cagtaaacgg
    11701 ctccggaata aacggtcagg atgacggaac cccacagtgt aacctggtac agcaagttgg
    11761 aagcgccgga accgtgccac aggcagagcg cggccagccg gtccgtgatg gaaggcaggt
    11821 attccggcgt atgcgtccat gtcagggcca gcaggcaggc gatgcagaag gccagctgga
    11881 aggcggtttt ccatttgccg gacgttcccg cagggatgat gacgccatga tcctgcgcaa
    11941 tctggcgcag tccggtgacc aggaattccc tgctgatgat gagaatggta atccaggcgg
    12001 ggcagagccc ttcgcccgtc aggtaaatga aggcggcgga gacggctatt ttatccgcca
    12061 gggggtcaat cagcttgccg aaggtggtga cctgattcag gcggcgggcc aggtagccgt
    12121 ccagccagtc cgtgcaggca gccagaatga acgtgaccag cgcggccagg tatccccagg
    12181 aataccggtc cgcgacgctg actgctgccg taaagacgac taccagggca atacgcgtca
    12241 gggtaatggc attgggcagg ttcagcatac cggtttatta ggggacagat ggctgcccgt
    12301 agcaagcttt tttgctggat tcaggaatga aatttccgga aaacggcgca cggaaaaaag
    12361 cgaagggaat acgaagatac gggcagccgg gcgcatgatg cgtgcgtttc agttcctgct
    12421 tcccgtttcc gctgttttgc ggcaattaat tcccgatcat tttgtagaag tcttcctcgg
    12481 aaatgacggg cacgcccagt ttgagggcct tgtcccgctt ggagcctcct ccgctgcctg
    12541 ccaggaggca ggaggtggat ttggagatgg ctccggtggc ttttcccccg tgggaggcga
    12601 tgagctgttc aaagtaatcc cgcggatggc tgagcgttcc tgtgatgacg aaggttttgc
    12661 cggacaggga gcccgccggg atttcgagaa ggctggcccg gtaagattgg gattggggat
    12721 tgatgcccag tccgtgcagg acgtccatgg tatggttccc tgctgcggag gcgaaaaagc
    12781 tccggaggga tttggccgcc gccacgccga tttcggagcc atagctgaat ttggccgtat
    12841 ttttacgcag gtagcccgtt gccagatagg gtgcggtcag gcggtcgatt tcctccatca
    12901 gttcctggtg gcgctcctgt accgcctctg cggaaagcgc gccttccttc accgcctttt
    12961 tgttttcgcg ggtgttgggg ttggttttgg cggcctgttc caccagttca tccaggcgga
    13021 cgatgtcccg gaggtaggga gaatccgcca catgcttcag gtcgggatgc gtgtccgcca
    13081 gcgccttggc gacaacttcc cccaccaggg gaatgccgaa ggcgatgagc cagcgctcca
    13141 gaggaagccc gcgcgcgttt tgcagggcgt ccagagcttt ccgggcattc ttctccccgt
    13201 aacggcgcgg ttcttccggc gtgcccaggt tcaggttggc gagctggtcc ggcatcaggg
    13261 agaaaaggtc cagcgcggag gacgccagtc cggagcttct cagcgcttcc gccacggaag
    13321 agccgatgct ttccacatcc agagcggacc gggaacagaa atgggtgatg cccgtgacgg
    13381 cctgcgcggg gcaggtgaag ttggtgcagc gccaggctac tttcccttct tccttcatga
    13441 tggggttgcc gcaggcgggg cacagccctt tcgtggcttc caggatgctg tagggcctgg
    13501 cgtcctgcgg gcgtttggaa aggttcactt tcagaacggc cgggatgatt tcccccgcct
    13561 tttccaccag caccgtatcg cctatgcgaa cgtccttccg gtcgatttca tcctggttgt
    13621 ggagggtggc ccgcgctacc gtagaaccgg acagcagcac aggctgcaat tccgccacag
    13681 gtgtcagcac cccggtgcgt cctacctgca cgacaatatt cagcagggtg gtttccttct
    13741 gttccggggg gaatttatac gccgccgccc agcggggcgc gcgagccgta gctcccagcg
    13801 cttcccgcgt agccgtggag ctgattttaa taacggctcc gtccgtgccg taggggagcg
    13861 tatggcggag cctgtcaata tcccgcacgg cctggcgggt ggattccagg ctgtccgtat
    13921 aatacaccgg ctcattgcag gggatgccgc aatggcggag catgttccag aagtcttttg
    13981 cgtccctgag ttccgggcct tcatacgcgc ccaatccgtg ggccaggaag gccagcggac
    14041 gggtggccac ctgccggggg tccagctgct tgagggttcc ggcggtggcg ttgcgcgggt
    14101 tggcgaatgc ggggagcccg tcggcgtccc gttcttcatt cagtttggca aaggcttcat
    14161 tgggcatgaa gacttccccg cgcacttcca gcacgggcgg cgcgccgggg gggagggtga
    14221 gggggacgct gcggatggtg cggacgttgg ccgttacgtc gtcccccacg tctccgtctc
    14281 cgcgcgtggc cgcataagcc agtttcccgt tgcggtacat gatggaaagg gccactccgt
    14341 caatcttggg ctccacggag acgggcacgt tctccgtttg cagcgccnnn nnnnnnntcc
    14401 gccagttctt catccggtac gggggcgtcc cgctgctcaa aaatgtcgtc aatagacaac
    14461 atgggcaccg catgtctgat ctggttgaag ccctggagag gggcgccgcc tacgcggcgc
    14521 gtgggggagt ccggatccga cagttcggga tgggcttttt ccaatttttc cagctccagg
    14581 aacagggcgt catattccgc gtcggaaatc tccggttccg cctgttcgta atacagacgg
    14641 ttgtggcggc gcagttcgga acgcaaatag tctatgcggc ggtgagcggg gtcctccgcg
    14701 tttccggggt ggtcggtgcc ggcattttcc cgcgcctccc cggcccgggg tgccgtttca
    14761 tttgttacct gctgtgaagg ctgtttgccg gctgccaggg aaaaaaggtc ttcctccata
    14821 ggaaaaatga gtggggaaaa tggctatttg ctcagtttga tgacggaaca gggagttttg
    14881 gaatattcat gagccttgaa caggaccgtt ccgttgacga cgggaagggc ggtgttgtcc
    14941 cccagaagca atgctttgga atagccggcg ggcagggtga cggctgttcc cccgtcattt
    15001 tcgtagccgg tggcatccag cggctgcatg cgggcgtgtt ccgctcccgc cgtcaattct
    15061 ttcatggttc cttcactggg cggctgggcc ggctccttgt ccggcggggg aatcaggaag
    15121 acgaagatat ccccctgccg attgatggtg gcaggctggt ccagctgcat gaatgcccgt
    15181 gttccgtaca cggcttcacc gttgatgttg agccatcggc ctacgccgcg catggtggcg
    15241 gccacttttt ccggaatggt gccgtccgcc atgggattga cgttcagcag aagattgcct
    15301 cccttggtaa cgcattccac cagtttttca atgatggttt ccggagattt gatgttggtg
    15361 tcgtagcggt tgtagcccca tttgtcgctt accgtcatgc aggattccca gtccacgccg
    15421 ggatatccgc gccgggggat gaagcgttcc ggggtaatat agtccccgca gcgcaggtcc
    15481 agcgtgccgg cctggttttt gttcaggccg gaataagcgt acaggcggtt gttcatgatg
    15541 acgccgggat tgatggagcg caccatgtcc atcagttccg gcgccttcca gcctttggcc
    15601 ccctccatgg ctccttggga gtaatcccac cacatgatgt cgatgggcgc gtaacgggtc
    15661 atcagctccc ggacctgggc gtgcaggtat ttctggtagg cggcgtggtc gcggggaatg
    15721 tttttcctgt taagcattgc cgcctgtccc gcagggtagc agaggtccgg gcagatggtg
    15781 ttgtcatagg acgggtgatg ccagtcaata acggagtggt agaggcccac tttcaggccg
    15841 cgcttgcggc aggagtccac atattcgcgg atgatatccc ggttcacggc gcttttggcg
    15901 tcgtaatccg tcaaggcgct gtcaaacagg ccgaagcctt catggtgctt gctggtgagt
    15961 actacatatt ggcagcccgc atcccgggcg agctgggccc attcttccgt caggccttcc
    16021 ctgggcttga acaggggcag agcttccgcg gcgtaggtat ccgtatccac ttccacgttt
    16081 ttttgaatcc attcggaacc tccgggcctg cctttccatt ccccggccaa tccggaatac
    16141 aggccgtaat ggatgaacat gccgaatttg gcatccctcc accattccat gcgccgattg
    16201 cgttcccccg ccgtttccct gacagcagca tggttggcat ccgcggggag ggggaggccg
    16261 ggcgctgctg ccgaaaattc cgcgggaacg gtggccgggg agccctgctt cattccgccg
    16321 tcgcgcagcc gggctgcgaa ctgttcgtcc agggccagcg caggctgtcc gttttccgta
    16381 atgattttag tggcggactg ctgggccggg gcgggaagtc ccagagtgac ggccagggcg
    16441 gcaatcgtgg ctatgttcat ggttgttcag aatggtgtga ggctgggggg aaggctgaac
    16501 ctgcttaaat gacgtccgta tccgcgtagc tgcccagaat cttcagcatg gagcagtgct
    16561 gctccagctc cggaagggtt tcccgtaagg gggattcctg aatgtggccg gcggcatcta
    16621 tgtaaaagac gtattcccag ttgatgactt tggacgggcg ggattcaatg cgtatcaggt
    16681 tattgttgtg ccgctggaag cagttgacta cttccgccag ggtgccgggt ttatgctgga
    16741 tgcggatgag cagggaggtg cggtccctgc cgctgggctg cgtttcctgg cgtccgatga
    16801 cggcaaaacg ggtggtgttg caggactggt cctgcacgtc ttcttccagg atgttcaggc
    16861 cgaacaattc cgcggccagc ggacagccga gggatgccgc gcccatggcg gcgttctcct
    16921 tggcaagaat gctggctttc gtggtggagg acgtttccac gagttccgcg tttgggaaat
    16981 gccgctggat ccagttccgc gtctggccga gaacctgggg gtgggaatac aggatgcgga
    17041 tgtgttcccg cggaatatcc gccatcaggc tgttgcggat gttcagattg atttgggcgc
    17101 agattttgag gggagaggtg acgaaaagat ccatcacggc ggtgacggaa ccttccgaag
    17161 agttttccac gggaactacg ccgtactggg ccttcccccg gtccaccatg tcaaatacgt
    17221 ccttgaagct ctggcacgga atgagttcgc aactttttcc gaactgcttg agggccgcct
    17281 ggtggctcca ggttcctttg gggcccaggt aggcaatggt caggccgcct tccagaaaga
    17341 aggagcagga aatgatttca cggtaaatag cctggaggga ggcttccggc agcacgcccg
    17401 cattcaggcg gttcaatttg ttgagcaggg ccgtttcccg ttcgggaacg aagatggggg
    17461 aatttttttc ctttttaatg cggccgactt catgcaccca ttcggcgcgt ttttttaaca
    17521 gctctacaat ccgggcgtcc acctcgtcaa tggccagacg cgcctttgtc agggcgatgg
    17581 tctgttcatc cgtgtttccg cttttttgct gcctgggggg attgtccgga gaggcgtcct
    17641 ccccgtggtg cgtggtctcc atgtctctgt gaaagctgtg tggttaaagc cgtgtcaggg
    17701 tagcggttcc gggggggcaa atcaagatta aaggggaggt ctccgccgcg gattcttctt
    17761 gcatgcgcgg aagatgccgg gcaggttcat ttctccttga ttgatgaaaa gagtattgtt
    17821 cccggctgac tgtgaccgtg gacgctcctg ccggaaaggg ggcgtaagaa ggtgaacggc
    17881 gggtctgttg ccgcatcttt gtccgcaagg atgggggcgc cggatgacgg cggattgctg
    17941 acgaaggccg ggccggctct gggatttgaa tatggcgctt tcccgtattc tgtccgttga
    18001 agcggcttcc gctgccggga aatggcgggg aggtttagtt ccgtcccccg tcttcctgta
    18061 ccgggcgtgg ctccgggatg gccccgtaaa gcccgttggg gaagagctgg agccacggcg
    18121 tgtattccgg cgggacgggc gcttcgaaat ccatgcgctt tcccgtgcgc gggtggtcga
    18181 agcccagccg ccacgcgtgg agcatgagcc tgccgggttt tgccttttgc cgggcgggtt
    18241 tggcgtaaat gggatccccg atgagcggat ggcccagatg gagcatgtgc acgcgtatct
    18301 ggtgggttct gcccgtgtgc agcgtgcaca gaaccaggga ggagtccgtg gacgggtctg
    18361 cgcagagcaa atcgtagtcc gtaatggcgg ctttcccgga accgggattg actactgcca
    18421 ttttcaggcg attgacggga tggcggccga tatgggtgaa gaccgtgccg gaactttggg
    18481 aaggaatacc ctggacgacg gccagataac gcttttccgt gcttcgggcc gcgaactgcg
    18541 ccgtcaggga ctggtgggcg ccgtcatttt ttgcgatgac caggcagccg gaggtgtcct
    18601 tgtccaggcg gtggacgatg cccgggcgtt ccactccgcc gatgccggag aggtccccgc
    18661 agtggtggag cagggcgttt acgatggtgc cgtccgggtt gccggccgcg ggatgcacga
    18721 ccatgccgct ttccttgttg atgactacca cgtcttcatc ttcatacagg atgtccaggg
    18781 ggatatcctg aggctgggct tccgccggtt ccggttccgg aatgcggacg gtgatggaat
    18841 caccgcggga gacggggttt ttaggcttgg cgggagagcc gttgaccaac acgtcaccgc
    18901 ttttgatcag gctctggatc ctggagcggg aaagctccgg aaggtgggcg gcaaggtatt
    18961 gatccaggcg gattttcccg tcggcgtcgt ccgtagtcag attcatgttc cgtggcgttt
    19021 ggtcaatggc tgtaggcgga gggcggcagg tcaataaagg tataatccag cccgtaaaca
    19081 tccttgagaa gaagccccag ggcctttacg ccgaacgtct ccgtaaagta gtggccgccg
    19141 aagaccagat tgacgcccag ttcctgggcc agggggatgt tccagtggga gccttccccg
    19201 gtcacgtagg tgcggcagcc gagggccgct gcctgggcaa gatcgtctcc cgcgcctccg
    19261 gtacagatga agatatctcc ggccggagcc tccggggaat ctttccagaa ggcctgcacg
    19321 ggcgcgccga ggacggtttc cagtttttgc tccaattccg cgcatgtacc tggaaattct
    19381 ccgtgcgtac cgagggaaac gccgtggtag tccagcccat catgggaaca tggccggagg
    19441 ccgcaggcct tcgccagcag ggcattgttg ccgtatgcgg ggtggacgtc cagaggaagg
    19501 tgggcggcgt aaatcgccag gttcccgtcc atggcggctt tcaacttccg gtaggcgatg
    19561 ccggtaatgg gctgcatggg ctgccagaaa atgccgtggt gaagaaggag caggtccgca
    19621 cctgtttcca aggccgcatg aatggccttt tctgagccgt ccacggcggc ggccaccttg
    19681 gaaacggtgc ccccgttttc cagttgaagg ccgttcaggg cgcggggggc gtccggaatg
    19741 gaattcacgg agaggattcc gtccagaaag gagatggcgg tgtgcagctc aatcttgctc
    19801 atgcgggagg agtggttagt tgtcttccga aaggctggcg ccgagcagac gtacggcgtc
    19861 catgttcagg ataaaataat tcggggttgt atttactctg ccgtaataaa atctgctgta
    19921 tccggcctct cccgccgggg cgatttccag cgtgatgact ttgtcctccg tttccggttc
    19981 cggggcaaag gtaatgtcgc tggaaccgga ttgggactgc acgggggccg cctccttgta
    20041 agactggagg atgagtttca ggcggaaaac gggatttttc agggcttcat gcgctgtggc
    20101 gtccgtatag gggagccagg tgctcacctc cattttttcc agttcatcca gatagcggtt
    20161 ggcgcggttc gggttgatat tcaaggtttc atccttcccg tctttggagg ctgtccaaga
    20221 atcgtccaga cggtcgaatt tcaggaccag ggggcctccg gaaccgatgc gttccaggtg
    20281 catttctctg aggtcaaacc tgctgaagga aaaaagcctc ttttttttcc aggccagtgc
    20341 atccgtcagc aaatccttca tgtaggcgtt gtccagtatg gcgacggtgg gttttccggc
    20401 ctggcgggca taccaggaac cgtccgcacc cttggagaaa aacaccgtca cgggggggcg
    20461 tggctcttca ccgggcttgg gcagaaggga catggtgagc gtcattctgg gattatccag
    20521 cccgtacacg gaaaggtccg taactttgtc cgaagcgaag ccggccactt ttccctgttt
    20581 aacggttttc agcagggaaa tgagatgctc cggattagct tcctggtaac gggcgccctc
    20641 tgccgtgtac agccaggtgg gcttgatgcc ttttttctca tccccctgaa tgaagcgggc
    20701 gatggtaggg taatcctgaa aacttttacg gatggaaagg ccggtaatct tgtaatcccc
    20761 gatgtccgtc aattgcttgg aacgcagaag gcccagatcc agcggaatat tgcgtacgcc
    20821 gggaacttcc ttgttggttt ccgtcgtttg cgccaattca aagacggcct tacggtcgga
    20881 aacggtggct ttgaccagtt cggacaggct tccgttgtcc gagggaggat aaattcggag
    20941 cgtgacgggc tgttcctcca tttgcaggga tttgccgtcc cctgaaaggc ggcctgtgaa
    21001 attcctgatg gagacggagg tgatttgatc atccgtcata tccggcacgc tcgtttcctc
    21061 cgggttgtac acgcgcacgg cggtcagctt ctggagcgtg cccagcagca catccatcat
    21121 ggccgggtcc gtatccaggg agaggggata cgtgattttc cagggggagc taggagtctt
    21181 gcgctccagg gcgatgcgct ggccccggtt gttgatttca atggaggcgg gcatggccgg
    21241 atgcagtagc aggggacggt ggtcccgaag ctggcggatg ccggatttga acagagggag
    21301 gatgttgccg gtgcccacca gaatgcgggt gtcccggccg taaaaatccg tctgcatata
    21361 ggtggtgtca tccgtggtct tgttctccgg attgtccacc agccagggcg ccggggaacc
    21421 cagcgtatag cgcgccacag tggaggaacg ctttccgtcg tcgctcattt ccttgagagt
    21481 gatggtaacg ggagtggttt tcacgccgaa ttccttcagg gatgcggaat caatcttgtg
    21541 caggggcaat gcgtccacga tgctggtgga gtacgtgtac tggagaatgg ccgccgccgc
    21601 gcgaggatcc atacggtcgt tccagggctt ttcgcaccac cagacgccgt tgggtttgcg
    21661 gcggaattcc gcgacatctc ccacgcaatt gatgcgcatc caggaaacct cgttcatttc
    21721 ttcccgggtg tacgggaaaa gccgttctcc ggaactgaaa gcggtgcgcc cgatgatgcg
    21781 ggacagattg ccgtcaatca taaggagcac cgctgctccg acagaagcga gggtgatgag
    21841 gatgagaaga ataaaacgga atgtgcgcat gtgcttccgg taatgggcgt gggtggagtt
    21901 aatgacggcg cgtgttccag ataatcaggg cgatcagcag cgccagcagg gggagtccga
    21961 tattcgttag gagttccaaa acgcccaggg cgttgcggtc cagattcagc ttgacggtca
    22021 ggtcatggcg ggagccgagg ccgcgcaatt cttcacggtc cgtcatccag ccgatcagcg
    22081 tacgcatgaa atccctctgt tccggtttga tttgccgggg ctggagcagg tccgtattgc
    22141 caagcagaac caggcgcccg gtagtcttgt tctggttcac gtccccggca ttcccccgga
    22201 tgagggccgc gccgatcatc agagggcccg gattatcttc ccttgcgtcg aactgggcag
    22261 ggaactggtt gtatttggtt tctccgtaat aatcctccgt tgtgaccagg agggggtatg
    22321 gcgtaatgcg cttctgttcc atggccgcat tgtcggaatc cagaatgagg gagatgctct
    22381 ccccttccag tccggtgctg gaattccaga attcacgggt gcaattcagg gagggggcga
    22441 aaatggagtt aatttcaaaa acggaacggc tgcccctgtt gcggagcatc acgcggtcat
    22501 tctggggccg tagcccctgt tcgcggagaa agcggtaaag ctgtttggga gtgtcattct
    22561 ggggttccag catgatcaga atggcggcgt tcgggcgcgc ccagtaccgc tggagcactt
    22621 ccgcctcctg cggggacaga tcatatttgg aaccgataat catgaatcct gcggcgtctt
    22681 ccggaatttc ttccagaccg ctcatgcgga tgggaacaag ctgcaggttg acggaacggc
    22741 agattttgcc cagcgtcgtg aaaatgcttt cttcctggtt gttgctcagg gactcgttga
    22801 agttgctctt atccgccgcg atataaattt tacgaggttc gccgttggcc gctccgtaaa
    22861 tgccggcagt catcatgtct tcaatctgga gcgccactgc cttcatgctt ttgccgtccg
    22921 gcccgggtgc gtatactacg aaggcgtctc cgggcaggat gcgcacgtgg gcggcgtccg
    22981 cctgggtgcc ttcaaacgtc ttgagcgctt tttccgtatc ctcccgggca tcaatgatga
    23041 ccaggttctt cttgaattca attccgtaga tattggagat ttcacgggcc ttgttcggct
    23101 ggcggagggg atccacatac tccaccttca ccttgccgtt ggaataacgt tcgtactcct
    23161 caagcagaga acgcatgcgg gtgtagttct gcgtggtgcg caggaaagcg aacgtgattt
    23221 tgacgggggt tttgcgcttc tggatttccg gagactggag catgttgatg ctctgacggg
    23281 aaatttcata acgctggtct tccgtcaggt cccggcggta atactcgtgg cagcctacgt
    23341 aattcaggca tacgacgata aggaacagca ggaaaagctt gatccaggtc atccaggggc
    23401 gtttgacctt gcgggcggcg ggctgggagg cttctgcggc cgcggcgggg gtgtctggtg
    23461 tttcgctcat agataaaaat gaaaagnnnn nnnnnntcag tgtttccagc ggcggtggtc
    23521 aatgatgcgg atggtgacgg ccagcgtcag gactgtcatg gtcaggtaaa acacgacggg
    23581 gcgcgtgtca accagcccgg cggaaaagcg gtccagatgt tccgagcagg acatgtagtg
    23641 gaaaatcccc gctgcgggga attcccccca caccacggga atcagaccca tgaagaaaat
    23701 caggaccagc aggccgatgg tgatgatgcc ggaaataatc tggctgcccg tcatggagga
    23761 ggcgaaaatg ccgatggccg tgaaccaggc gccgaccagc agcagaatgg catatgcccc
    23821 ggcccagtcc gccaggcggt aaggcagaac catgcccgcg tcatatccgt aaatccactg
    23881 cacaatcagg ttggaccagt ccgccaggat gggatagaga agcatgggca gccacaggat
    23941 gatgtaaaag gcgtatgcgg agaagtattt tcccagaacc acctgccatg tcttgatggg
    24001 cgtggtgagc agggattcca gtgttcccgt cttttcctcc tccgccaggg aacgcatggt
    24061 gagcagaggg aagatgaaca ggaagtaaaa ccagaacatg ggagaatgca ggatgaagaa
    24121 caggatgcct tccttctgcg gggccagttg aaaagccttc agcgtgccgg acagggagac
    24181 gctctgaagg gccatgacga aagctaggat aacccatccg tacgg
//
LOCUS       DAWEMX010000025        23693 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_886_length_23693_cov_13.297995, whole genome shotgun sequence.
ACCESSION   DAWEMX010000025 DAWEMX010000000
VERSION     DAWEMX010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 23693)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23693)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23693
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_886_length_23693_cov_13.297995"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2947..3043
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14575..14584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5046 a         6306 c         7059 g         5175 t
ORIGIN      
        1 gcccattgct cagtatccgg acatcgctcc cccggtcatc accctgcgca ccacatatcc
       61 gggggcttcc gcccaggata cggaggaagc cgtgaccgcc gttattgaaa aggaaatcaa
      121 cggcgcgccc ggcctgatgt acatgtccgc caccagcagc tccgacggaa ccgtggaaat
      181 agccgctacg ttcacgcagg gcacggatcc ggatattgcc gccgtagaag tgcagaacag
      241 gctgaaaatc gtggaatccc gcctgccgga atccgtccgg agagaaggcg tgttcgtgga
      301 aaaatcatcc aacaatattc aggcgatgat ctctctctct tctacaggca gcctgaatga
      361 tacggagctg ggggaatggg cttccgcccg catcatcccg gaactgaagc gtgtgaaagg
      421 cgtcgggcgt gtggagcttt tcggtgcgga gacatccatg cgcatctggc cggacccgga
      481 aaaactggag gcgctgggcc tgacccccac ggatattgtc aatgccgtgg ctgcccagag
      541 cgagcgcatc atcatcgggg acatcggggg ggcgtccgtt cccgaatccg cccccatcaa
      601 cgccggcgtg gtagcggagg acgctttccg tacgccggag gaattcgcct ccattgcgtt
      661 gaggacgctt tcagacggtt cctccgtcaa actgggggac gtggcgaggg tggagctggg
      721 cgccaacagc tacgcgttct tttcccgctg caacggccag acggccaccg gaatggccat
      781 caagatggct cccggctcca atgccgtgga aaccatgggc ctgctcaagg ccaaaatgga
      841 tgaactgtcc agggatttcc cgcccggagt ctcctaccag attccctatg aaaccaccca
      901 cttcgtggaa atctccatcc ggaaagtcat ttcaaccctg ctggaagccg tcctgctggt
      961 gttcctggtc atgttcctgt tcctccagaa cttccgggcc accctgattc ccacgctcgt
     1021 cgttcccgta gccctgctgg gaacctttgc cgtgatgtgg ctctccgggt tctccatcaa
     1081 catgcttacc atgttcggca tggtgctcag catcggcatt ctggtggatg acgccatcgt
     1141 ggtggtggaa aatgtggaac gcatcatgat ggaggaaggc ctggatgccc agcgcgccac
     1201 ggtgaaagcc atgaagcaga tcggtggggc catcgtgggc atcaccgccg tgctcgtggt
     1261 ggttttcgtg cccatggcct ttttcagcgg agcggtgggg aacatttacc ggcagtttgc
     1321 cgtaacgctc attgtgtcca tatccttctc cgcgtttctg gccctttccc tgactccggc
     1381 gctgtgcgcc gccatgctga aaccctcctc cgtggagcac caggagaaaa aagggttttt
     1441 cggatggttc aaccggacga tacaccggtc cacggaccgg tacggccgtg ccgtatccgg
     1501 aatgatccgc cgccctgtgc gctccctgtt cctgtatgcc ctcattattg caggggccgg
     1561 ctatttgtac cttacgctgc ccacctcctt ccttccggaa gaagaccagg gcaacttcat
     1621 ggcgatggtg tccctccctg ccggcaccct tcagaaggaa accagcgtgc ggctgcggga
     1681 agtggaggat tacctgatgc ggcatgagcc ggtggagcac gtttactccg tgggagggtt
     1741 cagcttcatg ggttccggaa ccaacatggc catgctgttt gtcggtctga aaaactggga
     1801 cgagcgtccc ggagaaaaca gttctgtcca ggcgattatt gaccgtgtca atgcccgctt
     1861 tgccggagac ggccagatga tggtcatggc cctgaacatg ccggccctgc cggagctggg
     1921 caattcgtcc ggattcgact tccggctcca ggacaagggc ggcctgggat atgcccgcat
     1981 ggcggaagcc cgggacgagc tcctggcccg tgccaacgcg aaccccaatc tggcgggcgt
     2041 ttatttctcc ggccaggcgg atacgccgcg tctgcacgtc tccattgacc gggaaaaagc
     2101 cttttccatg ggagttccca ttgcggagat cagcaattct ctggcggtca tgttcgggtc
     2161 cagctacgtg ggggatttca tgcatgacag ccaggtgcgc cgcatcatgg tgcaggcgga
     2221 cggaaaaagc cgcctgaacg gaaacgacat tgaggacctg catgtccgca atgaccaggg
     2281 gaagcttctg cccctttcct cctttgtcag gctggactgg acggcggggc ctccgcagct
     2341 cacgcgctac aataactatc cctccttcac catcaacggc tcttccgcgc ccggcaagag
     2401 cagcggagac gccatgaacg ccctggaaca gattaccgcc tccctgccgc agggcgtggg
     2461 gtttgaatgg acggggcaat cctatgaaga aaagctggcc gggtcccagg ccacgctgct
     2521 tttcggcctt tccatcctga ttgtctttct ggtgctggcg gccctgtatg aaagctggtc
     2581 cattccgttg gccgttattc tggtcgtgcc cctgggactg cttggagccc tgctggccgt
     2641 cttcctgcgc gacatgccca atgacattta cttcaaagtg ggcctgatta cgaccatcgg
     2701 cctttccgcc aagaatgcca ttctgattgt ggaagtggcc agggaatttt accgggaggg
     2761 catgaatgcg gcggaagctg ccgtcgccgc cgccaaactg cgcctgcggc ccatcctgat
     2821 gacctccctg gcgttcgggg cgggcgtcat tcctctggcg ttcgcccatg gggcggcggc
     2881 cggagcgcag cgcgccgtgg gcaccggcgt gctgggcggc atcattacgg ccacgctgct
     2941 ggccatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgcccc tcttcttccg
     3061 cctgacggcc ggaaagaggg ggccggatca tccggaagga atctgaggca ggattcccgc
     3121 tgcggaaaat ttttcgtgaa cgccgccgga actcctgccg tcctgctgcg gcaggagttc
     3181 cctgcatgcc gcgcaagaga actattgcgc agacgcattt cgaatccgct gtcaatccta
     3241 ttattttagt aggaaatata ttttttcaat gccgccatgg aaacctcatg gtttgcatga
     3301 ataaaatata ttgatgataa aatagttgta tctctttctc atggttctcc ggataccggc
     3361 tgccgctgtt ctccgggggg atgacgcgcg cctgccgttt ccggaggatc tattttggaa
     3421 taagcgggat attttctaaa gctttctctg tcaacgcgcc atttgaacag cggattcgaa
     3481 atccgttact tagcggcctt cccaatatta acaattctta ttaaaagata tgagacttac
     3541 tcttccatgg ccgttgcgcc gttttttgct gggggcgctt ctgtgttccg taacgatggc
     3601 gagcgccgct tccggggcgg agttcgtggt ggacgcctcc caggcaaccg acccgtccgg
     3661 gaacgtgtac cgcacgcttc aggaattgtc ctcttccggg gcgctgtctt ccggggatac
     3721 cgttatcctg tgcaatgatg attcttccct gacctcagcc ctgaatgcgg ctgtccattt
     3781 ccgttccaat gatccggagg tgtcccgcac catcagcggc tccccaaatt atttgtacaa
     3841 ctttcaagga atgcccctcc ggcccggcag ccagacacgt cccgatatat cgggagttct
     3901 tgatgaaaat ggagttgccg ttttgacgat ggattccatc attctggatg gaatggcgtt
     3961 aacaacgacg aatacttctt cctgtataaa aataacgggc aacgtccagt ttgagaattc
     4021 cctaggagtt gctgccatca cctccggaaa atacagcccg gacggagccg tctcgatgac
     4081 gttggaaggc cgctttgttt tcttaaataa cggtcatgct ctccgtattt tagaaaaatc
     4141 aaacgtggtg ataggagaca acgccctgtt ccggagaaat tatcacggct gtattgatat
     4201 gcctacgaat tatgacggag gtgcgatttg gagtaggggg ggaaatatca tcataggaaa
     4261 tcatgcggaa ttcgacggga attatgtttt ctctgatacg caggcctgcc ggggtggagt
     4321 attttatgat gacctttttg ccggtgcgat aactgtagga gcggatgccg tttttaccag
     4381 taactttgtt ttcagtacca actacgctgc tgctgggggc gcaatgtttg tcagaaagga
     4441 tggactgaaa tatgcggcag aaacaggggc aataaccata gggcccaacc ccagtttcat
     4501 caaaaatttt gcttactccg gaagggctga tgcttatggc ggtgtgattt acagtgatgg
     4561 ggacatttct ttcagcgatg gaggcatgtt catcggcaac tatgccaaga cctgcggggg
     4621 agccatcacg atgggaggga aatgcgacct gctcgctctg acgaaggacg cgcttttcag
     4681 cgacaacatg accggtggcg tcttcatcaa ccattacgac ggcaccttca ccgtggaaaa
     4741 cggcattgcc aatgccattg acatgaacgg ttctttgttt tcttcacttt atacgcccaa
     4801 tctgacgctt gccgcggaag aggggcgtga aatccgcttc aacgacccga ttaccagccg
     4861 gaacgaagcc cccggccgct ccatggccct cacgctcaac cgctacacgg acggggaggg
     4921 gaatgttcac gacacggaag ggacgatcgt cttcagcggc gaactctacc agggggaaga
     4981 agagcatctc tccgccagcc ggtacagcag cttcctggcg gataccaccc tctatggcgg
     5041 cgccctcctg ctggaacacg gcgcggtctt cggcaggaat cctgaggaaa taacggataa
     5101 acgatacaac tcctccctga cggtggaaaa gggcgtgctg gaaatcaccg gaggaagcaa
     5161 ggccaacgcc accagcttca ccctctccgg ccctggcgcc gtcctgcgcc ccggggacag
     5221 agctttcatc agcgcccaca acgccgacct ctcccgcggc ttcaccttcg acatgcgcca
     5281 ccagctccag gccgggccgg ccttcggccc gggcctgatg ctctccgctc aggaaacctt
     5341 cacggccggg ggatacatcg gcgtggcgga cacggaagcc aacgcccccc ggttctacgc
     5401 ggaccgctcc tggaagcagg accgcgtctt ccacgtgctg acggatgtgg agcacaccca
     5461 tgagggggac tttgacgggg ccgtctccca ggccaccgga acggcccggg tggatgaccc
     5521 ctacgcctac acgggaacct ggagccaccg gtggacggac gcggacggcg acggctacgc
     5581 cgaacagctc cagctggtct ggaaagcgga cggaaccccc atcagccgcg ttgacccgga
     5641 actggccggg gaactggccg tcaactccct ctggagctcg gcgtccaacg cggcggccct
     5701 gggcggcaac gtcctctccc gtctgagcgt gccgcgcctg gccgacaggc atgcccgcaa
     5761 cctgtggggc atggggctgg gcgacttcgc ccggcagcgc agccggggag gcgtggacgg
     5821 ctacgactac aacggaggcg gctactccgt gggggcggac agcggcatgg gaggggagga
     5881 cgagggcatc tgggggatcg ccttcgggca gctctgcggc catgccagaa gccgggactt
     5941 ccaggggcgc aacacgcagg acacccggat gggctccctg tactggggcc gcctgatgga
     6001 agaaagcaac agggcgtact ggatcttcaa gggaagcctc acgtgggcgg aaacgcgcaa
     6061 caacatgacc agccgcctga gcggggcgcc ggcctccagg ggcaaatgga acaacgagac
     6121 gtggctggcg caggcggaag tgtcccggac ggcggactgt gcgggagggt ggaggctgac
     6181 gccgttcgtg aggatggaat tcacgcacgg ccgtcaggac gccttccggg aacagggagg
     6241 gtatggccgt gacttcggag gggcggcgct gaaacgcctg tccattcccg tgggcctgga
     6301 aataggcagg acggacgaat ggaaggggag gccgtgggcg cagtcgctgc gcgtgtccta
     6361 tgtgggggac gtgctgaggg acgtgccgga agcggcggtg tacagcccgt acagcgacat
     6421 gggctggaga ggaagggcgg tgagcccgga acggcacggc ctgcgcgcgg agtacaacac
     6481 gttcctgcag tgcaatgagc gctggagcgt gtacgggggc tacgggctgg aagtgagggg
     6541 aagctcctgc taccaccgcg tgaacgcagg ggtgtccagg agcttctaaa agaaagcttc
     6601 aggaaacgga atgaaaaata atgatatgat tattctatga atctgcgcaa aaaactggca
     6661 tttgccgtca tggccctggc cattccttca tcatgggcgg atatcccgga atcctccgta
     6721 tcctccgcgg ccgtctttgc cgttaaaaaa gcctggccca gggccggctt cattgaatgg
     6781 gagctcagcg gatcaggaaa actttatgaa gctgaattca acatcaacgg ttttgaattt
     6841 gacgtcaaag ttgaccctga aggaaagata ctccgggtca aggaggaaat agcggtatcg
     6901 gcccttccgg ggccggtccg cgcggccgct ctgaaaccat atccgggcgg cagaatcaaa
     6961 gaagcggaaa aagtgacgga aggaggcgtc atccgttaca aggtggaagt ggtggatcat
     7021 ctggatgatt acgatgtcct gctgaccccg gagggcgata ttctttacgt ggaccattaa
     7081 acggcttgaa ccgtctacag ccggaaacgg gcgccgccct gtcaggtaaa aaccctggca
     7141 gggcggcgtt cttccttttc ggaatatcca gtcgtcccgc ggggctggat gatgcctgtt
     7201 ttttcattgc cccccggcgc ggcgcatgcg ctcaagcgga ggtgagggcc aggcggcccg
     7261 gacagttccg gattgagcgt cccggggatg tttcctcctc cgtacggcgc gggattattc
     7321 cctgccgcgg gcgttcagga aaatgaacac gtaatacagc agcatggcca gggaggaaag
     7381 cgctcctacc acgtaagtca tggcggccca gaacagggcg ctcttggcct ttccatggtc
     7441 gaaatagttc atcaggtggg agcggtccag ccatttcagg gccctggcgg aggcgtcaaa
     7501 ttccacggga agcgtgatga acgcgaaggc tgtggtcacg gcaaacaggc cgatgcccag
     7561 ccccagcacc cacggagagc cgcccatggc cagcagcaca atgccgatca tcagcacaat
     7621 gcccaccagg ttggaggaga gctggacgat gggaaccagc gcggaccgca agcccagcca
     7681 gtggtaagcc tgggcgtgct gcacggcatg ccctacttca tgggcggcga cggcagcggc
     7741 ggcaatgctg ttggaattgt aaacggactc gctcaggttg acggttttgt ccgccggatt
     7801 gtaatggtcc gtcagatggc cgggcgtgga aatcaccttc acgtccgtaa tatggttgtc
     7861 cttcagcatc tgctccgcca cctgcctccc tgttacggga atggaaatct gggagtattc
     7921 gctgaagcgg ctcttcatgc gcgcactgac cagccagctc agaagcatgg agcccagcag
     7981 aatcaaccag taaatggtat aggaatgcgt tggggcggtt tccgccgtct gtaccgtata
     8041 agaaaggaga tcgatattga ataactcaat cattgcaaat ttgaccttat atcatcaggc
     8101 tttgttgaat ccagagggtg ccgtgtcagc ctccggaggg agtcccccgg cttctgtcat
     8161 acggaaatgt gcggaaaaac ggagaattca atcatccgtt ttttgggaga ggagcttgcc
     8221 gtccggcgaa taataaagct ccactttgcg cacggcgggg gattcggacc ggcagtccac
     8281 ccggtacttc aggactcccc gggcggtaat ctgcctggcg cggcggaggg cataccccgg
     8341 gaaatcgcgg gcgatggccg ccgtaacggg ggctggaaga tgctggaggg aaatatccgc
     8401 cttaaccagg ctgacagtgc cgtcgggata caatttcacc tcgtattcca gtccattgag
     8461 gtggaattcg gcttcataga catcttcgtc attgtcgaaa tcccattcaa tggcgcctgc
     8521 gttcggatac tgcttctgta cctgctcttt cacgggggcg ggaacgcggg aggccgggat
     8581 atccgccagg gcgggcaaag tgaatgccgc ggcaagagta gctgtaaggg tgcgtgaaat
     8641 attcataaat acggaataat tgtatttatt aaaaaaacaa gaatcttttg cggatgtttt
     8701 gtgacaattt agccatcaat gaaatgaatc cgggcatggc ggggtttctt gtcgccaaaa
     8761 agcggatggt ggagttgaat ggcggcatgc aacagggtat tgtctatttg cttggagcgg
     8821 gtcccggcga ccccggcctg ataaccgtcc gcgggcggga actggtggga atggcggaag
     8881 tgctggtata tgatgcgctg agttcggcgg aaatgctgaa ctgggctcct gctgcatgcg
     8941 aaaggatttt tgtgggcaag cgggcttccc gccacgccct gccgcaggag gaaatcaacg
     9001 ccctgctggt caggctgggc cgtgccggga aaaaagtggt gcgcctgaaa gggggcgacc
     9061 cctacgtctt cgggcgcggg ggggaggagg cggacgccct gcatgaagcg ggcatccctt
     9121 ttgaagttgt tccgggcgtt acttccgcca ttgcggggcc tgcctacgca ggcattcccg
     9181 tgacgcaccg cgggtactgc acgcagttta ccgtatttac gggccatgaa ggcgagaaca
     9241 agaaggcgtc ttccctggat ttgaggggaa tagccgaggc gcagggcacg aaagtcatgc
     9301 tgatgggaat gcggaaactg gcggatgtat gcgaagccct gatcgggtac gggcaggaac
     9361 cgtctgtgcc tgccgccgcc gtccagtggg ccaccacggg cattcagcgc acggttacgg
     9421 gaactgtgaa aacactgccc gccagggtgc agaaggccgg gttgggcgct cccgcagtag
     9481 ttgtcatcgg ggatgtggtg aaagaacgcg agtccctgaa ctggtttgaa aaactgcccc
     9541 tgttcggcaa gcgcatcgtg gtgacgcgca cccgtgccca ggccggggaa ttgagcgcgc
     9601 gcctgcgccg cctgggggcg gaagtgctgg agatgcctgt catacgcatt gccccgccct
     9661 ccaacaggag ggaatttgcg gaaagcgtgg tgcatgccca cacatatgac tggctggtgt
     9721 tttccagccc caacggggtg gaacgcttct tccaggcgtt ttttgccgtg tacagggaca
     9781 tccggagcat tggaggagcc aggattgcgg ccatcgggcc agggacggag gcgaagctcc
     9841 gggaatacgg gctggccgtg gatgtcatgc ctaaaaaatt tgtggcggag ggcctcgtta
     9901 aagcgttcag ggacgcacgc gaagaaatag gaaccattga acacagcaca ttcctgtggg
     9961 tgcgcggaga agaagcgcgc cgcgtgattt acgacgggtt gaatgcgctg ggcgctattg
    10021 tggacgaatg catcgcctac aaaacggaag cggaaacgga ggatgtggcg ggagcccagg
    10081 cggctttccg cgaacgcgga gcggacatcg tgacgttcac cagttcctcc acggcggaga
    10141 actttttcaa gctggggctt ccgtggccgg agggatgcag ggccgccagc atcggtccgg
    10201 taaccacggc aaccctcaag gagctggggc atgccccttc aattacagcg aaaacacacg
    10261 atatcaacgg tctggtggaa gccatcgtca aggccgccgg caaataggga aaacggcgcg
    10321 gacggcgtga aacgccgggc cgggcagatg ggaaatacaa ggcggggaac gccgtttaaa
    10381 agccattgac ggcttcccag ccgatgacct tcccgtcttc aaacaggaca ttgcaggaga
    10441 cgggcgggcg gctgcccagg ggcatcgtga cgcctatccc ggtgcctatg ccggaatccc
    10501 tgccccggaa tcctcctccg cccaggccca gggaccagcc cgtgccgctc cgcacgtaaa
    10561 tccaccgttc cgtattcttc ccgttcatcc tgcccgccat tttccgttcg ggttcccccc
    10621 atgccagcag aaccgccatg ggagacattc ctttttccag ctccccgcgc gccactttgg
    10681 cctgatgctc cttcgggagc tgttcataag ccggcatatt cttcgcaatg cggtcggacg
    10741 gtgcagggga acaggctgcc agcagcacga ggcaggaagg aagaagaacg aaagaaaaac
    10801 cggttttcat catcccgtaa atagcatggc agcggatggc aggcaaggtg gcagagcggc
    10861 ttttcggaaa aatggagatg agggaatccg ttattttgac ataaaagcgc gcttttcccg
    10921 taaatacata attggttatt gacaagcatg gggggtaggg gagtaggaat tcagctccaa
    10981 atgcaacgcc cccttccccg tgcgggcagc gtcgctttta ttgttcagca cttttttccg
    11041 agccgccgat atgtccaaaa cgttaattat agcagaaaag ccgagcgtcg ccactgattt
    11101 ggcgagagtg ctcggcaaag agcttggaaa attcacccgg gacaagtccg gggcatttta
    11161 ccagaatgac cgggccatca tcacctctgc cgtaggtcat ctgctggaac agaaaaaacc
    11221 catgacggaa gaaggaaagt ccctgccgtg gaaattcgac tatctgcctg tcatccccag
    11281 gaagtttgaa ctggaaccca tcaagcagtc ggaagaccgt ttgaaaaagg ttcttcagct
    11341 tgccaggagc aaggacgtca cggaaatcgt caacgcatgc gatgccgggc gtgaagggga
    11401 actgatcttt cacaatctgg tgcgttacgg aaaatggacc aagcctacgc ggcgcttatg
    11461 gatgcagtcc atgacggatg aggccatcct caacgcatgg catggcatgc gcagcgaagc
    11521 tgacatgaag cccctgacga atgccgccgt gtgccgttcc gaatcagact ggctggtggg
    11581 gctgaacagc acccgcgccc tgactatcct gcaatcccgc ggcggcttca acgtcacccc
    11641 cgccgggcgc gtgcagacgc ccacgctggc tattctgacg cagagggaat tggaaatcca
    11701 ggcgtttgaa ccggagccct attcggaagt atgggccgaa ttcgacgttc agtccggctc
    11761 ttactccggc cgttggattg accgtgactg gaagaaaaac gatcatcccc actcccgcgc
    11821 ggagcgcatc tgggatccgg aacaggcggc cgtcatccgc gaccgctgcc gcggaaaagc
    11881 cggcacggtg acggaagaaa agaaaccggt cacgacggtc gccccgctgt tgtatgacct
    11941 gacttccctt cagcgggaag ccgccaaccg ctacggtttt tccgccaagc gcacgctgca
    12001 gctggcccag gaatgctatg aaaagcacaa ggtgctcact tatccgcgta cggactcccg
    12061 ctacctgccg gaagattatc tgggcaaggt ttacggcatc gtaggcacgg tggcggagca
    12121 gaacccggaa ttcgccccct ttgccaggga cattttggac aacaagcgca tcaagcccaa
    12181 ccggcgcgtg tttgatacaa aaaaggtgag cgaccacttt gccatcatcc cgaccgggaa
    12241 gatggagaag cttaccgggg acgcgcagaa actctttgaa atggtcatgg cccgtttcct
    12301 ggccgtcttc ttccccgctg ccgtgtttga agacacgcgg agaaccacgc tcattaccca
    12361 ccaggacggc gtgacggacg ctttcctgac cacgggccgg gtgcttgtgg agccgggctg
    12421 gcaggccgtt tacggacgga aggccggcac ttcctccaag gaggagctgg tgcccgtccg
    12481 cgacggggag caggccgcct tgcgtgagat tgaaatccgc aacgccatga ccaagccgcc
    12541 cgcccggtac aacgaagcca cgctgctggc cgccatggag ggggcaggaa aactggtgca
    12601 tgacgaagaa ctggccgccg ccatgagcga acgcggcctg ggcactcccg ccacccgcgc
    12661 ctccatcatt gaagggctga tttcccagga atacatcgtc cgggacggcc gcgaactttt
    12721 ggcgacgcgc cgcgggattg aactgattgc cctgctggaa cgcatcggcc tgaaaacgct
    12781 cacctccccc agcctgaccg gggaatggga atacaagctc aagcagatgg aacaggccgc
    12841 cctgccgagg gattccttca tggagggcat taagaccctg accggggaaa tcgtgaagag
    12901 ggtgaaggat ttccgggaag cccagcagca ggtggaactg ccggaaatgg aattggactg
    12961 cccttcctgc catgcccacg gcctgaaaaa cacgctggac gccgtttcct gccgttcctg
    13021 caagttcagc atccgcaaag ttatctccgg ccgcgacatg acggatgaag aattgaagac
    13081 ccttattgtg gacggcagga cgggaattct ccacggattt acctcccggt tcggcaagcc
    13141 ctttgaggcg gggctggaac tcaacgacaa attccgggcc acgctttact tcccggcgcg
    13201 ggaggaagat gaagccgcag gggtggagga agccgtgaaa gtggcttccg tcgccattcc
    13261 ggacatggga gagcatgatg tgatggaaac ggccaaggcc tggaaaattc cttccctgac
    13321 ccttggaaag gaacatgccg ccgtctccgt ttcccggact atcctgggac gtgaaatccc
    13381 gctggaccag gtattgaaga ttttggcgga aggaaaatcc gatctgatga aggggttcat
    13441 ttcgcagcgt accaagcgcc cgtttgacgc ttatctggtc ttcaatgcaa agacggggaa
    13501 aataggtttt gaattcccgc cgcgggaaag gaaatatccc gctaaaaacg ctgccggaaa
    13561 aacggaaggc aaggcggaga agacgggccg gaacgccgcc aaaaccgccg ggaaagactc
    13621 caaatccggg ggggctgcca aaaaggggcg caagcaataa cggcagcgga aacattccgc
    13681 cgggaacgaa gattgggaaa agagccggga aaaagcttct ttccggctgt tcccgtatgt
    13741 tagcttgaaa gcgcacccct ggattcctga cgatgaaatg ccttttattt gctgtgctgg
    13801 tgtggtgcgg cgcggtttgt gcgccggcgg aggaatatgc ctttccgggg gaacgggaat
    13861 ggatttccaa atttggagat gtggtgaagg gcagattcca ttcctgcaca ggggccgggg
    13921 cagttctgaa aatagggcga aggcatgtgg aaattccgtt aacccgtctg gatgaggaag
    13981 acgccgcgct tgcggtttct ctgacgggag cggaagcgcg ccggaacctg aaactggccg
    14041 cgatgctttg gcctttcctg atgaaggaac tgcagaaagt gacgtggacg ccggcagaaa
    14101 agaatatctc gccccggtat catgaaatgg cccggaagaa gaatgccgga gctttaacgg
    14161 gaaccagaaa agtggatgga ctggtttgcg ccacatcgct ttccatcatt caggggcctc
    14221 cgttccgtat ccgggaatac ttccgtccgc agacgcgtcc ggaaagcggc aggggacaga
    14281 agaatttcga tgtggttctg gagcagggga gccgctgcgc ttattatgat atggataagg
    14341 gtgttcttat tccctccaaa tgcctgtctg aggaacggac ggaaatgcgc gctcccgttt
    14401 cctctctgct ccctccggca gaacagcttg ccgcgagaga atcaaaaaat tacaggaatg
    14461 cggattactc cctgcttgtg gaaaaccgcc ccgtcaaggg agcctatgaa acgtgcaagc
    14521 ccaattccct gggaagaaaa aagctggtca tgtactggaa tacggagggt gaaannnnnn
    14581 nnnnggtgaa atggtttgcg gcgtcatgaa gctggactac gacaacatgc gcgtggccgc
    14641 attattcgat ggacggcgga agctgatgaa cgcccctggg cttggatatg ccgtgattct
    14701 ttcccggctg gataataaag gcagggactt gaaggagaaa ggccgttact accatgtggc
    14761 gttggctgca gacggcctgc ccgtgtatta tcatgagggc ccggaagatg aggccgccat
    14821 gaacccgctg ctttacggga aactacgcga ttccgagaaa aaggcggctc tggcggagga
    14881 gaaaaaacgt gaacaggcat tccgggccag gcgggaagcc cggcggcgcc tggtggaatc
    14941 cgccaggaag cgtaatacct gaaaatagtt cctgtctttt cgcgacgggt atccttcttt
    15001 tctggaaagc gttcgtgaat gttcttctca ggagcgctgc cctggcgtat tgccgaattt
    15061 tccagtcttt cagaaaggct gattatttat tcctgccggg gaatttttca gaactatccg
    15121 aaataattaa aaaacgctgt tgttatccgt tatctgagag ttccacggtg cggaacaaac
    15181 ccatcacacg gattcggttg gctctttttc cagcggtttg tgatcttcta tcaatctttt
    15241 gatatcttgt cttgtttttt tatctctcga ttttcctagc tttctgtaaa tatcggtcat
    15301 gtcaaagtag attaattttt cctgcccctg atcatttcta atagaaataa tctgaatata
    15361 tttgccatca tgcatggtat atatgtaccc tagtatgcca tatccctcat agtgtttcct
    15421 gatatattcc ttctgagatt ccctgatatc gtctgttttc ttgattcctg taagaataat
    15481 aggattgatg attgaatttt ccctgtcaat tttctggctg ccgggaatta catcttccga
    15541 accggcggca ttattaatgc aaaaacaggg gaagcaggct atcgacaaaa aagcaatttt
    15601 cagtaatggt ttcatatcag tttatttatt aatcagtttt atttatttat gttgtcattg
    15661 cagccgacgc aggtgcagcc tgcttcgttg cagttgccgc attgcaaacc gcattcttct
    15721 gtcaaacgca gccgggccat tgcgatgact gtgctttctc cttctgcata tgcctcggct
    15781 ttgccctctt tttcatcatc tgtaccggtt actaccgggg taattgtctt tcgcagcggt
    15841 attggccccc ctgtctcacc cgtgtgatga acgcttgtat acttacagga tatatagact
    15901 gtgtaagtgc gtttggcgtt ggctaccctg actggctcta tgtttaaatt ttttaattcg
    15961 tattcacatt ttaattcctt tgcattccca taattgcaaa taacaaaggt caaaatgcct
    16021 aataactgca atatcttttt catcctaaaa ttatgggaca attttgatat atgtaaacat
    16081 atttttaata tgttttacat aatgattgct tgatagatag ttacaaaaat atctaacaaa
    16141 ataataatta tccgggaaag accccgggga ggatgaggcg ctgaatagga gtgtccggcg
    16201 ttccaacgga gaagcaacgc cttcgcatct cgggagaagt tgaaaatgcc cggattggct
    16261 ttaattccgc ccgaaggcgc ggattttacc gggggcatgc cgccatgctg cggcgttcag
    16321 caaaggcaca gttgagctgc gggcccttta tatttatccg ccatacggga taaaatggga
    16381 gattctggta tgccgagggg ccgggattcc actaaaatgc catgtttttg gcatgaggag
    16441 gctgttttct ctggagcctt tcagtttgag ttcctgctgg ttttccatga acatggtgcc
    16501 ggattgttcc ctcaatgctt ccatatggcg ggtaagaagc tgctggcttc gatggtccgc
    16561 atctgggcat cctgtgaaag cgcttcatgg ttttcaagtt tccctgttga tgttgagtct
    16621 cccgcagtct ttttcgatcc atccggagag gatgggaccg gtgaatcgga ttcaggtcaa
    16681 ataacctgtt ccggggtggt tcaggaggca tcaaaagctt tcagcactat ttcttattcc
    16741 cggtccgtgt cccggatttt gatgttgatg aagattattc ccggacgggt gttatttgcc
    16801 gtcttttacg gccgtgtaca ttagccgtat caatggaggc ggatcctgct ttccgcgagc
    16861 cggagatgcg ctgaattccc ggtgttcagc caatgcatat tttcagggaa agaagtgatt
    16921 cctgttcttt cctgaaacat ttcctgcctt ctttatgccg gaagcggcaa aggggcgtgc
    16981 ggcatatgcg gcagtattat cttcctgcct ggaggcagcg gtagtaataa tccacgcggg
    17041 aaatgaacca gctccatttg tacccggggc gcccattagt catcagcggg gcggggcaat
    17101 tcttgccgga ccagtagttg tgaggcacta cgttgcgcag ggggacgttg tagcgcatca
    17161 tcagcagcgc agccagcttg gccgtcttgt tgtagatgac gacagggttg tgcgtggtgc
    17221 attcacacat ttcaatggct acggagtatt ggtctcccgg tccggaatgg tccgcatggt
    17281 ggccggtttc cgtagtgggg atgtgctggt aggcgccgaa ttggtccaca gtgaagtgcc
    17341 agttcaggct gcgggacttg ccgctgttca ggtaacggga gtgggcattg gcgtctcctt
    17401 tggggttggc ggtgctgtgg atggtgatga agcgggggcg catggaggat gcgcgcttgc
    17461 gggcatacgt gtttttgggc atgtaggaaa ctttaatgcc tacttcacgg cccatctggc
    17521 cgggtgtgcg gggaactacc ggcgcctttt gaattttaaa ggagacggcc ggcggcctca
    17581 ttttgtcgtt ctgcgaacac ggcaaaaaag cccgtccaga caaatctata ttccatgcgt
    17641 ttgttctagg aacagcctgg aaggattgca agaagaattt ggaattagtt cggatgaact
    17701 aatcaggccg tgccgtgaag ttgcaggtat atccggtgcg gatgaggcgg acgggccgcg
    17761 ccttcgggag gaaggggccc gtccgcgagg ggaagccgta ccgggaacgc ccggtacata
    17821 tcccgttatc tgaggatttg ggtaaggaca tattgcagga tgccgcccgc cagatagtat
    17881 tctttttcaa taggcgtatc aatgcggacg atgacaggca cgtcaaatgc ttccgcatcc
    17941 gcgggctgta cgcgcagggt gacctgctgg cgcggagcga tgccgttgtt caggcccagg
    18001 atggagaagg tggcgtcctt caacggagtg atgcggtcat aatccgcctt gtcggcgaag
    18061 ttgaggggca ggacgcccat gccgataagg ttggagcggt ggatacgctc aaaactcttg
    18121 gtgatgacgg ctttgacgcc cagcaaattc gtgcccttgg ccgcccagtc gcgggaagag
    18181 cccatgccgt agtcttctcc gccgatgacg atgagcggaa cgccgtcctg agcgtaggcc
    18241 atggccgcat catagatgaa ggcgggcgtt cccgcggcag gggttatttc cttgtccggg
    18301 gcgggaacgg cgcggttgcc gaaatacagt gtgtaaccgc cttccgtgcc gtttaccatc
    18361 aggtttttaa tgcggacatt ggcgaaggtg ccgcgggtca tcacgcggtc gtttccgcgg
    18421 cgggagccga aggagttgaa atcttttctg tccactccca tgccctgcag gtacagccct
    18481 gcgggccctg tttcccggat ggctccggca ggggagatgt ggtccgtggt tacggaatcg
    18541 ccgaaaatgc ccagggggcg ggcatccgtg atatccgcca gtgtgcgggg ctggccgttg
    18601 aagccgctga agaaaggagg gtcctgaatg taggtggatt gttcattcca ctggaagaca
    18661 gagccttccg gcgcgtatac ggccgcccag cggggattct tgtccggcgt gatggtgtaa
    18721 agctcccggt atgcttccgg gttgcgggat ttggcaacgg cttccgcaat ttccccggag
    18781 ctgggccaga tgtccttcag aaatacgggg ttcccgctgc ggtccgtgcc cagcggttcc
    18841 gtctggaagt cgatgtccac gcggccggcc agcgcaaagg cgacgaccag gggaggggac
    18901 atcaggaagt tggtcttgat ggaggcatgg atgcgggctt caaagttgcg gttgccggac
    18961 aggacggaag ccgctacaat gtcattgtcc cggacggcct gttccagttc cggatttaac
    19021 ggcccggagt tgccgatgca ggtggtgcag ccgtagccga cggtttcaaa gcccagagcg
    19081 tccagggatt cctggaggct gttggttgca aagtaatcgg aggcaatacg ggaacccgga
    19141 gcaatggacg ttttcacgtg cggaggcacg ttcagccccc gttcaaccgc ttttttggcg
    19201 agcagcccgg cggcaagcat cagccccggg ttggatgtgt tggtgcagga ggtgatggcg
    19261 gcgatgagga tgctgccgtc tttcagcttc gcttcctggc tgaagccgtc caccggaacg
    19321 gggacgcccg ccggggagtg catggaaacc gtgacctccg gagattcctt cttgccgtaa
    19381 cctccttccg ccacgggcat gctgacaagc ttgcggaagc tgtccctgat ggagttcagg
    19441 cgaatgtggt cctgcgggcg tctggggccg gagacggctg gttccacggc agacaggtcc
    19501 agttccagct ggtccgtgta gtccagatct ccgttacggg gcattcccca caggttctgt
    19561 gccttgaagt agttttcgta cgtagtgatg gcttcttcgc tcctgcctgt ctggcgcaga
    19621 tagtcggagc agcgttcatc catggggaag aagcccatgg tggcgccgta ttccggggcc
    19681 atgtttgcta cggtagcccg gtctgccaga ggcagggccg ccgccccgtc tccgaagaat
    19741 tccacaaatt tgcctacgac gccgtggctg cgcagcatct gggtgacatg cagcgccagg
    19801 tccgtggcgg tcactccttc gcggagttct cccgtcatat ggacgcccac tacttccggc
    19861 accaggaacg tgacgggctg gccgagcatc ccggcttccg cctcaatgcc gccaacaccc
    19921 cagcccacaa cgcctatgcc gttaatcatc gtggtatggg aatccgttcc caccagggta
    19981 tccgggaagt aaacgccatc cttttccatc acgccctgcg ccaggtattc cagattgacc
    20041 tggtggacaa tgcccacgga cggaggaacc acggaaaaag tctgaaaggc ctgctggccc
    20101 catttgagga attcgtagcg ttccgcattg cgctggaatt caatgtccag gttcttttcc
    20161 agagcatccg tgcatcctgc ccagtccacc tggacggagt ggtccaccac caggtccacg
    20221 gggaccagag gttcaatgac ggaggcgtct tttcccaatc ctgctacggc ggagcgcatg
    20281 gccgccagat ccaccagaag agggacgcct gtcaggtcct gcaggacaat gcgggctacc
    20341 gtaaacggga tttcatagga accgggattg ttggcattcc aggaagccag gttgtccacg
    20401 tccttttcag tcactttcag gccgtcgcaa ttgcggagca gggattccag aacgatccgg
    20461 atggaaacgg gaaggcggtt caggcgggga tagccgtttt ccgccaaggc tggcagtgaa
    20521 taaaactgtc ccggggttcc ggctcccgtg atgaacgtac tcttggcaat cttggtcatg
    20581 atgcggaata gagggttaac tgtaaacagg cggctgccct cctggcagtc gcgggtacta
    20641 tacaggccaa atgtgataag gcaaggactc tgtgtgacgg agggcggatt tttccccccc
    20701 ttccgaagcc ggggaaattt tgctctgaac cggggcggcc gctttttccg gcattttttc
    20761 tccatgcacg ccgcaggaat catggagtgc gtcatggaac attgaaaatg agttcgcagt
    20821 attgtccaac cctgtgaaag gtttcaagat gatgaacgca aaaaacatct tatcaatcat
    20881 agcgggagcg ttggcgctgg ctgcctgttc tggcccgcag gaagagaatt tgcgcatggt
    20941 gcccctgacc gtgcctgcaa ccgtacccat gacctcctac ggaccgcacg cgagggtggt
    21001 tgtagcagga attgattata cggaggtgca cgacgaatgg atgaatcccg atatcatgaa
    21061 aaaggctacc ccgggcaata cgcgcgtggt gatcagccgc ggtcgccaaa ggggccagtt
    21121 gatggtgggg gatgaggtag ccatggattt tcccgtttgc gtgggtaagg cttcccaccg
    21181 cacgcccgtg ggccatttcc gcattacgga aaaggcagtg catcatgttt ccaatttgta
    21241 tgatgcctcc atgccttact tcatgcggct gacggacagc ggcattggga tgcacgtagg
    21301 ccctgttttc cagacgccgc agtcccatgg gtgcattcgc atgacccgtt cttcctgcgt
    21361 gccgcttttc aaaaccgtta aggtaggcac gcccgttacg attgtacagt aatgcccgga
    21421 aaatttccgg gcgcttttcc ggaagaaaaa ttccccgcat ttttttaaaa ctcatttttt
    21481 taaacaaaaa aacgtcatcc gccgctattt taataaacgg catgtagata aacggtactt
    21541 gccgcaagtc gcattgcctc agcaacccga accctccatg ccgctgccga ctgtatcgta
    21601 ttcattcatc ggcagcggca tggagggaag ccgtctgctt gaccgcagaa cttggggaat
    21661 gaacgggctg cccgtatttc gtgaagagga catggtgctt gttctcccgg gggcgcattt
    21721 gttcctccgt gttgtaaaag attaatggag aagaaggatt ggcctcccct gctttttctc
    21781 cggattattt ttccgcagct ttcctgtctg ttccttcccg gtgcagcagg ggaaggtatt
    21841 ttccataacc ctgtgcttcc atgtcctgtt caggaatgaa tttcagggag gcgctgttga
    21901 tgcagtagcg cagtccgccc tgttccttcg ggccgtctgt gaaaacgtgg cccaggtggg
    21961 cgtctcccgt tttgctgcgg acttccgtgc gcctcatgcc gtgggagaaa tcctgttttt
    22021 cctgaatcag ttcttctttg atggggcggg agaagctggg ccagccgcag cctgaatcaa
    22081 acttgtctgt ggacaggaat aaaggttcgc ctgtcgtgac gtccacataa atgccgggtc
    22141 tgtcgttatt gaaatattca ttgcggaagg gaggttccgt cgcattattc cgcgtgacgg
    22201 caaattgctc gcgtgtcagg acgcggcgca gctcttcgtc cgattttttc cggtaaacag
    22261 gttcgttttc gcgggcttgg gacgcttccc ggaatttggc gggtggaata tggcagtatc
    22321 cgccagggtt tttatccaga tagcgctgat gatatttttc cgcccgtgaa aattggcgga
    22381 ggggctgaac ttcaattgcc agaggctgcc tgaagtgctg ctggagcctt ttcagggacg
    22441 tttctataac aggtttgtcc ttctcatccg cgtaataaat gcccgtgcgg tattgaattc
    22501 ccctgtcgtt tccctgtttg ttgacggaaa caggatcaat aatggagtaa taccgttcca
    22561 gaagaaaggg cagactgagt tcttccgggt caaatactac ttttaccgtt tccgcatggc
    22621 ccgttttccc gctgcatact tcttcatacg tgggagaagc cgtggagccg ttggcatatc
    22681 ccgcttccgt ttccttaacg ccgggcatca gggagaagta tttttccact ccccagaaac
    22741 agcctcccgc caggtaaatc gtttttaaat ccttgttgtc catatgaggt ttgtcattac
    22801 tgcttgcctg gcctttttcc ccgcaggaaa aaaaggagag catggcgact gcggccaaaa
    22861 tggcgcatgc cagtccggcc agtgtccatg gtccgtggta aatacgcttc ataaacgctg
    22921 tatggagtcg ccttccggtg cgggagcagc ggctccctgg gccggggaac tgctgtcccc
    22981 cggcttttcc ggaaaggtaa atgattagac aacagcgggt tcattccgtt cgatacctgt
    23041 gtccatattt ccttatttct cccgtttttc cgtcttcatc ctgtttttca gccactggta
    23101 aatagcccgg tcggaggcat ccggttttct gcttgtctgc atgctgaatc cgtatttggt
    23161 tcggaagttg aaaacggcca gagaagggct caggccgatg gaaagcccct ccatccgtat
    23221 ttccacctct tgcgggacat ggctggagaa gtcgaggcgt ccggcattgg tgacggtttc
    23281 cacatagtgt ttcaggaaag gctggatgct ggccaggaag gagggttcca gagccatgcg
    23341 gcagcgttcc ggggaagaat tttccctgac aaaaatgacg gaaggagctg tgatggtatg
    23401 ggcgggcctt aacgtattca gcggaacatt ccataaaaag gaaaacagcc ccgtgcccag
    23461 gagaaaaaga aggacggcca gggcgccaag ccatttgacc cgtttgttta ctgattgatg
    23521 acagggccgg ccttcgttca tggagagaag catggcataa tattccatgg tgatccatga
    23581 aagaatttgc caggaagcag atgggaattc ccgtccccgc atgagggaat ttttgtacta
    23641 taacatgaaa agcatgaaaa aaacgctgga aatcatgtag agtatcatta aat
//
LOCUS       DAWEMX010000026        23105 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_919_length_23105_cov_11.417744, whole genome shotgun sequence.
ACCESSION   DAWEMX010000026 DAWEMX010000000
VERSION     DAWEMX010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 23105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23105
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_23105_cov_11.417744"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2434..2443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4403..4412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5403..5412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11524..11533
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17452..17461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18022..18031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5412 a         7264 c         5823 g         4546 t
ORIGIN      
        1 gggctcaatt tccaaacgca ccttgatata aattatttga attttacaac acgggccatt
       61 gaaaaaagat aaaaacgctc tccatgttcc tcctcccgcc tcacgtcatt ccgagtgaac
      121 acgcttgcag aagaggtaat ttggtagtag aattttcgcc atgttattgc agcctgccga
      181 acgccgagat ttcatccgcg atatgatttc tgatgacctt gcctccggca agcatcaggc
      241 cccggtaaca cgctttcccc cggaacccaa tgggtatctt catatcggac atgccaaatc
      301 aatctgcctc aacttcggca tcgcccagga atttccgggt gcgcgctgcc acctgcgttt
      361 tgacgacacc aaccccagca aggaagacca ggaatatgtg gacagcattc aggaagacat
      421 ccgctggctg ggctttgact ggggcgacaa ccttttcttt gccagcaaca tgttcgactt
      481 cttttacgaa tgcgccgttt ccctcatccg caagggattg gcctacgtag acgaacagac
      541 ggtggaggaa atacgcgccc agcgcggcaa cgtgaacgtg cccgggcagg aatcacccta
      601 ccgcaaccgc tccgtggaag aaaacctggc acgcttccag gccatgaaaa acggggaaat
      661 cccggaaggc agagccatcc tgcgggccag gatagacatg gcctccagca acatgaacat
      721 gcgggacccc gtgctgtacc gcatcatgtt cgcggaacac cacaacacgg gcagttcctg
      781 gtgcatttac cccatgtatg acttcgccca cccgctggaa gatgcctacg aacacatcac
      841 ccattccctc tgcacgctgg aatttgaaaa ccaccgcccc ctctatgact gggtcattga
      901 aaactgcccc gtcccggcac gcccgcgcca gacggaattc gcccgcctga accttaccta
      961 caccgtcatg agcaagcgca agctgctgca actggtgcag gagggacacg tctccggctg
     1021 ggacgatcca cggatgccca ccgtctccgg catgcgccgc cggggctaca cacctgccgc
     1081 catccgcaac ttctgccaca ccataggcat cactaaattc aacggcttca cggatgtggc
     1141 cctgctggaa tacagcgtaa gggaagacct gaatgccaac gcgccgcgcc gcatggccgt
     1201 actcaatccc ctcaaggtca ctatcaccac cctgccggaa aacgcggaag aaatggctga
     1261 agtgctgaac aatccggaaa agccggaaga gggcgtccgc cgcctcccca tcacaaggga
     1321 agtctggatt gaacgggacg acttcatgct ggaccctcct aaaaaatact tccgtctggc
     1381 ccccggccgc accgtacgcc tccggggggg ctactgcatc acctgcacgg actacaagca
     1441 ggatgcggag ggaactatca cggaaatctt ctgcgaacac attcccggca ccatcggctc
     1501 caaccctccg gaaggaatac agtgccgcgc cgccattcac tgggtaagca ccaaatacgc
     1561 ggtggacgga gaaatccgca tctatgaccg cctttttacg gaagaaaacc cggacgctgc
     1621 tgaagaaggg ttcctgtccg tcatgaatcc ctcttcgctg gccatcatca caaacgccaa
     1681 gctggaaccc tccctggccg gagcggaacc agaattccgc tgccagttcg aacgtctcgg
     1741 ctactttgtg gcggaccgca gggaccatgt accgggccgt cgtcctgttt tcaaccgcac
     1801 ggtggctctc aaggactcct gggcaaaaaa acaaaaataa cctctcttca tcccctttta
     1861 tggacaatca acttcaattc tgcaccgttg aagaagccgt ggaagaaata cggcagggac
     1921 ggatgatcat cgttacggac gaccctgggc gtgaaaatga ggcagacctg atcattgccg
     1981 ccgaattcgc tacgccggaa gccatcaact tcatggttac ccacgccagg ggcctcgtct
     2041 gcgcacctct ttcgccggaa cgggcggacg ccctccaact cccgctcatg acctccgtca
     2101 accgggaaaa catgtccaca gcattcaccg tctccgtgga tgcggcccat gacatcacta
     2161 cgggcatcag cgccgcggaa cggtccctca ccattcgcac gctggcggat cccaaagcca
     2221 ccgtcaacga ctttgttcag ccgggtcata cattccctct ccgcgccgtt cccggcggag
     2281 tgttgcgccg cgccggccat acggaagcca ccattgacct cgtgcgcatg gcaggccttc
     2341 agcccgccgg cgtttgctgt gaaatcatga aggaagacgg caccatggcc cgcatcgggg
     2401 atctggggga attccagaaa aaacatggcc tgannnnnnn nnngcttatt gaataccgcc
     2461 gggccaagga aaagcaaatc cggctggtgg aaaccgtcaa catgcccaca gactacggag
     2521 actttacctg ccgcctttat gaatcccagc tggacggagc tcttcatctg gccctggttc
     2581 acggggaaat ctccccggac aagcccacgc tggtgcgcgt gcatagcgaa tgcctgacgg
     2641 gagatgtctt cggctcccgc cgctgtgact gcggcagcca gctgcacacc gccatgcgcc
     2701 gcatcgccca ggaaggaggc gtcctgctct acctgcggca ggagggccgc ggcatcggac
     2761 tggcggcaaa actcaaggcc tacaaacttc aggaacaggg tctggatacc gtggaagcca
     2821 acctcaggct cggctatccg gacgacctgc gcgactatgg catcggagcg caaatcctgc
     2881 atgacctggg agcaaaccag ttgcgcctgc ttaccaacaa cccgcggaaa atcgtggggc
     2941 tggagggctt tggcataaaa atcacggaac aggttcctct catcatccca cccaacgaac
     3001 aaaacagcaa atacctggct accaaaaaat gcaagctggg ccacatcctg taacattcca
     3061 ctccatccct tcatgtccac ggaacttccc cgccgccagc gcgccacggg cacgcgcgcc
     3121 aaaatctgca tcgtcgcctc ggaatacaac gaacaataca cccaggccct ggtagaaaac
     3181 tgctcggaag aactgaaaac cgtgcttccc tcggtccgcc tggaaatcat ccgcgtgccg
     3241 ggagcctttg aaattccggt ggccattaaa tccgttctct cccgctctcc ggaaaaacgt
     3301 cctgatgccg tggtggctct gggggtcatc ctgcgcggca gcacagacca cgcggacctt
     3361 atcggctcca ccattaccca ggccctgatg cagctcgccc tggaattcac cacccccgtc
     3421 attcatgaag ttctgctgct gaatgatgaa aagcaggcct tcgcccgctg tatcgcctct
     3481 cagctcaacc gcggccgtga agcggcccgc accgctgccc gcatggcgga actcttcctc
     3541 aactccctgc tccggcaata atacctatta ctctctcctc tctatcgcca tgctctcacg
     3601 caaccaaatc agacaagccg ccctccaata cctttacgcc gcctctcaga cgccggaaac
     3661 ggaacgggag ggaatctggg acatcctgat ggaacctttc cggggcgact actgcaagct
     3721 caaggccaag gccgtctccg gccaccttac acgggactat ccggacaagc tgcgcctctt
     3781 catcacccgt gcgcgggaaa ctgcggacaa acttcagcaa gaccccctta ccctgcctgt
     3841 ccgtgaccag cttcaggacc tgctggccaa ggaaggagaa ttcaacgcct ccctgctccg
     3901 gctgaaaaaa gccctgcacg aggatccctc caatgacaag gggtcccttt ccgccgcctg
     3961 cgacgccgct cagggactca ataccgcact gatgcagatg cgccgccgcc tgctggacac
     4021 gctcaaggac ttccctgcct ataacgcaat ctggccctcc ctcatttcct cctgcagcaa
     4081 actccaggaa atcaatgacc gcatcaactg cctcatccac ccggacgacc gcccctccct
     4141 ggcggaaata aaaaaagtgg tggaggcagg aagggatgcg gaggaactct accgggaagc
     4201 caaaaccctg ggagaagaca tcctgcgccg ccgggacggg ctggacgccg ccatcgactc
     4261 caccctggaa aactactccc ctgaacgtgt cagcgccatt gaccgcgcca ttctgcgcct
     4321 gggggcctat gaactcatcc atcgcaaaga cctcccggcc cccatcgtca tctcggaggc
     4381 catccgcctg tcggaacgct tcnnnnnnnn nnccggcata actccgcttc gcaccatggc
     4441 aggattcttt aaaaaactat tcaccaaatt ttcccgaagc tcccggatag actgggaaga
     4501 actggaagct gagctggtag ccgcggacat cggcatcagg cgcgctatgg gtattgcgga
     4561 ccagttgaag gaaagcaaag gcctggatgc ggaaaatctg gtggaatcca cccgtgaagt
     4621 gcttcgccgg gcctttcctt ccaccgctcc gtccctgccc gcccctccgg aaggcaaacc
     4681 gctggtcatt ctggtcgtgg gcgtcaacgg cactgggaaa accacttctg cggccaaatt
     4741 ggcccatttg ctgcaaaaac agggctcccg cgtccttctg gctgcggcgg acacttttcg
     4801 agcggccgcc gtggaacagc ttcaaagctg ggcggacaag ctgaacatcc ccatctgcaa
     4861 gggagccccc agccaggatc cggcctccgt ctgctatgag gcccatacgc aggccatcag
     4921 ggaaggcttc caatacctca tttgcgatac cgcggggcgc ctccatacgc gccacaacct
     4981 catggaggaa ctctccaaaa ttcgccggac tctcgccaag caggatgcat ccgcccccca
     5041 cagcaccctg ctggtggtgg atgccaccac aggcgctaac gccctggcac aagccaggga
     5101 atttcataaa gcgacgccct tggattccgt cattatcacc aaaatggacg gttccggaaa
     5161 gggaggcgtg gccgtagcca tcatggatga aatgcacatt tccccctcct ttctgggaac
     5221 cggggaagga gccgaagact ttgaaccctt caaccgggac cgctacgtgg attccctgct
     5281 gtaacgggac cgcctcaaat ccatgccacc tcttcccctc atcactgccc agacggagga
     5341 aaagctgctc gccttcctga cggaacacgg gcacaccaaa ttccgcaccc agcaggttct
     5401 ggnnnnnnnn nntggcgcaa gcgcgtcacc acctttgacg cgatgagcaa cctccctccc
     5461 gcgctcaaaa atctgctggc ggaaaatttc cgtttccaca cgccggaaat cgtggaaatc
     5521 cacggttctg cggacaccac acggaaattc ctcaccaaaa tggaagacgg cagtctggta
     5581 gaatccgtca tcatccctgc cgcagcagcg gaaaatggag aaaaatcgga gcgcgtcacc
     5641 ctttgcgttt cttcccaggt aggctgcgcc ttcggctgca aattctgcgc ttccgggctg
     5701 ctgggcctta aacgccacct ctccacgggg gaaatcatcg ggcagattct ctccgcggaa
     5761 gccattgcgg gaaaacgggt caacaacatc gtcttcatgg gcatggggga acccctctcc
     5821 aattttgaca acctggcgga tgccctggaa atcatcactt cccaccgcgg tctggaaata
     5881 ggggcgcgcc acatcaccat ctccacgtcg ggattcgttc ccggcctgaa aaaactggcg
     5941 gcctatccca ggcaaatccg tctggccgtt tccctgcacg gagctacgga cgaagttcgc
     6001 gaccaaatca tgccggtcaa caaaaaatgg ccgctctccc agctcattcc ggcccttgag
     6061 gaatggaaca ggggaagaaa ccagatgccc acgctggaat acattctcat cagggacatc
     6121 aacgattctc caaaagacgc gtcccatctc gtccggatcg ccaaacgcct gcacgccaaa
     6181 gtcaatctca tcccgtacaa caccgtggag gggctcccgt ggaaacgccc ttcggaagaa
     6241 cgctgccgtt ccttccggga tgcagttcac aaggcgcgca tccccgttac catgcggtat
     6301 gaaaaaggcc atgacatcaa cgccgcctgc ggccagttga ggctgagaaa ggaacaggaa
     6361 aagagcggaa agacgtcccg ttgacggaac ggagcttttc cgaatttaca acgttgccta
     6421 ttgcccggcg ccctccgttg tgctatcctt tccggccgga tgccacccat ccagccgcct
     6481 caccatggaa accacgacaa ccaccactgc cgttcccccg ctggaactga ccagggcacc
     6541 cctcaaaacc ccgtccctgg acctcagaga cattccgcag gctcccggcc cggaaaaagg
     6601 agtgctggcc ctcatggcca tggacccggc cacttacgtg gggcagtgcg tgaccatcgg
     6661 catgacggag gactacttct acctccccgc gcacaagctg ctctggcgcc ttttccaggc
     6721 ccgctataac aaaaacgaac ccattgacat cgtttccatc acccaggctt tggaagacat
     6781 gcaccagctg gaagccgtgg gcggaagcgc gggcctggcg gaaatataca cctttaccac
     6841 taccggagcc tattttgagc attacctcaa tgtcctgaag gacaaattca tccttcgctc
     6901 catcattgac atagccaacc agtccaccac gcaggccttt gacaatccgg acgacgtagc
     6961 ggaactgctg gattctgtgg aaacccatat tttccagata cgggaacgct ataacagcgc
     7021 cagggatgaa caaagcctgg ccagcatcct taaacaagcc gtcaccaatt ttgaaaaatt
     7081 catcgcaagc aaaggccaaa tccagggact gaccacggga tttgaggaac tggacaaaaa
     7141 aagcaacggg cttaaaccgg gggacatgtt catcatcgcc gcccgcccct ccatgggtaa
     7201 aacctccttt ctgctcaaca tcattgagca catcgcgctt aacgaaaaaa agcctactct
     7261 gctcttttcc tgtgaaatgc ccgcagtcca aattgtggaa cgccttcttt ttgcccgctc
     7321 cggagtccgc agccgggaaa tcatcaagag aggcaacctc acccagcttg aaatgaagca
     7381 cttcaagcag gcggtcaagg aagtgggagc ttcccagctt gtcattgacg ataccgcagc
     7441 catctccatc aacgaactcc gggccaaagc gcgccgtgtc atgagggatc agggaggtct
     7501 ggccgccatt ggtgtagact atctccagct catgcgctcc cactccaaac aggcggccaa
     7561 cagtcgtgaa cgggaagtgg cggaaatatc cgccggcctg aaagccctgg ccaaggaact
     7621 caaagtgccc gtcatcgttc tggcccagct caaccgcggc ccggaaagcc gcacgggagc
     7681 cagcctgggc gtcccccgca tctccgacct tcgtgaatcc ggctccatcg aacaggacgc
     7741 agacatgatc ggcctgctct accgctccgc ctactatgcg gaagatgagg aaaaacgcca
     7801 gcaaatggcc ggacgggcca atctgcatct ggccaaaaac cgcaacggcc ccacggggga
     7861 cgttccgctc cactttgaag cggaactcat gcgcttctcc acacgtgagg cagatgaaca
     7921 cgaccaggaa agcgaataaa acctctcgct gtctgtgcct gttctctgcg gattaacaat
     7981 ccggggtcaa gatttgattc taagagaaaa taacttgcgt agacgtttcc tttcatatca
     8041 gaggacgttt tcatgaaatc aagcccgact ggcatcatcg gtttccaatg aaaactcaac
     8101 agacctttgc ggtatttaaa tttgcaataa aattttgaga attccctttt ctggaaatag
     8161 ttacatgtag aaagaacgta tcatttcaat gatcgtagaa acttgtaact ttgcccgaat
     8221 taaacagaat tatcttcggt ttcagcctgt aacggattgt taaaccggta gcatcgcggc
     8281 aacgtcagca tttcttctgg ccttattgat caaattattt ctgtttttaa tatgaaaaaa
     8341 aacttattta ttgctgcaat cctctgcgga tcatgcatct ccgctaatgc cgccagcatg
     8401 gtcacggaat ggaccggcaa tgccggccct acggaaggca acacctatga actgggcaat
     8461 gcagacaact ggagcaacgg tgtacccgcc cgcggcaaca accagggacc ggatgtaatt
     8521 tttaataaca ctggaaccat caccctcagc ggctccatgg tggacacatc cgatggcggc
     8581 agcattactg ttacaggcaa cagcaatgtt acggtggggg gaacccgctg gacgggaaat
     8641 gtcaccattg gagcaggttc cgctctctcc ctctcacaag tggactttaa aagcagtgac
     8701 atcatcctgg acggcacctt caaccttggt gtctgcggga ttgattccgg cggcaacggc
     8761 gcccgccttg tcttcggcat tggaggcatc atgaacgtca accaaaaaat atggggggct
     8821 tcggacttct ccgtttccgg tacgctggcc accacatcca cggatctggc tgctggagaa
     8881 ttccagtttg ttacccgcac gctgattacc tccgccggct ttgacggcgg ttccatttcc
     8941 ctgggagact tcaccgctga agacggcgga acactgacca aggcatctgg aatcatggaa
     9001 gggaacgccg cagactacca gggccagtac tatctctaca cggaagacgg gaatgtcaaa
     9061 gtgcagtacg ttgtggcggg agccgtgccg gaacctgcta cggccaccct cagcctgctg
     9121 gggctggctt ccctgatgct gcgccgcagg cgcgcctgac cttttctccg gtccttttcc
     9181 tcaacagcct gtatgcccac gcgtacaggc tgttttgttt tccaggcgag tgacgaaaac
     9241 gtcacggaaa acatcttcat gccattgccg ggaatccgta gcatttttta ttatcttccg
     9301 ttcgacgtca tgcaagccca cgcaaaatca ctcaacgaac gtttccgcac cctcatctcc
     9361 ctgattttcg gtctggctat cggctgttcc ctgctcttcg gggaatcccg ctgggaaacg
     9421 tctcctctca ttgaagaaag cctcatgctc ctggcctgct tcatggccgg cataggcgcc
     9481 ttcgggcgca tctggtgctc cctctacatt gcaggttata aaaacaacgt cctggttact
     9541 gacgggccat actccatgtg ccgcaacccg ctctatttct tcagcttcgt tggcggcata
     9601 ggcgtatcct gcgctacgga gaccttcacc atccccctgc ttacggcgct ggcgttcgga
     9661 atctactacc cgtccgtcat cagaaaggaa caggaaaggc tcctcaccct tttcggagac
     9721 gcctaccgga actattgcag gaacgttccc tccttcattc cctccctctc acggctcaaa
     9781 ccgccgccgg ccgcctactc cgtcaatccc gccacgttca cgcacaacat tgtggatgcg
     9841 ctgtggttca tctggttcat cggcatcttt gaattcatca gcgggcttca tgaagccgga
     9901 atgcttcccg tatggttcct cattccgtaa caggctcttt tctcctggca tccagaagcc
     9961 ccgcaagcgc ccgttgggaa accattttac ggaaagatga gcggtcccgc ggataaagca
    10021 tcatctccgc ccgcgtctcc cccccccgga aagcccaggc aagccatacg gtccccacag
    10081 gcttttccgg cgtaccgcct ccgggccccg caataccgct gacggcaaca gcaacatcgg
    10141 ctccggagcg ttccagagct ccttccgcca tcgctttaac cacgggctca ctgacagctc
    10201 cgtaacgctt cagaacattc tccggaacgc ccagcatctc cgtctttgcc cggtcggcat
    10261 aagtaaccca tccgaattca aaaaccccgg aggcccccgg aacatccgta atcctcttgg
    10321 caatcaggcc gccggtgcag ctctccgccg tcgccgcctt cagaccctgc tgcgccaaat
    10381 ggcacacaac cgctttctcc agggatgccc cggcaggagc gagcacatac gccccggcca
    10441 gaatgcgcaa acgggcttct ccctgtttca gggccacctc actgcccacc aggcgcacgt
    10501 ccacttcccc gacgcgcgcg caatacgcta cctccaggcc ctccacctgg tgcagggaat
    10561 catccaccag ctcctggaga tcgctctccc ctatccctac caggcggaac acgcgcaaat
    10621 gaggaatatc ctcatccgcc aaatcttcca gaagcggcat cacggaatgt tccaccatcg
    10681 gcttcaactc cgccggcggc cccggcagca ggatgaacat gggaagggaa ccgctggcag
    10741 gcatcaccag tccgggcgcc gtcccattgg aattctccag caccgcggcg ccatccggaa
    10801 ccatggcctg cttgaaatta cactccgcaa tgggaatccc cctccgttca aagtaactct
    10861 tcagccactc agccacacac tcgtcacggt gcatgtccac gccgcacacg tcgcacaggg
    10921 cttcccgggt cacatcatcg ctcgtcggcc ccagtccccc gcttacaatc acgatatctg
    10981 cgcgccgggc gctctccctc atggcctcgt taatagcgta accgtcgggc acgacggtta
    11041 ccctccccac acggaacccg gcctcaaata atctgttgcc cagccatgcg gcatttgtat
    11101 tcacaatact ccccagcaaa atttccgtcc cggtgttgat caattcaacc ctgatctgtt
    11161 tcatgcagcc agtatgctcc ctcaagcacc ccgtgacatg caacaatcct gccatgtatc
    11221 ataaaaaact tcatttaaac tccccgtaag atacattagg ggcactgaca aagaccatga
    11281 acacaccttc ccggccccgg gccaccaaaa tcatctgcac tattggacca gcaacagaca
    11341 cttccgacat gctgggacag cttattgaag ccggagccaa cgtcttccgg ctcaatatga
    11401 gccattccaa acacgactgg gtccgggaag tcgtcttccg catccgccgg aaagcagcgg
    11461 agctggaagc gaacgtggcc atcctatttg acctccaggg tccctccatc cgtacgggag
    11521 accnnnnnnn nnnccgaacc ctaccatttg aaaaagggag acacggtaga aatccgcctg
    11581 ggcacggcca agccggaact tcctttttcc accacggtaa actatgacgg ccttctgcag
    11641 gatgttcagg cgggccatac catggtggtg gataacggag gcatcctcat gcgcattgcg
    11701 gaagtacgcc cggacaggct catctgcgac gtgctcacgg aaggcgtatt cggctcccgc
    11761 cgccacatca accttcccgg cgtagcgctg cggctcccgg ccctgacgga aaaggatctg
    11821 gcggacctgg ccgtggccgt agaatgcgaa acggactacg tagccatgtc cttcgtccgg
    11881 gatgccgccc acattgccca gctccgggaa cacatcgaca acttgggagg caaggcccag
    11941 atcgtcgcaa aaattgaaga ccagcaggct gtccgccaca ttgacgatat catcctggcc
    12001 gcagacgtca tcatggtcgc gcgcggggac ctgggcattg aagtcagcat tgaagaactg
    12061 cccatcatcc agcgccgcat cgtccgccat tgccacaggc tgggacgccg ctgcatcgta
    12121 gcaacgcata tgctggaatc catgataacc cagcccaccc ctacccgtgc ggaagtcacg
    12181 gatgtttcca acgccatctt tgaacaagtg gatgccgtca tgctttccgg agaaacctcc
    12241 gtgggccatt accccgtacg ctgtgtggaa gtgctggatt ccatcgccca ccgcatggag
    12301 tgctccggca atctcaactt cgcggcatcc gccatcctga acggagaccg ccagaaagcc
    12361 accaaggcag ccatctccct ggcggactcc gtggaaaacg cctgtctgat cgtctttacc
    12421 cgccgcggcc tggccgccac gcaggcagcc gtcctcaggc cggaacgggc tgccatcttc
    12481 gccttcagca atgaccctgt cgttgtcagg caactcgccc tggcgcgtga cgtaaccgcc
    12541 tttgaatctc ccttcctgaa agacccggcc cgcatgattt ccaccgccct ggacctgctg
    12601 aaggaaaaag ggctgatctc ctccggccag cctgtcgtca tccaggggga cagcctgcaa
    12661 ggggaactgc tggcggattc cattatcttc atgcgcgtgg aataatggcc tgcacctttc
    12721 ctatagagcg ggagacggcc gtgtggcccc cgtccgcccc cctcagggaa ctcctgctgg
    12781 atgcggatcc ggacgaacgc cggatagcgg atcacctggc ccacggcgaa ctttacgtcg
    12841 caaaaaactg cgggactgcc gccggagtca ttgttatccg tcagacgggg gaacatacat
    12901 gggaaatcat gaattgctcc gtagctcctg aatatagaag gcagcgttgc ggaacagccc
    12961 tggtgcggca cgccctggac atcatcagaa acaaaggggg ccggtatgcg gaagtgggaa
    13021 cgtccgatgc gtctccgggg ccaatggccc tgtatgaaag ctgcggattc caggtctatg
    13081 gcgttatcaa caaccacttc acggacaact atccggaacc cgtctgggac aacggcgtgc
    13141 aatgtattga catgatccgc atgcgtgcgg acctctccct tcggaaagaa ggagcgcatc
    13201 atactgctca gaaagcataa accacgctga ctcccgtcac ccagctattg ccctttacca
    13261 gcgttcttcc atacacgcgg ttcaccccgt gggaaccctt gccgagccag tgcaggccaa
    13321 caaaaggctc tataaaaaca ttcccgaaca gacggtaatt cgccgtcacc ctgaaataga
    13381 gggactgcca gccattgctg ctggaaggcc aataggaaga actcaacacg gcaccgaatt
    13441 taaaccccat ataaaggttt tccgtcatcc ggaaatccga tcccgtgcac atgtcaaacc
    13501 accagcccgt gattccgtaa aaggaatagg cggcacttcc ctgcgcataa aaaccgggga
    13561 ggaatcccac atcatggcgc ataatcagtg ccgcttcata cgttgtcccc ccattgccga
    13621 acaaacgttc ggaagcaaac cccgccaatc cgccgtcatt cgcacgaaag gataatgccg
    13681 catacgtagc ttttccgaac ttccgctgaa gaccaagatc caccaccgtc tcattctcta
    13741 tatcaaccgc cggatgcccc agaaagatct cctccttcag agccagaatc agggaatacc
    13801 ggtcattcaa atcgcttctc caatccagcc gcaaaggagc cgtgggatta cagcccgcgg
    13861 gaatcagccc cctgaactcg taattagtgg aatacccggc tttgatactg ccgctgaccg
    13921 gtcttaaagc aggagcggaa ggaacctccg gaatgcgtga ttccgccgaa gaaacggcca
    13981 tgaaagcgac ggaggccatg cccgctccaa ggagaaaaga aaaaaagaaa ttcataacat
    14041 cggaatgccg tacggaaagt attctggata aaaaaactcc tcttggcaac aagcaaaaac
    14101 gccgctgttt cctgctctgc ttcaggaaac atcattcagg cctgcccgct gccaacttca
    14161 gaaaaaataa acgagaccag ccccggccac tactccgaaa ttcttataag gcttgtcgcc
    14221 cctgtccgaa cctcggttgg ccttcaaggc gccctctccg gcccagttga agctgacgaa
    14281 gggaacgaga gacaggttct tggcacccag cttcaccgga agttcagcct tcagccacca
    14341 tgcctgggaa ccgttggaca taaaaaggga tttattgtca aaataagagg aagtggcaga
    14401 catcccggcg ctcaatacaa aatcggcgtc ggagcagata gcagcctgat accccacatg
    14461 cggccggaac caccagccgg tcataccctg gaaggcatag ctcatggtta cgccggcaaa
    14521 ccagtttccg ggcaaaccgt aatccagcgt cagataaaat tcctgaatga cggaatgggt
    14581 attccttcta tacacatctt catcactcat ccaggtggcg ttataatcat acctggcaaa
    14641 attcccaagc aggccgccgt gaatcaggtt ccagccaagc gtcgtggaca ggttcttcag
    14701 taaaccgtcc cggttcttcc atccaaaaag aaaatcagtc tcgttatgaa aagagaaatc
    14761 tctttcccct gccatgtggt gtcctgaaat caggaacgta caagatgccg ccccaacaac
    14821 ggaattacaa tcatccagct tataattcaa atccacactg gcaggaataa ttccatctcc
    14881 ttccgtcagt gaatgagaag ccacaatgcc gcggcaggtg taattggaag catacccggc
    14941 attaacttcc caggacaaag gacagtcctg ttccacggga acagaaaccg gagaaacagt
    15001 tttaacggaa attcctgcaa aagcggaagc ggcaagaaaa acggcacccc ccgcaacacc
    15061 aatgatattt ctgaattgca tcatcacgct tcaaatgaaa atttggatat cggcgcattg
    15121 caaaaaacgg agttcataaa aaacaatgcg ccgccatcaa ttaaaaaaag cgcggcggat
    15181 ttctccgccg cacctgaaaa tataattcaa tcgcaggccg gatggcttag aaagaataga
    15241 caaggctggc tccagccact acgccgaagt tcttgtaagg cttgtctccc ttatccaggc
    15301 ccttattgac cttcaacgcg ccgcagccgg cccagttgaa gctgacgaag ggaacaaggg
    15361 acaggttctt cactcccagc ttcaccggaa gttcagcttt cacccaccag gcctgagcgc
    15421 cattggcctg ctcgaacgca gcgccaaaat agctggacgt ggcagacatc ccagccgtca
    15481 cgacaatgtc cgtgacgggg cagagggcgg ccttgtagcc aacataaggt tggaaccacc
    15541 agccggtcat gccctggaag gcatagctcg tggttacgcc ggcaaaccag ttctgagtca
    15601 gatcgtaatt cagattcagg taaaattcct gcgtgacgga atgagcatgg tcatacttgg
    15661 caaagctgcc cagcaaaccg ccgtgaatca ggttccagcc aagcgtcgtg gacaggttct
    15721 tcagcaaacc gtccttattc tgccagccaa ggttgaaatt ggtctcattg tggaaggaag
    15781 tatccctgtc ccccatgaga tggtggcctg aagtcaaggt cgtataagaa gcggaagcca
    15841 cgatggagtt ggcatcgcac agcttgtaat tcagattgac accggcgggg atgacgctat
    15901 ccccttccgc caacgcgtgg gaagccacaa tgccgcggca ggtgtaattg gaagcgtagc
    15961 cggcgtaaat atcgccggac agcgggctgg aagtcgccac aggagcggga accggagtcg
    16021 gagcaacaat cttgacggga gtacccgcaa gagcagctcc ggcaaaggat gccacaactg
    16081 ccgcaacact gatgatgttc ttggtttgca tgactattac ttggttaggg tattaattgt
    16141 tgatttgcta acatggaaag aaaaatgctt tttcccccct tgttaagaaa agtgaaacat
    16201 tttcgtcaca aaagtaaaga attttctctt gataaatcag caagccattg atttcacatt
    16261 atttacatcc accagcccca tgacaaaaaa tccaattctc catatcttgc caaaccttcc
    16321 cttccaaggc ctggccggat acagacactc cgcaccatcc agaagcctgt cttctttcag
    16381 ggcaggagaa ggattcacca gaaccctccg cctgcaggaa agcagcatgg gcagatccgc
    16441 ggcactgtcg ctgtaggcaa cggcgttttc cagaacacca tgttccggca gtacattccg
    16501 ctcccgcaga cgctccacct tcaccgcccc cttattatta ccgttcggca gttccggcat
    16561 tgccggcatg cgttcctcca gaagcacatc cgttcccaga acttcgtcaa atcccagcag
    16621 ttcgcccaga ggcttcacgt aaaatgatgg agaagcggaa accatgagac acaaataacc
    16681 cttcttccga taccctgcca gccgctcctt cagctccgga taaatccatt cctgcgccat
    16741 ctcggcaaac ttccgtccat attcccgcac cgtttcagcg ggaagccccc acaaatacat
    16801 gagataagcc ctcttcatcc gattctcatc ccatattctc aaaatataga gcggaataca
    16861 ggccaggaaa agaaaaatca gcaagcgtct ccaaggatgg cgcctcacca caaaacagga
    16921 aaaaacatat tgcgtatccc agggaaagag cgtgccgtcc atatcaaaca gcgctgcgcc
    16981 tctttttttc caatcgtccg tcatgctttc atcctatacc cgcttccctt aactctcaac
    17041 acccaactct tccatgcatg cccctcctcc tgaatgccca aaaccccggc cgccgaaagg
    17101 caaccggggt tttgaagcaa atccagaagg aaataagatt aacgggagta aaattccacg
    17161 atgagctgct cgttgacgat gggagcaatc tcttccttgg aaggcacgcg ggcaatcttg
    17221 cccgtgagct tgtcacggtc gcattccagc cagtcaggaa ccacagtagc ctgagtcagg
    17281 tcaaggaaac gggtaaccag ctgctgggaa gaagccttgc cgcctacggc aatgacgtcg
    17341 cccggacggc aggagtagga cgcgatattc gtcttcttgc cgttcacggt gatgtggccg
    17401 tgggaaacaa gctgacgggc gccggcacgg gtattgctga agccgaggcg gnnnnnnnnn
    17461 naggcggtac acaacattgt ccagacgaag ctcaaggagc tgaagcagaa tatcgccggt
    17521 aatgccgcgg cggcgggcgg cttctgcata atacttgcgg aactgacctt caagcacacc
    17581 gtactggaaa cgcagtttct gtttttcagc aagcatcacg ccatagtcgg atttcttctt
    17641 gcggccggcg cgcatgccat gctggccggg agggaagggg cgcttttcaa gagccttggt
    17701 ggaaccgaaa agggcaacgc caaaacggcg ggacacttta tcgcggggac cggtataacg
    17761 agccatgata tattagatct atataggtaa aagattctca attagacgcg gcgggccttc
    17821 ggaggacggc agccgttgtg ggggatgggg gtcacgtcct tgatggaggt gatttcaata
    17881 ccgatcgtct gcacggcacg tacggcggat tcacgaccgg aaccgggccc ctttacgcgc
    17941 acttccactt cacgcaagcc gtgacccatg gcctggcggc aggcgtcctg gcacaccacc
    18001 tgcccggcat aggccgtgct cnnnnnnnnn ntgctcttgc gggaaccctt aaagcccatc
    18061 ttgccggcgg aggaccagcc gatgacattg ccgcgctggt cagtcacagt tacgacggtg
    18121 ttattgaacg tggaggaaac gtgaaccacg cccgtggaaa cgtttttgct gcccttggcc
    18181 ttcaggattt tgggcttgtc atcatcgccg cccagaccaa gttccgcaaa gatatccttg
    18241 cggggttcgg gcttcttgat ttcttcagga gcggaaacag cggcggccgg agtttcagcg
    18301 acgggagcgg aagctgccac ttccacaggc tgttcggcgg tttcgttggt gatttcttcg
    18361 ctagccatag taaagctata ttaaaaattc aggttggcgg attacttctt cttggcctgc
    18421 gcgccaacgg tcttcttctt gcctttacgg gtgcgggcat tcgtgcgggt acgctgaccg
    18481 cgtaccggaa ggccgcggcg gtggcgctga ccacgatagc agttgatgga ggtcagacgc
    18541 ttgagaatgg attgcttttc acgacgaagg tcaccttcaa tcaaaatgcc gtcggtggtg
    18601 attacttgaa caatcttcgt gagctgttcg tcggtaagct gtcccgtgcg gatgtcggga
    18661 ttgatgcctg cttgttccag gattctctta gaagtcgagc gaccaattcc gtaaatatac
    18721 ggaagggaag cctcgatgcg cttctcgttg ggtatttctg taccgaaaag gcgtgccata
    18781 atggattgga tttgctagct gttatttttc tctgcctcgc gcagagctat tgcttggggc
    18841 aagatggata ccataaattg aggttttgac aagaccaaaa catcattttt tctattttta
    18901 atctcttgca ccgcttgaag atgcatgccg cccgtggcgg cggaggatgc gctccacagc
    18961 ccaggaagcg tgttcctcca ccagaggaga ctccccgtaa agcgccttca gaaaatcaat
    19021 gtcatccgga gtccccgcat tccccagcac aacgcagccg ttacgcagca gcccttccct
    19081 cttgagcctt ttcaacgggg aattccggaa cagggccgca aaaccgccgg catccaggga
    19141 aagaagaccg cgcaggggaa tggaagccag cagacgggac atgcggaacc gttcgtcaac
    19201 gtccggcaaa ggcttaccgt tccagggaca gaccgtcacg caggtatcgc acccgtacag
    19261 gcgcgtgcct atcaggggac ggatttcttc cggaatagcc ccccggtgtt caatcgtcca
    19321 gtaggaaagg cagcgtccgg catcaacctg accgttttcc ataattgcgc ccgtcggaca
    19381 cagggccgca caacggcggc aactgccgca tccgtttgaa accggagcgt ccggttccag
    19441 ttccagcgta gtcagcaggg tagcgataaa aaagcgggag ccccctttcc tgcgcacgat
    19501 caaaccgctt ttcccgcgcc agcccaggcc gcaggcctcc gcatggtccc gttccataac
    19561 agggccggag tccacatacc ccctctgctt cccgccgtaa atggaaagca gctcctgcaa
    19621 atccgccagt ttttcctcca gaattccgtg gtaatccttt ccatgggcgt aaaggcaaat
    19681 gctaccctcc ccctccgggc gcctaccggg gctgtcataa tcataggaca ggcaaatgac
    19741 ggaacggcat ccgggaagca cttccgcagg atccgtgcgc cgctccgggg aaagcgccat
    19801 ccactccatc ccggcgtgcc atccgcgctc cagccattga aacaattttt ccgcatgcgg
    19861 gcttttttcc gcacgggcca cccggcagcc ggaaaaacca agctgccggg acacggcgca
    19921 caaggaatct ttaataacag aggaatccgg agtcagcacg ccggaatcat gaacaagagc
    19981 atgcctccca ccaagaattt tcttctgtcc ggtggaaatt ggattgcctg caccccgccg
    20041 ccgcttcata atgccgcata tatgaacatc cggattctga gcgatgggaa acagggccac
    20101 ctcaaccagt ccctggggct ggcgcaggct ctgatctcca aagcgggggg aaccgtggaa
    20161 acggtagacc tgcaggggct ttcccttttg gggaaaatcc ggaaggtcgt cacaggcagc
    20221 gacattccac ggccggatct gttcatttcc gccggacatg caacgcatat cccgctcatc
    20281 tgcgcgcggc accatttcaa aacccgggcc gtcctctgca tgaagcctac gcttccctgc
    20341 tcctttttcg acctctgcct tattccccgc catgacctgg attccggccg cgattacacg
    20401 gatacggata tcttccccac ccagggagct ctccacccca tgagaccgga tccctccgta
    20461 ccaaaggata ccacactgat tctcatagga gggcccagca aagattttga ctgggatgac
    20521 gaaagcatgc tcaaccagct cgcgtccatc agcatccaca cccccggaca ccttgttcta
    20581 accacctcgc gccggacgcc ggacggcttt gcggaaaaaa tccggacggc cgttccggaa
    20641 ctaaccgtcg ttcccgtgga ggaaacccgg ccggggtggg tagcccggca tctggcgcac
    20701 gcctccgccg cctgggtcag ccaggacagc gtctccatgg tatatgaggc tcttggctcc
    20761 ggcgcgcctg taggcatcct ttccgtaccg cgccgacacg gcagccgcaa atcccgcatc
    20821 ctgtccggcc tggagacact ggaaaaggaa ggcatggtta ctggatacag tgactggaaa
    20881 aaacagaact tccgcctgac ggctcccggt tccccgcttc tggaagcgga ccgtgcagca
    20941 gactacatcc tctccagatt tttccctcag ctccgcgcct tatgaaaata gtacatctcg
    21001 tcccctccat ggaatccgga ggcgtggaac aagtcgttat ggaactgggc agcggccttt
    21061 cttcccgggg cgtggaaaat atcgtcgttt ccggaggcgg acgcctggtg ccccgtctgg
    21121 aaaaggaagg ctcccgccac atcctgatgc cgataggcaa aaaaagcatc tccactctct
    21181 tccgcatcgg ggccctccgc gccctgcttc aggccgtcag gccggatatc ctgcatctcc
    21241 attcccgcgt tcctgcgtgg gcaggctacc tggcatggaa aaagctcccg ccggaagacc
    21301 gccccggcct cgtcaccagc gttcacggct tctactccgt caaccggtac tccgccatca
    21361 tgagccgagg agagcgggtg atcgccgtct ccaactgcat cagggactac atccttgacc
    21421 attatccgtc cacccctccg gaccatatca gaatcatacc caatgctatt tccccggacc
    21481 aatatcaccc ggcctactcc ccctcccggg aatggctcac gggctggttc atgtcctatc
    21541 ctgaactgaa ggggaaattc accctgtgcc tgccgggccg catcacgcgc ttgaaagggc
    21601 atctggatct gattccggtc gtcaggcagc ttctggaaca gggaatcccg gcccacgccg
    21661 tcattgtagg agaagcaaag aagggaaaag aagaatataa aaacgaggtc ctgcgggcaa
    21721 tagaacgttc cggcgtctcc cagtccttca cctggacagg ccatcgccag gatctgaggg
    21781 aaatcctttc cacatgttcc gtcaccctct ccctgaccaa aagcccggaa gccttcggca
    21841 aatcaaccct ggaggcgctc gccctgggca aacccgtagc cggatacgcc cacggcggag
    21901 tcaaggaaca gctggacgcc ttccttcctg aagggaacgt cgccgtagga gataccgccg
    21961 ccatggcgaa cctgctggcc cgctggcata cccagccccc ccccctgccc cggcaaattc
    22021 cttccccgta caaaattcag ggtatgattc aagcccaaca ggacgtttac caggaactga
    22081 cactttattc atgacacccc gggaagcagc catagccctg aacctgatac cgggcctggg
    22141 ccctgtcaga atcatgcgtc tcctgcaggt attcgcctct ccggaactga ttctggaatc
    22201 cccttcctcc ctgttgatgg aaattcccgg cgtgggggcg cagctggctt gccgcatttc
    22261 ctcttggcgg agcaccgtca acccgtacag ggaactggaa ctggcggata acgcgggggc
    22321 cgcggtaacc acggtttttg acgactccta cccatcctcc ctgcgtgctc tcccagaccc
    22381 gcccattgtc ctctactcct ggggaaactg gaccggaacg gatgccgaac gctccattgc
    22441 cgtcgtcggc tcacggatgg ccacccatta cggcaggctt tgcgccagaa acatctccca
    22501 tgacctggcg gaagccggaa taactgtcat ttccggactg gcgcgcggcg tggacacgga
    22561 agcccatacc ggggccatgg acgcggaggg gcgcaccatc gccgttatcg gggcaggtct
    22621 caacaaactg tatcctcggg aaaacagaaa cttggcgcag cgcattgcgg acgggcatgg
    22681 agcggtagtc tccgagttcc cgatggacct gcccccctcc cgcaccactt ttcccatgcg
    22741 caaccgcatc gtgagcggct ggagccgcgc tacgctggtg gtggaggcgt ccggacgcag
    22801 cggggccctg atcacggccc gaacggcggc cgaacagggt cgagacgttt tttgcattcc
    22861 cggaccggtt gaccggcatt cttccgacgg atgccatgcc ctcatccggg acggagccat
    22921 cctagctacc ggagcctccg atattctgca ggacatgaac tgggccgttc cggaacaggg
    22981 actgcctctc ttctcgccat gctcccctgc cggagcctca accccaccgc ttcccacttt
    23041 ggaagaaaag gagattctcc acgccatcag actgggtttc aatactattg acaccctctg
    23101 cacct
//
LOCUS       DAWEMX010000027        22061 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_985_length_22061_cov_10.030537, whole genome shotgun sequence.
ACCESSION   DAWEMX010000027 DAWEMX010000000
VERSION     DAWEMX010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_22061_cov_10.030537"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4926..4935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6823..6832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5153 a         6467 c         5803 g         4618 t
ORIGIN      
        1 gtaaatatcg cgcagcacat ctgtccggat gatatcttcc gtcggccctt gatggcacag
       61 gattccgtcc ctgaatgcca ccacgtaatc cgcatagaaa gagacaaagt taatatcatg
      121 aattacgatg cagatcgtct tgccctgctc ccgcaccagg gaacgcagca gtttcattat
      181 gcgcgccgca cgatgcatat caaggttgtt caaaggttca tcgagaaaga tgtacttcgt
      241 gtcctgtgca atggccatgg caatataggc catctgcctc tgaccgcccg aaagctcatc
      301 aaggtagcaa tggcgcaaat cggaaatctc ggtaagttcc aaagcctttt cgatttgcag
      361 aaaatcctga ttttccaggc ggccgcggga atgagggaaa cgtccgaagc gaaccagttc
      421 ctcgaccgtg agccgcgccg gagtatggag ggtttgcccc aggatggtga gggttttggc
      481 aagctcccgg gaatcccatt tcctgagttc cctgccatca atggatacag agccttcggt
      541 gggttccaga aggcggctga taatgttgag catcgtgctc tttccggcgc cattgctgcc
      601 gatgaaggcc accatccgat cagagggaag cctcgtgctg acgtgcctga gcacgaaggt
      661 ttcacggtat tttttggaaa tgtctcgtat ctcaatcata tacgtttaat tttcatgatc
      721 aggtagataa agtaaacccc gccaacgaaa ttgatgatta cgctcagggg cacggagaag
      781 ttgagccacc gttcggtaaa cagcatgccg aaagtcaatg cggctacgcc cacgagagcc
      841 gtgccgggca tgaggacgat gtggcggtag gtcgggaata tgaagcgtgc aatgcttacg
      901 atgagaatgc ccaggaaagt caccggccct acgagcaccg tcgaggccga ggccagaata
      961 gagacgatca tcagggagcg cagcaccacc catttgtagt tcacgcccag cgtaatggcc
     1021 gtggagcggc ccagcgtaag cacgtcgagc ttcctgaggt cgcaaaccag ccagatgacc
     1081 gccgcggcaa tcacaagccc tgccgtaagc agcagttctt cattgatgcg gttgaaactg
     1141 acgaaaaggc gcccctcaag tatggcaaat tcgctcggat ctatcagcac ctgcatgaag
     1201 ttcgacaagc ctccgaaggt tatgccgaag atcatgccta ccaacaccac aaaataaata
     1261 ttgccgccat tctgtccacg gaacatgtag aaaaaaatac ccgtagatac gcagaccatc
     1321 agcagcaggg ctcccatgaa acagagcggg ctctgcatgg tcgtcaattg tgtgctgccc
     1381 acgaagtaga gcacgagcgt ctgcagtaat acgtagaggt tatcgagccc catcacgctg
     1441 ggcgtgagga tatagttggt tgtgatcgtc tggaagacga ctgaagacag ccccacggcc
     1501 gttccaacca gcaccaacgc aataacgatg ggaatgcggc gggccatgtt aaagtcccac
     1561 gtttcgggtg tcatcccctg aaagacatat acgagcgcca gtcccacgca gagcagtccc
     1621 agaatcagga aggccgggaa cgcgcgacgc cggccggcag cattgcggaa ggcatcatct
     1681 ttttgatgtt gaatggaatc aggcatacgt ttttctatgg aagagcatgg tcagaaatac
     1741 aacaccgccg accacgctca ggataacgcc gatagggatt tcgaaggggg cgatgagcat
     1801 gcgccctgcc acgtcgcaaa caagaacaag catggccccc agccaagccg tatcaaacaa
     1861 gattttcttc acggcatccc cccggaaaat gctcacgata ttgggcacga tcaacccgat
     1921 gaaggggagg tagcctacct ccacaatcgt gattgagctg atgatggata ctatcaccag
     1981 tcccatgcgc atgatgcttg cgtggttcag ccccaacgcc accgcggcat cacgccccat
     2041 gccggcaatc gtaaagaaat cggcataaac ataggccagc agcatcaacg ggatgctggc
     2101 ataaagcaat tcataattgc cctcaatgac aagggagaaa ctcccctgca gccatgatgc
     2161 catattctgt acaatatcaa actggtaggc ataaaaaacg gctccagcat tcaccacgcc
     2221 gcccagcatg attcctatca ggggcaccag aatgacattc tggtgccgca cgcgcgcaat
     2281 caccgccaga aagcatgagg tgcctatcag agaaatcacg aaggcggccc ccacgcgcca
     2341 aatgggagag gccccgggca gataaaggat ggcgaccatt accccaaagc ggcaccactc
     2401 catcgtgcct gccgtggtcg gcgacacgaa gcggttattg gtgatgcgct gcatgatcaa
     2461 gccgctgatg gccatgctgc tgcctaccac gagaatgctg cagaggcgcg gaatgcggct
     2521 ttgcaacatg atctcccacc agaactgcag ttgctcgcca tccatccgcc acaggtcgga
     2581 caaggacact tgagctgccc caataaagag cgaataaaag gcaaggccaa gcagaaagct
     2641 ccactgaatg attctgaatg ccattttctc aggctttcaa cacggctgca tcaagttcca
     2701 tttcaaacaa cagattgtcc cggcacacat cggcctgtat gccaatgcag ggcatgatgg
     2761 cgaggccgcg gcgcagagct attttttcgt agtgaggggc ataatccttg tctttgaaat
     2821 aggctacgga gcggacgaca tccttccagc ccatgccgcg tgatttcagg atggcttcaa
     2881 tcacgtccag cgagagctct gtttgtttgt cgatatcgcc gagatgtacg gattcgccgc
     2941 caggggcaat gctggccgtg ccggagacga tgacttgccg gaagagaggg tggtccactt
     3001 ctaccgcacg gctgaatgag ctgcggtaat cctgagctga acattgcaga ggcgaaggga
     3061 tatcttttac cgtcacacgg tcgcccttag gccgaatggc aaatacgcgg gccgacaagg
     3121 ccgcatgttc ccgattgccg gaaccaatcc ccgtactggc cggaaccaga cgatcaaaca
     3181 cgccgcggct ttcaaaaaat gtgtcgcggg cgcggttgaa ctcttcatac cagtccagca
     3241 ggcggttgtt gtaaaaccat gtgcggacca cgtgggaaaa atccatgccg gcccggttca
     3301 gcgcttcttc catctgccgg aatacatcca atgtctgctc agtccgtccg gctgtccggt
     3361 cgcggggcga caaaccgccc aaaacggcat actcggcata ttcatcctca taaaaaaccc
     3421 cggcggtgcg gccattaaac tcaatcgccc gggcatttat cccctccacc gcgcaaattt
     3481 gcatgcctcg atagaaggat gcgcccgttt tcgtacccaa aaaggcagtc atccccgccc
     3541 cgggcaccga ggagagatcc agatcagcgc tgccgtacac ttccgggcca atccaccgaa
     3601 aaccgctttt ttcagcttct tggagtaatt cggcatgctc ggccaactgc ccaggttcgc
     3661 ttccggcaag cgtaacgaat tgcagagatg cgccttcgcc gcccagcggg agcgacttga
     3721 cggaaacacc tttatttttt tccaagttgc aggtcatgag tatattattt ttttaaagtg
     3781 ctttcaggaa ttgaatcagt tggccgcgct cttcggcgga gagatggcgg aacatttcac
     3841 gggagaattc gctttcaccg aaatgccaca ggatagcctc ttcaaagtcg cgggcgcgca
     3901 ggtcatggaa catatctgta tgattggcgg ttttgtgcat cagccccctt ccccagaggg
     3961 gcggcgtacg ccagaaacga gaacgtccgc ggttgatttc ccccatatcg tgacgcagca
     4021 tatccgtata ggggcggatg gtctggtttg cataaccggg aagcggctta tattcccccg
     4081 ttgtccattc aggcttgtgg caaccgacac accccagttt attgaagagt tcctgtccgc
     4141 ggcgcacgtc aggcttgtcc aggttgcggg cggcaggcac ggccagcccg cgatgccaga
     4201 ccatgaaatc ttcatactgc tgcattgaga gttcctcttc ctgcgggcct tcaagaccgc
     4261 ttacatcaat gccaagttcc ttctgttttt cgagccattc gggcgtcatg tagatgttcg
     4321 gacggttctt gcgcgttaca ttcgtcacgt tccaaagcgc attggcgcca ggaccgtttt
     4381 ccagtgtggc gcgcgagcag tcccacgtga actttccgag gcgccgggta ccgtcgggct
     4441 cgtcaatcca tttgccagga atccccttca ccgggcctgt catgctctgc tggcgcctgt
     4501 attcggcaat aatatcctcg tcacggatgg catcaagcag ccccgttccg taaatgccga
     4561 ttgttgattc gattgacacg cggtaatcgg ccggaagcgg aagcaacggc tcaatcacat
     4621 ctgccgtggg ataagtcaac gagccttccg tcggcttgcc cgcattgtag ggcgttccgt
     4681 ccgggtattt gtttccgtac tggtcaacaa aatcatgcca ttctattttc acccccttgg
     4741 cataaggcac aaaaggagga accgctttcg tctgcagcat gcccgtgtac cccttaaccg
     4801 gggtgccatc gggattatgt acaaaggcca gataaccgtt gccgtactgc ctgtcaaaat
     4861 tgccggtgcg ctgtccgcgg ccataaccgg ggtggcaggc cagacaggat gacttaatgt
     4921 aagtgnnnnn nnnnngagac cgccgaagcg attgcgcttc acgggtacga agttcccttc
     4981 aaaaataatt tcgcccgcct tgaaacgatt cgtcaggccg gcttcagtaa ttgccggcga
     5041 ttcctgctca aacgctttac tggtggcatt aaacactgtt cccttttcac cgcccagata
     5101 gtaagccggc ccgggctcgc cttctttcgt cctgagcacg gggggcgtga aatcacggcc
     5161 gcggtttaca tggcagctca cgcatgaagc tgtcatgtat tcggctttga tattgtcaaa
     5221 ttttgatgca ctcccctggg ccggattaat cagtgcgcgt ccggaaatgc ggggatgtcc
     5281 caaaacgccg gcattccttg ccgtgtccgg gataaaagat tgttcaggag ccgcatcccc
     5341 agaacctccg gaattcacta ttgcccaggc ggccgctccc gctccgagca ccaggacagc
     5401 cgcaggtttt gcaaattgtc gatactccat gtgcattgat gattggcagg ctttccagaa
     5461 atacggcccg tggtacatgt ctccgtcagc cggccgacaa tatggatatt gcagcccagg
     5521 gtctttccga ccctgggcaa tgcgtgttaa attacttcac aagaagttcc tgaaccttgt
     5581 cgaggttttc ggaaagttcc ttgcaactgt cgatgattgc cttgagctgc tcctttttgt
     5641 cgaggttttt caccaggggg ccctgtatgg cgtcgaggct cttgaatacc ttggcgatag
     5701 aacctttcac agcctggtca accttcgcgg acttggcggc tacgagaggg gaaatcacat
     5761 tgtaggattt ttctacgctc tgtacgttgt ggcgggagtc caccagagaa gtgtagctgt
     5821 aaagggattc acagtcggcg gcgtcagccg tttcgtaggg cttgcccacc tttgaatcgg
     5881 ctatttcgtc aataatgtcc ttgctgccgc cgatgatttc atcaagcact tcagagtttg
     5941 actcgtagcg gctacctgcc tggccagctt ttttgaattc ccccgcatag gacttgccgg
     6001 gttctatttc agcttcttca agaatcttgg ccttctcttc tctgatcttg tcagaacccg
     6061 cccaccaggc ttcaagcgtg atggcatcct ctgcaatcac ctcggccaca gcggcaaggt
     6121 aggaaagctg tccgggcgtc agatcggcgg ccttgcgggg ttgcccatct cggaacaaga
     6181 ggtattcggc agcatggaaa ccgcgcgttt ccgcgccgag ggaattgcgg acgtaggcgg
     6241 cgtcaatcgt tacgctcttg ctgtccgcgt ttttggcgac ggcgtcaagg ttcgtggtat
     6301 ccaggggcca ggagtccagc ttcgggtcaa ggttggcgaa agcagccgga ccaaagagga
     6361 aggattctga aagttcccac ggcacgcgcg tttcaagaag aagcttccct gcttcagcaa
     6421 ccttggcatc ggtaggagca gccttcagct ctttagcggc cttggcgaat ttgagagcgt
     6481 tatcactcat cgtcttgtag gtgggaatga caagctcatc cgcgtaggcg gcaatcgccg
     6541 cgctcgtttt gtccaccttg ggggaagcgg ccttggccgc ctgagcctgg ctaccgaaag
     6601 ccaggattgt acttccggcg caaaagccgg ccagcaacat gcgtttgtat gcgttcatgg
     6661 ttttttatta ataatgaaaa catccgtatc ctcctcaggg aggcatcctc caatccggca
     6721 ggatttcatg gcatatccgc cattttttcc cgccggagcg gaaaataata atccggccaa
     6781 acgatgtcaa ttaaaaatta gtttaaacta actttgcaac aannnnnnnn nnaaacacct
     6841 cttgtggccg catgccggaa catccggcat gttattgatt cggaaatatt ttctgtttcc
     6901 gaatgcgccc aggagccgcc tttcaccaaa ttccgagttt ctccgcgaca aggctcctta
     6961 cctcgccaaa agatttcact acgggcggat tttccgtatg gcctccaaga aacttctcca
     7021 tccagatcat atggtaccag cggccgaatt tgtatccgca ccgatgaaat ttccctgcaa
     7081 attgataccc caaacgttta tggaactgga cgctgtcccg tgtaagatag gggtcctcag
     7141 cttcacaggg ataagcaatg cacgcgttta aattcaaaat attctgcatg gagagaatct
     7201 tttccagagc tgcatacagt tcccttcccg ttcccatttt tttcatgtct tcccgcacgt
     7261 ataccgtcgt ttccacagcc cagcgatacg ctgcgcggtc cttgaacact cctgcatagg
     7321 cataaccgga aatatcccct gccctttccg caaccaaata ggggtatttt cccagtatgt
     7381 gatctattct tttttcaaac tccgtcactg caggcacttc atattcaaac gtgacagctg
     7441 tcttttccac atacggagcg taaatttcca gcagcttggc cgcatcccgc ctcgttgcaa
     7501 ttctgattct gctgcttttc atttcggttg actgtgctgg gcccctgagc cggaagcgct
     7561 gccgatcatt ctaatatttc acaagcgatt gtcaatttga ggccgcccaa gcacccgcca
     7621 tttccgagaa atatcatccg ccgcatgttt tccgccgttc ccgttcatga acgggaagaa
     7681 acggggtcaa tattcatttc aaaatcagag taaaaaccgc attttttacc ttgtccggta
     7741 ccccgtgtcc atttatggaa agccctcccg aaaaagtgaa aaaaggccgg agtccataga
     7801 gaatccggtc ttttggaaag gataggatgc cacttcgcgg cgaaatccgt ctcaggagta
     7861 aaaaccgatg tccggttccc ggtcccgccg cctgcgttta aaatacggat tgcggtagcg
     7921 gtggatgcat ttgccggaac tcccaggggt ataatgaaga ggggccgcct ccttgacggc
     7981 ttcacggctc aatatctcca caaaatcctt caaggacatt tccagatctg catcgtggca
     8041 tgccagcgcg gcggacaggc gcactttgcc gttgtcatag tccacataca tggcgaacag
     8101 cgtggtctgg tacaggtgcc acggttcccc gcccattttt ccggactggt gttggctgat
     8161 ggcctccaac agccacgtca gcgcatgttc cagttccaga ttgtccagaa catcccgtat
     8221 aagagatgtt tcgcggccga aaacggccac gctttcctgt tgtcttgccc tgttgatttt
     8281 catgtcttgg ttctcctttc cggtttccct tgttaagcag aacggcggat aattctgccg
     8341 ctccaattgt tttggacact ttttttcaac aggttgttca atctgaattc tcaactgtgt
     8401 cagccatgac cactttccca atgtacggga gttcccggta atgcccatcc aggtccattc
     8461 caaaccccac caggaaatcg tttccctgcg taaaagccac gtaatcagcc tcaaaaggca
     8521 ctttccggca acagtgcttg tccacggcta cagcagtgag gacttccttc acccctcttc
     8581 ttttcaattc ccggcaaact tcatccagcg tagcacccgt atccagaacg tcatccacca
     8641 ccagaacagg cacatcgggc tggaaatccc cgcaatcccc ctcccacgcc acggaaccgg
     8701 aatttctcat tccggcataa ctggaaattt tgacggcttt caccgcaggc cgggatttca
     8761 aatgcttctc caacatcgca gcgaaaggaa gagcgccgtt cagcagcagc aataaaatat
     8821 agggttttcc tgccatggcc ctgtcaatgg atgcggccag ttccctgacg cgcgcagatg
     8881 gcatcctctg caatcaaagg agtgatggtg taagtcatgg aaacggagca tgataatgtt
     8941 tcctgtaaga aaatcaatcc ttaaagaagg atgaacggag acaaaacatt cccggccggg
     9001 ctccagcaaa ttttacaaga agcaggactg gtcttcatag tcaggaggca acaaaaaagc
     9061 gctctcaaga ttgtttctcc gccccgatcg gcagagaatg cggaggcgca tttaacggtg
     9121 gcttcccgcc ggaccgcatg gtaaagtaag gcagaaatga ctgattcccc ttccaagcgc
     9181 ctttttattc tggatggaat ggccctggct tacagagccc acttcgcctt tttctccaat
     9241 cccatccgca attccaaggg agtcaatact tccgccgtgt acggcttcgc caatacgctg
     9301 ctgggcattc tggagcacga acgccccacg cacatcgcgg cctgtttcga cacttccgct
     9361 cccacggcgc gccataagct ttaccctgcc tataaagcca accgggaatc catgccggaa
     9421 gagttgagcg accaaatgcc cctgattttc agattgctgg aagccatgaa tattcccatt
     9481 ctgcgctatg aaggctatga ggcggacgat acgataggca cgctggcacg catcgcggac
     9541 ggtacggagg gattccagac ctacatggtt tcccaggaca aggacctggg ccagcttatt
     9601 tcctccacct gctttctgtg gaaacccggc aaaaggggca atgaccacga agtgattgac
     9661 ctggcaaagc tcaaggagca atggggcatt gaacgtgcgg accaggtagt tgatattctg
     9721 gccctgatgg gcgacagctc cgacaatatt ccggggcttc ccggcgtggg ggaaaagacg
     9781 gccaagctgc tgatcagaga gttcggctcc gtggaaaacc tgctgtctaa tacggataaa
     9841 ctgaaaggga agcgcaaaca gattgtggag gaaaacgggg acatggcaac cctttccaag
     9901 caactggcca ccattgaccg gaacgttccc ctgacggtga ccctgcctga attggttaaa
     9961 agagaaccca gtccggaaga actgcaggcc cttctccagg agttggaatt ccggtccatg
    10021 caggccaagc tgttcgggaa aaaagcgccg gagcccagaa aagcccccct cccggcagac
    10081 gatttgtttg ctcccgcccc ccggacggaa cagccttcgt cggcgaaacc ctccgcccct
    10141 gtgtcgggag tacggcagaa cggttcggga caaatggatt tatttgagga acgccatttg
    10201 aaaacggtag atgatttcag gcacgaatat attattgcag atacgccgga agcccgttcc
    10261 gccatggcag ccgagctgga aaagtatgat tcctggtgct tcgacacgga aacgacgggc
    10321 ctgaatcccc tcatggacaa cctgctgggc gtcgcctttt gcgctgaacc gcacaaggca
    10381 tggtacatgc ctgtctccgg tccggcggat ctggaagcag tcaggccgct cctggaaggc
    10441 cccgcagaga agatagggca tcatctgaaa tttgacctgg aggttttgcg agccaacggc
    10501 atccatgtca aaggcccctt tttcgataca ttgctggctc atgccctgat cgctccaggc
    10561 atgaagcacg gaatggacgt tctggcggaa aatttgctgc aatattccac gattaaactg
    10621 aaggacattg ccgctccagg agcaaaaaaa cgggaactgg acaccagcgg cgttcccgtg
    10681 gaagtaatgg gcaaatattc ggcggaggat gcggatatca ccctccagct ttccgccgtc
    10741 ctgaaaaggc aggtcaagga aagcggcatg gaaaaactgt tccgtaccgt ggaactgccc
    10801 ctgcttcccg tgctggcgga catggagttt tccggcatcc gcgtgcttcc ggaatccctg
    10861 gagaaggctt ccgtcaaggt aggagccatc attgacggcc tacgggaaag aattgaagaa
    10921 gccgcaggcc atcccctgaa tctgaattcc cccaagcagc tcggagattt cctgttcgga
    10981 gaactggagc tggtgaagaa gcccaagaag acgaagacgg gccagttcgt gacggatgaa
    11041 gacacccttt ccgctctggc cccccagcat cccattgtag cggatatcct ggcttaccgg
    11101 gagaatatga agctgaagag cacatatctg gatgcactgc ccaggtatat ctgcccgcgg
    11161 gatggacgca tccataccca attccaccag atgctgaccg caaccgggag gcttgcctcc
    11221 caggatccca atcttcagaa tattccggta aggacggaac aggggcgcct gatccgcacc
    11281 gcctttgtcc ccgcctcaga ggaatacacc atgctgtctg cggattattc ccagattgaa
    11341 ctgcgcatca tggcggctct ttcaggagac cccgccatgt gcggagcatt cagggaggga
    11401 cgggacatcc atacggaaac ggccgcccgt gtgtacggca ttccccgcga ccaggtggac
    11461 gccgttatgc gccgtgcggc caaaacggtg aatttcggca ttatttacgg catttccgcc
    11521 ttcggtctct cccagaggct gggctgcccc cgcggagaag ccgccacttt gattgaaaac
    11581 tattttaccc agttccccat agttaaatcc ttcatggaag acctggttca caaagcggaa
    11641 caagccggtt acgcggaaac gctattgggg cgacggagga tgattccgga aatcaattcc
    11701 gccaacaaga ccattaagtc cgccgcggaa cgtactgcca tcaacacccc catccagggc
    11761 acggcggcgg atatgattaa aatagccatg atccatgtgg acaaattgct gaaaggcacc
    11821 agatcccggc ttatcctcca aattcatgat gaattgctgg tggacctgca cagggatgag
    11881 ttggctctca ttcccaagat agaggaagcc atggtcagcg cgctgcccct gcccaacggc
    11941 gttcccattc tggtggaagc caggacggga ggcaactggc tggaagccca ttaaaaaata
    12001 ttttttcaaa agactcccac gcgttccgca ttccagaatt cccgcgcctt cccgatgccg
    12061 tattttccct tccggccgta tcggtttcca gtttgtggac gagttgtccc tccaacggga
    12121 aatttttgaa aagaatgatt cccccgcggc agaaaaatgg gtgggaaagg atatcccttt
    12181 gaaaaaatga attaaagaat cagagcgtcc cctgactgga attccggaac ggtcaccagg
    12241 gctctgcctt cttttaacgc cactacctgc accttgcgtt cctgcattcc tgacggcgtt
    12301 tttacctgga tggagctgcc gtccctgacg gcggaagcag gaatccacgc agaaacggga
    12361 acttgaacgt tgatggctac ttgaagagcc atgttgacgg gagccacttg ccactcggaa
    12421 acatgggagg cattcggctc cagggaggca atcaacacgg cggaattgct tttggaatcc
    12481 agcgccgcat ccaccgccgt aacgcggccc agccactgca gccgttttcc attattcagc
    12541 acgcaagagg cggtcagagc cgtattcacc ttgccctgct catccctggg caatgcggaa
    12601 aattcttcca gcggaacggg aatccgtatc atccgctcct gaagaggaat tactttcccc
    12661 accggctgcc cggcgaccac gcgggcaccg gctcccacag agcattccac cacctggcag
    12721 gtatagggag cttccagcac cgtatcccgg agggcctgtt ctgcttccgc cacatacgcc
    12781 tccgcggcgt tcagcctggc gtttacggct ctgcgctgcg gcacccggag caccagttcg
    12841 gattcctttc cgcttttgga gacggagctg tatgtctttt tgattgcttt cagggcttcc
    12901 gcctgctccg tagaaacatc caggcgggcc tgttccagtt ccgcctgagc gttcgccaga
    12961 cgggcgcggt attcaaattt ttccaattcc atcagggggg tccccttcag gacaatttcc
    13021 cccacgttaa acaggggatg cactttgtcc acctttccgg aaacgctggg agaaagcatc
    13081 gtcaggtagg aggcctttac gatgccgggc gcggaaatgg aaagagtgtt cgtctgcagc
    13141 cggaggggtg tctccgttcc tgcaggcgcg cagcccctgt ttccggagga ggaaaaagag
    13201 acggtcggct ccgttacaat agccgtgcct atcagaagcg ccacaatccc caggccgatg
    13261 cctatggacc aggacgttcc ctttttttga ggggcctcgc tcatcaacag ctccggggga
    13321 aacgcgtccc gttatttggg atattccgtc tccatgatgt gggaataggc ctccgccgtt
    13381 tccacagcca cggtttcaat attcttcatc aactgggcaa actgggcttc cgtgaggtcc
    13441 atgccttcca gaacgaagga ctggcggtaa taaacatacc cgctgttggg gtccagctga
    13501 aaggcgccgg ccgtctggtc cgcattcagt tcgctgagga taatgctgac ttccacacgg
    13561 cggttttcag gaaccttggc ggcaaaaagc acgtcaatga ccagcgtctc cggacgttca
    13621 atcatggtgt acaggaggct caccggggtg aacgtatcac aggggacgaa tgcgatgcct
    13681 gtcttaatgt tgtggtcatc agcggaagta atccattccg tttcttcccc tctctttctg
    13741 agaacggtgt acacacggtc caaaaggctg agatgctggt tttccatatt gaaaattgtt
    13801 taacaggggg ttgcttgcaa aggcgcatgc aacgtacgcg cccgtttact gaattcaagc
    13861 ttactggcat ccttctgatt cgtcaagctc tcctgacata aagaaattga aaaaacacgt
    13921 atgcggacgg cataaaaaat acagacgccg tccaaaaggg cggcgtctgg aaaaaacgcg
    13981 tgcgccggga gttccggctt tttttcgagg ttccttttac aggatatccc cgggttggta
    14041 gcccgccccg tccggaaacc tgtccgtcac ttccttcacc atggagcgga atgcttccac
    14101 ctgggcgtca gccatgcccg tagcggagga attggaatca aaaatgcgct gaagatccgc
    14161 ccggtctatt cccaggcgcc cgtcttccgc caggcggtcc agcaaattgt tttccatgat
    14221 tttcccggct cgcaggtcat tggagacagc cacggcatgt tccttgatca cctcatgggc
    14281 ggtctcccgg ccaatgccgc gcttgacggc ctccatcagg atggtggttg tcatcaggaa
    14341 aggcaggtag cggcgcaatt ccgcagccac cacggcctca tacacgccca tctggttcaa
    14401 cacggtcagg aaggtttcaa acaggccgtc cgcagcgtaa aaggaatcag gcatgactac
    14461 gcggcgcacc acggagcagg aaacgtcccc ttcattccac tgatccccgg ccagcccgga
    14521 aatcatcatc aggaagcctt tcaaaatgac gtggaagccg ttgacgcgct cgcaggagcg
    14581 ggcgttcatc ttgtgcggca tggcgctgga gccagtctgc cccttggcga acccctccgt
    14641 ggccagttca tggccggcca tcaggcgcag ggtcttggcg aaggaggagc agccggaggt
    14701 gagttccacc agcccggaaa ccacggaaaa gtccagagaa cgcggatata cctgccccac
    14761 attcatccac ttggcaggga tgcccaggtg ggcgcacacg cggtcttcca attccagcac
    14821 gcggccggca tcctgcccga agagggaaag ctggtcaagc tgggttccaa cggcgccctt
    14881 cagtccgcgc acgctgtaac ggtccatgag ggaaatccag gcccccaggc cgtgcacgat
    14941 gtcttccccg aacatggcga cgcgcttgcc catggtcgtg gtctgcgcgg ctacattgtg
    15001 cgtgcgtccg gcaatggtca cgtgcttcca ttgttcagcc agggcgctca tgcggttcaa
    15061 cacggccacg gccttgtccc ggatgatttg catggacttc caaatctgga gctgttccac
    15121 attttccgtc aggtcccggg aggtcattcc cttgtggata tgctcgcatc cggccagctc
    15181 gcaaaattct tctatgcgcg ccttcacatc gtggcgcgtc acgcgttcgc gtgtgtcaat
    15241 ggagggaagg ttcacctggt ctttcacccg ttcataggcc tcaatcaccc cgtccggaat
    15301 atccagcccc agatccttct gggctttcat cacggcaatc cagaattcgc gttccagaat
    15361 gatgcggccc tctgcggacc agatggattt catggcggcg gaggcgtacc tttcagccag
    15421 gacattggga atcacgctca tatcaaaatg tgtatatgcg ggggttatca aaaacggtga
    15481 gccgccatga tcatgacgga agggaaaaaa gcaagcactt tcccgcagct tcacggatta
    15541 gggacgccgg ggaattccgc cggccggcaa agcctccctt ccatgcccct ggtccagcat
    15601 tgcgcctccc cgccaataaa aatttcaaga acaggcaaaa attcttattc ccgaataacg
    15661 gaatcgcaac acctccgctt accgccatgt tccctcattc ccttggcggc ggctccaggc
    15721 cgtaccgggc ataaccggag acgatttcct gaaggatggc ggaaattttt tccagggaac
    15781 ggacggggac gtattcatac tttccatgga agttgtggcc tccggcgcac aggttggggc
    15841 acggcagccc catgaaggag aggcgggctc cgtccgtgcc tccgcgcaca gggacgattt
    15901 ccggcctcac tcccacggct tccatggccc tgcgggcatg ttccaccagg tgcatgtgcg
    15961 gaagtatctt ttccttcatg ttgtaatagg aatccgtaat ttccgccttt accgtcccct
    16021 ccccgtattt acggttcagc agggccgcac attcctgcat gaattccttc ttgcgctcaa
    16081 aggcctccct gtcatgatcc ctgatgaggt actccgcagc tgcccgttcc acatctcccc
    16141 ggagtgaatc caaatgataa aagccctcgt acccttccgt aaaagcggga ttctggaacg
    16201 cgggcagcat tccctggaat tccatgagaa ccaggcaggc attcaacatc ctgcccttgg
    16261 cgctcccggg atgaatggaa gagccctgca ccgtcaccac tgcggaagcc gcattgaaat
    16321 tttcgtattc aatttccccc agggcgcctc cgtccacggt ataggcgaaa tccgcaccaa
    16381 ttcgcgccac gtcaaaggca tccgttccgc gtcctatttc ctcatccggc gtgaaggcga
    16441 tccgtatctc gccgtgaggc cgttcggggt tcagcaggaa ggaggctgcc atatccatga
    16501 tttccgccac accggccttg tcgtccgcgc caagcagggt agttccatcc gtcacgacca
    16561 ggtcctggcc caaataatcc gccagttcgg gaaaaacggc gggggagagc accattccct
    16621 gttccggatt gagcaggatg tctccgccgt cgtacgagcg cacgacgcgg ggcttcactc
    16681 cgctgccgga gactcccgga gccgtatcca catgggccac ccagccgatg acgggaacat
    16741 tcccttccac gttggaaggg atggaggcgt aaacaattcc ggaatcatca tccagttccg
    16801 cctgcgctcc catgtccttc aattccccgg ccaacagcct ggccagctcc gtctggccgg
    16861 gggtggatgg cacggtggag gaatccgcgt ctgactggga gcccacggag acatacttca
    16921 aaaaacggtc taaaacggag atgttcatgg gaaaaatcaa ggattggcaa gagtctacca
    16981 cccgcccccg cgaacacaag cacaagacac ggtttattct tgcgattcag gacggtgtgc
    17041 cgtatgtaaa caaagcttct ccccatgatg actcatctcc cccttggcat tacccttgct
    17101 ttgtccctga cctgcggctc catgcccctt ctggcccagg ggacgtacat gcccggcccc
    17161 atgcccgcca ccgccggaac aaagcaggat ccaacggaca tgtacctgga ggcgttgagg
    17221 ctggtaaccc aagccgcagg actggtggaa aaacgggatt atataggagc catccgcctg
    17281 acgcagcagg cggaagacaa gttcggacgc ctggctaagt cttatcccca atggcgcccc
    17341 aacctgctgc aaacgcgccg tcagctcaac agggaaaatc tggaccagtg gcagaagctg
    17401 gcccagcagc aggccgccgc atcttcttca tccggcctgg agctggaacg cccggcctcc
    17461 gtgccgaagc gccccaggcc aaaacccaac atagccctcc ctcccggcta caagggcgtg
    17521 gaattcccta cccgtcccgg agaatccctg atcaatacca tccccttccg tttctcccgg
    17581 ggcggctccg gaagtggtgg agagcaatta cgaacgcatc cgcagactgc tggataaaac
    17641 caccatggag aacaatgctc tgatccaggc tctcaagcgc acccgccggg agcaggagga
    17701 gattctggca aaactggccg tggcatccgc cggagaatcc gtttaccggg atgaattgct
    17761 caaggtcaaa aagcagatgg aggatgaaag aaagaccagc aacaagctta tccagactct
    17821 caccaggaga gtggaggaac tggaacaaaa tgtcacctcc ctcagccagg acaaagcccg
    17881 ttatctggcg cagatcgccg atcttcagct gcagctcagg gagtaccagg acaagctgga
    17941 caaagtgacg gatgagaaaa acgcccttca gaaagaccgg gaccaattgg cggccctggt
    18001 ggagctcaac agcccggaca aaacaaaaaa cctgttggac cgcaacctga cgctggcggc
    18061 ccagctcaag gatgcccagg acaaaatcgc cgctctggaa acagccaaat ccgattccga
    18121 agaacagcgg aaagccaacc tgaaagcgtt ggaagaggcg cgtgaggaat ccgcagacct
    18181 gaaacagaaa ctgattgcca tccgggatga aaacatcggc taccgcaaac gcatcacaga
    18241 gttgaatacc aagctcatca atgcggatgt ggaactgtcc aagctggagg ccaatccgga
    18301 aaaaagcccg cttcttctgg aagaaaacca gctgctgcgc tccaccatcg ccaagcagct
    18361 ccgcatactt tccgtgcagg accagagccg ctccctgctc atcagcactt ataagcggct
    18421 gcatcaggaa aatccggaca cggcggaaat agcctccctg atggacaatg aagaagccat
    18481 caagctcact cctgcggaaa aacaaattgt caacgccatt gccagggata acaatatcaa
    18541 tgttcccctg acggatcagc agaaggaaaa aatcaacaag ctggcacagg ccctctctgc
    18601 ggaacgcggc aaggccgccc agcttgctcg ggatttggaa ctggcgcgca gccagatgaa
    18661 gaccaaagcg gacaaatccg ccagaaacga caagaaacag gcggaagccc tggcgcgggc
    18721 ccagaaggcc ctggaacaga aaaacattca ggtggaggaa ctcacccggc agatggaaga
    18781 actgaaaagc cgttccgccg aacaggcccg cctggccgcc atctccgccg gaaccattac
    18841 tccggaccag gaaaaagcgc tgaaccggag catggaagcc acggtacgcc ggaagctgga
    18901 ggcggaagcc ctggggcaag gcgccgcaga agccttcgcc aaaaaacgct atgccgccgc
    18961 agaacagctg taccggactt tgctggactt ccagccggct cacgttccgg ccctggtcaa
    19021 tctggggacc attctgcttc agcgcaacaa ggcggaggaa gccatcaaat ttctgaaaaa
    19081 agccacggaa ctggacgcct cctcctcccc cgcatggttc atgatggggg tggcccaata
    19141 ccgcgccggg gaagaccacc acgccatcgc ctccctgacg gaaacggtca ggctggatcc
    19201 ggccaacgct cccgccctgc tttacctggg caacctggaa accagcgccg ggaactatga
    19261 aaaagcggtg gcccactttg aaaacgccct gaaaatccag ccggaatcac cggacgcgca
    19321 cttcaacctg gcctggacct attcccggct gggccgcacg gcgcaggcac gcaaaagcta
    19381 tgatgcagcc atccgctgcg gaggcttgcc ggattccgac ctggagcttg ccattaccgg
    19441 aaccaccacc cttccccgca aaaaacaggc tgcggaccag aatcccgcct ccgccgcaaa
    19501 agaggacggc ccccagctgg ccgcggcaat aagcgatccg tctgccattc cccacgacgc
    19561 caatgaagtg cctgctcatg tggcggagga tccgaatgcg caggaaaagc cgtccgccgg
    19621 tcccaaacag gtcgtcgtca gccaccgtcc ggaaaccgaa cccatctctc tggcagccca
    19681 aatgagggaa actgcccctg cggcggcatc cgcacaacct ccggcagaag ccgcaactgc
    19741 cgcaacggaa acgcccaagc cggaaaagca ggaacctccg cgcaggaaaa gccgtttccg
    19801 aatcggttcc tgatggaatt cttccctttc ccactaaata aagccatgta tcacgtttcc
    19861 attttaggac tgggcctcat tggctcacgg attgcacgcc atatggccgg aatgggcgac
    19921 aaggtcaccg tctggaacag aactccgcgg gaagattttc cggaagcggt tcctaccccg
    19981 gcggatgcgg cacgcgcttc caaagttatc cagctctacc tgaaagacag gaatgcctgt
    20041 ctggaagtgt ttgaacaaat gagaggggct ctgacaccgg agcatatcat tctgaaccat
    20101 tcaaccattg acctggcgac cgtcggcaaa ctctccctga tgagttccgc catcggctgc
    20161 acgtatgtgg attgcccgtt caccggctcc cgtttgcccg cggaaaaagg ggaactggtt
    20221 tactacgttg gcacggattc caccacactg gaccgcatac gccccgtgct ggagcacagt
    20281 tcccgggaca tcctgcacct gggcggcatc ggagccggca ccgtcgtcaa actggtcacc
    20341 aacatggtct ccgccaccat ggtgcagagt ttgggcgaag ccctggccat ctcccaggcg
    20401 tacggcattt ccccggaaat gctgggccag gccatttccc ggaacgtcat cgcctcccct
    20461 ctggctgcat ttaaactgcc cctgatggca aagcgtgact tttccaccca tttctccctg
    20521 gacaacatga ggaaagacag tatttacgct caggaacttg ccgcaaaaaa aggaatccac
    20581 ccgcctgttg ccgccctggt ttcctccatc atggggaggc tgtgccggga aggtcgcgcg
    20641 gaagaggatt tcagctgtct ggcggaacaa ttcgactaac tttttccgtt gatgaaattc
    20701 aggggaacat gcgtctttct gttgacggct cttcttctgc tccctgccgg ccggggacag
    20761 gaacgaaagg agcctggcaa tgattcttcc gcggaagtgc ggcagacgga agaagaatca
    20821 gcatccgtcc aggatgcgga ttttattgat gacgcctcgg accgtccagt attgcagggc
    20881 agttccaatg taaaccccgt cgttccccct tcaccttcgg caaggaccgc cgccggagcg
    20941 gaggaacagc ccgccaaaag gcagccgttc accagcgttt ccacctccaa gcaattccac
    21001 gtcaccggga tggactccct gctggccggg gccatcgcca cccgggcgga tgccatacgc
    21061 accgcgctgc taaaagtcct caagcagcct gacgaatgga aaaacggcat caacatccgc
    21121 ctggtaggag agccgggaag ccccgttccg gccaaccccg tccgcatgca aaccgtcatt
    21181 gtaggcaata ccctctccta tagcattttc attcatctgg gccacggcat caaccaggac
    21241 agcctgcgcc gcgccatcgt ctccaccctg ctttatgaaa tgatgctgcg cacagtggac
    21301 ccggaaggcc tgccggacaa cgtcacgctg cccccctggc tgattgccgg gttggaacag
    21361 gctgttctgt ggcacaataa tgaagctgac cgtgccatgt attccaccct gttccagcag
    21421 tccggaatta tgactccgga agaaatcctg aattgccggg aaccggaaaa ggaactggat
    21481 gcgacctctt acgcggccta tcaaacttcc tgtggagccc tggtgctttg cctgctgaac
    21541 caggaaaacg gtccggaagg gatgaaacag cttctggacc aggctattct gggcaatgac
    21601 gatcccagaa acctcatcaa acgccatttt ccgcagctca gcctgacccc cacttccctc
    21661 cataaatggt ggacgcttca gctttcccgc atggcgacgc ctcccctgac ggaaactctc
    21721 acgattacgg aaacggaacg tcatctccag gaagccctta ccctggtcaa gtatgatccg
    21781 gatacccgca ccaccgtcac atttcccctg gatgacctgg aacaggcccg ttccctgccg
    21841 gatctgaaca ggcagctttc caacgtggct ggcagcctgc tcaacctcag caggcgttcc
    21901 tttccttcat accggcccgt cattgtagaa tatgccaagc tggtggccct gatgcaaaca
    21961 gggaaagtca acagccggga agccgtcccc aaaatcagcc agcttcggga aataagaaac
    22021 ctttccatga aaaccgcccg ccgcgtccgg gactatatgg a
//
LOCUS       DAWEMX010000028        22057 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_986_length_22057_cov_11.885556, whole genome shotgun sequence.
ACCESSION   DAWEMX010000028 DAWEMX010000000
VERSION     DAWEMX010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 22057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22057
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_986_length_22057_cov_11.885556"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1462..1471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10299..10308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17218..17227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5320 a         6719 c         5604 g         4384 t
ORIGIN      
        1 tacaaaaagg gcttccatgc tcatgacgct gttgcgctgg ccttccggca gaaaatgggg
       61 aggcttggaa ccaatcaccc cccggatgcc gcggcccgag cgggttttta ccaggacacg
      121 ctggcttaac agggtatgcg gccaccagcc gcctatcccc accaactgaa ggaaaccgtt
      181 cgacgctata ttctgcacca ggaagcctat ttcatccatg tgggcggcca gcatcacgcg
      241 ggggccggcc cctccgccgg tacagaacag gctgccggaa ccatcggaag aaaattcccc
      301 atatccttca agttcctgag ccaccaggct tctcacttca tcctcatgcc ccgaaatacc
      361 gtgggcctct gaaaacttct tcagcagatc agaatcaatc gccatgggcc gagcatgtgg
      421 aaacacctct gccaggtcaa gcccgcagcc atgccgcatg tttttttctt gaccgcccgg
      481 gcagaaccgc cggacggagc cgtgtcatgc tccacactcg ctacatggaa agaaacaaga
      541 gatttttgct agccacagtt cggtttttgg tgtaactaat cagggaattg aattgtgcta
      601 tgaaatccat ccttaacact atcacggcca tgctggctgc ggtgcttttc gtccctgcgg
      661 catcggcgca gaccaccagc aatcccagaa tgcaggtgcg ggtctccctg gaaaagctgt
      721 ccctgtatat gcgccagtcc cccaacgtcc tgacgcagga cgatccccgg ccgctgccga
      781 aaccgaagaa atgggcggat tttgaaattc ccttcaaggt ggaagccgct cctaccccca
      841 aatccggcta tattgatgcc ctgacgttca aattctacat cgcggtagtc aatccggacc
      901 gctcccgcca gtacctgaaa ctgtataagg aagtcaaata cgtcaatgtt ccggtaggag
      961 aaaacacgta cgcttccgtg tatctctccc cgtcctccgt caagcgcatt accggtgtgg
     1021 aaggaggaag aggaaaatgg gtgaagtacc agggcgtagt ggtggaatac aacggcaaga
     1081 ttgtcgccac ttattcctcc gaacgcggaa aaatggaaaa atggtggacc atccagtccc
     1141 ccagcatcgt ggaaacctct tattaccccc tgctgaacaa ggatgaaaca cctttctccg
     1201 tgttctggta cgaccgttat ccggaaatta tgaggcccaa cagccagcag gcggcttcca
     1261 gttccgtccc cgccccgttc ggtactcctg tggaaccccc ggcggacggc gaataacgtt
     1321 ccattaattc tatcatttca tcttatttaa cagaaatcac ccatggctaa ttcacgacca
     1381 atcgtcgcat taaacgtagg ttcccagaga gtttcaatgg gcgtcttttc caagacttcc
     1441 aaagacgccc ttatcctgga cnnnnnnnnn ngcgcctcgt agtgctggac ccgtcagcag
     1501 aaggtttgcg cctgacgaaa ataggggaag ccatcgccga cctcgtccag gaactcaacg
     1561 tcaaaggaag cgtcgtcaac tattccgttt ccggacaatc cgttttcatc cgcttcgtca
     1621 agcttccggc tctggacgat accgatgtgg aacagctcat ccgctttgaa gcccagcagc
     1681 acgtcccctt cccgctggac gaagtcgtgt gggactacca tctgcttccc gccaaaggtc
     1741 tggaacggga agccatcctg gttgccatca aggcggaaga cctggactcc ctgaatgatg
     1801 aaatcatctc ccacggcctt tccaccggca aagtggactg cgcgcttacc tctctgtaca
     1861 atgcgtatgt gaaccgttat ccggatgaga aagaaccggt catgctcatc gacatcggcg
     1921 ccaaatccac ggatttgatt tacagcgaac agggacgttt cttcacccgc agcatttccg
     1981 cggggggaat cttcgtaacc tccgctatcg cccgtgaatt caatattccc ttcctggaag
     2041 cggaacgctt gaaaaccacc agcgggctcg tttccatgag caacgggcaa acggaagggc
     2101 tggatcccgc cacggccaat ctggctaccg tcatccgtac ggccatgacc aggcttgcct
     2161 cagaaatcca gcgaaccacc aaccattacc gcgcccagat gagcggaagc gctcccgtca
     2221 aggcatacct gtgcggcggc ggagcttccc tgccctatac caaggaattc ctggaagata
     2281 aactgggcat tccgatctcc ttcttcaacc ccatgcacaa cgtgggcgtg ggctccggcg
     2341 tggacgtgaa caccatctcc cgcgaagcct tcatcctggg cggattgatc ggcaccgcca
     2401 ttcactccat cggcagggcc tctctcaata ttgacctgga acccaccgca gttgccaaaa
     2461 agcgggccaa ccagaaaaaa atgcccgcca tcatcgtcgg ggcagccatt gccgttcttg
     2521 gtgccgcagc ttatgccgtc acaggatata tgggagagaa aaaggcggag gaaatcctcg
     2581 ccggcgtcca gcccaccgtt acttccatca aatcggcaca atccgccctg cgccagaaag
     2641 aacaggagtt gaaaaaactg gactccaccc tggcttccta ccagcagctc acgctccagc
     2701 gctacggcta cgcggacatc atcaggcacc tgctggaaca gtcggaacac aaggattatc
     2761 cctactggtt tacggatttt gaaccgctgg cccacttcaa tccggaggat accacccaaa
     2821 tcacgggata ttccgtcatc aaggattcct tcacatcgga caaaaataca tccctggtgg
     2881 atgacatcag gacggaggcc tccgccaacg catccagcga ggatgaagcg ggcgtttaca
     2941 gcgtcaacgc catccgcctg accggattcg tccggcgcag cctgggcggc cagagaatca
     3001 tccaggacct ccaggcaaaa atagacggga acaaggaatc cctgttcact ttcaagcccg
     3061 gagatgtcaa gctggaagcc cgccagatca tggaactggg agccaaagac gccaaggtgg
     3121 acgcggcggc aggggccttt gtacccttca agctggtgct tccgttaaaa acgcccattc
     3181 ccgtgaactt taacaaataa gattacccaa cccgaacaag acatatttta tgagcaattg
     3241 gattacagac aacaagcccg ccgccatggc cgcgggcgtg gggcttctct tattcctggg
     3301 gttatccgcg acagggtaca tcgtcaattc caaacgcagt gaactggaca aaaaaatcag
     3361 catcgccgcc aaggaaatca agtccgccaa tgctgcggaa atcactccga gccgctcatc
     3421 caacgaagag ctggaaaaag aactgaaccg ctatgccaag gccgtgggca gcctggaaac
     3481 ggcctacaag cccttccttg cctcctccgc gctggtcccc accacgccca cggcattcca
     3541 gaatgaactg aaaacattca gggattccct gatctcctcc tgcaagaaaa agaacattct
     3601 cataacggac acatcctcct ggctcggctt ccaggtttac agcacccagg ctccctctgt
     3661 tcaggcggcc tccacgctgg gttttgaatt gaaagccatc aacagcctgg tcaacaaact
     3721 ggcggaatgc ggcctgtcca aattcatcaa ggtgtaccgc ccccagctcc ccattgaaac
     3781 cccggcgaac aatccggaag aatcggacga agccgaccag gctccatgga ctcccatgcc
     3841 tctggaaata gccttccagg gcgaccggga aagtgtattg aaagccatga acgccataac
     3901 cggcatgcag gactatctgt tcacggtcaa ctccatccgt atccgcaacg aacggatgat
     3961 gccccctccc atcgccaatc cggcagccgc caaacctgcc gcggcccaac ccgccacggg
     4021 tgcggcttcc ctgactccgg cggatgaggc ggctgcacct gcagccccgg ccatccagca
     4081 agtcatcaag ccttacatgg gcaaggagca ggtctttgtc caggtctccc tgaatctggt
     4141 ccacttcaac cagcccaagg ctcaggaacc gtctgaagat taaccaaggc aacaccaacc
     4201 gcagcaaata ctttcaacat gtctgaaaaa caaaattacg acaaaatcct gttggcatcc
     4261 ggaatcgtcc tgggcctggg agtggccgcc tatggtacgc tgacctttct gggactgaat
     4321 gacaaataca aattcaccac gcaggtttct gaaaaagcca tcgaaccgcc tcccggcatc
     4381 aagaaagccg cagaagtagg ccaggagctt tccgcctccc acgaactcaa gccgattgcc
     4441 caggaaacgc agaagtatgt gggcttcgtc gcccccaatc tctggattaa ggagggggga
     4501 atggaaccct ttgacatcat ctccggtccg cctatccacg gcaacatccc gaacaaatgg
     4561 ttcctggaca acggactgga aaatgaattc gtttactcgg acgtcctgac ccgggacccg
     4621 gacaacgatg gatttacggt gcaggaggaa tatgcggcca aaactcatcc caacgacccc
     4681 aacagccatc ctcccctggt cagcaaactt tttgtcgacg aaatcaagca gttcggcttc
     4741 tacctggctt tcacccaggc ggatggcaat gattttacct ttaagggcat gaaccgcgcc
     4801 aaacaggaaa tctggaaaaa catcgtccag accaacggca agttcggtac ccgtaagaat
     4861 acaaaagacg agcccaggtt cgaacttgtc agcgttgtca aaaaggaatt caagaatccc
     4921 agcctggaca tggtggaaac ggacgaggaa gccgtcgtca aggatttgaa gccgaccaaa
     4981 aacggacaaa cctacaccat caggcgcgga acaaaatacg tcattccaat cattgataag
     5041 aaagtaaacc ttactatcac ggccggcccc gaacgggata ccagctttga agtagaggaa
     5101 ggttccgact tccggattcc gggagacgcc aaacagatct acacgctgaa aaccgttgat
     5161 aatgctacac agaccgtaac cattgccaac aaaaccactg gagaacaaac aacactgagc
     5221 aagaaaaaat agagccggat ttaaactttt ctgaaaaaaa agcttcacaa atccgaaaaa
     5281 catgtaaaaa gaagaaccta actatggacc acgcaccact ttatcaaccg aagcgttccc
     5341 tgatcgcgct gatggccatt gctgcctcct gcccgtttgc gcaggctggt gatggcggcg
     5401 ccgtcggaac ctccagtgcc ttgggatcgt cctacgccgg accgggcagt taccaatacc
     5461 agtccagtgc agcgcgcaca gccatggccc gccgcgaagc gcaaacccag gaagccatgc
     5521 agctccttgc ggaaggccgc aacctgtacc gcgaaggcaa gtacaaggaa gccttggaca
     5581 aatacaacgc cgcttacaac atgctgcctt ctgcgccgat caacgaccag cgcaaggaag
     5641 ctatcgccaa ccatattggc gacgccagca tcgccgttgc tcaggaatac atcaaagtgg
     5701 ggcgctatga cgaagccgac aagcttctgc aggatgccat caagctcaat ccccggagcg
     5761 ccaagcttgc caagcagacg ctcgaataca tgaaggaccc gattcgcacg aatccggcgc
     5821 tcacccccga acacgtgaaa aacgtggaaa aggtgaacac cctccttcac atggcctatg
     5881 gttactatga tctcggcgac tacgacaaag ccattgccga attcaacaag gttctctcca
     5941 tcgacccgta caacgtggcg gcccgccgcg gacaggaaac ggtcaaccgc cgcagaatgg
     6001 cttactatgc agccgcttac gacgaaaccc gcagtaccat gctggcggaa gtggacaaga
     6061 tgtgggaacg ccccatcccg atggaagtcc cgaccggagc cgacggcact gacaacgcac
     6121 cgatcacgga catcaacggc gccacggcca atctgatgaa gctcaagagc atcatcatcc
     6181 cctccgtctc ttttgaagac accaccgtgg aagacgccat tgactatctg cgcaaaaagt
     6241 ccattgaact ggaccgcacg gtaggcccga acggtgaacg cggcatcaac tttgtcatca
     6301 atgattccca gcccgctgcc gtcgctcccg ccgttcccgc aactgatgaa gacggctttg
     6361 gcgaagaaac cgcggaagtc acggaagctg ctccggcagc agccccgcag gaaagcatcc
     6421 gcacccgcaa aatcggccag ttgaagctga ctaatgttcc catgctggaa gtgctccgct
     6481 tcatctgcag caatgccggc ctgcgccaga aggtggaaga ctatgcagtg accatccttc
     6541 ctgccggcgg taatgacgtg gatctgtacc agcgcacctt ctccgtgccc ccgggcttcc
     6601 agtccgctct ccgcaccacc gtcggcgacg gcggcggcga agtcagtgac gacccctttg
     6661 gcggcggtgg cgaaagctcc tccggcctta agcccatgcc ctccatccgc agcctgctgc
     6721 aaaagagcgg catcagcttc ccggaaggcg ccacggcatt ccttgtcaac ggcaattcct
     6781 ccctggtcgt ccgcaacact tccggcaacc tggacctgat cgaacagctc attgaaaaca
     6841 cccgcggcga atcccagcag gtgcgcatca tgaccaagtt cgtggaagta acccaggaaa
     6901 acacggaaga actcggcttt gactggattg tcaccccgtt ctccgtaagc aatgaccgca
     6961 gcaccttcct gggcggcggc acgaactacg gcaccggttc cacttccgac agctttaccc
     7021 agtctcccgg cggcgtgacc ggctggcctg taaacagcgg cagcgacacc atcaacggcc
     7081 tcgttaccgg cggcaaccgc acgggtgact acgccatcac caagaactcc gtggacaatc
     7141 tgctgaacag caccaaccgc tctgaagcct cccagaaaaa cgccgctccc ggcatcatgt
     7201 ccctgacggg catttatgac gaaggctcct tccagatgct gatgcgcggc ctgtcccaga
     7261 aaaaaggctc tgacgtcctc accgccccca gcgtgaccgc caagtccggt gaaaccgcca
     7321 agattgaaat catccgcgaa ttctggtatc ccaccgaata cgaaccgccg gaactcccca
     7381 actccgtagg caacagcggc tataacaacg gttacggtta tgggaacaac ggtaacattg
     7441 tggacggcct gctgggcaat cagatccagc cccagatatc cagcttcccc gtcactcccg
     7501 ccactcccgg cgtgtttgaa atgaagcccg tcggcgtaac tctggaagtg gtgcctacca
     7561 ttggcgacaa caagtacatc atcgacctga acttcaagcc cagcatcgtg gaatttgaag
     7621 gcttcgtgaa ctacggcagc ccgatccagt ccaccggcgt tggttccgac ggcaagccga
     7681 tgtccctgac gctgacggaa aaccgcatcg agcagccgat cttctccaag cggtccgttg
     7741 aaacgtccct gttcatctac gacggccata ccgtggcaat cggtggtttg atcacggaaa
     7801 acgtgcagac ggtggaagac aaagtgccga tcttcggtga cctgcctctc atcggccgct
     7861 tcttccgcag caactccgac aaccacatca agaagaatct gatgatcttc gtaacgggac
     7921 agatcattga cgccacggga cagcccgtac gcggcaatgc ccttcccact gcggccgctc
     7981 cggaaagcgc ccttcccgcc tccgaaggcc tgctgcctcc catgtaggct gcgtgcataa
     8041 cgtccatatt ggagcgggtg aaagggtaac cctttcaccc gctctttata tcccagaagc
     8101 gccttacttt cccttccccc catgaaaacc ctgattgtta ccggaggaat agccaccggg
     8161 aaatctacat tcatccgtct gctgatggag gagggaggcg cgcgcctgcg cctgtttgac
     8221 tgtgatgcgg aggcgggcag attgctggat ggcggtacgc tgaaagcccc cctctccagc
     8281 gtcttcggcc cagccagcgt agactcctcc ggaaaagctg acaggcattt tctgcgggag
     8341 cttgttttcc ggaatccgga aagccgcaga acgctggaag ggatcattca ccccctgctg
     8401 catcaagaat gtcttgcgca aatgctggcg gcgcgtcaga atacggaggt ggacgggttt
     8461 gtcattgacg tgcccctgtt ttttgaaact tcggcgcgct attgccaaga tgccgtgtgc
     8521 gtggtggctg tttccagggg aacgcagcaa acccgcctgg ctatcaggaa tggattccgg
     8581 gaggatatga ttgaagccat tctggcggcc cagcggccta tcatggaaaa agtggctgcg
     8641 gccgacttcg tcatctggaa tgaaggcccc ccggacctgc tccgccagca aacccaaaga
     8701 ctttaccaac atttttttca tgactgaaga actcgacaac accccttccc tcgccggaga
     8761 tattccgcag gaaaccctcc ccaagcccct gcccgcgccg gaagaaacct ccggggaaca
     8821 gccggctgcc gctccggagg aaaatagtgg aaacgcagtc cgggaggagg aagcagcccc
     8881 tgttctggag cagattgaca tcaatgaact gcggaaacgc cccctgaacg atcttcagga
     8941 aatggcggaa gggatgccca tcaggaacgc ggcctccctc accaagtccc agctggtttt
     9001 tgaattgggg aaacagcttc tggcaaaggg ccatgaagtc gtagtttccg gggtcatgga
     9061 acaagccaag gataattacg ccatgctgag ggatccggtg aaaagtttcc gcacctctcc
     9121 ggatgatatt tatctgggcg gcaatctcat caaacccctg catctccgcg taggccagca
     9181 gatcaaggtc aggctgcgca aattgcggcc tcatgacaag tacctttctg cagcctccgt
     9241 catcagcgtg gaggacatcc ctgcggaaga ctaccgggcg cgcagcgatt ttgaacgcct
     9301 cacccccctc ttccccaagg aacgcctcct tctggaaaac aagggggtca attccgccgc
     9361 catgcgcgtg ctggacctca tgacgccctt cggcaaaggg cagcgcggcc tgattgtggc
     9421 ccccccgcgc ggaggaaaaa ccgttcttct gaaaacaatc gcccgttcca tcagggccaa
     9481 ttatccggaa gtggaactga ttgtgctgtt gctggacgaa cgtccggagg aagtaacgga
     9541 ttttgaagaa accgtggatg ctccggtatt cgcctccact tttgatgaac cttcccggcg
     9601 ccatgcccag gtttccgatc tggttatcga acgggccaaa cgcctggtgg aaatgggcag
     9661 agacgtcgtc atcctgctgg attccctcac caggctggcc cgcggctaca atgccaacca
     9721 gacgggagga cgcatcatgt ccggcggcct ggggtccaat gcattggaaa aaccgcgcaa
     9781 attcttttcc gcggcgcgca atgtggaaga aggaggcagc ctgaccatca tcgccacatg
     9841 cctggtagac acggaatcaa gaatggacga agtgattttt gaagaattca agggaacggg
     9901 caatctggaa atccgcctgg accgggaact ttccgaacgg cgcatttatc cggccatttc
     9961 cctttcccag agcggcaccc gcaatgacga caggctgtat aacgaacagg aattcgtcaa
    10021 aatcatgcaa ttgcgccgcc agctcgccat gaaaccgggc tgggaaggcc ttcagactct
    10081 cctgcaaaat atctccaaga cacagaataa cgcggaactt ctgctgacgg ggctgcggta
    10141 agattttctg cccggcctgt ttttcccccg tcctccgttc cgcccgccgg aacggaggct
    10201 tttttatcag gatatcttcc gctgttccaa gattccgcgg cagcatccgg atatcgggat
    10261 gggggcatcc tcccataaac agggggagga agagagttnn nnnnnnnnct tcacccggaa
    10321 aatacgcatg gagttgccca ccgcaagcag gcagacgccc acatccgcca tcacggcggc
    10381 ccacatccca gccagcccca gaatacccag caccatgacc agaattttga cgcccaaaat
    10441 aagaacgatg ttctgcacaa tgatgcgctc tgtggcttgg gaaagcatca tccccagcgg
    10501 aacggccaaa ggatctccct tcatcaccac tacgtcagcc gcctgaagag ccgtatcgga
    10561 ccctacgccg cccatggcaa tgccgatgtg ggcagcagcg agggaagggg cgtcattcat
    10621 accgtccccc acaaaggcaa gcagccccgt ttcaggtttc agccggcgca catgctccag
    10681 cttgccggca ggcatcagcc cgcagaatac cccgtccatc cccagcttaa gccccacttc
    10741 cgttccggcg gaagaagaat cccccgtcag catgaccagg ttggaaacgc ccagttccct
    10801 caatttccgc accgcacgct ccgctcccgg cttgacgcgg tcagacaggc ggaggcatcc
    10861 cagaagtatg ccgtccaacg ccgcataaac ggtcacgcca tggtcttctc cctcctccgt
    10921 ccttacgccg gcctcttgaa gaaaagactt tttccccacc agaaccggtc tcccttccac
    10981 gcgggctgaa acgccgccgc ccgtcacctc taccagttcc gccacgcggt ccggaaacag
    11041 agtcccttca tatgccttca caatggaacg ccccagcgga tgggaggcgg agagttccgc
    11101 atgggccgcc cagtacagca gttcctccgg ggaaacgccg tcacggggaa gcacttcatc
    11161 cacggaaaaa acgccttccg tcaaggttcc ggttttgtca aacaccaccg tcttcacctt
    11221 ccgcagggca tccagatagt tgctcccttt cagcaaaatc ccctgccgcg ccgctctccc
    11281 tattccggca aaaaagccca gcgggatgga cagtaccagc gcgcaggggc aggaaatcac
    11341 cagcaggacc agggcgcgga acagaccgtc cgcccagctt ccgcccgtca caaggggata
    11401 aagcaaaaat accagagcgg ctatggaaat caccagcggc gtataaatgc gggaaaatga
    11461 ggacagcctc ccctccagag gggatttgcg gcctgatgcg gcttccacct gttcctggac
    11521 ccgcgccagg gcggaatgcc gccagtcccg ctccgtacgc acttccagca caccctgccc
    11581 gttgatgtac cccgccagca cggggcttcc ggatccggct tccaccggca gggattcccc
    11641 ggtcatggcg gaggtatcca gcacggagaa acctcccgtt accacaccgt ccaacggaac
    11701 gcgttcgccg gggcgcactt caatcaggga gccgggcaga acctcggaag gaggaacgtc
    11761 atctacggct ccggcttttt tcacatggac ggcctcaggc ctcagactca tcagttcgct
    11821 tacggaccgg cgggagctgc ccaccgcacg ttcctgaagg tactctccca cgcggtagaa
    11881 cagcatcacc cccaccgcct cggaataatc ccccaggaac atcgccccca gggaagcaat
    11941 actcatcaga aaattttcat tgatggcgcg gccgcgcatc atccccagaa aggcggcctt
    12001 cagtacatcc cagcctatca gcaggtagga gcccacgtag gcggccatac cggccaggga
    12061 atcgccgccc gccgccagcg cactgatgaa cagggcaatg cccagcacgg cgggaacagg
    12121 cccgatggcg tctccggagc agcaacagct tccagaaccg cagcttccgg aacaacagga
    12181 agcaccttcc ggaaaatgtt cgtgggaatg ttccgtgctc atgacctgtt ccgcttgtac
    12241 gcctgcctct ccggcatggc aagccctata ttagcccaaa ctaacaaaaa gtctgcgttg
    12301 atttctgatc cttaaaccgt tccaaaatcc ccatccccat gaattcatcc atcgccatgg
    12361 ctcccgctcc cagcaaccct gcctccgctc caaatctggc aggcagaatt ttcaaatcct
    12421 ccatcaggag aggaaacaac tgtttcctga ggttctccag cagcggcttc atcaacaaat
    12481 cccctgcctt ggccacaccg cccccgatga tgaaggcgtc cggcaccagc gtatacatca
    12541 ggttcatgat caggcagccc agcatttccg ccgtatcttc ccataattga agggctatag
    12601 gacatccgga ccgggcagcc tcgtccagat gcctgggcgt gcattcatcc accgtcttga
    12661 tgattcccgc cccggcatac cgtttaaccg cctccgccgc cagttcgttg ttgccgatgt
    12721 attcctcaat ggctccccgg ctgccgaacg gtcccgtttt cccctggtaa tgaatgctgg
    12781 tctggcccag ttccgcagcg gaaagccgcc ttccccgcag catgcggtca tgcaccacga
    12841 ttcccccgcc tatccccgtt cccatcgtca ggcacaccag gctggacatg ccgcgcccgg
    12901 cgcccagctt ccattccgca taagccatgc agttggcgtc attatccagc acgacgggga
    12961 ggcccagggc ctgctccatc atctctttca ccggaatttc cctatcccag accctcacat
    13021 tggtaagctg gtaaagcact ccccgctggt aatcacacca tcccggcatt cccatgccca
    13081 tcacacaggc ggaggggaat tgtccgcgca gggtgcgggc ggacgcaatc atggcatcaa
    13141 tgattgcctg cgggctttcg aacatggcag tagggatgcg gtcggccgtc gccagaatac
    13201 gctcccccgc cgttacgccc attttgatgc tcgtgccgcc gaaatcaacg gccaaggcac
    13261 agggttctga aaagctcata gtccatactt atgccaccca ttctccggaa tcaacgtaaa
    13321 aacgcattca tccccacccc tttccgcggc atggcgcact taccgtataa aacacaataa
    13381 gaatcttcca gaaagagctt gccagaaccg cgccgcccgg tccatgataa cctccgtaat
    13441 gatggaaatg cccggttcgg acgaatggtt catgcgccaa gccatgaaag aatccagaaa
    13501 ggccctcgtg aaaggggaag tgcctgttgg agccattgtg gtgaaggatg gccgcgtgat
    13561 cggccggggc tggaaccagg tggagacgct caaggatgct acggcccatg cggagatgat
    13621 tgccctgacg gcggcccagg aggctttggg cgactggcgg ctggaaggct gcaccctgta
    13681 tgtcaccaag gagccgtgcc ccatgtgcgc aggagctatc gtccactgcc gtccggaccg
    13741 cgtcgttttc ggctgcccgg atgcaaaaac cggggctgcg gggggctgga tcaatctgct
    13801 ggattccaat ccgcccctga accataagtg cgaggtgcgt cccggcgtac tgggagacga
    13861 gtgcctgctt catctccagg agtttttccg tgcggcgcgt ctggcggcaa aagaacggaa
    13921 aaaattgtct gcgcgtcttt cttcccctcc ggatgaaaac ggggaatgaa gtccggaata
    13981 aaacactttg catttgccaa agcagcagag tcacggcaag ctagcgggac tatggaacgc
    14041 cggaacgaca atgagactgc ggaaggaacg gaacgcaaac cgtcgtggat caaggttcgt
    14101 ctgcccaacg gccgggaatt ctgggacgtc aagcagcttg tggaaggcaa aaacctcttc
    14161 accgtctgtg aagaagcaca ctgccccaac cgttatgaat gttggaacca gggtacggcc
    14221 acgttcatga ttgccggaga acggtgcacg cgcgcctgcg gattctgcgc cacccgcacg
    14281 gccaagcccg gcccgctgga tcctgaagaa ccgcagcacg tggcggaagc cgtcgtgcac
    14341 atgaagctgc gccacgccgt aattaccatg gtcacccggg atgatttgcc ggacggcgcc
    14401 gccgcgcatt tcgcccaggt aattagggcc gtcagaaaag ccagcccttc cacgatcatt
    14461 gaagtgcttg cctcggactt gaatgagaag ccctcctcca tccagacgct catggcggcc
    14521 cgtccgcata ttttcggaca caatctggag acggtggaac gcctcacgcc cattgtccgc
    14581 ttccgcgccc agtaccggcg gtccctgcgc gttctccgga tggctctgga gtgcgtggac
    14641 ggcaaagtgt ctaccaagag cggcatcatg ctgggcctgg gggaaacgga agaagagctt
    14701 ttccgctcca tggacgattt gctggagcac ggcgtcacgg tcctgacgtt ggggcaatac
    14761 ctgcgcccct cccgcaacca tcttccggtg gtcaaatacg tccatccgga tgatttcgcc
    14821 cggtatgagg aagttgcgcg ctccaaaggc ttcaggcacg tggcatccgg gccgttggtc
    14881 cgcagctcct accacgccgc caatttcacc ccggaagcgg atgttctgga agccattaac
    14941 gaagacctga gaaaggccgg cgaactttag aataaatctt gattttttat ccttcccctt
    15001 ttaccatccg ggcaattcca cgtcattgag aatctcatga gcaaaacatg ttccttcatc
    15061 aaggcaggag ccattctggg cggcacggtc atcgtgacgg cggctgtcgc ccccctgttc
    15121 ctgccgaacc agaatgtcaa gccactgacg tccgcccagg cggcggaaat taccgcacag
    15181 gccatgaatt ccaaatgtgc ggactgccac aagcccggca ctcacatttc cgaactggtc
    15241 aatactcttt ccggaggcct gctggcgcgc catatcaggg acgggcagcg cagctacaat
    15301 atggaagaac ctcccactgc cgtcaccctt tccaagctgg aacatgtgct tcaaatcaat
    15361 tccatgcccc ccacttccta caccatggtg cactggggca gcacgctcac tctccgggag
    15421 aaaaacgcca tgctccagtg gatcaaggat gagcgcctga aaattttcgg cgatatggta
    15481 ggagaggaat acgccctttc ccctcttgcc cccattccgg acgccctccc cacggatccg
    15541 gccaaagtgg ccctgggcta caagcttttt catgacgtgc gcctttccac ggacaatacc
    15601 gtttcctgcg cttcctgcca ttccctggaa aaagccggga cggacaacct gcccacttcc
    15661 accggagtcc gcagccagaa aggcggcatc aatgctccca ccgttttcaa tgccgctttc
    15721 catgccaagc aattttggga cggacgcgca gccaacctcc aggaacaagc cggcggaccg
    15781 cccctgaatc cggtggaaat ggggtacgaa catccggatg actggaagaa gatcgctgcc
    15841 aaactggacc aggacgccgc ttttgccgca gaattcaaaa aggtttaccc ccagggattc
    15901 accggagaga ccatcacgaa tgccatcgcg gaatatgaaa aaactcttat caccccgaac
    15961 agcccgtttg accgctacct gaaaggggat gaaaacgcca tcagcgagaa cgccaaaaaa
    16021 ggttacaagc ttttcctgaa gcttggttgc cagacctgcc acaccggtcc cgccatggga
    16081 ggccagtcct ttgaatacgc cgacctcaaa ggcgatttct ttgccggacg cgccaagacc
    16141 aacgacgata acggcctgat gaatttctcc aaaaaggaat cggacaggca ccgcttccgt
    16201 gttccgaccc tccgcaatgt ggaactcacc tggccgtaca tgcatgacgc ctccgcacag
    16261 actctggagg aagccattac gaaaatgtac cattaccagc tcggttacga taaactggac
    16321 aagaaggaag tgagacttct ggtggctttc ctgaagacgc ttaccggaga atacaacggc
    16381 aaacccgtcc agggcgaagt ttgccctgcc tcctgaatct gaagaaaagc tctttttcca
    16441 gccgcgccaa tgaacattca ttggcgcggc ttttttgcat ttcccttcca gttcctacgg
    16501 cagacactgc cgggaaactt ccattccccg cctcttcccc gctggccaca cttagattaa
    16561 actaatacgc aaacaattta tattaaacgc atttgacatt gattgaaaaa atatatccta
    16621 ataatatcat tgacttatca gtttgataaa actaacttct ttccggattt gattcataca
    16681 cgcaccatgc cactctccat gctcaacatc ggcgatattc gccaagtcaa caaaatccat
    16741 ggcaaggatg aaacccggcg ttttctggaa agcctgggct tcgttgccgg gagcacggtc
    16801 tccatcgttt ccgaaaacgg cggtaatttc attatcaacg tcaaaggctc caggattgcg
    16861 ctcagcaagg cgcttgccag caaaatattc gttgcctgag ttattccatc caacaaccaa
    16921 ccattattct atgaccaaac atcgtacatt gagagatgcc ctcatcgggg aaaccgtcac
    16981 cgtcggcaaa ctggcggggg aaggcccggt cagaagacgc atcatggaca tgggggttac
    17041 caagggaacg cagatattca tccgcaaagt cgcccctctg ggagatccca ttgaagttac
    17101 tgtccgtggg tacgaactct ccattcgcaa atcggaagcg gagaacatcc atatccattg
    17161 attccgcctt tattaccgca cgtttcctga ctttacagca agacgggcac ccatatannn
    17221 nnnnnnnaag acaaggaaac cattcatgag caacattacc attgccctgg caggcaaccc
    17281 caactgcggg aaaaccacgc tctttaacgc cctgacaggc gccaaccaat acgtcgggaa
    17341 ctggcccggc gtcaccgtgg aaaaaaagga aggccgcctg aaaggccata aaaatatcat
    17401 cattgaagac cttccgggca tttattccct ttccccctat accctggagg aagtcgtcag
    17461 ccggaattac ctggtcacgg accatccctc cctcatcctg aacctggttg acggcagcaa
    17521 cctggaacgc aatctttacc tcaccaccca gctttgcgaa ctgggcgttc ccgtcattat
    17581 agcgctgaac atgatggaca ttgtccggaa gcgcggcgac aggatcgaca cggaaaaact
    17641 tgcacgcaac ctcggttgcc aggtgctgga aatcagcgct ctcaagggga ccggcatccg
    17701 tgaactgata gatgccgtgg taacgacgac tgcgtcggac gactcccgga ccccgcaggc
    17761 cgtccacttt ccggaccatc tggaaaaagc catcggagaa attggggaaa tcatcaccgg
    17821 aaaatgcctg gatcagcaga aaaaatggtt tgccgttaaa gttttcgaaa gagaccagaa
    17881 agtcgcggac cagctcgcgc tcaatcccga ggaacaatcc cgcatcaacg ggattattga
    17941 aaccatggaa cgggaaatgg atgacgacag tgaatccatt atcaccaatg aacgctataa
    18001 ttccatttcc cgcatcatca aagacgccgt cgttaaagcc agccccggca gcctgacggg
    18061 cagcgacagg attgatcgca tcatcaccaa ccgctggctg ggcctcccca tttttgccgt
    18121 cgttatgtgg gctgtatatt acatctccat tcagacgata ggcgcatggg gcacggactg
    18181 ggccaatgat actttcttcg gggaatgggt tccggaatgg gtaggaagcc tgctggaaaa
    18241 cgccggctgc gccccatggc tggaaagcct gatccaggac ggcgtgattg ccggcgtggg
    18301 agccgttctg ggattcctcc cgcaaatggc ggttctgttc ctgtgcctgg gcatattgga
    18361 ggattgcggc tacatggccc gcgtagcgtt catcatggac cgcattttcc gcaaattcgg
    18421 cctttccggg aaatccttta ttcccatgct cgtttccatg gggtgcggcg ttcctggagt
    18481 catggcgacc cgcaccattg agaacgaaaa ggacaggcgc atgactatca tgaccaccac
    18541 gttcatcccc tgcggggcca aaactcccat cattgccctg attgccggag ccttcttccc
    18601 ggaaaattcc tgggtggctc cgggggccta tttcatcggg gtgggcgcca tcatcctttc
    18661 cggccttatt ctgaaaaaga ctgccatgtt cgccggggat cccactccct tcgtcatgga
    18721 acttcccatt taccacatcc cggcctggaa gaacgtcatc attcatgcat gggaccgctg
    18781 caaggctttc gtacaaaaag ccgggaccgt catctttgtg tccagcgccc tgatctggtt
    18841 cctctccagc tataactgga aggtggattc cgtagagcag aatgacagca tgctggcctc
    18901 catcggcaat gtggccgcgc ctgtcttcca acctctgggc tggggtgaat ggaaacccac
    18961 catggctacc attaccggat tgattgccaa ggaaaacgtg gtaggcacct tcggcgtcct
    19021 gtatgcggac ggcagttccg gggaagatga agcggaaaaa acggaggact cccccgaaac
    19081 gcaggaaatg gctcccgccg gccgggagct ccaggcatcc ctggaggaag gttccggaga
    19141 atccgctgct gaagcggaag aagaggaaga tgaagatgcg gaaatcaggg aaaccgggga
    19201 ccgcctggtc caagcgggag ccttcacctt cctgagcgct ttctccttca tgattttcaa
    19261 tcttctctgc gccccctgct tcgcggccat gggcgctatc agaagggaaa tgaacagcgc
    19321 caaatggacc ttcttcgcca tcggctacat gtgcgccctg gcgtactgcc tggccttcct
    19381 gatttaccag ctgggagcat ggctttatgc ggaagcttcc ttcggcatcg ggcagggcat
    19441 tgctctattg cttctgctgg ggttcgttta cctggtggca aagaaacctt atacagcctc
    19501 caaataactt ctaaaccaac tccttaactc tactgaccaa tatgggaaca tatatcatcg
    19561 ccacccttct tttcctggcc ctggcttacg cgttatacaa aattttcagc aagcgcggca
    19621 agtgctgcgg caactgcgga tgttgcggca acgacagcga cggctgttgc ggtggcggcg
    19681 gaaattgctg cggcggccac catcacgacc acgaaaaaca ggattaaatc ccggccccgg
    19741 ttatcccatt cagaacctgc aaaccatttt ctgcggtttg caggttcttc tgctacggcg
    19801 cctgccgcag ccagcctcta ccctgtcact caatccaacc gtgctgatat gccgccatgg
    19861 ccgcaattac cagcgcaatg aacaggaaga tgaaccacat cacattgtgg cgatggcggt
    19921 attccacggc catcagaggt tcccattcct tttccacaaa ggaaaaatcg tccccattgg
    19981 caaaacgatc gtaaagcatt tccagctcct ttcccgtcac cccttccggg gcggcgaata
    20041 ccggcgggca gcaggaatcc gtcttgaata caatttcgca atggtacacg ccatccgctt
    20101 ccttataaga ctggaggcac gcgccgtcgc gggcgcacac cacgtggccg gtcgttcctt
    20161 cctgcaagcg gcgtagagca gtatcacggt cggtcacttc atcatttgaa atttcccatt
    20221 gaaccttact cattctgtca gtttaagata attcccgttt aagtcaatat ggttttaaac
    20281 gcttcggccc ccggaaacgt tgcggctccc atccatcttc cggcgtggca aaaagccttc
    20341 ttttccgttt tttccgggaa cggagacagg ggtgggaaag cccattttca ggcggctttt
    20401 atttttcccc gggataaaaa ttctacggaa cagccttggg gaagcctcct accttcaaca
    20461 gtcagtattt gacggtagat aaggaaaggc tgaaggccat tccatgaccc ggcatacatt
    20521 gccggtccaa gctccgccag caggcatcaa taaaggaggg taaatggctc cgtaatcaaa
    20581 taagcaacgc cgcatcctct acctgatcga gaatgcggcg ttgccgccag gtcatgcagc
    20641 ccccttctat atctttctca cgcaggattc cgctcactgc atggagacag acaaggaaga
    20701 tttaatgtca ttatgttttc atgaaactcc ggccatgcct gatttaagag aaaaggcggc
    20761 catgtccagg atcagctgat cagaaagaaa agactgcctg aacgccccag agaatgtttt
    20821 cgctggaagt gctgtaagcc ggattcgcga tgtactggaa gtgaggaaca actttgaaat
    20881 aatcattcac ctggaagctg tacatgactt ccagcacctg ttcgcggtta tgctcagcgc
    20941 gctctccgtc acagggggtc tgccccttga acacaccgta ggaaatgccc aggaagtcat
    21001 cttcacggga agggaagagc ttaatgttgg cgcccacgga gaattcagcg gagttgctca
    21061 agtattgttt ggcggcaaat ccggcgcgag agtaaatggt cagaggatcg cagggagtat
    21121 attcgatgct cgcgacaagc ccggcatttt ggcggcggcg ttccccggtc ccgtcgtcca
    21181 tgtccacatc cgtgctggta tagaacggat tgatgcggag agtcgcagcg ccgtcggcaa
    21241 agatatggcc gtattcgcca accacggcat aaccatcgca ataatcggaa ttaaaaggat
    21301 tgtatccgga atcgcatccc gtgcgggaaa cggcaaccat catgtaatta ttgcggttga
    21361 gttcaacttg cagaataccg ccaatattgc tgtccaccaa aggcaggata gtggagttca
    21421 caaacccgtc attggtgaaa gaggagaagg agtcattggc aatgctgaca gcatcaaagt
    21481 agttggtgag attcaccata cctgcgatga ggcaagcgcg cttccctgca aagtattgca
    21541 tcagcgccac ttcagggaat attccttcat gaggccccat cgcgtcagca tgcaagccgg
    21601 aagccgtggc ataggcttca gtgaaaaacg tatcgctctg tgcggattca cgatccagcc
    21661 cccatgaacc ggaaaattcc acacgcaacc aggttccccc gttggccttg tcttcaataa
    21721 ggcgctgatt aagctgggca tgaagaagag ccaggttgtt atggcgctta acatcggaaa
    21781 ccttttccgt attccaaagt ccgtaggcca tatccacacc ccattgggtg ccgtatttct
    21841 cattggcctt caccttgtag gcaggatcgc cttcttccgg agtgaacata ttgatgccct
    21901 gcaaaatatc accaacagca ggggtgctgt cttgagcgat tacaggaagc gccatgaggc
    21961 tcgtcaacgc cattgctatt atgttatgct tcttcataga aaacggttag tcttatcgtt
    22021 ggatgtttgt ggtataatac ttatatgatt gatccag
//
LOCUS       DAWEMX010000029        21954 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_995_length_21954_cov_15.468560, whole genome shotgun sequence.
ACCESSION   DAWEMX010000029 DAWEMX010000000
VERSION     DAWEMX010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 21954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21954
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_995_length_21954_cov_15.468560"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5300 a         6587 c         5824 g         4243 t
ORIGIN      
        1 gtcccaacat ctgaatcccc cgcatgggga gcggactcca cctttaataa caatgagccc
       61 gccaatgagt attcagttac tgggagccaa tccgtcaacc ttgatgtaaa ttcaggaaac
      121 aacaattatt ccacaggact ttatatcggg gcaggctcct cgtttaccat taatcagaat
      181 gcgaacggcg gctgtaccat caatttgaac ggggcatttg ccggagaagg aaatttaacg
      241 ttggtagcgg ctaacggcaa tgccggctat gcctccaagt ttgtgcttgg ttcccaagaa
      301 agcagttttt ccgggaacat tatcctttcc cagaagggga cgcagcccgg cggtgccatt
      361 ctccagatta cgggaacggc tctcgccaac gcaacggtag atttgtcagg ctcaatcaat
      421 caaagttcca gcgccctgac gttgcaaatt tccaatgccg cctctctggc cggtttaaat
      481 gatgccgacg gcttcagcgg aacacataag gggcgcgtcc agtcggctaa ttcctccaga
      541 gcgaatctta ccctgacggg taacggaaat tacacgtatg ggggaagtat cggggcaacc
      601 acacagcatt ccggagtaaa cggcaacact actcccacgg gaggaataaa tctgattatg
      661 gccggaacag gtacgcaaaa cctgacggga accgtcatca acgcgaatat cacggcccaa
      721 ggtggcatct tgaaaatcaa taattcttcc ctggcctatt ccggaataat caccatggcg
      781 ggaggaacgc tggatttcac ctccgccact ctgggggcaa atgccgttct gaacatgaac
      841 ggcaccggcg tattgaaaaa cgcagccatt gacggagcca agctaaccta tacggaatcc
      901 ggatcctcct ttaccaagga aaatgtaact ttcacttcag gcaccattga catcggcggc
      961 gcgcttgaca gcctggtgga gggagaacag ggatacacgt ttgatttagg aagcaacctc
     1021 ggcacgaatt ttaccgttac aggcttagat acagggcaat acagaataga aggcagcgtg
     1081 ctgacgatag aagacggggc gatatccagc gtgacgtggg tatctgcggg agaaggaggg
     1141 gaattggaag aaacggtcaa gaacgccttt acgctggctc ttggggaggg gagtgcggcc
     1201 aacgtcagcc tgggatatct gaacgggacc ctgacgacca gcggggacaa agtctatcaa
     1261 atcaccaaca ccggcggaac caaaatcaac ctgtccgggg tatacaacag cggggcgacc
     1321 ctgccgtccg gcaacctcaa ctaccagggg gacatctgga tggacatcaa cggaggagca
     1381 ttcggcatca tcgccggagg tgtcaccaat gaatggggca ccaacctcca gaccagcacg
     1441 ctgactgggg acacccatgt gcagctctct ggaaacgcca cggcggaaca cgtcatcggc
     1501 gggaacaaca agggagcaag cactaccctg acgggaaaca ccaacgttac cgtcaaagat
     1561 aacgccatcg tggcgggagc catcatcgga ggaagcacct cctcccacaa tgccgtcacc
     1621 accatcacag gaaacacgag cgtcctggtc accaacatcc agcacagcaa cagcgcgacc
     1681 gtcaacctgg gagacttcgg caacgtcaca gcccaaaact tcatcacggg cggaagcgcg
     1741 tggacggcca accagacttc aggaacgacc atccagggca gcacctcggt aacgattaat
     1801 gtaggcgatg cggaactctc cggcacggaa gggcacaaca acttcgtcaa aaacatctat
     1861 ggaggcagtt acgcaaacac aaaatccgag ggcaacggag ccgtacaaaa agtggaagga
     1921 aactcaagcg tctccatcag cggaaaagaa ggaatcacct tcacgggaga catcatgggc
     1981 ggctccttct ggaactgggg caacggcacg acgctgacca ccaacggaaa caccagcgtc
     2041 tccattgacg gaggttccac cttcacgggc aaaatcgtgg gcggctcctg gagaggaagc
     2101 acgtggacgg cggaagatcc cacagccctg cccgtcagca ttggcggcaa catcaccgtc
     2161 acccttggac agggcaccta cttgggagac atctacgggg cgggcaactg tggcacggta
     2221 ggaggcgaag tccacgtctc gctgacggga ggaagcatct ttggggagga aggagaagaa
     2281 agcggcatca ccatcggcgg aagcgcagga gcggcggtag aaggaaacag gacgctggaa
     2341 ctcaagggaa cctttggaac cggggacttc caaaacgtca ccttcacccg gtttgatgaa
     2401 atcaatatcg cgcaggaagg aacctcggcc accatctacg cgctgaccga cagcccgtcc
     2461 ctgaccaaaa cgggagcggg aaccctgaca ctcggggcgg acgcggcagg agcggaaacc
     2521 atccttgacg gaaccacgga aggaattatc atctcggaag gcagcctgaa cctctccggc
     2581 gccggcggca gccatatgaa aggaacgtgg aacatcgcct ccggctcccg tctcaccgga
     2641 gtcagcggaa ccgtaaccgt aggagaagga ggcctggacg gactgaccat cgccctggga
     2701 acggaaaaca tcggccagga cacgcaggcg tctggcgccg tcatcagcaa cggcagcgga
     2761 acgggaagcg ccccgaacct gtccataaag ggggaagggc tgacgctgga cctgagcaac
     2821 gacgccgtcg tcaaccttct gctggaccac aaaaccgacg actcctccag ctacctgacc
     2881 ctgaccagcg gaacgctgac cgtcggcgac ctgggcgaca tcgccttcac taccgacctt
     2941 ctgtccaact acggcatccg ggtcaccgga acggacggag gcagcctggt gctcagcgga
     3001 gcggccaccg gcctctaccg cgtgcaggag gaaggaggaa acgcccatga agtcaactcc
     3061 tatcaaacgc tctccggcta cgccggcgtc gtcatcggcg gcggccagac cctgacggtc
     3121 aacctggccg gagccccggg cgaatccgac ggacagggag ctaaaatcaa caacctgatg
     3181 ggagccacgg gcagcagcct ggtcgtcaac aacaccggag acggcacggc cgtcgtcatc
     3241 ctcaacaaca aacaaatgac gacgggagaa gacgacatcg acccggccgg ccaggacacc
     3301 gtcatgggcg gcagcatcac gggaggcaac aacgtcgcct tcatcaagga aggaaccggg
     3361 acgctgacgg tcggaggaac gatggacgtg gaaaccctcg ccctgcgtga aggaaacatc
     3421 gtcctcaacg gcgcgtcaaa caccctggac accctcactc tggaaggagg cggcctgacc
     3481 attaacggca acgccgaagt cggaaccatc accggcacgg aagccggagg ctccctgacc
     3541 atccagggaa ctctcaacct gaccggaaca ggcgaaataa acgacggctc catcaccgga
     3601 accggcaccc tccgcatcca ggaaggagcg gaactggccc tgggcggaga agctcggctg
     3661 gacggaacct cagtcaccgc cgacggcacg ctggccctct ccggaacgga atccggcacc
     3721 atcagcagcc tctccggcag cggcaccctg tccatgaacg gaggccgcct ctccatctcc
     3781 tcggcaacaa cctcctccgg aaccttctcc ggcacgctgg caggcagcgg caccctggac
     3841 atctccgggc aagccaccca atacctgcaa accggcaata aggactacga cctcgccgtc
     3901 cgtgacggag gcgtcctggt cctgaaaggc accgcggacg cccccacgct gaactacaac
     3961 agcatcaccg ccggaaacaa cggcaccctg cgtattgaag ccaccgggga cgcccagggc
     4021 agcgccaaca ccacgctcaa cgtggaaaac atcaccttcc agaacggctc cacgacggaa
     4081 ctgatctaca acttcaacca ggacgccccc tttggcgccc ccatgctgac ggcgggcacc
     4141 atcaccgtgc aggacggagc cggcttcctc ctctccaaca tggaaggaaa cgccgccatg
     4201 aacgcgggga gcgacctcca tgacgtcgtc ctgatgagcg ctaccggcag catcagcggc
     4261 ctggaagacg ggcagagcct cgccgcccgg atatccggcc tcttcgccgt ctactaccag
     4321 gacgccacac tgagccgcga cggaaacgac atcctgctca atgccacact ccggcaggaa
     4381 aacctcttcg cctccgcggc cgacacgtgg aactccgccg ccggagccag cctgctctgg
     4441 gaagcccgca aaaacctgga ccccgactcc cagctggccc aattcatgaa cggcgtcagc
     4501 accatgatca atgacggcaa cctctccgga gcctcccgcg ccatggcggc ggcggcaggg
     4561 agcacggtca acgcgctggg gacggcgcag agggacgccc tgcgcgacca gatgggctgg
     4621 atcaggaacc ggaccaccct catgggcgtc aacccggcct acgtcaacga cgacctcccc
     4681 cgcttccaca tgtggatgga aggcacgggc tcctacgcca aactggacac ccgcggggat
     4741 gaaagcggct accagctcac cacctgggga ggcaccgtgg gcgtggacgc ggacctcagc
     4801 gaccgcctca cggtgggagc ggccttcacg gccggctacg gcgacctgac ggccggcgcg
     4861 gcggacagcg ccgacgggca cctggacagc tactacgcca gcctcttcgg ccgctaccag
     4921 gacaggcgct gggcgcacac gctcatcctg acgggagggt ggaacgacgc gaaactcaac
     4981 cgtacggtca actacgggga aggaagctac gggacgcagg gaagcaccag cgggtggggc
     5041 tttggagcga tgtatgaact cacctacgac gtgtacctca acgaaaaccg cagcagcgtg
     5101 ctgcagccgc tgttcaacgc ctcggtggtg acgacgcgga tggacggcta tgaggaaacg
     5161 ggtgcgggca acgcgggcct gaacgtcggc aggcaggact ggacgacggg gacgctggcg
     5221 ctgggcggcc ggtggatggg cctggtgggc agcaacatct tcggacgaga agcgctggcg
     5281 gaaatccgcg taaacgcggc gcaggacctg ggagaccgga gaggggaaac gaacgtctct
     5341 ctgctgggca accccggctt tgcgcaaagc gtgagggggg cgaaagtggg aacgacggcg
     5401 ctgcagctgg gagccggact gagcgtgccg gtgggaacga agggaaccat ctacgtgaac
     5461 gggaacgcgg acatccgtga cgggtccagc gcgctgaacg gaagcatcgg ctaccgctac
     5521 gacttctaaa agaaggaaaa cacaaggaga ggccccccac ccccgaaagg ccttcctcct
     5581 gaccctccag agccgttccg gatttattcc ggaacggctc tggatttttg tttatcggag
     5641 gatgattccc ctgacccgct tcctgaaaat attaattaat tcaaagtcaa aaagttgtgt
     5701 ttacgtttcc gcagtcaaca aaaggaaccc cgtaaacatt gaaaataccg gtatgaggaa
     5761 gaatgatgcc gctatgaacg acgtattcct tctgcaaccc tgggaatgaa tgccgctgcc
     5821 aatccgcagg atgcgctgcc ctttcagcca tcctggctgt gaagccgaaa cggcagtcat
     5881 cattcattgt ccatgaagta ggcctgcaaa atacaaatga tatgcaggac aataggaacg
     5941 agtgcaaaga tgaaccaata caggaggccg taagccgcac ccaccaccag gaaccagatg
     6001 atggcctgga caaagtggcc cttgtacaac tggccgagac cgggaataaa taggcttaaa
     6061 atggctgcta aaaaagattt catatgctgg ctgggtaaga aatgaccgca cgggaaaagg
     6121 ttcacgtgcc tgtaccggtt aagagccttc cttcttccct cacgttcaat ctcccagcgc
     6181 agcccctgtc atgaatgacg gttttcccgg agcgaatgaa gccgacgcgg cagggaacgg
     6241 gaggcagtca tgttcaagtc ttgaccgccg catgggctat gttaccctcc ccgcgcacag
     6301 ggcactcatg ttagaagggt agagaccacc atgtccattg aactgactcg caatttctcc
     6361 attatcgctc acatcgacca cggaaagacc accctttccg accggctgct ggaaaagacg
     6421 aataccattt ccgaacggga gaagcaggat cagcttctgg atgccatgga cctggagcgg
     6481 gaaaagggca tcaccatcaa gtcccacccc gttaccattt tctacaaggc cggggacgga
     6541 aagacttaca agctcaatct gctggatacg ccgggccatg tggatttctc ctatgaagtg
     6601 tcccgttccc tggcggcatg cgaaggagcc ctccttattg tggacgcagc tcagggagtg
     6661 gaagcccaga cacttgccaa catgcacctg gccatggatc tgaatttggc cattatcccc
     6721 gtcatcaata agattgacct tcccagcgcc aacctgccca aagtgtaccg ccagctggag
     6781 gacatcgtct gcatccccca tgaggaagcc atccatgcct ccgccaagat gggcatcggc
     6841 attgacgata ttctggaagc cgttgttcag cgcatccctc cgcctgaaac ggaacaggac
     6901 ggccttctgc gcgccctggt gttcgattcc gtttacgacg cctaccgcgg cgtagtctcc
     6961 tatgtgcgcg tcatttccgg cagcgtgcac cgcggcatga aagtcaaact tttcgcaacg
     7021 gacgaagtgt atgaagtgaa ggaagtgggt atcttcactc ccaaaatgac ccgaacggat
     7081 tccctggaag ccggtgacgt aggctacatc attgccaaca tgaaatccgc ggctgatgtc
     7141 aagatcgggg acacgtatac ggattacatg cgcccctgcc catcccccct gcccggtttc
     7201 aaggaaatcc gccccatggt cttttccggc atttatccgg tggattcctc cgattttgaa
     7261 gccctgaaag cggccatggc gaagcttcag attaatgacg ccgctttctc cttccaggcg
     7321 gaatcttccg tagctctggg cttcgggttc cgttgcggct tcctcggcct gctccatatg
     7381 gaaatcatcc aggagcgtct gcgccgggaa ttcaacatgg atatcatctc cacctacccc
     7441 tccgtgattt atgaggtcac caagaccaat ggagaggaaa tcaatgtgga caatcccagc
     7501 ctgctcccgg aaccgcagga aattcaggaa atccgggaac ctatcgtcaa ggtgttcatt
     7561 atgcttcctg gggaatacat cggggatatc atgcaacttg tcctggaaaa acgcggcagc
     7621 gtggacaata cggagaccat tgacgacatg cgcgtaatgc tcacctgcac cgtccctctg
     7681 gcggaaattc tggtggattt caacgacaag ctcaaatcca tgacgcgcgg ctacggctcc
     7741 atggattatg aatacgccgg ctaccaggcc gccaagctca ttaaaatgga catgctcatc
     7801 gccggggaac cggtggatgc tttctccatg atcgttcacc aggacaaggc agcctcccgc
     7861 ggcagggaac tggcggaacg cctgaaaaac gtcattcccc gccagctctt caccgtcgcc
     7921 atccaggcct gtattggcgg aaagattatt gcccgggaaa gcatctcccc catgcgcaag
     7981 gacgtgacgg ccaaatgcta cggcggggac gtcacccgca agcgcaaact tctggaaaag
     8041 cagaaggagg gcaaaaagcg catgaaggcc atcggaaaaa tcaacattcc gcaggaagcg
     8101 ttcatcaagg tgctgaaaac gggggattaa cccattttct ttcctcaagg catccaaacg
     8161 gggtcttccg tttccttccc ggatttatac atttttccgt ttgaatatgg gccatgaaac
     8221 acctcctgca ccacttcccc aaccatcccc gggaggagga atgctacatc aggctggccg
     8281 gctaccggga agaggaggaa atcgacgagc tgatctaccg gaccgtgggt cgcgttccgg
     8341 ggtccgaatt cctgttcagg ggaaatcggg ggcaaacggt acgaaaagaa gctgcgcacc
     8401 ggcagggtga gccacatcaa cgactgctac acgcatcccc tggatatcgc catacgggac
     8461 cggaagcaga aggaacagga agccaagaaa aactgaaacc gccgcggccg ttgaacaatt
     8521 cccggttgcg catttttccc tgttatgcta tcaggtcagc catgagtctg cccaggctgt
     8581 ccatgctcct gtttgcgtcc gcggctctct gttcctgcgt gggaacctct ccggaacagg
     8641 aagaaccgaa ggtaaaaaaa agcctttcgg aatacatgca ggataacaag accaattcca
     8701 tcgccaatgt gccgacgggg ctgttgagca ctactccgaa tcagcaacag gccacccatc
     8761 ttaccgccac cacacaggag gaaatgacca gggcagacag cggggccgtc tattacacgg
     8821 acgctcatga cccggacgcc cccatccccg ggctggaaga agctttcgcc cagcgtaagg
     8881 aaaacgaacg ctggatccaa agctatccca ccgccctccg ggaagcccag agtaccggaa
     8941 aacccattct catctggttc caccattccg tgggcagccc tcccagcaaa aagctggggg
     9001 cagaactcct ccatacgaag gaatttgaag actgggcgaa aaggaatgtc gtccgcgtct
     9061 gttacgacca ggcggaaaaa tttgaaagcg aacctgttta caggaagcgc cagaagatgc
     9121 tggagtatgt gaaaaaggct ccctccctgt ttggcgtgag aggaacgccc gtgcttctgg
     9181 tcatgtctcc ggacggctcc aaagtggata cgttgcgcgg ttattatacc ggacaaaacg
     9241 ccctttattt tgaccagatc aagaacagcg tcaaactggc gaagcagcaa tatgaagagt
     9301 ttaaaaaaac gctgattccc aaaggctacc gggtctggac aggagtcaac ggaaataccg
     9361 tcttcgccaa actttcccgt tattcggaaa agacccagac cctctggctt caggagctgg
     9421 acgggcacca gagcagaaca tccctgaaaa ggctcagcct tgaagacaga acatggctcc
     9481 tggagcagaa agaatcccat gaaaacaacg gccggaacaa gcgctccggc ccacgtggcg
     9541 cctgaacggc ttttctcctg ccgtccggct gcggaacggc ttattcatgg gcttccgctc
     9601 catccccatt tttgtattga aatatgaaac tgatgtttta tttttcctaa aactctcttt
     9661 cagcgttttt tctttcgggg atgtggtctt ttccgtaccg gcaggaatgc ccttgcggac
     9721 aaattctgcc gcatccccct ccaaccattc gcctgtttaa ttcatgattt caacaatgaa
     9781 ccgtcatgcc gccaccgccc tgatgcttgc ggcctgttcc ctgtccgctt ccgcggacca
     9841 gcctcaaaag cagacgccgg accagcgccc caacatcgtg gtcattgtca ccgacgacca
     9901 ttcctaccag accctgggca cctgtgaaaa ggattctccc atgccttatc cgaacttccg
     9961 caaactggcg gacgaaggca tggtctttga ccggagctac tgcgccaact ccctgtgcgg
    10021 accttcgcgg gcctgtattt acactggccg ccattcccac atgaacgggt acctcttcaa
    10081 cgaacatgcg gctccctttg acggttccca gcccactttc ccgaaaatgc tccaaaaggc
    10141 cggttaccag acggccattg tcggcaagtg gcacctggaa gccattccgc cgggcgccaa
    10201 gggagatacg tccaaatatg aatccgaccc caccggattc gattactggg aaattttccc
    10261 cggccagggc aactatttca atccggattt catcactccc ggcaaggacg gcaaacgcgt
    10321 ggtgaaaacg gagcccggct atgccacgga actggttacg caaaaaagcc tcaaatggct
    10381 ggaccagagg gacaagaaca aacccttcat gctcgtcgtg ggccacaagg caccccaccg
    10441 ttgctggtgc ccctccattc agaatctggg ccgcgccaaa cagtatgcgg acgccattga
    10501 cccgcccgcc aatctggaag acgattttgc agaccgcccg gaattcctga aaatgacgga
    10561 acaaaccctg ctcaaccatt tcaacgtatg gtctgacgaa cacctgatca aggaggtggt
    10621 ccccgaagac atccagaaaa tgctttcctg cccggaatcc aagaccctgc atactcagta
    10681 tgactgggaa atgccggaat gggtgcgcat ggacccgcag cagaaggaag cctggtacaa
    10741 ctaccacaag gcccgtaccg tacagcttgt caaagatatt aaaaacggga aaatcaaaac
    10801 gcagcgcgac attttgctgc gccgctggcg ccattatatg gaagactatc tgggcaccgt
    10861 tctttccgtg gacgaaagca tcggccagat catggactat ctgaaacaaa acggtctgga
    10921 caggaatacg ctggtgctct actgcggaga ccagggattc tacatgggcg aacacggcct
    10981 gtacgacaaa cgctggattt tcgaagaatc cttccgcatg cccctcatca tgagatggcc
    11041 gggccacatc aggccgggcg tgcgctcctc cgccatggtg caggaactgg attatgctcc
    11101 cactttctgc gacgtggccg gggtaaatac caaggaaaat atgaatacct tccagggccg
    11161 cagcctcact cccctgttca agaccggaga acatcaggat ttcaaaaacc gttctcttta
    11221 ctacgccttt tacgaaaatc cgggtgaaca caacgctccg cgccatgatg gcctgcgcac
    11281 ggaccgctac acgctgtcct atatctggac cagcgacgaa tggatgctct ttgacaacca
    11341 gaaggacccg gcccaaatgc acaacgtcat caacaaaccg gaatatgcgg aaaccgtgaa
    11401 agagctcaaa gccctgtacg gcaagctccg caaagactac caggtaccgg aaggcttccc
    11461 cggggccacc ggcaaactgg ccgtcaagcc gcagtgggac tgcgctccct ccagagattg
    11521 acgccgccat tcaccgttcc tcccgaaaca ggttgtccgg aacacgccat tgacgacgga
    11581 gggacagggt ttaaatcctc acacaaacaa caaccattac catacgcgcc gcatgaaatc
    11641 aattttgcta gccgcagcgt tcctgggctc cctctgctgg gccggaacca atccctacaa
    11701 cattattccg gagcccgtca acgtgacgac aactcccgga actaccaaaa acctcaaaat
    11761 cgtccatgag caaaaagtcg ccggactggg caatgaaggg tatgccatga aactgacgcc
    11821 cggcggcgtg gaactccgtt ataccacgcc caacgggaag gccatggcca tggcgaccct
    11881 gttccagctc caggaccagc tttcggatac tcctgaggga cttccctgcg gcagcatcca
    11941 ggattccccc gacttcggct ggcgcggcat gatggttgac gtaggccgct accactatcc
    12001 catgaaggag atctacaatt ttgtggacgc catgcattat tacaaataca acgtcctgca
    12061 tctccatctg acggaagacc agggctggcg tctccccgtg ccgggctacg acaagctccg
    12121 caccatcggc gccgtccgcc cctccgctcc ggaaagccag aacaactccc tgctggccaa
    12181 tgaaggcatg tataccaaga aggagctcca ggacctggta gcctactgca aagcgcgcgg
    12241 tatccaggta ctgccggaag tggaaatgcc gggtcataac atggccctgg ccgcgtccta
    12301 tcccgaattc tgctgcaaca ccaaacgggc ccaggtatgg acgcacggcg gtgtttcctc
    12361 caagctgatt tgcccgcaaa aaccggccac taaaaagttt cttaaggata ccttcaatac
    12421 cgtccagcag atattccctt tcccgtacat ccacatcggc ggtgacgaat gccccatggg
    12481 ggactggaag aagtgcccgg actgccaggc cgcccgagcc aaaaagggcc agggggataa
    12541 tgtggaagcc cagatgagcg atttcacgaa aagcctgacg gccatgctcg ccaagcaccg
    12601 gaaaaagccc atcctgtggt atgacatcaa caagagctat taccacaagg gggaaaccgt
    12661 catgtcctgg ctgccgggag aattcccgcg ttgcattgat aagacgaagg aacagggcat
    12721 cgacctcatc gtcacccccc agttcaagta ttatctggcg cgtacccaga tgaaattccc
    12781 ggcggacgac gtgcgcgccc ggcccggcgg agctcccatc ctgctgaaag actgctacaa
    12841 cttcgatccc cgcaacggac gggacaagaa tgacgtcaag cacatcaagg gaatcaacct
    12901 ctgcatgtgg gcggaatgga ttccctccgg cgaattgctg atgtacatga cctacccccg
    12961 cgccatggct gtttccgaaa ccgcgtgggg cagccacaag aaccgtccaa gcctggaaga
    13021 gtttgaaaag aaaatggaaa cccacaagaa acatttccag aagcgtttcg gctatactct
    13081 ggaacgcact gtggaaaaca aaccctaccg ggaaaaattc atcacccagg aggaaatcga
    13141 acgtattaac gagaattata aaaagggcca gcaaaacgcg gacaaatagc gtttcccttt
    13201 ctctcgccat cctatgattt cttcaaagcc ggggacgcgg acaaccgcgg atccccggct
    13261 ttcttttccc gcttgaccgg gaagccctct tcccttctaa tcgttccgcc atgttacacc
    13321 tttacgatac ccgcaccaga acggcccagg acatttctcc catggatgga aaaacactgc
    13381 gcttttactg ttgcggcccc acggtgtacg gccctgccca catcggcaat ttccgcactt
    13441 tcgtgatgca ggacgtcttc cgccgcgtcc tggaactggg gggggtgccc accacgcata
    13501 tccgcaatct gacggatgtg gacgacaaaa ctatccggga ttctcaaaag gctggcgttt
    13561 ctctggcgga attcaccgca ggctgggcgg atctattcca ccgggactgt gccgctctta
    13621 attgcctgcc tccccatgcg gaaccctccg ccgtgggcca tattcccgaa caaatacgga
    13681 tggttcaaac actggtggaa aagggccatg cctatgtatc ggaagacggt tccgtgtatt
    13741 tcagaatttc ttccttcccg gaatacggaa ggctttccca cctggacgaa cgtgaactgg
    13801 atttaggaaa aaccgccaat acccggtcca acgcagacga atatgaaaaa gactccgtgg
    13861 cagacttcgt gctgtggaag agccgcaggc cggaagacgg aaacaacttc tggccctctc
    13921 cctggggaaa aggccgcccc ggctggcacc tggaatgctc cgccatgatc cataaatact
    13981 tcggcaatga ctttgatctc cactccggcg gcgtggatct ggtattcccc caccatgaaa
    14041 acgaagtggc ccagtcccgc tgcgcctgcg gcggaggctt cgcgcgcctg tggttccaca
    14101 tcacgcacct gctggtggac ggaggcaaga tgtccaaatc cctgggcaac atgtacacgc
    14161 tggcggattt ggacaaactg ggccacaggc cgtccgcggt ccggtacgtg ctggcggggg
    14221 gctattaccg ccgtccgttg aatttcaccc tttcctctct ggaagacgct aaagccgcgc
    14281 tgaaccgcct gtccaaattc gatatgcagc tcaggaacgc ctccggaacg gattccgttc
    14341 cctcctatga ggaattctgc gcggcattcc cggaattggg gattttccag ccggcatggg
    14401 acagcctgaa cgatgacctg aacactccgg aagccctggg ccatgttttc agtgccatca
    14461 ggaaggcgga tatcccctcc ctttcaccgg aggaggcggc ccgcctgcgg aatgccttcc
    14521 actttattct ggccgccttc ggcattattc tgccggagga gggacaggag gaagccccgg
    14581 aagaaatccg caccctggcg gatcagcgct ggcaggccaa gcagaaccgg gactggacgg
    14641 aagccgaccg cctgagggcg gaagtggcag cgctgggctg ggtcattaaa gaccgcaagg
    14701 acggatacga cctggcacgc aaataaacaa atggacgcct tttcctctat tcccaatcat
    14761 acgggcttca cggccaggaa cctgttcatg gacagtcagg gaatcctgag aggcggagcc
    14821 ttcgccaaag tagcccccgg cggcggcggc cccctttccc cgcaccgcca tgcccacgct
    14881 catttattca ttgtcacccg tggaacgatt tctgtcatgc ttgacggaga agaaagaacg
    14941 gttcacgagt acgaatccca gctcgttccc ggcggcgttc tccatgccgt ctggaacagg
    15001 aatacggaac cggcggaaat cctgggactg acgctggaaa cgccttcctc ccccacatcg
    15061 tcaatttaat gagttcccga cagcgcaccc tgggccatgc cgccgccctc atgaccatta
    15121 tcatctgggg aaccactttc gtctccacca aggtgctctt gcgggatttc acccccgtca
    15181 cggttctctt tacccgcttt gtgataggat atgccttcct ctggtgcctg aaaccgcggt
    15241 ttcttccatt ctccggctgg aaaaaggagc ttctgttcgc cggagcggga ttgaccgggg
    15301 ttaccctgta cttcctgctg gaaaacatcg ccctgaccta taccttcgcc tccaatgtgg
    15361 gcattatcgt agccgtggtt cctttcttca cagcccttct ggcccatttc ctgctgaagg
    15421 gggaaggctt ttcacgccgt ttcttcctgg gtttttccgc tgcctttaca ggtatcttcc
    15481 ttatcatggc aaacggagcc ttcgttctgg aactgaatcc ggcgggggat atcctggcac
    15541 tgggcgcggc cttcgtctgg gcggcgtatt ccatcctgat gaaaaaaatc ggcgtcaaca
    15601 catccaacat gatcatctgc acgcgccgca ttttcttcta tggcatcgca ctgatgattc
    15661 ccgccctgtg ggtccttcct gcaaacatgg actggcgcct gatggcaaaa cccgtcaatg
    15721 ccatcaatct gctgtatctg ggtctgttcg cctccgcact gtgctttctg acctggaacc
    15781 gcgtggtgga aattctggga gccgtcaaat ccagcgttta catttacatg ataccggtcg
    15841 taaccgtggt ggcctcagcc atcattctgg gggaacgcct gacctggata tccctggcgg
    15901 ggattctgct taccctgtgc ggcgtcacga tttcagagta cagaaaaaaa acacggaaaa
    15961 gcgggaagaa aaggctatcc tgaaagaaag ccggtttctc ccgggccggt tccctctatt
    16021 tttccccttg catggaaatg ggcggaaacc cttccaggcc atgtcaggct gatgattttc
    16081 cgcagcggac aaatattcat ttattactcc gcaggaaaga ttgcctccgg attagatatc
    16141 cggtgacaac aaaatttttt gaagaaaaac gagtttcacc acgggaaaag aggaaaaatt
    16201 tggaaagtgt ttaatacaca ataaaatttt attttcataa atcattagta atgtgtgtat
    16261 ttatccattc ccattttcct atcctctgtc accggatatc taatccgcta acatggagct
    16321 atgaagagtc ttcttttcat ttcaacattt ttattgtgta tttccggagt acaggcggcg
    16381 tccactctgt atgaaaccca gtttcatcaa accagcatta ctactgggag cgattttctg
    16441 gaagatgctt ccgtcactct caccactcct tccaccgccc tctccaacaa tctggcagcg
    16501 gatcttcttg ttcccaacct ccaaatgaat ggaaatgaaa ccggttggac tgtcactttt
    16561 tccttcacag ctacgcagga catcgtcctt tcttccctca gcctgggttt tcaattcgtc
    16621 aatgcaagcg gggagcgtca tagcaatacg gatactaaag cgggaaccgc taccgttacg
    16681 ctgactgccg gagattcctc cgccacggct gacctgagct ttgagcgagt ccaggctgat
    16741 aaagacggca cgccgacggc cgatatccag gaagccagct tcaatacccc ggtcacagtt
    16801 agtgcggggg aaacctttac cctcaccgtg aatgccaagg ctcccaacac cggaggaacc
    16861 ttcctggggc tttcccagtt gaatcttcag ggggacacgg taccggagcc ggcgacggcc
    16921 tccctcagcc ttcttggcat ggccgccctg cttctgcgcc gccgggtata accggaaaag
    16981 cgttgaagtt ctgatttttt cctctgtccg aacagggctg ttcccggaaa cagccctgtt
    17041 ttttgttgag aaatcctctc agcttccgga aacgtttctt tccgcctctt cttgaatgcg
    17101 ggcttccgtc tcttcaatct tttccgaaag atcagcccat tgtgtaaaac aggtttccag
    17161 ctgccggtcc gtctgctgaa aacgggaggt aagctccatg acggcttccg tatcagccgc
    17221 cacttccgga cgctccaatt gagaagtaat ttccgtcttt tccgtttcca gccgcgctat
    17281 ttcttcttcc agcagctcca gtttctcctg aagcggacgc agcaggcgcg tcttcttctg
    17341 acggatttcc gcctccatgc gcctgcgtgc cttgcgatcc aatccggcgc ccggttccgc
    17401 cgctccggag gggggtgagg cggctgcggc aaccagggcc tggtccctct ccaccttttc
    17461 caaataatca gaaacattgc ccaggtaaac gcgcggtttc tctcccggta caaattccag
    17521 cactttcgtc acgatgggat ccaaaaaact gcggttgtgg gagacgatgc agtaggaacc
    17581 cggatattcg gacagagcct tctgaagcac ctgctgggac tggatgtcca gatgattggt
    17641 cggttcatcc agaatcagga aattggccgg atgcaaaagc atgcacacca gcgctacgcg
    17701 ggagcgctcg cctccggaaa gcacgccaac gcgcttgtgt acgtcatccc cccggaacag
    17761 gaaacagcca agcagattcc ggaccatggg cgcagattcc cgcgtggcgg cagcttccac
    17821 gcactccaga atcgttttat ccggatccag atcatcagca tgcgtctggg agaaaaaagc
    17881 tacctgcgta tggcgtccca tggtcacgct gccggaactg ggggcctctc cgccggaaat
    17941 aaggcgggaa aatgtggatt tccccgcccc gtttactcct acaatggcaa tgcggtcccc
    18001 cttgacgatt tcaaaatcca cattctcaaa aacggaaacg gaaccataat gtttggaaac
    18061 cttttccagc ctgaccacag aatgggcgcc agcgggagga gcaggaaagt gaaagttcat
    18121 gacggcatca tcctcttcca cctcaatcag ttccactttt tccagctgct tgatgcggga
    18181 ctgcaccaga gaagccttgg tggctttata acggaaacgg tcgataaatt ccttcgtttt
    18241 ggcaatctcc cggtcctgag cctttttctg gcgcagcaga atttccttcc gcagcacgct
    18301 ttctttcaga aaataggaaa aattgcccgc gtattcctcc gcccggccgt ggtaaaaagc
    18361 aatcgtacgg gaaaccagag catccagaag cgccacatca tgggaaataa ggatgatagc
    18421 gccccggtag gagcgcagat attgttccat ccaccgttgg gactggatgt ccaaatggtt
    18481 ggtaggttcg tccagaagca gcacttcagg ctcccggagc agcagtttgg ctaatgcgat
    18541 gcgcatttgc cagccgccgg aaaattctcc gcaatcccgg tcaaaatccc tgtctttgaa
    18601 tcccaggccg cgcaggatgg attccgtccg gggacgcagc cgggcggaat tatgggcatg
    18661 cagcaccagt tccaattccc cgatttcatt gagaacttca gagtaggcgg cggaacgggg
    18721 atccagcccc gccatttccc gcgtcaggac atcaatgcgc tcctgaagat ccatcaggtc
    18781 cgcaaaggcg gacatggctt catcaatcag cctgcgccct cggatatgaa ttccttcctg
    18841 gggcagataa cccacctggg aatgcctgga tttgataatg gagccggaat ccggttcatt
    18901 caaaccggcg atgcacttca tcagcgtgga tttccctgcg ccgttatggc ccgcgagggc
    18961 aattctttcc ccatcctcca ctgtaaagga aaggtcattg aacaggacgc gcgcgccata
    19021 ctgcacacgc aggttttgaa cagacagaac catgtactga aaaagcagag agaggaaaac
    19081 agcggaacgg gcgcccccgc gccgcgctcc gccgagtgaa tgccggaatg aggttactta
    19141 ccaagcgcct tgttccacgc ggcaatgcgg tcggcctgtt ccgtaaacag atcgtccgca
    19201 ttatcttcaa tcgtaatgcc ggagatggta tctccctgct caatggaatc caccacgctc
    19261 tgaccttccg tcaccttgcc gaagacggtg tgtttgccat tcagccagtc ggtgggcaca
    19321 tgggtgatga agaattggga accgttggtg ccggggcccg cgtttgccat ggaaagcacg
    19381 ccggggccgt catgcctgag gtccgggcgg cactcgtcct caaacctgta gccgggcccc
    19441 cccatgcccg tgccggtggg atcgccgccc tgaatcatga aatccgggat cacacggtga
    19501 aatttgagcc cgtcataaaa tcccctggac gccagattca gaaaggaagc cgccgtcacg
    19561 ggcgtcttgg aagcaaacac cgtcagcttg atgtcgccct tggaagtatg gatggtgata
    19621 tttttgtcag ttgccatggg tatatgttaa acgcgcggaa aggcatggta aaggcttatt
    19681 gcgtgcagtc cccgggcccc tccgtcattt ccctccagcc actcttccgc ctctttccat
    19741 gccgggaaaa agaccgccgc cgcgcctacc tgtcatgata gactctaatg acaggaggaa
    19801 cggttgattt tccacaaagg aagaccgaac ttgaatggca tgagcgaaga actggtagaa
    19861 gaattcatta cagttgaatc catctttgtc cgcggacgca attgtctggc gctcaggtca
    19921 aaattttctc cactgtttac ggattactac cttcacctca tgcagtacgg cctgcgcaac
    19981 gaagaactct acgacagcct gctgaaagaa ctcatggcct atttcaccct ttacatggtc
    20041 gcacggccct gggcggaaca acatgcctgg acagtcaatc tccggacgcc tgccgtcgcc
    20101 aacctgtttg tcaccggttc ttccctgacg gaatccgtcg tggggcgcgt cttcacacag
    20161 gacattaagg aacctgaaga aaacacgctg tattccaccc tgctccgcaa ggggtttgaa
    20221 ccgcaatact ccgtcgtacc cattccggga acgtctccgg cccggtgggt ggaggaattc
    20281 tacaggcaaa gcgaacagag aaacgcccgc tgcattgagc tcccggatga atgctacacc
    20341 atggtgacgg cccagcctga cgcggatgaa gaatggcttg cttccctcac ccgggagaaa
    20401 atggcccatc tggaagaaga tgaaaatacc cgagtgctgg aaacacgccg gttccgcttt
    20461 tactgcggct gcacgctgga ccgcatcctg cccacgctca agacccttca ggacaacaag
    20521 gaagacctgt tccagggaga agaggagctg gaagtcacgt gccccagatg cgccgccatt
    20581 taccgtgtca cgaaagaaaa tctggaagat tgagacatac atgatttctt tctgacaatt
    20641 agtcttttcg gcaacgaact tcgtgcttaa actttccttg tgccgacttg tccgaagtgt
    20701 ttacataatt tccaccgcgg ggcggaatca ttgtgccctc actgcggttt ttccctggaa
    20761 aacctggaca aaaaatacgg caaagacgcc ataccctacc gccgcgtatg cgacaacgcc
    20821 ggcgctctcc ggcagcagga ccgcatgagg ctgaatgccc tgctggagaa actggaacgc
    20881 cgcatccccc ctgttctcct gtccgtctat ttccccaaca tcctggagcc cttttccctt
    20941 attccgcaca atttctggac gatgaaccac ctgacggtgg atgaggccgg cttccccaac
    21001 catcagggac cgctggatcc ccagtggctc ctggttctgg tgctggacgt gcgcacggat
    21061 acggcatgct tcatgtgggg ctatgaactg gacccttatg tagagccgga cctgatcaac
    21121 aaatccatca tgaaagcccg cattcccctg cgggaaagca tgctgctcca ggctgcgggg
    21181 tccattatga aaaacgccgt ccagctcgta gccaggaaag cgcgctccaa agtcaggcat
    21241 cccatgcgct acggattaac gcctcccacc cccgaggacc ggaaaggagg aggagcatga
    21301 accccttttt gttcccatgc ctgctggcgg cggccatctt tccggctccc gcccaggaat
    21361 tacccgccct gaaatggacc aaccgcgacc agcaatccct gatgagaggc gcgtggtgga
    21421 atgccaaccc tcccttgctg ccccctgtgg atgcggcagc ggctccctcc gaccccatgc
    21481 cggaacccgc cgccaatgca gactcccccg ccgtggatcc cattccggag gactttacgg
    21541 acgtcaatga atatttcctg ccggactaca tccgcacctc cacaggcctg attgaccccc
    21601 agaagcttct tgcggaagtg gaaagcaatg acgtcatcga acttatcaag ctcatcaaat
    21661 cccgctacaa cgtcaacctg ttcgtcagca tcttcgcggc cgggcagaaa gtcccccctt
    21721 ccgtcaatgc tcccaccatc gcccgccaga tattcaggaa caaggagcgc aacctgctgc
    21781 tccatttcca catgggggat attaaaagcg cccagatcgc actggatccg gagctgagct
    21841 cccagcttgg ggatgcggga cggagggatc tcctgtacca ggtgaagcag gacgcctccc
    21901 gattcaccaa tccgcaggac gaactcattg aggccatgat ttccctggtc agaa
//
LOCUS       DAWEMX010000030        21426 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1029_length_21426_cov_11.405082, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000030 DAWEMX010000000
VERSION     DAWEMX010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 21426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21426
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_21426_cov_11.405082"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12578..12587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13838..13847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16062..16071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17480..17489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18133..18142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4998 a         6490 c         5563 g         4325 t
ORIGIN      
        1 tccaacgtgg tttacatggc tctgttcggc gaccataaga atgagggtgt tccttccagg
       61 gatacaacca tttacaatga tgagttgaac ccctctacac agcccaaaag caacccgact
      121 gtccgggaag tccatgtgaa aagcaaggac ggcaggccgg ttacccttta tattcttgcg
      181 gatccatggg gttcccccta ccgctaccgc ctgggcagcg agcagtccat ccccaccaga
      241 accgacaggg ccagaaacct gaaaatgggc aacggcctga atccggatta tgatttctgg
      301 tctttcggaa aagacgggga cagtgatttg aaagacccgc acgcccctga aaacgaagat
      361 gacatcggca acctgccgaa gttctgacct taccggtatt tttccgcgca cggcttcttt
      421 tccggcagcc ttcctcccct tccggcggag gaaggctttg tgcatatttt ctggcatccg
      481 cacggcttct gaagtatgat tcgggcactg acatgaaacg tatttccctg atcgttccct
      541 gcctgaatga agaagaagcc attccggcgt tcaagcaaga aatagaccgg gttttcaagg
      601 aatgcctgga gggctacgcc ccggaactta tcttcgtgga cgacggttcc acggaccgct
      661 ccctggaaat gattaagaaa atggcggaac actcccctga agtaaaattc atttccttct
      721 cccgcaattt cgggaaggaa gccgccatct atgcgggcct ggaagcggcc acaggggatt
      781 atgccgccgt catggatgtg gatcttcagg atcctccttc cctgcttccg gatatgctga
      841 aagccattga aaaggaagga tatgactgtg cgggaacgcg ccgcgtcacc cgtcaggggg
      901 aaccgctgct gcggtccttt ttcgcccgcg ccttttataa gacgattcat cagatatcgg
      961 acacccgcat cgtggacgga gcccgggact acaagctgat gactcggccc gtactggaag
     1021 ccctgctttc cctgaaggag tacaaccgat tttccaaagg gctgtacgaa tgggtggggt
     1081 tccggaccaa atggtttgaa tatgaaaatg tggaacgggt ggcaggagaa accaagtggt
     1141 ctttcttcaa gctgttcctg tactccattg aaggtatcgt ggccttttcc accgcccccc
     1201 tggcgctggc ctccatcatg ggggtacttc tttccttcat ctcattcctt tccatcatta
     1261 tcttgacggt ccgcgaattg atctggcccc attccgctta cggatggact tccctggtat
     1321 gcgtcttctg catgatgggg ggcatcgtgc tgctgtgcct gggcattttg ggccaatatc
     1381 tggccaaaac ctatacggaa gttaaaaaac gccctattta catcgccagg gaacgttcca
     1441 tcaagccttt caactaacct tgcctatgga cacagaaccg gatttcagca ttattacgcc
     1501 cagctataat tacgcccgct acgtaaggga atgcatagag agcgtcaaaa accaggaagg
     1561 ggctacattt gaacacatta tccaggacgc agggtccacg gacggcacct tggatatcct
     1621 gcactcctac cctcacctga aactatatgt ggagaaggat tccggcatgt cggaaggcat
     1681 taaccgcggc ttccgcaagg cgcgcgggaa atgggttatg tggctgaata cggacgaccg
     1741 gttgctgccg gaggctctgg cggcggtcaa ggcctttgcg gattcccgcc cggatgcgga
     1801 cattgtgtat ggagcatgga atttcatcgg gccggacgga gaaatccaac gcgccatgaa
     1861 ggccctgccc tacagtctga acatgcacat ctggtacggc acctatctgg cttccaccgc
     1921 attgttcctg cgccggagca ctactattga agaggggttc ctgctggacg aacgcttcca
     1981 ctacgacatg gacggggaat attacgcgcg ccttggccgg gcggggaaga agttcgtgca
     2041 ttacaacagg ctgctggcgg attttcgctg gcatggcgac aatctgagcg cccccaatat
     2101 cgaatgccgg gacatggacg ctgagctaaa gcgccagaag cagcacggag aggatgccgc
     2161 catcaagcgc atttacggcc tttccttttc caaacacagc tgtaacaata tcatggacgg
     2221 cttcatgcgt gaggcctacc gtatgaaaaa ggccttcctt tatcttacca ctccctggga
     2281 aaagtaaccc gttctttcct gcttctccca gaaaagccgc tgccttcccc cttcatcctc
     2341 ccaaggaagg cacagccccg atacggcaag ggccgcagcc gttattacag ctgcggccct
     2401 caatgaacct gatgggaaaa aggcggcctt cccttaatta aggccgtcaa acaattcttc
     2461 ttcccggaaa aatcttgcca gctccacggc ggcggattca cggctgtcgg acgcatggca
     2521 gatgttcatc atgctgtctg ttccgaaatc gccgcggata gtgcccttct gcgccttctg
     2581 ggaattggtg ggccccagca gttcgcgcac gcgcacaata acgcccggcc ctttcagggc
     2641 aatggcgatg accggagaac tggtcatgaa atcaagcagc ttcgggaaaa gcggttcccc
     2701 attgatgacc agatccagaa tatgggcgta atgttcccgg agcaccggtt cgtcaagctg
     2761 gatcatcttt atgccgcgca ggcgaaaccc ttcggacaaa aaacgtttca aaatggtgcc
     2821 ggaaaggttt ttccgcacac agtcgggctt cagcaaaatc agcgttgttt cttcttgagt
     2881 gtttggcata aaatcagcga agtttcagga tggagtgagg gaattacacc ctaatccccg
     2941 tcaggtcaag gataattgca caactggtct tttctgttgg gaaaggaatt cgtcacaaat
     3001 tcctcttata ggaatcgcac cggttccgcc atccggcaag ccagcgcgct tctccgcgac
     3061 ccggcgggct gttgtccacg cggcgctgaa gcaccctttt agccgcttcc gcaaattcaa
     3121 ccgccgcagg ctgtccggga gaaacggggc gcatttcctc cagcacctgc ctgagccccc
     3181 agccaatccc ccggtactgt tccgccggat tggttccttc tcccttgaaa ttcacgtagt
     3241 caatcagggc atacatgccg ttgggaaccg acgccacggc ataatagcgg gccgccatct
     3301 cttccgggcg gcgggactgg cccttgatac gctccagagc ggccacagac ctggcaataa
     3361 tgaaatccgt ctgcatttgc agggccgccg ggctggacaa ccaccggcgc atccgttcag
     3421 gaagaccgcc gcgaacgtcc gccgcttcaa attcctttct ggtccgccac ggagccacac
     3481 cggaaaacca ttccggaacc cggattccct tcctgcggca gaactgaatg aaagcaggga
     3541 aactttcctc aaacctttcc gtgactccgg caggaaacca gatgaaatgt ccgattccca
     3601 gggatggaaa cgcctctccc cggttccagg aaaccagccc ccttattgtc ccggcacatt
     3661 cgttttgcca gattttccgc gcaatttttg ccttcacttc ccccgtatcc ggcgccgcct
     3721 gaacccagac gggcacgcaa cacgcaagca tcaggacaca acctgccgtt tttccatgca
     3781 tgcagatact ttccttcccc aacacagaca agtcaagccg cttcaatttc ccgtgccaaa
     3841 gccagcggtt catgctaccc ttgccgtgca cggcatgatt ctggcgttca ataaacctta
     3901 cggtattctc tcccaattta cgaaggaggc tccccaccac cggacactgg ctgaattcgg
     3961 gtttcctccc ggcgtgtatc ctgtaggaag gctggatgcg gattcggaag ggctgcttct
     4021 tttatccgat gagaagggcc tggcggaccg tctcctgaac ccctccggca ggcatcccag
     4081 aacttattgg agccaggtgg aaggcactcc ttccgaaacg gaccttcgcc ctctggaact
     4141 cgggggcctc cgcatccgcg ggcactgcac gctgccttgc cgggcacgcc tcatgcgcag
     4201 ggagcctgac gtgccgcccc gcatccctcc cgtccgctac cgcgcctcca ttcccacctc
     4261 atggctggaa ttgacgctgg tggaagggaa aaaccgccag gtgcggcgca tgaccgccgc
     4321 catcggtttt cccacactcc ggcttcttcg cgccagaatc ggaaatttct ccctggcggg
     4381 actgaaaccc ggcgcatgga aagaactgga ttcccgcgag cgaaggcagc tcatgccgta
     4441 atataagctt gattccggac aggaaccgtt tttatatctc tctccgcatc aggatatcta
     4501 cctagacctt tttacatgaa agccgaactc gtcgaacaag cctccaaaat cattcccgag
     4561 ccccaaatgc tgatcaacgt ggtttcccgc cgcgtagccc agcttaataa tggccgcgcc
     4621 ccgctggtgc ctaccacacc ccatatgggc aacgcgaaca ttgccctgac ggaaatcatt
     4681 gaaggcaagc tcgtctatca tacggaatcc ggcagcctcc aggaagacaa ccagtaacaa
     4741 cacattccct ttattgccgt tccggaccgg aattccgtac ggaatcctct ctatccagcc
     4801 aggccccggt ctcccagccc ggggcctttt catccttctc cccttcgtgc catgagctcc
     4861 gacatttcca ccaacaaaaa agcccgccgg gattacgaaa ttctggacac ctacgaatgc
     4921 ggcatggaac tcaagggaac ggaagtcaaa tccatccgcg ccggcaaggt gaatattgcc
     4981 gattccttcg ccagggtgga aaacggccag atgctcctgt acggctgcga catccagccc
     5041 tgggagacag cgggagaatt cttccagcac caggcgcgcc ggccccgccg ccttcttctc
     5101 cataaacggg aaattttcaa actggaacag cagacttccc agaaaggttg ctccctggta
     5161 gctctcaagc tgtattggaa aaacggcaag gtgaaactgg ctctaggcct gggcaagggc
     5221 aaaacccacc gcgaccagcg ctacgatttg aaagcccgcg tggaaatgcg cgaagcccag
     5281 cgggaagtgg cccgcatcaa ccgccgttaa gccaggcggc aacagcatgg ttgccgtccg
     5341 cttcctcccg gaagcctttc tccgccccag ccattccctg ataaccggca tccgtccggg
     5401 gggtatcggg accgccgccc cagtcgggag cccagaccga cttcagcgcc cgggaaaaaa
     5461 ccggctacag ccggccgggc atgcatgcag gaaaagttac aatttccaat ccacgggcaa
     5521 ttccacctgg ccgccctttt tatcatcata acggatgaaa ccgtgttccg ccccgtacca
     5581 gtgcacatcc cgcgtcacct tcacgtcaaa acagcaataa cgggctatat ccattaaaag
     5641 ctccgtatta cccgtctgcg cgtattccgc ccaccatttg agagcctggg tgcccacggc
     5701 cgtttttgaa gaaccgatgg aggcggccgc cacggaatcc agcttgacgc gcactccccc
     5761 gcgctgttcg atgtccttgc acaaatcaag gttgttcgtg atatcaatca tgttccacat
     5821 cgtataacgc tggagcacgc catagtcgaa atgctcgtgg ttgtaaccca ccaccaggtc
     5881 ggcctgggaa agctgccgga tgagatcatc caccatttcc tcagaataaa tcgtatattt
     5941 gccggaagcc gtggaataag tcacgcccac ggaaaggcgc atctcggccg tattatgcca
     6001 gccgcccacc tccgccgcgg aatgacgggt ttccaaatca aaatatacga tgtctctcat
     6061 cgggtgaaaa cgtacattat ttgccacttt tggacaagtc ccacttgaac gggaggcctg
     6121 tttgtgatga gaataagcgc atgcagcccc gtttccacat cgtcatgttc catccggaaa
     6181 ttccgcataa tacaggagct gccggacggt tggcgctggc aacgggttcc aggctccatt
     6241 tgatcaaacc tttggggttc agtctggatg aaaagcatgt gcgccgtacg ggtctggact
     6301 attgggccaa ggtggacctg catgtctggg acagcctgga ggaattgaag caggcggcag
     6361 cccccggcgc gcagttctgg tacctgagca ccaaggcgcc acgttcccat tgggatgcgg
     6421 ttttccacgc aggggactat ctggtttttg gaccggaatc ccgcggattg ccggaaagca
     6481 tgctgagaga acacgcagat accgccctga ctattccgat gcccggagaa ggagcccgca
     6541 gcctgaatct atccacagcc gtagctgtcg tcctttatga aggactgcgc cagaccggca
     6601 tttgacgagc cccccgcctg cctctccaca tcggcatgga tgttctgcgg gttccaccac
     6661 caaagaacac agggccatga agaggcgggt tccgcgtctt tcctccgtca gggacatacc
     6721 agataagata gaatcaagga agagacggct ccgcagatat cccagaacat acccttatgg
     6781 gaatcccgta tagtctccca gggaactgaa aaaaatcatc tgcttcatcc ccacccgcga
     6841 taacggcata ttgctattca ataatttctt gagcttccgc tacggctata atggaaaaag
     6901 cccaggacaa tgaacagtac ggttgaatac cgccggcgga tagaaaggat attttcacga
     6961 aaaaaagcgg aaggcagatg caggaaaggc gccggatatt gcttccggcg cctttcctga
     7021 ttaaaacctg tcaccgcatg tcgatcgtag gaaccggaat gcggtccttg ggccctgtgt
     7081 aaagagtcag cggcctgtac agcgtgttat cctccatttg ttccagaacc tgggccaccc
     7141 agccgctaca gcgggcaatg gagaaaatgg cggtaaacag cctggtgggg atcccgatgc
     7201 tgtaataaac cgtggcggaa taaaaatcca cattggcatt caagcccttg cgctcccgga
     7261 caagtttcgc gatgcgttcc gacatgcgga tccacttggg ttccccgatt cgggaggaaa
     7321 ggcggatggc catgcgtcgc aaatgcgggg cgcggggatc cagcacgcgg tacacgcggt
     7381 ggcccatgcc catgattttt tccttccgcg ccagcttgtc ctcaatgtag gagtccaccc
     7441 ggtcctccgt gcctatttcc ttgagcattt caatcacgcc ctcattggcg cccccatgaa
     7501 gcggcccctt cagcgtgccg atggccgcgg tgatggcgga atacatgtcc gaaagcgtgg
     7561 cgatgcagac gcgcgccgca aaggtggagg cattcatgcc gtggtctgcg tgcagaatgt
     7621 aggctacctc cagaatatgg gcttcatact gatccggttc ctcacccttc agcaaccaca
     7681 ggaaatgttc cgcctcgctc aggtcctccc gaacgggggg aagctccaga ccctggcaca
     7741 aacggtagta atacgccacc atcacgggaa ccttggctat gaggctgagg cctatttcct
     7801 tcaactgccc ggcgtccttc gtccggtcgt catacatgcc gagcatggaa acagccgtcc
     7861 gaagggcaga catgggccgg gcggacttgg tggccgtttt gaagaaatcc agaatgggct
     7921 ggggcaggtc gcggtcggaa cggaggcgtt tcttgatgcc ctccaattcc tcacggttgg
     7981 gaagacgctt gttgagaagc agataaacca cttcttcaaa acagcacatc tccaccagat
     8041 catcgatatc gtagcccaga tacaacagac gcccttcctc gccgcgcacg tcactcaaag
     8101 cggattcatt ggcgatgatt ccttttaaac ctttgggata tgcgaggcgc tcggaaaaag
     8161 tgtcattgct catgttaaaa aatgctactg gtggtgaata ttaccggtta aaagtctaac
     8221 aatcttccaa aaagtcaaac cttaattacg ccttcacggc ccatgcggga cgtttggcgc
     8281 gggtgatgtg cggagccctc acatacagcg gttccgccgg actttccaga agttcttctc
     8341 tgcgggcctc cgccagggac agccacgcgg ccccgagcgc ctctgcagaa ggatacacgg
     8401 ggacaatccc cgacatttcc cgggcggcca gatgttccgc cgtttccgtg gaaaaaacgg
     8461 gcacgccttt tcccatgcac tccgccacgc tggcgcgggc ctgctctgca ggaagcactg
     8521 ccggcacatc cgtcagaacg ccgttttcca gcctccccca cgtccagccg ccacgccggg
     8581 catctgacat tacgcaggcc tcaccatgat gcagaccaag tccgttccag gaggaaaaag
     8641 ccgccacgcg ggaaccgtgc accagggaaa ggccgtccgc cacggccagc gctacgcgga
     8701 ttccgccgta agcgcccgga ccggaaccta ccacgatttc cttcaaacgc cgcccttcca
     8761 aagcatccag aacctgccgc accgccttga aaatagcgga ggaatggttg cgctccgctc
     8821 tccatgccgc ctggcacagc agactttccc cgctccaagc cgcaacggaa gactgcacac
     8881 aggaagtttc cagaaccagc aggacatctt gcatgcggcg gattatacag gaacagccgg
     8941 cgtggataaa acattttttc ctccggtttc cggaagaggc aaatttggtt cgcatttctc
     9001 ctcaagtccc tttatgctgg ttccagacct cacaccgcat gcccaaggcc cccagacaca
     9061 caccgccgcg ccaggactgg cgccggtatg cagaaaccgc ccggaaacca agatccgcgg
     9121 caagcctcgg cgacatgatg cggcaatgca accgggacgc ctgggacaaa acttaccgcg
     9181 cccaggtatc ctccgaatac tgggaggacg aatgccagga attttccttc atggatctcc
     9241 cgcgcagaac ccggttcatc aggcatgccg tagccaccct tatcctgctt ccgctggccc
     9301 tgatcacggc catgtccctg tgcgaccagc tggcccatgc ctccggcagc ctcaatatcc
     9361 tcttctccat tcccgtatgg tacgccctga tgggcgctgc cgtgtgggta gtgctgggca
     9421 gcagcaggct gttcacatcc tccctgctgt acctgtacgt attggggcat gaactgacgc
     9481 acgctctggc ggtcatttgc agccgcggct ccatccatgc tttcaaagcg gatctggacg
     9541 gaggttacat ccagacggat aaaaacaaca tctttatctc cctttccccc tacttccttc
     9601 ctctctgggc catgctgtgg ggattggtat ggggcgtaac gtacctcttc tggcctacag
     9661 tcacctgcca ggcccttctt tactccggcc tgggcttctg gtggtgcttc cacctgttct
     9721 ggacggcatg gatcattccc aaggaccagc cggacctggc ggacaacggc accttcttct
     9781 ccctgattct cgtatatttt gccaatcttc ttctcttcct ggcgctcctg cgcctgttca
     9841 atgccgtaag ccttcaccgc tttctggcgg attgcatcca caatgcggaa aaattgtggg
     9901 acgttctcag ggacctcgcc atgctggctg ccgggctgtt cctgtaaaaa atattctttt
     9961 ccttacacgg aatgatgaac ggcagaaggg gaaaaggaat ccaacctttc aacagaaagg
    10021 agtttccatg cgcaccacac acttgatcat tctgacaatg gccgcagttt ccctgatggg
    10081 ctgcaatccc aaggaaactg taaacgccct cacttccgac cagaacgtta cccagaagat
    10141 ggccgtaaga ttcgcggaaa atatggttgg cctgccacgc gctctggtcg tttcagatgc
    10201 cagaaaagcg gctccgggcg acaaagtcac cgtcaaggga gcggtaatcg gagatgaccc
    10261 cgtcttttca aaaaacattg cttccttcac catcggagac ccggctcagc tcctggtcaa
    10321 tatgaaggag aagggagtct gggccgccgg tacggtaccg gtggaaatac ggaaaaataa
    10381 cacgatgacc gtccagttcg tggatgaaca ggggcagccc atcaagcact ccgccaaagg
    10441 tgtgaacggg ctgaaagaac aagacgccgt catcattacc ggaaccattg atccccactc
    10501 cactccagaa catccggtgc tgaacattga aagcatcgtg attgagtaac cccttctgtg
    10561 ccaaaccctg attcatgtca aacggttcag gcgttttgcg cctgaaccgt ttttcatatc
    10621 agaatgcgcg catgggattt gatccagcat cattgggaat gataacactg gccgctactt
    10681 cagcggcagc aggtgcggca tccgcgtctg cacagaaaaa aagcgcggaa gccactagac
    10741 aggcggcgga cgcaaatgcc gccaccctca ggaacgaggc agacctccaa aaacagacag
    10801 ggatggaaaa tgccaaacgc atgcagcagg acgctgcgcg ccaaatggga gaagcccgac
    10861 tggacgccgc ccaatcctcc ctgctccggg aaggctccgt tccaatcagg gaacaggctt
    10921 tggcgacgcg tctgcaaacc aggattcagg atgaagcggc ggaagccatg aggaacgccc
    10981 aatccctgta caaccgcgcc aacacggagc aatacatggg ccgctccaat gcgagagccc
    11041 agaaaacctc agcaatgggc accctgctct ccggattcgc ccagggagga aataccctcc
    11101 tcaaatggtg aagtgcctca caaggcacct ccattcaaac gaagacagcc ggaaattcag
    11161 ccgcaaccgc ggaagcttct taacgccccc ccctgccggg ctgccccgga agagcagcct
    11221 cccaaaacag ccttacctga cacgtccttg cctgtcttcc tcacatcacg ggtcataagg
    11281 ccagcacact tccgcttacc cgcgcctcca gggcaagcaa ttcccgtttc gcatcaagcc
    11341 ccccgccata acctgtcagt ttctgattgc tgccaataac gcggtggcag ggcacgaaaa
    11401 tggagatggg gttggtggca ttagcggacg ctacagcccg gacagccctc ggatgatgaa
    11461 tgcggcgcgc cagctctgca taagaaatgg tggaaccgta aggaactttc atcagttcat
    11521 gccagactga gttttgaaat tccgatccgg taaatacaat gggtatatca aagtctctgc
    11581 gttccccctg gaaatattca cggagctggc ttaccgcctg ttcaataacc tcggaagttc
    11641 cttcttcata ttcagcgttg agatgccggc aaatgcggcg gtctatcgtg ccgcggcgtt
    11701 tttcctttgc ccagtcgcaa atgcaaagat tgccgccata tgagccaatc atcatttctc
    11761 caacgggcga cagataacgg attattttaa ttgcctccat cttcttgtcc ttgttattgc
    11821 ataacgctct ttcatccatc ccctgtacgg ctgggaaaca ttgctccatg cctgcctgtt
    11881 catgtcccga agcacaatgt tccgccccaa aggctcttct ccggcagcct ttagcacatt
    11941 tccttgctat ctgcaatctt tcttccgcat ttgctttttg tttccaatcg gacgtgaatc
    12001 ccaagttatt cgccctctcc ggccggacac cgataatccg gatacctgct tttcccttga
    12061 atcattcccc attcaatcaa ccatgacgat acctcgcaca agagaggcaa aatccctgcg
    12121 tttttaatgc tgatgcaagg ccctgaataa tattgggtgg cgtccccggc aggaatcgaa
    12181 cctgcatcta gtctttagga gagacccgtt ctatccattg aactacgggg accggacatg
    12241 gcccgtgcat gacaataaac gggaaggcct ccgcgctcaa gcaaaaagca ggaggagtat
    12301 gccatgcatc gtgattttga acagtattca ccaggataat ttttacggat ttctgaaact
    12361 tcccgtttcc cctcctttct ccgcgcatcc ctggccgaat acggccattg ttccgattac
    12421 ctgccggaag cggcccagaa taatgaaata accgtcttcc gctggcccct cggggccgga
    12481 aggcgccgcc actcctgcag agagtcattt ccgctgaatc ttatcgccaa aaaacaacgg
    12541 aaagaaaaca atcctccaga aagctccgtc cagatgannn nnnnnnngcc aatgatgaaa
    12601 aaacagagaa gaacagaacc cctgcatgag agattatcct ccggaacagc atcccccctg
    12661 gaaaggggaa caggccatac aaaaatcttg agaagggggc ggaagggccg tagcgttgcc
    12721 gtatcattcc cgccatgctc aacttcgttt acgataacaa aaccactctt attttcggaa
    12781 aaggcaccca ccatgaaacg ggaacccttc tcaagccgtt aggcaaaaaa atcctgcttc
    12841 attacggtag cggcagcatc agacgttccg gtctttacga tgccgtcacg gcatccttaa
    12901 aagccgccgg cgtggagtat gaggaactgg gaggcgtcca ggcaaaccct acgctgcccc
    12961 tggtgtatga aggcatccgc ctctgccggg aacacggcct gggcctcgtg ctggccgtgg
    13021 gaggaggcag cgtgattgac tctgccaaag cgattgcgct gggcgttccc catgaagggg
    13081 acgtatggga actatacctt tccaaaaaac agcctcagtc cgaccctctg cccgtcgcaa
    13141 ccgtgctgac cattccagcc gcgggcagcg agagcagccc gaatacggtc atcaccaatg
    13201 aagagaccag aagaaaactg gggtacggtt cccccaaact gcgccccgta ttcagcatta
    13261 tcaacccgga attgttcttt acgctcccgc accgccagat ggccaatggc atcagcgaca
    13321 tgatgagcca catttttgag cgctatttca ccaaaaccct gcatacggac ctttccgaca
    13381 gcctttgtga ggccaccctg cgcaccatca tgaaaaacgc ccgcatcctg aacgggaacc
    13441 tgaatgatta caacgcctgg gcggaaatcg cgttttccgg caacatcgcc cacaacaacc
    13501 tgttgggggt aggccgcgaa caggactggg gctgccacgc catggaacac gaattgagcg
    13561 cactgtacca tgtggaccac ggagcaggcc tggccgtcgt caccccggca tggatgaagt
    13621 atgtttcccc caaacaccag gaaatattcg tgcagttcgc cgtcaatgtg atgggagtgg
    13681 aaggttcctt ccgggaaccg tcagccatca tccgggaagg tatttcccgt ctggaacgtt
    13741 tttaccggga attagggctt ccgacgacta tggaagaact gaacatcctt cccggggatt
    13801 tccccctgat ggcggaacag gccgtcagtg tgcgcggnnn nnnnnnnttg cggagggtaa
    13861 aaattcccct gtttccccgc agcaaaggaa gcaagctgtt gaaaaccttg aaaagcttct
    13921 gggcaaaagg ctgttcaaga aaacggcgga acaggccctg aaacaactcc atgaatatga
    13981 cgaccaatac agcgggacag atctgcttct ttactaccag gctcttaccc gtctcaatgc
    14041 cctggatgat tccatcaatc tggattccat tctgcaggaa cagatgaagc gccacggcgc
    14101 caatccccat ttcctgaggg acgccgcact cctgtaccag agtgcctgcc atacgttcaa
    14161 actggtggac ggcgcctata tccgcggcgc cgggccatgg gatggagaat attccggaga
    14221 agcaagggac cgtgtggaag ccctgcggtg ccttgtcaaa gccatgcagc ttgcggagaa
    14281 gggcaacaac atgaaacttc tgggacaact gcgcttcatc acggcacagg cgttgccggt
    14341 caaaggaaac cgctacgctc ccctttccgc cttccagtct tacgcagccc tggggaacct
    14401 gaccaacctg aaggaattgc cggattacgt ctccagagag gaagcatccc cttttcgaaa
    14461 cgtcgccacc gttcccgtca tggtcaatgc cagaacagga aagccggaag ttatctttta
    14521 ccatgcctct tcctcctggg aaacagctaa aaacgacggg gaacgcatgc gctggctgct
    14581 ggacgccgcc attcaggtga atccggaact ggccaaccag gtcaattatt tcaccgcctc
    14641 ctggtgccgc cggctgttct cttatgccaa tacggcgccg gaccaggaat tcgtgtacgg
    14701 ccccggcaac gcgggcatgg tggcgggcat caacccggcg gaattaaaaa cggaccagac
    14761 catcgttaaa accgaccgga cgggcaacgg taaattcctg ctcaccaacc ttcccccgga
    14821 ttatgacttc atccggattg cttccgccgt ccgaataacg ccggagccgg attactacgt
    14881 taacgccgcc aatctggcag cggacgaatt cctggcccgc aaccaaaggc ccgctgccgc
    14941 gcagttttta ggaaaaatcc ttcaaacctg gaacgcacaa tcctggaaca aaaaggacaa
    15001 ggaggaattt ctggacgtgg cggacaatct gaaaaaacgc atcgcctcca tcacggaacc
    15061 caacggaaca tttgatacgg ataaaagaac gctgctggcg ggagaaccgg tgactgtttc
    15121 cttctcctac cgcaacgctt cccgggcctg cgtagcggtc cgccccgtgg atatgaaacg
    15181 gtggcaggaa gaacgcatgg acagggtcca aacctccaaa acgctgggaa aagcttacaa
    15241 agacaggtat tccaacctgg ggaacctgct cttcagcctg ttgcatgact catcctatgc
    15301 ccgctatctg ggagaggaaa tcaaggggga tgagataacc ctgactcccg ggaaccggca
    15361 cctgaaccgc atcgcacaca ttcccgtacc cacccgcaaa cccggctggt acctgctcac
    15421 cgtcacactg gaaaacggct accggttcca tcgctttctc actctgtcag acatggtgct
    15481 ggtacgccgt tccgttccgg aaggcaatct ctggttcctg gcggacgcca gaacgggaat
    15541 gcccgtggaa ggaggcagcc tgcggctcct ccgctaccgg caggataaaa cgctccaaaa
    15601 acgccaggta aagggaatca cggacaagga cggagccatg acagaaacaa tccctcaccg
    15661 ccattccagc gcccctccct acaacatgtt cctgggcatc gtttccaagg gggacagtta
    15721 tgtgctggcg ggaatatctg actacggctg ggaatccggc aattacctca tggatacggc
    15781 caacaaggcc tccgactcct attcctgctt tttcctggaa agccagcccg tgtaccgccc
    15841 cggacagacg gcccgtttca aaggcgtcct cttccgcccg gacatcgcga atcccggaac
    15901 aaaagactgc gccgggaaaa aactgacgct gaccattgcc tctccaacag gggatgagag
    15961 cattttccct ccaaagaccg tcacgacgga ttccacaggc agctttgaac tggaactgct
    16021 cattcccgcg gacgccccgc tgggccagta tggggccaag cnnnnnnnnn naagctgaaa
    16081 ctgcatgact ccgcagatcc ggatttccgg ctgtatgccc cccttttccg gatggaagag
    16141 tataaaaagc cggaagtctc cgtcagaatg gatgcccccg ccaaacctat ccggctggga
    16201 caacccattc ccgtcgccat tcaggcggat tattacgcgg gcggccccgt aagcggggga
    16261 aaggcaacca tcaccatcac ccggacgctg ggggcgaaca tctggacgcc ttactggaaa
    16321 tggagctggc tgtacgacag gtctttcagc ccgtactaca ttcccttttt cccggatgcg
    16381 cctctgaccg tcctggaaaa gacggtgccg ctggacaagg acggaaaagc atccgtagaa
    16441 ttacccacgg cgcaggatgc ccgggatttc ccctccagca acgtcaccta ccgcgtttcc
    16501 gtcagcgtga cggacgcctc cctgcgggaa gtttccgctt ccggacaagt cattgccact
    16561 ttccgtccgt ttaatatctt catcaccctg aaccgcggct atgcccccgc cggaacgccg
    16621 gtccaggcat ccattaccgc agccacggcg gatggcgcca aaattgccca tgccagggga
    16681 acctgcgtcc tgcagcatat ccgtgcggac ggcagacggg aaacgctgga aacctgggat
    16741 atcgccaccg ggaaagatgg ggaagcctcc ctgtccttcc agaccgggga atccggcctg
    16801 tattcccttt ccacaaccct ggaggacggg cacggcaaca aggtggaaga atccttccag
    16861 ttcctttcct acggcaaggg caaacagaat ccttttaaaa tcaatcctct ttccatccat
    16921 ccggacaaaa aggaatacgc tccgggagat acggccaggc tgctcgtcac ctctgactat
    16981 ccggacgccc gcgtctggac cttcctgcgc aactcctgga aaaacgaatc ccgccgtctg
    17041 gtttccctgg accggcagac agcccttgtg gaatgccgcc tgacccggga agatatgccc
    17101 aatatgggag tcaacgcctt caccgtcaga aacggagaac ttcatgaggc ttccgccgaa
    17161 cttctgattc cgcccgccgg acagcttctg gctccttccg tagtgccggg caaatcccaa
    17221 taccggcccg gtgaacaagg caacgtcacc attcaggtca aggggcctga cggcaaaccc
    17281 gtcagtaacg gcatcgtcgc cctggccgtt tatgacaagg ctctggaata catcgcacgc
    17341 cccaatatta cggacatctc caaaaccgta tggggcagac tgaacgaaac tggcttcctc
    17401 agcctgaaaa aaatgacggc ttccggcacg cagcaggatc gcggtccggg acagccctct
    17461 ttccaatccc tgctctacan nnnnnnnnnt gctctacaga aattacggac ctatggcgag
    17521 aaaggccaaa gggaccgtta acggctttgc ggaggcagtg ttcgattccg gtgctgatgc
    17581 tgccgcaagc cgcgcattgg ctacaggagc ggccgccccc gccgccgttc cggtcatggc
    17641 catggccgcg gacaaagaaa gtgcagaaag cgagtccctt gcaaacggac agggaaatgc
    17701 ggacgcgcag gaaaacggtt ccccgcacat tcaactgcgc accaattttg cggactgcat
    17761 caagtggtgc ggcacgctca aaacggatga agaaggaaac gttgccgtac ccgtggaaat
    17821 gccggacaac ctgaccacat ggaaggcctc agcctgggtc atcacccccg gattgcaggt
    17881 tgggcaggct tccgcagaat tcctgaccac gaaggacttc atggtcagca tgcaggcccc
    17941 gcgcttcttt gtggaaaaag acatcgtgat gctcagcgcc ctggtccgca accggacggg
    18001 aaaggctgtc cgcgcccgcg tttccatttc cctgaaagac gggtgcctgg aacttctccc
    18061 ggctgacgat ccggccgtga aggggctttc cgcagatacg gataattccg ccgtgcggga
    18121 agtggacgtt ccnnnnnnnn nntcaactgg tgggctgccg ccgtcaggga gggaaccgcc
    18181 gccgtcgcca tggaagcatc ggctggctct accggagacg ccatgcagat gaacttcccc
    18241 gtactggtgc acggcatgaa gcagctccat gcggaaagcg ccgcagtgct ctccggagaa
    18301 caggaaaaga cactttccat ttccctgccg cagcagcggc gcagggagga aagcgaactg
    18361 gtcgtcaagg tctcccccag catcgccctc agcatggtgg aggccctgcc ctatctggcg
    18421 gaatacccct atggctgcgt ggaacagacg ctcaaccgct tcctccccgc cctcgtcgtg
    18481 acggacacgc tcaagcagct cggcctgaat cccggagcgg cgctgaaaag ccatcgcagc
    18541 ctgaatcccc gggacatcag gaacaaggcc ttccatgaca gcgtcatgaa aaaactggaa
    18601 cgcaaccccg tttatgatga agccgcgctg aaaaagatgg ctgccagggg aatctcctcc
    18661 ctccgggaaa aacagctctc caacggctcc tggggatggt tcggaggggc ggaagaggga
    18721 gatcccgtca tgacggccca cgtggctcac ggcctgaaaa ttgcttcaaa cacggtcaac
    18781 gtgccggaag gcatgctttc cggcgctgtc cgctggctga aaaactatca ggaacgccag
    18841 acagctcttc ttgaacaagg agacaaattc cggaaactgg aacaactgcc ggacggacct
    18901 gaaaagaagg aagccctccg caaactgggg aattaccgcc tgacggcctc cgcgacggat
    18961 accctggtct actccgtcct ggcggaatgc ggcgtgaaaa acctgcctat ggaacgctac
    19021 ctgttccgtg acaggctgga acttcccgtc atcagccaga ttcaactggc ggaaatcctg
    19081 ctggacgccc accgcatgga cgacttcaac aaggtaatgc ctgtcatctc ccaattcctc
    19141 cagcaggacg attccctgca aaccgcctgg ctacgccttc ccaatgcagg atactggtgg
    19201 cgctggtacg gcagcagcgc agccacgcag gcggcctacc tgaaactgat gtccaagagc
    19261 gcccccggca atcccgtcac ggcgcgcctg gcaaaatggc tgctcgacaa ccgcgccaac
    19321 ggttcctact gggactccac caaggatacg gcggattgcc tggaagccct gtccgcctac
    19381 ctcctccaga ccagggaagg catggaggac atggaagcgg aaatccttta tgacggcgtt
    19441 ccggttaaaa cggtttcctg cacgaaggaa acactgttta cctttgacaa tgctttccgc
    19501 atgagcggga aagccctggc ggacggcagc cacgtcatca ccatccgcag gaaaaaaggc
    19561 agcgggaaca tctatgccaa ttccacgctc agctacttct ccctggaaga ccccatcccc
    19621 gccgctggca atgccgtcac cgtggaacgc tcctattacc gcatccgaaa ggaaacggtg
    19681 aaaaacggct ccgtgaagga cacccagacg gatgcaggag agctcgtctc ccagggcaga
    19741 gacctgacac ggcgcacgct gttgaaaaac ggggacgtca tcgccagcgg cgacatcatt
    19801 gaagtggtga tgaccgtgaa aacgaagaac gacgtggaat acctcatgct cctggatccc
    19861 aagcccgcag gctgcgaatc ccgggaaacc gccagcggct acgcccggct gggaaccgtc
    19921 ttcggctaca gggaaatagg ggatgaagaa atacgcctct tcctttcctc cctgcccatg
    19981 ggccaatacc agatcagcca ccgcctgcgc gcggaacgtc cgggacgctt cagcgccctt
    20041 ccggccgtca ttgaggccat gtacgccccg gaactccgcg gcaacagcag ggaacataaa
    20101 atcggcattt ccatgcctgt tgaacagaat aatgaccagc cgcagtagcg ctgggcaaaa
    20161 acgccacgat caacacatat ccgaaaaagg gcaggcatca acccaagggg gagttatttt
    20221 tgtacctcat cttcaatcca cagcaaggct tgggcaagga aacaaaaatg aactttaacg
    20281 acggaaacaa tatggagtcg ttattttcca ttcaggagaa aagccggaaa cgacggggaa
    20341 agatgagtca gaataacgag tccttccaaa tgctccggat agacacgttt cccctgtatt
    20401 ccacgctcct tcccgcacta aaaactcttg caatccgctt gcggaagcgg agaatgtgcg
    20461 acccgccgcg cagcgggccg caccatgcca tcagaagcgc tgaaaggaca tatcgcgatg
    20521 ggagcggcgg ccgtcatctg gggcctcatg tctcccgtca gtaaactggt catgcagcag
    20581 ggagaagtaa gttccgcctc tctcgccaca ttccgcctgc tgggagcggc cctcctgttc
    20641 tggctggcct ccgctttcgt gccaagggaa cccataaaga ggaagcatcg gctgcctctg
    20701 ttttacgcct ccctgttcgg aatcattttc aaccagatgg ccttcacggt gggcgtaggg
    20761 ttcacttccc cggcggatgc ggccatcatc accaccatta caccggtatt aaccatgatt
    20821 ctggcggtgt tcgtcctccg ggaaatggtc acggggaaaa aaatcatcgg ggtctgcgcc
    20881 agcgccatcg gagccatgtt actggtttcc ggcagcggcg cgcatgcggc ggccctaccg
    20941 ggagacaaca acctgctggg ggacattctc tgcctggctt cccagtgcag cgtagccgtt
    21001 tactttgttt tttttaagaa cctgattggc agatactctc ccgtcaccct gctgaaatgg
    21061 atgtttacgt atgccgcagc cgcctgcctc cccttcacct tccgggaagt gggttccatc
    21121 cattattccg ctctgcccgt acaaacatgg ctgggcattg cctatgtggt ggctctggct
    21181 acttttgtca gctatatctg cctgtctttc gcccagcaga ggctgaaacc caccgccgtc
    21241 agcatgtaca attactgcca gccggtgatc gcatcatccg tagcggtgat gtggggcatg
    21301 gaccacttcg gatggatgaa agtcctctcc gtcctgttgg tcttcacagg tgtgcttctg
    21361 gtcaccggaa ccaggggaaa acgggccgtc caggaacaat aaacacgcca ttcctccgaa
    21421 tttccg
//
LOCUS       DAWEMX010000031        21071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1051_length_21071_cov_13.388514, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000031 DAWEMX010000000
VERSION     DAWEMX010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 21071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21071
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1051_length_21071_cov_13.388514"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1022..1031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19730..19739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20523..20532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4824 a         5069 c         6011 g         5137 t
ORIGIN      
        1 tttccactgg ctggaccatt atgccgccag ccagcagccc ctggcccatt acgttgaaaa
       61 aatgaaggaa tgggagaaga atcatggtgt tgcggtaaca ttccatcttt tgcctcatga
      121 tgctgcacgg agggatgcgc acggcatttc ctatgtggag aatatgggga ggctgggatt
      181 ggtgaatgtg cgcgtcgtcc ccagaaccac ggatgtctgg cgtggcatta atacgctgag
      241 ggaattgctg gagcgcagtt ttttccatgc ccgcacccag gaaaaggcgc gaggcttctg
      301 tggggaagag gagccgggag gcgtggagca tctggagttg tacaggtcca ggcctcccgg
      361 cgtaggcgga gccattgcgg aaagccccgt gcatgacggg cattcccata cggcggatgc
      421 cgcacgaacc tttgcggagg cttggtccca tggcctgctc cacggaaccg ggaacggccg
      481 ggagcgcggc aggcgcgccc ggatgtggta acggagggca ggagtaaagg aaaagagatt
      541 tttttgattg atagttgaca gttttgtcaa aaagcggttc tttttttcgg cctccagctg
      601 tctaacgcag tgatgcgact tattgagatg aaattatccc ttttccatcc gttggtttcc
      661 tgcctgtgtg ttccgttttt gttttcgtcc tgcggtacca cgggaaggga gccttcccct
      721 gtcccctctt cccatattcc ggtagaagaa atgacggggt attttgaaga aacgggaact
      781 attccggggc acctgctcaa gtgggaagat gatccgtcgc tgcccggaaa gctgctggtc
      841 gtagtggata agaaaaagca gatgatgtat atctaccggg ggacgcaccg cattgcgtat
      901 gcccccatca gttccggaag aagttccggc atgacgccct ccggctactt cctcattgct
      961 tccaaggagg aagaccatca ttcctattac ggtgcgttta ttaacgctga cggagaattg
     1021 cnnnnnnnnn ntatacgaag ggattccccc cgccccggcg agaggtttga acctgccaag
     1081 atgccctatt tcatgagagt gaacggggct gtaggaattc atgaagggta cttgcccggt
     1141 catccatcct cccatggatg tatccgtgtt ccccatttga ttgcgaaaaa tctctttgaa
     1201 atagctcccg taggaacccg tgtggttgtc agggatggaa actggaatat tcacgagctt
     1261 caacaaaagc cgtccggaat attccggacg gtgcacaagc ctgctgcccc ggtggctggc
     1321 gcaaattcag cttcctccgg aaagaaggaa cttgtaaaat cggaaacgtc cgtaaaaaaa
     1381 gatgccgccg ccataccgtc tgttggagaa cagaaaaacg ggcggctgcc tttttctgag
     1441 gcggacgctg tgaccccctc ttcatccaat ggcctcgaag gactggaata acatgctgtc
     1501 cgggcgttta tatcatgccc aggattctga attggctgcc gcccggctca gggcgcgccg
     1561 gcttgttttc cgttataacc agtccgctcc ggaaaatgaa agcctgcgcc gggaattggc
     1621 tcgggagctt ttcggccaaa tgggagaggg gtgttatctg gaacctccac tgcgttgtga
     1681 ttacggctcc aatatacggt tgggggaccg tgtgtatgcc aatttcaacc tcgtggtgct
     1741 ggactgcgct gccgttacca tcggcaatga cgtgctgata ggtcccaacg tgggcattta
     1801 tactgccggg catcccgtag acccggggct gagacggcag gggctggaat ttgctctgcc
     1861 cattaccatt gaggatggag tatggatagg cggtcatgcg gtgattgccc cgggcgtgcg
     1921 tattgggaga aattccgtga taggcgcggg cagcgtggtg acgaaggaca tcccctccaa
     1981 tgtagtggcc gtgggtaatc cctgccgtgt ggttcgctcc gtgacggaaa aggaccgcga
     2041 agtctgggac accggggaaa atggagaggg gccgctttcc cagggaccga aggcaaattc
     2101 cccgggatga cgaaatttga attgcttttg gggctggatg atgcatagtt ggcgccctca
     2161 cagtgtcgat tagccgctgc cacgagctaa atacattcaa ggaaaacact tatgagtaac
     2221 gaaatcaatt tgcaggaatt caagatgcat gaaaaggatt ccggtagttc cagctaccag
     2281 attgcccgcc tgacccagcg catcgcccac ctgaccgaac acctgagcac gaataaacat
     2341 gatgtttctt cccgccgcgg gttgttgaag atggtggctc tgcgccgcaa gcttcttgat
     2401 tacgtcaagc gtgaagatct ggcccagtac cagagtctga tccagcgtct tggccttcgc
     2461 cgctaaaagg cggagcatat tcccgtttga ttcggccgtt gcggttttcc gcagcggccg
     2521 ttgtttttat gggaggggct ggcgtggtag cgttgtcatg gaagggggga tatggcgggt
     2581 gactgtggat ttgtgttttg gtcaggagag tttgaaaggc ctgtcttttt gtgaaagaag
     2641 atattctgga gtgtggagat tgtttttgtt ggcatgactg cccctttatt cctgcgtaaa
     2701 tgtttgctgg ggacggaaag tccggtattg cgggtatgat gcagctgcgc aaagaatagg
     2761 aaaatatgga tgaaaaagca gtggacggct gttatgggag tcaggcagga atactcagtc
     2821 cgtccttaaa ttctacattt ccgctcctgt gaagaaggga aggcggacgt ttccggatat
     2881 tttgcttgga gtaatgcatt tcaatcagga atgcgaagaa tattgaatgg atgaaggtga
     2941 gatgggaaaa ataccttttc tccccatgcg gtggatattt aaagccctgg aaggaaatgg
     3001 actgctatgg ttatcaaact atttgcttgc attatccttg acagggatta tgctctcatt
     3061 cgacgttcgg ggattcgttt cccgaaacgt ttctgtcggg catgccttga gaaattttcg
     3121 gattttcaca ggctgtcaag ccggtaagac tgggccggag cgtttgatcc atctgggatg
     3181 cgcgtcctgc cgcgtggaca gtattccccc ctctgtttct gggaaaggac tgtcgcggat
     3241 aacatcttcc tgaggcactg cccgtatctg acgccattca ggcttcccgc ccggagccgg
     3301 cctggatggg ctgatgaggc tgcgggtgat tgaaagacaa aaaatataat gagcatacat
     3361 tcagttgaat gcaacgttgg tacgaatcct atcacgattg aaaccggcaa gatggcccgt
     3421 ttggcggacg gtgctgttgt tgtccgcagc ggcgataccg tcgttcttgt gaccgtggta
     3481 agcgccacca aagtcaagga agggcaaacc ttcttccccc tgtccgtgga gtacaaggaa
     3541 aaggccgctg cggcgggtat gtttcccggc ggctatttca aacgcgaagg gcgtcccacg
     3601 gaaaaggaaa tcctgacgtg ccgcatgacg gaccgccccc tgcgcccgat gttccccaag
     3661 ggctacttct acgatacgca ggtaattacg cttctgctct ccgccgatgg tgaaaacgaa
     3721 ccggacatcc tgagcattaa cggcgcttcc gccgcctgtg tcgtttccga tcttcccttt
     3781 gccgaacccg taggggctgt gcgtgtgggc cgtattgacg gacagtttgt tatcaatccg
     3841 accaactccc agcgtgaaca tagtcagctg gatctggtat ttgccggcac gaaggatcag
     3901 gtcatcatga ttgaaggttc cgctaatgaa ctgccggaag aagattttat cgccgctttg
     3961 cgtgtcgccc aggagaacgt gaaggtcatt tgcgaaaagc aggaagagct ccgcgccgtt
     4021 tgcggtaagg agaagcgcgc ttatgaactt tgccttgcca agccggaatt gctggaaatc
     4081 ggctatgaaa ttgccggcga tcgtattgag gaagccattt atgccccttc caaggtggag
     4141 cgtcagaaaa aagtgggggc cctgcgtgac gaagtggagg ccgccatcaa ggaacgccat
     4201 ccggaagcta ctgactttga tgtggaacag gtttttgaat atattcagaa aaaggctttc
     4261 cgcatctcca tcatggaaaa ggacaagcgg gccgatggcc gggctctcaa gcagctgcgc
     4321 cctctgactg cggaagtcaa tgtgctgcct cctgtggtgc acggttccgc gatgttcgcc
     4381 cgcggtgaaa cgatgtccct ttgcctggcg acgctcgctc cgatggaaga acgccagtac
     4441 atggacaatt acacgggcag cgtgaatgaa aagcgtttca tcctgcacta caatttcccg
     4501 cctttctccg tgggagacac cggccgtttc ggcggtcaga accgccggga aatcggccac
     4561 ggcgctttgg cggaacgttc catcgctccg gtcgtgcctg gcgaacagga attcccgtac
     4621 gccattcgcg tctcttctga aatcatggaa tccaacggtt ccacctccat ggcttccgtc
     4681 tgtgcgggca cgatgtctct gttggcggct ggcgtgcccc tcaagcgccc tgtagcgggt
     4741 atttccgtgg gcctggtgac ggaacagaat gaccagcatg aaatcacctc ttacaaaact
     4801 ctgctggata tcatcggttc cgaagacttt tacggtgata tggacttcaa gctctgcggc
     4861 acgtcggaag gcgtgacagg ctaccagctg gacctcaagc tgcccggcat tcccctctcc
     4921 atattggagg aagccattca cgtggcgaaa accggccgca cggatgttct gaaggtcatg
     4981 aatgaagcca ttgcagctcc ggcccagatg agcccgaatg ctccccgtat tgagaccacc
     5041 aaaattcccg ccgaccgcat tggcgaactg atcggccccg gcggcaagaa cattaaggct
     5101 attcaggccg aatccggcgc cgatatcaat atcgaggaag atggcaccgt gcatatttac
     5161 gccgccaagc aggaaggcct ggaccgtgct ctggaattgg tcacccgcat gttcaagacc
     5221 attgaaatcg gggaactgta caccggtaag attgtttcca cgaccacctt tggagcgttt
     5281 atggaagtgc tgcccggcaa ggatggcctg attcatatct ccgaactggc cgaaggccgc
     5341 accgccaaga cggaggacgt cgtgagcgtc ggagacgtgg tgaccgccaa gtgcatcggt
     5401 attgacgaca aaggacgcgt gaaaatgtcc atacgtgccg ctttgcgcga tgccaaggct
     5461 gccgaagcag aagccgccgg cattacggaa taacgttttt cgttgagcct ttaaactctc
     5521 agccccgttt ccggttattc cgggaacggg gctttccggt tgctggaaag atgttttgca
     5581 gttggttcaa ctgcttgcgt gatcagccgg aattttttta cctgtgccgg aaggagaaag
     5641 tttggaatgc tggaaaggga cagaaggaat ggagctcttt ccgcatggca gatttttttg
     5701 tttcccgttc ttgacaggat ttttccggtt cctatgatgc ggatgcccgt tgcgttttac
     5761 ggacacccct gccatacatg attctttttg tcactttcgt tattcttttt ctgattctgg
     5821 tcaacgcctt ttatgttgca gcagaattcg ccgcagtgag cgtacgccgc aacatgatcc
     5881 gggaaatggc ggagaacggc agcagggtgg ctgttcatct gcttaagatt ttggaaaata
     5941 caaaagagct ggaccgctac attgcggctt gccagttcgg cattaccatt tccagtctgg
     6001 ttctgggcgc atacggacag gttgaactgg ccgcctatct gttccctctg tttgagcggt
     6061 ttggtgggat ggactccgta atggccaatt ctgcagccgc tcttgttgta ctgataggct
     6121 tgacggtttt tcaggtaatt cttggagaat tgatgcccaa atccctggct cttcagtttc
     6181 ccaagcaggc ggccttgtat acttattatc ccatgcgatg gacactggct ttcttcgcat
     6241 ggttcattga ctttcttaat ggaagcggtt tgttgcttct taaactgttc cggctcccac
     6301 ctggcggcca tcagcacatc cactcccagc aggagattaa tatgctgctg gatgaaagcc
     6361 accagggcgg gatgttggag gaagatgagc atgaacggct gcacagcgct ttgtccctgg
     6421 cggaacgaac ggtggaacaa attatgattc cccgttttca gcttgtttgc ctggatgtgg
     6481 atgccacaca ggaagatatt ttgaatatga tcgcagatag gcctcatacc catattcccg
     6541 tgtatgaagg gaaccgtgaa tctgtcattg gcatgctgca tattaaagat atggtatccg
     6601 cttatgcgga aaagggaatt ctgcctcctc tccgttccat gctgaggcag gtgccatgcg
     6661 tgatggaaat gcagacggta gagatgctga tggcccgttt gcgggaagac agggccaagg
     6721 aagcctttgt tttggatgaa tacggtaagt ttgtggggct ggtgacgctg gaacgtctgc
     6781 tgggagaaat ggtgggagat atagatgagg aattcatccg ctccggggaa aaggtggaaa
     6841 cccttccgga tggttccgtg cgcatccccg gcatgatgcg tgcccataag gcggaatgcc
     6901 tggtaccctt tttgatgaat ggcgccacta ctgtgggcgg ctgcgtgatc aagcacatgt
     6961 cctgcattcc gaaggatggg gaccgcctga ttatcgccgg acgcgtgctt gtggtggaaa
     7021 agatggacca taaccgtgtt tcctctatcc tgctgctgcc tccggagaga aaggaaaatg
     7081 aatttgccat ggaaagcggg gtggatgcat gatgacggtt ttcattattc tgttgctgat
     7141 tgtgctgaac ggcctgtttg tggctgcaga atttgcgttg ctcggcgctc cgcgtgcggc
     7201 attggagcag atgggtgtgt ccggcaacat ggtggcgcgc cgcgtttccc agatattgaa
     7261 aacgccccgg ttacaggacc gctacattgc tacggcgcag cttggcgtca cgttcgccag
     7321 tctggggttg ggtatgtacg gagagcatgc ggtagccgga tggctccatg aatggttggc
     7381 gcagtacggc tgggcttcct ggctggtggc tcatggcgca gccagcatcc tgtccgtggc
     7441 ggtgctgacg tacctgcata ttgttcttgg ggaaatggtg cccaaggcgc tttccctaca
     7501 gtacgccgca aagctttgcc tcaccattgc gatgcccatg tacgtgatcc agctatgtct
     7561 gtatccgctg gtagtgggta tgaatgccct gggcaatttc tttctggggt tgctgggtgt
     7621 ggaccgtaat gaatccaagt cccactacca ttctgcggaa gaactccagt atattattga
     7681 agagagccat gagaacgggg cgttgccgca ggaatccggc cgcatcatgg acggcttgtt
     7741 tgacctggat gatctgtatg tacaccaggt catgaccccg cgcgtcagga tagacgccat
     7801 tccagaaggg gcggaacatg agcagatacg ggaaattgtg cgtcgcactc gccgtacgcg
     7861 ttaccctgtc taccgcggtg atctggacca tgtggtgggc atggtgcatg cccgcgatct
     7921 gttccgtatc atgttccgca gaaaggctct gactcctgaa tacattcatg ccatacccaa
     7981 ggttcccaaa acggtgaagt ttgacaatgt ggtggaaatc atgaggaagg acaatgtgcg
     8041 ccttgccatt attctggatg agcacggggg gacttccgga cttttgaccc tgacggatgt
     8101 tttttccgaa gtaatgggct gggaccgagg ccgcattaaa gtgctgacgg agttgcctag
     8161 ggatgtggtg ggcttctcct gtgacgtatc cggtctggca cgcattgagg aattggggga
     8221 ggccatggac atggatttgg aggacgggga aatagacacc gtagggggcc tgattcttaa
     8281 tttgctggaa gctcctgcgg agaaaggaga tactgtcggc tatagagggc tggaattcaa
     8341 ggtgacccgg acggagaatg gcggtgtgga gcgctgtacc gtgacccgga ttactaccct
     8401 gatgcgggaa gaggtcctgg aagaggagga ataatctgcc atttacggac tgggcatgtc
     8461 aaggactgtg ctgcctgatg taaaagttcc ttggtggagc gcgtatgata tggcagaata
     8521 tttccgccat gtccgcccct gaatctgctg atgcccctgt gaaccgccct tccatgaaga
     8581 aatgggtggt tctcctcgtt gttcttgggg ctttgatcgg actgggcaca atggtgaagg
     8641 cctggatgga caaacgttct ggagcggcag cgcagccggt tgttttttca ggaggcagtg
     8701 agcactggga ccagaatgag gtgcctattt ccatgcagtg cggagcatgt catgaaaagg
     8761 aattccgtca gtgggccggt tcagaccatg cctgggcatt ccgcaaactg ggagatcaat
     8821 gggaatctga agcgttccat aacatgaagc tggacgctca tggcagcatt ctccaattct
     8881 ctacgaatgg tcacgggcgc atggtccatg acggtcagtc ttcaacctcc tggcgcgcgg
     8941 aatgggccac agggagaatt cccctggtgc agtatctggt tcctgccaag gacggcggtt
     9001 ttcatacctt gagcgctgca tgggacgtga accgcaaaga atggtttgat attttcggca
     9061 aggagtcccg ccagccggga gactgggggc actggacagg acgaggcatg aattggaaca
     9121 cgcataagaa ttatgatcct gttaaagatc ggtatgcttc tacctggacg gaacccggag
     9181 tgacttgcat tcaatgccac ggccccctgc tggacaagcc ggaggcgggg acgggttgca
     9241 tgatttccac caggaacaag ctaacgccac agcagattca tgataactgc gcttcctgcc
     9301 atgcccggag ggatgaattt gatcatgatt ttgccgtagg tgaccgcttt gacaatcatt
     9361 tccaactggt gcttcccgtg cagcccggcg ttttctggcc caatggcatg caaagggatg
     9421 aggattattg cgagactggt ctgaggttaa gccgtatggg caaggcggga gtgacctgtt
     9481 tggactgtca tgatccgcat acgggaacac tgaagcttcc tcaagaagat aatacgctct
     9541 gcttgaggtg tcacggtact ggagaagcgg taaacggcgt aaaagctccc gttattgaca
     9601 tggctacgca tacgccatgt cctcagtcca gcatgggtgc ccggtgtgtg gaatgccata
     9661 tgccggaaag cctgtatatg gctcgggacc cccgcaggga tcattctttc aattctcctg
     9721 atcctttgct gagcgtagag ttggggattc cgaatgcctg taccatgtgc cacgaggaaa
     9781 aaagcaatga atgggctgcg gaggcggtaa agaaatatta cggagctaaa cccaaaatgg
     9841 cgcagtacag ggaacggacc agggccgttc agcatgccta cgaaggaaag gaagatgttt
     9901 tgcctctgct tatggaatgt tttaagaggg aggaagtggg tgcatggcgc gctacgttat
     9961 tggatttgat ggatgcatgg gctcatgaac ccgccgtcca ggaagtggcc gcggcggcgg
    10021 ttaaggaccc ggatcctctg acgcgagccg ccgccgccaa agtgatgggg cgagtgggta
    10081 atcctgctgc aggcaaactt cttaatgatc cgttcagggt cgtgcgcttg caggcggaat
    10141 gggctctccg ggattccctg cctccggaca gtccgggaat gaaggagctt actgctgccg
    10201 ctttgcacca ggcggaccag ccttccggag ctatgaagct ggcccagata gccattagcc
    10261 gtaaggatgt gaaaactgcg gaattgtggt tttccaaagc tttgaaatgg gatgccactt
    10321 catccgtggt gcaccgtgat tatgccgttt tcctggcttc tcaaggccgc agccgggagg
    10381 cggtggatca gatgcaagag gctgtacgcc tggctcccag ggatgccaat ctgtggtatc
    10441 tcctgggact gggccagatt gaaaataatg atgagtcggg agctttggaa tcctttaacg
    10501 aagctttaaa aatagagcct tcctttattc gtgctttgtt taaccgtgct ctgttgaatg
    10561 aaaaagtggg ccggctggag caggctttgc aggatttgga aaactgttct tccctggaaa
    10621 agggaaatgc ggatattcct ttcactcttg cggtgatgct ttaccgcaat gggcggtacc
    10681 gtgaggccgc tgccgctgcc gcggaagccc tgcgccgcga cccgggacat gcccgggcca
    10741 ggcaaatttt ggaatcagct tctcgacatg gaagataaga aatagtatat tagcattatg
    10801 tgtatgaaat tttcatcttg gtgtatctta ttggcatcca cattcatgat ggggtcctct
    10861 ctctgttggg cggattcttc cggagtagcg gatgatcctt ccctccgcac ctggactaat
    10921 aaaaatacgg gcagtacggt ggaagcccgt ccagtagccc tgaatatgaa gaccgtcaaa
    10981 ttggtgacga cggctaaaaa gcccattacg atgccgctgg aaaagctttc cgatgaagat
    11041 cgcgcatggc tggaggaaca caaggaagtt atcggcaagc ctattgcaga atggtcgtcc
    11101 atgccgtccg gccctgtggc ggaggagatg aagggaaaga cttacatgct tgaaaacggc
    11161 aaacttaaaa agagggacgg gaaattgaat cccaagcatt tcattttgta tttcagtgca
    11221 agctggtgcg gtccttgctg ccggaatgcg cctcacagcg tggaggccta taacagggtg
    11281 gtaaaggata atccggatgt ggaagtaatc atgtgcaatc tggaccagaa cctggatgcc
    11341 gcccaaaaat gggctgctgc caataacatg ccatggccta tcctgttgaa agaggatttg
    11401 acggagcttg ccaagaaggt ggctccacgc ggtatcccta ccatgattct ggtggacaag
    11461 gacggcaagc ctattcagtc ctcccaaaac atggaacagt tggtaaaagc catcgactca
    11521 agccgttctt cccgctaggc ggcgcattgt ctgattgttt aggagatgtc ttctacccgc
    11581 aaatcccagt ccgaccgttc tgcggaaacc agcggcaaaa tcctccgtgc tgcacagaag
    11641 ctttttgccc tcaggggatt taatggcgtg accatgaggg cggtcgcttc tgaagcagga
    11701 gttaatctgg cttctatcgt ttactatttt gagaataagg agggactgta tctggctgtt
    11761 tacagacaat atgcggaacc tctgatgaaa gcgcgcatga agatgctgaa agaggcggat
    11821 cttcatccgt ccctgagggc gtatgcccgt gcatttatag aacctgcttt ccggctattt
    11881 ctggatgaga gcctgggagg gccggatcat gtccgtttat tgtggcgttt gccgcaggag
    11941 ccggaatatc ttggaagaaa aatctatgat gaattttatg ctccatctat ccaggagatg
    12001 atagcaagag ttcggagatt ctgtccgtgg gatgatgagc tttccttatc ttggcatgcg
    12061 catatcatga gttccatctt ccatgctacg ttggggaaat atgtcgcgtg catggatctt
    12121 caagaaaagg aaccgtggat gaaggatcag gagcacattt tgcagatgat tgtgaatacc
    12181 gccgtttttc tgatctcccg tccggctctg tctccggagg aagaggaaag gcagggataa
    12241 catggatggg ggtgcgcgtt tttcgttacg cttctcccag ttcttttctg agagttctga
    12301 ctgttcccgt gatgtcgggg tgtgtaagca gccaggaaac gatgactacc ctatttgccc
    12361 ccgcctccag tacggagggc agtgtgttcc cattgatgcc gccgatacag aagacaggga
    12421 aattttccgg tagctgttgc tgtacgcttg cgatgtcttc caggccaatg gcctgtcttc
    12481 ccggtttggt acccgtgggg aacaagggcc caaacccgat atagtccgcc tgctctccac
    12541 atgcacccag ggcctgttca ggactatgcg tggaccgtcc tataacggca tcctttccca
    12601 gcagccccct tacggatgcc aagtctccgt catcctgtcc cagatggacg ccgtctgcgc
    12661 cgatgttcag ggcaatttca ggataatcgt tgatgatgaa aagacagccg tatttacggc
    12721 acaggggggc aagctggcgc cccatatttt caatgtgttc cgggttatgg ttttttgccc
    12781 ttagctggag aatacgcagg ccgcctgcca atagtttttc tgttacaggg agtagttgat
    12841 gttcagctgt atatcccata tctacaatac cgtacagacg gcaggatgag aggcattcct
    12901 tcctgttcat ggcaagggat tgtaccggat aaaatcatcc cggcaagacg cttcccattc
    12961 ttgaccctaa aagagggtgt gatacactcc ccgcattgtg tcacccgcct tatattccgc
    13021 cgctaaaagt gtattattcc aaatgaatcc ggaaactgcc cacaaggtga ccttgtgggg
    13081 gttgcgcctg gcagaaaaga tgcgtgtact gcctctagta atgggggaag ttccttctga
    13141 tcctgtggaa atcctgggga tgaaattccc caaccgggtg ggacttgccg ccggaatgga
    13201 caaggaagcg gatacggtaa gcgcttttgg ccaggccgga ttcggttttg tggaagtggg
    13261 tacgctgacg ccccggccgc aaccgggcaa tgaaaagccc cgcctgttcc ggctgattcc
    13321 ccagaaagct attatcaacc ggatgggatt taacaatgaa ggtattgccg ccggagtgga
    13381 aaacattcgt tccgccactc gttttcacgg tgttctgggt gtcaatattg ggaaaaataa
    13441 aataactccc aatgaggatg ccgcccagga ttaccttact tgtctgcgtg cggcctggcc
    13501 tgtggcggat tatatagcca tcaatttctc ttctcccaat acgccaggcc tgcgcgacct
    13561 tcaggcggcg gaacccgctg cccgtctgct ggcttccctg aaatcggagc aatccaacct
    13621 ggctgcggaa acgggtcgcc atgtgcccat ttttatgaaa gtggctcctg atgtgacaga
    13681 tgagcatatt gcggagctca gccgcgtttt tctggatgag ggactggatg gtcttattgc
    13741 tacgaatacg actctttccc gcgtcggggt agaggccaat cctcggcatg aagaagcagg
    13801 cggcctgtcc ggcgctcctc tgacggaacg gtccacggaa gttattggag cttttgcttc
    13861 cgaattgaaa ggacgtattc cgatcattgg cgttggaggc attatgaacg gggcggatgc
    13921 cgtcgccaaa atcaaggctg gagccagcct ggtgcagctt tataccggct tcgtttatcg
    13981 cggcccggac cttatccggg aatgcgtgga agccatgaag gccgaatgcc ctgttcaccg
    14041 ctgatttcct ttattaaccg cttacaggat ccatccatgc ccggttcctt cgtacacctt
    14101 cacaatcaca cggaatattc gttgctggac ggcgccgtcc atatcaagga cctcattgct
    14161 gagtgttccc gcatggggat gcccgccgtt gctgttacgg atcacggcaa tttgttcgga
    14221 gccattgaac tggtcatgga gacaaacagc ctcaataaat ccgtggccgc ctggaacaag
    14281 gagcatccgg aaggaccgca gaagcaggag gtaaagccta tctttggttg cgaggtttac
    14341 ctttccccga cgcccatcag cgtgaaaaaa cagcttcccg gccgccgcaa atacacacat
    14401 ctccttttgc tggcggaaaa tgaaaccggg tggcagaatc tggttaaact ggtgtcccgt
    14461 tcccacttgg aaggctttta ttataaaccc cgcgtggata tggaaaccct gagggaattt
    14521 catgagggcc tcatctgttg tacagcatgc attgccggac cgcttaatga gtggctgctc
    14581 aatgaccagc ctgaaaaagc agaggaagcc cttctggcgc taaaggatat ttttggggac
    14641 gacaatattt ttgtagagct tcaggaccat ggcatggagg aacagaaaaa atgcctgccg
    14701 gagctggtgc gtatcgcccg caagaccaat acccccattg ttgccaccaa tgacgtgcac
    14761 ttcctcagga aggaagatca cgacatgcac gatgttctga tttgcatcgg aaccaacgaa
    14821 aaactggctt ccccaaaacg catgcgctat tccacggaag tgtatttgaa atctccagag
    14881 gaaatgcggg aggtgttcaa ggattatccg gaggccgtgg aaaatacgtt gaaaattgcg
    14941 gaacggtgca atgttaccat caagctggat tccaccagta cggagaaata ccccgaattc
    15001 ggcacgccgg acgggtcgtc gcgcgaagaa tacctgcgta aagtatgcta taaggggctg
    15061 gaagaacggt acggaaagga acgtgtagct gcagataagg aattgcgcga tcgtctggat
    15121 tatgaacttg gaattatcaa ccagttgaaa tttccctcct atttcctgat tacggcggat
    15181 ttcatcaact gggccaagga tcatgacatt cccgtaggtc ccggtcgcgg ttctgcagcc
    15241 ggttccttgg tggcttatgc catgaaaatc acgaacatag atcctctgag attcggatta
    15301 atctttgaac gtttcctaaa tccggaacgc gtcagccctc cggatattga tattgacttt
    15361 tgccagacac gccggccgga agtaattgat tatgttcggc agaaatacgg agagcgcgct
    15421 gtttcccaca ttattacgta cggcaccatg ggggccaagt ccgtactcag ggatgtagcc
    15481 cgtgtgatgg atatgtccta tgcggacggg gaccgtattt ccaaacttat cgaaaccggc
    15541 cccaaggtga ctcttcagtc cagctacaag agtaacgagg agctgaggga gttgattgcc
    15601 tctgatgagg ggtatgcgga attgtggggg tacgcgaccc gcctggaagg cttgatccgc
    15661 aacgtggggg tacatgcggc aggtgttgtg atcggtgacc gtcccctgga cgagcatgtg
    15721 gcccttaccc gtgacgatct gtccgatcct catgccgctg tcgtcgccca gtgcgacatg
    15781 agcgcgattt atgaagtggg gcttttgaag atggacttcc tgggtttgaa aaccctgacc
    15841 gtcatgcatg atgcggagca atacgcccgc tggcgcgtgc cggagttcaa actggattcc
    15901 gtccctctgg atgaccggga aaccctggac ttgctgaaca ggggggatac gatgggggtt
    15961 ttccaattgg aatccggcgg tatggtggac acttgccgcc gttacgggat tcagaaaatt
    16021 gaagacatca ttgacttgct ggctctttac cgaccgggag ccatgcagtt tatggatgaa
    16081 atgattgaag tgaagaaggg actgcgccag gtggagtatg agcatccttt gctggaacag
    16141 gtcagtgggg aaacctacgg cgtgatgatc taccaggaac aggtgcaggc ggcggccaag
    16201 ctgctggccg gctatacgct tggcggggcc gaccttctgc ggcgcgccat gggcaagaaa
    16261 gacccggcga agatggccaa ggaaaaggcc cactttgtgg aaggatgctg gaaaacaaac
    16321 cagattgatg aaaaaacggc tacagctatc tttgacaaga ttgaaaaatt cgccgggtat
    16381 ggttttaata aatcccactc cgcctgctac gggcatattt cctactggac ggcctatctc
    16441 aaggcccact tccctgtgga attcctctgc gggctgatgt ctaatgaggc gaacaatgac
    16501 aaaatcggag tgttcattca ggaagccaat cgcatgggta ttgagatcct gcctcccgat
    16561 gtgaataaat ccatgctcaa gttcactcct gagaaaatgc cctccggcaa gctggccgtg
    16621 cgttatgggc tggcggccat taagaacgtg ggagaggggg ctatgcagat tcttctggaa
    16681 gaaagagaga agaatgggga attttccagt atggaggaca tttgtaacag gctggattcc
    16741 aaatctgtca acaaaaagat tctggaatcc ctgattcgtg cgggagcgct ggactggacg
    16801 ctggagccgc gttgtgttct gttcgaacgt gtggacctgg ccctcagcgg agcttcccag
    16861 gtacataagg ataaggctct ggggcagggg tctctgtttg acatgacctt tgaagcgccc
    16921 agagtgaagg atgaacctgc cgttccagag tggtccaagg agcagcgtat gggagatgaa
    16981 aaggatctgt tgggcgctta tttttgcggc catcccctgg actccatgcg cggtgtcatt
    17041 gatgcggaaa aatatacccg cataggactc attgacacgc tggaaccaca cgaactcaag
    17101 cagaagcacc agattgccgg catggttcgt tccgtcaccc ccaaaatcag caaggccggt
    17161 agaaaattcg ccattctgac gttggaagat tttaccggca gcatagaagt gttgttgtgg
    17221 tcggatgttt atgaaaaggc tcaggaaatg gagggaggcc tggagccggg cgttttcgtc
    17281 tccgtgcgag ccaacatccg ggaggatgac cgtacatcct ccaaaagcgt ggctgcccag
    17341 agcattgacc cacttggagg caaaaagcgc aaatccaaat ccggggatca ggggcctctg
    17401 aatatcattg tcagtcccct ccgccacacg agaaaaaatc tggaacagat acgggatatt
    17461 ctaaaaaggt atcccggtcg ttccaaagtc aatctcacca tcaaggacag cctgggccat
    17521 agccaaattc tggaactgga tgaacggttt cgcgtgaatc gctgcccgga gctggaaaca
    17581 gagctttcca tgtacaacta aggccatgcc gggaggaaaa ctgtcattgc cggaatctga
    17641 acgcctggtg ttacgggagt ggcgggaaaa agactggcct ttttttgcgg ctatgaacgc
    17701 caatccccag gtgatgcgtt tttttcccgg tgtcatgacg gacaaggatt ctctttccat
    17761 gtggaaccgt atgcgggaag aactggatga aagagggtat ggattgtacg cagtggaatt
    17821 gaaacattcc ggttccttga tcggccttct cggctttcat tgggctgatt ttgaagcgga
    17881 ctttacaccg tgtgtggaaa taggctggag gcttgtgccg gaagcctggg ggtacggata
    17941 cgctacggaa ggcgcccgtg catgcctgaa acatggtttt gcacgattcg gctttgacag
    18001 ggtatattct tttactgctt gcgtgaattt cccttcacaa gctgtgatgc aacgtgttgg
    18061 aatgcgaaaa atagcggaat tcaatcatcc caaagtagct ccggattcca ttctttatcc
    18121 gcatgtactt tatgtgaagg ataccttccg gaaaattgaa cgggagtaat cttgaaggaa
    18181 tgccacctgt tgaccgggta aaaagtgcag ggagggggag aaggcacctc tgctgccagc
    18241 cgtgacttaa aagggtgtaa cttgctacgg cattaacccg gtataggttg agtatttctt
    18301 ccccctgtct gcaccagaag ttgctcatca ggaaaagcgg ggaagattcc acgatgttgg
    18361 ttttattatt gtgcagcagt ttttttgatg gaaattcggg ttaatccgta tttttccctt
    18421 tctgaaagtg cgcatgcacg cctcggcatc aggaaccgta aaagtccgtg acacagtttt
    18481 ttccaatgga tgttgtattg ttaagatagg ggggtaacac attatcccgt ctgggaattg
    18541 catccgaaaa cgtccgtttc tcttaactgt cataatatgg agatgtcata cataaagtta
    18601 tctggagata ttggcgagtt tttataaaga tatttgcaat taaataattt ttagtcatca
    18661 atgaatgatg aaataaatct cgactctgtg tttttccggg aaaattatac ctaggataat
    18721 gacaatggaa atatgaacgc ttttattcgc gggtgcttct atccttcatg cgggttgcct
    18781 ttcacggttg gcccggaaac gatgaaacaa tagataccgt catgtttgat ccaaatcaaa
    18841 aacccgatac agcccgggcg gaaaattcca ttttcggttc tccggaggca gataaccttc
    18901 tccccaagga tgagttcccg gattatgcga tgcgcgcgga ggacgctttc caattggtgt
    18961 ccgatgaatt gatgctggac ggcaatgccc gccagaatct tgcaaccttc tgccagacct
    19021 gggatgaaaa gcaggtcagg atgctgatga atctcagcat taacaagaac atgattgaca
    19081 aggatgaata tccccagaca gccgctattg agatgcgctg cgctgccatt attgccaatc
    19141 tttggaatgc cagccagaat gaaaagccta ttggttgttc cactatcggt tccagtgaag
    19201 catgcatgct tggaggcatg gccgccctgt ggcgctggag agcccgccgg aaagccgccg
    19261 gcaaacccat tgacaagccc aacctggtat gcggccctgt ccaaatctgc tggcacaagt
    19321 tctgccgcta ttgggatatt gaaatgcgtg aaatcccgat ggcgcccgga aaatggaaga
    19381 tggatgttga ccggatgctg gaacagattg atgagaacac catcgttgtg gttcccacgt
    19441 tcggagtgac ctatacgggt gcttatgaat tgccggcgga gattgccaag gccctagatg
    19501 attatgaaaa caagaccggc atcagcgttg acatccatgt agatggagcc agcggcggtt
    19561 tccttgctcc gttctgcgcc ccggatcttg tctttgactt ccgcattccc cgcgtcaagt
    19621 ccatcagtgc ttccggccat aaatacggat tggctccgct cggagtgggc tgggtcgttt
    19681 ggagagatat ttctgaactg cctgaagggt tgatcttcaa tgtcaactan nnnnnnnnnc
    19741 aactatcttg gcggggaaat tcccaatttt gccattaact tctcacgacc ggccggacag
    19801 attatctgcc agtattatga tttcatccga ctgggcaagg aaggctatac gaacatccat
    19861 acgcaggcct acgaggtagc gaagtacatt tcagatgagc tttccaaact gggaccgtac
    19921 gaattcattt gtacgggcga tccggaaaaa ggtattcccg ccgtctgctt cttcatcaag
    19981 gatggagccc gtgcgaacta cacgctcttt gacctttcgg acaagctgag aacccgcggt
    20041 tggcaggtgc ccgccttcac gctccctgcc aactgtcagg atacggtggt gatgcgcgtc
    20101 atggttcgcc agggcttctc caaagatttg gcagacctgt tcctggaaga ctacaagcgc
    20161 atgctctctt tcttcgagaa acacccggtc tcttctccga tgacggcgga agaaggaacg
    20221 gctttccacc attcctgatg gaagaagctc tgctgagcga tttttgttgg atgaataagt
    20281 gtgtgtaagc gcagctccgc agttgtcaag gtttgtgcgt ggacctgacg gctgcggagc
    20341 tttaataagt gtttatttga tacgtttcga tatttcagca gggtattcaa cagccccagg
    20401 gaggtaatcc ggggcggaaa ggctgttctg ctatgggacg gatgcagaat aatatgttcc
    20461 ttttctttgt tgtggaacgg aacacctccc tttccagaaa taatccggag gggttggata
    20521 ggnnnnnnnn nnggtcaggg aggtttgttt ttcccgttta tctagaagta ataattggca
    20581 cctgggtatg aggaaggaac gtacggaacg gcataattct atctgtcgtg aatttggccg
    20641 catcttttag ggaggcggcg tttcataccc ccggcttggg gctcaacaga aacctgcaga
    20701 ccgctattat catcgcccgg ataggccatc tagggagaaa accgggggct tacttccatc
    20761 ttttttgtct tcgctttctt cttttccgcc tgtcggactg gttcctccct cctttccttt
    20821 ggtggcttta atatacagtt gaccgtcagt cccgctttat gaagcaatac ctcattcatg
    20881 gtcggaaggg tgctgcgtga ctatgccgga actggttgct ggaatcaggt ccgggagaat
    20941 tgttcgtgtt ggtgaggaca acactttctt tttctttggc catcatcggg aagccctcct
    21001 ccgggaacgt ccatattgcc tcctattgcc gcttcggaag agtagatggc ttctccttcg
    21061 gatcttgagc t
//
LOCUS       DAWEMX010000032        20555 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1084_length_20555_cov_10.654098, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000032 DAWEMX010000000
VERSION     DAWEMX010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 20555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20555
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_20555_cov_10.654098"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3428..3437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3860..3869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4620 a         4997 c         5779 g         5139 t
ORIGIN      
        1 tatgttgaaa atgaacgaca gagccgataa agtggccatt gacggaatca tccttgctaa
       61 ttctcaaaat atcctgaagt agttgctggc attgtgcaat ggaccagcag taggttcttt
      121 ggtaaatagg gataacaaac tgtctggggc cttttaaaag cgacaagaga ttttcggcat
      181 tggccttcat ggagagcagt cttgcataaa aatgaatctt gctcaagaat actcgttgaa
      241 tgaaaattgg aaactttctg tctttttacc tcgttaattt gaaagattca ttattttgaa
      301 ccggagaacc caaatccgtc ttatctgaaa ttttcggatt gcggggagtg ccgtctggat
      361 aagaggtttt gtaaatgccg aattcctgtg cctttttctc cagagcgcgg gtgacgttgc
      421 cggaagcggc gttgatgcgg aggcggcttt cactgatgta ggaaggattg tggaggggct
      481 gggggaatat ccttggcagg aactgcgggg atgacggcgg gaggacatcg tcggaacagg
      541 aaccgggatg attccgcttc cagctccgga accatacggg agaagtcagg tgttggtatt
      601 gttggacaag accttttccc ttgatggcat acacgcgttc cgccgccagc agggtcatgt
      661 tatcaagctc ttgctgcctg cgccatgcct gcctgatttg ttccagttct tcagcgttcc
      721 tggcggcgtt gatggtttcc agcgtcttct ggacggaagc ttcctttttg ttggccagat
      781 tgaggaacat gcggctatag taattatgaa attcccggga ctcccggttg aatgccgcga
      841 tttccggctc ctgaagcagt tcttcgacaa gaagatcttc aggatttttc ctggtctctt
      901 gcgtcaaaac gcctgtctta taaagccccc aggtaagcgc tccccctgta acgagcatca
      961 cggcggctat cgtcataatt ctgctccgga gtcttcgcct ccgatcgctg cgaagggcat
     1021 gatccatccc ggtcagccac tcttctggag aaagagagcg ttcctccctc ttccgggaga
     1081 ggttttgaac aagaagacag cttacgcatc tcagggcttt ttctttttgg gccaatgatg
     1141 ccaataactc ccccgcatgc gcttcgccta cctttccgga catggtgttc cagggatagg
     1201 aggtcatcag ttcgtaaaag cacgctgcca gagagtattg atccgcccgg atgtcgggag
     1261 aggtgttttc tgtgaacaat tccggagccg agtatgcgga atgagccagg atattgactg
     1321 tgccatgagg agggcgcaga gaagcggtgc cgaaatcagc caaaacgggc tggccttccc
     1381 cggtaaagag gatattgccc ggcttgatat ccagatggac gatgtgatgt tgattcagac
     1441 aatcgagggc cgaaaccaga gtctgaagcc atgagatgat ggtgcgtgga ggaatgcgtt
     1501 tgcctgagtg gatgacgtcc cgcagggttc cgccctcaag aagaggcatc acataaaaac
     1561 aggtggtggg cgaaaggctg aatacccgat gaatcctgat gatggcggga tgttccagag
     1621 aagcgatcag ccgggcttcc ctgatgaact ggttgacgga ttcggcaaag agggattccg
     1681 tgtcttcatc cagcggaatc aggcaatggg agtcggcatc tctggcgcaa agtccctggg
     1741 gataatgttc cttgaggacg acctttctgt ccagcgtcct gtcatgagcc agatatgcga
     1801 taccgttcgc tccgtggccg aggacgcact caatggtgta aggaccaact ttttgaccgg
     1861 gaggaaggaa agcgggggct tcatgcatgg cggatgaata attcaatcat gccccggcag
     1921 tacggagaaa atcaatccct ctcttccgaa aattcctcgc gttcgatttc atcaaccagg
     1981 gaaggagagg cgggagttcc ttcgcgaacg atttcattga tttttgccgc cagaaaatcg
     2041 gtcaacgtca ttttactatc gagagccttg cgctccagag ccgcttggac atccggcggc
     2101 agttgaagga tcacgcggga ctccgtttcc accttcacgg acggaagagt cacgggcatt
     2161 tccgtcatca gggaacggat gatgtgttcc ttgacttcag gaatcttttt cttggccatc
     2221 caattgtaga cagtcacttc actgacaaaa cactgctggg ccagccattt ataatccaac
     2281 ccacgggcct tgagccaacg cttgacgtcg gctttcaact ccatctgcgg tttggaatct
     2341 gaactcatat tcgtgatact agcttttccg ctattgcgcg tcaattccca agaggcgaat
     2401 gccggaaaaa ccatcgtcgg aagaaggtgg ataagtgata acaaggtttc ctgaacaata
     2461 tctattcttc cataatagct ataatactat ttgacaaaat ggggtggata gatataaaaa
     2521 atatctgtat ggcgaatatg caatgcgata tcaccgagct aatcgagaag ctaagcgatg
     2581 aagccagatt gtatcttctg agacgaatgg cctcccacaa tctttccttc cggcaagttc
     2641 tcctggagct tgtcatcggg ataagcagag aggttgcagc cggctcgaaa gaacaagaaa
     2701 ccgcatgttc cgcctgtttc attcctcatt taacctctca ctcaaaagtt ctccaccaat
     2761 gaaaacattt tgcgtctccc tcatggcttt ggtagccgct cccctctatg tcctggcagc
     2821 ccatgcaagt ctgtccactc cgaatactca cccgacggca catgaaggca tgaaatgcca
     2881 gcaaagcaat ggagtcaaag tgtgccccgc ctgcaatgga agcggcaaat acggaaacac
     2941 cacctgcgtc aactgtggga gtaagggcat tgtccgtaca aacggacaga ggctgtaagt
     3001 atttttatgc atagatcaaa aagtgaaaat taaaaacgta ttatttatat gtattgccct
     3061 cttaggttct ggactattat ctaattgctc tcctacaaaa ataggaggcg gaggcaagaa
     3121 gcaaccttat gatcgggatt caggacaata taattccgta atattttttt tgcgacgatt
     3181 ataaaattaa atatattatt ttaattatat ttatttcttc gctgttttat gtgcgaatta
     3241 tttgcgatag aaaaagcaag taaaataatt tatattaata tgtataaagg atggaatata
     3301 ttttctgcta ccgccaatgg gagatgcgat attataaaaa aactcattaa gcaaggatgt
     3361 gatgttaatt caagggattc aaaaggtaat cctgtgcttt atcatgctat tattcataat
     3421 caaaatgnnn nnnnnnnttg atttattgcg aaaatatgga gtccctaaaa gttctagaga
     3481 ttttgaattg ctcataaata ctgcgttgtc atatcgtaat ttaaattttc tagaatcctt
     3541 gatatcagac gtaaatatac ctttacttcc gctggattgc tttgggaggc gataccgtgt
     3601 atacgatatt tttagaattt ctttgtatga ttcgttttac tgtgatgtat gtaatataat
     3661 attgaaatat actacttcta tgaagaaaga aagatctttt atgatttttt tattaaaaga
     3721 actatatgaa cgctatagat attctggaaa atattcaaaa ttaatactat ggggatatgt
     3781 taaaacatca gtagaaaaag atggggatgc gaatgttttt tatcgtaaat tctttataag
     3841 aaggacattt ataagatgtn nnnnnnnnna aaaacagcta atataacaat tattttttgc
     3901 tatgattgag tatgttttct tttatgttgt cggattatca gttttttcag cctttctctg
     3961 tgaaggatcc ggtatcgtcg cctggaagga gcaagctttc catgaggact ccatggccaa
     4021 aatcgcaaca tacagcagga gaaagctaag tggaaacaaa gggatattca acgtgaaggg
     4081 aaaagatatc tatctgcctg agaatctcta ttggtacatt gatattccca atggccttcc
     4141 tttggaattc tccgattcgt ttcaatatga gatgctcctc aagcagaaat atgagatggg
     4201 cgtctttcgg aaacgcttcc cgaagtcttc agccatcctc gatccatggc tgaagcgatt
     4261 gcaggaatat caggacaact atgattccgg aaagcgtttt gtcaacggcg tctggatgga
     4321 aaaatcagcc tatgagcaac agcaggccgc acagagggag aagaggaacc aacgccgctt
     4381 gcaagaagaa cagaagagaa aagaggcaga taaaaatact gttgggacgg ggtccaggtc
     4441 tgccgggaga gaagaaaaat cccgaatatc gtacgcggac ggttccttga aggaaatagg
     4501 cgacgggagt gtcgaggggg ccaggacagt cctcaaacga ttggagcaga gtaaaaagtc
     4561 cctggatccc gcagggtgga atcaggcgga gcagctcatt caatccatca aacggctttt
     4621 taccgcgcag tccgcttaca aaaaggcctt gcagcagcaa gccggagcca aggccaaagc
     4681 ggaacaatat gaacgccagg cggagaataa catgaggccg aatccattga cgggagagcc
     4741 taactcctat atggccaatc gtgcccgcaa gcaggcaaga gagattgttc tgaacgcgga
     4801 acatgttctt accagcggaa agagggaact tctccaggcg ttgaagcaaa gcgatacgct
     4861 ggcctgcacc ttgtataaga ccattcctca ggacgcggag agcctggctc ttgccgttgc
     4921 ggcgatagcc gagtcctatc atggagagat agatttcaga agccggtttg aggaagtcag
     4981 ggatgaagaa gacaggcgtc aggaagaact tgcccgtgaa caggaagaaa gggaacaaca
     5041 acttgcccgt gagaaggaag aagaaaggct ccgggaagaa agagagcaag aagagcggtg
     5101 tcttagggaa aagcgggaag aaatcgctcg tcaaagagaa gaacgaactc aacagattgc
     5161 acggcgtagt caagaggaag aacgaaaagc cgcagaagag aacatggatg cggtaaaagc
     5221 gcctttcaca ccatctcatc caacagttcc agacaggccc gaggcacctg cagccccacc
     5281 catcaagtac atgagtgcta ccgacgccaa caggatggtg tatcgcactt tgggctctct
     5341 cgacccgcgt gccttggagg gaattgatga gaagtacagg aatatcattc aaacatccac
     5401 ggaccccgtt gaaatcgtaa gggctgccga agaggcttat gccgacaagg gctacatcga
     5461 cccggccatg cgtcaatacc tgatcaacat ggcccattac cgtgctcttt cgaataaaac
     5521 tgggcaggga acagcctacc accccagtct ggccagatcc tttccctcgt taccttctgt
     5581 catggaaatg ggagattatt catgggagga agaggttgac aaaatgatag ctgcttcaaa
     5641 ggatacctat aacccctatt tgtctctggc atgcaggcaa cgagccaggg acattatcga
     5701 agcgagaagt cgtattatgg gtggttccat ggatcgcccg gaagatattt atcggagacg
     5761 tggcatttca aaagatacag atcctgtttc agaggacacg aattccagct cactttccgg
     5821 gatggctctc ccgttgattt ttctggggct cctgattgtc tttcctttag gaattttttg
     5881 caggttgcgc aaaaaattgt aacggcatca cggatatcag aactcaattt tcaaatcaaa
     5941 gatgaaaaaa tattttattt gcgacgattc cgggaatctg caaggcccct attccgagca
     6001 tgtgcttcaa gttctccatg aagagggccc catacggtcc gatacccgta ttcgtgaaac
     6061 tcagtcttct gaatggggca catattcaga cattgcggta aaagttttag agggcaatcc
     6121 ccggaaggag gctgatgaat cctcttatct gattgcgctg ccggatggag aaactgaagg
     6181 cctttacacc aaagaacaaa tgaaagccct gtatcgcaac ggccgcttgg ccggatccct
     6241 tctttgctgc aagacgggcg aatcctcatg ggaagcgctt gaaacgttaa tcggaaaaaa
     6301 ttttcaacag gctgcttctc ctgaaatcac aggggaaagg tgggaaggat cccgttcttc
     6361 ttgctcaggc gaagatgacg agaattctca tttaatgccg gaaatgcctc ctatgcccaa
     6421 gacatcgcct ccattagaag aaaacgcttc atacagcaat cctccattgc aggatgaaag
     6481 aagtactccc tctgggcagg ggtataacat gaaagcgttc tgggcaatga ccatgctgta
     6541 tcttttgtcc ccgttgctga ttatagtggc atcagcaacc tatgcaggtg atgcccggtt
     6601 taccctaacg gttataggta taggaatatt attctttttg tatgcacaaa tttcgttgct
     6661 gatcaataca tggagggcgt ggaaatgcat acagcccatt gcccggtatg gtcgtcgcgt
     6721 cccctcagca gggcaggctg tggggctgtt gttcgttccc gtatttaatt tatactggat
     6781 gtatgtctgc tttgtcggat tagccgacaa cgcctcctgg tttgcccgaa ccacccggca
     6841 aaccagcctc cgactgaaag catggccgat gtttattttt gtcctactca cacagcttgc
     6901 tttcgggagc agtataatag caaacggcgt tttacctgaa tcccgccccc tttttaatct
     6961 tctttccttg ctttggttcc catggccgat cgcggtgttc tggtacacgg aaagaattat
     7021 tcagtcattt ccacgtcctc aagaaaggaa aaagaaatat tcacaacaag aagaagcaag
     7081 atgaagtctt atttgatcaa actgccggac ggaactcaaa tggggccgtt cccatgggag
     7141 aagatagata aactgtatgc ttcccgcaga atcagcgggg atgatctggt atggacggaa
     7201 ggaatgccgg catggcttcc tcttcgtgaa gttcaaaaac agaaagacga accgcattca
     7261 caacaggcgg ctacatcaga cgcttccaga ccggggaagg aggaagcgtc ttattttctt
     7321 tcatcgggcg gagaaagccg caagggaccc tatacattca aggaaataag caactggtac
     7381 gcaaccgggc agctttctcc tgaggtgttc ctgtggaaag agggaatggc cgagtgggta
     7441 agagtggggg aatttatcag cggctgtcag gaggaaagct ccttctgggt gggggttttt
     7501 ggaatactat catctctgac cggcctttct tccgtagaaa gattcagttt gaaaagcata
     7561 ttcaaagggg tattccgtcg caggacggag caggaaagga tagatttttt tacgtcaggc
     7621 gggtcgaaat ctaccccatc actggaagaa atatctacgg attggccttc gccatggctc
     7681 ttcactcgcg ttctcgcgct ttccctattg atgtgcatcg ggtgggcagg catgcttgag
     7741 gcatttaata acagtcctgt agtttttggt gctgctagtt tagggctgtt gttttccata
     7801 agttttgctg tgcccttttc cattcttgtc ctgtttgcgg aatttaatgt cagaaggaac
     7861 atctcatggt attcgattat cgtcctggtg ttaggtggag ggattttgtc tttgattgtt
     7921 acaattattt tgaacgagct ttccggtaat cccagggctg cttactgggc cggtcccatt
     7981 gaagaagccg caaaacttgt tgttgccatt ttctttgccc gaaaattctt tctgaatggg
     8041 caaattttaa acggcatgtt gtgtggagcc gctgtaggcg ccggtttcgc tggttttgaa
     8101 acagcaggtt atattattat ggttgctgaa catggtctcg atccattaga cacggcagta
     8161 ctgagaggaa ggcttgctcc ctttgctcat gtttcctggt cggcaatcat ggccggggca
     8221 ttctggcttg cgatgaagaa acaacggcag aacaacattc cctccattct tcaacccccc
     8281 ttttggcgaa ttgccgttat cccgataggg cttcacatgt tttggaacag tcctttgttg
     8341 gataagtctg aattattgcg ttatgcaaaa tgggggatat gtgccgtcat ctgctggagt
     8401 atggtctttt tactcctcaa tgaaggatta atccaagtca gacaagccag ggagcataaa
     8461 atagaccttt agtatcatgt accgatatac tgcgtcctca ttatgcgcca gcgctgtttt
     8521 tgccaacagg cagggggatg ctgtatttca atagcaaggg agtgtctaaa atacggtgat
     8581 tcagcaacta tcatcatcca tctttcaagt tctttcccca aagtgggcaa tgactggtca
     8641 agctcatcat tcatgggcag gggctgtctc tgataaccct ttttacgcca tgccatatgt
     8701 tctgccaggg gaacgcattt caccaacgag gaaaacaaca atgaaaaaca gaccatccat
     8761 tactaaagag gaggatcata aaaacaagga ggtgccggac atttgtctcg gaagaactgc
     8821 cgcacactat catgatgaac ttatcaggtg cattgagcag gagtgcggcc cctcaattcc
     8881 ccatgttcat gataagcccg tcctttcgag aaggagcaag gcgcttgcct tctttctttt
     8941 gttcatcctc ttaatgaccg gcgccattgt ctggcattgc tttgcaaacc gggcagatcg
     9001 ggatatctat gaatatcagc atgttcctga aaatgatctt atacggtcac agatagcaat
     9061 tttatagatc cgtaagaaaa acaggaataa tgaatactag cccggcaatg actgaagagg
     9121 tggatctcct atcatgaaag acaacaccat tcaataattc tgggcgtata gcgccaatgt
     9181 aaccggccct gaatacctga aaggggggcg tgcgcttgaa gaagcttgaa atgtcttgag
     9241 aatgcgtgct ttttgcggag gaatggaagg agggatgaag aagtagcatc cgtaaatgcc
     9301 cgtaaggggc attccgccca ttaaggacat ttccgccagc gctccgtaaa gtatgctcgg
     9361 agaactggct ctccattcac ggactggccg tggttataaa aattgttttc gtgtcttgtt
     9421 gtttcaagaa agcaaaaacc gcaagagctg gaaaccagca acttgcggta tgaaagagga
     9481 tggtgctcgg gaggggactc gaacccctac ggtttatcac cactagatcc ttagtctagc
     9541 gcgtctacca attccgccac ctgagcatgg aggccttaag gcggggagtt tttagcgcag
     9601 agaaatgaaa atatcaagct ttttcttctt ttttatcttt ccgggcccgt ttttgcggac
     9661 aggtttttct taccggaccg ggagggaggt tgtcgagggc atgatagcag ggcgcagagt
     9721 aacggctccg gtatcatatt tttccgaggg tttgaagagc cagccggggg tggtgttggc
     9781 cacctcaaac ctggtcggtt tgaagatggg cagcctcaga ttggaattgg gttcgtaacc
     9841 gtatttgccc gtgtatttgc cgtataccct gtattcatag ttgttgtcgt aggcgtagcg
     9901 cggatttccg gtgtattccg gcaggcggtc cggggtgcgg caggcgcttt catccatgat
     9961 gacgagttgg gccgttctcc aagtctggcc tggctggcgc aggtatcccc agaagcgcgt
    10021 tgccggtaca tggtagcggc ggccgacgaa gtagtttccc ctgggttcgc gggctatttc
    10081 cgcattgcgg gcgttgatgc cggcaatgtc tttttccagg gtgttgcact gggtcaaggc
    10141 gggaaggagg gcggccccca gcaggggaag caggcgtttg ttcatgatga cgatggtgtt
    10201 tttttcttat ttaagagcat ttgaatggcg gcggaagccg caaagaagcc aaaggttccc
    10261 gtgatatggg tgacggagcc gaatccggcg tcacaaccca ttttcccctg gaattccgga
    10321 tcacgtgtgc aggaggttgt tccgtcgcag gtaggatata cgggcgtttc ctgggagaaa
    10381 acgcagggga ttttcagttt ccgggctttt tctccaagcg gaagggcata atcttttttc
    10441 agcctgtagc gtaagctgga gagcagggga tcccccctgg tgcgggagag gtcgtctatt
    10501 tctatccggg cgggattgat gcgccctccc gcgccaccgc aggttaccag cggttgcttg
    10561 ccgcggtaac aggaggcgat gaggtgggcc ttgggtatca gggagtcgat ggcgtcaatg
    10621 atgacgtccg gttccgccga cagcagtttt tccgcgttgg aggccgtgta gaaattgctg
    10681 atgggaatga cttccgcttc cgggttgatt tcccggatgc gtgtggccat ggtttccgtt
    10741 ttggattgcc cgattgtgga cgccaacgca tgtatttgcc ggttggtgtt ggtaatgcag
    10801 aggtcgtcca tatccatcag aatgatagtg cctacgcctg accgagccag ggcttccgcc
    10861 gcccaggaac ctacaccccc aatgccgacg acggctatgc gggcgcttct gagaagagat
    10921 agtccgctgt ttccgtacag gcggcctata ccgccgaatc tggcttcata gttcatgacc
    10981 acgtgtctgt tccagagtat gggagattaa ggagaataag ggcgcccggg tttctgtccg
    11041 ttgtttcatc ctgaaaatat ggagacgcgg gaaatccttg gattccggac gccacagcca
    11101 gcttcccgtt attccgggaa ggggaggcgg gacgcctgat ggatggcgtc cgttgaaaca
    11161 ggtttttctt ataatccgcg ttctggccgc gcttcacggg gcttccgcca tgtccggaac
    11221 atccgcagag caccgccacc gggcacggga agaggaatgc tgctgaagca tgcatgtcag
    11281 gaggccggtt tttccacgat tttgctgatg cgggcgatgc tgagcgccct gcctgtggct
    11341 tcatctattt gcacgacggc tccgcagagg cgcacgggcc agttcgctac ggggaatttg
    11401 gccggcatgg aggtgcggaa acgctggaga ataggttccc gttcccgtcc cagcaccccg
    11461 acggcggggc cgcacatgcc ggcgtccgtc aggtaggccg tgccgcgctc cagaatggtt
    11521 tcatccgccg tctgcacatg ggtatgtgtc ccgacgacgg cggaaaccag gccgtccaga
    11581 ttgtagccgg tggagatttt ttcactggtc gtttccgcgt gcatgtccac aaagataacc
    11641 cgcacgcctt tttcttccct gagtcttttg gcttccgctt ccgagatgag gaaggggttt
    11701 tccagcggcg gctgaatgaa tgagcgcccc tgaacctgca tgacggctat tttccccttg
    11761 ggagtttcca cgactacgct tccgtgcccc ggtgttccct gcggatagtt gacggggcgc
    11821 agcacccgcg gttcggattc catccacggg gccagctccg cctggtccca gacatggtct
    11881 cccgttgtga tgacatccac tccggcgtgc agcagttcct gtgccagacg gggcgtgata
    11941 cctctcccgg cagccgcgtt ttccccgttg acgatgatga agtccgcccc gtgttcccgt
    12001 ttgagttccg gcaacatgtg tttcacggcg gtgcgtccgg gttcgccgac cacatcgcct
    12061 ataaagagta tggtaatcat ggattgggac ggagctggcg agatggcggt ttttactgtt
    12121 cttccctggt gacgggcact accagaacgg gcacggggga tttccgcatc acggagcttg
    12181 ctacgctgcc cagaaagacg gtggagagga agccgtggtt atgcttgccc accacgatca
    12241 tgtcgatgtg gtgtttttca gcataattaa tgactgcttc gctgatttca aattcatctt
    12301 ccaccgcctg gatgattgtg gcgtccgtca gccgggagag tttttccgcc gtctgtttca
    12361 aatggttttt ggcgatggaa atgacttcgt tttcttccgt ctggtccatc acgggaacgg
    12421 gcacttcatc cggcagaacg ccggagactt catagcaaat gctgggttcc acaatgtgga
    12481 gcaaatggat gatgctgtcg ttggggcagg ccagtttgcc gatttggtgg atgacggcgt
    12541 ccgtggcgtt ggagaaatca atagctacca gaatattctt catatctttc tgtataacat
    12601 tccgtacttt tccatgccac aaaaaaacgt gtgctgcccc ggctttcccg ggtgttccgt
    12661 tttattccgt ttcaggccag ccccacggcg cggcgcaccc tgtccagcac tacagaggcc
    12721 gtctcgcgcg ccttgcgggc gccttcttcc agcgtccggc gcacatagtc ctgattggct
    12781 tccagttccg ccctgcgttc gcgcgcgggc cggaagtatt cccagtaagc gtcaaagagg
    12841 cgctttttga agtctccata gccgcatccc ccgttctgga aaccgcgcac catgtcttca
    12901 taaccctgcg cggaggcaaa gagtttgtaa agctgcagga tgacggaacc ttccgtcggt
    12961 ttgggatctt ccacgggggt ggaatccgtg gggattctca tgatgagttt gcggacagct
    13021 ttttcctctc cgaaaatagg gagcgtgtta ttatagcttt tactcatttt ctgcccgtcc
    13081 agccccggaa caatggctgt gctttccgca atgagcgcat ccggaagttt gcaggtgcct
    13141 tctccgaatt gttcgttcat tttgcccgcc aggtcgcgtg tgacttccag atgctgtttc
    13201 tggtccttgc ctacgggcac caggtcggag tcatacatca ggatgtcggc agccatcaac
    13261 acggggtagg tgaacagggc gttgctgggg gagaatccct tggcgatttt atctttgtag
    13321 gaatggcagc gttccagcag gcccatcgga cagacggtgc tgagtatcca agccagttca
    13381 ttgacttccg gcacggcgga ctggcggaaa aagacgcatt tttcgggatc caggcccacg
    13441 gccagaaagt ccgtagcgag gttgctgcag ttttccctca gcgtgttcgc gtcgtgaacg
    13501 gtgctcatgg cgtggtagtc cgccaggaag tagaaagatt ccccgctttc ctgcatgcgg
    13561 acagccggtt ccatggctcc gaagtagttg ccgacatgca gcttgccgct gggttgaaga
    13621 cctgtgatga tacgcattgc gcgcgggatt ttgcctgttc gcgcccggca agtcaagaaa
    13681 ggggagagtc aaagacaggc ggggccgccg gctggatttt tttccggcat gtgcctgcct
    13741 tacgagatgc gttcagcagg ggtgcctctt cttatttatg gaatgtccgg aaagggccgg
    13801 tcagcgagga aaaggatggg tgtgaaattt actcttccag tgcctccagt acattgtgca
    13861 caccgtccgc aaagcttggg tagagcggtt cccagcccag ggccagaagg cgggagcagg
    13921 aaatcctctg gttggagaag ccccggcgcg cttccgccgg gagcggttca ggctgcggag
    13981 cgggtttccc cagcaaattg gaaaggtagg agtatatttc tcccatttgc atgggggtgc
    14041 ggtccgtcag gttgtaaatg ccggcaggcg gttcccgcag ggtgcagagc aggtgcaggg
    14101 cggctgcggc atcatcccgg tggatgtaat tgagccatct gttcatggct ccggggaggg
    14161 ccttcccctt cgtgacgtac tgggagacga gaacacagcg gcccggcccg tacagggctc
    14221 ccagccggac gaccgtgccc cccgctgcca gaaccgcctg ttccgcctga ataagcagcc
    14281 cgttttttcc ggaggaggga tagacgttgt gttcctccgt aatccagcgt ccgtccgtga
    14341 ttccatagac ggctgtgctg gaacagaaga tgaccggcgt tccggaaaag gcttccagcg
    14401 tgttccgcgt tccatgagcg taaaggttgc ggtaggcttc ctctccgcct cccctcgtgc
    14461 tggcgcacat gacgatgatc tggggggagg ggatgcgggc ggccagcccc tgcatggagg
    14521 cctggtctgc aacgtctgct tcgtaaatgg ctttttgcgg gtcaatatcc gccgtcaggg
    14581 ccgtatgtcc cgctttccgc aggctttccg ccagggcttt tcccagatac cctgttccca
    14641 gaatgagaac gatgcgggga ttcttacaca gaaggccggt catgagtcat tgcggaggtg
    14701 cttgagttcc attagcattt cagcgcggga aatatcttcc ggccatgtga ttttgggatt
    14761 gggctcatga ttatgcacca ggcgcgtagg atggccgatg agttgcaggg cggttacttc
    14821 atccgttacc tgaatattcc gttccgtgac ttccacatag gcgtccagga gaagaggata
    14881 ttgaaagacc tgcggggttt ccatgcacca cagcccgtcc cgttccacct gttccggaag
    14941 cgtaaagcgt tctttgtccg cacgcttcag ggtgtccgtt acgggatgtg cggaagcgga
    15001 cgccccatat tccctggcgg tttgaatgca gcgggagatt tgctccaccg taatgagcgg
    15061 gcgggctcca tcgtgcacgg ccacgaattc cggggtataa agcagggctg ccagtccgtt
    15121 ctgcacggat tcatgccgtt ccgcccctcc gtccacacgg gtaacgggta tttccgtttc
    15181 caggtttgcg ccgttaatgg catgaaaacg ttcctccggg caaacgacta cgatttccgt
    15241 gatttcccgg cagttggcaa aggcgcggat gctgcgttcc agcaccctga cgccgtgcag
    15301 gggggccagc agcttatcaa atccggcacg ccgggaggac ccggccgcaa cgatgatggc
    15361 ggcgcagcat ttcatgacag tctggctgac accatgcggg ccaactcctt gccgttgact
    15421 ctgccggcag tgcgttcctg catgagtttc atgacctgcc ccatatcttt tttggtagag
    15481 gctcccgttt ccttcatcac ggtttccaga atggccgccg tttcttctgc tgtgagttcc
    15541 gcaggcatga attgcttgag aacggtgatt tctgcctctt ccttttccgc cagttccggg
    15601 cgtccggcgg cgcggaattg ttccgcagag tcctcacgct gcttgatttg cttgcggacc
    15661 acggccagtt cttccgcttc ttccagccgg gttcccaagc ctcccttggc gatggccgtg
    15721 ttcgtgagag ccgtcttgag ggcgcgcagg gtattgaggg taacggagtc ttttgctttc
    15781 atggcggttt tcatcccctc catgatttgg tcggaaatcg tgttcatatc tccattaact
    15841 aagcgcatcc ccaggtgtac ttcaagccgt aaattgaaaa aggccgccag cgcggggaat
    15901 gcagccggcg gcccggataa ggggaataaa agcggaacag ccgtgtttat ttttttccgc
    15961 caatgggcat gatgtggatt ttcttttccg tcatgcccgg aaccaccatc atggagtcat
    16021 acaggataaa gtccgccgcc ccttcaacag ggccggtggc taccggggcg gaacgccccg
    16081 tcttcaaatc cagcctgcgg acagcttccg gttttttatc cttggaccag tcactgtagt
    16141 aaagggcgtt gtcacggtaa accagtccgt catactgtcc ccgttccttg gaaagttccg
    16201 taacttcccg ggaaacgggg ttaatggaga gcaggcgtcc gctgggtttg cctttttcat
    16261 ccgttgcgta ttcgcagagg taaatgacct ttctttccgg gtcccaggcc aggccgttcg
    16321 gcttgtagat gggttgtttg gtgaccagtt ccccataaga gctggtattc gtgtccacat
    16381 agaaaatctg gttggtatcc gtggaggaaa ccatgagcct gccattgatg aacgccaggt
    16441 cattgaggaa tttcatgcgg gatggggaca agtccacgta tccttcaata gcgccggtct
    16501 tggcgtttat tttgaatacc atgtccacgt ccgtgcagta aaggaagccg tcctgataca
    16561 gcaacccctt cggcgcgtcc agggtatcta cgtccgtgat ttttttcggc tctgtggaac
    16621 cgtgcggaat gacgcccagg tagccgtcct tgtctttttc cgtaggcgtg agcctggagc
    16681 cgatgcaggt gaaatagata ttgccctcgg catccgctgc aacgctttcc ggatgaccgt
    16741 caatggcaat ggtgtttttt tccagggcgg gcagcggttc aacgggggcc ggctgttttt
    16801 ttgccggcgg cgcggccagc gcgctgcacg ccaggcaaag aaggagtaat gtcgctttca
    16861 tggggtggtg taagagtttt gaaccttatg gcatgggagc aatggaaaag caagccccgc
    16921 agaggctggc aggctgatgg gcggagttgc gccccgttcg ggcaaacggg gcgcttcccg
    16981 tgttagatgg ttcctgcatc cagattattg ggctggggca tgttttccat gcggatagaa
    17041 cggttgaccg cgttcaggta ggcccgggcg gaagcttcaa tgacatctgt ggaggctccc
    17101 ttgcccacaa tgggcttggg ctggccgaag tccacatgca ctgttacttc tcccagagcg
    17161 tccttccctt cggaagcggc gttgacgctg tagcctttca gcgtgccttt gcgcctggtg
    17221 atgcgctcaa tggctttgat aacggcgtcc acggcgccat tgcccgtgga gctgtcttca
    17281 aatttcttgc cttccttgag cagtcccacc gtagccgtgg gccgcgcgtg gctgcctgcc
    17341 acgaattgca ggaagtccag ttcccatagg ccggtggagg catgcaggga gtcgtccaca
    17401 atggcggaaa gatcatcatc ataaacgaat tttttggagt cgcccacttt cttgaagcgt
    17461 tcaaagacgg tgttgacttc ctcttccgtg aggacatggc ccagctgttc cagcctggat
    17521 ttgacggcag cgcgtccgga atgcttggtg agcgggagtt ccgtggctcc ccagccgacc
    17581 gcctgcgggt ccatgacttc ataggtctcc cggcagttga ggatgccgtg ctggtggatg
    17641 cctgaagaat gggcgaaagc gttttccccc acgatggctt tggaccgttg cacctgcatg
    17701 ccgctcatgc gggccacgat gcgggaagtg cggacgattt ccttcgtgtt gatgttgtcc
    17761 gtgacgtctc cgaattgtcc ggcgcgggtg cgcagggcca tgatgacctc ttccagagcc
    17821 acgtttccgg cgcgttcgcc gatgccgtta atggttcctt ccacctggcg ggctccggcg
    17881 cggacggctg ccagggagtt ggctacggcc aggccaaggt cattgtggca atgcacggaa
    17941 atgacggctt tgccgatgtt cggcacattt tccttcaggt ggcggatgat gcggtagaac
    18001 tcgtccggtg tggtgtagcc cacggtatcc ggaatgttga cggtagtggc gccggcatcg
    18061 atgactgttt ccacgacttt ggtcaggaag tccaggtctg tgcgggaagc gtcctccggg
    18121 gaaaattcca cgtcctggac aaattgtttg gcataggata cgccttccgc ggccatgcgc
    18181 aggatttcgg attccgcttt tttcagtttg aactgcatgt gcagcttgct ggtggccagg
    18241 aaaatgtgga tgcgggcacg ttccccggcg ggttccaggg ctttggccgc cgcttcaatg
    18301 tctgccttta cgcagcgggc cagcccggcg atgcggcatt ttttgatttc gcgggcgatg
    18361 gtgtgcacgg cttcaaagtc gccgtcggag atgcagggaa agccggcttc aatcacgtcc
    18421 atgcccagcc gttccatttg acgcgccact tccagctttt cgcgtaggtt catggatgct
    18481 ccggggcatt gttctccatc gcgcagggta gtgtcaaaaa agtagatttg atcggacata
    18541 ggttaatgat gttaagcagt ggcatgataa tgttttgctc cggtaacgca agccctacgc
    18601 gctgcatgct ttcacagagc gtccgggcgg cggaagaggg ccgccggaga gaagaatgac
    18661 ggaaccgctc tttgcttaaa aggaggagct ttgtaggatg gaagggtgaa gatcagattt
    18721 tacctgaatg accaggcgga gcgcatcatg gctctgtgga gcgaaagccg cggggaatac
    18781 ctggcggcgg actccgtgct tgccgaggct gatgcctgca tttgcggaga aacgggttcc
    18841 tgcggagagg aaatgaagcg cattgccgcg tttctggata gtgccgccgc caaagccgtc
    18901 aaccctgaat ggatgattga taccgcactg ccctgttccc cttccaagct ggtgtgcctg
    18961 ggaaaaagtt atgcagacca tgcgaaggag tttgacgggg attccgtgca ggaaccggct
    19021 gtttttctga agtcgccttc cgccattact tcctgcagtg atgttgtttt gtatccgccc
    19081 ggctgcgaca agctggatta tgagattgaa ttggccgtcg tgattaagga tgtgctgaaa
    19141 aacgcttccc cggaggaggc cgcaagggct atcagcggct ataccctgat gtgcgactat
    19201 tcggaaaggg ccaaccagct ggaacatggc gggcagtgga ccaaggggaa gagctatgac
    19261 agctttgccc cgatggggcc cacgttcatt tccgctgatg aacttgggga cggctccggc
    19321 attcctctgg aattgaaagt gaacggggaa gtgcgccaga gcggcaatac ggacggcttg
    19381 atctggcctg ttccgcgtct gattgcctat gtttcccggt tcatgacttt atggcccgga
    19441 gatgtggttt ccaccggcac tccgggaggc gtggggatgg ggatgaagcc tccggtatat
    19501 ttgaaacccg gggatgtggt ggaatgggga agccccgctt ttggcttcgc ttcccaggct
    19561 gtggtgattg atgatgaggg gtaggggaat ggttctccgg ccttgttgaa tgtggagggg
    19621 aagggaaagc tctgtccctt cccgcttctg cgtatttctt ctgtatccgg cgcattgttt
    19681 tcccgtggaa cggagccgcc ggtgctgcgt gggcggcaaa tgacgcctgc gcatgtgggc
    19741 agggcgttgt tttctcttga ttacttcgcc gcggtttagt gaagtggccg cgtgaaaaat
    19801 gtggttttgt acgtggtcat ggtgaccggg ctgacggccc tcttcgtttg gctgaaaggc
    19861 aggaatgcgg aacgtgaccc tgcttcctcc gccatggttg tgtctccgga aggcgccgcc
    19921 gcgcgtcctg ttgtgctggt ggatgtccgg actctggatg agtaccgcac gggacataag
    19981 gagggcgcct tgaatattcc ggcagatgag gtggaacaac tggccccccg gcttcttcct
    20041 gataagaacg ccgtgattat gctgtattgc cgtacgggaa agagagcgga caaggcgctg
    20101 gaagcgctca ggaagatggg ttattcccat gtggaaaacc tgcaccggtt tgaaatgtag
    20161 ctttcggccc ggttgggccg aacggggatt ccgggaggcg ccggccatgc cgcctcctga
    20221 ttcggacggc ggaattttct aacgtaaaag gcacggaatc aatgaggaat aagccgtaag
    20281 caaatgaaat tgaagctgtt cattctttaa ccagttccaa tttcccattg gaagattttc
    20341 ctcccatgga gcggaccctg gctctgaggg agcatccgtt gttggcatga atccccttgg
    20401 gaagggaaag ctgatagaaa tttccatggg aaggcttgcc tgccaatccg gcatgctggt
    20461 cgcgggcgat cactttgccg ttttccagga gttcgacgcc ttctatttcc agagcatggt
    20521 ttccggaagt gaaaataaac cgcactccgc ggagt
//
LOCUS       DAWEMX010000033        20429 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1093_length_20429_cov_12.194562, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000033 DAWEMX010000000
VERSION     DAWEMX010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 20429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20429
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_20429_cov_12.194562"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1727..1736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12786..12795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19183..19192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4742 a         4775 c         5729 g         5153 t
ORIGIN      
        1 ggttctagat tttccttact ccgacacata gcaaaaattc cgtcttcaga aaactcctgc
       61 ttcccgaagc ggaatggtca gcctacacaa gctattctct tttaaaagta aatattgacc
      121 tcggattcgc aacccgtttg tcgatattct tattgtactg ccccaattta tgctccggtt
      181 ccaaaagatg agctgtacag gaccttttgt agagatggat ttttttggat atagcctttt
      241 cgttcagagg gctgcctggt aaatgctgaa catcagaatg gccattcccg tgagggctgc
      301 gcctgcaatg atgccggagg cggtggggac tttatattcc cgtgatttgg ccttgaaaag
      361 atggtccgca gcccagccga gcacagctcc tgtaaagaag aggaaggagt cgttccactg
      421 gatggcccag gccaggccaa agccgatggg ggtggggagc cattttcctg tccggggaat
      481 atagcgggga ataagcacca gaatgagtcc gagaataccg cctgcaatga tggaaagcac
      541 cttgatgctg tgcaaatggc tgaaccccat gcccagtgct tccgccgtgg ccgcccatgt
      601 tctggctccc ggagcattga aagcctggag agtgttcgca tccggaacca tggagcggaa
      661 ggccagcacg gagacgaggg ttcccagaaa aattccggaa aactgggcga tgaattgttt
      721 gcgggggtta gctcccagca ggtagcctac tttgaggtct acgagcaggt ctgccgagga
      781 actggacgct cctgaagtga tattggccgc catcaggttg gctgttacgt gcccgggtgc
      841 aatccctccg aagatgagct gtgtaacttt tcccatggct ccggtgggtg taatattggc
      901 ttctccggtg attcggcaga cgaccagcgc aaggaaaaag gatatgacta ccgcgatgca
      961 gctcatccag tacggaacgt taaaggatgt atgagccaga tagccgagag ctcccagaga
     1021 gataagctgg cctgtcagaa accagctcat gggtgtttct attttttcca catctgtcag
     1081 ttttcgtttt ttactcaggg aaaacatggc ccctagggaa gaaaaggaac gaacaatgct
     1141 tttccattgg aacaggaaag ccacaatgct ggcgacgacc atgcaggcgg ttcctcccca
     1201 cagggtccag cttacgctct cccgataacc tgctcccgcg ggaagcaatt tctggctttc
     1261 cagccacgga acgtaaagag cccagcaaac ggttcctccg atgaagagac tgagggatgt
     1321 tttcatgcct accagagctc ccgcacccac gaacatgaga tcccatttga agaagatagt
     1381 acgctggtac cagttgccga ggatatgttg ctggaccgtt ccaagccatc gttctcctcc
     1441 tgcagccatg gcgatggcat tagcggcggc gaaaagggca gaataaagaa gagctttggc
     1501 ctgtcccgcc gcctttttgc cttcagaata gagaactttg agggtttccg ccgtagcaat
     1561 actgtctggg aaccttattt gttcaatgtt gatcatctgg cgtttcatcg gaatggccat
     1621 ggtgacaccc agtacggcaa tgaaaaatat ccaggcgaag gtagtgccaa gaggcatgtg
     1681 ctgtcctgtc agcaggagca gggcggctac ggcggaggtg agggttnnnn nnnnnnatag
     1741 cctgcagaac tggcagcgga ctgcatgcat gtattttcca gaatagtcat gggggatttg
     1801 gaaatgccca ggcgcacaaa ggcgttccag agggcaaaag agataattcc tgccgtcaga
     1861 gccaccccaa aggaccatcc cattttgagg ttggcgtaca gattagtgag ggaaaggatg
     1921 gagcctagga gcatgcctac gatgacggct ctccaggtaa gctgcttcat tctgtctccg
     1981 gaacccagat aaacttgatc gtaccattgc tgttctactt catccggagt gccttggaat
     2041 ccatccagag gcagcggttt ttccaagcag gaggggagag ggctggaaga ctgggaggac
     2101 gtagatgcca tacagttcat aatatagcat ttgcgggcgt ggtggaaaag tcgtatctgt
     2161 ctttctctgc tttcggcgtt ccttctgctt gagacttgcg tgcttgtgac ggccgtggtt
     2221 taatgggcgg catgagccaa tcttttacgg ataaacttgc cgcccgcatt aaagagaccg
     2281 gttccgcctt gtgtgtcgga ttggatccgc gccccggcat ggatgatttg gaggcgattc
     2341 ctgcgctgtt gcggaaggtt gtggaggaaa ccgctccata tgcggcggcg tttaagccga
     2401 atattgccta ttttgaagcc atggggttgc gcggcctgga aatacttgag gatttgcttc
     2461 cagatatgcc taaggatgtt ccggtggtgc tggatgctaa tcgtggagat attggagaga
     2521 ctcagaagta ttacgctcat gcttactttg aacgtctggg tgtggacgct gtgacgttga
     2581 gtccttttat gggatatgac acgctggagc cttttctgaa ttatgaaggt aaaggtgttt
     2641 atctgctggc agttacttcc aatcccggtt ctgcggatgt ggaacggcag gaattggcag
     2701 ggggccgcag ggtttttgaa ctggtgggag acatggtgct ccgctctgtc cgggaaggct
     2761 gtaaaacttc cgtaggcatg gtggttgggt tgaccaatgc agattccagt attctggaac
     2821 gtattccgga tgctcctttg ctcattcccg gcctgggtgc tcaaggcggt gatctttcct
     2881 gcctgagtgg ttccggtcac gtggcgcctc ccctgattaa tgtctctcga ggaatcatgt
     2941 accagaatcc ggaattgggt tttgcagaga aggccgccgg atttgctcag cgcattcgag
     3001 aggcattgaa ttattgatgt tcatgtattt ttttgcaaat aacaaaatga taacggcctg
     3061 cttatggctg tgcatgagct tgtcaaaggg agggggctga tatattgaac aggtgcatcc
     3121 ccattccctg cccgtttttt atggatgaaa agcccggaat tgtaaaattc actccccatt
     3181 ccacttccag atccgcaatg ttgattgcgg tggcagtgac agtaggtgtt caggtggctt
     3241 tgtgtgctgt gcttgtagcc atacatttgc aggatatgtg gtttccctcc gtccagggtg
     3301 gggaagtgga aatgacgttt gtgtccctgt ccgaagaagt ttctcaggag atggaagaag
     3361 ttcagcctac atctgttgtt ccggttgcag ctccttccac agtcagccct gttccggaga
     3421 ttgcgtcaga tgaagactta ttggcgatga agctgccgga aaatgaagat tgttccgaaa
     3481 tgaaggattt tcttttggat gaagctcctt ccgaactctt gccggaggag ctcgccgtca
     3541 tgcggatttc catgaatgaa gtaaagcgca ggcagcctgt gaagcgcccg gcggtatccc
     3601 tgtcggggcg tccggaaccg gatcagaata tttctcctga taaaaaggtg cgttataaat
     3661 atgctccgtc cttgcccaat tccgtcaatt cttctaaagt cgggaaggtg aatgccgtgg
     3721 tgaaagtagt ggttggagta aattcccatg gggaaccttc atccgtcaat cttcttcagt
     3781 caacgggaaa tgcggaattg gaccgccttt ttatgcgttg ggttagagag aattggactt
     3841 tttatccggc ggaaaaggac ggtgtgccca ttgcttccaa ggtggtggtg cccgtcaggc
     3901 tgaatataga ttaacctgta caaggaaata taaagatgct tccggagaag acagagaata
     3961 ggcccgttta tgtgatcggc caccagaatc cggatacgga tgctatttgt tcagccatag
     4021 ggaatgcgga gtttttaaga actcatggcg aacctggcgc ggttgccgcc agatgtgggg
     4081 aaatgacgct gagaacatcc tgggtattgg aaaaggcagg agtgccggag ccgatactga
     4141 ttcatgacgt gacgcccacc gccgggacaa tctgtcgtcg tgatgtgatt agcgtaagtc
     4201 cggacgatac attcctgacg gcgtatcgaa ggatgacgga aaattccctt caaaccatac
     4261 cggttattga cgctgattac aaccttcatg gcctattgcg ttattttgat ttgttgagcc
     4321 tgctcatgcc tctgaatatg acggagatga atgtgcgctc cgtcttttcc agcctgagca
     4381 atatcgccac caccattgac ggaaaatgtc tgactgggga atccttaagt gaagaagaaa
     4441 cgcagaatat tctgctggtg ggtgcttcca gcgaacccag cgttcgaaca aggctcgcaa
     4501 attacaggag aaaagggatt gtccgggatc tgattgttat ttgtggggac cgtccgaatg
     4561 tgcagctata tgcagtagaa catggcgtac gcgctttggt gaccacgtcc ggagctttcc
     4621 cttccttgga cattattgag acggcccaga cgacgggaac atgtattttg agtactccct
     4681 gggatacggc cagtgttggc cagttgatcc gttgttcccg gaaggtgagg gagcaggttc
     4741 agaaggatta caccgttttt ccggagaata tgccgttgcc cgagttgagg caggctgccg
     4801 taaagcgcaa gcaggcattg ttccctgtga tgaatatcaa gacgaacaaa ataattggtg
     4861 tactgagtaa gacggatttg gtggatccgc cccgtacacg tgtggcttta gtggatcaca
     4921 atgaattttc tcaggccgtc aagggggtgg aggaggcgga gattgtggag gtgatggatc
     4981 accaccgttt gggtacccag ctttctacgc gggatccaat ccgttttctg aatgaacccg
     5041 taggatccac atccaccttg gtggcacgca gattttacca tcgtaatgtg gaaccctccc
     5101 aggctgtagc ggtctgcctg tgcgcgggga ttctttcaga taccctgaat ttgacatctc
     5161 ctacggcggc tcgggcagat agggaaatgt tggaatggct gacaggtatt gccaaaatag
     5221 atgccaagaa gtttacagag gagttttttg ccaccggttc tctgttgcgt tccaaaactg
     5281 aaccttccgt tattattcag gcggacagga agacttttac tgagtacggg cataagataa
     5341 gtatttccca gattgaggaa attggtatgt ttggcttgga agatgtccag gaaggtttag
     5401 tgagggaact tcggaatctg gtggagaaag agaggctgaa gcttgcttgc cttttggtta
     5461 cggatatcgt tactcacgat tccatgttgc tggccgtcgg ggatgaagaa gctttggaaa
     5521 atatcgagta cgagcggctt ggccctaatt tgttttccgc tactgatgtg gtaagccgaa
     5581 agaaacagct ttttcctgct atttcccgtg cattaaagac gttgtagatg caggaaatct
     5641 ttgcttttta caggcaagat ttttcctgag gaggaacggc gtttctgtgc agtaaagggt
     5701 atagatcagg gaattgcaat gtctgctacg atagaacggc ggatagtatc cggtatcagc
     5761 acagtactgg ctctttcagg tttgagcggc ggcgtgcaaa aaactcggtc taggcgtaat
     5821 agagggaaat tgacaggttg cgtagctccg taaccggcac ctttcagttt gaagcaatcc
     5881 tgaaagtcgg aactgagttt ggagaaaatg ggggattgag gggcggcgct gaagttcccg
     5941 gccatgataa tgggaagttc tccatgctgg gttcctactt ccctcagggt atcaaacagg
     6001 tattgaagtt gtttacggtg gatgaagcgg agtgtatgga aatatttcca gcaggcgggg
     6061 gagtacaggt cgaagcgatg ttcaaaaggt tccaggctga tgttgatgag ccgaatcgcc
     6121 aaggaggaat tttccggtat ccagtctaca atcaaaccgg ctgtgtccgt gatgctctgg
     6181 gctggaccaa tctgaccgtg ccttacaatg atcgcgcaac ttcctatttg cttgatataa
     6241 gaggttctac cgaaaatgct gtaggcaaat ttgagtgttc ggttatagtt gctgcagcct
     6301 tggacgaaaa tgacatctgc acggagtttg gatatttgtt caatgggagg aggttcctcg
     6361 cagccctcgt acaaggtaag aacccttatt tgcctggcat ccggagatgg cggcattttg
     6421 tagtattcca ttccagttct ggcaatgggc gggagaaagt ctgtaatgac gatgccgtac
     6481 gcaacccaga cgataatgcc aagcagaccg atccgggaac cgaagaccag ccatgcaatg
     6541 agcgccggaa aggagaaaat gagacagcaa gcccatatgg gcagtgacgt tgcaaaagcc
     6601 atcgtgtctg aaccggtcca gaaaatagtg acgacgatga cccataggca cagagagacg
     6661 ccacccagcg ctattcccaa gtggttgact ccggatagcg ttggcagagg ttctgccaga
     6721 tgtttgcgtc ttctgagccg atgcactgtg gtgacagatt agaagcccat gcctggaggg
     6781 agatctaggc ctcctgtcag ctttttcatt tccgccgcgg ctgtttcctt acccttggca
     6841 atagctgcat tgatggtttg aaggagaagc tcctggagaa attctgaatc ggacgggtca
     6901 atgatggaag gatcaatgac cagttccgtg aggttgccgt cacaggtggc ggtaactttc
     6961 aattttcctc cagctccttc cgttgtaacg gtttgggagg cgagtttttc ttgggcggcc
     7021 gcaagccctg cctgcatctg ctggacctgt ttcatcattt tcattatatt catggtcgtt
     7081 gtgtctgggt ggatgagttg actattggga aatgatacga gcccggaaaa tctccatagc
     7141 agcatcaatc agcggatccg tgtagtaaga gttatcttca ttttcctctt tggaagcttc
     7201 ctggacaggc tctgcggttt ttttctgcgg ggtatgaggt ttaggtgcag gaatggtgga
     7261 tgcggaagga ggtggtgcag gcagaggagc cagttcttcc tgcacgggtt cgggaatgga
     7321 gaaatcctga cggatagaaa tagtaacaga ggttccaata tattgggaaa gagcggcttc
     7381 caaagctgtt cgcagaggac cggctccaag actgtccatc ccctgacggt cagaaggatg
     7441 aaaagaaatg gcgactgcta cgccatcata accggaaaaa acgctgttgg ctagaaagtc
     7501 cgcttgaaga gggaatttgg cggacatttg ttccaacacc gttttccagt cttcttccgt
     7561 gattgttctt cctgattgtt gggacagaac tgccggttcc tctttttcta cgggtgcctg
     7621 ggtttgggat gatgagcttg gagtatcgaa taggttgtca aagaaactgt tagttcgtct
     7681 ttccagagga agattttcct ctggatccat aaaagtaggc tctggagaat ccgtaggaaa
     7741 tttcgacaca tcggaggcat cggagtctgg atgccgggtg atttcttcag aggcaggtac
     7801 ggtttttgtc cgcaatttag tggcggagta gggaaattct tcctctgcaa cggcaagaga
     7861 agaggggggg gcagcgggtg taagagtttc ctgttgtggt tcggcttcct gcgcaggatg
     7921 gggagaagct tgagcgtcag tgctctgaat tttctgtact gcgggagatg aggcagggga
     7981 ggacacagaa aaatctggaa tcggatccat caaatgttct tccgtagctg gggttgggtt
     8041 tgttgcagca acaggagact ggatgtttgg ccggggggcc ggagtgggtg ccgtggcgga
     8101 ggaaataaag gtacttggtt cctgctgtga agcaagatct gaagaagagg ccggtgccgc
     8161 ggtggctagc ggagcacctt ccaacgccat gataatatcg ctgatgctgg cctcagccag
     8221 agtatgaacg gctttaatca gacccatttc cagatgaagc cttttattgg tggaccagcg
     8281 cattttatct tccgtttccg ccagaacttc caccaaacgc aggattttgt ccgtatgggt
     8341 gcgtttgacc aattcggcga gttcctcctt ggaggattcc gggagacaat caaaactggc
     8401 ttctggatct actttagaga ccagaatttc acgcacggcg gagataattt cggaaaggaa
     8461 ctggctcatg tctcttcctg cttccgcctg ttcatgcaga agatgcagga gagagggaag
     8521 tttcttgttc aaaataagcg ccatagcatg ggccacggtc tcccgggaag taatgccgaa
     8581 aatgtggagt acctgctgtt cttcaatatg atctccacag aaggaaacaa gctggtccag
     8641 catggattgt gcatccctca tacctccatc cgccactttg gcgacagcaa aggctgcatc
     8701 cctgcttaga cttactccct ctgcagaagc gatatggagc aggtgttctg caatgatttc
     8761 agaagggatg ggacgcaggt cgaagcgctg gcatcgcgat agaatggttg gcagtatttt
     8821 gtggggttcc gtggttgcga aaataaattt gacatgaggt ggcggttctt ccagcgtttt
     8881 gagcagggcg ttgaaggatt ccttagtgag catgtgcact tcatctatat agacgatacg
     8941 gaaatttcct ctgctcggag cgaaccgtac gttgtccctg agatcccgga tgtgatcaat
     9001 gccgcggttg gatgctccat ctatttcaag aacatccagg ttccggcctt ccgcaatttc
     9061 ttcacatata tcttcatgcg ggtcaaaatc cactttgggc ccaccgctgc agtttagagc
     9121 tttcgcaaaa attcttgcgg ttgatgtctt tcccgttcca cggggaccta caaagagata
     9181 ggcatgggcg agacggttgt gctcaatggc gtttttcaac gtccggacaa catgatcctg
     9241 tccaaggacg tcatcgaatg tcagaggacg gtattttctg gcaaagacct ggtaactcac
     9301 ggttcagaaa gactagaaga tgagttcccg tttgaaaaga aaatagagag taattgtttt
     9361 aaaatgttga taaaacggtt cttgcgcact cccgttacgg agcttgcctg aaaaaaatat
     9421 caagaaaaca atgaggatgt cattttgata ttgaaatctg aaaatgttca tgccaatctc
     9481 acaaaagtaa caggaacctt ctcatctcta taatttccta tgtgggtcaa agtatcaata
     9541 gcagccttgg tcattgctct tctcggagga gccatgtatt atcttgataa cgaggaagct
     9601 ctaaccaagg agcgtaggga atgtgcttcc cgctacaaga acctgaacgg ccttcgtggc
     9661 gaatttacgg aagaaaagac gaaatatgtc gatttcctgg tgaagaatga ggctctgacg
     9721 aatgacatca atgctttaac caaggagaag gatgaactgg aagtaaagaa tcaggaattg
     9781 gcctcagcca atgaggccaa gaaatctgac cttcaaacgc agcaaaccgc tttggcggaa
     9841 ttgcaatcca agagcaagga tatggaaagt atccaagcca ttgctgacag aatcaagggg
     9901 ctggaagaag agtccaagca gttggaagtg gttaagcagg cggaacaggg aaaacatgat
     9961 gccatcgtcg ctgaaactga acagcttgtt gtgaataatg ccgctcttcg tcagctcaag
    10021 gccgaccagg atgcccgcct ttctccgccc aacctgaaaa cgagggtttc ccaggtgatt
    10081 catgatttta atgtagttgt gattgacgga ggagccgccg atctgggcgt ggtgcccggt
    10141 tccaagttgg ccgtcatgag ggacggcaac aaaattgccg agcttgacgt aaacgctgtt
    10201 gaatcccgtg tttccacggc cactattcta ccaagtacgg taaccgccgg cgaacgtgtt
    10261 gaagccggag acgtcgttgt atctgttcgt ccctagcatt tttccggttc aatacactcc
    10321 atacactctc acatacatac acaatgaaaa ctgtcttagt agttttattg attcttgcag
    10381 cagggacggg cgcctggttc tccggtctta gcggcctttt cggtattaat ggccagtacc
    10441 aggattttaa agaaatgatt tcgtatcgta agaccttgga caaggaaata cgtacttgga
    10501 caagcttgcg caacgacatg attaagaatc gtggcttggc agcggaagaa aatcttagcc
    10561 tgatttccaa caaggcttcc gtgacgcagc agcgtgatga acagctttcc aaggaagcgg
    10621 agttgaagga agaagaagtg cagttgaatg cggacttgtc caaaatcaat aaacagattg
    10681 aggaattaaa gagcaagttg caagatttcg gtgtttccag cgtacaggaa atccaggaga
    10741 agatggaatc tttggaagct tctaataaga agcttcagga agatattgac cagattaaaa
    10801 gtgctacgga agtggcttcc aagaaacggg cagaacaagc ttctgagctg gctagccgcc
    10861 agaaggagca agctgaatac cgagctgctc ttgccaaaaa tggggaagag tatcccgttc
    10921 tatccgtgga cccgcagtgg ggttttgttg tgattggggc tggacagggc agtagtattg
    10981 atccaaatac ggttcttctt gttacccgtg aaggtcgcag cattggtaaa ttaaaggtaa
    11041 cttccctgga gaaaaatcag acggtagctg acattattaa agacagcgta ccatctggta
    11101 tgagtattca acctggcgat agagtacaac tcctgcgtcc ccgtcagtcc gccaaataaa
    11161 ggtttgccac gtattttcct tgaaccccgt tgtgtcgtcc ggcataatgg ggttcaactt
    11221 tttaaaagga aatgatgaaa cgatttgcaa tctccttttt gtttgtgtct ttttctattc
    11281 ttctggtctc ttcctgttct tggttgatgg aaccgcagga gagggagcgt gtgccacagc
    11341 cgcctcccgg tccggaaaag agtgctcttc ctcataataa gacccaaaga tttgaggcgg
    11401 aagcaacgct cgggccgttg gcgtcgccga gacgttaacg gcatccgaat gacgctggac
    11461 ggtgattctg ttctttacag tatccgacaa accggataca accgcctccg gtttccaagt
    11521 ggagagatgg ctgagctggt ctaaggcact cgactcgaaa tcgaacgtgg gctttaaaac
    11581 ccaccgtggg ttcgaatccc actctctccg ccagtttttc agtaagagcc ccgtcggaag
    11641 acgaggctct tattttcgat aggaagggaa cagtcaggaa ttcagatagg cggctgttca
    11701 gtgatagagg gtgcgttctt gaccggggtc tttaaggcgc tctccgccag gatggttgta
    11761 actataataa tttttgttgg ttgtttcggt cggtttacca atgattacat gatccagaaa
    11821 acgaacgcct aataatttgg agcattcccg cacgcgttcg gtcaggaggt cgtccgtacg
    11881 gctgggggct ggatttccac tgggatgatt gtgggcaagg atgaagccat aggcgttatg
    11941 aatgacagtg gggcgtaata tgtcacgtgg atgagcaatg gattcgttca aggtcccgct
    12001 ggatatttcc acgggtttga tgaggcggca cttggtatcc agcagcagaa cgagcaccgt
    12061 ctccttttcc ttccagcgca tggctgtagt cagataatca tagacatctt ccggtgtttg
    12121 aatgggttga cgtgacagag tttctcggag agccctcgct cccagttcaa aggcagcgct
    12181 cagcgtagcg gctttcgcca tgcctattcc cttgcaaatg ttcgctattt cagcagcttc
    12241 catatgagct agggcatcta gagagccagc agactgaatc agatccgagg acatgtccaa
    12301 cacatttttc cccttgatcc ccattcggag aaagatagca agcagttccg cgttgctgag
    12361 gctgtgcctt ccttggcgga gcaatttttc cctgggcaga gactgagagg gcagttcctg
    12421 aagtttgcgg taggtcattt caataacaga ggcgtgcggt agaaagaatg gcggaaggca
    12481 gatgtcgcag cgtatcccct gccgtcaatg tttgctgggt ggcttctccg gcggagattg
    12541 ccatttcgga agctcggcca cacaggtaga cgcccagaac ggcggaaacc agcagggatt
    12601 ccccctggct tataaagcct ccgcaaatac ctgccagcac gtctccctga ccggcagttg
    12661 ccatgccggg atccccagtg atgttataaa aaagaggttt tccccgttgg gtgacgatgg
    12721 tgcgtgctcc cttgtaaacc agggcggctt catgttctgc cagaaagcag tcggcaatgt
    12781 ctgccnnnnn nnnnnccgga tggcatagtt gtctgcatct ggcagaagtc tgcgaatttc
    12841 tccatggtgg ggagtagcca gaacgtgttc tcccaagttc cactgcatgg ccgcggccag
    12901 attcagcccg tccgcatcca gaacagtggg ggtacctgtt tccagaatga ggcggacggc
    12961 ttccgcatct tcctccgata cggaaccgat acctgggcca atgaggaaag cactgaatgt
    13021 gcgaatggag atatctgcgt aactgtccac gggtctgacc atgatttccg gaggcatgga
    13081 tggagcgatt aagggataga cgtttttgtg aacgtgcagt gtaaccagac cggctcctgc
    13141 ccggagagct gcttcacagc agagccgggc cgcacccagc attccttcag agccagctat
    13201 gacgccaatg tgcccgcgtt tgttcttata atccgtatag ggtcttgcag agaggaattt
    13261 ggtaagccgt gaggaggtaa taagttccgt ggcggggagc gcctgtacgt gaagaccggg
    13321 aaggtcaatg cagactaagc gtccggcaaa cagggtggcg tcatcgtcca gcaggccctg
    13381 cttaacggct cctatgcaca tggtgaaatc tgcttccact gcattttgct gaggcattcc
    13441 cgtatctgga tccactccgg tggggatatc aatagccacg gtacgtacag ccccgcaacg
    13501 gtttcttata tagttcattt ccgcgcagag agcggagatt tccctgcgga gcattccctt
    13561 tgcccctatt cctagcaggc cgtccagcag gatcataggt tttcccgtac ggggaagggg
    13621 aggggcctgg tattcctgag ggggaggaga tatttccgcc aactgccgca cggaaagttc
    13681 gctccattcg ttgcgcgggt aagcggttcg gaagcctatt tcccagccat gttgtttcag
    13741 tatgttaagg acggtgaggg catctccccc gttattccct ttgcccacat aggctacgca
    13801 caatcccggg tcggggaaga actgcatgag agattccgca ataccggaag aggccagttt
    13861 catcagggtt ctggctggtg ttccgctaac gatgagctcg cgttccgcga tctgcatatt
    13921 ttccgtggag caaactttca tggcatactg atagaggcag cctttactgt aagctaaaaa
    13981 accttctcct gcaagccgga gaaggttttt aaaaagaagc ggatcaggga gcagctaacc
    14041 gatgcggaac gtgatgcgcg ctttggtcat gtcataagga gacatttcca tacgcacttt
    14101 gtcccctacc acgattttaa taaagcgttt gcgcattttg ccggaaatat gggccagcac
    14161 ttcgtgcccg ttgggaaggc gcaccttgaa catggttcct gccagtacgg cgcagatggt
    14221 gccttccact tcaatttcac tttcggagtt ttcttccttg ggctctgatt tattgaaatt
    14281 ctttctgccc ttggtgtggc gtcctccgcc tccttgcggg cgttgcctgt tggcgttacc
    14341 tccggggcgg cttcctcccc ctgttctgga atttcccggt cctgcagact gtccggccga
    14401 tcgtgccgct cctgagccat tgccggaagg cctggattgt ccgggacggt tactggtgcc
    14461 ggtcggtctg gaaccggggc gggaattttg tccgccgctg gggcggctgc tggatcttgg
    14521 atttgggctg ataggactct ccttgtaaag ttaaaatgaa cgcgtgtgta caaatcccgc
    14581 gcaggcgaat gatagaccat atttttgcaa gggcaagtcc tgaatgcgga agttgtgcaa
    14641 ataaaacaca attctatgga gagggacggg aaaaaggctg taaaagagaa aaagtatttg
    14701 acatttacgt gaataatcat taggaatgcg cccccgaatt ggaagataac ggcaggatgc
    14761 cgaaaaacga gaacgaagaa ttgaaccatg aagaccttct cagccaaacc gcaagaagta
    14821 gagcgcaaat ggtatgttat agacgctgcc gacaaggtgc tcggccgtgt tgccgttgaa
    14881 gcggctaaca ttttgcgtgg caagaacaag acaattttca ccccccacgt tgattgtggc
    14941 gattttgtca ttatcgtaaa cgcggacaag gtggtgttga ccggtaacaa ggaaaatgcc
    15001 aaaatttata cccgtttctc cggttacgtt ggcggtaagc aggtggacac cccccgtaag
    15061 attcgtgctc gccgccctga acttcttctg gaactggctg ttaaaggcat ggttcctcac
    15121 acccgcctgg gccgtcagca aatgaccaag ctgaaggttt atgccggcgc cagccatccg
    15181 catgaagccc agcagcccac tgccatcact ctctaattga tttttcagac atgagtcaga
    15241 caaccccata caacgccacc ggccgtcgta agaccgccat tgcacacgct tggcttactg
    15301 aaggttccgg ccgcatcacg atcaaccgcc gcggttttga ggaatatctt cctaccgttc
    15361 agttgcagaa tgctgttctt cagccctttc aggttaccaa taccatgaac aagtttgatg
    15421 tgaacgtcgt taccaagggc ggcggtattc acggacaggt gggagccatt cgcatggcga
    15481 tcgcccgtgc tctcgttcag gttgatgaag cttcccgttc ccagcttcgt gaattcggtc
    15541 ttctgacccg tgattcccgt atgaaggaac gtaaaaagcc cggccgtcct ggagctcgca
    15601 agcgttatca gttctccaag cgttaagctt ggctggttgc gaaggaactg gctttaccat
    15661 ttctttctcg gaaaaggagt actccctgtg gagtgctcct tttttgttgc aggaagaact
    15721 ttgcttttta ctgcttcacg cgtttttaaa aagctttttc tggttgggtt gcttacggca
    15781 gctttcaatg attttcctgc atttgctgtg aaggggggga ggtttcccag tgcagtaatg
    15841 gttggttgac gagggttacc gatggtatgg tgttcctgcc aggatagtgt ggcagcggta
    15901 aagctgttcc agcaggacaa caagcgccag ttcatgttga agggtgaatt tgctcaagga
    15961 caggatatga ttgcaccggg accgcaattc ttccgtatga ccgtctgagg ctccgatgag
    16021 aaacgcgatg cggcgtacgg aatgcatttg ccagtccttt gccaggtcga ctagctttct
    16081 tgtggtccaa agttctccgc gttcgtccat ggcgatgcgc aggcaaccct cgctggcggc
    16141 cagcagacgt ttagaaacgt cttgggagct tccgtccctg acaagcttga gttccgtttt
    16201 gccgaagggc ctgaggcggt tgagatagag ttccacgcct tccttggcgt agcctaggga
    16261 aggctttccg gcggccagaa tcaggaattg catgagaagt caggagttgg aagatgagtt
    16321 aaggtagttc agcagggtgc gggcccaagt ttccgaaatg ccgcggatgg cggcgataga
    16381 agccgggtcc gcctttttaa tggcagttac ggatttgaat ttttccagca acagtctttt
    16441 ttttgaggcg ctcatgcccg gggcgtcgtc caaggcgctt tcccgcatgc gtttgcggta
    16501 gagcagttcg ttatagccgt tggcgaagcg gtgggcttcg tcgcggatgc gctgcatgag
    16561 tttgagggct cccgtgctgt gaggcaggca aaggggctgg gactggtggg gaaaaaatat
    16621 ttcttccctc tgcttggcta ggcctacgat gggcatgtct cccaggccga tggcttccaa
    16681 atcggccaga gcggaggaaa gctgtccttt acctccgtcc acgactacca gatctcgaac
    16741 tttgatgggg gcttttcctt ccgcgctgag tttcttaagc cactggtaca gggtcatgtc
    16801 cgcctgccgt gaggctacgg cgtcgctctc cgccagaatg cgggagtaac gcctccggat
    16861 gacttccgac atggaggcga agtcattttg tccgtccaca gtacgtatac ggtaccgacg
    16921 gtaggctttg ttatccggcc tgccgttggt aaagcgcacc atggaggcta cgatatggtt
    16981 ggaagagaca ttggaaatgt caaagcattc catgatggtt gggggagatt ctaatcccag
    17041 ggccaccccc agttccttca tgttttcttc cggatgaacg gtgccgggaa gatcgggagt
    17101 tccttttcgg aaccgccggg cgggttccag taccttgacc agattgtccc ggatgtcccg
    17161 caggtatgcc gcacgttcaa agtccagttc atcggcggct tccatcattt ccctggtaag
    17221 ttcgtccaga gtgctttttc tgcccgttcc ttccagcaga cgcaccgcct ggtcaaagtt
    17281 ccggttgtaa tcattgatgg aaatgcggcc aatacacgga gcggagcagt tgcgaatcac
    17341 atccgcatgg cagtgcctga agtcatggat tcccggattt tttgtcttgc aggttcgcag
    17401 ccggaaatgc cggttgagcc attcctgcgt ggcgtaaaga gcctgggagt ggacaaaggg
    17461 gccgaagtag cgcgctccgt catctttgcg cactcgcgcc agctggaaac gaggcagttt
    17521 ttcccccttc ggaattttca gcagggggta gcgcttgtcg tccttcatct ggatattgta
    17581 atgcggacga tattgtttaa tcagtttgct ttccagcaga aaagcctcct ggtcatttct
    17641 ggtttcaaaa taatcaaatg acgcaatagc gttaatgagc gctcttgtct tatgattgga
    17701 gagtgtggtt cccgtgggag aaaaatagtt gcccaggcgg cggtgaagat ccttggcttt
    17761 gcccacgtag atggtatttc cccgggcgtc tttcatgata taaacgcctg gtttgtgcgg
    17821 agtttccctc cagagttctt tcagggttgg tttctcgcgg tctgccacgc aggcattatg
    17881 ccatatgagg caagaaatgg aactgcggaa acgtactgta ttacggctac ttggcttgcc
    17941 taggaaaact tcttctgtat aatcccctat gtcttttgta tgaatcctgt accgtttacc
    18001 acgctggctg ccgccatgga ttttgtagtg ggagatagaa attatcccct ttccgaactg
    18061 ttcatgaaag gcggtttcat catgtggccg ctgcttttgt tatccatcgc cggagtagtg
    18121 gtgctggtca tgtgctgttt ttctacccga gcttccgccg tcctgcctac caaactggtg
    18181 gaacaggcgg aatctttcat tcgcaaacgg gattacacgg gactttccat cctctgcaag
    18241 aatggagatt cctgctacgc tcgcgtgatg ttgactgtag ccaacttcat gttgcgcaat
    18301 ccctccgccc agtttgagga ggtaagggag atagcctctg cggaaggagg acggcaggcg
    18361 gggttcctaa gccgccagat ttcttggctg tcggatattg gcgccgtagc ccccatgctg
    18421 ggtctgttgg gaaccgtggt cgggatgatg aaaacctttt ttgagattgc caacggtgat
    18481 ttttccgggg gcaagcagcg catcgacatg gcgggcggcg tggcggaagc cttgattacg
    18541 acggcgggcg gcctgatgct tggcattccc gccatcctgg cctatgtata tttccgcagc
    18601 cgtgtacaca aacgggtggg agacctggag gcagccgtca cgcacagtgt ttccgtagtt
    18661 gccacccagc ttcagaagcc acgagccggt gcagctgggg cttttcatga agaggaactt
    18721 cccaaagtcc ccgtagatcc cacttccctg cgcgatgtgc gtgggatcta gccggaacgc
    18781 atccacttct ccacctcacc ggaagaatgt atgaaatttc attataaaat gcccagtccc
    18841 atcgggtttc aattggcccc catgctggac atcgtatttc tgctgctggt gtttttcatc
    18901 gtaacccaga catttgagga tgatgaaccg gatctttcca tcaatcttcc ttcagcagag
    18961 actcccaagc ccggtgaaaa cgtgtccaat gaaattatcg tgaatatccg taaagatggt
    19021 accgtggtaa tcaaccgcca gcagtacaca atgccgcagc tggaagacaa acttttttcc
    19081 gtcgcccggt tggacaaaac catgctcgtc cgtatccgcg tggatgagaa ggcggaatcc
    19141 ggcgtggtgg tgcatgtgat ggacgcatgt ctgaaggccg ggnnnnnnnn nntctttttc
    19201 cacgcgtcct ccggctccat cttccatcaa tgtttccaat tcccaggctt catgaaagcg
    19261 tttttttcca ttaatgcagg tttaataact ttggcgatgt tttccgccgc ccaggaaaat
    19321 attccggatg ccgctcccat cgcggatggt ccgctggtcg ctaatccgga gcaggatacg
    19381 ctggatatgg cggacatgct gtacaagcag gcccaggctc cggctacgaa ggagaaccgg
    19441 caggaatatg gacgccttct tgatttaagc ttgcgtaaat atctggaatt tacccaacgt
    19501 ttcccccagt ccgcccaggc tcctctggcg gagtatcgtg ctgccatgtg tttggaggag
    19561 ctgggcagga aagatgaggc acatggcctg ttcctcagat tgattcagac gggcagcccc
    19621 gccctggttg ccgcctccgc ctaccgtctg gcgacggatg cttcttctgc cggggagata
    19681 gataaggcca tccagtatta ccagctcgtg attcgcaatg cggaacagaa tgatttgaag
    19741 gtggacgccc agtaccgtct gggacgcctg tttctttcca gcggtaatcc ggaggcagcc
    19801 gccaccatgt tttgtgcggt gatgggcaat ccggaagcgg atgccaagtt tgttctggtg
    19861 tcccggatgg gatatgccgc cctgtgtgcg gatacgggac gcttgggaga agcttattcc
    19921 gaatatcgca aggtgctgga aacgcccggt gtggataaca ggaacagggg aatcgccact
    19981 cttcaggccg ccatgctggc caccaaactg aaaaagactg cggaagccca gggcctttat
    20041 gaacggcttt tgaaggacga gtccctgaag gagatggcgc cggaggcccg aatggggctt
    20101 cttctggggc tgtacaatat gggcaagtac agggagatac tttcccagta tgagcagcag
    20161 aagggaataa agatgccgac aaaggacggc caggtgcgtc tgttgatgct gctggggcag
    20221 tccgcctata aattgaagga gtaccggaaa gcggcagatt tctttctgga agcggaaaag
    20281 tccgttcctt atacgcagga ggccatgcaa gcctcctttt accggttatt gtgttataat
    20341 gaacttaagc agaaagatct cccccagcgg gcccagagct tcctgaacca ttatgccaag
    20401 gcttttccta ccagtgagct gcatgatat
//
LOCUS       DAWEMX010000034        20228 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1110_length_20228_cov_11.391067, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000034 DAWEMX010000000
VERSION     DAWEMX010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 20228)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20228)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20228
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_20228_cov_11.391067"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    230..239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9746..9755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4510 a         6208 c         5366 g         4124 t
ORIGIN      
        1 ctgtacaatg tagtgaaagc caaaacatcc gttcccggca cctacttttg cgccatgaat
       61 tttgacgacg gctatatcgg ttttcaggaa caatccaacg gtaaaaaagt aatcatcttt
      121 tccatctggg atccggtggc acacggagac aaccccaatg atgtgccgga agaagaacgc
      181 accaagctgg taaaactggg caaagatgcg cgctccggac gttttggcgn nnnnnnnnnc
      241 atcccaatac cagattaacg gtcaccggcg acccaacccc ctccaccaac gtcatggcga
      301 acatccagcc tgatggttcc gtgctgcttc agacgggggg aactacgaaa atgacggatt
      361 tcaagctgtt ccaaaatcgt cccctgcccc agaatatcaa gctggtcctg cctgacaagg
      421 ccgttaccag tcttgttcag gagaacatta actgattatg tcccccaccg gctggctaac
      481 catgattgtt tccatcggcg gcgtactggc cgccgccgct tgggcgtatt acaaagtact
      541 ctccaccccc aaggaaacgg aatatctggg cagccccacc ccgccactgc cggaaaatgc
      601 ggaggaataa ggccccgacc cggtgaaccg ctgcactccg gactgaacaa ctcccccaaa
      661 atcatcttgc cgcagccaga gagagcgcat gtcgtcccga tacctgaaac agggcggata
      721 taagggagta tttcccggca gacaggcaac atggaccggt attctccggg gatatgcacg
      781 caaaccgcgc ccgaatcatt cgggccgcac cggtccacag gatttttaaa cggttcttct
      841 ccttatccgt ctgacggagg caagatgaag cccagccggt caatgccgta tctggcgcat
      901 tcctgccaca gggatttttc cagggcttcc gcagtacggc ggcgttcatc ctccggcatg
      961 ttttcaaaat attccggccc caggtgttcg ctgatggcct tgtccgccat acgcgtcacg
     1021 agatccgccc aaaaagagtt gtcccggtat tcatccaggc aatcctggaa aaaggcgctt
     1081 tcttcatatt cccgggtgaa ggcatatccg cccagatcat ccgcataggc aatgcgccct
     1141 ttcaatttgg aggtgacgga aagctctttc atcagccggg aaaccaaatt cccccacgcc
     1201 gccagacgcg cctccgcccc gtcctgctgg ttggaggcag ccaccgtggc ggcagtggaa
     1261 agcatttccg ccaaatcacg cgcttcctcg tcggacaatt tcaacgtcac cggcatggaa
     1321 aatcaaatca atccgagttt cattctgccc tcctcgctga ccatgtcctg gttccaaggg
     1381 ggatcccaca ccatttccac atccgcagcc tccacccctt ccaacgcttc aatgcggtcc
     1441 ttggcttcca tgaccagatg gggccccatt ccgcagccgg gcgccgtgag agtcattttt
     1501 accgccacat gatgaaggcc gttctccagc tcaatgacgg ccacatcgta gatcaggccc
     1561 aagttgacga tgtccacggg gatttccggg tcatacacac atttcagcgt atcccagacc
     1621 ctttcttcca gtgtggcgtt ctcatccagc gcagccgctt ccggagaagg ttcctccgct
     1681 ccagcgccaa gggcgtccgc gttctccctg gagatacggg ccaggccgtg gtccgtagcc
     1741 acggtatagg agccgcccag tatctgggtg atatacacgc gttccccttc cggcagggtg
     1801 agcacatccc cgctggggat ctggaccgcc gtcacttctc ttttgagttc tactacctgg
     1861 tttagcataa atccttgcaa ttaaaggaat atccggctaa ggacatgcac cggaggcttc
     1921 cgaatgccat gggacagaac cgacccatgg tttccgggac cttccgcgca ggagcaacat
     1981 aaggcgtatt tgcatcaccg tcgaggtcaa aaattgttca gccccggccg gaaaagccct
     2041 tcatgacagg aaagagcatg ccgccatgtt ctggaaaagc cgccggaagc ggagagcttc
     2101 gttcttgcct agcggaggga aaaagtgtaa aaggttcggg ctgcccgggc tgccgtcagc
     2161 tcatggccgg ctacacgtac attttcaacc tgacattcca tgctagatta cttacgtaaa
     2221 catacaatcg tcatcatggc agccatggcc ctggtgtttg tgggcctcat gttcgtcggc
     2281 ggggacgtaa gcggcggctc cctgaccggc atgttcaggc agactttcgt ttccgtggac
     2341 gggaaatctt atggggaaaa ggattataac aatatgggca ggaccggcct gtccgtggct
     2401 ttctccttcc cttccacctt cggtccgctg cttcaggata atttcagctc ggaagaaaac
     2461 ctgcgcgaac tctgccatct gctgaaaaca aataatccca atgcggcttt cctggcctac
     2521 cgcggcatta tccgccggga ggcgggacgc ctgggcttga ctcccagcac cgcggaaatt
     2581 gacgatgcca tttgcaatat tccagagttc caggacgata aaggcgtttt cgacccccaa
     2641 aaatacgata atttcatttc catgcgcggc aacatgggta aaaagcttca ggaggaactg
     2701 ctgcgcggtc tgatggaaga caccatcagc ctggagcgca tcaaggacgt gctggccggg
     2761 ggtatctccg tggagcccgg ttttgccgag gctgtagcgg aatccaacag ccagcaaatt
     2821 acaatcaaca ccgccttcct ggaaaaaagc gccttccgcc ccaagacgga tcccggagaa
     2881 gaggaaataa aaaccttctg ggacaagcac aaggaaaatt acaaaaatga ggaagctcgc
     2941 ttcttcaccg tttacacctt cattcccgca ggcgatgccc aggcacccaa gcccggagac
     3001 atttccaacg ccaccatgga gaccatgaat ctggtggaaa atgacatctg ggaaccgctg
     3061 aacgccacca acggcaggaa catggaccag gccatcagcg aagccctggc caagactccc
     3121 ggcgtctgca aaatggagaa aaagtcctac acggccgtaa cgcgtaaaaa cgcgcctcag
     3181 gaaatcaacc aggcaatcaa ccaatccgct tccgacggac gcagcgccaa tctgctggat
     3241 gtggctttct ccctggcggg agccccggcc ctgagtgcgg atgcggatgc caaggccatt
     3301 gaagaagcgc gcgccaagac cggagccgaa cagatcagca ccatgcaggt gctggaaaac
     3361 ggacaagtcg tcctgatccg tctggaaggc attaccccgg tcaaagccct gccttacgag
     3421 accgcccgca acagcgcccg cgccgacctg ctggaaagca tgaccgttga atccatgaat
     3481 aaggccgcca cggaactcaa tacggaattg cagaaggcgg aaaaccccgt ggaacagttc
     3541 aatgccattg ccggaaaagc cggtgccaaa acttcctctt acggtccctt tgtcaatcca
     3601 aacgtactct tgagcagcgt caacctgcaa aacgccaaaa cacaggaaga gctccagtct
     3661 cttctggaac gggccatgaa ttcccgcctg gcccctccca aagagctccc ggcccctgaa
     3721 gaagtattcc aggcggcttc cgtcatcaac cccggccaga tggctcagcc catcaacacg
     3781 ggggacggta tcctgctgac ccagctcgtc aagcgcgaac tggaggatac gccggaattc
     3841 caaatcaaat ctacccagca aattgctccg gctctgacag cccaggcaaa atccatgatc
     3901 atgctggact ggctgaaagc gtgcatcacc caatataagg tggaaatcgc tcccatcctg
     3961 aaccaggaac gctaacgttt tccctttcat gacaaccgtc agtgagcccg tctccttctc
     4021 acaggccctg aacactgtct gaacgcattc gccatgatgg cctccgcccg gaggccatca
     4081 tttttattac tccatctccc tccttttcat tatcaaatga gcgccctgcc ctcccagtcc
     4141 agatacatca tcggaacgga agcctgtgaa cgtttcagct tctacggcat gaagtccatc
     4201 ctcatgctgt acatgacggg ccatctgctg atgagcgaca actgggccac ctccaccctt
     4261 catatttttg tgggaatggt ttatctgctt cccctggcgg gggcctggct ggcggacaag
     4321 gtctggggcc gatataaaac cattctttat atttccctgc tttactgcgt aggacacgga
     4381 gttctggcga cggcggatct ttttcacacc attgaagcgc gccgttacat tctcatggcg
     4441 ggcctgttca tcatcgcgct gggagccgga ggcatcaagc catgcgtctc cgcctttatg
     4501 ggagaccaga ttcccaataa gtccccgcag ttaatgacca aggcgttcaa tgccttctac
     4561 tgggctatca accttggctc cttcttctcc ttcctggtca ttccggccat ggaacagcgt
     4621 tacggataca gttgggcgtt tgccgtcccg ggcctcttca tgggggttgc caccttcgtc
     4681 ttctggctgg gccgcaaaaa ataccacaaa acgcctccgg cccggaacag cgggcagcct
     4741 ggcttctgga aagtcctttt catcattctg ttccacggag gctggaaaaa cgcggaacag
     4801 cgctgcggaa cttctgccgt ggaagacacc cggcacatcc tgaaaatcct ctccatcttc
     4861 gcctttatca tcccattctg gtccattttc gaacagacgg cttcttcctg ggtatcccag
     4921 ggcagcagga tgattcctct ttccatcccg ctcccgggcg gttcctggtc catcgggccg
     4981 gcccaaatcc aggcggccaa tcccattttc gtcatggtgt tcatccccct catcaccgta
     5041 tttgtttatc ccagggcggc aacgctggca aggcccctgg tgcgcctcgg aacgggattg
     5101 gccctcagct ccgctacatt cctgattgtc gctttcctgc aataccggct ggaggaagga
     5161 acctccatgt ccatcgcatg gcagctgatt ccttactgcg tactcaccat ctctgagatc
     5221 ctggtcagca ccaccggcct ggaattcgcc tacacgcaag ccccggcgca tttaaaaagc
     5281 ctcatcacca gtttctggaa cctcactatc tttgcaggca acatgctggt ggccgcaatt
     5341 acttttttcc tgtccaacgg agaatcagcc aacgccattt ccacggaccg cttcatcctg
     5401 tacgccgtgc tcgccgccgt ggtggcggcc gcctactcct tccgggcgcg caggtacgga
     5461 aaaacggaat aaagaaagca ctaccgttcc cgggcgcgtg gccgggttta accatgtttt
     5521 gaaaaaacaa tcaggccgta accagcatgc gctcctccca ttccctccgg caggtttcct
     5581 tttgggaaaa tgccggaaga cacaaattac ataaggagga catgaccgga caaaaactcc
     5641 ttgaagcagg tcaggagata cggcagtcgt taagcagctc gtccatgtaa aaggtcattt
     5701 ccgccaaagc ttcacgatat tccgtttggg gaaggagcca cagaatttcg cgggccgcct
     5761 cgtttttctc cagagcaacc tgaatcgcct tgtccagagc ctctgcaaaa acgggattgt
     5821 cctgaaaggc ggagtaatcc accacttcac ggttttcaat ggcgtcccgc agagtggctt
     5881 ccacattcct gtcaccgcat tccagcaaat tgaaaatggg aagggtcagc ttgcccttgg
     5941 tggcgtccgt atgaagcgtc ttcccggcat cgtcttcatc tcccaccatg tccaggcagt
     6001 cgtcgtaaat ctggtaggcc acacccagca gatcccccag ctggtaaagg gattcctcca
     6061 cttccggggt tactccggaa atccagccag ccccccccgt agctgcggag aaaagggaag
     6121 cggttttttt ccggatgatt tcaaaatatt cctcgcgggt catgctcaga tcatacaggc
     6181 ggctggactg ggccacctcc ccctggcata cgttcctgac ggtattcgcc agcctgcggc
     6241 agaattccgt gctgccgaat tccgtaccca gcaccatggc gtgggaaaag aggacatccc
     6301 ccagcagtac ggcgaggctg ttgccccaga gggcattcgc cgtagcctcg tcccggcgtt
     6361 tgtcggcctc gtcaatcacg tcatcatgca caagggaagc cagatgcacc atttccagca
     6421 gagcacccag gcggatatgg gcaggcttga cgccgcccgt agcgccggca accagcagaa
     6481 ccagggccgg acggagcatt ttacccttgg actggcatac cgtttccatg taatcggcca
     6541 cttccgggtc aaaacatgat gattgcgctt caacaaactc ggaaacctgc ttcaattccg
     6601 gcttaaccag ttgaagataa tgcttctggg atcgtaattt cgaaaagaac ggaatggaca
     6661 tgatatagaa cgggggcggc gctggtcagc gccgcaggga ttgtggcata tttaaagttc
     6721 cctagcaatg tatttcacag ggcatctttt tcataaccgg acggcaatcc gccaaacccg
     6781 gtccgccagg aaccggcctc cctcccggga taaggctcat tttgaccttg agccaggagt
     6841 ttttccttct acactgggcc atcatgagag ccttggtcta tgattgcagc gcgggcatca
     6901 gcggagatat gaatctggcc gccctgattg atttgggagc ggaccgggaa aacctggaac
     6961 gggaactgtc caaattgcat gtgcacgggg agtggaagct ggaatgccgc cgggcgcagc
     7021 agcacggcat ccggggaaca cggattgacg tgctgacgga ggaagaaggg cactccggca
     7081 gcagccatgc gcaccatcac cggactatgg cggatatacg aaaacttatt gagacaagtg
     7141 ccctttccaa taccgtaaag cggacggccc tttctatttt caccctgctg gcggaggcgg
     7201 aagcccgcgt acacggcact acttctgaag aagtacattt ccatgaagtg ggcgcggtgg
     7261 attccatcat cgatatcgcg ggagctgcca tctgcctgga aatgctccgg gtggacgccg
     7321 tcttcaccgg accggtggag ctggggtctg gcaccgtaac ctgccagcac ggaacaatgc
     7381 ccgttcctgc gccggcaacg gctttgctgg cgaaacattt ccgggccacc ttgaatggaa
     7441 ccacccatga ggccactaca cccaccggag ccgcctacat tgccgccctg gcgcagcccg
     7501 tcccctctcc gctggcaggc cgtattaccg caaccggcta tggaatcggc caccgccagg
     7561 gacttcccgt accgaatatt ctgcgggtga tgctggtaga aacggaagaa gaggaaactt
     7621 ccccggaact gctgacggag ctgtgcgcca atatagacga tatgacgccg gaacagacag
     7681 cctacctggc ggaaaagctg atggaggcag gcgctctgga tacctggcag gagtccgtct
     7741 gcatgaagaa aggccgtctg gcagtcaagg tttgcgccct gtgccagccg gaacagacgg
     7801 accgcgtgcg ggaagtcttc ttccggcaca gcagcacgcc gggcatcagg cagcatggca
     7861 tgctccgcca tattctgcgc cgggaaagcg cccccatcca cactccccat ggaacggttc
     7921 atgtaaaaac ttctttcatg catggccgcc cccattaccg gaaagcggaa tttgaagatt
     7981 gcagaatcct ggcggaaaaa accgggctgc cgctgggaca atgccagctg atggggcttt
     8041 ttcccacacc ttcccatgac aacgacgcca cagactccgt ttgaacggct tcgcgccgtc
     8101 cttctcccca ggaaacgcat tgccgtcgcc ttgtccgggg ggctggacag cagcgtgttg
     8161 cttgccgccg ccgtccgtat cctgggagcg gatcactgcc tggcactgac tgcgcgaacg
     8221 ccctacgtga tggaggagga agtgcgggac agcacggcgt tgtgccgccg cctgggcgtc
     8281 cggcaggaaa aactggcgtt tcccatcttt cccgcccttg cgaataatcc tcccctgcgc
     8341 tgctacctgt gcaagcatgc cctgttttcc tccctggcag ccagagcggc tgagatgggg
     8401 ttccccctgc tggcggacgg ttccaatctt gacgacctgg acgattaccg gccaggccgc
     8461 aaagctttgc aggaactcgg catcccgagt ccctttctgg aagcttccat ggataagacg
     8521 gatatccgcc ggctggcctg ccggctgggt cttccggaat caatcagcgg caaaccggcc
     8581 tatgcgtgcc tgctgacccg cctggaacac aaccgccccg taacggaagt tctgctgaga
     8641 cgcgtggacg cagcggaatc tttcctgaga accctggggc tgaaaggctg ccgtgtccgt
     8701 gttcacggag acagcctggc ccgcattgaa ttgccggaaa aggaacgcag actgttctgg
     8761 gacggacagc ttgcctccac cgcagcacgc cggctgcggg aactgggctt ccggcacatc
     8821 accctggatc tggaaggata ttccagaggg agcatgaacg aaccctcatg aacgacatca
     8881 cctccattct gcatcagttt aaaaaaggcc tcctttctgc ggaggaagcg gcggaaaaaa
     8941 tcagagccgg caccgtatcc gctccttccc atacggacat tgattacagc cgcctggaac
     9001 gcaccgggtg cccggaagtc atttacggcg caggaaaaac gccggaccag attgaagaga
     9061 tagcccgcag cctgcttgcc gccggacaaa atgttctggc cacgcgtctg agcggggaag
     9121 ctctggacta cctgaaccgc gcctttccgg cggcggacat gcacccggaa gcccgtctca
     9181 tgcgcattgc tccgaatccc gttccgcgaa cggcgggctt cgtcggcatc gtcagcgcgg
     9241 ggacctcgga ccaatacgta gcggaggagg ccgccctgac ggcggaattt ctgggcagcc
     9301 gggtagtccg gtaccgggat tgcggcgtgg ccggcctgca ccgcctggtt tcccatctgg
     9361 actccatccg ccaagctact gcgctggtgg ccgtggcggg catggaagga gctctcccca
     9421 gcgtgctggc aggcctggta aaggttcctg tgatagccgt gcctaccagc gtgggctatg
     9481 gagccaattt ccgcggggta accaccctgc tcgccatgat gaattcctgc gccaacggtg
     9541 tttccgtcgt caacatagac aacggcttcg gggccggttt caacgcccac ctgatcaaca
     9601 gcctggcatc ccggcccagc tgagggccgc ctgtccatga cggaacctcc ggcatccgtc
     9661 atggacagat cctccgaata ggcgttaaat gttccggaaa catccctctc cattcccgtc
     9721 atgaccagat ttcccctgtt ccatgnnnnn nnnnnatatc caccgttcat aatattgact
     9781 ggccctcccg ttccggcctc tccggagcag cccaacgcgc ggagctgctg aacatcctga
     9841 atacctgcgc ccagttgaaa ctgaacgccg tgtttctcca agtgcgccca aatgccgacg
     9901 ccctgtaccg ctcttctctg gaaccgtgga gccaatggct ttccggcccg ggcgtcaatc
     9961 ctgggtatga tcctctggct ttcgccattc aggaggctca ccgccgcggc attgaactgc
    10021 acgcctggtt caacccattc cgggcgaagg ccaacgtcaa gcatgccgta ggccgcaacc
    10081 atatttccct cacgcgccct gatttgatga agcgaaacgg ctccgtgctg ctgatgaacc
    10141 ccagcgcctc cgcctcccgc gaccacgcgc tgaaggttat catggatgtc gtgcgccgct
    10201 acgacattga cggggtgcac ctggacgact atttctaccc ctatcccact cccggccgcg
    10261 cctggtctcc cgccagcttt ggagacggga aatccccctc ccagcgccgg ggctacattg
    10321 acgacttcgt gcaagacatg tacaaatccg tcaaatcttc caaaccctgg gtgcgggtgg
    10381 gcatcagccc gttcggcatc tggaggccgg gcgttcccgg cggcattgaa gcgggagtgg
    10441 acgcctacga gcatctggcg tgcaacgccc gcaagtggct ttcccgcgga tgggtggatt
    10501 acctggcccc ccagctttac tggcgctgca gcccggccaa gcagagcttt cccgcattga
    10561 tgcaatggtg ggccgcccag aactccagac gcccggtgtg gcccggcatt gccacggcac
    10621 gtatcatgag cagcgaagac ccgggccgtc ccgcctctga aatagcggcg caggtcaact
    10681 actcccgcag ccttgccaga accgcccccg gacaatgctt ctggagcatc aaatccatca
    10741 tgcggaatgc cggaggcatc cagaaatacc tgaacagatt gtacccctcc atggccgttc
    10801 cgcccgccat gccctggtgc ggaaccggca cacccggaca accgcagaat ttttatgtgg
    10861 cggacaatgg ttccaccgta accctctcct ggcagccgtc cggcaatccc tcccggaaat
    10921 gggccgtcca ggcgcgctac ggcagccagt gggctacgcg catccttctg ccgggcagcc
    10981 agacccgcgt aaccctgccc aaatccttcc tgggggatgc aggatctgtc gccgtcaggg
    11041 gcgtcagcgc ctatggagcc cagggcccgg cagcagccgc cagaaggtag aaaagctttt
    11101 tcccagctgc ctctacaaga agaaaaatag aacaccgagg ttcctatttt ttgaacaact
    11161 ccaaaacaat ccatgtccaa acacccatgc attcccatgg cgcggacatg aacagaaggc
    11221 gttttattgc tctttcctca ttggcggcgc tgggaacaat cagccaggcc cgggcaacag
    11281 ggccattcat acaaccacag cacaagatga ctaaacagca agccccctcc acgatcggct
    11341 atgccgacgg caaatatgta ctgccacccc ttccgtacgc ctacgacgcg cttgaaccca
    11401 tgctggatga aaaaaccgtg cgcatccacc acgacaagca tcatgcggcc tatgtcgccg
    11461 gagccaatgc cgcggctgaa aagctccgtg aaattgcgga cgggaaactg gacgcttccg
    11521 ccaccaccaa ctgggtgcgt aacttgtcct tcaatgcttc cggccatgtt ctccatacca
    11581 tttactggac caacatgacg cctgatccca aaaaagagcc gcaaggtcct ctggtagatg
    11641 ccatcaagga aaaattcggt tctctggaag ctatgatgaa ggaattcaag gcggcttccc
    11701 agggcgtgga aggttccggc tggggcattc tgggcgtgga ccccatcagc aagacgctgg
    11761 taatctgcgg ggcggaaaaa catcagaatc tggaaattcc cggtctcgtc cccattctcg
    11821 tctgtgacgt ttgggaacac gcctattatc tgaagcacca gaacctccgg gcggactata
    11881 ttgaagactt ctgccgcctg atcaactggg acgatgtgga acgccgctac caggaggcta
    11941 tggcttcctg accgtttttg cagaagttga tttttcctat ccccaaacgg cacgggcttt
    12001 ctgtagtccg cgccgttttt taacgcattt tcatctcgct cttgcatccg gcacggcaat
    12061 agggcatact gttgcagact aattatctat cattacacgc atgaaaaaag ttttcgtctc
    12121 cggctgctat gatatcgtcc acgctggcca tatccagttc tttgaagaag cccgcgctct
    12181 gggcgactat ctaatcgtct ctttcgcttc ggaacccgtg ctgtggcacc acaagcagcg
    12241 caagccgtcc attccggacg aacacaaaaa agttctgctg gaaagcctgc gcatggttga
    12301 caaggttatc ctgggtaccg gcatgaaaaa gggcctggat tttgaagaag agtttcttca
    12361 ggaaaaaccg gacatcctgg ctgtgacgga agacgacctt tacagcgata tcaagaagga
    12421 gctgtgcgcc cgcgtcggag ccaattacgt ggtgctcccc aagacgcccc ccaaattcac
    12481 tcccgtctcc accaccatgc tggtgaaccg catcaaggct ccgtccgccg taccgcttcg
    12541 cgtggacttt gccgggggat ggctggacgt gccccgctac gccagaaaag gctcctatgt
    12601 ggtcaactgc gccatcaccc ccatggtttc cctctgcgaa tggccgtatg aaaaacgctc
    12661 cgggctggga ggcagcggcg cgtgggccat gctggaaggg cgcgatccgg tcgcgtccga
    12721 actcgctctg ggcgtcggtt ggcaggatcc tgccgtcatt gcggaaacgg ggttgtgcgt
    12781 atggcgctcc ggcagttctc cggtgctgga tgtcaaaggc acgggtgact tcctggaagg
    12841 aagaatggcc attctatata cgggtgaaga acacgacacc ccaaagatgg cggatgaaca
    12901 acgggactac gtacgcatct cccaatcctc cctcattgcc cgcaccggag ttctggaacg
    12961 caacatcaat acactggcgg caggcgtagc cctgtactac agcgtgcagc ttgacgaagg
    13021 aatgcgcccc ctgccggata ttcccaatgc ccttgcaaaa aaatatctgg gaggcggcta
    13081 cgggggatac gccctgtacc ttttcccctg ccgggatgat cgcgaccagg ctgtcaagga
    13141 caacccggca atgaaacggg tggagccgta ttgccgccag ctgttcaagt aaattccccc
    13201 cctttcatgt ccctgatgcg gaacagcctg gttgcctccg gcgccatctt cgcctgccgc
    13261 ctgacaggca tggccaggga aattgtgtac acctccctgt tcggggccac gggagccctg
    13321 gacgcctttt acacggcttt ccgtattccc aacctgttaa gagatctgtt tgcggaaggg
    13381 gctctttccc aatcctatac cagcgtagcc tccaaaacgc gggaggcgca gggagacgcg
    13441 gccgcgtggg aactgaccaa caaggtagct acccagcttt ccgccctgat gatcgccatt
    13501 gtaacgctgg ggattctatt tgcagggccc gtcatggaag ccctctacag cggggatcac
    13561 tctctggcgg agcaattgtt cgccacagac ctgagccgca tcatgtggcc tttcatcgga
    13621 ttcgcctccc tgtccgccct catcatgggg gcgctgaaca tggtgggggt tttcggattg
    13681 cccatgctgg cctccgccgc tttcaacgtt acttccatcc tgctcggcct gctgattgga
    13741 tatttcattg atccatcctt cggccccaaa gctttgtacg gttttgcctg cggcgtcacc
    13801 atcgggggca tggcccagat tgccgtgcag ctccccaaac tcagcaaaac cggcttctgc
    13861 tggaaaccca atttccaatg gaacgatccc cgcgtgcgca aaatctgggg gctgatgctt
    13921 ccctccgtgc tggcttcagg cgtgacgcag ttcaccatct tcatcaatac ggggttcgct
    13981 ctggatctcc aaaaaggctc cgtcaccgcc ctgaccacgg cgttccgcct ctggcagctt
    14041 cccgtggggc tcttcggagt tgcgacgggc atggtggtgc tgcccgctgt ttcccgcatg
    14101 atggtgggag acggcagaaa agaggtggct gtccacatcg ccaaggggtt gcggctggtg
    14161 gcattctttg cggtacccgc ttttctcatc ctctccatcc tgggaacgga attcgtctcc
    14221 tccgtatacc agtggggccg cttcaaccag gaggccgtgc gttacacggg ggaagtgctg
    14281 ggagcctatt ccctgggcct gctgggctat gccgggacca aggtggtaca gcccgtcttc
    14341 ctggctcttg aaaaacgctg ggttcccctg attgccgccg ctgtggctct ggctatcagc
    14401 atcggcctca actactgttt cgtctacatc ctgcataaaa acgcggcgtg gctggcgctg
    14461 accacctccg tggtcactac cttcaatttt ctcttttact tcttttacct gcgacgccag
    14521 ctgggcggcg tagacggacg gacgctgctt tccggactgg ccaggatcat ggcggcggga
    14581 atatttctgg gggccgtgtg ctgggcaggc aaggcatggt tcctccaggg cttcctgaac
    14641 tggaccttcc cggccagggt gctggggata tccctagtct gcggttgcgc cggcatcgtt
    14701 tacctggcgg cggctttcct gctgaagacg ccggagctgg aagcggtacg cgccaaattc
    14761 ctgaaacgct gacgggaaaa atcccttcca tagagggagg aacgccctcc gcaagaaaaa
    14821 gaatggccgc cgcaagcatt ctccttgcca ggggtgcgct caatgagtaa agtttttccg
    14881 cctatgcgga gaacagcctg ttttccgcca gggcatcaaa atctacgact gcaccacaac
    14941 aatggacatg atttccccca gcattgcggc attgacccct tccctgaccc tcaaggtgac
    15001 caaccgggcc aaggctatga aagcctccgg tgaagaagtt tacggtttgg caggcggtga
    15061 acctgaaatg gacaccccgg aaaacatcaa gcaggcagcc atcgcggccc tgaacaacgg
    15121 agccaccaaa tatacgcctt ccgccggtct tcccgcactc cgccaggcca tcgcagaaaa
    15181 gctcaagagg gaaaacaacc tggaatacga tttcacccag atcaccgtgg gagccggcgc
    15241 caagcacgcc tgtttcaacg ccatcatggc gaccgtatcc gagggggatg aagtcatcat
    15301 ccccgccccg tactgggtca gctatccgga aatggtccgc atgtgcggag gcatccccgt
    15361 catcgtggaa accacggcgg aaaacggatg gaagcttacc gccgaacaat ttgaggaagc
    15421 catgaccccc cgcaccaaga tggtcatcct cactaccccg aacaatccga ccgggtccgt
    15481 ttattccgaa gaagaactgc gcgccctggg agaagtggcc ctgagcgaag acatcctcat
    15541 cctggctgat gaaatttacg aacacctcgt atacggggac accaagcacg tcagcatcgc
    15601 ctccctcagt ccggaactgt acgacctgac catcaccatc aacggcttct ccaagggcta
    15661 cgccatgacc ggatggcgcc tgggctattc cgcagctccg gcagccattg ccaaggccat
    15721 ccagatgatt caggatcaca ccacttccaa cgtctgcacg ttcgcccagt atggaggcat
    15781 tgaggccctt acgggagacc agagcttcat cagcgacatg cgcgacgaat atgacatgcg
    15841 ccgccagtac gtttactccc gcctcagcgc catcccgaat atcagcgttg tggagccgca
    15901 aggcgctttc tacttctttg tggacacaag caggttggga ctcacctccc tgaatctgtg
    15961 cgacaagctt ctggaacgct acaaggtcgc ggccgtcccc ggcatcgcct tcggcaatga
    16021 caaggccatc cgcatcagct actgcaccac gctggacatt ctgaaagaag ctctggaccg
    16081 ctttgaagaa ttctgcaagg cgcattaaaa aacgttgatc accataaaaa ggcgttccac
    16141 gtcaagcgga acgccttttt attcttccgg atttccctcc tgcagcaaac ctgcggcaag
    16201 gaaatgcgcg ccatgcctcg caaccggatt gccctgcacg aaaaggcatg attaaataaa
    16261 tctcatatga atacccccgt tctcaacgac aacctgcgcg ccgccacgga agccctttgc
    16321 aatctgctcg ccaaggaaga caaggttgtg gcctccaagg caaaaatagg tctctttttc
    16381 caaaatccgg aagcaaccaa gctttttgaa gaagtaaacg cttacggaga agaactgcgc
    16441 aacaagcacc tggcgggaat gcctcctacc gaagaggaaa tttccaaatt cgacgccctg
    16501 cgtgaaaacg tcatcaagaa tgatgcggcc cgcggctttt tggaagcccg ccagaccatt
    16561 gacgaactcc tgaacaccat caatcattat ctgggcatgt ccattgacct tggccgcgct
    16621 cccactcctg aagaaataga ggaagcccgc cagcgcgcca tgagcgccca gtcctcctgc
    16681 tcctgcggcg gccagtgcga caaggaaaat tgcgattgcg acggcaagtg tgaccacgac
    16741 cataaggacg gcggttgcgg ctgcggcaac cattaagaca atctttcaac ttttgccaaa
    16801 cggcttcact caaaaagtga agccgttttt tcttcccgaa aacaaacgat actgtccagc
    16861 gcgcctgcaa tttcctccgg aaaaccgggc ggactactgc acctctttat tgagcgccat
    16921 attttcgacg ctccgatgtt tgcacagcac cggcatcttg caccttccct tcttcgccag
    16981 aacaggcaag atgcttgaat gggacggaaa cccgtgctag cctcatccta aggaatcatg
    17041 actttttcca accgtcccgc tcccctcccg ttcttcaaaa cggatttccc gctgttcctg
    17101 gctccaatgg ctggagtgac ggaccccatt ttccgcacta tctgcaagga gcttggagtg
    17161 gatgtaatgg taacggaatt cgtctctgcg gaaggaattc tccaggcatg ggaaagaaac
    17221 cgcaggtaca caaagataga aaatgcccac cgccccatag gcatccagct cttcggagcg
    17281 gatgggaacc acatgggaga ggcggccaga attatcctgg accatgaaaa gccggatttt
    17341 atcgatatca acttcggctg cccggttccc aaggtggttg gcaaaaatgg aggttcctcc
    17401 ctcctgaaag accttcccct gctggcttcc gttgccggag gcgtcgggaa agccgtagga
    17461 gagcgaagcc ccgtcacggc caacatacgt cgtggcgggg agtttcagag cctgtgcgcc
    17521 atggacgcgt gccgcctgct ggagaatgag ggaatttcca tgatcaccat tcacgggcgc
    17581 acccgctccc agcagtattc cggaacggcc aactgggaca ttattgacga atgcgcgcgg
    17641 gccatgtccg tgccggtcat cgggaacgga gatatttcca ccccgcaggc cgtagcttat
    17701 gtaaaagaaa atacggccgt cagcggcgtc atgattggcc gtgcggccat ggaaaaccca
    17761 tggattttcg gagacgccaa gtattatctg ctgcacggaa ccatgccgcc ggccagagac
    17821 ccgcaggaaa aggcggccct gattttgcgg catgcccgga tggccctgga atcccgccat
    17881 tatggggacg aacttcacac catgcgccat atgaggtcac gaatcctggc ttatacgaag
    17941 ggatttcccg gcgccaagga aatgcgttcc cgcctggtgc gggtatccag catgccggag
    18001 ctggaagacc tgctttccaa gctccccagc cagaagccct gagtacaggg aaaatcccgc
    18061 tttccggaag attccgcagg gtttgaaaag cggcggagag ggaaggaggt tcttcccgcc
    18121 tccggacacg cccgaaaaaa aatgccattt tgagggaagc taagttgact ttacgttccg
    18181 aacctgtcag aaatagggga gtatcctgcc gctccatttc ggcagacttc caacaatagc
    18241 atccagagat cgacgtgtac tccaacgaat catatctcat cgaacttctt acgcaggccg
    18301 gatatctgaa tgaagaaatt cttcagtacg cgcgcagtca aaaatctccc acccaggacc
    18361 tggtcgattt tctgattcag gccaattatc ttacggaaga cgtcatcgct caggtggcgg
    18421 cgtcaaactc cctgctcccc gtcgtggacc tgggctccat gcacatcccc caggaggtac
    18481 gcgaactcat tacgccggaa cttgccaggc gcttccgggc catccccatt tccgacgacg
    18541 gtttttccat caacattgcc attgacgacc ccctgaatct ggaaaccatg gacagcctgc
    18601 cccagctgat ggggcgggat gtgattttca gcgtcgccac ccgcagcgcg gtagaaagcc
    18661 ggttgaatga attttaccgg gatctgagcg ttccggaaga aaccgacggg ttggaagggg
    18721 aagatgcccc cattatccgg ctggtgcagc agatgctgac ggacgctttc aaaatgaggg
    18781 cttccgacat ccacattgag cccatggaaa acaggctccg catccgctac cgcgtggacg
    18841 gcaagctggt ggaagtggcc acgcatccca aaaaactgct cagccccatc attgctcgcc
    18901 tcaaggtaat gagtaccacc atgagcattg cggaaaaacg catgccccag gacgggcgaa
    18961 tccagatgag catcggcggc aagcaaatcg acctccgtgt ttcatccgtc cccagcaacc
    19021 acggggaaag catcgtcatg cgtatcctgg acaaatccgc cctggtgctg ggccttcccc
    19081 agctcggatt tttctcggat gatgaagctg tgttcgaccg cctcatcacg ctgccggacg
    19141 gcattatcct ggtgacgggt cctaccggtt ccggtaaaac cacgaccctt tacgcgtgcc
    19201 tgaaccacat caaccgcccg gataaaaaaa tcatcacggt ggaagaccct gtggaatacg
    19261 aactctccgg catcaaccag gtaatggtaa aggcggatat cggcatgacc ttcgccgccg
    19321 ccctgcgcgc catgctccgc caggctccca acatcatcat gatcggggaa attcgagaca
    19381 tggaaacagc cagcatcgcc atcaacgcct ccctgacggg gcacctcgta ttctccaccc
    19441 ttcacaccaa tgacgctccc agcgccgtgg ctcgtctggc ggacatcggc atcaaacgct
    19501 tcctgatcgc ctcctccgtc cgcgccatca tggcccagcg tcttgtccgc aagctgtgcg
    19561 accgctgcaa ggtggacggc actctgacgg aaaagcaggc gcatacgctg aacattgaca
    19621 tgtcccgcct tgcccagggc caaatcaagg cgccccacgg ctgcgacttt tgccgcggcg
    19681 gcggattcaa gggccggatg gggctgtttg agattttcga aatcgacgac gaggtgcgcc
    19741 gcatgattaa cgaaaatctg acttcccccc agctgcgcca gcgcgcccgg gaactgggca
    19801 tgagaacctt gagggaagac ggcgtacgca aagtgctggc cggccttact tctccggaag
    19861 aagtgctgaa cgtcaccatg ggagacgcca actgattttt ccccgctatt cattctgctt
    19921 attatggaca ccaacctcac actggaactt ttcatcggcc ggggaatgat tgacaaatcc
    19981 ctggcaaagg acatcaagga ggaaatgatc gcctccggca aggaactgcc ggaagtgctt
    20041 gcagacttcg gcatcatcgg cagcaaggat gatatctggc agatgattgc cagcgacctg
    20101 ggtacggaat tcattacact ggacaacttc cagccggacc cgaacgtgca gaacatgatg
    20161 ccggccacgc tcgtgcgcct gcacggggcg ctccctgtgc ggcatggtcc ggaaggcctg
    20221 tacgtctg
//
LOCUS       DAWEMX010000035        20191 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1113_length_20191_cov_11.413240, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000035 DAWEMX010000000
VERSION     DAWEMX010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 20191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20191
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_20191_cov_11.413240"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6029..6128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19500..19509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4102 a         5148 c         6245 g         4586 t
ORIGIN      
        1 gggaagggat ggtaatctat aaaaaaagaa ccgccccttc acggagacgg ttctttttga
       61 taagaaagca aggagcggat atttcgtccg catgggccgg attttagccg agcaccttgt
      121 aatactgaag gtgaatctgg ggattcaggc gaaggcgttc gcggatgggc ctgatggcgg
      181 aaggttccgc agagaacgtg taggtcacat actgcccggc ggaaaggtgg ttggattcgt
      241 aggcgaattc gcggcggccg gcgttttccg tggcggtgat gtcggcgcct tcttccttca
      301 tcgcggcggc tacggtgctg atcaactcct cgacgggagt ttccgtaccc ttcatgttga
      361 atacgataag agcttcgtaa tttctcatgg tcttgtgata attagaaatg tgtcgtgcga
      421 acggtgcgca ccttgcgggc aagtaaggtg caatgattgc cttcagaaag caagcctatt
      481 ttttaccttg tttccgggaa catgccgttt cctgcgtttt actctcccca cggcgcggaa
      541 gaattgttct gcatggcgtt tcctgccgtt gtccttagcg gaggagggcg ggatttccgg
      601 tgaggaatcc ggaaaagcgg cagatagagg cggcggagag tttttccggg ctttctgtcc
      661 tgccgcctga acaatggtgc cggttccaaa aaaggatcat gacaaggagg ccttggaact
      721 ttacgccgca gatgaattaa cgatgcgtta ttttttggaa tcaggaagaa ggagaaaggg
      781 cttctccctg atggggtgat attccggtac tgggattcac ccccggaacg ggatggtgca
      841 ggtatggcgg gaaaaattcc ggaaagaaga aaaacgttaa cgcctccgcg ccatctgtac
      901 ggccagctgg aaggcgtgga tggtgctgga cggattggct tttccggttc ccgccaggtt
      961 gaaggcggta ccatgatccg ggctcagccg ggggcgggga aggcccaggg tgacgttgac
     1021 ggcggtatgg aagtccacca gcttcagcgg aatcaacccc tggtcatggt aaggggccag
     1081 cacggcgtca tagtttcctt ccacggcatc ccggtacacg cagtccggga cggaaggccc
     1141 gtcaaatgca gcatccggat ggatcgccct cagccgttcc acggccgggg caatgatgtt
     1201 cccgtcctca tcgccgaaag cgccgtgttc cccggcgtgg gggttcaggg ctgccagggc
     1261 gatacgcggg cgcatgattc ctttgcggat gcagaagtct ttcagcaggg ttccgatgcg
     1321 gaccagttcc tctgtttcca gcagggaagg cacgctttgg agggaagtat gaatggttgc
     1381 cagccccacc gtcaaccgtt tcccggtcag gcacatggcg taattgtccg ttttcaggcg
     1441 ttctgcaaag aattccgtct gccccggcca gcggaaacct gcctcatgca gggtttcctt
     1501 gcagacgggg gcggtgacaa cagcgtcaac cgttccttcc cgcagggcat gggccgcctg
     1561 ttccagatgg tcgaaggcgt gtttggcgga ttccgcatta ggccttcccg ggggaagctc
     1621 cactctcttg ccgatgagga ggtactctgc gtcttccggg agttctccgg aggcaagcgc
     1681 ggcggagatg acttccgggc ctatgcccga ctggtcgccc agcgtgtatc caatgaccgg
     1741 tttcatagtg cggcaacagg agggattaca attccgcggg ttccggcatt ccggcagggg
     1801 aagcgggaat ttcttccgcg ggggaaacag gagttttttc tgccggaaca gcctgcaggg
     1861 ctgccgcccg tttttccatg ttgatgacct gtgtcgtttt ttccggttcg tcggggttgc
     1921 ggagttcgaa ttccaggtgg gagttggtgg accaatggta ataaatggtg gcgccgaggg
     1981 ctgccagcac cagaatcagg agaatggaac cgaagaagga gcgcatgcct ctactgtgtc
     2041 ccggaggagg ggggaattca agaaattatc aagaccgcct gcgtgggcgg gcaaacgccg
     2101 ttaactgccg tgtatcttca tttcctggaa gaggaaagaa gggtaagtaa aaagaggcag
     2161 cccggcatga gaagatccgg gacagttcag gacaggattt tcatcgcgag gaacaggagc
     2221 atgagcagga tggcaaagcc gatgattctg cccgccatgc tgccgtcttt cccgtcctgg
     2281 atgcacatgg ggcagttttc cgggccgttc atgccgctgc atcccatgcc gcacgcgccg
     2341 cagggaaaat ccgcctcccg ctcctgtact cggtcttgga gaaggagttc cagctcgcgc
     2401 acggaaggct ccacgccgta gacgacgatt tccccgtcaa tggcgagtgc gggaagcggc
     2461 gtcccaattt gttccggaac ggtctcttca gcctgcacct ccggattgac gcccagatgg
     2521 gccaatgcct ggagggtatt ggcacgcagg gcgtttcccg ggtaggcccg gctgtaaaaa
     2581 agctgaatcc tggtcatgga actgggtgtg tcaggaacgc ttccgtttgg gaagactctt
     2641 cttccaacaa ataggacggg gctccaaagg tcaatgcgtt tttccgtgtc atgacatata
     2701 agtgttgtac ggaaaacatt ttagactttt caaatgagga gggaacgggc actatcccgc
     2761 cggtcccgcc atgaaaaagc tatctctatt ccttttgacc ctggcctgtg tattccagtt
     2821 ttccgcccag gcacaggaac ggcccagtta cattgccatt gaggccaatt ccggcaagct
     2881 tcttttcagt tccaacgcag aagaaaaaag gcccgtggcc agtctttccc aggtagccac
     2941 cgccatggtg gctttggact gggtggcccg cacgcggttg ccgctggaca ctttcattgc
     3001 cgtgccccag gaagcgttcg gcctccgtgc gggcaatccc atggatctcc agccgggaga
     3061 ccgcctgacg ctccgggacg ctttgtattc caccctgctg ggttcggaca atgtttccgc
     3121 cctgactgtt gcctcctttg tggggcgtga cctggtgtcc cgccggggcg gcggcgcccc
     3181 cattgccgct tttgtggcgg aaatgaacaa tttggcccgt tccctgagga tggccaaaac
     3241 gcgctttacc gctccccacg ggctggacgc cggcaactcc gtttccactt cctgcgcgct
     3301 ggatatggcc ttgttgggcg tttacagcat gcaaaatccg gctttttgct acattgtttc
     3361 ccaggcgagc cggcgcattg aagtgcagtc ccagaccaag ggcaggacga tgtacgatat
     3421 ttccaacaac aacaggctca tgagcgcttc cggcgtggac ggcatcaagg cgggcacttc
     3481 ccgcgccgcc ggcccctgcc tgctgctcag cgtgacgcgc aacgctattt cccgccggag
     3541 cccccaaacg gggacggagg tgatttatcc gcagcgcatg attattgcgg tgcttggttc
     3601 tgcgagccgc tattccctcg ccagggagat gatgaacacc ggctggcgcg tttgggaaaa
     3661 ctggcaggct tccggcatgg atatgaagga ccccaaggaa tttgtccagc ttcctgtgaa
     3721 atctgctcca caaaggagat aggaagtaga ctgggaagga tttttctgct ataaggaacg
     3781 ccgaatatga acatcggtgt tttgaatagc gggggtgact cccccgggtt gaatgcagtt
     3841 attgaaggtg ttgtgggcgc ggcttcccgt cgcggctgga acgttgtggg tttttacgac
     3901 ggttttgaag ggcttttgtc cgaggaaggg gacgaacggt ttgaatggct gacccctgcc
     3961 gcctgccgcg gattgcgagc caaggggggc accattctgg gaaccgtgaa caaaggaaac
     4021 ttcgccatca aagtaggcgt ggaccaaaag ggggcgattg aacccgcggt gctggaaaaa
     4081 acgaaggcta ctgtccgccg gctggaactg gatgccctga tcgttgtggg cggagacggc
     4141 tcccagtcca cggctctttt gctttccgag atagggctcc ccgtagttgg cgtgccaaaa
     4201 accattgata atgacctggg cgcaaccgat gtcacttttg gcttctacag cgccgtttcc
     4261 attgtttccg agtctttgga ccgtctggaa accactgcaa acgcgcacca gcgcatgatg
     4321 gtggtggaag tgatgggacg gcatgcggga tggattgcct tggaaggcgg catcgcaggg
     4381 agtgctgatg ttattcttct gccggaaatt cctttttccc ttgaaaacgt ggtggaatgc
     4441 atcaaggcgc gcaaggctgc cgggcagcgg gaaatcctgg tggtggtatc tgaaggggcc
     4501 cgtctggcgg acgaactggt attgctggat gaaaagaccc agggggaagt tcgcctgggt
     4561 ggcatcggca aggtgattgc caagaaactg gaagaagcca ctgggattga aacccgctcc
     4621 tgcgtgctgg gccatatcca gcgcggcggt tctccctgtt cctatgaccg gattctggga
     4681 acgcggttcg gcagttatgc cgtggaactg gtggaaaagc gccaattcag ctgcatggtt
     4741 gctctccgtg ggacgcagat gactgccgta cccattgagg aagctgtgaa aaccttgaag
     4801 ctggtggatc ccgattgtca gctggtgcgc acggcccgcg atttgggcgt atgcttcggg
     4861 gactgacgtt ctccgtctgc gtcacgggaa attgattgta cccgataggg ttttgattgc
     4921 ttttcaaccg tatcctggaa tcaggatacg gttatttttt gccgaagcac gccgtattca
     4981 ttggggcctg gcgagctttt aagaagcatc atggacagga agaggatgga aagcagatcc
     5041 atcaggctgg acaccattcc caggtagtcc agataggggg ccatgtcctc cgggaccagg
     5101 cgcagcagga aaatcagcag gaaagtgacg atgaaaataa gaatgggaat caggatgaat
     5161 tttccgctca gattgctgtc atgcaggcgg cgcacggcga ccggcagatt gaagaggata
     5221 ttgagccccg ccacggctgc cagaagctcc agggaaagca tgctcagctc tccggattgg
     5281 tagaaaaagt aaaaggtggg attgaagcag gagtgagcca gcaggatcag agcatcttgc
     5341 ggatgggcgg catataacag gatttggagg gaaggagaga cataaaaacc cgccggctgg
     5401 ctgtcaacgt ctatcatgct gtagcccagc ggaaggaaca ggaggacgga aaggaggaaa
     5461 aaggaccaga attccgtccg ggacgcgcgg cctgccaccg tattcaattt ccgcaggctg
     5521 atgagccagt aaaggtaaaa cggagtcaat ggctgctctt tcagttctcc ggcaggggga
     5581 tcagccggtt ggggctccgg acggcggctg tatttgttgc cgtccgcctt gctttctcct
     5641 agcgtcagat aaaaaaagat cagatgcccc agcccgaagg gaatgaaaag ggaaaaaagc
     5701 cagaggccgc tcctgcctgt atcatgcagg cgtctgaacg cggctgatat gcttgggatg
     5761 aataacaccg cccacaggag gatggttaag gcgtctccgt agatgctcag tcttgtatca
     5821 gggatgtgca gccagtccgt caggatatac cccgccaggg ctggaatcag cgccaggagg
     5881 ggaagagcca tgaaccacca gaattccgca cgggaagcgc agccccgaaa gtccgcataa
     5941 tgaagaaaac cttttttcca gcaggagagg ggggaaagga gggaagatgc ctgcgtggag
     6001 gttggaggaa cggaggaaac agcaggagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnaa gcaggagaag caggagaagc aggagaagca ggagaagcag gagaagcagg
     6181 agaagcagga gattccggca tggatgaaaa gggggtttcc gtttagactc cctgatgccg
     6241 gggaatgctc aatgcgtctg tgcccaggac atggaaagtt ccctcctgca gccggttcag
     6301 atcaggacaa tcgccatcag cagcattccg gcgatgatgc ccatgatgga aatgtgatgg
     6361 tgtccccagc gttccgccat gggcagcagc tcgtcaaagg agatgaagac catgattccc
     6421 gcgacggagg caaagagaac cgcaagtacg gtaggtgtga ggaagtggaa caggaagaac
     6481 atggcgatgg ccgcgcccac cggttccgcc agaccggaga gaaaggaata aaaaagggcc
     6541 tttttacggc tgcccgtacc gtagtaaaga ggtaccgcca cggctattcc ttccggaatg
     6601 ttatggacgg ctacggccag ggcgatggag gtgcctaaag tcagggagtc caatccggcg
     6661 gcaaaggtgg cgattccttc cgggaaattg tggatgccga tggccagggc gaacagaatg
     6721 ccggaccttt taattcggga tgaggaaaat tcaccgctga cctgtccatg gatgtcttcc
     6781 ggtccgcgcg cttcgtgcgg gttttcgtct tccggaacca ggtaatcaat cagagccgcc
     6841 acggcaattc ccccgaagaa ggaagcaatg gcgatccagg aacctgccgt atggccttcg
     6901 aactccatca acctgtgctg ggcttctccc agcagttcca ccagggagat atacaccatg
     6961 actccgccgg ataatcccag ggcaaaagta agtgcctttg tatccgtcct tttcatgaag
     7021 aaggcgatga agcctcctat gccggtagca agaccagcca gcgtggtgag caggaagaca
     7081 acgaggatgt ggtcggcgga aaggtccatg cgtgcacctg ccgggcaggg atgaccccgg
     7141 cagtaaagaa aggggtgtat tattttgcgg gagccgtggg aacgatcatc ttcgtggccc
     7201 ggatggcgcc ggggggaagg gggggctctt cccccggctt caggaggccc cgttccttct
     7261 tgatttcagg cagcttgtcc agaagtttgc cgaacgcttt tccttcaaaa ctttttggga
     7321 ggagcgcaac gccttctttg agaggctgtt ccaattcatt gaccctgttt tgctgcatga
     7381 gcaggtacac tttttgcatg agaacagctt gtttgacttc cggaagaagc ccgttctttt
     7441 tcaagtagct gtccatgatg gaaagcgctt catcaggatt ttgatccgcc agaatgtgga
     7501 tgtttttctt ccggaacgta tcggcccatt ccaatttctg ggcatggagc agatcgtccc
     7561 tgtgcagttt ggcgagaatt ccggaagcgt cctgcgggtc cagtttttcc agttctgcca
     7621 gagccggctt atagaaagtg cggatgctgg attggggaac cgttttcagc aattgaacca
     7681 gcgtgtcccg ccgctgctgg tcagtgagct tttccgccag cttgtttgtc agggactgct
     7741 gcgtgttgag tgcttccgcc gctttttttg tatcctgcat gacctgggca ggattggcgt
     7801 aacctacgac ggtgtggacg ggccgcccct gggcatccgc aaacaccagc gtggggaatc
     7861 cggtgatgcc gtaggatttt ttataggttt ccagagaagt ttttgtctgc gcgtcctgct
     7921 gctttttacg tgggtagtcc agttccacgg gaataaagga acgggaaatg gctgtctgga
     7981 tttcaggaag ggaaagagcc tgctttttct gcatgatgca ggctccgcac cagtcggaac
     8041 cggtgaattc cagcattact cccttattct gtgcggcggc ttgctgcatg gcttccgcag
     8101 ggctggtact ccatgtttcg gaggcgccgg cactccaggc gaggagtatg gctgcgaggg
     8161 gatgtatgat tgttttcatg gcaggaatgt cattattgca tggggggaag cggagaatgg
     8221 gcggggggaa cccacatgtt tccggaagag gagctgccgg aatctccggt atgggtaggt
     8281 gtttcaattt tcggttttcc ggcgggcggg tccagatctt ccaccaccac tttctttttg
     8341 gcgggctggg gcacgggggc ggcagccggg gccggtgagg gcgccctgcg gataacgggg
     8401 gatttgaggg aagcatccgt ctggttcatg gccgtaatat tcagcgggtc gccgtaggcc
     8461 agcatttccc ggatgtcgtc aggaagatct ccggcaggaa tggaggtgct tccctgttcg
     8521 tgcatgatac gcacgaattc attttccacg cgggtgattt ccacattcaa ataagttttg
     8581 cctgaatggg atgtcttcag aatgtcgaac ttgcggccgc gtgccttggc tctggcgtgt
     8641 tggcggtagc gttcgaagta ggagcggatt tctccccgga tgccggctac gcgcattttc
     8701 tctttctcaa tggatttttg cgtctcttcc aggtcggagg ccgtatgttc aaagatatcc
     8761 aaaaggctga gcagctgttc cttgggagcg gtcctgttcc gtatttccct gagctgttcc
     8821 tgcttcttca gcagctgggc atgattgtcc tccaaagctt cccgagcatc tttgacaggg
     8881 tctggaagct gggaaaattt ttcataaaca atatagccgg cactgatgac gataagggcg
     8941 aacagcccca ggcagaggtt ccagaaaaga gagtctcccc tggctttccg ttcggaatcc
     9001 tcctgccggg cggattcttc ttccgcagcg tgttccgaga cgacgtccag ggaggttttc
     9061 agacgttttt tggctgcggg ggcggagttt tcatccttat attctcctcc gttctccttc
     9121 ggagcaaagc gggtttcgtc ttccgcaggg gggagagttt cgccggacgg ctgtctggga
     9181 gcgggggcgt cggtcatggg atgcgcggga atggaggtta gtctaccggg tgggtttgtt
     9241 tgaattcagc cagtttggcc gcatacgcgt tatattgata ttgcagggca tggaattcct
     9301 gcttgcgctg cgccagcgtt ttgcgcatgt cttcaatgtc aaagcacagg tgctcgaagt
     9361 cgcggcgttt ggaaggcagg acatctatat ccttcaccgt gaagggcgtc gtgattcttc
     9421 ccgggttgga aagccttttc tgaagattgt aattaatgcg ggcatcgtgg gattccgcct
     9481 ggcttatttc ctgttccgtc tgggccagtg cgtttttcat ttgctggagc tgggcctgga
     9541 gctgctggtg ttcctgggat gaattccccg tcaggaagga ggtcagggct tcattcgtac
     9601 tgctgtcgca cgaagtaagg gccagcgtga gggccaatgc cggacaggta cgcctggtga
     9661 atctcacgat gttgaacatg ccggggatta tgaaccttaa cggaggggaa agtcaaactg
     9721 ttcatgcgcc ctccggaaca atgatttttc tcgcataaaa cgcgtatgcg gctacaatgc
     9781 ccgcggcatg atgcaattat cggagatcgg aggccatctg acggccagaa cgggtattga
     9841 tgatttgatg gaggatttgt tcaaggccct ccattcggga gacgccggcc tgtgccagtt
     9901 gaacggcggg agccccgccg ttattcctga ggtgacggaa ctttggcggc gcagcatggg
     9961 ggaactggtg cgaaatggca aatttgacgt gctggtgggc cattatgccc atccgggcgg
    10021 ggatcctgcc tttatccgtg ccctggtgtg ttttttcaat gaacgttgcg gatggaacct
    10081 gacgccggaa aatgtggcga ttacccaggg tggccagatg gcctgcttta cactgttcaa
    10141 catgctggcg ggcccgtgcc cggacggtgc tgtgcgtgaa atcctttttc ccctgtgccc
    10201 ggattatgtg ggataccagt cccaatccct gtgcggcggc gtcatgttcc ggggcatccg
    10261 gcccggcatc cgcatgctgg acgggcacac gttcaagtac gtgattgatt ttgaccgtct
    10321 ggacatacgt ccggaaacgg ccgccgtctg catgtcccgg cctacgaatc ccaccgggaa
    10381 cgtggtgacg gatgaggaat tggggcttct gcgggaaatg acttcccgcg ccggggttcc
    10441 cctgatgatt gacaatgctt acgggcctcc gttgcccaat atttgttttg tgcccgtaac
    10501 cccggcctgg gatgagaaca tgatcctgac catgagcctg tccaagatcg ggttgcccgg
    10561 aacgcgcacc ggcattgtca ttgcgcgccc ggagatcatt cgtgccgtcg tgagcatggt
    10621 gactacgtca tccctgtgcc cgaacaatct gggccaggct ctggtgacgc cttatctgga
    10681 agacggcact ctggagagag tctgccgtga aaccctcacg ccgttttacc gcggccgggc
    10741 ggaattcgcc ctgtccctgc tgccggaact tttcggagaa tccatcccct ggcgcgttca
    10801 taagagtgag ggcgccatgt tcctgtggct gtggtttgag ggattgccca tcacgtgcca
    10861 ggagctttac gaacggtgca ggtcacgggg ctgttttgta aatcccggcc atcacttttt
    10921 cttcgccctt ccggaggagg gggaaccctg gccgcaccgt catgaatgca tccgcatcag
    10981 cttcacccag acggaggaac tcctgcgcaa ggggctttcc atcgtggcgg atgaagtgcg
    11041 caaggcctac tcccgttctt aatccaaacc tgacccgtat ccatgaacag catgaccggt
    11101 ttcggcagag acgccgccca gacagaccgt tataatatcc ttgttgaaat atcaggcgta
    11161 aaccgcaaac aaacggaaat cgccgtcaat gtgccgcgcg gctatgcgga atgggacgcc
    11221 tccgtgcgtt ccatcgtgca gggggctgtt tcccgcggtc gtgtaggcgt ctccatttct
    11281 gtggagcgtc tggaggaagc ggatggctcc ctccagcttg atgaacagaa actggcctcc
    11341 ctggcgaagc tgctgaaccg tgcgtctgac ctgacaggtc aaccgttgcc cctccaggcg
    11401 tccgacctgc tgaggctgga tatcgttact tccgcagcgg aagcatccct ttctccggaa
    11461 gaagcgtggc cgatggtgga aaaggcgctc aaagccgcct tgaaggattt tatttccatg
    11521 cgtgcggcgg aaggcgccaa cctgaaagcg gacgttcttg gcaagctgga tgtcttagag
    11581 caattccgcc tccgtatcgc ggaacatgcc ccttccgtgc ctgtcaggct tcgggaggcc
    11641 atgctcaagc gcttggctga tgccgacttg tccgtttccg cggacgatga acgcatcatc
    11701 cgggaagtgg ccctgtttgc ggacaagtgc gatatttccg aggaaattac ccgtctctcc
    11761 tcccattttg accagttccg caccttgtgc ggttccttcg ctcccgcggg caggcctctg
    11821 gatttccttt gccaggaaat cttccgggaa ttcaacacca tcggctccaa ggcgaacgat
    11881 tccacgctgg cccatctggt tgtggctgcc aagacggagc tggagaaaat cagggaacag
    11941 gttcagaaca tcgaatgaca gccatgaaac agccattggg aacattgctg gtggtatccg
    12001 ggccttccgg ttccgggaag acgacgttgt gccgccgcgc gacggaaaac ggtctgtgtg
    12061 tgtacagcat ttcctgcacg acgcgccagc ccagacccgg ggaagtcaat ggagtagatt
    12121 accatttcct gactcccgcc gaatttgacg ccagggtgga gcggggagac tttctggaat
    12181 atgcggaggt gcacggcaac cgctacggta cgctgaaagc ggatattttg actcttctgg
    12241 aacaggggaa aaacgtggtg atggatattg acgtgcaggg ggctgggcaa gtccgttcct
    12301 gccgggaggg cattctaccc ctctgctatg cggacgttta catttatgtg ccgcaggcgg
    12361 agcttaaaaa ccgcctttgc ggacggcaga cggatggcgc tgaaaccatt gccctgcgtc
    12421 ttcgcaatgc ggaacaggaa gacgcctgcc tgccgcagta ccagtactgc ctggtttcct
    12481 ccgaccgcga gacggattat gcttcctttt gcgccctgct caaatgccag tccatgaggg
    12541 tgggactgat gcggtgataa ggtcttgcct gtttatcgag agactgcgtt ctcacgtttc
    12601 gtcctccgtc gcatggcatc cgggatggcg gctccggttc cgagacattt ggaattggtt
    12661 ctcttcccgt gcctatatga tatgtgattc cctttcctct gatccggcct gcgtgaaaaa
    12721 acgcgggttg aaattcttga cgggcaggat actcataggc tctttgaata attcaatgtt
    12781 gaaattatcc tccaggcaaa ggtgtcccgg taggtcagat actgccccgt ttccttgtat
    12841 ccgttaattg cctttttgac taaaatgttt gactgtcagt tgaacgtgga tggatgaaac
    12901 tactgctttc cgatgctctt ttcttaagga gaaggataat gtaaaagaag agccctgctt
    12961 ttagacgaaa cagggatatt gttttttgtt cccgtgggaa tgatggcgcc tttgaccggg
    13021 tgcttggttt ccttgccgtt gaccgggcct tccaaagcta ttatcgctct acttgagcgg
    13081 atacaaccag ccgatggcgc aggtaaaaat gacccagagg agcaggttga gggggagtcc
    13141 cactttgacg aagtccttaa atttgtaacc gcccgcattg tacacgtaag tgttggtctg
    13201 gtagccgatg ggggtggaga agctggcgct ggcgccgaac atgacagcca gaatgaaggg
    13261 aatggggttg gcgtcgaatt gcagggccat ttcataagcc agaggcccca tgacggccgc
    13321 tacggcattg ttggagatca tctccgtcag gatagagcag atcaggtaca ggccggagat
    13381 ggcgaccagg caccccaacg ggcctatatt atccactacg ccgaaagcaa tggctttggc
    13441 aagcccggtt ttgctcatgg cttctcccac gcacagcatt cccagaatca gaaaaataat
    13501 gccccagtcc accgcctggt aggcttcctt cggcttgatg cagccggaga tgatgacgat
    13561 gagagctccg atgtaggcga gatagaaggg attgaagcgc gcgaagaatt tgaagaattc
    13621 cccgtattgc tcaaaggatc ccagcaggcc gataaaaatg aacagcccca tggccaggat
    13681 ggcccagccc tgtttgctgc ggttgtgcgc ttccgcagga cgctggctca gggggatgat
    13741 gcgctgcttg gtcaggatgc ggttcattcc ttcctgcggt ccttccagaa gaagcgtgtc
    13801 ccctgccgcc agcttggtgt tcggccccat gtccgtgatg tttttgccct gcctgtggat
    13861 ggcgagcacg aagatgttga atctctggcg cagcttgagt tcggacaggg acagcccggc
    13921 gaattcggaa ttgttggcga tcatgccttc cacgatttgc acatcgcgct gctccagcgt
    13981 ttccagcccg cggctgtcat cccagcccag gtccacgcct ttggcttccc tcacctggtt
    14041 gacttttctg gcgttgcaca ggaaaagaat gcggtctcct tcttccaggt tgatgtgctg
    14101 cagctcttcc tgcatggaga atcccctgcg gcgaacttcc acgattttgg tgcccagcaa
    14161 ctcggtttgc atcaggttga tgggagtggt gccgatgtgg ggggaatccg cgggaattct
    14221 gacctggagc aggaaatcgc gctggatgcc gcccggaagc atggtggaca aagtggggcg
    14281 ggatggaagc catttgcggc ctaatgtcca gaggtagagc aggcccgccg ccgcataaat
    14341 caggcccatg ggcgttaccg tgaacatctg gatgccgtca taacccagtt tctgcacctg
    14401 gccgagcaca actacgttgg tggatgttcc gaccacggag caggtgcctc ccagaatggt
    14461 ggcgtaggag agggggatga gcagcttgga ggccgcgatg ttatgatccc tgcagaaggc
    14521 cagcacgatg ggcatcagga tgacgaccac cggggtgttg ttgacgaagg gggaaaccat
    14581 gaacgctccc agcgtgatga ccagcagggc ggtcagttcc ctccccttgg ctaccttgtt
    14641 gaacagtttg gaaaggtctc ctattaatcc cgttctttcc agggaagcgc tcagaatgaa
    14701 catgcacact actgtcagcg gtccgctgtt ggcaaagctg gacagcacat cacgcgtgct
    14761 cagaattccc gtgagcatga ggacggcggt gcctgtcagg gctgtgattt ccacgggcat
    14821 ccattccttg ataaagctga tgaataacag gaccagcaga atgcctacaa tccattgctg
    14881 ggcggccgct gattccagcc agcctatgac ggaggcggag ttgatgttga gaagaggaag
    14941 catggtttga aagttgaagg tgtggggaaa gggtccaact ctcctacttt cccggcttct
    15001 atctgtcaag ccgcaataat tttccggaaa gggtttgaaa aggttcctgc gcttttttat
    15061 tgttggtgcg ccgcatggat tcccccataa taacaaggtt tgctcccagc cctacaggca
    15121 atctccatct ggggcacgcc ctggctgcgt gggaagcccg ttccctggcg aaccgttttt
    15181 ccgggaagtg cgtgctgagg atggaagata tcgaccagac gcggtgccgt ccggagtttg
    15241 ttcagggcat attgaaagat ttggagtggc tgggcatccg ctttgacggt cccatgatgg
    15301 ttcagtcttc acgcttcagc gcttatgaac acgctcttca gacgctgaag gaccggggcg
    15361 tgctgtaccc ctgtttctgt acgcgccggg agattgcgga ggaggtggcc gccatgggtg
    15421 gggcgccgca ggggggctgc gtggacgtat atccgggaac ttgccggctg cttgacaagg
    15481 ggcgcaggaa agagcttctc aaatccggca aacctttttc ctggcggctg gattgccgtg
    15541 ctgcggcgcg gattaccggt cccctgctat ggagggacat gcggttcgga gatcaggtct
    15601 gtcgtccgga agaactgggg gatgtgattc tgggccgcaa ggactgcgcc gccagctacc
    15661 atattgccgt ggttgtggac gacgccgccc agggagttac ccatgtaagc cgtggtgagg
    15721 atttattccc tgtgacgggc atccaccgga cgctccaggc cctgttgggg cttcctgttc
    15781 cgcagtggta tcatcaccgg ctggtgaagg atgcttccgg caagcggctt gccaagaggg
    15841 acaggagcct gagccttcag gaaatgcgtg ccgccgggat gcggccggaa gacgtgttcc
    15901 ggctgatgcg ggagagctga gggattttca ttcccttata ggcgggggtg cgttgttccg
    15961 ccgggaaagg caggggtcag gctgtggctt gcgtttttcc agcagccggt cggatggttt
    16021 tctttccgat gcagagcagg catgcataag ccgccgccag ggtcagcagg agaagggagc
    16081 tcaggatggc cagcagattc agatgcccca gcgggatgag ccatgcgtgc ggcatggctg
    16141 ccgttcctgc cagtatctgg ggaagccaga tgaaaaccgc ctgggaaaac aggctgacgg
    16201 ctgtgaaggc cagccccgcg gccagggtga gccataccga cggaagtact ggcaggaatg
    16261 tttcatggat gttttttttc atggcccgtg ccgttcctgc gaatatggaa atgagaaaca
    16321 ggggggccag caatgcggcg gcagggaggg tgtcccaaag gagccatgtg cagagggggg
    16381 agacggccgt caggaggccc gcgctcttaa tcaggaggcc ccggggcggc ggggctggtg
    16441 ccgcggcgtg gttcatatcc gttaatcggg ccagacattg ccgggcgtgc ttccctgggt
    16501 aatgacgact tcctggccta ttttcagggt gaagtttggc gttacttcct gctgtacttc
    16561 cacaagttcg ttcgtatcct tgagcttgac gcgcacgcgg atcattttga cggcttccac
    16621 cctgccgtcc acgatggagc ctgtggcgga agccagtttt gaggtcccca tgccgatgat
    16681 gccgggagtc atggcctccg ccgccgttcc ggccatgttg gcaaaggtgc cgtcgtccgt
    16741 ggaggtaagg acgttgattt ccttgatgcg gaccacgttg cccagcttga tggtcgtcac
    16801 tttgccggtg gcgatttcca gattgctttt ttgggtgcag gagaaaagga ctgggagaat
    16861 aagaaaagcg ggaatgaatg gaagcggatt catgatgaac gtagttaact acgctggcgg
    16921 ggttaagcgt ttttcagcgt gtgggaagaa ttgtgtaggt agccagatac aggagcaaat
    16981 ggagggagga gagtgccagt accctgttca tgcggctgtc cgcgggcgtt ttgcggatta
    17041 ggagcatcag atacccgccg aagaggatgg agggtatcca catccaccac gtgtgggaca
    17101 ggttcaggtg aagggcccgg ctggatgtgg gcagcgtgat gtaggcagcc aggatgaaag
    17161 actgggccat ggcgcgcgcc tttccttccc ccagccgcac ggccagcgtg cgcttgcccg
    17221 tagtgaggtc ttcctttctg tcgcggatat tgttgacgga tatcatgacg gcgcacagga
    17281 ggccgcattg aatgcctgtt acgatgccgg cattgtacac cgcaagcgat tccaccagcg
    17341 tgccgggtac gacgggcgcc gtgcctatct gcaccagaat ggttcccagt acggcaacca
    17401 gtccgaagaa gaggatgacg aatatttccc ctaggccctt gtaggccagc ggccacggcc
    17461 ctcccgtata gccataggtg aagtagaggg acgggatgcc gatggcgagg atgggccagc
    17521 cgcgcagttc gataagggga aaggccagaa ggcacgctcc cagcaggaag aagaggccga
    17581 tgagcatgac tgttctgtgg gagagggctc cgctggccgt catgcgcacg ggcccggttc
    17641 gccgttccgt atcggcgtgc ttggcatggt caatggcgtc attgaaaaaa ttgcaggcaa
    17701 tctggaggca caggcaggaa gtcagtgtca ggagggccag ggacgcattc cagctgccgg
    17761 tcagtttctg caccaccatg cagcctgccc ataccggaat cagggaggcg gtgagcgtct
    17821 tggggcgtgc cgccagaaag gcggaagtaa tgatattcat gtgagggaag gctccgtaat
    17881 cagcggagga tggggccgag gcggcggagg gtttccttaa tgagtgagac gtgccattgc
    17941 ccgggggccg tttcctcttc tcccaggtag ccatatacca ggcaggaacc cagttgagag
    18001 taaagcaggc gggagacggg tcccatgggc cccattccca tgacggcgat cggccccttg
    18061 ggcttggtga aaagctgtac tcctgtctgg atgtccgagg ctaggcaggg cgtgaatgca
    18121 aatttgacga tatccgcctt gtaggagcgc gctttttttt cctgttcgcg caggtaatcc
    18181 acacccggag tgatttggaa atcatgggtg gagccgatga tgatgacgtc ccgctcccgc
    18241 gcttccataa ccagttccct ggcgtggcgc atggccttga tttcaatgtc catggcggcg
    18301 gcatacggca tataggcccg gagcagggca aagcgctctt cttccgggat gcggcgcagc
    18361 cctccttcct catggcagcg cacggtgacc agcaggggca tctccggccg gaactccata
    18421 acctgttcag tcgtgagttc cgggggaagg gcatccacgc gcagttctac tagatcgcac
    18481 tggtctgtta aatcttttcc tttcagcgtt ttccatagct cccacgaggt aacggacgca
    18541 acgattttcg gcggataggc ctgatgttca cgtacaatct gctgcataca tcctactatc
    18601 ccagcaaagg aaggaaggtc aatcttctat tgatgagttc cacttcattt tggggccgag
    18661 tatgtcagtt cgcctgcctt ttcccctttt ggaactgtgt ttttcttcct gtgccagccc
    18721 tgttggccgg tttcccggca gacggattga cggatgttgg cgccggctgc cgttcaagat
    18781 gccggcaggg ctgtttcgta tggtccgttt ccttgttgtg gcggagggcg ttattttcct
    18841 gcctctgcac gttaaaaaca tgttgagaat ggaggtccgg cttgaaaagg gagttcggag
    18901 ggagctaggt gaaggccatg aatatgcttt ggtcaaggtt attgcggatg gggaggaatc
    18961 ccttttgggc ggctttggat gccgtgctgg tggtaaggac ggaaaaagat tcgtccgggc
    19021 atccggaatg gtcctggaag gcgtatgtcc ctcatggcgt tccggttcat gaattgcctc
    19081 cggtgcccgt tcctcttccg gaaggggatt gggataagga gcgatttcgt aaggaaagac
    19141 ggaaagctgc ccgggtgttg ggtcaccagg ccgctttaac gctggcttct tccctccatt
    19201 tctcccgttt catgattgtg gaggaagagt gccgtttcag gctgccggta ataggttcca
    19261 cggcgcggat gcttgggcag ctgctgaaag gctgccggga aagaactgct ctgtttttaa
    19321 gtgagccgcg tttgtctccc catacggcct gctccgtggc gaaaggagaa aaatccatcc
    19381 tgctgcgcgt gacggatatg gcggcatcag ctgcgtatgt gatggacctt tccttatgtg
    19441 aacgcattct tttatctctt ccgtcagatc agcgggtctg gccatggctg aggtattatn
    19501 nnnnnnnnnc gttatggtga tttatatgct ctttcccctg cgttcgtttc ctgcggagag
    19561 ggcgctccca tgccctccct ctgcaaatcc ctgggccatt tcaataatgc acgcaccaaa
    19621 tatacggatc tgtcttatct ggtgaatccg gcggtcaatt catggcgttg gctgaaaaac
    19681 gtgaacattt tttccggcag aaaaaggctt gggtgagaac cccgggatgc gttttgctcc
    19741 ggcttcgccg gattaccgtt tgcgggggat gaacgcctgc atggctgggg ctgccgcctt
    19801 acggcagagc ttgaaataag gtgttccgtg cgcccgtcat ctcaaaagca aaaccccgga
    19861 ccgcaggcgg tccggggcgg ctcccttttt ccggagggag gatgccgtga tattagaggg
    19921 gttagctggc tccttcgtcg cacaggatct tgacaaagcc ttcctcctgg aggcgggtgg
    19981 cgccgcaggc gaacttggcc cggatttgca gggcttcatc catatcgtcg cgcacggaaa
    20041 gcttcacttt gaaatcattc catatgccaa actgggcctt gctctttacc caggccaggc
    20101 aggaacggac tccctcttct gttttgggaa gcctctgggt gcggatgaat ttgaatccca
    20161 tgaaggtgtc tattttccct tccaccaggg c
//
LOCUS       DAWEMX010000036        20106 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1123_length_20106_cov_9.846890, whole genome shotgun sequence.
ACCESSION   DAWEMX010000036 DAWEMX010000000
VERSION     DAWEMX010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 20106)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20106)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20106
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1123_length_20106_cov_9.846890"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2687..2696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4299 a         5218 c         6086 g         4493 t
ORIGIN      
        1 caagctgggg acggggaccg ttagggcggt tgccgcgggg gccccggtta ccgtcttcac
       61 ggcgtccttc accgcctctg cggccttccg gtcctctggg agcgcggggg ccttcagctc
      121 tggggcctcc cggacgcctc ggaccgtcca tgttgggcat tcccggtcct ttggggccac
      181 cgggaacggc atggtgcggg ccgggctggc cgtgatgagg gggaatggac tgcaattctt
      241 ccggggagag cttcccgtcg ccgtctttgt caaatttttt gatcatggcg gcggcgtggg
      301 cttcctggcg ggctttcatt tcttccatgc gcttggcagc ttccggatgg ttttttgccc
      361 aatcttcccg catggctttc ttttcctcgt cggaaagctt gccatcgcca tccttatcgt
      421 acttcttaag aatttcggct ttcctggctt cctggcgggc tttcatttct tccatgcgtt
      481 tggcagcttc cggatggtcc ttaagccatt cttcctgcat agcctttctt tcctccttgg
      541 aaagcttgcc gtccccatcc ttgtcaaatt tcttcaggat ggcggctttc ttggcttcct
      601 gggccttctt gacgtcggct tgcagaacgg ctatttcctc gtcggaaagc tttccgtcct
      661 tgtcggtatc gtatttggcc aataccagtt cttctccgac aatcatgata ccgggatcga
      721 ttctcttcat ctgaacccgt tttggcccct ggcggtctct gcctccggca tggtggcgat
      781 gacctcccct tctttcaggg gggggctgca tttcaggagc atcggaagga gcctccggag
      841 cgggaggcgg cggagcgtcc tgagcgaacg cggaacatgc ggcgaaagcc gcggcgataa
      901 tcatttgctt ggtcatatct ttgaatggtg aggtttgatg cgtttcggaa aggagcgtgc
      961 gctgcggtgt ttcaaacagg cggaaacgtc tccctttgct aaaaggaacc cctggaattc
     1021 attccggttg caaaataaaa aggcaaatgc gtcggagagg gggtgcgccg ctgtttttat
     1081 gacacctgtt ttcttccggg gtaaagaagg gtgaacccat tgaatatgaa ggaaatcagt
     1141 gaggggatac gcaatcgggc gggagtgcgt gccgcccgtg tcatagcgga aaaaattatt
     1201 cgccggaaat aaagcggatg taggcctgcc accactggct gccgcccagc agccagatca
     1261 gagcggcgct gagaagagcg gggccaaagg ggaggtttct gccgaacccc atgcgtgagg
     1321 cggcagcctg tacaatcccc agcagggagc cgaggaaaag gctgaacagg gcgccctgcc
     1381 agccgcacgc gcatccggcc atcatcagaa tccaggcgtc gcctgatccc atggcttccc
     1441 tgctggcatg gatatccgtc agatttccct ccacgctttc cagtctttcc agaagaaaaa
     1501 cgtccccgtt ttccatctct atcctgtcgt accgcagcgt gacggagcag gcagggtgcg
     1561 ttttgccgtc aatggtgacg gaacctccgg aaaggacggc cttgtccgta gaccggtgga
     1621 aaagcatgga ccagtcgtga ggctggccgt tgacgatgag ctgaagttcc tcccgctcgg
     1681 attccggttc cctcaaactc cacggggcgg gaacgtcaaa atgctgtttc caggtgccga
     1741 acagaagctt ccccaattca atcaccagcc tgatgaccgc ataccctgcc gctcctccca
     1801 gcacggatgc cgtgaaggcg tccgtccagg tcatgacatg gctgtccggc aggaggaaag
     1861 ggtaaagggc gcaggccccc gttccggcca aggcgccgat aaatgcctga gagcggaaga
     1921 caatcagatg ttccgcgtca atgaacatga tgacaatggc catggcagtc cacaggcaga
     1981 gcaggacggc tgctcctgtg gaagcgtccg cgtatttcca gccaatggcc gcgaagagca
     2041 gggccgtgag cagctccaca aagcaatatc ggaagctgat gcgcgttccg cagcaggcgg
     2101 atttaccccg gagagccagc cagctcagaa ccggaatatt caggaaccag ggaatgggtt
     2161 ttccgcattc cgggcagaag gaacgcgccg gatcgttgac ggagacgccc cgcggcatgc
     2221 ggtagataac cacgttcagg aaggagccta tgcaggcccc catcaggccg aaaaccacgg
     2281 gaatcaccca tgcgggaaag gcgtcaggca tgggtgggcg gggaataggt tcggctgggc
     2341 gtctggttga tgcggtcaat cagcaacctc agcctgccgt agttgccgcg tttgatgggg
     2401 aagacgagcc ggggcaggcg gtccaggtgt ggttcgtccg cttcatccgg cagggggccg
     2461 ctctggatca ttttcccccc aggcagaatc aggtctgaaa agtcccgttc cagatgttcc
     2521 acccactggg cgggaagctg cgcattcagc cggatgacga tggaatctcc gacaaaacgg
     2581 taggagtgga aaatccggta aaaacggtcg atgtgttcca tggcttccgc cggattttta
     2641 gtgacatgga tgagatgaag atcgtctgcg gaaatcagtc cggaatnnnn nnnnnnacgc
     2701 gaatgaaggc cagccagttc agccagaagg ttttgccggg ggaatccaga aggacgatcg
     2761 ggtaaatggt cgcttttccc gtctggatca gagtgagtgt ttcaaacact tcatccatgg
     2821 ttccgaagcc tccggggaat gccaccatgg cgtcgctttc cgccacgaag ttcagttttc
     2881 tgacgaaaaa gtaataaaaa ttgatgagtt tgtcgctgtg ggccaccaca tggttggagg
     2941 tctgttcata cggcagggtg atgttcaggc cgaaggagcg ttgctctccc gccccttcat
     3001 tggctgcctg catgatgccc ggtccgcctc cggtaatgac catgtagccg tgttcgctgg
     3061 cttccctggc gaattccctc gccgtttcat acgccggttc gtcattcctg atgcgggcgg
     3121 agccgaagac ggaaatcttg cggatgtggc ggtacggggc gaaaacgcag ttggcgcggt
     3181 acatctcccg cccggagcgg atcaggacgg taaaatcatg gtccgaggcg gcggactggg
     3241 ccatgttgat tcccgttttg atatactctt ggaggtagcg gggatcgtgc ctggtggagg
     3301 ctatttcgga aagcttttta acggcgcctt ccagagtctg gtagctggaa tccatggaca
     3361 tggtggaggg gcgtttcttg tcttcacctg taaaatccct gttgtccaaa aaatcaggat
     3421 gcttcatgag ttggtatgat aggtttgccc tactatgcca gatgacgtac attaagtcac
     3481 agaaaattta ccggcgggca tgaaaagagt tgattgttga aaacagatcg ttacattgct
     3541 ccccgcacgg gcgttcccac aacatgccgt tggtccttca ggcgcattgg ccgccatgtc
     3601 cgtgttgagg ggaaacccgg tacattacat acgattatgt cggaaaacga aaccactact
     3661 gcagaagaaa ccgccgttac cacgctggcc cgttttgagg ttcccagccg tctggaaaaa
     3721 attgaagatc cgaatgatgc gaatcatttg acctttgtgg cggaaccgtt tgaaaacggt
     3781 tacggccata cgcttggcaa ctccctgcgc cgcgttcttc tgggttctct tgaaggcgcc
     3841 gccattacct ccgtccgcat cgccggtgcg cagcatgaat tttcctccct gcccggtgtg
     3901 gtggaagatg tgacggaaat cgtgctgaac ctgaaaaagg tcaaattcaa gcataacggc
     3961 aaggaaccgc gcctgctctc tctgcgcgtg cataagcagg gcgttgtgac tgctgccgat
     4021 attgccgacg acaccgttta ccaggtggtc aatcccgatc aaatcatttg cactctggat
     4081 caggacacca tgttcgaatg cgaattccag gttcgcgtgg gccgcggttt tgccaccggc
     4141 gatgaaaaca aggtgccgga tatgcccatc ggcgtgattc cgatcgactc cattttctct
     4201 ccggttaccc gcgtgaagta ttctgtgcag aatacccgtg tggggcagat gactgactac
     4261 gacaagctga tccttgaagt ctggacggac ggccgcatcg cccccgcaga cgccatgctc
     4321 caggcttccg ccattctgcg ccatcacctg gacgtattcg tcaactatga cgacaagcag
     4381 gtcaactttg aggaagctcc ttccgccgtc caggacgagg aaacggcccg cctgcgcaag
     4441 ttgctcaaca tgagcgtcaa cgaaatcgag ctttccgtgc gtgccgccaa ctgcctcaac
     4501 aatgccaata tcacgaccgt tggccagctt gccatgaaga gcgaaagcga aatgctcaag
     4561 taccgcaatt tcggcaaaaa atccctcaac gaaatcaagg acaaattgct ggaactgggt
     4621 ctttccctgg gcgttcaggt ggatccatcc ctgcttaccg gtcccgtgcc gcaggccaag
     4681 ccgcgcctgg gtctggaaga ggaaagcccc gtgggcctgg cagacctgat tgccgccaac
     4741 atcgaagacg acgacgacta aatctcacaa aaccaacaca catagaaaaa tatcatcatg
     4801 agacacggtg taaaaacctc caagcttcag cgcaatgctt ctcaccgcag ggctctgctc
     4861 gccaaccagg catgcagcct catcctcaat ggacgcatca ccaccaccct ggccaaggcc
     4921 aaggccctgc gcccctatgt ggaaaagctg attacgcttg ccaagcgcgg tgacgtgcat
     4981 tcccgccgcc tggctaccgc taccattcat aatacgaccg ccgttaagcg gctgtttgat
     5041 gaaatcgctc ctctttgcgc ggaacgcaag ggcggctata cccgcatcgt caagctgggc
     5101 cagcgtctga ctgattccgc ccttgtggcc atgatcgaaa ttatcgatct gccccgtgag
     5161 gctgccgaaa aggaagaagc tcccgccacc acggaagcca ccgcctaaga cgcacctctg
     5221 gaacatcctg ttaatacccc gctccggcct tttgtgaggc cggagcggat tttttattgg
     5281 caataaggaa gttatggcga taatatcgct cttgcttctt tcctgccgga acgttataat
     5341 ccagctttaa ctatggacgc agtattattg ttttccggac aaggtgccca aaaagtaggc
     5401 atgggcaaag atttgtatga ccagtatccc gtcgccaaag ccctgattga acaggcggac
     5461 aaggcgcttg gagaatccct ttctgccgtc atgtttgaag gccccggcga agaactgacg
     5521 cgtacctgca actgtcagcc cgccctctac gtacatggcc ttgcctgcct ggccgtcttg
     5581 aaggagctcg ttccctccct gaatcctgtg gctgccgccg gcctttccct gggggaattc
     5641 acggctcatg ccgctgccgg gacctattct tttgaagaag gcctccgcct tgtccagaag
     5701 cgcggcgcgt ttatggagga agcatgcaac gccacgaaag gcactatggc cgccatgatc
     5761 ggcggcacgg atgaaaacgt cattaagctg gcgcaggaat gtgacgtgga tgttgccaat
     5821 ttcaattgcc ccggccagac agtcgtttcc ggtacggagg aaggtgtggc caaggctgta
     5881 gcaggggcga aggccgccgg ctgcaagatt gccaagaagc tcaatgtggc gggcgcctac
     5941 cattcccgtc tgatgtacag cgctatggta aagctggcgg aagaactcaa gaatatttcc
     6001 ttcggtacgc cgtccatgcc tgtttattgc aattatgaag cccgggtagt ggaaggtccg
     6061 gacgacatcc gcagcatgct ggaacaccag gtatgcggat ccgtgcgctg gacggcttcc
     6121 atgcagaagc tggtggacca gggccaccgc cttttcattg aactggggcc gggcaagacg
     6181 ctggccggca tgatggctcg catctgcaag gaagctacgg tcatctccat tgaagacgtg
     6241 cccagcctgg aagccgccgt tgcggagctg aacaaataat ttccctgaat aaaagggggt
     6301 aagccgccgg cccgttgggg agccggcggt tttttgttgg cgtgttcatg gcggggttcc
     6361 gtcaaatagg caatatccca tggaacaggg gaaatgcctg cctccgtgct agcggccctg
     6421 ctcctgcgcg ggcattttcc tcatgccgcc ggtgggaaga tgcatgaaca tcagcatcgt
     6481 tcccatgaaa aaagcgatgg agggcacgaa cagcggtttc catataagga aaagtgcggg
     6541 catgatggaa tgcgccgctc ctcaggtcag aagaatcagg gcaatgaggt aaaacaggag
     6601 gccgctttta ggaggccggc aggagagcat gaaagccgag gctctgagcc gcgccaggcg
     6661 ttcctggcgg cgcagccccc tgcatcaggc atgaaaggaa ttccgtcagg aaacaggcgt
     6721 tatggaatag aaattcctga tttctaaaga agttccgtca gagagcggat gatactggtc
     6781 gccagggaaa aatccgcctg ctccgttacc ctgtttggaa ccgagatgac tggcattcct
     6841 gcgcggtgtg cggctgtagt gccgttctgg gagtcttcca gcaccaggca ttcagacgga
     6901 gatttaccca ggttttccgc ggctttcagg aacagggccg gatccggttt gacggggagg
     6961 ccgtcgtcgc ggcagacgac ggtttgaaaa tagggcatga tgcccaatct gttcagccag
     7021 ccgtccaccc agcggtggga agaactggag gccactccca taggggttcc ggcttccccc
     7081 aggttccgga tgagttctcc ggcgccgggc aggaggccgg catgttccag atcccggacg
     7141 atttcctcct ggcggcggct gttgacggtt tcccagtcga aagtgcgccc cgtcaatttc
     7201 tccagatgtt ctccgggatt ccagtgggtg tagccgctgc ccaggcattg gttgaacaaa
     7261 tccaggggaa gagggtggcc gcaggacgcg aaaacccgtt cccaggagga ataaatggca
     7321 tattccgtat ccaccagcaa tccgtcaaaa tcaaaaatga cggcgtgtgc ccgccggagc
     7381 agttcacggg ctttcagaac ggcttcccgg gaatcggcaa agtgatcagg aatggtcatg
     7441 gacgggagtc tttttcaagc ctctcaataa ttccgagaag cttctgtgag ccagcttggt
     7501 ttgaaaaagg cggatggcga tgatggtgta gataatgttg ggcatccatg ctcccagcca
     7561 ggtggggagg tagcctgcgg aggccagggt ggggaaaaac tcatacagga acagcatggc
     7621 tgccgccagg aacaacgcaa tgccgatgcc ggacatggcg gagcgcctct ggaacgtgat
     7681 ggcggagggg atggcaataa atgtcaggat aatgcaggaa aatatgcggg cgatcctgac
     7741 gtgccattcc gtctgcaggc ttctgaggta cctggcattt gtcgtgccgg acttgatgag
     7801 ttcttggaga gccggggttc cctgtgtatc cacgcggacg ttgggtgaaa tcagttgcca
     7861 gggtgtttcg ggataatgct ctttcagcgt ttggtattcc tgtccggcaa aaacgggaac
     7921 atcatggagc tggcggggtt cctcctggga ataatttctt ttgatggcct gccggaaggt
     7981 ccatgtcctg gaggccggat cccaggtggc ttcatcggca aacagctcgt atttcatctt
     8041 tccgggggcg ctgaactgtt ccacccgcac ctgccggaaa ggattgccgg gggaatcaat
     8101 tccgggagga ttgcccagat accagatgcg ggattccgca tcatttttgt agatggtgct
     8161 ctgggaagca tggttatttt tgttctggct cagggaggag aacatcagtt tcctgtacag
     8221 ggcggagttg ggtgcccagt ggaacccgaa gatgccgaag tagatggagg caaagacggc
     8281 accgatgatg atgggggtgt tgattcggag cagggaccgt ccggactgca gcatgccggt
     8341 gatttccgag gatgaggaaa gtttggtaag cgcccagagt gttcccagga gaagggtata
     8401 aggcagaatc aggttcagga tcatgggcag ctggctcagg taaaagcgga acgtgcccat
     8461 gaggggtgcg tccaggttca tcaggtctcc cacattgtcc gcaaaatctc cgataatgta
     8521 aatcagcgtc aggatggccg tacacatgat gaaggccgtg aaccaggtgc gcgccacata
     8581 cttgtccagg atgctcgaac ggttgtacag gaacattccc agcgcgggga gaaagcagcc
     8641 cagaaggatg agccagggcc gcaggagctg ggaaagggga tattcggacg gcgtaccggg
     8701 aatttcccat tgcatctggg accattcccg gggcatcaga aaccaggctg ccgctcctcc
     8761 cagcgcaagc aggaggagaa atacggacca ttggcggaaa tgtcgggcct ggattaccat
     8821 gaaaaaagaa ggtggggcaa gaatgccaga tcgggagcgg gaaataaagc gttatttggc
     8881 cgccggacag ggataaaagg ctgcctgaac cgcatgtccg tgcttgcttc gtgaacctca
     8941 gtgcggttcc gctccttaaa gggaggaagt ggctgcggga gatgttccgc ctgatagaag
     9001 ttggctgttg tcaacgtaaa ctggatgaga taaaatcttc ctgacaactg tttcccggca
     9061 acagcctttc catgttcaaa cgtcttgttc aattgtttct tctgtttttt ctggctgctg
     9121 cgggtgttct ggcgtggttt gcttggtacg tctccactcc cctgctcatt cccgtgggag
     9181 gggaggaacc cgcctcttct cccgttgcgg gttccctggt gatggaaccc gtggaagccg
     9241 tttcatccga tggcacggct attgaaggtt atctggtgag cccggggcgt ccggaggctt
     9301 tgtctccgcg ccagagccgc gtgcgggata ctctgaaatt gcgcgggaaa atgccgcatt
     9361 tggaggcaaa accggagctg gttgtgatag ccgcctcttg gaatgaggga gtggaaagtt
     9421 ccctccctct ggcggaaggg ctggcaggag ccggttacac gagcctgata tggaatccgc
     9481 gcgggaagaa caatacccgt caattctgca cgtacggatt aagggaatat gcggatgtaa
     9541 cggtgctttt ggacgccgta tcccggaagc agggcggatt gcctcccgtg gctgccgtgg
     9601 ggcagggatt tggtgcggcc gtgctgttga aagccgcgtc tgaagatccc cgcatccgct
     9661 gcatggtcag catggactgc tttccttccc tgaaaaccgt ggctgtccgg gagatggagc
     9721 aggattgggg caagcctctg tgctatgccg cttattggct gctggatgcc ggggtggatt
     9781 ggcgtgcggg gttcagtacg tttgacgtgg ctcccgtgga tgatgccctg aaattggatt
     9841 atccggtcat ggtggtttgt acgaaccaat attttttctc cacgctggaa gattgcctga
     9901 gcatttacga ttccctgaaa aatgagaaaa aacagcttta tgaatccatc cgggaagggg
     9961 aaccgtatgg cacccgggaa cggactttcc tgcattccat tgaaggaaag aagggggaaa
    10021 aatttgaaaa aatttataag gtcaacgtct atgcggggga tgacgagctg caggctggca
    10081 ttgcggaatg gattcatgag aatacgcgta tgcccatgcc cagggtgttg cctcatgagt
    10141 cttacagtgc gcccgttacg ggtaccgctc cttccggttc atgaattgtt cctttccttt
    10201 tgctgatgat cccgtgagtg cgaatcagga caacaaccga gaactccgcg aaaaccgccg
    10261 caggcgcagc cgttttattc tgcccgcgcc tcgccatctg gcagctgtgg cggcaggggc
    10321 tctccttgcc gccgtgctgg tgcattacct ggggtttatc gttctgagct ggtttgatct
    10381 gggcattcat gtgcacaaag cctccgccgt ccaggaggac agaaagcctc tgcctgaaaa
    10441 aatagttctg aaggcggacg accgtcctgc tccggatgta gcggaagccg ttgaaaaacc
    10501 ggatattgag caacttgaag agattccccc ggaattgccg gatttagagg atatgcttcc
    10561 ggagcaggta gtgattgctc cgggggaaac cagcttttcc gcagatccgg taagtccttc
    10621 ccccccggaa tccctttccc cgaaaatgcc gcaggtggac atgaatgccg tcgcgaaggg
    10681 gttgcccgaa ccctctccgc ctgagatgct gaaatcttcc gccagtccgg ttacaatcaa
    10741 gacggctgaa ccggatatgg tgaatccgga tgaatggtat aacaataagc tcaaaggggc
    10801 gggcggtcag gatgattccc atttgccgga tgggagcaaa tctctttccc agctcatggc
    10861 ccagtcctcc ttgggcaagg acagcgggta cagcaggctg ggagcggatt ttctttttga
    10921 atacaacaag gcggtgatga aaaattccgc caggctgagc atgctccagc ttgcggggct
    10981 gatgatgaaa aatccggata cgattttcat tgtggaaggt catacggaca gctttggttc
    11041 cgaggaatat aatgccgtgc tttccctgat gcgtgctaat gccgtccgcc agtggctgaa
    11101 ggataacggc atagctctga cgcgcccgaa tggggaatgc aggttgtaca tacgcgcttg
    11161 cggctcttcc cgtcccgtcg tttccatcag gggagaccgg gatgcccagg ccgccaaccg
    11221 gagggtggaa atccacatgc gcaagccggg agaggaaata cccgccggga gcctgcccct
    11281 tacgtatgcg gtggatatga gcacctctgt cgcccgccag gtgcgtgccg gagtgggcgc
    11341 ccgcgccgga attccctcct ccgcggcaga aaggaaagcg ccccccgcag ggcgttcatc
    11401 ttctgcctcc ccggaacgcc gggaaaatcc tgcgctgcgt caggaagtaa ttcctgaggc
    11461 ggaacccatt ccggaaacga ttccttcggc agagcctgtg gaggaacgca tcccttcggc
    11521 agagcctgtg gaggaacgca tcccttcggc agagccggta gaggatgaca tcccgctggc
    11581 tgaacccgtc gtgtgtgcgg ggagccgcgc cgtagtgaag ggaggtgccg gattgagatg
    11641 gaattgatgc gtgtccttcc ggttcgggcg ctgccgaacc ccgaacggct ccggtatctt
    11701 ttctcctttg attttgacga tactctgttc actcttggag gccctgcgga agaaaggagc
    11761 atttttttca gaaccatgcg catgctccgg tcgcagtatg gtgttctctg gggcgttaat
    11821 acggggcgcg accctgtcta cctgagggaa ggtctggcgg atatgttccg ggatgatgcg
    11881 gaagcgtttg ccccggattt tacggtgacg atggaacgga atgttcatct ggcggatgcg
    11941 gagggcaggc tgatgcccgg cgccgcgtgg aacgatgact gcgctgttgc ccatgacagc
    12001 ttgttcaccc gttacggcgg catgctggaa tcattgatgg gccagctgga aagccggttt
    12061 tccgggctgg gacttcgcag gcaggacaat gacgcttttt ccctggtggt ggatgatgcc
    12121 tgcggcctgg atgaagtttc ctgcgtcatt caggatacgg ttggtccgta tgaggaaatt
    12181 gtcacgcaaa gggcaggtcc ctacctgcgt ttcagccacc gtgattacaa taagggaaca
    12241 tccctttcct ttgttgcctc ccggttcggc attccgtctt cccatgtcgc cattttcggc
    12301 gacgggcaca atgatctgga cgccatgcgg cacttgccgg aggcattccg gtgctgccct
    12361 tccaatgcgg cgcaggaagt gaaagacatg gttgcccgcg gccatggata cattagcccg
    12421 gaaccgcgca cccggggcgt tctggacggc ctggcacatg gagtgttccc ttatttcggg
    12481 atgaaggcgg aggttttgaa ggaggatatt tgaaaacgga gctttcggat gctgacggga
    12541 gatcggcttt caacatgttt ctttcatcaa ggcgctcccg gaaggggaag aatcgtattg
    12601 acaaggaatg cctgcaatgc agaaatggga aagattcttt cccaatcccg attacatgaa
    12661 cattccctct ttttcccgta gcgtttccag aggttccgcc gtgggctggt ttttagtgtt
    12721 gctgctggtt tgcggtgccg gagccggata ttatctttac caggataacc tggcaaaaag
    12781 aaaagcggcc caggaattga ccgccgaacg taaattgaag gagaagaagg ccagggaagc
    12841 tgcggagaaa cagcgaataa aacgggaacg ggaaatcagg gagaagaagg aaaaggaacg
    12901 gctggcggcc cagaaggctt atgaggaagc gcaggaagag aaggcgcggc aggcggcgga
    12961 agcggcccgt aagcttcagg agcaggccga acgcgaagaa cgggaaaaaa ggagaaggga
    13021 ggagctggaa cggcgcgaac gtgaggaaga ggcccgcagg caggaggaag atactcctgt
    13081 ggaggaagag cctgaaccgg aagggcgttt tccccaaccc gtgaaaaacc ggatgccgga
    13141 gctttccgtt tattctattc cttgcaggga tgatatccag acggaaaaag acaagctgct
    13201 ggaaacatgg tcctgggaca aggcggaaaa aatggagggt atggaggaat tccccacggg
    13261 ttcctctccg tggaaaaaag ggaaagacgc cggacgcatg caggcgcttc tggaaaaatg
    13321 ccgggaatgg aaggatgcca aacttgcctc gctgaaggcg tgcccggcgg cgaaggattt
    13381 tcccggcgtt ccggagaatg gggcccagac ggtcaggcgg acggtggaga tagattccaa
    13441 tattggaggc tggcatagta cgggactgta tgcgccgccc ggggcggaaa tttcgtgttc
    13501 cctgtccggc gctcccaaag acggttcgat cagcgtccgc ataggatgcc atacggacag
    13561 ccttcataag ctggatgaat ggaaaagagt gccggaaata accatgcagg ttccggctgg
    13621 ccgtgggcgc gtgaaaatgg tgaatccgat gggcggcctt gtttatgtga atgtaggcca
    13681 gcgtcccaga cggggaaagg tcttcaaggt tcagatttcc ggagccgtgc cttcacctct
    13741 gttcgtaatg gggaagacca ctccggaaca atgggccgaa caattggaaa ataccaaggc
    13801 cccgtggggg gaaatccgca tgccccggct tattgtcacg atgcccgtgg aacagctgaa
    13861 acaatgtccg gatgttcaga agacggcgga atttctgcaa aaaaacatgg ctcttcagga
    13921 ctggattatg ggatgggata ccaagccgga ccgcctgcat catccgatgc gctttgtcgt
    13981 ggacaggcag atatccgccg gggccgggca ttccggttat cccgccatgg ccacgaagga
    14041 ctggacgaat tccattgcca ccggttccat catccattcc ggaagctggg gtttgtggca
    14101 tgaactgggg cataaccacc aatcccctcc ctttacgatg gaaggccaga cggaggtatc
    14161 agtcaacata ttctccatgg tgtgtgaagt gatggggact ggaaaagact ttgaatcctg
    14221 ctggggcggc ggcatggggc cgtacggcat gagcgcggaa atgaaaaaat atttttcagg
    14281 cacccagact tacaatgagg ctcccaacaa ggtgcagctc ttcttctggg tggagctgat
    14341 gtactatctg gggtttgacg ccttccgcca ggtggctctt caattccatg acaagcctta
    14401 tgacaacggc gaactgagtg atgaaaagaa atgggaatgg gtcatgaacg ctttttccaa
    14461 ggtcacggga aagaacatgg ggcctttctt taagatttgg cgtacgccgg tttccgaacg
    14521 cgctacgggc agaatgaaag accttcccgc ctggcttcct tccaaggatt atccggcctg
    14581 ttataccgca gaggaataag aaggttctgg aacagggggg ggaggaggcg ttgccgcact
    14641 cgcttctgga cggttccgcc ggctggggct aggatggcgg agctgtgaaa tggctgaatg
    14701 actggctggg ctttttcaag ccccttcagg tgcggaacct gcaaatattc tggtcagggc
    14761 aggcttcggc cctgattgga atgtggttgc aggtgacggc catgggtatt cttgtgtacg
    14821 atatttccgg aggctccgcc acggcagtgg gggtgttggc tgccttgaac gctcttcctt
    14881 tctttctggg cggcatgctg cttgccgggc tgggggaccg ttttgaccgg aggaaactcc
    14941 tcatagccgt gcagtgcgtc cagtggctgg tggctgtagc cctgtttctg ctgactgtgc
    15001 tggatatgct ccagctgtgg cacttatacg ccgccgggct ggtgatgggc gtcaatcaga
    15061 cggtgggttt tcccacacag caggcttttg tgggagacct gataccgcgc cggcaattac
    15121 aggaggccgt gggcatgtat tctctggtat ttaatacgtg ccgggctata gggccggcgc
    15181 ttgccggcta cattattgcg gaatggggcg ccggaaccgc ctttggcggc aatgtggcgg
    15241 caagccttcc gctggttggc tgcctggttg ccttaaaggg acgcgttgcg gatacctccg
    15301 ctcccagaaa acagcgcggc gcaggaagga aagcatccgg cctcaaggcg gtgcttgcca
    15361 cgcgcagcct gcttttcatc atgatcagcg ctctgattca gaatatctgc gggcaatctc
    15421 tctatcagat tgttccggca ttgatgcacg gaaatcctag gaataccggc ctcattctgg
    15481 gtgcggtcgg agccggagcc atggtgagca tcctctttgt cctgccgttt gcacgcaaaa
    15541 gcgacagggt gggagccaag ctttcctcag gcaccctgtg gatgggatgc gcactgtgcg
    15601 tggcaggcgt catcccggtg gtggaagtgc aggccctttt gccggtttgg ccacttcttc
    15661 cctctttgtt acttcttcat ccgccgtgca gcttctgtcg cccccggaac gcaagtcggc
    15721 cattctgggg ctgttcagca ttgtcaccat cggtgtgcag ccgctggcgg ccatggggtg
    15781 gggagctgtg gtggatgctt ggggtgtcca gatgacgatt gtcgtggcgg gggggctgga
    15841 agccttgttt tccatttgga tgctgggcgt tccattttgg cgtcatttca aattttctcc
    15901 ggatgattgt ccggagacga catagcgcct tcccttccat ttgcggcagg ggaaggctgt
    15961 cggtcctcat gctgcgggga aaaggggtgc atgtagggtt tcccccccgc ggataattcc
    16021 gggatgcagc cggccaggga agaggaagca ggttttggaa acgtcaggag caggaaccgg
    16081 ctgttagccg tccgtttctg cctcgtgttc ggaatagctg atgcccagca tgcagatatc
    16141 gtcggcctga ttggtgcagc cggtaaattt ggctacgcag gtaagggcct gatggacaaa
    16201 atccttggtg ctgtgggggg aggaattatt gaaatagtca atcagccttt ccacgcccat
    16261 ttcttccccg tcctggttgg ctgcttccgt caggccgtcc gtgtacatca ggatgcggga
    16321 ccccgccagg agcgaaaact gggcattatg gtacgtagcg tcatccagca gccccagggc
    16381 gattcctttg ggcagcggca ttttgcgggc gactccgtcc ggtcccagaa tgatgggatg
    16441 cggatgcccg gctgtagaag ccgtcatgac cccggtttcc agatccaggt aaatataaac
    16501 ggcagaggca aacatggtgg tgttcgcccg ttgcaggatt ttggcgagct ggtggttgag
    16561 ggaagtaagg aaagccgccg ggttatccgc caggtgggac aactgttcca tcaggccgcg
    16621 gagcatggag gcgatcaggg cggcgcggat gccgtggccc atcacgtcgc agacaatggc
    16681 gccgacgccg gaactgccta cgggaaaagc atcgaaccag tctcctgcaa ccccctccga
    16741 aggagtgaaa atatggtgaa aatccgcatg gcgggtcagg ccgtgttccg tatgggagcg
    16801 ggagggaatt tcgtagggaa gcagggcgaa ctggatttcc ctggccaaat ctatttcctt
    16861 ttccagagtt ctgttgcgtt cctccaggat gcgggccgtt tccgtggcct tgatttctgt
    16921 cttcaccagt ttggtgacat cactggaaat acctacaatt ccctttagct ccccgttgcg
    16981 tccgtaccac gggaacttgc tgatctggct ccaggaatca tccttgtgga gccatgtctc
    17041 atgatgaagg cgggcgcgga tggggcgtcc ggtaaccatg atgtccattt cctccttgcg
    17101 ggcaatatcg gacatcgtgt ggtcaaaaaa gtaatcgtcc gtgcgtccgg tcaggtctgc
    17161 cggcgtcggt acgcccagct tattggctgt ggcggtattc gccaggacga aacgtgattc
    17221 ccggttcttg aaatagatac tgtcgggaat gtgttcaatc agggtatgaa acaggtccct
    17281 gtcctccgca gcacgcagtt ccgccgtttt ccgccgggta atattgaccc atacgcccac
    17341 cagcataacg ggccggcgct gcttgtccct gcgggcgagg ccgttgacgc ggacccagct
    17401 ccagcttttg gagttcagct tcttcatccg gatttcaatg cgcatgggca catgggagtg
    17461 gccgtccaga taacggcgca cttcctggga aaaaaaagcc tggtcttcct cgtgtatggt
    17521 aagctccggc tgggtgaaga tattgggggc tttctccttg cggctggccc ccatcatggt
    17581 caggcagcgg tccgtgtaat gaatgacgcc cgttttaata ttccagatat aaaatccttc
    17641 ttccgccgcc gccagggcca ttttgatttt gtcccaggta aaaactctct gggcggaatg
    17701 tctgttgcgt gagggcggca ttgcagttgc ccttaatcta acgacaaaac cgctgtcttg
    17761 gcaagaggca aaataaagga atcctttctg aaaggaggcc tggccggggg aacgcgtatg
    17821 aaggacaccg gctggttttt aagatggact cctatttttc tttttccttt tcctgggatt
    17881 gaagaaattg cagttctttc ttcaaagcgc ttcctacgcg gtgctttgcc agatagacct
    17941 gggcaatcgt gacgccgagc tgccggcgga ccttggccgc gtcccactcc cgcaggacat
    18001 aatagtcaaa aatctggaac tgcttgggcg acacttttgc tttgacgcgt tccagagccg
    18061 cctgaagaag gttattctgc cattcccgtt cccatacctg gtcaaaatta ttcttccctt
    18121 catccgggat tttgtcgatg gggtgttcca gggtatccgt ggcatcaggg ttcatcagca
    18181 tggccgtgtc ctttttccgc ttgcggaact ggtcattgat gcgccagcgg gtcatgttcc
    18241 atagccagag tttgaaggaa ccctgttccg gtttgtaaat atttttcctg ctctgcttgg
    18301 cgatggaaag aatggtttcc tgtaccacgt cccaggcctc gtcttccctt aatccggcct
    18361 tgagggcgac ggaataaata agccgccaat atgtccggta aaattcatcc caggtacgct
    18421 ggtcttccca gttttccaaa cgcgcgatca ggcttttcct ggtatgttcc gcgtattggg
    18481 aaagtgtctg ctcgtcaggc tgggactcgt tatggggttg caatgggggc acattcatgg
    18541 tttatcatga actacggatg gaagtgaagc gaaagccgag taataggccg gaaaattctg
    18601 cacggtccag cctgttcatt cactacgtcc gtcgtggaga aaatctttca gggaaacgga
    18661 catgatcagg aaaacgtcaa aacgcagggg aacctccgtc attgcggccc tgattgcgtt
    18721 gtgcgcggtg gtggggtacg gtttgacgga gtgggcgtcg ctggataatg aaccgcaggc
    18781 ggttccctcc cgccagaggg aagagcggca gacggttcgg gagaaggtgg aaatccccga
    18841 caagggggag cccgttcgtt tgctgaccat gaacgccggc aactactttg tgccggaaga
    18901 cccgaggaga agcaattttc aggtaaaata caagcctgtg gaagcccgtg aagccgtggc
    18961 ggagctggtt cgccaatcgg gggcggaaat cgtggggctg tgtgaaatgg gcggggaggc
    19021 tgccgttcgt gacttgcaaa tgcggctgaa aagaaaagga gttcatttgc cgtacaaagt
    19081 tcttgtcatg cgggacgggg aggaccgtgg tttggccctt ctttccaaat accgcatcgc
    19141 ggatgaccgt tccgtaacgg acatgcctgt atccggagag gcgaaacgga aaaagacaat
    19201 gctgcggggc attttggacg ccacggtcag catgccggac ggacggctgt tccgcctggt
    19261 gggcattcac ctgaaatcac gcctcagccg tgacggttcc gcagaggaca cacggagaag
    19321 ggaagcctac gccctgcggg actacctgaa tgaagctctt gcctctcagg acggcatgcc
    19381 tctgcttctg tacggagatt ttaatgacgg cccgtcagac agcgccgtgc aagtcatcca
    19441 ggggccggcc aaaacggaat accgcctgaa ccgtttgaag cccagggatt ctcgtggtga
    19501 gacctggacc atttactacg aagacggtga cacctaccat tccttcgacc atcttttcct
    19561 gaacaatact ctgaaaaagc gcctcggccg caagcctccc atgggcatcc ttgactctcc
    19621 cccctcgctc caggccagcg accaccgcgg cgtgtgggtg gaattaagat aagcagggcc
    19681 tgatgttttt tcagcagggg agatggaaag ggaaaaaatg aaggctttgg aaaaggagga
    19741 caaaaaattc ttttcttgtc gttccggcct tatccttctt gaaatgcggg agttggaccg
    19801 accggatgtt tttcttttcc ggaaggatgg cggaagcagg gatttccgca tggcgattcc
    19861 ttgccggaat tttgttggac aggaacatat caggcattcc agttcctcaa aaaatgattg
    19921 ccgtgtccgt ctgaaacggg tagcatgcca aaaatcgttg ttcaatcctg catcatctgt
    19981 atgagatatt tttccaagca ttccggaatg gctccgcgct gttcctcgcc ggggagcaag
    20041 gggtttaccc tggtggaatt gattgttgtc attaccatta tggttgccat gatggccttg
    20101 gccgcc
//
LOCUS       DAWEMX010000037        19003 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1208_length_19003_cov_13.072039, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000037 DAWEMX010000000
VERSION     DAWEMX010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 19003)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19003)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19003
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_19003_cov_13.072039"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7232..7241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12935..12944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3950 a         4970 c         5880 g         4183 t
ORIGIN      
        1 gtggaaccgg gaatcatttc cgcaaggtcg aacgtaaccg ttttttcgga aatcacccgg
       61 tcaatggcct gaatcagcag atcggcggct tccgtccagc ccatgtagcg caacatcatc
      121 tccgcagaca ggatgacgga gctggggttg gccttgttca gtccggccag tttcggcccg
      181 gtgccgtgcg tagcttcaaa aatggaatgc cccgtcaggt agttgatgtt gcctccgggg
      241 gcgattccga tgccgccgac ctgcgccgcc agggcgtcgg agatgtaatc cccgttcaga
      301 ttcagcgtgg ctaccacgtc gaagttttcc ggatggagca gaatttcctg aaggaaggcg
      361 tctgcaatgc aatccttgat aacgataccg ttgggaagtt tcagccaggg gccgtcccct
      421 atggggaggg cgccgtattc ccgccgtgcg acatcgtatc cccagtcacg gaagccgcct
      481 tccgtgaatt tcatgatgtt gcccttatgc accagcgtga cgcttttcct tccgtttttc
      541 acggcatagt caatggcggc gcgcaccagg cgctccgtgc cttcacggga aacgggcttg
      601 atgccgaagc cggagctttc cgggaaacga acctttttca tgcggtccgg gaaaatgcgg
      661 ctcatggtat caaaaaacag cttggcatcc tccgtgcctt ccttgaattc gattccggcg
      721 tagatgtctt ccgtgttttc gcggaaaacg accatgttca ctttttccgg agctttgacg
      781 ggagtttcaa tgccgttgaa atagcgcacg ggacgcaggc agacaaacag atccagatcc
      841 tgccgcagcg tgacgttcag gctgcggatg cctccgccca ccggagtggt gagggggcct
      901 ttgatgccga tgaggtacgt gcggaaagct tccaccgttt cattcggaag ccaggtgccc
      961 agattatcaa aagatttctg cccggcaaaa acttcatacc atgcgatgga acgcctgccc
     1021 tgataagcct tggctacggc tgcgtcaatg acacgggagg ccgccgccca tatctcagga
     1081 ccggttccat caccgatgat gaaaggaatg acgggcctgt ccggaacgca aagcctgccg
     1141 ttctgcatgg tgacggctgc accgtccgcg ggaggggtga atgttaaatt ggccatgacg
     1201 tgcgttcctt tataggacgc agcccggttt ggcaagataa aagagcggaa tggggaactg
     1261 tgcttctgga gctttgccgg ctcctgaagc aaccggcctt gaaaatgttt tcctcttgta
     1321 cctcagaacc agcggtcctt cccggaaggt tcttccggct gcccggcttc ttcccggagg
     1381 cgtttgagga aattttcttc agcctgatgt ttcaaaccgc tctccatgat atgcttccgg
     1441 gcgttttcgt tcagctggtt gacggcaatg gcggcatctt cggaagtcag cctgttccag
     1501 atgccggcgt catccttcac gattcgcagg gacccctcca ccatctcaca ggaaatcagc
     1561 ttgccatgaa ggccctctgc cgtaatgggg cctttgtttc ctgcgggaat gataatccga
     1621 agaggttcgg gcccttccag attgatgccc ccgtgggcgc ggtaggccgc tttcagaatc
     1681 agcgttttcc tgctccacca ccaggtggtg gagtaacggt attcatattc aaaagtccgt
     1741 tccactgtta ccaggttggc cactttttca tccttctgca caatcagggc cacgccgccg
     1801 tggttggagg aaaaaccctt attgatcagg gtgaggcccg cttccctgcc ggcttcaatc
     1861 agtttttccg ctacagccat cggcttttcc gccggggcgg tgatgcaggt gcgcacgccc
     1921 cagaacaata cggccagcac acctatcaga acggctcccc tggccagcgc gacaacggcg
     1981 ggggaatgga aaaaggtggg ggaggatccg ctcatgagat gttgtgcgtg gcggaaattc
     2041 agggttccgg gtcttcatgg gagccatgtt ccctgatagg gggcatgaac agggccagga
     2101 aggtgaaggc gatgacggtc aggtcatcta tctgtcctac tcccggaatg aaatccggaa
     2161 tcaggtcaat gggggaaaag gcataaagag ccgtcagagc caggaggaca agcttgcgtc
     2221 tggtgaacag gcgcggttct ccttcccggc tgcggaagta attgatggcc ctgaccagaa
     2281 gctgctgcct ggagccgttg ggcggaatgg gaatcttgtt tttcatggag atgcaggggt
     2341 gaatttaccg tcaatggaaa ggaaactctt ttttatatgg accggaagca gacccggagg
     2401 cgggataacg ggccccttcg tcctgccttg ttccggcaac ggcacgggaa gggatttttt
     2461 tgaagccgtt ttctgtaggc gtcttttcat cagggaagct ctttccttat tgtctcatgc
     2521 actcccggcc gcgtccagag caattcccgg cttcttccgg gaatgagctt ccttgcgttt
     2581 gagagagtat aactgcgccg tgcatgtatt tccttttcct ggcattatca tcggcaaggg
     2641 aagagggcaa gtgccggaaa gcgcggaagg aaccgtaata tttgttctgt ctctgccgtt
     2701 ttccgagcct gttttccccc agccgcaaaa acgttccgca gggtagagga gggcttcttc
     2761 cccattggcc tgcgttccct tcccggcaga ggtccgcttg ggcttgcggc attgacacgg
     2821 cgggaggcac ggggtactct cctgcggcat atataatgat cagttttacc aatatcagcg
     2881 gggccgagga aattggggcc aattgctatt tgcttgagat ggacggcacc aggattgtgc
     2941 ttgacagcgg gatgcacccc aagaaggagg ggaaggcggc catgccggat tttgattccc
     3001 tggaacccaa ttccgtggaa gcagtttttt taagccattc ccacctggac catctgggaa
     3061 cgctgcctgt gcttcaggag aaacagccag cggcggaggt gttcatgacg cccgccgcgg
     3121 cagccctgtc tgaagtgatg ttgcataatt ccgtgaatgt gatgagcgcc aagaggctgg
     3181 acctgggcat tgtggagtat ccttttttca cccataatga tctggacaga ttgagcgacg
     3241 cctggcatgc caagtcctgc aatgaagtgt ttcgcgtggg cttccgccag aacgtgctgg
     3301 ccacgtttta cgatgccggg cacatcctgg ggtctgccgg cgtgatgctg gagggtgaga
     3361 gcggccatac cgtattttat acgggggacg tgcagtttga agaccagagc atgattcccg
     3421 gggctgattt tccggaatcc ggcgtagata cgctggttat ggagtgcacg cgcggcggtt
     3481 tccagcgcag cgcccactat tcccggccgg aagagatggt gaggttcggg aaagcgattg
     3541 cggagacgct ggaacgcggc ggcgccgtac tgattccggt attcgccatc ggcaagagcc
     3601 aggagatgct gttcaacatt caccgtttca agcagcaggg ggtgattccc gccaatactc
     3661 ccgtgtactt cggcgggctg agcgcgaaag tttccctgtt gtacgaccgt tttgccgggt
     3721 tgacgcgcag gcatgaccat gaattcaagc tgaaggagga gattcagacc gtgccccttc
     3781 cgcgcaaggg aaaagctccg ctggtgtgtt ccccggggaa tatttatgtg gtgtccagcg
     3841 gcatgatgac ggaaaatacg ctttccaacg tcatggcgga gcaggttctt ccccaggaga
     3901 agaatgctat tttgttcgta ggatatgcgg atcccgattc tccggcggga ctgctgaagg
     3961 caactcctga aggggaactg gtgaaaatga ggcccaacgg gcagccggtg cgccgcaagt
     4021 gcaccgtgga ctgttttgat ttttccgggc atgccacccg ggactccctg gttaattatg
     4081 ccgtgaagct gaaccccagg caggtggttc tggtgcacgg ggacccggac gccgtggagt
     4141 ggatgcagca cacgctatcc gccaaaatgc ctgattccac catcgtcgtt cctgagccgg
     4201 gacgccgcta cactttcgag ccatgagttt taccggagag agaaagggga ggctgatcgt
     4261 ctttgaaggc attgacggca cgggcaagtc cacccacatc ggccacttgc ggaagtattt
     4321 ggaggggaag gggctggaag tggtgcagag ttttgagccc acacgcggac agtggggccg
     4381 gatgctccgg gattccgccg tgaccggacg cctttccata gaggaggaag tagccctgtt
     4441 cctgaaagac aggagggaac atgtgaagac tctgattgct cccgcattgg cgcggggggc
     4501 atgggttctg ctggatcgct actatctttc catgatggct taccagggcg cgcgcggcat
     4561 agatccggag gttatccgcg ccgccaatga agaatttgcc ccggtgccgg atgccgtggt
     4621 ctggctggac attcccgttt ccgtggcgct ggaacgcatt gggaaccgcg gggaacgcga
     4681 cgcttttgag acggaagcgg gcctggcggc ctgccgcagc gtgtttgcat ccgtccatgc
     4741 tccctggatg ctccgcatag acgcggacgc cgggaaggaa gaggtggcgg caagagtgcg
     4801 gaaggctctt tccgtgcgtt tccccgatgc cattggggca taggcatctc ctttcggcca
     4861 taggccgggg atttttcctt agcgtttcat tcctggcatt tggatgctat tccgcaggcg
     4921 cataagccgt tggatccgga atcccggctt ccctgaaggc ttcccggcgt tccatgcagg
     4981 tggaacagac gccgcagtgg acggctccgc ccttgtagca ggaataggtg cgggaaaagt
     5041 ccacgcccaa atcctggccc atggcggcga tgccgccctt ggacatgtgg atgaaagggc
     5101 gcaggatgcc caggccggca taggttccca ggcgaatggc ttccgtcatg gcggccatga
     5161 attcctcccg gcagtccgga tagatgctgt ggtccccgga atgggccgca atgacgagct
     5221 gctgcgctcc gcagctttcc gcaactccgg cggcgattga caggaagatg ccgttgcgga
     5281 aggggacgac ggtctgtttc atcagttctt catcatagga accgtccgga atatcgtccg
     5341 cgccggagag gagggctgat ttcaaatggg aggagatgga ggaaatgtca atcacgcggt
     5401 gttctatgcc caaactggcg gcctgccagg cagcgcattt cagctctcgt tccgcgtggt
     5461 tggaggcata gtcaaagctg agagccaccc ggacgcggtg gttccggtgg gcccagtgca
     5521 gggctacgct ggagtccaat cccccgctga gcagtacggc tgtttccatt ttgtccatgg
     5581 tctggattac agggggcggt tttccggatt gtgttcggct gttgcggtca gctggatgcc
     5641 gccgcggggg gagaagcggc cttccacctt cagccaccgc ggggaaatgg cggccaccag
     5701 atcatccgtg atgcggttga cgatttgttc attaaaagcc gggtagttgc ggtaggaggc
     5761 caggtagtat ttgagagatt tggtttccac gcacttttcc gccggaacgt aggtgatcgt
     5821 caggtgacag gaatccggtt gaccggtaac ggggcagagg gaggaaaatt catgggtgtc
     5881 cagcgtgatg gtgtagggac gcgtgccgcg gttggggaag gattccagcc tggcgtcgtc
     5941 ggggttggta aagaaggagc tttgagagcc taatagagtc agatggtcgt cggacatggt
     6001 tgttgtagta tggtaatgga tgttcaggtc ccctggacgg gggctccgcc ggggaattct
     6061 ttcacgtaca ggtcctgttg agggaatggg atggaaatgc cgtgttcgtt gaacgcatta
     6121 tagatgtgtt cccgcacgga ggaaaggctg gaggactttg tcatgacggg aacccagacc
     6181 catacgccca gattgacggc actgtctccg aaactgtcca gcaccacgct ggttttcttg
     6241 actttggaaa ggaagggcag ggttgccagc gtttccagaa tgattttgcg cgccctttcc
     6301 acgtccgtcc cgtaggaaat gcccacttcc accttcacgc attcgaattt gtggttgcgg
     6361 gtcatgttgc ggaagttctt gttgaagagc tgggagttct ggaaggcgat gatagagccg
     6421 tccagcgttt cgatcatggt ggagcggtag cccagggagg agacgcggcc ccggtagccg
     6481 tcgcattcaa tcatgtcgcc ctggcgcagg cgacccagca ttagggagag gccgctgatg
     6541 atgttttcaa tggtatcttt cagggcgaag ccgatgccca tgctcaagcc tcccatgacc
     6601 atcagcagcc cgttgtagtt ggcgttcatg atgataagcg ccgtaaaaac gaagagcccc
     6661 cagagaaaaa gggtggagag tgtcacgaag gtggggatga tgcctacttc ataatcttct
     6721 ccatagattt catgcagcgt atttttcccc aaaaatatca agtagttgag aacaatggcc
     6781 atcaggatga ccgtgatgac gctgctccag ctgaaagtga gcagatcctt gatttccgtg
     6841 gttgcgaaaa tattattgat gatgaggtcc cccatgtcga aggtggcggc gggccagatg
     6901 atgctgaaca ggatgaggaa aatggtcagg cagggaagca ccagcttgga gatgaagggg
     6961 cggaaccaca ggatagccct cttgttccgt tccttccggc ggttttcata atggtggagc
     7021 agatcccaca gccccaccag cagcaggatg ctggtcatca gcatgatcca ggtcatcagc
     7081 accagaaagg ccatgaagct gaatccagtc cagcacagga cgctgcccag gatggtgaat
     7141 accagagata cggtggagaa aaaacggtcc agccggggca gcttgtgcct ctgccgcacc
     7201 caggtcagca gggagaagag ggaaaccagc gnnnnnnnnn natatccacg atgatgttgg
     7261 gggccatgaa catgcgcagt agcatgaaga atcctcccaa aagcagatag ggcatgtaga
     7321 tgcggatgcc ggagttaatg gtgccgtacg gaaggcgcgt gaccagggag aacaggatgg
     7381 cgctgaccag aagaaggaat tccgcgccca gggaggctgc gctctccagc aggtagttcg
     7441 gcgtgcgcag tgaggcaatg acgaggatca gcgccacgat aagaatggtg gtgacgttgg
     7501 cgaaagcatg gcgtttgttg taatgccccg tctttttcaa tgtatttcgg aatcccagga
     7561 agatgatgat gcgggagagg cagtaggagc acaggatgat tcccagcagg ataagtccgt
     7621 aatgcttgag cgtttccggg tctgacggaa gctggagctt gctgtccgcg gatgtttccc
     7681 atgcccccat gcacgtttca tacgtcttgt gcggtttgag aaagatgtac cgggccgcgt
     7741 gggacggcgt gtagaagacc ctgttaaaca gggcggtgaa cagtttggcg ctttcattgt
     7801 tgagcgcgtc tattttgccg ttcaggctgc tgaaaagttc tttgaggtag ctgatttgct
     7861 ccttttcact ttggagagat tcttccagct cctggcaggc atacagggct tcgtcccgct
     7921 gaaccaggga ggcttgcgac agggtgtccg tatttacttc ctccagggat gccgccagct
     7981 ttttaatgcg ttctatttcc ttgtccagcc gttcctcctg cccctgaaaa tccggttttt
     8041 gggcgtaata cgtcttcacc aggctggaag cggccttaca gtaaaaggcc agcgtaaata
     8101 ttttctggtc gtccagcgcg taaagcacgg cggacatttc atataccctg gtgtcaaaat
     8161 aattataggt agcatccgct tccctcacca gagtcttccg gatagccatc cttttttcct
     8221 cattcttttt cagaatggtg atttcatggc acagagccat gattacggta tccatttccg
     8281 attggtccgg ctgctcctga ggctgttccg cattggcttc ctccgcctgt tccggagcgg
     8341 aggcaggcgg agccgcctgc tgtgcagatg cagaacctgc ggccagaacc cccgccagga
     8401 aaaaggcggc gcagcggcgg aaaaagatcc ggtaatcagg gatgctcatg aatggtccct
     8461 gttctagggg ctacaggcat tacggtcaag gttatatatt ctcctgctga cggagggtga
     8521 gcttcacaga aaaaaacttt tttcaacgtg aaccgtatgg aaaggacagg gcatcttccg
     8581 gcatcatttc ccgaatcccc ggaagatgcc cttccgtaaa aaggaaacgc accggagaat
     8641 ttccggtgcg tttgaaaaga tgaatactcc ggcgggaatc agtccagaga aaaggaacgg
     8701 attctcctta cggtttcctc ccggcccagc agggaaaaga cggaggaaag gtccgggccg
     8761 ccgcttaacc cggtgagggc cacccgggca gggaacatca cggctcccat ttttacgccg
     8821 ttttccttgg caaaggcttt cagcacgccg atgagttcat gtccatccca ttccggttcc
     8881 tgttccagcc tgtccgccag tttgaaaagc agttccaggg cttccggctg aaccttggag
     8941 acggcttccg gatccatgcc cagttcaaaa aagaaacgaa ttttatccgg gacttccgcc
     9001 agggtctgca cctttgtttg cacggtggcg atggcggcgt ccagaatcgg ggaatccggc
     9061 aggccggctt tcaaacagaa ggggcgtgcc cggagggcga attcttccgc aggcagggcg
     9121 atgatgtgct gctggttgac ccagcggcat ttggtgatat cgaatttggc ggcggagtgg
     9181 ttcacggctt ccagagagaa gcgttcgatc agctcctgag gggagaaaat ttccgtatcg
     9241 tccttggggg accagcccag cagggccaga aagttcatga cgccttccgg cagaaagcct
     9301 tcttccgggt aggttcccag ggcggcgccc acgtcgcgct tggacatttt ggagccgtcc
     9361 tggttcaaaa tcagcggcat gtgggcgaaa acagggggag tgacgccgaa tgcctcaaac
     9421 agctggatgt gcttgggcgt gttcatgatg tggtcttccc cgcgaatgac atgggtcatc
     9481 ttcatctcaa tatcgtccac cacattgaca aagtggaaga tgtaggaacc gtccgcgcgg
     9541 cggatagcca tgtccggggt gttggaggcg tcccggtagt cgatggtgat gtttccgcaa
     9601 atcaggtcat ggaaggtgac gggtttggag cggtcaaagc ggaaacgcca ggctccttca
     9661 tcctcgtaca cgcggccggc gtcctggagc tttttgaaat aggcgtcgta aatatcgttg
     9721 cgctggcttt ggaaataggg gccgcaatcg ccgcccttca tgggcccctc atcccagtcc
     9781 agccccagcc attccatgcc ggtgaagatt gcgcgggtag cctcctccgt attgcgggcg
     9841 ttatccgtat cttcaatgcg gaggatgaag gttcccccca tcttgcgggc aaacagccag
     9901 ttgaacaggg cggtgcgggc gcccccgatg tgaaggtagc ccgtggggga aggtgcaaat
     9961 cttgttctga cgagactcat gatgggtaag agagtaaggg cctgtctgcc cgcgtcaagc
    10021 agatacaggg ggtgcatgcc aaaaaacggg gatgcggaag cagtggattt tttaggacgt
    10081 tgtcggcctt gcggcttgcg tggagagggc ttttaggctt catttaacgc cgtgttcttt
    10141 ttaaaacaca tattccgtct gcgggagaag tggcagatta aaagggaaga tgaggaaaag
    10201 ccctttctgg agcatctgga cgacctgcgg accatgttgc tgaggatggc tctgtgcctg
    10261 accgtcagca caattttgtg cgccgggttt gcttccaatc tgatggatat cctgaggcgt
    10321 cccgtcaacc aggtgtggga tatgtttgaa gaatcccatc ttccggcagg gattggtctg
    10381 gatgcctggg gtaaggccaa ggaaatggca acggccatga cgagcctggg agattcccag
    10441 cgggcattgt ttctccgcca ggtttccccg tcccaggcag atttgacgga ggctgccctg
    10501 gttcttcgcg gcgcgcagcc gctgccggag gacaggaagc aggttttcat cagggaaggc
    10561 agcccctcca cggccgtgag ggatctggcg gaagccctgt attccaaagg agccgtgctg
    10621 gcagacggaa cggggcgggg ggccctcaag atgatgagcg cgttccagcc cggggaggcg
    10681 tttatgctga ccatcaagct ttccctgtat gccggagtgg tcatttcctt cccgcttctg
    10741 ttgtacttac tgctgcaatt catcattccg gggctgctgg aacatgaacg caagctgctg
    10801 tacaagtgca tggctgtcgg cttcggtctc ttcctggccg ggacgctgtt ttgttacttc
    10861 atcgtcctcc cgcgggtatt gacgtttttt tacacctatt ccctggaatt tggcatctcc
    10921 aatgaatggc gcatcggcta ttacctgtcc ttcgccacgc agatgatttt aatgttcgga
    10981 ctggcttttg aattgcctgt ggtagtgatg cccttcgtca agctgggggt gctgacctat
    11041 gacatgatga agtccacgag acgctatgcc attgtggcta tcgccgtcct ggccgcggtt
    11101 atcaccccta cgccggatgt agctaccatg atgctgatgg ccgttcccat gtatgcgctg
    11161 tatgaaatat gcatcattct ggcctgggtg catgaacgca aggaggccgc gcgcacccgg
    11221 gaggaaatcg cccggtttga agaagatttc aacaacaata attctcccta caaccagtaa
    11281 atacatccaa cccacgccaa tatcatgaac cttctggaaa ttattatcct gggcattgtg
    11341 gaaggcctga ccgaatatct gccggtcagc tccacgggcc acctgctcct gacggaatcc
    11401 ctgctgaaca tgtaccagag caaggaagct ctggatgccc tggccgtctg catccagggg
    11461 ggagccatcc tggcggtgct gagcctgtat tttccgcggg taaaggccat ggcgcagggc
    11521 gtgcgcggca ataacctcgc cgggctgaaa ctgctggtca acctcattct ggccttcctt
    11581 cccgccgccg tggtgggcct gtgctgcgct tcactgatta aggaatatct gttcaacaca
    11641 tatacggtag cttattcatg gatcgtaggg ggaggcgtta ttctggcgta ttgcgcctgg
    11701 cgcgccaggg agggccgttc caatgaaggg gactccggcg cctctattga aagcctgacg
    11761 ccagccaagg cgctgacggt gggcgcactc cagtgcgtgg ccatgatccc cggcaccagc
    11821 cggagcctga tgaccatgct gggaggcatg tttgtggggt tgagcgtgga agcggccgtg
    11881 gagttcagct ttttgctggg attgattacc ctgggcgcgg ccacggtcca tgatgcgtat
    11941 aaggacggag ctctgatgct ggaatccttc gggtgggaag cccttgccgt ggggacggcg
    12001 gtcagctggc tttccgcatg gctggctgta aaatggatgg tatcctatct gcaaaggcac
    12061 agtttttccg tattcggatg gtaccgcatc gccattggta tcgtgacgct ggttctcctg
    12121 tccatggggc tggtgcattg aaaaacggat tccggcattc aagcaccgga aaacgttttt
    12181 ctccggcaag gcggttctct cccgcagcgg aaagcgggtt ttgaacaatc ccggagaagg
    12241 ttggccgctg cctggacaaa gccatgacac agggccgttt tgcatccccg gaacaaaaca
    12301 gccctgcgat gaatagcgtt tcaggaaaac ttatctcctg tgcattccgt gggaattccc
    12361 gggattggag ggtttcggtg aaggggatgg acgggatggc gcagatgaat gaggggagga
    12421 cggcctgctg ggtgcgctgg cggaatgggg cggggaagaa ggccgggaaa cgggagaggg
    12481 cctgccgggg cggttgatgc cggagtggcc tggattgggg gcggaaggcc ttgaagacgg
    12541 cagggtggaa gggcggctgg gacgtccgaa atccggtctg gagggacggt tgccttccgg
    12601 acggttgatg acggggcgat tcgtgccgcc gggacgggaa ggattattgg cggaagggcg
    12661 agaaggccgt ccgggctggt tgattcccgg atgatgtact ggcggccgct gtaaattgcc
    12721 gggttttcca aagtccggcc gttgattggg ccgtccgggc tgaggggccg gcctgttgtt
    12781 tcccggacgg aaatggtcag gcctgaagtg gggtttcatc ggcggttttg ccatggggcg
    12841 gcagttatgg ggacggacat aatgcccgcg gtaataggga gccggccccc acgtgggaat
    12901 atagtaattc ggacggatca ggttgatgga gtaannnnnn nnnnggcttc cgaacgtgtc
    12961 gtaggcataa atgggtcttc ctccataata tccgtaaatg gggtagccgg agctgtcata
    13021 catgtacgac acgccggaac ctccgctgct gtaggagcct gacacgctga aattgtcata
    13081 ataaggatcg tagcagccgg acaaagcgca gccgatagcg gccgccagca gtgagttttt
    13141 taagataacg tatgctttca tgatgacgaa acagggcgga ttatattttc cctttgcctg
    13201 aaaagcggca aggaaaaagt acatttgttt aaaattattt tgcttggaaa cgcacttcct
    13261 gttgtcttag actgcaaaaa cgttctacgc gatgggaatt cttcttcaaa ataaaaaagt
    13321 cggcctggct ctgctgtgcg ataacgcccc ttatacgctg gctatgactc tggcttccta
    13381 tggcaggtcc gggctgcttg gctattttga cgaagcatgc gccgtttttc cggaaagcgc
    13441 cgtcaaggtg ctggagacgg ccctttccca tgggctttcc gcggtggccg ccccggtgga
    13501 aggcggtttt ataggagccc tccatacggc ggtcaacgct cttgatacgg attacgtgct
    13561 gctggcggag gatgactgca tgatctggga acacctgcgc ggggaaaacc tggaaaagca
    13621 gttgaaacgg gcattggacc tgctgatttc cggacaggcg gatatggtgc gcctgcgcca
    13681 tgcatggcgc ggttgcacgc gctataaggc cgcttataca tattcctact tttatcccgt
    13741 ggaacagctg gccaccatgt gggtgcatgc ggaaggtttg tcggaagcgc cggactggat
    13801 taaaagcatc cggcgcttct tccaccctct ccgttccaag cgttcgatag gccgttgcgt
    13861 ttatgtggag cagaacccgc atcttcgctt tccccagtac attaccaaga ttgatgaagg
    13921 ctatattatt gacagcgagg ttttccagtg gaccaaccag cctacgctca tcgcacgttc
    13981 cagaatcagg cagattctga cggggctgga gcagatgtcc ggctccatcg ggaaattgcc
    14041 gcaggatttt gaacatgccg tcaatagccc gcgatggagg aacgcccata tgaacatagg
    14101 cgtcatcagg ggaattttca cttaaatgcg tatggaaaac ggcggaaaag cggtgcggta
    14161 tgaatgcacc agatgcggcg cgtgctgccg ctgggccggg gatgtgtgcg ttgagacgga
    14221 tgaaatccgc gagatttccc gtttcctgga catggacgaa caggttttta ttgacacatg
    14281 ctgccggttg agagccaacc ggcgcggatt gtccatccgg gacgcggaag acggcgcctg
    14341 catgatgctg acgaaagacg gctgccggat caatcccgtt aaaccccgcc agtgccggga
    14401 ttttcccaac aaatggaatt ttcccggctg gcgggatttg tgccgcgcca gagaagtggg
    14461 agaggaacgg gaagcataat atggccggaa tgccgcaaga ttcttttctg ctttcggcat
    14521 tattccatgg acggggtgaa atccggctgg aatcttgcgt tttttacagg aactgagcca
    14581 gatcccgttc aaaaccggga taggacgtat tgatgcattc cgcatccgtc actacggttt
    14641 ctccctgtgc gctgagtccg gccaccagga agctcatggc gatgcggtgg tccccataac
    14701 tggataattc cgttcccttc aggggagtgc cgccgtgaac aaccatgcca tcgtcaaatt
    14761 cttccacatc cacgcccatg atccggaggt tggccgccgt ggtggcgatg cggtccgttt
    14821 ccttgacgcg taattcgcgg gcgtttcgga cgatgaagtc tccccggccc agggctcccg
    14881 ctacggccag gatgggtatc tcgtcaatga ggttgggtat ttcttccggg aggaggctgg
    14941 tgccgtgcag ggaatccgaa ccgtacacgg taatatcgcc gtatggttcg ccggcatctt
    15001 ccggagaagt gggcacgatg tccattcgcg cgcccatccg ctgcagagcg ctgatgacgg
    15061 cgttccgcgt cttgttcagc cctacctggc gcaacgtcag gcgggaaccc ggacggctgg
    15121 cggcggccac catccagaag gctgcggagg aaatatctgc cgggactgtc aaatcatggg
    15181 cgacggggag agccggcccg caggttccga cggtaagtcc atccacggtg cagggaacgc
    15241 cgaaatggcg gaacaggcgt tctgtatggt cgcgggtgac ggccggctgg cgcacggtgg
    15301 tggttccgtc cgcaaacatg cccgccaata gaatggcgct tttaacctgg gcgctggcca
    15361 tgggaagcgt gtaggaaatg gggtgcaccc gcccgccgtg aatgctcagc ggagcacagc
    15421 ccggtttggc tccccgggct tcaatccgcg ctcccatctg ttccagcggc tgcatgatgc
    15481 ggcccatggg gcgggagctc agggaagcgt ccccaaacat ttccgaatca aaggggcagg
    15541 cggccagcat cccggccagc agcctcatgc ccgtgccgga attgccgcag tcaatgggcc
    15601 gttcaggagc cttggggctc atggcgacgc ccgtaattcg gaagcggacg gggccgtatc
    15661 cctgccgttc ttccagcaca tccactttgg cgcccagctg ctccatggcg cgcagggtat
    15721 tcaggcaatc ttcactgcat agaaaattat ccacttccgt cactccttcc gccaggcctc
    15781 ccagaatggc ggcgcggtgg gaaatgcttt tgtctccggg cacggtcagt accccttgca
    15841 gggaggaaat ggaatgggaa tgaaggttca tgagtttccg gaagaactac cctgtatcgg
    15901 atgcagaatc aagccaatta cgagatgggc ggaggcatca gggagcattc cagaatggag
    15961 ttgattttcc gggacggtgg cgtatattca tgtgcgttat cggctgcatc cccctgaaga
    16021 gaaggagaga gcttttcagg atcaatggat caagctggtt ttcataggta gtaagttgga
    16081 gcggccacgg gagcaatcct gtggccgctt taccttgttc agagggaaga gggcgcccat
    16141 ggcttgaaga aggaagcgga aggaagaaag aaacaaaatt ctttccatgt ctgctttggg
    16201 ctttaccgcg agccgatgaa agcgtatgta gtccatgccg atcaggttcg ggtggtcgct
    16261 gttccggtct ccggaccgga gtagaggaaa gtccggacat cacaaggcag cgtgtcccgt
    16321 gaaagcggga gacgggaatc tcccaagggt tcccggacgg aaagtgccac agaaaacaaa
    16381 ccgcccggat ggggttcgcc cccatgccgg gcaagggtga aaaggggagg taagagctca
    16441 ccgctccgag ggtgaccaag gaggcatggt aaaccccgcg cgatgcaaga caaacagggg
    16501 aagggtcgcc tgcccgccgg atccccgggt attagtcgca tccgttccgt gaaaacgaac
    16561 gggcgcgcgg aacgcctgac tgcggtccgc gtgagaaaaa tgaccgcaca gccggctctg
    16621 gccggcggac aaaatctggc ttacagaacc tggtcggctc ctgctttttt accatgccgt
    16681 tttcctcccc tcttttcggg ggaagagaaa catccgggaa attgtcccgt tccgggggct
    16741 ctttggggtt gcatgggcgt tttaaatgtg tagaatccgt cacctaattg caacccacta
    16801 tggagaagct tgtcgtacat ggaggtttta cgctgagggg agccgtcaat atcagcgggt
    16861 ccaaaaatgc ctccctgcct attttggccg catccctgct gacggatgag cctgtagtgg
    16921 tgcgccgggt tccggatgtt tccgatacca atttcatggt gcagatcatg ggccagctgg
    16981 gagcctccgt ggagcggtcc agcggcaatg tgcgcgtgga agccaggaac ctgcattccg
    17041 aagccgctta tgaacaggtg cgcaagatgc gcgcctccat ctgcctgatg gggcccttga
    17101 tggcccgcat gcagcggtgc gtgattcccc tgccgggcgg ctgtgtgatt ggcgaccgtc
    17161 ctgtggatct gcatatcagg gccatccagg cattgggggc acaggtgcag attgagcgtg
    17221 gcaacctgat tattgaagct cccagggggt tgaaaggagc cacggtggat ttgagcggcg
    17281 accacggccc caccgttctg ggaacggaca acctgatgat ggccgccgta ttggcggaag
    17341 gcaccaccgt tattgaatcc gccgcctccg agccggaagt ggtggatctg gcgaactttt
    17401 taaccaagat gggcgccaat attcaaggtg cgggaacgcg ccggatcgtc attgagggag
    17461 tggaaaagct ccgcggctgc aaccataccg ttatcccgga ccgcattgag gccggcacct
    17521 ttatggtggc ggcggctatg atgggggatg gcgtgaccct gcggcgcgtg tgtgaggaac
    17581 acatgactgt ggtgacggat ttgctccgga agtgcggcca ccacgtggaa ttcaacgagc
    17641 ggggggatac ggtcaccatc attgccggga aaacgcccaa atgcggggaa atcaagacag
    17701 ccccgtatcc cggctacccc acggacatgc aggcccagat gacggctctc ttcgccacca
    17761 cgccgggcat ctccgtggtg aaggatacta ttttcccgca gcgtttcatg cactgctccg
    17821 aactcaagcg catgggggcg gacatcaagg tggacaacgg cactgccgtc atttccggag
    17881 tggaaacgct cagcggcgcc cccgtcatgg cctccgacct gcgggcctcc gccgccctgg
    17941 ttctggcagc cctgaaggcg gaaggcacta cggaaatcca ccgcctgtac catattgacc
    18001 ggggctatga aatgattgac gaaaaactcc tggccatcgg cgctgcagtg gaaaggctgc
    18061 cggatgacga caattaacgc cgttttaggt cctgattttc cttcacaccg cattttttct
    18121 ttactgtcag ggctccatat gctattcacc gaagtcctat gaacaaggct caactgatcg
    18181 aactgattca aagcaagctg ggtgctgata cgaccaagaa gcacgctgaa gaagctctgg
    18241 ccgctgtgct ggaatccatc aaggaaggcg tgaaggaatc cggcaaggtg cagatcattg
    18301 gctttggtac gtttgctacc aagacccgtg aagcccgtac cggccgcaac ccgaagaccg
    18361 gcaacacgat taatattccc gcctccaaga cggttgcttt caaggcctct tccgccttga
    18421 aggactaaga cggcaactcc ttctcaaggg agtacttttc tttcagcacc cccgtccttt
    18481 gaggacaggg gtgtttttta tgtcccggtg cggatgcaca cggcttgacc gggtgaacgc
    18541 ctccgtctag aataccgcca tgtccagcag ttttggtcag gtgttcagaa tttctacctg
    18601 gggtgaatcc catgggactg gggtaggcgt ggtgattgat ggttgcccgt ccctcgtccc
    18661 ggtgacggaa gaagacattc agcgggagct ggaccggcgc aggccggggc agagcgacat
    18721 cgtaaccccc cgcagggagg aagaccgcgc ggaaatcctt tccggagtgc tggacggcaa
    18781 aaccctggga acgcctatcg ccatcagtgt ccggaacaag gaccaccgct cttccgccta
    18841 tgacgagatg gccagaacgt accggccctc ccacgcggac tatacatacg acgctaaata
    18901 cggcattcgc gcctgggcgg gcgggggccg ggcctccgca cgggaaacca tcggccgcgt
    18961 cgcagccgga gcggtggcca gggccgtgct gaagcaggct ttc
//
LOCUS       DAWEMX010000038        18812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1220_length_18812_cov_10.745535, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000038 DAWEMX010000000
VERSION     DAWEMX010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18812
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_18812_cov_10.745535"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3844..3853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6653..6662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9187..9196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17323..17332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4392 a         5511 c         4785 g         4084 t
ORIGIN      
        1 tgagggtggt ccggttcctg atccagccca tctggtcgcg cagggcgtcc ctctgtgccg
       61 tcccaagcgc gttgaccgtg ctccctgcca ctgctgccat ggtgcgtttc gcttccgagg
      121 ggttggaaga cgtcagggct cctacggcgt catccacctt cttgaggatg gaatccgcag
      181 caagatcgcc gggcaggttc cagagaagtt ctgctccggc cctggaatta aacgtatccg
      241 caaggcgggc atatttattg tccttggaag ccgtggccat caaaacaagg ttgccgtttt
      301 ccatcaccag gtacgcatca ttttcaaatt tcttgaaagc ggaggaggag ggatccagca
      361 ggatagccag cctgtcgctt cctcccagat caatggtgcc atccgcgtgc atgagcgtat
      421 aacttccgtc tgcgtccaga gtcagttcat tcccgccagt agaactgaga gttatttccg
      481 cgccctggct gatgctgata tttccggtca cgctgataat ggggccagcg tagtccgtgt
      541 tgaaggtaag gttcgtactg gaaccgtctc ccagcgtcaa gctgctgagg gtcagcgccg
      601 tattggctct cgggttgtct accgtcccac ccatccggat attgagaagg cccgtgctgt
      661 taactgccca gctgctgccg gcagcggtca tgacgttttc aaacgtctgc ccggcattgc
      721 cggaaatatt aagctgcccg cgtccctcca gcgtaccgct gaaaacggaa ccgccgcccg
      781 aattaccggt attgacggtc aaaacgcctc ctgcgctctt cagtgttccc tctccgccga
      841 ttccggaaac ggtgctgccg gatgtcgaac cgaggtcgag cgttcccgtg gaatcgtcat
      901 cttttgccag atcaagaaga acaccgccct gaagggaggc tgttccggaa atttccagcg
      961 tatcacggag ggtcagcgtt ccgctgccct ggatggtgct ggaggaaaga ttgctggcgc
     1021 cgttgacgga aagggaggct ccgctgccga tgttcagggc tgcgccttcg ccgctgtccg
     1081 tcaaatcacc catgacggta ttccggttga tgtccagaac actcccttcc tctgcgccgg
     1141 caagttccac atgattaaag ctgttccctt caccgttaag agcgagggaa ccgccttcca
     1201 cacgcagggt gtccgtcaca aaagaaccgt tgactgtcag cttgcccgtg ttttgcttga
     1261 cgaaggtgac tccgttttcc ccgacaatgc ttccgcccat gatggaatcc gcaccggcgt
     1321 aatcatcagg agcaggcaat cccgtttcca gacgttcgtt gttgaggatg acaacagcgt
     1381 tgcctccgtc cgcactggtg ttttccactt tcagcgtgct tcccgtagcg cccagcaggt
     1441 tgttgatgac cgcgccgtcc ccgtcggaat cgccgggtgc gccggccaac tgaacggtaa
     1501 gcgtcttgtc ctgggaaatc accacgccgg agtacatccc cagcgtgccg tagttggtga
     1561 cggtatcagg atcggaagta gaggaatcat ccaccatata aagcccgctg acctgcccgc
     1621 tgagcaccag gcttcctcca tcggtgcccg tcacgcggat gccgtaattg gaaagaatct
     1681 tgctgaactg gatatcgcca aggttttcgc attccagcgt tccggtcgtc agggtgagcc
     1741 agctttccgc accggcttcc ttatgggcga cgagcgtgtt cacaatagcg tcaatgtcca
     1801 gattgactgt ggcgggattg ttgatgacaa gcctgccgtc tccctggtca atgatggccg
     1861 ccccggccgc atcctggttg atgttatccg ctcccaaggt aagattcaat gtttccgtgg
     1921 ctcccgcccc caccgtaata tctccggaaa cgttggcaag cctgcctccg gcagccagcg
     1981 taatgccctt gagcatgctg ccgtcagtcc cccccaaatt gacagaagct gctgagtcca
     2041 cgacaatggc tgccgtggcg ccttccgccc gcgtcatggc ggaggtcagc gcgccattga
     2101 cgattttcag cgttcccgct ccattcagca cggttccggc ccactggccg gccttgtctt
     2161 ccgtgccaag ctggactacg gctccggcat ttgtaatgcc gagcgctccc gtagcggtat
     2221 tggcctgatc cagaatcaga gtgcccgtgc cagcgtgcgt caggctggag ttgccctgca
     2281 ccagagcgtt caacaccagt tccgtattgt tgtagttgac gttgtattgt gcggccagcc
     2341 cggtatccgt ggaccccgtg ttacctccca gaatagcgcc gtctccaaaa acggaattcc
     2401 cttcacccgt ggcgcaaacc ccgccggaac cgaagttcca ggcctggcga ttgttgttgg
     2461 cggaagtctc attggtggac agggtccccg agaagtcctt gagggatccc gtgaggacat
     2521 tgttcatccg aacgttcgca gccatggccc acgtacccga accggaaagg tttccggaga
     2581 aagtgtatgt ggcgccggag gagccgttct gcatcgcacc gtgggttgcg ccgatttcaa
     2641 aattggccgc cgtcgtaccc gcggaaaaat ggaagccctt gccgtcgatg aaaacggtac
     2701 cgcctcccag cgtatttcct gccccaaagg ctacccagtt ggcattggta ttcatattca
     2761 ccccgttgcc gaccagttcc aggcggccag taaagttggt attgcccgta ttggagatat
     2821 tgaccagtcc cgcagtatcc gcagtcccga tagtgacggt gcccgcccca ttgagctggg
     2881 catgcaggtt gacggttccg ccggaaagag caagggaagc gccttcactg acgcggacaa
     2941 tagtagcatt gttcggattt acgccgatag tcgcaccgcc cgtgaaagcg agcgtgccct
     3001 cttccacagt aaggtgggcc aaggtattcc ctgcgtatcc aagggccagc gtgcccgctc
     3061 ccgttttggt cagggtagtg ccggccccca tgacatcggc tgacaagccg gcccaggaaa
     3121 cggtatcccc ggcgccaagc acggaaacgt tcaggaaacc tccgtctccg acgttgacac
     3181 ggctggaaat gtcataccct gtcgcatcct caccggcaac ggaatccgca taggccagcg
     3241 tccccccgtt gaatataata tccccttccg ttcccagcgc tccgtcagcg ccaatggtaa
     3301 gggtaccgcc attgagtatc gtccctccgg tataggtatt gaccaggttt aaggcaactt
     3361 cagccgtacc gttcacggta agcgtggtgg gagctgtgga atccgcaata gatccctcgc
     3421 tgtcaggatc gctgaccaac gtgtaagcgc cgctgttgaa aacaacgctc ttgggagtta
     3481 caatgccgga aaccttgact tcacccgctt ccgcggagga gaagtaaatt tcctgattat
     3541 ccagcgtatc cgcccccgtc agcctcgtcg tccagccgtc aacactggtg ttgatccact
     3601 tccggtccgt tcctccgttc cagacaaaac cattgtcccc gctgacgtca gcaaccttca
     3661 gcacaagctg gttattttcc accaccacgg aatattcgaa cccttccctg gtgatgtcgg
     3721 gcgtccatgt gaaggaatcc gtcgtcaggc ttccatccgc agcccactgg gccaggacat
     3781 agttagatgc ctccaattca ccataattat tgatagtcag cgcacaggac gaatccgtaa
     3841 gagnnnnnnn nnngccaggg acccggccgt gatattgatg acaggctcat tcgccaccac
     3901 gcccgtcatg tcaaaagtga gggtagcgcc gctattcagc gtcagagcgg aagccgtcgt
     3961 cattctggcg cccgcaccca tggaaatgct gcccagggcc atggcattcg tattcgtaaa
     4021 cgccagcgtt ccggcctgga cattagtcag gcccgtatag gtattctgcc cggaaagggt
     4081 caaggtgcct aagccggttt tcgtcaggcc gcccgctccg gaaaggacgc ctccgagggt
     4141 gatgccggtg gaagcctggt catggtagga cttggtttcc gcatcatact ggcgggtatc
     4201 aaccgtcaca tttccagtaa gggacatatt gtaggcggaa gaccagcctt ccgccgccgt
     4261 cgtccccagc gtcccggagt tcatgttcag ggcaatcgta ttggtatttc cggatgccgt
     4321 catgccgcct gaaccgagaa gcagggaacc gccattcaga ttaatggtgg cgcccttggc
     4381 gcttactcca tcccagttct gccccaaatc cagcgtctgg gccagcacca tgccgcccga
     4441 accaatattc atgacgcctt ttccgtcacg ggcaatccgc agcgtgccgg taatgttcat
     4501 cacggcgtcc cggctgatgt tgagcgtggt tgttccattg tactcgtagc ggccgatgaa
     4561 gcttcccgcc gtcacggaag cgccggcccc cagcgtccag gtacatgccg tgccgttggt
     4621 tgccagcacc ctgtactctc ctgtaatatc aacatttcca ttaaggacat tcgttcctac
     4681 cgcggatatc agcgtaccgg ccccggtaat cgtatttcca taggttccgg cggaaagggt
     4741 cagcgtggta ttggctccca gctcatacct gactccagtc agggcggccg ttgtcgttcc
     4801 ggaggcgttc ccgctcaatt tcagcgtgcc tgaggaattg acgttcaaac gtgttcccaa
     4861 cccgttccac gtccccgtgg tcagttccag aacggaatct ccctgcaaca ggaccgtgct
     4921 ggtgcccagc gctccggcat tgccgacaac gagtttgctg ccatcgccaa gaacggtatc
     4981 cccggcatag gagttggcgt tgttcatggt cagggaagct ccgtccagca ggttcagcgt
     5041 cgtatccccg gtaatggaac cgtcacccgt aaaaatgaaa tccgtaccgg aaacattgat
     5101 tctgcctgga gcgacattcc ccgcaatcgt cacattttta tccacgccat cgccattgga
     5161 gaaatatacg gtctccccgt cggcataagc tgacggtgtc ccgtcaaggc tccaggaagt
     5221 attcgagctt tccgtattcc aagtgccgct cttccacctc aataaacctt ccttgtcact
     5281 caaattgacg taaagagtgc cgttgctgac ggaaaacgtt ccattgtaca actccgcagc
     5341 agctccgccc aaagtaaaga aagaactttc cgtcaaccct gctccggaaa gcaaggcata
     5401 actaatcccc gtttccaggg aagacagccc cagccgtact tccgtagcct cactgccgct
     5461 gatggagttg acggtcaggg ccgccgtctc cgaatcaaga gaactgaagc tcagggtgcc
     5521 gccgtttaac gtcaacccgg cggataaagt agccgttcct ccggaaattc ccgtggagcc
     5581 cagcgtggaa ccggcattca gcacataccc gtccgcggac gtaacgttca cagtaagacc
     5641 gcctccgatc agcaaagccc cctgcccgat aacgctggaa tccttcaccg tcaaaccgcc
     5701 ttctctcaat tccactgttc cggtaaagcc ggggacggct gctgtttctc cttcggcagg
     5761 aaccggggcc tgggctgttc cgttaagaac cagaaccccg tcgccaacct tcacgagctt
     5821 accgtctccc ttcagcccgt tggagaaagt cacagtacgc cccgtggcat ggtcattggc
     5881 atccagcgtg tcaaggatag tcccattcac cgtgcccaga agttctatat aagatgccgt
     5941 gaaatccgga ttatagctta taccccagtt ggacaaagcc cccagagtgc cttctcccag
     6001 tttgataacg gccgctcccg ccacattggt aatgcccgaa gaacctatgt tcaccctggc
     6061 atccccggac gttgcgccgc ccaacaggaa actgccccgg aatgaaccgc ttccggaagc
     6121 ccaaaaatcc attccctgca aattggccgt tccggacgcg gcattgaaaa tgccagtgcc
     6181 atcccaggac agatgcatcg aggactgcaa tgcagtcaaa gaaccgcccc tcaaattaag
     6241 ttcccctgaa taagcccagt gagccagcag gaaactggcg cttgtgctat gggtattatt
     6301 gctgccggtg acagtcagag aggaaccctg gttcacatta agcaccccat gccccagctc
     6361 ggcaagccgg acctggcctg ccgtagtcac attgccggaa tcaagatcaa gagcaccata
     6421 taaggaattg tcatacccac cgccggcccc gacccacagt ttattaactt ccaattcatt
     6481 atcggccata tgaaccctgc cgtaataatg ggctaccttg aacgattccg cctggatatt
     6541 tcccgtaaac gtcaccgtgg aaccggtctg ggcgccagat acgatattcc cggcgtatgt
     6601 gatattccct gctacgggag gattgctgtt cacgttaaag gtccgcgaat ctnnnnnnnn
     6661 nnacgctctc gccgcccacc aggttccaga gatacgtgtt gctgtcggaa acattctgcg
     6721 catagacatt caagctgccc tccgcgatta ccgtttgccc cgctccgatg gatgtccccg
     6781 catcggaatc gaataccatg gtagaacccg ccatcatatg caggttggct ccgccaagca
     6841 tccatgatcc cgactgggaa gaagccccca gtctcaaggc tccataaacg taaagattct
     6901 gaagggccga accctccagc aaattgacgc ttcccgcaga agaagcctcc gttttcatgt
     6961 aaaccgtgag ggaagaattt tcccctcccg tcaccaggct ttcaaaaccg gaaaaacggt
     7021 gatccccgtc acccaacgtc agggacgagc tgttgcttaa aaccgtttcc tgggcaaaaa
     7081 gagtggcgcc ttccccggga aggagcgtat aatccgtctc aaatgtcatg gaggagacat
     7141 tgatccctcc ggagaaattg actgttttgg agccttcctc tgccgtgaaa atgacggcat
     7201 ccccattcag gaaggaagta tccgtcttgg ttccgtcacc ctgcttgtac cagcccgttc
     7261 ccgtattgct ccatgtcccg ttggaattcc acaccagagt ttctgcatca tacccttccc
     7321 ccttgtactg cacggacatg ccggaaccgt tggaaacaag caggaaatta cccgccccca
     7381 gctggccggc ggaatcaacc ctcgtcaatg cctcataagc atcatccccg gtttgcttcc
     7441 aaatacgcag ggaatccagg gatgccccca aatcagcccc tgtagacatg aaatagcgtg
     7501 tgatcagggc gtcgtcaacc atctcgcgat tggtgaccgc aagcccttcc atggcgctgg
     7561 caaccactac ctccacattc catgttttgg cgttcttggt aattgtggtg gtgtttgaca
     7621 ggttaatcat tccgtcaagc ccaatgtgcc agtaatgatt atcggcaccg gtaccattgt
     7681 caaaattgag attccctccc ggagtagtca tgttcagagt gccgtccaca tagatattgg
     7741 catgcccctc tggctccata tttttgagct gggtaccgaa agaagtggta agggaggaac
     7801 ccttttccac gtaaacattg ccaccccatt gtccaagccc cacagacaca cttccgttcc
     7861 ccgtaacgga aataccgcct ccgtccgagg tattgggagg cgttcccgta accgtcacat
     7921 ccacgccatt aaaaatcaaa tgcggaccct tattgtccac gcgggacggc gccgagcctc
     7981 cctcccagtt gcctgccgtc cccacagcgt cgccttcccc agcccccgtc catgtaaaat
     8041 caggaccgcc aaaatcgggg atctgccaga aatccgccga ggttgcggca tgggaataag
     8101 agcaaatgcc ggagaaggaa accagggaag cgagaagggc ggaacgcaag gacggaggga
     8161 gatgaagtct catattaatc aataatattt cagccagtaa tacccggatt tataatcctg
     8221 cttctttcct taatctatgt attttacttg taatgaaaga gtgatggttt tgcttctctg
     8281 gatatgtagg cggatatgct ggacaggtgg agtttattat tttccattat ttatttaatg
     8341 cgagtcattt ctgaaaatag acttgccaac ggattataaa ttcccagtct gcccattcgc
     8401 tttcctgatg aaagcggtac ggataaaaac aaaaaggccg ctccctcccg ggaacggccc
     8461 tgacttgaat tattttttcc ttactgttgc tgggcgccgc tctgcttaag catctcctga
     8521 agttgttccg gagaaatgct catggggcct ccctggggag caggggcttc cgaaatgccg
     8581 tccagttcca gatcaaagat gagcggggaa ttgggttcaa tcggagcgcc gggagtgttt
     8641 tccccgtaag ccagctcgga aggaatgtag acaatccatt tggagccgac gggcatcgtg
     8701 gtcagcgctt cggcaaaacc ggggatgacc tggagaggaa gttccacgga cttgcccttg
     8761 gtgtcgtcaa acacggtgcc gtccagcagc gtgcccttgt acttcacctt gaacatgggg
     8821 ttcttgaact tcttttcatc aaatttttct tcgccgccct tgttcacaac cttgtactgg
     8881 aggccggact tcgttgtggt gacgccttct tttttgccgt tttcctccat gaatttcctg
     8941 ctggcctcca aattggcctt ggccttggcg gaaatgcgtt cgtcaatttg cttctggaac
     9001 gcatccaggg cgggtttcag ctgttcctgg tccttggcgg gcttgcggac catgccgtcc
     9061 ttgatgccct gcaggaagga ttcctggtcc aggtcgtcaa aggtcagggt aggaatggaa
     9121 ccaagttgct gaccgctctg gaaacccaga aaatagccca ggtccttttt catctgttcg
     9181 ggagacnnnn nnnnnnggat tccgttcata tcacaagaca agagaatcgg tcatcctgtc
     9241 atcctctccc ggcaggaggc cggattgctt tccggtcaga aggagaatca ggtcatgcca
     9301 gcgtttccag aacgcgggcg atcatggcgg gataaagggt atgctcctgc tcctgaatgc
     9361 gggcgtgaag gctttccggc gtatcccccg gcagcacggg cacgcgagcc tggcccagga
     9421 tggggccggt atccattccg tcatccacat aatggacggt gcagccgctc tccgcagctc
     9481 cggcgttcac agcctgctcc caggcatgaa gccccgggaa gtctggaaga agggaagggt
     9541 ggatattcag gatgcgggaa ggaaattcct tcagcagcgg aagtttcacc aggcgcatga
     9601 atcccgccag gcacacgcag tccacgccgt attgcttcag gcgcgcggca acggatgcct
     9661 gggattcctc cggaaatctg gttttaaatc ccccgcagtc aataacctcc gccggaatgc
     9721 cccaggaacg ggcgcgttcc agaatatagg cgtccggatt gtcagacatc accacggcga
     9781 tttccgcccg gagagaaccg gactggatgg catcataaat ggactggcaa ttggaaccgg
     9841 aaccggaacc gagtattcct aacttgggca atctggacat ccgggggata aaaccacagt
     9901 tttttgcccg tggcaagtct ccggcttgac aaaaaggctg ccgtatttgc ctaataaagg
     9961 catgagagac ccccgttttc aggcccttgc agcccagctt gcagggtatt ccacagccct
    10021 gcgccccgga gacagggttc tgctggaact gacggatatt ccggaggaaa tgggaatagc
    10081 cctcatccgg gaggcgagga atgcgggagc aatgcccttt ctgcgcctga accagtcccg
    10141 cctgaaccgg gaaatgctct caggagcaac ggaagaacaa tacggcatca tcggccgcca
    10201 ccgcctggcg gaaatggagg acatggacgc ctacattgaa atacgcggcg gggggaacgc
    10261 ctttgaactg tcctccgttc cacaggaaaa tatggccgcc gccatgaaag ccctgaaccc
    10321 cgtctcccag cggcgcatcc gccatacgcg ctggtgcggg cttcgctggc cgtccggggg
    10381 aatggcccag caggcggcca tgagcacgga agagtttgag gatttctatt tccgcacctg
    10441 cctgatggat tacgccgccc tgcgtcccgc catgcggaag ctggccgcca tgatggaaaa
    10501 ggctgaatat gtgaaaatta ccgggccggg aacggacatt tccttttcca tcaaggggct
    10561 gcctgccatc ccgtgcgcag gggaatgcaa tctgcccgac ggagaagtgt tcaccgcccc
    10621 cgtcatcgac agcgccaacg gccacatttc cttcaatacg cccagcctgt tccagggaat
    10681 cccctttgac aacatccgcc tgacgctgaa aaacggactg gtcatgcatg cggaagcggg
    10741 agataaaaca ggggagctca acgccattct tgacacagac cccggcgcgc ggaggctggg
    10801 ggaattcgcc ttcggcgtca atccggccat cacacgcccc atgcgcaaca tcctgtttga
    10861 cgaaaaaatt tccggctcct tccacttgac gccggggcag gcctaccacg tggcggacaa
    10921 cggcaaccag tcccgcatcc actgggacat ggtatgcatc cagacggaag cggccggggg
    10981 cggagatatt tacctggacg ggaagctggt gcgccgcaac ggattattca ccctgccgga
    11041 actggccatt ctcaacccct ccctatcatg accacccacc ctttctccaa aaagcgttcc
    11101 cagctccgca agctcctgta tgcggccgcg gctgtcattc tgctggggtc cctgctggcc
    11161 tcctacatgc actgggaagc caatcatctg gaatttaatg aaaacacggc ccctgccgga
    11221 tgtgtacccg gcctttccgg ccttcgtatc ctggtattgt cggatatcca caccaatctg
    11281 cccctgctgg aaaaagcggc aacaatagcg gaacaggcca gaccggacat gatcgtattt
    11341 ctgggagatc tgtacacgga ttttctgcgg gtcactcatg caggcgacta catcacccag
    11401 atgaaacgcc tctcctccgt cgccccggcc tacgcatgtc tgggcaacca tgacatggcc
    11461 cttgcggaca atgtggaacg cgtcctgaag gaagggggct tcacactgct caggaattcc
    11521 gccgccttcg tctccatccc caggctggga aatgtggaat tcaaactggt ggggctggga
    11581 gacctgcggg agggtgactt tttcccggac cggtgcatga gcccgcgcga attggaggaa
    11641 aactcgccca tgccaaccat cgtcctgagc cacaacccca aaggaaggga attgctggga
    11701 aattaccgct gggacctgat gctctccggc catacgcatg gaggccaaat caaactgcct
    11761 ttcttttcca ccccgctatt ggcgtcggaa ggggaaacca tgtactccgg gttccatcct
    11821 tatgaggaca agcaggtatt cgtcacccgc ggcataggct atatagggcc gggccgcttc
    11881 aactgcccgc cggaaatcaa cctcataacc attccctaac ttcccgacct gaccgccatg
    11941 gttctctctc tgctggcctc cggaggccct aatgacgcgc ctccattctt cattctgctt
    12001 actgtcgtct tcatgggaat catcgcgatt gccctcattc tggcccactt ccggcagtcc
    12061 ctgctggcgg gctacttcat ctgcggggta attttggcca actgcggcat tctggaccac
    12121 atccccaatt ccgacgtcag catccaggct ctttccgagg taggaattat cctgctcatg
    12181 ttcacgctgg ggcttgaatt ttccgtcgat gaactcaagc acctgcggaa aaccgctctg
    12241 gtcggcggag gtattcagat gttcatctgc actgcggctt tcggcctggg gcttcattac
    12301 gccacaggca tggacatgag ccactccctg acggtcgggg ccattatggg actatcctcc
    12361 acggcggtgg cgctgaaatc cttccaggaa ttcggcctga gcggaacctc cggggcaaaa
    12421 atggcggtgg gaattgccct gttccaggac atcgccgcca tcatgctggt ggcaatcatg
    12481 ccgcagatct tcaccgccgc gcccgactca tgggaaacag cgctcaacat cggcatggcc
    12541 gtgctgcggg ggcttgtctt tctgggagcc gcctggctga tcggccgcta cctgcttccg
    12601 cgcatcatgc tggccgtcgc ccggaccaaa agccgggaac tcttcaccct gatggtgttc
    12661 gccatctgtt cgggaattgc catgctcgcc agtctgctgg gcctgagcat cgccctgggc
    12721 gctttcaccg ccggcctctt cgtcagcagc tcttattaca gccaccgggt tcttagcgaa
    12781 gtgctcccct tcaaggacct tttcctgacc atcttcttcg tttccgccgg gctgttgatt
    12841 gacattgatg gactgtggga acacatcggc caggtggcaa ccttcgcctc ctttgtcctc
    12901 gcgatcaaat tcgtagcctg ccttacagcc gcaagcttcc tgaaaattcc cgggcgcatg
    12961 ggcatcatgg cctccaccgc actcaccaat gtgggggaat tttccctggt gctcatccct
    13021 ttcatgcagg aaatatcccc catcccggcg ctggtgacca ccaacgttta cgccattgcc
    13081 gccgtctcca tgggcatgac tccgctactg atgaaggcgg cacggaaact taccccgctg
    13141 ctggtgcgca ttcccgggtt gaaaacaaaa cgggaacgcc tcaacgtgga aaccctgatc
    13201 acacgcatgg aaggcatcag gaaccatgcc atcatttgcg gctacggccc cgtaggcagg
    13261 aggcttcatg aaagcctgtc ccagtacggc atcccctgca tcattatcga cctgaatgcg
    13321 gacaccgtca aatccctgct caacggcggc catctggcct ttctgggaga catccagcac
    13381 caaatcacca tggacctggc gggcgtccgt accgcgcggc tgattgcctt cacattcccg
    13441 gaccctgccc cggccctgtc cacctacatg cagatcaagg gagccaatgc ggacatcacg
    13501 gtgattgccc gcgccaaatt ccgttcggaa gtggcttccc tgcatcacgc gggcatcacc
    13561 aacgtcatcc atgatgaaat ggaaacgggc gccgccgccg tgcgcctggc gaaacagagt
    13621 tttgatatca ttgaaccgtc ggacgcgcca tctctgtccc attaagcaac gatgaatctg
    13681 ccggacgccc acacccattc ctctacagcc gcttccggca cggcgccgca gttcatctgc
    13741 ggcacctccc cggcggactg gaaacaggtg gcccttctgg cggaacggga tgaacacgtt
    13801 acgccctttt tcggcattca tccctggttc ctgaacccgg ctgaatggaa agaggaaatg
    13861 cccaggctgg aatcccttct gcaaaaaatc ccccatgcgg gcgttgggga aacggggctg
    13921 gacaaatgtc ggcgcggcat cccggggctg cctctccaga aggaagcgct ggaacggcat
    13981 ctggaactgg ccgcgcgtct ggaccgcccc gcttccctgc actgctgccg cgcatggggc
    14041 acgctggcgg aaatgctgac aaaatatccc cgtttgaaca ttgtcctgca cggctggacg
    14101 ggggcactgc atcccggcac ggaacttcct tcgggaaact ggcttctttc cgtagggctg
    14161 cgggaaatgg aacgtccggg actccttgcc tccataccgc tccatcggct ggcgttggaa
    14221 tcggacgggc atccggaaac ccttccggaa ctttaccgac gcgcggcgca ggaactgggc
    14281 atgaccgtca acaacctggt tgacctggtc caaagcaata tggaaaacct gatccgccct
    14341 tgacgcctcc attcaacggg cggacatggt ttcgtggcgc ctcttcattc actgggtttc
    14401 tatttccatt gattcttcgt tcaatcattt tcccagaaaa taaaaatatt tccgttgaca
    14461 gttcatcatc tttttgccat atgatggatt atattaggta tggatacaat tccgcccatc
    14521 tcccatgaat aatatcttcc gaattctcct tttaggagcg ctccccctgg cagcctttcc
    14581 cgttatccag gcgtcagcgc aggaaccgga tatccagaca ctactttcag ccgaacgttc
    14641 ctcgttcatc tccggaaaag cccgggatat tctgtgcagg aaactgggaa ccgcaaacct
    14701 tgacacggat aaaatccggg ccatggcata cgccccgcaa gtcgccctgt tatgccacct
    14761 gtaccaattt ttcagcaccg cggaaaacgg agaaccgttc acgcagcatg aactcaagga
    14821 cgagtcattc aggaaatggc tctccaccca cccggaagtc ttcagaatgc ttgccctatc
    14881 cggggcagcc ggcaaacaaa ccctatccat attttaccgc atctggaacg cgaacaataa
    14941 aacgctccgc cccgttgaaa ccagcatggc tctgggggca gggcttgcct caaatgtcat
    15001 ccctcctgaa gaatgcctgt ccaaattcaa tttttaccgg gaatcctact tccaatccgc
    15061 atgtcatccc caggcggaca cgatgcagcc gtgggaatgg gccatcgttt tccgtggcag
    15121 agagagcctg gaagacctgt cctgggccca gcagttcata gagaaaaaac agatccctcc
    15181 cgaacaggct ggaaataaat tcatgggttt catcccctat cgcaggaaaa accttcaggg
    15241 agtcagcgtt catgccggag ccgcttttta cgaccacaaa ccggtcacat taaagcttta
    15301 tacggaatac ggaggcgtgt gcggcgccgt ctccaaagga gcggcaggct ttctgagagc
    15361 caaaggcgtc cccgcgtggg ccatcgggca gccgggacac tgcgccttta tctggaaaca
    15421 ccccggcgga cactggaaaa tcggcaacaa catcagcgga tggaactggt caacgggaaa
    15481 aagccagatc ccctggaacg gccccgtcca gctcatcccg gcttacaatg ccttcattca
    15541 tcacagtctg gcggaagaat ccttcctgat gacggtttta tctgactgct ctccgcgttc
    15601 cgtccaacgg gaattgctgc tgaaagaagc ctgcaagatg aacccattta attatccggc
    15661 atggcgtcgt tacctgagca tgaaggccaa aggaatcaat gaccggcaaa aactcaccct
    15721 gcttaaagaa ctggcgcagg ccatgcctca tgaacacaac ctcctccatc atgcagcggt
    15781 aaacatactc aaaatccggg aaagcaaagt caatccctat gaactgtacg cctgtttcct
    15841 ggactccgac tgctctcccg cggcagagga actcttcacg aggctgtgct ggaacaaact
    15901 cgtggcggat tgccctgaaa tcggcaagat aatcaaatac agggagggat tcatcgggaa
    15961 gcatctttcc gtctgggcca ggaaagggaa caatgcctca tggacgccta aaatgaaaag
    16021 atactccgcc ggcatgatgg aaggcgccat cacggcattg gaaaaaagag aacagacccg
    16081 cacatactat gtggcaacct accgccgtct gctggaaata tggaatgaca aaaaattaaa
    16141 gcatcaaggg gaaatattga tcaacaaaat cagcggcaat aaagtctcgc ccctgcggca
    16201 gaaccggtaa ccagcacaga aaattttaac atgaacgcga acgccatcat ccgggcatcc
    16261 gccccattct tccaaaaata cgtcttatcc ccgggaatgc cggcaaaacg ccatctcgcc
    16321 atgatgctgc tgggcctctg cttcacagcg cctttactgg caaatgaggt attgcgcatc
    16381 gaatcacagg cggcagaata ctctgcccaa atttatattc aagaagaaga aaaaggttcc
    16441 gtaccggaaa aagagaaaga gaacgatcaa aaaagaaaga aattgggcat aggtgaaaca
    16501 gtaatattaa ccctggtttc agacaagccc cagctcattg gagacccatc ccagatcgaa
    16561 tggacaatcg aagaaggaga aaatctggct tcttttcaag aggaagacaa ctctgtcatt
    16621 caaacattgg ttattagaaa tgacctctcc caaggaggcg acattactgt taaagcagtc
    16681 acggaagaag gaagagaagc gaaaatcacg ttatcagcca ttgttcccga agggatcagc
    16741 gcaaaacatt tacggaaatc atatgacaga aatcatccgg attttaataa aagaggagtc
    16801 atttattttg atctgccaaa ggatggtgat caagaaatgg caggggcatc agctgtcttg
    16861 gaattaacgt tcctccctat aagcgtgaat tttcgaaatg tccatattat tgaacgggat
    16921 aagggctgcg ttcccgcccc ggctgaaggc agcctggcag ccccacatga tcccaatcct
    16981 gcaccagtac gccttgacac caaaaacagg ctatctgaca acattggatg ccgagaacct
    17041 attgcaaaat taaaattcta cgacttgcct caaactcata aatggatttg cgactggaac
    17101 atcaatgtcg acgggaaaga tgtaaaaacc attcagactg ttgaacagca ttttttttac
    17161 gactggattg acgtcgtcaa cgaaatagct acgacgacgg tttccaaatt caactgttcc
    17221 gtcagcaggt ccacagaaaa aaataacaag cacgtattta tcaaccagtc aaaataaatg
    17281 aacctaaaaa aatgatgaaa tattttattt acgcgattgc cgnnnnnnnn nntcttggct
    17341 gttcaacttc cctgggggaa gagaccggat ttgatcgcca actcccggct cctgattcca
    17401 tccatattcg caaaaaaatg ggagaaaaat caaaaaaatt aaaagaattt ttaaaatcag
    17461 gagattttaa acaattctat ggatgttcaa acacagttct gtcagaattg aacgccatgc
    17521 ggcgccatgg aacgatgacg cgggaagagg cctgcgacat gctctggagc ctttatctga
    17581 tcagcggggc ccccatgtat gaagctccgg attacgacag cgttcagcca tggccttatg
    17641 aaaacgagcg ggacaacgac attgccgcca aatcaagagt catttccgcg ctctccatca
    17701 tggatacgaa acagatgtcg cgcaacctgg gcatccacga acaacggctg aaacatctcc
    17761 atgccgctta cgccgccgcc atcattaaaa ggctgaaatc tctacacctc cctgatttcg
    17821 gaaaaaaaga gacagcgctg aaagatgcaa taatacgttc ccatcctgcc aatccggacg
    17881 ggaatgtcat cggctctgac gttaaagatt acgaatggac tcacaaatgg aataacctgg
    17941 ttacattttc gtcaagaaac agcatgtatc atagctatgt ctctaaaatc atggagaaaa
    18001 gattcatccc catgctggtg aagtacttcc ccgaccaggc cggggaagtc gtcaaataca
    18061 tcaggaaggc gggatacgga gacggcgagg ttctcgacct catcgaccga acggcaggat
    18121 acaactccaa aaccgcctac ctctaccagg ggaaaagcgg agaggaacac aggaaaaagc
    18181 ttcaacggaa acagagcgga gagtgacgcc gaacgttcat tcattgcgcc atctgcctcc
    18241 tcccaaccag gaaaaacaac ccggacaaga cggtgaacca cgttttccat ataatcatct
    18301 tcatctgcct cggatggagc ctttcctccg cggagggaca ttcattcgat catgaactca
    18361 tgccgcccga ttccatccat atccggagaa gtacagaagg gcatgctccg ctcctgaaaa
    18421 gcctgctgga acaaaatgat ctgaaagcat tttatcagca cgctaaaacg gttctgaccg
    18481 caatcaacaa actgcagcat gacggcgtga tgacacggga agaagcctgc gacatgattt
    18541 ggagccttta tctgataggc ggggccccca tgtatgaagc tccggattac accagtgatc
    18601 aaccatggcc ttataaaaac gagaaaaata atgatattta cagcaaagca agaatcagca
    18661 cagcgatttc ccttatggat ataaatcaac tatcaacaac attaaagatc agtaaaagaa
    18721 aattgataca ttttaatata tcccttcttt caagcattat taaaaaaata aaaaaatgca
    18781 tttcaagtat ttccacaaga attaggaatc ac
//
LOCUS       DAWEMX010000039        18681 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1229_length_18681_cov_10.707720, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000039 DAWEMX010000000
VERSION     DAWEMX010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18681
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_18681_cov_10.707720"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1118..1127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10452..10461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3716 a         4891 c         5899 g         4155 t
ORIGIN      
        1 tggtcgatca tggaatccgc cactttcacg aagccggcga tgttggcgcc ggtgacgtag
       61 ttggtgaagg atttgtcgga ggagtattca cgggcgtggg ccacggcgtt tgcgtggatg
      121 ttggccatga tggtcttcag cttgtggtcc acttcttcac gcgtccagga gaggcgctgg
      181 ctgttctggg acatttccag tccggaggtg gccacaccgc cggcattggc ggccttggcc
      241 gggccgtaca ggattttggc ggccaggtag gtttcaatgc cttccagatc cgtaggcatg
      301 ttggcgcctt ccgccaccag ggtgcagccg ttcttgatga gggtgcgggc gtcttccccg
      361 ttgatttcat tctgcgtggc ggaggggaag gcgcagtcgc acggaacgct ccaggggcgc
      421 tggccttcaa agtactgggc tttcgggaac tgcttcacgt attccgcaat gcgtccgcgg
      481 cgcttgttct tgagttccat gacccaggcc agcttttccg gggaaatgcc gtccggatcg
      541 taaatatagc cgttggagtc ggacatcgtg accgctttcg cgcccagttc gttaagtttt
      601 tccacggtgt actgggccac attgccggaa ccggacacca ggcagacttt gccctgcaag
      661 tcgtcgttgc gggtggcgag catgttctgg gcgaagtata cgcaaccgta gccggtggct
      721 tccgggcgga tgagggagcc gccccagttc aggctcttgc cggtgagaac gccggtgaat
      781 tcgttgcgga ttctcttgta ttgtccgaac aggtagccga tttcacggcc gcccacgccg
      841 atgtcccccg cgggaacgtc cgtatccgcg ccgatgtggc gctgaagctc cgtcatgaag
      901 ctctggcaga agcgcatcac ttcattatcg ctcttgccct tggggtcgaa gtcggaaccg
      961 cccttgccgc cgcccatggg gagggtggtg agggagtttt tgaaaacctg ttcaaaaccc
     1021 aggaacttca ggatgccgag gttgacgctg ggatggaagc ggaggccgcc cttgtatggt
     1081 ccgatggcgc tgttgaattc cacacggtat ccgcggtnnn nnnnnnnttc cagaatcgcg
     1141 tggtcttcat acttggaatt cgcggccaaa accggttcga tcgtgctgag cacttcctga
     1201 acggcttgga tgaattcttt ttcgtggcta tgcttggcct taaccaagtc gagcacgcgc
     1261 tgggaatatg tctgtgccat cttttctgtt gttgtatttg ggttgacgct ttacgcgcag
     1321 tttttcgcac gcgccttccg tttatcattc cgcgaagcgg actggcaatc atttttttcc
     1381 ctggcggact tcaggggtca gtaagaaaat agaaaatatg tttatatgtt gtgatttgta
     1441 aactgaccct attcttgctg attgatgctt gccagaactg cgtattccgg atgctgggac
     1501 atggagtttg tatgcggtta ttcgggttcc tgtttccgta aggcggattt tgaatccggt
     1561 gtccatgaag aatgggtgac aaaggaaaaa attatcagga acagccaagg cggcagagat
     1621 ggaatgttac aaacaacaag ctgacctggt caggtgggtg ggagccatct tgatttccgc
     1681 attggtggca gaaggccgga attgcctcca tggctttttc agtagttccg gaaccggctg
     1741 cggcatccgt gggattgctt gggctggcct gcatgatgat gcgctggaga aggggctgac
     1801 cttgagtgct tcctgccgcg ggaggacggt ttcatgcccg gcgcatcctt gtgtcgggag
     1861 gaaagttttt ccaaattatt ccgtgggagg aacggaggtc aggagctcag gcgctcgatc
     1921 cacgccctcg ttttcaaatc cgtttccgcc attttttttg cccggtggag caaggttccc
     1981 atggatttgg gaatgcccat gtgcagccgg ttgctgatcc aggcatacgg taccagcgtg
     2041 ctttgccgga cggccagggc gatgatgagc ttggacgggt ggctgtaagg cgtgacaagc
     2101 agctcctcct ccgagaggcc gaaggccttc agaccgtttt ttacaataat ttccgcctgg
     2161 tactccccaa tttccgacgc tcgcatgccc gtcgtgacgg gctttctgcg cgccagacgg
     2221 ggaagttccc ccaccagcct ggcacggaag gcgctggagc catagcacca gccccgctgg
     2281 acggtgccgg ggccaacgtg gccggcgggc agcagggagc gttcatccat gcgttccgct
     2341 tcaaaacggc ccatcagatg gttgagatat ttttggcggc cgtcttccgt gtcgtcgcag
     2401 ccgaagcaga cgaggccgcg ttccggatga atccatttgg gccttttggc gggagaggcc
     2461 agccacgcgg gcaggctcgt ccatttgcag ccggaaagga acgtgtggcg tcgcgccagg
     2521 ccggaccggg ccggattcag gtggatgtag tcaatgatgg ttgaaaaata gtgggcgtta
     2581 tccgcttcca ccaccatgga gtggtatctg cccgcaaaca gatgaccccg cattcggtgg
     2641 cgtgcgttaa aacgctgggt ataggcagtc tggagccatt tcatgccatc caccaggttg
     2701 gcgcgcgcgg tttggaaaag aatgtggtag tgattggcca tcagggcgta ggcgtacaca
     2761 gtccagccgc ttctgcgggc tgcctcctga aacgttcgca aaaaggtttc cctgtcttcg
     2821 tcatccaggt aaatggcatc tccgcggttg ccctcgctct gcacgtgata gactgctcca
     2881 gggtattcta ttctcagggt gcggctcatg ccggaagcgt accggaaaag gtctgaaatg
     2941 caagtctgtt gttattttta acctgaccct tattgtgaaa aaagagggtg aaaaaaatgc
     3001 atttctaggc ttgtccgcgt ttcttggaag gagcaaattt ttccggagga cttccgggca
     3061 gaggattgcg gaagaagccg gattctcgtt ccgggacggc ggcagggcaa tttgccgggg
     3121 aatgcatgcc tttgccccgt gttttctcat gaacggagcc ccggatgcgt cgttccccgg
     3181 aggtcatgcc cggattaggg cgtgaaagga aggctggacg ctgcagacac gaagaatggg
     3241 aaattgccct ttggacggga aggggaggcg gccttgaacg ggcatttccc gcaaaccgcg
     3301 gaagggagcg gcgttttttc cggtatttca acggatacag cgcggcaagg cagattttat
     3361 tgcagttttt ccctgcgcat gatttcagag agcaccaggt agaggtccgt cttgttgact
     3421 acgcccagca gcagatcgtt gttcgggttc acaagggcca gggattccgc gcagtggggg
     3481 cgggagaaaa tacccagggc ttccgggagg tgcatgtccg caggaagcgt aggcatgtcg
     3541 ctgcgcagca catccatggc cagtacggct tgggccagct ccttgttgcg ggcaaaggcc
     3601 gccacgtctt ccgccaccac gcttcccagg tatttgccgg ccggggaagt gacgaagatg
     3661 ggctgggccg ggtttttaat gagcatggag gcgatggtgc cgaatttgtc caggggatgc
     3721 accacgggcg gatccttgcg cgctacatcc tgaagctgta cctgggccag gggtttgtca
     3781 aaggtgcttc tggggccgaa ggccagggaa tcatggtaca tggactgggc tttggtgagc
     3841 ctggccattc cgtaggaaac cagcacgccg atgatcaggg ggaacatcat ttgcccggcc
     3901 atggtgaatt ccaccaccag caccagggag gtgatcgggg cgttcgcggc tgtagtgaaa
     3961 aaggcggcca tgcccaccag ggaataggca atggcatggt ccccgggcac ccccagctgg
     4021 gtaagagccg cgctgaacag gaagccgcag acgctcccga tagtcaggct gggggtcagg
     4081 gcgcccccca cggttcccac gccgaagacg atggcgacgg tgaatatttt caggaacagc
     4141 aggacggcgg ctctcgcggt gtcaaattca taatgaatga gggaggtgat gatatttttg
     4201 ccgttcccta cgatttccgg gtaaaagatg gcgatggctc ccaccagcac gcctgccgcc
     4261 gtcaggcgca cgggcagcca ttggcgcttg ccgttcaggt accggcgcga ttttttgagg
     4321 atgaggagcc agaaactggc gaccagggag gccagggctc ccagcagcac gcaccagagc
     4381 acatgctggt tgcccgccag agaaacttcc tgctggagct ggtagatggg ggcgggttca
     4441 tggaacaggc ccatggtaaa gtaccccgcg cagctggcaa ccagcagggg agccaggaaa
     4501 tcaatagtca gcgcgcccag aactatttca cttacgaaca gaccgcccgc caggggcgta
     4561 tggaaggcgg aggacatgcc ggaggcggcg gcgcaggcca ctaccaggcg caggcgagga
     4621 gctgagaggt ggaagaaacg ccccagggcg gagcccgcta cggcggcggc ctgcaccagc
     4681 gggccttcac ggccgatgga tgcgccggaa cctacggaga aaacggcgga cagggaacgc
     4741 agaaggctgg gcttgggagg cacgtatcca ttgcccagcg ccacggcttc catgtattcc
     4801 gtcgccttgg ccggcacaaa gcggtgggtg aagagaagcg taagccccgc caaaaggccg
     4861 cccgccgccg gaacgaagat gcaccaggcc gggtcctgac tggccatttc ctgaaagacc
     4921 tgaatttgcc tgtagctggt ccgccccgtc aggacgtcct gcaccagttc cacgcccatt
     4981 tcaaagacaa gggccatcag ggcacccagc gctccgacga cgaccgccca gatgaagatg
     5041 acctgcttgt cccccagctt gattttttca cgcaggctca acgccatgcg cagccatgac
     5101 caggaaaggc gcgtcttttc cgcaggctgg tttttcttgt ccgtcattgc agggcttgtt
     5161 gaagggacgc caggagactg gcttcatctc ccgtccattc cccgttgggg cccagggggg
     5221 cgtttttatg ccaccgcagc aatttctgca tggtggtcag gtagccggtc accatggcgg
     5281 cgggtacgcc cagcatcaca atgagatgag cgttgcggga atcgtccggc cagaggacgc
     5341 cctctgggct gatgcctacg gtgacctgca tggaatccag cgcgctggtg cgtccgtggg
     5401 ggacggccag gccgtgatcc agataggtgg tctgcgtttc ctctcgcaga agaaccgcat
     5461 tcttgatgtg tcttagctgt cggtcgtcaa ggacttccgc catggaatcc gtcagggttt
     5521 ccagagcctg tgcctgagtg gttgagggct ggaacgtgcg tacgtgaatt tgaagattgt
     5581 ctgccaccgg gtcttctgaa ggcttttggt ttttcattct atgaagggag gcatgattat
     5641 caaggaaatt ctggcctgca tacgggggaa ggaggctttc ccccgtgaat gggaggcggg
     5701 aaaaaggggg aagagacctt tccggagggg cttttttgac acaatggcgg cggaagagcc
     5761 agcttttagg ctttttcttc agcgggaatg aagataccct gccgccatga tgcaggattc
     5821 agaagtcatc aaactcgtca aacccgtacc ttccgaccgc ctggcaaccc aggtgctggt
     5881 ggcatggtac catgcggtgc gccccaggtg gaaaaagccg ctggacctgg ccattacggc
     5941 cttttttgta ttgatggcgc tgtattcctt ttccctgcgc ccactgccct tatttgcctg
     6001 gctgtccctg gccggggcgc tgatggcgat tatcctgtcc tggttttacg tcccctttct
     6061 ggcaaaggcc gctctggccg ccaataaaaa ggtgcccatg taccatcagg aaaaggatta
     6121 cgtttttcat gaggattacc tgacgttctc cagcaagggc gttgacccca tgaatattcc
     6181 ctacgaccgt ttttccacgt tgtgcaggac gcgccacgcg ctgctgttcc tgttcggcaa
     6241 gaagctggtg ctgtggctgc cgctccatct catggatgcg gaacagctgg aaagcatcat
     6301 gcgcaggttc cggaatcatg gcgtggaagt gaaggatgtc tcctgatgcg gaggggcagc
     6361 ccgcgccttc ctgaaggaag gggcaaattt gccgtttcag cgcctttttt gttgagcgcg
     6421 ggatgaaaac gggtagggtg tttccatgtc acgttaccat ggagaacagg ttctggtggt
     6481 tccccgcgcc gcctttgagg aagcgggtta tttccaggga caccgggaag gcggggatca
     6541 ttatctgaat gcgttcatga agccgggcgt cgcctcgttc atggaccggg aggctgcgga
     6601 aaatgaccct tcccacaaac agatcatcgc ctatgccatt ttctgccacc agggccgcat
     6661 tctgcattac acgcgcggag gctccggcgg cgaggcccgc ctgcatgaca agggctccat
     6721 cggcatcgga ggccatatca atccggtgga ccgccaatcc ggccatgacg acgtttccac
     6781 ctatctggcc ggagtggagc gcgaaatccg cgaggaactg gttattgacg ggaactgcac
     6841 ccagcgggtt ctcggcgtga ttaatgacga taccaatgac gtgggggccg tgcatctggg
     6901 cattgtccac ctgtttgagc tggatacgga ccgtgtgcgc gcgaatgaag cggctctggc
     6961 caatctccgt tttgtttctc cggaggaatt gtccggggaa atgtttgaga aactggagac
     7021 ctggtctcag ctcgcactgg aactgtttaa aaacagaggg gcctgatccc ccctgttcca
     7081 tgaaaaacgc ccggggaggg atggatgctt ttccttttct tctccgccgt ttttccgcat
     7141 gggaagcccc cgccggcgtt gtgccggcgg gggcttcttt tgtccatcag ggcctgaaga
     7201 aaacgaaggg cctcaatggg acatttttct gcgcctcctc cacaggaggg gggccagtcc
     7261 tgccagggcc agggcggcgc ttccgggttc cggcacctgg gcgaaaagca tggcggaatt
     7321 ggcctgaaga tccatggaca tggccgcgtc gtacagaacg ccgtcgttca ggaatttgat
     7381 attgtccagg ctggcaggta tctctgtcag ggagagtgtg taattggttg ttcccgtaat
     7441 gtccacgatg accgcatctg cggtgagccc ctgaagcgtc agggaggcgt gttcccccag
     7501 aataagcgtg gtcaggtcag aggttccgtt caggataagg ctgcttcccg cctcaatggt
     7561 tacggtgagc gccccgccca gggaatccag tgaggtgcgg gtctggttga cggcggagat
     7621 atgggtgaag tcactcaggg aagccaggag gctgtccacg gttacatggt ccagcaccag
     7681 gctcctgtct ccgcttacgt tggttcctcc gctgatgttt ccggcgtatt tgtcaaagcc
     7741 gtaggccgtc gttcctgagg aaagattctg gatgcgtacg gtggagtttc cctccaccgt
     7801 tccgccggaa ccgccgccgg agatgctgcc ccaggagctc ccgttataaa gcgtggccgt
     7861 gtttccggtg atggtgacgg acgtgtcccc ctgaatggaa cccgccttgc ctccggcgta
     7921 gatgtcccct ttaaccaggc ctcctgtcaa gcaaaccgag cttcctgcca ggatggagcc
     7981 gccctggccc gcgccgtaaa cggaaccgct gataacgccg cctgtgacgg tgacgttggc
     8041 gccgccggaa atggagcctg tcaggccgcc gcccataaca tttccggtga cggagccgcc
     8101 gttgatgtaa acatgcgtgt ttcctccaat ggtggtggtt ccggttctgg catttgcgaa
     8161 gatgccgccc atgatttgat ggttgaaggt gccggagttg accacaatgt ccacattccc
     8221 ttggatatcc gtggcatagg agccgaccac agaggaggaa ttactgctgg taaaggtgcc
     8281 gaaggaggcg ttttccgcag agaattccag ataaacgttt ccggtgacgc cggcggcgtt
     8341 cacggctccg aagaccgtgc ttccgcctgt ggcggcatcc gtgaaacgga ggaaggtgtt
     8401 cccgttgagc gtgccggaag ctccgtgagc tccgtaccag gcggaggcct gtccctcctt
     8461 ggagatggtc agccagacgt ctcccgtgta gttgccgctg ttcgcataaa tatgagtggc
     8521 gtttccggag gtaatggaga cgtttacgga attccccgtt ccccctgcgg aggaatccgc
     8581 ctgggagcgg gcattaaagg taccggagct ggccagatta tcagcggttt tggtgaatcc
     8641 ctccgttttc cactcaatat tgccttccgg tgttccaccc tgggtgaagg agtcggaaga
     8701 aataaaagac ttttccgcgt taagcattcc ggaagtggca ggggcatagg aacccgtgcc
     8761 gtccagggcc aggtcgtgca gaacagcggt tccgctgccg ttgtattgaa tggtgaggcc
     8821 gttgcagccg gagccggagg taacggaaag cgcttcccca atcagcatgt ctcccagcca
     8881 gacgtaatag ccgcctttca gtccttccat atcattgccg cagatatatg ccatacgcag
     8941 gttttccgca ttggcggaca tgtccaggga atacagaatg gcatcgcccc atttgatgta
     9001 ggcctcatta atattgagcg tgccgtaaaa ggagctgttc cccaggctga tgctgaaatc
     9061 ggaggcagta tcccatccgt ctgctgcgcc gtttcccaga accaggttgc aatcaaaggt
     9121 gaagccgttt tgcagctccg tcccttccgc ccaggaatag gaaatggagc tttccccgga
     9181 ttgccccagg ctgatgccgt tctcgtccac agtgacattg gcgagggaaa accctttatt
     9241 ttccaactgg ctgttcgagc tccatgcggg ggattgcccg ccctggtgaa aattgatggc
     9301 caggccgccg gcgtcttttc tttcctgtcc ggcggtgcgc cccgcatagc ccatggccct
     9361 ggcgatattc ccggccataa gcagatctcc ctgggcattg ggatggaggc cgtcgctgcc
     9421 ggggttgttg aacatggagc tcacgccgta gaacggcgtg gaggatgtta cgtcaatgag
     9481 gccggcattg atgtcaatga ccgtgacgtt ctgcctgcca ttcccccatg ttttcagacg
     9541 atcattgtaa tcagccacgg cctggtgggt ggcgtccgaa ttgccgttgg aatgccgggt
     9601 ccagcaggga atggtaagga tgatgacatt tgcatgttca ttggcctgga acatggcctc
     9661 cacgatggtg tccatatcgt tcaggaggtt ttgggaaacg gagtcaaggc ggtccgccag
     9721 cgtggcgttg tttccgtcgg aaaggagatc gttggtgccg atcatcatga aaaaggtatc
     9781 cggcgtattg tccccggtga atgtctggcc tgaataggcg tttccgtttg ccttggtgcc
     9841 ggaaagcccc agccagttgt aaatgttgga gccgtcaaac ctgcttccgc tgttcctgcc
     9901 ggatatttcc caggccctgg cgcttgcctg ggaggaatgt tcattgttga atatctggcc
     9961 cccgtaggag gtccccgcag taacgccccc ggaataattg ccggttttga ctccttccgc
    10021 ctggtaggaa ataccgttgt ctgtaaatat tttatggagg gcccagcggt aggaggcgct
    10081 gttgaccccg tgggtgatgg agtcccccac gtacatgacg tttcccagtt ccgtgcccgc
    10141 cggggcggcg gttgctgcgg gaaatgccgc cgctagggca gccaggaggg ggagagggag
    10201 ccggattttc atcagagcgt caaggaaaaa tgaaaaggat atttgaaaaa gagttaagaa
    10261 aacgtccatc cctgaacgga gcaggaatgg acgcggtaag gaaaaagtca ggccctgcgg
    10321 cggcgcatca gaaagatacc cagccccagc aggccgagag aggccgcagc cggttccgga
    10381 acggcgctca gggcatattc cctcatctgg tcttctgtca aaacggccgt gtacaggtca
    10441 aaattattca annnnnnnnn ngtgtattct cccgtgattc cgttcggagc gctccatgtg
    10501 tttccggagg tgtctttggc ggaaaaattg atctgggtca tgttttggtt gctgaaggaa
    10561 gcgccgctgc tcaatgtggt cagcgtggtc agtgtggatg tgtcgttgcc cagcatggcg
    10621 gagatagaga cattccctgc attgaatgtg taaaacaggg tcagaggctt gctggtggaa
    10681 taatcggaaa acgtttgagc tttccagggg gaggaattag tgcctccgtt gtaagcaaaa
    10741 cctccggcgc acagggtgag atctcctgac tccgaaagtt gaaaccccaa cccttccaga
    10801 tttttgtaag agccggtatt ataggtaaac agcaatccta catttgactc tgcagtgctt
    10861 cccagaccgg aaatcgtgaa agccattgtc cagtgggcgg tgttgtttac gccaatagtc
    10921 gtgggggagg atacagtcaa cgcatcgccg gaggttccgt catagacggt ggaacctttt
    10981 gaataagccg cgtccaggcc gtcgatggaa gccccgaagg acaggctgct tcccatggcg
    11041 gcgatcagga gggcaagata tttagtcttc atgtcaattg gtagaaaagc ccttgcataa
    11101 agggattccg ctttttatcg gattcctaat ccgccaacaa ttaaaaatat tgatgataag
    11161 tgtgttattt gatgtaatgc attaactttc attagtattg ccgggtgctg gtcttttccg
    11221 gtccatttct tttgccatga ttttcctgcc ttgactcctg ttggaagggc aggatgccgc
    11281 aaacggatta ggaatccggc aggaggttga acatcaggga taaaaaagca ggagatgcct
    11341 tgccttattg gaaatgatgg aatatcggga gcaacggtca ttcattcctg aaaaacgaaa
    11401 tgaaattgtt ccgcggcctg ttgaccgtga gtgggcggaa attgaatcga ttccgacatg
    11461 gcctccgttt ccgcggggaa agaagagagg ggggcggctg gagaccttgc actttccgga
    11521 tgtgaggaag aacaggagaa agggcctctc cgtaggggat tccgttcccg gcccgcggtt
    11581 cctgcggccg ggtgaaggag gcccgcagtc tccggacggg acggggaagc agttggagaa
    11641 ccggcgtttt ccccagggcc ggaaaacaaa tcctccggca agccgtactt gccggaggat
    11701 gaaaaaacag tcaggaaaat tccctgtttt aaggggcgcg ttttactgcc tggcagggcc
    11761 gtccatgata gccttgtggc tcatattcca gccgccgccc agggccgtgt acagggaaac
    11821 cagcgtgctg gcatgctggt aataatactg tgaaagttga atctgagcgg ggtacaggtt
    11881 ctgctgggcg tacaatactt caatgtaatt ggaaaggccc gtgcggtagc gttcaaagga
    11941 gagtttcacc gccgtgtggt aggcttccac ggcctcggac tgggtggcgg tgatgctgcg
    12001 cagcttgtcc ctctggataa gcgtgctgga aacttccgcc agggcattaa gaaccgtttg
    12061 ttcataatcg tttctggccg ccaggaattc ggccttggcc gccttttcgg aagccgtcag
    12121 cttgcccgcc tggaagaggg ggccggtcag attggctccc aggccccagc cgccgcggcg
    12181 cccctgcacg tggcgcagat ccgcggaggc gaggccgccc gccgctgtca gggagatggt
    12241 cgggaagtag ttggcaatgg ccacgcccac ttcggcattg gcggcgcgca gctggtattc
    12301 gctctggcgg acgtcaggcc ttctggaaag gatgtaggcc ggaatgccgg cgggaacttt
    12361 gacgttatag gcgatgtccc gggtgctgcc ggaacggcgg atatggccgg gagcgcgtcc
    12421 ggccaggacg gagactgcat tttccagatt ggcaatttgc acctgaatgg cgggaatctg
    12481 ggcctgggag gaggccaggg ccgccttggc ggaagccacc tggagcctgt cgcctacctg
    12541 gccttcaagc tgttcatcaa acaggcgcag gctttcggaa taggattcca cggatttctg
    12601 cacgatggca agctgttcgt ccagctggag gagctggagg taggagtccg ccacctggcg
    12661 gagcagggaa agcatgagcg cgcgctggcc ttcttcggaa gccagataat tcgcgcgggc
    12721 cgcttccgtc atccggcgcg ttttgcccca gatatccagt tcccaggaaa tgccggcatc
    12781 aatcgctcct ggcgtcaatg tggttccggt agtctgggcg atgctgccgc cggtatagtt
    12841 ggagcctttg ctgacggagc cggaataatc cgcccacggg aagagcgggg cctccgtgat
    12901 ggtgatgtac tggcgtgcct tttccacgcg cgccatggta gctttcaaat cgcggttgtt
    12961 attgtaagtg tccgtcagga ggtcctgaag gtccttgttt ttgaaaactt tccaccaggg
    13021 aaggtccgca atggattccg tcgctgcagg ggctcccctg aatgccgcgg gcatgggcat
    13081 gtccaccggt ttgaagtcgg ggcccatcat gcaggaggag gcggcaaaag ccgttcccag
    13141 caggatggcg cgtgtgatgt atgggtttgt tcgcatatgc gtttagtctg tttttagatg
    13201 gttgcagggg cggttttacc cttttaatga ttcatgggct tcgtggaaca ggtgtttgcg
    13261 ggcgatgatt tcgtccgggt cttccgtttc caccgtcttc cgggcgatgc gtacgcggaa
    13321 gagcttcatg atgaagtaga aggagcacgg aatcaggaaa atgcccacga ccgtcgccat
    13381 cgtcatcccg gcaatgacca cgattccgat gatattgcgg gaataagcgc cggaaccgga
    13441 ggccagcgcc agcggaacgc agcccagaat gaaagcgaag gatgtcatca ggatggggcg
    13501 caggcggatt cttgcggcgg aaagcgtcgc ttccatcagc gttttgccgc gttccatttc
    13561 aatgacggcg aattccacaa tcagaatggc attcttggcc gccaggccga tgagcatcac
    13621 caggccgatt tgcgcgtaca ggttcaattc ataaccgaac cagtacaggc ccaggaacgc
    13681 gccgagggcc gcgatgggca ccgtcatgaa gatgctgagg ggcagggacc accgttcata
    13741 cagggccgcc agaatcagga agacaaagac ggaggagagc aggaaaatct gcacgatgcc
    13801 gataccgttc tggatttttt gttcctggta gctcatgtcc gcatagctgt agcccatgtc
    13861 ggaaggcatg ctggcttcaa aggttttttc cagcgcctcc atggcctggg agttggagta
    13921 gccctgggca ggcgtgacca tgagcttgga tgagttgtac aggttctggc gcagcaggaa
    13981 ttccggcccc ttgatgtccg tgattttcac gagggtggaa aggggtacgg agcttcccgt
    14041 gttgttcttc acgtagaagt ttttgagcat gtccgttccg gtgcggtcac tgccctgcgc
    14101 ctggatgtac acttgccact gctggccgta aagggtgatg tagttgatga acagggaacc
    14161 catgtaggcc tggagcaggc tgttggcttc actgatgctg acgcccagcg tggcgcattt
    14221 ttcttcatcc agccgcacat ctttctgttc cacggcgaag gacatcacgc tgcgcacgcc
    14281 gccgttgttg ttcgggtcga atatcggaag cttgttcgct tccttcacaa agatgtccgt
    14341 ttgttccgcc aggtattccg tgcccttgcc gtcgcggtct tccagcatga aggtgacgcc
    14401 gttggcggaa cctacccccg caatggccgg aggctggaag cacatggtga tgccttcgga
    14461 aactttcgtg gtcagcaggg cgttcagctg ggcggtgacc gcctgggcgc tctggtccgg
    14521 attggggcgt tcctcccagg gcttgagcat gacgaagaag aaggcgttgt tcgtgctctg
    14581 cacgcccgtc agcaggctga agccggagat ggcaatcacg tcttttacgt tcgggttctg
    14641 gcggatgagt tcggaaactt tgtccgcggc ggccgtagtc agctggaggg aacgggcttc
    14701 cggcatgatc agggccgcga acaggtagcc ctggtcttcg tccggcagga agccgttggg
    14761 gattttcttg gctacgggga caatcgcgta ggcgatcaga gccagcagcg gaatggagat
    14821 gatgagcttg cgggtcagga agtggcacac tttggtgtag ccgttggccg tggcgtcata
    14881 actgcggttg aaaacgcggt agaagaattt cagcgggccg cccttggtcg ggtccttggg
    14941 tttgagcaga atggcggaca gagccgggga gagcgtcagc gcgttgaacg cggaaatgag
    15001 catggaaatg gcgatggtca cggcaaactg ctggaagagc gtgcctgtaa tgcccggcag
    15061 cagcagggag ggcaggaaca ccgccgccag caccagggcg atggcgatga cggggccgga
    15121 tacttcctcc atggcggcga aagccgcctg gcggggcgtg aggccgcgct ccatgtggga
    15181 ttccacggct tccaccacca caatggcgtc atccaccacc aggccgatgg ccagcaccat
    15241 gcccatgagg cagatggtat tcaacgtgaa gcccaggagc ggaaacagcg cgaaggtggc
    15301 gatgatggaa accggaacgg cgatggccgg aatcagcgtg gcgcgccagc cttgcaggaa
    15361 gacgaacacc accagaacca ccagcaggag ggcttccacc agcgtgtgct caatttcctc
    15421 aatggaggcg cgcacggcca gcgtggtgtc cagcgtcagg ttgtattcca ttccggcggg
    15481 gaagctgcgg gagcggtctt caaagagctt gaccagggcg tccaccgtgt tgatggcgtt
    15541 ggagccgggg gcggcgtaca cggcgatggc gccggaatcc ctgccgttgt atttgctgga
    15601 tacgttgtaa gtctgggaac ccagctccac cttggcgatg tctttcaggt aaagaagctt
    15661 gttcccgtcc gcacggatga tgatgttgcc gaattcctca gccgtctgaa ggcgtccctt
    15721 ggtctggatg ctgtatgtga attccgttcc gggaggggcc ggctccgcgc cgatctggcc
    15781 gccgggaatc acggtgttct gcgcctggat ggcggattgg acttctccca cggagatgtt
    15841 ctgggcggcc attttggtgg tgtccagcca gatgcgcatg gcgtagcggc ctgcgccgaa
    15901 cacctgcacg tcgcccacgc cgggaacgcg tttcacgggg tccaccaggc tcacgtaggc
    15961 atagttgctc aggaagatgg cgttcaggct gtcatccgga gaatacaggt tgattacggc
    16021 cagcgggctg cctgtggact gcgtgatggt gatgcccatc tgggagacct ccgcgggaat
    16081 ctgggcggtg gcctgcccgt agcgcatgta ggccagagtc tggtccatgt tggggtccgt
    16141 gccaacttca aagacgatgc tcagggagca aatgccgttg ttggcgttgg tggaagtcat
    16201 gtaatccatc cccacgacgc cggaaatgga ctgctcaatg ggggaggcta cggaattggc
    16261 caccgtctcc gcattcgcgc ccgggtaggt cgcctgcatc ttgatggtgg gcgggacgat
    16321 gttcggatac tgctcaatgg gcagccccat cagggacagg ccgccgagca ggatcatcac
    16381 gatcgagatg acgatggcaa tggtcgggtg tttgatgaag aagctggaca tggcgttaaa
    16441 tcatgaaaat gcgcgggaaa agggaagatg gtgggtctcc ggataaggga ggttacttgg
    16501 aggacggctg ggcttctcca tccttcatgc cttctggcgt cctggccttt tcaaaggact
    16561 gggcccctat ggagggcatg acgggctggg aaactacggg cttgtaggga agcggcttga
    16621 cgggggattg tccctgcacc ttggccgcct gcatgattcc ctccaccacg atggggctgt
    16681 ccttcgtgaa ggtggagtgc ggcatgggaa tgatgttgat tacctgcatg ccgtcaacaa
    16741 cggggccgag gttgacgacc tgcatgtggg gaatgttctt gtcatccagg taaatgatga
    16801 atttggcgct ctgggcggac agaatggcac gcggaggcac cagcgcggcg ttttccagcg
    16861 ttttcaccgt ggcgcggacg cggacgaaca tgccgggccg cagcgtcagg ttcggattgg
    16921 gaatatgccc gacgatgttg atggtaccgg tctgggcatc cacattcctg tcaatggcca
    16981 cgggttcgcc ctgcatggga tattccgtgc cgttggcaag aatcacgtca aatttgaggt
    17041 gcttctgcct ggtcagatta ttgtccatgg agttcaggaa gtcccgttcg cttacggaga
    17101 aatccacccg aatggggtca atggaggaaa tggtggtcag cggcgtgggg ctggagggcg
    17161 tcagcaggtt gccgatggaa ggggttgcca ggccggccag gccggaaatg ggggctgtaa
    17221 tggtggtgaa tttaagattg atttttgctt catccacctg ggcctttgcc gtggcgatgg
    17281 cggcttccgt ttcctggacc tgctgcaggg cgtcgtccag ctgcttgcgg ctgacggagc
    17341 cggttttcac cagcggggta tagcgttcca cgtccagctt gtactttttc agcgtggctt
    17401 catattgttc cagtctccct tgggcctggt ccagcgttgc ctggaacggg cggggatcaa
    17461 tctggaaaag cacctggccc ttcttgacat aagcgccgtc ccggtaatcc ttggaaagaa
    17521 ggtatcccgt cacctgggag cggatttcgg catcttccgt gccgcgcagg gtgcccaccc
    17581 attcctgcct gatgggaaca tccttcgtga ccagtttggt cacctgcact tcgggaacga
    17641 gcgccggcgc ctgggctgcg gaatcctttt ccccttcgca ggaagttaat atcatcaccg
    17701 gggagcaaag gagcagggtt gttgcggcaa acgtcaaagg cttgttatac ataaatgaat
    17761 gcggttggtt gcggctggtg ccggaagatg cggcaaagcg gaagaatttt ctcttctttt
    17821 tcttgcaaag tcaacgcaaa gatgtgttct gaattctgca aaatatctgt aacctttgca
    17881 taatgaaggg ataatttaat taaacaaatg tataatatgt gacttgcccc tgtgccgcgc
    17941 ggaagaggct gctgcggttt ggggctcctt tatttcagga tgccagccgc ctcttgcgga
    18001 gcatggcgtc catgcgcggc tttcttccca tgaaatccat gaactgccgc atgggcggga
    18061 cagcatagcc tttttccaga atggttttcc ggaattttcc tgccgtttcc ctgtcctggc
    18121 ctccggcccg ttcaaaggct tcaaaaatat ctttgtccaa aacttccgcc cattggtagg
    18181 agaaataaaa ggaggcatag cccgcaggag aggaaaacaa gtgacgcgcc gtgcgcagct
    18241 tgccggctct tttgaaatct ttgaaataat ccatatctcc cgctacggcg gaatccacat
    18301 catcgagaga gccggcggag aagcgttccg gttccgaatg cacggccagg tccatctttg
    18361 cgtacaggag ttgcccggcc agcgcgaggg caggcgtatt gccgcgggag gcgtccagag
    18421 agttcagcat ttcctccgga atggggagtc ccgttcgttc atggcgcgca aagcttttca
    18481 gcacgtccgg ccgccagcac cagttttcca tgaattggga ggggacttcc acaaaatccc
    18541 gcggcacgct ggtccccgcc agggaaggaa tggaaaccct agtaaacatc aggtgcagca
    18601 agtggccgaa ctcatggaat aaagtcctga cttcccggtg ggacagtatc accgtgtctc
    18661 ctgccgcggg gggatggata t
//
LOCUS       DAWEMX010000040        18468 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1249_length_18468_cov_11.819964, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000040 DAWEMX010000000
VERSION     DAWEMX010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18468)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18468)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18468
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_18468_cov_11.819964"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12517..12565
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4021 a         4692 c         5508 g         4198 t
ORIGIN      
        1 atgaaaaatc agtctaaaat tgcattaggg acatggtcgt ggggcgcagg atttgccgga
       61 ggcgaccagg tattcggcaa taatctcgga gtagacgaac tgagacccgt attcgacgag
      121 gcgatggcaa acggggtgaa cttgtgggat tctgccgtgg tgtatggtat gggtacttca
      181 gaaacgttgc tctcgacatt tacaaagaat agaaagcggg aggatatatt tatctcgacc
      241 aagttcactc cgcagattgc aggcgagtcg gaagatccgg tagccgatat gctggcaggg
      301 agcctcgaac gtttcgccac cggttacgtc gatatttatt ggatacataa tccggccgac
      361 gtggaaagat ggacgcctta tcttattcca ctggtaaaaa gcggtaaggt aaaaagagtc
      421 ggtgtttcca accataacct cgcgcagatc aaacgtgtgg aggaaattct ctcgcaagaa
      481 gggatacatc tctctgccgt tcagaaccat tacagcctgc tctacctctc gtcagaaaag
      541 gcaggcattc tggattactg cagggagaac ggcatcgact tttgggctta catggtgctg
      601 gaacaggggg cgttgagcgg aaaatacgat accgcgcacc cgttgccagc cgatagccag
      661 cgtgggaaaa cctacaatcc gctgctgccg caaatagaaa aactcgttgc cgtgatgcgc
      721 accgtgggcg ataagtacga tattacgccg gcgcaggtgg ctctggcatg ggcgataacg
      781 aagggcacga cgccgattat tggtgtgacg aagccctcac aagtgcagga tgctgtccgt
      841 gccatgcaag tcgtgctgac tgttgacgaa gtgaagatgt tggaggatgc ggccgaaagc
      901 acgggggtgg atacgagagg ttcatgggaa aatccgatga tatgatgagg ctggttataa
      961 tcatcggagt tgtcctgtct gtatggtttg cggattgggc ggcggcacag cagattgtgg
     1021 atgtgcattg ccataacatt atgtctggct atatggaaat gttgaaaaaa catgatgccg
     1081 ctttggaaga gacttttccg ttgccggaat ggaatgtgga tgcccatctt gcctttatgg
     1141 aaaaagccgg aatcggttgt tctgtattgt cgatgcccgc accgcaaccc tacttcggtg
     1201 atactgggga gtgccgtcgc ataatccggg aatataacga gtattgcgcc gctctgaaaa
     1261 agaaatatcc gggcaagttc aaattttgcg ctgcactgcc attgcctgac gtgaacgctg
     1321 ccatagatga ggctgtttat gctctcgata cgctcggtgc agacggcatc aaactggcca
     1381 cgaacagtcg gggacagtat atcggggatg aagccctcga cccgttgatg gaggtattgc
     1441 acaagcggaa tgccgtcgtc atcattcatc cgcacaagcc taatccggtt aatgatacca
     1501 ttatttctac cgccccgctt gcagtctatg aatatccggc cgaaacgaca cgtgccgtcg
     1561 tcaatatgat ttcccgcaac gtgttggtgc gctatccgaa tctcaaggtg gtcgttcccc
     1621 attgcggctc gttcctgccg atggccattc cacgcatgaa ggctatacac ccggctatgc
     1681 aggcaaaggg atttatgggt tcaattgact gggatggcaa tctatcccgt ttgtactatg
     1741 atcttgccgg gggagcgagt cctgaaatcg tgaagatgat gctgacggtc gctccgccag
     1801 agcatatact ttatggttcg gattatcctt atatgtctga cggtgtgttg tttggaaatc
     1861 tgcaacggat gcgtgaacag tttgctgcgg ataaggaact gtccccctat ttggaacagt
     1921 ttttttcgga aaatgcccgt aatttattta acaaataata tttgaaaacg gagagcatac
     1981 ttttcggtag gataagataa cctgtgaaga gattgctaca gccgttactt ttctgataag
     2041 cgacgaagcc atatttgtaa ccggagctga ctgtgaaaac gaacggcatt ataggagcag
     2101 agaacaaata acagtaaaaa gaaagaatat gaaaaatata ttgattatca gcggccatac
     2161 aaaccttgcc gcatctgtag ccaataagac aatccttgaa accttgaacg aacggctgcc
     2221 ggaagctgaa atcgtaaagc ttgacgaact ttatcccgat tttaagatcg acgtggaagc
     2281 cgaacaacag aaattgctgc gggcagacat cattgtattg caattccctc tcttctggta
     2341 ctccgcaccc tccatattgg aacgctggat ggaagaaacc ttccgccacg gcttttcaca
     2401 cggcagtacc ggagacaagc tcaagggcaa gaaactcatt ctctctttca caacgggagc
     2461 acccgaaacg ctgtatagcc gtgaaggggc catgggctac gcgatcgatg aatttctgcc
     2521 atgctacaag gctgtctgcc gactgactca aatggaatac tgcggcagtg tttatacctg
     2581 cggcataagt tacggaaatc gtacgacacc ggaacttatc gagcagcaaa agggagcatc
     2641 ggtggaacac gctgaacgtc ttatccggtt acttgaaacg ctctgatgtt tgaataattt
     2701 cgacgatatg gccttcaatt gccctctatc cgatgactgc atgaatttca tgtatgcgga
     2761 ggcggcagga agcaaataag aacgcagagc ggccgggatt cctcattgga aatcccggcc
     2821 gacgtctgtt gaggatttaa gggagggttt attcctcagg cgcgatgttc ggctcgctgg
     2881 cgctgatgta gttcagcgga ttacagctgt ccggcctgcg gttgtctaaa agatcctgat
     2941 aaaatttttt cttgtatttc aacacttcca tttgtttttt caggattcgg agctgttccc
     3001 ggagactttt ctcctgcttg aagatcagtt cggcgcgctc cggaatggtg gaatcccctt
     3061 cctggcacag gtcgatgtag tgcttgacgc cttcaatcgg gaggcccgtc atccggaggc
     3121 agtggaccag gcggagccac cccagggtgt agtcggaaaa catacgggca ttccctccgc
     3181 tgcggtccac gtccgggatt aatccggcgt tttcgtaata ccggatggtg taggctgaca
     3241 tttccatcat ttccgcaacc tgcttgacag tgtatctggg ggtgcggtca taagaagggc
     3301 ggtaggtgcc ggatggattt ttctgtttaa tcatcgtgtg actgatagct tacagttact
     3361 ccaatatatg aggcgttgcc ggatgacaag ttttttactg tgctgcacgg gaataacgta
     3421 tttaaaggcc agggaaaacg gcggtaactc cattccgtgg aggaaggacc gggctgtgcg
     3481 tcggaatatc gggggaaggg gggcaaatga gttgactggc ttttcctccc cgttacaatg
     3541 aagcccgctc cggggacgga gcctgtaatg atgattattg cctccctttc cgattccgcc
     3601 cgttacgaaa tgttgaatcc cctgttcagg agagctttcg attttatcag ggagattgct
     3661 ccggcaaccc tggaaaccgg ccgccatgtc ctggaggaag acgccctggc cgtgatggtg
     3721 aatgaaccgg tgatgaagaa gcgggagaac gcccgcatgg aagtgcatga ccggtttatt
     3781 gacattcacg tgcccctttc ccgggaagag ggattcatgt ggaaagagcg cgcggcgctg
     3841 gaaaagcctt ccgaaccgtt caacagggaa aaggacgcgc agcattatga tgatgctccg
     3901 gacacctctt ttgtcgtgaa accggggcag ttcgccattt ttttcccgga ggatgcgcat
     3961 gccggctgca ttggggaagg aaagattctg aaactggtga ttaaagtcag aacggcctga
     4021 acctcattcc ggaaaaacag tccggaatgg agaacaggcc gtttcttcaa tttgcgggcg
     4081 ggagttgcgt tattttccgt cttccttccg ggcgtcctgt aaagcgtttt ccggaatcca
     4141 tacggccaca ttgccgccgt ggacaagaaa ccggccgttg ccttccccgt caatggagac
     4201 ggtttcctcc acgttgccgg tggcttcctt ccaagtttcc cctgcgtgct gcttgctcac
     4261 gttcatgact ttgtctccat cctctccatt ggaaatgagc acagccacgc cggaatgcgg
     4321 gtgctcttca tctcccacac gggtgaagcc gatggtgttc gggtggtcaa agtagtaatt
     4381 ttgttccccg aaagcatggt ttttacggat ttccagcaga ttgtcgatga tggcacggtg
     4441 catgggcggg ttgccgctca caccgtaata gtccccgtag aagatgcagg gataaccttc
     4501 cttcatgagc aggatgagcg cataggcgga gggtttgaac cagtcttcca cagctgattc
     4561 cagggaactg ccccactggg aatcatggtt gtccacaaag gtaacggcca gcgtagggtg
     4621 gttctgaacc agggttccgt ccagaatttt ggaaaggtcg taatcgcggc cctgcttgga
     4681 ggcctggtac atgttgtagt gcagggggac gtcaaacagg tccactttgt agcgttcctc
     4741 cttgaggtaa tcgtccaggg attccagatc ctgtttccag tattcaccca cggcgtagaa
     4801 gtctgctccc ctttccttgc ggacggctgc caggaatttc ctgatgaatt cgtcattgat
     4861 atgcttgatg gcgtccagcc tcatgccgtc cagatccagt tcccgggata cccatattcc
     4921 ccatctttcc atttctttca ccacttccgg gtggttgaag tccagattgg cgaacataag
     4981 gtagtcgtag ttgccgtttt cttcatccac gccttcgctc cagcttttgc cgtctcccat
     5041 aatcttgaaa atggaggttt tttccgtgcg tgcgtcatag tccgttccgg tgaagtggta
     5101 ccagtgccat ttgaagtcgg aatatttgtt tccgcggccg ggaaagttga agccggtcca
     5161 gccttcaatc tcgtaggcat ccgtaatttc cttgtcgcgg ttctgctggt ccacttcctt
     5221 ggccatgaat ttttccgtat agtccgcccc tgctttgtgg ttcatgacgg catccagata
     5281 aacgcctaca tggtgttcat gaagagcctt gatggcctcc tggagttcct gcctggtgcc
     5341 gtatttggtg cggacggtgt ttttctggtc gaattccccc aggtcaaaaa gatcataggt
     5401 gccgtatcct acgtcatttt gttccttccc tttgcaggct ggaggaatcc agacggccgt
     5461 aacacccatt tcctccaggt ggggggcgtc ttccttcaac ttgttccaaa actgtccgtc
     5521 gttgggcaga ttccactcaa aatactgcat catgattccg tttttcattt gatctgattc
     5581 cttgtcatac cgttcgggtt gtcgtgcttc caggggctgt ccggtggaaa aagcggtttt
     5641 cccgggcgag gttccggatg atgaatccat acgaagcccc caacagaata gagtcctgaa
     5701 agtacggcgc gttaggcata ctgccggtct tatgtcctgc aaaaatgcca gcggcaaaac
     5761 ggtaggcggg aagggttttt ctccgaagga tgggaaaaag cggtttcact cccccctgcc
     5821 gcaccccgga aagaacgggc cgggaacgag ggttcgtgta agtaagacag gtgggaagaa
     5881 agattatgcc tttcccgtgc cgggaaccag gtagtgcctg tttttcagcc ggtagtattt
     5941 caggaatcgg cggatgaaga gcagggtgac gataaaggaa agcacgtctg ccagaggaag
     6001 ggatgcccag actccgtcct gtccccagaa acggggcagg accagcagga acggcaggag
     6061 gaagagcatc tggcgcgtca gggacaggaa gattgccaga cgggcttttc caatggcctg
     6121 gaagaagttc ccgatgatga tctggctgcc gatgagcgga aaggccagca cgcagatgat
     6181 cagaccgttg gccgccaggc tgatgagcga ggcgtcatcc gtgaaaagcc cggcaatgcc
     6241 gcggggaaag aagacgcagg ccaggaatcc cagcgaggtg aaggatgtcc ctgccagaac
     6301 tccgtatttg agggtgagca gaacgcggcc cggcttcatg gctccgtggt tgtagccgat
     6361 gatgggctgc attccctgag tgaggccgat gacgagcatg gcgaacagaa taagaatgcg
     6421 gttgatgatg ccgtaggccc cgatggccag gtcccccccg tgatccgcaa gctgcctgtt
     6481 gatggcgacg gtgaccgcgc tgccgcagac gtttaccagg cagggagcca tcccgatgga
     6541 caggatgccg gtgacgatgg tcttgcgcag ctgccagatg ccgtggcgga agtggaccgt
     6601 gctggccgca tcggaaaagt gtaacagcac gcgcaccatg ccgacgacct gggcgagaag
     6661 ggtggccacg gctgctccgg taatgcccca tttgaaccag aagatgaaga tgggagccag
     6721 gatgatgttg attcctacgg aaagaagggt ggagcccatg gcttttttag ggtagccggt
     6781 agctctcatc acatgattga ggttgaagaa gacgtacgtg acgggcaggc ccagcaaggt
     6841 aatgagcatg aagtcgtacg cataggtgag ggtttgcccg ctggcgccga aggcggtcag
     6901 gatttccgta aggaaaagaa gcgtgggaat caccagcacc acagcggcaa tcagactcat
     6961 gatgaggacg ttgccaagcg tacgctcagt tccttcctgg tcttttcttc ccagccggat
     7021 ggagctgatg gccgccccgc cgatgcctac cagcgtgccc agggcgaaag tcaggttcat
     7081 gatggggaag gtgacggcca ggccggacag ggccagaggc cccacgccgt gaccgatgaa
     7141 cacgctgtca atgatgttgt acagggagta taccaccatg ccggcgatgg agggcaggga
     7201 gtactggatc agcagacgcc atacggatgc gttttccaat tctgcgatgt tgcgtgactg
     7261 ggctgccatg gacgttcctc ctttcaaaac aggtggcaaa ctataaaaac gcacggatgg
     7321 gttgtcaagc tgtccacctg tccggaaaac gacctttttc ccccagttcc agagagaggg
     7381 ctgtttttga ttgactgtgg aagcttttgg gggttgagtc ctttccgcct ttttcagaag
     7441 cacgccatga ccatgtccgt tttccccttc ctgcgtcttg tctccgtttg cggcgccgct
     7501 cttatgggtt cttcctgtct gcccatgttt ggcgccgggc aggtgctggc gggaagcagc
     7561 ctcgcggaag gtctgctgcc ctcttccact ctggagctgg tgtggagggg agacatccgt
     7621 tcttttccct cttccggttt gaggacgccc cgcgggttca aagtttctcc cgcggattct
     7681 tattccctgc tgatggaagc gcgcccgggg attgccggag agtgggcctg ctatcatgac
     7741 cgcagcaatt actactgggc ttttgtagcc ggaaagaagg aggaggaagc ggggctttac
     7801 gccgtgcgcc gcggcgtggc cgtaagcggc atttccggag aaattgaaga tgcccccgcc
     7861 gtgcaggatg ctcagccatt ctccgccatg taagcacagg ttctggtctt tttgcaaaaa
     7921 atgctgccgg aaccccgttt tccggcaagt tggacacgat aggtttgaca ggtagctgtt
     7981 attgcgggcg tattatgaac cgcatgaagt tccttttatt atcgtttgca tgggtgtgca
     8041 tggcttgcgc cggagcatgg gggcaggata cggccccgtc ttttccggct aacggggcca
     8101 attacaggct gtttccggcg gaccggcctc cgctggttcc caaaccccag cagctgcgct
     8161 gggacgacag ggccattccc gtgcagtccg tacgcatttt ggctccgtct ccgtccagga
     8221 cttcctatcc ggaacagatg aagttcattg tttccgaatt gaaatctttt ctggcggagc
     8281 accgcgtgaa ggtggctccg gacgggactt ttgccgttaa attcgtcaag ggggatgtga
     8341 aagccggcac ggaaaattcc aagctgaagg aggaggctta ttccctccgt gtaactcccg
     8401 gcggcgcact cattacggcg atggatacca gaggattcta ttacggcatg aaaacgctgg
     8461 agcagcttct tttgcgccgc ggcgggacga cgaccattgc cgcctgcgat atcgtggact
     8521 ggccggattt tgaaatccgc ggattcatga acgatgtggg acgcaattac atgccgctac
     8581 ctctgattgc acgggagctg gattccatgg cgcagctcaa gctgaatgtt taccacttcc
     8641 attttacgga gaaccccggc tggcggctgg aatccaaaat ttatccggag ctgaacgccc
     8701 ccgaaaatta tacgcgcatg cctggcaagt tttacacgca gaaggagttt aagcagctgg
     8761 tggagtactg ccgcctgcgc aatatcctgc tgattccgga gatggatatg ccggggcaca
     8821 gccagatgtt ccgcaaggcg ctcaacgtga agatgagcga tgaaaaagcc accgaagccc
     8881 tggtggccct gatcaaggag ttgtgttccc tggttcccaa ggagaaaatg cccatcatcc
     8941 acattggcac ggacgaggtg cgcggcaagg atgagcaggt gaacaatgag attcttaagg
     9001 agtacatcca tgcagtggag tcctgcggcc gcattcccat gagatggcag cccggcctga
     9061 cgccgaaggg ctataacggc tccatccagc agttatggtc cggccgccag aaccgtggcg
     9121 catggcctac cgacggagcg aagtatgttg attccctgga gacttacctg aaccaccttg
     9181 atccgtttga aacggccatg accatgtatt tccgccgggc atgcccgttt cggaatgcgg
     9241 aaggactggg catgatgctg tgttctttcc cggacctgga aattacggat ccgcgcaacc
     9301 aggttcttca gacgcccgtt tacgccggca tggcgttcgt ttccgaacct ttgtggaata
     9361 atccccatga gaaggtgctg ggagacccca accaggacga atatatgaag tatttttcca
     9421 atctgcccgt gcagggggat cttctgctga aggggtttgc ggattacgag aaccgcgtgc
     9481 tcgccatccg ggaccgtttt ttcgtggata aggagtttaa ttacgtacgg caggcgaata
     9541 ttccctggaa attgctgggg cctattccca acggcggtaa gacggaaaag gaattcgctc
     9601 cggaggagga caacaaggca gggaagatga gggattccta cgagattgac ggcgtcacct
     9661 atgagtggtc cggagacgat tacacggggg ccaccatcat tttcaagcat tactgcgatt
     9721 ttccgacgct gttcaatggc gcaaagatgg gagcttatcc ccacaaaaac cacacttatt
     9781 acgcgcagac ctggatttat tcccccaagg cgcagacggt gcctttctgg atcagcggac
     9841 atacctgggc cacgtccgat tggcgcaacg gtccggcgag cgttcccggc aagtggtttc
     9901 atgcggatcc caaatttttt gtgaacggcc gggagattgc ccccccgcaa tggaaaaagc
     9961 cgcgtaacag cggcgtgatg gtggatgaaa actaccattt ccgggagcct tccatggttc
    10021 ctcttaagaa gggttggaac cgcgtgctgg taaagagccc cagcaacaat tccgcgcgtc
    10081 ggtggatgtt cacattcgtt ccggtgctgg tgaaccccaa gacgcccggc tgcaatgtga
    10141 aggagtatcc cggcctcaaa ttttccacac gtccggaata gaaaatcccg tgatgagcat
    10201 ccggagctgg aatgagccgg ttgcagtatc cgccatcatc attaaaaaag aagccgcgct
    10261 tctggcaaaa gcgcggcttc tgattgcatg caaaggacac ggatacgaag ttaagcgctc
    10321 cggcggcgcg ttatcggagc ggcaagtccc ggcaggccaa gggaagccga gtccagttcc
    10381 ggcacgggaa gagccccttc attgtagatt caatggagca gggtaaccct gtatggtcac
    10441 agtattttcc agggaatatg ttccaatagt tcgtcaaagc cgagccccgt gcctccagga
    10501 acagctgcct gagggtccat gcctcccatc cggtctataa aggggtccgg ctcaaacagg
    10561 aggtgggtgc agaggccgca gaggggaatt tccgtgggca gggagttgcg gtaatattcc
    10621 gccgcctggt aaacggccca gagctgccct acggggtgat ccatgacagt ggtgaagacg
    10681 ggaggttcgt aaagatattc cggcgttgct tcccgtgcgg gtttgacgat gcggatgatg
    10741 ggcaggtccg aggagatggc cggctggtgt tccacatccg aacccatgtc cagggccaga
    10801 ggcatgccgg cgtctaccag acgttcccag ctctggatgg agaaggggca cgggtcttct
    10861 atgaagtcta ttctggtttt cagatggtgg ggcagggatt tccagaattt cagggcatcg
    10921 ttttcattca ggcatccgtt gaagtccagc cgccagcgcc aggagggaac catggatgcc
    10981 agcatcgtca gccgttccag ggcggcggcc agattggcgc tggccttaat ttttcctgcc
    11041 ttgaatccct tggattccat gatgcgaatg gtagcggggc tgacgcagga gggcagcgtg
    11101 gcgtggcttt ccggaatgtg aagaccgtca aacaggctga cgcccctcgc gcgtgcttcc
    11161 ccgtccgttt gggcgcactc catggcgcgg acgcccagag gcagagggcg gccttttctg
    11221 agcgcgtcca attcctcttg cagggtggcg tctcccagtt ccggccacgg ctggatgcag
    11281 ccgtacccgc cttcatccgt tttaatgagg gctcccttcc tgcacacttc cgcggctctg
    11341 gcgttcagag ggccgcaggc tgttaaagaa tattcatggt aaaacacaac tttaattcaa
    11401 acaacctttc cttcttttgt cacgatgaaa aaaggttttc attggatttt ggagggtgaa
    11461 acgtctaagc tctgcgtcat gtacagaaca ttggtgttcg gggcggacgg ccgggtggga
    11521 agccgtctgg cagcgtatct ggaagaacat ggcctggagg tggtgaagtg cggacggggc
    11581 ttttgtgatt tgaaggattt aagcgctttg aaacgtgttc tgctttcttc cggagccact
    11641 catgtggtga attgcgccgc tgtgagcgga ttggaagcct gcctggatga tccggagacg
    11701 gcccaccgcg tcaacgccat ggctccggag atgatggcgc gcatttgccg cctggagggc
    11761 atgcgcttta tccatttgag tacggattat gtgctggacg gcaggaggag aggtttgaaa
    11821 acggaggagg ggaagtgccg ccccgtaaat gtgtacggag agtccaagct ggaggcggaa
    11881 ttccgtgtgc gggatgagat gccggacgct ctgatagccc gcgtttcctg ggtattcggc
    11941 aatccggaac ggccctcatt cccggaaatg gtgctgcggc gggcaatgaa aagggagcct
    12001 ctggccgctg tggcggataa atggtccatg cctacatggt tggaagattt atgtgcatgg
    12061 ctccgttttc tggcttatga gagcggggcg tccggcgtgc tgcatctgtg tcagtccgga
    12121 gagcctgttt cctggcacag ttatgccgtg gccgtgctga aatgcgccgt gaagcacggg
    12181 ttactgcctt ctttgccgcc tgtggcggaa caaaagctcg acgaacaggc cggtttcagg
    12241 gatgctcggc cccggcatac cgccatgtcc agcgaacgcc tctcttccct gatgaagcag
    12301 cccgtgaggg cgtatgaaga ggccattgac atggccgtgg cccgctatgc cgcagatcca
    12361 acctttctct ccagcatttc atgaatgatt tccacaattc ccgtgattcc cgccccaggc
    12421 ccgtttaccg ggaccgccgt tcccagccgg gccgcccgcg cacagctccc aaaaaaaacc
    12481 tttttgccaa caagtcgccg gaagggagcg aacaatnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnctgga atgagtccag ccgcacgcct ctgcgcatgg
    12601 tgtaccacgg gaaggacgtt tttgccgaac gtgacctgga tgctgcgctg gaacgcgccg
    12661 tgaagctgcg cagggaagtt ctgcgcctag atgaaaccac taatgcgtac cgtgtgctgc
    12721 acggagattc cgacggactg ggcggcctgg tggtggaccg ttacgcggac gtgctgagcc
    12781 tggaggtgag tacgctggcc gtctggcaga ggctgaaccg ctggctgccc ctgctgcacc
    12841 gcctgtgcgg cacaaaacgc catgtggtgc aggtggatga gggcattgcc cgcatggagg
    12901 gtatccgggc ggaggaggcg ccggcatccc cggctcccgt caggctggtg aagattgtgg
    12961 aaaacggcat cacctatgaa gtggactttg cccaggggca caagacgggc tttttctgtg
    13021 accagcggga caaccgcctc aagttcgcct ccctggtgaa gggggccacg gtgctggacc
    13081 tgtgctgcta cagcggcggc ttttccattg ccgccaagat gctgggcgga gcggcggagg
    13141 taacggctgt ggatttggat gagaaagccg ttgccatggc taaaagaaac ggaaatatca
    13201 accatcagcg catcgacttc gtgcatgcgg atgcctttgt ctatgcgcgc cagatggtgc
    13261 gcaacggccg cctgtttgac gctgtgctgc tggacccgcc caaattcatt gttggcaggg
    13321 acggatatga agagggcatc aaaaaatacc atgacctcaa tatgttgggg ctgcaatgcg
    13381 tgcgtcccgg cggcctgttt gtcacctgtt cctgttccgg gctgctttcc cccgcggagt
    13441 ttgagcatac cgtcatcaag gccgcccagc ggcaggggcg caagcttcag attatggcga
    13501 tgaccggccc cggctgggac caccctttcc tgagcactta tccggagggc cgttatctga
    13561 aggttctgtg ggcaattgcc ctgtagctgg aggttgaggc ccgggatgca tttcggaaaa
    13621 acctttcagg cgtgttgacg ttctttgcca ctctctgctt tcgtggtgag gaatatggca
    13681 aaacaagtat tgcagcggct cagttttacg gccggggagc tgaccccgtg gctggccgga
    13741 cgcgccgacc tggatcccgt gtcccgtggt gcctcccggc tgataaattt tctggtcagc
    13801 cccttcggcg gtctccggcg caggccggga acacgcctgg tcgcccgcgc gggatgccgg
    13861 gagggcatgg tgaggctggt ttccttcaag tactccacgg gcgtgcagtt catgctggaa
    13921 gtggggcgcg gctacgtccg gtattttaaa aatggcgcgc ttctgacgga tacggaaggc
    13981 ggggtgctgg aaacgcttac tccgtggaag acggatgaac aggtaagcaa cctccggatg
    14041 cagcagctga atgacgtgat ttactgcgtg gaaccttcta cgccgcctat gacgctggcc
    14101 cgctacgcag acgatgactg gcagctggaa gcgttggagt tttccggaat tccttatgag
    14161 agttccctgt tgaatgccgt gcggctggaa tgccgcatgg tgcgggaggg gagcgtcaac
    14221 aggctgctgg cgacggcgga cgatgatgta ttcactccgg aaatggaggg aaaggagttt
    14281 ctgcgcatca ccaggaaata tggagaaacc gtggcggagg gaaaccagat gcctttttac
    14341 catttgacga ctctgagccg ggatctttac aagggagaga ctttttccat gaaccgggag
    14401 gacggctggc ggcaggctta tacctgcatc cgcgatttca gccgggaaag cgactaccag
    14461 gaaggggtgg accgcccgga gaggtacacg gccttttttg aaaaaggggc cgatgccagt
    14521 acccgcattt atgtcaacgg cgcgtggacg ttggagacga cggggacctg ggatgcggaa
    14581 tgggaaatct gccgggggta tccggacggc tccaattacc ttcccaaccg tccggaatta
    14641 gtgtggcatt ccgtgaagag cttccagcag agggaggggt tccggaataa tttcaccctg
    14701 agcggcaatg aagaggaaat gtcctactac aaaatccgcc tcatggctta caaggacggg
    14761 tcttctgcgg gaacgcccgt attccgcgca tcagccggct cctttaacca tgaagtggtg
    14821 gtggaggaat atgtttcccc gcgcagcgcc tatcttgcga gtgcccttca tctgtcctat
    14881 catacgctga gtgactgtga taccaatgac tggtcttttg gtgcgttcgg ggtccggaac
    14941 ggttatccgt gcacggtaga gttccatcag gggcggctgt ggttcggagg cactcctggg
    15001 cagccccaga cgctgtgggc cagcagggtg gatgattttt ccgcgtttac gcccggcatt
    15061 ccggcggatt cccccatgat tctgacaatg gccgcttccc agcagaaccg catttcctgg
    15121 atagcttccc tccgggggct gatgattgga accagcgagg gggaatggcg tctgtccgcc
    15181 accaattcag aagggttgaa tgcctctaat gccggatttg agcgccattc cggcgtgggg
    15241 tctgcctccc tggatgccct gtctgtagaa aacagccttc tttttgtgca gcagggcggg
    15301 atgaaggttc gggaattatt ttattctctg gaggccgacg gttaccagac aagggatgtg
    15361 agcctgttgt ccgaccattt gctgggggag ggaattgtgg actggactgt acagaggagc
    15421 acggcgttcc atgtctggtg cgttctgggt gacggcagcg ccgtatgcat gactctgaac
    15481 agggaacaga atgtggtggc gtggcatgca caccgcctgg aacacggccg cattctgtcc
    15541 gtggcgtccc tgcgcggttc ccgcaataca ccggatgagg aggtatggtt tgccgtggcg
    15601 cgcggggaag gggaggaggc ctgcattacg gtggaatgca tggcggatgg gaatgactgc
    15661 ctggatgcct gcacggaggc tgtcgtgaag ggggagacgc tgtccggcct gagccatttg
    15721 gcgggatgcg gcgcctttct ggtgggcggg gacggcgcat gcatggacat cagcgttgac
    15781 ggagccggga atgccgcctg tcccggaagg ggggatgggg aaacagtatc cgtaggcctg
    15841 gccgctccgg cggaagtaag gacgatgccc ctggaaacgc ttgagacgct gggttgcttt
    15901 aaaaagcaac tcggtgcgcg cgtcctgctt catgaaagca ccctgaaatt ccgctacgga
    15961 acgggaactc cggaggcatg gaaggatttc cagcccggca ggtacagtgt ttccgaacct
    16021 tactccggct atgtgcggat gattcacaac tatggtatgg atgagcagtc ttcctttgcc
    16081 ctccgtgtgg agactccggc tccatttaac cttctggccc tggtgctgga tgtggaatta
    16141 taggcgcagg agtgcgttta cggctgttca gccgcttctt catgggaaag catgtgtaca
    16201 atgaccttga tgccgtgttt ttccccagcc gccttcgcca gggaacgaat ggcagaggcc
    16261 gtcattccat tgcggccgat gatgcgtgcc acgtccgttt tttccagaat caggcggaag
    16321 cggacggcgt ctccctgcgg ggattccgca acgcgcaatt ccgcctgttc cgggtgttgc
    16381 acgaattgca gggcgatcaa ttgcaggtac tggcggattt gttctacggc aggatgcatg
    16441 aagggaaata tgggttattt tcatgaacca tactataaag ccgcctgatt gaaaagatgc
    16501 aattccagac attccctgaa aaagaaagtc gccccggccg taaaacggcc ggggcgcccc
    16561 aactacccta tgaaactgga tcggttaaga tccagagagc ttttttaacg agcgctcacc
    16621 tgctcgaggc gcaatatagt ggaggaaaag gggatacgca agtatttttt cattggaacg
    16681 atgtttttgt tcaatggaaa tagcatcgta tttttacttc attttgacaa tatcgggacc
    16741 ggaggaatgg ttattgttat tgtcatgaaa tcatcttccg ggaagcaatt catacgtgaa
    16801 ccccttcagg tattccgttt ccggaatatt caataaaacc ggatgatccg ccctttggcc
    16861 gtgttgctgc atcagacgca gggtggccgg agcatcaatg gcggcgtcaa gcacgctctc
    16921 ccggaacagg tccgctccgg cgtggtggga acagcagaag gtggaaagga tgcctcccgg
    16981 ggccagaagc ttcatggcgc ggaggtggat ttccttatat ccgcgcatgg cgtcatgcac
    17041 ggattttttg tttctggtaa aagagggcgg atccaggata attaaatccc atttgtgttt
    17101 tcctccgtcc cggacaaggg ccgcttcctt tttcaggaag tcaaacgcgt tatccgtgac
    17161 acactgcacg gagactccgt tcaggcgggc gttccgcgct acggcgtcca tagcatcctg
    17221 ggaaacgtcc acggccgtta cctccgaggc accggcaagg gcgcaggcca gggcgaaacc
    17281 gccctgattg cagaagcagt ccagcacgcg gcgtccgcgg gcgaagcgag ccacggcggc
    17341 ataattgtca agctggtcca gatacaggcc ggttttctgt cccgtctgaa ggtcaatcat
    17401 gaattgcacg ctgccgctgc gtgcggcaaa aggttccggt tgctgtcctc gtgccacccg
    17461 gacggagggg gaaatacctt ctgccgccag catgggtgaa tcattcctga caataatccc
    17521 gcggggagaa agaaggtctt ccaaaacatt caggatgatg gggaggcggc attccatggc
    17581 gatcgtcagc gtctggacga ccagataatc cgcgtaacgg tccacaatga ggccgggaag
    17641 cccgtcggat tcgctccaaa cgagccgggt gagagtttct tccggaagcc ggcggcggcg
    17701 cagttctact gcttggctga tgcgcctgga aaaaaaatct ccgttcagtt cctgtttgcg
    17761 gcgggaaaag cgcctggcga cgatctggga atggggattg aacatggcgg aacccagaaa
    17821 gcggtccttg aaatccttca gggccacgac gtcccccggc tgcggattgc cgaaaatttt
    17881 gcgcacttcc gtgccgtaaa cccagtcgtg cccttgaaaa atgcgtgctc ttggagaaat
    17941 aatcaaacct gccatgtcag gaaaggcaat acatgatcgg ggtggaaagt tcaagggtaa
    18001 tgcgggcggc tcccagaaag gccgcggaaa gggtggaggc ctccgttccc tgggattttt
    18061 cgcgcaagta cgccgctgtg catcattccg ggactatttc ggttgaaaat gcccggaaac
    18121 gaagtaaaat agggcgcgtt atgaaagtag aaacttttga aactccccag gatgctgcca
    18181 aggctttggc aggagaagtt gcggaattga tccgtacgcg cgccgctgaa ggcaaaaacg
    18241 tggttcttgg ccttgccaca ggcgccactc cgctgccttt ttacgcagag ctggtccgca
    18301 tgcataagga ggaaggcctt tcctttgcca acgtgatttc cttcaatctg gacgaataca
    18361 gcggcttgga tcgcgaccat ccggaatcct actggtactt catgcacacg aatcttttca
    18421 accacatcga catcaagccg gaaaacatca acctgccctc cggcacgg
//
LOCUS       DAWEMX010000041        18325 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1257_length_18325_cov_11.732950, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000041 DAWEMX010000000
VERSION     DAWEMX010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18325)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18325)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18325
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_18325_cov_11.732950"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16086..16095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3615 a         5098 c         5517 g         4085 t
ORIGIN      
        1 tggggagggt tccgtttttc ctggtgctgg cagggtactt cctgggcagg aacatcacct
       61 ggaacaaggc ttttaaccgg gctttctggc tgtttattcc gttcatgttc tggaatgtac
      121 tgtacgtgtt ccttgtcctg cctcatgacg gcgcttcctt ccgccttgag aatttaattg
      181 gtatccggga cgtctttctt ccgggcatga accttttctc cgcagatgac tcccatgccg
      241 ttccccccat cggcccttcc tggttcctga gggacattat cattctcacg ttgctgacgc
      301 ctcttctggt ccgcgtcaaa atcctgctgt ttcctgcggt gctcctcttt ttctgtttct
      361 tcaacacggc cccggaccac acggagacga tttccattgg aacctgtgct ttttacctgc
      421 tgggcgttgc gctcagttcc cggcggattg acgacattca tctgttgatg aataaaaaat
      481 tcggcatttt tttctgggta agcattttta tgcccgttgt actgatagcg ctgtattccg
      541 ccggagtgat ccccctgtgg aaagagacgg ccattggcat gcttctgggt gtcatgatga
      601 tcatgtacgc aggcatctgg atggaaaagc acctgcccgg cctgtctgcg aaaatagctt
      661 tgctggctcc cgcctgcttt ctgacgttca tgctgcactg gcccatttac ggtttcctgc
      721 ctcccgccat gaaatccagc ctgttcgcct tcttcacgcc gttgctggtg tttgcggcca
      781 tcgtcctgtt ttattttgcc ctgaaacgtt ttgccccgtt ccttctgccg tatctggccc
      841 atgtgaaaaa taccggccgg cggcaagcgc agcatggcgc gtgcgacgaa acgattcctt
      901 taatcagggg cctttcaggg atgagtcaat catcagaaag ggacgccggg aaccccatgc
      961 ccggaacacg ggagagcttt taaagaacca cgggcaaacg ggttccagga agccgtagat
     1021 tgccaggtga aaggactccc ggtacatccg ttgctcttct tccggctcag gaaggtaaat
     1081 gaaagaattt ggctggatgg aatccagcac aggcatcccc cacagttgaa gggagaatct
     1141 tttgatttca aaccggaggc gcatgttccc ccggaaaaca actgccgcct ccacctggcc
     1201 cgcagggttt gccttcatga aagcaaacgc tttttcccat gccccgggca gaagttggca
     1261 ggagattgtt ccaccctggt cgcgttccac ctcctgaatg tcctccggcc caatgaggga
     1321 ttccgggcgg atgaagacat aggtccggac ggggttcggg aaatcggggc aggtataggg
     1381 gatggaacgg aatccgcttt tcaggcggat ggattttccc tgcagaagtt ccggaagaaa
     1441 atgctgttcc aattcccggc tgcgcggatg gatgggataa agagccacgt tttccctcac
     1501 atgccgtttt tgctgttccg tcagcggcag aagggtgttg ttgatggccc ggcacagggc
     1561 atccagccgg ctgtcatccc ccagaccggg aataagagtg gtgcgggaag gcattccctg
     1621 gaataagggg gtggacagaa tggttccgcg cagaacgaca gcaatcctgt ccttgccggg
     1681 gcgtagtccg gccgatgccc gctccagttt acggaatgct tcttcgttcc aggtgatgtc
     1741 caggcttccc ggatgttcca tatctggctc cgcatgtctg gcgcagtctc cggaaatgat
     1801 gggagaattc cccaggaaca gatattcttc ctctcccgtt ttttcctgcc ggtgcgccgg
     1861 gagcttccag agcctgaagc ccggtacgcg gaggactcct tcttccacca gacggcggct
     1921 ttccggatgg acggcgtaca gggccagatc ctgtggaatg aagggtgtgt ttttttctgt
     1981 gggtgcctgc cggttgagca gttccgccag tccggaggct tcattttttc cgtccagtcc
     2041 ggagatttca aaagtacggg atatgacggc ctgcaccacg ggggccgact tgatttttcc
     2101 ctgaacgacg agggccagcc tgtcgcggcc cagattcatg gcggaggttg ccgctttcat
     2161 ggtttttccg ccctgtgtat tcagggtgat gtggaggcat tctcttctct ccggctcaat
     2221 tcttgcctgt tccacttccg attccgtgat gatgggggta tttttcagga acagccgttc
     2281 ccgctgcatt tttccatggc ggtcgcggaa ttcgtatgta taaggcgtgt atccttccgg
     2341 aacagggctc ctttcccggt ccagctcctg gctgtccgga tgcaccatcc tgacggagac
     2401 ggcgggaaca gacggcggaa ccgctttcgc gcgggccgaa agcgggaaac aaaaggcggg
     2461 aaaaagcagt agataaaata attgactcac agtcaacgga aatagaactt ttggagaacg
     2521 gcaagcaaga gaaaagttga ttcggcgcac aacaaaggag aacggtgttc ccggcgcctg
     2581 gggaagccct ccgcggaaca gcacgctggc ggaagccggc aggcaggcgg gacatcagcg
     2641 ggatgccatg ccctccagga tttattgccg cggcttgcgg cgggcagaag agggcgggga
     2701 tgatgaaggg ccggaacgtc tgggcgctcc gtattcctcc ggtacttccg ccgccaggga
     2761 tacatcttcc ggaaggctga tttcgtcgtc gtaataattc ttcatcctct tctgggcgtc
     2821 cttgatcagg gtggcttccg cccattcctc caccgtcatg ttgtcccttc tggcggcttc
     2881 ttcgatgagt ttgtaatcct cggaactcag catgacgaca aagggcttcc aattggctct
     2941 tgccgcctcc ttcagttttt tctccctttc cgttttttcc atcatgagcc ggatgagagc
     3001 ttccttgtct ttggggatgg ccaccttggc aagccagttg cggaccgtgt tgggagacac
     3061 gaagcattcc gccgcaaaat cattgcggga aatgttctgc tgtttcagcc aagccttgat
     3121 ctgcactttg agttcgtcct gattctcact catgcaccct ttgtaacgaa aatggtgcta
     3181 tttttcaacc ttttttgcat aaagtcattt taatggtata tttttgttga ctgaaatacc
     3241 gtttcaggtg cattgtgggg gtgcaagagg gctgttaagg ccgttattgc accaaaaaaa
     3301 ggatttttat gaagacggaa aataaaaaaa agagtcagaa aaaaattcaa atcaagggga
     3361 aaatgccgtt atcggtccat tttttccggg atgcggaagc ggacgcgctg aacatggaac
     3421 gctgccagct ggataaagcc accctgattg cctgtgccgt caaacgcctc cgggaagatt
     3481 tggggaacgt tcccgattcc ggtaaaaaac cttccgcatg actcaggaga tttactggga
     3541 acttctggcc tggctgttcc gttcctttct ggcggtgact gcgttcgcct ttgtcgtggc
     3601 cgggtaccag accctgcgca gccgcccgcg caggggccgc agccgtaaaa aataggaggg
     3661 cagggggccg gtttaccgtc cgtcacggaa tgccgtcccg ctttttaagg gaaatgtttt
     3721 tgaccttatg gggcattgaa gaattccgag gctgtgctcc ttcatatttg ggcggtccgt
     3781 catgaaaagc aggcggaaat ctgccggaga aagtggacgg ctgcctttct ccggtagaag
     3841 ggcgttgcag gaagaatgga aaaattccgg aaaaggtttc cacttttccg ccagcgcaga
     3901 ttctctaaac ggctgtcatc ttcccagggg ccggccggat ggaaatccgt attgccagaa
     3961 acggggaatg gcggtaacat tccgccgcta tgagtattga gagtttcgca aatcagcatg
     4021 tgctggagtt ggtggcttat cagccgggca agccgattga ggagacggcc cgggaactgg
     4081 gcctgaatcc ccatgatatt gtgaaactgg cgtctaatga aaatcctctg gggccgtccc
     4141 cgaaggcggt ggaggcgatt gcccgtgcgg ctgccggcgt gaacatttat ccggacggag
     4201 cggctttccg cctgcgttcc gctattgcgg agttctgcgg cgtggagttc ggccagacgg
     4261 tagtgggcac gggcagcagc gaagtgattg aactgatctg ccatgccctg ctgaatcccc
     4321 gtgcggaggt ggtggccgcc aaacacgcct tttccatgta ccctatcatg tccaagctgt
     4381 ttggcgccgc gtacgtggaa gtgcccaaca aggaggactg gacgcatgac ctggacggct
     4441 tcctggccgc tattacggag aatacgcgcg tcgtgttcat tacgaatccc accaatcccg
     4501 taggcaccgt agtgggacag caggaaatag acgatttcat ggcgaaggtc ccggaacatg
     4561 tgctggtggt ctttgacgag gcgtaccggg agttttccga caatcctccg gataccctca
     4621 aatttgtgcg agaaggccgc aacgtggttg ttctgcgcac cttctccaag gcttacggcc
     4681 tggcggggct gcgcgtgggc tacggcattg cgccggaacc ggtttgcagc atgctgcaca
     4741 aggcgcgtgc tccgttcaac ctgcatgttc tggcccagga ggccgccctg gccgccctgg
     4801 aggaccggga gcatgtgcgc cgcactgtgg agaataataa ggaaggcatg cgtttttatg
     4861 agcaggcttt ccgggaaatg ggcctggaat ggattcccag ccagggcaac tttatcctgg
     4921 tgaaagtggg ccggggcaag caggtgttcc aggatatgct tgccagggga gtcatcgtcc
     4981 gcgcgcagga cggttacggc ctgccggaat ggatacgcat cagcgtgggc actcctgcgg
     5041 agaacgcccg ctgcattgag gtattgaaag aggttcttta aacagttttt ccgtttttcg
     5101 tgtctcaccc tgcatgatga tctccccgga acagtatatt gacggtctgg aatggcgtta
     5161 cgcctgcaaa aaatttgatc ctgaagcccg tcttgaacag cccacctggc atgcgctggc
     5221 ggaaagcctg cgtctcagtc cctcctccct ggggcttcag ctctggaagt ttgtcgtcgt
     5281 gaccaacagg gaactgaagg ctcgcctgcg ggaggtttcc tggaaccagt cccaggtgga
     5341 ggactgttcc cattacgtgg tgctgtgctc ccggcgcgac gccaccaggc gggatgtgga
     5401 ccgttacctg gcccagattg aattcacgcg ccgaccctcc gctgaaaaac tggcttcctc
     5461 cgccgatttt tataccagct acgtggaggc tctggctccg gaaaagatgc atgcctggct
     5521 ggattgccag gtgtacattg ccgtggggtt cctgctcagc gccgccgccg ctctgcgggt
     5581 ggattcctgc acgatcggcg gcatggaccg cgccaagtat gatgaaatcc tgggcctgga
     5641 cggtacgccg tatcgctctg tggtgggggt ggctctgggc taccgcgcca aggatgacgc
     5701 ttatgcccgc gaggccaagg tgcgctttcc cgccggagag gtgattgata tccgcgcctg
     5761 attttatttt ttgtccggaa ccggccgccc taatagggac ggccggttcc cgttttttgc
     5821 cgtggggggg cgttttgccg tcgtgcggca tcttttttcc ctgcagcccg gggctccacg
     5881 ttttttatct cgccaacaac gggttctttg ggataataat ttattggaac aggagggaaa
     5941 gcctctcttg gccggcggtt ttccttcatg aagcccggcg cgcggcaatc cgtttgggcg
     6001 gaatgtccgt cccgcccggt tgagagaggg agaattcttc cgcttttctc cctttctctg
     6061 caagaaaggg gcggcgggcg cgtagttttt ggagcaaatc agacatttcc ttttcctaaa
     6121 caagactaaa acatgaagaa aaacctattc ctgatgatcg ccgttcttgc cgcgtctccc
     6181 gtgatggggc aggacgccaa gcagattgcg gattcccttt ccatccctcc ggtgaaagcc
     6241 ggagcgaagc aactgcccat gccgagcgtt tccggagccc agattaaatt gctgggggcc
     6301 gactatgagc agctcgtcaa cagcaaaggc aagatagccc ctgttatttc agataccccc
     6361 gtcaacgtca gcttcaaggt gaccaaggac ggcaaggaag ccgtcagcaa ggattatgaa
     6421 atcatgctgc aggctcctca ggccgcccag ggcaacccca agccgcggat tatcccggaa
     6481 atcctgcaat ggaagggggg acagggagaa tataagctgg ggaatacggt caccatagca
     6541 tgcccggaca aggagctggg caaattgttt gcggcggata tggaagacgt gctggggaag
     6601 aaggtcaagc ttgtcgcccc cggggcgaag gcggatattt ccctttccct gttgaagggc
     6661 ggcaatctgg gcagggaagg ataccggctc cagattgcca gggacggcgt ccgccttggt
     6721 gcggcggctc ccaccggcct gttctggggc acgcgcaccc tgctgcagat gctccgccag
     6781 acgcccggca gcgtgccctg cggcacggcc gtggattttc cgcgctacca gctccgcggg
     6841 ttcatgcttg atgtcgcgcg cacgccttac cccctcagtt acctgaagga cgtgatccgc
     6901 accatggcct ggtacaagat gaatgacctg cacctggtca tcaacaacaa ctatattttc
     6961 cacgaacact atgtggacaa cggccatgat ccgttcaagg aaagctatgc ggctttccgc
     7021 ctggaatcca aaatgaaggg aaaggacgga acgcccctga cagccaagga tcttttttat
     7081 accaagaagg aatttgccga cctggtcagc tatgccagaa agtacggcgt caatattgtt
     7141 ccggagttcg acacgccagg gcatgccctt tcctttacgc gcctgcggcc cgatctcatt
     7201 tataaagggc ccatgaatca tgagaagcgc cgctgcgaga tgctggacgc cgccaacccg
     7261 gaaacgatcg acctggtgag caaggttttt gacgaataca tgctgaagga tcccaagctg
     7321 ggccgtcccg tgttcgcgga ctgcggcgtg gtgcatgtgg gggcggatga attttatggg
     7381 gacaaggaag attaccgcca ttttgcaaac gccgtgctca gccatgccct caagcgcggt
     7441 tatacgcccc gcatctgggg aagcctcagc gccaagccgg gcaagacccc ggtagtcagc
     7501 aaaggggtcc agatgaacct ttggagcacc ggctggatga aagcatggga agccgtcaac
     7561 cagggctatg atgtgatcaa tacgaacgat ggagcccttt atatcgttcc ctttgccggc
     7621 tactaccgga tggacagaaa ccataagggg ctgtacaata actggattcc caaccgcatc
     7681 ggcaatgaaa cgctgccttc cgggcatccc cagctcctcg gaggcacctt cgccgtctgg
     7741 aatgatgaaa cggacattat gcatacgggg tacgcgcctt atgacatttg ggggatcatt
     7801 tccggttcca tggacgtgct cagccagaaa ctgtggggaa ccgccaaggc tccggatacg
     7861 tttgaacagc atcgcgagct ggtttcctcc atcggcaacg ctccgcgcac caatcccctg
     7921 cataaatgga aagacagcca gccctttacg gtgaagcctt cctcccttcc ccagaagctg
     7981 gacaaacctg ccctggggcc gaattaccgc ctgaccatgg aactggaatt gactgccgct
     8041 ccggaaggga aggagcaggt gctgcttgcc gcgcctgagg gcgaattgct tgcggtcatg
     8101 aaggacggca ccgtcggatt ccgccgtgac gattctctgg aattttcctt cggcgccaag
     8161 ctgcccgtag gcaagaaggt caaggtggaa attgtgggag aaccggagaa aaccagcctg
     8221 ttgcttgacg gggaacctgc cggaactgcc gtgctgaaga atttttccga taagtccaag
     8281 gatttttcgg acaaattcaa gcatcgtccc aaagttcacc gttccacgtt cattctgcct
     8341 ctgaaggaac ttggctcctc cttccagggg aaggtgttcc atatgaacgt acagccgttg
     8401 taattcccgg catttggcca ggccgccgcc tttgacgggg cggcctgcag cccggcatcc
     8461 ctgccgcatg ggatgccggg tttcttttta tggaaggggg gagaggaaaa cttccggaat
     8521 gtttcccgat gtcttccccc ttattcctgt tccgggaaag aaagaggggc acggatgttc
     8581 cggaacttgt tttcagtccc gcatgcccct gtatctcatg gggcggtaac cgctgttttc
     8641 cagcatggcg cggctgaaca catgctccga acatttccag gccggcaggg ggccggcttc
     8701 actgcccagg aacctgttct tttccgttcc ctcccactga atgaacagga gcacatggcg
     8761 ccccggcgca atcaggatgt ctcccgtccg gaggtcttcc caggagggaa gccgcgtgca
     8821 gagggcgggg agttcccgtg tggaaaaggg cctgtccagc cgccagcagc gggagacgaa
     8881 gccggagcag tccactccgg cggcaaagcg gctgacggcg gcgtctcccg ctgcctgctt
     8941 ttccggggtg cccatatctc cggccgcggc gggaagccct ccgttggcgg catccgcctt
     9001 caggcgttcg gagaactgcc gcggggtgtc gaagccgccc catttgtacg gcatgccgat
     9061 ggaacgcatg cccggcttcc accatgctcc ggcatcatat ccggctgcgg aagcgtccgg
     9121 cgtgtctgtc cggatgccgt ccccgtccgt cccgtggcgg acattccgga gagagccccg
     9181 ccaaatcatg gaggtatagg cccggctggc tgccagggct tcacggcggg tgacggaaga
     9241 agcgggaggc gggatttccg gtattcccgg ggcacaggct cccagacagg cggagaagac
     9301 gagcagcgtc agggcattgg tcatcccggc agtatggctt ccggcctgcg tggcggcaag
     9361 gatgattgct ggagaacgtt ttttattcag gggacggacg gtctggaagc ttgactacgg
     9421 ggaggaatat gtaaaaaagg tctcatcaag gcggaaggaa acggaaaagg tttccgttct
     9481 ccttcatccg ctttccatct ctttattttt atggacgaag aacaacaaat catccaggaa
     9541 ccggttcagg agcccgcccc tgagaaacaa tcggataaaa cgcaggctat cctgacggcg
     9601 gtttctccgc tggtgtctgt ctttttcgga ggctccgccg gcgtgaacat tattccgccc
     9661 ctgatctgct ggctggtctg gaaggatgcc aaccctctgg tggacaaagt aggaaagaat
     9721 attctgaatg cccagatttc ctgggcgatt tatacggttg cggcctgtct ttcatgtttg
     9781 atcctgatcg ggttcgtgct cgcccccgtg gtagtcatcg cttggatcgt tttctccatt
     9841 attgctgccg tcaaggcggc gaacgggaat tacgattatg tggctccctt cacgattaaa
     9901 ttcctgaaat aacgtttttc cgtttttcag cagatcaaca gatgaaaaaa ccgccggtac
     9961 ggaatgccgg cggttttttg atgagggctt tccatttgcc ggaggggttc agatgggcat
    10021 gtccttttca ctgccgccgt acatgaacgt atggagcagg cgggtaacct gattctgttc
    10081 cgcgtccagc tgggcggagg ggcgcagcgc ggcgtacgcc agtccgtcat gccccgtgaa
    10141 ctccctcata tggaacccct ggaagctggt catgggttca ctgtcatgaa agagcttgcc
    10201 cctacggagc agttcgtcaa agacatgttc cgcttcccgc gtgcgcgtca gagcgttttc
    10261 cgggtggggg atgcggatta ccagcgctcc gccgtccccg cggaagttga tgcccgctcc
    10321 ggccaggacg tccgcggaag gctggctggt gacgctgccc agcagcagca cgccgtcgtc
    10381 tatcagatgg atgcggctgg cgcgggcttc ctcctttacg tgcagaacgc ctccgtaaat
    10441 gcgggtggat gcattgccgg agagttttct gatacgcatc aggccgtgcc tgatcacgag
    10501 ttccgagccc tccgccggca gatccacttc cgaggcgaag agccagccgt ccgtcatgga
    10561 gattctgcct tcatgctccc tggtgccctg aatgcgcccc tggcccatca gaaggcggcc
    10621 gccgttgatt tccaggctgc cgttggcttt gtcccccagg aaaacggtca tatggtccgg
    10681 caggttgccc aggagcgtca tggcgcctcc gttccggatg acgaggtggg cggtgcggtg
    10741 cccgtggtcc ggctggttgg ggccgcccac gtaaacagga gcgttgatga tgtaaagatt
    10801 tccgccggaa tcaacggtaa gggaatcctg aatgtccgtc tggccctgca ctacatcatt
    10861 gctgatggtg accgggcaca tgtaggagac acggttcatg gatgaggctg aaagcggcat
    10921 catccctgtt agaatgaatg ggttgatatt gctcatggct ctggttacat agttggtttg
    10981 acgtttgagg cgcgatggat gcgctccaat ggtattagac catccgtccg gggaaagtgt
    11041 tcgcgtaaat ttcccttatt tttcccggaa ggggcactgt tcattcccac tccacacggg
    11101 tcagctccag ttcccgcccg cgcaggatgg ccagggccgg ttctccgcag ccggggcaga
    11161 tgtagccgtg ttccgtaaag gaaaaatccg cccggcatgc gtcacagcgg cacacggcct
    11221 ccacccaggc gacgtccagg gctgcatcct gcgccgcata cgagtctttc agggccagga
    11281 aggcggattg aagggcttcc ggcaccacgc cgctcaggct gccgatggtc atgtgcacgc
    11341 gcctcagcct gttccctccg cgtttttcca taagccgccg ggccatgtcc agcgcgcctt
    11401 ccatgatgcc tacttcatgc atgggcgttt ttccgggcct gatgttccgc cagggcgcgg
    11461 gcgatgaggg cctccgccgt gtccagcgcc ttcggcaagg cttcctgcac ctccgcggac
    11521 atggggcggc agaaatccgt atgcagggcc tccactccca ccagcgtgat ggcgcgcggc
    11581 agggtgccgc acagctgggc cgcccccagc acttccttca tgcctatctg gtggggagac
    11641 ataacgcggg aaaagtggcg cggcagttct tcccggctgc gcgtgacgac ggttcccggc
    11701 agggcccccg tcctgacggc atccagcagg atgagcgtgt ccgcatcttc cagcagggga
    11761 agcagggtca tgccctgcgt tcccccttcc tccacgtgga ccagcgggcc gtagtcccgt
    11821 tcctggaggc ggtgggccag ctccacgcct gcgccgtcgt cccccataag cgggtttccg
    11881 atgcccagca cgagcacggg ataggcattt cccttcacgg ggcagccccg cagttcttcc
    11941 gtgtatggct ccaatttcat gtcaagggga gggcagagag gttattcatc ccggaatttg
    12001 acgcccttgc gcagccatcg tccgccgctg aacatggagg agatggtgcc gtcccggtcc
    12061 acgttcccgg aaaggatgga aaggtacacg tggatcatgg cgaagatgat gaagcaccac
    12121 atgccgatca aatgcaccag gcgcacgtac ggaatgccta tcaggtcgtt cagcggcatg
    12181 aaccaacggt agaaccagtg gcgcggctca tacagggcat acagggccag cccggtcacc
    12241 accatggtgg tgaacaggac gtacacgccc gtgtatgtcc actgctgcag gggattgtgg
    12301 ccgatgtagc gggggccgtc ataactgcgg gcgaacaggt agtccaccgc tgtggcgtac
    12361 aaatccttcc actcctgtct ggaaacgggg aacagggccc ggaaactctg gtatttgttg
    12421 gaggccatga agaagcagga aaaacgcagc atcatcacca ccaccagaat ccaccccagc
    12481 gcgtaatgca cgaagcggag cgttccgaat tggaaagccc cgtgcagatc ccccattttc
    12541 aggaaccacc cttccgcgat gcagatgcct gtggcggaca ggccgatgat gcagagcacc
    12601 cacgtccagt gcagcacgcg cagaggccag tcccagacgt ggaaatagcg gaaattggcc
    12661 ttggccatgg agaggcggcg cacgtccctc atggcgtgca tgggcacgac gcccaacagg
    12721 cgccacggcg cgccggaccg gtggacggcc acggccacgg gttcaaagcc ggagggggaa
    12781 tgctcttcaa aggccttgtt catgcgctcc ttttccaggg agctgacttc gcacagtttt
    12841 tccagcgcct tcagattgcc gcgggctacc atgaaagggg aggcgtccgc cttgtctgcg
    12901 gctttcagaa cagccgtgct gtacggcctt tcccgggagg gagcctccca ggagttttcc
    12961 tgctgggcgt aagggagggt tatgccgcgt tcccgcgcgg catggagcag ggccaggtct
    13021 cccgcgtccc tgctgccgtt gggggaggcg cacacggcca gagccaggat ttcttccgga
    13081 gcgtacaccg gactggccgg aatggcatcc tccaccgcca gggtgaggtc gttatcgata
    13141 agagtcgttt tcacgggtcc tgtctggtca gaatggcctc cccttcttcg tcaaacaaat
    13201 ggacggcgca ggattggcac gggtcatagc tgtgaatggt gcgcaggggc tccaccgggc
    13261 gggaggggtc caccagcggg tgtttcccgg tatgggccag ggaatattcg tatggcccca
    13321 tctgccccgc cgcgtcccgt ccggaactgt tccatgtgct ggggactacg gcctggtaat
    13381 tcgccgtctg gccgttttca atccgcaccc agtggctcag gctgccgcgg ggcgcttcca
    13441 cccagcccac gcccttgcag gaggagggcc aggtttccgg ttcccatttt tccgggttga
    13501 aggtggaggt ttccccgttc ctgatgcggt ccgtcatttc ctggagctgg tccaccatca
    13561 tgtccacgtt gatcagggcg tccagcgcac ggccgtacac gcggcccagc gtggagttca
    13621 tgaaggcgtt gtcgcgcggc aggcccagct tgtcgcaggc ggcatccacc cgttccacga
    13681 tggcgggaac cccctgggca tagccaatca tcatgcgggc gttcggaccc acggccatcg
    13741 cgtggccgtc atagcgcggg gctttcaccc aggtgtactg cgggtggtcg gaaagccact
    13801 tgtagggcgg cctggggccg ttgtagtccg cattcgtctc cccgtcatac ggagacaggc
    13861 ccgcgtccct gccttccgta tacctgtacc aggagctggt gacgaattcc ctgatcttgt
    13921 cctggtcaaa gggaagcacg ttcctgtaat ccccattcag aagcacgccg ggcttgatca
    13981 tgcctttgcc ggcgggggcg cccgtgttga tttcagacgg agccccggtg cagaaaaagt
    14041 tggggttgga cgcgccaatg tgggcgtagt ccttataaaa tccggcgatg gctacaatgt
    14101 ccggataata tactttgtga atgaaatcgt ggcaggtttg caccagctgc ttcaggtggc
    14161 tcagggaaaa ctggttgatg gcctggtcat tgttcatgtt gatggcgcag gccatgccgc
    14221 cgaccaggaa gttcgggtgc gggttcttgc cgcccaggat ggtgtggatt ttgatcatgt
    14281 cccgctgcca gaccagggcc tggagataat gcgccacgcc cagcagattc acctccggcg
    14341 gcagcttgta ggccggatgg ccccagtagc cgttcgtaaa gatggaatac tgcccgtagg
    14401 cgatggagtc cttcagcttc ttctgcactc cggcgaaata cgcggcggag gacaggggcc
    14461 agtcggaaag ggacttggcg atggcggcgg tttccgcggg gtccgccttc agggcggaca
    14521 tcacgtccac ccagtccatg gcctggagct ggtaaaaatg gatgacgtgg tcctggatgc
    14581 ccagggctcc gctgaccagg tctcgcatga ggcgggcctg gacgggcacc gggtactgga
    14641 gcgcgtcttc caccgcagcc agggccgcga tggcgtgcgt acccgtgcag acgccgcaga
    14701 tgcgttccac aaaagcccaa acgtcccggg ggtcccgctt gcaggcgatc acctcaatgc
    14761 cgcggtactg cgtggtggac gtccaggcat ttttgatgac gccgtctccg gcctccattt
    14821 ccacccgcag gtggccttcg atgcgcgtta ccgggtcaat gaccacccgg ctggattccg
    14881 gaacgtccgc tgatgtgtaa ttgctcatgg taatctggtg gtgagggctt ctggctattc
    14941 ttcaatttcc ttgtcgggcg agccgggagc gtccccgaag gcgggcaggg agacgtttgc
    15001 ttcctccttc atgtgctgct tcctgtaatg aatggcggag cccacggcat gggcggcgat
    15061 tgccgctccg gcgccggcta cgcccacagc cccgatgatg ttggcggagg cttccacgcc
    15121 gaagccgttc acgttcggca gcgtcttgta aaacggagtc atgcggtcaa agaagtacag
    15181 ctccgtacag ccgatgcacg ggtggccgga ctggatgggg aagctgacgc caccgttcca
    15241 tttgacgatg gggcagggag agaacgtatc cgggcccttg cagcccacct tgtacaggca
    15301 gaagcattcc cgcgcgttct cgtcgtcaaa cacgttgacg aactgtccgg cgtcgaaatg
    15361 ggcgcggcgc gggcaattat tgtggatgcg ggtgccgtat gccatcagcg ggcgcgactc
    15421 caggtcgcat tccggggcgc ggtcaaatgt aaggatgtac atgatgacgg ccgtaatcac
    15481 gtccccgatg ggcgggcagc cgggcacgtt gatgaccgtg cggtccgtca ggatgtcccg
    15541 gatgcccacg gccccggtgg ggttgggacg ggcggcctgg atggagccga agtacgcgca
    15601 cgaacccacg gccaggatat gggaggcgtt ttccgcgcaa tggcgcagca tgttctccac
    15661 ggtatctccg gccacggtgc agtacacccc gccgtcattg accggaacgg aaccgttgat
    15721 gacgaggatg tggggctccc tgatcgcgtc ttccagagcc tgattggcgg cctctcctga
    15781 aggggccatc agcagttcca cgtaaggcat ggagacggcg gaaagcacca tgtccccgat
    15841 ttcttcgtta tagctgcgga tagtgctttc caggcatccg gtgcattcct gaagctggag
    15901 ccagatgact acgggacgtt tcaatgcggc catcttgtcc gcaatctcct gggcgttgga
    15961 aatgccgccc gttacagtgg caacgcccat cagggagatc attttcaggc accacgtgtt
    16021 gaattcgcgc cggctgacgc cgcggcgctc caggtgttcc cccagagttt ccccgggacg
    16081 ccatgnnnnn nnnnngcctg ctccgcctcc tgcatggaag gttgctgaat gcgcttcatg
    16141 tgcgtggtcc aaataagaac atttccattt agacaggatt tccgggaaat gcgatcaata
    16201 ttttccagtt ttccggaagg gcttttacca ggaccagacg ttctggatct ggaaaaccac
    16261 cgccgtctgg cccctgcggc cgttgacggg attcttgaca agctgaatat caggctggag
    16321 cgtcatggtg ggcgtcacct gggccacgta cgtcaattca atgccgtatt cattcctgtt
    16381 cacgtacggg ccgttctttt tagccccctg ctgccagagg aaaccgaggc ccaggtatcc
    16441 gtcattggat gcgccctttc cccggaaggg ggaaagaagc accagccccg tggtggccgc
    16501 gcagcgtacg ccggtcagcg tggctgcgtc atcgcttgcc cagccgccgc ggaagaacca
    16561 ccccaggtgg ctgctgcggc ccagctgctg ctggaagttt acagctacgc cgcccccgtt
    16621 ttcaccgttg ctggtggtga aaaagggctg gaggcggtac acgccggggc ccatgctgca
    16681 aaaatcttcc gccacgtagc cgaattcaag cacgttcgtc caattgtcgg aattcaggtt
    16741 ccggaaagga ttgtggtttt gagtcatgtt attggcggag gtggctccca tcacgtaaaa
    16801 ggaggtggtt ggctgccacg cgaaatggaa tcccagggaa ttatccgtca gggggagtac
    16861 gggattattg ccgaaaacgc tgttggtgag gttattgtta tgggaattgg cgtaagcgtt
    16921 gtggtccatg tagttggtgg tgtccagctg gcccgccatc agcagaagct ttcctcccgc
    16981 tcccacccat gccaggctca ggttgctcag gtgggggtcc gggccttgga aggagctggt
    17041 ggggttctgg agggagccca gagtggtttt ggggccttta ctgccttcat tgaagtcaaa
    17101 gccgttgccg aagtcgaact cggaggccag gtacaggccg ctgcccgtgg aatgattgcg
    17161 gaagatggtg atattggtca gcagggaacc attgtaggag gggaaatccg tttttccgtt
    17221 caggccgccg cccgtcagcc ccgtgtagtt gaatccgaac tgggcggaat aagtaatgcc
    17281 gtatctggag agagcggtct tgagcttctg cgctccggtg gaaatcatgt cttcgcgtat
    17341 ccaggcgttc ggttccgcgc aggtattgcc gaaaacgtag gaactgtcca ggtacggcat
    17401 cattttaaga ggcctggccg caaaccattc caggccggga gcggaagttc cgccttcatg
    17461 ctgaagcagc acgctgggcc tggtgggaac gccgggcgtc cttttggtga tttgttcttc
    17521 cgggcgcatt cctttcatca ggctggggct tatttccctg ccttccagag ttttcacgcg
    17581 cagaccgcgt tccgtgttca ggtaagcagg ggaaaccgcc gcagcgagag ccgcctgttg
    17641 ggcaagggcg ggactgcagg aggctgcgac ggcaacagcg cacatggagg gaagaatggc
    17701 atgtctcata cagacagtca gacaaggctt ttcccggaag tatcataact ccgtttaaat
    17761 gctgacacga atatcctttt ttcaccgtct ttccggaaaa aggaaaggcg ggctgtcctt
    17821 caggggcctt ttcctaagac aggcatgatg gagcaagcgg aagggcagct cccatcgccg
    17881 tattccttcc gcaaaagaag tggaaccagt tggaacatgg tagcttaggg agattattgt
    17941 tttttgcagg gggctgtctg aatgtacgtc gggtgaggga gccaaaagga gtgtaaaacg
    18001 ggaaaaaagc atcagatttt ctctgtaatt tttattgcga atttagaatc cgggaacaat
    18061 aacaaatatt gaaaatcaac gtctaattca tcttaatcag ttgtttttta atatcatata
    18121 taatttctgg tcttttttta tttcatctta tccgaataca ggtaaatatg ttatgagaag
    18181 gagaaaatat ttaagtgcgc agcggattct aaatccccta tatgtaccgc actgggagag
    18241 cgcgtcgctc ttttcttttc aacctagaac agccaaatga agaaaacatt atttttgatt
    18301 tcttgtgtag tagcttcttc cctca
//
LOCUS       DAWEMX010000042        18275 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1264_length_18275_cov_12.983699, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000042 DAWEMX010000000
VERSION     DAWEMX010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18275)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_18275_cov_12.983699"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3883..3892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4723..4732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3683 a         4372 c         6062 g         4138 t
ORIGIN      
        1 atgccctgtt aacgctgaat gcaatcaagg aacgcggcct ggaatgcctg ggcatcgtgt
       61 tcaataatgt gaaagatgaa tgggatactg cctgcgtgac gaacaggagc atggtggagg
      121 agttttccga tgcgcccatt ctcggcgaat tgattcacgg gcaggattac atggacatgg
      181 acgtcctgct ggaacgtttg cactgactgg aggagtctgt ctggcgaatg aacgggacga
      241 taccgaagac gcccgttcgt tattgccgga tttgcggagg gaaacagcga tatggaggaa
      301 taagcatgtc ggtgttggag aggatgggaa aagcatttgg tttcccagtt gagaaagccc
      361 tgttttgaca ggagcggaga tggaatgccg tagttaagga atgcttttgt tcagagtgtt
      421 cagcattccc gtacggtttt ttattgcctt gctgatttat ttttcatggg ggccgctggc
      481 cggaggggtg gttaccgcgc ctgtgccgga gttttcttcc cccgcgctta ttccatatcc
      541 ttccaaagtc gtcaggggcg cgggcgaggc aggtttcagg agcgttcatg tgaaggttgg
      601 tccggatgtt cctggcaggg atgatttgat gagggagatc aaggacatat tcaggacttc
      661 cggcattcag ggttccctga atagtgggag ggctgctgaa ggtgccatga catgggaatt
      721 gtttccggat gccaggatga aaggagaggg gtatgacctg tccgtcggat tgggaaaagc
      781 aaccgtcagg gcaggaagtt ttggcggttt ttttaacgcc cttcaaacgc tgcggcaact
      841 ggttttcaat agagaagggg aatttgccat gcctgttgtg cgtattagtg acaaacccgc
      901 tttcgtcctg cggggaatta tgctggatgt ggggcgttat tacatgtctc ctgccttgat
      961 caaggaggtg atgcgccgtc tttcccgcta taagattaat acgctgcatc ttcatttgac
     1021 ggatgatccc gcatggagga tggaagtgaa gaagtatccg gccctgacgg atggggcttt
     1081 tcattggaag tcccggctcc cggggcggtt ttatacacag gctcaattga aggatctgac
     1141 ggattattgc gcccggctga atatccaggt gattccggag attgacatgc cgggacacag
     1201 ccagcctttt gccagggcca tgaagaccgg catgcagacg gagaagggtg tttccatatt
     1261 gaaggatgtc gtggacgagg ccgtttccct gtttcccggg aggtttttcc acatgggatc
     1321 cgacgaggcg cacatttcca tgaaggattt tatcccacgt atggcggagc atatccgcgg
     1381 gaagggcaag gaggtggtcg tgtggtctcc gggcggcccg cacgataagg attccgtatt
     1441 gatgtgctgg ggagagaacg aggccggcgc caggatggat aagaatatga agcgcatcga
     1501 cagcaatggt ttttacattg actgggcgga ttcccagtcc ggcgtgtacc aggtgttttt
     1561 ccagcagccc tgcgaggtgc ctcaagggga tgataaggcg ttgggggcca tcatgcccgt
     1621 gtggtgcgac ggaaacctga gcagtgagag gcgggtgctg gaacagtatc cgttttatcc
     1681 ttgtgcattg acgtttgcag agcgtgtctg gcgcggaagc gctaccaaga ggagggatta
     1741 tatggcccag cttcccccca ggggaacgga cggctggaag gaattccggg agtttgaaca
     1801 gcgtttggct ttccatcgtg atcatttttt ccaaggcgtt ccttttgcct atgtgaaaca
     1861 ggcggatgtt gcctggagcc tggtggggcc attcgaccac cgggggaaga atgatacctc
     1921 ttttgaaccg gaaagaagaa tagccccttc ctacagggac ggagacagga tactggcctg
     1981 gaagaagact cctgtttacg gcgccgccgt acatgtcagg catctttttg cgatgttcaa
     2041 catgcaccgg aaccagtacc ggacggacca ctggcccacg ctgatgtcaa gggaggttgg
     2101 aaaggaggat ggaacctgct atgctctgac tttcatccgc agccccaggg atcaggaggt
     2161 gtggctgatg tttggcctga acggcatgtg ggggcattca ggcgggtacc gcagcgcacg
     2221 cgcccctgaa cagggaagct gggatttttc cggcggggac gtatggttga acggaaggcg
     2281 cgtgaaccct cccaggtggc ctttcaagag cctgccctgg acggggtggg gaaaggggcg
     2341 cattgaagaa gctcctctga cctgggaagg gtattttttc cgccctcctg taaagatcaa
     2401 gctccgcaag gggttgaacc gtgtattgat ccgcagcgtg ttcgggcact ggaagggaga
     2461 cgacggccag agaagctggt ttttctgctg cattcccgtc ctgtgggacg gcattcatta
     2521 ccgggaggtt cccggcctgg aatatgaccc ccgtccggat gcccgctgag gggaaccgct
     2581 attattcttt ttcaaagacg gagaggatgg cttgatggac atcctcagca gcatggattt
     2641 ccggcaggaa ggccgggccg atgaatacgg gctgctggtt tgcaggcacc cggtctctgc
     2701 catgggagcc tttcacctgg tcgccattga gggggataac tttcatcagg gcgcggaacc
     2761 ccagcttttt tttcagcaga aaggcggcgg catggaacat ggggaaggta aggcccggat
     2821 caaagaacat ttccgcgggg tcatagccgg gtttacggtg gatgtccacg cagcgggcga
     2881 agtccggcgc cttggtgtca tccagccagt aatagtaggt gaaccatgca tccggggctg
     2941 cgacggcggt gaattccggc aggcgttcca gagccgcgga agaaaggccg gagaagtccg
     3001 tttccctgat ttcttccact ccgggtgtgg cggagagcag tgctttcact tcttctttta
     3061 cggaaggatc attgatgtag atatgggcag tctggtggtc cgccacggca aaggcgcggg
     3121 aggcgccgca gtccagcatt tccgtaccca tttccggttt gacggtgatc cagccccgtt
     3181 cccggaagag gcggttgagg gcaatgctgc gggatacgtc ggaaataccg tattcactaa
     3241 ggacgacggg ggtgacgcct tcccgttcca gaaagtcgat caagtcgcag agaagaccgt
     3301 ccatagcctc tgccgcgtgg gcagcctggg tcgaggatgg tccgaatttc tgaaggtcat
     3361 agtccagata gggcagatag atgaggctga ggtcagggcg atgtttccgt tccacccacc
     3421 gggcggagtc tgctatccat tgggaggact gaatccctgc catggggccc cagaaggtgg
     3481 ggaaggggaa ttctcccaga tcctttttaa tggtttcccg gagttccatg ggttgggtgt
     3541 aaatgtcgaa gatcttgcgg ccgtctgccg ggtacatggg gcgcggcgtg atggtccagt
     3601 ccgccgtgga atacatgttg taccaccaga aaagtttggc gcaggtgaag ccggaaccgt
     3661 accgttccct cagtttctcc cagaggcgcg ggccctgcac cagcttgttg gactgcttcc
     3721 agaattggat ttcacacata ttccggttgt accatccgtt gccgggaatg gcgtgctccc
     3781 gcggggaaag ccctgtcacg taggtgctct gggcagagca ggtgagggcc ggaaaggccg
     3841 gggggaagga ggaaacgctc cgcccctccg cccaggcgga gannnnnnnn nngtttcatg
     3901 ggagggatat tagcggtttg gaacgggaat tccatctttc catttgaaaa agatgacggt
     3961 atccgtcatg attccccgca tggaacgtca ggataaacga cttgtggacc agttgcgtcc
     4021 gatcagcttt gaaacgggta ttgccccgaa tgccacggct tccgtgctgg tgactttcgg
     4081 acgcaccaag gtgatttgcg ccgttacgat tgaggaggat gtacctcgct ggatgaaggt
     4141 tcagcgtgtg gagggaggat ggctgacggc ggagtattcc atgcttccct attccacgct
     4201 tgaccgtaaa cgccgggata ttacggcggg gaaactggac ggacgttcca gcgagatcca
     4261 gcgtctgatc ggccgttccc tgagggcggc cgtggatctg ggcaagatcg gccagcgcac
     4321 gatttgggtg gattgcgacg ttttacaggc tgatggcggt acccgcaccg cttccattac
     4381 cggggcttcc gtagctctgg ccattgccgt gaacaagctt gtcgcggaag gaaagctggc
     4441 ggagtctccc atgaagaggc tggtttccgc cgtatccgtg ggaatactgg agggagaggc
     4501 tctgctggat ctctgctatg tggaggataa ggatgcggaa gtggatatga atctggtgat
     4561 gaccgaccag ggagagtttg tggaggtgca ggggtccggt gaagaggccg tgttcacggc
     4621 ggatcagatg tgccggatgc tggaattagg ccgcaagggg ctggaagaga tcgcggaact
     4681 ccagcggcag gtgattgccg aggaggacag gccgtccgcc ggnnnnnnnn nnagagttca
     4741 ctccttgcag gaccaagaat attatgagca taggcttgac ctgactggga aatcgcgtta
     4801 atattagaaa cgaacataga taaccacgta cggttttctt aaactgtaaa cattccatca
     4861 tatccaacaa tgaaagtatc ttttgcaaca caccagttac gcagggggtt taccctgatt
     4921 gaactcttgg ttgtcattgc cattatcgcc ctgctggctt ccgtggccta tggtcctatc
     4981 ctgaaccaga tcaacaaagg cgaccagatg caggccctga ccaacatgaa gaacgtgggt
     5041 gtggcgatga acgagttcaa gtccaacagc aaactgggca atttccccga tgacatcact
     5101 gccgaccgcg ttgttgccca gcataattat atgagcggcc tgggcgcact tcagggcgat
     5161 acttccaatg actatttccg tcagcttctg ggcaatgagt ctgtttccga aagcaacttc
     5221 tacgccaagg ttcagactcc ttccggcggt tccaccgtta ctcccaacgg tgaaatttac
     5281 gatggtcagg ccctgacccc cggtgaagtg ggtatttcct acgtcatgcg caagggtgac
     5341 aataacaaga aagtgggtat tggaagttcc gtgggggaat atcccctgat ggtcacttcc
     5401 gtgcttcctg gtgaagacgg cagcaccgtt gtggctggca atgccgtgcg ttttgacccg
     5461 gaaagcttcc gcggcaaggt tctgattttc acgactgccc agagtgccaa gaccctggaa
     5521 ctggacgaca acgacaacct tcaggatacc ttcattccca agagaagggg caaggacatc
     5581 agcgaccagt tcctgatcct cacgcctgat ttcagcggcc aggagtaaat tcgccgccgg
     5641 catgatttgc acagggcgca tccaaaagga tgcgcccttt ttcatacctg atttttgaaa
     5701 aggaaagctt ctgttttgcg gaaatccctc gctgagttga tcgggtcttc ccgttgtttg
     5761 cttaaaatag attttacgat gtaaggaaaa gcgccaggtt ttccgacggc agggagaaga
     5821 ttgttcgtac aaacaggaaa tggaacagaa gccttccgga gatgatatcg tggctgattt
     5881 cagtcggcag gcgtttttcc cggggttctg ccgttggcat ggggttgcag ggctttttcc
     5941 gatttcaata tgttccgcat accggcggtg gctgaaaaag gcagccatgc aggagccgtc
     6001 cctttggact tttggccgtt gcggcgatat acgggacatt cccagcctat tgaagcgctg
     6061 cagggggagt tggacggaat tctggcctgt cctttcccgt tttccttgat gccggtttgt
     6121 ctgccggagc ctgttttgca aatggggccg tgccgcaaaa aaggcattcc ggacgtaaaa
     6181 aaccggaatg ccctggtgag aaaagtcagg gaagttttta tttgcctgcg ggagtgccga
     6241 aaacgtttat ttccgcagcg gaagcgctgt tgccgtccag agaactggtg ccgatgaagc
     6301 ggaagtaacg cacgggttcg ttcatgtttt tgagattaac ggattgctca atggggttgg
     6361 cgcgcaggtt ggagaattcc ccttccgcca ctttcgtcca gtttttccca tccgcgctta
     6421 cttcaaattg gtatttatcc gtcatgccgg aggtttttcc atcctgtctg ggcaggtagg
     6481 agaagctgga aacctggtac ggttttccca tatccaccac aaaggattga ggcgcttccg
     6541 ccttggcaag ccatgaggtt tccggattgt cgtctatggc cgccgccgca tttccggatg
     6601 tggcggtttt cactttccat ccggttttgc tgatgccgaa tttggcctgg ctgacagggc
     6661 ccaatttacc attggagaat tggcagcgag ccttgacaat gccgctttcc gtaaatttag
     6721 caccctggct gtaaacggga gatcctgctt tgggttcgct gccgtccgtc gtgtacagga
     6781 ttttattttt gccgtccgca atgatgacga ggttgtctcc gcggcggaag atggagggcc
     6841 gcacggcgcc cgcaggctgg cggaagaggg agaattccga gatgcaggga gtggcctggc
     6901 tgcccgtgat gcgcaggcgc agtttctggg tagtgatggg gcggttcagg cggcgcatga
     6961 cctggttgcc gatggttttc ccttcattgt cgatacaaac ccatttgcca ttgacgaaag
     7021 cgtcaatatt gaaggagtcc acgcgttgtc ccagacgtgt ttgttcgcgc agccggatga
     7081 cgtcgaaagt ggcgggtttg ggcagcgtaa taacggcggt gggggtcagg ttgtcatctt
     7141 ccacggccca gtaggtttca atgttgccgt ccgtcatgtt ggagggcgag aattttttgt
     7201 cgttgccgcg cgtctggctg gctgtgacgg ttgctcccaa cgcgtagtcc ctggcatata
     7261 gcttgtcgcg cagttctttg aattccagca gggatttgac atccgcagga tccagtctgc
     7321 ccgttttgtc cgccgccacg ttcagaatga ggttggcccc gcggcctacg gaatcaaacc
     7381 atacctgcat aagttcttca ggggatttga cgcgggatgt ctgtccctgg tgccagaacc
     7441 agccggcatg gttgatggtg gtgtctcctt ccgcaggcac ccagcgttct ccgccgcgtt
     7501 tgccggtgcc tccgcccccg ccgttcagcc ctacagtgct ccaatgggga taattgacgt
     7561 ggcctttttc ggagcctccc cagcgggcgt ctccgtagtg gcccgctccc cagatgatgc
     7621 agctgggctg gattttgcgg atcatttcca cgattttttc aaagttgtag tatttttccg
     7681 cgtcaccgat attgcgcttt tccctggctc ctccgtaata gccgtctcct ccgttggctc
     7741 cgtcaaacca gatttcaaag acaggcccgt aattggtcag cagttcccgg atttgtttat
     7801 agtaaacgtc caggtagccg tccttgccgt aatccgcgtt gttgcggtcc caggggctga
     7861 ggtaggtgcc gaatttgatg ccgtgctttt tgcaggcaag ggcgaattcc cggactacat
     7921 ctccttttcc gtttttccaa gggctcttgg tgatgttgtg gtccgtggat ttggtgggcc
     7981 aggcgcagaa gccgtcatga tgtttggccg tataaatcat tcctttcatt ccgccttttt
     8041 taaaggtgga gacgatatcg gaggcattga aatccgtggg gttgaaaatt ttcgggtctt
     8101 catcgccgta accccactcc cgtcctgtga acgtattcag gccaaagtgg atgaagccgt
     8161 agaattccat gcgctgccag ttgatttggg cgggtgttgg aatcgcgccg tatggtttgg
     8221 gtggtgctgc ggtccagccg ggcagcatgg aggaagagag aaccgttccg aggaagagtg
     8281 tggttagttt gttcatgcgg aaaaaagtgt gttgaaaaat acgggagtcg ctcctccctt
     8341 ctttcatacg gaggctttgc tgtcaagttg aaaggaagtg ggaaacatgg gggagcgtgt
     8401 tagtgaattc taactccgac atgcggggaa ttccgcttga tacggagtag ggagccgttt
     8461 agcatacaaa ggactttcgt tcccatgtcc tatttccgtt ttttactgct ggcgctggtt
     8521 tttctgaccg gttccgcgtt gatcagcgag agcgctcccg tttacatggc ccggcgggac
     8581 agcaagtttg cgcttgttcc tctttgtgac ggttttgatt ttccggtagg gaagcctgac
     8641 ggcaacggct attaccggtc ccgcggtctg cgtctgaaaa gcccccgtca catgggtgag
     8701 gattggaacg gcaataaagg aggcaattcc gatttgggcg atcccgttta ttccgtaggc
     8761 cacggggtgg taacttatgc ggcggacgcc cggggcgcgt ggggaaaggt ggtgattgtg
     8821 cgccatgctt tccgcgaacc caagtccgga aaggttcttt gctgccagac gctttattcc
     8881 catttgaacg atattaacgt tgtgctgggg cagttggtgc tgcgcggcac ccaggtgggc
     8941 actatcggca cgaaccgcgg catgtatccg gcccatcttc atgtggagct gcattataat
     9001 ccggatgtga attgcggcca tcaggggatt cccaagacgg agcgcaatta cggccgcctg
     9061 acggatttta ttacccgctt ccgccgcctg cctctggaga aaaggatggt gcgcgttccc
     9121 atcggcgggt ttctgcctta caagggaacg gaaggactct gacttctggt ggatgacggt
     9181 gaagctgtgt attttcggcg gttcgtttga tcccgtgcat gaggggcacg tttgcatggc
     9241 cgagcatgct cggaaatatt gcggaatgga ccgcgtgctg tttatgccgt gttccctgtc
     9301 tccgctgaag gagcaggccc cctccgtgac ggatgaccag cgctgccgga tgattgagct
     9361 ggctgttcaa gggctggact gggccgtgct tgaccggacg gatttggaac ttccgcctcc
     9421 ttcgtggtcg tggagggtgg ctgagaggat ggcggagcgt tatcccggtg cagaattgtt
     9481 ctggctgatg ggcaaggatc agtgggattc tctggaacag tggggacgct gggaatattt
     9541 gtccagcctg gtgacgttca ttgtttaccg ccgcggcggg gttccatcac ccaggaaagg
     9601 ggtgcgcgcc cttttcatag aaggggatga acccgcttct tccacccgca tacggcatga
     9661 tttgcgttcg ggtgtatgtc cggttcccca tctgaaccgg aaagtggagg cgctgatcag
     9721 gagggaaggg ctgtacggta cggcacgcgt ctagaaggac ggctgttttc agtttatttt
     9781 ctttttgtct ccgaacgttt cttccgggta atggaccctc atcagcgtga gtccgtccgg
     9841 cggggcgcaa aacgggcttt tgtcatccgg gagcggagcg ttgatgaggc gagccagttc
     9901 ttccagcgtt atttttccac gtgcggcttc atgggcggct cccgtcatga ggcgcaccat
     9961 tttgtagagg aagcccgttc cggtaaaggt gatgaagaca tgttcccctg tctgtgccac
    10021 ttgtgtctgc gtgatggttc ggcggaagtg accctccgga atagggcgcg gttcattgcc
    10081 gcgcagggcg gcaaaagcag tgaagtcatg cgttccccgg aataggtgta ccgcctgttc
    10141 cagaatatcc acgctccatg ccagcgggcg gtgccaggcc agcccggcgt caaaagggtt
    10201 gaggatgggt gcgcgtgaaa tgcagtaacg gtaggttttt cccgtggcgc tgaaccgggc
    10261 atggaaaccg ggaggaacgt attccgcatg ggtaatccgg atggttcggg gaaggcgcgt
    10321 gttgagcgcg gcaggccatt tgtccgcagg aatgcggtgg gtatccggag cgtctgcatg
    10381 aaaaacctgg cccagagcat gaacgccggc gtctgtcctg ccggaaccgt ggatacggca
    10441 gggggttcca aagagtccgg aaaaggctcg ttccagcacg tcctggacgg tacggcctcc
    10501 cggctggctt tgccagccca gataagggcg tccgtcatat gcggcggtga aacgaatgcg
    10561 ggtcataggg gtgattgtgt tggaacggcc cggttttggc aagcggcaag tgccggacgg
    10621 tgacggtttg gaatggatgc cgtgcgttcc atccgctctt tagcttggaa gccgttaaag
    10681 atggctatac gaatacagga ttttggaatg ttgcatgtgg ctccggattt gtccggcgtg
    10741 attccgttga atgcttttga agcacaggcc gccagtgtgc gggatctggc ggcgggcgtg
    10801 atggatgtgt ccggagtggc gggaaccgcg ctgaaggaaa tgcaggaggt gagggatgcc
    10861 ggccaatggg cgcaggcccg tgacgggatg taccgttttg aacaggacgt gatgcgggag
    10921 ctggaagccg gaggggattc ggaacacctt caggagagat ggaagaaggc gctgtcagaa
    10981 cggcttcctt cctatctgcc tgcccgtatg tccggcccgg tgagggagcg cgtggccatg
    11041 gcgagagaga atctggagac tgccgggagc atttatttgc agaagatgtc ccatctgggg
    11101 cagttggagg aggcgcgccg gtattgggcc gggggggtgg agtctgccgt ggagcaggga
    11161 gaggccgctc tggccgaacg caggattaag gagggcagcg gaatatttgt aacgggagag
    11221 gaggcttccc tcatggcgga ggaagcgcat tcccgcgcag cgctgaaccg ggcggtggag
    11281 gaggtccgcc gtgatccggc ggccgctgta agcatcgttt cccggcagga gaagatgccg
    11341 gaaactccgg aggaaaagaa ggtggcggaa gaggctgcgg cagcgtatgg ggagttgaag
    11401 cggcgttatg cggatgctct ggcggccacc gtgtccggcg gcgggttgct gcgggatgac
    11461 ggattggcga atgctgaaaa gcatggcttg atcagttccg agcagctaag gcgttatacg
    11521 gcttcccgtg accggaggca gacggcggaa ttttccggta tcagcattcc ggaggacgat
    11581 atgctgttgt gccgcatgac ctcgctgatt gatgaggatt gcggagggga ggaagatgct
    11641 gacgccctga tagaagtggc cacttcaggt ttgcctgccg ttcaggtgga aaggcttctc
    11701 cggcggcgcg aggtgatgtt gggcataccc cgcgaattgc gccgcgctgc ttcccgtcgc
    11761 ctgtccgcca tgttccggaa cggcgcatgg gggccacctg aggatgcccg tgcggcagag
    11821 gaatggaagc gtgtccagac ggaattggca gaggcggtga agagggcccc ggagcagccg
    11881 gctgaggaga tggaaaaggt ttttgagaaa gagaaacgct tccaggatgc cggctgggtg
    11941 gggttttcag atatgaataa cggaaaagaa ggaaagtgat gatggaagat aatggaacca
    12001 gggaacagac aggggcgctc ccgctggaag ggagttttcc ggaagcggtt cccggaactg
    12061 tgggggaaag ccatattcgg aaagagaacg cagcattctc cgtaccttcc ggcacggaaa
    12121 aattctcgga acgggagtcg gaaagggaag aatattggaa tagcgtattg acgggggatg
    12181 tgatgaccat tccgcgggaa gtgaggctga aagcggaggc gatgtatccg gaagggacgg
    12241 cggaccggga aagaattgcc gcgtgcgtca tgcagtcctg ggtggcggat tctggggcga
    12301 ttccccgtga acgtatccgg agggactgga agagtatccg ggaacaggtg gcgcggcagt
    12361 atggagcgtc cggcaagtct gatgacgaat tgttcgtggc agtttccatg cacaatcagg
    12421 tacgcctttc ccggagggag gctttatttg aattatacca ggaagagtat gaacgtactc
    12481 tgtccggtgg cgggacggtt cctgacgaac ggggaaggga agccatttcc gcatggccgg
    12541 aagatttgca ggctgtggcg cgacaattga aaggcagggc cgtatccgat gctctggatg
    12601 acaggaaccg tctggcggat gccgccggga tcatccgcaa gggattggag gggctggtag
    12661 ccagtgagga gccggtgaag gaaggttttc ccgccataca gctgaaccgg aaagccgttg
    12721 ccggagttcc ggacgtcata cgcgccgtgg attgcctggc ttccctgggg gatggagacc
    12781 gtcagaaggt gttccgcatg atggctccct atttgcgttc ccgtcccgcc tttcgggatg
    12841 ccctgggggg ggcgcttaaa cgcggtgcgg cagaggttgc ggagaatgcg gcccagctgg
    12901 ccgtaaacgg cgcagcatgg ctcctgccgg agggacggga aaaggaggcg ctggacaatt
    12961 attcccggag ttttgaagaa ctgaggaatt ttgccaggat ggagtttgcc ccgctgcgtg
    13021 ggggaaagga ttccccctgg ttccgggaga tgctggtgga catggcccag cagggagcgt
    13081 ccacggcttt ggcgttttgc ggccctgccg ggcttggcat gttgatggcg ggggagacgg
    13141 ggcgccatat ggcggaggcc cggagcatga acccggacgg cgtgtttgat gcccagatgg
    13201 ggggagctgt cgtctccggc ggggtgaata ccttgctttc cttcggaatg acgaaactgg
    13261 gccagaagat gctggggcag ggaatgcggg cgttccgggc catgaggccc gcatcaggga
    13321 atcctggtgc gtttttttcc ggaatgggag ccgttgcggc ggatgccgtg aagatggagg
    13381 cggaaaataa attgacggag ttgacgccca tggtcgttca ggaaggcgtg ggagctctta
    13441 cgaataaggc gtccggcgtg gactgggagg aatggaaggc cagacaggct tccgtggatg
    13501 accagttgag ggaggccgtc atgatgatgc ctttcctgct gataggcgcg ggaaaggtcg
    13561 ctctgaacca tttccgccat gccggaatgc tgcttggaga cggttcccct ctgaaagtat
    13621 tcggcattcc ggatgaaacg gtttcccgga ttttgcagga taaggatgta aaccgtgccg
    13681 gggaattgtt gcagaagtcc ctgcgtaatt ctccgctgtg ggggtctttg tacatttcca
    13741 ggaaggcttt ggagtggtct cgcgccctgg atgaagccgg agagcctttt ttgaagacgg
    13801 aacaggatgt aagagatttt ttggatttgc cttctcctgt acgtacaaag ccttggaaga
    13861 tgcgggattt ccccgaatcg gaagcggtgt tgaaaaagct ttttcctcat ccggattata
    13921 ttacggccag aaccaagtgg ctgttgcgtg ccggtctgcc ccgcgtgggt gaagccgccg
    13981 cagcggacgg gcagcttatt tatgcggcgg aaccggctcc cggtgtggag atgaagcgaa
    14041 cgcagggccg gccttccgta gaggaggctc tttcttcctg gtatgagact ctgggccgta
    14101 tggaagagga agatttgggg ctggtccgga acaggaacgg acgggaaatc ccatggcgtt
    14161 taaggaatgc ggaggaatat gatattcatg cggatgcttt gcgccgagcg tttgtggcgg
    14221 acaggctgaa gaagcgctcc tatcggccgt acaggatgtt gctgctggct tatccggaag
    14281 aggcgggacg ccgtgcgggt ttttccggca gggactggga cgccgttacg gtggatatgg
    14341 atttgcggac acgagccaat gtttatgaag gagtaatgga acgggtgcac ggcgtgccgc
    14401 atgaagaggc ggccgggcat gtggcttttc gtttatggaa gtcgttttgc cgggatgaag
    14461 gcgcctgcag cagggcgcac cggtggctgg aggaagggag tgcgttgctg aatgctccgg
    14521 atttgttgtc tgaatctttt aatccggacg gccttccctc cgccctggcc aggatgagcg
    14581 gcatgatggc ttctcttggc ggagaggaac gccatggggt aagccctgag ctgcgggaga
    14641 tgaaccgcat ggtatgggga acccaggcgg atgtgaacag cctgtaccac gtacttccca
    14701 acatgaagga atttgacgtg catgtggggc gcggctatac gcccgtggag agttacgggc
    14761 ggttgctggc ccggtttctg gaggtggcgc cggagaacgt ggcccgttat gcctctgtgc
    14821 tggatgctcc gcggctgcat gccggtccgg aacaggtttc gccgcgtatt tatccgggga
    14881 cgcagaaagc ggtggagagc cttctcctgc tgaccccgcg cctgttccag tcctcacccg
    14941 gaggggaagg tgcggaaagg ctgtggagag taaagtatcc caacggaagg tggtcggact
    15001 ggcatccttc tcgggaggcg gcggaagcgg acctgatggc caatgtttcc atgatgtttt
    15061 ctgccgcctg ctcggctaag agggatttga tccgtagctg ggaatggcat gcccgcaacg
    15121 ggcggccgga atgggatacc aattatctga ggctgaccac cgggttggaa aacgtgcagg
    15181 gggggcgact cccgtgcctg tatgacagcc tgacggccat ggcggtggcg gatatgctga
    15241 aagctggtta cggcgtgcgc ggagcggtgg ggtccgggga tcgtctggag gtgacgggcc
    15301 gggggcgcct tgccgctgaa acgctgatga gccgtgatgc cctgttggag cgcatgaata
    15361 ttcacgaaaa gtatgtgggt tccgtacaga ggctgggcgt ggatgcggat aatttgcccg
    15421 tttcctcagc caaggggttg gtaatgcacc ggctgaggct tcacaatacg gccaatcccc
    15481 tggctttgat cgaagacaag gcggaagtcg tatgggaccg gctgatgcgc acggatcagc
    15541 tttccccgga aggcgcttgg gagatgttga aacatttgga acgtgtgccg cgccgcagga
    15601 agcttccagg ggaaacggaa ctgattgaag agttgtccca gctgtccaag gagtattttt
    15661 ttgcccatct ggatcatgac accgtaccgg aaacggtggc ttcctgggcg cgttacgggg
    15721 ccgcaatgcc tgacagggtt ccggaacctc ttgaggaatt ggtgcgccgg gccggattgc
    15781 tgaagaaaac gctgagggag gatgcggaca ctgccgagtt tatgggaatg ctccgagaga
    15841 cggttggtat gaatgaacgc atacgggcgg agcgcgcctg ggatccggcc ggaggggagg
    15901 tccgcctccc catggtggag cggtactctc acagcctgat gtccggacgg ctgttgttta
    15961 ccgttccgga tcacgtgaaa gagggactgg tgcgttccct gtccgcgctg gacggatacg
    16021 cgggcggttc cgtacgcagg atggagcggc tggcggaaag ccgcatgagg gaactgtccc
    16081 gggcgcttca ggagttcccc ctgctgaatt cctggcgtcc ggatccggaa catcccggcc
    16141 tgtacctgca tttggtgaaa aagccgttcc ggaccggatt gggacctctg cgcgcgaagg
    16201 atgtttccct ggaggctttg ggagaacatc ttccggaacc cattctggaa gcgcccctgt
    16261 atgtggaaga agatttttcc gtcaggcgtg gcgttcccgc ccctgccgtc tggcgtagcc
    16321 gtccggatgt ggtccgggct gtggagacgc tggatgcggt gaggcgggat tttgcaggcc
    16381 gaccggaagc tacggaatcc ggggttagtt ggaaagggaa gatttaccgg attgattccg
    16441 gagcggctcc cgggggggtg acgtctgcct ggaaccggga actcccgttg gatgaggcca
    16501 tttccctgat tggtgttctg gaccgcaggc aggaggaact gagaggcggc atgcttccct
    16561 ttcttcctga gccggaggaa gcgcgcgcga tgtatgccaa ctgtgtgatt taccgtgacc
    16621 cggacgaccc ggggcatacc gtgcgcctga tgccgggcat tccggaaagt cccgtacggc
    16681 aggcacgcgc gccctacgtg gtacatgcat ggaacggcgt ttatctggac agccggggag
    16741 cgcctgcggt gtccgaacgg gattcctata ttccgctgga gtgttttacg ggagccggag
    16801 aggaaccttc cccggaagat tcacgggcga gccgtacccg gttcctgggt cggattttgg
    16861 aaacgctagg ggatgcggat gctcaggaac gatggtggtg gaacagaaga caggctgtgg
    16921 gatgcttcat ggaggatgta atcaggatgt atgaagaact gggcgtgcgt gagagttatt
    16981 tgaaaggaaa acttgatctg cttgatcccg tgatggtgca ggcactccgg ttcgtttccc
    17041 acgtgctgaa cgatccactg gcataccgcc ttcccctgga caaggggacg gaggcccaga
    17101 aggggatgac cagggaagga gccctgttga aagaattaat agaggagtat ggaaaattct
    17161 aatcatttga cggaatcatg gagcctgccg tggcagtccg tcctgtccag ccagctttgg
    17221 aggctggaac acctgtactg gatagaaaat aaagccggtc agctccagcg tttttccctg
    17281 aacagggcgc agcggcggct gcatgagcgg ctgtggtaca ggaatgacat cctgaaggcg
    17341 cgccagttgg gcatttctac gtatgtggcc atgctgatgc tggatatgag cctgttccgg
    17401 tccaatttcc attgcggaat cattgataaa agcctgccgg acgcccagca gaaactggcc
    17461 aagctgcatt ttgcctggga ccatctggat tacgtgccgc ccaacccctc tgcgatggat
    17521 gtggccctgg cccggctggg agagagaatc aagagcctgt ccggcgtgga aaaaaagggg
    17581 gaatggcaac cccgtacgtt ggcgcgcagc cgcctggctt tctccaatgg gagcgatgtg
    17641 cgggtgggga ctaatctgcg agggggaacc atgcagtttc tccatgtttc cgagctggcg
    17701 tatgtttccg tgcatgctcc gtggagagcc agggaaatcc gtacgggggc aatcaataca
    17761 gttccccccg gaggttttat tctgaaggaa agcactcatg agggcggacg gtacggagtg
    17821 aattatgaac tgatgcgcca ggccatggaa aacatgggca aaagcgagct ttcccccttg
    17881 gatttcagat tctttttttt cagctggttt gaccaggatg agtacacttt gccggggcgc
    17941 ggcagatgga gcagggaact ggatgagtat ttcctttctc tggaacggga gaccggagtg
    18001 gttctggatg cggggcagaa gagatggtat gcccggatgg cccgggtgat gggcgcctcc
    18061 atgaagcagg agtatccggg aacgccgcag gaggcttttg ctacagggga agaaggcagt
    18121 atttacggca gccgtatcat ggccctgcgg gagcgggggc gggtaggggt ggagtttgag
    18181 gcagatccgg acgctcccac tttcacggca tgggatctgg ggctgagtga ccatacggcc
    18241 atttggcttg tgcagatcat gggagacagt ttcca
//
LOCUS       DAWEMX010000043        18102 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1284_length_18102_cov_11.656619, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000043 DAWEMX010000000
VERSION     DAWEMX010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18102)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18102)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18102
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1284_length_18102_cov_11.656619"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    17455..17464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3876 a         4666 c         5450 g         4100 t
ORIGIN      
        1 cgttctgttt gatcgtgtcc agcagttctt ccaggatgcg gcaatgccac tcgggattat
       61 cgcactccag gcggatcatg ttaaactgaa accattggct cttttgttct ttttcatctt
      121 cctgcatcag gcatgcggca tatgcctctg ccagggagtc ctgataactt tcccatccct
      181 tctgcgccgc cttcaatttt tccgcgatgg ccggaacatg ggagtaatgc ttcatgaggg
      241 ccgaatgtgc tttatgaagt cgtttttccc aatacgcgga cagctcctga cgtacgtaat
      301 tgctctggcg ccccgtgagt tctcctccgt caatctggtc gagcaatatt tccagttccg
      361 ggtcaggagt tttttcttca tccaacaaaa tagttaccgg aattccggtg aggtcttccg
      421 cttcctgttt ttgagcgcaa gtaagatagc cttgaagaat ccctttgtaa tagcaatagc
      481 ctcccttgtc tgtgaacgtg atggtggccg gcttcgggtt ttctcccttt gccttttcat
      541 aggatttctc ccctgataaa taatagcgtc tgaacaggga tgggttcttt tcgtatattt
      601 ccccggaaga ataatcctct ttccgcttat cacaatgaac gacaatgata tgagcgtttt
      661 tccccttggg ttcgtatgta acgattctat cttcactacc ctcccaggaa atgccgtcct
      721 ttaaattcag aaaaacatca tgatgcaaat ttttatattc ttcctttctt gcggaaattt
      781 ccagcgatgc cccctccaga gacggaggag attgaacttc tccggcaaat gaccggctcc
      841 agcacacggc ggcaatgatg aggaaagaaa aaatgggggg aataggtttc atgagtttat
      901 tcattagttg atgctggaaa aaatgctatt ccaatgagac aggattttat tggtgtccaa
      961 ccagggatgc agaataatgt aatttattga aatgccggct atggccagcg taaatgattt
     1021 atgagcggga aaaaatccgt tttgctcgta tttaatgcgg ccccatgcaa aacaggcgag
     1081 caccgatagc agcggaagag cataaggcca gcacatattt tcaaaaagat aataggaatt
     1141 cctgaaaagt aacatggaca gccatgagaa cagaatacct gcaatgagag caaattcgaa
     1201 ccttacggac tgataataac atttcgccat aaaaatgaga aaatatagac tgaaaatgga
     1261 tatcatggtg taaaagaact ctccttggta ggaggtacgc agaaatccca tggatccgcc
     1321 aatgaaaatg gatgacaaaa atatggctgt taaaggatta tggtgaattc cagtaattcg
     1381 tttaaaaaca gggctacaca cggagagcat aagaagggcg gaacctgata tgatgcaggc
     1441 catattgacg taagcggcat tttttactga cgggaataga tcgcctgtaa gtagaaaaat
     1501 aatatacgga tctgaaaatt ccgactttgc gtcaatagtt attccaggga taagcagggt
     1561 aatgataagg aaaaaggaaa tcagggaaga aacagggtgt ttttggatgg gatagatgat
     1621 gtcgtaaaat gaatttttat aagaaaagcg tcttttccat aagccgttta tccattttcc
     1681 tgcttctttt ttcccctcag gggagagcat gattcctttg ccctgcaacc cttcttccgt
     1741 atcgttccac gtacctttgt agatattgcc gtctttccat gtcatcattc cacggccttt
     1801 tctggctgtt ccgcaaaaag ttccctgatc cgtccgctgg tgctgagcat ggtaaaaggt
     1861 ggcatttccg gaaagcttgt cgttttccca tgtcccttgt acccaggaac catcgctaaa
     1921 ataatagatc cctttcccgt ggcgcaggcc gttcttgcat tctcctgcat agctttctcc
     1981 atgtggatag gtctgcgtgt tgtgggaatg tccgttgttt tcctcaggac aggtttccgg
     2041 cggtgtagag acgctcagca taaaatagag gaattattcc gttttttggt taaaagcaat
     2101 acatccggca tggaatgcct gcttaatgca tactatccag acaatagggc cgatgatggc
     2161 ggcccctatg gcgaggccga ctcccagccc tccaaggcga aatccccact tttccatgga
     2221 agattccatg tcgttacctc ggatagtgcc tctggtgccg tctttgaata caatgctttt
     2281 accttcgaac gcatccgcat atttatcttc cgccttccat tcttccctcg tttcttgaat
     2341 gacgctgccg aaagtgtcct cttctgggag gagtcccgaa ccctttccta tcccgatagt
     2401 aatggcgctg aagatccata acagagtcat gcttgcaaaa aaaccttccg tcacgtaggc
     2461 gtaaacggtc acgatcagac ctgttcccat caaaggggcg attagcagga ggcctatatt
     2521 ttcagcattc ggctttagtg tcttcctgca aaaggccaca aagcataaaa tcaaggcggc
     2581 cagcgagaca gccatgatga acacgatgat ttcttccgcc agcgtcaggg aataccctcc
     2641 ttctagggat ttcccgatga tctggacaat attatgaaag acgtccataa agttttccga
     2701 ctttttcatg gaataagctc tattcagcaa cagggttgat gtagagggga agatttggtt
     2761 gcgaaccgtt caagccggga actccggatt aaagatcgca gctttgaatg gtgttgagca
     2821 gttcttccag aatgaggcag cgcgtttccg tcatttcaat aatcatgagg ttcatatggg
     2881 cgttgaacta gccgttttcc ctgccagttt cattctcttg cctgagacac aggccaaagg
     2941 aattattttg gcaggccttc ttttgatggg agactccgtg cagcgtcatg cgactgcgga
     3001 tgcccaaaga ataagacctg catttgctga tagcggattc ggaatccgta taggaattcc
     3061 tgtgtcctct ttgtcatcaa actatctgga agggaattgc tgatatgacg gtgcggtgaa
     3121 acggcatcca tggaattgcc gaaccttttg gttaataaac gactattttt gcttgactac
     3181 ggattccgaa accgttgcaa aatagatgaa atgaaagagg aaatttcagc aacggatcat
     3241 gcggcgctgg ccattttgaa gaagacaggg ctggatgtgg tggaagccgc ccagctggcg
     3301 catgaattgc tgcaggccag caaggggcgc ggcaaccgat ggaagagggc aagggaatgc
     3361 atccggctgg gagtggaagc cctggttgcc agaggaaaaa ccgtggcgtt caggaaagcc
     3421 gtggatgagg ccatggaggc ccggcaagat cgcaggaaaa gaaccaggga tgatttccgt
     3481 tatatcagcc ggcggttgtt aaggttttgt ccggaaatgg cccggcggcc cgtgcacttt
     3541 atcacgcctc gggaatgccg cgcctgcctg gaaaaatcat ttgataccct caggcagagg
     3601 cataaggccc ggttggtgct cagcggtatt ttcggaacag ctgtgaaacg cggctggtgc
     3661 acggaaaacc cggtggcgta tgtggatctg ccgaggctga aggagcattc cattcccgtg
     3721 ctgtccctgg aagaaatgcg ccggttgttg gctgcggcgg aagagtatga cggaggggcg
     3781 tgcctggctg cggttgggct gatgctgtat gccggcatcc gcccggaaga agtgagaagg
     3841 ctggattggg cacagattaa tttaagggag ggaatggttt ccctgagggc gcgccacagc
     3901 aagacgggag gagcccgcat tgtgacgatc cagccggttt tgggaaatct gctggaaaga
     3961 acggttgcta tgggatttgt gtcgggaagc gtatgtccgc gcaactggcc ggcgaagtgg
     4021 agggagctga gacggttggc cgggtgggac gggaaggaaa agaagtggcc tgcggatatg
     4081 ttaaggcaca cgtttgccag ttattttgcc aggcatttca agaatttgca tgtattgcag
     4141 atggaaatgg ggcatgcttc ttccgatttg ctaaggacca ggtatttaaa tatggaaggc
     4201 attacggaaa tgacggctgc cgttttttgg gggaactgcc atgcggggcg tcacaccatt
     4261 aacaatggcc gcccctgaac agaagcggcc agctgttgac gggatgatac cgtccggcaa
     4321 ttagaagttg gtgcggaagg cgatgttgta gctccatccg gtaaagcctt tctgccggct
     4381 ggcgtccgtg ccgttggaat gggcgtattc gatgcccgcc atgatcttgg ccatgttcgg
     4441 gctgcattcg aaaatgtagt agttgacgcc caggtacagg gcctgcatgc agtcggaagt
     4501 tccggaataa gtggagttct gcgtatagcg tttttcccat tttacggcgt tgctgcccgc
     4561 cgccatctgg tagcggacca ctccttccca gtggggggtg aacttgtagg acggcatgac
     4621 ggtcacgccg aagacgtttt cagcccccgc tttcatgccg atgacgttga agccggccat
     4681 gacttcggtc atcagggaga aatccccctc ggaaccttcc caggtcaggg ctaccacgtc
     4741 cctggctccg gtaccctggt atttggattt aaagccgtaa ccggtcagtt tttcatacgc
     4801 cttgttttgg gtgtcgtccc cccacttggt gaaattgtgg gcgtaatcca gccacagacg
     4861 gcttttcttc aggaaaacgt cgttggacgt gtcatagctc agcgtgccgg tgatgaagca
     4921 gttgtcccgg gagttgaaag cgggttcaat gcggttgttg aacgtgccgg ttccggtgcc
     4981 tccgttgccg ttcagccaga cgcctgcggc ccagccgagc ttgtcctttt tgtcggaatt
     5041 gttcacctgg aatccgtaat tggtttccgg gcggagctgg ttgaccagca tggagcgttc
     5101 gatagtcaga atggcggagg aggacgttcg gtattccccg gtgatgcgcg gctttatttt
     5161 gccgatggca taggtgacgg ggcctccctt gtattccaga ttcagttcgt aaagggagta
     5221 ggtcaggtcg gaggtgtccc atgtgttggt tttcgcattg tagctttcca ggccgtccat
     5281 gccgccgatg ttccagacgt tgttgagctt gaaattgttc agaaacttca tgttgaaccc
     5341 ggcacggaag cggcgccatt catcgttgaa gcggcggccg ttgccttcct tttcacccat
     5401 gacgcggcct tcgccgttcg ggtcaatcca gtccaggccg tactgcatgc ggagggaaac
     5461 gttgaattcc tggatgtagg gattcttatg gtcccgttcc gagttgaacc acttgaaacg
     5521 gtcgccgagc catttgcagg cggaaaagtt gtcgcgggcc ggcagggagt ttccggtaga
     5581 gggcgcggca gcggcaaagg cggaggctcc gagagtcatg aggccgatga cggggagagt
     5641 ataggtcttc atttgttttc tatggttggt ttgtctgtga gaaggtcttc cggggaaggt
     5701 tcttcctcac ggagacggct tctgtctatc aaaccagcca caggagtgtc caaggcttaa
     5761 gggtgacaaa atagccaccc gtcccccttt ggcggttaca aggtcatgcg gagccatgcc
     5821 ctggcgtcca gggcgtctgc ggcggcggcc acttccgcct ttctcttgct ggccccggag
     5881 ccggttccaa tggcgtgacc atgccagaaa acgcgggatt caaaacggtt ttcggcgtct
     5941 ctcggccctt tttcttccgt ttcataggaa ggagtttcgg gcaggatgtc ctgaaggata
     6001 gcctggagtt cccccttggg attgatttcc ttgggatggc tggccgcgga atccagagct
     6061 tcatggagga cgcgcagggc cacagccttg gccgtttcat agtcggaatc cagggacatg
     6121 gctccgaaaa cggattcaaa tgtgttggcg atgatggaat ttttgccccg tccgccggcc
     6181 cgttcctccc cttttcccag ggcaatgtgt ttatccagcc ccaggataaa gccgtatttg
     6241 cccagattgg cgcggctgac ggtccgggcg cgcagctggg tcagttcccc ttccggggat
     6301 tggggcatgt ggtggtaaag gtactgtgtg acggccagct gcaggatggc gtctcccagg
     6361 aattccagcc gttcatacgc ccggcgcgtt tccggcttgc tgtccgtgct ggggtgggta
     6421 agggcctgaa cgagcagatc cggattgttg aaacggtaac ccagcttgtc ttccagcgcg
     6481 gtgtggttca tcccggaaat gctagggccg ccgttccgga aatgcaactc caaacggagg
     6541 gagcttcccg gcggggacgg aatttgtttt cccgcgtcat cccggccgct ccacagcttt
     6601 gacatatggg aggaggggtg taggatagtt tgcgaacgaa cgttggagac gatgatgaaa
     6661 aagattgaac caaaggagat tcaggataat gccgtacaat tgatcgggga cgattggatg
     6721 ctggtgacgg cgggttctcc ggagcatttc aacatgatga cggccagttg gggcggtttg
     6781 gggttcatgt ggaagaagcc tgtggcgttt gtagttatcc gcccccagcg ccataccttc
     6841 cgttttattg aggccgggga cgagctgacg ctttcctttt tcagccatga gtaccataag
     6901 gctctgaccg tgtgcggaac aacgagcgga cgggatacgg acaaggtggc cgcttccggc
     6961 ctgacgccat acgtgacgga gaacggcaat gtaagtttta cggaagcgcg cctggtgctg
     7021 gagtgccgca agctgtacgc ggagcctctc aacccggaag cttttctgga taaatctatt
     7081 gtgccggagt ggtattccgc cggggatttc cataagatgt atattgtgga aatcctgaac
     7141 gtatgggtga aggaatagcg ctccggaaat gccgcaggcg gacgcctgtc cgttgtttct
     7201 gtccgcgctt tttcagaggg gtgcaagggc gccatcctcc agccggaagg ctggcagggg
     7261 gcacggagca ttgtgcaacc agtccagatg ggtggtggtg atgaggctct gcgcgtccgc
     7321 tggaagcgac tgcagaaagg ctatccggcg cgtggggtcc agctccccga agacatcgtc
     7381 aatcaggtgg atgggcgtat gtcccgtttc ttccgtcagg agggaggact gcgccagctt
     7441 catggagatg gcggtggtgc gctgctgccc ttcggaggcg aattgagcgg cgtttctgcc
     7501 gttcagcgtg atgtccaggt catcccggtg ggggccgttc tgggtttgcc cgtaccggat
     7561 atcccggtcc atggaggcgc acagccgttc atacaggtct ccttcctccg aggcacggta
     7621 agcgatggag acctgttcct gccttccgcc gatattgcgg taggccaggg cgatatgggg
     7681 cgccagaagc gccagcagat tggcgcgcag gtttctcagt tccgtgccgt gcagggccag
     7741 ctgtcgcgtg taggcgtcca gctggagttt gtcccggtgc ctgtgcttga gcaggtaatt
     7801 gcgcgttttc agcgcccggc ggtagctgaa cagggccagt ctgtagcctg gatgccactg
     7861 gctgcccaga aagtccatgt atttccggcg ggcgtccgcg ccggcctgca ccagggaaag
     7921 gtcgtcattc cccatccata ccacgggata gctgtccgcc aggtagctgc gctggtcttt
     7981 acgaggctct ccgttgacgc ggaggtccgg agcgtccggc gcccacagga tgcgccggat
     8041 ttgtcccgga agttctccgc ggatgccgaa ggattgtttt ccgtgggaga ccagcggccc
     8101 ggtccgggcc gtgcgcgggg attgcagacg cagcaggaag caaacggcct ccagcaggct
     8161 ggtttttccc cgggcgttgt ttcccaggat gatggccccc tgctgcggaa tctgccagga
     8221 gaagcttccg tagcaacgga agtccattaa tttcagcctg gagatcatgg ggccgggaaa
     8281 ggggttattc cgcagggagg gagcggatgg cgtccatgat tttgtccgtc aggtgctggt
     8341 atgcatcttt tcctttggcg ttgagttccg ccggcgtgaa ggggatgggg tctcccaccg
     8401 tgaccgtgac ggggcggagc ctgggaaacc gggcgccgat gggaaaggct tcatacgcac
     8461 cgctgatgcg caggggctgc acgggaactt tcgttttgct caggatgagg ccgatcccgc
     8521 ccatgccgtc atggatttca ccatccggcg tgcgggagcc ttccggaaac acgagtacgc
     8581 gcttgccgga tttcagcagg cgtatcacgt gcttgaggct ggcggcatcc gggttttcct
     8641 ggtcaatggg gatagcctgg cagaggggca gggcccattt gaaaatgccc tggaacagcg
     8701 tcttgcgtgc gaaaaaatag atgccgtcct cataggaaat gcccagcatg ggcggatcca
     8761 gaaagctctg gtgattgctg acgatgagca cggggccgtc ttcgatgagt ttttcccggt
     8821 tgacgacttt ccaggagaag aaggctttgg aaatgctttt gaagagcgtg tagaaaaccc
     8881 agtagaaaga gttcatctgg aggagagttt ttgttggatg ttgtcggtga tgaaacggac
     8941 gacctggtca atcgtcatgt ctgacgtatc caccagaaga gcgtcaggcg cctgcgccag
     9001 cggggcggtg gcgcgggagg aatccttgcg gtcccgttcc gcgatggagt ccgttagtcc
     9061 ttccgcggcg cggcgcgcgg ccctcacttc ctcggaggcg gtgacgaagt acttgaacgg
     9121 cgtttccgga aaaaccacgg tgcctatgtc cctgccttcc atcacgacgg gttcccggcg
     9181 gttatattcc cgctgtcgtt ccaccagcag ggcgcgtact tccgggatgg ccgcaatggt
     9241 ggaaacgtgg tcgttgacct gccggctcgt gagttcttcg cccagcacat gctgtccgca
     9301 cagaactacg gatcggctgc cgtccttgcc gaaggagagg ggcacggacg gcagggattc
     9361 cagcacggcc gtcgtgtctg cagggttgat gccctgttcc agcatgtacc aggtaatggc
     9421 ccggtacatg gccccggtgt tgatgaacgt atagccgagg cggtccgcga tgagcttggc
     9481 gacggtggat tttccggagg cggcggggcc gtcaatggcg atggctggat gcatgagtgt
     9541 cttctgagtt ggaaatgggg gctaaagccg tttgaattgt agctttgagc cctgtggaaa
     9601 tcaagactgc gttccgaagg gcgttgcaaa aagagtttca ctttttcccg taaatccgtt
     9661 atacagcggg gcatggccca gctcaaacac ccgaagatgg tggacatcag agacattttg
     9721 gacgagaata cgcgtctgcc tgctctggtg gccgcgtccg ccgaaaagct gctgggcctg
     9781 gaacgcctga acaaggcgta cgacaagatc gtgcgcgaca aggaatccgg ttctcaggaa
     9841 aatttctttc agcttgcctc caggcatttg aatctgaagc ttcagctgcg cccgggggat
     9901 ctggaaaata ttcctaagaa agggcctgtc gtcgtcgttg ccaatcatcc gcacggcttg
     9961 tcggacggca ttatgttcgg ggagctgctt acccgcgtaa gggaggatgt ccgcatcctg
    10021 gtcaacgagc aactttccct gtgcggggag ctggatccct ggctcatcaa ggtggacgtg
    10081 tatgaggatg agaacgcccg gcgcaaaaac ctttccggca tgcgcaagat gatttcctgg
    10141 ctgcggcagg gcggggttct gggcattttc cccgccggca cggtctccag tttttccctg
    10201 gcccacaaaa gggtgacgga cgacccctgg aacgcgaata tcgccgccat tatccgcatg
    10261 acgaaagcga cggtggtgcc cgtatatttc ccggggcgca acagcctgtt gttccagggc
    10321 atttccctga tcaaccgcaa ggcgcgcgtg gcgttccttc cccgtgaagt gggtcgggac
    10381 ggccgccgga cacaccgcat tgtggtaggc aagcccattc cgttcagcca gcttggacaa
    10441 tatgattccg atgaggccat cgtctcccat ctgcgcctgc gcacctatct gctgggcaaa
    10501 agttatgaaa aaagccgccg cccccatgtg cacaaaaagg accggaaggc gaagatggcc
    10561 gcgctgacct cccccgtttc cgtgcaggat atgcaggcgg aaatcgacgc gctgcccccg
    10621 gagtgtctgc atgcccgtca ggaaaatgga gattgggacg tgtacgtggc ggatgccctg
    10681 caaatcccca atatccttat tgaaataggg cgtttgcggg aatatacttt ccgccaggta
    10741 ggggaaggtt ccggcaacgc ctgcgacctg gatacatacg acaaccatta caagcacctg
    10801 ttcctgtggg acagaacgca ccagaaaatt gtgggtgcct accgcatggg ggaaacggac
    10861 aaaatccttg cccgctacgg tgtgaaaggg ctgtacaacg gggaatactt ttccttttcc
    10921 tctgccgctc tgcgggtgct ggaccgctct ctggaaatgg ggcgcgcctt cattgttccg
    10981 gaataccaga agaggccgct ggctctcggt ttcatttggg agggcatcgg ccagttcatg
    11041 gcccgtaacc accattaccg ctacctgttc ggcacggtca gcatttcccg ggattacacc
    11101 aacctttccc gcgccctcat cgtctcctat ctgaaggcgc atgaaatgga gcccgtgctg
    11161 gtgaatgagg tgagggccta caatccgccc cgtaaagcgg acctgaagag gtcggaatca
    11221 tgcatcctcc ctctggggct ggcagatgcc caggggctgt cccaattggt cgctgatata
    11281 gaagaggatg gcaagggcat tcccgtattg ctccgccaat atctgaaatt gaacggcaaa
    11341 attctttcct tcagcgtgga taagcatttt ggcgacgtat tggactgcct gatcctggtg
    11401 gacattttca aaacgccgga gcgttccatc aagcgttatc tgggcaaaga cgcgtatgaa
    11461 cagctcctgc cttatatgca gcgggagaaa gaggaggaaa aggcggcgga ataattccgc
    11521 aggcgttttg cgtggctctg cccccggttc ttaacgggct agctgaagtg cctgtaacct
    11581 gggggaaggg cggaggctgt ttccgcagtc atttccccga tgacggtgag aggcgtttcc
    11641 ggaaaatgct ccgcggccag tcgtgccgcc cgttctcggt cgtcgggacg gaaggtcacc
    11701 agcaattcgt aatcctcccc atcccctaca gcctgggccg tagtgaaccc cgggcgcacg
    11761 ggaagaagct cttcatgaat acggaaccca agcccggagg cggccgccag ccgggggagg
    11821 tcggttccca gcccgtcgga aagatccatc atggaggtgg ctacgccgga ggcagccagg
    11881 atgcctcctt ccctgacacg cggcatgaag gaaagatggt ggccggtggg gaagctgccg
    11941 ccgagcgttc ccgttacggc aatcagatcc cccgaccgcg ccgtgctgcg cagtacggct
    12001 ttgcctgcct ccacttctcc ggtcagcgcc acgttgatga tgagcccgtc cgcgggcagg
    12061 ccggagcttt cccccccggc gatgctgatg ccgaactgcc gtgccagtcg ccccatgccg
    12121 cggtaaattc cttccacctg ggagatgggg cggtctgcgt gcacgaacag ggtcaccagt
    12181 gcgtgaaggg gcgtgccgcc catggcccct atatctgaaa cggcccgtgc cagcgccttc
    12241 ctcccgatca gttccgggtc cgttccgggt aggaaatgca tgccttccac cacgcagtcc
    12301 gttttcagga gcagctgacg gtttcctgac ccccggatta tggcgcaatc gtctcccggc
    12361 cccgtctcta gggacggatt ggaaggcatc aggggaagga ggcgggctac cagcgcatcc
    12421 tctcccgtct gccggatgct gggctcaggt tccatacgga agaaggatgc cgggatacag
    12481 aatgttgatg agagtggcgg tgttgaaaat gcaatgggcg atgatgggcg tccagatgct
    12541 gcccgtgtat tcgtacagca gcaccagtac ggctccgaag attgccaggg ggatgaacgg
    12601 aaccaggctg acgtggatga tgccgaagag gagggaggtg gtgatcaggg caaaccagac
    12661 gcccgtgtat tttttcatga tcgggtacag ataaccgcgg aaaataagtt cctccaccag
    12721 aggggcggcg atggcggcgc tgaagcagat aaccgtgatc aggggaatgt cgctggagtg
    12781 cctcagcacg gaaacaatag attgttcggc cggggcgttg gtggcgtgtt caatccactg
    12841 gaacaggccc gccttgtcca gcaggaagtg gatggccagc accagcagat aacctgccgc
    12901 cggagcgtag aggaggactt tcagggaatg ttttttcaat cccagcgcct ccatccggcc
    12961 cgtatggaac atgcgcacca gcaggacgaa ggccagaatg agattcaggc atgttccgtt
    13021 gaaaaccttg tagctgtcca gctccatgtt ggttgtgctg ggcggagcgg aagcccccgg
    13081 aggcgtggct gccgccaaca gggcccccag gctgaagtaa agcacaatga tgccgcacat
    13141 ggcgatgtcc agcatgtcca gatggcctac gggaatacgc catttcagcg gatgccgggt
    13201 atcaggcgca tgattccggc ggaatatgtt taccgccacg gcgaggaaca tgacggtaat
    13261 caggatggaa gagaacaggg taatgagctg gtccgttaat tcattcatgg aataggcatg
    13321 tgtggaatgg gccgcccttc cagagtaccg cgccggaaga aaagctcctg tctctttagt
    13381 ggaaacacgg tgattttcca gcagaaaatg agctatctcc cctcattccg caaaaaaaat
    13441 catcaggaaa gggaatgaat acttttcaag gaaaagcggg tatgctacgt ttccaacgga
    13501 ttatcgccat gaatattcaa cccaaaatca cgcctgtgct ggaccccggc ttcgtgccgg
    13561 ccgttctttg gaatcaagct tttgaggcca aagccgccgc cgatcccgct tctcatcaag
    13621 tggatatcgc tctgacccgc aatgacggga cctgtttccg ctggtccggc aagcttcttc
    13681 cccataccgg agaaaacatt gccctgaatg agacctatgt ggagcgcatt gtgaaattcc
    13741 tgctgtggca gaagggcggc aatatcatcc tcgtcgccgg ggatgacgct attgcggaca
    13801 tgctggcctc ccgctattgc aaaggcggta tccgcgaatt cgactgggat ttcatcggca
    13861 agaagattta cggctccccg attgaagtga aaaaggtttc cgtggaagaa cttcccgagg
    13921 aatattccgg ttccatgaca ctgggccgca acctggacgg ctgccgcatc ggttttgacc
    13981 tgggcggttc cgaccgcaaa tgcgctgccg tggtgaatgg ggaagtggtt tattccgagg
    14041 aagtggtctg ggacccttac ttccagaagg acccccagta tcacattgac ggaattcagg
    14101 attctctgga acgcgccgcc gctcatctcc cccgggtgga cgccatcggc ggatcctccg
    14161 ccggcgtgat catcaacagt gaagtacgca cttcctccct gttccgcggc gtcagccagg
    14221 aagacattga aaagactctc ggcaaggtat tccgcaccct ccagaaggaa aaatggaaca
    14281 acattccctt tgaagtggtg aatgacggtg aagtcaccgc cctcgcaggg gccatgggca
    14341 tgaacgataa cgccgtgctc ggcgtggcca tgggcacctc cgaggccgcc ggctacgtgg
    14401 atccggaagg ccacatcaag ccctggctga atgaactggc tttcgcccct gtggactact
    14461 cggaggaagg cggagtggac gaatggtcca aggatatggg cgtaggcgcc ctttacttct
    14521 cccagcaggc tgtggcccgc ctggcccccc gcgccggctt ccagtttgaa ggaatgccct
    14581 tcccggaaca gcttaaaaag gttcaggccg ccatggcgga aggtgacgaa cgcgcccgca
    14641 aaatttatga aaccatcggc gtgcacttcg gctatgccat tgcccattac gccaggttct
    14701 atgatatccg caacctgctc ttcctgggcc gtgtggcttc cggagacggc ggccagatca
    14761 tcattgacaa ggcggaagaa gtgctgcgca ccgaattccc cgagctgaag atccagctcc
    14821 gcgtgccgga tgaaaagacc aagcgccacg gacaggccgt agccgccgct tcccttccgg
    14881 ctctctcctg acgggattaa tcaaatttgc gggaggggcc ctggctgggg tccctccctg
    14941 ctcatattcg gaggggggga cggacaatcc gcgccgttat tcctccttcc ttttttctga
    15001 ttcccgttcc gccgccctgc gttcatgatt ccccagcaag aaaacatcgt caattgcccg
    15061 gcgtgcggac agcccatgga tgtttccctg ctgcctccgt acgccaacgc catttgcccg
    15121 ggctgccagg cgctgacgcg tgtaaaaacg cgcatggggc cttatcgtat taccggaaaa
    15181 ctgggaaaag gcggcatgag cgtggtgttc cgggcggagg atgccgttct ggggcgggaa
    15241 gtggccctga aagtgctgaa tgatacttat gccggggatg ccctgcgctg cgaacgtttt
    15301 gaacgggagg cccggatcat ggcgcgggtt tcccatgaaa acctggtgca gctttatgcc
    15361 gtcggacgtg accagggcct tttttacatt gccatggagc tggtggaggg caatggcctg
    15421 gatgccctga tcgctgcgga ggaacgtgtg ccggaagaca gggtgctgcg cctgacgctg
    15481 gatatcgtgc gcgggctgga cgccgcatgg aatgcgggcc tcatgcacag ggatatcaag
    15541 cccgccaacg ttcttttgtc ccccgacgga gccgccaaaa tcgtggattt cggcctgtcc
    15601 ctgcttcata gtgaatccga catggagcgg gaaatatggg ttactcctta ttacgccgcg
    15661 ccggaaacgc tgttgcgagg agaggaggac ttccgtacgg acatgtacgc tttgggagct
    15721 accatgtacc atttgctggc aggtgcgcct ccccgtgtgg acgcttccca gtcttccgat
    15781 attcttctgg agaacaagaa aaatctccct cgcctggagc aggtggtgaa cgacgtttcc
    15841 ccaatgacct gtttcattgt ggacaggctg atggcttttg acaaggaaga caggttctct
    15901 tcctatcccg aattgatgga tgccgtggca caggcgcagg aggaatacgc ccgggccatg
    15961 caggaatccg gtctcacctg ggcggaacgg cgcgcggtgg aggcccggcg cgcacggcgc
    16021 aggaaatgcc gcatcatcgt tgcctcctcc attgctttca tagtggcgct ggcgttgggc
    16081 gtttggggat ggacgaaatg gcgggaggag ggagctcctg ctcccgcaac tccgccttcg
    16141 cccatttctc ctgcggcgga agcagccttg gaagacaggg gggcggcgga atcgcggctg
    16201 gagcgcagca tccgcttcgg caaactgttt aacgaagccc aggaatccct gaaccggggg
    16261 gatctggtga aagccgccgc catgttcggc gatttggcgg ctcagccgga ctgccccctg
    16321 tccacatccc tttgggccgg tttgaaccag gttctgtgca tgtgggtgcg cggtcagttc
    16381 ccggaagggg tggagcgtct ggaagaactg ggcagaaaaa tggaagcctg cggggaccct
    16441 gaggaactgg aacgttccag ggatattggc aacctggtca tgtacctgag ctccgcggac
    16501 tggtcttccc ggatgccggg gctgcgctcc aattctgacc tggccgtgca ttattatgtg
    16561 ggcatggcgc tgaaatcctg gtactttgcc gggagatgga cggaatacgg cggctttctg
    16621 gaccgggtgg ccgctgatgc ggcggatgac cgggacagga acgtccgtga gctggcttcc
    16681 gcgtggctca gcaacctgga gaggtatacg gaccagtacg tgcgcttgaa acgtcttcag
    16741 gacatgccgg aaaaaacggt ggctgaagta gaggccaaac ggtccgcggc ggacttcctg
    16801 cgggaacaga tggtcagcgg ggaagttcct tccatgtctc cggcttatgc cgcgctggaa
    16861 ggaatactgg atcatttgag gatgcagtac caggctgcgc gggactggga ggctgcggaa
    16921 gccgtgaagg cgtctgaaag gaaaaaggcg gaagagaggc aaaaaaaact ggaggaggaa
    16981 aagaggaggg aaattctgct ggccgcccaa tccagaagtt atgaagacgt ctgccgggaa
    17041 gcggagggca tcatgaagaa aaccggtgat tatgagaaag tttccattgt ttatggagag
    17101 gcggaaaaag tcatttcctc tcctgcggtg aaagcccgcc tggccatgcg ccgggaaatg
    17161 acggaccaga tgcttccgtt gttctcccgc atggagagac ttttgcccgc actgctggaa
    17221 aagaaaccgg ggaaaactct ggtgctcaga gatggctcca gggtgcgcct gaaaggaatg
    17281 aaggggcatc tgctgaccgt ggagcctcag gacagccggg aaggaagcgg tgcgtaccag
    17341 gtcagttgga acgagcttcc gttcaactcc ctgcatgctc tagcgcggga atgccgccag
    17401 aagcagcctg cggaattttc ttccctggcg gatacgtatt ccaagccttt gcttnnnnnn
    17461 nnnnttgctt atttttggca ggctgacggg aaccatttcc gccgcacagc aggaaaaagc
    17521 attgcttcag atggaccgct ctttcattga aaaatggaat ttatggatga acagtctgga
    17581 ggcggaggaa gggctgccgg gagacgatgg aaaatcaagt tgattcagga agggaataaa
    17641 gtcccttgaa aggcggcgga ttgctgccgc cggcaggttt ctgcccccgc cgcgtgcttt
    17701 gccggctgcc cacgggccga aaaaaggaaa accgtccggg caaatgcctt tgttccggat
    17761 ttggagaaaa gtaatggtga tgaagtaaaa tttgcttgcc tagggtactt cgtgcgggtc
    17821 tcatagccgc atgagactga tcagctttct gacgctggca tgcgtggccg gcttcctctc
    17881 ttcatgcagt tgtgattgtc ataagcccgc ggacttcgct ctgaaggatt accagcctac
    17941 ctccagcaag caattccgtt aagttcccta tatgtttgaa atccgcgaaa aaccggaaat
    18001 ggtggaacgg gccatgctcg tttccattta cttcgatccc tccgaggctg gggaaaaaca
    18061 ggccatgctg gatgagctgg aagaccttgt ctccaaactc gg
//
LOCUS       DAWEMX010000044        18032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1292_length_18032_cov_9.269678, whole genome shotgun sequence.
ACCESSION   DAWEMX010000044 DAWEMX010000000
VERSION     DAWEMX010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18032
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_18032_cov_9.269678"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1056..1065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6156..6165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6742..6751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7794..7803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8338..8347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9813..9822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13556..13565
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4327 a         5654 c         4539 g         3442 t
ORIGIN      
        1 tgataggaga actgggcgga cgcctcgtgc aggggaagat tccggaatct ggaagaaccg
       61 cagaacaggg ccagcctgtc cagaacggga aggccggtca gcatgccgcc ctgcaacctg
      121 gcgagcccgg ccacatgggt tacgccgtcc cgtccgccgc gcaggtctat cccgccgctt
      181 acgctgccct ggacatgctt tttccaatgg ccttccagga cggaagaggc ggggacgccg
      241 ttcaccacca tattcgccgt ccaggaggag gaagagccgt cccagtcccc cttgatgtcc
      301 agataaccgc cggcagccgt agtcaggcgg caggacggaa cgttcaccct cctgctggca
      361 tggttaaact gcgccgtgcc ggacatcagg gaaaactccg ggcatccctt gaaaggaagg
      421 agcagccttc ccccctgcat ttcaaacctg tactggctgg aaccggcgtc ataggcgctc
      481 tccacgcggg tcccgtccaa aacatacggc cggccggcct ccccatagga aacggacagg
      541 gaatcaatgc tccccttttc cagacggaac gtatccggca gaatattctc ttccgcccag
      601 ctcctggccc cttccggctt ttcccggaaa cggaaaggca cgggagcgag ctgaagcttc
      661 agctccgcaa tattgacgga ggtaatgaca aacgcgcgct tcagcaactc ccagcggctg
      721 aattccgtct caatgttcct ggcctgaagg gaagtcaaaa attcatgccc ctccatcgtt
      781 acgccgccgc accggatgcc ggaaccgtcc cagtcaagag ggtctatcct ggcggtctcc
      841 acattcatat catcaacggc ggactgttcc agcatggcta aaaaaccatc gctcttcaaa
      901 taggccctga cgtacagata ggatcccgcc accacagccg ccgccagaac aagcatcccc
      961 agcacgcccc acagcagcag ctttttccag acgctgccgg cacggggctt tttcctgcgt
     1021 atccttctta ctctgggcat gacgccagcc tatatnnnnn nnnnnatatc aaatccggct
     1081 tcggtcaagc ctccggattg cctcccggcc tgccgcaaac tgcggggaac gggcatccgc
     1141 gcagcaggcc ttccccgcct atcgcggacc gtggtacacc ttatgccttc cggaagccat
     1201 ggtgatgaaa atcaggaaca gggcataagc cgccagaaac cccatgggaa tgaccgctcc
     1261 atgttcccca cgccccagcg cttcaatggg cgcggcctgc ttcacccaga tattctgcgc
     1321 cagccaggct aggaaaatcg ccagcagcag cgtaggcgtc acaaacttca tgatgaaacg
     1381 gtaaattctg ggaatgcgga tgacggaacc gtcctccaga tccttcaacc cccggtccac
     1441 ccccagtttc caggaaaaca gaatcatcat caccatggcg ctcacaaaga agcacagcgt
     1501 gcccagaaag aaatcataag tatccatcgc cagcagattg ccgctgtacc aggcaacgat
     1561 gaacgttccc gtagccgtaa aaaagccctg caccaccaca gccattttgc gtttcagccc
     1621 catgaattcc tcaataaagg acaggcccac ctgcatcatg gaaatggagc tggtcaccgc
     1681 cgccaccgtc agaagcagga aaaacagact gccgaatacc acgctggacc ccattttggc
     1741 aaaagcctgc ggaagcacca tgaaacccag cgcgaaggtc ccctgcccgg cggctccggc
     1801 cactcccagg aacgccacgg cggcgggaac cgtcatcatg ccggcaatgc ccacctcgca
     1861 cacttcattg gaagcagcgg ccgaaaagcc gctcagcgcg atatcctcct tcttttttac
     1921 atagctggca tacgtcagaa tcagccccgt tcctacggaa aggctcagga aaacctgtcc
     1981 ggcagccgca atccaaagct caatgttttt caggccgtcc cacagggtca cctcagtcag
     2041 cctccgggtt cctccggaca tttccacctg ccgcacggct tcatccatgg ctcccgcctg
     2101 ggaggccgag accatggaaa ccgtcttcca gtccccgctg ttccggtcca gctcctccac
     2161 cagcaccttg ccgggattcc acatataccc cagcccctgt tcgatgctgc ggtccggata
     2221 agaagggtcc ggcgtgccga tgcacaaaat gcgcaccagc agcaccaggg aaatcagaag
     2281 caacaacggc atgaaccagc gcgaaaacat ttcgatgccc ttgctgatgc cccggtaaat
     2341 aatccccagg ttcaaaagaa tgaccccgct gacaatccag agcagcccgt tgtccgacaa
     2401 cagggcgctc ccgtcgccgt acatgcccgt gaaactgaaa aaagtctgcg ccgtcccctc
     2461 cgccgtggca aaaacctccg ggcaggtgag catcttccag aaatagccgc agcaccacgc
     2521 ttccaccacc atgtaataaa aggccacgga agaaggcccc agaaccgcca gcacgcccag
     2581 gaacttccat agcctggacc ctttggtcag gtaccagaat acgccggggg cgcaatgggc
     2641 gcccatctgg ccgccgcgcc gcccgatgga ccactccgcc cacgccacgg gcacgcccac
     2701 cagaaccagc atgaggccgt aagccaccat gaaggcgccc cctccatatt gggcagccag
     2761 ccccggaaag cgcagaaaat ttcccagccc gacggcactg ccggccacag cgaggatcac
     2821 gccaattttt ccggaccact cgtcttgttg cgcctttgag gatgacatgg agggaatact
     2881 gacaaaaaaa gcttcacttt caagagaaaa caggctcttt tggaacgaac ggggtatcat
     2941 tgccgcttga ctttcgtccg ttttttggaa tcattctttt agaaccaaac caaaaaacaa
     3001 ccatgcttct cataggaaaa ccggcacctc atttcagcgc taacgcagta gtaaacggta
     3061 ccatcgtacc ggatttcagc cttgaccagt ttaagggcaa aaaatacgtg atcctgttct
     3121 tctacccgaa ggatttcacc ttcgtgtgcc ctaccgagct gatcggcttc caggaagccc
     3181 tgggcgaatt cgaaaagcgt gacgtggccg tcgtcggctg ctccacagac agcgaattct
     3241 cccactgggc ctggatcaac accccccgca gtcagggcgg cattcagggc gtgacctacc
     3301 ccatcgtttc cgacatcaac aagaccattt ccgccgatta cggcgtgctg gccggagacg
     3361 aggaaattga cgaagacggc aacgtggaag tcaacggaga actgatcgcc taccgcggcc
     3421 tcttcctgat tgacaaggac ggcatcgtcc gccaccagct catcaacgac ttcccgctgg
     3481 gccgctccat tgatgaagcc atccgcgtgg ttgacgccct ccagcacttc gaactgtacg
     3541 gggaagtctg ccctcttggc tggcacaagg gagacgcggc catgaccccg tcccacgaag
     3601 gcgtggcctc ctacctcagc aagtaagcag ccccccggca atatttcaag gccgctcccc
     3661 ttccggggga acggcctttt ttcatccccg ccttcccctc tttcccaacc aggcgctata
     3721 tccggccatg catccgccgc ctttcctgcg ccggaaaccg cccccccttc tgcaagatga
     3781 acctgtgcgt ccgccgcacc tgaataccct gccctttccg cagccgaagg aactgtcata
     3841 aaccgggggg aatacgcgcg cgccccttga gccggggaaa agcgtctgct acatccttca
     3901 tatgaatctc atggcagacg catcctccag ggaagatatt tggacaaaaa ccatggactt
     3961 cctgaacatg ccggactggc tcaagctgga aatggtgcag tccgtgggaa tcaaaatcct
     4021 gcatgtggcg gtatggctgg tgatcagctg gatactcctg aaactgttct gccaggtgct
     4081 ccggaaaatc actgcgttga aaatgtcgcc ccaggcctcc cgcctgacgg taaaaatcgt
     4141 caaaaacgtc ggctacatta ttataggcgt ggaggccttc gccctgatgg ggttcgatat
     4201 cctgaccctg ctgggcgcgg ccagcatcat cggcgtcgcc gtaggcttcg cctcccagac
     4261 ctccctgtcc aacatcatca gcggcctctt cctggtaggg gaaaagcaga tcaacctggg
     4321 ggatatgatt gaagtgaatg gaatcaccgg gaacgtggac tccatcaacc tgatgtccgt
     4381 ccagctccgg ctgccgaaca acaccatggt gcgcatcccc aatgaaataa tcatcaagaa
     4441 tccggtcagc aatatcaccc gtttttccac gcgccggtgc gacctgagcc tgggcgtgga
     4501 ctataactgc gacattgagc atgtcgtgaa cgtcctccgg gaagtggtca aacaaaacaa
     4561 attctgcctg gatgaccccg cgcccctcat ctcgttttca gggttccagg actcttccct
     4621 gggctttacc gtaggggcct ggtgccggaa agacaatttc caggactgcc agaaaacgct
     4681 cgcccatgac atcaagcgcc gctttgagga agaaggcatt tccttcccct tccccacccg
     4741 cagcctggag agcagaagcc ccatcaaggt ggaaatctcc ggacctccgg agaaaaatca
     4801 ataaggaatc tctttttgct ttctttttgc cccgtgaaat ttcaatcttg tctgcgtaaa
     4861 atccgcatct atgtctgaca catccgcacc agcctatcga agaatccttc tgaaactcag
     4921 tggggaagcc ttgcgcagcc ccggcagcat ggacaacatt tccccggaaa tcgtcgcccg
     4981 cattgctgaa gaaatcgccc aggcgcaccg cgccggcgtc caaataggcc tggtcgtggg
     5041 cggcggcaat ttctggcgcg gggcgaaagc cagcgtgcgc ggcatggacc gcgccacggc
     5101 ggattacgtg ggcatgctcg ccaccgtgat gaacgccctg gcccttcaaa gcgcgctgga
     5161 acgcgcgggg gtcccctgcg tggtgcaatc cgccattgaa atgaaaaatg tcgcggaatc
     5221 cttcatccgc ctgaaggccc gctcgtatct ggacaacaat aaaatcgtca tcttcgccgc
     5281 cggcaccggc aacccgttct tttccacgga caccacggcg gccctgcgcg ccagcgaaat
     5341 caatgcggaa gtggtcatga aggccacctc cgtggacggg gtgtatgatg cggaccccaa
     5401 aaaagtctcc ggcgccaagc gattcgacac catctcctat caggaatgcc tggaacgcca
     5461 gctcaaagta atggactcca cggccttcac cctgtgcatg gacaacaaaa agcccatcat
     5521 cgtattcgac atgcatacgc cgggcaacat cacgcgcgcg cttctcgggg aaccgatcgg
     5581 caccaccatc agctgaaccc cgcattcctt ttcaacccac caaaaacact tagcacccac
     5641 catcatcctg gacgcaggaa cattattatt ggaaacggag gaatccatgc tcaaggccgt
     5701 ggacttcgcc aaacaggaat tctccggcgt acgcacgggc aaggcttccc ccggcctcgt
     5761 ggaaaacctg gacgtgcatg tctccagcta cggctccgtc atgaaactga agggcctggc
     5821 cgtcatcagc acgccggaac cccgtctcat cctcatccag ccctttgatc cgagcaccgt
     5881 ccacgacatc ggccgcgcca tcaatgaaag caagctgaac ctcaatcccc tggtggaagg
     5941 ccgttccatc cgcctgccca ttcccgccct cacggaagaa cgccgcaaag accttgtgaa
     6001 gcttgtcaaa tcccaggcgg aagaagcccg tgtgcgcgtg cgcggcgccc ggaaggccgc
     6061 catggattcc gccaaaaagc tcaaggcgga aaacatcgtt acggaagacg gccagcgcga
     6121 tctggaaacg cagatccaga agctgacgga caaatnnnnn nnnnngtgaa ggagattgac
     6181 gagctggtgg ccgtcaagga aaaggatatc atgaccattt aacccatccc caaccatttt
     6241 acgacaatga cagacaacgt aaccaacttc ctcgcctccg gcatcgaaat caagggaacc
     6301 atccgtttcc agaacgacat gcacatcgac ggcaaaatcg aaggcgaaat catctccgac
     6361 aagggaaaag tcaccatcgg tgaaaccgcc caaatcaagg gagacatcac tgccggagac
     6421 gtccgcatct acggcaacgt ggaaggcaag gtcacctctg accgctgcga actcaagcag
     6481 caggcccgtg tcgtcgggga catgaagaca cgcagcctcg ccatggagga aggcgcccac
     6541 ctgctgggcc gcacggaaat cggctaaaaa acagccgcct ctattcttct cttggcgtgc
     6601 ctgccttgta cgggccgctt ctccaaaagc gtaaaagcgc gccggggttt ctcagggggc
     6661 ggcgaaaatg aggaacggaa aaaaggtacc gcactttttt ccaaataccg ttcccgctcc
     6721 ggaagcggaa ttgctgggaa cnnnnnnnnn ngttcttcag gaagcggacg gatgccgttt
     6781 tacacaaata ccatccggca gaaaaaccgc ttctttcttc tgtccgaacc atcgccggat
     6841 atcacgcagg cgcatcgccg cctgcgtttt ttattgagtc tccacgctct ctccccgcct
     6901 ccggcgaaac atccgtcacc ggagcaggcg gaacttgccc tggtgcggaa aagccccgcc
     6961 gagcaaactc tccaactccc gtgaacacgg aaaaacgttt ccccggcagc cacagacgcc
     7021 tgtcttccga agccgtgcgg caaaaaaact cttctgccgc gaaacagaag atacgggaat
     7081 aaaaacggaa aacgtcccgg attcttgaaa ggccggcagc cattgccgca ccggcaccgt
     7141 ttccggaaat ttgcctggcc acgcaaaaag ccgtccctat cccccgcctc tttatctgga
     7201 gctttatatt tgggggcttt acaaaaaacg cgtgaagaac tacattcttt ttgtcaaata
     7261 tcagaccatc aaatatcata ctttattcat ctccgcctat gagcaaagag agcgtcaata
     7321 tccacgtaaa caactactgc cggctacggg acaaacaata tgccgtctat acggaatacg
     7381 ccagaaaaca cgggttaacc accaaagagc tgtttattct ggatatcatc tatttcgctc
     7441 agggcggctg cacgcaggcc gggatttgcg aacgcctctc cgccacgaag caaaccgtca
     7501 gcgccgccat caaaaaattc tggaagctgg ggtacctttc ttttgaagaa tccaaaaccg
     7561 accggagaat caggttgata cgcttcaccg cagccggaaa ggaatacgtg gaacgcatca
     7621 tcccccctgc cgcgcaggcg gaaaacgacg ccatggcgga attgagcgga gaacagcaag
     7681 cggaactggt ccgtttaacc gacctttttt cagaacaaat gcgtctgaaa tttcaatcca
     7741 tcaacaaaaa cacgctatga ttatcaatac aggaggacgg acggacaccg tccnnnnnnn
     7801 nnnacacccg ctggctgctg aagaggtttg aagaaggcta tgtcctttcc cgcaatcccc
     7861 tgttccaaca taaggtgacc cgttatgaac tgacgccgga ccaagtggac tgtgttgtct
     7921 tctgctctaa aaattatgcc cccattcttc cgcatatcaa cagcatcacc gagcgcttcc
     7981 atacctactt ccactatacg attacggcct acggaaagga aatagaaccg ggtgttccca
     8041 gcattgatga aagcatggcc acgctggcaa aactttcgga agttgtcgga gcaaaccgca
     8101 tcgcatggcg gtatgacccg gtattgctga caagcgaata taccatcaga aggcatctgg
     8161 agacctttga acgcatggcg tccttcttat ccggccacat cgaccgctgc attttcagct
     8221 ttgtggaaat gtacaggaag ctgcaaacca acatgccgga actaatcccc ctcacggaaa
     8281 aagacaagga ggagctggcg gccgggctgg gagcaatcgc ggccgaatat aaaatccnnn
     8341 nnnnnnncca ttcaaacctg cggaaccaat ggcgattaca cgcgttacgg catttcatcc
     8401 tccggctgca tgaccctgaa cattctggga caggccaacg gcgttcaatt ccgcaacctg
     8461 aaacatcaag gaatgcggca gggctgccat tgcattgaga gccgggacat aggagcgtat
     8521 gacacctgcc ttaacggctg caaatactgc tatgcaaata cgaaccggca aaaggcccgt
     8581 gaaaattaca aactccatga cccggactct ccgttgctgc tggggcatct gctcccttcc
     8641 gataccgtgc agcagggaat gcaaaggagc tttttaaaac catgaacgct gatgcaatca
     8701 aagtaaggga aattcaggta aagagcatcc tgtcaacgtc aaatctgcct gtggccgatt
     8761 actcggtcaa tccttacgtg ggctgccccc acgcctgtaa atactgctac gcctcgttca
     8821 tgaaacggtt caccgggcat ccggaaccat ggggaacctt tctggacgtc aaatactggc
     8881 cggaaatacg caaccccggg aaatacgcgg gaaaacagct gtttatcggt tccgtgaccg
     8941 acccttacgg acctcaggaa gaaatctaca agaggacaag ggcgcttctg gaacagcttc
     9001 aaggcagcgg cgcggaaatc agcatagcta caaaatccga tttggtgttg cgggacctgg
     9061 atttgataaa aacatttccc catgcccgcg tttcctggtc cgtcaacacg cttgacgagc
     9121 actttcaggc agatatggac atggcggtga gcatctcgcg gaggctggcc gccatgaagg
     9181 ccttccatga ggcaggaatc cgcaccacct gcttcatctc ccccattttt ccgggcatta
     9241 cggatattcc ggcgattgtg gagcaggctg aagacaaatg caacctgatc tggctggaaa
     9301 acctgaatct gcggggcagc tacaaatccg taattctgga gtacatcaac aaacgctatc
     9361 cgcacctggt gcccctctat cgggaaattt accagaaagg cagccgcggc tactgggaag
     9421 gactggacgc agcaatacgg cagctggcgg aaaaacgggg gttgccgtac ctgcgcaatg
     9481 acgattccat gcaccggccg ttcaacgaac cgccggttat cgtgaattac ttttaccatg
     9541 agcagatcaa acgttccgcc atgaaaaaag gggcgcttcc gaatcctccg cctcccgcgg
     9601 cggcctccag ctattgatgt caaaaacgcc tgataaccct gcatggactc ccaacagcaa
     9661 cgcagctggc cggcacagaa attccccctc ccgagcgatt tgccttagcg cattgggatg
     9721 tgccgtttcc aaaaaaatct tccgcttcat cacgcatggc tttcctcctg gcgaaaactg
     9781 ttttcaacga cgcccggcag aagctcataa atnnnnnnnn nngcccgctt ttttctcaaa
     9841 gaacgccttg agctgcagaa tttttttcca ccggtccacc gtaaacggat gcactccata
     9901 ccggattgtc acatctacaa accgtttttc caaaaggcag aaacctgtcg gcatggccaa
     9961 agcgtcacat aactggacca ggcggtcgta gtcgtcatag accgcgttct ctacaaaatc
    10021 tttcatgaac aggtaatcct ccccactcac gtcaaacgtg ccgatggaag tattgatatc
    10081 ctgtatcata aacgcatggg aaatacaaat ctgcgccgcc tttttccagc ctcttgccgc
    10141 acaatagcgg tacccgtcta tcaaatgctt ttcggaactt actccggcat accgtccaat
    10201 atcatgcaac agccctagga cataagcttt ttcaccggat aaccctttac agcgcgaggc
    10261 tatattttta caggcttccg ccacatagcg ggaatgttcc gcccagcccc ccgggttgga
    10321 ttcatacgct tccctgattg cccgttccgc agttttcctg tccggctcca tctcagtcat
    10381 ggctgccact atagcgcatc accactgaaa taaaaatcac gattccgcta cacccctccc
    10441 ccagccttca gatgatgtaa cgcaaaaata ttccttttta tttaatcctg cctttttccc
    10501 ggacagggaa aaaggaaatg attttccggg gaattactcc ccggcaggcg gacgcacgat
    10561 ggaccaggaa cgcggctggg cgggatccag caccttcctg ataaaggcgt tcatccgggc
    10621 cagatccata ttcctgatag cgtccgtctg ctgttcaaac gcttccagag gcagaccgaa
    10681 caggatatcc agcgccagca tctggcacaa agttccattg gactgcatgg ccagcagacg
    10741 ggaagacagg gcggccgccc tggtgcgttc cagctcctcc tccgtcatgc cctgttcatc
    10801 aatcatctcc agtgtttttt ccagcctccg cccggcttcc tccaattgtt ccggggaagt
    10861 gcccagatag aagcagatgc ctccggcatc catgccgatg aacagggaag aactggcata
    10921 ataggccagt ccggcctcct cccggatctt ggtgaaaacg ggaccggcca tatcactgca
    10981 ccaggactgg aacagcagag cctgggccat ctccggggaa gccacgtcca cgcccggcac
    11041 ccccactacc agcacggcct gctgtttatc cagcacggcc acttcccggc cggcccgcag
    11101 cgcgggcgtg ggaatcaggg ccggaggcgt tcccgccctc atgcctccca gatgtttttc
    11161 cagaagaggc aggacctcct ccttcctgac atctccggaa atgcaaatca cggcgttgga
    11221 agcgcagata atgcgttcat actgtttttt gatgtcctga accgtcagtg aagacaggct
    11281 ttccggcgtg ccggaagggg aatttccata ggaaacatgc ccgtaagcca ccttccgctc
    11341 ctgccggaac gccacggaaa gagggtcttc catatcctcc tccgcatccg ccacaaaaga
    11401 ttccttttcc ctcagaaagg cgtcttccgg gaaagacgga ttcatgacca catccgccat
    11461 cagctccaat ccggcgtcca ggtcttccgc cagaacctga catcccacgc tcagggaatt
    11521 gttaccggtg gacgtgttga tggctccccc gatgtcctcc agaaaacggg ctatatccgc
    11581 cgcggaacgg gtggaggttc ctttcagcag gcactcggac atcaggtccg tcaccccggc
    11641 gtcatgctca ttctccgcac ggcatccggc cttgaacgcc atgcaggcat aggccagcgg
    11701 caggcgatgg tcttcacgga tcaccacctt catgccgttg ccaagaacat gctcgctcag
    11761 ggccgttccc gcagaggcgg aggccctttc ttcatccccg gcgttgctgc ccggggggtc
    11821 caggctgact tccgtcacat tcggggaaga cagccaggtg gaacaaacgc ggtgcaaatc
    11881 ttccgtagtc acccgttcaa tagcttcctg ccattcggaa gacaggttca aattgcgcga
    11941 gccgaaccaa tccgccccca tttccgaggc caccccggaa gcggaactcc tgcggcgcag
    12001 gcgactgacc cgggtctgtt tgcagactct cttcagatcc tccgtaaaat cctcttccgc
    12061 cagattgcga agctcctgaa gcaccaggtc ccgcaccttg tcgcgctggg cgcggtccac
    12121 gtccatgctg atggtaaacg ccccttcatc cgtctcagac tggttggaat gcacctcaat
    12181 gctgtacacc aggcccaggc ggtcatggaa tttttcataa aaacgggcgg aacgtccgcc
    12241 tcccagaatg ctggacagag cggaaagggc tggcgtatcc ggatgggcgg aacagggcag
    12301 gcgccaggca aggtgcagct tggagtaagg cacggcaaac tccttccgca ccctgcggga
    12361 accgaactgg tgaggttcat ggggaatggg ttcccggttc aggggacggg atttcaggtc
    12421 ttccaccagc aattccagat gggaaagaat ctccgcggca tccacgtccc ccgcgatgga
    12481 aagaaccacg ttgttcggaa cataacggct ggcatggtaa tccagcacgt cctgccgcgt
    12541 caggcagtca aaggcggcgc gctcacccag aacgggccag cggcggggat gcttgagata
    12601 cagcgtctgc atcagaagct ggtaggccac ggaatcggga tcatccgcat acatggccat
    12661 ctcccggcgc acgacctcct tctcccgctc catctcgtct tccgggaacg tggggaaaaa
    12721 tacaagctcc gtcagaagat tcaggaaaat ctgccaggac tccgcagggc cgtctatata
    12781 gtacacggtg cggttcacgc tggtgtacgc attccagtgg ccgccgcgtt cctgcacctt
    12841 gcgggccagt tcctgcccgg agaaatgcgc cgttccctta aacaccagat gctccagcaa
    12901 atgggaaagt ccgctcccct gccagtgccc ctcattcatg gaccccgtgc ctacccagta
    12961 ctggagggac actacgggat gagacttgtc ctccttaatc aaaaccgtca aaccatttcc
    13021 aaaacggcgc gtcacaggtt ccttggctga actcatgttc cctaccgtgc ctcaagaagg
    13081 caccccatgc aagccggcgc tccgtacgtg cccccggact gtcaaactta ccccggaggc
    13141 taaaagcgcg tcctcatcaa aaaaaaccgc cctaaaaaga atcctctccg cgcatttttt
    13201 tgaacaaata tcactccctg ccgctctatc tgtcgtagaa cggcagctct tctgccgctg
    13261 cattaccaac accacacaac ctcacatatc attatatgaa aaaaatcctc gctgtagcag
    13321 cctttgcttt cgccttcagt gtcaacggtt acgctcagga agccgccgct ccggcggagt
    13381 cccccgcccc cgctgcccag aagtgctgca agaaagacaa aaagtgcccc aggaaatgcg
    13441 ccaaggaagg caaatgccca gaaaaatgcg gccagccttg cagcaaaggg gaaaagccct
    13501 gcgactgccc caaaaaggga gaagccccca aggcctgacc caacggaccg ttaaannnnn
    13561 nnnnntgccg gaaccttccg gcaagggttt tctccgccgg aagagggcag ccgcgcccct
    13621 tccgttgact gcgaaattac ttgatcttaa ccttcaaccc attttataaa tcgtcatcat
    13681 gaaaacagct tccattctta ccatcggctg tgcagccatg gcgctgtcca ttacttcctg
    13741 caccaacttc ggttccccga acacctataa cctgaacgaa atcggcggag cccaggaaac
    13801 ctataccggc acggtcacca gcgtggaaaa cgtcaagatc caggccaata acgccaacac
    13861 cggcaccgga atcggcgccg tggcgggcgg gttgaccggc gccatgttcg ggggcggcaa
    13921 cgccaaattc gcgacggcgg cgggcggcgc cattctgggc ggcattgccg gcaaccagat
    13981 tgacaaggcc gtcaacaaca cgaccgggga acgcatcacc gtgcgtctgg accagaagag
    14041 gaacggcacc cgcaactaca cggtcgtcca ggtcgccagt aggaacaacc cgattcaggt
    14101 cggacagcgc gtgcgcgtga tcatcggcaa caacggttcc cgcgtgctgg cctattaatt
    14161 ggacatccac tactgaccaa gctatatacc ggcatgatct tcggcacctt acccggcggc
    14221 gcacctgtag aattgtttga actgtcttcc gacaagctga cggtacgcat cagcaactac
    14281 ggggggcgca tcatctccgt cgtcaaggac ggcatggaca tgatcgtagg gcccaagcac
    14341 tttgaagaca tgctgaaaga cacctgttat tgcggtgcga tttgcggccg tgtcgcgaat
    14401 cgcatcgcaa aaggcaggtt ctccattgac ggggattccc gcaccgtggc cgtcaacaac
    14461 ggccccaacc acctgcacgg cggcatccag gggttcgaca gcaaagtctg gcaggtgcag
    14521 gaagccgggc gcaatacgct ggtgctgacc ctgcactccg cagacggtga ggaaaattac
    14581 cccggcaacc tggaagtggt agccacctac accgtggtgg aagaaaccct tcatctccgc
    14641 ctggaagcgt atgcggacgc agccacaatc gtcaacctca ccaaccacgc ttactggaac
    14701 ctgtccggca aacccaccat tgattccatg accctggagg tacgggcctc cgcctatact
    14761 ccggtggacg cgaccaatat ccccgacggc cgtattcttc ccgtggaagg aacggacttt
    14821 gacctgcgga aagcagccct gctgggggaa cgcaactccg ccgcccaccc ggacaccgcc
    14881 gccggactgg accacaacta cgtgctggat tccgaaccgg gcgacaaaat cgccgccatc
    14941 cttcttgacc cggaatcccg gcaccgcctg attgtggcga cggatgctcc cggcctccag
    15001 gtatataccg gggaatacct gccccaggcg cgccagggaa tagcgctgga agcccagggc
    15061 ttccccaatg ccgtcaatac cccccacttc cccagcgtca ttctgcgtcc cggccaatcc
    15121 gccacgcgca gcattacctg gactgtcaga taggcggcat gcctgaatcc cgtttccgaa
    15181 caaaaaacgg cgcttcaaca acgcccgctg cgggctttcc tgtgcaggct cgcggcatca
    15241 ggaggccgcg taaagccctt ccgccgctac cttttggagc ccttccgcga tgcgttccag
    15301 ggaacggctt acaaaaacca gatccgcaac ggaaaccaga ttgtcctgat attgctcagg
    15361 caagcaggca aatgcctccg tcagggacgc cgcatgctcc gccaaatcct ccagcgcagg
    15421 gacaagctcc gccagagcct ccccgtccca ggaggaaaca gccttattca cagccctgaa
    15481 ccgctccgcc atttcccgat agacaggatc cgccaaggca gcctcccgga tgcgcgggcg
    15541 cttattcagc ctgttggccc ttttggcgat ggtcacggcc tcatcaccaa tccgttcata
    15601 catggaggac atgcgcaggg aagccagcac caccttgagg tcggaagcca ccggactgaa
    15661 aaaagccagc agatgcagcc cttccctgtc caccgtcatt tccagctcgt ccaattcttc
    15721 gtcatcagca ataacccggt tggcggaaga ctcattctgc tgcaaaaggc cggcgacggc
    15781 caaatcaagg gcgctctgcg ccttataggc catctggagc aaatgcgtat tcagggagga
    15841 aagggcggcg tcaaactccc tcagggtgtg cgcgtcgtga ttcatgattc ggaatattgg
    15901 cttgatgatt ggataaggtt ttgccggcat gataaaaaag atgctgcctg cgcaggctgt
    15961 tctttctggc ggcgctggca gccagccttc gtccagagcc gggaacatgg agaacacgcc
    16021 cggttccatt tttggaaata atctttgaaa aaagcataat cgtctctctg ttaactgaat
    16081 cttcccgtaa tataatcctc cgtttctttc agggagggat tggaaaaaat cttttccgta
    16141 gcatcaaatt ccaccagcct gcccaacatg aaaaaaccgg tcaggtccga aacgcgcgta
    16201 gcctgttgca tattatgggt gaccacaatg atggtaaact tgtccttgag acggtgcatc
    16261 agctcttcca ccctcaccgt cgcaatgggg tccagggcgg aacagggttc gtccatcagc
    16321 aggatgcgcg gcttcacggc aatggaacgg gcaatgcaca gccgctgctg ctggccgccg
    16381 gaaagaccat tggcggaagc atggagacgg tcctttacct catcccaaag agcggcatgc
    16441 cgcaggcttt cctccacagc ctcgtccaga atgccgcgtt tttttacccc cgccagcttc
    16501 aatccgtagg ctacattctc ataaatgctc atcgggaaag ggttggactt ctggaaaacc
    16561 atgcccacct cccggcgcag acgaatggta tccagggaag gagaagtcac gtccacccca
    16621 tcaatgcgga tggagcctcc cgtaacgcgc gcctccggaa tacggtcatt catccggttg
    16681 atacagcgca gcagcgtaga cttcccgcac ccggaagggc cgatgaacgc tgtcaccctg
    16741 ttcctgggga ctttcatggt gacgttgaac agggattgct tgctcccata acaaaaatcc
    16801 agggaatcca cctctacggc ggtctggtcg gctgctgctg gatgcggagt catgttcttc
    16861 taatggcgcg tcctgcgcct ctggacgcat ggtatcccaa aaacagggct ccctggctac
    16921 tccgggtttg tgacgatttc ttaaccagga ttctcttcct caccatgggg gcaggagggt
    16981 atcactaccc cgtcttccgc catgaacggc cccgccggac ggaaacggaa gacgcaacac
    17041 accaaaaaag acgccatgaa atttgtcgcc aaaatcctga ccatcgccgc cgccctcagc
    17101 tccctgagca tgggcgccaa ccaagtgacc gttgacagca gcatcaagcc gtatgcgccc
    17161 accagcggcg tttccggcaa cctgaacgcc gtcggctccg atacgctgaa caacctgatg
    17221 accctctggg cggaaggatt cagcaaaaaa tatcccagcg tcaaagtcgg cgttgaaggc
    17281 aaaggctctt ccactgcgcc gcccgccctc acggcaggca cggcccagct cgcccccatg
    17341 agccgccaga tgaagcggga ggaaatcgcc gcctttgaag ccaaatacgg ctacaagccg
    17401 acggaaatca aagtggccct ggacgccgtg gccttctttg tcaacaagaa caatcccatc
    17461 caggccctta ccctggcgca gatcgactcc attttctcct ccactttcaa gcgcggcggc
    17521 tccaacatca ccgactgggg cgatgcggga gtgccctcca tgaaaggaaa agccatctcc
    17581 atctacggca gaaacagcgc ctccggcacg aacgggttcg tgaaggaaat agccctgaaa
    17641 aaaggggatt ataaaaactc cgtgaaggaa cagcccggtt cctccgccgt cgtgcagggc
    17701 atcagttccg acgaacaggg aatcggctat tccggcatcg gatacgtcac ctccggcgta
    17761 aaaaccctct ccctggcgga aaaaagcgga aaggcggcgg tacagccttc ctacgataac
    17821 tgcatcaacg gaacataccc gctctcccgt tacctgctga tttacgtcaa caagaaaccg
    17881 ggagaacccc tggacaccct gaccagggaa ttcatcaaat tcatcgtatc caaagacggc
    17941 caggaaatcg tgaccaagga cggttattac ccgattcccg ccaaagtcag cgcagaagtg
    18001 ctcaagagca ttgaataaac actcccttcc cg
//
LOCUS       DAWEMX010000045        17793 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1324_length_17793_cov_13.515785, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000045 DAWEMX010000000
VERSION     DAWEMX010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_17793_cov_13.515785"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16329..16338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3601 a         4509 c         5445 g         4228 t
ORIGIN      
        1 atgccccgca ctctttttac tgtgcgtgca ctcagagagc aaatcccttt ttttcaataa
       61 tttatgttga agggtacggt ttgtttctca acatcaaacg gtcatggata cttcttcccg
      121 gaaaacgtct cttgttgtat tgttcaacca tcagtatgaa cgtaacattc cggtaatcag
      181 agagctttat tcaagacgct tttccgggtt gcttcaatta atgccctact acaagggaga
      241 tgcccctgac gtatgttccg tcttcgggaa ttctttccag ttctataatt atattttgca
      301 gtccagggaa agaatcaggc agttggaggg cgattatttt cttattatcg gagacgatct
      361 tcttttaaat cccaggtttg acgaattttc aacgccgtcc ctgctgggaa tccatgggga
      421 ggatacgtgt taccttgacg gttttgtgga cgtttctctg ccggtgtgtt accggggaac
      481 ggcggaagcc caccattttt ccataacccc tcccggtatt gatgcggaaa gcgtcaacag
      541 gaacgttccg tcctatgagg aggccaggcg gattttaaaa tccaggaacc tcatgcggca
      601 tgatgaattg tcgcgcgtac gcatgtttct gcccaaatgg agccccgggg ggattcatgc
      661 gaattggaaa gtgttgaagg gcagggtatg gcatctgctg aattactgga agcaccggat
      721 taaaaaatac cggtattcct atcctgtcgt tttcggatat tcggacattg tctgcattcc
      781 gaaagggaaa ttagacgatt tctgccgtat ccttgaagtg ttctccgcat ggaacatgtt
      841 tgtggagctg gccattccca cggcgttgca gcttcttccc ggaacaaagc tgtctacgct
      901 ggaggacacg cagtacaagt cgggaaacgt ctggtttccc caggatccgg aacactttga
      961 agccatgaat gccgtcattg acgggctcgt gtcctcttcc ggaggagacc tcggcaagct
     1021 gcttgcctcc tttccggagg aatacctgta tctccatccc gtgaaattgt cccgtttcgc
     1081 cagatagtgg cgtccataaa ttgttttttt atcccatgat gaaatatgct gtgatcggag
     1141 ccggggtttc cggattgtcc atggccggaa tgttgctgaa gaaagggcat gaggtagtcg
     1201 tttatgagag ggactcccgg cccggcggcc tgattaaatg cacggaagta caggggaacc
     1261 tgtatcatcg tgtgggaggc catgtgttca attcccggcg gcaggaagtg ctggactggt
     1321 tttggtcccg gtttgacagg gagcgcgatt ttgtttccgc ccggagacgg gctgttattt
     1381 ccctggaggg cggggctgtg gtggattatc ccattgagaa ccacctcgac cagtttcccg
     1441 aggccgtccg ttcctccatc gtccatgagc tgctggaact ttacaggaat cctcccgcgg
     1501 agccccgctc cctgggtgag ttttttctga accgtttcgg aaaaaccctg aacagccttt
     1561 attttacgcc gtacaataac aaagtgtgga ggcaggatat cagccaaatt gccatggatt
     1621 ggctggaaga caagcttccc atgccgagcg tggcggaaat cctgttgaac aatattgggc
     1681 acatcaatga aagcgccatg gtgcacagtt ctttctttta tgcgaaaaac ggcggttccc
     1741 agtttctggc ggatacgctg gctcgcggcc tcaaggtcag gtatcggcag gaagctgtaa
     1801 atatcctccc gaaggacggg aaatggctcg tacagggaga attgtttgac agagttgtct
     1861 ttacggggaa tgtcaggcag cttggggatt gttttccctg catggatgaa ttgcggccgt
     1921 ttttcccccg gatttcagaa ttgcgctctc acggaaccac ttccgtgctt tgccggattt
     1981 cccccaatga ttacagctgg atttacatgc cctctccatc ccaccgctct caccggatca
     2041 tttgcacggg gaatttttcc agaaataata ataacgggga catcaccacc gcgactattg
     2101 aattttccga gcaaatgatg gaaggggaaa tcaggcgcca gctggagctt attccctttt
     2161 cccctgtgta tctggcccat cactgggaag aatataccta tcccgttcag gatgtttcca
     2221 gccgaaccct catacgggaa ctaaaggagt gcctggaacc gaaaggcatt tatttattag
     2281 gccgttttgc ggagtgggaa tactacaata tggatgcggc catgggggcg gcccttgatt
     2341 tggataaaag gctggctgcg gagcagatga cacggggata agtcaaattc tggacattgt
     2401 ggtgtatttg aacgggggat tgtaaatccg cctgttttta aaaaagtttg gatatagtta
     2461 taaaaagata actataaata acttattgtg ttgaatggtt ttttggtaat taaaaaaatc
     2521 ctgtttcaat ccgtggttcc atgcacggag atgattctcc gttaaagttt tccattgaca
     2581 gcgaatttac aactcgttat aggtacggac gcagaagatg tgttcccgtt tttagggaca
     2641 tggtttccac cggaatctgg gagtatggct atccgttatt gttctttcgc gacccgcggc
     2701 tatcatttgc ggcaatggat gcagatgatg agcctgaggc tggcggattc ctcagtggag
     2761 ttgtcctgtt ggaatcttaa tcgcctggaa agggaaggaa tcagggaggc tattccatgg
     2821 tttgacctga atttacaggg tgtcgggttt tgggtatgga aacccttcat cattctcaag
     2881 gagctggaac atttggagga aggggattac ctgctttata cggatgcggg gcgcccgccg
     2941 gggaggcttt tccatcattc cttggagccg tttgtccaat ggctccggga gaaggggcag
     3001 gatatgatgc cgggcgtgaa tatttcctat tatacgggag gccttacgcg gtggtgcaag
     3061 cagagcgttt ttgatgaatt gaagctggat agcgggtctt ttgaacaggc tcccctgatt
     3121 cagggctcct tccttttctt gaaaaaatcg gaacaaagcc tccgaattct ccgtgaatgg
     3181 atggatttgt gccgtcagtt caaattggtg tcttctgcca ctacggaaga agagagccgt
     3241 tgccgcgatc attttgtagc tcatacgcac gatcagacgc tgctgtctct cgtggcggcg
     3301 caacatcatc tggactgcct gtattccggg aagggcgtca ttcatctgga aggccgccgg
     3361 gcgtttgagc ggcatcccgg ttttgacgac aaagacccgg actggttttt ggaagagctt
     3421 ggcgcaaaga aagcggagca tcccttaatg cccgtgcttt gctgcatgca acaggcgttt
     3481 ttcctgtatg aaaaagccat gaataaattg atgaagctgg cgggcttgag acccctccgc
     3541 gggttttacg gttgatggac ggttgtgtgc ggaaggggag tttgcctgtt tcggcctccg
     3601 tgacggatct ctccggcctg ctatggacga aaataaaaaa gcgccggaaa aaccggcgct
     3661 tggaaaggta gtttcctgtg tggcttttac caggaagcgt cgaatttggg gataggcgtt
     3721 cctttgcctc cggggctgtt ttccggaact ttataggttt tgcgcagttc gtggtaacgc
     3781 ttcttgagct gttccaccgt agtcttgtag gcaggatcgt caatgacgtt tttcatttgc
     3841 atgggatcct ttttcatgtc aaagagcatc cattcgtcgc tggtccagat gtaggaaagc
     3901 gtgtagcggt ccgtccggat gccgtcgtgg cgcggggcgt tgtgttcgcc gggattttcg
     3961 taaaaacagt agtaaatggc atccctccag ttgtcgggag ttttgcccgt gaaagcggtg
     4021 ggaagcaggg atacgccctg gaaggtattc atgttttccg gggtgtccgc ccctgccgcg
     4081 gaaacgatgg tgggagcgta gtcgatattc tgcaccatgg tgttgttgcg gatgcccgcg
     4141 ggaattttgc cgggccatct catgatgagg ggcatgcgga gggattcttc aaaaatccag
     4201 cgcttgtcgt acatgccgtg ttctcccatg tagaacccct ggtctccgca gtagatgacc
     4261 agcgtgtctt tcgcaatccc ctctttgtcc agatattcca taaggcggcc gatgctgtcg
     4321 tccacggaca gaaggcatcc cagataatcc tccatgtagg catgccactt ccattccgca
     4381 aacgctttcg ggtccttcag ttttccggat ttcatgccgt ccaccaggga tttggtgcgc
     4441 ttggcgtaat agtccgtcca ggttttcttt tcttccggag tcatgcggtt caactcgccc
     4501 aggtcccagc cgtaaccggg ggaaacgatg cttttgcgca tttcttccgg aacctggtcc
     4561 ttaagcactt tgaggtcgga ataaatcgcc atgtgattgg cgactgtctg ctggtttttc
     4621 ttcaggaatt ccggacggtt ggcatagtcg tcatggaagt tggcgggcgg cgtcatgctg
     4681 gaagtgtcca ccttgcccag gtggcgcaga gcagggcacc aggcgcggtg gggagccttg
     4741 tggcccacaa cgagcaggaa aggcttattc ttgtcccggt tttccagcca ctggatggat
     4801 ttgtccgtga ccacgtccgt ggcatatccg ggaaaacgct ttgtctggcg tttgccgtcc
     4861 ggcttgaggc tgataaagtc cggattgtag taactgccct ggccggggaa gatttcccac
     4921 gtgtcgaacc cggtggggtc cgattccaga tgccatttgc cgaaaaggcc cgtctggtat
     4981 ccggccttct gcagcatttt cgggtaagtg ggctgggagc cgtccagcgg tctttgtccg
     5041 ttgaagacaa aaccgttcat gtgggaatgc ctgccggtca ggatgcaggc gcgggagggg
     5101 ccgcacaggg agttggcgca gtagctgcgg tcaaaaacca tgccttggcg tcccagtttg
     5161 ttgaaattgg gcagagccac gggggaatca ttattgccgg tgcccagcgt ctggtaggcg
     5221 tggtcgtccg taatgataaa gaggatattc ggttttttat tttcctcagc cttggtttga
     5281 gctgagaggg gagctgccgc cagcagggcc agagttccgg cgatgatggt tttcaaaggt
     5341 ctcatattgc ctggaggata taggatgacg gcgccgtgtc aatgtcatag gattcctttt
     5401 gccgggattc tgcgttatgg tattcttgcc ccatgctttc ctctgctctg gaagacacgc
     5461 tgcgtttgtt cggcaaggag cggttccgtc ccatgcagag ggagcttgtg gagtgcgcgc
     5521 tggagggccg ttcctgcatc ggtattttgc ctacgggttc cggcaagagc ctttgctatc
     5581 agattccggc ggttctggga aatgggctga cggttgtggt ttctccgttg attgccctga
     5641 tgcgcgacca ggtggcaggg ctggaaaaac tgggcgtggc cgccgccagg tatgattcga
     5701 ccctggcgga agaagataag gtcgccctga tggacagatt gaagtccgga ggcgtccgcc
     5761 ttctgtttgc cgctccggag tctctggaat ccccgtggat tcagcaggtc atggagtttg
     5821 ttccccccgg cttgttcgtg gtggatgagg ctcattgcct ttcggaatgg gggcacagtt
     5881 tccgtccgga ttatctgggg ctgcccgttt ttttcagaaa gcacgggttc cgcagcgtca
     5941 tggcgttgac ggccacggct acggagcagg tgtgccgcga tctggccggc ctgtttggcg
     6001 tgagcgatga atgtattttc cgggctgcgc cgtaccgtgc gaatatcttc cgccaggtgg
     6061 aaacgttgaa ggatcaggat aaaaccgcgc ggctggttga attgttgaaa gagggaggcc
     6121 gccgtcctgc cgtggtgtac acgcggaccc gcaaagatgc ggaaaacctt tcctacgaat
     6181 tgggaaaagc cggtttctcc gtcaagtcct accatgcggg catgcccccg gagacgaggg
     6241 ggctggttca ggatgagttt ctggcaggta cggcggacgt gctggtggcg acgattgcct
     6301 tcggcatggg gattgacaag ccggacgtgc gttccgtggt gcattaccat ccccccgcca
     6361 gtctggaggc gtatgttcag gaatccggcc gtgcgggacg cgacggcctt ccttccttca
     6421 gcctggtgat gttgtccgcc cgcgacagcg tggccgtagt caaccggctg catgcggcgg
     6481 agccggaccg gcacggcatg aaagggctgg tatccctgtt gtcccgcagg ggggagcaca
     6541 ttgtttccct gtatgaggct tcctccgttt atgatttgcc ggatgtggcg gtggaccgga
     6601 tgctgtttga cctgaaacgt tccggttccg tacgggagaa ggggacgggg ttcaagtatt
     6661 acagggtgcg ccctctgttc cggatggagg aaattctgtg cggccgcggc ggggaggaat
     6721 gcgcccggct gcaatggatg gatatgcgcc ggcagggaga ggtggaggat ctggctgtgg
     6781 aatggggaat ttcatgggaa gaagccgccg cctggcttgg tgacctggct ctttccgggg
     6841 aatggaaagt ggagctgcgc caggcagccc ttcatctgta ttcggaagga tttgacgcgg
     6901 aagaggctgc ggaggaattt gagcagtatt tttccagatc ccggctgaac gggctggaac
     6961 gctggaaaac ctgcgtttct acgctgacgt cttccgcctg cctgaataga agcctggatg
     7021 cctatttcgg tttccgggac ctgtccgggc cttgcgggca ttgtcctgcg tgctgcggga
     7081 tgttgcctgc ggccatggaa gaagaggaac ttcccccgtt accggaagag ctgcgctccg
     7141 ccgtcatgga gctggcgggg cagaggaagc ccgcgctggc ccgcccttcc cagttggcgc
     7201 gctttttgct gggactggct tctcccgcgg ccatgcgcgc ccggttgtgg agccatcctc
     7261 tctacggggc gctggcggac aggaaatggg aggatgtgtg gattgaggcg catgccctgt
     7321 cgggtagtta gaggccggtt tttccgcctt gggtggcttc ataggcggca ataatgcgtt
     7381 tgcaggaatt ttccgcatgc agcagggaac accaatagcg ggatatttcc tccgggttgc
     7441 gttttccgga gtcccaggcg cggatttctt ccaaaagtgc cccggagagg ctttccggag
     7501 aatcgttcgg gctgagtgtg cctgaatcat gggaaacgta ccattgcagg cagttcagct
     7561 ggggtgtgag gcgaggccct acgacagagg ccccggcgca cagcgcttct gcggaagcaa
     7621 ggtgcgttcc ttcggacaag gaggtgcaga gactgattct ggcctgggat tgtttctttt
     7681 gcagttcgat attgggaata tagccagcca gatgaatccg ggtgcgaaga ggggccggaa
     7741 gattgccgtg ccattcccgg agaaagtcag gaatattgcc gtaaatttcc gcttcccagt
     7801 gcggggcgtg gggaagcgcc tgttccagcg tggatatcag caggcagggg cgtttataga
     7861 gaagatcgtc ccagcggcct acggccataa tgatgttgcg cttctctcct gcaaagcggc
     7921 aggtggaaga aatggggttg ggaaccagag ccagtttccg gacgatggat tttccataga
     7981 gccatttcct atctgcaacg gattggaggg ctgccggaat ggggaaggcg accatgtccc
     8041 ctgagtccaa atgttttctg cgccccctgc tggcgaggtt ccatgtgtgc tgtttcagaa
     8101 ggcgcagcag gaaaaaagcg gaacctttga ttttcccgtg aatgaaaacg tctttggtgt
     8161 acatgttttt cgcgatggga agccagtatt gccacggaaa aaacagcccg gatgagtcca
     8221 tgtacactac cagaaagatt cccgcgtcat ggatggctct ggcgatggga aggtatttgc
     8281 ccactcccca ggcgtacagg atcactccgt caatacccag ggatttccac caggctgcat
     8341 ctttcaggtt ggcgtattcc gtgcggatga ggtcgggatc atctccttcc tgggcaggtg
     8401 tgggcatgat ggccctgctt tccacccccc cgattctctg aaaggccttg cagaaagcgc
     8461 ctgaatcgcg ggaaaagaaa gtatggtctc ccttgaagga caccggcgtg catgtgtacc
     8521 atttcatgat gttggtgggg tggaagatga atggaaaacg cggagctcgc caagaagggc
     8581 ttccatggac cggagccatg cggcggggga gtatgtggag gacaaaagtt gttgcccgtg
     8641 cgcgcccatg gccggccagc gttcttcttg cttcagggcg gtgtgcagcg tctccgccag
     8701 ggattccacg gaaggttcct gtgcaatcca gccattcctg ccgtgggtga tgagctccgg
     8761 caggccgccg ttcggggtaa cgatgacggg cgtgccgtgg agccatgatt ccaggacaac
     8821 cataccgaag gtttcctccc agagggaggg cgctacggag aggccgcagg cagcgcggac
     8881 ggctccttgt tcctcatggg gaatgaaccc ggtcaggcgg atggattcaa gatggcggga
     8941 agagatataa cgctccagtt ctccgcgcag ggggccgtct cccatcaggt agagaatgcg
     9001 gccggggggg gataggagtt cccacgcctg aaccagttgc aggactcctt tttcctgcgt
     9061 caggcgtccg gcgtaaagga cgtcccggcg gggcagctcg gcggcagggg tgggattttg
     9121 ccggatgaaa tgtggaataa ccctgatttt atcctccggt attccggttt ggatgagaag
     9181 ttcctgctgg cgccggctga tggcaatgta ggaggaaaag cggctccagg ctcccatgcg
     9241 gcgcgtttta tacaggacgg cggcggccag ggaggagtac gcggcgtttt tcctccaaca
     9301 ggcgtgcatg atgccgggaa aatagttgcc gccttggcag gaaaagcagg gcgctccgtc
     9361 ccggtaaaat actccgttga ggcagccgga gcggtaattg tgcatgtagt ggatgacggg
     9421 agcgggttga tgcagggcca gttcatagac gcagggggac atggccggga acgtattgtg
     9481 gatgatccat gcgtcatagc ggttttcacg caggcatttg cgcagttttt gctccacatc
     9541 cctgttgtgg agcaaataac ccattttcgt cagggcgccg tgggggctat ggaacagctc
     9601 ctccgttgaa tagacaaagg gtgtgacatc cgctatggcg cccaaagcct ccgtaatggc
     9661 atggaagcag atttcttccc ccccaacctc gccgtaacgg ctgaaaatat gtagaatgcg
     9721 tgggcgttgc atggctgcgg cggcacaggg ggttatccct ggagttccct tctgagtttc
     9781 gcgggatcct tcaatagctc ccgctgaatg gcttcctgct gctggcgcag acggtcttcc
     9841 cgtaactgaa gggatttttt cccggctccc agcccggcat tgacaaaggg attgttgtcc
     9901 ccgtatttgg accacgggcc gcggggaatg cggtaaaatt ccacaaagcg ccagtggaca
     9961 atggctgttc ctcccgtaat gcccagatag tcgcgtacgg caggggagat gtcaatgccc
    10021 gcccctttgt tctgggtatt gacgggaggc ttgtctccga agacgtaggg ccagtcttcc
    10081 gtggtgaagg ggccgcagtc ttcccattgg gcaaaacagg aacgcttgtt gtagacgatt
    10141 tgcacccatc tgcccttgca gacggattgc ccctttcccg catattcgcg gcggaaccac
    10201 gggatgaccc gtgacgcctc cgccttatgc tcccctctgg aaacgtcatt ataagggagc
    10261 gccacgtaaa aggggttttg ccgggggatg aatgttttcg ggcggaagtc cctcgtgcgg
    10321 caaacgggat ccgggttgtc gaagccgccg aagttttccc gccatgcgga atcccacgag
    10381 cttttgtggt tggctaccgg attgcgttcc gtgggttctt cccccaccca gaagaccgtg
    10441 gcggtaatgt tgagacgcca gggataggtg ccggggcttt gcctggacgt cctgggaagg
    10501 atgcggggag cgggccgctt tcctccctct gcagcaacag gcgatttgac gatgacccgg
    10561 ggcagggggt cccgttgcgt tggggtgacg atgacggggc ctttgggccg tccggaactg
    10621 ttcgccaggg cgcgggtggc gctccggcct gtctggatgt ccgccctggc ggtttctgcg
    10681 gaaaccagaa ccggcagcgt gagcaggcat gcggacagga gggggctccc gaagaacccc
    10741 ttccgtaatg cctgttgacg ctgccggtag tttaccatcg gtgcagttgg gtgacgaagc
    10801 ggtggtccag cttgccggga taatccgtat tgaaatgaag gccgcggctt tcattgcggc
    10861 gcatggcgca gtccacgatc agggaggcca ccgccaccag gttgcgcaat tccagaatgt
    10921 ccggcgtaat gcggtatccc cagtagaagt cctttacctc ccggtgcagc atccggagcc
    10981 gcgtggctgc gcggtccagc cttttattgg tccgcacaat ggagacgtaa tcccacatca
    11041 ggcggcggat ttcatcccaa ttatgataga tgacggaaag ttcgtcaggc agggctgtat
    11101 ctccgtggtg ccaggctggg atgtcgtaag gagtggggga atcctttctc aggggatgca
    11161 gggagagcat ggagttcagc gccctcttgg cgaccaccac gcattccaga agggagttgg
    11221 aagccagccg gttggcgccg tgaagaccgg tgcagccggt ttcccctgcg gcgtaaaggc
    11281 cgtccaggct ggtctggccg ttgacgtccg tcagcacgcc gccgcactgg aaatgcgctg
    11341 cgggaacaac gggaatgggt tccctggcgg gattaacgcc gtagcggttg catgtctcgt
    11401 aaatcagggg aaaacgttcc tggacgaacc cttccggctt gtgggagata tccagataaa
    11461 cgcacatgct gcccgtcctc ttcatttccg aatctacggc gcgggcggtg atgtcgcgcg
    11521 gggccaggga tttgcggggg tcgtacttgt gcatgaattc cttcccgtcc gcgcctatca
    11581 gcacgccgcc ttccccgcgc acggcttcgg agatgaggaa ggagcgcgct tccgcgcctt
    11641 ccgcgcgggt gttgaagaaa caggtgggat ggaactgcac gaattccatg ttggcgatgg
    11701 tggcccccgc ccgccaggcc atggctacgc cgtcccccgt ggcggaatca ttattggtag
    11761 tgtacagata tacgcgcccg cagccgccgg tggcgagcag cacgcggtct gaccggaaga
    11821 tttccacttc cccggagact cggtccagca cgtaggctcc cagaacgcgg tcttccgtga
    11881 cgaggcccag cttggtggtc gtaatcaggt caatggcgaa atggtcttcc agcagttcaa
    11941 tgttggggtg aagcttggcc gcctccagaa gtttggaggt aatttccagt ccggttgcgt
    12001 ctttggcgtg gaggatgcgc cgtttggtgt ggccgccctc cctggccagt tcataatcct
    12061 ctccctggtt gccggggtca aagttcactc cccattccag cagttcccgg atagcgtccg
    12121 ggccttcctc cacgatagtg cgcacggcct gttcgttgca gaggcccgct ccggcgtcca
    12181 gcgtatccgc aacatggtct tcaaaggaat cgttgcggtc ccatacggcg gcgatgccgc
    12241 cctgcgcctt ggcggaactg caatccagaa atttgccttt tgtaatgact gcaaccttgc
    12301 cgtgttccgc cgctctcaag gcgaaactca atccggcaac acctgcccca atgactaaaa
    12361 agtcgctcgt cttcatcttg aatggtacgg aattgtacca tgacgcgggc tgccaagtca
    12421 cgcccaatga tggggcggca gggcatattt gtctggaaaa gaagtgtgaa acacgctaat
    12481 tcatgggcat gagaccccaa gggaacgaac aggaagcgcg ccggaacaag catgtgcgca
    12541 gcgcgccgtc caaaattgcc gtgaaggcgc tgggagagga ggtgctggac gggattctgg
    12601 acaaatcctc caaccctctg ctgcttattc tggactgcgt gcaggatccc cacaacctgg
    12661 gagccatcct gcggacggcg gacggcgcgg gagtggatgc cgtcatcgcc ccccgggaca
    12721 aatccgtggg cattacggag acggtgctgc gcgtttccgt cggcgctgcg gaaaaggtgc
    12781 cttttattca ggtgaccaat ctggcgcgca ccatgaagca gctccagagc cgcggcatct
    12841 ggatattcgg cacgtcggac aaaggagacc gcggcctgta tgaaacggac ttcacggggc
    12901 cggccgcact ggtgatgggg gcggaagggg aagggatgag aagattgacg gaagagaact
    12961 gcgatttcct gctgaggatt cccatgaagg gaagcattcc gtgcctgaac gtatctgtgg
    13021 ctacgggcgt atgcctgtat gaaatagtgc ggcagcgttc ctgattccat gactgttttt
    13081 ctccttccct cgtctttatg attcaggaac ttgatcttgc tccgggggaa cgcgccaggt
    13141 ttatttcaga catccatttc ggccatgcca aggcgctggc ccgggagccg gaagagctgg
    13201 ggtttcttct ggaggggtgc tcccatctgg tagtgtgcgg ggacctcagt gaaacccgtg
    13261 aaagcccctg ccgggaggaa ggattggaaa aacgggcccg tttcctgcgg atgtgccggg
    13321 atgccggggt gcagcccgtt ctgctggccg gcaaccatga tccggacgag aaagccggcc
    13381 tgctgaaatt gcagggcggc cgcatctgcg ctttgcacgg ccatgcgctg ttcagggagg
    13441 tggctccgtg gggatgggaa tatttgaaaa acaagcaggt ttcccgcgaa ctgatcgcag
    13501 cgttcccgga ggcggaggca gatttgctgc ggcggctgga gctggcccgt gccatgagtg
    13561 tgctggtgcc tcctgtttat acgcgttccg gaacacatca aaataaactg gtgcgttttt
    13621 tagctcattc tgcctggccg ccggaacggc ccgtgcagat tcttctggca tggctgacga
    13681 tgatgcggcg catggggaaa tttgcagacc gtttttttcc ggaagcggaa gtcgtcattt
    13741 ttggccatct tcaccgacgg gcggtgagcg ggaaaaaggg gcggcggctg tacgtcaacc
    13801 tgggcgcctg tttccgccat gccagatgct gggcggcgga cgtaacgccg gaaagaggag
    13861 tctccatccg cagttatacg cccggaggat atgatggccc ggaggcaatt ctccgctgat
    13921 tgatgcccac ggaaaaaaaa gtgaggggaa cgcgtccgtg tttaggcgat aatgtgttag
    13981 agcaccggat gactagtcca tacagtttgt ggacgcgttc cccatagaat ttggccgcgc
    14041 ctgttttccc tggtagccgg cgggacagtt tttcccggag cttttcaggg cgcagccccg
    14101 gcagcagcag gggcgcgagg aggaaaaaaa cctggagaag aagggctcca ttctggcggg
    14161 tgcgccgccg gagcactttc tttcaccgtt tctttccgaa ttggagctga ccatgaagca
    14221 aagttagtgt aatctaactc atattgcaag aaaaaagttt gtctcgccta atatttttca
    14281 agaagaggtt gacttggtca gagccttggc tattgttttt tgttagttaa acctaataag
    14341 catcaatcat gatacatgaa aaattttcca ctccggagtt gagcaacagc ctggaggatt
    14401 atattgaggc cgtccgcaac attgagttgg aaaagggatg cgccaccgta ggggaaattg
    14461 cggaatccct gaacgtgaaa aaaccttccg cctcactggc catgaagcag ttgaaggaac
    14521 gcggcctggt ggaatacacg cagtattctc ccattgtcct gaccagggaa ggccgttatt
    14581 acgcggaccg ggtcatttcc tgccatacca tggtgaagga gttcctcatt acggtgctga
    14641 agatggagga aaaacgcgcc gatgaagtgg cctgttgcat tgaacatatc atgactctgg
    14701 aggaaatctc ccgtttccag cttctgacag atcgttgcag ggaaagagga gaataaaata
    14761 ctcccacatg attttccggg gggacggttc atgagggaat ggctcatccc tctctttttt
    14821 acttcggaac ccggaccgag ggaaggcatc ggcgtgtgct gcgcgcgctg acgtacactg
    14881 tggaagcgtt atggaggaac gcggaaaggg ccggggtaat cagcccgaac atgcccagca
    14941 ggatcagaga tgagttgaac ccgacgataa agcggtagtt acgttctatt ttttcaagga
    15001 cggcacagct caattccctt aaaacaacca gttcagccag agagctggac aacagggaaa
    15061 tatccgccac ttcacgggcg atatcggaag aatccttcat ggagacggaa acatctgcac
    15121 aggaaagagc gggggaatcg ttgatgccgt ctcccaccat gcacactgtt ttgccggatt
    15181 tcttcaattg ctggataaag cgggccttat cttcaggcag aacctgggaa cggtattccg
    15241 tgatgcccaa ctggcggctg atggcgcggg ctgcggattc gctgtccccc gtgagcatga
    15301 tgatggagga aatgccctgc cgtttcagcc gggcgatcgt ctctgcggct tccggcctgg
    15361 gcgggtcgct gacgccgatc atgccgattg ctctgcggcc tatggcgaga aagatgttgg
    15421 attttcctct ggcgtgattg cgtatggtca gcctttgctc ttccgtgagg gggatgcgtt
    15481 catcttcaaa gagaaaatgg tagctgccca caagaacctt ttttccatga aggcgggagg
    15541 aaatgccgtg ggccacgatg tattccacct ccgcgtgttc ttcttcatgg tgcagaccct
    15601 cctccagcgc tttgcgcaca acggcccgcg ccacgctgtg ggggaagtgt tcttccaggc
    15661 atgctgccgt tttcaggata tattccctgc tgcagtcgct caaagggatg atctgcgtta
    15721 cctgagggca ggcggaagtc agcgtccccg tcttgtcaaa aacgatggtg tccgttttgg
    15781 cgaacagctc catgaatttc ccgcccttga tcatgatctt gcgcgctgcg gcctctttca
    15841 tggcggagat gacggagatg ggggtggcga gcttgatggc gcaggaataa tccaccatca
    15901 gcacggagag agccttggag atattcctgg tgaacagaaa cagcgcccct gccgtgagca
    15961 gggtataggg aacgatagaa tccgccagct tttccgcccg gctttggatg cgcgctttca
    16021 gttcctccga atggtcgatc agggcgatga tattgttaat gcgggactgg tctcctgcgg
    16081 cgtccacccg gatgacgagg gaacccgttt ccagcgtggt tccggcaaag acgacggacc
    16141 cttcccgttt ggaagccgct tccgattccc ccgtcatggc ggcctggttg acttccgctt
    16201 ctccggacag cacggtaccg tccacaggga tgacgttccc cgcgtccacg cggattttct
    16261 cccccgggcg gatttgctcc atgggaacct ggcgcatgcc tccctcttcc acccgccaga
    16321 cgcggtctnn nnnnnnnnat gctcaggctg gccgcaagcg ccgcccgggt ctttttccga
    16381 gtgtaatttt ccagcaggtc tgaaatggaa agaaggaaca tgatggaatt ggccgttccg
    16441 taagccttcg tgccgataga aaccccgatg gagacggcgt ccagaacggc tacattgagc
    16501 cgtccgcttc ccaggccggc cattccgcgc cgtaaatagg aaccgtaatt ccagaatgct
    16561 ttggctattc tgagcggggg cggcagaaac accttgcaaa acaggaaaga ggaagctttc
    16621 cccgctatgc ggagaaaaaa ggaccgggcc agtttcctgg cctccgcgcg ctcttccggt
    16681 tcgtggctgc ggagtgtttc cggcctgagc ctgtccagcg tgcggaaaat ggcctggggg
    16741 ctggttcctt catacaggat gaacacacct ccgttgcagg gggtggcctt gacggagtat
    16801 acgcctttct gtttcaggag cctgcattcg atgccgtaag cctgatcatg attgaatgcg
    16861 cagcggccgc tgcgtacacg gagcctgccg ggcgtatgat ggataacctg gtaatgcatg
    16921 gataaggaaa aagaaaaggg ggaaacgccc ttctccgtgc gtttcccccg ttgggggcgg
    16981 tccattccgc tggcggagaa ggaaacgggc accccgctgt ttggtgatgg acaatgagat
    17041 tgctactgtt ccggcctgct gtttttcgcg gcggcctcat ggcacaggtc ctgggcttct
    17101 tcccggatgt ttgccagttg ttccatggcc ttctgctgga tgagcatggc cttggaaagg
    17161 ccctggacgg ccagtttcct ggcaacggga ctttttacta ctttcgctcc gatggcggcg
    17221 gcgatcattc ctccgacgaa gccccagagg cctttgttgt tcatgtaact catggtaatg
    17281 ttttgattgg gttcatccgg ccttggcggc atgcagccgc ttcttttccg gaaggattgg
    17341 attatatcca atccagcctg cattttttta gcgtgccccc gtattctggt caagatgaat
    17401 tagctgaaac taatttttta gcagacatga aaatattttt gcgatatttt taatacgttg
    17461 aaaacaaata aaatataatg aaccaacata atgttgtact gagcagggca agggcattgg
    17521 gaagaaaaag tgttgactga atagttagtt caatctaact ttgccgcgtt gcatgaactt
    17581 gacccataca ggacttcacc gtttcttccg ccgggtctgc cggtacagga aactggccgg
    17641 gctgctcatc cggcgtccca gataccgtct gctctgcgga gactgtgggt tctgtatggg
    17701 ggcatgcggc agagctgtga aagagcaagc ccgggagaac ggaagcggag ctctttccct
    17761 gtaaaaggaa agcgcccctc tccacctctg gat
//
LOCUS       DAWEMX010000046        16736 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1436_length_16736_cov_14.761645, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000046 DAWEMX010000000
VERSION     DAWEMX010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 16736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16736
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1436_length_16736_cov_14.761645"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11462..11471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3859 a         4992 c         4393 g         3482 t
ORIGIN      
        1 ggttgccatt gattctggta tgatattccg gccgccaggc cccttccccg cgcgtgtccc
       61 ccagctgcgt caccaggcaa tggatatacg cggcctcccc gtgaagcaaa tcgccgatga
      121 cgccctgccg gaccatgttc aggaacatga gttcatcccg cccatagttg acgttttcca
      181 tcatcatgca atgtttcctg gtggcctcgc tggtatccac caggctccag agctcctcca
      241 gagagacggc cagaggaact tccacaaagg cgtgagcgcc gtgcttcatg ctgtcgcagg
      301 cgattctggc atgcgaactc caatccgtac tgatgatgac ggcatccggc ttcagctcct
      361 tcagcatggt caggtattcc atatcgccgc cggaatagcc caaagggctt ttccccattc
      421 tggagagcac gcgatccacg ccgcgtttcg tcaactcttc cttcaaatca catatgccca
      481 cgatttccac tccatccatc acgcacattt gcgccaggtg ggagaacccg cgccctccca
      541 cgccgatgaa ggccagcctg atgcgaccca gccggggagc cttgaaccct cccatgtatg
      601 tggaaccgga aggcctcgga gccgccggag aggcggattt ccagagatgg tctccggcag
      661 ggatttccac agccttccca gggagggcat ttccggcggc agccgctccg gccgcaagcc
      721 ccaaagattt gagaaattgt ctgcgtgatg agaaaataga catggcttga aagtatggct
      781 tcaaggaaaa ctacgccccc tccggagtga atttatcatc attctcccta ttgaggggaa
      841 aagctccgga tggctgattt tagagtttgc aaaatccggc ggaactccca ctatgagatt
      901 tacttctgac tctacccttt ttttcattga tgcgttacct acttctcatc ctgttgctcg
      961 ccgggggata ctacggctgg gattattatc aaaacggcct cgcacccagg catgaagccc
     1021 tcaaagccat ggagcaggaa ccctgcccgg agctggatgc ggcccaagcc cgttttgatg
     1081 aagccgcgga acttcacgaa cagcaggcgg cgcgtctcca gaaagctatt caacggaagg
     1141 aagaagccgt ccgcaaatac tgggatgccc gaataaagca agccggccag gaaaaagacg
     1201 ggaaaaagac gccctctccc gtaagcggaa agcccaatgc ccgaagcgcg gaagcccgaa
     1261 tagcccatct gctggaacaa tatgaccggc gcgttgcgga agtggaagaa ctcaaaagca
     1321 ggagggacgc caccagacaa caattggaca gcgcccgaaa ccggcttcag gaacaaatac
     1381 gccaggtgga aacccgtctg gacatcaacc gcatccagct cgcggaggcg tccaacacga
     1441 aaaacaggaa ttttaaagtg acggagaaca ggacggatct gctcaaactt caggccagtc
     1501 ttccgcagga actggagcgc attaccgacc agggggaagc cgctctgcgc gaccagacgg
     1561 aagcttacga aaaggccaaa agggagttgg acctgttcca aaacaatgtg gacaggcaga
     1621 tcgccagtct tcgggacacc atgaactccc ctctggcgga agattatgat gagacggaaa
     1681 tactttcgga agaccccgaa ttccgcacga tgattgctcc ttacgaccag gccgtcagcc
     1741 atgaggaaac gattgtcatg cgaacggagg cggatgtgga ggaacaggcc aaagccctgc
     1801 acaggctgca acgcgtcaga gacgcgaaac tcgaagagga gcgcagccgg ctcagacagg
     1861 aagaagaaat cttcttcacg gcggcagtcg ttatcggcgt aatattgctg atctccattc
     1921 tgctctcttt cgccagaagg agatgatgtc tttgaaggtt gacactacac tgcaaaccta
     1981 gtttactggg tgagccgccg ggaaccccgt ggaggcacga ctggcaaacc ccgccaggac
     2041 cgagaggtag caacggtagc ttgttcgtgt ttgccgcgtc cgttcccggc ggcattttac
     2101 agtccccacg ccataccgga gttgaacggg agttttcccg tggtaaaata ccgcccatga
     2161 atgtatccgc cgcccttgac tggctctttt ccacccaatt tttcgggatc aagctggggc
     2221 ttgataatac caggaagctg ctggcggcag ccggggcgga ccggataaac gccaccgtgg
     2281 tccacgtggc cggcaccaat gggaaaggct ccacctgcgc catgattgag gccctggcca
     2341 gggcgcaggg ttatgtcacg ggcctgttca cttcccccca tctggtagat ttttctgaac
     2401 gcatccgcgt caatggggac atgatcactc cggatgccct ggcagaagaa atttccttcc
     2461 tgaaacagct ggcggaaggc tgggaacagc cgcccacctt tttcgagctg gctctggccg
     2521 tcgccctgcg ccacttccgg aaaaacagcg tgaacttcat cattctggaa acagggctgg
     2581 gcggaaggct ggacgccact aacgccgtgc ccaaggatat cgccgtactg gcccccatcg
     2641 gcctggacca ccagcagtat ctgggagata ccctggaaga gatcgctgcg gaaaaagcgg
     2701 ccatcatcgc ccccggaaaa ccgtccgtaa ctgccgtgca gcacccgggt gtcatggccg
     2761 tcatcgaacg gacggcgcaa aaccgccgtt ctcccctcac gattgcccgg gcggaccgga
     2821 aaacgcccat cccatccctg ccaggagccc atcagcggga aaacgcagcc ctggcgctgg
     2881 aaaccatgag gcggctccat tccctcccct ccccatccga agccgccgca gtcctggcca
     2941 aggtacagtg gcccggacgg ttcgaacgcc ttgagacgcc ccccctggtg ttggacggcg
     3001 ctcataacga acatgcggcg cgcgtgctcg cgtccacgtg gaaagaggaa ttcccgggac
     3061 ggaaggcggc actggttttt gccgcatccg cagataagca catccgggaa atgattccgg
     3121 ttttgcgaga aattaccgga gaatggcacc tggttccctg cacttccccc cgcatcatgc
     3181 cggcggaaga aatggctgcc ctgctgggcg agcaggaaac cggccccgtt ttcatccatt
     3241 cctcgcttct ggatgggctg caggcggcac tggcctcccc gctcccggtc ctggcggcag
     3301 gttccctgtt ccttctggga gatttgaaag ctctgctccg ccatgcggaa aaacgcagca
     3361 ccgcccaata acccctaccg tcctttttgc ttttctccgt gacttccgac gacataaccc
     3421 tttccgccct ccggcccgga gacctgcgcc ccgaactgct ggcccccgcc ggggacatgg
     3481 actgcgcccg cgccgccgta gccaatggcg cagacgccat ttatttcggc ctggaccgtt
     3541 tcaacgcccg tctgcgcgca gacaacttca cgctggattc ccttccggaa ctgatgcgtt
     3601 tccttcacgc acacggcgtg aaggggtacg tgaccatgaa tacgctcatt tttacctccg
     3661 aactggcgga cgccctctcc tacctgggcc acctgaatgc cgccggcgcg gacggggtca
     3721 tcgtgcagga tatcgggctt gcccgctgcc tgacggaatg gggaaggcag gttcccggca
     3781 tgaagctgga actgcacgcc tccacccaga tgacactttc ctccccggac gggctggaat
     3841 tcgcctccgg attcctggac ctgaagcagg ccgtcctggc ccgggaactg agcctgaagg
     3901 aaatcgggca atgtgtacgg catacggaca ttcccctgga agtatttgta cacggagcgc
     3961 tctgcgtggc ctattccggc cagtgcctta catcggaaag cttgggacag cgcagcgcca
     4021 acaggggaga atgcgcgcag gcctgccgcc tgccctattc cctggttgta gacggcaaaa
     4081 ccgttcctct gggagaacgg cgctacctgc tcagcccgca ggacctgtgc gcccttgacc
     4141 gcatcccgga cctggtccgc atgggcgtga aaagctacaa gatagaggga cgtctgaaaa
     4201 gcccggaata cgttgcggcg gtaacggcgg cctatagaaa agctctggac gccgcctgcg
     4261 ctggaatacc tgtggatgaa atggtgaccg cacgcgaccg ttatgcactg caaatggtat
     4321 tttcacgcgg cttctccacc ggctggctgg acgggacgaa ccacccgcgt ctgacccacg
     4381 gccgctacgg taaaaagagg ggcgcctacg caggcgtcat tatgaacagc ggtcagggct
     4441 ggctggatat caggccggaa gggggcattc cgctcgcgcc cggggacggc ttcgtcattg
     4501 atgcagggga agaccgcaat gaagaacagg gcggacgtat ctggaaagtg cagagaaacc
     4561 gccttttttt ccacggaaag gcatcccgta ttgactggag cagggttaaa cccgggcaaa
     4621 aactctggaa aacggatgat ccggccctga acgcggagtt gaaaaaaatg cgggaacact
     4681 tgcaggaatc caccattccc ctccatctga catgtgccgg gtccgtcggg aaacccctga
     4741 cgattgcctg tccggaatac ggatgttccg tgcaatctcc ccagcccctt cagacggcag
     4801 aaaaacgccc tctgactccc gaagtgctga aacaacagct cggcaggctg gggggaacgg
     4861 gattccgcct ggcctcctgc gactgccggc tgccggaggg cctgatgctt ccgctcagca
     4921 tcctcaacca gacaaggcgt gccctggtgg aacgaattca aacggcccgg aaggcaaaag
     4981 aatcctccgt tccctctcat cctccggcct ccttcaccct tcctccgctg ccgaagggaa
     5041 cagccgttcc ggacatgcct ccccatttgt ctgttctgtg ccgcaaaacg gagcaaatac
     5101 ccgccgcgct ggatgccgga gcaaatgccg tttatctgga ctttgaagac ctccgggact
     5161 atgcggaagg cataaaaacc gtccgggaac acggaaaaag cattcccgtc ttcctggcga
     5221 cgccccgcat ccagaaaccc tcggaagtcg gatacttcaa acttatggaa cgggcggaac
     5281 ctgacggcat tctcattcgc aacctgggtg cggcgcaata tttccgccag tcccccctgc
     5341 accgtatcgg agatttttcc ctgaacgtcg ccaatccgta cagcgcggct atcctgaaga
     5401 aacggggaaa tctggctcat ctcaccatct cttacgacct gaacgcggaa caggtggcgg
     5461 acctgctccg cgccacgccg ccggaatggt ttgaactgac gctgcaccag cacatgccca
     5521 tgtttcatat ggagcattgc gtgttctgca ccttcctgtc gaacggcacc agttataaga
     5581 actgcggccg cccctgcgaa cggcatcatg tgcagctgcg tgaccgcgta ggacaacttc
     5641 atcccctgct ggcggacgcc gggtgccgga acacgctgtt caacggacgc gcccagacag
     5701 gtgcaggctt ccttcaggac ttccgccgtc tgggcctttc ccgtttccgg ctggaactgc
     5761 tggatgatcc cccggaaaaa gtacgccttc tggtgtcccg ctaccgggag ctgctggacg
     5821 gttcctgcac tgcggcccgg cttatccggg aattggacgt cgcagaacag ctcggcacca
     5881 cggagggcac gctccggccg ggataatctc cacctgaaca atggttaatc tccattcaaa
     5941 ttccggggtt ttgatcatac attttcaata ttttatcact ctttaaaagg atacccattc
     6001 ctctctccat gccggccacc ccctttttca ccgcgtcgct ggtaatgtct cttaaacggt
     6061 cctgcacaaa ttcatgggcc tgcatcatat atttcccatc attccacatg atgagaaacc
     6121 gtcggcagcc ccttccttat aaacagcatg ggggtttcat cccaaatacg ttatacgctg
     6181 aaaaatcaaa gctcggcttg cggataaagt ctgtaaaaac ggcaggtgcg ccagcaggcc
     6241 aaaagccgct gacgaacgcg gcatgtaccg cccgcgcctt gaagcggcgc gaaaaccttc
     6301 ttatgatggc atccatggga atgggaaact ccttttcccg gaacttccgc aagatgaata
     6361 attcccttaa agaccgcctg atccgtcaca tggaagacgg acattacgaa ccccagagca
     6421 catccgaact ggcccgcgcc ctgaacgtgg attcccggca gaagctggat ttccgcgccc
     6481 tggtggacca gatggaggag gaaggaaaac tcgtgcgtct gcaaaagggc aggtacgttt
     6541 taaaacggga gcgccggaat ctggtgcatg gcatgatccg catcctgcgc tccggcaaga
     6601 ttctttttct ccccagaaaa ggggatcccg ccgctgcggc tctgggatgg gacacggaag
     6661 ccgttccgga actggaactt aaacccaacc atctgggcac cgccctggac ggggaccgag
     6721 tggccgtgcg cgtggaacgc aaggcagcca ggggacggag gaatatccgc aggaatcgct
     6781 tttcttcccc ggatgccgac atgaaagccc gcgtggagga agtgacggaa cgcgctcgct
     6841 cccgctggct tggcgtcttc cgcaccggaa aaaacaagcc gggccgcgtg ctgggggacg
     6901 gcgtaagctc accgtcatcc attgaactgg cggaaaaacc cgccatggag gtgcttcccg
     6961 gccaactggt ttccgtggag cccataacct gcggtgagga aaagaaagcc ccgcgcggga
     7021 aaatcgtgga ggtgctcggc tatccggacg agcctcacgt ggacatggaa gccgttatca
     7081 gaaaatatgg cctttccgtg gaattccccg cctccgtgct ccgggagctg gaaacgctgc
     7141 ctcaaacccc ttcccccggg gaactcgccc gccgggaaga ctggacggac cgtaccgtca
     7201 tcaccattga cccggcaagc gccaaggatt ttgacgacgc catttccatc acggccactc
     7261 cgtccggctg gacgctggcc gttcacattg cggacgtctc ccatttcgtc aagcccggcg
     7321 gctcgctgga tggggaagcc ctgcgccgcg gcaactccac ctacctgccg gatcgcgttc
     7381 ttccgatgct cccgccccgc ctttccgacg atttgtgcag cctgcggccg gatgtcgtgc
     7441 gcctgacaaa agtatgcgaa atgaaatttg ataaaaaggg gaagatgctc cgtgcccgct
     7501 ttgcggacgc tttcatccgc agcaaggcgc gcctgaccta ccaggaggcc ttcggcatgc
     7561 tgaaaggaaa cgacaaagga gaagttccct ccacggtgcg ggaagcctgg aatctggcct
     7621 ccatcctgcg ccgcaaccgc tacgccaagg gagccctgga tctggacttc ccggaagtga
     7681 gagccgttat ggacaaggac ggccgcgtga cgggcatcat cacggaagag tacgatgaaa
     7741 gccatcagct cattgaagag tgcatgctgg ccgccaacga agccgtggcc ctggccctga
     7801 aaaacgggaa ccgtcccacc atctaccgtg tccacgaaga accggattcc gccaaacttt
     7861 ttgaattcgg ccagttgtgc agactgtacg ggcatcccgt gcacgatatc ggccaacgcc
     7921 agtacctgaa tgaactgatg aaatccatca agggttcccc ggacgagcaa ttgcttaagc
     7981 tggcgctcct gaaaagcttg atgagggcca ggtacgacac ggagcccctg ggccactacg
     8041 gcctggccac ccccaattac tgccacttca ccagccccat ccgccgttac gcggatctgg
     8101 tggtacaccg ctccctgaat cccctgctgg ccaaccctcc caaaggagct aaaggagcag
     8161 gcagtacagg caggctggag gaagacgcgg aacacatttc agaaacggaa cgcatttccg
     8221 ccagcgcgga aaaggacgca aaccggatga agctcttcga atggctggaa ggccagtgct
     8281 acgcggatca tccggaagtg cacgaagctc tggttacgga aacgcgccac ttcggcgttt
     8341 tgctggaaat cccgcgtctc cagattaagg ggctggtcaa accggacaag cttcccggcg
     8401 gccgctgggt gtatgaggcg tttgccagcc gctggaaaaa cgaccacggt tccgtgctgt
     8461 gcgcagggct gcgcgttccc gtcattcccg tgaaagtgga cagggagcag cagtgggcgg
     8521 atttcgccat cgtctcacgg gagaaaccgc ggcaaacggg aaaaacggcc ttccccgcca
     8581 aacaggagaa gggcatatcc ggccgcgggc ggcggaaccg ctgacggcaa tacgcccgta
     8641 tatggaaaca atcccatccg gaaatcaaat ccgattctcc ctgcaacaga atcaaatctg
     8701 cattttccta cacaattttt cttgaagtaa attgtatttt tcatgtatag aatgaccgtt
     8761 gtttacttgt gaatccgacc tcacgcctat gaattcagga cgctgactcc gtaagtaagc
     8821 accatatgaa tgctgaatgc caaggtccgc tcccgggtct ggagcggacc tttttcccgg
     8881 aagcctgtca tgaagaaacg ggaagggcca acaagggtgt ccaatttaca tgacagccat
     8941 tcagcacatc cccgaacgca ctccctttaa ttacgccgac ctgactgatt accgcgaagg
     9001 gcaaatcacc agtctggccc ttgtacgctc caaaggagtc aacatgacca ttctggcctt
     9061 tgacagcgag caactcctcc ctacccacca gacgcccggt cctgcgctga tacagattct
     9121 ggacgggacg gcctacttca ccgtaggcaa tgaagaagtt aatctgcccc aaggaaagag
     9181 cctcctgatt cccgccggag tacctcattc cgtcattgcc cgggaacgat tcaaaatgct
     9241 ggttacttcc atcctgccgc tggactgacc ggaaagccgt ccccaggaaa caacggagaa
     9301 ctattccggg gaattgccac ccggacatgg atctgcggca aaaccattct ttcacgagat
     9361 tctcccgaca cgggaatcgc gcagatacgc aacatgccgg aacaggagga cgggcggaaa
     9421 cggcctgctc aggcctggat cttttcccct gcggccagcg cctcaaaacg ggccagcacc
     9481 cagcagagat cggaaaggcg gttcaggaac agggccgtgt tcacgttgct cagggcatga
     9541 tccagcctca atgccaccac acggcgttcc gcacggcggc agacggaacg ggccaggtcc
     9601 aaatatgcgg atcccagcga tccttccttg cccgggcggg cccagccttt gaaacggatg
     9661 tcgcattcct tttccagcaa atgggcctgt tcctccagcc aagccacatc ctccgcctta
     9721 atgcgggcgt accccttggc gtcatatttg ggcaaatcct cctccatcgt ggccagttcc
     9781 cccatggcac ccaccaggcg agcctggacg ccgtccagca tctccaccgt ccgggagctt
     9841 accccggaat ggcgcaccac gccaatcagg gaatttaatt catccaccgt tccgtaagct
     9901 tcaatgcgcg gcgctgtctt gggactacgc ctgccgaaca tcaggtccgt ttctccgctg
     9961 tctccacgct tggtgattac gctcatacca tgtaccctac catgcccgcg ccgtcctgca
    10021 cgcaaaaaag aaggcatatt ccggagcggg agacagcgcc atctttcccg gcatgtaccg
    10081 cactctctat tcttttgaca tgcaataaaa actatataag aagaatgaat aaatgcttct
    10141 tcttcgtctt cctgccgttt tcctccctct cttcatcagc ttcctcttcg gggcatgctc
    10201 taaaaaggaa agcgccttgc tgcccggaga aataatcgtg aacgcatcgg aactgcccgg
    10261 ccccatacag gaatggaaga cggattcggc ctgtgaaaga tgcctgacca tcaatctgga
    10321 caatccgtcc cttccctggt tccgcatcgc tgagggcata tcggaggctt atgcgcatgg
    10381 cgtgaataat ctccgtttga aaagagggga tgccatcgcg tccgcctttg aaaatggaat
    10441 ttgctgggat cttcccgtct ccacggatat ggccccggaa tctgatccag aatctgaaaa
    10501 agacatgaac ccggacattc cctgccttcc cgacgatgcc gttctggaac atcctttccg
    10561 tgaagatgac tttcttgttt tcaacaaaac accttcccgg aaacccgttt atcccatcgt
    10621 gaaattcatc cggtcccctt acccggaaag agggaactac cagcttgttt actggcgtat
    10681 ccagaaaaaa ccatccggtc catcccggaa aaacgacctt caggctttcg ccaatatggc
    10741 aaatgaacat atcaaccagc gcagagcatt cattttcctc attgagaacg aaacaccctg
    10801 cggagaagta ttcgactatc tgaatgccat tgacgggaga gacacgctgg tgggcgtaac
    10861 cttttcccgt gaaaatgaat aacgtctccc aacaaggatt catcagggct ttcctccccc
    10921 cgctcatcat catgacacgt caggccgttt gaaacttttc ccaacggctg tacaacccct
    10981 gcatgactcg gacaagagat gaattggcgg cggaaaccgc accatccgtc aaagcgcctc
    11041 tctggacgcg agactacatc ctggcctgtt gcgccaactt aatgacgggg ctggccttct
    11101 atgaactggt gcccgttctc ccactgtacc tgacgggaca attgcaggtc tcctccggct
    11161 ggctgggctg gatcatgtcc atttatgttc tggccgccat tatctcccgc ccctggttcg
    11221 cacaccgggt ggataccgga gaccgcaaga agatttacat tacggtttac attatgctgg
    11281 ctctgtcctt ttcagggtat gctgcggccg ccaccgccct ggcgtttttc ctgacgcgct
    11341 tcatacaggg cctgatctgg ggaggcatga ccacctccgg cccgacaatg gcggtggata
    11401 tcatcccccc tgcccgccgc ggggaaggcc tgggcttctt cggcatgaca atgacgctgg
    11461 gnnnnnnnnn ncatgtgcct gggccccgtc atcggccttc aggtatacca gcagtacggc
    11521 ttttacgtga ttacctggag ctccctgctc ctgtgcctgg ccggagcggg cattgcatct
    11581 ctcatccgcg ttcccaggag ccctgttcag gaccccgtgt atgaaacgcc taaaaaggta
    11641 ctggaccgtc ttgtgctgcg cgtgggcatt ccgctggcgg tgaacgtgat gatcgccacc
    11701 ttctcttatg gcgtggtggc cgtgtattcc gccctgtacg gggaaatgca cgggttcaaa
    11761 tacgccgggc tgttttacgc gctgatgggg ctgggcatgc tggtctccag gttcatggta
    11821 ggcaggcaaa tcgaccgcgg acgcgtggcg gagctttccg tcatctccct gagcatcctg
    11881 accgtcagtt tcggcgcgct ggcgctggcg ccgctggaat ggatctatta cgcttccgcc
    11941 atcctcatcg gctttggctt cggcattttc atccccacgt tccaaaccat gaaactgaac
    12001 atggcggacc gcggccaccg gggagccgtc aattccacat ttttcaccgc ttttgacatc
    12061 ggggtgggaa caggcatgtt cctgggaggc aaaatttacg cgtacctgaa cctgaactgg
    12121 gccttcggaa cgggagccct gctgaatttg ctggcgatcg tatattttta ccgcatctcc
    12181 ctggaccact accgtaaaaa taaattgggc gtggattctg attgattttc cttccatcac
    12241 tcccgttccg ggatggaaaa aacatcaatt acccgtttgg ctgaaggcct cacgcgcagc
    12301 aaccgcggat tccaggccag ggaaatactg cgggacagcc cgtccagcgc cggatgagta
    12361 accttgacaa acccttcccc cagttctttt tccgccatgc agggcaggat tgccatgaac
    12421 gctccgctct tcaacatgcg ggccgcaaaa ggaaaagatt gcgtttcaca gtgcacccgc
    12481 agcctgatgc cgaactcagc gcaattcctt ttcagcaacg catgaaaccc ggaaccgctc
    12541 cccagcgttg ccagggggaa catttctagc aggcggagaa aatcctcctt cttcccccgc
    12601 gcctttttcc cggccagggc tgcaggaacg tacagcgcat aatccatgga gcaaatgacc
    12661 cggctttcca acccttccgc ctccagcgcg ctggtacgca caatgccgaa gtctatatcc
    12721 atattctgga gcttagaggg aatctcactg ttgcggaggt tctccagcgc gaaaagccac
    12781 ggcagccccc gtttctggat actcgccagc acgggagccg ccacccaagc atgcaggctg
    12841 tccccggctc cgatagtgaa ccggtagggc agattccggc tccggctttt aaaatcatcc
    12901 agcaggcaca ggctttcact ggccagcctc accagctccc acccggctcc ggtaagagcc
    12961 agcactttcc cgcggcggct cgccagttcc acaccgaaga attcctccaa ttccttaatc
    13021 tgccgggaat acaggctctg ccgcgcacta tccccccggg cggcagcggc aatgctcccg
    13081 gccttggcca cttctatcaa ggttttcaat cggtccaggg acagtccccg ttctgcgaaa
    13141 agatgctcaa acataacttt atagttatat aatatgaata cttacataat gcaaaagtca
    13201 acaaatgcat tatggtgaca aggtcacagg gcgcgaaggc gctttccgca tatctccgcg
    13261 cccctcaacc aaaagaataa aaccatgatc accattgacg gaaaatacac ggaagcccgc
    13321 gtattcacgg atgaactgga agacctggcc cgcacccaga tcagggacgt ctgcaaccac
    13381 cccgcctttg aaggaagccg ggtgcgcatc atgccggacg tgcacgccgg agccggctgc
    13441 gtcatcgggt tcacggcgga attgaaaacg gataaggtaa ttcccaatct gatcggcgtg
    13501 gatatcggct gcggagtgct tacctccaga ctgtccggac tgccggattt ttccagcttt
    13561 gacgcacgcc tgcgcgagcg cgtcccgtcc gggatgcatg cccgtccctc cgttcatcaa
    13621 gccttgctgg acgacccgga actggatgaa gaaatctccc gcatctgcat ggacgtgctg
    13681 agaacggaca aagacaggca ccgccgctcc gtagggtctc tgggaggagg caaccacttc
    13741 attgaaatag cgcaagggaa agagcacgct ttcctgtgca tccactccgg ttcccgcaat
    13801 ttcggtctga agattgcgga acgctaccag aaaatcgccg tggatacttg tccggacgcc
    13861 tatcttaaag aacgcaagaa aggactctgc taccttcagg gagaaaatac gctgaactac
    13921 atgcgggata tgaaggtggc tcagcgcttc gcagccctga accgccgcgt gatactgcat
    13981 gaactgctgg atgatgcagc tgatctggaa agtttcgaca ccgtgcacaa ctacattgcg
    14041 gaagaccaaa tcatccgcaa gggagccgtc cgggccggac gcggagaaaa actcatcgtt
    14101 cccctgaata tgcgtgacgg ctccgtcatc tgcatcggca aaggaaatga agaattcaat
    14161 tcctcatctc cgcacggcgc aggccgcagc atgtcccgca aaaaggccaa agccaatctt
    14221 tccctggagg aattcgaggc ttccatgcag ggtattttct ccacctgcgt ttcccgcgct
    14281 acgctggatg aagcgccaat ggcctacaag gatggcgaga gcgtgctgga caacatccat
    14341 gaaacggcgg aaatcgtgga acgcatcagg cccgtatata acttcaaggc acccgaatag
    14401 caggcgtccc aatacccgcc cctcccggaa tatgattttc cgggaggctt ttcccaaacc
    14461 tccaaaacac ggtcaggaac gctcggcagc ataaatcaac ttataaggtt ccgccgtctg
    14521 aatcgggatg gcgtacactt tagaggtatc attccattcc acgccggaaa cgtcaaactt
    14581 ctgacggtca taataaccta ccacctttcc attggtcact acacagaaag cggctgcatt
    14641 ttccccattc cgcacaataa tcatgttatc ctttttggag gcgcttacag acgggctgag
    14701 ttttgcgttc tgcttgtatt tggtccaata gccgttgagc tgggacccat gcagattctg
    14761 cccccacaaa ttgaccgtct ttccatgctg atgcaggaag gcaatgcgga atttcggttt
    14821 ggggtaccgt ttggcggcaa tgcgggcctt ggatgcccgg gccatttcct ctgtcatttt
    14881 aaaactgtaa tccccatatt gatgcccctt gcggtcaatc gggacaaacc aattaaaaat
    14941 ttcaaagtca tcggtaaagt ccgtcctgct gatattgcaa atgcgttcat acaaggccaa
    15001 atgggcttcg gaaacctcat acgcctgcct ggaaagcggc ttgcgccgca atgattcaaa
    15061 cagcctagga taaaattcgg gattgattcc cagcttatgg aaatacagat agaaggaata
    15121 ttgagcaaaa ccccatttgg cccagccgtt caaatcatgg aacggcatgc cctttaccgc
    15181 ttctgaaagc aggtatttgc acgggttttc catatcggag gcctgctcat tgccattaat
    15241 ggcattggtc acctggtcca gaatcagctg ggcaaaaaaa ttgttggaac tttccgacat
    15301 gctgcgccag ttgaacggat gctggtagca atgaccgatt tcatgcccga ttccccaaag
    15361 gcggtttccc tgaagaactc caggagcgag cacttcattt ttcagcgtat ttccctgatc
    15421 tccaatactg tagccgcagt aataccaggt tgcaaacata tgggctcctc cttctacgga
    15481 aacgccaagc tgccggttat tgtgcggctg gggatacttg tcaaatccca tgattttcca
    15541 catggccttg acgctttcat cccataccct gacggatttc gtaatggaat ccggactgta
    15601 atctttcagg tactttgtgt gaagaatcat catggaattc cgcccaacga tatcgaacat
    15661 gctatgcggg gcgttgctga tcatacgctt ccagtcctta tccgtgtgcc tcgtcacgtc
    15721 aaaatatcca ttcactatgc cgcttcccac aggaatatgc accgttatcg gcttcttggg
    15781 cgtgctgata tcggtgtgat acatcacgta aagcaatccc gaacggttaa ttctaatttt
    15841 gtttacccct tcattcaaag gataggagga agactctatt cccgctggag aaacggaggc
    15901 cagcccaata tcttctccat gggtaggtcc tacgaacaca agaatttcat ctcctttctc
    15961 cgcgacaatt ccggtaggat tgtcaaattt gttcagggcg ttgatatttc tcaccttcgc
    16021 agcaaactcc ggatggctgt aagccttata ggaagcaatg cggaattctt tgtcttcata
    16081 atcgccggat tttattttct tcgcaatcat ctgcaaaaac tccggaagag ccttgaggtc
    16141 cgtcagggta cacctgggat tgatggagga acagctcgtg tccttaaata ctttcagcaa
    16201 tttccggtta agtgcggact ccaccctttt gtaaaagctg atttcccgta cggtaaaaga
    16261 cggagaggaa ccttcgggat aatcaaccgt caacctgatt tttcgaacct tttgaagagg
    16321 ctttttaaat ctgatcatcc tggagccgac gatatcgaat tttccaaggt ccttccaaga
    16381 cgagccggac atggcggaca cggcaatttc attgaaatcg gctccggaaa aaatgatgta
    16441 atgaatggtc tccggcactt caaacacaaa ttccccctcc acatgatgca ctccgggaac
    16501 atgccagttg gcggattcca tagagccgtc cacagcctgc tttacggaat gattgccgga
    16561 atagggggac tgggaggaag cagcttcttt cacgtggagc aaaatgtctt ccggaacatc
    16621 cagcgaagga tatttttccg cgagggattt atttacggag attgtcagaa aaaggaaaaa
    16681 taaaaaaatg gaaagaaaaa tggaatcttt tctcatacac tatattaata tttaat
//
LOCUS       DAWEMX010000047        16549 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1460_length_16549_cov_14.511337, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000047 DAWEMX010000000
VERSION     DAWEMX010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 16549)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16549)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16549
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1460_length_16549_cov_14.511337"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    274..283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10747..10756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3354 a         4326 c         4987 g         3862 t
ORIGIN      
        1 acctgttcag ctccaatccg gcggatgccg cccggtttta tgccagcatt gattttgaca
       61 aggtgccccc gaagatgcgt gcggatattt tatataagag cgcatgggcg attgcccagg
      121 ccgggaacag aggtgtggcc gccaagctgc tgactgattt tatcaatgat tttccgaaag
      181 atccccgcat ttgcgaggca ttgacgttgc gcggggacat gtatgccaag accaagaagg
      241 aagctgaggc gctgatggat tttgaccgtg tgannnnnnn nnnccgccgc atggcagaga
      301 gccgctcaga tttatgcggg acgccaggat atggcgaaca tggcgaagta ttatgaaggc
      361 ctgattcaga attttccgaa ggcgtctcct gccgctctgg ctgaagccca tttcctgctg
      421 gggcgtgcgg cgtttgacca gggagatttc aagtcttcta tcatccatat ggcggaggcc
      481 aagactctgg atccccagaa atatggagaa caggttaatg tgctttccgt tctgtcctat
      541 cacaagctcc aggacgtgaa taaactgaag gaagccctgg aaaccctgca aaaggaaaat
      601 ccttccgccg tggctcgtgt gccggatgtc atccccgcat ggctgggcct tcaggcctat
      661 gggatgaagg atctggaaac ggcggacaag tatatgacct gggctacgca gaacgaccag
      721 cttcagaatg tgaagaaggt gatttggcgt aatttggcga aggtgcgcct ggcgctcaga
      781 aagtatgacc gcgccttggt ggcttccaac aatttcctga aggatgagga ccagccttac
      841 cgccgtgcgg acggcatgct ggacaaggct tccattttgc tggggctggg caagtatgcg
      901 gatgccagga agacggcgga agatgctctg gctctgggcg tggaaggtcc tctgatggct
      961 tccttgaaaa ttgttctggg ggatatttcc tatgcggaga aaaagtttga tgaagcggcc
     1021 aagcattacg gtgttacggc cgagctgttt gtcaatgacg ccgaactgaa acccaaggct
     1081 ctgttcaagg cggcggaagc tttggacaag gccgggcgca aatcggaggc ttcccaatac
     1141 cgggcccgcc tgcaaaagga attcccggat tggaaacagg atggagagtc tttgcctccg
     1201 gacgcacgat aagcgtttta aggattgaaa ccggaaatgg agaacaggcg taaaagatgg
     1261 tatgatctgt ttggattgct gcctcgcaat ctgggccgtc tgttgctgtg gttcagcctc
     1321 attcctatcg ctttcattat cgttcttctc gtttatgcat ggagggcgga ccagtatgag
     1381 ctggacgagg ttttggcgcc gttggaaaat tgcgccgcat atgatcgcaa cggccagtgg
     1441 ataggcacct tgacagatca tgaccgggtg tatgtagcgc gcaatgaact gcccgataat
     1501 ctggtgaatg ctttcgttgc gcgggaagat gaagactttt tcgaacatgg cggcattgtg
     1561 tactcttcca ttatccgctc catttgccgg aatttgacta cattgtccta tgcccagggt
     1621 gcttccacga ttacgatgca gctggcgcgg aattgttatg agctgggagg gaagacccta
     1681 gatcgcaagg ttttggaaat ggccgtggcc cgccgcattg agggcaagta ttccaaggat
     1741 gaaattctga cggcttacct gaaccgcatt tactttggcc agcaatgcta tgggattgcg
     1801 caggcggcgg atttgtattt cggcaaaaaa gtagcggagc tgacactggc ggagtgcgct
     1861 accctggctg ggctggtgcg cggaccttcc atctacaatc ccgtttacag cccggaggct
     1921 gctgccaagg tccgcaatgc gacgctggaa aggatgtttg agtgtgaatt cattacccag
     1981 gaacaattat ggcaggcgct ccgcgaaccg atggcggtgg ctggtaaacg ggaagctcgg
     2041 cccggttctt atcccgttct ggtcgtttcc cgtgagttgg gagacctgga ttgctgtgac
     2101 gagcaggagg gaacctccag catttttgta atgaccacgc tggatttgga atttcagcgg
     2161 atggttgaag atgtaagcga gcctatgctg gtcgctctgg aatcttcttc cgtgtgggca
     2221 gggttgccca aaagggtgga caaccagttg aatcgttgtg tgcaagcggc catattgtgt
     2281 gtggattccc gtaaagggga tctgctggcc gtaacagggg ggcgttccgc ccaggacggt
     2341 ctggaccgat ggcagtccaa ggtaatgccc ggcgatctgt tcactcccat tgtcaattta
     2401 tgcgccgtcg accagcgtag aaccgttatt cgcagcaatc cggaagtcac tggccgcggg
     2461 gtaggtttca atacagtaat agaaacggca caaaaggccg gttacaaagg ggaacttccc
     2521 cgttcttcgg acttgtatac cggcaggttt tccgtgcctc tttcgcatgc cgtcaatgcc
     2581 ctgtatctca tcggcaatga cgggctcaat gttagtattt cctccgtcag gcaggtgggt
     2641 accaccaaaa agaacctggt gatggtgaat gccccctctc cggaagaaag ccgccgggaa
     2701 atcctgccta gggaatccgc ccacctggtg gcgtcccttc ctcccttccg ctacgacgaa
     2761 cggaccagga aaaccttcgt caatgtcagg cttcctggaa atacggggca tttttccgcc
     2821 gtcatggggc gttatttcac ggtttttgcc tgggtgggct ttgattctcc ggaggccgcc
     2881 gtctatgaaa aaaaaggggt ttccgcagcg ttggcaaaaa cctgttcctc tctggccggg
     2941 gaggtgtatg accgtgcgga ggaaggtcgc agaaaagctg tccgggaacg ccgggagcgg
     3001 gaaaatgctg cagaacaggg ctcccaatga agttcaattt tccattagta gaagcatcat
     3061 gagacactca cttgaaatga ccacggccat ccaggccgcc aaatccgccg gagctttcct
     3121 gaagaagcat ttttatgagc aaaagaaggt ggatgaagcc agccagaatg atatcaagct
     3181 ggagctggac aagctttccc agaaactgat tacggaggaa attctttccg tgtttcccaa
     3241 ccacgctgtt ttgggagaag aaggatacac cggggacagg aacagcgagt atgaatggat
     3301 tgtagacccc attgacggga cggtgaatta tttttacacg attccctggt tctgcgtctc
     3361 catagctttg cgccgccgcg gggaagtggt gttgggcgtg atttacgatc ccatgatgga
     3421 tgaatgctgg catgtggaaa agggcgggat tccgtacatg aatgatcatc ccatgcattg
     3481 cagccggcgg gaacgcatgg cggaagccgt ggtattcgtg gggcacggca agacggacgg
     3541 ttccaaggaa aaggggattg aacgtttcgc caggatcgcc tggcaggtac gcaaggtgcg
     3601 caataacggt tctgcggctc tggccctggc ttatattgcc tgcggcagat ttgacgccta
     3661 tgtggaaagc gtcatatcca tctgggatgt cgccgctggg gttttgctgg tggaagcagc
     3721 cggcggcaag gttgttttgg aaccgaagga agataatccg gaacaattcg ccattgtcgc
     3781 gtggaacggc cgcatcccta tcatggaggc gctgggagag taacctgaac attattgcca
     3841 cagtaccgta tgaaaaacat gatcgctttg accgggatcg ggtatgatat ccaccgtttt
     3901 gcggaagttc cgcgcccctt gatgctgggc ggtgtgcata ttccttcctc ccgcggcctg
     3961 gacggccatt ccgacgcgga tgttttatgc cacgccattg cggatgccct gctgggcgct
     4021 gccgggttgc cggacatcgg ctactggttt ccgccgagcg atccttcctg caaggacatc
     4081 ccttccctgg atattgtggc gaaagccgtt tctctgattc gggagcgcgg cggccgcatc
     4141 gtgaatgtgg actcgtccct tattgcagaa tctccccgta tttctcctta tttggagcag
     4201 atgaagggag ctttgggcgc cgctctggga atctctccgg tccgtgtggg catcaaggcc
     4261 actactaatg aacagcttgg cgcgctgggc cgcagggaag gcattgccgc ttttgccgta
     4321 gcggctattc tgatgccgga ggatttgacc atgccgtaag gagaacgctc cggttccgtt
     4381 tctggaagcc ttttttcaaa aaaggaaacc ggtacggggt tttaaacaag gaatatgctt
     4441 cagaaataac gggcaggtgg aaatggcgtt ttttgggggg agaccctttc ccaggcagtt
     4501 ttaatgaaat ggcaggaaat tgttaaccgt tccctggcgg caaagccgtt tttggctgca
     4561 taataatatt acccgtttcc ctgcacaggg aagaggtgtt gcctgatatc tcagctgatg
     4621 cagcggtaat aataatccac gcgggagatg aaccaagacc atttgaagcc gggtttgcca
     4681 ttgtccagca gaaggtgggg gcaacgcttg ccggtccagt agtaatgggg cacgacattc
     4741 cgcaggggaa tatcgtgggc atacatgttg taggccgcca gcttggcggc gcggtcaaag
     4801 gttttgacga tgctctgtcc tcgcttctca cacatttcaa tgccgatgga gtacatgtct
     4861 ccggggccgc cccggtctgc atggcgtccc gtttcattca gggggatatg ctgcatggtc
     4921 acatacgggt ctaccgtata atgccagttg cagcgcaaag ctccgttatt cagggcgcgt
     4981 gcatgctgca tggcgtctgc ggacgggttt tccgtgttgt gcatggtgat gaagcgcggc
     5041 ttcattcggg aagaacggcg gcggagcgga gaagatttag gcatgaacat cggccggacg
     5101 ttacattccg ccagcaattg gcgcggagaa cgtttcacca cggggacccc ggagggctta
     5161 aaggagatca tgggagccat tcctgctttt ccggaaggtt ccgaacaact ggtcagggca
     5221 agcagggcgg cggtttccag cagaaaagtt ctgcggtcca tgccctggac tttctccagc
     5281 atgggctgga gctgaccgga aaagcgggca agataagtgg cgatggtgga aatattgctc
     5341 atacggaggc cgggagatgg ggaattttca gcacggatgc gggaaaagtt aagttcacgc
     5401 taagattcgc tcttcttctt tgcaacgaaa aagtgtgaaa gcttcagcat tgtgccgaat
     5461 tcttcgctct ttttgtcata ttggtttgct taaaccgttg ttgagagcag aacagtctct
     5521 gcttctgcgg cggtgacggc atccgcgcct gccggggaca gaatgaattc tcccttccgg
     5581 gccgttttcc cgtcccagag cacggaaccg cagaggatcg tgaagacggc aaagcgggag
     5641 gagtccggct gggtaaaccg ttctccctta tccaggcggt attcctttgt ggtgaaatac
     5701 ggcatttccg tcaggtatcc ggggcgctgg ctgagttccc ggaattcccc gatggaatcc
     5761 aatgcctgtt ccaggtgcag ttcccgtcct cgtccggagt catccgtgcg gttccagtca
     5821 taaaggcggt aggtggtatc gctattctgc tgaatttccg caatgaggtg cccggcgcca
     5881 atggcgtgaa ccagccctgc cggaatgtaa aggtgttccc ccgattccag atgggtggag
     5941 cgtatcagct gctccatctg tcctgtttcc gccgcatgac ggatgtccgg gagtgtggcg
     6001 ttttcctcca ttcccccgta aatcagggcg tccggggatg cgtgcagcac ataccagatt
     6061 tcatttttta cttctccctg ccgggctgcc gccgtgcgtt ccgggggatg cacctgaacg
     6121 gaaagatttt cgcgggcatc cagaattttg cagagcaggg gaaagcgggg gaaacgctcg
     6181 tagccaggac cgaaaatttc ctgccgatgt tcttcccaca gccggtgcag gggcattcct
     6241 tcccattcgc cgtccttgac gatactcatg gcttcaggac ggtcgctgat ttcccacgct
     6301 tccccataag ctgttttccg gtcgggcagt tcccgtccca gcatggttct catgctctgg
     6361 ccgccccaga tgcgcggctg gtagatggaa tggaatcgaa aaggctgcat ggttgtagcg
     6421 gaagcgtagg atatctttta tggacgggca agcaaaatgc ccacagggtt tcctgtgggc
     6481 atggagagag atgaagacgg agaattgcct gttatttgat ggattcccag acaatgccga
     6541 tttcgtcaaa tggcacgtgt tccttgttct tgtccggttt cggaacgccg tagttggtcc
     6601 ggaacagggg caggatggga gctcccgttt taggatcctt gctcatgggg gccattccaa
     6661 cttcttcaag cagaagagtc atgccgaatt ccccgccggg aatttcgctg atcagaatct
     6721 caatgggata aacattgcct tgcttcactt tgaaggattt gccggcaacc acgcctccca
     6781 tggtccggtt ccagtggccg gaagaggagt atttgtagaa tgtaacagga gggacattga
     6841 ttccggctgt tttgagctct tttttcaggt cgtcgtaacc gggcttgttt tccatggctg
     6901 caatccattt ggtagatgct gtctgttttc ctaaggaagc ctggaaccac ccgtagtcaa
     6961 atacgttttc attgttgaat cgtacggcaa taacgtcgtc gcccgctccc acaaagcgga
     7021 atgttccgga tttcggagcg cgcactttgc cacgatagat agcaatccat cggcttggct
     7081 tgactttgtc cgcacatccg taagctttcg gagcttcatt ggcggatgtc cggggcacgt
     7141 aaaattgggc ggcgtacagc tgctggggag atttatagaa acggtttaaa tctttttcat
     7201 cccaaccgtg ggtgacaaag cgggagagga tggagcagaa ctggtcttgg gacaggttgg
     7261 taggacgtcc gccaggagtc tgcttggtgt cgtaaaaggt acctaccagc gtgcttccgg
     7321 agggttggga cgtaccgaaa gcagaggtag agtctcccag gtcggggccg aagccgtccc
     7381 ccatgccgag ggcggttccc tcctccagca tgacaggttc atcgggcggg tcaatgtcga
     7441 tcttggggag gctcaccgga gcggcggccg ctgccaccac gaccttgacg ggcgtctccg
     7501 catgggagga agaggaagtt tccctctgta cttccggcgg cggaggcgta tccgacggcg
     7561 gcgcatcgtc cggcgtgatg tagccgacga ctttgggttc ttccggaggg gcgatgatga
     7621 tggtcaggga gtagagaatg gctcctgcca aaacgacgga gagaatggat gtcgctaatg
     7681 cggcaatata atttttccgc ctttccttgc gcagggtttc cactgcgtct tccgtgggtt
     7741 ctatatgaag gctcatgcat gtatgatagc acaagaatcc catatgttga gtaacatgag
     7801 gataacaggg ataacatgtc ggtaacaaaa aaggcggcct tgccgtcctt tctccgtatg
     7861 ggcttttcag cgtttcctcc gtctttccgt ccctcccggt gccagcgcaa tggcaagggt
     7921 ggagcagatg cccagaatca ggggataata caggtatttc atgatcagca ggggagaaac
     7981 ctgggagatt ccggcggcca tgagcatttg agccccgtag gggatgactc cctgaatcag
     8041 gcaggaaaag gtgtccagaa tgctggcgct gcgtttgggt gggatttgga atttgtcgct
     8101 tatttccttg gcgatggggc cagccgtgat gatggcgatc gtattgttgg ccgtgcagag
     8161 gttggccagg gaaaccagcc cggcaatgct gaattccgcg ccgcgcctgc tgtggatatg
     8221 ggccgtaatt tttccgataa tatacgcgat acctccgttg aaacggatga tggacagcac
     8281 gccgccggcc agcagagtga cgatgatcag ttctcccatg ccggtcatgc cgctgcccat
     8341 ggcctggaac caggccatga aggagaaata ccccatgccg atgccaatca gtcccgttgc
     8401 aaggagcccc atggtcagaa cggccatgac gtggattccg gccagggccg tcaccagtac
     8461 gagcagatag gggaatacct tcatccattc aatggagata tccgctgtgg agggggactg
     8521 cacgttccat ccgagcagga tgtagcaggc cagggccagc agggcggcgg ggagcgccac
     8581 ctttatgttg gctcggaatt tgtcccgcat ggcgcagccc tgcgtacggg tggcggcgat
     8641 cgtggtgtct gaaatgaagg aaagattgtc tccgaagaat gctcccccca ccacaatggc
     8701 cgtcatcatg ccggcgctga cctgcgtggc ctgtgccagc cctgtggcta cgggagccag
     8761 agcgacaatg gtgcctacgg atgttcctac ggagagggag atgaaacagg aggcgatgaa
     8821 cacgctggca aacagcagat tgtccggaag aagccgcagc gtaagctgca cggtggcgtc
     8881 aatggctccc atatccctgg cagactgggc gaaggctcct gccagcacga aaatccagac
     8941 catcatcagg atgttccggt tggctactcc ggaggaaaac agatccaccc gttccgccat
     9001 gctttttccg cgggtgatga aaacggccca gacagctgcc gccataaagg ctaccgtgac
     9061 cggcacagcg taaaaatcct gtacggctat ggagatgacc aggtaaagca ggaagaaaac
     9121 cagcaggggg ctcagcgccc acaggctggc tttggcgggg ggcgtatcct ggttgtccgg
     9181 ggggctggtc attggagtga ggggagctgg ccgaaaagtc agttgccgtc ggcttccatc
     9241 cggctcagat accaccggta aatgcgttcc actccctctg ccagctccac ccggtgtttc
     9301 cagcccaggg agtgcagctt ggagacgtcc gtcagcttgc gcatggtgcc gtccggcttg
     9361 tctgcgttga acgccagatt tccccggtac cctgccgtag cggcaatcag gcgggccagt
     9421 ccgccgatgg agatttcttt cccggtgccg atattgatgt ggcagttccg cacttccctt
     9481 cctccttctt gcagctggct gaaatccact tgctccatca catggacgca ggcgtccgcc
     9541 atgtcttcgc tccacaggaa ctctcgcagg ggagttcccg taccccacag ttccacggaa
     9601 ccgggggtaa tgccgtattt ggccagcacg gccaggattt ccttttcttc agccgtgccg
     9661 tccacctgtt ccactgggcg cgcgttcaga tctttccgca cggcgttcca gtctccctcc
     9721 atcaggcact tggccaggtg tatcttgcgt accatggcgg gcagcacgtg gctgttttcc
     9781 aagtggaaat tgtcattggg gccgtacaga tttgtaggca tgacggcgat gtagttggta
     9841 ccgtattgca ggttgaagct ttcgcacatt ttcagccctg ctattttggc aatggcatag
     9901 ggttcgttgg tgtattccag cggggaggtg agcagggcgt tttccttcat gggctgggga
     9961 gcttcgcgcg ggtagatgca ggtgctcccc aggaagagga gcttggatac gccatgccgg
    10021 aagctttccc cgatgacgtt ctgctgaatc tgaaggttgc ggaagatgaa gtccgcccgg
    10081 tagcgggagt tggcgatgat gccgcccaca aacgccgctg ccagaaagac gtattccggc
    10141 ttttcccggt caaaaaactc ccggacggcc gcagggtctt ccagatccag ttctttgtgc
    10201 ggccggccaa tcaggtggga atagcctttg tcttccaggg atttccagat ggcggaaccg
    10261 acgaggccgc ggtgtccggc cacaaagatt ctgctgtttt ttttcatctc agaaatgaac
    10321 gggagttttt acgtatttca tgtcgtggcg caccatgatg cgcacgagtt cctcaaacgg
    10381 agtggaggtg gggttccagc caagaagggt tttggccttg gttgggtctc ccaacagttg
    10441 ttcaacttcg gcagggcgga aatatttggg gtccacttcc accagaatat tgcccgtctt
    10501 cctgtcgcgt ccgcgttctt ccacgccttc tccctcccat tccagctcta ttccggcttc
    10561 acggaaggcc agcgtgcaga attccctgac cgtgtgcatt tccccggtgg cgatgacgaa
    10621 atcctccggt gtgtcatgtt ggagaatcag ccacatgcat tccacgtaat ccttggcgta
    10681 accccagtcg cgcagggcgt tcaggttgcc caggtagagc ttgtcctgca tgccctgggc
    10741 aatgcgnnnn nnnnnnattt tgcgggtaac gaagttttct ccgcggcgtt cgctttcgtg
    10801 gttgaacagg atgccgttaa cggcaaacat attgtaagat tcccggtaat tcttggtaat
    10861 ccagaatccg tattgcttgg ctacgccgta gggtgaacgg ggataaaacg gcgtggtttc
    10921 cttctggggg acttcctgca ccagcccgaa aagctcggac gtggaagcct ggtagatgcg
    10981 gcatttcttt tccatgccca gaatgcggac ggcttccagc atgcggaggg ttcccacggc
    11041 atccgtttcc gccgtgtatt ccggtacgtc aaagctgact ttcacatggc tctgggctgc
    11101 cagattgtag atttcatccg gctgcacggt ctggatgatg cggatgagtg aactggaatc
    11161 cgtcatgtcc ccgtagtgga ggttaatcag gcggtttttc ttcatgtccc gcacccattc
    11221 gtccaggtaa aggtgttcga tgcggcccgt gttgaaggag gaggaccggc gcaggatgcc
    11281 gtgtacttca taccctttgt caatcaggaa ctctgcgaga aaggaaccgt cctgtcccgt
    11341 aatgccggag attaatgcga ttttgctcat atggtgtgtc aggttgtttg ctggcgggag
    11401 aggttgatga aataaaagaa acctctcccg gagccgcaat tcatgggctc cgggcataaa
    11461 taacgcattt ttcccagcga ggcgaggaaa atgctctggt ccggaggaag ggggcggagc
    11521 cgtcagcggg catgttgccg cttgctggcc gcaatcattt tcctgaaatt ccggaacaga
    11581 tactgggggt ccctggggcc gggagccgct tccgggtggt attgtacgct gaatacggga
    11641 agcgtcctgt gggcgatgcc ttccacggta ttgtcattca gattgatcag cgttatttcc
    11701 acgtcgtccg gcacggtgtc cggatccacg gcaaaaccgt ggttctggga cgtaatgctg
    11761 attttacccg ttttcaaatc tctgaccgga tggttcgctc cacggtgccc gaacttcagc
    11821 ttgaaggttt tcgctcccag cgcgtgggaa ataatctggt ggcccaggca gatgccgaac
    11881 atgggcactt tcccaatcaa tgcagatact tcccggtgaa tgtccgtcag cgtggcggga
    11941 tctcccggac cattggaaag aaagacgccg tccgggtcca tggcaagcac ttcctccgcc
    12001 gtcgtgtggg cgggaactac ggttacgcgg aagccgtttt ccctcaactg gcgcaggatg
    12061 ttgtatttaa tgccgaagtc aaaggcgacg atgtcgtatt ccgcaggggg cagagttttt
    12121 cccgtaacca gcgcgtccac ttcctccggt gtcatggggt gggaaccgat tttgtccacg
    12181 atattgcagc cttccatgga gggggcgttt ctggctgctt ccacggcttc ctccggagtc
    12241 agctccgtgg tgaggcaggc gcgcatggct ccggcggagc gcagatgctt ggcgattttg
    12301 cgggtatcca ccccttcaat gccgatgacg ccctgttttt ccagccacgg gcccagggat
    12361 tccgtggcac gccagttgga gtggactttc gccagttccg ccaccacaaa gccgctgacc
    12421 tgcgctttgc ctgattcccc gtcttccggg ttgacgccgt aattgccgat cagaggataa
    12481 gtcatggtga cgatctggcc gctgtaggac ggatccgtca atacttcctg atagccggtc
    12541 atggacgtgt tgaagcaggc ttcccccact tccgtgcccg tggccccgaa gtgcgaaccg
    12601 atgaagacgc ttccgtcttc cagtgcgaga atggctctca tggtgatgta agtatgctta
    12661 ggtggtgcgg gattaaaggt tttgggcgtg gtactcctga atagtgcaca cctgaagatt
    12721 tttgttcttc agcgcctcaa tggcattcac gcaggcctgc gcggaagcca ggttggtgca
    12781 ataggaggtt ttggcggcaa cggcggagga acggatgatg atgtcgtccg cccgcgcgtc
    12841 gtgggagccg ggggtgttga tgatgaaatc aatatccccg tttttaatat ggtccacgat
    12901 gttggggcgg cgcgcctcgt tgactttgta ggcgcgttcg cattcaatgt cgtgctggag
    12961 caggtgaatc atcgtgccgc gcgtggcgca cagcgtgaat cccatgtccg ccagctgccg
    13021 ggccatgtga agcacccgtt ccttgtcccg gtcattcacg gagatgaaga ctttgccttc
    13081 cgtcggcagg ggattgaatg cactgacctg ggattttgcg aaagcgatat ccggatcagg
    13141 atcaatgccc atcacctctc cggtggattt catttccggc cccagcacca cgtcgatgcc
    13201 cgggaagcgg ccccatggga aaacggcttc cttcacgcaa tagtattccg gtgtgatttg
    13261 ccgggtagag cgcagttgcg ccagcgtttt gccggccatg acctgtgcgg ccagcttggc
    13321 gaggggcttg ccgatggatt tggagacgaa cggaatggtg cgggaggcgc gcggattcac
    13381 ctcaatgacg taaagctgtt catcttttac ggcaaactgg atgttcatca gcccgcagac
    13441 gttcagtttg cttgccagct ccttggaagc acgcgtgatt tccttgtgca ttttcatgga
    13501 aattcccatg gcgggaatca tgctggcgga atcccctgaa tggatgcctg ccggttccac
    13561 atgctgcatg atggctccga tgacggcgcg ctccctgtcc gcgatgacat ccacgtcaat
    13621 ctccacggca ttttccagga agcggtcaat cagaacgggg cgttcctcgg aagcttccac
    13681 ggcttcattc atgtaccggc gcagttcttt ttcctcatat acgatgatca tgccgcgtcc
    13741 gcccagcacg aaggaggggc gcagcagcac cgggtaaccg atgcgcgcgg caatggcggc
    13801 ggcgtcctcc acattggtgg ctgttcctgc ctccgcctgt ttcagcccca gttctttcaa
    13861 cagggcggaa aaatgttcgc ggtcttccgc caggtcaatg gccctggggc tggtgccgat
    13921 aataggcacg ccgtgggctt ccagcgcatt ggccagattc agcggcgtct gtcctccgaa
    13981 ctggacgatg acgccgtccg gcttctcctg ctcgcagata tggagtacgt cttccagcgt
    14041 caggggttcg aagaaaagct tgtcggagga atcatagtcc gtggagacgg tttccgggtt
    14101 ggagttcacg atgatggttt cgtagcccat ttcccgcaga gccatggagg cgtgcacgca
    14161 gcagtagtcg aactcaatcc cctggccaat gcggtttggg ccgccgccca ggatgaggat
    14221 tttttttctg cctgtttcgc gggcttcatt ttcatctcca taggtggaat aaaagtaggg
    14281 ggtgtaggct tcgaattccg cggcgcaggt atccaccagc cggtatccgg gcacaatgcc
    14341 ccgttcctgg cgttccttcc ggatgttttc ttccgtggtt ccccgggcca gggcgatctg
    14401 cctgtcggaa aagcccagct tcttggcttc ccgcagatcc agcttgtcca ggctgtctcc
    14461 cgccttggcc aggtcctgca tttgtgccag gaaccaggga tcaatctggg taagctgctg
    14521 cacttcatcc agggaaaatc cgttgctgaa cgccgtttga atccagaaaa tgcgttctgc
    14581 cgtgggaaca gccagtttcc gcgtgatttc ttctgcggaa ggcgcctgcg gggccttcgc
    14641 gtcaaatccc cagccccagc gtcccgtttc cagggaacgc aaggcctttt gcagggattc
    14701 cttgaaagta cgaccgatgg ccatggcctc ccctacggat ttcatggatg tggtcaggtg
    14761 ttcgtccgcc tgcttgaact tttcaaacgt gaaacgggga accttggtaa ccacatagtc
    14821 gatggtgggc tcaaagcagg cgggcgtttc ccgcgtgatg tcattccgca attcgtccag
    14881 cgtatagccc acggccagtt tggcggccag tttggcaatg gggaaccctg tggccttgga
    14941 agccagcgcg gaagagcggg aaacgcgggg gttcatttca atgacaatca tgcggccgtt
    15001 ggccggatta atggcgaact ggatgttgga accgcccgtt tccaccccga tctcccggat
    15061 gacggcaaaa gaagcgtccc tcatggcctg gtattctcgg tccgtgagtg tctggatggg
    15121 ggctacggtg atggaatccc cggtatgcac gcccatgggg tcgatgttct caatggagca
    15181 aatgacgacg caattgtccg cgcggtccct cattacctcc atttcaaact ctttccagcc
    15241 gatcagggac tcttcaatca ggatttcgga aacgggggaa agatccagcc cccgtgcgca
    15301 tatctcttca aattccgttt tgttgtacgc tacgccgcct cccatgccgc ccagcgtaaa
    15361 ggcggggcgg acaatcaggg gaaaggtgcc aatatccctg gccacccggc gcgcttcctc
    15421 catggtatgc gcaatgccgg aacgcggcac gtccaggcca atgcggagca tggcttcctt
    15481 gaacaggttg cggtcctccc cgcggtcaat ggcgtctgcg ttggcgccaa tcatacgcac
    15541 cttttccctt tccagcacgc cgctgcggtg cagttccatg gcgcagttca gggctgtctg
    15601 cccgcccagg gtcgggagga gggcatccgg tttttcccgg atgatgattt tctccacgaa
    15661 ctccggagta atcggttcaa tataagtgcg cggagctgtc tccgggtccg tcatgatggt
    15721 ggcgggattg gagttgacca gcactacttc ataaccctct tcccggaggg ctttgcaggc
    15781 ctgggtgccg gagtagtcga attcgcagcc ctggccgatg acgatcgggc cggacccgat
    15841 cacgagaatc tttttgatgg aggtgtcttt aggcataatg gcgatggaag aatcctgtag
    15901 tcctggaccg cggatttccg ccgggggaat taagtgcccg ccgggctccg gaggaaagtt
    15961 tttttacggt caatgacgcg gatttttaga gcgtggggaa cggtggaaaa cggaagagga
    16021 aggaggccgg aacaaggcgc gccaaggctc cggagccgtg ccttccctgc gggcagaggc
    16081 ctgacccgcg aggggggacg gcacaactcc ggagctcgtc ggtgcaccca ctaatagatg
    16141 aacagcattt cccggtactt ggggagaggc caggaccggt cgttcaccag ggtttctgcg
    16201 gcatcgacgc tggttctcaa tcgggacatc gcggcaagga tgtcctcatg gcagctcgta
    16261 agcgccgtct ccagggtatc caggtcggat acgatggaat ccagggcgtc gcccagcttg
    16321 tccgccagct tctgcagggc ggcggtaccg ctggtccgcc tggcttccag cgccctggag
    16381 agcgcggcaa cggtttgccc gtattcgttg cgcagggcgg ggaggatgat ggtggccgcc
    16441 atttcccagg acaggcgccc ttcaatgcgg atgcgtctgt ggtattcttc caggaaaatc
    16501 tcaaacctgg attccagctc ccgcctggaa aagacgccgt acttttcaa
//
LOCUS       DAWEMX010000048        16512 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1465_length_16512_cov_12.011667, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000048 DAWEMX010000000
VERSION     DAWEMX010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16512
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_16512_cov_12.011667"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1296..1310
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3862 a         5455 c         4245 g         2935 t
ORIGIN      
        1 ctggtaaaaa ccgtgccaag agaacactac cgggtggaaa aatgggaagc ctatcccttc
       61 ccgggagccg ccgtaccgtt cctggtgctc ctgccggaca acgcttcaga caaaaatccg
      121 gttcccgtgc tattctgcat tcccgggtca gaccagacca aggaagagct ggcgggggaa
      181 acatcaccgg atctggacca gccttccgta cagcagccgg gcaacaacgc catggctttc
      241 cattatgtca ggcagggatg ggcggccatt gtggtggaca atgccggaac gggtgaagaa
      301 ggagacgcgg aacacgccgc aggccgttct tcacacgatt atgaaaacct ggcccgcttc
      361 ctgctggaaa tggactggag ctggctgggc tacacctcct acgcggacca atgtattctg
      421 gattgggtga aaacacgccc ctgggcgcaa aaaaaccata ttatcctcag cggcttctcc
      481 ctgggaacgg aacccatgat ggttctgggc agcctgaacc cggatatctt tgccttcgtg
      541 tataacgact ttctgtgccg cacgctggaa cgcgccaaaa ccatgaccat gcccaacggc
      601 aggggcgtgc gttccgctcc caactccatc cgccacctca ttccgggttt ctggaaacaa
      661 ttcgacttcc cggacatcgt ggcggctctc gctccgcgcc ctgtcatttg cacggaaggc
      721 ggcctggaca gggacttcca gctcatctcc gcagcctacc gtatggccgg ggcgccggat
      781 catttccagt accatcacca gccccggttt gcggaccctg cccaacgctg gcacggcagc
      841 caattgcccg caggtctgga cagggacgcc tttttccgtt tcgccaatgt ggacccgcgc
      901 aaccatttct tcaaaaaaga cctggtcatt ccctggctta aaaccctcct ggataaaagc
      961 gataataaaa actagcccag ctccagacaa aaaccgcccg gaaccgccgg aacaggacat
     1021 tccctcatcc ttagggatgc gaatctatcc ggcggcacat cttccggaat gcatgcccca
     1081 gctttccgca aaagcccgtc accgcccggg cgcccctttc cacgttcatg agcttaaata
     1141 aataacgccg cacggagtcc accaaggcgc atccggcata aaccacggcg gctattgcca
     1201 acacgttcac aaccatcagg gctgaggaag aagacgctgc ccactcaaag acatcccaca
     1261 aaaaccattt tcctatcaaa gcattacaat gcagcnnnnn nnnnnnnnnn taaacgctga
     1321 acgacgctgc cgccagccat gaaatacagg agacagccct tcgggatgaa aaatgcattc
     1381 gggaaaaaat cagcaacagg gcaaccgccg tccctacaat aaaaggagaa ttatagcgaa
     1441 taaacattcc accgcccttg atctgcccaa tcaggtatgg agacaccaca ttaagaacca
     1501 tcttgaaaac ccaggcaaaa catacgcatc cggcataaaa catccataac ggacgcgttt
     1561 tcattcccgt cagcaagcca tgctttttaa catacatccc cagcatgtaa aggatgccca
     1621 gccaccagat gctataccct tcttccaccg ggaacacatc cgtgtgaagc agggtgggaa
     1681 taacggaaaa cagcacgaaa acggtaacca gaagatactg gaactgtaat ttggtcagcc
     1741 tgtttcccag ggcgttaaga aacggaatga agaaaaacag ccccacatac gccgtcacat
     1801 accaatactg gccggataaa acgggaaaaa gggaatgcag aatattactc aactgaacct
     1861 cctgggggaa aaacaacaga gccagcagca aactgcccca tgtataaaaa acaacctgaa
     1921 accataagga aaccacacgt tcatacctga acgttccgtc gcagcacaca tatcctgtaa
     1981 ttaacgcata acaattcaca ccgcaataag cggcaatctc cagcagccac ccgacattat
     2041 aggaagcgaa cccatggttc tccaaagaca aaacatcaat ggaggccaaa agatgaagaa
     2101 ttaaaaccat catcatggcg acaatgcgca aggcatctat tccataattc cgggacgggg
     2161 aaatatattc cggacaacca caggaaaaac gctttccttt ctcttcatca tcagccagcg
     2221 gcaaactcat ggagaaacat ttctacggat ttattatccg cgttctcccc tgctgatgat
     2281 atatttccaa gaaaatcatt tttttaataa aaaagatagc ctgctatgat tttgaaaaca
     2341 ggaaatcttg aaaaacaagg tttttccggt cttttggaaa gtgtgtaaac atggacagat
     2401 tcctctttcc gtgtttggaa ttccatgttt tcaacgggaa ccggatgtcc ctggtgtatc
     2461 aaattttttc ttaaattatt cttcttcaat attaaggctt gaacgcggat aataaatccg
     2521 ctgtccatta ttaaacaatc ctgctgtcca gatcgaacac ctggccggaa gccgccgtaa
     2581 gaacatcctg taaaaacagg ataaacgccg cagcctgttc cggcgtattc aaccggttca
     2641 aaacatgccg ggacaaagcc gcctctttca cgctatcccg cagcacggcc gtcatctccg
     2701 tcagcatgaa ccccggcaac accagattca cgcgaatccc atcctttccc cattcccgcg
     2761 ccaggctttt caccaggccc gccagagcag ccttggaagc ggcataagcc gcctgggaca
     2821 gagccggacg gagcgacgca taagaaccga tcatcaccac ctgcccctcc ctcttctccc
     2881 gcaccatggc ggcggcggcg catcggccgc accaggcggc tcccgtcaca ttcacattca
     2941 ttacctcgtc ccactcctcc tccgtctgct tcaggaacgg cctgtccctg gtaattccgg
     3001 cggcacaaac cgccaaatca tacgcggcat ggcaatcaaa ccagcggcgt acggcggaac
     3061 ggtctcccac atccagtgcg gcatggctcg gggcatccgt ctcccagccc gcagcggaaa
     3121 aacattccac gatggcccgc gccaaaccgc cgtgaccgcc gctgatgaac actctccgca
     3181 tcaaaaaagt ttcctcaacg ttaaaacctc acttggggtc cacccaccgc ccatactcct
     3241 tgatcagatc caccagccgt tccacagcct cctcctgggg aatattgaac ttcacaggcg
     3301 tgtgcttcac atacaggttg atcttgttcg gcgcgccgcc cacataaccg aaatccgcgt
     3361 ccgccatctc gccggggcca ttcacaatac atcccatcac ggcaatcttc acccctttca
     3421 gatgttccgt ggctttccgg atgcgggccg tcgcctcctg gatattgtac agggtacgcc
     3481 cgcaggacgg gcaggaaacg taatccgccc gcgtcagcct cgttcccgta gcctgcaaaa
     3541 tattgaatcc caggaaggaa gccaaacggg ggtccgactc gccctgaatc agcacggcgt
     3601 ctccaatccc gtcgcacagc agggagccga tattgcgggc agccgtcagc ggcacgtctt
     3661 ccccggaaac ggaaccgggc accagcgtat ccttcagcag gatcagatgc ctgtcctcaa
     3721 tacgggcagc cagaagacgg aacgcatata caggctccat agccagcccg ttcttcacag
     3781 taacaataga cggttccgcc agctccttca acggctccag atcggcatcg caccggggat
     3841 ccacctcaat ggcatccagc tgccccaggg ataattcagg catcatgtcc cccatcgcct
     3901 cccgttccgt ctgcagggcg taaaacccgg cgtcaggaac cgccacgcgc acaggctgtt
     3961 cccagcccag gcgcacgcca taacacatcg ccagcccacc ctgccttttg gaaaacttca
     4021 ggggatcata gctcaactcc ggttcaggga aggaaaccgg gtcatcggaa cgctccgacg
     4081 gctggaaagg attggacagg cggtaagcca cgggcacctc cctcacagcg tcctccgtca
     4141 gggaaacgcg cagggtatcc ccaatgccgt ccgtcagcag ggagccgatg cccacggcgc
     4201 tcttgatgcg gccgtcctct ccgcccccgg cttccgtcac ccccagatga atgggataat
     4261 tccaatccgg ccccagggcg ttcatgcgct ccaccagcaa tcggtaagcg gcaaccatca
     4321 ccttgacgtt ggaagccttc atggaaaaca ccagggaatg gtaatccagg tcgcgggcaa
     4381 tctgggcaaa ctcaatcgcg ctttccacca tcccctccgg cgtatcgcca aagcggttca
     4441 gaatgcggtc ggacagggag ccatggttgg aaccgatgcg catcgcgcgg ccatgctccc
     4501 ggcaaaacag aaccaggggc gtaaattctt ccctcaggcg gtccagctcc tctcggtatt
     4561 cggcgtcgga atactcccgc acttcaaact tcttcttatc aacgaaattg cccggattaa
     4621 tacgaatttt ctccacccat ttggcagcct ccatcgcggc atccggcttg aagtggatat
     4681 cggccaccag aggcacatgg cagccggcag cgcgcaattc ccgggcaata ttctccaaat
     4741 tggcggcata cgccttggtc tgggcggtca ggcggataat ctcgcaccct gcctccgcca
     4801 gctccaaagc ctccttcacg caggcatccg tatcccgcgt atcggacgtc agcatggact
     4861 ggatccggat gggattggat ccgcccaccc ccacattccc caccatgact tcacgggtta
     4921 cgcggcgcgt atatcgataa ggactcggac aataggaaga ctgcatgaaa ccactctacc
     4981 gcgccagatt tccctgtcaa ccatccttca aaacggaaag ccttttcccg ccaatccggc
     5041 caccggcaaa acggaaacgg ctacgccccg cggatattgt tccacgccag catcccctcg
     5101 cacgcagtcc ctggcctctt tccccgcctt ccgggtattc cgtcacagca cggactgcac
     5161 gataaacaac acgattgacc ccatataaac agccgccagc accacggcgg cgcagcactg
     5221 aaacagcctg tatccgcagg ccagaacaat cctcctgagc gtggattccg gatgccgccg
     5281 cgcccaccat gcatacagca ggaagacggc ggccgcgccg gaggaaaaca gcagggaacc
     5341 tttcaataaa ggcggataat aatcaaacac cacccggttt tgccctttca gaaggcgcac
     5401 cgccatcaat tcccccacct tttccacagg cgcctccatc ccgttccgcc gggctctcca
     5461 tccccggtca tacacgatcg gcaccatgag agcctcccct tctccggcat tcagcgccac
     5521 ctccacatgg ccaccgcttc ctttcgcctc catcccgtac ttatcctgct ctttccccag
     5581 aaccgggaca tcctgctccg gcgtacgcgc cgcagccgcc aaaaggggcg tctccacggt
     5641 ccgcagcatc ctcccctcca ccacggtttc cccggcaacg gccgcacgct tcaattccaa
     5701 aaccccgtta taggtgcggc tcgttcctcc ggatgtcttc tgcgcctccg ccatgacggg
     5761 caccgcacat ccattgacac ggagttccgc cgtatccgtc tgcgggaacc cgtacaccgc
     5821 gccgggatat tccggaatgt ccgcgcggta aaaggggccg ctcatctccg tccttactgt
     5881 tacggccgca taaagggggc cttccgccga tgggcagagc gcggcgtgca gggcattaag
     5941 attgctgaac acatcagcgt tcccatccag acgcagcccc agcgcgccct ggggaaccac
     6001 cagcccccag cgggcgcccg gaagcctgta taaatacatg ccttcccggg aaagcacgca
     6061 ctccattccc tccatgggcc gggacgccag cacaaaaccg tggcccagca gcaaatcgga
     6121 aaacagagtg cctccctggc cgtacgtgcg cgtgaattca tcccggtatc ccaggttttt
     6181 cagaaaacgg aaatgcccga tgctcgtcgt atgccggaaa ttgccaatgc ccgggacagg
     6241 ccccaggcaa tccgcgtttt ccaccatcag gcgatccctg tccttcacgc gcagcaagcc
     6301 tgaatacccc ttcatgccgc gcgccagcca ttccgcattg agcgcctgga cggaggcttt
     6361 ctcctcctcc ccctgcttcc agaacgcggc agcccctatg ggcagcccca gcatcatcac
     6421 ggaagcgatg gaccacgcca gccttttgcc ctccacatgc ctccaacaca aacatgcgca
     6481 tgcaaaaaac agcaaaggca gaagacgcac cgcaagatcc gcagccgtca tcttcaggac
     6541 aaacagggaa atgaaaaaag ccgctatggg aagccccagg ctccatcccc acccccatgg
     6601 ccgtcctgac ggccccggca gcctgccgcc ggacaaaatc caggcgcaga acgacattac
     6661 cgccagattg ggaataaaac cgaaccggac gggaaaatcc acgtagctcc ataaatgggc
     6721 ggcgcggtgg atgggttcga caaacgcggg caaaacggac gccgccagaa gaaggataaa
     6781 caggcgcctc cctctgtccg gcgcagggga cgccttcttc ccgcaagcca gataaaccgc
     6841 cgccagagcg gcgaatgaca tccccggcag gcaggcgtaa aggcgttcca ggaatttggg
     6901 agggtcaaac ggccatgaca attcaaaaaa cgtgggcggc gtcataaaca gggagcgcat
     6961 ggaatcccgg ctcatttccg ccatcggcag ccagtaataa caagtggcca gcgttaccgc
     7021 cgccgtgtac cagaacacgc gggccaggcg cacgcggcgt tcccgcgtcc ccttcagggg
     7081 ctgcgtaaag gcccacgcga acagccagcc ggtgacgacg gccatgaaag cgctgtacag
     7141 ggaggtagcc agcaggaaga ggccggcggc atacagccag ccccttcccc cgttcaccag
     7201 ccggtaaagg cccagggcaa acagggggaa catggcggca tggtccagga attgcatgaa
     7261 cccgtacttg gtctggaaaa agccccccac ggcataaccc gccgtcagca gcacggtcca
     7321 gcatacgggc agggagggaa acacgcgtct caggaaccag gaagccgtca cgcagatgca
     7381 ggccacatcc gccagcatca cccagaccat gtccttcaac aggaaggaat cccccatcgc
     7441 cacggcaatc caggtggagg ggatcagcag ctgatgcggc agcgtagggc tcacccggat
     7501 tcccgcgccg ccattgacat cataaaaaaa agctccgtca aacgctccgg acatcagggc
     7561 ggaggcataa tacttcaagg taggataaac cagctgcccg ttatccatat actccaggaa
     7621 gcggcctccg aaaggccaga tcccccgtac cgccagaaaa aggaacacca ccgccaggga
     7681 aaatgcaatg gacgccaggg acgtcaatac cggacgggaa gaagcggatt ccatggctca
     7741 atcagcggac aaacggttca gcggcccgga tcagccggag ggcgcgggcg cattccgcca
     7801 cgtcatggac acggtgaatc tgcgcgccgt gcaaatgccc ccacaccgtc atcacggcgg
     7861 tgctcagcgg cgcgcgtccc tgctccgcat ccccaaggac ggcgcccagc atgcgcttgc
     7921 gggaaaggcc cagcagcacc gggcggcctg ccacctgaag cttgtccatg ttggacagca
     7981 gggccagatt gtgttccgcc gttttgccaa agccgattcc gggatcccag cagatctgct
     8041 ccggttccag cccccgcccc agcagaaaat cataacgttc ctcaaaatag ccgcgcactt
     8101 cccgcaccac atccccataa gaaggagcgt cctgcatggt ttcaggccgt ccctgcatgt
     8161 gcatcgccac cacgccgcag cgcgccgcag cgcacaattc cgccatcccc gcctcccgca
     8221 atccggaaat atcattaata acgtccgcac ccgcttccag cacagccgcc gctacggcgg
     8281 catggcgcgt gtccacggaa atcacggcat ccgtgcatga acggagttcc cgcaccacag
     8341 gcagcaccct gtccaactcc tcctccgcgg aaacctccgc ggcccccggc ctggtggact
     8401 cccccccaat gtccagaata agggctccct gccgttcaag ctcttttgca tgggagacag
     8461 cgcgttccgt cgcataaaaa cgccctccgt cggaaaagga atccggagta acgttcacaa
     8521 ttcccatcag cacgcctccg cgcgccacat ccaatacgtc tctccttaca agccagttca
     8581 tctaattacg ttcttgtcaa agccactata catcacttta ataagtcaga aactcttttt
     8641 tcaccatgaa aacaacgccc ctgcttctct tcatccccct gctcatgacg tccggagccc
     8701 tggccgccgg ccaggacgat ttcaatgtcc agaccgcttc ttcccgcatg gtcaaacatg
     8761 aagacggttc ccgcagctac tttgaaaaaa caggggacgg aaaagggatg aaaaaaacca
     8821 cttacaacat ccgcaatgtc ctcgtttccg taaccctgta caaaagaggg ccttttggag
     8881 agctgcgctc ctgcctgatc tttgacgggc agaaaaacga actctttttc gtgcgttacg
     8941 gctacgacgg caacgccaac ctgagagaag agcaaatgtt cgattcccgc accaaggaac
     9001 tcgtgcgccg cttcctttac acttatgacg ccatgggcaa ccgcagcaag cccgtatgca
     9061 tcacgctggt caagagtacc aaggacattg aaacgcacgc cgtgcccacc gccccggaaa
     9121 aagacccctt cgcggacgat tttaaaaaga aaaacaaata agctccggtc cttcgggacg
     9181 attcctcccc ttccctcctt ctccgtcttt cctcgggacg ccgaatcttt atcccccgtc
     9241 cggtccggcg cacgctccgc gcaggaaaat tgcggacggt cctgaaaggg agggctgcgt
     9301 tcccgccctt aaaaaagccg gacccaggga cattcccgga accgggccgc ggcaacaaca
     9361 catcggtcgg ttccaatccg gcgcggcatg gtctgttcaa agaagaggca ggaacagttt
     9421 tctgaaagtc ttcctattct ccatcgcggg cttccagcct gttccgccag cggcgggcca
     9481 cccagggccg caccagcccg taaagcagat aaatggaaaa aatgactgcg ggcatcaccc
     9541 acgggaattt gaaaatgcag atgaccgcca gcacgatgaa cacaatcgcg tacatcgtcc
     9601 cccgcgtgcg catgttgata tgcttgaagc tgggatagac cacccggctc atcatcagaa
     9661 tggagactcc cgccattgcc agcgcaatca cgtatttgaa gacgcccagg tccatggccc
     9721 cgtccggagc cctgcccgcc agatacatca ccaggtacat ggtggagacc acggctcccg
     9781 ccgccatcgg aacgggcaga cccacaaaat cgctgctctg gccttccttc cggggagcgg
     9841 ccgccatgca attaaaccgc gccaggcgca gcgcggcgca cagcaggtac aggatgccga
     9901 tgccccaacc cacctccgga ggggacagct ggaacaggac ggccttggcc accagcagcg
     9961 ccggggcgat gccgaaggag acaatatccg ccagagaatc gaattcccgc ccgaacgggc
    10021 cgtcctgccc gcgcatgcgg gcaatacggc cgtcaaacaa gtcaaacaaa catgccgcga
    10081 agattaaaaa agtggcattc tgataatagg aaaaagcagc cacagcgtcg ctgtccgcct
    10141 ggttgattcc ctcaaaaatc gtcagaatgg cgaaaaaacc gcacaccaaa ttccctgccg
    10201 taaacaaatt aggcaaaatg ggaatctcag gttcgtccgg gaaaatcttc ttatccatga
    10261 ttggtgtccg atagggccca ggtaaggggt tatccgttaa taatattgcg ggcggcggca
    10321 gccagggcct gaggatcggc atcctgcgga gcggaaccgc gggcctgatc cgcacggccg
    10381 cctcccttgc ctccggcaag agcggcaacc tcgcggatca tatctccggc ggacaatccg
    10441 tccgcaatgg catctttgcc acaataagcg cccaggagca aggaagaact gtccacgcac
    10501 agcaggaagg cggcgcccgc atactggcgc tttttcaacc cgttcagcag ctcctgaagc
    10561 aattccccgg cgccctccgc cacctggatc agggaagagg gggcatcgga aagccactca
    10621 ttcagcaggg cgtctgcctt ggcggcggac tgcccggcgc gggctttttt aagcttcttc
    10681 tccgcatcca gagccgtctg tttgaaatgc tccacggagg cgtcgaaacg cgccagggaa
    10741 tcatttaccg tccggatatc ggaagccccc agggccgtca gccccggctt gccttcaatc
    10801 gtggggacag gcacctgttc cagccccatg tccgccaact cgtaattgac ttccttgatc
    10861 ttctccaccg ccttggcgat ttccaggctc ttggcaacaa catgctgccg tatcatttcc
    10921 agagcagcgt ccccagtcat cgcctcaatg cggcgcacgc cggaagcgat ggcaccctcg
    10981 ctcttaatct tgaacaggcc gatattcttc gtattggcaa tatgagttcc gccgcagaat
    11041 tccatggaaa ccccgtccag cccatccctg catccgccca cctggaccac gcgcaccaca
    11101 tccccgtact tgtcgccgaa gaactgggca atcgcggcat tgcccttcac gtccgcatag
    11161 gcgcgttccg tgcagtgcac gggaagagac tcctcaatcc agccgttcac tttctcttca
    11221 atcaggcgga gctgatccgg agaaacggcg ctgctgttaa agtcaaagcg cagccggtct
    11281 tccgaaacaa aggacccctg ctgggccgca tccgggctga ccacctgatg cagggcgcag
    11341 tgaagaagat gcgtggcggt gtgatgcgcc tcaatgcggc ggcggcgttc cgcgtcaatg
    11401 ctcaaatgca cgcggtcgcc gggcctcacc tccagcccgg gacgggcctc caccacatgg
    11461 gctcgggcat tccctatctg ctggacggcc atcacatggt agctttcccc gccgatttca
    11521 atcaaaccgg catcggacac ctgcccgccc atttccgcgt aaaacggagt cttgtccgtg
    11581 atgatgaaca gggaatcccc ctggcggctc acttccagca ccgtagcggc gcattcgtcc
    11641 acatcgtatc ccgtaaactc cgtcacggcg tccgtcttca aatccagggc gcgcaccacc
    11701 tcgctcttgc gggcggcccg ggcgcgttcc cgctgttcct ccatcagcct gttgaaccgc
    11761 tccatatcaa tatccaggcc gcgttcctcc gccagcaggg cggtcaggtc aatggggaac
    11821 ccgtacgtat catacagctt gaaggcgaac tcgccgctca cctttccggc ggaagccgtt
    11881 tccgcgtcaa acaattccag gccgcggtcc agcgtctcat taaaactggc ctcttcacgg
    11941 ttcaaaacct ccttcacggt agcggcgcgg gcggccagtt ccgggaacac ctgtccgaag
    12001 gactccacca gcgtatccac cagttccgcc aaaaacggct gggtaaagcc caggcggcgc
    12061 ccatagcgca cggcacggcg cagaatgcgg cgcagcacgt aattacggcc attgttgccc
    12121 ggcagaatgc cgtccgcaat ggaaaaactg agcgtgcgca gatgatcggc aatcacgcgg
    12181 aaggcaatcg cctcctgaag ggtcccgtcc tccgcatcca cccttttgga accgggcgcc
    12241 ggatacacgt ccgcgtactt ccgtccgctc aaaacttcca ggcggtcaaa cagggggcgg
    12301 aatacatccg tggcgtaatt ggacggtttg cgggaaaaat ccttgaatcc gttcgtgcac
    12361 tgcatgatgg agcacgcgcg ctcaaacccc atgccggtat ccacatgaca tgccggaaga
    12421 ttgcgcatgg agccgtcgct ctccgcattg tactggataa acaccaggtt ccatatctct
    12481 atgcactggt cggaatcctt gtttaccagg cttcccttcg tattcccctc cggggtcagg
    12541 tccacgtgca gctcggaaca ggggccgcag gggccggttt cccccatcat ccagaaatta
    12601 tccttcacat tcccgtgcac gatatgcacg tccgggtcca gccctcggga acggaacagc
    12661 tcagcccaga aatcccaggc ttcccggtca aactctccgg ggtcgccctt gctcttgtcc
    12721 ggcgcgtaca cggtggcgta caggcgttcc gccggaaatc cccaccgctc cacgaccagc
    12781 tcccaggccc agcggatagc ttccttcttg aaataatccc cgaaggacca gttccccagc
    12841 atttcaaaaa acgtgtggtg gtaggagtca taccccacat cctccaggtc attgtgcttg
    12901 ccgcctgcgc ggatgcactt ctgggtatcc gtagcgcggg cgggcgtcca cgggggagtc
    12961 catacgccca ggaaatacgg gacaaactga ttcatgccgg catttgtaaa caacaaaccg
    13021 gggctctggg gcatcaaaga agcggaaggc acgaccgtgt gctgtttttc gcggaaaaag
    13081 tccagaaagc tttggcgtat ctcggtggcg gtcatcataa cagataatgg gaaaagtaaa
    13141 agacgccgga ttatgcctgt tctccctccg ttagtaaatc gctaattcct tctccgtcat
    13201 gcgggctcca tttttacttg caataaatta tacacgcgta taaataaaat tatgaaaaag
    13261 ctggttatcc tcctggttct ccttgcggcg gcaggaacgg ccgcctggct catctaccgg
    13321 aaaactccgt ccgggcccga ggacaaagcc gttctttacg gcaatgtgga cctgcgccag
    13381 gtggatctgg ctttcctcat ctccgaacgt attgactccg tgctggtgga cgaaggggac
    13441 acggttgttc ccggccagaa gctcgccacg ctggaaacgg tgcggctccg gcaggccgcg
    13501 gacgaagccc ggcagatagc ggaagccgcc cgccaaaact acctgcgcgt taaaaacggc
    13561 ccgcgcgcgg aagaaatagc ccaggcgagg gcgaacgtgc aggccgcgga agctacgctg
    13621 aacaacgccg gaatgcgcag caaaaggctg gaggcgctgg ctgaaacaaa atccatctcc
    13681 cgccaggagg cggacgacgc cgtcgcctcc cggcaagtgg ccgccgccaa tctggacgta
    13741 gccagaaaac agctggaact gctgctggcc ggttcccgtg aggaagacgt agcccaggcg
    13801 ctggcccagt acaaccaggc caaagccagc ctgaccatca gggagcagaa cctgaaagac
    13861 gccgtgctct atgcccccgg caacggcgtg gtgcgcaacc gcattctgga aaagggggac
    13921 atggcctccc cgcagaaacc cgtttacaac atctccctga accataccaa atgggtgcgg
    13981 gcctacctca cggaatccca gctcggcaaa gtaaagcccg gcttttccgc caccgtgcgc
    14041 aatgacagct tcccggacac cggcttcaaa gggacggtgg ggtttatctc ctccgtagcg
    14101 gaattcaccc ctaaaaacgt ggaaacgccg gatctcagaa cggcactggt ctatgaagtg
    14161 cgcatcattg tggacgatcc ggacaaccgg ctgcgcctgg gagcccccgc caccgtcacc
    14221 atccccctgg accagaacgc cggaacacag cccccggaac agcccaggcc atgaccacgg
    14281 aatcccagcc cctgattgac tgccggaacg tccggaaaat atttccggac tccgccggcg
    14341 tcccctttgc ggcggtagac gacgtctcct tccgcctgtc cgccggggaa atcgtagggt
    14401 tgctggggcc ggacggagcg ggaaaaacca ccctgatccg cctcatcacg ggattgatga
    14461 aaccgcatgg cgggtctatc tccgtcctga atctggactc cgtgaaaaaa agccggtcca
    14521 tccaggcctc cataggctac atgccgcaga aattcgggct ttatgaagac cttaccgtcc
    14581 gggaaaacat ggagctttac gcccgcatgc acggcgttta cggccaggac agggaaaagc
    14641 gcttccgcag cctgctcgcc atgaccagcc tggaacgctt caccacgcgg cttgcgggaa
    14701 agctctccgg cggcatgaag cagaagctgg gcctgtgctg ttccctggtc tcctccccgc
    14761 ccctcatcct gctggatgaa cccaccgtgg gcgtggaccc gctctcccgg cgggaactgt
    14821 ggagcatcct gaaacaattc tccggagagg aaggcgtggg ggtgctggtc agcacctcct
    14881 acatggacga atcggcctac tgcaacagaa ccctcatcat gtatgaaggg cgcctgctta
    14941 tggacgcccc tcccgccgac gtcatcgccc gggcggaagg catgtgcgtg acggtgcgca
    15001 cgccagaggg gctgcatgcg cgccaattcc agagcaggct ggccgccgtg cccggcatca
    15061 tcaacgccac cccgcagggg aacaccgtcc gcatcatcgt tccccacggc caccccgcac
    15121 ggaaaaagct ggaggaatac catcccgtcc ccgcgcagcc ggacttctcc gacggtttca
    15181 tgaccctgct tgccggccaa gtggacctcg ccccgcagga catccccgcc cctgccggaa
    15241 cgccggaacc ggagcggacg gaaaacggcg gcaccgtcat ccgggtaacg gacctcgtca
    15301 ggaaattcgg cagtttcatt gccgtcaacc acgtcagctt ctccgtccgc aaagggcagg
    15361 tcttcggcct tctgggcccc aacggagcgg ggaaaagcac cactttccgc atgctctgcg
    15421 ggctgctgcc cgccagcggc ggcacgctca atgtggccgg ggcggatctg cggaccgccg
    15481 ccgccagggc gcgcaggaaa gtgggatacg tggcccagaa attttccatg tacggcatgc
    15541 tgaccacgcg tcaaaacctg gaattcttcg ccggggccta cggcatgtcc ggaaaggagc
    15601 ggcaggaagc catccgctcc atggaagaag aattccacct gactccgtac atgaacgccc
    15661 ccgccgcgca cctgcccggc ggctacaagc agcgcctcag catggcgtgc gccctgctcc
    15721 actccccgga catccttttt ctggacgaac ccacttccgg agcggacccc ctggcccggc
    15781 gcgacttctg gctgcgcatc aacgccctgg cggaaaaagg cgtcaccatc atcatcacga
    15841 cacacttcct gggagaagcg gaattttgcg ataacatgct catcatgatg gatgggacca
    15901 cgctggcgga aggttccccg gacgatatcc gcaaacacgc tcccccgcgt gaagacggag
    15961 cccccgcttc tctggaagac gccttcctgg ccatcacgga agaacacatg aaaaaaggag
    16021 gagaaacatg accgcctccc tcaaaagaat aggagccctc atcgtcaagg aactccacca
    16081 ggtggtccgg gaccccggca atgtgggcat tgcggtcatc cttccggccg tgctcctgct
    16141 cctcttcggc tacggcatga gcatggacat caaaaacgtg cgcatcgcgt acctggccgt
    16201 ccccgcctcc agccagtcca cggacctgga aacaaggctt accctttcca gatatttcca
    16261 gaccacgcgc gtcttctcta cccgggaggc tgaagaaaaa ctgcgcaccc atgaagcgga
    16321 cgccttcatc gccctgcaaa gcaacgcgcc ggacacgctc cacaatggaa ccacgaaagt
    16381 ccagattgtc gtcaacggag tcaacgccaa ccaggccacc ctcatccgca actaccttca
    16441 ggccgtcgtc ggctcctggg cgtcttccct gggcgggcgg acagcccccg tgctggacgt
    16501 gcagacgcgc ac
//
LOCUS       DAWEMX010000049        15799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1550_length_15799_cov_11.803100, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000049 DAWEMX010000000
VERSION     DAWEMX010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_15799_cov_11.803100"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9043..9052
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14959..14968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3475 a         4086 c         4632 g         3586 t
ORIGIN      
        1 tccactttaa gaatggtttc aacggatgcc tggctggggg aaacccccgc tgcattgacg
       61 agcatggaaa tattgccgta cctctgtgct tctgcaatca aatgcaggat ggagtcccgg
      121 gaagagagat ccatctctaa ggggatggtg tcaaatccgg cctgattcat cgtccgggca
      181 atggcatgcg cgttcccgat atttttgtcg ccaatgacga ttttcatgcc gcttcctatt
      241 ctgcgggcaa tggccatgcc gatttgcccg gctcccgtca atatcatgac ttttttggat
      301 agacgttgtt ccatgggcta tgaaatcagt tgaaatgacc ggtttttatt cctagccata
      361 tgttatgttc ctctagccgg acggtatggc cctcccgcaa aaagggcaat gcaaaaacgg
      421 ccataggtga tggtattgca aaatgggtca tatggattga aaaactgagg gggggatgaa
      481 agaacccagg caaaagtcat ggcagcatgc tgattctttt cagccatgtt ttgacgcctg
      541 cggcattgcc cggcatgtca atcccctcat catcggcacg tccgggagtt gtaatatctc
      601 ctgggtcaag gtcttctgga agagttgacg gaatgtgccg ggaatggggc gtaagcctgt
      661 aaatttgttg catggattcg tttaaataag tagtcgcgtc ataagtaaag aagggaatga
      721 gcgttttccc tttcaggtca aatgcctcta aaaaggtgca aatcaccatg gcaggctgat
      781 gccaccagat tggagagccg acgaagatag tatcatattt cgccatcagg gaggcttcag
      841 gcaagttggc gacctttggg cgcaggtttt cataagcttc ttttttgaca cggtcgctgt
      901 cgcggtaagg gtcggcactg taggcctcgg cggcttcaat ccggtagata tccgctccgg
      961 tcagtttcgc aatttgttcc gccaccgctt tggtattgcc cgttgcggag aaataaacaa
     1021 ccagcggttt tcccgcatct tgtccggttc tgcggacaac aggcagccct tttgcctgca
     1081 acagcgtgtt cagctctttc cggactgcgt ctgcctcccc ggggccgatg ttccgggaca
     1141 caatatcaag cagctggtgc agctgtgcgg gtgttatgcc cagattcagg cagatgccca
     1201 tgtgagagcg ggccattggt tccacaccgc ctatggccgc gaggatggat acggtagcca
     1261 attcgcgttc atcataggtc agaacgtccc gttcaaaaat atctgcaaaa aggtgttctt
     1321 tcaggaaacg ttccatgacc ggggcaaatt cggcataggc agctttcggc gcattgacgg
     1381 gaatgccgga gatttccgca agaatggcgc acccgcgatc gtagcggctg cgtttgtcct
     1441 ttgtggggca ggcatcttgt cccggggcat cggctatgcc tcgggacttt cttttatcca
     1501 gcacggagat gaatgtctgc aatcccctca ggcttcgcgg aaacccgcaa taggcgtagg
     1561 aatgcactaa aacttcccgg atttcattta tcgtcaatcc actgtccaag gcggcattca
     1621 gggccggctg cagctgatgc aaatcccctt tgccggtata ggcggcaatc gtgatgatgt
     1681 gtttctgcct ggaggtcaat tcctggctgg aaagggcgga atttgccgcc agagccagta
     1741 cgcacgacat caggcagaac aggacagacg ataaaagttt catgaacctg ggagagcagt
     1801 tttacaggtt tttcccgaag aattccgcca gctttccgac agcctgatcc acgtattccg
     1861 gcacgtagta ggtcttgata tgcgtggcgc ccggaatgag gaacaattcc ttgttgaggg
     1921 ttcccgtggc gcggctgtag cattgttccg tcatgtagaa ggtgtcggct ctgctgcccg
     1981 ccatcatcag caggggctgg ttaatcaggt gcataccttc gctcgcgtca aaggcaaaga
     2041 ggtccatcag gctgcttttg gtatagcgga aggtggaatt ggggtgggcg tggctttgca
     2101 gataatagtc gtagccgtcc cggtagaggt caaagggcag ttgggccgcc tgttccggcg
     2161 ttactccttt catgtctccc acgtattccg gctgaccgcc ttccgcttcg cgctggcggg
     2221 cggcgcaggc gtcggcaagg cgttcctgca cgctgttgat ctgcgagtcg agaaacccgt
     2281 tgcgccggac caggccggtg ttgaacatgc tgagcgtggc gactgccttg aaacgcttgt
     2341 ccgtctgtgt ggttttcagg gtgtagcctc ctccgccgca aatgcccagg atgcccagac
     2401 gggaggcgtc cgctccggga tattgcagca ggatgtctgc ggcgcggcgg atgtcttcca
     2461 tgcggttggc gggtttgtcc gtattgcggg gagtccctcc gctggccccc tggtaggccg
     2521 cgtcaaatgc caggcagatg tagccctgtt ccgccatgcg ctggctgtaa agtccggcca
     2581 cctgctcctt cacgccgccg ttgggatgcg ccacgacaag agcggggtat tttttcgagg
     2641 catcatatcc ggcgggcgta taaacatggg cggcaatttg cagtccgttc agcgtatagg
     2701 tgattggatg gatgttgacg cggccttcca tgttttccgt gacggctccc cggtaaacca
     2761 gcccccaggg attggcgttg tggcttttaa tgctttttgc ttccgtaaat tgggattggc
     2821 gggctggggc tgttgaaacg accggagaat catttgtgtt catttgctgc gcatttaact
     2881 ggtggttgaa ggaaatcagg gaaagggcta ttgaaataat ggcgtttgtt ttcatggcat
     2941 gtactttgct ttctcaaaca gttcccattc tatcatgaaa ggctctcttc ccgtcgatag
     3001 acggattaca ggttttgcta ccatgattac aggttcccgg aatgaactgg atagccggct
     3061 cccttcctgt tatgctccgg tcaaaagcag agaaagggca tgggcattat tctgaacatg
     3121 gattccgtag gggactacaa ctcctttttg gggcaggaaa cgctgcaccc tctggtcagc
     3181 gtcgttgatt tttccagggt cccgccgctg cggcatgtcc gcaaggctta cgggttctat
     3241 gccgttttcc tgaaggacct gaaatgcgga gacctgcggt acggaaggca ttattatgat
     3301 taccaggagg gaactcttgt cttcgtatct cccggccagg tgctgggcaa tgatgacaac
     3361 ggggaatatt tccgggtaga gggccatgcc ctcctgttcc atcccgattt gctccgcggc
     3421 acctccctgg gacgcaggat gaaggactat accttttttt cctatgacgc ttccgagtcc
     3481 ctccatatgt cggagaggga aaggaattta ttcctgaacg gcctgacgga gatccgggag
     3541 gaactggaac ggggcgttga ccggcatacc aagtccatcg tgacggccaa tatcgaagtt
     3601 ctcctgaacc actgcatgcg tttttacgac cgccagttca ttacccgcga gaatgtgaac
     3661 agggatgtgc tgacggagtt cgaacgcctg ctggattcct acttcgaggg agacaagcct
     3721 caggaatggg gcctgcccta tgtgcagtat ttcgcggatg aactgcatct ctccgccaac
     3781 tatttcggtg atctcgtcaa gaaagagacg ggaaaaactc cccaggagca tatccatatc
     3841 aagctggtgg acaaagccaa ggagctcctt tgcgccacca ataaatccgt tagtgaaatc
     3901 gcctatgaac tggggttcaa gtatccccat cacctgagcc gcatgttcaa gaagaatacg
     3961 ggatattccc cgaacgagtt caggttgtcg gcgtgacgcc cgttccctgt cgccggacgg
     4021 cctgcggcat tctgtccgga gcggaacggt tcgttccgga ttgattgccg ggccgggaat
     4081 acagaaaata atcttctgtt ccgaacggca ataaacagga atgcatcttt tttcagctgt
     4141 tctgttgtcc gcggtattcc tattggcgca tgcgggcagt ttccggcgcc gggggaatgt
     4201 attgccgggg ggtacaataa tggcttcagc ctgccggatt catattacgg ttttctgccg
     4261 tggagaagaa ataacgtcat cccggaaagg caacctttta tgaacaggta ttttatgaac
     4321 gtgtggagcg attgtgtgcc attactgccg tctttggcct tgttacggtt gcggacaaag
     4381 ggtatggtgc ggcaagataa ccgcgatatt ggacggcatg atgaatgaaa gcggtctgcc
     4441 gcccgccagg agacgaccgt ttgcatcctt cccgcaacgc cgttccgctg aataatataa
     4501 aacactacaa taatgggaaa ctcaatagac catggcatgt tccggaagtc cgtcctgctg
     4561 gctttcggat tgtccatttc cttctcgggc ttcgcgcttg ccgcatcgga agaaccggtt
     4621 ccggagcaac aggcccaggc cgttgaaggc gccagaaaaa tcaatcctgc cgccgtggaa
     4681 gtgttgcttg acaacaaccg ccggatgacg ctcgattttt acggagacaa tgttttccgg
     4741 attttccggg atgacagagg cggtattatc cgagatccca aggcggaacc ggaagcccgc
     4801 attctggcgg acagtccccg gaggccggtt tcccggctgg atgtggacca gcagggcgat
     4861 gcggttgtca tcactacggg gaaggtcagg attgagataa ataaaaagac ctctcttttc
     4921 aaggtgatca atctgaagga taattccgtg gtggtggagc aggcgtcccc cgtccttttt
     4981 ggaaagggca aaaccagctt ttccctgaag gcgaagccgg acgaatattt ttacggcggc
     5041 ggcgtacaga acggccgttt ttcccacaag ggaaaggtca tttccataga aaaccagaac
     5101 agctggacgg atggcggagt ggcttccccc actccctttt actggtccac cggcgggtac
     5161 ggcgtcatgt ggcatacctt caagaaaggg cagtatgatt tcggctccag ggaagagaat
     5221 ctggtgaacc tctcgcatga tgaaaattat ctggatgtct ttttcatggt cagcgacggg
     5281 ccggtcagcc ttttgaggga tttctaccag cttaccggcg ctcccgttct gctgcccaag
     5341 ttcgcctttt accagggcca tctgaacgcc tataaccggg attactggaa ggaagatgaa
     5401 aaaggcatcc tgtttgagga cgggaaacgg tacaaggaaa gccagaagga taacggaggc
     5461 attaaggaat ccctgaacgg ggaattgaat aattaccagt tttccggccg cgccgtcgtg
     5521 gaccgttaca aggcccatga catgccgctg ggatggcttc tgccgaacga cggctacgga
     5581 gccgggtacg gccagacgga taccctggac ggcaatattg cgaatttgaa gagcctggcg
     5641 gactacgcca ggaaaaacgg cgtggaaatc ggtttgtgga cccagtccga cctgcatccc
     5701 aagccggaaa tcagcgcttt gctccagcgc gatatcgtga aggaagtgcg ggacgccgga
     5761 gtgcgcgtgc tgaagacgga cgtcgcctgg gtaggcgcgg gctattcctt cggtctgaac
     5821 ggtattacgg acgtggccca gatcatgact tactacggga ataacagccg cccgttcatc
     5881 atttccctgg acggctgggc cggaacccag cggtatgccg gcatttggtc gggcgaccag
     5941 acgggcggcg tctgggagta tatccgtttc catattccca cctacatcgg ctccggcctt
     6001 tccgggcagc ccaatatctg ttcggacatg gacggcattt tcggcggaaa gaacccgttg
     6061 gtgaacgtcc gcgatttcca gtggaaaacg ttcaccccca tggaactgaa catggacggc
     6121 tggggagcca atgagaagta tccccacgcc ttcggggaac cttacacctc catcaaccga
     6181 tggtacctca agctcaagtc ggaactgctt ccgtacgctt acagcattgc ggaggaatca
     6241 gtttccggcc tgcccatgat ccgggccatg ttcctggaat atcccaatcc ctacacgctg
     6301 gggaaagcga cgcagtacca gttcctctac ggcccttact tcctggtggc tcccgtttac
     6361 caggaaacca gggcggataa ggaggggaat gacgtccgcc atggcattta cctgccggaa
     6421 gggcagtgga ttgattattt caccggggat ttgtatgagg gcggcaagat ttacaatgat
     6481 gttgacgctc ctttgtggaa gttgcccgtg ttcgttaaaa acggcgcgat catccctatg
     6541 gcgaacccga gcaacaatgt ctcggaaatc aatcccaatc tgcgcattta cgaactttat
     6601 ccgcacggca gcacctcctt taccacgtat gacgacgatg gcgtgacgga ggaatacaga
     6661 accggcaggg gcgtccgcac tctggtggag tccagggtgg acggcaaaaa caacgtgacc
     6721 gtcactgttc atccggcggt gggggatttc gacggtttcc agaaaaagaa ggccacggaa
     6781 ttccgcatca atgtgacgca gaagccgtcc ggggtttccg ccaaaatcgg ggaaaacagc
     6841 atcaacctgg cggaagcgaa ttccctggag gatttcaaat ccagggagaa tgtttatttt
     6901 tatgaccggg ctcccaacct gaacaggttc gcaacgaaag gcagcgattt tgaaaaaaag
     6961 gtgatcgccg ggaatccgca attgctggtg aagctggccg cagcagatat cacggcggcc
     7021 cctacggtgc tttccgtgga agggttccgg tttgaaccgg ctgaaaagta tcgcctttct
     7081 tccggagccc tgaccgctcc cgccaatgct gcggtgacgg aggaaaatgc ggcggcctat
     7141 accctgaagc ccacgtggga cgccgtcccg aacgccgatt tttatgaaat cgagtttgga
     7201 ggaatgctgt acaccaccat caagggaacg gaattcctgt ttgaggattt ggaggccgag
     7261 actccttatt ccttcaaggt gcgggccgcc aaccgggacg gccattccgc gtggacggcc
     7321 gtcagcgcga agacgaaagc caatccgctt gagttcgcca ttccgggaat cgaaggtgaa
     7381 acgagcgtgg aaaaccaggg cagctccctg gcgaagctgt ttgatttcaa ggaaaaggat
     7441 gtgtggcaca cgaagcatga tgcgaaggct gttccgtttg acctggtcat ggacctgaaa
     7501 acgattaaca ggcttgagaa gtttcattac gttccccgcg aggacggcgg caacggaacg
     7561 ctgctgaaag gcgctgttta ttacagcatg gaccgggaga actggacgaa ggcaggaacg
     7621 ttccagtggg ataaaaatgg agacgtgaag atattcggct tcaaggatgc tcccacggcg
     7681 cgctatatca agcttcatgt cacggaaagc gcgggtgatt acggctccgg cagggaaatc
     7741 tacgttttca aggttccggg tacggaaagc tatttgccgg gcgatatcaa caacgacggc
     7801 aagattgacc ggaatgacct gacttcctac atcaactata cgggcctgcg gaaaggcgat
     7861 tccgactttg aaggctacat cagtaacggg gacatcaaca agaatggcct tattgacgct
     7921 tacgatattt ccgtggtagc cacccagctg gaagacgaag ccgaccagcc gcaggaagaa
     7981 gccgacaaga aagatgagga agaggataag gcgggagggg acgacgagaa gaaaaaatcc
     8041 gccgaggagg caaagaagaa agtccgcgtg gacggaacgc ttttgcttag tgcggataag
     8101 aaaaggtatt ccaggggaga tgccgtcaag gtgtccgtga agggccggaa cctccggctg
     8161 gtgaatgccc tgagcttcgc tcttccgtat gaccccaagg atttggaatt cgtgggcgtg
     8221 gaggtgaaga acatgaagaa catggagaac ctgacgaacg acaggcttca tacgaacggg
     8281 accaaggccc tgtaccccac cttcgtcaac atcggagaca aagagcctgt ggaaggaacc
     8341 tccgaactgt ttgtgctgaa attcaaggct cgtcgcgata tgaagttcca gccgaagctc
     8401 acggacggca tggtggtgga taaaaagctg aacaccaaaa aattgtaatt taacatcctg
     8461 atacggctcc atgcccggcc aaatggcggc cgcgcatgga gcctgccgtt ccggcggagt
     8521 atttccgcag tattcatcgc tgtggagcgg tgttcttccg catgcttcgg aggttttcgg
     8581 acggagcggc gacgcggaaa tattttcctg cctcgcgttt ccggcggagc gtgacgcaat
     8641 gaggctcttt ttacaggcgg atgaaccgtt tttttctggc gcgaccggtc ttttccctta
     8701 tgttgggtac gccagacagg ccccacgatg ccatgaccgg ccccggactc atctccttgt
     8761 tctgcttttt aatgacggcc gcctattttt tgcgcggcgg gatgtgtgcg ggcgccgttt
     8821 tgtgcatggg cctggcgttt ttttcatttt cccgtcgcgg ctggcttcgc aggagcgtca
     8881 cgtttctgct gcaggcttcc ctgcttttct ggggggcgga agcctggcgt ctggccaggc
     8941 tgtggatgat ggaagggggg cctttcctgc tctggacttc cattccggct gctgcgttgt
     9001 tgcttcatgc ggcggccatt ctatggcgga ggcgcggaga aannnnnnnn nntgcccgtt
     9061 ccggagctgg cccgcagccg cgtattttcc gtttccgtat tgcttctgtt tttgctggat
     9121 gcgctggttc ctttccgttt gctgatgggg gagcgcatct tgccgtggca gggggctaat
     9181 gggctggcga ttcttctcct ggcctggtgg ggcggctatt gcgcggaagg tttgttgaat
     9241 ccgcagacgt ctccgcgcag gcggcaggtg atgtggaccg tgtttgcctc cgcttttttt
     9301 ctccagtttc tgctgggagt gacggtggct tcctccctgc tgatgacggg gaagctccat
     9361 attcccgtgc cgtttatgat gatttccggc ccggtttacc gggaagaagg gtttttcatg
     9421 ctggcattgt tcagcgtatc cgtccttatg gccgggagtt cctggtgcag ccatttgtgc
     9481 tacttcgggg tgtgggactg cctggccgcc gcttcttccc gcaggaaggg gcatccggtg
     9541 ccgggtggga aaaaagcctg tgactggagg tggttttccc ttgccgcggc ggtggggatt
     9601 cccctccttt tgtccgtgtg gggcgttccg ctggggtatg ccctggcggc ggcgttcgcc
     9661 gtagctttca cggctccatt tgcctggaag aaaagttccg aaaacggtgt gcgggaatat
     9721 tgcagcaggt tctgccccat ggggctgacc gccagtttgc tgggcaggct gtccccgtgg
     9781 cgcatgcgcg tcagggagac atgcaccggg tgcatgcggt gcgcctccgc ctgccgtgat
     9841 ctctccatct cgcgcggggg cggggcctgc cgcatttccc ggcgctgcac cttgtgccgc
     9901 gactgtattt cccggtgtcc gcatggcgct ttgagcctcg gcatggccgg ccccttttcc
     9961 tctgtgccgt ccgtgcgggc ggatatgtat tttgtaacgc tggtttccgt catgcatgtg
    10021 gtcttcctgg ccacggctcg aatctgattt ttatgttgcc atgaaagatt cctgctccct
    10081 ggagtatccg ttttgcggag gatggatgga atctgtccat taggaggctt tttcagggtt
    10141 gccgtcagag ggattcaggc agcggtgcag gagggggacg gccctgaatt catggaacgg
    10201 catggttccc tcttcccatc cgaaagagtg gattttggtt taagaaggcc atttttgtta
    10261 ttcattgaga gatttattga tgatttaaag tttttgttat gatttttgaa tgaccgttca
    10321 atttattaaa gtcactaaaa aagatagggt ctgctatgct ccggtgccgg agccgttgga
    10381 agagggggat aagctggaaa tcatggaaat gaaggatgaa gcccttttcc cgccggcagg
    10441 gaaaaacgca aggatttccc tatccaggaa tcccatggta ttcgtgaaaa tgaatgacgg
    10501 attccagcgc atcctgatga agaatatcct gtatgtggag gcgggcggaa gttattgcac
    10561 gctgcacgtg cacggcatgc cggctatcct ggtatccgct ccgctggcgc gggtatccga
    10621 gcatctggat ggggaatatt ttatccgcgt gcaccgttcc tacctggtta acaagctgca
    10681 tatagaatcc attgcgggca acttcctcca ggtgggcgtc gttgccattc ccatcagcaa
    10741 attcatgaag aaaaaagtgc tgggcagttt gaacatgctg tcgttttcca aataagttcc
    10801 ggcctgccgc acgggctcct gcggcgttcc gggaaagcga aatggaacgg ccggaaaggc
    10861 gggatggacg cagaaacgga atggccgcat cattcacgac gtcgccggaa ggcggcatat
    10921 caatgaatct aaaccagaaa tagaaattaa atatatggca attgacagat cagcagcaga
    10981 agcggcgact tcctccctgc agtcgctgcc tttcggcaac atcatcggcg gtcccctggt
    11041 cgcctgtgtg gaagcacagg cccaggcggc ccggacttcc tgggaattca tccagaacgt
    11101 gggcctgtac gcggatgggg aagagaagaa gacggtgaac gtttccttcc agttcatcaa
    11161 ggacggccgc atggctcaga ttacggttcc ccttctgacc atcgtcccca ttccctatat
    11221 cgccattaac tccatcgaca tcaatttcaa ggcgaatatc agcgcgtccg ccgccagtac
    11281 ggagacggaa aattcctcca gttccgtaga caccagcgtt tccgcctctg caaggtgctt
    11341 ttgggcgcgc ggcagcatga atgccagcta ttccagcaaa aaggactcct ctgcgaccag
    11401 ggattccaaa tacagcgtgg aatacaccat ggacgtagcc gtccatgccg gacaggacag
    11461 tatgcctgcc gggatggcca aggtgctgga gatgctgaac aatagcattt ccgtcgtttc
    11521 tccggaaggg ttcctggacg tgcagcatgc catccaggac gacggaaccg tgaaattgat
    11581 aacctcctac aaaaacaggg aagggttgtt tgacccggaa gccatcaccc tgcggatagg
    11641 cgaagcgacg gaaatatttg aaaacggtgc ggggaatgaa gccaggattg tgaaaacgga
    11701 ttccggcaag gaatacacac tttccaggga agacgccaga aactgcacgg tgagcgctgg
    11761 agaactcaag aggaaggtgg ctgtaggcgc ctgataaccg aaccatagcg atttctcctc
    11821 cattccagag ccatgtcccg tcttaatctc atcgtggaat ccctgctgaa ggacattatt
    11881 gaagcgcagc atgcggcaaa ctgccacgcg gcatcactgg cgcgccagta tgggaagaga
    11941 ggccgtgcgt gcggtttccc gctgcccagc gcccagatag gctccctgga attcgatttg
    12001 aaatatgcgg tgacgggaac ttccggggtg gaggagaaac aggttgcgga cacggagcag
    12061 tggagcctgt tccggcagga actggaagag cgcgtgccgg cgtttgccat ccgttccatt
    12121 gtgatggccg tggtgcattc cagccttccg gacgacgaag cgagaagcga cttctcaagc
    12181 ctgctggcca gggaggaaca gtggaaaaag aatttctgct cctacctggg gaagaaaatt
    12241 tccgcagccg tttccgctca tgagggcgcg ctcctgaagg cagaacccgg gctggacagg
    12301 ggattgctgc tggagcttct ggcaggcgtc gcggaaaggg aattcatgga taatccggag
    12361 ctggaggacc tgttcctggg aacccgggga gaccccctca ggcaggaatg ccgcgccaat
    12421 ctccgggaag gcctttcctc tttcttggcc gctctggcgg aaaagtacgt cctcaccagg
    12481 caggaaagca gtgtggtggt ggatgtggaa gtagaggcgg acaagctgaa aaagatgccg
    12541 gaggaagccg tgcagaccct gcgcctgaat atatcccctg cttccattcc ctccgttctg
    12601 gcggagcaga atgatgtttc cgggttgtcc gcgcaatagt gatagatgat gaatgattct
    12661 acaaataata acaaggtgaa cgggcacatt atgagcctgc agcagctgat ttcagcgccg
    12721 ctggtcgcaa cgattgacgc ggatgcgatg tccacggaac gctacatgaa gcatttcatg
    12781 tccctggcct ttgagtccta caatcccgtg gacggttcca ccggggcatt gaggttgatc
    12841 agcttcaatt ttacggacag cgacgcttcc ggcggcgcgg aaaagaaggt cagcgtgccg
    12901 ctcgtcagcc tggtgacgct gccgctgctg caaatcaaac aggcggattt tgattttgac
    12961 atcaatatca ttgacgccgt agccgccgcg ccgaatgaac atttttccct gaacagggga
    13021 gaggtggagt cccccggcgg aaaagagcgg gatgaaaggc tgaattttcg ggcttccctg
    13081 gctccgcagt ccgggagagg ggcttcttcc tccctgcagg cgaacatgaa aatccatgtc
    13141 tccatgcagc aggctgacat gccttccggc ctgtcccaat ttctccagct ggcttccagg
    13201 ccgcattatg aggatgtccc ttcccgggaa aagaatgatt aattgaaaga aacgatatgg
    13261 accagaaaac aacttattcc taccagcgta ctccgggcct tgactgcccc aaatgcgggg
    13321 tgtatttccc cacgaccatt ccggacttgc tgtccggctc catcaggtgc ccttattgcg
    13381 gcctgacgct ttacattgac cggaaagaat caggccatgc catgcaggcc ctggaaaatt
    13441 tccagaacgc attagacaag cagcttccct ccgcctccct gtcatgaaga aaccgttgat
    13501 tatcctgtcc gccgtgctgt gcatggttct gccgtccttg tctccggcgg agatgcggca
    13561 gcgggaaccc cgccagcttg ccgccgcctg gcaggaagta aagaagcaga tagcagacga
    13621 cgggaaaacc atccgtgtga gggacggcat tcaagcgctt gaactcctgg cggaactggg
    13681 gatctcttcc gttccggcgg acggcgtcag gctgtcccgc ggcagtctga ccattgtggt
    13741 cacgcatggg aagaaagagg acggggtttt ttacgtgcta gctgttaagg aagaaggggg
    13801 cgcaagcctg gaagtgaaga tggttcctcc ttcctgattt gccggagagg aaggtcattc
    13861 attttcaaaa tcccacacgc ggcggcgggc gtttttcttc gtccgcccgc cgttggcttt
    13921 ccggaagggg tcaaaaagag agtccagccc gttccctttc agcagtaggc actgttccat
    13981 cagacggccc agtttccctt tcgggaaccc tttggcctgg aaccaggcca gatattcctg
    14041 ggggacgtcc atcagcggac atcccttggg cgggtatttg gcaggaccgt acatgccgaa
    14101 agggatatgg gtctgcgcga tttcctccac cagttttctc aaatcttccg catctggtat
    14161 ttcaggatcg ttcataggtt ttgcagaata aatggttgca ggcattccgc cagcctttcc
    14221 ggcgtttcca ggggaagccg gtggccggca tgagggatga tgacggcttg ccctcctgcc
    14281 atgcgccgcg ccagggcgct gaatctggca tcgcgctgtc cggcaatcca cagctgggga
    14341 acaggggatt gcttcaaaag cggggccagg ttttcctgtg cgcccacgga ccagtcaata
    14401 aaggcgcggg agatggattt gcgccacggt ttcagggaag aacggtccgg aggcggcgga
    14461 ctgccttcaa atacgccctg ggcatcccat tccttcaaaa aattttccca agatgcggaa
    14521 aggcattgga cggcccattc cgcatccttc gcccgccgcg ccgcccgttc ctcttcatgt
    14581 tccaggccgg gattggcgct gacgaatatg gctcctttcc agagaggggg atgggccaaa
    14641 acggcctgca tggccaacct gccccccagg gaatagccgc agatgagggg atgacgatcc
    14701 tgggaggcta tttcggaaca aagcacgcgc cccatatcct tcaggctttt ggggcagcat
    14761 tccagatacc tccagaggtt cagggcgcga gcctccactc ctccggcacg aagaaaatcc
    14821 atgacgggtt tccaatccgc cggagaaccc agattgccgt gcagaagcca aagcatggtc
    14881 ggaaccgtct ttctatttga cgccgtagcc ggcgaggccg ttcataaaca tctggacgga
    14941 aatcatgatg agcaccatnn nnnnnnnnaa gaccaccatg cccatcatcc gttccaaggc
    15001 gattgtccct ttttccccca gcagacgcag gaacttctgg gcgatgaaaa gcaccattcc
    15061 ggagaggaac caggcggtca tgatggcccc ggcataggtc atctgggaaa tgctgtccgg
    15121 agtctgggag gcgtgcagca taatgatgga cagggaagag gggccggcca tcaggggcat
    15181 ggcgatggga acaatgaagg gttcgtcttt tccgtccgct cgtccggaag aagaaagcat
    15241 atccttggcg gggaagacca tgcccagcgc tacgagaaac agcaggatgc ctccggaaat
    15301 gctcagggtg gaaggttcca gccccagcag gttcagcagg aattttccgg aaaagaagaa
    15361 aaggagcagc aggctcaacg caaatacaag ctcccgtgtc agaatagcct tctgctgggc
    15421 gggcgagtat tttttcagca ttccctggat catgggcgcc agacccaccg ggtcgatgac
    15481 gatgaacaga agaataacag tggaaaggaa gtcctgcatg ggatatgccc cgttgtaaac
    15541 gatacgggcg tgtacgtcaa agggaaccgc cagcagtgga aattttctct gcccggattg
    15601 ctattttctt caccagcgca ggagccgggc ctttatgcct ggaaagcgat ttgaggaatt
    15661 ctggggaaac tttgcgtagg agaccatagc tttttaataa aacagaaaag ctattagcgc
    15721 aagaatcctt tgggcgaccg gcgtgacaaa ctaaattctg ccttccctac atacaccctc
    15781 cgagcatcgg gatacgaac
//
LOCUS       DAWEMX010000050        15669 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1568_length_15669_cov_13.458435, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000050 DAWEMX010000000
VERSION     DAWEMX010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15669)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_15669_cov_13.458435"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3596 a         4404 c         4064 g         3605 t
ORIGIN      
        1 gctgacgaca cgccccttca ctccgccccc cgtttcccca tccgcgtttt ttccgatatt
       61 tcccccatca tgaaaaccgg cccaataaaa aaacccggct tccttgcgga aagccgggta
      121 tgggaaaaat gcctcaggga agaagttttg ccgggcgccc ttcaaaaatc ccgcgctgag
      181 gaacaaaaat gcggtcattg ggctggatgg gaatcgccct gtccttaatg gaaaaataat
      241 catacgtcac ggtttttcca ccgcgcgtca cctgcacgta gcgggatccc cagtccgtga
      301 tgtcgccggc gcccacaatc gcctccagaa gcgtcatgcc gcgccgccac aaaacattct
      361 tcttcatagc cacaccgccg cttaccagaa cgatgtgttc cgtcagccgc gcatccgaac
      421 tctgcacctt caccagaaac gtaggggccg tgtacaactc cgccttcttg taggcggcca
      481 tcagcatgtc ttcaatctgg cgcgccgttt tgcctaccac gcgcagcaca tcctgccccg
      541 gagggagctg gggcatgcgt atcgttccgt tcccgtccaa caaatactgc ccggaaatag
      601 cggtagcgtc gtcggcggga atgccgcgca cctggatttc aatggtgccc tggggcggca
      661 cggcatccgg agactgggcg gagcccagcc ccgccgggcc cagcataaga caaaggatgg
      721 aacaaatata ttttttcatg gagcaatggg ttaatacaaa tcttcactat catgggactg
      781 ggtggctgcc agctccttgg cctttccaga acgttccacc ttcttcatgg aagaggcatc
      841 cgcgttctct ccggaggaag cgccatccgc agaataatag gaatagtaag ttgtgtaata
      901 ctgatattgg tgatcggacg taatgtccac attattcatc accacgccgg ccaggttgcc
      961 gcccacggac tcgattacct gcttctgcct caacagggct ttcagcggca gcttgcgggg
     1021 ctggacaacc ataagagtca tgtccacctc gcttacaatg acggcggagt cactgacccc
     1081 caggatggga ggggaatcca ccagcacaat gtcaaaacgt tgcttcactt cctgcagcaa
     1141 ttcattaaac ttgcgggaat tcaaggcgcc cgaagggtca aagggagtgg gtcccgcagg
     1201 catgacgtac aggttttcaa tgggcgtctg aaaaatgact tcctccagag gataatcttc
     1261 cagcaggtag gaagtcagcc ctacctcatt atccagctca taataccggt gaagcttgga
     1321 acggcgcagg tcggcgtcaa tcatcagggt ggcatacccc ccctgggcac aaatataggc
     1381 cagattgcac agggtggtgg tcttcccttc cccggcgctc ccggaaacaa aggtcaggga
     1441 aatttcttcc agccctttct tattgaattc tatgttcgtc cgcaaaatgc ggtaggcttc
     1501 cgcatccggc cccatgacat cggaagaatg cagaataggc acattcttgg gaataatgcc
     1561 caggacggga acctgaagag cccgttccac atcttccatg gtcttcacgg aggtatccat
     1621 gaattccaga agcaaggcca atccgatgcc cagcaacaga ccggcacccg ctcccaccgc
     1681 caggttaatg gtcacattcg gcttgtaggg cgcagcagga taggtcggca gctgatacat
     1741 ttcaattgcc tcacggggta cgcgcaaacg ggcaatctca tctttgtacc gggaatccag
     1801 ttccttaagc tggtccagcg cagcgtcata atcacgactg gcctgttcat acatgggaac
     1861 ctgcaggacg tgcttctgaa gatcctcttc cttttgggca acggtcgcct cccagtgctt
     1921 ccggcttgcg gcaaccattt ccagcttgct cttcagggaa gaacgcaggc ctatcaattc
     1981 cttgttcagc ttatccccca gaatcctttc cgtttctagc agcgccagca ttttgggatg
     2041 cctggcgccg atattctggg atttcagatt ttccttttcc ttgagcttgt cggaaaattc
     2101 agcataggtc ttacggagag cgtccgcccc tacagtctcc gcattcagca ggtcggaact
     2161 gatcacgtaa tccagcaact tgtcgtcggg cagacgctgc aattcctgca cataggaagc
     2221 cagttccttt tccctccttt caaaatccat ggcctgcgct ttggcattcc ggaattcctc
     2281 ttcttccata ccggccgtca gggtgctgcg gtgttcaccg tttctccagg acgtaggcag
     2341 gtaggagctc tccgcaatca gctttttgag agccatcatt ttttcattga cggcatcctc
     2401 ctgttcccgg aggacatcct tcaggctctt gatggcggtt tccaccagac ggttttcgcg
     2461 catttcacgg tcctgcttgt acgcctcagg caccgccttg gcaatattct gcaccagttt
     2521 gggatccgga ccggaaacaa tgacatccac aaggtccgtg gcacgcacgg gggccacctt
     2581 aatcatgccg gccagattag tcgccgccat ttcatccgtc atcccccatt ccgcggcaag
     2641 gttcagtttt ctggatacca ctttaagcac ttcggcggaa gtcagcacgg aatactgcat
     2701 gggaatgtag ttggccgtca cgttggaagt ggcaaccacg tccccccccc tgcctatatc
     2761 catcacaggc ttgggcagtt taatctgaaa ctggctggtc gcccggtagg agggcggcat
     2821 caggaaagtc actaccacgg aaatgaccag caccagaagg aaaacaagaa agacttcctt
     2881 ccagcgattt tttaaaactt gcaaatagtc aatattgcgt acgatgcgat cactgttctg
     2941 ctgatcctgg gcactcatgg aatatagtaa ggcagtaaga taaaaactcc tctaattgtg
     3001 aatgaaaatt cgcaattaga ggagtaaagg cattaatggt cagtcaagac ttagaagctg
     3061 taattcacgc cgaaggagaa cacgttgcgg ttgtaggaag gcatcgggta gttatagccg
     3121 ctgtaggaac cgttcgtgta ggaatattgg gccgtaaggg ccagggcatt cgtcagcaga
     3181 agcttgatac cggcggtagc attgaaagtc aggttggacg tgtcactgtt gctgatggaa
     3241 ctcggacgcg tataatcgct ggcaatcagg tttactccgc aattcagact gacgcggtgc
     3301 gtcagcttca gcgtgctgtg taccccgaaa cgccacgttt cattggagta atagctggcg
     3361 ccgttgtaag ggatgtaggt attcaccgct tcgttggaat aacggacgaa cgttccgagc
     3421 atgaaccgat cgctcaagcg gtggtttaag ccgaattccg cggagggata ggtttttgtt
     3481 ccgtagttct cgacatactt gaactgagga cctacgcgca gcgtagcaga agtattttcg
     3541 ctcaccgcat attcagcgcc ggccatgacg aagttggaaa attcgttatt gccgtattcg
     3601 cggttacagt aggaacccgt ccagcccagg gaaacagcca ggcgttccgt ccatttgtac
     3661 cggttggtga aattcatgcc aacataatcg cggttatccg tcttggcgga atcctcctga
     3721 tagtcgatca tggagaaatt ggcacccagc gtcgtggaat agcgggaagt ccacgccttg
     3781 ctcacggaac tggaaacata cacgtaaatg tagtcgccgt cccggcgggc attggcaata
     3841 ccgttggaat agttgggttc cggctgccat gcaaaggaga cgctgccgtt ataacgcaaa
     3901 gtctggtcaa aactatggga aagtttggcg gacagcgtgg aattggaaag ggcttcattc
     3961 gttccgccat caaggtcttt caggtaaaaa atacccccca gcttggcgga gaaagaatat
     4021 tgggtcacgg catcgtagtt ggccagggag caccctatgt ccgtactgat gaatgcggat
     4081 tcctgcttgt tataattgga ggcgttgaca ttgtcgtcat accctcccgc caggttgaag
     4141 aaccacttga gaggaaggga ctcttccaga ccaaccggac ccgccagcgt gtacagagac
     4201 tgcccgttgg catggacggc aaacatgccg gcggccacca gggagagata aaaaagactc
     4261 ttcatgctat tttattaaat aagaatcagg ttaattgcca gcagcattgg acacgggagc
     4321 ggggctggtg ggcgtgccgc ccacaggaag accgcccggc agaaccaggg agcccccggc
     4381 aacatcttca acaggcttta ccaggccatg ttcctggaca gttacccctg agagggggtt
     4441 atcaggagtc tgggcagcca cggccacagc aatggaagga acagtaccgg ctaccggaga
     4501 attgcccaaa gctttttcaa ccgcttccga aatgggagcc acggatgcat acggatgggg
     4561 ctgggaggaa acagcggaag cgatcacatt gcgcacctgt tcttccttca ccccgggccc
     4621 gtagctccgg acagcggcgg aaacaacggc aggagcgttg gacgggaaag cgcccgcagc
     4681 catggatacc atcgcaccca tcacatgatc ggacacacca gccttagcag catcgttcaa
     4741 aactccggac accgctttca tatccagagc accggctttc aatccggcct gaacaatatc
     4801 tgccattttc ttcataaaag ccttttcaac agcggaggct ttaacctgat caacagccgg
     4861 agcggctgcc ggagaagcag tttgctgagc cataccggga agggtacccg caaacgcaag
     4921 tgccagcgca ctaagcgccg cctttgtcac gaacctggct ttcataaagg aagtatttag
     4981 caggtgaatt taataaaaaa atggagcggg taacgggaat cgaacccgta taatcagctt
     5041 ggaaggctgg agctttacca ttaagctata cccgcgcaac gaacgcatta tgttcaaatc
     5101 acgggataag acaagttttt tttcattact gcgtcaaaat aacaaaaaaa gaggatacgt
     5161 tttttctgtg gtcttgacat ccgggccttc tgccgctaaa taggcatccg catttcatgc
     5221 gggcgtcgtt caatggtagg acgcgagctt cccaagcttg agacgtgggt tcgattccca
     5281 tcgcccgtac caatttgttc cgcaagttat tgtttatcaa agcttgcgtt ttttgttttc
     5341 ttgagacggc aacatacatg gcaacgcctg gaaacggcct ttcaagacat tgtcccacag
     5401 gcagaagaca tggtttatcc gcaaagaagc cttgttccgt gtgaataaaa aagacctggc
     5461 agctactccc aatacgggaa cagtccgggc aagatgtccg cgccggatgc attcgccgcc
     5521 ggcacggacg tgaattacag ggagaggcta ccagccaaga acaaatttcc tctggatttt
     5581 agcaagaaga agcactttgc ggatcattgc ccgggtttca ttaaggaggt tgagatagag
     5641 aagcgcccgt cgcatatcgg aggcgtcttc cggacgcaga ataatctgct gtttgacggc
     5701 ttccgcaaat tcctcatcca gggcgtccat gcctgccagg cattcgtcca acccggtata
     5761 gtcattgctt tccatcattt cccggaaacg ggggtagagg gaggaaacac ggctgttcat
     5821 tccccgcaga agctctatcc tttccaaatc cagcccttca tggttgttgt ctatataggc
     5881 atggctggcc gtagtaatgt tggagaggga ttcaaagacg gaggtagcgt attccaccat
     5941 ctgcacatag cgcttgcctc ggtcggcctc tttgtccggg agttcacgga cgacgggcat
     6001 gacttccatc gccattttat cgctcaggga acggcggatg ccgcgcgctt ctttccggag
     6061 ttttttcaga cgcttcctgt cctccacgtt cagggcctcc agattcattt catagatttc
     6121 cgccatgcgc ttcatcagtt caataatttc ctcattgtag cgtgcggcgg cagccggatc
     6181 ccggtaattg gcggcctgcg cctgagctgc agcctgggtt cttttacgat ggagcctgga
     6241 acttttcaga agcagaaaag ccgccagcga acacatggcg atgatgccga agagaccgcc
     6301 ataaaggatg caggccgcca caatgaagca catggaaaac gcggcaaagg ccgtaaaaaa
     6361 ccaccctgaa atcaccgtaa tcactccggt gatgcgatac acggcgctgt cccgccccca
     6421 ggccttgtcc gccagggagg aacccatcgc caccatgaac gtcacatatg tggtggaaag
     6481 gggaagccga agggacgtgg ccagagaaat cagcagggca gccaccgtca aattcacgga
     6541 agcgcgaatg aggtcgaaag atgcaccgtt atccggccct tccggcacag gccggaagcg
     6601 tttttcaata aacctcccga cgcaggaggg catgattttt tccaccgcgc gggaagcatt
     6661 cagggcatag cgcaccgcca tgcgggccgg ggggacggag ccgaaccgct ctactccccc
     6721 gccctgcctc gccagattga cttccgtatc cgtaacggtt tgcgcctttt tggagaacat
     6781 gagggccagc accatgatga cgcccgcccc cagcaggtaa cgccaatccg ccgtgacggg
     6841 ggccatcaga ccgcccatgg acagagtggt aaggtccgcc ccctgcgctg cggcggcggc
     6901 cgcaatttcc atagaagact gccccgccat gaaaacgccg atgaagttga ccaggtcatt
     6961 cccggcaaag gaaagggcca gggccatagt gcccgcgaga acggatactt tcaggatatt
     7021 gactttgcaa agattctgga gcacggccat caggacggtc cagaatgcca ggctgcagca
     7081 aacgtacagc cagatatggg cgtccaggtg gcggatcata tccttggtga ccagcgtgct
     7141 tcccttcagc cccttgaaaa tggcaaagta agtgatagcc gtcagggcca cgccgcacca
     7201 gacggcgccc aggtatttga aggatttttg atagttgaag gagaagatga ggcgggaaat
     7261 ccacataacc gttatcccgc aaatgaaagc gataaaaacg gaggaaaaaa tgccggagat
     7321 gatcgccaga gctttggagg aattgatgta gctggacaat tcctgagcga cgcccggttc
     7381 cccggaccaa attttaacga gagctgcggc cacggctccg cccagcagtt caaaaacaat
     7441 ggaaaccgtg gtggaagtgg gaaggccgaa cgtgttgaaa acgtccagca ggataacatc
     7501 cgtcaacatg accgccagaa agatcaacat gatgtcatgg aaggaaaacc gggccggcat
     7561 gaatacgccg cttctggcga tttccatcat gccgctggag aacagcgagc cgatgatgat
     7621 acccaaagcg gccaccagca caatcgtcct gcgcggagcg gccttggaac caacggcgga
     7681 gttcaaaaag ttggcggcgt cattggaaac tccgacaacg aggtcgaagc aggataacac
     7741 cagtatcagt cctatgataa agtaatagat cgggtccata tgcgtaataa acggccctct
     7801 attacaggag gccgctcccg cacgcccgtt ttaaaacgtg aacattccgt cacattttcc
     7861 catacgcccc tgcttccggt cctcaaaaaa ggaggagagc ggattcttcc gccctcctcc
     7921 cgctttcatt aaatgtaaaa aacaaaaagc ctaacggtcc acatgaagga tatttccgtc
     7981 aggggcaatc agcacatcat aatcgtcacg gctgtccgca acttccacct tgtaacggac
     8041 aagttctcct tcagtaattt tttccgcttc cttaatccgg catcccggat aacgcttcaa
     8101 aacggacgca cggacggctt ccggaagaga ggaagcagct atttcttcct taatcctcac
     8161 aattttccca tcaggagcaa tcttcacgtc aaactggaat ccgttaattc taaattcggc
     8221 ttcgtagatc ctttccatgc catgcatttc agagtcccat tcaatgggac cggcgttcgg
     8281 ataagcaccc ttgatactgg aaacggcggc agccggaacg gaagactccg ggatatcggc
     8341 cagagcagaa ggcatggcca gtaccgccgc cgcaaacaat atgttcttat gcagcttttt
     8401 catgttatga ttagacaaat atgttctata atcattcaat atttttctaa attctgaaca
     8461 tcctgtgcag ctttttcccg aaaccggctc ctgatgaagc atattaaaaa aggctccccg
     8521 gtttttccgg ggagccttga agccgattgc cttggtctcg aagaaacgga cggggcttac
     8581 atcatgccca tgccgcccat tcctcccatg ccgcccatgt cggaagctcc ggagccgcag
     8641 ctgcagctct tcttctcggg cttgtcggca atgacgcatt ccgtggtaag caacaggccg
     8701 gcgatggagg ccgcattctg cagagcggaa cgggtcacct tggtcggatc caccacgccg
     8761 gcggaaagca ggtcttcata cttgtccgtg gccacgttgt aaccttcggc agtattcttc
     8821 atgcctttca cgttggcgac gatgagggct ccttcgcggc cggcattgca ggccagctgg
     8881 cggagcgggg cttccacagc gcgataaacg atctgggcgc cggttgcttc atcaccttcc
     8941 agtttgaggg tgtcaatggc tttctgggcg cgaatcagcg ccacgccgcc gccgggaacg
     9001 atgccttctt ccaccgcagc gcgggtagcg tgcagggcgt cgtccacacg ggccttcttt
     9061 tccttcattt ccgtttccgt agcggcaccc acgtggatga cggccacgcc gccggccagc
     9121 ttggccaggc gttcctggag tttttcgcgg tcgtagtcgg acgtggtgtc cttgatctgg
     9181 cggcgaatct gggaaatgcg ggcttcaata tcggaagatt tgccggaacc ttccacgata
     9241 acggtttcat ccttggaaac aaccacgcgc ttggcctggc cgaggtcttc gatgcccacg
     9301 ttttctagct tgatgcccag gtcttccgtg atgcacttgc cgccggtaag gatggcgatg
     9361 tcttccatca tggccttgcg gcggtcgccg aagccgggag ccttgaccgc gcagatgttc
     9421 agcacgccgc gcagacggtt gactaccagg gtggcgaggg cttcgccttc aatgtcttcc
     9481 gcgattacca ggaagggacg gccgctcttg gccacttttt caagcagcgg aaggaagtcc
     9541 ttcaggttgt tgattttctt ttcgtggatg aggatgtagg ggttttccag caccgcttcc
     9601 atcgtttccg cgttcgtcac gaagtagggg gacaggtatc ccttgtcaaa ctgcatgcct
     9661 tccaccacgt ccagcgtagt ttcaatgccc ttggcttctt ccacggtgat ggtgccgtcc
     9721 ttgcccacct tgtccatggc ttccgcgata atgttgccga tttcagcgtc ccagttggcg
     9781 gagacggtag cgacctgggc cacttccttg ctggagtcga caggcttgct gatcttcttg
     9841 agttcttcca caacggaatc cgcagccttc atgatgcccc tctggaggga gatggggtta
     9901 gccccggcag taacgttgcg caggccttcg cggtaaatgc tttcagccag cacggtagcg
     9961 gtagtggtgc cgtcgccggc cacatcattg gttttggaag atacttcccg gacaagctgg
    10021 gcgcccatgt tttcaaacgg gtcttccagt tcaatttcct tggccacagt tacgccgtcc
    10081 ttagtgatga ggggggaacc gaattttttg tcaatcacta cgttgcggcc ggcagggccc
    10141 agcgtgctct tgacagcctt ggcaatctgt tccacgccgc ggagcagagc ctggcgggcg
    10201 gtttcgtcaa attggatttg tttagccata atgttaaata tttaaaatga tgttgaatgt
    10261 gtgtgtggtg ccatttagcc gatgatggcc agaatatcgt tttcagacag gataaggtag
    10321 tcttccccgt ccagtttgat ttccgtgccg ccgtacttgg aaataagcac gcggtctccg
    10381 ggcttcacgg taaattcaat cagggcgcct ttttcatcgc ggccgccggt gcctacggag
    10441 atcacttcgg cttcctgggg tttttccttg gcggtgtccg gcaggaacag gccgccggcc
    10501 gtcttggttt cagcttcaat gcgcttgacc agcacacgtt gtcctagggg tttgatgttt
    10561 gccatagtat gttttttgat gttgttgtag atgagtaata cgggatgtaa tgttccgccg
    10621 gagggggaat tgcttccccc tccggcggga agggggttgc ttatttatcg tcgtccacga
    10681 tttcagcgtc caccacgcgg cctttcgcct tgcggggacc gtcggaaggc tcttcttcgg
    10741 caggggcgcc cggcatgggg ccggctgccc cggcggactg ctgggcggct tccgccgcct
    10801 tgtacagggc ttccagacgg gattccaggt cttccttgcc agccttgatc gccgtgacgt
    10861 ccttcttgga gacggcttcc ttgagacgcg ttaccttgtc ttctatttca cgcttgaggt
    10921 ctgcgggcag cttgtccccc atatccttaa gctggcgctc cacggagaag cagagggagt
    10981 cagcctggtt aatggtgtca atttcctcag cgcgtttctt gtcttcttcc gcgtgggctt
    11041 ccgcatcgcg cttggcgcgc tcaatttcat ccttggaaag gccgctggag ccctggatgg
    11101 aaattttctg ctccttgccg gtgcccttgt ccttggcgga tacatgcaga atgccgttgg
    11161 cgtcaatgtc aaaggtcact tcaatctgcg gaacgccgcg cggagcgggg gagatgccgt
    11221 ccagcttgaa gttgccgagg agcttgttat cctcaaacat cttgcgttcg ccctggcaaa
    11281 tgcggatatc cacggcgggc tggttgtcag cggcggtgga gaacacctgg ctcttgcgca
    11341 ccggaatggt cgtgttgcgt tcgatcatcg gagtggcgat gccgcccatc gtttcaatgg
    11401 acaaggtcag aggagtcacg tccagcagaa gcacgtcatt cacgtccccc tgaagcacgc
    11461 cgccctgaat agcggcgccc agggccacca cttcatccgg gttcacgccc ttgtggggtt
    11521 ccttgccggc cagcgtatgg gccatttcct gcacggcggg catgcgggtc atgccgccca
    11581 ccagcaccag ctcgtcaatg tccccggttt tcaacccgga cgcagcaatg cagtccagca
    11641 caggcttgcg ggtgcggtcc agaaggtctt ccgtgagctg ttccagcttg gggcggctca
    11701 gcgtcagttg gatatgcttg ggaccggaag cgtccgccgt gatgaacggc aggctgatgt
    11761 catagctctg ggtggaggaa agggcgatct tggccttttc cgcttcttcc ttgatgcgct
    11821 gaatggcatc aggctggttg gaaagatcca tgccggagtc tttcttgaac tcgcctataa
    11881 tccagttgat gattgcgttg tcccagtcat cgccgcctaa gtgggtgtca ccgtcggaag
    11941 ccttcacctc aaacactccg tcgccgattt ccagcacgga aatatcaaag gtgccgccgc
    12001 cgaggtcata tacggcaatg ttttcattgg actttttgtc caagccgtag gccagggccg
    12061 ccgccgtcgg ttcattgata atgcggcgca ctttaaggcc tgcgatttca ccggcggcct
    12121 tggtcgcgtt gcgctgggcg tcattgaaat aggcggggac ggtaatcacg gcctccgtaa
    12181 tcgtttcgcc aagtttggat tccgcatcgg ctttcagctt ggccagcacc atggaagcga
    12241 tttcctgggg ggaaaaggtc ttcttttcac cgcccacatc cacgcggatg taggcatctc
    12301 cgttgggagc ctccacaatt tcgtacggca cctttttatc ttcttccgtc agttcactgt
    12361 acttacggcc gatgaggcgc ttggaggaaa acacggtgtt tttcgggttg gttaccgcct
    12421 gacgtttggc ggcctgcccc acgaggcgtt caccgctttt ggtgaaagca acaatggagg
    12481 gagtggtgcg cgcaccttcg ctgttttcaa gtacggtacc ctgaccgcct tccatcacag
    12541 ccatgcacga gttggtcgtg ccaaggtcga ttcctaatat tttagccata tcgttatttt
    12601 agattgtttt gaatggattt cgttgagatt tgatttcctc atgacctgaa gcatcatgca
    12661 ggcaaactca tattcttgcc tcttttgttg caaatggcat gccaatctgg caaggcatac
    12721 tgaaaaataa acgcaaatgt catattataa tcatattacg ctgtttttcc tttcattcct
    12781 ctattttccc gacaaaggaa aacgggacat aatgtcacac cagaaaagag acaaaatgtc
    12841 acacaaagtc attttgtccc acttccgggg gaaaccggat aaaggccgtt ccgtcatgaa
    12901 tcccccgtgc cgggagaagt ttccgcgtcc tccatggttc cgcatttgcc atatgccagg
    12961 cgcttcataa aatccttgta aagcaccgtt tccatacggc cgtcgccgta ttccagcact
    13021 gcgctcatgg tttccaccca gtccccgcaa ttcagatacc tcacctttcc caccatggta
    13081 tccgccggat gatgcacatg cccggcgatg atgccgtcac actgcctctt taccgccagg
    13141 cgttgaagct gttcctcata ttttccgata aaattgacgg cggatttcac gcggcccttg
    13201 atggcgcggg aaacggaata atattccctg ccccgccagg cgcggtattt gttgtaaaac
    13261 cggttgacca tcagcaaaac gtcataaccc agagagccga gcatcgccag ccaccggtga
    13321 ttggtggaaa tggcgtcaaa accgtcccca tgcacgcaca ggtagcgtct tccatccgca
    13381 gcctggtgca cgtactccct ggcaattttc aaaccgccca gatgaaaagg caggaatttt
    13441 tccagaatat catcgtggtt tccgcgcagg tacaggactt ccacatcctc ctgttccatc
    13501 tttttcaaca aggtgcggac aaagcgcgta tgcctccgcc gccagcggct gccgcgggac
    13561 aaagcccagg catccacaat atcccccacc agcacaattt ttccagcccg gacatgtttc
    13621 agaaacttcc ggcattcgtc cgccttgcaa tctttcgttc ccagatgaag gtccgacaaa
    13681 aaaaccgtgc ggcattccag cacggcatcc cgcggggggc gggcgcagcc ggtcccgttt
    13741 tcctcccggg ccacggaagc cagcagttct tcaaacctgt catggatgga atcccaagtt
    13801 ctggaattca ccgtcctgcg ggcctgtttc ccaagcctcc ggatcatctc cccgtcttcc
    13861 agcagacggc gcatggcgga ataaaacccc tcttcatccc ctttctccgc ctgaaggccg
    13921 ttgatgccgt ccagcaccac atccgcggaa gcggcatacc ggtagctgac cgtagccaaa
    13981 ccgctggcca tgccttccag caaaacgttc ccgaaagttt ccgtctcact ggcgaacagc
    14041 aaaacgtcca tggcggcata atggcgcgcc aggtcttccc cacggcgcat tccgcaaaaa
    14101 acggcatcag gaaactcact gcgcaggctg ttcatcatgg ggccgtcccc taccaccacc
    14161 attttcatgc cgcaatccgg aaactccctc tgcaagcgcg tgtacagccc cagggccgtc
    14221 accaggttct tttcccgcgc cagacgcccc acgacgccaa ataccacttc atcccgccag
    14281 acgccccatg actggcgcag ggaagcatcc ctcctggcgg gattaaacag gacggcatcc
    14341 actccgcgcc ccatgacgga aaggcgttca aaccccatcc cctccagcgt acggcgcatt
    14401 tcctccgtgg gaaccactgt catccctgcc ctgttgtgga gtttgcgcag gtaattcacg
    14461 gcggccgttt ccagtctgga gaaatggtaa tccttcatat actggtggaa attggtatgg
    14521 aatcccatca ccacgggaac ctccagcgta cgggcggcct tcacggcaga cgctcccatg
    14581 gggctttccg tagccacgta taccacatcc ggcctttttt tcatccatct ggcgcggaac
    14641 ctgtctgcag aaggcagccc tatcttcacc tcatggtaca tggggagggg aagggacggc
    14701 atagccgtct ccccagggtc gaaagagccg ttccgttcag aagccctcat cacatgcacc
    14761 agatgcccgc gcatccggag gccgccgacc agacgcccca gcgtcagggc cactccattt
    14821 acatcaggtt catacgtatc cgttaccaca tcaattctca tgtaccggta ccatgagcgg
    14881 aatggcttcc tcaaacaaga tgcggccgga aacgaaaccg tcacaaacat ccggttatgc
    14941 tcgcagcccc ggcagaatca acagccgctc cggagcaatt ccgccacggc cttcccgttt
    15001 ttcccgtacg ggtacgtatc atgagccctc tttttttatc aggacatctt ccaaaactgc
    15061 gtattactgc aaaaaagaaa cagcccattg aatatcaaac gctctcaatc catgcgagtt
    15121 catctgcgca tttcaaatcc gcagtcaatc aaattttttg agaaaaaagg actttcatcg
    15181 tatagagaaa gaaaaaaatc acaacgcatc ctgaaagaaa caatccccaa tattgatttt
    15241 atccattgat aataaaatca ataaggaagc accaattcct tttttcgctg acatgcggat
    15301 ttgaaatccg ccacaatgca tgcatatggg atacacaaaa cggaaatgaa tgaatattca
    15361 gaaagacttt taataaaaat aataacgatt cccatgtaaa aaaaacggaa gaatgaggcg
    15421 ggacaacaga cgcgcctttc cgcctttttc acttcagaaa aacaggatgc atatttttaa
    15481 tatgctgata acaaaaaggt taaccaacat ttttcaacct attttaaaca ctgcggatta
    15541 ggaatccgac gtcaatatca gtttttcata ccaaatgcat ttctccaaaa tcatccatca
    15601 tttccccgga tacaaccctc aacagaataa atttgttaga acaaaatata aattattgat
    15661 tgtaatata
//
LOCUS       DAWEMX010000051        15352 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1601_length_15352_cov_10.490096, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000051 DAWEMX010000000
VERSION     DAWEMX010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_15352_cov_10.490096"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3997..4006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14894..14903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3133 a         4992 c         4249 g         2958 t
ORIGIN      
        1 gggctgcgga accggtacgc tgacgcacgc tctgctggag aaatcggcgt ccgtcaccgg
       61 cctggactcc tctccggaaa tgatagcaaa agcccgccag ctctatcccg gcatggactt
      121 ccgcgtgctg gacgcccgcc tcatgccctg gcgcaacaga tttgacatcg ttttctccaa
      181 tgcggccctc cattggatac cggaccatgg gactctctta aatgccgttt cccgcgtcct
      241 gaaaccgcag gggaaactgg tctgcgaatt cggagcccac ctcaatattc tccgcatcag
      301 ggaagctttt cggacagccc tggaacgcag gaacctcccg tacacaacgc gtttttactt
      361 tcccgccgcg gaagaatacg gcagcgttct ggaacaagcc gggctccgcc cggaaacaat
      421 gaaggaattt gaccgcccta cgccgctgaa aggcggtcct gacggcctgc gcaactgggc
      481 gtgtcaattc ttccgggaag acttgaacaa cctccgcgaa gaacagcgca tccagatttt
      541 caaggaaatg gaggattccc tgaaagatga gttatgggac ggttcccaat gggtggcgga
      601 ctaccggcgg atccgggtga cggcagccag ataacccgga gcagtccccg caagaaagag
      661 aaaacgcgcg ggcattccgg atacgctgat aatgcttgag cctcctccgg gcattttgta
      721 caaaagccgc caatgagtac gttcactcct tttgcggaaa agatggagtc cgccggcgtt
      781 tcagccgcgg ccgtcaacgc tttctcccgt tgttatgaag cccttgtttc caaccactcc
      841 ggcatgattc cggaaacgga catcctgccc gccgaccagg tggaagactg gcaggacatc
      901 acggcctcca cccccgcggc ggacaaggac ctgatttccc gttgcgtatg catcaagctc
      961 aacggcggcc tcggcacgag catgggcctc cagaaagcga aaagcctgct gaaagtaaaa
     1021 ggagaggata cgttcctgga tttgatcgtc aggcaggtga agcatctgcg ctccatctcc
     1081 ggcacgcctg tgcgccttct gctgatgaac tctttctcca ccagcgcgga cacgctggcc
     1141 tacctggaaa aatacgcggc ggacggcttt gcggaccgcg ccgaagtgga gctgctgcaa
     1201 aaccgcgttc ccaaaatcct ggcggacggc ctctccccgg ccagctgccc ggaacagccg
     1261 gagctggaat ggtgcccgcc aggccatggg gacctgtatc cggccctgct ggggtccggc
     1321 tggctggacc gcctgctgga ggatggcgtt aaatacgctt ttgtctccaa ttccgacaat
     1381 ctgggcgccc agcttgacat gaacttcctc cgctggtttg cggaaagcgg cgcccccttc
     1441 gtcatggaag tcacccgccg cacggtggcg gacaggaaag gcggccacct ggctgtcagg
     1501 aaatcggacg gccagttgat tctgcgggaa gtggcccagt gcccggatga ggacattcct
     1561 gaattccaga acatttccag gcaccgctat ttcaacacca atacgctctg gatacgcctg
     1621 gacgccctca aggaaattct ggacgccaac ggcggcgtac tcccgctgcc catgatccgt
     1681 aataaaaaga cgctcaaccc ccgcgatccg gaatccgccc cggtgtacca gctggaaacg
     1741 gccatgggcg cgggcatcga atgcttcccc ggcgccaggg ccgtcaacgt cccgcgttcc
     1801 cgtttcttcc ccgtcaaaac cacttccgac ctgttcctgc tccgttcgga cgccatcgcc
     1861 gtggatgaac acggcaacgt ggccctggcg ccggaacggc agggcaacac cccggtggtg
     1921 gacctggatt ccaaactgta caagctggtg gattccctgg acgggctggg cctgccctcc
     1981 ctgataggta tggacaagct gaccctgcgc ggccgcttcc acttccagga cggagccgtt
     2041 ctccggggca ccttgctgat ggaaaacgac tccgacgaaa cgaaggaaat cctcccgggc
     2101 gtctatgccg gagcctgaac gccatcttcc cgcttccgcg ccccgtccgg ttcaggccgg
     2161 tctgggcgtg gaggacatcc ggcacattcc cgtccggaaa cgggagttgc gcttcacccg
     2221 gaaccggtca ggcgccatcc tgacggcggc ggggttcctt ctggcagcca ctgccgcttt
     2281 cctccagctc acgggatatg atacaattac cccgtatttg cccgcccccc tgtgggccat
     2341 gcaggccgcg gcgcttgttc ccgccgccct ctgcctggct gcggggagcc gctgcctgaa
     2401 acacgcggcc gtcatcgtca ccccggtggg agtggaaatc ctgccgttcc tgagaacgca
     2461 gcgcaccatg caatggttct tctggcagca gattcgctcc gcggaccggg aaggcggacg
     2521 gctgaacctc cgcctgactg acggatcaac cgttgccgtc agccttcgcc ccatgacggc
     2581 cgccagccgg gacatgctgg cccatgctgt ccggcagcgc gttaacacct tgcaatcctc
     2641 cggctatggt caggcctgaa aaactggcag ctctccggga acggatggag gccctgggca
     2701 tccgtgaaca ggatctggag gaaagctttg tccgcggaag cgggcgaggc ggccagaaag
     2761 taaataaaac caacaactgc gtttacctca ggcacacgcc cacgggcatc gccgtcaaat
     2821 gccatgcgga cagatccagg gaactcaacc gctttctggc gcgcagggaa ctgtgcgatg
     2881 ctgtggaaca aatgcaaacg ggccgatgcg ccgcaagaac ccgcgtcatc cagcgcatga
     2941 gaaagcagaa agacagaagg aggcggaggc gttccgcccc tgccccctct ccaccggaca
     3001 gcgaataaca gaacggaaat ccggaatctc cgctaggaac gcggaaaaac ctcacggtac
     3061 ttctccttca acatggcgaa tacattgtgg ggaacatagc ggctcacctg gccctcccag
     3121 ccttccggcc caatcaaccc tttcaccatg ctggaggaca actccgccgt atcccgggga
     3181 ggcatcagga acaccgtaca gaccttgggg gccatatccg cattgatatg gcgcatcacg
     3241 cgttcatact cgtaatcctg cgtggaacgg atgccgcgca gcatgaacgt ggccccgtgt
     3301 tcattggcaa aatccaccag aaagcggttg tggaactcgg caatgcgcac atccggcatg
     3361 tcggaaagcg ccacccgcag catatccatg cgctcctcat gggaaaacgt ataacgcttg
     3421 tccggattat cgcccatggc cacaatcagt tcatcaaaca tcctcgcccc ctgcctgatc
     3481 atccataaat gaccgttggt cagagggtcg aaactaccgg catacacggc gatgcgcttc
     3541 attcgtgcca tcctatacgc ccaccggagg aaaatacaac ccttttccat gcggcggaac
     3601 tttccttcac gtccaaaccc gctcttttcc ggggttccct gcctgacaag ccagcaggaa
     3661 cgtatcaacc cacggcacct gccgcctgcg gcacgaatac gtggcggcct tcctcccggc
     3721 ccaccggaat gaagccgtac tgacggaaaa aacggtccac gcggcgcggg gaagattttc
     3781 atcccccggc ttgtcaagag gccgtatttt ttgcatcatg tttctccgtt atgcagaaac
     3841 gacgcgttgt catcctgggg tccacaggtt ccatcggacc cagtgccctg aaagtcgccc
     3901 gggacattcc ggacagaatg gaaatcgtgg ggctggccgc aggaaccagc gtggaggcgc
     3961 tggcccgcca ggcacgcgaa ttcaacgtcc gcaacgnnnn nnnnnnatgc atttatgacc
     4021 cgtccggagc tgccggtctg gcggcctccc tccccggcgc gcaggtggaa acgggagaag
     4081 aggggctgtg ccgcctggcg cagctcccgg aggcggacat ggtattaatc tccatcgtgg
     4141 gaaccgccgg gctcaaaccc gctctggccg ccattgaagc aggcaaggac ctcgccatcg
     4201 ccagcaagga aatcctggtc atggccgggc aaatcgtcat ggaaaaagcc cgccgggccg
     4261 gtgtgcaggt gcttccggtg gacagcgagc acaacgccat tttccaatgc ctgaacggca
     4321 gccacggcgg cccggaagcc gtctcccgcc tgattctgac ggcatccggc ggccccttcc
     4381 gcacctggaa cagggaagat ctggaacacg tcacgctgga acaggccctc aagcacccca
     4441 cctggagcat gggccgcaaa atcaccatag actccgccac cctgttcaat aaagggctgg
     4501 aaatgattga ggcccgctgg ctctttgacg tccccatgga aaaagtggac gtcatcgtcc
     4561 acccgcagag catcgtgcac tccatggtgg aataccgaga cggcacggtg ctggcccaga
     4621 tgagcagctc ggacatgtgc tttcccatcc agtacgccgt cacgtggccg gaccgcgttc
     4681 ccaactccct gaaacagctc aatttctcgg aaatcggccg gctggtgttc gaggcccccc
     4741 gcacggacgt cttccccgcc ctggacctgg cgcgcagggc cggagccggc aacagcaccc
     4801 tggcagccgt ttacaacgcc gccaatgaag cggcggtaaa tgcctttatc cacggccaaa
     4861 tctctttccc cggcatctgg aaactggtgg aggccgtcat ggacgaccac accccggcgg
     4921 atccctgcgg ggaactggcc cccattctgg aagcggacca atgggccagg aggcgcgcgg
     4981 cggagctgct cccatccttt tcccgcccct gctgacgcca tgaccgcccg gactcccgcc
     5041 ggaacgccgc ccccggccgc gcgcatcacc attctggata tcctccgggc cctggccctg
     5101 ctgggcatcg tcatcgtcca cacgcacgac cacttcaacc tctatcttcc catgcccccg
     5161 gcggaaggat ggcaggctgc ggccaacagt gcggcggact gggcctatga acatctcttt
     5221 gtcagcaaat ccttcctgct cttctccttc ctgtttggcc tcagcttctt catccagctg
     5281 gacaggcggg agcagcaagg aatcgacttc cggaaacgct tcatgtggcg cctgacgctc
     5341 ctgttcctgc tggggctggc gcataccctg ttctatgacg gggacatcct caccctcttc
     5401 ggcattctga gctttgccct ggtcatgctc tacaaatgcg ggacacccct gctcatcacc
     5461 ctctgcgccc tgtgcctgct ccagccggtc atgctcatgg acctgctgaa ccatgccggg
     5521 ctggcggact ggtggcccca ctcctccggc tggttccata cggcctcccc ggctgcgagc
     5581 cccactcggg aattcctgta cgcccacggc agctggggcc aggcggccct gtggaacctg
     5641 acgcagggac aggcgggcaa atggcatttc ttcctgctca gcggccgcct ctggcaaacg
     5701 ctgggcctct tcatcctggg catgctggcg ggcagatggc gcacctttga agacgcgccc
     5761 gacaaacgcc gcctgttcct gcggatgctg ggaatctccg gaaccctgtt cctggctctg
     5821 ctggcggcgc gcctgttact cccctcccgg ctggactccg cgctggaagt ggacgcagga
     5881 caccttctgc accaatggga aaacctggcc tataccgcgg ctttcgtctc cgccgccgtc
     5941 ctgctgttca cccatcccgg cctccccctg cctgccagac tgctcagcag cgccggcaaa
     6001 tgcacgctga cctgctacat cacccagacg ctggtcttca ccttcctctt cttcgggtgg
     6061 gggctgggcc tggcccagga catggggccg tggccatgcc tgtgcgcagc cgttgccgtc
     6121 ttcctcctcc aggcctgggc ctgccgcctc tggctaagcc atttcctgta cggccccttg
     6181 gaatggctct ggcgcaccgc caccatgtgc cgcatgcagc ccttccgcaa gcggatttcc
     6241 cagaaaaatt gagatattac tctccttctt cctgttcaat cccggtttct ccgaacgtag
     6301 gaggaatcaa acgcacagcc tcctgcgtcc ggtccggaaa gaaaccattc ctttcgtaaa
     6361 aaggaacctt ttccctgcag gaagtgagaa ctatgcgcaa atacccggca tagcgtcttt
     6421 tgcacatacc cagcaaccac gaaccgacac cgcatccctg gtacctaggg atgcaccagc
     6481 agataatgga cataagccgt catggttccg tcatccatgg cggctaccag ccccgccagt
     6541 ctgctcccat cccaagcgga gaacactgcc ccgtaatggc gcaatgcctt cgccaagcgt
     6601 tccggatggt ttccggattc ccattgaact gaaagaaaca attcctccag cgcctccggg
     6661 gaaaattccc tagtatcatt atagaaaaga gccatggaaa atcatctgcc cgtaaaaaag
     6721 gctttccacc cttataaggg tggaaagcct gaaaaagaaa aaaatgccgt cttactcgtc
     6781 atcctccgcg tcaaaggcgg aatcttcgga atcaaaatcc ttatccaggt gggcggtgaa
     6841 tttcttcacg cgctgctggg cggggagcgg ggagagcacc atgtgcatgg tgttgccggc
     6901 caaacgcggg gcctgatcca cctgcgccat ggtcttcata tccgcgatga ctttgttgaa
     6961 gacgtcgtag ccaagctcct ggtgggcgtt ctcacgaccg cggaaacgga gctggaagcg
     7021 gaccttgtgg ccatggtcca agaagctttc cgtacgggcc attttcacat tatagtcatt
     7081 ggtatccgta ccgatgcgga gcttgatttc cttaagcttg acggttcggc tcttgttgtt
     7141 tttctggagc ttggcctgct ggtatttgta cttgccgtaa tcaaccacgc ggcatacggg
     7201 cggatcggca ttgccggcca cttccaccag gtccagcccc agagccttgg ctttttccag
     7261 cgcgtcgcgc gtgttcatga cgcccagctg gtcgccgttg gcagtcacta cccgcacgcg
     7321 gggcgcgcga atccgttcat tgatccgagt ctgatcgccg cggtcacggc ggcgatttcc
     7381 gtcattattc ttcgttggct tggttggcac gtccggatac tactaaacta aaaggaataa
     7441 cttggcaact caaatcacgg gagaaatatg acgctccgcg atttcccggg ctacgaggtc
     7501 cgcgaattgc tcaaagggca tcgcgcccag atcccccttg tcccggtggc ggacggagac
     7561 ggtgccgtct tccgcctccc gctggccgag caccagcatg tagggaacgc ggtccagacg
     7621 ggcattgcgg attttggcgc ctattttgtc cggcgtttcg tccaccgtga cgcgcacgcc
     7681 tctggcggcc agggcggttc gatgcgcggc ggccacgtcc gtcaccttgt cggagatggg
     7741 aagcacgcgc acctgttccg gggaaagcca ggtggggaat ttgccttcaa agtgttcaat
     7801 cagaagcccc gtgaagcgtt ccatggagcc gaagggggcg cggtgcacca tcacgggccg
     7861 gtgcggcttg ttgtccgctc cggtgtaatg gaggtcaaag cgttccggca ggttgtagtc
     7921 cacctgcacg gttcccagct gccattcacg gccgatggca tcgcgcacga tgaagtcgat
     7981 cttggggccg tagaaggcgg cttcccctgc ttcctcgctg tagtccgccc ccagccattc
     8041 cgcagcttcc cgcagagcct gttccgcttt gtcccagttt tccggagaac ccacgtattt
     8101 gtcgctttcc gggtcgcgca tggaaaggcg cacgcggtag tccgtcatgc ccagcgtacc
     8161 gaaaatggtt ttcacgatgc ccaggcactg gcggatttcc ccggcaagct ggtccggcgt
     8221 gcagaagata tgggcgtcat cctgcgtgaa accgcgcacg cgcgtcatgc cgcccagctc
     8281 tccgctctgt tcccaacggt acaccgtgcc gaattccgcc agccgcacgg gaaggtcccg
     8341 gtaggagtgg gcgtcattcg cgtaaatctt gatgtggtgg gggcagttca tcggcttgag
     8401 catatagccc tcaatggtgc cggtggccag gccgttgaac agttccgcgc aggaagcccc
     8461 ctcctggctg agtttttcca tggcgtcccg ttccgggatg ggcgcgtact ggctttcctg
     8521 gtaatacggg aagtgtccgg acgtgcggta caggtccagc ttgccgatgt tgggcgtgta
     8581 cacctgggag tagcccagct tgtccagctc ttccgtgatg aagtcctgca gggaacggcg
     8641 gatgagagcc cccttgggct tccagaggat gagcccctgc cccacggatt cgtcaatgca
     8701 gaacaggccc agttcgcggc ccagtttgcg gtggtcgcgg cgccgcgcct cttccagccg
     8761 ttccaggtgt tcgtcaagct gggtgcggtt cgggaaacag gtgccgtaaa tgcgctggag
     8821 catggggcgg tttttgtccc ccttgtagaa ggcgctcgcc acgctcatga ttttgaatgc
     8881 cttgcagttg cccgtgcggc ccacgtgggg accggcgcac aagtccgtaa aatccccgtt
     8941 gcggtagagg gaaatctcct cgtcttccgg gatgtcgttc agcaggtcaa tcttgaagcg
     9001 ggaaggctcg ctgcgggggc ccagggcgcc cagctccccg gattccgcca tcttcatggc
     9061 atccgcgcgg gaaatgactt ccttctggaa aacctggttt tccttcacca cttttttcat
     9121 ctcctcctca atgcgttcaa aatcctctgt gctgatgcgg tggtccagct ccacgtcgta
     9181 ataaaaaccg ttatccacgg caggcccgcc ggccagctgg gcatccggcc agaggcggca
     9241 aatggccgta gccagaacgt gcgcgcagga gtggcgcagc cgttccagat cagacatctg
     9301 ttggcgttct tcaagagtct ttctttcctt gtgttcggac atacgcgcat cagtaaacct
     9361 ctcttaaacg ctgttggcaa atgcaaaaag accttcttcc cgaaaattca aggcatgaac
     9421 gccgcgggaa agccctttcc tccggctttc ggattcttct tccggaacgc atcctttccc
     9481 ccctttttgt tcgcttttcc ccggagtcca gggttgtccg ccctgcggca acggccgcgt
     9541 caacattccc aacgaatgat ttacacccgc agccgtttat gccatagtgg cggccagcgg
     9601 tcatgaacat cctccccctc tcccttttct gcctctgcct ctgctgcggg ttttcccggg
     9661 gcggccagcc gccggaaggc gctcctgtcc gtttccggat caatatggat caggctgcgg
     9721 gaaacaaggc tccgtccacg ggacccgttc cggaccagag cacgccgttg aacgcggcca
     9781 aactgctgct ccgcgccctt caggaaaaaa cggactggaa acacgagccg tacaagtcat
     9841 tgcccgccac ttcccccttt gtaagccaac tcaggctgaa aagcatcctt tcccgcatgg
     9901 aggcggtcca ggggtatgtc accggagcgg ccccggaacg tccgcccttt gtggatgaag
     9961 actccatcat tgccgaaggc gacctggcga tggcctacat cctgctgccg gacaaggcca
    10021 acccgtacgt ttatgcggcc accgccgtgg ccctggtgaa gaaagaaggc caatggaagg
    10081 cctccctgac tcccggaagc ttcgacaaca catttcttca ctttgaggac ggcatccgtg
    10141 agaaagcgaa aaaaatatcc tcggaggcca agaagaaaat tttcggcctc gcccaccagt
    10201 actccgtagc cgccgttaaa gccgccctgg aacatatcaa gcgtttccgc caggaaaacg
    10261 tcaggggaac aacggacgac gaactcaaga acctgctcat ccgggccgtg aagggggatg
    10321 acccggccct catcgccgcc ctgctcgttt ccccgtatta tacggaatcc gccattaccc
    10381 agagcgcacg cctcattccc gtggtcaagg ccatgcgcct ccaggacaga agggaggaaa
    10441 accgctatat ccagcctacc cacctcagct tcatgacctt tccgaatact atcctggttc
    10501 ccctgagccc ccatccggac gaccagatgg ccccgaacag ccgtgaagaa gaccagcagg
    10561 aggaagccaa taaaaacaac gcgaagaaga acggcaagga catccacagc atcggagcca
    10621 tgaccatcct gccgccgcgc atgagcatgc cggacataga caaacccgct ctcatttacc
    10681 gctatgcgct ggaaaaaacc aaggatccca cagacggcct tcccgtcttc aagatgaacc
    10741 tgacggacgc tttcgcttcc tggaatctga aaccggacga atccaccacc gcccgcatca
    10801 tggcggattt ccaccggacg tatcctccgc tgtcctttcc cacgccggaa gaggccgtaa
    10861 ccgccgccgg ccgcgccctg gcgaaccggg acagcctggc catgatgcgc atgctggccc
    10921 cgtccaactt cgcctccgtg aaggaatttg aagacgcgct ctcccaattg aaaacagtca
    10981 tgggaaggat tcccaccccg cacaccaggg taaggggaaa agacgttccc gaagtcaaag
    11041 cggcgtatat tcccgccgcc agagacggct gcgccggaat caaggccacc atcggcgtgc
    11101 gtacagcagt tggaaatgaa ttctccgtca acaacctgca cttcatccaa acagacggcg
    11161 gctggatgct cgccggggcc gatgcggaac agccgctgga actggaggcc cccaaggctc
    11221 cctgatcata tccgtcccgg aacgccatgc cttcctaaaa accttttcat taccacacca
    11281 tgatgaatct accgatacgg cctcgccgca acagaaaatc cgccaacatc cggggcctta
    11341 tccgggaaac ctccctttcc ccggaacacc tcatctatcc cgtcttcgtc catgaaggga
    11401 ccgggaacca gcccatcccc tccctgccgg gctgcacgcg ctggagcgtc aagggactgg
    11461 tggaggaagc caaacgcctg atggatctgg gcatccggac gctggatctt tttccagcca
    11521 ttccggatga gaaaaaaacg ccggacgcct gcgaagcctg caaccctgac ggcctcatcc
    11581 cgcgcaccat ttacgcgctc aaaagcgaag tgccaggcat caccgtcatg acggacgtag
    11641 ccctggatcc ctacaattcc gacggccatg acggcctggt ggaattccgc tccgacggaa
    11701 cgatggaaat ccttaacgac gattccgtgg aggttctctg ccgccaggca ttgtgccacg
    11761 cggatgccgg ggcggatatc gtctccccca gcgacatgat ggacggccgc gtggccgcca
    11821 tccgcgccac cctggattcc gaagccctgg acgatgtctc catcatggcc tataccgcca
    11881 agtacgccag cgctctgtac gggccgttcc gcggagctct ggagagcgcg cccaaggaag
    11941 gggataaaaa aacctaccag atggatcccg gcaacatccg ggaagccctt agggaagcgc
    12001 agctggatga agctgagggt gcggacatcc tgatggtgaa acccgccaca ttgtatctgg
    12061 acgttatggc cgccatgcgg aagcaggtga cccttcccat agccgcctac catgtcagcg
    12121 gggagtacct gatgatcaag tccgccgcag cttccggctg gctggatgaa cgggaaacag
    12181 ttctggagac tctaatatcc atccggcgcg ccggggccga tatgatcctt acgtattacg
    12241 ccccccaggc cgccgaatgg cttcaacaac gctgacgccg cacggccacc gtaccggagg
    12301 tggacgtacg gttcagaaca ccgccctccg catgtccacc tccacgaaaa tcattgtcgg
    12361 catcgtgatc gtcgtcgtta tggcgctcac ttccttctgg ggtgtttacg ctccttcttt
    12421 ttcggaagcc tatatactgc ccgtccagat gacggcggct gacggaacac cgcttggcga
    12481 cttccagtgc cgtcctgtac ccggggaaaa cggaaagacg ctcctggtct tccgggatgc
    12541 ggatttcctg cgccagacta cgggcggcat ggtgcattcc aaaatcatcc tttcctacaa
    12601 aggcacgccg ttctttgaag gaattgtccc cggtcccatg gaagccattg aaattccctc
    12661 cggcagcctt cctccccctt tccgggaact ccggcataac tgaggatgcc cccgggaaga
    12721 cggcctgtta tggcattgtt actgaaaggg cgcaggtttc tctatacaac aggggaaata
    12781 ttgtggtttt atagaatcaa acgatgagtg attcagccta taccattctg attgcggagg
    12841 acgacgacag catcaggcat gccctgacgg acgttctgac cgcatccggc tacgaagtgc
    12901 tggctctgga agaaggcctg gccgccatcc atgccgtgcg ggaacgcaat ttcgacctcg
    12961 ccctgctgga cgtggccatg ccgggcgcgg acggattcca ggtgctgcag gtcatgtctg
    13021 aggaacgccc cggaacgcct gtcatcatgc tcacggcccg tggagaggag gaagaccgcg
    13081 tgcaggggct caagctgggg gcggacgact atattgtcaa gccgttcagc atccgggaac
    13141 tgctcgcccg catcgaggcc gttctgcgca ggtctccgga acgaccgcgc cagctccggg
    13201 aaatcaggat tcccggcgcc accctggaca gcgccaaccg cctgatcacc tttaaagacg
    13261 ggagggaaac ctccctgacc gcgcgggaat tcgaattcct cacctatatg gccacccacc
    13321 ccaaccgcgt tatcacgagg gacgaactgc tccggcgcgt atgggacgtg gacccgcgcc
    13381 tgacggatac gcgctccgtg gaaatgaccg tcatgcgtct ccgggacaag ctcggggccg
    13441 cctccgcctc ccccctggag acgcttagaa gccagggcta ccgctggaat tcatccttcg
    13501 tttcctgatc cgtgaaccgg cgcatacgca tcattctgct ggcaatcagc ctgctgctgg
    13561 tcatcggcac gctcggatgg ctcacccatg ttctgctggt gcaggcggaa gaaacaggcc
    13621 attccgaaca gcgtatcagg gtggaagccc tggcccatga agccaagctg gaaatggacc
    13681 gccttctgac ctccttcatc acattcgaac aggcccgcgg ctattttgaa taccagcccg
    13741 tttacgccta caaacgcccc tatgacctga ggatgggcgc cccggccccc ggagaagcaa
    13801 cccgtggttc caatctcgcc gcctacctgc cggactacgt tacagccttc attcagattt
    13861 cccctgccgg gcgcgtaacc ggccccgctc ttcccccgga gcttgccggg cggctgaatg
    13921 aacagaagga cctttaccag cggctgaacg gcaaggtctc ctcccttacc tccgttccgt
    13981 ccagcggaaa cctctggacg gatatccgga atgaaattct gcatgaatct ccggaaccgg
    14041 agccaacggt atccggcgct ccggcacaag tggaaactgt cacttccttc gttcccgtgg
    14101 aagacggagg gaacctttac atcctgcgcc aggtgcacac cagccgcggc atttatttgc
    14161 agggggccct gatgaacatg gacaacctga acagacgcct gcccggccag gtaaagatgc
    14221 tgcttcccca cagccgcctg gcaaccttcg acccgtcctc gccgcccccg gaggaaaccc
    14281 ggcaggcggg cgcactcccc tttgccaacg cccccctgat tttccttccg ggagacacca
    14341 gagccgttcc tcttcaggac cacaaaaaaa tgctgacctg gacgctcacc ctcatctgga
    14401 gcatggtgct cctgggtgcg gctggggtca tctggctcat gctgggaacc ttccgcctgg
    14461 aacagcgccg cggggacttc gtctccgccg tcacccatga actacggacg cccctcacgt
    14521 ccttttccct gtacacggaa atgctggaag acggcatggt gccggaacag aaaaagccgg
    14581 aatactatgc caacatgcag cgggaatgcc gccgtctgga acacctgatt gaaaacgtac
    14641 tctcctactc ccggcttcag cggaacgcta ttcgccgcgc gcgggacacg ctcacctgtc
    14701 aggagctttt tgaacccatt gcggaaaaaa tagaacgccg cctccgggag gcggacatca
    14761 gcttctcctt tgccctggcc cagcccatcc gcatccttcc tattcatacg gatgccgttt
    14821 ccgtagagca gattatggac aacctgactt ccaacgccat taaatacgcc aagagggaaa
    14881 acgccaaagt ccannnnnnn nnnttccgcg acaacggccc aggaatttcc ccgaaaaacc
    14941 gcaagcttgt cttcaagccc ttccggcgca caaaagacgc taccaacagc cggaaacccg
    15001 gcgtaggcct ggggctggca ctggcaaggg acacggcacg ttccctgggg ggagacctga
    15061 aactggaatt cggcaccctg gggggagcca gctttctgct gacgattccc aaatcctgaa
    15121 cctgccagcg ggacagccgg gaaccaaccg gaattcgcct cccggtccgt acggaaaaac
    15181 cttcctggtt cctcccctgc ctttcccgga catccctgta atatcccgtc cgggatgcta
    15241 tgcgcttgcc ttcccgtgac ggatatgtga cattgaaccg caacgcacgg acaggggaaa
    15301 actttccgct tgcttccctt ccggcgcggc ggaacgaaca aacgcacgga ca
//
LOCUS       DAWEMX010000052        15331 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1605_length_15331_cov_23.754779, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000052 DAWEMX010000000
VERSION     DAWEMX010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15331
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1605_length_15331_cov_23.754779"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    725..734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3151 a         4792 c         3761 g         3617 t
ORIGIN      
        1 gtaggaacca acgccggaca aatcaaaacc ggctctttga gccgttccga ccgtctggcc
       61 aaatataacc gccttctccg gattgaagaa gaactgggag aatccgcccg ctacggcaac
      121 ggattcggcc tgttctgacc tttttgctgg aactggtatc aagcaacgcc aaaccccggc
      181 cgccgcgagg cggtgcgggg atttttcatc tccggaggca actgcatcct tttctttgaa
      241 acaaaatatg ccatgctggc ccttcggagt cctcccctgc cgataagttt tccgctctgc
      301 gtcatgcctg cccgcttctt ccttcccgca cgccttccgc tatgcgcggg gttgttgtac
      361 ctggcgctac ccctcctcct ttttctgctg ggatgggtgc atccggcttt ttccgtaccg
      421 ctttgcgccg cactggcatg cgggctgtac gcctgcgctc ggcatcttcc ggcacaaagg
      481 tttccgttaa gcgggcgcgg attggccgtg ctgggcctcc tctccttctt ctgcctgatg
      541 ctggtgctgt tgtgcggatt cacgggccac tgccagcaac acgcggattt tattatccgc
      601 aatgccgttt acgaacatct tgcagcaaat tcctggcccc tcgtcacgga ggacggacat
      661 catttcatct actacctggg ccattggctg cctccggcgc tggcggcttc tttctgcccg
      721 gaatnnnnnn nnnnttggcc ctgtggacct ttcttgggct ggaactggcg ctgctggcgg
      781 caaccgtccg ctggggaatc cggaagacgg cccgctgggc gctgatcctc ctctgcctcg
      841 gctctccggc agcggtgccg gactgcctgg gaattcccct ctcctccctg tttgcggaat
      901 acaatgccca gatggtcctg tttatcggca tgcccgtcca actcttcaac acattcaatc
      961 acgccgttcc ggcgctattg tgcgccgtat tcgtcctgac gcgctccctg cctccctccg
     1021 ggtattatct cgcgggaacg cttctgctgc cgtcctcccc gctgggcgcc cttctgctcc
     1081 ttccgtatat ggcgtatgaa accctgttcc gccgctcgtc ggcacggaaa cctctttccc
     1141 gtctccgttc ccttctgggc cagccggttt tctggctcgc ggccctgtgc acggccgtca
     1201 tggccgtctt ttattcccat ttggacggag gagggcaatt ttcctgcctg tttgacgcaa
     1261 aatatgcgga aatctaccat tacgggcagc agcggcttct gctccatccc ggttccgcca
     1321 aatacgccag cttcctgctg gccctgaccc tggggatcct gctgccgggg gcgctcctct
     1381 tcccaaaatg ccggaaaaac ccgttgtact acatcacgct gggcatgatg gcaggcagtc
     1441 tctttttccg cacgggcatc atgaacaatg aacttctctt taaagctccg gccgtcctct
     1501 atcccttcct ttccctgctc tttctccggg ccctccgccg gggaggcgtg aaataccgga
     1561 ccctcctggt tctttatctc gttttttccg ccctcccgaa cctgcaatgc atcatggaaa
     1621 aagcgggaac attctccacc cgggcgagca tcatgcggaa acattggcag acggccaacg
     1681 gcacgcctga ctgcccggac actctcatcc gccgccagtt catgaaaaag gacggccatc
     1741 cccttccctc ctggctattc aaaaccggaa caaagccaca atcacggcgg caataatcaa
     1801 tccaggctga tgccgaatat atccgaaagg gaactggaat ccacatccag tgcgtcttct
     1861 cctccgggaa cgggttccgg agctttcgga atcaaaacgg aggaatccat tccgcgcagg
     1921 gtgaacaaca actccggctg cttatccagc atcacgccga aagcgtacag gactgcggcc
     1981 gcatgcttgc acaaatccgc ccaatccgga caggaacagg aaaaacgcca ctcttctggc
     2041 gcgggaaaca ggccgccttc cggagcgcac aggatttcca gcagctcggg agacaaattc
     2101 cctttcagca gatcaatcca ggaaccaatc tgcccggcgc atcgtgcgcg cagccgggca
     2161 agcgcctctt catccggggg ggaggcctgg atgcaaacct catacaaatg ttcacccatc
     2221 accagagcat cgatgcggcc gggaaccgtt ttaaggtcca gaacacagcc atagcgcagg
     2281 taggtccggc cgggggccag ccgcatcccg tacacctcgc acgcagacag gctcttcatc
     2341 cattccttcc cccagaacct tttcgccagg gtacgcccgg aaaccgttat ggggcgcagt
     2401 tccatcccct ctgcagccag ttccgccgcc ttttcctccg cgcgtttccg caaatcttcc
     2461 cgtttcagag gcatttcatc atccatacgc acggcatcgt aataacatat ccgcccccgt
     2521 ccgccacagg gaaaagacag ccatgctccg tttctctctt ttccggctcc actccggttt
     2581 tccaggcatt cggatttttc ccagttccgc ggaatctgcc tccattaccc atttcaggct
     2641 ttcaaaacgg aagctcctgg ggcacaacat gtgggtcatg tggaaaggca gattttctaa
     2701 acccaccgct gatctggttc aacgctacgg cgaatccgtc tcctacgact ggaggctttt
     2761 ccgccaggac attgccggat ccatcgccca tgcgcgggcc cagctcaagg ctggcctgct
     2821 ttcgcaggaa gaattcaacg ccattgaatc cggcctgaaa gacatactga aagacattga
     2881 agcaggcaac ttctcgtgga gccgggaact ggaggacgtg cacatgaaca ttgaaagcga
     2941 actcacccgg cgcatcggcg ctccgggcgc caagctgcat accgcacgct cccgcaatga
     3001 ccaggtagct acggacacgc ggctttactg ccgggcggaa atcgatgaaa ttctgggaaa
     3061 agtgcgccat ctgcagcgcg cgctggtcat gaaggcgaag gaatacgcgg atgccatgat
     3121 gccgggctat actcacctgc agcgcgccca gcccgtcacc atgggccatc acctgctggc
     3181 ttacgtggaa atgctggacc gggatgcgga ccgcctcaag gactgccgca ggcgcctgaa
     3241 cgtctccccg ctcggttccg gagccatcgc cggttccacc atctgcctgg accgccacgg
     3301 aattgccgtg gaactgggtt ttgacgcagt tacagaaaac tccatggatg cgattgccga
     3361 ccgggattac atcatggaaa tcctttttga cctggccgtg gtcggagccc atctctcccg
     3421 catgagcgag gacgtcatcc tgtggtgcac ggcggaattc ggcttcgctt ccttgtccga
     3481 cgcctatacg acaggttcat ccctgatgcc ccagaaaaaa aatccggacg ttgcggaact
     3541 cacacgcgga aaaacggggc ggctgtacgg taacctcacc gccgttatgg tggctgtcaa
     3601 agggcttccc ctgacttaca accgcgacct ccaggaagac aaggaacccc tgttcgattc
     3661 cattgatacg gttaaactgg ccctggacgt caatgcggaa atgatagccg ccatgacggt
     3721 gaacacggcc agggccaggg aagcggcgtc cgaccccatg ctgctggcca cggacctggc
     3781 ggattatctg gtgcgccgcg gggtcccctt ccggaaggcc catgagctgg tcgggaaggc
     3841 agtcgccatg agcatctcca cgggtacgcc gctcaacaag ctgagcgacg aacaattcgc
     3901 ctccatttca aatgcatatg gaacagacgc gcgcgacgta tttgaattga caaaagcctt
     3961 cgcccagcgt accaacccgg gggctcccaa ttccgacaac acgcgccgcc gcatcgccta
     4021 ctgggaaggc atcctttcca agtaacgccg cctgcggagg cggacggaaa cgaagagagc
     4081 ctgccgtccg tctccgcggg gcgcctccgg gtgcaggaag gggacgcttc aaccccgccg
     4141 gcggcggcca caaccgcagc cggccaacca tcccggcagc catgttcaaa cctgctcttc
     4201 tctcctctct caaaacttat accaaacaaa cctttctggc ggaccttttt gcgggactga
     4261 ccgtcggtgt agtagccatt ccgctggcca tggcctttgc catcgcatgc ggactctccc
     4321 caacccaggg cctcatcacc gccattgtgg ccgggttcct catctccctg ttcagcggaa
     4381 gcaaatatca aataggcggc cccaccggag ccttcgtgat catcatcatg ggcgtcctgg
     4441 agcaatacca cgcatccggc ctgctggtct gcacattgat ggcgggcctc ttcctcatca
     4501 tctttgggtt ctgccgcatg ggggcactca tccgctttat tccattccct gtcaccacag
     4561 ggttcacctc cggcatcgcc gtggtaatct tttccacgca aattaaagac atcttcggcc
     4621 tcaccatcac ggaaaaaatt cccggagagt tcattgaaaa atgggcgtgt tacttccatt
     4681 acttccacac catcaactgg gcggcgctgg ggctggccgc cggcaccgta attattaccc
     4741 tgctgagccg ccgcttctgg cccagaatac cggccatgct ggtgggcatg ctgggcatga
     4801 cggccgtttc cgtggcgttt tcgttgcctg tgacaaccat cgggcaagcc ttcggcagcc
     4861 tcccgaatac gctccccctg ccctccctgc ccagcattga ctggagtacc ctaggggcac
     4921 tgacggcccc tgctttcacc atcgcgctgc tggcggcgat cgaatccctg ttaagtgcct
     4981 ccgtggcaga cggcatgacc ggagggcgcc acaagcccaa catggagctg attgcacaag
     5041 gcatcggcaa catcggctcc gccctgtttg gcggcattcc ggccaccgga gccattgccc
     5101 gcaccgccac taacatcaag gctggagcta aaagcccggt ttccggcatg attcacgccc
     5161 tgaccctgct ggccattctg atggcctttg cccactatgc ccagcagatt cccctggctg
     5221 ttctggcggg cattctgacg gtagtatgct acaacatgag tgaaatacac acgttcagcc
     5281 gtctgctgaa agggcccagg caggatgcgg cggtgctggt aatcaccttc ctgctgaccg
     5341 tgtttgtgga cctcgttgta gccgtggaag taggcgtggt gctggccgcc ctgctcttca
     5401 tgggccgcat ggcccaaatc agcgatgttt ccgccatcaa aaacgaactg ctggaaaatg
     5461 atgaggaaga tgatggaaac cgctccgccg ccaagctgga catcccggaa ggtgtggaag
     5521 ttttcgacgt gaaaggtccc ttcttcttcg gtgccgtgga gcaattcaag gaccaggttc
     5581 tggaaacgct ggaacatgat accaaggtgg ttatcctgcg catgcgcctg gttcccgcgc
     5641 tggacgccac cggcctgaac gtcctttccg acttctgcca ccagtgccgg gaacacggtt
     5701 ccaccctgct ggtttgcggc gtgcagcccc agcctctgga cgtcatccgc cacgcgccct
     5761 tttaccggga gctgaaacgc tacaatatct gcgagaatat tgacgccgcc ctgaaccggg
     5821 cccgcaaaat catcaacggc cccgcgccca aacacctgta acggcggccg tcaccccgtg
     5881 cggtcaggga gcaagacctc actctgcctt attcacggcc ttccggtacg ggtgaggctg
     5941 ctcctgctgc aaacagccag aaataacaac ctcggggaaa atgagggaag gccccttgca
     6001 ttccctcatc cggctcatct ccgggaatca ggcggcacaa tacggcctgt taactcttat
     6061 tgggccttcc ggcaaaacag gatgcccgag catggatcaa cgggccccgc aagcccgttc
     6121 ccggtaaaat gaaatagcct cgcatgctca cgaaaagcag aaacgtgccg cactccgaac
     6181 cggctccgtc agtcgggaaa gaggaacctg ttgaattgcg cagctgaata ccccaatacg
     6241 gcacggataa gtcccgttaa caatccgccg cctgctcctc atagcgggaa agaagaaagt
     6301 ccaggtcttc cagcagagac tggcaggaac ggtcccagcg cttccttccc cggagagcca
     6361 tcacgcattc cttcctgcct gtgaagcaat acctggtaat gccatgcccc cgcaattcct
     6421 ccacctgctt tttcagctcg caggcaaaaa acagccagaa cggcacgctg cgggcaatct
     6481 ccgcgacgca gaccgtcatg ggcggcatct cctccctaag ctcccttgca aggtgttcca
     6541 ggcccacttc cgtgagcatg cggcgcagac gcgcatacag ctcggacacc ttcatgacgg
     6601 acaaatcgca ctcatgctcc agataataac gcacggaagc cagcaggtct tccttgaacg
     6661 gaagggttgt tccggcacgc cgggcagcgt gctccaaagc atccgccagc cattgttccc
     6721 cgtactcggt aatcaggcaa tcaccaagct tgagcaacgg tctgttgttt cggaagccga
     6781 tcatggaaca gaaaatttaa agaatgggaa gcttgcgctg caggggatca ataagcgtct
     6841 ggtggcgcat ctgcgtaatc tcctggataa actcctccac attggaaaac tggcggtaca
     6901 cggacacata gcggatataa gccacgggat ctatggcata caatttttca atcaccttct
     6961 tgccaatctc gctggaaggg acttcgcgca aatggtcgcg gtgcaattcg gaaatgatct
     7021 cttccacggc gcggtccagg cgatccatgc tgacgggcct tttttcacac gcctttacca
     7081 ggccgcgtaa aatcttttca cgattcaggg cttctctcag attatccctt ttgactacgc
     7141 gcaactccgt gcgttcaatc tgctcatagg tcgtataacg gtaatcgcac ctcaaacaca
     7201 cgcggcggcg gcgaatggtc gtcccgtctt tggacatacg ggagtcgatg accttatctt
     7261 ccaaatgacc gcactgaacg catctcatgg cgcgaccata ctactacata acgcatccat
     7321 aaaggaaaaa gtacgccaaa tatagtacag tagacttgat gatcaatgct tcccaagcag
     7381 aacttcctgt gaataaaccg gggtaaaaat gccgcaggtc atgcccctgt tcggtttcca
     7441 gaccgctccg ctcctgtgaa taagaaaaaa gcccatggcc cttccgggag gaaaaaacgt
     7501 attttttaac ctcctccctc cactaaaaaa tacggcaagc attccggacc gtaccaggtc
     7561 tattgattac ggattcacca tgccgccttg attaacgaca ctgcccgcaa caggtctgga
     7621 gccattcaga ttcagcacag cgccctgctt gacgaagact ttgcccaaat tcacggcctt
     7681 cccctccata cccttcaaag tcatctccac ctttccggac aacgtccagg aacatttcag
     7741 ggcagagggc agcacctgtt ccaatatgac gcgggccgct tccgccgttt ctccgccgcc
     7801 ttccgcttgg gttacatgca cctccgcctg gccggacaaa cggacaggca tggctttttc
     7861 ctgaaccggc gagggcttaa tctccacggc agaaccaccc ggaatattcc aggactgccg
     7921 gcccgccagc tgtacattcg tcctcaactg caaatttttc ccctccgcca ggcgaatacc
     7981 tcccggcccg attaccaaac ggtctccgga acttcccttg ctccggacga taacagtctt
     8041 gacggccgga tcctccacac ggattccaaa caacttcacc acccctgaca ccaggctggg
     8101 ttttacggaa gaagccacat tccacacgca tacgttcttc cccggcattt tccggttcat
     8161 ccacgcgtcc ggagaaccca ggggaagatc gtaacgaccg cttcgcacgg agccggtatc
     8221 cttcccggcc agccgggcgg cttcataagc aaaacgggct cctatgctga tctgagactg
     8281 gccgctgtaa tgcactccca tttgatctcc tgcggtcagt ttgggaagat ctcgggtagg
     8341 ggcatagccc agggatggac gggattccgc cagatccttc atcacctggc gggttgtctt
     8401 tccctgaact tccgtatcgc gtccttccag gccgccggta aggttacccg tttccggatc
     8461 agattcattc tttccggccc aattggcatt ttctccaagg acggctttct gcgcggccag
     8521 tgcagatgta tccccgtacg gtttcagatc tgccgccaga ttgcccagaa gttccagaaa
     8581 gcgggaagcg ctctccggaa cggaagttcc ggcattgctt tccccctgca aatacaaaag
     8641 cccggcaaac tccactttgg aatacttgga cctgtccaca cccgcacagg cattcttgac
     8701 ggcctgcacc agcgtcctgt aagcctggcc gtttttccgc caatgggaat tgtcgccgcc
     8761 gtcccgggaa gctttcacga ccgcaacgtc cgcatccttg aaccatccat tcttctccag
     8821 agtaaaggca aacccgtatt ccggccccat gcataaattg ccgttataac gcggcatagc
     8881 aggccttacc tgctcccagg atgtggaagc gccgggaaac acgccttccc tttgcccaaa
     8941 attttcatgc cagtacagag tctccttcgg ttcatacttt tttaacagct ccggagaagc
     9001 agcgctgccc ttcaccgccc ccagggaatt agattggccc gtcagcaaaa aaatcttcaa
     9061 ttcttttgca gaacacacgg aaaaaaaaca caggaaaaat aaaaaggata gaaaacgaat
     9121 catttgaatt gcctttgcct agggacgact atagctgccg gaattttctt tttcaaccgt
     9181 caactattac ccataggagc atgaacaggg agatgtagga aaagaccagc agtcttaaaa
     9241 ccccattcaa agttttccga ggaatcatta ggaaaaagaa aacccggaag aatcattttc
     9301 tcccgggttt cttcaaacaa ttttctgaaa ataatgacgc agaggcgcat gggcagtttt
     9361 ttgcttccct ttcccacctc tatcaggctt cctgccttct gcgcctccgc atggccagtc
     9421 ccgccaaacc cagcaggctc cgggaagccg tcgccggttc cgggaccaaa tacgcagaca
     9481 tggcagcctc atgaagtata gcttcctacg gacaactcct gaacgttcga ggaggatagc
     9541 aacagatcac ataaccaaac ttaaagtgga aaatatgacc gagcaagaaa agaacaggcc
     9601 ccgcgagtca agtgactggc ggggcctgat ggaaaaaaat cccggcggcg acctactctt
     9661 gcgggacctg tcgtccgact accattggcg cggcagcgtt tcacttccgg gttcggaacg
     9721 ggaccgggtg ggaccactgc gctctggcca ccgggcttca gggggaagag gtgatttttt
     9781 cttcatcctg cctgcccggg agccctgctt ttttgggggc ttctgagtga gggatttttt
     9841 tcagggtatg tgtgtggcgg ctaagaagat ttccgtcctt tgtgtgggga gggattcagc
     9901 gtaaactgac atctgcgtgg ctggtggttg atgaacatgc acaggtaaat gagacaagag
     9961 caagttgtca ttcacgacgg ggagtgatgt aatatgatga agtcaattgg taattagtat
    10021 cgctcggctc aatacattgc tgcacttaca cctgcgacct atcaacgtgg tggtcttcca
    10081 cgaacctgta gggaaaactc atcttgggaa gggcttggcg cttagatgct ttcagcgctt
    10141 atcccgtccg cacttagcta ctcggccatg cacttgacag tacaaccgat gcaccagtgg
    10201 tgcgtcagac ccggtcctct cgtactaagg tctgaacccc tcaattttcc aacgcccaca
    10261 gaggatagag gaccgaactg tctcgcgacg ttctgaaccc agctcgcgtg ccgctttaac
    10321 cggcgaacag ccggaccctt gggaccttct ccagccccag gatgcgacga gccgacatcg
    10381 aggtgccaaa ccacgccgtc gatatgaact cttgggcgtg atcagcctgt tatccctaag
    10441 gtacctttta tccgttgagc gacggcaatt ccactttcta ccgccggatc acttgggcct
    10501 gctttcgcat ctgctcgact tgttggtctc acagttaggc gggcttatgc ccatgcactc
    10561 gacacccggt tgccaaccgg gctgagccca ccttcgcgct cctccgttac tctttgggag
    10621 gataccgccc cagtaaaact gaccggctga cacggtcctc tggccggatt cacggctcag
    10681 ggttagatcc tccagtttca aagggtggtg tctcattgat gactcggacg ccccctaaag
    10741 ggcgtcttca gcgtctccca cttactctga gcattaaaac cgaagaaaca atgacagctt
    10801 acagttaagg tctatagggt ctttccgtcc ttctgcgggt aggcggcatc ttcaccgcca
    10861 ctacaatttc actgagcgcc tggttgagac agtgcccaac tcgtttcacg attcgtgcag
    10921 gtcggaactt acccgacaag gaatttcgct accttaggac cgttatagtt acggccgaca
    10981 ttcaccggga cttgggttta gagcttcgcc ttgcggctaa ccccttgcct taatctttcg
    11041 gcattggtca cgtgtcacac cctatacttc gacttgcgtc tttgcagagt gctgtgtttt
    11101 tgataaacag tcggttgggc catttctctg cggcctgcat cgctgcaggc accccttctt
    11161 ccgaagttac ggggctaatt tgccgagttc cttaaccagg tttcactctt acgccttggt
    11221 atattctacc cacccacctg tgtcggtttg cggtacgggt cgcttagcat acgctggggc
    11281 ttttcttggc actcgatcca atgatgcgct gagtccgtgg actcaactcg gaattcaata
    11341 acctagtcat cttttctcat gcgtcccccc agtttaaggg ttgcgagctc aggattatta
    11401 acctgatgtc catcgcctac gcctctcggc ctcggcttag gtcccggcta acccagggac
    11461 gaaacacgtt gccctggaaa cctcgggttt acggcggccg gggatttcac ccggcttaac
    11521 gttactcatg tctgcatact cacttgcatg tgctccaggg tcagtcgcca tcgcccttcg
    11581 acgcgcatgc aacgctcttc taccactctg ccttgcggca aagtccagag cttcggtata
    11641 acgcttgatc gccaatcatt ttcggcgcag gatcactcga tgagtaagct attacgcatt
    11701 ttttaaatgg tggctgcttc taagccaaca tcctcactgt ctgtgtaatc ccacatcctt
    11761 tccactgagc gttatttagg caccttagct gctggtctgg gttgtttccc tctcgacaat
    11821 gaagcttatc ccccactgtc tcactgccac gttccattgc acggtattcg gagtttgata
    11881 aggtttggta agcgggtgtg ccccctagcc ttgtcagtgc tctacccccg cgcatcagca
    11941 cgtgacgctg cacctaaatg catttcgaag agaaccagct atcacggggt ttgattagcc
    12001 tttcactcct accctcagct catcagagaa cttttcaacg ttcacctgtt cggtcctcca
    12061 catggtttta cccatgcttc aacctggcca agggtaggtc acctccgctt cgggtccagt
    12121 acctgcgact tattgcgccc tgttcggact tgctttcgct gcgactccgg gccggaagcc
    12181 cttagtcttg ccacaaacac tgactcgcag actcattaaa caaaaggcac gccatcgcag
    12241 attgctctgc tctgacactt tgtagacata cggtttcagg ttctatttca ctccgctcac
    12301 agcggttctt ttcacctttc cctcgcggta cttgttcact atcggtcgcc aactagtatt
    12361 tagccttgcg cggtggtccg cgcgggttca tacatcgttc cacgtgtgat gtattactca
    12421 ggataccact aattcccact ctggatttcg tctacagggc tttcaccttc tccggccagc
    12481 ctttccaggc tgttctacta tccagtatgg tcgaatatga cgtggtccta caaccccgaa
    12541 ggaatacctt cggtttgggc tccatccgct ttcgctcgcc actacttacg gaatcgattt
    12601 ctctttcttt tcctccgctt actgagatgt ttcacttcag cgggtttcgc gtttcatacc
    12661 ctatgtattc aggtatgaac gatcgagtat tgcctcgatc aggttacccc attcggatat
    12721 ccccggatca tcgcttgctt gcagcttccc gaggcttttc gcagcttgcc gcgtccttct
    12781 tcgcctgttg gcaccaaggc attcaccgta cgcccttact tacttcatca tattgcaaaa
    12841 gctgttacca cttccacaat accacaaagc cctttcgtga cttactgtat tgtctcattg
    12901 cttggtatgc atgtttatct tgtgttttgt agttgttgtt caactaccca ctttgttgga
    12961 aagcgattgc ttctcagcaa tcgttttctt tgccacgcag atgtcaattt tcgctttccc
    13021 tcaataatcg attttgttgg tgggcctgac aggacttgaa cctgtgaccc cgcgcttatc
    13081 aagcgcgtgc tctaaccaac tgagctacag gcccggcttt gggtctcttt cccgaaactg
    13141 gtggaggcac ggggactcga accccggaca tccagcttgc aaagctggcg ctctaccaac
    13201 tgagctatac ccccagttgc ggttctcggt gaaaaaaggt cgtcacgcac gtcgtgccag
    13261 gtttcgctcc aggcgtctgg ctgtttacac atctttggtt cgttgcgggc cctcgcgggt
    13321 tccgctttct ctcccctcgg gaagagatcc gacctgtgca cttccgtgca cttgctccat
    13381 agaaaggagg tgatccagcc gcaggttccc ctacggctac cttgttacga cttcatccca
    13441 gttaccagtc tcaccttagg accctgcctc cttgcggttg gcttcagata cttcgggtgc
    13501 gaccggcttc catgatgtga cgggcggtgt gtacaagacc cgggaacgta ttcacggcgc
    13561 cgtagctgat gcgccattac tagcgattcc ggcttcgtgt aggcgggttg cagcctacag
    13621 tccgaactgg gcccagtttt taggatttcc tccgcctcgc ggcttcggcc ccctctgtac
    13681 tgggcattgt agtacgtgtg cagccctggg cataagggcc atactgacct gacgtcgtcc
    13741 ccaccttcct cccagttgat ctgggcagtc tcgccagagt ccccaccttc acgtgctggt
    13801 aactggcaac aggggttgcg ctcgttgctg gacttaacca aacatctcac gacacgagct
    13861 gacgacggcc atgcagcacc tgtgtaacgc ctccgaagag tcgcatgctt tcacatgttg
    13921 ttcattacat gtcaagccca ggtaaggttc ttcgcgttgc atcgaattaa gccacatact
    13981 ccaccgcttg tgcgggtccc cgtcaatttc tttgagtttt aatcttgcga ccgtactccc
    14041 caggcggcac gcttaacgcg ttagctccgg cacgcagggg gtcgattccc cgcacaccaa
    14101 gcgtgcaccg tttactgcca ggactacagg ggtatctaat ccctttcgct cccctggcct
    14161 tcgtgcctca gcgtcagtta atgtccagga acccgccttc gccacgagtg ttcctctcga
    14221 tatctacgca tttcactgct acaccgagaa ttccggttcc ccctccatta ctctagtctc
    14281 gcagtatcat gtgccgtccg cgggttgagc ccgcgccttt cacacacgac ttacgaaaca
    14341 gcctacgcac gctttacgcc cagtgattcc gaacaacgct tgagacctct gtattaccgc
    14401 ggctgctggc acagagttag ccgtctcttc ctcttgtggt actatctttt taatttgctc
    14461 ccacatgaca ggggtttaca atccgaagac cttcattccc ccacgcggcg tcgcaccatc
    14521 agggtttccc ccattgtgaa tgattctcga ctgctgccac ccgtaggtgt ctggaccgtg
    14581 tctcagttcc agtgtggccg gacatcctct cagaccggct acccgtcatc gccttggtga
    14641 gccgttacct caccaactaa ctaataggcc gcgagcccat ccccaagcgc attgctgctt
    14701 taatctttcg atactatgcg gtattaatcc cagtttccca gggctatccc gctctcgggg
    14761 gcaggttact cacgtgttac tcacccgtgc gccactagag aattattagc aagctagcaa
    14821 ttctctcgtt cgacttgcat gtcttatcca cgccgccagc gttcgttctg agccagaatc
    14881 aaactctcca tcaaattaat ttttgaaatt gctggaccaa atggaatctt gcgatttcat
    14941 ttgcgccatg cttcagccag ctcttccttc aagttgtgtc tcttgaaggc ctcttcgctc
    15001 cgcctcgcac ggcgcgcgtc acgacctttt ttctttcccc ttgcttctac caaaccaccc
    15061 ctctcagagc taggtcgctc cttcgggctt ttacccctca gacacctctc ggcaccctcg
    15121 gaaaaccctc ggcggtttcg gtagtcattc ccttgaaatc ttcttatcgc aaccccttcc
    15181 ggggtttttt ccgggtggcc ggccccagcc gcttccagca ctcttccgag cccttcagcc
    15241 gccgggcttt tcatcccgtc gccgtgttgg cgtgccgaga aatctatcat accacacctt
    15301 tttgtcaatt tttttcgtgc tgttttttgc g
//
LOCUS       DAWEMX010000053        15050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1643_length_15050_cov_13.012337, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000053 DAWEMX010000000
VERSION     DAWEMX010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1643_length_15050_cov_13.012337"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5488..5497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8856..8865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3272 a         4413 c         4275 g         3070 t
ORIGIN      
        1 gccccttctg gaacagaagc acgcgggtat tgcccggtcc cgtatggacc acaagcactt
       61 ttttcttgct gaaccccgga tactgggcca gagcctcctg caccttcacg taaatgaggc
      121 gcgtcatctt gccgtcgtca atgcggcgcc cccgcaagcc gtgggccacg tgcatgcggt
      181 tcacgaacac gtccacatta tccgcctcgg aaatgatgtt ggaaatcatg aaccggacgg
      241 aaagcttgct gccggctccg tattccttga gaatttccag atagtcgccg atgatttgca
      301 cgcagcggtc catggtatgc cgggaaatgc ggtggaaccg gaaaatgtca cgcgccatcg
      361 gaacgggctg ctgaaggaaa tccagcaggc gaatcctgtc ccggacagcc tcagccacca
      421 tcaggctcat ggagctcggg cccagataga tcaccgcctg agttgataat tccgaatcct
      481 gaacctgctc gccttgaatc attgccatac catgtattgt ttcccggccg tggagcaaga
      541 acggcaacgt gacaaaaaag ccgcttccgc cccttccagc aaaggaaaga ataaagaatt
      601 tgccttttga cggcaagaga taagcgcggc tcccttcccg tcccggcgga acggctcttc
      661 aagacggtta tttcccatag gcccccagca ggggaaccag ccgcggggca aatcttttca
      721 tgcagaaaaa aagccccgcc agcacggcga aaatgaccgg aaccagaaag agccagagca
      781 tgtcccaggc ctcccccttc catgtaatcc agccgctttc acgcagcagg atgatgacga
      841 tgtaatgcgt tccgaaaatg aaaaaacatg ccggagccag ctccgccacc cacctggcag
      901 cgcgcggaaa ccattccgtg aacagaatgg ccagggacat cagccccagc atgcccaggg
      961 cgatgagcat ggagttgtgc tggtcccggt ggcctgccag ccaccaggta agcgcaagcg
     1021 tggcgaccag gaagggcacc gcgtaccggc gcacggattc cgtcaggaac gggagggaat
     1081 accgggaaac atacattccc agcaggaaaa atcccaggct gttggcgatg ggataatcat
     1141 gatccgccag atccagcgca aaatagttca gggacagcat gaccatgccc gcccagagca
     1201 gatagtcccc caaacggtaa agaagggggg ccgcagcggt atagatgatg atatcgcgca
     1261 gaaaccacat gggagcgtcc accggatgcc cggcaatgcc caggcagcgg ccgaacgccg
     1321 cgagagcagg ccagaacccg gctccctttt cccctcccag ccattcatac ataggccggt
     1381 aggacgcgta atattcgtgc aggggggacc agccgatcag aacggccgca gccagtcccc
     1441 acaccaggta cggacgaaga agaaaccagc tccgccgcca gaggaaaagg ctcccgccat
     1501 ccagagggcc aaaagaccct cttcccacaa aatagcccgc cgccagcaga aaaaagggcg
     1561 tgcgcgccgc caggaactcc gcccatatcc accggtacag ctccgccgaa tagaaagcca
     1621 tgtgattcgc aataatcagg atgcaggcgg ccacgcgggc cacatccacc cacaccatcc
     1681 ggcgggcggc ggtcttctcc agaatcacgg gaacggaggc ttccggcggc gtcattccct
     1741 ggagaggctg aagttacaaa ggagcaatgc cgagctgctc cggcaacgcg atgcgggggc
     1801 ggtccgtcac aatcaggggc gcgccgtccg ttacgataac cgtgtgctca aagtgggcgg
     1861 aaggcttgcc gtccgccgtg acgaccgtcc agccgtcatc cagaatttta acggaaggcc
     1921 tgcccgcatt caccatcggc tcaatggcca gggccatccc ctttttcagg cgcggaagct
     1981 tgtaatgggg gcggtaattg ggaatctggg gttcttcatg caggtggcgg cctatgccat
     2041 gccccacaaa atcccgaacc acagtaaaac cgcggggacg cacgaagccc tccaccgccg
     2101 cgcagacgtc cgccagcaag gcgcccggcc ttaccagttc aatggcgcgg aacagggatt
     2161 cctccgtcac ggccagcagc cgcagttttt ccggatccac catgcccacc ggaacggtca
     2221 tggcgttgtc tccatgccag ccgtccacaa tggcgcccac gtccaggctc acaatgtccc
     2281 cgttctggat gcggcggggg ccgccgctgc cgtgcaccac ctcttcattg atggaaatgc
     2341 agagctggcc gggaaacccg cggtaccggt aaaaggaatt gcctaccttg tgctttttaa
     2401 aaatttcacg cgcgatatca tctatttccc gcgtggtgcg tcccacctgg acttcggccc
     2461 cgatctccat caagatttcc gccgttacag ccccggcttt ctgcatcttg gctacctcgc
     2521 cgggagactt gatgtggatt cgttctgcca tgggattatt ctttctattg ccggtactct
     2581 tccagcttgc gggccagatc ccgggaaaca tcggcaggtt cccgttcttc ggacaccgtc
     2641 attttaacga ccaggccgcg ggcctcgtag tacctggcta cgggcagcgt catggcttca
     2701 aaatccttcc agcggcgggc gaaagcttcc atgttgtcat ccttgcggga aaccattttc
     2761 cctccgcact tggaacattc ctcttccggc gtctgcacca cgtaaccgca tgtggaacat
     2821 tcccgacggc gcatgatgcg ctgttcaatg agttccctgg acacatccat ccagacgact
     2881 acatccacgg aggttccccg ctggtttaga aaatggtcca gcgcttccgc ctgcgcaacc
     2941 gtgcggggat agccgtccag cagccacgcg ccatggtcca tctccgacag ccagccgcgc
     3001 accatgtcat tgacaatttc gtccggaacc agcatggccc tgtccatata gctcaaggct
     3061 ttccggccca gttccgtgca ggattccact tccctccgca acagggagcc ggtgctcagg
     3121 gagtgaaggc cgaaggtatc agccaaaaac cggccctggg tccctttgcc agatgcggga
     3181 gccccaacca gaatgaaatg cttgagttcc ttcatatctt tactttcagt aaaaaagggg
     3241 gcttactgca tggcgttgta ggcgatggct ccggccagca caagcagggc gataacaatc
     3301 cacatgacgg taacggtgcg gctgcccgcg gcggcgccgg taccttggat gtgcccgtat
     3361 ttgcccttga gcttgccttt tttcaggaag ccgtcgtaat tgcgggaaag aagggtcgtt
     3421 tccacctggc gcatcatgtc aagcagtacg cccaccagaa tgagaaggct ggtgcctccg
     3481 aagaactggg taaccaggta ggggatattg cccccgatat tcagcaggga aggcaggaag
     3541 taaatgaggg tcaggaatgc ggacccggca aaggtcagcc tttgcatggt ctggtccagg
     3601 aagagggcgg tgggggggcc gggacggatg ccgggaatat agccgccatt gcgcttcagg
     3661 tcttccgcga tctgggaggg ctggaacatg gtcgccaccc agaagtagga gaagaagaaa
     3721 atcatgaggc cggaaataat gtaatagccg gaagaagcgg gattcagcca gtcattcatc
     3781 ttggtcgcga tccactgcag cgtagtgttg tagtgggcga tctgggagaa gagaacctgc
     3841 ggaagggaga ggatggccgt agcgaaaatg acgggcatca cgccggagta gttaagcttg
     3901 agaggcaggt actgcgtggc accgttgaac attttattgc ccatgacccg cttggcgtac
     3961 tgcacgggaa tgcgccgctg ggcctgcgtc accgtcacga cgagcgccac cacgacaatc
     4021 aggaaggcaa tcagggccac gagcagcatg gctcccatgg ggacaaccgc gccgtccttg
     4081 tacaccaggg ttttccaggc cagggtgact gctcccggaa gggcgtgaat aatgttgacg
     4141 gaaatgatga gggaaacgcc gttgccgacg ccgcgttccg taatctggtc gcctatccac
     4201 atcaggaaca tggtgcccgc caccataatg agcaccgtca tgagggcgaa ctggatgccg
     4261 gggtcaatga caagctggca gtccgcggga atgttgagac ccacggcctg caggcgttcc
     4321 gggtgttcca gcgtgccgac caggaaccag ccctgcacaa gggcaatgac gatggcgatg
     4381 gcgcgcgtcc acttggtcat tttctggcgg ccgccttctt cccgcaccat cttggcccac
     4441 tggggaacca ccgcggaaag aagctgggtc atgatggaag cggaaatgta gggcatgatg
     4501 cccagagcaa aaatgccgca ctgctggagg ccgccgccgg agaaaatggt cagcacggta
     4561 gccagacccg ctccgggacc gctggcgttc gcggaggctt caatcacttt ctgcagctcc
     4621 atgacgtcaa tgcccggcag gggaatctgt acccccaggc ggacgacgac aatcatcgcc
     4681 agtgtgaaca aaatccggcg cctgagctcc ggtactttca tggagtttgc aaatgcggat
     4741 atcatggtcc tgtaatataa aagttggcgc gcagcttggc ttgcatgtcg cggcggtttg
     4801 cacccggggc atccatggga accgcaacag taaacacaat gatgcgaacc gggtcaagct
     4861 gcatgacccg gttcgccaaa tgtgattagg cttcgctaag cacggtgacg gtgccaccgg
     4921 cctgggtcac cttctcgcgg gcactggcgc tcacaaagtc cacaactacg ttcaggttct
     4981 tggtcagggt gccgttgcca agaagcttca cggcatcgca gggaccttgg acgagcttgg
     5041 ccgcacgcag ggtgttttca tcaaccgtag caccggcttc aaacacgcgt tcaagctggg
     5101 agaggttgac gataaggatt ttatcctgga agcgggtgtt gttgaacccc ttcttgggca
     5161 gacggcggtg aaggggcatc tggccgcctt caaaacccgg ccggatcgtg ccgccggaac
     5221 gggccttctg gcctttgttg cctttgccgc aggtcttgcc gagaccggag ctttccccgt
     5281 tgccgaggcg cttcttgcgg tgcttggctc cgggattggg cttaatcgta tgaagctgta
     5341 acatgttatt tacaatggtt ggatttcttg gaggcggcgg cgccgggctg ccgcctccgg
     5401 atcagattag atggcctttt ccttcttctt gccgcggtcg gcaagaacct ggtcgctcgt
     5461 gcgcagggaa gcgagagcct gcagggtnnn nnnnnnncgt tggcgtggtt ggaggaaccg
     5521 agggatttgg caacaatgtc acgcacgccc gctgcttcca gcacggcgcg gatggtgttg
     5581 ccggcaatca ggccggtacc gggggaggcg ggcttcagga gcaccttgcc gccaccgaat
     5641 tcggagtaga cttcatgcgg aatcgtgccg ttggcgatgg agaccgggcg aagctggcgc
     5701 ttggcggctt ccgtagcctt gcggatggcg tctgccactt cattagcctt gccgaagccg
     5761 aagcctacgc ggccttccat gtctccgctc acgacgaggg cggagaagga gaagcggcgg
     5821 ccacccttga cgaccttggc gcaacggttg atgaacacaa ccttttcagc gagctggggg
     5881 ccttcttcca cgggagcttc agcctgaggg ccacggcgtc cgtcacggcg gggtccacgg
     5941 ggaccgcggc cattctggcc gtcacggcgg ggtccgcggg gtccgcggcc gcccgttgcg
     6001 ggttgctgcg cttgagcggg agcggcggct tccgtagcag gagcagtgtt ttccgtttcg
     6061 ttattcattt aaggaatctt tcaattagaa tttcaaacca gtttcgcggg cggcatcggc
     6121 cagggccttc accttgccgc agaacttgaa tccaccgcgg tcgaaaacga cttcagtgac
     6181 attggcggcc ttcgcgcgtt cgccgatcag attgcccacc ttggtagcgg tagcgcagtt
     6241 ggcgagtttg gaaggatcgg cgatggaagc atcggccgtg gaagcggagc agatagtcct
     6301 gcccaccgta tcgtcgatga tctgggcata gacgtggcgg ttggagaaat agacagccag
     6361 gcggggacgg gaagccgttc cggaaacctt tctgcggatg cggcggcgaa ccttggcgcg
     6421 gatggctttg cgattgatag tactcatcgt gtataaatat gttgtaatag ttattacctg
     6481 aattacttac cgacgctctt gccttccttg cggcggataa cttcaccgac gaaacgcaca
     6541 cccttgccct tgtagggttc cgggggatag tacgcacgga cttcagcggc aaactggcct
     6601 acgacctgct tgtcgatgcc ttcgaccgtc actttcgtgt tttcagtcac ggttaccttg
     6661 agaccttccg ggattacctg gttgatcgga tgggacttgc ccaggttcag atccagaatg
     6721 ttgcccttga cggcagcacg gaaaccgacg ccgacgattt caaggttctt gacgaagcct
     6781 tcggaaacac cgatgaccat gttgttcaac agggaacggt tcgtaccgtg cagggcgcga
     6841 agctggcggc tttcgccggc gcggtcaaca gagagctgat tgccttcaac ggtagcggta
     6901 atgccttccg gaagcgtcca ggaaagcttg cctttggggc cttccacctg aacggaagag
     6961 ccgttaacct ttaccgttac cttgtccggc aatgtgatgg attttttacc aactcgggac
     7021 atatgtttag gaaatgttaa tggttaccat acaagggcga gaagttcgcc gccgatgctc
     7081 tgcttgcgcg cctgcgcacc ggtcatcaca ccgcgggagg tggagacgat ggcaatgcca
     7141 agcccgctga gaacgcgggg aatgtcctcg gaagaaacgt actggcggcg gcccggcttg
     7201 gagctgcgct tcacatcggt aacgaccggc ttgccctgcg gggtaaactt gatcttcacc
     7261 ttgatttgct tcttggagcc ttctccggtc acttcatagt tccagatgta gccttcttcc
     7321 tgcagaatcc gggcgatttc caccttgatg ttggagcagg gagcggtaaa ttcctcgttg
     7381 ccggccaggc tggcgttttt caggcgggtg aggaagtcgg aaatgggatc acttaatacg
     7441 gccatggtaa gaaatagtta ggcgttggcg gtttgggttt cttcggcctt cttcggttcg
     7501 cgcatgggga cgcccagagc gcgaagcagg tcgcggcctt catcgtccgt aggagcggaa
     7561 gtcacgaaaa tgatatcgaa gccgatctga cgcttgatct ggtcaagttc gatttcgggg
     7621 aagatggact ggtcggagat gccgacggca tagtttccgt tgccgtcaaa ggacttgtag
     7681 ggaagaccgc ggaagtcgcg aatgttcgga gccgtaatat tgatgaagcg gtccaggaat
     7741 tcccacatgc gggcgccgcg cagcgttacg cgggctccca gaggttcgcc ttcacgcacc
     7801 ttgaagttgg ccacgctctt gcgggcatag gtgacggagg gcttttgtcc ggtgattttg
     7861 gcgatttcat taacggcatc ttccacggcc tgcttgcggt ccgggtgctt gcccatgcag
     7921 gaggtgacaa caatcttctc caggcgagga atctgatgca cgttcttgta tgcatgcttc
     7981 gcttgaaggc cgggaacgac cttttcacgg tagtatgttt gcagtgttgg tgtactcatt
     8041 gttttagcgg gtcagctctt tattaatgat tgatatggct taagcgcagg gtcagaccgg
     8101 atggcctagc ctactttttt cacgttggag atgtgaatcg ggccgttgat ttcctgaatg
     8161 ccgccttccg ggttctgttc ggtcgggcgc acagcctttt tcagggtgcg ggcgccttct
     8221 acgatgacag tacctttgga agcattcacg gcgagaacgg tgccgcgctt gcccttgtgg
     8281 ttacgtccgg cgatgatttc cacttcatca ccttttttca cgtgggtctt catatatgac
     8341 tatttcttac gcgtttatgc gattaagatc taccaccccc ttggtggcag ggcaggaaga
     8401 gtattatagg atagaccttt tcgtcaagcc cgcatcctaa aaaaaatcgt tttaccgccc
     8461 ctgcgccaat gcggaacgat aaatgggaaa ccgttcccgg atgcaggaat tccgtagtga
     8521 ggggacagtc ctccggggga ctgggcttcc gcaacggcct attcatcgtc cgaggggatt
     8581 tccgccccct gccaccgcag cttggccctc agcagggcgc cgaaattctg cttccggaga
     8641 tgaaccaggg aaagcgtttc cggcgccttc cggattacca ggctttctcc cacttcgatg
     8701 ggataggcgg aacggccgtc tagggaatag accatggagt caaagcggcc gcggcgctcg
     8761 cggggccgca ggcgtatggt catgttgtca ggaagcacca cggaacgatt ggtcaggctg
     8821 tgcgggcaga tgggggtgac gcatacaaca cgggannnnn nnnnngccag accagaggcc
     8881 ccccggcgga aagggagtat gccgtggagc cggtgggggt ggaaaccaga acgccgtccg
     8941 catggtagcg gttcagcaat tctccgtcta tctccgcatc caggtccacc atcttgccgg
     9001 actgggcgcg gatcagggaa acttcattca aggccagctt gcgcggaggt gccgcaacgc
     9061 cgtctccccc cacccggacc acttcaagca tggaacgttc atccgtcagg taggaacctt
     9121 cctgaagggc atacgccaga agcggcagtt cctgcacgga acaggtggtc atgaacccca
     9181 gccggcccag gttcactccc gccaggggaa cgcggtgcat ggccgccagg gaagagacgg
     9241 tcagcatggt accgtcccct ccgaaggtca ccagcatgcc gcaccgtccg atagcccgtt
     9301 ccagagcatc cggcgtttca atgacgcagg agcccagccc gtggcgggaa agttcccggc
     9361 gcatgatgcg cagcgcttcc cccagtcccg gtttttccgc cagagcgacc aggccgatta
     9421 ccttggaagg attcatgcgg gagatcatac tgaatttttc cgggccgctc aactggtaaa
     9481 ccgcaaccgg gaaaagattg ttccgccctg ctctattcct taaatttcaa ctcttgttcc
     9541 ttttttgtta tacaaaacca tcaatatgtg ttaaacgtaa aaagcgtgaa atggttcctc
     9601 cccgtcctgt tgctttttct gatgccgggc tgctccacta cggagaagtg cgctcagaaa
     9661 acggagccat ccgttcagga ggcagaggcg catgtcaaaa atgcgcccta ccgggtgcgt
     9721 ggaaaaaggt atactcccat gagcgtggcg gatgccctgc aataccatga gaccggctat
     9781 gcctcctggt atggcggcaa agcgcgccgc ctgaagacat cttccggaga atacatcaat
     9841 ccccagcgtt ccatgacggc ggcccacaaa accctgccga tgccgtgcaa ggtgaaagtc
     9901 acttgcctgg aaaccggcaa atcaaccgtc gtcaggatca acaaccgcgg gccttttcac
     9961 agcaaccgcc tgatagacct caccaccgcc gccgcaagcc gcatcggcct gcatagacgc
    10021 ggcgtatcca gggtccgtct ggaagtcatc tccgtgggtg acggcccgca tgaggtttcc
    10081 gcccgttaat cccatctcat ccttttccgt atatttatca ttttaccctg catgattccg
    10141 ctccgcattg ttcttgcctc cctggcatgc ctgtacccgt tctctctggc tcaggagcct
    10201 aaatcggatc ccgataaaac gctgattatc atttctacca gcgatatcca cggcaatctg
    10261 gacaatttcc caagactggc cacactggta aagcaatacc gcgctaaata tccgcacgtg
    10321 ctgctggtgg attcaggtga ttatttcata ggcaatccct atgtggacga ctgtgaaaaa
    10381 cggggcgaac cccttactat cctgatgaac aagctggggt atgatgtagt gaccatcgga
    10441 aaccatgatc tggattacgg gcaggaggcc ctgcgcgacc atatcaaggg aatgccctcc
    10501 accaagtttg tcattaccaa cgccagcctt tcccctactc tggaaaactg tttttctccg
    10561 tatgtcagca ttccgattaa aggaacgtcc atttccgtag gggtcatagg actggcagac
    10621 cttcaaacca cggatgtgag gaggatgacc ggcatctcat ggaagctgcc ggatgaagaa
    10681 gactacaagg gaatcacgga caggttccgg ctccaccata ataccatcaa cgtcatcctc
    10741 agccatctgg gatacggcaa tgatctgaaa atgatgaagt attctccgaa tatcgacgtc
    10801 attcttggag gccataccca tgtcatgctg cccagcggtc atctcagaac cggaaccctg
    10861 ctcagccaca cgggacacag cctgagccat gtaggcgtta cggaaattat tttctccacg
    10921 gaacatcccg tctccatcct ttccaaatca accagggccg tttctttgaa tgaggaaatt
    10981 cccgatgacc cggaagtaaa ggaaattgtc cgccgatttt ccggaaatcc gcttttcaac
    11041 cagcaagtgg gcgtcgccgg agaagaaatc acgcacgtca ccataggcac ccttttctgc
    11101 aaggccattc agcaggcctc ccattcggat attgccattt acaaccgcgg cggagtacgg
    11161 tccaaaaacc atcttaacaa aggacccgtt accatcaggg acatttacga gctggaaccc
    11221 ttccgggaaa agatcgtcac ctgctccatg agtaaagcgg acattgaaca actgatcctg
    11281 tccaaattca tgtctccgac cgatgacgaa ggaggcgttc tggaagtgta ctgctccgga
    11341 ttttcctacc agatcatgga cggtgttacg cccagcatta ccagcacctt gaaaaacggg
    11401 gtcatttaca ctgttgcgat gggagactac ctgtgcagca atttcatatt tccccaacgg
    11461 gggaacggca aaccgacggg aatagatgtc cgcaaagccc tgattgatta cctgaaacaa
    11521 atcaaggagc tgctcaatcc tccgccgtcc aagctgccta tcatcaaaga tacgacgttc
    11581 gccctgtgaa ctacggctgc aagcacacgc tacattcccg ccagccatgc agccggatcc
    11641 ctatcatgca gtaccggaag agaaacggaa tatcaatggt ttccgcatcc tcccctccgg
    11701 tgctttggtg acggacacac cgcctttcga ccgtctgttt gaagaaggaa ccggaaacgg
    11761 gctgtatgcc ctggcggtat ccaactggga aaccgttccg gatccgggcg ccttctttat
    11821 caagaacatg gcgcgccagg cgcttacccg catggcgcac gctgtcgcag aaaggccgga
    11881 cgaggtggat accgttctgg accacctgct ggcctccgct acggaaaaag cctttctggc
    11941 ggagcaattt ccccccgtac cgggcgcgga atatgccact ccggacatca ttgccggatg
    12001 gtttgaagat ttgcggaagg caatagcaaa ggaaatccgc gtccgaggca ttacccccgc
    12061 cgaatggttg aatagcctgg gcgcgccctg gaaccagata ggcaaggtat ttttccacct
    12121 tgcggaaaac cgggatgacg ccacaggctc ccgccccttt gctttcatgg ctacctttgc
    12181 gcatcaaagc gctgcggaca accaggtacg ccacctcccc cttgccaccg ccctgaaact
    12241 tcatgaaggg aattacaccg ccctgctggc cttactccag cctttaaaag aagcggcacg
    12301 cgaaagcccc ctgctgaagc gcctgctgga taacgggaaa atttatacgc cgatggcctg
    12361 gagcgctccg gaggcccggg attttctgca ggacacgccg gtctacgaac gcgcaggcat
    12421 cattgtgcgc atggtgaatc tatggaaaaa aacccctccc aaactacagg taaaagttac
    12481 ggcagacgca tccggaagtg aaacaacggt ccattcttcc gggctttccg tccgcaggct
    12541 gctgaaattt tccgtatccg ccactctggg aggcaaagac ctgaccccgg aggagctggg
    12601 ggaactgctc agcaacggag acggcctgat ccgcttcaag ggggaatggg tgtgtgtgga
    12661 cgcccggaag gtccgggact taatggaccg gtggactcaa gcggcacgta tgatgagctc
    12721 cctcggcatc cctctggtcc agggtctgag aatgctggtt aacggccctt ccggccagct
    12781 tgtacagatt ccggaaccgg acgaagactg tgtcatggaa ccgggacaaa acctccggca
    12841 gaccctggac atcctggcgg ggaaagtccc agtctccata ccggagcttc cttcccggct
    12901 ggacgccctg atgcgccctt accagaaaga agggttctct ttcctttacc ggattacgga
    12961 acggggtttc ggcgcctgcc tggctgatga tatggggctg ggaaaaaccc ttcaggctat
    13021 tgtgtggctg gacgcattgc ggagaaaagg ccggctggag ggcctgccgg ctctgattgt
    13081 ggctccggcc tccctcctgt ccaactggaa agaggaagcg caacgcttcg ctccggagct
    13141 tatcctgcgc atcatgcacc cttcctcccc ggacccgtgg cagccggaaa ataccctgcg
    13201 ccggaaagac tgccacgccg tcatcactac gtatggcatg gctgcacgca cgtccgccct
    13261 ggccgacctg catttcccgg ccattattct ggatgaagcc caggctatta aaaacgcagg
    13321 ctccgcccgt tcccgcgcca tccgttccct gcatggggaa cgacgcatag ccctgtccgg
    13381 cacgccggtg gaaaataacc tgaatgaact ctggagcctg atggaatttc tgaatccggg
    13441 gctgctgggc agcagaaaaa gcttcgaatc cttcacccgt tccctggaac gcggttatgc
    13501 cccgctgcga cgcctggtgc gccccttcat cctgcgcaga atgaaaacgg atccggcgct
    13561 ggttcccgac ctgccggaca aaatggaaat accggcctat tgctccctca cgccggaaca
    13621 ggccgccttg taccaagcac agatagacat gctgcatgcc gtactggacg aaccggaccc
    13681 agctgcccgc ctgatgctga ttttacccat tctggcacgc ctcaagcaga tctgcaacca
    13741 ccccgcccag ttccagggca cggacgatta tgcgccggaa cgctccggaa agttccggcg
    13801 gcttcaggaa ctgtgcgcct ccattgccgc caggcaggaa aaaaccattc tctttaccca
    13861 gttccgctcc atcattcccc atctgcacga tctcctgtcc ggagtcttcg gccgttccgg
    13921 gcttaccctg cacgggggaa cgcctattcc ggaacgccag aacatcgtca ccgccttcca
    13981 gaaggaatcc ggccctccct tctgcatcct gtccctgaaa gccgccggaa ccggcctgac
    14041 gctgacccag gcatcccacg tcatccatgt ggaccgctgg tggaaccccg ccgtagaaaa
    14101 ccaggccacg gaccgggcct accgcatcgg gcagcaccgg aacgtactcg tgcaccgcct
    14161 gatctgccgg ggcaccattg aggaccgcat tgacgccatg ctcaaggaca agcgccgcat
    14221 ggcggacgac ctgttctccg gaggccccga acaatggctc atgaacatga gcgcggaaga
    14281 attaaacaat ctgttttccg gcagtccctg ctgagcaccc tgcgtaagaa cggattcttt
    14341 cctgaactct ccggaagagg gcagcgcccc ttcatccccg gcgttattca tgaaaccacg
    14401 gccagcggca tggtggtttt ccagtctccg cgggtaaaat ccggaaattc cacaggcatc
    14461 ccgccctggg cgatggaacg ggctgtcaac ggcagcaggg aagaccagga agctccttcg
    14521 taaacgttct gatccattgg ttctccgttc cggaggcatt ccacgatgcg ggaaagcatc
    14581 acaaagtcca taccgccgtg accgcccatt ttcgtggcca gctccccgat gcgtttccag
    14641 aggggatgat cgtatttttc ataaatagcg gccagttttt ccctgccttc aatccattca
    14701 tgataattgc cgttgcccag cttttcaccc gccacgcggg ttgggaaacc agccagcgtg
    14761 ccctccgttc cctggatcag gttggcgcgg gagtaaggcc ggggggacgt ctcatccagc
    14821 tggacaagaa tggtccgccc cagctgcgtc ttgacaacag ccgtattcat gtcaccgcag
    14881 atgaatggag tatggttcca gcggtgatcg gcgggaagat gctttttagc gtaggcattg
    14941 cgcccgagag caggagaagc gaacgccgcc acacggcaga aacggtcctc tccacgctcc
    15001 aaattcatat attgagccac cggccccaac ccgtgggtgg ggtacaggtt
//
LOCUS       DAWEMX010000054        15024 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1653_length_15024_cov_9.809272, whole genome shotgun sequence.
ACCESSION   DAWEMX010000054 DAWEMX010000000
VERSION     DAWEMX010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_15024_cov_9.809272"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    433..442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5923..5932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9436..9445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3353 a         4699 c         3904 g         3038 t
ORIGIN      
        1 cgtgatccag ccggacgccg aacaggcggc gcgccgtctc cggctgccct tttccagttg
       61 cgcctggaaa ggaacacagg gccattggga gggaacggga gcaggcaact ccatcgactt
      121 ccagggaagc cgctcctacc agtgggggga tgacccgcgg gatatccatt gggcggcgta
      181 cgccagaacc ggccagctca ccatgaaagt cttccgggcg gaactgtcac cgcaggtaga
      241 cgtggcggta gatgtttccg aatccatgtt ttttcacgaa gagcgcgcgg cacgcacgcg
      301 gggcctgctt caattttgcc tgctctccgc catcggaacg ggagcccagg tgaagattca
      361 tgcggtgaaa gggcgccgta tcatccccct ggaccaggaa gatgttctct ccggccaatg
      421 ggaagcgcag atnnnnnnnn nncttccccc ggatgaatcc atgccctcca tttccatctg
      481 gcgccccaat ggaatgaaaa tcttcatctc cgacctgctc tacccggggg aaccggacaa
      541 ccttctggaa accatggcct ccttaaaagg gatgtccgtc atcctggccc ccacgctgca
      601 ggaagaagcg gaaatccccg ccggcaacgc cagactcaaa aactgtgaat ccggccacct
      661 gcgccgccag ctcatcaccc cttccctgtc ccggaggtac gcccgcgcct acgccgccca
      721 ttttgaactc tggatttctg cctgcctgcg ccgccaggcc gtttttgccc gcatcccctg
      781 caaggggacg ctgacggaag ccctttccgg agaagcatta cggcagggtg ccgtggaact
      841 ctcctgaacc gcacccttta ctctcccccc aagccatgat cccccacttc accaacacga
      901 ccgcgttctg ggcgcttctg gccatccccc tcatcaccgc catccatttc ctccagcaca
      961 aaacaaaaac acaggccacg gccacgctct tcctgttgga agcgctggct ccggaggccc
     1021 atgagggtaa tgcctgggac aggttgcgca tatcacgctc cttctggatg caaattctgg
     1081 ccgtcctcct gcttacctgg gttctggccg ctcctgtctg gcccggcaaa ggctccggcc
     1141 agaccgtagt cttcattctg gatgattccg caaatatggc cccctttcgt caagaagccg
     1201 tgaatgccgt ctccgaagac atggacacca tcctccggtg cggaatcccg gccacatggg
     1261 ttctcatggg cagcagggct tccggacagc ctctctaccg ggggccgtcc gtccgccagg
     1321 ccctgcgcgc cctggatctc tggcagccgc gcgaaggaac acatgacctg gcccctgccc
     1381 tgcgcaccgc cacagccgtt gccggaccaa cgggaatcac gcgcctgatt acatccactg
     1441 cccggagggt ccccgccggc caatccgcac ggggtgtagg gaaacctctg gaaaatgcgg
     1501 ggtttgcggg aataaccccg gtggaggccg ccggccccgg ccattggagg attgccgtta
     1561 aaaacaatag cccatcccct cttcggagcg aaatctccat ccatacggaa ggcgggcgtc
     1621 ctcctgcccg gcgttccctg ttcctgaatc caggaactgt cacggaattt gaatacagcc
     1681 tccctcctga aagcggaaag gccgtgctcc ggctcccgac ggacgccttc ccggcggaca
     1741 atgagctcct gctggtccgc tccgctcccg cccccgttgc cgtcagcatg gaaattccgg
     1801 aacaatccgg aaaaatcttc cgcaatatca tcaatagcct gccgggattc tcccccgtcc
     1861 cggacggctc agatcccgac ctcctcctgc tggagggaaa aacggaaaac gccagtcccc
     1921 ccggaaaagc ggccatcata tttgcagcca gcggcaaacc atcttacggc gctgtcacgg
     1981 cggaacggca tccgctgact gacgggctga actggagcgg cctgctcatc ccctccatcg
     2041 gttccatgaa gccgggggaa aaagccggca tcctgctctg gcagggggaa tcccccctgg
     2101 cctgggtgga cgggaaacgc cttttcctca actggccctg ggaaaaatcc aatgcggacc
     2161 gcgttccggc gcccctgctc atgacccgca gattcatgca atccgtacag gaaaatctgc
     2221 ccggaacaca ttacggcaac cttcccggag gaactctctt atccatgccc gcaggcggaa
     2281 aactcattca aaccatgccc gggggagaac gccgtgaaac tgtctttaac ggcaggctcc
     2341 cggaagaaac cggatatgtg gaaatcttcc cccccggaga agggaaaaca cccctgttcc
     2401 aaggctccgt ctggttctcc gacgcccgga tgggagattt ttcccactgc tccacgtttg
     2461 acacaggact tccccagccc catgaagagg cgctccggca catgaaacgt gaccctctgg
     2521 cctccctctg gctggctctg gcgttcctgg ccctgattct ttcctggctc cctcccgccc
     2581 cagacatctc cctgcgccca tgattcaatt caacaccccg gaatggttct tcctgatccc
     2641 cctgcttctg gcggcaggct ggaaatacag gcgtctgcgc ctggcctccc ccctgcgcct
     2701 ccttctgcag gcctgcctgc tgctggccct ggctcagcct gcccttgaca ggggcggcaa
     2761 aggcatggac ttgtgggtgc tggtggaccg atcaaattcc acggcgggaa ttccggcagc
     2821 cggggcagag gaaatccagg ctattctgga acggcacaaa catgccgggg accgcgtccg
     2881 attggtggat ttcgcgcaat ccgccgtgct ccggggacgc ggagaccccg tctttgccgg
     2941 gggaaccgcc ggaacggaca tggaacaggc cctggcctat accatggccc tgatgtcccc
     3001 gaaccggacg aaccgcatcc tgatgttgac ggacggctgg cccaccacgc cgctggacca
     3061 ggcggcggaa cagctcatcc agtcccggat tccggtggac taccgcctgt ccgtcacctt
     3121 ccgggaagcg gacatccgca tagaacaaat ccgaaccccg gcgcgtatcc ggcccggcga
     3181 agcctttatt ttagaagcaa ccctggcagg ccctcccggc ccggacatca ccgttccgtg
     3241 gcaaatcagc aaaaacggag gggctccgct gcgggggacg gctaccctgc tcaacggcaa
     3301 agccacggtg aggctggcgg acaggatctc cacacccggc tgttccgctt atgaaatgac
     3361 cataacgccg caaaacgatc caatccctga aaacaaccgt gccgtcagct tccttgaagt
     3421 gacgggagga aacggcgtcc tgctgctctc cggctacgac cgcgaccccc tggcgccttt
     3481 cctggaagcg cagggattcc gcgtccaaca accgtccggc ctgaaccggc ttgatgcccg
     3541 ccacctctcc ggcgtggggc tggtcatcat caacaacctc tccgcatcct ccgtcccgcc
     3601 ggactttctg catgccctgg actattacgt gagggaacag ggcggtggcc tgctcatgtg
     3661 cgggggcagg catagtttcg gctccggcgg atacttctcc tcccccattg acgaactgct
     3721 ccccgtttcc atggaaatga agaaggacaa gatgaagctc atgacggcca tgagcatcgt
     3781 tctggaccgt tccggatcca tgtcctgttc cgttcccgga ggaaagacga aaatggatct
     3841 ggccaatgcc ggaacctgcc agaccatttc cctgttgtcc gaccaggatc tcatctccgt
     3901 ccacgccgtg gacagcgaac cgcaccctat tgtcacgcta agcagtctgg gccctaaccg
     3961 gaagaaaatg atatccagcg tctccaggat tgcctccatg ggcggaggaa tcttcatcgg
     4021 cgccgggctg aaagcgggct ggcaggaact gcaacgttcc gtggccggaa cacggcatct
     4081 gctcctcttt gcagatgctg acgattccga agaacctgcc gactaccggg aaactctgaa
     4141 agaaatggtt aaggaagggg ctaccgtaag cgtcatcgca ctgggaacgg aaaaaagcgc
     4201 cgatgccgga ctgctcaggg aaatagcgga attgggacgg gggcgcatct tcttctgcga
     4261 ccgtccgggg gatatcccca gcatctttgc acaggaaacc gtcagtgtgg cccgggccgc
     4321 cttcatcagg gaacgcaccc tcctgcgcgg cacggctggc tggcttcaaa ttgcagccgg
     4381 ccagccggaa tggccccctg ccgtagacgg ttataacctg tgttatttaa ggaatggagc
     4441 aacagcagcc tgcgtaacgg aagacggaaa cgcggcgccc ctggtctcct tctggaacag
     4501 ggggacggga cggacggccg ccgttacgtt cgccatgggg ggagaactcg gaaaaaacat
     4561 ccagcaatgg gatggctacg gagaccttat ccaaaccctg gcccggtggc tcaacaggaa
     4621 aaatccccca cagggatact ccgtgcgggc ggacacggca ggagaccggt tacggattcg
     4681 cctttactac agtgaggaaa acatcccgcg cctggcggaa cgcatgccgg aaatatccct
     4741 ggaactctcc ggaaaagaaa gctcccgtac acaaaacggc atctgggaac acctgcagcc
     4801 gggaatattc cagtgcagct ttcccctccc gcacggagtc atggcgcggg gcgcggtccg
     4861 catcggcggc agcgttatcc ccttcggccc tgtcagccag caggtggacc cggaatgggc
     4921 catgccacct gaatccggca acgctttcct ggacctggtg aaccggaccg gaggcaggga
     4981 aaggatggac cttccctcca ttttccggga accgaggccc ggaacaagcc tccaactccg
     5041 tcccatcctg ctgtggagcg cctgcgtcct ccttgttctg gacgctctgt ttaccaggac
     5101 cggcctgctt ccccggggac aaccgcggca gagggttacg cgctcccggc cttcaggaga
     5161 aaacaatcaa tctatctgaa aatttccttc agggctgact gttcctgagg agtaaggcaa
     5221 tcagaccaca cggcatctcc accgcgggcc ggacgagccc cccggccgcc gtgttccagc
     5281 ccatttcctg ccccttccgg ggagggagcc gccgactcca cccccgcagc ttcccccaga
     5341 gcggcgcggc tcaagtcctc atgaaccgcc tgtctggaaa ccgtgtcaag ccgggggcgc
     5401 tcttccatct caaaggcaag gggagcgtcc ccgcgtccac gccccgttcc tcctcgtccg
     5461 gattcacttc cctctccctg cccggcttcc atcacggtac gctcatccgg agacgccatc
     5521 agggatatgc cgcactgccc gcacatgctc cgcagctgtt gggcggcccg ttccagctgt
     5581 ttccgcagac ggagggccgt ctccggatca atctgccggg aaggcaggga agccttcagc
     5641 tcctggagct ggcggctcat gatgccgccc ggctgcaggg caagggactc cattcccgtc
     5701 aacgcctcct gaagggactg catcgcctcc gggctggacg ctccattcag gctgtccagc
     5761 agggaaaggg cggaatccgt ccgatacatc tgattggata atccggcaac ggccgctgcc
     5821 gttttcccct tcagggcatc cgccgcttcc agtccggcat gggaatacat ctcattccgg
     5881 tgcattcttt tcaaatcctc caactgctcc cggaaaggct ccnnnnnnnn nnctcttatt
     5941 atccacgctc tccatctccg ccaggcggtc caggacttcc cccacccggg ccagaacggg
     6001 cggcaaatcc ggaagctgcc ctacggcttg cggcgcgggc aacggcaaaa aagccccgca
     6061 agccataaga accgcgcctc ccgccagcca ggccaggcac caggcggaac gcatccgcag
     6121 cacggaattc ccctgtaccg cgctttcccc tgcctccacg gcattgcccc attcctcacc
     6181 ggcgctcaac gccgcatgca accccagctg ttcatccaga aaggccgccg tatcacgcct
     6241 gctgaaaaaa cggccccgga gccgcagcca cccccacacc gccaaaaata aacctgcggc
     6301 ggcaagggca cctcccgcca gccagcccca gtcagccccc atcctgcgtc cgccgtacca
     6361 gaccatacca gctaccgccc cggcggcaaa aaaaggcatg ctaagacact ccaccgccag
     6421 cacggcattc agcagccact gcatgcggga aattctcctc cgccattcct gtcccacgat
     6481 taagggcata accatatcat ggctcaacag gttcctcccg gaaagacaaa aatgcttttc
     6541 cgcaaacgaa tgggggaacg gccctttcca tagtttcccg gcattgccgg acggagggaa
     6601 atttcagcac aggcgggcga tatccgccat caattcccgt acgtcttcct ctcgggtggc
     6661 ccagctcgtg cagaagcgca ccgtgctgtg ggtttcatcc tcccggcgca aataggcaaa
     6721 accgtatttc ctccccagct cctccagaac ggaatcgggc atcgtgacaa actgttggtt
     6781 cgttccgctg gaaaagggaa gcgccagccc tctggaacgg caaaaatccc gcagcatcac
     6841 agccaggcgg tccgcatggg cgcccagccg gaaataaagg ccgtcgcgga acagctccag
     6901 gaactgaatg cccagaagcc gccctttcgc aagacggccg cccttctgct tggcgatgta
     6961 acggaaatcc tccttcaggg cctcatgccg cagaaccagg gcttcaccaa acagagcgcc
     7021 taccttcgtg ccgccgatgt aaaaagcatc gcaaagggag gcaatcgccg gcaaatccag
     7081 gtcattgtcc tcatcgcaca agccgtagcc gaggcgtgct ccgtccatat acaaatacag
     7141 cccccgccgg cggcaaactc cggaaatttc ctgaagttcc tttctggaat aaatggtgcc
     7201 caattcggtt ggctgggaaa tataaaccat ccggggctga ggcatatgct cacgggtctc
     7261 gtcatcccag tgggcgtgcc acgcctcctc aatttgccgg gccgtcagct tcccgtccgg
     7321 ggaaggaatg gcggaaacct tgtgtccgca agcctccacg gctcccgact cgtgcacatt
     7381 gatatggccc gtgtccgcgc acagcacgca ttgatggggg cgcagcgccg ccgcaattac
     7441 cgtaaaattg gtctgggtcc cgccgataag gaaatggata tccagatccg ttctgccgca
     7501 caagccggca atcagcttcc gggcctggtc gcaatagggg tcctccccgt aaccggcagt
     7561 ctgctccata ttcgtttccg ccattctgcg gagaatatcc gcatgggcgc cttcactgta
     7621 atcacagttg aaaagaatca ttgccttgct gaaaaacgcg gggaccatat cccccggaac
     7681 ctttgatgta aaggaatatg agaaatacac cggaaccgga acagcgctgc tggaaactcc
     7741 agaaaaaacg gaaggccgca tgcagctgga gaaaatgaat gccatgtacc ttaaaaaggc
     7801 aataataacc ggcttccccg cccatacacg gaaattgtcg tttcccgcag cctaagaaaa
     7861 agccatagtg tcttcatgca agacgcatca gaacttggag aacagaaaga catgtgcctt
     7921 tccatttcgg aagtctcgga attgacaggc atcacgccgt ccaatctgcg tttttttgaa
     7981 gaagaaggaa tcctggacat cccgagaaac ggggcgggat gccgtgtctt ccaccgcaag
     8041 gaaatggaag aacttctggg gctggtttgc cttaagcatt ctggattgga actcaaggag
     8101 atcaaacgct tttttcacct ggttcgccaa ggtaattcga cattgcgcga acgttaccgc
     8161 atgttgcagg aagggcatca ggccatgaaa acgcgcattc gggaactgaa aaaatgcctg
     8221 aaatactcca aaatcaaaat ggagtatatc ggccagtgct gcgaggcata tgacaaaggc
     8281 gaacctctgc cacaggtgaa cccggaatgg atggattcct tcctaaaaca aaaaatttga
     8341 agaaggtgct gacgcctggg cctgctgccg ggaggggcaa atggaggcta ctcgcttcac
     8401 gcaacaacac atccccttct tccggcagaa tgctttcaga aggaaatgtt tttcagacaa
     8461 tccgtaaaaa cggaatcaac aaggaattcc ggatagtatg gaacgcctga cgaacgttct
     8521 gcggaggccg ccgttgcgat tgccgtttat cccggcaaac aaatcgggtc aatgccctac
     8581 ttgcctttgc tgttccgcgt tgtagcgtgc tccggtcacg ggtgtggcgg ctacggcgtt
     8641 ctccagttcc gcccactcct gcggagagca ggtaaaatca agcgtgtgca ggttttcttc
     8701 cagatgggac agtttagtgg tgccgggaat gggtacgata taaggagctt tttgcagtag
     8761 ccatcccagg gcaacctggg aggaagtcat gccgcgcgtc ctcccaaact gttgcagaat
     8821 attgacaata cgcatattgg ctctcatggc ctccggcgtg aaacgcggca gggtctggcg
     8881 gttgtcgttg ttcgggtcaa attgcgtgta ttcattgatg cagcccccca ggaatcccct
     8941 gttgagcggg ctgtaaggga caaagcctat acccagttca cggcaaacat ccagaacgcc
     9001 attgttttca acatcccggt gcatgagatg gtactcactc tgaatggctg tcaggggatg
     9061 gacggcgtgg gccctgcgaa ttgtaccggg agtgacttcg cacatgcccc agcgccgcac
     9121 cttgccttcc ttgaccagtt cggagatggt ttccgctaca tcttcaacgg gaaccctggg
     9181 atcaaagcgg tgctggtaga ataattcaat agcgtccacc ttcaaacggc gcagagactc
     9241 gtcgcagtag cgccggatgg tttccgggcg gctgtcctgg cggcccgtcc cctttccgtt
     9301 aataacttcg tgcccgaact tggtagtgac gctgatcttc cccctgaagg gggaaagagc
     9361 ttccccggcc agaagttcat tgctcagggg gccgtagatg atggccgtat caaaaagagt
     9421 cactccgcgc tccacnnnnn nnnnncgcga gggagactgg ctgcggttgt aggtcattcc
     9481 catgactccg aagccaaggg gagaagcttc caaggcacag gagcccttgc caagcgtccg
     9541 gttccgggag gcggcaaagc cgccgttttc cggagaatcg gcacccccgc cctctgcccg
     9601 ggcaatggcc ggagaaatca gcgcagccgt accgaataag gcggacattc tgagaaagcg
     9661 gcggcggccg tcagaatagt gtttatacat gctcattaaa cgttgatggt tgaaattttg
     9721 ttatataatc cgagcatatg ggttccacca actggaagtc aacaagggaa tacagcaatt
     9781 tatctcagga agcgccttgg aacaaaaagc tggctcggaa aacaaatatg catcagaaac
     9841 gcatctgaca ctccgcctgc aacggaagca aatatccgcc ttcttctgca acagcgccgc
     9901 tcggaacggg acgctttcct ttccccctcg cacaacaaat tgctgagtgc cggtaagcgg
     9961 acaatttaca aaccttgctt ggtgcgttca aaaataatgg aatcttcact tttcccgacg
    10021 gaggattcat catccggccc gactttctcc aactcctccc ggaacacccg gagcatcggt
    10081 ccgtggtaaa agcccagctc ccggctgatt tttttccgca gatcctctcc ttcataactc
    10141 cggccgtcat gccgtaacag tttcaaacgt tgaagctgga ggaggatcag gctttctttc
    10201 acggcatcgg aaatctcggg attgtaaaaa agaacttcca ggatgccctg caggcggtcg
    10261 cttttccttc gggcccaggc atcttttcct tcgcgtcgca gggccccaat ataccgtgta
    10321 tgcagccgga ccatgctgga aataatctgc ggatcattcg gccaacgccc gagaagcctt
    10381 tcggaaagat tcaccgtatc ggcaagttcc ctctggttat gttcccggaa attcctggca
    10441 taccggagtt tcttcagcat cagctctacc agcgccaggc cgtattccgg ccgttccgga
    10501 tgggcgtcgg caagctggag gagcagcacg gctgcattcg gttcaatgcc gggaatgcgc
    10561 ctcttccaga aaaggcgggg atttccggcc aaaagctgtg cgtattgaaa acggtattca
    10621 ggatggtcag gatcatcctc caacaatgtt tccaacattc cgaacgcccg ggaacgctca
    10681 acactctccg gagaaccgga caaagccagc aacgcgcgga ccgtttcaaa ccgatcctcc
    10741 gcccgctccg acatcgcaaa gcggtcggcc acctgccgga aaagttccgt tgcctcttta
    10801 tctttaccct gttttttcaa agccgtcgcc aactggttga cgggaaaagc atctttcctg
    10861 aatttcatca tgttgcggaa cgcttcttcc gcaagagcat aactcccggt gcgcagtgcg
    10921 cattccccga tgacggcatt ggcctcgcaa acctttgact ccggcagatt ttttcctctg
    10981 gcgatcatcc tgtaataggg cagcaaagct gaaagcagct gcgtattttt ctgtgaaggg
    11041 ggctgctccg caatacgggt gaacacttgt gacaaagccg catcggcaac tgcggcattg
    11101 ttttccgcca gtttcagcgc agacgccgtc tgcagataac cggcgcccaa cgcgacaacg
    11161 aaagcggccg cacacagggc cgccgccaaa gacaataagg caacagccgg tttacgtttc
    11221 atccacagct gcaaccgcct ggccggagac gggaaggagg ccccgaccgg ttcatgattc
    11281 aaaaaatgca gcaaatcttc agaaagttcg tccatgcttc gataacgcga ctccgggcgg
    11341 aaactgacgc acttgttaat aatcgcggcc aagtcgggtt cgctgcactt caacggcgga
    11401 accggttccc tgcaaatccg ttccatcaat tcttcaggag ctatccccgg aaacggcggg
    11461 gattttgcaa caagctcata aagcgtcacc ccaaacgcat actgatcgct ggaaaaggta
    11521 ttgactccat gcaccagccg ctccggggac atataccgca gcgttccgct ctgagctcct
    11581 tccttctcaa taacttcatt tctcccctga agcagaaaag caagtccaaa atcgctgacg
    11641 tgtacttccc ccgcttcatc cagcaacagg ttcgccggtt tgatatcgcg gtgcagaatg
    11701 ccgcagcggt gggcgtacgc aatcgccctg gcggcctgga ggccaatacg ggctatctca
    11761 tacggattac tgaattcaca tccgtccaga ctcttgcctt ctatcaactc catcgcataa
    11821 taacaacatc ccgaacggca cgcggcactg aaaattctga caatattggg atgatgaagc
    11881 ctggcaatca catgcgcctc atgctcaaac tggatgcgtt gggcggcatc ctgcagcagc
    11941 aacggagaca acagcttgac ggccactttc cggttgaggg acaattgcag cgcttcaaat
    12001 accaccccca taccgccgct gcctagtctc cgaatcagcc ggtaatcgtc cccctccaaa
    12061 tcgggaaaat cgtgaaccgg aggcataatt ccctgttttt tttcaacgct ccaatcttcc
    12121 atctccatca caagcggaag aagtgccagc aactcggaag caaactccgg atgtgccccg
    12181 gcaaaatcct gaatatcggg aacattgccg gaacggcact gatccaggaa ttgttcaacc
    12241 agatcttcca gagaaatctt ccccttcatg gctgaaactc ggttaactcc atcagctgct
    12301 tcctaagccg ctgcagcgcc cgcacatagc ggatgcttgc ggctttcggc acaatatgca
    12361 ggacttcggc acaatccgca ttggacatgt cgtcaaaatg gcggagttca agaatctgcc
    12421 gatcgttttc gggcagcgcc tcgaccgctt tccggagcaa tgtataacga tccgccctga
    12481 cggcacgggt aagaggcccg gtcacggtat ctgcaaaccg gttccaatcc aaccgggcag
    12541 cggacccctc gccgtcatcc gtcacattca cttccctgca ggcgtctctc ttgaggcatt
    12601 gcagatgctt cctttccata gccgtaaggg tctggaacag aatcgtccgc agtttgaaat
    12661 agacaggaat ctccggattg ttttcaaaaa aagccatttt cccgcaggct gccagcagag
    12721 cctcctgaac cacatcttcc gccgaaacgc gccgggcaag aaccggattc aaattcctcc
    12781 gggccagggc aagaagcctt ccccggtatt caaggaaatg gcttccccaa gcatccgggc
    12841 atgctgtttc tgcttgcgtc attgtaccgt gtctttttta tcctttctca ttttccgggc
    12901 agatagacat atcccgccgc actccttcct atccggccga ttccgggata cggcaggtgc
    12961 attccggcga ccggcttcga ggaagaagcc gcaaactcat aaaagcgccg ccgggcctga
    13021 accgctccgg acggattcat atcgtaaatc gtgcaaacct ccggacgcgg aatctggagc
    13081 gcggcagcat gaagcaaatc tccgacaatc agcagggaat cagtctcaaa aacggtatgt
    13141 ccgggtgtat gcccggaagc gtcaagagcc ttgatgccgg gcagaacttc ttctccaaac
    13201 cggaatgttc tcacccggct gccgtaagca tgcaacacct tccggacctg ttttccctgt
    13261 ggtccggccg taccattttg ccaaaattcc cattccggca tggaaacata gacgagggct
    13321 ctggaaaaaa cggcttcgcc atgtttccca agcaatcccc cgatgtggtc gccgtgcata
    13381 tgagtgagca aaatgaaatc gacgctctcc ggaaaaacgc cgctctgttc aagtttacgg
    13441 agcatcccgc cccgttttcc tccgttccct gcgtccacca gaatgatttt tccgtcgccg
    13501 cgtttcaaca ggaaaacatt cacggaagaa ggaaccctcc cgcctccggt taagcggagg
    13561 aactcctctt ccggcatacc cgggaataat tcgaaccgca tcgctgaaag agcatcctga
    13621 acagcaatca cctcaaaatt tccaaactgg aatgaacgat tttccgtctc agccgcgatc
    13681 acgatgccgg aaatcatggc ggctccacac aacaaccatt ttaccataaa acgcctttct
    13741 tctcaatatg ttgacttttt aagacatgat gggcaatgta tcaaagctgt acggaagtca
    13801 gatatgtttc cgcactacag gtgataaaac gtccttattg atggaaaaac gctgccgggc
    13861 tgcaggcccg gcagcagata agatgacggc gactattttt taccgccgtt tatcctgcct
    13921 tttttcagac cgtgtttcaa acctttcttg aggccgtgtt tcaggccgta cttcaacgcc
    13981 ttcggtttct ttttcagtat ttgaacagaa aaggaggctg ttgttgattc cgccgcgttg
    14041 gcagtaaccg taccgggagc cagaccgaat attccaataa ttgcaggaat aatgagcttg
    14101 ttcatgataa tcaatgaggg ttgatatttt ttctgaaacg gtaccgtttc acttttcaag
    14161 aatgcagttc cttagggaaa tctaccaatt cggccggaat ttccatcagg tctgccagcc
    14221 agattctatt gcctgtaaga aactgaaacg catggaatcc cccctcaacc ttcgcggacg
    14281 acccggaaac cgacatcact gaaagcgaaa gaagattccc ttccttctcc tttccgctca
    14341 gttcggcagc tcgtccgcgg cgaacgccag gaacctccct tcaccgccat gaccgtttta
    14401 ccgtgtgcca cggccttgga aacttcaact gaagaagaag tccattccca gcaattgccc
    14461 cacatatcaa tcgctccgca ggcgccggat gtcgcggcat gggcgtccac gggagatgtc
    14521 ccgtttctct cgccgcaatt aaaatccgca tccttgggca tatgcccggc ggcaaactcc
    14581 cactcctctt cagtcggcag gcggtaaacg gctttcctat cctggagcga caaccattgg
    14641 caataggcag aagcgtcgtc ataggaaaca ttcaccacgg gatgcctccc tttgccggca
    14701 ggcatggcgc cattatccca acccttagga gccttccttc ccgttgcctt caggaattgg
    14761 gcataatcct cattggtaac cggagtatat gcaattgata cattccagga agctccgagc
    14821 accggcaaat acccgccatc tgcattgcgg gcacctggtc tgaatcgcgg atcactgaaa
    14881 cgccgcatgc tttggtcgat gcgcgcttcc ctgttttgaa ccatttcatc cacgatctcc
    14941 tgcatttcct gtatcttgga cgcatgccgg gccgttcgtt tcaattcttc aagcttcgcc
    15001 ttttttcggt caaggacctt gtcg
//
LOCUS       DAWEMX010000055        14924 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1665_length_14924_cov_10.172708, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000055 DAWEMX010000000
VERSION     DAWEMX010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_14924_cov_10.172708"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5272..5281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5601..5610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3588 a         4493 c         3638 g         3175 t
ORIGIN      
        1 caacgaacaa ccatgaagaa cacattattt ctttccggtc ttatggcttt gtgttgcggc
       61 gcagggatgg ccgccaccgt agcaatcgac ctaaatgggg aggcagccga aggggcatct
      121 ctgcctgcaa accttaaatt gtctggattc ggagacgtca acagcggaag tgtcatcact
      181 gcacaaaaag tcgtcacatg gttgcaaggc accaccggct ggcaaggnnn nnnnnnnaag
      241 gcaccactgc aaacggcaat tccggctccg gtatttacgg cagcgtcacc gcatccgggg
      301 atactgatgt atccgtgtct ttcatcaaca gaaatgctta cggcggctct atcgtaggaa
      361 cagcggtaac gcttaccagt gattacgcca gttataccgg attgactctt tccgtggact
      421 gcgccgggct gcaatccgga gcaaacttca cgacgctcaa tatcgtttat cagacggcag
      481 aaggctggca gatcagccag ttgaccggcc aggcctcttc cctgattcaa acaggcatga
      541 ccctgtccgt ggacttgagc ggtactcccc ttaccagcga cacggcttac attgtgatgt
      601 ccacccgcaa tggcggcgga aacacgaact ccaacctgct tttttccatg tcagccactg
      661 ctgaggatgt cgttccggaa ccggctacgg catccctgag ccttttgggc ctgtccctgc
      721 tgatgctgcg ccgcaagcgc tgaaataaaa aacgcggtct ttcccgtttt tcaacaactg
      781 tccggggtgt agaatcccgg acagtttttc atttttaagc ctgaaggctt gcaatcgtat
      841 tgccatatga ccgggctgcc gtataatggg ggcggacgga aaatatccgt cattttctga
      901 caacaatcat gaaccaagca ccccatgttg ccatcgtcgg cgccaccgga gcggtgggcg
      961 ttgaaatcct gtcctgcctg gaaacacgca attttcccgt aggttccctg aagcttctgg
     1021 cctccgcccg gtccgccgga aaacaggtcg ctttccgtgg gaaaatgctg actgtggaag
     1081 aattgacgga aaaatccttt gatggggtgg atatcgccct gttcagcgca ggcggaggaa
     1141 tttccctgaa attcgctcct atcgcagcgg ctgcgggctg tgtggtcatt gacaattcct
     1201 ctgccttccg gcaggagccg gacgtgcccc tggtggtgcc ggaaatcaat ccggaagcgg
     1261 ccttcaacca cccccgcaat attatcgcca acccgaactg caccaccatc attacgctga
     1321 tggccctctt ccctctgcat cagcgtttcg ggttgaaaac cgtcatcgcc tccagttatc
     1381 aggcggtttc cggcagcggc cagcacggca ttgcggaact ggaaacccag gtgcgcgccg
     1441 tagtggacgg ccaccccgta gtgaaaaatg tttatcccca ccagatagcc ttcaatcttc
     1501 ttccccagat cgattccttt acggaaaacg gctataccaa ggaagagctt aagatgctta
     1561 atgaaggccg taaaattctt tccctgcccg aactcaaggt aacatgcacc tgcgtgcgcg
     1621 tgcccgttta ccgttcccat tccatctccg ttaccgccca gtttgaaaaa cctgtggact
     1681 tggaaactgc ccgcagcgcc tatgaaggca aacccggagt cgccctgatg gacaacccag
     1741 cggagggcgt gtggcccacc ccgctggaca gcaccaacgg agatacctgc tacgtagggc
     1801 gcatgcgcat ggacatggcg attgacaatg ccctgactct ctgggtcgtg ggcgatcagg
     1861 tccgaaaagg ggccgcactc aacgccgtac aaatcgcgga attgctggtc aaccgctgac
     1921 ctgtttcctc cacaccccgc acctattcca taacgccatg tgtgaacaat cactgaacga
     1981 ctacatcaca gaacaatgcg ccctgattga cgcagcgctg gacagactgc tgccagcaga
     2041 ggacgaaagc cccgaaacca tccacaaagc catgcgctac agcatatttg cgggtgggaa
     2101 aagaatgcgc cccgtccttt gcctggcggc ggcggaggcc tgcggaggac tggcagtgga
     2161 cgccctggtt cccgcctgcg ccgtggaaat gatgcatacg tattccctca tccatgacga
     2221 cctgccagcc atggacaacg acgatctgcg ccgtgggaaa cccaccagcc acaaggcttt
     2281 cggagaaggc gtcgccatcc tggcgggaga cgcgctgctg acggaggctt ttgccgtaat
     2341 cgcccaggtg gacgatacgg aacgctatac cgtcagggat tacgtgaggg agctggcagc
     2401 caccggcggc agcaccatgc tcatcggagg ccagattctg gatttggagg gagaacacaa
     2461 gcagctctcc gaaccggaag tccgcaccgt gtacgaaggg aagacggccg ccctgctaac
     2521 cacggccctt cgcttcggcg gcatgtccgc aaacgccaca gagtcccagc tggaagctct
     2581 gacggacttc ggttacaacc tggggctggc tttccaggtg attgacgaca ttctggacct
     2641 gacggcttcc acggaaaaac tcggcaagac cgccggcaag gacgtcaatt cccagaaatc
     2701 cacctgtccg gccctgattg gcctggacgg cgcccgttcc gaagccaaat gccgcactca
     2761 ggcggctctg gatgccctga tgatcttccc ggaagaacgc cgcgcccgcc tggtggaact
     2821 ggccaactat cttcttaacc gcgaatatta agccgctttt tctttcatgc ccgcagaaac
     2881 cgtatccgac aacgtggaaa ccctgatcaa tctggcgctg gccgaagact tcggctccgg
     2941 cgacgtgact tccacatact tcgtcccgga acatctgacc gccagagcta ttttgacccc
     3001 ccggaaaaaa ggcgttcttt ccggcgtcaa tgtcgcggcg gaagttttcc gcaaggtgga
     3061 tcccaccctg aaggtggaag tttacctgca tgacggggaa gccgtggcgc ccggcgccgt
     3121 ggtgatgctt attgaagggt ccgcccgctc cattcttggg gcggaacgca ccgccctcaa
     3181 cttcatccag cgtctttccg gagtagctac gctcaccaga caatatgtga aagccatttc
     3241 ccacaccagc gcccgcattc tggatacccg caaaaccact cccggctacc gtctgctgga
     3301 aaaagcggcc gtgctccatg gcggaggcac caaccaccgc atgggccttt atgaccgcgc
     3361 catggtgaaa gacaaccacc tgatgacgga tggaaatacg gaacaccttc aggaatgcat
     3421 caacaggctg cgtcaggaaa agcccggcgt ggaaatccag ctggaagcgg acaccctgga
     3481 acaggtgggc aacttcctta aactggaagg cgtggaccat atccttctgg acaacatgac
     3541 gccggaaatg ctgaaacaag ccgtcgccat gcgcggagga tgcaccaccc ccctgctgga
     3601 agccagcgga ggcgttcacc tggacacggt agccgccatt gcggaatccg gcgtggactt
     3661 cgtctccgta ggctacgtga cccactcggc cccctccctg gacctggggc tggatttcag
     3721 cgtagaataa ccactcatcg ttcaacacgc catggacttg tccaatttca gagaagaata
     3781 cctcaaaggc cagctgcaca gaaaggacct ggcggaaaat ccctttgaac aattccaaac
     3841 ctggtttgaa caggccctga aagcggatat tccggaaccc aacgcttttt ccctggccac
     3901 ggcggacgcc cagggcaggc cctccctgcg caccgtgctg ctcaaatatt ttgaccggac
     3961 gggctttgtt ttcttcacca actacggcag ccacaaggcc cgcgatattg aagaaaatcc
     4021 gcaggtctgc atgatgcttc cgtgggtaat gctggaacgc cagatcatca tttacggaaa
     4081 agccgtcaaa gtctcccgcg cggaatccct gaaatatttc ctcacccgcc ccaaggaatc
     4141 ccagcttgga gcttgggttt cacaccaaag ctccgtcatt tccggaagaa aactgttgga
     4201 aatgaagttg atggaactga aaaacaaatt ctccaaagga gaaatacccc ttccctcctt
     4261 ctggggcggc taccgaatta tcccggatac ctttgaattc tggcagggcg gccccggccg
     4321 cgtgcatgac cgtttcatgt acaaacttca ggaaaacggc tcctggacaa ttgaacgcct
     4381 tcagccatga ccgtccggcg cacaacaaac caggggcttt ctttcctgga cgtaacaaca
     4441 ccgctctgca cggcatccct gtgccttcag ggagcgcacc tcacctcatg gaagccggca
     4501 gggcatgagg aatgcctttt tctttctcct aacgcagcct ttgtccccgg caaagccatc
     4561 cgcggaggca tccccgtctg ctggccctgg ttcggccccc gggaaggagc accgtcccac
     4621 ggcatagcgc gcacctcgga atggcgcctc caccaccagg aaatggacaa gtgcggcaac
     4681 gtcctgctct ccctggcttt ctatccggaa gatgaatcct atcccgccgc catcctgcgc
     4741 cttaccctgg gccgcacgct ggccatgagg ctggaaacca cggcgcgcac gcagccctgc
     4801 aagctcacgg aagcattcca taactacttt gccgtcggca acctgaccaa atgccgcgtt
     4861 catgggctgg aggacatccc gttccgggaa gaagccgccc agcccatgaa gcacggagaa
     4921 cgcccgctga cgccgctggg gtgcttggac cgcgtttaca acttcccctc ctgttcggga
     4981 aaaacaatgc tggaagacct catgctgaac cgcgccatca cggtggagcg gaaccatgcc
     5041 tcttccgtca tcgtctggaa tcctggacaa cagggcgcca aaaacatggc ggacctgggt
     5101 gcggagtcct ggaataaatt cctgtgcatt gaaacgggaa acgcggagcc ccgttccatt
     5161 tccctcgctc ccggacaaac ccataccctt taccaaaaaa tttccgtaag gcatatggag
     5221 gaagcctgct ctccccgtta aaacggcaga cagcattacc tctcaagtaa tnnnnnnnnn
     5281 nccccggcaa caggaaaaac cccgcggaag accatcaatg acgcccttcc gtggataaac
     5341 agaaggtcct tacagcttaa tttcagtatc ccccttcctt actgaataag tattgccatt
     5401 aatgattaaa gagtaagaag cattcgacgt gaccgacacc gtgcctttac catcaaaaac
     5461 aatatcggtt acaatatcgc caaacctcaa attcctgatt ccatggcggc ccttgttttt
     5521 tttcaacctc caccggactt cctttccggc ggcagacact ttcttaaatc ccagcacatt
     5581 ttcaataaac aacgcaatcg nnnnnnnnnn attccggtct tacacggcgt ccgtgttcag
     5641 tggagggggc atctccgctt gggctatagc actcccagac agtatgcggt tccatattcc
     5701 tgtaagcggc aagctgctgg ttgataacct tctcggcaat agcatcggcc tcctcatggt
     5761 agccatactt ttcaatggcc ttaatcgcca tatatgtcgt cggcagccag attcctcccc
     5821 gccagtaatc cccggtttga tgatgataat ccttatccga gcgggccagg gaaggagtcg
     5881 gataactgcc tccgaattcc ccgggattca tcagatgatt taccatgctc cgcgcctgtt
     5941 ccctggacgc aatccgggcc agaaggggcc aataggaagc aatggtttta acgcggcagg
     6001 gctgtttgtc cgcaatggtg acatcgtaat aaaatccatc ccgctcatcc cagtaaagat
     6061 ggttgatctt tttcttaagc gcttcatatt cagcgttcca ttttcttgct tcattttcat
     6121 ttcccaaagc ctgttccatg gtagcaatgc agtgggcgct aagagcctgc tgggaaatgg
     6181 catccaccca tagcacctta tggtatcctc cggcatcacg cccgcgggga gtattgtcca
     6241 taccgctcgc tctccccgtc caggtaaagc catcatcccc aatggaattc aagtaaatac
     6301 gctgggggga acattcaaac cgttcaccag ccttagcccg ggcaaaccat ttgaaatgct
     6361 tctgcaaata ccgcttttca ttaagaagat gattaagccg cctcttatct cctgtgaaat
     6421 caaaatattc cttttccacc caagcaaaca gcgggggatt atccactaaa tggattctaa
     6481 ggggcgtagc ggctttttca tggataggtt tgtaaagatt atccaggctt tctattccgg
     6541 gaaacgccct gggcgcatac ttggcaaaaa gcaccatgaa acaggtgtcc catatccaga
     6601 tttgatcttc gtagcaattt tcatccatat agggagacgc gggaagccct tccggccctt
     6661 tcctgacgcg cccagccgca atttcccatg tttggtgata aagttccgtc atttcccggt
     6721 ttggatatac tggcagggga atctcttttt tccaatcccg gttcatgcag gaatccggat
     6781 gagttgttgc ggaaaatccc tgcagcgggg acgacagcag gagaatcgaa cctgtgattg
     6841 ccgatagtct ggcgttcatt tattccgtac tgattaaatt aaaaaactac ttcttcagaa
     6901 gttccttgag cgcagcatca gccataatca cctgcccatc ggcattggga tgaacgccat
     6961 cgggaaaaag ttccggttta tcttgaaaag gggtgaaaaa gtcgatggtt ttacaattat
     7021 tgtccttcgc aacctgcttc acaagaggga caattttatt tttcaccgtt tccttgtcaa
     7081 tttggctgga agactgccac gagggaagcg gaattgcgca atagatttga ataccaggca
     7141 tgttttcccg cagttcggcg atgatggcgg cataattctg cttaaactcg gacaggaatt
     7201 tcctgttctg cggtttggcg tcattggtgc ctaaagcgat aatggcaatc tgtgcatggg
     7261 aattgagcgc attccggtac tcgcccgttt taatataggg aaggtctccc ttggccagaa
     7321 gggtacgccc gttaacgccg cagttcagca cttctatttc tttccgtttg aattttttcc
     7381 tcaattcctg accaagaaga tggctccagc gcaaaacatc cttattggct ccggctactc
     7441 ctgctccaaa agtattactg tctcccaggc acgcaacgcg caaggggacg ggctgtccgg
     7501 taacggaaag aacaaaatct acgatatggg caggattgtc aaaaccgtgc ggatgatgcc
     7561 ctccggaccg gcggatgact cttacaggtc cccccatccc ggcgtaacgc ttagcgaaaa
     7621 catccgtatt ctcttcaata gggactacgt catcttccgt ccgggaaata aacaacaaag
     7681 gaattccggc tttagccatg ccgcgcatat tgtccacggg gttaccctta taggacaaag
     7741 cctgctgttc attaaagccg taggcgcgca ggcatttgtt ccagtcatcc ggcgaacctt
     7801 tgcccttgcc cctgcccgcc ggccagcttt tgaaatcaca gacgggggca tccacataaa
     7861 cgccagccac gcacaaagga ttcttctccg cccagttaag cgcataaagg cctccacggc
     7921 tcagtccaaa caacaccgtt tttctggaaa aaccgcgttc atccaccaga tacctgtaaa
     7981 attgatccat cacatccatg gccttgggag aaccgtaaag gtcttccatg ctgataaata
     8041 ccgccgcata gccgcgcttt accagttcaa gatctgctat aggcttataa tcaaaaaatt
     8101 gcggccgcca tatccacaac tgttcttttc cagaaacaat agccttgtcg ggaaagacga
     8161 tgaatgccct gcggccgccc acggcaaact cctcccgtaa aaaaccattc cagacagaaa
     8221 ccgtcctagg gaactgacgc ttggccattt ccggatcata ccccggaatt tccggccgtg
     8281 catgtcctgt ttttaccagc cattcattac ggactttctc atgttttttc atcgtatccg
     8341 gcgtcggccg atggtctttt tccgcaacct caggagcaag gcctatttcc gggaccagcc
     8401 cggcaagaac ttcccgggct atcatatggt gcccttccgg accggggtgc acgccgtcac
     8461 cggaatacct gaaaccggga ttcccggcct tctctctggc aatgagtttc cgaagcccgg
     8521 gacggatatc tatgactttc cagccgtctt ttcttttaga aaccagccat tccgcgtact
     8581 tgtccaatac agcgtcgtac tcagcctgtt cagcggaatc cccccggaac aatggaggag
     8641 taatgaagac aatctccgct tttgccgcat cggcagcctt tttaaggcgg atattccctt
     8701 cctggtacgc ttgaaaacgc gctctgtcaa acgcctgcat gaggccgtca ttcatcccgt
     8761 agcaggccag aatcaattga ggctggtaca tcttgagaat ccggctgagg cgctcatgca
     8821 ggcatggacg ggggaatctg ccgcccgcat ggccatattc gctcaacccg gctaccgtct
     8881 cgctgggaac ggcaaaattg acaatctcgc aggctgcaaa ttttttatcg tccctcaagg
     8941 tgttttccac ttcattggcc caatggcccg cgtaagcaat actatcaccc agaatggcaa
     9001 tccggccctt attcaaaggc tgggcatgaa gccgcatccc cgctcccgca caacacagca
     9061 tcatcaacaa taaacatctt gctattaaca gaaaacgcat ttcacaataa tatggttgta
     9121 cttattacta ctgaaaacaa aataaatttc ttgcaagata cgggaaaata ccgatcggca
     9181 ggcacccgcg ctgtttcatg aaacaaaaaa cccggcactt caccaactcc cgtccggccc
     9241 tcttcccgac aatcacggtc catcccttcc tttaaagcgt ttccctcctt ccccaatttc
     9301 gccattgccg ccggagaacc atagaaaccg gaaaaccgtt tacgcttctt cccgaaaacg
     9361 gatgggggcg gaagaagtcc ttttcctgaa gaaatagggc aggattcggc ttgccaaaaa
     9421 gtctctttta aggtttggta tgttccgagc cttttggcgc gttccctatc aagatgcata
     9481 caaacccacc ttccgatgtt ggcaacgccg caacctttat tgcggaaatt tacggtcagg
     9541 atgtcttcaa catggagacc atgcgcaatt acctgccccg ccccgtttac aaaaaactgc
     9601 tggctaccat ccgcaaggac gaaaaactgg accccgacat cgccaatgaa gtggctcagg
     9661 ccatgatgac gtgggctctg gaaaagggag ccagccacta cacgcactgg ttccagcctc
     9721 tcaatgggag cacggcggaa aagcacgact cctttgtgga cgtggacccg gagggaaatc
     9781 tggaactcaa attttccggc aagagcctgg tgcagggcga gccggacgcc tccagcttcc
     9841 cttccggcgg tctccgcgcc acctttgaag cacgcggcta caccgcatgg gaccccacca
     9901 gccccgcctt catcaaacgg acggaaaacg gcgccacgct ttgcatccca accgctttct
     9961 gctcttacaa gggacaggcg ctggacaaaa agacgcctct cctgagatcc atgactgccg
    10021 tcacccggca ggccaagcgg ctgttgtcct gctttggcgg tccggacaac attcatgtca
    10081 gcgccgacct gggggcggaa caggaatact tcctggtgga caagcagctt tatgccctgc
    10141 gcccggacct gatgatgtgc ggacgcaccc tcttcggcaa tgtgcccccc aagcaccagc
    10201 agatggaaga ccactacttc ggctccatca aggaccgcgt gctggaattc atggtggacg
    10261 tggacgaagc cctctggaaa ctcggcatcc cggccaaaac gcgccacaat gaagtcgctc
    10321 ccggccagtt tgagatcgcg cccatttttg aaagccagaa cctggccgtg gaccataata
    10381 tgctggtcat ggaggtgctc cggaaaaccg ccaacaagca tgacatggtc tgcctgctgc
    10441 atgaaaagcc cttctccggc atgaacggct ccggcaagca caacaactgg tccctctccg
    10501 cccccggtta cggcagcctg ctcaatcccg gttccagccc gcaggaaaac gccatcttcc
    10561 tcactctgct ctgcgccacc atcaaggccg tggatgaaca tgcggatctg ctgcgcgcct
    10621 ctgtcgccaa atccggcaat gaacaccgcc tgggtgccca tgaagccccc ccggccatca
    10681 tttccatctt cctgggagat ctgctggatg aaatcattga gcaaatcgag aaaggaggca
    10741 ctaaaaaagc gcgcacccaa aagaccatca acatcggcgt ggacaccctg cccatgttcc
    10801 ctctggacgc ctctgaccgg aaccgcacca gcccctttgc cttcacaggc aataaattcg
    10861 aattccgcgc cgtaggttcc tcccaaacct gtgcatggcc catgacggtt ctaaatacca
    10921 ttgtggcgga aagcctggat gaaatctgca ccattctgga acctgtcaag gacaagcccg
    10981 aggagttcca cgccaccctc aacaagctcc ttcaaaacat catcaagaag cataagcgca
    11041 ttctattcag cggagacggc tacggagaag cctgggtaga ggaagcggaa cgccgcaatc
    11101 tccccaacat ccccggcacc attgaagccc tggccgctct ggaaacgccc aaggccaagg
    11161 cgctttttga aaagtacaaa gtggtcagcc ccgtagaact ccacgcccgc catgaaatcc
    11221 agacggaaat attccacaag gaaatcaaca ttgaagcgga aacggcccta ctgatggtgg
    11281 aaacccttta catccccgcc gtaacggaac agctcacgca gcttaccacc gccattgccc
    11341 agatgaaggc atccggaatc aaggcgggaa tgaaagctac tacggatcgc gccaaccaaa
    11401 tcggcacgct tctggatatt ctccccggac aggtgcggga actgcgccgc gccgtggatg
    11461 aagccgatac cagggccatc cagacgggca tggacaattt gagaagcacc attgatatgc
    11521 tagaaagcct gacggacgca gacctgtggc ccgttcccac atacgcggaa ctgctctttc
    11581 tgtaaagcag gcgggctttt ctggaataga aaaacactgt ctatcaatca taaagactac
    11641 ttatcccagt gctttccgca agaaaaacgg caaattaatc gcctgacgga ttgacaaaac
    11701 aatcaaaata gttatccttt tcgtccggtt gtgcataggc cgcttagacg gacggcactg
    11761 aatctagtaa ttcacaagaa gcatctttca acatcatgag caagaggact tatcaaccat
    11821 ccaagcgttg ccgcaagcgt cagttcggat tccgtgcacg catggccacc aagaacggag
    11881 cggacatcat ccgccgccgc cgcgctaaag gccgcaagcg cctgcttccc aagggagccg
    11941 aaatccagta caagcgccac accatccagc acggccgtta atccagccgc caggatccgg
    12001 ttaacgcctc cttggaactt cttttcacgg gccgacccgc gggagatgcg tcttactcgt
    12061 caacaaagca tgaccagggc attccaattt gcccgggtac gcaacgaggg gccatccgta
    12121 gcaggtcggc ttattgtttt aagcgcagcc ccgttggaac atccggagga accctccaaa
    12181 ttcggtatca tctgtacgaa aaaaatcggc tgtgccgttg tccgcaacaa actgcggaga
    12241 cgcgtgaggg aaatactgag ggcgcacggg gctccttttg aaaacggcgt acacctggtg
    12301 tgcgtcctcc gctggagggc cgtggaagcc agctacgccg acctggaacg ggactggcgg
    12361 aaagccgccc gaaaacttaa attgcaactt ctttcccggg aacaagacaa gacatgatga
    12421 aatggctgct catcaccctt gtgaaggggt accaaacctt catcagcgct cccctgcatg
    12481 tcctgggggg gcccgggagc ggctgccgct atacacccac ttgttcccag tacttcatcc
    12541 aggccgttca catccacgga gcctggcgcg gctttcttct tggcacatgg cgtattttaa
    12601 gatgcaaccc gtggggcgga tcaggttatg atcccgtgcc cccggcgcgc cacccacgct
    12661 gaacaattac ctttcttccc ctttcctttc accaactcca atcaagctat atcacattat
    12721 ggatcgcaca gcatggatca tcataggcat ctgcgccgcc cttctgggcc tgaatattta
    12781 catgggcaac aataaaaagg ctgaaaccgc ccccattccc gcaccgcagg cggctgccgc
    12841 cgcagccaat gccccggttg ccggcgtccg ccccggagcc ggaacctccg ccccggaaca
    12901 ggcctccgtc aacaaagccc ttgaagaaga caaaaccgcc ccgatcactc tggtagccac
    12961 gcaggaagtg aacgggaggc aggaaccctt catcacttac acgttcaacc gcataggggg
    13021 ttccatcggc acggttaccc tgcacaacga tatcgtggac tcccaaaaag tcgcggacca
    13081 taatatcacg attaacgaag cccagcagcg gggcatcgga gaactcgttt tcaacatgga
    13141 cgccacccag gatccttcct atgacaacac cgtgtataag gaagtgaaac gcacggcgga
    13201 ctccgtaact ctggaaggct atgaccccgc acggcagctt ttcatctcaa aaacgtacac
    13261 cctgcacccc gtcaaaaatc tggaagggga agtattgccc ggcagcaaat atctcattcg
    13321 cctgaccgtc tccctgctca ataaatctcc caatgtccag gatctgcgct acatgggcat
    13381 cttcggcggg agtgcctatc ccatcgccaa aagcgagcca aaagatacct acacccattt
    13441 cttttaccat gcagacggct ccctggaaca ggaagtgcct tcctatttca caggcggctt
    13501 tttcagcagc gccaaggccc gcgtcctgga aggccctctc caggacctga cttatgccgg
    13561 cgtcatgagc cagtattacg ccaccatcct tcttccccag gaccagtcca gggggtccac
    13621 cgtgtacgcc acccgccagg aatttccttt gacgcatgaa ggcaacaccc tggtgcccgg
    13681 cgtcaccgtg gccatgggaa tccccaatct gaccatggcc cccaatgaaa tcaagacgct
    13741 cacctacgat atttacaccg gccccaaatt caacgcctac ctgcgtgacc tgaatctttc
    13801 ctatcccgcc atcagcgata tcatggcata cggctggctc gtattcctga gcgttccgat
    13861 gaactggctg ctcaacctct tccacggatg gttcggcaac tggggcgtag ccattatctg
    13921 catgaccatc gttgtccgtg ctctcatctg gccgctgcac aaaaaatcct atatggccat
    13981 gaaacgcatg tccctggttc agccggagat ggtcaaactc aaggagaaat atccggacga
    14041 ccctcagaag gtgaacatgg aaatgatgaa gctgtaccag aaatacggca tcaacccggc
    14101 cagcggctgc gtccccatgc ttatccagat tcccattttc tttgcctttt accgtgtgct
    14161 ccagtattcc gcagaactga gggggcagcc cttctgcctg tggatgacgg acctttccct
    14221 gcctgatacc gtgggccacc tgttcggcgt tccaatcaat attctgcctc tcatcatggc
    14281 cgtaaccatg attatccaga tgaggatgac gcctcaggcg ggggaacgct cccagcgcat
    14341 tattatgaac ctgatgcctg tgatgttctt cctgttctgt tacaatttcg cttccgccct
    14401 ggccctgtac tggaccacgc agaatttgat ctccatagga caaacggccc ttatccgccg
    14461 cttgcccatg cccgtgcttt cccccgccaa aaagaaaaag tcgggctttt tccaaaagat
    14521 gatggagcag cagcgtgcgg ccctggaaga acagcagcgc aaagctaaag gccgcaacat
    14581 gcgcaatata acgcctaaaa agtaggaatc cctgctctgc cttccgtctc cgcaagtgcg
    14641 tccgcccccg cggccgcact ttttcatgga aaggcgcaga aaaaaatatt ctgccggcgc
    14701 aagcctcaaa aagggaaagg aactccctta cagaatttcc tcaaaaaacc ggccctctcc
    14761 acttggagag ggccggtttt cctcgtcata tattcattgc gtagttaatt ggagcagctt
    14821 ggcagaaaat gccaaacacc cactggagca aacctaacgg attatgaatt cgcggccatt
    14881 ccctgaatac cgaaattaca atagttccaa tcaggtcggg tgca
//
LOCUS       DAWEMX010000056        14910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1666_length_14910_cov_10.182228, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000056 DAWEMX010000000
VERSION     DAWEMX010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14910
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_14910_cov_10.182228"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2922..2931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8063..8072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11705..11714
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14433..14442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3045 a         3751 c         4451 g         3623 t
ORIGIN      
        1 ggaacacgtg gaattaacgc gggcgcactg ggtgaccctg cgtgatgtgc tgtggcgtaa
       61 ataccagcgc aagcggtgcc cgtggcgttt cattgaaacc atagacaagc ttctggaaga
      121 gatgccggaa gaggaggacg ccgattcccc cgatgggaag ggcggacgat aggagcctct
      181 ttcaactttc tgccgtgcgg ggaatgctga ggtcggttgc cagggaatgg ctttcctggg
      241 tgtatccttt tgtctgcgag ttatgcggcc gcggcggcct ggatggctgc catgtttgcc
      301 cggactgccg cagaagtttc gtgcctgtcg agcctccctt ctgtgccgtc tgcggggaac
      361 cggcggaggg ttccttcatc ccgtccggat tatgccgccg ctgcgcggcg gctctcccct
      421 cttttgagga agcccgtgcc gtgtatgtga atatgggatc attgcgggat cttctgctgg
      481 ctttcaaata tgggggagcc gttcatctgg ccggctcctt cgcgcagatg atggcagagg
      541 cagtgcttgg aaatcctcac tggttcggcg ggaaaaaacg cctgatcgtt cccgtgccca
      601 tgcaccgcgg caaacaggct caaagggggt acaaccaggc gcaggaactg gcggttctgc
      661 tgggagagga actgggctgg ccgtatgccg gcgtgctgaa acgcctgccg gatacgcttc
      721 cccaggccag cttgtcgcgg gagcagcggc tcagacatgc ccgtaaaatt tatgcggcgg
      781 atgaaaaaag gatgaaacgc agccccgtca ggggcaggga tgtgttgctg gtggatgatg
      841 tttttacgac cggagccacg gcagactcct gcgcccgtct gctcctccgc tccggggctg
      901 cttccgtctg cgtgctgacg ctggcgcgga cgcatcatgc atggcgcggc tgaaccggaa
      961 ttttctttca gcccgcctgt ttccttttta caatggcagg catgaatccg gagctgatgt
     1021 ttcatacgga agatgaagtc atgttttatg atacggactg cggcggggtg gttcataacc
     1081 tggcctattt gcgcatgatt gaggcctgcc gtaccaaact tggcgctaaa ttgggaatgg
     1141 attacaaaac catgagcgat ttgcagcagt ttgccgtggt ggtgcgccat gagattgact
     1201 atgtgcgtcc ggctgttctg ggagacacca ttttgacgac gggatggctt caatccgtgg
     1261 accgggcccg cttctggtgc gattttgaaa tgcggcgcgc ttccaataat gatctcctgg
     1321 tcagggctca tcagcagctg gccctggtcc agatgccgca ggggcgccct gcgcgtattc
     1381 ctgccgaatg gcgtgcgaga tggctggaat gcatggaatc ctgatctttt tttgttcatc
     1441 ttccatttta cgcttgccat gaaagggggt gtggtgttta ctgcttgcgc ccttccagca
     1501 agggtaagtg cttgggtagc tcagttggta gagcagtaga cttttaatct attggtcccg
     1561 ggttcgagtc ccggtccaag taccattcct tttttgaaac cctgtggact caatagttta
     1621 cagggttttc tctttcttgt ttcagatgca gaaaatggcc caagtgctat ctgtttcact
     1681 tacaggttaa tagcattata tggcggtaac cgttctggtt aaaaagatgg aagtagttga
     1741 ttgtaaatag gccggaatat atatatccca ggaaattttt aagggatatg gcatcgggcg
     1801 tagcaggcta ttctgtccgt actgccgtca tgggaatcca tcccggacgg cgcgttctat
     1861 ttcatgaaac aacaggaaat ggtcctttaa atggtacagc catgatttaa cgtcggccaa
     1921 ttcatgggct tccagcatgg tgacgggcga aatgaacgga ttctttttaa cggcgatttc
     1981 gataagcccg atgatttcat ttctcttgct caggacggga atggccagat actgcatgta
     2041 tttttcctcc ggaggctggt tgcgcttccg gaaaaagctg cgctgcactt cttccgggct
     2101 ggaaagaacc agggggctga ggtggcgttc cgcgaataat tgttcatcca gcatggcggg
     2161 tgttttcgtt acgggccgcg gaggctgtgc cagcagggaa ggggtgtcgt tattgtgttc
     2221 tccctgtgcg tagcctgtaa cgtgcagcaa ggtttggtct ttccgccgct ctgcctgaat
     2281 ataaagcact tcaaagacaa gggagttttt atattcagcc agcgcagcgg caatggtgga
     2341 gcacaggaac aggcaggctc cgttgaagct ttccgtcgtc ccccttgaac cgtccgtttt
     2401 tgcctcccgg caactttctc cattcagttt attggcgaag ttttcgtatt ctccgctcag
     2461 ggcggcgtaa gtttttttgt aaagagccgc actgcggttt ttcagggcgc aggcatgctc
     2521 cctggtttcc cggttcctgt tgagcagtct ctggcattcc acatgccata caatgaaagc
     2581 ggcgcccaga atgatcaaaa ggactatttt ccccgtattg gctccttcca tgggagttcc
     2641 cccgatgatg gagatcacga caaccagaat aggctggaac agatttgctt ttttgaggaa
     2701 agagggctct ttgaacataa aaagacgctg tgcaagtttt gttaaaggtg tgttatgaat
     2761 tgattatcaa aaatattcca tatcaggtgt gacggctgtg cagaacagag gtaaagtgca
     2821 gaaaggtttt ttcttatggc attttaaatt aagtttattt acaatcaatg agttttttgc
     2881 taatttagaa gtattctaat acggtatttc tttgaatgat annnnnnnnn nataaggatt
     2941 cttttattcc aataattgtt aggaaaaaag aagagttttc cttttgggaa atgatttggt
     3001 aaacagcggt gttttgccta gttttaacct tataaaggaa ttatttttga ttacagggca
     3061 aaaaataggg cataggaaaa atccgaatga agaagcggat ttttatgtaa ttccccttca
     3121 tgggaaagga tggcaaggag tgtgaaaata atgaatggtt cccggaatgc ccgggaggca
     3181 gaaatgttgc cgcggacggg aataactgtt attctctgct ttttcttttc cggtgaatca
     3241 catgccgttt cccgttactt gaggaaacgg aaatcagaat tttcttccgg aaggggattc
     3301 ccatagaaac gtttagggcg cgtatgcccg ttttcctgcc gtgctgtgtc attcggcatg
     3361 gttttccgcc gcccatttcc tgtactgcgc ggacaattgt tctacaagcg tctggtgcct
     3421 ggatgccaga ttgttctgtt ccgtcctgtc cctgatgtcg tacacctgcc aggggccgcg
     3481 gttgggggcc accagcttga attgacttgt cagggccgcc cggtttctct gatgttccca
     3541 gaagatagtg cggggagggc aggtattggg aatgaagtcc cacaccttgg acggaggaag
     3601 ttccggagga ttattccagt aggtgttgag cggcttgccg tccatatggg ccggaattgg
     3661 gagagcggcc cacgcaaggc aggttggggc gatgtcctga agcaggaaag gcgagtggta
     3721 gcgcgatccc ggacgtattt tggaagggta gcgaatgatg aagggggcgg acagccctcc
     3781 gtcaaaggtt tctattttat actggcggta aggggtattg gacacagccg cccacatggg
     3841 gccgtatccg tccctgcatc cgacttctcc cagcgggcct gtggggaggg tagtcttctt
     3901 gtttttattg ttctggggca tttccgctgt tgcgccattg tctgataaaa acatgacgat
     3961 ggtattgtca ctgaggcggt gacgttccag ggacgcgaga atgcggccta tgccgcggtc
     4021 catgataacg atttgggcgg cgtaggtggc catgcattcg gcaatataat ccttccgctt
     4081 tgcggaagtt ttttcatagc cttctggatt gaatttgccg gccatgccca gtttggcggc
     4141 aggagggacg attcccagca gcttttgttt ttccagtctt ttggaagcaa tggcatgggg
     4201 ggatttgccg ttgtagaacg agagcatttt ttctatttcc ctccgggggg cctggagggg
     4261 tgtatgcgga gccgtataag ccacgtaaag gaagaagggc tgcctgcttt tgacggcatc
     4321 gtcaatatag gccagagccg ttttggtgat atcgtccgtc atgtagaaat cctccggcag
     4381 atcctccaga cggagcagtt tcccgttttc accgaagggt ctgtccggca tgggcctgta
     4441 gtaatctgcc gctccaccca gcaaaccgcg ggaacggtcg aagccgcggt cccatggata
     4501 ggatccccga acttttccaa gatgccattt cccggaaaga taggtacggt atcccgcttt
     4561 tttcagaagt tccggcaggg tggggatacc cgggttcagg cgtccgcggt agccggggcg
     4621 cccgctgtcg tcatccagga gccctacgtc caccttgtga ggttcgcagc cggtcatgat
     4681 ggaggcccgg gagggtgaac agcgggaagc ggtgtagagc cgggttgcca gcatcccctg
     4741 tctggccagg gtatccaggg ctggagtggg tatttccgaa ccgtagcagc ccgggtcgga
     4801 ccatcccatg tcatcagcca ggataaggac gatattaggc ctgtccgcca gtgaagggac
     4861 aagggtaagc aaccagaagg ctatgctgga catgatggag gaacgcaggg tagagcacat
     4921 gaaaggaata tggaatatca tatataataa gcggaaccgc tattgattct tgcagcagga
     4981 ggaaaaaaca ggccgttccg gaaagatccg tcctgcgtat gcctgttttt gccggaggcc
     5041 ttctcctgcg gctgaatgtc ttttctgcgt gatgaagcag tgccggaaga ggcggcctcc
     5101 ctaccaccat aggatggcgc aaagcagaat ggcggctcca taggccattc ctcccaggca
     5161 aagaggcgtc caccaagcag gtttggagca tattttattg accatgtcgc ggtacaggta
     5221 gggcttgcct atccagagaa tcgaaaggaa aatgacgatg taacaccaga tgggaataag
     5281 gagcctggtc actggctcct gaaggaaggc ggcactgagc aggggagctg ccaccatcag
     5341 gccgaagatg cctagcgccc gtggaaagag caggtttttc acctgcgtga ccatcagcac
     5401 cagaaagacg gggcatgcca gctggagcag ccagcggata ccttcaaatt ccgccagccc
     5461 cacccgaaag cggctcagca cgcccaatcc ttcgggagct accagcagaa agaaccagaa
     5521 caggccgccg gccagcagaa tctgagccag caggtggttg cgcggagacg ccagcagaaa
     5581 agcctgcacc tgctgccttt ttaccagcgc atacaggtgg accagcaaca gaacggttcc
     5641 caggatatac cccgccacag acagtggtat cattccatcg tacggatgca ggaagggagc
     5701 atgctggttc atctggacgc taaccttaat gattatcccg gtttcgtaaa ggcgaatcag
     5761 tgggagtagg aagaaaattc cactggattg ccggcaatgg tccggcagct tcccgcccgc
     5821 gccgggcgga gaaacaaggg gttcaggaac cttgggtgac tttgttaagc gtagggtcct
     5881 caacggggga tattttcccg tccgcataaa gcaccatgat tcctccctgg tggcgttcaa
     5941 gggccgggac tacgatttcc tcaaaatcct gttccgtgat gacgctgcgc ccgtcaatcg
     6001 ggatgccgtc gctgatccag ggtttgcccc gcagggcctg gccgcgcgcc acattcatag
     6061 gacgcccctt ccggttgtcc agccagctgg tgtaggaaat ggtgtccggc ccggcgggaa
     6121 ggttggcatc ctccgctccg aggatttccg gacgcttgtc gtttttgcag cggaacgtgt
     6181 ccgggagcat gttgggttcc ttggcgttcc gggcgatcag gtcgggttgt tccgcattaa
     6241 cgatgggcgc cagggcgtcc caccagttcc ggcattccct ggcgctcagg aggctgtctt
     6301 tcatgccgct ggagggcagt tttccgccgt tctggcctgc gaattccttg ccccagtcgc
     6361 taatgcggcg caggttgtcc gtacagcgcg ttctggctgc cgtttccgtg gcatgggaat
     6421 aaatgctgta ggagacgcct gccagcaggg caatgatggc gatgactacg agaacttcaa
     6481 tgagtgtgaa acctttgctg cgtggggtaa gtaagcgggt tttcatgatt tcccgccatt
     6541 atgcacgatt taggctgctt gaaaaggcag aaactgcgct cttgggtgaa aagagcgagg
     6601 ccggtgctcc ggagatgaaa aaggccggtc ccgtgaagga ccggcctgta gaaggggtgg
     6661 cgccggattc cgcttattta gcggaagtta ccagagcagc gaaggattcc ggcttcaggg
     6721 aagcgccgcc aaccagagcc ccgtcaatgt ccggctgggc catgagttcc tccacgtttt
     6781 ccggttttac ggagccgccg tactggatgc gcaccttggc ggcggcgtct gctccgaaga
     6841 cgtcggaaat aaccttgcgg ataaaggaat gggcttcctg ggcttccttg gaagttgcgg
     6901 tcaggccggt gccaatggcc cagacgggtt cataggcgat gaccaggttg gatacctcct
     6961 ccggggtgag atccttgaga ccgcccttga gctggatttc cagcacgggt tccaaaatgc
     7021 cccccaggcg ttcgtccttc gtttctccga tgcagaaaat gggggtgatg cctgcggcga
     7081 tggccttctt gattttggag ttgatgatgg catccgtttc tccgaaatat tggcggcgtt
     7141 cgctgtggcc gagcaccaca tacttgaggt tcagttcatt cagcatgctg gtggagattt
     7201 caccggtata ggctccgttg tcgtactggg agacgttctg ggctccggcg gtgatgcagg
     7261 agcaaccgcc cagagcgttc tgcacggagg gaatggtggt gaagggaggg atgatgacca
     7321 cgtcacacgc gacgtctttc cccttaatga ggtttttgaa gctttcgatg aactgggcgc
     7381 cttcggcggg gccgatgttc atcttccagt tggcggcaat gatgggtttg cgggacataa
     7441 tggaatgata ttaatcagga acacgcattg ttgacaaatt gaagctgagg tcaagcacag
     7501 atcaaaaggg ccgcatacgc gccgtccgat ttttcctcgt ggggaaggat aagtttttct
     7561 tctttcagcg tccattccgg atggttctgc agaaagtccc tgaccagcag tccgtcttcc
     7621 tccgcgtcaa tggaacaggt ggaataaacc agtctgccgc ccggtttgac ggcgcgggag
     7681 gcattttcca ggatggtctt ctggagtgcg gccaggcgac ggatttcctc cggagtcagg
     7741 cgccagcgca cgtccacgcg gcgttggatg actccggtgt tggaacaggg aacgtccaga
     7801 agcacggcgt caaagcggcc cttccattcc gtgcggcagg gaagggacca gtccgcctgc
     7861 gccgtcctga cgaaggaaga accctgcctg tccaggtttt ccttcagggt gggcagccgg
     7921 tgttcgtgga gatccgtggc ggtcaggtgg gctttgcctc cggtagcggc gatgatggcg
     7981 gcggatttgc cgccgggggc ggcgcaggcg tccagaattt cctctccggg ctgtggggcc
     8041 agcaaatcaa tggaataacg ggnnnnnnnn nngcctgttt taatttcctc cagcggcaga
     8101 aggccttcca cggagaacca gccgggcgcg cggtccagcg gggcgaggga ggccggaata
     8161 tttttcatgg gcatcagagg attggcgcgc acatacaggc gcggcgtggt attgttccag
     8221 cggagcaggt cgcgggtcat ttgcggcccc atttgttccg tccatctccg taccagccac
     8281 gcgggggtgg aataatgaat ggaaagcgga agtttttccc gttcctccag aatggttttc
     8341 tcccgccgca aagcgttgcg caggatagcg tttaccagtc ctctcaggcg tacggacgcg
     8401 agattcacgg tttcatacac agcggcgtgg tccgccatgc ccagcagaaa aagctggctc
     8461 aaccccagtt ggagaataag acgcgcttcc acgtccagcc tgcctttccg gagggttccg
     8521 atgacgtggt ccagccaggt ctggttgcgc aatacgctga agaccaaagc ctgcaccagg
     8581 tgcctgtctg ccggtggaag cgcggttcgt gaacattccc ggtccacgag ggtttccgcg
     8641 aaggagcggc ctgcatgcca gctcctcagg caattcagtg ctgtctggcg gggggagggg
     8701 ggattgttct tcaccgggga actctacatg cggaacgttc aggtttcaac ctgcaaaaac
     8761 gcccggcgga aaaaggggga tgccgggcca gctccggatt tcacctgtct tttctggggc
     8821 tgcccacttc cttcctgtaa atggcccgga gtttttccag ctggtctttc cttgtttccg
     8881 caagattatg tctttcttca gggtccttgc ctatttgaaa gaggaacggt ttattttgtt
     8941 tttgcgtaag cttccagccg tcccccgtga tgacggtgta atccacgacc acataatcgc
     9001 gctgcctggc ggagttgccg gacagtatat ctatatagga aacggcatct ttaccttccg
     9061 gcatgcggac ccccgcaaga tcggcgaaag aggccatatg gtcgtaattg gccaccaggc
     9121 tgtggcagac cttcccatag ggcagtgtgc cgggccagga aatgatgagg ggaacgcgga
     9181 tgcctccttc atggctggtc catttcagtc cggccagatt ggccatggcg ttgtttggcc
     9241 caacgcggcc gcggctgccg cggaaaacat ccagcagttc cccggtgccg tccaagacgc
     9301 ctccgtggca gccgggcccg cggccgcggc ctttatccgt acggtagtaa agttcatgcc
     9361 cgttgtcgga actgaaaatg ataatggtgc gcttgtccag gcccagcttg cgcacctggt
     9421 ccacgatggc tccgacctgc ctgtccagct tgtccaccat ggaagcgtat tcttctgccg
     9481 ctccggcgca ttccctgtta gttccgctgg cattgccata ggactgacgg atggaggggg
     9541 agcccgcgta cgtgttttct tccggaggaa tatctaccgg gccgtgggga aggttggtgg
     9601 aaaaaaagat gaacatgggc ttgttccggt tttcctccat gaaacgaagg gtttcctcca
     9661 gcattacgtc cggagcgtag gtgactttac cttccctgtt gcctcttcgt ttttctgtgg
     9721 ctcccggact gtagtcttcc ggggttttgc ctccgtcggc gtgggtgttg cctttcaggg
     9781 ggaggcgttc gccgtctttc cagaggaagg agggataaaa gccatgggcc ctctgatggt
     9841 ccatgtaccc tacatagcgg tcccagccgt gccttttcaa ttctccgtgc caggtggtga
     9901 atccccattc cagcttgccg aactgtcccg tgacgtatcc ggcctttttc agcaattccg
     9961 ggaggaacac ttctccctca gcggctttga tgcgtgctgc ccgtgccgct ttctcttcca
    10021 gttgctcatt ggaccagcct ttccgttccg ccgtgatgac cagggctccc gccgtctggg
    10081 tgtaggaatg ggagtggctg tcgtgcacgc ccataagcag ggaagcgcgc gccgggcagc
    10141 aatattggct gctgtagcac cgggtgaaca tgatgccctg gctggccagg cggtcgatat
    10201 tgggtgtttt cacgatttcc tggccatagc agcccagcat gcccatgccc aggtcatccg
    10261 cgtaaataaa aatgatattg ggtttgaagg ccgcggcgga tgccaaaaac ggagagagcg
    10321 aaaatgcagc cgccagaaat gcgtgtgaaa gaaatcccat aacggtcact acgcaggaaa
    10381 tgaaaaggtt attgcaaaga aaaaaccgtc cggaatacct tttccggacg gtttgataaa
    10441 gagtggtacc gttacgggcg cttaattgcc gtagggtttg ttgacgcggc tgaattgctt
    10501 caggcgcaat ccgttgaggt ggatgaaccc ttccgcgtcg ttctggttgt acagttcctc
    10561 gtggccgcct tccatcgtgg cgatttcttc ggaatacagg gactgcgggg aacggcgtcc
    10621 ggcgtacatg acgttgccct tgtaaagctt caggcgtact tccccgttga cggtttcctg
    10681 ggatttggtt accagggcct ggatggcctc gcgttccggt gcaaaccaga agccgttgta
    10741 aaccagcgta gcgtattccg ggatgaggga gtcgcgcacc ttttgcactt cccggtcaat
    10801 tgtaattgtt tcaatatcgc ggtgggccgc cagcaggatg gtgccgccgg gagtttcata
    10861 aatgccgcgg ctcttcatgc ccacaaagcg gttttctacg atatccacgc ggccgacgcc
    10921 atgcttgccg cccagggcgt tcagcacgta catgacgccc agggggctga gcagggtata
    10981 cccgtcgcgt tctcctttcg gggtgacgcc cagttctttc agcagaacgt cgagaccatc
    11041 gtactggatg ccgatgacat tgcccttttc atacaggagc tggatgtatt cggcctggtc
    11101 cggagcgtct tccggggaaa cggaaagctt gtacatttca cggtcggcgg gggtggtgcc
    11161 gtcgtaccag gtatcttcca gcatcccggc ttcaaaggaa atgtgaagca ggttgcggtc
    11221 catggaataa ggctttttca gagactgccg gatcgggatg ccgtgggatt ccgcgtaggc
    11281 gatcatttcc gtgcggcccg ggaactgctg gcggaattcg ggatcgcgcc acggggcgat
    11341 gaccttgatg ttcggggcca gggcattgac ggccagttca aagcgtacct ggtcgttccc
    11401 cttgccggtg gcgccgtggg cgatggcgtc cgcgccttcc cggatggcca gatccaccat
    11461 gtctttggaa atgcaggggc gggcgatgga ggttcccagc aggtagcggc cttcgtaaag
    11521 ggcattggcc tgcatcatcg ggaagatgta gttggtggcg aagtcttcct tgaggtcgcc
    11581 gatgaagcac ttggaggcgc ccgtcgccag ggctttggct tccagtccgt ccagttcttc
    11641 cgcctggcct acgtctgcgc agtaggcgat gatttcggca ttatattttt ccttgagcca
    11701 cttcnnnnnn nnnnacgatt ttcatgtacg tcaatgatta gtgtgcggcg gcatgatgcc
    11761 ccattccccc cccggaggaa aggacaaaat catgtttcgc cgcgggcttt ttcccggagc
    11821 cgccggagac tgctttgatg aaacggttcc aagcggagat gaaccggcct taccggggaa
    11881 agcaacgctc atttcttcca cagctttttc cggaatttac tcgggatttt taccggaacc
    11941 cattggaaaa cgacactccg tacaaaatgc tgaaaatgtc ggccctttcc ggtttccgtt
    12001 catgaatccg gcaatcttcg gccatcatcc ggaacaggag tgaaacagcc gctttcctat
    12061 ataaagatag ccatatcttg cgttcttttt gcatactctc acggaaattc ccagctgaag
    12121 cctgtccggt gaacggttcc tccgccagtc tgcgtaaatg gtgcagttcc cggtcctgtc
    12181 ctccctgatg atgaatttga ttgcgcaatg gatctgaaac aggcggtgcg tgtaggcaga
    12241 actccccatg tcttttttct tcaatattta ctgcagggac ggagataagg gccgtttttt
    12301 acgacggagc ttccctgccc gatcgccgtt ttcatgccgg aatatttcta gaaaaatttt
    12361 cagaaaatgt cctccgagcc ctgggagcgg ggtattttgc atgaacttct ttcaaatatg
    12421 tttcctgggg tgttggagat aatggttagg aagagttaaa taaattagct tcttccatcc
    12481 tgttgcgaat gttccggatg cctcctcacg cgatatgttt gtgaaagaaa caggtattca
    12541 attccatgcg caaagaccgc cggaaaggcc ggatgacatt gaaaacggct tgaatttgat
    12601 aaaatcgaaa aaaagcgctt gatttttcat ccccgtccct ctacttattc cgccgaactt
    12661 atggctaaga agagctggat cgcaagagac aagaaaaagg ctgaaaccgt caagcgctat
    12721 gcagaactgc gtgcgcaatt gaaggctgag aaggattata tcggtttaac catgctgccc
    12781 cgcaatgcaa gcccgacccg tacggtcaac cgctgccttg tttccggccg ccgccgtgca
    12841 tttatccgcc gtttcaagct ttcccgtatt tccttccgtg aactggccaa tgccggcatg
    12901 attcccggtg tgaccaagtc cagctggtaa ggacaggaaa aggttgcagc cttataaaga
    12961 ttttgacgct aggctgattt ttatttagcc tagcgttttg ctatgcccat atacgagtat
    13021 atttctgaaa accctgacga ccccggacag tcctgtccgg tgtgccgccg cggttttgag
    13081 ttgaggcgtc ccgtggaccg tgctccgctt gaaaaatgcc tggtgtgcaa gcatcccgtg
    13141 cgtaaggtca tcagccgcat taatgtgccg gaggtgacca agcctctttc cgtttcagac
    13201 gccaaggctg ccggattcac cgtgctggag cgccgggaca agggcgttta tgaaaaactg
    13261 tgatgacaaa gtgccgggca atatctctgc cttcttctcc gaatatatta tttgaatcag
    13321 aatgaatgat ccggatcctc tgagtattct tgcggccttc ggggccgctg aagcgcccgg
    13381 tgcaggcccg gcgctgggtg ggatgatgct ggcagttgcc ggctttatcc tttttttgtt
    13441 gttgaatgcg ttttttgcgg caggcgagtt cgccttgatg aaggttcgtg aaagccagct
    13501 gcacgccggg gaaggtgttc cggcccgaac ccggaaaaaa ctggcccggg cgcggaaggc
    13561 tgccaagcat cctgatcttt atttggccgc ctgccaggcg ggcattacgc tttcttccct
    13621 ggcgttggga ttcctgggaa cgttttttgt atcggaactg acagctccct ttctggtctc
    13681 cctgggattg ggaggcatgg tttccgttta cggaatcgct ctggccgtta catttatttt
    13741 ctttgcctgc tgccaggtgg tatttgggga gtttatcccc aaggctatgg cgatgcgcca
    13801 gccggacaag gccgccctgg cgacggttcc cctgctgtat ttcttttata cggtgttcag
    13861 atatacgggt attcttggcc tgacgggcgg aatggcgcgg tttgtgctga aatacctgct
    13921 gggcatagac ccccgttcca cggcgtgcac ggtgcacagc acggatgaat tgatgtatct
    13981 ggtggaagaa agcgaacgtt cccgcgagct gacgaagcag gaggctgaaa tttccaaaaa
    14041 tgcccttgaa ctgaacgata tgtgcgtcaa ggacgtaatg accccgcgct ctgaagtgga
    14101 tgtgatggat ttgacggctc cctttgagga aaactgggag cttgcccgga aatcccgcca
    14161 cacccggttt ccgctggtgg agggagacca cctggatgaa gtgaaaggct gggtgcatgt
    14221 caaagatctg ctcaaactgg taggacggga aaatccggat ctgaggagcg tgcggcgtga
    14281 attgcgcgtg gtgccggata cgatgcccct ggacagcctt ctcacgttct ttctgaaaga
    14341 acatgcccac tttgccctgg tagtggatga attcggtgat tctatcggcc tggtattcct
    14401 ggatgatgtg ctggaacaga ttgtggggga tgnnnnnnnn nntgacattc aggacgagtt
    14461 tgaccaggag gaaatgcggg agtttgtgaa aaccggcaag gatacatatg ccgtcaatgg
    14521 ggctattacc ctgtttgacc tggcagatta cctgcctgaa atggatttgg attgtccggg
    14581 cgttactacg ctgggcggtt acgtaatcag ccggatgggt tatattccgg aagaagggga
    14641 ggaattgcgg attggccgct accggggtgt ggtgacgggg tctgacggca ggagaatcac
    14701 gcagattctg ctggcccgcc ttccggagga acaggaggag gaatagtgcc atgaaggaaa
    14761 aaccgcatcc ggatatgagt tttgagatgg aggcccgggc ctccggtttt ctttgcgtgg
    14821 ctggagtgga tgaggccggg cgcgggccgt tggccggtcc cgtggtagcc ggcgccgtta
    14881 ttttgcctgt tctggcggag gaattaaccg
//
LOCUS       DAWEMX010000057        14724 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1686_length_14724_cov_11.942532, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000057 DAWEMX010000000
VERSION     DAWEMX010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14724
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_14724_cov_11.942532"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4744..4810
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3212 a         4152 c         3852 g         3441 t
ORIGIN      
        1 gtccggaaca acgggaaaca ggctgtatca ttgccccgct ccggaacatc tttattcgct
       61 gccgggccgg gagcggaaga acatcccgcc ggcgcacccc ggcaatctaa cccatgccgc
      121 catttaaaaa gactgcacat taaacaagaa aaaccgggcg ccctgcaaca ttcaatggca
      181 tggcgtgagg catttcactc attttttcaa ttcttcggct attctgccga ggttcttttc
      241 cgttgccttt ctcagtttct cctgctgttt ccagtaccat gccgttcttt ccgacacccc
      301 ggcctgattc gccagccaac gcacggcgcc gctcgtgtca cagcctgttt tcttctgaat
      361 gacttccagc agttgagcga catatttatt catggattcc gggttgatat tatttcgatt
      421 cccatgcccg gctgaattcc ccgtcccgga acagggaggc aatcccgctg agctgcttga
      481 ggcgcaacaa ttcctccgcg tccatcccca gatttttgag tatccacgca tccgacatgc
      541 ccgcctccgt caactcctgg ataatattgg tcatgagggc gatattatga gatccccggg
      601 cacggttgtg cctgatggtg gaagccatgc ggtttccaag aggcttgtcg attacggcca
      661 ccggcatcat gccaccctcc cgttcgtaaa tatcgcggta agtcttcatg atctggcagc
      721 ggtggaaccc gtccacgatt tcataggtcc cgtcctccag cttataacaa acgataggca
      781 tcgtataacc gtcctccttg atgctctggt agagcagctt catttcgggg ggagctacgc
      841 tgttcgggtt ataggcattg gaacgtatct ggtccagggg aacgcgccgg attttgtaaa
      901 cgggggattg ataggaggtt ttcataaaat attgcggtat ttgttgacgg ctgcccggcg
      961 ccgggcaatc gctcccttgg ttggaccgaa cccggcgtat ttgcaaaagt aatcattctt
     1021 catgatgcag atgcacatgc gcttgtagga aggcacctgc cggaaattgc tgaccggcat
     1081 gtcgtcggga tagcccataa actgaatgac gtcgcggtcg acgtaacggc ccgactttcc
     1141 cgtcacggta gccagaggat aggcggccag cacttcatcg gcagttccgg gatcgacggt
     1201 gccccctttc atccagtact ttttagacct ctccaaaatg ttgttgtagt gttccgccgt
     1261 tttctcgttc attgtggaaa gcaggaattc ataataggac ttccaggtat ggccgggcgg
     1321 cagtttgatg gttttccagc ccatggctgt cgtacctccg tacaaacccg tgaagttgac
     1381 gccgttgacg cgcccgacca tgcgcgccca gttggcagga tcaataactt tgtaaagctt
     1441 caggctttcc tgggcgcagt cattaaacgg gcttgccacc cgcatttgcc cgatcgtaag
     1501 gcctgcctgg tacaggagat cgtaaaggcg gttatagtca aaaccaaaac gggcgttggc
     1561 cgtccagacg tcactgacgc gccagtcata aagagggtag gcgctgacgg tcacggcatc
     1621 agcccgtgta atccacttcc ttccctcata agacgtcctt ttattgccgc gggcaacggc
     1681 ggcgtatctg ttgaggcttt catccgcccg cagccctacc atgacggcag tacttccgtg
     1741 ttttttggca aaatggcgtg aaagcttatc ctggaattca tagtccacca taccgtgccg
     1801 gaacgggacg tccagattct tttcgttgat tacataggga ttttccggca tgggtcgcac
     1861 ccacagtttt ttctgggcgg cgctccacgg cgtccagtaa ggttccccca gggaacatgc
     1921 ggactgggcc ctgatcggca cacagtacca catcttacga atgccgggga attgctccag
     1981 aaatgttctg gtgacatact cggtcgtcat ttgatattgg gcctcataat caaggtggta
     2041 catggacagc ttgtccagaa ggccctgttc ggcggcgtaa cgatagcaaa gattaagcat
     2101 gacgccgctg tccttgccgc ccgaaaaaga caccaggacg ttgtcaaact cttggaaaca
     2161 ataggctatg cgttctaaag ccgcctcata aacattttta tccagatatg ttttcacaac
     2221 ttttccaaag cctgtctttc gccatccgcc gccagccccc tgccggacgg gaacacgtcg
     2281 gcccggatat gattgccgga agccatgcat tttccctgaa accactcttg cagcttgcag
     2341 ctcataggga tatcccctgt tctttcaaac agggttgcaa cgccttcatt tctgcataaa
     2401 taagcaacat catgtttttt catgttttca aatgcgcagc agcttatcgt ccgttatttt
     2461 ccgtcaacaa taccaaccta ctgaattatt gcggcgggtc aatgttttta ctgcaattat
     2521 ttcagtaatt ttattgtcat caccctcctt ctcaaaatac ctgcgctttt tagcgagcaa
     2581 caccattcca gaaatgcctt atgcattgcg ccatgacctc ctttccttct ccgcaacatc
     2641 cgcgctatgt gcagcattca agacgctgcg ttggaatgca cggttccaaa atcggagata
     2701 caaaaatcat aacttgctta aaattaggtg aacgaaaaaa tgtacgactc agagccattt
     2761 tccggttcca acatctttcc cccaatactc tttccggaac cgcatcttcc aggcagacga
     2821 tacatccatc atttccctcc tgaaggaaaa ataaccgtgt ttcccttttt gcaatccggg
     2881 atgctgtcca aacatgaata tgccggtaca cgccttaatc atgccgtcag attagacttt
     2941 aggcgtttta tgattttccc cgaaacgcca atttcatgcc agaacggaat caaaaaatga
     3001 catttagtca gcacttatgg ttgtttttcc gcggtttttc acctttccag cctcaaaaca
     3061 gacaaaaaag aagccctgta atccattaaa attacagggc ttagaaaatt agtggctccc
     3121 ccggttgggc tcgaaccaac gacctagtga ttaacagtca cccgctctac cgctgagcta
     3181 caggggagtt tgcattccat ggaacgcgga gggattttta ctggaggtac gggcgtttgg
     3241 caagtacttt tttactcttg caggcttttt gacgcaaaaa ggcttccgcg gcgcggtcat
     3301 ggcaacggtg ttttcttgac ctttgaaggg agaatgacca gactttcagc gacatgagtg
     3361 aacatcacca tcatattgct gtcttggccg gcgacggcat cgggcccgag gtcatggcac
     3421 aggctctgaa ggttctggat gccgtaagcg gcaagtttgg ctttaccgta agccgcaagg
     3481 aggcatttgt agggggtgcg ggcattgacc actgcggcaa ggctcttccc gaagaaacca
     3541 ttcgcgcctg cgaagaggcg gacgccgttc tgttcggctc cgtgggcggt cccaaatggg
     3601 agcatctccc cgccaatgaa caaccggaac gcggagccct gctgcctctg agaaaacatt
     3661 tcggcttgta tgccaacctg cgtccgggcg tatgccttcc ggccttgacc catgcctccc
     3721 ccatcaagaa tgaactgatt gaaggcggat tcgatatttt atgcgtccgg gaattgactg
     3781 gcggcctgta ttttggccag ccccgttttc gcgaacggga gggagacgac gaagtcgtcg
     3841 tggataccat gcgctaccat aaaagcgaga tggtgcgcat cgccaaagtg gcgttcgagg
     3901 ctgcgcgcgg ccgccgcaag cgcgtcacca gcgtggacaa agccaatgtg ctgaccaatt
     3961 ccctgctgtg gcgcgagaca atgattgaag tttccaagga ttatcccgac gtggaattgc
     4021 tgcatatgta cgtggacaat gcagccatgc agctggtgcg caatccccgc cagttcgacg
     4081 tgctggtgac ggaaaacctg ttcggggaca ttctttccga tgaaatggcc atgatttgcg
     4141 gttccctggg catgctgccc agcgccagcc tgtgccaggg cgcacaggac aacggcctgt
     4201 tcttcggcct ttacgaaccc tccggaggct ccgctccgga tatcgccggc aagggcattg
     4261 caaacccgat cgcccagatt ctttcccttt ccatgctgtt gcgctactcc ctgggagaaa
     4321 aaacagcggc agacgccatt gactccgccg ttcgccgcgt gattgaccag ggctgccgca
     4381 cgggcgacct ggctacggga gctcccggag aaatccgcgt gaatacggcg gaaatgggtg
     4441 acgccatcat cgccgccctg taaacagggc cgttcttccg gcgccgcagg ggaagggaca
     4501 cctgcggcac gcctttcctc cggggggagc ggtaccgcct cccctccgct ttcctctttc
     4561 atttccggga ttttccggtt ctttatccat ctatcatgaa catccttatc atcggccagg
     4621 gaattgccgg aagttgtctg gcgtgggaat tgaaacgccg cggcgcggag tttacggttg
     4681 cagaccgccc cgtagcggaa acggcctccc gggtggcggc cggattggta aaccccctga
     4741 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn aggaagaatg cctagcggcg gcagaggcct tttacccgga aacggaacgg
     4861 gaattaggcg ggtcctggtg gcagaaaact ccgattttcc gggaagtgga gacggaggac
     4921 cagtttgaaa tctggcagga acgccagacg gctccggaat cctgcgccta tgccggacct
     4981 ctttttccat ggccggaaca ctgggaaggc cgcgggaaag ccgcctatac gcgaggttcc
     5041 gccgtgctgc acgtggaaaa catggtcact gccatgcggc gtttttttat ggaacagggc
     5101 cgttttgtgg aagcggatgt atcccccaca gatatccggc cggatgcaaa cggtcttttc
     5161 cattggaacg gtcgcacgtt ttcccacata gtctggtgta ccggctggga agccgggctt
     5221 catccggaca tgtctccgct gaaggggcgc ccctccaaag gaaccattct ggacttggac
     5281 ttgaaagagc tggactggca tgccggcatc ctgcatttcg gacactggct ggttcataac
     5341 ggttctttct ggcgcttcgg ggccacctac gcgtgggcgt gggatgctcc cggcattccg
     5401 gaagcccccg ccgtccagga attgatgctg gaccttgtta aacgctattc cgggaacatg
     5461 gacgtcatcc gcgcccgtgc cgccgtccgc cccatcatcc gccgcagcca gccggtcgcc
     5521 ggccccattc ccgggctgga cggccagttc gtcttctccg ggctgggcag caagggggtg
     5581 accacttccc cctggtccgc cgcccagctt gccggacatc tcgtgtgcgg aacggatctg
     5641 ccgccggatc tgctgcctgc cgcgctctgg aaatgatgcg gaacatcagc cgtccttcct
     5701 tgaacggaac agctattttc cttgtcaaaa gattctgaac gcgattacat gcggcctaat
     5761 atgcgagcgg agaacgaaaa ctggggagat acggtacgga atcatctgtt tgaccagcgc
     5821 agcgccgtca tggtgcactg gctgattctg ctcaatgtag tcatctttat cctgggattc
     5881 ttcttccagg taaacatccc aaggaacatt tacccgcctg tccatttgga cttgatccag
     5941 ctttacggag cttacagcga atatacgtgc tttcacgagg gggaattatg gcggttgctt
     6001 acctatcagt ttcttcacgc caatctgggg cacatcatgt tcaacatgat tgccctgtgg
     6061 tttttcggcc cggttgtaga agaacgtttc ggccattggc gcttcctgct gtattacctg
     6121 ttttgcggcg tggctgcagc cctgttttcc tccctgctgg ggtacatggg attttttgat
     6181 ccggaatggc gcttcattcc catggtgggg gcctcaggtt ccatttacgg catcatggcc
     6241 gcatgcgcgg tgctctttcc ccatgcccgg gtacagcttc tgtttcctcc ggttaatttg
     6301 agcgtgcgcc agtttgccct ggccgttctc ggagtcgccg cagccgtcat tatcttccaa
     6361 tggaacaatg cgggcgggga agccggccat ctgggcggca tgttcgccgg atttattctg
     6421 acattgctga ttttatggaa ggaaaagctg tcttccccaa gacggaaagc tgcctcttct
     6481 tcccactatt cccctgagcc ttccagccgc cgcccggcac aggcttaccc ctctgaaaag
     6541 gaggtggatg aaatcatgga aaaaatatcc aggtccggcc tgtccagcct gacggaggag
     6601 gaacgggaaa ttctccggcg cgcttcccgg cgctgaacta tttgttagat atttttatat
     6661 ttttcgttat tttccttcct ttttgccatc catttcctag catggatgaa tgatgacgcg
     6721 actgtattcc tccaccgtcc tgggcgtaga tggcgtagaa gtggaggtgg aagtggattt
     6781 ccgccccatg gcggaaaaac gcacctttat tgtcggcctt ccggatgcgg ccgtgaaaga
     6841 aagcgggcag cgtgtggaca cggccataca aaacagcggc cttccctttc agcagggtat
     6901 cttcgtcgtt aatctggctc cggcggattt aaggaagcag ggccccggat tcgaccttcc
     6961 catcgccctg ggaatggttg cgggagcggc tgaaaaggag atggacgctt ctgcctggtg
     7021 cattgtggga gaactggccc tggacggaac cgtacgcccc gtgcaaggca ttctgccgca
     7081 aatcatggaa gcgcggcgca tgggcaggaa gcgcatcatg ctgccgcaag ccaatgccta
     7141 tgaagggacg cccgtacagg gtgtggagat ttatcccgtt tcttccctga gagaagcctg
     7201 gcatttgctt acttccgacg ccctgccgcc tccatttacg ggttcagggc gagaggccca
     7261 tgctccacgg gacaaagacg ccgctgtgga ttttgacgaa atcaaggggc agccttatgc
     7321 acgccgcgcc atggagattg cagcggcagg aggccataat atcctgcttt gcggctctcc
     7381 ggggtccggc aaatccatgc tggcgcaaag gctcccgacc attcttccgc ctctgagtcc
     7441 ggaagaagcg ctggagacca gtaaaatcca ctccgtatgc ggtctgctga aacgggggaa
     7501 cggcctggtg gaccagcgtc cgttccgggc tccccaccac accatttccg atgccgggct
     7561 gatgggggga ggtgcgaata tcacgccggg agaagtatcc cttgctcaca acggcgtcct
     7621 ctttctggat gaattgccgg aattccgccg tgccgctctg gaaaccctgc ggcagccgct
     7681 ggaaaccggc caagtagtta tttcccgcgc ttccggcacc atgacatttc cgtgccgttt
     7741 catgttggca gccgccatga atccgtgtcc ctgcggttat ctgggagaca gaagaagagc
     7801 ctgcacctgc cccccggcgc agatcgcacg ctaccgccgc aaaatttcag gcccgctgct
     7861 ggaccggttt gacctgctga tggaagttcc cgccgtggat ccctccattc tggcttccgc
     7921 gcctgccgga gaatgttccg ccagtatccg ggaacgcgtg gcggccgcac ggcggctcca
     7981 gtccagcaga tacgcaggaa cgcctttccg caataatgcg gccctttccg gaaaagcgct
     8041 ccagaagtat tgccgtctgc tgccggaagg gagggctatc ctgctccgcg ccgtggaaga
     8101 actggccctt tccgccagag cctatgaccg cattttaaag gtggcccgaa ccatagcgga
     8161 tttggaagga acttctgata ttcaggatgt ccatttgtat gaagccgtgc agtaccgcgc
     8221 tttcgagcaa tcactgaggg attagcacta tgaatctctc tcctttcgca actaccccaa
     8281 aaacaaagac atattcccgt tccgaaaata aaaaagaggc agcttttcag ctgcctcccg
     8341 aataacgagg atatttacta tgaacgtctg cggcgcaaca gcatagctcc caggcccaac
     8401 aggcccaaag acgctgcggc aggctcggga accgtttcat aggaaacctg tatacctgta
     8461 attgtataag cggaagtagc gcctccagca ccaccgcgta ctccggcaac taccttatac
     8521 ccttcctccc aggtaagggg aaccgccgac atatccaggg aaattgtcgt tgtttccgat
     8581 gtagaggtaa atgaaaagtc ctgtatcttg gaaagttcct caaccagagt gccggttgaa
     8641 tcatatacgg caagcaccat ggaataactg ctgattacag aagaaggagt attgcaggaa
     8701 aacgtaatgg aagaaacgga atccccctcc tgcggccctc caagaaccat ggcaaaggaa
     8761 gaccccgcta ctccggagcg accgcataat gtaatggagc ctacatcatc ctggttaacc
     8821 caggtgcctt cgccgtttac cgaccaattc ccaacactgt tgttattgcc gccggcatat
     8881 attccatttt gcaggccctt tactttgtcc agaagctctt gccccgatgt tctcgccata
     8941 gtggcggtct gtgatacgct ttgcgattca ggcagctcag caacaatcgt agccgcctgg
     9001 gcggacatca tacatacgca actggctaac aaaacataat gataaactct cataacgctt
     9061 tgcattgatt agaggattgt taatccgctc tttttctttc cgtaacatca tggggaaaac
     9121 gctggaccta ttttcttcat gaatggttta cgataacgtt cgattcataa taatgaatga
     9181 tttatgaaat aacatgcaaa tgttagaata gaagaggcgc aaggattagt ctttataaag
     9241 tccttacatc aaaaaagaag tccactgcca gaataccgct ctttcaactt tttatgttga
     9301 caacggatta acaatccgct gtccccttta tccaatcctt aatgataaag aaaaagccgc
     9361 ctcagcaaag tgaggcggct ttttccaaga agacttttta acgaatagct tccaaagcct
     9421 gtttgaggtc cgcaataatg tcttccgcag cttccgtccc tacggaaaac cggatgaggt
     9481 caggccgtac gcccgcctcc atgagctgct catccgtcaa ctggcggtgc gtatggctgg
     9541 cgggatggag aacgcaggta cgggaatccg ccacatgggt cacgatggcg gccagcttca
     9601 aactgtccat gaacttgatg gaacgttcac ggcctccctt aatgccgaag gttaccacac
     9661 cacaagattg gccaccgcgg aattgcttct gcgccaattc ataatactca ttggaagaaa
     9721 gacccggata attaatccag gcaacatctt cctgcccttg caggaattcc gccactttct
     9781 gcgcattttc acaatgccgc agcacacgca ggtggagggt ttccaaaccc aagttgagca
     9841 aaaaggcatt ctgcggagac tggatggaac ccagatcgcg catgagctgg acggtcgcct
     9901 tggtaatata ggctcccttg ccgaagcttt tgctgtagga gagcccgtga taggagggat
     9961 ccggttccgt cagacccggg aatttatcct tgtgcgcttc ccagtcaaag ttgccgctgt
    10021 ccacaatcac gccgcccacg gcggcggcat ggccgtccat gtacttggtg gtggagtggg
    10081 tgacgatatc cgcaccccat tcaaaaggac ggcaattaat gggtgtggca aaggtattgt
    10141 ccacaatgag gggaacgccg tgcgcatggg caatgtccgc cattttacgg atatccagca
    10201 cgcaaagcgt gggattggaa atagtctccg caaagaaagc tttcgtattg gggcggaacg
    10261 ctttggcgat ttcctccgcc ggagcgttct ggtccacgaa agtaacttca atgcccattt
    10321 tccggaatgt gacggcaaag aggttgtatg tgcccccgta aatcgccgaa gcactgacga
    10381 tatgatcccc agcctcgcaa atattgaaaa tggcaaagaa ggaagccgcc tgaccggaag
    10441 acgtcagaat ggcggccaca ccgccctcca gttcggcaat ttttctggca accgcctcat
    10501 tggtggggtt ctggaggcgt gtataaaaga atccggcttc ttccagatca aacaattttg
    10561 ccatttgttc gctggtctcg tacttgaacg tcgtgctctg gtaaatgggc aatacgcggg
    10621 gttcgccctt tttaggcgcc cagcccgcct gcacgcacag ggtctccggt ctcaattcct
    10681 gattcatcat gcggggcatg ctatgcgatg cctcctgcct tgtaaagcag ggaaaaccag
    10741 acgtatggac aaattcgccc ctgcccccgc cgaagcacac tcccggtttt ttcccagtgt
    10801 aaaacccctg ttcccggcaa atcaaccgca aggacagggg cgttttacct gcgcacggga
    10861 taccctacga tcgaatgaaa cggcaattcc tccgatacct ctttcccgga accaaccgtt
    10921 ccccggtgaa taaagccggg catctcacta cttgatttcc ggcaaagggg tggaaatttc
    10981 ctcttccgtc agatagcagc cgggatcgga cccgtaccag tgtccgttgg caaacgcggc
    11041 acgggtgcgg aagcctccgc cgcacagggc gaagtgaacg cagcggccgc aacggccttc
    11101 aatcttcttc tgccgttcca gaggatcgtc ggaaccgcgc agctcaggca acatttccgc
    11161 tgcagctccg gccctggcat cccataaatc ggaaaaccgg tcacttttca cattgcccag
    11221 agtaacggat tgccagaact ggtccggatg cacggcaccc tgtgtgtcaa tattggcgat
    11281 gccccggccg gaactgttcg cgccgccgcc attccattga agcaggccga gggtttcctt
    11341 cgccagaggg gagccttccc ggagctggcg cagaagcagg taaattccgt ccgcaggctg
    11401 ggttacagtc agcacttcac ggttttttcc ctccgccttc cattcctcta cacgggcaat
    11461 cagcgtatcc atggcattcc ttgcttcctc ctgagtcagg gtctgcactt ccacgccgcg
    11521 gccagtgggg accagatggt aaaaacagac gcgctggatg tcgttggcgt caataaaatc
    11581 caaaatctgg cccatgcact gcacattgtt gcgggtcagc gtcaggcgca ggcctgtctt
    11641 ttgccccact tcactgcaaa gcctgaatcc gcgcaccgcc tgctcaaaag aaccttccac
    11701 accgcggaat ttgtcatgaa cggcgccaat gccgtccagg gaaatgccta cgtacgccac
    11761 gcccaattcc ttgaaccgcg ctgcagcttc cggcgtaata cgggtgccgt ttgtggaaat
    11821 ggtcaccttc agcccttttc cagtggcacg ttccagcagt tccataaagc gcggatgcac
    11881 cagcggctcc cctccggaaa aaagtagggc attcaccttg tagtccgcca gatcatcaat
    11941 gaccgcgcaa cactggtccc aatccagctc tcccttaaat ttccgggcag aggcgtctgc
    12001 ataacaatgc acgcatttga gattgcaggt acgcgtaata ttccacacca caatgggctt
    12061 gcgcgcacgg gacgaagccg gtgcacagga ttccacggca ccccctgcaa aacgaccgtg
    12121 cccgtggccc tgtccgtatc tcaaatggtc cgcgggctgt tccgcccctg tccaaagtct
    12181 ggtaatatta atcatggtgt tgtgaaagcc tgggctgata agggcaaagg ttgtcttccg
    12241 ccatcatgtc tccgtaaacg gcataagcgc gagaccggga ccctccgcag atggtacggt
    12301 actcacaccg tccgcatttg ccctgcaacg cattgtcatc ccggagtttg aggaaaaggg
    12361 ggtgcgtgcg gtagatttct ccagggtcat ccgtccggac gtttcctgcc gttatgggga
    12421 gaaaaccgga gggctgtatt tccccggtat gagaaataaa cataatgccc ttgccgtccc
    12481 gggtgggaat ggcgcggcga gcaggcttgc ttccctgagc acgggcttcc tgaagcgcca
    12541 cgcggcgata atggtgccct tccgtcgttt tgacgccgaa agaaacctca tggcttatcc
    12601 tgctcagttt tttccagacg gcttccactt cttccgccgc aggcatctca tccatggcgg
    12661 cgcggcctgt gggcaccaga agaaacacgc tccacatgcc cggtttcatt tttttcacca
    12721 gctccacaaa gtcgtcaaat tctcccagcg tgctcctggt aatggtggtg ttgatttgca
    12781 agtgcatgcc ggcttccttc gccatttccg cagcccggag agtccgttca tatgtgtggg
    12841 ggacaccgcg gaaggcgtca tgggtacttt catgcgcacc gtccaggctc aatgacatgc
    12901 tctgaacccc agcttctttc aacgcatgga aatccgtgtg caacagccgg gcggtagcag
    12961 cagggctcaa tgctacatgc agccccttgc cggaagccgc acggaccaat tccagaatat
    13021 ccggcctcat gaccgggtct ccgcctgtca gcaccagcat gggaggccgc agttccgcca
    13081 actggtcaat caggcgcaat gcctcctcat gagtcaattc atccgggtgg ggacgcggct
    13141 gggcgacggc gcggcaatgt ctgcacgcaa gcgcgcaagc gcgcgtcact tcccagaaga
    13201 ccaggaaagg gcgttcattg agatctgttt cgctaaaaac cgggcgcatt tttcaggaca
    13261 gccaatttag aacaattata attttttgac cagcccaaaa ggacgcatcc tttaaaaagt
    13321 tcacttagcc gcctcttcac ggattttttg aacttcctta ggcagttcgg aaagggaatt
    13381 cagagcttcc ttcaggttcc tggaagcatc cggagaaagc atgatggccg ttacatgctt
    13441 gacaatgggc tgcatcattt ccacaagctg ctgctgcact cccatcagct tggcctgctg
    13501 gttgcgggtg agaggcggca gggcagcctg tttcctggca acgttcagca tagccgcccg
    13561 gtagcgacct atctgttcca cagcagcctt gccggtttct tcatctctta ccctggccag
    13621 agagtcgtta agctttttta cttcctccac agttttggtt agcagggttt catgcgtgtt
    13681 gccctccgca tcctcctgga acggctccgc ctgctcagcc ttatcccggg aagcctgcgc
    13741 cagctccttc tgaacagact gtaaatccgc cagggccttt tgcaaatcgg gcgtaagcag
    13801 accttccttc ttcaaacggg acagagattt tatcaattca cctgaaattt tctgtgcttc
    13861 cgtcatctgg gcggaaacct gagcctgctc ctgaagcgtg gcaccaggaa gttccagctg
    13921 tcttttccca atgtccagca gctttttatg aactttttta accgtttcca cagcagcggc
    13981 cgctccaggt tcatccgtta ccttgtccag agccgctgcg atttcatgca tgctggcaac
    14041 gctctgcctg gtcagggaaa gatgggagtc ctgggcaaat gcggaggctg ttatgcaaac
    14101 ggcagtgccc aggctgatgg aagcaacaat tttattcatg atacggaagc ctttccggta
    14161 tggaagaata cgttttcctc ctttccccgt caatggcggc tattgtcaag accactatat
    14221 gaaaaaaggg ctgtccgcaa acaagcggac agcccttttg aaatcttaac agcccggcct
    14281 tagtcgcgct gctggcgtga atacttgtag tagctctgga agctgagttc cgcagggaat
    14341 tcgctcaggc cgccattggg attgtattcc ttgaggccgc tcatgccgca gcgcccctga
    14401 taagggggct tgtacgtgca tttgtaagta ggagcccaga aggtgacaag ttcataaatt
    14461 ccgtagccca tgcgttcaaa catggtagcg gtgccatcaa caatgcctac ggaaaaaccg
    14521 gcataatctc cctcgcggct cgtccagttg atgattccca gcgggatttc ttcaacggaa
    14581 taaataaggt tggcaatggc gcggcccagc ttgcgggtgg gggtgtattc ggaagcgggg
    14641 ggagcctgaa tgtccgcact ggcgacacca acgacgccgg cggcaaaaag agctgagatg
    14701 atggcacgtt tcatactgat tgtt
//
LOCUS       DAWEMX010000058        14308 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1757_length_14308_cov_12.749246, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000058 DAWEMX010000000
VERSION     DAWEMX010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1757_length_14308_cov_12.749246"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1076..1085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8413..8422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13288..13297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3370 a         4409 c         3654 g         2845 t
ORIGIN      
        1 ccgaatgttc agttcgacat tgtctccaat ccagaattcc tgagggaagg atgcgccgtg
       61 gacgacctgc tgcaccctga ccgcattgtc attggagcca actcggaaca ggccatgaac
      121 gtcatcaagc gcgtttacca gcccattcat gcccccattc tggaaacgga cgtcaactcc
      181 gcggaactca tcaagcacgc agccaactcc ttcttggctc tgaaaatttc ctacattaac
      241 gctgtcgcca aggtctgtga aaaaacgggc gcagacgtgg aactggtggc ggaaggcatc
      301 ggcatggaca aacgcatctc acgccacttt ctcaacgcgg ggctgggcta cggcgggtcc
      361 tgcttcccga aagacgtcaa agccttcatc aacatcagcc gcacgctggg cattcccttc
      421 accctgttgg aggaagtgga acacatcaat gatacccagc atatccactt cctggacaga
      481 attcgggacc gcctgtgggt cctcaaggac aaaaagatag ccgtttgggg ccttgccttc
      541 aaacagaaca cggacgacgt ccgggaatcc atcgccctga aactatgtga aaaactctgc
      601 ggggaaggag ccattgtcac cgctacggac cccaaagcca tgcacacggc ggcccccatt
      661 ctgaacccta tgggggtcaa actggtggaa gacatgtacg aatgcgcccg ggatgcggaa
      721 gttctggtca tcgccacgga atggagcgaa tatgcgaatg cggatctgca gaaactcgcc
      781 ggtgtcatgc gcaaccgcat catttttgac ggaagaaaca tcctctcccc ggcaaacctc
      841 cgggccgtag gatttgaata ccactccgtg ggcagaccct ccgttgaaga agcctgaccg
      901 cctccgggaa ccgtcccggc ttcaaacgtc cccatatgta aaaggcctcc ggtacactca
      961 acgcaccgga ggcctttgtc atgtattcgc taatcctgtt ggccggtcaa aactcaaata
     1021 gcagccccgc acgcaaataa aaaccggggt catagtggta cttttccaaa tcatgnnnnn
     1081 nnnnngtgcc ggaattcccg aaggaaaaac cgacatccgc caccagcctg gggcgcacgc
     1141 tgccccagtg acccaacccc acatcggcgc ctatgccggc gacgccggaa atccatgcaa
     1201 actggcggac gcggcggtca tggtgaatcg tccatgtgcc ggacaccacg gaaacgaagg
     1261 gtccccactt caacccatcg ctcaccttgt tgatatagga aaggcgggcc ccttccagct
     1321 gaagcgtcca attgcgggaa gcttcccatt taaactggat aaggggcagg aaaggataat
     1381 ccccaagctg cggataacag ccgatgccga ttccatagct gaaccggtcg ttcaaggggc
     1441 ctgccagaag aacatggcct cccaagaaaa tattatggct cgtccatgta tcgaaatccg
     1501 tggaaatctg gggcgtcaca ccgaaaacca ggcgcgtctt ccccgtgatg gcataggagg
     1561 cgttcacatt cagccaaatc gtgtacagcc ggtccacatc catctcatcc gctccttttc
     1621 cattaaacca gctcagacgc aaggccgccg tagaagaaaa attccaggaa ccgctggaca
     1681 tcctgcacaa attcaaatcc gtcatccaat tctggatatc cacgcttccg cgcccgtttc
     1741 tggaatccat ggggccaagc aattcaaaat aggactgcgt ggggaaaaca ggcctggagg
     1801 cggattcccc cgccggagcc ggagcgctgt cgtagccgcc ggaataagcc tctgcataaa
     1861 aaccggtatc gtatccgtca gaagaatcgg aatccggata aggctgggta ttcgcctgag
     1921 cggtcaaaac aatgccggac gcaaaaaaga gactcgataa gaatcgaagg agcatgaccg
     1981 gaaagtatac atacgcgcgc acgcggtcaa tcaaaagata tacaaatgac taattaaccg
     2041 cctccattcc caaacagaac cgcaaccata atcatcacga ggacaagaag caagtaccac
     2101 cgggcaatct tgctgccccg gaaacacgct cccgcacaaa caagagaggc ggcaaaacaa
     2161 accgccgcgg ccaaaagcat cacctgcgcc agcaagggaa aatccagctc ccccgcaccc
     2221 gccgccagaa gtgttcccgt cacatacccg ccaaacgacc accccaaccc cgccagccag
     2281 gaaacggaaa atgtcttttg tcggccgggc ctcatcaagc cactctgcaa tccttcttcc
     2341 taatctcaag caaaaaatcc agccttctcg gcaatatctg caaagagggg gcttccccag
     2401 acactcggct cccctcaaaa aaaacaacat cttcgcaaaa cggacagcaa aacactcaat
     2461 cccggttgga aaactcttct tccggcaaag ttgaataaaa acgggataaa ttacttaatc
     2521 cggcattcat caatactcgc gggctccatg aaaagaaaat atcttgcccc ttccatagac
     2581 tttcattata ttacggcaaa accggaaaaa caggaaacga acgtactccc ttccctgccc
     2641 cattggatca agcatcgcac gttacaaacc accagctgaa aaaaacttgt catgaaaata
     2701 aattttttat ggaccgcatg tttagttgcc ataaccggct gtgattcccc ccaaaatcat
     2761 cattctcgaa ttgagagcgg cgaaacaaaa acagaaaaac agttggagaa tttaaatttt
     2821 tacggcactt atgaaggaat tcttcctgct gccgattgtg aaggaatcag gacggcgtta
     2881 acattaaaca acgataacac ttatgtgttg agaagcgaat acataggaga aaaagacgct
     2941 attttcgaat caaaaggctc ttatcatttc atcaatggcc gcctgatgga actggcccaa
     3001 tcctcttcca atgaaaaatc ttattataaa atactcgatg gaagcaaagt aatgctgtca
     3061 gacaaagaag gcacagtcaa tcagggcgtc ctggccgaat attatatact aaaaaagaaa
     3121 taaaactatt tctatcatct attattttct ccattctgcc gcacttccca ccccgcggcg
     3181 gttttcttta caccgttttt cagcttggca cattagcaaa tcttattcat ccgccccgcc
     3241 gtcagaaaaa atccgggccg ccgggaccag gctttcgtca gcacacgggt gttacaagaa
     3301 ctttgcatcg cttttctgtc cgcgttgctt cggacacaaa agatttccgg ctttttttaa
     3361 ctggctgcag gttcggccgc cccgccgcaa caggggtgtt tttcatcttt ccaggcatgc
     3421 cccgcttcca ctccacctat tttacggaca accaccaggg aacagctcaa aaaacaaaac
     3481 ccgtaaatca tttgatccac aggctttaaa atggtcggga agacaggatt cgaacctgcg
     3541 acatcctgct cccaaagcag gcgctccacc aggctgagct acttcccgta taaaaaggag
     3601 ggattagaaa ttgggagaaa aaatggtggc tcgggacggg atcgaaccgc cgacacgggg
     3661 attttcaatc ccctgctcta ccgactgagc taccgagcca cttgcctgca cgcagaacgt
     3721 gcttgcgggg cagaatttat catcctcttc ccaatattgc aaccacaaaa tattattttt
     3781 tcaacttccc tgtattttgc tgcgctccat agcaggaatc cgcccagcca atcaggagga
     3841 agcctgcgcc agaataccgc tcaacagcaa tgggaaaatc agtgccgccc aggatagcgt
     3901 gtgcaggagg aggggaggca tgttcatctt cagcctgtcc agcacaaaca tgaatgcccc
     3961 cgccattgcc agcccataat aaatccacgc atccccggta tattccctgg cggcggcggc
     4021 cagacagaat acgacgtaaa tgactatcac ccccatccat accagggata ccatgtcgcg
     4081 gtcggcggaa tcctgcccgt cctcatcctc ttcttcactc caccgtttgc ggctgaacat
     4141 gcagaggatg accagcgcta ccagatacca ggtgggttcg gaaagctggc ctccccagcc
     4201 gccgcctacc gtcccgtaaa agtaggccgg tattccggca cccgtagcga aggaaacggc
     4261 ggcaaaaaac gcccccatga cgaatccgga agacaggctc cctttctgat gagggttgga
     4321 agagaaatag agaaagagca gactgcagac gccaggcaga aaggcaaatt gaaaaataac
     4381 cacacccaat tggaaaaaca tgatccaaag ccccgtcacg gccgccacca taatgagcag
     4441 ggagataacg aaacaatgct ttttggcaaa aagcaggcat tcatccgtgt agacagcgga
     4501 accgccccgg aatattttca gcacccggtc cagcatattg acgcaccaga cagccacaaa
     4561 caggaattga tacagccagg gggaggaaac taccaccaga tgggactggc tgacggcaaa
     4621 agcccaggca atggcggcag aagggatttc caatcccatc agggaaaacc acgcccacca
     4681 ggggggcctc ttcaacacct gctccatttt tccgtcatat catctccaca accgttcctt
     4741 ccgcaagctc ccgaaccgtc atccacaaac ttccttattt ttacgcgggg atgcttgaca
     4801 tgcaatgaaa catatttata cttgcgttcc ccttgcgtcg gaaacgcaat acatcaccat
     4861 gaagaaagat attcaccccc agtataatcc ggtcgttttt gttgacatca ccaccggacg
     4921 ccgcttcatc acccgttcca ctgttcgttc tcccaagacg gaagttattg acggggttga
     4981 acatttcgtg gtttcctgcg gtatcacctc tgattcccac ccgttcttca ccggcaagaa
     5041 ccagtttgtg gacacggaag gccgcatcga caaattccag aagcgcttcg gcaccgtccg
     5101 ccgcagcgga ggcaagccca agctcaacaa gtaagaactg ccttcagcaa ttttccaacc
     5161 ccgtttcctt cccggaaaac ggggttttta tttgcaggct tcctttgatc tctttttcct
     5221 gattcactcg cagcagcacc atgttgacac cggaccattt tgacagccag tccgcggaac
     5281 tcctccgctc cggccgcccc gtccttgccg gaatcagcgg ggggagggac tccatggctt
     5341 tgctttccct gctgtccggc atgccgggat gcaatctggt tgcatgccat gtaaatcacc
     5401 agctgcgtcc ggaagcggag gaagaggaaa tgtttgtgct ccgttacgcc gcctccctgg
     5461 gtattccctg tgtccgggaa cgcgtcaatg tccgcagtat ggcccatacc cgcggaatag
     5521 ccattgaaga agcggccagg gaagccaggc aggcctcgtt tctgaaatgg gccgccggtt
     5581 acccgggggc tctcgtggct ctggcccacc accggaacga ccagcaggaa acggcccttc
     5641 ttcacctctg ccgcggtgcc tccggcattc acggtatgtt ccccgtctcc acctgggcca
     5701 acggattgac cgtcgtacgc cccatgctgg atttttcccg ggaggatatc acctcctatc
     5761 tggagcatcg ccatattcca tggagggaag attccagcaa ccagtctgcc gaatatacca
     5821 ggaacgccct gcgccatgag attattccca aattgaatgc gctgttccaa agggacacca
     5881 gccttccctt ttcacgtgcc tgccgcctgg aaaatcaaat ccgcacggcg ctttcccagg
     5941 cgctggactc catgaacctg acagatcccc aggggcgcct gttcttgccg aaaatcaacc
     6001 tgcttcctgc ggagctgaga caatgcgccg tgcatgatta cctgcgccgg caacatattc
     6061 cggacctgac ggaagacgcc gtactccggg taatggacat cctgaatacg gacggccctt
     6121 cccgaacttc cctgcccggc ggaaaactgg ccgtacgcaa ggaaaagcgc ctgctggtca
     6181 cggacacgcc gcgcccggtc aataaagcgg agcaaccccc tgcggataca acaggtggaa
     6241 tttaatggct tcattcccca ccaccagcgc agtaccgaac atcctgtctt catccgtcag
     6301 ccgcggcagc tgggcaatcg ggaagagcag accgcccaga ataaggccat agttgtatac
     6361 ggacggcatc atgcacaaca gttttttgga gatatccgca gcatccagag tcgggaagac
     6421 ccaaacctca cttggctgaa gcatggagtt gcggtgcacg cgcacgctgt aggaatctga
     6481 cgacagagcg gcatcaatct ggatttcccc ctctatggag atttcattgt tcaggcaatc
     6541 cttctgcacc atgctcttcg ccagcgccgc agcggccctg gtgcggtccg cagccagttc
     6601 cggaacggag cccgaagtgg acgcgctcag catggcgacg cgaacgcttt tacccagcat
     6661 gtggcgggcc attttggccg tctctacggc aaaataggcc atattttcca ccgtgggttc
     6721 agggtttacc ccggtatccg ccagaaaata gacgccgcgg ccgccgaatt tcttggaaaa
     6781 ttcgggaacg agcacaatgg aaatggcaaa caggggcttg gtgggaacgg gatcctgcct
     6841 gtacttgttt actgcgcgga aaacggaagc cacccttttc atgttcccgg ccacaatggc
     6901 gtccgcctgg ccgtagagca ccatcatggc ggcaaaatga accggttcgg aaacaatttc
     6961 acgggtattc ataggcaacc cgttgccgaa catctgctcc gcccgttcat aacggtcgca
     7021 gaacatctga agatccgagc ttttttccgg ttcaatgata cggacgaatt tcagggaaat
     7081 tccgttcatt tccgccattc tcgtgatgac ttccctcctc cccagcagga taggcgcgcc
     7141 ggcctgctct tccacaaagc gctgggacac gcgcagcacg cggacatcct ccccttccgg
     7201 aaaaacaatg cgcttggggt ggcgcttcag gttatccagg agcggcccag taaaatcatt
     7261 cagcggggaa aagccttgcc agtcactcat atgtcacgtg tcctatttaa cgcaactcta
     7321 aaaaatcctg tccataaaat caactcataa agtcaaagac ggcaaataat gaacaaagac
     7381 atccctccgg gttgaaccgt ccgctccgcc atgctagcat gggcattccc ctccatgtac
     7441 tgcgattacc atacccatac ccccctgtgc ctgcacgcct ccggcactcc gcaggaatat
     7501 gtgcaggcag ccgtccgagc cggactccgg gaatacggca tttcagacca tgccccgatg
     7561 cctggggaac cttttgacga ctggcgcatg aagcaggcgg acatgccggc ctacctggac
     7621 tggctcacgg aagcccgtga atgcgccgcg ctgcacggac tgacggtccg ttccgctctg
     7681 gaatgcgact ggtttcccgg catcgaacca tggatagagc acctgcaggg cctgcacgca
     7741 tgggattacc tgatcggttc cgtccattat ctgggggaaa aggaggaatt cgacaatccc
     7801 cacagaatgg atttctggca ccggacggat gtggaggacg catgggaaca gtattgggaa
     7861 cgcttccgga acatggccgc ctccggaatg ttccacatca tggggcacgc ggacctgatt
     7921 aaaaaattcg gcttccgccc ttctggagac ctgcgccgtt attatgaacc gtcgctggaa
     7981 gccatgcggg aaaccggagc ctgcctggaa cttaacacag ccggttggca caacaagtgc
     8041 ggggaacaat atccggacgg gcaattcctg aaaatggcgg cggaggcggg aattcccctc
     8101 accatcagtt cagacgccca caggccggag gatgtagggc gggatttcaa gcgggccggg
     8161 gaactggccc ggcaggcagg gttcaggcat ctggcctcct tccagggggg ccgcatgaaa
     8221 ctgttccccc tgcccggaat ctgatgcttt ccctctgcgg caaaggaatg ttccggcagg
     8281 ccatggcggc agccgcacgg gcggcctgtt tcccgtattc ctttccggca gtcccttttt
     8341 ccagtaaacg gcacaggccc atggacagaa aagggaatgc tccggagcat ctttaacttc
     8401 tgtacaaact cgnnnnnnnn nngcggaggc acgggacggg gagggggcgg ttggggccgg
     8461 ataaagacag gcggcggcgg ataattccac gtcctgataa tcggagggac aatgatgcgt
     8521 tccgttctcg gctggctggc cacctccctc acttccccct gcaattggga aacctgctta
     8581 ttcaaatccg caatcttctt ctgagtaccg tctttcaagg ccaggtattt cttctccaaa
     8641 tcctgccaat actgttccgc ggtttccctt tttttacgga tggcgcttat ctgctcctga
     8701 taggctgccg cccttgcgcg gaaaacgcgg gcgtcttccg gcaaacccac acggtccgct
     8761 tccagggcat tccactgcag agccataacc tccgcctcca ccttggtaag ttcccccatc
     8821 agcaaaaaca tgcgggaaat atccgcccct tccaggtagc ggggatgccg ctccgcctca
     8881 gacagggact cccggatctt cctggcttct tcatcggccg ccttcctggc ttcttccgcc
     8941 ttctttctgc gcgccgcttc ctccatcctt cgctggtcca gcctggcctt cacttcatcc
     9001 ggcgtcatcc tgtaatccct ttgctgggca tccgtcaaat ctttatagga aaccttggcc
     9061 accccgtccg tgtactggat attgacaaaa tcatctccgg aagacactac aacggcgtct
     9121 ttcagcaccc tgccggaccg cagggacata ttttccgcca gggcggcagg agccaggagg
     9181 cataaaatca acaaacgggt tcctgttttc atatgattaa cctcttctac gttggtaaga
     9241 ggcgcaagga aaggaattta ttcaaaaaaa taggaccaga ggttaatact ggcgcagccg
     9301 ccggttttta gctagtatat ggcgagtcat ttacacgtat tatgagccgc tattcttcat
     9361 cctggacacc tttacccgcc cttcccgatc cggaaggctt cgccggcatg tacgcaggga
     9421 cctgcggaga aataatgatt tgtgcaggag gtaccaattt cccggaaaag cccatgcttg
     9481 aagggggagc caaaacgtgg acggaccgta tctttacgct ctctccgggg gaaaacgaat
     9541 ggaaagaggc cggcacattg cccgcgcctt atgcatacgg cgcctccgcc ggcatcaggg
     9601 aaggcctgct gtgcatcggc ggctgcggca aagaagggca ccggaaagac gtttacctgc
     9661 tgaacctcgc ggagggaact gtcaccacgc atcctttccc ttccctcccc atctccctgg
     9721 catatacggc ggctgccgtc ctggacggga aagtctatct tacaggaggg tgcgaacgcc
     9781 ccggcgagca ggactgcact aaccgggtct tcatgctgga cacacgccgg acggaacagg
     9841 gatggctgga acaggccccg ctgccgggcc gcggccgctt cctgcatcag atggccgcaa
     9901 cagacggagc cctgtacgtg ctcggcggca tcggcctgcg ggaacaggac ggccaacagg
     9961 tccgggaact gctgaaggaa gcctggagct atactccgga acacggatgg acgcgcctgc
    10021 cggacatgcc ttacgccatc gccgcagccc ccaccccggc cccggtttcc ggaaacggcg
    10081 tcatttacct gcttgggggg gatgacggct ccggaaaaaa atacactccc caaactaatc
    10141 ccggcttcaa taaccaatcc ctgtgcctag acacggcaga catgacatgg cacgacgcag
    10201 gccccatcgc cgcgcccaga gctgtccttc cgtgttgcgc gtggcaggac cgtttcgcgg
    10261 ccgtgaacgg agagctcaaa cccggcaggc gctctcctga ggtatggagc atctccctcc
    10321 cctgatttga ccggaatcca attaaacctc tcaacatatg cacatgacac gcacccttac
    10381 ccgaatttcc aaggcttgcg gctctgctct ggcagccctg cttctcatcg cggctccggc
    10441 cgctgacgcc tccttttccg tggatgtccg gacgtccgga actgttcccg accttccgga
    10501 cccggcaggc cgcgccggca tggttgccgg aaccgtcacg gaagacgacg gcagccagtc
    10561 cgtcatcgcc gcagggggcg ccaacttccc ccaggctgcg ccgggcgcct caactccgga
    10621 ggaacgcggc cccaaaacct actaccagga tattttcaag cttcgcaacg gccagtggag
    10681 caaggcggga acattgcccg tcccgctggg ttacgccgcc tttgccagcg tgggcaaggg
    10741 gctggctgtg gccggaggcc acaatgctca gggcatcctg aaagatgccc tgctgattaa
    10801 aacggacggc tccgtagaaa agcttcctcc ccttcccgtc cccgtaacgg aagcctcctg
    10861 cgccgcccac gggaacaaat tgttcgtcat cggcggcaga gaccgggagc agccggaaac
    10921 cgcactcaat accatctaca tgctggacac cacgccggac acggataaaa tgaaatgggt
    10981 ttcccttccc ccttttcccg gagaggggcg cattctctcc accgctgccg tctgcgacag
    11041 caccctgttc atcataggcg gctgttccct gtccagggat aactccggag aaacctccag
    11101 gacgtatctt tccgacatga tcggctacga catgacgtcc aaggaccctt ccaaatgggg
    11161 ctcctccggc aggcagcagc ttgccggacc gggaatgccc gtagccgccg ccgcgggtcc
    11221 cgccccggtg cgggaaaact ccattctcct gatcggcgga gacaagcgcg gcaactcgcc
    11281 ggacccgtcc aggcctgtgg cgcagtcccg ggatatcctg gtctatgacg tcatcggaaa
    11341 cacatggacg cgccaggggg aatggcccgt gggcatcgcc acggttcccg ccgttgtcag
    11401 gggctctgaa atcatgacca tcagcgggga aacggccccg ggcgtccgga ctcccgtaaa
    11461 cgcctccgcc tccgcaggtt accactttga aatgagtacg gttgattacg ccgttctcat
    11521 tctgaccatc atcgtcctgg cgatcatcat cgtctccgcc gtgcgcaacg gcgttaaaaa
    11581 tgttgcctcc gtcacggacc cgaacaccaa gccgggcctg tgggcctggg tagccgtcat
    11641 tgtcctgtgg tttgtggtca tgctgaacta ttttgaccgc cagctccttt ccgccctgca
    11701 tgaacccatc gtacgggaca ttccgcagac ggaagcccag ttcggcatgg tcacctccgt
    11761 tttcctgctg atttacgccc tgctcagccc ggtgggcggc tttctggcgg accgctacag
    11821 ccgccggctg atgatcctct gctctctcgt cgtctggtcc gtggtcacat ggtggaccgg
    11881 ccatgcggaa gattacactt ccctgctcat tgcgcgcgga gcaatgggca tcagcgaggc
    11941 attctacatc cccgccgccc tggccctgat tacggactac caccgcggca gcacgcgctc
    12001 catcgctacg ggcctgcaca tgagcggcat ctatgtaggc atggccgtcg ccggtttcgg
    12061 tgccaccatg gcttcatgga cgggatggcg catgacattc gccctgttcg gcctcatcgg
    12121 cgtggcgtac gccgtcatcc tcatcctgtt cctgaaagat ccgtccaaag ccccggcgga
    12181 tacggcccag gcaaaaaaac cgtccgtacc ggagaaaaaa acggtgctgc tcaatgtgga
    12241 caatgacgaa cagaccatca aggaaccctc ttccaaactt tccaccggag ccgtcctttc
    12301 cagccttctg agcggacgcc ccatgtggat gctactggcc gtagtggcct ttgccggagc
    12361 cggaaactgg ttccttctca cctggtatcc caccctgctt caggacaaat accagctctc
    12421 ctccgcggaa gcgggccccg ccgccaccct gtggagctcc gtggccaaat acgtcgccgt
    12481 gctgggaggc gccatcctgg cggacatgtg gtacaggcgc aacgcccgcg cccgcgccct
    12541 ggtgccgggc atcaccttca ccatatccgg cccattggtg gttctggcgc tgctgcccgg
    12601 catcttcggc tgggacatca ccgtcccgct cgtgctcatg ctgggtctgg tcgccacgca
    12661 ggggctggcg cagggatcac tggacgccac cctcatgcct gtgctacgct cccatatcga
    12721 cgagcgttat tccgcaaccg gctatggcct cctcaacctc acatccgccg gcgtgggcgc
    12781 cctcatctcc ttctttgggg gatggttcaa ggatcagggc gttccgctga ccaccaccct
    12841 ggcggcggca ggatgcctga tgctgttctg cgggctgctg ctcctgatgc tgccgcgtcc
    12901 caaacattaa acgcaattat gttcatgcct cccgtcttac ccctgatgaa aaagcccgca
    12961 cgcctctgcc tggcggcggg aactctgttc gcccttttcc ttacccccct tgcacagggg
    13021 cgcacctgga ccaacctcca agggaaaaaa ctggaagcag aattcatcag gctggacggc
    13081 caaaaagccg tgctgaaacg ttccggcgga caaaccgtct ccattcccct ctcccagctc
    13141 tcccgggaag acagggattt catcgcggaa caggaaaaag gtggggcact cccctccaat
    13201 acggcggaca attaccacct gccgtggccc aggagcgtca aatgcccgga caatttcaag
    13261 gtggaaacca tcaaggagga accgggannn nnnnnnngaa ccgggagaat atatttatga
    13321 aacaccccat ttccgcttca tctgcgacgc caagctgggc accggcatga tcaagcgcct
    13381 gggcctcctc tttgaggcca cccacttggc caacaaaacc cttcctatag gaaactcccc
    13441 tgcccatgac gattccgcca aattccccgc ctacctgtat gaaaaattca gcacctatct
    13501 ggaaaacggc ggacgcgaag gcacggcggg catcttcctg gggacaacgc ggccagggga
    13561 ccgcggaaga attctggttc cgttcgattc cctgggagtc aaaaccatgg gaagcacata
    13621 cgtcattgac cgtgacaagg acgcttccac cctcatccat gaactgacgc accagctcat
    13681 gtcttcgcag gccaagcagg ccagctggtt ttgtgaaggc tccgcggaat acatgggcat
    13741 gacgccctat gccggtggcc gcttcaactt tggagccaac cgatcccaca ttgtctcccg
    13801 cgtgacggaa tacggcaaaa aaaatacggg gggacgggcc cttggggatg actttgaggc
    13861 acccggcctg gaagcttaca tgaacatgcc ctattcccag ttcacgggag aaaacgccaa
    13921 cctgaactac ggcctggccg ccctgatggc ctactatttt taccacatgg acggcaaggg
    13981 cgatgcccgg cgcatcaaga attacatgaa agccattcaa tccggaacca gtgaaaagga
    14041 agctcagaaa ctcctccttg acggacggag ctatgaagaa ctggccaaag aaattgaaca
    14101 gaaatggcgc aaggccggcg ttaaaatccg cttccgttcc tcttcctgaa ctatgccgcg
    14161 gcgtgccgtg gaaacagtcc cccgacggaa aacgaagggc ggtttgtgcc ctgaaaaacc
    14221 gttgacacgg gagggaaagg cggcacactt ccctcaacag ggattcacag acacgcatgg
    14281 cagaagaagc ttccatatcc gccctgat
//
LOCUS       DAWEMX010000059        13779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1841_length_13779_cov_10.632760, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000059 DAWEMX010000000
VERSION     DAWEMX010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_13779_cov_10.632760"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2807 a         3540 c         4149 g         3283 t
ORIGIN      
        1 ccgggaggtc ttctcatgct cccttcttca ggattcttgc cggaaatgga tgttttcggg
       61 gaggccgtat ggtggaatcg cggagggaaa gaagttgttt cctgaaagcc tgcggcatcg
      121 gaaaggaacg cctggaaaag agggaaaatt tgtttccaga ccctcttgtt tatgggaatg
      181 gaaggctgag atccggaaaa gattaagggg ggtaagtttc ccgatgggcg cgtaggccct
      241 tttcacatga ttggccggca tggtctccgg gcatatattg cggtgaaatg agtttagtat
      301 gaatgtaaaa aacagaaaaa caaagaaaaa tgttgaacat tcttaaactc aatgcatttt
      361 tggaatctcc aaaatatgtt agagtttcgt gcggaacggc tgcatgaggc ttcgtaaatc
      421 caatgtgaaa actcgttagt catgatatac cgttttaaga aaacattcat cggcgtggga
      481 gggcgtgaat acattagcaa tcccaaattg agcttcccgg aacatgtcaa aatccgccgc
      541 cgcggaacga atcccaggtc cggccagcct tacacggagg cgggtagcgc atttgtggag
      601 acatccgccg tcgcggcacg gctctgctgc agcgcttccg ccgcgcgcca gtatctgcac
      661 cgccacggtg tccgtttccg ggtggtccgc aagggtacgg gccccctggc cctgtactgg
      721 cggcggctgg atgtggagcg ggttcttgct ggccgggagc ccgtgcgccg ccgcgtgcct
      781 ccgtattata tggatatggc cggggctgtc agcatgctgg gagtagtgcg cagctatgtt
      841 tacaggctgg tgaaaaaagg gctgctgcgt gaatacaggt gcaggatgaa aacagcccgt
      901 ggactgcgcg tgcgctgctt ttaccaccgg gaggatctgg aacatgtcag gctgcacgtg
      961 ctccagaaaa gatgacggaa gcggttccgg aggatgcgtt tttggcttgc gcgcaggccg
     1021 gattgtgtca cgttaagggt atgatagctt ttctgcgcgg cacggtggcg gaatcatatc
     1081 cccagcggtt gatcctggat gttcatgggg tggggtatga agtgattgtg cccctgtcca
     1141 cgtttgacag gctgaatccc cttcccggca gggagctgac gctgaaaacc tacctgcatg
     1201 tccgggagaa catgcaggtg ctgtatggtt ttgccacgga tgcggaaagg gatattttcc
     1261 tgctgctgat tgagcgtgtt tccggaattg gcccggcaac ggcgattgcc attttgggtt
     1321 ccctgtccgt ggagcagttc aagcaggcgg ttgtgggcgg agacgtttcc ggcattgcgc
     1381 gggccaaggg ggtgggcaaa aagacggcgg aacgcatcgt cctggaactg aaggataaag
     1441 tgggcctggc cgccacgtgg gaagcccagg cccagggcgc tacttcccag gccgccgggg
     1501 acgcggaatt ggcactgatt gcgctcgggt tcaagcaggt ggaatcccgc aaggcgatag
     1561 ccgcccatct gaaggaccat ccggatgcgg acgtggacga gctgatacgc gccgctttgc
     1621 gctccatgaa ttgaaggagc ttccggagga aggagggcgt ttttacaagt ttttgatttt
     1681 catgaaggag tatgtacgtc tggcggagtg ttccctgttt gcggaagctg gggaacatac
     1741 tgccatgatg ggggaacaat agtgttgacg gctggttgaa acgtttttaa acttgcaacg
     1801 tccatggtac ttgaataagt ccgggactag taattaatca aatctgaagc cctcaatctt
     1861 atggccaaat acgctattaa cggttttgga cgcattggtc gcaacgtact gcgcgccatg
     1921 tccaaggaag aacgcaacaa ggttgttgcc atcaatgacc tgactcctat cgaaacgatc
     1981 gcccacctgc tcaagtatga ctccacgcag ggcaagtttg acggtgaaat ttccatcgag
     2041 ggtgattatc tggtcgttga cggtcacaag atcctcatca ccgtggaacg tgatcccgcc
     2101 aaccttccct ggaaggatct gggcgtggac gtcgttctgg aatccaccgg cctgttcacc
     2161 aagcgcgacg ccgccaagaa gcaccttgac gccggcgcca agaaggttct tatttccgcc
     2221 ccctccccgg atccggacct gactttcgtt ctgggcatca acgacagcga atacgatcct
     2281 gccaagcacg acatcgtttc caacgcttcc tgcaccacca actgccttgc tccgatggtg
     2341 aaggtgctgg acgacaagtt cggcgttgaa aagggcatga tgagcacgat tcactcctac
     2401 acgaacgacc agcgcattct ggaccttccg cacaaggatc cccgccgtgc ccgcgccgcc
     2461 gcgatcaaca tcattccgac gaccaccggc gccgccaagg ccattggtga agtaatgccg
     2521 aacctgaagg gctccctgaa cggcgcttcc ttccgcgttc cgactccgac cggttccctg
     2581 accgactttg tggccgtgct caagaaggat gtgaccgtgg aagaagtaaa cgccgccatg
     2641 aaggaagccg ctgaaggccc gctgaagggc attctggctt actccgaaga agcgctcgtt
     2701 cttcaggaca tcgtttccga cccccactcc tgcatctttg actccggctt cacgtatgtg
     2761 gtcggcggca acctggtgaa ggtctgcggc tggtacgaca acgaatgggg ttactccaac
     2821 cgcgctgccc aggccatgaa gaagctgggc gacagcttgg gctgcggctg ctcctgcggc
     2881 aagtaagttc tttcggacaa cgcttgctct tcagttgcac gacaagtctt gccgtccgcc
     2941 tgtttcaact ttccggcttt cctggccggg tttgccgggc gggcggggat ttatccggga
     3001 gggcagaaat gccctccctt ttttattcat tgaaccagac aatataaacc gatatggcta
     3061 aacttactgt tcgcgacctt gacgccaagg gaaaggaagt cctcatgcgc gtggatttca
     3121 acgtccctct caaggatggg gaaattacca atgacgcccg cattgtggct gctctcccca
     3181 ccatcaagtt cctgctgggc cagggcgcac gcctcgtcct cacctctcat ctgggccgtc
     3241 ccaaaaacga acccgatccc gctttttccc tgaagcccgt tgccgcccgc ctttccgaat
     3301 tgctcggcaa ggacgttaaa ttcgttcccg ccgccattgg cccggaagcg gaagctgccc
     3361 gcgccgccat gaaggacggt gatgtggttc tgctggaaaa tgtccgtttt tatcccggag
     3421 aaaagaagaa cgatccggag ttcgccaagt ccctgctggg caatgccacg ctgttcgtga
     3481 acgacgcatt cggcacggcc caccgtgccc acgcttccac ggaaggcgtg acgcattttg
     3541 cggaaaagag cgccatgggc ttcctgattg aacgtgaact ggaatacctg gaaggtaagc
     3601 tggaaaaccc ggaaaaaccc tttgtggtga ttatgggcgg cgccaaggtg tccgacaaga
     3661 ttgaagtgct ttccaaactg atggagaagg ctgatacgtt cctgattggc ggcgccatgg
     3721 ccaatacgtt tctggcggct gaagggtatg acctgggcgc ttccaagatt gaaggggaca
     3781 agctggatct ggctcgggaa attctggcgg cggccaaggc caagggcgtg aaattcctgc
     3841 ttcctgcaga tgtgcgcgta gccatgaaat ttgaagacgg cgccgaaacc ttctgcacgg
     3901 ctcctttcgc tgaaggcggc aaggttccgg aaggcggcat ggccatcgac atcggcgata
     3961 aagccattga agagttctcc gccatcatca aggatgccaa gaccgttctg tggaacggcc
     4021 cgatgggcgt gtttgaaatg gactgctttg ccaagggaac caaggaagtc gcggaagccc
     4081 tggcggattc caccgccatt tccatcgtag gcggcggcga ttccgtgacg gcggccaaga
     4141 agttcaaggt gcaggataaa ctttccttct gttccacggg cggcggcgct tccctggaac
     4201 tgctggaagg caaagttctt cccggtgtgg gggccttgac cgacaagtgc tgctgcggca
     4261 agtaaccgtt catggccggg cggattgttt tcgcccggca ttcagaatgg gcttttttat
     4321 tccccgcacc cgttttcggg tgcgggattt ttgatttgcc ggcttccgtt gaaaaagcgc
     4381 gggagctttg cggttccggc tttttgaaga aggtgggaat ccatgctcat cccgtctttc
     4441 cgttccggaa aggaaggcag tctttttttg ggcttgctct taatatggtc cccggtagtc
     4501 tgagcatgat gaaactgttg cattgtttcc tggctgtcct ttggtggtgt tctcctgtac
     4561 cggctgcgga tattcccgat gcggagaatg cggaatatgg cgggtatccc gggcggtaca
     4621 tgcttttgga ttcttcctcg cccgaccggc agagcctgtc cactcccgta gcggcctatg
     4681 tccatgacaa tgggatggaa gtagctctca ttggtgctat tcatgtggcg gaaccggaat
     4741 attacgaccg gctgaataag ctgttccgca ggtacgatgt gctgcttttt gaaatgattg
     4801 gaggagaggg cctccggcgg gaagaggaat tgcgccgcaa gatagaccgc agcaagccgc
     4861 tgggggggct gactctggaa gaggcgcggg aatggaaccg gatagtggaa tggaggaaga
     4921 aatgcgagca ggaggaaaag tcttttttaa tggggctgct ggggggcgcc tacaaggagt
     4981 tgagcgacat gctgggactc cagacccagc atcagggtat tgattattcc gcagcccatt
     5041 ttgtccatgc ggacatgacg ctggatgaat tccgcaaggc gcaggccaga aagggtgaaa
     5101 gcattgccgg cctgatgctt aaatccgtct tgtcctccct ggttgagaaa accgggacga
     5161 accgggccgg tgaatttgga ttgatggcgg atttcctggc cggcaataaa acggggttga
     5221 agaataagct gatggtcatg atggccaatg ctcctaatgg tctggagaat accgtgattc
     5281 tggaaggccg gaatgccaag tgcatggaag tgtttgaccg ttggagcggc aagggtgtgc
     5341 gcagaatagg cgtcttttac ggagcggcgc atcttcccgg cctgcacggt gctcttttgg
     5401 aacgcggcta ccgtctccgg gaggtgcgct ggcttccggc gtggagtacc agggagaagg
     5461 gtgcggacgg gcagcgcgga gagggctgag ttttttactc ttttttcgag acgggaggtg
     5521 tagcggcgga tgaacatggt tttccgccgc tccgccttta aagagggaag aagagggggg
     5581 cgttttttcc ctgcctgggg caggatgccg ttctctaggg ggcaggatgc cgcgttccgg
     5641 agggaaccat gcggacgatt cctccgcctt tgatgcgcat ggtgaaggaa agggcatttt
     5701 cggaggtgac tttccgggtt tccacgctcc atccgtcatt tttttctgcc agatcccgga
     5761 gaatggtggc ttggtatgcc gtgtttttgt ccaggaaatt caaaggaatg gaaacggtgc
     5821 gttccctgcc gtctccgttc atcagagcaa tgtaccactg gtttcctgac cgccgggcga
     5881 aggcggcgca ttccccaatg gcagacggcg gcaggacgat ggtttcctcc catacggaag
     5941 ggatgttccg gacaacttcc cgttcagggg aatccttcgg gaaagcagct tccatgtccg
     6001 cagggttgga gacccagtga agaatggggg acgtataaat gatattggcc gccatttgga
     6061 gggtccagga agagccgcgc agcttggggc cgtggccaaa atagcctccc gtgaaatcgg
     6121 cgtggccgga gaccaggcgg gtaaagggca gcgcgcagta gtgctgcccg ccaatggcgg
     6181 cccagatgtt ctgttctccg ccgtaaatgc cttcccggct catttcgtgc ggccaggaac
     6241 gttcctcccc cgtgggggta ttggctccgt ggaagttgac cataagcttg tttttcgcca
     6301 ggttttccgc tacggcgtcg tagaagtcca ggcgttcctg agaggcggag tccatgaagt
     6361 ctatcttgat tcccctgatt cccgttttgg agagtgagct gaaaaagcgg cgcatatttt
     6421 cccagtcatt ggcggggttg ttgacatctg accatcgcac ccatacccag atgcctacgt
     6481 ttttgccggc ggcgtagctg gccagtttgg ccagcttgtc catgctttcc ggaagtgatc
     6541 ggccccattt tttccaccct tcgtcaacga gatgatattc ccatccgaat tcggcagcca
     6601 tatcgacaaa ggcatattgg tcgttttcca ggacattccc gcggtcccac caggtccagg
     6661 tggaacggcc cggcttgatc caaggggctt tggagccttc cggaaaaaga ttcctgtccg
     6721 gttccggagc cagggaaggg atgacgtcgt tccggaccag tccgttcagg tcgtcattca
     6781 ccaggatgac gcgccagggc atttccgagg atgagagggc ggaaaagccg ccgggatcct
     6841 ggaaatatac cgcacggcat gctccatcct ggagggaaaa tttaatgccg ctccatttcc
     6901 tgttgaagtt ggctgcttcc tgtatgagcg cgacgcctcc gtccggcagt tccgccgtaa
     6961 tcggcatggt gcggacacag ctgcggggat tgccgggatc ttttttgaaa tccgttattc
     7021 tggaaggaga ccagaccccc tcgcaggggc ccagggcgga ggaggcgtcc tgggtccaca
     7081 gaacggtttt ttccggaaag gcgaaggagg ttttttcttc actgatattc agcgagggcg
     7141 ctttcttcac ctcttccgtc cactcatagc ggaaggcgac cccgttgttg aagacacggg
     7201 cccggagccg gagggaggag ttcttcccgg aaaccacgta ttcattgaag cagacggaag
     7261 aggtggggta tttgccccgc gtctcgaagg tgtcgcgaac gttatccttc actttttcca
     7321 cattccatcc cgtgatgcct ccggcgaggt tttttccgtt cacgacgacg cccagcccgg
     7381 atggttccag aatcgttttg ccgccttttt tcagagaatg ggcggcctgg ctgcccgcgt
     7441 ggagcctcca ggacagccgc ccgtccggag attgcaggga tgcgtgcgtt tccgccgcct
     7501 gtccggcgcc ggtcaggagg catcctgccg tactgctgag cataatgagt gattttacaa
     7561 gcatatcttt atcagaatac gtccgtcttt gttcatttat tgcaaaacag cgaagcggaa
     7621 attggaacat ccatgttccg aaggagggaa aatgccgttc cattggaggc atccgggcaa
     7681 tttccggaat aaaaagggaa agcctttttt cttcccacat gaatttgtag cggtatgccg
     7741 ttttttcgtg aaggccgggg atcatttatg aagaaaaaat aatagatatc ttatgttcct
     7801 gtgcagatag ttgcaggaaa ataccggatt ggcagggagc aaaagaataa tctttacaaa
     7861 ataataggaa gcaatagaat aaagcgcatt gcaaatataa ggatggagcg tagcggttta
     7921 aatagtgatc ctgttttttc ggaccaggat gagcattccg ataatgtttc tgtacgcagc
     7981 tcctccaaat tacttcctcc cccttcctgg aacgagtctt ccgtttattc caaggaactc
     8041 ccggcgggag caactttgga acattataaa atcctgaagg tgctgggcag cggagggttt
     8101 ggcattacct acctggtgcg ggacatcatg ctgaagagaa aagtagttct taaagaaaac
     8161 tttccttctt cttatgctta ccgggatccg tttacagggc gcgtgatacc caacaatgaa
     8221 cacgatgccg agtctttcca gtggaccctt tccaactttt taaatgaggc ccgtgttctt
     8281 gcacaactgg attcgcccgg tatcgtgcgg gttctttccg tgtttgagtg taacggaacg
     8341 gcttattttt ccatggatta cgttcagggt cttcccatgg attatctggg agagcagcag
     8401 cttcttgcgg ggaactgtta ttccgaggcc aagttgaagg ggctgctggt gcacatcctc
     8461 cagattttgg attaccttca taaaaaaggc atctgccaca gggatatcaa accgggaaat
     8521 atcctgctta cacaggaagg ggctcccgtc ctcattgatt tcggcgcgtc gcgccgtatc
     8581 gagagcaacc acacccagac cgttctggcg acacgtggtt tttcttctcc ggagcaggcg
     8641 ctgggcagaa aagatatggg gccctggagc gacctttatt ccctgggtgc tacattttat
     8701 tccctgctga agggctttgc gccgcccaga ggggaggaac ggctgatcaa cgacaccata
     8761 cagccgctgg ccgcatcctc ttcgttgtct tcccattatt cgaaaaattt tctgaaaacc
     8821 atcgacaagg ccctttctcc gcgtgtggaa gaccgttatg cttccgcaga agcgtggatg
     8881 aaggatctga gggttgaggg aggcgccgtt tcaaccattc agttctcccc cgaggagttc
     8941 aaatcagccc gtaagcgttt tttcaatttg tttgccttat cctcttcccg cattatgtcg
     9001 gaagggcagg aacatttgag gacgagtcgg aagatagcgt ttgggatgat tattgtcctg
     9061 ggaattatag gcgcgcttct gggatattct attcacgggg ggaacagaac ggagaatgaa
     9121 gatgcgttag ggctgcgttt tcccagtgtg atgtccactc ccattcccca gattatttca
     9181 gattcaaggg atgtgtcatt gttaagttcc atggatcatt ttgacgtcag gctggatagc
     9241 ccgtctttat cgcgtcatgc tctgccgtcc gtgctgcccg accgtttttc acttgcatgc
     9301 gttcatttaa gagtgcttca ggaagacaag atgtgcccgc ccctgtatct gaccatacgt
     9361 gatgagcagg ggaagctggt ttcccggtca tttaacggtt tttccttcac cttgattgaa
     9421 agtccggtgc ttacaggatt ccgttttgtg gatcttcctg ctctgaaagt agatgcccct
     9481 tacagattca gttttgagac caaggataat gttccccatc ctttgaaggt ggtttcttcc
     9541 cagattacga cgctggagag cgggaaagtc ctgaagcccc atttcaaatt catctgcgcg
     9601 gcgtatgatc ccggtgtgaa agcttctctg gacagcccgg agaacagcaa actccgggaa
     9661 ctgcttatcg cttcccggaa aaggaaccgt acggaaattg attccatcgt gctgacggag
     9721 cgggagaagg ttcagttgcg gaaattcgcc agggcggggt atccttccgc gcagtataaa
     9781 atagcccgtc ttattttcga acgggatgga agatacgggg aagaaggtgg cgcctggctt
     9841 tataaggcgg ccatgggcgg ctttattgtc gcccagaggg atttggcgtt tcttcttgta
     9901 ggcggcaagg agtttttccc ggatattctt cagtgtccgg tcagcctgtc ccagaactat
     9961 tcccaggcgg ttgatttgct gttgatgtct tttacggcgc aggatccttc cgccctgtac
    10021 atgctgggca ttttgtattc ccaggggtgg ggggtggatt attccgcaga gatggtcatg
    10081 aggctgtcca aactgttggc cggttccggt tatgatttgt cgcggctcac tttgtccgcc
    10141 cccattttag cccattggga gcttctgggg cagcaggatg gcgcttatac gcggatgtgc
    10201 tgttccattc cggacaggaa gatgaagcaa ttttcaggtg ttcgtctggt gcggacttcc
    10261 aaagaggggg gaagtctttt tattgacaag gtaattctgt tcagggacgg gcaggaagtt
    10321 atgtcccgtt ctttccgttc ggctcttacc caggaatctc ccgttctgga ggtgtccatg
    10381 aatatggagg attacgcaga gaatgtggag aatttcgagt ggtcaataga agtgatcatg
    10441 gctccggcca acacatgcgg catcatggaa atgatggaaa aaaaggagct ttccaacgat
    10501 aaagagaagc ggatttgatg gaaagctgcc ggagcggtct ggcggatacg cggtttcgta
    10561 ttcctgccgc aaccccgttc ccggcaatgg aaaggggcaa gaactgtttt cccatgggtg
    10621 aattcagcca cgggatgacc gcagcggatt taaatgtttt tcagcatgtc cagcgtttcc
    10681 cggagtttgt ccgcgggagc ggcgcttgcc agccgcggga atttgttgga caacaggata
    10741 aaatccccgt ttctggtgag catcaggcgc tgtccgctga tttccaccat ggagatgttg
    10801 gcatgggatg ccaggatttt gattttgtgg gagtccagca ggtggcgcac cgtttccggc
    10861 gcggggccga agcggtcttc ccaggcgcgg agcagattgt ccgcttcctt aagggttcgg
    10921 acggcggcca gatccttgta ggcggagatg cgcagggccg cgtcactgat gtattccctg
    10981 ggaaggaagg ctcccagata gtccttgcgg gattttgcgg cgaactgcgt ttcgctgttg
    11041 atgatgaagt ccgttcttag agccgcatcc ggacgggggg ccgtgcgttt gccctgcatc
    11101 tgggcgatgg attgctggag aagctggcaa tacaggtcaa agcctatggc ggcgatgtgg
    11161 ccgctctgct gggttcccaa caggttgccc gcaccgcgta tttccaggtc cctcatggca
    11221 attttgaagc cggaacccag ttccgtatac tgtttgatgg cggagacgcg tttgcgggcg
    11281 tctcccgtgg tggtggcgga tctggggagc atgaggtagg cgtaggcccg gtggcccgca
    11341 cggcccacgc ggccgcggag ctggtaaagg tccgccaggc cgaagcggtc cgcgcggtca
    11401 atgatgatgg tgttggcgtt cggaatgtcg atgccggatt cgatgatggt ggtggccagc
    11461 aggatgtccg cttttccctg cacgaaggtg cgcatgacct tttccagttc ttctttaggc
    11521 atttggccgt ggccgatgac gatgcgggcc ttgggcacca gggcctggat gcggtcccgg
    11581 aacagttcga tggttttcac gcggttgtgc agcaggaaaa tctgtcctcc gcgctccagc
    11641 tcgcgttcca tggccttttt catgatgcgt tcgtcgtaag ggcacacgct tgtctggacg
    11701 ggctgccggt tgacgggagg ggtttcaatg gagctcatgt cacgtgctcc catcagggca
    11761 atgtacaggg tgcggggaat gggcgtggcg gacagcgtca gcacgtcaat tcccttgaag
    11821 cgttccttga atttttcctt gtgtctgacg ccgaagcgct gttcctcgtc aatgactacc
    11881 aggcccagat ttttcatgga tacgtcatcg gaaatgagcc ggtgcgtgcc tatgacgatg
    11941 tccacggctc ccgttctgag cccttccagc gtggagcgga tgtccgccgc gctgctgaag
    12001 cggctgagca tttcaatacg tacggggtat tcgctcatgc gttccttgaa cgtgcggaaa
    12061 tgctgttccg ccaatacggt ggtgggaacc agcacggccg cctggcggcc tcccgtcacg
    12121 catttgaagg cggcgcggat ggcaacttcc gtttttccga agcccacgtc tccgcagatc
    12181 aggcggtcca tggggcgggt ggattccatg tccgctttcg tctgggcgat ggcgcggagc
    12241 tggtcctggg tttcccggaa gggaaaggag ctttcaaatt cccacatcca tttgctgtcc
    12301 ggggggtggc tgtagccttt tcctgtctgg cgttccgcct ggacattgag gagctgggcg
    12361 gcgtaatccg ccacggatcg ttctgcggat tttttggcgc gctgccattt ggagtccccc
    12421 agtttgttga gttccggcgt tttgcttccc aagcctatgt atcgtgagac caggtgagcc
    12481 tggctgaggg ggacgtgcag gaccgtgttg tcccggtaga ggatgttcat ttcctcgtct
    12541 ccggagtcag gggaggcgga aatgttgatg aatttgccga tgccgtagct ggtatgcacc
    12601 accagatcac cgggattgat gtctttcagg gatgcctgcg cgcgtgcctt gcgcgccttg
    12661 tcttcccggc tggcgcggcg gcgcgccgta gcggactggt acctgccgaa cagttccgag
    12721 gaagagagga cggccgtttt cgcttccggg atgctgaacc ctgccggaag ttctccacga
    12781 agaggggtga tggacagcag agcgggtgtt ccggcgcaga tgtcccggaa acgttcttcc
    12841 tccccttcat tggggaagaa catgctgaca ttccatttcc ggtccagcca ttcccggatt
    12901 tggcgttccg ccagttcacg gcgcatttcc tgcatcacga agtcccccgc ctcaaagctg
    12961 cccaagggat tgtcatggat ggcaagggag aagttttctt cccccgccgc attttccggg
    13021 ggggaggtga ggatgcatac gttcccccgt tccttgcaga aaggggcggt aataaccagg
    13081 tcgcccggtt tgatccagtc ccgcagggtt tccgcaccct cggcttccgt cagtatgagg
    13141 ttgacgtgtt ccgccttgcg gaaggaaatc tgggaatcca catcaaattc ccggatggat
    13201 tcaatttcat cgtcaaaaaa ctccaggcgc acggggtggg aggactggag aggaaagaca
    13261 tccaggatgc cgccgcgtcg ggaccattgc ccgcgggaaa tgacctgtgg aacaccttca
    13321 aatcccgctt ccccaaactg ccgtatcaag tcgtccggtg cgtgcgtctc ccccgttttc
    13381 agggtgatcc cctggttttg catgccgtcg ggcgacggaa cctcatcgtt caggctgcct
    13441 tccgttaaaa tgatggtttg cccgcccgtg cggctggaag agatgcggtg aaggatattc
    13501 agacgttccg cggcgagctc cgggtccccc atttcctccc ccaaggccac ctctttttcc
    13561 ggaatgaaga ggaggtggcg cactccccag gtttccagtt gggctgcaag ggcttcctga
    13621 gcccgcaaat ggggcgcgac cacccaggcc cgggcggtat ccggaagggt tctgatgcac
    13681 agggccgcta caaacggcac cccgggcagg gaagtatggt cgaaaaccgc ctgttcctcc
    13741 gtgcccagtt tcgccagttc ccggtgaaag ggggcggta
//
LOCUS       DAWEMX010000060        13699 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1863_length_13699_cov_13.079156, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000060 DAWEMX010000000
VERSION     DAWEMX010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13699)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1863_length_13699_cov_13.079156"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12329..12338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2738 a         3411 c         4175 g         3365 t
ORIGIN      
        1 tgggggaggc gcggttgatt tcatccgcca gcataatgtt ggcgaagacg ggcccttcct
       61 ggaagatgaa gttttttgtt ccgtcccttt cctgaagaac cggggttccc gtgatgtctc
      121 ccggcagtag atcgggtgtg aactggacgc ggcgcgctgt gatatccagt gccctggaga
      181 ggccttagac cagttccgtc ttgcccaggc cgggcaggcc ctccagcagg atatgcccgc
      241 gagccagtac gccaatgaga accagttcaa tgagatcctt ttgaccgaac aggacctggt
      301 tcatggcggc agccagagca cggagctttt gcatggcgca gccaagttct tcctgagaga
      361 ggataggggg ggtattcatg ggaaactgac ggatttccgc ggcttttggg aaaaagaccg
      421 ggcatttgtt agatttcttc atgaacttat gccatgcatc cagaggggct gcaagcagaa
      481 agatagcggg acctgttaat tttcagcaat atgaagagaa aataccctgc ggggtttgag
      541 tggtaaatgg aattgccgca atatatgttc catctgttga aaaaagaagg cttcctggca
      601 tatattattg tcatgccttc cgtatatccc cccggcaacg aacgcattta cgtggtggac
      661 gctctgcgcg gctttgccgt aatggcgatc atgctgctgc attttcttga acatttcatc
      721 tataattcct atcccgtggc ttcttcccca atgatggaag cggccaacca gcagttcaag
      781 gaggtctttt ttttcctgtt tgccggcaag tcctacacca ttttcgccct tctgttcgga
      841 ttcacttttg cggtccagta ccgtaaccag gcccggaagg gaagggattt tgcgggcagg
      901 tttgtctggc gcatgtgcct gctggcggtg ttcgcctgca tgaatgcagc gtttttcccg
      961 ggcggtgatg ttctgctgac tttttcactt gcaggtttgt tgctggtgcc gtgccgccgg
     1021 ttgaagacat cctcgctggt ggctttgtcc ctgtttttcc tggcccagcc gctggagtgg
     1081 gcctatgcgg cggtgcagtg gatgcatccg ggctgggttc ctccctccct gtctgttgcg
     1141 gaatcgtacg cttccctgaa atccgccgtg gatacgggaa atttctgggt gatggcctgg
     1201 gagaacctga ctacaggaca atgggccagc ctggcatggg gtattgaagc cgggcgtttg
     1261 atgcaggccc cgggtctgtt cctgctgggg tttgttctgg ggcgccagga tttctttctg
     1321 cagaatgacg gaacggctgt gttctggacc cgcggactgc tgttttccct ggccggggcc
     1381 tgtgcatttt acgtggtgat gtgcctgccg gggctgccgg ggcctgtgca taccgtgttt
     1441 agcatgtggc acaatgtttg ctttaccggc gtatgggtgg ccgggttcgt tctgttgtac
     1501 cggctggaat atttcaggaa agcgacggct ccgctgctga cctacgggcg catgagcctg
     1561 accaactatg tttcccagtc cgtgatcggc tccctgattt ttttcccgta tgcactgggg
     1621 ctggctccct actgcgggta tctggccagt tttgcggtcg gctttgccgc catgaccgga
     1681 cagatatggt tctgccgctg gtggctggag cggcaccgct acggggtgct ggaaggattg
     1741 tggcaccggg cgacatggct ggccgccggg aaggtttccg ctccggaaca ccggtaagaa
     1801 acgggggccg gcgcaatgat gtttcccgat acttgcggga cgctttttag cgtcttctgc
     1861 caaagccggt gatttatccg gacggaggat gcatccggtt ccgcaagggg cattttggag
     1921 aggagacatg gaacaggggg cagaggcaga gggaaaaggg atgaacgttt catggcggcg
     1981 ggagacatga aagcgcggta ttttttgaag gatggctttc cgggagattt tttccacagg
     2041 gaacggtctg ttttcctggg gatggcgtgc cgcgccctct ccggtccgtg ttccggcggc
     2101 ttttccgacg gactgatgca ggaggggatt ccgtaccaga tggcgctttt ctttaaaggg
     2161 gagatgtagt atggcatggt gtatggaaag gaggacgggc ataggcggtt ttttcgggct
     2221 ggaattgccg gagtacggca atttcccgca atggcatccc ggcaggagtg tagccgtgaa
     2281 ttccggcaga cgcgctttgg aatatatcct gcgccgcctg ggggatgtgc gctgtgtccg
     2341 tgttcccctg tatacatgcc ggacggtggt ggaaacgatg gaacggcttg gcattcccgt
     2401 gattacttac cgcattgacg aacgcctgga accggaggaa ctgccggaat tggaaaaggg
     2461 ggaatacctt ttgtacacca attacttcgg aatcaaggag aaatgcgtgg accgtctggc
     2521 ttcccgatat ggcggcagcc tgattgtgga caatgcgctg gccctgtatt ctccggcccg
     2581 ttccggagtg gcctccctgt acagcccgcg aaagttttcc ggtcttcctg acggggggat
     2641 cgccgtgatg gatgcggagc ggtccttgcc ggaaccggag gagcgggacg aatccctgcc
     2701 tgccgccgct tttttgctgg aatgcctgga gcatgggcag gaaagggctt ccgccgcctg
     2761 tgaacggaat gaacggcgct tgcggaatac cccgctgcga agggtttccc ggctgacgga
     2821 gcgactgatg agcggcattg attatgaaca ggctcggctg ctccgcatga atcatttcct
     2881 ttttcttcat gaaaggctgg gccatttgaa ccgtctttcc gtggaggcgg attccttgtc
     2941 cgctccgtgt tattatccgt tttggacagc ttttcccgag ctgaggaacg tgctgattga
     3001 taagcgcgtt ctgattcctc tcctgtggcc ggaggtattg gaacaggccc ctgccggcag
     3061 cctggaacgg aaactggcgc tgcatcttct gccgcttcct gtggaccagc gctgcgggcc
     3121 gcaggacatg gagcgcatcg cccggattgt ggaggcattc tattcctgaa gagaagggag
     3181 gattattttg ttagacagtt ggataagacg ttgacggtac gggaatgaat gttctgacat
     3241 tttgggtatg tccgactacc tttcctctct gggctatcct ttccgtggaa ggcccatggt
     3301 ttttgtcttt tccgtcatga tggtatgggg catgtttctg gcggcccggg atgttctcat
     3361 cggatggatt gccgcctttt gttttctgct gcctttcatg tgcgcctttt tcatgaaaat
     3421 ggtgaggcag tccgccatgg gagaagagga tatctgcccc tttcctgaac tggatgaatg
     3481 gcttgactcc ctcatgctgc cttttgtccg gctggcggtg tgcatcatcg tttccttcct
     3541 gcctttcgcc gtgtatgcgc atactgcggc ctgggcggcg gatattcccc tggcgtggct
     3601 gttcctggcg gcgggtgtga tttactttcc cggcgtattc atgagaacct ccgttcggga
     3661 acgtttttcc ggtctttctc ccgtgggctg gtgggggctt gtcagaaggg ctcccttttc
     3721 ctatgccggg ttgcttggcg ccatatgcgc cgggggatgg atcatcctga accttccttc
     3781 agggcctttg atggatttcg ccatggcgcc cgtgaagctg tataccgcct gcgtgttgat
     3841 gaatatgtgc ggactgttca tgctcaagca tgaatttgcc ggggaggaag aggacgactg
     3901 cttttcccct tccgggcctg cggcctcgtg atgctgccgc ctttccggcg gcgccgggaa
     3961 caggtgagtt tatttggcgg gttccttgtc caggttcagg atgcccagca cagacatgtt
     4021 gccttccgca tcttttccgg aggggagata gaccttcgca ttgttcagca tgttttcaaa
     4081 ggccttggcg cggagcatgt tcggtcccat ggccttttcc tttaatgcca gtccttccgc
     4141 ttccgcccgg gctgcttcca gggctgcttc cgcctgggcc ttgccccgca tgacaatgac
     4201 tttggcctcc gcttctcccc gggccaggtt agcgtttgct tcctggcgcg ctatttccat
     4261 tttcatggcg ccttccgcct ccccgcgtgc gcgctggatg gcgatgtctt tccgggcgat
     4321 ttgaagttcc gtttccttgc gttcgttttc ctgagtcgtc tgaactttcc tgacgacgga
     4381 ttcaatgatg gcctgcgggg gatttgtttc ccccacggct acggaatcca caataaaggg
     4441 ggtgccgtcc agccgcttgc ttaactgcgt gtaaacgttg tcggaaattt tttgcaggct
     4501 gccggaggcg tccagggcat tgtactggct taattccgcc cgcacggctg tgcggaaagg
     4561 ctgctgaatg aaatttttgt aggcaaagag catgatttcg tccgccactt cgtcatcatt
     4621 gcggccggag ctttgggcga tgcctccgta atcttccatg aattccttga cccgggacgg
     4681 gtccagccgg taaaccagag aagctttgca ggagatgggg agcttgtcct tgcccaggat
     4741 agcccctcca ttgcgggcgt cgaattcttc caccgtggta tccggagtga tggaaacctt
     4801 gctgacttcc tttctccacg cccatcccgt ggaggacggc ccgatcagga tgccttcaaa
     4861 cctgttctgt ccgaaaatgg gtttggaata gacatatccg gcaaagccgg acaatacgcg
     4921 cttgttactg aaattctgga aggcaaaaat ggcggcgccc gcagcgacga agatgacggc
     4981 gggaatgagc agggagaaga cggttttggg agatttattc atgacagggc agcggttgtg
     5041 acggtatcca acataagatg aggctggtga aaatcaagtt atcttttcct gtgcggccgg
     5101 gaaaagtgtt ccactgaaaa aattctttcc ggaagaggga atgcggcaaa gtatgtcctc
     5161 ttctgaacac aggggagtgc cggaaatgtc ttatccggca aaggctgcct gttccgtggg
     5221 ggagattccg gcgaggtgcc ggacctccgc cccggaggcg ggcctccatt tgggttaagg
     5281 tgatgaatgt agccattacg ggatccggag gatttatagg gagtcatttg tccaagcgtc
     5341 ttgagatgtt cgggcatcgg gtggcgccct tgcggcgcta tctgtttgcc caggagggcg
     5401 tggattgtct ggcgcaggcc ctggcgggat gccatgccgt catcaatctg gccggagcgc
     5461 ccatcgaccg gcgctggacg gatgcctaca agagggagct ggtggagagc cgcatcatga
     5521 ccacccgcaa gctggtggaa gcggtcaaca ggctgcatga accgccggga gtgatgattt
     5581 ccgcctctgc tgtcgggtac tatggctcgt ccggcggctg tcatggggaa ctggacgctc
     5641 cggaagcgga ttcctttctg gcggaattgt gcgtggcctg ggaaaaggag gccgggctgg
     5701 tgaacagctc cgtgcggctg gtccttaccc ggtttggcgt agtcctttct ccggatggag
     5761 gggcgctccc cgagatgatg cggccggccc ggttcggcgt tacggcaacc gcaggaaatc
     5821 cggatcatct tttttcctgg gtggctttgg aagatctggc ggatgccctg gtcttcatta
     5881 tgggcaggca gtatatttcc ggcccggtca atgtaacggc gccggagcgc accaccaacc
     5941 gggaattcta cagggcagcc gcggaacatt tccacacccg tctgtccatc cgtgttccgg
     6001 acgccgttct gcggttgctg atgggagaag cttcccaggt gattaccggc ggccagtgcg
     6061 ccgttccgga gacgctgatg agggaagggt ttacgttcca atatccggat atccgcactt
     6121 tttttaacaa agcattttct gtcaaatcat gagcatgata cagaccatcg ttacttccct
     6181 gtttcacggt cattccgtca aaaccgatcc catgccggat ttcaatctgg aacgttatct
     6241 gggggaatgg cacgaaatag cccgtctgga aaactggttc gagcgcgggc tcagcagggt
     6301 tcttgcccgg tatgaccgca gggaggacgg ttccatttcc gtggttaaca gcggttatga
     6361 cgttcgtacc ggggaacgga aggaggcgtg tgcacgggcg gtggcggggg atgcccccaa
     6421 tcatttgaaa gtgtactttg taccactggt atacggccgg tatgaagtgg ctttcctgga
     6481 tgaaaattac acccgcgccg tagtttccgg cggttccctg aattatctgt ggctgcttgc
     6541 ccgctcgccg cagttgaagg atgacgagct tcagccgatg cttcattgcg cggaaaaact
     6601 ggggtatgat acttcccggt tgatttacgc caatgtccag gcctctccgg acaaatgaca
     6661 tggggcgggc ttgaagaagc ggtgtgccgt gaatatggga tatttttgaa atggggaatg
     6721 tggaaaaggt ggctttcctc ctgccaggaa aaaatttttg ggcatgggcc gggcgttcct
     6781 ttccagtgtt ccgccgtgcg ggaatgtcat gcttttgaat tctccggtgt ggttcttctt
     6841 cctgcatggc tccggtcccg tatttttccg ctttttcctg ccccgtcctt tccgtttttt
     6901 tgcaaagaac agggaaaaac acaggcgcct gcttaaaata ggcttgcaat catgagaagg
     6961 atggatatgt tggcattcat ccaaacattg gatgtttgga tgaattactt aaagaaggag
     7021 taacaatgga tattattcat gacaacgccg ctgatctcag cgcattgaac ggcaagaccg
     7081 ttgccgtgat cggatatggc gcccaggggc gcgctcaggc actttgcatg cgtgattccg
     7141 gtgtgaacgt gattatcggc gttcgccccg gcaagtcttt tgacgccgcc gcccaggatg
     7201 gattccaggt aatgagcgtc gcggaagccg ccgaaaaagc ggacatcatt cacattctgc
     7261 tgccggatga aagccacggc gccgtgtatg aagctgaaat caagcctcac ttgaaggccg
     7321 gcaagacgct ttgctgctcc catggcttcg cgtatgtttt caataccatc gtcccccccg
     7381 cggatgtaga tgtcatcatg gtggccccca agggcccggg cacggaagtg cgccgagtat
     7441 ttgaagaagg gttcggctgc cccggcctga ttgccgttca tcagaacccc tccggcaagg
     7501 cccgtgacgt ggctcttgcc atggccaagg cggaaggcct gacccgcggc ggcgtgctgg
     7561 aatgcaccat ggcccaggaa acttatgaag acctgttcgg tgaacagaac gtcctgtgcg
     7621 gcggtctggt ggacctgatg aagtacggct ttgaaaccct gacggaagcc ggctatccgc
     7681 cggaaatggc ctactttgaa tgcgtgcatg aagccaagct catcgtggac ctgatttata
     7741 acggcggcat ccagaagatg aactccgtga tttccaacac ggccgaattc ggcgaatatt
     7801 acaacggccc gcagatcctg cccgcagagg tgaaggaacg catgaaggaa tccctcaagc
     7861 gcattgaatc cggcaagttc gccaaggact ggctggaaga ggcagccaag ggcgctccca
     7921 acctcaaagc caagcgcgaa gccctgggcc agcacccggt ggaaatcgtg ggcgccaaga
     7981 ttcgcagcct gtttgaaagg aactaaggtt tcttttactt gaaacacgtg ccgttccgtc
     8041 ggaatacggc ggagcggcac gtatattccc gggcttttcc cggaaatccg ccattgattc
     8101 agtttatgga gcatgcctca ccggccgtca atgtagaaaa cgccagggta tacctgggcg
     8161 ggcacgaaat cctgcacaat atttcctggc aggtgcagcg cggagaacgt tgttttattc
     8221 tgggggccaa cggcgccgga aagacaacgc tggtcaaagt gctgatgggg tttgcatggc
     8281 cgcttttcgg agccagggtg caggttctgg gcaagacctt cgggcacgtg aacctgctgg
     8341 aattgcgcaa gtccattgcg tgggtgagtc cgttcatgca caagtggctg gaggatggct
     8401 cctggaacgg gcgcgacatg gtgctttccg gccccgacgg cactatcggc ctcctgaggg
     8461 agccgacgcc ggaagaggag gaaaaagccg caggaatcat gaaatccctg aaagcggagc
     8521 atttgatgga caggccggtg gtggccatgt cttccgggga acaggtgaaa gtgctgattg
     8581 cccgggctct gatgacgaat ccggaactga tgattctgga cgaacccagc gtttacctgg
     8641 atttggcagg gcgggaattt ttattgaaaa ccattgaaga actggctgaa acgaggcctg
     8701 agctgaccat tgtgttcatc acccagcgca ttgaagatat ccttcccgta tttaaccgcg
     8761 gcatgatttt aaagtccgga gaaatcgtgg cgcatggaag ccgggacgag gtgctgacgg
     8821 aggaaaacct gaaagacgcc tttgggctgg atatccagtt aatcaagacg gagaaggggc
     8881 gtctctggac cgttatccga taatttttca tgaagcagtc caagacaatg gagctgaaga
     8941 aaccggtgcg ggtgtccctg gcccgccagg tgcttaccgc catggaatcc atgatacggt
     9001 ccggcaaatg gaaggttgga gaccgcattc ccgcggaagc ggaactggcc cgtgcctttt
     9061 ccgtcagcca caataccatc cgagaagccc ttcaatccct gattcacatg gggatgctgg
     9121 aagcgcgccc cggggacggc acgtacgtga tggcgtcaga ccgctttgcc gtcgcggtga
     9181 gcaaccgcct caaggaatcc gagctgcctc agattctgga ggcgcggctg gctttggaaa
     9241 aggaaattgc gcggctggcc gccgttaaaa ggacggatga agatttgaag gagctggaaa
     9301 atgccctgaa ggactgccac ggcagggtaa ggcagggcat tgaggatgac atgctgtttc
     9361 atgccgccgt ggcccgcgcc acgcataatc cggtgctctc ggaattgtat aacgtggtca
     9421 tccgccatgt gcaggagaat ctggaaaagc tgcttcagga gaagcaatac gactccggcg
     9481 ccatgaagct gcatgacgac cttctcgcag ccatcaggaa tcaggaggcg gatgaggcgg
     9541 agaacattat tgtaaagatt gtggaatttg acaccgtcag cataggcggg tcattcattt
     9601 cttgaattgc cggcaggaaa gatgccgttg cggtggcaga gttattcttt tttatttccc
     9661 ggtgcttttc ttgccagcgg gctttccggc aaataactgc gttctggaat tgcctgccaa
     9721 atgaaaagcg ggaatttagt ctttccgtac caaggcgccg gaggcaattc tcatgatttt
     9781 tatccttttc cggatatgga attttactac aattcgaata tggctattca cactgaatgg
     9841 aagtgcccgg catgcggtgc ccttctggct atggaagatg tgaatatggc tgcggatatg
     9901 gtgttatgcc gctcctgcaa taagataagc aaattttcag agattgttca ggaggaaagg
     9961 gatgaggaaa ttcttgattc cattccccgc cgcatgagcg tgacgaaaac ggcgaggggg
    10021 ctggaaataa cgtacaagaa gccaaaaggt gcgggacttt ttctactgat gttttcaatt
    10081 ttttggaatt ctgtgatatg cgttgccctg tttgaggtga tgagggaaat gtcttgcgaa
    10141 aatctgttcg gactattgtt tttgatccct tttgttctgg ttggggctgg tacatttgcg
    10201 ggagcttttt atgtgttgtt tggccgtatg acgctgatcc tgacgccagg caggggggaa
    10261 ttgttccgag gagtggggag cctgggccgc cgacagcagt ttttgctgga gaaggattcc
    10321 tgtatttcca ttgaggcaag cagtattcga cggaatcatg aagttttaaa caagattgta
    10381 gtgggggtgc cggacggcaa gacgtttgag tttggtacag ggattgagga agtggaggcc
    10441 cagcagtatg tagcggctct gctgcggaaa atgaggggat agggctgtga tgcctgaata
    10501 cggagaaggt cttggaatgc cctggctccg ctgtttttca gcaggtaggg gaatccgtcc
    10561 tggcgggaag ggagcgggga tatggatttc catttccggg aaggaatggg cgcaagcctt
    10621 ttttgttggc aaaatccatg gatagcgggc agggggcgtt tgaggaatga tagcgggaat
    10681 tccgggaatg agtttttttg attgatatgg agaataaggt acgtagatta acgtatgatg
    10741 aaggaagtgc gccttttgtt gattctgtgc ggtttgtttt gcggaacggc agttgcgcag
    10801 ccccgccttt ccacaccgcc gaacatgatt gtcattctgg cggatgacct gggttatggg
    10861 gatttgggct gtacaggttc caagcagatt aaaacgcctt cccttgaccg gctggccagg
    10921 gagggggtgt tctgttcccg ggcgtatgtg acggctccga tgtgttctcc ctcccgcatg
    10981 ggactgctga cggggcgttt ccccaagcgt tacggcatca cgacgaatcc caatatccag
    11041 atggattatc ttccggagtc ccactacggg ctgccgcaga cggagaaatt gattccggag
    11101 tatttggctc cctgcggata ccggagcgcg gtattcggca agtggcatct tggccacacg
    11161 aagggatata cgcctccaga gcgcggtttt acgcattggt gggggttcct gggcggttcc
    11221 cggcattatt ttcccgtgaa gaaagaggct gaaggcctga atccctccat gattgtatcc
    11281 aattttacgg ataagacggg catcacctat ttgacggacg acattacaga ccgggcggtt
    11341 gaattcttgc aggaagccgg aaaggataag aaaccgtttt tcatgttcgt ttcctacaac
    11401 gccccccatt ggcccaatga agccaaaccg gaggatatcg ccaaattcag gaacgtgcag
    11461 aacggggaac gacgggttta ttgcgccatg gtatatgcga tggacagggg aataggacgc
    11521 attctggatg ccttgaaagc ggacggtttg gaaaaggata ccatcgtcgt cttcctgtcg
    11581 gacaatggag gcgcgccgga agcttcttcc tgcaatgccc ctttccgggg ggccaagagg
    11641 cagcattttg agggaggtgt tcgcgtacct tttattatca ggtatccggc ggacaagcgt
    11701 ctggttcccg gaagcgtttg cagacagccc gtttcctccg tggatttgct tcccgctttg
    11761 ctgaaggcga atggccgtca cattcccagg aagttggacg gcatggatat tctggagctg
    11821 gtggggaaca agggagctcc cgttccgcgc acttttttct ggtgcacgga ttacacgtcc
    11881 gctgtgctgg ccggtgatat gaagtacctg ctggtaccgg atcgcgctcc gcagttttac
    11941 aatgtggcgg atgatcccca ggaacagagg gatttgtatt tttccagaca ccagaatgcg
    12001 gatcccctcg ctaaaaagct gggaacatac ctgactacga cgcctgcatg ccgttttccc
    12061 gacagcatca gctggtccgc caaattgatg agggagtatg acaagactgc gccggacagg
    12121 cagcctgagt aaggttgcgt tggctttgtt ccgtattccg tcccgtatgt cggcgtttgg
    12181 gaacctccca cggcgggaga gggggaggaa ccgcgtgctt ggtttccttt ccccgctgaa
    12241 aaatgcttta tttcaggcgc ttgggttccg caatgcggag aatgtgctct ccgggaaggg
    12301 ccaggttgcc tttgtcccgg gcaatcatnn nnnnnnnntt tcaaagtagt ggggatcctg
    12361 tgagatccag atgaattcgt tttttgcctg ttccatctgc tccctggatt tttccaaatg
    12421 agcctgaagg aaggaacgtt catgctccag ggagcgcaga tccttccatg gcttgaaaca
    12481 caccagggaa agcagcatcg tcaggcacag ggccaagaca atggcaaaga ggcgcagggc
    12541 tctctggacg atcagggcac gtttctcccg gcggatttca agctccgcca gggtaaaacg
    12601 gtggtagtag cggcgtctcc agagcattcc ttgcagtcaa acgacacatt tttgatcata
    12661 gaatatgaat tttaccacca taaactgcgt catttcccag ttcttcttca attctaagca
    12721 attgattgta tttggccatg cggtcggaac ggcttagaga gccggttttg atttgccccg
    12781 cgttcgtcgc tacggcaata tccgcgatcg tggcgtcttc cgtttccccg gagcggtggg
    12841 agatgatggc ggagtatttg ttatccttgg ccagttccac cgtatccagc gtttccgtca
    12901 gggaacctat ctggttgact ttcaccagga cggcattcgc tacatggcgg gaaatgccct
    12961 gattcaggaa ttccacattc gtcacgaaca ggtcgtcccc taccagctgg gtattgccgc
    13021 ccatggcttt agtgagctgt tcccagccca gccagtcgtt ttccgcacag ccgtcctcaa
    13081 tggaaatgat gggatatttt ttctggagtt cctgatagta agccgtcagt tcctccgctg
    13141 ttctgcccag gccgtctgat tttttgaaga cgtacaggtt ccgggaggga tcgtagaatt
    13201 cggaggaagc cacgtccagg gcaatgaaga tatccgtgcc cagggtgtac cccgcccgtt
    13261 ttacggcctg tgcaatgcat tccagggcgt catctgctga tttcagggcc ggggcgaatc
    13321 ccccttcatc ccccacggca gtggaaaggc cgcggtcatg cagcacatcc ttgagcgcat
    13381 ggaaaacttc cgccccgtag cgcagggatt cccggaaagt gggagccccc ttgggcatga
    13441 tcatgaattc ctgaaagtcg atgggggagt ccgaatgggc tcctccattg atgatgttca
    13501 tcatggggac gggcaaaacc ttggcgttcg gtccacccag gtatttgaaa aggggaaggt
    13561 tgagctgaat ggcggcggct ttcgccacgg ccagggagac gcccaggatg gcgttggccc
    13621 cccggcggga tttatggggc gtgccgtcca ggtcgatcat gattttgtca agggccggct
    13681 gcaggttggc atcgtgccc
//
LOCUS       DAWEMX010000061        13497 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1912_length_13497_cov_9.884690, whole genome shotgun sequence.
ACCESSION   DAWEMX010000061 DAWEMX010000000
VERSION     DAWEMX010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1912_length_13497_cov_9.884690"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3688..3697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8911..8920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3466 a         3773 c         3052 g         3186 t
ORIGIN      
        1 gagaaatcta tcataccaca cctttttgtc aatttttttc gtgctgtttt ttgcgatttg
       61 ttgagatcaa aatcaaaaca tatctgatta tcaatgtatt acatttttat ttttttctaa
      121 taaaaaagcc tctttcacca cgaaagaggc ctaaacgttt cagaagtcct aagtttctct
      181 ctactttttg cctcctccac tctttttctt catgactgcg tattgatgaa ccagaggcag
      241 ttcccggagg gcgtttacag catccttcct tgcctgggca taatcttttt tctccattgc
      301 catattcatc atcacttcat agcaggcggc attgtatccg ccatcatccc tcgtattaga
      361 atccctcata atctggagag cgcgccgagc cgccttatag ctgtatttac gcatttcctc
      421 cgagccgtta aggattttca ggatatagga agcaagcgaa tcttcccggg ggaaacgctc
      481 caagacgtgg tcaaagatat caaccatttt ccgataattt ttctccttcc aagccttttc
      541 cgcccgcact tccgccacag tagacgcaaa ccatccgtta tcggaaaact gccctgcatt
      601 ttcctcaatc aacttctggt attcatcaaa tttcttttcc cgcctcaatt caaaggattt
      661 ttttatgaca gcatacatgg cctgctggcg ggccttccgt ttcgcatctt cttccgcaaa
      721 cctgtttttg tcaaaagagt ccaatctggc agtttccgcc acccattcgg gcatcttctt
      781 aatttctcca gcccacagca atgttccgtc acgaacgacg accgcggcgg gaaatgagtc
      841 aagtttgagc aaatcacaaa attcaatgac cgaacgatcc gatggaatac cgacaggata
      901 aggagtccat ccttccggcc cggataaata gtcccgcatc tgtttttccg ccgacggatt
      961 gagccccaat accacggctt ttactcccgg atggtctaag agcccgtgtt ttttaagaac
     1021 ggcatcaaga gctcttccag gagctgtttc tccaagtata ttattgtggc ctctccagaa
     1081 gctgatgaat acggttttgc cgacaggaag tcctgttaga ccgggatgat tgaaccatct
     1141 taatttgctg aacaggggag cgtactgttt gtcttcctgc actactccca tttcctgcat
     1201 cttcctcgga ggttccggtt ttatttcagc aaccgcctga tttacaatat tctgaaagga
     1261 ttccttatta cctgaaagct gctgcaactt cgtccaggtt ttgctgatgg acgttaagga
     1321 aagcgccttt tccatatcct gcaacgcttc ttccatgttt ccctgccgtt ccctaagttc
     1381 cgccatcagt ttccatggca aggaagcttc cttgttattc cttctggaga tttcaataca
     1441 acggttcaga catctttcca ccagggcggc atccattttt ccggcaggca cggattttcc
     1501 atttagaagc gttcccgcca catcaatctg tacacggtaa ttgtccggat actctttgca
     1561 caggtcatcc agtacggctt gagcttcctg gaatttttcc aattccgcca agactctgaa
     1621 tggaaccatt ttcagttgaa tgatctgatc ctgctgaaac tgtcccgaat cctgaatttg
     1681 ccttaaaaag gcctgagctt ccccctttcc tcccttccgg gcaagctctc ccgttctctc
     1741 agaaacctgg cggtagagct tccatgcacg ggcgtttgcg ccgggattat cccccggaag
     1801 agatgctgca gaaaaatcag gcttcaacat ggcccgcatc atttcttcag aaagttttct
     1861 aggatgccct ctccaaataa gcttgccgtt tttaacggca aaagcatggg ggatgccgtt
     1921 aacccccatt ggagacagcc atttatccct ggtttttttc ccatccacat ccacggccat
     1981 ggcataagta aggggcgatg gacggtttct taaaaattct ttcagagatt ccggagattt
     2041 cctgtccatg acgttaactc cgataacctg catatgagga ttattcttaa atgcctgatg
     2101 caattgttcc atatggggca tagcagccag gcacgggcca caccaggtag cccaaaattc
     2161 aaaaatgtac actttgtcct tttcccattc ctttaccgga gttccctgta tccatacatc
     2221 cggcaaacca tccagagcaa gtgatggaag ggcatcctcc gaagcaaccg ttttgttttc
     2281 gtttttctca gaagaagctg cgtgcaatgg cattccggca gacaccgcaa agatcatgga
     2341 acaccctaaa accaacagag aggaatttaa catggtaaca cggtactatc gtaaccttct
     2401 gcgtcaagcc aatagaaaaa gaaaaggaaa gatagaagag ccgttatcgt gttctttcca
     2461 ccagaataga atacaaaata atcccaaaaa gaaaagatat taatcatgga gtttttcata
     2521 tggaataaga aaacaaacac gctcagcatc cacacttttt tgagtttcat tggatttagg
     2581 aaaaatgatt tacctttttg gatacacaaa gacaatatta ttttctaaaa tagaaaatat
     2641 accagaaatg gcttgccaga tttttctgaa tgcgaaaaat gcgctcatgg atgaaatgaa
     2701 gtccagtatc agaaagtttc tcgctcttac gaaaatgacc cgggacgaat ttgccgacct
     2761 ttgcggcgtc agcaaaagtc aggtcgataa atggctttcc actgttccaa ttccccgtgc
     2821 cagacaacgc cttatcatca gaatcatgaa ggaagagtac gccaaacagg cacgcctggc
     2881 tcaaacaaaa aatccgaaca gcatccacgt tcccgttact cctcaaaagt atgaaaagtt
     2941 caggaatgaa gctgaacgcc atggcctgac cgttccggaa tgggcgagtg aagcactgga
     3001 tgctctttcc agcatcaaaa gcagaagctg aaaagaggat ccgatattcc ggatcgattc
     3061 cagagaaaga gaaaatagtg ttgttatcgc ctcttcccta tcttttcttt cattacaaat
     3121 ctgcacaaat tcatgtctga cgacgatata cgcattttgg tgaaggagtg gctacaaagc
     3181 caaaaccgca aatcctatca attggcggag gagataggca tccggccgca aactttttac
     3241 gcacagatga gcgttcgtaa aatatcggcc aataccaaaa aagcgctttc ccgcatcatt
     3301 ccaggcctgg tttccgattc agatctgaat ccaccttcca atacggaaga atgtgacccg
     3361 aaaaaactca gaatcaagga atggctccgg gctcacggca aaacacgccg atggcttgcg
     3421 gaacaatgcc gcgtttccgt ccgcaccgta catacctggt ttactgcacg cggtaacatt
     3481 cctgtcaccc aatatctctt tatcgataag ctgatgagtg aaggcgagtc gtggaaagtt
     3541 cagcttccca ataccattcc cgtgaatttt ccggaaacgg aaatggaaat tgttcacaaa
     3601 ttccagaaac tacatccgga catagatctt cccatgtacg gcatgcataa aatcctggag
     3661 ttatgcgtta atgtactcac tcaggatnnn nnnnnnnatg aatattcaaa gacgtctcca
     3721 tactcccgta ttaccaatac cggagagatt cgccaccgcc ggataaaata agaattattc
     3781 aaaagttcaa attttctttt ccacgggagc atttctgaaa atgcactgtt tgcataccca
     3841 tgccggtatt tttctaaaac atcgcaggtt ctccgccagc ctggccttcc gggccattct
     3901 tcttaatctt tcacggaacc ctttcatttt tttgttcctt ttatcacgac ggccacagac
     3961 tatcctatct ctcaacacag agaaaaaatc ttcagccaca ttacatcatc cctccagttt
     4021 caaaaatcaa tcactattga ttggaaaaat agtccgaacc atgcgtaata gtgcttctat
     4081 gcagcctgtt caccagagtt aaccatactt accttgcctc tccattttta agaaagccgg
     4141 cttttggtga aaaagctctt atcacatgaa aaaccccgta ttcttaaaaa gaatacgggg
     4201 ttaaaaaatg gcacgtccaa ctggattcga accagtgacc cactgcttag aaggcagttg
     4261 ctctatccaa ctgagctatg gacgcatgat gcgacaaaag agtaacagag ttcatctcaa
     4321 cttggcaagc ccggatcttt gaaaagcgcg ctttaagcgc cggagcagcg gtcatgcgta
     4381 aaaggtatgt ccaccagggt cgctccaaac ttccgcacca gaggctcctg acaggaaaac
     4441 atgcgttccc gttcctccgg ttccgtatct atatatccgt gcagatgcag aagaccatgc
     4501 accatgtacc ggaaaagttc tctgaaaaca ggttctccga attccgaagc ctgccggata
     4561 gcggtatcaa agctgacgat gatctctcct attccgtctc catgcggaaa agtaatgaca
     4621 tccgtcgccg tcggatcatt caaaaaacgg gcatgcacat cccggatgac gtcatcggaa
     4681 accacgctga tttctacttc atccaattcg gaaagtacgg ggacaggacc ttcaggcaac
     4741 gccaacacgg acggaagaca actctccaag gctccaatca gggcatccgt aacggatgaa
     4801 ctgagcctcc cctcccccaa atgattgtag agttcaagat tcggtttcat tttcctccgc
     4861 atccgctccg ttcttcatct ccgtttccgt ggattttccc tcttgaaggg ttccggcatc
     4921 gggcaactcc atgatttcag gaacggcttt gccttcacct tcctttttca ccgctttctc
     4981 ttctttttcc ctgacggaca catcagagaa agcatttttc tcaatttttg cctccggatt
     5041 gtgagaagga taggcaatgc ggttgtggtg gatgcttttc agcgtcttaa tgaaactgtt
     5101 gcgaatcttt ttgagatctc cgaaggtaag gccgcaatcg tccaaatggc cgtccaccac
     5161 ccgttgcttc aaaagttcat ccaccctttt ctgcatttcc tcacaggaaa ttttacccat
     5221 ggagcgcgtg gcgctttcca cgatgtccgc caggctgacg attcccgttt ccttgctctg
     5281 cgggttgggc cccttgtaac ggaagttgga ttccaccacc tccgggacgt catccggaga
     5341 cgccaggccg ctttccacgc ggctcagaat ctcatcacgg tattgaaggg ctttccggta
     5401 aaagaagtaa gccagtgacg tgccgtgatg ctgctctata acgtccacca aaggacgcgg
     5461 aaggttgttc tccctggcca atgccacccc gtcctgaacg tggtcgatga tgatacgggc
     5521 gctcatgctg ggcgtcagct catcatgggg attggggccg tccatgatat tttcaatgaa
     5581 ataaaggggg ttttgcatct tgccaatgtc atggtagtag gcctccaccc ggcattcaat
     5641 gggattggcc ccaatggctt ccgccgcagc ttccgccagc tgggcaacca tcaggcaatg
     5701 gtgaaaggtt cccggagctt ccatttgcag ccttttcatg agagggcggt tcatatccgc
     5761 catttccagc caggaaatgg gcgtaatgat tttaaaagct ccttctatga taggcaggac
     5821 accgctgatc agcacgctgg tcagcatgct tacgccaaaa gccacggcca ggcaaaccag
     5881 aactccagta aggttgtagt cccatgcctg gagattgatg acacccatga tgcaacacag
     5941 caccatgaca agcagcccca caaagaagcc cgctcgcagc aactgagcac gattccgcag
     6001 gttatgggta agcaacacgg taagcatgcc ggagagagag ctcagaatcc agaattggac
     6061 ctgcttttcc ggagcatatt gctccggcag aatgaaaaat ccgccaagca tgcagatgga
     6121 aatagtggca aacatgccca gaagaggtcc cagcagcacc gtgacaacca acggagccat
     6181 catataggga aggtacaaaa gttcattgcc tatgtcggaa gtaatgtcag ccgttacatg
     6241 ccggatgaga ccaaaaaaca ggagctggac cagaaccgcc ccccacgtga cgacaaaggt
     6301 tttgtttttc ctgcgcaggg aaccggcgca taaccagtac ataggcatca gcgccagcgt
     6361 cacggaaaaa agcagaaaag cttccgacac atgatgccat atggattttc cccagtgggg
     6421 actgtaactg cccatccatt gaagcacgat gaaaaccagc acgcataccg ccaggctgga
     6481 atccaaacgg tcaccggctg aacgggacac ggaggcagcc ccttccgggg aggcactttc
     6541 ttttgccgct gcggcggcat gctttttcag aaaatgaaac atgataatta tttggggtac
     6601 acttcggcaa cctccggaat agtgcccagc cgggccatct cctcttctat gagggggttg
     6661 acgccctgct gcaccatttc catggaaact ctcagggcat tgcgaatggc tacaggggta
     6721 gaactgccat gcgcaatgat ggtcacgccc ctgacgccca gaagagggct tccccctacg
     6781 gagtccgcag aaaggcgcgc tttcatggca cggaatgctc cggaggcgca ggcagctcca
     6841 accatacgga acgggtttgc cttaagttcc tctttcagcc atttgctgaa agccttggcg
     6901 gtagcctcgc atgttttcaa caggacgttt ccggtaaaac cgtctgtcag agccacatct
     6961 atttcatgtt caaaaagatc gtggccttcc acatttccta cgaatttaaa agggagcgca
     7021 ccgcgctcct ccaaatgctt caacagacaa aaagttccct tggtaaaatc tgttcccttt
     7081 tcatcttcag agccattgct cataactccc accttaggca tctgtttgcc gtaaacagaa
     7141 cgcgccagaa tggccataac ggcataccct accaaatgcc ggggcttggc atccgggttg
     7201 gctccggtat cagtcacatt gcagactccg aattcattcg gaagctgggt cacaatgccc
     7261 gcacgttcca caccatccag caacctcagt ttgacggtgg cagccgctac agccgccccc
     7321 gtattgcccg cgctgaccac ggcatccgca tctccggact tgaccagatc cacggaaatg
     7381 gacatggatg aatttttttt ctttctcacc gccagcaatc cggattcatt catttccaca
     7441 acttccgcag ccggaacgat ctccacgcgc gggccggaaa gcccccagcg gtcacaagag
     7501 ctgcgcaccg tttcttccct tcccacaaga taaatttttt caatgagagg aaaatcctgc
     7561 agggcccgct tcgccccgtc cacgttaata tcgggcgcgt tgtcgccgcc catcacatcc
     7621 agtgcaatct tcatattatc tgttgaagaa atgtgttagc agaaaaaaca gtccgcttca
     7681 agcaagttca gccgctttgc gaagcaaaag cggctggaaa gtctatatga tagcgccgca
     7741 atacggcgat aattttcaag gcaaatgcgg cgcccgcgga acttacgctt ccgctttcac
     7801 cacaatttcc ttgccggaac gggtcgtgta cgtaccgcaa ttggggcagg cggtatggcc
     7861 gggggtgctg ctgccgcaat tctggcactt gcggagcttc ggcgcgcgcc atgcctgagc
     7921 ggcaaggcgg gtacgctgct tcatcttgga cgttctgcgc ttaggtgctg ccataacttt
     7981 agttggtaaa aaggtgatta atgatgatct cccagttcat tgagggcgtc ccacacgccg
     8041 ctattcctga tcggggcaga actgtttaca cccgttcccc cctctttgtc cactccaaaa
     8101 tacggagttt tcgccataca gtcgttttcc atgcctccgt cagcacactt cgggtatgcc
     8161 ggaaaatcca gaactatttc ttccctcaag gaatctgaaa catccacaac attctgtgaa
     8221 tcaatctgga aagaagtcgc gaaatcacaa acttccacca cgtagtcaaa gtagcccagg
     8281 caacgcacac agcggaattt aaagggggcg gaaatattgc ctcttacaaa caattcattt
     8341 tcaaatctct gcacatgcag atcaaaagca agaggcccaa tggaagtaac ctcttcatca
     8401 tcaatgccga aaatttcaga atccagttca ccgctgtatt ttttccctgc ctccggcaag
     8461 ttctccagtt ctaccagcaa tcttttcatg agtatatctt acaggcatac ttaatgcctg
     8521 acaagcaggc agggcggcac cattcatgga aaatgacaca tccgtccctc tttcaggcaa
     8581 tgcccgaatt cactccggcc taaccgggag gccattccag cttgcggcct gccaacagat
     8641 gcatgtgcaa atgggggaca gcttccccgg cgtcatgccc attgttgatg accaggcgaa
     8701 agccaccttc atccagatga agagtccggg caatccgccc gactgccagc atcatgtgtc
     8761 ccagaagggc ggcatcttcc tccccggcct cggaaaggcg gggaatgggc ttgcgcggaa
     8821 caagcagcaa atgttccggt gcttgtggac taatgtcccg gaagcaaaca cacaggtcat
     8881 cctgatatac gatgtccgcg gggatttccc nnnnnnnnnn gattttttca aataacgtag
     8941 cggccataaa atcaaaaagg aatctgcaat gactttaaca tgttttgcaa accgtgggca
     9001 agttattccc tgacggctga agcgcgtcgc aggcgatcca taatagcgcg acccaggccg
     9061 gattccggaa cggattccgc cacaatgacg tcaatagctt cattttcatc catctgtctc
     9121 atcagggcaa acaggcgcac ggcagcttct gccagccgcc cgctgccggg actcatggct
     9181 accacttccg cccagtcccc ttcccgggca agagcggacg ttccttcata ggaaagcaag
     9241 ccgtacctca ccccctccac cggggcaaaa ttttcccccg gttccagcag cagcataggt
     9301 ttgcgcgggg cgtaatggct ggtgagctgc cccggcgcat ccgccggaga ttcttccgaa
     9361 acaggacggc aggaaggctt gcggcgcacc atcttgacca ggttgcgaaa ttgttcccgg
     9421 gtcaccggcc cctcacgcag caattccaga gagggcttgc ccttctcgtc aagcacagga
     9481 cgcacaatcg tgctttccag cccctcggaa caggctccgg catccagaat catttcaata
     9541 ctgcccccca attccttctg tacggcagag gcggaagtag ggctgatatg gccgaagcgg
     9601 ttggcgctgg gcgcagctat aggacgcccc agcgctttcg ccaccccccg catggcagga
     9661 tgagcgctta cgcgaacggc caccgtagga aggccgctgg ttacaatatc cggaatgata
     9721 tccgcctttg gcaacaccaa ggtcagcggc cccggccaga atctggaggc aagcgtatgc
     9781 accagatctt tcagcccctc cggaacggcg gtatattttt ccacctcact cccatgatgc
     9841 acatgaataa tcagcggatc aaaagaaggc cttcccttgg cttcaaaaat ccttgctacc
     9901 gcttccgggt tcaaggcatc cgcccccagg ccgtatacag tctccgtggg aatccctacc
     9961 agcgcgcccg cacccagagc ttccgccgca cgggcataca gtttgcggtt gtctgacagg
    10021 ggatctacct cgaacaattc ggtttgcatg gctttatcct tgcctgaaga agctcctgcg
    10081 tcaagcaaga cctccttccc ctgaaaggag aaaacagaca aatgaataag agcacgtcct
    10141 gcggcctcat acttcccctc tctttttcca actgccttta cttcactcca cccttacgga
    10201 cttccccgag tgaaagctgg cgcaggaaaa ggccattccg ctaaaaagtt gttttctcac
    10261 gctaccggat tgagtacaat caagccatga atagggccgc atcaacatcc ttgccaaaca
    10321 tcatcctgat cgggctgatg ggatgcggca aaactaccat cggaaaggaa ctgcaccggg
    10381 aaacgaacct tggttttacg gatacggacc agttaatcga acgccagacg ggaatgcaca
    10441 ttccgcagat tttcaaaatt catggggaat cacattttcg agacctggaa accggaatcc
    10501 tgcaacagct tcagacggcc gccgcacaaa gccgcattat ttccacagga ggaggcatca
    10561 ttatcagacc ggaaaaccgg ctgctactga aaaaactggg cttcgtcgtc tggctccata
    10621 cagacgtaaa catcctgtac cagcgtattt ccagatgcac caaccgcccc ctgctccaac
    10681 aacccaatcc caaggccgtg ctggaacatc tcatgaatga acggcatgat ttttaccagg
    10741 aaacggcgca cttgaccatt gatacggcca atctccacat ccatgaaatc gcattcggca
    10801 ttctggaatc cgccagagta ttccggtccc gacggcaata atgccgaccc gtttttccgt
    10861 gagcgggaaa ttttctcttt ctgattcatc ctaacgcaag cgcttccggg gaagcccttt
    10921 ttcccgctcc acgcacaaga aacaacaaaa ggcgatcccc attaaaggaa cgccttcagg
    10981 aaaataataa tgcaccggaa aaggcttaag ccttacgggc cttacggatc attgaattca
    11041 ccgtttcaga gggctgtgct ccgttggcaa gccatttgtc aaccttttcc agattgatgg
    11101 tatagttgac gccttccttc atggggtcat aggagcccac ttgctcaatg taacggccgt
    11161 cacgacgcgc gcggctgtcg acaacgacga ttttgtagta aggacggtcc ttggaaccct
    11221 gacggttgag tctgattgct actgccatat caattataaa taaaaggtgt taatggaaaa
    11281 cttgcgcgcc ttgccactca catgcgtgcg cgttcggctt tccacagcgg gtgcagatgt
    11341 atgcgatata atggagtcat tgtcaatacc agagttattt ttacaggaaa aaagcctctc
    11401 ctccatttct gcatcaggaa aaattttcca ccgccatgcg gccaaaagaa tacagcatgc
    11461 tgaatccgaa ccccgccagc acaagggaca atattccatc cagtattccg gcataccgtc
    11521 cgtaaaagga acgaatgggc tgccaccgga aaacaagagc ccacaaaatc cagcccgccg
    11581 ttcccgtcgc cacgattaaa aaacagataa aaagagcatc ggaaggagca tgattttttt
    11641 ccaacagcgg agcggaaaga gccacaaaga aaaacgtggc cttgggattc atcaggttag
    11701 tcaccagagc atcacggtaa atacaggaag ccggggaaat atccccgcac ccggcaagga
    11761 aagcattcct ccccctcggc catattttct atgccaaata cagcatccag caggaagcga
    11821 cgcaaaacag ccccaccccc aggaaactgt gaaaaacgga tgctcctact gtacaagcca
    11881 gtgcagcatg ggcaactatt ccggtaccaa tgcccaaagc agccatcact cccgcgccaa
    11941 agccgtaagc cagcgtgctc cgggtaacaa aagcctggtc gggccccgga gatgcctggg
    12001 aaagaagcag aatacctgcg aacataagtt cttccttcat gaaacaaaaa aacccttatg
    12061 ccctgggatg cgcctgtctg taaacttccg ccattctggc acgcgtcaca tgcgtgtaaa
    12121 tctgggtagt ggataaggaa gcatggccca gcagttcctg gacggaacgg agatccgccc
    12181 ctgcctccag aatgtgcgtg gcgaatgtat gcctgatctt atggggagaa atggtaaaag
    12241 gaatggaaga cagcttcaca tatttgttca gcatcatctg cacggagcga gcgcttaacc
    12301 gggttcctat acgggagaca aacaaggggg aattcttcgg cagacaggcc atggaaacat
    12361 aagtttccag agcagccaga gcaggagttc ccacaggcag gatgcgctcc ttgcgccctt
    12421 tccccattac cttcacgccc cggaaacggt ggtccacact gccgacatcc agccccacca
    12481 gttcactcag gcgcatcccg caggaataaa aaagttccaa tatggcggca tcacggtagg
    12541 gaagccatgc gggagcattg gcgggaacgg ccgtcttgta gggaagctcc aacaattcca
    12601 acatctggtt gagtgtcagg aaaacgggaa gcgttttctt ctttctggga agggaaacgc
    12661 ccgtcatcgg attcgtctcc agcccgtgcc tccgcatcat gaaacggtaa aaactgcgca
    12721 gggcggcaaa cctcagccgg atggaagacg tggcggcttc atccttcagc tcctggaaaa
    12781 gccagtccct catctggtcc ggcgtacaat tctcccaccc tgggaatgaa tcagccgccc
    12841 aggccctgaa ctggcgcaag gcgcgggcgt acacctccac cgtatgagga gaagcctgct
    12901 tttccacctc cagatattga agaaagtcct gttccggttc cagaggaatc cccatcatga
    12961 agaataaatt actccgaacc tttatccttg tcatccccgg catccaccgg cgactccttc
    13021 agcagcactt cactaatatt ggcccggatg aacaaatcaa tatcgtccgg ttcttccatg
    13081 gcatacaaag ggctgtcttt atggctgtcc gccagagcct gctctctctc caccgggctg
    13141 agcacaccgt cctggtccaa atcgtaaagc tccatcattt ccttcatttt ggcctggtac
    13201 agagccgcgg catctttcat gacaacggca tgttcctccg gatcaatgcg gccgttcccg
    13261 ttggcatcat aattctgcag caacatgtta cgagtcagca gaaacaatcc gggagcaacc
    13321 atgaagcgct tccgtcccac cgtgcgaatt tccaccatgg gcatttccac cccctctgcc
    13381 ggagggggcg gcggaggaat ttccttcctc tcggcgggag agtaaaacgg aattccgtct
    13441 ctcttgtccg gcccggcatc cggcctgcgc accccgcgcc gcttctctac atgttcc
//
LOCUS       DAWEMX010000062        13497 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1911_length_13497_cov_12.746392, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000062 DAWEMX010000000
VERSION     DAWEMX010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_13497_cov_12.746392"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5714..5723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2700 a         3438 c         4303 g         3046 t
ORIGIN      
        1 ccggggggag ggcgattgtg cgctccggcg tccatggagt gtacagccgt tcatccccgg
       61 gttcctttcc ggaggcggat ggtgcagcgg ttattggctg ttcttcctgc ccgggttgtt
      121 ccgcgggggg ggatttctgg ggtggacagg ttccggaatc cggcggcgcc tgttccggcg
      181 cccctttcag ggcgcgtatg atctgggcgg ggtacggcgt gaacagggag gctgccatgg
      241 ctgccaggca gatgcagact gcgagcttga accaggtgag gcggggtatt ctgcggcggt
      301 gcgctttcat gatgctgaag aaggagaatc attggatgcc ggcaggactt tccgggggcg
      361 cctgcccatg aaaaggcgta tcagccccag aggggcggcg gccagcagca tggcggccag
      421 ccgtattttc atgccgggaa agacccgggc gcgtccgcgg gccagggcac ggaggctgtc
      481 tttcacgacg gtttcaacgg acgtgtcaaa agcgttgcga cccggcatca tgtttccggt
      541 gaagccgggg cggcgcgccg cttttccgaa acccgtagaa accgggcccg ggcagacggc
      601 cagcacgcgt atgccgtgtt cgcgcagttc cagccgcaac gcttccgaca ggctggaaac
      661 ataagccttg gtggccgcat aaacggcaaa atccggaatg ggcaggaggg ctcctaggga
      721 gctgaggttg atgatatctc ctccgttcct cttcatggag gggaccagcg catggcacag
      781 gcgggtgagg ctttccacgt tcagacgcag cagaaaacga attttttccc agcggccgtc
      841 cgagaaatct ccgtaatcac cggcaccggc gttgttaatc agaagggttc ttcccggagg
      901 aaggctgtcc aggcgggaaa tgagcatatt cagggaggct tcgtccgcca ggtcgcaggg
      961 aaggacggag gcgtgaaggc ctgaatggcg tttttccagg gcggcggcaa gctggcgcag
     1021 ggcggcttca ttccgggcga tgagcaccaa ttccgccgta tggggggcca gcgttccggc
     1081 aaacgcttcc ccgaagccgg atgatgcgcc cgtaatgata atcctgtcgt attggaggcg
     1141 tgcggtgtgc atggcgggtt ggagcagccc gtatcatggt caatccggtg cggaaccgca
     1201 atgaaaaagg gggaaggagg acgcaaaaag cccgcctccc ttttttaggc cggggccagg
     1261 cgcagaggtt ttcaggtgca tggaaccggg gccggcctgt tccgcacttc cgggcggacg
     1321 gccgctccaa taaaaaaccg cctgccggat gaacgggagg cggtttgatg gaagagggga
     1381 tggtttctta ggcctgggcg gcttcttcct gcacctgcac tacgcggaag ggcacgcggg
     1441 aggcgagttc gccgtcaacc gtcaggtcga tggagtagtt gcccggtttt tcaaaacgca
     1501 ggccctggaa gttcatgatc aggttgcggg tgaagaaaga ggctccttcc gggaaagcgg
     1561 gaaccgggaa attgatcttg atgggcatgc gttccttgtc cagcggcgtg ccgtcggcat
     1621 caacgatgct caggcccagt tcgtgcgtgc cttcgtcttc aggagtgagg cagatgcgca
     1681 gggcaacggc gcagacggga tgcacgaccg ggaactggcg ggcgaccagg gagtcaaagc
     1741 cgccctgaat gcagagcttg ccctggtagt cggcggcgaa gtcacaaaga gtagaaacta
     1801 aaatatccat gttgtgataa tgttgatgta tttggctagc tttcacgcgg aaagcatgaa
     1861 cattggacag aactatactt cctccggagc tattgcaaga taaagctgta gaaaatattg
     1921 tcatttctcc gtgaaattcc ggttgcgctt gagatggggc gggaatccct gtactactgt
     1981 tccggcaacg cgtttccggg catgatccaa tggttttttg acattccttc caagctgatt
     2041 cactggaggc tgaatctggt cagggcgctg ggcgcctacg tgccgtggct gcgccgtgac
     2101 gacggctcca tgaaggtgca tgccggaacg ctggtggact tgttcgccac tattttaagc
     2161 ggacgccgcc atttgagcgc ccgtgatgcg gacgccgctc tggatattct gcgctatact
     2221 ttcccggaag tggagcaccg ctggctttcc aaccgttttg agcggtccat gagggccagc
     2281 ctgacggttg cggatgtcct ggcctccgca gcttccggca gggatgagac ggaacgcatg
     2341 gcgatagctc tggaagtgct ttccctgctg atcaatacgg gagattcccg catgacaggg
     2401 gagctgtttg accaggtgac gtacggtctg gaactgccgg gcacggcaaa ccacctgagg
     2461 cagctgctga tgacgccgga cgtggaagcc caggaaccgg cgtacagcgt cggtttttcc
     2521 tccggcatcg gtgcggaggt atctcttccg gagcaggaca agggcatttc cttccgcctg
     2581 atccggtgtt cccgcctggt gctggtggtg aatgacggca cccgccccat tgtggtgcgc
     2641 gggcgccatt tgcagcccgg cggggtgatg ccgctgacca acgggcaggt ggtgctgctg
     2701 ccgtccggtc cgctgagttt tgaagacttc gcgttttttc tggactgcaa gcgttcaggc
     2761 aagcaggaag tatgctatat ttccctggac ggcggctccc tgcaaatcag ccgggtgcgt
     2821 tcccgcgcca gcgaggtgcg cgtgaacatg gggctggcgt gcgaggtgga ggtgctgagg
     2881 ccggaagtgg aattcgcggt tgacgggaga cggctgtacc ccggtgaaag cgtgcgcatg
     2941 gcttattact cctccttcac gctggacggg gaagggcctt tctccatggg agaggtgcag
     3001 aattccatgt cggacatcgg gcgccgtttc cgcctggatc ccggaaaccg caaactgcgg
     3061 gtgaccaacc tgccggaaaa ggcgcgcaag ggagacatgc tgctgactcc cggtctggcc
     3121 cccggagcgg tgctggaggt ttctttttcc agcgccacta attccggctg gctggaagtg
     3181 gtggaggcct ccatgcccct gctgctcaat ggccggcctg ttcggggaag ggtttccctg
     3241 agggatgggg acgtggtgca cctgaatgcc taccacgcgc tgcgctgccg tttttccgcc
     3301 ggggtgctgg atgaagaata ccatgcgatc agaaccctca gcgtggaagg agtgaccaag
     3361 gaattcctgc gctccgggcg cgtgctggac aatattgacc tgaccgtgaa acgcggagaa
     3421 atggtgtgca tcctggggcc cagcggatcc gggaaaagca cgctgctctc tatgttggcc
     3481 ggccagctgc cgcccacccg gggctgcatc cgttacaaca accagcttct gtacagcgcc
     3541 ccggatctta tccggcccta catcgccttc attccccggg aagatattct ggatgcggcc
     3601 atgagcgttt ccgaacacat ctctcaggcc accatgatac gtcgtccgcg gctgagccgg
     3661 tcggaacgcg tcaggcgcgt gaacgctatt ttgaaatttc tgggcctgac gcacatcgcc
     3721 tcccgccgcg tgggtgagat gaacgcccgc accatttccg acggggagcg cacacgcctg
     3781 aacctggggc ttgacctggc gggcattgcg gatgttttcc tgctggacga acctatcagc
     3841 ggcctgtcca cgtcagatgc caagctggtc atgcagacca tgcagaacat gagccgcgac
     3901 aaaatggtaa tcgccaccat gcaccggccc agcacggcta ttctgaacca gttccacaaa
     3961 gtgctggtgc tggaccacgg cggccagatg gcttactggg gggacgtgcc gggcatgatg
     4021 cggtatttcc ggaaggcggc agcggacatg gctattgacg tatccgagga ggctcgaacg
     4081 gccggaggcg cggactacgt gtttgaggtg ctggaagccc ccctctcctg gcacgaccgc
     4141 cgcaggcggc agcatccccg cctgtggcag gagcgctttg aaggctaccg gttccgcaac
     4201 gtgatggggc atcatcatga agggggcgcc cccagaacgc tgtatgaagg cacgctggaa
     4261 tttccccccg cccccaggcg cagcgtcata cagctctggc ggcttttccg catctgggcc
     4321 gtgcgcacct tcctggggcg cgtgcggagc cgtatggggc tgtacaccat gctgctggaa
     4381 gggccggttc tagccctgct gatttccatg accctgcgcg cctcttccag cccggaatat
     4441 acgtttgcca cggccctcca catcccttcc tacctgttcc tggcgaccat tgtggccatg
     4501 ttcttcggcc tgaccggggc ggccagcgaa gtgctcaagg accgtgccct gctcaagagg
     4561 gagagcaatg ccaaggtatt cgtgaccggt tatgtgctgg caaaggcgct ggtgttgacg
     4621 gggctttccg ccgtgcagtc cgccctgttt ctttgggtgg ggaactccat tctggaaatc
     4681 catgaaatgt tcctgattta cctggggacg atgaccctga cggcctttgt cggcgtgagc
     4741 ctgtccctgc tggtttccgt cttcgccagg acggagaggg cggcgcttaa catggtgcct
     4801 ctgcttctga ttccccagat attgatggcc ggagccattg tccgctttga tgaaatgaac
     4861 cagttcatcc catggtccgc ccaccgtacg gatgaacacg ggcgtcttaa gccggggcgt
     4921 gtgccgctgg tggcggaatt ctgccctttg cgctactctt ttgaaacgat ggtggtggac
     4981 caggcgtcca aaaatgtatg ggagagggag cgtgaaacca tccaggagaa agtggacgac
     5041 ctgaaggaac gccctcatct ggacgacagg gaatttgaag aattcaagct gctcaagatg
     5101 gcgctgctcc acattgccgc tattggagcg gaggggccgg aacaggccaa agccgctgtc
     5161 cggcaggtga ggagggcagc ccttggcggt tcggaaaaaa gctacaggcg gactattgcg
     5221 gaattggaac tcctgggtaa ggaccgtccg tccgttaaat ccttctatgt caatgaccgc
     5281 gttgtgatgc tggatgaatc tgccgaaatc cagcgtgtca gccgggatgc cctggacagg
     5341 ccggaaattt ttctggcccg ccgccagcct ctgccctggg gaggctccgg cctgtccccg
     5401 ggggattccg gatacagcgc ggatgacgga acggttccca ccccatggaa ggattccctg
     5461 ttcctgttcc tgatgggggc tgcccccctg ctggtcacgg gccgcgtcct caagcgcagg
     5521 ctggaaaagg ccggatgacg tttgcggcct gcggggattg gcgtctggat tttccgccgc
     5581 aaagcggaaa atccggcgtg cacgtccggg gaatggcagc gggccgtaag ccccggtgca
     5641 tcccgcaaaa aaaacggaag gcccctccaa atattctttt atttatgtgt tgacgcgccg
     5701 gacggaatca ctannnnnnn nnnccctttc cgcggcagcc ttgtcccttg gactcttttc
     5761 ctgcggaggc agcggcagca gcggtgacgg ggaaagcctt ccttccggca ccatgctgga
     5821 cttgagcgct tctctttcca cggaagatgg gctagggccg tggaatatta tcgtcaaatt
     5881 gacgggcaat ggttcggacc ttccccaggt aagcattaat gcggcaagca gcaatgaaag
     5941 tgctcctatg aacattgctt ccgtagttta ccgcaggacg ggatcatccg ctattctgag
     6001 ggggaacttc acgattactt ccgggcagtg gacggctccg ggcccgattg tcgtgacaaa
     6061 cagggacaag gcctttatag atgctaatat ttccttcagt tcctccaatg aacgtgagag
     6121 ctgcggcggg acgttcaccg gttccatcac ttaccaggtt cccgttgaga atgatgaagg
     6181 cgaggttgaa gcgcaggact ggaccgctac ggaaggaacg gtgagttaca cggtatcctg
     6241 agttctgatc atttatgacg gggctttgct gccgtgaaac ggggggcagg cctgtttgaa
     6301 attatggtcg gaagggcaaa gaaatgtctt tggggcatct ggctgtgctc ggggctggtt
     6361 ctttgtgtct ggaatgcgga atcggccggc gggggggctg gtatggcggc atcgaaagat
     6421 gcctgtttca tcaagacttt ccgcgccaag atcgtcccgg aaaggctgcg ctcctttgtc
     6481 atgccggcag atggagttgt cagcaactgg attcccgcgg acgggcgtgt tcagaaggat
     6541 accattattg caacggtcaa tgaagatgaa attgagctgg agcgcaagga gctggaggtc
     6601 aaaatcctga aggaccgcat cgccaaggag gaagagttgt caaagctgga aaaacagctt
     6661 gaagagatag aattctactc ttccctgtcg agggaggaac ggcaatatgc cagcaagcgg
     6721 gcggaaggcg gggaaagggc catccgggct ttgaaagaca agattgagct ttccaaaagg
     6781 gagttgtccc tggtggagga caagccgcgt ctggattttc gcaagaagga ggaaaaatat
     6841 atcctcagaa tgccttttga cgggaagctg cagtaccagt tttccattcc gccggataac
     6901 agcgtagccc tttacatgga cgccgcgtcc ccgattgcga ccgtgtgcga tgactcctct
     6961 tattatctga ccatttccat ttcggaccct gatttgacga atctgccgcc ggaatccctt
     7021 tccctgtccg tccctctggg cgacggttcc gccctgaagg gggtttttgc cttcaagcgg
     7081 gtggagaaga atgtttccgg aggcggggaa ttgctggcgt attttttccg tttgccccgt
     7141 caggagcatg cgcgcgccca ttccatgctg ggttccaact gcacggcccg tctttattac
     7201 atgccgcagg gagaagtgtt tcatctcaac aaggtgaggc tggcttccct gccggaggga
     7261 aaaaaatgtt cgtcctggca ggaacttctt gaaaaagtgc atccggacct ggagctggtg
     7321 gtgaatgggg aaacggaatt gattgttcga aaaaaatgaa attgctgtgc gaatcagtct
     7381 gtttcagtta ttcaggcaag cagtggattt ttgaaaatta caacagggtg ttcacttccg
     7441 gaatcaccat tttgaagggg tattccggat gcgggaaaac gacgctgtta aagattctgg
     7501 cgggttatct gagcccgcag cgcgggcgcg tgattacccc ccgccgtaag gttcgggcgg
     7561 attccctcta ccgacgcaag gaggtaagtt acatgttcca ggggctcaat ctccttcccc
     7621 tcgctacggt ggagagaaac cttcaattat gcgcggaaat ggctatgctg cctaaaaagc
     7681 agtggacgcg gagggcggat gatttgattg cccgcctggg cctggaatct ctgaggcata
     7741 aaaaggcggg gagcttgtcc ggcggccagg cccagcgggc ggctcttgcc cgcacgatga
     7801 tgaaggattc cgatgtgctg ctgctggatg aacctacttc cggcctggat gacggtaata
     7861 cggaaatcat taaaaatttg atcagggagt accctgctga caaaatttgc attttgagca
     7921 cgcatgattc ccggctgttt gacctgaccc atgaaatcat tgattttaac ctgtctgtat
     7981 ctctttaaag atactctcca ccgttggaag gaacgcccgg ccagccccct ttcacggatt
     8041 ctggtggcct tcttcctttc cctgtgcgcg ctctcttttc tcgccaatta tgtcctctcc
     8101 gccaaaatgc tgcatgatga aatcaggaag aacggggcgg acctgattat tgtttttgat
     8161 accgtgcgcg acggtgagcc ttccaagaaa aatttgctgc agcatgaact gccttccctg
     8221 tatggctgcg acgtcctggt gctgaatgat gcccgtgcgg gcgtggcgcg cgtgggcaag
     8281 acagtattcc ccatcctgga atacaacatg gagtcctgtt cctttctttc cgcgcttggg
     8341 ttggaggacg tggctaccgt cctcctgtac aatccctccc ggagcccgtt gcaggcgggg
     8401 ccttgtacgg ttaccatagc ggattttgat ttttccctga atgcctcccc tctgccggag
     8461 ggtcatttgc tgggcaggct gtatccttcc ggagtcattt tggccccgga aggaactttc
     8521 agttccggac aggaggtccc cgtggaaaat tgccgctaca tcatccgggt gcatgaaatg
     8581 acggcggccc gcattcgggg catagaagaa gtgctgaaca acatggtgcg ttatgacggc
     8641 agcatgacca tggttcagac tcacgtaggg ctgcttgaca ggctggaagt gctgatgggc
     8701 aaccagacgg agtgcaggac tgggttttcc ctcggcatcg ccgtgatcgt cggcattctg
     8761 cttacagccc tggcatccat ggaattccgt cagaatgaat atgtctatac gctgatgaag
     8821 agcttcgggg tgcgtcctgt actgctgatt atggcgttta tcgtggaaaa cgcgtttctg
     8881 attggcgttt cctttgcggg agccgtctgg gcgttcatga aaacccagag aatcgtattg
     8941 ggggaattct ttaagctggg aaactccgtc ctgacttatg ccgatatacg gcaggatttg
     9001 tggctgctgg gaatttccct ggcggggtgt gttcttcttt cctccattcc ggttgccttt
     9061 tccgcctgcc gccccatagg gaaagtgctt aagtaagtga tggggcttcg gggctgttcc
     9121 cggttttgtc tgcgttccgg catgtccgcc tttgccggcg gtctggggga aaggccgtct
     9181 ctgttccggc aggaagagat aatggataaa aaaatccctt cccgccatgg gggcgggaag
     9241 ggaggtagga agaaagggtt tattgatcag agctgggggg cactctgaaa tccctgatga
     9301 ccgcctggca gttatcctgc tgggtttccg tttcagtata ttcggcgtct ctgacgctgc
     9361 aggtggcaga accgatgcct ttggaaatat tctggggatt atacaggaca acgagggcat
     9421 ccagattgct ggttgtttcc ccgtcattgg acgaccagta aaagcctatc tgttccgcag
     9481 agtcggaagc tggtttgtag ttgacgacat gaagggttgc cgttgaggcg gccgggctgg
     9541 ttccggtgta aatggtggcg gtgctttctg aggcgatgat gaaggagaag acgtaaggct
     9601 tgccgtcctg aagcgtgatg tccaacgtgc gctgaagacc aatgggaaga taggcctgtt
     9661 cgctggaact ggatccgccg cagccggaga ggagcccaag cccgagagta attaaagcgg
     9721 aaatataaag gcgggaattc atgatttgat ggataaagga aagaatttgt ttatgactgg
     9781 gatatgatgt tgacgctgat ggtgatgccg tcgatgggga tgggtgtcct gtcttccgct
     9841 tgcatgtagg aaatgctggc gccgccttcc ccggaagaag catgggtggc gttctcaaag
     9901 gttaactggg gaatcctgat ggtgctcatg agagtgttgg gttccgagga cgaaccgctg
     9961 tttctgttgt aggacaggct cagctccgca gtgtttttcc cggttttggt gtaggaataa
    10021 atgcctgccc cgttccacat gttcatgttt tccccttgcg tctggatgat gaatgccccg
    10081 ttgtcaccgg tggtcatgtc aaaaatggcg ggaagcgtta tggcggcatc cgtaatgcct
    10141 gttacctgca tgtttaacac cttgctgccg atggtagagg gcgccaggcc ttcggcatca
    10201 gaactgcttc cgccgcattg ggccagcgta agaatgccga gggagcagca ggccgcggac
    10261 aaagaggtaa gaaaaggacg catcataaaa ggataataat tttccgataa actgtgatat
    10321 cacacatgat ttgtcaagat aggaatcagg agggaggtat tttttacacg gggaaaggaa
    10381 aaaccgaatt tcctgtgctc caggacttct ttttccttct ttcccctcct gccgtgttga
    10441 tgcttgactt ttcaggggca gaagctatgt aacaaggggc ctgtatgttc ccgtttcctc
    10501 ctgttgtccg ttctttcctg ttttccctgt ttgaaagggc atggctgagg gctgcgttgt
    10561 gcttctgcct ctgtacggcg gtatgcgcct cctgctccgt ggacaggcac atggaaaaaa
    10621 aggcggggga attgatggcc aggatggatg ccgttcccga ttggcggcag ctgccgcgga
    10681 aggaaatctc atggcaccag gccctggcga tgatgatgga gcggaacatt gacttgaaaa
    10741 aatcggagca gtccctcaag acgacgaaac gttccgtcgt taatgttttt acccagatta
    10801 tccccggagt caatctggac tggatgctga ccaaggaatt gagcgacctg gccagggtga
    10861 cggccagcga tgtggaatac aatacgaaca ttctgttcaa catgccgtcc ctcacccaga
    10921 tcccgtttga ttattattcc gccaaggcgg ctgtttatac ggcggaaaag acgctggaga
    10981 tgaaaaaaag ggagctggtg gccagattgt accagcaggt gctttcctac cggaacgcgc
    11041 agatcagcta caataaccag ctgagctccc tgccttatga cgatgatggc gtccagaaaa
    11101 agaagctgga cctggaacgg gagcggaatt tgaatgagat ttcccagggg tttgccgtgt
    11161 tgctggggaa tatggacgcc cggtggctgg tgaaccctgg aacgatgccc aggctggact
    11221 ggggcaggta cagggcggcg tcccggcagc tggatctgct ggtggtgacg atggtggcca
    11281 tggagctgga agcttcccgc ctccaggtgc tgaatgccaa actgaagttc ttcccgtccg
    11341 tggatattaa tttttacagc ccaaccctgt tttccagcac gggcgggacg tacggcggtt
    11401 tttttgcggg agcgggcgat atgaaggtga acatgagcct gcgggaagaa ctggatacgc
    11461 gcctgacatc ctggtttcag tataagtcgg ccaaagaaag ccacgaactg ctgcagcgcg
    11521 aagtggtgat ggaactgcag cggcggcgca tcaagatagc cgcgttgctg gagagccgca
    11581 ggaggtttga gctctggcag ggggtgctga tgaaggaaat cgcgttcaag gagtccagat
    11641 tatccgtttc cggagatgaa tatctggaac agaggaagga tatcaggaaa atgtatgctg
    11701 acctggataa tgaggcttcc aagaatgcgg aggtggaagc ggccctcatc atggaatatg
    11761 gctggctgaa gtaaggcggg ggaggatgaa caggccctgt gtgactgcgg gggcttcctt
    11821 tcttgatgct ttcaagggct tgtgctatgt tccggatatg caaaaagttc ggcagcccac
    11881 gggtacggca ttgatgattc tggcttttcc ggccgccatt tacggggcgt ttccgtgggg
    11941 aattgccctg ggggcctttt tgttcctgct gggggtggtt gtggcggcca gccgtttttc
    12001 cgccatagtc tggtcatggt gcctgttttc cctggggatg tatctggcgg ctccttttat
    12061 ctggatgtgg aatccggact attttttcag ccgttcgttt gacattctgt tgataagcgg
    12121 ctgcctggtg ggcagcctgg ttctgcttgc aatccggttt gcggttattc ccgtgctgtg
    12181 gcccaggatt ttccgccact tcgtggaagg aagccctacg ctggaggatc tggcgcccga
    12241 aaagggaaag acggtggctg aactgcgggg cggcatagaa cgcaggcagg tgaatttcct
    12301 gttcgggcat gcggaccggg aactggtaga aaaatggggg aaaatagccc agggaatagc
    12361 ggctctttcc agaagccgca aacggctggc aatatgggct gcgggtattt tcctggcttg
    12421 gcccgcagcc gtgtttttcc tttccggggg gatgggcggc gcgctggacc gggatattga
    12481 ccgcatgtgg ggaacccgcc cgttccggga tggcatggtg gtttccagcc ctgcggcgat
    12541 gaatgcggcg cggcgcgttt tcagggatgc ccgggcgctg cgtggcctga cgatggagca
    12601 ggcgcgcgcg tggctgagga cggaccgcag gagtgcggca ttcaaattgg gcaagccccg
    12661 ttatgatttt gaaaaggatg aaaccgtgct ggtgctttca gatgggaggg attccatccg
    12721 cctggtggcg gttttcaatg atgcgggtca gattacccat tcctggcctg aggagtatat
    12781 gactttgatg gtcagggatc tggagaaaaa ttgaatgccg aggccggaag ggggcattgc
    12841 ccggcagggc atccggtgat tgcggaaggc cctgcggcct tccgctacag cgtgcgcttt
    12901 tgcggaggcg tcaggttcag cctgccttca ctgatatgga tgcccatgcc gctttcttca
    12961 atcatgtgga taagcggcag gtaggcctgg agcaggtttt caaacgcagg caggagaaaa
    13021 atcatgtaac cctggggaac ggaaaggctg ccgatggtgc cccccacgtg gattcctccc
    13081 ggcagcgttt cgtcattgta gaattcgaag cgggtctgta ccgccatgta attgtgcggg
    13141 tccagggatt ggaagggcgt caggaacagg gaaatcgtct ggcgggagtc gatgcccgta
    13201 tagcgttcga tgatgatttc catgtatcct tccccgatgg ttccgtccgg ccgttccttg
    13261 ccgccgtgca gacggatgcc ggccgccggg ttggtggcga agatggccag ggcgccgtgc
    13321 tgctggggat ggatggagga agccaggtag cggttgatat cttcttccga gaacgagacg
    13381 gaggcgtccc tgttgatggc ctgtttcagg attctgggga tatccggggc gttttccctt
    13441 tggcggaagc cgggcaggtc gtccgttttc tgcgggcacc aggaggagta aaccagc
//
LOCUS       DAWEMX010000063        13458 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1925_length_13458_cov_10.902037, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000063 DAWEMX010000000
VERSION     DAWEMX010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13458)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13458)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13458
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_13458_cov_10.902037"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11406..11415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12327..12336
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3250 a         4058 c         3492 g         2638 t
ORIGIN      
        1 tccgcctcca cgccctgccg gaaaggcaaa ataattcctc tcttcgccgt caacatgcgg
       61 tccaatgccg gctcacctca cggcatgcca tgcgccgccc cggcaataat gaaaaaaata
      121 gatgggaaat cccgccatcc tgatgtagca ttcccaggta attttccgct ttcccccttt
      181 ccggggaatc ctgcttccct gccatgaacc gattctttct atcctgggac aaaccggcct
      241 gccgtgctgt ggcggaacgc ctgctttccc tgggaaatgg cttccatcgg catctggtgc
      301 tggttcccac acgggaatcc ggaaggcagc tgagggaata ccttgcctcc atttcccgta
      361 cacaggcaat ctttgctccc caggtcattc cggcagacca gttcctccgg atggaagaaa
      421 aggaagaaac ggcgtccgct ccggaagaac tggccgggtg gctcctggcc ctggggaaaa
      481 cgcctcaccg cctttatccc cgcctgtttc cacgcaccat gccggaggat ttttccagca
      541 tgctggaaat ggccggcagc ctgcaaaacc tgaggcacgc catggcgaac cagggcgtct
      601 cctgcatcat ggcccaccat gcctgtgcgg gtagagacga acgctggaag gacatggaaa
      661 ggctggaggg acaatgcacg caacaactgg aaagctggaa gcttgaagac aggaccgcca
      721 tgaaggcgga agccccgccc cggctgctga attccctgag ggaaacaggt ggaaacatta
      781 ttctggcctg cacagcggag gtgccgcccc ccctgcgcca tgccctccag catgcggaaa
      841 gaaatggcgt acccgtccag atatggatac atgcgccgga agaagaagcc gcctcctttg
      901 attcctgggg atgccctctg ccggaagagt ggtcccggcg ccccatccca atccgcgacg
      961 gtcaaatcag aattgcggcc aacccggcgc ggctggctga agaaacgtgc cgcatcatcg
     1021 cccgcacagc ggaaggagga acgccggaca tcgccctggg cgtttgcgat cctgacatga
     1081 acgtcgccct ggacgcagcc cttcgccaat acggctgggg cctccacaat ccggaaggga
     1141 agccttttgc cggaagcggc gtcatggatc tgctccgcaa ccttcggcgg gcactggaag
     1201 aaaaggggac ggcgcgcccc gtctattccc tggcgcgctc ctccctgctc tgctcctccc
     1261 tgggaataaa aggccagcag ggttgctgcg ccgcgctgga caaaatccag cagaaattcc
     1321 ttccggaaac ggaagaatac ctgctttcca ggctgaaaga ggcttatccc ggcgcctttc
     1381 cttccatcca ggccattctg gaatggcgga accggatggc ggaaccggga atgctggggg
     1441 aacggctgat ggaatggttc ccggccctgg cagccatcta tggcccggag acggaagcca
     1501 tggaaatgtt ccattcctgc ctttccggac tcatgcgtct gcaacagcgc tccaccgcgt
     1561 tttccagccc ggagacggct ctcctgctcc tgatgaaatg cctccagccg cttcgcgtca
     1621 gaaacaggag aaaagctcat gcggcactgg attccctggg ctggatggaa gttcacttcc
     1681 gcccggaaaa aaacctcatt ctcacgggac taaacgaagg gaccgtaccg gaaggaggcg
     1741 tttccgacca gtttatgccg gaggaactgc gggaaaccct gggcattgat tcctttaacc
     1801 ggaaaaaagc ccgggacagt tttctgctga ccgccctgct ccattcccgc gaacgggaag
     1861 gcagtctcac catccttctc tcccggacca gcagcaggaa cgatcctctg accccttcct
     1921 ccctgctcat gcgctgcccg gaggcggagc tgccgcaccg cgtagaacgg cttttccagg
     1981 aaatcagcga tgtgcccgct cccctgccct accagcgcgg aaactggcat cttcaacccg
     2041 cggaaggctg gaagaccgct gcggacatcg gcgcgatggc gcccggctac aaaaacccgt
     2101 ggaaggaaaa agggctggcc ttctccccct ccgtcctcaa aaggttcctg gcctgcccca
     2161 tgcgcttctg gatgagggag gcgctgcaca tgaatgagga agaattcctg ccggacaaag
     2221 aggacatggc cgtcaacgag ctgggcacca tgctgcacga cgtgctggaa tgtttctgcc
     2281 gggaacacgc cgtcctgaaa gacggcatga acgcagcctc ctttcagaac gctatcacgg
     2341 aaatactgga acaaaccttc cggaaacaat acggcccctc ccccctcatg cccctgctgc
     2401 tgcaaaaacg ctccatggaa cagaggcttt ccgtttacgc cgttcagcat ttgcaggccc
     2461 ttcaggaagg atggtcctgc attgcctttg agcaccaggt agaaaactgg atactgggcg
     2521 gattccccat gaaattccgc attgaccgca ttgaccgcca tgcggacggg cgcatacggg
     2581 tcattgacta caaaacggga gcggcctcct cctgtgaaaa aaagcatctg gaaccgctcg
     2641 gacggccgga cgccttgtcc ctgctttccc cagccctgca tccctacacc aaactccgga
     2701 aaaacggaaa gctggcgcat gcgcgctgga aagacctgca gctacccgtt tatgtgctgt
     2761 gggcctcgga aacctacggg ggccatccct ctgcggctta ttacgccctc cccgccaatc
     2821 ccatggacat cggaatctcc tcctgggaca ccctccacga caccatgccc gggtatgagg
     2881 aatgcgcgct ggacagcgcc tgttcctgga cggtggagct gatgaaactt cttcatgaag
     2941 gacgcggtct catcactgcg gaggaactgg gctggacacc gccctcttat gacgtattca
     3001 aggacctgat gacttcccgg agggaaagcc tgcaggatct gctgggcctc cacctcaccc
     3061 cccacctgcc tttctaagat gccgcccctg accaacatgc tgatctccgc ttccgccgga
     3121 acgggaaaaa cctaccagct ctccctgcgt ttcctggggt tgctggcgct gaacagcggc
     3181 aaccaccccg aacgcctcat cgccattacg ttcacccgaa aagcggcggg agaattcaag
     3241 gaccgcattc tgacagacct ggccgcgggg gccacggatg aggctggcgc ggcgcggctg
     3301 aaagaacggc tctgggcggt tatcaaggga acggacgggg agcccggtct ctggcccggc
     3361 gctccggagg catggaagga ggaaaatctg catcgggaac gcttcctcca tttactgcac
     3421 atcctggtac agaatttggc gcgactcaac ctctgcacca ttgacagcct gtttgcccaa
     3481 attgcctccg ccagcacctt tgagctggga gtcagcggct tcagtatgat tgaccccacg
     3541 gcggagaaac tggcgcgccg tgaagccctg ctctccctgt actgggaatg ctccgtaaac
     3601 agggagcgaa ggaaggattt tgaagacgct ttcctttccg gggcggattc tgacgcagaa
     3661 gccgcagacg cggagaaatc catggtgcgc cgcctgggca cctatcacga actttttctg
     3721 gatgtgccgg atgccgggat gtggggaaac cccgtcacgc tgggcttcac accggaggag
     3781 ctgacgcctc ccgtctctct ggaacaattt gattccgtcc ttcattccct gattttccag
     3841 gttcagcaaa ctcctgcgcc ggaagggaaa aacggcgtaa aaaacaaaga actgttcctg
     3901 cgcttcctga acggattttc ccaatacgcc aggctgggcc gcgtgcgctt ccgtacagag
     3961 ggcggttccg cctgggctat taccgtggaa gaggcgcggg aaaaattccg ggatttctgg
     4021 atgcctgctc tggaagaact catccaaagc tggcttcgca tggaggccct gcacacgctg
     4081 cgccgcaccc gcgccacgca cgggctgatg ctcctgtttg agagcaaata ttcctccctg
     4141 gtccgcaaca gggggagatt cctgttccac gacgttacgc gcatgctggg agggggtacc
     4201 atgacgccgg aactcaaacg ggacctgcaa taccgcatgt actgccgcta tgaccattgg
     4261 atgctggatg aattccagga tacgtcccag ccccaatggc gcgtcatcaa gccttttcta
     4321 gatgatctgg cggaatccaa aaccggaaat gaaggcagca ttttcgtggt gggggacatc
     4381 aagcagagcg tgtaccaatg gcgcggcgga gacccggagc ttttccgctc cgtttcttca
     4441 cagcttcaac tggaacaacg cggcatgtcc acttcctacc gttctgtcca gccagtgctg
     4501 gacctggtca acgatatttg cgactatgcc cggacagcgc ccgggtgcga acccgccgcc
     4561 ctggaacagt ggggagaata tccggcgcac cggtgcgccc ctcatctgga agaccgtccc
     4621 ggcacctccc aaatctggca ggcgcccaag gcggaaaacg tctccgccaa caacgacctg
     4681 gtatgccagg cggcggccga tattctggag cgtacagggg ccctgcgccg cggcctggaa
     4741 acagcgatac tggtcagtac gaaaaaccag gctcttgtca tcaagggctg gctgacggat
     4801 cacggcatcc ccgcagaagt atgtgacgac gttccagtag gtgtggactc tccgctggga
     4861 aaaaacctgc tgtacttctt ccgctggctg ctgatgccgg gagacccctt tgtcatcggc
     4921 ctgctcaccc actcccctct ccggcccctc atcacgcagg gaggcccgga aagcatgggg
     4981 tggaaggaat ggcgcctcct gctggaacgg gacggttacg ccgccgtcat ggaggaactg
     5041 gaacagcgcc tgctccgggg aggggcggaa ctgacggact tccaccgtga ccgcctggcc
     5101 gtctggcaga atgaggcgga acaggtggac gaacagggcg tttccctgga tgaatggatc
     5161 aggcgcatgg aagacctgac ccgccgggaa gaccccgccg cgggaattgt ccgcatcatg
     5221 accatccaca aatccaaagg gctgggattt gacatcgtca tcctgcccca gatcggcagg
     5281 gatacacctt ttgcggacgg aaggcatctg acccatttta tcaaaaaaaa cggggagggc
     5341 ggtgtggaag gcattgtcct ggccccctcc cggcatgtct atatgaatat cccgcaattc
     5401 cgggaacttt acggggaatg gcgcgcccgg cagcaattcg acggattctg caagctgtat
     5461 gtagcgctta cccgggccaa gcgggctacc tacgttatcc tcccgtaccg ggaagacaag
     5521 gaagaaacgg aggcagattc catgtggaaa gtagtccgtt cctctatccg gcccctcaac
     5581 cggggaacgg aagacatact tccggaatcc ggagcgtcat gcctgtactc ccgcggtctt
     5641 gacgggtggt atgaggaatt cccggaaagc atacaaaaac gcgcggagaa aaacgcgctg
     5701 gaatggcctc cgcaaaaacc gttggccaga gaacggatat ccccttccgg cctgtcggag
     5761 gaagcctccc cgctccaggg agaaaaacac gcgggcgcag ggaaagccgc cgcgcttggt
     5821 tccgccgtcc atgccgtgtt tgaacggatt acccgctggg atgatgaaaa caaaccggca
     5881 tgggctctcc accctgccac ggaggcggaa cgcatcgtag cggagtgcat ggaaatccca
     5941 tccatccgcg aacttttcac gcctccggaa acggcgcgca tcatgaagga acagcgtatt
     6001 gaagccattg acgggaataa ctggatttcc ggcatcattg acaggctgat tcttgatgga
     6061 ggcggcgccc gcatcgtgga cttcaagacg gatcatgccg atacccctga gcaactgcgc
     6121 gagcgccatg caaaccaatt gaacgcttac gcccgcatcg tgtccagaat taccggaata
     6181 ccgctggaac gcattacgcg caccatcgtc tccacctgct tgaaggaaac cgtccccatt
     6241 caataacccc ctattcccgg acaagtccgt tttcccgctt tttcccgcgc ctcattccaa
     6301 ttccggctct tctccggaat ggggcaggca ttgctccggc caacaggccg ggatagaaac
     6361 gacctgctgt atagacaggg aaaggaaaaa cgccaaataa aaaaaccgct ccgcgtgaat
     6421 tacggagcgg cgttgataac aaaggtctgt tctttccggt cagtccctgc ggcggcgcag
     6481 gaacagggca gcagcgccca gcaggctcaa tgctgccgta gcgggttccg gaacctgcgt
     6541 cagggaaagg taattaaggg ctgtaaggcc gtaccccttg cttccatccc cctgaatcgt
     6601 aagggtaagc gtcccgtctt cccctaccac gacattctct accgtagcat aaagcccttt
     6661 attggcagta gcgctggaag tggaagcgtt cgccggagta aggactccgg aattatcgcc
     6721 cgtcacgaaa gaaatttcgg cccagttccc gctggcgtct gtgccgaagc ttgtccagac
     6781 ggcattggaa gcatcggcgt ttccgatcgt ccaggtttga ccggcaaatg catccttgcc
     6841 tgtgaaacct ccaaagatgg acaaggaata ctccccggcg gcaagaccgc tgaacgttac
     6901 agtgaaagag ccggacccgt cggccgtctg cgttaaaaga ccgtcacgaa aagtgtcccc
     6961 caagctgaaa ggcatgctgc cgttgaaagg atttttccaa tcggcttctt cctcaactgt
     7021 cttggtggtg gtatggccat tatcccctac aggccattct gccgtactcc atgccaaaga
     7081 aacgcttccc ggcatgctgc ccaaagcggc ggaggcacta tcataattca tattgagcac
     7141 gccgtccgtc gtggcgtcac tgcgtccgaa atcaatttgc accgttgccg cctctacggc
     7201 aagcaaggta cttgagatta aagctaaaga caacaaggat ttcttcatgg caaaacgaag
     7261 taaaccgaat taacaatccg atgaatgcca agttgtctta ataaaaacat attttattca
     7321 tattaaatat gttatatatt tctatcatat tgatggaaga tgatttatcc caaaaaaata
     7381 aacgtaatac atgattaaat tttcatgatt tgccattgac accggattgt taatccgcgg
     7441 acatttgccc gcattcaaat aaaaaacgga aaggatttgt aaaacggcca gaataaagca
     7501 gtcacggttc cggaacagcc acccggatgc tttcaatccc caaataccgg gctacggaac
     7561 gattgataaa ttcatccgga tccccgctgc tcatacgcca cataacggaa acataagggg
     7621 ccaaaggaac ggaatcataa ggaggaaccg taatgttttt cttgccttgt tccaattcat
     7681 ggcggatgaa cattttccgt tcctcgcact ggcggtggat acggggaaaa gcatccagat
     7741 atccggagca tacccacagg gccggaccag ccgccgccag cagcacgcag aatgggagcc
     7801 accgccttcg cacggcatcc atccataccc cgtacaatcc cagaattccc gtcatcatca
     7861 ataccgtggc ggaaaacatg gcgtgccatg ccggaatcac gcgtacaaaa aagctgattg
     7921 ccatggcatg agccaccaga ataaacagca gggagacgca catacgccgc ccggccttcc
     7981 cccgccaggc gtttatccct gcttccacag tcagcaggat cagagcggca gcggcaacag
     8041 ccagcaaaat tttaggaatc ggcatgaatt cataaaaggc gtttaccaat tccggaatgg
     8101 cgctgaaacg ctccccccag gaatggagaa cggctgcgcc gcccgccgtc tccgaatgaa
     8161 ggcgggccgc attgcccgga gccagcagaa gaaaggcccc tcccagcgca tggaatcccc
     8221 agccaatgaa aaaccagcgg gggagaacca ttccccggca gcgggcgtaa caccagtaca
     8281 cgaacagcat gcccagggaa gcgggaatct ggttctcatt cgtcatgccg gcggcaaaag
     8341 ccatgacgcc tatccagagc caggaaaaag gagtgtccct aatccgggaa gttccagccc
     8401 ataaggagcg gtacaggcac aggaaaccca gccagatggc ggccgaccag gcgtaattgg
     8461 tccctccgga caaccagaac atcgtgatcc ccggcctgga tgaagacgta agcaaaagaa
     8521 gcgctcccac ggtaaacagc agcacatccc gccagtttcc ggaattgacc cttcttcccc
     8581 gcgccaggaa aaacatcatc aacgccaggg aaagaagcac gaagggattg agggccaaaa
     8641 aaagccattt tcccgccgta gctgtaaaaa tagcgaggaa ctctcccagc cggggattcc
     8701 agtgcatgta tgaccaccag caccgctcca gcacgggctg aaaagcaagg cggtggtccg
     8761 ccccgaacac ggaatgcata aagctgtccc cagtgaaagg agtccaccag gtaagcagca
     8821 tggcatacac aagaaacgcc agcaccaccg cccggaacag gaaacgcgcc gccagtgact
     8881 gcttcatgga acaggccggg ataaaaaacg ccgtccgcaa ctaatccgcg acggccttga
     8941 ataccggggt gggcagcttg tccggcttct tgccaaagaa atcccccaca tccttgaatg
     9001 tcacaaacag gaagaacccc atcagcagga acacaaagcc gaactggacg aattccagaa
     9061 cttttacatt cagaggacgc cggaaaacca attcaatggc attcatgacc acatggccgc
     9121 catccactac cggaagaggc agaatattca gaaccgccag gtttacgttc aacaccacgg
     9181 aaaaccatag agccagcttc cacccttcct ccagagagaa catcttgtaa aactgattgg
     9241 caattcccac aggtccggaa agatgctcca cgcccacgct gctgccggga gccaccacct
     9301 tcgccagggt atcccccatc catttcagac tctgcttgat ctgcgcccac ggagaaggat
     9361 ggacggtaac gtcacggccc acggaactca ggtcccagct cacgcccaga ataggctggg
     9421 cccccttgag cagggagccg tccgcaccat tgtgccaatt ctccggaagc ttcccctgaa
     9481 cattcacctg ccttgcaacc ccggccctgt ccgtcacatc cagcaagagc ggttcattct
     9541 tcttcagcag aacatccagt gcggcagggt tccagaggcg ttctccattg gcggccgtaa
     9601 cctcatcgcc cggattcaat ccggccaggg cggcaggaga attggggatc acttccccaa
     9661 tcacgcaggg catggcctgc atcaacccca cctgacgcat gccggagcgc tgccaccagg
     9721 acgtttccgg aatccggaat ccgcaggaaa tttccatctc tccttcatgt ccaggtctct
     9781 gcaccgtaaa aaccacccgg tcatgctcgc ccagcatgat caattcccgc acgccttcca
     9841 tattgcctgc ccacttgtcc acaggatggc cgtccacttt cacaatcttg tcgccgggaa
     9901 gaatgccggc ctgagccgca gggctgtccg gagcaacaaa accgaccgta gtcacgccca
     9961 tctccacatc cggcttgccc accatccaga cggccacggc aaacaacaca gccagcagaa
    10021 aagaagcggc aggcccggcg gcggccacga taatcttgtc caagggagta accggcttca
    10081 aatccttggg aagctccgca cgtccttcaa tggcctccat aggagccatc tgcggcaggg
    10141 atacaaaacc tccggccgga atccacccca gcccccactg cacgccgtta accgtttttt
    10201 tccagatggg acggccaaac cagatttgaa acctgtccac atacagacca cgccagcggg
    10261 cagcgaaaaa atgaccaagt tcatgaataa aaatcatgaa gttaaacaac atcaccacgc
    10321 cgaaaataat ggcgatagtg gcaaggacgg aaagaatgct atccatggta gtttttgccg
    10381 tccaagctac cacgggcctc tccggtgaac aagcttcaaa gatgtcctaa tgacaaagta
    10441 gatgctgata ttccaaatga atttcaaaag gtgcctgaca gcccagaaaa cagcctttac
    10501 cttccgggaa ttctgaagcc gcaagtaaaa gaccgcgtcc ccttttttcg agcggtgcct
    10561 ctgggagagg aggcatcatc ccggcccgga acctggacga ataaaagcgc cttttttacc
    10621 aggagaaaaa ccggcggggc atcaaacgcc gcagcccgct tttctgtccg aatcccctga
    10681 acagcctttg caataaatgc ctccggaatt ggaagcaatg attaaagagc cattgtgttc
    10741 ttaacaaaaa tgcgcactgg cggaaacagg atttttccca gccggcatgg gaaaaccgac
    10801 gctgcaataa gagaaactgg ctggcgcatt aaatgatgaa cgtgcgaaca cctgctccat
    10861 aaagcattta caggaaaata ccaacggccg gaactgccat atccggacat gaaaacagcc
    10921 tccggaaaat tatttccgga ggctgtcggt taacgtttac tgaacagatg gaaactcccg
    10981 cacattcagg cgcggagagc ggcgccatcc ttggcttcaa tggccttctt cgcttgagcg
    11041 aagatagcgt caaaagcctt ttcgtcagag acggccaaat cggcaagaac cttgcggtcc
    11101 aaatcgatat tggcggcttt caagccttcc ataaagcggg aataggtcag accgcggtcg
    11161 cggacggcag cggaaatacg ggcgatccaa agacggcgga attgtccctt ccgacgcttg
    11221 cggtcgcggt attcatactg ccaagccttg taaacagcat ccttggcgta gcggaagagt
    11281 ttgctgcgga aaccgcggaa acccttggca cgcaagagaa tgcgcttacg acgcttgcga
    11341 gaggccggtc cattagtggc acgtggcata gcttatttct atttctatct tgaacagact
    11401 gttctnnnnn nnnnntcggc attagtcgag cgtttccgcg gtttaggcga aaggcaggtt
    11461 ctgcttgact gcccagactt gcgtctcgtc caccagagcc accttgccaa gctggcgctt
    11521 gcgcttgctg gacttcttct ggaggatgtg gcgcttgccc tgtttacggc gcagtacctt
    11581 gcccgtgccg gttaccttga aacgcttggc gactgccttg cgggttttat tgataccacc
    11641 tttgcgggtc atggtggaag gagattacag gctttttcca aatacgcaag tattttttat
    11701 gcaaatcttt tcttcagacg atggcaaatg ggcccctcct gaccatcatt gcccggcgcc
    11761 tttgacgcag ccggctgcat caagaaataa aatggggaac ataaccggca aaggatgatc
    11821 agacagctac tgctgccagc ctgggaatcc tctgtcatcc gccataccct ttgccacttc
    11881 ggcaagtgct tatcatgaga aatcttatat ataagaaaag actggaacaa agagctcttc
    11941 atatactccc ccatcggcgt aaggccttct tagaatattt cacgttgaca gcagactccg
    12001 gtccggaaca ctgacgtcac atcagcagaa ttctttaagc aattaactgt taaacccaat
    12061 atccaaatca ccgtcaaatg agatcaagaa taagatggac aaatatcacg gaaaaacagc
    12121 atgccatcct accagaaagc tcatccccaa ttcatcacgc cggatattat cccctgcctt
    12181 ttccatattc tccgcgcatt tgcctgattt catccctaat caaggcggcg cgttcaaatt
    12241 ccaaatggga agcagcctcc ttcatttccg cctccagcct ggcaatgcgc tcttccagac
    12301 catacccggc ttcatcctcc gctacgnnnn nnnnnncaat accgtgttcc cggttgtatt
    12361 tttcctgaat accccgacgg taatccgtca gctcaaccag ccgtttgatg gaatccgtca
    12421 tcacatccgc aaacaagacg cactctccat tcaaatgccg cgcggcgcgg cccgccgtct
    12481 gcaccaggga tgtctcattg cgcaaaaacc cctccttgtc cgcatccaga atgcacacca
    12541 gggaaacctc cggcaaatcc agcccttccc taagcaggtt aatgcctacc agaacgtcta
    12601 tcttcctggc tctcaaagca cgtaaaatct ccacacgctc aatcgcatcc acatccgcgt
    12661 gaatgtagga caccttcaat cctacccctt tcaggtactc cgtcaaatct tccgccgtct
    12721 tcttggtcag cgtcgtgacc agcacgcgtt catttttggc aacgcgttca ttgcacatgg
    12781 cgatcgtctc gtcaatctgc cctttcaatg gacggatggt aatcttcggc tccagcagcc
    12841 cggtaggccg gataatctgc tctacaatca ggggtgtaga acgcctggtt gggtcaaatt
    12901 tttctaccgg ttccgtggat gggctgggag caacgcgaat gtccttggga gaggtaaaca
    12961 aaattccttt gaacccttcc ggagcctttt ctccggatct ggcggcacgg cgaacgggaa
    13021 tatacgtctt attatccgca cggcaattta ccagttcaaa tggccccgga gtagcggaag
    13081 catacacaat ctggttctgc ctttccataa actcatggaa tttcaaaggc ctgttatcca
    13141 gagcgctggg caggcggaac ccatgctcca ccagaatatt tttgcggctt cggtccccct
    13201 cgtacatgcc tcccagctgc ggcaccgcca catgactttc atcaatcagg gtcaatgaat
    13261 ccttgggaaa gaaatccagc agggtggatg gggcgcttcc gggcaggcgg ccggacaaat
    13321 ggcgggaata attttcaatc cccttgcaga aacccagctc ttcaatcagt tccaaatcat
    13381 aatccgtgcg caatttaagc cgctgcgctt ccagcaggcg cccatgcttc tcaaaccagc
    13441 ctacgcggtc ctccagct
//
LOCUS       DAWEMX010000064        13380 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1948_length_13380_cov_15.442251, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000064 DAWEMX010000000
VERSION     DAWEMX010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_13380_cov_15.442251"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3182 a         4087 c         3480 g         2631 t
ORIGIN      
        1 gggcctgacg cagggattcg tctcccggca gggaaccgag caccagcacg gaacagtccg
       61 gggtgacgga aggagggaag gagcattttt tcacggcggg aaacggtcag ggggtctaca
      121 tgccggaatg cctgcgcact tccttcatgc ccacttcctg ccggagcagg gaggacagcc
      181 acgggtggct tcccacggag ggggccaggt tgaaccgttc cggcctggtg atgcgcgtga
      241 aaatgacgcg cttgatccgg gatttgggca ccgtcctggc tccgtaaccg tccggaatgt
      301 tggaggacca gaacgttacc tggtcccctc catccttcac caggacggtc agatggccgg
      361 attctcggcg cccgatgcgg tccgtccaga aaattttcat gaagtcgccg gggcgggcct
      421 tcctccagtc ctcaaagttg atgccggctc ccagcctgtg caccagaacg gccaagccgg
      481 ggccattggc gttcgcccat ccccacggcc cctccccgtc atgctggcca aagcggggga
      541 tgagggccag ccacgcgcgt tcggaaataa cgggccgcgg ctgtgcagag tcccatatct
      601 gcagggattt caaaaggagc aggtagcagg cagaggaaca gaaggagggc gtggcgttac
      661 ggagaagaaa aacgggacgg cgcgtgcggg ggtcccagcg gcaggctttc tccgcaaggg
      721 cctgcatggc ttccctgctg gtttcgtagc cgccgccgtt ccggaactgg gaaagtccgg
      781 tccgcagggc ctggtaccag gaagccgcct ccgaaggcac ggcgctcccc cgccccgtgg
      841 aagaaaggtc cgcagccgat gctgcggagc acagcaatcc aagacagcaa attaatgtca
      901 gggcgttcct catccttgct ccgcagggta tacggacggt ggaaaatatc aagggcggat
      961 tgcgtgcggc actgtacgaa aaggaaagaa ggccatagcc ttttccccgg aattcctgtt
     1021 gttttgtctg gacttatcgc ccgacgtccc gtactgtgcg tcaggtcacg cgccgggtat
     1081 gcggagcgga gaggcaacgc accttgggcg gaccttgttt ccggcattta tttttaacca
     1141 ccacggacca catagaggca tcataccatg gcaaaacgcg aagatatccg caaaatcctc
     1201 attatcggct caggccccat catcatcggc caggcctgcg agttcgacta ctccggcacc
     1261 caggcctgca aggccctccg cgccgaaggt tacgacgtcg ttctggtgaa ttccaacccc
     1321 gccaccatca tgacggaccc cggcatggcg gaccacacct acattgagcc tctgactgtg
     1381 gaccgcatca cggccgtgat tgagaaggaa cgcccggatg ccctgctgcc caacctgggc
     1441 ggccagacgg ccctgaacct ggcttccaag ctgcatgaag aaggcatcct gaaacagtac
     1501 ggagtggaag taatcggcgt ggatctggaa gccattgccc gcggcgaaga ccgcatcatt
     1561 ttcaaggaaa ccatggacag gatcggcgtg ccggtcgcca agtccgaacc cgtctactcc
     1621 gtggaggaag ctgaaaaggt ggccgctgaa ctgggctatc ccgtcgtgct gcgccccgcc
     1681 tacaccatgg gcggcacggg cggcggcatc gtttacaacg tggaagaact gcgccaggta
     1741 gtcccccgcg gcctggccgc ctccctcatc aaccaggtgc tggtggaaga atgtgtgctg
     1801 ggatgggagg aactggaact ggaagtcgtc cgcgacgcca agggacaaaa gattaccgtt
     1861 tgctttattg agaacgtgga tcccatgggc gtgcacaccg gagacagctt ctgcgtggct
     1921 cccatgctga ccgttccgca ggaagtgcag gataaacttc aggattattc ctaccgcatt
     1981 ctggacgcca tcggcgttac gggaggcaca aacgtgcagt tcgcccacaa ccgggaggac
     2041 aaccgcatca tcgttattga aatcaacccc cgtacatccc gttcctccgc cctggcttcc
     2101 aaggccaccg gcttccctat cgcctccgtc tccaccaagc tggcctccgg cgtcacgatg
     2161 gacgaacttc cctactggcg caaaggctcc ctggaaaaat acgaacctta cggcgactat
     2221 gtggtcgtga agttcgcacg ctgggccttt gaaaaattcc ccaaggcagc cgacaagctg
     2281 ggcacccaga tgaaagccgt gggagaagta atgagcatcg gacagaattt caaggaagct
     2341 ttccagaagg ccgtgcgctc tctggaaatc ggacgcgccg gcctgggccg cgtgaaaaag
     2401 ctggaagccc tctccacccc ggaattgcgt gaaaaaatca cgtacggcaa cagcgaacgc
     2461 ttcttccaga tttacgaact cctgcgccgc ggcgtagccg tggaggaaat cgtgaagctg
     2521 acccgcatca ctccgtattt cattgaacag atgaaggaac tggcggactt tgacttttcc
     2581 ctggatgggc aggactggga ttccctttcc gcggaaaccc tgcgccaggc caaggaaatg
     2641 ggtttttccg acaaatacct ggcccagatt ttcggggtgc cggaaaaggc cgtccgcgcc
     2701 cgccggcaga aagacggcat cacggtcacc ttccgcatcg tgcccgtcag cggcgtggaa
     2761 cacgcggaat actactactc cacttacagc ggcgcggcgg acgaagttcc cgtgaacgat
     2821 aagaagaaaa tcatgattct gggcggcggt cccaaccgca tcggccaggg catcgaattc
     2881 gactatacct gcgtgcatgc cgccttcacc ctgcgtgacc acgggtatga atccatcatg
     2941 gtgaactgca acccggaaac ggtttctacg gactttgaca cggccaacaa gctgtacttt
     3001 gaacccgtga ctgtagaaga cgtgctggct atttacgaga aggaaaagcc ggaaggcgtc
     3061 atcgtccagt tcggaggcca gaccccgctt aacatcgccc aggccctcaa agacgccggc
     3121 gtgaaaatca tgggaaccca gccggaaggc atccgtctgg cggaagaccg cgaatatttc
     3181 cgtgaacgca tgatcgccct gaatatccgc cagccggaaa gcggcacggc ccgttccctg
     3241 gaggaagctg tggaactggg gcgccgcatt ggctacccgg tcatggtgcg cccctccttc
     3301 gtgctgggcg gccgcggcat ggaagtcatt tacgatgaag aaaacctgaa gcgctacggc
     3361 gtggaggcca tccaggtcag cccggaatat cccatgctga tcgatcgttt tctggacaac
     3421 gccattgagg cggaagtgga cgctctggcc gatggcacgg atacttttgt agccacggtc
     3481 atggaacaca ttgagctggc cggcatccat tccggcgact ccgcctgcgc catccctccc
     3541 gtgacaattg ctcccaagca catccgcacc attgaggaac atgccgccaa aattgcccag
     3601 gacttccagg taaagggcat tctgaacgtg cagttcgcca tctgcaacga tgaggtttac
     3661 atcattgagg ccaatccccg cgcctcccgt acggtgccga tcgtctccaa ggtaacgggc
     3721 atctccctgg cgcgctacgc cacggaaatc atgctgggca agaaactcaa ggagctgggc
     3781 ctgaagtcgc gcgcctgccg tttcatcggc gtgaaggaag ctgtcttccc cttcaacatg
     3841 ttcccggaag tggatcccgt actgggaccg gaaatgcgcg ccacgggtga agtcatgggc
     3901 attgcggaca acttcggcat ggcctactac aaagcccagg aagctgcggg ctgcattctg
     3961 cctaccgccg gcaaggtgct cgtcacggtt tccgaccggg ataagaagtt catcgaaccc
     4021 atcgcccggg acctgatttc cctgggcttt acgattgtct ccaccggcgg caccgcagaa
     4081 tacctgcgcg gccagggcgt ggaaacggaa gtagtcaaca agctgcatga aggccgcccg
     4141 aacctgggcg acatgatcac gaacaagcag atcgacctga ttatcaacac tccggtggac
     4201 cgcaccagta tgattgacga ctccttcatc cgcatgcagt ccatccagaa gaaaattccg
     4261 tacatgacta ccatcgcagc cgccagagcc acggtggagg gtattcgctc cgcccagcac
     4321 gtgaaggtat caccccgttc cctccaggaa taccattcct gaacgggaaa tactccggct
     4381 atttcaataa aaccccgcat gcctgaaacg catgcggggt tttattcatc gtcgggcaga
     4441 cggaggatgt gaatctcaaa aatcgcccgt ccgcagatta cctccttgcc gcactaatgc
     4501 agatcaagcc cttgctatct ctgcgcaaat caatgtggca ttatcctggt tgggcgcgcc
     4561 gtcgcggtta atgccgtcca gaacggaacg caccagttcc tccgcggaaa gcatggacaa
     4621 ataccgcatc cctgccggcc ccagggaatc cagataaggc cccactccgt cactggcgac
     4681 caaaacacgg tcccccggaa ataagggaaa gcattcctcc cgcaaatcca ccaaaggaac
     4741 ttccttcccc atcagggcag aagtcaggga atggcgggca ggatggctca aagcctcctc
     4801 ccggctgatg atcccttcat ccgccatgcg ctccaattcc gcggaatact ggtgcagctc
     4861 attaagcaga aacattttct cttcccggat aagcagcaga agggagtccc ccacgctgac
     4921 ccacctcagt ccatccgccc ctatccaggc tgctaccagc gtcgtcccca tctcgtcatc
     4981 ctcccctctt ttcttccggg cggaccatac ctgttcattt gcggcagtca ctgcagcttt
     5041 cattctctct ccgggcaaac cggatttccg ctcaaaagcc cgcgaacagc cttccaccgc
     5101 cgcgcggctg gccaactctc cgccggtata cccacccatg ccgtccgcca gcaccagtac
     5161 caatgcgtcc tctccagcca gaatggagaa agaatcctgt tggttactgc gggcgcccag
     5221 aatctggtcc ccatgcaatc cggaaaccag aaaaggctct tgggaaaaag tctgatgcat
     5281 catggcagta aataatcgta acatggatgg cctgccttaa taaagcaaga atagcgagag
     5341 aaaatagcca tgcctgaagc cgcggaatga ttatttccag aggaatggaa acgtcagcct
     5401 gcatggctgg aaaaacgcga agggaacgcc ccggcagaaa gaacccatgc aaaaccggaa
     5461 tgataagcgg aaaaacagcc attgaatgcc ccaacccgca agcgctccca gcgctctccg
     5521 gagtgttgcg ccagggaaca ctttccgccc aggaagaaga aaggaaagtt tttactggcg
     5581 gcaggaaaaa taattctcta ctatattttc tgatttttta ttccatgagc cttcattccg
     5641 cccccctcca tcgtttggga actttccgga gagccctgtg aaacgcggga tcaagccctg
     5701 tccggcatta aaaataacac ggaagctttc attatctgca gccgcggtga cgaattcctc
     5761 caggccacct gcaatagtcc ggaaaattac cattgcgaaa tttcgtttgc cggcggaaag
     5821 gacagtgagc tttacatgac gcccaaagac ctggactact ctcggctcca tgaccttttt
     5881 tcacgctatg cggcagggga ggatctttcc catctcaaaa aggagtggac tcttgatgcc
     5941 cacaaacccg gctatattac cgccatcgtt gtcgttctgg caatcattgc cgtccttgcc
     6001 gtcatcatct tcagttcctg aaccgaaaag aggcctttcc ttttccggag gccatgcatg
     6061 aacgccgcat cctcagcact tccctagata caccacagtt tcattcggga tcgtaacgcg
     6121 tccttccgtt tcaaaccggt caaacagctt ttccagggcg tccagccagg aggatcgctc
     6181 ttctccgccc tccgtcaagg cataggaggt ggacatcatg cggcggagat actgctcccg
     6241 cgtgtaggac aaatcatttg gaaagcggaa tatctggaaa cggtggttga aaaacgctcc
     6301 aatgctgtcc gtcaacttcg ccagccctcc gctgaatcca taaaaacacg gacagtaaag
     6361 acggtggata tttccttcct cccgggcaat gggactgtct tccaccctgg agttccaaat
     6421 cagggcaacc ttgcctcctc cggacagaat acggcggcat tccctcttga acgcggcggc
     6481 gtcaaaccag tggaatgact gcgccgccgt caccagatcc accgaagctc cgggaagccc
     6541 ggtccgctcc gccgttcccg ccacggaatg aaaacgggga aaagcggaca gccgtttctc
     6601 cgcttccttc cgcatgtcat cattgggctc cacgccatac acggtccagc cgcgctccag
     6661 catggcgcgg gaaagaatgc ccgttccgga acctatatcc gccagacggg gattctcccg
     6721 gcggctctcc cgcgtcagaa gcccaacaac ggaggcggga taatcgggtc tgccctgggc
     6781 ataagcccgg gcttttccag taaatcggtc agtattattc attagttttc agggtatggt
     6841 ttactccttt tctcccgctg ttccagcctc aatgccgtac ccggctcaag aaaagccgcc
     6901 atgctgcggg agcccgggaa aaaacggatt tgcccggtga ccgtcatgca gaaaggcatg
     6961 cgggaaagat tgcgttcctt gcccgttccc cggcaacatg gacggagcag tccctacaga
     7021 tggctttaca agtccttcat gaacttttag tatccggctc aggcccgcct ttcccggatt
     7081 gtgtcctgac tacgggaaaa acgaacatct gcgggttcca tttttcagac tgcgccgccc
     7141 atgttcggat attacagact tgcctccgcc gtcccccagc tccgcgtggc tgaagtggac
     7201 tacaatgtcg accaacttac ggagggattc cggaaagccg cagaacagca ggcggcagcc
     7261 gtcgttttcc cggaactgtg cattacgggc tattcctgcg gagacctttt cttccagccg
     7321 aacttgagga aagcagccct gaacggcctg ctccgcttta cggaagccac ggaaggctcc
     7381 ggcacgattg ccgtggtggg cctgcccttc cttcatgaag acgctctgta taatacggcg
     7441 gcggtagtgc agtccggccg cattctggct ctggtgccca aaaccgtgct ccccaactac
     7501 cgggaattct acgaaaagcg gcaattcacc tccggcaggg aattgggtac aggagtgaag
     7561 gaagtcacaa tcaatggggt gcatatcccg ttcggggagg aaatcgtttt tcatgaagaa
     7621 tcgtctccct tcagttttgg cgtggaaata tgcgaagacc tgtggagcgt cattcctccc
     7681 agttccaaac tggctcttct gggtgccaga gccattctca acccttccgc aggaacggaa
     7741 ctcaccggta aggccgcata caggagggaa ctggtcagac agcagagcgg cagatgcctc
     7801 tgcgcctatg tcctttcctc cgcaggtgtg catgaatcca ccacggacac cgtattcggc
     7861 ggccattccc tcattgcgga caacggcagg ccagcagcgg agggggaacg gttctgcagg
     7921 ggaagcaccc tcatctttgc ggacgtggat tttgaacggc tggaggcggc gcgcctctct
     7981 gaaagctcct tcaatgatag caaattcttt ttccctgccg ggaatgccct gcatctggcc
     8041 ctgccggaac aggttcccgg agcgcccggc ctagaatacg cattcaaccc ggcacggccc
     8101 tttctgcctt ctccgtcccg ccgccgggaa cggtgtgagg aaatcatctc catccaaacg
     8161 gcaggtctgg ccaaaagaat ggaacatacc cgcgcgcaac ggctcgtcat cggcatctcc
     8221 ggcgggctgg actccacgct ggctctgctc atctgctccc gcgcctgccg tgcgctgaag
     8281 cgccctgcct cggacattct ggccgtgaca atgcccggtt tcggcaccac ggacagaacc
     8341 catgacaatg ccgtaaccat gtgccgcctt ctgggcgtgg aacttcgtga aatccccatc
     8401 tcggaatgct gcctccagca cttcgccgac atcgggcacg atcctgccga acgaaccacc
     8461 acttatgaaa acgtccaggc ccgcgaacgc acgcaaattc tgatggattt ggccaataaa
     8521 accggggggc tggtcgtggg aacaggggac ttgtcggaaa ttgccctggg atggagcacc
     8581 tacaatgggg accacatgtc catgtacgcg gtcaactgct ccattccaaa aacgctgata
     8641 cgctgcctca ttgaacacat cgcggaagaa tcatccccgg aactggcagc caccctcacg
     8701 gacatcaaca acactcccgt cagcccggaa ctcctgccgc cgtcggacga cggcaccatt
     8761 gaacagaaaa cggaagatgt gcttgggcct tacgacctgc acgacttttt cctcttccac
     8821 ttcattaaat acggtgcgga accggataaa atcctgcacc tggcggaaca tgccttccgc
     8881 ggcgaatttc agccggactt catccgccgc tgcctcggca tcttcatccg ccgtttcttc
     8941 cgtcagcaat tcaagcgcag ctgcatgccg gacggcccca aagtgggcac catttctctt
     9001 tccccgcgcg gggactggcg catgccttcc gacgcctgcg ggaccgtgtg ggaaaaagcc
     9061 atctcccact actgagacct cctatccgga aagttacgga gacggctttt cccgaaaccc
     9121 ggcaaggatg ccgaagaccg catcaatgcc cgggggccaa cttcagcccg ataatgccta
     9181 tcagcaggaa ggatgccgcc agcatgcgcc agatattgga agaatccccg aaccacagaa
     9241 taccaaccag gaacgtgccc aacgcgccaa tgccggtcca taccgcataa gcggtgccga
     9301 tggggatgtt ccgctgggca agccacaaaa gaaaaccgct cagaaccatg ctgaaaacgg
     9361 agcaggcgat acaggcggcg aatttgccgg gccctgtgcc gggcgtttga gaaagtttga
     9421 aacccagagg ccatccgatc tcgcagagac cggccaacac cagataaatc caggacatat
     9481 attgaaacgg ggaatcacct tccgctcacc ggccttccca aatgggaaaa gctccaaggg
     9541 caacggatgc cttgcagaat atccgcaagc gttctggaat caagaaggta aatcttccac
     9601 accctgtcat acagaacaac acaggcatcc gggtcggaat cccgcctctg tcctgtacaa
     9661 attccatcgt ggtacctgtt acaggcaatc tgacaaacgg aaaaccgcta cttcatgaag
     9721 aaagctcttc taatgacaaa ataatattgt ttgacgcttc tgtttacatg ctccccgcca
     9781 tctgccttta ccccggcctg ccggcccggc atctccgtat tcagggataa tcacggacca
     9841 gcatcaaaac cggaaccgtt acgacaacgg cgcttatcac aaacaaaaaa ggagcgggaa
     9901 aagctgtttc ccaaagtccc tttctattta agtttcctgc cgggagccag ccctgtgtct
     9961 tctccgtccg tcagccattc catggtaatg ggaacaaact tcagcttttt aatatgctcc
    10021 tggttctcct cataaagcac cccaacgatg ccgggttcca ccatggcaag gctggaatag
    10081 gcaaaaccgc cctcgcccag caatttcttc accggccagg tcttgccgtt gtcatagctc
    10141 atggccacct gtcccttaat gcgccggccc gcactaggcc cggaaaacag aatgcggctt
    10201 ttgccgccat aggcaggctg gtcgctcagg gaatacgtca tcagggaatt ctgggtactg
    10261 tcgcaaaaca gctccggagc gtcctccacc tgtccccagg tctccccgcc atcctgggac
    10321 cacgcaatac ggcggcaatt gcctgcgccc caatggcgcg caaccatcac aacgccgccg
    10381 ttatccgttt ccgcaatgga cgtctcgttc accatgccct tcatattggc agtcggctgg
    10441 cccagcttcc agctcttgcc gccgtcatcg ctgtaaatgc aggaaatcac ccatttgccg
    10501 aacggccctt cattcatcgg aatcaccagg cggcccttgt gggcgccgct cttcagctgg
    10561 gttcccgcat tcgggccgga cgccataata tccactccgg aaggacgctt ggtcgtcttc
    10621 gtgatctcct gcggcttcgt ccaggaagaa cctccgttcc tgctctgaat catgaaattg
    10681 cggatgcact tttcatcatc ccatccgtcc gggatattgg gctgccgctc cttgaccccg
    10741 gcagggtaac gctggaatac gacagtcacg gtcctggttt tggcgtcata aacggggcac
    10801 ggattattaa acgtggcgcc atgggccttg gcaatcgccc ggggacggga ccaggtcttc
    10861 ccgccattct tgctgacgct catgataata tcgttctccc cctggtcggt atttttgtac
    10921 cgtccttcgg cgaatgccag gagctggccc ttgccgatac tgagcagggc gggaatacgg
    10981 atggacgcat agggattacc ctccccggcg ctgaatacag tcacagccct gtccgtaggg
    11041 aaaccggttt tctcttcctg cccgaacgcc ggcaatgcca cggcgcatac ggcactcatc
    11101 aaacacttaa atacggaatt catcatcctc ttcaccttat tccattttcc cctgccgcac
    11161 aacaaccatg tcatgcatgt gcgccaagac cagagaaaaa ggaacactaa agaggaacgc
    11221 gcggcaggaa cgcgataccg ccgccgggca gcccggaagg cttcggccca aaattcaaac
    11281 cagcaagaaa cgtgcccatc cgaatacgga tccccggaaa agcgcccgcc ggaaccgggg
    11341 aaaatatccg gaaacgcgaa atcgtctgaa aaatccctgc cgcaccgcca aagccattcc
    11401 acacatcaac cgcccggcct ctcttctaac gaactacctg cctatttatt aatcaagaga
    11461 aaaatagtga aagaagaaga aaacggaacc tgtatcttgt aacatcacag aatcacctgc
    11521 ttcccgtact gtcatgaacc gaatccttcc attactatcc cttcccctgc tgtcgctcgc
    11581 agcggtattt gccgctaata cgccggaaca catcggcaac gacctcaaac tgttcaaaga
    11641 ctcctcctgc acctccctga agccggatgt gaaagacacc tccgccttcc agtctgacgc
    11701 gatgaaggaa ctcgcgacca aaatcctggc aggccattac aagccggact acctgtatgc
    11761 cgaataccgg gccctcccct caccgcgcca gacggggaaa aatctcagaa tcggcgacgg
    11821 attcagcaaa tacgacaaca tgacgggcgt ctatctggaa aaggggaggc atgtcgtcct
    11881 ggtcggcaag acggaggggc aggaaatcag cctccttctg ccgaacctga tgcgcaagcc
    11941 ggccgaagga gtgcagccca caaaagaccc caacggctgg ggattgcata aaaagcagat
    12001 tccgctcaag gaaggaatca acatcattga cgtggaaacg cccgccaacg cctatatcag
    12061 ctatttcacc gaagacgccg gcaaggcgcc gaaaattccc gtccacttcg taaccggcaa
    12121 agccaacggc tactttgaca ccacccgggg cgacaccaac aaggactggg ttcgcctgct
    12181 tgaccaggcc gtctctccga tcatggatgc ccggggaaaa tacatccagg ttgcctaccc
    12241 cgtagaattc ctgaaaaaat tcaccaaaga ccgcggaacc gaactcatca acgcctacga
    12301 caagctcatc ggcatccaat accagctgat gggcctggat aaatacggca aaatcccgga
    12361 aaaccgcgtc ctggcccgcg tgaacttcaa ctactacatg ttccgcgacg gagacggagt
    12421 cgcctacctc ggaaacgacg ggacaatgcg catggtgacc gaccctgaaa acgttctcaa
    12481 gggcgatgcc tgctggggat tctcccacga agtaggacac gtcatgcaaa tgcgcccgat
    12541 gacctggggc ggcatgacgg aagtcagcaa caacatcttt tccctgcagg ccgcagccaa
    12601 aacaggcaat gaaagccgcc tgaagcgcca gggcagctac gacaaggcgc gcaaggaaat
    12661 catcgaaggg gaaatcgcct acctgcaatc taaggacgtt ttcaacaagc tggttcccct
    12721 gtggcagctc catctttact ttaccaaaaa cggacacccc gacttctatc ctgacgtcat
    12781 ggagtacctg cgcaacaacg cgggaaacta cggagggaac gacaccgtca aataccagtt
    12841 cgaattcgtc aaggcatgct gtgacgtcac aaaaaccgac ctgacagact tctttgagaa
    12901 atggggattc ttcaaacccg gaaaattcca catcggcgac tatgcccagt acgactttaa
    12961 cgtcacccct gaaatggtgg aggaaacgaa aaagtggatt gccggcaaag gctacccgaa
    13021 acctgaaacc gacatcaccg aactaagcga gtaatgtgat tcggcaatcc cgacgattcc
    13081 ctttaatcat caaatatgct gcaaacggat aagaaaaaca gggactcaag ttcaattctt
    13141 ttgaaagtat ttttgatgat ggaagattat taagaagtta aactccctca ccaaagggtg
    13201 gaatctttgc tgattccacc ctttttattt tgctcgtaag atatttcccg ctatacgggg
    13261 cacagcaaac ggtacgatac ctcttaccag atcaaaaact cccaattggc aacctatctc
    13321 tgtttgatct caattctcaa atgttttaaa atgttgttct caaatgattc cgcacagtga
//
LOCUS       DAWEMX010000065        13376 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1949_length_13376_cov_10.205165, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000065 DAWEMX010000000
VERSION     DAWEMX010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_13376_cov_10.205165"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3839..3848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2977 a         3128 c         3668 g         3593 t
ORIGIN      
        1 atgaagctgt gcgcctgggc gaaggaacgc gtggagcagg ggggggcgcg catcaaaata
       61 cgcctggtga aaggcgccaa tctggcgatg gagaaggtgg aggcctccat gcatggctgg
      121 gcgcaggccc cgtatggcac gaaagcccag gtggatgcca attacaagag aatgctccat
      181 tacggctgca tgccggataa tgccagatat gtgcagttcg gcgtggcttc ccacaacctg
      241 tttgatttgt gttatgccat gctgctgcgt gagcgggaag gcgtgcgtga ccaggtggaa
      301 ttcgaaatgc tggaagggat ggcgaaccat caggcgcggg tcatccgcca ggcggcggac
      361 ggtctgctcc tttatgcccc tgtggttttg aaggaagatt ttcacagcgc tattgcctac
      421 ctggttcgga gactggatga aaataccagt gaggagaact tcctgcatga tctcttcggc
      481 atgacaccgg ggtcccggag ctgggaggtc cagaaaaaga ggtttttgaa ggcctgtcag
      541 gaaaaggatg aggtgaagta cggccccaac cgcacgcaga accgcgctgc ggatcccatc
      601 cagccctcgc attaccggga cgcctttgcc aacgagcggg atacggactg gtccctgcgg
      661 cagaatgcgg aatggatcaa cgggctgatt gccgcggaga aggaaaaatc cggcgaggaa
      721 atccctctgg ttatagatgg tgaggaaatt acgactaatc tatggggcgt ggggcgcgat
      781 ccgtcccgcc ataatgaagt ttcctataaa tttgcttatg cggattttga ccaggttgaa
      841 catgccttgg tcacggcaga cagggcacgc tcctcctggg cgtccaaaag cattggcgaa
      901 cgcgctgaaa tcctgcaccg ggcggcgcag gagctgtcca gaatcagggg ggaagccatt
      961 gctgccatgg tgagggatgc cgggaaagct cccacggagg ctgatgtaga ggtgagcgag
     1021 gccatagact tctgccgcta ttacgcggaa ggcctggacc gcgacggaat gaacgacggc
     1081 gtggaaatgt ccccgctggg taccatttgc gtgatgtctc cctggaattt ccccttcgcc
     1141 attccgacgg gcggtgtagc tgccgctctg atggcgggaa atgccgtggt gttcaaaccg
     1201 tccgaactgg ccgtttatac ggcttggcag attgtccagg cgttctggcg tgccggtgtg
     1261 cctaaaaacg tccttcaatt cgtgccgatg ccgcgcaatg aaatttccct caagtttttg
     1321 atggatcccc gattgaacgg cgtgatcatg acgggatcct accgcaccgg aaaaatgctg
     1381 cgcgaactgc ggcctgacct gcatgttctg gctgagacca gcggaaagga tgccatgatc
     1441 atcactgcta cggctgatcc ggaccaggct gtaaaggatt tggtgaaaag cgctttcggt
     1501 cattccggac agaagtgttc cgccgccagc gtggctattg tggaggcttc cgtttatgac
     1561 aatcccgcct ttttgcgtca gttgaaggat gccgccgcca gcctgaaggt aggcgggtcc
     1621 tgggaggtca actccgtggt gacgccgctc atcagggagc cggaagggaa tctgctccgt
     1681 gcgctgacgc agctggaacc cggggaggaa tggctgctca agccggaacc ttcggaagac
     1741 aacccgtgcc tctggtctcc cggcatccgg ttgggggtga aaccgggaag ctggttccat
     1801 cagacggaat gcttcggtcc ggtattggga atcatccgtg cggaaaacct ggaggaagcc
     1861 attgacatcc aaaacgactc cgaatttggc cttaccggcg gccttcagtc cctggatgaa
     1921 cgggaaattg ccttgtggaa aactaaagtg caggtgggca acgcgtacat caaccgtgtc
     1981 atcaccggcg ccattgtccg ccgccagccg ttcggcgggt ggaaccattc ctccatgggg
     2041 cctggagcca aggccggagg tcccaactac cttaccatgc tgggaagttg ggaggaaaag
     2101 gcgctgcccc aaaagctgcg cacgccgggt gaacgtatct ccggactggt ggaaaaactg
     2161 tgttccgagc tgccggactg cgccaagcgc atccgttccg cagctggttc ccaggccaag
     2221 tggtggatgg aggaattcgg cgtggatcat gatccctccc gtgtttacgg agaaaataat
     2281 accttccgct atatcccggt gaagggaatt ctggcccgtg tggaaaacat gtccgacgat
     2341 gacgtcgcca ttctgctgct gggggcgaaa ctgtgcgggg tactcctgca cctgagcata
     2401 gggacgagcc gcccctggat tcagaaaatg catggttatt atgcttccct gacggtggaa
     2461 acggaagcgg aattgatcgg acggatgccg gaagcccttc caggcatacg gttcctgcgt
     2521 ggaacagata tttccgagac tctggccaat gccgcccgtg cccgggatgt ggaagtactg
     2581 gaccgtcccg tgcttgccaa tggacggttg gaactgctgg ggtatttccg ggaacagtct
     2641 gtttccgaaa ccgttcaccg ctacggcaat ctcatcccgc caccaggcag ttttaaaaca
     2701 gacagcgtgt gaagggtagg gaaggcctga atcccggctt ccgggtatct tagccataaa
     2761 aatgaaggga aatcctcctg ccggtccaaa aggaaaacat gggtgttgag tgtgttcccc
     2821 ttccgtttcg gaatagctct tcactgaaag gaggaaaaaa atttttcttt gcaaggaggg
     2881 aagaggagta tcctgttgaa tgaaatatga acttcgccga atgagaaaac tttcaccagg
     2941 catcacggtg aacaattccg gtggtgcggg aaaagtttcc atgaattctt atggggaaga
     3001 ggcgtggaag cctgcaggaa gcgcaggctt tcccgaagag ctttcccctg ttgccgggag
     3061 ggagggattg ggtgcttttt gtttcttttc tccatttgat gatagaacgt ttccgggaaa
     3121 cgggtatatt accaggaacg tttgcgatga cgcggcgctt tttttcctac gtaaccatga
     3181 acaccaacca atcattgaac tcccgcggca gccacatcgc ctgggtggat tttctgcgta
     3241 tcctggcctg tttcctcgtt gtcctggccc attgctgtga tccgttcgtt ggaagttttg
     3301 acggttcctt caactttaag tccgctgtct tctggggaag tctggtacgg ccgtgcgtgc
     3361 ctttgtttgc catgatctcc ggagtgttgc tctttcccgt caccttggaa atgggcgctt
     3421 tttactccaa gcgcctcaag agggtgctgg ttccgttcat tgtctggtca ctggcgcttc
     3481 ccttgctcta cttcggatac tttgccgcag gcgttcaaac ggccagcccc aacatcgtga
     3541 tggacactta tacctggagc gccaccgtcg gcaagctgta taccttcttc ttcaacttca
     3601 actatgatac cacgcccctg tggtatgtat acatgctggt gggcctgtac ctcttcatgc
     3661 ccatcatgag cgcatggctg acgcaagcca ggaggaaaga tgtgaaaatc ttcctgggta
     3721 tctggatatt cagcatgact ctcccctaca tccagatgct tgccccggca ctgggttatg
     3781 agggcaatta cggcaacatg ggtattctgg gtgtttgcga ttggaatccg tacggtatnn
     3841 nnnnnnnnaa atacccgctg gcctggagct ggaaaaaaac gctgtccatt actattcccc
     3901 tctttttgat tggttttgcc gttacattct tcggctttct ggaaacgcag aagcacttcc
     3961 ccggccagta ttccaagctg gaagtgctct ggtatttctc cggaatcaat gtattcctga
     4021 tgacctttgc catctttgcc gtcgtcagcc ggctcagaat caaggctggt ccagtgctga
     4081 gcaaggtggc ggcgcttact ttcggcgtgt atctgtgcca cttcttcttt gtccagtgct
     4141 cctatgactt cgtgaacttc atcgggctgg gagggctgcc ctccgccgtg agaattccgt
     4201 tgatggcctg tctggcctcc gctgtctccg cggcgttggt atggctgttg agcctgaaca
     4261 ggtggacgcg caaaagcatc atgtaaaccg cggatatttt ccgcagggcg cttcgggacc
     4321 acggctatcc cggggtgcct cttcttttgt ggaaaacaga ataaaacggt ttccggggag
     4381 agtcctacaa aattctggac gggaaagagg gaacaggcta ggatatttcc gcatcgtgca
     4441 caggccttgg ttgccggaga tgcaggcttt ttaacgcatg aaaagcttct ctcccggtct
     4501 tccttccgcc gaccccaagc tcacaggaaa tttccggaat accggcgatt ttcgtaatcc
     4561 gttggggttc tgatcttgac aactatcatc tctttttaag gcctggagtc tttgtacctc
     4621 ccatgggagg ttggcgcaaa acgcccgttg cactgttgat aatcagtgcc atagccgccc
     4681 cggagtcgca ctctcatgag tgcgtggatt gaaactggat agcacgccgg atgctgttgc
     4741 cgtcccaaag tcgcactctc atgagtgcgt ggattgaaac gcctgtaagg gaagaggtgt
     4801 gggaaacgtt ggaaggtcgc actctcatga gtgcgtggat tgaaaccgta cttattgaag
     4861 cttccccgga cggaggaaag tcgcactctc atgagtgcgt ggattgaaac cctgatttcg
     4921 taggcttcct gttcttcgcg ggcggtcgca ctctcatgag tgcgtggatt gaaacaaggc
     4981 tgtcacgaag gccggaaatt gtcacggagt cgcactctca tgagtgcgtg gattgaaacc
     5041 ggcctgtatc cctgtccgaa ctggacaagg acagtcgcac tctcatgagt gcgtggattg
     5101 aaactggctg ttcagcacgc cgcccgcgga cgatacgcgt cgcactctca tgagtgcgtg
     5161 gattgaaacc tttttccggc tcctttccgc aatgcgcatc agggtcgcac tctcatgagt
     5221 gcgtggattt agatgtcgta gattgaacgg cctaatatcc ctagaacgag aaggagcatt
     5281 tggctataac gctcattaaa aacacccttc atttttagtg aagggtgttt tgacattcgt
     5341 gaaaagaaac agctcagaag ccttcaatca tttccggggt taatcccggt agatcatatt
     5401 tctttacaat atttccatcc tcggagattt caatggagtg gtggactttc gcagaagagt
     5461 attgaccact tttacagtta tgctcccacc aaatgacttt ttgaatagac atgctgcctt
     5521 ccggtcgagc agaggaggcg tctccttcaa acatctgagg aagcacttgt ttgaaaatgg
     5581 aggcatcttc atccgagaag cctgtgagtt ctgccagctg tggattgatt gctccaaaga
     5641 agacataggt tcccttgtcc acccggtgct tcatgcccat ggtgtcggaa gattttttgc
     5701 tgccgtctcc gtcattgctg acacttttcg taatttgtgt actagtgata ttcaccgtat
     5761 ctttgctaaa ggctggttga atggtgactg ggcctctgat tcctatggag acgccggtgc
     5821 cttttgccgc atctttgtct tttaaggcaa agacctgtcc aaaagctctc acatcatacc
     5881 attcctgaca ggctttttgt gccagttcag cagacgtacc tttttgtttc aggatggaag
     5941 tagcacgttc tttcaatgtt ttatagttgt ctacacgtct gtcgtctgac tgaacaaaga
     6001 tgttttgccc catgtccatc agtttattgc ggatttttct tttaagacag acatcagaga
     6061 tttctccgag tccttccaag gtggttctgg gccggttgcc atcgagaggg tctccgttgg
     6121 gattagcgtt cgtaacggta aagatgacgg caaagtcgat tttatgatta aggctcatga
     6181 tgtggtttta tagtgttgaa ttaatgtttt attctaagat ttattttgcc agttcagcat
     6241 ttttttcttc tttagttttg taaataaagg ctctctggca gtggtaaccg aggaggtagg
     6301 ctccggttaa tggcccgtta tccatgaatt gtcccatgag gaatgtttcc atgatattgt
     6361 ctatgtaagc atcatagctt ctcaagaaag aaacggtgtt ttcatttttt ttcaaaatgt
     6421 tgcgataggg ctgaatgctt ttttccagca tggaccatgt ttctgacggt ctttgctgga
     6481 accgctgcat aagtttttcc gcattggtgg ggcgggaggt tttcgtgata ttcaacgctg
     6541 atgtttcgat ggcttctgca acggctaaaa gacggccgaa gaggtaactg cgatctgtga
     6601 tttttggatc aaggctcatg gtatctgagg acgaagtatt tgtttgtgga tggttataaa
     6661 gataaacgga acaggatact cccaaggtca tttcccattc ccaccgttcc atggcatgcc
     6721 gtttggatgc ctggatgacg cattgctttt ccaggtccgg agggatgggt agattgtaaa
     6781 gaatacaggg tagtagtctg gagagggcgt gtttgcgtag tttatcgtct acctttattc
     6841 catacgctgc tttgacaatc atggagatag acggcgcgcc aatataagat cggtcgtttc
     6901 cttccttgtc tttaggaagg tgctgtttcc aactggcttt ggaatgccag gaaagcaggt
     6961 tgttaaggta ttcggagacg gtaaattcct gaaagctgga gagggacatg cgccccggcg
     7021 acgcagaatt caagctgaga acaagaacac ggtttttaat atcgttttca ttcaatgatg
     7081 tccgcagcag ctcttttcct cctccggaga gttctttttt aaattgctta accaattggg
     7141 tgacctggtc attcggtgtt ggctggtcgg gcgtgtagtg ttcccattcc tgcacattgc
     7201 cgcagggaga cggaactttc aacagaccgg gagaccaggc gagaacgaca agatctccat
     7261 cataccagcc ttggcgggag ataagccagc gaagcgcgct gtgggctttt tgggtgactt
     7321 ccgtacttac tccgcacgct tcatccgctt caatgaatct gccgcggtag gtatagccgg
     7381 aactgtcatt ggaagaaatg agtttagcct tgtctccggc gtttctgatg cgtgccgggt
     7441 gagtggttgc cagaggtgct gtttttccca ggacatagca catcccttct ttgttagcct
     7501 tggattccgt ataaattcgc caggattcgt acatggcggg gtcttcatac acttccggag
     7561 ggtttccatc cttgttgttg atcttccatc ttatgaacgc attttcttgg gtggacgctc
     7621 ccagaatttc aaagatagcg ggcatttcct gtttatcccc tttccataca ggaagcagag
     7681 tgttggtgga atcttccaca tgtaggattt cctctttgat caagtccctg atcagggatt
     7741 tcttacgaat ataggccaga acggcgttca gtttgacatt cccggggtca aacgccgccc
     7801 attccgataa ttgctgttca tattgttcgt aggatgcctg cagggttttg agttgtttgg
     7861 tccgtttggc cggggattct tccggaggga aatagtcagc gttatcacct gccacgtatt
     7921 ggagcttgtc acacagaggg tgggcacagg ctccgctggt gcgtcctcct gaggcttccg
     7981 taacggggat gatggtgagt tgactgttct gattgttgag ggatgcatca attagctctc
     8041 cctgtctgtt caatgtgatg gtgatgtgag cattctgcat gctgtggtac agcggcagaa
     8101 gaggagtaat gaggcgttct ttcctgttgc cgtgggccgg ttctttgtca atacgaccca
     8161 cataatcacg gttttgttca taggtggcgt ataaggcgta catccagttc atcgttttct
     8221 ttttcctttc attctcccag gctcagatca ttcagtccgc tggatcttgg cgggcatgcc
     8281 gccatggggc gtacatgctt gacaatttcg catgcttcag ggcgcacgaa gcggatcgtt
     8341 ccattttcca tgaccggacg ccaaaaacgt atggaaagct cctcttttcc cgtttcatcc
     8401 ggataattga aaccatggaa catgagtccg aaggaggtgt ctccatattg atcatagaat
     8461 ccctttcctt ctccaaaccg gcatggttcc acgtatccct ggcattccct ggttcccaat
     8521 gcgatgtccc tacgtcctcc gcggtcaatc atgcgacggg caatttcata atgcttgccg
     8581 tcgatccggt ccttggacat atccggacgg tacatgttcc attcaaaatg agccttgact
     8641 tgatattcca catcttgaag gtaggtatat acggataaat ccgccccggc ttcatgatat
     8701 ttgatcagtt tcatattttt gggaaccaga cggatagggc gcattattct cacctcgtca
     8761 attaaccaga tcagggtggg tttccagtac accgccttta caattccttt cagggcttca
     8821 taagtgggga tttgataaga acatttttct ccgcctatcc gggtgagagg gtcggagaaa
     8881 agcgcatatt tcccgtagac cttaaattcg atttgattaa gcatgaatat aaagctgttg
     8941 aaatttttaa atctgggaaa cggtcagcgc cccggaagtt tctccgggat ctgtgagaaa
     9001 gcccagaaat ggatcatatt tattaggtat tgttgtataa atatccactc cttcctgtat
     9061 acgcataatg tctcccgaat tcaagtattt ttcaagaagt tggctggaaa tcgcgacgga
     9121 ataggctctg gctttttgga gccattcatt ccattgttcc cattcttcat ccgtatgagg
     9181 attcatattc caaagctttt ccctgcattc ttcccctctt ccataagggg tgataacagc
     9241 tattgtatca tccttgatcg gatgatattg gcgtgctgcc gaatcaaagg cctggaaaag
     9301 acggagggga gcatatttgg actgctgacg gggatgtgtc atcagtttga aattttgaga
     9361 tagcgcatcc tccatgttag tggcgacgtt ggagcatata aacgggtagg atagatgatc
     9421 atgattatga gagcgggtga gcttgccgta gaattcctta aaatagagcg aaacggattt
     9481 atttcccaga attcctcctt cttcaggatc acaaatcatt cccatagcaa tttcccttcc
     9541 gatttcaagt tccggtagaa aatccagtct ttccagattg cctgcgggat taatcaggat
     9601 aactcttccc ccggaacggt tggcgtccct gttgcatcgg cctgccgcct gagtgcagga
     9661 atccaggccg gccacgctcc gtatgattac gtcaaagtct acgtccactc cggcctcaat
     9721 gaggccggta ctgatgcaca caacggcttt tcccttcctt ctggcctttt ccagagattt
     9781 tttgtcaata cggttcgtga tgatttcttt cctgtgggca gggcacatat tggtgctgag
     9841 gtggaaaacg tccgcctctg tattcaggtc ccggcaggcg caggccagtt tggaggccag
     9901 ttttttggtg tttacgatga cgagggtggg gcctttggaa gaagcttgct ccatgatgaa
     9961 gtccctcatt ttttctacag tccagtaatt ggagggtttc cgttcacaga caacctcgca
    10021 ttgacggaat tggctaaatt gatcgaagta agcttggctg tccggaataa taggtgtttc
    10081 gatgagatga gcatgcgttc cgtcttccgt ggcagtttcc ggaggagtag ccgtacagcg
    10141 gatggtgctg ctgtggcaga tgccggcgag aaaatccagc gaatgttcaa aaagccgtag
    10201 tgaggttggg ggaatagctt gcacttcgtc aaaaataaca acggcatttg ccagggagta
    10261 catgcgccgc acatgctgtt tgccggatgc atataaggca ttcaagtatt ggacggatgt
    10321 agtgagtaca atgggaacgt tccatgtttg agtggcttcc atgtaccagg tttttctttc
    10381 cggtgaccat ggttccagtt ctccctcctt gttaagggtt tccttcacca gattggaatg
    10441 atgctccagg atgcggtctt tatattcatt tccaagtact ttccggatgg cattggccgt
    10501 ttggtcaaga atggaggtat agggaatgat gtaaatgatg cgtgacaaat ggtgccgccg
    10561 tgcatgttcc agggcaaatc taaggcttga gagggttttg cctgcaccgg tgggaagtgt
    10621 aaggttccac ttgccaactc gtcctggagc agcttgaagg cactcctgcg acatggctgc
    10681 ccggctttga ttgatgggag tatcagcctt gaattgttcg agatgtttat tcaagttttt
    10741 taacattcct tcccaatccg gttccggaat gctttcgtag cttccttttc cggaagcact
    10801 agtgtaatcc gcttcgataa ggctgctcaa gcagaaccgt gcgattagtc cggcatggaa
    10861 tgcttgtttt tttggacagt ggccaaatag ggtggtaagg agggtgtaaa cattcttgaa
    10921 ttccgtggcc agttccgttt ttttcaggag agagtagtaa tgctctttct cctgaaacgt
    10981 caatttggac aggatttcat caagatgggt tttctcgccg gattttttca tgcgcttgga
    11041 gaatacgtca tctccgggca tcgtgccacg gaagcagtca atgagagcag ggttgtgatg
    11101 ggaggcaata cataaagcaa tgatttgccg gaagataaaa aattcttcag agttctccgg
    11161 gatgggaaaa taattccaga caaactgggc tccagcagtg gaatggtcta tgccgccttt
    11221 tggaattgga ggactgatat gttgagagag agcatccatg tattgctgaa agtctgcact
    11281 cattttgccg aagtcgtgca tgccgccgat caaggctcca aattgcgata gccctaaggg
    11341 agcaagcagg gtggacatcg tacgggcaca ttcttgttcg tgagtggaaa gagactgatg
    11401 ggggcgagcc tgatacatgt gaaactatca taggttccat atgatcattt gcaatatatg
    11461 aattgattaa tttagtattt tatttagtgt ttcaccctga acagaatttc caagatgtct
    11521 tcttcctttt tttcagtgcc cttggcggca ttacagcatt acttgtattg tcccaggcaa
    11581 tgcgcactga ttcatctgga atgcgcgtgg gctgataatc aatttacggc ggaggggaat
    11641 gtatttcatg aacgggcgga ttccggcatg acggaaaacc gcgggcgaaa gaggatcctg
    11701 cgttctcttc atatttcttc cctccaatgg ggaattcacg gcattgcgga tgtcgtggag
    11761 gttgtttatg ctgagaaagg cggcattccc gtatccatga cgcccgtgga gtacaaggtt
    11821 ggcaagccca aaccgcaccg tgcggatgaa gtgcaggtgt gcgcccaggc cttgtgcctg
    11881 gaagaaatgt ttgggttgga gattccctcc ggttttctgt tttatggaaa gacccagaaa
    11941 aggatggagg tggtatttga tgaagagctg cgtattctga cagccgaggc tatcaggaat
    12001 gccagggagg ttttaaccgg ggaagtgacg ccgccgccca tgtattccaa agcctgcaaa
    12061 tcctgttccc tggtcaatga ctgtcttcct aacgtttgcc gggagcacag gcagaccatg
    12121 aaagcccggg tggatagtgt gtttgaagac agcttggaaa caagtgaagc cgatgattta
    12181 tgaagaagca tttaaataca ttgtttgtga cgctggaagg atgctggctg tccaaggatg
    12241 gcgatactgt aaaagtcaat gcagaaggaa cgtgtctgct gagggttccc ctccataact
    12301 tggagggtat tgtgacgctg ggatgggaca taggagtttc cccttatttg atgggagcct
    12361 gtgcggaacg cggcatcacg ctttccttct gcaatcctta cggtaaattt ctggctggcg
    12421 tacagggctt catgaaggga aatatcctgt tgcgccggga gcaataccgc tgggctgatg
    12481 atgaggcaag atgggttggc gttgcccggg aaatgatagg agccaaaatt gcgaattgcc
    12541 gcagtctgct tttaagggcg caaagaacat acgggaaaga tttgtcggag gcgatcgatc
    12601 gtctggcggc tttttgccgc atggcccgtg gatgcaattc cggagacatg ttgcggggca
    12661 ttgaaggttc cgcagcggaa acctattttt cctgcttgtc ccattgccag aggacggaag
    12721 atccggagct ggtgtttgaa agccgcaata gacgtcctcc caaagattgt tttaatgctc
    12781 ttttatcttt tctatacagt ttgttgtgcc atgacgccag gtccgccctt gaaagttccg
    12841 gcattgatgc cgccgcaggc tttctgcacc gtgaccgccc ggggcgtccc ggaatggctt
    12901 tggacctgat ggaggaattc cgggctcctc ttgccgatcg cctggccctt accctgatta
    12961 accgcaagca gatatcctca gatgattttg acaaggaaga atccggagcc gtttttttga
    13021 aggaagattc ccgtaaaaaa gtattaaatg cctggcagga acggaaaaag accgttctgg
    13081 tgcatccttt tcttcaggaa aaaaccaccc tgggattgct gattcatatt caggcccttt
    13141 tgttggcacg ccatttgcgt ggggatctgg atttctaccc tccgttcatc ggcaagtaaa
    13201 acgagctgtt gtcatgtata ttctcattac gtatgatgta gctacggatg acaaggccgg
    13261 gcagcggcgg ttgcggcaag ttgcccgagc ctgtgaaaat gtcggacaga gagtgcagaa
    13321 ttccgtattt gaatgtgaat tgcctcctgc tcaattggtt gacattagga acaagc
//
LOCUS       DAWEMX010000066        13314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1956_length_13314_cov_12.993212, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000066 DAWEMX010000000
VERSION     DAWEMX010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_13314_cov_12.993212"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2732 a         3399 c         4119 g         3064 t
ORIGIN      
        1 tgacgcctgt caggaactgg taggcaatga ccagcagaat gcccgcgaaa tagagcctgg
       61 ttccgctggc gagcacccgg ctgatcagga agaccatgga ggctgtccgc cgcgtcagga
      121 gcccgaatct tttttccagg tattcataga tggaaacaac cttgtagtca taatagggct
      181 tgagaaagag cctgccgacg atgatgcggc ccaggatggt gccgatgcag agctgggcgt
      241 atgtgaagtt gcgggtatgg tacccttctc cgggagcccc caggaaggtg gccgcgctga
      301 tttccgccgc caggatagag gccagaatag cccaccaggg cagggagcgg ttgcccaggg
      361 catagtctgt cagggagttt tgctttcgtc ccgcataaat gccaatgcag aagatagcaa
      421 gaaagtaaat gacgatgacg aaaaggtccg tgagcatgcc tagaaagaga aggccttttc
      481 cggaatggaa attccggaaa aggccccggg cttaatgaaa gggtcaggag tggctcttgt
      541 gttccagcga ggctgaaacc aggccggaaa agagcgggtg ggcccggttg ggagcggact
      601 ggaattccgg atggtactgg caggcgatga agaagggatg atcgggaagt tccacgactt
      661 ccaccagtcc gtgttcgtca ttgacggcgc tgatgaccag ccccgcgttt tccagttcgt
      721 cgcggtaggc cgggttgaat tcgtaccggt ggcggtggcg ttccgtaacg ctgtccttgc
      781 cgtaaagctt gtgcgccagc gttccgggcc ggatcagggc cttgtaggcg cccaggcgca
      841 tggtggctcc catggccttg atgccttttt gctcttcctg gaggctgatg acggggtggg
      901 tggttttctg gtcgaattcc gtggagttgg cgtcatccat gccgcagatg tggcgtgcgt
      961 actcaatgac ggccacctgc atgcccaggc aaatgcccag gaacgggatt cctttggtgc
     1021 gcgcgtattc caccgtctgg attttccctt caatgccgcg gtctccaaag ccgcccggca
     1081 ccagaatgcc gtccacggag ccaatcatgg cctccgcgcc cttttcctca atttcttccg
     1141 cgtctatccg gagaatttcc acgcgggcgt cattttccgc accggagtga gtgaaggatt
     1201 catagatgga tttgtaggcg tcctggagag cgatgtattt acctactacg gcaatcctga
     1261 tggagtggct ggggctgatg accctgccga cgtaacgctg ccagtccgcc agattgggcg
     1321 ccggaacgtc cagtcccagc cgcctggtga caatgcggtc tattttatcc tggctgaggt
     1381 ccagagggca ttcgtagatg gtgtttttga cgtcgcggaa ggcaatgacg tttttggctt
     1441 ccacgttgca gaacatggcg attttgcggc gttcgtcttc actcatgtcg tattccgtcc
     1501 ggcagatgat cacgtccggc tggatgccga tttcccggag cttggcgacg gattgctggg
     1561 tgggcttcgt tttcatttcc ccggcggcct tgatataggg gagcagggtg acgtgaatga
     1621 agcagacgtt gtcacggccc acttccaggg cgaattggcg cagggcttcc aggaaaatgt
     1681 ggccttccat gtccccgacc gtgccgccga tttccgtgat gatgatgtcc acgtctgatt
     1741 tttccgtgac ttcatagagg cggtttttga tttcatccgt gacatgcgga atgtattgaa
     1801 ccgtttttcc cagatagtct ccccggcgtt ccttccgcag cacgctttca aagacctgtc
     1861 cggaagtgag gttgttgagc cgggagaggc tgcaatggac gaagcgttcg taatggccca
     1921 ggtccaggtc cgtttccgct ccgtcgttca agacgtacac ttccccatgc tggaacgggc
     1981 tcatcgttcc ggggtccaca ttcaggtagg ggtcgaattt ttgaagggtt accttgagtc
     2041 cgcagcgttc cagcagcgtg ccgatggatg ccgccgccag tcctttgccg agagaggaga
     2101 caacaccgcc tgtgacgaag atatatttca tgatttgctt cctagtgtat gtttaataag
     2161 atttgttcca cctgttccgc ctgttccggc gtatccacgc ccacactgac gtggtccgtc
     2221 aggatgaccc ggattctggc cccgttttcc agtgcgcgca gttgttccag ggattccgtc
     2281 tgttccaggg gggtcggggt ccagcgcaca tagttttcaa ggaagtcgcg gcggtaggcg
     2341 taaataccca gatggcgcaa aggcggcgct acgcgtggat ggcgggcgta gggaatgacg
     2401 gaacgggaga agtacagggc gtctccgtta cgggcaaggg ccactttgac aatgttcggg
     2461 ttgtccagat cctcccgggc gctgatgggg caggcgacgg tggccatgga gagggcttcg
     2521 tccgagacca gggcttccgc cagcctgtca atcagggccg ggtcaatgag aggttcgtcc
     2581 ccctgaatat tgacgacatg ggtggcggca gggaaggaac gtaccgcttc cgccaggcgg
     2641 tcactgccgc tgggatggtc cggagaggtc atgatggccc tggctccaaa ggatacggcg
     2701 gcgtcaaaaa tgcgctggtc gtccgtggcg atggcgatgg cgtccaggcg ggaacagcgg
     2761 cagacgcgtt cccatacgtg ctggacaaga ggttttcctg caatgggatg aaggggcttg
     2821 cccggaaatc tggaggaacc ccagcgtgac ggaatgaggc cgatgatgtg atgggaagta
     2881 tctgctgcca tgggaaaagg aaccgccccg aaaggtagca actccctttg aattagtcca
     2941 gcctcaagca tagggaacgc ctgtttttcc cgaggaagga acagtcagcc ggaaatggat
     3001 gccgttgcgg cggcttgccg tttttcctgc gttttccgga tcatgagcgg catgtattcc
     3061 ttgcggacat ggagggatac atagaggccc tccctcttgg cgcaggagta gagtactttg
     3121 atgatggcca gggatatgat gtccatcgca atggtgatgc tttccccaaa gatcaggaaa
     3181 aagaagattg gtttggtgaa atagacggcg gaaacataca ggatgccgag cttgtattga
     3241 tggttgcggt aaattctact gacgatccat gcgtaggtca tgccaacgac ggacaggata
     3301 aggtacaggg aaatccctat gtagccgagc gtttgaattg tggaaatggg gccgctgtgc
     3361 cattggccgt tccacgcaaa ggcgctttgg tctttgctca ggttgtaggc ttcttcatac
     3421 acattggctt tcacgatgct gatatcccgt gaaaagccgt ctccgaatat tttgtcctga
     3481 ataaagtgtt cacggtcatc cagagcccag ctccacattt caaagcgcca gttgatggag
     3541 tcttcggcgt ttgccttggc ttgcgcgctg acgtccagga aaggaacggc tgagagggtg
     3601 cgctggatgc cgaaaggcag agaatggagc atgcccgcag aggagagcag caagagcaat
     3661 agcactccga ggacgggagc cagaaggcag aagaaccatc ttctgtaaat tagggatatg
     3721 gtgaagaaca ggaacagaac ctgggccatg gcggagcgaa atccgcttaa caggatgcca
     3781 acggttccaa gaagaagcaa cgccccccac cagggacgtt tgatcatctg ccacacggag
     3841 tagttgatga tgagcagctg catcaccaag atagaaattc tgaggaagga agagttccgg
     3901 gagttttcgg agagtgtgcc tgcggagccc gcgctcgcgg caagatccgc cccggcgcta
     3961 tccggagaaa ggaggctccg ggcggttagg ataagggtaa aaacgatggt caggaaaaca
     4021 gcccattgaa gcactttgcc cagttcatgg ctggtcgttt ttaacgagga caggcagaga
     4081 taggccagga gggcctggag gaaaaggatg tatcccttac ctccgtagta gtcttccagc
     4141 acatccagcc ccagagcgaa agggtggctc aggaagatgt agcccacatg catgaacagc
     4201 acgaggacga cagcgtccag aaggggggcg gaattccacg tcaagcttga acgcctgagc
     4261 atggcgttcc acaagtaaca aggaagggtt attccgtaag caaggagggg agcgtaatcc
     4321 cttaaggagg ggatgggcag gaaaagaatg ggtatcagta tccacagata ccacactttt
     4381 cccttcatca ggaataaggc ggccaggata aagggggaaa cagccagaac tatagaaagg
     4441 ggatcaccgt ccgccgacat gatgccgcct atgatggcta aaagaatggc aacgactgaa
     4501 accagtacaa ttttaaataa gttaccattc atagggctac ccgttattcc cgaataaaaa
     4561 acagtagcgg gaaagagaga tggatggtgg agtatagggg attcgaaccc ctgaccttct
     4621 cattgcgaac gagacgctct accaactgag ctaatacccc gtgggaaata ccggaaagaa
     4681 tttagttcat cccgcgtttg aaagcaagcg gaattcgtca ggaaaggcaa ataagaagga
     4741 agatgccgcc tttccttatc tcgtgcgggt gtttcatgac tgccggaggc cgtccgcagg
     4801 gaagatggct atttccggga acgcccggct tttacgggaa aggaggaacg ccccggcctg
     4861 gaagccggag ggcctgcggg aatgttcagg tgtaaaggca ggcggacctg atgtttccga
     4921 tttttgcgaa ggcccgtttg ccggaattgg agcgtcagcg gccctgcttg tttttaatgc
     4981 ggtttctgag atgggcttct tcaaaaaggc cgtccatgta taatcccacg gcttgagctt
     5041 ttctgagact ccgggctgca tccctcaggg ccggaagcgc ttcttcatag gatggcattt
     5101 ttcctttttt aacaggggat gcttccacaa ggtgccaccc ccaccggctt ttgagaagta
     5161 ccggcctgtt gtccggaacg tctgccaacg ccagacccag ggtttccttg gcgcgggcgg
     5221 ggcttatcca gccgagttcc ccgccggcgg gagcggaacg ttcgtcttca ctgaattccc
     5281 tggcgagccg ggagaaggat tcccctgcct tcagccgctc cagcagcgtt tccgcggttt
     5341 gccggacctg ggcttcttcc ctgttcaggg tagccaggaa gatgtgcctg gttttccgca
     5401 gatcaggagg ggtgagctgg tcccggatca ggttgtagta ggttttcagg tcttcgtcgg
     5461 acgcttccgc cgcctgggcg gttgcccggt agagctgttc cgtctgtttg agcctggccg
     5521 ctattttttt ttgaaattgt tcctgcgtca atttctggcc atgcagggtt ttcaggtatt
     5581 gttccgtgtc tccgtcaaaa cgggaccgga tttgttccac ggcgcgtgcc gcttccgcgt
     5641 tgcggttggg gagctggagg tcattgacgc tggttttcag gcgcagaagg gaggagccaa
     5701 tcaggtcata cctggcccgc ggagccaggt cgccggggat gtccggttca tgagtaagtt
     5761 tgatcccgcc ttccatatcc agcacgggag gctggcgcag cattttcagt tccgtaattc
     5821 ttcgggaaag ctggttggcg gtgacgggtt ccccgtacac ttccgctatc cgggcggagc
     5881 tgtctcctgg cggccctggc aacggtttca ggtacgcgtc catcctgccg gccagaaagc
     5941 cgtgccagac aaacaagtct cccaccagat agagcaggaa ggctccataa atcacaagcc
     6001 tgagcgtgaa ttttccagtg ggagacatgg caggagaaga agggcaaaga gcttagtttc
     6061 cgccgccgga gtctgccagg cgggcggatt ccggaggaag gaagttgcgg aatctcttct
     6121 tttcaatgca cttcatgtag gcttcttccg gggagacgat gccctgctgg agtttttgcc
     6181 agatggcgtc atccatgaat tgcatgccca tggccttgcc gccgacgatg acgtcaggga
     6241 ttttctgcgt ggcgccttca cggatgatgg ccgccacggc cggcgtggca aagagaattt
     6301 cattgacggc tacgcgtccc ggtttgtcgc tgcgcttcat caggagctgc gccacgacgc
     6361 cgcggaggga acccgcaagc atggtgcgca cctgggattg ctggtcggac gggaatacgt
     6421 caatgatacg gtcaatggtt ttgctggcgt tgttcgtgtg cagggtgccg aataccagaa
     6481 ggccggtttc tgccgccgtg agcgccaggg agatggtttc caggtcgcgc atttcaccca
     6541 ccagcacgat gtcggagtct tcacgaaggg aagcccggag gccgtctgcg aaggaaggcg
     6601 tctggagagg cacttccctc tgggtgacga tggatttttt attgggatgg acgaattcga
     6661 taggttcctc cacggtgatg atgtgccgcg cctggttgct gttgatgtag tcgatgatag
     6721 cggccagcgt ggtggattta ccggagccgg tgggccccgt gaccagcaca aggccggatc
     6781 tcatatgggc gaattccttg accaccgtag gcacgcccag ttcttccagc gtggctattt
     6841 tggtgggaat gaggcggaaa acagccccca ggccgtgctg ctgcttgaag tagttgcagc
     6901 ggaaacggga tgtttcatcc atttcatagg caaagtccag gtctccttct tccagatatt
     6961 tctggaaggc ctggggatcg catatttccg cgagaaggtt tttgaacgcc tggccttcca
     7021 ggttttcgtc ggagatggcg gagatggtcc cgttaacgcg gactttagga ggctggcctt
     7081 cgctgaggtg aaggtcggaa ccgcccgctt ctacgaggta tttgaagtat tggtcgatga
     7141 cagccataac aggagtgttt caaaattaaa ggttacttga ttcccaggaa ggatttcatg
     7201 atggttttgt cttccgcgcg gaaaatgcag tcttccgtgg tgatgaggcc cgcctggtgc
     7261 agggcccgga gcgagtcgtc catcaggatc atgccctggg ctttccctgt ctggatgcag
     7321 ccgggaatca tgaatgtctt gccttcgcgg atgcagttgg cgatagccgg cgtattaacc
     7381 agcatttcaa tggccagagc gcggccgttg ccgtctgctc tgggaaccag ctgctgggag
     7441 atgatgccgc gcagggattc gctgaccatg atgcggatct ggtcgcgttg ttccaccggg
     7501 aagacgtcca gcacgcggtc caccgtacgg gctgcggaac ccgtatgcag ggtgccgagc
     7561 accaggtggc cggtttctgc cgccgtgagc gccagggaga tggtttccag gtcgcgcatt
     7621 tcgcccacca tgatgacgtc agggtcttca cggagggctc cgcggagggc ccgcgcgaag
     7681 gattccgtat gtttatgcac ttcacgctgg ttgacgtggc agtttttgga gggaatcagg
     7741 tattcgatgg ggtcttcaag cgtgatgatg tggtcgtggc ggtcctggtt gatgaaatcc
     7801 acgatggagg ccagcgtggt ggatttaccg gaacctacgg agcccgtgac taaaatgaga
     7861 ccgttggtgt agcgggtgag gggcaccacg tattccgtcg gcaggcctat ttcctccatg
     7921 gtgcgcaccc gcgtgttgat gatgcggaag cagatgtcat aacccaggcg ctgtttaacc
     7981 acggatgcgc ggtagcggcc aaagtcattg gcgtaggcga agtccacgtc cccccgttgc
     8041 tgaaggcggt tccattccgg gtcttccaga aagcccctcg ccagctcttc cgtgtcctgg
     8101 ggcgtgagat tgggcgcttc ctcccagatg gggagaagct ggccgaaccg gcgccaggtg
     8161 ggacggctgt tgacggcaag gtggatatcg gaacagtcat aatcctgtcc aagtttgaga
     8221 tatccgtcga cgtggggcaa aatgtattcg ctcatgttgt gaatgcagca aactgataaa
     8281 gctgcatatt aacgaaaaca gagctttttt gaccagcaaa aaatgtctgg gaaccgattt
     8341 ccgtgaaagc ggctttgccc tgttccgcga cgggtgagga atttcgagag gggaagtttt
     8401 gcggaagagc tgcggacgtt tatttccggg gaaggggagg atgatggaac ggggggctgg
     8461 cgcagcaggc cggcttttta cgccaggcat gatttattgt tccctggaag acgggaagga
     8521 tatggggagg ttctcattta acggcgcggg aacatcctca ttttttacgg cagcggagtt
     8581 cccgaacctt tttcaagaag cggcagaaaa ccgctgtatt ggtctgaaaa tcctgttatt
     8641 tgaacaggtt gaggaatttt ttgccggcca ggccgatccc catttttttc agggaaggga
     8701 aagccagggt aatcagaatt tcaacgatga aacggaagat ggaggaatta acccagcttc
     8761 cggaagtgcg cggctgcggg ggaggggccg gattgctttt tttccccgca aacatgcgga
     8821 tgagaagaac gcctcccacc gttccgacaa tagagccgat tacaggcagg gacatggcct
     8881 tttttttcaa tttacggaag tggtcggcct gctgctgcgt cttttgcacc aggctgtccg
     8941 cctcattgac gatgtccagc cgggaggaag ctacccgcat caggagtgcg agcttttctt
     9001 gagagccatt tgcagacatg ataaatccgt tttaagttct tccgccgtcg ccggagcgat
     9061 aggcgttaca tgggttcggc tgaaaaggat gaacccggct gcggcggcca ggagatgaaa
     9121 gcccgtggtt acggcaagcc cggcgatgat tccccagaaa tacgccatga ccacagtcag
     9181 aaggcaccac agaaatagat agccgaagaa ggccatgaag gcgcccatca ggagaaaggc
     9241 cgtttttttg cccagcctgt tggacgcttc cttcagttcc agtttgagca gtgcggccag
     9301 ggcttccgca tgggccaggg ctgccgtccc ggtttcccgg agggaccgga ccatggccat
     9361 cccttcttcc ttttcctcca caaggggggt gacgaaggtt cgcttgagtt tgtcgatcag
     9421 tcccatggtt cggtttgaaa ggggagggag gacgccatgc gcatgacacg catggcgcgg
     9481 aatgaaggga gagggttaac gacggcggat aagcagaccg atgagaaggc ccacgcccat
     9541 ggcaatcagc acggactggg tgggcttttc cttcacgtat tcttcgcctg cttcgtggaa
     9601 cttcttggcc ttgtcacgga tgacttctcc cttttcctgg gcgtagtcgc agatggtgct
     9661 ggcctgttcc gtagccgcct tgcggatttg gtttacttta gctgcggcga aggccctggc
     9721 ctgttgaaat ctggtgacgg caagctcgcg ggcggaaggg caggagcagg aagtgtcgtt
     9781 tgctgctgcg gtcgtttcaa gttcggtttg gttgtcttgc atggttgatt agagtattaa
     9841 gggttggttc ctgttgaaaa acatgtcgac ttacatgttt tcgcctctca cattggaaat
     9901 tatttagcac gcttcccact attggcaagt gtgtaaagcg gcttcacccg gaattgtgcg
     9961 gtctctcagc ggcggcttcc tgaaaaaccg ccgcgggggt ccggtttgac ttggggttcg
    10021 ttaggattcc ggtacctcag aatgccgtaa tccctcagat tgcggatgaa ttcctgagcg
    10081 ttggaccgga agcgagcatc gtacataagc agcccaagaa agccctggtt tttacgtgca
    10141 tcagccgtga aggaggcgat ttgttcagat gctttcctca gagcggccag cgtagggggg
    10201 attgccttga cggcggggtc cagagtgttg agcttttctt cagctccggc aatgagtttg
    10261 cgggcatccc ggacggtgtc tttcatggcc gcgaccgagg agcgcgtttc ttccatgagg
    10321 ccggggagct gttctcccgc ccggtttatt tgagacagta cgctgccgag gttctggagg
    10381 ttctcccggt tcaggatgcc ctcgttgatc agcctggtcg tctgggccag acctttggcc
    10441 gcctctccga tattgaggat ggcgtcgtca atgttgtccg aatttttctc gatttgcttg
    10501 aggattttca ggatttcctc tgtggcgccc acggcattgt ttttgatttt gctgaaatcg
    10561 ctttccggct gaccgatgat ggtggcatgg ggaaggatgt attcatgcgt gggctgggcc
    10621 ggagggacga tatcgatgta tttgtcgccc aggatgttct gcatgtccac ccggaagacg
    10681 gacccttgct ggattttcac gtcgctcagg atgttggctt ccagcatgac ttcattgccg
    10741 gaaggcagga gttcaggggc tttggtgact ttccccaccg tgacgccgcc caggcggatc
    10801 tgggaatcct tgatgagccc cgccgcatct ttgaaaatga tgttgatggg gtaggattgc
    10861 tccttggatg ttttgatgtt gccgaatccc aggatgactc cgataatcat caggatcccg
    10921 gcgatcagaa agatgcctac ccaggtttcc ggtttaagtt tctgtttcat ggctctgtgg
    10981 caagatggtt attgtttgcg ttcccgttcc gcccgttcca gcagaacccg ttggaagttt
    11041 tcatttttgc cggacagggc tgcccagttt tccccggagt ccccgtacag gaatttttgc
    11101 aggatttcgt ctttggagtg caacagttct tccggagttc ccgtccagta aacctcaccg
    11161 tttctgagat agacgatttt gtccgcgatg cggatcacgc tgggcatgtc atgggagaca
    11221 atgacggtgg tgatgccttt gtccttgcag atttgccgga tgaggaagct gatgctgtcc
    11281 gtaacgatgg ggtccaggcc cgcggtgggt tcgtcataga gcaggcattc cggagtggta
    11341 atgattgccc tggctacggc gacgcgcttg atcatgccgc cggagaggct ggccggcatt
    11401 ttttcctgct gtcccagcag tcctacggaa tccaaagcgg ctgagatacg ggtgtccagt
    11461 tcgttttcat ccctgatccc cgcctcccgc agggggaagg ctacgttttc tcccacgctc
    11521 agggaatcga ataaggctcc ctgctggaac atgaatccga tgttgctgcg gatttgcttt
    11581 ttgatttttt ccggcgcgcc gctgatgcgc ctgccgtcta tttccacata gccgtccaag
    11641 gggtccatca ggccgtccag atgtttcaga atgacgcttt tcccggctcc ggaacctcct
    11701 attaaagcca tcagttctcc cttgcccacg tcaaagctta cgcctttcag cacttcctgg
    11761 gtgccgaagc tttgtctgag ctgatggacg cggatgaatg gctgcatggc ggcggggaaa
    11821 ggctatctca tgaatcccat ggggaagaaa gagttgagga tcatggtcag gataaagttg
    11881 gcgatgagca gggccacgga agcgtacacc atggcctgca tggtggattt gcctacgccc
    11941 acggcgccgt tcgtggtgtt cagcccctcc cggcaggaga tggtggaaat gatgaggccg
    12001 aaagcaagag ccttgaccag ggctacgatg atgtccccca tggtgacgaa tttgctcatg
    12061 aagtgcatcc agtaggcctg gtctacgtta aaggcggtga cgctgacgat gtacgcggag
    12121 gcgatgccca ccagaacggc ttccatcatc aggatgggca tggaaataag catggcctgt
    12181 atccgcggct tcaccaggta gtctaccgga tccacattca tggatttaag ggcatccacc
    12241 tgttccgtga ctttcatggt gccgatttcc gcggccatgg cggatccgac gcgccccgca
    12301 agcatcagcc cggtgatgac ggggcccagc tcccggaaca tgcccacggc gacgacggcc
    12361 ccgcccatgg tttccattct caccgtttga agctggaata gtgtctgggc ttccagcacc
    12421 gcgccggtga acgcgccggt gatgataacc accggctggg attgcacccc gatttttgca
    12481 atttgctcca caagcacgcg catccggaat tggccggacc acatgcatga agccacttga
    12541 taaagcagca atccgattga ccccaaccgg tcaaccagcc ttaaagcggc gcggccgatc
    12601 atggttgcta catggaattg catggcttct actaatcact tcttcctctt caagagcaag
    12661 cgcaatgtat gccacgggag gaaaagggag gttccgcgtc agtctgccag ccgatggata
    12721 taaggcgttt cctgcgttcc ggggatggag cgcagcatga ggcgcggcgg cagtttcagg
    12781 cgtgtccatt cggatgcgcg ggcctgccgc tgcagacggc ggatttccct ttccgattcc
    12841 gggaaccgat tcgccagctc cgtggcggat tcatgttccc ggtgcaggcg gatgagaaac
    12901 ccgtcgcgcc tggcggcctc cggagcggga gaaataaaac cggggaacag gtttgccagt
    12961 tccgtttcgt ataaatcccc cagcggcatg agatccgcgg ctaaagcggc tggaaggaag
    13021 cggggcgccg tcatgatatc cgtccccgtc agagaagaga gcagcagggc gccttcttct
    13081 tccgcccatt ggcgcatgag ccacgcgatt gcagcttttt cgtccagccc tccggctttg
    13141 gcggggaaag aaaccgtccg tacgccaatg ccagccgtgc cggcgaaggc ttggactctt
    13201 tcccgtgtct cttccggaat gtcgggcagg gaggggagga accccgtcac ttccagagag
    13261 gggcacgttt ccttcaatag ttccgccaac aggagggatg cggaattttc cagc
//
LOCUS       DAWEMX010000067        13234 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_1966_length_13234_cov_13.868655, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000067 DAWEMX010000000
VERSION     DAWEMX010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_13234_cov_13.868655"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2782 a         3460 c         3974 g         3018 t
ORIGIN      
        1 ggaaccaggg cgcggttgag ctgttcaata ttggcggtgc gggtatcctg ctggatgggg
       61 agttcctgcc cggctacatc ctccagatac cctgcgggga cggaagattg ggattcctga
      121 atagccgttt tcaggctgga aaggtctacc ccggccagtt tgagcttgtt gggatcataa
      181 atcacctggt attccggcag acgcccgccc agcaccgtga cctgccccac cccgggaatg
      241 gccagcaggc gcgtgcgctg ggaggtatcc ttgtctccag tcagggcgat gagcatgatt
      301 tcccccgtta tggagacgat gggggccagc tccggggatg tcccttccgg cagggattcc
      361 cggactgctc caagacgttc cgttacaatc tgccgggcct ggtagatatc tttgttccag
      421 tcgaaatcca cccatacgaa ggaaagcccg ctgccggagg aggagcggac gcctttgact
      481 ccggcggttc cgttcatggc ggactcaatg ggaatggtga tgtattgttc cacttcttcc
      541 gcagtaaggc cgggggcttc cgtctggatg gtgacacgcg ggaccttcag ttccgggaat
      601 acgtccacgg gaatgttctt caccacgaaa atgctgatga ttgccagcgc aatcgtcagc
      661 aggatcacag taatcctgtt gttcaggcag aatgaaatga gaaagttcat ggattctgat
      721 tgctgaatac ctaagattca agggtttatt tcaaaggaat tcggggtatt tgttccttaa
      781 tcttttattt tgtacatcgt ttagtgttct ccttcgtgga actggccgtc cgcgtggaag
      841 tgcccggctg cttttttgct tccggcctct ccggtgggca ggatgtattt caattcatac
      901 ccacccttgg tgacgatagt ctgtcccggc atcagcccct tgacgggcgt ttttccctga
      961 cgggaaggca gtgtttccac ttttttcatg acaaatgtgt cgtttcccgc cttgatgaaa
     1021 accacgtcgt tcactcctac tttgacgacg gcgctgttgg ggacgggaat gaagccctcc
     1081 gtggcggcgt catgggagta cagatccaac cgggccagct ggccggcatg tgtcccttcc
     1141 ggtatgcggt ccggggtaaa atacagggcc cgggactggg tggcgggatc tacctgttcg
     1201 gccaccctca gggagccgtt cagcagttcc gtgtttttac cgctggtcag agccagccgg
     1261 gcttttgcat aatggacggg atccgtccca taaatggtcg tggtaaattc cagatcccct
     1321 ttattggtca tgacgagggc aggtgcgccc tgttctcccc aggccccttg ggtcatgttc
     1381 acggactgaa cggaaccatc cgccgctgcg tatatgtaaa gaaggttatt tttcaatacg
     1441 ccggactcca tcaccagttt caatttattg ttgctggcgt tcagggcagc ccgaaggctg
     1501 tggatttccg cctctttgaa ttggatggaa gtattcagtt cgctgttgcg ggtgccgatt
     1561 ttttccaact gggcccggcg ttccctgagg gttccgagtt ctgctgcggc acggtccagc
     1621 gcggccctgg cctgggaggc gtttccttcc atttccacaa tatccgggga tgccagcgtg
     1681 tacagcagtt cccctttgcg cacttgttgt gcggatttga cgttgaatgt cacgcgccct
     1741 gcggcgggaa gggcgtatgt ggttaccgca tggggaggaa tgaccatctg cccgtgaagg
     1801 gttttcgccg ctccgtggga ggtttcctcc actttctcaa agcgcatgtc cagagaatgg
     1861 cgcgcttttt catccacatg cacgggaatc atgtcccccg gttggctgga atttttttcc
     1921 gcagactctt cgcgttcatg gtcgcccgtg catgcttccc cttccgcgtg ctggtgacct
     1981 tccagttcat ggtcgcctgt gcagacggct ccgtccgcat gcctgtgttc ttcgtgggcg
     2041 tggccctgag tacaggtttc ccctgccttg tgctgatggt cgtcatggcc gtgatccgcc
     2101 gtgcaggcgg aaccgtcctc gtgtttgtgt tccgcagggg caggctgttc tccgcgtgcg
     2161 gaatggagac agaaagcagg agccagtccc agcatgatgg aaaaataaag atgtttcata
     2221 ttcattaaag atggtggagg gttgatgtgg attaaaggtt ggcgtgggtg aggtagcgga
     2281 gctgggcctg aacggccaga agcttgtcca gattgtccag gaaagccagg cggctttcgt
     2341 aaagctggtg gcggttttcc gccagttcca gcagggatgc ttccccaatg ccgtgcagct
     2401 tttccatggt ccggacggag gcggcgaagg attccatgcg ctggagttcc gtgcggcagt
     2461 ggcggagcgc catctgctgg cttgcgtcaa gctggcgggc gttgaataat tccgttttcc
     2521 aaagctggat ggtttcctgt ctggacaagt cccgtgtgcc tcgggcttcc gcaatagcct
     2581 tgcggttgcg gttccaaagg ggaagattga accctatttc tccgcctact tccttttctc
     2641 cttcgtcgcg ggtgaaggag gggccgagct ccagttccgg gtactgccgg cggatttccg
     2701 ttttgaataa tgtctccgtg gtggcgtagg tcgccagttg tgccttgatt tttggagaga
     2761 cggtcaaggc tgaagggccg ggagccggta cggcggcggg aagctggaaa ctctgtttcg
     2821 tcaggaaacc gagtttccgc accgcagaag gatgcaggcc catcagcttg acgagttcca
     2881 tcttctgctc caattccgtt tccgtcacgg tttggagttc ccggatagcg tcattcatcc
     2941 gctgggaggc tacctgtcga gaggaaaaat ccacttcccc cgcgccgata agctgttcaa
     3001 tcatgccgtt ttcccgtcgg atggcatcca gccgttcccg gatgaccgtc ttgcggcgct
     3061 gggtaacggc cagtttgctc catgcctggt ccagggaact gagaaaatcc agttcagcct
     3121 ggcggagggt ccagaagtcg gcctccttgt attgttccgc cacttttttt tccagggcgg
     3181 ggaggccggt tacgggaatg gtgaagccca ggctgccgga catgttgacc gtattttctt
     3241 ggaggacttg ttcaatatcc caggaaaagg agggatcgtt ccaccagccc gattgcttgg
     3301 ctacttcatt gctggaagcc agtttcagcc gggctttgtt cagatccgga ttcatgacca
     3361 ggccgatctg gcgagcctgg ttctgagtga ttgaggtagg ggaatctacg cgggacgcct
     3421 ctttccagga ggcttcctcc gcgttcaggt cgataggcgc gctgtggtag acgctgcatg
     3481 agctgagcag ggctgccagg cctattccgg cgaagaatgg tgtggatttc atgatggaaa
     3541 agataataaa aacagttcgg tccaatcagg tttggacaat gcggcgaata attccgcgga
     3601 aacaaataaa agcagccgcc gtattaaagg cagcgtaccg caggaaacgc ccttaaggac
     3661 gtttctgaaa aagagccgtt aaatcagcag gggaagttga aagccttcat gcgtcggacc
     3721 ggtgttcctg aaggggtcag ggggaaccgg tgttgcggaa tccgatgttt ggcttgttaa
     3781 aagaagaggc cgggccagat ggtggaaatc cggcaatgtc tgccagatga cataaggctg
     3841 gggcagaggt gcatgaggaa cgggaaccgt cagatcatta atctccagct gccggtgttc
     3901 gcagaggtgc ccggccaaag ggctggaaga gccggtacgg ggcacatcgt ccccgtggca
     3961 ttcgcagggg gcggcatcct gatggcagga acaggaaaaa atgaaaattt cttcatggca
     4021 cgggcacacg gaaaccatac ccgttccgcc tacgacgacc agcagaaggg tgagcagcat
     4081 gatgccggtg cgcaatgctc taaccatggt atgccgcgaa ttctagaaag gagccgtgaa
     4141 agcgtcaaga gcaaaagtgg cggatgtctg aaccctgaag gaaaggccgt tatttttccg
     4201 ccgctttgcg gagcaggtcc acagtgtgtt caaaagctac gccgcggaca tcaatgatcc
     4261 gggagttttc cgcgattgct ccggtgattt cctcccggaa tttaccggac tgttcttccg
     4321 tgagcaggtt tttggagctc agggagcgcg tgatgagctg gaggacgtac cctgtttcca
     4381 cgaaagactg gcgtcccacg gctttcttca tggcttccat gaattccggg gcatgttcgg
     4441 gcggcaggcc ggccacggcc ttttcataag cacggatgaa ggaatccacc ggggctttgg
     4501 aaacttccgt cttggtgagg tcaaagctgg attgataagc gagagctcct tcttccgcca
     4561 gttgaagggc gtttttcccg aatttgttga cggcagtcat atccgctcct ttttccagca
     4621 gcagggccgc cacctggaaa ttaccgcgca tggctgccag catgaggggc gtaaagccgt
     4681 tgccctcccg gttgttgatg tcagcgttcc tttcaatcag catgtcggac aggcggtcca
     4741 gcccgctcca ggaagcccag tgcagcgcgg tccacccgtc cttgtccttc cggtcgattt
     4801 ccacgcgggg gtcttccaga agccggtcca gataaggggc ccgagtggct tccagcttca
     4861 gcgtggtctc aatgttgttg tagttggcgt agcataccca catgacagga gttctgccat
     4921 ggggatccgt catgttgaca aaagcctttc cttctgccgc agccagttct tccccttcct
     4981 tcagggtttg ttcgaattcc ttcattccct tgactcgggc ttccttctcc tcctgttcgg
     5041 aggaagcgtt gacttctttg gcctccgctt tcttcattac ggtgataagg tccagggccg
     5101 ggttgggatc tccggagaaa gatttggaga ccaacaggct gagaccgccg aggagggagc
     5161 tgaaagctac caggatgagg gaatccacaa tgatgttgcg cgtgtgctgg ttcatggtct
     5221 cgtaaggtca ggagttggct tccgtaagtt cttccgccgt cagggcggga tccgtttccg
     5281 cttcaatggc gctgatgggt ggcgatgccg cgtcttcctc gggatggtcc gaggtgatgg
     5341 tcctgcgggt catgataatg gcgatggggc tgattacggc catggccagg taaatggtcc
     5401 acatgaattc cggggagtcc cagaagccga tgtgcccggt atccatcttg gcgcagatgc
     5461 cgtcatagca gaggtaggcg acgagcaggc cgccgatgat gccattgatg ggtttggcga
     5521 tgaacatggg aagcgccgcc agagacatgt aggaagccac tttgtctttc ggagctaccc
     5581 gcgccacata ttcggagaag cggggagaga agagcagttc cccgaatgcg aataccaaga
     5641 tctgcgccac aatggctacc agatacgcct gggagcgcgt ttcgatgccg ggcaccaggt
     5701 agtaccattc aatgggaatg gccatgaaaa cgagagagaa agcggagatg gacatgcccg
     5761 caatcatgag gccaacggta gagaagcggc ccagcaccgg aatgagcagg atgaggccgg
     5821 agacgatgat ggccgggttg acggcattga tggagcctat ggcgaaatcg tcatatagcg
     5881 tccgcacgta gtatttgggc atcaccagga attgcagcgt gaaaacgatg cgcacgccca
     5941 tggtcagcac caggaagagg ataagtttgc ggaaggcgga ttccttccat acctccatga
     6001 agagctggag aggtctgcgg aggggggcat tgatgttcgc ctcccgttcg ctgggttccg
     6061 cgtagttgtc ttcattaata aaacgggtgg tgatgcagcc cagcagcacc atcagcgttc
     6121 cgaagttgat gatccagtac ccgccgtcca cggcgccgaa gtgcttgcgg aaaaattcaa
     6181 tcaggaacac gttcgcaatg acggcggaaa tgttcatcag caggtagtag atattgaagc
     6241 ccgtggaacg cgctttcagc ggggtgaagc gccggatgga agtctggatg acgggggaca
     6301 tgaacgccgt gccgaaggac ataatcagaa tgcccgccat gacggccagc cttccggagt
     6361 ccacggtcgg gctgaggtcc ggaccgaagc ccatgataag gcgcccggca atgaggatga
     6421 tgaagccgat taggtaagtg cgccgtatgc cgatgatgtc gcagatggaa cccaccgcga
     6481 agacgaaggc ggagatgaaa agggtgtaca ggcctaccca gagggcggcg tccgcatcgt
     6541 tgaagctaca atagtccgtc aggtagagga cgaataccgt gatcatggaa aaataggcca
     6601 ttccgtcgaa gaactggacg acgttggtaa gccagaaatc cttggatacg gttttcagta
     6661 ttttgaattc cgaaaaatat ttgaggatgg ggtttgcgga ctcattcatg gagagatgga
     6721 agcggtaagg aaaagttgac gacggtgaaa gagtgggtta cacgcatccc cgtttcaaga
     6781 tttttttctc ccgaaggccg gtttgaccgg aaaaatgcgc ttcaccgcca ggaaaccccc
     6841 tctatctggg caaaatacgc cggaagcctg ccacgtggca ccatgacagc gctttcaggc
     6901 attgttccac agccatggcg tccgaaggca gcacgggcca cagcccggct tccttcagcc
     6961 ctgatttggc tatttccgcc gctttccatt cgcggaactg ggtgggaagg gcggagaaag
     7021 tgaaaaaggc ccagcaggcc agcccgatgg ccagcgcgat ctggaatttc aacagtccgg
     7081 ccgccagcat tcccagcagg ataagagctg tgaaaaaggg aatcatcgtg gcgcgcgcag
     7141 aggcggtttt gcgggctttt gccgcctccg ggtcttcccg gaccaggctg ctcatggccc
     7201 aggtatgggc tacgcaggcc agccggcctg cggtcacctt gccttcccat gccggaggaa
     7261 ggctgatgaa tccgttttca tccactccgg gaaaccgggc tttctccagt ttccgctggc
     7321 gtttttccgc accgtccagg cggtggatta atcccgctgg gccggggaaa ccgggcagtt
     7381 cccgggattc gtaggtgcgc aggggaatga tgcctttggt gaaacgcagc gcggcaatga
     7441 caccgagcac gaccacggaa aggaaaatca ctactgcgaa tagcatagac ggctttttct
     7501 catatgcccg tgtgtccgtc aatctctccg gctggcatga agccgtcctt cccggccaac
     7561 agccgggtgt ggatgcggac atcgtttttt ccttcttttc ccgcccggaa cgcttggaaa
     7621 acggtgggat ggcgcttatg tcggaatgat ctgcggtttc gggtgttcct ctgggcgggc
     7681 acaacattcc ggcatggatt ttcttcatgt cggaggcgct ggatttgggt tgattttccg
     7741 gcgtttttca tgtaatggct gaccgcaatg gtccatattc gggaatgcat gaggaggtgg
     7801 ctgccgtttg tcataatggc ggcttccctg ggtgtgtctc ccgcccagga tgtggcgagc
     7861 cccgatgccg cagatctgga tttggacctg gtcaaactga aaagtggccc caagggcaga
     7921 cagagggagg ccatgaggaa ggtcctcctg cttgtgcatc tgaagcaaaa ggaaacgtgg
     7981 ctggcggaat ccacggggga tgaggccgtg catctggcgg cggagatttc cttccgccgg
     8041 tatttggctt cagtagatat ggacggatgc ccggaggaat tcaggcgcgc tttcctgcgg
     8101 tatgcaaagc tcctgaattc ctttgaagaa aaagatttga acagtgtcgc ggattttcct
     8161 gcggtccctt tgcaggctgc cggaagggaa ctgttccggg tgttgcttac ttaccgtctg
     8221 gaaccggagc atctgctgga ggaaatgaaa gcgctggtgg atgaggaaat tgaggggaaa
     8281 gaggagctgt ctgcaggaca gatgatggat attgtccggg gaatgcggga agccctgcaa
     8341 tccggaagcc gcccctttcc agcggaaagg cctccggaag acagggtttc cgggatgtag
     8401 cggcttttca tggatggcat gccggtaccg tgaggaatct tgtggaggca tgccggaagt
     8461 ccgctccggc tattccggaa ggaaaatgga ttaagacggc tgtacgtcag ggtttccagg
     8521 cggcggccat cagagtctgg aaatggggcg ggtccggctc cctgtccaca atatccgtgt
     8581 ggatgttccg gaatcccgct tccttcagca tgctttccag ggcggcgggc gtgaagccca
     8641 gccagcggtc cgcgtaaagt tcacgggctt cctcctgtcc atgctggagc agatccagca
     8701 ccaccaggca accgccggat ttcaggatat cccacgcagc ttccagtgct ttggacggtt
     8761 tttgcgcatg gtgcagggcc tggctgagca gggccagatc caccgtgccg ggtttaatgg
     8821 gcggcgcttc gatgtcgccc aggcggtatt ccagattgtc cagcccgtgt ttgcgcgcca
     8881 gctcctgccc cagctccacc atgctggggg agttgtccac ggcgatcacc tgtttggcgc
     8941 tgggggagat gagctgggaa acgaaacctt ccccggctcc caggtccgcc actacgtcat
     9001 agttcagaat gcggaggaga gctccggcca gcgctttcca ggaacggcct ggcgcgtagt
     9061 tcttgcccag acggcacacc acttcgtcaa aataggcctg ggtgcggttt ttgcgcttgt
     9121 ccaggataac ctgaagggct tttttgtccg tttccgcctc cggcaattcc tcgcaggctt
     9181 tcagggctac gttgtgcaga tcgcctgcca gggaaagagt gtaaaagacg tttttgccgg
     9241 aacgggcgtc ctccaccacc ccttcctgcc ggagctgggc cagctgggtg gagatgcggc
     9301 tttgccccat gccgagaatt tcctgaagtt ccgcgacgct cagggattct tcattcagaa
     9361 cgttgatgat gcgcaggcgg gtgggatctg ccaggagttt gagcgttttt agggttgact
     9421 tcatagcgga ataatatatc aacacatcga gatttatcga tatgagcaga aacacccctc
     9481 acatctttac gtcggaatcc gttggagaag gccatccgga caaggtggcc gactacattt
     9541 ccgactctat tttagacgcc tgcctggctc aagacaaaac tagccgcgtt gcatgtgaaa
     9601 cgttggtgaa gagcaatatg gtcatcattg ccggagagct gacgaccaag gcggtgatcg
     9661 atccggaaaa aatcgcccgc caggccattc gggagatcgg gtactgcaac aggcaggacg
     9721 acgacgtttt ccatgcggat accgtctttt tcacgaatct gctcacggaa cagtccccgg
     9781 atattgccca gggggtggac gcccgggagg cggagggcaa gggccatgcg gagcagggcg
     9841 ccggagacca gggcattatg ttcggcttcg caacgaatga gacgccggag ttgcttccgg
     9901 cccccatcgt gtttgcgcac aaactgctca ttgagctggc caggaggcgc aaacgcgggc
     9961 atgtggactg gctgcgtccg gactgcaagt cccaggtggc tgtggcctat gacgaagacg
    10021 ggcgtcccgc gcatattgaa aacgtagtta tttccaccca gcacacggaa gacgtggacc
    10081 atgacacgat ttactcatat tgcgtcaaac tcattaaaaa tgtgcttcct gcggagcttc
    10141 tggatgagag gacggaatat ttcatcaacc ccaccgggaa attcgtcgtg ggcggtcccc
    10201 acggggattc cggcctgacg ggccgcaaaa ttattgtgga cacgtacggc ggcatggggc
    10261 gccacggcgg cggagccttt tccggaaaag acccgtccaa ggtggaccgt tccgccgcgt
    10321 atatgtgccg ctgggttgcc aagcacattg tggctgccgg actggcggat aaatgtgaat
    10381 tgcaggtggc ttacgccatc ggctaccccg ctccggtttc catccgggtg gatacgttcg
    10441 gtaccgggaa ggtggaggaa atatccattg aacatgcgct ggaaaacatc ttttccttca
    10501 agcctgcgga tatggtggaa cagctgaacc tgcttcggcc tatttaccgg aagacgacgc
    10561 actacgggca tttcaccaat ccggagctgc cctgggaaca gctggacgaa acgcgcctgg
    10621 cctccctgaa acaacttctt cattaattcc tacgacaacg cttacagaca tgttttcaga
    10681 aacggaacaa tacaaggtgc gcgatatcgg cctggcggat ttcggacgca gggaactgga
    10741 tattgcggag catgagatgc ccggcctgat ggccgtgcgt gaaaagtacg ctgcagccag
    10801 gcccctggag ggcgtgcgca tcatgggctc cctgcacatg acggtgcaga cggccgtgct
    10861 gatagaaacg ctgacggctc tgggcgcttc cgtccgctgg gcgagctgca acattttctc
    10921 cacccaggac cacgccgccg ccgccatcgc caaatccggc acccccgtct tcgcctggaa
    10981 gggggaaacg ttgcgggaat actgggactg cacgtggaag gccatgaact ttccggacgg
    11041 gctggggccg cagctgattg tggacgacgg cggagacgcc accctgctta tccaccgcgg
    11101 gtatgaaatg gaggaaggct ccgactgggt ggataccccg tcggaatcgg aagaagagga
    11161 agtcatcaag gccctgttga aaaaaattgc cgcggcgact ccgggcattt tccatcgctg
    11221 gcttcccgag ctgaagggcg tttccgagga aacgaccacc ggcgtgcacc gcctgtacca
    11281 gatgcaggcc gccggccgtc tgctgattcc ggccatcaac gtaaacgatt ccgtaaccaa
    11341 gtccaagttt gacaacctgt acggttgccg tgagtccctt gtggacggca tcaagcgggc
    11401 cacggacgtg atggttgccg gaaaggtggc cgtcgtctgc ggctacgggg acgtaggcaa
    11461 gggctgcgcc cagtccctgc gcggcatggg agcccaggtg gttgtgacgg aaatagaccc
    11521 catctgcgct cttcaggcag ccatggaagg ttaccgcgta ctgacggtgg aagacacgct
    11581 gggctgggcg gatatttacg tgaccactac gggaaactgc aacatcatcc gcatcgaaca
    11641 catggaaaaa atgaaggacc aggtcattgt ctgcaacatc ggccacttcg ataatgaaat
    11701 ccaggtacac aagctccaga cgtatcccgg catcaggcat cttaacatca agccgcaggt
    11761 ggaccgctac acgttcccgt ccggcaacag cctgtatctt ctggcggaag gccgcctggt
    11821 caacctgggc tgcgctacgg gccatcccag cttcgtgatg tccaacagct tcgccaacca
    11881 ggtgctggcc cagctggaac tgtggaatac ccgtaaagac cgttccgtgg gcgtggaagt
    11941 gctgcccaag gtgctggatg aggaagtggc gcggctgcac ctcgccaaga tagggtgcaa
    12001 gctgactgtc ctgcgtccgg agcaggcgga ctacatcggc gttccggtgg aaggtcctta
    12061 caagccggac ttttacaggt attaaccttg tcaaggtttt cttttcctgg ccgttccgcg
    12121 caggcctgtc cggcggaacg gcttttttgt tttcccgatg atggagggaa gcaggataac
    12181 ggtacgttta tttttcttcc tgttttttat gcaggttccc tgctgttagt gaaaaaaagg
    12241 aagacttttt atccggtctt taatgttcaa atacctttgc agcaggcaaa acgggagcaa
    12301 tcttggaatg ctgaccagcc agagcctcgg cctgcattgc agaatgttcc aaaaccattt
    12361 gagtttggta agaaggctgt tatcgaatga agcatagggg aagagcagga gccttctctg
    12421 ttccgcctga atcagcctca tcaggcgatc ggcggcttgg tatgttattt ccccggactt
    12481 atgctttccg tatacctctt tcatgatcat tatccaggtt gttatcagga attccgatct
    12541 cttgatcagg attgtcctgg ggttcccgct cttaaaattg cagatattac tgttgtgtct
    12601 ccgatagtac gaacaccgtt tgccggatgc gaccatgcct gattttttca aataggcgcg
    12661 catggacagc attgtatcgt ccgcaatgcc ggggcctgaa ggcaaatatt cgaagtcgct
    12721 gaacaaagac ctgtggtaca tggcatttcc gccaaaacag ctgtaaacat gggaaatgtc
    12781 aaaaatacat tccctgtagg gtttgacgac gacggaatct gaatctggca acatgcgtcc
    12841 ataagcgggt tgtccatctt catcgatttc attataagaa tggcagtaaa ccagatgacc
    12901 gggatatttt tccgccagag atgccaacga ggagatcctg tcttcccggg cgatgtcatc
    12961 gccgtcaaac ttgataatcc attccccccg ggacaaatga acggcacatt gcaaattccc
    13021 tgcaattccc cggttgcttt cgttacggtg gagaataata cgccgtcctt ctccctcttt
    13081 ttccaccatt tcttccaggc attcaaaaga accgtcctga gaaaaatcgt ccgaaataat
    13141 tatttcccat tctcctggat aatcctgttt taaaatagca cggaactgtt ccttgataaa
    13201 acgtttctga ttgtaacaaa gaagaatggc tgaa
//
LOCUS       DAWEMX010000068        12848 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2048_length_12848_cov_9.316189, whole genome shotgun sequence.
ACCESSION   DAWEMX010000068 DAWEMX010000000
VERSION     DAWEMX010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12848
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_12848_cov_9.316189"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8465..8474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11621..11630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2722 a         3248 c         3727 g         3131 t
ORIGIN      
        1 aaagtgagga taaataattt tatggaagaa aaaaataaag ataatgaaac ttgtatatat
       61 tgtggtctat ctgatgaagt atttcgtatt tcaagaatac cagtatctag atggagaaaa
      121 ttcctatttt tatttgtgtc taaactattg aatgtaagag aagaatttct gtataggtgg
      181 cgatacaaag gcaagaaaat tgatacaaaa atggttgtga agtaaacttc acaaccatga
      241 aattcttaat taaaatgtag atttataatc gcttattaac ccaattccct tgttcctttg
      301 taaggagccc ttgattattt ttattcactt ctgatatgaa gagtttatca ttcttatcaa
      361 agtgatcttt taatcttttg gttatttgtt cagcatctga gtctgtataa acaagataat
      421 gtgatttcat aatcttgcag ttatcatacg cttcaattgc agcatataag tcctcatatt
      481 cttgtcctgg cgaattcaag tcataagaaa tactgtaaat tttgctcatt taattttcct
      541 cctttctact caataagaag tttgcgttga gaaacgacca tatggtctat caatttctca
      601 acctcatatc ttatatgggc aaatatcatg ccagttttta taaaattata taactcattt
      661 gtattctata gaaataaatt gttttccaac aatttaaata aataaaataa gtgtgtcaaa
      721 atgtcacaat attagacaaa ttgacgcagc taatgtgaca taatgaccgg gggcccacat
      781 atcaggcgac tttactcagc agaatccttc aggacatcat ccagaagact atgaacgtct
      841 atctgtaggc atttgcagat gtcaatgacc tcggtgaagg taagatacct ttctcctacc
      901 tcatatttgt tgacgaaggt atgaggagta ttcaggcgtt ctcctaagtc acgttgagtc
      961 aattttaact ccaaacgttt ttcttttagt atattacgga gatttttctc ttcattacta
     1021 ttcctgttcc tcatttagga acaataacag agaatgagat tgtaccaaat ttaggtacaa
     1081 aagtgtttac aagttatccg atattcggta ataggtcttc aatgttccta aaatgggaac
     1141 aaacttagat aagaactatg aagacattat tacttggaac agtcctagcg tccttggctt
     1201 tgcttacctc ctgcagcaag tcatcatcca atggagatgg aaacaattca gaagttccag
     1261 aaacattgga tggaacatcc ttggttggtg aattgtatgt agattatcag gaaggagatg
     1321 gttttgagtt ggaagccaaa gatcttacct tctcgggaaa tgtggtttca ttcaatacag
     1381 caacttatcc tggtactgat acattagtat gggcaaatgt tactggtacc tataccttcg
     1441 aaaaaacagg gattaatact gggaagcttt ctgtggaaag taatcctgtg agctataagt
     1501 tggatatgac atttaccaac aatcaagtac aaagctatgg aataatgatt gaaagtagta
     1561 cgagattaga atttcccgta aaaggaacat ttaataagag ataatttatt acaaatatat
     1621 tatgaaaacg ctgactagaa tacctagtca gcgtttttgt ttaagctgtt caccttcaga
     1681 caaaaaagaa atgacgaaat tttataaaat agtatggtta gtcaagtatg taggtcccaa
     1741 aaaaatcatt gtctgacacg ggaaaaacgg cagtttctgg aaaggggcgg cattcgggaa
     1801 agcatgcctc tgcgcgcagc gtttttccgt gaaagggtaa cgtttttcca tggaaggccg
     1861 ttcatttggc ttgcgtggcg gggggatgga gtgcaagctg tgcgcgttat gctcgacatc
     1921 cgtgtaatac gagaaaatcc ggaagaagtg aaagcgcggc tgaagccccg cagcggcgac
     1981 gcgtggaagc ttgttgatga cgttctggcc tgcgatgaag cgcgccgcaa tgccgagacg
     2041 gaaaagcagg ccctgcagtc cgagcgcaat gccacctcca agcagatcgg catgctcaag
     2101 aagaaagggg aagacacttc ctccattgaa gcgcgcgtgc gcgaaatcgg ggaccgcatc
     2161 cgggaactgg accgtgtcgc ggaggaaaat gcagcccggc tcacctccct gctgatgaac
     2221 attcccaacc tgccgcacct ggaatgcccc gtgggtgcgg atgaaacggc caacccggaa
     2281 atcaagcttt ggggagaaaa gcccgtcatt gaaaacccga aggaccatgt ggaactggcc
     2341 gcgaatctgg gcatgatttc ctttgaagac ggagcgcgga ttacgggctc cggttttgtg
     2401 gtgtaccgtg gggcgggcgc ccgtctggaa cgtgccctga tcaacttcct gctgaatacg
     2461 cagaccgtgg aaaacggtta tcaggaagtg ggcgttcctt ttgtggtgaa aagggagtgc
     2521 atggaaggca ccggccagct tcctaaattt gaggaagaca tgtacggcac ggaagaccag
     2581 cagatgttcc tgattcccac ggcggaagtg ccggtcacca acctgtaccg ggatactatt
     2641 ttacaggaaa gcgacctgcc catcaagatg gccgcgtata cgccctgctt ccgccgggaa
     2701 gccggaagcg ccggccgcat caaccgcggt atgatccgcg tccaccagtt tgacaaggtg
     2761 gaactggtgc agattttaaa gccggaagac tccttccggg agctggaagt gctccgcagc
     2821 catgcggaat ccatccttca aaaactgggg ctgcattacc gggtgattga gctctgcacg
     2881 ggagacctcg gtttttcctc cgccaagacg tatgatattg aagtatgggc tccgggacag
     2941 gggcagtatc tggaagtttc ttcctgttcc tgctttacag actaccaggc gcgccgcatg
     3001 cgcctgcgtt acaaggatgc cgacggcaag aaccagttct gccacaccct gaacggttcc
     3061 ggcacggctc tgccgcggct gtatgtggcc cttctggaaa cttaccagca gctggacggc
     3121 tccatccgga ttccggaggc gcttgtgccg tatttcggtg cggactgcat ccgtcctgaa
     3181 caggcttgaa cctgcgtttt tcccgtccca accctgaatc agcccatgcc tgacgagggc
     3241 cctctggaac attacctgac tgtttcccat gagccggaac cggacattcc ggaaccggct
     3301 ccggaacgca gtcctggacc gtggcgggaa aggggcatca tgctgggcct gggctttctg
     3361 ctggggtgca tcggggctta tgcgctggtt tactcggaaa tgtccggcgg catgcggtcg
     3421 ctgaagcagt cgctttcaga agaacgcgac ttgaacgcgt ccctgcagaa agaacgtgcc
     3481 catctgcggg aaacgttgga acaggcacgc atggacgctc tgctggagga acgctccccc
     3541 ctttccgtga cggtcggcgg tattcccaag acaagccgca ggcccgttcc ccagcctccg
     3601 ccgcttacgc cattatcctc cggaggactg gcatccccgt cttccgcgcc ggttcccggt
     3661 tctctggatg accgtctggt caatccgggg ccggaggact ggaaggagcg ttaccagtac
     3721 gccgctttga aatataacca ggtggtggag gattacaaca ggctgaagaa actttacatg
     3781 aacctggtgg gccggagcgg tgcagccggc gggctggtga cgggggccca gttttacaaa
     3841 gctctgcgga aagatacccg tgcggaggcg cgcaagctgg atgaacgcta catgaaggga
     3901 gggatgcggg gcagcgagct tcgggaactg gaacgcagcc gggacgagat gaggcaccgg
     3961 gactggtggt tgaaaaaaat ggcacgccag ttccagattg attagatacg tgggtgttgc
     4021 cggcgtgaag tattgttttt gtgaagcgcc ccttgctgaa attcctgttg catggaatct
     4081 ttttgcagag agccgcacgg tcacaaaaag aaatcccttt cccaaagagc cgggatgaaa
     4141 aaacaacatc cgaatacgca gataccggta tctttttttt gcgggatgct ccttggatat
     4201 tgaaaatcag ggaacaaagt agcccatgcc cgcttattcc tgtgtccggt tcgcaccgga
     4261 ttccgcagac tgctgatctc tgaggcggaa aaactatccg tcagcacagt tccgctgcga
     4321 tggcgtccac gagcaggcgt ccgcggcctt tcaggatgag cctttgttcc ggggaaatgt
     4381 ccgccaggcc cagttcccgg tattgttcca gcagaggacg ggattccggc agaatgtatt
     4441 tcagcggcaa tccttcatcc gtccggagca tcagggcaat gcgttccagg cggtagtctt
     4501 cctcggtgac gggttcctgt tcggaaaggg ggaggccgtt ttccagcgtg ctgcgtatgt
     4561 aggcgtccgt atcccgcgtg ttggagtacc gtatgccgtt gatggtgctg accgcgccag
     4621 gccctatgcc tacatagtcc tctcccttcc agtaggccat gttgtggggg gagatgcagc
     4681 cctcccgggc gtagttggag gtttcatagt ggcgcaagcc tgccgcctcc agcatgtgtt
     4741 ccgccagttc aaaaaaggcg gcgtcttcgt ctggatcctg ccttttctcc ccccgcagca
     4801 ggctccggaa gaattccgtg tcttcttcat aggtgaggtt gtaggcggag atatggtccg
     4861 ttccggcgcg gacagcttcc ttcagggtgg tttcccatat ggagagggtt tgccccggaa
     4921 tggcgaacat gagatccatg ttgatatggg gcattcccgc atttttcagc atttccacgg
     4981 agtgcagagc ctgggccggg gtatgttcgc gccccagcag atgcaggatg ccggaatcca
     5041 gggactgcac gcccagggag acgcgtgtca tgcccaggct gcgccagaag cgcacttttt
     5101 tttccgtaaa ggtggcgggg ttggcctcaa aggagaattc gtccagtttg tccacgggca
     5161 cgagacggtc cagggtttcc atgagacgcc ccagatgcgt gtccgagagc atggagggcg
     5221 ttcccccgcc aaagtaaagc gtggccgttt cccctccgcg gttcgcggcc agagcggctg
     5281 cgcgggattc cgcctccctg cccagagcac ggatgaatga tttcatgtcc gtggaggccg
     5341 gcgtgtgctt gaagaaagcg cagtacgggc aaatacggtg gcagaaggga atgtggacgt
     5401 agaggtgcac ggaagcgggg aatcagcgtc tccggcggcg tccggaggat ccatttccat
     5461 cgcctttctt gtcctgggaa agctccagaa ttctgtcgga agcctctttc atttccgact
     5521 tgacgcggga tttttccgaa tcgtcagcca gagagatgta gtttttcgtc agggcgtcgt
     5581 agaaggggtg gattttcgtt tccttcttgt ttttgggctt gtatttgaac ccgtccggac
     5641 aggtgtccag catgtttttc atccagggga ccagttcttc cttgggcagg gcctggcaca
     5701 gggcggccag ggaaaaggac atttcctcct gatccacatc cttgggctcc ttcagggcgt
     5761 tgctgtcgtc ccggtagaaa ttgagggctt tcttgaatcc cctatctaca gttcttttga
     5821 gcctgtccgt gccctgggcc ttggcatacg ctatctgata ggcggcctga ggctccgggt
     5881 aggagttgcg tttggattca gacttgagcc aggacatgag ttcggcccac tggaaggtct
     5941 tatagtcata ttcctgtttc agggcatttt ccagaatcac ggagcagagc acattgacgt
     6001 attccctggc atggttggcc agcatgtcct tgccgttttt gtcgccgcgg cctacggagg
     6061 aaacgtattt gcgcgttttc cagagttttc tgatgcccat gccgtccggc agggcctccc
     6121 tcacttcatc caccatcttg gtggctccca tgaggtcctg gggttcctgc ttgaagagcg
     6181 tgtaccaggc ttccgtgtag aacgggttgg ccttgagcac ggtcctcatg gcggtctgcg
     6241 tgaattcctt ggggagcgtg gcggaagggt cggccgtctc ttccgtggag gagccggtgg
     6301 ccgccagttt gtaaatgttc atggcgaatc ggctgtcgat gaacttttca tagtcgatat
     6361 tcatggacag ggccagtccg gattggtatt ccgcattgcc ggtgcgcggt gcgtcggaat
     6421 cccggaagta ccagaatccc gtggtggctt tcagggtgtc cacgctttgc cccacgctca
     6481 gccagcagcc gttgccggtg aaatgggtgg tcatcaggtt gccgtggccg ggctgcccgg
     6541 cgggggcggc gggaatgccg cgcgcgatct gggaattgcg ggagataagg gacatggtgc
     6601 cgcacacgcc gccttcatgg agcttgcgtt cattggattc cctggaccat tccggatccg
     6661 ggtcaaagct gaacaggatg ggcggttcct tgtcgtcatc cgcccggtag gggccgtaca
     6721 gccacaggcg ggtcgggccc cgctggccca tgtagcgttc ccacacgctg tcgcactcac
     6781 gcagagggcg cgtttccgaa agggccatca tggccggcca gggcgttcct tccagggaaa
     6841 cgccggtcca ttccacacgc ttgcggtcca cgtcgcgcaa atcaccgcaa tgttcatatt
     6901 tatccaccag gtaggaaaag aattccaccg gagtggggaa gggatccctc ttgcgcctca
     6961 gctggccgtg gcggtacatg tattcgtgca gaaagtcggc caggttgtct tccgtaatgg
     7021 cggagtcccc aatttccgag acggtggggt atttcctgag ccaggcctgc tttggggtga
     7081 tttttttgcg gtccaggtag gcttccacct ttttgtagca ttcctcatcc acggtgcggg
     7141 gggcgttccc ataccagttg acgggcggtt tcccggcgca gtacaggtcc gctggcggag
     7201 gcatgacccc ggaggatctc aggtcattca gcttgcccag ggcatcaacg tagcggccct
     7261 gatgggtaag gtcgaaggct cccatgccga attcgcggcg tcctactgcg aacgccagaa
     7321 cgagctggtc atatttttcc gtcgtttcca gccccagttc atcaatcatg aaagccacat
     7381 tggtggcgac gctggggtcc ggagggaaga acatggcgaa gacttcacga ttgtttttcg
     7441 tgtttttcag ccatttttca aaatccggag aaaaattgcc tccgcgtttt ttcacgtaat
     7501 cccgtacgta ggcttccgcg taatggggat atttattcaa cagtttttca gaaaatttcc
     7561 ccttggacgc gatagcttcc tcccgcagag ccttttccat ggcaatgact tccggggagg
     7621 gggaaaggtc gggggcaggc ggttccggtt caggctccgg ttccggttcc ggttcgggag
     7681 cggccaccac gggttccggt tcgggggcaa ctaccacggg ctccggtttt ggcgtagcgg
     7741 gcttgggttt gggcttgggc ggagccggta cgggcacggt aacagcttta gcgacgggtt
     7801 cttctttatc cttgagtatc tggtagccca gaaatccgaa gagggcgagc atcccggtca
     7861 caatgaccgt gatgaccgga aatcctccgg agtgctttac ccgcggcagc ggcgttccgg
     7921 ggatggcggc gccgactacg ggcttccgca gttgcggttg ctgcggtttt tttgcggggc
     7981 ggagagcctg ggcatgcgct acagccggct ggggttgtgc cggagaaagc gggtaaagga
     8041 agagcacggt gccgaatccc ctcagcttgc cttctgcatt ggctgcgtat tgggtttcaa
     8101 aagcttcccg ggcctgggcg aattgggatt cattttccgg agagacagcc agttctccgc
     8161 tggccaggtc gcgcgtaacg aggccccggg ggcagaattc gcgtatcagg acggcatggc
     8221 cttccgggga aagagcctgg tacagatgat agtccggacc gctttctaca agcgccgtga
     8281 ttttgtagcc gcctatgttg aaatggggcg gcagggcagg gggaggaggg attgtcatga
     8341 cgaatcaaga ggtcaaagat cccgtatatt caaaaaggtt cggagccgtt tgtcaatgcc
     8401 ggagtgagga agctgcgcca tgaaacggga gcagttatca gcacggaacg cttccggacg
     8461 gcggnnnnnn nnnntgcggc gcgagaccgc gcaggaagcg gctaacggat ggtgaccttg
     8521 gtgccgatgg gggtgtgttc aaagaatatc ttagccatgt cctccggcag gcgtacgcag
     8581 ccgtggctga ccggatatcc ggtgacaaaa cccacatgca tgccgtagcc tcccgtaatc
     8641 cgcatccagt agggcatggg ggcggggtca aagtaggttc ctgccggcgc tccgccagcc
     8701 ctggcgttgt aatccccgtt gacgacggct ccggtggatt tgctgcgcag aacgccgtat
     8761 gtgccggatt tgtagttggc gtctttctgg atgattttgt atgttcccct gggagtgccg
     8821 tagccttcct tgccggaaga catgggggaa acgcccacga gcgtggttcc cacgtagtag
     8881 aagactttct gctgcttgag gtcaatgatg atgtgctttt ccccctgggt gcccgccggc
     8941 aggtcccaat atccctggcc cagacgggct tccggaggca ggtccgtgtt gacgcctgag
     9001 gtggcgccca ccccggacag ataatcgtcc cgtccggaca ttccctgaat ttttccggtg
     9061 atttggtcaa acagcggggg cctctcgtac gatgtgcagg aaacaagagc ggaactgata
     9121 gaaactaacg ccagtatgcc ggatttcatg gtaacgggaa aatgtcaggc aggttatggg
     9181 gcctgtcctc ctttttgtca agatttgcat gctgttatcc gttgcttatt ccgggggtat
     9241 ctgttataca cggcgaacca tttcattcat ccctattttc atgagcgaac aatacgatca
     9301 tcttaaaagc attcttttgg aaaaatccgt gcgtacggga acttttaccc tggcttccgg
     9361 caaggtgtcc gatttgtatg tggactgccg catgacagcc ctggaccccg taggagccac
     9421 cctggtgggc gctctgggct ggaagctggt gaaggaggat attttgcccc gtttcccgga
     9481 ggtggcggcc atcggcggca tgaccatggg ggcggaccct atttccctgg ccgtcggcat
     9541 gaccagcgct ttggaagggg cggaaaagac gcttcaggtg ttcaccgtgc gcaaagaacc
     9601 gaaggatcat ggacgcggcc gccgcattga aggcaatttc caggccgggg ataccgtggt
     9661 cgtggtggat gacgttatca cgacgggcgg ctccacgctt aaggctattg acgccattga
     9721 ggcggaaggg ggcagggttg ccgccgccct ggtgctgctg gaccgagagg aaggcggccg
     9781 ccaggcgatt gaggaacgcg gcattcccgt ttacccgctg tacaccagga cttccttgtt
     9841 gggccattaa tctttttcca tccatgctcc ctgccctgat cggcataagc ggccatgaag
     9901 tgggcgcgga ggaagaggct gccatccggc gtttgcagcc ggccgggttc attctgtttt
     9961 ccaggaatat tgattccgtg gagcaggtgc gcggcctgac ggagtcgctg cggaaacttt
    10021 gcctccacca tcctgtcatt gccgtggatc aggagggggg gcgggtggtt cgcaccgctt
    10081 ccctgggctt gaatttgccc tccccggctt cgctggcccg gctcggttcg gttggcggca
    10141 tcgtggaact gggcgcggtg acggctctgg ctctccgcta cctgggggtg aacctgaatt
    10201 ttgccccggt gctggatatt tgccatgatc cgtccgcagc caacgcgctg cccggccgct
    10261 gctggggaga caatgcgcag gacgttattt cacgcggcgg cgtttatgcc tccaacctgc
    10321 gccggggagg cgtgcagagc tgcggcaaac attttcccgg catggggcgc gccctggcgg
    10381 atccccattt cagccttccc gtgatcggcc tggatgaacg ggagctgttc aagacggatc
    10441 tgctgccttt tctggctctg tgtccggcat tgtcctccat catgtccgcc catatcatgc
    10501 tgcctcagat tgatcccgat tatcccgcca ccttgtctga acgggtgatc agggggcttc
    10561 tgcgtgaccg cctcggtttc cggggtgtgg tgtttacgga tgatttgtgc atgggggcga
    10621 ttacgacgca gtattcaccg gatgacgccg ccttcctgtc cctgaaggcc ggatgcgatc
    10681 ttcctctgat ttgccatgac cctcttccct ggctggatgg gttggcctcc cgcctggaaa
    10741 gtttgaacgc ctatgaccgg gaggattctt tcaaacgggt ggaaaagctg agcgactccc
    10801 tgtgtttccc gtttccggaa aaggcttccc tgtgggattc ctgcctccgc cgtgcggagg
    10861 ctctatgccg cctggaagag gacggaggag aaaaactgcc ttcatcccca gtccagaaat
    10921 attgaggcag gtgccaatga cggtttttgc ctgagaatat gccgggaatg tcccggaaga
    10981 tttattcccg cggcagggag gaacaaaagg aaaaggtgtg tgggcaatca gtttcttttg
    11041 cggcgagccg ccagcaaggc cattcccaac aggcccagcg aagccacagc gggttccgga
    11101 acgaggtggt tgtagatact tgaggaatag actttgacat aatcgatgtc gaatgcggct
    11161 ccggaattgg ccagcgtatt ttggtcgatt cccttattga ctcctgcgtt ttccacccag
    11221 ttgcctccaa tctgctggtc gatgatcagg tagaattcgt tcccgtcctt gtcaaagggc
    11281 cagtttgcgt tattgggctt tttgaatgaa ccggttgctt ttccatccac ataaaaggtg
    11341 atgccttctt ccgtccattc cgtcccgtag gtatgccagt ttgatttttc agcaccatcg
    11401 ttccaaccgg gggtgacccc ctgggagtta tcctggttgg gaacgccgtt ttgcgaccag
    11461 tggattgtct ggtaaacacg gccttcgtaa ttaaggtgtt ccatgatgtc aatttcccct
    11521 ccgacaggcc agccgatgct gtcggatttg ggcatcatcc agatggccgg ccagacgccc
    11581 tgcacgcagt cgaatctggc gcggacttct acgtagccat nnnnnnnnnn atattggaag
    11641 gagaaggttt tcagggaata tacccctccg caggcgtatg tcttggctgg ggcggtctgg
    11701 ttggtttgag tggtgtagtc cccgtatttt ccccacaggg tcatggcgga gttgccgtcc
    11761 ttttcccgga attccacaag gctttcgtcc cgggattggt acttgcgcca gtccggggca
    11821 ttatatccta cgtaatcaat gcggctccag ttgtgtgcgt tcagcgatga gccgtcaaaa
    11881 ttgtcttcaa aaacgagctc ccagttcctg tcagaagccc aaggggttgc cgccatgacg
    11941 gaagatcctg aaagtgagag aaaaaggata ggaaaaagga gcgttgtcct gatgaacata
    12001 atgaggaaat ataggggagg cgcggcttgg tgtcaataga caagaaattg gatggccgcc
    12061 ccggcatgga gcagggaatt tcctccctcc ttaaaatggt atatccggtt tttccagcag
    12121 ggacacggag attggcgccc ctgccgggat ttcctcatca caagtttttt tattattcct
    12181 ggccgcaggt gaaagccgtt taagactgga gagccatatt tctgacggcg tttgccttgt
    12241 acacgtgcag ttccgcaagg cggacttttc cgcccttctg gaagagcttg atgcgcagcc
    12301 tgttggtggt gacagggtca aacgtccgca tgcatttgat aaatccattg cccggaagct
    12361 ggagcatgcg cagtacttcc ggttcattcg cgtacatggc tttctgagca tcgctgacac
    12421 cggcagtaga ggcccccagg gagccccagt gaataggaat ccacttgccg cctttgttgg
    12481 cttcaatgac taaacggttg ttgccctttt caatcttgat gtccacggcg cgcagggcgg
    12541 caggggattt cagggtgagt tccagcacca ggggctcgtc ttccgagagg gacgcgtcca
    12601 gttcttcaac ggtggtagcg tccctgtcca gtagtttagc cgtatccaca cggcctccgg
    12661 tagaggcttt cagcttatag tccgcgactt cctccgtgga actgcccagc agtttcagtt
    12721 cacgcacacg gcattctagg ccgtgccagt ccgtggaaac gcggaacagg cagcgggaac
    12781 cttgagtcag gcaggagtca ttcggggaaa cccaggttgt ttccttcttt ttgtccgttt
    12841 tgtcgtac
//
LOCUS       DAWEMX010000069        12729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2075_length_12729_cov_11.855531, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000069 DAWEMX010000000
VERSION     DAWEMX010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2075_length_12729_cov_11.855531"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5050..5059
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3045 a         3873 c         3192 g         2609 t
ORIGIN      
        1 acaaggacca agacaaggac caagacaagg accaagacaa ggaccaagac aaggaccaag
       61 acaaggataa cgccgaaacc aaggaaggca agagggacga aaatgaaatg aaaaacgccc
      121 ggcttccctc ctctccggaa aaagacaggc tgccagaaac gtccggaatg gaaaaaccgc
      181 agccccagcc ggaagataac aagcccgttc cggcagccgc acaaaaggaa agcagggagg
      241 aaaaggaacg cagggaagca agagccatcc tgatggaacg cagggacatt gaacccggct
      301 gccccgttcc ccagcgttct ccggaaattc ctccggataa ggattactaa cccgttccgc
      361 ccgcatccgc gaagccaccc ctttaaacat atcccgcaaa acaagatgaa aaaactgctc
      421 gccattcttt cgcttttctt ctgcatgact gtcacgctta tggctcagca ggtggttacc
      481 acctggctgt ccgatgccgt tgtgccggga gaacaaaccc gtctcttcat catcctgaca
      541 gacggaagca tcgcccgcca ggacccgctt ccccgggtaa agggcgccag cctgcgctgg
      601 gtcagccagg gcaactacat ccgctggccg gggtctccca accaatccgc cttcctgatg
      661 gccattgaag taacgccgga cgaagtgggc accgtggaaa tcccttccct gaccctgcag
      721 gccaacaatg gccgcactta taccacgctt ccccagacgc tgactgtcta cccttacagc
      781 gccatcaaat ggaatacgct gaacctgaac ggcacagcag tcccgtacgg catgctgtgg
      841 catgtggaca accagaaacc ctatgtccac cagccggacc gctgcgaact gaaaatttac
      901 gccccggaat acattctgga atacacggcg cccgccatca ccacctccaa cctggctacc
      961 tggcgctttg aacccacgct ggtggaactg ctccgcggcc agcctcgcgg ttctgtactt
     1021 tacaaaggcg tcaactggaa tgtcatgacc ttccagagca ccctgttccc gctgcgcgcc
     1081 ggggaggtaa cggcgtccgg cactgtcacg gcacgcgccg ccctgccgga ggcggaccct
     1141 ctcatcgcca acttcatccg ttcggaagta ctggtggaaa tggccattcc ggaagtgaaa
     1201 atcacagccc aggaactccc cccgggagct ccctcttcct tcaccaatgc cgtagggaat
     1261 ttccgcatct cctccaccac ggatgcgcgg gatctcagtg ccaatgaacc cattactgtt
     1321 aaaatcaggg taacaggaac aggcaatatc cacagtatcg cctgcccgac cctcacagac
     1381 gcctccaact ggaaactcta tcctcccaac aagctggacc cgggccagaa tacgcgctcc
     1441 atccagggaa ccgtggaatt tcagcaaatg atgcgtccgg ttgcccaaac ggatgccatc
     1501 cctccctttg aactcacttt ttttgacccc agcacgcaac agtacaaaac cgtaaccaca
     1561 gcccccattc ccctggaatg gaaggcctca gccattactg gaacggcggc cggaggtgcc
     1621 gctcccgcca cgcctccccc ggcaggcacc attccggtag ctgaaatgac ggatatttac
     1681 gggctcatgc ccgccaccgt catgaacgcc cttaccgccc cggctcactg ggggtggtat
     1741 ttcctggcct acatccccgc cgtcatcctg ctgggaatgg ctctgatccg ctacatccgg
     1801 aaaatccgga aggaaagctt cacggccagg gaacgcatga gagccttccg caggctcgct
     1861 tccactccgc gccaggcaag ctccacggaa ttcctgcgtg ccgccggcaa ctttattgaa
     1921 tcctatattc caccttccaa gcagaatgaa aaaatgaaag atatccttca actacgggac
     1981 gacagggcat tcaagccttc ggaatcttcg gaagaactgc cgcacgcgga acgccagcac
     2041 atgctccaag ctattaaaaa agccatcgcc aagcttcccg ttctcatgat ggccgccgta
     2101 ctggccatgt ccctggcagg agcaaccgga aacgcgtcgg aaacgaacaa cctgggtgta
     2161 aaagcctatg aacagggaga gtacgccaaa gccattgact atttcagcaa ggaaagcggc
     2221 aatatgaacc tttccaaggc agaacgctcc tacgcctgtt tcggcatcgg caattcctat
     2281 taccgcatga acaaacccgg tctggccgct ctcaattacc gccgtgctct ggaactctcc
     2341 ccgtacttca cggaagcgga atacaacctg caattcatcg aacgcaagga gggcgccatc
     2401 ctgcccgcca acaccacgga aaaccagtgg ctcacttaca tccgccatga tacgctcggc
     2461 cccatctcca tcctgtccgg agccgtcatg ctcaccttcc tggccctgct gaccgttacc
     2521 cgcagacacg gggccctgct gaccatcctg gccaccctta gcggactggt cacagtagcg
     2581 gctgtcatga actacatcat gtacccggaa accccggaat ccattccggc ggatcggttg
     2641 ctggtcgtta cccaaaaaac gcccggcaga catgcggcgg acatgaacag cccggcgctc
     2701 atcacccttc cggaatccac cccgctcatc ctgcgcgcgg aacgcggctc ctggtactac
     2761 gccagcacct tccagaacac gcccgtctgg atacccagga cggacgctca gccgctgtag
     2821 cgctttatta tcccgccgcc tcctctggag ccgcctctcc ggaatgccgt attcccggaa
     2881 gggcggcttt ctttcagccg cattcaatct tcttccctac tgaagaagcc ggaaccggaa
     2941 aacggcacat cctctgttca aggggaaaaa ccgggaagtc cccttccgga aacgtgtcag
     3001 aagattcccc ggccgcatga acaaaaagcc aataccctac ccacaaggcg tgcgtgtatt
     3061 gaaattaccc gctggcgccc tttcatcccg gacagatgaa aggcttgaaa caattctgat
     3121 gcggcaggaa aatccgcgcg tcccgcttca ccccaactgc cggacggcat cacggtttac
     3181 caggaatcca gatgcacctt tctgaccggg ggcggaaaag ccccatccag caaatccaca
     3241 tcttccgcag tcagcctgat ggacaaactc cggtaatttt cctgcacatg catgacggaa
     3301 ccggctttgg ggatcgcaat cattccaggg cagcgcaaaa tccaggccag ggcaatctgc
     3361 accggggtgg cctcatgctt ctgcgcaatc tccatcagca ccggattacg ggaaagccgc
     3421 ccttcatccg caggcgaata ggccatcacg ggaaggtggc gttcccggca ccacggcagc
     3481 aaatcgtact ccacacctcg atgggccaga ttataaagaa tctggttggc ggcgcaggaa
     3541 actcctcccg ggacggcgta aaaccgctcc atttccggaa catccatatt gctgacgccc
     3601 catgctttta ttttgccctc ctgctgcagc tcaatcattg aagcaaccgt atcttcaatg
     3661 ggatggggac ctccccagtg aagcagaaac aaatccacgt aatccgtctt cagcctgttc
     3721 aggctccgtt cgcaggccgc tttggttcct gtcctgctgg cgttgccggg caacaccttg
     3781 gtaaccagga aaacacggtc gcgcaaaccg cggattgccg ctcccaccat ttcctcgttg
     3841 ccatacatct ccgccgtatc cactacgctc atgcccagct caatgccggc gcgcaaagca
     3901 tctatttcat cggcttccct caacgctgac tttcccattt tccaggttcc ctgccccagg
     3961 gcgcaaacct tccgatggtc cggaaaaatt actgaatgtt ccatcattaa tatcttcttt
     4021 ctacttgatc gcatcattcc atgccgtaca aacctttgac agcagaggat aaaaaaaagc
     4081 tcaaaaatca ggacgttttt cctgaaccgc ctcacccgcc attttccctc ttttgctggc
     4141 gggacgtcaa taactctcat tgaattttca ttccatatgc ttccagtaaa catataagaa
     4201 atatccattc ccagccgtgc atagcagcat caaaaaaaac gcacagacct ccagccatac
     4261 cgactccgga acaacgggct ctcttctgga aaaatcacgc ccgatatcgg tttttcagcc
     4321 cccttttttc actgctgttt tgaaaataaa aagccgttcc gctgtcaacg gaacggctct
     4381 gaaggaattt cggcatcaaa gccgctgaaa aacatcaatg tatgcgggca ataagccaat
     4441 gcagcaggtc ttcaattgca acacgttcct gtttcatgga gtcgcggtgg cgaatggtca
     4501 ccgtcccctt gagcgaagca tcattttccc cgagagtttc aaagtccacg gttatgcaga
     4561 aaggagtacc cacttcatcc tgacgacggt aacggcggcc gacggctccc gtttcatcat
     4621 aaaagacgga catccacggc tgaagcgtct tttcaatttc ccgggcaata cgcacctgtt
     4681 ccccgttttt cttaagcaga gggaagatgg ccgccttgat cggagccatg cggggcacaa
     4741 agcgcaacac cgtgcggacg tcttttttac ctttgtcgtc caccagttcc tcttcatcgt
     4801 aagcttcgca gataatggcc agaatggtgc ggtcacaacc ggcagaaggc tccaccacat
     4861 ggggaatgaa tttctcccgc gtctcttcgt caaagtattc cagaggacgg ccggaacctt
     4921 tctgatggca gctcaggtca taatccgtcc ggtaggcgat gccctccagt tcctggatac
     4981 cgaaggggaa ttcatactcc aggtcatagg ttttcttgga gtagaaagcg cgttcgccgt
     5041 ccggcacgtn nnnnnnnnng ttcgcgcgga acccccactt cgtcatagaa gcaaagacgg
     5101 tgttccagcc attcatccac caggcgcagc ccgtcttccg gccggcagaa atattcgatt
     5161 tccatttgtt cgaattcgcg ggaacggaaa gtgaagttcc gcgggttgat ctcgttgcgg
     5221 aaagattttc cgatctgggc gattccgaaa ggcagtttga cgcgggaact gtccagaatg
     5281 ttcttatact ggcagaaaat ggactgggcc gtttccgggc gcagccagcc cgtagcgttg
     5341 ggatcgtttt catcagaggt agctccaatg gtcgtcttga acatgaggtt gaactcacgg
     5401 gcttccgtca gcagagaacc gttagcagga ttgtacatgg tggaatccgt tacggtttcc
     5461 atgcactcgc ctttcagctc catatccttg taagaggcat ccttgccgac cagctgggca
     5521 taatattgag ccgcggtctt acgggccttg tccgcctgtt tttcagattc caccagaacg
     5581 gagtacttct tttcaatatt ccaggaaccg tcttttttgg cggcccctgt atagaaaaac
     5641 cccatgccgc tctgggccgg gacctggtcc gcacgcagac gctccttggt caacaggcaa
     5701 tcgcacatcg ggtcggagaa tccgcccaca tggccggaag ccaccagtac ggagttatgc
     5761 gtcaggatgg aaccgtccat gccgaccaca tcctcacgtt cctgcacatt tttacgccac
     5821 cagtattctt tcagattgcg cttgagctcc acccccatgg gaccatagtc ccagcaaccg
     5881 ttcagaccgc cgtaaatttc agctgactgg aagatgaatc ccctcctttt acagaggctc
     5941 acgatttttt ccattcgggc ggggtctgtg ttggttctgt ctgccatgat aagttatata
     6001 attgggttgg ctatgcgggt tgtattgatg cccgcaatca gcattcagta aaagcaaaaa
     6061 gcgtgtcttc tcaagaattc caaggaaggg ggacaccctt gagcagctgg gagccatacc
     6121 ccgcttccac cagcgtgcgg aatgccgcca tcgcctccgg agtcacaggc tgttccgtgg
     6181 aaaatccctg tttaggatat tccagtatcc tttgaaagtc ccccaccatg gcggaaacaa
     6241 ccagatcctg cgggagctcc ctggtcagat attccctgaa cgagaaagga ggggagctga
     6301 ccgccacccg tacttccggc cactgggcct ggagcgtagc cagcgtgcgc cgttccatat
     6361 agggcttttg aagggcgatg atgctggatg gttccggaat gccggctttt tccaggacag
     6421 cgcgggaaaa gcggacattt tctcccgtat tggtggattt gttttcaatg agaatgcaat
     6481 cttccggcac tcccttcttc atagccgcct ctgcgaaaag ttccgcttcc ggtacggccc
     6541 attcccctgt aaaacgcccc attccgccgg aaaacagcaa caggggagcc agctttcggg
     6601 cataaagctc cgccgcatat tccgccaccc ttatgtcatt gctgccaaga atgaatatga
     6661 gttccgccgg acgcagttcc tgcttcacgt gatggtaatc ccataaaatt gctatggctt
     6721 cctctggagt catcggcttt tatgtactta agaaaatcct ctctgtaaac gccggattca
     6781 ccataatccg tatttactcc attccagagc ggcccatccc agcaacgcat tcgccgccgc
     6841 atgcatcatg atcacagcgc acaggttttt ccgccagatg gcaagcagat acgccaggga
     6901 accgtaaacg aatgcgccgc aatagtccac aggctggtgt accgccatga aacagagggt
     6961 agtcacccag tatgccttcc agctatgggt gccgaacggc accttccagg gatggtccgg
     7021 gttcaccatc aggcgcatca gataaccgcg ccagaaaagt tcctccacca gagccaccac
     7081 gaccacggca cggaagaagc gcagcaccag agcccccagc cagctccagg aacccgcaag
     7141 aaagacgacg cccggatcaa acccatccgg gcgcgcgtca attcccagca gataccggta
     7201 ccagggccag gagggatgcc caaaccaggc atccgcccct ccgggcaggc ggtccgctat
     7261 catggtcggg gccagccagc acaggatacc cagcacgccg aacaggattc cccaggcggc
     7321 gggtttccac cgccaatccc aatcaatggc ctttctccac cggaaaacca gggcaaagca
     7381 aacgattgcc tgaacgggat acagccattg ttccggcgcc cttctccacc agggtgcggc
     7441 cgggtggtcc cattccagca agggaacgcc aaactgccac accagggtaa agcccatgaa
     7501 taccgcaaac ggcgccacat acgcctttgt tttcagcgcc tccgtcacgg agttcatgaa
     7561 cactgccggg tcaggaaaga gtttccacga aatgttccat gcggttgcag gcttccttga
     7621 tctggtcaaa ggcggtcgca tagcaacagc gcaggaagcc ttctccgctt gcgccgaaag
     7681 cggtccccgg tacggcggcc acctgctttt ccatcagcag ccgggtggca aactccttgc
     7741 tggtcaggcc aaatttggaa atgtccggga agacatagaa cgcgccgccg ggcaggtggc
     7801 agtccatgcc gatttcatta aggcgcttca ccaggtaatc ccggcgctgg cggtagcttt
     7861 cccgcatctt caacatggcg gatgtcccgt tttccaatgc ctcaatggcc gcctcctggg
     7921 aagtaatggg ggcgcagagc atggaatact gatgaatttt catcatggcg gaaataagcg
     7981 gttccggacc gcaggcatac cccagccgga accccgtcat ggcgaatgcc ttggaaaatc
     8041 cgtgcagcag gagcgtgcgc tccttcatcc ccggcagaga agctatggaa acatgcggct
     8101 tgccgtcata acgcagttca ctgtaaattt catccgtcag tacgatgagg tcgtgcctga
     8161 tgcagatgcg ggcgatttcc tgaagggttt ccacaggggc caccgctccg gtcggattcg
     8221 tcgggaagtt gagcatcagc accttggtcc gcggtgtaat ggacgcttcc agcacctccg
     8281 ggttcagggc gaaaagatcc cttttgcttg taggcacggc ggtagctacg ccgtaggcca
     8341 tattgacgct gggggcatag gagacataac agggttcgtg ataaagaacc tcgtcccccg
     8401 gattcagaat ggcacggaga gcgagatcta tggcttcgct tacgcctacc gtcaccagca
     8461 cttcgcgcag ggggtcgtag ttgacatgga agaatccgtc cacgtatttg acgatggaac
     8521 ggcgcaggga ttccaaccca tagttggaag tgtaggaggt gtgccccttt tccagggagt
     8581 aaatggccgc ttcccgtata ttccacggcg tcacgaagtc cggctcccct acgcccaggg
     8641 agatgatatc cgtgcgtccc gtgaccaggt caaaaaattc ccggatgccg gaacggggta
     8701 tggagcttac ctgctccgct attttgttct gccaattcat gatcattcct ttcttaacgg
     8761 cagagaatta cggagcgaca gccaggcgtt ctgtctgcac gggagcttca aacacacaac
     8821 cgtttttctt gtaggttttc agatggaagt gtgtggaagt ggacaacacc ccttccagcg
     8881 tagagagttt ttccgccaca aaccgggcaa tctcccgcat gctttttcct tccaccagca
     8941 ccagcaggtc aaaaccgccg cttgccagat agcaggagga aacttcgtcg aaccgtgaaa
     9001 tgcgcatggc caggcggtca aaaccgccgt cgcgttcagg cgtcattttc acttcaatga
     9061 aggccgccac cttgctgtcg tgttcatatt cctcattcac cacggcctgg tatccgagga
     9121 tgacgccttc cttctcccac tgttcccgct gggctcgcac ggcttcttca gtcatgtcaa
     9181 gacgttcggc caattcgcca tcgctcaagc gggcttgagt cgccagtaac tgtagaagtg
     9241 atgcagagga cataaaattt caggcataaa agacgctttt acggcgaacc tgtcaagaac
     9301 catattacgc aagactgaca tcctttcttt ccaaaatgat aaaatatgtt cgttttcttc
     9361 aagaaccggc cttcttcata tagacataac aatttgtcat tcaacatcgt aaacatcatc
     9421 acaaacaacc tttggaatta ttttcagaga atagttgctc taatccgctt tttgtgccat
     9481 agtaaagtgc atagacctgt agcgtccctt gacagggaac gtttgtttcc aatttttata
     9541 tgacagtctg atattatgag catggcactt aacccggata gggctactct gaactttctc
     9601 aatgcctttc cggaagaaat ccggctgcgc ggcgagaagc tccagaaaga tggcgcagtc
     9661 acgcaaattt ttggcaatca tttatatatt cagggccgcg tggaagacac atacggcgtg
     9721 caccgcgtga atctgcgcct tcagggcaac aggtggttcg gagcatgttc cacggaagac
     9781 gaggatctgg ccggagcggc catgtatgcc gccatgctgg agcgcatgta ccgtggccag
     9841 gaccttccgg aatccccgaa cgaactgaat gatgttcctc ttctggatat tctggaagag
     9901 aaactgggac gcgaactgga cgacaaggaa gcggactacg tcagcaaact ggaaaagcgc
     9961 taccgccgtt tcgccattga acaggagatt cacgaccatg atatggtgcg cctgaatccc
    10021 cgatgggaaa tcgtcagcta tgacccgctg gaattgtggc ccgtgcctcc cacaaacatc
    10081 ctcgaattct ggaattacat tgcttacgcc ttcaacaaga aacgcctgcc ctacccggaa
    10141 ttcatggaat ccattacgga tctggaaaag gtccagcgca agattcatga atgggagcgc
    10201 tcccgtgaag tcggcacctg gtacgaccgc atccaatccg tgaatgaacg ccccccgcag
    10261 ccgccccgcg gagaactgtt catgaggctt gtttccacta ttaacgaggg acatttcgaa
    10321 taccgccatt ccctgaagca ggactggatt cccgtccggg aaaaacagga tctggaacat
    10381 ctggaaaatc tgcatgaacg ggctgccgta cgcatggacg cccagacgga aatcctgctc
    10441 gtctccctgt ccgactacgc ccgtgcggaa gacgccctga cgctggatct ggaccaggaa
    10501 gccgcatgcg ccctgatgaa caaacttttc caccagcccg ccctgaaagg ctacctggtg
    10561 aaccttgatg aaaactattt caaagtggtg gacgaaccgc tcaaatggat ttgccaggac
    10621 gatcccattg aaccggactg ctacgctctt cagcttgcaa cttccaccgg catcaacgtc
    10681 tcccactccg tccggcagct tccggggcag caggaactgt accagtctga cgaaaccgtc
    10741 tttcccggcc ccccccgctg gctggaaagc acggaagtgg aaccccgcta ctccattccc
    10801 aaacaggtca tcgacagcct tgaaggggtg gaattcctcc gtaaaatagg cgcttccctg
    10861 ccccccagca tggaagaccg cgtggtggac atcgacctgc gcggcacctt tgacatgaaa
    10921 atcgtccgcg gcctcacctc tgcggaaacg gaacacgtgc tctgcgaagt aagcgccagg
    10981 gacgcttccg gctaccgcac ggaaaaactc ggcaaggacg gttgggacgt atcccatcag
    11041 gagcccatga agaacaaagt tctgctgcgc ttcatccggg agcaccttta tcccattccc
    11101 ggcctgctgg aagaaatggg cttttcctat gatccccagg tgggcgcgtt caaggcccgc
    11161 gtcacccgcc agttccccga aaaattcgcg gaatgggcca aacagcttcc gaaagagctg
    11221 accgtcaata tggacgacaa gctcaagacg ctgctggcgg atccggtggc cgccgccgtg
    11281 cggtttgaag tagtcaacca ggaaatcgac tggtttgacc tccgcatcgt cattgatgtg
    11341 gaaggcgtcc agctctccaa ggagcaaatc cgcgccctgg tcgccgccag aggcggttac
    11401 gtccgcatgg acgacggagg atggatgcgc ctggaaatta aactggacaa tgaccagcgc
    11461 gacgccgtta cccgcctggg cctggacccc ttcgatcttt ccggagaaac ccaccgcatg
    11521 cacgccatgc agctggcgga cccgaaagcc gcagaagtgt tcgatccaaa ggcatggaaa
    11581 cgtatcaagg accgtaccgc ggaaatccag atcgacgtca agccggatgt tccctcccag
    11641 ctccaggcca ccctgcgccc gtatcaggtg gaagggttcc atttcctggc ttaccttgcc
    11701 acgaacgggt tcggcggcat tctggcggat gacatgggct tgggtaaaac gatccagtcc
    11761 atcacttacg tcctctggct gcgtgaagaa ttcgcccgca aaaacaaatc caagaaaaaa
    11821 gtcgtcatcc ctccggttct tattgtttgc cccaaatccg tgctggacgt ctgggcggga
    11881 gaaacggaaa aatttgcgcc aggcgtgcgc gtcctcgtca tccgcagcaa ggaccaggcc
    11941 aatctggaag acatcaagaa gaattacgac atggtcgtgg tcaactacgc ccagctccgc
    12001 gtctgcgggg aaaccctgaa ccaaatcaaa tggcttacgg ttattctgga cgaaggccag
    12061 cagatcaaaa acccggattc caaggcagcc aaggcggcac gggaaatcgt ttcctacaac
    12121 cgtctcgttc tctccggcac acccattgaa aaccgcctgc tggatatgtg gtccctcatg
    12181 gccttctcca tgcccggagt cctcggttcc cgctcctact tcaagaagcg ctttgacaag
    12241 cgcaaggacc cgcaaagcca gaaccgcctg gccgcgcgcc tgaaaccctt cctgctgcgc
    12301 cgcaccaaat cccaggtggc aaaagacctg cctcccagga cggaagaaga agtgtacgcc
    12361 aaaatggaag gcatccagag ccagttgtac aaggcggaac tccgccgcat tcagcaggcc
    12421 ctgctcggcc tggattcgga cgaatccgtc aaaaagaaca gtttcgccat tttgcagggc
    12481 ctgatgcgcc tgcgtcagat ctgctgccac cccggcctgg tggatcccaa atacgccaag
    12541 gaagacagtg ccaaaatgac agccctcttc tacctgctgg accagctccg ggaagaaggg
    12601 cacaaagtgc tcgtcttctc ccagttcgtt tccatgctgg aaatcatcaa gaacaggctg
    12661 gaagcggaaa acaggccgct taactatctt accggccaga ccaaggaccg ccgcggagaa
    12721 attgaaaaa
//
LOCUS       DAWEMX010000070        12472 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2142_length_12472_cov_12.297495, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000070 DAWEMX010000000
VERSION     DAWEMX010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2142_length_12472_cov_12.297495"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3701..3710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2396 a         3090 c         4022 g         2954 t
ORIGIN      
        1 ctttaagcac gcccttgcct ccgtagcgct tggcgtctcc atcccggagt tcccaggctt
       61 catgttctcc ggtggaggct ccgctgggga cggatgcgcg tccgatagct cccccggcga
      121 gggccacgtc cacttctacg gtgggattgc cccgtgagtc gatgatttca cggccgcgga
      181 cgtcaatgat ttccatttca ttcatgatcc taagatttct ttttgtcctt tttagacagg
      241 gaaacaggct ttcccgttcc ggagaagcat gtcatgtccc tagaagttcc ggtttcaaaa
      301 gcaggactga cgcagaacgg ggacgaagct tgccggcttc ttcagggcca tgatgccccg
      361 cagaatgaag aggttccctt gctgggcatc cgcctgcccg ttcttaggaa ttttgccggg
      421 aagaagctca ggagaatctg gcaggacagg atgtcggcga ggtgcgtgga ccctcaaaag
      481 ggcaggagca gccgcttttg cacggcggaa actgcggcag gggggggaag ctgcgccgtt
      541 tcgtgacgca gggaaggacg gcatgccgga acggggcctt gattccgtcc cgttccgggt
      601 atggaatgag gcgcttacta ttaaaaccat gcctaacatt aacgataact tcctcaagtt
      661 gcaggcggga tatttgttcc cggaaatagg gcgccgcgtg aatgcgtttg ccgaatccca
      721 tccggaggcg gccaaaaggc tgattcgctg cggcatcggc gatgtgacgg agccgcttcc
      781 catggcggct attgaagcca tgcaccgggc cgtggatgat ttgtccactc atgaacgttt
      841 tcacggctat gggccggagc aggggtattt ctggctgagg gaggcaatcg ccaaaaaagc
      901 ctatcaggcc cacggcgtgc atgtggaagt ggatgagatt tacgtgtctg acggagccaa
      961 atgcgacacg ggaaacattc tggatatttt cgggcccggc aacagaattg ccgtgccgga
     1021 tccggtttat cccgtgtatg tggataccaa tgtcatggcc gggaataccg ggagttccag
     1081 cccggatggt tcttatgaag ggttggtgta tctgccgtgc actccggaaa ataattttgt
     1141 gccgcaattg cctgatgagc atgtggattt gatttacctg tgcttcccga acaatcccac
     1201 cggggccgta gcttcccgga atgaactgct gaaatgggtg gaatacgccc gggcgaaccg
     1261 tgccattatc ctttatgatt ccgcttatga ggctttcata caggattctt ccattcccag
     1321 atccattttt gaaattcccg gcgcgcggga ttgcgccatt gagttccgtt ccttctccaa
     1381 gcaaggaggc tttacagggg tgcgctgcgg ttatgtggtg attcccaagg aattgcacgg
     1441 atatgattcc gaagggaaca aggtttctat cagccggctc tggagccgcc gcaccagcac
     1501 gaaattcaac ggcgcttcct atattgtcca gcggggcgcc gcggctttgt tcaccatgga
     1561 gggcatggcc cagactgccg cgctcatcag ccattacctg gggaatgctt cgcttctgct
     1621 gaatgcctgc cgtcaggcgg gcatgcgcgt gtggggcggg gaaaacgccc cttatgtatg
     1681 ggtacagtgt ccggacggtc tggacagctg gcaaatgttt gacaagatgc tgcatgaggc
     1741 gaatgtggtt attacgcccg gttccggttt tggttccagg ggggaagggt tcttccgcat
     1801 ttccgcgttt aattcacggg aaaacgtgga tgaagtctgc cgccgcatcc acagcctgtt
     1861 tgccagatag aagggcgttt tcagacagat gtccaggccg cggtgcaaaa caccgcggcc
     1921 tgtgtatttc tttccgggtg ggttgatggc cgtggcattg tatttcctgc cgggagggga
     1981 gctcctgtaa aaaggtcatc ttcctttctt acggatttgg accgggaaag agcagagtcc
     2041 gttttcaagt gatgggattc cggtaaaggg gcgtttctat ggcatattcg gcccttgccc
     2101 ccgttcattt gtacggagta ggttgttgct tatgtccccg tgggtgcgca tgagttcctt
     2161 tttccggcag gctgcttttc ctgcatgttg cgcctgcctg ctgtctgttg ctggcgccat
     2221 ccgggggcag gaaggggaag cggagcttcc ttctgtagag ccgctttcct gtgtgcatgc
     2281 cccggatggg agggtaacag ttcggggggc ggttatgggt acggtgttca cggtgcgcgc
     2341 ttatcccggg agtggcatgg acgcggaaga tgcggagagg atttgcgggg aggctctggc
     2401 ctgtgccgtc cactgggaaa aagtgatgtc cgccatggat gcagaaagcg gtctggcccg
     2461 gcttaatgct gcggaatacg gcatttccgt gcctgtttct ccggaattgg aaagggtgct
     2521 tcttctgtcc ctgaattatg cccggctgac aaacggtgct tttgatccca cgctgggacc
     2581 ctgcattcgc ctgtggaaaa agagccgccg ccttggcgtt ctcccgtccg atgaggagcg
     2641 ggaatgcgcg ctccgggcat gcggatggga aaagttgtcc gtccgtaagg gaatggcggt
     2701 caaggcggtt tctggaatga ggctggattt gggaggcatg ggcaagggct ttgccgtgga
     2761 ccgaatggcg gagatgttga aaacaagggg tatatgttcc ttcttcatag acagtaccag
     2821 cgatgttctg gccggcgctc ctcctcccgg ggaaccggga tggcggctgc gggtggatac
     2881 aggaagaggg cagggggaag tgttgctgtt gagccatgcg gccgtttcca cttccgggag
     2941 cgcccgtcag atggtgaaaa ttggcgggaa agcgtattcc cacgtgcttg atccccggtc
     3001 ggggctgggc gttacggaag ggaggcaggt gagcgtccgg gcgtcttccg ctgcgctggc
     3061 ggatgccctg gcgacggcag ggtgtgtgat gcgtgaggag gagttccgtt ccctggcggc
     3121 cggattgccg ggagtgtccg ttccggcctt ttttcaaagt ccgccatgtt ctgcggggga
     3181 aacgcagaaa caggatggac tgcggaagga gggaggaatg gggtcaatga aaggaatgac
     3241 aaagcaacta cgtttttaag atacaccttt ataaccctgt ttatgagatt gtaccatttt
     3301 ttactccctg ccgttgtcag tgctgccgta tccgcatcat ttggggcaga gtttcccaat
     3361 ccctatcctg cgcccgctcc cggtgtccgc ctgactccag agattccgct ttcaccctcc
     3421 attaatggcg cccgtatcgt cggggctacc cccggctccc gcatgctgtt ccaggttccc
     3481 gtctccgggg agcggcccat gaaaattcag gcaacagggc tgcccccagg cctgaagatg
     3541 gattcgcgcg gattgattgc gggtaccgct ccgtccggga agagggaata caaggtaaat
     3601 atccaggcct ccaacaggca tggaaaggac atgaaggagc tgattctgaa ggtgggggac
     3661 gaattgtgcc tgactccgcc catgggctgg agcagctggt nnnnnnnnnn taatgtgctg
     3721 aagacggcac ggctttttgt ggaacggggt ctggtcaatc atggctgggc ctatatcaac
     3781 attgacgact gctggcaggg caggcgcgga gggaagtatg gcgccattca acccaataag
     3841 cgttttcctg acatgaaggc catgtgcgac gctattcacg ccatgggcat gaaagtgggc
     3901 atttattcca cgccttggat gggaacgtat gccggtttta tcggagggag cgcgcccaac
     3961 gctaagtcgg actacgggga aatggccatt ccggaaaagg agcgcaagca ggaggatcaa
     4021 atctttggaa gttatccggg agttcatcgc agaaaggcgg atcatgtggg agccgtctgg
     4081 ctgtttgacc gtgacgctaa acaatgggcg gattgggggt tcgattatgt gaaagtggat
     4141 tggaatccca acgatgtgcc tacgacaaag cgcatccgca aggcgctgga cgagtccggg
     4201 agggatatcg tgctcagcct gtccaatgcc gccccgtacg aacatgtgga agagctgggc
     4261 aagctggcga atttatggcg gacgacgggg gatatccagg atcactgggg cagcgtcagc
     4321 ggcatcggtt tttcccagga acgctggcag aagcatatgc gcccgggaca ttggaatgac
     4381 ccggacatcc tccagatcgg gaagctgggc aaacccaacc agcccaacac cacgtttgtc
     4441 cagacgcggc tgactccgga tgaacagtac acccatgtga ccctgtggtg cctgctgtcc
     4501 gctccgctca tcgtctcctg tgatttggag catattgatt cgtttacgat gggactgctt
     4561 accaatgatg aggtgatagc ggtggatcag gatccggctg cccgtcccgc ccgcaaagcg
     4621 tggcaccagg ggaatttcca ggtgtggatg aaggagttgt ccgacggttc cgtggcggct
     4681 ggctttttca ataccgggaa ggagaaagga attttgaagg tgaatctgaa ggagctgggg
     4741 ctttccggag cgtatgaggc aagggacctc tggaaacgcg ctgaccaggg gaccgtacag
     4801 ggagatatgg cggtagaatt gaacgggcat ggagcatcca tgttccggtt cagcaaaaag
     4861 aagtaacggg aaggagacgg ctctatattc attcaggggc ggcggggata tgccgcccct
     4921 gaagcctgtg gcaggggcgg cttttcattg aaccggcatc catagaaaca agccttctgc
     4981 acaaaatccc acttttacag tacctgtcgg cggattgtcg gatttcagga ggggcaggag
     5041 gggcagtgaa tgcctccccc gcaccagagc ttgcgcttca attccggaaa gtttttcccg
     5101 gcaggccttt gttattgtgt aataaaaaac cgctccggca aatccggagc ggtttttata
     5161 aaagagattg atgtgacttg ccttaatctc tcccgacagc ttttctaggc ggcaatgccg
     5221 aaatgctgct gaatggaggc gaaaatcttg tcgcgacggg cgcgggcttt ttcaatgtca
     5281 cgggttttca gcgagaatct caggcgttcc gccgtggagt ccggcttgtg aaccgtaacg
     5341 tggcaccacc aaacccccct gttgttccac tcgtggtggt tggggttgtc atcattaatt
     5401 ctaagagcaa ttttggtttg ggctttcatc tgtgtgatct tttccagtag aggacgtatt
     5461 gtttacgtcc gttggcatat taatgtttca ataaaacctt caacttggag gtgcctcact
     5521 taagcagatg atcccttgtc tttttggaca tctttttctg ccagttgctc acctcgttga
     5581 agccttagag ttcaagtggt ggaacaacgt tcaaaaaaag aatctttttt acaggagacc
     5641 tactttttta agagtgttgt aagcaccgtt cttgttgatc gtacgcaggg ccttggcgga
     5701 aatcttcatt ttcacgaatt gaccgagttc aggaacccag atgcgcttct cctgaaggtt
     5761 ggggaaaacg gtacgattga cagtcttagt gacgtgacga ccaataccgc cctttttctt
     5821 ggcaagaccg gagcgatgga tacgacgacc tttgtgaggc atggtacctc tgatgatgca
     5881 aattcgggac atggcaatcg aagttatttg ttaatgcgag agagataatc tagcgtattt
     5941 ttgcctaagg tcaagaacaa ccggacgaaa gcactggaat ttatcctgga ctgtacggaa
     6001 gcatgccttt tcttttgtac gaattccggt tttttctccc atgatgaaga ggggctgcag
     6061 agacggtgag ctgaaagcgg gagggggctg gtccgggaag atgaacggta ccgcagacaa
     6121 agaggagaat gaggcgttct tcaatatttt tcaggacttc cgccgtggga gcaggggctg
     6181 gccggatagc cgggccggat gaaggccttt caccgcagga aaagtgcaga acacgggaaa
     6241 gatggcccct ttggtttttt cacggcagcg gcatatggct gcctgcttcc gggtaaaacg
     6301 tatccggcgc ttgcacggag gggcgttcca tgagaaattg aaagcatgag tgagaatcat
     6361 tatgcagagt tttccgaatg cagtatgaag ccccaggagg tgctggaata tgtttccggc
     6421 cccattcccg ttccggagga gggagaagtt ctggtccgga tgaaggcggc tccgatcaac
     6481 ccggcggaca tcaattttgt acagggagtt tatggtctga agcccgtgct gccgcactcc
     6541 cgcgccggtc tggaaggctg cggcgtggta caggagtctc gcgcagaggg attccgagag
     6601 ggagatgtgg tgattctcct gcgcggcgtg ggttcctgga gcgagtatgt ggcggttccc
     6661 tccgtgaatg tcatgaagct cccggtgaag gtagatcccg tccaggcggc catgttgaag
     6721 gtgaatcccc tgaccgctct gcgcatgctg gaagggttcg tttccctgga accgggggat
     6781 tggctggtgc agaatgccgc caattccgga gtgggaaggt gcattattca actggcccgt
     6841 gaaatgggcg tgaagacagt gaattttgtg agaaggccgg atgaattgag ggatgaattg
     6901 actgcgctgg gcgccgatct ggtggtggga gaggatgacg gggatgtggt gaagaatacg
     6961 ctggccctcc tggatggaaa gcggcctgtg ctggcttcca atgccgtggg cggggaaagc
     7021 gccctgcgcc tgatggatat gctggctccc ggcggaagca tggtgacgta cggagccatg
     7081 agccggaaga gcatcaaggt gccgaacggt tttctgattt tcaagggtat taaactggag
     7141 ggcctgtggg tgacgcagtg gcttaagaat gcccctgttt cagagattga ggccgcctat
     7201 gagaaactgg cgcgcctgat ggcggacggc aggttgaagc aggctgtgga taccgtttat
     7261 ccgctaagcg atgtgcggaa ggctgtggag aaggcgcagg aggagttccg cagcggcaag
     7321 gtggtgctta gcatggattg cgcctgatgc gtggaaaggg agtttttgcg ctttcccgta
     7381 ctgcggaatg ctggaaggat tttttcagcg ttccgtggtt tgggtagtat tggctgctgg
     7441 cctggatgat ggcggggctg aacttttatt ccatctggca ggcaggcgat ttgttctgga
     7501 aaagagccgt tttgcccgga aaggtctgtg tacggaggga atggtgcggt tgtggcgcca
     7561 ttcgcggatc atgaaaccgt ccggactttc tctggatgtc tgggcgccgg aagtgcgttc
     7621 ccatccgcag acccgggagg gacggcgcgt gcgcgccgtt tggtttcagc ccagcgtgga
     7681 atcaagccag gtgttgagta tttccacagc ggctacctgg tcgatgatgg ggcggaagtt
     7741 ttttgccttt ttgccggcgg cgtgcagttt ttcctgggca atgacggtgg tgaggcattc
     7801 atccatgaaa atcaggggaa ggccgggaag ggctgcggcc agttcccggc cgaatgcgcg
     7861 caccttggct gcggcggtcc cttccgtgcc gtccatgcgg acgggaaggc ccagcaccag
     7921 ggtgcgtatt ccccgttcct ggacgagctg gacaatgcgg gaaattccgt ccgtctgatg
     7981 cctgtggata gtttctacgg gatgggccat gatgcccacg gggtccgtgg cggcaatgcc
     8041 gatgcgggct tctccgtagt cgatgcccag ggcggggtgc ggactggtca tctgagttcg
     8101 tccggaagct ggttgacgat tttcttgatt tcgtccgcct tgtttttatc cgcaatgagg
     8161 atggcgtccg ccgtttccac cacaatcagg ttttccactc ccagcagggc cacgtgcttg
     8221 tccccctttt gggagaaaac aatgttgccg gaggcgtcct gcacggtgat gtccgtgttg
     8281 gtggtgtttt tattgtgggt ttccagatag ttggctacgg atatccagga acctacgtcg
     8341 tcccagtcaa aggttgcttc catgttcagc actctgtccg catgctccat gagcgcgaag
     8401 tctatggaga cgggggtgag cgccgggaat tcttcccgga tggtttcctg cggggccgga
     8461 gattccgcaa cgtgttccac gaaggaggcc agttccgggc agtgcttgtc cagctgttcg
     8521 cacacggcag gaatgctcca tacgaacatg ccggcgttcc agcagaaatt gccccggctc
     8581 aggtaggctg cagccgtggc cgtgtccggt ttttcgcgga attgaatgac ttcgcggcag
     8641 gaacagcccg gagcggagtg gatttctttt ccgcgttcaa tgtagccata ggaggggcag
     8701 ggccaggttg gcttgatacc cacggtgaca agcgcttttt ccctggcagc cgtgtccatg
     8761 gcggctttca tcagggtgcg gaaggagtcc tggtcctgaa tgagatggtc ggaggggata
     8821 acgagcatga ctccgtgcgg gtccgccgcc ttgatgagac caatgcccag ggcgatggca
     8881 ggcgccgtgt cccggcgcac gggttcggga atgatgttgt ccacgggaat atcgtgggcc
     8941 tggcggcgca cttccgcttc ctgcagatgg ttggtcagga taaagatgtt ttgcctgggc
     9001 accagcccgt ccagcctgtc gatagcctgg cgaaggaggg ttccttctcc gaacatgttc
     9061 aggagttgtt tggggcggtg gtcgcgggaa agaggccaga aacgggtgcc gctgccgccg
     9121 gccagaataa gagcgtattg gttcatggct gtttcgcagg aatgggggtt caagagggtc
     9181 agagagcgcc gaaacggcgt tttctacggg agtagtccag cagagcagct tccatgtccc
     9241 gatcgcaaaa gtccggccac aacgtgtccg tcacatgaat ttccgcgtag ctgatttgcc
     9301 agagaagaaa gttggagacg cgcatttctc cggaggtgcg gataagcagg tccggttccg
     9361 gcatgccggc ggtgtccagg tactcgccga acaggtcttc cgtcacggcg tccggcggga
     9421 gttctccgcg ggagactttt tccgctattt tcctggcggc ggcggcgatt tccccccggc
     9481 tgccgtagga aatggccaga accaggttga gcctggtgtt tcccgaagtg gcttccagac
     9541 tgttctgaag cagtttccgg gtgcgggcgg gaatgcggga gagatctcct atggcgtgga
     9601 ggcgcacgcc tttttccatc atttggttga ggcgtttttt caggaattcg tgcagaagga
     9661 gcatcagcgc gcgcacttcc gtcttcggac ggttccagtt ttccgaggaa aaggcgtaaa
     9721 gggtgagcca ggggatatca tgggccatgc agaagtccgc acagcgttcc accgtatccg
     9781 ctcccgcccg gtggcctgcc tgccgcggca gatggcggct gttcgcccag cggccgttgc
     9841 cgtccatgat gcaggcgatg tgagtcggca gtttttcctt gggaatggta agcatagata
     9901 agcgtcgctc cgttactcta gtccatgacc gggtccagtt caacacgcaa gttgttcagg
     9961 atgtattcct gtctttcccg cgtgtttttg cccatgtaga aggacagcag gtcttttacc
    10021 ttgtgggaat cttccagccg gacgcggtcc aggcgcatcc cttccccgat gaaagccttg
    10081 aattcctggg gggagatttc tcccaggcct ttgaaccggg taatttccgg gtttttcccc
    10141 agcaggctga tggcttcctc cctctctgcg tccgagtagc agtaaatggt ttgctgtttg
    10201 ttgcgcacgc ggaagagggg ggtctggagg atgaagaggt gtccctcccg gatcaattcc
    10261 ggaaagaact ggatgaagaa tgtcatcaaa aggatgcgga tgtgcatgcc gtcgtcatcg
    10321 gcgtccgtgg cgatgactac cttgtcatag cgcagttcct ccagtccgtt ttcaatattc
    10381 agggcatgct ggaggaagtt gaattcctca ttttcataga cgactttacg gctcatgccg
    10441 aaggagttca gcggcttgcc ccgcagggag aaaacggcct gggtttccgc gtcgcgcgcc
    10501 gtagtgatgg agccggaggc ggagatgcct tccgtaatga agagggttgt ttccccggcg
    10561 cgggagtgct tggagttgta atgcacccgg cagtcccgga ggttcttgtt gtgaattttg
    10621 gccttgcggg cgttttcgcg tgccagcttc tgaatgccgg agagttcctt gcggtcccgt
    10681 tcggaatcct tgatttttgc ttccagggct ttggccgttt ccgggttttt gtgcaggtag
    10741 ttgtccagct ccctggcaat gaagttgccc acaaaggtgc ggatggtttc cccttccggg
    10801 ctgatggtgt tggaccccag cttggttttg gtctgggatt cgaagacggg ttcctttacc
    10861 ttgatggaga tggcagctac gagggaggaa cggatgtccc ccgcctcata gtttttcttg
    10921 aagaagtccc tcagggtttt gacgatggct tcccggaagg ccgcctggtg ggtgcctccc
    10981 tgggtggtgt gctgcccgtt gacgaaggag tagtgctcct ccccgtattg gccgccgtgg
    11041 gtgaaggcca cttcaatgtc atgcccttcc agatggatga tggggtacag gggttcctcg
    11101 ctcatcgctt ccgcaagcag gtccagcaac ccgtttttgg agatgtagcg gcggccgttg
    11161 aaatgcaagg ccagcccttt gttgaggtag gagtagtatc tgcacatttt ttcgatatac
    11221 tccggccgga attgcgtggc cgcgggaaag atgtccgggt ccgcgtggaa agccgtgcgg
    11281 gtgccgttgg gttcctccgt gggtccgtcc ttccgtccct tggggatttc ctttccgcgg
    11341 cagaactggt aggaacgcgt ttccccgtcg cggtaggcct ggatttcaaa gaagtcggac
    11401 agggcgttga ccgccttgat gcccactcca ttgaggccta cggatttttt gaaggcgtcg
    11461 ctgtcatatt tggccccggt gttgattttt gcggcgcagt ccagcagttt gcccagcggg
    11521 atgccgcggc cgaagtcgcg cacttcgcac aggccgtccg gagtcacgtc aatggtgatt
    11581 tttttgccga accccatcac gaattcgtcg attgagttgt cgatgacttc tttcagaagg
    11641 acgtaaatgc cgtcgtccgg ggaggctccg tcacccagtt tgccgatgta catccccggg
    11701 cgcatgcgga tgtgttccct ccaatccagg gttttgatgc tgttttcgtc gtaggccatg
    11761 tgcaacgggg tcgcgcttat tatagcggcg cccccggaat ttgcgaagaa aaaatgaggg
    11821 gccgtccgtc tgcgttcgat ggagcttgac ccggaggccg tcccttggtg gaatccacgg
    11881 cgtatgatca gttgcaaggc tccgtgcaaa gtgaacgttt ccctccgcgt tctgggaaag
    11941 aggccggacg gcttccatga ggtggatacc gtgatggttc ccctggattt gtgtgatgtg
    12001 ctggagtttt cgccggcggc ctgtctggag atgagctgcg acgccccggg cgtgcctctg
    12061 gatgagagca acctggtcat gaaagcgggc cggctgatgg agcgggagct gggaaggccc
    12121 atgccgtggc atgtccgcct ggtgaaaaag gtgccccatg gtgcggggct gggcggcggc
    12181 agcagtgacg ccgcatgcgt gctgcgcacc ctcaatgagc tggaacgcgg aggtttgtcc
    12241 cgggagcgcc tggcggagct gggcggggaa atcggttccg acgtggggtt tttcatttat
    12301 ggcgcggcga gccgctgcac cgggcgcgga gagaaggtgg agcccttgcc ggagtggaag
    12361 gggtggcgtc cccgggttgt cttgttgaag ccgtcgtttg gtgtctccac gccggacgct
    12421 taccgccgct ggtccggttc cagggaattg cccggcattc cctatgggga gc
//
LOCUS       DAWEMX010000071        12379 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2162_length_12379_cov_13.175917, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000071 DAWEMX010000000
VERSION     DAWEMX010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12379)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_12379_cov_13.175917"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    254..263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2454 a         3668 c         3546 g         2701 t
ORIGIN      
        1 ttatccagac tactcttgct ggaatacaaa gaatttatag atggtattgc tgttaaacag
       61 ataagagtga taagaatcag caaaggaagt acctctgagc aatggttcgc atactgtgca
      121 tctacaatga tagcataaat acaatacaat gttgtcaata aaaaacaagc ggtagggatg
      181 acaaataatc ctgtaaaaac tatttttctc cacagaggct cgtaggatac tctttgtact
      241 gataggattc catnnnnnnn nnnaggcact ccatgaaata tttgcctgaa gcgaaatatc
      301 gccattacat aaattaagac acaaatctcc agatcctgaa gcattaacac caaaacgttg
      361 tcgattaaac tgccccgttg atttatttct ataaccgcga cgtctgtatt ctataacaga
      421 aaaggcttct gcagatccac atactttaat attagataat tttttacatc cactataaga
      481 aattttagct ttagttccta ctctaattcc agcatcaaaa tgtatgccca aatatttgga
      541 agcaagttta tcaattttct taacgcctgg tattgtccac tgccaagcta cataaaattc
      601 gcccgaaact ttgaagtagg gattagtttg tatttctcct tgtacttttg cataaaatgg
      661 agccccaatg acccctagat ttacttcata ggaaacggtg tataacccaa taatatcatt
      721 agatattgtg ggcgaattat agatataaga gtataaattc caaccacctt cttccttgat
      781 gggatcgcgg ctgatccacc tgccgtcaag cggattgaga tgccggtagt tgtagtagac
      841 cagcccaagt tcgtcgtcca tgtattcgct cgagaagcgg aatttgttct cttccgccat
      901 gttgccttcc gacgtaatca gaccgccata cggccggtat tcatacagcg cgcggcgtcc
      961 gcgcgcttcc ccgaagaggg aggtgacgtt tttcatcgcg tcgtgcatgc agtagagatg
     1021 ctctttctcc tgcgtcccgt tcgcttccca gcgcgtcatg gacaggacac gcgtggcctc
     1081 aggctccgag gggtcccaga tgtaactgcg tacaacctcc ggcgtttccc cggtcaagtc
     1141 gcactccgcc acctgcaggt agtcacggta gaggtagcgc gcgtggaagc ccgttgtccc
     1201 tccaaccgtc acttttttct cgaatctcct gcccatggag tcgtaggcgc attccacggt
     1261 tgtccctccg tcttcgcttt cgaatttcac gggccggttt tccgcgttgt aggtgacttc
     1321 ccagatgccc gtggatgttt ttaccagcgt ctggttgccg tccgcgtcgt attggggtgt
     1381 aaaggcttcc cctccatttc tgacgatgcc cgcataccgg ttgagttcat cggtttcata
     1441 gtccgtggtg ctgccgaatt cgaaagcttc cttccggttg ccgatgttgt cgtactgata
     1501 gccgaagcgg ccgccgggcc tgagctggtc tccgaccagt tcgccacggc tgttgtaggt
     1561 gaacaaacgc gtcgttttgg gcgccgccgt gttccacgtg tccctgcgcc gggtaggacg
     1621 gcccagcgcg tcgtagtcgt attcgtgcct tgccggcggg ttggcgcttc cgggcctctg
     1681 gtagtcgatg acggagagga ggtcgcggct gtcttcatag gtgttgaccc gggtcatgcc
     1741 gttggggtag gcgagttgtt ccacaaaccc tccctgttcg gagtaggtcc agaagaaggg
     1801 ttcttccatg ccttccgccg tgagggtgga gaggcggcct ttcccgtcat aggacaggtg
     1861 cgtctgctgg atgcggcggc cgtccgcgct taattcgtac cccgcctgac ggccaagccc
     1921 gtcatagcgc tcggagacct gccaggcgat gccgtttgcc gcaaggctgt cggtttccag
     1981 ttctccgtag ggattgtagg cgaaggtccg cgttccggcg gcatcagtga tttgcgtcag
     2041 attccccaaa tggtcgtagg cgaagctctg gccgggcgtg gcgtccgagt agctgattcc
     2101 caccagcagc ccgcgggcct gttcgtaagt gcaggtcttc acgatgccgc gggcgtttgt
     2161 ttccgtgaca agcctgttga aggcgtccca ggttttgtcc acatgcgttc cgtcggcata
     2221 cgttttgcgc gtttccagcc ccgtggcttc gtcatagctc caggtggtgg tgtcgccgtc
     2281 cgcgcgctcg gaggggtcct ccgcgatatc tccttcctgc gccgcgcgga aggtggtcag
     2341 gctcaccaga cggtcggcct cgtcatagcc caggagcagg ggctgcgtcc ccgtccccca
     2401 ttcggccgtt ttccgacctc tggcgtcgta tctggcgcac accgtatttc ccagcgcgtc
     2461 cgtcaccacg gcggccaggt cgtggcagac gtcgtattgc gtcacggttt cattcccggc
     2521 ggcgtccgtc acgctgacgg cccgaccggc caggtcagtc cggatcgccg ttgtgttgcc
     2581 gcggccgtcc gtgcgggtca gggtcatacc cgaggccgta taggcgcgag cggccgtctc
     2641 cgtgatgccc gcgtggtttt tctgcgagag cataaagccg tccgccgcca ccgtttgagc
     2701 cgtgacgctg gaagagggga taacgctctt ttggattctt ttcgtatttt cagcatactc
     2761 cgtccattcc gccgaagtca agccgcgttc gtctatgatg accgcttttg tttccagaac
     2821 tccggagagt tcggacagga gctgcttccg tacggagaca agcggctgcc cctcagcatt
     2881 gtaccgggtt tgcgccgtca ccatatagat gccgtcctcc gcgttttcca cgctgaaggc
     2941 atattcccga atggggctgt tgtccggaac gggctgctcc gccagggcga gcgtttccct
     3001 ggtcatgttg ccgaaggcat cgtattcata gagcgcaggc gccatggcga cggccccctc
     3061 ctgcgctccc gcatccctcc atgagcggat gagacgcccc tgttcatcgt attcggaacg
     3121 gtcatacagg aacccggcag tcgtgggcgt cacttgcacg acgttttgtc cgaagccgtt
     3181 gaccagggtc cgggaaagga tgatggcctg gccggccagg gtaacggttt ccctcaaaca
     3241 gttattgtcg atgtcataga catggcagcg ttcgcgctgt cccgtgccgt attcatgaag
     3301 tacggagcca tcggcatgat attccgtgac gagggtagcg ccggagggcg tcgtgacggt
     3361 ttccgtaagg ccgccttcgc tgtaggctgt cgccgtcacc cggcccagca catcggtttt
     3421 cctgacgata cggccgagcc tgtcgtattc cacgctttcc gtcgtggtca tggctcccct
     3481 gtcccggcgc gtctgcaggg cgcgcccggc ggcgtcccgg gtgtaggtgg tgatggtttc
     3541 cggggtaacg accgtgtctc cgtcgctgat ttccgaacgg atggtttcca ccagctggtg
     3601 ggcgctgttg taaccgtacg acgtcaggac accatcttcg tccgtttccg agagcttgcc
     3661 gcagcacatc caggacgtgg cgctgacgcg gccgttgccc cgcgtggttt tcgtgcggcg
     3721 gccttcttca tcgtattcat gggcgccgct tgagagcagc agccagtttt caccatccca
     3781 gatgctttcc tgctcaaaaa gcaccgtatc attggcggca atgaacgatt ccgtcttgcg
     3841 gctctgtccg gccaccagtc cgccgccggc tttggtgatg accgtctttt tatgcgcggc
     3901 gccgtgttcc gcggccgcct cataatcata ggaggtttcc actccggcaa tgtcccgggt
     3961 gaatttcatt tgcccggcgg catagggata ggcggcggct tctccatacg tttcttccac
     4021 gcttgtctgt tcctggctgg aaccggcggc agtcacggtt ttgacaaccc gttccatgag
     4081 gggagactgc tcataggcgt aagcgacggc cgtcagttcg atttccgtgc cgtcggacag
     4141 caaccgggat tcggcgacac gcgccggacg gttgtcgtag aagcgcaggc cggcgtattc
     4201 ggtccgggtt acctgttctc cgccgccggc ccagggctcc gcctcaagcg tgacccgtcc
     4261 ttcgccgtcg tattcatagc gcgtgtagcc tccgtccggg cggttgatcc gggagacgcg
     4321 gtactggctg ttgtattcgt aggaggtcgt ccgcgccagc ggcgtgttga aggcttccgt
     4381 ttcgcttacc agcagccagc cgccgtccgt gtattgcctg acgctgcggc tgcaggaaac
     4441 aggctcggca tcgttgatgc ccctgacggt ctcaatgcgt tccgagaggc ctccgtagag
     4501 gcggttggtt tcaatggtgc ggatgatggt gtcgtccccc tggcctttgg cgatgctgac
     4561 tctgccgggt tcttccgtac gggtgatggt gtgggccggc agtccgcgct gctggcgcgt
     4621 gatgacggtg gtccgcacgc cgttttcttc cgaggacagg aaggaggagg ttttataagg
     4681 ttcccccgtt acctcgtatg ttccgtcggc caggaccgtg acggcggcgg gagcgtacca
     4741 ttccatgacg agctctccgt ccgctccggt ccgggtgcgc atcagcccct ccgcggcgct
     4801 gtagctgctt accaggctgc cctcatggtc atacgtattg ctcacattcc ggaagtagtc
     4861 ttccgccaga agaaccctgc cggaggcgga ggtcatgccg accacagcgc cggttttcgc
     4921 ggagaagcgg attttctgcc cgtccgcgtc caccagggaa aggtaggcgg gggcgccgga
     4981 agcggcagga gccagagttt catccagcag ctgcaccctg aagtcgcgct tgcgggagat
     5041 gcccgcgggg agggccgtat cggaaccggc ctgcacgttg aagtaaagcg cggcgcccgc
     5101 cggaggcacc acggtgatga ggccggacgt ttcgtccaga tgggcgcgcc aggcccacgg
     5161 gctcacgtat ttcaggccgc cgcaggcttc cgccgtttca cgggcgtttt ccgcggagcg
     5221 gaggctgaag gccgcgggag acggagccga agcccctccc ctggccagga attcggcctg
     5281 ggacatgcag atttccacgc cggggtcacc cggcagtttt tccgccgcaa tggtgtattc
     5341 attgccttcc gcgtccgtgc acgtaccgca acggcagcag atttcctttt cctccggcgg
     5401 cacgtccgaa gaggagctgc tcgacaggga ggaaggaggg gtgtccgaac tgcttgaact
     5461 gctgctggag tccccatgcg cggtcaccgt cacggagtac ctgcacacgg actggttgta
     5521 ttcgttgtgc ggcatgtcga tgttctggtg cgtgatggag agagtgtacg tgccttcgga
     5581 taactcaaag gagcctgatt tggaccattc ctggtggcct cccagttttc cgggttcatc
     5641 ggcggcggag gtcaggtcca gctcaaacgt gccgcccggg ccggaaatgg tcagcttgcc
     5701 ccagtcatcc accgtcaggc tggccacagc cgtggccccg ccttccggga cggtaaaggt
     5761 gtcgttgact ggagacatga aaacatcggc tccggcagac gtctgaacga agttgtcgga
     5821 tttctcataa ataacggaat ccgcgccgta tggctgaatg ggtgtggaca tggcattgag
     5881 gggcgctgcg gcgctcggcg cgccgggctg gctggccgca tcgggaagcc ccgcggctgg
     5941 gttggagtta atcatgtttg cggaacccct gttcaaagag ggggcgtcat tttgttgatc
     6001 gttggtaaac attgtgtgat gattgttgtt ttattgcggc gcgaaaggcg ccgattggat
     6061 tggttcatct tccttccccg tgccgcacca cggcggacgg atgggaagaa cagggaatgg
     6121 aggccatgcc cgtcaggaca cggactccgg aaaaggaagc tgccggctgc cggcggcagg
     6181 ggtcagggaa aacggaccgc gggaaaatcc gctgccgttt ttttccagag cttccggcgc
     6241 ccagtcatcc cagaacagcg tgagaagccc gtcctgcagg acgtagcgcc cggcgcagcc
     6301 gccggcgccg cgcatgaaac ggccccgggc aaaaaacagg agttcgtccc tccaatgggg
     6361 atgcacggcg tcataaacgg ccaccggctc atcatggaaa agggcctcaa tgccgtgcat
     6421 cctgcccgga gagcaccaat gggcgctcac ctggtcattc aggcggtggg gcgccggagc
     6481 atggctgagg aagaggcgcg gcgtggcatg gacgcgcgcg cccagttccc gcgccagccg
     6541 gccgaagatg acgtcctccg ccccgacggc gggaacccgg ccgccgtgcg ccacaatgcc
     6601 ctccacaagc gcccggctca tcaggtagcc cgctccgccg ctggggaacc ccctgtccgc
     6661 cagggacatg tcgcccacaa ggtcatagcg gccgtcgcag aggctttcca ggcggtcaag
     6721 cgccagccag gtatcgtcgt cgcacttgaa aagccagtca aagtcataat gttccagggc
     6781 atattgataa aaggcaagcc ccttggcggg caggtgcctg taatcgtcct ccacccaaag
     6841 ggccactaca tcgggctcat tgggcaaagg agtgcgccgc cccaggaaaa atctgcattc
     6901 cacaccctcc tgaggatggg aaagccaggt ctcccggcac gccttgcggc ggttcgccgc
     6961 gcctgtgcag ctgcagatgc ccaccaggat attcactttt ttacgcggcc cgggggcagg
     7021 cttccaggcg tggcccggca gatgcctgtc cagcagctcg cctgccatgc tgctgcggct
     7081 catattttcc caggcgtccg gctcccgcct ggccatctct ccccagaggt ggacgcacca
     7141 ggcattggac aattccggcc cggcaaggcg tatgctgccg ttgtagcagt cgcgccatct
     7201 cgtccagggg accggataca tgtgggaaga cggagcggcc cggtcaaaca ggccgcagtg
     7261 gcgcaacgcg cgcgtcatcc cggtggggcc gcaaaatccc catggcacct ggcgccggcg
     7321 atcggcgaca tccggcacac gtcggagcag ttcctccttg gcccggactt cttccggaga
     7381 gtcccagggg acgcggagcg ccggatcctc agcaaggcgg cacagggttg cgggaacgga
     7441 atgaccggga ggaaaggcca tggcgcctac ggccaccacc tccgcccact ccctgcagaa
     7501 ccagagagga gaggcaggaa gttcatcacc caggcagatg acgtccatat ccacccagaa
     7561 gccgccttcc tgtgaaagga atttcatgcg gaaccaatcg gaaaacggcg ccaggctatt
     7621 gtgggaatca tggaaaatcg cctcctccgg gagaatatcc cgcgcatcgc gcacaagcgt
     7681 tcccgcggga atattgtcgt aattccggta ggtgaaaagc tggaaagcat ggccatggtc
     7741 caggaacgat ttgatgcaca gaacggacat caggggaagt tccccgccca cccagaggct
     7801 tgtaacggtt ctcgtattca ttgttttacg ggtatgggtt cgtggcggaa ttacagtgtt
     7861 acccaatggg gcaggtaaat atcccggtag tcttccacac cgccgacaaa ccagtcgcgc
     7921 ggaacgatga ccttccgggt atttcccagc cacgcgcccc accaggaaaa ggaagaacac
     7981 gacatgacga gttcctccat cgccgtcatg cggcggaggg attcgcacgg agcgccgcgg
     8041 tcgatttcca gcgcaaagcg tccgaatgcc ggcacgcgcg caagcatctc cgccgcctcg
     8101 tccggctcat cgctgaacag caccagacgg ttcttcccgg atgataaatg ccctgcggcc
     8161 ctccgcagga aaccagggtc cagaatgcgg tgcttgtcac ggaggcgcct gtagtcccca
     8221 agccggatgt gaatgccaac ggcgccgggc tccttctccg cggtgagagg cgcgaaaagc
     8281 gcccggatga acgcttcctg cccttcaaaa taccgcgcgc tctggaagta gccgcgaagc
     8341 ccgcccgtcc ggatgcggga gggaaccgga caataggcaa aggaaggttc ctgccaggat
     8401 ggaggttcat tggtgccgca cggcgtagaa gggagcaccc agccgccgag ccgggagcgc
     8461 agcatcaggg aagcgtcatt gtaagcccag ggcacccgac actccacccc tacggtacgg
     8521 gcgtgggcac aggcggccgc cagcgtgaac atgatgttgc ccagccgcat gccgggcagg
     8581 aaacacgggg aaacatattt tttattcata aggccggttt tccgttccat gttcattccg
     8641 ggagtcttgc catcgcctcc agcacttcct tccagctctc cagggaaaca tccaacccgc
     8701 ccagctccag gccgcgcgca cgagccgcct ccgccatgtc gtcgcgaaga ttcggttcac
     8761 aggccatttt ggaagcgtag tagatgaaat ccctttcgtt gctgcacagc catcctgttt
     8821 tgccgtgctc caccatctgc cgccacccgc cccggttgtc cacaatgagc acgctgccgc
     8881 tggccatcgc ctcaaagccg atgcggggcc agttttccgt cgtatccgtc ggctgaagga
     8941 tgatccggct gtgcctgtaa aaatcctgct gggaaacttc cctttgattg cgggcaatcc
     9001 gaatccagtc gaagggccgg cctatcttgg cttcgctcct cttgtcaaaa ccgaggaaaa
     9061 ggccgcgctt ctgcacgggg gatacgaatg cgccgtagat atgcagggtg ttggccgcga
     9121 acttgtcggc atcctggcgg gagatgcgcc cgcagccgaa gaaatcctca tcgcgttccc
     9181 ggataaaggg aaaggattcc gcatggaaat agggcctgaa ggtcaggaac tgcacttgcg
     9241 ggtcgccgtt gagcttgcgc agcacgggca tggcttcctg gcggacggct tcattctgat
     9301 agaggaacat ggcgatttcc cccttttgca tggcctcctt ttccctgggg aaaagccacg
     9361 tcatgcagtt gatgaaaact gtgcgtttcg tatgcctgcg tatttccggc aaggcgttga
     9421 ggaagccggc attgcagaaa ccgagcacgg ggtcgccggg ttggacggcc gaccagtccg
     9481 cgggggcgtg catgatgacg cccagggaaa ccatttcgtt gtaaagaggc tccccgtgat
     9541 aatcccagga cgggatgaga tggacttcca cgcccatctt gcgccagaca atgatctggt
     9601 ggtgaagttc cgttcccgct cctccgtaga ggctcgggaa cccgttgacg aataatctcc
     9661 tgattggact gctcatgagc gctgctcttc gttgcggagg gtaagaaatt taaccaatgc
     9721 tcctgataaa ggaggagaac tccggtgtac tgcggcccgc actgcgcagc gcgggaatga
     9781 aaatgccatt atgttccagc ctcctgagcc tggtcgtaag cttccacttg ccggcgaata
     9841 cttcattcct cagggcaaat ctggaagccg tgaggatgtc gcgccccgca aggtaataag
     9901 ccatgccgcg cagggaccag gcagccagcc cgaaagcctc tccttcgtac tccaggggaa
     9961 agggataatc cacgccctcc ggagccttga aaagcgcctg cacatgcaga acgggagcga
    10021 aagaaggccg gtttcccggc tctctgccga ccgtgccgcc ggcggctctt acttcttcca
    10081 gagaattgaa gacggccggc ttctccacgg aatcaaaggg gagtttttct ataaactgct
    10141 tgcgtgcgga aagcacggaa agctccagag gaacgcttcc gtcggaaaga acaacctccc
    10201 tgttgaggat gatactgccg gggggaaata tcccggaaag ggcgcctacg ctttgaacaa
    10261 tgttcaggcg gggaaggaca atgtcgttgt tcctgatatc tccggtaacc gtgcctacgg
    10321 gaagcagggg ttcatgagag gggcctgccg cggcatccgc cacgggacag gaaagggaat
    10381 tttccgaacg ctgaggccgg gaactggaat tcccgcatgt ttttgttgta ttcatcattt
    10441 tgtttttcaa atgcttatgg ttcttgataa agtgaataaa cggctcttca cgaagctgtc
    10501 ccggacaggg catgcgccag aaaagaaaga acgttcatct gccttcacta tgattgcttg
    10561 ccgcgtttcc ggaaattcgc cgcccatttc ttaaaaaagt gaatttttcc tcccggaggc
    10621 gcttcctgat tcggcgcgct ctgctttcct ccagacgaat gcttctggca gcccgtgaca
    10681 aattcccttc agcgtcaatc aatgcctcaa ccagcgccct ttccagcggc tccagagatt
    10741 ccatttcccg cgcgaattct tcccggaaca tcagcccgga aaggaattcc gaatgctcat
    10801 cagcatcaat ttcccggcca ttctcatatt caagaggacg ggcttcccgc agcagcagcc
    10861 tcttcttccg gttcctctcg cgcaaatagc gttccggctc aaaaacggtt gcattgccca
    10921 ggtatgtttt catgccggcg gcgccgcctt tccggtaacg gcggcaaatt tcccaacatc
    10981 ggattctgaa ccgttgaagg acatccagat actcttcctc aggtatctga aatccgatgg
    11041 attttgcttt tgaacggagg cgctgagcga gatataaggc gtggttttcc acggtttcca
    11101 acaaggatgg tgattgcttt tcgtgcatat tcaccaaccc tgtgcggact tatccagcta
    11161 gcgaaaaaca ccccactttc acatcatcat tattatttga aattcatata tttaccaaaa
    11221 gaaagcggct agctggacga ataagctcat ccagctagcc gctcatgggg agggtctata
    11281 accgcctgcc ctcctacctg tgcctttccg gcttcctttc aagcgaaagc atcagttcca
    11341 aaggacggat ataagattta cagggcacta cctggaaccg gcggtacatg ccctgccttg
    11401 acttctccct tttcttggca aacgggtctc tttccgctag ctggggcagc ctttccacaa
    11461 gaacttttct caaatccgac ctggcttccg cctcattccc tatcccggcc cttttcagaa
    11521 gctccttgtc caaacaggct ttatccggat tcattacgta caaaaacagc atcttataag
    11581 ccggcttcgt ggaatcctga cccgccgccg cccgttgcct gctgaaaaaa cgtttttcct
    11641 cccgatagga aaacctcatg aattccttca cttctccacc gccccgtgaa caggaaaaga
    11701 ctatttcccg ctcatcggtc acggaaatcc acagatcctc ccacatggtg ccggttggaa
    11761 ggctttcccc cgtgcgcgca tgcacaggca tgccctgcct gagccactcc caggaatagc
    11821 cgtatgccca ggcaaatcca tctttcgtcg tcaccatgac gtcgggaatg gaaacaacga
    11881 tgcctttgta cttctcaaca attgcagcct ccatggcggc agtccgccaa cgcttcccca
    11941 tcaccaagag aagaaagggc ttttccggct gtgcctgccg gatgacgcgc aggcatctgg
    12001 aaaccagccg ctcatcaaaa gcaaaagcaa aataggcggt tacaggaaaa tcaaaaagcc
    12061 tgatcaggca ctccggaatc agctccgttc cgctgatttc cctctccgtc tttccgcagg
    12121 aacgggcaat attcaaagca attttcccga acaccagggc atgcacgaaa agctcgctcc
    12181 tctcaacagg ctcccggcag cagggttcct tcaatacata aaaacgcctg cggtcttctt
    12241 ccccggggtt ccctccgtac tcgtaaaaag gctcttcttc cggaggcaga gccaccactt
    12301 tcaaaggatt gccgatatcg tcggggttgt aatagtcacc ggatattccg gcaagtttac
    12361 agccgcatac ccagtgccc
//
LOCUS       DAWEMX010000072        12161 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2215_length_12161_cov_10.020486, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000072 DAWEMX010000000
VERSION     DAWEMX010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12161)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2215_length_12161_cov_10.020486"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3338..3347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3007 a         3504 c         3027 g         2613 t
ORIGIN      
        1 aaaaagtact tattgatgga cagcagcgtg tgactgccct catggccgct attgccggca
       61 aagaagttct ggatgaagac tttaataaag accgcatcaa aatagccttt aatcctcttg
      121 ccgaggatac aagcaagatt tttgcggtgc aggatgcttc ccatttaaaa gacaaacgat
      181 ggattccgga tatttccatt gtatttgata gcgactttga tgcgttcgaa ttctccgttg
      241 aatattgtac tatcaatgaa ggaataaagc caaacataat taataaggcc attgcttctt
      301 taagaggaat cgcgaacaga caaattggtg tgattgagct ggatgccgca ctggatattg
      361 atgaagtaac tgaaattttc attcgtatca attccaaggg gacgtccctc agtcaatcag
      421 actttgtcat gtccaaaatg gccgcagatg tagaacacgg aggcagcatg atgcgcaaaa
      481 cagtagacta cttctgccac ctagccgtga aacctgattt ttactcttca ttggtacgtg
      541 ataaggaatt caagacctcc aaatacgctg ataaaattaa atggctagcc catgattatg
      601 atgacattta cgatcccgat tatggagata tgctgcgggt atcatttatg caccagttcc
      661 gcagaggcaa acttgctgat ttggtcagcc tgttgtcggg aagagatttc gaaaccaagg
      721 aatatcggga tgatattgtg gcggaatcct accaaaaatt ggatgccggc attgtacaat
      781 tcatcaatca atacaatttt acacaatttg taatggcgat caagggtgcc ggtttcgtat
      841 ccagcaaaca gttgaattca caaatgacgc tggactttgc gtatactttg tatttgatgc
      901 tgcgaagtga tccaaccatt ccgaatgaac aaattaaacg gcatgttcaa aaatggtttg
      961 tcctttccac gctgacctca aggtatatcg gatctccaga aacccagatg gaccgtgaca
     1021 tgcgtaatat tgaagaaaag gggttcctga atttcaaaac agaagtggaa gcttcaacct
     1081 tatcagaaac attttggacg gttactttgc cccagaattt ggaaacgtct tccgtaaaca
     1141 gtcccgcctt caatactttc cttgccgctc aaattaacct aaattgcaat tccctgctga
     1201 tgaaagggac caaaatctcc gaccttatta ccatttcagg ggatgtacat cacatcttcc
     1261 cgcgtaatta tctgaaaaaa aacggcattg ataataagac gaaatataat caggtagcaa
     1321 attatattta tcttgatacg caggtaaata aagccattgg cgacgatcct ccctttgcct
     1381 attttagcaa agtacgagaa caatgcagaa caaaagctat cacactggga aatatttccg
     1441 atgaagaaac gctttactgc aatctagagg aaaatagcat ccctcaccat attattagca
     1501 tggatatcac tgattacgaa tcttttcttc aggaacgccg caaactaatg gcgaaattga
     1561 tgcaacggta ttatttggaa ttatagccat gctccaattg ataaaaccaa ttaagtatag
     1621 atgatatcct ccgtccgcac caccacatcg ccgggatcca gcatggaatt aatcagggaa
     1681 cagaagcgga atttgcctat atcttccgct cttacggaac attcctgaat gtcttcgcgg
     1741 gacagcagga aggcgcgctt ggttcccggg agcaggaaag tttccggagt aacccaggag
     1801 gctccgtccc cgaacaccac gttgctgtac cgcgtatccg tcaccatccc gttccggaga
     1861 atcagcgctt cttcgtcaca gcccaaggcc gcccctgccg ccaaaagccc tgtccgatcc
     1921 tcccatttgc atgagtaatc cagcccgggc atttccacac acgtcagcgt cttcacctgc
     1981 cggggacggt agggctcaaa ggaggtgcgg cgcacccttc cccgcgtatc atacgtgata
     2041 tggcatttgt aaactccacg gccttccggg ccgggacaag ccgaaagaac ggaagccaga
     2101 tcggggacgg aagcgccgtc cgaaccatga acgccgatgg cggcctccac cctccgctgg
     2161 tgccagggaa gcaggcaggg agcgccattc tcccatttaa cggtttcaaa aatcaaacgg
     2221 cacataaact ttggcaacag cttcccgata ttcctcttcc atccggctca tgacggtaat
     2281 tcctccaccg ctcttgtata ccattccccg ctcatcctcc tccatgaagc ggatggagac
     2341 ggcggaatcc aaatcccgcc cgttgaaaaa gccgaaaatt ccggtataaa accccctctc
     2401 catatcctcc gcttccgcga tggcccggca ggtggcttcc ttgggcgctc cggtcacgct
     2461 cccggcagga agcagcttca gaaggatttc ccccaggcgg gaacgccagt tttccggcaa
     2521 cagcccggaa atctcggaac tgcattgaag aatagtccct ttggacgttt ctacagggct
     2581 gatatagcgg taacgcttca ccctcacgcc ggttgccacc atggaaagat cattgcgcat
     2641 cagatccaca atggtggcgg attccctatt ctccttttca tccgtttcca gccagcggcg
     2701 cgcttcctgc gtagcggacg gaaccgttcc cttcatggga aacgtggaaa tggagcggcc
     2761 gcgcaccgtg acaaacggtt ccggagaaaa gcagacgcag ccgcggccgt gcactccgga
     2821 aatccgcgcg tccggcccca gcagcatccg gtaaggcgcg cgggcaaacc ggaacagatg
     2881 gcgcagcgtc aggttggttt ccacgggggt ggacacgcat aaattcagca ggtaggaatc
     2941 ccccctcatc agatggttcc ggacggaggc gaaggatgcc gcataccggg acacgggatg
     3001 gggggatgga atgaaccgca gccgttccgg aagcggaggg ccgccgtcct gacttcccat
     3061 cgtgcccagc ggcagggaaa aagcagccat gcgctccccc tccgtttccg caggttcaaa
     3121 caacaggtta tggcccatat catgggagat gacaaaaaag aacggacatt ccgcacggcc
     3181 cagggcattc atgcggctga tggtctgttc ccgtgtgtac attgtcggga attactctat
     3241 catgtgcgcg ccatgagtca accgaacgtc tggatcatcg accaccggga ttccttcacc
     3301 tggaatcttg cggaactcgt cagaatgacg ggaatggnnn nnnnnnnaat ggccgttccg
     3361 cgtgtggttt ccaatgaatc cccggaactg gaacaggcgg ttcaggcggg ggaaaagctt
     3421 atcctgtctc ccggtccggg aacggtggag gatgcctgcc accgggccac cttccgtctt
     3481 ctggaccgcc tgccccgctt taccccggtg ctgggcgtat gcctggggca tcagatcctg
     3541 ggcgtgcgct tcggagcccg tctggaacac ctttcccagc cccttcacgg agcgcgggaa
     3601 gtcatatgca ggacggagca gtgtcccctc ctggaaggat tgccggagca atttcctgcc
     3661 gcgctttacc attcctggcg ccttgccgct tttccctggc cggcggagct gattgtcacg
     3721 gcgagggatt ccgggaaaaa cgtgcaggcc atccgccaca ggcacctccc cctttacggc
     3781 attcagttcc atccggaatc catcctgacg ccggacggag aagcgctgct ccggaacttc
     3841 ctgtcccttc cggcaggcag cagggaaaaa cggcgcatca aatgattcaa ttccagccgc
     3901 ctggcatcag ggatgaacgg gacaagaccg gaaagtgtct cactcccgtg cggttccggt
     3961 tcccatgccc ggccgcttca gagataacgt ttttcacctt tatgaatgca caggacgaac
     4021 tcaagatcag ggacatggaa atcctgttcc agccctttca ctcacgcaag ctcagcactc
     4081 ccacccgcat cgtccttccg gccatgaccc ggggattttc tcccaacggg gttcccacgg
     4141 atcaggtagc cgcttattac aaaaggcggg ccagacatga agtagggctc atcatcacag
     4201 agggcacttt cattgacgaa cccagcgcct caccttcctc caactatccc aatttcttcg
     4261 ggggcgcttc cctgcgggga tggaaaaagg tgctggaagc cgtccacacc acgaattgca
     4321 aaatagcccc gcaactctgg catgtgggta tggcccggcc cttcaaggga gaaaacctgc
     4381 ccaatccgga gctaccgccc atcgggcctt ccggaattga cgtaaacacg ctggaacaga
     4441 cggcggaacc gatgagcatc gctaaaattg aggaggttat caatgctttt gcgagggcgg
     4501 ctgcggacgc caagcggctg ggttttgatg gtgtggaact gcatggagcg cacggctacc
     4561 tgattgacca gtttttctgg aaggagacca acaggcgcac ggacgaatac ggaggcgacc
     4621 tggtaggccg gacccgtttt gccagccgca tcatccatgc cgtccgcaag gcagtgggca
     4681 gccagttccc catcatcttc cgcttctccc aatggaaaac gggccattat aacgccaagc
     4741 tggcgtatac ccccatggaa ctggaagctt ttctggctcc gctgacggat gcgggcgtgg
     4801 atatctttga ctgttccacg cgccggtttt gggaaccgga gtttgaaggt tcccgcctca
     4861 atctggccgg ctggaccaga aagcttaccg gaaagcccac gatctccgtc ggctccgtgg
     4921 ggcttaaggg agattttacg aatgcctttg acggggggcc ggaagcggag gccgccagtg
     4981 tggaacctct ggcggaacgg ctgaaagcag gggaatttga cctgattgcc gtaggccgcg
     5041 ccctgctggc ggatgcggaa tgggcggaaa aaatacgcca tgcgcgggaa aaagatatcc
     5101 acctgtttac gaaggaagac ctgaaaacgc tgaaataacg gatttgcctg accgcaagga
     5161 tgatgcggga actcactccg caaagccggg agaggcaaaa acgccaggcc gctccaaaac
     5221 ggaggagccg tcttcttccc ccgtttccgc agcaatcatc attcccgaaa aggagattct
     5281 tttatttctt cctgacgcgg acagtcaggt acaacagccg cagaagagcc agtgccggaa
     5341 tctgaagcgt cagcaaatac cacggagcgg acccgaaata ttccagtacg gttcctgccg
     5401 gggcataccg ggtaaaaccg taattcgtat ccagccagat attcaccgga ataataaaaa
     5461 ccaggtaggc gtcgcacaaa cccagcgccc gcatgtcatc ccacggccgc gcacgccatt
     5521 gcaggacggc gggaaggtaa agagcggaaa tcaataaaag cccgtgcccg atgaaaaagg
     5581 cgaaaaaggc ggcggagggg aaaccgtcgt gaagcatggg agtaatcaat ccctggacgc
     5641 ttgccgtcag cacgccgaaa tacgccacgg cgcatgccca gggtgtacgg aaccacaggg
     5701 cgataaaaca gaccagagcc atcagactgc aaaaatgcag cggaagcctg tcctgccagg
     5761 gctcccaatg ttctctgacg atgcggccga atgtttccat caggaaagtc agcaggaaaa
     5821 agcttcccag aacctttccc acaagcaaac ggctctccat ccggagccgc cggcatcccc
     5881 acaggagccc tcctgctgcc agagccacga tgcccagggc agcccagtgc ggcaggctcc
     5941 aacggacaaa caggggcgca atttctccgg tcataaaaaa atccatcaac gggaaggagg
     6001 aggttccggg cacgcatgaa gtttggcgct gacagcctcc accttaagac gcagaacaca
     6061 tacggaacga accatgccgg gggtaaaatc cgcatccctg cccgtctggt gcagcatgat
     6121 gcggcgcaag gcatgcatct tttcctcatc ctcccggaga aattcgacga cgccacgccc
     6181 catgacgcag ccgtaggcgt aactgtaccg gcacgggaca tccccctcca gcaacacatg
     6241 atccccatcc atttcaaaag aaacctccgg acggttttgc aggatatcca gcttcctccc
     6301 ttccctggcg cagtggaaaa acaggctcag ggaaccattt ttttcttcat atccgaagtt
     6361 gaggggaact acatacacct ccctgccgtc ccacagtccc aggcggcaca ccttgaaccg
     6421 ggcgaccatt tccatcaaat ccccgtgccg ggtcacttcc ctgtcttttc ttctcataag
     6481 ccggcgccct ccatccattc attcatgcaa agggcggcac gtccggcgag cagggagcca
     6541 ttccgtacgg aagccgcccg aagtccgcaa atatccgttc ccgactgcat caattcacag
     6601 gcatggccgg tcagccactc ctccatccgg gaaacatccg cctccacatg gaactggaga
     6661 gccagggcat gcctccctat gcaaaaagcc tgatggggag tcacctccgt ggatgccagc
     6721 aggcgcgctc cggaagggac atcaaacgta tccccatgcc aatgcagaac gggcaccccc
     6781 tccagataac ggagagggga caaacgcccg tcccccgtca gggatacggt tccccaccct
     6841 atttccctgg cctttccggg ataaacgcgg ctgccgaggg catgggccat catttgagct
     6901 ccgaggcata cgcccagcaa aggctgccga ctttccagac gcatgcggac caaatccagt
     6961 tccgtcttca aatagggata cgattccgta tcccccaccc ctacgggacc gcccagcacc
     7021 acgcacaggt cagcctccag ccattcccgg acggaaggac agggagcgcc cgcccggatg
     7081 taccgtattc ggaagcccct gtccctgaaa agaggttcca gcgtaccgag atcttcaaaa
     7141 gccacatggc gcagggccag gcaagtgcgg gatgcagaac ggctactcat ggcgacggag
     7201 gatgaacctc aacaaggaat atagaaaaaa atcctccggg aaaagctttt tgccctcttc
     7261 cctccgcatt tccggaacca ggatgtccat catgaaacgg aaatatccgc cacatccgca
     7321 aaaaagccct ccgccgcacg gcataaatcc acaggattca attcaatctg gcaccccaga
     7381 cgtccgccgc tcaccaccat ctccggcagg gcttccgcgc tggcatcaat caccgtaggg
     7441 tatttccttt tcatccccaa agccgtgcag ccgccgcgca cgtaccctgt cacggcggta
     7501 atatccttca ccggaagcat ggaaagagcc ttttctccca cggtgcgggc agcctttttc
     7561 aaggaaagct ccgcgcctat gggtatcacg aacacgtaat actgccgcgg agctccctcc
     7621 gttaccagcg tcttgtacat cttctcctcc gccaggttca gtgcttccgc cgtatgccgc
     7681 gcatccacaa actcgcggca ttcataggaa taatgcctgt acgggatatg cagcgcatcc
     7741 agcaggcgca tggcatttgt ctttgcctcc ttctttttgg acataaactt gaaaagggaa
     7801 cgtcccggta acatagaaaa aaagccttcc tttggcaagg ccgagccccc caacgacccc
     7861 tatgaaaaag tggaacggaa acgggaattt ctctgaaaga gcggtaagga cagcaacgcc
     7921 gttctcccag tgcagcccgt tcacagcgga gaatttcaga aacggcggac ggcgaaaaag
     7981 aaaaaggaaa aaacgcctca tttcctcaga acccttgact tgccgctggc agaaaagata
     8041 acattcggta tcatggaaga ttatcgtgtc cggagaagcc ttggagaaca tgtcctgcgc
     8101 tgctccgtcc tgattgctgc gctcttcatc atgtctctgg gcatcgcttt gtccaccaag
     8161 gctgacctgg gggtctcccc catctcctgc acgccctatg tgctcagcct ggcattccct
     8221 ctaaccatgg ggaccgtcac catcctcatg cacctgagct ttgtggccgt gcaggcggcc
     8281 ctgttgaaaa ggcaattccg tccggctcat ctgctccaga ttcccatagc ttttatcttc
     8341 ggctttctga cggatttttc catgtggatc atggcgcctc tggaaccgga cggatacctg
     8401 tggtccgtta ttctctgcct gttcagttgc gtggtaatcg gattcggcgt ctttcttcag
     8461 gttaaggcgg attccgttct tctggcaggg gaaggcatga gcctggcctt cgtcaaactc
     8521 ttcaaatggg aattcggagc cgtaaaaacc gggatggact gcacgctcgt ctgcatcggc
     8581 ctggcctgtt ccctcatctt cctgcccgga ctgacaggca tacgggaagg aaccgtggtg
     8641 gccgccgtcc tggtgggaat gatcgtccgt tttttcaaca ggcacgtctt ctggccggac
     8701 aggctcctgg aacggctggc gcgccccgga gcagcaagcg agcttcctcc gctggcacaa
     8761 acggccgctt atgctccgga cgcccctctg gtcatttcca ttgaccggga atacggttcc
     8821 ggcggccatg ccatcgggaa aatgctggcg gaaaagctgg gcatccgatt ctacgactcg
     8881 gaactggtgt acctcacggc ctcccggagc ggcctcactc cggactacat ccgcaagcac
     8941 gaacaacagc tttccagccg ctttctgcac gaactttacg cccagaacta tgcctacacg
     9001 gcggaggaaa tgcctcctga agacgccacc ttcctggcac agagcaaagt catccgggac
     9061 attacggcca gtcaggcatg cgtcatcgtg ggccgctgcg ccaacttcat cctgaaaggg
     9121 agacccaacc tgttcagtgt tttccttcat gcggaccggg ccacgcgcat gcagcgcgtt
     9181 attgaaaact atggggtaga acctggcggg gcagcccggg ccatggacat catggactcc
     9241 cgccgccgca cccactgcct gcactacacc gggcaggaac tgggcaatgc acgcctctac
     9301 gacttgtgcg tcaacacgtc cgattacgga ctggaacgca cggtggaact gattctggaa
     9361 gccatcaata ccagaaccga acaatcttcc gctgtagaaa cggttcccgt ccgctccgca
     9421 tcttttcccg aaccggaaga ggacagcatt cccggagaaa tatccctggc ctgacagggg
     9481 caaatgtctc caacagccaa tacttatgta acaactgccc tcccggcacc ttgttcaccg
     9541 gtaacagaat ccgttgtctg aaaaaactgc ctggccgata aataggaaac tctctccccc
     9601 tttccataat ccccgcaaag ggaaagattc cgtatctcct tcagatgaaa aaccggctgg
     9661 aaaacgaatg gccccttcca cacaaaaggc tgcccctccc aactccccgt tttttgccgg
     9721 ctgcataatt caaaacaaca aaaggcagcc tttctgagtc caaaaaacat gagactctgg
     9781 tcactccacc cctcttacct ggatgctgtc ggcctggttg ccctgtggag ggaaggcctc
     9841 ctggcacgca aagttttgca ggggcagaca aaaggatata tccatcatcc tcagcttttc
     9901 cgcttccggg agacgggaaa ccccattcat gtcctggatt tctacctgaa aaccgtccat
     9961 gacgaatcca tccacagagg ctacaatttt gatttaagca aaatttctcc ctgcgaaaac
    10021 cgtccccctg cccttctcct gccggataaa cagctcgaat acgaattcct tcatcttctg
    10081 gacaagctca aggaaaggtc cccccggcaa tattccctgt tgcggcaaac cgcagtcccc
    10141 cttccccatc cgctttttca aataacgcca ggagatattt gctcctggga gaaaaagcct
    10201 gtttcgttat taacatgatt tcatttcctt ctccgggaaa ggctgccttc caataaatat
    10261 ttttggaatg cccccttatt cccgctggaa actattgatc cggtttggca tcttttcata
    10321 gtgacgggga taaaaccaca gacatacgcc atttccatct catcacccgg gcagtcactg
    10381 cagcgagcag aattattgca agaccctttc caacctttta cttcttcagg cccatacggt
    10441 gaaaaaacat gcccggcaaa ggaaaaggcc ttaaagcgtt tgtgctttaa ggcctttatt
    10501 tggtggagca tagcgggttc gaaccgctga cctcttcaat gccattgaag cgctctacca
    10561 actgagctaa tacccctttg actttcgtct tggtgcgtcc tgttgaggac ggggagaaat
    10621 tatcatttcc aaagcgggca tgcaagcttt ttctacaaaa aaattgcccc gggatgattt
    10681 ttccggaaaa tactgaatcc ggaaagggct gcaaatcaca tgcagacgaa aacagggaca
    10741 gtctgaacaa atgtgtttga atgagactca ttcctgtttt acctttccat aaatgatgaa
    10801 acgtcctctt tttactcatc gtatgatatt cgcctgcgct ctggcaattt tgtccctgtc
    10861 ctgtttcgtt cacgcccagg agactccagc tgcggaagga ccgccggccc agacggagga
    10921 cagcggcatt tccactcccc gcagagtcgc cggactgggc gttctggcat tcctgatcgg
    10981 ctggatgatt tggaggagca gttccagccg ccagaaggac ggaggccatg accaggaggc
    11041 atcccgggaa tgacgccgtt tttgaaaggt tgccctggtt cctgccgtag tcgttgcagg
    11101 aaaggagtct ccggccatga acattattct acccatcctg agctttctgt gcggtgtcat
    11161 gctgacggaa acagcttccg gctcttctga ggagggaaag ccttctcccg gcgtagtgca
    11221 ggaattgtgg ttcggcattc cgggggcatc cgtaaaggac ctgacccaca ggaagatttt
    11281 tgaaacggcg gccccggatg tccgcacgat cagccatctg gatgtggcaa acctgggaga
    11341 caggtacgga gcccgttact ccgccctgct gaaagtcccg gcaagcggga agtaccgcct
    11401 ttacctgtct tccgacgatt ccgcagagtt atggctgggc aaggatgcca cccagaagga
    11461 catggcctgc atcgccaccg tcaaaggata ttccgacatc cataattggg gcaaccagcc
    11521 caaccaggct tccgaacctg tccagctgga agctgaccgg ttttattttc ttcaggtgat
    11581 tcacaaggag gatggaggcc cggaccatat gtccgtcgcc tggtccgggc cgggcatccc
    11641 cgcccctgtc atcattcctg ccacggcgct gttcataccg cccggtatct tgccggaaga
    11701 aaaaaaacaa tccccagcca aagcggactg aaaggttttt tgattacctt cccgccgagg
    11761 agacatcatc aatttgccga cgagaaaata gatattttcc ataaaactcc atcaatcaat
    11821 catttccaat acgaaaaaaa accggacctc cctccatcgg ggaagcccgg ttttttctcg
    11881 tcgtatatca tgtagttaat tgaagcggcc tgacacagaa aaatgccaaa acaccttctg
    11941 aatccaaatc tagcggattt ctaatccgca accttcatca tgataaaaaa ttttcaggga
    12001 gagaagcttt tctcctctga gaagcaatgc tggtcattat gaattcaaag agaaagaaat
    12061 atttgaaaac aaatgattct cggattttga aacgacatga atagcaaaaa ttcctttttc
    12121 catcttatac ttcacttgaa aaagagagag gttttttact c
//
LOCUS       DAWEMX010000073        12071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2230_length_12071_cov_11.774384, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000073 DAWEMX010000000
VERSION     DAWEMX010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_12071_cov_11.774384"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2804..2813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8614..8725
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2449 a         3616 c         3463 g         2421 t
ORIGIN      
        1 cgccaataac ggcaatacct acacgcgctg ggtcacgaat ccggcccaca tcgaacagac
       61 gttccgcgcc caggaacttc tggaagccat ggcccagtcc atctgggaca acggggaacc
      121 cggcgtgcac aataacgacg tgatcaacct gtggaatccc gtcaaatcca tcggcaccat
      181 cactacgtcc aatccctgct cggaatacgt cttcctgaac aatacgagct gcaacctttc
      241 ctccttcaac gcctatcgct tcctgatcaa gggagaggac ggcaagcccg tttttgatgc
      301 ggacgccctg acccacgccg cccgcctggc catggtctgc gcggatctga atgtggaacg
      361 cggcggcttc ccgattgaag aaatcgcgga gggcacctac aagtaccgca ccacgggcat
      421 cggcttcgcc aacgtgggcg gctccctgat ggccctgggc gtgccttacg attccgacga
      481 aggccgctgg attgcctccc agctttgcag cgccctgacg gcggcctgct ggacggcctc
      541 cgcggaaatg ggcgcggaac tgggttccta cgtggaatac cccgccagca agaaagacct
      601 gctcgccgtg ctgcgcctgc acaacgcagc ccagaaactg gcaagcgccc tgccctccca
      661 gaaggactcc aagacgctgg acgacctgat tgagaacatc atcgccaatg ccggagggat
      721 cctgccggaa gcgcaggggc tcaccgcctc ctatgccctg catgcctacc tcaagagctt
      781 caaggccccc accatgatga acagggagcg catcgcccct gcggccaagc tggcggaagc
      841 cgcttccgac atgtgggcga aggtgatcaa ggccacggca caccgcaacg cgtttgtttc
      901 cgtgatggca cccacgggca ccatttccgc cccgctgggc tgctatgatg aaggcaccac
      961 ttccattgag ccggattaca cgctggtgaa atggaagcag ctggcgggcg gaggctccct
     1021 gaagatgttc aaccgcctgg ccctggaagg cctccgctcc ctgggttacc cggaagactt
     1081 cgtcaatgaa gccgctctgg aagtggcggg cctggacggc ctgatttccg cgttccaggg
     1141 gaatatggat tacgtcgtga accagctcat tgtggacccc tgcaatgacc aggccggccc
     1201 cgtgcgcctc gcgtggcgcc gcctgctctc cggaacggaa tcccgcagtg aaattcagga
     1261 aaaagtggct tatatcagca acccggccaa catggccgtg ctgacggcgg atgaactcgc
     1321 cgtagtcaac ggagccgccc atattgaatc cataccgtgg ctgaataaga aggacctgcc
     1381 ggtctttgac tgcgctgcca ccaacggcaa cggcatccgt gccatttccc ccgccggcca
     1441 tgtgctgatg ctgggcgccc tccagccctt catctccggc gcgtgctcca agacggtcaa
     1501 catgcccgtc tccgccagcg tgcaggatat ttacgattcc ctcatcatgt cccacgagct
     1561 gggtgtgaag tgcatcgcca tcttccgcgc cggctccaag gcgaatgccg tttacgtggt
     1621 ggataccccg gaaacgcgca tgttcaaggc caaccacgtc tgggaacagc tggtgaatgc
     1681 cggatcggac gccattgacg aaatcatcgc ggaggcatcc aagccgcgcc agcgcaagct
     1741 gcccggccgc cgtctgggcc agaccgtaaa attctccgta ggcggccagc tgacgggcta
     1801 cctgacggta ggcgtctatg cggacggcac ctgcggggaa gttttcggac gcctgggaca
     1861 agtcggctcc ttcgcctccg gcatgtttga agcttactgc aagctcctct ccaccgccct
     1921 tcagttcggc gtgcctctca aggaagtggt caagggtttc cgcaattact ccttcgagcc
     1981 ctccgggttc tgccgcgtgg gggacgacac ggaggcggac acctgcacgg aaatccgctc
     2041 ctgcgcttcc gtggtggatc tgattgccaa gattctggcg tggcttttcc cggaaagcaa
     2101 cggctaccgc ctgcgggacg tcttctccat ccccagcgtc agccttccgg gccaggctcc
     2161 ggatacgacg gtaagcgtgc tgcccccccg catcatcacc gctccggcgc atcccgctac
     2221 tccggccctg ccggaaattc ccgccggaaa ggttccggaa ggaacgatgc tcaacggcgc
     2281 ctccctctgc ccgcagtgcc actccctggc ctatgtccag gacggcaaat gcaaatcctg
     2341 ccgctcctgc ggatacaagg acggcggctg cggggaataa acggtaacca gatcattcca
     2401 tacccgctct ttccaggcgg aacccggcag ccgccgggtt ccgcctggtg aatttacgga
     2461 gaaggcattc tttccgggaa agacggtttt ccggccccct cccggcatga actttccgga
     2521 aagaagcgaa cggaaaccgc cgcgcgggaa aaggaatgat aggcgtttgc cttgaaatcc
     2581 ggcagcattt cattcatagt ccggcgcgta cgcctgaagc cgttcccaca ttttccatga
     2641 aaccctacta taccctggcc gatcttctgg atgaagaaca tccgtttgat gccggggaaa
     2701 ccgttcccgc cagactggcc gtaatcggtt ttcccattgc ccattccaaa tcccccgcca
     2761 tgcagcaggc cgcgctggat gcggcgggca ttcgcgcccg gtannnnnnn nnncccggta
     2821 tatccgcatc caggccgctc cggaagaatt cggggaggtg gtgcggctcc tcaggaaaag
     2881 gggattcacg ggggccaacg ttacggttcc ccacaagcag gcggcctgtt ccctgtgcag
     2941 tgacacagac gccctttccc gcgctaccgg ctccgtcaat actcttgttt tccaacagga
     3001 cggttccgtc tccggattca atacggacgg cccggggttc gcccgcgcca tccgggagga
     3061 attttccgtg gatttgcgcg atttgaaagt ggcccttctg ggctcctgcg gcggggccgg
     3121 gcttgccctg gcctacacct gcgccatgca gcgttgcgaa cggctgacgc tggccggaag
     3181 atcggaagaa aagcttcagg agcttaaaaa caggctgagt tcctttttca ttgacgaaca
     3241 caggctggaa ggggcctccg accgcctggc ggcccaccag aacaatacgc cccgtttcaa
     3301 tgccgccgtg gaggaagcgg acctgatcgt caacgccacc tccctcggcc tgaagcctac
     3361 ggacccttcc cccgtccccc ctgcactgct ttccgcccac catctggttt atgatttaca
     3421 gacgcatgac gatgccttcc agatggaagc ccgctttcag ggcgccagag tttccaacgg
     3481 cctttccatg ctggttcacc agggggccct ttcctttgaa ctctggttcg gcatcaagcc
     3541 ggatatttcc gccatgcgcc aggctcttga acaaaagcat gactgacggc accccccatt
     3601 ttaacatcca tgctttccgg aacgcgctgg tagaatggtt caggcgcgaa gggagggatt
     3661 acccgtggcg gcggacaacg gatccgtggc acatccttgt ttccgagctg atgctgcagc
     3721 agactaccat tcccaccgtt ttgggaagat atgacagatg gatgcgccag ttccccactc
     3781 cggcgcatct ggctgccgtg gacgaacaga cggccctgcg ctcctgggaa gggttgggct
     3841 attaccgccg cgtgcgttcc ctccaggcca tcgccaggga aatcgtcaac gaattcggag
     3901 ggcggttccc ggacaatgcg gaagggctga aacgcctgcc gggcatcggc ccctacacgt
     3961 cgggagcgct cctctccttc gccttcaaca aggcggctcc cattgtagac gccaatgtcg
     4021 cgcgcgtcct ggcccgcatt gacaattatt ccgttcccgt ggattccacg gaaggccaga
     4081 aatacctgtg gagccgcgcg gaaagcctgg tggatccgga acatgcccgt gaattcaatt
     4141 cagccatcat ggaacttggg caaacctgct gcagcatcag ttctccggac tgcctgctgt
     4201 gccccgtgcg ccccttctgc tctgcggaac ggccggaaac gcttcccgtc aaaaatccca
     4261 agcctcaagt cacccgggtg gaacaccacg atattcttta catccgcggc aaatccgtcc
     4321 tgctggccaa atgcccggaa ggcaagcgcc atgccggcat gtaccgcttc ccccagaggg
     4381 aggacgagca caccctttcc ctgccccatg tcctgaaaca aacctacagc attacccgct
     4441 acagggtgac ccgctacatc caccatgtga cggatacgcc tctcctcagg gaaggagagg
     4501 aattcgtgcc gttggacaaa atccacgggc tgcccatggc ctcaccggac cgcaaggcac
     4561 tgaactcccc caccctcggc aaactgctgg accatatcag atgaatgaca gattgacgcc
     4621 ggaacttgtc ctctccgcct attgccaggg gtgttttccc atggcggacc cggaaacggg
     4681 agaaatctcc ttttacgaac cggacccgcg cgccctgatt ccgctggacg accgcttcca
     4741 catcccccac ggcctcaaac gggctttgaa taaaaaaccc tttgaactcc ggatggatac
     4801 cgcttttccg gaggtggtcc atgcctgcgc ccggacggac cagccggaag aacaatggat
     4861 agacggacag attgaagaag catacggcaa gcttcacgag atgggcttcg cccacagtgt
     4921 ggaatgctgg gacgaggaag ggctgcaggg aggactgtac ggagtagccc tggggaaggc
     4981 ttttttcggg gaaagcatgt tccaccggaa aacggacgcc agcaagatcg ccctggtggc
     5041 tctggtgcaa tacctgcggg cgcatcggtt cctttttctg gatacccagt ggactacgcc
     5101 gcatctgctg aaattcggaa cctatgaagt gccggccaag gaataccgga agcttctgaa
     5161 gagggcgctg gaggaacaat aaactatatt ttactgtccg gtgtaaacgg tcatgagatt
     5221 gattcccaat tatgggagat ccgacagcgc accggatttc ataacctgct gatgattttt
     5281 cttcgggctt tgccatcaat acttgccccc catgccaccc cttacgacca aacgttcagg
     5341 aagcatggct ccggcataaa aaactccgga atcgacatca acggcactcc gggaaagact
     5401 gcggagtatt ttgggaaaga tgcacttcct cttccgccag gagagcttcg gcccatgatg
     5461 ccattaacat gattttttgc agcaaggcgg aagccgtcaa acatgcccaa accaaacgca
     5521 tggagcagtt tggcccgcaa ggttcaagcc atatctgaca agaccgttcc atccgctaaa
     5581 aaagcccgtg gttccgaaga accacgggct gtttattaac ccttaacaaa aaggtttatt
     5641 cctgttccag agcagcctgg gccgcggcca gacgggcggt cgggacgcgg tagggcgagc
     5701 agctcacata agtcaggccc agcgtgttgc agaacttgac ggaagcgggg tcgccgccgt
     5761 gttcgccgca gatgcccagt ttgatgtccg ggcgggtgga gcggccgagt tccacggcaa
     5821 tcttcatcag cttgccgacg ccgttctggt caatggaagc gaagacgttc ttcttgatga
     5881 tttccagttc ctggtactgc ggcaggaagg agccggagtc gtcgcggctc atgcccaggc
     5941 ccgtctgggt caggtcattg gtgccgaagg agaagaactg ggcggtttcc gcgatttcgt
     6001 cagccgtgac ggcagcgcgc gggatttcaa tcatggtgcc gacgaggtag tccactttga
     6061 cgcctttttc ctcaaatacc tgctgggcca ccttgtcaat gatggctttc tggatgtcca
     6121 gttccttctt gaaggccacc agaggaacca tgatttcagg caccacggca aatccttctt
     6181 cctgggagac ttccacagcg gcttcaaaga tggcgcgggc ctgcatttcc gtgatttccg
     6241 gataggcgat gccgagacgg cagccgcggt ggccgagcat cgggttgaac tcatggagtt
     6301 cggacacgcg gcgcatgata acttccacag gcatgccgat tttctgggcc aggtcaagct
     6361 gctgttcctt cgtctgggga aggaattcgt gcaggggcgg gtccagaagg cggatggtgc
     6421 ccggcaggcc cttgagcgcc ttgaagatgc ccttgaagtc ccccttctgg tagggaagga
     6481 gcttggaaac ggcggctacg cggtcgctct tcttggtgga gaggatcatt tcacggacga
     6541 agtcgatccg gtccccttca aagaacatgt gttccgtgcg gcagaggccg atgccggtgg
     6601 cgccgaaggc gatggcggtt tccgtctgct tgggagagtc cgcattggtg cgcactttca
     6661 ttttggtaca gtcgtcgcac cactgcatga gcttggcgaa gttctggtag gtgcggctgt
     6721 cttccggctt catggtcttg gaaatcagga cctggatgat ttcagagggg gaagtttcca
     6781 ccttgccggc gtataccagg ccgctcgttc cgtcaatgga gaggtagtca ccttcgctga
     6841 tggttacgtc gccaacggaa acggtgcggt tgttgtagtc cacaatgatt tcatccgcgc
     6901 cgcaaacgca gactttgccc atctggcggg caacgagggc ggcatgggag gaaacgccgc
     6961 cgcgggcggt caggatgcct tcagcggcaa tcatgccgcg caggtcttcc ggagaggttt
     7021 ccagacggac cagcagaacc ttttcacctc tgtccgcggc ttccacacag cgttccgcgt
     7081 tcaggtagat gcgtccggtg gcggcgccgg gcccggcggg aagaccgcgg gtcagaacct
     7141 tggcactctt gatggcttca cggtcaaaga tgggggtcag cagctggtcc acctggtccg
     7201 caggaatgcg gcgcacggcg gttttccagt caatcaggcc ctgttccacc atttcacagg
     7261 caatgcggaa agcggccacg ccggtgcgct ttccgttgcg ggtctggagc atgtagacgg
     7321 tgcggtcctg aatggtgaat tcaaagtcct gcatgtcatg gaagtggttt tccagcactt
     7381 cgcgaatgcg caccagttca ttgaaggcgg cgggcatcac ttcatgaagg gcagcaacgg
     7441 gggcgggcgt acgcacgccg gccacgacgt cttcaccctg ggcgttcatc aggaattcgc
     7501 catagaattc gttttcgccg gaggcggggt tgcgggtgaa cgccacgccg gaaccggatt
     7561 cctcacccgt gttgccgaag accatggcct gcacgttgac ggcggtgccc cattcggacg
     7621 gaatgccgta tttctggcgg tacacgatgg cgcggtcatt gttccaggaa ccgaacacgg
     7681 cgccaatggc tccctgaagc tgttcccagg ggcattccgg gaattcatgg ccggtgcgtt
     7741 ccagcaccag ggccttgaag cgggagacaa gttccttcag gtcatcggca gagagcttgg
     7801 tgtcttccac gtcctcgctg ccaaagcgtt ctttcttgat ggcagcgatg gcggtttcaa
     7861 agggttcgtg gtcttcatcc ggggttttct gcacacccag caccacgtcg ccgtacatct
     7921 gcacgaagcg gcggtagcag tcccaggcga agcgggggtt gttcgtagcc ttcaccatgg
     7981 cttccacgga tttttcattc aggccgaggt tcaaaatcgt gtccatcatg ccgggcatgg
     8041 attcacgggc gccggaacgc acggccacca gcaggggcat agcttccgta tcgccgaact
     8101 tgcgtcccag gatctgttcc attttggcaa tgccttcctt gacctgcgcg tcaagctggg
     8161 cgggataagt gcagccgtga tcgtagtagt aagtgcaaac ttccgtagtg atggtgaatc
     8221 cgggagggac aggaaggccg atacgggcca tttccgcaag gttggcgccc ttgccgccca
     8281 aaagattttt catgctgcca ttgccgtcgg cggaccctcc gccgaaagaa tagacgaatt
     8341 tgtcagtaga tgcgctacac gcgcaggaat tggtggacat atcgattatt gtagtcgaat
     8401 taatgattct gcccaaacat cgaccaaagg agccaacaaa agacccaata gcgagttagg
     8461 gtcgaataag gatgccttcg gctccttgct ttgtcaattc gaaattgtac ggaaaacaaa
     8521 aatatttttc agagtttttg gggaaaagaa tgcatgccaa aaccctgctt ttccagcctg
     8581 tcccgtccgg aaccgctctt cctccgggca acgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnncccga aattacctgg aaagaaaagc gcagcgagtt
     8761 tcttttcatg gctgtccgga aaagaaaaac ggacgcttat caaccggata agcgtccgcc
     8821 ggaaataata tgccctgttt tttatgcctt gcagaaggtg tcttcatccg cacagtcagc
     8881 ctgaacccac ttgacggagg aatcaccgga agctatatgc acgaggcaat ggtacacggc
     8941 ttcctggtcc gcatccagcc gggcagccat ctgggcggcg gtttccgggg cggttcccag
     9001 agcccccctt accctgccca gcagggccag gaaggaaccc gccgcttttt tgccggcttc
     9061 cacgccgggc tggtggtagg cgttgatgtt gaccagggaa gcatagaagg agacggcgcg
     9121 ctcaaacaga gcaatcagca tgccaagcgt tttggcgttc acctccggga tggaaatggt
     9181 gatggaggaa cggccggatt ccgccagagc ctggcgggtg ccgcgcagga agccctggag
     9241 gaagtctccg caggtggcgc ccgcttccac ttccaccgca tccgcggaac attcgcgcac
     9301 ttcaatgaag gtggcgaaga agttgttcac gccgtcacgg agctgctgca cataggcatg
     9361 ctggtccgtg gagcccttgt tgccgtacac ggaaatgccc tggcagactt cgttgccctc
     9421 caggtccagc cgcttgccga gggattccat aatgagctgc tggaggtatt tggagaagag
     9481 caccagggca tccttataag ggagaaccac catatccttt tcccccttgc cgccggtggc
     9541 cttgtaccag gccagcgcca gtttcatgga ggcgttgcat ttcacgcagt cccggcgggt
     9601 cttttcgtcc atcaggcgcg cgccttccag cagatcatcc acgttcacgc cctgaagcac
     9661 ggcgggaacc agccctacca cggacatgac ggaggtgcgg ccgcccaccc agtcttccat
     9721 cgggaaacgc ctgagccagc cttccgcctc agccgttttg tccagccggg aacccgttcc
     9781 cgtgacggcg accgcctggg gtgcgaattc cagcccgttc ttcctgaaga agtcctgggc
     9841 caccagcatg ccgttgcggg tttccggcgt gccgccagat ttggaaatga caatgacaag
     9901 cgtttcttcc agagggaggg attccagcac ccggtacatg ccgtccggat ccgtgttgtc
     9961 cagaaagaac atgtccagcc ctgccgccgg aatggcatcc gccaccagct ccgggccaag
    10021 agcggaaccg ccgatgccga tgacgagggc ggcggaatat tgttccgcgc ggggaggcgt
    10081 tattttgccg tccagaacgt cccgtccgaa ggctttgacg tccgccaggg ggccgtcaat
    10141 ggcggctttc aattccggcg tgggagccag ggaggagttg cgcagccagt agtgccccac
    10201 catgcgtttt tcatccggat tggcgatggc gccggattcc agcgccttca tgtcggcaaa
    10261 cgccctgtct atccgtccct gcatggaaga caggaatgcg tcgtccagcg gaagttttga
    10321 aaaatcaagg gaaatgccca gttcagggta gcgaacgagt tgttttgcgt ataagctcat
    10381 gatggtcatg gattggaaaa agggttaccg gagttttttc aggatgcttt tggcctgcac
    10441 gtcgccctgg tccgccgctt tctggaacca ttcccgggct ttggccatgt ccttgggcag
    10501 gccgaaaccg gtctgataca tcacgcccag gttgtattgc tccgaggcat agccttccgc
    10561 agccgcccgg cggcgcgtgc gggactgttc cctggcttcc gcagcggtaa ggcccagaac
    10621 ccgcgccgcg tccggccacg cggctccgaa tctggaagcg gaaggggcat agccggcggc
    10681 tccggccttg cggtgcattt cctccgcttt tttcttatcc ctcttcacgc cgtatttgcc
    10741 ctggtcatac aggaacgcca tgccccagta gccacggggg cagcccagct ccgccgcctg
    10801 ctggaagcat tcaaaggcat ctttatattt tttggaaccc atggcgcgca cggcgtcgga
    10861 aatatggagt tccgcctcct tcaagcttgg gaattccgga atagccgccc attccctttt
    10921 cagttcctcc ctgtcccttt tggacttggg aggcatgccg gacggaccgg aaaaacgcaa
    10981 aagggggaag taaccctcca tggctcccat gttgcccagg ccagacatca gaaaaacgcc
    11041 gggagagacc ttccacatgt gcatgtactg aatgcggcct tcctgaaggg attccctggc
    11101 cacccactcc ggcatgttcg ccggttcccc gaattgttcc gtcaggccca ccagaagagc
    11161 cttataggcg gatttcagcc tggtttcata ttcggaacca cggtatttgc tccgggagta
    11221 gaggacaacg gccctcaagc cgccggaggg gtcatagtca aagttcaggg aaaaagtctg
    11281 gccgcctatg gactgcctgg ttttaaacac gccgttgagc ccggtgcgtg aaaggagggc
    11341 gtccgtgggc ggcccttcaa gctgcttgct ccgcctcagg gttttcagca cctggtcctt
    11401 gtccatcccc acctccagtc cctcataaag ggcggcaagc aaaggggaac atgtgagagc
    11461 gaagagtcca gcaacatagg ctagtttttt catagaaaaa gcaattgggc ggttttctac
    11521 cagaccggaa gagttttcaa aaggataaaa tgcgtcagcg atggagagga aaatttttca
    11581 taaaatattc atttccagtc tacccacagt cttttcaaat aatcctcccc ggagaaatcc
    11641 gggggcatgg ggtcatgagt caggaggaaa ccgggaacgg cacgggccac catcctgcgg
    11701 aagtcctcgt ggcccagctt gcggcagttg ctggtgcaca gcatccagcc gcccggttcc
    11761 aggcattcgc gagccagggc caccagctcc ccgtagtggg attccacccg gaacaccttt
    11821 ccgcggtcat cccgggaaaa cgtgggaggg tccagaataa tgccgtgaaa ggtgcggccc
    11881 tgcctggcga accgttccag ccagcggcgc gcgtccccct tgcagaaata ctgggcggag
    11941 gggtcaatgc cgttcagcat gaaattttcc ctggcccagt ccaggcacgg ctgcgccaga
    12001 tccagcgtgg ttgtctgcgc tccgcccagg gccgcataaa cggaaaaggc tcccggatag
    12061 gcaaaggtat t
//
LOCUS       DAWEMX010000074        11979 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2257_length_11979_cov_10.166219, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000074 DAWEMX010000000
VERSION     DAWEMX010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11979)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_11979_cov_10.166219"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3013 a         2767 c         3032 g         3167 t
ORIGIN      
        1 cgtccacggt tcctcccgtg ccggcgacgg tttccagggc cgtccagatt tgctggtgcg
       61 ccgggaggct gaatgtctgg cggttgatgc ccttgtcccg gaggtccgcc aatgcctggg
      121 cgccgtccat tgcccgggag agtaccagtt tttcggcgtc aatgattgtc tgcgagtcga
      181 tcatgttgtt gaagttgttg ggggttagag ttcctgaagg ttcgaatagg ggtctccgtt
      241 ttcaggaggc ggcggatggt tgagggcgta gctggtggcg aagctgatgg cgtcggattg
      301 ccatttggtc acggggatgc cgttgcgggt ccagttgacg gcgtccctac ttccccagta
      361 ggccgtggcg cagtccggta tctggtcggg agccaaacgc acacgccccg caaaagccgc
      421 ggcctgcaaa tgagcttcga cttcctccac ggtgcatgga gagggggtaa gggggtgaat
      481 tccttccttc ccttccttcc ttacggtttc ttcatgggtt atgtctgggt tattaaaaaa
      541 aaccgactgg gtttcttctg ggttttcgga aataactgac gttggttttt cgtgggtttc
      601 cttttcggtt tctacactgg ttccaatgtc ggttttcctg ggtctccccc caagttttcc
      661 attttcacgg gcggtcttcc ggcgcgtttg cacgcttgcc tgaatttcat gcggatagcc
      721 gaatacgacg agattgtcgc cgtcaaagtg gtagagttcg ttttccacgc tgatttcctg
      781 atccgtcacg ccgcaggtct gcatccagcg gcgcatgccc caggagcggc agccctcaat
      841 aatgccgccg ttttcctgtt cgcagcacca ggccagcaga gagatccagg tggcgcgctg
      901 tatgggttcc gccccgatat attcggggct ggaaaacaag gctgttggga tattgatgaa
      961 ttccataata aaaaagcgtc agttgggggt tgtagttcat ccacaggatt caatcttctt
     1021 gccggcttcc tttcctaagg atttgcctct tttcccaagc atccaaaatc aaatgagcat
     1081 gctttgcggc gagcgccagc tctccaagat gatatttttt tccggtcagg ggcgctctgc
     1141 gcagctcgtg cctccacggc atataacaca agcactcata gtcggcatac tcccggcgga
     1201 attccgcaag tcttttgtat gccttccaat agccgatgcg cgccttttgg catggcgtcc
     1261 agggcatgat cttcatgcga acctcctttc caaaatagat gcctgttccg gggtaagata
     1321 ctgccagctc tgcggcggac gggtcaggcc aatggcagag agtggtacag catgagggag
     1381 ccgcacggga tcctgaacgc cccaggcaaa gcaagggaga taggtacgca ggtgagtttt
     1441 agagacgcaa gcatctttga tgaataatcc aagagctttc ccgaatggca tgtgggttat
     1501 gagcgcaaag gaaaccatcc ggcatttgcc aatgattgtc cgctctccat ccttgccgga
     1561 ttcgtaaagc caaagagtga cgtgctctcg tttcatgaca cggggcacat tttgacgtag
     1621 ttcccatgtc ttttgcccgt ccatgataag accggagaaa ggccgcctga cggataatag
     1681 gatgttaatc attactggcc tcctttccgt caatgatggc ccttaattca tcaaataccc
     1741 gtagacgtgt atgattcctt gtcgtgcaaa taaggtgctg cgcccaccgg gcatgccgtt
     1801 tcgtggggta ctccatgcga tagcgggcga taatcccttt gtggtgcaca atcgcggcct
     1861 gaacttcata tttcccatcg tcggttttct tcatggggca gacctgctgg acgatgatgt
     1921 gagggttccg tttcatggct ctgatccttc ctgcacggtg attgttattt gcggctcttc
     1981 accccaccat ttatccacgc ttgcggaata gacctgggcg tcatcttccc aaaatctcaa
     2041 ccgggtcatg acatcctgca gggttttggc caggttgtcc cagtccggtt tggtcgtttt
     2101 cggaatgagc ccgatccgtt tttttttcgg ctcgcttttg cggtagggcc agacgaaagc
     2161 cagtttcagg gagaccggcc ccgtcagggg ccgggccggt tgataaggtt tcagcagagt
     2221 caggtaatcg ctgatgacca gtttcaattc tttcgtgtcc gccagtttgg cgtgttcccc
     2281 gatatggacg atttttttgt tctggtgcgt tttcgtcggg ggaacgatcg gcagcatgat
     2341 ggtcatcggc ttgttcatga cgctttcgaa ttcctttcta tttcccgggc ctgggattcg
     2401 gcgtcgaagt ccagcttcag ctggccgtcg tcctcaaacc aggcattcgc cacagctgtt
     2461 ttcttgatgg agcctgacac tttcacacag actttctgtt ccccgtcagg gattttgaca
     2521 ctgatggaga ggctgaaatc ggattcgtct tccgccgcct ggcgcctgat gtcttcgtag
     2581 tcgtcgaatt catcaaaggc catgcggacg gcttccagga tggtttcttt ttcgtgttcc
     2641 gttcgcgcgc tcatcattgt tgattagaag gggatttcgt cttcttccgc cggcggtccc
     2701 gccgtggcgc tcatgtggtt gttggccggc agatccgccg ggcgcggagg tagggacgcg
     2761 ctgccgcgcc ccgccgctac cctgtcctgc gccgccatga tggcccgggc ttcgtccggc
     2821 cccagcacgt cttcgcagtt gctgaattcg ggataactcc cgtccgccct gggcttgtct
     2881 ccccgtttga cgctgagccg gacgtagcag ggcttgccga ggtattccgc cgggttgatg
     2941 atgacctgct ggcctgcctc aaataccttc ccggttacgt ttttgacgaa caggtcgatt
     3001 ttccaggcca ggtctttcga ggcggtcagg tagtgacgca ccgtcgccgc cccttcaggg
     3061 ccaaaggccc tgatgtggac agccagctgc gggcatcccc gcgttttggc tccctgggaa
     3121 attccttctt ccatcttgac gatttttcct tcatagacgc ccgcggggag gaatccgtat
     3181 tcgccgggct cgccttctga aatataactg aacataatgg ttatttgttg gttgtgattt
     3241 tggagacgga gatttttttg acgtaggagg atccggcccc cgtcctgacc agttcttccg
     3301 ggaattgttg ttccggcagg gcttccgcga acagggcgcg gaagatgtcc gccttgagcg
     3361 ggccatagga tttcaggagt ttcggcacgc caatccaggt ggcgtatttg gccacgtctt
     3421 ccggagcgac ggtgtccgtg cctttccggg agacgcgcct gaatccgggg acttccgttc
     3481 cgttgttgag gtagtcgagg attttttctt ttcctttttt gacataggat tccaggattc
     3541 cggccttggt gacgaattcc gccagcctgg aagggttttc tgcgatttcg gcaaagctcg
     3601 cttccagcgt tccggcttcc gccagggaca gcatttcctg cgccgcccgg ttccgcagtg
     3661 gacaggtgtc ctgcgaggcg caccagccgc agtagtcgca gaggcgcggc ccaccgccgc
     3721 gatccacgga gtccaccacg tcgttgacga tggagattgc ttcccggtag gtgaatttcc
     3781 gggtgacgat ttgctgctgg tcgcagaaga ggaggtggca ggtgatttca tccaggaatt
     3841 cccgttccat gaaggatttc gcgtaagagg cctgctgttc ccagtagttg cggatttggc
     3901 cgcttttgag gtcgatgagt ttgcccagcg cggggcagag gcagtccgct tccccgcctg
     3961 tcactcgggg gtgccattgc gggaaggcgc agcggttttt gtcggcaatg acctcttctc
     4021 cggagcagag cgtccggacc gttttcactg cccaaaggat ggatttcttt tcatcggctt
     4081 gcaggtgttc acacgccctg aattcgtcca cgcccatgag caggtcccgg aaggcggcgt
     4141 ccatccgggt tccccgctgg gccgcttccc ccgcgtcggg ggaggagatg aagcagggac
     4201 attgcgccag cttggggagc agggacggcc tcaataattc cgtggacggt gccggacgga
     4261 gtccggcaat gtcagagagg attttttgca ggtcgtccag gttgacggcg tattccgctc
     4321 cgtccaggga gaggacggca tgcccggttt cgcgggcgac gttgatgcag gtgacgggtt
     4381 tcatcgggcg gtcggggtgt tgtgctgcag aacggccgtg ttgaaccggt cgggggcgga
     4441 gaggatgaag gaggcgaatt tttccgagac ggcttcaagg ccctgccccg gctggatttc
     4501 ctttttgtac gcgaggaagt tcagcgctcc cggcacgtcg ttgatgaccg cggccagttg
     4561 gtccgccagg gagggatccg gtttttcctg ttgtgcggga agaggctcct gtttttcgcc
     4621 ggccggagcg ttcccggaaa attcgcagcc cgttccaaac aacaggcggg agatttcccc
     4681 ggcgtccatc gccatgaccg caggcatccc gtgccggttt ttggcttccc aggtggcccg
     4741 gtgctccgtg tagacggcac gcagttctcc gcctttggcc ttgcctccgt ccgtgaaggt
     4801 agtcacgtag ttgcagaaca ggatggcgtc cccccattcc ttgagttttt ccttggcggt
     4861 gatggcctgt ttggccggag cgttgatttt gatggtgtac atggtgtagg cttccccttc
     4921 cggagggttc accgtttcca cgcggcagtg gcagatgacg gcaatgtgca gccccgcgtt
     4981 ccggcagttg tcaaagacgg aaagcaggtt gacgaacatt tcgctggcct gggcgtaccc
     5041 tttcccatag ccgatgcttt caatggagga gatttttccg tctttggggg aggcgttgta
     5101 gtccctgatg acctggcggg cgcacatgtc ccacaggcgg tctcccgtgt cgacgacgag
     5161 cgtccggtag gggaggcttc cgttccgggc ttccttgtag atgtcctgca gggcttccag
     5221 catggcgccg tagtggtcta cctggatacg gtccacattc atgtgctggg ttccttcttc
     5281 cgtgtccagg aagagggggg cgggcagccc ggccgccagc gtggattttc ccacgccttc
     5341 cggcccgtag atgatgacac gctgcggccg ctgctgcact ccgcgcttga tgttttgtaa
     5401 tagattcata ttatttcctt gtttgattgt attcaggtcg ggtgtcagtt cctgctggcc
     5461 ccggccatct tttttcaggt agttagagat ttgcattgaa cgaaccggtt ttgcggtagc
     5521 gctttccggt cgttgtatca gagagttgtt caataatggc ggttgtaacc cgattcaggt
     5581 ccacgtaaag cactccgcca catttgacaa tcggaagcag ttttttaccg tcggcgcttt
     5641 ccaacatggc ccggctcggt tcagaacctt tggcaaatag tccgcattgt gcaaacttgg
     5701 agagccttac cagccgggac ggcaacgggg gagcagtgat attgatgttg atttgaggtg
     5761 caccggcaaa tgtggcatcg gcgtttttag gccatgattg agacaggaca tcagctaatc
     5821 cttttgaaat gtctctgacc acttgttcag gcaacctatg gtcttggttc ataattactt
     5881 aatgttaatg taaataaaat aaacaatcgt gaaggcacca accagcagag cggaaaagac
     5941 aaggttctgt acgataccgg gccggggctt gagagcgttt tccggaaagt tcaacgggca
     6001 accgtaaaga acctccattt tcgaggcacg gtcgcggcgc atccagtatt gttcgttcgt
     6061 catttttttc attgttgatt tcttgtttat ctcgttttag tttgttaaag ttatgcgttc
     6121 gaaaatagaa ttttattctt atcgctgggt tgagcctaaa gaagttttcg gaattcacgg
     6181 atattggcgg ccggatgaag ttatctgtgc tttcctcgac caaatacgaa tagaaccgcc
     6241 aagagggcag gaaacaaccg tggcaaccat ttcaacagga acccccgatg gacaagagca
     6301 atacattgtt cctgcttttg agtatgagag aataaagcat gcccaatacg gacacgggca
     6361 tgccctcccc ttctattcaa tacagtggga tccctctgca ccagtttaag cgtccaggct
     6421 ggcgcatctg tgcagtaagt cccctgagga atcaacagga ctctatcaag cctggcctga
     6481 tagacctcat acaacatgga atcatcttcg taggtgaact gtacaatatc gcccggaaga
     6541 aaagggtttt cgggggacgg cttgttttca aaaccatcgt agactgtttc taataatttc
     6601 ttttgcatgg tatttatcgt ttgttaaacc gggttaaagc tcgtgccagc cgagcagctt
     6661 caattcttcg atcagagctt cttccattag gctgccggct tcttggggtt cttcgggcgg
     6721 gggagatcgc gggccgtggt cagatgaaca cggaatcccc tgcggaaaga ttctcgttca
     6781 atgatgcgga taatagcctc cgaaggcttc aacccttctt ccgcttcggc cagcagatgg
     6841 ctcttgcagc cgtccggtaa tttgtctaag tcgatttctg ttttcatgat ctctcgtagt
     6901 tgatgaattc tttttacgca tacatcatga atgcgtcaac acgtatttca tgatttatgc
     6961 gtgatatttt ctaatcaata ggttgacttt tcatgaaatg tgcgtaaaat caacgtatga
     7021 cgccgactaa agaagacgta aaaaaatggc tcaaaactat aggcaaagat cgcttttggc
     7081 tgtcgggaaa actcaacacg cctaaaagaa ccatagacaa ctggcttgct cccggagggg
     7141 cattccctgc ttatgcagtt cttcagctcc aaaaactaat gaacggagaa gccgaatcct
     7201 ctccccgcat cgtcatcgac ttcacagatg aagaatggga catcatctgt gaggccgcta
     7261 aagcccacaa agaaaccttc ttggaattcg tcaataccgc cattcaaaac gccgccaaag
     7321 aaaaagaagc cgctcgtaag aaatttaccc cagtagaaac atttacagaa ccagtcttgg
     7381 aagcccaggg acgaatcatc ggcaacattg ccgccggcaa cctggcggat ggagacacca
     7441 tcccgcagga catccggcta taccgtgaac tggaaaaagg ggaatacctg ctgcgcgtga
     7501 acggccactc tatggaacct tccattccgg acggctccgt agtcatcatg aaaaaataca
     7561 ccatcccccc tatccccaaa cccggaacca ttgttcaata ccatgatgaa cgcggggtga
     7621 cgctcaaaaa actggtccgc aggaaaaacc cggaaaccgg taaaatggaa tacaccctcc
     7681 atcccatcaa ccctgccttc ggagacatcg aacccatgga cggcggcaaa atctccgctc
     7741 tatacgtgga aaccctggac aggtgggaga aagcttgaat atttttattt aataaatttt
     7801 cagtatatta ttattccaag aaatgactat gagaaaaaac aggattgcca ttatccccgt
     7861 ttcatattag cttggtgaag gatatgtcgt ctaagtttac aagacctaaa ctcaaagcca
     7921 tagatttttt ctgtggcggc ggaggtatga cttgtggact tcgacaagct ggaatcgacg
     7981 ttttggcggg agtggataac aatctcaaat gccgcgaaac ctacgaacga aataatagcc
     8041 cggccaaatt cataggggaa aatataacag agcttcccct aaattacttt acaaaccatt
     8101 ttggagtcca gccgtgggat gaatcactta ttcttgtcgg ttgtagcccg tgtcaatatt
     8161 acagtattat caatacggat aaaaacaaag cattgttgtc gcgtaatctt ttaatggatt
     8221 ttctaggttt cgtccgtcaa tacatgccag gctacgtgtt ggtagaaaac gtacctggaa
     8281 ttctcaccaa aaaagatacc gtatggcatg acttcaaatt tgctatgcaa aaattgggat
     8341 actatttcga ttaccatata ctggacctga gttattacgg agttcctcaa tcaagaaaac
     8401 gattttcatt tatagcttct cgtctcgatc ataatgttac tctcccgcct cccgataacc
     8461 gacaagccgt tctaaaggat tttatcgggg aaaaaaatgg gtttccaacc gtcccagccg
     8521 gatatagaga taatagcgat ttcaatcaca cggttgcggc tctctccgac gtaactttgg
     8581 agagactacg ccacactcgc catgacggag gaagccgttc cgattgggca aatattcctc
     8641 atttgcaatt gaaatgtttt gtaggaagag acgattgttt tatagacaca ttcgggcgta
     8701 tgagatggga caagccggcc tctaccataa ctactagatt cacaagtgta tcaaacggta
     8761 gatttgctca tcctgacgaa gatagaggaa tttctcttag agagggagcc acgttacaaa
     8821 cttttccaaa aaattacgtt tttgttactt cttccatgac agaaacggca aaaatgatag
     8881 ggaatgcagt tcccccagaa tatgctcgac gattaggtga aacaataaaa acactggaaa
     8941 acaatggcac aatttaaaac tcgagccaga gctctcgact tattgggtcg gcaacaaatt
     9001 gccggtatcc ctacagccat taacgagctt ataaaaaatg cacatgatgc gtattcggat
     9061 aagttcgata tcgattttct ccgatataat aatttactgg tattacgcga tgacggtctc
     9121 ggcatgacca aagaagaatt cgaaactcgt tggcttacgc tcggaaccga aagtaaatta
     9181 gcaaataaaa agaccagttt acctcctatt gacatttcaa agccaagacg tcctatcatg
     9241 ggagaaaaag gtataggaag attggctatc gcatcaatag gaagtcaggt cttaattgta
     9301 tcgaaagcaa aattaagatc taaagaatat gatattgttg tggctttcat taattgggag
     9361 atattcgagt tgccggggat taacttggag gatattgtta ttccggtaag agaatactct
     9421 catataccga atgctaccga tattgacagt attaaaaatg aagtaataca atcccttgat
     9481 aaattaaacc aaacaggact tattgatgac aaggattttg aaaaaataaa aagttctatc
     9541 acatcttttc aagttgatcc ataccaactt tccttacaac tacaacaagg atttgaatta
     9601 accaatggct gtggtggaac tcaatttttc atctccccgg tttacgatac gattatttcc
     9661 gatatagagg gagacggcaa ttcggacgaa gctacaaaaa tcgaaaaaat gttgatggga
     9721 tttcacaata caatgactcc cgatcatcct attcctgtcg ttgacatttc atttagagac
     9781 tataggacaa acgacggaag ttttgtcagc attatagata aagaacattt ttttacaacg
     9841 gaagaatttg aattggcaga tcaccatttt caagggcaat ttgatgaatt cggacaattc
     9901 aagggattag taaaaatata tggagaaaaa acatacgatc acattgtaaa ttggcgagat
     9961 aactcttata gagaaaccga atgcggtccg tttaaaatca acttggcgta tttacaagga
    10021 gaattgaaaa gttctcgtgt agatgtcgaa aattatgcca gaattaaagc caaaggagat
    10081 aaattcggag gtttatacat atatagagat aatattcgag ttctaccgta tggtgactcc
    10141 gattatgatt ttctagatat tgaaaaaaat cgttcgaaac gagcatcaac atatttcttt
    10201 tcttatcgac gaatgttcgg tgctattgaa atagcagacc gagaacacag cggtttggtg
    10261 gagaaagccg gacgcgaagg ttttattgag aataaagcgt atcgccaact tcaagcgatt
    10321 ttaaagaatt tctttgtaca gcttgctgcc gatttcttta gtgaaaaaaa taaaacggca
    10381 caatcggaat ttttcaacca gaagaaagac gaattcaatg cgtaccataa cgcacttgaa
    10441 cgtcgggaca aattggctaa atcgaaaaag gaaagattcg ctcgtgagct cgatatattt
    10501 tttgccagtt taacgaaaca taaattcgaa aacgaactgg aaggattact tacgaatttt
    10561 aggaacgatt tacattcggt tttatacatt aacgatgcgg atgaagcaag ccagaagata
    10621 atagatttgg aatctgccat gagacaaaaa gtttccgatt acagaaaaag gatatcggta
    10681 acaagtccta aaggatttgc aatgtcaaaa tcaatgagaa cggattttga tacgtatctt
    10741 aatgaattta aaattctgga acaaacgata tttaaaaata tcaatgaaaa tatcgatcag
    10801 ctaatagatg attatacggc acagttgaat ctggaaataa gtaagcgaaa gcggttggag
    10861 caagcggtgg aactgatttc agccgaagct ctgtcactta ataagaagaa gaaaagcgaa
    10921 accaatgatg ttgtttccga tgtttctcgc aaaattaagg atcttacgaa tgaactgata
    10981 atcgatttgg ataatcaaat acgaagcgta aaggatcaat ttaaattatt ggcaaccgat
    11041 aaagccgata actttgattt ggtaacggaa cgcaagagaa tggaagcaga aatagaatcc
    11101 ataagtagtc gaaatacgga tgttatggaa cgcataattc gacaatttga aagtttctat
    11161 gtcgaaaaaa acgaagacgg tcaaataatt acaaatgacc aaatagcgga agccacttcg
    11221 gaagagttgg aggagttgcg ggaacgattg caatcggacg ttgaattaag ccaattagga
    11281 ttggctgtcg gaatattgca ccatgaattc aacggtacgg ttaattcgat tcgacatagt
    11341 ctgaaagatt taaaagcatg gtcggatgta gacatcaaat tggaagggat atacaaaaat
    11401 atcaaggtaa atttcgaaca tttggacggt tatttaaatt tgtttacccc gttaaaccga
    11461 cgattgaatc gacgtagaga agacatatcc ttattggata ttaaaacatt tttaatagat
    11521 ttgttcaaat cccgtcttga acgacacaat atagctttta aacatactaa cgggtttgca
    11581 ggtcgaaaaa tacacggatt tcgttctaca ttctatcccg tatttgttaa tatcattgat
    11641 aatgccattt attggttaaa acaaagtgat gttcctgaaa aagttattcg gctgcatgcg
    11701 gatgatacgg gaatatacat ttccaataac ggtattgaaa taaaacccca agataaggaa
    11761 cgaatctttg aattaagatt ttccagaaag cccaatggaa gaggactggg gttgagtatc
    11821 agtaaagagg ttttgaatac agagaactat gatatattta tagcacaacc taaagaaggt
    11881 tctacagtta cttttaagat tcaaaaaatg caataaaaat atggctgaca aatttattga
    11941 gcaatcgaaa gagattgcaa acaactttat acaaaatat
//
LOCUS       DAWEMX010000075        11874 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2282_length_11874_cov_11.271766, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000075 DAWEMX010000000
VERSION     DAWEMX010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2282_length_11874_cov_11.271766"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3305..3314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2789 a         3514 c         3063 g         2498 t
ORIGIN      
        1 aatcagccgt ccaacgactt cggagacatg gacgacgaaa tcccgttcta aacgcaaata
       61 ccgccttttc cttttacagg ccgcctttcc tccgggtcag gcggtctttt tacatcatct
      121 tccccgaacg aacggctgcg ccgtaccacg aaaagaaaaa gtggtacagc cccattcccg
      181 gcaatggagg aaaagcatgg gaacaaccgg acaaaaggga aaacctcacc gttcatttca
      241 gcttggcaaa atcagtccgt cccggcatac tggcggcgta tgaaaatagc cgttattgga
      301 aaaggtggac gtgaacatgc actggtcaag gcgctcaagg aatctccctc cgctccggaa
      361 atgtactgtt tcccgggaag tgacgccatc aaccgtctgg ctacccccat cccggccagg
      421 gatctgccct cgctgatcga ctggatggtt tccaataaaa tggatctctg cgttgccggg
      481 gaggaaagct atctggtcaa ggatgaaggc ctggccaatg cctgctcccg tgcgggcatc
      541 ccctgctggg ggccggtaaa agaaagcgcc cagctggaag ccagcaagga atttgccaag
      601 gacttcctgc tccgccaccg catccctacc gggcaggcgc gcgtagcggc cacattggag
      661 gaagcccggc aattcatcgg aaacaactat cctaccgtgc tgaaatttga cggtctggcc
      721 gccggaaagg gcgtagccgt ctgcatgagc aaggaggaag cggactcttt cctgaaagaa
      781 gtatttacgg acagacgctt tggtgaaggg cggctgctgg tggaagaatt tcttaccgga
      841 ccggaagtct ccatcttcgg cgctctggtg gacgaccatt atctcattct ctgcccggca
      901 cgggactaca aacgcctcaa ggaaggcgat gcaggcccca acacgggcgg catgggagcg
      961 gtggcttccc gccggctggt ggagccggaa atgctggaac gcattgaaaa ggaaatcgtg
     1021 gctccgacgg tagccggact gaaaaaagac ggactgcctt accggggttt tctgtacttc
     1081 ggcctgatgc tgactccaaa cggcccaaaa gtaattgaat acaactgccg cttcggcgat
     1141 ccggaatgcc aggccgttat gcccctgctc tccggagact tggcttcctt ctgcctgcat
     1201 ggggccaagg gagaatttgc gccggaagaa atttccttcc aaaacggctg gagcgtctgc
     1261 tgcgtgctgg cctccaaagg atatccggag acctcccatt ccggcgacgc tattcaggga
     1321 ctagacgaca tttcaaatgc ttccgtttac cacgccggca ccaaatggaa tacggataaa
     1381 aactgttatg aaaccaatgg cggccgcgtc ctggccgtcg tggcacaggg agataccctc
     1441 tccgaagcgc gccagcgtgc ccacaacgaa atcaaaaaga ttcaatttaa cggcatgcag
     1501 cgccggccgg acatcggctt tgccagcttc gtctgatttt tccgcgtttc cctccccatc
     1561 tgccctatga aaccggcggc ccttctcatc ctcctgtttc tttcctgccc tgcatgcctg
     1621 atggcggaca acgccgtact ggcggccagg gcgcgtgaac aggtaggagt aacggtttcc
     1681 tacaacggag gctatcaatc catcccctac cccaacggag acgttcccct cgaaacggga
     1741 gtatgcacgg atgtggtcat ccgcgccatg agggccttcg gcctggacct tcaaaaagcc
     1801 gtgcatgagg acatgaaggc ccacttttcc aaatacccta aaatatgggg attgaaaacg
     1861 acggaccgca gcattgacca ccgcagggtc cctaatcttc gtaccttctt tgccagaaaa
     1921 ggttggtccg ttcccattac ggaaaacgcg gcggactacg agcccggcga cctggttaca
     1981 tggaacctgt ccgatagtat tccccacatt ggcatcgttt ccgaccggaa aacggaggaa
     2041 ggcacgccgc tgatcattca taatgtaggc gccggaacgc aggaagaaga cttcctgttt
     2101 tcccacacca tcacgggcca ctaccgtccc gtgctggctg agaggaaaca gaaagcgcaa
     2161 ccctgattct tacccccgtc ttagcgtaac ctgccatacc gggtatttcc ggaggttctc
     2221 ccggagttgc atggatatcc ggggaatgtc gcgggacagg aagaaggcgg ccctttccct
     2281 ctcattgatt catccggtcc ggaatgccca tgaaccttct tgccaaaaga gggggcttcc
     2341 gtcatcatgt gcgcatggtt acacctgcat tctccgactg tatcaacaag ctgggcgtcg
     2401 atgaaaaaga cgtcattccc ttcggtcgca acaaggccaa aatttccctg gacgtattgg
     2461 acaagccggc agcccccggc aaactcatcc tggtatccgc catcactccc accccatccg
     2521 gagaaggcaa aaccaccgtt tccatcggct tggcgcaggg attgcaggcc atcggcaaaa
     2581 aagtctgcct ggcgctccgg caaccttcca tgggaccggt gttcggccgc aagggcggag
     2641 ctaccggagg cggcaaaagc tccctgacgc cggcagagga aatcaacatg catttcacag
     2701 gggacttcca cgccattaca tccgcccaca acctcatcag cgccatcatt gataacgcca
     2761 tgttcttcca cacgctgaac attgacgaac gcaaagtcat atggaagcgg gtcatggaca
     2821 tgaacgaccg ttccctgcgt tccatcatcg tggggctcaa caagcagggg ttcccccgag
     2881 aaacaggctt cgatatcacc ccggcttcgg aaatcatggc gtgcctgtgc cttgccactt
     2941 cctacaagga catggaagaa cgcatcaacc gcatcgtgat cggattcacg acggatgaca
     3001 agcccgtatt cgcaagggaa ctgggcatta ccggttccgt catggccctg ctgaaagatg
     3061 ccctgatgcc caatctggtc caatccgtgg aaggagtgcc ctgtttcctg cacggcggcc
     3121 cgtttgccaa cattgcccac ggctgcaact ccgtgctggc caccaggatg gccctgcatt
     3181 ttggggacta tgccgtcacg gaagccggat tcgcgtttga cctgggtgca gagaaattcc
     3241 tggatatcaa atgccgccag tccggactgg atccggcggc cattgtcatc gtagctacgg
     3301 cacgnnnnnn nnnnatctga aaaacacgga cgtggatgcg ctgaaaaaag gccttgccaa
     3361 cctggatgcg catctggacg ctgccgccca ctacaaacgc cccgttgtgg tcgccgtcaa
     3421 caaattcttt gacgactccc gggaagaact ggacgctatc gtgaaacact gcgcggaacg
     3481 cggtattccc tgcgccattg cggatatctt ctcccaggga ggagaaggag gcaaagacct
     3541 ggcccaaatg gttgtggaag ctgcggacag gagttccgcc cctttcaagc ccctctatga
     3601 atccgccctg ccggtggaag aaaaactcaa catcattgcc cgcaacattt acggggcgga
     3661 cggtgtggaa ttgacagccg ccgccaaaaa gaagctggcc cagtttgaag ccagccgcct
     3721 gacggacctt cccatctgca tggcaaaaac ccagaactcc ctttccgaca acggacgcct
     3781 ccgaggacgc cccaccggct tcaccatcac cgtgcgcgac tttgaaatcg ccaacggggc
     3841 cggcttccta gtggccctct gcggggaaat catgcgcatg cccgcccttc ccgtctcacc
     3901 gaacgccatg cacatctacc tggataacaa gggcaacgtc caggggctct gaaaaatatc
     3961 accaacccgc cgcacgccat gaaaatcgcc attctgggag ccggagccct gggttgctat
     4021 tacggagcca gacttcagaa atcggggcag gacgtatcct tcatcgtacg ttcggaatac
     4081 ggctatctga aagaacacgg ccttcaggtg aaaagcctgc atgggaatat ctccctgccc
     4141 cgcatcaacg tttaccggga cacggaggaa gtcggcccgg tagacctcgt tgtcgttgca
     4201 tggaaaagca cggcaaacgc cggattttcc agagcccttc ccccgttgat ggggccggac
     4261 acaactgtgg ttacactcca gaacggcatg ggaaatgcgg aggaaattgc ccgcatcatc
     4321 ccggcggagc gtatctatgt gggcctgtgc ttcatctgcg ctatgcggga aaaaccgggc
     4381 catgtcaacc atctggaagg gggcaacatc cagttcgccc ccttcatccc cacgccggaa
     4441 gggactgaaa aagccaagaa actctccgag ctgtttgcca atgccggcat caaaacgcgc
     4501 gcctttgacg ccgcagagca aatccagtgg tacaaactcg tctggaacat cccgtttaac
     4561 gggctttgcc tggctctggg cggcatcagc attgcggaac tttaccaaaa tccggaaaac
     4621 gtcgtacggg cgcgccgcat catggaagaa gtagtacagg cagccagggc ccgcggctac
     4681 gccctgccgg aagatttggt tgaattccac ctttcccgta cggaaaccat gggggctttc
     4741 attccgtcca gcgccgtgga ctacaacgaa ggccgccccg tcgaatacac ggctatctgg
     4801 ggagaccctc tctccaaggc ccggcaagcc ggagcctccg tgccggaatg ggaacttctg
     4861 gacaaggcca tccgcaagcg cctaaacatg aattaactcg aaccaattgc gaatcatgct
     4921 gaagaccatc gtcattttca tcattgccct gctgggcttc ggcatgattc gcctgccttt
     4981 gcagaatcat attctggaac aggagaaggc ggaggggctg ctggataatc cggtagagct
     5041 ttcctcttcc gattatctgg aacaacaact tgccatggtc tccctgggag ggctccgctc
     5101 cctggtggcg gccgtgctga gcatggaagc tttcgactgt ttcctgattt ccgactgggc
     5161 caacctggaa cgccgctaca accaggttac ctctctcgct cctcactccg atttttactg
     5221 ggacaacgga tcgtggcatt tgggcaataa cgcttcctcc agttatctgg acaataaacg
     5281 tctttctccc atggaacgcc gggaaggctt ccgcaaatac attcaaaagg ggcgccgctt
     5341 cctggaaaaa ggcatcaaca ccaatcctga cagctggtac cttcaatccc tgctgggcaa
     5401 catgtatagc gatacctacc ggcaaccgga ctttgaaaag gcggcggaag cctaccgcaa
     5461 ggcacgggat ttgggagcgc ctccccttac ggcccgaaaa gaattttatg cgctcgtccg
     5521 tgtgccttcc aaatccagag aagctctgga actgggccgg gagttattca aggatccccg
     5581 caacaggaca cccagcctgg tgagtaatat ttttgttctg gaaaacaggc tgaacatacc
     5641 ggaaaaggaa cgcatcccct tccgggaact gtttcccacg gatgagatcg cccgtgacct
     5701 gatgaccagc catctggcca attccctgaa atatccggta gacgggctga gagaagccct
     5761 ggaatctttg cctccggcaa aagacgagta aatcaaacag acggaaataa ggggtcatgg
     5821 gaaggccggc accctgtgcc ccaaccatga tttcctgtga gcaaacgcgc ttggcaaaac
     5881 ctccccaaag tgctatgctc tttgcttaca ataccatgag tagaatagcc ctgattagtg
     5941 acatccatgc caatctgccc gctctggaag cggtgatgaa ggatatagaa cagcttcaat
     6001 gcaatgccgt ttactgcctg ggcgatgttg tgggctataa cgccaatccg tccgaatgcc
     6061 ttgaattcat ccgcagccgc cagattccca ccgtgcgcgg caaccatgat gaggaagcca
     6121 ttggagaaga caatccggca gggatgaacc cagtagctta caatgccctg atgtggacgc
     6181 ggcagcaact ttccgaagaa cagaaaaagt ggcttcgccg cgctcctttc cagcgcattc
     6241 tccccaatga aatcgttctg gtgcacgcta cacaggacaa gcccaactcc tgggcctacg
     6301 tcacgaatgt ggatacggcc acccacagca ttaatatgct gcgtgacaat caattcctgt
     6361 gctttaacgg acacactcac gtcccccgta cattcatccg ccatgaagga agcatccatg
     6421 aatatgaatc cggggatatc cagctactca aaaccaacaa atatctgatt aatgtgggct
     6481 ccgtaggcca gcccagagac ggcgatcccc gcgccgccta cggcgtgctt gacgaagaca
     6541 gcatggtgta ctaccagcgc cgcgtggaat acgatgtagc ggaggcccaa agacgcattc
     6601 tggccgccgg tcttccggac atgctggcgc gccggctggg agaaggaatg taaattccgc
     6661 acctcccgaa aggcctgcca aaagaaagga aacctgccgt attgcattgt ttccctttca
     6721 tcttaagaag aaccggacag cgccttatgt tagcaaaggc ttacaaattt gcattgctac
     6781 ttgcctcacc ttccacaaat cggtagagtt cagcgcgacg aatgaagacc tatatcatca
     6841 gacgactcct tctcatgccc ttgacgctcc tgggggttac gtttctcgtc ttttgcataa
     6901 cccgttttgt tccgggaggt cccattgagc agatgatgca gcagcaaagc atgagcgccc
     6961 tgtccggaca gaaagccgga tcccagcgcg gcgacaataa cctgagtgaa gcggatatgg
     7021 agcggctgga agagcagtac agcctgcagg agcccatcat cacggcttat ctacaatggc
     7081 tgggcgtgct gcccaaaaag attttcattt cccgtgagga attcggggaa atcggtggag
     7141 cgggagaggg agacaaggaa gatgaatatt ccggcatttc cgatacgcat acacggatca
     7201 atctgaatga aacaggagaa caggtcgtcg tagttcgcca ggacaaggat tccggcagcg
     7261 tgaaagatgc tttcttttcc ggcacgccgt cccggaaagc cgcttcagaa ggctggaccg
     7321 tggatataga atcccccatg gaccgcgcgg aacgctgggc gcgccgcatg aggcagacgg
     7381 ataatacgga ggccgtccgc gacaaggctc ggggctatgc ctggcgtgcc gtaatgtaca
     7441 agaccagctt tgacgggctg ctccagggaa ctatgggcaa ttcgtttaag tacaatgaac
     7501 ccgtatggga catgatcaag gagcgcattc ccgtttccct gtacttcggc atcctgagcg
     7561 ccattattac ctattccgtc tgcattcccc ttggggtagt aaaggcaatc cgccataaaa
     7621 gcctggtgga caacatctct tccgtcctga ttttcctggg gtactccgta cctgggttcg
     7681 ccctgggcgc cgtactggtg gtgtatctgg gcgcgcggct ggaatggttc cctctctgcg
     7741 ggcttacgtc tccggacttt gcggacatgg ggttctggca acaggccgga gacctgctgc
     7801 accatacggt gctccccctg atttgttatg tagtaagttc ctttgccatc accaccatga
     7861 tgatgaaaaa caacctgatg gacaatcttt ccgcggacta catcaggaca gccatggcca
     7921 agggcgtgag tttcagaggg gccgtcatca agcatgcctt ccgcaactcc tttataccca
     7981 ttgcctccac tctgggcagc ctcatcagcc tgattgtggc cggctccatg ctggtggaaa
     8041 aagttttcga tatccagggg ttcgggatgc tgagttacca ggctctgatg gacaaggatt
     8101 acgccctgat catgggaacg ctgctgctta cctccttcct gatggtactg ggcaaccttc
     8161 tctccgatat catcgtggcc gctgtggatc cgcgcatcaa atttgaataa tacacatggt
     8221 tagaaaactg gcataccttt tggtcattct ggcggttctt actgccgtgg gagagctgtg
     8281 cgggctgctc ctgcccacgt taagctggcc cttcaccgtc tcacgggaaa tgagcatgct
     8341 caacatcgtc tgtacggatc agaacaacgg cggccttctg acacctcagg ggaaattcct
     8401 gtggttcggc tgggtttgcg tgctggtcat gggaggcctc gggttctggc tcatgctgaa
     8461 aggcccccgg aggttccatc caacgccgat caccaggcgc cgcatccagc gttttaaaag
     8521 catctcccgc ggctacgtat ccctcctcat tttgcttgta ttgacgctat tggcctgtat
     8581 ggaccagtgc ctggtgggca agcgggccct ccttgtcgtt caggacggat cctggtactt
     8641 tccggccatg atgcgcaaag tatacaaggg ttctacattt ggccagaccg gagattttgc
     8701 ggatgcggaa gccaactacc gcgaactgaa aaaacaggca ggccagccgg gaaaaccctc
     8761 ccttgtcatc atgcctctgg ttccatacga tcccacggga gattccacca accccggttc
     8821 ggaagctttg atggtgaatg aagacggcct cgtctgcgag gccggcggca agccgtattc
     8881 cggtttggcc tcccgtttgc ataaggatga agaagccctt ccccacatca gctacaaatt
     8941 ccgcaaggga aaaaaagtgg accgggccac cggctggctg gaagacagaa cggaggtgta
     9001 cagcgccacg tatgaaaaca acaacattgt ggcggaacat tacagcggcc ccggcaccaa
     9061 agaggaattc ctgaaacaga cggatgaaca taaaatcagc cgtatcttct accacccctc
     9121 cccgcccctc aagggaggcc acctgcttgg caccaatacc cagggggcgg acattctggc
     9181 ttatctgtac ggcggcctcc aggtaaatat gaaagccgca ctcttctacc tgcctatcgt
     9241 atatttcatc ggcatcacct ttggaatgat gatgggatac ttcggcggca tgtttgacct
     9301 cggcatgcag cgcctcattg aaatcttctc ccaggttccc ttccttttca tcatcatgat
     9361 tatttcagat atggttcccc ttcatatgaa gggcatgttc ctgattatca gccttctaat
     9421 catgttcggg tggatgtcca tgacctacca gctcagaacc tccaccatga aggaaaaagc
     9481 gcgcgactac gtggcggccg cccgcgtgct tggggcttcc accagccgta ttctcttcgt
     9541 tcatatcctg cccaacctgg tcgccattct ggtcactctg gttccgttca gcgtttccgc
     9601 cctcattctg gctctggctt ccctggacta tctgggcttc ggccttcctg acacatacgc
     9661 cagctggggg cgccttctca atgacggact ggcggatctt tccgccagct gggtggttac
     9721 ctccgccttt tccgccctgg tgattaccct cctgctcgtc acctttatcg gggaggctgt
     9781 ccgcgaagcc tttgacccta aaaaattcac cacttataaa taaaaagact cccgctttgc
     9841 catttcttat gcttaagctg tccaccctcc tccgttccac cctttcttcc gtgatgctgt
     9901 cccttctgct gacagccggg tccggttgca aggattcctc ccaatcccag ggggtaggct
     9961 ggcagccgaa cgtacccttc ggcacgctcc ctccgggatt tgattccttc ccggaacgat
    10021 ggaacaaaca gatcaatgac cgcctggctc gtgaggaagc tgccaagcaa aaggaaatac
    10081 gggagctccg ggacaagttt tttaaagagg aagattctaa agtcagggaa aaactgcaaa
    10141 gcaaactcat tgcggatgaa gccgccctgt ccgtcattca ccgcaggcag acggaaggcg
    10201 actacatcaa attcaagacc ccggcggaca tcccccaaga cctgaagtgg gaggatggac
    10261 tggacaatcc ggagataggg gacccgaatg ccaaaaaagg aggcgtgctg cgccagtggg
    10321 cccccggctc ctatcccgac acattccggc ccaacggccc gaacagcaac agcggtttcc
    10381 gcggtcccct gtatgatgaa atcatcatcg gcctcgtttc catccatccg gttacgggaa
    10441 aaatcattcc aggaatcgct cataaatggg cggaatcccc agacaggcgc actgtttact
    10501 tcgaactgga tccggatgcc cgctatacgg acggagccaa ggtaaaggcc atcgacctcc
    10561 tggtgaacat gtatatccgc acttcggaat acagccggga cgtattctac aacaactttt
    10621 tttaccagaa cgcttccaac atcaccatct acgacgacag ccgcttttcc atcacactcc
    10681 ccttcgccaa acctctgctc ccgtactatt gcacgctgtt cattccgtcc ccgccgcatt
    10741 tttattgcga gttcggcccc aactacgtgg aacgctacca gtggcgcata ccgcccacta
    10801 cgggcgctta cgttgtcaag ccggacggta tcatccgcgg ccgccaggta acgctccagc
    10861 gcgtgccgga ctggtgggcc aaggacaaaa aattcacgaa atacatgtat aatgtggatc
    10921 aaatcgtgta taatttcatt gcggaaccat ccaaggccat cgaactcttc cgcattggag
    10981 aactggacgt tttgaacatc accaaaccgg aactatggca cgaacgcatg gaaataccgg
    11041 aggttcacaa cggctacatc aaccgcagca cattttacac catttacccc cgccctcctt
    11101 acggagtttt tctgaatacc tccaaagccc ccttcaatga cctcaatgtt cgccgggggt
    11161 tccagcacgc acttaatatc cagaatatca ttgacattac cttccgcgga gactaccagc
    11221 ggctcaattc ctacaattca ggtttcggca aattcaccaa tccttatatc aaggcgcgcc
    11281 cctattcccc ggaacaggcc cgagcttatt tcgccagggc gggctacacc atcccctgcc
    11341 cggacggtat cctccgcaaa ccggacggca cccggctcac cgcagccatc accttcccca
    11401 attcatctcc ttccctggct tccactctgg gcaagctgaa ggaagacgcg cgcaagtgcg
    11461 gcctggaaat ccagcttgac cctctggact ccaccgtagc tttccgcaaa atcatggaaa
    11521 aacgcgctca ggcctccttc atggcctggg gattcactcc cccccatccg atgaacgagc
    11581 aggggttcca ctcccggtac gcctatgatg agcgtggagg ccttattacc tataccaata
    11641 acatctgcgc ttacgcggac aaagaaatgg acaagctgct ggacgcagaa acaaacgcag
    11701 ccacggaaga cgaactccaa aaggccacgt ggaaagtgca gcagaaaatt gatgacgaag
    11761 ccctgtgggt tccctgctgg accacggaat tcatcagact gggttactgg cgctgggtca
    11821 aatggccgaa cagtgcaact acgcaattct gccatcccgt cgtatttgat ccca
//
LOCUS       DAWEMX010000076        11851 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2289_length_11851_cov_12.143269, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000076 DAWEMX010000000
VERSION     DAWEMX010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11851)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2289_length_11851_cov_12.143269"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2036..2045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6085..6094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6625..6634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2178 a         3284 c         3499 g         2860 t
ORIGIN      
        1 aaggggctaa ctcgggttga cggcggttgc ggtatctgcc gccggggcat cggctgcggg
       61 agcaggggag gaagctgcgg ttttcccttc catgggtttg cccagaaaag cgggcaattc
      121 cagccctacg cttttgccga gttcatgaag gggcagcgta tcctttacca gattctggac
      181 gaatcctccc acagaggagt tccctccatt gcccatgacg actaccttgt cgaatttcaa
      241 ccccttaatg gcgccggact ggagctccac aatcttggta agctgttccg tcaccagcag
      301 gttggaagcg gcctcggaag aacctgccgc ctggacgatc tgctgaaaac cgcgggcctt
      361 gccttccagc acggcttcaa tcgcggccgc ttcaccgcgt cccaccattt ccatgccttc
      421 cccttccgcc tttttcttca ggagggtagc ctgggcttcg ccttccgcca gttggcggat
      481 ggcttccgct tccgccctgc gctggatgag cagagcctgc gccttgcctt cctgttccag
      541 tttgagtact tccgccgtgg cctgggcctg cacttcgcgc tggctctttt caatgcgggc
      601 cgttaccagg atgttggctt cctgcgtggc gcgttcgcgt tccgcgcgct tcaattccgc
      661 ttctttttcc gcctggtagg cttcttccag ggttttagcc tcctgcacct tctgggccac
      721 ttccgccagc tttttggcct ccgcctgctt cacttgcagc ttggcggcgg attccgcaat
      781 tttaatctgg gcttcgttct ggccctcaat ggcgatggcg cgggcgttcg ccacctggat
      841 ggtctggtcc ttttccgctt ccgctttgcc gatttccccg cggcgcgttt cttccgccac
      901 cttgatggtg gcgtcattga tggcgcgcgc agcggcttcc ttgcccaggg cgttgatgta
      961 gccggaagcg tcctggatgt ccgtgatatt ggcgttgatg agatggaggc ccactttgtg
     1021 cagttccacg tccacgcctt ccgtgatgcc cttgatgagt ttttcgcggt cggaattgat
     1081 ttcctcaatg gtcatggagg caatcaccac gcgcatctgc cccatgatga tttccgcggc
     1141 caggttgcgg atttcctccc gggagcggcc cagcaggcgc gcagcggcgt tctgcatgat
     1201 ttcagggagg gtggagatgc ccacgatgaa ggaggagggc acatccacgc gtatgttctg
     1261 ggaggaaagg gcgccctgga ggggcacgtc aatattgata gggttcagat ccaggtagct
     1321 ataggactgg agaaccggaa gcacgaaggt ggagccgccg tggtaacatt tggccggctg
     1381 gcctgtgcct actttcccga agacgatgag gattttgtcc ggagggcaca tgcggtagcg
     1441 gctgaacagc caggaagcgg tcagaatgat gaagagaacc agaatggcga tggggatgat
     1501 tttgtccata gtcggcttgg ggtatgatga ttggggtgtt tgttaatgaa gaggtttgac
     1561 ggtgacgatt cccgcagtga cggctaccac ttccaccggg gttccggagg gcaggggatg
     1621 ttccccttcc tggacggcag ggaggtacag cagctggctg ggatgggcga ttgtaacttg
     1681 tccgccgcgt tggaggtttc cgggaatgct gatgtaaacg gagccggtca ttcccttgag
     1741 ggtttcataa ttcagggtgc cgtctgattt aaggctcata atgaaacgca tggagatgcc
     1801 gataatcagg aacatcagca cgccggtgaa aaaagccgcc gccagggagg cggccatccc
     1861 ccagtccaga ccctgggcga gcacgccgcc ccagccgaag cccagaaaaa agccgatgcc
     1921 ggatttgatg gacagcaacc ctacgtccgc tccgccggaa tccgcatcaa agtccagatc
     1981 atgttccccc agcccgaaca gggacaggag aaacaggagc gctcccacgc ccgtcnnnnn
     2041 nnnnngatga taaagaaaat ggagtaatca gtcatagggt ggattgatta ccgggagtat
     2101 ccatgtttgg aggctgcttg tctatggaaa aagagcggcg gccggatatc cggccgccga
     2161 cggaagggga aaaatccctg caacttgttt ttcttccacg cttattccat ttccacgacg
     2221 gctttttctc cctttttgag ttccagttcc cggagcagca ggaagtcgtt ggtcagcagg
     2281 cctgcctttt caatgggagt ggcggttccg ccaatggtga cggtggcctt gccggcgttg
     2341 atccgattgg ggaaccagac tcccaggcga cctttggcgt ctttgttggc tgtgacggtg
     2401 aaccctttgg aggcatctgc cgggtcattc cagtcgatat gccacgggga cggagcccgg
     2461 gattcgtcca ggcgcgccgt ccaggcgggg tctccgtaaa aggccacggt atcccgatcg
     2521 tggataagcc ccagctggtc cttgcccatg ccgtatccgg cggagttcat gcccctggca
     2581 aagtccggat catccttgat tccattgata tccgcggagt tgaaatggac gtccatgagc
     2641 ttggggaagc gggtcatagt ctcgtccaga atgaactggt tgttcaaata ccaggcttcc
     2701 gccaggctgg aggcgtcatg attgctgaac agaaggccca gcgtccccca gccgcccttg
     2761 ccataccagg aagggacggt gtagcctacc agctggttga agccgtagcg gctcagggcg
     2821 gtgacagcca tggaattttt cgtcttcttg gcatctccga tcaagcagtt cccagccgcg
     2881 acccagacag ccggaaccgg cctggtttca atcaccggcg ctttaattct cttaaggaag
     2941 gagagaaggt cgtcttcctt tccgttgaag agcacgccgt gcaaaaaagt ggtgaattcc
     3001 ctgaattgtt ttttgtccag agcgtagaac cggttgttgc cggaaacgat gagtcccttg
     3061 ccgaaaggca tttccagatt aaactgggtt gcgtgggagg ccgttacgaa gagctgcggg
     3121 gaataacgtt tccagtattc cataagcttg ggcgttacgc ccagcgtggt ttcatccccc
     3181 ttgtcctgtt ctttcaggcc tttcgtgtag aaagccggcg ttaccttgcc cttggagcca
     3241 tgctgttcca ggtattggaa gggctgccag tccgtgatgc tcatgctgtc tttgaatcgg
     3301 gaggaatcca cgttggtagt tcccatggca cggctgatga cgagaggctt cgtttcagag
     3361 gctattctca tggcggcctg cggggtgtac cccgtgacaa tgccccagat acagtccccg
     3421 taagggtcgt cgtccagccg gcggctcagg cggtgcaggt cattcaccag cacccggtct
     3481 atttcctccg ggcgtgcgac gacggccatg aaacgtggag ccattttttt gagcgtgtcc
     3541 agcttggaga atacggaatc tttcacggtg acgatggagc ctccgtgttt ttcagccagt
     3601 ttgtccgcca cggccttcca ggccggcatt gaagctgttt cttcggaaat gatgatggca
     3661 tatccatttt tgccggagtc tttttttaca gtcttcacga cggtggcgcg aacgcaaaaa
     3721 gccgccgggg agacggcggc ctggggagag tcggctttgt ccgcctgctt ttcagcaggc
     3781 ggagtttccg cagccgggtg atccggcaga atgttcaggt tgagatgctc tcccttgccg
     3841 aagatgtctt tggcaagttt gttgacctct tccacggtga tggcttttac gtccggaatg
     3901 ctttccctct gctggttcag ccgttccggc ttggcctggg agtctttcag cagggatgtc
     3961 cagtaaccgt tgtcgcgctg ggcgcggtcc atggagttaa gaatgggatt gcgggcacgg
     4021 tccagttcct cctgggtgat atttcccttg ccgaggtcgt ccgcaattct ggcgatggcg
     4081 ctgcgcacgg cctccttatt gcgcatgacg ccggaactca tcgtgatgat atagccgtcg
     4141 tccggatagg tttcactgat attgaggccg gtggaagggg agtaggtttc ccccatgtct
     4201 tcgcgcaggc ccttgaatac gcggtcatag aagacggcct tgagcatgtt cagcctgcgg
     4261 gccagctttt tattttctcc gccgggcgtt ttccagaaaa ggcagaccat cgttttatca
     4321 atggaggagt cataggtcag gtccttggag aaattgaagt ccgccatggc cggatggcga
     4381 agcgtttcat ccagcgttgc cggagcttcc gcacgtttgg gaaccgctcc tacggtgcgt
     4441 tccagcaggg ggatgatgtc ttccgtcctg aagtctcccg taacggtcac ttccatgtag
     4501 ttgtttttca acggggcatc cacccagtcc cgcacgtcct tgacctggta ggaggtaagc
     4561 tgttcctggg tggggaaggt gaaccgggga ttgttcttgt aaaggatggc aggaacctgc
     4621 ttcttcatgg ctccctgcac ttcatggtcc atcttgttat aaatcatggg aatggcccgc
     4681 cggagcaggg taacgccgtc ctgtcggtaa ccggggtgca tcaggtaggc cgtctggagc
     4741 tggagttgcg tttccaggtc ttccctgttg gtgtttccgg aaagcaggaa gaagcggtcc
     4801 gtcatggaga agcctacccc taccttcttg cctgccatga tggcggccag ttcgtcattg
     4861 gagtggtctt tcaaaccgcc gccgttcatg acggcattcg caaaaagttc cagcccggag
     4921 gctttttccg gcctgctcag ttccccgccg tccacggcaa aggtgatgtt gatggagtcc
     4981 ttgtcaaatt ccgtgggctt caggttgacg cgcacgccgt tggacagggt gagctgggtg
     5041 actcccaggt cggtggtttc cgtacgggcc gtcaccttgc cgggatctcc gaacttatag
     5101 gaaaaatcct tgcggctgtc cgcctggtag ggctgtactt tagcggtttg ggattcccgg
     5161 tacgctttca tgatttcggc gcttccctgg gggttttcct tattggaagt gacgatcacg
     5221 cggggaaagg ctccggtcca ggcttccttc agggctgcct ggcattgttc gggcgtcagg
     5281 ttttccacga cttccctgga gatggcccag tcttcctgcg gcgtggtgaa gaccttgtcc
     5341 cgtgcggcgc tctgggcgat ggcggaagcc aggtcttccg acttggcggt ggcccaggac
     5401 ttgatggcgt tttccgcggc ggcggtaatg ttgctgcggg cctccgccag ttcttccttg
     5461 ttgaagccga attcgatggc ccggcgcagt tcctgttcaa tggcggccag agccgatttc
     5521 caattcttgt agtccgcctg ggtctgaatg gaatccactt ccgccgcttc cactatgtcc
     5581 atgcggcctc cttccgcgga gataaagggg cagtccgcat tcttcgccat cttttccagc
     5641 cggcggttca gcatggcgta agccacgttc agggggatat ctttgttgcg gttggcgacg
     5701 gtatccggct ttttcacata gggccgggcg atgttgatgc tggcttccgt gctggtggcc
     5761 tctttgttgg taatccagtg ggcggtggtt tccgtagccg ttttaagcgt gccccggtcc
     5821 gtctggaagg aatagttgtc cttcttcatg gagccaaaat acttttccac ccaggctttt
     5881 ccctgttccg gtgtgatgtc cccggcgatg acgagctgca tttggctggg aacgtagtgg
     5941 gtccgataat aattgataaa tttttcccgc ggcgcggtgc ggattacttc cagcgtgccg
     6001 atgggatagc ggtcgggaat gcgggtgccg tccagcatga tggagaagac ttccttcatg
     6061 acccggtaac cggcggagtc gcgcnnnnnn nnnngagtcg cgcaccttgt attcgctggt
     6121 gatgatgccg cgttccgcgt caatggcgct ttcttccagc agggctccgt ccgcaaaatc
     6181 ccgcatgatg gtgaaagcca ggtccacggt ggattccttc atgctgggca cgtccatcat
     6241 gtacacggtt tcgtcaaagg cggtgtacgc atttgcgtcc cctcccaggc ccaggccttc
     6301 tttctgcatg gcgggaacca tttccccgcg cttgaaatgg gtgcttccgt tgaaaaccat
     6361 gtgttccaga aagtgggaaa cgccctggat gtcatccgtt tcattcaggg aacccgtgtt
     6421 gacgcgcagg cggatactga accggccctt gggttccgcg ttcgggcgga tgaagtaggt
     6481 caggccgttg gcgagcttcc cctggataag ctgggggtcc tgcttgggaa tgcctgccgc
     6541 cgtttgcatt gaggcagcct gaatcttgtt tcctgctgca gttccggccg gtgaatagga
     6601 cggaaatagg cacgcagcag ccagnnnnnn nnnncagcca gaatggaggt gatggtaaat
     6661 gccttcatgg gaaaatactg cctttttgtt tcacacgatt ttccgggcaa gtcaagcggc
     6721 cttctctgca taaaagaggt gaatttccgg aaaacctcag gaaacgtgaa ccatgtcaaa
     6781 gggatgaaga aggcatccat tcatcccgtg ggcgcggttc tgaaattccg cagatgcggt
     6841 ctacacaacg ttcccatgcc tcccggccct ggtaaatttc cacctcaatg cgcaggaaat
     6901 agatagggac ctccggtttt tcctccggtt tttccagccg cacggcatcc ttctccgtag
     6961 ttacgatcat gtccatggcc cggtctgcgc agcggtcgta aaattcctgc aattccgttt
     7021 gttcaaacca gtggtggtcc ggaaacctgc ggcagatttc cacgtgggcg cccagggagc
     7081 gcagggagtt ttcaaaactt tccggacgtg cgatgccgct gaggcacgcc acccatttcc
     7141 cccggagggc ctccagagga aggcgttccc ccgtgaacac attttccaga taccgggggc
     7201 cgtggtcgct cacgatgatg tccgccacgg gattgtactt cctgatggcg gagatcagct
     7261 catcctgtgg ctttccgccg catttggtca gaatgatgta actggcgcgc gccagactgc
     7321 tcctgggttc ccgcagggtg ccgcgcggga gcatggctcc ggtgccaaag ggcgcgccgc
     7381 aatccaccag cacgatatcc agttcatgcg ccagtttcaa atattgcatg ccgtcgtcca
     7441 gcaacagggt gtcgcacccc aggtgctcaa tggcgaagat gccggacttg atgcggtttt
     7501 tatccaccag caccgccacg ccgtccagat ttttggcgag catgaaaggt tcatctccgg
     7561 aatgcagggg gcccaggtag cgcgttttcc cgtcgctggc gattttggga agattctccg
     7621 gcagcctgcc gtccttgtcc ctccattcct gaggtttatc cagctctgcg ctcttgtagc
     7681 cgcgtgtcag gatggctact ttgcgcccgc gccgggtaag ggtgcgggcc agcagttcca
     7741 ccaccggggt ttttccggtt cctcccacgg tgatgttgcc cacgctgacc accagcattc
     7801 ccagcctggc ctgcctggcg atgctggaat gaaacagata cagccgtgcc agaaccacca
     7861 gcctgaacag ccaggaggcg ccgcgcagga tcatgcgcat catggcagcg cggaatccct
     7921 tggcacggcc gaagatgact tccgtccccc actcttcaaa ttcttccgat ctggatttca
     7981 tggcaagccc ggcggcgttc ctggaaactc cttcagtgga ggatgcggtg gcagctgtcg
     8041 cagtaaacgg tctcgtggcc gcccagcact ttcatgcggg cgttgtccgt gataaccatg
     8101 tggcagccgc cgcaatggcc tttttcatcc attggcacaa tgaccggaac ccccttgctt
     8161 ctggtcatcc gttcatattc ccccagggaa ttttccggaa cggcggcggc cagatccgcg
     8221 cgttccgcat tcaagcggtt caggagctcc ttgtccgtct ctgccgtccg gtcaaagcgc
     8281 gcgagcgttt cctccagttc acgctgggcg tcccgtgcac ggcggatttt ctgctccatg
     8341 tccgtttttg ctgtttccag gcgctccatc aattccagct ctgaggtttc cagagagtca
     8401 atagcggctt ccgttttttc cacctcctgg atgcacatct ggtattcctc gtttttccgg
     8461 gtgttggact gaagggtttt cattttgccg atgtaggcgc gtttggtttc aacggcggcc
     8521 tccacgtccc gtatgctttt ttcaatgcct gccacttcct gtttggcggc cagggccttc
     8581 tgcttgatgg cctccatctg cttcagcagg cgcgttctct gttccggcag ggaagccagc
     8641 tccttccgga gtttggagat ccgcacatcc ttttcctgaa ggatcagcaa ctggtctaaa
     8701 tcggcatgat tcatcgcctg ccaccctagc atgccctgcc ggggggggaa agattttttc
     8761 ttggctccgg ggcggcggtt ctgccgcctg ttgacggtgc ggggaagtct gagaaatatt
     8821 tttctgttct ctgctgagta tttcaagcag ggctttctgt tgtgccggcg tgcttccctt
     8881 cgaggtctgg ggcatggaaa attccgcgta ctgccgcgac agccggtctt ttttctcctg
     8941 gttccgccag gaaaggaagg cgcatatggc aagggcgcag acgcagaaga ctgccaggac
     9001 catgtatgaa tggcggttgg agggcatgga ggaagggaac tgccctgcat tatagaaaaa
     9061 actgtcttcc gctcaatgta aatagacagg caggggaaga cggcgtttgc gggggaagat
     9121 ttctgttcct gtctgccctg agcggagaac gcttccagaa agccgggaat gttcagggag
     9181 aaagcttgtg ttggccgtcc gggatgaatg ggcgatgatg ctgggtgaat ggggaaagag
     9241 ggggacaacg gactttccgg gccatggtgc ttccgtccgg atgtttttcc tttgcagaaa
     9301 ggaccgttta gaaacccagg gcttccttct gccagttcaa cagggtggaa acgccttttt
     9361 ccctgtcttc cgcaatagct tccagagagg cccgggtaat caggaaggag ccttccatgc
     9421 ccagctcttc agctttttca tccctgagtt tgcacaggcg ggccagattg tcctcaaatt
     9481 ggtcggaatg ttgccggcgc tgaatgcggg gccggcatgg gtagtcttct tcatccagca
     9541 ggtagaattt ctggagcgca ttcatgaacc ggctgcgcct gtgggcatga aaacggggcg
     9601 ggggcgccac ggtgcgctgt tcctggagag ccacgctcca ctggatgagg tcagcattgg
     9661 agcaaaccat gaacgcgggt ttgtcccacg ttttggcttc cgcatcacgc caggtccaca
     9721 tttcccggag ggcggccagc cccttcctgt tcaatttgcc gcagccctgg atgcgccagg
     9781 ggtcctgatg gcctgccaga tggcgttccc gggcgcgttc catggaatgt ctgcaaattt
     9841 cttcaaacca gcccatgcgt cctttttccc ggagggcggc cgtcagtttg tccgccatgt
     9901 ccagcatgta atttacgtcg ttcagggcgt aagtgaccat ggttggggaa agggggcgcc
     9961 gcgcccagtc cgccttttgg gaagatttgc tcagggtgat gccgtggaag tgttccacca
    10021 gggctgccag cccgaactgg cggaagccca gcaggcgcgc cgccgtctgc gtatcccaga
    10081 tcatggcggg cagcgtttcc caggcgtttt gaaagaggct catatcgtag tccgctccgt
    10141 gcatccagac ttccgtttct ttcagccagt tgtagaaagg ccccatgtct tcgatggaga
    10201 gagggtcgat caggcaggaa cccgtttcgt ccgcgtattg gatcaggcag attttttcct
    10261 gatagcggtg caggctgtct gcctccagat ccaggactac ccgtcccgcg ggctgtgcgg
    10321 cggcgcgttt gcgccattcc agtaattcct ctttctcgct aatcatctta ttgcaacgga
    10381 gcgggattga accatgtatc cggacgccgg gcaactggaa atgcaaaaaa agtgctgatg
    10441 gggcaatcat gtattgccta tttcccggaa atagggcacg aaggaaggcg tatggacgag
    10501 gaagccacag caaccggacg caaccacgga gaacagcctt tggatgaact gatgaagcgc
    10561 tggcatctga ccaatcacga cctggtggaa atttctccgg agcagcttac ccacaagcag
    10621 gtccaaaaag cccggcaggg gcgccagctt accctgaaaa tgatgcagaa ggtatgccgt
    10681 gccttgaatg tagccatctg ggaacggctc acccccatgc agaaagagca gtacttcgaa
    10741 tacatgcaca agcatgtgtt cagctatgcc aagggatatg atccggcctg gaaagacccg
    10801 aacatggaca tgatggcctg accccggatt ccgttaccgg gcggattttg cctgctgcca
    10861 gcgtgcctcc atggcctccg cagaagcttc ttccagggaa agaccgtctt tggcaagcag
    10921 ccgttccatt tcgttgaaac gccgctcaaa ttttctgttt gctccggcca tggccgtttc
    10981 cgggtcgatt ccttttttcc tgcacaggtt gactacggaa aacagaagat cccccaattc
    11041 ttccgcgacg cgggggtctt cctccggtgc ggaaagaatt tccccgcatt ccgcggtttc
    11101 ttccttcacc ttgtcaaggg cgccttgggc gtccgcccag tcaaatccta ctttggcggc
    11161 ttttttctgg agcttccatg cctggagcat gggcggcagc cccttgcctg ttccatgcag
    11221 gtatggggtt gtttccgtcc ccttttcccg gcgtttgatc ttgtcccact gagctaatac
    11281 ggcatccgtc gtgtccgcct tggactgggc aaatacgtgg ggatggcggc ggacgagttt
    11341 ttcgcttact tccgcagcca cgtcattgaa atcgaaacgc cctgtttcct gggcaatctc
    11401 cgcatgaaaa accacttgaa gcaaaacatc gcccaattct tccttcagct gcgtccagtc
    11461 attccgctga atggtgtcca cgacttcata ggcttcctca atcatattgg aaatcaggga
    11521 atggtgggtt tgctccgcgt cccaggggca gccgtggggg gcgcgcagac gtttcatgat
    11581 ggcgatgagg cgctgcattt gaagggcagg ctcgcggcat tcgatcattt cagtgtcgtt
    11641 catgacggag ccatactaat cgcgcaaggg cggatgacta gaaaaaattc ctctttaccc
    11701 gaaacgcttc ttccttacac tcgcctccat ggaagttcac acgtggaaag aacgcacgga
    11761 agagggaacc ctggtgtaca gggcatgcca ccatgccggc aaatggaagc tggagtgcgc
    11821 ccccaaggtg ggccgtgcgg aacgtgacga t
//
LOCUS       DAWEMX010000077        11801 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2305_length_11801_cov_12.700494, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000077 DAWEMX010000000
VERSION     DAWEMX010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_11801_cov_12.700494"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2699 a         3572 c         2958 g         2572 t
ORIGIN      
        1 ctgctgtacc tgcacagcaa gaacctgacg caccgggaca tcaagccggc gaacgtcatc
       61 tttgtcaacg gacgccccaa actggcggac ataggcctgg tggcccatct ggaccagcgc
      121 agctttgtag ggacggaggg attcatcccc cccgacggcc ccggcacccg gcgggcggac
      181 gtttacgcgc tggccaaggt tctgtatgaa atcagcacgg gaaaggaccg catggatttt
      241 cccgaactcc cggatgactt gccggaaggc accgtgcgca agaaatggca ggccttcaac
      301 accatcattt gccaggcggc ggaaccccgc attgaagaat gccccattga ttccgcagag
      361 gagctggcgg aaaaaataga cgtcctccgg ggatatgaag tgccttcccg tttccgtctt
      421 cagaagaaaa agaaacgccg caaactcaga cgggccctcc aacttctggc tgcagccgcc
      481 gccggagccc tgactgccta ctggggggct ttatggctca acaacagcca gcatccgccg
      541 gaggaacctg cctcggatat tcatccggat atccactcat ccgctccgga agaagaatcc
      601 cggaccggat acgtgctggt gaccagctcc ccggcgggag cgtccgtgta tgatgcggac
      661 ggcaactatc tggatgaaac tccctatggc cccattgaac tgccttccgg ctccccggta
      721 gcctatacca tcaagaaatc ggggtttgcg gacaaggaag aggcgggaac cgtcaaagga
      781 gggtccacac tggccctggg cggcgtgctg aaggagtacc atcctcctac ggacagccag
      841 ccctggaagg atacggaggg tgttacgtac ctgcccacag aaacgcgcca tgtagcgcaa
      901 ggccccctta ccgccgccct gttcaacaaa tttctgaggg aggaccgcca gaaagggaac
      961 ttccagatga aaagggaaca gacggaaccg gggcatccgg agaaggatgt ggccctgctc
     1021 acgcaggacg gcatcacggc ctacctggcg tggctgaaca agaaatgcga gcgggagggg
     1081 ctgctgggca aggaattttc catcaatgcg gatccgcttc cgcaggcttc cggcagcacg
     1141 gaaaaccaca atgcctacgt cctgaatgtc acccgtgtgt tccaggtgcc cattaccgtc
     1201 accaccaacc cgccgggagc cagcgttttt ttcaataaca ggctcatcgg gcgtaccccc
     1261 attgaagaat acgtcaacca ggttccctac gtcattgaaa tcaaactgcc ggggcacgcc
     1321 accatgcgtc gcagggggct ggatccgcag gacttatacc tttccctgca gttggaacct
     1381 gacaagtccg ttgtctttgg ctcgccctgg accaacagcc tgggcatcaa actggtccct
     1441 gtgggaggaa atgctctggt catgacccac gaagtgcgcg taaaggactt tcaggaattc
     1501 ctgaaagcta ccggaaggaa agctccggcc cggcccggct ttccccaggg agaagaccac
     1561 ccggtggtga atgtcagcag gcaggatgcg cgcgccttcg ccagatggct taccagcaaa
     1621 gagcgggcac tgggcctgat tgatcagcac gactcctacc gtcttcccaa ggatgaggaa
     1681 tggagttcct gggtgcgtct gacggatgaa cagggagcct ccccctatga aaagacgctg
     1741 cctcatgaaa actcccggga agccttcccg tgggggtatt catggccgcc gcctgataaa
     1801 acgggaaatt ttgcggacca gtccgccctg atttacctgc cgtcctcccg cgttatcgta
     1861 ggatataacg acggacagcc gtatacggcc cccgtaaaaa cctttccgcc caaccacctg
     1921 ggcctgtacg acctggaagg aaacgtcatg gaatgggtag acgattccta cggggggccg
     1981 gaatccctcc ccatccgcaa ctatggtgtc gcccgcggag gcagctacct gtccttccgc
     2041 cccaaacagc tcaccacttc catccgcact cccctgccgg aaaatacgag ggataacgcg
     2101 ctgggcttcc gtcttatcct gtcttccgaa cgccccgcca ttccaacagc tccctgacgc
     2161 cgtatctgcg gcagcggttt tccccgctct ttccccctct gaaacacacc gcccccccaa
     2221 gggggaaagc cctgaggctt gagagagctt ccggctctcc gggaaaatga ttgaaagggc
     2281 ctcccgttat gctaccattt ttccgcatcc ttttcctgaa tgaaaatcat cgccatcgcc
     2341 aaccagaagg ggggagtggg aaaaaccact acggccatca acatggctgc cgccctggct
     2401 cagctgaaaa agaaaatcct tgtcattgac ctggacccgc aggccaatgc caccagcggc
     2461 atgggcattg acggaaaaga catgcccagc ctgtatccgg ccctgctcgg ccagcttccg
     2521 gcggaagagt gcatcctgcc ttccggcagg aaacggctgg atatcatccc ctccaccatg
     2581 gacctggccg gcgtggaaat agaattggcc cgttccggca accatctgac ccgcctccgg
     2641 gaaacgctcg ctcccctgcg ggagtcagac acctatgatt actgcatcat ggacacgcca
     2701 ccttccctgg gcgttttaat gacctccgcc ctggccgcct gcgacgaagt gctgactccc
     2761 ctccaatgcg aatggttcgg tttggaaggg ctggccaaga ttctgcacgt gacggcgcaa
     2821 atctgcgcca gcggcgccaa tccccgcatt cggcatgaag gagtactgat gaccatgtat
     2881 gacggccgca ccaatctttc ccgccaggtc atcgaccagg tggaacggca tctgccccag
     2941 atgctgtaca aaacggtcat cccccgttcc atccggctgg gggaggcccc cagtttcggc
     3001 cacaccattt ttgaacacga tccttccgga gctgcggcgg acgcctacct gaaagcagcc
     3061 cgggaattca tcaaacgcca taaaccctga acatttggag aaacaagctt cccggaatgc
     3121 cgcaaacctc tgcgggagga acaggcagaa agggcaccgc agaacacttc ctccttcatt
     3181 ttgccttccg ggtttgttgc gttcatttaa cgtcaggaaa acctattcca taccggcctc
     3241 cccggatacg gaggaaaaca ggcattcccc ttgacttgac ttccgcggca tataagaatg
     3301 ctccagctcc gtttcaggaa cttttcccct catgccagca gaagaaggcc agccagacga
     3361 ccagccccgg gtgacagggc aagtcccgcc tcccgctccg acgacaggcc tggaacagat
     3421 tctgcccgga cgtccgcccc tgccccatgc ggaagaaaaa ggggcctttt tcgtttacca
     3481 gcgggagctg gctcccggca tgcgcctgga acaatatgaa atcatccgcg tcctgggcag
     3541 cggaggtttt ggcatcacat acctggcgaa ggatctgttc ctgaaccgca acgtggttat
     3601 caaggaaaac ttcccttcaa gatactccta ccgggaccct ctgaccgggc atatccggcc
     3661 caataatgaa cacgatctgg aaaattacac ctgggctctg aaaagttttc tgagcgaggc
     3721 tcagacgctg gcggagctca acaaccccgg catcgtcaga atcctttccg tctttgaagc
     3781 caacggcacg gcctacttcg ccatggagca tattaccggc ctttccctgg attatctggg
     3841 ggaaaaactt cacagcaccg gacaccggta tacggaagac gaactcaagg ggctgctgac
     3901 gcgtctgctc cggattctgg actatctcca ttcccggcac atttaccacc gggatatcaa
     3961 acccggcaac atcctgctga cggaggaagg cacgcccgtg ctgatcgact tcggagcggc
     4021 acggcatgcc ctgaaacttc atgccgccac cgtcctgacc acccaggggt attcctcccc
     4081 ggaacaggcg ctgggcaaaa cgaatatcgg cccgtggagc gatctctatt ccgtaggggc
     4141 cactttttac gccctgctta caggaaatcc tccggaccgt gcggaagcgc gcctggcgga
     4201 agatgaaatg cagccgctgc acagcgaccc cgtgctcacc cgctattact cacggcagtt
     4261 cctgctttcc ctggacaagg cgctggcccc ccagatcaat tgccgctacc agactgccgg
     4321 cgaatggatt cacgacattt gcgccatttc cggggaagaa tcctccgcca ctattcaagt
     4381 cctcccggca gacctccgcc atgcccatcc ggaaggaatg ccaactcccg gcgccgggaa
     4441 aaacatcggc agcagaaagg ccgccaccgg aacggacggt cttcattccg cctcacgcag
     4501 gcagtccgca cgcgctcttc gccgcaaccg ccacatgatg tccctgctgg taggaagcgc
     4561 atgcaccatc ctgctgggag cgggagcatt ttatctgctg gattacatga aaaaagcccc
     4621 tatgggcact accatcaata ccatcaagga agccatccag cagcccgcgg caagctccaa
     4681 cgtgggagaa ccggaaccgc cgccacggga tctggcatcc atccgcattc cggaactcaa
     4741 aataacggaa gacgcggcgc tggaaccgcg gtcgctgacg gagttccgtt tgcgccttga
     4801 cgacaccatc ctgacccgtt ccgggcttcc tcccatcctg ccggaaaaac tgcgcatctc
     4861 ctgcatttat ctccaaatca ttcccccggc agcaaacggg gaaaaactgt acctcaccat
     4921 ccgggactcc aacggcgtcc tggtagaccg ctccatcaat gccgtcccct ccttcacggt
     4981 gccggggcaa tccacctcca cctttttctt tcccgcactt ccggaactgt ccacggaccg
     5041 cacctacacg tactcctttg aaaatgccgc ccaccaggct gttcccatgc aaatggcatg
     5101 tttcaaaaac gagacgaaac gcacggagga agccttcccg aaaatccgtt tcatcgctgc
     5161 tcctcccgtg ccggaaccag ccctgctcca tgacccggcc tacaaaaatc ttgtggcggc
     5221 aatcgccgcg ccctccccgg agaacaaaaa tattctcgac caggcaagtt ccatgccggg
     5281 catgctgccg gccctggagc aactttccaa agccggctac ccgatagccc agcaggcact
     5341 ggggaaaacc ctcatgtctc cggactgtcc cggcggttcc aggccggaag aaggcgtgga
     5401 atggctttac cgttccgcca caggagggtg ctatgaagcc atgaaagagc tgggcatcct
     5461 gctcatggac atcccctcct attttccccg cattccccag cagcctcccc ttgcctcccg
     5521 ggattacgcc caggcagcgc gtttcctgag aatggcggtc caataccggg accaggaggc
     5581 cctgtacctg ctttccctca tgtactccca gggctggggc gtacccgcct ccccggaact
     5641 ttccagcctg atgatggaac gcctcaatgg atcttcctac tccacggaca gcctgaatcc
     5701 caacgcgcct atcgcggcgt actggcttcc ccttcctctc gccggagaaa aaaacaccat
     5761 tctgcgcttc actattccag ggccgttcgc cccgtcccag ctcaagggcg ttactctgca
     5821 caacacaagc ctgaaggatg ccttctccat cagtaaaatc aatatcctcc aacatgggcg
     5881 cctcattctg gacatcattt ccccccagga agtgctgcct ggcaaaagct ccgctcccat
     5941 aggcatatcc atcccggaag ggctgctggc ggatacgacc ctgcctctgg aagtggaaat
     6001 tttgctgacc cccggaaaca gctgcggcgt ggtggaaatg ccccctccgg tggaaaagaa
     6061 aaactccgga attgaaatca attaagcttc accgcttttc aacgtgaaac agcaacaggg
     6121 gaaacggaaa acacccttga ttcccgcctg gggcatgcta caaaagataa atgaatgcct
     6181 ctgttttcct gcggcgcatg ctacctatgc tgaccgcgtt cctgatggcc tcctgcggtg
     6241 atcctaccgt ctcctactgg aaaaaggcag cggacatcaa tacggaatac tcggaaaaaa
     6301 gcgacgcact ggtgcaacgc ctcctgaaac tgaaaaagaa ccctaccctt ccgggcctgg
     6361 aagaaagcag cagggatgcg gctgacctgt tgcgggaaag ggacgaagaa ctggcggacc
     6421 tgagcacgaa aaacgtggat cccgccgtga cggcctatgt ggaagaagac cgcaagcttt
     6481 ttgccagagg aagggaattg gcggaacgct accagcaata ctttgaaaaa tatcttaagg
     6541 gaggaccgga ttttactccg gatccttccc gggccgtcgc ccatataggc cggggccggc
     6601 aggaaatacg caaaattctg gcagaagccc gaaaactgga agctcgggct gaaatgctga
     6661 gaaaagaaaa atcagctgag cttgagcaag aacttcctcc gctgcacttt cgccttccgg
     6721 agttgaaaca acttctatcc agccgttaga aaacgcgtac gaaccatttg catcaatgcg
     6781 gagtcacggc cagtctgaca gcggctttta cactgcacgg acggacacaa ccggttcctg
     6841 aaactttccc cgtccatgtt acggaaatat cccttccctg gatttacagg aaaaacgagt
     6901 ccccctgctg tccatcgtcg tttatttcag cattcaaatt tcattccctg aatacccctt
     6961 agcacaggct tcatagttta aaattccaaa gaagggtttg cctcccctcc acaaaaaagc
     7021 cccgttcata tttcatgaac ggggctatat taatttttca aattcacctg ggagtttgat
     7081 caatgacgat aaaatccttt ttccccacat actccctccg ggcatccgga tctccatatc
     7141 cgtcccggaa acgtacttcc aattcgtata tttccccgct ggccagaggt tgtaaacagg
     7201 tcagcagcag gtatgcatcc ttgacgggca cttcatgttt ctctccgggg ttcagggtaa
     7261 taaagcgcat aagattcgtg acacgctcca tttgatagga ggtaagatac catgccgttg
     7321 gcctttttcc cttctttccg tttccctgac tccatatctt gatgatcata ttcggcccgc
     7381 gttccttgag aggatagaag tcgtcgaaaa gacgcacagc cttttctccc ttattggcaa
     7441 acaccagctt gaagtccgga aaagcggctc ttgaatcgaa cgaagccttt tccatcacca
     7501 cggaacattc aatcccgtta tcggccatat cccccccatg cgggagcatg gaagaacagg
     7561 ctggccaaaa gccctaaaat ttggaagatt ttttcattgc agcgcgagct tttgggagaa
     7621 ggcgagaatt cgcgcttcat cgtcgagttc ccttcgtact tcccttctcc tggataggtt
     7681 ccctgtccat cgtgacgttg aacgcgaccg cattgccact agagggatcg taagcgttat
     7741 tctcatactt taaagccacc ttgtaataaa atgtctcact atctccaggg cgattgatcg
     7801 accacggaga aatcttttct tcctacatac ttcctccgaa caccgggatc tccatatccg
     7861 tcacgaaaga ttacttccaa ctcatatttc tcaccattgc gaagacggcg tgaaggccca
     7921 aaatagtgca tcaacagata cgcatctttg atgagcactt catgtctttc tcccggcttc
     7981 aacgtgatga agttcaagaa ctgtcctcgc tcaatgcaat aatgagggta atatgctgct
     8041 accttttcct ttttctcttt tgtcaatttc caaaaaatct ctatggaaat atgcggaccg
     8101 ttatccttga gaggatagaa gtcatcgaaa agacgcacgg tcttttcgcc cttgttcgtg
     8161 aacaccagct tgaagtccgg gaaggcagcc ctcgcgtcaa actgggtctt ttccatcacc
     8221 acggagcatt ccactccgtt gtcggccatg tccccccatg ccgcagcatg gagggacagg
     8281 cttgccagaa gtattaaata ccggaagatt tttttcattg cagcgtgagc ctggccgttg
     8341 cccggagaac ccctaggaaa caatccttac cctgctgatt atagtagcgc gtttcgagtt
     8401 cacaagaaca ttgtttccag ccttccagat cgacttcatc cagacacacc ggaatcgtga
     8461 cgctgtattc ctgaccgggt gcaagctccc ggtagttcaa gggtcgctgg aaggtcatgg
     8521 tgcctatttc cctgttcaga acgcgcattt tcccctgttg cgtcctcaac accgtcacga
     8581 aaaagaaatc ggacgtttcg tcgttgattt catcgggaat gcggacaggc tcgtttcctt
     8641 cgttgcggaa gcggactgtg attttgcctt ccgtgaattg ggaagtatcc gccatggaga
     8701 gggtcaaggg cgccggttcc tccgcaacag ccatgctgcg ggtttcacct tcgctgaccg
     8761 gaggtatgaa acagggaggc tcgatcgttc caggtttcgt gataaacctg ggcacattgt
     8821 tccaccccat cgcgttggac agacgaatga cataggcggt tgcctcgttc aggacttcct
     8881 gtccggcgca atagggtctg tcattggcat catctggcaa cgccagtaca taggctgatg
     8941 tctccgtcca aagatccaat gccatttcat tggcaagagc ctgcatgaac ttcacggctt
     9001 tatccatgtt cggttctttg tcgcaggaga tagtctgctt ttcaagcatt tcctgtattt
     9061 tggagtcctt ccagtagaac ttgtaagcat cctcccattg acggcggtgg tgttcttcgt
     9121 gggcgaggga agcctgcggc gtgtgccatg tgcccactct tccgcgggcc tggtagccgt
     9181 tcatttcgtt gaccgcttcg acggcttctt cttcattgag agggagatca acatagggat
     9241 tcacgtaaat gccttcatac atagttagct tggctccggc agaaacggaa acaacacgac
     9301 acctccatac gttttccgat tcatctttgc aaaccgttac gcaaatctta ggtctcaagc
     9361 tacttacatt gcagtcacca ttcccttcca aatagacggg ttccggtttc tcaaattcca
     9421 gttccacttc cttggatggc tcacaggtga tagtggattc cttttcaatt gtccacaatt
     9481 tgattttggg ggacggcgag aattcgcgct tcatcgtcga gttgccttca tacttccctt
     9541 ccgtaatagg tttggtatcc atggtaacgt tgaatgcaat ggcattgttg ctgacggggg
     9601 tgtaggcgtt gttggtccac gacaaggcca ccttgtaata gcccgctttc aggtacctga
     9661 taccagttgc cagattcagc ttgccgttgg gcggtgtccc atcctttttt atagcttgct
     9721 caccggcaat cgtcaggctg cctgtgtcgt cggcctccac gccgaaatag tacaagccgt
     9781 cacgctccac cttgatgaac atttcgggag ctccagtaca ggtggcataa tccgaaggag
     9841 ttccgtcaaa gccatatatc atttcatcaa cgccaagcgt cttgttgata agaggctcat
     9901 acccttgata gatgcagacg ggattaactg ccttgttgat tggtatataa ttgttcattg
     9961 ctattgttgt tatttacttt ctggaaaaac tgaaccatca gccttccggc atttctgcgg
    10021 cattacctga acccatcaat acgtttaacg cgtagtcccc ctcattcgga acgaagagga
    10081 acgacctccc ctctaatttt acgtgctgcc gtgaacttgc aaaaaaactc ctggtgcgtt
    10141 tttatatcct gattcacgcc attttggaac aaggttgctc catatagatc tgacaacagg
    10201 cacgggcggc attccgccgc acaatcatga tgccttaaaa aacaagcatt ctcctccttc
    10261 tctgtccgtt ccgccaaaga aaaaggctgt ttccagagag tccttcactt aaaaacaaag
    10321 catcttttcc aaaaacgaga ttgacacccg cggttctttt ccctagtatc cgcgcacagc
    10381 atcagcggcg gggtagccaa gtggtaaggc gacggtctgc aaaatcgtta ttcgcgggtt
    10441 cgattcccgc tcccgcctcc aagatgttgt caaagccggt tcaagtcgcg ttcgtctagc
    10501 ggtctaggac tcccgccttt cacgcgggca acacgggttc gagtcccgta cgcgatgcca
    10561 gcttttcttt tttccgactt gcaccggaat cttttttacg ccatccccgc gggatgattc
    10621 tggatttttt ctcctgttgt ggtacagtag cttccgtcat ggcagcccag aacgatatta
    10681 tgctgaacgg agccccgtac gaactggcgg aaccctgttc cgtctcccgg cttctggaac
    10741 ttttgaacat gaaaggcaaa cccgtcgtcg tggaacacaa cggggccgcg ctcctgccgc
    10801 aggatttttc ccgggtgacg gtttctcccg gcgaccgggt ggaaatcgtc tccatcgtcg
    10861 ccggcggcta gggttccgct ttctggaaca gtcttttcca atccgtcatc gccatgccat
    10921 ccatccacgt catgtctccc acccttgcca gccaagtggc ggcaggagaa gtagtggagc
    10981 gccccgcctc cgttgtgaag gaactggtgg aaaacagcct ggatgcagga gccaaattcg
    11041 tacgggtgga gatacgccgc ggaggcgtgg gcatgatcaa agtgacggac gacggcagcg
    11101 gcatgtcacg ggcagacgcc gagctgtgca ccaaacggca cgccaccagc aaactttctt
    11161 ccctggagga gctttttgaa atcacacacc tgggattccg cggagaggct ctgcccagca
    11221 ttgccagcgt ttcccgcttc aaactctgta ccaggcagca acaggatctg gaaggctggg
    11281 aaatacgcat tgacggcggc ctggaacacg aaccgagaag ctcaggcgtc tctcccggca
    11341 ccgccattga ggtggccgac ttgttttaca atactccagc gcgccgcaag ttcctcaaat
    11401 ccgcagaaac ggaagcttcc catgtggagc accagatacg cctgcatgcc ctggcttatc
    11461 cccaagtacg gtttgcctat aagcgggacg accagcttgt tttcgacctt cccgccacag
    11521 cggatctgcg cgtccgcatt tccgcactga cggatgctgc cacagcagcg gccctgattc
    11581 cgatagaaac gaccatcggc cccggtattt ccatcacggg attcctgctt cctctttccg
    11641 aagccagacg caccaaaaaa gggcaatacg tctttatgaa cacgcgcccc gtggaggacc
    11701 agctcattaa cagggccatt cgggacggct acggaggctt ccccaccgga ctgcacccgg
    11761 cgcttttttt gtatatggag gtggagcccg ctctggtgga c
//
LOCUS       DAWEMX010000078        11710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2319_length_11710_cov_10.763621, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000078 DAWEMX010000000
VERSION     DAWEMX010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2319_length_11710_cov_10.763621"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1751..1760
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2764..2844
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3377..3386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2195 a         3304 c         3665 g         2445 t
ORIGIN      
        1 cctcgcggag aaatgaccgt gctgaccggg ctttccggct ccgggaagag ctccctggct
       61 tttgatatct ttttcgcgga agggcagcgg cgtttcatgg acgtcatgtc cccgtacgcc
      121 cggcaattca cggaacagct ggaaagtccg gacatcgacc gcctgaccgg attgcccccc
      181 acggtggcta ttgaacagaa catgtcccgc gggggcacca aatccaccgt gggaaccgtc
      241 acggaaatct ggcaattcat gcgtctgctg tatgccaagc tggggcaggc ctattgcccg
      301 cagtgcggtg ttccggtggg caaaaggtcg gaatcggaag tggtggagct ggtggcgagg
      361 gaactgaaaa aacatggcgg actcgcgttg ctggcccccc tcgtcagggg acgcaagggg
      421 cattacgccg acctggcccg ctgggcggaa ggaaagggat atgaggaaat gtgggtggac
      481 ggcaaactgg ttcccctgag cggattccag ccgctggacc gctattccag ccatgacctg
      541 gacctggtcg ttgcacggcc ggaggcctcc tggccgccgg aaaagcttgc cggggcggtt
      601 catgccgcgc tggagatggg ggaagggttc ctgcaggtgc tcccgcccgg ttccgtccgt
      661 ccggaactga tgggcactag gctggcatgc gcctcctgcg ggcaatcttt cccggaactg
      721 gaaccgtaca cgttttcctt caactctccg cgcgggtggt gccccgtatg ccgcgggcat
      781 ggaatcgtcg gcagggggaa agtaaaggaa gaccaggcgc aatccctgct ggaggctgaa
      841 ctgaaatatg acagggaact ggcgcggcag gcggacgatg acaagggaat aacaacctgc
      901 cctgcctgcc ggggcgtgcg gttgaatgaa tttgctcgcg ccgtgcggct tcagggagtg
      961 actcccggtg acattgcctc cctgcctgcc gtggccgccg cagaactggt caaagggtgg
     1021 caatttgaac gggaggaagc gctcattgcc cgtgacgtag tggcggaaat cacgcagcgc
     1081 ctcaacttcc tccagcgggt ggggctgggc tacctttccc tggaccggag cgccacgacg
     1141 ctctccggcg gggagaccca gcgcatccgt ctggcttccc agctgggttc ccacctccgc
     1201 ggtgtgctgt acgtgctgga tgaaccgacc atcggcctgc atccgcggga caacgaattg
     1261 ctgctgggca cgttggatga actcaaacgc cggggcaaca ccctgctggt ggtggaacat
     1321 gatgaagata ccatgaggcg cgcggaccat attgtggaca tgggaccggg agccggcatt
     1381 catggcggca gaatcatggc ccagggcacc tttgaggaac tggccgccat gccggactcc
     1441 gtcacgggcg cggccctgca ccataagccc aggcatccct accgcgggaa aaggcgcagg
     1501 attccgtccc aaaaggatga atccgcgtgg ctgcgcgtgg agggctgctg cctgcacaac
     1561 ctgaaggggg tgaacgttgc cattcccaag gggaggctga cggtgctcac cggcgtatcc
     1621 ggagcgggga aaacttccct gatgacggga accatccgtc tggctgcgcg ccaggccatc
     1681 ggcgacaagc tcacgaggga gcagagaaaa ttatggaaaa cctcttccgg ctttgactcc
     1741 atccgcatgg nnnnnnnnnn ggtgtacggc gtggaccaga accccatcgg caaaacgccc
     1801 cgttccaccc cggccaccta cgtgggtttc ctggacgaca tccggaccct gttcgcgcag
     1861 acggcggatg cccgcaggct gggctttgac cgcggccgtt tctccttcaa tacggggcag
     1921 ggcaactgtg aatcctgcaa gggcacgggc atgcaaaagc tggaaatgga cttcctgccc
     1981 ccctgttatg tgccgtgcga gacctgccgg ggcaagcgct acaacgccgc caccctgacc
     2041 gtgaggtaca aggggaagac cattgcggac gtgctccaga tggactttgc ggaagcggcg
     2101 gaattttttg aaagccagcc ccgcatttcg gaccccctca aactgctgtc ggaaaccggc
     2161 ctgggttatc tgacgctggg gcaggcctcc aacacgcttt ccggcgggga agcccagcgg
     2221 ctcaagctgg tgacggaact catcaagggc cggcgcgtca gccggaacgc cctgatgaaa
     2281 ggcagggaac tgcccggaga cctgtacctg attgaagaac cctccatcgg cctgcatccg
     2341 cgggatgtgc ggctgctcat tgacgtgctg caccgtctgg cggaccaggg gaacaccgtc
     2401 gttgtcattg aacataacac ggaaatcatg gcggaggcgg actacgtcat cgacatgggc
     2461 cccggacccg gcgatgccgg aggaaccatc gtggcggaag gcacgccgga gcagatagcc
     2521 gggaaaaaat cccccactgc ggaatacatc cgccgggaac tgaatgggga gtcttaggca
     2581 ggaggaagga tcaataaggc actcagggcc gttccgggaa tgccggggcg gccgtcgtat
     2641 tcgcctgcct ttttctgttc cgtgaaaaca ggcatggctt gcgcccggca taaaagaagt
     2701 ttggcggaga gagtgggatt cgaacccacg gtgggttgcc ccacggcggt tttcaagacc
     2761 gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnngaggct gatgagccgc atggcgcatt tccggggagt
     2881 gtctgcggaa gtgtctatgg agaaatcatg cgggaaatgg cggtggatgc ctgcgtgctg
     2941 gcgctgggca tgggccgggg caggagtccg gtcagtgcgg cgccgctcgc gctccagcag
     3001 aacatcctgg cggcattcca ccctgaccaa gatgcggggg atgccgtcca ggcggttcag
     3061 gaggtcctga atccagcggc gggattctcc gatgacgtgg tccacgatga ccagggcgcc
     3121 tttccgggcc gcctcggcaa tggcggcgtg gaaagattca atgaagggca ggccggattc
     3181 cctgacggca tccagggcgg tttcatgctt tccccggctc atggccagat aatcgtccat
     3241 ggagaaaatc atagctgcct ctttcagcag aagctgcaag ttccgggcca gtgtgctttt
     3301 tcccgcgctg gaggctccgt tgagcaggat gacgcgcccg cttcgcggcc ggggcgcttt
     3361 ccggttctcc gcagggnnnn nnnnnngacg gttcgcggag gtgttccggt caccgtttcc
     3421 cggccggcag gttccgtccc cttccgtaag gctgggggct tcctggcggg agaggcaggc
     3481 tggtttccag aggatgtcgg acgcggcggt catggaaggt tcatgggcat catgaataaa
     3541 aagctccgtc agggcaatca taagaatggc cctgtgcgga cggaatcgct gctgccgttc
     3601 atgtcttgcc gggcagacgc cgggaaggat tttcttgcgg agcggctgcg gcggcatgga
     3661 tgccgtccgt catgactccg tggggcatgc tgcgcctttt cccgtcaata acagggatgc
     3721 ctcattcctg ccggaagctt tcccggccgt catttgcttc ttgcagcgga cgggggaggc
     3781 atgagagagt tcgtcatggc ctcttccccg gatgtagtag agtatatttg tttccagctc
     3841 cgtcatgccg gggatatttc cttcagaaaa atgtttgggg actacggatt gtactgcggc
     3901 cggatattct tcggtttggt gtgtgacaac cagttgtatg tcaagataac ggagccgggt
     3961 ttgaagatgc ttccgcaaca ggagcagggc tgcccctatc ccggcgccag gccgcatttc
     4021 ctggtgacgg atctggacga tgaccgggcc ctttcctccc ttgtcaggga gacatgctcc
     4081 gttctggctg ttccgtctgt tctggagggg aagaagaaaa aggcgtccgg tgaaaaaaga
     4141 agcaggcgcc cttagacgcc tgccggcggg aaatggctgc tggaggggaa ggttattttc
     4201 cgggagtttc cggagcggcc agagaggaga ggttcttctt caggctggcg gcggcggttt
     4261 gcgtggcttc gttgtcggac tccgtgaatt tgtccacggc ctgcgtcagg gatttaaggt
     4321 cggattccga tattttgcgc ccttttgcct tgcgctgttc cagagcggag atttttttat
     4381 taaggtcggc cagttttgat aaatccctgt tcgctttcag ggccgccaga ggttcgtcaa
     4441 attccttgac ggagggaacc gccgttttga ggcgcatgat ggccgttacc gcttccagag
     4501 ggtcggactg gatccgggcc tgcacgattt ccttggtatt ttccagccat gcgtcaaaag
     4561 cttcacatac ggcctgcgct tcctcgtcat tcgttttttt cctcaggggt gtgattttgg
     4621 attcgatgtt gctgccggtg gaaacgaccg ttttggccag cgatttgtat ttgttcagtt
     4681 ccactccttc cagaatagga aggccgcgtt ccatcttcat cacttccttt ttcaccaggt
     4741 catagagctg gggaggatag cccttggcca cgacttttcc gttagctcca atcagaacgg
     4801 catgcggcaa tccgcccggg cagggagcct ccggaatgct cagactctga taaatgggga
     4861 aagtgatctt cttttcttcc agaaactgtt tgactctggg ggacgggagc tggctgtggc
     4921 tgcctatgac agtaaagccc ttggactgga atttttcctg caattcctgc agatgcggca
     4981 tgctggcgat gcagggcggg cagttgatgc cccagtattc aaaaaacacc actttccctt
     5041 tcaggtcgga agttttgagt ttcggcccgt ggatatggct gccgtcgttg aaggctggat
     5101 aggtgacttt gctttccgcc gcctgggcgg aggcgggaac cgacatggcg gcgatgatac
     5161 cggataaaat aaaggcagga aaggcgttca tggaacggat gatgaaggac gccgcattga
     5221 gtgtcaagga ggaagcggaa tttccaaccg gtgtttccga agggttcaac ggcccgcatg
     5281 cgccggggat ggggggctgg ccggggcact ccctgccctg ggaagatttg gctgtttttt
     5341 ccctgaggga gaggcgtttc aggctatatc cagtcccatg atgtagtccg tcatgtagta
     5401 accgtggccg atgtggaaat ccccgcgttc cagggtccgc atgcccatgc gctggtagaa
     5461 ttccaccgcc cgggtgttat cgcggttcac gttcaggcgc atcaggcacg gttccgggtg
     5521 gatgcttctg atgtagctga tggcgtggcg gaacagaaag cttcctatat gcaggccctg
     5581 gaagccgggc agaacgtaga ttttctgcaa atggaagacg ccggggcctt ccggctggac
     5641 ggacaggtag ccgcagtcct ttccctcatg ggaggcgatg aagtacacgt gcccctcctc
     5701 catctgtttt tccagattgg cgggggaata catccagtcc atcatgaaat ctatctgctc
     5761 tctggaaagg aggtcccggt atgtggccgg aaaagcctgg caggccagtt catggatcag
     5821 cgtgatgtcg tccttggcgg cttggcggag agtcggcatg gcagaaatat tacacggaaa
     5881 agcgccggat gaaaagggcc tcttccccat ttccggcgga attttccggc atggcctatt
     5941 ttcccctacc tattccggcg gagacatcct atgctgaatg ccgccgtttt ctgaatgccg
     6001 ccgtttttct gacagcctcc cgggaaatgc tgtttttatg gcgaaccctg catgtccagt
     6061 catgaaagaa cccattccca tacaacaatg gcttcccgcg gggcctctga gggacatggg
     6121 tgaaaaatac gtaagccagc ttccggacgt agcgcaaaat cccatcggtc cggaatccct
     6181 catgcaccag tcggaccatt cctggtcgga atacctggta gcctattccc tcctgtaccc
     6241 gggggtagtc atcatcctgg ccctgctggg ggggctgggg cttggggctt tcttcatttt
     6301 ttgccgccgc cgggaatatt cccaccgtat tttctgttcc aaatgcggct ccatgatgta
     6361 cccgtgcggg ctgcactgcc cggagtgcgg cacgtccaat ccgtcccccc gtgccttgaa
     6421 ctggatagga tattccaggc tgcgcaccgt ggttcctccc tccggatgga aaaggcatga
     6481 ggaagttctc cgcagttacc ggcggtgttt ttactgcggg cagcctctcc gtgagccctc
     6541 tcttgaccag cgttgccctg cctgcggcaa ggcggtgctc cagggtgagc agtccgtggg
     6601 ccggtatgat gcgtatattg gccgccgcag ggggtggacc ttcgctgccg tggtcgtgct
     6661 gggggtggtt cccattttgg ggccgctgct ggcttcctcc ctgtacagga gaacgctcat
     6721 caacccgtat tccctgtaca tgaccgtgta ccgggaaagc ttcctgatgg tggttctttt
     6781 tctgtgccgt catttgtttc gcctgctgcc gttcatcggc atcatcggca tgcccgtcct
     6841 ttgcgtgacg gaataccacc tgtaccggcg catgttcctg tggaaggcgg agaaatatga
     6901 tttcaggggg gaatgaagca ggagaccctc ttccggtaat gcctgtccgg cgcctgcgcg
     6961 gaaaatcatg gggtgcgggg ctgttggggc ttccgggcag cgtatcgttt ccggcagccc
     7021 gctttatggc gcgcggcggc aggaaatggg gaataaagga tggccggctc cttgattgac
     7081 gcgcagcagg ggatgcggag ttgatggaga gatgttctca atgaatgttt tacgtcttat
     7141 tgtatcaagt atgacaagga attgtttttt tcttatggac taaacttacc tggtacagta
     7201 tgtaggcagt gttccccgga ggggggctgg atgattccca aacccccgca tgcggaggaa
     7261 caaaaactgt aatataaata aaatgaaaat gaaaagaaaa ctggcatcgg ttctggcgaa
     7321 ggctttctcc ggaaagtccg tgccggaaaa gaaacgggaa agggaggcca gggcctccaa
     7381 aaaactggaa gcgtggctga aggcttccag aatgcgctcc ttttaaggaa gcgcccgttg
     7441 aagggaaggg cggattcggg cgccgggcgt ccggttctcc ggctgggacg gggatgttat
     7501 ctccgtcctg ttgtttttac cggaaaaggt atgtcttgat gacagttgcg ccttgactac
     7561 ggcggtggaa tgtccgttca tctgggcaga catgaccata cccggaattc ttgaagaaaa
     7621 gttgtcctcc gcattaaagg cggtgttggg ggaggagctt cccgctgatt ttcgcgcctc
     7681 ggtgacgccc tccgcggatt tgcggttcgg ggattaccag agcaacgccg ccatggtgct
     7741 tgccaagcgg tgccgcacga atccccgcgc gctcgcccag caggtggcgg accggatagg
     7801 gcaggatgcc gtgtgctccc tggagattgc cgggcccgga tttatcaatt tccgcatcag
     7861 gccggaattt tacgcagcgc gcctgctggc catgctggcg gatgaccggc tgggggtgga
     7921 aaaagtgcag gcccccaaaa ccattgtcat tgacttttcc gctcccaacg tcgccaagcc
     7981 gatgcatgtg ggccacatcc gctccaccat tattggtgac gccctttccc gcgtggcccg
     8041 ttttgtgggg caccatgtga ttacggacaa ccacatcggg gactggggta cccagttcgg
     8101 catgatcctg tggggctgga agaatattct ggatgagcag gccctgtccg ccaaccctat
     8161 tgacgagctg ctgcgggtgt acaaggatgt gaacctgatg tgcaaggaaa agccggaact
     8221 gctggatacc tgcaaggcgg aactggtgaa gctccaggcc ggggacgggg aaaacctggc
     8281 catctggaaa cgctgcgtgg aagtttccaa atccgggctt tccaaaattt acgaccagct
     8341 tgacatccat tttgattact ggctggggga aagcttttac aatgatgccc tggccccgct
     8401 ggtggacggc atgatcgccg ccggaatggc ccgggagagc gacggggcca tctgcgtgtt
     8461 ttccgacggt tccgtgccgc ccaatgaaga ccccttcctc gtgcaggaca agggggaatg
     8521 gcgcgccaac ccctgcatca tcaggaaagc ggacggaggc ttcctgtacg ccaccacgga
     8581 cctcgcgacg ctggaccacc gcatcaggac gtggggcgcg gattccatct ggtacgtggt
     8641 gggcgcccct caggccctgc atttcaggca gattttctcc acgcagcgcc gccgcggcat
     8701 ggacggggac taccggcaca ttgcgttcgg ctccattctg ggtgacgacc gcaagccgtt
     8761 taagacgcgt tccggggata cggtttccct ccaggatgtg ctggatgaag ccattgaacg
     8821 cgcggcccgc gtggtggagg agaagagccc ggacatgccg gatgaggaaa agaagcgcgt
     8881 ggcggaagtt gtgggcatag gcgccgtgaa gtttgcggag ctgtcccaga accgcatgac
     8941 ggattacgtt ttcaactggg acaagatgct ggctcttcag ggagatacgg cgccgtacct
     9001 ccagaactcc tacgtgcgcg tccgctccat cttccgcaag ctggacggcg ggcctttgga
     9061 ctggtccgcc cccattcagc tttcagaaga tgcggaaatc catctggccc ggcttctggc
     9121 ccgctacggg gaagtggtgc cgcaggtgct ggacgactgc cgtccgaacg tgctggccgc
     9181 ctacctgttt gacctggcca gggctttcca ttccttctat gaggcgtgcc cggtcctgaa
     9241 gtcggagggg acggtgcggc actcccgcct ggccctgtgc gagctgaccg cacgcacgct
     9301 gaagcacggg ctgggcctgc tgggcatcca gcttccggac aggatgtaac cgtttctccc
     9361 gtatggacgc ctctttcctc catgtcctgc tggccgccgc cgtggcgggg tgttcttccg
     9421 ccgccgcgga cggggcacac gggtattgga tgaacagccc gtccctgaac ggctggcggg
     9481 aagccgtctt ccgcgccggc gttccggcgc agcctgccgt gctgccgccc ggcgtggagc
     9541 cgatgatggt gggttcctca tctcccgccg cgcaaatgct ggtgctggag ggaatgacgc
     9601 atctgctcac gttcggggac atgagggctt tccttaaatt tgacgccgct ctccgtctgg
     9661 acccggactg cctcatggcc cactggggca ggtgcatgag cctgatgggc gcggggcccg
     9721 ccttccagga ccagcgcgtc cattccatga agcgcatgaa agagctggcg ctgcgcccgg
     9781 actgcccgga acgggaacgc gcttatgcgg atgccctggc cgtgctgctg atggatggcc
     9841 ccgtgaaggc gcgggaagcg tggaaaacca tctgttccac ctggaagcgc gacccatacg
     9901 cccccctctt ttacgccatg ctcctccggg acggctttga cgggcagggc aaccccggag
     9961 aagggcagaa ggaggccgtc cgcgtggtgg aagacgtcct gaaggaacgc cccggctccc
    10021 aggccgccct gttcatgcgc gccctgctgg aagaggtggc gccatccatt tctccggcaa
    10081 cggtggaaac cgcccgcagg gccgtgtccg ccaacccctt ttccgcatcc gcccatcatt
    10141 tactgggcca ttgcctgttc cgcacgggag attatgaagg ggcatccgcc gcgttcaagg
    10201 agtctgaaaa tctgtgcctg gcctgggaga aggcggaaaa cgtgtctccc gcgcttgatg
    10261 acgcctactt ccgttccatc ctttaccgtg ccgtttccga attctgcgcc ggccgttaca
    10321 aaagggcgga agccatcgcc tccagagccg cttccgttcc cctggacaaa aagcatccgc
    10381 tggctcccgg aaccctcctt cagctgtggg aggccaggac gctgcctgcg cgcctgatgc
    10441 tggcccggcc cggccttccc cggcaggccc ttgtgctcaa ggcttttccg ggtcctctcc
    10501 ccaaagggtt tccggatttg agcaacggca tgaccgcagt ggcctcccaa tacatgggcg
    10561 catgctacgc cgcaaggcag ggcagaacgg acgccgtggc gtccgccttt gacaagatgt
    10621 ccggcattct gcggctgctg atggacggcg cggacgccgc ccggaggcag atgagcgtct
    10681 cctactgggc gcgctgcctc caggcggcta gcctgtattc ttctgaaatc cgctccctca
    10741 tgttcccgga ttccgccaat gtctggatgt ccgaggccat acggagtcag cgtttttcct
    10801 ccctgctcct gcctcccgta gtgccatacc ctgcggaatg ggtgctggcc cgcgcctacc
    10861 tgaaggccgg aaaattccgg gaatgcgcgg atatgtgcga acaggcgctg aaacgcttcc
    10921 cgaaccatgc cggagtgctg gagaccctgg acaaggcccg ttcttccgga aaataattag
    10981 gaataaacgt gggggagcgg ttttgatgcc cggtttcctc atggatggat tggagatggc
    11041 attccccctc atcacgatgg aacatgacgt ccaatcccgg gacaggaagc ttgttcatct
    11101 tgaaaaataa gcctgcggtg ataaaaaagg ggcgtgaagc cgtgcccgga aggttgcggg
    11161 ttccttctcc gccgctatga agatgttcgg tccgcatgcc ccggaggccg gatccgccgc
    11221 agcattcatc aagatgctca gcaggttgcg gctgttccgc cggatgttcc ttaaacatgg
    11281 taagttttat tttttcatgg ctgttgcggc aaataccgga acgcgtccgt tgtcattttc
    11341 aataacatca tacatcaagc aatatgaacc atccctatct ggacccctcc tttctggttt
    11401 cctggtcccg gcttacgccg gaggccatca ggccggacat cacggaagcc atctcccgcg
    11461 ccaaagagaa tatccggacc atttgcgacc agccgctgga gtccctgact tatgaaagca
    11521 ccttcggcgc tctggaaaag gcctccgagg atctgcacct gggctggggc cgcatcatgc
    11581 acctggactc cgtcaatgac gaacccgccc agagggaggc cataggggaa atgctgccgg
    11641 aagtggtggc tttctcctcc tccgtgccgt tgaacccgcg cctgtggacg gtgctgaaag
    11701 ccgcggcctc
//
LOCUS       DAWEMX010000079        11614 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2343_length_11614_cov_10.085215, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000079 DAWEMX010000000
VERSION     DAWEMX010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2343_length_11614_cov_10.085215"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11305..11314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2850 a         3480 c         2818 g         2456 t
ORIGIN      
        1 gcgaacgccc tcaactccat ggcggcccag atcaagcagg ccgccatgaa aatggcggaa
       61 catcattgat tttttccaga gcaatgactc acggcctttt cccgcatctt ctcctgctct
      121 ccgcattctg cggcgcaagc ctctcatgca gcagttcttc ccgggacatc accatccgca
      181 gcattccttc cggagccagc ctgcgcgtga acggagacta cgccggccag gcacccgtca
      241 cgctctccct gaacaggcac aaacccgtgc atgttgcggc ggataaaccg ggcttccttt
      301 ccacggaaaa aacgttttat ccggaaatga ccacgcaagg tgccattttg tggggccgca
      361 acaacgagaa gtccaaagac tttaaaacag acatcctgac catccgcctg aaaaaggcgg
      421 actcaggagc gccccccttg cgggaacttc ccgcgcagtg gtaacagcct gccgtccgga
      481 cactttccgg gagtaaaagc gcatatccat cccgcgttcc gtacgcagct ccgcactacg
      541 ctccctttca caaacggata aataaaccgc ccgtttttgg aattgcacgc taatcctccc
      601 gccccgggtg gcgggccatg acggcggaac ctaccctctt caggaccagg gccgtacgcg
      661 gtagcaggta cagggaaaga ttcccatccc catccgtaaa atgcggcatg gaaacatcct
      721 gcctcccgaa accgccgaaa cgggcgttgt ccgtatccag cactacccgc cattccccct
      781 tctgtggaac gggcagcatg taatccatga tagccctgtc tccggaccag ttgaacacaa
      841 acaacagacc gccgcggtgg aatgccatga catggttggt ctcgtcaata ttaagcggga
      901 aaggcggcgg attattcagc agccgagctt cctgcgccag gcggaccatc gcctgatcaa
      961 aggcattcag gaacttgaac ctgagggaag gattgtccac cagggaccac tggcggcggc
     1021 agtattcgta agaccagccg ttgccttcgc gcggaaaatc aatccattcc ggatgcccga
     1081 attcgttgcc catgaaattc agccagcctt cccccccggt agccagcgtc accaggcgga
     1141 tcatcttgtg cagcgccatg ccgcggtcaa taatgaggct ctgctggtcc acggccattt
     1201 tccagtacat ttccgcatcc atcaggcgga acgccagagt cttgtccccc accagggcct
     1261 ggtcatggct ctcgcaatag gccacatgcg gctcgccgta gcgcctgttg gtcagcgtgt
     1321 gccacatatc gcccatgctc cattcctcat cctttttctc cttgagcagc ttaatccagt
     1381 aatcgggaat gcccatggcc agacggtggg aaaaaccaat ccccccttcg tccaccgggc
     1441 ggcacaatcc cggcatgccg gacatgtcct cagcaatcgc tatggcgccc ggcttcaccc
     1501 gctgaatcag cgtggcggcc agctgcagat aagccacggc atccagatcc acggaagagc
     1561 cgaagtaggc gcccaaatcc ccgaacggct catgcccacg gtggaaatac agcatggatg
     1621 tcacgccgtc aaaacggaag ccgtcaaaac ggaactcttc cagccaccag cggacattgg
     1681 acaggaggaa ttcaatcacc tcgtcccggc cataatcaaa acaacaggaa tcccagtccg
     1741 gatggcggcc gcgctcccca ggcaggaaat acatgcctcc ggaaccgtca aaattgttca
     1801 gcccttccgc ctcgttcttc acggcgtggg aatgcaccac gtccagcagc acggcgatgc
     1861 ccaggccgtg cgcctggtct atcaggtact tcaggtcctc cggctcgccg aaacgggagg
     1921 aaggggcgaa aaaggaagaa acgtggtagc cgaaggaacc gtaataggga tgctcctgga
     1981 tcgccatcag ctggacggta ttgtaaccca gcctggcgat ccgggggaga acctcatccg
     2041 caaactcgcg gtacgtatgc acgcgccctt cctccccgcc catgcctaca tgcgcttcat
     2101 acacgaacgg aacttccacc cgggagggat caaaaccgtt attccgccat tcataggggt
     2161 gttccggcat ccagatctcc ccggcaaaat cataagtggt cggatcctgg acggtcctgg
     2221 taatccatgc gggaatgcga tctcggcccg tgccgtccgc accgaccacg tgcaccttca
     2281 ccttctgccc gtgggccagc gcatcaggcg gcagcgtaat ttcccacacg ccgcgctcat
     2341 tccgctccag cgggtggctc tcccggttcc agccgttaaa atccccaatc agggacaccc
     2401 tgcgggcggc gggagcccat tcccggtatg tccacgcccc cgtctccgcg tctctgttga
     2461 aaccgtaata gcggtatccg cgcgcgtatt cctccagaga gccggcgcgc tgattgatcc
     2521 tcttcatttt caggtcaaac agacgctggc ggtcgcgtat ctggcgggaa tacggctgga
     2581 gccatccgtc cgccatcacc aagccgggta tcgggggtct tctcattatc tgatgatacc
     2641 cctcctcaat cagcagggtc aaggaagaaa gaagggcttc tccctgcggg ggaagaaaag
     2701 ggcgcggccc tttaatcgcg cccgggaagg cgccccgtat cacgcatgta catcatcctg
     2761 gccagaacct ccttggaaat gtggttcaaa tcaaaatgca cagggggaga atccggcagc
     2821 actccccagt ccccgtactg gccgtacaac accatttcag gaatcctggg ggccgggaaa
     2881 tccctcccct caaaccgaac gcgggaaagc ggaaacagcc attcatggcg gaaggaatgg
     2941 cggcacgggc cggcaaaagg aatctcaaat cccaggaaaa tgtttcctcc atccgccggc
     3001 cgccgtcctc ccatcaccac ccggtcccgg atcgccatct tttcctcata agaacaccgc
     3061 gtccggtagc ggcaggagga atctgcctca taatcaagca ttccggatgc cttgtaaccg
     3121 cgcagcagcc actccctttc ttcaggagca tccggagacc acgcggcagg ggccgtgtca
     3181 tacgggaaaa tgtccacatt gcacaacgtg cccggcaacc ctatctggat gaacggatcc
     3241 atactgacgg agaaaccgcg cgaaccgaaa agcccggcgg cgttcgccct caacctttca
     3301 taatcttcac gcagcatgga aatatccagg tcgtcatccc aggggataaa gccccggtgc
     3361 cgcaccgcgc ccagcagcgt cccgaaatca agccagtacg gataaccgcc ctcctcaaga
     3421 attccggcca cttcattcaa aaaacaggtc atggcctgct gcacaatcct cagcactccg
     3481 gacgcctggg gcatctcctc ggcccgaacg gtagctttta ccagtttgcc caattgggag
     3541 gcttcacctt cccggcccac cttgaaccgt ccgcatggaa taccgaaaaa acggatgaaa
     3601 cgctttttcc tatccgtgca ggaagaaagc actttcaggc cgcagcaccg gacttccgtc
     3661 acccaccaat gctttcccct tactaatttt ccagtccttc cggcggtaat gaatgatggc
     3721 agcttcatcg cagataaatt gggtcaaaaa cacgcaggat atgattcatt tttccaggaa
     3781 agcacatccg gcagaatcag aaccttatcc cccacttttt acaagtcaat gaaaaacaaa
     3841 ttatcctttt cggagaaaac acccgttcca tgatattaac gctgcttttt ccaaaatgca
     3901 gacagacctt atccttccct ttccacggta ttggcaacaa gcggattgag catccgccgt
     3961 caaagtaaaa atttctgtaa gaatatgtta atgggatatt acccgggcga aaagaaaaaa
     4021 ccgcctttta tgtttcatcg caatcctttt atttttcatt gatttaactt tggacagaat
     4081 gggagaaacg tttaacggat gctcaatccg ttaaacatag ttcccggttc tttctcaggt
     4141 tctgaagaca aattagaata cttatgcagg cgatcatttt agcggccgga atgggaaaaa
     4201 gactgggagc gctcacccgg aacaatacca agtgcatgat ccaggtcaac ggagtgacgc
     4261 tgatagaacg catgatgaga caattggacc gcctttcccc gtctctggaa aaaatcgtga
     4321 ttgttacggg ttatgagggg gaaaaactca aaaccttcct ttcccagctg gacatttcca
     4381 cccctgtgga atatgtggac aatcccctgt acgccagcac caacaatatt tattccctgt
     4441 acctggccaa ggaacacctg cttcaggacg atacgctgct ctttgaatcc gacctgattt
     4501 ttgaagactc cgttattgat gccctgctcc gccatcccta ccccagcctt gctctcgtcg
     4561 ccaaatttga aagctggatg gacggcacgg tcgtcactct ggatgaagac gacaacatca
     4621 ggcaattcat tcccggcagc aaattctcct acaagaaaaa gacggaatat tttaaaacag
     4681 tcaatattta caaattcagc agggaattct cccggaccca ttacgtcccc tttcttacag
     4741 cctacagcaa agccctgggc aataatgaat attacgaaca ggtgctgcgg gtcatcgccc
     4801 tgctggacaa gccggaaatc aaggcgctgc gcctcggcgg cgaagcatgg tatgaaatag
     4861 acgacatcca ggatctggac atcgcagcct ccatattcac cccggacagg gaagaacacc
     4921 tgcgcctgat ggaatcccgg tacggaggct actggcgcta tccccgcatc agggatttct
     4981 gttacctggt caatccctat ttccccaaca aacgcctcat gtccgaactc aaggcgaatt
     5041 ttgaacgcct agtgagggaa tacccgtccg gaatgagagt caacagcctg ctggcggcaa
     5101 aatacttcgg catcagccag gaatacatct gcatcggcaa cggcgcggcg gaactcatta
     5161 aggcgctcat gtcccgcctg gaagggaaaa tgggggtcat ctaccctacc tttgaagaat
     5221 accccaaccg ggtgaaaccg gatatggtgg ttcccttcca taccgcttcc cggaatttca
     5281 cttatacggc ggacgacctg atggaattct tttccgggaa agacatcggt accctgctcc
     5341 tcgtcaatcc gggaaaccct tccggattct tcctgccgaa aggcgatgtg ctacgcctct
     5401 gcctgtggac aaaaacccgc ggcatccgcc tgatcgtgga tgaatccttt gtagacttct
     5461 cctgcggaat gccggacaat acgctgctga aagacggcat cctggaagaa tatccccacc
     5521 tggccgtggt aaaaagcatt tccaaatcct acggcgttcc gggcctccgc ctgggtgttc
     5581 tggcctccgc agacagggaa ttggcggcat ggataaaaaa agacgtttcc atctggaaca
     5641 tcaattccat cgcggaattc tacatgcaaa tcttcgggaa atacgagcaa tcctacgtgc
     5701 gcgcgtgcaa ccgtttcatg aaggaacggg accgcttcat gcagaaactc tcccgcgtgc
     5761 ctttcctgaa cgttttcccc tcccaggcaa actatttcat gtgcgaggtc aagcccccca
     5821 tgaaagcccg ctccctgacc ggccttctgc tgaaaaacca ctccatcctt gttaaggact
     5881 gtagccggaa aaaaggattt gaaggcagag aattcatccg tatcgccgtc agagacgcaa
     5941 ccgacaatga tgccctggtc cgggccatgc atgcagaaac cggctcggaa ccgtcttcta
     6001 cttgtcccac taacggagaa tccccttttt catcctgttc cacttcagcc tcctgatgaa
     6061 tacaatttcc actcctgtca taggcatttg cggcggcggc aacctggccc atgccatggc
     6121 gggatggctg ggtacacgcg gcctacatgt caatatccta acccgaaacc ctaatcaatg
     6181 gggaaaaagc ctctccgcta tctttccaaa cggtactacg caccatgctc ccctagggca
     6241 tatcagcaac catccggaaa cattgaaaga ctgcaacctt gtgctggtgg cggttccccg
     6301 ctttggcatt cgggaggtat gccaccgcat taaaccctat ctgcatcgag aacaggggct
     6361 cgttattgta cccggtactc cggaggtcat ggagatggca gaagatccct cttggacttc
     6421 caccgtcagc ctgatgggaa tatacaaggt tcccctgatt tgtcggacgc aggaatatgg
     6481 acattccgtc tccattctcg gaagccgccc tcttaacagg atatgggtag ccccaggaaa
     6541 taatgccgaa cattggtccg ccatgttgga aaccttattt gacactcctc tgactcatct
     6601 ttcttctccc tggccctttc tgttgaccaa ctccaacccc cttctccacc cttcccgctg
     6661 catgagtctg tttcgccact acagggaagg aacgttttat gacaagcagt ttctttttta
     6721 tgaagaatgg acggaggagg ccagtgagtt gtacatccag gcagatagag aacttctgac
     6781 cctctgttcc tgttgtcccg gcatggaaat cggaaaggac atcattcccg tcctagatta
     6841 ttatgaatcc agaaatgccg tagagttgac taaaaagatt caatctattc cggcattcca
     6901 tggggttaaa gctcccatga cccgccggaa acaaggatgg attcccgatt tttcttcccg
     6961 ttactttacg gaagatgttc ctctgggcac caaacccatt tgctatctgg cggagcaatt
     7021 aaacattccg gttcctacac tccagtcctt tgtggaatgg aacacctcca tactgaacag
     7081 attctcccca tttcggaaga aataatagga tggcttccat tcatgcgaaa caaaacggtt
     7141 ccaagaaaaa gttccgtcgg cactacccca gaattccaca ggtgtaagaa ggcctcttct
     7201 ttctaactga tctcttccac acaataccct ctcctgaaac gggaggcaac atcagccatg
     7261 aacacggaat cctcacgggt caaaagacgg ttgccgtgct ggcagccatg aatcaggcgc
     7321 aaaaacgcgc caagttcata acgcagccca tccccttcaa aattggcaaa atatttcctg
     7381 ttgagccggg catcctcaaa ccgcacttca aacatctccg tcttccacca aggcgccggc
     7441 acataaatat acccctccgt tccggtaatg cacaagtccc cttcctgctt ggctcctatg
     7501 gctgccgtgg cggtggctac tgcattggaa taaagaaatt ccgcgcgcgt gaacacgtcc
     7561 acgcccgtat gaggctttct gcaagtaaca aaacgaatcc tgcggggctc ggttcccaga
     7621 agcttcccaa tgacaaaggc gggataggaa cccagttccg tccaggcgcc tccggcctgc
     7681 atggggtcat actccctggc agcctcatcc tctatcagct tggtaaacgt ggcgtccacc
     7741 gccttgatgg aaccaataat gccgctcccc gccaaactgg tcagttgctg gaaagccgga
     7801 caaaacgcag ttttaagggc ttccagcaaa acgactcctt tctcttcggc cagccggaac
     7861 agctcttccg cctcctcccg ggcaagggca agaggcttct cgcacaatac atgcttcccc
     7921 cgcaacaggg ccctcctggc catttcataa tggagatgat gggggacggc aatataaacg
     7981 gcatgaaccc ggtccagaaa ctgttcgtat tccgtataat attccagcag agcgtggaac
     8041 tctgcaaaac ggcggacttt ctcctcattc cttccgaaaa cggctgttat ttcaatattg
     8101 ctgacatatt tggactcccg gagaaaacgg cccgctatgc gcccgtgacc tgcaattccc
     8161 atatatacaa tgggattccg ggcggaacgg aggtctgttg aagaaacgcc cttggtgcgt
     8221 tccaggtaaa ccacctcaca ataatcgcga agatagtcaa atttccccga ccagtcggag
     8281 ccaatcacaa aaacatccgc cccgtacttc cgtatgtcat gaatcttctg cccttctagt
     8341 tcttccttaa taatgagatc cgccagaccg gttttccgca cattttccat gcgttcctcc
     8401 aggctctcca tgacattcag cttgccgcgg ctctggtcgt aactgtccgt agtcacgccg
     8461 acgatgagcc ggtcccccag tttccgggct ctcttcagca gattcacgtg ccccgtgtgc
     8521 agcaaatcaa aggttccgta agtaataacc gttttcatgg catgagcgca tcaatccgct
     8581 ttccgtaaac gtcaatgaaa cggttgaccg ccgcctcaaa gggaaccttc tccaccatgg
     8641 ccttcacgga aggatgcttg aactggatgc gttccggata agacagatag tcgccgtaaa
     8701 aaaattgcag gtactggcgg gcatggttgg gcacggaaaa tattatcccc tcaaattctg
     8761 cggagccaag gggaaagaac gtttcatagg aaaggtgcgt gttttttgta aacgtaatgg
     8821 caggagaaag aaaaatcccg ggggcttcat cttccccggc agcatccctt ccttccagaa
     8881 tatcctggcg gattttctcc tgaacctgcc catcggtcag gttcatcctg gaacctacca
     8941 gcaccacgtc tttcttaaat ccgctcaaaa gcctgtccag cttttcatgc tgttccggag
     9001 atgaaagact ctctgcataa aaatgatagg gatacacatc aaggttgagg ggggtgccct
     9061 catatcctat ttgcagaaag gcgtgcctgt tccaggtaaa tccttcctca cgggggaaaa
     9121 gaacgggaag cagttccagc aggcggtcga actccggcct catcatgctc acatccagat
     9181 catcatccca cggaataaag cccctgtgcc ttaccgcccc cagcaaagtg ccgtaatcca
     9241 gccagtaccg cagtccgttt tcccggcatt tgcgcgcaaa aagggccagc aaaaccgtat
     9301 tgccgtcctg aaggagcctc aacttccccg tcgccggggg aactgaggaa atagggtggt
     9361 tataaataag caaattgcga atatcctcca gcttcgcttc aagagaccgg tctttccttc
     9421 tcctgcattt cttcgcaatc cgtccggcga tttcccgcaa acggtaggga agattgcaaa
     9481 gcaccatata ttttatatgg cttattttac tcatatcctg ttagttgaaa aattaatccg
     9541 gttccaagga aacggcaaag catgattatt aaatgcggaa gaatcgtagc acatacattg
     9601 atctatacaa tgcttaatta ccccgtgctc agcagaaaga accactatgg catcgcgcct
     9661 cccctgaaga atatttccca gcgcaaaata aggaaaaaga cttattcctc cgtcacgcgc
     9721 ggttctttaa agcaatgaaa aaattccgga tggccatccc ttcacggaaa gccgcattaa
     9781 aacataacct tgtcccgtgc ggagcggcca taggccgtat cgggatattt atccgcagct
     9841 tcctcccaat acttccgggc cctgtccggg ttattgagct gggactcgta aatggctcct
     9901 atcttgtaca ggaacagagc ccggtcctcc ttgcgccacc ctccgtattc cagtcctttc
     9961 tgaaggacgt tgacggaatc cgcgtaccgg cgcatatcgt tcagcaggct ggattccatg
    10021 agccaggaac gcaccagccc cggattctcc ttccggtagc gttccagcaa ttccagagcg
    10081 ccctggggat ccccggaatt cagcatgcgc ctggaagcct ccaccagcac ttcctcatcc
    10141 gtagtaatat tggaggaata cagcgtcttg gtcaccagct ctcccatgac gggcagcaga
    10201 tgcctgatga cgagccacac ttcaaaggca atcaccggaa ccagcaccag cagggattgc
    10261 agaggggaaa ggggaatggc ctgcgccagg aacataaaca ggcccagcgg aagcagcacc
    10321 agcagcagcc gtataaacat gcgccgggcc agcagaaaaa tcattttcaa catggcaggg
    10381 gattcaagag aagccgggga gggggcatgc gcccccggaa agggggaacg cttccgcggc
    10441 ccttcccggt gaaagggccg cggagcagcc gcttaaaaac ggtaaacggc ttccacgccc
    10501 acacggatgc ggaagtcctc aatcgcgttc atgccggcgt agcggcggat ttcggaacgg
    10561 gtgtttcccg cccagtcaag ctggatgaaa ggaataatcc ggaacccgcg gctggagtcc
    10621 acacccgtta cgcgcacggg agccgccagt ttgatgttaa cctggtcaaa cccggctgcg
    10681 ccgggccccc agtaactgga ggaataacct gttccggcgc tcagctccaa atccaccttt
    10741 tcatgaatca ggccgctcca tcgcttgccc gcgttcaggt caatacgcca gccggtcatg
    10801 ccgtaaaagc cgtactgcac atccagggag ccaaaatagc cgtggcccgg atcgtcatac
    10861 gccagccccg ccgtgacgga ctgggcgagg gaatggggag ccttccccat gcgcttgctc
    10921 aaataccccg ggaggccgcc gtaattcaac tcatacccgc cccggaagga aagattgggg
    10981 aacagctctt tgacagcctt ccagctggcg tcaaaggcgt ccttgtcgct cagcagcccg
    11041 tcgcagaaag ccatgtattc cgcgccgatt tcctgcttcc agtctctggc caggttgagg
    11101 ttcagctcgc cgccgatgtt aaacacgccg ctgcggttca gcagggagga agccaccacg
    11161 tctttatagg cataccggga atcatagccg atatgggccg ctccgctgat ggtattgtta
    11221 taggaactcg cgggccgggg gtcataaccg tatccgtcat aggcgacggc ggaaaccgcg
    11281 gccagggcgg aagccgccag gaacnnnnnn nnnnttcata gtaagcttat cttaaccatt
    11341 tcccgcccgc caacgcaaga agaatcccgg cacggcgcgg agaaatggac agagaccctc
    11401 cgggaaaaat accatgaaaa aaagacaggg tctttcttga aaaaaaaccc ctttttccct
    11461 atgatggaga ggtaatggac tggaacgctg aatggtacga aaacaaacac ggttttgtcg
    11521 cggaatacgg cagggaactg ctctcctgcg taccggaaaa tccagaccag tccattctgg
    11581 acctgggctg cggaaccggt acgctgacgc acgc
//
LOCUS       DAWEMX010000080        11437 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2388_length_11437_cov_13.068617, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000080 DAWEMX010000000
VERSION     DAWEMX010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_11437_cov_13.068617"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3088..3097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3848..3857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2272 a         2814 c         3587 g         2744 t
ORIGIN      
        1 gactgaaaaa tatccagctt tccgccaggg aaaggtggga attttccctt tcccttgttt
       61 tccgaagttt gcggaatggt tttcaattaa atgttatttt caatgagagc ggtttgagcg
      121 agttttaaca ttcggaaaaa atcagagagt cggaatgaaa aggggaaatg atagaaatag
      181 gagccgatgg aagtttcttg ccaattttcg cggggtacat ggtaagagga aagttgggaa
      241 gacaggtatt gcaaaagaat gaaaaacgtg catattggag cccggtgaat ctgaattgtc
      301 cacagtgcaa acgacatatc aatgtgcctt cttccgcgga gggctctatg gtgaattgca
      361 gcaaatgcgg gcatgagttt ctttgcgaca gggaaaaact gattcctaag aaaccttttg
      421 ccaaagcgaa gaaagccgtt tccgtccatt cccccaaaga caggagggaa gccattcaga
      481 gcgctctggt ggaaacgggg ctgatccagt tggtgtgcct ttgcgcgtcc ctggctgttg
      541 ccgccggctg cgtctggatt ttttacaatg cttcttccga gatggatggg acctttttcc
      601 tttccatgtt gcagcagccc cagcagaagg cgtttgccct gttttttgcc tgtcttgcag
      661 gaggcttgat ggcctgtgcc ggacgcaggc acaagattgt tttcattttg ctgggtttgc
      721 tggttgcagg ggggattgct tccctgcctt tcgtgtatcc ggtcaaggtg aatccctccc
      781 tgctcggggg gcatgaagag gggagcgccg aggaagaaga tgtctccggc tttaccagca
      841 tggggctgga gcagctgccc tccaagaacg cttctgtcca gaattatggg gccggtgatt
      901 tgaaacctct gttttccgct attgaccgca aggaatccca gggggttctg ggcgtatggg
      961 tggttggggt gaatgcaacc aaccgggata tggtaaaggc atatttgaag aggatgaccc
     1021 agtccgagga cgaacctatt ttttatgacc gcaagggaac gggcggcggg ctgttcgtta
     1081 ttactcccac tcccattacg tttaaggaat ttgtagaggt tgcgggcaag atggggaagg
     1141 tgaccctgca ggataaggag cgtttttttg tggaagtgat tctgaaccgg gacaggtttg
     1201 aagcgcggcc cgcttccgcc gcgttgcagg atgagcgcca ccagtatttc gtgctggcca
     1261 atttgaagga gctgtcctgt ctggacatcc gccgcgttat tgccgccgca aaacgtttgt
     1321 cggctgtaaa tccggataag atgcggggtg aggtttcttc caggctggtg gagcttctcc
     1381 gggagccgtg ggggcgtgat gcggaatatg tgaccgcgtt ggcttcggcc cttgtggtat
     1441 gggcagaacc ggatcatgag gaggctcagc gcgtggttga ttacgtggct acggagctga
     1501 agaaggcgga ttgcgaaatc ccggcgtctc tgatcaggtt tttactgcat ggttcggaaa
     1561 agagaagttt ggcgttgctt ctggaggaat ggaaaaagga tccccagaag tgggaggacg
     1621 aatgcaaggc tgccggccca acgggagaag ctgatatcat ccgttcgctg aatgagtcgg
     1681 acgattttgt gttgaaacgt tccgccgccc gaattcttgg ggaaatcggt tcggaagctt
     1741 ccctgtccgc gcttaaggct ttcactcatg acgccgataa cgaattgcgc ctgtgcgcgg
     1801 agttgtctgt gaacctgatt gaaaagcggc ttgggataga ttcttccgcc aaaggaggcc
     1861 ggtaagggcg gcttccgttt ccgtgatggc gcatcgcgct gtttttctgg cttcaatacg
     1921 gaaggggagt tttttccaga cggtttacgg ggattgttac ggtaattgaa gtgcggctcc
     1981 ggaattttcc catggctgtt tttcggagcc gggggatgca tgagggcatt tctgcagagg
     2041 tgtccaaatg attttctgtt cccggacagt ttgttacttg tttcttttac gatattttca
     2101 ccatttgaac gcatttccgg ggaggaaatt tgtttttgcc cgggaagcgt ctttttttgg
     2161 ttgcggagaa gagccaattt actaaaatgc aacgaccatg acagaccaag aaaacatgaa
     2221 agccgccgtt ccggatgcgg ccaggcgata tatgcgctat ctcctcatca tggccggttt
     2281 gggcggcctg ctctacggtg tggacgttgg cgtgattgct gccgcgcttc cctacattga
     2341 gcagacggcc ggttttaatc cctcccagct ttcccaggtg gtggccgccg tgctgttcgg
     2401 cagcgttctt tcttccctgt ttgcgggcta tctggccgac aagatgggac gcaaggcgtt
     2461 gattaccgtg gccgcagcct tattcacggc gagcatcccc gtgatctgcc tgtcccagga
     2521 agtattcggc attatgttgc tgggccgtat tcttcagggc gccagcgccg gtattgtggg
     2581 cgtggtcgtt ccgctgtatc tggccgagtg cctgagcgcc gagtcccgcg gcaagggaac
     2641 ggggatgttc cagttcctgc tgacggtggg cctggtgttt gccgccgtcg tcggtttgct
     2701 tgccgccagt tatgtgggcg gcgtcgagaa ttccggcgcg agcgaagaat cgctgacttc
     2761 cgccaaggtt ctggcctggc aggctatttt ctgggtttgc gccattcccg gcctgttcct
     2821 gtttttcggt tccttccgtt tgagcgaatc tccccgctac ctgttccgcc gcggccgcaa
     2881 ggatgaagcc atggctgtgc tggtgcgcag ttacggggat gcccgcgcca aggaagtgtt
     2941 tgatgaaatg atccacattg aagaggaaga gaagcagaag gcggaggaac tcaaaaagca
     3001 gagctcttcc ggcgagtccc ttcttcagcg caagtatatc tatccctttg ttctggcggt
     3061 gctcgttctg gcgtttacgc aggctacnnn nnnnnnnggc tacgggcatt aattccgtgc
     3121 tgaattattc cgtgaaggta ttccagcagg caggcctgga aggcaccacc gccaactggg
     3181 cagactttac catcaaggtc gtgaactgct tgatgactat tgtcgccatg gtgctggtgg
     3241 accgcaaggg ccgcaagttc ctgcttaaaa tcggaacggc cggcatcgtg gtcggccttc
     3301 tcggcaccgg gttcctgttc aataatgtgg aaaaagcccg caaggatgtg actgcggatg
     3361 tggctgccct gctggccgcc cagagccctt ccgtccagaa ggagtttgaa cagggcaagg
     3421 atgtgggttc catccgaacg cttcaattgg aacgcactcc ggattccccc ttcatcagga
     3481 atcttcttgc caagaacggc atggccgaca aggacatcaa caggatgcag ctcatcatca
     3541 cctatgacca gccggaagcc aatcccgcct ggtaccagtt cctgatgggg tcttccaccc
     3601 agctttccgt cgtagagttt tccgaactga ccaaggatac caaggatatc aagaaggaag
     3661 aggacagggc ttccctggcc gtgatcaagg ccgttccgga ttccaccaat aaaatggtgg
     3721 tgaacggcaa ggacggctat gccatgaagc ccgtttccat tctgaaggcg gaattgggcg
     3781 aaaagccgga tacctccatg ggctggggcg tgactgcgtt cttcattatc ttcattgctt
     3841 tttatgcnnn nnnnnnnttt ttatgccacc ggccccggcg tgtgcgtctg gctggcgctg
     3901 tccgagctga tgccagcccg catccgctcc aacggtatgg cgattgctct gttgatcaac
     3961 cagctggttt ccacggttat cgccggttcc ttcctcccat gggtgggcag ctgcggttat
     4021 tccggcgtgt tctttacgct gggcggcatt acggtgctgt atttcattac ggtgaccttc
     4081 ttcctgcctg aaaccaaggg acgttccctg gaggaaattg aaggttactt cacaacaggc
     4141 aagatgccgg aagatcccaa gatgatcggc gaaggcatag aagcggagga ataattcttt
     4201 cttcctgtgg ttttcaaggc cgcttcccct gaattgggga agcggctttt ttataacagg
     4261 aaacagggag ggctgcctgc atggcttcgg aagcacggct gttccgggga gagggttccg
     4321 gccgcctgtt cccgggccgt gacggtatcc gcttttcttt cccgcaccag tgggagaagg
     4381 agggctgcaa agcctgcgat ggcgtccgct atttagcggg cttccccttt tttctgcttg
     4441 gactgccagt gcttgatggc ttccgcaatg gggggatacc cgtttttcat atggaagtca
     4501 taggctgtct ggccgtcatc gttgcggaga gagggattgg cgccgtgatt catgaggcaa
     4561 acggctattt ccggagagtt gcaggccatc agcggagtca ggccatccac gtccggctgg
     4621 ttgggatccg ctcccgcgtt gatgagaagt tcaatgagag gaatgaggcg ttccgccggt
     4681 ttttccttgg tgcagaccag gtggaggggt gattgcttgc tttcaaaaaa gcgttcgtta
     4741 acatcgcagc cggctttgat gagccgttcg taaatggcgg gggacagact gaggaagccg
     4801 actccgcgcg cctgagcgtt cattctgcgc ttgagatccg gggagccggc gtccaggagc
     4861 gccagcgcta ttttttcctg acccggatcg gagtcattga gggcggcgga atacatgaac
     4921 ggggtgacgc cccaaggccc cggctgatcc ggatcggcgc cgtgtttgag cagggcgtgc
     4981 accagcggca gcctttcttc cgcggtggaa ttcatataga ggcctgagag gcacgcccag
     5041 gtaagggggg tgtctcccgg ctcttttgtg ccgtcatgca taaaaatgga ttgagcattg
     5101 acgggagcgc ccgcctctac catccggacg gccagccggt ttaatcccag ggcaatgcag
     5161 aggtgcagag gcgtggtacg gtccggcgtc tggagcagga ggttgccggt ttggcggaat
     5221 tgctccagca tctgatggca ttgaataatg gagtggaatt cctcctgcac ttctccggga
     5281 aacatgattt ccagcggcca ggagtcatgc aggaaacgga gggtaaggcc cagcagcttt
     5341 tcgtttgccg tcttgatttc ctgttgttcc gcttccgtga gctggattgt cttttcctcc
     5401 gcatgcgccc agggagcgac cataacgact gctgaggaca gaagcagcat ggttcctgtc
     5461 atttgtctca tgaagtcctt ataggggaag ggccgtgagc tgtcacttca aatcaggggc
     5521 atgtcaaccc agcctggaga gactgatgcg gacggcttcc accgtgcggt caatatcttc
     5581 ttccgtatgg gctgtagaga taaagcccgt ttcgtacggg gaaggcgcga agtagacgcc
     5641 ttgttccagc atgttccaga agaaggattt gaagagtttt ttggaagctt tttgcgcgga
     5701 ttccagatta tagacttcct cttccgtgaa gaagaggcag aacatggagc ccgcacggtg
     5761 gaaggtgtaa tttctgccgt gttttttgag ggcttcgcgg atgccttctt ccatgcgcgc
     5821 tccgagctgt tccaggcgtt cataggcgtt ccctttcagg agttcccgga gctgggccaa
     5881 tcccgccgcc atggcaacgg gattgccgga cagggttccc gcctgataca cggggccaag
     5941 cggagagagg cagtccatga tttccgaacg gccgccaaaa gctcccacgg ggaggcctcc
     6001 gccgatgact ttgcccatgc aggtaaggtc tggagtgatg ccgtaaagct gctgcacgcc
     6061 tccgggagcg acccggaagc ccgtcatgac ttcatcaaag atgagaaggg cgtcgtggcg
     6121 gagcgttatt tcccgcagga agtgaaggaa gtcgttttgc ggaaagtaga gtccggcatt
     6181 ggcaggaaag ggttccagga tgacggcggc aatgtcgctt ccatggagtt caaaggcttt
     6241 ttttactgct tcccggtcat tgtagggcac ggtgagggtg agctgcgtca tttccctggg
     6301 cacgcccgcg ctgtccggtt ctccgaaggt gagcgctccg gaacctgccg ccaccagaag
     6361 ggagtccaca tggccgtggt agcagccgat gaatttaatg attttacggc gtccggtata
     6421 gccgcgagcc agcctgatgg cggacatggt tgcttccgtt ccggagttga ccatgcgcac
     6481 tttttccacg gagggaacca tgtccgtgat catttccgcc atatccactt ccgccggcgc
     6541 aggaattcca tagcccagcc ccctgtccac ggcggcatgt actgcgtcca gaacgggttg
     6601 cggagcgtgg cccagaatgg ccgggcccca tgtgcctatg tagtcaataa gctggcgccc
     6661 gtccgcatcc gtgatgtagg ctcctttggc ggattgaaca aagaaggggt ccccgtccac
     6721 attgcggaag gccctgacgg gggagttgac tccgccgggg ataacggagc gtgcgcggga
     6781 gaagagctgg gctgactgat tcatgtgcag agggaagcaa aaagggcaat ccttgagaaa
     6841 gggttgccct catggaaatt gggttcttat tcccgaacta atactgtttt aagcttcagg
     6901 agcggaagcg ggtgtttcag cagtttcgga agcggtttct tcgctcttgt tgctgtaacg
     6961 ccattttacc ttgttgcgct tgctggcgga acgggccttg cggcggcgtt cgtccatcgg
     7021 ggtttcaaag gcgcggcggc ggcgcatttc atccagaatg ccttcaacat ccagttttgt
     7081 tttgaggcgc ttgagagcgc ggtcgatagg ttcacctttt cttacggtaa cttcacgcat
     7141 gatttgtttt cctttttcta ttcgtccggg aacgggagac aataggtagt gctttcctgc
     7201 ttacaggtca agatttttat catgactgac ctattttttg ccactgaatg agagttctgc
     7261 cgtccggaac ggcgggaagc ggccgcgttg ccccgttttc cggttccgga gcgcggttca
     7321 actgtccttg ccttataaca gggggcgtgg tagaaagagg cgtctcccat gaaccggctg
     7381 catacattga ttgacggatt ttcccgggtg cggattctct gcatcgggga cgtgatgctg
     7441 gataagttcc tgtatgggag cgtcagccgt atttcgccgg aggctccggt tcccatcatg
     7501 aagatggacc gggagacgcg catgctggga ggcgcgggca atgtcgtgtc caatctgtgc
     7561 gcgctgggat gccgcaccac ttttatcagc gtggtcgggg atgatggcca tgggcggcag
     7621 gtaaggcgtt ttctggaaga gacgcactgc gttccggagc tggtggagtg cgaagggtac
     7681 gagacgaccg tgaagatccg ttttgtggcg ggcaagcacc atttgctgag agcggaccag
     7741 gaacagccgc tgaggatgga gccggagctg gcttcccgtt tttttgaacg agtggatgcc
     7801 tgtttgccgg aggcggatct tgtccttttg tccgattacg ggaaaggcct gtttgacgga
     7861 cgaaccgccc cggatgtgat tgcccggtgc cgtgcggcgc gcaagcccgt cattgtggac
     7921 ccgaagggag cggattattc ccgctacagg ggggccacgc tggtgaaacc caatatgaag
     7981 gagttccagg aggctaccgg cgtggcgctc aatcctgcca tggccgggtg ggaacaggat
     8041 gccgtggcgg gggcgcagag gctgttttcc gaattcggca ttgagaatct gattgtcact
     8101 ttgagcgaac acggcatgat tttcattcct tcctccagcc cttccgattt tgtctgcatt
     8161 cctacggagg cccgggatgt ttttgacgtg tccggcgcgg gtgatacctc cctcgccagt
     8221 ctgggcgcgg cgctggccgc cggagccgcg gtgccggatg ctctggtcgt gtccaatgtg
     8281 gccgccggca tcgtagtggg caagttcgga acggccagcg tgaccggaac ggaattgaag
     8341 gaggcgttga aggtgaagga tatccagacg ccctcctggc atcaccgcga caatatcctg
     8401 acgccggaga ccgcggcgga gctggcggaa cgtttccgcc gtgagaagaa ggtggttgga
     8461 tttacaaacg gttgttttga tttgctccac ctggggcatc tgcattcctt tatgaaggcg
     8521 cgtgaagcgt gcgacgtgct tttcgtaggg ttgaatacgg acgcctccat caagcggctg
     8581 aagggcgagg acaggccgat taataatgag gagatgcgtt ccctgttgct ggcatccctg
     8641 gattttgtcg attatgtagt gccgtttgat gaagatacgg ctctgcctct gattgagaag
     8701 ctgcgtccgg acgtgattgc caaagaaggg tatcccctgg aatgctggcc ggaaggacag
     8761 ttcgtgaaat cttatggagg gcaggctctt gaactgcccc gcctggaggg tttttcctcc
     8821 accaatatca tcaaccgcat gaaaagcagc ccggaatgag cgaatacatc aggaaccaga
     8881 ttttagggat agcggacaat ttcaaagccc tggcttccat ggctggggat attgagcagg
     8941 ttgcccgcat ttgtacggat acccttaagg ccgggaataa gatcatgttc tgcggcaacg
     9001 gaggctccgc tgcggattcc cagcacctgg ccgccgaatt ggtggggcgt tacaagctga
     9061 accgccccgc catgaatgcg ctggccctga cggtggatac ttccatcctg accgccgtgg
     9121 gcaatgatta cggttatgag acggtgtttt ccaggcagct ggagggagtg ggccgtcccg
     9181 gagatctgct ggtggggctt tccacgagcg gcaacagccg gaacatcgtt ttggcgatgg
     9241 agctggcgcg ccggatgggc gtccggacgg tggccttgac aggccgcgga ggcggagaga
     9301 tgaaagaagt tgcggagttt tgcattgccg ttccttccga tgccacgaac aacattcagg
     9361 agatgcacat tgcagtgggg catctggttt gcgaactggt tgaacgggag atatatggcg
     9421 ggtaaggctt tgtttctgga cagggacggc gtggttaatg tggatggcgg gtatgtacac
     9481 cgcatagagg acttccggct ggttcccgga attctgaatc tttgccgcca ggctaaagag
     9541 aaggggtatc tggtgctggt ggcgaccaac cagtccggca taggacgcgg catgttttct
     9601 gaagatgact ttgagcggtt gacggaatat atgagagggg tgttccgttc gtccggtgcg
     9661 gagattgcag gtgtgtttca ctgtcccagc gcggatgacg gacatcccga ccggaaaccg
     9721 aatcccggcc tgtttttgaa ggctgccgcc gcgcatgggc tggatatgaa agcctgcgtg
     9781 tccgtaggtg accgggagcg ggacatccgg gcggctcttg ccgccggagt aggccgcaat
     9841 ttcctgtttt ctgcggaaga tgttcccacc ctcgccacgg cccgtgtgga gacgttggag
     9901 gaagtggcgg caaggctgta ggccgggaat agttgcttgg ggaaaatcgc tgcggtttgt
     9961 ccctggagtt atcccctgca gcggggcggg cgacatggta ccggtgcggg gcaaagtatt
    10021 ttgacatacg gagaggagcc tgttagaagt ggaacaatcc ggccgtagtc tcgtgaacct
    10081 ggcctgtaca ggctttttat ttttgcacgt atgaatctga tacttttcca atccatgatg
    10141 gctgccgccg cgctgggggc cggccttgcc cttcctgttt ctgcccagtc ttcttctccg
    10201 gctttttccg cctctcctga aggctcccgt gccggaaaaa cggccaagat gccggtgtat
    10261 cccattccgc agaagatgac caggagcggc ggttccgtga cggttccggc cctgaagctg
    10321 ttgggcgcgc gggatgaacc taccgtgaat gccctgaaaa gcatgttttc cctcgctcca
    10381 aacgggctgc ctgtgcagat gactatcatc aaaggaagca aaaagcctgc cgataatgtt
    10441 ccccggaagg ccggggccta ttccctgagc gttacgcccc aggggatacg gatgacgggg
    10501 tatgacgacg aaggcctgtt ttacgcggcc cagacgctgt tgcagttggc ggagaagact
    10561 tcttccggcg tgacagtgcc ggaagtggag gtcttggatt ggccggatgt tcctttccgc
    10621 ggtaccattg aagggtttta cggtctgccc tgggggcagg aaggtcgcat cagccagttc
    10681 aaattttacg ggaagtataa gatgaatact tacatctacg gcccgaagga tgacgtgttc
    10741 cacggttttt ccaagcgctg gcgcgagcct tatcccgcag atatggcgaa ggacctgaaa
    10801 gagctggtga aggtcgcgaa gcagaataag gtgaattttg tctgggccgt gcatcccgga
    10861 gcagatattc actggggcga ggcggaccgg aaggcggccg tgaagaagtt tgagatgatg
    10921 tatgatctgg gattccgttc ctttgccgta ttttttgacg acatcggcgg agagggggcc
    10981 aaaccggagg gccaggtgga gtttttgaat tacctgaata aggagtttat ccacaaaaaa
    11041 ccggatgtga cgccgttgat cgtatgcccc acggcgtatt ccggcggtgg tagccgctac
    11101 catgaggtaa tgggggaaca tctggacaag gatataggca tcatgtggac cggttccagc
    11161 attgtgagcg atatccggac tcctgctttg aaggggatta ataagtattt gaagcgtccg
    11221 gcgtttatct ggtggaattt cccggtgacg gattacgtgc gccatgccct gtttttgggg
    11281 cgcacctatg gcgtggatgc ggacgcgatg ccgtttatgc aggggtttgc ttccaatccg
    11341 atggataagc cggaggcttc caagatttcc ctgttcagcg tggcgaacat gacttggaac
    11401 gccaaggcct atgattccga caggacatgg aaggaca
//
LOCUS       DAWEMX010000081        11429 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2392_length_11429_cov_10.320204, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000081 DAWEMX010000000
VERSION     DAWEMX010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2392_length_11429_cov_10.320204"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1567..1576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9085..9101
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9388..9397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2528 a         2743 c         3424 g         2697 t
ORIGIN      
        1 atggcgccgc ataattacca gggagccgtc cgggatgagg ggttccatgg atttcccttc
       61 tacgcggacc acgtattcgt ttttacccag gggcctttcc atgtaaatgg tagacgggac
      121 ggtgtcgctt tccgcaagag agcctgcggc gatgttgccg attatttgcg tactgtattc
      181 acgcctggcg tgtccagcat gggacggaaa ttgtctgacc aggggaacca ggggggagtg
      241 cattggccgc agctggcgtt cctccgccgc ttcgtctgcc aaatccgtca gccactggcg
      301 gagggggacg cctttttcag aggcggcccg ttcatatttc ctcatgcgct cttccgtgat
      361 ttccagcacg aggcgattgg tctggacagg ttcgtcttcc ggtcgaaccg ttttgtattg
      421 agacatcagg gattggattt tcaacatggc gctggcgggg aaggggccgg agggggagag
      481 ccagttattc accgtacctt tttccgtacc gcattccatg gcaagccagt cacggttttt
      541 cccaatggcc ttcaaccagt gtttcacatc cccttttgtc ggtgtcatgg cttgattata
      601 cacgattttc gtgaattgtc gatagaaaga aataaattga attcgttaaa atgtgttgac
      661 gtattaacga aaatcgttaa aaaggcgcta tgaagtacat gcctttagac ctttccgtaa
      721 tgcagccgga gccgtttccg catcccgtga tgatgaggtc cgacctgtat cccagaagac
      781 agccctcccg ggaagaaaag tggggcattt gccccggttc cgtctgcggc gcgctgcggg
      841 cgctggttcg tcgatgctgg gaagaggcgg agcggcggaa gctggcaatg aacattcttt
      901 ccgtcgccgg agcctgcgtt ttcggactga tggctgtcgg cgtgttctgg ctggctgttg
      961 agatggataa ttccgctttg caggcttcca agtcttcctg ttccataaaa ccgtaagccg
     1021 gcccttgtcc aaggggggcg gaacgctttt ttcctgcgga gcttgctttc ggaaatgaac
     1081 ggccgcgttt gatgcctccc ggtttgtttt ttccggttga aacgcctgcg gacgcatgaa
     1141 aggttccggc aggaatgggc gccggatttc cagaggggct tttttatccg cagagcaatt
     1201 tgaacgggag agacggggaa gggctggagt ttggatgact gcattatttg ccggctgcat
     1261 cacccgggtt ccggaaatgc cgtccgccaa tgccgttcct gtgcgggaat agcggttggt
     1321 cttgcaggcg ggcagtatgg cgcccgtttg cgaagaggat gaagctgcgg ttccggattt
     1381 ctgtccggag ggaaccgcat gagatggagc agggatgctt ttcctccacg tggccgtttc
     1441 ccgtttatct ggcgcggttt gatttcattc tggcgtggcc gccgggaccg gctgttgaag
     1501 gagtttcctg aggcgcaagc gggcacaggc ctgtatggaa atcccctgtg gtgtctgtcg
     1561 tgccggnnnn nnnnnnaggc ggggatgctg cgacctgggt ccggggcgcc catcattccg
     1621 ggcgttatgg cgctggtcgg gagactggcg gcggaagatg ccgccaggaa cggcggcctt
     1681 ccgcttctgg attcattccg ggtaaaagat gcgccttttg cggagaaagt gaaaaatgaa
     1741 gaggcttttt ccgattttgc atgttgagaa ataattttat gacagttgat atgtattcat
     1801 tataaatgaa ttaaaaataa tgaagcagaa agtgctaaaa atcttttgca ttatagttac
     1861 tataaggaat agcctgaatg aagaaagaag aaggcatggt acgcggccgg cgtcctttcc
     1921 cttctttttc cggaatccgg ccatcgccgt ttaaaaggca caccatggaa aacaacagac
     1981 tttttatcaa atcatcacca tgaccaagag aatgattaaa aatgcttcct acgaagggga
     2041 acgccccctg ttcgcttccc gccatcttag gctggaagac gtcattattt atccggggga
     2101 atccgccctg aaggaatgtt ccgacattga agctgtgcgg tgcgagttcc gggggaagta
     2161 ccctttctgg cacaatgacg gactgctggt ggaacattgc ctgttcaggg aaggcgcccg
     2221 ggccgccatc tggtattccc ggaatctgca catgaaggat acccgcgtgg aagcgcccaa
     2281 gatgttccgt gatatggacg ggatggttct ggagaacgtc gttctgacgg atgctctgga
     2341 atgctgctgg cactgccgta atgcgaagct tcgcggcgtg caggctgagc atggggatta
     2401 cctgttcatg cacggggaga atatagatgt tgacggcttc cgcctgaacg gaaattactc
     2461 tttccaatat tgcaggaatg tggtgatacg gaatgcggaa atccattcca aggacgcttt
     2521 ctggaatacg gaggacgtga cggtgtacga ttccgtggtg gacggggaat atctgggctg
     2581 gcattccagg aaccttcgtc tggtgaattg ccgcatttcc ggcacgcagg cgttgtgcta
     2641 cgcggaaaac ctggttctgg agaattgcac gctcggagag gatgcggatc tctgctttga
     2701 gtattcctcc gtgcatgcgg aaatcaaggg gcgtgtgcac agcgtcaaaa atccgcgcag
     2761 tggccgcatt gttgcggaag agtacgggga tattattctg gacgagcatt gcaaggctcc
     2821 tgccaattgc tccattgaaa cgggaaaagc ccaatccgga gaagccgcat gaagtacgat
     2881 tttgatactg ttgttccgcg ccgcggcacg aattcctaca agtgggattc catgccccgg
     2941 gaggatattc tgcccatgtg ggtggcagac atggatttcc gtacggctcc ggcggtgacg
     3001 gacgccatcc ggaagagggc cgagcacggc attttcggtt atacacgggt tccggattcc
     3061 tattatgagg cggttacaaa ctggtttgaa cgccgccacg gctggaagat taatccggaa
     3121 tggatgattt ataccacggg ggtggttccc gcgctgtctg ccgtcatacg ggccctgacg
     3181 gtccctgggg acaaggtgct ggtgcagacg cctgtatata attgcttttt ttcctccatc
     3241 cgcaataacg gatgcgtggc ggaaacctgc cctctgaaat atgagaacgg cagatatgcg
     3301 ctggatgcgg aggagctgga acggaaagcc tccgatcccg ccgtcaagct gatgctcctg
     3361 tgcaatcccc ataatccagc cgggcgcgtc tggacgaggg aagaattgga ggagatggcc
     3421 gccatttgcc ggagaaacgg cgttttcata gtggctgacg agattcattg cgaactgacg
     3481 tatccgggcc atccctatac gccgttcgcc tccctctccg aggacgccct gcaccattcc
     3541 gtcacctgta tttcccccag caaggcgttt aatctggcgg gcattcagat agccaatatc
     3601 gtcgcggcgg atgaagaagt ccggaagagg attgacaagg ctatcaacat caatgaggtg
     3661 tgtgacgtca attcttttgc cgtagatgcc ctggaagcgg cttataacgg aggggaggac
     3721 tggttggagg aactgaagct ctacctttac gggaattacg agattgtccg ggatatgctg
     3781 gcagaacgtc tgccgcagct ccgtgtggtg ccgctggagg gaacgtacct ggtctggata
     3841 gattgttccg ctctggggct tccctccgca gagattgtcc gcctgctgga agaggagggt
     3901 cgggtgctcg tcaacggcgg ggaaatgtac ggggaaacgg cggggtgctt cattcgcctg
     3961 aacgccgcct gtcccaggaa gctgctgctt gaggggctgg aaagaatcat ccgcacgctg
     4021 tgcggccgca tttccggaac acggacgtgc ccgtcgggcc ctgcaggctc ttaacaggga
     4081 gaacggttat taatcatcaa caatcaagtt gccatggaaa ataagatgac gagaagaaca
     4141 tggctccagt cttccaccgc agccctggca tgcctggctt tgccggagta tgccctgggt
     4201 gcggttgcgg agaagggtgg agcttccagg gtctggatga cgaaggaaat ttctccggag
     4261 gctctcgtga ggatttacga ggctctgggg cgtccggccg ggggaaaggt agctgtcaag
     4321 atcagcaccg gggaaccggg aggccgcaat tttctgagtc cggccttaat caaagacctg
     4381 gtgcgccggg tgaacggcac cattgtggaa tgcaatacgg cttacggagg caaacgctcc
     4441 cggacggagg accatttgca ggctgcgaag gatcacggtt tttccgatat tgcgcgggtg
     4501 gacatcatgg atgcggaagg ggagttcact atcccggtga gggacaggaa gcacctggaa
     4561 tacgatatcg tgggggatca tttaaagaat tatgatttca tggtcaatct ggcccatttc
     4621 aaagggcatg ccatgggcgg cttcggcggt gtgatcaaga accagtccat cggtgttgcc
     4681 tcggcagccg ggaaggcgta catccattcc gccggaaaga cgcgggatgt ttcctccgtg
     4741 tggaacaatc tggccagtca ggatgatttc ctggagtcca tggcggcttc cgcgcaggcg
     4801 gtggcggatt acttcgggga cagaattttg tacatcaatg tgatgaataa tctgtccatt
     4861 gactgtgatt gcgattccca cccccatgcg ccggaaatga aggacatcgg tattctggcc
     4921 tcccttgatc cggttgctct tgaccaggcc tgcctggatc tcgtttacgc cgtcaggccg
     4981 tccgaaggga atgacaacag gcccctggtg gcgcgtattg aaagccgcca tggacggcat
     5041 acggtagagt atgccgagaa gataggtctt ggcagcagga agtatgaact gaaagagctg
     5101 aaaccgcagc aggccgttta gtcggtcatt tcctttttag tatcaaaaac aatagaaaaa
     5161 actgtgatgg aaaaagtgat gttgaacaat ggcgttacga tgcctgtcct gggatttggc
     5221 gtttttcaga ttccggacgc taaggagtgc gaacgctgcg tgctggatgc gattgaggtc
     5281 ggctaccgtt ctattgatac cgcgcagatt tacggcaatg aggaagccgt aggccgcgca
     5341 gcggagaaga gcggggttcc gcgggaagag ttgttcctta ccacgaaggt gtggatttcc
     5401 aacggcgggt atgagaaggc gaaggcgtcc attgacgaat ccctgcgcag gctgcgcacg
     5461 gattatctgg atctgctgct catccaccag cctttcaacg attactacgg cacttaccgg
     5521 gccatggaag aagcgaaccg ggccggaaag gtacgcgcca ttggcgtgag caatttttat
     5581 ccggaccgtt ttgtggacct ggcggagttc tgcgagatca gacccgccgt caaccaggtg
     5641 gaaacgcatg tgttccacca gcaggaaaaa gtccgggagg tgatggaaaa gtacggtacc
     5701 agaatggaat cctgggggcc ttttgcggaa ggtcgcaatg ggtttttctc caaccccgtg
     5761 ttgaagggca ttggagagaa atacggcaag accccggcgc aaacagccct gaggttcctg
     5821 attcagcgtg gaattatcgc cattccgaag acgacccacc gggaaaggat ggaggagaat
     5881 ttcaacgtct ttgattttgc cctttcccag gaggatatgg gcgctattgc caggctggac
     5941 cgcggagaga gccttttctt gtgccaccgg gatccggaat cggtcctgta cctgatcaat
     6001 tacgggaagc agtaattcgc atgaagttcc agaaagatga cacgatactc atttaaaata
     6061 ttttgcgtgg ctttttccgc ctgcttttgc tgtgcgggga gcctgttggc agaagataat
     6121 aacccccata cggacaagat gatgaacgag aagctgaatt taacgcagga atgggacaaa
     6181 gtgtttccta aaagcgacaa ggtaacccac cgcaaggtaa gtttctgcaa ccgttatggc
     6241 attatgctgg ctgcggattt gtatatgccc cggaatgtta acgggaaatt gccggctatt
     6301 gccgtttccg gccctttcgg ggctgtaaag gaacaatctg cgggccttta tgcccagacg
     6361 atggccgaac gaggttttct gacgatcgcg tttgatccct cgtatacggg agaaagcgga
     6421 ggatttcccc gctatgtcgc gtctccggat atcaatacgg aggatttctg cgccgccgtc
     6481 gattaccttt ccacccgcga tgacgtggat tcggaacgta ttggaatcat tggcatttgc
     6541 ggctggggcg gcatggcggt caatgcggcg gctatcgaca cccgcatcaa ggcaacggta
     6601 acctccacga tgtacgatat gagccgcgtg aacgcgaacg gctatttcga cgcgatggat
     6661 gccgacgccc gttatgagct tcgcaaacaa ctgaatgccc agcggacggc tgatgcgaag
     6721 agcggttctt atgccctcgc ggggggcgtg cctgatcctc tgcctgcgga tgcccccgga
     6781 tttgtgaagg attattacga ttattataag acgccccgcg gctatcacag gcgttcgctc
     6841 aattcaaatg gcggatggaa tgtcacttcg gcgctttcct tcatcaatat gcccctgctg
     6901 gcgtacagcg gtgaaatccg tagcgccgtg ctcatgattc acggggaaaa agcccattcg
     6961 cgctatttca gcgaagacgc cttcaggaag ttgaaggggg acaacaagga gctgatgatt
     7021 attcccggtg caagccatgt ggatctttat gacaatcaag ccggtgtcat tcctttcgac
     7081 agaatcggag agttctttct ggagcatctg aaataaggag gctccgcatc tgaatttggg
     7141 aaagaaagac ggaacgtaat gaaacgcaag gattttttga agataacatc cggtttggcc
     7201 ttatccctgg tttcccgggg atgggccggg gtgggttctt ctttgctgtc tgacggtccg
     7261 gggtcttctt ccggggtgcc gaagaagggt tttcttgggg aatcccggcg ccttggaggc
     7321 ctggaagttt cccctatcgg gctgggatgc ctgccgatgg tgggttatta cggcggcaag
     7381 tatgataaac aggagatgat tgccctgata cgccgggctt ttgacaaagg agttactttt
     7441 tttgatacgg cggaagtgta cgggccttat accagtgagg aatgggtggg ggaggctctc
     7501 gcccctgtcc gcaaccaggt caggatagga accaaattcg gttttggcgt ggaggaaggc
     7561 cgtccttctt ccctgaacag caggcccgac catatccggc gtgcggtaga aggttccctc
     7621 aggcgtttgc gtaccgacca cattgacctg ctttaccagc accgggtgga cccggatgtt
     7681 ccgatggagg aggtggcagg tacggtgaag gaactgatgc aggagggaaa agtgctgcat
     7741 ttcggcctgt ccgaagccgg cgcccgttcc atcaggaggg ctcatgccga gtgtccggtg
     7801 agcgccgtcc agagcgaata cgctatctgg tggagggaac cggagacgaa gatttttccc
     7861 acgttggaag agttgggcat cggttttgtt ccgtattgtc cgctggggcg cgcctttctg
     7921 gcaggagccg tccgggagga cagccgtttt caaaagcggg accgccgcgc cactttgccc
     7981 cggtttactc cggaagccct cagattcaac atgccgctgg ctgttcttgt ccgggaatgg
     8041 gcggaacgca ggggcatgac tccggcccag ttcgccctgt cctggatgct ttcccggaaa
     8101 ccgtggattg cgcctgttcc cggaacaacc aatccagccc atctggatga ttttctggga
     8161 ggggcttccg tccgcttgtc cgaaccggaa ctcaaggaat tcgaccttgc ctgttccaga
     8221 attcccctga tggggcaccg ggcggatccg tttacggaga gccagattga caagtagttt
     8281 tgccggcgcg tttccttccg ctccggaaaa atgccgcggg actgtttgcg gcggaaggat
     8341 gttaagctat ttttaagaaa gagacatatg attctgattt tcggagcatt gctggcggtt
     8401 tacgtttttt gccgcgctat tttgccgctg aagctgaaat ggggctggaa gcttctgctt
     8461 gccgccctgc tggcggtggc ggcattcaaa ttccacttgc tgcatttgtt tggaggcccc
     8521 atgtttttct ccccggtttt gccggaaaac gttctgctgg cggccgcgtg gctgttctcc
     8581 gttcttttcc tgttcttttt cctgctgctg gcggcagatg tggtgcgggc tctgtatctg
     8641 ctggttttgt tctgcttgcg gaggaacagg acggaaaggt ttcgcatcat tggcaaccgg
     8701 gttaatgttg ctttgctggt tttttccgcc gtgctggcaa ctgtgggcat gattggaggc
     8761 accagagtgc cccaggtgaa ggaggaaacg gttgccgtga accgcctgcc ggaggaggcg
     8821 gacggattga cggtcgccgt gctggcggat ctgcatgtgg acggcattac aagggaggag
     8881 cgcattcgga agattgtgga gcgcacgaac gccctgaatc cggacatcgt cgtgattgcc
     8941 ggagatttcg tggacgggac ggtgcccgtg catggcggcg atttgaggcc gcttgcggat
     9001 ttgaaggcca gatacggggt gttcggcgtg ccgggcaatc atgagtatta ctccggttat
     9061 gaggagtgga tggagttcct gcccnnnnnn nnnnnnnnnn natccttgtt tcccaccagc
     9121 cgcggctggc ccgggaagcg gctgcgcacg gcgtggatct tcaagtgtcc ggccatacgc
     9181 acggaggcat gattgtcggc gtggaccggc tggtggcgcg ttttaacgag ggattcgtct
     9241 ccgggctgta cacggtgggt aatatgaagc tgtacgtttc caatggggcg gggatttgga
     9301 acggctttcc catccgcatc ggcgttccat cggaaattgt tctgattcgg cttcggaaag
     9361 aataacatat attgataaag cagtttannn nnnnnnnagt gatgcgggca ggattgccgg
     9421 aattgttgaa aaaatattga cttatagtta ctctaatgtt tagacagaaa taaggaacaa
     9481 ggtcaggcaa gggaaatgcc ggatgaaacc gttttttccg gacgcctttt gcctctcctt
     9541 ttaaccggat aaaaatacat gaaaacaata tcaatgatat gcacgctggc cgcggccctg
     9601 atgacaggag ccggatgttt gggacaggaa caacccaagg tttccgcgga accttccagg
     9661 gtcctcattg cctattattc atggggaggc aatacgaagt acgccgctgc gcaaattcag
     9721 agggcgacgg gaggaacgct gtttgaaatc aagccggtca agccttatcc ctccgaatac
     9781 cgggaatgca ccgtgcaggc caggaaggag attcaggaag gggttcggcc ggaactggcc
     9841 gccaaagtag aggatatggg caagtatgat gtgattttca tcggcagccc caactggtgg
     9901 agcaccattg cgcctccggt cgcgtctttc ctggcaagtt acgacctttc cggcaagacg
     9961 gtgattccat ttgtgacgca tgggggcggc ggcatggcgc gctgtgcgga cgaggttcgg
    10021 aagctgtgtc ccaagtccac cgttctcaag ggaggcgctt ttgccgggga aggaatccgg
    10081 acgacgaggg ccgcgcttgt caagtgggtc aatgagacga tttccatcaa taaataatga
    10141 ttttatgaaa cgaaccatcg cgggaatggc tttttccctt gcctgtctga tgaacctttc
    10201 agaagcccaa accataaatc acgataaagt ggaaatggag aatggcaaaa atacgggcat
    10261 gtgcgacaag ttaccgatga agcctgatgg tatcgtccgt ctctcaaaaa tagaagtgca
    10321 ttctgaatat ttggaggaat atgtgaaatg cgccatcgaa gtgggggaaa tctccctgcg
    10381 cacggaaccg ggcgtgttga ccatgtacgc tgttgctgaa aaggacaatg cttgcagaat
    10441 tacgattctt gaaacttacg ccagccaaaa ggcatataag tcgcacatcg catcggccca
    10501 ttttcagaaa tacaagcagg aaacgttaca catggttaag gcattggttt tatccgacca
    10561 gacgccgctg aatcctctaa acagtattac taatattatt aaataataca ataatgaata
    10621 agattatttt atttctttca attagttttt tcataacaaa tgctatggcg caggaaaaga
    10681 tagtacagac ggcgggacgc actcagttag gagaatttgc tcccaaattt gcggaactta
    10741 acgatgatgt ccttttcggt gaagtatgga gccggactga caagctcggt ctgcgtgatc
    10801 gcagtttggt aaccatcacc tccctcatta gtcagggcat taccgacaat tcgctcgttt
    10861 tccatcttca gtcggcaaag aagaatggag ttacccgtac cgagattgca gaaatcatca
    10921 cccacatcgg tttttatgcc ggttggccga aagcatgggc ggcatttaat ctggccaaag
    10981 acgtatgggc ggaagatgcg gctggcgaag acgccaaagc cgctttccaa cgcgagatga
    11041 ttttcccttt aggcgagccg aatacggcat atgccaagta tttcacaggc aacagctatc
    11101 ttgcacccgt ttcacgcgaa caagtgaata tctccaatgt aacttttgaa ccccgttgcc
    11161 gcaataactg gcatattcac aaagcgacaa aaggcggcgg gcagatgctt atcggtgtgg
    11221 ccggacgcgg ctggtatcag gaagaaggaa agcctgctgt ggaaattctt cctggcacag
    11281 ttatccatat tcccgccaac gtgaagcact ggcacggggc tgcctccgac agatggttct
    11341 cccacttggc tattgaggtt cccggtgaga atgcatccaa tgaatggctc gagccggtga
    11401 cggacgaaga gtatgccaag gccgtgaaa
//
LOCUS       DAWEMX010000082        11362 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2413_length_11362_cov_12.439197, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000082 DAWEMX010000000
VERSION     DAWEMX010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_11362_cov_12.439197"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    288..297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    737..746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8106..8115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2183 a         3015 c         3787 g         2347 t
ORIGIN      
        1 tcctctttga tgtgaacagg gggcgcaccg ccacggaggg tgccgttcag aagattccgg
       61 ggtatggaaa gcccgtggaa aacgtgacca aagatcagct ggttcaggga ttgaagactc
      121 cttatttcgc agaaccttat cctttgaatg acgagtgttt cctggccgtt tcctccccct
      181 ccggcaacca aggcgtgacc aatgtggtct ggtgcgacat atacgataac atcgttcctc
      241 tcacggcctc ctcctacttc gtctatgcag acccagcgcc cctggggnnn nnnnnnnccc
      301 gtgctgcatg accgggtgaa gacggaaaac aagacggcta cggtttatat ttccgacgta
      361 taccggggac gcgccatggc cggagtgccc agaggggaag ccaaggccct gcgcgtgttt
      421 atgtccgagt acagcccccg caatacggga agccactatg ccatgggcat ggaaagcaac
      481 tgggacttga aagtgctgta cggcaccgtg cccgtgaatc ctgacggttc cgccatcttt
      541 acggctcccg cctgccagcc tctgacgctt caggtgctgg acggggaggg gcgcgccctg
      601 gcgctgatgc ggagctggtt taccgccatg ccgggggaaa cgctcagctg catcggctgc
      661 catgaacggc agaactgcgc tccccccgcc agggccgtca tggcttcccg ccagaaaccc
      721 gctcctattg ttccgtnnnn nnnnnngtgg tacggtccgg cgcgcacttt ctccttcatg
      781 aatgaagtgc agcccgtgct ggaccgccat tgcatacgct gccacacagc ggaaagcaag
      841 gcccgtggag gagtgccgga tttcatgaca ttggaggccg tcaagggggc tcccgccccc
      901 ggttccgtct cttattggaa cctgcacccc tatgtgcgcc gcaacggccc ggaggggaac
      961 tatctggggc tggcccccac ggagtttttc gcggatacgt cggagcttta ccagctcctt
     1021 aaaaaaggcc accatggcgt ggaaatgccg caggaggact ggaaccgcct ggtcacgtgg
     1081 atggacatga acgcccccta tctgggcgaa tggccgggag agcgcaatga agggctgctg
     1141 aaacgccgtt atgacctgca cggcagattt tcaggagcgg aacgtaatta cgtgacgatg
     1201 aaggattccc tgttccggag aagcgccggc gtttccaaac ctgacgccgg gaagcccatt
     1261 gctcgcaccg gaggaaagag cgttcccgtt ccggaaccgc gtaatgaaac gctgactgtg
     1321 gatttgggcg acggcgtgaa catgacattc cgccgcattc ccgcagggaa gttcaggatg
     1381 gggaatgaac gggaaacacc ggcggaacgt cctgtttcca cggtggggat tgaaaggccg
     1441 ttctggatgg gagaggcgga agtgacactg gagcaatacc gccgcttcga cccggagtat
     1501 gctaacggat ggtatgacat gcattacaag gaccaggtga ggcccggcta cgacatggat
     1561 gccgatccgc gttttcccgt catccgggtg ccttgggcca gggccatgga attttgctgc
     1621 tggctttccg ggaaaaccgg gaaaaaagtg accttgccta cggaagcgca gtgggaatac
     1681 gcggccaggg ggggagctga tacggatttc tttttcgggg agcgggatgc ggatttttcc
     1741 gcgtatgcca acctggggga cgtgacgctg aaggaactgg ccgtttccgg cgtggatccc
     1801 aggccgatta aaaatcccaa ccgtttctgg gacttcgtgc cgagggatga gaaatacgat
     1861 gacgggaagc tccacctggc ccccgtcaag agttacacgc cgaatgccta cggattgtac
     1921 gacatgatag gcaatgccgc ggaatggacc cgttccgaat acaggcctta cccctggaag
     1981 gaaggggacg ggcgcaatga aggtctggac tcccgtgttc cccgtgcggt gcgcggcggt
     2041 tcctggtatg accgtcccag gagggccacg tcctcctggc gctggggata tcccggctgg
     2101 cgtccggtgt acaatgtggg gttccgggtg atcatagaag actgattgtt gaaattgcca
     2161 tgaaactgac aaaaatgttc cgcgcttttg cgactgccct ttgctgcctg ctggccctca
     2221 tttcatgcag gcagggaccg gatgctgctt ccggcccgct ggctccggtg gatgtgaagg
     2281 tcgtcgatca cctggttgcc gtgctggggc atgagagctg cgtgctggaa ctggtggatc
     2341 ctgcttccgg ggaaaaggtg aaaagcatcc ggctggccca gcctcccaac ggaatggccg
     2401 tggatggagc aacggcctat gtggcggaag gcggcccgcg cggagcagta gaagtagtgg
     2461 atctggaaag cggggcgttg aagcgttctt ttcctgccgg gcatacgccc atgtctcccg
     2521 tggtgcgggg ggaaaagctg tatgtggcct gccggtttga ttcccgcgtg ctggaaatgg
     2581 acgccgctac cggaaccgtg ctggattcct ggaatgtccc ccgtgaaccc gtagcgctgg
     2641 ccgtttctcc ggacggcagg aaaatctggg ctgccggcca tttgccggcc ggggctgcgg
     2701 acggggattt cacggctgcc gcgctgactc tggtggaaga aggaaaagct gtccatttcc
     2761 cgctttccaa cgggacgcag ggcgtgcgcg gcatggcgat aagcccggat ggccgctatc
     2821 tggccgtggc gcatgtattg agccggtacc aggtgcccac cacgcagctg gaccgcggct
     2881 ggatgaacac gaatgccgtg acggttatcg atacggacga gccggacaag ccccatcccg
     2941 ttctgctgga tgacccggat gcgggggcgg ccaacccgtg gggcgtttct ttttccggag
     3001 atggcggaaa actgtttgtg acgcatgcgg ggacgcatga actctccgta attgatttcc
     3061 cggccctgct ggagcgcatg aagcgggaag gccggggcaa tgagccggtt tccgaaaggc
     3121 ttggtttcct gcacggtctc cgcacccgga tcgcgctgcc cttgaacggc ccgcgctccg
     3181 tggcctccga tgggaaaaac gtttatgtag ccggctactt ttctgattct ctggcggaag
     3241 tctccctgaa ggatgcctgc aaatccaggg caattcccct gaacggccaa ttccggcctt
     3301 cccgggaaaa actgggggaa cggtatttta atgatgcctc ccattgcttc caggggtggc
     3361 agagctgcgc cacctgccac ccggacggcc gggtggacgg cctgaactgg gatctgctga
     3421 atgacggcat gggcaacccg aaaaatacgc gcaccatgtt cctttcccac cggacaagcc
     3481 cggtgatgac gctgggcgtc cgcgcctctg cggaagtggc cgtgacggca ggctttgtgc
     3541 acatccagtt tctggaacct tccggtgaat tggcggaatg cgtgaatgca tacctgaaaa
     3601 acatgaagga agttcccagc ccgttcctgg tggcgcatac cccttccaaa cagcagacag
     3661 ggggggaggg gtgcgcccag tgccatgctc ccggcgtgga aaggggttcc ttgtccgaat
     3721 ctgcccggag gggcagggaa gtgtttaaaa cagccgggtg cgtgcggtgc cacccccatc
     3781 cttacttcac taccaaggaa ctggtgtcca cggggacggc cacggggctg gatgaaggta
     3841 aaagcgttct ggtgccctcc ctggcggaag tgtggcggac tgccccttac ctgcacgacg
     3901 gccgtgcgaa gacgatccgg gaagccatca cgacatgcaa tccgggggat atccgaggga
     3961 agacgagttc cctgaataac cgtgaactgg aggatttgat caattacgtg cagtccctgt
     4021 aaaggggcgg gagggcatga tgacggcgaa tgcacctgaa caactggaat ggtccggaaa
     4081 aaacggggcg gaagaacgct tcttctgcat gacggcggag ccgggcgcaa gttttgagga
     4141 agaactgcat tccctgatga ccaggtaccg caatctgggg ggaggggagg aagatgaatt
     4201 cctgttgcgt ttccatgtca gcgacccggt gcgccagtcg gctgcgctgc ggcggatgat
     4261 aggggagagg aactcctatg tttccatggt tggccagccc cctgcgaacg gggcccgcgt
     4321 ggctctggaa gcctggcaca tcggcggaat gttgggaaaa aggcatatgg cggaacaggg
     4381 cggaacggtc gtggaagccc tccgcgctca ttaccggctg ttcctggcgg gcaagaggga
     4441 attctcctcc ccggacagct gcggccagat gcgggaggaa ttccgctggc tggaccggat
     4501 tgccgcctta caggggggaa ccgtgccgga tttgattcac cgcacctgga tttattgccg
     4561 ggacattgac aacaactacc ggggattggt ggaaggccgc aacggatgct ttgaccgtta
     4621 cgggctgact ccggagagcc atttcatcgc cagtaccggc attgaaggct gtacggagct
     4681 gccgggccgc ctgctgcata tggacagtct gggcatcgcg gggctggagc ccgggcaggt
     4741 ccggtacatg gaagcgccgg actacctgtc ccccacgcat gtgtaccggg tggcctttga
     4801 gcgcggcgtc cgtattgtgt acggggaccg ctcccattat ttcctttccg gcaccgccag
     4861 tattgacgcg gagggaaaca ttgtccatcc gggggacgtg gtgggccaga cggcccgcac
     4921 cctggaaaat atgagcgccc tgatggaaag gagcggggga agcctgtccg acctgaagca
     4981 ggccgtagtg tacctgcgcg actgggcgga ccgggaaacg gtccggaacc ggctgatgga
     5041 ctctccgctg gccgcagtcc cgcatgtgat gctgaaagcc cctgtctgcc gccccggctg
     5101 gctggtggaa atagacggaa tagccgttaa tggagccgga gagtccgcct ttgccccctt
     5161 gtaaatcctg ccgttcccgg aaccgcggcg cacgggcgat ttcccttgtt atcccgtgca
     5221 cggaaatggt atcatgaaag cgcatgactt atcgccttaa tacggaccgt ctggtatttt
     5281 tttccgtgct gttgtgggtg ctgttgccgc cgggtgtttt tgcctgggaa gggaatcctc
     5341 cgggtccgga taatgtgcgg tggctggagc agttctgttc cagctacatg cagtttctga
     5401 ccctgggcgg cgtgttgtgc gcggttattg gttttgtgtg cgccgccctg gtctggctgg
     5461 tgcatcgtct ggactgggcg cggagcttga aaatcagcgt gctgtttgtc atgtccatcc
     5521 tgtgcctggt tttgtatggc ggctcccttt cctttgccgt gttttatctt cccctggccc
     5581 tgattaaccc cgccgtgtat tgtttcagcg tcggccgggc ggaatgagcg tgcgggcagg
     5641 aatggtttta tctttctttt ccgtggcgga tatccgtggc ttccttcagg aagacgacgg
     5701 tggcggcgat ggttttggca atgtctccta tccgttccag agaacggata atgaagagaa
     5761 ggttgacgtt gagggaaggg gagtcctccc gttcggggga gaccagaagg gccagcgctt
     5821 tgcgatgggc tttgtccagt tttttgtcgc gcaggcgtat ttccctggcg gcgtccacat
     5881 cctgcgtttc cagacacctg gcggcgtctc tgaattcgga tacggccatt tccagcagag
     5941 ggagcacaat atccggggag agggattcct gatgcctgat ggaggccttg gcatgcttgg
     6001 cgatacccgt tacttcctcc atgcattcct ggagcttgtc cccgcatcgg gagaaggtga
     6061 tgacgagcct caggtcgcgt cccagcggat ggaactgggt gagtacggag gagcagaggg
     6121 agagaacctg ccgggtttct tcctgcagcg ggtcgctgtc cgcaatgatg ctgttggccc
     6181 cgtcgctgtc cgggccggaa atgacccgtt ccagaatgtc catgtatttc agcaggctgc
     6241 cgcagatggc attgacgcgg aggcggatag cgttcagggc cgcgtcgtag tgggggagga
     6301 tgtgattgcc gggggatgtc atgaggagaa tggtttggaa tgggctttta gctgaaacgg
     6361 cccgtaatgt aggcttccgt ttctttttcc gtaggattgg tgaagatttg ggacgtttcc
     6421 ccgtattcca ccaggcggcc catgtacatg aaggcggtcc ggtccgcgat gcgtgtggcc
     6481 tgctgcatgt tatgggtgac gatgacaatg gtgagttctt ttttcaggtc ttccatcagg
     6541 tcctctattt ttaaggtggc gatagggtcc agggcggcgc agggttcatc catcagcagg
     6601 atttgggggc ggttggcaat ggcgcgcgcg atgcacagcc gctgctgctg gccgccggaa
     6661 aggccgtagg cgctttcatg cagcttgtct ttcacttcat cccacagggc ggctcccttc
     6721 aggcttcgtt ccacggtttc gtccagttcc gcgcgcctgt tgcggccggc cacacggagg
     6781 ggaaagacga tgttttcgta aatgctttta gggaaagggt tgtatttctg gaagaccatg
     6841 cctacgcgct tgcgcaggga gatgacttcc acgcgcgggt catacaggct ggtgccgtca
     6901 atgcggatat ccccctggtg gcgcacgtcc gggaccaggt cgttcatgcg gttgatgttg
     6961 cggagcagcg tggattttcc gcagccggag ggcccgatga gggcggttac ccggttggtt
     7021 tcaaaggtca tgccgatgtt gaagagaacc tgtttgctcc cgtaaaagaa ggagaagtct
     7081 tccacttcaa tgatgggctc gtcgtctgcg gcggctggtt cagtcatggg atggtgaagg
     7141 agaatggggt tagaagctgg aggccgcgta ttttttgcgc aggcggttgc ggatgaggat
     7201 ggccgtgagg ttcatggcca ccaccagcag gatgaggagc agcgtggtgg cgaaaaccat
     7261 gggctgggcg gcgtccgagt ccggagactg gaagcccacg tcgtagatat gaaagcccag
     7321 atgcatgaat ttgcgttcca ggtggatgaa ggggccttcc ccgtcaatgg gcagggaggg
     7381 ggccagcttg accactcccg tgaccatgag tggggctacc tctccggcgc cacgggccat
     7441 ggccagaatg aggccggtca tgacgcccgg cagggcgctg gggaggatga tgcgtttaat
     7501 catctgccat ttggaggccc cgcaggccag ggccgcctcc cgcagcccgc gggggacggc
     7561 ggagagtgcc tcctccgtgg agacgatgac tacgggcagc gtcatcaggg ccagcgtgag
     7621 ggatgcccac aggatgccgg aggtcccgaa ggtgggggtg ttgtccacag ccagcttttt
     7681 ccagtagaaa agttcgtcga tgctccctcc cagatagtaa acgaagaagc cgaggccgaa
     7741 aacgccgaag acgatggaag gcacgcccgc caggttgttc acgcagatgc gcacggcctg
     7801 aaccagagtc ccctgtctgg cgtattccct caggtagatg gcgccgatga cccccacggg
     7861 cgtgaccagc acactcatga gcaaagtcat gatgaaggtg ccgaaaatgg cggggaagat
     7921 gccgccctcc gtgttggcct cccgcggatc atccgtgatg aaatggtaca ggttgtggag
     7981 gaagacgccg catttgccga agaatcccag gtcattcggc tggtagccgt aaacgatttt
     8041 atccatgcgg atttcctttc tttccccgtc tcccagggca tacacaagag agtctcggcc
     8101 ctgttnnnnn nnnnntgtac cgggcgtatt cggcattgag ctgttcctct ttggcgcgga
     8161 gccggtccag ttcaccggcc gggtcgtcca tatccgtgag aatggggttt ttcctgggga
     8221 cggccgtacg ctgtccgttt tcttcccgga tgtcgtagga acgttctatg gccttgatat
     8281 ccagtctgac gtccgccaga cgggtattga cggagccgat gtccttggtg tcaatggttt
     8341 tgaccctgtc cctcaggtct gtttcccggt ccagcacgcg gcggaaagcc tccatgaatt
     8401 ccggtgaggc ggcggggatg gtttctccgg aggccagctt gagttccaga ggtttgacca
     8461 gggtgttccc tccctccatg cgttccaggc acagaaggcc tttgggggtg gcggaggcag
     8521 tgacattgtg cgcgtccaca tagcggtagg attgtccata ggattccttg ctgccggtga
     8581 aaagctggta ttcccgcacg tctctggctg tggagccggg atgggcgggg tctgcgggga
     8641 cgtggcgcgt ctggtccttt gtgatgccgg cgtacaggac aagcggttgt tcttcttttg
     8701 aagaaggggc gagcgtcagt tcatggatag ttttgggcca gaaggcctcc agaccgttga
     8761 agacgatgat gcccagcagg ccgaaaatca tgatgaggcc cagggagagc ccgatggagg
     8821 cgagccagat attgacttcc cccaaggggc gtttgggggc gggcggaagg ctgaagtcct
     8881 gtttcatgga aggtggatgg atgggaggga aagggatgga tggttcacac gacgttgaag
     8941 cgtttgcgga gacgctggcg cactatttcc gccagagtgt tcagaatgaa tgtcatggag
     9001 aagaggataa ggccgcccag gaagaggacc cggtagtggg tggagtcctg cgccgcctcc
     9061 ggcagttccg tggcgatgtt ggcggagagg gtgcgcatgc cgttgaagat attccagtcc
     9121 atgatggggg tgtttcccgt ggccatcagt acgatcatgg tttcccccac ggctcggccc
     9181 agacctatca tcagggcgga gaaaatgcct gcggaagcca ccggaagaac aacggtgcgg
     9241 accatctgcc agcggctggc ccccagagcc tccgaggcgg cgatgaggga aggggggacg
     9301 ttgctcaggg cgtcctcgga aatggtgaag atgacaggga tgacggcaaa gcccatgatg
     9361 aatccaacca ctagggagtt gcgctgttca tagggcatgc cgaagccgtt gcgccacagg
     9421 tctgcgaaac tggcggcctg gaagtccccg tctccccaca ggcccatgac gctgcggcag
     9481 aggctgatga ggggctgttc cagccagtag cccaggtatt cccagcagaa ccaggcgcag
     9541 agcaggatga cgggggtcat gacgatgtat tccacaccgc ggctgaattt gttgcgccag
     9601 gctgcgggcc gggtggtcca gaaccatgcg atgagggccg ccaggccggg aatgaggacg
     9661 gccatgcaga gcagggcggg gactttgtct tccattctgg gggccaggta aagggcgccg
     9721 aagaagccca gcactacgga aggaagggag gccatgattt ccatgacggg ttttaccacc
     9781 cgtttgacgg caggagccat gaagtgggcc gtgtaaatgg cggctgtgac cgctaccggg
     9841 acggcgaaga caagggcgta cagggtgcct ttcagcgtgc cgaagaccag gggcatcagg
     9901 gacagcttgg attcataatc atccgtgccg cccacggatt gccagagcca tttgggagaa
     9961 tcgtacccct cataccatat tttcccgacc agggctttgg cgctggcttc cgggtgcttg
    10021 tcttccagag agaagaaatg gatgtttcct gatgggtcca cggcaatggc ggcgttgaat
    10081 tccgtattgg cggccagttg gaccggaacg aattccagag gtccgctgtt ccagcggatg
    10141 tcggccgtgg ttccgtagca gagcctgagt tcccgcggag tggagaccag gaaggaacgg
    10201 ttgatgccgg atgccgcgta atgctgcacc ggaccgtcca tgggagagaa ttgcctggtc
    10261 tggccgaaga ggcgcaggga agtgccgtcc gggcgggaat ggggataaag gctgaaaatc
    10321 ttaaggcttc ccctgtcgcc tcctatgacc agggacatgt ctccgaaaag ccagttgacg
    10381 gaagtcattt tttccccttt ccccagcggg gtcggaatca tctggcgtct gacccatgtt
    10441 tccccgtctt catcatagga gaagtaaaga agtttgtccg catccgtagc gatgacgagg
    10501 ctgtctccgg aggctccggg tagcatggcc gtggggcggc cttccagctg gtccgtcagg
    10561 tcatggaatc cggaaggcgc cagttctccc tcatgaagca gggagcggga ttcttccagc
    10621 gtcatcagca ggaggcgcgt gccctgttct gtctccacgg ttgcggtaaa tattttccgt
    10681 tctcccgcgt ccgcataggc tatgccggat attttgccgg ggacggagcc gctttccgtc
    10741 agggggtaga gagggccggc ctccgctccg gctttgtccg ggccgtgggc attcgtccgg
    10801 ccgtggacgt tgagtccgga atgaacatgg atgaggccca ccttgccgga ttccgtggcg
    10861 atggggtagg cggtgaggga tgcatcatag gaatgggcgc agacggtttc gccgtccggc
    10921 agggaaacgg gaacggagga caggttcccg gtagttttgt ccaggaagaa gatgtttttc
    10981 ccgttgccgt agacaaaggg aagcgtgccg gaagggtcca ggccccaggt tcccggaacc
    11041 ggagaggagg gcgaggggtg ctgccgttct tccacgtccg cgctctggaa gagaggaatc
    11101 gtaacggcca gcaggaagaa aagaatgccg aaaacggcta caatgacggt gaatcctccg
    11161 ccgatgatgg ttttggtcat gatgcggtca aaccacagcg tggtttttgc cacctgaaag
    11221 cgttccggaa cgggtttggg ctttctggaa ggtgcggggg agggagcatt ggaactcatg
    11281 acggcagaga ggagaagttg gagggcagga ctccggcggg cgggccggag tcctgccccg
    11341 gcatgtgttt acggcacggg aa
//
LOCUS       DAWEMX010000083        11362 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2414_length_11362_cov_11.750597, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000083 DAWEMX010000000
VERSION     DAWEMX010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_11362_cov_11.750597"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2114..2123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8913..8922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2797 a         3506 c         2859 g         2180 t
ORIGIN      
        1 gcacggtgga aggcagcatg cacacataca gcattcccat gcacaaatcc ggccccagca
       61 cactattcca caagggcatg ctgaacatac ccagcagggg gaaaataaca aaggtgctcg
      121 cagccaccag cccctgaagg cgccagtgca tcaacccttc ccatacggaa cggcgggaaa
      181 gcttggcccc gtacaggaaa aaaagcagaa cgatggctgc atcgctcagg cggctgaaca
      241 ctgtatccca tactccggaa cagggaacca gaattcccgc gcccacactg acgatgaggc
      301 cgatggtgaa gcgatccaat ttggcaagga atcccggcat ggagaaggaa aaaaggtaaa
      361 tgccgcctcc ggcgttctat ttgaaacgca cagcatgctt cttgctcagg cgcaggatgc
      421 tgcccggagc cagttccaaa acggcggagg ggtctgtcat tttcacatca ttcaactgga
      481 tggcgccggg cgtgaaatgc tccttgcgga gaacgccatt ggattttttc acctggaaaa
      541 cattctcaaa caggaaggaa accagggcca gggcattcat gccggcaggc agggaggcaa
      601 tctccacttc cggcaaatcc gcggcagcca aatccctctt ggaaaaacgg gtttcccagt
      661 cagaacgcgc ggcatccgca gcggcggaat catgatagcg tgccgtgatt ttccaggcca
      721 ggagcttttt ggcttccatc ggatgcaatc ccatgtcccg cttctcaccc agcagcacca
      781 ggtaatagcg gtccatcagt tcgtcgctgg cgctcatcat cttgccgaac atcatctccg
      841 gagcctcatc cacgcccacg taattcccgt aggacttgga catcttccgc acgccgtcca
      901 gaccttccag aagaggcatc gtcatggcga tctgcggcag catgccttcc tccttctgaa
      961 ggtcgcggcc caccaggatg ttgaaaagct ggtccgtccc gcctagttcc acgtccgcac
     1021 ggatttccac ggaatcccag ccctgcataa tcggatactg catctcatgc aggcgcacct
     1081 ccttaccgcc ctccacacgg gccttgaaat cctcccgggc cagcatctgc tgcatggtca
     1141 cgcgggaatt aagcttcagc acctcctcat acgtcatctt gcggaaccag tccccattat
     1201 acacgatctc cgtcttgtca cggtccagaa tcttgaacgc ctgcttggta tatgtctccg
     1261 cattctccag cacctgttca cgggaaaggg gggggcgggt cacggaacgg ccggaagggt
     1321 cgccgatcgt ggcggtgaaa tccccgatga gcaaaacagc ctggtggcca agttcctgga
     1381 actggcgcaa tttctcaata gccacggtat ggcccaggtg tatgtccggc gcagtagggt
     1441 ccacgcccag cttcacgcgc agggggcggc ccagcttgag gcgctccttc agctcttcgc
     1501 ggctgatcac gacggcggta ccgcccatca atatgtctaa ttgctcatct atagtcattg
     1561 atctggcgca tagagtgccg ggggcaccgc gccttttcaa gcctatatcc tgtgcgcggc
     1621 tcatcgaacg cagccaagag tcccccgcca ccggtcccga cgtgagggaa caggcacgaa
     1681 atccgtcccg aaaggcggaa taaacggcac gccggtgctg gcattttccc cgcatggccc
     1741 ttcctcccgt tccatgtcac ggaccagccc tgctcccttc cggatgcaga gaaacgtccc
     1801 cgcaaatcta cagaagctgg tacaggcggg gataggaagc atgattggcg gttatgtaat
     1861 gcgggcgcgg cttggggacc ggctttgtcc gcctttgatc ccagccgtca cggtcatgat
     1921 gataattatc atggtgcggc ggcccggaac ggtaataatc acgatcgtgg tggcgatgat
     1981 ggcagcctgc ggccaaaacg attgaggctg ctgcggcaaa aacaggaatt atgttcatct
     2041 tcatctttct tcagggtaag acagtccggc gggaggaatg cgaacggaaa aagtctgtca
     2101 cccgaacaaa cgcnnnnnnn nnnaccaact ccggcccccg accacacgct cccaggtgac
     2161 gaagaacacg gctcccaata cacataaaaa cgcccacagg taatcccact tccatttttc
     2221 tccgatgtag aacagggcga aaggaataaa cacgcacagg gtaatcacct cctgaataat
     2281 cttgagctgg gccacgttaa gcccgcccgc actgcccagg cggttggcgg gaatcatgaa
     2341 gcaatattca aaaaacgcca atccccagct catcaggata attaccagca ggggcttgcc
     2401 atccgccccg gaccccttct tcagatggcc gtaccacgcc cacgtcataa aaacgttgga
     2461 gaccaccagc atggaaatcg ttaacgcaat cttcatgatt tttatttaaa aacatcagtc
     2521 cttggaggac ttgggatcca gggcgtcccg caggccgtcc cccaggaaat tcagcgccag
     2581 cagggtagcc gaaaacagga gcgccgggaa caaaagcaat tccggactta cctccatgcg
     2641 gtccgcccct tccttgatca gcgttcccca tgaggaattg ggagccttca cgcccagccc
     2701 cagaaaggac aggacggctt ccagcagcat gatgccggga acggccagcg tcgtgtacac
     2761 gatgacggcc ccgaacaaat tcggagcaat atggcggaac agaatcttca tgtgaccgat
     2821 gcccagggaa cgggcagctt ccacaaactc ctgccctttc agctccatcg tctgcgtccg
     2881 gacaatacgc gccagcgtca gccagcccaa agcgccgatg gcgatgaaca ggggaatcag
     2941 cccggtcatg gggctcacca tttccacaga ccagcccgtc atgccggaaa cccactgcgt
     3001 cagcctccgg gccggctcct ccacggacaa ggaaaaaata atcaccagca caatgaacgg
     3061 cagggcaaaa agcacatcca ccaggcgcat catcagggca tccagcctgc ctccggcata
     3121 accggaaacc agtccgtacg ccacgccaat caccagggaa acgcccgtag cgaccactcc
     3181 caccagcagg gaaatgcggc cgccgtacag gacacgcgcc agcaggtccc tcccaagctg
     3241 gtccgtgccg aaccaatggt cccaggaagg gctttccgca atccgggaca aatcctgcac
     3301 attaggatga ggcagccacg gcagcagggg acccagaaaa caggccgccg caatcagcgc
     3361 caacgccagc agggaaaaca tggccgcccg gttgcgggaa agacgcagaa aagcgtcctt
     3421 ccacagggag gaaccctgtt cctccatcct gttccatcct ctttttctca tgattccttt
     3481 ctcaatctcg ggttgacggc catttgcacc agatccacac ccaggttggc cgcgacgatc
     3541 agaatgccgt aaaacaggac aagcccctgg atgaggaaat aatcccggtc cgtggttgcg
     3601 ttcacgaaat gctgccccat gccgggaacc tggaaacagg tctccaccac aaaagaaccg
     3661 gtaatcatgg cggcaaaagc aggccccaga taggaaaggg ccggaatcag gccgcctcgc
     3721 aaagcgtgct tccaaaccgt acggaaaggc cccgccccct tggctcgagc cgtccggatg
     3781 aaatcctgcc ccagcacttc aagcatgccg ccgcgcgtca ggcgcgccag atatgcggca
     3841 ttcaccagcc ccagcgtcag cgccggaagc accacacaac cgggagaatc ccatccggcc
     3901 acgctcaagc cgggcacatg catgcccagc gccacgccaa gcagcggagc cacgacgaaa
     3961 gcgggaatgc aaatgccggc catggaaacg agcatcaccc accagtccat ccaccggttc
     4021 ttgtacagtg ccgccacaat tccggcggga atccccgcca gaatggaaat agccatgcct
     4081 atgactccaa gctggaggga aacagggaat gactggccga tcatatccgc cacgctgacg
     4141 ccttccttca ccaaagacgg ccccaggtcg ccatgaacca gcatgttcca ccagtaacgc
     4201 ccgtactgga ccaggggagc ctgatccagc ccgtaactgg cctggagctg ctggaggaca
     4261 tgctccggaa gtttcttttc ccccatgaag gggtgccccg gcaacaggcg tatcaggaaa
     4321 aacgtcacag tttccagcac cagcaggacc agaagcccct gccccaggcg tttcagaatc
     4381 atcctgctca aggcgcggcc tccttcccat gttcccgggc gtagtccaga ctgatatcct
     4441 caaacaaatg attatccagc agcagggggt gccatccccg cacctccggc ctcttcaaat
     4501 aggaccgttt ggcccaatac aggggaatca ccggggattc ggaaagcatc agcgcttccg
     4561 cacgggccag cagggccatg cgcttctcct ggtctcccgt ggcgcggctc tccgccagca
     4621 ggcgttcata ctccggatga aaccagcccg tattattgtt cccggaggcg gaaccccaca
     4681 aatccagaaa actggaaggg tccagataat caccggacca tgaggaggaa ctaaaatcat
     4741 actgcatgga attctgcgcg aacttgtagg ccgtccactc acaggaacgg atatccacat
     4801 gaatccccag atgctttttc cacatgccct ggatggcttc cgccatcgtc ttctgaactt
     4861 cgcgggatgt agtcatcagc tccagccggg gaaaaccctt cccgccggga aaaccggcct
     4921 gggccagcag ggaacgcgct ttctccggat tgaactccac cccatgcggc gtcttgtatc
     4981 cggcgccgtc cggcgtaaag ccgaagcacg gctggccgcc gccgcacacc acgtcccgca
     5041 ccagagcctc ccggtctacc gccagggaaa gggcccgcct gacccgggcg tcattcagcg
     5101 gcgcacgcga cgtattcaga cggtaaaaaa tagtcacgta ataatcatcc tggcaaaaat
     5161 catcgccgcc gcgctcccgg gcgtaagccg tcatctccgg aggcacgttg ttggtgacat
     5221 gaagcttgcc gttgaaaaac atgcgggttt ccgtaaagcc gttcacggtg ggaagaaaac
     5281 ggactccatt cagccggacg gcggaagcgt tccggtaccg gggattgcgg cgcacctcca
     5341 cataatcatt gaaacggtgc tccgccataa gaaaaggtcc gttccctacg gccgctccgg
     5401 gccgcgtcca tttccccgtc ctgtccagca tgccgccgtg ggcttccacg gcatggcggg
     5461 gaaccggaaa ccaggtgcag tgaagcaaca gcaggggaag ctggggcatc ggagaacgca
     5521 gctccatctc cagggtataa tcatccgcgg cgcgcacgcc cacgcgggct tcctcccata
     5581 aatccggctt tccggcccgg acatcctcca acatccgggc cgcaatcttc cgcgctcctt
     5641 cctccggcat cccgtccggc cactccatca gggtaggatc atcccgcaga gcctccagct
     5701 cagcggctcc cataccatcc agcctctccc aatccacccg ggccagaacc ctctcttcca
     5761 gcccctcttc cccagccagc cgggaaccgg gaccgcacag cagcaggctg cgcctgttcc
     5821 ggttataggc ttccgcattc ttcagcggat aaagcatgtc cgcgtacctc cctccaaacc
     5881 ccggatgaag caggcggtga taggcatacg cgaaatcatg ggccgtcaca ggaacgccgt
     5941 cgctccacac ggcgtccttc cggagggaaa aacgccactt gtccgcctcc ggactatgcg
     6001 tccacgactc cgccacagcg ggatgaaaaa cggcgtcatc cctggaatcc ccgcgcagca
     6061 gcccctccat gacggcattg atcatcttga aatcggaaac ggaagtggcg atgtgggggt
     6121 caaagctctg gggctccgca ttattgccca cgatgagcac ccctgcctcc tgcgcccgtt
     6181 ccacgctcgt gcgcgtatcg catccggcga gcaaaagcat cacacagaac atcatgaaca
     6241 ggcgcatggc gggattctgt caggtttcct tcccgtacgc aaggcttcac gtcaagaaat
     6301 acggcggcag aaccacaacg acctgagcgc ccgcccagaa gcggaaggct cctcccagaa
     6361 cctttatccc acccggctcc cagttctgcg gcttaacaat atattttcct tcgtacgctc
     6421 atgatacata taaaatttca tagaaagaaa aacgcccctc ttccttactg gtcttttata
     6481 aaacggaggg ggtttatcct gttctcccgg ttattcctcc tctaaatttt tcccatgttt
     6541 aaattcaaac accggacatc ctgtatcttg accgtactcg cggggataat ctccattcag
     6601 gtagggaatg ccatcaccgt cccgtggctc agaccttata ttaacggtga agaagtggtc
     6661 agaactacgc tggaaagccg ttacagcaca cctgacggag aatccgccaa tcctgtgatc
     6721 cggtgggaaa aagcagatgt ccgaaacgga aaaaccgtag tggttcagca gagccgtgat
     6781 gcggaatcct acaccctgac ggacaaggat gcaggaaaat atttccggat tattgtaggc
     6841 tgcggaacca aagacgaagc tgccagccag tggatcggcc cggtcatcac ggaaaaacag
     6901 gcggaaagca tcaataaccg tttccatccc ggcaggagct accaggaaaa tgtgaacgaa
     6961 cttcaaaagg aactatctga aaaacttcga aacgccgtgt actttaccgt agatacggac
     7021 aagctgcatg gcgcaccctg cgcgatggcc agaaataaac gaatccctct gcccgaagat
     7081 atcagacctt tccgggaaaa cggaaaaatc tatatcagcc aaccctttgt acaggccatt
     7141 ttcaacaggg aactgcctga tgaaatcatg gaggaagcag ccggacagaa agcttatgaa
     7201 ataagcaatg tggccagggt tctgcaactc aacctctggc agggagacaa ggaaaaatct
     7261 cccgtaccga actttcgcat gcaacccatg gcggaaggac tggtgattct ctctcctgaa
     7321 aaagacatct tctctccagt agaagaccgt gacctgatta atgaagccgt caacgggctc
     7381 ttcgatttca aggccgataa taaacagtta aaatggtttc gggatgccaa attcggcttg
     7441 tttgttcact ggaacccttc ttccctgctg gaacgggaaa taagctggga acgcaatgcg
     7501 gcgcgtccca aagacagcgc gagcggtcat aagaataccg tagactttga gtacgactcc
     7561 gcccaccgcc gcttcaaccc caagcaatat gaccccagaa aatggatgtc cgtcgccaaa
     7621 aaaagcggta tgaaatacgc cgtgctgacc accaagcatc acgacggctt ctctaacttc
     7681 ccctccaact acgacacctt caccattgct gccaccccct acggcaaaga catcgtggaa
     7741 caattcgcgg cggcagttca tgcggccggg ctcagactgg gattttatta ttctggaaga
     7801 gactggtaca acccctacta cctgaccgac cagcactacc gttatctgga atactacttc
     7861 ggccaagtca gcgaactgct cacccgctac ggccaagtgg atgtgctctg gttcgacagc
     7921 ctgggcaata gctccctcaa ccagtgggat ccccgcacca tgatacgccg catcaagcag
     7981 taccagccgg acattctgat caacaaccgc atgaacagca cccgcggagg caataaggaa
     8041 ccgctgcccg atgatttgaa aggggatttc ctgacgccgg aatgcaagct aggccccttc
     8101 aacactcaaa cgccttggga aagctgcatg accgtggcgg atatccccgg cacccgctgg
     8161 acgggcaact ggtcctacac atccaacgca acaaccaaac ccgtcgaaac ctccatcaga
     8221 ttcctgatca ataacattgt caaggacggc aacctgcttt acaacatcgg tcccactcca
     8281 ctgggaacat ttgatccgaa acaggctgaa acttttctgt ccatgggaaa atggattgct
     8341 ttctacaagg aggccattta caacactcgc ggaggcccct acagggacca gccatggggc
     8401 ggcagttgct acagagcgga tcacaacggc aaaaaaaccg tttacctgca cgtcagcccg
     8461 ctgattgccc gacagggaaa agagctgaag ggaaataccc ccctgttcat caaagatatc
     8521 ggggaaaaat tcacgagagc gaccctcatt gtgaacggta ccggaaacgg aaaattcgtc
     8581 aggcttgaaa aggaaggaga ccagtacaaa atcactttgc ccgagggtgt cacctgggac
     8641 aggctggata ccgtcatcaa acttcaataa cccgctcttc caagaaaaca gcatgccttt
     8701 cccgcactct ctcctagacc ggatttttat taccggagaa tagtgttgcc tccacacggt
     8761 actcttgcct atcccatata cccggcctga cggaaccggc atcaaatcct gcctttcgcc
     8821 actcccgcag cagaagcgcc gaaaacacgc gccgcaggag aacagcgccc ctcataagcc
     8881 acagtgcctc cttttcatca tcccttttga gtnnnnnnnn nnaaacaggg ggaaagcaaa
     8941 accccgcaac ggcagaaaac cgttgcgggg ctgttttccc aaagcaaggg aaaagaaaag
     9001 cggctttaat tatttgccct gcgccttttt ggcagcgtca atctgggcct tgatcttatc
     9061 cttgttggca agaatgccgg ccttgatgcg gggggcctgc attccggctt ccgttcccgg
     9121 cttcaactcg gccaccttat ccagaatcag aacggcgccg tccacgtcac cggtggaatt
     9181 caggtaggtc ccgaactcca tcatcagcaa ttcctgctgt gtttccggca gaagcttgtc
     9241 gcggtcggga tatgccttaa ccacctgaag ggcttccgca ggcgtagtct tgtccgtcag
     9301 cttgccggca agataatttt gaacatcttt ccgttccttt ttaagctggt cggagagggc
     9361 gtctgcagct ttcaggccgg acttgtcatc cttgtccagc ttcttgattt ccgctttcac
     9421 gtcaccataa aaattgtcca catattcctg aggagccagc ttgagcactt ccatcagagc
     9481 ggcaactttc gcttcatccg tagcagcctt ggtaactttc gcttccgcgg cattaagagc
     9541 ttccttgttc ttcagggcat cctgcatggc tttcatgaca tcatccccgg aacgccctcc
     9601 aacaaatccc ccaaaaggct tgccggaagc gtccgcaaac acaacggtcg ggaatccccg
     9661 cacgccgtac tgctgggcaa gcttttcatt ggcggctttc atttccttgg actgttcctt
     9721 gccgcgcgga taatccagct ccaccagcac gaaatttttc tgcgcttcct tctggaagtc
     9781 aggcttgctg aatacgttgg cgcggagctg catgcaagga gggcaccagt cggaaccggt
     9841 gaactcaatc attaaatcct ttttctgttc ggcggcctgt tttttggcgg catccatatc
     9901 ggtaatccac ccttccgcgg caaagcaggg agcgacgcag gcggcaacaa caattggcag
     9961 aatttgttta atcatatcta caattcaata ttgtagaatc ggacagcccg cgtcaatccc
    10021 ctgttcaatc atttttagaa aatctccaaa agggtgcgtc ttccgcaggg aagtccgcat
    10081 acggccgatg ccggaaaaac tgatgcccca tgggaagacg gtgtcattat tgaagcaaat
    10141 cgggagttgc ctttcagcct gttttcactt atgtacagaa cctatcatga acacggtttt
    10201 aatcattgga gcaggtggcg ttgggcatgt ggtagccaac aaatgcgccc agctgccgga
    10261 tatttttcaa aacattcatc tggcctcccg cacgaagagc aaatgcgatg ccgttgcgga
    10321 ggacgtacgc tccagaacgg gcatcagcat cactacccat tccgtagatg cggacgacgt
    10381 gcccgccacc gtagccctca tccgggaaat caagccgcag ctgctgatca acgtagccct
    10441 gccctaccaa gatctcaccc tgatggaagc ctgcctggaa accggagtca actacctgga
    10501 taccgccaac tatgaacccc gtgacgtagc caaattcgaa tactcctggc aatgggcgta
    10561 tcaggagcaa ttccgcaaag ccgggctttt tgccctgctg ggttccggct ttgacccagg
    10621 cgtcaccaat gtattcacgg catgggctct caagcatcac tttgacgaaa tccatacgct
    10681 ggacatcatt gacgtcaatg gcggcaatca cggcaaggct ttcgccacca acttcaaccc
    10741 ggaaatcaac atccgggaag taacggcgcc ctgccgccac tgggaagacg gagccttccg
    10801 ggaaaccgct cccatgagca tgcaccaaag tttcacctgc ccgcaggaag tgggcaccta
    10861 tgaaatctac cgcatgtacc atgaagaaat ggaaagcctg gtcaagcaca tccccaccat
    10921 ccgccgggct caattctgga tgtccttctc accgaactac ctcaagcacc tggaagtgct
    10981 tcagaacgtg ggcatgaccc gcattgaccc ggtcatgtac aacggagttg aaatcatccc
    11041 gctgcaattc ctcaaggccg ttctgccgga ccccggggac ctgggcaaga cgaccagggg
    11101 aaaaacctgc atcggcaacg tcatcaccgg aatcaaggac ggccaattca aggctgtgta
    11161 catttacaac atttgcgacc atgaacaatg ctttgaagaa gtaggttccc aggccatttc
    11221 ctataccaca ggggttccgg ccatgatcgg cgcggaaatg atccttacgg gcaaatggac
    11281 tggcgcagga gtcttcaata tggaacaaaa tgatccggat cccttcatgg acgagctcaa
    11341 caggcgcggc ctaccctggc ac
//
LOCUS       DAWEMX010000084        11016 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2511_length_11016_cov_11.507709, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000084 DAWEMX010000000
VERSION     DAWEMX010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_11016_cov_11.507709"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2180 a         3046 c         3530 g         2260 t
ORIGIN      
        1 gcatggagtt gatgcgcagg catttcatca tgaaagtggg agcttttttc aaagcgtcgc
       61 cggaagaccg ggaagttctg ctttccggct ggaaaaagtg gctggagctg gtgggcgcgg
      121 agaacgtcct tccccgtctg gaccggtggg accgggcttt ttgccgcctt ctgctggatt
      181 gccccctgcg gagcaatttt ttctctgccc tgcattcccg cctgatgtcg tcccgcaagg
      241 ggcgccgggg ggcttactat accctgaaaa ggcacctgat gaagtgaaaa gggggagatt
      301 cccccctttc cctgttgttc cggcgccgca tggggttgcg gaaaggcgga atgcctgccg
      361 gcatcctaca ggcccgtgta gcacatctgc tctttgagct gggccacctg gaggtcttcc
      421 cccagatatt ccccgatgga gtagccgaag agcatggcgg cgccggggcc gttggcggtg
      481 agcatgttgc cgtccagcac cacattttca tctttcacaa tatgggcgcc ggcttcgttg
      541 agttcccggt aaacgtcctg ggccgggtag gccgtgacgt tcctgtccct gaccaggccc
      601 gctttggcca gcacgatggg ggcggcgcag atggcggcca ccagcttgcc cgcttcatgc
      661 atttttttga ccagatggat gacttccggc gtgtcgcgga gtacccagga gcccgcgccg
      721 ccgggcagga tcagggcgtc cagcttgtca gcatggagtt tatccagcat ggtgtccgtg
      781 ctgacggtta cttcatgggt gctgaccacc ttgtcggact ggacgccggc aagcactacg
      841 tccatgttca ggcgccgcag gatgtccagc ggagccatga gttcgatttc ttcaaatccg
      901 ggggcggcaa gtatggcgac ttttttcatg gttggtaaga cagggggagg aagaggaacg
      961 ttcaaggaaa agggcagcat gttccggtag cccctacagg aggagaagaa ccgggttttc
     1021 cagcagggtt ttgaattcgg cggcaatgtt ggaagccttg cattccggca gaatgccggc
     1081 gttcacgtac agggtgacgg gcaggatgag tttggacacg gggcgccccg cttcaatgcc
     1141 cgttttcggc gtggtgcctc ctatgctgat gatgctgtgg ggcatttccg gcagcacggc
     1201 gcgctgggct gccacgttca cgccgctgtc gcaaagaagg atgttgggcg cgacgctgcc
     1261 cgcggggaga gggcctgccg cgggggcggc caggcgttcc atggcgatgt tgtaaatcgt
     1321 cttgccggag gcgttttcaa tgaagacgta tttttccccc ttgtccagca ccatgagcag
     1381 ttccgcggcg gcgtctccgg ccagccattc cggctcgctg gcgcatgctt tcaccgccgc
     1441 gcgcaccacg tagtcaaaga ggctgtagcg gccgccgatg agtttttcgc actggacggc
     1501 gatgggcgtg ctgatggcgg ccaggtaagc catgttcgcc tccagggaat acaggtaaaa
     1561 gttgtctacg cgcgtggcgt ggcgcgtgtc ccggggcgcc agcggggtct gcacccggcg
     1621 ggcggggccg tccgcggggc gcagggcggc ccgtacatcc gcggccatga ttttgccccg
     1681 ggggccggtg ccgcgcagcg tggcggggtt caggccggct tccgccgcca gcctggcggc
     1741 cagcgggctg catgttgcgg acgggcgcgg ggggcagccg caggcggctg cgccgaattc
     1801 cggctgaagc agcgcaatgg gcgctcccgc cgcggcggaa cctccctccg gcaccaggat
     1861 ttccgtcagg gtgccgtcct cgcaggcctg gagctccaca tgggccttat ccgtctcaat
     1921 gtcggccaga tggtcgccga cttttacgaa gtcccctgtt tttttgagcc atctcaggac
     1981 ggtgccttcg tgcatggagt cggacaggcg gggcatttca atggtaatag ccatggaggt
     2041 gaatgttgaa atatggtaaa agggaggaag gcgcgccgga aggggcggga cgtctccttc
     2101 cggcgcgcgg aaacgcggtt acgcgctgta tttttccacg ccgtcgatga ataccttgca
     2161 ggtattgaag tcatcgtcca gcagaaccat gtccgccgtg aagccggggg cgattttgcc
     2221 caggccttcc aggcccaggg actgcgcctg gttccaggag gtggctttca ccaggtcgct
     2281 gaggggtttc ccggtcaggc ggtgcacgtt tttcaggccg tgggcgtatt tgagggtgct
     2341 gccggccagg gcgccggatt cctgcagacg ggccacgccg tccttcacga tgatgggcag
     2401 gccgcccagg tctccggggc cgtccggcat ccaggagcag gccagggagt ccgtaatcat
     2461 cagcatctgg tcagaatcct tgttcttgaa cacggttttg agcatgtccg cgcagaggtg
     2521 gatggtgtcg cagatgattt caatcttgat ttccctgtcc agcaggccgg accccaccag
     2581 gccgatttca cggtggtgca ggggggacat ctggttgcag tagtgggtca ggtgcgccag
     2641 ccccgcggac ttcgccctgt tgaagtcctc atgggtggcg gcgctgtgcc cggcggagca
     2701 gcggatgccg ttggccgtgg ctttctcaat gaattcaatg gctccgggca tttccggcgc
     2761 cagggagacc agcagcacct tggcgatgga gttcaggttg gctacttcat cgtaatccgg
     2821 ctggcggaca aaggcgggat tctgcgcgcc gcagcatttg gggttgatga acggcccttc
     2881 caggtggacg ccgggagtct tggcgaattc ctggttcttc atgtattccg ccacggaggc
     2941 gcatacgtct tccagcacct tgggggacag ggtgagggtg gtcggcagcc aggaagtgac
     3001 gccttccttc attttgcatt ccgcaatggt gcggaggctt tccagcttgg cgtcgcaggt
     3061 gtcgcagccg ccggcgccgt ggctgtggat gtcaatgaag ccgggaagga gcatgcggcc
     3121 ttccgcatcc acgaccttgt ctgcggcggg aagttcgctc ccggcgggat agacggcggt
     3181 aatggttttg ccttccattt ccacggcagc cccgggcata tcaatgccgg gggagatgat
     3241 gcgtgcgttt ttaatgagtg tcttcataaa aatgaatggt tgttggatgg atgtttatcg
     3301 tcgtttttct ccgttcgtcc gcgtctttcc gcctggcctc cgcttcctgc ggaaaaccgg
     3361 gaataggcgg cgcaccggag atcgttcatg ccgctggcag cttcctggtt atctggcggc
     3421 gtccagggcc ttgttccacc gggctacgcg ggcggcttcc gctttcagca cggcagccgc
     3481 ggcgttctta tccagaatgc ggatgtcctt gattttgtcc ccccggacaa tggagttgac
     3541 gacgtcctgc cctttcagca ccttcccgaa gaccgtatgg cggccgtcca gatggggcgt
     3601 ggccacatgg gtgatgaaaa attgggaacc gttggtgccg gggcccgcgt tcgccatgga
     3661 aagcacgccg gggccgtcat gcctgaggtc cggggaaaac tcgtcttcaa aactgtagcc
     3721 ggggcctccc atgcccgtgc cggtgggatc gccgccctgg atcatgaaat tcggaatcac
     3781 gcggtggaag gtgatgccct tgtagtaccc tctgctggcg aggttcagga agttggctac
     3841 ggtgacgggc gccttggagg ggtaaagggc cagttcaata tcccctttgg tagtggagat
     3901 gacgacattg acgtccttga ggccggctgt ggattccgcg gaggcgctga agcacgccaa
     3961 ggcgcacagg gaacacaaca gaagggtgag atattttttc atggcttttt gttttccccc
     4021 ctatgttgcc cgaataaaag cagggttgca agtaaatcgc gcgccctgac gggagaatgg
     4081 cgcggggcgc gccaatgcgg gaaggttttt ctctgaagag ggatttttaa ggatgggcgc
     4141 ccggttgccg gcccaggact accttgagcc ggaagccgcg cgggaaaaac ggaagaccgc
     4201 ccacgccatg aagaggctga ggattcccat gacgacgaat tgccaggagg gggggatgac
     4261 cagcatcatg gcgtattgga acccgacggc cagcaacccg agcgcgcagt ccaccaggtt
     4321 gccggcggca atcatgtccc cgcgtttgtc attgtccgcc cggtcctgga aaaaggcatt
     4381 cagaggcacc aggaacaggg cggcggaaat gcccgccgcc atcagcaggg cattaaagac
     4441 cggggaagcc atggagactg cggccagggc cgcgcagcta gccgccatca gcacgcctcc
     4501 cgccacggaa acattcatcc ggatgtggcg gcggcaaatc acggaggcga gaatgccccc
     4561 cagcaccgtt cccccgctca tccaggccat cagcacggct cccacggagc tgaagtcatc
     4621 cccgccaccg gagatttcct tggccatctg gatggtcatc agcatgaccg tgccgccgaa
     4681 gaaccagaaa tagccgatgc ccatctcgct gacgcgcaga ttgcggtttt tccacagctt
     4741 gcccagctgg tggaagtgct cgtaaaacag ggaccaggag aaggggcggc cccccccggg
     4801 agcgtaccgg ggcaggcgga agctggaggc cgccaccgga acggccagga gcagaaacac
     4861 caggcatggg aaagaggcgg cataccatcc gtcattgatg ccggagggtt tcagccatgc
     4921 gtcaaaccac tggaagaagc cctcccccag ccagcggtgc cagatggtat ccgtggaagg
     4981 ctccagcagg tagcggaacc agatgaagac gccgatctgg ccgatgagca ggctgaggat
     5041 ggaagccatt tccacgatgc cgctggcgaa ccccatgcgg gaagttccca cgatgtcctt
     5101 gacgattccc tttttggccg ggctgaagac ggtggcctgc acggcaaaga cgcagaacca
     5161 caggatggcc ccgttcagat tgtggacgcg caggctccag tacatgcccg ccagcacgca
     5221 tatctggagc agggccatga attggatgat gcgcgccttg cagaaacggt ccgaaagcca
     5281 gccgacgaat ggggagaaca ggatgaaggg cagcacgatg atggccccca gcggatattc
     5341 caggtccccc tctgcgccgt acagccagac gcccagggga atcagcagga attgagcccc
     5401 tttttcatta aaggagttct gcgcctgaat cagcacgagc cgccagaaag ctccccatgg
     5461 aactttgcgg gcgtgtcctg gcgtgtggac gctggaattc atgaagttac aggctgggtt
     5521 cctccgcttt ttccaggccg gagccgcgga acaggcacag cacgccgatg acggcggtca
     5581 gcagggtgct ggcaatcagg gaggcttctt cactgaaacc gcacatttcc gggctggaga
     5641 acagatattg gaatactacc aggctcagga aggcggcggg ggccgccgcc ggccagtagc
     5701 agcgtttttt gctgcgcagg taaatggaaa cggcccagag cgtggcggtg gccagcagct
     5761 ggttggccca gccgaagtac tgccagatga cggcgtagtc catgctgctg acggcaatgg
     5821 ccgcggcgaa gagagggatg gcgatcatca ggcggcggct cagctgttcc tggctcagct
     5881 tgaaactgtc cgcaatcatc aggcgcgtga cccgcagggc gccgtccccc gtgctgatgg
     5941 ggaggatgac cacgccgatc atgaccagga tggaaccgac gctccccatc caggattcgg
     6001 aaatcgtctg aatggccagc gccggagcct ttccgttggc ggtagctcct cccagggctt
     6061 ccggagagcc gtagaaggtc agcgcggcgg cggcccatat gaaagcgatg atgccttccg
     6121 taatcatcgc ccctccaaag acgggcagcc cttcccgctc cgttttcaga caccgcgcca
     6181 tcagcgggga ctgtgtggca tggaagccgc tcacggctcc gcaggcgatg gtgatgaaca
     6241 tcacggggaa aagcgggaaa tccgccggat ggcggttatg gaaaaagtcc agatccggaa
     6301 gcacttccat gcggggcagg tgcatccagg ccggcatgga atccgcaaag ggagccagga
     6361 gcatcacgcc cagaatgccc atgaccatga tgatcagggc gacggaaaaa agaggataga
     6421 cctttcccat gatagcctgg atgggaagaa tggtcgccag gaagtagtag ccgaagataa
     6481 tccaaatcca tgccgaaacg ctccagcccg tcatggggga caggatggcg gcgggcccca
     6541 cggcgaagac gacgcccacc agcacgctga atacgatgca gacggcgcgg ctgacccact
     6601 gggcctggct gcccaggtag cgccccagga tttccggcag gttttccccc ttgtgccgca
     6661 gggaaatcat gccggagaag tagtcgtgcg ccattcctcc cagaatgcag ccgaaaacga
     6721 tccacagaag cgcggccggc ccgtacagca cgcccatgac ggcgccgaag atggggccca
     6781 gccctgcaat gttcagcaac tggatgagaa aaacgcgcca gcgcggcatg accacataat
     6841 ccacgccgtc cgtacaggcc acggcgggag tttgccggga ggcgtccgga cggaaaatcc
     6901 tttccagcac ccgcccgtaa gtgaaataac cggccgccag gaccaatatc cccagaatga
     6961 aatagcagta accgttcatg ttggaaaaag ggccagccat ctcatggaca ggctgtccgt
     7021 ccacggaacc taacttttct cccgccttct ggcaagccgt tagtgaaggg agggatatgg
     7081 aacggattcc taatccgaaa aataaatcca cgtctcttta ttatgcataa ataattggat
     7141 ttgtgaattt tatgatagtt tcaaatctcc cgcaggaaaa ttcctggtta taagactcct
     7201 ctgttcatgt atttgtataa acatctcgtt tttatcgttt agtgttgact tcggattagg
     7261 aatccgctaa acggaaaagg ttgacaccat tcaacctttc tcagttatga agaaaacatt
     7321 gataatcatt gcgtccctgg ctcttggcgc ggcggcttcc caggccgcgg tgctgtgtac
     7381 cactacgttt aaccgaaccg ggacttccct ggataccgtc actgtgagta cggtctccga
     7441 tgcgggactc tccacttcca cctcgattgc ttccgagaat ttcagcaaga gctctgcggg
     7501 cagtgacctg ctggcttcag gttctattcc ggcctccgta ttctccccca attccaacgt
     7561 gggtaataat cagaataata cgtggagcgt ttctttcacg tttaccaacg acggctccca
     7621 ggatatgctg atttcttcca ttgatctttc catgataggg ttcaatgggt cgggcgccgc
     7681 gcagaatgcc gggggcggtg tggccaataa caattatgtt gggggagccg acggcaattt
     7741 gaacaaacct gtcaatatca cccttggcat gagcgggcag gaaaatcaga cgctggccta
     7801 taatggcgcc accagtacaa atacatccac gggatcctgg gatggcgttc atacgggaag
     7861 ctatgaatat ggcgatgtgt tgttgaaagc cggggaatcc atgacgttaa ctgttacggc
     7921 ttccaaaaac gaggcctacg gcagcggctg ttttatcggt cttactggaa ttcagattaa
     7981 tggggaagtg gttcccgaac ccgccacggc ctccctgggg cttttggggc tggccgcatt
     8041 gatgatgcgc cgccgcagac tctgagtttc gtgtccctgt atcatttgtt caagccgtcc
     8101 gtttttcttt aaaactggcg gcttgttcat tttctgccgg caaaattcgg catttttaac
     8161 atttaacatc aataaccggt atgattcccc aggcagtccg ttcttcggcg tgatgtcctc
     8221 cgggtcatcg tttcgccagc cagccatgtt cccgcaccgt tttttttccg ttctcctctt
     8281 ttttcttctg ttccttgctc cagccaggtc tgctgatgtt gaatatgtct ggaggggtgc
     8341 cgattaccag tggggttccc tgtccaactg gagtgtcgga ggcattgccg cctcttccgc
     8401 gccaggggcc gccgcttccg cctatgaaca ttggatggta accaatggaa cggattccgc
     8461 gggaaggacg gatgtcggcg gattgggcgc ggggggccgc tatttgaagg gcgtcaggat
     8521 tgaggggctt aacagccagc cggaaggcaa aatcccattg ttcattaaaa acacgaacaa
     8581 ggatgtatat ttgcgcgtgg aggagggcgg cattaccgtg gaaaatgccg gagaaggcgg
     8641 ttattctgcg gatttcgggg tcgcccagct gcgcgtagcc gcggaccagg agtggcatgt
     8701 ggctgaaggg cgcagcctgt atgtggggca tgacgatgac gctccgtccg ggggattgta
     8761 ttccctgact tcggaaggcg atgttccgcg gcgcgtgaca gtgacgggcg gaggcgccgt
     8821 gcgcatcggg gaagggatgc tgctgaacaa tatttccggc ctcatcggtt ttgtactgaa
     8881 cgccggaaag ggaattccga cgctggatct ggcggaccgg ggcatgggca atacggtgac
     8941 ggtggaagac gctgcccgcc tggaaggcat gtctttgtac cagggggccc tggtgacgcg
     9001 ggaaaacgcg tccgtgactt tttccggaac ggaggccaag gcgtccggac aatggaacat
     9061 tggcgcggac acggagttgg cgctggaaaa ttccacgctg gacctgacgg aggcgggtgt
     9121 ggacggcaac gtgattcttt ccggctcttc cggcattacc ggggataagg gaacgctgcg
     9181 gcagacgctt ctggatgatg cccgggtcac ttatacggac aggaatgtca aggcggggga
     9241 aatccggagc gtaggcagca atgtgacgat aacgctgaat aattcctcca tgcattttga
     9301 cggagaaatt ccggaggtgg acctggtggt gcagggcagc tgcgcactgg gcggcagcgg
     9361 cgtcttttca ggaacgatta cttatgcgcc gggaggcacc ctgactgtgg atggggatat
     9421 ttccctgccc agttcatccc tgacgctggg gcgtctccat gtggctacgc tggacggcgt
     9481 gccgcaaaac tccgccgtcc ggccggaaac ccctgcccag caggcaaggg aatatgtggt
     9541 tttgtttgat tcctcctttg aggatttgat ttccgaatgg ccgggcaggc atacgctggg
     9601 cgtgcagttc cggccggata tgggcgcccc cgtcaccgta aaggaactgc ctgaaggggc
     9661 ggtttcatgg aattatgatg ccgcatccgg ctggctgacg ctgcagacgg atgtagcgga
     9721 aaagccggac atgccggcgc atgtttccgg agccaggccc aacattattt ttgtcctggt
     9781 ggatgacatg ggctgggggg atatgagcgt caactggacg cagcaggata aaaacggcag
     9841 gcaggtgacc cgcaggaatg aattcaagac gcctaccctg gacacgatgg cggcggaggg
     9901 catgcagctg agacgccatt acagtgcggc tccggtatgc gctccggccc gcgcttccct
     9961 gctgctgggc gttcaccagg ggcattcccg ggtggtgcgc aacaatacgt ttgattaccc
    10021 tatcgagaat tcccatacgc tgggaacgct gctgaaaagc gcgggttacc atacggcggc
    10081 catcgggaaa tggggcgtgg gcggaggcgg acagagcggc aaggctctga ctgcggctcc
    10141 ccacatgcgc gggtttgatt atttttacgg aatcatcaag catttggcgg ggcattacca
    10201 ttatttgacg gcgggttccc aggatattta cgaatataat gaccaggccg cggctccggc
    10261 gtgggagagc gtcagcgcga aagttccggg taccgcctat gatacggatt tgttcggggc
    10321 ccgcgccaaa cagtggattg tggaccacca tgagaaaact ccttcccggc ctttcttcct
    10381 gtatctggct ttcccggctc cccacggcag tctgtccgct cccgcctgtg cctatccggc
    10441 ggggctggga agggacggcg gcctccagtg ggagaaaaaa gacggctatg aagcctgcaa
    10501 taccgctgcg gacggatggt cgggggtgga gggggatttt tctccggata cctatatcta
    10561 cccggaacat gaggtttttg gaagaacgga atcccgccac gccacgatga tccgccgggt
    10621 ggatgacgtc ctgaaggaca tgatccagct gcttaaggat ttaggcattg atgacaatac
    10681 gatgattgtc ttcacgtccg acaacggccc ccacaatgaa cccggttccg gcaattacgg
    10741 caacggcgcg caggatcccg cgtatttcat gagctacggg atgatggacg gcattaagcg
    10801 cgattgctgg gaaggcggca tgcgtgttcc ggccgtggta cgctggcccg gtgtggttcc
    10861 cagcggaatc agtttgaatg ccagccagtt ccaggactgg atggctactt ttgcggacgt
    10921 ggccggagtg cccgtgccgt cccgttgtga cggcgtttcc ctgctgccta cgctggcagg
    10981 ggtgccggaa cgccagaaaa cgggcgttat ttatgc
//
LOCUS       DAWEMX010000085        11009 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2514_length_11009_cov_11.026292, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000085 DAWEMX010000000
VERSION     DAWEMX010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_11009_cov_11.026292"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4918..4927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5338..5347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2492 a         2818 c         3235 g         2444 t
ORIGIN      
        1 aagctgtgtt gatgcggcag atgaaaataa tgacgggcag aatgaaagga aaatcatgga
       61 aatgcgtatc cttcaacaaa atagaccgct tcatggttaa gtgcaggtgt tgggaaaacg
      121 gcggtttttt caggctgccc ggtttctggc ggcggttttt tctcaaagaa cgacatgaat
      181 ggttttattt tgtggatgct ttgctgcgct tcttggagcc tgggagcgga tatatttgtt
      241 tctcctgacg gagatgacgc tcatgcgggg acggaagaat ttccgatgcg ttccgtggag
      301 gccgctgtgc ggaaggccgg gcaagggcat tacggaaaag gctgccagat tatcctggcc
      361 gacggtattt atcacctggc ggaaccgctg ctcctgcgtc cggttcattc cggtctgact
      421 attcgtgcca agaatccgca taaggcggta ttgagcggtg ctcagccctt tgttgcggac
      481 tggaagccct tccgtgatgg gatcatgaga acttccgtgc cctccggact ggaaatggac
      541 gtattattgg tcaacggcaa acccatgcac atggccaggt atcccaacgc aactcccgaa
      601 gaacgcattt tccagggaac ggccccggac gcggacgctc cggaacgggt gtagcccgct
      661 ggaaaaatcc ggcgggtgcg tatctgcatg cccttcatca tcgcatgtag ggagacatgc
      721 acttccgcat tacggggaaa aatggaaata cactggtcaa gcaggggggc tggcagaata
      781 accgtccctc tccgccgcac gaaaaatata aatttgtaga gaatgttttt gaggaactgg
      841 atgctcccgg agaatggtat ctggacagaa aggaacatat cctttacctg tatccggaac
      901 agggaacgga cctggttgca gccagactgg aagccggcgg cctggaagaa ctggtcaaaa
      961 tccaggggga aggctcatcc cccgtcagga acgtgacgct ccgggatctg gtgctgactg
     1021 gaacgcggcg aaccttcatg gaaaaccggg aacccctgct gagaagtgac tggaccattt
     1081 tcagaagcgg agccgtactc ttgcggggaa cggaggggtg ccgcattctc tcctgcgaat
     1141 ttgccaggtt gggtggaaca gccgtttttg tggacgggaa taatgaaaat cttctggtgc
     1201 ggtcctgtta tatccatgac ataggttcca acggcgtagc gtttgtgggg gaccgttcct
     1261 gctaccgggg accgaagaat tatcgggaag ccaaaggaat gtccttggag agaatcgaca
     1321 gggttccggg acctcgaaac aatgaatttc ccaggaactg catggtggat gattgcctga
     1381 ttacccggac ggggctggtg gaaaagcaga ctgccggagt gcagatatcc ctggcccggg
     1441 aaattaccgt cagaaattgt tccatctacg aacttccccg ggcgggtatc aacataggag
     1501 agggggcttt cggcggccat gtgattgaat acaatgacgt ttttgatacc gtcagggaaa
     1561 ccagcgacca tggttcgttc aactcctggg gacgggacag attctgggtc cgggaccaga
     1621 tggcgctgag ccaggacaag gacgtcgtcc tgctggatat ccggcaacct aatatcatca
     1681 ggaacaaccg ctggcgctgt gatcacggat gggatgtgga cctggacgac ggttcttcca
     1741 actatattat ttacaacaac ctcatgcttt cctccggcct gaagctccgg gagggggggt
     1801 accgcaaggt ttacaacaat attatggtta acaaaaccct gtacccgcat gtatggttca
     1861 ggaacagcgg ggacgaattt tacaataaca ttatttttga agaccgttac cgtcccgccg
     1921 gcaacatgga tttttccccg tggggaaaac tgatggacag gaactttgtg catgtgaagg
     1981 gaatgaaagg ggtggaaccc gcctccgaac tggccaggca atccggcaat gacaggcact
     2041 ccctgaaggg ggatgccctg ttctctgctc ccggtctggg ggatttttcc gtccgggctt
     2101 cttcccccgc ccttaaactg ggattccgca attttcccat ggaccgtttc ggcgtgcgtt
     2161 cccggcatct gaaagccctg gcgcgaactc cggatattcc tgaagttgcc gggaacagga
     2221 tggagaaacg ggaaacggtg ctggtgaaga aactgggggc ggaagtgagg atagcggagg
     2281 gcgaagggga tttgtctgtc tttgggctga tgccggagga tctgggccgg gcgctggtta
     2341 ttgtcaaggt acagaaggac ggcccttgtt cctctgccgg cattctgccc ggcgacgttc
     2401 ttctgatggc gggcgggaat aaggtggatg gagtggaaaa gctcgaacgc ctgctgccgt
     2461 cttccggtaa attaaccgta accgtcagga gaaaccagga aaatagaaag gcggatttgc
     2521 ggttttgagg atgcttctgg cctccggaaa aagaggaagc ggaaattaca gaattccggt
     2581 caacttccgg taagtctcca gggcaaaaga atccgtcatt ccggaaatat aatcaagcat
     2641 gtgctccagg cgtgcagcct tcccgccgtt gttccggggt acgccggtgc cctgaagcat
     2701 gatggcgatt ttttgggatt ggccggagga tggatgattc acccattcca taaagaagtc
     2761 gatcaattct cccagtacct tataacccat ggcttccact tcaataacct ccctggcctg
     2821 gtacacattc ttgatggcgt actggtacaa gccgttcagc ggggcggcca gttccgagct
     2881 gtctatcagg gattgttcca accttccttg cagcaggtct tcctcgtggt tcacgaaact
     2941 gtccacggcg gactgaatgc ttttgccgat ggcgagcgcg cgcatataat ggatgcagga
     3001 atctttttcc tcttttgctt ccagttcccg gacataacag agctgggatt ccgtcaggaa
     3061 agggctgaac aaatccctgg tggaagcaaa atccagaagt ccggaaaaat acccgtcctc
     3121 aatatccgca atgcggtaac agatatcatc cgcggcttcc atcaaatacg ccaggggatg
     3181 gcggctccag caaagatgtg ctgcatcagg tgtggaacgc ggaatcaacc ccagatgtcc
     3241 agccatgaaa gaagcagctt ccctttcctg ttccccgatg ccgaatttct tgcattcaag
     3301 cttgttggcc gcaactatgc tgcctttcct gacggcggag gaataagatt gcgtacaggg
     3361 atatttcata aaggcgccca aaacagccgc cgtcagtttc aggccgttgc cctccatcgg
     3421 atctcctgta cgtgtcagga tgcggaatcc ctgggcattc ccttcaaaag gcagctccag
     3481 gccgtgccgt ttcagggcgg tttcaatggc gtcttcaccg gaatggccga agggcggatt
     3541 gccgatgtca tgggccaggc aggcggtgga aacaatggta gcgacatcgg aaggcatgat
     3601 aggagcgggc agggaccctt tcttcgccag ccactcccct gccagcatgc cgagggaaga
     3661 accgatgtgg gctacctcca ggctatgcgt caggcgtgtg cgcacgtaat cgttgcggcc
     3721 caggggaaaa acctgtgttt tgtcctgaag gcggcggaaa gcactggaaa acagaatgcg
     3781 gccgtaatcc cggtcataat tgctgcggtt tttctgtgga gatgccgtac tccgatgcct
     3841 ggatccccag cgcgcgtccg acaaaagctg ctgccaattc atcatccctg acatggtaag
     3901 gaaatgctac aggggaatgc ccggatgtaa caggagaaaa atagagaggg agttcaatag
     3961 gtcagcatca gccccacgcc ccagtgcagg ttcttgtcgt aattggtgga aacgctgagg
     4021 tgtgaggtca ggatgtagtg caggcccacg gaataatccc ggtccgtatt cagggaaaag
     4081 gaaaggcgca ggcgggaggt gagcgggata tcgtcccgct ccaattgcag gcgcaccttt
     4141 ccgtccgtgt caatgcgcgc atccgcaatc aggagcaggg gcaacatgta ccgggctccc
     4201 agggtgccat ccatttcccg gtcctttttg gtggtctgtc ggaacatgtt gcgttctccg
     4261 gcctccccct tgcggcaagt ccagtccgcc ccgatgtagg ggtaaagcca ttgcttctcc
     4321 ccgatatacc tgcccagcct cccttccgcc tcataacccc gcgtatcctt gtagcctatt
     4381 ttccactctc cctgcatgct ccaccgggtg cctccgaatt ccaggttccc tatgttcccg
     4441 ttggtggcga aatcattgtt cacgctcagg taccatttcc ggtccatgtc gtaaacatgc
     4501 tgaagtgcct gcatggggtg gggaagctga ggattggggg gagaatcctc atacgtaaaa
     4561 atccggccca tgccgctcat catgtggtac aggatatggc agtggaaaaa ccagttgccg
     4621 cgcgttttct ctgcagcgtt aaactcaatc acctgggtgt ccatgggctg gatatccgct
     4681 gtaaacttga gcggagaaaa atccccatgc ctgttcacca gccgaaaaaa atgcccgtgc
     4741 agatgcatgg gatgcctcat catggtattg ttggtcagga ttatgcgcac attctgtcct
     4801 tccctgatca tgatccggtc cgtctcggaa agagttttcc cgttaatgct ccatacgtaa
     4861 cggttcatat taccggtcag catgaaatgc aattccttaa cgggcacgcc ggaaggannn
     4921 nnnnnnnact tcagcatgtc atacgtcagg gtggtttctt ctctgtcctc ctgcatgttg
     4981 ccgtgtcctc tcccatggga ggaaccggac ttcatgtcca ttatctccat atttccggaa
     5041 tgggcggtcg gcatgccgtg atgatgcatc ttcatatttc ctcccatggt catcatccca
     5101 ttcatttgct tcatcttttt gaagtaattc agcttgggaa aggggcgcaa aggctgcctt
     5161 tccccatggc ccagccacag ggaggaagac ccggaagtat cctctgccgt cgcctggaac
     5221 tcaaaggcct tgcctgattc cggcacttcc aagatgacgt cgtaagtttc ggaaactgcg
     5281 attatcatgc ggtccacgtc aacgggaaca acgtctttcc cgtcgctggc caccacgnnn
     5341 nnnnnnntca gccagaaata ggtggaggat gctccgttga cgatgcgcag cctgatgcgg
     5401 tcgccgcccc tcagccgggg cagggaagcc cgcgggatgc cgttgagcag aaaacattca
     5461 taatacacgt cactcacatc catggcgttc atgcgcttcc attcgcttaa cagcttggtt
     5521 gccatgcagc cggagcgcag ggcctcccaa tagctctgta cactcccctt gcggatggaa
     5581 aaccagtcgg agcccgtgtg aagcattctg ttcacctcct ggggattctc gttcgtccag
     5641 tcgctcagta ccagcgtata ttccggcagg gcgtcctccg tcctgcgtgc cgggtcatcg
     5701 ggccgtttgc ggacgatgaa cgctccgtac aggccgctct gctcctgaaa accgctgtgg
     5761 gaatggtacc agtacgtgcc gttctggatg atgggaaaag tataggtatg cgtagtgtgg
     5821 ggcttgacag gagcggaagt caggtacgga actccatcct ggtcattggg caccagcaag
     5881 ccgtgccaat ggaaggaagt ttccgtgtcc atcaggttat gcacccgtat taccgcagta
     5941 tctccctctg taaaatgcaa tgtggggccg ggaatgttcc cattgatggt catggcttgg
     6001 cgtgtgactc cggtaaaatt gacgggagcg ttccggacat ataaatcgta ttctaccgtt
     6061 tttgccagag aaggggagaa gagtatcggc aagcagcaca ggaatggaag aaaatggaag
     6121 gttcgtgtga acatgaatgg gaaaaagcac tgccaggaaa gacctgcctc ttgaatttcc
     6181 agttcaaaat gaaacggatg tgatgtgtca tgcccaccct tggccaaatc tgctaaatgg
     6241 cggggcctcc aattcggacg gaatagggcg tctggaattt attttatgcg tcgtttttta
     6301 aggaaatagg gacgatcagg aaagaggcca ggataagcag gcagccgatg gacagaagga
     6361 acatgttgga atgctccctg aacaggagca gggaagtcat ggccgccagg ggaatcttga
     6421 gattgttcat aatcgccagc tttacgggag ttacccggcg cgcgccgtaa ttccacagga
     6481 aaaaacagac tcctgaagcg atgacgccca aataggtcag cagccaccat tgcacggttg
     6541 aaatcccctg ccagcaggcc agtcctgacg gcaggccgga aggcagagta accagcaggg
     6601 cgcctgcata ggcataggcg aagcaggatg cttcgcttct cccgaaccag gaatttttca
     6661 gggaaatgta ggctatctga cctgccgcga agcagaggtt ggaaagctgg accagaacga
     6721 ttcctgtcaa ctccccgtta ccggaggaat atcctttcca aaggattccc gcccctccca
     6781 tggctgcaag aatagccgcc gccagatgga gcggcggaag attctttttc atcaggccgt
     6841 ctattagaac cacataaatc ggcgtggtgg ctgtaaaaag ggcaacctgg tgggaaggca
     6901 gatagtggaa ggcagcgata tagaaaagat acatccctcc gaactggacg gcgcctatcc
     6961 ccatcatccc cacggcatgg ctgaggggcg ccttccggac gaaggggaga aaaacggcaa
     7021 atgaaccggc catgcgtgcc agtgcgacaa agctggcggg aagtccggac aggcaatttc
     7081 ccagcagtcc gaatgacaac gcccaaatga aagacgtgag cagcaacgca ggcatgcggg
     7141 cggaagcata cggagagagg aaaaccaagt caatgcccac ggcgcagagc atatagtcgg
     7201 gaacatgcaa aattacccaa aaaacggggt aagataatgg ttctaaacat aaaaacaccc
     7261 cgaaatattg aattccgggg tgttcaggaa aatggtacgc ggtactggtt tcgaaccagt
     7321 gaccccatgc gtgtgaagca tgtgctctac cactgagcta accgcgcatt tgcagtgcag
     7381 gaggaatata tcgttcgtgc cggggattgg caaggagttt tttgaaatcg ggtaaaaaag
     7441 gattccgctt ttccgccgtc aacaagttcc cggaggaatg accggtgtac agtttctccg
     7501 agggacggtt tggaaaagtg gaaaggtggc ttaattccgg cttgtacagc atcaaccgtc
     7561 ttcctacact ccgggcatga gttgcacaag ccctcccaga gcatgggccc agattgatac
     7621 caatgcgttg aggcacaatt taaacgtcgt ccggcgcgtc atgccggatc accgtctgat
     7681 ggctattgtg aaggcggagg cttatggcca cggtctggaa ggggtggtga aggccctgga
     7741 tggcgaagat tgcgcttttt tcggcgttgc cacggtggcg gaggcctgcc gggtgaggga
     7801 tgccggggca aaaacatgcc cgtttattct cggcccctgt tttgaggggg agcgggagga
     7861 aatcgtgcag aacggctggc gcgccgctct ttccagcatg gaagaggcgg agcatttcaa
     7921 ttccctgggc gccctttacg gcaaaaccgt ccatgtccat ttgggcgtgg ataccggcat
     7981 ggggcgcagc ggttttttac cgagtgaatt gcacggcctg acggacaagc tcaaacagtt
     8041 cagttatctg gatctggagg gcgtgttttc ccatttatcc gcagcttccg atgatatttc
     8101 cttcacccac ggacagatca gcggctttac ggaagccgta aatgaacttt cccaaggcca
     8161 tcggtacaag ttccggcacc tgtgcagcag cgccgctgtg tttaattaca aggttccctg
     8221 cgccaatatg gcgaggctgg gcagaatcct gtacgggtat tcccccatgc cgtctcccta
     8281 taataaggag ctgcggccta caatgaccct gtacagccgc attactctga tacgcacgct
     8341 gccgggaaat cacggcgttt cctacaacca ctgctatata acgaagggga gcacgaaggt
     8401 ggccactatc ggcatcggtt atgcggatgg ctatcttcaa tatctgtcca accagggagc
     8461 gagggtgtat atcaatggcc agtactgtcc ggttttgggc cgtgtgacga tggaccagat
     8521 catggtggac gttacttaca tggaccatgt ggagaccgga gacctggtgg aaatcatggg
     8581 gcctaatgta agctgggagg aactgacgcg ccgtgccaat accatcccca gcaatgtgct
     8641 gaccagcatc agcgcccgcg tgccccggat ttacgtgtag gataatgccg ggggcatggt
     8701 gattaaaagc tgttgcccgc cgcccgaaaa aaatcagggg atttgtttgc agatatgttt
     8761 ttccccggcg gacacggata aacgttttac cgtacctttt accgctggcg gattctattc
     8821 cagtcttccg gctccagagc gcagggatca ggggctaccc agtggcccgg ttctatttcc
     8881 acaggggtaa ggtccgctcc caccgtctcc ggataggagg ggtgctggat gcgatggccg
     8941 aaagcagagc cgagcggcgg gttgatggga gaggggacgt ctcccggcag aggctggtgc
     9001 ttgttggccc tggaggggtc tggttccgga atagcgtcca gaagagcctt ggtgtaagca
     9061 tgtttggggg attgatagat ggtttccgca tctgacattt ccacgatttt acccagatac
     9121 atgaccgcca cgcggtcgga gatgtgtttg acgaccgcca gatcatgggc aataaacagg
     9181 taagagagtt tgcgttcctc ctgaagttcc agcagcaggt tgagcacctg ggactggacg
     9241 gatacgtcaa gggcggagac gggttcgtcc agaataatga ggctgggatt taacgtcagg
     9301 gcgcgggcga tgccgatgcg ctggcgttgc ccgcctgaga attcaaaggg gaagcggtca
     9361 atagcgctgt ccggcaaccc tacgcgatcc agcaggccgc gcacccattc ccggcgcgcg
     9421 gcgggggtgc ccatgcggtg gatgacaaag ggttccgcta cgatttgacc gatggattgc
     9481 ctctggttga gggattccgc agggtcctgg aagaccatct ggatgtgcgg acgcagaggc
     9541 ctcattttgc gctgggagag tctggcgata ttgttgccgt tgaaccggat ggagcccccg
     9601 gtgggtttca gcaggcggac aatgcttttg ccaatggttg attttccgca gccggattcc
     9661 cctaccaggc ccagcgtttc ccccgggtaa atattgaagg aaacgtcgtc cacagccttg
     9721 acccatcccg cttgccgcag gaaaattccg gagcggacgg ggaagtacat tttcaaattg
     9781 tctacttgca agagaggttc cggcatggct ggtagaaaaa gaagttaaag gttggcgcaa
     9841 cgggggcagg cttccacgaa gtggtccggg gatatttcca cgaggggagg gcgttccgtg
     9901 agttcttccg cgggcacacc ctgcctttgg cagaagcggc atccgggaac aaaatccgca
     9961 atagaggcca cctgaccggg aatggtgggc agtttggttt tccgcacgga acttagctga
    10021 gggatggagg ccagcagtcc cttggtgtaa gcatggcggg ggttggcaaa aagttcccgg
    10081 acaggggcct tttccaccac gcggccagcg tacatgacga ctacggagtc acagctctgt
    10141 gcgatgacgc ccaggtcatg tgtaatgagg atggaggatg accccatttc cgcctgaagg
    10201 tctttgagca gggacaggat ttgagcctga acggtgacat ccagggccgt agtaggttca
    10261 tccgcaatga tgagttccgg atggcaggcc agggcaatgg ctatgacgac gcgctggcgc
    10321 atgccgccgg agagctgatg aggaaattcg tccacacgct gttccggggc ggggatacga
    10381 acgcgctgca ggagctggat ggctgcgtcc cgggcttccc tggtgttcat tccccggtgg
    10441 agcatcaggg attccccaat ttgcctgccg atgctgtgta cgggattgag cgccgtcatt
    10501 ggttcctgga aaatgacgcc gatatggcgg ccgcggatgc cgtgcatgtc cgtctctttt
    10561 gcctgaacca ggtcgcggcc cttgaacaga atttgcccgt ccaaaatttt ccccatgggc
    10621 tggggcagca gccggacgat ggacatggcc gtaacgcttt tgccgcagcc tgattcccct
    10681 acgatgccta cgcaggtacc tttggggacg gtgaagctga cgccgtccac cgctttcaac
    10741 aggccggatt ccgtttcgaa gccggtgacc aggttcttga tttccaacag gggatcgctc
    10801 atgtgttatt tttccgtggc agaaggttcc agagggtcgc cgctttccgg gatgacaggc
    10861 acagacggca gcttgccgcc gccatccttg ttatccaggc tttcaatgga gtccatgtag
    10921 cgatactggt catacacggt atccacttcc tcgaatgttt ttccttcccg cttggcttcc
    10981 attgtttcct ttttgatatc gttatccac
//
LOCUS       DAWEMX010000086        10793 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2574_length_10793_cov_10.391600, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000086 DAWEMX010000000
VERSION     DAWEMX010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2574_length_10793_cov_10.391600"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2464 a         3398 c         2691 g         2240 t
ORIGIN      
        1 gcccgttccc ccacaactct cccagctggt ttcccatgcc ctcccccgac cggccaattt
       61 ccccacacgt cccgtagcca tcccctcaag cgccccacgc acgccccgct gcgccagcgc
      121 ctgcgtcatg gacgccctta ccggcaaagt gctcttttcc cacaacggcc tccagcaccg
      181 ccaggtagcc agcacgcaga aactcgtgac ggccatggtc gtgatggaac acggcagcct
      241 ggacaaaaaa gtggtcatcc agccttccga taccaaggcg gaccccacca aactgggatt
      301 ccgggcgggg gaagtctatt cacgccgcga actgctcaat gccatgatga tccgcagttt
      361 caacgacgta gccctggctc tggcccgcga tacggcgggt tccgttcccc gtttcgccca
      421 gttgatgaat gccaaagccc ggcagatggg catgtacaac tcacgcttcg ccaatcccaa
      481 cggcctcccg gcggatcaat attccactgc cattgacatg gcccgctgcg cctattatgt
      541 gtaccgcaac ccggaactgc gcaatattat ctgcaaacgc caatatgcct ttacccgcgc
      601 caacggcaga acgcttctgc tgagaaacac caacaagctg ctcacccaga acccctgggt
      661 taccggaatg aaaaccggat acacgaacgc cgccggacgc tgcctggttt cctcagccgg
      721 cgtcaacggg cgccatgtca tcgtcgtcgt cctcggctgc catccgtccc gtatctggac
      781 ggaatcggaa aacctgatca gatgggcttt gggatccgcc gcataaatgc ccgtcggcaa
      841 ttggtatttg actttcacgg cgtgacccgc tatcagtcct gcctatgtta caggcaggta
      901 ttgtaggtct ccccaacgtc ggcaaatcca ctctattcaa tgcggtcacc cggacgcgca
      961 aggcccaggc ggccaactat cccttctgca ccattgaccc gaacgtgggt atggtaacag
     1021 tcccggatcc ccgcctgcag gttctttccg acatgtccgg ttctgaaaaa atcatcccca
     1081 cgcttattga attcgtggat attgccggtc tggtcaaggg agcctcggaa ggagccggcc
     1141 tgggcaacca gtttctggcg aacatccgtg aagtggacgc catcgtgcag gtagtccgct
     1201 gctttgacaa tgatgatatt atccatgaac tcggttccgt ggatcctctg cgcgacattg
     1261 acatcatcaa ctcggaactc attctggcgg atatcgccac catggaaaag agactctctt
     1321 cccgcgaacg caaggcacgc agcggcgaca aggaagccaa ggccgaagtg gccctcatca
     1381 ccaaactcct gccccacctg aacgaaggca atcccgccct cacccttgaa ccgcagctgg
     1441 acgacgacga acgcaagctg ctccattctt tccagctgct ttcggataaa aaaagcattt
     1501 tcgcctgcaa tgtcaatgaa gacgaactgg cggacgccat ctccaacccg gacgcccatc
     1561 cctacgtctc ccaggtgaaa aaatacgtag cggaacacca taacgcggaa gccattgtca
     1621 tctccgcccg gattgaagag gaactcatcg acgtctcgga ggaagaatcc cgcgaattcc
     1681 tggaatccct gggtgtgaag gactccggcg tttccgacct tatccgcgcc gtgtaccacc
     1741 ttctgggcct gcgcacctac ctcaccacgg gaatcaagga aacgcgggcc tggaccattc
     1801 cggccggggc aaaagcaccc caggcggccg gcgtcatcca cacggacttt gaacgcggtt
     1861 ttatcgccgc ggaggtagtg cactacgacg acctggtttc ctgcggcggc aaggccggag
     1921 cgagggaaca cggcaagctg cgcatcgaag gaaaagaata tgtcgtcaaa gacggggacg
     1981 tcatcgaatt ccgcttcaat gtctagacgg aagcgttacc ggaattccat tgacttttac
     2041 tgatataagg ggcagattgc tcataacctt cacatcgcct catgactgat acagacgtca
     2101 aatccacccc cctggcaact aaacacgttg aactgggagc tagaatggtt cctttcgccg
     2161 gctggaatat gccggtgcag tacaccggca ttctggacga acacaaagca gtgcgcgaaa
     2221 cctgcggcat ttttgacatc tcccacatgg ggcagttcac ggtagccggc ggttccgctg
     2281 cagcatggct gaactccatg ctgaccaatg acatcaacaa actgcatgta gggcaggggc
     2341 aatactccgt catgctcaat gacagggccg gagtcattga cgacctcatc ctgtaccgga
     2401 tggaaccgga aaccttcttc gtagtggtga acgcctccaa gattgatgaa gacttcgcat
     2461 ggctttccgc ccatcaacct gcaggcgtaa ccttggaaaa ccactccgac gaatacgtgg
     2521 ggctggctgt tcaaggtccc gaatgcggag aagtattctc ccgcgtcatc cccggcgtgg
     2581 aacttccccc ccgcaatgga atctcccgca tcacggcgga agggacggat ctcattatct
     2641 gccgcaccgg ctataccggg gaagatggtt ttgaattctt ctgcccggct aaggaaggcg
     2701 tcaaatggtt tgaagccttt cttggagcag gagccaaacc ctgcggcctg ggcgcccgcg
     2761 acagcctgcg tctggaaatg tgctatcctc tgaacggctc cgacctctct ccggacaaaa
     2821 cgcctctgga agccgggctc ggattcttct gcgcgctgga tacggacttc atcggttccg
     2881 atattctccg ggaacaaaaa gccaacggcc tcagccgtcg gctggccgct attgaataca
     2941 ccggcaaggg agctcctccc cgcgcccact atgccgtgca cgtccccggc ggagaagcca
     3001 tcggagaact taccagcggc gtgctctctc cttcgctgat gaagggcatt gccctggcct
     3061 acctgcctgc cgcctatgcc aaggtcggca cggagctgga aattgacgta aggggaagga
     3121 aattcccagc cgtggtcgta aaaaaaccct tttataaaaa aggctgacac tacggcgcaa
     3181 aaataaactg acaaacacct cattttacta cactctcact tcaaacaatt acatcttatg
     3241 cacgacgtac cagaaaatct gctgtattcc aaagatcacg aatggattga aattgacggg
     3301 gacattggca ccatcggcat ttccgaccat gcccaggccg aactctccga tgtcgtcttt
     3361 gtagaccttc ccgaagtagg cgccaccgtc gcagccggcg accctgtcgc tgtcgtggaa
     3421 tctgtcaaag ccgccagcga cgtgtacacc cccgtttcag gagaaatcct ggaagtgaac
     3481 gaagagcttt ccaatgatcc gtccctcatt aactccgacc cctacggcgc aggctggctg
     3541 tacaaaatcc gcctggacgt tcccacggaa acggaagata tgatgaacgc cacggattac
     3601 gaggaatact gctcctgaac cattcttccc tcccccgccc cggtgattcc atcccggggc
     3661 attttgtttg gcccgttccg cggccggacg ctattaccag ccagagatac cacatgaata
     3721 cccactcaga ttttgccagc cggcacatcg gcccccaggg ggaagaacgc cgggaaatgc
     3781 ttgacagcct cggctaccgg acgcttgacg aactcattgc ggacatcgtc cccgcagata
     3841 tccgcatgaa ggccccgctg gacctgcccg cagccaaatc ggaaacggaa gccctggagg
     3901 aactccgttc cattctgcgg aaaaacaagc tcctgaaaac cttcattggc cagggctatt
     3961 acggcaccat cactccctcc gtcatcctcc gcaatgtgct ggagaacccc ggctggtaca
     4021 cggcctacac cccttatcaa ccggaaatcg cccagggacg gctggaaatg ctcatgaatt
     4081 tccagaccat ggtctcttcc ctgacaggac tgccggtagc gaacgcctcc ctgctggatg
     4141 aaggcaccgc agcagcagaa gccgtcacca tgtgccggaa cgcgcgcccc aaagcaaaca
     4201 ccttctttgt ggcggacacc tgccaccccc aaaccatcag cgtcatccgt acccgcgccg
     4261 ccttccaggg cgttaacatc atcgtgggag attgctcctc ctttgacccc gcatccatcg
     4321 gtgcggatct ggccggagtg cttgtccaat atccggacac gctcggccgc atctgcgact
     4381 acacggactt ctttgcccgc gtgcatgcca cgggcgcgct ctgcgtcgta gcggcggatc
     4441 tcatggccct taccgtcatc cgggaacccg gcgccttcgg aaccgatatc tgcatcggca
     4501 acacgcagcg cttcggcatc ccaatgggct tcggcggccc ccatgccgcc tacatgtcct
     4561 gcacggacgc gctcaaacgc cgcatgcccg gccgccttat cggcatgtcc atagatacct
     4621 tggggcgccc ggcctaccgc ctggccctgc aaacgaggga acagcatatt cgccgcgaca
     4681 aggcgacctc caacatctgc accgcccagg tgctcctggc cgtgcttgcc gccttttacg
     4741 ccgtatacca cggccaggaa ggcctcaaac gcatcggcac ggaaatccac ttgaaaacca
     4801 aaagcctgta caaggagctc acggaagccg gcatcgccat tgaaaacaaa aacttctttg
     4861 acaccctgct gctgtccgtt cccggacaag cggacgccat ggtgcaaaaa gccctggaag
     4921 ccgggtataa catccgcagg gtggatgccg accacgcagc catctctctg gatgaaaccg
     4981 caacctgtgc ggatatagcg gcactggcct ccgcccttgc aggtgcggaa acatccgccg
     5041 cctgcgattg cgacgctcct gcctgggatc ccgtccacac gcgccagacg cctttctgca
     5101 cggaaaaggc tttcaactcc taccattccg aaacggaaat gatgcgctac atccgccgcc
     5161 tggaatcccg ggacctggct ctaaatgaag ccatgatccc tctgggctcc tgcacgatga
     5221 agctgaatgc cgcctctgaa atgattccca tcacgtggcc ggaagccaac tccctgcatc
     5281 cctttgttcc ggcggaccag tcggaaggca tccgggaaat gctctccatc ctgtccgacc
     5341 gtctggcgaa aatcaccggc tttgccgccg tctccctcca gcccaacgcg ggcgccgcag
     5401 gggaatatgc cgggctattg gccatccgcc gttaccagaa gcacgccgga gaaggtcacc
     5461 gcaatgtctg cctcattccc acctccgccc acggaacaaa tcccgcctcc tccgccatgg
     5521 ccggcctcaa ggtggtgccc gtcaaatgcg acgaaagagg caacatcgac atggcagacc
     5581 tgaaatccca ggcggaagcc cataaggaca atctttcctg catcatggtc acgtacccct
     5641 ccacgcacgg ggtatatgaa cagacaatca aggaactctg cgacatcgtc catgccaatg
     5701 gcggccaggt gtacatggac ggcgccaaca tgaatgccca ggtaggtctg acttgccccg
     5761 gctgcattgg cgcggacgtc tgccacctga acctgcataa aacctttgcc atgccgcacg
     5821 gcggcggcgg tcccggcatc gggcccatcg gcgtagcgga acacctcgta cccttcctgc
     5881 ccgggcacct cacgctgggc catgaagaag gggccgtagc gtctgcggcg tggggaagcg
     5941 cctccatcgc cgccatctgc tggatgtacc tctccatgat ggggccggac ggcctcaggg
     6001 aagccacgga aatggccatc cttaacgcca actacattgc caagaaactc ggacatctct
     6061 tcccggttct ctactccggc aacaaggggc tggtggcgca tgaatgcatt ctggatccgc
     6121 gtcagctcac tcatgacgcc ggcctcacgg tggatgacat tgccaaaagg ctcatggact
     6181 acggcttcca cggccccact atgtccttcc ccgttccggg caccctgatg gtggagccga
     6241 cggaatcgga acccaaaaaa gaactggacc gcttcattga agccatggaa cgcattcatg
     6301 cggaaatcac ggccattatc aacggtacgg cagacaagga agacaacgtc ctgaaaaact
     6361 cgccccatac cgcagaaatg gtctccgccg acgaatggcg gcacccctac tcccgcagtg
     6421 aagcggctta tccggtctcc ggtctcctta ttcacaaatt ctggccgtat gtaggccggg
     6481 tggataatgt gtacggagac cgcaatctgg tctgcacctg cgacacggtg gaggaattct
     6541 ccaaggccgt agagttataa aaatctcatc cttcctttca aaaaggctgt tccattcatt
     6601 tggagcagcc tttttcttat gttcaagaat cggatttcga atccgaagaa acaaacgcat
     6661 ccagaaaaca attaagcata aaagcagtat gtattaaaaa agaaaaaggc acatttctcc
     6721 caccccgctt ttcctcccct ttcattttga tgacccattt gtactcaaaa tagagtgttg
     6781 acttcggatt cgaaatccgt tgagctacgt ctcagatgag ctttttctct gcccttactg
     6841 ttcctggcct tcaacagcat ctttcttttt ccagggaaga ttccgctatc ctcaaagggt
     6901 atggaattct cttcatgatc tttcatcatg tcttcggctt atacgctctg cccgcaggtg
     6961 tggatacggc atggattgct ccccagctta ccaaagccgc tcccatcttt aaaatttgcg
     7021 tgcccatttt tattttcatc acggggtacg ctatgggatg gaaaacaaat tcatccccaa
     7081 ccttcggttc cctcattaaa acaggctttt cccattactt taaattctgg aaaatttatt
     7141 tcttatgcct ccttctggct attctggttt cctgggcatt ccccttgccc attttgccat
     7201 ccgttgccga catgggttgg aaaaatggcc tgcttgtcgt aacaggattg aggccctgtt
     7261 atcccgactg gtggtacatg gctcttttcg cggcagccac catggccctt tatccaatct
     7321 gtgcgtggat cacgcatcac attgcgcctg tcccgtccat ggcagctctg ctgggagtgt
     7381 cccttttatt tcaaagtacg gcccacattc ccagcctgcc gggaatcgcc tcatccttcc
     7441 ctcctttcct gccctgcttc attctcggct acatgtgcgc ctttctggct tcacgcctct
     7501 ctgctctttc aatttcccac agcttgggag caatcctgtt gctggctctt gaaatactat
     7561 ccatccatct cttcagtttt tcaaaagcca aaaccctgac ggttatcttt ctcttcaccc
     7621 tctggtgtct gccttggata acccggaaac tccgcctcac accgctcttg acgctgctgg
     7681 gaacttattc cgcacttatg tggctgaatc acagattcat tttcggctac cacttcagct
     7741 gggacctcta tggaacccaa agcatcagcg tagtattcat cgtgacgctg gtctcctccc
     7801 ttctgctggc catggccatg caaaaacttt ttaaccggat gattccctca aatccgcaaa
     7861 cgcagtcaaa aactttgtag caactttaca aggtccggat tccaacccct cttaattcca
     7921 agggggatct ccgtacttat tctctccaat ccggctttcc ccatgggata aataccacaa
     7981 cagcagccac cctatgaagg gaacggccag aaaaaccagg aaaaagccgc tcctcccgac
     8041 atcatgcaga cgccggacca tggtagccgc cagcggaata gacagcacgg caagcaccac
     8101 gtacagcaga ggggaaacca ggtcaaccca cggaaccagg gacgggaaaa aaccgcattt
     8161 tacaaaagag ggggctcccg ccacggccaa ccacgcaacc cccactccgg aaaaaacctc
     8221 gtgcagctcc cctttcccgg cacggccctc aaaacggcac catgccttga tgacgcgcat
     8281 ccagtacttc ttcctgctcc agccggagtc ggttcctcca ggctccggtc cggggtccgg
     8341 cagaacggag ggctccccat catcatccat gacagtcatg gaggggataa aagaggccag
     8401 aggcctccag tctccgccgg gaaccttccg gaccaaagtc cggttggaca ggcggccgag
     8461 gttacgggcc cgcaacaaga gcaccaggtc aaccgggcct ttggaagaac cgtcaattgc
     8521 ctggtactcg tacagatcgc tcatagggac gcttacctgg attccttccc gtgactgaag
     8581 gcggaggcgg gaatctcctg cttggacaaa ccggggcctt cccacttcaa aaccagtttg
     8641 gaggcagtac cggagttccc cacatagccg atgcggatgg gatgaagtcc ggctttcaaa
     8701 taaacgggat tcttccggtc ccccaaatcg gaggagacct cggcccccgg ctcataagtt
     8761 ttgtccgcgt caatcagttc catgccgtgc agacggacaa aagccttgct gcccttattt
     8821 tcatccgtgg tcagataaaa cgtatatgct ccgtcctcag gaatcttcac atatcccgtc
     8881 agctccacac cgcgcttggc cggaccgttc attttcacgg atggggaagg cgtcacgcca
     8941 tgggcggaag caggcttctt ccactggcga aaatccggta cccagggaaa atccccctca
     9001 tacaaggccc attgcaaccc gggagaaacg gttcccttca catccacggc aggcaccagc
     9061 gcagaatcaa acactgtttt ggaaggaagg gaagcccggc gattggacaa agcggcggct
     9121 ttcatgcggg cctgaagttc cggcttggag gatgcgagat ctttgctttc ctgcggatca
     9181 ttcaaagtat caaaaatcat gaaatccttg tccgcatcct tcacccccat gcgcaggccc
     9241 ttcaatccat ccacaaagac aatctgctgc tggcctcgct gcgctccctt gtgttcgccc
     9301 aggaaatcct tatactccgg cgttttgccg gcgaaattat attcggcata aacaatgccg
     9361 ggcttctgct ggtccgcatg accggtcagc gtcggcagca gggaaacgcc gtcactgcgg
     9421 gcaggaaccg gcaccccggc gacatccgcc agcgtagcca gccagtcatg gaactgtcca
     9481 agatgcaggc tgacctgccc tttgggaata cgggcgggcc agcgtaccag cgtaggcacg
     9541 cgcatgccac cttcccagca atcgcgctta atgccgtcca tcatgccata actcttgaaa
     9601 aactgcggat tttgcgctcc gtgccagtgc ctggagtcgg aaccgccctc attgtggggg
     9661 ccattgtcgg acgtgaagac gatcatcgta ttatcgtcaa tcttcaaatc tttcagcaga
     9721 cggatcaaat cagctacggc gtcgtccacg cggcggatca tggtggaatg acgtttggcc
     9781 acatcattcg ggaaacggga attgtccgga tgaatatagg tatccttatt cttttccgcc
     9841 ctggcgtcaa aagccgtatt cacggattcc gtgccttcct tctttaccca ttgcaggcct
     9901 cctttcaacc cgccgccgga aggatagggg acccccggaa ccaccagatt gccgtggggc
     9961 gccggaaaag ccaggtacag gaaaaacggc tttccggtct tgcgggcgga cttgcgctgg
    10021 tccacaatcc actgcttggc gcgggcggca aataaatccg tggaataagc cgtctggggc
    10081 acctggtcct tgatattttt ccactcggga ttggaagcgt aaccgttgta ctcgaaaacg
    10141 tcacgggatt cggaaggata atggaaatgc cctgccaaat ggtccaggat accataaaaa
    10201 taattgaatc cccgctgatg ggggccggcg gtcatgggca catggctctg gcctccgccg
    10261 cctacacccc atttgccaat ggcggcagta tcatacccgg catcacgcat cacggtaccc
    10321 agcgtatggg aatcttcaat aggctggtcg aaacggttgt ttctcaccac gcgggaattc
    10381 ccctggtgca cccccagcag cagggaggcg cgcgccggag cgcaaaccgg agcggcggaa
    10441 taatgacggc gcagctgaat cccctcccgg gccagagcgg acagggcagg agtcttgaac
    10501 tcgttctttc tctccaccgt ccggccattc agcttctgct ggctccagtt ggaatccaga
    10561 tcaccccagc ccatgtcgtc caccagaatg aaaataacat tgggttttga agttttgaca
    10621 gaccgggcag ccggcttcgc tgcctgcccg gaagcgccca tgcacggaac cagggaagtc
    10681 agaagaacgg atagtaaaga taccagcttc atgattcaaa gaaaagagac tattaaactg
    10741 acgttaaaac aaagcgtcat cttacatgaa ttttctcatc tctcaagaaa att
//
LOCUS       DAWEMX010000087        10550 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2657_length_10550_cov_10.157504, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000087 DAWEMX010000000
VERSION     DAWEMX010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2657_length_10550_cov_10.157504"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3522..3531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6487..6496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2512 a         3313 c         2671 g         2034 t
ORIGIN      
        1 gctcacataa gttttaccga cttccgtatc ggtaccggta acaatgaaat tcctcataac
       61 ggtaaatatt cagggatggg atcttctaat ctcctcaagc tgcccgcggg aaggcacgcc
      121 gtcattcgtg gtcacccagt gattgatgaa agaccagata atcacggcca gcaccagaca
      181 gaacaacttg gcaggccaat taagcaatag cagttttttc attggaagaa gaaatattca
      241 gcagctcttc aaggcgtccc ttcaactgtt ccggagtcag gttgcgttcc agcttcccgc
      301 ctacggccaa cgcgatagcc cccgtctcct cggacaccac caccaccacg cagtcgctct
      361 cctcggacat gcccacgccc gcgcggtggc gcagccccaa ggtacggtcg ctcatttcct
      421 tctgggaaac agggaacacg caggcagccg cagaaatgcg gtcgccggaa atcaccacgc
      481 ccccatcgtg gagagccgtc ttggtatgga aaatcgtcag ggccagttcc ggagaaaaaa
      541 tggcgtccag cttcacgccg gaatcctcaa taggcttcat gctgatgctc ctctcaaaag
      601 cgaacagggc gccgaaccgc tggttggaaa gcttgcttac cgccttgcag aaattatcca
      661 ggaaatccac ccgctgaagc ttggcgaagg aagaaaaaaa cgggtggctt cccagctttg
      721 ccagccccac gcgcagctcc ggctggaaaa tcaccaccag agccagcgcc aggccggggg
      781 cgaaaatgcg cgtcagtatc cacgaaataa cattcagctg aaagaaaaag gccagcacca
      841 ccagggccag aaagcaggcg aacagcccca ccatgatgcg cgcgccgcgc gttgcgtgga
      901 aagcacggta aatctggtac aggaacaccc acaggatgaa aatttcaaca accgcccgga
      961 gcgcatcctt gatcacaatc cagtcccaca tgcccaacag gctacacaac aacccggccg
     1021 aatgcaatac cgatcctctc gcgttgccgg caggctccgg gaatttaccg ttcatctcag
     1081 ttcccgaatt tgaagacatc tctgtacgtt ccggagacag atagaagttc ttaaaaagaa
     1141 gagcagttct ttggaaaata aataataaca ataaataaaa attggattaa ataaacaatg
     1201 ttaatccgca taaagcaagg cttcagaaaa ctgaaattct ctctggattt acggcatgaa
     1261 cttgcggcgg atatactgaa aaaccaaaca aaaaataatc accagatgat agcaatcgaa
     1321 caattaaaag aagtagggaa aatctggctc gcctgggtaa ggcttcagga tcatgatcct
     1381 gccgaagtca cgcaaatatt tgaaaaactt aaaggagaac tggatgtaaa attaggaaga
     1441 ggctggcaaa aatatgaccc ggtaataaaa aaaggaaggc cccaatccta taaaaaactc
     1501 tttcaagaat tggatagtga cagtcaggaa tccgcaaatg cattgatcaa atatttcaat
     1561 gagggaatac cggaagacgc tcctgtctcg ccagtcatac acctcattct catcttatgc
     1621 gtctgtgaag tgggacgtgg atatggccag gcgccggaca atatgctggc ttatgcacat
     1681 gatctcattg attatcccaa tctcacatgg ggctcactgc tggacacagc gggaagttcc
     1741 ccgaagttcg catttctatg caagggtgat tccgatgaag actataagga ataatgcatg
     1801 agcgccgtcc gcaaccgact caccggtgag cctcccgcac ggcatgggtt ccctgtatcc
     1861 gggcacaggg aacttcaccc ccatcgtcgg caaaaaggac agccgcaggc gggctcgggc
     1921 aattcagaaa cataggcggc ccttcctgcc ttccatctgt ctcaatcctg cggacatcaa
     1981 aaattggtgc atcaactttt ctcatcacct caatttccgg gccaaaaacc tggccgccgt
     2041 catgataacg gacaccttcc ttttccggaa accgaactgc catcaacagg aaaccttccg
     2101 gaaacgctgg attaccgccg catggggcag atgtcccaga gcggcaccag gccgtcctgc
     2161 atctgacggc ggacatcaaa taaaacgcag gcggccccta cgcccggcag cctcagacgg
     2221 cctgcccgtc cataatcgta ataatgcggt cccctctccg tgccagatgt tcgtcatgcg
     2281 tcacaatcac cagcgttttg cgggactcgt ccgccaggcc gaacaaaagg tccagcacct
     2341 ccgcgccgtt cttccggtcc aggttcccgg taggttcatc cgcaaaaata atgggggcgt
     2401 cgttcgccag ggcacgggcg attgccacgc gctgctgttc cccgccgctc agttcgctgg
     2461 gcaggtggtt caggcggtgg gacaaaccca cgcgctccat cagttccctc acatattcca
     2521 cgcgcggcct cctgccgatg gaggaagcca gacaggcgtt ttccagcgcc gtcaattccg
     2581 gcatcaacag ataattctgg aaaataaacc ccatgctccg gttccggaac acggaccggg
     2641 ccgtggcgga caaggcgtaa atatccgtgc cgtcaatcgt gacctttccc tcttgcggcg
     2701 tttccaagcc ggccagcgtg tacatcagcg tggtctttcc ggccccgctg ggcccgcaca
     2761 ggaacacctt ctctccggcg gcaatgtgca aatccacccc atgcaggatc tcaatggact
     2821 tcttgccgat ggaatagctg cggtggaggt tggaggcggt aatcacgggc gcagtataag
     2881 aaaatccttg ccgtattaaa gttctatgtt gtctataaga cgcacgtcgc cgaaatagac
     2941 ggcggcggcc atcagggccg gcttccggtt ctccgccagc ggctgcatgg tctccgcatc
     3001 cacaatctcc agataatcga tgcgcgtgcc gggcacaccc tcaatcatgg cggcggcatg
     3061 ctccttcacc ttggaggcgt ccgccccggc ctggaactcg tcccgggcct gaagcatagc
     3121 ctgccgtatt accacggcct gttccttctg ctccggcgtc aggcgggcgt tccgggagga
     3181 ataggccaga ccgttgccat gccttacaat ctccgccccg tgaatgcgca ccggaaaatc
     3241 caggtcgcgc accatgcggc ggataatggc aagctgctgg taatccttct ttccgaaaat
     3301 ggcgtccgtg ggctgtacca ggttgaacag tttgacaagc accgtgcaga caccggaaaa
     3361 atgccccggg cgagacgcac cgcacaatgt tgcggacaaa aagctttctt ccacggcaat
     3421 gctgcgttct ccggaataca tttcttcagg agcaggggaa aacacataat ccacgcctgc
     3481 accttcgcac acctccaaat ccttttcagg agtccgggga tnnnnnnnnn ncggtggtgc
     3541 ttgaggaggg cagccctcaa ctgggccttg gtggaaaatg tttgcatgcg cctaaagtgg
     3601 ccccttgatg acaaaagttc aatcctgaaa tattgctaga aggttgacgc atgccattcc
     3661 acatgtaaac tggtaaagtc agaagacgct aaaaattatg agattcttca aaacagcatt
     3721 aggtgtatca gcagcaatgg cattaagcgc cacggccaac gctgaactta aggtagcgac
     3781 ggtggatgtg cagaaactct tcgccgacta ctacaagacc catgaagccc aggcggaagt
     3841 agacaaggct gcccagacgg tgcaggaaac caacaacacc cgcgcggaaa ccatcaaaaa
     3901 gatggaagcg gacttcaacg acatggtcaa aaggcttcag gatcccatgc tgaatgaaaa
     3961 ggacaaaaag gagctggagc aaaaggccca gatcaagcgt caggaagtca ttgcgctgga
     4021 acaggaacgc cgcggatttg tggaacgcca gcttaaatcc cttcaggaac aaatgaaagt
     4081 gcgttccgcc aaaatcatgg gagaaatcac aaaaatcacg gaaggcatcg ccaccaaggg
     4141 caactatgac ctcgttctgg acaaaagcgc ccaggccctt cggtccaacc aggtattcgt
     4201 ctacacgaag cccggcatgg acattactcc cacagtgatg aaagaactca acaaagacgc
     4261 ccccgaaggg tttgacccct ccaagaaaaa ggctccggcc gtccccgccg ctcccgcggc
     4321 cccggccaac taacccttga cattaacccc gggaaggcgg aatcatatac ccatgaagct
     4381 tacacttgaa gccgtggccg ccctcaccgg gggaaaaatc ctttccggcg accccgaact
     4441 gaccgtattc ggggtcgctt cccttcttga cgcatcccgt gaagaagcct ccttccttgg
     4501 caatgaaaaa tacttccagg acttcctgca cacctctgcc ggaatcgtgc tggtgccgcc
     4561 gggcctgcct gcgtatccgg aacacgcagc cttcgtggaa gtggacaacc cctccatggc
     4621 attcaacgcc ctggtgaaat atttcatggc ttccgcctac cggttcaccc ccggcattca
     4681 tcccacagcc atcatcgacc ccacggccag cttcaacccg gacaaaatcc atgtaggagc
     4741 ctatacctgc atcggggccc actgcataat cggagacggg acggacattg gcaacggctg
     4801 cgatatcgga gacggcgtca ccatggggga aaactgccgc ctgcatgccc atgttaccat
     4861 cagggaacgc tgcaaactcg gcaacagggt gaccatccag cccggagccg tcatcggttc
     4921 cgacggattc ggcttcctga tgggagacaa tggacgctac gtgggcattg accaggtagg
     4981 cattgtggaa ctgggggatg acgtggacgt aggggccaac accactattg accgcgccag
     5041 attcgggcgc accattgtgg gggaaggcac caaaatagac aacctgatcc agctcggcca
     5101 taacgtcgtt gtcggcaggc attgcatcat cgtcgcccaa tccggcatag ccggcagcac
     5161 caaagtgggg gattatgcca ccatcgccgc ccaggtcggc atctcaggac acctcaacat
     5221 cggttccaaa tccacgctgg gcgctaaaac gggagtcctc tcggacattc cggaaaactc
     5281 tacttactgg ggcatgccgg ctttccccta caaggacgct acccgccaat acgccgccct
     5341 caaaaaactt ccggcactga ttaaggaagt ccgcgcgctc aaaaaggagc tggattcttc
     5401 cggcaaataa cgggcaccat atcgccggaa cgctctgcag cccctctccg tgtttctgtt
     5461 tcccgccgct tcaggcctgt cctgtccccc agcggcggtg cagaaaccgt tccagaggag
     5521 aaaacagaat cttgtcggac agtagcccaa tcaccacaat cacgaacatg atgcctacga
     5581 cctggttcat cgcgttcagt tcgcggccga aatgcagcag ctggcccaat ccgaacccgg
     5641 tcaggatggg aacaaaaatt tccgcagcca tcagggagcg ccaggcaaaa gcccatccct
     5701 gcttcattcc gctcaccaca aacggggcgg aagcgggaag cgtcacatga atcaggcaat
     5761 aaatgggtcc tgctcccatg gtgcgcgccg cacgggcata aatgggcggc acgttccgca
     5821 tgccgttggc cgtagccagg attacggacc acagcgtccc cataatcacc acgaacagca
     5881 tagccccttc cgtctggccg aaccacaggg ttgccaacgg aacccagcac acgctgggca
     5941 gagcctggaa ccccagggaa accagcccca gagtattctg cataagctgg aaacgggagc
     6001 acagcatgcc caggggaatc cccgtcacca ggccgattcc ataccccagc accagccttt
     6061 tcatggtaat ccatgaagcc tcctccagag acccgtcctg aaatgaagag cgcagatact
     6121 cccatacttc cagcggagag ggaaacaaat aaggcttcca gaccctcagg tcgctcaaat
     6181 actgccatgc ccagaccacg agaatgaaaa acatgagcga acaggcgtgc gccccccact
     6241 tggaccattt gttcctgttc atgctgtggc tcctttcaaa tcacgggtga tgcgggcgga
     6301 caatgccgcc aaatcactgc tgttgatatc cctgggatga gggaaatcca cgaaatattc
     6361 ctcacagacg cgccccggat gcggagtgag caacaccacg cgatccccca ggcacacggc
     6421 ctcgcgcata ttatgcgtaa cgaaaacaat cgtcatgccg cagcgtaaat gaatctcctg
     6481 aatatcnnnn nnnnnnctga taaagctgtt cgcgggtcat ggcgtccagg gcggcaaaag
     6541 gctcgtccat cagcaaaatg cgggggctgg tagccagggc gcgggcaagc gccacgcgct
     6601 gcttcattcc gccggaaagt tcatgaatat gagcatgggc gcactctttc agccccacca
     6661 gttccagatt cttctccgcc atggcaagcc ggtctccccg agtcagtccg ggcaccagat
     6721 tcaagccgaa catcacgttc cccatcacat ccagccacgg aaacagggcg gactcctgga
     6781 acatcacagt tcggtcccgt ccgggtcccg tcacaggctc cccgtccagc tccaccaccc
     6841 cggaagaagg aaactccagc cctgcaatga tattcagcag ggaagtcttc ccgcagccgc
     6901 tggcccccac caggcacacg aactcgcctc ctctgatatt caaattaatg ccctccagag
     6961 ccaccacctt tccgcgcctc ccgtcaaaca ccttgctcac gccggcaata cggagcttgc
     7021 agcctccggg gccgcaatgt tctgcacccg caatcatctt ctgcccgcct ccttcgccat
     7081 ggtcaactgt tcctccgccg cctccggcac caccaagccg cctatatccg gaacctcttt
     7141 cataaatccg gcctgatggg cgtcctgcac aaactgccgg agcttgggaa tggaaatcct
     7201 gtccttgatg acaatacttt tccaggcctc cgcaatcaat gccgggtcta ttctggaatg
     7261 cgtcagctcc tccagctccc tgaccacgat tgactgggcc tcatccggat gcagccttat
     7321 ccattcgttc agttcctcat gcgcctgcac cagggctttc gcaacctccg gcttttccct
     7381 gagaaactcc gccccgcata ccagtacggt cgcgatggac tccttctcgt ccaccagcac
     7441 cctgcctgcc tccgtcagca ccaggcggct cacccagggt tccaccgtcc acacgccgtc
     7501 cagcttgccc tgccggaaca ggctgagctg ttcggggttg ggagtcggca gaatcgtcac
     7561 gtccccgccg cgctgcgtca catgcaggcc gccgcgggaa aaccaggcgc gggcggaaac
     7621 atcctgcgta ttccccagtt ggggagtagc aatcaccttg ccgcggaaat ccgcaggctc
     7681 cctcagcgtg gaatccttcg gaaccaccag ggcggcgcct ccttccacag ctccggcaat
     7741 catcctgata tcctccccgc gggaacgcac aaacgcattg atggccgggc tgggccccac
     7801 ataagccagt tcaatggacc gggcgaacac ggcttccatg gcgctggggc ccgcattgta
     7861 cacgtaccag ttgatcctga catcctttcc ggtagcctcc ttcagccgtt cttcaaacca
     7921 ccccttcccc tgccgggaaa aatgatgcgc caccaggccc tgcacatgtg tcacgttggg
     7981 aaaatgaccg aagttcaact ccacgacatt gggatcttcc ttctccttct ttccgcagga
     8041 aacagccagc aggcataaaa acagggccgg taaaatcatc aggaatctgg aacgggtcat
     8101 aagcaaaaag aatgggatac tgaggcacag ccgtttttcc gcggcctttc aggcaccgac
     8161 tagcagaaga agccgccgga ggcaagaagc caaaggcaac aactttgtca caatgtggac
     8221 agcaaaaatg cccgttttgt tgaaaaacaa tcccggttcc ggatctgaac cgggctttat
     8281 tgacaaaccc gcacgttccc gttacaaaag caccattccc aacccgccat gcaatcctcc
     8341 cccgaccagc cctcatccgg agcatggctg ggccttcctc cggatgaaga acgcccggta
     8401 agccggggcg ccaggtaccg ttgcatcttt ctgatgctcc tgtactgggc cgttctttcc
     8461 gccattccac aaggcgcgga ccgccttgca gaaataggaa caaatctccc tctctggatg
     8521 ggagccctgg aactgactcc cttcctgctg gtgccggcct tcctgttcct ggcggtgcga
     8581 acaggcgccc aatggagggc cttgatgata accgtatatg cgggcctgtt cctgaacctg
     8641 ctgatgaaca tggcgcttcc ctcaggcgcc atgtggggag accttcccgc agccggcccc
     8701 tacggtacgc ttgtctggcc cggaggggac caccgggaat ggctggtcac gctgccccgt
     8761 tttccccaat tctgggctct cgtcatctat gtcttcctct ccggcagcgg aaccccgccc
     8821 ctgcttcgcc tgctggccct ggcctggtgg ttcctgcttt gcgtggcccc cgtgaacacg
     8881 ggcatggtgg ggtatgcgga catcctaggg cctctggtca tcatcgccct cgtcctcggc
     8941 tgcatgcacc tggcggaaaa aagagcctcc cggcaaacca agggctaaaa atccagggcg
     9001 gccattccac ggaagaaaca gccgccccag ggccagcgga caccacccgg cggaaaactt
     9061 cccgatgcca tccgttattt ccagattact tcaaaaatat ttacggaact tccctttttc
     9121 ccggtcaggc cgagggcctt caccggtttt ttgacggatt tcaggctgaa cgtattaact
     9181 ttcctgccct tggccagatt gcccacggga acccaccgtc cgtcagcccc acaggccagc
     9241 acggaagcct ccttcccgtc ggacaggaca atgacggaac ccgcacgggg aaaatcacag
     9301 ggaatctccg ccctctccgg agacagctcc acctggagga acgttgccag gttgaaatcc
     9361 ccggcagctc cgctgtcggc ggacttccca cccttgttgg caagattcag ccggaactct
     9421 tccagagtca cctcctgcgg ggaggaggag gcgttcaaca gcctgatgcc cttgatcact
     9481 ccgccaatat ccgcggaacc gtcctccttc aacgaaacct ccgcccagga ctttccatcc
     9541 atggaaatga acaacttggc ccacttcata tccggcgtct tcaaattgac ggaggcggac
     9601 tccacggacg ctcccgccgg aatttgaagg cccagggact ttttcggcgg caggggatgc
     9661 acctccatga tgcgcttcaa ggaaatatcc ctgtctcctg cttcaaccac ggcattcctg
     9721 aacgcgggag catccgtcag tacggaaggc gctgcaggag cattcacctt gaaatcccgg
     9781 atggccgtcc acacgtccgg cttgcctccc ggaaccccct gcgccgtagc gcggtaacgc
     9841 acaaaccggc cttttttgcc atttccccgg tactccacgc gaagcccgct ggactccggc
     9901 atcaggggag accaggactg cccgtccagg gaaacctcca actgtcccct gttcacagcg
     9961 tccgtatcgg aatcattgcg gcccatcaca atggaaacgg tgcgtatctc ccgtggaact
    10021 cccaggtcca cgccgaaaaa gtcacccttc ttctgaacct ctttgcagta atagaacgac
    10081 tcggggtcgt catccagcat cttctcgatg ccaatcttgg acttggtgga aacataaggc
    10141 ttcacacggc caacggcctg cccgctcacg cgggaaagca cgggggtgga gcccatgtcc
    10201 agcagttccc gcacagcggg ggccatcacc agttcggacg gcttcacggc ggtttgccac
    10261 ggggaattct tcttggtcac ttccgtgacg tgcttattga tggccttgct gatttccctg
    10321 ctgtaacgct gcatctcggc aagcaggcag gcggcctcca tagcatgatt caacgctccg
    10381 gcggcattcc ccacctctgt ggcctcaatc atgcgcatac tgttcacccc ggctttcccc
    10441 agggattcga actggatgag ccacggctcc acctctgcaa cgaaagcggg attattagac
    10501 ttggcccgaa tcgcggccgg agcttgggct atcttcgcga actcggcctt
//
LOCUS       DAWEMX010000088        10526 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2668_length_10526_cov_10.597842, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000088 DAWEMX010000000
VERSION     DAWEMX010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_10526_cov_10.597842"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    636..645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2601..2610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2376 a         3192 c         2665 g         2273 t
ORIGIN      
        1 aacaaaaata caaacaaaaa gaattaacta catgataaca caatctatac ccaacaaaaa
       61 acatcttcaa aaaaccctag aaaatataaa agtatacttt aaaacaattt aaaaaggaaa
      121 tttctgaaga aaaaaagtaa taaatagcat tgttcaagag ccaggaactg gttaataaag
      181 gtagtctgct tctgtacctc cttcatattt tggtctcttg cttgcagtgt gtaagaggct
      241 cttcaaaatt accctattta ccctgtgcga tatcattcaa ctcacacaaa ttcaatgtat
      301 tgaatgacac aaagtaaaaa atgcgcaccc tgtcttgccc tgtatcccac cacgtagcgg
      361 tccattttat caccacaaaa taaactgatt aagaatattt ccgcactctg ttctaccctg
      421 tcacggaatg ggcaatggtg tacacgttcc tccgtccgcc tccttccttt cgtacctgca
      481 cgcgatccgg acacatctct gccagtttgg ataactttcc gcccagttta gccgcgttcc
      541 tggccagaag attgaatgct ctcattttgt cagcaggaag attagctctt acacggtcat
      601 aaatacccgt ggaagtggcg tccttcctgt agatcnnnnn nnnnntgcgg cctggtcaat
      661 cagttccagt aactgcattt ccgggctgat gcccagaaca gccgtggcta gtgtcttgtt
      721 catgtatgct ctgcatcggt gacgtcccgt aggttcctgc aaatgctccg gcgcccggta
      781 atggtcaatg aaccacgcca gagcgggcaa ctcccttgcc agggcctcat ccacggcatc
      841 cttttcatct tccgtcccat tcggaggcgc ctcatgataa ccgctcagcc agaaagccgc
      901 atattttatc tgtatcgcct ccctccatcg gaaaagccgt caggctctct ccggatccgt
      961 gttgagcaac tgcaccaccg cccaggacaa cgccatcggg aaggcatcct tccctttgcc
     1021 ctcaatggag accgccccgg catacaggat attcttgctc cggctcatga cgttccaccg
     1081 ttccgcctcc ttcacgttct tcatggtatc atccagcaga atcaccgggt aatgttccat
     1141 gccgccgtta aacccttcac ttcccggttt tgtgaaatac ttttcgccgg gaaacatgcc
     1201 ctcttccagt gtggccagga ggggagcgac cagctttttc accagccaag ttttgcccag
     1261 atccttttcc ccggccatga ccagatgctg aaccctggta cgagccgtgc gccttttcag
     1321 gcacgcctga atacgctgtc ttgccccctg catccaagct ataaaggaag gaaattggtc
     1381 aaggtcctcc gggtcatcca gcaaaccacg caagaaggcc agggctgtcg agcaatcccc
     1441 ttcctttccc tctacccacg gcccggacag ctcggaaatc gccgtctgaa ataacgggga
     1501 agcccgcttc aataatatcc acgcccagcg tttccagctg catggccacc tgtattttct
     1561 gctccacgtt catcgccgca ccggggcact gttccccgtc tctcagcgtc gtatcaaaaa
     1621 tatgtatttg ttctttcatt tacagtaaaa atgttaagag gtcgttcctt cgggaactgc
     1681 gcacggaaaa cgttcctgtt acggaatcat tcctttggtg caaatcaaga cagtcgttta
     1741 cttccctcgc gccggaacgg ctcaccggcg catatcaaga tatcttcacg gacttaccgt
     1801 ccgagaagca gcaatagacg accttgcaga aacagggtat tcatcttgct tgtttttact
     1861 ataaatccac cctgaatggg gaacgggaca gacaatgctc tttaaaacct gctctgtcaa
     1921 gacacctcaa atacatatga cattgttaga gttgtctcat ccattgaaat tttgaaagag
     1981 agtgccttcc ttcacaaaaa aactgcccag ctgaattaaa tcagctgggc agaacatctt
     2041 ttttatccgt ttctcccaat aagtttcaat ccacgcacct gtgaaggtac gatatcgtcc
     2101 atctgaaaac gctacagaga ccattatctg attcacccac ttataaaagt acggctaaat
     2161 tgtaacagat aatctgagcg cataaaaaga gaaactctct ccagacgggt gggaattatg
     2221 attagaattt tcgaaattgt caacctcgcc gataaaaaaa tttttcttat taggagacac
     2281 ggaaaggcat ttgccaaaaa tccgaattcc tgttaataaa gctccgcggg aacaaaaaag
     2341 agcaaggtac gcccatacct cactcttttc ttcctgaata taacctcatc tagaggctga
     2401 catcagagtt gctacacagc aaccgcctta taaaggttca ttatgatgat gtcaaatcaa
     2461 gaagcaaagg caatccatta caatgacgac taaaccctat atttatcggt atcttatacc
     2521 agcaatagca acaatttact taatcgtggc accaaacggg ggattctata tccaaaatat
     2581 tgtgcatgat tctgttaaac nnnnnnnnnn ctgttaaaca aggctatact gtacggataa
     2641 acatagaact ccaataactt gaactcattc ctgaaatcag ttccagatga gaaaattatg
     2701 tgaacccaaa ctgaataata cgcctaataa caatctatta agattaccat aggataaggt
     2761 ttgacacatt attcacaact caacggccta ttgctattca cagcaatagg ccgttttcta
     2821 catttcccgg tgggaacgta tttcctgaat ggaaatggac agggaggcgc ggccgcggta
     2881 cacgttgcgg tcaatcgtga aagcgatatc ccagggagga ttgggaagct cccgttccgc
     2941 tccgttgaag aaaatagcgt cacgctccac gatgccctgg cgcatgaaaa gcttcagatg
     3001 attggttccc acgcgcttgg gcggttccgt gggaaaaacg tcggaactca tgaagagagg
     3061 ctgtggattg gagttgccga aaggttccag cttttcatag ctgtccagca aatccagcgt
     3121 caacgcctgg aaggacactt ccatgtctat gttgagcacg gggctgcgct gttcctccgt
     3181 cgtggtttca gacacataac ggttgaatgc ccggcggaaa tcgtccatgc gggattcttc
     3241 aatcaccagc cccgccgcca tgtcatggcc gccgccggaa accagcgtat ccgcacaatg
     3301 gtgaatggcc tgcaccaggg acacgccggg aatggaacgg ccggagccct tccccacgcc
     3361 gctttcgtca aaggcgatga cgaaggtcgg cttgtggtac cgcctcatca gctgggaggc
     3421 tacaatgccc accacgccgg gatgccacgc gcgggaaccc agcacaatga cgttgtccct
     3481 ctccgggtca aaggagttat gaagcatttc caccgcatcc gtccggatgg cctcctcttc
     3541 ctcctgccgc tttcggttgt gggaatccag catttgggca agctgcacgg ccctcctggc
     3601 atccatggtc agcaggagtt ccaacgcatc catgggggaa tccatgcgcc cggcggcatt
     3661 aatgcgcggg cctatcctga accccacgtg ggcggcgttc aggaagcccg cgtggttgac
     3721 ggagtcggaa ggtcggatgc ccgctatttc cgtcagggtt ttgaggcccg tatggcggct
     3781 gtgcgccagc ctgcccaggc cgtggcgaac cagaatcctg ttttccgcca ccaggggaac
     3841 aatgtccgcc acggttgcca cggccaccag gtccagataa agtttcaggt caaaggtttt
     3901 cagtttcctc tccttcagga gggcatgcgc cagcttgaag actacgcccg cgctgcacag
     3961 gtaagtatat gggctgtttt cctcaatttt ggcattgacc accgccgctg catccggacg
     4021 ccccaaaggg cccgcctcat ggtggtccag aatgatgacg tctatcccca ggctgttaag
     4081 catgtccacc tccttcacgg aggaagtgcc gcagtccacc gtaataagca ggctgggagg
     4141 gtccgcacat tcgcaaaggc agcgcttgat gccggcctcg ctgagtccgt agccttcccg
     4201 ggaacggacg ggaataaaat actggggatc caggtcataa gccatcaaaa tggcccggag
     4261 aagcgccacg gaggtgacac cgtccacatc atagtcccca taaatacaca cggtttcccc
     4321 ttcatccacg gcccggaaaa tgcggtccac agcagccctc atttccccca tcaggaaagg
     4381 gtcgctcagg tgggaaagcc tggggtccag aaagagttcc gtttccattc ccccggtgaa
     4441 gccgcgctgc agcaggagct gcttgaccag caggggcagt tccgcaggaa acgctttcaa
     4501 gaccggatca tcctccttca cggaagggcg tagagtccaa tgaaaacccg gcgtcatggc
     4561 agtattttag ccccgcatcc aacagggtca aggttgtttc cgcaacagac ggccaaagcg
     4621 gagaaaagaa gcgtccgctt ccgtttccgg aaacagtccg cacgccgcgc gcccggccct
     4681 ttcaaaaaaa actccgcccg gttcgttccg gacggagttc ggcgtaaggc gccagcgcct
     4741 gagacggtta tttcctggag gaaaaaatca cttcattaat gattgcatcc ggctggggag
     4801 ccttgtaggt caggcgcacg gcggaagttc cggcaggaat ggcaaattcg gagaccccgg
     4861 gaccggcgtt tctcttgccg accggcgtcc aggcaccctc gccgcggcgc acctggatgg
     4921 cgaaggcggc ggatcccact acgactacct tggaagccct ggggttgtcc agattgggaa
     4981 cgactacgtc caacggctta tcggcacggt aaaccgtctt caggtttctg tcgctcaggg
     5041 agaccaggct ggtgccagga tcagaaggag gaacgtcaaa tttaaacatg ttcagaacaa
     5101 tgtcctgttc cttcccgctt ttattgacca gtttcatgcc tttgatcccc ttggggagct
     5161 gatttcccct ggcaatgaag ttcttgccct gtttgtccag cttctggacc acgggtttgg
     5221 aggaaccttc cacatccagc actacctcag cccaggagtt gacgtcatcc cgttccaggt
     5281 tgacttccag ccaggtggcg tccgtggggc catccaaccg cagggaaata aattctccgg
     5341 gtttcatctt gtgcgtttcc attacgcggt tgattcgcac gattttgtcg gatttctgca
     5401 cggaaacgcc ctccagccca gccacgttgg aggaagccat agcggaagga ggcgtgttga
     5461 cggctatttc cctcacggca gtccagacgg cccgtccgtt gcccgttttc ttcggctcaa
     5521 tgacgcggta gcgcacggca cggagcaaaa ccggcttggg agccttccag gctacctgca
     5581 ttccggcggt ctccggcccc agcggcttcc aggtcttgag gtcgcgggag ccttccagct
     5641 gccctttctc tacatagtcg ccgtccttgt cgttgcggcc catcagcact tccacgctcc
     5701 gcacgggaat gggcattccg tagtccacgc cataccagtc tcccggctga ccataagcgc
     5761 cggagtgcca gaaggagccg cggtccccgt cacagaagag ttccgcctta tccatgctgc
     5821 cccccttgac cagaggcttg ggggaaagag ccggagcgcc ggcaatggcc gggaaaactt
     5881 ttttggagac aatatccgcc agttcattga cggcgggggc catcacccgg gaaccggtct
     5941 ttaccgcgga acggtacagc tgcccttcct ggttatggcg gcgggaaatt ccatccatag
     6001 ccgccaaagc ctgcgtggcc tgcaccaggt gggtcgcggc ctctccggcg ttgttttcct
     6061 ccagcgccgc cacggcatgc tggccagcac gccccagttg ttcaaaggcg tccacccacg
     6121 cgccgatttc cttcatcagc cggggattgt cggccttggc gcggatgaca ggcgcagcgg
     6181 aagccatgcg agcgaattcc tttttaagca gagccgcatc agccttctct atcctgcctt
     6241 cccgggcggc ttccagcacg cgcttgacca caggttctat ttccacggat tcctcacgcc
     6301 ggtaaccgtg gccgttaggc ccctggtcgg agttgtggtt cacaaaaacc tgcatggctt
     6361 ccgccgcctt cgggaacagg cgcctgactc cctccttcca ggattcctcc gacttaaagc
     6421 cgttgatgtt ccaggagtag tccgcaagac cgaagaggga aaccttggag gcctccggct
     6481 tatccatggg gttggagaca aatccgccca tggattccct ggcccccggt tccgtggcaa
     6541 gtccgtacac gcggcccatg cacaggttgg aacggcagta gtccgtaacg gggaaattcc
     6601 accagacgta ggaagggcgc ttgattctcc tgttcaccca ctgctggcct tccagcgtga
     6661 tgtcatggca gacggaattc cccgtccaca taacgtggat ggaggggtcc aggcggtcgc
     6721 ccagaatatc cagataagtt cccggcttgg gattggccca gccgcggttg tactccgtag
     6781 ggcacatgac caggggagtg acgtctttct tcaccttgac aaattcgtca ttgattttgt
     6841 tcagcagaag ggcctgcatg tccccccgct tgccctctcc gaagatgtcg tcaaaaaaca
     6901 cggcaaagga acggacgccc agcttgtaca tcatctcaaa ctttttgatg acgttgttca
     6961 tgtcttcttc cgtccactta atgtctttgc cggggtggat ggcccagaca aagtccacat
     7021 ggttttcctt cgccaccttc accagttccc tgatctgggc ggcctggtct gcgggatagg
     7081 gatcccgcca gtggggggag gaatggtagg gatcgtcctt cggcccgtag atgtatgtgt
     7141 tcattttgtt ctgcccgtaa aagcgcagct ggctcagacg ggcttcatgg ctccagggag
     7201 tgccgtaaaa accttccacc gttccgcgga attcaatgtc cggccaatcc gtaatttccc
     7261 caacgggcaa aatcacgcct tctccgccgg tcttcgttcc cagctgccgc agcgtctgca
     7321 tggcgtaaaa tgccccccgt tcgtcatggg ctcctatgcc cacctttccc tgcggagaaa
     7381 tcaccagctt gtaggctccg gatttttccg gaaccccgtc cagcagcttg ctcccgcccg
     7441 ccttggcgga acgtattgtc acggaaggct tcccggagaa agcaaccgtc tgggaggtga
     7501 atttggactg ctggggggac ggatatacgg caggagtcgc cgccagagcg gaaccggcca
     7561 gaaatgccgc cacgctcaag ccacattgga gaagttgaaa accattcatg atgatgcgcg
     7621 ataatggaac cagaaaaaac tttccacctc aagaaaaaac cgttctcctc catcaggctg
     7681 tcgccaccag gggaaaaaca cgggaaaaga cttcaaaaag aaacttcccc gccttctctt
     7741 ccctcttcag gaagatctcc ccgctcctca tggaatattc gggaactgtc atcactccat
     7801 cacctggctc atccagagac cgcccggcgc agccgcatcc ctgcaggaag cggcatgttg
     7861 atggaatttc ctgtcaaggg caatcgtccc gcacggaaat cctggcgggg ctggatgcca
     7921 ggggagccgc cttctccctt cttgaccaaa aattcaccgt aacgctgaaa aacaaaaaag
     7981 ggccgtgcag aatccccgaa aagtcagcgt catctcattt cagaagacaa atttgaccat
     8041 tgcatcactt gaagggaagg ttcatcatgc ggaacatgct ccggcgcatt ttgattcctt
     8101 ttctgtgtct tctgggcctg ttctgctctc ttcaggccca ggatgcccca gtattgaaaa
     8161 tcgcggctct gcaccccgtc ttgggagaca tggcccgcgc catcggcggc agccgcgtgc
     8221 aggtggcgga cctgctgcgc cccaacggca acctgcacag ctttgaaccc tcgccgcagg
     8281 acattgccgc cgcaggacgg gcgcgcctgg tgctggcctc cggcaaaaac ctggagccgt
     8341 accttcccaa attaaaggac gccctgggag gcagcgcgca aattctggat ctcggagcct
     8401 ccgtcccgga tgttcccgtg gaggaagaca ccgcagacca tgaccacgaa cacgacggag
     8461 actgctgcgc ccacggtccc aacgatcccc actggtggca caccccggcc aatatgaaac
     8521 gcgccgcccg caccctggcc gcaacgctca cgcggctgga cccggcccat gaacaggact
     8581 ataaagccgg cctgtcccgg tggaacagaa agatggacca gctttcctcc tgggccagaa
     8641 aggaacttgc ggacattccg gaaacggacc gcgtcctggt tacggggcat gcggccatga
     8701 accatttctg cagggaattc ggcttccgca gcatcagcat ccaggggata agccgtgaag
     8761 atgaaggcaa ctccgcccag ctggcctcca cgctgaaaaa gctgaggtca gccggggtaa
     8821 aagccctgtt cccggaatat tcctccaacc ccaagagcct tacggaaatc gccaaatccc
     8881 tgaacatccc cgttgccaga ccaatcaata ccgacgggct ggcccctgag ggccacacgt
     8941 tcgaatccat gttcaaacaa aacacaggca tcatcaagga agccctttcc ccgtccccca
     9001 ggccatgacc agcacacccc tgcggcagtt ttttcccact ctgggagtcg tcatcctgct
     9061 gggattcttc ctctacaaca tgacggcacc caccaccgga tcacaggaag cggagcattc
     9121 cgcccccacg gcgcaaaccg cggatgttcc cacggtcatc acggtcagat gcgcccaccg
     9181 tccggaagcc ctttccatct cctgcggcgg ccgtcttctc tggcagcccg aaacgcccgg
     9241 gctgcatgaa gaaacggaat gcggcctgcc gttgaacaac ggttccgtaa cgcttaccgt
     9301 ttccgcccgc tggccggaaa acacgccgga cacccccgtc acgctggaac tggaaccgga
     9361 ggggaaaacc accgtctccg ccacccgctg gtccttcggc ccctccctga acgacagcta
     9421 ctccttctca tggaaatgaa cagcccagcc cctccctgca cccaggacca catctgctgg
     9481 ggcgcgcacg ccagccaccc ggaccgccac cgactggaag tggacagcct cagcgtttat
     9541 tatggaagcc ttctcgcgct caacggcatc agcttttcca tcacctgcgg ccacacgctg
     9601 gctctgatgg gtcccaacgg tgcaggaaaa tccacgctga tcaaggctct ggccgggctg
     9661 atccgccccg attccgggaa aatcctgtgg aacggatgcc ccctgcacga cacgccggga
     9721 gaaatagcct acctgcccca gcgttccgac gtagactggt ctttccccat caccgtccgc
     9781 gcgctggtgg aaatgggccg ctatccgtcc ctgggcctgt ggaaaaaatt cgggaggcat
     9841 gaccgggata ttgtggaaaa atcccttcac gttcttggta tggaatccct ggcggaccgc
     9901 cagatttcag aactctccgg cggccagcag cagcgggcct ttctggctcg ggccctggcg
     9961 caggaggccc atgtcctcct gctggacgaa cccttcacag gcctggacgc ccccgccagc
    10021 caatccctgg ggaggcttct ggattccctg gcggcggaag ggcggctggt catcgcctcc
    10081 caccacgacc tgaacacggc ggcagacatc ttcgacacca tcctgctgat gaacagggag
    10141 ctggccgcct tcggcccccc gaaggaagtg cttacccccg cacgcatccg ggagacatac
    10201 ggaatggaac ctcaaccgga aatccaagct tcatgatgga tgattttctc caattcctcc
    10261 gcgaacccat cgctcagcgg tccctgctgg cctgcgtcat gattggcttc gccaacggtt
    10321 tcgtcagcgg gctggttatc ctgaaaaaat ccgcccttca aatcggcacg ctctcctgtg
    10381 ccctgttgcc gggcattgcg gtggcggtgc tcctcttcgg ccttacccgt tggagtttgc
    10441 tgacgggggc ggtggtggcc gccctgctgg tgggcctggg gtccctcttc gtttcccgca
    10501 catcccggct gaaccaggat acagcc
//
LOCUS       DAWEMX010000089        10409 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2702_length_10409_cov_14.179641, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000089 DAWEMX010000000
VERSION     DAWEMX010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_10409_cov_14.179641"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2183 a         2792 c         3155 g         2279 t
ORIGIN      
        1 cgggaagggc cacgtagccg cagggctggt aggggggctt gccggaagcg accctctgga
       61 tagtccattc cgtggctggt ttcccattga cgattagctg ggagtcctga atggagaggg
      121 tatctcccgg aatgccgcag aggcgtttga cgtagtgggt tcctccttcc tggccggtgc
      181 tgcttccctt attggcgatg ccttcgatgc cgcgtgtatc aaagacgaag acttcccctc
      241 gttccggctt gcggaagtgg taggccattt tattcaccac gattaggtcc ccggcgtcaa
      301 aacgggcatt gacgattgtg tctccgggca ggtagcttct gaacggggtg tgccgttcgg
      361 cttcaaagcg cggttcctga ttgaggaagt agcggcgcgt ttccgcttcc gctgccggaa
      421 tttccagctt ggagccgtca tcgaaaatca cgttggtcac cgtgagcaga agatacttgg
      481 tggcaggttc gaaacggacg attttctttt ccttggaggc cgtttcgtgc acataggagc
      541 cgccgtacag gatcatgtcc ccgattttct gcatcagggt gggatcccct tcctggggaa
      601 gggcgcggat tccgttcagg gagggctgca tggaaccggt cgggatgcgg aagggctgga
      661 ctacataaca gcgcaaaccc aggaaaatgg ccagaatcac gaacagaact tccacgtttt
      721 ccgccaaggc ggaaggaacc gctccgggga tggattccag cgtggattcc agtttggcgg
      781 taatggtttc cgcctgctgc ctgtcacccc ggtaaagggc cgttttcagc aggtcgcggc
      841 tttcttcaaa ctccgccagg cgttcgggag gcagaagatc gcgattgtag ttgatgtagc
      901 ggcgcactgc tttcagggcc atacgtcctt tccggcgcca tttgggcgtg aaccaggcca
      961 gaggatgatc cagaagaaac accagaacgg cgtttgcagc cggaaagcaa tcgtaaaggg
     1021 cagctaggat ggacttgtgc gggctattca tgaagtacgg cattcatgat gccccgtccg
     1081 gaggcgtcat ggaagtttat tttacgttac ggaagcccca tttttccagg attcagcaaa
     1141 ttttttgggt ccagggcgga tttgaccagt tcgtgcaggt ggtaggcgcc ttcccccacg
     1201 gctttccgga accatttggc cttcgccagg ccgatgccgt gttccccggt aatggcgcct
     1261 ccgtgtgcca gcacccagtg gaacagcctg tccacacagg cgtccgccag ggctttcttc
     1321 tctggattcg cataatcgtc caccatgatg ttggtatgga tgttgccgtc cccggaatgg
     1381 ccgaaacacg cgatgtccag ccctgtttcc cggttcaggg cctcgcagaa ttccaccagg
     1441 gccaccaggc gcgacctggg gatgacgatg tcttcgttga gcttggtcat tccggttgct
     1501 ttcaggctgt aggaaaattc ccggcggagt tgccagatgg cttccgcctc cgcttccgtg
     1561 tctgcgcgca gaattccggt cgcgccgcat tcttccagca gggtgcatag ggaatccagt
     1621 tcctcacgca cggcgggccg cctgccgtcc acttccacaa tcaggtggcc gcggcagccg
     1681 gaaggcaggg cgctgtcccc cagatagttc cgggcggcgc ggagagtgaa actgtccgtt
     1741 atttccaggg cggagggaag atgcccgctg ttcaggatgc gttgcacggc ggcagcggcc
     1801 agcgggaatt gcgggaagcc gacgctgagc gcgccgaatg cttccgggtg ggggatgagc
     1861 ctcaaaacgg cccgggtgat gatgcccagc attccttccg aaccgataaa aaggtccccg
     1921 atatcgaacc ccgtcttgtt tttatgggtg cgtccgccgc attccactac ctctccgctg
     1981 gcaagcacca cggtcagccc cagcacgtaa gctctggtga ctccgtattt caggcatctg
     2041 gggccgcctg cgttcgtggc gatgtttcct ccgatgctgc attcctttct ggaggccggg
     2101 tccggcgggt agaaccatcc cagagcacgg gcttcccgct ggagatccgc tgtgataact
     2161 ccgggttcca ccacggcaac gccgtcttcc ggggatatct ccaggatacg gttcatccgt
     2221 tccagggaaa ggctgatgcc tccgcgcacg ggaacgcacc ctcctacgta tcccaccccg
     2281 ccgccgcggg ccgtgacggg gatgttcatg gaggatgcgt agcgcagcaa cagggagacg
     2341 tcctccgtac tttccgggaa aacgactgcc tccggcagca cggaggcgta ccatttgtcc
     2401 ccggcgtgcg ccgccagcac atccgggact ctggaaactt tttcaggccc cagaatgcgg
     2461 gaaatgtctt ctttcaaaga catggattat ttgagaatgg gggtgcagaa gcagtccggg
     2521 cggtggaaat ccggattgcc ggaaaggtcg tccgccgtag tcaggaaaat ctggtccgga
     2581 gtgtccagga tgaacgtggc tgccagcttg cagtcgcgga tgtccacgcc gcgcagttcg
     2641 tttagcggaa tccgggccat ggcgcaccat ccctcgtccg tcagaatgca cttggtatcc
     2701 acggccagcg gagcggggat gtcttcattg gcgcggcggg tatcggaaaa ggcgcaggcc
     2761 caccatgcgc cgttgggcgc aaggttgaat tcccagtaat tggtgccgtc tcccgcggcc
     2821 atgaaccatt ccgccaggtc gtaccgccat agctccggct ggaactggcc gggccgggct
     2881 tccggatgga tggtggctgc cttgctgcgg gaagccagaa accagaggaa ttctccgtcc
     2941 gtggcgaagg cgaattccgc acttggctcc acatcatagc cgaaccagtc gttgacgagt
     3001 tcctggcggg gcagggaggc aagctggtcg aagcttccgt tgaaggccca ttggtgaatg
     3061 attacttgca taccaccaca tttggacagt tttcaggttt tatcaagcca gaacgtctta
     3121 caggcttgga tattgacgag atttctccat catgggggcc gtcatgcatt ttcaaaggac
     3181 gctgaatcgg ttttcccgcg aaaacgttcc cgggtatctg gctgtgttca tcggcggttt
     3241 cggggacatg tttgcgggtt gtttgagccg tgtggagaaa ggatttcccg gttttttgcc
     3301 gggtgtggcg tacgcaacgg cttattatca ctgggacgga ggcggctgcg gcgtgttttt
     3361 tgacaggtgc ggaaaaatag cggtggagct ggaagagctg agacggagat atcccgggct
     3421 gcctgtcgtg ttgataggac atagctacgg aggctcctgc gcagtggaag tagccaggcg
     3481 ccagtcggtt caggctgctc ctctttgcct cctgacggta gatgccgtgg cgcgcaggca
     3541 aaagagcgtg cgtccggcct gtgtggaatg gtgggggaac gcttacctgc gggacggcgg
     3601 cggcttcatg gacgcggtgc ccagaatagg cggacgctgg ggacattgcg ccggggctga
     3661 tgccaatctt gctttttccg gctacagtcg tgatcgggag ggccgccttt attctcaccg
     3721 gcgtcctgcg cccatgcttt atgagccgcc tggggaagaa gcatgcagcc tttttcaagc
     3781 cgctgcctcc tggctggaaa gaaatcttcc ggactgaact gccggagccg tgctaccggt
     3841 tcagttcatc ccacttcacc agttgggaaa gaagttggct gataaggcgt tctgtctgtt
     3901 ccctggcttt ctcttccggc actctgatct tgtccccgta cgtggtggaa atctggaaat
     3961 agcaccagcg ttggtccatg ccgtgcagaa ccctgtcttt catatccatc agggtgcgga
     4021 tcaagtggtc ttccgtcatg tggcggtggc cgataaatac gtaataatgc atgttgtcca
     4081 gaatgactgg ttgcggatgg ttcggagcga tgttctgctg ggatttcagc ttggtcatcc
     4141 tgatggttcc gcggccttcc acgggaacat ccaccgtggt ccccgtcagg ttaaaatggc
     4201 cctgggccgg gaggcagcgt tccgggcggt ggatggagtt gtttaaatca tgaccggatt
     4261 taacgatgga aacgcggcac agaacgggta cggtttccgt cgcatccagg ctgatgggga
     4321 gttcctgcac gtaatccgct ttggaaaatt ccgtatccgg ggccaggatg ccccgttcct
     4381 gttcggattc ctgccggcgc gtgccgtgcc agccgtaggg attcatgcct gtggggagat
     4441 ccatgctgat ggatgagtcc tgtacttccc ccctgcgcgg catcaggtaa gtgccgccga
     4501 gcatgacggc cagcagaaaa gggatgatga gaattttgag cgttttaata ttcatggcgg
     4561 cggaaacgct taatgggact tgttcatttt aacaaccagc ttcttgcgct gtgacggctt
     4621 ccagatgagc tccccggcca gcagggagtg gacggctgcc aggccgaaaa ggctgatggg
     4681 gaaaaagaac agcaggccgg accagtcatg ccaggttttt gacgcaaact cgggatttcc
     4741 gtattccgcc attaccacga tgctggtgat gcggacaccg ttgccgatga cggccagggg
     4801 aatcgcgctg agaaagagca cgcatttttt ccagaatttc aagtcggaca ggtaggccca
     4861 ggctgcggac agcatgagca gtgccatcag ggaacgcatg ccgctgcacc ctcctgcaat
     4921 attgaaggca tcccagtgtc cgccggtaga gcggatattg gtgccctgca gataggtatc
     4981 caccccgaag atgcttgccc cccaatgccc cagttccgtc gcgatgattt gaagtcccac
     5041 cgtagcctgc tggaaggagg gcagggggat gcacagccag aaaaagagca gcgggaaggc
     5101 gcacagcctg gcggtctgcg gtccggccag gtaccaggcc ccgcccagta aaatcagggg
     5161 aagagcgcct acggccacgc gaggctgccc cacgcggaag gaaagcatca gaagcatgat
     5221 cgccgggatg aaaagaatca ggccgcgcca gtccatgcgt ctgggtgcct gggcaatttt
     5281 ctttctggca tggtacagca tgaagacgat gatgggaacc accagccagc cgtgttcata
     5341 atccgtttgc ttgttccagg aggaaatgag ccactggatg ctgttttcgt ttccgaatga
     5401 accgaattcc gggaatgcca gatagttcag cgccagcagc agcgtgctca cggccagggc
     5461 ggcgatcatg ggggcggtca gaaatctctc tttgccatca ggagcggggg aatccatcat
     5521 ggagaaaaat caaattgtga gaattaagta ttgcgattat gcgggcctgt gttcagaatg
     5581 cgtccgcttt tcagtttccg gtatgaagat tatcagcggt acagcacaca gagaacttgc
     5641 ggagcgcatt gcgcaaagcg taggcattca gttgacgaac gtcacggtga atactttccc
     5701 cgatggggaa agttttgtaa agataaatga aaacatccgg ggcaaggatg ttttcctcat
     5761 tcagcccacc tgccctccca cgaaccataa tatcatggaa ttgtgcgtga tggtggacgc
     5821 cgcccgccgc gccagtgccg gacgcatcac ggctgttgtt cctttcttcg ggtatgcccg
     5881 ccaggaccgc aaggaccagc cccgcgttcc cattaccgcc aagctggtgg ccaatctgct
     5941 gacggccgcc ggagtggacc gcgtgctgac tatggatctc catgccgccc agatccaggg
     6001 attctttgat attccggtgg atcacctgta cgccgccccc gttttaatcc gtcacctgcg
     6061 cgaacattat gttaaagacc tcaagaaact ggtcgtcgtc tccccggacg tgggcggcgt
     6121 gaaaatggct cgcgcgtatt ccgatgcgct gggtgcggaa ttggccattg ttgccaagca
     6181 ccgcgtcaac gccacccatg tggaagccat gaatgtcatt ggtgacgtgg aagggcgcga
     6241 tgccgttctg attgacgaca tgacggaaac cgccggcacc ctgtgtgctg ccgccaatat
     6301 cctgaaggaa cggggagccc agcgcgtgtt cgcctgcgtt tctcacggcg tgctggggga
     6361 catggcccgc gaacggattt ccggttcctg cattgaacgc gttcttacct ccgacaccgt
     6421 tcccatggcc tatggcccca aagtggactg tgtcagcgtg ggagacctgc tgggccatgc
     6481 cgtgcagcgc atacatgacg gagaatccgt atcatcatta tttgacattt agacaatttt
     6541 agtgcagagt cctgcgccct gacctgagta ccggtttacg cgggttcgga aatcccggcg
     6601 gggcctctgc ctgaacccga ttaggaaaca tcatcaatca acactcaata ttgcctctat
     6661 aaaacatggc tacatcccat tccctcaagg ccgaaacgag agcctgcggc tccggtaatc
     6721 tgaagcaact ccgcagccag ggcctggttc ccggcgtggt ttacggtccc ggttttgaca
     6781 acgtcaatat ccaggttgac gcccgtgaat ttgcccgcat gctggcttcc gccgtttccg
     6841 aacacatcct cgtggccctg gatatcaacg gcaagatcgt gaaggttctc cttaaggaag
     6901 tgcagcacaa ccccatcacc aacgcctgcc tgcatgtgga ctttcaggct gtgaccgata
     6961 ccacggtcat ccattccatt gttcccgtca ttctggaagg cgattctgcc ggcgtgtcct
     7021 tgggcggcgt tctggaccag accattcacg aactggcgat tatctgccag gtgaaggatc
     7081 tgccggaagc cattaccgct gacatctccg gtttgaaact gggtgaaagc ctgcgcattg
     7141 ccgatctcaa actcccctcc ggcgtaacca cggaactggc cggcgatgtg attgtggcta
     7201 ttgtggaagc tccccgtgtt tccggcgagg aagcggctcc ggctaccgaa gaagctgttg
     7261 ccgagaaata agtttgatac gcctgtttct tcttttaccg ggtccggagc cgtttttgcg
     7321 gctccgggct tttttttgtc gccaacaggg catcttttgg taaagtgcag gatacgtatt
     7381 tgcctgatca atctggaaaa agccttagat ttatgaacaa gtccctcata ttggtgttga
     7441 ccgcattcgt tccgcttgca gctgcagatg aagtaaaact caaggatgga accgtttaca
     7501 aaaattgcac ggtagaagtg gaaacgccgg aaagcgtcag cctcctggtt cccgtgtccg
     7561 gaggtatcaa ggattccgta accgtgaaga gggatttgat tgaatccatc aggaaggcga
     7621 ctccggatga actggaggct gcccgaatca gcaaaatgta tgccaaaccg gaaaccatga
     7681 atgccgggga tttggaaaaa gccttggcgg acctggacaa gaccattaag aaaaatcccc
     7741 agggccttgc tcatgacgcc gccgtcaagg caagagccag agtagtggtt ttgctggagg
     7801 aaaggaaatt gacggaggaa gcccgggccg cccagaatgc cagggaggag gcggaagtga
     7861 ccgtgcgcac caaatacgac catgaggcca ataagctgct gaagcgtttc aaggcgcttg
     7921 ccgtgcgcaa tccataccag gccatggccg tgtatgaccg cttgcgcgac ggttatcccg
     7981 gttccgccgc cttggcggat gcctatccgg atgccgccag aatcgccgga cagctcaacc
     8041 gtaagctgga ggtcatgatc gccgccaagg aaaagtcctt ggagaaggaa cgtgaagccc
     8101 tgagaaagga ggaagagaag cgccggggaa atcccaaact gaccaaggaa cagcgccagg
     8161 tgttgatgga tgctttccag aaaaggcaga ctgccatccg ggaacgtgaa aaccagctca
     8221 cggaagtgta ccgggctttg cgcaagaagg tcaaggaacg cggggaccga tggtttgaac
     8281 ctacggcagg ttctttggaa gccatgagag accttaagct ggtagccgct acggatgcgg
     8341 aacgcttaaa aaaacaggac ccggaaaacg gagccggttc cgctgcgctg gaaaaggcct
     8401 ggaatctgtg tgacgagaaa aaatttgacg aagctatgga ggttctgacg gagctccgga
     8461 ccgcccaggt tcccagggaa tactatgagg aactgacgga aacggtgcgc gtggggttgc
     8521 gggaacagcg cgcccgtgaa cgcgccgaac gtgcggaagc ggcgcgcaaa gcccgtgaag
     8581 accgggataa gaagcgccag gaggaaagag aggcggccgc caaggccaaa aagaaataat
     8641 tctgccgttt tccggagaaa actctccgga gagatatggc ggaaatggcg tttacggacg
     8701 ggatggatca agtatgtgtt gattccatcc tgcccgtcag gctttccggc cgttgatttc
     8761 gcgcaattcg ttgtcagaga ttcctgaggt ttttctgtcg gagcggggcc ggaggcgtgt
     8821 tcctttgact gtctgcatgt ttttgcgggg agaagagggc aaatctttcc ccatgcgata
     8881 tttcctgctt tcttccgccg cgtcttcagt atagaagtgt tgcatgagga acgcttcttg
     8941 caaacgggtc acgggtgaaa cggacatcag catggaactg aacctggacg gcacgggatg
     9001 cgccgccgtc gcaacggggc atgcgttttt tgaccatatg ctggatttac tggcccgcca
     9061 ttccctgatg gacctgaccc ttcaggcccg cggagacctg gaagtagacg cccaccacac
     9121 ggtagaggat gtgggaatcg ttctggggga atgcattaaa aatgcactgg gggacaaaaa
     9181 gggcattgtt cgctacggct gttcctacct gcccatggat gaaactctta cgcgcgtggt
     9241 gatggatttg agcaaccgtc cgtatgtagc tttccgtatt ccggaaggcg gtttgccgga
     9301 tgcccccaac tttccgctga ccctttgcga ggaattctgc cgtgcgctgg ccaataacct
     9361 gcgttgcaac ctgcatgtgg aagtgcttta cggcagggat ggtcaccaca ttgcggaatc
     9421 tgtcttcaag ggcatcgccc acgcgttgcg gcaggctgcg gcgattgatc cccgagccgc
     9481 cgggacgctg ccttcaacca agggaatgct gtaaaataca tgaaacttgg cgtgattgat
     9541 tacggtgcgg gcaatctccg cagtgtgctg aacacctttg aggccgccgg cgtgaccggt
     9601 catctggtgc gtactccgga agatgcggcg ggcgtaaccc atttggttct tcccggcgtg
     9661 ggcgccttcg gcgactgtgc agaaaagctg cgccgccaga atctggtgcc cctggtccgg
     9721 gcatggatag agcgggacag gccttttctg ggcatctgcg tgggctatca ggttcttttt
     9781 gaaagcgggg aagaaagcgg gcaggttccc ggactgggta tcctcaaggg gcgcgtggtt
     9841 cgattttcag aatcggaact caaggtgcct cacatggggt ggaacagcgt ggctctcact
     9901 gatcccgccg atcccatctg gaaaggcatg gggaaggaac cgtactttta tttcgtgcat
     9961 tcctattatc cccagccgga ggatgaatcc ctgattgccg ctttctgcac ctacggcgtg
    10021 cgttttgctg ccgctgtcag gcgcggcaat ctggtcgcca cccagttcca cccggaaaaa
    10081 agccagaaac tgggcgtgaa actgctgaaa aactttattt ccctgtgagc cggaaattgc
    10141 cggctcacag actgccgctc ttcttcctga cagcaagggg caaggaggag cggcgaatca
    10201 aatgaaacgt cattctcgaa aagcagcctt attaaaaaga cgccgggatt aatttcattg
    10261 aaacccttcg aacggcacct ccgtagacct tcatggctca gtttttccct gtatggaaaa
    10321 tagccagggc aagaggagtg gcaaatttat ttcttactgc cggttctggt caatattgct
    10381 taggggggcg gggaatattt gttgagcgg
//
LOCUS       DAWEMX010000090        10343 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2722_length_10343_cov_13.730463, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000090 DAWEMX010000000
VERSION     DAWEMX010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_10343_cov_13.730463"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9242..9251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2307 a         3234 c         2802 g         1990 t
ORIGIN      
        1 ctatcctgga agagtcaaac agcaagcatt ccccggtttc caagtaaaaa agaaatgcct
       61 tttacacggt ccatagcagg gctattcact tgccaagtcc ggatgaagct gtaaaaatcc
      121 gcgccatgag aaaactcctg atccgctcct gtctgattgc cgggctggct tggacttctt
      181 cctgcgcctc caacaaggaa acggcacctc aggaccccgc cccagttcct gccgttcagc
      241 cggaacaaac cgcttcagct aaccagcccg caccctgggc gcccgctccg gcgcaacagg
      301 agattcttcc agaggatgag aatgaagaat ccgcaccggg gccggctgtc ttgccgggcg
      361 atttccgccc cggactgcgc accccccggc tgccggaaac cctgctgtac gatctggacg
      421 gcaagttgat taccccttcc gccccataat cttttcccac caccatgact gcgaccattc
      481 ccaagggttt ccaccccgaa cctttttcct accaccagga gcttgaactg gatattgacg
      541 ccctttccaa tgcaggcgac ggcatcggcc gcgtggacgg atgggtggtt tttgtcccct
      601 tcgccctgcc gggagaccgt gtaaaggcgc gcgtctggcg taatgacagg aattattcct
      661 ccgccgatct ggtggaagta attaacccca gcccggaccg cgtggaaccg ggctgccgcc
      721 tgttcggaac ctgcggggga tgccagtacc agcatttttc ctatgaccgc cagcttctct
      781 ggaagacccg gcaggtagcg gatctgctcc gcttgcaggc agggctggaa ctgcccgtca
      841 atccggccgt agcctccccc cgccaatacc attaccgctc caagattacg ccccattttg
      901 acaagccgaa ggaaggaggc aagccggcca tcggtttcct gaaagcaggc tccagaaggg
      961 acgtagtgga cgtgccgcaa tgcccgattg ccatggagtg catcaatgaa gccctgcccc
     1021 tggcccgcaa aagcgtttac caggccgcag cccggttcaa gcggggagcc accattctgc
     1081 tccgggcctc ggaaggaacc gtcatcacca ataacaatgc cgtcgcctgc gaacgggtgg
     1141 gcaatttgga attccatttt ctggcgggag actttttcca gaataatcct ttcattcttc
     1201 cgctttttac ggattacgtg gcccaacagg cgagtatgga cggggaggaa ttcctggtgg
     1261 acgcctactg cggttccggc ctgttcgcct tgagcctggc gaagaaattt aaaaaagtgc
     1321 tgggcgtgga agtcagcgag acttccgcag actgggcacg cagcaatgcc cgcagcaacg
     1381 gaattgaaca tgcggaattc ctggcggcgg acgccggagc catttttgcc cgggtggatt
     1441 tccccgcgga aaaaaccgcc gtggtgattg accctccccg gaaaggatgc agtgcggaat
     1501 ttttggccca gttatttgcc ttcggccccg gaaaagtcgt ttacgtttcc tgcaatcccg
     1561 ccacccagat acgggatctg gcggaattcg acaaggccgg gtattccgtc accgccgtcc
     1621 agccctttga cctgttcccc cagaccaagc atctggaatg cgtggtcacc ctgaaaaaaa
     1681 atacctgatt ttttccatgc ccaagcttta tatcaaaacc tacggctgcc agatgaacga
     1741 acgggactcc gaacaggtgg cccgcatgtt cgtgcagaag ggctacacca tgacggaccg
     1801 cgaggatgag gcggacgtca tcctgttcaa ctcctgttcc atccgggaac aggcggaaca
     1861 aaaggctctg ggaaaaatgg ggcttctggc caagcagcag cggcatcgtc cgcatgtagt
     1921 gtacggcatg atgggctgca tggcccaaag taaaaaagag gaactgttca aggaactgcc
     1981 gcgcctggat ctcgtggtcg gcacccagaa ataccaccgc gtatttgaac atgtggacgg
     2041 cattctgcgc gcgcgccagg aacgccgcat ggatgagttg caaaccgcct tttccggtac
     2101 gcatgtgtgc gatgtggcgg aagaggcgga ttcccagaac cgaatccggg atcatctgaa
     2161 tcccggcgtg cgctccacgg cgtatgtctc catcatgcag gggtgcgaaa tgaagtgcgc
     2221 ctactgcatt gttccctaca cccgcggcgc agaacgcagc cgccccatcc gggacgtggt
     2281 ggatgaggtg aagatgctgg cggacgccgg cgtgaaggaa gtcacgcttc tgggccagat
     2341 cgtcaaccgg tacggcaggc agatggaaac ggcaggtggc aagggcggct tcgtccagct
     2401 gctggaagcc gtgcatgaag tggaaggaat ccggcgcatc cgcttcgttt ccccgcatcc
     2461 catcggtttc cgccaggatc tggtgcaggc gttcacctac cttcccaagt tgtgcagcca
     2521 cattcatttc ccgatgcaga gcggcagcga ccgcatcctg aaaatgatgc gcaggccgta
     2581 caggaatgaa acctatctgg acctttgctc ccggatgaag caggcacgcc cggatttgtc
     2641 catcaccacg gatatcattg tgggcttccc aggagaaacg gaggaggatt acctgctgac
     2701 caggcaggcc gtggaacagg tccagtttga caacgcattc attttccgct attccccgcg
     2761 ccgcggcaca cccgccgccg tgatggaaaa tcagattccg gaagaagtga aggaagcgcg
     2821 caaccaggat cttctggccg tagtcaatga aatagctatc cggaaaaacc gggatctggt
     2881 aggcaccgtg caggaagttc ttctggaagg gccgtccaaa acgaatgcgg cgcgcctctc
     2941 cggccggacc tcccagaaca agcccgtcat ggtggatgcc gcccctgacc tggcgggaga
     3001 gatccttccc atccgcattg aggaaagcac gggatttact ctgtacggcg ttccatgccc
     3061 ctccagggga tgatttgcac gctcttttct tccatccgca gatagagggt tccggtttcc
     3121 cggagacggt atacggctgt tccctccggt acggggcatt catgctccgt cgtgaaaatg
     3181 accgcgcggg cgcctgtggc cctgatccac gccgcgctgt ccaccgggtc gcgcggatga
     3241 tgcccgatga gcaatacgtc cgcccgcggt gttttctccg cgcgcgccag ggaagaaaac
     3301 ccggtattcc ctatcatcag gaaccgccgc ccgcggcatt cccatgacaa cacccggttc
     3361 cggtcctgac ggattcccgt gcgcaacggg acaggaaaat cttccagcac aagtgcattg
     3421 cccggccgta acctccattg ccggacggga cattccccgg actccgcgcc ccagttccgg
     3481 attccgggat attccttctc cagttctttc tctccggccc gttccgcttt gccgtttccc
     3541 caggacagca tgccgtaggg ccggacgctg cgcgccttca gcacgggaag gagggtgtac
     3601 cgcaccgcat cttccgttcc gcttcccaca accaatgcgg gattggaaat gactgtagcc
     3661 caggcatcct tctgcaaggg aacgataaca gcttcatttt ccccggaagg cggtgcggaa
     3721 gaaagataac tgtacggcag ggaagctact cccccggcta cggacagcat ggcggacgcc
     3781 agccaggaag cgaccgtatt acagacatgc agcatccacg ggcaccacgc gaacatcagc
     3841 ccggacaggg aaatgcccat aaggggaaag acaagtacgc tcaggcacaa attggtcaag
     3901 ggagcataca ggttccaggt tccgaagtgc catgccgtca agggaatgga agccagccac
     3961 gcccccacgg agacgatcag cgcgccgcgg catgcttttt ccagccgcac caggaatctt
     4021 tcccgcgcat gataaatccg gggaggaata aacgggtccg gcgaccacag gggcttttcc
     4081 cggctgagcc attccgccag gcagacaatg acggcaaaaa cgcaaaaaga aagctggaac
     4141 cccggctgga aaacctggag cgggttcatt aaacagagga ggatgaaagc gagcgccaga
     4201 atattggccg gatgtccctt tctgcgcagc acaaacgcca gaagccagac tgtcgccatg
     4261 atgaacgccc gcagggcgga agcggacatc ccggtcacga acacgtacgc cgccagcaaa
     4321 aggatgcagg ccagccgggc cgcacggggg tgcacatgca aaagcctcag catacccaga
     4381 accagcatgg cggccacgcc cacatgcatg ccgctgacag cgaatacatg catgcagccg
     4441 ctttccagaa acttcgccat cgtttccggc ctcgcgtccg tcttgtcccc aagcacggcg
     4501 gaaaccatca cctgccgggc ctcatcatcc ggaggggcgc cctccctgag cagggaggca
     4561 gccctttccc ggagccgcag ggaaaaagcc cggaaacggc tgcgccagtc tcccggcccg
     4621 ttccggcaat actcatccag ccggattccg gcgatgatgc cgtgcaggta tccccaccgc
     4681 tcccggtcaa agatgccggg accgcacggc gcctgaagca gatacgcctc tcccctgatc
     4741 cggtagcgtt cccctgcctc cagagggcag gccccgtcac gggaggaaac cgcatacagc
     4801 caccgggaac catccgggcg aaacaacacg gattttccgg aaacagcctc aacggagcct
     4861 tccataacca tgactctccc cgcttccacg ggaacgcctg cgtattcccg ttcatacgcg
     4921 aaaagatata cggcggccca catcgccaga gcaacggcaa gcacccccat ggagggcgca
     4981 cgtaaaaaac acggcagcga cgccaggcag aagcatggaa tccagaacca aagggaaaca
     5041 tccattgccg cgcaggcgca cagcagggac aaagccggaa tcagcaaggg agcagatgcc
     5101 gtcatccgtt ccggccacgg acgcagaaca cgccgaactt cttccatgca gggtgaacct
     5161 tacggcatgg catccgcacc atccggctcc agcgggagga cggaaggcag cggaagaaca
     5221 acgttggcag gcaggggggc cttgcgctgg agcactctgg gagccagggc ctcgcttgtc
     5281 tcataagctc cgcggaacat ttccagcttg gcgtccagat tgtcgatgta atgcagggcc
     5341 accgcttccg gcgttttggg aaacacgggg gagccgaagg caagttcccc gtggtgggac
     5401 gcaatcaggt gcagcaggtg catgcgcacg tccggggaag gagggtccag cgtcttccag
     5461 gaatccgctt ccggaaggga catgatgcgc ttccacaaat tgttgaccag ttcaatgccc
     5521 agcggaatat ggcccagcag ttctccggct tccgaatagg gcatcgtaaa atcctccttc
     5581 gggtagcaat tttcccacaa cttcccgcag tcgtgaaaca gacacccggc cagcagaaga
     5641 tcacggttca gccccggatt tgcctggcag acggcagagg ccgtgcgcat catccccgct
     5701 acgtgctcca ccagtccgcc acggcgggcg tgatggtagg tgcgggcggc ggcggcgcgc
     5761 tggaggcgtt ccccgaactg ctcaatgaat tcaatgcaca gggcgcgtat gcgcggatca
     5821 ttcatgccct tgatgagcac acaaatttcc ttcagatcag cttcctgctt tttcttcaag
     5881 gttccggaac cggccagaaa gctttccttt tcctgttcgt ccagcaaccg cacgtccaga
     5941 tcggaaactt ccatgccgaa ccctcccttg gcccattggc cgcgcaggct cacgaaactg
     6001 cggaggggca gagaagcgag cgtccggaac cagggtttat cctcccacac cttcagtccc
     6061 attgtccctt ccgcatcggc aaaagacaca tccagatacg gcttgttgcc cttggtcatc
     6121 ttctgcgcgc actgggcaat ctgggcaaaa acttccgctt caatctggcc ttccgcggcc
     6181 agcttgccca actccatgag actgacttgt tccatgcgtg ccatcttgcc cctcttccct
     6241 ccatccgtca agaaaacgcc ggaggactac gataataaaa aaaggaaacc gcttattttc
     6301 ccaatacgcc cagctcggca acggcaacat ggctgccttc caccacccgc ttgcctacaa
     6361 aacgcagata acgtgctttg acaggcgctt tcaggtcaat ccgctgcgga acgggattgg
     6421 cgcggatatt agagaactcc ccctccgcag cgggagcccc ccatgtgttc ccatccatgc
     6481 tcaaatagac ggcatagcgg tcaaccgttc ccgttgcgga atccctgcgc ggcgtataga
     6541 taacggcggc aacgttaacc ggacgcccca tgtcgatctc cagcgcctgc gggggcgcca
     6601 actctccctg ggcggcatgc gtatgccaga gggaggaaga atctccgtct atagccagtt
     6661 ccggagcggc ggcgcttctt tccccggcca ccactttcca gtcccgcgtg gggacaggaa
     6721 taatctgagt ggttacggaa gattttctgc cgcgatattc ggccgccgct ttcaccgtcc
     6781 ccccggcagg gagaaagaaa ggattcctgt acatggcgga ctgcggaccg ggttccgttc
     6841 catccgtggt gtacctgatc actacgttct ccgcggaagc cagggtcacc cgtccattgc
     6901 ggtcataatt caccgccgga gcattcagga tgacgggata cttccataaa gaaacctcgc
     6961 acagggccgg aacggcccgg gattgttcca gcaccacgcg cactccatcc gccgtcacgg
     7021 gccttccttt cagaagaact ctggccccta tgctggcccc ctccgtccat ttgctccact
     7081 ggccgttctc acgcacctcc acacggaatt tgcgcaccct ctgccccagt tgaatgggct
     7141 ctgccagccg gatgacgtca aacgccgcgg gctccggcaa ttttaatacc acgcagggat
     7201 gcttatcttc cggagcggcc acccagtacg tcgtccgttt ccggtccaga acctgttccg
     7261 caccgtaccc cttccaggaa gaggaggact cagcctgtgc gccggaggcg aagttgcggg
     7321 aatacaactc atccagcagc acacggaagc ccgccagaga ctttttatcc tcttcatgaa
     7381 tgcggccgcg gcggtccggc ggcacattca aattcaaatt ggcccctctg cctacgctgt
     7441 caaaatacaa cttcagcaga ttttccggcg tccggacgcg ggaattttca tgctcatgcc
     7501 agaaccagcc cggacgaatg gaaacatcca cctccgcagg aatccagtac ttgccgtcca
     7561 tggtgccttc cgtgcccagc cggtagcgca cggtgccggg cgcgggttcc gtcccggctt
     7621 gcagcggaac gggggtgtat gtggcccagc agggataacc cgcatagccg ctttcattgc
     7681 ctacccagcg gacatccggc cctacgtcgg aaaagatgac ggctccgggc tgccgtttct
     7741 tcaactcacc ccaagttgcc ttccagtcgt aatacgcgct ccggtcgata gaacgccttt
     7801 cccgcgctcc tccgtaatac ccgtcccctc cgttcgctcc gtcaaaccaa gccaggaaaa
     7861 tggaaccgta tcccgtcgcc agctctttca actgctgacg gtacatccgg atataatccg
     7921 gcgttccgta agaggcggca ttgcggtccc acggagacag ataaacaccg aacctgacgc
     7981 catactttcc gcacgcgcgg gacacctcct tcaccacatc gcccttcccc tgcttccacg
     8041 gagaagccgc tacactatgg agggtcgtct ttgtgggcca cagacaaaaa ccgtcatgat
     8101 gcttgcaggt cagcaccacc cctttgaacc cggcgtccgc cagcgtcctg acaagatcat
     8161 ccgcatcgaa atcggaagga tgaaacagct ccggcttttc gtctccatag ccccactctt
     8221 ttccagtaaa ggtattaatc gtaaaatgga caaaggcata catctccatg tcatgccatt
     8281 caagctgctc cgcggaagga acaggcttta acggcgttaa acagcccttc ggcccggctc
     8341 ctgcaaaaca ggccgtgcac agggcccctc ccaatacggc cgccttaatc aactggttta
     8401 aattcattac caacaatcac tatcgtttta actcctcgta cgttcctggc ggtcttattc
     8461 tttccgatca agtcatgcag gccttttagt ctctcttcct ctctgcggca ttgataaact
     8521 tcctttccat tccgaactta ccgccatgcc atacatcgga tgccacctct cctccgcaaa
     8581 aggctatgaa gccatgggcc gggttgccct ctccatcggt gccaatactt ttcagttctt
     8641 tacccgcaat ccgcggggga gcaaagccaa agctattgac gaacaggata ttgcccgttt
     8701 tctggaatta gcgaggaata acggattcgg cacgctgctg gcacacgccc cctacacgct
     8761 gaacccctgt tccgcagacc ccagtgtcgc ccgttttgcg gcccaggtac tgaaagagga
     8821 tctggaactc atggaacatc tgccggggaa tctttataac ttccatcccg gctgccacgt
     8881 agggcaggga gtggaaaaag gcattgaact ggtggcggat caattaaacg acgtgctttc
     8941 gccgaaacaa aagaccattg ttttgctgga aaccatgtcc ggcaagggaa gcgaagtcgg
     9001 ccggaccttt gaagaactgg ccgccatcat ggagcgggtg gacctgaagg acaaacttgg
     9061 cgtatgcctg gatacctgcc atgtctattc cgccggatat gatatcgtca acaggctgga
     9121 ttccgtactg gaacattttg atgccgtgct gggcctggaa cgcctgcgcg ccatccacct
     9181 gaatgacagc atgacgccct tctcctcctt caaggaccgc catgaaacca tcggcaaggg
     9241 tnnnnnnnnn ngatacacaa accttgtaaa ttagtttaaa ctaattccaa tatctggttt
     9301 ccaaggaaaa aaccatccga tttttatgaa agaaatgcgc atcgcgaaaa taacagggcg
     9361 tgaaattctg gattcacgcg gcaaccccac cattgaggcg gacgtttatc tggaaggagg
     9421 cgccgtaggc acggcgggcg tccccagcgg agcttccacc ggggaacatg aagcctggga
     9481 actcagggac ggcgacacaa cccgctacca gggcaaggga gtcaccaagg ccgtagacaa
     9541 catccgtaac gtcattgctc cggccctgat cggtctggaa gccacggaac aaaccacggc
     9601 ggatgccctc atgaattccc tggacggcac attcaacaag gccaaactgg gagccaacgc
     9661 cattctgggt gtttccctgg cgcttgccaa agcggcggcg gcccagctgc accagcccct
     9721 gtaccgctac ctgggcggcc cgaacgccaa gatccttccc gttccgatga tgaacatcat
     9781 caacggaggc gcccactccg acgcgcctat tgatttccag gaattcatga tcgtcccgtc
     9841 cggcgctccc accttccggg aagccctgcg ttatggcgcg gaagttttcc atgcgctgaa
     9901 atccgttctt catgaccgcg gcctttccac cgccgtgggc gacgaaggcg gcttcgcccc
     9961 taggctgaac tctgcggaag acgccctgga ttgcatcgcc caggcggtgg acaaggccgg
    10021 ttaccggttc ggggaggaca tttccattgc tctggacgtg gcttcctccg aattttatga
    10081 accggaatcc gggctgtata ttttcaagaa atccgacggc agccgcagaa ccgccgcaga
    10141 gctgacggac tactatgtgg aactgaagaa aaaatatccc atcgtctcca tcgaagacgg
    10201 ctgtgcggaa aacgactggg acggatggaa aatcctgacg gaaaaacttg gcaaaagcac
    10261 tcagcttgtc ggagatgacc tctttgtcac caacgtggaa ttcctcagaa aaggcatcca
    10321 tctgggcgtg gggaactcca tcc
//
LOCUS       DAWEMX010000091        10267 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2751_length_10267_cov_11.647767, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000091 DAWEMX010000000
VERSION     DAWEMX010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10267)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2751_length_10267_cov_11.647767"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10049..10058
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2004 a         2696 c         3306 g         2251 t
ORIGIN      
        1 cccccccccc ccggcccccc accgcccccg gccccccccc ccccgcggcg gcgggccccc
       61 cggcgccccg gcaccgcctg gctggcggag aagcggatga gcgtgaaaat ttacgggatc
      121 ttctggcaaa aagggcccgg aggggaggca tgaagttgtc cgtggtctgc aaatccagga
      181 agtcaatgcc caggcggcgg aaatcctgcg tccatttctc gcggtgcaga tgcacgacgt
      241 tggcgtgggt ttcccggaca tcctgacggg aaaggttcag gaacatgctt ttgccagttt
      301 ccggatcttt caggatgacg cgtccggcat ccggcagatg cagttccatc gggtctgcga
      361 tgcggatggg gatgaagtca tgcttgaagt tgagctgccc cagggcggct ttattgttct
      421 caccccagaa gtcactgatc atgatgacca tggccgcttt tttctgggaa aggacaagtt
      481 gccgggctac atcggaaatg ttctggtcgg ctccatcgtt tttactggct acgatttcgc
      541 ggacgatgcg cagggtctgc ttgactcctt tggccggggg gatgtaatgg gagtggctgt
      601 ttccgtagat gaggaggccg catttgtctc cgctctgggc tgcgctgtag gcgagcactg
      661 ccgctacttc cgccgcatag tccagtttgg agacgcgggc tgcggaggag gcgtagtgca
      721 tggagccgct gacgtccacc gccagaatga cggcctgttc ccgttcctcc cggaaacggc
      781 ggacaaaagg ggagttcatc ctggccgtta ctttccagtc aatgaagcgg gggtcgtctc
      841 ccggcatgta ttcccggaag tcgtcaaagt ccagcccctg tccgcggaag ccggactggt
      901 attgcccggc gaagttttcc gtggccagat gcctggcgcg cagttcaatg cggcgtacgc
      961 gcttgagaat gtctgaggct ttatccatga aatgattttc cctgtttggg ttcaatcagg
     1021 ttccggcaag gcgccccttg gggcctgaag gataagagcg ggcgcggaag cgtccgcctc
     1081 ctgccgtcac ggcacgggga ctttcgtcag gatgtggtcg atgacgtcgt ccgtggaaat
     1141 gttttccgct tcagcctcat aagagagcag gatgcggtgg cgcaggatat cgtgggccag
     1201 gtccttgatg tcctgtggag tgacgaagga acgatggtgc atgaaggcat tggcgcgggc
     1261 cgccagagcc aggttgatgg tagcgcgggg agatgccccc gcgcggatga gaccgcgcaa
     1321 cttcacgtcc accgtttccg ggaaacgggt ggcgcgcacc agatccacga tgtagccgcg
     1381 cacggcgtcg tcaatataaa tctggtttac caggtcgcgg gaggccgcca cctgttccgg
     1441 cgtggtgacc ggggaggttt ccggcggttt ggcggagctg gccatgaggt ccagcacctg
     1501 gagttcttct tcgcgcgtgg ggtaggtgac cagcaccttg aagagaaaac ggtcaagctg
     1561 ggcttccggg agctggtagg tgccctcctg gtcaatcggg ttctgcgtgg caagcaccag
     1621 gaaggggtcc ggcaggcggt aggtggtttc acccagggtt acctggcgtt cctgcatggc
     1681 ttccagcagg gcggattgca ctttggccgg ggcgcggttg atttcatccg ccaaaatgag
     1741 attggcgaag atcgggcctt ttttggctgt gaactgcctt tcttccggat tatagatcat
     1801 tgtgcccagc aaatccgccg gaagaagatc cggcgtaaac tggatgcggg caaactgtgc
     1861 atgcagcgtg cccgccatgg cttttacgga gagcgtcttg gcaaggccgg gcacgccttc
     1921 cagcaggacg tggcccttgc agaggagact gaggattaat ctgtccacga gcgcctgctg
     1981 gccgatgacc accttgccga cttccgttct gagagcagaa atccacgatg agttcgcggc
     2041 gataatttcg ttgaattctc gggtgtccat gattctgata gcgggtgaga cagtatgccg
     2101 ggcacttacg ttgaaaaaat cctattattt cccaagggca aatccggttt tcaacaggag
     2161 gtcttcccgg aaatggggag ccatcatctg aaagcccacg ggaagttcca tgccgctttc
     2221 cctgacggtt ccgcagggaa tggacatggc ggggaggccg gccaggttgg cggggatcgt
     2281 gtagatgtcc gccagatagg tctggaggtg atccagcgcg gaatctccga ttttgggagc
     2341 gggagcgggc gaggtggggc ctacgatgat atccgccctg gaaaaggctt ccgcgaaatc
     2401 tctggcgacc agggagcgga ctttttgagc gcgggagtag taagcgtcat agtaaccgga
     2461 gctgagcaca taggtgccca ggatgatgcg gcgcttgact tccggcccga atccctcttc
     2521 acgggtgcgt gagaagagtt ccaccagtcc ggcggcgtct tccgccctct ttccgtagcg
     2581 cacgccgtca aaccgggaga ggttggaaga cgcttccgcg caggcgatga tatagtaggc
     2641 ggcgacgacg gcatccgcgt ggggcaggtt gacttctacc agctccgcgc ccaggctttc
     2701 cagctgtttg acggtgtttt gcacagcctc ggaaatgccc ggatggttgc cggaggtaaa
     2761 gtattccgaa gggatgccca ctttcagccc tttgacgtca cggcccagcg cagcttcaaa
     2821 gtccggcacg gggcagtttg cggatgtgga gtctttacgg tcgtgtccgg agatggcctg
     2881 gagcaggatg gcggcgtctt ccactgtccg ggtcatgggg ccgatctggt ccagggaaga
     2941 ggcaaaggct accagtccgt agcgggaaac gcgcccgtag gtaggcttga ggcccacgat
     3001 accgcagtgc ccggccggtt gccggatgga gccgcccgtg tcggaaccga gtgcggcaac
     3061 ggcggtagcg gatgcgacgg cggcggcaga gccgctggag gagccgccgg gaatgcggtc
     3121 cggggcttcc gggttgcgcg tgatgccgta agcggagttt tctccgctgg cacccatggc
     3181 gaattcatcc atgttcgtgc ggcccagcgg gatgccgccc gcctctttca gcaggcggat
     3241 ggaggtggcg tcataaggag cgacataagg ggaaagaagg cgggaggcgc agcgcgtcgg
     3301 ctggcccagg acattaatgt tgtccttcac ggcgatgggg atgccggcca gggggctgga
     3361 aatatccgct ccggccgcgg cgtggagggc tgcttcacgg tcaaaggaga tgtaggcgtt
     3421 ggttgtccgg tccgcctcaa tggcgtccgc cgtcaggttg accagttcgg caggggagag
     3481 ttccttgcgg cgcaggcggt cacgccactg ggccagggtg ccttgaattt ttgacatgag
     3541 aaggaaaggg taaagacggt tgaaaggtga agggaaaagg aagcgggagg atcaggcgga
     3601 ttccaccacc ttggacgtgc ggaattgccc ctgggtctgc tggggggcat tggaaagggc
     3661 ttcttcctgg gtgaagccgg tttcaggaat gtcttcgcgc attacatcca ttgccgggag
     3721 aggatggttc atgggggcga tgcccgtgac atcgtaggat tccagcttgg tgatataggc
     3781 caggactttg tccagatcct tggagaagag agctgtttct tcctccgtca ggtcaatgcg
     3841 tgccagcttg gcaatgtagg ctacgtctat ttggggcgtg ctcatggtgt aatgtagaaa
     3901 agatgcggaa aagaaggggg ctgcctactg ttgttccagc aggcggacga tccagcagaa
     3961 ggaaacggcg atggcgcaga tcagcaggcc cagcagcatg aagttttctc gcagttcccg
     4021 cctcatgttg gtggcgcgtc cgtcacgggc cactacagca cgcagtgccc ggttctggtt
     4081 gccctcccga acgcggtcgg gaggcggcat ggtattgcgg tccaggcgca tgcgggtctg
     4141 ctgggacggg cgtttgagag aggcttccaa ttcctggata cggctctcaa tttcccgtga
     4201 ggcgcgatcc agttctgcca gttcggagga acggcgtttg gtttttcttt taatcatgat
     4261 gcggaagggc tgaaaatcca gatgaaaaag aggatgatga gagcaatcag gagcagggga
     4321 agatggaagg aaagaccctg gaggaactgg gtcatgaatt cacgggagct gatgcgcact
     4381 ttgctggagc ggcgtacccc ggtgagcact ttggtatgct gcatgcgcgc gcactgctgg
     4441 acggcatccg cgaattcctc ctcgcagcgg tcaatgacct gctggccctg gctgatgagg
     4501 ttgtccgtat tttcaatagg ccattcatcc gggcgcacgg tgctgagcat tttcaggctg
     4561 cgcgtcagcg ccatgtggat ttgttccagt tcattgcttt cctggcggag gctttccacc
     4621 tcccggccga tgaggtctgc ggcgttgacc atgcggtcgc ccagctcgtt ctgctggtcc
     4681 ataaacctgg ctttgcgcat atggcattct tccaggcggc ggcgggagtt ttccacttcc
     4741 tccttttcct tccgcaattg ttccagcctc agctgggcct gttccatatc ggcgtccacc
     4801 tggtcgtctg cctgggaatt gtaaccgggg tcggcgtcca tgcggcggta ttgagggtga
     4861 cttggtctca tatgcggaaa aaataatgcg ggccggcgta agggattaca gcgtttccgg
     4921 tcctttttgc cggagcgggg aatccctgcg cgtgctatta tttaactttc acaaaataaa
     4981 tcaagccaag aagaagacag ggaaagattt tccggatgcg ttcccggcag ggaatgccgc
     5041 cctgtttttt cttccggagc cgtgcgttcc ggatatccgg ggggcgacag gacgctccgc
     5101 tttcccgcaa aggaggggga ggtgcgcttt ttttatggtc aagggcatgg atttgctggt
     5161 atgtaggaag catgcagtct gacacggtag aatgtaagcc gaccatgtgg tttaccggca
     5221 gggcggttat catggctctc atgtttctgg ggttcggagt gtatttttat tatgatgctt
     5281 ccatcggata ccctcaaaaa aacctggagt ttttcatgca caaggcgttc gtggacgctg
     5341 gagccgtgtt tgaccgtgag gtgagcggga aaggggcgtc cgcggaggac tgggagcgca
     5401 ttgttctggc gcgcaccgtg gattttccgg agggctacga aattccttcc ggcgtgaatc
     5461 ggaacgcggt gccgtggcct gacattttgg cggattacag tctgatgtcc acccccggca
     5521 agggctggag cgctgcgtgg caatcttatt ccggggaaaa gcgctatccc atcaagccgg
     5581 tggacaaccc gtatgacgcc tccaaaattt ttgaacagtg ggtggccggg tgcatttgcc
     5641 tggcgcttgc catggtggct ctgtttttcc tggtgagaac gcgcggcagg aaaatgtccc
     5701 tgaacgggaa agaggtgacg gccgccggcg tggctttccg cgtggatgac attgcccgtc
     5761 tggatttgcg cagatggaaa ctgaagggac tggccatagc gacgctgaaa ccggaatgcg
     5821 gaggagcccg aatcaggctg gacggcctga cttacggcgg cttccggaag gaggattctc
     5881 cccataacgc ggaagatttc atgaaggctc ttctgtctct ctacagagga gaaatagtgg
     5941 attatgaaga aattcctgat gccggcgggc cgcctgcccg gcaggaggac gaggaacgtg
     6001 ccgtatgagc gaatcccttc caccggaaaa gggcgccaat gaccgtgcgt tcggcgtcgc
     6061 ctgctggacg ctgggtgggc tggcgtttgt tctgttgctg atggtgggcg tttctttcag
     6121 cctgtctgtc cgtaccgtgg tggtggaaaa agtggtggag aagcctgttc ccgttccggc
     6181 acgggagaag ccggagcgtc cggccggagt cgttgaggat ggggcggctg aggagacagt
     6241 gcatatggcg gatgttccgg tatccccttc tgaaaagccc cgttctgtgg aagaaatgct
     6301 ccgggaagcc gatttgtcag atccggctgc cgattctgta ccggtggccg cggccgttgc
     6361 gggagggggg gactccgcta gcggggcatc ccttcctgct gaggatccgg acgaggtcat
     6421 gccccccatg acggaggaag tggcggagct tgtcaaggcg gctcgttacg cccagattga
     6481 aggtgacctc aaaatagcgg tcgtgaagtt ggagcaggcc atgaggctgg aaccggataa
     6541 tccggtggtg ctttattatt atggacttac ctatgaatgg ctgaggaatg cggataagtc
     6601 acgggagttt tttctgaaag tatataccca gcgtgagaag gcggggaaat attttgcgcg
     6661 tgcggcgcgg cacctccagg ccggcttttc cagcccggcg gatttgcggg gggatatgtc
     6721 cttcggcact attttggaat accgggatcc ggaatgcccc gccggggagc gcgtcaagct
     6781 gatggttcct attctgatga aggatggcct gaatgtcagg ccggaggatc tgcgcgtcat
     6841 tgtccagttt tttgacaaag tcaatgggaa gaaagtggaa aagacgcatg cgcccgaacc
     6901 ttcttcccgg tgcgttacgg aacccgcgga ttgggcggac ggtgaggaaa tcatggaaat
     6961 aacctactac atgcctcccc tgacggaaga agagacgatt gcttatggaa gcctgaaata
     7021 ttacggatat accgctaaat tgtattacaa gggggaaccg atggattgcc atgcttcccc
     7081 tcccgtgcta tttttactgg aacagatgaa ccagagttcc ccttccggac tgcccgagat
     7141 ttatgacggc ggtttgcttc ctcctgtaga ggcggctccg gtttctgagt cttatgaatc
     7201 tcttttgcct ccctgatgcc tgatttgtcc aatgtttgat cttccccaca actttggcga
     7261 ttacacgctg gtggcattta ttggccgcac gcgcggagga attctttatc aggccatcca
     7321 gcagggcatg gaccgttccg tgtttctgga gttgctggat ccggacaatc cggagggggt
     7381 gggcgtggag gattttctga tgaaggcccg cacgcgcgcc gccattaatg ctccggtgct
     7441 gggcaccgtc tatgaagcgt cccaggccca gggatactgg ttcgtcacca gcgaacagct
     7501 gggaggctca tccctccagt ccatgctgga ccgcgggcag accctgtcca tgaaggattt
     7561 gctgaaggtg attgaaacgg tcggcaacgt gtgcggcagg tatgagcgtc tgcggaccgc
     7621 tttcaatatc atggagccgc gtcacatttt cctggatgac aagtctgccg tgcggctgat
     7681 gaatacggcc gtgcccggtg actttcacga agaaacatcc cgcgaccaga tgaagcgcct
     7741 gggcatcggc ctgcctcctc tggtgactcc ggatgtgccc ggaaccacgc ggatgcgcac
     7801 cctgctggaa tggatgagag aagggcagaa cggaaagccg atgcaatggg accaggttat
     7861 ggagctggtt gctgcggtcc gcgaacagct ggggctttcc cctcgggtta ccacccaccg
     7921 ctataccgtc cccgtggaat cccgcagaaa agccggaaaa aagctgcttt gggccggaat
     7981 gggcctgctg ggcgtgggga tagccgcggc tgccgttctt cttttattcc cccgggagaa
     8041 ggaaaccgta tcccgtcccc atgttccggc tcctaaacat tatccggatt tttcctccag
     8101 cgatcatacg gaagtccgcg taactcttcc cggaggaggg gaactgattg tgggagccca
     8161 tgaaattacc ctggaatcct accgcctgtt tttggaccag tgggcacgcc tcactccgga
     8221 gctgagggag gaatattccc acccggacca gccggacaag aggactgcct cccatattcc
     8281 ccaggactgg gaggctatgt ggaaggcggc ttccacgccg ggaggcaaat ggaaaggcag
     8341 gaaaataaca ccccgctctc ccgtcgtcaa cgtcactttc tgggatgcct gggcgtatgc
     8401 gagctggaag cccgtggccc ccggagaacc gcgctaccgc ctgcccgagc gcggggagtg
     8461 gatggctctg gggaatatgc tggaaacggg tgagaagggg gacaggacgc tggtgattga
     8521 cagatacagc aatgatcatg atttaaagac cggcgtgtgc ggcatggctt ccggcgtgat
     8581 ggaatggact tcttccatgg aaaaggatcc tgcgcgcgtg aaagagcctc cggggccggt
     8641 ggcctgcggc ggagactgga ggcagcccgg catctccaac cgggtggaat acctgcgctc
     8701 ccgcggtgaa agccgggaca acctgggatt cagaatcgtg cggaatgtcc gctgacccgt
     8761 ttaacgacaa tacttaacat atcaatacca tgaaacatat ccttgcaatg ctgctggccc
     8821 ttctgggggc gtccggggcc ctggcccaga tttacgtgaa agtagtcccg gaaaagaaga
     8881 cgttcctcag cggggaagcc atgtacatga ggctgaccat taccaacaat tccggcgtgc
     8941 cggtggagct gaaaagccgg gaatattcct cttggctgga tatccatgtg gagcattcca
     9001 ctgcgggggc ggaactcccc cagtccaaat ttgccctgtt tccgcctctc aaggttcctg
     9061 ccggcatgag cgtttcccgc aagattgacc tgcgccattt ctatgacttg tcccgtgagg
     9121 gcaactacca tgtgcaggcc gttgtaaaaa tgcccaacca gaaggacatg tttgcttccc
     9181 agaaatccct gtttgccgtc cggacgggaa cacccatgtg gtcccagacc gtggctattc
     9241 cggccagtgc caagcggtgc actttttcca tttgcactat tgccgtgcgg ggaatccaga
     9301 aattgtatgt gcagacgaag gatccggata cgggagtggc ctacaatgcc gtgtgcatcg
     9361 gtgactggct ggggaccacg cgtccgcagt gcatggtgga cggcaaggcg aatgtccatg
     9421 tctttttccc aactaccccc atgctgagcg cgtattcccg catcaattac cggggaacca
     9481 tggaaaagct gcagtactat aaaaaggtgg tgggagcccc ctccatggtc tttcttccgg
     9541 acggaagcgt acgtgtgacg ggagccgtcc attatgatcc taccgtgaag cccaagacgg
     9601 tgccggatgc cgctgatgtt cccggcatgt gaatccgtcc tgtttcgccg gatgccacgc
     9661 aggggaaaag gaaaggcaaa cgtactgctt cttccgtacc gggaaaataa gatgttccgg
     9721 tattttcctg agctattttc ccggctgggg agtgtttttc aggatgtttt gcacgctttt
     9781 ctgcacaacg ctgatgggca gggcgtaatt ttccgcccgg ttcactctgg cgatgttcat
     9841 gccgatgagt tcccccttga gattgagcag cgggccaccc atcagcgtgt accgggaagg
     9901 ggtgtcatgc tggaggacca tagggaaatc atccctgcgt ttgctgaacc cgccgctgat
     9961 caggtcattg gaatcctcag gagcgtttcc tcccagggcc tggcgtctgg aacccagcgt
    10021 gatggtgtag gataaccttt tatccttgnn nnnnnnnntt atccttgcgt ttgactttca
    10081 gtgtcactct ttctccaact tttttcctgg acagaatggg ggaaacggca tccagagcag
    10141 taacgggcgt gccgtccacc gccagaatct catctccggc cttggctccg gcctgggcgg
    10201 cggggccgtc cttggtaact tcctgaatgg tacagggggt gccgaaaacg acgcccaggt
    10261 aggcggt
//
LOCUS       DAWEMX010000092        10253 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2753_length_10253_cov_10.934399, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000092 DAWEMX010000000
VERSION     DAWEMX010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_10253_cov_10.934399"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    327..336
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2261 a         3150 c         2842 g         1990 t
ORIGIN      
        1 gaacccaggc agccgccacg cccagcaaca ccggaagagc caggtattca tgcatccgga
       61 acaggagcga agggagagct tcccatctgc tccaaaaacc ggaaaggggt tccgcgcagc
      121 cgggagtggc ggaatgcatc cgcgcggaaa tgccgggcgc aagcagcatg cacagggaac
      181 ctgccacctg gaacacaagg cccgcataaa accacagggg aagtttttcc ttccgcacca
      241 ggcggacaaa aacaaacaag gctcccagca aaagccaggt tcccggaata ttgttttcat
      301 tggtcatgcc tgccggaaat cccagannnn nnnnnngcca gtaaatcgtg acgccgggcc
      361 gggccgtgca cgtgaacagc aggagaagcc ccaatccgaa cagacgcacg tccggccagg
      421 aacggggatc caccctgcgc ccggaagcca gataaaacat catcagcgca agaccgacct
      481 gtacaaaggg gttgagcagc atgaagagcc attttcccgc cgtcgctacg gcaaaggcca
      541 ggtattcccc gattctggga ttccaggtca tgtaggaggc tacgcaacgc tcccagacca
      601 ggctgaaaga gaagcagtgc tccatgcccg tcagggcatg atggtacgta tcggaagtga
      661 gaggactcca ccagctgagc agcgccgtat acaataaggc ggctgcggtc agcgcccaaa
      721 accacaccct ggacgtgcgg ggggacatca tcagctcgga tccggtttcc ttttccgcag
      781 tgcgggaggc agatccaggt cttccccatc cagaatgagg ggacgcacac gggcaaaaag
      841 gccgtggccc gcacgctctt cctctccttc ctccggaggc aagccttcct ccagctcctt
      901 gatgtcaggc atcgggactg actccaattc cggctccgcc tccgtttcgg aaatttgcgc
      961 aacagacgcg gactcccgga gcgtctcctc ttcatccgga atcccgggaa caggccttac
     1021 gggcaccagt tcctcttcca attcacgttc atccctcctg gggacgggaa caggggcgga
     1081 aagatccggc tccggatgcc gttccggttc gtcaaccggg gcaggaggtg caggctgaat
     1141 aatggttttc ccctctccga ggaaagattt ttcctccggt acaagcactg tctgcgacgc
     1201 cacttcccgc tgaagcacaa cagacgggga aacagatgtc tctaccacag gcacggcgcc
     1261 cagcacggtc agggacaggc ggtcttccgt ctgctgctcc acggaagcgc cgatgtgaag
     1321 gcgcaccccc ttccccagga tttccgccac cccgtccacc agggcttcca ggcgttgcag
     1381 tgtcagggaa gctccgccgc gcaccagaac caggacatca tccacttccc ccaaacgttt
     1441 ttccagaaag agaggggaat gccggacctg atccaatatg tccgccacgg aagcatccgc
     1501 agacgcttca cccacgccaa aggagcaaat tccttttccc gttccggcta cgctgagcag
     1561 gtcgtccaat cccacccgga gcaggtttcc ggaagaattc aacagagacg gcacaataag
     1621 cacggcacgg gctataaggg cgttaactac ggaaaaggct tccagcactc ccctgtcggg
     1681 gtctatgaga ccttccatgg cgtcattgtc aaaccgcagc accatgtcgg aatacagggc
     1741 aaggcgggac agcgcttcat ccgcctggac agcgcgccgt tccccttcaa acctgaaggg
     1801 acggatgaca acgctgacga cataagcccc ctgttccttg gccagacggg ccgcttccgg
     1861 agccacgccg gaccctgttc ccccgcccag cccggcgaca aaaacagcca gggcgctgcc
     1921 ttccagagca cggagaatgg aagactcgct ttcacaggcc gcctgggctc ctacggaggc
     1981 gtctccgccg gagccgagcc ctctggtaag cctggcgccc agatggacct tgcacggaac
     2041 ggctgaagcg ttcaagagcc gggcatcaag attcatggca caaacggaag ctggctgagg
     2101 ggacagcaac aacacttctt ccattacctt ggtgcctgcg gcccctatgc cgcacaggca
     2161 gatttttccg ggcaggccgg acggcccctg gggagaaatt tccagcatgg ttgttaattt
     2221 ggataaaggt tgatcaggag ttcttctttc cgaagaaacc cagcatccgg cccagccagc
     2281 tgcgctgttg cggcagctcg gcatccagaa tctgggcata ccgtatcagg cctatggccg
     2341 tgcagtagcg cggatcgtcc aggtaggaag gcgctccgtt tttgaccagc gtaggccggc
     2401 tgatggccac gccgaacacg tgggaggcca gttcccccac gccgcgcatc aggctggcgc
     2461 ccccgcacag gtacacgccg tggcagcggt tgcccttgaa agtatccttg ggcagggagg
     2521 atttgaccag attgaagatt tccaacagac gggaacggat gatttcattc agcacgttgc
     2581 gttcgatggc ggcatccttc atatgcccct ctccgcgcac gcgcaccatc tcgttggttt
     2641 tcccggaaaa gctgttggcg tccccttccg ttttcttgag cagctccgcc tgggccagcg
     2701 gaatgccggt catcagcgtg atgtcattgg aaatgtggtc gccgcccagc ggcacgcatc
     2761 cggaggccac aagctggccg tccaggtaaa gcacatagtc cgtcgtgccc gctcccatgt
     2821 caataagcag agcgcccgcc tgcttgacct gcctgttgag tacaaactgg gccgtggcaa
     2881 tgggggcaaa aaccacatct gcaatatcca gcgggacttc ccgcacgcaa cggaaggagt
     2941 tggtgatgcg ggatttgatt ccgtggatga tatggcagtc gatgtccagg gttcttccgg
     3001 tcaatccggc gggattggtc aggttctcct gcccgtccac ggaaaagagg ccgggcacgc
     3061 ggtgcaggac gaattgttcc ggccccaggg cgacgtcccg ggcaatttcc gtcacctcgt
     3121 ccatatgctc ctgggaaata atgctttcgt ccggggggag acggaacgtc ccccggttgt
     3181 tctgccccac aatatgggct cccgtcacgg acagatagac ggtcatgata tccacgtccg
     3241 catggtcctg ggccatgttc cacgcatcgt aaatgcactg gatgaccttg gaagtgtctt
     3301 caatctctcc gcgaaccacg ccctcgctag gaacctcacc cacgccgatg attgtcacgg
     3361 tcgcatctgg cttcacttcg cctacgacca tgcaagtctt gcttgttcct atctcaagcc
     3421 ctacgtgaat ttttgtctta gccatgaaag tcaaggaact atcaacgtcc tcgcacctga
     3481 tagcacatta ctttccatta ttcaagaaag cctgcgcgaa catacgcaga aaacgcggtt
     3541 ttcctccgcg cagccccggt tcaaaggcct gtaggatggc gcaccaggaa gtttctggta
     3601 ttgcggccgt ccactctcca gcggggacct tcgccgcggc gcccgcttcc cgtagtgtca
     3661 aaccggaggc cgcagatgac catgaagacg tgtccgtttt tggcataaat ggaaatccag
     3721 tccccggcgc ctttctgccc gtaattcagg aaaagcctgg aatggcgggt ggacgtgagc
     3781 aaaccggctt ccttcagcac aaaggaagtg ctgccggaac agtcataatg agtatcataa
     3841 tgcctcctgt gccccccgcc cagccggtac ggcttgttga ccagggaatt ggctgccgca
     3901 atggcgcgct tgacctgcga aggggcattt ctggggggaa tggctttccc gttgtgcagc
     3961 acggccgtct tgccgggcac aaagtggtag gcgggaagac gcgcaggagc ttcaacttgt
     4021 tgctgggagg aacaggaaga gagaaaaagg ccggccaaaa gggcaccagc cacggagaga
     4081 gttctgtggg ccgcaaatgt cataagaatt ataaactgcg gaattcccgc ccccttggca
     4141 agaaaataaa tttcccccgt ccacattaca tggtagacgg gggaaagaaa tcagcaatat
     4201 ttagccctag gaacggctgt gggcatcctt ttccacagcc gcccacaggg aatccaggga
     4261 ggcctggacc tccgtcaatg cttccttcac aggggcgccc ggtttcccgt gcccgaagag
     4321 gtagtattta atcttgggtt ccgttccgga ggggcgcacc gcaaaactgc ggccgtccgc
     4381 cagatccaca aaaatcattt tttccgccgg aatgggatcg ccttccgcgt ccaccaggtc
     4441 gcctctggaa aaatcccgga tgccgctgac ggctacgccg tccacttccg tgggaggatt
     4501 ggaggaatag gaggcggcaa gggcggcgat tctggccgcg ccgtctgcgc cttccatcac
     4561 cagtgatttg cccatttcca aatacacacc gaactgctca aaaagttcat gcagcagttc
     4621 cagaaggctc ttgccgatgc tttccgcata agcggccaat tccgcaaaaa tgatggcggc
     4681 ggagttggcg tccttgtccc gcacaaagtc ctgggccagg tagccgtagc tttcctcccc
     4741 gccgaagaca aagtaacggc tgtattccag gcgcagggca cgcgtttgtt cctcgctcat
     4801 cctgcggtag ccttcccgtt tttccgccgg aatagcctgt tcatatttac cgagtttctg
     4861 ggcaatatac ttgaaccccg tgagcacgtt gaccacttca taaccgaagt ggtggccgat
     4921 ggcatcctga agccccgtcg tcacaaaggt tttcaccatc acggcacggg aacggttgga
     4981 gtcattgatg atgccaagat cggacatgct catgcagcgg taccacgcca gcagggagcc
     5041 gatctggttg ccggtcagca ggtgcatctt gccgtcttcc ccgcgcacag ccacccccat
     5101 gcggtccgca tccgggtccg tggcgatgac gatatccgcc ccagaagcat ccgcctgttc
     5161 aatggccagg gccagagccg gcggattttc cgggttgggg gaagcaaccg tcgggaaacg
     5221 gccgtcctgc acatcctgcg ccgccacggt ctgcacgttg cagcccagtt ccttcaacag
     5281 gggcacgatg atatggccgc ccgtcccgtg gaggttggaa taaacgatct tagcccctcc
     5341 cctgttgaaa agctccggac ggagcagcac ggttttcaag cgatccatgt aaatacggtc
     5401 aaaggaggaa tccagcacat gaagggttcc gcgccggtct tccggcaacg gttcatattc
     5461 ttcggaagtc agggaattga cctccttgat cacggccttg tcatgcggag gcacaagctg
     5521 ggccccatca ttgaaataag ccttgaaacc gttgtcatgg gaggggttgt ggctggcggt
     5581 cagcaccacg ccggaatccg catggagttc ccggatagca aaggagattt caggcgtggc
     5641 gcgaggactg tcgaacaagt aaatgtcaca acccaggtcc gtgccgattt ttgcgcagaa
     5701 ttcggcaaag tcacgggaaa agtggcgcgt gtcgtgtccg atgaccaggc ggggcttcct
     5761 gcccgggtcc gtagcctcca catgcttttt cacataaata atcaggcccc gcattgcccg
     5821 tcccacattg aagtagttca tgcaggcagt tcccacgcag ggatattccg ggcggccgtt
     5881 cacgccgccg ttcccctgct cggcccgggt tacgatgctg ccgatggtgc gtccccgcag
     5941 cccccccgtt ccgaaggcca gcgttttgta aaaacggtcg ttcaactcct gccattcccc
     6001 ggcggcagcc agttcctcta cggcctcgcg tgctatgggg gatttggttc catccagcag
     6061 aaggcggata ttggtccggg aagactccaa aagcttccct tctttaacgg cattcatcag
     6121 ggattcgtcc agtgtattca tgctggcgga atagtacatc atgccccttg tttgacaata
     6181 gctctttcca tcatggcctg agaaaaaaac gccatattca tcccggtgcg ggcgcgggga
     6241 acgctttcct tcccgtcccc tttttattta aagggactgg cggtcataca gctcccggta
     6301 caacgggcat gaggcataaa gctctgcatg ggaaccgtcc ccgacgatgc gcccgctctc
     6361 aaacaccaga atgcgcgtcg ccacgcggat ggaactgaaa cggtgggcca cgataagcgc
     6421 cgtccttccc tccaccagcc ggtccagttc ctgctggatc agggcctcgc tttccgcatc
     6481 aagggaagcg gtggcttcat ccagaatcag aatgggagca tccttcagga aagcgcgggc
     6541 aatcgctata cgctgccgct ggcctccgga cagtccgctt cctccgtcgc caagcatggt
     6601 ctggtagccg tccggcatgc ccatgataaa gccgtccgca gctgcggcgc gcgccgcagc
     6661 caccacctca tcatccgcag cgtcctgccg tcccagccgg atgttttcca taatggtgcc
     6721 gtggaacagg acggggtgct gggaaacgaa ggcgatattt ttgcgcacat cgtgcaggga
     6781 agcgtccctc acatccacgc catccacgcg cagggttccg gaactaacgt cataaaacct
     6841 ggggagaagg ctggcaaatg ttgtttttcc ggctccgctg ggccctacca gccccactac
     6901 ttccccgggg cggatatgca cgtttatgcc gtccagcacc ttgcgggcac cgtcatagga
     6961 gaaggaaacg ccgtcatagt ccagacggcc ggcggcgcga cccatagcaa cggggtcagc
     7021 ggcctccggc atggtatccg gttctttcag gaggtaattg atgcgttcca gggaagcgta
     7081 agcgctcttc aaattggtga gcgtcacacc cagccgtttg agagggtcgt aacacatgaa
     7141 cagggcggcg gccaaagcag taaaatcagc cttggtgatg cccatttcat gcccccttac
     7201 cagcaaaacg cccagcccca gcgccgtcac catttccagc acgggaacca ggaagtgcct
     7261 gtacttcacc gttctgagat tgattttgaa aaaacgctga atcagcccca ggaaaagatt
     7321 tacctgctgt tcctgcattc cgtaggaacg gatttcacgc tgggaggcca aattttcctg
     7381 ggctgcggcc gtaatattgc ccagttcgtc ctgagcctgt ttggacttct tcatcacgcg
     7441 gcggccgaaa aagcggatgg gccatatggc cagcgcaatc agccccagat tcatcaggac
     7501 tactgccgta ttcggatcaa caaatacctt gtacaccagg aaggcaaggg cggtcagcag
     7561 agtcaggggc tgcttgatga tgtcattggc cgctaccacc agccccccct gcacgttggc
     7621 ggcgtcatta attaggcggc tgatcaggtc cccccgtttc tggcggtcaa tgaaggaaag
     7681 gggcagcgtc tgcaatttgg aaaaggcgtc cagcctgatc gcctccagca ctttcatact
     7741 gacgatgctg atccagtaca cgttgaagaa cgtgcctatg ccacggacgg cgaagaccag
     7801 aggcagcagg gaacatacgg ccagcagcac cgccaggtgc atgtgttccg gggccaccag
     7861 ccgggcgatc atgtcctgaa gctccggggg aatctgtgta ttgtcaaaca ccaccggaaa
     7921 tactttggca atcatcagcg gaaagccgaa accgctggcg gcggcagcca gagccccggc
     7981 agccagagcg gcgatgaaca gcttcatcac gggcttcaaa taccgcgcat attcaaaaaa
     8041 ctggcttaac atggagcgcc tccttcctcc tccgcaacca tctgctgctt attgtaaagt
     8101 tcccggtaca ggggagaaga agccatcagt tcctcatgcg tgccgtccgc aaccacacgg
     8161 cctttctcca gcaccaaaat gcggtccgcc atccggatgg tgctgaaacg gtgggcaata
     8221 atgaacgtcg tgcggccgga agccagtctc tcaatctcct tctgaatctg ctcctcgctc
     8281 ttcatatcca gggaagccgt ggcctcatcc agcaccagga tgggagcgtt tttcaaaaag
     8341 gccctcgcca gggaaacgcg ctgccgctgg ccgccggaaa ggccctcccc catttccccg
     8401 atcatgcggg aatacccttc ccccgtctca ttgataaaac tgtccacggc ggacatgcgg
     8461 ccggcttcct ccacctccgc gtcggaagcg tccggacggc cgatgcgtat attgtccgcc
     8521 acggagccgc ggaacagaac gggatactgg gaaaccagcg ctatgtggga aagaagttcc
     8581 tttttactca tctccctcac gtcgatgccg tccaccttca cgctgccgga aaggacatca
     8641 taaaaacggc acagcaaatt aatgaaagtc gtcttccccg ctccgctggg accgaccagg
     8701 gccaccacct gcccggacgg aatgcggaga tcaatgctcc tcatgacagg agagtcctgc
     8761 tggtaaccga agcaaacgtt gcggaactcc acttccccct tccatgccgc cgggctcctt
     8821 gggttttccg gttcgggaac gtcatcagga gcattcagca cgtggttgat gcgttccaga
     8881 ccggcattaa gggtttccgc cttgttatgg acggccccca gcttcttgac cggctcatag
     8941 caaagataca tggccgtagc cagcgcggca aaatccccca tgttcatgcc gttcatgttg
     9001 ccaaccagca gggtggcggc catggctatg gcgctgacga actcaatcaa aggggtcagc
     9061 aggctctgcc acctgaccgc gccgatggat gcctggatgt aacggcggat cctgtccctg
     9121 aacagcctta tctgctgctg ttccagttca aaggcccgga tatcccgctg ggaagaaaaa
     9181 ttctcctgga gcgtggagga aaggtccccc tggctggact gcactacccg ggcgcgggca
     9241 agcattttcc tgccgatgta ccggatggga atcacgcaaa cgcctaccag gagcatattc
     9301 gccagcagaa taagaaactg ctcgctgacg gaggccctgt aaatcagaaa tcctaacgcg
     9361 gccaaaagag tcaggggctg gataataagg tcattggcaa tctgtaccag gccgccctgg
     9421 agaaactggg tatcctgcat caggcggctc agcaggtccc ccttctgctg ccggtcgtga
     9481 aaagccaggg acaaatcctg atactttctg tacaccttga gccgcaatgt ctccagcact
     9541 cccagcccta ttttggaaag caaatagaca ttgacaaaag aagacaggcc gcgcaccacc
     9601 accaccagcg ggagcatggc cgccgtggcc cacaccgtca ccaggggcag gttttccggg
     9661 ctcacatacc acaagagcac ccgttccgcc cacggaggca gcggtttccc gtcaaaaatc
     9721 acgggaaaca tctcctgtaa aatcaccgga atgccgaaac cgctggaaac agcggccaca
     9781 atgcctccga ccacagccca caccagcgta cccttataag gaagaagata ctcgcgccac
     9841 aatgccctgg cgcgctggat ggaggattcc tgcccctctg ttgactgttt cctcataagt
     9901 attccggatg gccgcgcccg ggaacggacg gcgcgcaatc ggcagggatc ataccatgaa
     9961 gaccgtgaat gacaagctcc ttgcgataaa aactgcatgg gtaaatgcct ggcgggcaga
    10021 cttccgggca tcccgacttg cccgccaccc cgtaatggtc tacctttccg tcgcatgctg
    10081 aagacaggat taatccaacc ggaaaccatt cccggccact ttccccgaaa tctcagaacc
    10141 atcgtggaac tgtaccgttc ctgcctggac gcaggcgcgg agctcgtcct ctgtccgccg
    10201 ctggccctca gcggcgtgca taccggggaa ctggctctgc gcagcggctt ccg
//
LOCUS       DAWEMX010000093        10222 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2763_length_10222_cov_10.619849, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000093 DAWEMX010000000
VERSION     DAWEMX010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2763_length_10222_cov_10.619849"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4156..4165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5649..5658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7014..7023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9388..9397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2045 a         2639 c         3119 g         2379 t
ORIGIN      
        1 actggaaccc ttccggtgta acaatttttt acgctgcgcg acgatgagga agcaactgtc
       61 tggtatttta ttcctggttc aaggaaacct ggtaatgaaa aaactcccga cagccgatgc
      121 cgccgggagt ttttacagag cgtcttaaga aggaatcttc tttagaaaca gaggccttcc
      181 ttggcgttgg cccgcatgaa gtcgcggttg aggcgggcga tgttgtccac gctgatgcct
      241 ttcgggcagg cggcttcaca ttcatactgg ttcgtgcagt tgccaaagcc ttccgcttcc
      301 atctgcttca tcatggccag gacgcgcttg gaacgttccg gctggccctg cggcagcaga
      361 ttgagatggg cgatcttggc ggaggtgaac agcatggcgg aggcgttttt acaggaggcc
      421 acgcaggcgc cgcagccgat gcaggcggcc gcgtcaaagg cggcgtccgc tttcactttt
      481 tccacggcga tgttgttggc gttcggcgcg gaaccggtac gcacgtcaat atagcctcca
      541 gcggcgatga tgcggtccag ggcggagcgg tccaccatca ggtcccgcag gattgggaaa
      601 gccttcgcgc ggaagggttc aatccagacg gtatcgccat ccttgaactt gcgcatgtga
      661 agctggcaag tggtcacctg gcgttccggc ccgtggggaa tgccgttgat ggtcagggag
      721 cacatgccgc agatgccttc ccggcagtcg tggtcaaaat ggatgggttc cttgccttcc
      781 ttgaccaggc cttcattgac gatgtccagc atttccagga aggaggcgtc caccggaatg
      841 tccttggcgg cataggtctc aatgcggccc ttggcttccc ggttttcctg gcgccagacc
      901 ttgagagtga gattgagtgt tttagccatg ttataatctt tcttttgagt gttaatgtat
      961 tggctatctt tacttgtagc tgcggatggc aaggtgcacg ttctcgaagg tcagcggttc
     1021 cttgtggaga acaggcaggc tgtccacgcc agtgtattcc caggcggcta cataggcgta
     1081 ttcatcgtcg ttgcgcttag cttcgccgtc cggcagctgg tattccacgc ggaagtgggc
     1141 gccgcaggat tcctggcggt tcagggcgtc atagcagagc agctgggcga attccaggaa
     1201 gtcggccacg cggcaggctt tttccagttc cgcgttgatg ccgtccgccg tgccggttac
     1261 gcgcacgttt tcccagaatt ccttgcggag ctggggaatg cgttcaatgg cgtgggtcag
     1321 gctttcttcc gtgcgggcca tgccgcagtc ttcccacata atgaggccca gttcccggtg
     1381 gaaggagtcc accgtcttgg tgcccttcac attcaggagc ttgttgatgc gttccttcac
     1441 ttcgtcttcc gcagccttga attccggggc ggcggtggtg acggcgccgg gtttggtggt
     1501 cgtcaggtaa gcgggcagcg tggcgggaat gacgaagtag ccgtcagaca ggccctgcat
     1561 cagggcggag gctcccaggc ggttggcgcc gtggtcggag aagttggctt caccgaggac
     1621 gtgcagcccc ttcacattgg acatgaggtt gtagtccacc cacaggccac ccatggtgta
     1681 gtgggaggcg gggtagatca tcatgggcgt ttcatggggg tcgtcgtccg tgatttcttc
     1741 gtacatgtca aacagattgc cgtattggcc gtcaatccat tcgcggccca tgcgcttgat
     1801 ttcatccgca aaatctagga agacgccctt gccggtgacg gccacgccgc ggccgtcgtc
     1861 gcaggcttcc ttggcggcgc gggaggaaat gtcgcgcgga gccaggttgc cgaaggaggg
     1921 atacttgcgt tccaggtagt agtcccgttc ttcttcggga acatcggaag gcttgatttc
     1981 ctttttacgg attttttctg cggtttcacg ggacttgggc acccagatgc ggccactgtt
     2041 gcggagggat tccgacatca gcgtcagttt ggactgatag tcgcccttga ccggaatgca
     2101 ggtggggtgg atttgcgtga agcaggggtt ggcgaagaag gcaccgcgct tgtaggcctt
     2161 gtaggcggca gtgacgttgc agcccatcgc gttggtggac aggaagaaca cgttgccgta
     2221 gccgcccgtg cagagcagga cggtatcccc tgcgtaggat ttgatttcgc cggtaaccag
     2281 attgcgggtg acgatgccct tggcttcgcc gtccaccagc accagatcca tcatttcatg
     2341 gcgggagaac atttcaatgt gtcccttggc cacttccttt tccagggcct ggtaggcacc
     2401 cagcagtagc tgctggcccg tctgtccgcg tgcgtagaag gttcgcttga gctgggcgcc
     2461 gccgaaggag cggttgtcca gcaggccgcc gtattcacgg ccgaagggaa cgccctgggc
     2521 cacacactgg tcaatgatgt tggaggagac ttccgccaga cggtagacat tggcttcacg
     2581 ggcgcggaag tcgccgcctt taacggtgtc gtaaaacagg cggtaaacgg agtcgccgtc
     2641 gttctggtag ttcttggctg cgttaatgcc gccctgggcc gcaatggagt gggcgcggcg
     2701 ggggctgtcc tggtagcaga agcatttgac gttgtagcca agttcggcaa gcgtggcggc
     2761 agcggaggcg ccagccaggc cggaacctac cacaagcacc gtgtacttgc gcttgttgtt
     2821 cggattgatc agtttggact caagtttgta cttggaccat ttgtcctgaa tagggccgga
     2881 gggaattttg gcatccggca tccagtcgct tacgggaaaa ttaatcatga ctttgagagg
     2941 agttgatggt tatttgatga tacccagaag aaccgagacc ggcacggaga tgaagcccag
     3001 gcagataatc gcgccgtagg cgatggcgat aaagttgtag aagggccgga ttttgcggga
     3061 atcgacgccc acagtctgga agatggactg taccccgtgg cgcagatggc tgaacagcat
     3121 cagaacggcc aggatgtaga aggcggagca ccatacgttg gagaatccgg tcacgatcat
     3181 cttgtacacg tcaaaggttt ccaccgtacc gtcggaaatg aaggtggtga actgggcggg
     3241 gtcgtatccc acgcgcaggg tgtactggta caggtggtag atcaggaaca ccaggacggt
     3301 caggccggta tagatcatgt agcgggagga gagcgtcgcc ttgatggtgt tcttgaacac
     3361 gtagggttcg cgggccgcat ggttttccag cttcagctgg atggtcagcc agacgtggat
     3421 gatgaagatc accgcaagcc ccgcgcggat tccccagagg gctgcttccg gcatggagtg
     3481 caggccgtgg gcgtaggtgt tgatccattc agggcctccg aagatgagaa ggttgcctgc
     3541 caggtgtccc gcaaggaaca agaccaaaca taaccccgtc agagccacaa tgattttgcg
     3601 gccgatggag gatgtcacga atgtacatat ggtttttact agtgcattca taggcgctct
     3661 aatgaaataa atgcgtcttc acaggatgcc tggttcaagc ccggatggca agcctcttct
     3721 tgaatttccg gcgcttttcg gatttttgaa aagccgggga aagatgttgc tgtttatcag
     3781 tttggaatgg ttatcatttt gttgcctgtt ccggcttgac gaggctttcc gcataggcgt
     3841 atcgtctgtg catgaaaaga agtaaaaagg aagtgctgga cgcggatttc gcagcggcgc
     3901 gcgccggcgt tctggatgtg gccgcatttc tggaccgggt ggaccgcggg gaaggggagg
     3961 cggatttccg ctaccatgcc ctgatggatg ttcttcccct cttatccgca tccggccccg
     4021 gacgcgtccg ggccatcctg gaagcccttt cctacaggga tgacaccgtg ccggaaaccg
     4081 ccgcagccaa agcggcctgc ggagccccga acctcagcaa ataagaatga tggctttcat
     4141 agaacctcat atacannnnn nnnnntacat atggtgtgcc gcaccacgga cgaataccgg
     4201 gccatggcgc aggccggctg cctggcggta ggggaaccgg cgttctgggc cggttacgac
     4261 cgttcgtccg cggacggttt ccgggattat ttcgtacagc ttacggaatg cgaacccgcc
     4321 agggcggcca agttcggcat ggaccattac acatggctgt gcatcaatcc caaggaggcg
     4381 caggacgtgg ctttcgcgcg ggatgtgatg aagctgattc cggagttcat cgaccggccc
     4441 aatgtcctcg gcatcgggga aataggactg aaccggaata cttcccatga aatgactatt
     4501 tttgaagagc atctggatct ggcggtgcgc ctcaaccagc ttgtgctgat tcacactccc
     4561 catctggaag acaagcttaa ggggaccgta atgattcttt ccgccctgaa aaaccgtccg
     4621 gatctggacc cttgccgcgt gctggtggat cactgtgagg aacatacctt cccgctggtg
     4681 aaggaggccg gttactgggc cgggctgacc ctttatccca ccagcaagct gaatcccaag
     4741 cgggcggcgg atattctgga attgtacgga atggaccgtg tctgggccaa ttccgcggcg
     4801 gactgggggg attccgatcc cctggcgctg acggccctgg cctgcgagct gcgcaggcgc
     4861 ggtttttccc ggcaggaaac ggaacgtctt ctcctggaaa atccggagac attcatggga
     4921 cagtccccga agttccggaa agtgtcgtcc cgttaattcc gcccgcggcg cggcggaggg
     4981 tacggccctc tactccccgt ccgggaaaaa gacggtgagg acggcgccca ggatgaccgc
     5041 cgcgtcatcc agaaggccca gcctgccgat tccgtcaaac aggaattcgt cgggagtcag
     5101 gatgtaggcg ccggcggcgg ccagaaggca tatattccgt ttgttgaaaa aacgcggttc
     5161 tccttttctg cttttgaaat accggaacag gcgcagtagc agctgctgtt tttgaaggtt
     5221 catggatggg aacgggggat aaaggttcaa gttcagccat tttcctactc cttttccctg
     5281 tttcagggaa ggagtctgtt gccgacggac ggaaaccgca gcgatccgga ccgtgcgggg
     5341 aaatggattg ctgaaaggat gggctgttcc ggatgctgag ggaagccggg ctttgcatgc
     5401 tccggttccg gctgtcccgg catgggaaaa aggggcgtgt acggaagtgg ggcggggccg
     5461 gaagctcttg aaatgaccct cccgggggag cctgaatgcc atttccccgt caagtgctgg
     5521 ccgtgagggt acccagcaaa tagaagcttc ttctccctgt tttttccggc agatgtttga
     5581 acggataagg aaaaggccgg ctcacgattt tgtgagccgg cctttgaata tagggaggcg
     5641 gacggtacnn nnnnnnnnat gccctgcggc gacgcatcat cagcgcggcc agtcccagca
     5701 ggcccaggga agccgtggcg ggttctggaa tgctggcgat ttcactcatc agctgaccaa
     5761 cttgttcctg ggaaaggagg gagtcgtgga tgtagagctg ttcgatggat tcagccagtc
     5821 ctgcgtccat gagaatctga ttaatttgct ggttgccgaa tttcaggccg ctgttggaat
     5881 agtacgtagc gctgtctcca aaatagatgc gggtgccaag atctccggtg actacggaaa
     5941 tggtcaggga accttccgtg cccagcgggg tgtcaacgac gggcccgctg tatgaggtgt
     6001 tgttccataa tccctggagt ttccggtccg tgttcaggcc cacgccccag cttacactgc
     6061 tgtttttggc gttaaacaga gcggtatagg tctccggagt gttgattttg gacaggtcaa
     6121 gaaccatcgt cacagacata acgtcgcggt tgttggtacc gtatccgttg aaggttatcc
     6181 cggacaacgt cagattgctg tcgtcagacg tgatggaaac ggaatctttc cgggtgccga
     6241 aatccgccgt ttgggaggca aagtccgaat atcccacgat ggagctatcc aacagagcgg
     6301 tagccgcggt agcgcttagg ctcatgccgg ccgaaagaat cagggtggat aataatttat
     6361 tcatggtcag tatcttaata aaatgattca aggttcaatg tatgtacttg tgtttgaata
     6421 acgaatttgg aatccgcaag caattaaaaa tattaagagt tagtatattg tatttataat
     6481 tcattgaaaa taatgatttt ttaatatctc tggtcctttt gctaagatga cgggtcagtc
     6541 tttgttattc attggtttca tgattgattt ggcctctttt tcattacttg acggattcca
     6601 aatccgattg gggaattcca aattcgctac aagttgccat ccaaaaggaa taataaatat
     6661 gaagaaaaca ctgacatttt taatcgcgat gggaagcttt gctgtttcct cggaggcagc
     6721 gaccgttttt aattattaca ctcccggtac ggataattac aatagtacca tccatgggtt
     6781 ttatcttggc ctggggtctt cttccctggc tccttccgat tcctcttctc cctcctccct
     6841 gtccggagag atcagactgg acagttttac gctgcgcgga gccgccaaca atttgggcac
     6901 taatgtcact tacggcttcc tggtgctgaa tggctccgac aattcggtca tcggctggag
     6961 caccaatacg ggaactactg cagcagggat taatctgacg ttcagttttt cttnnnnnnn
     7021 nnnatggttc tcccctgctt cttgacgcgg cattgacata ccgtttcctg gccgtgagcc
     7081 agtccgtgat ggacttgatg caggatacgt ctaaaacgta tttatatgct gcggggggaa
     7141 cagccgcccc tactgctaac acggaaggag ataccgtaac gatcagcggc ggattgactg
     7201 ctcccggcat ccgcggccat tatgacgccg agggagcgcc tggtgattct gtcgttatta
     7261 cgggagccaa cggaaccaat actttcagct tattatcccc gattttcacg gaaatccgag
     7321 gggaaatggt gccggaacca gccacggttt ccctgggcct gctgggactg gccgcgctga
     7381 tgatgcgccg ccgcagggct tgagcttcct gatggcgaaa aatcggcgga tgagagctgt
     7441 tcttgcggaa cagctcttgt tttttatatc cagagaggaa gaattgctta tccgtatgcg
     7501 ggattcatgc cctccggaag gtacggaatt tccgggggca gggggccgta tccggttttt
     7561 tccgcatggc ggcaatttgc tggaaacgtt tttgagacgc agtaccggaa agctttttct
     7621 ttaccggaaa atgccgtcaa attcggcagc gcgcttttcg tccatatggt acacagtagc
     7681 tttcacatag cggcctttca ggccggatag ggatcccctt tgcagctcac agacctggag
     7741 ggccgccgca tacctattat ccgccgtcct attccccggc gctggtattc cggaagaacg
     7801 gcaatgggcc ccatgcccag cacttcatat tcttttccat cgtctgccct gatgacggac
     7861 gttgcatcaa cgatgttccc gacgatgcgg ctgccgtgga tggcgactgc atcctgctcc
     7921 gggatgaagg aaggactgac gcgcatgata tggagcaggt aatgttcatt gcagccgggg
     7981 acgaaatagt ttcagaacgc ttctctcgtg acatgttccg tttcggcatg gtctgaggat
     8041 tgttccgttc ttaatgcaat ggtcatgaaa gattgttctt tacggctccc ccttggagaa
     8101 attccagagg cggtgcatcg tcataatgtc cggaatgttc atggggcgca ccacgcggaa
     8161 cccgatcatg cctccgtccg tcaggtacca gatgctttta gggttttgag gatcctgacg
     8221 gttccaggcg ggggtgctgg cgcggcgcgc agcgcttctc aggctgtccg ggtcgtcttc
     8281 ccacgaaccg ccgcgaacga tgtgaggggc gccggggatg atgactaggg ggtctttcgt
     8341 gggcaagccg ctccgtttgc cgtaggcatc cggaacgtaa gagtccaaaa cccattccgc
     8401 cacatttccg tgcatgtccc ggagtcccca cttatttggt tttttagagc ctgttttctg
     8461 gtagcggtca ttggaattgt tccagtacca ggcgtactgg tccagagagg cttccccgtt
     8521 tccgtaagaa taggcgccgg cggaaccggc ccggcaggcg tattcccatt ccgcctccgt
     8581 gggcaggcgg tagtaatgcc cggtctgggc gctcagccat tcgcagaatt tgctggcggc
     8641 gtggtgggac atggagatgg cgggcaggcc gtgttcatag ccgtggccca tgcccaggtt
     8701 catggcggcg tagggggcgg taggctgcgt gacggagtcc cacagttcgt catccggctg
     8761 ttcctccaga agctggccgt ccttggcgcg cggacgcccg ttttccatga aggccgtata
     8821 aagttcccag gggatttccg tttccgagat ccagaaggga gagatgctca ctttatgctg
     8881 cggaccttcg tccggtttcc ggtgcggctc ttccgcggga ctccccatga ggaacgttcc
     8941 tcccggtatg gcgaccatgc gcagggaagc tcccgccacg ggaaccgttt ccgtgtagga
     9001 ttcaaaacgt gccggatttc cggaagcttc aatgagctga tggatttggc gcgttgtgcc
     9061 gaccagctcc cgggacggca ggtcatccgg aatgggaagg gcaggaccgg cagcgcggtc
     9121 cagttcccgt ttttgcagcg cggatcggct ttctccccac tggcgtgcgc tggtaatgcg
     9181 cgcggcatct tccgtggccg ggcgggggga attattttct tcccggcacc cggccagcag
     9241 ggcgcttcct cccagaaagc aggaaacaag gaaaatgccg cgcatggaat ggagaaaacc
     9301 ggaatgccct acatcgtcat gcctccgtcg caggcaatga cctgcccggt gatgaatcca
     9361 gcgccctcgg acgctaaaaa cagcgcgnnn nnnnnnntgc ccggtgatgt aacgggcatc
     9421 gtcagaggca aggaaggccg tcagggcggc gatatcttcc acggaacctt gttctttcaa
     9481 aggtattttc ttaacgattt cttccttgag attgtcggga atggcattgg acatttccgt
     9541 ggagatgaag ccgggagcaa tggcgttgca cgtgactttg cgcggagcga attcctgagc
     9601 cagtgatttg gtgaaaccga tgaggcccgc cttggaagcg gcgtaattgg cctgaccgat
     9661 gttgccgacg agaccgatga cggagctcat gttgatgatg cgcccggccg gggatttcat
     9721 gagaacgcgc tggagggcct tgaccgtatt gaacgcgctt ttcaggttgg tgtccagaac
     9781 ggcatcccag tcttcctctt tcatgcggag catcagggag tcccgcgtga tgccggcgtt
     9841 gttcaccagg atgtcaatct gcgggaagtc tttcaaaatg gcggccatgg tttcctgaac
     9901 ggccgcgtaa tccgctacgt cgcaggggta ggccttgcag gaatccggaa attccttgtt
     9961 gatttcttct gcggcccccc cgcagctgga tggactgcgg ctgatcagga tgaccctggc
    10021 tccttccgaa gcgaagcggc gcgcgacggc attgccgatt ccgcggcccg cgccggtaac
    10081 gatggctgtt ttacctgcta acttttgcat gctgcccaca gtaaacccgt ttttcacggt
    10141 tccggcaagc ttgaaatgga cttggccgcg caggaaccca tacggggaag gggcgtgccg
    10201 ggaatgaaaa aagagggggg ga
//
LOCUS       DAWEMX010000094         9836 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2884_length_9836_cov_10.932216, whole genome shotgun sequence.
ACCESSION   DAWEMX010000094 DAWEMX010000000
VERSION     DAWEMX010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2884_length_9836_cov_10.932216"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1323..1332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1850 a         2707 c         3096 g         2173 t
ORIGIN      
        1 gccagcaggt tttcttctgg aagggggagg gaatggcggg ggcggcggaa gtttgggtat
       61 tctgatgttg ttcgttgttc atgccggtgt agagagatgg ctggttgctg gcccggctca
      121 ggcagcggag gccttgtccg ttttcccgtc agctgcggga gaggcttcat ccggagtttc
      181 tccggccgta gccggaggct gctggccgtc tgcggagggc tgggtctgtt cttttttttc
      241 ctgctgcctg acgtggtttt cgaattcctg ttccgtaatg gcctggggcg tggggtaata
      301 atccccggca gggggcgcgg ccggaacttc ccattggatg cgcaccagcc tgttcctgcg
      361 ctggaccacc acgctggcct gagccccccg ctgggtggta acatattcta cgaggtccgg
      421 atagggattc tttcttccat tgatggaaat aagcacatct cccgtcctga ggccggcgtc
      481 ccatgcggga gaccactctg ccacgtaagc tttcatgggg ccgttcccgt tgctgtcctg
      541 gataaaggtg cctcccatca ggttgaagga ggcgggggcg ggcatccggt ggctggtgga
      601 gctgaaataa agggcgttcc ccgggccgtc cagccagaac tggcgtgcgg ccagagcgcc
      661 gtagcccagg atgatgtcga tgtcatcggg gatgcccatc acttccgggt tggcgaagct
      721 ttcacagagc aggatatttt ttaattctag tggaccgagc tggaaggagc ttacatgcat
      781 gcattcgtgg gcgtaaaagc cgcctgcggc ggggctgtag ccggaataga cgctgacgaa
      841 cgcatccggg taggcctgct taatggcatt ccagcgtttt ttggagatgt aaacggcgtg
      901 gggggctccc gtgtccagaa tgatgcgcct tccgtgcctg tccgcgattt gcggatagtc
      961 ggatccggga atcagggaaa gcttgttcca gctttttatt ctggagggaa gtttattgaa
     1021 aaaacggtgg gagcgtttgg gatagttgat gttccacaca tatttccgga tgcattccca
     1081 gcccagcaaa ccgtcatacg gggcctggtc tgccattact acgtcctgtt tccgggaaat
     1141 gggttccccg tcatccttca gggaaacagg cacgttggcc gtgcgcatgg cgcccctgcc
     1201 gcgcaggcgt gcgccgatgg attccactgc cggggagaaa agcagggatg tctggactgc
     1261 tcccgtgtct acgcccatca ttaattgcgt tccggagctc cacgccagcg ccacgggggt
     1321 gcnnnnnnnn nnggtgccgt ccaggggatt ggccgcgtca atgggcgggg agctgctgtc
     1381 ctggataggc cggtgcgata cgcaggaaac gcacagcatg gttgccagaa gcatgatgga
     1441 ggaacggaac ataaagtatt ccgttcataa caaacagacc ataggctggc aaggtttcct
     1501 tcctgcttac agggcttttt gacggcatca gaacgtaaaa accgctgcct gccggagggt
     1561 cgctcatgca gaagcatgat attcaaaatg cgggcgcgtg cgttttttga ggctctatat
     1621 catggacggg aattttgccg tctgccgcgt ccggcgttcc gttccagcga gggggcggct
     1681 gccttcattt tccccatgct gtccccaagg gagggctgaa gctggagcgc tcccaaaaat
     1741 cttgattttc acaaggagcc gtatagggta gcggtgagcc gctatgacca cccctacgtt
     1801 tgaagatgcc gtgagccgta tcgtacggaa ggatccccgt tttgcggaac gggcatattc
     1861 ttttctgaaa gatgcgctgg attttaccat gcagcgcgtt gaagaaaggg aaaacggctc
     1921 tcagaggcat gtcagcgggc aggaactgct ggaagggttc cgggattacg ccctcgccca
     1981 gttcgggccc atggcttcca ccgtgatgaa ggaatggggg cttaggaacg ggaagaatgt
     2041 gggggagatg gtttttttac tgattgagga agacgttttt tccaaacagc cggaggattc
     2101 cctggatgat ttcaagggct tcatgagctt ccgcaaggct tttgaagaac cttatgaatt
     2161 tcaggaccag cccagttgaa ccgcccgata ccgccatgaa caaggaaccc gatgtacgct
     2221 ggcccgctga atgggagcct caggatgctg tctggctgtc ctggccccac cgcagggatt
     2281 tatggcaagg ggggctggac gagttgcagc agacttatgg gagcgtggcc gctgccattg
     2341 ctccgcatgc ccttgtatgc gtgaatgccg cagctcccct tcatcccggc gtcaggcagg
     2401 cgatgctggc cgccggaatg agtgaggagc aattccgcct gttcaaccac ccgaccaacg
     2461 acgtatggtg ccgggaccac ggccccgttt tcgtccagga tgtgaaggac ggttctctga
     2521 tgctggcgga ttggcaattt aatgcgtggg gcggcaaatt tgccccgtgg gacctggaca
     2581 acggcgttcc cgccctgatt ggggcggcgc tggggcttcc cgtgcgcagt tcttccctga
     2641 ttctggaagg gggggcgatt gagggcaatg gggacggctt gctggtgacg acggagtccg
     2701 tgctgctgaa tcccaaccgc aatccggatt ggagccgggc catgattgag gaggaattga
     2761 agcgcatgct gggcgtcaga gccgttttct ggctcggttc cggcattgaa ggggatgata
     2821 cggacggcca tattgacgac atggtgcgtt ttgtgtgccg ggatgccgta gtctccatcg
     2881 tggaaacgga ttcttcctct ccccattacc gcgctctggc ggagaataat gaacgccttc
     2941 aggatttgag atgcgtggac ggttccgggg tggaggtgat tcccctgccg atgccggatc
     3001 ccctccatgc ggaggactgg cgcttggatc agctccctgc cagttacgcc aatttcctca
     3061 ttgttaatga ggccgtcatt gttcccgtat tcaaccagcc ccggaatgac gatcgcgcct
     3121 tgggcatttt gcgtgaatgt ttcagcggaa aacaggtaat aggggtggat gcccgcaagc
     3181 tggtgctgga aggcggcgcc atccactgca tcacccagca gcagccccgg ccggggaagg
     3241 agggactgtg agcggagggg aaggaataac ggtggatgtg attctgccga tggaaaaggc
     3301 cggcagcgag cgcgtgcgcc gcgctgccgt ggcgcggaag ctgggtattt ccccctcccg
     3361 tatcaaagat gtgcggctgg tgaaggagtc cattgactcc cggcagaaaa aaattctctt
     3421 ccagcttcgg ctgctggtcg gggtggatag cccgctgccg ccggaacgtc ttccctcccg
     3481 ggattatccg tccgtccgtc ccggctccct cgtggcgctg atcgttggat tcggaccggc
     3541 cgggatgttc gccgcgctcc gctgtttgga actgggtatg aagcctgtgg ttctggaacg
     3601 ggggaaggat gtttcctccc gccggtttga cctggctccc atcctgcgcc gcggcacggt
     3661 gatggaggat tccaattact gttttggaga gggaggggca ggcacgtttt ccgacgggaa
     3721 gctgtttacg cgcgcgacca agagaggccc cgtccgggag gtttacgaga tttttgtagc
     3781 ccacggagcg cccagggaaa ttttaacgga cgcccacccg catattggct ccaatctgtt
     3841 gcccaatgtc gtcaaagcca tcagggaatc catccttcgc gcgggcgggg aaattcattt
     3901 caatgcccgt gtggagcatc tgctccgttc tgcggacggc cgccgcatac gcggcgtggc
     3961 ctgcgcggat ggccgggaat ttgcggcgga ttccgttttg ctggctaccg ggcacagtgc
     4021 ccgggacgtg taccgcatga tgctggcgga ggggctggct ctggaacaga aatcctttgc
     4081 cgtgggtgtg cgcattgagc atccccaggc gtttgttgat gcgcgccagt accacctgaa
     4141 tccggagcag aggcgcccgg agcagcttcc ggcagcccgt tattccgtga cgaccaccat
     4201 ccaagaccgc ggcgtccatt ccttctgcat gtgtcccggc ggcttcatcg tgcccgccgc
     4261 cacggagaac gatgaggtag tggtcaacgg catgtctctg gcccggaggg attctccatt
     4321 tgccaatgcc ggttttgtag tcagcgtgca tccggaagat acggaaccgt tttgcaggga
     4381 acacggggtg ctggcgggcg tggcgtacca gaaagcgctg gagacggctt cctgccgtgc
     4441 cggaggaggc atgcagaaag ccccggccca gaaggtggag gattttctga aagggcgcgt
     4501 ttcttcttcc ctgctgccta ccagctatca tcccggcatc gtatcccatc cccttcatga
     4561 attgctcccg gaagggattg tctggcgcat gcgggagggg ctgcggcagt ttgaccgtaa
     4621 atggcgggga tttgccggag cggacgccca gctcattggc tgtgagacgc gcaccagctc
     4681 ccccgtgcgc attccaaggg accccgccac gctggagcat cccggtctgg agggcctgta
     4741 cccatgcggc gaaggggcgg gttacgccgg cggcattgtt tccgccgccc tggacggacg
     4801 ccgttgtgcg gaggccatgg cgcagcaggc gacccgctag gttttttcaa gcgtccggcc
     4861 gccggagcga tgcagctttt ctgcgtttct ggatgaagat acgcccctgt gagcccttgt
     4921 tatgccggaa ccggatgcca tcaagccggg aataggggca ggcttgtttt ttccgggaag
     4981 gaaagacagt ccgccgtggc ggcggccgtc aatgctgcgg gatgcggcgg aagggaagaa
     5041 tattgttgac cctgcgcaaa agattcctgt aacgtgattt catgtcaaat ctgttccggt
     5101 ggggaattct ccctgcgttt ttgatgactt tttccggcat gtcccaggat atagcggcgc
     5161 cctccatggc taatgtggat ttggaaatgg aggatgaaaa gaaaatagcg gcgtacaggg
     5221 aggccatggc caggcacttg cagctgggca ttatttggac gtgcattgtg gagttggagg
     5281 aaaaggataa tcgtgcggtg ctggtggcca cgctgaaata tattcaatct cccgaatggg
     5341 ctgggattct ggataaatgc ccttccgatt accgtgccat gcatctgaag atgattaggg
     5401 agtccgggaa attgctggaa cgggtggaaa gagagaagat gactgccgat gaaattcaga
     5461 acgcttacgg aaagtacgga ggccaataca tgaaaatggg ggggagcatt ctggaaaaat
     5521 atcggctgga gaattgttcc gtacagtttt ctttattcct gatgcgggaa acggaaggat
     5581 tggatgataa ggaaaggttg aaggctcttt accgtatcag gaaggatatc ctctccggaa
     5641 aactgaaggt tcccggggag ggcggtggga tgggcgaatc tgggttggag gaatgaccgg
     5701 gatgccgggc cgattccccg gaacggttcc ggaacccctg tgtttccttt ctcccgttgt
     5761 ccatttccac ccggggcctt cctttttttg caggttaagt cttgccaggg cagggggctt
     5821 gggggcataa tctgcaagtt tcacaaccgc acacacagcc atgaaagtca tcgtcatcta
     5881 cggcagcccg aatgataagc cctttatgga accggcccgc gagtattttg ctcaggagaa
     5941 tgttccttat gaagaaaccg ttctgtccgc ccaccgcgac cttccggagc tgatcaggtt
     6001 cctgggggat ttggaagcca gcggagaaaa agcggttatc ctggctgtgg ccggcctgtc
     6061 tgccgccctt cccggcgtgg tggtgatgag ctgttccctc ccggttatcg gcgtgccggt
     6121 ccccggaggc cccctgaacg gtattgacgc cctgctggcg attgcccagt gtcccggcgg
     6181 cgtgccctgc actacggttg gcttgcataa gaagaccccc gtcaatgccg ccatggccgc
     6241 ccaccgcatt ttaaaactgg cggggctgta acgtgaactt tttcccccat ccggcaaatg
     6301 actcctcctt cacaggaaat cgtattggac ggtccgggaa tttcccgtgc gcttgagcgc
     6361 atggcccacg gcattgtggc ccgtttcccc ggagaacctc tcgccatcgt cggcctgctc
     6421 agccaggggg atgtgattgc ccggcgtctg gtggacaagg taaaggagct gggagtggaa
     6481 gcccagtacg gctctattga tatttccctg taccgggatg acatcttcaa gctggaagcc
     6541 cggccttccc tccgttcctc caatttgccg ttttccacgg atgacatgag ggtcgtgctg
     6601 gtggacgacg tcctttatac ggggcgcacc atacgtgcag ccctgaacgc ccttttcgac
     6661 tacggccgtc cggcccgtat tgaactggcg tgcctgattg accgcggcgg gcgggaagtt
     6721 cccatccagc cggattatac ggggcacgtg ctggaccatg ccggagccaa ggtcatcgta
     6781 agcatgaagg aggctgacgg cgaggacagc gtggtcattc cctcccactg aaaattttcc
     6841 aatccattct ccgtttccca tgaatcatcc ccgcaaggat cttcttaata tctcctcatt
     6901 gactgacgag gaaattcaca cgctgctgga ttcggccggc ccgatgaagg agctttttac
     6961 caaaagcgtc aaaaaggtgc ccgccctgaa aggcaagtcc gttctgacgc ttttttatga
     7021 acccagcacc cgcacccgtt cctcttttga ggtagcggcg gaacgcctgt ctgcggacgt
     7081 gaccaatttc acggtgtcca cctcttccgt ggtgaaaggg gaatccgtac aggacacgat
     7141 cgccacgctc caggccatga aagtggatta tgtaatcgtg cgccattaca acagcggctt
     7201 gccgaacgtg attgcgcgcc atacctgcgc cagtgtggtg aacgccgggg acggagccca
     7261 cgcccatccc tcccaggcgc tgctggattc ctttaccatc cgggaggtat ttccggatgt
     7321 gaggggaaag cgcgttctga ttgtggggga catcctgcat tcccgcgtgg cccgttccac
     7381 ctccctgctg atgaagaggc tggggatgga tgtcgccttc ctggggccgg gttccctggt
     7441 gccgcgcacc gaacattccg gcattccccg tttttccgat ttcaatgagg cgtttgcctg
     7501 gaagccggac gtaatttacc tgctgcgcgt tcaaaaagag cggcaggacg cccctttctt
     7561 ccccagcgcc cgggagtaca acaagattta cggcgtcacg gaagaacgcc tgaagcgcat
     7621 ttccggtgaa ggcctgtaca tcatgcatcc ggggccggtc aaccgcggcg tggaaatctg
     7681 tgacctggcg atggactatg agcgctgcct gatcaaccgc caggtggaaa acggcatcgc
     7741 ctgccgcatg tccatccttt accatctcac tccgcaaacc cagcactgat tgttcatgaa
     7801 aacttccgta cttattcgca acgcccggct gacggacggt tccgccccgg cggaccttct
     7861 ggtttccggg ggggtgatta cagcgaaagc tcctgccgga acgcttttgg agagcgccgc
     7921 ggaaaaggcg ctggatgctt ccgggaagct tgttctgccc ggtctgttcg acatccatgc
     7981 ccatttgtgc cagcccggcc gggaggacaa ggaacgtgtg gctacggcta ccgccgccgc
     8041 cctgcatgga ggagtgaccg gactgatggc gatgccggat accgaacccg tgatggacaa
     8101 tgccgcccag attaccacgt tcatggaaat atgccggacg gatgctctgg tggaggttat
     8161 cccctccggg tgcctgacca agggggacgg cggggaagag caggcctcct acgactccct
     8221 gcgtgccaag ggcgtccgtt tcatcacgga cggggaccgt gctccggaca acatgctgtt
     8281 gctgtaccgc gccatgcagt acgccagcaa tctgaacctt actttcgccc tgcgcggaga
     8341 cgtgccgtcc ctgacggcca gggcggccat gcacccgggc actacttcct accgtctggg
     8401 gttgatgggt tccccctcct gtgcggagga aatagggctg gaaaccatca tccgcctttc
     8461 cgtggatacg ggcaactccc tgcatgtgca gaccgtctct acggccggca gcgtggatat
     8521 cctgcgccgc tgcaagcgga aaaccgccgg gttgagcgcg gaagcggcgc ttcaccatct
     8581 cctgttcacg catgaggacg tgggccggta cgatacctgc tacaagacgc tgccgcccct
     8641 gcgcgaccag tcggatgtgg atgccctgat tgccgccgtg aatgacggca ccattgactg
     8701 cattgtttcc gatcacacgc cctgcacgcc gttttccaaa cttcaggatt tcgttgtcgc
     8761 cccgcagggc atgattgcgc tggatacgtt cctgcccgct atttaccagt atctggtgga
     8821 acctggaaaa atgtcctggc agacggtggt gcgcgcctgc agcgatgctc cccgcaggct
     8881 gatggggctg gcccccgtct ccctggaaga aggggctccg gccaatctgg tggtgtttga
     8941 cccggaagga gaaacgcccg tcacggatga aacgctccgc agccgcgccc gcaacacccc
     9001 attcctgggc aagaccttga agggaaaagt ggatgccgtg gtgttcggag aacagctcca
     9061 ccgcttctga tgaaagcgtc acggagaaag gcaggcagct attaattatt aatctccgct
     9121 tgcagcccgt cgcccgcgtc agcacgtgtt atccggacaa attcggtgcg ccgcgccaga
     9181 gcggcttggt tccggatgcg cgggcgcggc tggtgtttga gccgccgttc cgccatccgg
     9241 atgccgttcg tggtctggaa gggttttccc atctgtggct ggtctgggtg ttcagtgaaa
     9301 acgtggataa ggggtggagc cctacggtcc ggcctccgcg gctggggggc aatgtgcgca
     9361 tgggggtgtt cgccacgcgt gcgccgttcc gccccaatcc catcgggttg tctgccgtca
     9421 ggctggaacg ggtggaattg catcctctgg acggcccggt attgcatctt tccggcgtgg
     9481 atttggtgga tggcaccccc attctggata tcaagcccta tgttcctctg gcagactgca
     9541 ttccggaggc gtcggaaggg tttacggctg tcccctatga acggctggat gtggaaattc
     9601 ccgaatcttt tggcggttcc atgtctccgg aagagaggag tgcgctggaa gatacgctgt
     9661 ccctggatcc ccgtccccag tatcaggatg atccggaacg ggtttacggc ctttcgttct
     9721 ctggctggga ggtgaaattc aaggtaaagg acaagcggct gcgcgtattg tccctggaaa
     9781 gtaggcgaag cggttccggc cttttttgat atgcgggcca ttccttaggt attttt
//
LOCUS       DAWEMX010000095         9813 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2890_length_9813_cov_11.084546, whole genome shotgun sequence.
ACCESSION   DAWEMX010000095 DAWEMX010000000
VERSION     DAWEMX010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2890_length_9813_cov_11.084546"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2846..2855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2158 a         2963 c         2506 g         2176 t
ORIGIN      
        1 ctcatcccaa ctcagcaaat ggctgctttc ttcatcaacc tcctcattcc tgacgtaagc
       61 cggcgtattc atttctgcat ccgtacattc ggttccttca tcagccatag cgcaacagcc
      121 ctgttttttt gtttaaaata aaaaacggaa aggcatggcc ctcccccttc gacggaagac
      181 ggccatgcct ttcggaacaa tttttacttc tcctgattga tatgtttccg catattgtca
      241 atccacggtt ccagtttttt ggcggcttga gactccggat cggcatcacg ggcaatttcc
      301 aggtattcca aagccttggg caaattacgt tccaaggtat acccatcact gatatatttg
      361 gcgatcttca ggcggatggg aggaagcgta tccttttttt ccaacatctg caatgcttcc
      421 tcccggccgg cgcgcgtttc ttctgcggag agcctccccg aatatttttg gcagaactgt
      481 tcccacacaa gccgctgctt ctccatggct gcggcttttt cagacttctt tgcaaaaccg
      541 tatttgtcct ccgggtccag agcggaaatt tctgcaatca accccttctg gaaaggctgc
      601 aaatcttccg gaagcacgtt caaggcctta accagttgct ccagtttttc ctttcctttc
      661 aatggctggg ccgccgctac ggcctctttc aacttcctcc tcgtttctcc ggcggcctcc
      721 agttttttca gatattcctc cggagttctg gtggcaccca ctattttagc gtagggagcg
      781 ccatttcctt ccatcaacag aacggatggg aacgaggaaa cgccataacg ccgcatcagc
      841 tcttcatgga attttaactg ttccggcggc attttcccgg cagccttggg gaaatccagt
      901 tccagcagca cgaattgatg atccgccgca tatttggcga acgcatccgt gtcaaaaata
      961 tttttgcgca ggtaaatgca tccgggacac cagtcggaac cggtaaaatc agccagcaca
     1021 ggatgtccgc cctttccggc cttctccagc gctttattcc aatcagtctc ccaggaggaa
     1081 gacgcggaac aaatcaaagc ggaggccaag gaaagaacac caattgcagg aaaaaatgaa
     1141 tttctcatct taaccgtact atagcaggat accggggctt gtcattcttt tttaagggct
     1201 ggccccctcg gaaccgcaag ccccgcgaaa tgccggagaa aagacgccta ttgctgtgag
     1261 cggcggcgga tatcctggct caggcgcatg gcgcagaagt ccggaccgca catggaacag
     1321 aaatgggctt ttttggcgtt ggcatgggga agcgtcaggt catggaagga acgggccttt
     1381 tgcggatcca gggaaaggtt gaactgatcc tcccagcgga attcaaacct ggcctgggcc
     1441 agagcattat ccctccattg cgcggatgga tgccccttcg ccaggtctgc ggcgtgggcc
     1501 gccagcttgt aggcaaccac gccttccctc acatcctccc ggtcaggaag ccccagatgc
     1561 tcttttctgg ttacatagca aagcatggcg cagccaagct ggccgataat cgcccctccg
     1621 atagctccgg taatatggtc gtaaccgggg gcgatgtccg ttaccagcgg ccccagcgtg
     1681 taaaagggcg cttccatgca ccattccagc tgcttgctca tgttctccct aataaggtgc
     1741 atgggaacgt ggccggggcc ttcattcatg acctgcacgc ccgctttcca cgcgcgcatg
     1801 gtcaaatccc cctgcacttc cagctccgcc agctgggcga agtcgttggc gtccgccacg
     1861 gaaccgggcc gcaggccatc cccgatggag acggcaatat cgtaagccgc cagaatggaa
     1921 caaatttcat cccaatggga ataaaggaaa ttttcctgtt cgtggatcat gctccactgc
     1981 gccatgatgg aaccgccgcg ggaaacgatg cccgtcatgc gccgcgccgt atggttcacg
     2041 aacctgagca gaagggcggc gtgcaccgtc acatagtcca cgccctggcg ggcctgttca
     2101 agcagggtat cgcgaaaaac ctcccaactc aggtcagcca cctttcctcc caccttttcc
     2161 agagcctggt aaatgggaac agtaccgatg gggacagggg agtttcttaa aatccattcc
     2221 ctcgtcgcgt ggatattctt cccggtggac aggtccataa cggtatccgc cccccagtga
     2281 atggcccagc gcagcttttc cacctcctcc tcaatgctgg atcccagcgc ggagttgcct
     2341 atgttggcgt tgatttttac caggaaattg cggccgatag ccatcggctc acattccgga
     2401 tgattgatgt tggcgggaat cagcgcacgg ccggaggcaa tttcatccct gacgaattcc
     2461 ggcgtaaagt aggcaggcat gcggcagccg ttccggcgtt ccggggcatg ctggtgatcc
     2521 aggcggtcgc gggccaccat gctggacggc ccaaatccct cctgcgcctc caattcggac
     2581 ggacgcggca tcatggtgag cgtttccagg gcttccgcag cttcgtccgg gcggctcttg
     2641 gcatttggat agcggtcgta tatcgccttg aaagcctgtt ccctgcccag attttcccgg
     2701 atggcgacaa attccatttc cggcgtaatg acgccctgtt gggcgtaata acgctgtgtc
     2761 acaggcgtct tgcccgcagc acgcagggtg cgctcgtgcc ccacatcctc tttcacatcc
     2821 ccgcgggccc gtatccaggc ggcacnnnnn nnnnnccctc ctccgcagtt ccctcataag
     2881 cagcgtcccc ccaggggccg gagcaatcgt acacgcgcac cgggtcatta gggtgggcgg
     2941 tgccgtcggg caacaaggta tcgcccaaaa tgatttccct catgggaacc cgaacatccg
     3001 ggtacagacg gcccggaaca taaatgcggc gggaaccggg ataagacaaa ttggaggtat
     3061 cgttcatggg atgaatgctg ttctgttccg gcaggggaac agcggaaacg aaacctcccc
     3121 gaaaaacggg acaagcccgt tcaatcgaac tccctgcggc ggaattattc gcatcaggtt
     3181 tgaagggttc tgcgggaaca gcatgccgtt cctgcaatct cagcctttat ttgggcaccc
     3241 ctgccgaact ggaaaaagaa taacacggac tttctcttca tccaagcgtt ttccagcccg
     3301 gcgcggtgaa accggagcgt caaaaagacg gactccgttc cctcccggca atcaacacgg
     3361 ggaagtttct ctaccctcca ggcggattca cgcgtttttc ctcctccatt tcagcagaaa
     3421 aacggagttg gcgatcacga cgacggagcc cgcattgtga accagcgcgc cagccacggg
     3481 attcaggatg ccgcccgccg ccaggatgat ggcgatgaag ttcagggcca tggagaatgt
     3541 cagattgcat ttgattgtcc ccatcatgcg ccttgacaga cggagcagat ggggcagttc
     3601 ccggatatcg tcattgacca ggacaatatc tgcggcatcc acggcaatgt cacttcctat
     3661 gccgcccatg gcaattcccg catgagaggt tttcaaggca agggcatcat tgattccgtc
     3721 cccaaccatg cacacacggt tccgctcttt ctggcacgcg tcaatccagt tgaatttgtc
     3781 ctccggcagg cattccgcgc aaaccaggtc aatccccagt tgtccggcga cacgcgcggc
     3841 ggcattccca tggtccccgg tcagcaatac gggaaccact ccggcatccc gtacttcccg
     3901 gatggtggct acggcattct cccgcaaggt atccgacagg gcgatcaacc ctgcggcctg
     3961 ccggtccatt cctatgaaaa tcacggagca cccctcctcc tgataccgtt ccgccgcacg
     4021 tctggcctca tcggacagct ccaccccgtt ttccctgaac aattccaggt tgccggccag
     4081 aagttccttt ccctgcgcca cagcccgtac tcccctgccg ggaatcatgc ggaattgttc
     4141 cggttgggga agtttctgca ctgaatcttt ccggtaacag cggacaatcg ccttgcccag
     4201 aggatgttca gaacgcagtt ccgcacaggc ggcatatctg taaagttcct gttccgggag
     4261 ctccggcagg aaactgcgca cggccgccac gcgcggagca ccgcaggtaa gcgtgcccgt
     4321 cttgtcgaaa gtgaggtggg acacgcagga caggcgttcc agggcatccc cttcccgcac
     4381 caggaagcca tgcctggtgg cgttcccgat agcggccatg accgccgtgg gagttgccag
     4441 cacaagcgcg cagggacaga ataccaccag aatggtaacg gcgcggatca gttcgcccgt
     4501 ggccatccag gccccccctg cggaaaccag ggcgatggca acgatccagg tggcccagcg
     4561 gtctgccagc ctcactattt tagccttccc cgcatctgcg gattggacga ggcgcaccat
     4621 gcgctggatg gaactgtcct tcccggtcct ggccgccttc atgtcaaagg ttccgaactg
     4681 gttgaccgtc ccgctggaaa cctcatcccc ggtttccttg tctatcggaa gaggttcccc
     4741 cgtcattacg gactggttga cggaagtgtg cccggaaaga ataacgccgt ccgccggaat
     4801 cgtctctccg ggcaatacgc ggagaatatc cccgatgcga acctcttccg ccggaataac
     4861 ctcctccctg gttccttcca ctttccgggc ggtcgccggg gtcaaacgga ccagcctttc
     4921 aatacccttc cgggcccggg ctacggtcag ctcctcaagg agttccccaa gctgcatgat
     4981 aaatgcaatt tccgccgcag cgaagtcctc cccaatgatg acggaggcaa ccagagcgac
     5041 ggaaacaaga acatccgcct taatatccca ggccgtcgcc aggccaagga cggcttccag
     5101 cacaatggga acgccgcaca gcacaatggc tatccatgaa atttcaaagg gcaaaggttc
     5161 catgccaagc atgctcatca acagagctaa tccggaaacg gacaacagtg cgactttccg
     5221 ccttgtccct ccccattcca gcaatttttc caatcggtcc agcatatgat gatttttctt
     5281 ttcataccca tacgggtacg ggcatttata tacctatagg ggtataggtt tttcaagaaa
     5341 aaaatgagcc ccccctcccg aacctgccgg aaaaaggcat taaccccttg tcccattcaa
     5401 atcatgttgg caaaccgctc aaccgccttg gtgaagcgcg cgatcgtctc ttccgggtct
     5461 ccgtgttcaa gtccgtcccg gacacaatgg ctgatatgcc cctccagtac gacctgcccc
     5521 accttatgca aggcggactt ggcggcattg agctgtgcaa gaacatcttc acagggaacg
     5581 tcttcgtcaa tcatcctgtc gattgcctgc acctgtccga taatcttctt gaggcggcga
     5641 tgcaaatttt cggaattcat gcattgtttc atggcggcgt ttcctgatgc tgctgagggg
     5701 tactcgtatt atcaagttat caggcagaca ggaaatgctg tcaagaacag agtcctcccc
     5761 cggcaggcgg ctccgtgcat cttccaacgg aaacaccgga cggtttttca ttatttcccc
     5821 ctctttccct tttgccgggg aactttctcc attccttgct cttctttccg cgcggagtgt
     5881 ggttgggagc cagggatatc cgagagaggc caaatccttc ctcacaaaat ccggcaccac
     5941 cccggctatt cccctgccgc gtttcctttc ctctacaaaa agagcacata aattgggagt
     6001 taagtccttc cggtcataaa aatcgttttc aattactccg gttcccgcaa tgatattccg
     6061 ctgcccgtcc acaataacgt accattgagg aatccctgtt ttccgctcca tgcattcctt
     6121 catgctttct tcataaactt ctgagggaat accccatttg gaactgaacc aggaagaagc
     6181 cgagaaaaga aattcggaat gattccttaa acaaattaat tagccgatat tcctttcttc
     6241 cattcttccc tgccctgaca agccccttcc ggcccctgtc aaatcggcgg gcatggaggg
     6301 aatccatcgt gcaccgggtt ctctcctgca aaggcgcgaa tatttcccgg gctttggaaa
     6361 gagtaaaaca ttttacttcc attccctgcg gaaagaaaag aaagacccgt tttcatctag
     6421 tcagccgtgt aaaaatggga tagacctttt tccggaatgc gccctgccgg ctgcaacctt
     6481 tcaccggaga cggggtttat aaaagggaat ttcatggatg aagcacaaga catgccgcct
     6541 gcggaaccag acgaagatgc caagctgatg ctgagggtca ggaatggcga cgcttccgcc
     6601 atggaaatgc tggtccgcaa acaccagaat tccgtatatg cgacggtggc ccgtatgctg
     6661 aacaacggtc cggagacaga ggacatcgcc cagcaggtat tcatccgcat ctggaaggga
     6721 gccgggaatt atgaaccttc cgcacggttt accacctgga tgttcaccat cctgcgcaat
     6781 ctggtgttca atgaagtgcg ccgccagaag cgcaagccca ccacctccgc agacgccatg
     6841 gaggaggaag gaggcatggc cgtgtttctg gaaccttccc agaccccgga cgaagcactg
     6901 gaacatacgg aactccagca cgccgtggac gcggcaatcg ccgctctgcc ggaaaaggcg
     6961 cggctggccg tccaactgcg ccgtttcgaa aacatgccct atgaggaaat agcccgggcg
     7021 ctggatatga cggttcccgc taccaaaagc ctgctgttcc gcgccagaaa catgctgaag
     7081 gaggctcttg cttccttttt atcctgacaa tggcgtcctc aaaacaagtt aagaacacac
     7141 cgccggcttt cccgccaaag ggtggggagc cggttcctgg tacagtttcc cgtctttagc
     7201 ggcaaataac ccgtagcgca gcggccaagt cctttcacaa aaacacccgg aaacaaaaaa
     7261 aaacgggccg ttctccaacg aaaacggccc gttgaacatg ttaccggaaa acgaaatcag
     7321 cgcgcatttt ttgcgttaag aacatcctta tagggaattt tcaggaagtt cagcgccccc
     7381 tgggattcgt acagcacgcc cacatggttc ttatctaccg gcgccaggca ggaatatccc
     7441 cagcctttgc gggcatcata aagaagccat ttgtcttccg gccatgaccc ggcatcattc
     7501 gtagaagctt tcaacgtcat gtgggaacgt ccggatgtgg tattgggatt ggaaaacaga
     7561 accactctgc ccgcgcccgg aacgccgtcc acggcaagca ggctgccctg gcagaccggt
     7621 tccttcagct gggcagtgcg gttggtgggg tgtttttccc acgtttggcc caggtctttc
     7681 gtaacgccca cgatgcggga accgccccag ttgcatcggg cgttgatcat gacggagccg
     7741 tcttccagct caatcacctg ggcttccgtc gtctgctggt tgacgcccgt gccgcagtgc
     7801 caggtttttc cccggtcttt ggaataaaca atggtggacc acggcacccc tttgccgtcc
     7861 cagtactggg cggcgaagac cagcgtgccg tctttcatgc aaatgccgtt gccggggcca
     7921 ttaaataaaa tgcgccaatc cttgttcttg gtttgttccg tgatattgat cggactggac
     7981 caggtcaggc catcgtcatc gctgtaggcc agcaccagct gtccgcaggc ctccggagaa
     8041 ttgtctccgg acttgctgcc ccagatggaa tgcctgtggc tccatatggc ggccacccag
     8101 atgcgccccg tcttttcatc caccagaatg gccggatccc ctacgcccct ggtagccccc
     8161 agagaggggt caatcttgga atcatcaatg gcgattttga catcagacca ggtgcggcct
     8221 ccgtccgtag agcggcttac gcccacatca atgttggccg gaaggtctcc ggaatggttg
     8281 taccggatat cgtacacggc cagcagggtc cccttcctgg aacgggccag accgggaata
     8341 cggtagaatt ttgatttgaa atccccatgc ctgactacgg ccacgccgat gcgctgcgcc
     8401 acgggagccg cattggcaac cctcaccggt ttattgcccg ccacaacgct ggccggacgc
     8461 accaccaccc tgccgtccag ggaggctcct tctttcatcg tcacgcaagc ccacaggtaa
     8521 ttgtctccgg gctccagctc cattttcccc ttcacagaaa tctggggtct ggcggaaccg
     8581 gcaagcttta ctgtaccaag aacctgggaa gagtctttcg ggaacggctc ttccccatga
     8641 tggatgatag gggcctcttc cccccgtatt acagccacgg actcaatgtc ggaaagcttc
     8701 gtcgtacccg taaaatccag attgacagct gtcaggaact gaggattaag gcagccgtcc
     8761 gtccggacat tcagagaaag aacggggctg cacgggatac gcaccatcac cgggtaagcg
     8821 gaagaggcca tctccacctg cggattcact ttcatgggaa tacagggaac aagaaacagg
     8881 tcgtccagct tcattccctt gttttccgga gaagtaagcc tgaacaccag ggaacgcgtt
     8941 ccggcaggaa cactggcttc tatcctggta tgaaacccgc ccgtcctgat atccttgccg
     9001 tcataaattt tcttttctcc attcggccct atggccacaa tggaaaattc aaaaggggcc
     9061 tgcccggtcc atcgctccgc ccaggcagaa agccggactt ccctggaagg cgtatccttc
     9121 aagtccagtt tcaattcagc atcctgtccg ccgaacatcc gcagggaggc ttctccggat
     9181 tttccccctc ctccgatttc cgccacaccg ttccggcagg acataaggcc gtatttggtg
     9241 gaaagcgttg taaatttgcc acgcctggcc tgctcaaagc tttccttgcc gagaacggag
     9301 ggaatagaca ggcccagtgc gcacaacagg ccaagcccca ttaatttatt caaagataaa
     9361 cgcatcgtat ttatggtgtg tttcgcggga tatatacgcc gccccccgcg gcaatcttgc
     9421 ccggagcagc gttatttgcc ggtccgtttc ctaacacacg gctttccgtt gcgtccagcc
     9481 ccgcttgaac ggcctgagga aagaccagtc cttacattcc cttttttcct ccggaaagag
     9541 ccgccggctc cacggaaatc acgggacgtt ccgggaaaga actggaattc cggtgcatat
     9601 aataatgagc ccagtgaaga accaccttgt cccccggttt aagggcggat atcttcttca
     9661 aaatatccgc actctgcagg atgggcttgt gcgccggatt aacgtcaacc atcagctgat
     9721 cctgcttttc atcgccgtac ttgcctggtt tccggtaatc caggtacttg acgatccgga
     9781 aaacggcgaa atccctggca tggtcgcact tgt
//
LOCUS       DAWEMX010000096         9614 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2966_length_9614_cov_13.189560, whole genome shotgun sequence.
ACCESSION   DAWEMX010000096 DAWEMX010000000
VERSION     DAWEMX010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2966_length_9614_cov_13.189560"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1789 a         2842 c         2918 g         2065 t
ORIGIN      
        1 tcccggcagc gcctggggga ttgcccatga gttcggccac gtcaaccagg tgcgccccgc
       61 catgaaatgg gtctccaccg gagaagtgac caataacatt tactccgctt acgtgaatta
      121 catgctgaat ccctccagca tgcgcctgga gcatgaacgc atcaacggcg gggacggcaa
      181 catgatcggc ggccgcttca acgcctatct gaacaacggc attttgaagg gggaaaactg
      241 gcttgtccag agcgggccgg acaagcgttc cggtggggat aaccgtccga tggtgcacga
      301 ccattttgtc aagctggccc ccctgtggca gcttgagctg tatttcaagg tagccgggaa
      361 gggtaatccc gacttttatc cggatatctt ttataaggcc atcaagatgg ataccagggg
      421 caagaaggac ggcgagctcc agcttgcgtt catgaagaac gcctgcgacg ccgccagaca
      481 ggacctgacg gattttttcc ggaaaacggg catgctcaaa cccattgacc aggaattgga
      541 cgactacacc tgcgcccgca tgaccatcac ggaggccgac tgcaagaacc tgattgccta
      601 cgccaggaag tacaaaaagc cggaaagccc cgtcatttac tatatttccg tcaacagcgc
      661 ggaagcatat aagaaccgcc tgcctgtccg cggcgtttac aaccagggcg ttacggagca
      721 gggcaacagg cgcattgttt cccatgacgt gtggaaaaac gccgtcgtct ttgaaacata
      781 caaagacagg gaaatggtcc gcatcaccat ggtggggacg gattccaggg ataattcctc
      841 caccacggtt ccctatccgg aaggttctac gcggattgaa gccgtttcct gggacggcag
      901 gcggacgctg gtgtacggca aacgccctgc caaatgaaag cttaagaggt tagcgtgttc
      961 gggcgccgcc gttctccggg acggcggcgc tttttcatgg caggggggat ggaaagtcgc
     1021 agggagattg tttctcctga tggtctttcc gccatgccgt gttttcggac cgctgacagc
     1081 cggaaggctg ttacttttga acaggggcgg gggcaaggat gtataacatg ggatggataa
     1141 ggcagggact tccggaggca tcaggggaaa gatgctgctg tttgtcgcct ttttaatcgt
     1201 gtgcttcaat ttgcgtaccg ggtttgattc tcccgatccg ctgctgggaa cgattgaaag
     1261 ggatatgggg ctttccctgg aaaattcagg gctgttcgcc ctgttgcccg tctttgtgct
     1321 gggggtggcg gctcccattt ctccacgcgt ggcacgctgg atgacgccgt ggaaaatcat
     1381 tttctggttc cagcttctgg cggtggccgg aattttctgg cgcagctggg acggcgtagc
     1441 ggggttatac ggcggcatgg ttttgatggg gctgggcatg ggaattgccg gggcggccat
     1501 tcccggactg attaagcacc agtttcctga ccatgcttcc gccatgatgg gggtttacag
     1561 cgccatgatc ggcgtgggca gcgctgcggc ttccggattg tccgttccca tttccaacat
     1621 gctgggaggc tggcgtttcg gcctgggggt ctggatcatc cccattctgc tgggaatgct
     1681 ggtatggggc gcttattttc tcaggcaccc ggccggcgtg tttcaaagcg atccggcggc
     1741 ttccggtcac aacctgctgc gcagcggcaa agcatggcag gttaccgttt tttacttgag
     1801 ccgcgttggg gcggcctatt tcttttatac ttggattccc atctttctga ggcagcgggg
     1861 catgagctat gaggatgccg ggttcatcct ttccgtggcc atgttcgccc agctgcccgc
     1921 cacgctttcc gcgcacgtgc tggaaaaggc tacgggcggc cgggggctcc tcatcgtgat
     1981 ggccatggca ttggcggccc tttcctgctg gggcatcctg tatctcccgc tggactgggc
     2041 tctctggatg gctattctgt tcggcctggc gacgggaacc gtattcagcc gcggaatggc
     2101 gctgatggtg gaacgggccc ggaccccttc cgaatcaatc aggctttccg gcatgtccca
     2161 gggaatcggc tttaccatgg gcgccctgtt gagcctgctg tttacttcct tcctgcatca
     2221 gggcggctcc tttttgccgt tctgcctggt ttataccttt ttctgcgtgc tcggcatggt
     2281 ttccggccgc atgtccgccc ggccgggata cgtgtaattt gctggggaga tttaatggtt
     2341 aaggattagt gactaggaag agaggttcat tgttcttttc ccccctttcc ggggtccgcc
     2401 attttctttt gagtgacctc cccgcggccc gaagggcttg acgctggcgg gggggacggg
     2461 taagctcttg cccgtcaaat tttatgaagg aagagattgt acgcatccaa cgggacgccc
     2521 tggcacgcat tgcccaggcg tctgacaggc gcggcgtgga agacgcgcgc gtggccatcc
     2581 tcggcaaaaa aggggaactg accctggccc agacgggcat gaaggatgtt cccagagagg
     2641 aaaagcccgc cgtcggccag ttgcttaacg aggcccgcaa ggccattacg gaagctctgg
     2701 acgccaagct ggaggaagtg caggcccagg cggacaaggc cgccgtggcc ggtgtggacc
     2761 tgacgcttcc ggcccgctcc ctgccgcctg gcggcctgca tcccctcacc atcgtgaggg
     2821 atgaagccgt ccgcattctg cgccacatgg gctttgccct ggcggacggc ccggagattg
     2881 aggacgagtt ccactgcttt gacgcgctga acacgccgga agatcacccg gcccgcaatg
     2941 agaaggatac attttacttt gattccggca agcttctgcg tacgcacacg tcttccgtgc
     3001 agatccgctc catggaaaag cagctgccgc ccgtgcgtgt catcgctccc ggttccgcct
     3061 accgccgcga cgaaattgac gccacgcacc tttccgcctt caatcagctt gaaggcctgt
     3121 atgtggatac ggacgtttcc gtgggcgacc tgaaaggaac gctggaatat tttctgcgcg
     3181 cccttttcgg ttccggaacg gaggtgcgct tccgccccca tttcttcccg ttcacggaac
     3241 ccagctttga aattgacgtc aagctgaagg tggacggcca ggccccccgc tgggtggaga
     3301 ttgccggctg cggcatggtg gatcccaatg tttttgaagc cgtggaccgc gaactgggcc
     3361 tggaccccgg agcgcaggcc cgctacacgg ggctgaccgg ctttgccttc ggcatcggcc
     3421 tggaccgcct ggcgatgatc cgctggggca tcagggacat tcgcgccctg attgagaatg
     3481 atgtgcgctt ccttgcccaa ttccaataat ttttacctgt tttcccgtta tgaagatttc
     3541 ccttaactgg ctttcccagt acattgacct gtccggcctg agcgtggatg aaatgtctga
     3601 catgctgact tttgccggca ttgaggtgga agacatccgc cagcagggcg tggattcccc
     3661 atacgtggtg gtggcccgcg tggctgccgc ggaacagcat ccccaggcgg accggctgaa
     3721 agtctgccag gtggacgtgg gcgacggcac gctgcaccag attgtgtgcg gcgctcagaa
     3781 ctacaaggtg ggggacaagg tcccctgcgc cctgccggga gcggtgctgc ccggcaacgt
     3841 tgaaatcaag gtgggcaagc tgcgcggcgt ggagtcccgc ggcatgctct gttccgcctc
     3901 cgagctgggg cttcccgaca aggagcacgg tctctggatt ctccctcagg aactggaacc
     3961 cggtacgccg atttcccagg ttgtcaaggc ggataccctg gtggaagtgg aagtaacgcc
     4021 caaccggccg gatttgcttt cccacaacgg catggcgtat gaactggccg ctatctccgg
     4081 ccgggggtac aggcccgttt ccattgacga cgccggagtg gcgctggaac ctgccggaga
     4141 cttcgtgcgg ctggaccagc cggagctgaa cccgtactac accgccgtaa aaatcagcgg
     4201 cgtgaaagtg cacgaaagcc cggaatggct gaaggaacgc ctggtagccg tcggcctgcg
     4261 ccccatcaac aacatcgtgg atattaccaa cttcgtcctg catgaactgg gaacgcccct
     4321 ccatgccttt gacgcggcga aagtgcaggg cggcatcgtc acccgcaccg cttatgaagg
     4381 ggaaaccatc aaggccctgg acgggcagga atacacgctt aactgcacgg atctggttgt
     4441 tgcggaccag tccggcaagg cgctcgccat cggcggcgtg atgggtgggg agaaaagcgg
     4501 cgtgacggac gccactacgg atatcattct ggagtccgcc tggttcaagc cctcctccgt
     4561 ccgcgccacg tcccgcaggc tggccctgtc ctccgactct tcctaccgct ttgaacgcgg
     4621 cacttccgcc tggaatgtcc tgcgcggttc cgtgcgcgcc gtggaactga ttcttcagct
     4681 ggcgggcgga acggcctccc caacgtatgt ggccggttct cccgttccta atccggctca
     4741 tgcttccatg ccttcctgcg gaggggcgga cggccccgtt tccgtgttcg cttccctgaa
     4801 gcaggggaag ggcgccacgg tgactaatga actgggcttt gtacaacttc cctggaaagc
     4861 gctggatcag atttccggcg gttccatttc tcatgaggaa gggactcgca tcctgacggc
     4921 gctgggcctg aagcaggttc cggattcacc ggaatgctgg ctgatccccc cccaccgcct
     4981 ggatctcacg cgcccgtgcg accttctgga ggaaatcgtg cgcgttttcg gcctggacgg
     5041 cataccgtcc cgcttctccg gccccttcgt ggcggaatcc ccggtggatg cggcctataa
     5101 cttccagatg gagctgcgcc gcaagctggc ggccctggga ttttatgaaa cccagaccat
     5161 caagctcatc gcctccgaat ccgcggacgg caccatcgcc caggtgaagg acgctttgcc
     5221 tctgcgcccc cttcaggacg gtgacctcat ccgcgtatcc ctgccgctca gcgaggatca
     5281 ctccgtgctg cgccccgccc atacgcccgg cctgattgcc gccgccgtgc gcaacagcaa
     5341 ccagggcgtt tccggcctgc gcttctttga acttggacgc gtcttccgca atacgggcgg
     5401 cggcaagggc agggacattg aaacggatac cctgggcatc ctggtttccg gggaccgcac
     5461 ggcgcgctcc tgggcggatc ccaggccgga gcaggcctcc tttgaggatc tgctggccgt
     5521 gatggcggct ctggcgccgg gccaccgttt tacgctcacg cctgccagac cgcgcgaaca
     5581 ggccgccctg ggggcggacg tacagctgga cgggaaagcc tgcggctatt tcgcccgcct
     5641 gtccctggcg cgttgccggg agctgggcct gggtcagccc gtgtacgtgg cggaactgga
     5701 tctccgtaaa atgcaggaaa tcctgaccgc gcccgtgaag gctgcggaat tgccccagtt
     5761 cccaggttct tcccgcgatg cggccatgga actgcccctc tccacgccca atgcggacat
     5821 tgtcaaggcc gtggaatccg cacgggaaaa actgcttgtc agttatttct gctttgacgt
     5881 gttcacggac ccctccggtg aaaagctgcc ggcggaccgc aaatccattg cctatacctt
     5941 cctgtacagg gatccggcca agaccctcac ggccgcggaa gtggatgcag cgcaccggcg
     6001 cgtgctgaaa gccctgacgg acaaagtgaa gggcctttcc ttcaggtaat ccccgttaac
     6061 gggccgcgcg tttcatgggc tggagcgcgc ggcctgcttt tcattttcca tgcatttgac
     6121 tttcctgcgc cgcgcgtgcc cggccgccgc ccgcatcctg atgacggcgg cgtggttctg
     6181 tttctccgta tgggcggcgg gcgtggtttt ttacaacgta tgggggggag ccgtgctggt
     6241 gtggctgtac gtggcggcca tggcatgcgc cttcgccctc cgcggaaaac ggcccgtatt
     6301 atggcgcgct tcctggggag tgccggccct gctgctggcg tactacctgt tcataccggc
     6361 gacgaacgat aaggaatggc agccgtcctg gagtcgcctg ccctccgtgg aaatcaacgg
     6421 aaatgaaatc gtggtgaagg acgtgcgcag ttttatctac cggacggagc gggacttcga
     6481 cgcgcgctat gtgacgcgcc gctttgacct ggacaagctg gctacgctgg attttgccgt
     6541 gagccactgg gatgggatgg aattcgtggc ccataccatg ttgtccttcg gttttgagga
     6601 tgggaagcat ctggccctgt ccgtggaaac gcgcctgccg gagggggtgg agcagggaac
     6661 tgtccctggc ctttacaagc aattcaacgt gatttacatc ctggcggacg aagaagacct
     6721 gttcgccctg cgcaccaatt accggaaaga ggacatgtac ctgtaccgca tcaacatcgg
     6781 cagggaaagc ctgaaaaagg cctttctggg gtttgcggaa aaaatcaaca gcctccatga
     6841 acgcccgcgc tattatcata ccgtcaccgc caactgcacg acggaactgg tagatacatt
     6901 caaaagttat ctgggcgtgc gccgctggca gtggactccc gtttttaacg gcatgtgtga
     6961 tcagaatgcc tatgaccgcg gggaacttct ccaccttccc ggagaaggtt tccgggagct
     7021 gaaaaagcgc tctttcctgg ggcatggagg gaacggtgag gattggccag ccctccgccg
     7081 ccgctgggag gagggctgga ggacgttcgc gtccgctccg gtcaaagagt agcgaactgg
     7141 aagtgcatcc agtcgtagtc gcgggagcgt cccagggata cggccccctc cgcttccacg
     7201 aatcgccaga aggcggcgta ctccggtccg gacaataccg ctttcggcgc ggggatgttc
     7261 aggccgtttc tgtccgggtc catgtccacg gcgattcccc aggcgtgcat ggatgtggcc
     7321 tttccgccga tgatggagcg gttgttgaag cagccgccgt acaggtccag ccccagttcc
     7381 ctgattttct ccatgccgta atggtccaga gtagcctgga agatgcggac gagcggttcc
     7441 gccaccagct tgtggcagcg catggtggaa acggtcgtgt ttgtttccca cgccagccgc
     7501 atgatataag gcagcttgat gctggtcatg ttgctttcat cacccgcacg gccgaagacg
     7561 gatttcccgg agcgcacttc ctcctggctg ggccaggatt gttccggttg gcggatgccc
     7621 agcctgtcca gcagggccag cgccgtcttg gggccgggga tgccgtccgc atccacgccc
     7681 aggtgctgtt gaatcaattt ccagtatttt ttcataggga gccacgctag aaactccctc
     7741 cggaaagttc cacatttttc cttccagcgt cattcctgcc tttagcaaaa aaaggccgtt
     7801 tgtcctttta tgttgcggaa aagattcatt ctgctacact tttgtttacc ttttatttta
     7861 tgaattcaga accatccaac gttgtcaaat tttacaatgg ggaacaaatt cccctggaac
     7921 tgcataaagt ccgcgtggtg cagaagctgc atcttgtccc cgtggaacgc cgcctggaag
     7981 ccgcacggga agccgggttc aacaccttcc agctcagcac gaacgatgtc tatctggaca
     8041 tgctgacgga ttccggtgtc aacgccatga gcgacaacca gatcgccgcc atgttccggg
     8101 cggatgacgc ctatgccggt tcccagagct ttgaccgcct gaagcaggct gtccgggatg
     8161 tcttcggcaa ggaatacctt ctgcccgccc accaggggcg cgcctgtgaa aacatcattg
     8221 cccgcacctt cgtgaagccc ggggacgtgg ttccgatgaa ctaccatttc accaccacgc
     8281 acgcccatat tgacctgaac ggcggcaaga ttgaggaact ggtggctgat gaagccgtca
     8341 atccggtcag caccaatccc ttcaagggca atctggaccc cggcaagctg cgggactgca
     8401 ttgcccgtca cggggcgggc aaaatcccct tcgtgcgcat ggaagcctca accaacctga
     8461 ttggcggcca gcccttctcc attgccaaca tgcgggaaat ccgcggcatt tgcgatgaat
     8521 tcggcatcat gctggtgctg gacgcctccc tgatcgggga aaacgcctac ttcatcaaga
     8581 tgcgtgaaga cgagttcagg gatgcgtcct gcgcggacat cctgaagacc atgtgcggac
     8641 tggcggatct ggtgtatttt tccgcccgca aggtttcctc ctcccgcggc ggcggcatct
     8701 gcacgaacga ccgggccatt gccaagaaaa tggagcatct cgttcccctc tttgaaggct
     8761 ttttgactta tggcggcatc tccgtgcggg aaattgaggc catggccgta ggcctgtatg
     8821 aaaccacgga tttgactgtg atttcccaga gcccctcctt cattgaatat ttcatcggcc
     8881 agatggtgga catgggcatt ccctgcgtaa cccccgctgg cggtctgggc gcccatattg
     8941 acgcagggcg tttcctgccc catatcccgc aggaggacta tcccgccggg gctctggcgg
     9001 cggctttctt catcgcctcc ggcgtgcgcg gcatggaacg cggcacgctt tccagcgtcc
     9061 gcgacgaaaa cggcaaggac attctggcgg atgtggaact gctccgcctg gccttcccgc
     9121 gccgtgtctt cacattatcc caggtaaaat acgtggcgga ccgcatgaag tggctctatg
     9181 acaaccgcga tttgattggc ggcctggaat ttgtggagga accgcccgtc ctgcgcttct
     9241 tcatgggcaa gctccgcgcc aagagcgact ggcctgaaaa actggcggcc aaataccgcc
     9301 gggacttcgg ggaaagcctg taaaggctgg ttctcccgtt caattccgga ttggcaaggg
     9361 ctggaccgtc aggttcagcc cttgttgcgt tggggggggg cgtgtctttg cggacatcac
     9421 ccttcaatgt ttccgccgga aagcggccgg aatcagaagt taagcagacc ctgtttccag
     9481 catacccagc cgattgccgt tgcgatgagc aggatgatga ggaaagccgt gcctgcggaa
     9541 gattttttcc tggcctgggg aagagccttc ctcgatactc cgccggcctg cagggtgcct
     9601 gccgccgcca tggc
//
LOCUS       DAWEMX010000097         9609 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2968_length_9609_cov_11.036215, whole genome shotgun sequence.
ACCESSION   DAWEMX010000097 DAWEMX010000000
VERSION     DAWEMX010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_9609_cov_11.036215"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4373..4382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6674..6683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8135..8180
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2288 a         2851 c         2521 g         1883 t
ORIGIN      
        1 gttgcgctga tgcagggcga tggccggaag aatattctct acggagtccg catccggctt
       61 gtccatgcgg tccatgaact ggcgcacgta cggggaaaag gtttccatat aacgccgctg
      121 gccttccgca tacagcgtgt gcatggccag acttgttttc cccgatccgg aaggacccgt
      181 caccaccgtc agccgcccca ggggaatgtc cacatccaca ttctgcaaat tatgctcacg
      241 ggcgccgcgg atgcggatgc gcccatcctc acaatgactc atggagaaag aatatcatca
      301 atttggaatc attccaagag aagcgcctgt catgaatccg ggtttccaga ggagtccggc
      361 caggtccaac ctgcctatta cgtcatcatc agcacaaatg gagggccgtg cttgcatttc
      421 tcccggtagc cgctagtatt tttcgattct tagaccgctg aaagcgactc tacctcgtca
      481 attttcaaca agacaaagat gattaagtgg attctaacca agattgtcgg caccaaaaac
      541 cagcgtgaag tgcgccgtct gcgccccatt gtggaacaaa tcgtctccat tgaagaatca
      601 tggaacggca agggacagga attcctgctt gagaaaacga gggaatggca agggtacctc
      661 catcgcttcc tccccatgga tctgcccccc gtgcgcattg tggaagccgc acccagggaa
      721 gagctggagg aaatagccgc caaactgaac gcccgttttg aatccctgaa aagcgaattc
      781 gcttccctcc ccgcggtgga agccactccc gcctccattg aagaagggaa agccgcctgg
      841 aacaacatta cgccccagtt tgacaagctg cgcgaacgct acctgaacca gatactgccg
      901 gaggccttcg ccgccgtcaa acacggcgcc cgcctgctct gcggggaaga acgcgatatc
      961 tgcggtcaaa aacaggtttg ggacatggtc cactttgacg tgcagctgct gggcggcatt
     1021 gccctgcacc gcggctacat tgcggaaatg gccacggggg aagggaaaac gcttgtcgcc
     1081 accctccccg tttacctgaa tgccctgacc ggcatgggtg tgcatgtggt gaccgtgaac
     1141 gattatctgg cgcgccgcga ctcggaatgg atgggcatgc tcttccagtt cctggggctg
     1201 actgtcggtt gcatccagag catgatgccc tcccagctcc gccgggagca atacgcctgc
     1261 gacatcactt acggcaccaa cgccgaattc ggcttcgact acctgcgcga caacggcatg
     1321 gcaacctcca agtccgaaca ggtgcagcgc gggcactact tctccattgt ggacgaagtg
     1381 gactccatcc ttattgacga agcccgcacc cccctcatca tttccggccc tgcggtcgtc
     1441 acccgggaac agcagtacga taccctgcgc cccgccattg agcgcgtggt gaaggcccag
     1501 acggaccttt gcaacgaact gatggcccag gccctgaaag cccaggaaga aggccgcacg
     1561 gaagaagtgg gccgctgcct gttcaaggta aaaatgggcc agccccgcca ccgcgccttc
     1621 ctgcgcgcca tgcaggatcc cgagctgcgc cgcatcgtgg aaaaatatga actgaccctt
     1681 taccaggata cccgcaagaa ggaactctac aagctgaagg aggaaatgtt cttcaccgtg
     1741 gatgaaaaaa cccacgacgc cgacctgatg gaaaaaggcc gggaagtcat ctcccccggc
     1801 catccggaag atttcgtgct gccggatctg ggaaccgcct ttgcggaaat ggacgaagat
     1861 ccgcgcctga cggaaaaaga caaactgcgc cgcaaaaatg aactgaccaa acagcttgac
     1921 gaaacgggag cgcgcctgca caccacctcc cagctgctga aggcctactg catttacgaa
     1981 aaagacgtgg aatacgtcgt caaggaaggc aaggtcatca tcatcgacca gaatacgggc
     2041 cgtgaaatgc cgggacgccg ctggagcgac ggtctgcacc aggcggtgga agccaaggaa
     2101 ggcgtggaag tggagcggga aaaccagacg tacgcgacca tcaccatcca gaactacttc
     2161 cgcctgtaca aaaaactggc gggcatgacc ggcacggcgg aaacggaagc cgcggaattc
     2221 catgatattt acaagctgga cgttcttccc atcccgacca accgcccctg catccgcaag
     2281 gatcagaatg acctcatctt caaatcccgc cgtgaaaaat tcaatgcggt ggtcaacaaa
     2341 atccaggaac ttcacgacaa aggccagccc atcctgatcg gcacggccag cgtggacgcc
     2401 tccgaaacgc tctcccgcat gctcaagagg gccaaaatcc cccatgaagt tctgaacgca
     2461 aaaaaccacc agcgcgaagc ggaaatcgtg gcgcaggccg gcaagcgcgg agccgtcacc
     2521 gtctccacca acatggcggg ccgcggcacg gacatcaaac tgggcgaagg cgtagccgac
     2581 ctcggcggcc tcttcgtcct gggtacggaa cgccatgaat cccgccgcat tgaccgccag
     2641 ctgcgcggcc gctgctcccg ccagggggat cccggcgcct cacagttctt catctccttt
     2701 gaagacgacc tgatgcgcaa cttcggcgcg gcggaacgca tgaccaagat gatggaacgc
     2761 ctgggcgtag ccgacggtga agcgctggaa cacagtttcc tgaacaaatc cgtggaatcc
     2821 gcccagaaac gggtggaaca gcgcaactac atgtggcgca agcacgtgct ggactatgac
     2881 gacgtaatga acaagcagcg cgaaatcgtt tacggatacc ggaatgaagt gctttccacg
     2941 gaaaacccgc gcgaaatgat ttacgatatt ctggaagaag taatcgccac gcgcgcccat
     3001 gaattcctgg acccggatgc ggaaggcgtc acccacccgg acgaattgct cgcctggatg
     3061 aacgcctcct tcccgctggg gctcacggcg gaggccgcca agctggaaga acggcccata
     3121 gacgacacca ttgccttcct cattgacaag gtcaagtcca cttatgaaga caaggcttcc
     3181 cgcgaacgcc cggaatacct ggaccacatg gaacgccaga tcatcctggg ggccattgac
     3241 aaaatgtggc aggaacacct gtacaacatg gactccctgc gggaaggcgt gcgcctccgc
     3301 gcccaggggc agaaagaccc cctggtggaa tacaaatcgg aagcttacga tctcttcatc
     3361 accctgatgg aaagcatcaa gagcgaagcc atcagcaacc tcttcaagag caccaccaac
     3421 ctggacgcct ttgaggactt cctggccagc ctgccccagt ttgaatcttc cgacgaaaac
     3481 caggaaggcg gaaccagcct gccggaaatc ggctttgacg gcatgccttc ggacctgctc
     3541 tccgccctga gggaacaagt ctcccgggca cgtgaacaac aatccgcccg gcaggcggaa
     3601 ccggaacagg ccccggccgt catttccgac gccaccacca ttggggaagg ttacaaacca
     3661 gccgtggcgg aacccaaact ggtcatgccc aagcgcaagg tcagcgtcgt gctccgcaag
     3721 gaagaagccc cccaggcgcc ggcagaaacg ccggtccccg gagaagggga agaaattgcc
     3781 gtcactctgg actcccagga ctttgcggaa accatggaca accgggattc cgcggatacc
     3841 cgtacattct aaaaaaagcc gcgcatcact tacaggccgg gggaaacggg aatgtccgtt
     3901 tgccccggcc ccaaacttcc ggtccgcctt ggctacgctc cctcccaaca aatccgccaa
     3961 tgtccgttcc gtgctggagt atgttccgta cttcagggat aaaatctttg ccgtgcacgt
     4021 ggaacgcccc ctggtggatt ccggggaatt ggtggatgcc ctgctggacc tggacgtact
     4081 gcaggaaata ggcgtcaggc ccgttctgat tgtggaaggg gcggacgcct ccgcgctgta
     4141 cgaacacacg cgcgtctgtg aaatgcgctc cgccctggtg gaagcgccat tgaagggcgg
     4201 ccagcttgtc cgggaaagag tccgggaaat tctggggcgt caccaaattc ccgtggtggc
     4261 ttccggccgt tccggctcct ttgacccgga atccgtccat atggccttca gtctgggggc
     4321 ctccaaatac atcgccctgc tcaatgacca caaggttccg tccctggacg gannnnnnnn
     4381 nngacgcccc attgccgcca ttctggaatc ggaagtagcg gagctggcag gaaacgtaac
     4441 ccaccgcgaa ctgcttgacc aggcagcgga agcctgccgg gcgggaatcc cccgcgtgca
     4501 tctgctggac ggaaaaatgc gcggcgtgct ggtggaggaa ctcttctctg aagaaggcgt
     4561 aggaaccatg gtccacacgg actcctaccg ggaaatacgc ccgctgaagg aagaagacat
     4621 tccggaactc ctctccatga tcgcccgttc cgtagtggac tccaaattgg tcaaccggaa
     4681 ctatgaagac attgccgcca gaatagacag ttactacgtg ctgaccctag acgacagcat
     4741 cgtgggctgc gttgccgttt atccctaccc ggagcaccat agcgcagaac tgggctgcct
     4801 gtacatcaag caccgccatg aaggccgcgg ctatggccgc gcactatgtg aatttgccaa
     4861 aagaaaagcg gaggaaatgg gaatggaatt catctttgcc ctctcccaaa gcgccgtcca
     4921 ttacttccgg gaccgcatgc attacgcgga attttcccgc gacagcctgc ccccggaacg
     4981 cctgcgcact ctggaactca gcgggcgcaa atccggggtt ttcgggttaa ggctgaaata
     5041 gttttctatt tacctctctg ccccgttcct gcttatgggc ggaatcccct gcaccaaatg
     5101 gacgggtagc tattcccgtc tctcgttaaa gggtatatag taaattcggg aaaggtcttc
     5161 catttttctt ttcctcctga aattttctgc ccgccgaaaa aaatatcatc aacaataatt
     5221 tgataataaa aatcttataa aataaccaca taactaaaaa agaattatgc ggatttttaa
     5281 tccgtccccg gctacggctt ctccgagcct ttcgggttta gctgctctga tgatgcgctg
     5341 cagcatctaa aacccatttc cctcccctcc gccgcatttc caaaaacaat gcggtttttt
     5401 attttccagt acctgtccgg tatcggagcc ttcacttcat gcggcatcct gaacggcaaa
     5461 atcctgagaa aagaaaacgc agcttctcac aagcgggatt ccaatccaac attcatatcc
     5521 ccgtgtggat atatgcaccg gaataacagt actccacggt tttccagagc ggaaagtgcc
     5581 ggggggaggc ttccaattgt caatcaagct gtgcatcatt gtgcatgctt ttttgaccat
     5641 atgataccct ttttcactgt tttccagcat gatgtgcaat atatgcacta aaagtaacaa
     5701 gccccttaaa ccgttggatt taaggggctt tgaagatggt agcagggagg tgatttgaac
     5761 acccgaccaa aggcttatga gtcctctgct ctaccactga gctaccctgc cgttaagtgg
     5821 ttgatgccgt aaggcgggga aatatttaac ccggatcgga aagaatgtcc agattttttt
     5881 atggatcagg agcattttat cacggcaacc cgccttcccc tccagggaaa tcattacgga
     5941 tgaagcattt ttcactcaac cgtttcatac agcctgtgtc ccatgccgtt tcctgcatgc
     6001 gggcatttac ggcacaggac attatgatgc cttctgtccc atgccattgc cgcagcagga
     6061 caggaaatag aggcccataa agattttcgg gtccaccact tttccctgtc ccatctgctc
     6121 catcagccag tcatgcacct gaggaagagg aatgcggtgc acagtaatat tttcgtgggc
     6181 cacaccgcct cctgccgata ttcgatgcag gtcttccgcc agatagaagg ataccatttc
     6241 cgtagtcaat cctggggaag aaggccccgc aaaaagatac gtccaggagt ccgcccggta
     6301 tccggtttcc tcttccagtt cacgccgggc ggattgcagc gtgctttcct ccccttcatc
     6361 cccggaaatg cccgcaggca gccctacggt ccgggcatgc aggggaacgc ggtattcttc
     6421 caccagcagc agttctcctt ctttcgtcac ggccacgatc atgacagctc cgctggcatt
     6481 aacccggcgc acgtattccc acctcccctc tttcagaagt tccagaaatt tcccccggta
     6541 gagagacagg catgcttccc ctggctgttc catttgttcc atgatgcagc cacgttaggt
     6601 ttccggacgg ccgttgtcaa ccatgcggac cgcctccctc ctgcatcttc ccggacagtt
     6661 ccgttccggt cccnnnnnnn nnntgcactt ttcgtcaccc aggccagtaa caaaacaaca
     6721 acacattgat gatgagtaat taacaatgtc atacagaaaa tatactcagg aggactaaat
     6781 tatcggagga agcaagaaca aaatattttt tcatgtggaa cccccgtgga acgtagagcc
     6841 gcagaacgat gactcgcgct gtttcaaaaa gatatttttt cctcagacgc aaatcagtaa
     6901 tttggcgctc ccgtcaaccg gggtgttgag ctgaaagcgg atgagttcgc cttctccacc
     6961 ccgatggacg ccacgagctg tcacggctgc ggatgaacct gatccggttc catccgcggg
     7021 cggcctcgta gcgggacgga acgccttacg gctatttcta accagcaaca tcttttattt
     7081 ccaataacaa tgacgccgcc atcagaactg tcagccacct ggtccaaaat cacgggagcc
     7141 ttgcaaacaa tcatgtcccc ggaggtttat ggattgtggt ttcccaagtt ttccctgctg
     7201 gaggattcag ggaaggcgct gacgctggta tgcgacgatc ctatggccgc cctctgggtg
     7261 gaaaacagct atacgccgga gctgaagcag gccgccatgc tggctctggg cacggaacgc
     7321 cagatcaagt tcgtctgcac ggatgaagtg gcttccatgc ccgctgcaga aggccagccg
     7381 caaaaagctt cttcccgggc caaaacgccg caggcggcgg attccccggc cgcaccggtc
     7441 aaaaaacagc gtacaccggc cgccagggca tgcctgaatg acttatatac gttcgattcg
     7501 ttcgtggtgt atgaggacag ccgttttgcg taccaggccg gactgtccgt cgcccagtcg
     7561 gaacgggccc tgttcaatcc acttttcctg tacggtaaat ccggcgttgg caaaacccat
     7621 ctgctccagg ccattggcca tgaggtgctc catcaggatt catccacaaa tgtggtgtac
     7681 gtcacgggcg aacagttcgc caatgaattt attgacgctt cccgaaccca gaacggacaa
     7741 agcttcacca aactgcgccg caagtaccgc aaggcagacg tcctgctggt ggacgacgtg
     7801 caatttatct ccggcaagga aaaaacggtg gaagaattcc tgcatacgtt tgacgaactg
     7861 ttccatgccc acaaaaccat cgtgatttgc gcggatgccg ccgcatgcga tatttccaat
     7921 ctggaccccc ggctggcggc gcgcctggaa tcaggcctta cggtagaatt gaaccttccg
     7981 gacgacaatg cacggctgga gatcctgcgc agcaagcgcg accgggccgg catgaacgta
     8041 tccgacgaaa ttctggagtt tctggcaagc cgcatccaga agagcgtgcg ccgcctggaa
     8101 ggagcactgc tccgtgtagc cacctttact tcccnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn atgcctgaca tcgccaaaat tgagcagctt ttacgcgaca
     8221 tcctgcggga agaaacaagc cgcatcctga ccgtagattc catccagaag cgcgtggcgg
     8281 atttttacga actcaaggtc agcgacctga ccggcaagcg ccgccccaac agcatcgcct
     8341 ttccgcgcca gatcgccatg tacttgagcc gccgcctgac ggaacgctcc ctgaaggata
     8401 tcggccaggc gttcggcggc cgggatcacg gcaccgtcat ccatgcgaac aagctggtcg
     8461 cctcccgtat ggaagaggat gtgcgcgtga gggatatcgt ccagaggctg gaagaggagt
     8521 tgagggattg attttccttc cctccagccc tgctggaaaa gcatggtccc gttgaatgcc
     8581 catggctttt caagcggaag aagcgcttcc gcggcgcagg ggagaataag cttgaggcga
     8641 aagcggtttg gattagaatc accctgttcc cattgattcc atgatccacg ccgaccagct
     8701 ttccaaagag tttggccgct ttcaagcggt aaagaatgcc tccttccaga ttgaaaaggg
     8761 ggaaatcgta gggttcctgg ggccgaacgg cgcaggcaag accacgactt tgcgcatgct
     8821 gaccggctat ctgcccccca cttccggcac agccacaatt gccgggttcg acattgtgaa
     8881 aaatcccctg gaggctcgca aacatctggg ctacctgccg gaacacgtgc ccctttacga
     8941 cgaccagcgc gtcacggaat acctgaaatt ccgcgccagg cttaaaggca tctcttccag
     9001 gcagatctgg cctgccgtct ccaaggttgt agaacaatgc ggcctggatc ccgtgcgccg
     9061 caagatgatc cgcacccttt ccaaaggcta ccgccagcgc gtaggcctgg cggacgccct
     9121 gctgggggaa ccggacctgc tgatccttga cgaacctacc aacggtcttg accccaacca
     9181 gatacgccag atacgcgaac tgatcaaagg tctggcggcc aaccatacca ttattctttc
     9241 cacccacatt ctcagtgagg tggaaatgat ttgcaacaag gtcatcatca ttgaccaggg
     9301 aactatcaag gcagcggata ccccctccaa cctgacggcc aatctacggg cagccggcaa
     9361 gattactctg gaattccggg gagacctggc cctgattacg cggaaactgg aaaacctgga
     9421 acatgtgaag aaggtcatcc atgaaggaac ggacgccgac ggctggcata cgctgaccgt
     9481 acgcgcggag gccgggacgg atacccgtga aaaggctgcg cgcctccttg cggaacaggg
     9541 ctgccccctg cgccatattt accgccacac ccccacgctg gaagaagtgt tcgtggaaat
     9601 gacccgtaa
//
LOCUS       DAWEMX010000098         9607 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_2969_length_9607_cov_12.694095, whole genome shotgun sequence.
ACCESSION   DAWEMX010000098 DAWEMX010000000
VERSION     DAWEMX010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2969_length_9607_cov_12.694095"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2100 a         2692 c         2716 g         2099 t
ORIGIN      
        1 gttcaccttt cgcaggacgg cgagcgtttc cggctcaacc tccatgctct tgatggcctt
       61 tgcaacctct ttcggcacaa aggcgtcgcc ctcatcattg acaaagggct tgtccttatc
      121 ctcctcgctc agctcattca gcaaggattc atactctgcc gcgagttcac tcaagcgggc
      181 ttccctggcg ttcagcctgg ctaggtctgc ggcgagcagg gtttgctgca ccagggcaaa
      241 gggaataatg tgtccaatcc agccgtcctg cacctcctgc tttttgccgt ccttggtttt
      301 aacgaccatg ttggggtcaa cctgtttgga agaagcaaag ccctcagtcc tgataatctc
      361 caggtccacg gcaatctttt tccactcatc atccagcaac tggtaggctt tgtatttgtc
      421 tatcagggga agcccgttca ggcgagagaa aacatcatca ctgatgatgg atttttctct
      481 ggtgatattg agcgtttcca taccatcgag caatcgggtg gtgaggaatg cttcaaaatc
      541 agcaaaagag gaagcgtagc gagcggtaaa tgccgtaaca tcagcggcgt caagaacgga
      601 cgacttgagg ttagccacgt tcaagcggca atgagcctcg ccggaagcgg tgaacagagc
      661 atctttcaga tgagggaatg ctgcccagta ctcatgaaaa tccgccagtt cagacaaggg
      721 gatgccgcca aacatggtag cgaaaatatc ccaacgttcg tttttgtctg aggaatcgac
      781 atagcgcggt atattgaggt tatagtcatt gccccggatt tcatcaaggc ttaccacctt
      841 ggcgaacttc tccacatcgg cgcgggcaga aaccacatcc acaatcttct tgatgtcgca
      901 cgcgcggagg acattgttct tgcccacctt ggcaaagccc ttggacgcat ccacaatcag
      961 cacatccgta ttttcccgtt tctgcttgag caccataatg atggtgggaa taccggtgcc
     1021 aaagaaaatg ttggcaggca agccgatgat agcgtcaata tggttgtatt ccaccagatt
     1081 cctgcggata gccccctccg taccgccacg gaacaggacg ccatggggca gtacaatcgt
     1141 catgatgccg tcaggcttga ggtggtatag gtcatgcagc aggaatgcat agtcagcctt
     1201 gcctttcggg gcaagcccaa agcggtaacg cgggtcgctt tccttgtctg cgggattcca
     1261 cgattgggaa tagggagggt tggagacaac agcatctaca tagagcgggt cataggtgtt
     1321 aaccgggtcg ttatcatcaa aatacggcca atcttcctcc agcgtgtcgc cgttgcgcgt
     1381 cacgatattg ttcggcaaaa tcccccgcat taccagattc atgcgggtca ggttgtaggt
     1441 gttttcctta agctcctgag cgtagtattt gatgttgtct cccccgccca tgtaacgcgc
     1501 cacgcacttg ccgatattga taagcaggga gcctgaaccg ctggtggggt cataaatctt
     1561 gatttgctgc ctgtctttaa ggtgggcagc cacgatttcc gacatgagga gcgaaacctc
     1621 atgcggggtg tagaattcgc ccgctttctt gcccgcgttt gcagcaaagt tgctgatgag
     1681 gtattcgtag ataaacccca ggacatcata atcctgccgc ccatccatgg ggatatcctt
     1741 gatgagatgc aagaggtcgc ggatggcttt ggtgcgggca ccggagtttt ccccaagttt
     1801 ggagaggcct gtttccaagg tggcgaacac cttgtcaaag acctttttgt gagagggatt
     1861 gatcaggcgg ctgaaagcag aaagagcctc cgtcacatca gcggcgttga agtcgctgcc
     1921 cttgtcaatc catgtagaaa agaggtgttt ataggagatg aagtaaccta cattgctgcg
     1981 gatatagtca accgtctccg cgtcatcttc cgtaacatgg ggcaaatcat cgtctgtcca
     2041 gtcgttctct ttcaggaact tgacttcctt gtcggaaagg aacttgtaga agatgaagcc
     2101 gagaatgtag tccttgtatt cattggcttc aattttggag cgcattttgt tggcagactc
     2161 ccaaatcttc gatgcaagct gttgtttgtt catagacagg gttgaacagt atacacatat
     2221 cttcccaaaa gtccagcgac aggcccatca gcaattccat tttttccgct tgcagcgtgg
     2281 tctttcctca ttcgttctgc gtcaaggaag catcgagcct cgttaccacc gaattgacag
     2341 cgtttaaacc acccctgaaa aacagcgcgg ctgccgggtt tccatgtttt ttctttaccg
     2401 ggcggacaga tagcccaggc caatggcaag gagccccccg gcaaagctga tgagagtgta
     2461 aaggaagaag gcggagaagt tcccttcctt caacaacagg aaggattcat tcatcagcgt
     2521 ggaaaaggtg gtgaaaccgc cgcacaggcc tgcggtaaga aagagccgcg tttccgtatg
     2581 caccaggctc cggtcgaaca aaccgtagag gcagcccagg ataaaacatc ccagcaggtt
     2641 gactgccgcc gtgccccagg ggaacgaact tgccgaagtg acctggacga accgggccag
     2701 caggaaacgc aatacaccgc ccgcaaagct tcccaggccc actatcatca gcattttcat
     2761 catatagcgg ctttttctat gttttaatgg ggaataaagt ttcggagcag ctgccgaaat
     2821 ccgccgggaa agacggactg cggcttcttg tccatgaaag ggttgctggc tgcgttcccg
     2881 aaaactggtt tggaaacgtt cgtgtcgcct ggtgacgccc tttaaccgga aacaagactt
     2941 ctggcctgta tgccggcaga acctatttct tttaagcgtg tgcgggtaaa ataccctgtt
     3001 ttttccatat cgccagcctt tttgttcttg attgcagggt aaaaccgtat ttcctttttt
     3061 ccatgtccct gccccttctc gttaccggaa ccatcggaat tgataccatc ctgactcccg
     3121 gcggcagggc ggacggcgtt ctgggaggtt ccgtttcctt tgcggccgtg gccgcccttt
     3181 tgtttgccga ccgcgtggat gccgtcagca tcatcggaga ggattttccc gcccattatg
     3241 aagcggccct tgaggccaaa ggggtgtgca tgaaaggagt agaacgcaag aaggggcctt
     3301 cttttgcctg gaccggggaa tattttgaca atatgaacaa gcggagaacc gtttgtgcca
     3361 atgatgccgt gatgctggaa tggaatgtgc acgttccgga gcctttgagg aaccatccgg
     3421 tgctggtttg ctgctgcatg gttccggccc agcagcttaa gtgcatggaa caatgtccgg
     3481 acgcggcgct ggtgctttcc gattccatgg acaagtggct gcgccgccag ccggaattac
     3541 tggatgccgt gatcagccgt tcccatatca ccatgatgaa tgaggatgag gcgaaggaat
     3601 atgcccaggc gtccaccgtc atggaagccg gggaattcct gctttccaaa ggcgccgcat
     3661 acgcagtggt gaaacagggg gaatacgggg ccactcttct gggacgggat gccggcggga
     3721 atctccggat tttccgttgt cccgcctatc ccctgaaaac gctggtggac ccaaccggcg
     3781 cgggggatac gttcctgggg gctctggccg gctatttgtc caccctgcct cccggccttc
     3841 ctccgtttga ggaagtgaag agggggattg tgtacgggac ggtggcggcc tccttcacct
     3901 gcgagtcctt ttcggcagac gcccttcttt ccatgacaaa agagaccttt cgccgccgtc
     3961 ttgaagagta tgcggagatg tgccgcctgc cagacatccg gctctcttcc gcaagtgcct
     4021 gttaagacgg aatcagacct cctgaaaaag aggggatgcc gtctttgatg gttcgttttc
     4081 cggtttccgt ttatgcggac tgtccggaat ttccaggtaa aaaaggcccg gacgcggaac
     4141 atgaaaaacc ccggcgtggc ttgcacggcc ggggtttccg taaaaacagg ggatgcagtc
     4201 ggcaggatca agcatcctga aggccgaatt taagctcggc ttccgaaacc acctggccgt
     4261 tcaccagaca acgagccgtc gcctggccga tgtttccgcg catcttggtc agttccgctt
     4321 caatgatgag cgtatcgccg ggaaccacga cgcggcggaa tttaaccttg tccgcactca
     4381 tgaagtagcc gagcttgctg gcattgccgg gctggcgcag catgacgatg gaggctacct
     4441 gggccatggc ttccacctgg agaacgcccg gcatgaccgg atgtcccggg aagtggccct
     4501 ggaaaaagag ttcattcatg gtcaggttct tcaatccccg gcacttggtt tctccttcaa
     4561 aaccgataat gcggtccacc atcaggaagg gatagcggtg cggcagaagg ctcatcactt
     4621 cgttgatgtc cagcacggcg tctccgtatg gaacattgac gggattgggg gccatctgct
     4681 ggttgcggtg gtgttccttt ttgagcttgg ccgtaagttc ggtgttcggg ccgtggcccg
     4741 gcttgatggc gatgacgtgg cccaggatgg gtttgccgca cagggtcagg tctccaataa
     4801 ggtccatggc cttgtggcgg gcaaattcat tgatgaaacg catgggctcc ttgctcataa
     4861 gctcctcgcc gcggatgacc acggcgtttt ccaggctgcc gcccttgatg aggcccttct
     4921 ccagcagggg cttgatgtct tcgtagaacg taaaagtgcg agccggggcc agttcctttt
     4981 catacgtttc cggcgtgata accgagtcaa agtactgggt aatgcggttt tccggcccca
     5041 cgcaggtgac ggaaacgcgg aattgcttgg aggggagaat ggtcaggatg gaaccgccct
     5101 tggtttcaat ggtaacggcc tcgcgcactt ccagatagcg gcgcggcacg tccagttcca
     5161 cgatgccggc gctcttgatc agttctacgt agggggctga ggacccgtcc ccgatgggcg
     5221 gttcattggc gtccatttca atgacggcgt tgtcaatgcc catgcccgtg agggcggaga
     5281 gaatatgttc cacggtatgc actttgacgg aaccttcagc caggctggtg gcgcgttcca
     5341 cagtctgcac cttttccacg tccgcgctga tgaaaggctg gtccggaatg tccacccggc
     5401 ggaatttaat gccgaagtcg gcaggagccg gtttcagggt cagggtaacg ggctgtcccg
     5461 tatgcaaaga ggtaccggcc agggaggccg gagagccgac agttctttgg tttccacatg
     5521 ccatgcgggg atgctagtga atgagcttcc gtttggaaag cacgaaatca cggattttcc
     5581 tttaaattcg gggaagaggc ggcattccgg cgtgtcatcc cggaaagaag aggataaagg
     5641 gcgcttgaaa aaaagccgct cacccagtag tgatgttcca tgcacatcac agcaggcctc
     5701 cgcatttccc gtgcgtttat tctcggcgcc gggctgggta cccgcctgcg ccctctcacc
     5761 ggcattctgc ccaagccgct gattcccttt ttccatgagc cccttattct gcattccatg
     5821 cgccgatgct acgattgcgg catacgggag ttcatcatta acacccacca tctggcggca
     5881 gcctgggaca aggtgttccc tgaacattca tggaacggct gtcccgttca tttcagccat
     5941 gaacccgtgc tgctggattc cggcggcggc gtcaaaaaga tagagcccct ggcttccgcg
     6001 gaagaaccgc tgctggtggt gaacggagac atggcggcca catttgacct gggccgcctg
     6061 ctggaggagc atttgagccg ccgccctccc gtaacgctgg ccctccgcac ttccggagac
     6121 aagaagaacg tgggatttga tttttcttcc ggcctggtga cggatatgag gcacgccctg
     6181 ggccgcgacc ccggatcctg ccaattcacc ggcgcgtact gcatggagcc ggaaatattc
     6241 ggacgcatcc cttccgggga ggccgtttcc attatccccc tgtttctgga ttacatccgg
     6301 gaaggacgcc tgcgcggcat tctggcggac gacgggttgt ggatggacat gggaacaccg
     6361 gaaacctacc ttcaagctca tctggatttc ccatcccccg ctccgcgcat ccacccccag
     6421 gccagggtgt ccccccgcgc ctttgtggac agccattgcg tgataggccc cggagctgcg
     6481 gtagaggacg gctgccgcct gcaaggctgc gttgtctggc cgggcgtatg cgtgccttcc
     6541 ggcacctgcg cggaacgccg gattttctat tgttccccca ccgattactg acgatgcttt
     6601 ttctgcgtct gcttcccctg atattgtgct tttccggcct ggttcacgca gaacaccgtg
     6661 tcttcaccag gaaggacgga tttttgtcca tgcgggacaa gctgaacgtg tattttttcc
     6721 gttctgatac gcaccgcctg ctggtaaggg atgaagggag cgtcaaaacg ccccggtacg
     6781 gctccctgga caaggccatg aggaaaagcc cctgcgtggc cggagtcaac ggaggattct
     6841 tcagcgcaga cgcggaggga actcccctgg gcctggtcgt ccaggacggc aaacggcttt
     6901 ctccgctggc cacggggtcc ttcgctgttt ccggtgtcgt ttacgaagga ggcagagacg
     6961 gattaaccct ggtccgcagt tccgtgctga ggcgcatgag aagactgcca gccatgcagg
     7021 cggctatcca aggggggccc tttctggtgg agaacggctc cgccgtaaaa gggcttaacg
     7081 cgcagaaatc cacctaccgc accttcattg ccacggacgg cggcaggagg tggtgcatcg
     7141 gcgtttcctc atccctgact ctgaaggagc tggcaacctg gctggcggct cccggggcat
     7201 tgggaaattt ccgggtggaa acggccctga acctggacgg cggctcttct tccgccttct
     7261 ggtgccatga aacaggcatt tcctaccctg cgttcaaaca agtcaggaat tatctgggcg
     7321 tagcccccgt ggaacgccgg taaacgccgt tctccacggg accatggacg cgtttccctg
     7381 cgcctatcct gcgcagcaac agagccgcga acaccaggaa acaaaccagg acggcccagt
     7441 cgcccagcat ggcgtacagg gttattttcg tacagcccac aggcaatgtg gcagccatca
     7501 cccctctggt aaacggtgaa ccggaggaat cccgcaaatc cgcaataacc gcgccgttgg
     7561 gggccagagc cacgctcacg ccggtattgg cggcacggac catggcacgg cgcagctcaa
     7621 tacaccggaa cgccgcattg cgccaatgct gttcgttggc gcaggaacgg ttgaaccacc
     7681 cgtcattggt cacgttcacc atcaactggg gctcctgccg gatgaagcgg cgcacccagc
     7741 ttcccacaac atcctcaaag cagacaagag ggatgacccc caccgtaacg gagcttccgg
     7801 gacggatggg cacgggaacg gggctggagg aacacccggg cgtataattc agtcccatgg
     7861 cggttccggc ggaagcctca aaggcttttt ccagaaatgg gaatgtttcc cgcataggga
     7921 tgtattcgcc gaaggggacc agcatggcct tggcgtgcgg ccgggctgtg gaataatccc
     7981 cctcaaagac ggtaaggcag ttgtaagccc gcgccacacg cccttcatca gacagataga
     8041 tatcatccgt gcccgtcagc agaataaaat tgcatatccc gttccggaga gcctggacgt
     8101 aatcttcttc cgcgctcaga aagttgacat tgtcctggtt ggagaagcgt tctcccgtgg
     8161 catcacggta aaacgtggaa atggggaagg aactttccgg ccagattacc caggccggca
     8221 tatccaggga ggcttccgcg ccgtctttga ccgcctgttc cagagcgcgg ttttgaagat
     8281 ccagcattcc ctgttccgtc atgtccagca aagcccggta aatgctcctc cggttggcag
     8341 ggtcccattt ttccttctgg ctcagattca gctgcacggc catgacggga acggtgaatc
     8401 tgccgtctcc cgtggcttcg gcagactggg cggagtaacg ggccgtccag accacgccag
     8461 tgacaaacat ggttaaaaga accgccacca gtatgaaaaa atcccaggga acagtacgcc
     8521 tgccttcatg gataatcatg gttccggccc ggcggcaaac gcgccagagc cagacggaaa
     8581 aaacggccgg aataaaagcc agcgccgtta ctccaaggta ttccgcccac tgggccagag
     8641 gattgccgta aagggccacg cccagattgt tccagccgaa gcccggtatc agccagcccc
     8701 gcccccattc cagacatacc cataaagcgg cgcccgccag agcggcggaa gcgctgggca
     8761 gtatgtcgcc cagggcccag gttttccagg cggcccggcg ttcctttgcc ttcagcctcg
     8821 catcgggcag cggccgggcg tccggacgga agaacactcc catgaccaga gcccagaggc
     8881 cgggaaacag gcctccgtaa aaaatcatca ggggaatata gcccagcgtg ctgacttcat
     8941 tgacccacca gaaggaaatg ccgtaaaata ctaccccaaa catccagcca tacagggcgt
     9001 atgccagcat tccccttttc ccctcccgcc tgcggccgta ccacagggct gtcagcagag
     9061 gcaggaaccc tatccatacg cacccgcccc agtccaccgg gataaaggcg caggccatca
     9121 gaattccgga aaaagcggcc agcagcagcc ctgcccacac gggcaggaga cgagggaggg
     9181 aagcaggtgg gatattcatc gtgaaaagcc ttttattccg ggaacgggaa tgccgccatt
     9241 gtcccccatg ctgactcatt cctgcaacaa aaaagattgc aaaatttact caaatgcgga
     9301 aatagtgggc gcagttcata caacccatca tacacaatgg cagatatcga cgaaaaaaca
     9361 cctctaaacg tccccgggaa attttacgtg gacagttcct gcattgactg cgacctttgc
     9421 cgcgaaacgg ctccggaaaa ctttggacgg gatgacgatg aaggagtatc ctatgtcaag
     9481 aagcagccgg acaacgacga agagctgagc gcctgtgagg aagccctgga aggctgcccc
     9541 gtggaagcga ttggcaacga cggcgaataa tattctccat atattatttt aatcgcccgg
     9601 ggctgtt
//
LOCUS       DAWEMX010000099         9425 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3038_length_9425_cov_11.311099, whole genome shotgun sequence.
ACCESSION   DAWEMX010000099 DAWEMX010000000
VERSION     DAWEMX010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_9425_cov_11.311099"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6113..6122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8325..8334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2204 a         2761 c         2386 g         2054 t
ORIGIN      
        1 actgttccac cagcagggct gccgcctaga gacaatggcc ctggcaggaa cggcccgtcc
       61 ggaggatgaa ggggttttca tcaacgacga caaggaaatc cgcgagcatg aaatcgtggc
      121 gggcagcatt ctttcccgcc tgtcccccta cggcagcgtc accagaacgg cacgcagcgt
      181 gctgaacctg ggcaccctga tccacttcgt cacttacctc acactggaat ctgatgacca
      241 gcatactccg gcccactgga tacggctgct ccaccccacc cccgccctgg gttcccagcc
      301 ccgcacggaa aacacgctct cccagctgga cgactggcgt tcacgtctcc gctgtccgct
      361 tcacttcggc gccccgttcg gttttctgga aaatggagac tttttctgcc tggtaggcat
      421 ccgctccctg tactggcagg ggcagaggct cgcactctgc accgggggcg gcgtagtggc
      481 ctcctccacc ctgacgcatg aatggcggga actgaaactg aagagggata cggtcaggcg
      541 cagtttccac cttccatgac agttcctccg gacgcctcat tcccttaatg gaagaacatt
      601 attcctccgg ccagcagacg gaaagcgtgt caaatttatc cttcaccggt attttccaca
      661 cccttccttc cctcatcacg ccgcagcacg gcacgccgtc catgcatgca ctgacggaaa
      721 tacccgcagg caaaccgtgc aaaaccacct ctgcggactc atggaacgca cagggatccc
      781 cttcgcagtt ccaatccagc tccagggaat tggagccgcc gcggtaaagg aacccatgca
      841 cggcgcattc tccgttccgg tatgcatatc cgtcccctgc atcttcatac aaatgggaag
      901 ccacctcccc ttccggcgcc caccatacat ccagcgtcag cggcggccga ggcagttctc
      961 ccacgtattg ctgcaccggc caatggggaa ttaccgcccc tccgcggaca tacacgggca
     1021 ggaaagaaag ggggcacttc acccatacat cctttcctgt gtcctccatc aggctgtccg
     1081 tgtggtagga ataccagacg ccctccggaa tgtagaggaa ccgcccgcct tcctgcggct
     1141 catgaatggg aatgacgtac agatggtctc caaagaaata ctccgccccc cgccagtagg
     1201 tgtccttgtt ggtaaaacat tgcagggcca gggagcgcag cacgggcatg cccgtctccg
     1261 cgtaccggcg gaactgcgtg tagatatagg gaagcaggcg gtagcggccc tctatggcgg
     1321 ctttcacgta agaggtgact tcctgcccaa aaacccatgg ctcctgaccg ccgaactccc
     1381 cggaggaatg attgcggaaa aaaccgtgga aagaagccat ttgcatccag cggcaaaaca
     1441 attccggcgt gggatgcccc atgaatcccc cagcatccgc tcccgcaaag gaaatgccgg
     1501 aagccgcaag ccgctggcac tggaaattgg ccagcttcaa atgttcccag ctggaccggt
     1561 tgtcccctgt ccatgtggcg gcaaagcgct gcaatccggc aaaaccggat cgggacagca
     1621 ggaaggggcg ccggtccgga gcgtgacgct tcatgcccag ccaggaggct tccgccatgc
     1681 actgcccgta aatattatgg gccttttcat gggaacaggg catgccgtca tattcatgcc
     1741 gcgtatccat cgggaaagtg cggtccggaa aaacgacagg ttcgttcatg tcgttccaga
     1801 ggccgcgcac gccaattttc ccaatatccc tgctgaacag atccgcccac cagccgcgta
     1861 cggcaggagc cgtaaaatcc ggaaaattgc aaagtcccgg ccatacttct tccgacaata
     1921 aattgccttc cgaccggcgg cagaaataat tgcgttccat cccctctttc cagaccggat
     1981 tggcggaatt gaccttcacg ccgggattga caatcagaac cgtcttcaca ccgtccttct
     2041 ccagggcgcg gaccattcct tccgcgtcgg gaaaattctg tttgtcccag gtaaatccct
     2101 ccttccgttc catgtaatga tggtccagat gcacggcatc gcacggaatg cccaggcccc
     2161 ggaaacgctc caccagattg tatacggcct tgtccggata atagctccac ttggactggt
     2221 ggtaccccag cgcccacaaa ggcggcaatt caggcgtacc ggtaaggcgc gtataagcgg
     2281 agatgatgtc cagaggagtg ttgtcgtaaa tgaaataata attcatcagg cccccgaagg
     2341 acccgaagga aagaaccttt tcatccgtag ctccgaagtc gaaatacgaa cggcacgtgt
     2401 tgtcaaacaa taggccgtac gccttccttt cccgcaaact gaggaaaaag ggaatacttt
     2461 tgtaaagggg atccgactcc tcatggaaat cataatggtc cgctccccac atggagaaat
     2521 acttgcccct caggttcaag gcgcacggct tgtccccaag cccgaaaaaa tgctccgttt
     2581 cctgaagatt tttccgtatc catacccggg cgtctccagt ccagtccttg ctctcccggc
     2641 cgaatccgcc ctcatccgtc agcaggggct catccgtggc aacatcgtaa aaatccacct
     2701 tttgctcctc cgtgcgaatg cggatgcgga gcagggatgt ctcaacgaca tgaaccccgg
     2761 cctcatcata ctcgcggatt tccgctcccg gcgcggaata tccggggctt acggcataac
     2821 tcggctcgtc ttccggcacc gctccgggga aatacgttac gcggacaata ccggcatcag
     2881 caagacacac gctgagagtt attttggaaa ccgtattcgt gtactcccat ttgacgaaac
     2941 tcccccggag aggaggtgtt tttcgtcttt cagggatgtt ggaaacggag cacattgaag
     3001 ccggacggaa gagaggatca aaagcgttca gtccagacta aatcaatttc atgacgaacg
     3061 caagattgaa ctaaagcagc acttttcttt tcattattag agtaaaatta ttcatattaa
     3121 tctagaccga acatgaaaaa tctggtttta ttgcgttggt gtacaacacg gcacgagtcc
     3181 gtatccttcc cggcaccgac aaaccacatc aaccgatgag cacaattcga gttcttacat
     3241 taggatggga attcccgccc ctggtcaacg gggggctggg cattgcctgc ctgggtcttt
     3301 ccaaggcgct ggcaaaaaaa gtggatttaa gggtgatcgt tcccaaggcc gacccttccg
     3361 tcctttttga cggattccag ctcaccggtc tcaataacgt ctcctatcgg gaagtggagc
     3421 aagtggaccg gaaatattcc tatgacagct ttgccctagt ggagcgcgcg cccattgaac
     3481 tggaccccta caccaccgtg gaaggagaat ccggcgtggt gcagttcacc aaggaaggcc
     3541 gcatcacctt ctccaaaaca catgaagccg accttcagct gttcaggaac aaggaagacc
     3601 tttacgccgg agacctggcc ctgaaagtca ttcaattctc aaaaatagcg gtaaaagtcg
     3661 cccttcagca ggattttgac attatccacg cccatgactg gatgacctat ctggctggcg
     3721 tggaagtgaa aaaagccacg ggcaagcccc tggtggtgca tctgcacgct tcccagtttg
     3781 accgtgccgg agcggatgcc cgcggctgga tttacgacat tgaaaaattc ggcatggaac
     3841 aggcggatgc cgttatcccg gtcagtaaat acacgggaac catcgccagc gggcactatg
     3901 ccatcgaccc ccataagata tttcccattc ataacggagc ggatccggtc aaagtcttta
     3961 aagggaagaa aaaattcccg gaaaaactcg tcctcttcct gggccgcctg acggctcaga
     4021 aaggtccggg attcttcctt cagattgccg ccaaggttct ggaacagacg gacgacgtac
     4081 gcttcgtcat ggccggtacg ggagaaaaac tccgccagtt gatcgaatcc ggagccttca
     4141 agggcgtggg cgacaaattc cacttcaccg gcttcctgaa caaggacaaa gtcaatgaac
     4201 tcctctccat cacggacatc tactgcatgc cttccgtatc ggagcccttc ggcctttctg
     4261 cgctggaggc ggcccaattc aacattcccg ccgtgatttc caagcagtcc ggcgtggcgg
     4321 aagttatgaa gggagccctg aaagcggatt tctgggacgt caacaagatg gcggaacata
     4381 tcgtccatct ctgcacggat gaggaattgt accggaaagt agtggaacag agcacggagg
     4441 acatcaaggc ttccacctgg gatgccgccg cagacaaggt tatccgggtc tatgaacatg
     4501 tgctgaaccg ctaaaaacgc ctttttaccc tactccataa ctctcagcct tcttcacccc
     4561 gtccatgagc aacatttccc tcactttcgt tgcgcatcag cccaaccggc tgattcatta
     4621 tgacttcttc aaaattggag aacatgcctt ttatgaagat gatgatctga atgcccgcgt
     4681 gcttagcacg gtggcggaac gctgttattt cccggcaacc cggctgatga aacagcttat
     4741 tgaactcacc gggggcaaat tccgctttgg gctggcgctc agcggagtca ttctggaaca
     4801 ggccctgtac cacaggccgg acctgattgc cgcattcaag gatctggcgg aaaccggctg
     4861 tgtggacttt ctggcaatgc cttattacaa ttccctggca tccgtttact cccctcagga
     4921 atttgccgaa cagattgaag aacaccggga gcttctcaaa aaactcttcc atcaggaaac
     4981 ggatgtgctg atgaataccg gcatgctgta ctccaacgcc attgcggcgc aggcggaaac
     5041 gctcggcttc aagggcatca tggcggacgg caacccggcc atgctcaagg gatttaccag
     5101 caatgaagtc ttcctggctc cgtgggtgta taacaccacc actatcttcc gcaacaggga
     5161 actctccaat gacctggcaa tcatgcggac tgatccggaa tggtcggaat acccgctttc
     5221 ccccaccact ttcgcggatt ggctgacgca ccagcaggga agcgtcacta ccctgtccat
     5281 ggactatgaa acgctggggg aacgccagtc tgacgctaca ggcgtcttcg aattctggcg
     5341 tacgatgatt ctcgcctgca tagacgccgg caaccggttc atgactccgt ccgaagtagc
     5401 gcaggaaatc aagcccgttt ccgtctgtga gtgcacccag gaaatgacct gctccacttt
     5461 tggaaccatg tcccattgga atggcaacgt catgcaggat gaagccctcc gcaaaatcta
     5521 ccgtctggaa aaaccggtca aggcagccca tgacaaggat ctcacccacg tttggcgcaa
     5581 actgcagagc gcggaccact tccactacat gcagaaggaa aactccttca cgccgtatgc
     5641 atccgccttt gacgcgtaca tttactacat gaacgctttg gcagatttgc aaatacgcgt
     5701 caaaagagaa tccggaaaac cgattcagcc ggatccggaa aaagcgtcct gacctcttct
     5761 cttccgaata ctgcgaaccc gggtggatta tagtctgccg ggaacatcac gggacggttc
     5821 ccggctgtaa aaactttgcg gttccggaat gcggtcttct ttgcccccct cctttggcta
     5881 ccataaaggg cctgagggat gtcacggaaa gctaatatcc caatgccaga cgcttcaatt
     5941 tgttgcgcag ggtaatcttc tgtgcggaag aaacgcgctc cacaaaaagg atgccgttca
     6001 aatggtcgca ttcatgttgc aggcatctgg ccagaaggcc gttgcagtca atcgttattt
     6061 ctcttccgtc aatcagaagc accgtagcct tgacaaaatc cggcctcacg acnnnnnnnn
     6121 nntattttca tcacgctcag gcagccttca tgaaaaggat gcatggggcc gtaaggttcc
     6181 agaacgggat tggcaaacat caagggcatg atgtcggaaa gctctttgtc ctctccgttc
     6241 actttaagcc aggtgacgga ctcttcctcc ttcggaatat caatcactac cagttgaatg
     6301 ggaatgctca cctggggagc cgccaggcca atgccctctg ctgcgtacat ggtttccagc
     6361 atattttccg ccaaggtttt caggctgtcg tcaaaatgtt ctacagggcg gcatttttcc
     6421 ttcaataccg gatttccata ctgtgctatt tccaataaca tcgggagggg gataatatat
     6481 atgttaaggc gcttaccgtg cgccgggctt gttgatttct attctggcag gaacctcacc
     6541 tgcgtcaata cccacagacg tcagcgcatc ccaaatgccg acaatgatgc caaactccgc
     6601 ttttttatcc acatcaagct gcagtttgac gcctggattt tcctttttca aggcttccag
     6661 ggcagcagcc agttcattca tctccaccag cctgccattc agggaaatgg cggatgtccc
     6721 cgtcacggtc agaacggccc gctgttccga agacggagtc ccctccatga attccgctcc
     6781 gggtacatct atcttaagca gggactgcgg cttcttccac tgcgtatgga caatgaaaaa
     6841 aatcagcaga atggtcaata tgtcaatcat ggggacgatg ggcaccccca tgctccttgc
     6901 tttcttgcgg taaaactgca tggccttgtc agcgttggga atgatggcag cggaaggaaa
     6961 ccagttctgt cagcaggact tccatggagg cggaaatacg ctccagcttg cgggaataat
     7021 aaacgtgggc aatcacggcc ggagtggcga tggcgagacc ggcaatcgtc gtattcagag
     7081 cctgggcaat cccctgagca atcatgccgt gactctcatc cattccgaaa acgctgaaaa
     7141 tctgcaccag gccgctggcg gttcccagaa tgccgaacat gggtgcgatc atcacaatca
     7201 tgtccagcag gggcagaccc gcctgaagcg ttacaaactc ctcgcgggcc ttcacctgga
     7261 tcatttcctt cagggaggct tcatcctcca gcggtgcatt caacgcatcc agcaccaggc
     7321 ggccctctac ggaatccgaa tcctcagcta ttctctccaa ttcagtgcgc cccacggcgc
     7381 cgcgggtgta ctgctccacg gccctggtca gcacggcggg gcaaatgagg ctcttcttca
     7441 tattgaacat gcggtaaata acggccgcaa taagaagaac ggaacaggct gccagcgggt
     7501 acatgaaaaa gccgcctgcc tggaagaata taatgcagtc tttgatcaag ttcatcatca
     7561 tatatctgtg aatggtattt gttgcaggat ggtaggttgt taaaaattga aatcgaaata
     7621 catttccaaa gaatccccca caagcatact ccttacctcc gggggcatcg ctggaagcct
     7681 ggcgagtttg atggcgagga aggtaaagga cttctgcacc tcgctggctc ccacgcggga
     7741 agtctgcctc atagacgaaa ttttgccatt cttgttaatc aggatgcgga tgcgcacagt
     7801 tccggtaaca atcaggtcgc ggttcaaatc gcactggcgg taccattgtt ccccgatagc
     7861 ccggtaaacg atcgcctggt acgttcccat gggcgtagct tccacatcca atgtcggacg
     7921 gcggccaaag ctgaatttgc cggtctgctt ggttttccgc tcatgtgtct tgaagccggg
     7981 ctggctctcc gcattgaaca tgggatcata caagggctgt ttctgaaccg gttttgtatt
     8041 gggcaccaaa tccagattgg gaagatttag gggaggattg tccgcccctt tcgcaatgct
     8101 tttggccaat tcctcaagcg gatccggctt ctccgaatct ccggggctgg gcaacactac
     8161 cgcatgggga agctgcatgg ccttttcctg tgcatgacgg atgttttgtc gggccgtttt
     8221 ttcctcttcc gatttgcctg gctctccctt cttgacggga gaatgagcca gggaaccggg
     8281 atgaccggca ggcggcatgg atggttccag gggctggtag ggaannnnnn nnnnctccgg
     8341 gggaggcgtt tttgttgccg tcccgttcat ggcttaaatc cccatcctgc ctttcctggt
     8401 taaaaaggac aatctctccg ttttcccgtt ccacgccatc ctgggcgggc atatccctgt
     8461 cgccgtccgg atccttccgg ccgccttccg cccgcgtgtt cctgttgctc tggaatttgg
     8521 gcacttccgg cctggtggcg ctttcctgct cctcattggt cttcacgacg gcaggcagat
     8581 attccgcggg ctcttgtctc cggctctcca gttgaggttc cactttcttt accatgcgcg
     8641 cgcttacagc cactttcctc cgcgccgctt cagccggacg tggggaagcc agccattctt
     8701 caggaaccag gcagctcccc gccagcaaaa acaggtgtgc cagcacagac agcaaaaggc
     8761 atgtgcccca ggaaacccgg aacagtccgt ttctccgccg ctgcctgaaa ctcatggagg
     8821 taaagttttc acggttccat gacgaatgca atgtagaaag gagacatgat gaaggacgat
     8881 tccgtaattt agtattgcac aagaccatgt gcaggcccat acttgctagc gaccaaacac
     8941 atgccttcct cctaaacggc aaggcccttt tcctatatac catacacctc gttaacaccc
     9001 atcctatatg gccaatctca acaaagtctt cttaatgggc aaccttaccg ctgatcccga
     9061 actgcgctac actcccaaag gaaccgccgt tacggacatc cgccttgcca tcaaccgtta
     9121 ctacgcgggc gacaacagcg aacgccagga agaaaccact ttcgtggacg tcaccctttg
     9181 gaaccgccag gcggaagttg ccggcaacta cctcagcaag ggccgcggag tctttgtgga
     9241 aggacgcctt caactggatt cctgggaaga taaagcctcc gggcagaaac gcaccaagct
     9301 gcgcgtgatt ggggaaaaca tccagctctt cccccgcggc ggtgaaggcg atatgggagg
     9361 tactccgcgc cagcagtacc agaatgctcc ccgttccaac aactacggac aatcccgccc
     9421 ggccc
//
LOCUS       DAWEMX010000100         9342 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3072_length_9342_cov_10.363842, whole genome shotgun sequence.
ACCESSION   DAWEMX010000100 DAWEMX010000000
VERSION     DAWEMX010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_9342_cov_10.363842"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1780 a         2560 c         2830 g         2172 t
ORIGIN      
        1 gaacggattg ttatgagaaa aacattgtgt ctcttggcgg ttttggcgtt ggttcctatg
       61 gttcatgctt cttcgttttc cattgacctg tccccgacgg gttctgggac tacaggtgtt
      121 tcttcttccg ggatcactcc tcaaaacgtc ttcggttata atgtcgggga atggcacggg
      181 acttgggaaa gcagtagttt aagtggtcag gtagggtttg acgaaggagt cctcaggttg
      241 caaatcggag ccgtggcttc caataatgcc ggaggaaatt ttgccggact caaatttgac
      301 atgccgtctg gggcggcttc cgcctccctg cagtttgata ttgccaaaac ttcttcttgg
      361 ggtggttccc ttgggaattt tgaatgcaaa tatatttgca atgtttatgg tttctcggat
      421 gatggagaat ctacggtcat aggaacgtgg acattggaaa acgccaagac gaacctgacg
      481 aacactacct cttccttttc cattagcttg gatttgagcg gaacggatga atatgcgtct
      541 tatggattga tattcaactc tatggaaacg tcaacattgg gagctggcgc tggaatggcc
      601 gctgatatta ccaatatacg ccttttgggg gaagttgttc cggaaccggc tacggcctct
      661 ctgggacttt ttggcctggg cgcccttctt ctgcgccgcc gcagaaactg atgacggtca
      721 tacagggatt gaccggacag gctgtgtcca agccggtttt gtgcgaagag gggatggttc
      781 atcccctctt tttatccgtt ttccacgcag ttttttctgg tttgatggac agggacggat
      841 aaaatggcgg acagtattta tggttaaggt gtttttcaaa agggcagccc atttgctgac
      901 agcgtttttt gcgggaggtg tctgtgtcct gcaggcggcg gagacatccg gcttcggcat
      961 agacctgggt aatggttccg gagcggcgca ggagggatgg accaatgtaa ccatgccagc
     1021 aacggcatcg ggaggcgcca atacatttgt tgccgttccg ttaatcagga atggtgcggc
     1081 ttctttttcg tcgtccgtgg cagtgggaag cctgttcgga aggctggttt ccctgaccgt
     1141 ctccgccaga tgcagttctg gaggttctta tacgctggcg gaacccaata cccggtatac
     1201 ctggtatgaa ggggggaagc acaggcacaa cggtacgaac gctgacgcgc cttcttcttt
     1261 tgaattcccc aatggctgcg aagctttcaa taccagcatg cgtctgagcg ccaacgggat
     1321 gccgtccgcc tccgccgcgg ccaggctttc cttctccgga ttccaggcgg gcaaggaata
     1381 tacggtatct ttcttttgcg ggcacaatgc gcctgcttat gaatccatga cgctggtcag
     1441 cggaactctg gtggatgtgc gggggcttca aagttccatg gggagccttt ccgggaatca
     1501 attttccggt ctttccaatt catcggaaca ggacgcttat ctggctgtgg agtggagggc
     1561 ctgtgcggat gaacagggca ggctggtgtt tgatgtgacc aaggaggcgg acagcagcgc
     1621 ttccggccgc atggaactga atgccgttac cgtcagtacg gacgattccc cggctccgga
     1681 agttccggat ccccggcctg tcagcgtctt gcacaataag gcgctcattt ttcttccttc
     1741 ttctcttgcg ctccccgttc cttcttccac atgggcggct gaaggccagt taacctgtga
     1801 attctgggtc aggcccaatg atgatgccgt cacgggaaaa atcatttctt tgggaaatgc
     1861 gtctgtttcc ctggaacacg gttgtctggc tctgtctgct ccggatgggg attccgtcgc
     1921 ttccgtgaaa gctgccggga aggaattggc tgcgggggaa tggtaccatg tagccgtctc
     1981 cgtcggggag gaggttatca gtctgtatgt ggacggaggc ctggccgttt cctcgccgcg
     2041 gtcgccttac ctgcaggcca tgaaatcagg ctggaaaggg attgtgctgg aggcggggtt
     2101 caagggcgct ctggatgaac tgcgtttctg gaatgcggct ctgggcggga aggaagacga
     2161 ctttttcttg aacggaccgt tgcccctgtc ccatcccagg tatgggaggc tggtgggctg
     2221 ctggcgtctg gatggggatt tccgtgatgc caagtggacg gaatttgcgg aaaagacagg
     2281 gagcgccttt gtgcgccctt accaggcggt tgcccctgaa gggactgaat tttccatcgt
     2341 cacggacaat gaaacgttcc gttacatgct ggtgacggcg tatgtgagga atgtgcatgt
     2401 tatttatgac tggccggcgc gcgcccatct catcaataat tccgatttga tctacatcaa
     2461 ttccgttacg ccggccgcgg acggttctct caattttcaa tatccggaca atgacgtgac
     2521 ggaaagctcc ggtgtggttc tgctgccgtc tgacggggaa tgttccaatg tcctggactt
     2581 ttccggggaa ggggcttaca tgaacgtggg cggaggtttg ctgggcggcg gcagttccgg
     2641 agcgtttacc gtggaagtcc gcatggcttt ggacggagac gggcaggagg cggtgctgtt
     2701 tgaaaatgat aacgtttccg tgaggctgga atggaaggaa gaccattaca gggtccgaac
     2761 ccaggcaggc acggataaag cctgggaggc ggatttgccg cctgtggaag ccggcaggta
     2821 tttctggctg gccttcgtca ggaattcttc tgcggatacg gcgtcctttt atgtggacgg
     2881 ggaggccgta agcacggccg ccgcggatgc gggggaaatg gaaggaactg ccaatgccgt
     2941 cataggaagg aatttggatg gcagaattga tgaaatgcgc gtgtggcatg aggcccgtgc
     3001 ggcggcccgc ctgggggcgg ctgtccagca cagctgggga gacaggctgt tggtgggccg
     3061 ctgggggaca agtggccaat ttggccatga tacagcttcc tgggtggaac atgtccgtat
     3121 cctccgcagg ctgactgaag gcgtttccgg aatgcggata cggctgggtg tctccggcgg
     3181 gaactggtcc gccatgctgt ctgatgcaaa tgcccgtaag gcttttgccg aaaatgtggc
     3241 ggaagtggta cgcaagcatc aattggacgg actggatctg gacttcgaat ggattgacca
     3301 gaacaatacg gccgcctgga ataattacgg agaattggcc agggctatcc gggcggcttc
     3361 cccggatatg ttttttacca tttccctgca tacctactat tataaattcc cggcggcctg
     3421 catgcgttat gtggattatt tcacgttcca gaattatggc ccccagattg atgtaaacgg
     3481 atatagcagc atgatttccg catgcggaac gtaccgttca tggggttatc cggactccaa
     3541 gatcatgctc agcgctccgt tccaggggac tccgggcgcg gggcagggcg ccgatatccg
     3601 ggcttaccgg gacattgtct cagcctgcgc cggagtgcgg gaggatcctt ctctggattc
     3661 cgccagcttc aattatggcg gaggcaaggt taagacgctg cacttcaacg gtgtggatac
     3721 ggtcaggaaa aaggcccgct acatcagtga acagcaagtg gctgggttca tgtactggga
     3781 tttgggaatg gatgtggcgg attccgccgg gaagaataac tactttgacg aatgctgcct
     3841 gcttcgggcc gccaaccgtt atgtttcctc caccgcttat ccggatactc cggccccctt
     3901 tgccctttct tctgcggggg aaaccgtccc ggccgggggc ggtgccgtgg cggtggaagt
     3961 gcagtcggaa gagaaggctc tgggctgggt ggtcgcagat tgtccggaat ggatttccgc
     4021 ctccgccgtt tcgggaatcg gccggactac agtcattttg acggcggcgg aaaacaaatc
     4081 cgctgacgga cggtccggaa cggtaatctt ccgttcttcc gacaaacagg aatgctctgt
     4141 catcataacg caggatggcg ccgaattgac gggctacgac aagtgggtgc aggactcctt
     4201 ccctccggat gctgccgcgg accggacggc tgcggatgcc gttcctgccg gggacggtat
     4261 ccccaacctg atgaaatacg ccacaggaca ggatccgttg aaaccctgcg ggagcgttac
     4321 gaaagtaacg ctggaagagg gggaggacgg atgcatgcat ctggtgctgc gctggcctgt
     4381 aaatccgcag gcaacggatg tgaagcatga agtggaagcc tccacggacc tggtcgcctg
     4441 gatttccctg ggagaagtgg aaaccgccgg aaagacggct gccgaatttt gggatacgga
     4501 acccgtacgg gaaagcggga tggaacgccg gtttctgcgg ttgaaagtga ctcgggaata
     4561 acttcggtac gtcctttctg aaaaaaattc cgttcctgtt cccgtttttt gagaaatcta
     4621 gacggtccgg cggggcatgc ccggctgggg agcgccgcgt gcccgtaccg caaccggacg
     4681 ggcggctgtt tgtacaagat actcgaatta caaaagcatt gcttcccttg aaacaggcag
     4741 gggtcgcttt aaaaaatcgc gtccgcttcg cacgcattat tttctgccgt ctgcagaaac
     4801 ctgcgctaaa atttgtgttc caccgccatt ccgtggcatt tccggaacgg gaaaggagcc
     4861 tatttcttct tcgtgttgag tttttccctt acccgttcca cattggcaag agcgtcttca
     4921 ttccccagct ctgccgcccg ttcataccac ttttgggctt cggccaggtt cttggctacg
     4981 ccagttccgc gttcatacat ggaccccagg ttgttcatgc acttggcgtc gtcctgttcc
     5041 gcccccttga tgtaaaactg ataggccagg gccatatcct gcggggtgcc gaggccttct
     5101 tcatgcaagt agccgagcag gaaatgcatg ccgggcaccg catgcgtctg gttctcttcc
     5161 agatcggtca gaatatccag cgccattttc gggtccggct ccagatcctc tcccttgatg
     5221 agcaggcgcg ccttctccac cagagcttcc ggatccctgg tttccgcagc tttcagtaca
     5281 taatccttgt acagttccct gttcggctgc acgccgatgc cctgctcgta atagcgggcc
     5341 agcctcttga aaccgcgtac gtcctccttt ttcaccaggg cggtggctgc cgtcatggca
     5401 gaggccagaa gctgctgccg ttccgcaggc tcctgcacat tccctgccac ggtttcataa
     5461 taattggcca gctcgtccag tgccgtcagg ctgccgagtt ccacagcctg ttcccagaac
     5521 ttcacggctt ccggattgat ggttttcggg gccggggcct ggcgttccct gccggacggg
     5581 gtcatccaag gaggaacggt tccggcatcc gccggagtat cttccccctt acccttctgt
     5641 tccggtttcc ggagccacgg cggagtgttg gcgtcccctc cgttcagggt cagcttgacc
     5701 tgctggcaag cggaagttga tccattgtcc agggccgctg tttcccccgt attctgggga
     5761 taggccgcct ggttggccag gaaaagcagg gaggggatat gcccccgtgc cgccagaccg
     5821 tgcatcatgg cgctgtaagg ctgcaggttc tcattgttgg aactccacat gtccgccgca
     5881 tccacatggc ctttcaaggc tccccggaac atccaggaat ccccctcggc catgcgtttt
     5941 tccaggccga attcataaat gagggcgcgg ccgagcacgt acatgccgca ggaatctccc
     6001 tgtccggcgg aaagccgggc ccaggccagg gcttccgggt agccggattc ttcgctcctg
     6061 taactgaata acatcaggta tgccagatcc gcctggctgc gggcgttccc cccctgagct
     6121 tttttacgga gggtggcggt cagctgttcc cattgttccg gcgtgacgta ttcggaaatt
     6181 cctttcagcg tgatgaaatc tcctgcggcg gcttttgcca ggcaatcgtt cagcagcgtt
     6241 ctggcaggtt ctgcggacgg tttgtccatt aagtccgggt tttccatttg ggccaggcgc
     6301 agcctggcca gggggtggcc ttcctccgcc gcctgcctgt accatacgcg catattttcc
     6361 cgcgcttcat gggccttccc tacggaatcc agtgtattgg ccagttcctc cttccacagc
     6421 tcgcccaact gtacgctgga atctgcgtcc ccaagctgag aggccagctc aagctcttta
     6481 tgtgtcttgg cgtagggggg aagctggttg agttttttct gggggaagga aagaggttcc
     6541 ttcaggtatg gcatgccccg ttcgtcatga agggacagca gatcaagggc cgccttgcgc
     6601 agctcctgtt cctgcggggg ctgcgcttcg gaaattaact gggcgtacca ggcacaggcg
     6661 ttattgctgc ccagtttcct ggcctgctcg aacaaaggct ggaggaggga gcttttccct
     6721 ccccctgttt tttccttcat gccggcggag aggtccgcca gcagagtttc cagatcccct
     6781 ctggagcgca gttccgccaa aagctttccg gcctgtgccg gtgcggcccc cttcgtcaat
     6841 tccgccagaa gccgcatggc ccccgcgtgc cccttttccg cagcccggga aagggattga
     6901 atggccgtat ccctccatcc cctgccgtcc ttgccgtcca gggcaagggc cagttcccct
     6961 atccgcgcca gaagaaccgg tttggcgttt ttccggtatt gcttcagggt cccgataacg
     7021 ttggcgggat tttccttcag ccaatccact ttgatgaaag cgagctggga cagcgcatcc
     7081 tggtcgccgg aagcggcctt cttttccagg ggggccaggt agcgtctcaa ctcggcttcc
     7141 ggaagatccg tgacggtgta cagggccgta acggcatcca tgtctccctt ttccgcaagc
     7201 ttgcggcagg cagccagcca ttccgcatgt tccggttgca tgggttgcgc cttgaaaatc
     7261 ccgaaaatcc cgtaaggatc cgtcatcatg agcttgggtg ccggatggcc ggatgccgtg
     7321 gccgcatcac ggaaaaaatg ctgcatatgt tccaggcagg ccagcggcag gtctccacgg
     7381 ttcaggtaat aaagccccat ccagtattcc ccggccagca tattggcccg gaagtctcca
     7441 gccgcggctt tgtccagcac ttccccgtac gtccagccgc cgtccatagg cagttgggag
     7501 aggttccgga ccggagcgta tttcacctgc ccgggaggat ttgccgctgc gtcggacgaa
     7561 atgtcctggc cataagccgg aacggaaaaa ataaagggaa taagcaaaag acgggctcgc
     7621 atgggtcaac tcttttctag caggaaaaac ctctgcccta caagcgtttt tcacgtgtct
     7681 gaatgtttaa ttattgatca tgtcacgttc tttttttaaa gaaaaaaatg gagtgggacc
     7741 gggtggttcc gaagacgcgt gcggaagccc gcaaggcgga ggaaaatggc ttcctgggag
     7801 acgggatgaa tgaagtgggc gagggattgg ggattccaac cccgccgtat ttcttcattc
     7861 catcaggtcc ggattctttt gatgaatatc cccggaagat taccttttag taaattccct
     7921 tcaacttttc cggatgatcg ctcaactggg agcgcagctg gtttctgttc agctttccca
     7981 tgatcgtgag ggggatgggc gtttccatga cggcgcggat gcgctggggg cctggggccg
     8041 acgcattcca ctcgaggcag gcctgctcca gaggggcggc ggaagggccg caggcgacca
     8101 gttccattcc ggcgcgcgga tgtggaactg cctccacgac agcctccgga acgctgcggc
     8161 gcaaaacgtc ctgaacggca tccggatcca ccagttcccc caggactttg acaagtctgt
     8221 ccgcccggcg cagaaaggtc agcaaacgcc cttccaggcg caccaggtcg cgggtgctcc
     8281 accactcctg cggagcgact ctttccagaa ggaaccctcc atgagcctgc gtcagcaggc
     8341 ggccgaacaa tttgcctttc ccctggaagc gcaggaggcc gcctttgtcc gtctgagctt
     8401 cccaatgggg cagaagggaa aggcggtccg tatggaagga atctccggga agggccgtgg
     8461 ctaattggga gccggcttcc gtcatgccgt agctctgcac tacgggccag ccaagggtgc
     8521 gtgctttccg tcccgtctcc gtgtccagcg cgccgccgcc tacgataatg cacccggcgg
     8581 tggaaggcgc gcggaggccg aggctgacca gatccactac ctgcgtgggg accagggagg
     8641 accatctggc tccggaactt ttcatcaggt cggcataagc ctgcgggcac cactttccgc
     8701 ggagcgtatg gacggcggtt ccggccaggg ccgcgcgcag gtaaatcatg gcgcccccca
     8761 catgccatac aggcagcggg cagcaccagt ccccgctttc cgcatgtaaa tgttccaatg
     8821 ccccgcgcgc gcatatgtcc agggcgcggc gggtaaacaa aatggctttg ggcggagaac
     8881 cggaagagcc ggaggtggcg aaggctgtga ttccgtccat gccccggagg gaaagaaagg
     8941 cggcggcctc ttccgcccag cccctcaaat ggggcggggc catcacgcag gggccgttgc
     9001 cgcggtccca aggcaggggt gttccggtgg cgggcatggc ggcggcagtt taccggaggg
     9061 cttttctgcg cagggtgtgc cagcgttcct ccatgcgggg cttcaaatga tgctcaatcc
     9121 atgcataggc tgcttccagg ccgcccgctt tgaaagtgcg gcctatctcc gcttccacgc
     9181 ctgtggcgtt gccggtgcgg cgcagaaaat cattgctggt gaattctacg aaaagcttgc
     9241 cgttgatcgc tccttcctcc gccagaatcc attccctgac gtacaacgcc aaaatggcgt
     9301 cccctaccca tgcctctgcg cggaggcgtt cttctgtagc ct
//
LOCUS       DAWEMX010000101         9329 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3077_length_9329_cov_11.437675, whole genome shotgun sequence.
ACCESSION   DAWEMX010000101 DAWEMX010000000
VERSION     DAWEMX010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3077_length_9329_cov_11.437675"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8153..8162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2266 a         2830 c         2246 g         1977 t
ORIGIN      
        1 cccccagctt tcccccccct cccgcgatgc agaggatgaa gatgaagaag acgacgcccc
       61 cccctcgtcg gaaggcagcg gcgccggcca tgtgctgccc ccggacaact tgtatcagga
      121 acccaacgaa gtacctatcc cccatcactc caaaagagaa cgccccgact catgacacag
      181 cgcaaacaca tcctcttcat ctgcaccggc aacacctgcc gcagccccat ggcggaaggc
      241 ctgttccgga aactttccgc gcaccatccg gaatggcagg ccggttccgc aggaacagcc
      301 gcatggcccg ggcaggcagc cagtccggaa acgctccacg tcctccggga gcatggcgtc
      361 aatctctcca gccatgaaag ccgccctgtt acggatgaac tgatgaaaaa cgccacagat
      421 gtctacgcca tgacggaaag ccacctggcc gccctgcttg ccaatttccc cgaacatgcg
      481 gacaaggtca ggctggtcac ctgctacacg ggcaaccgcg gcatcgccga ccccatcggc
      541 tgcggacaga cagcctacaa caatgtagcg cggcaattga caaccgccat ccaggccatc
      601 attgcccgga tggagcaaca ggagtagcaa catctttgac tccttccccc gtcatggctt
      661 ccccatccat acgcattaat ctgccccgcc aggaactggt gctggaaaaa tccggaaaaa
      721 tcctgctcca atgccccgtt tccagcggga aggcaggtac aggccatgaa gaaggttccg
      781 ggaaaacccc cacaggtcat ttccgcatct gcaaaaaaat cggagacgga gaaccggagg
      841 acactatttt catctcacgc cttccggcag gccgctatcc caccgccatc ccgaaaagtc
      901 tgaatgaaca ttccgacttc atcctgaccc gcattctgtg gctggacgga ctggaaccgc
      961 acaacgccaa tacccggagc cgctatattt acatccacgg caccaacgat acggaactgc
     1021 tgggcacccc ggcttcccat ggctgcatac gcctttctcc cagggacatg atggctcttt
     1081 ttgctctggc ggaagaaggg atggacgtgt tcatccagtg ttaagccttt cttttctaga
     1141 cgtaatctaa aaagttatct gcggaaaagg aaaataattg aacaaagggt ctcttgggat
     1201 gtataaagta tgaaggtatc ggtatttggc tgccgtcctc ctgttccata aacatccact
     1261 catccccaat caatcatgga cgatccggtc ttattatccc gtctccaatt cgccatcacg
     1321 atcatgttcc actacatctt cccgccgatg accatcggcc tgggggtggt tctggtcgcc
     1381 ctggaaggcc tgtggctgaa aacgaagaat gacctctacc acaagcaggc caaattctgg
     1441 accagaatct tcggtatcat cttcgccctt ggcgtagcct ccggcatcgt gatggaattc
     1501 cagttcggta ccaactgggc ggattattcc cgctgcgtag gcgacatctt tggcagcccc
     1561 ctggcggcgg agggaatctt cgccttcttc ctggaatcag gcttcctggc catcctgctt
     1621 ttcggctggg acaaggtggg cccaaaaatg cactttcttt cctcctgcat ggtcgctctt
     1681 ggcgcccatt tcagcgccat ctggatcatt gtggccaact cctggatgca gacgcccgcc
     1741 ggatacaagc tggtggaagt aaacgggaaa atccaggccc atataaccag cttctatgac
     1801 gtggtcttca atccgtccac cgtggaccgc ctgacacatg cgctcgccgg ctgctggctg
     1861 gcgggagcca cgctcgtcct cagcgtctcc gcctggtata ttttgaaaaa acgttttacc
     1921 ggaggatcgg aaaaaagctt caaggtggca ttggtcatcg gcctcatcgg cgtggctggc
     1981 atgggcatca cgggagattc cagcgcacga gaagtctccg tccaccagcc tgccaaattc
     2041 gcggctatgg aaggcgtcat ggaatctggc acccctcagg acctccatct catcggctgg
     2101 atgaacccgt ccacccatga agtgacggga atctccatcc cgtacttgct caccctcctg
     2161 acccatcacg acctggacac gtccatcact ggcatgaacg acatcccgca ggacgaacgc
     2221 cctcccatcc tgcccgtctt ttactccttc catctcatga tcctgatcgg ctgcgccctg
     2281 gcggccctgt ttctggcggg cctgtggggg tggaaaagag gctggctctt ccagaagcgc
     2341 tggctgctct ggtgctttgt tttctccgta ctgggcccgc aaatagccaa ccaggcgggc
     2401 tgggccgtag cggaactggg gcgccagcca tggattgtgt acggcatcct gagaacggaa
     2461 cacgccgtat cccccaccct caccgcggcg gaagcccttt cctccctggg tatgttcttc
     2521 gtcatttatg ccctgctgct ggccctcttc ctctatcaaa tcacccataa gatccacaaa
     2581 ggtccggacc aggaagctgg cgaagacgac ggcgccgggg aaggcaaact ccaggttcct
     2641 tttgttaaag actaatcttt atccctcttt aaaaatcatg ttggacaatc tcactctcgc
     2701 agacctgcaa atcatctggt tcatcctcgt cggcgtgctt ttctccggat acgccatcct
     2761 ggacggcttt gacctgggca ccggagctct tcagcttttc atcaagggcg atgaaaacag
     2821 aagactcaca ctaaatgccg tgggccctgt atgggacgga aacgaagtct ggctcatcac
     2881 gggcggagga gccctcttcg ctgcctttcc gtacgtatac gcttccgtct tctccgggtt
     2941 ttacctggcc ttcatgctcc tgctgctcac ccttatcttc cgcgccgtgt ccattgaatt
     3001 cagaagcaaa cagcccatga aatggtggcg caggggatgg gacaccactt tctccatcag
     3061 cagccttctg gcagctctgc tcatcggcgt agccatgggg aacgtcacca aaggcattcc
     3121 tctggacgac catggcaact tcaccggaac atttctcagc ctgctgaatc cttattccat
     3181 tcttctggga ctgacgacgg tcgccctgtt cgccatgcac gggggaatct acctgctgat
     3241 gaaaacccag ggaagcctgc aggaacaaat cagaaaactg ctcagaccct gcgtcatcat
     3301 cttcactgtc ctgatcatcc tgcacggggc ggccaccctg ctgtacgtac cgcacgtagc
     3361 ggccgccctg gaacgctccc cctggattta cggaatcgcc gccctggcgg tcatttccat
     3421 cgcgtccatc tggatattca tccacaggaa ccgtccgggc tgggcctttg tcgcctcctg
     3481 ctccaccatg ggctgcatga tggctctctt cgggacagcg atgttcccca acctgctcta
     3541 ctccatgccc aatccggaac attccctgac gctcgccaac ggctcatcca cccgggaaag
     3601 cctggtcgtg atgacataca tagccattct gggcgtgccg ctcgtcctgg cttattccgc
     3661 tgccatttac tgggtcttcc gcggaaaagt ccagctcaac gaacacagtt actgatgccc
     3721 tgcgggcaaa aatagcgatc agcgaaccga aaacgccctg taccatccgc cctgccgcat
     3781 gaatgcctcc atcttctcca aactcccgcc ggagaaaatt acggcgctcc atcaatcatg
     3841 gaaaaacgat cccctgtccg tggaccctct ctgggccgcc tggttcgacg gctacgaact
     3901 ggggagcggt ggagcccccc gggaaaaaga aaatgcgggg aacgccgcgg acacatcccc
     3961 ctacaccgtt cctccggaaa gcgcggaacg ccgcgggcgc gtcaaccagc tcatccgggc
     4021 ctaccgggtc atgggacacc aatgcgcccg ctttaacccg ctgactcctc cggaccagac
     4081 cggctgcccc gtcaatccgg aagatatggg attccgagag gaggatatgg accagcccgt
     4141 caacatcggc accttcatgg acggaggaac gttcacgctc cgtgaaatca tcaatcggct
     4201 gcaaaaaatc tattgtggag ccatcggatt tgaataccac cacatagaca atcttaaaat
     4261 ccgctcctgg attgaagaaa aaatccagct ccgggcaaac ggcgtggatt atggcccgga
     4321 agtccggaga gaagccttgt tccatctctg caaggcggaa ctgtttgagg aattcctggg
     4381 caagcgcttc atgggggaaa aacgcttttc cctggaagga ggggaaggag ccatcgtcct
     4441 gctggacgcc atgatcaagc gctgccccgc cgccggagtc tctcacattg aaatgggcat
     4501 ggcccaccgc ggaaggctga atgtgctcgc caatattctc cacaaaccgc tgaaaaccat
     4561 cttccgcgaa ttcacgccgg actacctgcc ggaatccccc atcggcagga gcgacgtcaa
     4621 ataccacctc ggttacgccg ccacacgtca tgtggacggt aaagaactcc atatccgcct
     4681 ttcctccaat cccagccatc tggaagccgt gtatcctgtg gtggaaggca gggccagggc
     4741 catgcagcac aacctgcaag acgcggaacg caagcgtgtg ctgccgctcg tcctgcacgg
     4801 ggacgccgct ttctcaggac agggcatcgt ggcggaagtc ctcaatctct ccctgctgaa
     4861 aggttaccgc acgggcggca ccgtgcacct cgtcatcaac aaccagatcg gcttcaccac
     4921 cagcccggac gaagcccgct cctcccgcta cgccacggac gtggcgcaga tgctccaatc
     4981 ccccatcctg cacatcaacg gagaaagccc ggaagacctg gtgtgggcgg cggacttcgc
     5041 gctccagttc cgccaggaat tcggaaggga catcattctg gacatgtact gttaccgccg
     5101 tctgggccac aatgaaacgg accaggccgc cttcaccgcg cccatgcaga ccaagcggat
     5161 tgaggcgcgc cccaccgccg ccgccctgta cggaatggcg ctcagggagc gcggggaatt
     5221 gacggaacag caggagcggg acattcggaa tgacctttgg gagggcatgg aacaggccta
     5281 cctgcaaatg aaggaaaacc ccgcggacta catcctgccc gccaccgcgc aggatgcgga
     5341 tgaaacgccc ctcccgcgca ccagcacgcg gacgggaatc agtccagaac tgttccagcg
     5401 catcggaagt atccttacgg aacttccgga cagcttcacg ccccacccca cgctggaaaa
     5461 acgcttcctg gcccgccgcc gggaagcctt ccgggaaggc ggcctgctgg actgggccat
     5521 ggcggaatcc ctggcatggg gcagcctgct cacggaaaac cataccgtgc gcctgtccgg
     5581 ccaggactgc cagcgcggca ccttctccca gcggcacgcc gtcctgcacg acttcaatga
     5641 cggctccctg tacactcccc tggaaaaact gaatcacggc accaccgcat tccgtattta
     5701 caactcatcc ctgtcggaag cctccgtact gggctttgaa tacggctacg cgctggaaag
     5761 tccggacgcg ctggtcatgt gggaggccca attcggggac ttttccaacg gggcccaggt
     5821 catcgtggac caattcattg ctgccgcaga agccaaatgg aaccagaaga accgcatggt
     5881 gcttctgctg ccccacggtt atgaaggagc gggctcggaa cactccagcg cccgcatgga
     5941 acgctaccta cagctttgtg cggatgacaa catgcaggtc atcaatccca ccactccggc
     6001 ccagtatttc cacgccctgc gccgccaggt gcaccggaac gcgcacaaac ccctgattat
     6061 tttcacgccc aaaagcctgc tctcccgtcc ggaggccgtc tccccgcacc gggaattcct
     6121 ggcctccacc cgtttccgcg aagtgctgcc ggatccggat actcccgcgc cgggccaggt
     6181 cacgcgcgcc gtcttctgca ccgggaaaat ctattatgac ctggctgcct accgcaggga
     6241 acggaatatt tcagacacgg taatcatccg tctggaacaa atctatcctc tggcgcagga
     6301 acagcttacc tatctgctgg cgccctacca aaaagtgcgc gacttcgtct ggtgccagga
     6361 agaaccctcc aatatggggg cctggggcca cctgcggaac aggctgggac gcctcttcgc
     6421 cacttccttc cgctatgcgg ggcgcccctc catggcctgc cctgcggagg gggccaaagc
     6481 gctgcacgcc gccgcacaaa aaagactcat cgccgctgcc ttcgggccgc gggcccaatc
     6541 ctgaacccat tttactccca tgagcgacat cctgactccc aactttggag aatccatcac
     6601 ctccgccacc gtagccgcgt ggcacaagaa tgccggcgac ccggtagcca aaggcgacac
     6661 tctggtgacg ctggaaacgg acaaggtttc tacggatttg gaggcggatg aaagcggcgt
     6721 gctggaaatc ctcgtcccgg aaggggcgga agcccccatc ggcgcggttt taggccgcat
     6781 ttcccccctt gacggaagtt ccgccgcacc gccctccgta ccactggaaa cgagggagaa
     6841 ccccgcttcc ggtccctcct cacctgtgac tggcgtcccg gaacaaaaac cggaaaagaa
     6901 aacatcctca ccggatcaga caacctccgg aaaaaatggg aaaacaggaa aggaggcttc
     6961 tccgcgcttc atcagaaaac ccatgagccc gttgcgccgt accatcgcgg cacggttggt
     7021 agaggcccag caccaggcgg ccatcctgac taccttcaac gaatgcgaca tgtcctccgt
     7081 gatggagctc cgcaaacaat tcaatgcagc gtaccgggaa cggtacggaa ccaaactcgg
     7141 tttcatgagc ttctttatca aggccgtagt caaagcattg caggaagtgc cccaagtcaa
     7201 cgccagaata gacggtacgg acatcgtgga aaacctgtac tatgacatct ccatagccat
     7261 cggcacggat aagggtctcg tcgtccccgt gctgcgggac tgcgaccgga aaacgcttcc
     7321 ggagctggaa ctggaactcg cttccctggc ggaaaaagca cgcaggggaa acctgtccat
     7381 gcaggatctg caaggcggct gcttcaccat ctccaacggc ggaacatatg ggtcgcttct
     7441 ttccacgccc atcctgaacc cgccccagag cggcattctg ggcatgcatg ccatccagga
     7501 acgccccgtg gtccgggacg gccaaatcat cgcccggccc atgatgtacc tggccctttc
     7561 ctacgaccac cgcctggtgg acggcaagca ggccgtgcaa ttcctcattg ccgtaaaaaa
     7621 cgccgtggaa aatccggtgt tcgagttatg aggcgccaca ggaaaaagct aaatactaaa
     7681 gagaaaaaaa caaatatctt tttttgattg gccctgttac gattagtagt tgactgatac
     7741 tttaaccata atgatatatc aagtcataat aaaatgaagg agtcaaaaaa tgaatctgat
     7801 agaactcaag aaagctatga cttcctctca tattcagaca gacaagaatt gcagaaagtc
     7861 cttgaacgct cctatcagaa tcattctata gaatccaagg aagaactgga agaatctaaa
     7921 ttttcccatg ccgtctattg ccatcattgc ggctgtgctg aaaaagtcca gaaatacggt
     7981 aagcaaaagg tatccagaca aaccattgca aagactgtag aaagtgcttc acaactctct
     8041 cctcctccat atttaacaag atacataaaa acttgctgta tggaagaaat atgtagagta
     8101 catgttggag gaactttcca ttcgcaaatc tgcacagaag agtatggtct ttnnnnnnnn
     8161 nnttcttttg tatggcatca caaaatactg gataactctt tcgtaaaata gagaaagaaa
     8221 tttctctgaa gaggttgata gagaagatga aacttttttc acgtgagcta taataatcgc
     8281 aagcataata tgcctcaccc ttcatatcac cgcaaaatag aagcatccaa acgttgtctt
     8341 tcccgtcaac aagttttcgt tccttgtgcc attgatagaa gtaattcctt gctggttaaa
     8401 atctgtaatc ttggcaagat tcctactctt gaattgacca acctatgaag gaaggtaagg
     8461 cggagccggt gctatcttct gtacaataga gagaaatact atcagagagt catatcgtat
     8521 ggttcagaat gaattctgta agcatcagaa gctaacaaca taaggaagct ctaacataaa
     8581 ttcttcacta agttcaaaac aaaatctatt ttgaaacttc attatacccc atccccaact
     8641 aagttactca gcaagatgag gtaagttaaa tatccactta agccaactca gaacggaaca
     8701 agcacgtctc tccattgtag attcactttt acaaacgttc aattcccgca tcttgtcaca
     8761 cacttgttgt ttactaggca attctccgtt gttatctatc acatagtcaa agaaatctgc
     8821 aaatattcta tgttctacca ttaaactaac taacttcaac tggcgttcct tatatgtcat
     8881 acgacataac gacacaccaa tttcagttag cttggtagtt ttattttcta attctctttc
     8941 aaagattcca agatagatgc cagcgcttac ataataattg tattgacgtc ctacaaaatc
     9001 catcaattct attatctgtt cggctgtcat cggattgctt tccatatttt ccaacaagga
     9061 aataagccga tttacagaat cagcctggat aaatggaatg acagtttcac ttatattatc
     9121 actcttagtg acaactgtat tccttctaat atccatcaaa tcttccaaag aaatatgcat
     9181 atcctgtaag gaataattct tagtccgtat caattcaatg gaagaataat cttcaagttt
     9241 attaaatctg tattcaaaca gacgatatat catatttgaa tacacagaaa acactaatcg
     9301 tatcggctta ctaaccttat tcctccata
//
LOCUS       DAWEMX010000102         9273 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3106_length_9273_cov_10.793665, whole genome shotgun sequence.
ACCESSION   DAWEMX010000102 DAWEMX010000000
VERSION     DAWEMX010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3106_length_9273_cov_10.793665"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2141..2150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8924..8933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2123 a         2850 c         2489 g         1791 t
ORIGIN      
        1 atagattttg actttgaccg ccccatccgc ttcgtggaca tggaaggcgg cggctccctg
       61 attacggagc cggaccttat cgcggacgag taccgggcta tcgtggccag ctatctggaa
      121 gaaacgcgcc ggatctgcac ggatatcaat gcggactacc gcctggtcag aaccggagat
      181 tccctggaag acgtgctgac cggcttcctg atgggacgtc agaaaaagaa ggcgaccgga
      241 taaatgcccg gctgtctcac ggaacggttg attgctccgc ggaacggatg caggatgcac
      301 ccagacgctc cccggattgc aggaagcgtc tgtttttcat ctctttccct tttttccatt
      361 gtggatgcac ccgttaaaat gatcgtgggc ctgggcaacc cgggcagaac ctatgaagac
      421 acccgccata acgcggggtt catggtgctt gaccgcctgg ccgcccggtg gggggccgta
      481 ttcaaggcgg acaggcagcg caagtgcgag gtggccgccg gccccggcgt cctgctggtg
      541 aaaccctcca cgttcatgaa tgaatccggt ctgtgcgtgg gccccatgat gcggtttttc
      601 aaactggatc cccgcagcgt gttcgtcatt catgatgaag tggatttccc cctgggcgtg
      661 atcaagctac gggaaaaagg ctccgcaggc ggccacaacg gcattaaatc cctcatcgcc
      721 cacatgggca cccaggaatt cccgcgcctc cgtttcggca tcggccagcc ccgtggcaaa
      781 ggggaaatga tagggcacgt gctgggcaaa ttccgcccgg aggaacgcga gcttctggac
      841 gtgatgcttg gcaaggcgga ggacgccgtg ctgtacacca tggaacacgg cattaccagg
      901 gcaggcaata ttttcaacgc tgttccaggc tgacagccgc atgaaaggcc gccccatttg
      961 cgccatgacg tatccctgga cgggccggag agcgtttttt cctgaagccg gagcagcccg
     1021 ggccgcagga tgatacggct gcctcaggcg cctggcggcg tccaggaata ctcagggggc
     1081 gcgcatgcct tcttttttcg cccgtccgcc atgtacggta ttgctaaaca aacgggaatc
     1141 cgttaggatt tttgcacgtt ttccccactc ctcaggacca atcctgcttc atcttcatgg
     1201 ctgaattaag ttcacaagct ccccgcaggc acatgcaggc caacaaaagg cggcatccca
     1261 aggcaaagcc caggaaacga ttctggagct ttatccgaaa gcttgtcctg tggtgcctgg
     1321 tgatggcggc tatcggcgcg gccgtgggat acttcggatt catgatggcc tggaaacgtt
     1381 atgacaactg ggcggcggaa tttgatctgg aacgcatcaa tgacctggaa aaacccagca
     1441 ttatttatga ccggaacggg gaggaaatag gccgtattta tgtggagaac cgcagctacg
     1501 tcacgctgga caaaatctcc cccgccatga ttaatgcgct gattgcccag gaagattccc
     1561 gcttccggga acatccgggc tatgacttcc tggggattct gcgggcaggc cgggagtata
     1621 tccacaacag cggggacgcc aaccagggcg cctcctccat cacccagcag cttgccagaa
     1681 atgcgtatga cctgaaaaac cgtgccaaag ccaggaatga gggcagcttt ggccgcaaat
     1741 tcgtggaaat agcgctggcg cgccgcatta cggaacggta cagcaaggac caggttctgg
     1801 aattttacct gaaccgcgtt tacttcggca gcgggtttta cggcatccgg gccgcttccc
     1861 tgggctactt cggcaaagag ccggcggatc tgaccacccg ggaggccgcc tccatcgccg
     1921 ccctgatcaa aaacccgaac ggactttccc ctctgaataa tccggctagc aatctgaaat
     1981 ggcgcaacca cgttctcaac cgcatggcca aggagaaata cattacgccg gatgaagcgg
     2041 aacgcctctc cgccatggag ctggggctga atcccaagcc gctgaaacgc ggcgtctccc
     2101 acatttacga ccgcatttcc tcagaaatca ccgcatacct nnnnnnnnnn cgtcaatgcc
     2161 gccgggctta aaatttacac caccattgac aggcagctcc aggaagttac cggaaaagcc
     2221 ctggaacagg ctctggaaaa cattgaaaaa caacccggtt accgtcatca gaaggccgca
     2281 gactaccaca gcggttccgc gaacaagccg cgctatctgg aaggagcgct gctggtggtg
     2341 gacaacaaat caggcgccgt cctggcctac cacggaggca gggattacac caagcgccag
     2401 tatgacgcca tcagggacgg agcccgcccc accggcaccg ccatcctccc tttcctgtac
     2461 gctacagcct ttgacacggg aaaaagccct gtcacccgca ttctggacga tgccatcgac
     2521 aaccgcctga cgggcatcgg cggttcggaa ggaatcctgg gggaatgggg ggcggaaaac
     2581 tccaaaaacc gttatgaagg catgattacc gcccaccgcg gcctgtcctc ctccaaaatc
     2641 gccgcatccc tgcgcatggg gatggagatg ggcacggccc cgttcgtcaa gaagctgacg
     2701 gatttcggca tccgcaagcc cgtgagggaa gcgggcagca cggaagtaaa tcccgtttac
     2761 cgccccaaaa ttttcgtggg cacggaacct gcctccctga aggaaatgac gttggcctac
     2821 accgccatcc cgaacggggg ttcccgccct caggatattt actatctgga caggatagaa
     2881 gatgaagacg gccagctgat ctgggaatcc acccaggcga ttgaaacacg gaacaatgcc
     2941 caggtacgga agggggccac atccacagcc acggctttcc agcttcacaa catcctgaat
     3001 tcctccctga aaaacggttc agcccagcgc gttgccccgc atctgccgaa aaacttcaag
     3061 ggcggcgtga aaacaggcac cacgtacgac ttcgcggaca actggctgtt tgggtatgac
     3121 agccgcatta cctgcggcat ctgggtaggt ttcctggaag gcaaaaagcc catttatcac
     3181 ggagccttct cgtcagacac ctgcgcctcc gtgctgggct ccgccatcac ggaagcggaa
     3241 cggcagttcc ctgccggaga actggcccct ccccccagcg tagagcgggt ggaaatctgc
     3301 ctgaccaccg ggaagagggc cacgcacagc tgttatgaca ttgacccgtc ggataaaaaa
     3361 taccgccgtc acaccatatt cgaatacctc cgcaaggggg attccagcct gcctttctgc
     3421 gacctccacg gggaggacgt ctccgcaccc gtctccacct tcacggccca gaaccgcatt
     3481 cttcccctgc cgcccatcct gcccaccagg cccatccttc agggggacga tccctaccat
     3541 acggagctga accaggtccc ggccaaccgc aattttgaac tgctgggggc tggcgaaaac
     3601 gtgccggaag cgcaagccat ttccgcagat cccgtttctt cagaccatac ggatacaagc
     3661 attacgctgc cggcgcccag gcccatcacc attcccgtgc cggaattcat ccacctgtaa
     3721 ctttttcccc tcatgccttc cccatcccag gacacggtag cctgcacatg cccgtcatgc
     3781 ggcaacctct tcgccgtgaa ggcgtctttt ctgggacgcc aggtaaaatg ccccatctgc
     3841 accgcttcag tcacagccag gcaggaagaa aagcccaggc cggaaacgga agaacaggac
     3901 gccgtcccct gcctctgcca tatttgcggc aattcctttg ccgtcagggc cgcctgcgta
     3961 ggcagccgtg tggaatgccc cgtctgccac tccgctgtca cggccgtcaa acgggaagaa
     4021 gcgctttcaa cattcccggg ccggacgcct gaagctgcag agaaagcgcc cggaaatccg
     4081 aaacaaccgg ctattccgcc atctgccgcc ggtgttccgc acacttccgc gtactccgct
     4141 ccgcatacac cgctcgcacc aaagaaaaag acggacaaac ggaccatttc cgttcacggg
     4201 cctaccactt ccggctccga caaatccacg acaaaaatcc gtaagaggaa agaaggaaac
     4261 gccgctccgg ccattgccgc gccacgctgc gccgccctgg ccccggaaga accggaatac
     4321 cgcgctgccg ccgcggacac catgcgcagc gaacgccggc ccacctggca tatatggctt
     4381 tttgtcgcgg gactggttct ttcccttctg ggcgtgttca tgttcctgcg cgggagggat
     4441 atggaagcgg aaaactccaa acttctgaat atctcagacc ggttcgtgga tcatgaagcg
     4501 ctcttttcac aggaagtgaa taaagacctg ctggagcaat tgcagcagag ggagcggcaa
     4561 taccggctca tagcccacag ccgcagggag gaagaccgga aggcggaatc catttccccc
     4621 catattacgg cggccatgaa tgaactggcc ctctactgca tggcggaatc ggatgaagag
     4681 aggctcaact acgtaatgaa tcccgcagcc gtagctccca aaatggccta ttgggcctcc
     4741 tacggccaat acaaggacta cctgccccag gaagcgggca gaagctccaa aaacggggac
     4801 ctgcttcaga tctccgtgct gatggacgac aacactatcc gccctgcggt tttcctgtat
     4861 gaccggaatt caggaaaatg gaagctggac tgggaagcct gggagggcta ttcccccatg
     4921 cttccggcgg agctggaagc caaaaagccc tccacccccg ttcccgtcag aatcatccta
     4981 agcatgtccg gcatttatca gccccctttc ctggaggaat cctccgcgga aagctaccgc
     5041 aacacgtcat atctcaactt cacccttgag ttccccaacg gggaacgcct gaatgcgtac
     5101 gtggacaggt attccccgct ggccctggaa ctgaccaggc tgctttacaa cggccccgtg
     5161 cgcacctgcg tgctcatccg ctatccggaa gacctgcccg gcaaccgggc cgttattatt
     5221 gaccagcttc tgcactccgg ctggatgagc gacgccaccc gcaggcttct tcccccccaa
     5281 taattgaaaa tcaagaatcg gttcccgctt tccatgagtt ccaaagcact agcgcaaaca
     5341 caggacaaag ccctccaact caacctggct tccaccattt acggcacctt tgcggaaatc
     5401 ggagccggac aggaaacggc caactggttt ttccgggcat ccggagccgc cggaacagtg
     5461 gccaaaacca tttccgccta tgacatgacc gtcagcgaca ccctgtacgg gccggtcaag
     5521 cgctacgttt ctgaagaacg cctgaaaggc atgctggact acgaatttac ccagctcatc
     5581 aaccgcctgg gcccccagcg cggcaaagac acccgcttct ttgccttctg caatacggta
     5641 aaagtcaaag gctaccggga caacggcccc tggcacggct ggataggggt gcgcttccag
     5701 ctgaaaccgg aggcaaaacc gtccgacctg attattcacg ttcgcctgaa cgacccggac
     5761 cacgaccgcc agatgaagga cctgggcatc ctgggcgtca acatcctgca cgccgtcttt
     5821 ttcaaaaggg accgtctgga ggaattcatt gaatccctgg tggacaatct ggatccccgg
     5881 ctggtggaaa tagatgtcgt ccgttttgaa ggccacggtt tttccatggt ggacaaccgt
     5941 cttttcgcgc tccagctcgt ccagtccggc ctgacgccgg ccaccctgtt cctggcgaac
     6001 ggccaggtgg cccaggcggc ggatgtgctg tacaagcgcc ccatcgtgtt gatgaggggc
     6061 agtttcaacc ccgtctgcaa tctgcatctg gaagtcatgg cccaggtgaa aaaaaccttc
     6121 ctctcccaca tcaatgagac gcaggcggac cgctgcatgg aaatttgtga aatatccatg
     6181 aataacctgc tccgcggcga tgatgtggac catctggaat tcctggacag ggcggacagc
     6241 ctcaaggctc tcggcaaaaa cgttctgatt accaaaatgg cccgtttcga caacctgtcc
     6301 gggctgctgg cgcgctacac gagagaaccc atagccatcg ccctctccat cggcctgctg
     6361 aatgagctgt tcaaggaaaa atggacggag gacattcccg gaggcattct ggaatccttc
     6421 ggacgcacct tccagaacaa aacgcgcctg tacgtctccc cgtggctcaa ccgcaaatcc
     6481 ggggaattcg tcacggcacg cactttccgc gcgccggaac aatacgtgca cctgtaccag
     6541 cacttcctgg cgaacggcct ggtggtggac gtgccctttt tcaatgaatt cctgctgcgc
     6601 cacacgccgc gggacatcca gcgcatgatc gctgcggatg aagacatctg gaaaacgctg
     6661 gttccggagg aagcgcaccg ttccgccctc cacttccgct gagttccttt tcagcaggcc
     6721 gccccttcct ccccgcggca cggccctccc aatgccgcgg tctctccgtt cctgaaaccg
     6781 gataatccgg cacggcccct cccgcctgcc ctgccgggct tcccgccact ggtcaggaaa
     6841 acttaacaat tccgtaagat tgtcttgcca taaggcagga atctcattaa ggtctccgag
     6901 ttccattttt atcatgaata ccattattat cggctcccaa tggggcgacg aaggcaaggg
     6961 caaaatgatc gatttcctga cggaatccgc cgacgtggtg gcacgcggcc agggcggcaa
     7021 caacgccggc catactgtaa ttgccaatgg aaagaaatat attctccacc tggttccctc
     7081 cggcatcctg tggccggaca agctttgcgt catcggtaat ggtgtggtgc tggaccccat
     7141 cggcctggtg gaggaaatca atgaactccg cggccagggc gtgaccatta caaaagacaa
     7201 ccttctcatc tccgaccgcg cccacgtggt ccttcccttc cacaaggaaa tggacgccgc
     7261 ccaggaatcc gccctcggca aaaaagccat cggcaccacc aagcgcggca tcggccccac
     7321 ttacgcggac aaggcacgcc gcatcggcgt gcgcatggcg gatatgaggg atcccgcgat
     7381 ttttgacgaa gtgctgcgcc gccgcatcaa gatggcgaat gcggaaatgg aacgcatggg
     7441 ccttcccgct atggatgaag aaaaaatggt ggcggaagta agcgccgccg cggatgtgct
     7501 gcgcccccac atcaccaaca ccattccggt tatgcacgaa gccgtggcct ccggaaaaag
     7561 catcctcttt gaaggggccc agggagctta cctggatgtg gacttcggaa cctatccgtt
     7621 cgtcacctcc tccaacacct cttccgccgg cgcctgcacc ggcacgggtg ttcctccgca
     7681 caaaattgac cgcatcatcg gcgtctgcaa ggcctacacc acccgcgtgg gcgcaggccc
     7741 cttcgtcacg gaggatgaag acatctccaa ccatctgcac agcatgggcc gcgaattcgg
     7801 cgccaccacg ggccgccccc gccgttgcgg ctgggctgac ggcgtgctgc tgcgcttctc
     7861 cgccatgttc aacggttttg atgaaatggc catgaccaac ctggacggct acgacaagtg
     7921 cccggaaatt aaaatctgca cgggttacga tctggacggg gaaatcctgg cttatccgcc
     7981 cgccacagtg gatgaatggg aacgctgcaa gcccgtgtat gaaacggtgc ccggctggat
     8041 gcaggacatt tcctcctgcc gttcctggga agagattccg gaacaggcca agaaattcgt
     8101 caaacgcatg agcgagctca tcggctgccc cgtcacaacc gtgggcgtag gccccgaccg
     8161 cgaacagacg attgccgtgc aataaacctc ggcagttctc gttattattt ccttcaaagc
     8221 agttccgcat gcatggcgga actgcttttt tattgtcagc taataaaggc cgaacacacg
     8281 tttcaacgct ttctccgcca ccgtttctcc acggcagcgg catgggaaaa acttattttc
     8341 caccctgcat ctttgcatga agcgcggaaa tgcctcctct aaaacacatg aaaacggcaa
     8401 ttttgccgct tccaggtcag gaaaaagcaa ataaatatca tccaaaacgg cccggcggaa
     8461 aactggtgaa gctgccgttg gaaagcccat gcctgggagt tcccggaatg atttgacgga
     8521 tacttccctc ctgatgataa taaaagagga ggcgggatct ttcccgcctc ctgtaaaaca
     8581 gcaattggac tccccggctc aggcacgtct gcggcgcatc atgagggcag ccagaccaat
     8641 caggctgagg gatgccgtag ccggttcggg aacggcagcc gccgcaatgg cattaagatt
     8701 ggcttcctgg gaagcaccag cctgcaacgt ggtattatcc acgacaaggt atttgacgta
     8761 atccgtgcca taggtaaatg aagtcagggc tccgaacccg ctcgctttca accctgaatc
     8821 ctgaccacca taagtggtga cggttccttc ggaatcaacc agagtcaggt aagtccaggt
     8881 tccggtgctg gtaatctgat aagtgatggc ggcggattca tagnnnnnnn nnntgttttc
     8941 ggtgaacaat tccttcaatc ccgtattcaa attgagtgaa gaaccgttga cggaagtaat
     9001 cgtgcggttg tagttctctc cttgccagga accataaatg cccgccgtgg gagttcctga
     9061 agaaacgtag ttgaggccca ggccgatcat attattattg gcggaaaaaa tattcgcctt
     9121 gacgttacct gccaggatgg cgtcttggaa agcctgagcg tccagggcca tggtcacgga
     9181 gaaattgccg taaccggagt ttataccttc cgtcagggta tatgacccgc ccgctgcctg
     9241 caatgcactg gaatccaaag taaggacggt tgc
//
LOCUS       DAWEMX010000103         9247 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3113_length_9247_cov_9.048629, whole genome shotgun sequence.
ACCESSION   DAWEMX010000103 DAWEMX010000000
VERSION     DAWEMX010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3113_length_9247_cov_9.048629"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2024 a         2978 c         2372 g         1873 t
ORIGIN      
        1 gtcttcgtag tgctggtagc catgttctgg gtccgcatcc ccttccaggg ctccatctgg
       61 ctgctttccg ccgccctgct gctcttcatc gtcacggcgg cggccatcgg gctgtgcatt
      121 tcctccttcg cccaatccct tcagcaggct attgtgggaa ctttcctgct gctggtgccc
      181 atggtcatgc tctccggctt cgccacgccc atctccagca tgccggaaat cttccaggac
      241 ctcacgctgc tcaaccccat gagatacggc ctggaactca tccagcgcat cttcctggaa
      301 ggagccggat ttctggatct ctggccgctt ttcgcggcca tcatggccgt tgccgtggcg
      361 gccgtcctgg cagccatctt ctcttttcat cataaaatct cctgatcccc ccttccttct
      421 ccccggaata tccggaaacc tccctgcccg taacttcccc gtttcacatt ttcatcatgc
      481 caatcccgtc accggcataa aaatacttgc atccggcccg ttttttctat tctatttctc
      541 tcgttccttg ggaacatggg ctcgtcgttc aatggatagg acatcggttt ccggtaccga
      601 cgatgtaggt tcgattccta ccgggcctac cacttttttt gaaagaaagt ggttactccc
      661 ggcaataaca ggcctttttc ctttcttgca gcgttgcaga acaatagccg gggctaatcc
      721 ggttacggca tcctccgggg aagaataggc ccatgtatgc gcactctccc ggcttctctc
      781 tcccgttccg cccggatatc ttccggaaat acgccgcctt tcagggaaat aataaaaaac
      841 tcccttccgt taatcgcgga agggagctga atggaaacag gaatccaagt ttgccgttac
      901 ttgcggcggc ggcgcatcgc cagaccagcc aggcccagca ggctcaggga agccgctgcg
      961 ggttcgggaa ccacatagga aacgctgtac ccgttctcat tcttggtaac catataagaa
     1021 cctacttcgg cattagtcaa atcgtccaca gcggtgaggg aatcaatgcc tgcaatgccg
     1081 ctgagcattt ccagggaagt gttcgtgctg tcaaaagaca tattgcttcc cagggtaacc
     1141 aggttcctgg tcaggatttg atgcccgtca ctagtaagtc cactggcggt gcctccgtca
     1201 agagtcagcg tagcggtcag gcttgctatc ttggcggtat atgaactacc gttggaggaa
     1261 gtaaggttca tgatgcccgt ggctcccaaa ttggcgtaaa atccttcccc gccctggctc
     1321 ttggtataag ccaggttaaa ggtgccgtag ttgttcaacg tcgtgcgccc accatcattg
     1381 ccgccgttat aactgttgaa ggtcagcgta gatgattgat caatatcaat agaacagccg
     1441 ccctggaatt tttttacgtt ccctacagtc aaatccgcac cgttttgcag ggcaagcttc
     1501 aatgtccagc cttccagctg ggaaatgctg ccgttcgcac cggaaacgga aataagagac
     1561 catgcgttgg aattgggcgt gccgggaccg gttcctgccg acggccagga atctgaaccg
     1621 gtgatggacc agctggattc tgtctgccaa agctcctggg taatgctctc accgccgttc
     1681 catacaaaat cggcatgagc cattcccgcc agagcaatga gcgtaatgag tgtcttcttc
     1741 attatcagag gaatatataa caatagtaat gacggaatca tctaacggaa agatgaccag
     1801 catacttgcc tcacagaggc ttgggcttca ataacggatt ttcaacccgg aatcaatact
     1861 aaatattgtg gaaaaataat ttaagtatca ttgcaaccct gttttcagga tgatttttcc
     1921 tccaaaaaaa ctccctgccg tggcgggcac ttcctgttgt ttttttggaa acaattttga
     1981 cactaatatc acattttaac aaaatgtaat atttttaata taaaaaagtt actttaaaaa
     2041 taaagattaa tttctccatg ccgcaagaca tctgccacat gcaaccctcc atccactacg
     2101 gcacatgaaa aggattgaaa atccgcatgt tgccgccgca gagggaaacc gccgtcaaat
     2161 aaagaacatt ccagaaatta ttcatcgccg gagaaaaaca tctgtcttgc ctaccgccgc
     2221 ttccgccatc aagggcatca cgcaatcccc tgcctgcaac tatccctcaa cggaaggcct
     2281 ggcacttatt cctccacaga acacggcttt gccacgcctt ttcaaaacgc gccagattcc
     2341 ccagcatccc agcagttcac ctctgacggc ggcagccgcc ggaaaagagg catcaccagg
     2401 aattgccgca tctggcgggc cgtatccgga caaaacggaa aattccccgc cataaggatc
     2461 atttctttcc ctgtactccg gccattttcc ggaacctgcc cgcaagcagg aatccggtgg
     2521 ccgcccaaag aaaggaaatg gcggctccgg ccagcatggc ccacggggaa tgggccccca
     2581 acacgttaaa ggagcttttc agccatgcgc tgacggcgtc gccgccacgg taaaggaccg
     2641 tatcaataaa actcttggct ttatattttt cttccgggga caatacggaa ttcagcattt
     2701 cacggccggg acgcaccatt ccgtattccc cgatgcggcg taccgccatc accacggcca
     2761 cgacggcaaa taccggagcg aaagccatcc atacaaaacc cagggaaata agaacgggga
     2821 cagccgccag caacgccgcc aatccgaatt tccgaacaat gcgtcccgcc agaaatacct
     2881 gaagcagcat ggtacacccc tggacaacaa cgtccagcgc tccgaatacc tgcgtctggc
     2941 gcaccggatc aggaaaggaa gacgctacaa cgctcatcag ctcaaaatac aggaacgtat
     3001 tggtcccggc cagcaggatg atgaacagcc cgatgcccat caggaaggga gaccggaata
     3061 cggcggacgc cccggcaaac gggttccctc ccagagggcg ttcccggcaa tcggccggaa
     3121 gaagaactcc cgtttcctca tcttccgggg aattcccgtc acgccagcgg tgcaggtaca
     3181 ttcccgccag catcgccagc gccagaaaaa cagcggcggc aaggaacagc cacagatagc
     3241 cggccactcc ggcaagggcg gccgtcaccg ccggccccgc catggcccca aggctgcttc
     3301 cgctggcaac cagggcaaac aggcgtttca tctgccccgg cttcatgacg tccgacagca
     3361 cgctccacgc cacggaaacg gtaagcaggt taaatacgga cagccacaca taaaacgaac
     3421 gggccgtcca gacatttccc gggcatgcca gcacgagcgc cgcgaaaacc gccaggttaa
     3481 gaatgaaaaa accgtacgtc cacggaagaa tggcttttct ccgcaccttt gaggaaaccc
     3541 atccgaataa aaactggccc cctatggaag cggcgaaagt agccgtaaaa agccactgca
     3601 gattctgaac gcctccggca atgcccatcg tctcccggac cggcctcaac atggaatagg
     3661 ccaggaacaa caggaaaaac aggaggattc cccccgctgc ggcaggcaat tcgcggggtt
     3721 ccgcatgcag caccttttcc aggttccggc acaggcgttt cttcattccg ctggacatgc
     3781 ccggaattta ccatgcccgg aagatttttt cacttaaatc gtcccgttcc ccaaaaatcc
     3841 gcctttcttt cacctccccg ccggaacttc tccttccaac tggacttgca ggataagccg
     3901 gattatgatg gggcatccca ttccctcatg tttcccgccc catcaggaat catgaagcgc
     3961 ccccatgaac cagagaaaaa tcatccatgt ggatatggat gccttttacg catccataga
     4021 acagcgggac catcccgaat accgcggcaa gcccatcgcc gtaggcaggc cggaaatgcg
     4081 cggcgtggtg gcggcggcca gttatgaggc gcgccgtttc ggagtgcgtt ccgccatgcc
     4141 ttccatgaag gctctcaagc tttgccccca tctgattttc acccgcaacc gcatggatgt
     4201 gtacaaggcc gtctccgcgc agatacacgc cattttccac cgttacacag atctggtgga
     4261 acccctttcc ctggatgaag cctttctgga cgtcacggaa aacaagccgg gcattccgct
     4321 ggccgtcgac attgcgagga ggattaagaa ggaaatccgc cgggaacttc acctgacggc
     4381 ctccgccggc gtttcctaca ataaattcct ggcgaaaatc gcttccgatt accgcaagcc
     4441 ggacgggctg ttcacgatcc atccatcccg ggcggaaaaa ttcatcgcgg cacttcccat
     4501 tgaagctttc tggggagtcg ggcacgccac cgccgaacgc atgcgcgccc tttccatcac
     4561 caacggggcg cagctccggg cacgggacaa agacttcctg gtaaggcatt tcggcaaaac
     4621 aggagccatc ttctacaact tcgcccgcgg tgtggacgac cgccctgtgg aaccttcccg
     4681 catgcgcaaa tccgtgggtt gtgaagaaac ctaccgggaa aacgtcacca gggcggaagc
     4741 gctggaacaa cgcctccccc tgctggcgga agaactcgcg gggcggctgg cccgttccgg
     4801 cttccgggga aacaccctta ccctgaaggt taagttcccg gactttgtcc agaagacccg
     4861 ctgcgcgacc gttccggaaa tcctgacgga gaaagaagga attctccccc tggcccgcac
     4921 cctgatggaa gaactggatt ccggggaccg tacattccgc cttctggggc tgtccgtctc
     4981 ccatccccag gaagaacagc ggcagggcat ctgggaacag ctctggctgg agctggagta
     5041 ttaactcttc catagactgc catattttca ggactctccc cttaaaaacg gcaataccca
     5101 caagatatcg tttcaggaat gagcaaaacc cgctccaacc tggtcataaa ccatatgaat
     5161 caggaagaac ggagacactg ggcgtcatgg ataggcatga ttgcggaagg cgtggctgga
     5221 gtcatggccg tgaatggagg gattcccgcc tcccgggacg tacccgatcc cggccctgcc
     5281 cgcacgcgcc gccccctcac ggaaacggaa aaacgcatta tcgaacacca gggaacggaa
     5341 ccgccctttt ccggggaatt cgtcagcttc tttgagccgg gcatctattc ctgccgcaaa
     5401 tgcggtgcgc ctctgttccg gtcggaagac aaattcgatg ccggatgcgg ctggccctgt
     5461 tttgatgatt ccctgccgga cgccgtcaca agcgtccatg cgggcggcgg gcggcgcacg
     5521 gaaatcacct gcgcccgctg cggaggccat ctgggacaca ttttcaatgg agaacgcctg
     5581 actccgaaga atctgcgcta ctgcgtcaat tccctttccc tggaattccg ggcagcccct
     5641 cttccgatag cggcggatgc cccccgttaa aggcgtgcgg caggaaaaaa atatccggcc
     5701 gcccttccat gtgctgccgg atgcggccgc cgcggcggaa tttaccctga aatccgttca
     5761 cggccccgga gcctcagcag gcaacaggga atatccttga cccgcaaaat ccttgccgta
     5821 agcaggatat acctttccgc ccaacctgtt tccctcgtgc cgggaaacag gggggattag
     5881 cttgactttc ccgtaatgtc gcctattctg gttcaagcat tatttgcaac cactcacact
     5941 tattatgacc gaccgcagaa ttgttatcac cggcatcgga gtgatcagcc ccctgggcaa
     6001 tgatctcgca tccacctggg aagccatgaa ggctggccgc agcggcattg acaccatcaa
     6061 atccatggat gttaccggct actccacgaa aatcgccggt gaagtcaagg atttcgatcc
     6121 cgccccgtac ttcaagacgc ccaaggacgc ccgccgcgtg gaccgcttca cgcactttgc
     6181 catgggcgct gccggaatgg ctctcaagga ttccggactg gatctggaag cggaggacaa
     6241 aacccgcatc ggcgtcatgg tgggcagcgg catcggcggc ctcggcacgc tggaaagcca
     6301 gcatgccacg cttctttcca aggggcctgc ccgcgtgtcc cccttcatga tcccgtacat
     6361 gatcagcaac attgcctccg gcctcatctc catggaatac ggcctcggcg gtccgaacat
     6421 gtccatcgtc acggcctgcg ccacctccaa ccacaacatc ggggaagcct ggcgcatcat
     6481 gaaattcggg gatgccgacg tcatggtctg cggcggagcg gaagctacca tcctgcccac
     6541 gggcgtggcc gggttcagca acatgaaagc cctcagctcc cgcaatgacg agccccagcg
     6601 ggcctcccgc ccgtacgacg tggaccgcga cggcttcgtt atgggcgaag gcgccggcgt
     6661 tgtcattctg gaaacgctgg aacacgccca gaagcgcggc gcaaaaattt acggggaact
     6721 cgtcggctac ggcatctccg cggacgccta ccacctcacc gccccggacc cggaaggccg
     6781 cggcgccgcc cgctgcatga agatggctct ggaacacgcg ggaatgaagc cggaagacat
     6841 tgactacgtc aacacgcacg gcacctccac cccgctgggc gacatctgcg aaatcaaggc
     6901 catcaaggcc gttttcggcg accacgctaa aaacgggctg cttatcagct ccaccaaatc
     6961 catgaccggc cacctcctgg gcgccgcggg aggcgtggaa ctggccgcct gcctgatggc
     7021 catgcaggac aacatcatcc cgcccaccat caacgtggac aaccaggatc cggaatgcga
     7081 cctggactgc gtgcccaata aggcccgtga aaccagggtg gacgccgtcc tgagcaattc
     7141 cttcggcttc ggggggcata actcctccgt catcgtgaaa cgattcgcct gaccctttca
     7201 ggcctctcct ttccggcgga agcgctgatg tttgcggcca ccgcagccat cgcgcttccg
     7261 cttttttcat ttaaaagcca tgcccgccaa aacgcatcag gacattctgc tcatgcgctg
     7321 ggccctgttc tccgtagccg ggcttatcct gctcgtggcg ctctattacc tgggccttac
     7381 tcccgtctgg ggccgggagc tgaaggaagc catggcctcc gccccgtttc tgcttccccc
     7441 ggacgccccc gccctttctc ccggcacggg attcctgctg tgcatggcgt tgacgttggc
     7501 cctcgtttac gatttgctgc tcatccacgg cacgctccgc aaaacggccg ttctggccgc
     7561 tttcctcctt cttctggcca gctttacccc ggtgctgggg ctgtggggcc ttttcttcaa
     7621 tgccgtccca tgcctgcttt ccgcagggag cgcgggcgcg ctcgccatcc tctggcccca
     7681 aacgccccct gtccggcgtc attccgaacc tccttccgct catgaataat ctggactatc
     7741 ccatccccgt gcttgaactg gtaaccgccc tgaaacagct tccgggcatc ggcacccgcg
     7801 gcgcggaacg catggccctg tggatgctcc aggggcacat gggagaagcg gaggcgattg
     7861 cccgcaccat cggccaggcg gcggaacacg tcacgccctg ccccgtctgc ggcttcttca
     7921 gcacggaagg ggagccctgc gccgcctgcc gggatgaaga acgggacagc cgcctcatct
     7981 gcgtggtgga acaggccacg gacgtcatcc ccattgaacg cagcagcgcc taccgcggat
     8041 tgtaccactg cctgggcggc aagctttccc ctctggatga tgtggagccg gaagacctca
     8101 acattcaatc cctggtggaa cgcgtctccc ggcagcctga ttgcgaagtc attctggcaa
     8161 caggctctga cgtggaaggg gaggcaacgg ccacctacct ccaccatctg ctcaaagaaa
     8221 tggattgcag gatatcccgc cctgcccagg ggctgcctgc cggctccggc ctcagccacg
     8281 cggacacgct gacgctgatg aaggctctgg aaggacgcac ccggctgtaa aggaaagccc
     8341 ttcccgcatc catcccggca cggggaaatt cccgcccatg cccccgttct tcaccgtccg
     8401 gaatcccggc atggggacgg ttccgtcttt acagcggttt tcctgcaccg ggcaaaacgc
     8461 ctctttcccc caataatgaa gggaaaaacg tccgcctcat tccgccagtc cgtccacacg
     8521 ggcgcggtca aaatccgccg ggcaaatcgt cacgttcacc cggtcgcccg gtttgagcag
     8581 atccctcagg gaagcattct ttttttctac aaaagccacg gcggttttgc cattcggaag
     8641 ggtggcgcgg aaagccgtat ccgcgatacg ctccacgata acggcgaccg cctgcaccat
     8701 ctggcaggga taaggtttca tctgaaaaaa acgggttacc aattcattag ttcgttcatc
     8761 aggtcattcc cggttttaag ccccttttcc gcagcgtcct gaagccgcgg cacaaccggg
     8821 acgggtactt cgtccgcggc gggaatcctg aacttgcggc cattacgttc cgcaccatcc
     8881 cccgctccgt tttccccggc ccccctgttc agcagccgcg ccgcgacatc cgccacctca
     8941 gccgccttgc ccatccctat ttccgtcagg cggtttcccg ccgccttgac gagccgcgcg
     9001 gacaagtcct ccgacgggtt gtccagcgtc ccgccgatat tcacattggc ccacgccaga
     9061 ttgcctcccg ctccctggcg ggaaacggaa aacacatcgg aaaaccccgg aagagctttc
     9121 cacaggccgt cggcccgcag ccccacctgc agccttccgg tcagcgcccc ccctttcccg
     9181 atttccagcc agccttccac cctcaccagg ttttcgcttt ccaccagaat attggaaata
     9241 tgccagg
//
LOCUS       DAWEMX010000104         9084 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3187_length_9084_cov_13.349762, whole genome shotgun sequence.
ACCESSION   DAWEMX010000104 DAWEMX010000000
VERSION     DAWEMX010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3187_length_9084_cov_13.349762"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2220 a         2401 c         2566 g         1897 t
ORIGIN      
        1 catcgcccgc cttgaagccc agggccagct tgacgccgtg ggaggacacg ccggagtcgt
       61 ggaaacggcc acctacggag cccttgcccg gtacaaaacc gccgccgccc tggaaatggt
      121 cacggaagcc gccaaaaaac atgcgctgct cgcgtttgcc acccggatgg cggaagctgc
      181 cggcgatcag ctcaaaagcg cggaagaagc ccttgatgaa gccgaacgcg gcatgtccgc
      241 cctgcgggac cggtgcggcg tccgccagac cgaaaccatc cgcggggccg tgggagccat
      301 cattgaaaac ctgcaatggc gcatgaacaa ccctggagcc atcaaaggga tttcctccgg
      361 ataccgccgc ctggacctga ccctggacgg cctgcagccc ggcgccatga tcgtgcttgc
      421 cgcccggccc ggagtcggga aaaccgccgc cctggtcaat atcctcacca acatctgcct
      481 tgagggaacc cccgtgggca tgttcagctt ggaaatgccg aaatctcagc tcctggaacg
      541 tgtcctctac ggcatggccg gcatcaactc cgacgacatc cgccgcggca agccgatgac
      601 gatcggacag cagcagcatt tcacggccgc cgtcaggaaa atcacggccg ctccgctgca
      661 catcgacgac gaaagctccc tcaccattga cagcatcaga gcccgcagcc gccggatggt
      721 ccgggaacac ggtgtcaaat gcatcggcgt ggactacctg cagctggtgc gctccacgac
      781 ccagcaggcc cggggaagcc gggaacggga agtctcggaa atctccgccg gcctcaaatc
      841 cctggccaag gaactcaata ttcccgtcct ggtgctggcc cagctcaacc gtgacgtgga
      901 aaaaagagcc gggaacgccc agggcaaacc ggtcgtttcc gacctgcgcg attccggctc
      961 cattgagcag gacgccgacc agatcatcat gatccaccgc ccctacatgt acaagcccga
     1021 caagcacgac cccacggaag cgcagtggat catcggcaaa aaccgcttcg gacggctggg
     1081 acacatcccg ttccgctgga cggcagaact cacaaaatac gaagaagagc ctaattaccc
     1141 tgccaataaa tcatgaaaaa actagacatt attactcaac cctgcggatc tcatgccttg
     1201 agaatatccc tttatttggg acccaagcgc aaaagaatga gaatttgtat cggattggaa
     1261 acacacgatt acatggaagc ccagcgccga gcattgcttc ttctccgcta taataaacgc
     1321 cttggaattt atgaccggga aatcccggaa gaatcggaaa ttacatatcc tgaaaaaacg
     1381 gatgacttac ccttgtttcg ggacgacaat gaaattcaaa gcgaaaatgg taactcccgt
     1441 tgatatattc cggcgcaaaa agatagatgc tcgccccatg tccacacgcg aacgggctat
     1501 gctgcccgcc gcagaacggg tgatttctat ttttacggcg gacgttgaaa aagcgcagtt
     1561 tctgcaacgc ctcgccgaca tgcttgacga ctttttatcc ggtaaaaagc aggagatcat
     1621 acttccggac ggtacgtcaa caacggtggg cgtgatgcag gggaaagccg actttatagc
     1681 caaggcccgc gcttttatgg ccgcagaagg aatggcagcg gatgcgggag acaaccgcat
     1741 taccaacatc ggcgcccgtt cccgcctgtc tttgatcttt gacacgatga cccgctgctg
     1801 ttatgggcag gcccgctggg aaagcggcat gaccccggaa atgctctacg cctacccggc
     1861 gtggcgtttc gtccggcatc cgggtgccag gatgccccgt cctttgcatg tactcaatga
     1921 aggagccgtc cggcttaaga cggacttcca attttgggcc gtcgagatga attctccggc
     1981 gataggcggc ttcctgctgc cctggccttt gtacggcttc aactcatgga tggatattga
     2041 aagcgtatct cgagccgagt gtatcagatc tggcctgatt gcccccgact gggttcccgg
     2101 cccggtggac atgtcccgtt tcggggcaac gctgccggag cgtcttatga accggtcggc
     2161 ctccgtccag aaagtgaaag acccggctct tgccgcccgg cttcgggaga gcctcaaaaa
     2221 gcgcctcgga gctgacgcct tggacaggga cggacggctt gccatcccgg cccatgaact
     2281 cgtccagcgt atgcagggcc aggcagggca gggaagcccg gctggaatat caccgttgca
     2341 aatgacgttt gatttgttca acccggaaag aaaggattct gctatggcgg acttgatgaa
     2401 aaaatctggc ttgagaccac gcggggaggc gacactggaa caggtggagg ctttcatgaa
     2461 tgccctgaaa actcgccatc cggagcggga ttgggtcaac gaaaaggtgg agaaaggcat
     2521 tactgataaa ttccgtccta tggcaaagga aactatccat aaaaacatga acgaattcat
     2581 gcggatggta gatcctgaaa ttttggacaa cctgccgtct ctcaattccg ttaataagga
     2641 gttaaatttt ggaaacaggg ggagctataa ccccataacc cggaccattt tttattccag
     2701 cggggaagag ttcgacaaag ataatcattt ccatgaactg gcacattggc tgcattttaa
     2761 tgcagaagat gcaaaaaaga agaagcttgg cgattatttc cagaagagga tcaagggcga
     2821 gaaaaagggg cgccttctgt gcggtactaa tggttattcc gaccattttg caccgtcatt
     2881 tgaacgatgg gatgattatg ccgggaaagt ttatgggcgg gaacccgttg acggcatgcc
     2941 ttacggagtg gagatgccta cacgccacct tcaaaaactg gctttgtctc cagatgagtt
     3001 ccttcgatat tggaatgata caagagacgg caaacattac tggcgcatgg cgtttttaag
     3061 aagtttaacc ttaatgttca aatgaataaa aaaacactgg agttatatcg ggaatatcag
     3121 tcaggaaagt tagaagttaa cgaactgatt agtcaacttg ctgatttgtg cgaaaaggga
     3181 gaatgcaagg tagaagaact aattgtcgca gaaatgaaag ccggcatttg tattatacca
     3241 actttggctg acggagttga actggaattg tatttccgaa aacatccgga aatgagagga
     3301 aaaatctttt tagaatgaag aaaaaatcca ccattccacc gaagagaaca ggacgcccga
     3361 ccaaatatac ggacgccctg gcggatgaaa tatgcagacg cattgccgaa ggggaaatgt
     3421 tgatgcagat tgtacgggat gagcacatgc cggaacgtaa gacagtttat aactggatga
     3481 atgagcatga cgacttttta cacaactacg cgcgcgcgtg cgagatgtcg gcggatgcct
     3541 tggtggaaaa gggcctggaa atacttgacg gaagcagccc cgattgtgcg cagatggaca
     3601 aaaatagggc cgaataccgt aaatggctgg ccgggaagag aaatgcccgt tacggggaac
     3661 ggcaggcagt ggaactcacc ggggccaatg gggggcctgt ggagatgatc acggaatgcg
     3721 acgaagacag aatagcttcc gtcatggaca gaattgaatc catccgcaga aagagggcgg
     3781 aagaagagaa tggcggaacg gtgtgatgac atagtatccc ggtgccgttt acggctggct
     3841 gaattcgccg ttgccgtgct ggggctggac ccctacgact ggcagatcaa cacctatgag
     3901 gacattaacg attaccggcg cacggccgtt gtagcggcta acggttcagg caaaactgtt
     3961 tccctggtag gtcctgttgt actgtggtgg ctgtattgct ttccacgcgg acgtgttgtt
     4021 cttacgtccg gttcctggcg gcagttaaaa acccagctct ggcccgcaat ccgtgcttac
     4081 cagtctcatc cggcattccg gggttggaaa tggaaccaga tggaaatttt gactccggaa
     4141 ggaggcttta cctctatatt ttctaccaat gatgaacaga aggcggaagg gtatcacgcg
     4201 acggcggcaa cgcctgtcct ttatatcgtg gatgaagcga aaggcgttca ggacggtatt
     4261 tttgaggcgg cggaccgatg caccgccacc cggtacctgt atctttcttc ccccggttcc
     4321 gctatgggga agcattaccg ctgctttcac gacgaggcca aaaactggag gcgaaccaag
     4381 gtcacgtcat acatgtgtcc ccacatccgt ccggaaaaac gtgcggaaga cctggaaacc
     4441 tacggggaat cccatcccct ctaccgttcc atgcatcttg cggaatggac ggaaggggaa
     4501 gacatgcttg tcattactcc ggaacaactg cggcatgcga tagaccatcc tccggcgttc
     4561 agaccgggcg gacaatgggc cgctttagat tttgccgccg gtcgagatga aaatgccatt
     4621 gctgtacgag aaggaaacct tgtcagactg gaccaggcgt ttagacaatc cagcacggta
     4681 caggcccggc gccggatggc aaaccgcctc aaggaactcg gcattgaagc acataatgcg
     4741 tggggagact cggacggcct gggactgcct atcgtccagc aaatggccga accggttgaa
     4801 agcggcgggg acggctaccg tatcaaagag ttccgaggag gattgcccgg ggaagacccg
     4861 gaacattatc tgaacaccat ttccgaagcg tggatactgg gggctcgcga catcgtcaac
     4921 ggaaagatcc gcattgatga actcgacccg gtcacattcc gccagatgac tacacgccag
     4981 atggaatggg atcagaaggg ccgcctccgc gtcatgtcca aagaagacat gcggggaaag
     5041 ggcttgcatt ccccggaccg ggccgatgtg attttcatgg ctatttgggc cggccgttcc
     5101 tcccgtggca tttggacgga ggaaacggat gtgtacacgc ctccggacac ggaagggtgg
     5161 tattatgact cttgggcgga aggtcctgtc tcttgcgaaa tttgaaactc atatccagtc
     5221 ccggttattt acggatttga ggattgccgc atcatatttt catgaggcaa gccgccaact
     5281 acaacataca cgccacggaa tccctgccgc agtctcttgc gctgcatttt atttctccat
     5341 ccggtgagga tatggacatc agcggcatga cgctacgcgg agcggtagta caggatgggg
     5401 tgatcatgtt ggactgtgcc gttacggggg cgagtgcggc attggtgaca tggccgaggc
     5461 tggccgccgg atgcggcgct tatgatattt ttctgaccga cgcatcgggc aaagaatacc
     5521 ccttgttgaa gggagccgtg catgtcgcgc ctcgtattac gcctccggat ggctcggaaa
     5581 aggccgcggc tgtgtccgga gctcttgatg tctccatctc cgaaacggaa gacggctccg
     5641 tgaccattgt ggaaaaccca tccattgtgg tcgaggaact tgtacgacag gccgaagcgg
     5701 cccgggatga agcaaagcgg cttgtggaaa cgctggaagg ccaggtggaa agcggggaat
     5761 tggtcaatga ggctgtaacg aataaattgc cggatgcgct caaggaggcg ggcgtggaat
     5821 tggaagcggc aaccgggcaa tccaccttat ccagcggaaa cgtcgccgac acctggacca
     5881 tcgtcggagg ctacgcgatg acgtggggag acgagatact ggccgggcat ttgcccgaca
     5941 gctgccgcct gacgagtatt tcaaccgtgt attttttcac cgaccccgcc ctgaatcagt
     6001 attgcctgcg gatttggcgg ctgacggacg gagcttacag cctgattggc acctccgcct
     6061 atgtgtccaa cctgtccagc ggccagacgg ccacgtgggt atttacgccg ggcgttatat
     6121 tgcagcgcgg agacaaaatc atcatccagg tgtgcgaggg gacggagatg acaccctacg
     6181 cgctgggcat gcacgctgtc ctgacccctt ccgtccctgg gcgcggtctg gtgacggagg
     6241 tggccaaccc gcccacggtg aatggcacga tggccccgct gatgaccgtg gtggtggact
     6301 atgacgacgg catcaccctg ggaggaatgg agctggctac cgcgcgacaa ctggacagcc
     6361 tggggcggga tgtgcgccaa tcttccgcga ccgccgaggc tgcggcgcgg acggctggcc
     6421 agtccgccgc tgccgcgtcc acggctgcca ataatgccgc aacctccgcc accaatgcgg
     6481 ccaactctgc aacagcggcg gctaatgctc tggcggccat gccgcaggtg gacgcatccg
     6541 gcaacatgac gctggccgga ggtctgacgg cggcgggggc cgtcaacgcc aacggaggca
     6601 tcaatattcc gcttgccgtg ggggcgccaa cggacacggc ggcggttaat cgttttttgg
     6661 ctatggggct ggcaggagcc gtgcaggcgt tgattcagcc tctatatctt aaaaccagct
     6721 ccatgcccgt agtgggaagc ggcagcacct ctgttcaata tgccggactt tatgcaacca
     6781 gttcaacgtc ggcagcttcc ggttctcctg cacacagtac gaccacgttt acctttgaag
     6841 ggccgcaggg tcagcataat tacagttctt tcgcgggatt ttcgataccg ctgtccggtt
     6901 caacagcgtc caaatttacc tttggattag gccgtggatc aaaaacggta agaggcggcc
     6961 tgacgatgga ttcattttcc atgattccgg gcaataatct ggccgtcaat tacggagaga
     7021 ttatcgacat cacaaccaca gcggttcgtg attctgtccg gggaggttac gtgcttagag
     7081 tgcgggagat ttattacgtc tcttccggtg gttcatggca ggtgaaaact acggaaagtt
     7141 ttattcccgc aactcaaaac cacccatttc ccgcctgcct caacaggctc attttcatgc
     7201 aggaggggct ttcaagcatg tcgtcatacg aggggaaagc gtcactttat attgagctgg
     7261 ggggaggtca gacaaacacc ttgttcaaaa tagccgccct cagaggtgtt tcaggattcg
     7321 aagacggcat ggggttcagc acgttggtgg cggatgtaga aaatcccaat tcctggacat
     7381 cctcggttcg cacgggagca ggcaatcgct acctttatgc caatggattg atcaatccaa
     7441 tgtacgccgc attggaagca atggccgtca atgccattga aaccgaggaa accgctgatt
     7501 ttgaagatat taacatacct ctctaatcat gaataacgca gagatacaga ttcagtttcc
     7561 gcagcctggt aactggcagg aattcaccct gacggccatt tacccggacg aggaagggta
     7621 cacccgcata gaccgctata aacagaatga tgtaccagag gagcagaccc cggccatggc
     7681 cgccgtagtt gctgcgctgg tgggattggg tgaggattgg caggccgtcc aggtgtgggc
     7741 aacacaggat tggtattacc cggatcctgc caatgaggat gaccccattg tgggagtgga
     7801 ggcggtgtat ctggccgtgg aggccgttaa tccccacggg gggcgcagga tttttacgga
     7861 ccgcgattac ccggagttca tcattaccgc ccccgccgcc gtggactttt tcaaacactt
     7921 cactaccccc aatcaataac aaccataatc atatgactac tagtaataaa tgcaatcacg
     7981 ccttggaaat tgccgaggat atgtataacg cctatgccga cggcaaggac cgctacaagc
     8041 accagccgat ggtgaaatac gccgacctgc cggacgacga taaagccggc tggctgaacg
     8101 tagcagaaca ggccctcccc atcatcggca agcatgctat ggaagatgtt aaaaactacc
     8161 tctccgggca ggccaagacc tccaccggct tgcggaagtg gctctacttg gccggggtgg
     8221 gcattgtcgg tgctgtcctt ggcggcctgg gaatgtccct ctccggctgc ggccattccg
     8281 tggacgtgac gccgggccgc acggaggtat gcaaggacgg ctcctgcctc gtcattgagc
     8341 aggggcatat ttcctattcc caggcccagc cggaaacgga cgttccgccc gtcgtgcaat
     8401 ccctgaaaaa gtaagatcat gtgccagccg atagattggg ttctgcgtgt ggtcaatact
     8461 gtccgcgcag ttttggacgg caagaacggt atcttgacgc tactgattgt cgttctcatg
     8521 agtgggtttg tcggcatggc acttatttac aacgatttca agagctttct ggcagagcag
     8581 accaaaaact acgctattca gacggaggtc ttgaggacaa tagacctccg tctttccaac
     8641 ctcgaacaac aaactcataa ataatatgtt tcacaaatat catgtaagct ttttattcga
     8701 ttctggagaa cacatcaaag agcttatttc ctggctcgct aatgctcctg atactattgc
     8761 tctatctgaa aaacacacaa acatttttga aagaaacccc aatcaaggac gctctttttc
     8821 agatgctctc cacgctttga aaagtggtaa gggagctaga ctggaggggt gggccccgga
     8881 tgttgtgatc cgcgctcagt tcccggacga gcacagcaag atgaatgctc cctacctgta
     8941 tatggaatcc cctttcgggc gggtgccgtg gaaggaaacg ttcatcgaat tgttttctga
     9001 ggagtgggag attgtcgatt aaactgtttc cattttggat acagttctaa ccacttctaa
     9061 ccgaaataac ttataactat gaac
//
LOCUS       DAWEMX010000105         9083 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3188_length_9083_cov_9.597475, whole genome shotgun sequence.
ACCESSION   DAWEMX010000105 DAWEMX010000000
VERSION     DAWEMX010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_9083_cov_9.597475"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2878..2887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6340..6363
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1963 a         2646 c         2439 g         2001 t
ORIGIN      
        1 tccgtcaaaa aaatccggga ttcccgccag cagattatgt taaagatatg taacatattc
       61 attatcaata caattattct gttaaaaatt gaataatatg tgaataaaat cgaagcggat
      121 tgcgaatccg cattttcgcc gcccattacc agataaaata aaaagggcct gcacaataag
      181 ttgtgcaggc ccatccttaa aaaccagaga gaaacgtcca gttcaacctc tggtccattt
      241 ttccaaaaat tccccggcgt ccttcccttc tccaatgtgc ctgagcagag cgctcccaag
      301 cacggcggca tccggccggg catcttcagg cagcgtctcc agctggtccg gcgtctgaag
      361 tccgaagccc agcgccagcg gcacatcaaa aacggaccgg gcacggcgca tggtttccgt
      421 aacgctccgc tccaggctgg ctttcccacc ggtggttccc agcacggaga ccacataaac
      481 gaagccggaa gtataaggct tgtattcccg catgcgttcc acggaagtat tgggagccac
      541 cagagtgacg agcgtcaggc catggggact gaaagcctcc cggaacattc cggattcctc
      601 caacggcata tccggtacaa tgaaaccgct gacgcccgcc tcctccgcat ccaaggcaag
      661 ctgttccaag ccgtactggt aaaagggatt tacgtatccc atcagcacca gttcggctga
      721 aaaaccgccc ttgcgggctt tcagaccgtc cagaatccac ttcaggctga cgccgcgggc
      781 cagggcatcc cgggatgcct gttcaataac cgggccgtcc gctacgggat ccgagaaggg
      841 aactcctatc tcaataatat ccgccccggc ttcgtcaata cgggacaagt gcgtccagaa
      901 ggattccttg tccgggaatc cggcggtaat gaaagggata acggcacgcc tgccgagggc
      961 atgggccctc tccacggctt tctgcaacgg tgatttctgc atattattca tggaagaatg
     1021 cggcttaaag attcaaatat tttttaacga ttcccaggtc cttgtctccg cggccggaga
     1081 gattgaccag gacgtccccc ccctgcggca gttcctccgc atggtccagc acccaggcca
     1141 cggcatggga ggattccagc gcaggaatga ttccttcctc tctggtgagc gtctggaaag
     1201 cgtttaaagc agaggcgtcg caaatcacac cgtaatggac acggcccgtt tccgccagat
     1261 aggcatgttc cgggcctacc ccgggatagt ccagcccggc ggaaatggaa tgggacggct
     1321 gtatttgccc gttctcatcc tgtaacagca tggtcacatg accgtgcagc acgccggggc
     1381 tgcccagatt gatgggagcg gagttataac agccgggctc accggtgcct cccgcctcaa
     1441 cccctaccag acggacgcct tcatcctcca caaaggggtg aagcatgccg atggcattgg
     1501 agccgccgcc cacgcatgcc accaccacat ccggcagcct gcctgcacgc tccagcatct
     1561 gggcgcgggc ctcccgccca atgacggatt gaagctgcct gaccaatgtg gggaacggat
     1621 gggggcccgc cgccgtaccg aaacaataca gggtatccgc ctgatgggaa atccagtagc
     1681 ggagagcctc gttaatggca tccttcaacg tgcgggaacc gctctcaatc ggaaaaaccg
     1741 tcgctcccag caatttcatg cgcataacgt tcatggactg gcgttctaca tcctctgcgc
     1801 ccataaagat ggtacagtcc attttaagcc gggcggccgc cgtagccgta gccacgccgt
     1861 gctgccccgc cccggtttcc gcaataagat gcgttttccc catcattttg gccagaaggg
     1921 cctggccgag ggcgttgttg attttgtggg ctccggtgtg cagcaggtcc tcccttttca
     1981 gccacaggcg gaagcccagt ttttccgaaa ggtgaggaca gaacgtgagg ggagtttcac
     2041 ggcccgcgta attgaccagc agatcctgaa gcgcatcccg gtactcgcgg gacggcatga
     2101 tggagtccat ggcttcctcc agctccatga gcggggggcg cagcgcttcc ggtacgaagc
     2161 ggccgccgaa attgccgaaa tatccttctt tcttcatgcg atgtggtaag gagtgtaagg
     2221 ggtgagtggt ttgagggccg ccagcagttt ttgcgggctt ttctggccgg ggatagcttc
     2281 cacgccggag ttcaggtcta tgccgtccgg agagcattgt tccagcgccc tgttcagtga
     2341 ggcggaggaa aggccgcccg ccaggaacca gggccggggg gaactcagag acgccaggga
     2401 ggtccagtca agggaaacgc cgtggccgcc gccctggctg ccggcatcca gcagaaacca
     2461 ggcgcagctg tccgcccata gctccatttc cctttgcagg gcatctacgg attcataccg
     2521 gtccggccac aatacgcgga tcactttttc cggaccgatg cggcgcgcgc agtccagaga
     2581 atgggcgcca tgaagctggg cgaattccag acgggccgtt ttcatgatat ccatgatttc
     2641 ctccgcgttc tggttcacga aaacgcccac gcggcggatc agggcggagt ccagggctgc
     2701 cgcgcgcgac ggcgcaatat aacgcggact tccgggatga aagatgaagc cgcagaaatg
     2761 ggcccccatc cccatggcgg ttgataaatc gctctggcgg gtaattccgc acaccttgat
     2821 tgcaaatgaa tgcttcttca tatcgtttct tctaaaattg ccgccagttt ttcacccnnn
     2881 nnnnnnngta cggcctgaaa ccccgcttcc gcggcttccc tgagatgagc cccggcgctc
     2941 atggcgctgg cggcgatcca cacctccccc tcacggcgga atttcgccat gtccaggcag
     3001 gcctgcctgt ccgttttcaa tgtgtccagg tcacgggcgt ttacctggat gatgcgcgcg
     3061 ccggagtccc tggcaatttc cagatccgca gcgtcgaaca cttccacaac ggcgtgcatg
     3121 ccgtaagcct ccgcctgctc cctgagaaca cggagcgtcc gtgcatccgg cgtcagacgg
     3181 acaatgagca gcaaagcgga agccggcgta gaagccgtct ccatcacctg aagctgatga
     3241 aaaatgaaat ccttgcggag aagcggaagc ccggcgcggc tcatgcgttc caaatatccg
     3301 atgcggccgc cgaaaaactg ttcttccgtc aacacggaga tgcaggaggc gcccgccgca
     3361 gcgtattgtt cagccacttc ctccggtttc agcccggtgg cgatgacacc gcgcgacgga
     3421 gaggactgct taaactccgc aatgaccgcc acgggctgcc cgtccggcgg ttccgcaatg
     3481 gctttcagga aatccggccg gcgccccttc cacgggcgcg gcagttcccg gcgggaggcc
     3541 atgtccgtga gcagggctat ttcctccgcc ttggcttccc ggaaccggtc aagcagcatg
     3601 aagcgttctc cttcctatgc cggcgcagac ggcttcccgt gctttcgcca tgcagacgga
     3661 caggtccatg tgctcctcca gcaggaacat ggctaccccc acattcagaa tcaccatgtc
     3721 catcatggca cgcgggcctt tccccgccag gatgtttttc agcacgtcca cggcttccga
     3781 cttggaatga acggccagtt cctccggatt gcagggctgg atgccgtaat ccaggggatc
     3841 cagggacatg ggcgtcagcc tgccgttgtg gatgatcatc atcttggtgg accccagcgg
     3901 cgtcacctcg tcatatcccc cggcgccata tactacggca gccttgcgga tgtgggactg
     3961 gcggagcgtc tccgccagca gctcgacgag ttccggacgg gccacgccca gcaggatgtg
     4021 cgtggggcgc gccgggttga tgagggggcc cagcagattg aacagggtgc ggatgcccag
     4081 ctcccggcgg atggggccta cattgcggaa ggaaggatga aaattaggcg cgaacaggaa
     4141 agcgaagttc cgctcgtcca acaggcgccg gacatcttcc ggagctacgt ccagcgggaa
     4201 tcccaggcct tccagagcgt ccgcagaacc gcaggaggag gacacggcgc ggttgccgtg
     4261 cttcaccacc ctgtatccca ttccggccag cgtcagagcc gtggctgtgg agcaattgaa
     4321 agagttgcgg ccgtcccccc ccgtgcctac cacgtcaatg cagtcgcctt ccaaaccttc
     4381 cacccggttg gcgcggccca gggcaatccc cacggcgtgg gccatttcca gcggggtttc
     4441 ccctttcatg cggagcccca tcaaaaagca gcccgcctgg gccggagtca tgcggccatc
     4501 catgatgtcc gcaaagcctg cggcagccat atccgccgtc aggtcctgtc cggcggccag
     4561 ggcgtccaga atgcggctga tgcgcttttc cttctgcccc gaaggcacga cgttatccgg
     4621 gaaattgccc agaagctgaa gcccgttcgg cgtcaggatg gattccggat gaaactggat
     4681 gcccacccac ggacggtcat tgtagcgcag ggccatgact tccccttccg gcccgcggga
     4741 agtaacggta aagcggggat tcggctcgtc ctccttggac tggaccacca gggactggtg
     4801 gcggcccacg gtcatgggat tgggaagccc ggaaaacagc ccagcgccgt catggatgat
     4861 gtcggaactc ttgccgtgca tgacatacgg agcgcgggaa acctccgccc cggcgaagta
     4921 gcccagaagc tggtgcccca ggcacacgcc cagcacggga acggaagcgg ggaggcgttt
     4981 caggaactcc aggcacagcc cggcgttgcg cggatggctg gggccggggg aaatgcacac
     5041 ggcttccagc tccgggctgg tcgccagttc cagaatacga ggatcgtcat tcgcatgcac
     5101 cacgggcttg cgtcccaagg cataaaacgc ctgcaccaga ttccacgaaa acgaatcgta
     5161 attatcaatg aacagaaaca tggtcttctc ctttcaaaat aacgtcaata atcttaccct
     5221 tgttcatgca ttcctgccat tccgcggccg gatcggaatc atatacgatg ccggctcccg
     5281 tctgccagtg gatgcgccca tccttcaccc acatgctgcg gatggtgatg ccggaatcca
     5341 tgtggacgcc gtttttgtcc aatcccagcc atccgatgca tccggcgtac ggtccgcgcg
     5401 gcagaggctc ttcctccgcc aggatttcca tggcgcgcac cttgggggct ccgctaaccg
     5461 tcccggccgg gaaggcagcg ccaagaatgt ccaagacgtc caacccgtcg ttcacctggg
     5521 cggtcacgcg ggaagtcata tgcatcacat gggaaaaacg ttcaatctcc atcaggcgtt
     5581 ccagtttcac ggaaccgggc ttggcgacac ggcccaggtc gttgcggccc agatccacca
     5641 gcatgacatg ttccgaacat tccttcggat ctttcaggag atcggcagcc agagctgcat
     5701 cctcctcgtc atccctgccg cgcctgcgcg ttccggcaat gggggaaagc tgaagcttgc
     5761 cgtctgtgca gcgcaccatc agttccggag aagatccgaa cagcgtcacc tccggcaggc
     5821 gcatgaaaaa catgtatggg gaaggattaa tgctgcgcat gcggcggtac agcatgaaag
     5881 cgtctccgtg gaactccgcg gaagcctggg aggaaagcac cacctggatg gcttctccgt
     5941 catgcagcag ctccttgata tgctctaccc ccttcatgta ggctgcctgg tccggtgtgc
     6001 gggaaacttc cccgacggaa ggttcctccg ccccatggag cggggcatgg gacaaatccc
     6061 ggtgttcacc caggctgagt tgggtaaggc gattgtacaa atggtcaaaa acaatcacgg
     6121 tgccggccag caccaggcag gactctgcgg aagaagccgg aatggcctgc gccagcctgg
     6181 gctggaacag ggcggcggtt tcatacccga aatagccgta caaagcccgc gtaatgggag
     6241 cctgcctcat atcgtccccc accagctcca gacgttgcat cagagagcgc agtccatcca
     6301 ggtaaggcat gccttccagc tccttcaggg aagccagacn nnnnnnnnnn nnnnnnnnnn
     6361 nnngaaaggg aaagcctggc gtctacacag gaaacggtca tcagataatc gcaggcgatg
     6421 atgctgtagc gcccccatct gccgtccact tccgcgcttt ccagtagaag tccgggaatt
     6481 ttgttctgag tcaggctcag aaacaggctg ataggggtct ccagatcggc gctcaggcgg
     6541 cggctgcgct gttggagagt gatcatggat ggtgtttgca tggatgatga tgtaagttga
     6601 aaataaaaaa actgctttga ctctcggagg aatcaaagca gttcttgaaa aatggtcata
     6661 gctgaatgct attactgcac ctcgggattc ccccgtccgg acatgggtac gcgccaccaa
     6721 tagccgttgc cataataacg gttcttgcta aaaaagggct gtacagtgct gaaatgcatc
     6781 tgcgcgttta ttactcctct gccccttctt tgtcaaaaaa actttccgtc aatggcgaaa
     6841 aaaccgtatt ggcttctcaa tctgcccaga gaaaacacga cgcctctccg gcgcttccaa
     6901 acttccaaag gaaccgcccg ccccactccc accggcggaa cggagctgcc aactggcgca
     6961 gcacgtttct cttttccttt tttacgaatc cagaattccc ttgccagaaa ccggttattg
     7021 caagccttgc catcggactc gagccttctt cactcccccg gtttccggca tatcttttca
     7081 caaaaagaaa agggagaaag agcgtcttga acattcgggg ctggactcca tcacttcttt
     7141 ttcctgtaga gcttgtcatg tttctgacga tgtttccctt tataaaggaa atccgtcttt
     7201 tcgtccctgc ccacggtgcg gtcaatcaac ccggggatgt cttcttttga atatccggct
     7261 tccagaataa aattctcaac cttgccgtat tgaccgggga attcggaaac aagcagtttt
     7321 atcaaggctt tttcaagatg cttgacttcg tctccaaata cttcatttct cgtctcgata
     7381 aaagaacggt ggcttgaacc ttcacgcatc agtttgagga cttcctgaac ggggagacgt
     7441 ttgattcttt ctatctcacg cttccggaat tccttttcct tgtcggaaag gggagggacg
     7501 ggatttctct gggtatccct ctgataggga ttgggctcct gcgcgttttt gtactgtttc
     7561 aggatgcgcg cgtaataggg aagaaacagg cgccggatga tctgattttt aacgggttcc
     7621 ttgattttgt tcgtcttccg gaccaggccg atatattttt ccatgaggag gcagactctg
     7681 gatttgtggt gaatatcagc cctatcatcg cccatatcaa catccggctc gatttcccgg
     7741 tacgttttgt aggtaaaaag gggagccgag gctaccagat aacaatgcca tattttctct
     7801 ttcagctcgg tatcaatgtt atcctgtgtc gtcaaactaa aggtgagcgt atgtttcttg
     7861 gccccgttat agaaggattt gagccgcatg tccttcagca aaccggagag ttcccgaatg
     7921 gttgcggacg gctgtttgta ctggtcgatg ctcgccgggc tctccagggg agaatcggaa
     7981 taggcctgat tgcccttccc gatcatcagg cagacggcac atgcgcataa aggaggtaac
     8041 aggttcattg ttttttaatc ctcattgctg gttcttgtaa aggtgatgcc gaatttctca
     8101 acaaccgttt ttgtggtatt ttgatcaatg gcttcaatac ggtatttttg atttgtcgtt
     8161 cctaaaaaaa tggaatcctg ttgttcactg cctttcccta cctttcccca atgaatatgg
     8221 aagttgcata caaaccaaaa ttgttgagga ttatgtttgg accggcgaat cgtttcaaga
     8281 acttcctcaa tattgcgatt gtcccctacc atatcataaa aattattttt atcctgaatg
     8341 ggagcggcca tgttgcaggt tacatgctcg gctgccagtt ccggttgagg ccgcggggga
     8401 gtcccgttct ttccttccca catgagtcct ccatcccgct caatcacctt gacaccctta
     8461 aaactgacgt cagtaggaaa aagcgtcact ttaacaaatc cccagacacc gtgttccccc
     8521 ttggatattt taagatcggc cgtattggcg gaaaagccat ctcccagatc catgtgtccc
     8581 tcatattttt ccttcgttat cttcttggga atcctgatat tgacggtgat tttagcttct
     8641 ttgccttcac cggttttggc tgtaatggtg acgcgggtat ccttggttaa atcttttctg
     8701 gcggtcaggg cgatcttttg ttcaccttta aatgactcat tatctgtcgg ttgaaatccg
     8761 tctcccttga tactccaagt aagttccttg atatctcccc tgggcttgcc gatcagaaga
     8821 aaggagatat tctccccgac tcctatattt tttctttttc tatcgtcttc cttgtcctgt
     8881 tcacttcctc ccttgatata ataatccgca gagcacaact ctgtctgaga tttaatctgg
     8941 agggggccgc aggcttcctc ttccgtctcc ttcatgtcat catccggacc atccgaagcc
     9001 cacaagggaa caactccgga aaaaactgcc agacagcaga ataaaaaccg gcaggaagaa
     9061 aaaggtagct ggcctattct gtc
//
LOCUS       DAWEMX010000106         9032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3208_length_9032_cov_15.385318, whole genome shotgun sequence.
ACCESSION   DAWEMX010000106 DAWEMX010000000
VERSION     DAWEMX010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3208_length_9032_cov_15.385318"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6211..6220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1480 a         2813 c         2730 g         1999 t
ORIGIN      
        1 gggtagtgcg gttcctgatc cagcccatct ggtcgcgcag ggcgtccttc tgggccgttc
       61 ccagcgcgtt gaccgtgctg cctgccgcgg ctgccatgga acgtgccgcc cgggaggtgt
      121 tgccggactg gatgtcgttt ctgacggctt ccctcaggtc accgagctgc gtgcctttcg
      181 gcgcgttgcc gcggttgttc cagagcaggt tggcccccgc ttccgagttg gccgtgttgg
      241 ccgcgtccat aaaggcgttc tccgtcttga ccatgcccgt caccacaatc ttgccgcctt
      301 ccgtccggat gccgaattcc tggtagtaga agtcaaagta gccggaggcg tccaccgtgg
      361 ctccgttggc cgaaaccgtt ccggctcccg tcgcgtccac cagcgtgaag ttgaggttgg
      421 ccgtcgggtc cgccgggtcg tcgtagtccc ccaggcttcc cagggaaacg gtggttccgt
      481 ccagattgac gtctcctgcc tgcacggtga tgaccgggct ggagaagagg tcgtcacggt
      541 tggtgttgat gaccaggtcc agatgggagt tttgcaggtt gagtccgttg gcgacggtga
      601 gcgtcgtgtt gaccgctccg gttccttccc ctaccgggct caggcgcaca tgggcgccgc
      661 ttcccgccgt gaggctctta taggtaacgg agccgttgcc gccttccgca cccgtcagtt
      721 ccagcgttcc cttcgtgacg ctgagggcct gcccgatcgc gccgttcccc ttcagaacct
      781 gcgtgccggt gccgttcatg gtgatgctgc cttctccgct gagagagcct tcatacaggg
      841 cattcccggc agtggtgatt tccagggcgg agccgttgtt cagcgcgccg cttcccgtca
      901 ggccggaaat gctgttggcc gcgttgccga gctccagcat tccgttgccg tccaggtcca
      961 cctgcacgcc gtcaagcttg gaggtgccgt tgagcgccag ggaggcgttg tccgccacct
     1021 ggagcacgcc ggctccggaa acggtgctgt tggaaagttc ggaaccggct cccgtgagcg
     1081 agagggtacc gtttttcccg atggagaggt tgccgccgtt gccttcatcc gtcagcgttc
     1141 ccgcttccgc cttcccggta atgtccaggg aggaaaggtc gccggagttg gaggagttga
     1201 gcttgatgga gccgaggtcc gccgtgccgg agaggatgag ttggccgtcc tgcatgtcca
     1261 gcgtgccgct ggtatccagg gttccggcaa gctccaggga gccgtctccc gtcttggtga
     1321 tattggccgt ccccccattg atggttccgg agtacttcgt atcgggaccg gacgtattcg
     1381 gatcggttcc cagcagctcg ttgttcagga tgacggaagc cgtcttgtcg ttcgtgctgg
     1441 tgatgttgat aacgccggaa gccgcaccgg aaagattgtt gatcgtcagg ccgtcagccg
     1501 tatcgtccac gccggggagg ttgagggcca ggttgccgtc cacgatgacc gcgcggtacg
     1561 ggtccagctc ggaattgttc gtgaccgtct tgtcttcgct tcccacggcg taaatctgcg
     1621 tggcgtcccc cgtgatgccg atggagcctc cgttgatgcc cgtcaccgta aagcccaggg
     1681 attccagcag cgggttgaag atggccgtac cgtagcccag cgctccgttg gtcaggttga
     1741 gccacgccgt gcggtccccc gcgccccagc cctgcatggc gttaatcacg tcagcggaga
     1801 gggtaagggt gagcgtttca atttccaatt gcgttccggc cgcgtcaaac tgaatcatgg
     1861 aagtgcttcc ggccaggtcc tgcgtcttgg agacgttccc cgtgcccacc accaggctgg
     1921 cgctgtccag attggcggcg ccgttgatgc cgctgatgac cgttccggcg tccgtcatgt
     1981 tgacggtctt gagcgcggaa ccggaaaggc cgcccaggga aatggcgctg acgccggagg
     2041 ccgcattgac ggagacgttc ttgacgccct tgtaattctg gccgcccgtc acgttgcctc
     2101 cggtgatgtt gagcccggct ttatagccat cggcggtgtc ctggaaggaa agcgttccgg
     2161 aagcaagcgt cacggtaccc gtgtaacccg cactggcggc ccctatgctc agagtgcccg
     2221 taccggtttt cagaaggttg cctgaaccgg tgaaggcgcc gttgagggtg atgtcccccc
     2281 ccgtggtatc caccgtggcg gtcttgccgg aagcggcgtt gagggcgact ccgacagaag
     2341 ttgtccaggc agcggaggag ttgagcgtgc cttctcccag cgtgagggtt ccggacgtgc
     2401 cggtgatatc gccgcccatc gtgagcgtac cgccgccgct gagggtgatg ccggagtcgg
     2461 agccatccag gaaggaggcg gccgccagca cggcagtgcc gccgttgatg ttgagattgc
     2521 ctgccagggc tccctgcccg ttggcgccgc ccaaattgac gccggtggtg gaggtgagcg
     2581 tgacgcccgt ccccacgttg accgcggaag cctgcgtgtt gctgcccatc gtgccgccgc
     2641 ctatttgcag cgtggcgata tcaaggtctt tcttcaagtc cagcgtcacg ccgtacgtga
     2701 cgtcaatgag atcggcattc agcgtgccgt tcatcgtgat ggtacggttg gccgtagccg
     2761 ctccgtagcc ggaaagaacg atgctgcctc cggcattgct gagcgtgccg ttaagcgcgt
     2821 cattgccgtt ggccgccttg acgagcaggg agcctccgtc cgtcttggcc agggtgccgc
     2881 tgatgttctt gagcacgccg gaaagggcga tttcattgcc gccggagagc ttgagggtgg
     2941 agccgctggc caccgtcacg gttccggaga ggaccttggt attggcgatg ttccccagcg
     3001 ccacggctcc attcccggag atgttccagt tgagggacag gaagctgtcc tgcccggccg
     3061 tattcaccgt cagtgtggtg ccggctccgg aggcggccag cttgaccgcg tccccggcgt
     3121 tcttctgcac catggtctgc gtcagcgtca cgttcccgga ggcctgcagg atgccgccgc
     3181 cgaagatgat ggaacccgtt cccagcgctc cgtctccgtt gatgtccaca atgccccctt
     3241 ccagggacgt agaaccaccg taggtgttcg tattgcggat ttgcagggtg ccgtcccccc
     3301 tcttggtcag gatggtcgtg gcgcccgtga tgtgcccgtc tccctggaag gtgtaggtgt
     3361 tgtccgcgcc gctgttgctg acgacgatgg aggagggagc cacgtctccc gtgatcgtca
     3421 cggttttgtt ggctgccgtg ttgtcaaaga gcaggggctg gccgtccgtg gtcgccggcg
     3481 cggagccgct tccgccgtag gtccagttgc tcacgtcgcc cgcgctccat gttccggcgt
     3541 ccgccgttcc gttccaggtg agcatgttcg cggaggattc gatcttgagc tgaagcgttt
     3601 tgccgtccgc ggaaagtccc agcgtgtagg tcagttccgg atgggcgtca ttgatgaggt
     3661 ccccgatgtt gaaggagccg agcgtgagcg ttccgcttcc caccgtcagc agggaatagt
     3721 ctcccgagtc ggtgagcgtg tcgtagtttt tgagggtgac gttgtgcgtt ccggtgagcg
     3781 tcagtccgcc ggtgagcgtc accagggcgt ttcctcccgc cgtcagtcct gtcatgtcaa
     3841 aggtgaagga ggagcccgcg gccatggcca tgtctccggt gacgttcagg cgcgagtagc
     3901 tgcccagcgt caggctgccg atggagccgc cgttgctggt gatggcgccc gtggagagcg
     3961 catccgccgt gccggctttc acgttgaccg cgttattcac cgcataattg ccgatgtcca
     4021 gcgtcgctcc ggaggagatg gagaccgtgc tggtcccgaa cgccgtagcg cttcccgccg
     4081 ccaacgttcc ggcttccgca ttggtcgtgc cggcataggt attggcgccg ctgagcgtca
     4141 ccttgcccgg acccttctgg gtgatggaac ccgttccgcc gatgacgttg gccaccgtct
     4201 ggtccgcatc caccgcatag gtcaggaaag cgtctccatt gccgccgagc gtcaccgctc
     4261 cggaacccag ggagccggct cccgtcagca ggatggtccc ttccttgatc agggtctggt
     4321 tggtataatc gctggccttg gtcagttgaa gcacgccgtt cccctgcttt acgaggttgg
     4381 cgccgttgcc ttccttggct acgattacat ccagcttcaa atctgccgta ttagtatcgt
     4441 ccagcgcaaa attcccgcgc gctatgtcaa agatggccgt gccgccgcgg agggtcaggg
     4501 cattcgcctg accggtggag gggactccct ccgccacgga gaagacgctc attccggtag
     4561 cggactgctg cgtcacaatg gtattggtgc cgctgaacaa gtcgaagccg tttaatcccc
     4621 cgccgggcat gttcagaatg acccggctgt tgttgaacac cattctggta ttggccatgg
     4681 tcgtattgcc gccgccgaca ttccaggtgc cgctattcag ggtcaacgtg ccgaaattcc
     4741 cattgttgtt gtaaccgaac ggatcgcccg cggtagtgtt cagcgtgccg ccgttcacta
     4801 tcaaatcgcc gtaaaggatg gattgctgtc cgtctccatt cttgccgagg ttcaggaccg
     4861 cgccctcgcc caccgtggtc agcgcggttt tcgtctgggc ggtggaaacc gtcagggccg
     4921 ttccggcgcc cgacacttcc agattgatgc cggagccggt cagcagtttg ccgagcgtat
     4981 tggcccctcc ggaggcaaag gcgatcgtgc ctccgttgat cgtcggcatc gtggctccca
     5041 gaatatccgc aattccgccc gttacgccga tttccagccg tcccgtcctg tccacggtca
     5101 ccgttccggt ccccagggcc atgttcgcct tcacggcaac ggttccttcc ttgatgttga
     5161 ccgcgccgga ataatcgttg ttggagctgt tgaaggtcac ttttccttca cccagcttgg
     5221 tcatggcgcc gtttccggca agcttgcctc cgttgttggt cagctccagg tcctgcccta
     5281 tcccgttgtt gaaataaatg gccttggcat tcacgtcccc cgtcagggac gccactcccg
     5341 tgtgcgttcc gtcaaacaga taggtcgtcg tgttgtccac ggcgccgatc ggattgccgt
     5401 cccagaccac cacgccgtcc gccatgctga cggaaacggt caccgtcttg tcaccggtat
     5461 tcacgttgaa ggttccctgg tacaggctgg ccagcgtacc ggtcagggtc atcgtccccg
     5521 catctgcgaa gttgcctgcc gtcaggttgt cccaggaaac cagcgtataa gtccccgcgg
     5581 tgatattccc gttaatgtcc aggctgacgc ctgcatccgc gcccagcgcc aggttgctca
     5641 ccgtcagcgt cgctccgtcc gagctcaggg cgcttccgtc aaccgccagt gaagcggcgt
     5701 tcagcgttcc ggtcacgttc tgcttccgca gcgccgtcac cttggcggtg gaggcgttca
     5761 gcgttccggc cagcggcgta tccgcattca ggtaaaggtt ggctccggaa aggttcagcg
     5821 ttccggaaaa gccggagaca tccccgccca gcgtcagggc tcccgcattg ttgcgggtat
     5881 tgactgccag cgttcccgtt ccgcccaggc cgccgagcat cttgaggctg tccccgctcg
     5941 cgatggtcgc cgttcccgtg ccggacgttt ccacggatac cttgccgtta agcgtcgcgg
     6001 ttcctgtgat tccggtaaag acgttcagga cggtcgcggc gttcgtccct ttcagcgtga
     6061 catccccgtc cagggacact ccccccgccg cggctgcggc attcaagccg ttgaacgtca
     6121 ccgtgacatt tttttcactg ccgcccagcg tgacgctgcc ctgcccccac ggggtcagca
     6181 cctgcccggc agcgacggag gctgtgccga nnnnnnnnnn ggctgatatt accaccggtt
     6241 ccggctattt ccgtatcgcc gtcataggaa ttcttcaccc atcccgtttt aaaattccag
     6301 ccgttgcccc caacatcgga aatgattttc aggccgcccg ttcccgtgat ggcggaggtc
     6361 agcgtggcat tattcccatt ggcggccccg tcaatgatgg cgtagccgcc ttccccggca
     6421 tccagccgga tttgcatgtt gatggagcgg tcggcccagg tgttcccgtt ccacaggcgc
     6481 agcttggaac cgttccccat cgtcagcgtg cccgtgcccg ttattgaggt aaaagcgtgg
     6541 ctcactttca taacggcgtt ttctcccagg gtgatattca ggttggcgct cagcacattg
     6601 ctcgtcgccc cgtcggtgag caagtcaagc gttcccgatt ctatgaccag cgtgccgtca
     6661 cccgatgcgt tgaacgcgtc cctgctcagg gacagcgtgc cgtcacccgt cttgacgacc
     6721 gtcccggaaa tgcctcctcc cagcgtggcg ttgcccgctc cgatcaccag cgagccggct
     6781 ccgctgttaa tcagcttgcc tccgtaggcg cccgccgcca gattcaggct gccttctccc
     6841 gtgcgggtga tggttcccat ccagccggcc agggatgccc atgtgacggc attcccgttc
     6901 gtatccacca gaacagcgtt ggaagccaac gcgcctgtct gaatttgtcc ggaaatgtcc
     6961 gccgttatcc cagcgccgta tttcagagag cctccattaa agatgatgct gccggcaccc
     7021 agcgcggcgt catgccgcat ttccaccgtg ccgccgccca gcaggacttc tccggaaaaa
     7081 ccggcgttgt ccgtggcgat ggacaaggtt ccgctgccgg tcttctgcag cttggagctt
     7141 cccaccaggg aaccggaccc cgtccacgta taaccggttg cgtcagcatt gacgacgatc
     7201 aggcccggat tcacctgccc ggcgatgttc acggtctgcg gcgcattgcc tgctacgtca
     7261 ccgaagatga ctccgttgcc gttggcaaaa ggcaatcccg cattccaggg ggaatccccc
     7321 gttcctccca ccgtccacgt attgtctccg ccgcccgtcc aggtcaggga cgtgacctgc
     7381 gccacgacga atttcagggt attgtccgtc gtgctccagt tgccgatgta ttcggtcccc
     7441 agcagaatgg tattggccag atccccggtg atgccgcccg tggcattaat cagattgtac
     7501 gtgcccgcgc tccagctcac gctgtcgctg ttctccagca gcaggctgcc gttcaccgtc
     7561 agggcgccgt tcagggccag caggggattg ccgttcaccg tcatgtgagt ggcgtcaatc
     7621 cgcacggccg ctcccaggtt caggttgccg gtcatcgtat tctgcctccc aagggacagg
     7681 acgccggcgg tcatggacag gccggcattg ctgtccacgg cggagacgtc caggcccgct
     7741 cccgcggaca gggaaaggcc gccgtacccc gcggaaatgc cgccgatggc cgcattcccc
     7801 gttacggaaa ggttcttgtt cgttcccatt ccggaggcat ggctgacaat gagggtgccc
     7861 cctttcagtt ccacgccgcc cgtgaaggaa gaggaattgg ccagggtcag cgttccggag
     7921 ccgtccttga tcaaacgccc ggttccacgg aatgcattgt cactgcccgt gatcatgttc
     7981 cctgccccgg taatggtcag ggcgccgttc agctggatat tatgagtgtc gtcagcagtg
     8041 atggacatgg tgctgccggc attcaccacc tccacggcgc cgccgcccgc ggcgactgtc
     8101 aggccgttcc cgaaggttcc gtcgctttcc ttggtaatct ccaagcgccc gttgttcaag
     8161 gtccagtacc ccgtattgac ggccatggaa cccagccccg cgccgtcata ggtatattct
     8221 cccacgcgca gcacgccgcc gttggtgacg gttaccttgt agcgcgtata attacccccc
     8281 gtcatgctga ggctggctcc cggagagctc aaatccagcg tggcgttatt aatagtcagt
     8341 gttttggaga ttggatcact cccgattttc tgcgtagccg ccgttttaat cctgaaaacg
     8401 ccgccgccgg tgatggtgag gctgttattg ccaatattga acgcaccctc cgcctgggta
     8461 agcgtctttc cggcctcaat ggtcagcgtc aggtcgttgg cgccgatggt cgtagcctca
     8521 ttccaggtaa cgtccgtatc aaacgtctgg ttggtcgtaa tggtgagggg atcagcctga
     8581 acagaggcgg acaacgcagc gcagcaggca agaagagcgg acaacaagtt gacaggcaga
     8641 tgaagcttca taaaaaataa ctaatagtca atatgttcaa cacgacgtaa ttttttcaac
     8701 agagataaaa tacgctatta accatatgca atatcggatt cgaaatccgt tgtcaatgac
     8761 catttttgag gccaagccct ttaggaagca tggaacggcc tgaaagggga aagagaaaaa
     8821 aacggggata ccagtttcaa gaatcggcat tattaatatt ttaatcatta tctggattca
     8881 tttatatcat ttacggattt cgaatccgtc cggttattta acctgtttct cccagcaaga
     8941 aaaccccgca tgcaggaaaa aatgatttct tgtcttgcat ttctgcggga gcgcctgtat
     9001 aagccttccc gtccccgccg caaggcaggg ac
//
LOCUS       DAWEMX010000107         9012 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3219_length_9012_cov_11.470805, whole genome shotgun sequence.
ACCESSION   DAWEMX010000107 DAWEMX010000000
VERSION     DAWEMX010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_9012_cov_11.470805"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1561..1570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4531..4540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7810..7819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1423 a         2503 c         2894 g         2162 t
ORIGIN      
        1 cagtgtttcc ggagtgattc cgcagaatcc ttcccgttcc gtccaccagg cgtagggata
       61 atgggacgga gagctgctga tggcccgcag gatgagttcc cggggggtgt gggggagcag
      121 catttccccg aactggggga tcgccggaac gcttttcatc aattgcccgg cagcgatttg
      181 ctccatcatt tccgcaaaaa cctgcgcgta tgcgtccgga gacggttccg cccaccggat
      241 ttccggagca ggcggggggg gatttccctc cgccgggaga gcatgaaggg ctgccggtat
      301 tttccatggc cgcgggtcgt ccagccggaa tgtatcaata taaaaggcgg ggccggaatc
      361 cggcggttcc gctgattccc ggaaaggccc ggttccgaga agaagaccgt gggaaggatg
      421 cttgatcagg gctccggaag tcagcgtttt tatgtccatg cgcaattcat gttaaaatgc
      481 cgccgccggg aattccagct atttgaatgt ttgtgaagtg cggaagaaac gggaatgacg
      541 gttcaatggg cgcctccgtt tttccagaat gtcccatgga tggcatggac ctgaaaaagg
      601 gcattcccct tcgttttcct cctccgttga aaaagcaggg gatgacggcc ttcccctgcc
      661 cggagcctgt aacggagaga aactccgccc ctgtttcctg gagccgtgcg ccatgtcacg
      721 tgcgttcccc ttttgaattg acgggaaaaa gcactcaagt aagctttaaa gagcttccaa
      781 ccctatgcag ataatgaaca gaccagcacc atcagtcccc accgccgata ttccggtaga
      841 ggccatgctg gcagaggtgc ggaagccgct gggacccgtt tggtggagcg tgtttctcgc
      901 cagcgcattg ctggcggcgt ggggcatagg gtggagttcc tggcgcattg ccgcagaggg
      961 tgtgggcgtg ctgggggtga ataataacgt ggtgtggggg ctggacatcg tgcattttgt
     1021 tttctggata ggcctggggc atgcgggcac cctgatttcc gccgtcctgc tgctgacgcg
     1081 ccagtcatgg agaagcccga ttgcgcgcgg agcggaacag atgaccctgt gcgcggttat
     1141 ttgcgccgcc gttttccctg tggtgcacgt agggcgtgtc tggatggcgt ggatggcttc
     1201 cccgttgccg gaagtgagcg gaatttggcc ggacatggcc tctcctttga tatgggatgt
     1261 catggcagtc agcacctatt ttcttctttc cctgttgtac tggtatatcg gcctggtgcc
     1321 ggatttcgcg ttgctgaggg attgctgcaa ggggcgtctg aggcgccggt acgggtggct
     1381 ggcgctgggc tggcagggaa cggggcgcca gtggcgcgcc tatgaaaagg ccagccttct
     1441 ttttgcggtt attctgactc ctctcgtggt atccgttcat tccgtggtga gtttcgattt
     1501 ttccgtgact caggtgccgg ggtggcacca gagcattttc ccgccctatt tcgtaggggg
     1561 nnnnnnnnnn ctcagcggca tggcgatggt tcagctgatt ctgctggggg tgaggcgtct
     1621 gatggccggc agcggcgttc gccgggccgt tactccggcc attctggatt taagttcccg
     1681 gtttgtgctg gccctgagcc tggtgatggg agccatgtat ttgtgggagc atctggcagc
     1741 cattctgaat ggaggctctc cggagccgtt tccgggcaga aatcctgtga atgccgtgtt
     1801 cctcatcgtc atggttgccg ggaatgtggc gctgccccag ttgttctggt tccgctctct
     1861 gcgaaccaac cgctgggtga tcgccgccgt ggctctggga gtgcttgcgg gcatgtggat
     1921 ggaacgtttc tggattgtcg tgaattccct gaaggcatcc ctgctggctg ccaacatcgg
     1981 agagtatttc cccagcgtga cggatttggc catgatggcc gggagcgtgg ggctgtttat
     2041 ggccctgtac atggcgctgg tgcgtgtggc tccgttcttc tccctgtgcg acgtgcggga
     2101 acagcaatcc ctgaacaagg agggcggggc atgaaaaagc ccgtcatttc cggctgggtg
     2161 ctttccttcg gttcggagaa ggcgctcctt caggccgtcc gcgtgctggt gaaggaggaa
     2221 aatctgcgct gggaggtttg cgcaccttac ccctgcgccg ctgttcgtct tgccaacagg
     2281 cacgccggga aggctgtggg agcaggcgtc cggctgtggg ccgcagcggg cggcgtctgc
     2341 gggtttctgg ccgtggctct gtggctttac tggacgcagt tctgtgcgga tccccttgtg
     2401 acccagggca gagtccaggg ctgggacagc tggcccgcat atgtccctcc cctgtttgaa
     2461 ggaactttgc tgggggccgg attgctgacc gccgccggtt tcctgaaggg ggctcttctc
     2521 cctcagtggc acgactgggc gttcgagtgt gattttttca ggacggatga gcacgggaac
     2581 ggttacttca ttctgctgga aggcgggagc gaaggccatg ccgccgtttt ggctgaagcc
     2641 ctgcatccgg atgcccgcga gtacgttcat gggaaaggag gccgggcatg agaggaggca
     2701 tcacggcggt agcagccctt gttctcctgg gagcgctctg gttcctggca ggggacgacg
     2761 atggaagaaa tgccgttccc cgtttgtttg acaatatgaa tgatcgtcct ctggcggatg
     2821 cccagcaggt ggggatttct tccgccgccc cggaccggaa aacgcgtttg gcgcctcccc
     2881 gctccgtggc gcagtccctg ggcatgaacg gaacggtccg aaaacgggag gatgaccggg
     2941 actcctttgc tgcggaaggt tcctattttt cctccggccg catgcagggc ggagaggagg
     3001 attccatgcc tctggaactg ggcggcatct cccgcagccg cgacgtgctg gcggaggggc
     3061 gcgcgcttta tctggcgcat tgcgccgttt gccacggaga ggacggaagc ggacgcggaa
     3121 gcatggccgc ttatgacacc tatccgcaaa taggcccctt ccgggatgaa aaatatgctt
     3181 cctattcttc cggaaaaatg ttcaggagca ttcggttggg gcagggaaac atgcccgctt
     3241 tcggaaacat cctcacggcg cgggaaatct ggtgtctggt ggcgtttatc cgccatttgc
     3301 aagcgccgcc ggaagaacct gccgttcaac agaaggaaca acattcatga acagcaatac
     3361 atcttcaaca ggcggggaac gccaggaccg tccgggattc ggctgggccg ggcgttccgc
     3421 tgccctttcc ctgtggatca tttccgttct attatggtgt gtgctgatgc gccagtcctt
     3481 tacggccatg ccctgggacg acatgacggt agccggagaa aggctgcagg aattgagaag
     3541 gccgttcgcc gctgccggaa tggccgtagg agtccttggc tccagcctct gtctggggtg
     3601 cctgttctgg ctctgcctgt cttcactgac gggttccggc tggggcattg cgctgagaag
     3661 ggtgctggcg gcgggttgct gggggttttt acccggtgtt ttggtattgg ccggaacggt
     3721 cggctgcctg actaccgtga tttttccgga agcagcccgc tactggggag tggcggatgt
     3781 tgcctctccc ggcgtccagc ccttcggcgt tctggatttg gctgacttct catggtggaa
     3841 cccggcatgg ctgtacgtgc gcgtggggag tattgcggtg ctttcctgga gcatggccca
     3901 ggtatgggaa acgctggttg cggaaaaaaa tgtctggcag ccttccttcc gccggggctc
     3961 tgccgccttc tgcatggttg tgacggtgat gatgctgggc ctgctgggga tggacatgct
     4021 gggagggacg ggaaaggtcg ttctttccat gttccccgta tacatgattg catatgttct
     4081 gctggcgtcc ctggcattcg ctgtgttgtc tgccgtgttc ctgaggcggc tggacggagg
     4141 gaaggagctg ccttggcccc gcctgggcgg catgctgacg gccatggttc tggtgaaagc
     4201 ctatattgtg tattctcagt acatgattac ctggtacgcc tccattccgg ctgaaatgtc
     4261 cttttataac gcagccgctt cctccggctg gtccggaatg tttgccgcgg ctcttgttct
     4321 tcagcttctg ctgcctttcc ttattttgct gttccccgcg ttgagacgcc ggccgtccgc
     4381 tttgggcgct gtatgcgccg gggtgctgtt tggctccctg ctggaagcat gctggatgtt
     4441 tggccccgga cttgggctgg atccgtccgc atggaaggca tggcttccgc ttgtcctgct
     4501 cttgggaggg atggccctga tctgctgttt nnnnnnnnnn gcagggtatt tcccgaatca
     4561 tgatgaaccg taccgccgcc gttttttttc tgtttttccg ccgtccgggc gagtatctcc
     4621 ccctgttctg gctggcaggg gctttcgtgc tgggtttcct gtggtcgctg gcgtacgctt
     4681 ccggtctggt ggcggaggag cctttgccgt ggccggccgg ccgcctcatg ttcgcattct
     4741 acggcatgct ggtgtacggc tgggccctcc ccatcctgtt ttcctttgcg gcgggaagca
     4801 ggagccggtg gatgaaaccc gcagaatggc tctggcatgc ctgcgttgcg ctggggttgg
     4861 cgactattgc cgcggggaat gggagcggcg tgctcctgat gccttttgac tggtgggttt
     4921 gcccggtttt tgccttcctt gccgcagggg cggcgtgcgt cgcatggttc cgtccgtttc
     4981 cgtggcctgt acggctgttg ttcagttcct ccgccgggtg cgtggctgcg gccctttggg
     5041 cgttgggata tacgcaggca ttgtccatga atggcctgct ggatgtttcc gccctgggag
     5101 gaagcctgtc ctgcgggctg atgtttgccg ccctgggagc ggcggcgctc cgcctggata
     5161 tggcgcaggg gagggcagcc ggaattctgt ccgtctggat ggccctggtg ctggctgcgg
     5221 ctccggtgat tggcgggctg ggggggcttc aggggtttcc cgtgccgtgg atgttggtgg
     5281 aggccggtac cgcctggttt tggtgcgccg ttcttcccgc cgtcctggcg gtggcgcgtt
     5341 tttggataaa atccggtacg aagaccggaa tatgggtgaa gaccggagtt tccctgcttg
     5401 ccttgctggc ggcatggaac gtatttgttg tgggtgcgcc tgagctgatg cagttttccc
     5461 tgtctgcgtg gcatgaaccg gaattatgga ttctgctggg ggccggagtt ttgatgatgg
     5521 caggggggaa ggcccccgga catttccctg cggcatggcg gctgttgggc gcaggcgtct
     5581 gcggcgtgct tctggcgtat gccgtgggcg tgcttgccgc cctggatgtc caggcttttc
     5641 ctgaggcccg tcgcgcggag ctgatgagcg gctgggtggg catggctgcc tgcatccacg
     5701 ccgtagccat ggctttttgc ctggcgggca gcctctgcct gtggcggcca gcgggagcgg
     5761 aagacggagg ccgggagtct tcccctccgt ttttgcggcg tttcttcttc atttcagccg
     5821 tagcggcggc tttggcgctg gcgacggctg cggccgttgt tctgcatgct ccgcctccgg
     5881 ataccatgga ggtcaggagg cacgttcatg atgcggaagg ttccggtatt tatgccgcgg
     5941 aggggtgtgc cctgtgccat acccagatta tccggcgttc cctgtccgga aaggactggc
     6001 agacggcgat tgaccgcggc acggatcccg atttccccta ccgggtcagt gaaccggaag
     6061 acatggatgc cgaattcaac agggaagggg ctccgcaggc gggtgtggcg gccataggcc
     6121 cggacctgag caacgcggcg gaatacgccg ccgggaggct ggaatatgaa gacgccgtat
     6181 ccggcggcac gcgccgcgcc gcgcaggtgc gggagtggct ggccctgcat ctttacaacc
     6241 cgcgggaaat gcagttcaac aaaccctgga ccatgtgtcc ggccatgccc ggactgtttg
     6301 aggaacgccc ggtggaaggg aacgccccct ccacggctgc cctgcctgtc cggatggaac
     6361 ggggccggga gctggtgcct tccccgcgcg gggagcgcct gttgaactac ctggagtccc
     6421 tgcggagggt ggaaccctcc ctgaagcgcg accaaatcca ttccttcccg gccctgtccc
     6481 acattcaccc ggattatgcc gcgcatcctc ccgccgtgga catggaacgc ctgaagaagg
     6541 cccgcgccgc cgccgtgatg gaaaagggca ggggcgtgta cctttccaaa tgcgccatct
     6601 gccacggcaa cgacggcatg ggggacaagg tgacctatcc gcccctggcc gggtccgagt
     6661 ggctgaagga aaagcctgat gcggaaattg tcaggatcat cctccaggga ctgaccggac
     6721 ccatcatggt gaatggaaag gaatgggatt ccaccatgct gccgcccggc gtcacggact
     6781 cccgcgacct ggcgaacctg ctcacattcc tgcgccgcca gttcggcggg gtggagaagg
     6841 cggcctatac gccggagcag gtggacgcca tccgccggga gctgtgacgg gagggccccc
     6901 gattacaacg tttcatgctc cttgccccgg gaacggaaat ggctgcaaat gccattctat
     6961 gaatttagat gatacgtccg ccctttctgc ggacagaaac gttgtaatcg gaacaaatgc
     7021 cagtgccgcc aatggcacgg acaatgttgt gataggttac ctcttccacc agagacgacc
     7081 ggggtaccgt tgtgggttcc acgtccgtgg gcaccaactc cgccggaacc gccctggggg
     7141 atcaatatta aagtagggaa ttcctgcgtt tccgtaggtt cctggtccaa tacggaccac
     7201 tgttacatgg ggatagccct gggttatcag agtaatacgt ccaatgaatc ggagaagctc
     7261 catacggcca cggcctattc cttctgcgcg ggcatgggtt cccggatcaa gggggccttc
     7321 tgcataggca ctggcagggg gatttatgtg gaagacggct gcgggcacag catcgtgctt
     7381 ggtggctatt ccaggtcgga agtgcccagc gacattgtga tttccggcgg ccacgcgacg
     7441 gccacccggt gtggttcgtc agtccgggcg tcaccagcgg ggagatgatg ctggccagac
     7501 aagccctttt agatttccac aacttcctct ttcgtgatgg tattgtaggc cagtgttatc
     7561 cctccgaagc cggaaccggg ggccggagga ttgcccgggt caatgtcctt tgccagcaaa
     7621 tccagcgggt ataattccag tttggtgccg ggggtgcaga aaaataccac gtcgggattg
     7681 tccccggtct cgtccgtttt aaaaccaagc ttgtcccggt aaaattgcag ggactttttc
     7741 atgtctatga ctccaaggca gatgctgggt gctctggtca tgttcttctt catggaattg
     7801 gcgaatagcn nnnnnnnnna atagcctgtt tttctgttta atgttttgcg cagcctccgc
     7861 caggaaaatg tgtatgtccc ggttttgcta acgacgggtt ttatgccgaa agcaacggag
     7921 ccgtccgcag gtagttaagg aaccttttca tgaatcttcc ttcctcccgc attccctggc
     7981 tggacaacat ccgtgttttt gcctgtattc tcgttattgc cagccacatc gtggggcagt
     8041 tttttgtgtc gcctcctttc actccggaca atgaaacctt tgcctggtcc tccttttata
     8101 cggtgctggt gcgggtctcc atccccctgt ttttcatgat ttccggcgcc ctcctgctgc
     8161 ccgttcggga tacgacgggg caattcctga aaaaacgctt cacccgcgtc ctgttcccct
     8221 tcctgctgtg gtctgcgttt tacactgttt ttccgtggag ctatgaaacg ctcacggggg
     8281 acagctttca ctccgtcttc cccctctcac gggtcacgcc ggacctggaa accatgggaa
     8341 cgaacctgct gctgattcca ctgaaattca gcgtgggcat tcatctctgg tacctctacg
     8401 tgctgatggg cctttacctc ttcctgccag tcatatcccc ctgggtggag aaagcgggaa
     8461 aggcggcctt tgcctacttc ctgctgctgt gggcgctcac cctcttgttc ccttatctcc
     8521 agaccatttt ccccagctat ctgggaaaat gcccctggaa tgaatacggg gccctgcatt
     8581 ccttctccgg gtacctgggc tacatggtgc tcggctgctt tctgaggaaa taccctggga
     8641 atgcctcctt cgcccgcacg gcctgctggg ccgttccgtg tctggccgcg gccttttggt
     8701 ttaccctcca tttctaccgg acgtccacgg ctcaggcctg gtcctcaagc ggcgactaca
     8761 tcggcttcgc cagcattaat gtggccctca tgtccgtggc cttattcgtt ctcttccagt
     8821 cacttcccgc gttcggagcc gggtccgcgc cgcggcggtt cctggctgat ttctccagca
     8881 tgagtttcgg catctacctg gtccactttg tgctggtggg agccgtttac cgcctctttg
     8941 gcatgctcca tctgttgttc cttccggctt ctctttccat tccgctgctg actgcggcga
     9001 cgtgcctggc ag
//
LOCUS       DAWEMX010000108         8822 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3298_length_8822_cov_12.452492, whole genome shotgun sequence.
ACCESSION   DAWEMX010000108 DAWEMX010000000
VERSION     DAWEMX010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3298_length_8822_cov_12.452492"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1930 a         2839 c         2292 g         1761 t
ORIGIN      
        1 tcaagcccca tcatgacccg gttggactcc cctccctcca tgttcccagc atgaagttcc
       61 ccagaaccac cccgcttttc ttccggaaga agaacggggg atgacgcggc gttccttcat
      121 gaaatggatg ggtgcagggg cagccctggc agggataggg ctgccggcct gccgccgtgt
      181 ggaaaaatac ctggttccct ataacgaagg gcctgaatgg tctgttcccg gcgtggaaac
      241 ggcttacgct acctgcctga ccatgggagg aaacgcgcaa cctgtactgg ccgcctgtta
      301 tgaaggacgc cccgtcaaat tgattccctc cctgcaatac ccggaaggtc cggggttgcc
      361 ggctgcggct caggcctcca ttctggacct ttacgacccc ggccgcagca agcatatcct
      421 gttcaacggc aatccggcct ccgaagacga attccgggga gccttttctt cctggtcccg
      481 caatcttcgg gatggtggcg gcatcggcct tctccttccc ccttcggctt cccctctgct
      541 ccattccatg ctggaggaaa tcacccggaa aaattcactc gtgcgcatct accggtattc
      601 ccccgtgcct gaaccgggtt ccggcatgca ggcggggctg ccggaagaag tacggttccg
      661 cgtgcgtttt gcgcgcgcca ggcgcatcct ctccctggat tgcgattttc ttcatcaaaa
      721 cccctatgga aacacgcgcg acttcattgc gtcccgctcc ccggaagggc tttattacaa
      781 agaagaaaac aggaaccgaa cacgccttta cgccgtggag ggacgcgttt ccctgaccgg
      841 ggcccatgcc gaccacaggc tacccgttcc tcccgcacgc ctggcgtttt tcctggagga
      901 attgttccgc tatctttcca gcaaaaaaaa ctccggacaa acgcttcccc ctccccggcg
      961 aacggaccat cccctgacgg aaagggaact caactggctt cgccactgtg cggatgactt
     1021 attcagccac tccggagaaa gcgtgatcct cctgggggac aatcatccgg aattatccgg
     1081 catcgtctgg aagctcaact gtcttctcgg ctccatgggg acatgcatcc agttgctaaa
     1141 ggcaccgcgc ccaactccct atggcacgct ggacgatctt gttcgggaca tccgggggaa
     1201 aaaggtggaa atcgtttttt tactggatgc gggaaatccc gtgctggatt ccgggcacag
     1261 ttccggactg tcggaagccc tgaacagggt ggaaagcatc caccttggaa tgtatgaaga
     1321 tgaaacctcc cgcgtctgcc gctggcacct gccggcagcc cactttctgg aatcatgggg
     1381 ggtggagcgg gattaccggg gcagattttg ctatcgccag cccgtcattc ttcccctgta
     1441 cgggggcatt tctccggaag aagtgctttc cggcctgctt tcctccaaag gccatctctc
     1501 cacggcggac aattccccca cccacctctc tcccgtttac cacagagcgc gcaaatgctt
     1561 tgaacgcgcc gtgaatccgg aaaataaaac tgcggcctgg gctcaggcgc ttcagcgcgg
     1621 ctattcggaa gaaacggcct atgctcccct gtccccgcag gaagaaacgg ctcttggaac
     1681 agccatgatg caaacgcccg cagcgccttt tggcggccac gggaaaaaat tgggaaccgg
     1741 gatgctggaa ttgcaattcc gcgcggacta ttccattggg gacggacgct ggaaacgcaa
     1801 tgcatggatg caggaatgcc cggacccaat aacaggcgtc agctgggcgg cgtccgcaca
     1861 ggtttccccc gccacctttc tgcggctggg aggttcggat tccgggccca tgcactgcac
     1921 gcttaccgcc cccgccacgc aaatggaagt catcctgtgc cccattccgg gagtggcgga
     1981 taatctcatt attcttcctc tgggttatgg cgggatcaac cacgtagcgg aaaggcagga
     2041 aaattccggc ggatatgaac tccgcagaca ggtggaaaaa acggaagcat acggcattgc
     2101 cccggaacag attgccttgg ctgccctccg ggagcggacg gaagccattc aaagccccgt
     2161 tgtgcagcct gccccgttct ccctgcatcc cggtccgtcc cgcgtcccgc ctccccctcc
     2221 cggcgcggat gccgtttacc agtggaaaat ggccattgac acgtcccgtt gcatcggctg
     2281 caacgcctgc atgattgctt gccgcgcgga aaataacatt cccgtagtgg ggcgtgacca
     2341 gatggccagg ggccgcgccc tggactggat acgcatcgac aggtacttca cggaggaagg
     2401 gacgctcacc tccatccccg tggcctgcca gcagtgcggc aaggccccat gcgaatccgt
     2461 atgccccgtc aatgccaccg tgcatactgc ggaaggcctg aacgccatgg tgtacgcccg
     2521 gtgctggggt acccggtact gcgccaccaa ctgcccgtac aaggctcggc gcttcaattt
     2581 ttttgattac gccaaagcat cggaacaggc cacgcgcctt cagcgcaatc ccaatgtaac
     2641 cgttcgctcc cgcggcgtca tggaaaaatg tacctactgc gtccaaatgg tggaacgtgc
     2701 caaaatccgg cacaaatccc gcttgatgaa ggagcatccg ggccagccat ccacctccat
     2761 ccatgtgacg gcccaggaca tgctgcttcc ggacggggcg gctcaaacgg cctgccagct
     2821 cgcatgcccg atgggagcca tcaccttcgg caatgtgctg gaccccgccg cagccgtttt
     2881 ccgcgccaag tccctgccgc gccaccagga tctcctctcc tgcctgggca cgtctcccgg
     2941 cacgggctac ctggttccgg caagaaaccc caatccggcc atggagaaat aaaatgccct
     3001 gcccggaaat tgataaaaaa cggcccttcc ttaaaaaata aggccgcctt cttcaggagg
     3061 cggccttacc agtcaaaggt cttttgataa gctggtcact caatcttcca caatttgctc
     3121 cacggtcgta atcgtttcca ccacgtcgat gttggcggcg tcggcaaaat cattcaccgc
     3181 cttgagcaga gccggagtca tcagcttgtc cttttgaatg cgctcgcaat tttccagcat
     3241 atccacgatg gtgtcctggg cgtctccgtt caatttggtg atgcggatag cctgctcgga
     3301 agtaagcctc atcttttcca tggacttgtc cagctgcttg gcctgctggg tcaacgcatt
     3361 gagctgctta atggcgattt caaccgcagc gttgtccttc acgtcgtcca aaaccttgtt
     3421 ggcggaagaa atcaggtcga tattctgctt gaccatctgc acgggatcgg cagcgttctg
     3481 ggcaaaggcg gaagcggagc atacgatggc caccacaggg agtacttgat acgtttgaat
     3541 cattgtttta tcggtgttcg ggttgaagaa gaaacatctt ctttgcctct ttagattcat
     3601 tccggcggat aaatcgttgc cctcttatct gtcatgcgcc tcttacaagc tgaccgtcag
     3661 caaacggtag aaaaacaatc ttacccggcc acacgcctcc ccgccccgtc ttccagcaaa
     3721 aaacggagag ggcggtattt ggcggattcc agattgggat cttccgccag aatagcctcc
     3781 gccagctgcc gcccgcggtg aatcaggcgc gcatccagca gccattcctc aaaacgtacg
     3841 cccttcaggc cgctctggga cgtacccagc acgtctccag ggccgcgtaa cctcaaatcc
     3901 tgttccgcca ggtcaaatcc attcgccgta gtttccacga tgcgcaactt ctcccattgc
     3961 tcatcctccg gacgggcgtc cgtcatcaaa atgcagtagg acttgtgcga cccgcggccg
     4021 atgcgccccc ggagctgatg aagctgggaa agcccgaaac tttccgcatt attgatgatc
     4081 atcaccgtgg cattcggaac atccaccccc acctccacca ccgtggtgga caccagcacg
     4141 ctgatgcggt tggagcggaa atccttcatc accgcctctt tttcctctga agacatcctg
     4201 ccgtgcagca gccccacatc cacatgaggc agcagagcct tccactcgtc ccattccttc
     4261 gtcacggctt tccccttccg ggaatcctct ccgtcaatca ggggggaaac cacgtaaatc
     4321 tgcctccctt cctccagctg gttccgaacg aaagccagca cttttccctt atccttctcc
     4381 gtccggatgg ctgttacgac cgccccgcgc cccccgggca cgccgtccag gatggaaacg
     4441 tcaagctccc cgtaaaaagt cagcgtcagc gtgcggggga taggggtagc ggtcatcacc
     4501 agcacgtcgg ggcgctcttc ccggtcaatc agcttctccc gctgattcac gccgaatttg
     4561 tgctgttcgt caatcacgac cagccccaca cgctccggca catttttccc gtacagcagg
     4621 gcatgcgtgc caaccacaat cccgccctgc ttcccgaacg acacatggga ttcttccttc
     4681 ctgtccgccg tcaccaggga aacaggcaca tccagcttgt ccagcatctg ccggaacttc
     4741 aaataatgct gttccgccag aatctgcgtg ggcgccatca tcacggcgga ataaccgtgt
     4801 tccacagcca gcagcatggc gcacagagcc accagcgtct ttccggaccc gacatccccc
     4861 tgaagcaggc ggttcatgga acggggggct ttcatatccc ggtaaatctc cctcacgcag
     4921 cgcttctgag cctccgtcag ctcaaacggc agggaatccg ccagatcctt caccaaatgg
     4981 ctggaacctg cggtcctcat gccgggcact tcatccgccc gccttcttct gtaggccacg
     5041 ttcaactgct gagcaaggca ttcctccaac gcaaaacggc gccgcgcccg gtcccgggcc
     5101 tctgcggtct caggaaaatg aagatccctc aatgccgttt tgcacggagt gtccggcaca
     5161 aactcataca cctccggttc cggagccggg gaaagcctgg acagcgtctc ccatacgatt
     5221 tctcgcagcc tgcggaccgc aatccccatg cggccgccat acacgggaac aatgcggttc
     5281 aaatgaatgg actgctcatc tccttcccgg ataatttcaa aatccggatg caccatgctg
     5341 agctttttcc catacggttt catgcgcccg tatacaatca tctcctgccc ggcgcacaac
     5401 atcctggcta cgcccggcat gctgaaccac aggcaggaaa aacgggtctg ccccaaagac
     5461 tgctcatccg ccagcacggc ttccacatac cggccgcggc ccttgccgcc ccgtccgccc
     5521 cagcctttcc agcccacatc caccaccctt cctctcaggc agacgggcac accggaagac
     5581 agggaatcat agcggtcaaa catgcggcgg tcttcatacc tgcgcggaag catgaacaac
     5641 aaatcccgta cggaggccat tcccgccgcc tgcatggccg ccaactctct ggggaggata
     5701 aaaacgcatt cctccagaga agacgttaaa agcaattctc cagccatgaa cccaagaacg
     5761 tatcccttat catgcaccat gtgcgcgtac ccgtcagaac gctgcggcgg gccgtgtctc
     5821 cagacagcgc ctgtaataat ccacgcggga aatgaaccag ctccatttat gtcccggacg
     5881 gccgttggtc agcagcgggg ccgggcaatt cttccccgtc caataataat ggggtaccac
     5941 attacggagc ggaatatggt actgcttcat cagcactgcc gtcagcttgg cggagcggtt
     6001 ccaggtaaca atggggttat ggctccttac ctcccccatc tcaatgccga tggagtacat
     6061 gtctccgggg ccgccccggt ctgcgtggcg gcccgtctca ttcagcggaa tatgctggac
     6121 ggcacggtac tggtccaccg taaaatgcca gttcagggac cccatggccc cccttttcaa
     6181 tgcccgggca tgcgccgcgg cgtcaccctt gggattggct gtgctgtgaa tcgtgataaa
     6241 ccgcggatgc atggaatggg cggcccggcg agcacgggag cgcggggaca tgaaatcacg
     6301 gttaatgtgc acctctctgg acatctgggc catcgtgcgc ggcacgggag gcacattctg
     6361 aatcctgaag ctgacggcgg gcgggcgcgg ggcgtccgaa cacgccgcca gcaaagccgg
     6421 cagcatcaga aacaccgcat ttgccgacaa cattctgaca ttcatgattc ttccctagac
     6481 tatcgcagac gagctggcaa gcagatttcc agtcaaaaca gtctttttca ccctttccga
     6541 aacacggcag caggaaaaag ggatatcctg aagaaagccc ccgcgcacag ccatccggca
     6601 atcagggcaa cgcctttccc cgcatgcggt tttcatgccc ggagaaacaa aaccgcccca
     6661 taaaaagccg cacgggaggc ttgccaacgg ctatttccgt gcatacacta tcttcaccat
     6721 gagtaccact cacgaagcag ctaagaagtg ggtcgaagaa atcgcccagc tttgccaacc
     6781 cgactccatc tactggtgca acggctctca ggaagaaaat gacgagcttt gcaacatgat
     6841 ggtagaaaag ggcaccttta tcaaactgaa ccccgagaag cgccccgggt gcttcctcgc
     6901 ccgttccacc ccctccgatg tagcccgtgt ggaagaccgc acgttcatct gcagcgagaa
     6961 ggaagaagac gccggcccga ccaaccactg ggctgatccc gttgaaatga aggccaaact
     7021 gaagggcctc ttcaatggct gcatgaaggg ccgtaccatg tacgtgattc ccttctgcat
     7081 gggccccctg gactcccccc tggccaaaat cggcattgaa atcacggact ctccctatgt
     7141 cgtgaccaac atgcgcatca tgaccaagat gggtgaacag gttctcaagc gcctggaaga
     7201 cgaagtcaag ggcggcggca acccgaagcg tgacggttac gggacattcg ttccctgcct
     7261 ccacaccgtg ggcgctccgc ttgcggacgg ccaggttgac gtgccgtggc cctgcaacga
     7321 agaaaagtac atctgccact tcccggaaac ggaagaaatc tggtccttcg gttccggtta
     7381 cggcggcaac gccctgctcg gcaaaaagtg cctggccctg cgcatcgcct ccgccatcgc
     7441 ccgcaagaac aagtggatgg ctgaacacat gctccttctg ggcattgaat ccccggacgg
     7501 caccaaggcc tacgtcggcg gcgcattccc gtccgcctgc ggcaagacca acctggccat
     7561 gctcgttccc cccgcctcct accgggaagc cggctggaaa acctccatca tcggtgacga
     7621 catcgcctgg atctggcccc atgaagacgg caccttccat gccatcaacc cggaaaacgg
     7681 ctacttcggc gtggctcccg gcacgtccta caagacgaac ccgatcgcca tggacaccat
     7741 caaggaaaac gtcatcttca ccaacgtcgg tctgaccgac gacggcgacg tctggtggga
     7801 aggcatggac ggccccgctc ccgcccacgc catcgactgg cagggcaatg actggacccc
     7861 ggattgcgga cgcaagtgcg ctcatccgaa cgcccgtttc accgcccccg cctcccagtg
     7921 cccgacgatt gatcccgaat ggcagaaccc ggaaggcgtt tccctttccg cgatcctgtt
     7981 cggcggccgc cgcgccacca ccatgcccct cgtcttccag tcccgcgact ggacgcacgg
     8041 cgtttacgtc ggcgcgacga tgggttcgga aatgaccgcc gccgccttcg gcaacatcgg
     8101 tcaggtccgc cgcgacccga tggccatgct tcccttcatc ggctaccatg tgggcgacta
     8161 ctggcagcac tggctggaca tgggcaaaac cgttgccaat cctccgctga tcttcaacgt
     8221 gaactggttc cgcaaggaca aggacggcaa cttcatctgg ccgggctacg gcgacaacat
     8281 gcgcgttctc gactggattc tgcgccgcgc caagggcaag gctgaaggcc agaagaccgt
     8341 gctgggcgtc tccccgacct atgccgaact ggataagacg ggcctcgact acagcgaaga
     8401 acagttcaac gccaacatgg ctctcaaccc gagcgaatgg caggctgaac tggaaagcca
     8461 gaccgagctg ttcgacaagg tgggcaagaa gctcccggcc gaactggaag aacagcgcca
     8521 gctcctcatc aagagctttt cttaaaagtc cacctgactt aagaaaaaac tcttaaggca
     8581 gaggccgccc tttaacaggg cggcctcttt tattccgtag caacgaattt caaatccgtt
     8641 ttgcaagaaa aaaatctata attttctcat tttctgtatg gaacagtgaa aataaacaaa
     8701 cggaaaaaga aaccaatctt gtaataggaa tatttttaaa aatctattta ttaatgggtt
     8761 aagatagaaa aacattcaac gaatttgaaa atcgttgaat gtttttgcaa ttacgtgcta
     8821 tt
//
LOCUS       DAWEMX010000109         8629 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3386_length_8629_cov_11.242944, whole genome shotgun sequence.
ACCESSION   DAWEMX010000109 DAWEMX010000000
VERSION     DAWEMX010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_8629_cov_11.242944"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    875..884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2052 a         1797 c         2445 g         2325 t
ORIGIN      
        1 ataaaaccag ccgtattttt tctttcaccg gaagattttt cgtccggaaa atggaggaaa
       61 ggctgcatga aatggtccgg aaatggttga gagaatcttc ataacgggag gagatttttt
      121 ttggggttct gttaatcccc accatcataa gaaagcattg atggtaataa ttgacaaaca
      181 ggggggaatt gtgaagatca ggatagccag aggcagcgtg gacgatccgt tcataagcct
      241 ggaaacgctc ctgcattttt tgtgggttag ctccggatgc cattattgag ccgggacgaa
      301 ggttgtatcc atagcctcct aagccggatg aatataaaca accgattctt tttgccaggt
      361 cagggataat ataagtatct tcaaaaacgg tgccttctgg aaagcggagt tgggcaaaca
      421 cggcccggcg gaatatttta ttccatacgg cgtgattgac ttctggagac atgcaggctt
      481 ccaggatgtc acgttcacat tcccacattt gtgcagaatc tgcccctttg cggggattgg
      541 gttgattgta cggataaata tatgggaatt gaacgactgt gatatgttcg ttttgttcta
      601 gaatttcaag attgggcttg taagtatctt ccagaagttc atcgtcactg tccacgaacg
      661 ttatatattt gcctttgcaa atatccaggg ctctgtttct tgcggaagat atcccggaat
      721 tggcctgatg aatgaccttg atccttgcat cgcaaagagc cagacggtcg caaagggccg
      781 ggctgtcatc cgtagatccg tcgtcaatca aaataatttc catttccacg ttttgcttca
      841 atacggattc tacgcatttc tctaaaaatg ccgannnnnn nnnntatggg ccatatggga
      901 tgattttatt tattaagaac catgtttttt aaatcatata tttcctgaaa tctgaagaag
      961 agcgataata gaataaaaat ggctatgtaa atacaggaag acattgccag gatgaaaaag
     1021 ggattttccg ttatgtaaaa ggcagggagc gcacagaaga aggaggcaat gctgaggccg
     1081 atgatgggaa gaatgtcttt aatttgcttt ttgatcccat attgaatcag tctcttggta
     1141 tagtaggaat taattcccag ttccagaatg gatagaagaa ggaggccgca gcacatgaaa
     1201 aaaatcccga aaggtatggt ggcgcacagg atcagtacgc ccaggatttt tttccatact
     1261 tcaagacgca ggaaaagatc gcttcttcct ttggcttgaa gcagattgag gttgagggcg
     1321 ttcattggag tgagcgcgag agagagggat aaaatttgaa gaaaaggcac acaaggaagc
     1381 catttttctg ttaagagcgc agaaacgaga ggggatgctg taaggacgag caaaagcatg
     1441 caggggagaa tgatatatgc catcatgcga aggattctcc ggaatacatg ttggagctgt
     1501 tcatcattat cctgtatttg actcatcaaa gggtaagtaa ctttggagat aatggaatac
     1561 agggaagaca tggggaggcg ggccatggtg ttggcacgtg tataatagcc tagatccgct
     1621 gcagagaatc ttttggcaat gacgaattga taggcattcc ggtaacagga atcaaggagt
     1681 gacgaaatca tcagcttaca tccgaatcca aatgtcgaat gaaaggattt ccatgaaaac
     1741 tccaaactcg gtttccaagg tacaaaatac caaagtgcaa cagttgttaa tacggaagat
     1801 aaaaaaccct gtagcacgag agcccatact ccgtaaccca tatacgctag aaaaatgccg
     1861 ataatcccgg agataataaa gatgggaatg gagaaaatag tttgtttttt gaattccatg
     1921 ttggcgtgga gcaatgtgcc gtgtacagct cccaaggctc ctataatcag ggtaagtccg
     1981 gaaatccggg taatttctgt taaaagaggt tcattaaagt aggatgctat gaatggggcg
     2041 gaagtgtata gaactaggta aattaaaata gagacagcag tattgtaata aaaaacggtg
     2101 gcaagatctg tttgagtccg gtctattttt ctgataagtg ccatgctgag tcctccggtt
     2161 acaaaaatgc ccgaaatttc tataaaaata cttatagttc ctactaggcc atagtctgac
     2221 ggagtcagca aacgtgccag aataacacta atgagaaaac ttacgcattg gttgccaaat
     2281 gtgagaatag cactccattt gactgcggtt tgagcattct gtttcaggtt attcataggc
     2341 cgttttttat gattggagaa gaagtttggt tgatttccag ttatttcttt tccataaagt
     2401 ataggaacgg gctctttaag gacaggattt tatggaatat gcgggatatg tcataatgcc
     2461 acgggtaagg gaggttgttt tttctgaatg cctgtttgtt ctggcgcgta ttccgcaaga
     2521 tggcgcgcag acccgatgtg ctggagccgc cagtgcgcat gaataccatg tcgcggtcga
     2581 tataagtggt ggagatccgg tttttttcta taaaacgtag catcagctca aaatccgccg
     2641 cactgccgat agaggggtca aagccgccgt aacggataaa gcagcccttt ttacaataga
     2701 atgtggggtg tgccgggctc cagccgcgct ggaaggatcc aggactgtat tccgttccgc
     2761 gccatgtgcg tacggtgtcc cttactccgt tgatgtagtg cagattaccg tgtacggcat
     2821 ctgttccatg ccaggcaaat gccctggcga tggctttcag tacattttcg tcctggtaat
     2881 agtcatccgc attcagaaaa ccgatgacat ctcctgtggc catgcctatc cccttgttca
     2941 tggcatcata aattcccttg tccggctcgg atagccattt catgcggccg gaaaattgat
     3001 gactgtattt ttcaatcaat tccacggttc cgtccttgct ggccccgtcc accacaatgc
     3061 tttccacgtc cgggtaggtt tgccgcagaa tgctttccag cgtgtcttgc agcgttgcaa
     3121 tgctgttata acaaaccgta atgactgaaa ttttcatttt tggcgggggg agtggaggaa
     3181 atttattgga cgcttttctg acggctcctc atgataagct gggtatttgc ccttagacct
     3241 ttggtcggca agtctttggg ggtcaaggga tgggcgggta cgctcaagga ggggacaggt
     3301 tcttttcgga tacttcttaa aatgagttgc gtattttctt tcaatccgta ggtcatcaaa
     3361 tctctgggct tcagtacacg ggtcggtgta ctcagggcat ccggtcgctg ttgaatgcct
     3421 tctctctcga caaccttgag gaaggtgtaa aaaaggatat tcaggtcgta ccggaaggtc
     3481 aggtggcgtt ggtagctcag gtcataacat acccgctgac ggaaggtgag gttgttgcgc
     3541 ccgcgaatct gggccagccc cgtgatgccc gggcgcaggg acattctttt tttccgggtg
     3601 gaaggggtaa aaatggccat ttgatcggga atccaggggc gtgggccgat aaagctcatg
     3661 tcccccaaca gaacgttgat gagttcaggc agttcgtcca gggaggtgct gcgcagcagg
     3721 gagcccaaga ctgttgtgcg ttcttcatcc gggagcaggt tcccttcctt gtccctggtg
     3781 tctttcatgg ttttgaattt gatgatatga aagggtttga ggccgtgccc gatgcgcttt
     3841 tgcaggaaga agggagatgt cctctggacg atggccagca gaatagccag aatggctaat
     3901 accggaaaca ggcatataag gccgaagagt gctcccgtga aatccagggc gcgttttccg
     3961 tagcgtctgt aaaaattatg attacgatac agcgggttgc tgaaggggat attagttgta
     4021 ggggctgaca tgggtgtagt acgagaggtt gttgataggg taggcggagt cagtttttca
     4081 gaggcatgcg tttggccatg gcgatgttgt ggctgtctgt cccccaccat tcgtaaagtt
     4141 gacgggtgcg gaacgtttgt gccagggctg cggcggattt gccgtaaaat cctttcaggg
     4201 agccgacatt gccctgatat ttgacgcctt cctgccgggc cagttggagg aaatcttcct
     4261 gttgcaggag gcggccatag cgttcagggt gcgccaggac agggacgtat cccatgtccc
     4321 gaacggagct gatcatgtcc ttccaggcgc caggaaggta gtactggggc agttccacca
     4381 gcaggtggat tccgtcgtag gtcaggggag tgtatcggcg cagctgctca tggaactggt
     4441 tgtccagcat gtattccgcc gcgagccgga gctggaattg ttcttctctg gtgtcggcaa
     4501 gcaactggtc aaaacggcgg cgcaggaaat cgggcgtatt gtccgggtag cgcgccatga
     4561 tatggggagt gcagtaagct cctttgagtc ccatggcctt gagccggcga atgattccca
     4621 ggctttcttc aaggcaggcg gctccgtcgt ccactcccca tagaatatgg gtgtgcatgt
     4681 ccatgccgtc cggaaagagg ctggatgtcg cataccgggg agtgaacagg ttgccgaaca
     4741 tgattgctgc cgaaaagagt aaagagcagg gaagaaagcc ttgggcttat gcattgccta
     4801 tgtcttatta atcttgtttc ctggtggttt gccgtggttc tccggagccg tatccgtatt
     4861 ggtaaccgta tccgtagcca tagtagctgt aactggagga cttgaagttg acgccgttga
     4921 tgatgtacgc catattggga agttttccct gatcggccag ttgttggatc tgggttaggt
     4981 atcgtttgtc gattttcccg ctgcgcacgg tgtacagggt gatatcgcta tgggatgcca
     5041 gaatggcggt gtctgccaga atgccgtagg gcggagcgtc gatgatgacg gcatcatact
     5101 gttttttcag gatgcccagg atttgaccca gcagaggatg actgagcagg gtaaccggat
     5161 tgggcaccat ggggccggtg taaaggatat ccatgccgcg ggagttttcc cctagggggc
     5221 gaatggtgcc ggtggtgtcg gagctctgtt ggagcagaat ggaagtgagt cctttgcgtc
     5281 cggaaccgcc gagacgccgg gtcagggagg atttgcggaa atctccgtca ataagcagta
     5341 cttttttgcc ggcataggcg aaagcggcgg ccagattggc ggaggtgaag gttttccctt
     5401 ctccggggag ggtagaggta accagaatga cgtgtcctcc ctgttccggt ctggggagca
     5461 tggaatctac gttattgcgc aggatgtgga agcattccgc gatgacggaa tgttcgtcct
     5521 gaatgaaaag accggtattt ctactttctt ttttgttcat ctggggcagt tcggcaatga
     5581 caggctgcct gtttgtggac gggagatcgt gtttattgtt aaccttgctg ttcagcatgg
     5641 ccgctcccat gaaggcaaat atgctgaaca gggcacctcc cgccgttcct cccgcgatgt
     5701 attgaatggt ttttggggaa atgggaaagt tggacccatg ggcggtttcc agaacccggg
     5761 cgctggattc cgttaccgcc atagccaggg cgttttcctg ttccttgctt aagagcatca
     5821 ggtagagttc ctccttaacc ttgtgttcgc ggaccagggg aatgatttcc tgctccctgc
     5881 ttgctgtctc cgtgagtcgt tcagtcaggg aattccgttt gctgatgagc tggttgagct
     5941 tgagattcaa attgctgcgg tagttggaaa gagctttgtt ggcggccgcc ctggtggaat
     6001 tcattctgtc gcgcaagctc accgtaattg ggttctggga accggcgctt cctgcaattt
     6061 tctgatattc cagataggct tcgttatagt gttctatctg ccgggcaata ccggaatcgg
     6121 gaagtccggt ttccacggaa atcaggccct gtttgcgttc ggtttcctta agattgtcag
     6181 ccaggatggc tgccagtttg atttgtgttt cgagatcgaa aatttctttt tccagcgcct
     6241 gggacgtgct gaagtccgcg ctcatggtgg tatccgtatc cttgacaatg tcgcttttcg
     6301 tcttgaattc ggtgatcttt ttgtccacgt cactcagggc ggctccgagt tctttcaggc
     6361 ggctctggat gaagttttcc gttttaaggg ccgccttgtt tcgttcatcc ttggaaattt
     6421 ggttgtaaac ctggatgagg tggttaagga cgtcttccgc tttctgggga ttactggccg
     6481 tcagggacat ttccagaagg ctggattctt tggcgtccgg acgggtgact gtcagtccgt
     6541 ggagcaggtt gttagcagtt tcaagcacgg gcttgtgccg gacaatgaca gttttttcat
     6601 tccacgcatc ggtcatcagg gatgttggat ggatggaaat gacggcaaag ggaagtgtaa
     6661 gaggttttcc gtaaaaccct tccagctgga ctgattctcc ctggttattg gaatggctaa
     6721 gcatgaacct cttttcatct agaggcgtga tctgcagggt acaggctcgt tgcttatcga
     6781 tccgttcaaa atggaccagc agggggctgg ttttgtagag gtcgatttcc ctgaaatctt
     6841 ttttttgcca ataggaagtg ttgaggttga ggtcttccac cacttttttc attaatgcgg
     6901 tggatttgag gatgaagctt tcgttggcca ggtttgctgc gccggaatcg atgcccagct
     6961 ctgccataat gcgttccgaa gatgcgtcac tgctcgtttt ggcatcgcgc atcaggacgc
     7021 tggccgtctt ctggtaaatg taattctgtc ggccggctag gtagtaagcc gccgtacctc
     7081 cggcgagggc tgccagaatg atgataaacc agtagcggcg caggacgagc agcaccgtgt
     7141 ccagggaagt tgtttcttct gtgttttccg taacaggcgg agtgggagcg gtattatttg
     7201 ccatggagga aagagggtgg aatgttgtgt ggaaatactg aacccctggg gaatcggtcc
     7261 gatacaatcg gaccgtccct atatttccgt tcacaggtat gattaagccg gacgaaaaag
     7321 cggcggaatg tccgttacag gcacagggcg accacggcca gagtaatgcc aagcccggag
     7381 agaccccagc cgtaccattg agcggcatcg ctgcgtgtgt tgaccttgcg gtctgagggg
     7441 gtcacgtaaa tgatgtcgtt ttgctggatg taatagtaag gagaagtgaa caaatccttg
     7501 ctgcgcaggt cgatactggc gatttgacgg gttccgttgg tttccctgat tagcgtgacg
     7561 tcccggttgc cgtcaatatt caggtctccg gcccggctga tggcctcaag aatggttacg
     7621 cgctgtcctt caatagtgta ggttccggga gcattgactt cgcccagaac ggagaatttg
     7681 aagttcatga tctgaacatt gacggaggca tcccggatgt agtcattatt cttgagagta
     7741 gcggcaatgt cggaggccag ctgggagcag gttttgcccg cagccttcat tttgccgatg
     7801 acgggcagga cgatgcagcc ctgggcatct accaaatatc ctttgggttt gttactggag
     7861 gtgccgctgg aggaaccgat ttcagacatg gcaaaggggg cggtgagctc cggctgcttg
     7921 ctgctgactg cgatatagag ctggtcatcc ttttggatgg ttgtctggta gtttgccttg
     7981 atggtttccc tggaatttcc ggaaacatcc tggatataga ggacttcctt ggggctgacg
     8041 caggaaggca ggagagcggc tccggccatg gccagggagc acagaatggc tttattgatg
     8101 aggttgtttg tattcatttg gtgagggagg aggaaacgtt gcttttggga ggaaagatgg
     8161 tgataaaaac aggggaagag gccattccgg ctcaaaggat tttcttcctt gccccggaag
     8221 caccacggga atggcgtgat gccctccatg ctttcaatct tccagaagtt tgaaagaaaa
     8281 aggccgctat atgttcagtc attattttaa atttgatgat tttgattttt tttgtttgaa
     8341 acaagcaagg gtttgaaaaa gggggatata tttcccctca catggaaaaa gcggggaaat
     8401 aatcatcaat aatgaaaaga tactggcaga agcagtaccg ccgaacaaaa taccggggag
     8461 tggccgctga cttgcctgca catgaaagtc gtgcctggtt aacggattgt aatttcaatg
     8521 tcatgaggaa gagcattctc aattagcgga tttcaaatcc tattaaaaaa ataggaatat
     8581 tatgatttag tataactaga taatgattag cgtttaaaaa atttttaac
//
LOCUS       DAWEMX010000110         8569 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3420_length_8569_cov_13.085389, whole genome shotgun sequence.
ACCESSION   DAWEMX010000110 DAWEMX010000000
VERSION     DAWEMX010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3420_length_8569_cov_13.085389"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    567..576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5638..5647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1486 a         2107 c         2700 g         2256 t
ORIGIN      
        1 gcgtcccgga tgacggattt ggcgttcgcc aaaatggcca gacgggagag gatatcctgg
       61 ggtacgtcgt ggggagtttt caggatgaag ctgacgtttt tattgttgga aaggtcgtat
      121 tccgctttca ggttgcgggc cttgttggcc gtttcataaa gggctgcggc ctgggtattc
      181 gccagggtga tgcggttttc atcaagtccc gccaggaggt tttcggcaga ggggagagga
      241 gtgcgcatga gcggcaggcc gccgttgctt tccgcaaagc ccagggaggc ccagagttct
      301 tccgtgatat gaggcatgac cggatgcaga agggccaggt agtgcctgag caccgtgtcc
      361 atcgtggtga gcgtggctgc ctgtgccgct gggtccgccc cgtccttcag gtcacccttg
      421 acggcttcca ggaagcggtc gcagtattcg ttccagaaga attggtacag ggtttggatg
      481 agggcgttga attcgtagtc cgccagcgct ttttctacat ctgcatgcag ttgcttgagc
      541 ttggagataa tggagatatg tacggannnn nnnnnngtga ggggccgttt ctcccctggc
      601 atctccctgc atcagccgga agcgcgtggc attgtacagc ttgttggcaa agttgcggcc
      661 ttcgctgatc tggttttcat caaagcgcac gtccgttcct gtgggggcga tgcgcatgag
      721 tccgaagcgg aggccgtccg ctccgtagtt ggcgatcagg tccagaggat ccggggagtt
      781 gcccagggat ttgctcattt tacgcccgat tttgtcacgg atgatagacg tgaaaaagac
      841 gttgctgaac ggtttgtcat gctggaaacg gtatccggcc ataatcatgc gcgccaccca
      901 gaagaagatg atgtccggtc ccgtgacaag atccgtagtg ggatagaatt tggcgcgggt
      961 ttcctcatcc atggtggaga aaggccacag ccaggaggag aaccatgtgt ccattacgtc
     1021 gttgtcctga atccagttgt ccgggtcctg gggcggttcg gttcccacgt agaggaagcc
     1081 ctgttccagt gcggaggcgt ccagcgccgg ggcgttcctg agttcttccg ccttgtcttt
     1141 gcggtaccag accgggatgc gatggcccca ccagagctgt cgggagatgc accagtcctg
     1201 aatgttttcc agccagtggg cgtaggtttt cgcccagcgc gcggggcgaa aagtgatatc
     1261 ccctcccgct acggcgtccg cggcttcttt cacgcagggg tatttgagga accactgcat
     1321 ggagaggcgc ggttcaatcg ggacattggc gcgttcggag aacccgactt tgttatcata
     1381 gtcttcaacg ttgaccatga ggccggaggc ttccagcatt tccacggatt tgcggcgtgc
     1441 gtcaaagcgg tccatgccgt ggagttcggg cacttccggg cagttgatat gaccgtcggg
     1501 ggtaaggatg tcgataattt ccaggttgaa tttccggcct atttcaaagt cggccttatc
     1561 atgggccgga gtgattttca gagcgcccgt gccgaattct atttctacat attcatctcc
     1621 gatgatagga atgggggtgg gattgagcgg gcgcatgacg tttttcccga tgaggtgggc
     1681 gtaccgcgga tccttcgggt tgacggccac agccacgtcc gccatgatgg tttccgggcg
     1741 ggtggtggct acatggaggg ctccggaccc gtcttccagt ttgtaaagaa cggtgtagag
     1801 cttgcttttc tgctcagtca tgatgacttc ttcatcagag agggccgtga gggatacagg
     1861 gcaccagttg accatgcggc ggccccggta gatgagtcct tccttaaaga gttcaatgaa
     1921 gacctgtcca acggctttgg tgtattcctc atccatcgtg aagcgttcgc ggtcccagtc
     1981 gcaggaacaa cccagcttat ggagctggtt gatgatgatg cctccgtgtt tttctttcca
     2041 ttcccagact ttttccagga aggcttcacg gcccagatcg tggcggctcc ggcccgttgt
     2101 ctgtttgagg tctttttcca cccttacctg cgtggcgatt ccggcatggt ctgttccggg
     2161 cagccagagg gcttcgtatc ctttttggcg cgcgcggcgc accaggatgt cctgaatggt
     2221 gttgttcagc acatggccca gatgcagcac tcccgtgacg ttggggggag ggatgacgac
     2281 agagtaggca ggcttttcag aagccgggtc agccttgaaa caaccttctt caatccagcg
     2341 ggattgccat ttttcttcca ccagggaggg atcgtatgct ttgggcagat cagacatggg
     2401 caaaagggta gcataaagtt tttttctttc aatccctacc tgtccgggag agggtggaaa
     2461 aatggctctt tttccccgga ataatgtgat gaaaaagccg ccgggtttcc tgatggagcc
     2521 cggcggctgg aacggagttt ttcctgtttt tttatttgac cgtcacagtt gtctgagccg
     2581 gcttgagtcc gttggagatg acttggagcg tcacttttcc ggattctccc ttgcgggagc
     2641 gcagaatgac ctgggcaaga ccattgaagg ccttgatttt catgattttc ttgttttccg
     2701 gattcgccat ggtggtgaag tccgtgggat caccattgca gatgccggcg atgtccgcag
     2761 ggccgcttac cctgaaggtg agctggtttt tgcttcgggg caccatacgg ccctgggcgt
     2821 cgcggacggc tacggtgaca taagacaggt catagccgtc tcccttgatt tcattgcggt
     2881 ccggcttgag ggtgagtgcc gcaggttttc cggtagtggt taccgtgtcc gtagcccaga
     2941 ttttaccgtc ctttttggcg actactttga gggtgccggg cgtgtatttg acgtcttccc
     3001 acacgaggcg gtagcggtcc ttttccccgg tgcccttttt gcggacgccc tgggatttcc
     3061 cattcaggaa gagttccgct tcatccccgc tggtgtagac atgcacgggt gttacctgcc
     3121 ccttgcgttc cggccagttc cagtgcggca ggatgtgcgc catcttgaga tccggcctcc
     3181 agtgggcttg gtagatgtag aagcggtcct ttttgaagcc gcacaggtcc acgatgccga
     3241 agtaggagct gcgggagggg gcgcggtctc ccagtttttt gagttccgcc atcagctttt
     3301 cgcgttccgg cccttccggc acgttcaggg cgttggtggc gtccagattg tacggggtgg
     3361 gttccccaat gtagtcaaag cccgtccata catattctcc cgcgctgttg ggattgtctt
     3421 cctgagcggc gaattccaca tccggacggt tggcccagcc gggggcgtac aggtcatagg
     3481 aactgacctg gtagaggtag aatcccttgc ccttgttcca gtccacgggg aagaagtatt
     3541 ctccgcggga gctgacgcag gaggcggttt ccgcaccata gaacggctgg tgggggcggt
     3601 ccttggcgaa gtccttatag gcccagggct tgtagttgta gccgtaaaca tccagggtgt
     3661 ccccaaagcc gttgcgtgcg gcattggcgt tgttgcagcc ggaagtcact ttgcgcgtgg
     3721 ggtcttcccg gtggaaaaga tccgtcaggg tttgggagat atgatgcaga tttttcattc
     3781 cctgttccgg aacttcattt cccgaactcc atgcaatgac acaggggtgg ttgcggtccc
     3841 ggtggcagag gttaaccacg tcccgttcat gccattcgtt aaaggattcg ttgttgacgc
     3901 cttctttgga gcattgccat acgtcgaaaa gttcgtcaat gactaggatg cccattttat
     3961 cgcacaggtc cagcacttcc ggcgcaggcg ggttgtggga cgtgcggatg gagttgacgc
     4021 cgaattcctt caggatttca atctggcgct catagcctcg cgtgtgggcg gcggagccga
     4081 gcggtcctag gtcatggtgc tggcaaacgc ccttgagctg cacgcgctcc ccgttaaggt
     4141 agaatcccgt gggtttccat tcaacggttc ttacgccgaa gttggtgaat ttttggtcga
     4201 tgactttgtc tcccatcctg accgtggttt tcatcttata cagatgggga gcgtttaggg
     4261 tccacagagt ggggtttttg agcgtcagcg tgctggtgat tttgcccttt tccccggcgg
     4321 gaatttcttc ccctttggtg gttgtggagg ctacgatttt acctccgtcc aggatttctt
     4381 cagtaacagt ggggatgacg gctttgtccg tggtgttttt taccgtggtg tccacttcca
     4441 ccttggcggc ggatttggtg atttccggag ttttgacgaa aacgccccag tgttcgatgt
     4501 gcacagggtt ggattccgtg agccacacat ggcggtagat gccgccgccc ggataccagc
     4561 gggaggtgct ggggggattg tccactctga cggcaatggt attttgttgc ccgaatttca
     4621 ggaagggcgt gatgtccacg cggaaggagc tgtaaccgta tttccattcg ccggccaggt
     4681 gtccgttcac ataaattttg gggcgggaca taacgccgtc aaagtccaga tagaattggt
     4741 tgcccttggc gtccgccggg atttccagcg ttttgcggta ccagccaatg ccgacccagg
     4801 ggagttttcc ggttctgttt tcaatttcca tccggaaggg gccttccacg ccccagtcgt
     4861 gcggcaggtt caaggaacgc cagtttttgt cgttgaaaga ggggctggag ggattgtccg
     4921 acttactggt tccggcggcg gcctgagggc ggataatttc ccccttgtcg tttttaaagg
     4981 tgatttcacg gatgctggcc cagttgctct ggttgttgcc cgtgacggtg atgcggaagt
     5041 atcgcggttt gccggttttg ttttctaccg tgtcttcctt ggagtcgttt tgcccggaag
     5101 ttttgtcttc aatagttgcc cagcgttttc cgtcaccgga gccttccagc ttgaaaagat
     5161 ggttgctttg tttttcccac aggatgttaa cggtttttac atctacgggg cgtcccatgt
     5221 ccacggtgat tttttctccg cttttgccac tggcggcaca ccagcgggtg gacttgtccc
     5281 cgtccacggc attgtccgcc ggattgccgg attcttcact ggtggcagtg gcgaatccca
     5341 tggcttttcc cggttccggt tgcgtactgc catccggcat tttcccgaaa cgggcgaatt
     5401 tccagttcca atccaggctc tccgttttgg ttgcattcag aggcgccgcc atgaggctgg
     5461 cgatgctgaa cgcagtaatg cagagggtcg tgcgtgtaat attggtgaac atatctgtga
     5521 tattacgact ggaaatctgc tatcttgcaa gatttttatt cagcaggata cggtttctgc
     5581 ccggattacg tcagcagggt tccacgcgtc aggcgggatg acttgcggcc gccgtccnnn
     5641 nnnnnnngta tgaaggcgtc ttccagagat atgggcgaga ggaagatgcc cccggcagga
     5701 gaaggagagc ggacggaacg gttggcggaa agaaaaacag ggggaatgaa agagagaacg
     5761 ccggaccggc cttgttgaat gggccggtcc ggcaatgggg aaaggagaaa aatcagatgt
     5821 atattttgac gatggctttt ttccggaggc gttccaccca ttttttataa cgttcattgc
     5881 tgcgcttgtt gcgcgccatg atgtcaatct gctccttgat ggcggagagg gggggcggcg
     5941 gcgccagttc ctttttctcc accaatacga tggtgaagcc ggtggagtcc accaacggtc
     6001 cgatgatttc gccgggctgt gcgccaaaga tgatggcggc ggattccggg gagagcgtgg
     6061 agcgttccgt aacgggccag tcaccgcctt tttccgcata tagatccttg gagtagcgtt
     6121 tggccatttc ctcaaaggtg gcgtttttgg atttgatttc cttggcgatg agttccgcca
     6181 ggttcagctg tacttccggc gtggaggcgg agtcatcccc cagcatggga atgaaaattt
     6241 tcttgaattt gattttgtct ttggtcaggt cccggtattg ttctttggta gattcgtatt
     6301 cctgctgtat ttcctccgga gtaggcggta tttcctggtc gtatttggaa gcccgcatga
     6361 tttgaacctg gagctgtttt ttcgtcattt cccggaaagc gcggatggtc atgccggaga
     6421 ggctcaggtt tttcaggaag gcggaacgat ctccgttaaa ggtggtcagg atggtgcggt
     6481 tgacttcctg gtcaatgaga gagtccctaa tgacgccgcc catgccttca aattcattgc
     6541 ggagcagttc tcgctccacc aggtcttcaa tgatgttttt tttcgccagg gccagttgct
     6601 tgtagaattc gggcccctgt ttgggatact gggccgcgag ctgtgctccg atgggcatga
     6661 gacgcacgct gacctcattg gccgtgatgg gacggccgtt gactgtggcg gcaatccggt
     6721 tgacgatccg ctggggctga acacgggatg ccggcgcttc gttcggagag atgcgcttgg
     6781 attcgtctgg ccgcaccgtt tccctgcctt catccccatg aagctggacg gctcgtggaa
     6841 gggaggcccg tgaatccacg atgctttgcc cctgtgcgga caggggcatg gtacagatga
     6901 gaagcgcctg gatgtgggtc ctgatattca tggggggaga gaagagtatt tttaatgcct
     6961 ccggcatgat aggggacagt cctgcaaaga caatctattt ctgggtatgt atggatgctg
     7021 cccggcatgg gcgggatgcc ggttcctgca cttttgctga ctgtggatgt gcttggtcat
     7081 cgccgttttt ccctgtacag tatgcggcga tgcatgattg ccgtttattg agaggagtgc
     7141 tccgttcctc ccgtccggcc aatattccca cgctgtttac caacgcggcg gcggcgtggg
     7201 cttccgtccg tggggcggag ctgccgcctc tcgccctgta tgggggcgta gtgctgatgg
     7261 ggctgtgctt ttatttgtat ggcatgtggg agaatgaccg tgtggacgcc aggtgggatg
     7321 ccgcccgtta tccggaccgc cccgtgccgt gcggcgcggt gagcgtttcc gtactgaggc
     7381 tgttgtcttt ggcggccggg ttgtccggcc tggtgctgaa catggcgctg ggcggggaat
     7441 ttgccgtggg ggctttgctg ctgattgtga tttccctgta taatgtgttc cataaactgt
     7501 ggagcggctc cattgttttg atggggttgt gccgcggggt gtgggtgctg gccgccggcc
     7561 tggcatttgc ccgttcgggt ggagagtccg ttcctcctcc ggccttgctg tggtatgctt
     7621 tcggcctgtt tttgtttacc tgcgtgattt ccgccgtagc acggcgtgag gcgggcaggc
     7681 cccgggttca aagggctgtg acggtgctgc tttccggcat gtgcctgttt gatgcggtat
     7741 ggctcctgag tttcggttcc ctgctgtggc ttggttccgt cttattatgg gcgggaacgc
     7801 ggctgttgca gaagctcggc tgccgggcca cataggtttg agttgaattt acatgggaag
     7861 tgtgcatgat ctaacgcgag aaagagggtt atgaatgaat tgatcatcaa gataggtccg
     7921 ttgttctggg ttttgagcgt tctggccgtt tatgcgctgg cggttgtcgc ggagcggatt
     7981 ttgtattttc acaggattca aattaatact ggagattttt taagaggtat ttccaagctg
     8041 gtaaatgccg gaaagacgga tgaagcccgg catgaggctt ccattttacc gggacctgcg
     8101 gcccgcgtgg tttcttccgt gctggcgcat gccggattga ggcgtgaaga actacggact
     8161 gtggcagagg attccgttca gatggaagtg tttcagattg aaaagaatat ccgcggattg
     8221 ctggtagtgg ctacggtgag cccgctagtc ggcgtgctgg ggacgatcca ggggctggtg
     8281 ggattttatt cccagccggg ggttctggaa gggaaggctc cctctcttgc catgtcggat
     8341 gccgtttacc aggccctgct tagttccgcc ctggggctga gcattgctat tcccgcttat
     8401 ttgttttatt cctacctggc ctcccgctcc cgccagattg tacattccct ggaacgggcc
     8461 ggcacggagg ccgtttgcct ggtgtgtgat gcccggcaga ggcgcgaggc cggagagagc
     8521 atgaacggcc ggggcgtgtg agtgcaacag ggaaggagga aaggcatgc
//
LOCUS       DAWEMX010000111         8557 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3430_length_8557_cov_11.070807, whole genome shotgun sequence.
ACCESSION   DAWEMX010000111 DAWEMX010000000
VERSION     DAWEMX010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_8557_cov_11.070807"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3808..3817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1970 a         2519 c         2282 g         1776 t
ORIGIN      
        1 tgcccgtatc cagccacgta taaccgcgcg ccagtttttc cacgcgcaac aggccctgct
       61 tcaggtattc ctgattcaca gaggtgattt ccaactcgcc gcgggaagac ttgggaatcc
      121 gtttggctat ctccaccacc cggttgtcgt aaaaatagag gcccgtcacc gcaatatccg
      181 atttgggcct gagcggtttt tcctcaatgg aaaccgcctt cccctcctca ttgaattcca
      241 ctaccccgaa acgctgcggg tcatgcacct ggtaaccgaa aatggtcgct ccgctcgtca
      301 gttcggcggc cctcctcagc ttctccgtca ggccgcgccc gtaaaaaata ttgtccccca
      361 gcaccagggc aacggaatcc tttcctatga actcctctcc aataataaag gcctgagcca
      421 gcccctccgg cctgggttgt tccctgtatt caaaggaaac gccgaacctg ctgccgtccc
      481 ccagcaaatc ccggtacagg ggcagatccc ggggcgtgga aataacaagg atttcccgta
      541 tcccggccag catcagcacg gagatgggat aataaaccat cggcttatca taaacgggca
      601 gcaattgctt gctgacggaa agcgtaacgg gatgcagacg cgtgccgctt cccccggcca
      661 gaacaatacc cttcatatca aattcctagc cattggttcc aagacgttcc aggcggtatt
      721 ccccggacag gatgccccgc caccattcct catggtccag ataccactga atggtctttc
      781 tgaatccgga agaatggtcc tggcgcggag tccaccccaa ttccctccgt attttggaag
      841 cgtcaatcgc atagcgcagg tcatggcccg gcctgtcctg cacaaaggag atttgctccc
      901 ggtagctccg gccgtccggc cggggccgca gttcatccag atgggaacac agcagctcca
      961 ccagctccag attggttttc tcgttattgc cgccgatgtt gtacgtttcc cccggttttc
     1021 cgcaggaaac gaccgtcaac agagcctcgc agtgatcccc cacatacagc cagtcccgca
     1081 cgttttcccc tttgccgtat acaggaatgg gttccccgcc cagcgctttc aggataacga
     1141 cgggtatcag cttttccggg aactggtagg ggccgtaatt gttggaacag ttggtgatca
     1201 gcacgggcag accgtacgta tcgtgccagg cgcgcaccag gtggtcgctg gaggccttgc
     1261 tggcggaata cggggaatgg gggcgatacg gcgtggactc cgtgaacagg ccttcctttc
     1321 ccagggagcc gtacacttca tccgtggaaa tgtgatggaa gcggaaacgc tcctttttct
     1381 ctccctccag ggaacggtaa tattccaagg cggcctgaag catggaaaaa gtgcccatga
     1441 cgttggtggt cataaaattt cccggatcgt caatggaacg gtccacatgg gattccgccg
     1501 ccaggtgcat cacccagtcc ggagcgtact cccgcaaagt gcgccgcatc agggaagcgt
     1561 cccggacgtc ggcgtgcaga aagcggtatg ccggagaatc cgccgagccg tccagtgaat
     1621 ggatgttgcc ggcataggtc agggcgtcaa tattcaggac ttcatggccg cgctccagca
     1681 gcagcctgac cagattggag ccgatgaatc cggcgccgcc cgtaacgata actttcatag
     1741 cactgttttc agggaaagat gcggcgccat catacagcag cggcgttttt tttcaaaaca
     1801 aatccgccca tgccgcggga acgtggcggc atgggcggga aaaagcgggt tccgtaaccg
     1861 ctgagacggc tagcagctca gctcccggca aaggttgatg atatcctggt tcacggagta
     1921 ctgctgcccg gctacggaat ccagggaggc atagccgtac tggccgtcat gatacaccat
     1981 gatctggttg atgtccccgt tgttcagagc ttccacggcg cgggtggcaa aacgggtgcc
     2041 catcaggcgg tcacgggcgg tgggggaacc gccgcgctgc acatggccca gcacggtcag
     2101 gcgggtatcc atgccggcgc gttccgtcag ccagcgggtc aggtactgcg ccacgcgggt
     2161 gccttccgcc accacggcaa tgatgcagct tccttcctca ttgatgacag gctggaggcg
     2221 cttgtaaatg gattccaggt catacggaat ttcagggaca atgcagatat ccgccgcgct
     2281 gcaaagagcg gtggtcatcg ccagatagcc gcagtcccgg cccatcgttt caatcacgaa
     2341 agcgcggtga tgggaacggg ccgtatcacg aacggaatca atgcaatcca gaatcacgtt
     2401 ctgggccgta tccacgccca ggcagtagtc cgttccggca atgtcgttgt caatcgtagc
     2461 cggaataccg gcaaaaggca cttcaaaatc attgaaaaag tcgttcaggg cgcggaagga
     2521 accgtccccg cccatgatga tgagcttgtc gatgttgcgt tccttcagat gttcgtaggc
     2581 ctgcttgcgg aattcgtagt cgaaaaagcg cttggatcgt gaggaacgga ggagagttcc
     2641 gcccctgtag atcatatcgt tggtcagttc ccgggtggct tccttgattt ttccatcgat
     2701 cagcccttcc aagccgtcat aaacaacgta cgggttcatc ccaagctggc gggcatgatc
     2761 cactgccgcc ttgatcgccg ggttcattcc ggcggcatca ccgccagatg tcataatggc
     2821 tattccactc ataagtaata aggttaactt gtccgggggg aaatgttacc gggcggaaca
     2881 aataatgcaa gcgcggagtt tttactggcg caataaacgg caacacttca tgatgcggag
     2941 gcatgaacga cactgcagca gccattctga aagccacggc cgccctccgg gacggcacgg
     3001 agaagctccg gttcgaatcc ccggtccatg ttacctacaa ccctctcacc tatgcatggg
     3061 ggccgcatga acagtatgtg cgcacctacg gaaacgggga aaagggccat ctgttcctgg
     3121 gcatgaatcc cggccccttc ggcatggccc agacaggcgt ccctttcggg gaagtggacg
     3181 ccgtcgtcaa ctggctgcac atccgcggag aagtcgggcg gccggaacat acccacccca
     3241 aacgtcccgt ggagggtttc ggctgcccca ggtcggaagt cagcggccgc cgcctctggg
     3301 gacttttcgc ggaagctttc ggcactgcgg aaaacttctt ccgtcacaat tacgtggcca
     3361 attactgtcc gctgatctgg atgggagcca ccggagccaa cattacgccg gacaaactgc
     3421 cggcggaaca gcgcgccgcc gtggacgcgg tatgcatgga acacctgctt tccctcatca
     3481 ccatcctgaa tccccacacg ctggtggggg tgggagccta cgccacgcag aagctgcggg
     3541 acgccgcctc ccggctcccc ggcaaaagct tcaccatcgg cacgttgctc cacccaagcc
     3601 ccgccagccc catcgccaac aagctctggc cggaacgccc catccagcag cttaaggaac
     3661 tgggcatttt ataaaaagag cggaggaggt agagtggaaa gctccgtgac atggaaggaa
     3721 tcaccagagc gacaggccgg aaatcggcag ttcatcttgt tctcctccaa atctacatcg
     3781 ttttgaagga atatccggcc ccctttannn nnnnnnnttt actggcttga tttgcccagg
     3841 gacacggcct tcctgacgga agaaaagcac gccggcatct accgggacgg agaagaactt
     3901 aaacacctcc ccacttccac cacaaaaatc agcctccctc caaatcaatc atcattctta
     3961 gactgaaaac cctccgctct tccccgcagg cctttttact ggacaggggg aaaagggttc
     4021 cgtacagttt ccgcgatctt caagcgttcc gaatttatgg caatttcaga ttacatcgtg
     4081 accattggcc tggaagtcca ctgccagatc aagacaaaga gcaaaatgtt ctgctcctgc
     4141 gagaccggct ttggctttga acccaatacg cgcgtgtgcc ccacctgcct ggggctgccg
     4201 ggcgccctgc ccgtcctgaa tgagttcgcc attgaacgca cgctgctgac cggcctgatg
     4261 ctgggatgtt ccagcccgga gatttcccaa tgggaccgca agaattattt ctatccggac
     4321 atggctaaaa attaccagac ctcccagctg gatcttccgc tgtgcctggg aggggaagtg
     4381 cccctgtacc cctggtccta tccgaatgac gtgatcaaag ccggcgtccc ccctttccgc
     4441 accgtgaagc tgacccgcat ccatctggaa gaagacgcgg cgaagattac ccatcatgcc
     4501 aattattccc tgatcgacta caaccgcgcg gggtccgccc tgatggaaat cgtttccgag
     4561 ccggacatcg acaccccgga agaagcctac gcctacctca agtccctcca gcagattctg
     4621 aattacgggg atatctccga cgcggacatg gaaaagggcc agatgcggtg cgatgtgaat
     4681 atttccctgc gtccccacgg gcagaaggaa ttgggcgcca aggtggaaat gaagaacatc
     4741 aactccatgt ccgccgtccg cagagccctt cattatgaaa tccagcgcca ggcggaagaa
     4801 ctggacgtgg gcattcctca aatccagtcc acccgccgct gggacgacga acgcggagaa
     4861 agcgtcgtca tgcgaaccaa ggaagatgcg cacgattacc gttatttccc ctgtccggat
     4921 ctggttcccg tacgcacggc cccgctggtg gagaaagtgc gcgggcagat tcccgaactg
     4981 cctcaggaac ggcagaaacg cttcatggaa gaatacggcc tgagcgagta cgacgccaac
     5041 gtgctggtgg gagacctgga actggcacgc ttttttgaag aggcggcgcg gggaaccgcc
     5101 agcggcaaaa aaatcgccaa ctgggtgatt aacaacattt ccgccgtgct gaatgaaaaa
     5161 ggcatgcgcc cgtctgaatg cccggtgaaa cccggcgcca tccgggagct gatcgccatt
     5221 attgacgacg gcaccgtttc caacaaccag gccaaggacg tattcgccaa aatgtgggat
     5281 gagccggccc tcgccgcagc gcaggccgcc aagctgctgg gctttgaaaa ggcggattcc
     5341 tcttttctgg acggcatcgt gcgggaagtc atttccgcca atccggacaa ggtggcggaa
     5401 atacaggggg gcaatgaaaa gctcctgaac tggcttaccg gccaggtcat gaaggccgcc
     5461 aaaggcaaag ccaacccgaa aatcgtgacg gaagcgctga aaaaagccct ttccatggag
     5521 tgaccataaa caaagagccg cttcccttgc cggtgccggc tccttgcccg cagggcatca
     5581 cggtttgaac agcttcctgt ttccggccag ggtgttccat acctgcttca cttccccggc
     5641 cagcgtctgc ttcatcatgg caagggaaaa gtccgccgcc tggcccatac tgatgtaggg
     5701 aggcagggca agggcgtccc tgtccacatg cgcgtccagc agagcggcgc ccggataatt
     5761 caggaacatg tccaccatct ggtccacctg gctggatttg tccaataaaa accccttgat
     5821 gccgacggca tccgccacgg cggcaaaaga ggggttgttc aggttgatct gccacggaat
     5881 cagcccggcc gcctgcattt ccaattggat gaaatccaga cagtcattgt tatacaccac
     5941 gattttcacc ggcagcctgt actggacaat cgtgatgaga tcccccagca gcatggacag
     6001 gccgccatct ccgcacaggg caattacctg ccgttcgggg tattccatct gcgccccaat
     6061 agccattggc atggcattgg ccatggagcc atgattgaag gaagccagcg tcctcctgcc
     6121 cggcgtggcg cacaggaacc tggcgctcca gatacagggg gtgccggtat ctaccgtaaa
     6181 tacggcgtcg gatgccgcat gcctgttcag ggcggtcgtg agctgttccg gccggatggg
     6241 agactccttg tcatttcctt ccatataggc gttcatctcc ttcacgtccc tggcgtggcg
     6301 gttcagggcg gcgtccaaat gcccggaatc cgtcttttcc tgcaccatgt tcagcagagc
     6361 ctccgccgtg acggaaacat ccccgcaaat acccaggtcc aggcggcagc ggcggcccag
     6421 cacttcaccg cgcctgtcaa tctgcacgat ttcaggtttg gtgggaatga aattgaaata
     6481 ggggaaatcc gtcccccaca acacgagcat gtcgctttca tgcatggcct tgtaggcatc
     6541 tccccatccc agcagccccg tcatgccgat gcccagggga ttgtcatgtt caaaataatc
     6601 ctttcccctc catgtgtagg caatcggagc tttcaggcgg tgcgcgagcc ggacgacctt
     6661 gtcttccgct cccgcgcagc cggcgccgca gaagaaagta atgcggcggt ggtcgtcaag
     6721 catccgtgcc agaagggata cggattcatg ctcaggaaca acattctccc gcgtgtagga
     6781 aggaggatgc accagattct tcatctccgc aggggcgtcc gcaatgtctc cgggcaagac
     6841 gacgacgcct acgtcgcgcc tggcccacgc gtgctgaatg gcggacatca aaatggacgg
     6901 ggcctgggaa gcttccgaaa ccagttccgc atatgcactg cattccttga aaagctgttc
     6961 cggatgcgtg gcctggaaat attccatgcc tacctggctc tgcgggatgt gggaagcaat
     7021 ggccagcacc ggggcatggt tccggtgggc gtcaaacagg ccgttgatca aatggaggtt
     7081 cccggggccg ctgctgccgc agcatacagc cagattcccg ctcagctggg cttccgcgct
     7141 cgcggcaaaa gccgccactt cctcatgccg tacatgaatc cattcaatgg tcttcctcct
     7201 cctcaaagcg tccgtaacgg ggttcaggct atcccccaca atgccgtaaa tccgcttaac
     7261 tcccgccttc tccaaaattt caacaatctg atcggctacc ctgtgcatgc agattagaca
     7321 ccagccaacc tccaacggat tcattcttat gtgtcatccc ctccatcttt tccgcaccgg
     7381 aaaaaatgcc tgaggatttc ccctgatatc ataaggccgc cgccttttca ggcagcggcc
     7441 tatgaattac cagttctctg gtggaaaacc gtcaggaacg gcggcggcgc atcatcagaa
     7501 cggccaggcc caggattccc agagaagccg aggccggttc cggaacctga aggccggcaa
     7561 aaccggcaat ctgttccgcg gaaagttttg tatccgtcca ctgaatttca ccccatgtaa
     7621 gattgggatt aatccatcct tggatatcag tgcgatttcc gttcatgttg ccgttgtagg
     7681 acaacgtgtc aagcaaggaa ccattcacat agacctccac agactggctt tggtttgcgc
     7741 cggtaacggc tagagtcacg ttaagaagac ctccctccaa aacaacggga gtttcagaaa
     7801 cgtctccacg gttttgggaa aaagagccgg tagaagtatc caaatttcca tctcccatgc
     7861 caagcttggc atctgtcgtc agatacaagg catttgtccc gtaactttgg cccgcattgg
     7921 tgccataata cccaaacagc gtggtctggg ttccttccgt taaggaagag gtatccagcc
     7981 agagggagat cgtgtaattg gaagcataaa aattaccgcc gtaaggaatg aaaccgccag
     8041 gaaggacact ctgtgagaga ctcccaaacg tcatgtcttt tcccaatgta ataccttcag
     8101 ttgtcgtcgc cgtaccggca acgtaagacg ttccggccga tggttctaaa gagccgggag
     8161 acgccaacgc cggaacggaa caacacgccg caatggccat taatgctaat gtttttttca
     8221 ttttctgtga atgatggact gagtagcaat cagagagata ttccccgccc tctgtatatc
     8281 ggattcccaa tccgctcttt ttctccccca caggaaagat gagaacaagc tttttcaatc
     8341 cgctaaaaaa tataatataa ttaccttcta tcacttatga atataaaaaa taaaacgcag
     8401 aatagatttt ttgtggaggt tctgaagttg ctgtatagga acagcaatcc cttttcgcaa
     8461 aatagaaaaa attgtaataa tagtttcata attgaaatgt tagattgacc tcggattggg
     8521 aatccggttg ttttccctca gccatcaaaa aaatcaa
//
LOCUS       DAWEMX010000112         8434 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3482_length_8434_cov_12.952620, whole genome shotgun sequence.
ACCESSION   DAWEMX010000112 DAWEMX010000000
VERSION     DAWEMX010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3482_length_8434_cov_12.952620"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1932 a         2520 c         2220 g         1762 t
ORIGIN      
        1 ttgccggaac gcagaccgcc caccactcct gcgggagcga cggccccggc cacattggcg
       61 gcgctgtcca gcacggaatt gttatagtca tttttatcgg ttcccgcgcc gcccatgaac
      121 cacttggtag ggtccaggtt cagattgacg atccagttaa agcccaattc ctccaagtcc
      181 gtctgattga cttccagaaa ggtcgcgctg accaccacct ggaggggctg ttccgcagtc
      241 ctggccgcta ccagctcctc cagcatgctc aaatctctgg gcgtgccatg gaaaagaaga
      301 gtggagttgc ccgagttgta tttaatgaaa ctgccttccg ggaatttcac ccccatggaa
      361 gccagcgcct tttgcggatc cacccgtttc agggtcatgc cggaagaacc ggaatcaccg
      421 gaactgaagg gatctgaatt ttcggcgcca gagtcctccg acaatccgga aaagaagccc
      481 ggcggaaggg taatggactt ggtgaccatg taggaagttc cgtctgaact ggataccagt
      541 tccgcaccaa aggcatttgt ccgcagaata aggccggatg cgcgcgccac gtattccaag
      601 gcctcacgaa gaggtacatt ctgaagatta agcgtaattc tcttggcggc ggcgtccctg
      661 gcggaaacgc tgtctgcagg accgggatcc acggaaatat tcacgccgcg ttccgccgca
      721 gtcatggccg tggcatcatg cgtcctggcc tggctgcgca gatagtccac ggcttcctgc
      781 accgtcgctc catccagctg cacacggggc aggcggatca tgcccagttt ctggtccaca
      841 ttcaccaccg ggttatccag cggccgggaa aatgaagcgg gttcctccgg tgtttccgtc
      901 aaaggaacgg gcatctccca ctgtctggaa acatccgcca gggcactgct gcgggtttca
      961 tccctggcag cactaaaata ggcggttctg gcgcggttaa ccagttccat gccgcgccgc
     1021 gccgccgtat tgtaagggtc cgtcttaagg agggacgtaa attccttcag ggccgcatca
     1081 tactgaccca gttcataata gccgaaagcc aggtgaagca gacggttgac ttcctctaca
     1141 ttttttacgt gttccggaga caatgccgga ttcgtacgca ccgggtcgcg ggctatttcc
     1201 aacgtgcgcc tgaccagggc atggtccgga gtggatttca gggcatcttc cagcaacttg
     1261 acggcctcat cgtaacgccc cgccttgata tattcctgag ccacagccac actggcatcc
     1321 gcgatgctgg tttccaggaa acggcggcgc ttctccatgt tgggggattt gggcagcgca
     1381 gtcaggcttc tgcggtaatt gtccagagct tcctggtact tgccctcgga atattgctga
     1441 cgcgcctgcc ccaacagcag catggaagcc tgagcctgct cttccatccg ggctgctgcc
     1501 cggcgtgcgg cccctgcccc ctcctgggcc agcccctggc cggctccaat accggcaacg
     1561 gcaagcgcca tggcaatttg ctgaatggca gacatggagt gagggaaacg tctagtcata
     1621 ggaatttccg ggactatacc gaattaaaaa ccgcccgtca tcataaaaac gcccagcaga
     1681 gtaaaaaata tcggcaaaag ctgcagcttc cctccggagg gaacgtttct ctaaacggca
     1741 gacagttcca gcacgcccac atgtcccggt ttcaggggat gggacagacc gcagtccatc
     1801 aattcccgcc gctcaaaaat cctctcctca tcctcggcgt ccatcaggtt tctgacgcgt
     1861 acccgatccg tctggatgcc gcaccagccg aaagcgtgct ggggaatgga aatgcgcagg
     1921 ttgtagaaat tgatatttgg agaaaggttc cccaccacaa gcacggtggc cctggcgcgg
     1981 gcatcatgcc ggagcatggc atatacccag tggccggaag tatcttccgc cggttccctg
     2041 ccgaaagtgg tgtttttcat attagcccag ttcaggccgt aaaaatctcc ccggtccagc
     2101 gccggatgct gcatcagagg aagaagcttc cgatgaaaat ttctcagttc tgcggactct
     2161 tccggcagca gggaagagtc aaaccggcca tccgccaccc agggctggag ccgggggagg
     2221 caggtatagt caaaaatact ggtacgcccg tcatgtccgc cgaaaccgcc gggcccctcc
     2281 gcccgttccc ccacttcctg cccgttgtac accagcacgg gaccgctccc ggaggcatac
     2341 accagcgtca tgaccgccgg cagaacaacg cgccccacgc cgccccagga aagaggggag
     2401 cacacgcgcg tttcatcatg gttctccacg tagcggacgc cgtgggtcat gtatatggga
     2461 ttgcttcgga aaagccggtc gaagtcattg gcccagttat tttccgtgta catatggagc
     2521 gccagatgat agcagtcgga atcgtaaacg gcattaaatc cggcatccag aagggcggcg
     2581 cacggatcgc cgggtgtggt tttcatgtgg tcgttgtatg cctccgccat gaaaaacacg
     2641 tccggcagac gcacccggga acgggaaatg gcccatttcc agaaagccat ggggatcatt
     2701 tgggccatgt cgcacctgaa accgccgatg cccaggcttt gccagaagga aaggatatcg
     2761 tccatgatgc gccatgtctt gggcacatgc tgcttggggc tggtccaccc cggcaacagg
     2821 cggagggcag gaagaccggc cataaagtta tagccgtagt tgagcttgac cgtttcatac
     2881 cagtcgtact tgccgggatt ccatgtaacg gcattgttgc ccgtcacacg tccgaacgtc
     2941 atttcccctt caaacaggcc gtccggcaga cgcaggggtg gaccgtcccc agggctgttg
     3001 gaggtcaggt aaaagtaccc ctgctccgga gaaaaaaacg tatggggatc atctccttct
     3061 ccaaaatcgt catgcccgtc ccagtccgcc aggtaggctc gggaaacatg gttgggaata
     3121 aaatccatca tcggcagcag ccccaccgtc cggcaacgct ttaccagagc cttgaactca
     3181 tccattctct tctccgggga ggaggccagg tccggatcca cgtcaaaata atccaccacg
     3241 gcatagggac ttcctgccag gccttttacg atggattccg gctgggcggc cagcccggga
     3301 tgggccgtct gcgtagcgtg cctcaatacc ccggtcagcc agatgtgtgt gaaacccatc
     3361 cgtgctattt cacgtaaggc tttatccgtc actccattaa aagttccgca cccgttcgtt
     3421 tcccggctcc cccagtcaac tccatgggtc tccatattgg aaaaatgacg aacaaatagt
     3481 tgataaatga caggacgcat gagttgaaat aatcaaattt tgacgcttat aatgctaact
     3541 ttttcaggta ttctgcaagc gcattctgtt ccaccggggc aatttgtccc agctgcagca
     3601 gggcttcctc cctggtccgg agcgttccct ccaactgaag ttcctgcaga cgggacagcc
     3661 atttcccgaa atcaggcccc ggggacagcc ccagattcac cagatcacgc ccggtcacca
     3721 gaggaggagg aaccagaggg gcattccggt aggaatccat gaattccctg acaaattgcc
     3781 agttgtccat cagtccgttg gaggaaaggc aatccaggcg gtggagttcc agttcatccc
     3841 ggaaatgagg agcgttcatg aacattcgca gcgtagattt cctcatctgc ctcacgttga
     3901 tgaagcgcat atggcgctcc accatggaac acaccgcatc caccacggca ttggaatatt
     3961 tcagccttct taaaatgacc ctggccatgg aagaaccttc cttgtcatga ccggaaaaac
     4021 ggatgcgccc cgtttcatcc acctgacggc agggaggttt cccgatgtca tgcagcagaa
     4081 cccccagggc cagcttcaaa gacacggggg agccgtccct tcccagcgca tccagcatca
     4141 tcagcgtatg ggtgtatacg tctccttccg gatgccattg gggaggttgc gtacagccta
     4201 tcatatcgta tatttccgga atgacatgct tcatcaaccc cgtttccacc agcatttcca
     4261 caccccgttt ccttccggga gacaataaaa tcctgtccag ttcctcccgg atgcgttccg
     4321 gggaaatccg ggccagcagc ggagcgttcc tgaaaatgga ggagcaagtc tccttctcca
     4381 cctgaacttc cctggtaacg gcaaaacgta cggcgcgcat cagccgcagg gaatcttctt
     4441 caaaacgacg gtccggctcc ccgatgcacc tcaatacgcg ggcccggata tcctccactc
     4501 cgcctacata atccaccaca cggcccggag gcgaagaaaa cggatcttca aagaggccgt
     4561 tcatggtaaa atcacgccgc cgggcatctt cttccgcatc cgtgaaacag acgtcctccg
     4621 gcctccggcc atccctgtag cttccgtctc tgcggaaggt cgccacctca aaggaaaaac
     4681 cttcgcggcg taccagcacc acgccgaagg cggcgcccac ttcccaggca tccggaaaca
     4741 ggcgcaacac ttcatccggg cgggcggagg tggcaatatc gatatccttc acggaatatc
     4801 ccagcaacct gtcccgaaca cagccgccca caaaatatac ggtgtgtcct gctccggaaa
     4861 gaacccgggc cacgtcctcc gcggcctttc tcaacagaat cccctccatg aaacggatca
     4921 ttcaggcatg ggcctcaaag gaggagcgga aagagaatgg tcctccgcac cggagggggc
     4981 ggaagggaac gcgggccgcg gcctatcagg ccgggaaaca gagggaacgc gcgtatccgg
     5041 ggaattaatc ctggccggcg gagaaacagg cttggaggaa gcaggggagg agggagactt
     5101 tttcccatcc ggcttttctt tcttttttcc ctcaagtttg cccatgaccg cgtacaggtt
     5161 ggcgtcaaac agcatatccg gcgtgccgcg catcaggatt ttccccgcag ggtccagcag
     5221 atacacccgc ggggtgagcc tcacccggta atccttaaac gccgccgctt tttcatccac
     5281 cagggagggc atatccattt tcaagttcaa cagggccttt tctatgttct ctctctttcc
     5341 gggagccacc ggcatcaccg tcaaaccggg aaattctttc cggagagacg cggctttttg
     5401 caaaaactga acggaacgca tatcctgcgg atcccaaaaa accaccagca cgggcttatt
     5461 cccggagggg agcgtcactt ttccggccgc cgcggtagag ggaaggctga tttcaggccc
     5521 ggtctgcccc atattgagat tgcgtatttc atacagttcc tccttcacaa tattttgaac
     5581 cggaataggg ccgaaactgg aatcatagca tttgataatg gcgtctttca gcaattttcc
     5641 gcgggccgcc accagcctga cgtcatcctg aagcatccct gtcgtttcct tcatcaccat
     5701 ggccaggccc agggaagcca ggccctggtc ctcagggaac tggttgtaat ccttgatctg
     5761 ttccagaata acgcggcagc gcaggtcacg ggaattgctc agggcatacg ccgcctttcc
     5821 tgccccctgt tcccggaaca gcgtattttc cagggaatcc aggcagaaca cgacaatttt
     5881 cctggccatg tctccattgg ggaacgcctg cgctacagcc tggggatgat ccagaaacca
     5941 gacgacagcc ggaagaaggc aggatttttg agtctcgggc ttctgaaggt cgcgccctac
     6001 cagcctccat aattcccgag ccaccggaac ggcatccgga cggttcttca tcaattccgc
     6061 ccttttttcc ccgctttcag ccgttttcaa tgcggattcc cagtcggact gcctcatccg
     6121 ccaatcctcc agcacattgt tcatgcccgt ctcgtccgcc ggagcccatc caagcaggct
     6181 gcatgcaccg gctatcacac aaaacgctct cttcataccc tgccactatg ccctgacgcg
     6241 tcccacacgt caatcaaacc ccgtggcaaa acgcagccta ttttttcttt tcccctccct
     6301 tccagctgtt tacagcgcgg aatacatctt ccggaaatga acaaaccccg gcgaatccct
     6361 tcgccggggc tgctgttcaa ctaactacct atgaagacgg ttgagtcctt aagtggatga
     6421 agcgctctct ctttcactgc atccggatca taaccgttat gggtgagaca aacacacggc
     6481 aaatatgttc aaacattatt tcttttgtat gacacaaggc ccgggaagac aaaaaagcgg
     6541 ctacggaatt tctcccgtag ccgctccaac taactaccta tgaaatcagg gttattcctg
     6601 gtgacaaggg aagctctcgt tcctccctta tcgaacatcc ctgttcattc aggatacctt
     6661 cacgaattaa acgctcaaga atttttttcc ttacgtgaaa aaaacagaga accgccgctt
     6721 caggccgcta caaagtttcg ttcgtcattg gtcagggccc gcacctgcac ggccataaac
     6781 ctcatttcat taagcatgtt cagataaagc agggaggaac gggtacgcgt ctggttgcgc
     6841 tgggcccgct tgatctggcg cttggtggcc tttgccacca gttgaagaag ctgttcctgg
     6901 gccaccatcg tatagtcgaa ctgaatgtac gtccccgtct ggagcatttt gcaatattca
     6961 tccagcagct tgatgagggc ggtatgcacc tccttcaaat cgtccacctg gtcaatcgta
     7021 aacggcgtgt ggttattatc cacatacgtg aagatggact cggaaaactg cgacaggctc
     7081 tgcgtagctt cattcaaatg ctccagggcc tgcacaaagt ggtagcccat atccagggag
     7141 cgggactgca tcttgtacag aatagggaga atatcgtact tgtgatgttc gctgctggtc
     7201 atggccagcc gttctgcagc atccgccagt tctttcaggg ccttcctgtc cccctggaaa
     7261 aagttttcca cggtgtcatt gtaaatttcc cgcagcttga gcgtgctgtc cacaatctgg
     7321 tggctgcata catccaggat attttcttcc gtaatctggt caaaacgctc tacggaaagc
     7381 ttgctttccc gcttgcggtg catgatggtg gactttacca gaatgacaac caccagggcc
     7441 agcagaccga agatggcgta cacctgtccg taatgcagga tggtagccat gacggcggcc
     7501 agcgtaaagg cagccagccc ggtcatgaac cagccggaaa tcaccgtcag cacgccggtt
     7561 acacggtaaa ccgcgctctc ccttccccat gccttgtccg ccagagagga gcccatcgcc
     7621 accatgaacg tcacgtacgt ggtggaaaga gggagctgcc tgccagtagc ccatgcaatc
     7681 aacagggaag caaccgtcag gttcaccgtg gcgcgaatca ggtcaaatgc cacccggtcc
     7741 ttgggattca agggctgggg ggcgggatcc cactggtggt tcatgcgcac cagcatgcgg
     7801 gcgggaataa gccttttcac cccgcgtccc agggcgatgg cgctgcgaac gatggcgcgg
     7861 gcggggggga cggagccgaa acgctcaata ccgccttcac cctgcctgga aagttctacg
     7921 ccggtggcga tcaccgtttt tgcctttttg gagaaccaca aggcaaaaat catcatgcag
     7981 cccgcggcga acaaaatata atggtttacc ggcatgccct gccccggcat cagacactcc
     8041 atggaggtga tggatgaggg agagccaccg gccgccgcca cgctgctggc gtactggaag
     8101 gaatccagac cacccatgaa aacgccgatg aaattcacca ggtcgttgcc cgcaaaagcc
     8161 agggccaggg aaaaagtgcc cgccagaacg gtcaggcgca gaatattcgt catgaacaaa
     8221 tgctgaagca ggaacatgag cacggaaaaa ccgccgaagg ccgtgagcag ggccatgccc
     8281 agattctggt caatgtattg gatgaacgag ggctccatca gggtagactc cttcaagccc
     8341 ttgaaaacgg caaaataggc tatcgccgtc atggcaatgc cgcaccagaa acagccgaac
     8401 catttgaacc gggactggta tttaaacgtg aacg
//
LOCUS       DAWEMX010000113         8285 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3547_length_8285_cov_12.446416, whole genome shotgun sequence.
ACCESSION   DAWEMX010000113 DAWEMX010000000
VERSION     DAWEMX010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_8285_cov_12.446416"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1556 a         2172 c         2713 g         1844 t
ORIGIN      
        1 ggtgcctttt gtggccggcc agttcgccca gcgctggatt ggcaaatggg tagtcacgca
       61 ccggaacctc acttcctgga cagaccacag caccatctgg ctggttattt acacggcatt
      121 cagccatgcg atgattaacg gcgtatggaa aaatctgccc tggctttccc tggtggaggt
      181 gctggctttt tgcggccttc tgctggccgc ggccctgtgg ctgacggctt tcctttcacg
      241 caggctccgt ttttcccgcg aggaccgcat tgcgattatt ttctgcggct ccaaaaagag
      301 cctggctacc ggcattccga tgatgaacgt gatttttgcc ggagccccca tcgggctgct
      361 ggtgattccc atcatggtgt tccaccagct ccagttgatg gtttgttcct tcctggcccg
      421 gaagtgggcg aaggacgtgg gaaagagaga acaactttaa gatggcctct gtttgcctgc
      481 cctctcaatg tccggtcagg cgcaggactc cgtcgtattg catgtcccgg cccggaacaa
      541 tgatgtggct gccgcgggaa ggaacttttg cagtaacggg tttgttgcgg aactgggcca
      601 aaaaccgttg gaaggattcc atgtcttccg gaatgatttt tacttccgta taggcggggt
      661 tggaaggcgc attcgtgatc tccacccgga aggaacccgc gatattgccg tccggcgcat
      721 agaattccaa tgattcttcc ggaggggagg gatgcatgaa aaacaggaag aacatgatgc
      781 ccaaggccgc cagggtgacg acagtcagcg gaatgttttt tctgagggga ctgatatgca
      841 tgccttcttt tcctttcttc cataagatta tgggttttgc cggagtgtgt caacccctcc
      901 gggggacagg cgatgatgca ttccttcccc atattctttt caaggaacct gttccggaag
      961 aacgggagac ggaaaaaggg ttgcgtccgc gggacaatgt ggtagcttgt gcgggcaaag
     1021 ggaaccctat gaccttacct gtaaagattc tgtccgtatt ggcagggctg agcctggcgg
     1081 gaagcgcccg ggcgcgcatt tggacgaatg accggggcgc taccgtcgta gccgtgctgg
     1141 tggccgtccg tgatgcggag gtggatttga agcttcagga tggtcgcgtg gtggctgttc
     1201 ccaagaatat tttttccgga gccgaccagg aatatattct ggaatgggta aagtctggcg
     1261 ggacgctccc tgatacggat gtgggggctg agaatcctcc tgtgcagggg gattccggca
     1321 gacggtacgt tcccctggag ccgaattggg atgctccctg gccggagagg gtggttgttc
     1381 ccggtttcct gctggtgaaa accgtgcagg agacggagga gctgagtgtg tatgaaacgg
     1441 atcatttcat catggagtct cccggaaagc tgccggaagc ggagcgtatg atccttgccc
     1501 ggcgttttga aaccattctg tctgccctgg cagctgttcc gttgaatctg gcggtggccc
     1561 gccggccttc ccgcaagtat ctggtgaggg tatgtttcca ggaagaggat ttcaacaggg
     1621 ctccgggcct gcggaacggg catctcaagt tttcccctac cagtttcacg gctctgctgt
     1681 tgcgggataa gaagggaaag ctgctgaagc cgggactgga tccgcgcttt gccgtcacgc
     1741 actgggcggc gcagtccatg gattgggatc actggctggt ggacggcttt tccgcgtaca
     1801 tggcttttct tcccatggag aaggaagctc cggtattccg gaagattccg gaaaggttgg
     1861 ccgcgatggt tccgcgcgcc gtccgcacgg gcagagaagc tctgccggcc ctggcggaca
     1921 tgctgtccag ggattcagca cattccgccg cggagcatgg cgtttcctcc aggggaggaa
     1981 cgcttcagta ttgggccgat ttgttgtgga tggtttactg gagccacctg gaagggagcg
     2041 gaaaggcgga gcgcctcaga agctatttga gggttcgtga tgcggagggc ggcgggaagg
     2101 cgcgtgctgt tttgctggat gggaagactc cggaggatgt tcagggggaa atggccgcgg
     2161 cctggaaaaa aatgggattg aggctgagat tttctcagcc ggcttctgct gccgcggaca
     2221 gggagtctgc gggaaaataa cgggaaggga gggaagaaaa actcctgccg ggaaggtgtg
     2281 ggaaacctgc gtctcaaatg gtgccgttaa gttttctccc tatccagaac agggtaagca
     2341 gaaggatgag gatggagagc gtatacgggt tgcaccagag caggatgcgc gtttccatgg
     2401 gcggcggcac cgggagatcc ctgatttcct taatgagccg ctgtatttcc tcagctttta
     2461 ccatgcgccc gcgtgtgatg gaggccattt cctccattag cgcggtattg acggggctgc
     2521 ctattttttc cagcgtttca ctcatggtga tgacgggaag cgtaacggat ttatccggtt
     2581 ccgcggagga tgaagctgtg agcttccagg tgccgggacg gttgattttg acggaggcgg
     2641 taaaggagcc ccatgtatgg tccatagtgt ccatttgaac gcggctggag cttccttccg
     2701 gagttgtaat gtccagaaaa acctctccat tctggagagg ggcccccagt ttgtcggaga
     2761 ccatggcggt gacggtcagc gtgtctcccg gccggggccg ttccggggtg ggaatcagac
     2821 ggattctttc ccctgcggcc atgttgcgct ggtaactcat ccagcgggcc acctggctcc
     2881 agaaacggta atggtatttg tcttctactc ctcgcctcca tcgccaggcg gaatccgttc
     2941 ccatgaagag gacttttccg ttgccgcagg actgggtgac aataagagga atgcggccat
     3001 aggcattctt atccgccgca tgcacggcca ggacagtgct tcccgctttg ggccgttcca
     3061 cgggggcgta ccagttgaag cccggcagat tgcgccatac ttcttcgttt tcaccctccg
     3121 tatccgccag catggtaagc agggagccgc ggccttccgg cgtcaggata aggggggatg
     3181 gggaaggctg ggttgtcccc tccggcttgg tgttgaggaa tgtaacgggc atcaggtccc
     3241 ccagttccga tttcaggagt tccatttgcc tgccctggta gccggggagg aagacgatgc
     3301 cgctggcctg gttttttacc atgcctttga gcagggaggc ctgttcttcc gtcagccctt
     3361 tggagcccag ccccacgtcg ccgatgaaga tgacgtcata ccgggccagg tcttccatcc
     3421 tgtccgggaa tttgaccagg tacccggggc cttcccccat ttcttccagt tccggatgaa
     3481 agagcagggt atgcacgtcc acgccgggat cccggtacag ggcgttccgg atgaagcggt
     3541 attcccagcg cggtagggta tcgatgacca gtgctttgat ggattccctc ctgccggaga
     3601 tgctgaagga agaggcgtta ttattgtgca tccgttcctg gggctggacc gggagtttga
     3661 gttccagcgt ttccgcccct tctttttcta ttttccacag aaccgcgtcg gatacctccc
     3721 cttgggccgg aatggtgacc gtgcgggtga cggtttttcc tgtatccctg gaagtcatga
     3781 tgagcgtggt gcgcgtttcc ttgcccagcg tgcttttaat ggtaaagggt atttgcaccg
     3841 tttccccgat gatgccgtaa gtgggagctt tcacgtcctg aagggccagg tccgggaggg
     3901 gatacgggct gcctgcggaa atgatgaaca gggggatgcc cctggtgcgc atgcgctggg
     3961 cctgcgtcag cacggaggat gaggcgttgt ggctgccgtc cgtgaacatg atgaccgccc
     4021 ggaggttgtc ggaggattgc atggcgtcct ccagcggctt ggccaggtct gtgccggcca
     4081 tggcataatc cggagagtcc ggcgcaggtg gcgcgccgaa ggaggcgtat tccactacat
     4141 gtgtgtcttt aagggattcc gtactgttgc ccttgagcag ttcctgcacg taggccgtgc
     4201 gtgtgacgac atgctggggg gagatttcaa catcctgcgt ttccatggag ccggagcggt
     4261 cctccagaat ggaaaccttg ggtttttcct gcggggcgga ggtgatgatc cattccggct
     4321 ggaagagcag gaagcaaatg aagccaagga ttgccaggcg aagaatttcc agtttgaatg
     4381 tcagcggccg gcggttgcgc ttgcaggaga tatagctcat ccagatgccg gccgccataa
     4441 ccagggcggt caggatgacg cttgacgcag taggttgaag gctcaggttc atggacgggt
     4501 ggtgggggag gggcgtttgg ccgtctggcc gggcaggcac aggagggctt ccatcagcag
     4561 gcaggcgagg gcgaagaaca ggaagagctt ccatgcttcc tgcaccaggg agggggcttc
     4621 ctctgcactc tgcatggaac tgatggaagc ttccggcaga aggaggtgta gtttttcatc
     4681 cgtaatctgg tcaggcatgt cttccggcca ggggcggttc acggcgtagg tctgtgctcc
     4741 cagtcggtac acgcccgccg tatcggccgg agaaccggag gggtggcttc cctgggcatt
     4801 atccaggcgc acgggggtgt ccgtggcgga ttgcggcagg gaggcatcat tcacccgtag
     4861 ggaaatggag gtggaaaagc gttccgctcc ccggtccgcc atgcgctgga gcagggggag
     4921 cagcaggtgg ccgtccgcca ggttggacca ggaatatctg ggcagcgttc ccaggaacag
     4981 agcggccccc gttccggccc tgacctgggc cagagcgcag gaaccgtcat cccaggaggc
     5041 cagagtgcgg tatttgccca gcaggggttt tctacgcacg gcgcggagac ggttggcggg
     5101 gatgggtgtc tgatcggaac cgtcgcggag gaatcccctt tgccggtccc acgtttccag
     5161 acggtagtat tgttcggacg gagccgtctc cgtcgcgccc caggaaacgc ccaggaatga
     5221 atgccgtgtt ccgtgttccg tgtcgtcagg caggaaaagg gctattcctc cttcttcaat
     5281 gaatgtctgg agtttttttc ccatttccgc gcgcggcggc ggcccgtacc agacgaccat
     5341 ggactgggag gccagattag cctgagcttc ccgcgtattg ccgggcatcg tggctttcct
     5401 gccgggcagg ccgggagggg cggacatcag ggaaagggtt tttcccactt ctcccgaagg
     5461 accgaaaatc aggatgttgg cgagaggctt gggggcgaag gtgaagaagg aaatgtcatc
     5521 cctgatgaag tcgtcatgag gcagcgcaac gaagccgaag ccggattcct tgccctgcgg
     5581 caggggtatt tctttccgga tggaggtgtt ttcccctgcc agttgcagcg tggcggagga
     5641 ggaggcgtta tccacggaaa gctgcaccgg cacgtcctcc ggctggttgg ggttgaaacc
     5701 ctgccgcagc acttccaggt ccagcaccag tttcccgtta tttacctgag cctgccggac
     5761 tctgatgccg cggttgcttt ccggcctgtc gcggagggcc atgatgcgga tggaaggggg
     5821 caggggaagg gcggccagcc tctggcgcac accgggccag agaggggagt ctggcttcca
     5881 ggaagaggcc tgcatgtccg aggcaatcca gatttccgtc tttcctgcgc cggattcttc
     5941 caggtacggg atggcttttt gcagaagtgc cggaatatcc gcgcctccat ccgttgtctt
     6001 gcttacagtc agatccgcca gggcttccgg agcgggaatc tgggtcacgg ttccggtggc
     6061 ggaatcaagc agggacaggc ggcagtggcc gagctgcgcg aacgtggatt ccaccagcgg
     6121 ggggatggca tcccggaggg agctggtttt gtccggacgg gcatccatgg aggcggaacg
     6181 atccagaacc agcaggactt cattcaatcc ggagctcccc cagccgaaaa agcttccgag
     6241 caggggccgc gcaaaggccg ttaccagtgc cgccacggcc atcacgcgca gtgccagaat
     6301 cagatagtgc agcagttttt tcgttccacg tgtttccctg gccgcacgga gaaggaattc
     6361 catggccgcc caggggacgg aacggtgccg ccaccggttg accaggtgga tgatgatggg
     6421 aacggaggct gcaaagagag cccacagcag ccagggagag ttgaaggaca tggcgggaca
     6481 gggataattg gagggtcaga gctgcctggt caggtggagg gcgggccggg cagcagttgc
     6541 cggaggatgt gaagggtcat ttccccggtt tgctggaagg atttttcgcc cagaatgccg
     6601 atgttatcct tggaggtgtg ccaccagttg ccgtcctgga agtcgtcaat caggttcagc
     6661 gtgtccacgc cgcgctccat gaaaggaacg tggtcatcca ggatggcata gccggatact
     6721 ccccattcct ccggggaata acccagttca cggatagcgc gggaataaac ccggtacatg
     6781 gattggggcg tcatggccgg gatgcgtatc tttttaccct gccgccccac catgtccaaa
     6841 ttgatcatcc attcgggaag cggctgccgc atggaggatg cgtaatgcct ggagccgtac
     6901 aggccgtcgt cgctgtccat atgttccgca aaactttctt ccccgtcaaa aaacacaagc
     6961 tccaaatttc cggcgcgtgc ggggtctccg gacagaatgc gggctgtctc cagaatggcc
     7021 gcagctcccg atgccccgtc attggcgccg gtgaatccgt cgatgccccg cttggtgtca
     7081 atgtggcagg tcagcaggcc ccgaaccgga tccaggaagt ttggagcctt gccaaaacgg
     7141 gccctcaggt tgacgaaccg gacggggccc tggggcgttt ccttttcaaa agcctgttcc
     7201 cggcaggtcc agccgcattt ggccagttcc tccttcaggt agtccatctg cctgcggtag
     7261 ccgggagtgc ctgaagcacg attccccatt tccgttatcc gcgcggcgtg gaacagagcg
     7321 tttcctccgt tgaaacaatt cgtttgccgg atttgcgcgg gaacggtttc cttaccgggc
     7381 acgggcgttt ccacggggtt ttcgcactgc atcagcaggg ccgtgaacag gagaagtccg
     7441 atgcggggcg tcatggaaaa agggggggga agtttacttg gaatcaggcc attcatcctg
     7501 gcccagaagc tcaaggatgc ggatgaattc cgctttgctg gagaaggtga tttcaatggc
     7561 cccccggctt ccctggccgg aaatgttcac cggagtaccg aattgtttgg cgaggatgct
     7621 ggaaattttc ttatattgag gggaggacgg ttttttaacg ggagccggct ccggcgggtt
     7681 gagcatcttc tgaatagcct tctccgtctg gcggacggtg tagcccttgt tgacgatgtc
     7741 gcgcccaagc tggatttgct ggtccttgtt tttgagggag agaagtacct tggcatgccc
     7801 tacgctgata aaggtattgc ccagcatgtc ctgaaccggt tgaggcaggt cgagcagccg
     7861 catgctgttg gccacggcgg cgcgggattt gcctacgcgc ttggcgatgt ccccctgctt
     7921 catgcggaac tccgttttca ggcgcatgta gccggcggct tcttccagcg ggctgaggtt
     7981 ttctcgctga agattttcaa tcagggccag ttccagcacg tccttatcgg aagcttcccg
     8041 gatgatagcg ggcacggtgg acagccccag gatgccggag gctctccagc gcctttcccc
     8101 ggcaatcagt tcatatttgt cgctttgcgt tttacggacg attaaaggct ggatgatgcc
     8161 gtgttccttg atggaatcta caagttccgc cagttgttcc ggcgtgaaaa tggcgcgcgg
     8221 ctgcagggag ctgggaagaa tggcggcatg ggatagttga agcacaacgt ctcccgcctg
     8281 gggag
//
LOCUS       DAWEMX010000114         8220 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3574_length_8220_cov_11.220086, whole genome shotgun sequence.
ACCESSION   DAWEMX010000114 DAWEMX010000000
VERSION     DAWEMX010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_8220_cov_11.220086"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2106..2115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1864 a         2575 c         2170 g         1601 t
ORIGIN      
        1 ggcggcacgg attatatttg cccggcggat cttccggaag agaccgccgc caacgtgcag
       61 gaggcggccc tgaaagccta caaggccgcc ggcgtggaag tgtacggccg cgtggacgta
      121 ctgctgcgcg aggaagacgg cgcccccttc gtgctggaaa tcaacaccat tcccggcatg
      181 accccttcca gcctgcttcc caaggcggcg gcggcctccg gatggcccta tgaagacctc
      241 tgcgaaaaaa tagcggaatt atcgctagcc accccccgta aataacgtaa tatctgacag
      301 agggcgggga gtcgcccctc ccgcaccccc cgatgtcgca caaagccaca acgccagtcc
      361 cccgttccgc caaaaggccg gaaatgcttc ttctggaacg ggaggccaga aaagagacca
      421 tcgaacgcaa gggcaaaaac taccgttttt ggctcttccg gcgcaagctg taccacattg
      481 tcaccgttta cgcgcttatt ttcggcttaa tcggggctat cgtttttctc tggaaagatt
      541 acatcctcaa atacgactgg ctttccatcg acaccgtcac cctgaaaagc aacggcattt
      601 tcaactccga acaggccttt tccgttatgg gcatagggcc gcaggacaac attttttcca
      661 tagacgccgc ggagctggag cagcgcctga aaaaatgtcc cgccatccgc cgggcttccg
      721 tcaaacgcca gatatcctcc aaccccacgc tgctggtgga cattgacgcg cgcatccccg
      781 tggcctggat tgactgcccg gaactgggaa ttcacccggg agatgccacg tacggcgctt
      841 tggcagacaa ggaaggcgtc atcttcccct gcatggagca ggtccacatg ccttatatcc
      901 gggaaaagcg catgccctcc gtcacgctca ggccgcccag ttccggcaaa ctcagctacg
      961 gcgtcagcat ccgggaactg gaagcgccca tgaaactgat tgaactgctt tccggcacgg
     1021 taacggaata ccttcccagc atcgtctcca taacgacgcc caacgactgg tctttctgtg
     1081 tgcgttttac caatgactgc caggctactt tcagccatta cggcctggaa caccaggtgg
     1141 aaaaactgtc ccgcgccctc cggcacgcac gccagacgca ccgcaaaatc agttccatca
     1201 acctgattcc ggagcacaac attcccgtca tctttgacga ttcctacgaa gatattcctc
     1261 ttgcggagcc gattgaagaa taacgccgcc cagacattct ccggaaaagc cgtgttcttc
     1321 tccgactcca aacttctttt ccggtctcgt tccccctgtt tcccctaatt tcccgccgaa
     1381 cggaacaccc ggttttcaca agattcttcc catgtcagaa ttctgttctt tgtcaatgag
     1441 gaatgagtct gcgaagaccc acagaacact tgccaataag gctgtcccgg ttatactgcg
     1501 ctacgatata tgagcaacga aacaccttta tcccccgaag cggaaaaact ggcggcagcc
     1561 cgcaagcgca accttgacct ggccctctcc caaatccaga aagacttcgg agaaaacgcc
     1621 attatgcgcc ttggagacaa cgtcaaaatg gaagtggacg tcattcccac gggcaacctt
     1681 ctgattgacc gcgctctggg tgtgggcggc tttgcccgcg gccgcattgt ggaaatctac
     1741 ggaccggaat cctccggtaa aaccaccctg accctgacgg ccatcgccca ggcccagaaa
     1801 tccggaggcc tggctgcatt cattgacgtg gaacacgcac tggaccctca gtatgccgcc
     1861 cgcctgggcg tgaacctgga cgacctgctg gtttcccagc caagttccgg tgaagaagcc
     1921 ctgcaaatct gcgaggctct tgtccgctcc aatgccattg acgtcattgt ggtggactcc
     1981 gtggccgcac tggtcaccaa gcaggagctg gaaggagaaa tcggggattc cacggtagga
     2041 gcccaggccc gtttgatgtc cgccgccctc cgcaagctca ccagcttcat ttccaaagcc
     2101 cgtacnnnnn nnnnngtacc gtctgtatct tcaccaacca gatccgtgaa aaaatcggcg
     2161 tcatgttcgg caatccggaa accactcccg gcggccgcgc cctgaaattc tacgcctccg
     2221 tacgtgtgga catccgccgc atcggccaga tcaagggcag cgacggcgtc atagccggga
     2281 accgcaccaa aatcaaggta gtcaaaaaca aagtggctcc tcccttcacg gaatgcgaat
     2341 tcgacatcat gtacaatgaa ggcatctcct ccgtaggcag tctgctggat ctggcaacgg
     2401 aatatgacat cattcagaag cgcggctcct ggatcagcta taacggcagc cagatcgccc
     2461 agggccgcga cgccgccaag gaagctctca aatccaaccc ggagctgtac gcggaaattg
     2521 aagaacaggt caaggccaaa atggacgaga agaccgccaa ataatcccgc ctgatcccgt
     2581 tccttcactg ccatttcagg gcggctcccc ttcccgggag ccgctttttt ctctccggaa
     2641 acagaagcgc aaaagaatct ttcacggaaa cttttccctt gtgcgtcatg aaaaatccgt
     2701 tagtctggat gcgatatcgt cctatgtatc cgacctctcg cacatcattt gcagccgtgc
     2761 tcggcattgt gggcatgttt ctgctgacgt tgtccacggc cttcggatgg aatccggaaa
     2821 tcattaatgg agtgcagtat attccgatgt ccgaagtgcg cacccattac aaactgacca
     2881 gggaacgcac ggaagggcgg cagaaagttt acgaggtgcc ggaaaagatt caaatccgta
     2941 tccaggcgcg cagccaggag atgttcatga acaacatgaa gttcgtgcta tcctatcctg
     3001 tggctgacca tccctccaag gggctgatgg tttccaatat ggacctgcac aaaatcattg
     3061 acccggttct gcgccccact tacatcgcca accgccgcag tttcaacaca gtagtcattg
     3121 atcccggcca cggcggccat gacagcggca cgcgcaaccg catcagcagg gaagcggaca
     3181 tcaacctttc cgtgggcaaa aagctgcgcg accgcctcaa ggccatgggc taccaggtgg
     3241 tcatgacccg tgacacggac aacttcattg cccttcagga ccgcgtccgc attgccaaca
     3301 ggcacaataa tgccatcttc atcagcatcc acttcaatga cggaggctct tctgcccgcg
     3361 gggtagaaac cttcacgctg gcgcccgcgg gcacttcctc ctccatgtcc cgcaatatcc
     3421 gccacgacgc cctgcagggc aacgcccagg acagcatgaa cattgccctg gctacagccg
     3481 tccaggggca catgctgaaa ggaccgcttg ccatcaagga gggcatttcc atggttgacc
     3541 gcggcatcaa gcgcgcgcgc tattccgtgc tgtgcaccat caagcatccc gccattctgg
     3601 tggaaggcgg gttcatgtcc aacccgcagg aggccctgct catcgccacg gagcgttatc
     3661 agaatttcat ggcctcttcc ctggccgccg ccgtgcacca gtaccgcacc gccctgggcc
     3721 agcaagtgcg cagaacgcgc tgagttccct aactgtcccg gggcctccct tcagcagcat
     3781 gaacatcatc cgcattcccg gcctggcaga ttatcaggaa accttgaggc tccaggaaca
     3841 actggtccgg gcccatcagg aagacccgga cagggaaaac gaactgttgc tgctggaaca
     3901 tgccggggtt tataccatcg ggcgtacacg gaaccacgcc agcctgcacc ccggttccgt
     3961 gcttcccttt cccgttcatg aaatcaaccg cggcggccag gccacctacc acggccccgg
     4021 ccagctggtc ggatacccca ttacggacct gaaccgctgc ggcagggatc tgcaccgtta
     4081 cgtcaccacc ctggaaaaca ccctgattga aacctgtgcc cggttcggcg tgcaggcccg
     4141 catccgggaa gggctggtag gggtctgggt ggaaaaccgt aaaatagcct ccatcggcgt
     4201 cggggtgaaa aagtggatcg ccatgcacgg cttcgccctc aatataaccc ggaaatccct
     4261 gcctcccttc cttgctatta ccccctgcgg catccagggc gtgcaaatga cctgcctgga
     4321 ggatgaaatg tcctcccccc tgccggaagg aaacctgttg aaatgtttcg gagacgtttt
     4381 tgcagcacta tgggaagaaa aatttaaccg gtcttaaggg gattcggaac gtgcggaggg
     4441 catgaaaacc atcctttcct tgaaggcgga aaggccccaa aggctggaaa gaggcccatt
     4501 cggaccatga atgcagagga aatgttcaaa aaaacgcctt atgtaagaca cagaccgtgt
     4561 cagcaatacg tccttgcatc ttgacgcggt tcccgaacgg acggatgctt atgtcagtta
     4621 tggggaaaac gcttttccaa aaaatctggg acgctcatac cgtcggcatc ctgccggatg
     4681 ggagaacgca aatgttcatc gctacgcacc tgctgcatga agtcacctct ccacaggctt
     4741 tcggaatggt ccgggacctg ggcctgaccg tgcgccaccc ggaacgcacc tttgccactg
     4801 tggaccacat cattcccaca gacaaccagg cggaaccgtt cgcggacgcc acggctgacg
     4861 ccatgatcag ggaactgcgc cggaactgcg ctgaaaacgg catccgcttt ttcgacctcc
     4921 ctaccgggct ccagggcatc gtgcatatgg tagggccgga actcggcatc actcagccgg
     4981 gcatgactat cgtatgcgga gactcccata cggccaccca cggagccttc ggctccattg
     5041 ccatgggcat cggcaccacg caggtgcgcg atgtgctggc tacgcagacc ctggccctca
     5101 gcccgctcaa ggtgcgccgc atcaatgtga acggaaagct ggcccccggc gtgcgcgcca
     5161 aggatgtggc cctgcacatc atcgggcttc tgggagccaa gggcggcctg ggcttcgcct
     5221 acgaatacgg aggcgaggtc attgacgcca tgagcatgga cgaacgcatg accctctgca
     5281 acatgtccat tgaaggcgcg gcgcgctgcg gttacgtgaa ccctgaccgg accacggtgg
     5341 aatacatcaa aggacgcctg ttcgccccca ccggcacgga ctgggacaag gccgtggaac
     5401 gctggctggg ctttgcttcc gacgcagatg cggaatatga tgaaatcgtg gaaattgacg
     5461 gggcttccat tgagcctaca ttgacatggg gcatttctcc ggaccagaat acgggcatca
     5521 gcggcagcac gcccaaccca tccgacgcag cggacgacga tgaacggaag atgatcaatg
     5581 aagcgctgga atacatgaaa ttccccgcga atatgcctct taaggggctg ccggttcaag
     5641 tgtgcttcgt aggttcctgc accaatgggc gcatttcaga cttccgggaa gtggccgccc
     5701 tcatcaaggg tcgccatgtg gcccccggca tcagggctct ggccgttccc ggctcccaga
     5761 tgactgcccg gcagtgtgaa gaggaaggca tcgcggacat tttccgtgaa gccggctttg
     5821 aatggcgcct ggcgggttgt tccatgtgcc tggccatgaa tccggacaag ctccagggtg
     5881 accagctctg cgccagttcc tccaaccgga acttcaaggg ccgacaggga agccccaccg
     5941 ggcgcaccct gctgatgagc ccggccatgg tggccgccgc agctctgacc gggaaagtct
     6001 ccgatgcccg cgaagtgttc tccctgaatt aactttccaa ttttttcctt tatgtctctg
     6061 acccaattca cctccatcat cggcacctgc gttcccgttc cgggaccgga catggacacg
     6121 gaccgcatca tccctgcccg cttccttaaa tgcatcacgt ttgacgaact ggcgggagtc
     6181 atgttctggg acgaacgctt cgacgaaaac ggacaatcca aatcccaccc ggtggacgat
     6241 ccccgcttca aaggggcttc catcattttg ggcggctcca acttcggctg cggctcctcc
     6301 cgtgagcatg ccccgcagac gatcaggcgc tccggcatca aagccgtcat tgcggaatct
     6361 ttcgcggaaa tctttttcgg caacagcacc ggcatcggtt tgccctgcgt gtgcgcgtct
     6421 gccgccgaca ttgccgctct ggcccggcac atttccgatc acccggaaac ggaagtgacg
     6481 attgacctcc tgaacatgac agcttcctgg aaggaaggct ccttccccat ccggatgccg
     6541 gcggaagccc gcgaagccct gacccggggc cgctgggatt ccattgcgga actgcttgtc
     6601 aacatggatg ccgtagaaaa gaaaaacaag gaactgcccc aggtcgcagc ctcctgctga
     6661 ttcagcattc acctctttcc tccccttcta tcccccttcc ccatatgatt ttcagaatta
     6721 cagactacgt ccactacggc acgctggaca accgtgaacg cggaaccgtc cgccttacgc
     6781 tgcagctcat ggggatgccc catccggtaa atatcaccct gcagggggac tgcctccagg
     6841 atctggccgg ttgcgtggtt gatttcagga atccctctcc ccagactctt cccgcggaac
     6901 ttacccagat cccggaacat atccagggag tggcgggaga catgacggcc agccgccgca
     6961 tgcctgtcaa aggcaggaaa accatggaaa acgccctgta cctggaatgg ttcaccagcc
     7021 accatgacat ggtgctgctg gaaagtacgg tcttctccat caaagtctcc ctccccgaat
     7081 ggattatgga ttcctgcgaa gagcaggccc agatcatggc gaaccagcag atgctgcgga
     7141 cccaggtaaa ggaatggtcc agggcctatt ccaaccacca ggaggacgga agcctgcccg
     7201 accaccactg ggacaaacgc ctgcgggagg cggaagccat cgccatcgcc tatcaggagg
     7261 tattccagaa atacaggctg aaccccagcg gagacatccg tgtggccttt gtcatgggct
     7321 gggatgaagt gctggacaac atcgcccagt cggaagaaac ggggacgccc tgttcctgca
     7381 aaagcaccgg catgctcagc ctgtttgaca ttctcaacga acaggaggcc cgggaagtgc
     7441 agtcctgcat gtttcatccc ctcttccagc aggtgatgga gctgacggac ctgtgccagc
     7501 accggttcag ccgggaaatc aacaaagccc agagaaacag gacggggccg ccggaacccc
     7561 tgaaccagat tttttactgc atccgctaca tcaccccgcg cattctttcc tgcctccttc
     7621 aggagaagga agacgcggcg gactactgca ccatggccgc ccgcatggcc ctgtgcgtgg
     7681 aacaaacgcg ccagacggta gctgcgctgg acagccgcgg ctaccagatg gacggcgaaa
     7741 tagcggaacg tttctcatcc ctgctggagg aagtcaattc cttccaggaa agcctggcaa
     7801 cacaaagccg caaaagcaac ctgtaacaac aaaaccttcc ctggcaacac aatcttcatc
     7861 tttgcgtatg atacattccc acacctgttt tcaagggctg tgcgcagtcc tggccggtgc
     7921 cgctctttcc cacgcggatg agccggctgt ttccatttcg gacttcgggg tggacaaggt
     7981 catgtccggc cctgtcgttt ccatcaatga ccagtacaag ggcgtcgtga aaattgagat
     8041 ggattccctg actccggatt acgctactcc gtggaatacc ggccgctacc aggggggcat
     8101 cggcaccggc tttctgatcg gggaaaacgc cttcatgacg aacgcccatg tagtcagcaa
     8161 cgcggaacgc atttatatct ccatgtacgg agattcccgt aaaattcccg ccagggtcaa
//
LOCUS       DAWEMX010000115         8127 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3634_length_8127_cov_10.598612, whole genome shotgun sequence.
ACCESSION   DAWEMX010000115 DAWEMX010000000
VERSION     DAWEMX010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_8127_cov_10.598612"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    917..926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2316..2325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1608 a         2397 c         2310 g         1792 t
ORIGIN      
        1 gcacacagca agacgcagga acatgaaaaa agacgcagaa aggaaatata atcacgcatg
       61 gcatacggtt ttaaaataat acgccaggga aattcttttt cattcaatag gaagactact
      121 ttacggcatt ttctctcaaa cgtcagacaa taaatgaata acttaattga ttcggatttc
      181 tagaccggaa gccaaacgtg aatttcaaaa gaccccggct gaaatcatcg cgggagtgga
      241 agtggaaccg ggaggaatga aactccatga agtggtccgt ccgctcaacg acatcgccgt
      301 ccgttatgaa gtggcagccc tcacgatagc cgagccgatg ccgcgcctgg cgatcaggat
      361 cagaaacatg ctcagccagc tccctctgct gaagccggac gccccgccgg cccagccggg
      421 caggaaacgg aaaaattaaa atcatcctgc cgtcgattcc cggtctttgg aaaaagcagg
      481 cagaggaaga aagggacaac cggacattca ggcatcctcc agcatccggg ggcgcagcag
      541 gtacaccttg tcccggcggc gcacccgttc gggaaccgcc agatagacgg tttctccctt
      601 ttgagcggct tctacgggct ccatgccctc tgccgtctcc ctccgcatgg ctgccacttc
      661 catgcggacg gctcccgtag tggggcctgt tatgaggatg gtctgccccg cggacaggcc
      721 gccggcttcc aggaacaggg ccgccacccc gttcttctta taaaagttct ctaccctggc
      781 aatgtggatc ttcaacaggg cagcgcggct gttggcggaa ccgctccatt ccccccactt
      841 cacgcccagg taatagccgc cttgccagaa tccccggttg aaaacggatt ccagccgttt
      901 catccacgct tccgccnnnn nnnnnncttc cgccctgtcc gcggaaaagg ttccgtcctg
      961 gcatgcccgc gcggcttccc ggtacacgga ggtaaccgtc ctgacgtaat ccgaggaacg
     1021 gccgcgccct tccagcttca gcacggaaac gcccgcgtcc agaagctggt ccagcaccgg
     1081 aatggtacac aggtccttgg gagacatcac gtatttgttg tctatcacca gttcattgcc
     1141 cgtttcctca tccgtcacgc ggtaggcgcg gcggcagttc tggaagcagg ccccccggtt
     1201 ggcggaggag ttgtaggccg ccaggctcat gtggcatttg ccggacacgg ccacacataa
     1261 cgccccatgg gcgaaaattt ccacccgcag cagctccccg gaggggcctt tcacgccctc
     1321 cttccggatg gattcaatga tatgcctgat ttgcgccagg gtgagttccc gggccagcac
     1381 caccacatcc gcgtactgcg cgtaaaattt gacggaggcc atgttgcaga cgttggcctg
     1441 cacggacata tggacttcca gcccaatgga acgcgcataa gaaatcaccg ccaggtcgga
     1501 agcgatgacg gcatccacac cggctttccg ggcggcgtcg cacagggcat gcaccgcctc
     1561 cagctcttca tcgtacacaa tgatattcag tgtcagataa gctttggcgc ccgcctcatg
     1621 gcagcgctcg acgatttccg gcaaatcttc ttccgaaaaa ttgaccgtgg cgcgggaacg
     1681 catgttcagc tttcctactc cgaagtacac ggagtccgct ccgccctgca aggcggccgc
     1741 cagagattca aacgaccccg ccggggccat gatttctatc cggttttgaa aaggcatggg
     1801 tcaaagtcct ccgggttctt ccatcagaat gacggaaatt cttcccttgg gaaagcattt
     1861 aacaatggac atgtgaatat tgcacaggcg gtccggggaa ttgcaatccg cgcagattcc
     1921 ggtgtgcatg cagggagtcc tgaacccttc atgccgcctg gcgttcaggg gcgcaatgcg
     1981 gcggatgcgt tcccgggcct ctgccggcgt ggccaccagc ttgttggaac cggccagcaa
     2041 aatgacatgc cggggtccga atgccaccgg agccacacgg ttgcccacca tgtccaccca
     2101 gtgcagggtt ccttccatgc tgacggcatt gacgccggtt aaaaacaggt ccgccgtcat
     2161 gccctggcgg cggatttccc atttctcctc ccgcaccatg cccggaacaa agccgtcata
     2221 aaactgaatg tccggattgg agcgcaggtc atcaagaatg cccgcagcct tcagggtcat
     2281 ggaatcccca taggaagcgg acgacggcct gaactnnnnn nnnnnggccc ggcactacaa
     2341 aactccggaa tccacgccgt tccagacgtc cggcacattg ctccaatgaa agggggtctt
     2401 ccataaaaac ggcccatgaa gccccctgct ccggcatggc gccggagcag gaaaacctgg
     2461 atgttattct tccgtcggag cttccatttc accggacact tccccggctc ccgcgtcttc
     2521 cgtttccgcc ggggcttctt catcttcatc atcgggaatg acgatggaaa tgtcctggat
     2581 ggattcgccc tcattcagcg tcatgagctt cacgccctgg gtggcgcggc ccgtttcacg
     2641 cacggtcgcc accttgatgc ggacggattg tccggccgtg gtcatcagca tcagctcgtc
     2701 ctcgtcggac acgaccgtgg cggaaaccac cttgccggtc ttatccgtgc agttcatggt
     2761 cttgatgccg gtgccgccgc ggccctgcgt gcggtattca tcaaaggaag tgcgcttgcc
     2821 gaggccgttt tcagaagcca ccagcagggt tttctgcggg tccacaatgg ccagggccac
     2881 aacgtagtcc cccgcccgcg ggcggatgcc gcgaacgccg atggtgttgc ggccctgcgt
     2941 acgcagatct tcttccggga agcggatgct catgccgtca tgcgtgacga gcacgatttc
     3001 atcggccccg ctggtaagtt ccgcgttcac cagactgttg ccttcctcca gattaatggc
     3061 aatgatgccg gccttgcggt agttggcgaa atcgttcagg ctggttttct tcaccgtgcc
     3121 gtccttcgtg gcgaaaacaa cattgccgct gccttccgca aaggtaatgt cattgccttt
     3181 ttcatccacc cggcgttcca ggcgcaggat ggcagcgatg gcttcttccg gctggagatc
     3241 cagcaggttc ttgatattgc ggccctgcgc gctgcgcgcc gcttcgtcaa tctcatacac
     3301 gcggtccacg tacacgcgcc cggtgttcgt aaagaacatc aggtagtcgt ggttctgggc
     3361 ggcgaacagg tgttcgataa agtccgcttc cgcatccttt ttggctcccc tggtagtggc
     3421 agccttcacg cccttgcccc cgcgcgcctg cacgcggtat tccgcggagt tggtgcgctt
     3481 gatgtagccg ctgtgcgtga tggtgacgat catcgtgtca ttcgggatga gatcctcaat
     3541 ggccatatcc ccggagaagg gaatgatgcg cgtgacgcgc ggcgtggcgt acttgtcctt
     3601 aatcgccttc agttcgtcct ttacaatgcc cagcacgcgg gattcgttcg ccagaatatc
     3661 caggtagtcc ttgatgcgga ccagcagctc cgcgtattcc ccggaaatct tgtcgttttc
     3721 caaggcggtc aactggtaca ggcgcaggtc caggatggaa ttcacctggc gttccgtgaa
     3781 gacgtagcgg tccccctgga tggaggcctg ggaacggatc aggatgccca ggctttccgc
     3841 cgtctgcgtg gagaatgtgt aattctgcag gcgttcacgg gcttcatccc ggtttttgga
     3901 atcccggata atggaaatga agtcatccat gtggtgcagg gccagcagga aggcttccag
     3961 gcgctcggca tccttctccg catcccccag cagatagcgc gtgcggcgca cgaccacctc
     4021 gcggcggtgc tcgatgtaga agttgatggc gtccatcatg gcgagcgtct tggggcggtt
     4081 gtcatgaatc gccagcatat tcaccgcaaa ggaagtttcc atggaggtga gcttgtaaag
     4141 ctggttcatg accacctgcg ggcgggcatc ccgcttcagc tcgatttcaa tgcaggtttt
     4201 ctcatcggaa aggtcacgga tgccggaaat atccgtcagg attttatcct tgtacagttc
     4261 cgcaatgcgt tcctgaaggg cggcgcggtt cacgccatac ggcacttccc ggatgatgag
     4321 aagatccttg ccggaatccg ttacttccat gtctatcttg ccgcggatgc gcacggagcc
     4381 gcgcccggtg cggaagtagt tgtcaatgcc tgaaaagccc agcacgtcgc ccccggtggg
     4441 aaaatccggc cccttgatgt actggcgcag ctcttccaca gtcatgtggg gattgtcaat
     4501 cacggcgcag atgccgtcca ccacttcccc caggttgtgc ggaggcatgt tggtggccat
     4561 ccccacggca atccccgtgc cgccgttcac cagaaggttt gggaaagcgg aggggaacac
     4621 ggtgggttcc gtgtgttcat tgtcgtatgt gggaacaaaa tccacggtgt ccttgtccaa
     4681 atccgccatc agggccacgc ccatgccgga gagcttggct tccgtgtatc gggcggcggc
     4741 gggggggtct ccgtccggcg tgccgaagtt gccctggccg atcacgagaa tatcccgcat
     4801 ggaccagtcc tgcgccatgt tcaccagggt gccgtaaatg gcggcatccc cgtgagggtg
     4861 gtagttaccc atcgtgtcgc ccacgatttt ggagcatttg cgggtcttgc ccccagggga
     4921 cagattgagt tccttcatgg cgtacaggat gcgccgctgg gaaggcttga ggccgtctct
     4981 cacgtccggc agggcgcggg agatgatgac ggacatggag tagtccagga aagaagtgga
     5041 gagctcactg gctacatcca ccggtttgat tctattattt tccatggtgc tgaaagagag
     5101 tgcgggggtt aaatgtcaag gttgcggaca ttgagggcat ggtccacgat gtaattcttg
     5161 cggggttcca ccacatcccc catcaggatg gtgaacattt catcggccct gacagcgttg
     5221 tcatcgttca ggatgacgcg gagcagctcg cgccgttccg ggtccatcgt cgtcttgaac
     5281 aagtccttgg catccatttc acccaaccct ttgaatcgcg tgatttccac cccgcgcttg
     5341 ccgatggaga tgacggattc cagaatgtcc atgacggaga acaggggagt tactttttcc
     5401 ttatcccctt caccctccac cagttcaaac aggggcgtat ccatggagac aatcatattc
     5461 cccggaatgc ccagttccgc cagccgggcc agggcccgcg tgatggcttt ggattcatgc
     5521 agttcatgag tgatggagcg gcggctgggc ccctcccgtt ccggaagcgg attttccagc
     5581 aactcctctt ccgagggcgt gccgaagatg aaaaggtccc tgttttcctc agaaaaggcg
     5641 gtcagggcct ccatgtcatg gaagaagagt atttcctctt cattgccgca gcgcaccttc
     5701 accaggaatt ccgggaattc cccgttggcc tcccgatggc tgagcaggtc ttccagggaa
     5761 ccgccctgcg cctgcatgga ttccgtatag cgctggaggt tgaccagcgt ttccagaatg
     5821 gcggagagtt cttcgggaga gaaggaacgg gagccgtctg cgaaacgcag ggtcacgtca
     5881 ttaacggcca gttcaatcag tttgtggttc agggccacgt catcctgcac gtactcttcc
     5941 ttttttctgc ggatgatgcg gtacaggggc gcctgagcga tgtacagata cccggcgcgc
     6001 accagctgcg gcatgtggcg gcagaagaaa gtgagaagga gggtgcggat gtgggcgccg
     6061 tccacgtcgg catcagtcat gatgatgatt ttgtggtagc gcacgcggtc cagcttgaag
     6121 gaggcttcat cgtccccttc cccgattccg gcgccgatgg ccgtaatcat gttctggatt
     6181 tccttgctcc ccagggcttt gtccaggcgg gccttttcca cgttcagcac tttcccgcgc
     6241 aggggcagga tagcctgtgt gcggcggtcg cgccccatct tggcggaacc gcctgcggag
     6301 tcaccttcca caatgaacag ctcgcacttc gcgggatccc ggtcggaaca atccgccagt
     6361 ttgccgggga ggccgccgat ggagagggcg ctcttgcgga ctgtttcacg cgccttgcgg
     6421 gcggcttccc gggcacgagc ggccgtgatg ctcttctcga tgatcttctt ggccatcgcc
     6481 gggttttctt caaagtacgt cttgatttcc tcgtacacga cattgcccac cacgccttca
     6541 atctccgtat tcaccagctt gtccttcgtc tgggatgaga aacgcgggtt gggcatcttg
     6601 acggaaataa ccgccaccag gccttcacga acatcttcac cgctcaggga gggatctttt
     6661 tccttcaaca ggccgttggc ctttgcgtaa ccgttgatga cgcgcgtcag ggcgccgcgg
     6721 aacccggaaa ggtgcgttcc cccgtccccg ttgtaaatgg agttggcaaa gcaaaggatg
     6781 ttcgtttcat acgtgttgtt gtactggaac acaacgtcca ccagaacatc gtcctccatc
     6841 ttcttgccgt cgtactccac ttccaccttc cggacgccgg aaatggaaat ggggtcctcg
     6901 ttgatcactt ccttgttcgt gcccagctga cgtacgaact ccacaatgcc gtccttatag
     6961 aagaacgttt cccggcgcgc cggagcttca cgctcgtcaa tcagttcaat gaccaggccg
     7021 ggattcagga aagccagttc ccgcaggcgc gtcgtcaggc ggtcaaactt gaactccgtg
     7081 gtatccgtga aaatggtggc atccgggaaa aacgtgatcg tggttcccgt gacttcacag
     7141 gcacaggaac ctacagtgcg gaggggttcc accgtcttgc ctcgctcaaa tttgatctga
     7201 tagcgcttgc cgtcccggcg cacttccgcc ttgaaccagt cggacagagc attcacgcat
     7261 ttggcgccca caccgtgcag gccgccggag tacttgtacg caccctgacc gaatttgccg
     7321 cccgcgtgca ggttggtcag aaccagctcc acggcgggaa tgccgaactt cgggtggata
     7381 tccaccggaa tgccgcgccc gttatctacg atggaaatgg aaccgtccac atgaatgaca
     7441 atgtcaatct tgctgcaata gccggccaga tgctcgtcaa tagagttgtc gagcacttca
     7501 aatacgcaat ggtgcaagcc tcgttcgtca gggtccccga tgtacatgcc gggccgcttc
     7561 cgcacggcct ccaggccttc cagtttgtca atctgcgcgg cgccgtattc atgctgcgcc
     7621 gtcgtggaaa cggtggtttc aacaacctcg ttggtatctt cggacataac cgaaatatac
     7681 tacgcttatc ttaatttttt gcaagactaa tattataaaa cgcgtgcgcg cgcgtagggc
     7741 ctagccttgc aaaattccgc tgaaatcgca taaaaaaggc cctattgctc gcgttccgtt
     7801 cttttccggc tccggacagc ggagagggct tccggaaaca tttggcgcgt ttttacagat
     7861 ttgaggagag gatgaggcgt ttcacgggag gggacgaggg gaaaaagggc catggcttcc
     7921 gtggggacgg cgtatttttt ccttgccgct gttttatttt tgacccatcc gggccgcccc
     7981 caggggtttt cctcaggcaa cagagccgct ccggagttgt tccggaacgg ctctggaggg
     8041 tcaggaggaa ggcctttcgg gggtggggcc ccctccttgt gttttccttc ttttagaagt
     8101 cgtagcggta gccgatgctt ccgttca
//
LOCUS       DAWEMX010000116         8044 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3685_length_8044_cov_10.787708, whole genome shotgun sequence.
ACCESSION   DAWEMX010000116 DAWEMX010000000
VERSION     DAWEMX010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3685_length_8044_cov_10.787708"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    215..224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1348..1357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2067 a         2428 c         1972 g         1557 t
ORIGIN      
        1 aaccgggcct tgtcctgttc ggacaaaatt cccgtgcccg tgctgtcata gcggtccaaa
       61 accatttggc ggatgagcaa agtcacctga aggcattcca gtccgggcct gggggcagtt
      121 tccattttcc cgcggtcgcc ggaagccacg gattgccctg ccgcaaacgg gaacagaccg
      181 aacgccagcg ctgcggaaca tgacaagata aacannnnnn nnnnactttt actaaccccg
      241 ccggaaaaag acggtttcaa aaataaacat ccgcccctgc cggcgggcga cacgggcgga
      301 tgcaggaaac gtacggaaag cggaaaaact tacttctcca cttgaacgat cgcgttcttt
      361 tccaattcaa tcaccacgcc ttccgcaatc ttgagagaaa cggtgcgctc attcaccttc
      421 tccacaaaac cgtgcagccc tgcgtttgta atcaccttgt ccccgcgctg cagggctgcg
      481 atgcgcgcct gctgctcctt ctgcgccttc ctctggggac ggatcagcat cacccagaac
      541 aggacgatga taatcacaaa catgaacatg gggctggcga gaatctgctg aaacatattt
      601 gcgggttcct gcccggcggc gcccgccgca tcctgagcct gtgctaacat aaagatattc
      661 ataaaatgtc gtttgcttta taccgcgcga taaacgaatc cttgaaggaa ccgaacgtgc
      721 cggcggctat ggcctcgcgc gcctgggcca tcagccggag atagaattcc agattctgaa
      781 aagaaagcaa tcttaaagcg agtatttcac cgactttaaa caaatgccgc acgtaagcgc
      841 gggaaaattg cgtgacatgc ggatgtccct ccgggtcaat tgggcgggaa tccgtcgccc
      901 agcgctgatt cttgatatgc atggggccgt ccggagtcag cgccacgccg tggcgagcca
      961 ggcgggtagg cattacgcag tcaaacatat ccacgccgcg ggcaatcatt tccagcaatt
     1021 gggggggggt gcccagcccc atggcgtacc ggggcttctc ctccggcagc cagggagcgg
     1081 aatgatcaat ggcgcggagc atttcatcct ccggctctcc cacggaaacg ccgccgatgg
     1141 cgtacccgtc aaaatccatg gcggccagtt cttccgcgca cttctttctt aaatccgcat
     1201 acacggaacc ctgcacaata ccgaaatggt gctgacgccc ttccccggaa cgggggcgat
     1261 gttcctgcac ccacgccttg cagcggcggg cccaacgaag cgtgtacccc agggaggctt
     1321 ccgcgtactt cctgtcgcag ggataagnnn nnnnnnncgt caaacagcat ggcgatatca
     1381 ctgcccaagt ccgcctggat ctccatggac cgctccgggc tcagcatcat gtacgccccg
     1441 tccaaatggt tctggaaacg caccccttct tccgtaattt tacggagctt ggcaagagac
     1501 cagacctgga acccgccgga atccgtcaaa atagggcggt tccaggagga aaatttgtgt
     1561 aatccgccca tttgcctaat caaagcggaa ccggggcgca gggacaaatg ataggtattt
     1621 cccagaataa tctcagctcc cagcatctcc aaatcctgcg gatgcatggt tttcacggaa
     1681 ccctgcgttc ccaccggcat gaaaatgggg gtgggaaccc gtccatgcgg caactcaagc
     1741 gaacctaggc gcgcagccgt ggaggaatct ttctgatgaa gtataaaagg catcggaatc
     1801 agactttctg caatttgata aagcgggcgc ggtaaatcgg ctttcccatg gcctcccatt
     1861 gaagctggaa atccgtacgg ggataaaaaa aatcgtcctc atcccaactg gcacggctaa
     1921 acagaccgct gcgcgccaca tggtccagca cccacagaaa atatccttca tgatccgtct
     1981 tgaacaggaa ctccccgcca tcggacaggg aacggtgaag cacgggaaga aaatcctcct
     2041 gcaccagccg gttcttatga tgcctctcct tgggccaagg atccggacat aaataatgca
     2101 gccgggaaat gcagccgggc tgcatcatcc attccagaaa gtaacggctt tccacacgaa
     2161 acaccttcac gttatccaac ccgcggacgg ccgcccggga acaaaccccc cgtacgcggc
     2221 ccaaaagccg ttctattccc agaaaacggc gctccggata atgggccgcc atctgaagca
     2281 gaaatcctcc gtccccacac cccaaatcaa cctcaaacgg cccatccgcg ccaaaaatat
     2341 cagaaggcgc cagcgccttg aaaaaatcgt ctggaataaa ggtcggattt atcatgggaa
     2401 tcaacgtgcg gataagatgc gttacaaaac ggactctgtc aaacgagaag aacccctccc
     2461 cgcaaacatg gcgggaagag gcccggaaaa acggaaaaaa taagccagaa cggcccggaa
     2521 ccgtcagctc cggcaataaa ggcgattata aattttacgg tcaaaacggt aatagcgcgc
     2581 ggcgcggtgg ggaaccccct cctccttctc atccagcggc acaatgtagg ggcgggaagc
     2641 gatttttttg tggaaattac gcacatccat gggcttgccg tgaatctcct cattcagccg
     2701 ccggagctga agagccgtaa acttggcagg cagcatgtca taaatcagcg caggctcctt
     2761 cttcacggaa gaacgcactc tctccagcgc cagatcaata atatcccggt gatcaaacgc
     2821 cagttccggc aaatcatcta caggaaccca gcgggtcttg tggctttcca tcagtttgcg
     2881 cagcttggtg gaaatcctga gcatggccat gtaggccacc gtcaccaggc gtcctatctt
     2941 ctggccggaa acagcctcca cccatgccct gtcctccgga ttcttgatgc gcgatggcgc
     3001 gccgaaggta tggaactgtt ccaaatgggg ggaagacatg ccggtcatgt caaacaaaac
     3061 gcggcgggca gcggcatcca gttcttcctc ctcataaatc aaatcgccgg gcaatttgga
     3121 aatggcgccg gtatggccgg gttcaaattg cgtcttctca accagaagga ctttcaaacc
     3181 ttcttcatcg aacccgatca acacacaatc cactgagaga tgcggataaa atgcctgcct
     3241 gctcattatg ttatctatta ttgtagtaag ttcccagaat cgcatcaacg ctacgcaagc
     3301 gaaccagcca agtcaaccaa aatcaaaata gaaacggcaa tgaaaagccg gcccgcttgg
     3361 ccccgggaaa catcacgcca cttccggaag actgcttctc acgggcggaa aaacgcgccc
     3421 gcgtcttgca tttcatcatc tccccgtcca tataataaaa tcatgcagac aggtatcctc
     3481 aggggaatgt ccgtgctggc cgggctgcta tggctggcat cctgctcgtc ctctcccaaa
     3541 agccgggatt atccgggcta catgacccgc ccctatacca tccgggggca ccgctaccat
     3601 cccatgaacg tggaacaggc gctgacgtat gaacaaacgg gaattgcctc ccattacaat
     3661 gaatgtgccc tttgggggct cgtcagcgga aaaacggcaa tcggagaaaa cgtgcgcccc
     3721 tggcacctgc acgcagcgca tcccaccctt cccctcccgt gcgaagtgct cgttcaatcc
     3781 cttcgaacag gcaaaaccgt aaaagtccgc gtaaacgacc ggggcccctt catcaaaaac
     3841 agaatcatag atcttagtga agaagccgca gaacggctgg acatgaagca ccatgggctg
     3901 gacaaagtca gaatcaccgt cctttccgta ggagacggga aatggaaaaa agaagcgccc
     3961 ccttgcgcca ctccggcctg attccctact tcaacacatt ccgtgtggtc ttaaaatgca
     4021 tcttggccac cttgggcagc atttcttccc catgcagggc caccagctgg cgcgcagctt
     4081 tcaccacttg gccggaagct cccagcggca acttcatgcc ggcgccctct cccgccgcat
     4141 ccatccagtt cacaaaacgt tcgcgcgcca gaatagatgc cgcagccaca gcaacatcac
     4201 tctccgcccg gacacgctgt tccagccgga cacacacctt tttggcggca agcgccctct
     4261 tcagaacaaa cggattggcg aactggtcgc tcaatgccat gggacaatcc ggcaccttgg
     4321 ccgccagccc ctcaatcacc gtagcatgcc cccaggcgag aaaacggttc aaattcccga
     4381 actccggata aagctggtta taccgttccg ggccaatgct taccagatga aaccggatgc
     4441 ccggcaccct gcggatcccc tccgccaggc aacggattct ggatgaagac cctaccagct
     4501 tgctgtcaca aacgcccagc ttgcgcaatg cggcgccgat gcccccgtcc acataaacgc
     4561 cggccaccac cagcgggccg aagtaatctc cctttccgct ctcgtcaatc ccgaagtggg
     4621 gaacaaaacc gcatccttct tccatctctt ccggctgggc caaaactccg gtcacctgcg
     4681 gttccagcgt aaactctata aaatcatccg ttccctttcc ctgcagaaga agcttgttct
     4741 tcttctcgta aaccgtcgcc agcagggaag gcccggcaaa tgaaaaacgc gcatagggaa
     4801 cctctcttct ttcaaacccc ttttcctcaa gaatggactc aagccttccg gcctgggcgt
     4861 cagtcagcat ggttgtaaac gtggttcgca tatatccttc ccggagccgg aacactccgg
     4921 ggcaagcaat ggaagcatcc caaccattcc ggtcaagcaa aattcccgca tcacgcggtt
     4981 gaggacgtaa aaatcaagca tgccacggat tatgtgattt tttaggagac aaatcatgca
     5041 cgctgaacta taaaactcat acattcatat cattctgcac tccatatggc taaaacatta
     5101 acaaaacgag acctcgttaa caaaatcagc gccgaaacca acttcactca gattgaagtg
     5161 ttcgacatcg tacagcgtgc cgtggacatt atctccagca cccttgctga aggcgaccgt
     5221 gtggtgattc gcaatttcgg aaccttccag gtgaaggaag tgaagcccaa agtaggccgc
     5281 aaccccaaaa atcccgacca ggatgtgccg attccggccc gctccgttgt caaattcaag
     5341 gttggcaagg aattgaagga tcaggtcgcc aagttgaccg cctccaagta atccccacag
     5401 ggatcagaca gattactccg ggcgtctcca tcaagcaatg gaggcgcctt tttgtttgca
     5461 atttgcgcgg cattcgggaa tataagggaa cgtgagcatg ttccggatac agcaccttct
     5521 tctgatcacc gtagcagcac tttgctcctg tgctacagaa cgcacggtag ctacaaactt
     5581 tcagccccgg caacgccctg attcggaaaa aacggacgca gagctgatgg atgccctgga
     5641 agcgaaattc gccggcggct tcggccagca gacggacaag gaaggaaaca ccaggatggt
     5701 gtcccaaaaa cggagttcct ttgaagggct ccggtacagc ggcaacacgt ctcaaatcga
     5761 aaaaaaggct tttgaaaccg catcctttga aaaaaagaaa tttgacggtg cctccacccg
     5821 gtttgaaacg aagaaatggg acggcatcaa atccttcacg gatgataaac tggaaacacc
     5881 ggacttcatc gcccgcgcca ggggagtaaa tacccactcc tggcaggatg cctccaaaca
     5941 atacgccacg cagcaatccg actttcatgg acgctcctgg aaagatgcgg acaagcgcct
     6001 cggccacact gtcaaccggg acattgaagc caagagacaa agctttggac agccaactat
     6061 catgagtgcg cgacaggccc aggcgcatac tattcaggaa acgcgggaaa tgatgggacg
     6121 cacggactga tgcgccgatg tttcggacgt gatcccatgc cacatttcag attcgacatt
     6181 gttttcagcc tccatctata attctgcccg tgagtgccaa agacgttcct ccttccatca
     6241 ccctgcctac cagcgactac tataccattg tcaaaatgag caatcatgcc gtcgtgggag
     6301 tattccgcca gcatgtcttt gcgcgccgca gcacgcgccg ttatgcgccc cccatccctg
     6361 aagaacacga ctcctattgc gttaaaagaa ctccggagcg tgtcatggtc caaatctttc
     6421 atgacggcaa cgaagtttac cgttgcattt ttgtgccgcc tgccgattat tgacggcagg
     6481 acaaccatga aagctccttt tttcagtccg gccttctgcc tcattacaag cctgattaca
     6541 gcctgctctt ctccctccgg ccccaatgcg tggacgccgg tcacttccgg tcctccgcgt
     6601 gcgcccatcc cccagaacga agttctggtc attgacgaat accccatggg ggaatacacc
     6661 aacgtggggc attttaacgg gccggaaggg cgcattgtcc atgtctctat ggaagacaag
     6721 gaactaatcg actacttcac caaggaagct gcggccatgg ggggcaatac cgtcgtcatc
     6781 agggaaccgc gcatccgcta ccgttccggc gaaggcaaga gcagccgggt ggacgtcatc
     6841 tatgttcggg aagaaatggg aacacacggt gcggaagacc tgggcatgga ctcgctcccc
     6901 attgatgaag aatctctgcg gatttattag agccgccgga aacgtcccag gtgccatttc
     6961 ctgcccggag ttttttcagc tctttttatt ggccgcaccg caatgtgggc actccgcacg
     7021 ggaattcccc ttctccatat aaaaccggaa tccgcatatc cggcactgga acttgcgcct
     7081 ccgatccagg aaagatcgta tccatcccac agccaggcaa acagacacca cagcgcaaaa
     7141 accgccggcg cccagtaaaa acaggcgcat caaaaaatct tggaggatca tattcacggc
     7201 tcttcctcct tccattcgat ggacatggtg ccgctccgcg gctcccggga cggaacccag
     7261 cgcaaagacc gggcgaacct cattgcggac tcatccgctc cattacttcc ggaagactcc
     7321 agtaacaaaa cttgagaagg aacgccccat acatccacca ccacaaaaaa atcactcctc
     7381 agccccgtgt gatcttcact tctggaaaaa ccggacaggg agccggctgt aacaatccgt
     7441 ccatccagcc ccggggaaca aaaagacaaa tagggaatgc atgttccgga aatctccaag
     7501 gaaggattca tcatggcgcg tatatgaaaa tccggatgct gccacatacg ccagacctga
     7561 tccgccacag tgggcacgga cacggggggg agaggggcca gaggcgtatc tgcggaacac
     7621 ggagaaggca aagtcacacc catttccccg gtcgggccgg ccgtcaccgg atcagcccaa
     7681 acggggcctc tcacaggcag aggagtattg cgcagcacat actgatgcag gcgcggatta
     7741 tctgcaggca gcagcaccac ggcacccggc tgatctcccc tgctgcggat ttccggcacc
     7801 tccacatgga caaaaaggcc ggcggcaaaa gcgcaaacgc caaaaaaaag catccacaga
     7861 aaaatacgcc agcgcaacag gccggtttcc ctccagtcaa acgtcaatcc caaatgaagg
     7921 tcgaaaattt tatcccggct ctttttattc agcttcatga ggtaacaagg gaaaaaatca
     7981 atccgcgggc tcggcggcaa tataacacgt catccccttg gcaagcacca tatccaccag
     8041 ctgc
//
LOCUS       DAWEMX010000117         7994 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3716_length_7994_cov_12.056178, whole genome shotgun sequence.
ACCESSION   DAWEMX010000117 DAWEMX010000000
VERSION     DAWEMX010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3716_length_7994_cov_12.056178"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1660 a         2100 c         2530 g         1704 t
ORIGIN      
        1 gctgatgaag atgacgacgg ttgccgtagc aaggccttct acggcgccga tggccagatg
       61 aatgggcagc atcaattcaa caaattgggc gaagggcagg gctgagatgc cggaggccgt
      121 tgtttccaga acgacggaga aagcccccat ctggagcccg atgacggcgg cgatgacgct
      181 ggccagcgtg atacggcccg tgccggtttt tgttccggcc aggggcctgt aaatgaaagg
      241 gtaagctacc aggcaggaga aaaaccccat attgaaaata ttgcagccca gggccagcag
      301 tcccccatcc gcaaagaaaa gggcctgcac cgtcaggatg gccgccatga ccaggaaccc
      361 cgcatacggt cccagaagga ccgccaggat gaggccgccg cccagatggc cgctggagcc
      421 ggtgcccgga atggtgaaat tcagcatctg gcaggcaaag atgaaggctc ccagcacgcc
      481 catgagcggg acgagccgca tttctttttc ctgcctgacc ttacgtgcgc agagcgttgt
      541 aattgcagcg gaagccgccc acatggtgca gcctaccgcc ggtgagatga agccgtccgc
      601 catatgcatg atgttgatgc ctttctattg aatagtgtga agtattgctg aaagccccgt
      661 accgggcaga ttggcgccac tttagcataa acgccgcaaa gccgaagaag gaaatgcggc
      721 aacggaggaa agtatgttcc ttccgcttct cggttttggt tcccgtgcgg agccggtaag
      781 gcctgtttcc ggcaaaagag cctttccgga gggggaacgg gagggagggc gcgtttcttc
      841 gggtgcatcc ggcggcaatt catctgccgt cccgtgccgg caaagggtgt ggacgggttg
      901 ggcgtgatgc aggcgggaaa actgtctgct agacgggacc catgatgaat ttttccatag
      961 ccaggtgttt gttttccata ggaggatgca cgacaatcct gggcaatatt tccgcagaca
     1021 cggccgtgtc ttccgccgga gccatcggag gcctgggcgt tctgacgatt gccgtgaatt
     1081 acacgctgaa tctgctcaaa aagaaggatg agcagttgat ggcgaaggat gcccgcattg
     1141 aagccctgat ggaccgcctg attgacaaat gcccgaattg cgagctggcg aaagcagcca
     1201 acaagtccct gctggatgat gaatgacggc aggcgcgggg gactatctcc cgtcagcgtc
     1261 cgtcatgcgg gtgtaaacct cccgggttcc gtccgtgttc aggatcagcg tgtttctgct
     1321 gagactgtca atggtggagg tagcggtgaa gagggaggaa ttgccgtttc ccatgctttt
     1381 ccccgtcagg atcaggcggc tgccgtccag ctgccagcct tggtagagaa gcgtgtgcat
     1441 gttgatggaa acggccctgc cgtccggcag aagctgcatt ccttgcacat gcccaggctg
     1501 cccggggacg ggctgggtcc aggagccgga gaggctggcg ggagtgggct gcatggagca
     1561 ggatgccgcc agcagaaaca gggccgggag aaagagggaa aatttcacgg cgggggagtc
     1621 agcgttgttc ccggatgatt tccgtgccga tgccgtccgg agtgaaaatt tccagcagca
     1681 gggtgtgggg aaggcgtccg tcaatgaagt gcactttgcg cacacccgcg ttcagggcgt
     1741 caatggcgga gtggatttta ggtatcatgc cgccggatac ggtgccgtcc ttgataaggt
     1801 cgagggcttc cgtacgcgtg acggatttga tgagggtgga gggatccgaa gggtctttca
     1861 tgattccggg gacatcggaa atatagatga gtttcaccgg cttgagttcc ttggcaaggg
     1921 cggcggctgc caggtctgcg ttcacattga gcggtttatg ggtgcccagt tcttttgcca
     1981 gcggagagac gatgggggtg atctggagtt ccagcgcttc cgtgatgcgc tccgtcaggc
     2041 aaccgatgac gttgcccact tcaccgatat ctatggggtt gccctgttca tctttttcat
     2101 ggatgcgttc cccaacgaag atttccgtgc cgggaatgcc tacgcccttg ccgccgtagt
     2161 cgcggaacat ttgaaccaga ccgggattga tggttccgga aagggtgcgt tccacgatgg
     2221 aaatggcttc cggagtcgtg acgcgaagcc cgttgacgaa acgggcttcc agccctgcct
     2281 ccgccatcgc cttggaaatg gctttgcctc cgccgtggac aatcacgggg ttgaagccca
     2341 ggacttccag cagaacgatg tcccgcatga gcttcttgac caggcgggcg tcatccatgg
     2401 cactgccgcc gaatttgatg aggaatgttt tgtcccggta ggcctggagg taggggaggg
     2461 cgccgacaat gacggaggcc tgttcaatca ggcgggtgat tttggagtcc atgtcagtaa
     2521 tgaaacggta agagttttaa cggaaggaga gggatatttt gaggcaggac ccgttcaaag
     2581 caggcgcctg cttctgaagt aaaccagggg caggatgatg gaaacgagca tgaccaccag
     2641 ggagatgtag tagccgtatt cgttttccag gccgggcatg tgggcgaagt tcatcccgta
     2701 aatgctggca attaacgtgg ggggcatgaa gaccacggcc gccacggtga agattttaac
     2761 gatttcgttc tgttcgatgt tgatgaggcc cagaacggcg tccagcatga agttgacgtt
     2821 gctggaaagg aagttggcgt attcggaaag ggaagtgacg tcccggctca gaggacgcag
     2881 cttgatgtac agcgtggagt tggtgtcgtc caaggcctct tcgttgctgg cgtacgtgag
     2941 caggcgcaga aggttgacca ggcagtcccg ctggcgggtg agcaggtcgc ccacgcggcc
     3001 caggtcttgg agcgtcaggc gcagggcgct ggagacgggc acccgcttgc tggccttgcc
     3061 ttcaggttcg tccctgcgga agatgttttt ggaaaggcgg tccagttccg cgccgaaacg
     3121 ttccagcacg tccgcctggc ggtccacgat ggtttccagc aggccggtga aaacttggtc
     3181 ccggctgatt tgcttctggc gcaggagctg gtgggaaaaa acgcggaagg aataggagtc
     3241 cgaatggcgg atggtgatga ttttcgcccc catgaggacg aaggtgattt ccgacaggga
     3301 gggggattcc gtatccacgc ggctgagaac cgtggtggtc atgaagcgcg ctccgtcctc
     3361 cgtgtacaga cggctggtgg cctcaatctc ccgcatttcg tcataggtcg gcatttccag
     3421 cccgcagagg cgttccacaa acttgatttc cgccggttcc ggggtaagga ggtcaatcca
     3481 gaagacggaa tccaagtgtt gttccgccgc atccacctgg gtggtgcgct caatgccttc
     3541 cggggtttga cgatagagcc ggatcatgcc gttgatttcg gtggggggat ggaacgatgc
     3601 cgtgcatgag gttgagtgac acccaacgca ggcatgatgc cttgtttgtt cagggaaggg
     3661 aagtaaaaag ggtgacaaaa agaggcccgg ttcccaaggt gggaaccggg cctgccggaa
     3721 aagggatgcg cgcctcttag gcgttttcct tgacgaccac cttgagcgtg gcttccacct
     3781 ggggatgcac cttgatgacg acttcgtgtt cgccttcgct cttgatcggc ttttcgagtt
     3841 caatcgcccg gcggtccact tccacgccct gagcggtcag ggcatcgtga atgttctggt
     3901 tggtgatggc gccgaacgcc ttgccgccct ggcccacttc aagggtgagg tctacggtca
     3961 gtccgctgat cttggcggcc acttcctgag cggcgctgag ttcttccgct tcgcgcttgg
     4021 cgcgggcggc ctgaagattg gcgatgaaac ggcggttgga ggctgttgct tcgtgggcca
     4081 gacccttggg gatgaggtag ttgcggccat agccagcctt aacggtcacc aggtcggctt
     4141 ctgcgccgag cccttcaatt tttgttgcga gaattacttc catcgttgcc atggctaaaa
     4201 agaaatggtt atttgtgagc ggttgaagta gtaagtattc gctcgaatgt caagacaaga
     4261 gcgataaaaa gggcgttttt ttatgttttg ggaggaaaaa tcctcccggt tgtcatgatt
     4321 ggtgacattg cgcttgcttc acgcggcagt agtatgtagg gaaaggaacc gcatttatcg
     4381 cgttctctcc cggaggggtg gggttcgccc gcgggtacat tacagatatt tgatcttatg
     4441 gctattcagc gcgcattgat atccgtttcc gacaagaccg gcctggagga gtttgccaag
     4501 ggcctgcacg agtttggagt agagcttatt tccactggcg ggaccgccgc tttcctgaaa
     4561 ggcctgggcc ttccggtgat tgagatttcc gactataccg gagaaccgga gttgtttgaa
     4621 gggcgcctga agacgcttca tcccatggtg cacgggggcc tgctgcaccg ccgcgacaat
     4681 gaggagcatg tgcgccaggc taaggaaaac ggcatcaagc ccatcgacct ggtctgcgtg
     4741 aatctgtatc cttttgaaga aaccgtcgcc aggcccggag tcacactgga ggaagctatt
     4801 gaaaaaatag atatcggcgg gccgtccatg ctccgttccg ccgccaagaa ttacgcttcc
     4861 gtcacggtgg tttcggatcc cgcggattac gcgcgcattc tggatgaaat gcagacccac
     4921 aagggggata cgaccctgaa gacccgtgaa aacctggcgg tgaaggtgtt tatgcgcacc
     4981 tccaattacg ataacgccat cactaattat cttggccacc agagcgcgga aagcaccaag
     5041 ggcagcttct gcatctgcgc tcctctgtat caggaattgc gttacgggga caatccccac
     5101 caggaagcca gcctgtacgg cagcttcggg gatattttcc accagcttca gggcaaggag
     5161 ctttcctata cgaacgtgct ggacattgaa ggggcagccg agctgattac ccagttccgc
     5221 cgcccgacgg tgggcatttt gaagcacacg aatccctgcg gcgtgggcca ggacgacgaa
     5281 gacctgcgca acgcatggca gaaggctttt gaaacggata cgcaggcccc cttcggcggc
     5341 gtgatcgtgg tcaaccgccc gatgacggaa ggcctggccc gtgtgttgag cgccattttc
     5401 acggatgtca tcatcgctcc ggagtatgat gcggaagccc gcgccattct ccagaagaag
     5461 aaaaattgcc gcatcatccg catgaacacg gaagcctgga tgaaggcgcg ccgcgaaccc
     5521 atcatccgtt ccgcgcccgg cgggttcatg accatgaagc gggatacgga cgtgatgggg
     5581 ctggacaatc tggaagccaa ggtggtgacc aagcgccccc cgaccgagga agaattgacc
     5641 gccatgcgtt tcaactggcg cgtcgtgaag caggttcatt ccaacgccat cgtttttggc
     5701 ggtacggacc gcacgcttgg catcggcgcc gggcagatga gccgtgtgga ttccgcccgc
     5761 attgccgtct ggaaggccgg gcaggccggc ctggatctga agggcagcgt tgtggcgtcc
     5821 gacgccatgt tcccgtttgc agacggcctc caggtggcga ttgacgccgg agccaccgcc
     5881 tgcatccagc ccggaggttc catccgcgac gaggaagtga ttgccgccgc tgacgccgcg
     5941 ggaattgcca tggtatttac gggacaccgc catttcctcc attaatttga atgttcgtct
     6001 ccgccccttt gtcttaaaac cgatatgctg ctaggtgtaa atatagacca catcgccacg
     6061 ttgaggcagg cccgctacgc gaccatgctg gattccttca atgtggagcc gagtgttttg
     6121 gacgccgctt atgcggcgca aaggggcggg gcggattcaa tcaccctcca tgtccggggc
     6181 gaccgccgcc acatgcagga tgcggatgcc ctgagtgtcc gggagagcgt ggctcttccc
     6241 ctgaacctgg aaatgggaaa taccccggag atggtggatt ttgccctgcg gctgaaaccg
     6301 gactatatct gcatggttcc ggagaagcgc gaggaaatca ctacggaagg cggcctggac
     6361 gccgtttttc atgaaaagga tctggctcca accatggcaa gaatggccga caacggcatt
     6421 caggtgagcc tgttcattga tccggaagtg ccccaggtgg aggctgccgc ccgcctgggc
     6481 gcccccatga ttgagttgca taccggatgt tttgccaacc attccggcag ggagcgcacg
     6541 gaggaactgg cccgtttgaa acgcgccgcg gaactggccc attccctggg cattcaggtg
     6601 aatgcgggcc atggcatcaa ttaccagaat ctggaacagc tgctgtctgg cgtcccttgc
     6661 ctgcatgagc tgaacatcgg ccatacgatt gtatcccgcg ctctttttgt ggggatggag
     6721 caggccgtga gggaaatgcg ccaggcgatc gaccgcctga gctgattgcg ccgtttcaat
     6781 tgatcccggt tgccggagta tgagccgcat tgcaggattg ggaatggatt tggcggatct
     6841 ggaacgcgta cgccaggccc tgcggaaaaa cggggaggcg tttgcgttgc gcatctgcac
     6901 ccctgatgag tgggcttatt gccggaagca cgcggatccc gtgccgcatc tggcggcgcg
     6961 ctttgcggct aaggaggccg tggcaaaagc cctgggaacg ggaatcgggg cgaagtgcgc
     7021 ttttacggat gtggaggtgg tgcgcggtga tgcgggagtg ccttccattc ttttgcacgg
     7081 ggctgcggga gtgaccgccc gggagctggg cattacgggc tggctcctga ccatgaccca
     7141 ttccgggttg agcgccgccg ccaccgttat tgccttggcg caataaaaaa gccgttcctt
     7201 ggggagaaag gaacggctgg cattaaaggg ggaaagggaa gttattttct ccttctgcga
     7261 gcggcaaatc ccgcgaacgc aagcagagac aatgccgctg tggagggttc cggaacagcg
     7321 gcataagaaa cggagagaga actcagtttc gtgccgttca agccgtcatt attccaaccg
     7381 ggagcacgat gtccaatgct gtacatctcg ttttgggtat ctcctccgtc cgcggttccg
     7441 gtgtaccagt cgatatcgct gatagaaacg gttgcggtgc tggcggcagt accattatca
     7501 agagtaacgg ttacgtcatg ccccaggata tcaacggtta cttttgtcca cgtatttgcc
     7561 gtcagtccgg cttctgcgat tgctcctccg gaggagaggt tgccggagtt gatggcaaca
     7621 gccccattgc tgagtcccag gctgaagccg aatccgctac gggcaacgtg aatgatattc
     7681 tcaagggaat tgttgcctcc ccatgagatg tccatggtga tgctcatggt cccctccccg
     7741 gaaagggctc cgttcagcat ttcccggaat acatttgtac cgcgttcgtc aaaggtggaa
     7801 ccggtaagaa ccgtcccctt ctctgcatct tgggcagtat tgaaattacc tgtagcagtt
     7861 ccccccatga cgacagccgc ggaggaaaaa tcccatgtat aggtagctgt ctccgccgcc
     7921 tgtgccgcac cacagaaaag cagccctgct gccagcggca aatatttttt aatggataga
     7981 ttgatagtca tatg
//
LOCUS       DAWEMX010000118         7822 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3807_length_7822_cov_11.824771, whole genome shotgun sequence.
ACCESSION   DAWEMX010000118 DAWEMX010000000
VERSION     DAWEMX010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_7822_cov_11.824771"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    383..468
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6413..6422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1703 a         2449 c         2050 g         1524 t
ORIGIN      
        1 tggcccttta cttcccactg gggccttatt ccatgatttt tatccctttc gcaggcacgt
       61 tttttccgtc aagacatgac tcaagcccaa accatcacca gcaaggccaa gtccaacctg
      121 gcctttgccc tcattgatct tcctgaagaa gaacgccgcc atatggcgga attttacgct
      181 ttctgccgca cggtggacga tatcgtggac gaaccgggca tgactccccg cgaacgccac
      241 gacgcattga accgctgggt ggaggtcatc aacggcggag aaggcctgga actgacggaa
      301 ctggaggagg acatcatagc cctcatccag gatttggagc tggacaccac tcccatgctg
      361 cacctgattg aaggctgccg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cagccgcgat
      481 gaactgctgg actacaccta ctgcgtagcc tgctgcgtgg gcctcacctc cgcccgcatc
      541 atgggggcgg gagaagcctc ctatccgtac gccattgccc tggggcatgc ccttcaaatg
      601 gtgaacatca tccgggacgt ggcggaagac tgcggcaagt ccaaccgcat ttatttgccc
      661 aaggaggata tggaccgctt tggatacacc ctggaagacc tgcgctgcag ggtgtattcc
      721 ccccggcttc aggagctgct gaaatatgaa gcggatctgg cggaaacttt ctttgcggaa
      781 gcggagaggg aatacaacct tctcagcccg gaagacaaag ccgcccttat tccggcgcag
      841 gccatggcgc tcatttacca taccattctg gacaaaatga aggcgagcgg cttccgtatt
      901 ttcgacatcc gctaccgcgt caacaccttc cacaagctct ggctcctgtt ccgcaccaaa
      961 ctggatattg accctcaatc ctactacgac aaatccaaag cgtattatga caagctggta
     1021 ggctttcttt cccgccccgg cagaaaggat gaaaaacagg ggcctcccag gtgaaccatg
     1081 gaaaagctgc ggatttttct cccggccctg caagaattat ccttgtcccg aaaaccgtgt
     1141 tcctgtagga gttgcagcat gcaaaacccc gtggcctccc tgctttttat tctcgcaatg
     1201 ttgacagggc catgcccggc agcggattat ccggaacgga cggagcgcac gcaatccgca
     1261 ggaaaccatg tctggcatat agacccggac aagggaaatg acggcaatcc gggaacggcc
     1321 ccatccacgg cgtggaaaag catggcgccg gccaaccgtc tcatcatggc gcggggagat
     1381 acgctcgtca tccatccggg ggaacatgcc gtctccctgg cgctgatggg ggaaggttcc
     1441 aaacaggccc cggtcaccat ccggttcatg cccggcaggc atatcttcaa acacggtgcc
     1501 ctgatgaccg gaaaaccgca gatttccaac accaacgatg cgcccaacga gcccaaggcc
     1561 atggctatcc gcctgatgga agccaaaaac atacgcctgg agggaaagcc gggagccacc
     1621 gacatcctgc tggaaggaaa agccatcttt gtctgcatgg aacatgcgga aaacgtttcc
     1681 ctgaacggcc tggggtttga ttacctgcat ccgaccatgg gggaatttct ggtcacggaa
     1741 gttgaagggg ataccatgaa agcaactatt ccggacggaa tcctgtacac agtgaaggat
     1801 ggaaacctga cctggcacgg acccggctgg gaattccgca tgggagggta ttccaaagtt
     1861 ttcgattccg cctccggcac tttccagggc cgtttcgacc ccgggaaaac agtcatcaga
     1921 gaattgtctc ccggaaaaat cagcatcacg ttcaaggaag gctcaccaac catgaaaccg
     1981 ggccaatcct accagaaccg caatacccgg agggactgct gcggcttctt tcaatacaga
     2041 agcaaaaaca tcctctggaa taactgccat atttactata tgcacggcat gggcgtcgtt
     2101 tcccagttct gcgagaacat catgttcagc cacttgaaaa tagccccgcg cccccgttcc
     2161 ctccgcacca attcctcctg ggcggataat ctgcactttt ccggatgccg gggaaaaatc
     2221 atcgtcaagg attgcgtgct gggagcctcc catgacgacg ccgttaatgt tcatggaacc
     2281 cacttgcgca ttatagacag gccggccccc aacaaaatca ccgtccggtt catgcatccc
     2341 cagaccttcg gctttgacgc ctttgccgca ggagaccgga tcgattatgt ttcctgcaac
     2401 acgctggtgc cttatgcatc caataccgtt tccggcgtca aacaactcaa tgaaaaggaa
     2461 atagaactta cattgcaaca tcccaatccc gggaacatcc agcctgacga tgttgtggaa
     2521 aacgtcacat ggacgccgtc cgtccacatc agcaatacgg tatgccgcca cattcccacc
     2581 aggggcttcc tgctcaccac caggaagccg gtgctggttg aacggtgccg gtttgaaaaa
     2641 acgggcatgc ccgccatcct ggtggaagat gacgcctccg gctggtatga atccggcgtg
     2701 gtcaggaaca tgaccatttc ccgcaatacc tttatccaat gcggagaagc cgtcatccag
     2761 atcgtgccgc acgctccacg gccggaaggg gacgttcacc ggaacatcac cattaccgga
     2821 aacacgtttg acctcaaaaa cggaaccgcc atccgcattc gccataccgg tgacgtcaag
     2881 gcggagaaaa acactttcac caaagacggg aagaaaatcc ctgaggaaaa ggcggtggac
     2941 atccggtaga aaacctcttt cttaccggtg ccgggaaaca tgcaccgtta cggaaacgct
     3001 gtccgtccgc ggcagaataa acttgcgcag ggtaacaacc acgtgccgcg cgccgaattc
     3061 cttcaggcaa acctttccca tatcctccgc cagcgtttcc accagcttgc gtggacgcgc
     3121 catggcctcc gcccggagcg cacgggcaat ggaatcataa cagacggttc tggaaaaatc
     3181 atcgttcagg ccggacaaag cctcctccgg ataaaaagag atgtccgctt tcagggtctg
     3241 catggaagag cgctcctcgt ccggaacgcc aatgaaggta tccagttcaa gaccattgat
     3301 attgatggaa tcctgcgtat cggaagggag cgcgtaacgg cgcatcagcg tgcgcagaac
     3361 aagcaggtcc gggacgatca tgtccggcct ggccttggaa agctgcgctg cgtcattgta
     3421 gccggtcaaa acggcaatgg atgtgatgcc cgcgtggtgg gccgtttcca catcatgctg
     3481 catgtccccg ataaacgcgg tttcatgggc atgcagcccg tgctgggcca gcagggtatg
     3541 aatgtgggca tccttatggc ggatgcccgc atgaatggct tcaaaatatt ccatcatgcc
     3601 cagctccctg cattgggtat caaactcctt ggcgtccacg ctggtaagaa taaagcagcg
     3661 cacgccgcgc gcgcggcaga actccaggaa ttcccgggca ttcggcagca cttccaccgg
     3721 agcattggaa acgcggaagg cgtaccggaa atggtcttcc agttcatcca gatccgcgtg
     3781 gggcagcacc cgggcataat agtccggata gggaagctgg aattccgcgc gaaattcatc
     3841 cctgttcatg cagggtttcc cgtattggga aaaaacgtag ttggaggcgt ccagcgtcag
     3901 ggccaggtcg tccaccaggg tgccggacca gtcgaaaatg atatttttga acatgggaat
     3961 gcgtatgaaa aaaactcagt ccagggcgta ccgcatggcg gccaggccga aaaaggcggc
     4021 cgtctccacc cggagaacga tagggccaag cgtgacggga acaaaccccg cttccaacgc
     4081 catggccgtc tcccggtccg taaaatcccc ctccggcccc accagcagag aagcgtgacg
     4141 gacggaacgg gaacgggcgg cctccagcac ttccctgacc ggccgcactc cgggcgccag
     4201 agaggcgata atattcagtc cttccggaat tcctccgcgc agccattccg caaagggaac
     4261 gggaagcgcc acttccggca gcgtgttctg gccgcactgc ttgcaggcct ccaaggcgat
     4321 gcgctgccat ttctgccttt tggcctcagc ctcccgggca ttcaggcgca cgatcgtgcg
     4381 gtccgtcacg agcggaacaa tagcagaaac acccagttcc acagactttt ggataatcag
     4441 atccatgttg gcccccttgg gaacagcctg gcacaacgtc aaatgggcca cggggggaga
     4501 aggaaggcat tcctcccccg gaaccagcag cacgccggag ctgcgcggag gttccgccac
     4561 cacagcgtgg gccgcacggc cgcaaccgtc aaacacgatg caggagtcac cctgcttcag
     4621 gcgcaaaacc ctggcggcat ggtgcgcttc atcccctcgc agctcccagg cgggagcagc
     4681 ccattcggaa ggcgggagga aaaagcgggc catgtctgaa aaagggaaac ggaccttatt
     4741 tgcccttcag gtaacgcgcc gccttgtcgg caaactccac gcaagccggc tggttccgat
     4801 tttcatccag ggcagaggca aacgcgttca gcttatccat ctgctccttg gtaagccggg
     4861 aggggatttc cacctccacc tccaccagca tatcccccac gtccgaactg cgaagggcct
     4921 ttaccccctt gccgcgcaga cggaagatca tgccgttctg cgttccttcc ggcagcttga
     4981 tggtggccgc gccttccagc gtgggcactt tcagcctgcc gccggaaaca gccagggaaa
     5041 gcggaaccgg aaccgtgcag ctcaggtcgt tgccctccct ctggaaaata tcgtgcggtt
     5101 tgacgtcaat aaacccgtgc agatccccgg tagggccacc atgcaccccg gcatcccctt
     5161 ccccggcgat gcggagctgg ctgcccgtgg caacgcccgc aggaatacgg atggtgatgt
     5221 gggaatcctc cctgacgcgc ccttccccgc ggcagacggg gcatgggtcc gagatgattt
     5281 ccccggtgcc gtggcaggtg gggcaggtgg actgctggac gaaaaagccg ctctgctggg
     5341 taatgatgcc gcgcccctgg caggtaggac aggacttgaa cgcttccttt ccatccctgg
     5401 ccccggtgcc gtggcaggtc ttgcaagtca ccagacgctc aatctcaagc ttcttggtgc
     5461 atcccttcgc agcctcttcc agcgtgatgt ccaaatcata gcgcaaatcg gaaccgggtc
     5521 tcttggtgga ccttttctgg ccgccgcggc cggcgcctcc gaacatatcc gcaaaaccgc
     5581 ccattccgga aaacatctgg gcgaaaatat ccatcggatc ctggaaccct cccccggcat
     5641 aaccgccgcc cgcagcgggg ccgccctgtt caaaggcggc atggccgaag cggtcgtagg
     5701 cagccctctt gtccgcatcg gaaagcacct cataggcctc tcccagctcc ttgaactttt
     5761 cttctgcgga cgggtcgtcc gggttgcggt ccggatggta ctttaaggcc agcttgcgat
     5821 aggccttctt aatctcatcg tcagtagcgt ccttggaaac gccgagaatt tcgtaataat
     5881 cttttttagc catgtggcgt gaatgttaaa aaaacggtca gacttgcggt tcggggtccg
     5941 gagtatgggc gacaaccacg ttcgccgggc gcagaagcct gtctttcagc atatacccct
     6001 tgcggataac gcgcagaacg gtgccttccg gctggtcgga gggttcccgc tgaagagctt
     6061 cctccgtggc gtggtcaaac atgcttccca ccacaggctc cacggcgctc acgccattgc
     6121 cggcgagaaa ttcgtccagc tgcttcttca ccatgctcat gccgatgtaa atcatggagg
     6181 aggcatctgc gctggcggcg gccatgccca tttcaaaatt atcaatgacg ggtagaagtt
     6241 cttccagcag gcgctgattg gcgaacttgg cgcattcctc cttttccttc accatgcgct
     6301 tgcggtagtt atcatattcc gcagcagtgc gcatggcggc atcccgccat ttaagcaatt
     6361 cctcttccag ggaaggttcc gccaccttct cctcggcagg agcatcctgc acnnnnnnnn
     6421 nngacgacgg ccttgttcgc ctttccctcc acgggaggcg cggcaatgcg cagtttcagc
     6481 gcgcggccgg cacggggatc ttcctcccat cccacggcct cgctcttctt cgcattggga
     6541 ataactttca gagccagttt cataccgttt ccatgccgga cttcccgcgc tgcctggcca
     6601 ccagatcccc gtacctgatt aaaccgccgc caaacaaatc caaagcgctc cccaccgtgg
     6661 catccatggt tccgccgctc aaagcgtcta tttcgtcaat atcctgaatg cggctgatgc
     6721 ctccggcata tgtcatgggc aggcggcgcc agtttcccag catttccacc agttcccggt
     6781 caatgccggt gcagagccct tccacatccg ccgcatgaat aagaaactcc gcgcaatgtt
     6841 ccgccagaac atccagcgtc tccgccgtta cccggagttc cgtcaaagtc tgccaccggt
     6901 tcatggccac ggcccagcct ccctgaaccc tgcggcagct caaatccagc accagccgtt
     6961 ccgcgcccac tgcggaaaca agatccttca gcctgtccat ccgcagcgtt ccctccgcat
     7021 caaacaggca ggaagtaaca atcacatggc tggccccggc ggaaatccat tcctccgcat
     7081 tttccggcgt catgccgccg cccacctgaa ggcctcccgg ccatgccgcc agggcctccc
     7141 gcgccgcatg gtcgttccct gggcccagct tgatcacatg ccctccgctc agaccgtccc
     7201 tgcggtacaa atccgcatac catgccgcgc cgcggtcgga gacgaaattg gtgcgcaatg
     7261 agacgccatc ctgcgtcagg gttccgccca caatctgctt cacgcgcccg tcgtgtaaat
     7321 caatgcatgg tctgaatctc gtcacgcggt cactttgccc ggggctcccc tccaggtcaa
     7381 gacggtattc cccttacccc ctccctgaat gacgggcatg gaaaattaca caaaacggaa
     7441 cggggactcc gctctatcgg ataactgccg tcaaccacca ttcaaaaaac cgatgaacaa
     7501 caaccctgcc ggaaaccatc ttcttgcctg ctgcgccgga agcgccatat ctgccctcct
     7561 ggccttttcc tcccccgctg cgaaagccgg gattcacctg catggcgaaa tccgcgtacc
     7621 tgcggtaggg caggccgttt tcctgtatga cccctccgga ttccccatct ggggatattc
     7681 cccatgcgga cggcccattt acgcctatac ccctgcaggc gcacccattt acgtcatcag
     7741 ggacatctac cgcggctgcc atgttcctgc atggaacccc aggccgcatt accacggccc
     7801 tttctggccg gcgggcgtct gc
//
LOCUS       DAWEMX010000119         7730 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3888_length_7730_cov_12.234788, whole genome shotgun sequence.
ACCESSION   DAWEMX010000119 DAWEMX010000000
VERSION     DAWEMX010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_7730_cov_12.234788"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    787..862
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1638 a         2440 c         2105 g         1471 t
ORIGIN      
        1 ggtcaatgac gacaccatgg tccaggtcat ccgcggcggc aatatctgcg aattgccccg
       61 ccgggacgtg gtggtgggcg atatcgtcat cctgaacaca ggggaggagg taccggcgga
      121 cggcgtcctg ctggaagccg tttccctcca ggtcaatgaa tccacactga cgggggaacc
      181 cctcatcggc aaaaccacga atgaagcgga attcaagaag gatgccacct acccctccaa
      241 ccacgtcctg aaaggaacca ccgtggcgga cggccacggc gtcatggaag tgacttccgt
      301 gggtgacaaa acggaatacg gcaaggttta tgaaggttcc cagatcgaca acaaggtgca
      361 gacgcccctc aatcgccagc tttccaggct gggcgatctc atcacctggg ccagctacgc
      421 catcgccgcc ctcatcgtca tcgggcgcct caccctgtat ttttcccatc tgccgggccc
      481 cgtggaatgg ctgagcgcgg gaacctacat tctgaatacc gtcatgattg ccgtgaccgt
      541 catcgtagtc acggtgccgg aagggctgcc catgagcgtc actctgagcc tggcgctgag
      601 catgaagagc atgctcgcca acaacaacct ggtacgcaag atgcacgcct gcgagaccat
      661 gggggccgcc accgtcatct gcacggataa gacgggcacc ctgacgcgca accagatgaa
      721 cgtgtacaag gcggattttt acgggctggg gaacgccgtc ccgacggaag acgagccggg
      781 caacctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnggagggaa tcgccgtcaa ctccaccgct ttcctggatt
      901 acgcagaccc ggaacatatc agggccctgg gcaatcccac ggaaggggcg ctcctcctgt
      961 ggctgcacgg gctgggcgtg aattacctgg acctgcggga aaacgcccgc gtgctggaac
     1021 agctcacttt ctccacggaa cgcaagtaca tggctaccgt agcggaatcc cccctgctgg
     1081 gcaaaaaagt cctttacgtg aaaggcgcgc cggaaatcgt gctgggtctc tgctccaccg
     1141 tcctgactcc ggaaggccag gtacccgccg cggacatgcg ccccgccatt gaggaacagc
     1201 tcctggccta ccagaaccag gccatgcgca cgctgggctt cgcgtaccgc attctggagg
     1261 atgacgcccc ggtgttcgag gacggccgca tcatccgcaa agacctggtt tacctgggca
     1321 tcaccgccat ttccgacccc gtccgggacg atgtgcccca agccgtgaag gactgcatgg
     1381 acgcgggcat ccgcatcaag atcgtcacgg gggacactcc cggcaccgca cgggaaatcg
     1441 gccgccagat cggcctgtgg acggaggagg attctccgga ccgcctgatg acgggagtgg
     1501 aattcgagca gactccggac gcggaactgc tggaccgcgt gatggatctg aaaatcatgt
     1561 gccgcgccag gcccatggat aaggaacggc tggtcaaact gctccagaag aaggaccagg
     1621 tagtggccgt caccggggac ggcaccaacg acgcccccgc tctgaacgcc gcgcaggtgg
     1681 ggctttccat gggagacggc accaccgtag ccaaggaagc cagcgccatc accattctgg
     1741 acaattcctt catgagcatt tcccgcgccg tcatgtgggg gcgttccctg tacaggaata
     1801 tccagcgctt catcgtcttc cagatgacca ttaacgtggc ggcctgcctc attgtgctca
     1861 tcggcgcatt cctgggcacg gaatcccccc tcaccgtcac tcagatgctc tgggtaaatc
     1921 tcatcatgga caccttcgcc gcgctggcgc tggcctccct gccgccggac gaacgcgtga
     1981 tgcaggaccc gccgcgcaaa accacggacc acatcatcac ccgccccatg ggccgaaaca
     2041 tcctgggcgt gggcatcctg ttcgtcgctt tcctgttcgg cctgctgcaa tatttcaagc
     2101 atgcggacat taccagcctc acgcagttca gcctcagcgg ctacttccgc agctacctgg
     2161 atttttccgc cccggaacat gaactgacgg gttatgaact gtccctgttt ttcaccattt
     2221 tcgtcctgct ccaattctgg aacatgttca acgccaaggc attcaacacg caccagagcg
     2281 cctttgcacg catgggcgcc agcattggcg gcttcggtct ggtagccctg cttatcctgg
     2341 ccgggcagta cctcatcgtc acctttggcg gaaagatgtt caacgtggtg cccctgagct
     2401 ggacggactg gggcctcatc ttcgcaggca cctcccttgt cctgtggttc ggagaactgg
     2461 cgcgggcatt cactccggat aaatccggcg cccgctcctg aaccgtccgg gggaattccc
     2521 cccgcgcatg acgcggacgg cgacgccgcc cggaaaacct tctttcaaca aaggcgcccg
     2581 caggcgtgtc attcccagca tggacttgac acgcctgacg ggctctatct ccagccccgc
     2641 cgttttcctc ttcctgcttt ccctcttttt tgccgggtgt aaaaagacgg aacaggttcc
     2701 gcatgaaacg cccacgctta tttatgaaca ccccgtcacc atggatattc cggtgacggg
     2761 cagctgggtg ggccacctgg acggcgtgga caacgcctcc atcgtccccc agattacggg
     2821 ctaccttcaa acgcagaatt acgccaatgg cgctattgtg aagaaggggg aagtactctt
     2881 ccgcattgac gattccacgt tcagggacca ggtatccaag gccaaagcca ccctggccca
     2941 ggcacaggcc cagctacagc agttgaatta cgacgcggaa atttacaggc ccctggctgc
     3001 ggagaatgcc atttccaagc agaaatacga ggatacgcgc ctgtccgccc ttgccgcgga
     3061 agcacaggtg cagcaggcgg cggcggccct ggccctggct gaaaaaaatc tttcgtatac
     3121 ggtgctgtac gcgccttttg acggaatagc cggcatctcc aggacaaaca tcggcgacct
     3181 ggtcagcccg gaaagcggcc cgcttgccgt cgtctcctcc gtcaatccca tccgcgtcaa
     3241 tttcgccgtc acccagcagg aatggattca gcaggcggga aagaacggga atgaaggcgt
     3301 ccagacgggc agcaagctgg acattacgct gaaagacggc accaaatacc cggaacaggc
     3361 cacggtagtt gccattgacc gggcgttcaa tccccagaca ggcaccatcc gcgtacaggc
     3421 caaccttccc aatgcggact atctgctgcg ccccggcatg ttcatcatcg ccacggcccg
     3481 gctggccacc gtcaaaaacg cgctgaccgt tccggcaaaa tcccttcttt cgacacaggg
     3541 gcgctttttc gtgattgttc tggacgggag caaccaccct tccatcatcc ccgtacaggc
     3601 cgggacgcag cttggagacc ggcagcaggt tattcccctg aaaccggatt ccctcacccc
     3661 ccaaagcaaa gtggtcgtgg acggtcttct ccaggcggaa gcagcctcca gaaatccggc
     3721 cgcccgcctg aaagcggtac ccgccagcaa ccaataagct cctcccgcca tggctgattt
     3781 cttcatcaag cgccccatcc tttccatctg cctctccatc atcattgtcc tgctgggggc
     3841 cttttccatt ctgcgcctgc ccatctccga gtacccggac atcatccccc cctccatcca
     3901 ggtgacggcc acttatcccg gagcggactg tgaaacggtg gtcaagtcta tcgcatcccc
     3961 catcgaacag caaatgtccg gcgtggacgg aatgtcctac atgacttccg tcaacaccaa
     4021 caacgggcaa atgagcatga tgatcctttt tgaaatcggc acggaagcca acatggacca
     4081 ggtgctttcc tatctgcgct acgggcaggc cacctcccag ctgccgtcgg aagtcagcgc
     4141 gctgggagtc tccctgcgca agacaagcgg tctgcccgcc ctggtcgtca gcctgtattc
     4201 gccccagggg acttatgacg gcctctggct ggccaattac gcctatatca atatggtgga
     4261 cgccatcaag cgcgtgcccg gagtaggaga cgtgcaggtg ttcggggcag gccgctacgc
     4321 catgcgcatc tggctgaacc cggaaaagat ggccgctctg aatattacgg ccagggacgt
     4381 gatgctggcc gttcaggccc agaacgccgt gaatcctgcg ggcaagatag gcgcacagcc
     4441 cgctccgccg gaccagcagc tttccttcac cgtgaaagct ccaggccgcc tgaccagcgt
     4501 gaaggaattc gaaaatatcg tggtacgcgg gcaggacagt tccatcgtaa gagtgggaga
     4561 tatcgcccgc gtggaactgg gctcggaaac ctacagcctc agttcttccg taaacggcat
     4621 gcccgccgct tccatcggca tttacgaggc gcccggagga aatgccatcc agctggtgga
     4681 caatatcaaa gacctgctga aggatacgga tatgcctccc ggcatggatt acctcgtttc
     4741 cctggattcc acgctggccg tccgcgccgg gattgaagat atcgtcagca cgctggtgat
     4801 tgccctgggc ctggtggtgc tggtggtttt cattttcctc cagggctggc gcggtacgct
     4861 gattccggcc tttgccgttc cggtgtctat cgtgggcgcc ttcatcgcct ttccattatt
     4921 cggtttttcc atcaatacca tctgcctgat ggggctggtg ctggccatcg gcctggtcgt
     4981 ggacgacgcc attgtggtgg tggaagcagt aaagaaccat atttccaatg gagaaaagcc
     5041 cctccaggcc accattgcgg ccatgaagga agtatccggc cccatcgtca gcacggcgct
     5101 ggtgatttcc tgcgtcttta tccccacgct actgttgccc ggcgtcacag gcaaactgtt
     5161 cgaacagttc gccgtgacca tcggcatgtc catcatcatt tccgccttct gcgccctgtc
     5221 cctcagcccc accctgtccg ccgggctgct gagagggggg aaagcggact ccattcccct
     5281 gctggggccc tttttcaaat tgttcaacaa gggtttcaac aaggcgcgcg actggtacgt
     5341 ggaaatctgc gccaggctta tccggcacat gtggctgtcc atcctgctgc tggccgccat
     5401 gaccgccctt ctctttcccc tggccaaact tattcccagc ggattccttc ccaatgaaga
     5461 ccagggctac ctgttcggcg gcgtggaact gccggataat acatccctga acgtcacggc
     5521 agacaccgcc gcgcggattg agaaaatcat cagggaggat ccgggcgtgg acgtagtgac
     5581 taccgtcaac ggcttcaacc tcatcagctc cgtccaaagc tcatccaact ccttcttctt
     5641 catttccctg aaaccgtggt ccgaacggac tgctccgggc atgacggcgg acggcatcgc
     5701 cgcacgcctg aaaagcaggc tgaacgcgga aatttcctcc ggcgtggcgt atgtggttcc
     5761 gcctccgccc ctgcctggcg taggaacgtc cggggatgtc acattcctgc tggaggaccg
     5821 ccagggcatc ggggaaaaat tcctggccgc caacacgtcc aaattcatcg aggcagcgga
     5881 aaagaggccg gaaatagcgg acatcagcaa tttcatgtct ccctccaacc ttcagtacaa
     5941 cctgaatgta aacacggagc aggccactct ccagaatatg gacgtggatg aaatttacgc
     6001 caccatccag gcatacatgg gaagcacctt cctgaacaat ttcaatattt acggacagga
     6061 atggcaggtg tacatgcagg cggacgcacc gtaccgggac agcatcgaca agctttccat
     6121 gttctatgtg cgcaacaatg aaggagaccc ggttcccctg gattccgtaa tcaacgttac
     6181 gcacggctgg gccccggaat tcctgatccg ccagaatatg ttcaacagtt cccagctcaa
     6241 cgtcaccccg gctccgggct attcctccgg ccaggtcatg aacgccctgg aagaagtttt
     6301 tgcccagaac atgccgtccg gcatgggata cgactattcc ggaatgagct accaggaaaa
     6361 acaggcgcag aaaggtatca ccatcggcat gatctttgcg gcatcagccg tgtttgtctt
     6421 tttgattctg gcatccctgt atgagagctg gtccctgccc gtagccgtct tcatgaccgc
     6481 ccccattgcc atcctgggag catttctggc cctgtggata accgggctgg acctgaacat
     6541 ttattcggaa atcggcctca tcgtcctgat tgctctggcg gccaaaaatg ccatcctcat
     6601 cgtggaattc gccgtgctgg aactccgtca gggcacggac ctgctcacgg ccaccctgga
     6661 tgcggccaga atccgcctgc gccccatcct gatgaccagc atcgccttta tcatgggctg
     6721 ccttcctctg gccgtagcca cgggagcggg cgcagcggcg cggcaggtgg taggcatcgg
     6781 ggtcatcggc ggcatgatga cagccgtctt catcggggta tttttcattc cttccttctt
     6841 ctacctgatt gccaaacttg ccaggctgga caaaaaagcg gcgcggaaac tccaggaaac
     6901 ggaacaggga actcccaccc ctgcgaagca ggccgaatga cacttccgaa acgggaaaac
     6961 ggctccctca cagcttccca ttccccctac atccagaata tccaggccca gaaagccttc
     7021 acgaaaacga gcaccacatt aatcagcagc cccaccttca ccatggtcat ctgagggaat
     7081 tttcccgtcg cgaaggccag ggcgttggga ggagtggaaa cggggagcat gaaggcgcag
     7141 gaagcgccta tgcctaccag aagcaccagc ggcgtagcag acatgcccat ggcggaggct
     7201 accgtgacca tcagcggggc caccagggcc gcactggccg tattggaaca gaattccgtc
     7261 agcgacgtga tgaagaggct gatgatgagc aggatgacga tggggctctg ccccatggcg
     7321 agagaagata cctggtccgc caggaatccc gccgcccccg tctggacgag aatgctgctg
     7381 agcgtgatgc ctcccccgaa caggagcagg acgccccagt ccgtattttt ggcgatgccg
     7441 ctccagttga taaccccgca cagcggcagc agcaccgcag cactcagcgc gatgagcgta
     7501 tccatggacg gaatgccgcc caaagcagag gaaaggaaac tgctgcacat ccagctcccg
     7561 gccaccagca cgaagagaat aaggacacgc acctgcctgg cattcaacct gccctccgtt
     7621 tctccccctt ccgccggcgt catatccaca tgcatcccca gacgcggacg gaagaaaaga
     7681 tacatcaggc agaaaacgat cactccgaac accaggacaa tgggcatggc
//
LOCUS       DAWEMX010000120         7630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3951_length_7630_cov_8.451617, whole genome shotgun sequence.
ACCESSION   DAWEMX010000120 DAWEMX010000000
VERSION     DAWEMX010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3951_length_7630_cov_8.451617"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3680..3689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4914..4923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1774 a         2296 c         1907 g         1633 t
ORIGIN      
        1 tccaccacat gaagcgcctg cgcctcccct ccctgcggaa ggtgggcatc ctgatgtggt
       61 cgcgggcctt catgtacgtc cggaaagcag gcaccttcat tcttggagcc tccatcatcc
      121 tgtggtttct gaacacctac cccaggccgg aagaagacac ggcgccgaca aacgcagcgg
      181 ccatggaaca ttcctatgcc ggacaaatag gccattggat ggaacccgtc acgcaagtgg
      241 ccggatttga ctggaaaatc aattccgctc tggtaggagc ctttgccgcc aaggaaattt
      301 tcgtcaccca gatgggtatc ctttatgcgg tggaagacgg aaattcggtc ccggctgccc
      361 agcagacatt gaacgcccgc cttaaagcca gctacacccc tctccagggc atcagcatca
      421 tgatattctg cctgattgcc ctgccctgca tcggcaccgt caccgtcgcc aagagggaag
      481 cgggcacctg gtggttcgct ctggctcaat tcggcggatt gaccctcctg ggttttgcaa
      541 cggctacgct gatctaccag gcagggctcc ttctgtaagg gccagccata aagaaaccgg
      601 aagcagggaa taccccccgg aggaagaaaa aatgaacctc gtggaaaaag aggcgtttac
      661 gatgaacttc cgcaagttct cctgctctac ggtcaagtta tcacaacctc gtcatcatcc
      721 ggccacgaaa gccgcagcat aacaccatgc atggccgtgc aagcctcccc tccgcaacaa
      781 tccggaatca gtatgcatcg gctgaagcct ttcctgtttt tttcaagccg cccgggtatc
      841 cttttccgtt tgcagagggg gggaacaacc tgcggcaaaa aaagatcatg cagccatttc
      901 cggaaaatca atgccctttt gacagggagt aatcaaaaaa ggggcaccaa ttatccattc
      961 ggtaatcttt gccctggata tgggccttaa cctcatctcc ctgggaatcc ctcagacagc
     1021 cccgcgcccc cgtccgcgga ccggaaggac ggtgctcatt aatcgtcatc ccatagcgta
     1081 gacgcccttt cctcgggtcc ttctccgtca acagctgtac cgcattcttc cccaccagcc
     1141 ggacagactt gatggcaata tcctctccct cagcccccac aagggaaaaa ccataattgc
     1201 caggatcgtc aaccgtcttc gtgtccagaa ccaaaggggg gcagggagca taaaatgcca
     1261 cttccaccgt ccaacccttt ccggacttcc tgattttatg ggtcagggga tgcacgggtt
     1321 tccagtccgc cccatccaac aggactttct tcatgacata gcccacatac tccccataaa
     1381 ggcgggacat gggcgccgtc aaatggactc cgtcgctata ctcgaaatga tacatcgggg
     1441 tcgtcatcac ataccggctg tccttctcca aagccatctc cagctgttcc aaggcaatag
     1501 ccggataatc cgtaggatta aactgataat aattcaaatg ggaaccaacc tgatagccga
     1561 aacagaccac atcgtttttc tgcccggtca cggccctggc gtcatgattt atgtccctga
     1621 tcagcttttc cattttttcc cggtaccatt ccctcgtctt tctgtcctgg tgatccgtct
     1681 ctccctgggt ccagataaac gcaggcatgg aaagggtctt cccttcccgg tcagccagta
     1741 ttttggcggc cttcaaatca tccaaaatgc ggttgtaggc tccagttccc ctgcccaggg
     1801 aaccaaggct cgccccaccc actcccgtcg cagaacaaat caaccggata cgggaagccg
     1861 taacgggaga ataatgctgc atcaggcgga acaaacgaca ggcagccccg gatacgggag
     1921 tttcgccgcg cggaccgttc actccttcct ccagaggaac cagcatcttg cggtcactgc
     1981 cgttctcata cgctctcacc cctcccctga acatatacaa gcccccttcc tcctgctttt
     2041 ccgttacaac gggcttagcc gtccagccca cagaaaggga ttgaccgtaa gaagttaaat
     2101 aattccaatc cgcccactct gttttccggg aggcacagat ggatgcggaa gccgccgcag
     2161 ccatgtggaa agcaagcaaa atcaaggtaa caggaatatt catcagataa ttcaacggac
     2221 tctgtccgtg aacatacgtt agccgtcccc tctttttatc aattcctgaa cacaaagctg
     2281 tccatccatg gctgcagtac cattacgcca ggaaatacgg aattgccgtt ccagccgggt
     2341 ccacgccatg tttcggtacc atgccgtacc ggcaggaaaa cgcattcaat ataccgacgg
     2401 gatttttcat cctccaaaag aaaggcggca gaaatattcc ccgtattgta taaaacaaca
     2461 tgaatcattt cccgtctatc aacgccatat ccgcctccat ctgccttctg ggatgcggac
     2521 tcgctctgcc tgttttctcc caacagccgg aaaaaggaaa acccgatgcc gcccacattt
     2581 ccaaaacaaa agctgactgg tggagccaga ggcacgccct gttaaagcag accctggcgg
     2641 aaacaccatg ccaactgctg tttatcggcg attccatcac ccaccgctgg gaaaccgacg
     2701 gcaaaaaaat atggtctcaa tatttttccc cctatgctcc tgtcaatttc ggcataggag
     2761 gcgacaggac ggaacatgta ttgtggcgca ttgacgattc tgcccttaaa acgcctcatt
     2821 ccccccaagt atgcgttatc atggtgggca ccaataacac agggcaatac aaaggcaggc
     2881 agaccccgca ggaaacggcg gagggtatcc gggaaatagc ttcccgggtc catcggctcc
     2941 accccgccac tgaaatcatt cttctgcaca tctttccccg ggggaaaacg gcggaagacc
     3001 ccctgcgcat ccaaaatgaa atgatcaaca gggagctgga caaaacaaat atgccgagag
     3061 tccatgtcgt caacattaat tccgcattcc tggacaagga cggcactttc ctgccgggaa
     3121 ttaccgggga cctcgtccac cttacagaaa aaggctaccg tctctgggcg gacgcactgc
     3181 tgccggaaat caaaaaatac atgaagtaag tccgctaccc ctcccgcccg gctcaacggc
     3241 caattcggcc tcatctgcgc cgaagcggcc agccaatcat ggttgtcatt cccgcacata
     3301 aaaaaccgga gggattattt tccctccggt tttcggatac ggccgttaaa accgtttaga
     3361 ttgtcttaat cacggaagag tcgtcatcat ccctgtcctc ggcagaagcg gccttcctgg
     3421 tgggaacagg cgtcttcctg accttttcca gagccttgtc caccgccgca atcaccttgt
     3481 cttccccggt cttctgactg gacaactcat tataaatctt ttccttggcc tcataatcgg
     3541 ccttggctgc cgcatcgccg ggattggcct tgaacttgtc ctccgccttg aacaaggctt
     3601 cctccgcagc ctggaacttt ggttccaccg cgtttctctg gtcttccgca gtctgcttga
     3661 tgcggcccag caggcgaatn nnnnnnnnnt cacatacggg cggttgctga gcctcttgaa
     3721 tgcggaaacc agaaactggc ggcgttcgct ggttttggca tccgcaataa gggggtcaaa
     3781 caacttgagc gtggcgttgt catcccggtc acggtccacg gagagtacgc ggagaatgga
     3841 atcatgggcc tgggaacgca cgcgttcatg cagggattcg tctttccagg ccttgaacag
     3901 gtacggaacg gcgtcatccg tattccagtc cccccaggcg gtaatgatgg caagcgcctt
     3961 tttgggatct tccttatagc tcttttccat catgtccagc accttgggat caccggtgcg
     4021 ggccagcagc ttgtagagca ttttgacgtt ttcctccggc atgtttttct gatactggag
     4081 cagtttggag gaaagcgcct tgcgcttggc tggaggagct tcgtcaatca ggtagcgcgc
     4141 cgtacggaaa gcggaggaaa tcaaatccgg ggaagcatcc ttcttctgga ggatcttcat
     4201 cacgtcgtca aggtcgtcca gcttcatggc aaaacgcatg tacttgagca ccacggcctg
     4261 cttgtcctgg atcttcttat tcttgctttc gctgatggaa gcgtacaggt ctttcagcat
     4321 gtcgcgcatg gcggggctgg aagacttggc caaaagggtc accatcatgg aatatttttc
     4381 cttactgtac ctgccgacat tttttttcat gtcttccacc acctgacggg caatgttgtc
     4441 atccatctgg gccatgatgg aaaccaggtg ggcggcgccg ggccagtttt ccctgttgcc
     4501 cttgactccc ctcacattgg cgagcagcag agcggcatcc tccgcatcgg gaacgacttt
     4561 cacatccggg acacggctga gattcttgga gtcaaacaga cggctgcggc ccagtttccc
     4621 gccttccacg accagcgcat tcaggcgggc gttctcacct tccacctttt ctttcttgct
     4681 gttggtgctc catgcataaa tccctacgcc ggcagcgatg acaaccaaag cgcatacccc
     4741 ataaataaca ccccgcagca gggatctgga tttatccacg gaagcggcca tatcccgggc
     4801 ggattccgtt ccggcgggag gctgaatacc gccgtcgccc gccggaggag cgccagcggc
     4861 aggggaaact ccctcggcga ccacaggctg gggaaccgca gcaggagcca cggnnnnnnn
     4921 nnnaagctgg tgctgccccc acgggggatt ttaatacttt cagcttggga acggcgcttt
     4981 ccggagcggc gaccggggat ttttccgccg cagcgggagc cgcggccgtc ttgaaaactt
     5041 tcaatttggg agcccccccc gcaggagcag gctccggaac agacggttcc ggggtctgca
     5101 cagccgcagg cgctacccct gcggcctggg ccagagcagc ggcctgagcc tccgccaggg
     5161 ctttctgggc ctccgctacc ttggcaaggg catcctgaat ctgggcgtcc acggaaggct
     5221 cgggctgcgg agcggaagcg gcttcctttt tctccagctc cgccgcaagg cgcgctcttt
     5281 cctcttcggc agctacacgg gcggcctcct tttctgcggc aatgcgcgcg gcttcggcag
     5341 cctcctgctc cgccttgatt cttgcggctt cctgctcggc agctattctc gccgcctcct
     5401 tttcggcagc aatacgggcg gcttcctgct cggcagccgc tttttccgca gcaatcctgt
     5461 ccgcctccgc ctgttccgct gcgcggcggg cggcttcctc ctgctcctga gccagcttgg
     5521 cctgcaactc aggagtgaga aaacgcggct tgacaacctc cacgccttcc gcggcgggag
     5581 aaacaaactt taccttgggt gcggccgcag gggctgccgt taccaaacgg cgtcgcaatt
     5641 tgggaggttc cggcgaagaa ggggttcctg tctgatcggt catcgtgata aaatggctgg
     5701 gaaattggag tgattttata cgattccgcg gccccctgca acctcaaaat accgtctgga
     5761 aaacgggcgg ggaacagccg ccgtgcgtgg ttcaacaggc agattgcgaa aaaaggcttc
     5821 acccgggata taacatggaa aagcgtttaa atatcccgcg tattctcccg caaaatcata
     5881 aaaaacacaa tatgttaaca acaacaatca tgaataaagt aaagcgcccg taatagaact
     5941 tcaagaatac gtaccagtca tttaagaatc ctcttaaaaa atgttataac acatatactt
     6001 ttttcttgcc agaccaagga tgattagtta aataaataac cactcgtcaa catcatgaat
     6061 tccaacgaca gtaccaaaaa taagaggcgc tacacgcctg aagaaaaaca atccgttctt
     6121 gatttcattg ctgaacggaa aacccaaaac aaaaggggtg cccagaaaga agccgccgaa
     6181 cgcttcggca tcagcacggt gaccatctca agctggatga aggcatccaa ggccaagcgc
     6241 ggccgcaagc ccggcaccaa aaatacaact gctcctaaaa cacagcctct cgcagccgcc
     6301 caacccgccg acctgcgccg cctggcggcc gttctggaag aaatcgccga ccttgaagcc
     6361 cagacagcca aactcgacgc cctgcgcgct gaagcggagg aactcaagaa caggctgact
     6421 gccaagcagg cctgattgct tttccggaaa accggcttcc cctgacaagg gaagccggtt
     6481 attttcttta cggagatcgc gcttgccccc ccggaacaat tcctttaata tccgccgtcg
     6541 tgactcaacc cgcccacatt gctcccgcga gtcagtctgt ggaaggcatg tatgccgact
     6601 actttctgga ttacgcctcg tatgtcattc tggaacgggc cgtacccaag atcaatgacg
     6661 ggtttaaacc tgtgcagcgc cgtattctgc acgccatgga ccgcctggac gacgggcgtt
     6721 ataataaagt ggctaatatc gtgggagaca cgatgaaatt ccatccgcac ggcgaccgtt
     6781 ccattgcaga cgcgctggtg gggctgggac aaaaagggct gcttatcgac acgcagggga
     6841 actggggcaa tattctgaca ggcgaccccg cagcggcttc ccgctatatt gaagcccgct
     6901 tcacctcctt tgcgcgtgac gtagtattca gccccaaggt cacggaatgg cagctctcct
     6961 atgacggcag gaacaaggaa ccggtcagcc ttcccgtcaa atttcccctg cttctcgctc
     7021 agggagcgga aggcatcgcc gtggggcttt ccagtaaaat tcttccccac aacttcaatg
     7081 agttgattga agcctccatc gcctacttgc gcggccagcc cttccagctt ctgccggact
     7141 ttccgaccgg aggcgtgatg gatgccacca actaccgtga cggagaacga gggacgggcc
     7201 gcgtccgcat cagggcgcgc atcctcacgg aatcgaagaa acttctccgc atcacggaaa
     7261 tcccgttcgg cgtcaccacg gaaatattaa ttgattccat tgtctccgct gcggaaaagg
     7321 gaaaaattaa aatcgcccgc attgaggaca atacggccca gcatgtggac atcctggtcc
     7381 atctcccggc cggagcggat ccggagcaga cgaggaaagc cctgtttgcc ttctccgcct
     7441 gtgaagtcag catctccccg aatgcctgcg tcattgtgga ggaaaaaccc aggttcatgt
     7501 gtgtcagcga catcctgcgc tacaatacgg actccaccaa ggaaatcctg cgccaggagc
     7561 aggaaatccg actcaaggaa ctgaacgagg cgtggcacca ggcaagcctg gaaaaaatat
     7621 tcattgaaaa
//
LOCUS       DAWEMX010000121         7564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_3993_length_7564_cov_10.660274, whole genome shotgun sequence.
ACCESSION   DAWEMX010000121 DAWEMX010000000
VERSION     DAWEMX010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3993_length_7564_cov_10.660274"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2439..2448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4880..4889
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1605 a         2028 c         2371 g         1540 t
ORIGIN      
        1 gggggcaccc ccagggcggt ggtcaatgtg tacagctcgg agatgaccaa ccagtccgtc
       61 aagctggatc cctatgcgga tgccaaatcc atcgtgacga cgcccaaagg cgtttatacc
      121 tggtctctgt tgggagcgga cggcaggccc tacgctccct ccttggtgct tactccgggg
      181 cccgggtggg tgctggacaa ggatgggcgg atgaccgtcc gcggagaaaa gggcgtcctg
      241 acggaccagt atgacgtgac tgccagctcc accctggaca gggaccccta cggggttcca
      301 tgttccccgg acaatctgaa aaaaagttcc ggttattggg cggaaagcgt ttccggggac
      361 ggccaggggg aaaccctgga aatcaggctc aggaaacccg gccggctgct gggaatcatg
      421 ctggagaccg gcatttcccc ggtgaccgac ccggaaaagg attcggaagc ccggaggcac
      481 ccgaatattg cctactccat gttcagccgc cccaaaggca ttcaggtaac gctgaacggg
      541 acttatactt ttgacgccac gctgcgggat gactggactc cgcagatcgt cgtgcctccg
      601 tattacaggc agcctgtgca taccatcaaa atcaggatta attccattta tcgcggaaca
      661 gggacatcgg acacctacat cggcatcctc aagccgatta tacaataaag aactttccgg
      721 ggctcttttt ttccggaagc gggcgggccg ttttgcgggg attccgtcgg aagcggcggg
      781 gctgggaaga aagattccct ttcaatggcg gaacagggaa ctgcccgtgc ggcccctggg
      841 ataacggcgg aaggggagtg tgagcagata catcagcgcc agcgccggaa cctgcatcca
      901 cagcagatac cagggccatg gcccgagaaa gtccagcacg gaccctccgg cgggagtggc
      961 cacggtaaac ccgtaattgg ttcccaggaa catgttgacg ggatgaatgg ccaccaggta
     1021 cgcattcgcc agcaaaaaag cccacaggaa gtcccagcgc gccggcttcc attccagagc
     1081 cagaggcagg taaagcgccg tgatgactgt cacgccgtgg gaaaggaaaa aggcaaaata
     1141 cgtccatgag gggaaatcat gggccacgga aggagtcacc agcccctgga gggtggcgca
     1201 cagcacgcag taataggcca cggagcgcag ccgccgggag ggtatccaca ggctcagggc
     1261 gcacaccacc tgcatgacgg agcagaaatg cagggggagc atccatgtcc agttcttttc
     1321 cggttcatcg gccagcatgg cggccaggtc gggaaccagg ctgaacaggc aggccgctcc
     1381 cagccagaag gtagtgcggt ttctgacggt tttcttgtag gactgcccca gctccaggat
     1441 gcaggctccc gccgccagga ggacggccag ggccgcccag tgggaaaccc ctgcgaattc
     1501 cagtggaggg acgccattca tgcaaccttc accataacag aaagaagggg gaggggcaac
     1561 gcgaaaagcg ggatgggttt caggcttgca aattcgcctt ctccgggcat acttgcctgc
     1621 ggatgaaaca gggcttgttc atattggtct gcggtttgct ggcggccggc ctgaccgggt
     1681 gcgacgattc cagtgaacgg cgcgcccgtt atgcggaaaa aaagcccgct ccgcgccctc
     1741 tggaaaggac gtttgaccct cccaaaaaag tcctccctcc ttcaaaacct aagaaaaagg
     1801 cccccgtatg gatggattac aagggtgagg acatgcggca gcttacggaa ttatccggcc
     1861 gcaaggtgct ccttgttttt tacgcttcgt ggagccgccc ggccatggat tacgtccagg
     1921 cggtaaaatc ttatgccgaa tcgcaggacg gcaaggcgtt tgccgtgttg attgatgcgg
     1981 atgcctatcc ggacatagct cgcaaatatg gcctggaagc cgtccctctg acggttctgt
     2041 atctggaagg gatgaagctg aaggatatgg tcggcggcat tactgcgccg cgcctgaacg
     2101 aactgattga gcagacgata cgggtgcaat agaagggatg ggctgcggca gcctctgcgc
     2161 ggtgcggggg gcggttgatc ccctgtaaat gaaccttttg tttgttgtgc cttttttgct
     2221 ccgtacctgt aaggacttcc ctttcctgcg tggagaaccg gagaatttcc gttcctgaag
     2281 gtatggattt tatgtgaacg ctgaaaaaac gcaggcagca agccggtaaa accggaacag
     2341 ggaaagggcg acatcattct cctgtgttcc tgtgacggcc attccgcctg caacagcatg
     2401 cggagagaga cgctcttcct tcattcggga attggcagnn nnnnnnnngg atggcgttcc
     2461 tcttcctaga aggggctact ttttttgccg catgaaaaag ccggttcacg ggaacgtgaa
     2521 ccggcgcgga aaaacgagtt gataaatact gaaaaaatca ggctcccagg ttgaacgcct
     2581 tatgtactac ccgggccgct tcctcaatat cggattcgtc caccatcacg gcaatcttga
     2641 tttccgaggt ggaaatcatg ccggtcttga tgttggcatc cgccagggcc ttgaaagcgg
     2701 tggctcccac gcctgagtgg gaacgcatgc cgatgccgac cagggaaagc ttggccagcc
     2761 ccgcttccgt ttccaccttg acggtggagc cgagggcggc catgaccggt ttaagggcgg
     2821 cctgggcgcg gcccagttcg ttggagggca tcgtaaagga ctggcggaca tagccgtcgt
     2881 gggcagtatt ggccagaatc atatccaggt tgatttccgc ttctgccagg gcacccagta
     2941 tctgggccgt gtagccgatt tggtccggaa tgccggtaat ggtgacgcgg gcctggttgc
     3001 ggtcaatgga aatgcctcgg atgacgacgg cttccatgga gggggtttct tcttgcacga
     3061 ttgtaccggg gttgttgttc atggagttgc gaacttcaaa gacgacaccg aattttttgg
     3121 cgaattccac ggaacgcgac tgcatcacct tggacccgtt ggaagccatt tccagcatct
     3181 cgtcatatga aagggtttgt atcttcttgg cgtctttgac cacgcggggg tcacaggtat
     3241 agacgccgtc cacatctgta aaaatctggc acacgtccgc tttcagagcg gccgcgatgg
     3301 cgatggcgga gaggtcggaa cctccgcggc ccagcgtctg gaccattcct tcttccgtaa
     3361 ccccctgaaa gccggcgcag ataaggatgt tgccttcctg caggtacttg ttcatcagcg
     3421 tcgggtcaat gctgtggatg cgcccccgcg tgtggctgcc gaaggtggtg attccggcct
     3481 gcgcccccgt aaaggacatg gctttttcac ccagttcatg cagggccatg cagagcaggg
     3541 cgatggactg ctgttcgccg gtggccatca gcacgtcaag ttcacgttcg caaggcgttt
     3601 cagacaattc tctggcaagc ccgatcagct tgtccgtcac gccgctcatg gcggaaacga
     3661 cggcgaccac ctggttgcct tccctggcgg tttcatggat gcggcgcgct acattgcgga
     3721 tgcggtcaat ggtgccgacg gagctgcccc cgaatttttg aacgataaga gccatgtaac
     3781 aggatgatat gtaggttagg attgaggaag aagatgttct atacgcagga cgcggggctc
     3841 cgccgccacg caggaaatgc gggttatttc ctccagagcc ttctggagct ggccccacgg
     3901 gcaggaatgg aggatgaaaa cgaggtcgtt ccagggggcc ccgccttcat caatatggct
     3961 gggagcggaa gaggtggcgg aaatgccgat gccgaaggtg gccagaatgc gggctatttc
     4021 cgcaatgact cccggctggt ccgctacctg gaagcggacg taatacggag tgaccgtatc
     4081 attgatatgc atgatcccgc aggccttggc atagggattg aagcccgtat ggtattccgg
     4141 atagcggctt tcgcgcatgg cggtgatgat gtcgctgatg acggcggaag cggtggggtt
     4201 cttgcccgct ccgcggccgt aaaatagcgt ttcccccaca atgtccccgt tgacggaaat
     4261 ggcgttgaac acgccgttca cggaagccag gatatgccag tcatggacaa aacagggctg
     4321 gacacgcagt tccagagcgt cttcctgccc ctcatggtag cggatgacga caaggagctt
     4381 gatggtatag cccagttttt tggcaaattc gaaatcccgg ctggtgatat tttcaatgcc
     4441 tctgacgtaa attttatccg gggaaatggt tgttccgtag gccagcatcg ccaggatgag
     4501 cgccttatgg gcggcgtccc agccgttgac gtccagggag gggtcttctt ccgcgaagcc
     4561 cagcttctgg gcctgggcca gggcgtccgc gtaatcggcc ccgtgttccc ccatggcgga
     4621 gagaatatag ttggacgttc cgttgataat tcccacaatg gaattgatgt ggttgcaaat
     4681 cagggaattc tgcaagctct ggataatggg gatgccgccg ccggcggacg cttcgaaata
     4741 aataggggtg cccatttccg cggaaagctt gaaaatctcc gctccgtact cggccagaag
     4801 agccttgttc cccgtaacga cgggcttgcc ggcacggagg gccagcgtca ccaggtcgta
     4861 agcctggcgc gttccgccan nnnnnnnnnt tccaggttgc gcacggcgat gcgcttgagc
     4921 ctgaaaggaa tcttgctccg ggcttccaga agggcgtgat tgcggcacag cgtttcataa
     4981 acgccggaac cgaccgttcc cagcccggca agcccaagtt gtataggttt ttccgtcatt
     5041 cctgccgggg gattatgcaa aaaaatccct gtaacgcaag agcttttacc gttccgggat
     5101 gccgtgtgtg cgatgctcat ggaataccct tctttccggc atttccctcc acccccgcat
     5161 gttccatgcg cggcaggacg attgctggat tttcaagaat ctgtgcggat gatgggggaa
     5221 tggcgttcaa gcttgctccg ggagaggatg aatcctgaaa aaaggcaaca aaaaaccctc
     5281 cttgaaggag ggttggattg gcagccccac caggactcga acctagaaat actgaaccaa
     5341 aatcaggtgt gttaccaatt acactatggg gctgtctgct caggcgtttc tgccgttgcg
     5401 gggcgtagta aaccacacgg ggcgcagtct ggcaagagaa aaatgccctg tctcccttgt
     5461 ttgaaggtca atgccccggc ttgatgatgc gtgtatagct caaccgtttg aacatcagca
     5521 tgggtatggc tactccgata accagggaaa agatgatgaa gaaggcggtg atgccgtttt
     5581 tgcagatgtc cacaatcaga cggttcatga cggccgcgtc ctggttctcc tgcatgaact
     5641 gcatcagggc caggatggtc ttataggcgt acacgccggg aatcatgggc aggagggaag
     5701 ggaaagcgaa tatttccgcc gggcacttca ccagcttggc cgtcatgacg cccagcatgc
     5761 ctatggtgaa ggcggcaatg aaggtggcgc tggaaatgtc aatggtccag gagtgcagca
     5821 tgtaaaagcg gaaagcgtgg ccgacggccg ccagaacggc ggaaacggca atggcgcgcc
     5881 tgggcggatt ggagatgacg gcaaatcccg tggcggcaat ggcggccatc agcccgtcca
     5941 gaaggatgga ggaaataaga tccggattca taagctgttt acccctacga tgagcatggt
     6001 tgcggagagg ccgatggcga tgcaggcaat catcatgatg gaatggatgc cgcgggagat
     6061 gcccatcagg atgtggccgt gcatcaggtc catgatggag ttgatcatct ggacgccggg
     6121 caccaggaac aggacgctgg ctgccagggc gatctgcggc gtggagccta tctggaaaag
     6181 gacggcgcac gcggctgcca tggaagcggt gaaggcagaa aggatgatga cccccagcgg
     6241 gttgtatttc aaccgggtaa gctgctgctt ggccagaaag cccagcatgg tggcggcgaa
     6301 cacggtcagc atggcccaca ggtctccgtt aaacaggcgg cagaaggcgg cgttgcccac
     6361 gctgaccagg agcaggtccg tccagcggga aaatcttttg gtgcgcacca tggagcggaa
     6421 tgcctgttcc atttccgcaa tgctgacgtt gttgtccacc gggagccagc tcaattcact
     6481 gagctgctgg atcaggttca ggttgaacgc caggggtttc agtttccgga cggaggtgcg
     6541 gcgcagcgtc tcgtcatcct tgcagatcag gctcatggtg atattgcgct ggaaaatggt
     6601 catgtccgcg ctgtatccgt acgcggcggc aatgcgggaa atgttgcgca cggcgcggtt
     6661 ggtgtgcacc cccgcgccca tgagggtggt ggcgtattcc agcaggaatt ccgaaagggc
     6721 ctgtatctgg tcggtatgct gcataaaagg gaaaggggag gtcagggccg ggatgcccgt
     6781 tttctggagg cgtaatcctc ctgctggtct tccccggtga cgcccacgga gaaataagtg
     6841 ctttccggat gggagaagac gagggcgcag acggaagaaa cgggctgcat catccatgtt
     6901 tccgtcaggc tcatgcccgc ttcctcccgg gcgtggagca gggtgaagac ggtttccttt
     6961 tcctggtggt ccggctggct ggggtacccg caggcgggtc gcacgccggc cccttccgga
     7021 atattccaca aaaggcgcag cttgtcatgg gcgatggccg cgaaagcctc caccaggcgg
     7081 tccgccagag agctgaccag cagggcgcgg taggagtcgt ttctggcttc ccattccttt
     7141 gcccagcggc gggagccgtg gatgctgacc gccatggccc ccacgtaatc cctgacgcct
     7201 tcgggagcga tgaagtccga cagcgccagc cggggtttcc cctgtttgtc ctgttgctgg
     7261 cgctgcgtca gcagcgtagc caggggaatg gttctggatt catcagccca gacggtgatg
     7321 tcgtcatggc tggctgttga attggccggg aaaatgccta tgacgccgcg cgcctggtag
     7381 cggttttcct tcacggcctg ttccagcagg cggagggcgt cctggtacag cgccgcggcc
     7441 gcctcagcct tggacgggtc tttggtgcgg aactcccggc gggcgcggtc ccatgtgccg
     7501 tgcagctccc aggcgtggaa aaaaggcgtc cagtccatgc gctcgataag ctcctgaaca
     7561 gtta
//
LOCUS       DAWEMX010000122         7403 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4091_length_7403_cov_13.806886, whole genome shotgun sequence.
ACCESSION   DAWEMX010000122 DAWEMX010000000
VERSION     DAWEMX010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_7403_cov_13.806886"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1378 a         2026 c         2428 g         1571 t
ORIGIN      
        1 gtgcgtgatg gagatcacct ggtgcctgtt tccgagctgc tgcatcttga atcccacagc
       61 ccgcgccact tctccgccca cgttggcgtc tatttcgtca aataccagca ggggggtggc
      121 atccttgtgg gccagggcgc ttttaatcgc cagcatgatg cgcgccagtt ccccgctgga
      181 ggcaatcaga cggagaggct tggagggttc tccgggattg gggccaaaca ggaattccac
      241 cgtttccatt ccctgtgagc ccggttcctg aaggggggag aggctcactt caaagacggc
      301 ctggcggaat cccagctccc gggagtgcct gacaatggaa gccgccagcc tgggcgcgga
      361 gtcctggcgc gctctggtca gagcctgccc ggcggcgtcc atctgcttgc gcaggacggc
      421 gatggaggcc ctcagctctt ccaggcgttc cgtgcggtgt tcaatgcggt ccaggcggct
      481 ggccgcctcc tccctccggg agcagacgtc ctcaaaggag gggccgtatt tcattttcag
      541 agattccagc aggttgatgc gttcctccag ctggaaaagt tcccgggggt cgcagtccag
      601 ttcggcggaa taatccgcca ggcgtccctc aatttccttg agttccaaat tcactcccgc
      661 caatggagcc agccacgcgg cggtggaggc gtccatgcgt tccagctcat gggccgcccg
      721 tgtcaattcc ctcaattggg tccccaggcc gggcacatcc gtttcctcca gcatggaaag
      781 cattttggag acctgttccc ggagccgggt gccgttgcgg gcgcgctgcc agcgttcttc
      841 cagcgtgagc acttcttccg gagtgaaggc ggcggaatct atttcatcca cctggtggcg
      901 cagcagttct atttcccgtg cggtggccgc ttccgcatgt tccaggtcat cataggcccg
      961 gcgcgcatcc tgccattgcc tccaggcgtc cgaataggcg tgcaccaggg gagcgtgctc
     1021 cccgaaggcg tccagcaggg aaagctgcct ttcctgggag accagggagc ggtggtcgct
     1081 ggggccgtgc atgtccacca ggcaggctcc cgcttccctc agcagattca gggtgcacgg
     1141 gctgtcgttc aggaactggc ggttggccgt ggaagagatg aggcgcttga taatcaggtt
     1201 gccgtcttcg cagggcggga cgccatgctc gttcagaatg gcgtgcacgg gggaggattc
     1261 cggcagatgg aacacagctt ccacgctgca ttgctgttcc ccggagcgga tgagcgtctt
     1321 gtccgcccgt tctcccagcg ccaggcgtat ggctccaata atgacggatt tccccgcccc
     1381 ggtttccccc gtgatgcaga tgaatccgga actgggttcc cagagcagct ggtccacaag
     1441 ggccaggttt ttaattttca gcaatgtaag cattcgggct ttggattttg aatgcattat
     1501 ggcatcattt gccctaaacg caatcatgac ctctgtgatg aacattctct ttttgggcac
     1561 aggaacgtcc accggagtgc cgcagatagg ctgctcctgc gcggtctgca cgtccccgga
     1621 ccccagaaac aggcggttgc gttcttcaat ttatgtggag gccgctggca cccgcctcct
     1681 gctggattct tccccggatt tgcggcagca ggccctgcgg gaaaatatca cggatgtgga
     1741 tgccgttctt tatacgcatg cccatgtgga ccacgtggga ggttttgacg atttgcgcgc
     1801 cttctgctgg cgccggtccg gcggcctgcc catgtatgct tctcccatga cggtggacgc
     1861 cctgcggacc atgtacggct gggcgttcgt tcctaaaccg gggcggagcg ggtacgtcag
     1921 gccggagcct cacgaggtga cggctccgtt ccgtgtgggg aacgtgctgg cgacgcccct
     1981 tccggtgctc catgccgggg tggagacata tgcctatctc ctggaagcgg aaggccggag
     2041 cctggtttac atgccggatg tgaaaagcat cccggccccg tccctggaga ggatgaaagg
     2101 agtggatttg ctgattattg acggattgcg ttatcatctg caccccacgc acatgtgcct
     2161 ggaggaaacg ttggccgcca ttgccgccgt ccggccccgc cgcgccgtgc tgacccacct
     2221 gtcccatgat atggattacg ggattctttc cggcaagctg ccggaaaacg tcatgcccgc
     2281 gtatgacggg ctgagactgt ccctgccgta atgctcacgc tgcatgttct caggccatga
     2341 ctattccaga caattctgac cgtcccggcg cgcggatacc gtgcgaaacc agaaacgtat
     2401 tcggcttttc cgtagccgtc agttccgtgg aagagatgtc cgctgcgctg gcggaacgcg
     2461 cgctggaggc ggaagctccg tttctggtgg ccgcggcgga tgcccatgtg gtaaccctcg
     2521 gcgtgcatga ccgggattac gggaacgtgc tggaacggat ggacgtgatt tgcccggacg
     2581 gtatgccggt agtgtggagg ctgaaccgga ggctttcttc cggggaaagg gaagcgtgcc
     2641 gtgtgagcgg gccggatttg atggaagcgc tggtgcgttc gaatgtgcag tatccgggtt
     2701 tgcgccattt tttgctgggc ggggatgaga agctgctgga agccctgtct ggggcattaa
     2761 aggaaaaata ccccggtttt caactggccg gggcgtattc ccctccgttc cgcccttgga
     2821 cggaggaaga tctggaaaac atgcgggagg cggttgcttc aagcggggcc aatgtggtat
     2881 gggtggggct gggctgtccg aaacaggagc ggtggatggc ggagcagagg gaattgctgc
     2941 cccccgcggt ttatgtgggc gtaggggcgg cgttcgcttt ccatgccggt acggtgaaac
     3001 gggctccgcg atggatgcag aagaacggtc tggaatggtt gtaccgcatt taccgggagc
     3061 cgggaagact gctcaggcgg tacgtgaagc acaacagcct gttcgtgtgg tacgtgctga
     3121 cgggcagata gggccgtttt tccggtgtgc gtcccggagt agagccgcgg gttattttat
     3181 ccttgtgccg ttttcgtgcg gccctatcat gattccgttt gaacctgaac ggaagtggtg
     3241 ggcgctcttc cctccttcgt ctttaacctt gtttttctta tgttctttcg ccgtattccc
     3301 cggaaaaact ggtatgaaaa aactgtggaa cgcgttttca gggacaggaa gctgtgcgtg
     3361 gaaaagctgc tctccttcgg tttcgttcgt gtggaaagcg gttttctgcg cagggcggca
     3421 ttgctggacg gccagttctg catggaattg gaaatacacg cggatggttc tgttcatgca
     3481 acggtacatg atgcggacgg gaagaatatc cggcatgctg acccgggaac ggaggatagg
     3541 ttgcggacaa gaatgctccg cagggaatat gaggaagaac tgtggcatgt ggcggaatgc
     3601 tgctttgaac ctgatttttt caaggcggct cccgcccgga gcctcatcgc gcacatccgt
     3661 aaagcttacg gggaggaact ggaattcctg tggaggaagt ttccggggaa cgcagtggtg
     3721 cggcggaagg atacggaaaa atggtacgcc gcttttctgg ctgtgccgcg cttgaagctg
     3781 gggggcagtt ccaaggaacg ggtggaggtt ttgaacctgc gggtttgtcc cggtgagtcc
     3841 gggattcttg cggataatcg cagccgtttt ccggcctacc atatgaataa gaaaaactgg
     3901 gtgagcttct gtctggatgg aaccgttccc tttgaggaac tggctgcgcg cctggaaacc
     3961 agccggaggc ttgccggaaa gtgagcgcct ccggcgcggg agtgtcggga gaaactgtct
     4021 tcccgccccc ggatggacgg ccggaaaggg ggtcttgacc cggaaagagg cggagcctac
     4081 agtaagggca tgaacataga agaagaagct ctgtccacct tccgcacggc gccctggcgc
     4141 cacaactgcg cccaggccgt ctgcgcggcc ctgggcaggg aggatttgct ggaaaccgtt
     4201 tcctcctgcg gtaccggaaa ggctccagac ggactgtgcg gcgcccttta cggggcgctg
     4261 ctgtgtacgc ccgtgcagtc cagggaggag ctgaggcggc ggtttgtgga aaagctgggc
     4321 tattcccatt gcagggagtt gaaaaaagag ggggccgtgc catgccggga ttgcgttgcc
     4381 tctgcggcgg ttctggcttt taacctgagg gagtgaacgc agaatgacac tgccggattc
     4441 atggtttttc atgaaaaaag agtctattgg aatgaaacca gatagaataa catcatgaaa
     4501 aaattgatct atatgatagc ggcagcggcc gttgcgattc cggtggtggc cctggcccag
     4561 ggagcctgcg gcagcggctc ctgctccaag ggagacaagg acatggctga agccaaagag
     4621 cacatgaaag agggcgccaa ggctgccaag gacgccgcct ccgaaaaaat gaaggaaggc
     4681 aggcaggctg ccagggacgc ctacgaaaag ggcaaggaaa ccgccaggga tgcttatcag
     4741 gacagcaggg acgccgttga ccatgcggcg gacagggctg ccgataaggc cgaagccgtc
     4801 ggtgacgctg tgaaaaacgg ttaattcaaa cgatgaacaa taaaaatatt tgcttattat
     4861 gtgcgaatcc aaggatcata tgaataaggg ctgcggatgt gcgaacgatg ccgcttccgg
     4921 caaaatggag gaaggcaagc acaagatgaa agacgcctgc gaaagaggca aggaagccgt
     4981 caaggacatg gctgaaagcg tgagcgacaa ggccaaatcc atgatggaca aggataagaa
     5041 ttcttagtcc cggcggcagt tgaagcaagc cgtttcccat tcttcatccc gtacgcggga
     5101 tgacctcccc ttgtccggat ttccggacga ggggattttt atgcgggccg gttttccggg
     5161 gaaggggtgt cctgttccac ggtctggagg gcatgcaggc tgattttatg ccttttgcat
     5221 aattcctcca cccggtcgcg ttccagcaag atggttttgc ccgcttccag ggcgatttgc
     5281 ctgatgccgc attccgcgca cgtttcaatg gtgacggggc cgactgtcgg gatgtcgaag
     5341 cgcatgtcgt gccccaggcg ggccactttg gccagcgtag ccggtttgcc gttgcccagt
     5401 tccccaccgc ggcgtatgca gttgttggtg ccttcaatcg cttccacggc gatgacggtg
     5461 ccgccgtgca cgatgacgga ctgcccgatg tggagacggc tgatttcctt ggccgtctgc
     5521 atgccgaagc gggcatcctc ccattgttca ggcgtgggag gcggtccggc gatatgcccc
     5581 ggctgcggca tgtgctcctc catgtaggtg gaggccggga ggatggtgaa gccttccttt
     5641 tccgcttccg tgatgacggc cccaagcagg gagtccgcat ttttttccgg catgcgcatc
     5701 aatacggaaa gggcgcgaag gtccggacgg agggaaagaa tgttcttcgg attgatgccg
     5761 ccggccatga tgacgttcct tacgccgtgc tttttcagga aagtgaacgg cttgcttatc
     5821 tggcccacgc tgaattcctg gtaagcgtcg cacaggggga tgacggcggg gttggtttcc
     5881 cccttgaacc ctacggccac gatacgcaat tccggcgtcc ggcggcgtgc gccccggaca
     5941 atatattccg gataaacgcc gtctccggct accaggccta atacgggcgg gtctgttgtc
     6001 atgcggcgtg aagctgggga acggggctgg ttacaggtat tccgggcgct ggagcaattc
     6061 cgccacgcgc ttgagcaggc ggccgatgtc tgctccgtcc accacgcggt ggtctcccgt
     6121 agccacgatg ttggccttgg agatggggat aaacgcctcc acttcgtcac tccagaccgg
     6181 ggttttggtg acggccccca cccctaggat aatggattcg ctgggcatgg gcataggtgc
     6241 ggcaaaggtg aggccgaagc cgccgaagtt ggtgaccgtg gcgatgccgc ccgtgctgtc
     6301 ttccggagcc agcctgcggc ggcgcgcctg ggcaatcagc ctgttatagt cttccagcag
     6361 ttcttccatc gtccgttcgt tcacgcggcg tagtacggga accatgacgc cgtccgccac
     6421 ctgggcggcg atgccgatgt cgattgtttt gggagaaaga atgttttccc ccaccaggta
     6481 gccggcgcat tccgggcttt ccgccagggc cagggccagg gcgcgggcaa agtacagggt
     6541 gatgccgggc cgcagcgggg aattttgcct gtgcttgatg agggggtcca tgaatacggg
     6601 acgcccggcg gaggccagcg ggcgcgtcca gctgcggcgc atggcgtccg ccacggccag
     6661 ccgcatggat gaggccttgc ggtgcggcca ttggctgaca tattccagga attcttccag
     6721 gtcgtcaatg gtgacgcgtc cgcccgcccc ggaaccggag atgaaagcga tgtcggaggc
     6781 gctcatgccc aattcatcca tgcgcgcctt catccggggg gacatgtagt gcgcgccctt
     6841 catgccggcg ggtacgggga ggccgcgcac gctgggctgg actttcaggt ccgttccgtt
     6901 ttcctgatag gattcccccg tcaccccgaa gtggacgcct gcgggttttt cctcccgggg
     6961 aggggcggag ggcgcggcct ggggaacgga ttccggactg gctggaaggg acggctgggt
     7021 ggaatctccg gccggagtcg ctccggaacg ctcaatttcc tcttccgtgg cctcaatcat
     7081 ggccatgcag gcgccgacca cgacggattc cccttccttg atgaacacat cgctcaggat
     7141 gccgccgcac atggtggtga cccccatggt ggccttgttg gtttctacct caaagatttc
     7201 ctggtcggct tctacggtat cgccctgggc cgccagcagg cgcagcacag tggcttccgc
     7261 aatggaatcc ccgagctggg gcatcagaat gggtacttta ggcatgatgg aaactgggtt
     7321 gaatcaaatg gatagaagat ggcggatgga ggcggcaatg gattccagcg tggggcggtg
     7381 cgccttccag aggttggggt gct
//
LOCUS       DAWEMX010000123         7235 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4199_length_7235_cov_12.153064, whole genome shotgun sequence.
ACCESSION   DAWEMX010000123 DAWEMX010000000
VERSION     DAWEMX010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_7235_cov_12.153064"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1773 a         2172 c         1828 g         1452 t
ORIGIN      
        1 gccatgggaa ttctgggtgc cgttgaacac ctgctggtca ggttcccgga cacccacgtc
       61 ctcctgctgg gcattttccc gcgcggcaca ggtccccagg acaagctgcg ccagcacaac
      121 gaccgcatca acgccattct ggcccagtgc aagctgcccc gcaccactta cgccaacatc
      181 aacaggcgct ttctggactc ctccggcaaa ctgctccccg gcatcagcag ggacaacctg
      241 cactttacgg aaaaaggcta cgccatctgg gcggacgccg tgctgcccta tatcaaaaaa
      301 tactgcaaat agcattcttt cccatcctca acgcatgact gtcggaatgc ccccttccgc
      361 cagtcatgcg tgcggttcgg gaacatattt gtcaatcaat gatgaagctt acgcagcaac
      421 aactgcccag ccttcattct tgaaactgcg gtcatggagc ctgaaaagca ccgctcatgt
      481 ccatcaattc atttttcccg gcggctccgc cttgaatgct tatgagtaac gtccggactt
      541 tgggtcgccc gctttaataa acggttaaga tataaaagca gtacggtgca tggaaagtat
      601 ttaccgccaa gcccgcaaaa agctgcatgg atgccagggc tctcgtctga aaattttgcg
      661 cagaaccgaa gttgcagttg atttggaata aatatattat aatgagacaa nnnnnnnnnn
      721 atgagacaat atatcataga cgcttttacc gacaaagttt ttcatggaaa tcccgcggcg
      781 atttgcattt tagaacaatg gccgtccggg gaattgatgt taaatatggc cgttgaaaac
      841 aatctttcag aaacggcctt tgctgtcaaa aaggggggga gctatcatct gcggtggttt
      901 actcccggtg gagagattga cttatgcggt catgcgacat tagcctgtgc ctatgttatt
      961 ttgaacttct atgaaaaaga agcggcaaaa gttgtttttc aaactttaag cggtgatttg
     1021 gcggtagaac ggcatggtga tctttatgaa atggatttcc ccgcctatga attaaaatca
     1081 gttcccgtaa cggagcaaat gatcgacgct atcggcgcta cccccagaga agcgtatatg
     1141 gggcgtgacc ttttatgcgt gtttgacgat gtaaaaataa tcgaacatct caatccggag
     1201 ctatcaaaag ttcatgccct gaacggtctt ttgctccatg taaccgcaaa aggtgcggaa
     1261 actgactgcg tttcccgtac ctttgccccc aaatgcggag tgccggagga tgccgtttgt
     1321 ggttccggtc attgtcacat tataccttac tgggcgaaca gattgggaaa agacgacatc
     1381 atagcttatc aggcttccaa acgcggtgga acactttatt gccgcatgga tggagcacgg
     1441 gtaaaattag caggaaaagc agccttatat tccattgccg aaacacaatc aagatgaagc
     1501 gtcaataggt taagaacata tgccgggcga cgggaaaaaa cgttgttcga cagaaattat
     1561 acatgcccaa tgacacaaac ggcggttttg aacaatcaag gccgccgttc ttctattccc
     1621 taaaaaataa tgcaactatg ctttcaaaca tggcggtcaa cgggaataac tgccatgaag
     1681 cacaaagcat gccgacaaat tccgacgagg tctatatcaa aaaatacatc cgccgcagaa
     1741 tatcatatct ccaaacgtga atcataccat gtagcaaaag tgaatatttt ttcaaacttc
     1801 catccggtat gcttgtgtct ttacaggtgt gttttttata ggaaatacaa caaaaaatca
     1861 tgaaacaacc aaacctctcg gccggaagca ggaatccagc cgacccgcct gtgccgcatt
     1921 tggaatcagg aaaaagcgca aggaacaaaa aaattgcttt ttcccgggga tatcctagag
     1981 tgcctctccg caatgagcat gccgcttgcc gacttgatct tcctggcttt tgccctctcc
     2041 gtggacgctt tcgtcgtggc gttctcctac ggcctgctca ttaagcaaaa gcgtttgagc
     2101 aacggaatca agctctccct gtccaccggg gccggtcaat tcctgatgcc cgtcctgggt
     2161 tttctgctta caggctccgt gcaccggtat attgcggcct gggaccactg gctgggcttc
     2221 gccgtgttca ccttcctcgg agtcaacgtc ctccgcgaag gctggaatca ccgcaacgaa
     2281 gaagaggata tgccccacgt caccagcctg acgcttccta cccttatggc cgtgggaatc
     2341 gccaccagca tcgacgcgct ggtagcgggc atcagcattt acctttcatc cgcccagtgt
     2401 ggaaccgacc ctaccctcca cgcggtgctt ctccccgccg ctgccatcgg cttcaccacc
     2461 ttcctgtgca cggcggccgg ctttttcctg accagacggc tgcaccgctt tccgaccttt
     2521 catctggaag cgggagccgg gctcatcctc atcggcctgg gcgtcaaaat gctctgtgac
     2581 catttgtgct gaaatatgcc gcgaaggatg ataaaaacgt ccttccggca tcgtaataag
     2641 tcgaaaaacg cactggttcc ctcccccctc tttctgctat tatcaggtaa tcccttccac
     2701 atgaatattt cacatcttct tctctgcact tccctgctcg cggcgcccgt ctgcgcggcg
     2761 cagggcccga cggaaactga aacggaaact ccggcctcct ccagccgcct gccggcgccc
     2821 ctggcacaaa aaattgcctc cggagacttt gatggcctgc agacggaact gcgctccacc
     2881 ctcctgaagg ctggagaaca aacgaaatcc gaacagaaac tgctccagaa caagcagtac
     2941 cgccatttgc tggacattca cgaactcctg cgcgtcacgg gaccggacaa ggtgaaagcc
     3001 gttttctcca aaagcgcgca agatgcagct ttcatcaaaa cctttctaca ggacccggcg
     3061 tggatggagc tttaccttgg agcaggcctg attccggaaa attcaccgga aggccttcaa
     3121 atcctctccg acatctggaa agcggacggc aagagcccgg atttccggaa ttaccaatcc
     3181 ctagccaccg ggctggcctc tgtcttttcc accggcccca tggcgggaag gctcaaaacc
     3241 aactccgcca acagcaatcc ggtgcgccgc taccggattt tcaaaaaact gcatcaggaa
     3301 aacaggctgc atccgggatt catcaaactg cgcccatggg aaatgcgctt cgtcgtggga
     3361 agtccctggg acgataaatc ctatgaatgg agcaatgaac acgtcaacct cccctggcgg
     3421 cgatacacgg ccgcctgctg ggccgctccc tatacgggcc acaatttctt tggcgatacc
     3481 attcaggggc cccttttcta cgtgccgtgg agggatctta acacgaccgc ggaaaacacg
     3541 cagatcatcg gcggcgtttg cggcggcctc tcctacttcg gcaccatggc ggcccaggcc
     3601 cacggcatcc ccgcttatcc ggtaggacaa ccgggccact gcgcgtacgc ggtgcgcgtg
     3661 aagcgggggg aatggaaagg cggcttcggc ggaccagacg gaggcatgca caaccatatc
     3721 ttcggctccc aggctccgac ttcctacctg ctgatggaaa atgtctttgc tgacaacgat
     3781 aaggcggacc aggcctacct gtgggccgcc caggcccggc tggacgaggc ttccggcaac
     3841 aaggacaagg ccattcaggc atgggaggaa gcgctcaggc aaacaccgct gcacccgttc
     3901 ttccgcacgg agcttcaacg cctgctgatg gaaaaggaag gcatgcaacc tgtcgactgg
     3961 tacgtttacg ccaaggacgc cctttcccac tatcagggca acggctttgc cgcattcgac
     4021 atcctcaagg acgtgcagaa taaattcctg atggatattc cgcccgcaga ccggattgcc
     4081 tggttccggg acctgcatga agccatcgcc accactccca catcatgggc ggtgaaattc
     4141 cagcccgtgc tggactccca atctgccttc ctggcaaatc cgcaggaaaa agcggcctat
     4201 ctggaaacag tcctctccac ccacctgaaa acgggggacg gcaccaactt tggccaggcg
     4261 ttggaatggg gcgtgaaaaa ctttgtggaa aacggacagg cggacgtgtt ctccaacgca
     4321 ttcgccaagg tagcccagca aacgggaaaa accgggacct ccgggaaagc ccccgacccc
     4381 aaaaagctga aggaagccta cggcaaagcc atctatgcca cggaaacggc ccgctccatt
     4441 ccggccttcc aggccttgag caaagcggcg gcctctttct ccggcgccaa tgcgacgaac
     4501 aatacggtca aggcgtccat tccgcagggc tggaaactgg tgcccgcaga cggcatggtc
     4561 cggtgctcca ccacgagcca gtgggacagc ccctgggacc atatcaacct gctgcgtccc
     4621 tgcggcggag cccagcatac ggataaggaa gccaatccga acgtcattgt agaactgaaa
     4681 aacggagtga acctggccgg gctggtagtc accaagcgcg acggcaatga aaaccggatg
     4741 aagaaaatgg aggtctccac gtccacggac ggagccacct ggttccccct ggccgccacg
     4801 gaaaacatgc ccaaggaatg ggtcatcacc gcaccggaag gcaccaaagc caagtggatc
     4861 aaggtggaag ccaaaaacgc acagccggaa ttcatgcacc tgcgccatat cctcgtttac
     4921 gaaaaataaa ttctccatta acgccatgct tcatcacatc aatcccgttt ccggcctgct
     4981 ggccgcagcc ctcttcctgt ccgcccccgt ccaggcggcc ctccccgctt acagcgccgt
     5041 gaaagacgag gcgaaaaccg tcaataaata catgatcgtc gtatgggccg gcacggactg
     5101 gtccccaaaa tcacgtgaaa tcacccgcgc cgtggaacac ctggccaaga actccccgga
     5161 acccgtgctc tggtgtatcc aggatgagcg tgaggaaatg acggaagaag agcagaaaca
     5221 gcccaagccc ccgggggaaa tctggaacat ccccgccatt caggtggtct cccccaccgg
     5281 aaacatggta ttcctgtcgg aaggcgtctc cagggaaaca ttgccagccg tcatgaagca
     5341 ggcaatggaa gccgtaaaac agcaaaacaa ggccaatgcc ctgtgggaaa aagccgccgc
     5401 ttcatccgga acggccgcgg cccttcttta cggagaaggg cttcagcagc ttcctcctta
     5461 tgcggcctct gccagaaagg atattctgga aaaaatcaag aaggcggatc cggaagacat
     5521 caagggagtg cacttcaaat acacgttcag gcatctgccc tacatcgaaa aagtgcagag
     5581 aatggtgaat gattccgcca aagacggcgg tccaaaggat tacaaaaccg cccatgccta
     5641 tgtaaacaaa cagctcaaaa cccccggcct gactcccctt caaaaacaac aggtcatggc
     5701 ggccaggttc tggctctacc gcaatgaggg gaaaaaggac caggcgctga agaccctgac
     5761 ggacatcgcc aggatatccc ctaaaaccct catgggaata ggcgcccaga actactaccg
     5821 ctacctgacg gaacccgtca ccctgaaaag tcctcacttc acaggctacg acctgcgccc
     5881 ggaactgacg cttacccgcg tcaacgtcag cagcatgctt gacggccccg gaaactacaa
     5941 gattaccttc aagatgaaca gcggcggctg caatatccgc aacccccgct tcatgaaggg
     6001 caaccgcgta gtcagcgaac tgcccaagga ccggcaggac aaaaacgggc gcgaattcac
     6061 cctgcgcctc tccggttccg aaaagccgga cttggttttc gactgccagg gccagggatg
     6121 gttcgatgcg gactgcgaca tcatcgtgac caaggaatcc tgactcctgc cggcaaacga
     6181 acaaaaccgc cattcacaac cttcacgaag gcaggacatt cccaaggaat ttcctgcctt
     6241 ttttccgatg ggagaagaat ttccagccag cgcccaagcg gaagcatccc tctctccctg
     6301 cttcttgccg gccaaagccg aacaacattc ccggcagctt caaaggagca atcagggaaa
     6361 cgttcttctc attcccgcgt tttcaaggaa gaattacccc ttcttcccgc cttccccggc
     6421 gttctccgcc agttgacggc ggtattccat gcgttccctg atcctcagtt ccatcccgtt
     6481 tccggtgggg gtgtaataca cgcggccttc cgggaggtag gcctggggta cgtaatgttc
     6541 cggatagtcg tgggaataaa ggtattccag cctggcggca tccgcgcctc cggcggcagc
     6601 cagcttcttg cgcgttggcg tccgcaggtg ttccggaact gccagcgttt tcccattgcg
     6661 cacgtcatcc atggcggcat tgatgcccac gtaggcggaa ttgctcttgg gggccgtcgc
     6721 caggtaaacc gcagcatgcg ccagaggaat gcgtgcctcc ggcatgccga ccatttccgc
     6781 cgcgcgcgcc gcgtccaaag ctacgcgcag ggcgttggaa tccgccagcc ctacatcctc
     6841 cgacgcggcg atgaccagcc ttcttcctat aaagcggata tcctccccgg cttccagcat
     6901 catccccagc caatacaggg cggcgtccgg gtcagagccg cgcatggatt tgatgaaagc
     6961 ggaaatcgta tcgtaatggg aatcccccag gcgatcgtac ctgatagcct tgcgctggat
     7021 ggattcctcc gctacggaga tgtccacatg aatgacgccg tctttccccg caggagtgga
     7081 cagaacagcc acttccagag ccgtgagcgc cttcctggcg tctccgtccg ccttggcggc
     7141 caaatggttc agggcttccg cctccatatc cacgcgggat gcccccagcc cgccagcacc
     7201 agcaaaaaga gccatgactg cgagccgtcc gcatt
//
LOCUS       DAWEMX010000124         7219 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4208_length_7219_cov_12.731435, whole genome shotgun sequence.
ACCESSION   DAWEMX010000124 DAWEMX010000000
VERSION     DAWEMX010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_7219_cov_12.731435"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1288 a         1740 c         2463 g         1728 t
ORIGIN      
        1 tatgccttgg caagtccaca tgcttatgat gaaggaattt cagtggaaac cagatgaatg
       61 accctatgca ggaggatggg gacggttatt ccggttttac atgaagtgag ggaaatggga
      121 aaaggattaa ttattggcga ttttagtgtt ttttaaatga tccggatttt ttgcgcgggc
      181 tatggggaat gaaggtttaa gtgcctgcgg atttgtaacc ggatgtcaaa caacgatatt
      241 gaacagcaag gggaaatgat aagaaggtgt tctcaaaagt ttttctgcgt atggagcatt
      301 tcatctttcc ctttatagaa aaatattttc ctcccgcttt aaaaagaaaa ccggggcagt
      361 tttgtttgat ttcataggag tatgacatga aatgaatgag ttttttttct ctttgggaat
      421 ctaatatcag tattgattat gtgtaaagaa tgtggatgcg ggggggagct ctcccatgaa
      481 agtcacggcc acggcatgga cgtccatgtt ccggtgttgg atgccaatga tcggctggca
      541 gagcgcaaca gaggtttttt tgctgcaaaa aaccttttgg ttatcaatgt tttttcttct
      601 ccggggtcag gaaagacttc cctgttgcag aagacggcgg agatgctccg cggccgtgtg
      661 cgcatggggg ttatcgtagg ggatttggcg acggataatg atgcggaacg cctgagccga
      721 gcggatatcc ccgtggtgca gattacaacg ggcaccatgt gccatctgga cgcccgcatg
      781 attgcggagg ccatgaagaa aatgcctctg gacgatctcg acgttttgat tattgagaat
      841 gtgggcaatc tggtttgccc ggcttcctat gacctggggg aaggcgtgcg cgtggtgctg
      901 ctttccgtga cggagggtga ggacaagccg ttgaaatatc ctcccatgtt ccattctgcg
      961 gatgttgccc tggtgaccaa gtccgatctg gcggatgccg tggattttaa ccgtgacgcc
     1021 gccctggctg cactgaacaa ggtggctcat catgcgcatg tgattgaggt ttcttccaag
     1081 accggcgaag gaatggaggt ctggtgcgag gagattgtgg aacgtgcgcg ccgcgcccgg
     1141 gaaggcacga ttcagcatca tggccatcac caccattgaa caggccgccg cggcattgaa
     1201 atttgagata gccggcatgg ttcagggggt cggcttccgt ccccatgtgt accgtttggc
     1261 ggtacggcac ggattgaagg ggtttgtgcg caatacggag tccggcgtgg agattcatgt
     1321 ggaaggggag cccggcgctc cggagcgttt ctggaccgcg ctgatggacg gtctgccgga
     1381 gcatgcccgg gtttatggag tggagcggac cgtatgcgag cctgccggtt ttgaggaatt
     1441 ccgcattgag gaaagcgatt ccacccccgg aggcgttccg gtgatgctgc cggatctggc
     1501 tccgtgcccg gagtgcctgg aagaaatgca cgacccgttt tcccgccggt atcattatcc
     1561 gtttaccaac tgcacgcact gcggtccccg ctattccatt attgagacaa tgccgtatga
     1621 ccgagcgggc accagcatga aggggttccg aatgtgtccg gagtgccgga gggagtacca
     1681 ggatgtggag gacaggcgtt ttcacgcaca gcctatcggc tgcccgtcct gcggcccttc
     1741 cgtgaaggtg ctgttttcag atggttcaga actggggttc ggccatgggt ttgatacccc
     1801 cgcggcacag gtggcatggg tgctggcgga cggtttgatt gtggccctgc tgggcgtagg
     1861 aggttttcaa ttgctggcgg atgcttcttc ggaagctgcc gtcaggcgtc tccggcggtt
     1921 gaaggagcgt gacgccaagc cttttgccgt gatggtgccg gatgtggctg cggcggagag
     1981 gctgtgccgc ctgtcggagg aggaaaggcg cctgctggct tctccggccg cccccattgt
     2041 gctggcccag gggaggaagg atgtggatct ggcgccttcc gtgtgcatgt tcagccgttt
     2101 tgtggggata atgcttccgt cttcccctct tcatgcgctg ctgatggatg tgtggggaaa
     2161 gcctctggtg gtgaccagcg ggaacctgag cggcgagccc ctgtgcgtct ccgtggagga
     2221 ggggctggag aaactgggcc atgtggcgga tgtgtttttg gtgcatgacc gtccggtagt
     2281 gcggcccgtg gacgattccg tggtccgcgt tacggacggc agggccatga tggttcgccg
     2341 cgcccgcgga tatgccccgc ggcctgtgtg gcgcacatct gcggcggctc ccgacgtgct
     2401 cgccctgggg gccggactga agaataccat ctgctggctg aaaaggggtg tggctgtcat
     2461 gagccagcat ctcggtgacc tgagcagtgc ggcttccctg aacgcttttg agcggacggt
     2521 ggaggcgctg ggcagaaccc tggatgccgt tccgcaggta attgccgtgg acgcccatcc
     2581 ggacggcccc acttttgccc ttggccggcg tctggcgcgg aagtggaatg caacggttgt
     2641 ccctgtgcag catcatcagg ctcatgtgct ggcctgcgcc gtggaaaatg aaacgccgtt
     2701 tcccgctttg ggcatagctt gggacgggac ggggttcggt acggacggaa cggtatgggg
     2761 aggggaattt tttgtgttgg aggaagaggg tgagccgcgc agggtggccc gcctcaggcc
     2821 gtttcttctt cccggtgggg acgaagcggt tcgggaacct tcccggtgcg cctgttccct
     2881 ggcccgtcag atggggccgt ggcgtacgta cgggcttgat aagcgtctgg agagggggat
     2941 gcctgtgcgg aaacgcgagg ttctggaggc gatgatggcc cggaatatcc attgtccgtc
     3001 ctcctcgtcc atggggcggt tgtttgatgc cgtggccttc tggtgcggtt ttgacggtgc
     3061 ggcggggtgt gagggccatg ccgccatgat gctggaatca tgggccgcgg aagcttccgg
     3121 ggaaaaaatg ccgggggacg cgtatgaatg ggtcatgcat gaagaagagg gattgctgga
     3181 actggattgg cggccgctga tgaagtctgt ggatgaagat ttgttggaag gcgtttcccg
     3241 cggctgcatc gcccgcaagt ttcatgagag cctggtgaat ctggtttttg acgtggcgga
     3301 gcgtttctgc ctggataggc tggtgctggg cggcggatgt tttcagaatg cctttttgct
     3361 tgaaggactg gcggggatgg cccagtctag aaggtgccag ttggctctgc cccagcgggt
     3421 gccgtgcaat gacgggggaa tttccctggg gcaggttgcg gcggtcgtac gccaatggaa
     3481 aggatagaat atgtgtttgg ccgttcccgg aaagattgtg agcgtcaatg agacggaccc
     3541 tttgttcaga ttgggggtgg tggattttgg cggcgtgacc cgtgaagtga acctagcctg
     3601 tgtgccggaa gccgtgccgg gggattacgt catcgttcac gtgggcatgg ccctgagcgt
     3661 gctggacgag gagaccgctc ttcaaacccg cagggaaatg agagaaattg tggaaaatac
     3721 ggatccgctg tagggagggg cgttttccga attcaattaa tgatttatgc aggaggaagt
     3781 ccggcagctg attgaagaat tgcgccacgc tgtgacgcgt ccgtgggcgg taatggaggt
     3841 atgcggaggc cagacgcatg cgattgcttc tctggggctg gaggagttgc tgcctcccgg
     3901 cctgcgcctg attcatggcc ccggctgtcc ggtctgcgtt acggcggtgg agttgattga
     3961 ccaggccgtg gaattaagcc tgaggcccgg cgtggttttg tgcagctacg gggatatgat
     4021 gcgggtgccg ggctcccgcg gggatttgtt ttccgccaag gccgggggag ggaacgtgct
     4081 tttgatgtat tctccgctgg aagcggtcgc ttatgccgga agccatccgg atacggaagt
     4141 ggtgtttttc gccgtaggtt ttgagacgac ggccccggct acggctcttg ccctgcagca
     4201 ggcccgtgca ctggggtatg ccaatttttc cgttctctgc gcccatgtgc tggtcccccc
     4261 ggctctggaa tggctgatgg accaggatga gggaaggccg gacgcttttt tggctcccgg
     4321 ccatgtctgc gccgtcacgg gggaaatgga ttacgggcgt ttggcctccc gttaccggac
     4381 gcccatggtg gtgacggggt ttgaggctcc ggacctgctc cgcggcattc tgatgtgcgt
     4441 ccgccagctg gaagccgggg aatatgcagt gcggaatgcg tacggacgtt atgtgaagcc
     4501 cggtgggaac agggcggcac aggaaagaat gaatgaagtt tttgagccgg aagaccgcca
     4561 ttggcgcggt ctggggctga ttcccggcgg cggcatgagg ctgcgccggg agtggaatga
     4621 catggatgcc gttctgcgtt ttgaatgcgg aaaggggaag ccgggagcgg ataaagcttc
     4681 cggatgcctg gccgggaagg tgctgagggg gctgattcgt cctggagagt gccctttttt
     4741 cggatcgtcc tgcacgccgc tggccccgct gggggctccc atggtgtccg gggaaggggc
     4801 ttgcgccgct tattatcatt ataaaagaag ttgatgccat gtttgagtgt cctgttccgg
     4861 agcctgtttc cgaccgtatc cagatggctc atggcggcgg aggccgcttg atgaatgagc
     4921 tgatccgttc cgtgtttctg agcgccttcg ggtgttcttc tggaggcgtg cagaatgacg
     4981 ccgccgtatt ggagattcct ccggggaggc tggcgatgac gacggacagc tttgtggtgc
     5041 agcctttgga gtttcccggc ggctccatcg gttcactggc ggtgcacgga acggtgaatg
     5101 atctcgccat gagcggcgcg gagccgttgt atttgacggc gggctttatt ctggaagaag
     5161 ggcttccgct ggaagtcctg gcccgcgtgg cacgggatat ggctgccgcg gcccgtgcgg
     5221 cgggcgtccg tattgtgacg ggggatacaa aagtggtgga gcgcggaaag ggggacggca
     5281 tttacattaa tactgccggg gttggcattg tgcgccatgg gttggagatc agcccttctt
     5341 ccgttcgtcc gggggattcc gtgctgctca gcggggattt ggggaggcac ggcatgacga
     5401 ttatgagcct gcgcgccggg ctgtctttcg gagacggcct ggaaagtgat tccgctccgt
     5461 tgcatgagtc cgtggccgcc gtcattcgtg ccggcattcc cgtgcattgc ctgcgtgacg
     5521 tgacccgcgg cggattgacc gccactcttt cggagattgc ggaatctgcc ggcctgacag
     5581 tgaagctgaa tgaaatgtcc attcccgtgc gtgaggatgt cagggcagcg tgcgggctgt
     5641 tggggctgga ccctcttcaa gtggcctgtg agggacgtta tctggctgtt cttccacggg
     5701 agcatgagga agaggccctg aacctgatgc gcggctgcgg cgtatctgcc ggagcctgcg
     5761 tcataggccg tgtggaggaa ttggggacgg ctcccctgct gatgacggga cttcttggag
     5821 tggagcgggt gttgacgatg ccttcaggaa tgcagcttcc ccgcatctgc tgattcctgg
     5881 tgctgcatga ttggcgtttt gtggcaggga agcggtggaa cccgcttccc tgccggtccg
     5941 gtgtttcata ggggtgccgg agcggctctg tgcgcttccg ggggaaagcg cagccggctt
     6001 ggtatgggga actggttgtt gggagatggt tcaggaggga gtgtctttac ctgtcccggc
     6061 atcgggcaac gcttttaatg ccgctttggc ctgtgcatgg tcctgggagg ctgccttttc
     6121 cagccattcc cgggcagttt ccaggttttt ttccacgccg aatccgtaca ggcagatgat
     6181 tcccatgttg tattgctcgg aggcgtagcc ggctctggcg gcgcgaagat tcctgttttg
     6241 ccattccctg gcttccgctt ccgtgaagtc ctggctcttg cagaattggt cccacgtttt
     6301 tccgaatttc atggcagccc tcgcaaatcc cgacagggcg gattttctgg tgtattcatc
     6361 cgccaggcgc atgttttttt ccactccgtc gggtcccagc ctgtacaggt gggccagtcc
     6421 ccagtatccg ttggggctgc ccagatccgc cgctttttgg aagagctgca gcgcgtccgg
     6481 gtatttttta tgggacatgg cgaataccgc attggccagg aagcgttccg ctttgatgaa
     6541 ttcgtaaaat tcgggaatag ccgcccattc ggatttaagt ttgttcctgt ctttcctgga
     6601 tttgggaggc atgccggaag gcggggatat tcggaaaacg ggaatgtagc cggaagcgcc
     6661 catattggcc agcccggcca tcagaaagca gcccggctgg aggcgccaca tgtgcatgta
     6721 caaaacgcgc tctgctggca gagattcctt ctcaatccag tccggcaggt tcatgggggg
     6781 gccgtaaagt cccgtcaggc cgttcagcat gcgcttgtac agggatttca ggcgcgtatc
     6841 gtattcctct ttacccgctt tggtggtgga gtaaaaggaa acggctttca gacctccatc
     6901 ctttccgtac tcgaaatgca gggagaagaa aaggcctcca ataggttttt tcgtcttgta
     6961 ggctccgttc agccctgtac ggctcaggta ggcgtctgtt tccggccctt ccagggattt
     7021 gcaggttttc agggaattca gaagctggga ttcctgcaaa cctgtttcca agggtccgta
     7081 aagaggcgag gcagcgccgc aggaagctgt ataaagcaca gccatgtagc gatagcaatt
     7141 ggtcaatttc atgggataga tagggcaggg atggaagtgg tgcttcaata gcggattagg
     7201 aatccgaatg aagtgttca
//
LOCUS       DAWEMX010000125         7118 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4263_length_7118_cov_11.156874, whole genome shotgun sequence.
ACCESSION   DAWEMX010000125 DAWEMX010000000
VERSION     DAWEMX010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4263_length_7118_cov_11.156874"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4253..4262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1453 a         1825 c         2264 g         1566 t
ORIGIN      
        1 tttggagggg gctgcgtcct cctgcccgat tttcaaatcg ttccaggcct gttccgcgct
       61 ggccatcggg cagcggtgcg tttctttggc gccgggcaaa gaatagatga tgccaataag
      121 ctggccgagg aagaggccgg cgatgatgac accgatagcg acggagaaag gcctgctgaa
      181 gggggatgtt tcctgcttca cattcagcag catgatgatg aacgcgaaga gcaccataat
      241 ggccccggcg tatacaagga tttggagaat gcccaggaag tgggctccca gcccgatcat
      301 gacggcggcg gtggctccaa aggaaagagc catcatcatg gcggaggaga cggggttgcg
      361 catgaagacg accatcaggg acaggaccac ggccagggcc ccgaaaacgt aaaaaagtat
      421 gtcgctggca aagatgttca taacgtacgg gaaatggagt gttcgggggt tatttgtatt
      481 ggttccactt gttgaccagc cccttgcggg tgccgcccag ttcgtagagc ttggccttgt
      541 tgtggatggc ctgggtttta tccgtggggg tgaacaggta gttatggctc atgaagatgg
      601 cctgttccgg gcaggcttcc tcgcacatgc cgcagtagat gcagcgcagc atgtcaatct
      661 gaaattccct gggggctttt tccactttgc cccacggtcc ttcctgtatg gggccggggg
      721 tgatggtgat ggcgcgggcc gggcagatga attcgcagag ctggcaggaa acgcagcgtt
      781 cacggccgtc ttcgcctttc accagcacgg gagccccgcg gtaatatttc ggcagatgtt
      841 tgtcccatct ggcttccgga tattgcatcg taacgccgat gccggaaccg gccagttctt
      901 ttttgttgcg ggatttgccc agcagagcca gaacgaggtg cctgatagtg agccaaagac
      961 cgccaagaat ggattgcaga tagaaccgct ctccaaggga aatcttcgga cgcttgatcg
     1021 ttttgaaggc cataatagta atgttgtcgg aatgaagaag gggggattac ttgacgaagt
     1081 acaggatggc ggctgtaagg aagatgttga tgacggccag ttcaaagaag accatccagc
     1141 cgagcttcat cacctggtca tagcggaagc gggggagcgt ccaccgcacc cagacgaaga
     1201 agaaggcgaa gaagagaagc ttgaacacat atgcgatgag ctggcagatg accgccaccc
     1261 agttggctac gttttcattg agccagccgt ccaggccgaa gccgatggac cagcccccca
     1321 ggaacagcgt gatgacaagg gaggatccca ccaccatggc ggcatattcc cccatgaaga
     1381 attgggcgaa tttcatggag gagtattccg tatggtagcc gcccacgaga tccgtttcac
     1441 attcggacat gtcaaacggc gtacggttgg cttccgcaaa aatggaagtg aggaatatga
     1501 tgaaactgat agctacgggc accagcagta tccagcgctg ccagctcagg ccctcgcccc
     1561 acacgggaag caggagccag ccattggcgg cctggtattc cacgatgttg gacaggtcca
     1621 gcgtgctgta aatcatcagc acagggatga tggacagacc catgctgatt tcataggaaa
     1681 tcatctgggc tgaggagcgc acgccgccca ggaaggggaa tttggagttg gatgaccatc
     1741 cggccagcac cagtccgtac acggagaggg aggagatggc gaacatccag agggggccta
     1801 cgctgagatt ggccaccgcc atgttgacgt ttccgtaaga ggtttgaagg tctccggcaa
     1861 agggaaccac agccgccgtc atcagcggtg gcgcaagcac caggatggga gcgatccaga
     1921 agtatacgcg gcgtacgaag gggggcgtga aatcctgttt caggaacagt ttgccgccgt
     1981 ccaccatggg ctgcacgaga ccgaagatgg ggatgtcgcg gtccaggcgg aaaaaacggc
     2041 acaggttggc gattttgccg tcatggggca acccgaagta gtccagcgtc tttttgaacg
     2101 gcaggtcgga tttcataccg aagcggcgga agatgcttaa cggaatgccg gcgcggttgg
     2161 ggcctacgcg gtcctggatc caggcggcca cccggcgttc aatgtacacg cagaccggaa
     2221 tgaacagaat ggtgatggca aagcagatga cgattttgat cagcagagtg atgatataaa
     2281 accacacagg gttgctgagc acctcgtcgc aaaaggttag aatcgaatca atcatggcgg
     2341 attaacaggt tgtatgcgtg tgaagtgtga ttagcggccc tggttttttt cgcgttccac
     2401 cacgggaatg gtgacgccgg tttccagaat gggctggccc ccgtctccga tggaacccca
     2461 ggagactcca ttgagtgcgc cgtattccgt gctcatggcg gtgaggatgt ccagggcgga
     2521 gttgaagtct ttcagagcag gattgcctcc cagcagaagg gtgatgtctc tgaaaatctg
     2581 ccagtcgtcc cgggcgtatc ctatgggctg gatagcgcgg ttcaggcgtt gaatacggcc
     2641 cgtgacatta atcatggtgc cgaatttttc agcgtaggtg acgcccggca gaaccaggtc
     2701 cgcatggcgc gccgtttcat tggcgctgtg ggcggtcatg aacaggtagt ccagcttgtc
     2761 cagatcttcc gccgtgaagc ctgcttccgg ggagatgagg tcttccccca gggagaggat
     2821 gcccttgatg gaaccgctgc gaactccttc gcgaatggcg tccagcctgg aaccgggttc
     2881 gatatccagc accagcctgg ccccgttcgt gttcgggttg cgatccgcag aaataagcat
     2941 gccgtcgctt tctcccatgc ggggaacgat gtccaccata tccgtgccga tttgggcggc
     3001 caaatggcgg accatgtaaa gttcttcatt ggtcatgcgc ccggaggcaa tgatggcgag
     3061 ctggttcgga gcaatgcgct tgacggcttc cgcggcggaa gccagagccg gttcccaggt
     3121 ggaggggcgg tggggcgctt ccgcatccgt acggatgacg ggctggggaa tgcgggtttc
     3181 cgcattgata tatttatagt taaggcggtg agagtccggc atccagcagg agtttacggc
     3241 atcattctgg cgcggggtaa tgcggtatac cttgtcttcc cttgtccaga gggtgatatt
     3301 agtgcccgtt ccgcagtcca cgtcaatggt ctttgtttcc ttcaggaacc agacgcgcat
     3361 tttaaagcgg aaatccttgg aagtaagggc tcctaccggg cagaggtcca cggtgttcat
     3421 ggagtagttg ctgtccagct cgcggccggg gtaaaccgtg atggcgttga aggtgccgcg
     3481 ttgggacatg gtgagcacct catcgtgcat gatgtcgcgc atgaagcgca cgcagcggcc
     3541 gcagagcacg cagcgttcct ggtccaggtt cacgcgcggg ccaatgctca ggttttttcc
     3601 ttttttggtc tttttctctt caaaacggcc ttccacctgg ccgtagtcgt tggagtattc
     3661 ctgaagcttg cattccccgg cctggtcgca gatggggcag tccagagggt ggctggtgag
     3721 cagaaattcc attacgccgc ggcgcgcttc actggccagg gggctgtccg tgcggatgcc
     3781 catgttttcc gccaccgtgt ttgcgcagga gatgatgggg cggggcatcc actggatgcg
     3841 ctgatagccg ttttcatcat aggagggtgt ctgtcccgga gcgagcctgg gcggcatccc
     3901 ctgttccacc atgcacatgc ggcaggagcc ggcgacagcc agcttggggt ggtagcagaa
     3961 gcacgtaatg ggcacgccca cggaacggca ggcgtctgca atccgcgtgc cgcgcgggaa
     4021 ccggtgccac gttccgttga tttggacgtt caccttgcct tccgcggcgg caatgtcttt
     4081 ggggagtttg gaagcttctt cactcatgat gacggatggg agagggatgg ttttgagaac
     4141 tggtgttgcg tgctgctgtt tagcgggtga ggaaacggtt ttgctccctg ccttcctccg
     4201 tgtggggcag acaggcgttg aatggatccg tcagggctac gaattcttcc ttnnnnnnnn
     4261 nngggtgggc caggaggcgg cttcaccaaa ggcgcagacc gtttttcctt caatctggtt
     4321 ggccacttca atgagcagat ccacatccga cggggcggcc tttccttcca ggatgcgggt
     4381 gctgagtttc atcatccacg gacagccttc ccggcaggga gtgcactgcc cgcaggattc
     4441 atgggcgtag aaacggttga tgttgttcat ggcccagggc atggagcggg agtcgtccat
     4501 gacgatgacc gcgccggaac cggacatgga gccgtgccgg gcgatgctgt ccagatccag
     4561 cggaatgtcg aagaaggtga tttttcccat ggtgccgtcc ggattctgca ctttgtattc
     4621 cgcatccgtg cgcatgactt tggaggagga gcctccgggt atcacggcct tgaactggcg
     4681 tccgtcccgc gggccgccgc agacgtcata cagcagttca ccgaaggtca tggagccgga
     4741 gacgatttca tagaacccag gacgtttgac gtcgccggaa accccgatga tgcgggtgcc
     4801 gggggaacgt ttggctcctt cctggctgta ggcttctcct cccatggcca tgatgtgttt
     4861 cacctggcag agggattcca cgttgttgac aatggtgggg catccgtaca ggccgatggc
     4921 ggccgggaag taggggggct tgatgcgggg ataggggcga tccccttcaa gggagttgag
     4981 cagggccgtt tcctcaccgc aaatgtacgc tcccgctccg cggtgcaggt ggatttccag
     5041 attatagccg gacccctgga tattgctgcc caggaagccg cgttcgcgag cttcctgaag
     5101 ggctttgagc atgatttccg cggcgatggg aaattcttcg cgcatgtaga tataagcctg
     5161 atgtgccccc actgcgtagc tggagatgac cattccttca atgagctggt ggggatcctg
     5221 atggacaatg tagcggtcct tgaacgtgcc tggttcggat tcgtcacagt tgcaaaccag
     5281 gtaaacaggc ttcgtgttgt tcggcggaat gaaggtccat ttcacgccgg tggggaaccc
     5341 cgcgccgcca cggccgcgaa gaccggattt tttcacctcg tcaatgacgg ccctgggctc
     5401 catgccgagc gcctttttca gttcttcata accgccgtcg gccagatagc agtcaatgga
     5461 ggtattccag ccctcgcggt tgacgttttt cagaatgcgg cgcgtttccc tggggtgggg
     5521 ttctttaccg ggaagatagg tgatgctcat ggcgaaaatc tggtgcgtgg gaaatggaaa
     5581 gggttaggct gtctgatatt tggcaatcac ttcttccagc acttccggag tgactttttc
     5641 gtacaggaga tcgttgacca tcatgttggg gccaaagccg cagttggcca ggcattccac
     5701 tccttccacg ctccacaggc cgtccgggct gactccaatg gggtggtggt ggtcgatgcc
     5761 gtttttgtca atgcccagac gcgtgcagat ggcgtcgaac aggctgtccg cccccgccat
     5821 ggcgcaggaa agagtgcggc atacgcggat gtggtttttt ccagggcaca tctggcgcag
     5881 accgggatag aaagtcacca cgcccagaac atgggcggcg gagatattca gtttttctcc
     5941 cacccaggca atggcgtcgc cgctgatgaa gccgaatttt ttctgtattt catgcaggat
     6001 gggcagcacg gcggattttt gcttgccttc cgggtaatgc gtaacgtatt cgctggcggc
     6061 ggcgtcaagt tccggcgtta cttcaaaggc cgggtaaaac cgttcacccg ggctgggctg
     6121 gtgggcgata gtcgcgtcga tggcgttttc agcgtaatcg gacatgggga gaaagtttgg
     6181 tgtttgcgtt gaagtggatg aaagtggcgg gtgtttaacg gtcgcattcg cccatcacga
     6241 agtcgaagga gccgagaatg gccggaacgt cggaaaccag gtggcctttc atcagttccg
     6301 gcaggatgga caggttgcag aaggaggggg agcgcatgcg caggcggttg ggaagcccgc
     6361 cccccttgga gtccaggaag aaacccagct ctcccttcgg gttttccgcg gcaaagtaaa
     6421 cctgcccggc aggggcgttc acccccatgg tcgtcgtcat gaactggcga atcagggatt
     6481 ccatgtccgt caggactttt ttcttttcag gcagcgtgcc tttggtgtcc accatgttga
     6541 tggggccgtc cggcatggtt tcaatgactt ggcggatgat gcgcagggac tggcgcattt
     6601 cttcaatgcg cacggtaaaa cggtcgtagc agtctccgtg ttcgcctacg gggacgtcga
     6661 attcgtaatt ttcatatccc agataggggg tgagtttgcg caggtcccgc ttaatgccgc
     6721 tggctcgcag gttggccccc gtaatacccc aggaaagcgc tttttcacgg ctgatgacgc
     6781 ctacgccctg aagacggtca atgaagattt tgtttttgag caggagggct tccgtttcat
     6841 caagggtttt ggccgcttca tcgcagaaga cgagcacttc cttgagcatt tcattgggaa
     6901 catcacgtgt ctgtccgccg atgcgggtat aggaggaagt gaagcgcgcc ccagtgagct
     6961 gttcgtaaaa attatggatt ttttcacgtt cctcataaca gtacaggaaa acggtcatgg
     7021 cgcctacgtc catgccgtac acgcctacgc ccaggatgtg ggaggaaaag cgggaaagtt
     7081 ccagggccag tacgcgcagg gcctgtccgc ggggcggt
//
LOCUS       DAWEMX010000126         7054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4318_length_7054_cov_13.536077, whole genome shotgun sequence.
ACCESSION   DAWEMX010000126 DAWEMX010000000
VERSION     DAWEMX010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4318_length_7054_cov_13.536077"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5801..5810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1591 a         2141 c         1863 g         1449 t
ORIGIN      
        1 gcccccagcc cgccagcacc agcaaaaaga gccatgactg cgagccgtcc gcattattgc
       61 tcggcatgac aaggccaaag aagcggtaca caagcaaaaa gaagacgaag atggcaaaag
      121 gtatccatcc ccagcgggct tcctccttcc ggcgggccat ggccaattca aaacagggat
      181 ctttaaacgc aggcaattgc ttggcagaag aatctctgaa gaaatacata tcgcccgaag
      241 tctctattat tacgcctttc ttccgtcaat aacattcaat gccagcccgg attttttccg
      301 cagaaaattt tcccctctaa caaatacgga atatcggttt ccgcccatta ccttattgtc
      361 aaaccaaagg gctttgaaaa ttcatgaaac actcttgatt ataaataatt ttcccctttt
      421 tcggatactt gctcttcgtc ttttcccatg ataaaaaagt ctttttgcat cattcgaatt
      481 ttaacacccg ttcaatttgt catacacagc cccagtttcc cataaaaaag actgcctccg
      541 ctgcaggagc ggaggcagtc cgggggtaat ataaaaccgg aaattacaga aggttacctg
      601 cccagaatgc ccttgaagta ttccagcgtc gtcgccaggc cttccggcaa agtatgcctg
      661 ggctgccatc cggcattgcg gagcttgtcc gcacaggcgc gggaatgctt gacgtctccc
      721 ggacgttccg gggcatgaag caccttggag gaagacccgg cagccttgat aatgttctgc
      781 gccagctctt caatggtgat ctggccgccg taaccggcat tgaacacgcc ggtgacttcc
      841 gggtgttccg ccacaaaggt gagggctcct acaatgtctt tcacataaat gaaatcgcgc
      901 gtctgggagc cgtccccata cacggtgatg tcttctcctt tgacagcttt ttcaatgaaa
      961 atgggcacgg ctgcggcata ggcgcccttg gggtcctgcc gggggccgaa gacattgaag
     1021 aagcgcacgg ctgccgtatt gatttttcct tccgcccgga acatgttgag gtagtattcc
     1081 ccatccagct tggtaatggc atagggactc ttgggttccg ggtacatggt ttccagtttg
     1141 ggcaccgtgg gattgtctcc gtaaatggcg gcggaagacg ccagcacgat ttttttgact
     1201 ccggcggcgg aagcttcctc cagcacgttc agcaaaccgt tgacgttgat gtcgatgcat
     1261 tcgctgatct tgctcatgga ttccggcacg gagacgagcg cggccatgtg gaaaatatag
     1321 tccaccccct gcaccgcctg gcgcaccagc tcccggtcgc agatggaacc ttcgatgaac
     1381 gtgtgcctga gcccttccaa gttcctgaga tagcccgtgc gcaggttgtc cagcacacgg
     1441 atttcctccg ccttatcctg atagtgttcc acaatgtggg aaccgatgaa tccggcgccg
     1501 ccggttacga gaatcttcat gtttttccga tgttggtaga tattggttaa tagcggtcca
     1561 caatggcctt catttcatcc atctgcgcct caatggattc taccagggcc atctgcgtgc
     1621 ggcagcggtc caggttgtgt tccggacggt ggattttaaa atacacgtca ccggacaggt
     1681 agtccgtcag gaagcggatg ccgcattcca tcgtgagcaa tttgccggag aacggcagca
     1741 ggctcttttc cagaggcgtc aggaaactct tggcggaact caggtaaccc tggaccagag
     1801 cttcaaacat gaacatgcgc atcgtgacct tggagacgtc cttttcatct tccgcggcgg
     1861 gagacgtggc cgtccggatc atgtcgccga agtcgtaaat ggcggaaccg ggcatcgtgg
     1921 tatccaggtc aatcacgcaa atgccttctc cggttacatc gtccagcatc acattgttca
     1981 gcttggtatc attgtgcgta cagcgcaggg gcaattcccc ggatttcagg taatccacca
     2041 ccagatggca gtccgactca cgggaaaggt accagtctat ttccttctgc acttccttca
     2101 cgcgccccag ggggtcggac tggatggcct ctttcagagc cgccaggcgg gaagtggtat
     2161 tgtggaaatt gggaatggtt tcaaacaggg gctctccggg caggtctgcc gtaagcttct
     2221 ggaattcgcc gaaggcgcgc gccgcttcct ggcactgctt ggtagtttca atctggtcat
     2281 acgtgcgtgc gcgttcaatg aacgggtaca cgcgccagca gttgccgtcc gaatccaggg
     2341 cgtacggctt gccgtccacg gagggaacca gcgtgagagt gcggcggtaa gcttccgggc
     2401 actgttcctc cttcaggcgc ttcagggcgt ggtccgtcac gcgctttacg ttctccatca
     2461 gagggatggg ctgcttgaac acgttatggt tcacgcgctg gaggatgtag cggatgcgct
     2521 gtcccgcctg atccacccag atgcagtagg tgtcattgat gtggccgctg ccgtaggaat
     2581 aaccgtggac aaaatcgccg cggagatcga acagggttga tacagccttg agatcgtgca
     2641 tagggaaaaa ggcttagttg agcttttcag ggtatacgcc ctgctggata agtttgttga
     2701 tgccttccac aaccatgggc ttgtcccccg gataggtcat gccgaaccag gaggaagaag
     2761 tgggaagaac ggcgcaatcc gcccggtccg catgaatcag ggcgtccacc acggtgggaa
     2821 taaagcattc gctcttgggt tcctggccgt gttctttcag gaagtcaatg aaatgatcct
     2881 ccgtcagttc gatgaaggag ggggagaaca gccagaaatt catggaaacc agttctgcgg
     2941 gatccaccgg caggcgttcg cccttcaggt tgttgccgcg cactacgccg tcttcgtcag
     3001 cctggatttt cacgaattcc tccacgccat ccagaaggcc gcccttgatg gagcaaatgc
     3061 cgcggttcac gtccccgttg tcggagaggg tgttcttcag cggatagccg atcatgccgt
     3121 aatgggcctt gccgggttca tccgtagtgg aggtcagaaa cttggcggcc tgcacaaaag
     3181 cgtccgcacc gtaaaagtca tccgcattca ctacggcaaa gggttccttc accacgtggc
     3241 gggcggcacg cagggcgtgg gcggtgcccc agggcttttc ccggccttcc ggcacggaga
     3301 agccttccgg caggtcgtcc agggactgga aagcgtaatc cacctcaatc ttgccggcaa
     3361 aacggctgcc gaccttttcc ttgaaaatat cttcaaagtc acggcggata atgaatacca
     3421 cttttccgaa accggcccgg atggcatcat aaacagagta gtcaagaact acttccccgt
     3481 tggggcccat cgggtcaagt tgcttgagac cgccgtaacg gctgcccatg ccagctgcta
     3541 aaacaagaag tgtaggcttc atcatacgca gaccattatg accaagcctc cccgtttggc
     3601 aacaggaaaa tgccggaaaa ccggagaaat ccgcccgtta aaaaacgtaa gagcaccaaa
     3661 gccccagcac tacttttttg aaatcgtccc gcccgtagcc gcaataccac cccaggaaag
     3721 gctccacccg cagaccgccg gaaacggccc acagcgcccc cgtgcgcacc atcagctctt
     3781 tcaagccgtt cctccccaga taatcgtggg caaaacccag ggaaaccgta ttcaccatgg
     3841 cgacggattc gcacagcagg ggctgccagg taacgcccca ctgggaatat gcaccctcct
     3901 gtccggaatc atacaggaag gaaccgcgga aagaccagga aggggtggga ttccatttaa
     3961 gggacaggcc cggctccgcc ccgttcttca ggggacagga atcgtaccac tgcccgttga
     4021 caaaaagccc ggccgtgcat tcgtccgcca gataatactg gacttccccg tgtaaagtgg
     4081 cctgactgaa atggcggccc tgccagttgc ggatggcgaa aagttccccg ctcaccgccc
     4141 aggaatcaga cagggaggct ccgccggaaa tctggccctc cgtggaatca gcccccatct
     4201 tttccccccg gtggacatag tacgaacggt agccgccaag catctccccg cccatgcgga
     4261 tgggggaacg gccgtccacc tccgtcaggg gcggcggccc gtcatacccc aggacggcct
     4321 gcacggcaca gcacaggatg atggaaaaca gcctcatgaa agacttttaa cataattcgc
     4381 tgcgccgctc caacgccaaa acggtgcatg cacattctcc gggcttggca aaacgggaat
     4441 ctgcccttac cctcccgcgc atggttcatt tcaccctgtt cgggatacct gtttacatcc
     4501 gccccagctt ctgggtggtg ctggccattt tcggcggcgc cctcagcatc agcagtgtgg
     4561 aagatctcat ttatccggcc ctgttcgtca ttgccggctt catcgccatc ctgtcccatg
     4621 aaatgggaca cgcgctggtg ggccgcaagc tgggcggcgg ccagcagacg attgttctgg
     4681 aactcttcgg cggcctgaca agcagccatg gcatgcaact gacgcgcgga gggcgggccc
     4741 tcatgattct ggcaggcccc atgatgaccc tgctgctggg catcatcagc ctgtggctca
     4801 cctggaacat cgtcgctcct gtcatgacct cttacaacct gaacttctgg gacctggcca
     4861 tcagcccctt cacagcagcg ctcatctccc ccaagctgta cattctctcc tgcctcatca
     4921 tgattggaga atggtggact atcctgaatc tgctgcccat ctaccccctg gacggcggcc
     4981 agctgatcgc ccagtacatc cgctccccca ggaaagtatt catgaccggc ttcatcacgg
     5041 ccatcctcat cggactgctc agtttccagc ttttccacgg ctactttatc cccatcttca
     5101 tggccctctt cgcctacagc aattaccggg aatacaaaaa cgcccccttt taatattatt
     5161 ccgagtttcc ttcgcgggcc ccgcccgttc tattcaacaa accatccaat atgtctcagc
     5221 aaaccgcaat cacccctacc cgcgcccagg atttccccga gtggtaccag caagtcatca
     5281 aggccgccga catggcggaa aattccgagg tccgcggctg catggtcatc aagccatggg
     5341 gctacgccat ttgggaactc attcaaaagg acctggacca gcgcttcaag gacaccggcc
     5401 atacgaacgc ctatttcccc ctcctgattc ctatctccta tctggaaaag gaagcggagc
     5461 atgccgaagg cttcgccacg gaatgcgccg tagtcaccca ccacaggctg gaagcgcaaa
     5521 aggatgaagc caccggcaag acacgcatga ttcccaccgg ggagcttacg gaacccttcg
     5581 tcatccgtcc cacctcggaa accgtcatcg gcgcggcttt cgcccgctgg acctccagtt
     5641 accgggacct gcccctcaaa gtcaaccagt ggtgcaacgt gatgcgctgg gaaatgaggc
     5701 cccgaatctt cctgcgcacg gcggaattcc tgtggcagga agggcatacg gcccatgaaa
     5761 cccgcgagga agccattgag gaaaccctca ccatgcacaa nnnnnnnnnn gacgtgctcg
     5821 ccatccccac cattccaggg gaaaagacgg aggcggaacg cttccccggc gcggaacaaa
     5881 cctacacggt ggaagccatg gtgcaggacc gcaaggccat ccaggcaggg acctcccact
     5941 tcctggggca gaatttctcc aagtcccaga acatctgctt cgccggaagg gacaacaccc
     6001 agcaattcgc atggacaagc tcctggggcg tttccacccg catgatcgga gcgctcatca
     6061 tgatgcactc agacgacgac ggactcgtct gcccgccccg cgtcgctccc cagcaaatcg
     6121 tcatcattcc cgtcacgccc aaggaagaaa gccgccaggc cattctggac cactgcgagg
     6181 aactggcgcg caccctccgc accaaaacct tccacggcca gccgctgcgc gtgctggtgg
     6241 acaggcgcga cctgggcggc ggcgccaaga aatgggaatg ggtgaaaaaa ggcgtgcccg
     6301 tgcgtctgga aataggcccc cgggacctgg aaaaaggctc cgtctgcctt cagcggcgcg
     6361 accggcccgc caatgaaaaa tccttcgtcc cggaaacgga actgatcgat accgctgcgg
     6421 atattctcca aagcatccag gacaccctgc ttcagcgggc cattgccttc cgggactccc
     6481 atatccgccc cgcctccact ctgcgggaat tggaagaaaa cttctccgga gagggagatg
     6541 cagactggct ccaggtgccg tgggacgggt ctccggaaga agaagaagaa ctggctaaac
     6601 ggctgcgcat ttccatccgc tgcatcccgc tcggcgagct gggccgcggc gagccggcac
     6661 cctgcatcct caccggacgc atgacgaaac gccgtgttct ctgggccaga agctactgaa
     6721 ccctgacacc ccctttcaac gggattgttc caagacaggc aaaacgggcc ttttcggaac
     6781 aatcccgttt tttacgcctc tccactttcc ccaaaaacgg catgaacaag aaacggagca
     6841 gcatacccca cagggcagaa tcctcccctc tcctgcccca tacgccggca acgacgcaca
     6901 catgtcggca aaacaggatt tttctctaaa aaaatcttgc caagacagaa aaatcttata
     6961 tactcttggc acacggacgc cataaggctc caatcgttcc aagcggatgt agctcagggg
     7021 tagagcgcaa ccttgccaag gttgatgtcg tggg
//
LOCUS       DAWEMX010000127         7054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4320_length_7054_cov_10.845549, whole genome shotgun sequence.
ACCESSION   DAWEMX010000127 DAWEMX010000000
VERSION     DAWEMX010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4320_length_7054_cov_10.845549"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1788 a         1837 c         1724 g         1705 t
ORIGIN      
        1 tagcattacg actatttgcg atgtaccgga attgcttgtc attgatggac aacagcgttt
       61 gacaacagtt tcattgctgg ttctcgccgt agctgaattt ttggagaagc atcccgattt
      121 agaaattgac actactccta agaaattaag aaattactac cttctcaata atgaagaaac
      181 aggggaaaag cgcttcaaat tgctactgac ccagggagac agaactgcct tttgttcctt
      241 gatggatgga acgccactgc cagaacacgt ccctggtaga atcgtagaaa attatgaatt
      301 tttctgtgga aaaataacac gagaaaacat cacggatatc tatgatggaa tgatgaggtt
      361 gttcgttgtc gatgtcgctc ttgaacggga taaagatgac cctcagttga tttttgagag
      421 catgaacagt acgggattgg ctttgtccca gacggatctt attcgtaatt atgtcctgat
      481 gagactgcca gttgagcaac aaaccagact gtatcagaag tattggtttc cgatggaaca
      541 aagctatggc aacgaatacg aattactgtt caacagcttc atgcgcgatt acctaaccat
      601 caagcaaaca gaaattcccc gcaaggatgg agtgtacgag gcattcaagc attttgtcga
      661 tacatgtggt cgaagcattg aggagattgt ggcagatatt ttcgaatttt catcttatta
      721 cagtaagatg actcttcata aggaggcaga caaaaatcta aatgaagctt tcatgcgttt
      781 atcccaattg aaagtggatg tctgttatcc ttttctgctt cctgtctatc gggattatgt
      841 tcaccaaatc acatctgctg atgaatttct cgctatcata tgccgggtgg aaagttatgt
      901 ttttcgacgt gccgtatgcg gaatccctac aaacagcctg aataaaacgt tcatgttgct
      961 atatcgccaa atcaatcctg agaagtatat ggagagcctg gatgcggccc tgatttcggc
     1021 ggataactat aaacgcttcc ctaccgacag ggaattcatg gaagcattgc tgtcgaagga
     1081 tgtctacaat ttcccgagac gtaattacct gttgtccatg cttgaaaaca aggacagaaa
     1141 agaacgcatc agcatcgggg actatacgat tgaacacatc atgccccaaa gcgctaatct
     1201 ttcctcggaa tggcagtcca tgttaggaga gcagtggcag gatgtacatg agaaatacct
     1261 tcataatctg ggcaatctga cgttgacagc atataactcc gaattgagta atcgctcttt
     1321 cagtgaaaag aaaacgattc ccggcggatt caatgattct ccccttcgtc ttaatgagta
     1381 tcctcgtcaa gtaaacaaat ggggtacgga acagatcgag gaacgggcac agaccctggc
     1441 acgaaaagcc tgtcaaatct ggatgcggcc agcacttcct caggaagttg ttgattccta
     1501 taagaaaaaa tcagcaccgg caccttcggt ttatagtatg gaaacctatg actggagtcc
     1561 tgccatgctg gaactgtttc atattttacg aaagagaatt cttaatcttg atccttccgt
     1621 tcgggaagtt tttctcaagc tttatattgc gtataaagta caaacaaatt ttgtcgatat
     1681 agttccccaa aaacgttctc tgaggctttc actcaatatt ccgttcaatg aagtgattga
     1741 cccggagggg atttgccgaa atgtcaaagg actggggcgc tggggcaatg gagaagtaga
     1801 aataatgatg aacgattctt cccaccttga aaccattatg gagcttatcc agcaagcctg
     1861 taatcgacaa atagaagaat agagtagaca caccaatcga tattatcgtt aaagacttag
     1921 caaattcact tccttgccgt acacaaaaac gattcttgaa atcgtctctg gacagcattc
     1981 catgcatacc tgcgcctagt acgaaaaaca tccagcatta aggcggctat gccagattct
     2041 tcattttcat aaagagtttt tccagttgat caaattttct ttttaaattt tcttttctca
     2101 ttgaggctat aatggttatt caacaataga tttctatacg gcgtcctggg catctgcatg
     2161 aaaccggata tctgcattgg tcgccatatc atcagacaaa tccaatagtt gactccgaca
     2221 tgaaactggc gtcaaaatag atgcagcccc tttctcaaca gccatttcca caactgccac
     2281 aggattgaat atttcttcga tggcccctgg attgatttct cctacagtaa agtaacaagc
     2341 cccccttgat gttttttcag caagaagcta accatgccga cctcgtatct catctggaaa
     2401 gaacgcctct aaacaggatc cacccctgca aacgttcaca cggctttgca tacgaagttg
     2461 tgtccagtac tcaggcaaaa aatcctccaa caccttgctt aacagaaaca atccggacgt
     2521 aatcagtgaa ggattggccc ttacggcatc cgttaaaaac cctccgccat cagggccaca
     2581 gcttcttcac ggctcggcat ggaacggatg gctcccttgc gtgtcgtcac aatggaagcg
     2641 gcggcattgg cgaacaaaag catgtccgcc aactggcctt ccgtcaggtt ttccagcccg
     2701 gtttccagaa ttcggtccag gcagcatccc aaaaaggcgt ctccggctcc tgttgtatca
     2761 atagtcttga cgttcaagta cgtaggctgg taaataagtt tgctttcata ggcagcccag
     2821 cttccttttc tgccccccgt caccagaata aggcgcaaat tgtcgaattc cctctgcaaa
     2881 atcctcaacc cttcttccat ggtggcgcag ccggtgagga agagaagttc ctccatttct
     2941 attttcatga cgctgcatgc gccgcatccg tagagcattt gttcccgggc cagttccata
     3001 tcaggccaca ggggcggacg gagattgggg tcaaaagaaa tgagtgcacc tttttcccgc
     3061 gcatggctga cggcatgccg ggtagccctc cgaacatcat catgcgtcat ggaaatcgtc
     3121 ccgaaatgga agacgcgcgc gtgctccact aattcaaggg ccacttctcc cgcagtcagc
     3181 ttcatatcgg cgccgggatt acggtaaaag gaaaattcac ggtctccgtg ttcgtcaatc
     3241 tgaacgaaag ccaacgtcgt attgacttcc cgggatgaga cgagccctga atctccgatg
     3301 ccttcttcct gaagcgtgcg gcgaagcatt ttcccgaaca tgtcgtggcc cactttgcca
     3361 ataaaggagg ttttgcgccc gagcctggac aacatggaca gaacgttgca gggagctccg
     3421 cccggattgg cctgataaac gggcagttcc cggtcattta ttccgcaggg agtgaagtct
     3481 atcagcaatt ctccgagagc tacgacgtca tacatgcttt tgcaaatgtt gaaagtgaac
     3541 cttccggcga aatataaacc gggagagata aagaagcata gaacgcgacg gccttgcggg
     3601 aaaaggaaaa agagattcat tccagtcatg gcggaaaagg aatttcccct tttatcccgc
     3661 cctgcagaaa cctcgaaggt tgatggagca tccggatgtg aataagtatg gcatatatcc
     3721 ctaacgccct ccactcagga aatgcccatc aagatgagac ggcaaaaaca gtcctgcgcg
     3781 aggcacgcgc ggaacgctgg agagagtgtc cccaacgggg caaatgcccc cttacgggca
     3841 tttatgggcg cgacttcttc tgctccctcc tattcctccg caacaaacac gcattttcaa
     3901 ggcatttcaa gcttctttaa gagaattcaa cccttttcaa gcatgtgtga tggtcacagt
     3961 attccgcatt tttcttcatg attcccttcc catcctgtaa tatgcgccca tgcaagaaaa
     4021 aagcagcggc tttcaatggc ccgcacagtg gacccccgag attctcgcca cgttgatgtt
     4081 cgtggtgcag aaaggaagga ttctcctgat acgcaaaaag aggggaatcg gcgcgggcaa
     4141 ggtgaacggc cccggcggaa agtttgaacc gggggaaacg gccctgcaat gcgttttgcg
     4201 ggaagttcgg gaagagcttc acattgatat tgaggatgcc cgggaaatgg gcgtgctgaa
     4261 tttttccttt gcctgcggca ccattccgga aatccgctgc cacgtgttta tggcgacgtc
     4321 ttttacaggc acccccacgg aaacccccga ggcggagccc ttctggtgtc cggtggacgg
     4381 gattccctat gacctcatgt ggcaggatga ccggttctgg ctgcccgcca tgctggacgg
     4441 gaaacggttt gaggcgtttt tcacgtttga gggggaccga atgctggaat tttccctgaa
     4501 agcagaggat taaatcgcca agaaacccca ggttgcggcc tcctcccttt aagccggatg
     4561 gggaacgctg aacttttttt attccgtcct tccccacgga ggcaacagat tcagaaggag
     4621 ggggaaacag tactgcccgg cgctccagaa aagattcccc gaaattcacc tgaaggtggg
     4681 gagcaaagag ggggcatttg cccttgaaga ccattctgaa cagcagctaa cgttgaaggg
     4741 ccggcttccg aaaaaaatgg accttccccc ttgtaccggg atacatcata ttcatctcct
     4801 tccctccagg tatcctgaaa cggatcatag gaattgctcc ggacgccagc atcgcagaga
     4861 tttccccatg gcgtccagaa aaaggaaagt tctccctgcc ggaaaccggt tccggcttga
     4921 ggttcatgcg aagggctgga attcagccaa atccgcggct ccgtaagaaa acggggggat
     4981 ggttgcataa tcggacggta atggaggttt aagggagcaa atcagctccg gcctttattt
     5041 tttccaagcg catccgaata tccactggtg catcaaatcc cttttccgta aacgggcgtg
     5101 ggcttttctt tttacccggt atccggaaaa tgctgatttt tcctatgttt tgccttcttt
     5161 ttcaggtaat gtgcggatgc ctgcgccctt gccgcttttt tactttgcac ggggaaagga
     5221 cggctcccgc ctccgggcat caggccgtat tctttaagct tttcctcccg cctccgttgt
     5281 gataatgata cgggagcaac cgtgtgatcc ttgatcgttc acttgctggc tatttcaatt
     5341 catattccat tattcaacat gtataccccc gaattcaaaa acatcctcac ttccaccctg
     5401 gacggactgc gtgcggaagg actttacaag gaagaacgct ttatcgcctc ccagcagtac
     5461 tcccaggtga cactgaagga cggacgctcc gtcatcaaca tgtgcgccaa taattatctg
     5521 ggccttgcta ataatccgga agtgatggaa gccgccaaga aagccatcga ccagtggggt
     5581 ttcggcatgg cgtccgtacg ttttatctgc ggcacgcaga cccttcaccg tcaattggaa
     5641 gaacgtctta cccaatttct cggcacggaa gacacgattc tgtttccctc ctgctttgac
     5701 gccaacggcg ggctgtttga aggcctcctg actgcagacg acgctattat ttccgattcc
     5761 ctgaaccacg cttccattat tgacggcgtg cgcctctgca aagccaagcg cttccgttat
     5821 gccaacaacg acatggcaga cctggaagcc aagctccagg aagcggacgc cgccggagcg
     5881 cgcgtgaagt tgatttccac ggacggcgtc ttttccatgg acggcatcat tgctcagctg
     5941 gacaagattc acgagctggc agccaagtac aatgccattg tccactttga cgactgccac
     6001 gccacgggct tcctgggcga aaagggccgc ggcacgcatg aataccgcgg cctgttcggc
     6061 catatagaca tcaccaccgg caccttgggc aaagcactgg gaggcgcttc cggcggctac
     6121 gtctccggcc cgaaggaagt agtggatgtc ctgcgccaga aggcgcgccc gtacctgttc
     6181 tccaacagcg tggccccggc tatcgtagcc gcttccatca aagtgctgga cctgttggaa
     6241 cagtccacgg aagcgcgcga ccgcgtagaa gccaatacca agtatttccg tgacgccatg
     6301 acgggagccg gattcaccat cggcggcaag gaccacccca tttccccggt catgctgggg
     6361 gatgccgtcc tgtcccagaa attctccgcc cagcttctgg acgaaggagt gtacgccgtc
     6421 ggcttcttct atcccgtcgt tcccaaggga caggcccgca tccgcacgca gatttctgcg
     6481 gcccataccc gcgaacagct ggacaaggcg attgaggcct tctgcaaggt aggtaaaaac
     6541 ctgggcgtta tttcctgatt cccgcgcttt ccatggcttc ggacgcatca cccatgcccg
     6601 gcggcaacct gccgacgctg cccgtcaagc gggtggtgcg tcccactccc tccattccgg
     6661 accatgaagt tgtgcgccag atagggagcg gcgcgtacgg agaagtatgg ctggcaaaat
     6721 ccctgaccgg ggcgtggcgc gccgtcaaaa tcgtatggag ggaggatttt gaagacgagc
     6781 gctctttcaa ccgggaattt gagggcattc tccagtatga acccatcgcc cgcaaccatc
     6841 cggggctggt ccatatcctg catgtaggga ggcatgacca ggattccccg ttttattatt
     6901 acgtcatgga actgggcgac gacgcccgca ccggagttca tattacaccg gacgaatata
     6961 ttccgcgcac gctccagacg gacaagaagt tttccggcaa caagcctctt cccctggatt
     7021 actgcctgga ggtaggcagc cagctggccc acgc
//
LOCUS       DAWEMX010000128         6911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4426_length_6911_cov_11.434947, whole genome shotgun sequence.
ACCESSION   DAWEMX010000128 DAWEMX010000000
VERSION     DAWEMX010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4426_length_6911_cov_11.434947"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1521 a         1991 c         1765 g         1634 t
ORIGIN      
        1 tgttattatt tctggccctt ggcggcgcgt acggctgctt cataggcggc aatgtccgcc
       61 cgttcctggc tgacggcagg atcggaaccg aacttggccg tcttatcctt cacctcattg
      121 accagggcac tgtcctccgc cgtcatcttc ttgcggttgg cgggagtatc cagcaactgc
      181 acgtactggc tgccggcctt ccaggcaacg tacctgtcgg aaggctgctt gcccgcaccc
      241 tgggacatcg tattcctctt ccacagcgta tccatctgcg ccagcacggc gggggcggac
      301 cacttcacga gccccttgta cgtacccgtg atgttcatga agttaatcaa cgccttgtcc
      361 agctcattct gcttcaggta ggcttcaccc tgcagataga ggacttccgg cagattcttt
      421 ttataggtct tgttttcgcg gtacttgtcg gcagcggcaa tgacggccgg ccagtcctgg
      481 cgggcggaag tgaacttgac cagattgtac tgggcgtctt ccatggcctt gcggtccggg
      541 ttggagctgg attcttcctt gctcagttca tccctcatca tcttgatggc ggtgtccacc
      601 ttggcgttat ccttgctgcg ccccagagct acggctttcc cgaagaccgc attcttgcgg
      661 tacttggaca tgccgttgat gcgggccttc ttacgcaccg ggtcaggttc cgacgggtcc
      721 aggatttcat caaagtagga gatgctttct tcaggctggg aagtgccggc caggtgcatg
      781 ccaagcttca caagcgtgta cgggggcaga tcggaaggct tgaagtcgcg cttcagctcc
      841 acgaagagct gcttgaccag accttccagg gcggctttct cggcacgctg ttcgtccgtc
      901 tccacaggag ccttttcata ccgttcctga gtctgggcaa taacggccat gcgaagcatg
      961 gcgctcagcg tcttgtcttt atcattgtcc acgccgggga accggtagaa gtggttgcgc
     1021 atggcattgg catccagatt gcggcccagg gcctgattgc cggccatgta cgtcttggta
     1081 taggaattga cggcctcttc cagaagaacg gtcatcttgg gatcgtcctt cttgccttcc
     1141 ctcacaataa tttcctgcat cttgacggat gccgggtcaa acatctcctt gtcatcctta
     1201 aagaagtcca tggcggcggc agccagctgg aggctgtagc ggctgccggg ctggtcgctg
     1261 tctttccagt actcgtcata ccatttcttg acctcgtccc ggcgcgccgt cctggccgtt
     1321 tccacggctt ccttcggttc taccggaaga ggcttcttgg tagcgttaat aaacaggttg
     1381 tacacggctt ccgcagcggg acgcttgtcg cccagcttct tggcggactc cagagcggcc
     1441 aggaaatagc cttccgcttc ctgctcatgc tccttgctgc cggcacacag attagccagc
     1501 aaattgttgg catacgggaa caggcggtgg tccttgaaat tgtccacaat ttccttcgca
     1561 tctttcgcgg caagctcttc gtctccgggg ttcttgcggt tcaggtagtt gaaggcccgg
     1621 tcatacaata cggcggccat attggcctcc cccttgtccg ggaaacgggc aatgtaattg
     1681 gtaaaggacc ggatggcttc atccgtattg cccaggttcg tgtaggtttc accaagaagg
     1741 tacatggccg ttttcagata agtgctgtca cggtattccc tcacgaagcg ttccagcacc
     1801 ggcaccgctt ccttataacg ggaggcgtcg gcagccccca ggttgaacag ggaggctcct
     1861 ttgcagtaca gggccagttc atagaactta tgggtggggt catccttatg caactccatg
     1921 accttgtccg cctgctgaac gcagagatcg aatttctttt ccttcagaag cccttccaaa
     1981 gacagcgtat taagcatggg ggcatacttg gaattgggga attccttcag gtgctgcgcc
     2041 acaagctcgc ctcccttgtc cgcttctccc agctgccagg tggtcatcgc cagataatac
     2101 aggttatcct ccctgcccgg agcatccggg taggattcat tgataagctg gtaggcggcg
     2161 cgggcaaccc tttgggaacc atgcaccagg gcctggttgc ccataaagct gaacgtgaag
     2221 gcgtccatga cctttccttc ctccttcatc ttgttgaact ggtcgatcag ggccgtcacc
     2281 ttcgctctgt ccagcgtatg ggaaaaatcc gtcacgccag gcacggcccc gttggcacgg
     2341 cccagacgat ccagggaata attggcatcc gccattactc cggactgatc gggaagaaga
     2401 cccatcagca ccatcgccag ggaagcataa tcttcagact ctttctgctt tccatcctga
     2461 agcagggaac gggatttctc cagcacttcc gcaacgtagc tcaccagccg cggggtgtac
     2521 aaagccacac gcgtgggact gatattcatc acggaagggt agttttccac aaacttcacc
     2581 gtctcactgc atttgccggt cttaatggcg gcatccacca tcgccagaaa gccattggta
     2641 ataacggcat ccgtaatgcg gcggcccttg tagcgtgacg tgacgcaaga ggtaaggttt
     2701 tccatccctt tttcaaaagc aggcggagac atcttgaaat agcattgggc cagaagcccc
     2761 tgcacttcgc cagcattgac gtttctggcg acaggatcgc tgcggtattc tttcaggaat
     2821 ttttccagca gctccgcacc cttggcaaaa tccccaagac ggcagtagtt ttccccttcc
     2881 cggaacaggg ccattttcac gagctggttt ttagcgctcg gaaacttggt gtaacaagcc
     2941 ttgaacgctt ccacagcctc cttgtaattc ttcagctggg ccagacagac gcccttctgg
     3001 taatagaacc agccgaagcc gtcatccagc cccagcatct tgatacgggg ttcataaacg
     3061 cggatgcagc gatccacggc atccagggct tcctgccact tcccttcctt aatggccgtc
     3121 agggccgtgc gctggtaatc gcgcgggcca tgctgggcgc cggatggaag aaccgccccg
     3181 atactcagca cggaaaccag gcctgcagcc agtgcagtag tatagtttga tttgatcatg
     3241 tgtttaaaat tcgttgaaac agaagagata ataaaacagg tgcaatatct gtgcaaatac
     3301 acggaataca cctatctgta atggattgcg tcattttaac aggatgcgtc aaactctaaa
     3361 gaagaaagtt ttgccttttt agtcatcctt gcaagaaagt tcttccatcc cgcgtctatc
     3421 cagagttgga acaggtctgt gccgcccagc cggaaaaata ctgctttttc aataccccgc
     3481 tacacactct gcggccaaag gaatgcactc tttccgcttt caaaagtttt tcggtggaac
     3541 acggcccact attccatcat acccttaagg agcctcggat cccgtctgtc aggatgccag
     3601 aggcacattt aacatggcgc aaacagatcg tccgttcaac cggaattcca tttttccggc
     3661 atccgcagcc atgcccgcct tctccccggc aacagccatc ccctcccctc tcagttctct
     3721 tttttcaaca tgaaatccca tccgcgagtt gaaagggaat atcgaaaagc agctattcct
     3781 acccacggga ggtacgggga aaaacacact tcctccccgt acttcctgat aacaatatca
     3841 cttcccggga atcatcaaca aggacttcct gttactgtac aaataagata aaggcttcga
     3901 gccgcctttc tccctgctct gctgcatcct ccttccaata tactttttcc atccctcccg
     3961 tgaatgaaca ggttttctga aggcggccct tccaatactc tcctgactgc ctctcctcct
     4021 cccagccaca cacggaatgc aatactggaa aaagacggca acatttctcc tgaagctccg
     4081 ttcccggacc gaattcacgg ctatcctccc gttacctgcg gtttcacaaa agggaaaaac
     4141 ggattcagca ccgggccaag aaaggagtat tgttccggcc aaaaccacat atcccaacca
     4201 gaacatttac tatacaataa aaagggtatg gtccggttcc ggatttctcc acagagcttc
     4261 cccccttgcc gcaaaatgct gccgtatgcg ggcctccgtt tctcccgtca ataacaaaaa
     4321 gcttccgctc cttttgggag cggaagctga gggtcaatga aaaaaataaa ggtaatttac
     4381 ttttagtcgt ccttaaggtc ggtcagagcc accgttgtaa tcccggcctc cgtcaacgcg
     4441 ttcatgacgt caataacgcg ctggtggggg acatcccctt ctacccacaa ctgcactacc
     4501 ggcttggtgc ctacagcgct ggcctcctgt tttttggctt ccagctgctg gacgagcgtc
     4561 gccaggttgt ggttgttcat gtcattgtcg atcatcaggc tgttgtcctt gccccagtaa
     4621 atggtgccgt ccttgataat gcggatcagc gccggttcaa tctgggaggc attttcgctc
     4681 acggactgcc ctggcatgga catatccaat ttgcgttcct gaatcagaga ggtcgctaca
     4741 atgaagtaaa tcagcagaag gaaacatacg tcgatgagag aggaaatatc cagcttggga
     4801 tcttcatcct caaccgtttc cagtttttta tgtcttgcca tagcgcgtaa tgttattcag
     4861 ccttggttgc aggagaggaa ttcttggcag gcagtacggc aaagatcact ttgactacgc
     4921 cgcattccgc agccgcgcgg attacttccc gggagccttt gaacagagcc tgctggtcac
     4981 cgcgcagata gagacgcgtt tcaaaatcct gcttgtcctt gaagcgttcc gcaaggtttt
     5041 tgatgtattc cttgagttca tcaaaggaat tgagcggagt gcctacgtcg gaggaccaga
     5101 gaacgctggg atctacgccg ggcggccgct tacccgcacc ttccccataa acattcacca
     5161 caatgcagcc gttggcggat ttcacttcac cggagctgct ggccgtgggc atctgaatgt
     5221 tcttatcctt tttaacggta atggccgtag cgttcaccac gaagaaaatc aacaggagga
     5281 aaaccatgtc tatcatgggg gacatatcca tttccccatt ttcttcttca ggcgctacat
     5341 tagctttttt cttacccata agctacattt tgttgaggtt ctccggcccg gttccgactg
     5401 agcggacgct tccgggccgg accgcctgtt ccggcaatca ggccaggcct tcggggatgc
     5461 gggcaagctg ggcttcggca ttcaccacgt tgatggaggt gttcaccatt tcctgcacag
     5521 tgcccacaca ttcggcaatc tgggcgttgg caatgccctt ctggaagaag aagaacggag
     5581 tggcgataat ggcgttgatc aatccccaga acgttgtcag aagagccacg gaaatgtcac
     5641 cggccagctg ggtggggtcc gcctggccgg cggtagccag cgtcccgaac gcgcccacca
     5701 tcccctgcac cgtcccgaac aggccgagca tgggagaaat ctgcgcacaa agggccagca
     5761 tgttgatcca tttgaacacg gaacggcttt cattggccgt aaagtcggcc acggcgtctt
     5821 caatagcatc tttgccgagg tcttccggac gggtagcgtc cacattcggc agggcgtagg
     5881 caaccagacg gcccaaatag gtgggactgc cggccgcaac ttcaatggcg gaccggatgc
     5941 ggcattcggc catcagctgc aacagagtga ccttgagatc ttccgggcag aatttttcct
     6001 ttttcagggc taccatgttg taaatcacca ggaaaatgat gaaagcttcc accagcataa
     6061 tcggaatcat ggtccaacca ccggcgatga tccatttgtc aagcatggtc tgttcctgcg
     6121 gggcaacggc ttcggcggct tcctgagcga aagcggatgt agaaaacgca gccatcagga
     6181 cggaagcaaa tgcaacgaaa cggaaaccca gtctaaaaag gttcttcata atttaagtta
     6241 tatggttgtt cgaaaagata ctggtgtatt aaagcatgca aattcagact tgcaagcatc
     6301 attttagacc ctgtaaaaaa ttcagacgcc tgcgcagccg ctctcccggg ccagccggct
     6361 gcagatgggg cagcgccccc cgcatccccg cgcagggcaa tattcacggc cgtacaagac
     6421 aatacgcaga tgaagatctc cccacaattt ttcagggaaa aggcttttca aatcgcgttc
     6481 cacggcttcc accgtttttc ccgtgctcag cccccacagc cgtgaaaggc ggaaaatgtg
     6541 ggtatccacc ggaaaggcgg gaacgccaaa ggcctgaacc atgacgacgg aagccgtttt
     6601 atgccccacc cccggaagag actccagagc agcaaagtcg caaggaacct ttcccctgta
     6661 tttttccacc aggatgcggc tgaggttcac gatggcggat gccttccttt ccgagagacc
     6721 gcagggcctc acaatttccc ggaccgccgc cacgtcctga cgggccattt cctccggggt
     6781 ggaagctagg gcgaacagag ccggagtcac cagattcacc ctcttgtccg tacactgggc
     6841 agacaagaga accgccccca gcagcgtgta agcgtcgcgg tgcaccaggg gaatgggagg
     6901 agccccgtac a
//
LOCUS       DAWEMX010000129         6856 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4476_length_6856_cov_11.621232, whole genome shotgun sequence.
ACCESSION   DAWEMX010000129 DAWEMX010000000
VERSION     DAWEMX010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_6856_cov_11.621232"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1562 a         2054 c         1849 g         1391 t
ORIGIN      
        1 cctcatcgac cgaacggcag gatacaactc caaaaccgcc tacctctacc agggggaaaa
       61 gcggggaaga acacaggaaa aaatttcatc aaaaaatacg gaactcctgt ccggacgtga
      121 gccggaatgc caataaataa aggataaaac gccggtcaca ctcaacaaca tgcctcaaca
      181 gcgttcaccg gaaaactccg ggttcctgta agcagtccac ggtttttcct tggcaggttt
      241 ccgcctccgg ctatcatgcg ggcatgggcc tttttcagga tcaaaccagc tctttgtcgg
      301 aaatcaagcg gcttgccgcc ctcgtcacgg atccctcccg cagggaggaa atcggttcgg
      361 atcaatggcc tctggccatg attgcctacg ggctggtcac ctgcaatgaa acgggaagag
      421 aagaagaagg cgtcaccatt tacaatatgt tccaatcccg ctgcgctccg gacgcccgcc
      481 gcaaatgcgc tctgcaactg gcagctttta tccggcagcg caaaggagac ggatggaggg
      541 ccctgctgcc ttttgccatg gcggatgagg cgccggatat tcgcaggcaa tccgctttcc
      601 ttatttatac gctggccgcg ccccagccgg aggagcggtt tcccggaatt gccggattgg
      661 cgaatgtcat ctgcgcatct ccctgtcccg gacaggcgtc catggctccg gcgctggacg
      721 ccctgatgag cctgggagac atgcggtttg ccccttattt gtctttcatt tccaaaaatc
      781 tccctcccgg gcgcctcgcg gacctgctgg ccgaaacgga ggccattccc acggacctgg
      841 gctgcggatg gctgctggat attctggatg aacatccgga gcttacctcc accattgcgg
      901 cggtactggc cgggatgccc tccagagccg gagaagtgct ggacgtggtc gttcccgtcc
      961 cctcctggca attcaccaat tcagccgtcc agccgctcca ttcctggagt attcccgaat
     1021 acaggctgcg catgggagaa cggctctcca gatatcttgc cccggaagag agggaagccg
     1081 tggaccgggc atggagctga gtcttccggt gtatcccaac ccgctgcatt tttcaaaaag
     1141 ccgtctccca aaaatgcgga acggctcttc tgattcggaa aatacagact caggggcgtt
     1201 ccacaaacag gcaaaaccgc aacatgagcc tgatgtccta caggctcata tcggcctccc
     1261 ctaggataat ttgcttcaag aggagaaaat cccccatggc gcgggttttc cccggccatt
     1321 tattcacctc gccactacgt ggcgatggtg gaaatccaca aagctccggt atgtctcctc
     1381 atccagcaaa tctccatgcg tcatcagcag gagcgggaaa ggttcaccct catccgtata
     1441 aggaggatgg atataatcaa actgcggatt ggggacaaag ccgtgcctct gataaaaccc
     1501 cagccgccgg cgtgagatcc cgtcctccgg aggatcaatt tccaaaataa ccttgtgcgg
     1561 aaacgcatcc ctcaattctt ccagaatcct ttgcccggcg ccctggccgc gcagggaggc
     1621 atccacggca aaatactcca gatacacgaa tttttcccga cgccacgtca ccagcatcgc
     1681 aaccacccgg ccttccccat ttaaaaatac ttcataacaa aaaccggggt tttgaaacgc
     1741 caacgggaaa tcctgtatcc gccgctgttc atgcaccgga aagcttgtcc ggtaaatatc
     1801 cagagaattc aggaacagcg gatcatccac ccccgccaca cggcgcttga tcagcggctc
     1861 agacttcatg gaccatatcc aaccacaccc gtctcctttc ggcaagtcct ccggaaagtc
     1921 ttccccagtc atacaaccag ttgttcaggc ttcaattgat gcagtgcttc ccaatcaagg
     1981 ataaggaaga aaaatacctt cgcgcggcga tacggagaca aggaaatccc cgtctgccta
     2041 ccgggactga cggcagcccc ggtacccata caagggatgc cgaaacaaag gcattccatg
     2101 ttctctttta tctctatgaa tttactttta cctgatgtat agaaagagcc tgctcacgtc
     2161 atgaattcca tttgaaattt tattccggaa acatgggaac ccgtctaaag ccatgatgcc
     2221 gctcgccaag atattcctgc tgctgatttt ttcctgggca gctttccccc gccctctcca
     2281 ggcccgggaa gagccgcccg catccccctc ctggccgtgc tgtgttcctg cacgccccgg
     2341 cagccgtatt ctctttctgg gaaacagcta tacgtttttc aaccgcatgc ccgccatggt
     2401 taaagccatg gcagacacca aaggattacg gccggaggtg cacatgcacg cggctccggc
     2461 agcatccctc cagtcccatt ggaatgacag gcgtgcccgc agcaacatgg agggaacggc
     2521 atgggacttc gttattctcc aggaccaaag cgccacacct atccgggctc cggaacgaac
     2581 catggagttc ggagaaaaat ggtgctctct gatacgagcc gccaaaggaa cgcccatcct
     2641 gatgctgacg tgggggaaaa gaggagcttc cggaactccg gacccgcagg agcaaaaggc
     2701 tcttcttgaa acgtatttga aacttgcccg gagggaaaaa gcagcgctcg cccccgtggg
     2761 cattgcctgg gagaattgcc tcaaacgcca tcctgacatc cagctgtacc agactgacgg
     2821 ccggcacccg acggaggaag ggagctacct gacggcatgc gtaatctact tcacgttatt
     2881 cggaaagtct ccgctgggcc tgccgggccg cttgtctttg aaagggacgc gactgagcaa
     2941 tcttcccgca gacagggcca gaatattgca gaccgtcgcc agggatacat gcaggcagct
     3001 cttttccgcc actgccggaa cccgtcccgc aaccgccgga ttgcagaccg ctgcattgtc
     3061 tccgcatagc gcaaaatcta ctcgcaaaac gggcacgggc cctttaccat ggcaacggac
     3121 atatatacta ttatgagcga acaagtcatt atcatcggtg ccggctgcgc tggttacacg
     3181 gcggccattt acaccgcccg cgccaatctc tctccgcttc tgatcacggg ttcccaaatc
     3241 ggcggacaac tgaccaccac gacggaagtg gaaaacttcc ccggctttcc ggacggcgtg
     3301 atggggccgg acctgatgtt cctgatgcag cagcaggcgg aaaagttcgg cacccggttt
     3361 gcatatgagg acgtcaagag cgtcgtcagg gatgaagcca ccgggctgtt caccgtgaag
     3421 accagcggcc agaattacga aacccggagt atcattgtgg ctacgggagc atccgcccgc
     3481 tatctgggaa taccgggaga ggaagggctg gttggccacg gactcaccgc ctgcgccacc
     3541 tgtgacggag ctttttaccg ggacgtgcct gtgtgcgtgg taggcggagg agacagcgcc
     3601 tgtgaagaag ccatgttcct gacgcgcttc gcttcccggg tgtacctgat ccaccgccgc
     3661 gacacgctcc gcgcttccaa aatcatggcg gaacgaacgc tgtccaatga aaagattttc
     3721 cccatgtgga attccaccat cgtaagctat aagacggacg acaagggaga actggaagcc
     3781 gtcatgctga aggacgtagt agaaggggat gaaacggaac ttcccgtcaa atgcgtcttt
     3841 atggcgatcg ggcacacgcc gaacacttct ttcctggggg atctggtgga ccgggacgat
     3901 gccggctaca ttatccggga aacagggatg atggcgacca ggacgccggg attgttcgcc
     3961 gccggagatg tggcggatcc tcactaccgc caggccattt cttccgccgg aatgggctgc
     4021 agcgccgcga ttgaagcgga acgctacctg ctggggctgt aaacccatct ttcggaacgg
     4081 gccggaattc ccagcgggat ttccggccct tccccgctac aggaaaacgg acatgcacca
     4141 ttacagcatt tttgagcttt tcagcatcgg cataggtcct tcatcatccc ataccgtagg
     4201 gcccatgcgg gcggcgcacc gctttcttga acagatacgc cactccccgc gcttggcaga
     4261 aatctcaggc atccgctgcg aatgctacgg ctccctggcc gccaccggac atgggcacgg
     4321 cacagacaca gccatcatgc tggggcttct gggggaagaa ccccatacgg tggagccgcg
     4381 cagcattccc ggaaaaatcg cccgcctgca ccagcaggaa acgttgtccg tcacggaaga
     4441 aaaaaacgtc cgcttctctc cttcccgcga cctggatctt tcccactacc agcccctgcg
     4501 cctgcatccg cacgggatgc agacccggtg gaagacgcgg tattttattc cacggggggc
     4561 gggttcgtct cttccgaaca ggaacttctg catccggaag aaagggaccg ggaaaccttt
     4621 ccttttcctt atgaatccgc ggaggaactg ctgcacatgg ctgatgagcg cggatgccct
     4681 ctttcctcca ttgtcatggc gaacgaatgc gccatgctcc cggaacggga agtccgtgaa
     4741 aaactggacc tgatctgggc gacgatgaag cagtccatct ccaacggcat gagcgcgcgc
     4801 ggcaatctgc cgggcgttct gcaggttccg cgccgggcca aaactttgcg caagaatctt
     4861 ctggtgcacg gagaatcggc cttgaaagac cctctctcca tcatggactg gatcaatctg
     4921 tacgccattg ccataagtga ggaaaacgcg gccggcggcc gcatcgtcac cgctccgacc
     4981 aatggagcgg cgggaatcgt tccggccgtt cttaactacg ccaccaaatt ctgcgtttcc
     5041 ccgtatccgg atgccgtgca tcgcttcctg ctcaccgccg ggggaatcgc catcctgtac
     5101 aagaaaaacg cctccatttc cggcgcggac gtaggctgcc agggagaagt gggcgtagcc
     5161 tgctccatgg ccgccgccgc tcttgcggag tacctggggg gaacacccca ccaggtggaa
     5221 aacgccgcgg aaataggcat ggagcacaat ctgggcctca cctgtgatcc tattgcgggc
     5281 ctcgtccaga taccctgcat cgagcgcaac gccatcgccg ccatcaaggc catcaacgcc
     5341 gcccgcatgg ccctgggcgg cacgggcgct cattttgtcc cgctggacaa ggtcatccgc
     5401 accatgctgg acaccggaag ggacatgcaa tccaaataca aggagaccgc ccagggcggc
     5461 ttggccgtca acgtggtgaa ttgctgaggg tacatgaacc ccctcccttg tcttttcacg
     5521 cgcgtctgtt cccgcaaaaa acgctccgcc ggattgacag atacggggag caaaggtagg
     5581 gtgcgggcat gctttcacgc ctttttcttg ctctggccct gttgctgggc ggcacgggac
     5641 tgtcccgggc cgttcacgtc gtcatgtgcg gcggccccgc actcaggcaa tgggaagggc
     5701 tgagaataca gcctgacagg catgacaact ggtgggccaa ctttgtaagg gcctccacca
     5761 tccgcatcgc ccagatccag cagcaagatc ctgccgccag aatcacctgg attgtttacc
     5821 gccccggtta cgcaacccgg gggaaggaag acggcaaacc ttacacccgc tggattgcgg
     5881 acctggcttc caagtataaa atcaggctcg tctgggtaaa cagcgccgac caggccatcc
     5941 gggcgctgaa ttcctccccg cgcttccacg gcgacaaggt ggaatccttt tactacttcg
     6001 gccattccaa ctgctatgcg tggatgctgg actacggcaa caatatcatg gccgtttcca
     6061 cggaatggat ccatgagacg gacctgaaca ggatacggcg ggatattttc acgccgcagt
     6121 ccgactgctg gagcttcggc tgctatacgg gagccagcat gtcccgctgg tggaaacgca
     6181 tcgtaggcgt ccccctctgg ggcaacacgg aatccacccg ctacggcccc gtctctgacg
     6241 gcaggctgcc ggaaggcgcg ggaaaatggg ttaaatagca ggcttgcacc gctctttcct
     6301 ttaaagacag gctccctaaa atcgggagcc tgttttgttg cctttcatgg aaagtgagga
     6361 tatgccggat tttcaatccg atatgccccg aaaacagtgc atgagtttct ctttctgcag
     6421 ggtgcgggac tgtttcctgc ggctgttgaa acggacattc ccggcagatg taccagcttt
     6481 cagggaaaag agttctccgg ttttcagact tatcttgagc agtctcaaaa gctggcggcg
     6541 gtttctgcac aggtggatat ttcggcaggc tttccgtatg cataaaaaag gttttcaatg
     6601 atgcaataaa gttcggcaca aattcttggt tatcagaatt tacgcttgac ttccggattg
     6661 aaaatccgct ttattcatca tgagcgggtt caagacagcc cggcaaatta cagcaataaa
     6721 aaagagcaac gaacaaccat gaagaacaca ttatttcttt ccggtcttat ggctttgtgt
     6781 agcggcgcag ggatggccgc caccgtagca atcgacctaa ctggggaggc agccgaaggg
     6841 gcatctctgc ctgcaa
//
LOCUS       DAWEMX010000130         6834 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4495_length_6834_cov_10.031126, whole genome shotgun sequence.
ACCESSION   DAWEMX010000130 DAWEMX010000000
VERSION     DAWEMX010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_6834_cov_10.031126"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5970..5979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1497 a         1914 c         1905 g         1508 t
ORIGIN      
        1 agcaggcttt ccccgatatg gaggtcgtgg cctgggtgga tcaggttcac catgtgaaag
       61 cttccgtgga ctggggagcc gtgacggcct ctgccattga gagcaacatc gtccgtacgg
      121 cggacccctc cgctgcggaa gccatgatcg ctgccatcaa ggaagctcgt gactccggaa
      181 actccttggg cggcgtggtc aaatgcgtgg tgcgcggctg ccctcccgga ctgggtgatc
      241 cggtttttga caagctggac gctacgcttg cccacgccat gatgagcatt cccgccacca
      301 aggctttcgc cgtgggttcc ggttttgaag cggcggacat gaccggcttg gaacataatg
      361 acccttttta catgcagggc tgccgggtgc gtactaccac caaccactcc ggcggtattc
      421 agggcggcat ctccaacgga gaggacattc tgatgcgcat cggcttcaag cctacggcca
      481 ccttgatgat tgaccagcag acggtcaaca gggacgggga ggatgcccgg ctcaagggca
      541 ggggacggca tgatgcctgc gtactgccgc gcgccgtgcc cattgtggag gccatggcct
      601 ggctctgcct gtgcgaccac tacctgcgcc aacgctgcca gagggctctg taacaaccgg
      661 tttcacggct tcttttcccc tcattcatgg attcctccct cacattttac ctggttctgg
      721 gcgcattgct gctgggtttc attgtactcg gcatgctgaa ggggatgatt aagatgctcc
      781 tgttcggcgc ggcagtgctc tcttccgtag ccgcttactt ctggctatcc aaatacggtt
      841 tcacctacct ctctttcatc accagcgacc cgcgggaatg gatggtcacg gtgctgtccg
      901 tcggaggcgc ggcggctgtc ttctttgtct ttatgcacgg tctcttctgg ttcagcgacg
      961 tcttctcctg gggacggcgc atgggcttcg gcggggccaa gggaatcgtc acgacggtac
     1021 tgatggcggc agtcgtatgc tgggtgggag tcatgtgcat cttcttttac ggctccatgg
     1081 cggaaatgac ccgcgcccga gaactggccc tgtaccacat ggaccccaac cgcaccattt
     1141 ccttacctgc catttattcc atcaaaaagt ccatcatgga caacccccac ctgagctggc
     1201 tggaaaaaat atctccggag catgatccgg aacggctttc cctggcaaaa atgatcgcct
     1261 acctggtgac gctcagtcca gagctctccg accagcggcg cacccagctg gactgctata
     1321 tgccccgttc ccgcattacc agccgcgtgc taagcctgaa aagcctgagc gaagaccccg
     1381 ccatccgcct tctggtgcag aagggggata tgcagggctt atataatgat gtcaagctga
     1441 cccgctttct ggaagaccac gatgtccgtt ccgtcctgtc ccaactggat gttgatcgtg
     1501 tgctgggctt tgtcacggaa attcgtcgga cggccccagc cggaaatgaa tacattcccg
     1561 aagccacgcc aattgagtga taagcgcgga ttttttgttc cgccggagtg gattcgttcc
     1621 ttttatccgc ctttaattca agaaaaaatc agggagttat tcccccttgc gcccgaaccg
     1681 gaaaaagaga gttcctgctt ttttcaggaa aaagcgcttc ctccagacgt tcatcccgga
     1741 agaagcgctc ttttgaggat aaggtattgc aaaccttgaa aaaacatcag gagagttttt
     1801 ccgcgatggc atccacccat tccgggaagg tatcatcatg gtctccattg gaaagcactc
     1861 ccggcagaag cctttgcgct ttcaaacgtt ccaggaagga atcgaagtcc ttgccagcct
     1921 ggcagaagtt ttcgtaaaat tcatctccaa gggcaaaaac ggcgtagctt acctcggaca
     1981 aatcttccga agagtccttc aattcctgat ggagggcctc tgcgttggtg ggaggttctc
     2041 cgtcccccca cgtactggtg atgaccagca gcgttcccgc attcttcaac tgttccaccg
     2101 ttataccttc catgtcaacc acctttgcct gatggccgag cttggtcaat tttgcccgtg
     2161 cgtcctgggc cagactctcc gaattgccag tttccgttcc gtagataata gtgatttcca
     2221 tgcgttctga tgattggttt gacaacgaaa gttagattaa actaattaaa caggcaaggc
     2281 aaaacagcag gacgacggct gaatttttct tccttgttta tccttcaggg caactggttt
     2341 tccggaaaat tctccgatac catactactc tcgtttgatt atcaaagtat tcggatgctg
     2401 tggaaaggtg aagcaccagc cgaaaaggcg cgcaaaacga tagtataatc ccttgcggca
     2461 ggaattctgg ctggtcatga tatcgtaaaa agccaggtca atctggcttc tggaaatggg
     2521 ggctccattc attttggcgt gcagcatggc gtcgtgaaac agggaaggga ggagcatatc
     2581 cggaggggtg gacccccagg tgacgccgtc ccagccatag ccgggagtaa tgctgaaacg
     2641 accgccctcc gcctgcatgc gtatggatgg gtcatacacg ctgacggctg tccagcggcc
     2701 aaaatccgcc ctttttcttc cgctggtcca gctgatggcg tttttagtga tgtagaaagg
     2761 cttgtcttca tagagggttc cttcatacca gccggccacg ggttccgggg ccgcagccgg
     2821 agtggagcag gattccagaa acataatgtg aataagaaat aaagggcatg aggggacggc
     2881 gcgttatccg gatagttgtt tcttattgga tttccagcac gatgatttcc ggagggcagc
     2941 agaagcgcag gggaagaacg ctgcatccca aaccgcgggt aatgaggaag ggggttccgt
     3001 tcagctggga ccagggatag gaaaatttga ccttctctcg tgagatgttt gccagggccc
     3061 tgcctccggg aaggcatatc tggcctccgt gggtatgcgc gctgatgacg aggtccgccg
     3121 tgccgggagc cagcagagga aaaatgtcgg gtacatgtga aagaaggata tgcggaatat
     3181 ccggggaaaa acgcagaggc aattcctccg gcctgattct caggtggtag tcgtccctga
     3241 cggaagagag ctgcagctca cggcctccgg gaaggtgcag cagcagggaa tcattccaca
     3301 tgggcaggat gcccagcccg gaaaacatgt tgcgccataa gttccagccg taatactggt
     3361 catggttgcc ctgcacggca aaaatgccga gaggggcttt caacatcttg aaatattccc
     3421 gggccgtttc cggggccatg ctgctctccc tcgtgtggcc gttggcataa tcccccagca
     3481 ggaaaatcat atccggccga gcttccatga ttgtttccat gtacctgtgc gccagatcgc
     3541 ccccgttcgg acgcagatgg aaatccccgg caatcgcgat acggagggga gggctttctt
     3601 ttctcttcca ctgaggcatc tccatcgcag ccgtgacggt ttccacacgc ctgggttcca
     3661 cctgccatgc ccacatgaat aaggagaagc cgatcatcaa tattgggaca aagataattg
     3721 ccaatttgtt gagaaagggg cggcgtggtc gtgacttcat gcagggcagg gctcctgact
     3781 gattatgagg ccttttaacc gattttcaat cattcgtgtt tgctaatcgg gaatttgctg
     3841 gtatgatccg tcccgctcaa ggtagcggct gtccgatgac tgcagctcta tcttcatatt
     3901 aacatcctaa taccatgcag tttacacacg aaatcgaaca aatgtgttgc gtcaagaaag
     3961 gctgcaacca tggtccggca cctatcccgc aggaaggcaa ctggacgcaa tccagggaaa
     4021 tcacggatat ttccggcctg acccacggcg tgggctggtg cgctccccag cagggcacct
     4081 gcaaactgac gctgaatgtc aaaaacggca tcattgaaga agcactcgtg gaaaccagcg
     4141 gctgctccgg catgacccat tccgccgcca tggctgcgga aatcctgccc ggcaagacca
     4201 tactggaagc aatgaacacg gacctcgtgt gcgacgccat caacaccgcc atgcgcgaac
     4261 tgttcctgca gatcgtgtac ggccgcaccc agagcgcctt ctctgaagga ggccttccca
     4321 tcggcgccgg tcttgaagac ctcggcaagg ggcttcgctc ccagatcggc accctgtacg
     4381 gcacccgcgc caagggcccc cgctatttgg aaatggctga aggctacatt accaagcttg
     4441 ccctggatga agacggtgaa atcatcggct acgagtttgt gcgcatgggg ccgatgatgg
     4501 aaatgatcaa gaagggcgtg gacgccaacg aagccgtcgc caaatgcacg ggcaactacg
     4561 gtcgtttcga cgaggccgcc aagtacatcg atcctcgtca cgaatgagat tgaattaaaa
     4621 agagtatctt aacttcaagc actattacca ttatgcctct tttcgaatcc tacgaccgcc
     4681 gcatcaagca gatcaatgaa gccctggcca aatacggcat cggctccatt gaagaagcgg
     4741 aacaactctg cctttccaag ggaatcaacc cccgtgaaat tgtgaacggt attcagccca
     4801 tcgcgtttga aaacgccggc tgggcctatg tggtgggagc cgccattgcc atcaagaagg
     4861 gctgcacgaa ggccgctgac gccgctgaag ccatcggaga aggccttcag tccttctgca
     4921 ttcccggctc cgttgcggat gaacgcaagg tgggcctggg ccacggcaac ctggccgcca
     4981 tgctcctgcg cgatgaaacg gaatgcttct gcttcctggc cggtcacgaa tcctttgccg
     5041 ccgctgaagg cgccatcggt attgccaagt ccgccaaccg cgtccgcaaa caggatctcc
     5101 gcgtttgcct gaacggcctc ggcaaggatg ccgcgtacat catctcccgc atcaacgggt
     5161 tcacgtacgt gcaaaccaag tttgactatg ccacgggcaa gctgaacatc atccaggaga
     5221 aagctttctc caagggtccc aaagccgccg tgcgcgtgta cggctgcgat gacgtgcgcg
     5281 aaggcgtggc tgtcatgtgg aatgaaggcg tggatgtttc cattaccggg aactccacca
     5341 accccacccg cttccagcac ccggtagccg gcacctataa aaaggaatgc aatgagaagg
     5401 gcaagaagta cttctccgtg gcttccggcg gcggcacggg ccgtaccctg cacccggaca
     5461 acatggcagc cggtcccgct tcctacggca tgaccgatac catgggccgt atgcactctg
     5521 acgctcagtt cgccggctcc tcctccgtcc ccgctcacgt ggaaatgatg ggcttcctcg
     5581 gcatgggcaa caacccgatg gtcggcgctt ccgtagcggt ggccgtggct gttgaagaag
     5641 ccgccaagca gaacgcctga gcgagcagct gagcaaaaaa tcgcagagcc gctttcccga
     5701 tttgggaaag cggctctttt agcaattgaa agaaaaaaag gggatgtccc ccatttccgg
     5761 cccccttttc ctgcccttgt atccgcatca gcttccgata cggaatcttc catcccggtc
     5821 cgcccgggga ggaagcccgc gcgaaggaac ggattttctg gaaacggatt cctgcctggc
     5881 agaggccggc ttgacggagc ggattttcgg gcgggaggca gaagcgggag caaccgtttt
     5941 tcccggttgc gtgccgtggg aggaaggaan nnnnnnnnnc cgctgttctc ccggtctcag
     6001 ggaataatcc gcctttttgg gagctccggc tttttgcgcc agaggttcca cgcgccgctg
     6061 cagctgagcg gcggtatcca gtttgccggc cacccattcc gggttccatt ccggctcgga
     6121 tgttttcagg gactgaagca actgctccgc tttcttgccg ctttccaggg ctgccttata
     6181 atccccctcc ttctcctgcc gtgccgcgcg cgtcagggaa aggttggctt cgtaaaaagt
     6241 gtctccggga ttgccaaggg ccgttgtagc cgccaggaac atgcagagca gggaacaggg
     6301 tagaaacttc cttttcatga ttgttggcac gataacatga ttcctcgttt tggaaaggcc
     6361 tttcttccgg catgttttgc gcagggccgg actccgtttc ccttgcggat aaaggaaagg
     6421 ttctccattc cgcaccccgg agcaacttga cggaaagtcc ggagtgccgt atgctggacg
     6481 ggacctgcta tatgacccgg aacgagccat catcccttcc agctccgcag atggaggaag
     6541 atcccagatt cctgacggaa caaattgtaa cctatttggg caataagcgt tcccttcttc
     6601 attttctggg gacggggctg gaacaggtga aaaaacgcct gggtaaggaa aggctgaaag
     6661 caggggatct tttttccggc agcggcattg tggcgcgttt tctgaaacag cattcggaag
     6721 acttgatcgt caatgatctg gaggaataca gccgggtcgt caatacctgt tacctgagca
     6781 acgcggagga agtggaaaat ctggagctgg acagacatta cagacgcctg ctgc
//
LOCUS       DAWEMX010000131         6730 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4567_length_6730_cov_14.174232, whole genome shotgun sequence.
ACCESSION   DAWEMX010000131 DAWEMX010000000
VERSION     DAWEMX010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_6730_cov_14.174232"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1893..1902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5810..5819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1233 a         1812 c         2034 g         1631 t
ORIGIN      
        1 ggcggaagaa acgggccact ccaggtaact ccattctgcg gctaccgtcg cggacgtcca
       61 ggttcctata attgatccgg ctgcttttct tttcagggaa gaaggaagag ggggcgcctc
      121 ccggacggcc tggcggcagg cttggagcaa atcctctggc gcatgtgcgg cgcttacgcc
      181 ggtggaacgg acccattttt cctcccatgc acccatatcc ggatttttgc cggaaagccg
      241 gctttgcatg aaatgttccc agcgcggcag gtaatagtcc cgaataaggc cgctccatat
      301 tctggaggcg tagtcgtcta tgggagggcc ccacctggtc accagccgtc tggcgttttt
      361 ttcatattcg tctttttcct ccagagaagt tccgtgagac cgggcaaacg atatccagcg
      421 cgacagcctc caggtgggat gcccttccag aagggaatct gccagaaggg cgtatttgcg
      481 gaaataggcc atgcattttt ccgcattttc ctgatcctgt tcgtccagag cttcctgggc
      541 ggcccggatg gcttcattca tgcgtatgcc aagataatgg gctgccatct caacggcatc
      601 ctggcggaac aggggggatt gttccaggcc gcgggtgttg acgaacaggg agagtccccg
      661 gagaaagtct tcacttgtgt caacggaaca gtttcgggtg ccgggtttca tttgccagtt
      721 gaaacgggga tgatctttta ggttggagta agccgtacag cggaacagcc tccaggcttc
      781 cttcagggta tccggataat tcccgtatct ggcccggcaa taattttcca gatattgttc
      841 cacgttttcc tggcggttcc tccatgcggc atccgtgatc agttcgtaga tcacatcgtt
      901 attttctatt ccttccgggg ccatgcccat tcctgaaaga cgccccctgg ccggagagtt
      961 caaggcttcc aggtggccgt tggcgtaaaa atccattact ccggtcatgg cgcatttgcc
     1021 gcccatgttg ggaaccacgc tgtaaaccca cggtttattg aaaaacccct tgaaaacgtc
     1081 ccagttcatg ccgttgcgcc agaaggtttt gttgtaatcc gccgccaggt ccagaaggag
     1141 cattttatcg tccggaaccc tgctgagcaa ggccttcaga gtatctgcgt tccagatgtt
     1201 gcgctggtat ccgaacatcc agccctgcat cacccatacc gcgtccggat tgtcggagga
     1261 aatggagcgg tagatttgtt ccccaaggga gctgagcatg ctgttgcggg cttcaatgcc
     1321 tcctttgttt gcagggagtt ccatttcatt gaaggagtct gcgagaaaat aggtgttttt
     1381 cctgaattcc ttctgccatt cctgcatgta caggcgtcct attttgagga acagaggctc
     1441 ttccggagaa agaaaatggg catggttttt ttggggccag cctccccagc ccagccggtg
     1501 aagcttcgct tccggataaa ggcggaggat tccccgggga acgaaaccgg aaaaagcggg
     1561 gcagatgggg gacatgccca gggatttcat cctgtggaga atgcggtgct gcagggcgat
     1621 ttgttccttg tgccagctgg cgggaagggt gccgtcatga ttgacgatgt ttcccatccg
     1681 ctgccaggga agatgggccg gccccgtgta aaattcttct atttcttttt ccgtcagccc
     1741 cagttgcttc cagacccgga cggcgatccc ctccgtggca accagagcca ggggcatgtt
     1801 gatgccatgc agagccatcc agtctatttc cttttcccag cgttcccagg tccagtaggg
     1861 catcgtgtat ccataggtga ccacattgaa atnnnnnnnn nncgcggcgc ggtgtccggc
     1921 agctgtgccg gccagcgaat gtccttgttt tcccaggata ccattcccag acggttcgtt
     1981 ttcagaaaat catagaatcc ccggcaaagg gctgtgccgc tgcttccccg gatcgtcagc
     2041 actcctccgg aggctttcgt ctcgaaaaca tcatatccgt cttctttttc aatggtttcc
     2101 aggtgcagtt tttccgggga ggcggccaga gacggcacgg ttcgcatgat gactgcgcgg
     2161 gcggcttccc gttccctgtt tttctgcatg tcttccgcga atacatgcag gcttgccatc
     2221 atcagaaaga tcagaattct gggaaaggag gagaccatgc ccgtaaatac gcagggaggg
     2281 tcattgtcct ttcaggggat ggaaatggca gttccgcgga atccggccca atgaaaaggc
     2341 ccctgcatgt caacatgcag gggccctggt tgaaaagcct aaaagcgttt attccgcctt
     2401 gggtttccgc ggcgttctct tgcgcggcgc tttgggcctg gctggttttt tgtccttgat
     2461 gtgcaggaag gtgagcgtct ccgtcccgtc cgtttccacg gcttcaattt tatcgccggg
     2521 tttcacgtct cccctcagca gggattccgc cagcgggtct tccagcaggc gttcgatggc
     2581 gcggcgcatc gggcgggcac cgtactgcgg gtcatagccg ttcttgatga tcaggctcag
     2641 agcttcctgg ctcaaggcaa gcgtgatatt cttggtcttg agacgggaga tgagcttgtc
     2701 cgcttccaga tgcacgatgc gttccaggtc gtcccgttcc agcatgcgga agacagaaat
     2761 gtcatcgaag cggttcagga attccggacg gaattgcttg cgcgccgctt ccagaatctt
     2821 ttccttcatg gcggcgtagt ccgcgttgtc ggcggccatg gcgccgaagc ccatggtgct
     2881 ctgccgtttg gcggaagcgg cccccacatt ggaggtcagg atgatgatgg tgttgcggaa
     2941 gttgatcttg cgtcccagcg agtctgttac gctgccttct tccaaaatct ggagaagcag
     3001 gttcatgacg tccggatggg ccttttccac ttcatcaaag aggatgacgg agtagggcct
     3061 gcgccggacg gcttccgtga gctgtccgcc ttcgtcatgg cctacgtagc cgggaggaga
     3121 accgatgaga cgggacgtgg tgtgtttttc catgtactcg ctcatgtcca cctgaatcag
     3181 ggcgtccgag gtaccgaaca tgatttccgc cagattgcgg gccagatagg ttttgcccac
     3241 gccggtgggg cccaggaaga ggaatgagcc gatcgggcgg cgcggatcct tgatatccgc
     3301 acggctgcgc cgcagagcgc gggcgatggc ggaagccgcc tcgtcctggc cgatgacttt
     3361 gcttttcaac tcttcctcca tccgcagaag cttggtggtt tccttttcct ccatccgggc
     3421 cagcgggatg cccgtccatt tggcgagtac gctcatcacg tcttcatccg tcacgggaac
     3481 gtagttggtc tcataggagt tgcgccaggt ttccagcatg ttctgaagtt ccgccttggc
     3541 ttgtttttcc tcgtcgcgca ggcgggctgc ttcttcaaaa agctgggttt ccacagcctt
     3601 gtccttgcgt tccttgattt ccgcaatgcg gatttccatg ttgctgatgt ggtccgggcg
     3661 ggtcatctgg gagacgcgct tgcgcgcgcc cgcttcatcc aggatgtcaa tggccttgtc
     3721 cggcaggaac cggcccgtca aatagcggtg ggagagcttg gctgcggctt caacggcttc
     3781 cttcgtataa tgcaccttgt gatgttcctc atacttgggc tggatgccgg caagaatctg
     3841 gatggtgtcc tccacggagg gttcccctac ctgcacctgc tggaagcggc gttccagggc
     3901 ggcgtccttc tcaatgtgtt tcctgtattc gttgagcgtg gtggcgccta tggcctggag
     3961 ctcaccccgg gagagggcgg gcttgatgat gttggaggcg tccatggagc cttccgcgga
     4021 gccggcgcct acaatggtgt gcagttcatc aatgaaaagg atgacattgc cttcccggcg
     4081 tatttcatcc attacggcct tgagccgttc ctcaaactgg ccgcggtatt tggtgccggc
     4141 caccatcagc gccaggtcca gggagatgac cctcttgctg cgcaggagct ccggaacatg
     4201 gccctgggct atttcctggg ccagcccttc cacgatggcg gttttgccca ccccggcttc
     4261 accgagcagg acggggttgt ttttgttcct ccggcacaga atctggatga cgcgctcaat
     4321 ttccgaggcg cggccgatga cgggatccag cttgccgtcc cgggccagct gggtcatgtc
     4381 ccggccaaag gcctggagcg cgggggtctt ggatttctgc tgcctggagt aggaggggga
     4441 ggaaggctcg tccgtcggga tggcggggga ttcattttcc tcctcgtcgt catcgtcgtc
     4501 cgggatgatg ttgtcttcat ccgcatccat ctggtatttg ggggtcagca cgtccagcag
     4561 ttcgcgccgc gcctgttcca aatccacgcc gaagtggcgc agaatctgtc cggccacgcc
     4621 gtcgccgtca cggatgaggc ccaggagcag gtgttccgtg cccacatacg tgtggttgag
     4681 ttcctgggct tccctgtttg ccattgccag gactttgcgc accctggggg tgtacgggat
     4741 gttgccctcc gcctggggtt ccgtgccccg gcctatttgt tcctccacct gtttgcgcac
     4801 ggcggtaagc tccacaccca gattttccag gatggtgacg gccacgccct tgcccagctt
     4861 gagcaggccg agcagaaggt gttcggttcc tatgtaatga tgattgaagc ggtccgcttc
     4921 ccggcgcgca tgcgccagca cttgttgcgc cctaggcgta aaattattca tggatggtca
     4981 aaaaatttgt atggttgcat tagttgccgg aaagttcttt ccgaataata tcagcgcgga
     5041 tctggtccct gtgttccgga gtggtttggt ccgtggtgga caggcgggcc agatgggccg
     5101 gctggatgtt catgatggtt ttattgacgt tttttaatgc ctggtgggaa aagatgtcca
     5161 gagacgctcc catccggagg aaggagagca gggccagcgc ctcctgcgtg gaaagcagag
     5221 tggcgttggt cagaaggccg taggcgcggg aaacgcggtc cttcacctgc agggaagatg
     5281 actggagcag ccgcctgcgt gcgttccatt cctgatgggc caggtcggag gtgaagcggg
     5341 tcatgcgttc cacaatctgg tcttcactgt cccccagcgt ggactggttg gatatctgga
     5401 aaaggttgcc ggtggcttcc gtcccttccc cgtaaaggcc gcgaacggta atgttgagct
     5461 gcgcggccgc ctgaacgacc tgctgcatct gttcggagat gacgaggccc ggcagatgca
     5521 tcatcacgga ggccctcatg cccgtgccca ggttggtggg acaggcggta atgtagccca
     5581 gccgcgtatt gtaggcgtac gggagcctgg cctccagttc ggagtctatt ttggagatgg
     5641 ccccccatgc ttttttaagc tggatgccgg gcaggatata ctgcaggcgc agatggtctt
     5701 cctcattaaa gaggatgctg gcattctggc tgcgggagat gaggacggcg cagccttccg
     5761 aacgcgccgc cagttcccgg gaaatgaggt ggcgttccac cagcaactgn nnnnnnnnnc
     5821 ccagccgggt aagggggtgg cggtcagggt gcgcgccagg cgtatgcggg aggtgaggac
     5881 gatgtcgtgc acatcccttg attccaccat ccatttggcg gggttgttga gtaagtcgtc
     5941 aaagagcatg tatgttaatt ggtcggcgtg cgcaattgtt tgatttgatc gcggagtttg
     6001 gcggcgcgtt cataatcctc tcttgccact gccagggaaa gctgttgttc cagatcgctg
     6061 atggtatggg tttccgtttc gcgtttttcg gcccgttccg gcgttttccc gtgatggtgg
     6121 gaatcaggct ggatctgggt aagaagaggg aggatttctt cttcaaagac gttgtagcat
     6181 tcactgcagc ccaggcgtcc tacgtttttg taatcctgaa gggtgaagga gcagatcgga
     6241 cagcttgtga ggggcaggga ttgcagggcc ggaatgggag cggcgggccc cgccgcgcct
     6301 tccttgccga attggccctg aaggttcggg aacagtttct ccgccagatc aaaggcatcc
     6361 gggtccagca gaccccgctc ctgggcgcat ttggcgcaaa ggcagaattt ggtggacttc
     6421 ccgttgatga tctgtgtcag gaacaccgtt gccggtgcag agcatatctc acattttttc
     6481 acaacccgca ttataatggc gctccctgtg gcggactagt agtctgcgtg tcatggattg
     6541 tcatgaaaac gggctttgtt attgtacttt ggacgaattc tttccgtgct gttttaaaag
     6601 cccgcaccgt gatgaataca aataggctgt gcaaaaaata atgcccagca ccagaatgat
     6661 ggttgcaccc agatgccagc ccagggggaa ggacaggtaa aaagccagca gggagcccag
     6721 tcctccgatg
//
LOCUS       DAWEMX010000132         6709 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4587_length_6709_cov_9.006613, whole genome shotgun sequence.
ACCESSION   DAWEMX010000132 DAWEMX010000000
VERSION     DAWEMX010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4587_length_6709_cov_9.006613"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2934..2943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3667..3676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1706 a         2005 c         1560 g         1418 t
ORIGIN      
        1 ggggggccag aatatccatg ctcacggtgc cgatgatgat ttcactcacg aacaggcagc
       61 cggaaaaagg ggcatggaac gccgtcgtga acccggctgc cgctccgcag ccaaccagca
      121 gacggagccg cggaatggat acgtgcagac gctgccccac ggtagacgca atcagggcgg
      181 aagactggat gataggccct tccttgccga tggccgcgcc ggaagcgatg ctccatgccg
      241 cggaaaaaac tttcagggcg gaggcccgga acttgatcag cccgttcccg atggacatgg
      301 cctccatgta ttccgtagcc tgccccggca tccttttctt caggaacagc agaatgcagc
      361 cggccaccag gccgcccgcc accggaataa acatgcgcca ttccggctcc accagggaaa
      421 aggttcctac gcgggaatcc gaccagatgc cggtgagcat ccactggatc acttccacca
      481 tccactcgaa aaacagagac atgcaggagc ccaccatgcc gacgagaacg gcccatacat
      541 aagtaacctg cctttccccc accttccaat gatggaacag ttttaaaatc catccgtacc
      601 agacggaatc cggcggctgg gactggggga gtggagaatt catagaaaag aaggcaatac
      661 tattccattg tgacaaaaaa atacctccgg ctgttcgata cggaagaaaa aactgcccag
      721 ccccctgccc ctccggagcc agagaagatt ttcaacggat acgcagacgg ctcttacggg
      781 cccccttcgg ggcctggccc ccgcgccggt agtcagaacg tatatttcaa attgacgccg
      841 taatagaaat catcccccag ccccttttgc tttaccacgg tcagcgcctg gctgtaattg
      901 gtgaacaatt ccacggtcag gcgcccggta gcacggaagg taacgctcag attataatcc
      961 agagcattgg aaccatggtg caccaccggg cctacatacc ccccattgta accgtagcgc
     1021 actacggcat ccacccgcac gcaatcagct atttcttcat gcatccccgc aaatacggca
     1081 taataaccgc ggaacgtatg gttgttatac tgccagtaca ggtccgcgcc gccaaaccag
     1141 cggtcggaaa aatggtaggt cgttttcaat ccgggacgcg ggattccctt atctcccgga
     1201 aaaacaaagc tgtgctcata ccagggcatg accgtccagt tgcccaattc cctgacgtag
     1261 tccagccggt atttcagctc cgcatgattc cggtggtccg tggaaacgcc gtaccatatt
     1321 ttccccaccc actctttata ctgtatttca ggaacaaaca tgcagagaga accatcccac
     1381 aagtccaagc cctcgcactc gtacttgcta gcccaccccg cctgaaatcg caaataaaca
     1441 cgctcatccg caggactggc cagcgaaagg gggtgggggc acgccgtctt caggtggtag
     1501 gactgttcct ggcagaccgc cgtcgcccag gctccgggac acgccagcat gccggtgcag
     1561 gccgccagcg acaacagaat tacggtattc atcttcatat tggtcatggc tggaaatttt
     1621 tctgaaacgg catatccgga agcggcgcca tccacccatg cgtacccgca tcccgcttta
     1681 gccgcaaacg gatactacgc ccgcacccgg acaaatacct tctgaagatg agaccagcat
     1741 gaaaaaacaa tattcagttc caagagccaa aaaaacgaat tccccatgaa agattgtgtc
     1801 agcagccttt gtacctataa ccgtgtccat gaaattcatt atccccgcac tcgccctggc
     1861 cgcctgcctt cccgcagcct tctcacaaga acaaatcatc ccgaaaccgg cggaaatcac
     1921 attgttcaca ggaagtccag cccgtctcac accggattcc ctcatcatca cggaaacaca
     1981 ggataaagct ttcctggatc aggcagggca attgcagcag atgctcagcg cagggacggg
     2041 actccccctt ccccttaaac cggccgggca aacgtcgaaa aaagccgcct gcattgtcat
     2101 caaaaaagac ccagccctgg ccgccagggg agaagaagcc tactccatcc aatcatcccc
     2161 aagtggaatc atcctttccg cagccgatgc caagggcatc ttttacgcgg ggcaaagcct
     2221 ggtccagatg atgccctccg tcttccacga ccggacggcg aataaatccg ccgtccggtg
     2281 gaatatttct gaaactccgt tccgcataac ggactacccg cggttcacct ggcgggcgct
     2341 gatgattgat gaagcacgcc acttctttgg cgagaaaacc attaaacaga tcatcgacca
     2401 aatggctctg ctgaaaatga acatcctgca ctggcacctg acggacgaca caggatggcg
     2461 cattgaaatc aagaagtatc cgcgcctgac ctccatcggc tccaaacgca gggaatcaga
     2521 aatcggcaca tggaacagcg gcaagtcaga cggaacgccg catgaaggct tttataccca
     2581 ggaacagatc agggatatcg tacaatacgc agcccgccga aacatcacca tcgttccgga
     2641 aattgaaatg ccgggccatg ccagcgccgc cgccgtagca taccccttcc tgagcctgaa
     2701 aactcccggg gaagtgccca caacattcat cgtcaatacg gccttcgatc ccacctcgga
     2761 aaaaacttat gccttcctgt ccgatgtttt ggatgaaatc acggcactct tccccggcag
     2821 aatcatccac attggcggag atgaggtgcg ctatgacaag caatggaagg gtgttccgga
     2881 aattgaggaa ttcatgaaaa aaaacggcat gaaaagttat gcggacgtcc agannnnnnn
     2941 nnngacgtca atggggacgg aggaggaaaa gccggcgcca aactggatac aaacgccgtc
     3001 atccagttct ggaagggcaa caccggcctg gctaaaaacg ccatccggga cgggcatgac
     3061 gttatcaatt ccctccacac ctccacctat ttggattaca gctacggcag cattcccctg
     3121 caaaaggcat acggattcga acccgttttc cccgggttgg aggaacagta ccaatccaga
     3181 gtcaagggac tgggcgccca agtatggacg gaatggattt ccacaccgga acgcctgcac
     3241 taccaggcat tcccccgtgc ctgcgccttt gcggaagtcg gctggactcc cgccggtaaa
     3301 aaggattttc cggatttcaa aaaacgcttg aaagcgtata gcgagcgtat ggatctgatg
     3361 gggatcaagt ttgcccggaa cgtcatcagc caaatagaca aatctgactt tttcaatacg
     3421 cccaggatcg gcacatggac gcctgccacc ctgacccggg aggaacattc gtttgacgtt
     3481 accaaactgg tcaaagcgtc cggcaaacac accgtcaccc tgctgtacga caaaggcgcc
     3541 cacgccatcg aaatcgaatc cgtagccctg tatgaaaatt cccgggaagt ctcccgggac
     3601 gcccatgcag gcagaagcgg cgcccataag gaaaatatcc agtacattct gaatgccccg
     3661 gcccccnnnn nnnnnnccca ggcaaggagc aacctatacg gtcaaagcaa acttcaaagg
     3721 ggccggaggc cgggattccc acgggacagt gtattttgaa acgccataaa aacaccgccc
     3781 ccgccccata ccagaaacag caggggggct tcacacagag ccccctttac tccaacaaac
     3841 ggagcacagc atccgcagac tcatggttac ggacacgagc ctttcagtat cattggcctc
     3901 ccaagggcgc cggcatgtct tttcatagag gaagacgccc ctttccatca ggtggcacga
     3961 aattgatact ttaacccaca gaaaggctta ccctgaaatc agccttgcct taacaaggct
     4021 ccggaggtcg ttgcttgcga acctccggaa cctgaacaac cgtgaatcgg cgtaagatga
     4081 ttttcatccg ggaaagccag acggaattta catgttatca tctaccactt tttccacata
     4141 ctggaaaaag atgcttccgt cctccccgct gattccatag tctctgacat cctcaatatt
     4201 cgcattcttg attcgggtaa taatatagac tttggagtac aaaatccccc attccccata
     4261 ctttttctta tcaaaccaga tatggtggtt atagtatggt tcaaaataga tggtgcctcc
     4321 gtctatcgga tcggaaatcg ggccggaata tttgacaaca atatggctga ttcccttgta
     4381 acaccattcc ctgccgcatt gaggattttt ggaaagggtg gtgctcattt caatggtggt
     4441 gtccatgaca tcataggaca acctatcctc atcatggtat ttgattcctt tttgaggcaa
     4501 attgaactga tttttgatga tgactccatc ataaatgaga cgtccccctt tccattccgt
     4561 cttctgcggc actgtttccc ctttcaagag acagattcct gagagagaaa ccattattgc
     4621 acaggcaatt ttattcattg ttattttcta ctttgttgtt gtttcagcaa ttccttattg
     4681 aggaattctt tcatttcctg aacagttttt tcaggagggg tcttcgcatc gtcccgatac
     4741 tgcctccagt cgatgcgcag cttggccatc aggcgcaatt cgctgagaga aaaaggaaca
     4801 gtacccgggg cattcggcat gaaatcccgg gacctgttgc caatataata aatctggctc
     4861 tgctcccaag aacgcttcat ggtctcatcg aaggaagctc tcacacggtc gtcgcttact
     4921 ctggcttccc actggaggca gtccgccagg caaccaccct cggcccgact cttccgggcc
     4981 tgccgccaca gggaaagaat atacctgttc tgaagactgc acgcagccgg agtaaggtac
     5041 ttgcgcgtat actgcaactc atacagaggc ttgcacacag ccttttccct ctgtcccaga
     5101 gtagctccac tcaccctttc cggagtgggg acaataccgg ccagagcacc ggatacgtaa
     5161 taaggcagtt cctcaggcat tcgaatttcc cacggtattt tttcattcac aaacacgcca
     5221 tttaaaatgg tattaaagga accgttccgg caaatacgga acaggtaaat cccccctttc
     5281 ctgctcatga accttttata aaccccggac cccgcaaatc tggaaacgcg tgtgcgcgcc
     5341 tccaccgggt aggaatacca ctgctccatg gatgcagcaa gttccttccc ccaattattc
     5401 tgttctccta tcgcaagatc cccctcaata ttgaactgga acacaagggg agcggtggaa
     5461 gggtgccttc tggcttccac cacgtaatcc cgcctggatt cattggtgag ataaccgttg
     5521 ggattgtaaa aatacagggc tatttcatgg acaccttccg gcacctctac ggctacccat
     5581 atatcgggtc cgtccacaaa gcccggatac acttctccgt ggtcatccca ctccgcctct
     5641 gtccgcacgg aatccgtagg gtcgaaaaga acgttgcggt tatcattttc cttgatgctg
     5701 tgcacccacc agcgcaaagc gtcccctttc ttgcggttga gccccatcag cccctgaatc
     5761 cagtagggag acaaggcccc cttgtatccg ggatgtccgg gaacggaaga aagagtacgg
     5821 aacttaatag gcttggcttt gaattcggaa ttgctcatgg gggcattcgt ggcgcacagc
     5881 gtggcacgcg tgtgcccgta cttcccgcac cagtcccctt ttgtagtcca gtcctccccc
     5941 cggaacacaa tattcttccc cgcatgcgca tcggacccca gcctttccaa ccttctgagc
     6001 accacagaag gctcctccat ctgcgccgga cgagggaacg ggacttcatt gtccatctgt
     6061 tccctgtcca gtttatacgc cagagtcccc tttttcagac gaacaaagcc ccgtccgtat
     6121 gtcgccgccc aaacactccc gtcctgaagc gccagcaagg aaggaacatc cacgttttcc
     6181 agccccttcc ttattctctg attcaccgtc attttttcag catccagaac atccactccc
     6241 tgcatccggt agccgatgaa aatttccttt ccctgctgga taatggaaga aataaaatct
     6301 tcagacaaca ttctggaatt cgactccccc ggcaccgcag tcttccgtgc ggcagaacca
     6361 tacagatggt tgtttttctg ggcataatca cgccctctca tataacgcca agaactaatg
     6421 ccgttactgt acgccaagcc tgagcaggta cctaccaaca cctggccctt atccgtacaa
     6481 gtcaaaacat tgcagatatt ggaaggcaaa ccatcaccat acggctggta cggctgacgg
     6541 gcgaattgcc tgttatccca gtaccaggga gcctgaacat ttttccactg catatacttt
     6601 gactggcgcg aagagcagga taccccgccg caagcatagg ccagccacaa attccccctg
     6661 gtatcaaaac ctgcggaagc cgcctggtct tccaccaggc cggtggacc
//
LOCUS       DAWEMX010000133         6617 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4660_length_6617_cov_11.983694, whole genome shotgun sequence.
ACCESSION   DAWEMX010000133 DAWEMX010000000
VERSION     DAWEMX010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_6617_cov_11.983694"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3210..3219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5869..5968
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1383 a         1684 c         1937 g         1503 t
ORIGIN      
        1 ccatgtgggc aatgcggaac gttttgttct tcaagtcccc gtaaccgttg ccgagctgca
       61 tgccgcgttc aatcagggag ttgttcaggg cggcgatgtc aatgtccctc gtgttgctga
      121 tcaccgtcag ggtgcgggaa aggtgatccc ggttggcaaa gacgctgaaa tattcatctg
      181 cccaggaacg cacgaattcg gccatggctt catgacgcgc aaagcggttt tccacacctt
      241 cctgcatgat gcgctggagc tggaggtcca tggcgtacat gatggagatg cagggagtgt
      301 tcgtgtactg gtagtccttc ttctggatgg tgtcgtaaat ggaacgcagg tcaaagtagc
      361 agccgcggtt gggaacggag gcggtgcgtt cgtacgcttt ttgggatagc gtgcagacag
      421 ccatgccggg gggcagggca agcgccttct gggtggaggt caccagtacg tccacgccca
      481 gcttgtccgt ttcaatccgg gagccggcgg cggaactcac ggcgtccatg caccacacga
      541 catccgggta ttttttaagc acttccgcaa tttcctccac cgggttctgg atgccggtgg
      601 acgtctcatt gtgcgtgatg caaatggcgt cgtatttgcc ggtggagagg gcggcgtcca
      661 ccatttccgg ggtagtgggc tgccccagtt cgctcttgaa aatatcggag ggcacgccgt
      721 tgcccgtagc gatcttgtac catttgtcgc cgaaggcgcc cacggaaaaa atggcggcgc
      781 gtttggcggt gcaggaacgc acggcgcctt ccatcaggcc cgtaccggag gaagtggaaa
      841 gcagaatttc ctgttctgtc agcaggatgc ggcgcagatt gttggaaatg cggcgctgca
      901 gggcggaggc cgctttggat cggtgtccca tcattgggcc gttcatggcg gcaagtactt
      961 caggtgctac ttcagtgggt ccgggaatga ataatttggt tgccatttgt ttttaagaac
     1021 tgggtacgca tactatagcg tctccaaacg gtttgactat gaatttttgt tgagccttac
     1081 cgggaaaacc tgccgcggca gcggagaaag caaagagagg atgtctgtat tgggcggcac
     1141 agatggccag ttgggagtaa tgcgggcggg atgcggcgct ccgcatgttc cgtcatccca
     1201 tgccggcatg gttcctccgt tgatcctttt caaccggatt ttctggcagg aaatcaggat
     1261 ggaacgaggc ggtgaacctg tgctgaaacg gttctcacgt tctgccggtg aaacggcttt
     1321 tttctcctgt tcggaaaggc agggcgcagg ccttgccctc tttccgcagg aggagtgttt
     1381 ttggaattta gaggctgatc agcttgacca gccagtaacc tccgaagaaa agccagatat
     1441 acaggaagcc tgcaagcagg aaaggccgcg ctccggcttt cttaaacttc tcaaagctgg
     1501 attccgcgcc cagggccgtc atcgccatgg tcagcaggaa tgtatccagg gtgtttatgg
     1561 cgtccacaag tccggcaggc agcaggtgca gggaattaaa gccaatgacg gcaaggaaca
     1621 ggaaggcgaa ccagggaatg gtaattttcc gtttttcccc gtttgcgccg gaagaggggt
     1681 ccctccgggc caggacaatg ctcaggacga ccagcaccgg ggccagcatc attacccgga
     1741 tcattttgac gataatggcc ggatcggaaa tagcttgccc catggcgttg ccggcgccta
     1801 ccacatgcgc cacttcatga agggtggctc cggtaaaaag ccccatttgt tccggcgtca
     1861 gatccagtat tccggcccgg tgcagggctg gatacagaaa catggagagc gtgccgaaga
     1921 tgaccacggt ggaaacggcc acagcggctt tgtaaggttt gcttttgacg acgggttccg
     1981 cccccagaac ggctgctgcc ccgcaaatgg aactgccgat ggaggtcagc agggccgtgt
     2041 ccctgtccat tttcagcagg cgccccagcc cggctcccag aagaatggtg agagtgacca
     2101 caataaggtc gagcgccagg gcggagccgc cgatgtcgat gacactttga aaagtcagtt
     2161 tgaatccgta caggacaatg cctgttctca aaacctgctt ggtgcagaac tgaatgccgg
     2221 gaacccatgt ttccggaagg tggttgcgca ggctgttggc gtaaagcatt cccagcacga
     2281 tgccgacgat cagcgggctg agggaaagat gtttcaccca ttcaaaatcc gcaatgtaaa
     2341 aagcggaaaa ggaaaacaga acaataagca ggatgccgtg gaggggattg gctagatgtt
     2401 tgggagacat ggtcggtaaa tgatggttgc ggctgcccgt tgtatctggc cgggagcgta
     2461 ccggattggc tgcggaatga tatacgatgc aatcattata aaatctaatt aaaaattatt
     2521 atagccaatt aaattttgtt ataataatat cggcaaaact tgatgaacag ctcattgatt
     2581 ctgctgtggt ctccatgtcc tgagacgaag tggaaggtgc ggcggatttc caggtggcgg
     2641 agatgtattt tcttcaaact tccgcgtttg aattctcccc ggacggcaag ggaggagagg
     2701 aaagagcacg ccggagaatg ctccagatac atcttgatgc tttccgtatt gcccagcgtc
     2761 atcagaatat ggaggccgga aacggagatc ccgtgttttt tcagcgcttc ctccaccacc
     2821 cggcgcgtgc cggaccccgg ttcacgcatg atcaggggca catgggagag ttgcgggagg
     2881 gaaagccctt cactgggcac ggcggcctgc atgcctcctg ccagaataat ttcgtcctcc
     2941 ataaatggct ggtaatgcag gtccgcgcgc gttgccgtgc cttccacgat gccaagctgc
     3001 acggattcat cctggacggc ctgttctatt tcctccgtat tgccgctttt cagccatatg
     3061 cgtatttccg ggtaatggcg gttgaacagg gccagcagcg gaggcagcac atactgggac
     3121 atggtggagc tggctcccag gctcaattcg ccggaaacag gggaattgcg gccaaaggat
     3181 atttcttcag ccagcgtttc gtattccttn nnnnnnnnnt attccttgag gatgcggcgg
     3241 gaatgggcca gcaggcgcac gccttccgcc gtcagggcaa tccctcgtcc ctcccttttg
     3301 aacagacggg catccagcag acgttccagt tccttgatat gccgggtgac ggccggctgc
     3361 gtgatgaaca gttcttccgc cgcgcgcgta aaattcaggc gccgtgcgac ggtttcaaac
     3421 actttcagcc ggaaatccgt aatcatggag aggaaataat attcaaccat gccgccgggg
     3481 ggacggcaag ctcctttcct ccgcatgatg gcgtacggcg ctgggaatgg cgctcaagcg
     3541 caagacgcgc gggtgcccgt aatattccgg aaccgtacat cctctgaacg gcaggtggcg
     3601 gaagagagtc caatgcctat atgaaactgc attccatgat tattgccggc ggagccggcc
     3661 ttttgttgtt gggggcctgt tccccctctt cctatcagac cacatttccg gaaccggcct
     3721 atgtaaaggt ttccaccaat aacggagctt atgctcctta tccggacgat cctgtcggcc
     3781 agcaggaact ggtgtactgg tataattccc ttgccgctcc aatgacggtg gttgcggagg
     3841 ttcctcccac tcccattgtc acgacaggtc cggggccggt tgttcttgat tccagcgatt
     3901 tctccgcgaa attgtaccgc gacagaacgg aaatcaaaaa gaaggataag gacggcaatt
     3961 gggttaccag cgtgcgcccc accacccccg cagaccagcg catgaggaat tacgtgatgg
     4021 cttccaaaaa agcccgttag atttttcctc cgttgacgaa agacctttcc ggcctgcgtt
     4081 cctctccgga ggaagcgggc ccggcttgtt tacgggcaga tttttcttcc cattcttccg
     4141 ggcaaatggg gcgccggcgg ataatttttc ccggccaggg caaataggcg tccgtgtaaa
     4201 aggtggaacc gcaaccccgg cattccagca tgatgcctgt atccggcctt ctttgttctt
     4261 ccggagtttg cggagcagaa taaagcccgg cgtttccgca gtgcgggcag cggatgggaa
     4321 ggggaatggt gtgttgaaca atcagcgttg ccgcgtaagc gagtgcccag gcaatgtagc
     4381 ccgcccagaa aggccattcc ggatgaagca gccagactgc tgcaacaagg agagcggagc
     4441 acaaggcata cagaaggctc cgcttctgcc ttccggcaag gagatggaag caataaaccg
     4501 tatcctgcat aaaaaggaag gagatgaatt tacaggtcat ccagcgtcca ttcatccttc
     4561 cattccagga taatctggtc accctcccat ttcatgggca gccacaggta acggccgtca
     4621 atggcgtttt tgggccgcca gatgtccgcc atgaagatga attttccggg tttgccctgt
     4681 acaggcagga tgaaagtgct ctggccgccg aacgtgatgc ctgccttggg ccccacgcag
     4741 ggatttttca attccatcca tgggccggcg atgtttttgg ccacggcgct gcgggcggcg
     4801 ttgggagccc agcccgtgca tccggaagcg ataagatagt atttgccctt gtgtttgcaa
     4861 atagcaggag cttccatgta gcggttgatg aacgcccgga catattttcc cgtatgcccc
     4921 gtgtaatccg gcgtcagctc cgcgatgtga agggtaatat tgccttccga ggaatagatg
     4981 tggtaggcct tgccgtcgtc atccacaaaa atggtcatgt cccggctcat ctgcccgccg
     5041 ggatagtccc tgcggatgat gcggacgtag tcgggcattt ccttggtgaa ttcggtcgtc
     5101 tgatcctttt tatccatgtt caggggccat ttgcccgggt tgactcgccc ggctttcagg
     5161 aacttgaatg gccctgtggg ttttgctgcc gttgccaccc ccgcgtaagc ggatctatat
     5221 cctttcccct tgagttccag gtggaaccac atcacgtact ttccggtctt tttgttgtaa
     5281 accactttgg ggcgttccag gatgcagcct ttggcgatgg ggtgggaatg atccccctcc
     5341 accaccttca gggcgattcc ttcgtccttc cagttataga ggtctttgga ggaatagcag
     5401 tgaaccccta cctgggcata gtttcctgcg tccccctcca ccttgtgttc tccgtaccag
     5461 taataggttc ccttgtcgta caggatgccg ccgccgtggg cgttgatgtg cgcgccgttg
     5521 ttgtccgtcc agatttcccc gggtctgaag gaggtgtgtt taattggggt ttctgctgcg
     5581 gcaacggaga aaaggcagca tgccagatta gcggaaagga tggctgttaa tattttcata
     5641 tgtattggag gtaaacgtac tacatgtctt tcagcgtcca ttcgtccttc cattccagtt
     5701 tgatctggtc tccgtcaaac tggatgggaa gccacatata gcggccgtca atggcatttt
     5761 gcggtcgcca gatgtccgcc atgaagatga attttccggg cttgccctgc acgggcagga
     5821 tgaaagtgct ctggccgccg aacgtgatgc ctgccttggg ccccacgcnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg atgccgccgc cgtgggcgtt gatgtgcgcg
     6001 ccgttgttgt ccgtccagat ttctccgggt ctgaaggaag tatgtttaat tggggtttct
     6061 gcggtggaga cggaagccgc actgtaaaac aggcagagcg ccaaaagcga tttcatggta
     6121 tttatcatgg ccgggatagt gtggaaatag aggatggaga aggttccggt tggccgtaca
     6181 gcttgtccca ggcgcgggaa agagcgtaca gggcatatcc ggtgccgtag aatttgtggt
     6241 aattatacag ggggtagtcc caccaggccc cgtctgttcc ctggcgggtg agaatatcgt
     6301 ccgccaacag ggatgcatga cgctttacct ggtttggagc cagcatgtcc aggcacattc
     6361 ctgaataata gtatccgtaa tagaaaaaat agccggaatt ctgggcgaaa ctctcgtggg
     6421 gagttggttt atgcagggcc atcgtcagcc atcccctgcg gctccacagg cggtccaggc
     6481 cgtccacaag ctggcgcagg gagatgtcct ccggttccca gagcctgatg gcgtaatcac
     6541 aggcgggagt ccgggccagg ctgccggtat ggcggttgat gggcctgccg ggatagaagg
     6601 aatgggaaag ggaataa
//
LOCUS       DAWEMX010000134         6525 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4747_length_6525_cov_11.360587, whole genome shotgun sequence.
ACCESSION   DAWEMX010000134 DAWEMX010000000
VERSION     DAWEMX010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4747_length_6525_cov_11.360587"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2395..2404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1495 a         1817 c         1842 g         1361 t
ORIGIN      
        1 aagctgatgg aactgacttt tcaggaacag gaaaggaaaa tcaacgacgc ggcttttgcc
       61 aagtacaagc cggagctggt caggctcatg aatgaatgcg ttaaaaacga ggattttgaa
      121 caggccaagc atatcaggga tattctcgaa cggaagtccc tgcccggcat tcaggaagag
      181 gaaaaagagg attactcgca tttcatcggg caatgggaag aaacggcggg aggatacgtt
      241 tatttaagcc gctttgatgg gaagcaggcg caacagaagt ccggcaatac cggatggctg
      301 ggcaaaggcg gagtggacaa ggatttttca tcccctgaca tcatcaggct caatgaaacc
      361 cggacggtat ggttctactg gatcaaaacg gaccgcgacg accatatttt ccaggttaac
      421 cacgctacca aactggtttc cctgttaaag cggaagggag cgcgcgaaaa agaagcgaat
      481 cgttccacct attcctcatc tttccctaaa ttcgaccttc tttctgaaaa aatccatgcc
      541 gatatggcaa ccggcaaagg aaaactggcc cggcaatacg ctcaagccct gcaaaaaaag
      601 agcagggaat ggggtgtttc cggtcatttg gaagcggcca tctgggcgcg caaacgggcc
      661 gagggattgc aaaaggggaa tcataaggag aaacagcaac tccaggacat caccggaaca
      721 tgggacttcc cttcaggcac cgttcaaatt aaggatccga aagaattccg aatggagggg
      781 gaaagcatga cgttcgagta tctgagatcc ttaaatgaca gtgtccacct gttctatatc
      841 caggaaacca aacagaagaa aatggtaacc cggattaaca acctgcttct cattatccct
      901 tatgactgcc gcgggccatt ccaggaaggg gttatgaaac cctgagcttt tccgtaaaag
      961 agcctctctt ttacgggaag cccatttcag aaaaccgcaa cccatgcctt tcccgaatca
     1021 gggttaccca agcccacccc cgtctgtcga tgattttaag gccatcagcc tgggcagagc
     1081 cgtgtattca cggccttttt tcatcaggcc gccggcgctc accagattct ggagtttttc
     1141 gtaaatcctc agcctcagga tttcctctcc gcccagggac atgtgctttg ccttcatcac
     1201 gacaacggta cgctgaaaaa tgtcattgaa ttccagcatg cccgcttcat gaagaatgag
     1261 aataagctca tcagtcacca aatccggtgt cttgcgcgag gaacgtgatc tcctatcctc
     1321 cagcatgatg ataggatatt atcatgacac acttccaacc gtcaagttga taaaatttgc
     1381 gtcattttcc accgacatac ggctcaaaaa gcaccaaagg cgaagggtcc gacagcgaat
     1441 gaggcctgtt ccggaaaccg gaaatgagtt gccacccctt ctctccggag gacttactta
     1501 tccggaagaa gaaaagattc tctgcaagaa aagggaacgg ggaacacgta ttacctgcag
     1561 cccgggcagc aatacggagc tttttacaga gatgaaagga aataaacggt tcattttcag
     1621 gttatgtgca accgcagcgc tttttctgag cctttttccc ctgcaggccg gagaatgcac
     1681 gctgatgagc tataacgtaa agaacggcac cggcatggac ggcaggaggg attatgaccg
     1741 cacggcccgg gtaatcgcgg aggaaaaacc ggatgtcgtg gcccttcagg agctggacca
     1801 ggggacaatc aggagcggcg gcagggatac cctgcaggaa ttggccgcaa ggacaacgtt
     1861 gacgggtacc tatgccaagg ccattgatta ttcaggcggt tcctatggag taggcatttt
     1921 atccagggaa aagcctttga gcgtcaaacg gattcctttg cccggcaggg aggaggcccg
     1981 tgtactgttg atggccgagt tcagggatta ctggttttgc gtcacgcatt tatccctgac
     2041 cagggaagac agcagcgctt ccattgatat gattgccgcc ctggccgcaa aatgcagcaa
     2101 gccctttttt attgcagggg atttcaattt aacgccggat tcggaaccga taacccggat
     2161 gaaaaaatat ttcatcctgc tgagcgatcc ggcccaaaaa acgtttcccg ccgggcatcc
     2221 gaaggaatgc attgattaca tctggatgta caggggaaaa aagacggagg cgttccatgt
     2281 gaaggaacgc agggtaattg aagctcccgc cgcctccgac caccgcccgg tcaaggtgac
     2341 ggtcagctat tgacatgatg cttccggccc gtcaccgggt agcccgcggg gcatnnnnnn
     2401 nnnncattga cgccgcccac cagcagcacg atttcccccc tgggggggcg ttccgcaaat
     2461 tccgccgcca gccgggaggc cgaaccgcga tggtaagttt caaaggtttt tgtcagttcc
     2521 ctggctacgc agacgggaat atccggatcc aacgccgcca gagcctgcac ggttttgacg
     2581 atgcgatggg gggattcata aaaaatactg gtatgggaag cttccactgc cgtttggagc
     2641 acagcgtttt ttttcccgga tttgacgggc agaaagccgc cgaagaagaa tgcgtccgta
     2701 ggcagtccgg accccaccaa tgccgtcacc acggcggatg gcccgggaag gacggtgaag
     2761 cttactccct tttccttgag ggtctgaatg agacgatagc ccggatcgct cacgccaggc
     2821 atgcccgcat cactgatcac ggcaaagctt tctccgccgg ccgcgcgcgc cgcaatttcc
     2881 ccggcacgcg gctgctcatt gtggtcatgc atgcttacca gcggtttgcg gatttcataa
     2941 tgggaaagca gaagaccgga atggcgggtg tcctcacacg caatgacatc caccttgcgc
     3001 agcacgtcca gagcccgcag ggtgatgtcc gcccggttgc cgatcggcac cggaacaaaa
     3061 taaacgctgt attccacttt ctccataagc acagattttc ctgaaccggg cgggattagc
     3121 cattggtaag ggtttccgca atggaagtag ccaccagacg ggccgcatag gaaagggcat
     3181 tcgtacgggc cgactgaatg ttgccctggt caaagaattg acctacttcc tccgcagagc
     3241 cggcatacag gatttcctgc gtcctggcgt ccaccacacg gtatttgaca gacaggcgca
     3301 acccaagttc cgtactcttg taggtgtctt cccggctgga tcgcaactgg tcgaaggtga
     3361 tggagtacac ttccccctcc acccggatat ccgcatccgc accggagctt aaccggtagg
     3421 tgccgtcccg ctgcagctca tccgccaagg cgccggtcac cagcgtggcg gcacggggtt
     3481 ccaaagagtt gttggtaaag aggcatacct tgacggtctg ggccagttcc agcttgggcg
     3541 gcttggtgcc gccaagctgg tagccgcaag aaccggccag gaggcaaaaa acgggcagga
     3601 agagtttgaa aacgttccgg atacggttca tgaggacgga gagttcctgt tatctgctgg
     3661 tcacgcgcag ttctttcagg cgggccttcg ccctggcggc ggcggcggga ttgatgctgc
     3721 ctttggaggc tacttcctgg tagcagaaga cggcggcatc cgcatctttc atcttggtca
     3781 ggtagtattc cgccacttcc agctgctggg ccaccagctc acgcttcatg gccgccagat
     3841 cggcacgggc ggctcccgca cgggcgctgt tgggataacg ctgaaggtag tcttcatacg
     3901 cttcctgagc gcgggccaca ttaacgtggt tgcggtctcc gtccgcagca gcctggcgat
     3961 acagggtggc tatctggagc tgggccgtgg gggccagcgg agagttgggg taattgtcca
     4021 ccaggttctg gtatgcctct atggcctcct tcatccggcc gcgggcggcc agataattgc
     4081 ccagcacgtt catggcctgc ggggcggtag gggcatacgg ggcgttgtcg cgcacatgct
     4141 tcagccattc cgtgacgttc gtgggatcca tcctgacgtc aaacatccac agcacgcggt
     4201 tggtcagcgc gccgctcgca gcgccaaagg ccatttcctt ctgccgggac atggcctgcc
     4261 tgtacagggg gctgtccggg tggcggtcaa tgagtttctg gtactggtca aacgcatccg
     4321 caggttcctt gcgggcctca tagagttccg ccattctgaa ccgggcctgg ggagcttccc
     4381 tggaaagggg gtgtttttcc accacgcgcc tgtatttctt gatggccccg gagagatcat
     4441 tcttcgcttc ctttgccctg gcttcctgca tcagggcaat cgcctgctga tccaccatgc
     4501 ggacggttcc ggggggagga ggcgcttccg aagagcattg gcacagggcc agcgccccgg
     4561 aaacggcagc catcatgaga aacagttttt tcacattcat gattttacag cagaaaaaga
     4621 gggcgcgtca atccggtgaa cgcagattga cgcgcaaaag gagttaatgg cctttgccgt
     4681 acacggcttc ggcatcaaaa acgacgcctc cgtccgcttc acgcagcgtg acggcccccc
     4741 cctggggctg ggctttcagt tcatcgccga acggaacgga acggtaaaag caggagtggt
     4801 gcccggtgtg gcaggctccg gcgccgcact ggtccaccag cacaatcagg gcatcctggt
     4861 cacagtcaac caggatttgc tttacttttt gcacgtggcc gctggtagcg cccttatgcc
     4921 agatttcctg acggctgcgg gaatagtaga cggcttcccc cagttccatg gtcatgcgca
     4981 gggattccgc attcatatag gccagcatca gcggttcatg ggtaatatgg tccatggcga
     5041 gggccggaat caggccgttc tggtcaaatt tgggggcgaa ttccacgcct ttttccacat
     5101 tcaccttgcc gcggtcggca aagacaatcc gggatgagat gtcattttgt tccataaccg
     5161 gcccccttag tacatgaaat atccgacata taccaatctt ttctgttgcc ctgacgcaac
     5221 gaaatgtttt cataggagca tatggcattt acggcatccc aagcgttcga tctgctgcgc
     5281 accgccttct cccgcaaccg gcttccccat gccctgctca tcgtgggaga cgagcaccag
     5341 ggagcctccc gcctgatcct gcaactgctg gagctgataa acggcatccg ggcagaccat
     5401 ctggacagtg taagggacga gtattgccgg ctggtgcgtc ccaagtccaa gtcgcgccgc
     5461 atcctgcggg atgatatccg cgccgtggaa ccctttttgc aacagcgcgc agcggagggg
     5521 aaatggaaga tagccgtgtt catggatgcg gagcggatga atgacgaggc cgccaacgct
     5581 ttcctgaaaa ccctggagga accgcccggc cagtgtctgc tcattttgtc cacctcccag
     5641 ccggatcagc ttctccagac aattatttcc cgctgcgtgc gcgtcaatct gctgcaatcc
     5701 ggagaattca ggctcactcc tgttcaggag gccctgctcc cgcactggct ggagacctgc
     5761 cgcaatctga ataatgacct ggccgctctg gccttccgca gcaggttcat ggacgtcatg
     5821 gcagaggcca aggccgccat caccaaaaac ctgaatcagg ctctgaaaga tgaagccagg
     5881 gaagcagccc agggtacgga cgcctccgac tgggaggccc gcaataagga cgcgaatgcc
     5941 gcccagatag agacggaata tctggaacag cgcaatcagg ccctggaact gctcatcagc
     6001 tggttcggcc aggcagccct gattgcctcc ggagccccgg aagtcgctcc gattcatccg
     6061 gaggtgcgca cactggccgc ccagatgccc gtgaacgaat tgctgaagcg catggaggcc
     6121 ctcaacaggc tccgtgatga tttgaatttc aatattcacg aagccctggc cctggatgtc
     6181 cacctgctgg cggccgtcgg atcctgctga cgaacaaccc cgcctgttac tgtcgggccg
     6241 cgcacggcat tggaaaaggc gcagaaggtc tctattcttc cctttccttc cggagcaggg
     6301 gcgccatgat ttcctcatag gcgcggaatt tctgctcata cgtcagccgc gccgccgccg
     6361 gactggtgga aggcatttga atgacggaaa gcgtttcccg aaggaacagt tcgggaaaat
     6421 accgtttaag gtatttatgg gaagcggtgc cgttgcagca gaccacgccg atgcccggga
     6481 agcgatccag cagggccgca atatcattgg gccgttcctg ggaga
//
LOCUS       DAWEMX010000135         6481 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4781_length_6481_cov_10.515873, whole genome shotgun sequence.
ACCESSION   DAWEMX010000135 DAWEMX010000000
VERSION     DAWEMX010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4781_length_6481_cov_10.515873"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1312 a         1794 c         1791 g         1584 t
ORIGIN      
        1 gctgacctcc gtttcccgca gcaggttcat gatctgggaa ctggttaccg caatgacgag
       61 ctggtctccg aactgcgggg catcctggtt ccaggcttcg ttttcctcaa aaagctggag
      121 ggctgcgcgc agcttggcca cggtaaggtt gctggcggat ttggtgccgc tgtccacgtg
      181 atccacggct atttgctggc tttccttgaa tggaacggcg gtcatgccgt cttctcccac
      241 gtagttcgta tctaggaagc cggagatcat cacgtcgtcc atgcggcggc ctgctgcggc
      301 ctggagacct tcaatcgtct tgctgacggg aaggtccatg tctcccaggc gggtggcgtc
      361 aaattcatcg tagccaatgg ctttggtgaa cgtgcgcggc cgcatggacc ggcgtgtggt
      421 gggagcctcg ttcaaaacgg tctgccccac ccgtgtggtt ttttccgtga agtccagaat
      481 gccgaattga tccagaaata ctaccttgcc gctcaagtcg cgcatgacgc tgacatactt
      541 gtccagccgg gaggcgtgct gctggagcag ggagccccat tttctggtgt attttacctg
      601 ataattatcg ctgatagtta ctgccatgat tgcaaatata atgatagttt gaatgcagga
      661 acggcaattt ggattatcgg gtccggaata accggagcgg tcctgccgca agcgggatgc
      721 ggctatgtcc cctgcgggat tgcgggccgg tcccatccag cgctaacgtg tatggactac
      781 aagacagaaa agcgcaccac ggcagaatcg tgatgcgctg tataaggaat gaaaacttcg
      841 ccttcccgct gttaaagaat aatcgcgtgc gtccgcgtag cgtgatccgg gccgaaaagg
      901 gccagcatgc ggcccgtttg ccctttttcc atgcggtagg agtaaaaacg tttggtatcc
      961 tccgccgtgt ccagcccgga atcgtgaatc tgttcttccg gtacccccgc ttcaatgagc
     1021 tgtgccttaa tggccgtagg aatgtcttcc tcataatgag gcgggcggat acagggggaa
     1081 atgacggcaa tcaggttgga cggttccacc ccaaggcatt ttttcatgga cttgatggtg
     1141 ttggaaacga tgttgccggc ggttcccgcc ttgcctgaat gaatcaggga acggtggcag
     1201 ttttccttgt cgtacaacca cacggcggcg cagtccgcca cgtaaatggc aagcacgcag
     1261 tcggaaactc cggagcaaat caggccgtcc acgccttcca caggataatt ggagccgatg
     1321 tcccccacca gggcaacttt cgtgccgtgc acctgttcgg cgcggcgcag gtctttccac
     1381 cgccagccca gggcttccac ttccgcctta tggtacggtt ccagccgttc cagcacggtt
     1441 tcgcggtctc cggtgacgtc tactcccgga attctctcaa tgaattggac gatttctccg
     1501 ggataggact ggataggctt caagaatgct ggcgtttcca taattgatgg atcaataaat
     1561 gtcgttgggg acatcttgtc gttttgtatt acctagtcga gcaaaatttg gcgtaaaatg
     1621 tcacggacgc cgcccggtga ctgagaaaag gtttttttcc gcctttccgg agtgatatga
     1681 gcaattcatg ctgcgcaggt attctttact ttccactttt tttgcgggaa tggccgccgt
     1741 gtttttttcc tttgctgcct ccttggccca ggaggcttct ccggatgccg ggcagctgct
     1801 gaaaagggtg cgccaggggg caactcttca ggaaaacaag gacattaagg ggcaaatacg
     1861 caaacgcagc gtcaaaattc ccttttccat gagtctgcgc ggcaatctga tagccttcca
     1921 gtaccagctc aataatgtct ggaacaggtt cgacctgaaa ttcaaggacc gcggccagga
     1981 gatcctttcc tggaaggacg ggaaagccgg tgttttgccg gttgcccagt atgcggtgcc
     2041 cattgcgggg acggacgtaa cgtacgaaga tctttccatg cgctacctgt actggcccca
     2101 ggcaaaaatt gtccgggatg atgcggcgag caccgtcaag gggcgggatt gctggattgt
     2161 ccagataccg aatcctaatt ccggggtggg gcaatatgcc tgggtgcgcg tctggattga
     2221 taaggagaac ggcgccatgt ggcaggtgga tggcattgac cgccgcgggg agctggctaa
     2281 aaggttcatg attgattccg tgatgaaatt gaaggacgga tcctggtttt tcaaacggat
     2341 gaaggtggag gtccgggatc cttccaatcc gcgcagaacg gtctccgtca gctacataga
     2401 tatggacagc ccggaatagc cttccggttc ccgttccatt ccccgcggag ctttcggatt
     2461 attctttccg gggcatgggg gcggattcct tttcatgccg cacggatgga tgatttagga
     2521 cgtgacaaga aggcgggatg ggcgtaaatc ctccaaaaac ctgttatgaa gaaacattta
     2581 ttccttgcat gtgccctggc gttcggcctg tccgcctgtt cccagcacca ggagcagccg
     2641 gcggagtccg gttctccggt gaaaacgacc atttctcccg tgactgctcc tgggaaaaag
     2701 ccggaccggc cggccgtgaa attgcctccc aagtccgttc cggtgccttc cgtaagcccg
     2761 tcctataata atactcctct ttccccgcgc attccggggg taacggtcaa ccgtgtggct
     2821 gtgccggaca aggtggtggc cctcaccttt gatgacggtc cccatggaac gctgaccccc
     2881 agggtgctgg acatcctgcg cgacaataac gtcaagggta ctttcttcat gcagggctgc
     2941 aatgtgaagg cccatcccca ggtagtgcgc cgcatggtca atgagggcca tgaagtaggc
     3001 aaccatacat ggaatcacgt ttatctgagc aaagtgcccc gcgaaaaagc ggaatcccag
     3061 ctccagagca ctaatgaagc catccgcaac gcctgcggcg ttgtccccgt ggtcatgcgc
     3121 ccccccggcg gctatacgaa tgcaggcgtg gcgtcctggg cccgccagcg cttcggcttc
     3181 accacggtca tgtgggatgt ggacacgaat gactggcgca agcccggctc ctccgtggtg
     3241 gccgcccgcg ccgtgaacgg cgccaaaccc ggctccatca ttctggtgca tgacattcac
     3301 gcttctacgg tggcggcggt ggacgccatt gtgaagggat tgaaaaaccg cggctatgaa
     3361 ctggtgaccg tttccgaact tatcaggaga ggacgtgccg ccgccggcca tgctccttcc
     3421 atgcccgcgg ctcctgctgt cccggattct tctccccttg tcgttcctgc gattgtggct
     3481 cccgcggagc ctgttcccgc tccggcggag acggtggccg gcatgtaatc cccggtgttt
     3541 ttccttttcc tttcaacccg gtttccctgc ggaaaccggg ttattcattg cctttccgga
     3601 gcatttttcc caccgccccg gaaaggaggc tttcccctta acgggcacac gcacagaagg
     3661 cttgacctgc tggtgaattt ctccacaatc ctttgccgta ttattcacac ccaatccgga
     3721 ttgaaatgtc tatgaatctc ctggcatctg ccgatactct acccgcacct ggcggaatgt
     3781 ccgctcctct cgcttttatc tacgatctct tcaataaccc tcttttcatc ttcattggcg
     3841 gtctggtgtt gctgggtgtg tttttctggt atctgggttc cgaccaggac aaggtcaagc
     3901 gcaatgccgg aacgattttc attatcggca ttgcgtcttt ttccctgttc tccctcttcc
     3961 agcaggggtt gaaatacggc attgacattg ccgggggcgt ctccttcacg ttgagcgtgg
     4021 aaccgaatat cggagacgac ggcaagccca ttcccctgac ggaccaggcc atggaacagg
     4081 catgcgtgat cctgacggac cgccttaaca gtaccggggc caatgacgtc attatccgcc
     4141 cgaagaaaaa agacaacctg aatcttatag aagttcaaat ccccgctgcg gatcctgcca
     4201 agcgcgagga aaccaaggcc atcctgacca aagtagccaa acttcagatt ctccctgtcc
     4261 acccgcgtaa caacgaactg gtggcggagg ggcgcatccg cgttcccggc tacaggatgt
     4321 atgaatatac cttcaaaaac ggcaagggtg aggacgtcac ggaaaaaata ttcctggaaa
     4381 agcgtgaatc gctgacaggc agggatatcg tgcgcgccgg agtggatctg gcccgtcccg
     4441 gccacgtcaa cgtcaccctg agcaacgaag gggcggacaa gatgtataac ctgacttccc
     4501 gcatgcagct gggccacgac cgcatggcca ttgtgctgga tgatgtggtc aagagcgctc
     4561 caacggtcca ggccattctt tcgaaaagtt ttgaaatcag cgggctggat gctcccggag
     4621 aggctgaagc cctgacgaag gtgctttcca acccccttac caacaagctc aattttgagt
     4681 ccgccagtga aatttccgcc tctttggggc atacggccct ccttcagggg gaatacgctg
     4741 gcattacggg attgatcctc tgcttcatca tgatgatcat ctactaccgc tttgccggtt
     4801 tggtggctat catggggctg acgatcaatg ccctgcttct tctgggagcc atgtctatct
     4861 tcgggtttga gctgacgctg ccgggtattg ccggtattgt gctgactctg ggcgtggcgg
     4921 tggactcgaa cgtgttgatt tatgaacgcc tgcgggaaga aaaggaaatg gggcgcccct
     4981 tccgcgtggc tatccgcaac tcctttgaca aggcgttctc cgccatcttt gactccaata
     5041 tcacctcgct cattaccgcc gtcatcctgt actggatggc aaccggcacc atcaaggggt
     5101 ttgccgtcac gttgacagta ggcgtcatca cttccatgat tggggcgatt ctcgttaccc
     5161 gcgtcctgtt ctactgggcg gataccgtcg gcatgatgaa ggatcttaaa ttcctcaacc
     5221 tcttcaagaa aaagacgaac atcgacttca tgagccagaa aatctggtcc tgctctcttt
     5281 ccgccattct gctgatgata tgcattgtat ttggcgccgt aaagggcaag gattgcctgg
     5341 gcatggactt tacgggcggt tcttccattt cctatctggt gaacaagggt gacatctcct
     5401 tccgggaagt ggaaggcgtg gtcaacaagc tgtccctgac ccagaaggct acggtgcagg
     5461 aggtggcgga gtccacggac agcagcaaag tgaatattct gattaatttc tccgaccatg
     5521 cccaggacaa ggaggctatc accacggcat tggacaaggc gtttccggtt ctggacaacg
     5581 ctccgttcag tgaagaaacc atcgggcagt ccatggggta cgatacgctc attacttccg
     5641 catgggccct tttcttcggc attctgggaa tcatggttta cctgacggtg cggtttgaat
     5701 ggaccttcgc cattggtgcg gtcatcgccc tgacgcatga cgtcctgctg gtgcttggcc
     5761 tggtgattgt cagcggaacg gagctgaatg ttattcatat tggcgcgttg ttgacggtgg
     5821 cggggtactc catcaatgac aaaattatcg tatttgaccg catccgcgaa ttcctccgct
     5881 tctccgaccc gaatgaaagc gcttcacaaa tcatgaacga agccattaac cagacgcttt
     5941 cccgtactat tttgacctct ctttccacgc tggccgtgct ggtgtgcctg tacttctttg
     6001 gaggtccttc catggaagac ttcgcctgga cgatttccgc aggcattttg attggtacgt
     6061 actcctccat cttcatcgct tctccggcgg ctcttttctt ctcccggaag catgggttgc
     6121 atgaagaagt cagacaggcc atgaaggccg gagcctgagg tttggcgttg agccgttaaa
     6181 tctctgaccg gggaaggctg aatgccttcc ccggtctgtt tcttggaaag agagggaaag
     6241 gctctgtatg gaataacctc cgtgttcttt tcccgtccgc ctcgttcgct tttcctgttt
     6301 ccaccatatg cggctacggc ttccgcatgg ctcttctgtc tcgcattcct ccctctttta
     6361 tttcaggcat aagaaaaccc cgcctgccgt taggcagacg gggccgtatc tctggtttcc
     6421 tgctatgtat ttctgcgggt gagattattc atcatcccct tcctcctgtg ccggggcggg
     6481 a
//
LOCUS       DAWEMX010000136         6459 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4794_length_6459_cov_10.566365, whole genome shotgun sequence.
ACCESSION   DAWEMX010000136 DAWEMX010000000
VERSION     DAWEMX010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_6459_cov_10.566365"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5380..5389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1289 a         1726 c         1975 g         1459 t
ORIGIN      
        1 gattactgcg gcaatgccga tgatgatgcc caggatggtg agggctgcgc gcatgggatt
       61 gcgcaccagg gccctgatgc aggttttaag gagaggaagg aatttcatac gtcgtccttg
      121 ctaagcatat cggaaatccc ttcgcagata aggccgtccg ccatgttgat ggccctgtcc
      181 gtgaaggcgg ctattttggg gtcgtgcgtc accaggatga ctgtgattcc ctgttggcgg
      241 ttcaggtcct tgaacatgtg cagcacttcc ttggaagtgg tggagtccag gtttcccgtt
      301 ggttcgtccg ccagcaggat gctggggttg ttgatgaggg aacgggcaat ggctacgcgc
      361 tgctgctgtc cgccggaaag ctgggccggg gtgtggtcca tgcggtcgga aaggcctacc
      421 aaattcagca attccacgga acgttcgcgc atggctttca tgctgagggc ggggtgggca
      481 tatacggcag gcatcatcac gttttccagg gctgtggtac gggagagcag gttaaagttc
      541 tggaagacga atccgatgcg cttgttccgg agagtggcgc gttctgcggc gttgaatttg
      601 gcaacgtctt ccccggcaat atagtaatgg cctgaggtag ggcggtccag acagcccagg
      661 atattcatca gggttgattt ccctgagccg gatgcccccg tgagggagac gaattcccct
      721 tcattgatat ccagggtgat gcccttgagt acttgaaggt ctatttctcc caagtggtac
      781 accttggtta tgtcacgtaa gcggatcacg ttgttggaat gcttgatgcg gtaaaaaaga
      841 gtgattgagc ggtcggatga aggcggttcc ccgggttagg gaggcggggg cggcccgtca
      901 ttgccagccg aggctgccgt tttcgtgttg cctgtgctcg gcttgcggcg cgggggcatt
      961 ttgggcgcga acgggttttc cccgccggcg gaggcggaag aaccgtcccc cgaactgtcg
     1021 gagcggttca gtatctgagc cgtagagacg atttccattc cttcccggag cgtttctccc
     1081 gcaatggccg tcagcatgcc gttgctttcc cctttggtga ccaggtgggg ataaagaaga
     1141 ttgttccgca attcccaaac cacggctttt ttcttttgtc cgtcccggac gggggcggcc
     1201 agctccggct gcatccggtc aaaggccgct ttttgttcag cactgaccag ttctgtttcc
     1261 gggcggaagc ggagggccgc attggaaacg aggaaagcgt ttcgtatgtc ctccaccacg
     1321 aattggacat ttgccgtcag ataggggata agaagcttgt ccggattggg tacatcaatg
     1381 tccacgatgt aagtgaccac attggacgtc atggttgcgt ccagccgtat cttgtccacc
     1441 gttcccttga atttgcggcc ggcgaaagcg tccacggtga acagtacctc ctgccccttg
     1501 cggatgctgc cgatgtccgc ctcgttcaca gcggcccaaa ttttcagctt ggaaaggtct
     1561 gtggcgatgt aaaacaggct ggaggcgctc atgctggaaa ccactgtttg gcctatgttg
     1621 accaggcgtt tgaccacgac gccgtccacg ggggacttga tggtggtgta ttccaggttg
     1681 cgcatttcct ttttcagcgc ggcttccgcc tgtttcaggg aggcttccgc ttcctgcagg
     1741 gaggcttccg ccacggctac gttggcgcgg gcggtttctt catccgcaat atactggtcg
     1801 tagctggatt tggacagggc atccccaggc cccagctttt ccgcgcgttc ccggtccagt
     1861 ctggcctgct ggtgcttggc tttggcctgc tggatgtccg ccttggagcg ggcgattccg
     1921 gcaatggcct gcgctttgga ggcttccgcc ctctgaacgt ccagctgcac gggaagcttg
     1981 tcgatttccg cgagcatctg accggctttt acagggctgg aataatccac caccttgccg
     2041 ttcaggtcct tgccgaactc catgatgatt ccgctgacct gggcgcctac atctaccagt
     2101 tcatcaggct ccgtgacgcc ggttgcttcg atggtgatca tcaggtttcc tttttcaaga
     2161 ggttctgtct ggtagtccac ctggatggct tcatttcctt tccactgttc ccaggcaaac
     2221 cagatgcctc cgagaacggc aatgacgatg atgagtttga tgaatccctt cacgactata
     2281 ttatacttcg caataatgaa aaagttgcac ggatgttaaa aacttttccg caacggagca
     2341 tgatcctatc atgatttccg gaaatgtcta agggattatt atgtttatta acagttgctg
     2401 tttattaact aataatattt cctttcttcc ttctcgtcca gtgacggaag gagcgggaat
     2461 ggagggccac gaaatatctg aaggcggcag ttgccggtgg ccgcattcga tcttttgggg
     2521 aattggcttc tccgtatttg taaatgctgc gagggtgaga agagcgggga aaggaggtga
     2581 atcccgacag gagtaatgtt gcccaagagg gcaatcccgg ctgacctgcg ggctcaagga
     2641 ggggaaaaac gttgaaagaa cgagctttct ggattcaggt gatggaaaaa agacaccgct
     2701 gcaccgtctc tggctgtgag ccacgttccc gtgcctcaat agagcgggga cttccgccgc
     2761 tgttttgact tttccggtat aggacgtagt cagccctgcg tcggggctgc ccccattagg
     2821 ttgtaatatc ggtccgggcc actttgccag gcgatgacga gtgcagcagc caccacctta
     2881 tatacctcat ggtagggaat gttccaaggt gtttttgcaa tcttttcaga catggaggga
     2941 actttcccgt ggagcgattg attttgaaag ggatgctgct ttcagcggtg ttcttccagc
     3001 cattccttta acacaaggcg gtccgcaggt gcccagggca gttcttccag cgtacggcgg
     3061 gagaagaacc ccaggttttc atgttccaga ggccggggca gggcgcaaag ttccagacgg
     3121 cacagaaagg ggataaggcg gataatgcgt tcaggctccc ggtgccgtac gggggtgagc
     3181 atgcggacgg ggaagacggt gcatcccagc tcttcccgta tttcccgtat gacggcgtgc
     3241 cgggcattct cccctggttc cacttttccg ccggggaatt cgtaaagaag cccgttggac
     3301 tgccccgttt tctttttggc gcccagaaga agagggcccc gtgcggcggg cagttcaatc
     3361 agggcacagc agacgtccag cgtgttcatg gggcgtaaaa gtccaggtta tgcaggttga
     3421 tgatgcgggt gtccgtatcc aggacgccga aaccgatgcg tccggtcctg ccctggagtt
     3481 ctccgggatt ggccagcagg cagccgccgc atctttcgct cacggcctgg tgggtatggc
     3541 cgtacagggc ggcgtctgcg tttccgctgc gcgcttcccg caaggcgtat tttgggtaat
     3601 gggagagaga aagtttcatg ccgtaccggg tgataagggc atgttccgga tgcaggcgcg
     3661 ccgccgggga actggcggcc atccgccgca tggtttccac atcgtagtcg ttgttgcccg
     3721 gaacggcatc cagaggaaag tcctccgtca gttcaatcag gcgccggaaa ctctccgggg
     3781 tggtgcagtc gcccaggaaa atgaaatgtc cgcagcccag caggcgcatc cggtgcatgg
     3841 ctggctccag atggtctgtg cggttgtgca agtcggaaaa aatgcctatc ttcatctttt
     3901 ttcaacaatt catggcatga atgggccatg ccttcaattc atccggcagg gaaagcggga
     3961 gcggatggcc tccctccttg cgcagcaggc aggtgaaacc gcccgctcct gctccgtgga
     4021 agggcatgag ccggtagcag gcttcctgcg tcagggagga acggaaatgc tccagctccg
     4081 gcacggttat ggtgtgcagg tccggatggc gtttcagcag gtacaggaca ttgcgttcat
     4141 tttcctccgg agcgtaggtg catgtagtgt acagcaggaa gcctccggga gccagacacc
     4201 ggagggcggc cagcaaaatg ccccgctgcc gcttggcgtt gcctccggtg acggaggaat
     4261 tgaaacagcc ggggttggga atgcctttgg ccagcaggga ctgcccgctg cacggggcat
     4321 ccgccagaat gaggtcaaaa cacccggggg ccagttccgc ccactggtcc gggcgcaggc
     4381 gctgggtgta cagattggtg aagccgcagc gaagcaggtt gtggcgcaga atgcccaggc
     4441 gcttcggatt cacttcgttg gaaacatgct cctgcggaga tacccgcgtc tgcgccagaa
     4501 tgcttttccc tcccggcgcc gcgcacatgt ccaggcagcg ttccggacga aacggaagat
     4561 gcgccagagg ggccgtttcc cagacggacg aaagatccag gggatagtaa tagccgcatt
     4621 cgtaatccgg cagggagccg ggtttggttt ccgcctctcc ggacgggagc gccaggatgg
     4681 aggaatgccc ccactcctgc ggcgtgtcct ttgccggcaa gggcggcctg tagccttccg
     4741 gtgccttcgg ggtgatgacc agcgcgcttc tggaacggtt cccggcctgc atggcgtcca
     4801 ggaaatcccg ttcctgttcc gccgtcaatt ccagtttgcg ggcgagtttg agtatttggg
     4861 aggaagacac gagatcaggc ccggaatgtt tctccgaagt gggtgttgag agccagatgg
     4921 gcggccccca ggatgcccgc ctccgtaccg aactgggcgg gaaggatttc aaggtattcc
     4981 accagagggg cggcgagctg ggttttcatg atttcctgaa ggggctggaa aagcagggtt
     5041 ctggccttgg ccacgccccc gccgatgatg atggcttccg ggttcaggag atagcagcaa
     5101 ttcatcaggg cgcaggagag ttttacggcc aggtcccgcc atacttgctc ggctacttca
     5161 tcaccagcca atgcggcccg ttccaaagag atggggttgc agtccacaat ggccttgtca
     5221 atgccgtggc tggcatacag cgtgcgtgca tccgcagcta tttcccggtt gcctacataa
     5281 tcttccaggg agccgcggtt tccgtaatgg cccagacgcc cccggtagtc gatgctcgtc
     5341 tgccccagct ccccggcgga gcaggtagcc ccgcgcagan nnnnnnnnna agttctccgt
     5401 tcacgatcag gccgctgcca acgccggtgc cgagggtcag gcagaccaga tgcctttttc
     5461 cttttccggc gcccagcttc cattccgcat aagccatgca gttggcgtcg ttttccacgt
     5521 atacgggcag tccgcaggca gcctggagca tgtcctttac cggaacgttg ttccagccgg
     5581 gcacgttggt aagcgtataa acggtcccct ggtaaaaatt gacgaacccc ggcattccca
     5641 tgccgatggc ctgcacttcc gggtgattca gccgcagcat attcacgaac tgggctatgg
     5701 cttcaatcag ttgatcggga ttgccatatt cctgggtggc tattgaagga gcgtgggcga
     5761 ttacttccgc gcccttgacg acccccatct tgatggaggt gcctccgaag tcaatgccga
     5821 tggagggaat ggttgaagct gtcatataac gccaatatag aattttaccg ggcgttgaaa
     5881 aggttaaaag aagtatgttc ccggtaaaat cccgacagga aaatagaggg tgcaaaaggg
     5941 gggggaatga cttgtttcat cactgaattt tcttgaatga acatttgggc gtttgtaatg
     6001 tctcatagtc tagtcagcgg ggcgtgttcc gccctctgtt cgtgaaatat tgtcaacctc
     6061 agcccataga gacacctcct atgcctactc ccaagaaaac caatagcaag accgtcgcca
     6121 aggaagttcc ggcttccaag aaaaagacct gcgggaagtc cgattgcaag accgctgtag
     6181 acctggaggc tcccgctaag aagaaatgct gcaggaaaaa ggcggctgaa ccggaaaaag
     6241 ccgccaagcc cgccaaaaag gctgccgccg ccgcggctga aatggaaaag aagcctgcgc
     6301 cgaaaacggc cggaaaggct ccggttaaaa aagctgctcc tgctcccgtt gccccggttc
     6361 ccgctcccgc gaaaaatgaa ttgacggatg aacaggcgga agccattgcc gccgccaggg
     6421 cggaactggc cgccaacccc gaatgggagc cttttgtgc
//
LOCUS       DAWEMX010000137         6336 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4874_length_6336_cov_13.341028, whole genome shotgun sequence.
ACCESSION   DAWEMX010000137 DAWEMX010000000
VERSION     DAWEMX010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4874_length_6336_cov_13.341028"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1629..1638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1186 a         1694 c         1974 g         1472 t
ORIGIN      
        1 gtttcctttt ctggtctgcc gtaggcgggg tccagcctgt agctttgctc cagcgtggtg
       61 gtgggcatcc atccccggtt gctttgccct ggaagttgcg gatagtccag gtagcagtgt
      121 tccccggcag caatgatcag ggagagcccc tgctttttcg tctccgtgat ggtggacgga
      181 gtcagtccca tgcgccaggt gaagacggtg tctcctttgt tgtagatgtt ggccttggcc
      241 cacggttttt cataccatag ttggatgcgc ttgccgtgac cagccagcat ggaggacatt
      301 tttttgatga attccttcaa ttcctcgtgg acgtccttca tgcctttttg ctcccggtac
      361 tggatgctga gggggcattt ggcccaggtt ttttccaggg gtgcttcatc gccgcccaga
      421 tggacgatgc cggacgggaa aatatctttg agttccttga aaacagcggc ataaaatttc
      481 cagacttccg gtttgtgcgg gcagatgagg tggagggtga cgccttcatc ggtcttcact
      541 ttcgtatccg ggttcgggaa gcagaaaaat tccgggtagg ctgccgccag ggcctgattg
      601 tgccccggca cgtctatttc agggatgacc tcaatgccgc gcgctgcgca gtacgccacc
      661 agctctttca gttcctgttt ggtgtacatc ccttcatggg gaattccgtt gcgcatgctt
      721 tctttccgtt tggaggagat gcttttgagt ttggggtagc cgggcaccgg aagacgccac
      781 ccctggtcgt ccgtcaagtg gatctggagc ttgttgaatt tgtagtaggc catcatatcc
      841 acaaattttt tcagatcggc cgcggggatg aaaaagcgcg cgggatctac catgaggccg
      901 cgccacggat agcgcggctt gtccgtgatg acgccgcagg gaatgccctc cggctgtccc
      961 gccagctggt cccggagctg gcggagggtt gccagtccgt aaattctgcc ttcccttcct
     1021 ccggaagcaa ggtgcgcccc ctgcggggtg actgtgagcc ggtaggcgtc cgttcccagg
     1081 gtcggaactt cctggtcaga aagaagtttc aaggttctgg tactgttgtg ctgcagctcc
     1141 gttttttccg gttcagggat aacgctgtat ttgtccgctg cggcggcaca tgccatgtgg
     1201 gcggaaaaaa tggcgcaggc caggatgagg ggcagtttaa acatcatgaa gaactgtttc
     1261 ggaaaagatt ctaccatggt aagggatgcg tgataaggaa aaacggtgaa attacttgtt
     1321 gttttcccag cggaaggccg gttcattccc ctgagtgcgc tccatatcat aattgaagcg
     1381 tttgaggatg aattgacggt ggtcggccag cttgcgccgg aagttttccc aggagcgcac
     1441 gcccatcggt gaccagccgg cttccgcaat ggcgcaggcg cggggatagg cgcggtaaag
     1501 caagtggttc aagtccggca ggcgttccgc ccatacgggg gcatgcacgc cccggatatg
     1561 gcctgcctct ttctccggtt ttccaaaagc gggatccagg tcataacatt tttgcagggt
     1621 ggtggtttnn nnnnnnnngg taaatgccgg cgttcccctc gtaccagaat tgcggcgttt
     1681 gcccgttttt ggcaagcaga gctgccgttt tcgcaaaaaa ggctttcatc tgttcctgtt
     1741 tgtctttcat ggctgccctg gtccgggctt cccggcacag agggcatttt tcccagagtt
     1801 ccgtgggggc ctcgtccccg cccagatgaa cgataccgga cgggaagata tccttgagtt
     1861 cattaaagac ggcggcataa aatttccaga cttccggctt ctggggacag accagttcct
     1921 tgctgtttcc cgctgtcgtc cgcacgttca tgtccggttt ggggaagcag aaaaattccg
     1981 ggtaggcggc atgaagcgcc tggttgtggc ccggcatgtc tatttcaggg atgacctcaa
     2041 tgccgcgcgc tgcgcagtac gccaccagtt ccttcagttc ctgtttggtg tacatccctt
     2101 cgtgggggat tccgtctccg aagctttcct cccgcctgga tgcgacgctt ttcaatttgg
     2161 ggtagccggg cacgggcagc ctccagccct ggttgtccgt cagatgcagg tgcagcctgt
     2221 tgaatttgta gtaggccatc atatccacaa attttttcag atcggccgcg gggatgaaaa
     2281 agcgcgcggg atctaccatg aggccgcgcc acggatagcg cggcttgtcc gtgatgacgc
     2341 cgcagggaat gccctccggc tgtcccgcca gctggtcccg gagctggcgg agggttgcca
     2401 gtccgtaaat tctgccttcc cttcctccgg aagcaaggtg cgccccctgc ggggtgactg
     2461 tgagccggta ggcgtccgtt cccagggacg gagcctcctg gtcggaaaga agctgtaaag
     2521 ttttagctgt ttcctgcctg aattccgttc tggacggctc agggataatg ctgtattggg
     2581 ctgttgcttc tgccggagga gagaaggaga gcagaatgcc gccgagaatg ggtaagggac
     2641 gtgccatatt tccaatataa acggggagga aacaggttct gtcaaataat catcttttgc
     2701 ttccagattt tttgccgtct ccttgttgtc cggcgcggaa tcggtaatag aaagaaaata
     2761 aaaagaccgc ctccggagag acggcctttt tattagaagt ggtagcccta tgaggactcg
     2821 aaccccaaaa tacggaacca gaatccgctg tgttaccgat tacactatag ggctatgtaa
     2881 cgggcgcttt tgcggcgcgg gcgcattaaa gcggaagagg ccgttttttg caagtttttt
     2941 tttactcaaa gatatgtttt gattggaaaa aggctgtaca ggtaagcggc aggctgtttt
     3001 ttccggaatt atccggccag gcttttaaag tacgcctgta cgtgctcctc cgtgatttgc
     3061 tggcggtgcg cctgttcatg cctgccctgg gactgcttcc atggggagga cggggcggtg
     3121 ttgatgcgtc cgagctgctg ggccgtatat ttgccataga cgttccagat atgctccaga
     3181 aagggaataa tgtccgtttc ctcctccaaa gggggctggc ggcggctggg atgctctatg
     3241 gaagagtagg gcggctgatc cttgtattca tgataaatgg aaggaagtac cggaccggaa
     3301 ggccaggctt ccacccgttc attgatcagg aaagcgccag tccggtgcag atgccaccag
     3361 tccgccagaa tgaccagatg gttcagtttg acgggcgtca ggatgagccc cttggcgttc
     3421 gcctgctgaa tgaaccagtt gcagagtttt tgcgtggtag tggagtttgc catgctgaaa
     3481 tgggttgcct ccattataaa aggccgcagg tatttctcaa cactaaatcc gcttgcggtt
     3541 ccggcgctct ggcggtcggc ataggcaaat ggctgtgccg ggattcgtca ggtccccggc
     3601 atgccgcttc ctgctccgca ggcgggatgc ctgaaaatat ggcgcaggtt gttctagcgg
     3661 ggagggtgcc ggaggttccc ctctcccggg cagctcctgg tgatgtgctt ccccttccgg
     3721 agatttccgg gtcaggccgt gtgcatggca gtggagttgc tttccgggga agagaagact
     3781 gtcaaaggga aagaaagcgc gctctgcgga caacgtcttc cggagaaagg gcgtaaaggc
     3841 tgtccagcaa tgatacggca aaggaaccgg gggaggggct ttgttcaaag gccatttctc
     3901 cggcaatgcc catgagtacg gctgtggaaa cggccgcctg caggggggtg ggggagacgg
     3961 cggcgcaggc ggcggacagg gctcccatgg aacagcccac gcccgtaacc cgggacatca
     4021 tggcgtggcc gttttccgtg gcggttacct ggcggccgtc cgtggaataa tccgtacgcc
     4081 ccgtaacgag cacggccgcc cccgtgtgca gggccagttc cctagccgca tgcagggcgt
     4141 ctgcgctgga gctggtgctt tccggccctt tcgtgacgga ggaatagccg gccagggcca
     4201 tgatttcaga agcgtttccg cggatgatgc ggggggattg ttccttcaat tgccccgcca
     4261 gccgcgtgcg cagggaaaga gcccctacgg ccacaggatc cagcacccag gggatattca
     4321 gccggttggc ttcccgcacc gccgtttgca tggcggctcc ctgttcacgc gtcacggtgc
     4381 ccaggttgac cagcagggcg cctgttccgc tgcggagcat gtccaccgct tcttccgcgt
     4441 cgttgagcat ggcggggacg gcgcctatgg ccagcaggag attggccgtc aggggctgga
     4501 cgacggaatt ggtcagggaa agaaccagcg gggccgtttc cctgattttt tccaaatcgg
     4561 cggtaacggc ttgaacaagg tctgtcgatg aaagcatggc gttaaaaagt gcttgattgt
     4621 ccagtgtttc ctgcggtccg tccgtcaggc aagagcccgt gcggcggctt ccggattctc
     4681 cgctccgcaa atggcggaga caacggcaat tccggaggcg gttcccgtgg cgcgcacttg
     4741 acgggcccgt tccgcgtcca gtccgccgat ggcgacaacg ggcaggggag attgggaagc
     4801 cagagcggcg aagccttcca ctcccagcac gggaggcgcg ttcagcttgg aaatggtggg
     4861 aaagacgggg cccatgccca ggtagtccac caaggaggca tccacggcgc ggagctgttc
     4921 cttattggag acggacagtc ctaggatttt atccgggccg atcatggctc tgacggatgg
     4981 aaccggaaga tccctctggc cgatgtggac gccgtctgct tccagcgcca gagccaggtc
     5041 aatgcggttg ttgacgatga agggaatacc gcgggaagcc aggaaatccc gtatgggcaa
     5101 agcttcccgg taacaggttc ccgcatccgg attggtggag cggtactgca cgatggtaac
     5161 tcctccgtcc gccgctttcc gtacggtttc cagaaggctg aggcggcatt tggatgcttc
     5221 atccgtgact aggtagagat ggagattgaa ttttttcatg gcagggaatg gaaggaatca
     5281 gtccgtaaac agaggggtgg aaagatagcg gtcccccgtg tccggcagca ggacgacgat
     5341 ggtctttcct tcattctccg ggcgccgcgc cacctgtgtg gcggcccaga gagctgctcc
     5401 ggaggaaata cccgtcagca cgccgtccgt gcgcgccagt tccttccctg tggagaaagc
     5461 gtcttcattg gtgacggcga tgacttcgtc gtacacggag gtgtccagcg tttccggcac
     5521 gaagcccgcg ccaatgccct gaatcttgtg ggggccggcc gtgccctgtg tcagcacggg
     5581 agaggagaac ggctccacag ccacgatccg gatgtccgga ttacgggatt taaggtactt
     5641 gcccacgcct gtgatggtgc ctccggtacc cacgcccgcc acaaaaatat ccgctttgcc
     5701 ttttgtatca ttccagattt ccgggcctgt ggtgcggaaa tgcgcttccg ggttggacgg
     5761 gttggcgaac tggccgggga taaagctgtt gggcagtccc gcagccagtt cctccgcctt
     5821 ggcgatggct cccttcatgc ccagcgcgcc gtccgtcagc accagttccg cgccgtaggc
     5881 tttcaggagg ttgcggcgtt ccacgctcat cgtctccggc atggtcaata taatccggta
     5941 cccgcgtgaa gcggccaccg ccgccaggcc gatgcccgtg tttccgctgg tgggctcaat
     6001 gatgacggaa tccggcttca gcttcccggc ggcttccgcc gcatcaatca tggcctgggc
     6061 aatgcggtct ttcacgctgc cggcagggtt caggtattcc agcttggcca ggatcgtggc
     6121 gttcaggcca ttcctgcgtt cgtaattggt cagttccagc aacggggtgc cgccgatcag
     6181 ttccgtaatg tttttataaa ttctcatggc ttgtaggaaa agagcgttgc tgccgttaaa
     6241 tcgttagaga atggcgcgga agccttgttc cagatcttcc agaatatcgt cgatatgttc
     6301 cgttccgatt gaaagacgga tcgtattctg ccggat
//
LOCUS       DAWEMX010000138         6321 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4891_length_6321_cov_13.578519, whole genome shotgun sequence.
ACCESSION   DAWEMX010000138 DAWEMX010000000
VERSION     DAWEMX010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_6321_cov_13.578519"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1376 a         1651 c         1872 g         1422 t
ORIGIN      
        1 gtgtcttggt ccgctgcttc ttcaattccg caagggcatt ttcagtactt tcaatgtgga
       61 gactcataga gggaggtgtc tggttggcat caaggcaaaa actcccgcat ttctaaaaaa
      121 tggggagaat tgccctgtac aggtatgtat tgctggcctg cctgtttaca aggctggcag
      181 catactaaag ccagacacgc ctgtctgtca atcagattag gaattcctgg tgccttcccc
      241 cgtgccggga aggaagcggt aatacacctc cagcatcagc gtagcgaggc actggcggta
      301 aattttgtcg ttgatggaac ctccattgct gttcacgagc ccccccggtc ccgggaatcc
      361 gttcggggtg aagcttccat cggtggcctg ggctccgagg gtggaatcac ggaaggcctt
      421 gttgtacgct ttccatacat cccctccgcg gttcatggcg gcctgtaccc cgtaatagtg
      481 ctggtagagg ttggaggaag tattgttcgc cttccagtta aacggctggt tggtgttctt
      541 aagcatccag tccagaccct tgcgggcggc gctgtccgaa ccgttgccgc acatttgaag
      601 ggaaagaacg cctacgccca ccagggaggg acgtgctgcg gcgtcttccc tgccttcctg
      661 catgtaggtg aagaggccgt cgggcttgcc gcatttccgg caataggccg cggctttgcg
      721 cagggccgtg cgtatttcgc ctttggaggg gcggatgcag cgatgttcgg cggccttgag
      781 cgcctgtacg ttccatacgt tgacggaaag gtcattatga gcacccgcct tcgtgttgta
      841 attgtaggcc cagccgccgt cggtattttg attgtccaca atcttatcaa cggcctttat
      901 gacgactttt tccagattgg agatagaggg gatgtccatc tgcttgcaga aggtgtaggc
      961 ttcgcacagg gcataggtgg ctaccgcgtg gtcatagctg acttcgttcc tctggggaac
     1021 cagggagaga acgggatttt tcatgccctg atccaccagg aaagagatgc ccttcaggac
     1081 agtttccccg aattcttcgg aaagaggcgt ttcgcagtga gccaggtagg agaggagagc
     1141 catgcctgtc atggcgcatt tgtaattatg gggagccccg ccccaggaac cgtctttatt
     1201 ctgctgtttt ttcagccagc ggagggattt gactacggct tcctcacact gcggggtgcc
     1261 cccgttttcc gtaatgcgct tcatgcggtc gttcaaatcg cagcgcttgg agatgatgcc
     1321 gggaatgcct cccccgtctc cgtcgcccgt gccggtaccg aagccgtcgc cgatgtcgat
     1381 gcccatggag agttcttccg aggggacgtc cacctcaata tccgggttga ccaccgcgac
     1441 gtccgccgtg gcgacggcgg cgatgacctt cacctgcgcg gaggtggagg aactgctggg
     1501 gcgctgggtg gtctgggtga cttccggtgt gtcgatgtcc gggttgtcgc cttcctgggt
     1561 ggcgtaggcg accatggggg gatcttccgg aacggccacg atgatgtgca ccaggtagag
     1621 gaggaggccc atgagggcga tgccgaggaa ggagacggaa atggcggcgg tcagtgtctt
     1681 ggtccgctgc ttcttcaatt ccgcaagggc attttcagta ctttcaatat gcagactcat
     1741 acaatatgtg aagcttcctg aggatagtag aatgtttttc cggttgaggc gagtccaaaa
     1801 attttgatgg tctatagggt ggtaaatgac cagatatgaa caaaatggaa ttttataatg
     1861 aacggcttat attgattgcg tcatatatcc gatgggagtc cagacatttc cgtagcggga
     1921 gcgcctgccg ggacacgggc tgtgttcctg gtttgtcgga ggggaatgaa aaaggctttt
     1981 tgaagccggg aaggagaaac aacacgccgg gaacagtttg cctttgagtg ttttttagct
     2041 gtggccggca gaggaagaga gggcggttcc gggagaggag cgtacgaaga aatttgaaaa
     2101 aacaagccct gccatggagg gcatggcagg gcggaaaaga gggtggactt ttgcgggtag
     2161 aggcattaat agtggccgcg gcgcgggccg gtcatccact tccatgcggt ttccacaatg
     2221 gccccggcat ccttgcacct ggcttcccag ccgaggattt ccttagcttt cttcgggttg
     2281 gcaatcaggc ggggcgggtc gccttcacgg cgcgggccgt agcggacggg aatttttttg
     2341 ccggtgactt tttcagccgt ttccaggatt tcccggacgg aaagtcccag gccggttccg
     2401 aggttgaagc attctgtggc cccacctttc atcaggtagt ccaccgcttt taagtgggcg
     2461 tccgccaggt cgttgacgtg gatgtagtcg cggatgcagg tgccgtccgg cgtgtcgtaa
     2521 tccgttccga agacttcgat gtattccttt tccccggtga gcgtgagcag gatgttgggg
     2581 ataaggtggg tttctggttc gtggtcttca ccaatcaagc cgtcctcgct gcaacctgag
     2641 gcattaaaat agcgcagaaa aacgcttttg agaccccagg cgcggtcgca gtccctgcaa
     2701 actttttcca gcatgtactt ggaactcccg taaggattga taggatcctg cttttccgtt
     2761 tccgggatag ggatctgggt gggaacgcca tacgtggcgc aggtggagga gaaaacaaag
     2821 cgcttgacgc cggcggcttg catggcgccc agaaggacga ggggcctggc gatgttgttc
     2881 atgtagtact tgagggggtt ctgaacggat tcccccacat tgatgaaggc ggcaaagtga
     2941 atcacggcgt caaagttgcc cttcatcagg agaggatata ccacggaggc atctcccaaa
     3001 tcccctttga ccagtttcac ttccgggtcc acgagggctc ccgcgtggcc gtacaccatg
     3061 ttgtccagca cggtcacgtc tgcgccggtt ttgacgagct ggcgcaccgt gtgggaacca
     3121 atatatccgg ctccgccggt tacaagtact ttcatgattt tatcaaattt gctgttgagc
     3181 cggtttaact ttttctccgg caaaggcgtc cggacatttt ggagtctgcg gaaccttccg
     3241 gttcactgca ggctgaatcc ctgttccaga cgcctgtaaa agtgacatgt tttccccttt
     3301 ttgtcaattt gcgatatatc ttgccgccgt ctctccgttc tttgcctgcg gcggttggag
     3361 cttctccgtt ttcatgccgg aaactgcccc gctttttact ggctcctgac aggcattttt
     3421 gcgaacatca cttcattatg aaaccgtccg agatcaggaa tgttctggaa gaccatgaag
     3481 tgcgccccag caagtccctg gggcagaatt tcctgacgga tgaaaatgtg gcccgctgga
     3541 ttgtggagca gctcgaaatc cggccgcagg attgcgtggt ggaggtaggg cctggaaccg
     3601 gctccctgac ggaacatgcc gcaccccttt gccgcaaact ggttctggtg gaatttgatt
     3661 cccgtctggc ggagtaccag aaagagcgct gggccggtga tccccatgtg gaggttcacc
     3721 atgcagacgg cgcttcctgg gatccgcggg ggctgtttgc ggaggcccct gtcaaattcc
     3781 ttggcaatct tccctattcc gcggggggag ccatcctcca gaatttcctg tccaggccca
     3841 gtgctgtgga acgtgccgtg gtgatgctcc agaaggagtt tatcgaccgc attctggcga
     3901 cgccggatga tgatgccttc gggctgttgt ccctgcgcat ccagaaaaac tggattcccc
     3961 gtgcccttaa aaccattccg ccggaagcgt ttcatccccg tccgcgtatt gattccaccg
     4021 tgatgctgct tactccccgc cctgcccgcg agctgcctcc atatgacgac cgcctgatgg
     4081 atgaactgat gcgcaaggct ttttcccagc ggcgcaaaca attgaaaaaa cagctccccg
     4141 cttctcctcc gtgggagggc gtggctgctt ccctgggact ttccccttcc gccagggctg
     4201 aggaattgaa tttgtcccag tgggtggaat tggcgcgcgt ttatgatacc aatcctctca
     4261 aggatgtggc gcagagcgga gatgaattgt ttgacatcgt ggacgaactc aatcaggtga
     4321 cggggcaggg cacgcgccgg gaaatacatg aaggcagcct tcgtcatcgc gccgtacata
     4381 tgttcctggt caataagcat ggagctgtgc ttctccagaa aaggtccctg tggaaagaca
     4441 ggcagccggg caaatgggat tcctccgcag ccgggcatct ggatgccggg gaaagttatg
     4501 aggaagctac ggtgcgggag ctgaaggaag aactgggggt gtccggctgc gggctgcaga
     4561 aaatcgcgga ttttgacgcc ggagaaaata acggctggga attcatctcc ctctatgaag
     4621 ggcggtattc cgggaaggtt cgttttccgg cggcggaggt ggacagcgtg cagtggttta
     4681 cgccggagca aatccaggcg tgggtggaac gccgtccgca ggatttctcg cctgcgttca
     4741 ttccctgctg gaatgcatgg ctggccgcga atagaaaaaa tcattctaac tcaaatacat
     4801 atcatgataa accctgatca attatctccg gaacagatcc agtccatccg gacctgggcg
     4861 gaagaaggac tggatttaaa cgccatccag aaaaggctgc atgaggactt gaacatcaag
     4921 ctgacgttca tggatacaag attccttctt caggatctgg atatccgcat cagacagccg
     4981 gagcctgaac ccgaagccga gcagcccgga gagcttcccg gaacaatgcc tccccctgcc
     5041 tccctgctgg gaaaaaccca ggtaactgtg gatgaaataa cgcctcccca ctccctgatg
     5101 gccggcaagg ttttattcaa gagcggtgcc cagggcgcat gggatataga ccgtaccggc
     5161 cgtatcaact gggacgctac gcttggagaa cctacggcgg acgacctgag ggaatttgaa
     5221 acggaactgc aatccgtcat tcgcagccgc atgggagcca tgtgagggaa acggaaaata
     5281 ttccgcttga aatggactgg aagcaggaac ttgccgggtg gcggttattt ccaataaaag
     5341 ccctggctgg aatgggaatg ccgcgtccgc atgccattcg tgagagaatg cctgcccgga
     5401 aaaaatgcgc gttcatttgg ttccatagta aaataaaaac aacaaaagga ctatcatgga
     5461 aataccggag gaaatgattg aaacgctggt gcctgccgtt cgggagcagc tggagtcgcc
     5521 ggacaccgct tatgtcaaaa actgtctgga acgcctgacg gggcgggaaa aactgggaga
     5581 acaggaagct ctggaactga tggcgcaggc tctggccatt actattaatg cgatggtggt
     5641 ggggggccgc tcctttgatt cgggaaagta caaggcgttg ttgaaagatc tcccggctct
     5701 tccggatgag gctccgtgat aaatctcgac aatctccgga attgattcat aatcctttct
     5761 ttcacttgaa attttatggc aaaaaaacca ttagaaacag gtgctccgga agcgtccgca
     5821 gctaaaaaaa cggctgctcg caagacgacg gccaaaaaga cggcctctac taccaaaaag
     5881 gccgccgcag ctgaaaaaaa agctgctgct gacgccgctt ccgtagaagc acccaaaacc
     5941 aaaatcaaaa cggtcaagtc cgccccgaaa accgcggtga agacgaaaac cgcgaaaaca
     6001 gccaaaaagg cggctgatga aaaggcccct gtctctgaaa cggttgttgc cccttccgag
     6061 gccccggctc cttccgctcc cgtggaaaag gagatgatgc ccgaagctcc ccagaagccc
     6121 caggctgccg ctccggtcaa ggtcaagcgg gaatcgttcc gccccaatcg caatgcggaa
     6181 cagcaggagc gccatgaaca gcatggcgga gatgtctttg cccagccgga aacggtgggc
     6241 ggcgcctccg aagggccctt taacaagcgc aagcgtcgcc gccgcaataa gaaaggcggc
     6301 ggttccgaag accgcccgcc c
//
LOCUS       DAWEMX010000139         6316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4895_length_6316_cov_11.656924, whole genome shotgun sequence.
ACCESSION   DAWEMX010000139 DAWEMX010000000
VERSION     DAWEMX010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4895_length_6316_cov_11.656924"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1308 a         1984 c         1720 g         1304 t
ORIGIN      
        1 gttcatccct taaaagggat tcaggcagat catgcgcttc tgcgtccagt gcccgtaggt
       61 ggaagaatac acatccgtaa tttcccgggc ccattcgtca atagttcgct gatgatgggc
      121 gtatttttta aaatccttgg tgtccacgcc cagcagggag ctgtggtctt cctcataatc
      181 cgcaaattcg gagacgatgc ggccggactc atccctcagg cgtcccgcca tggcaatgtg
      241 ccctttcttg atgaaccgct tgaccagccc tccccccttg atgccgctca gcgccgtgac
      301 aaagatgccg gccctggcat tcgtgggagt gatggaaatg atggcgagtt ccagcgtata
      361 ggcccccctg gttcccggag gcaccatttc aaaatgcgga ttttcggcaa cctccttttc
      421 catgctggtt gcaacgctct tctggaagta ttccgccagg ctgcgcaggg catcaaaccc
      481 ttccgcggta tccggcctca catccatgtg caccgtatcc accggaacga cggccatcag
      541 gacttttttc ccattcacgc cgttcacccg gtcattccag atttttttat catcggcatt
      601 ccagtacgcg tcaaagggaa tatgggaatc cttatttttt acctgatgag tgataaatcc
      661 tgtttgggga acatgtttga aattattgga acaagacact accgccatgc agccaagcag
      721 cacgagggcc atcagggaag ccgggaataa aaaacggtgt ttcttcatat caagtaacag
      781 gcagaatcgt ttttatcatg gcctggcacc agctgtcaat aaaacctgcg ggtgaaaatc
      841 ccgggctggg gctctcctgc cgggcagaaa gagccaaggc gggaattcct agtcttcttc
      901 atccatgggg cggtatttca cggccagttt ttccaggatt ttcagggccc tggaggcgcg
      961 gctgcggaag gatttgtagt cccgggggtc gcagtcgctc caggcgcgtt cggacagggc
     1021 cagggcgcgc ggccatgcca tgtattccac cagccccata ttgtacaggt attccgtcca
     1081 gatgttagcc tgaaggccgc gcaccctggc ggcttcctcc ggggagagtt ccggcaattc
     1141 atagccgtac acctgcttga gcggaataag gcccccgaca gctttcggct ccgtggatgc
     1201 ggcagactgg taatagtcaa aatagaagtg ggaacacggc gccagaatga cgggatgccc
     1261 tttcctggct gccgccgcgg caaagccgtt gccgcgccac gccataacga ggatgtgttt
     1321 gtccgcctcc agttccccgg cttcatccca gacgatggcg tcatagccgt ctttccgcac
     1381 gtagtccgca atctgctgca ggaaatcccg gcccagggcc ttgaggtcct tctccctcag
     1441 gttctttaaa cggttctggc aataccggca ttttttccat gcctcggcag acacctcgtc
     1501 atggcccaaa tggatggggg agccgggcgg aaaaacgtcc ttgagttcat ccaccacgtc
     1561 tttcacgaag cggagcgttt ccgggcgtcc catgcagacg gcatcccgtc cttttttccc
     1621 ctccacggga aaatccacgc cgtcgcaaat caaatccccg taggcatgca tggccgcata
     1681 ggaatggccg ggaaggtcta tttccggaac aacggtaacc tggcggttgc gcgcataggc
     1741 aataatctcc ttcatctgct cctgggtgta aaaaccgccg taggtagctt ccgggtcatg
     1801 ctcctggatg gcgagtttca cttcctgcca gttccactct ccgtcagcga gcggaacgcg
     1861 ccaggcgctc atggaggtaa gcaacggata ttttttaatt tccagacgcc agccgggacc
     1921 gtccgtcagg tgcaggtgga ggcggttaag cttgaacagg gacatagtgt caaccatgcg
     1981 cttgacttcc gccatggtaa agaagtggcg ggaaacatcc agcatcaggc cccgccattc
     2041 aaagcgggga acatcccgga gggaaaaaac gggtacggaa aacccctccc cggtccggtt
     2101 ggcgcttaaa acctggacca tgctctgcca cgcgtagaaa aggccctggg gcgccgccgc
     2161 tttcagatgc aggatgcccc tcccttcccc gtcgcgctcc aggcgggcct catatccttc
     2221 tcctgtcccc gaggggcttc cctcaaccga acagacggca tccgcagcgg ttttgtcatg
     2281 ggttatggag aggccggaca aaacgccctg ggcattgagc acattcagga gggcttccac
     2341 tacaggaaca tccttttccg gagcatagac cgccaaccgc aacgggagat gcgccacggc
     2401 ggcccggtca gacctgcatg cggaaggcgc ggggagcagc acctcctcgg ccggcagcat
     2461 ttccgccatg ccggcgcccg gtaatccgga cagggccagc catgctgcca gacggaaaca
     2521 acacccctta ttcatcatcc cggaagtacg gcccaccctg gtctcttctt tcaatggaag
     2581 gagctttccc tcagtcccct gtgtcccgga aggcaaaggc gcggccatat tcagccgcgc
     2641 ctgccggatt ccgtcctttt ttacattcac ctgcgtccgc caaaaaggcc gccaaggccg
     2701 ccccccaatc cacccaggcc cttcaggccg cccaaacctc cgcccatgcc gggaagtccg
     2761 ggaatcttgc cccccttccc tttcatggaa gccatctgct tcatcatggc gcccattttg
     2821 cccttgccgg acatcatctt gcgcatttcg gaaaaattct tgatcagctt gttcacctcc
     2881 atcagaggac ggccgcagcc cgcggcaata cggcggcggc gggacggatt caacaggttg
     2941 ggattgctcc tttcctttgc cgtcatggag agcacaatgg cttccatgtg cttcatccgc
     3001 ttggggtcca gggcaccttc cggaagctgt ttcttgattt tgccgaagcc ggggagcagg
     3061 cccagcagtc cttccagcgg tcccaggttc tgcatcatct tcatctggtc cagaaagtcg
     3121 ttgaaatcaa atttgccgga catcatgcgg tccatggatt tggcagcctg ctcttcgtca
     3181 atgcgggcgg cggcatgctc caccagcccg acaatgtcgc ccatgcccag aatgcgatcc
     3241 gccatgcgac cggggacgaa ggggccgaac tgatccagct tttccccctc accggaaaat
     3301 ttgatgggct tgcccgttac ggaacgcatg gaaagagccg cgcccccgcg ggcatcgccg
     3361 tccagcttgg tcaagacgat gcccgtcacg ttcacggcac ggtcaaaggt ctgggccaca
     3421 ttcacggcct gctgtcccgt ggcggcatcc gccaccagga gcgtttccct cggcgaaagg
     3481 aaggcgtgca gctggcgcag ctcgtctatc agggcttcat ccacttcctg acggcccgcc
     3541 gtgtcaaaaa tcatgacggt tccgttctgg gtccgggccc attccagagc ctcgcgcgcc
     3601 acggccacca cgtccctgct gcctggtgcg ggcttataaa cgggcactcc tatttgttcc
     3661 gccagcgtcg ccagctggtc cacggcggcg ggacgcacca ggtcgcaggc caccagcagg
     3721 gggcggcgcc cctcgttctt caggcggagg gccagcttcg cgctggtcgt ggtcttgccg
     3781 gcgccgttca gccccaccac catgattctg gcagggtccg tcagatccag ccccacggag
     3841 tccccgccca gcagggcggc gatttcatca cggaaaattt tgacgatttg ctctccgggc
     3901 ttgatggatt tgagcacttc ctgccccatc gcccgttcct tcacgcggcc gatgaattcc
     3961 cgggccacgc caaactccac gtccgcctcc agcaaagcca gccggatgtc gcgcagggcg
     4021 tctgcaatat ttttctcgga aattttgccc aggccgcgca gcctccggaa cgtgttatcc
     4081 agtttatctg ctaaaagtga aaacataatc gtatgatgcg ccagactgtg ccacatttcc
     4141 cccttcccgt caacaagctc gccctgcatg ggacatgatg cttgacttgc cgggggcttc
     4201 gccccatctt ttcccgcatg gcaccctttg acctgaaaga ggaaatctta cgcctgaaag
     4261 aaaaacgcaa cgccatcatt ctggcgcaca gctaccagac cggggatatc caggacatgg
     4321 ccgactacgt gggcgattcc ctggggctgg cctacaaggc gcagaatacg gattgcgacg
     4381 tgatcgcctt ctgcggcgtc catttcatgg cggaaaccgc caaaattctt aatccggaca
     4441 aaaccgtcat ccttccggat gctgatgccg gctgttccct ggaagcctcc tgcgatgccg
     4501 gggaactgga agccttctta aaggaaaacg cccataaaaa ttactatgta gtggcgtatg
     4561 taaactgctc catcggcgtc aaggctctgg cggacgtaat cgtcacctcc gggaatgccg
     4621 taaaaatcgt ctcccaggct ccggaggacc gccccatcct gttcgtcccg gaccagaatc
     4681 tgggtgcatg ggtaagccgc caacttggcc gccccctgga actgtggaac ggggcctgcc
     4741 acgtgcacat ggagttcacg cgggaccaga ttcttgccct gaaggaaaaa caccccggcg
     4801 tgccggtagt ggcccatccg gaatgcacgg aagccgtgcg cctgctggcg gaccatatct
     4861 gctccacgga aaaaatgatc ccctattgca cggaaaaccc cgccaaatct ttcatcattg
     4921 tgacggaatc cggcattctg caccgtctcc gcaagctggt gccgggaaaa gaattcattc
     4981 ccgcgcccac ggagcaatgc tcctgcagca cctgcccgta catgaaaatg aacacgctgg
     5041 aaaaactgta cctagccctt cgggaccttt ctcccgccgt ggaactgccg gaagacctgc
     5101 gcagaagggc ggaagccccg ctgctgcgca tgctggagca atcccaatcc tgacctctgc
     5161 cttccccgca tgatacgcct ttcagaccac atcgcggccg ctggcggctg gctctccctg
     5221 gaagctttca tgcagctggc cctgcaccac ccgcagaaag ggtactactc ctcttccatt
     5281 gaaaacatcg gtcaacgcgg ggatttttcc accaccccca cgctttcccc catccttgca
     5341 aaggccattg tcgcgcactg gaaagaagcc tgctcccgct gcggcaggcg gctgcctctg
     5401 ctggaaatag gcgccggctc cggtgccctg gccgttaaaa tcctggagca gctgggattc
     5461 tggaaccgcc tgaatacgga ttacgtgatt gtggaatctt ccccacgtct gcgcgaattc
     5521 cagcaccttc tgctgggagg ccgcgctaaa atttactcca ccatggaaaa agcgctgaaa
     5581 cactgcggag gcaaggcctt tattttctcc aacgagctgg tggatgcctt cccggcgcgc
     5641 gtatttgaat acacggaaca ggactggaaa gaagtggggc ttgtcgtgaa aaacggagcc
     5701 gtccgggaag aactgcgcgc cgtccggcag cagccgcttt tctcccatat gctggaatac
     5761 ggctcccagc cggggcagcg ggtggaaatt cacgactcct acgcgcgctg gtttacgagc
     5821 tggcttcccc tctggaacat gggcgtcatg acggtcatcg actacgggga tgaaatggag
     5881 cggctgtact atcgccgccc ccggggttcc ctgcgcgggt acaaaagcca ccaggtgctg
     5941 acgggggagg aactgtaccg taaccccggc ctcacggatt tgacctgtga cgtcaatttt
     6001 acggacctgc tggaactatc ccgcaactgt ctgggggacc gggtcacttt catgacccag
     6061 cgggactacc tgctccccca tgcggaaaac acgccgcagg atgcctttct aacggatgaa
     6121 tacggtgccg gaggacactt ccacgtactc attcaggaac gccagcggct gcaaccggaa
     6181 ggcacccagt aatccaggag accgtacttg gaaatgccgg acgaaaccta agcaagcccc
     6241 cgtccgacag cgacggcccc atctttctcc gagagaaaaa cattgattac aggaattaga
     6301 ctgatgcccg gaaggg
//
LOCUS       DAWEMX010000140         6215 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_4978_length_6215_cov_9.863961, whole genome shotgun sequence.
ACCESSION   DAWEMX010000140 DAWEMX010000000
VERSION     DAWEMX010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4978_length_6215_cov_9.863961"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1327..1336
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2125..2134
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5943..5952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1656 a         1198 c         1511 g         1820 t
ORIGIN      
        1 gattatcttt tcctggatgg taagcaattt attgattatt cttttgcggg aataacgagg
       61 atagcctgat acattgtgca aatggaaaca gattacgtcg gcgtcggaaa agacccggga
      121 gggaggcgtt tccgtcttat gctcaaaaag ataggcatac tgctccagct gttttttggg
      181 ttccagatga ttaaggaaat atgcatggca tcgcttgcca aaagcggcta ttgttttgtt
      241 ttcagcaagg gtttcaatga ctctgaaata caggtcaatt ttattgtcgg ggatcagaaa
      301 gccgtttttc ccgttttcta tcaaatccgg aatagctgat cttttcaaaa gattgcatat
      361 agggatggtt ccggtggaaa gagcttccac aaccagcatg gggccggtat ccaaaagtgt
      421 cgggataatg atagcatccg ctttttcaag aagttgcaga aattcatccc gcggaatatt
      481 gccacggaaa ctgatgcgtg gatcctgaag acgttcttgc cacgtttcag aaacgctaag
      541 gcaccagttc aaatgaaatt tcaaaggaga atggataagt ttttccgcca gggagcgcat
      601 gatgaaagct cccttgtttt tactttgccc acaggcacat ataagattga tggaattctg
      661 agttttctct ttgtgttcgg aaaagaaagg agattgattc cagttggtct ggtgaatgca
      721 aacgaattta tccgaaggaa tccggtattt ggccgccgct tccctgcaca tggtctggtt
      781 ttggcaaacg atatagtcga catatggcca gttacagcac gcgattttac cttgtatgcc
      841 aaagttggtt agagtttttc catgataaaa tccatgcatc acggatattt tgacaattga
      901 ctggtctaaa accggccata agccggacag ataggaaaca tcattgttga tgataatatc
      961 aggggcaaga gtccggatgg aatccaggag agcaatgaca gctgattttt cctgccgata
     1021 ttcggcagga atatggaaaa aggaatagtt ataatttgtt ttgtgtggga atacttcctt
     1081 tccttccctg accaaataga cggtgacgtg atgtccggcg gaaacaaggc ctgtagcaag
     1141 atcatggaca taagaggaaa ttcctccccc ctggcctaca gaggcaagtc ctgtcgtagt
     1201 aatcagaata ttgtagctgg ttttcatgct gcttgtcatt agtctattat tgtgaggccg
     1261 atgcttcgag atattgctct ttgggcttca taggttttgt atgagaacat tccatttgac
     1321 tgatatnnnn nnnnnngata tcttacttgg ggaaaatgct ttaatatgct cacgggcgtt
     1381 ttccgctagg atgcccaagt cgtccgggtt ggcagcaaga gaaataagtt tgttcgcgta
     1441 ttcctgttca ttgaatggct ggatcagttc ccccgttttt ccgggcagga taatgtctct
     1501 ggctgccggg taactgtcaa aagccagggg gacgcaaccg tgttgcatgg cctccgcaag
     1561 aacaagccca aagccttcaa aggtggaagt caggcaaaaa agcgaggatc gcgcataata
     1621 gggttcaggc tcctgaaatc cttcaaaaga aatatttttg aggttcagct tatgtgccat
     1681 ttctttcaac atgccggcat cctggccgtt gcctacaatg tccagatgcc atccgggaaa
     1741 tttttcttca aaaattttcc aaatacggag caggcggtct attcctttcg ctgcgttatc
     1801 aagcctgccg acgaataagc agcggcattc cttttggcag ggcggggtat tctgtgctaa
     1861 atcgggaatg acattgggaa tagatgttaa tttacaggaa ggagtctttg aataaatttt
     1921 ttggaattct tcaattcctc ctgaagaaag ggtgatgatc cggtcgtagt tgagagtaag
     1981 atcacggaat agttttttct gcaatatgca ggcccgtaac ctctttatat tcaaataaat
     2041 attgttatag atgaaatttt tcagtctgcc tgaaaaggaa ttttcaaaat gtttcttttt
     2101 aataaagcgg tagtatttca gatannnnnn nnnnaactat gttggactgc tacaaatttt
     2161 attttatttg attgtatctg ttttctgttt ttgaaaatgc catattttcc tatgatgcct
     2221 ccttccgtct ggtcaatgat gagttctgtt tgtttttggc ggataagatc gttgataaag
     2281 ttccttttct cttttttatt cagtttttgg gggatgaaaa taagagggac cggagggcga
     2341 taggggacat tcagcctgtt tttttgctga cagagcaaac tgacattgtg tccggaatct
     2401 cgtaaaaggc atgccaactg atgggtagcc ttttccatgc cgccctgttt gcccagaggt
     2461 tcgttaggta ggtaaataat aatgttactc attgccgttt ttcaggacat gaagccattt
     2521 tcgggttatt atttcttctt tgtaattgtc caatacttta aagttgggcc tcctgtagcg
     2581 ttctttcatc aggccgtcca gggtttttgt gtattgctta agattgaaag cagggacaag
     2641 tcttcctgtt tccgtatccg gaatcatttc ctttgcagca tggaaactgt caaaaaccac
     2701 aggaatgcat ccgtaggcca acgcttcatt aaccaccata ggcattcctt catgtgtgga
     2761 ggtgagacat acgatttttg attttttgta aaaaggttcg gggttctgat gtcccatgaa
     2821 aaaaattcgt ttcaaatgat attcttcgga tattttttcc aggttttctc tgtattctcc
     2881 gtcaccgaca atatagagac tccaatcggt atggacaggt tgaagttttt tccatacgct
     2941 caacagcctg tctactcttt tgtcagcata actgaggcgc cctacgtaaa gaagaatatt
     3001 ttgttttgta ccggaagata agctttcagg aatttggaat gtcagaaggt tggggatggc
     3061 atataatttg tgggcattat gggtggaggt aagtgaaagg cagtcctgaa tatatttttg
     3121 agaaagcacc accactttat ctgaatattc aagcaaatga tttagttttg ttttcttttt
     3181 gcgaatggcc atgtattttt tccatggagc cgatacaacg cgtgcaactg ttttcaattt
     3241 ggctagccat gtgttcccgg atgggggaat gagggacagg aaatgatcaa gtccttccca
     3301 gatggagaag ttaataatgg aaacgatggt ggcttttgtg ttcaaatggg cgtggctgaa
     3361 gaagaaggat gagtcggagg cggctccttc attcaaaata aaattgatat tttgttcatc
     3421 gcaaagtttt tctgtaaaat cggcattgcg tctgctgaac aagtattcgg aatccggcag
     3481 ggagtattgc gggaattcat tatttttttc tttgaaaggg tgaagagaaa gaaaaataat
     3541 acggaaccct tggcgaacaa gcagcctggc caggagaaag gaggcctgtt ccgtgcctcc
     3601 ctgaatggaa gtgatgggat aatcaaagta gatgagtata tttttcatgg ctatcatatg
     3661 aaagagcctt cccttccgga atgatcagac ggtattcccc tgtgaacgat taagggacaa
     3721 tctgtttgcg caaaatgttc agaggctgtt atttcgaggt ctttttcaaa gagacgtaca
     3781 agtccttgcg atttgatttt gccaggcatg cctgcgatga atggagcttt tacattttcc
     3841 agagaatagt cccgattgag cagggaatgg gatgctacta gcgaatatgg ggggagaacc
     3901 gttcccttgg caatataaga ccctgaactg atgaaagaac cctgaccaat ttttacggga
     3961 atggcgtatg gattaatatt tcctgtccgt atattgcgga aaggatggaa atccgtgtcg
     4021 taaatgcagc agtcatgggt aatggcgacg tagtcttcaa tgacgataga gcggtaagct
     4081 ctgatggaac cctgggcgcc gagactaaca taacggccaa attccagaaa ggactcaggc
     4141 ttgctggcgc caatgaccac attgacgctg ctgcggaaag ggcctttcca ccggatggta
     4201 ccggatagct gaagcaaagt gccggcatag ttagtgctga accggtcata atcatatccg
     4261 agacgaatga gtcccggttt cagttctgaa tcgggaatat caaggagcag agctccccct
     4321 tcccgtgcta ccgttaatga tccgtgtatg aaaaacggaa agtgtttggc ctgcctccat
     4381 ggtaaaaaac taatattgaa ccacagggtt ttgacgatat tcaacttccg ttttttttct
     4441 aaatataatc tgaagaaaaa acgatggatt tttctaataa aggatatttt agatagcaaa
     4501 caggccataa ttggtatttt ttatttgatg cagtccaaaa tcattccaat atggaggcgg
     4561 ggagaaatgt tccagagcgt tttgatgagg tggcgatccg taagcaaata agataaatag
     4621 agtgtgcgga agtgatggag aagtttgctg cggtaacctg acgacggaag attaagtact
     4681 gcaaacaggg atagaaggcg gcattccgtc cgcattcgtc ttgtggaagc ctttttctgt
     4741 aaatggagca tggtttctac aaacaagatt tcatcccctt gtcgaatgtc tgaaggaaac
     4801 tggcgcgaat tgatggcgga agtttctctt ttaatataat ggtaaactgt ttgtggagtc
     4861 atggctacga gatgactgcg catggcaagt tgagttactt gcagaagatc ttctccgatt
     4921 ttaagagaga gaggacaaag cagaggatgc tcaaacaatg attttctaaa caaaagcccc
     4981 cataggtggc cttgccatgt tttcctgatg gttgtcagcc attcttctcc ggaaagggta
     5041 gaaaaaggga tatagttcat tcgggcgatt agctctctct tttcagtcaa gtgaagagtg
     5101 gtaccggctg tgatgtcagc ctgggtttcc acagcccgtt ctaccaaaac ttccagggat
     5161 tgagccggta aatcatcgtc cgcatccatg aagaacaaat attgtccggt agcttttcgc
     5221 ataccgtaat tgcgtgcttc cgtgacaccg cccctgtttt gcctgaaggt gcgaatctgg
     5281 ggataggcat gatcaaaccc ctgaacaatt gcccgggaat tgtcggaaga gccatcatca
     5341 ataacgatga tttccaggtt tttgtaggtt tgccccaggc agctttgcag gcatgaatca
     5401 atggtttcct ctgcgttata caaaggaatg atgatggaaa caagaggcgg agaatctgga
     5461 gtcatgaaag gccgatttga gcaataattt gactggtata ttccgggtcg gaagatagtt
     5521 cagaaagacg atttcctgtt tcattgacat atctccacgg caccggaacc tttgacgttg
     5581 ctttcatttg aaaccactcg tattcgatgt cgatatgtcc tacgtcaata gattgtactc
     5641 cgtgttccgc taaatcatga gccaagaccg ttgctgttgg gcccagggcg attagaacca
     5701 gaaaatccat tccttttgct ttgtccagcg tcgtttccag aatagcatgg tagtagttaa
     5761 aagcgttttc cgccgggcag acgattcggc ggatggatgc ggctccttca aaaaggtcat
     5821 tgtaaaaacc cagtttagaa cctgtacctt ccaccagaat gaggtgacgg ttatggaaaa
     5881 tctggcgcca gaggctgatg tattgcggag tttccgtctt gtctttcaga tgttcgtaaa
     5941 atnnnnnnnn nngactggaa atatttgttg aaaagggtca gtccgtttcc ccggaataaa
     6001 aaatccttcc agaattttct ggattcttta ttgtaagatg agatgccgga aaaaacatca
     6061 gggatgcaga cccggtgttg cggtattggg ttccgggcta tttccctaag gcgcctggct
     6121 aatgccgcat cctctttttg aaatccaata ggatagcctt ccatgagaag tatttcccca
     6181 tccccatacc tgctgatgga tatgcggtgc ttaag
//
LOCUS       DAWEMX010000141         6171 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5019_length_6171_cov_15.612492, whole genome shotgun sequence.
ACCESSION   DAWEMX010000141 DAWEMX010000000
VERSION     DAWEMX010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_6171_cov_15.612492"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1314 a         1663 c         1860 g         1334 t
ORIGIN      
        1 tggtgcttgg cgggaggggc gccgaaaagg gtgcgccccg tctggaccag gtcccggcgg
       61 ttcagggaga agttcttgtc aatgaggaag tattcctgtt caggccccag cgtaatggtc
      121 acgtgttcat ccggaaggcc gaaactcttc atcagcttct tgacggcatt gcccaatgcc
      181 tggcgggagc gcagcagcgg agtcttctta tccagagcgt ctcccgtata agaacagtag
      241 gcggtaggga tgcaaagcgt ggctccgttc ccgtgcctct tgataaaggc ggggctggtg
      301 gggtcccatg cggtgtagcc gcgggcctca aaggtgcagc gcaaaccgcc ggaggggaag
      361 ctggaggcgt ccggttcgct cacaatcagg tttttgccgg aaaaactcat gatgggttcc
      421 attcccttgg gatccaggaa ggaatcgtgc ttctccgccg tggagcccgt catgggctgg
      481 aaccagtgcg tatagtgagt ggctccccgt tccagagccc actgcttcat ggcgtgggct
      541 acatccgcgg caatatccgc gttcagcgga agcccgtcct ggatagtggc caggagggtt
      601 tttgccgtgt ctttgggcag gtacttcttc atcacttccg tgctgaagac atccgctccg
      661 taataatcaa tgctgaccgg agcgctctcg tttgctccgc ccacgggcag ggaagcgatg
      721 gcgctgatgg cttgtgttcg tgtcgagtca ctcatcatgg ttaactgttg tgccttttct
      781 ctagcaagaa gcgtgccaac ttttcaggta aactttcgca tgaatgtgtc gttcatttta
      841 atatattgat tattagttaa atatgaatat catgacggaa gagctgcaaa aaaggaaaac
      901 aaaactgcga acgagaatgg attacaaaaa tgtaaaaccg caacaaaagg atgattacga
      961 aaatgtaaaa aattcttctt ggaagaaaaa tattttttat tccgctgcaa aatgtcctgt
     1021 tatgccggaa gccgcgtcag gctctgtaaa acagggacaa agcagtttga atgcgggaaa
     1081 atatgaagtc tggacaatgt tggcacggtt tttgctgcaa ataagtcagc ttaacccaaa
     1141 caaaacagca aactgatgaa tagctatcaa ttcgagacag gcattcctgc gccgcaggga
     1201 ttgtatgact ttgaacagga gagggacgcc tgcggcgtgg gtctggtggc tgacctgaag
     1261 aatgaaccga gccacaaaat cattgaaatg gggattactg tcctcaagag gctcatgcac
     1321 cgcggcgccg tggggagcga tccggatacc ggggacggcg caggcattct gctggccctt
     1381 ccggatgaat tcttccgcct ggttcttccc aacaaacttc ccgccagagg caaatacgga
     1441 gtggccatga tgtttggcgg atgcggccat gaagaggaat tggaagccgc cgttacggaa
     1501 aacggcggca gcgtgattgc gtggaggcag gttcccgtgg accgggacag catcggcaaa
     1561 aatgcccagc gcacgtgccc cctgatccgt cagctgttca ttgacggcag cggttttgcg
     1621 gaccaggcgg aatttgaacg caaactgttc gtgatgcgcc gggaaatgga acggcgcgtg
     1681 gaaggctgtt atgtgtgcag ctgttccagc cgaagcattg tgtacaaggg gctgtttctc
     1741 ggcacgcaga ttgaaggatt ttatggagac ctggccagcg agcatttcaa atctcccctg
     1801 gccctcgtcc accagcgcta cagcacgaat actttcccaa cctggagcct ggcccatccc
     1861 ttcaggtatc tggcccacaa cggggaaatc aacaccctgc gcgggaacct gaaccacctg
     1921 agcgtgcgtg agccgcattt gtcctccacc ctgttggggg atgacctgca aaagctgctg
     1981 cctcttattc cccccgggca gagcgattcc gcctgcctgg acaacatggt tgagctgctg
     2041 gccgcctctg ggcgggactt gcgccatgtc atgctcatgc tcatgccgca ggcctggggc
     2101 gtcaactacc atttagggcc ggatgtacgc ggcttctttg aataccactc cgccatgatg
     2161 gaaccctggg acggtccgac cgccgtcgtt ttttccgatg gtatcaatgc gggcgccatg
     2221 ctggaccgca acggcctgcg ccctgcgcgc tatacgctga ccacggatga tattttcatt
     2281 ctggcttcgg aaacgggcgt agctgatatt cccgcggaaa aagtagcccg caaaggccgc
     2341 ctccgtcccg gggaaatgat ttactgcgac ctggtgaacc accggctggt atccgatgct
     2401 gaaaccaaga atgaaatggc caggcgcatg ccctaccgcc gctgggtgga gaaaaacaaa
     2461 atttccgtcc gttccctgtt tgattccatt tccgcctctg cggaaatgcc ggacctcgtg
     2521 ggccgccagc gccagttcgg attcactcag gaggatgtgg agctgattat ccgccccatg
     2581 atgctgaaag gcgcggagcc tctcggctcc atgggaaatg acgcgccgct ggccgtcctc
     2641 tccggaaagg cgcctctcct gttcaattat ttcaagcagc tgttcgccca ggtgaccaat
     2701 ccgcccatcg accccatccg ggaggaactg gtcatgagcc tgactacata cataggcaac
     2761 catcccaaca ttctggaaga aacgccggag cacgcccggc tgatcaagat ggcgcgcccc
     2821 gtcattacgg atgaagaact caaccgcctc tgcaatattc gggaggccgg tttcccttcc
     2881 gccaggcttt ccatccagtt cccggaaggc ggggacggga aagccctgcg ggagacgctg
     2941 gaaagcctgg cggaatccgc cgtgggtctg gtcaggtccg gcgtgcgcat tctggtgctg
     3001 acggaccgca acatagggca cggctacctt cccgttccca gtcttttggc ctgttccgtg
     3061 gtgaaccgcg cgctggcggc cgccgggctg cgttccgacg tagggttgat tctggaaacc
     3121 ggggaggcga gggaaacaat gcactttgcg ctgcttctgg gcttcggggc cacggcggtc
     3181 aatccgtacc tggctctggc gacggttact tccctggccg caccgcagga ctgccctctg
     3241 gatgtagtga aagcctccgg aaactacatc aatgccatag acaagggact gcttaaaatc
     3301 atgtccaaga tgggcatttc cacgttgcgc agctaccgct cctcccaact gttcgaggcg
     3361 gtgggtctga accgggaact gattgacgaa ttctttcccg gcaccgtcag ccgcgtagga
     3421 ggcataggtc tggatgaaat agccgcggaa tgcaaccagc gcgccgcgca aaacgcggaa
     3481 cacggggaca agctggatgc gggagggcag tacaagtata agaaaggagg ggagaaccat
     3541 ctctggaatc cgcagacact tcaggctttc cgcgctgccg tgcgtgacaa tgacgaacgg
     3601 aaataccggg aattcgcgga ttacagcaac cgtcaggcgc agcatctctg cactttgcgc
     3661 ggcctgtttg aatttgctcc ggcggatgct atccccctgg aagaagtgga aagcgtggat
     3721 tccattttgc gccgtttcgt ttccggagcc atgtctttgg gttccctcag cccggaagcc
     3781 catgaaacca tcgccattgc gatgaataaa atcggagcca aaagcaattc cggggaaggg
     3841 ggggaagatg aagcccgcta tgaacccaac gcccggggcg aagtccgcta cagcgccatc
     3901 aagcaggtgg caagcgggcg ttttggcgtg accattaatt atctgcgcca tgcttccgag
     3961 ctccagatta agatggccca gggagccaag cccggggagg gggggcagct cccggcgcac
     4021 aaggtggatc catacatcgc ccggctgcgc cattccatgc cgaacgtcag cctcatctct
     4081 cctcctccgc atcatgacat ttactccatt gaagacctag cccagttaat ttacgatctg
     4141 cgcaactcca atccggatgc ccgtgtttcc gtcaagcttg tttccgaggt gggcatcggc
     4201 gcggttgccg cgggggtggt gaaggcccat gcggatgtgg ttgtggtcag cggccacgac
     4261 ggcggcacgg gcgcctctcc gctcaccagc gtgaagcacg ccgggctgcc gtgggagctg
     4321 gggctggcgg aaacgcagca gacactggtg ctcaacaaac tccgttcccg cgtccggctc
     4381 caggtggacg gccagttgcg tacggggagg gacgtcgtga tggccgccct gctgggggcg
     4441 gaggaattcg gtttcggaac cgccatcctg gtctccatcg gctgcgccat gctccgcagg
     4501 tgccatgaaa atacgtgccc ggtaggcgtc gccacgcagg atccggccct gagggccaaa
     4561 ttcagcggca agccggaata cgtcatcaat tacctccgct tcgtggccca ggaaacgcgg
     4621 gaaatcctgg cttcactggg gcttcgttcc ctagatgaag ccgtaggcag ggcggatctg
     4681 ctttccgtaa accgcgccat agatttttac aaggcccggc atttggactt ttcctccatc
     4741 ctgcatgcgg cagaggggga cggacgcagg tttgacccct cttttgaaaa ggaaccgctg
     4801 gacaactacg accgccgcca tctgctgccg gcattaaagc ccctgctgga aaagggggaa
     4861 aagacggaac tctgccgcga cgtccgcaat gtggaccgga cggtagggac ggaactttcc
     4921 ggtgagttgg tgatgaaatt cgggccgcgc ggcctggcgg aagatacgct tacggtgcac
     4981 ttcaccggat gtgcggggca aagcttcggg gcttttctgg ctcccggcgt tacctttgaa
     5041 cttgtcgggg aggcgaatga ctttgttggg aagggcctgt ccggcggcaa aatcatcgtg
     5101 cgtcccccgg atcatatctc ctacgtaccg tcggacaacg tcatcgccgg aaacgtcatc
     5161 ggctatgggg ccacttccgg cagcatcttc cttcacgggc aggccgggga acgtttcgcc
     5221 atccgcaaca gcggcgccaa aatggtggtg gaaggcattg gcgaccacgg ctgtgaatac
     5281 atgaccgggg gccgtgtggc ggttctcggc aaggtgggcg tcaatttcgc cgcggggatg
     5341 accggcggcc tggcctacgt gtatgatgcg gacaacaact tcgatcagaa atgcaacctg
     5401 ggcagcgtgg acctggaaac ggtcatgccg tattccgccg atgaagcgga attgcttcag
     5461 ctcatttacg agcattacat ggctaccagg agccgcaggg cttacgacct gttaaacaac
     5521 tggccggaag aacgcggcaa attcatcaag gtattcccgg tggaataccg gcatgccctg
     5581 gccatgcatt cctgaaagaa gcatgcagca gttttctgtt gtttggggta aggcaggggc
     5641 ctccgcgggt gcggaggccc ccattttttc aaaggcctgc ctgctgtttc atggccgggc
     5701 attttggcgc cgcttttcct ccttgagcca ttccgcattt gaagctggca atttgcctgt
     5761 ccggcggcat ttcggaggct gttccgttcg tttgccggat aacggccgtt ccggccgccg
     5821 ggcatttgga gacgaatgtt ccggataagg aggtttttat gaccgtgctg tcagttgata
     5881 aagaaatgcg gctgcatgga tggggatggt ggttgcggtt gatgtcagga ggaaggtcaa
     5941 atgttccggc aatgcccggt tctgagcgga ttatgaattc gaagtcaaca ggaaagtttg
     6001 aatggaaatg tgttagtgga gtttgggttt tggaaattcg gctggatggc aattgataaa
     6061 aaagtgaatt gaaaggtaac tgattggtat tcttgttatt tagttattca gggaattgcc
     6121 gcgaattcat aatccgttag gtttgctcca gtgggtgttt ggcattttct g
//
LOCUS       DAWEMX010000142         6092 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5110_length_6092_cov_11.019712, whole genome shotgun sequence.
ACCESSION   DAWEMX010000142 DAWEMX010000000
VERSION     DAWEMX010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5110_length_6092_cov_11.019712"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1484 a         1793 c         1424 g         1391 t
ORIGIN      
        1 gtcttctatc gtcagcacgc tgccttctat tctgtattgc cctgtatcta agcctagtac
       61 gctaaagccc tcgccaagct ccgctcccaa atcaacggta ttggaacctt cggcactaaa
      121 tccctgcaac aaacgcccaa tatctatttt cccggacgtc cacgtggtat ttgaagcagt
      181 aatcccatca tttcccgatg aagacgtata agataaaatt gctccatcaa ctgtcacatt
      241 ctgcaatgtc ccggtcccgc tcatgttcaa cgtaatggtt gatccgatcg tttgcgagtt
      301 aaaatcaagc gtccccccat ccagcgtaac aaggccgctt cccaaagcgg aattattcat
      361 gcaccttaca gtagagccgg aggtgatgac ccattcagca gtcatggatg cattggccgc
      421 attgtttaaa accaacgtac cgctgaatcc ttccccgccc accgtaatgg tccgtccgga
      481 agaattcagt gccccgtaaa cattcagcgt cttgccattt gccacaagga cattccagtc
      541 tgcattcaca ttaaccgggc cccatgcatt ggaggaagaa cccagggaaa aatcttcaca
      601 aaccgtaaat aataccggct cttccgtacc cgctctcccc agtataagag ttctctgggc
      661 attattgttc gggacaaggg agaaccctga tgccccttcc tccacaatca atccccccat
      721 ggtaaatggc gtaaaggagc aggatggatt tttattggtc gccgatacgg tgacatcgcc
      781 cacttccgtg cctgatgcga caaagcgcaa tgtgttatat tgcgacagtc ccgtttcatt
      841 ggcaaaaagg ttccaaatac tcccctcctc tgaaacggaa ccccaattcg tattgacagg
      901 accgtatgtt ccatccggat tggtcaggac aatgccgccg ttatgaaaat cattggacga
      961 actccctacc cacgtataga tattaccgga atatctgctg ccatcccact ccgtagcagc
     1021 gaacacatga aaaccggata tggcataaaa cgacaataag gcggacaaca acttgagcgg
     1081 tagacgtaac ttcataactt tcaaatatgt gtaaataata aagctatcca gccgtcctga
     1141 ttggctgttt gcaatatata gctattaccg aattagcaat ccgccgtcaa tattcaacat
     1201 tgatccaatg cctgttgcat acccggaaat catcatcttc ttaatatcca attcagaatt
     1261 ttttcaaaaa aacattccat atctctttca ttataaacat caaaaatatc tgattctggt
     1321 ctattttctg gataatgctt ctgcggattg ctaatccgca atgcaacatt tcacatgaaa
     1381 ataaaaatgg acgcaaaaat tatttttcat cccttcccca ttcctgaaca aacgatgcag
     1441 caaaataaca cctttcgtga atttcacctt tcatacatag aaaaagaggg tgtattgttt
     1501 gcccgcaaca agttcggagc aaagaccaga aatcgtagca gtagctgata cgtcccgttt
     1561 actgagcagc cccgtcccgg atgtccgcgt tccccttcac gaagataatt ctcttcgttc
     1621 ccatcggcac gctcagtccg gctcccagct gcagcgccgt cgttcccact ttcgcccccc
     1681 tcacgctttg cgcaaagccg gggttgccca gcagagagac gttcgtttcc cctctccggt
     1741 ctcccaggtc ctgcgccgcg tttacgcgga tttccgccag cgcttctcgt ccgaagatgt
     1801 tgctgcctac caggcccatc catcagtaag taaatatcgg aacagctggt ttgcggaaac
     1861 tccttcgcag gagataacgc caaatccagg caatcccatg aaaaacaaca ctcttcccat
     1921 aaaaaacgta ccgagagggg aaagacttcc aagacctgtc cggataaagg aaaagcaacc
     1981 gggaatgcac ctttcctcct tgctcccggc tcttcaagtt gctaaaatgc ttcaagcaaa
     2041 ttttttatcc ttttcccatg gccatttttg gttcctccaa tttatccgac tcaggtgatc
     2101 tgttgatctt caacaaagta tcttataaaa ccctactgat gttcgaaaaa gaactgggca
     2161 gggaccgcat gacttacctg gtggaagaag gtctcccccc ggatacggcc accgcctccc
     2221 atctggaatc cactaaagcg gacggcatcc tgttccaggc tggaggcagc gaccctgtcg
     2281 cgctgcgttc cgccatcatg gaacgcatta acgaaggcag aaaagtagta tttctacccg
     2341 gtcccgtctc ccatgtgaaa ggttccatca gccaaattcc tccgcgggtc atcaaggccc
     2401 tggaagccct gcatatctcc cccgtaccgg tctatgccgg tttttacacg aactccgtgc
     2461 tggatgcgga ggccgatacg gacgcccagg cggacatcca gatacatatt ctccccaaac
     2521 tggcccccgg ggccgagatg gcagcacgcc tgacctccgc gtggctggaa tgttccgccc
     2581 aggcatacgc cacgctgcct cagctccacg gttccctgtc cgccctcctt ttccgcagcc
     2641 tcagacttca ttccgattgc cgggtcatcg acggcattga cgacactacg ctgacttacg
     2701 gccagctact ggccatttcc gtagcctttg ccaagaggtt gaaaaaaatt acctcgaatc
     2761 gccgggtcgg catcattctt ccgccgggca agggagcggc catagccaat ctgggctgcc
     2821 tgttcgccgg gaaaacaccg gtgaatttca attattccgc ctcggaagga gcctttgcca
     2881 gctctgtaaa gcaatccggc gtggactggt ttattactgc ggataccttc atgcgaaagc
     2941 tccagaattt cccgtggccc ccccagcggg atctgatcct catggagcgg gaaattcccc
     3001 tgcttaaagg ttccgccaaa cgctggggcc tcgccatcaa attcctgaca gcggggttca
     3061 tgattaaaaa actggggctg gacgcaccta caggcgcgga cgaagccgtg ctgatgttca
     3121 cttccggctc ttccggggaa cccaagggcg tgccgctgac ccaccacaac cttctttcca
     3181 acatctctca atgttcctcc cgcattacgc tggaaccgca aagcaggttt ctgggaagcc
     3241 tgcccgtatt ccactgcttc ggcatcacca tcgggctatg gtatccgatg atcggcgggt
     3301 acgacatggt cacctatcca tcccctcttg aggccaaacg gctgggagcc cttatcaagc
     3361 agtacggaat cagtctggta gtcaccacgc ccaccttcct gcgcggtttc atgaaacgct
     3421 gcgaaccgga cacctttaaa accgtccgct acctgatcgt tggcgcggaa aaactgccgg
     3481 aagacctttc cgccgctttc cgggaaaaat tcggcatcat tccatgcgaa ggctacggcc
     3541 tgacggaagc ctctcccgtc tgttccgtca acttcattga cccggcgcca tccaatgccg
     3601 ccggagactt cattcccggc atgaaaaaga gttccgtagg agccctcctt cccggaatcg
     3661 ccatacgcat caccagccct cacacgggac gcgtggttcc catcactact tccggcatga
     3721 tctggctgaa aggaccgaac atctttcccg gctatctggg cggtccggag acggatcgcg
     3781 atattttcgt ggacggctgg ctaaaaaccg gagacatagg ttccgcagac gaattcggct
     3841 tcctgaagat tgaaggacgc atttcccgct tttccaaaat aggcggggaa atggtgcctc
     3901 atgaagccct ggaagcagcc attatgaaca tttggaatct ggatccggcg gacgaagaac
     3961 ggcggatagc cgtcgtcacc attccggacc ccgtaaaagg ggaggccgta gccctgctca
     4021 ccaccctggt gacggattac gtgcaccagg cgcgcaccct catcaggcac ggcctgattg
     4081 accaggggct gccggccctc tggtgcccca aggagattat tcccgtagaa cgcatcccgg
     4141 tgctcccatc cggcaagctg gatatcaagc aatgcaggat gctggcgtat gaagcgctga
     4201 acatcccctt tgaaccgtaa tttatggaat tcccggagga aacgcccgcc gcgcccaaac
     4261 ccatcacagt caaacagctc gtttaccgcc tgagagacac ggtcagcatc gctgtgggca
     4321 cccagtgggt ggtgggagaa ctgagcaacg tcaagcatca caccagcggc cacgtttatt
     4381 tcaccctgaa ggaacaggga gcggaaattt tctgcgcctt tttcaaggca gcggcttcca
     4441 aatgccccat acggctccag gaagggatga aagtccatgt cctgggcagc gccaccgtat
     4501 acccggaccg ggggcaactc cagctggtca tcaggcaggt aaaagctgca ggacagggag
     4561 atttgcagac tcgctttttg gagttgaaag ccaaactcca gcgcgagggg ttgtttgacg
     4621 cggaacacaa aaagaaaatt cctacgttcc cccgcgccat cggcatcgtc acctccccca
     4681 ccggcgccgt catccaggac atacggcacg tattggaacg ccgcgccccg tgggtgaagg
     4741 cttatctgct gcccgtacgc gtgcaggggg caggagcgga gcacgaaata gccgcagccg
     4801 tgcgcgcttg gtccggagcg cccttcaacg gtctcccgcc cgtggacgtg ctcattgtag
     4861 gacgcggagg cggctccatt gaggatttgt ggaatttcaa tgaggaaacg gtggcgcgcg
     4921 ccatttacga atgtaccgtt cccgtgattt ccgcagtggg tcatgatacg gacttcacca
     4981 ttgccgattt tgcggcggat ctgcgcgcac ccactcccac cgcagccgca gaactggcca
     5041 caccggacgg ccctgaatgg ctccgcaagc tgtccagaat ggaacaggct cttcatgcat
     5101 ccgctcggca ttccctcttg cgttccaagc tgaaactgga tgtttacctg cgggggaagc
     5161 tgttggatgc gtattccctg ctctctcctt attcccagcg cctggacgat atggaagaaa
     5221 ccctgcagaa tgccgcaacc acgagagttt ttcataacac gctgcatatt aacaaactgg
     5281 aacatcagct gcaaatgcgc caccccgccc acaggaaccg ggagcgcaca cagcttctgg
     5341 cttcccttca ggccggttta tcccatgcag ccgcctcccg catgacggat ttatcttcac
     5401 aactcactct tattcaagca cgtcttgaag cacacagccc ggaacagacc ctgcgccgcg
     5461 gatatgccct ggtagaaaac caggataagc agcttatccg gcagacaaac caggtgcatg
     5521 caggggaaaa attgaaaatc cgtgtttccg acggttgttt ttatgtcagg gatgacacgc
     5581 cacaataacc tgtccacaag catctagtag tgcatttttt gcactcaatc taacaaattg
     5641 tgttttttct ggattttaca ggaaatggaa cctattctaa tagaagctta tcactgcttc
     5701 tatcacccgg tcccataact tctgttcata tgccttgtaa aaacatcacc cgttacactt
     5761 acgaaacaac ggcctttcag ggctatcggt tggcgatctg taagaacaag cgtttattca
     5821 cccgttattt ctccgattct caatacgggg gcgctgacgc agcgcttcag gaagccatga
     5881 gggtgagaga taatattttc aaacttttcg aacagggcaa ccttacggtt agccaaattt
     5941 tcgcaaaata tactgtgcgc tcccgtcgtc aatccgcagc tgaccgccgt gaaaaaatgg
     6001 ttcatgggaa aagggctctc agccatgtaa gacagaaagc caaagtccgc agctgaaggt
     6061 tctgcagaag ctgtttttca gctctgcttg ag
//
LOCUS       DAWEMX010000143         6071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5131_length_6071_cov_7.817653, whole genome shotgun sequence.
ACCESSION   DAWEMX010000143 DAWEMX010000000
VERSION     DAWEMX010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5131_length_6071_cov_7.817653"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2333..2342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1522 a         1812 c         1491 g         1236 t
ORIGIN      
        1 gaatccggca aataggcctg gagcacaatc cccgcctcca gctgcatgaa ttcatcctcc
       61 atcaacgtac gtttgaacgc ttccgccgtc agatgaagat cacggtattc ctccatatcc
      121 agattcacga acttgggctt tctggaaccg tccggcaacg ttaccgcatt ggtaatggcc
      181 gcccggtaca ggatgcgcag gcgctcctga atcaatttga cggtttcttc aaaggctacc
      241 agatgaatct ggctgaaaat ggctgaaatc ttgacggaaa tatagtcaca gtccttgtct
      301 gtcagccggt ccacgacctg ctgaagacgg tggtgcgcct cgctttcacc cagaatggcc
      361 tctcccagct ggttgatatt catccggatg ccgcccttgc gcctgcggcg caggtggggg
      421 cgcagttttc catcctcggc aggaagaatt acattggagc tctcctgtct caactggctg
      481 gtaatcatgg gcatgactac acccggaaat tgagcggaaa atctcccccc cattttcatg
      541 gcgaaacggt ccgccgcaga caaataacgg ggaacgccat atccgtccaa aaggtagcgg
      601 aacaactcag caccccgcgc cggagaagaa ggacggaaca cacgatccgc cagtgccagc
      661 gtaaacgcct tgcccgccgg atcattcatc atgcgctcca tttgctgcgc ctgcctcttc
      721 tctttccctc tcatcccggc attcgactgc tttaaaatgg actccgccag ttccacagcc
      781 ttggcggcca gttgctggtc agtccattgg cctcggcgag cctccgccat catgtccggg
      841 atggatgaat ctgtcataac ggttcgaaga ctagccccgg gcaggggcaa aatcaagcca
      901 atttgcccgg gccttcctgt ggaaagggga atcacggagg aaaacgcatc ccttgattcc
      961 gtgacggaag caggggggcc ttcctgtcca tttccggcaa ctcaggaaag gacacattaa
     1021 tcataccgcg cagccgccag acgagctctt ctcagctctt ccctgagctg gtgcgcccgt
     1081 ctgccgcaaa cgctggaacg gttcatgtcc cggacctctt ccaaaaccgc aaccgcctgg
     1141 ttgaaggcat cctcgctttc cggagtgtgg gcttcccggt acagaacctc ttccagccgc
     1201 acccagaggg gatgcaccat ggagagcaga attaactggc gctcctccac ggataacgag
     1261 gggtcatccg cgcaggcacg ccacgcttcc aggcatgttc gtatggaaga agctttgcaa
     1321 gcccggaaag actcgccaag caattcggcc gttttcctgt ttttctccag acgaaaggcc
     1381 gcctcccggg cgttaagaaa agccggaacc gtacagtcca atttttccag acgcttcatg
     1441 gtgccaggat aatcccgaaa ccaggcctcc ggaggcacct gttccagttc ccggcaaatg
     1501 gcggaggctt ctgccgcagg atccccgccc ttcggaatat cattcacccg cgtcaccggc
     1561 cgcacgcgct gcgctttttc cgcaagcagg gacactcttt cccgcagaga agctgcagag
     1621 gcccctcctt ccaccaggtc gaacacacgt cctttggagt caagaaaaac cacggaaggc
     1681 agcatgcgca caccatgcat cgccgcacgg gacaggctgc cgtcttcttc ccaatccttc
     1741 acggagggat gtctaggaac cgtgacaaac tgcacgtcac gcacatattc atccagcgcg
     1801 cggcgttctt ccgtgcccgg ctggagcgct ttcaaccaac gggcttccgc tgaaccgggt
     1861 tcctctgaac cgtacacaac cagaacaggg gatggaaggg agggagccgc agaacaggag
     1921 acggaaaggg catccatttc cgcacgtccc ggaaacgcca gagccaaaaa tgcaatgacc
     1981 gctaaagtcc tcatgaataa actgccatcc atcagccctt tctgatggta atgcgcttgt
     2041 aacggctgtc cccttcttcg gactccgtca caattccttc catggactga agggcattat
     2101 gcaccaggcg gcggtggtag gcattcaacg gaggcatctt catcggcttt ccgctctcct
     2161 ggacgcgttc ggccagggaa cgggcctttt ccagcaaacg ggcttcgttg cgctcccggt
     2221 aatggtcgca atccaccttc acgcggggag catcctccat cttgcgctgg atcatgcggt
     2281 tgaccagata ctgaaggtca tccagcctgt ccccgtcacc cccaatgatg tannnnnnnn
     2341 nntgtactgt gaatccgggg aggaaatatt caggcacacg atgccgtccg tttccgttac
     2401 ttcgatatcc gcggaaaacc ccagcgaagc cagaaggctc ttcaaactct gttcagctat
     2461 ttcttgcacc gtcattactt taaattaata ggaaaagggt tccataccgg aaaaaccgcc
     2521 tcgcgccgga cggtgcgagc cttttctcca atacccgatt gacataaaaa tcataaccta
     2581 tttattcccc tcgtctatga aaatttgtgg aagttccgtt ccatatcatg tacgcaatgc
     2641 cgtccctgcc ctgccgttcc ggcaacacgg ttccgttcct ccttcggaat cagatgcaga
     2701 aaacaccgct taacaaggag gccactgttt tccgcctgcc cgcggctgta ccccggttat
     2761 ctccgcttct ccctgccgca aatgaaacac aggctgcagg catcgcagcc atgccaatca
     2821 aacaggcatt tcctcacagc ccgttcttta ttacataaaa tattaatcac cagtttttat
     2881 aaaaattttt catccgctcc ggagaaatac tgatgacaaa acaggacgga acatacccgg
     2941 aaaaagggaa gtcgaactat tacccacata gaaaaatccg gtccctcatg aacaagccaa
     3001 tgaaaaaaaa gaaaccgctc atggaagaca gcgccgcccc ttttcccaac ggaaaagaag
     3061 ctgctcccca ttatacggat acaattgatc cggaattgat caaacccact ccaaaaccta
     3121 cgccgcccaa tgcggaacct tccgcgcccg gttccatgaa gatgccggat aacgctacgg
     3181 aaaaaatcag ggagctggat gccatgcgct ccaacggcat ggaacaggcc ctcacaagca
     3241 acctgggagt taaaatatcc gatgatcaaa acacacttaa agcaggaagc agaggcccct
     3301 ctctgcttga agacttccac tttctggaga aaatggctca ttttgaccag gagcggatac
     3361 cggaacgcgt ggttcacgca agaggttccg gggcacatgg ttattttcag gtgtacaaat
     3421 ccctttccaa gtacaccaaa gcggcttttt tgcaggatcc gggagaaaaa accccggtct
     3481 ttgtgcgttt ttccaccgtg cagggcttca gaggatctcc ggatacagta agggatatcc
     3541 gcggttgggc taccaaattc tataccaagg aaggcaacta tgatctggta ggcaataaca
     3601 cgcccgtctt cttcattcag gatgccatca aatttccgga tttcgtccat gccgtcaaac
     3661 cggaacctca caatgaaatg cctcaagggc agacggccca tgactccttc tgggacttcg
     3721 tctccctgca gccggaaact ctgcacaacg tcatgtgggc catgtcggac cgcggcatcc
     3781 ccagaagctt ccgtacgatg gaagggttcg gcattcatac gtacaaactg gtcaatgaag
     3841 atggaaaaag cactttcgtc cgtttccact ggaaaccgct gtacgggaaa aaatccctgg
     3901 tgtgggatga agcccaggta ttgacaggac gcgatcctga cttccaccgc aaggatctct
     3961 ggcaatccat tgaagccgga gattatccgg aatacgagct ggggctgcaa ctcatcccgg
     4021 aagaggacgc agaccaattc gacttcgata ttctggacgc taccaagctc attccggaag
     4081 cactggttcc cgtggaaatc gtcgggaaaa tggtgctgaa ccgcaacccg gacaactttt
     4141 ttgcggaaac ggaacaggca gccttctgcc ccgccaatat cgtgccgggc attgattttt
     4201 ccgatgatcc gctgctccag ggccgcattt tctcttacag cgatacccag cggcaccggc
     4261 tgggaggagc caacttcacg gaaattccca tcaaccgccc catttgcccc ttccacaaca
     4321 accagaggga cggcttccac cgcatgcaga tagacgcctc accggccaac tatgatccca
     4381 attccatcgg gaacaactgg ccgagagaaa cgcctccgga gaaaggcggc ttcaccacca
     4441 gcccccagac ggtaagcggc gtcaaggaac gtttgcggag cccctccttc gcggaatact
     4501 attcccaccc ccggctgttc tggatgagcc aaactccggt ggaacaggag cacattatca
     4561 acgctttcag ctttgagctg ggcaaggtga cgcgccccta tatccgggaa cgcgtggtgg
     4621 accttctgac acgcattgat ccggacctgg caagcggagt ggcccgcaac ctgggaattc
     4681 aactcaccag ggagcaactc agcagggaac ttcccaggcc cgtctgcggc ctggaacaag
     4741 atccgtcatt gagcctgtat gcccatacgg acggcaacct caaaggcctt cgcgtctctt
     4801 tactggcagc ggacggcgtc agcctgaaat ccgtgaaaga aatctgcgat gccctgcatg
     4861 aagaaggcat ccacccccaa atcattgccc cgcacatggg aagcataaca acggaagaag
     4921 gggaagagct gtttgttgac ggaaccctgt ccggcactcc ttccgtcctg tttgactccg
     4981 tcatcgtccc ggaaggggaa caaagcgtca caacgctcct gaaagacgga gatgccaagt
     5041 accatttgcg ccaggcctac aggcacctga aagccatcgg actgcccggc aacgccaaag
     5101 ccatgcttga ggcagcctcc ctgccccagg atatggatga cgccgggctg ctcatgccga
     5161 aggacaccaa gtccctgatg ccccccttca tcacggccat gaaacagcac cgcgtctgga
     5221 gccgagaacc taaaaccctt gatttcggag cctagattcg tttcttatac aggaaccgct
     5281 tccatttacg ggctgccccg ccgaaaggtg cggcagccgt ttttcactaa aaaatccggg
     5341 ggagttaaac aacacttcgg gcaaattgaa aaagggaggg agatacactc aaaacgggaa
     5401 cggaaaccgg aatacagcat gatgataatg ccttcttaaa agaaaacccc gcaagccttt
     5461 aaacttgcag ggtttattaa tggtacacgg agagggattc gaacccccga ccaactgtgt
     5521 gtaagacagc cgctctacca ctgagctatc cgtgcgtgag gtggaaggaa tatataccgg
     5581 gaaagcaggc ccggcaagga gaaaatgaaa aaaatgcgtc ctcatttccc tgttctccat
     5641 gactgtaaaa tccggacacc gccaattcca gaaaatggaa agaaggcaga caaggatttc
     5701 ctgtcccttt ccgggaatat gtttccgtat tatttccgga acctgttccc ctacctgaca
     5761 tctacgcgcc ggagcgcgcg gtaatgcagg ataatgaaga gaacgcacaa tacggcttcg
     5821 aaccaataga acatgtctaa cgcatcccga ccggaagtga aaccgtagga atgcagccct
     5881 actccaagca tgttgacgcc ccaccaggca aaggcaatga taacgccgcc cactacattg
     5941 ctgaagtgga gcagccagga agacagccat ccggctttcc ggacatgcaa tacggtcaat
     6001 tgccacagta caatcatcaa tgctccgttt tccttgggat cccagcccca gaaccgcccc
     6061 caggattcat t
//
LOCUS       DAWEMX010000144         6021 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5180_length_6021_cov_12.077271, whole genome shotgun sequence.
ACCESSION   DAWEMX010000144 DAWEMX010000000
VERSION     DAWEMX010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_6021_cov_12.077271"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1472 a         1694 c         1616 g         1239 t
ORIGIN      
        1 ctggaagttt atgacctgct cgtccgtggt aaaaaactga cacaggaaga ggagcaaaaa
       61 gtcaaactgg ctgcgaaaaa tctgttcaag aaactgacag aggagaaaac cgaattgctc
      121 gttgtggact ggtacaagga tgaccaacca agaatgaagg tgaaatccgc cattgagtat
      181 gccttgaatg aggatttgcc ggatagctat gacaaggatt cctttgatgc caaaaccaac
      241 cttttgctca atcactttat agatatggca atccaaggct atggatggat tgctgcatag
      301 tacaaacaga aaaagtccac actcaaggtt ttatcctttg gcgtggtctt tacttctgtt
      361 gccgcccgaa gaaaatcggt taaaaatcag tttttgcact aatccctaac cgcaaatccc
      421 tttatccggt ttctttttta ccggattcca cgtcagctta ttcgtccacg gaacacagag
      481 cgtactgcag ccagaaagat gaaaagccga cagttggcgc agcaagtccg cacaggcgtt
      541 acgccggaat tttaagcgga ttcagctatc ttccgccgga agcggcagcc tctccggaac
      601 agaatcgttt gtttcttttt cccccaaccg cggacttccc cggttggcgg tcccgtaaaa
      661 cgcatgtccc gacgcagctc aacagaaaaa aactttcccg gctctattcc tttatcgaat
      721 ctttttgtat gaacataacc atcaaacgaa tttatgaccc caaggaaccc ggggacggat
      781 accgcgtcct ggtggaccgg ctctggcccc gcggcatttc caaggaaaaa gcttcctggg
      841 acgagtggct gaagaacata gctccatcca cggcgctccg ccagtggttt gctcatgatc
      901 cagtcagatg ggacgcattc cgcaaaaagt acgacgagga actggatgga aacggagaag
      961 ccgtttccca tctgctccgg ctggcgcgga aagggaaggt tacgctgctg tactccgcca
     1021 gaaatacgga gcacaacgaa gctgcggctc tgagggatta ccttttgcaa cggtttgctt
     1081 cataaataga agcatattcc tgcacaaact ccatccgcaa gccccaaatg ctgtcacggg
     1141 aacctatgat acggcaggat ttcttctctc gttcatgaac aagcgaagaa aatggaaagg
     1201 aggtgaatac aatatggcca gcagttccca catgggtgga agtcataagg gcagttccaa
     1261 aaaacgctcc gagactacca caagcaaaac cgggcggcgc atcaaacgcg atgcttcaac
     1321 cgggcagttt atgagcgata aaagctcagg ctccaaggaa aaacattaat gcccgcgagt
     1381 tttccgcctg attcaggctc cggatccggt ccggagcctt tttatttttc tccgcatccg
     1441 gaacacgagg aagtcttccg ccatccactc aggcccaatt cttcaaaagc ctgaaggccc
     1501 agcgcttcag gggctttccg gtctccgcga atcacaacgg caaaaggctc ttccctgccc
     1561 ggaacgtaat acgcagcccg cagaaccagt tcctctccat ccggatgggc gtaaacaccc
     1621 acgggcgtgg aacagttcgc acccagacgt accaggaaag cccgttccgc cagcgcacag
     1681 gcaaaagatt ccggatcatt gatgccttcc agcatttccg ccgtctccct gtccgaggaa
     1741 cggcattcca ttccaacgat gccctgcccc acagcaggaa taaaaacttc caccggcagg
     1801 ggacgcatgt aaagttttct tccttccaca agggtttccg gcgcgtacag ccccaagcgt
     1861 tccaaaccgg ccctggccag aatcacggca tcccagccgg gatgttccac cagctttccc
     1921 aaacgcgtag gcacattccc ccgcaggttt tcaaaccgca ggccggaacc ccagtacgtg
     1981 cgcgccatca ggcgtcggcg cacgcttccg gtagccagcg ttccggaacc gttccagtcc
     2041 ggttctttcg tgataagcac atcctccacg gcagcacggg gcaagacggc ggccagcgtg
     2101 aagccggcgg caagctcgct cggcacatct ttgaggctgt gcaccgctac gtcaatgcgg
     2161 ccatcccgga gggcggtctc cagttcctta atgaagatcc ccttgtccac catgccggaa
     2221 acgcgggcca catccgccag aggaacatcc gtgcggcggt ccccaatagt atggatgatt
     2281 tcacagcgca cgtccagttc cggataacgg agggaaaggg cagcacggac catatccgcc
     2341 tgggccaggg ccagggcgct cccgcgggtg ccgatgatga gggtattttt actggtcata
     2401 aagagcggaa tccggaaaat atctggagat gctgtcccgg atgatggcct cgcaccggga
     2461 aacctcctgc ttccggctgc gcttggcttc atccgccagc tgggtgaggg tatcaatatc
     2521 gtagagatac acttcgtcaa tatccgccac gtctggagaa atgttgcgcg gcacggaaat
     2581 atcaatcaaa aagagggagc ggtatttacg ggaggcacgc aaaggcagaa gcgtttcccg
     2641 ggtgatgatg cagtggggag cagcggtggc ggccaccacc acgtcaatat ccctgagata
     2701 gttcccccac acgtcatacc ggatggcctg gccgccgatc atgcccgcca gctctaccgc
     2761 acggtcaaag gagcggttgg ccacataaat gccctcagca ccccgggcat gaagggcgcg
     2821 ccccgtaacg cggctcatct cgcccgcccc cagaataagg acgcgggtgc cggaaagatc
     2881 cccgaaaatt tgttccgcca gctctactgc cacggatccc acggaggttg ccccggcatg
     2941 aatctggctt tccgtccgca cttttttacc gatagcaaag gctttctgaa aggttttgtt
     3001 ggcgcaggcc gcagttactc cggcatccag ggccgtctgg taagccgttt ttacctgtcc
     3061 gaagatttcc gtttccccca gcaccatgga atccaggccg ctcagcacgc ggcacagatg
     3121 gcctagagca tcttctccct gatggctgta aaaataagaa gccatatcca gttccccacg
     3181 cccatcaccc agaaaatggg ataaaatgtg ctcctgcgca ttttccggag cgttggacca
     3241 ataataaatt tccatgcggt tgcaggtgga aagcacaacg cactggtcca ctccaggaag
     3301 ggaacggatg cgctgtcctt cctcccggag tttatgggac ggcacggcaa accgctcccg
     3361 tatttccacg ggagcggtcc ggtgattcaa gcctaaacaa accattctca tcaacttacc
     3421 aggaaaatgc tcatggatgc aataaagagg gccacgcagc agcaggccaa catccttccc
     3481 ggaatgccgc ggcggtagat gaaccacagc aggacgctgt agcctgccgt gagaagaatg
     3541 acaatggagg tcttggccgc attaatgggg agattccccc gccacccaag ccattcccca
     3601 agcagaagaa ggaggaatgc cacaagaacc aggcgcttca tgctggcttc cagtgtacgg
     3661 atgggaggaa gcacacggca ggtaccggga attcggtgtc ttttaagaca ttcattctgc
     3721 atgcagaacg ccgcccccgc ggcagcggcc aggccgccgg ctccgtagga catcatggct
     3781 atgcccacat gtgccgtcac ccagaagtca tgcgtgccct gcacgtccgc tccggaacgg
     3841 cctatcagca gggaaaaaac cgtcagcacg gcaatagccg gagccgtaaa gatgccaagc
     3901 acggacattc ggtaagcacg tcccagcatc agataaaaga gattgatgct ccaggagagg
     3961 aagaagagca cctctgaagc gccgcggatg ggacacattc ccgtctggta cgctttgacg
     4021 catagcgccc ctgtctggag aacaaagagg cacgccatcc aaagatgggc atataaaaac
     4081 cgccgaccgg aagccagcag gcgccatccc agccctgtga aagccaggga gaggatgaga
     4141 gaggcaatgg cccaccagtt atcgttcata aatccggccc ggaaatggtt caggaacgca
     4201 aatcgcgctc aatgacggcg tatgcctggt ggttatggat ggattcaaaa ttttccgctt
     4261 ccacccggta ccagctgaag gcggaatgac tctgcgtttt caacgccacg ttgcgcacca
     4321 gatcttccac aaagacaggg ttttcataag ccttttccgt cacccatttt tcatccggcc
     4381 ttttcagcac gctgaacagg gagcagctcg cgcaggattc caccaggtca atcaggtctt
     4441 caatccagac gggcctggag gcaaaacgca cggaataagt aaccagcccg cgctggttgt
     4501 gggctccgta ggcactcatg gccttggagc aggggcagag cgtcgtcacg ggaacgataa
     4561 ctgtcagctt gaaatcagcc agtttgttgt tcgcttccgc ctccatctca aaccggacaa
     4621 cgtaatccat gagcccttca attccggtca caggagcgtt tttggagcgg aaaaacggga
     4681 attccatttc cacatgggaa cggcgggcgg acagacggcg gagcagttcc cgcggaatgg
     4741 ccagggccgt atgcacatcc agcaaagggc catgctggtg caacgcctcc acaaaacggc
     4801 tcatatgggt gcccttgaat tcttccggca gatccaccgc cagagaaacc gtcgccacag
     4861 tggactggat gcggttgagc ttgtcctgta tctggatggg gaagcgcaac ccttttacgc
     4921 ccacccggtc aatggaaata ttgcgcgtat ctgcctcgga ctgggtatct ttcagttcct
     4981 gcatggggaa aaaggatgat gggtggaaat aaagcgggag tctgccctgt taccggacaa
     5041 actcccgcct gcaaagacaa ttctaccgaa agagattttc cttggggctt gttacggcag
     5101 aaggttgatg gaacgggccc gcttgatttc acgggttacc ttgcggtgca tcttggcgga
     5161 cagcccggtc acacggcggg gaagaatctt gccggtttca gaggtgaact tggacagaaa
     5221 atcaggattg agcagattga cctgatccat cgggatgtcg agacggcggc gcggcatctg
     5281 gcgattgcac gtgcggaaac gaatacggcg ttctacagtc ttgggttcag tggacataat
     5341 aataaagata atcgataagc cgaaccagtt aacgcatttc acgatgaagc gtgcggcgct
     5401 tcaggaaagg attgtactta atcttttcca ggcggccggg agtacgcagg ctttttttat
     5461 tgcgcgtggt gacgtagcgg gacgtaggct ttccttcggc tttggcctcg gtgcattcga
     5521 ggatgatgat gtctcttggc atgatgaaaa tgtgttacgg gggaggattt atacactatt
     5581 cttcgtcatc gtcaagagaa acagataaat ctacatcatt ttgtcctccg gcaaagccca
     5641 gggccactct gggacggaac ctggcattct ggcctttttc cttttcccac tgggcctggc
     5701 attccacggt aagccgagca aaggggatgg cctccagacg cagaatggga atgaccttgt
     5761 atgacatttc gcagatgcca tatgttccgt tgtcaatgcg ggcgagcgcc tgttcgattt
     5821 catacaggcc gtcctgctct ttggaaagca ggctgagcgc aaaatcacgg tcataggcgt
     5881 cactgccggc atccgcctgg tgttccccgg agccggaagc ctcactgcct tccggatgat
     5941 tccggagagt atcgcgagcc acgccgctca tgttgtccag agccctgtcg cgcaagtcca
     6001 gcagatgctg gcgctgcatc t
//
LOCUS       DAWEMX010000145         5959 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5228_length_5959_cov_9.780827, whole genome shotgun sequence.
ACCESSION   DAWEMX010000145 DAWEMX010000000
VERSION     DAWEMX010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_5959_cov_9.780827"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1084 a         1611 c         1847 g         1417 t
ORIGIN      
        1 gcttggacat gtccgccttc agaatttctt ccggaggcag gccggaaagc agccggatga
       61 agacggcaat gaggcccttg gtgatgaggg aatcgctgtc tgcgtacagt ttcagtacgc
      121 cgttgtccgg ttccgtgtgt agccatacgc gggattggca tcccttgatg agggagtcct
      181 ccgttttgta ggcttcatcc agtctgggca attttttacc caggctgatg atgtattcgt
      241 agcgttccgt ccagtcctgg aacaaatcca gttcatccag caggtcttgc aggcgttctt
      301 cgtagttcat ggctcggaga ttgggtagga tgggccgggc gggttatccc cggcgggcgt
      361 ctgcaagcgt ggcgagcacg gctttctggg catgcaccct gttttccgct tcccggaaga
      421 taacgggagc aaaatgttcc agcacgtctt ccgtgatttc cttgccgcgg taggcgggca
      481 ggcagtggaa aacggagtgg ccggaggcgg catgttccag cagctccctg ttcacctggt
      541 aggggtagaa gtgtttttcc ttggacagtc cttccgattc ctgacccatg gaaagccaga
      601 catccgtgtt gatgacggaa gctcccttga cggcttccac ggggtccgtg gtgaagatga
      661 cgttctcgca ttccagatgc ctgcggaagt cttccagggg aaggtaattg gtaggggctc
      721 cgatggcgag cgtaaagccc agcctcttgg ccgcccacat ccaggagcgg gacatgttgt
      781 tgtccccgtc ccccacgaag gcgattttca tatccttcca ggaaccgggg ccgtagattt
      841 cctcaatggt aagcaggtcc gccaggatct ggcaggggtg ctcctcgtcc gtcagagcat
      901 tgatggtggg aatgccggaa aagctggcga attcctccac ctcctgctgt ccgtaagtcc
      961 ggatggcggc gccgtggatc atacgtccca gcacgcgggc cgtatccttg atgggctctc
     1021 cacgcccgag ctggatgtca tggacggaaa ggaacatggg acggcccccc agttcactga
     1081 tgcccacttc aaaggaaacg cgggtgcggg tggaggattt ggaaaagatg agcgcccagg
     1141 tctgcccctt caggggaagg cgttcgtgat ggccgcgttc cgccttaagc cggtggccca
     1201 gggcgagcag gtcccggatt tcgtctccgg taagctgttc gatggaaagg aggttgttca
     1261 tggtttttga aagggaataa aaacaggaaa gccgcaccat actccctgtt ttcttaaaag
     1321 aaaagggaat aatgcggctt tccggggagg gatggatatg cctttactta cagcctggcc
     1381 gccgcctggt ccggccacgg tacgggagcg ccttcacggg ctccggcggg attattcttc
     1441 ttgtaggtgg gcatttgggc cttgcgctcc ttgagcaggg cggtcatggt cctggccatg
     1501 gccttaacgc gttccggctg ctgttccgcc aggttgtgct cttcgctgat gtcctccttg
     1561 aggttgaaga gttcgaactt ctggtcaggg tgccagtaga taagcttcca gtccccctgg
     1621 cgcatggccg tgctggggga ataaagggag ttgtttccgt tcccttcccc ccatacgttg
     1681 ggcgtgtgga agagcagggg gcggttggga ttcatgctgc cgcctttcag cagggcaaca
     1741 aagcttttgc cgtccacaac ctgaggcgcc tgaatttttt ttgctcccgc tatttccaga
     1801 atggtgggga aaaagtcttc aatgatgacg ggagtagtgc agacgctttc cgccttcgtg
     1861 accccgggcc agtagacgat catcggttcc cggatgccgc cttcccggtt ggaacccttg
     1921 ccgaagctaa gcgggtaatt ggattccttg ttgcccatgc gcccgctgat ggcaaggccc
     1981 ccgttgtccg ccatgaagat gatgacggta tttttatcca gattgttttt tttcaggtat
     2041 tcccggatgt cgcccaggct cttgtccatc ccctggatca gagcggaata gcgtttttcg
     2101 ttgtcggacc atttgtggcc gtcattgggg ttggagtact cttccgcaaa cctcttgtca
     2161 taaccgcgca tgtcgaaggg ggcatggatg gcgtagtggg acatgtacag gtagaaaggc
     2221 ttgtccgcct ccctgggggt tttccggatg gcatccaggc gtttcagcgc ctcctgcgtc
     2281 agggcttccg tcaggaatgt gtcgttttca tagtaattat tttcatccag tccgcggaca
     2341 tggaaattcc ccgtgccgta tttccgggaa ccccggtagt ctgccgggcc gcctatttcc
     2401 gtgccggcga tattgtagtc aaagccgaag agcctgggat tggctccggg cgtatttttg
     2461 gagccgaaat gggcttttcc gcagtggatg gtggtataac cctgtttttt gagcagggcg
     2521 ggcagcccaa gcacctgggt gaagggtttt tccatcctgt actggatttt ttcttccgtc
     2581 aggggaagat gggtggttcc agatgccctg gtccctgccg gctggatgcc gttgacgctc
     2641 cagtctgcgg gagctttgag cgcttgatgg cctgcgtccg tggtttggtc acgcaggagc
     2701 gtccagttcg tgacgcggtg gcgggcggag ttcatgccgg acatgaggct gcaccggctg
     2761 ggggaacaga taggctgcgc gtaggcgttc gtgaagacca tgccctgttt acccagagcc
     2821 tccatgttgg gcgtgcggta gcgcttgttc aggaaagtag gtttgggagt gccgtcctct
     2881 tccctccaaa agggcaggga tgtatcctgc cagcccatgt cgtccaccag aaagaggatg
     2941 atgttggggc gtttcggttc tgccgcgcgg gatgctttta cggaagctgc ggaagcggag
     3001 caggcgcttc ccagcaggaa cgggatgaga aaggctgcaa tacgggaaat gttcattact
     3061 aagaaaacca gtacttatta taataacgca tgagacggac gtttctgtca aggattcgta
     3121 ttcccgcggc agtttgttcg ggaacgcccc gtttttccgg tgtggcgtgt accgggatga
     3181 aagagcctta ttcaaacagg gccggatagg atttccgggc cagggccgtg ctcaagtcca
     3241 cgatagacct gctgttgggg gcctggagcg ccttttgcgc catgtcccgg cactggccgt
     3301 agtccagatt gcggatggca taccggatga tggggacaag gtggacgccc acggacatgg
     3361 aggtggcccc caggccgatc agcaggggaa gcagggtgat gtcccccgcc atttccccac
     3421 agatggccgt gggaatgttt tcccgttctc cggcggtgat cgtcatgtcc atgatgcgta
     3481 tcacggcagg atgcgtgggg cggaacatat tggcgacgcg gttgttgacc cggtctacgg
     3541 caatagtgta ctgggtgagg tcattcgtgc ctatggagaa gaaatccacc tcgcgcgcca
     3601 gcacgtccgt catgatggcg gcgctgggca cttcaatcat gatgcccact ttcaagtccc
     3661 gcgcataggg gatgttcttc ctttccagtt cttcccggca ttcctggagg atgtgcttgg
     3721 ctgtgaccac ttccgtatag ccggagacca tggggaacat gatgccgcag ccgggcgtgt
     3781 ccgcgcaggc acggagaatg gcccggagct gctgcttgaa cagctccggg cggctgaggg
     3841 agacgcggat gccccgccag ccaaggaagg ggttcggttc cggagtgcgg agctgttcgc
     3901 acggcagctt gtcgccgccg gagtccaggg tgcggaagat gacgccgtgg ggggagcagc
     3961 cttccgccag ctttctgtag tagcgcgtct gttcttcttc gtccggcatg ccggatgggt
     4021 tgcccagcag aaagaattcc gtgcggaaca gacccacccc ttccgctccc acctccttga
     4081 tgccggaata ttcatggggg aactccacgt tgtcggacag acggatgttg cgtccgtccc
     4141 tggtgatggt gggcaggccg cgcagcgctt ccagcgcctt gtaggccttt tccttttcca
     4201 cctggaggcg gtggtagtat tcctccgttt ctttcgtggg tttcaggatg agcacgccct
     4261 tgtagccgtc caggatggcg ggggagtggc tttccactct cagaagggcc tggtcaagcc
     4321 ccacgacggc ggggatgccc atggagcggg cgagaatggc tgtgtgggaa acggaggaac
     4381 ctatttccgt ggcaaagccg tgaatcaggc tggcatccat ggcggccgtg tcggaagggg
     4441 tcaggtcgta ggccaccaga acctggtctt tttccgtttc ttcctcgtct tcaggaggtt
     4501 ccgtgctgcg gaggttgttg atgacccgct gcatgacatc ctccatgtcc gccgttttgg
     4561 cgcgcaggta ggggtcgtcc acacggcgca gaacttccat gtagttctgc atgaccacat
     4621 aaaagatgtg ctccacgttc tggaggcgct tgagcagttc cgtctgcacc tgctggagga
     4681 taattttatc ctgaaggaga aggatgtggg cgtcgaagat ggaggcatct ttctctccgg
     4741 atatctggat catgcgttcc cgcagcgtcc tgatctggtc cgcagtggtt tgcagcgccc
     4801 ggtggaagcg caccagctcg gcatccactt cttccgggga aatatcgtac gctacgggtt
     4861 ccttggaacc gctgatctga attttaatct tgcccagagc aatgccgggg gaaacgggaa
     4921 tgccctgaag ccaggtttct tccgttgaaa tgggttcctg aatcatctgc gggcttaatg
     4981 gcccggtctc atatggccgt gagccggagt gttcaatctt cttcaaactt gcgggaaatc
     5041 aggtcttcca gcgcattcag cgcttcctgt tcgtcctttc cttccgccgt gatggtgatc
     5101 ttggagccgt ggcctaccgc cagcatcatc aaacccagaa tgctcttgcc gtcaacttcc
     5161 tcgccatcct tctccacgac aatgtctgca tcaaatttgc tggccagttt gacgaattga
     5221 gcagccgggc gtgcgtgaat gccgagtttg ttgatgatgg tgagttcctt ggtgaccatg
     5281 gaatttgaag taaggggtga taacggttgc tacaacggta agtacactaa ttaaagtttt
     5341 tttccaagag ttttttgtca gtcctagttt tcggaggatt ggaagtttga catgaggcgg
     5401 cggttgaact ccccggccat gtcgatgccc atgtccttga gatgatagtc catggcggct
     5461 acttccacca gcctggcaat atccctgccg ggagccacgg aaagctgaac atggagcact
     5521 ttttccccca ggacgtccag cccctcccgc tccaggccaa gcctgtccag ctcttcctgg
     5581 tccttggcgg gaatgagggt gacgatgagg tccagacgct tgttgtggcg gatgcccagg
     5641 ccgcgcacct ccatgaagcc gcggctcatt tccggagcgc tggccaccag ttccccgccc
     5701 acgttgcgca cgtacaccat gtcgtccgcc acgagggccg cgccgcgttc aatcaggccc
     5761 agggccgttt cactcttccc cacgccgctc ttgccgcgga tgaggacgcc cacaccgcgc
     5821 acgtccacca tgcagccgtg cagggtgacg ctttccgcga attcgttttc cagaatgatg
     5881 gtggcggagt tgacgaattt catggtcacc aggctcgtgc ggaagacgca gacgccggct
     5941 tcatccgcca gctccacga
//
LOCUS       DAWEMX010000146         5928 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5259_length_5928_cov_12.262047, whole genome shotgun sequence.
ACCESSION   DAWEMX010000146 DAWEMX010000000
VERSION     DAWEMX010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5259_length_5928_cov_12.262047"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1921..1930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1211 a         1572 c         1913 g         1222 t
ORIGIN      
        1 tattcctgct acaggctggg aacgcaccgc acgggggagg ccgtcgggga aatcgagtac
       61 ggagacgatg tggcggagct ttccattctg cgggagcaca tgcgcgggca ggtggaccag
      121 atttgcctgg attacggggg cgtgaagggc ctggcgggcc ttatcgtcat cggccaggaa
      181 atgcagcagc tccgtgaccg cctggccccc acgccggaag gcaaggtggc gtgttcctcc
      241 ctggcagcgg aggcggagcg gatgtcccgc accactctgg aacagcgcat gaatgtttac
      301 ggtgcggatt ttgccggggt ggactccctg ctgcccgccg ttttgatgac ggaaatgatt
      361 gcccgcagcc tgaacctgga tgacgtcatc attcccgcga gcggttatga cgaggagttt
      421 tcaagcagcc tgatacgtgc ggaacagcat ccgggggatc tggaggcgga ggttctccat
      481 ttcgccggga ttctggcgga caggtacaag gcggacaaag ggcaccgcga gcatgtggcg
      541 cgcctgtgca tggaaatgtt tgaccagctt caggacctgc accgcctttc cgaacatgac
      601 cggctgctgc tggaagtggc ctccattctg catgaagttg ggtcttttat caaccagcag
      661 aaccaccagc tccattccca gtatatcatt ctcaacagtg aaatcttcgg cctttcccgg
      721 gatgatgtgg aaacgatcgc cctgctggcc cgctaccacc ggcatgaagt tcccgccaat
      781 tccgatccca tgtacgggga gctggaattg agggaccgca tgcgcgtagc caagatggcc
      841 gccatcctgc gcgtggcgga tgccctggaa cgcggccatg cccagcgcgt gaacggcgtc
      901 cgggcgcaca tccgcgggcg catgctggag ctggagcttc agggcgtgcg tgaaaccgcc
      961 gtggaagacc tggccctgcg gctgaagggc gacctgtttg cggatatctt cggttatgac
     1021 gtcgtgctgg cgccccagcg gtaggctgat ttaaaaaact tccattttcc atttttccca
     1081 tgtctgacga atatatcaac agagaacttt cctggctgga attcaaccag cgtgtgctgg
     1141 accaggccgt aagaacggat cttcccctcc tggaacgcct gaagtttctg gctattaccg
     1201 cctccaacct ggatgagttt ttcatggtgc gcgtgggcgg ccttcagatg ctgcggcatt
     1261 ccggttcccg cgtgaaggat atttccgggc tgacgcccac ccgccagctg aaagccatcc
     1321 gctcccgcgt gggccgcatg attgaggacc aataccgcct gttccatgag gaaattctgc
     1381 cgtggatgga aatcaacggc atcaatcccc tggcgatgga ggatctcagc acttcccaga
     1441 aattgacgct ggagcagtat ttcaacaacc agatattccc gcttctgacg ccgctggaca
     1501 tggatgctcc ggaagctccc gcgctgccat ccctcaagct gctgatgggc gtggaactgc
     1561 gcgacaatga gaccggggag gtgcgctccg ccgtggtggc ccttccggac ggcctgccgc
     1621 ggcgcgttcc cgtcccttcc gcggaggcgg agcgctacgt gatgctggag gacctggtcc
     1681 gggagtgcat cggcaccgtt tttcccggtg agaccgttct ttccgcagcg gtgttccgcg
     1741 tcacgcgcaa tggggacatt gccgtgcagg aggaagacgc cctggacctg gcggatgaga
     1801 tggaggaagt gctcgtagcc cggaaatttt ccgaatgcgt gaggctggaa gtagagtcct
     1861 ccgcgcccgc ccccctgcat gacaagatca tgcggattgt aggcgccggg aaggaggaga
     1921 nnnnnnnnnn gagcagtggt ccccgcagcc gtccccttcc gtgaatccgg ccgtcagcat
     1981 ttttgaaaac attgccggtg gggacattct gctcaatcat ccctatgaaa gttttgaacc
     2041 cgtcctgcgc ctggtggagg aggcagcgga agatccggac gtcatcgcca tcaagcaggt
     2101 gctgtaccgt accgccagga actcccgcat cgtggccgcc ctgaaaaaag cggcggagag
     2161 cggcaagcag gtgacggcgc tggtggagct gaaagcccgt tttgacgagg cccgcaatct
     2221 ggaaaaggcg gaggagcttc agcgttccgg cgtgcaggtt gtttacggcg tcaaggggtt
     2281 gaagacccat gccaagattt gcctggtggt gcgccgggaa cagggcatgc tgcggcgtta
     2341 ctgccatttc ggcacgggga actacaatga gaccactgcc aagatttaca cggatatttc
     2401 ctaccttacc tgcgacgacc agctgggagc ggacgcttcc cagttcttca attccgtgac
     2461 ggggcgcacc aagctcatgc gcttccgcaa gctgtatccc tccccggtgt tgatgaaggc
     2521 gcgccttatt gaacttattg aaggggaagc ggaacgcgcg cggcaggggg aacccgcccg
     2581 catttccgcc aagatgaaca gcctccagga cgtggaaatc attgacgccc tgtacagggc
     2641 cgcggacgcc ggggtggaca ttgaactgaa cgtgcgcggc atttgctgcc tgaaaacggg
     2701 cccccggccc aacggcaagg taatccgcgt ggtcagtatc gtggacaggt atctggagca
     2761 cgcgcgcatt ttctttttcc accacggcgg caaccaccag cttttcatcg ccagtgcgga
     2821 ctggatgacc cggaatctgg ataaaagggt ggagcttatg gtgcccgtca ccaacgggaa
     2881 gctggcgcgg cgtctgaaat ccattctgga cgcctgcttc agggacaatg tccaggcctg
     2941 caatattctg ccggacggca cttccgaaca tgtcgtccgg aaaaagggtc agaaagcctt
     3001 ccgtttgcag cagtacctga ccaaggaggc gcgccgcttg gccaaggaca aggaacggga
     3061 acgccagcag atgctggaac cgcacacgcc gcattaaagg cgctcgtcac gggcgtttga
     3121 aggtccgccg ggaacagtct ccggagtacg ccggttttcc ggccgtctgc ggaggctgcc
     3181 catgtttccc ggagaggggg gaagccggtt acggttttct gccgtcaaga atggacagca
     3241 attttttcag ctgcctgatc ttgtcccggc gtttccggaa aatatggccc cgggagaaga
     3301 acagtttcca ttgctggctg cggatatggc gctggaggag cggggccagc ccctgggaaa
     3361 tcgcatccag ttcctcccgg aacgcgggaa gcctctttgc ttccggccac cagaaaaagg
     3421 agtacaggat ccagtggaac tgccccatgg ttccgggggt gtcagggagc cgccatggtt
     3481 tttccgggcc taccatgtgc agcagtttcc atgaacgccc gcaggatatg gtcggaacgt
     3541 tttcaaattc ggttccgcgc aggagccgtg ttttttcttc atcccaggtc caggacagga
     3601 attggaagtt ccattccgga ggcagcgtcg tcatgtctcc gtgcagggcg atatttaaga
     3661 tgtcctggtc cggatagggg aagggttccg ggcggttccg aagttgttgg agcagccggt
     3721 gttccaggtt gcggcggcgc atctggtcca gatccagcac caggacgccg gaattgcagt
     3781 aggaaagggg ggaggaaagg tagttgccca tatattccag atggggatgc atcgttcctt
     3841 tttcaatgct ccagagggcc aggccgtctc cggccgccgc tacggaagct ccccgcaaat
     3901 cggaaaaata cagttcggct acgtctccgg ccagcaggac atctgcgtcc agataaacga
     3961 tcctgccgta ctgcgggaac aggctgccgg ccatcaggcg ggcatacgca aggggggagt
     4021 agcggtcctt gttgccgttt tgaaagagat ggcggaattc ttccggaagt tgcaggaaag
     4081 aaagggaaac ctgcggaaag ggggcggcaa cctggttcag ctcctgtatt attcgcgcgt
     4141 ccattccgtc gtgaacgatg tgaatgtcat aaaagcgttc ggaactggcg tgaaggcaaa
     4201 gggtatgaat ggctacgccc aggggcagtc cccagccgcc ggtggcggaa aacatgacgg
     4261 ggatgcgtga tttttccggg gatgcgggaa cgggcggggt tgtcatggca tcggggaatt
     4321 tcatgggaag ggtcttgaag aaggggctga gggaaggaat aggccgggga gggaaagagc
     4381 atggaggctc cgttcaggcc ggaaaaggga tgtctgttat ttgaagaagg gagaatctga
     4441 agtctttaaa tgccggggcc gggatttttg cggggggtaa ccgtttttcc ccgtatggaa
     4501 aataatttgg cggttttcgt tcatttgtcc gtttcatggt tcggggcggc ttttcgggca
     4561 ttcctgttcc cttggcggtt cctgccgggc ggagagaagg ggcaggagca gtccggctgt
     4621 tttccctgcc tggtacgcgc ggtccggagg ggagaaatgg aagggatcta tatatctgcg
     4681 cccctgcatg aagtcatggt ggtctgaaag aaccgcgccg ggcagggaag atgccgcctc
     4741 gcggacaatg gcattaaagg cggtgatgat tttctctttt tcttcattca gcgtgccgtc
     4801 cggcccatcc atgatggacg tactggttgc caggatgagg aaacggcagc gcttgctgag
     4861 ctgggagacg aatgccatgt aatttttgcg gaagtcatcc acggaaatgg cggagacttt
     4921 attggtgacg tgggaaccga aggaaacaat gacggcttcg tatccggcgg agggaaaata
     4981 tcctgcaaga gtggggagat agtccggatc tccgggaatc agcgtggagg agaacaagtc
     5041 aatgtcttcc tgaagttctg aggcaagggg ccgccacaaa tgccgcatga cggaaccgcc
     5101 aatgagcagt atgcgcgcct gccctttttc tgaatgaatg gccgtaatcc agtcggtttc
     5161 ctcacggtaa tggggagggg ccgccaatgt cctccagcgg gcaagcatgc gttccttccg
     5221 gcgttttctt cttttctcca gcaggcggtg tattttcccg gaacgccttc tcacccacgt
     5281 ttggccaaaa ggcagccggt ggaggacctg ttttcccaga ttccggtaag cttccgggaa
     5341 atcttcttcc ccggagccgg gcagggggcg gcattgcagc atatcgccac aaaagtcaat
     5401 gaatggaagg ttcagcttgc ctgccagcgt gcgaagaagg gcgttccagg cttcaaggac
     5461 gcccggttct tctgcgtagg ggtgccgggc gctgccgtag tcggagggcg gggatgacag
     5521 gaggatcagg cggcgcgtct gtttccgcag aaaggcgaaa agcctgcaaa gcatatcttc
     5581 ggcgctttcc atgggcccgc ccccttccgg gggcttttcc atgagcctgc cgttggccgg
     5641 ggaaagatgc aggggcggcc acatgaaaag aatgatgctg tagcgggcgc cggaaaggca
     5701 gtgctccatt tgccgccaca ggcggggatg gttgagcggg aaattgaaga tgagcgtgtc
     5761 ggcggtcagt ctggacgatt tcagatggcc atggtttttt gccagagcgg aactgccgat
     5821 caccagcatt ctgtcattgc cgggcaggag gtggtcatgt tgctggtagg aatagatgaa
     5881 cacgggaaaa acagcagtta cagggatgat cgggggaact tctttgca
//
LOCUS       DAWEMX010000147         5791 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5380_length_5791_cov_12.156206, whole genome shotgun sequence.
ACCESSION   DAWEMX010000147 DAWEMX010000000
VERSION     DAWEMX010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5380_length_5791_cov_12.156206"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          997 a         1929 c         1452 g         1413 t
ORIGIN      
        1 tgttccgccc acctctatcc ggaaggcacc accatccgtg aggagataga caacggagaa
       61 ttcccggaca ccgccctgct gggccgtacc ctgtttgaca aatacaaccg gagcctggtc
      121 atcgccggtc tggccctgct ggtggcttcc atcggcgtcg tcgtgctgag ccgccgccct
      181 tccggaaaat aaagaacccc ttaccacagc catcaccatg atacctctta cgcattatct
      241 gatcctctcc ggcgtcctgt tcgccatcgg cctgatgggg gtgattgtcc ggcgcgacat
      301 catcgtcatc ttcatgtgcc tggaaatgat gctcagtgct gccaacctgt ccctggtagc
      361 gttttcccgc gcccagggca ctatgggcct gcccaactat gacggccagg ccctctccat
      421 ctttatcctg accatcgccg cagcggaagt ggctatcggc ctggccctca tcgtctccct
      481 gtacagggcc aggcgcacgg ccagcacgca agatctcaac acgctgaaag actaagagca
      541 acacactact ccacgaacat gttcaatatt gatattcaat acacctggct tctgctcttc
      601 ctgccgctgg tcgtagccgc ctttgactgg ttcctgctga ccaaacgtcc tgacgtcgcg
      661 gccctcacct ccaccttctc ctgcctggcg accttcgtcc tttccctggg tctgctggac
      721 cggaccggat ccgattcctt ctcctggctc ccgatttccg acatcttttc cgtggacctg
      781 ggtttcgtgc tggatcccct ttcctcccgc atgatgctgg tggtcacggg catcggcctg
      841 ctggtccatg ttttctctct ggcttacatg gcggacgaca aggccaaaac acgctacttc
      901 gcatgcctga gccttttcat gttctccatg acgggcatcg tcctggcgaa caacatcgcc
      961 atgaccttta tcttctggga attggtgggc ctctcctcct atctgcttat cggccactgg
     1021 tacacacggg acactgccgc caacgccgcc aaaaaagcct tcatctgcaa ccgcgtcggg
     1081 gacttcggct tcctgatcgg cattctgact ctctgggcgc tgaccaacac gctggatttc
     1141 agcctgatgg aaatacccgc aggaatcagt gaaaacctgc tgaacattac ggtactgtgc
     1201 ctcttctgcg gagcggtggg caaatccgcc caattcccgc tgcacgtctg gcttccggac
     1261 gccatggaag gccccacccc tgtctccgcc ctgatccatg cggccaccat ggtagccgcc
     1321 ggcgtttaca tgatggtgcg cgtccaatac tccatcggca ttgaggcctt ccccgctctg
     1381 gcctgcaatg tcattgccgg catcggagcc attacggccg tcatcgccgc cttcatggcc
     1441 acccagcaga atgacatcaa acgcgtcctg gcctactcca ccctctccca gctcggctat
     1501 atggtcatgg ccctgggact tctggccggg gaggcagcca tgttccacct cttcacccac
     1561 gcatggttca aggctctgct cttcctgggc gccggtgcca tcatcttcgc ctgccaccat
     1621 gaacaggaca tctggaaaat gggcggcatc atgaagcgca tgaagctgac ttccctcacc
     1681 ttcatcattg ccaccatggc gctgattgcc attccgttca cgtccggctt cttctccaag
     1741 gaagccattc tggaagcagc ccttcgcaaa aacccggtct tcttctggat cggcgcgggc
     1801 gtagccctgc tgacaacctt ctatatgatg cgtgtcatct ttgtggtctt cttcggcaag
     1861 agccgcagcc acagttcgga acacgcctcg gaagttggcg gcctcatgct ggtgcccctg
     1921 ctgattctgg ctgtcctggc tctcatctcc ggctacgggt tcatcgcaga caggctggtt
     1981 cctttcaacg gattcgtttc cgaaagcttc cacatgggca tgcccttcta cgtctccatg
     2041 ggggcgctgg tgctcggaat cctgctggcg gctgctttct acgcaggctc cccggcttct
     2101 gacaaactct ccggcaatgc cgtttcccgg gctctcgcca accgccttta tattgatctg
     2161 ttctatgata aagtgctggt caggggagtc cagggcgttc tggccgccat catcgacttt
     2221 atggaccagt tcatcatctc cggcctcatc gttggcggac tggcgcggtt gaccgccgcc
     2281 ataggctccc tgctgcgccg cctgcagtcc ggcaacatgg ccgcctatac ggccctcttc
     2341 ggcattggcc tgctcctggt catctacttt atcgtcttct attcctgcta acctaaccaa
     2401 ccaatcacat accatgctta tctggcttgt tctcattcct ctgattgccg cggccctcat
     2461 cggtctgtgc aaggcgcccg cacgcccgac agccctgctc agcgcctccc tgacgctggc
     2521 cctcggcatc tgggccctgg ttagctttga cggctgttct tcctgctggt cccgctttga
     2581 agggatggac ctccagctca cgttggcccc ggccctgtcc aaagtaatgc tgttgctgac
     2641 catcctggtt accttcgcca ccgtgttggg caccaacccg ccccaggggg gggaagcttc
     2701 ctggtacaac tccgcccttc tgatttccgc aggcgccacc ggggcgttcc tgtccgacaa
     2761 cataatctcc ttcttcgcct tccatgaact ggctcttatc cccaccttcg tgatgatcgg
     2821 cctgtatgga cgcggcgacc gccgcaccac ggcctggcgc gccacgcttt acctggggct
     2881 tgcctccatg gtgctgctgg cagccctgct gatgatcggc acgcaggctg gcttcacctt
     2941 ctccggcctg aaagacttca tggccagcgg gcgcgaactt gcccacgcgg aactcatcgg
     3001 ggccctgctc atcgccgggt tcggcacgct gatttccctc ttccccttcc attcctgggc
     3061 agcccccgca tacgcatccg ctcccgctcc agtagcgatg atgcatgccg gagtacttaa
     3121 gaaatttggc ctgtacgggc tcttcatgtt ccagcccctg atggaaacag gattccttcc
     3181 ctggacgaat atcctgctgg tcctgctcgt ctgcaatgtc atttgggtgg ggtacgtgac
     3241 cgtcaaccag aaaaggctgg acctgctgct gggcaactct tccgtgatgc acatgggcta
     3301 tatcttcctg gctttcgccg ccctggtagt ttccggttcc gcggaagcca atccctgggc
     3361 gctgaaaggg gctgctctgc tgatgctggc ccatggcctg acaatcgccc ttctctttct
     3421 gctctgcggg cagattgaaa agcagacagg cacactggaa atcaactctc tgggcggact
     3481 gagcaccagg ctgccgcgca tggctttcgt tttcggcctg gcgggcatgg cttccatcgg
     3541 cctccccggc ctcgccaatt tcccggggga attcatggtc ttcttctccg gtttcgccgg
     3601 tttcagcaac ggcttcggcc ccgttcaaat cgccaccatc ctgtgcctct ggggactggt
     3661 catcggcgcg gtatacatgc tgagggccta ccgcaatatt ttccagggag atttgtccaa
     3721 agcctccgcc tacgccacgg aactgcagcc ctccgaacgg gcggccaact gcttcctggt
     3781 catcgccctg gcggtcttcg gcttcttccc cacgctggtg ctccagtttt tctcctgatt
     3841 tcctcccttc cgaatacaat cctagtctaa cctgattctc ttttacccat gcaagcgtat
     3901 attccggaat tcatcctggc cggcctggca atgcttttgc ttctggcgga gaccttctgc
     3961 aaaaaaacgc ccaaatttat tttcgggctg attggcgcgg cgggaaccct ggcgatgctt
     4021 cccttctaca tggaagggct ctatgacaac atctacatca tcctggcgct cgtcgccaca
     4081 gcagttaccc tgttgctttc cgtagatttc cgggccgtca tcaacctttc ctccaatgat
     4141 tccaaatccc aggacggaac gggggaattc tacatcctgc ccctgctggc ctgcgtaggc
     4201 atcacctccc tgtgcaaggc atccaacctg gtggaactgt tcgtctccct ggaagtgctc
     4261 accctgagtt ccttcatcat ggtaggctac ttccggcgga acctgggttc cacggaagcg
     4321 ggcatcaagt acctgattct aggcgctgtc agcacggggt ttctcgtttt cggcctggcc
     4381 tggtatttcg gcattacggg aacattcatt tataatgaaa cgatcgtcag ccacgccctg
     4441 gccggccaga cagcccccgc catgtacctg gccctcgcca tgctgctgct cggtacagca
     4501 ttcaaaatcg gtgcagttcc catgcagttg tggattccgg atgtgtacca gggcgctccc
     4561 actccggtga cagctttcct ttccgtagcc tccaaggtag ccggattcgc cctgttcggc
     4621 atcatcctgg ccccattcgc cgtgctgcct cccgtcgaat tcgtcgtagc cctgatggcc
     4681 gccgccaccc tgctggtggg caacctgggc gccattcccc agacgaacct gaaacgcatg
     4741 atggggtatt cttccattgc acaggcaggc ttcattcttc ccctgtttat tggtactgtg
     4801 gacggccagc tggctccgaa cgccccgttc tacctggctg tgtacctggt catgaccttc
     4861 ggcgcctttt tcgcccttgc catgatccgc atccagcgcg ggagcgagga aatttccgcg
     4921 ttccgcggtc tgggcaaaac caatccccgg ctggcgctgg cggtcaccat catgttcgcc
     4981 tccctggcgg gcgtgccgct gacggccgga tttttcgcca aaatgatttc tttcgtgcac
     5041 gtcatcaata cgggtctgta tctgggttgg atgctccccg tcatgatcgt ctgcgctgca
     5101 tccgggtttt actattactt caaggtcatc cgctccatgt actgggacaa gcccgcagaa
     5161 aacgcagaac ccgtgcaggt accggccatt tccggagtca tgctggccgc gttttccatc
     5221 ttcatcgtgc tggggggcct gatgcccctg ttcctgaacc ccatccggta acaaacgcgt
     5281 ccttttcccc cattcttccg aaggccgttt cccgaatttt tcgcagaacg gccttcccca
     5341 ttcaggcaaa gctgtatgtt ccgcgtcggc tggctgaaag cgggaagggg aaggagagag
     5401 tgcggctgcc cggccttccg gacttacggc ttcagccgtt tcagaatctt gagcaaccag
     5461 cctgagccaa ttatttcttt tatcacattc atgccggaaa aattttgaaa aaagtgcggc
     5521 ctgcctgcga gatttccccc aggccaaacg taattagcct gtcctaatta tttgcctgtc
     5581 ccccaccctc cgggcatcaa atcagacaac catcaatcat tcaaaaccat gcgtctttta
     5641 tcctccgccg ccgtcatttg cgccgccctg ttctccggaa ccatccttca tgcgcaggac
     5701 ccggaactgc tggccaagcg ccagtgccgt tccgtccata tcaaccagca ggggcatccc
     5761 tcccaggcaa gcgccctgta caatgtagtg a
//
LOCUS       DAWEMX010000148         5788 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5383_length_5788_cov_10.384267, whole genome shotgun sequence.
ACCESSION   DAWEMX010000148 DAWEMX010000000
VERSION     DAWEMX010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5383_length_5788_cov_10.384267"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2991..3051
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5558..5567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1349 a         1696 c         1559 g         1113 t
ORIGIN      
        1 tgccagtctc cggaagccag gccgtaagcc aacacagtgc caacgacgac agtaattacg
       61 ggaatgcagg tggaaatcat gccgatgccg atgccggaaa taatgacggt ggccggcccc
      121 gtcttcgcgc tgtgggcgat tttacggcag ggataggaat catgggaagt gtaatgctcc
      181 gtgctcttgc cgatgacgat gcccaccgcc aggccggtga cgatggcccc ccagatgccg
      241 gcccagttat ccaggccgat gagctggagc aggaatgcgg aagcaatggc aatcagaaag
      301 gaactcaagt tgatgccgcg gttcagggct gccatcagct ccgtttggga agcgccgcgc
      361 ttcacgcgca ccgcgtacac gcccaacagg gaaagaatgg tgccgagggc ggcgatcagc
      421 atgggtgtaa ggacggcctt gatcgccatt tcaggaacgg ccatgtaagc tgccgcaccc
      481 aaagcggcgg aagcaagaat ggaaccgcag taagattcgt aaaggtccgc ccccatgccg
      541 gccacgtccc ccacgttatc gcccacgtta tccgcaatgg tggctgggtt gcgggggtcg
      601 tcctccggaa tgccggcctc caccttcccc accaggtcgg cccctacatc cgccgccttg
      661 gtgaaaatgc cgccgcccac acgggcaaac agcgcctgga gggaagcccc catgccgaac
      721 gtcagcatgg tggtagtaat gacgaccagg cgcatggaaa gctccatgtc cccgtaaaac
      781 caatccagaa cccagtacca ggcaccgata tcaagcatgg caaggcccac gacggtcagg
      841 cctagcacgg cgcccgagcg gaaagctatt tgcagcccgg catccaggga atggcggcag
      901 gcattcgcca cgcggccgga agcatacgta gccgtcttca ttccgatgta gccggccaga
      961 ccggagaaaa agccgcctga aatgaaagcg aagggaaccc aggggttctg aatgtggagg
     1021 cagtaggcca gaaaagcgaa gacaacagta atgagcacaa agaaaatggc gacaatctta
     1081 tactgctgcc gcaggtaggc catggcgccg ctacggacat gccgggcgat cattttcatg
     1141 acctcgttcc cctcatcctg cgctttcatg cggtaaaaaa agatgcccgc aaaaatgagg
     1201 gccagcacgg aagccgatgg gatgagccaa aataaatcct ggatggttat cattgtcagt
     1261 acactttaaa ttaaagtagt aaaaacataa caccagctct actttactcc aagaaaaaac
     1321 ctgtgtccgg aacgggaaat gccccttcca gcagaaagac cagcaccacg ggaaaaacag
     1381 tgcctatcta tccgcatctg cgtcaatcag agagagctcc tccggcgtaa acggcgcact
     1441 atccagtgct ttcaggcaat ccctgatttg cgaggggcgg ctggctccaa tcagggccgt
     1501 ggtcactacg ggatctttca gcacccaggc cagagccatc tgggcaaggc tctggccccg
     1561 gctccgggcc agggcggcca ggcggcgaat tttatcccca tgctgaagaa cccgctcctt
     1621 tgttaaaaag acggaggaac ccgccgcgcg ggaatcttcg ggaatgcctt ccagataacg
     1681 gtccgtcaac atcccctggg ccaggggaga gaaagcggcg cacccggtac ccgtttcccg
     1741 aacagcctga aaaacggatt tctccggttc ccgattaaac atattgtacc ggatctgatg
     1801 caccaggcac ggaacgccgg cctccccaag catggagcac atctgcctgg cctgttccgc
     1861 cgggaacttg gaaataccgg catacagggc ctttccggag cgaaccgccg taacaagggc
     1921 tcccgccgtt tcttcaaggg gagtttccgg atcataccgg tgcgcgtaga aaatatccac
     1981 ataatccagc ttcatgcggg aaagggactg gtccaggctg gcaagcatgt gtttgcggga
     2041 tccccaatcc ccatacgggc cgggccacat cagatgcccc gccttggttg caataacaag
     2101 ttcatcacgg aaacgggaca aatcctgcat gaaaatacgg ccaaagcatt cttccgccga
     2161 accgggagga ggaccgtaat tattcgccag gtcaaaatac gtgataccgg catcaaacgc
     2221 gccgtgaata agccgacggg cgttctcaaa atcatccgca gcgccaaaat tatgccaaag
     2281 ccccagggaa acgggaggaa gcagcagccc gctgcgcccg cagcggcggt aggctgcgtg
     2341 gctatagcgg ccgggatctg gaagataatt catcggaaag aaaaaagggg ggggaattac
     2401 cgtggctgag acggacgccc atgccgtacg ccggaacgcc atgcttccgt acggtatttg
     2461 gaagccagca gttcctccac gcgggcattc atgccgggga acaattccgc gtccacgcac
     2521 agggaatgct tttccgccag gcgggcggcc agcaattcat cccatccgga agggagaaga
     2581 caattctgga tgctcccctg gtgaagaacg cctccgcgga ctcttctctg gctgccgcca
     2641 gccagttttt ccccgccggg aaggacaaga tcactcgtta cggggctgga aaaacaggcc
     2701 cgccctccgt ccggggcatc cccgtccagc atcacgcact ccaccccgca atccctcagg
     2761 acgcgggcca gcgccccatg aatccaacgg tacaaaaccg cgctggaagg acgctctttt
     2821 tctcccttgc ggtgcatggc cagagtaaag ggaatatcct gccgatggtc tacaatcccc
     2881 ccccctgtcc agcggcggac aaaatgaagc tcctcacccg gaaaaatggc gcgcgccgtg
     2941 gcctcctcat caaaataacc gtaagtcacg gtcggttccg cccagcggta nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcttcgtcc
     3061 acggccatgg cttcctcccc gcttctggcg acaggatcgc gccacagcac taattccggc
     3121 agaggggata aattgccgac gatatttgag aaacccaaca tatggcaact acagaacaat
     3181 taatggcagg aacgctccag gcaaaaagca acagtggccg attccgccct ccccggtttc
     3241 ccggatacag gcctaacggt tcatcggcaa tgcaatcctc tgggtgaaag accgggtata
     3301 acgttcgaaa aattcctcag gagagggagc gggacgcttt ttctgccgga tctggtccac
     3361 aagagccatg ccttcatccg tcaccaccgt catggaaatt gtcacggcag taacgcggcc
     3421 ggatttcaaa tcatcaatat tcacgctgct gcctatgatg tccaggcgat tgccatacac
     3481 atccatctgg gtgcaggagc cgagacggtt ggcgcccgta gtcatgaccg gaaccagcac
     3541 ggatgctctt tgcgaggcat ccttgtccga tttgttgctg ccggaattgc cgatcttctt
     3601 ctggtaatcc acttcaaaaa tcagcgtcat ttccacaata ttttccacca ggaagttgga
     3661 agggacggtt tcatcctgct ggtatcgtgc ataagcggac catagatcct gctggcccag
     3721 aaggttccgc acggtatcct ccgcgctgat taaattgcgg tacagggaat aaacgggaaa
     3781 accgtcagac tccgccgtag cgtcctgatc cagaatctgg tcacggtaaa gaagcttgta
     3841 ccccacgcaa ttcacatcgc cgatgatgcg gtcattccgc atgtccatgc tggacttggc
     3901 cggattgcgg tccgtagccg tggtaaagaa aaccaattgg gaggcattcg tgattttggc
     3961 gccttccggc cccatggtca tcaggttcac gcgggcttca ttcagggagg aggagcggcc
     4021 ctttttcctg gaaaccttgg tctttcccgc acgggaaacc aaatcgctgt cccgctgcac
     4081 cagcatccac tcaaagggat tcccgggacg gaactgcatg ctttcaaaat ccttggtcat
     4141 ggtatccatg gccgtgcggg caagcgtcgt ggtccgcaca tcctgcagca cccatctcca
     4201 gatatccacc ccgcgggaag tcagggccac aattaccaga accagcacgg tagtgatggt
     4261 catggctgcc aggagctcaa tcagggtgaa cgcgccggat ttgattttgg caaatgtctt
     4321 catgtttttt caaaggtctt cagataatgc ccgctaacgg cggaaagcga gattgcgcgt
     4381 aaacacggga gtctttaaat tttcccacgt cagatgttgt aaacgctcgg ggtccgtatt
     4441 caacagctgg aaaaactccg cctggtacag gacttccgcc ccccacggca aattcaggcc
     4501 gccgcctttc tccggcgtat aaacatacag ggaaggttcc ttaatcacct tgccgcggtt
     4561 gtaaggatta taaatcgtcc cgcacttggg agccagcttg tacccgtagg agcttgaatc
     4621 catgcgctcc actaccagct gggtcatgcg cacggcgaag acgggaccga caagggcctt
     4681 gaaatcatcc caacgctctt tgttgttcgc caggcagacc cctgtcacca ccgcagtatt
     4741 tttgccggga atgcttccgc catcctccag ccagggctgg ggagttccgt ctttgcgggc
     4801 ccccttggtc aaatccgccc tgtaattgta aacaagaatg gtcgtagcgg gcctggacgt
     4861 gaactgcatc cagtaaaacg ccttgtcaaa agcggaaaca tatctggagg aggaagactt
     4921 tctggcattg gacgcggcac gctcggaatt gcggagttct ccaagttccg ccgtcagggc
     4981 gtggacgatg cggtccgctt cacgaatagt cagggcggac tgaaccgtcc ttctggcagg
     5041 aataaacatg gccatgaaca ctacaatcag cacggccacc acgccgatcg caagcaccac
     5101 ttccgtcagg gtgaacccct tggcacgaga ctgtttcata cacttggtca acataaaatg
     5161 ggaaatccgg tattatatca cagaaactaa ttgggaatca cctgctccgt cgtcctcaga
     5221 cggctgatat tgcctttggg ataaatcaca atgccgcctg ccaggcccgg tttgccgtcc
     5281 accatcgggg ttggcctctc ctccttgccg tttccggcgg aaccggcaac caccaccagg
     5341 cgggtggggg cgttcggttg ggacatgcgg ccttcctcgt caaattcaat gaagtaaata
     5401 tccgtcattc tgttgccggg aaggttgacg gtatcctgcc cgtcacgact ggcaaaatcg
     5461 cgggccgtgg atttctcgcc tctggctttc ttcgagcctt ccagaagggt gctgtaggtc
     5521 ggactgacgt aaaaaccggc gggaagagtc tgtaaacnnn nnnnnnntaa acggttcacc
     5581 agatgccatt tcccggtgcg ggtatttttg gacatcacgc ccaaagtgcg catattgcgg
     5641 gcttcgtcat cgggagtgct cacaataatc aggcggctcc acgttccttt gcccatggca
     5701 tccagccgcg cctcctgcac catgccgtcc accatttcaa cggcggcctg aagcccctgc
     5761 cccctgcccc cgcccctcaa catgctgg
//
LOCUS       DAWEMX010000149         5741 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5434_length_5741_cov_13.483468, whole genome shotgun sequence.
ACCESSION   DAWEMX010000149 DAWEMX010000000
VERSION     DAWEMX010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5434_length_5741_cov_13.483468"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1192 a         1509 c         1781 g         1259 t
ORIGIN      
        1 cgcgcaacat aagttccttg gtataaaaat caacccttga ccccggattt ggaatccggt
       61 atgaacgtga tgccttatta ataaacagaa aaggacatgg tcctcttcat gtttgacgct
      121 ttcttttccg tgctttttct tcatgaaccc ttcggaactc ccttccatca gcattatcct
      181 gaccgggcat aatgaggaga gcagcatcag ggatgctatc cggagcgtat tcgggcagga
      241 ttatgaaggg ccggtggaaa tcatcctttc ggacgacggc tcttccgacg gaaccttctc
      301 cgtcatgcag gaaatggcgg ctcaatacag ggggccgtac cgggtaatct taaaccggaa
      361 tgaaacgccg ctggggcgcg ggcctcatat ccggcaggcg gttggtctgg cttcccatga
      421 atggattctc cgtcaggatg gggacgattg ttccttcccc tggagatgca ggctttttgc
      481 ctgggcagtg atggaacgcc cggatgccgt ggctgtggtc agccagatga ccagcgtcta
      541 tgaggaaccg ggcattgcgt ttgaattccc ggcctttcct gctgctccgc gggaaaggcc
      601 ggctgttgtg cttcaggcgg gagctttttc ttcttccgcc cattacggag gaagcatgat
      661 gatcaggaag agcgcctgcg aatggggtag ctccctgcgg atgacggcca gttttgaaga
      721 cgacttgatg gggttccacg catggcttca gggcaacatt tatgaaatgc cgggcatatc
      781 cttatattat taccggtttg cgcttaaaaa tatttgcgct gtcaacagcg cgttccggtt
      841 tgccgacatg cgggcggcgg cggcctttga aaaaagggag ctggaaatga aaagccagtt
      901 gaaaaaaagc aaggaggcgg ggttggaact gtgcggggaa ttgctggagg gccttgctcc
      961 ggtttttcgc tcgcgggaag aactgcttgc cgaaatggag gagaggagaa aaatcataga
     1021 cagcattgac cggcagcaga attggtggag ttatcccttt cggcggagat gggcgttgag
     1081 gcggccggga tggatgggta tcgtccactg cctgccgcaa aagctgtatt tattctgcat
     1141 ggttttcttt ttcaagctca ggaaagtgaa acgggccgtt tgcggtgccc ggcaggattt
     1201 ggaataaggg taaggaaacg cttgaagaag gaggggaact tttgctcgga cgggttctcc
     1261 ccggttcggt gttgtttttg atggagaggg gggcggaaga tggcttctcc ggctcgaaag
     1321 aagccggcgt gcggactggg aaggtggcct gttccggctg ggattgacgg gaaaacagct
     1381 ggaaccgtca tgagggaagc cgggaggggg agccggagtg atgatgcgtg gcgggttttc
     1441 tccggaaaaa gaaaaagcgc ctttccagaa aaaatggaaa ggcgcggtga caggttcaag
     1501 ggggagatcc tgaattagta cacgtcccgc tggtaacggc ggtgctgttt catgtccgcc
     1561 acatatgccg cggcttcctc cggagtttta tggccccagg tctggatgac ttcatgaagg
     1621 gcgttgtcca catccttcgc catgcgggag gcgtccccgc agacgtagaa gcaggcgccg
     1681 gcttccagcc atttccagag ctcttcgcct tcctggacca tgaggtgctg cacatacact
     1741 ttcttttcct ggtctcggga ccaggccacg gacaacttca gtttgccggt gccggtcaac
     1801 tggttcagct cgtcttcata acagaagtcg gtggccttgt acggattgcc gaagaacagc
     1861 cagttgccgc ccttggcgcc ggaagcggcg cgttcctccc agaatgcccg gaaaggggcg
     1921 atgccggtgc cggggcccac catgatgatg ggggtgtccc cgtcttccgg aaggcggaaa
     1981 ttcttgttgg tatgtacgaa taccctggcc gtatggcccg gctggaggcg gtccgccata
     2041 taggtggagc aaacgccgcc gcgcttgcgg tcacgggccg tatagcgtac cgcgcccacg
     2101 cacaggtgaa cctgttcggg atgggcgttc gggctggagg cgatggaata aaggcgcggc
     2161 atgatctttt tcaggatgcc gacaaaagcc tccgcggatt cgaaggaagc ggggtattcc
     2221 gtggccagat ccagcacgtc ccggccccac aggaaatcgc tgagggcgtc cttatccccg
     2281 gccagaagcg tgtccagctc ctggctgccg gaggcggcgg cccacttggt cagcagggct
     2341 ttgttgacat tggtaatgtc atagcagtga accagggcgt ctttcaggct tgcggtttcc
     2401 ccttcgggcg tgggaacctg ggaatccggg ctgaggccga gcctgtcaat cagggcgtcc
     2461 gccagggagg ggtcattggc gggaatgacg gccagggcgt ccccggcaat ataattcatc
     2521 ccggaaccgt ccaggctgta ttcaatgtgg atggtttcct tggggctgcc ttccccggtg
     2581 atatgcctga tggagaggac gggagcggga aacggatttt ttttgccgta tggttcagtg
     2641 gtcatgatga tggagggggg gtgaaaaaga atattaccag cggataacgg cggcggacca
     2701 ggtaagcccg gcgccgaatg tgaccaggac gacgttgtct cccttgccga atctgccgga
     2761 acggcgcgct tcgtcaaagg cgatggcgac ggcggctgcg gacgtgttgc cgtacttctc
     2821 aatgttcacg aacaccttat cttcaggaat ggaaaggcgc tgcgctacgg cgttaatgat
     2881 gcggagattg gcctggtggg gaataatcag ctggatatcg tccgccgtga ggccggcgcg
     2941 ttcaatgacg ctggcggcgg aagccttcat gcgcgtgacg gcatggcgga aggtctcatt
     3001 gccgcgcatg gccagggtag ccaggcgttc atgggcgttt tccgctgtgg tggggcagcg
     3061 ggagccgccg cccggaatct ggagcaggtc atgcagggag ccgtccgaac cgatatccgt
     3121 ggcaaggaca gaaccttccc cttcttcccc tgtggaagcc tggagcacgg ctgccccggc
     3181 gccgtccccg aacaggacgc aggtgctgcg gtcctcccag ttgacgacgg tggaaagttt
     3241 ttccgcggcg atgatgagcg ccgttttcat ctggccggtg ccgatgtact ggacggcggt
     3301 cttcatggcg aacaggaagc cggagcaggc ggcggaaatg tcaaaggcgg cggcattctt
     3361 ggcgccaagg gcatcctgga tatagcagga agtggaaggg gtgaacgtat ccggcgtgat
     3421 ggtggcgacg ataatcaggt caatatcctc cggcttcagc ccgcaggatt ccagagcgcg
     3481 ctgggcggcc ttcgtcccca tatgggaagt gtactcgtca ggggcggcga tgcggcgttc
     3541 cacaatgccc gtgcgttcaa taatccattc gtgggaagtg tccaccatct tttccagatc
     3601 cgcattggtc aggcggcgtt ccggaacata ggaacccgta ccgataatct tgacgggcag
     3661 tcttttaaag ggaatatcag aatgtgcggc catagctttg ggcgtatgat gaacagaaaa
     3721 cgtcctgacg ttttatgtgg aaacagtcta aaagaaagag ggcctgatga ccagcataaa
     3781 acgcaggact cctttcatgt cgtaaatgaa tggcttacaa cagccacccc tggccgttgg
     3841 cgggcggcgc ggctcccatc ttgagatagg cggagggcat cgccacgcgc cctctggggg
     3901 tgcggctgat atacccctgc ataatcagga aaggctcgtg cacgtcctcc agcgtggaag
     3961 cgtcctcccc tacggccacc gccagggagg aaaggcccac cgggccgccg ttgaacttgt
     4021 aaatcatcgc ttccagcagg cgcttgtcca tctcgtccag gccgtcgtcg tcaatctcaa
     4081 tcatggtcag ggcgtcgtgc gccaactgct ccgtaatcac gccgtcgccg cgcacctggg
     4141 cgtaatcccg cacccagcgc agcagggaat tggccacgcg gggcgtgccg cgggagcgca
     4201 gcgcgatctg cagggcgccc tccgggtcca cgggcacgtt cagcagcccc gcggaccgtt
     4261 cgataatggc gcacagttcc tcccgcgtat aataatccag ccggttcacc aggccgaagc
     4321 gggaacgcag cgggctggtc agcatcccgg cgcgggtggt ggcccccacc agggtgaact
     4381 tgggcaggtt gagctggatg gagcgggcgt tcggcccctg gtcgatgata atatccagcc
     4441 ggaaatcctc catggccggg tacagatact cctcaatggc cgggtgcagg cggtggatct
     4501 cgtcaataaa cagaatatcc cccttttcca gattggtcag cacgccggcc aggtctcccg
     4561 ccttttcaat ctgggggccg gaagtcgtat gaatgcggca gccgacggca tttgcgataa
     4621 tattggcaag cgtcgtcttc cccaggcccg gcgggccgct caacaggata tggtccagca
     4681 cgtcgtcgcg ctgtctggcg gcttccacca tcagcatcag gcggtcctta atcttctcct
     4741 ggccgcagaa ctcgctgaaa gcgggcggtc tgagggacaa atcgaaggcg gaggaggggg
     4801 cctccgtggt gcgtgtgtaa aaggactctc ccatgcgcag gactagcaaa cccgtctttg
     4861 gggcatccgt caagggggaa gtgcggtcgg gaaacgcctt ttccgggaca tgccggaagc
     4921 ctttggcttt ttttgaggac gggaggaatg cggagccgcg gaaatgatga tacgcggaga
     4981 aacggcatgt cccgccttct cttcttacag catggattcc catttttgcc gcgccttccc
     5041 ggaaactctc ggaaccctgg gcttccggaa tccttcagtc ttctcttccc gtctcgctcc
     5101 tgaaacttct caaacggctg ctggttaatc ctggagcccg gacggaaaga agcttatcaa
     5161 tagcggttct tacctgaacc ttccccgatt gcacggaagc cgtaaaggtg gagaaagaga
     5221 aaaattctta tttgctccgc aggaaaaaca gggcagggag ggtgaaaatc tgccatgccc
     5281 acatggataa tttttgtgaa ccacggaaac ctgttccttt caaaacttct gaaaatacgc
     5341 ttcgtttttg tttatgcccg ttgaagattt tctcgcgctg tcggggcgct tttcatcctc
     5401 ttcgagcaac agccggaaac gtgaaaaaac tcgggggaaa acggaaaatt ccttccggat
     5461 gaaaaaaaga ttgccaaacc tctcttctgc cgttattcta tccctgcttt cctatctatc
     5521 acaaacaatc tccaacgggc aggtgtcaga gtggtcgaat gagcacgcct ggaaagtgtg
     5581 tgtaccgcaa accggtaccg agggttcgaa tccctccctg tccgccattt tattcttttt
     5641 aaaaccttgc aacgctcttg attttgcaag gctttttcat atcgtgattg cgcaatattt
     5701 gtccgttttt gcaaaaaagt acatattagg tcgtattgat t
//
LOCUS       DAWEMX010000150         5716 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5466_length_5716_cov_10.339163, whole genome shotgun sequence.
ACCESSION   DAWEMX010000150 DAWEMX010000000
VERSION     DAWEMX010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5466_length_5716_cov_10.339163"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1212 a         1649 c         1761 g         1094 t
ORIGIN      
        1 gcaggcggcg gccagggcct caatgcaggt gcgggtgacg ttgttgaacg ggtcctgttc
       61 cgtgagggac cagccggaag tctgggagtg cgtgcgcaaa gccagggact tcgggttctt
      121 gggattgaac tgcttgatga gtttggccca cagcacgcgg gctgcgcgca ttttggccac
      181 ttccatgaag taattcttgc cctgggccca gaagaaggaa agacgcggcg cgaaggcgtc
      241 aatgtcaatg cccgccttga tcccggtgcg gacatattcc aggccgtccg caagggtgta
      301 ggccatttcc aggtccgccg tagcgccggc ctcctgcatg tggtagccgg agatggaaat
      361 ggagttgaac ttgggcatgt acttggaggt gaactcaaag atgtccgcaa taatcctcat
      421 ggagggatca ggcgggtaaa tgtaggtgtt gcgcaccatg tattccttga gaatgtcatt
      481 ctggatcgtg ccggagagct ggtccagcgt gcagccctgt tcctgggcag cgacgatgta
      541 gaaggccagc acgggcagca cggcgccgtt catggtcatg gaaacggaca ttttgtccag
      601 gggaatgccc ttgaagagga tttccatgtc caggatggag tccacggcca cgccggcctt
      661 gcccacgtca cccaccacgc gggggtggtc ggagtcatag ccgcggtgcg tcgccaggtc
      721 aaaggcaatg gaaaggccct tctggcccgc cgccaggttg cggcggtaga aggcattgga
      781 ttcctcagcc gtggagaagc cggcgtactg gcgcaccgtc caggggcgcg ccacatacat
      841 ggtggcatac ggtccgcgga ggttgggagc cacaccggcc gtaaagtcca ggtgcaggca
      901 gtcgtcgtac acgctttccg agtaggtggg cggcacggga atttgttcat tggtcatcca
      961 ggtttccgtt tcggaagccg gggacggggc accagccttg atttcaggat aggaggcgga
     1021 tgcaaaatca ggaatattgc tcattgggtg gaataggttg gtggttatta aaggccgatt
     1081 ttgttttgaa tggcttcaac ggtgttgtag cagttggcct tgacgtggat gaagatgtcc
     1141 acgccagctt ccttgaatgt ttccacatag tcggccggat aaccggcaag ggccaccatc
     1201 atgtccggtc tggtctccct gagagccttg cagaaggcgg ggacgatttc cggataagtg
     1261 tcatccgtgg agcagatgac gcacacggcg cagccgcttt ccgcagccgc cctggccgcg
     1321 tcttccggag tttggaagcc ggccggataa accacatcca gaccgccggc gcgcaggaag
     1381 tcacgggaga agtccgcgcg ggccttatgt tggcgaagaa gaccatcggg cgggaaccct
     1441 tttctgccac ccaggcgtca gccttggcga gcagggattc aaaacgttcc gccaggcggc
     1501 gcttcggcag ggcttccatt tccaggggtt cgccgcactt gcagtcagcg ccggccacca
     1561 gggccttgtt gatgaggcag gtggaaaagc cttcttcggc ggccttgcag gcggagtcca
     1621 cgctcatttc cacgaccggg agctgaatat ccgcattttt gcagcagccg tggcccttgt
     1681 gggcggaaca gcaggagcct tccggagctt ccagcttttt ctccgccagg ttgacatact
     1741 ggttgacgcc cacgatggac tgccggcgct ggtccgccag ctcgtaacgc ctggcggcgg
     1801 tggcgttcac cttcttctgg atgctgccgg ctttgagggc cttgacgatg ccgccttcct
     1861 tttcgatgcc ctggaagaat tcccatatct tccgggaggc ttcatccgtc aggttttcca
     1921 ggtaccagga accgccggcg gggtcaacga ccttgtccag attgcattcg cccagcagaa
     1981 tcagcgggca gttgcgggca atgcggcgag agaattcgtc cggcatgcgc acggcagcgt
     2041 caaagggcag aacgtcaata ctgtccacgc cgcccatgat agcggagaac gcctgggcgg
     2101 agccgcgcag caggttgacc cagggatcca ccttggagag ggtccagaag gaggtgcggg
     2161 catggagctt gattttggca gcttcctcgc tgccgccgca ttcttttacg atgatggccc
     2221 aaagttcgcg cagagcgcgg attttggcga tttccaggaa caggttggcg ccgatggaaa
     2281 ccgtaaagcg catgtgcgcc gcagtttcat ccacggagag gccgcgttcc tccatggcgc
     2341 gcaggtaagc cacggcagtg gacagcatgg cgcccacttc ctcaaaggcg gaagcgccgg
     2401 agtccgcgta aggcagcccg ctgacgccga tggtctggaa gccgggagcg ttggcaacag
     2461 cccatttggt catcacggcc atctggtcat agtaggcgca gatgcccttc cccccgcaaa
     2521 gggagccctt catcacagct ttccccaccg ggtcatacag aacgccgccc ttgaggtccg
     2581 ccgtcttgac accggcggcc ttgcaggtgt tcaggaacat ggagagggtt cccaggccgc
     2641 aggaaccggg agtgacatat acgggaagcc cgtccagccg gacgcccctg agggccgcat
     2701 cccagtcagc ctgcttgcgc agcttcaggc cgcatttgca gttgagctgg acgtttacgg
     2761 cggtcaggcc gcggtccagg tcatgcagaa gcttggcgtt gaagtcttcc ggagaggaag
     2821 cgggaatgct ctgggcgatt tcgcagggct tttccatata gcccagggcg cgcgttccgc
     2881 ggcggtacgg aaattgcccg gcaggcatta ccggggaatg ttcatcccat ttgttataga
     2941 tgggatgcag ggtaattccc tccacaagct tggtaaaaag cttcttgtca aaatcagccc
     3001 ctttcagggc agcgactgct gcctcgcgcc attcggcgta tgtggctggt gcgaattctc
     3061 cgaaatcaat ttccggagcc gttgttttat ttccctgttg catggcgaag tcaatgggta
     3121 aggcctaata tgttggtttt taactatgcg caaaccaggc caagtttaaa gcgcaacatt
     3181 attttaggaa atcaaagtgt aaagtccagc gagaaaaata ctcccgtccg cccgcaaatg
     3241 cgaatttgct tcatcacgcg aggcaaactt gcaaaaacga tatccatggc gtaaatacgg
     3301 gaataagcat gcccccttct actaccgtca aactccctcc ccagctggtc cggcagctcc
     3361 gaactttccg gcgccgcctc agaacggtga agatgctgga agccgtatgc cttgcaggca
     3421 ttgccgtcat tctttcctac gcgctcctgt acctttccga cagattgtgg gagacgcccc
     3481 ccgccgtcgg ctggctcctg tttttcatcg ccgtgtccgg cctggcggtg tttattccct
     3541 ggtggagttt ccggtgggtg tggcagaggc gcacggaatc ccagctggcg cggctgattt
     3601 cccggacgga cgcggccctg ggggatcgtc tcctgggcgt catcgaactg gattccgaaa
     3661 agcacggccg ccaatacggt tccgaaaagc tgaaagaggc ggccatggag caggtggccc
     3721 gcgaagtgtc cgcccgggac ctgacggcga acatcccccg gcccagccac agaaagctgt
     3781 tcgtcctgct gacggttctc gccgcatgca cggcggccgt atgtgcggta agcccggaag
     3841 cggcagggaa tgccctgaaa cgctgggtcc gcccgttcaa tcctccggaa cgctatacgt
     3901 tcacccagct ggcccccacc ccggattccc tggtcattcc cctaggagag agctgcctgt
     3961 atgagatccg gctggcggag ggaaccaaaa cccgcccgca gacggcagag tattttttcc
     4021 gcaacagggt gagccagcag gcgcctcttg cggacggcac atacaagatc cacatccccc
     4081 ccatgcaaca ggcggacaac ctggagtttt ttgcagggga tgccgtgcga cggctgaaca
     4141 tcatcccgaa ggggcgccct tccctgctgg gagccgaagc cggcgtggcc tatcctgcct
     4201 atatggcgcg cgccaacggc agggaagcca tgagagcagg agttatttcc gccccggaag
     4261 gcgctacgct gacgttgaag gtgtccgcct cccagcgcct gcagtctgcg gaggacgcgc
     4321 agggccagcc gctccgggtg gacggaagca gcgtcatcat tccgaacatc cttttggaga
     4381 aagagcccag ggaaatagag cttgactgga tggacagcga cgggctggct gcttcccggc
     4441 ccgtgaagat acgactggaa cccattgaag acaaacagcc ttcaatttac ctgagaggcg
     4501 gcgaaaacga ccgctacgtg ctggaggata ccagcattga actggaagta gaggcggcgg
     4561 acgatttcgg cctccgggaa atgggggtgg aatggcaggg tgaaaaatcc ttttacgacg
     4621 gcaatgagga tgaaaacggg gcctcagctc caaaaccggc cggtgcggac aaaccggcgc
     4681 ccggcctccg cggggagaaa gtgctggcgg acggacagcc cacgcagacc gggctgaaag
     4741 gaacattcct gttccaggcc aaggcgctga agttatctcc ccagcgcgtc gtcatccgcg
     4801 ggtttacgca ggattacaaa ccgggaggca ggcgcgttta ttcggaaccc atgattattt
     4861 tcgtcctttc caagtccgag cacgcccaga tgatccgcaa tgaactggaa cgcatcacca
     4921 gcgagctgga aggcatgatc cgccgcatgg acgccatggc ggacgaggca aaacggctga
     4981 aaggaatgga aagcggcaaa ttgaaagatg cggagtccca ggaacgcctg cacgccctgg
     5041 ccgatgaaga gcaggccaac aggcgcgagt tgggagactt gctcaaccgc tcggaaagcc
     5101 tgtttaagga agcctcccgc aattcccaga tagacccggc cggcatgaag gaattcatga
     5161 aaggaatttc catgctcaaa cccattcccg acggacccat gaaaatggcc cagaaacaat
     5221 tccgggattc cgcctccgag agccgctccg agcaggaaag caggaaggac ctgaacgacg
     5281 gggagaattc ccattccgaa gccacacagg cgctgaaaga caccatgaac cagctctcca
     5341 agtctgcgca ggacatggag gccagcacat tcgtggcacg gctcaggcag gccgcatcca
     5401 aggaggattc tatcgcccat gcactggccg gccagatcaa caccattgcg ggcatgacca
     5461 tggaggagct tgacccttcc accaggcgtg aaatggaaac catcgccacc cttcaaaacg
     5521 cctccacgca ggacataggc tggattctgg aagacctcag ctattacaag tcacgcaccg
     5581 gggagcgcat ttacagcgac ctgtacaacc agatgaacgc tttctccctc cgggataagc
     5641 tggacctggt gcacggcaat atcctgaacg caatcacggc ccgctccatt gacgagtccc
     5701 gcctgtatgc ctccac
//
LOCUS       DAWEMX010000151         5372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5857_length_5372_cov_10.629302, whole genome shotgun sequence.
ACCESSION   DAWEMX010000151 DAWEMX010000000
VERSION     DAWEMX010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_5372_cov_10.629302"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1269 a         1338 c         1503 g         1262 t
ORIGIN      
        1 ccctgaacca tggtgggggc aatcctgttg taacggcgtt ccccgttgcg gcgcgcaatg
       61 atgatttgtg cgccaaagcc gaagccgaat gccagcatga acatggccat gtaatacacg
      121 ccgcccagag cgcatgctcc cagcgccacg tcccccacgc gccccaggaa gacggcatcc
      181 gtcaggccga tcatatgctc aatgatcagg ctgatcatga gggggtatga catgagccat
      241 atcttttgat aggaagtaaa agaaggatta ttcatagcaa gggcagcctg cgggcatggt
      301 ccgccaaggg caggcgctaa aggccaaata tttttgaatt aaaatgtttt cttgcaagaa
      361 agaaatgcgt ttgcttccgc acatgtcatg aaaatggggg taatgccccg ggtcagctga
      421 gcggcagtct ggtttccgga taccgcaggg gggatttggt tggttgtttc atgaaggcca
      481 gcacgaaagc caccatgccc acacggccca caatcatgtt gaacacgatg atccatttgc
      541 cggcatcact gagttccgga gtgacgttcc atgaaaagcc taccgtggcg aaagagctga
      601 ctacttcgaa aaacaggcgt tccagactca actgcggttc tgcaaaacac atgaccgtag
      661 tcatcatacc tacccaggct ccggccagga tgacggtgat gacggcccgt tccaccacat
      721 tgcgggcaat ccggcgcttg tgcatgacca cgtctttctt gccctgtaaa atgcgcgcca
      781 cctccccgca ggaaacggca aatgcggtgg tatggacccc tcccgtggtg gaaccgggat
      841 tgcctccgat gaacatgaga gctcccagaa acagggcgta tggagcgctg ttgagcgcca
      901 tgtccgaaat gttgaatcct gctgtgcggg ccgttgcatt gaacaggctc tcccacaaaa
      961 tccagtacca cggatcggca gacgtaaaca ggctgccgtc aattatttta atcaggaaca
     1021 ggatcagccc gccgccaagc accagagcgg cggtcatgcg tacgaccagc cagctgtatg
     1081 tggaccagtg gcggctgacg gtgttccggg catcccagcc cagtgcgact ctggcccggc
     1141 ggacgatttc cagatacatg gcgaatccca ggctgccgca aagcaccatg accatgatgg
     1201 taatctggcc gcccatattg taggctacgc ccggtgtggc caggttatcc gcaaacaggc
     1261 tgaatcccgc attgcagaaa ccggaaacgg aatggaagac ggcgtaccac cagagcgtgt
     1321 ctccctccag ccccggaaca tgtctccagg aaaaatagag gcctatggcg cccagcgctt
     1381 ccactccgaa cgtgacggca ataatattct tgatgaaccg gtctacctgg gcaatgcctt
     1441 cttcatccag caggttgctg atggttacct tggaacgcag gctcaacccc tggccaacca
     1501 tcagggagac gaaataagtg aacgtcatga tgccgaaagc tcccacttgg aaaagcacca
     1561 gcaaaatggt ctgtccgtaa ttggtgaagt gttcatgaac attcaggggg accagccccg
     1621 tgacgcagac ggcgctggtg ctgatgaaat aggcgtccgt ggcggaaagg acgatgccgt
     1681 ccttggtagc tcccggcgac atgagaagag ttcctcccag cgcaatgaag gcaatcaggg
     1741 taagcaggaa aagaagggcg ggacgaatgc gcgggaggtg gaagcccctg ttccggcggg
     1801 cgatgcggaa aaaatattcc ttgaggttca gcagaggggc ggctccgtag cagaggcaga
     1861 gcgcgcggaa aatgttctga agctctattt tggagataaa ggtggaatgg aaaaagtcgc
     1921 ctgccagcat gaccagaatc accagtccca gccccagttc tacccaccgg gggttgcgga
     1981 tggccctggc aagccatttt tcccgcaaaa tgccgatgac gcccgtgagc tggcccagca
     2041 ccaggaagga gagaatttcg cggaaatagc gcagggaaag ctggtcttca tgaatttccc
     2101 cgaagccgtt ccaccacagc agccacatca atgcggccag tgtgcacagg ccgaacccca
     2161 tttccatgac ctcaaaacgg ttcaatagcg gtttttcatt cgtctcattc acaagcctgc
     2221 aatggtgatg cctctgatgc tggggggaga ctactgcata cggaaagatt tgagaagccc
     2281 ttatatcgtt tccaggcggc cccggctgga ggcaggaagg cgcctttggg aaaaattttt
     2341 ttcatgtgaa ggaaaatagt tgccaatccg ccgccgccgt gcataataag ccccgttctt
     2401 accaaaccat gtgcgctcgt ggcggaattg gtagacgcgc tagattcagg ttctagtggg
     2461 ggttcccccg tgaaggttcg agtcctttcg ggcgcaccaa acaaggccct gcaagttaac
     2521 tgcttgcagg gccttactgg ttttatgatg tattcgtgca ttggctttga cgcaacgccg
     2581 gaagattggt gaaggggctg gataaggatt attgctccgt tgttaaggaa cgcctgtggt
     2641 tttcagggga gggggcgttg cctttttggg agtggatgcc tgcgaattgt tcatgcgcca
     2701 gcttcatgaa catacaggag gaacatttcc tttcagcggt aatgcgttgt ctgcgctcct
     2761 ggtcgtaaag tttctcgtag cgttcggcgc gtttcatttc cctccagagg aagatgccca
     2821 tcacggctgc cacggaggca ccgttttgaa cgtattcaag caggggattg agttctgaag
     2881 tcgccatgca aacaaccgca agggtgttgg ctaaaacgac acttgtagta acggcagtct
     2941 gggtcatggg gctggaaagg tcatgtttcg aaacttctgc gcgcttcgga aatgcttccg
     3001 aattgatgga gctgcaaatg cggcggatct gtaaaggatt tccatgttcc tccccactcc
     3061 aggccgtcaa tttttgcgta aagtttgcca agagtggtgt aaagagggtg attgggcagg
     3121 tagttgcggt cctggaaaat tccaaaatcc caggccaggc cgaagttgtg ccagctctgg
     3181 ccgccgcggg cattcgttac tttagggcgt tgcctgtaga ggtcattctg ttcggcatac
     3241 gtgcgcgttc cgcagataat ttttgcttca tatccctggt tggcggcttc attccgggcc
     3301 agtttgaacc actgccgggc ggctttttgc gcttccggaa gaagggtgga gatgttccgt
     3361 tcggtgcgtt cgtcaaagca tccgatctcc attttgtacc ggtcggcgtc cgtgctccag
     3421 cgttccgttc cgttccgcgt gccgttaccg ggaatgccgt caatggctcc gtgatagtac
     3481 ccggccaggc gcaagagcct ttgccagtag aaggtatctt tctgcaggtc ggaaatgctg
     3541 tcagccattg tcgtggtttt cctgttcctt tttccggtct ttggtgacaa tgccgatgag
     3601 gcctgccgcc gccatgcctg cggcgataat ctggtcggca agagcttgtt cgatagtggc
     3661 tccgcaggct gtggccaggg caaggaggcc cagccatgtg gaacgttccg tcagtcgatt
     3721 gagaatgtat ttgaacatgt gatggtcggt tggtattgaa aagtccctct gtgggagaag
     3781 ggggaacccg cctttccggg aatgccaaag gatggggaaa aggtttttcc gaatcctctc
     3841 cgccaatggg gaaagggggc tatcggttct ccgcacaagt tgctctgagg ttgctgccgc
     3901 gatttgcaaa gattccgcgg atacttttat tgcttgccct aatcggcaaa atccgtcaca
     3961 ggaaacgata cttgtctttt attcatgaag ataccataat gcgccaacca acgccattat
     4021 gaataaccgt ttaacatcgt ttatcatccg cagatttcta ccatttctcc gatatgaata
     4081 tctcagggaa ttggaagcgt acctcagaaa agaggtaacc ggttttccta ctgtaattcc
     4141 tcctgaacct ttttccgacc gccaacagct ggaggcatac cgcagggctc tcgggcaaac
     4201 tattatttcc ggcatttgtt accggatgta cggccttaaa aaggagacgg acaggattta
     4261 ccgtattttc caccttttgc aaacgtgcag ttccggtgaa gctactgcgg atttgcggga
     4321 atacgccgaa tcgttgggcg tttccgtcaa cagagatttt tccaatggaa ttgaactcgc
     4381 cgttctgcat tttctggacg gcaatatcag cttgctcagg cagattgagg aattgcattc
     4441 cattaaaaag aggaaatcgt atcgtattct tccccccatg aaagatgcgc ttcctttccc
     4501 catgattacg gagaaagaaa gggagaagga aaaagaaggc tgccgtcaat atctgtcccg
     4561 aattttgtcg cagaagggga tgactgacgt atgcgttatc agctgtgacc ggatttcctc
     4621 tgattactgg tttaccatca aacgggggca ttatcaggaa accgttgatg aggttgacac
     4681 gtcggccaaa gagatagtca gcagcatacg tactcccggg aatacggaca ttgtcatcta
     4741 cagggggagt tcgaaatgcc tgtacatcaa tttacaggtc agcgccagaa tccaatggat
     4801 gatgcgggcc tatgccgtca ttgctggccg catgctgttc ggctcggata tttgggaatt
     4861 aaggaaccgc tacaccctga gcgtgtttaa taagaagccc atcgccgata ttgaggtgtt
     4921 caataatatt ccgggtctgc gaagggttgc tgtttataag cttgtcatgt ccaggcctct
     4981 ccatgcaagg aagaggacgg agaaagaggt gttgatgagg ggatttacag gcacttacaa
     5041 aagcctttcc gagcttggcg atgaagacgg ccccctgatt gtcgtcccca gcgggttcca
     5101 tgtggacaag gcgttttttt attttgagtt tgaagaagga gacccgaaaa ccgtttcttt
     5161 gactcccgat tccaatggcc tggaattgga aagcgagcaa tacaggctca ttgatatgta
     5221 ttttgaacag gctggttttg accagttgta caataggatt catggagctt aattacggca
     5281 atatcctcaa tatcattatt gaaagtgaga gcctggagtc tcccgattgg atatggaagg
     5341 ccagggtggg cggggatttt tatccggggc ac
//
LOCUS       DAWEMX010000152         5310 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5938_length_5310_cov_10.613892, whole genome shotgun sequence.
ACCESSION   DAWEMX010000152 DAWEMX010000000
VERSION     DAWEMX010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5938_length_5310_cov_10.613892"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    468..477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1088 a         1302 c         1534 g         1376 t
ORIGIN      
        1 ggctccggac agaagcgcag aatataagag aggaacaaga aaagccttca tcctgttctt
       61 ttaaggcagg atgaaatcca cggcaaggaa aaaggggaaa ctgaaaaaag agccttattc
      121 cgctgtgatg ccgccttttt catcgtccag aatgcctgac ttttccagat agtagtcata
      181 tccacctgcg taggcggtga tggtcccccg gttcacatgc agggtttttt gtgccaggga
      241 acggatgaag tgcacatcgt gggagatgaa gaccagtgtt ccttcataat gcttcagagc
      301 ctgcaccagg gcttctacgg acaaaaggtc caggtgggtg gtcggctcgt ccatcagcag
      361 gaggttgggc ggatccacca ggaatttgac caggctgagc cgcgattttt ccccgccgga
      421 aagaacgctg acgcgttttt ccacatccag cctccggaac aggaaggnnn nnnnnnnaac
      481 ccaggatgga acgcgcgtct tcctcccgga tttcagcgct gcatttcatg atttcctcca
      541 gcacggtatt ttccggattc aggttttccg ttcgcgcctg ggagaagtag ccgatgcgcg
      601 tcgtggtacc gggagtgcgt tgtccggagt caatggggat gatgcccgcc aggattttga
      661 gaagagtgga tttgcccgcg ccgttcgggc ctacaagaac agtgcgctcc ccgcgttcca
      721 ccagcaggtc caggttttca taaaccctgc ggtcgccata ggattggcag actttttcca
      781 gctcaatgac tttctgggtg ctgcgcggag gctgggggaa gttgaattta aatactttcc
      841 tgcgggctac cggtttttga atgattttca tcttttccag ctgcttgatg cggctctgga
      901 cttgcccggc tttggaattg atggagcgga aacggtcaat aaattcctga atgtgcgcta
      961 tttccttctg ctggttacgg taggccgcct gaagctgctc gaagcgcacg tccttttgtt
     1021 ggaggaagtc cgagtaattg ccgcggtatt ccaccagttc ttcgttgtcg atgtcgtaca
     1081 cgctttccac cagctcatcc atgaaatccc ggtcgtggga aatcatcagg atggctccgg
     1141 ggtagttttt caggtatcgc tggagccaca gcagggacag gaggtccagg tggttggtag
     1201 gttcgtccag catcaggagg tccggctcca ggacaaggag cttggccagg tacgcgcgca
     1261 tgatccagcc gccggacatt tcccgggcgg ggcgatggaa gtcgctttcc cgaaaggcca
     1321 gccctttgag aattttcttg gctttgggct ccagctggta accgttggcg gcgttgaaag
     1381 tgtcaatggc ttccgcgtat tccggcgtat ccgttttgtt ggcgttttcc gccgtccgga
     1441 tggtgtggat ggcctgttcc atttccggcg tgatgcccag ggcgatttcc agaacggttt
     1501 catccttcgg ttcgctggct tcctggggaa gatagcccac gatggcgtat tcatccatgc
     1561 tgatggagcc ttcgtccggc gtgtcttctc ccagaatcat gcggaacagg gtggatttgc
     1621 ctgctccgtt ggggccaacc agagccaccc gttcccccca gttaatggtc atttccgttt
     1681 ccctgaacaa ggtccgtccg gcgtgggatt ttgtgagatt tttgatcgtc agcatggtaa
     1741 agagagaatg tattctaccg ggatggaaag gggaaggcaa gagccgtcag ggcggaggca
     1801 ggcatttttg aaagctttac tggattttta cgcgtagtct gctagccttg gcgcgccatg
     1861 ccgaccacgc agacattcag gcttttgctt gctgccgccc ttttgtgcgg atggagttcc
     1921 tgctgtactc ctccggcgga ggattatata caaaaacctt acgtgcagca gcaaatgtcc
     1981 gggctggcta atgccttcct ggcgctgctg ccgccggatc aggccgctct tcccgctgcc
     2041 ggagcggagg ccaggtggct ggcggacacg gccgtggtcc agtccgccgc gattgcccgg
     2101 gacaacagga ctgtgctgtt tggctggctg aacaatattc tggttaattc caacctgcgc
     2161 gacagggggc tgtgctggca ggtccagcag gatttgtacc gggatctgcg ccgccgcccc
     2221 gtgaagtatt tccgcatagg gctgacgatt cgagaccgtg gaacgggcag ggagcacagt
     2281 tgcgtgtatg tcaatgccgc ggggaaggga ttgcagggct ccattgtttt ggatgcctgg
     2341 aagaactgcg ggcatctggt gacgctgacc cagaaggacc gggaaggcgg caaatgggag
     2401 gaggactggc gggaaccatt tgtttccaag gcctttccgg agggccattc ctacggcatg
     2461 gaacatcacc tggtctggcc cgggtgggcc aggaaacgcc ccatgctggt tcagcggata
     2521 ttccagtccg atcagcagaa tgaggcgaag gcggccgctg aataaagggg aagcctgatc
     2581 cggcaggaac cggcacacgg tgctgcgccg cggcggtttg cggtatccgc cgtttttaag
     2641 gaaaatcccg gcgaatcggc aaatggcgga gggaatccgg actcagggaa aataaaaacg
     2701 cactcagtgc gtacaccgag tgcggattaa ttggataatt tgctagtgtt gcagggcaaa
     2761 accccgctgc aaacttactt gcccttggcc tgcacaaccg gagcgggagc aggagcggtg
     2821 gtttgctgtt gctgctgctg ctgggagcag gaagcgacca ggcaagcggc gccggcgatg
     2881 gcgaggatag cgatgttctt cataattcga attaaataca gtgtttgtta actactggaa
     2941 cctccgcatc cacggaggtt tgctggcacc aatgtaaatg gtggcaagta gaaatcaaga
     3001 gattttcttg ttccatgtca cggcattaca gaaaagagca ggacccccgg tttctgaagt
     3061 cctgctcttt ttgatgcgtt atggaaaacg gattgtttca aaaccgtttt ttgcgcttac
     3121 aggctgctcc caagctcaat gatggagtag tccccgtccg gcctccgata cacgatctgg
     3181 agcacgtctc gccgcgcatt cctgtagagt acgaatggct tatcggaaag ttccagttcc
     3241 atgatggctt cttccttgta aagctttttg agattgtagc tttctctgtg aatgagcagg
     3301 ggttccgggt cttccgggga atctcctggg taatccagca cggaatcgtc atataccgtt
     3361 tcatccagct tgcggatggt ttcctggtga tggggacgga aattcttcat caggcgcgtt
     3421 ttgtgcttgc gcatgcggcg catgattttg gtgatcgtct catcaatgca ggcgtacatg
     3481 tccgtccctt ccgtggacgc gtcaatggtg atatgatccg agcagaacag gattacctgg
     3541 gaaatcttgc ggtcgcgttg cacatcaagc agtactttaa cctccataat gcgcgggaaa
     3601 tccaggcgga tcccctcaat ttttttcgtt acgaattccc gaatcgcgtc tgtgacttcc
     3661 acgtggcgcc ccgtgatcgt aatgggtgtg tttacgttta ccttttgcat tgtcgtacct
     3721 ctttctagtt gatgttaatg cgcggacccc gtccgcacct ttcaagatat acagatttga
     3781 caatgtttgt cgaggaatcc tttctttttt tcgcgtgcgg ggaaaggcgc ttttcctttt
     3841 ccgtttccat atgccggacc tgggcggctc tcctacctgt tgtgagccaa tcaatcgggg
     3901 agcggccgtt tccgtttatc gctttccgtc gtcttcggaa tacgtttgaa cgtctttgtc
     3961 atacaattgc gggtataaaa aaattatttg aaagtacttg acatgctgcg gaaatgcctt
     4021 ataacagttg cgcactcctc tacagatgct ctctaaatcg cgggatggag cagccaggta
     4081 gctcgtcagg ctcataacct gaaggtcgca ggttcaaatc ctgctcccgt aaccatttga
     4141 aaaccctgca agttcattgc ttgcagggtt ttttcgttac ggatggaaaa gcatccggcg
     4201 agtattggga gtctgttgtt tctgggagtt tttgtattcg gggaactggt gcccaagagg
     4261 gttggaatgt gtgctccgga agtaatttcc aaactggcgg ctgtgccgat gaaagccttt
     4321 tcttgtgccg cagctccctt tgtatgggtg ctttccaaaa gcacagtttt cgcatgcaat
     4381 ttgctgggag tcaggaatac gactgacagg gtgacggaag aggaaatccg cctgatgatt
     4441 caggaaaatg cccgggatgg ggatatacag gatgtggaat gtatatttaa tctcggtgac
     4501 cggaatttgg agtccatcat aacttcgcgg gggggggata ttgcatggct ggatgccaaa
     4561 atgacggaac agccgtgcgg tcagggtcag aggtccgtta tccgttttag accttattat
     4621 aaacggataa catgagaaac gtcgccacca atgtggctgg tcgttttttt catgttgttt
     4681 tgtgcttatg atctacggtg tttccctttt gatgctgcgt tgatttcagt aagcttcatt
     4741 ttgtgctttg tctgccgttt tcgattgtct gagaggtttt ctcttttgaa tcatgcggtt
     4801 tcaacggacc gtagaaatag gaggcggtgg ctcccccgtc aatcaggaag tccgcgccgg
     4861 tgatgaaagc ccccttgccg cccatcagta attccgctac atgggctatt tcatccgccg
     4921 taccgggtct tcttgcaggg cacttcgcaa acatgtttct gtaaaaatca cctctgggac
     4981 cgttgaattc atcgattgcc agaggggtga ctacaatgcc cggggaaatg gagttgatgc
     5041 gtgcgccttt ggcaccccat ttgacggctt ctgccatgac acgcttgacg ttgcagcgtt
     5101 tcgccatctg ataggcatgc agagtatcct tgatattccc gggttggagg agttcgaggt
     5161 tcagcaattc ctctgttgga gtcatggcca gttgttcgtc ctgttcggca gtcagagcgg
     5221 gcatgcggtg accggattgg ctggagatag ttactcccga gccgccgggg caaataactt
     5281 tccccacctc ttccagcaat acggcggttc
//
LOCUS       DAWEMX010000153         5271 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5982_length_5271_cov_9.320360, whole genome shotgun sequence.
ACCESSION   DAWEMX010000153 DAWEMX010000000
VERSION     DAWEMX010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5982_length_5271_cov_9.320360"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2628..2637
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1274 a         1193 c         1467 g         1327 t
ORIGIN      
        1 gattactcag ggtacgccga tggggtttga cggtaagatg gaagtgacaa cccttgaggg
       61 agtggaacaa accatttccc aagggcctac cgcggctgtt gtgcccatta aacagctggg
      121 aacgaatggc ggcggttatt tcggcgttaa ttcatcccat ccgctggaaa atccgactta
      181 cctttccaac atggtcgaat gctggtccat cttgattatt ccgatggcga tggttttggc
      241 ctttggtttt tatctcaagc gcaagaaact gggatacagt atttacggcg tgatgttgct
      301 tgccttttta attggcgttt gcattaacgt gtatcaggag atgaacggca atccccgcat
      361 agaagagatg ggcatcgtcc aggaaagcgg ggctatggaa ggtaaggaaa tccggctcgg
      421 atccgcggct acagctttgt ggagcgtcgt tacgacagtt acgtcaaatg ggtccgtcaa
      481 cgggatgcat gattccacca tgcctctttc aggcatgatt cagatgttga acatgcagat
      541 taatacgtgg tttggcggcg tcggggtagg gtggatgaat tatttcgcct tcatgattat
      601 agccgtgttc atcagcggtc tgatggtggg aagaaccccc gaattcctgt gcaagaaagt
      661 agaagcccgt gaaatgaaaa tcgcttccat tgtggcgttg ctccacccgt ttgtgattct
      721 cgtcggaacg gctctggctt gttatctgtt tgtttacgcg ccgtcgtttg tggaaggcga
      781 aggagggtgg gtcaataatc ccggctttca tggattgagt gaatttcttt acgagtatac
      841 ttcaagcgcc gccaataacg gttccgggtt tgaagggctg gccgataata cctggttctg
      901 gaattactca tgcggcattg tcctcattct gagccgtttc atcccgatca tcggccaggt
      961 tgccatcgcc gggcttctgg cgggcaagaa gtatattccc gagacgtccg gaacgttgaa
     1021 gacggattcc gtaacgttca gcgccatgac ttttgccgtg attttcattg ttgcggcttt
     1081 gtccttcttc cctgtccatg ccttaagcac cattgccgaa catctgagtt tgtaaattta
     1141 gggtagaata cgtttaccac caataatata tttacatatt tccgttccag tttctggaat
     1201 tgaaaaagaa atctacgata tgaaaaattc caataatact tccctgttcc aaagggaaca
     1261 ggtacgggaa agcttgaagc agtcattgat caagcttcat ccgggaatga tgatgaggaa
     1321 cccgatcatg tttaccgtgg aaatcgccac gatgatcatg cttctggtaa ccgtttattc
     1381 cctcttcagc gacggacaag gggatttcct ttataatttc ctcgttttcg tcattttgtt
     1441 cctcaccctt ctgttcgcca attttgccga agcgattgcg gaggcccggg gaaaggcgca
     1501 ggccgacagc ctgcgcagga cgcgtgagga aacgccggcc aagttgattg tcgatggcac
     1561 ggttaccgtc atcaatagtt cccagttgaa aaaaggggat tatttcgaat gtgtcgcggg
     1621 agatatcatt cccgccgacg gggaggttgt cgaagggctg gcttccattg acgaaagcgc
     1681 tattacgggg gaatccgctc cggttatccg tgagtccggc ggggataaga gttccgtgac
     1741 gggcggcacc aaagttctgt cagaccgtat caaggtgctt gtcaccgcgc gtcccggaga
     1801 gagtttcctt gataagatga ttgctctggt tgaaggcgct tcccggcaga aaacccctaa
     1861 cgagatagcg ttaaccattt tgttggctgt ctttaccctg gttttccttg tcgtctgcat
     1921 taccttgaag ccggcagccg attattccgg aacggaaatc agcattgccg ccctgatttc
     1981 cctgttcgta tgtctcattc ctacgactat cggcggattg ctttctgcga tcggcattgc
     2041 cggaatgaat cgtgccttga gggccaatgt tattacgaag tcagggaagg ctgtggagac
     2101 ggccggggac atcagcaccc tcctgcttga taaaacgggt acgattacga ttggcaatcg
     2161 gaaagcgacg ggatttttcc ctgtccaggg ttttgacaag caggcttttg ttgaagcctg
     2221 tctgatgtct tccgtttcag acgatactcc ggaagggaaa tcgattatcg agcttggaca
     2281 tgaatacggc gtccatatgc gcaaccttca tacggagggg gcccgcatga ttcagttcac
     2341 ggcggagacg aagtgttcgg gcattaatct gagcgatgga accgaaatcc ggaaaggggc
     2401 ttttgacacc atccgcaaga ttgccgagga agcgggaaat ccattcccca aagaagccga
     2461 ggaaatcatt gaaagagtgg cgggcaatgg cggcactcct cttgtcgtct gcatcaacag
     2521 gaaggttgtc ggcgttattg aattgcagga tatcatcaag ccgggcatag aagagcgctt
     2581 cgaacgcctg cgcaagatgg gagtaaagac ggtaatggtg acgggggnnn nnnnnnntga
     2641 cgggggataa tccgagtacg gccaggtata tcgctgaaaa agcaggcgtt gatgatttca
     2701 tcgcggaagc caagcccgaa gataagttga gttatatcag gaaggagcag gcttccggca
     2761 aactggttgc catgatgggg gacgggacga atgacgctcc ggctctggcg caggccgatg
     2821 tcggcgtagc catgaacagc ggaacccagg ccgccaagga ggccggcaat atggttgacc
     2881 ttgataatga ccccaccaaa ttgattgagg ttgtagaaat aggcaagcag ctgctgatga
     2941 cgcgcggcac gttgacgacg ttttccattg ccaatgacgt cgccaagtac ttcgcgattg
     3001 tgcctgcgct gttcatgatt gcgattccag aacttgctgc gctgaatatc atgcatctgc
     3061 atagtccgaa aagtgccatc ctgtctgcga ttattttcaa tgcaatcatt atccccatcc
     3121 tgattcctct ggccttgagg ggcgtggcgt acaagcccat tggagccagc gctctgctgc
     3181 ttcgcaacat cctgatttac ggagtgggcg gtctgattgc tcccttcatc ggaatcaaac
     3241 tgattgatat ggctgtcggc ctgttttttt aactgaaata aatatattgt tatgagaaat
     3301 ttagtgaaat cattgcgtct tacacttgtc ttttgcgtct ttttctccat atgctacatc
     3361 ctagtgctgt ggatattcgg attgattgcc ggccctggcg gaggaaatgc ggaaaccctg
     3421 gttttgaacg ggcgcgtggt aggcgccgcc aatgtcgggc agcaattcac gaaggatatt
     3481 tatttctggg gccgtccgtc cagttcatcc ggcagcaata aggggccgac caacgaagaa
     3541 tacctggccg acgtgaatat tcggatagat tcgttcttga agaaacatcc ttatttggaa
     3601 cggaaggatg tcccttccga aatggttacg gccagcgctt ccggcctgga tccgcatatc
     3661 actccggaga gcgcctatgt gcaggtgaag cgcgttgcca aggcccgcgg catggaggag
     3721 ggggcggtga ggtctcttgt cgatcaggcc gtggaaaaac cgctgctggg catgtttggc
     3781 acggagaagg tcaatgtgct taagttgaat attgctcttg agaaggctga tccttcaggg
     3841 aagcattaat ttcctgattg tttagggcgg caatggcgat tccggatttg gtgataaatt
     3901 gccgccttga aaaaatgaaa tcaattgcgg gcttcatgtc atggtaacgc ccgcaattga
     3961 ttttcatttt caaaagattc tgtaaaatca acaagatgtt tttaaccgca ggagccgggt
     4021 gaatttaata tgattgacgg aagtaacgga gcagagcact ttcttgacct gatacggcgt
     4081 tctcgaaggg gaaagttcaa gatttacatt ggtatgatgg ccggagtagg caagacgtac
     4141 cgcatgctgc aggaagccca tgaattgctg aataacggca ttgatgtcag aatcggttac
     4201 gttgaaaccc acgggcggcc cgggactgaa gccatgctcc ggggggtgcc cgtcatcccg
     4261 cgtaaagtgg ttttttacaa aggcagggag attgaagaaa tggacctgga cgcgattctt
     4321 caagcccggc ccgagttggt tattgttgac gaactggctc ataccaatgt ggaagggagc
     4381 cgccatcgca agagatggga agacgtgatg gagctgcttg acgccaatat taatgtgatt
     4441 tccgcgatta atatccagca catcgaaagc ctgagcggcg aagtgaagga gattacgggg
     4501 attgagatga aggaacgggt tcccgacagg attttgcagg aggcggacga agtggtgaat
     4561 atcgacctga cgtccgagga attgatcggc cgcctcaagg ccggcaagat ttaccggcct
     4621 gaaaaaatcc agattgccct ggataatttt ttcagaacgg aaaatattct tcagctgcgt
     4681 gaactggcat tgaaggaagt tgctttccga gtggagaaaa aagtggaaac ggaagtattc
     4741 ccggcagttt cttccgccag aagggaaaag attctggcct gcatcagcag caatgaacag
     4801 acgccccggc gcatcatccg aaaagcatac cggatttcct cgcgttacag tacgtcgttt
     4861 atcgcccttt acgtccagac gccaagggag tcccccgacc gcatcaatct ggcatcccag
     4921 cgccatttgc ttaatcattt caagctggtg gccgaactgg gagggacggt gaagcaggtg
     4981 agtgcggaag acatacccca ggcgatcatc gcaacgtgca gggaatatca tattacggct
     5041 atttgcatgg ggcatcccgc attcaaaatg cccggagcca tgttcaagat actgggatat
     5101 aaacaatttc tgcgtgtctt gtcggagatg aagatagatt taatcatcat tgcataaaaa
     5161 acagcggtaa atttgaaaat aaggccggag ccggctcttt tcacaggaag caggcgatga
     5221 gattatcatg cttgtcttcc ctgagcggaa cggattgttg ccggcagttt c
//
LOCUS       DAWEMX010000154         5270 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_5983_length_5270_cov_11.254650, whole genome shotgun sequence.
ACCESSION   DAWEMX010000154 DAWEMX010000000
VERSION     DAWEMX010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5983_length_5270_cov_11.254650"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1148 a         1412 c         1393 g         1317 t
ORIGIN      
        1 gattccaaaa cctccgtggc atccagttcc gcgatttgca ggtagccgtg atagagaacc
       61 gtttcttcga catcaggatc tctctttcat agacggatta ataaattttg ccgactactt
      121 cgtgttaaga acctatcgcg aaccctatga tcatgatgac aagccatgta cctgacaata
      181 taagtgcaat aagatcgtct tttctgtttg aattcacgat gataccacac aatccccaaa
      241 acaggcttgt gaattgaccg aggaacatta ccaatcctaa tgttatagat agagagacag
      301 gaaagcgaat gtttttgtcg aatagtgtta cacaagctat aggaaacagt actatccatc
      361 cgattagtga aggggtgtaa gatttcatga ttgattattt ttaatgcatt cacagatagc
      421 actctgtatt tgactatatc gattctttaa ttcttctgta ttaacgcccg gatccgtcac
      481 gtactgtgga ttaccttttt tgttctttcg aacttttctg aggtaatcct ctaccccctg
      541 tcggaatgca ttcacatgaa tttggtttat ttgtaatttt tttaattgtt gatcagaatt
      601 ctgcggatat acaacaccgt tcgacgcctt tcctaaatca tcaatgacat gactcataat
      661 aagagcagaa aattctatgg ctccttgatt agttcgaata taatctttta cctgtccaaa
      721 cgttgctgaa gagcctaata aactttcccg tccataccga atggcggttg caaagtgaat
      781 attgacagga ccaatatctc ccttaaaaaa gtaagcgtta tcaggaagta tgttttcaat
      841 aatattatct tgcacccaat ctgtcgccca tttaattggc caactttgat aatcatcata
      901 ttcttctgcc atagccattg aaagagcata cggatcaact ttatacaagg aagaatactt
      961 gacaatatgc ttacaatttt ctttgagcca ctcaacggta gagtctttgt ataaaccaaa
     1021 gacatcaaac catgtggatg gtgagttttc agaaaagcaa tatggatttc ttgaaggaat
     1081 aagatctcta ctaatccacc tgccattttg gggattataa taacggtaat tgtagtaaac
     1141 cagccccagt tcgtcatcag cgtattcgct ggagaaccgg aacggattgt cctctgcggc
     1201 attcccttcc atcctgacaa tattcccata cgggccgtat tcgtacaagg cgcggcgccc
     1261 cgctcggatg ccgaagagcg ccgtcgtgtt cttcaatgcg tcatgcgtgt agtaaagatc
     1321 ttccacgtag gctcccgttt catcaaagac gcccatggcc agaatgcgcg tggctgccgg
     1381 ttccagcgga tcccacaggt acgttttacg caggacgggc gccacggatt ccaaaacctc
     1441 cgtggcatcc agttccgcga tttgcaggta gccgtgatag atgaaccgtt tccttgacat
     1501 gagcgattct ccctcataga cggatttttc aacccgcctg ttcagataat cgtatacgca
     1561 ttcaatacgc ctgtccccct gtatgaagct gaccgcctga ttcaacgcgt tgtaggaggc
     1621 ttcccattcc ccggtggcgg tccggagctt cgtctggttg ccgtcggcgt cataattcgg
     1681 cacaaaagga gcctcctcac cttcggtgat atccgtatat tgattgaccc ggttggcaac
     1741 gtatgtagtg agaagggaat ccgttccctc cagggacgtt ttgcggttgc ctatgttatc
     1801 gtaggagtag ctgtatgtgc cgccccggct catcgcatcg ctcacaagtt cattcctgtc
     1861 atcgtaggcg taggtgtgcg tcaggtcggg tgcaggcgcg ttaaagtagt ctttcttcgt
     1921 gaccgggcgc cccagtatgt cgtaagagta gtcggttttg gccgggtagt tggcgctgcc
     1981 ggggcgcagg tagtcaatct tcaccgggag gtcgcgcttt tcttccaaag tgcgccaccg
     2041 tttcagggtg ttggggtaat caagagtgtc cagcaggccg gtttcctcat tgtagccata
     2101 ggtgaagggc ttcccgacgg cgttcagcga aacggaggat agccttccgt aggcgtcata
     2161 ggcccatgcc gtctgcaagg cgggggcgcc cgcataatcc agacagtagc ccgcaggcct
     2221 ccccactccg tcacggcgga gggttaagac gcttgccgca aggccagccg tcgtttccgt
     2281 ttcctgttcg ttatactggt tgtacgtgaa catgcgcgtt ccggaagcgt cgtcaatctg
     2341 cgtcagctgc cccagatgat tgtagaggct gctgatggag ggggtaaaac cgtcgtcatt
     2401 aaaggtaatg gaaacgagct ccccagtaag gggggcgtag gagcgcgaag caacggttcc
     2461 tcgcgcgtcc gtcgtagcgg caacgcggtt gagcgcatcg taaacggtat ccacatgggt
     2521 ggcattggca taggtcttgc ggacaaccag ccccgtagcg acatcgtacg tccaggtggt
     2581 gatgtcccca tccgtccgga ggcggggatc agtagtgatg acatccccgg gatcacggaa
     2641 ggtggaaagg ctcactatat ggccggcggc gtcataagca tatacggcgg gctgtatggc
     2701 cgtcccccat tcggcttcct tgcggccccg gatatcataa acggaacagg ctgttttacc
     2761 aagggcattg gtaacgcagg taggcagatc aaagggctgg gcataagcgt aggtggtcgt
     2821 gttgcccgcg gcatccgtgc cggaaatgat gcggccggcg atgtcgcaga cggcggtaaa
     2881 gacgttgccg cgcacatctg tgtccgcata ggtgatgccc gtttccgtat aagtgcgctg
     2941 ccggcgggcg gtcgcgccgt caaaacccgt aaccgccgtc gtgaatccgt ccacgacgcg
     3001 cgtgagggaa acgctcccga cgccgggcac ggttttcttc tgcagacgga cagaaccgcc
     3061 gccatactcc gtccactcca cggtatcatg gccgcgggga tccgtcatga cggttttgcc
     3121 ggcaagaaca gaactcaaag cagagagcag ggccgcctgc ttttgaagaa cgggagtacc
     3181 tgaagaagta taacttgtta tggaaacaag tctgtagatg ccgtcttcac ggctctcggc
     3241 cacataagcg tagtcagtaa tgcgggagtt caggatggtc ggttcatcct ccagggcaac
     3301 gattgccctg gtcctgttcc caaaggcatc ataggcatag agcgtcggcg ccatgtccgc
     3361 cagctgttcc ctgataaggc gccccttgtc atcgtatgca agcgtgcgca cgttttcccc
     3421 attcagggta ttggcggcag caatccgtac aagatcgcca aagccgttga cggtggagct
     3481 tcccgtcacg acgcggggct ctcccccctc cccgcccggc acagcctccg tcgtgcggat
     3541 cccttccgcc gtggcctcaa cggtataaag gatatcctgc tgcccggtgc cgtaacgccg
     3601 caatattgtt ccggaagcac ttttccgggt gatgagggta gcgccggcgg gggtggtaac
     3661 agtttctacc aggccatccc cgctatagga gcgaacggtg gagcgcccca gaatatcggt
     3721 ttcttttacc agacgcccca gcaaatcgta ttcccggctt tccaccgtcg tcattgggcc
     3781 tgtatccctg cgcagggagg taattttacc gagggcatca cgagtgtaac tcgtgatggt
     3841 ttccggagtg atcatggttt ccccgtctgc aatggctgaa cggatggttt ctgtcagctg
     3901 gcgggccgta ttgtaggaat aggaagtcgt cacgccattc tcatcctttt cccataaagg
     3961 gccgcaacag gtcatcatgc gctcgtaaac gcgtccattg gcgcgggtgc gctttaccca
     4021 acggttctca acatcatatt cataagaaga cgcatccgtc attgaccaaa caccatcggc
     4081 aagctgtacg tattgctcat aacgcatgtt attgccctgg ccggagacgt aagtaacctt
     4141 ccgtgaactc aggccgggca cgcacgcacc cgctatccgc gtttccgcgg tcactttgta
     4201 aagggctccg tactggtccg tctcttcata aaaataatgc gtctgaatgc cgtccatgcc
     4261 ctgtttcatc ttcaggcgtc cgcgggcata aacattggga gccgtgccga gccaggattc
     4321 cttcacttcc agacgcacgt tttcttcccc caatgcggtg gtgcgttttt ccacgcgccg
     4381 cacatgattt tcctcaacgt aggtgtaatc cgcgcgccaa agttccgccg cagtggccgt
     4441 caacgtcacc cattgggtga ggatatccgg atccgcctgc gtctggtccc gatacgtggt
     4501 gtaagtgacc ttatcgccgg cttccgccca ggggacgctg gaaacgattt cacggccgaa
     4561 acggtcgtat gcccacgttt tgacacttcc gtcaggagca gtttccttcg caatttttcc
     4621 catgccggtg tactcgtaag tcgtttcccg ggcgtagtcc gttccgtagc cttcaatacg
     4681 gctggtgcac aaattgccat tgcgggtgac ggcataagtc tcacagacgc gggagatggg
     4741 agcgccgttt tccccccgct ggatggtggt gatgagtttc cacgtgccgg gcgaaacgat
     4801 ctgtttttct ttcagagtga atatggcgtc ttctccttcc ccctgcgcca tgttccatgc
     4861 tccgtcggct ccctgccagt agcgggtggt gtagggcgcg cgcccggcgg tctgttcggt
     4921 aacggagagt ctgcccgcag ccgcgtcccc ggtaattgtg aaggttttaa aggggtcgcc
     4981 cgtgacggaa taaaggccgg tggaactgtc tttcccgccc acctgttcgg ggagatagag
     5041 ggcgatgcgg taaccggact ccgtgacatt ttcgatgttg gcaagcccat cccacaggtt
     5101 ccatatctgc cgaatgcttc catccgaagc ccgcacgata tccagctttc cgtcaaattc
     5161 ttctcttgtg taggacgcgc catttttagc ggtgtagccg gtggcgtatt tcaaggaaga
     5221 ggcagcacct gcggtatagt taacagtatt acccgcggtt gaacggatac
//
LOCUS       DAWEMX010000155         5124 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6176_length_5124_cov_10.495956, whole genome shotgun sequence.
ACCESSION   DAWEMX010000155 DAWEMX010000000
VERSION     DAWEMX010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6176_length_5124_cov_10.495956"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3786..3795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1138 a         1515 c         1394 g         1067 t
ORIGIN      
        1 tatttcctgg ctcccacttc atccggatcc tccttcagat cattcaggga gaatttaata
       61 cggaataatc tcatgatgaa cacataagaa catggaatca gaaaagcgcc caccagtgta
      121 gccaccccca tgccaatcac caccaccgta ccgatcgcgt tgcgcgccag agctccggaa
      181 ccggaggaaa gcatcagggg gatgcatccc agaacgaatg ccagagaagt catcaaaatg
      241 ggacgcaggc gcagacgcgc ggcagccagc gtagcctcca tcaggccctt gccgcgctgc
      301 atctccagat tggcgaattc cacgatgagg atggcgttct tggccgccag acccaccagc
      361 atcaccagac cgatctgcgc gtagagcgtc agctccatgc cctgccagaa caaccctgca
      421 taggccccca gcacggcaat gggaaccgtg aggaaaaccg ccaggggaag agaccatttc
      481 tcataaagcg ccaccaggat aaggaaggcg aaaacggcgg acatggtgaa gatggccccc
      541 agcccaatac tgttgcgcac tttattttcc tggaagctca tgtcctggta gtcgaagcca
      601 accttcgtag gatccatcgt ctgatggaac acttcctcca gcgcgtccat cacctgctgg
      661 gtggagtggc cgggccgggg catcacgttc aatttggcgg cgttgtagat gttatggtgc
      721 atcacgaatt ccgtatcctc tatctctctg atgtttacca gggcggagag aggcacgcgg
      781 gctccgtccg cgttcgtgac aaagaagccg tccatcttct ccaggctggc gcggtcttcg
      841 ccctgcgcct ggatatacac ctgccactgc tggccgaagg cattgaagta attgatgaag
      901 gaggaaccgt tgtaggaggc cagaatgccg ttggccacgt caatatccac cttgtggaat
      961 ttgcattttt ccttgtccag gttcacgtac ttctgcggca tgtccgccat catcatgtct
     1021 atgcacaagg ctatttccgg acgccgggaa gccgcttcct ggaaccgttg cacctgttca
     1081 tgcagataag ctacgccctg cccttccaga tcctccagta ccatcgtaac cccgttggcg
     1141 gttcccacgc cggggatggc gggaggttcc agcaccatcg ccatgccgtc caggccggcc
     1201 tgggccagct ttccctgaag ctttccggcc agctccgcgg aggtttccgg ccgttcgctc
     1261 cagtccttga gctcaatgta ggcaatgcag gcgccgggag tctgcacgct gttgaggatg
     1321 ttgagcccca ccacggtagt aaggttcttc acggcgggat cctggagggc cgcctcaaat
     1381 tttttgtcct gttcataggc aacctgcagg gaggtattcg gcttcaggat caggcaggcc
     1441 agcagaaagc cctggtcctc atccggaaga aagccgccgg gaacgcgctc cgccacagga
     1501 gccaccgccc cccagaataa caacaggagg gggatggaaa tcgccagctt gcgggccagg
     1561 cttccgcaca tgcgggtata gccgttggag acacgtccgt aacaccagtt gaacaaacgg
     1621 tgaaatgggg aaaagaggct cttcctgccc gcattccggg ggttcagcaa cagggcgcac
     1681 aaggcagggg aaagcgtcag cgcattaaag gcggaaatga gcatggaaat ggcgatggtc
     1741 acggcgaact gcttgaacag cgttcccgtg atgccctcca gcagcaggga aggcaggaat
     1801 accgccgcca gcaccagggc aatggcgatg acggggccgg atacctcctg catggcggcg
     1861 aaggaagcca tgcgcggatt gagccccttg tctatgtgct cctgcacggc ttccaccacc
     1921 acaatggcgt catccaccac caggccgatg gccagcacca tgccaagaag ggaaatggtg
     1981 ttcagagaaa aacccaggac aggaaagagg cagaacgtgg aaataaggga aaccggaacg
     2041 gcgatgagcg gaatgagggt ggcgcgccag ttttgaagga aaacatacac gaccaaggcc
     2101 accaggatga cggcttccat gaacgtgtgc ttgatttcat caatggaata gcgcaccgcc
     2161 gtagtcgtat ccagggaaac gcgcccggta attccttccg gcatcagcgg agccttggct
     2221 tccagcagtt tttccacgcg gtccgccgta gcgatggcgt tggaacccgg agactggaag
     2281 ataacgatag ccgcggaaac ttctccgttc aggcggccgg tggcggaata gtcttcagct
     2341 cccagttcca ccttggcaat atctttcagg tacaggattt catccccatt ctggcgaatg
     2401 atgatgtctt caaattcatg gggctccgaa agacgcccct gagtgcgcac ggtaatggtc
     2461 tgctcctggc cgtccggcac ggggtccgcc ccgatcttgc cgccggggtt ggtggtgttc
     2521 tgggcggaga tggcgccccg cacttcattg acggaaacac cgtaatgggt cattttggac
     2581 gtatccagcc agatgcggat ggcataacgc cccgcgccgt acacctgaac ttcccccacg
     2641 ccctccacgc gcttgagttc atccaccagc ttcacctggg catagttcgc caaatccacg
     2701 gaattataac tgccgtccgg ggatgtcagg gaataaatca tcagcggaag ccccggctgc
     2761 tgcttgatgg tcacgcccgt gctctgaact tcctgcggaa gctgggagga ggcctgccca
     2821 tagcgcatat tggtcagcac ctggtcaata tccgtatcgg aacccggttc aaaaatgacg
     2881 gaaaggttat acacgccgtt gttggaggaa acggacgtca tgtagtccat gccctgcacg
     2941 ccggaaatct ggcgttccag gggagtgccc acggaatccg ccaccgcctg cgcgctggcc
     3001 ccaggaaaca tggcctgaag ctggatggtg cgcggcgtga tttccggata ctgggaaata
     3061 ggcaggttga acatgcagac cagccccagc agcgtggtca caatggcaat cacggctgcg
     3121 attgcgggat gcttgatgaa aaacgcactc atggcttatt tggcagttac ggaaggagtg
     3181 gtggaaacag gggcggtata gatgaaggga acggggacaa ccgtcccgaa cccttccgtg
     3241 gctccatgtt cccgcatcag gctgttcgcc ttggaatact gggcggccat cattccgcct
     3301 tccacgatga cgggagcatc cgccggcttt ccgtatccta tttccttcag agattccgga
     3361 atgggcttga ccgttcccgt cactatctgc atcagcatgg gggccgttcc gcccttgccg
     3421 tcaggcatgt caagaactac ggtttccccc ggctgcacgt caatgcggcg cggcatcccg
     3481 tccggagcca ccaccatgat gttgcggtgg ttcatggaag aaagcagggc gcgggcggga
     3541 accagcaagg catccttctc cgtacccgtc acggcgcgca cgcgcaccgc cattccggaa
     3601 cgcagtttca gatccggatt gggaatatgg ccgatgaaat tgacggtgcc cgtcgaacgg
     3661 ctcacctcgc tgtccacgga aaccaccttc cccttctttt caaagacgga gccgtcttcc
     3721 agaatcaatt caaaatccgg catgtctccc atgcgcccgg ttttcacatt cacggttcca
     3781 tccatnnnnn nnnnngctgg ccgtcgcttt ggaagccagt ccccggaacg gcgcgcgtac
     3841 tttggtgcgg tccagattga tccgggcgtt ttccagggag gcttccgcct gcttgatgcc
     3901 cgcttccgcc tgttccagag cagcctgcat ctccttaagg ttctgttgcg cgtccgtcag
     3961 gttcttgacg gaaatagccc ccgtcctgat cagtttgtca tagcggtcca aatccttctg
     4021 caccctgttc agagaagctt ccgcctgaag tttgacggca cgagccgtag ccaggttggc
     4081 cgcctccctg tccacggcgg cctgataggt agccgggtca atttcaaaga gcagttctcc
     4141 cttgttgcaa atggagccgt cccagtaaac ctggcgtatc agcgttccgg aaacttgcgg
     4201 cctgatgtcc gcctgctcca ccccgcgcaa atggccgaac caggaagatt tcaactccac
     4261 cgttctctgc tccatattca tcacggaaac cttgagcgga ggcatttccc tccggtttgc
     4321 agcatccgcg gcgccgttgt cttcccggca ccctgtcagc ccgcacaggg ccgctatcag
     4381 tatatagtgt gctttcatgt tgttggttaa caatattatt gaagcatgct tcatattttt
     4441 ccaaacccgt tccccggaaa aaagcacggg cttttattat ttgagattgt cgtacatgcg
     4501 ccggagcaga tccctcagca tgctgcggct ttcctccggc accccgtcca gcagcgtatt
     4561 ttcaatcact cctatctgct gacagatggc ctcatgaacg ccccggctca tatccgtcag
     4621 gtacacattc ttcagccgtc cgtcttcact gtcaaaagcc gtggttaccc agcccttctg
     4681 ttccagcctc tggacaattc ccttggccgt ggtatgggag acacccaaat gccgctctat
     4741 gtccctctgg gagacgtttt ctccgtcacg ggacatcagg taagccagca cccggccctg
     4801 ggaggccgta acacgctcca gagtcaagcc gcgcgtaagc atcatatcca cgcggtccgt
     4861 aatcattttc atcaaaaacg aaatcgtaaa agggtcggcc ataaaacaat gaagcatgct
     4921 tcaaaaatgt accgataggt actctttatc cggacgcaag tcaagcggaa tcagaatgac
     4981 agagcgcggg aaaaacacgt tgacctcacc agtaaaaata aatcaaatcc gttctttatc
     5041 aataaattga aatatcaaat aatcaatact cggtatccgt tctaatgtct ttcccgcatc
     5101 atccggggag gccgggcaaa cgta
//
LOCUS       DAWEMX010000156         5071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6233_length_5071_cov_11.420455, whole genome shotgun sequence.
ACCESSION   DAWEMX010000156 DAWEMX010000000
VERSION     DAWEMX010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_5071_cov_11.420455"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1323 a         1453 c         1307 g          988 t
ORIGIN      
        1 caacatggca aaagcaaata atttcatgat aatctgtaca tttggatgtt ttttgcatat
       61 caaacgcgct ccttttaatg tacgaatttg gaatccgttg ttattcgaaa ctcaaaacaa
      121 caaaataaca atcaatgaat tattgttaga aaaaaagatt gaaacagaac aagattcctt
      181 cctcttctca caggttggcc attgaatata aaaaaataat ctttactccg gattccaaat
      241 tcgcaaccat actgaaaata aaatggtttg atagttgatg ttttatattc ttctttaaat
      301 aaatcaaatt ttcaacaata gagccacaca gtcccaaaaa ctgcgggaat gcagttacaa
      361 tcaatccatt cttgcctccc tgccggaaaa atcccatact ttctgcatga catggagttg
      421 gaacaaactt tccgccgcca aatgggaaga cgcctggagc gaacgcatcg ccggaaatcc
      481 gaacgccgtt attacaaaaa tcaaaggggg gaaaacggtt cgaatcaccg tttactgcga
      541 tgatgaagaa gacgccctgg ccttgaaaaa atattttgga ggaactgtca gggaaatcaa
      601 gacgcaggac tgggtagcga cccagaagcg ggaaaaacgc cctccgctca aaataaggga
      661 cgcactggtc attacggagc agagttctcc ggagaatctg gccgctctgc ggaaacagtt
      721 cccctcgcgc tgcatcctga gcgtccccgc cgaaatggct ttcggaacag gagaccacgc
      781 taccacatcc acctgcctgc gttttatctg cgacgctgcc aaatcccgca gaaaaacatc
      841 atggagcatg acggacatag gctgcggaac cgccgtgttg gctatggcgg cgctgaagct
      901 gggggcggag cacgccacgg ctttcgattt tgaccccatg gccattgaag tggcccggca
      961 caacatggtc cgcaacgccg ttacggcgga acagctggac ttatttgtgg aagacgtctt
     1021 caaatggaaa ccttcggaat cccagcaggg tgaccttgtc gtcgccaacc tgttttcaac
     1081 catactgcaa aaggcttttc cacaaatcat cgccgccatg aagaaggatg ccgctttggt
     1141 catctccggc attctggctt cccagtggga agaaacgaaa acggcagcgg aacgtcacgg
     1201 tctgttcttt gaacgggccg tcaaacgcgg gaaatggatt acggcaaagg gaggaatggc
     1261 ccggtagcaa atagactcgc tccgcatttt tgttccgccc tcacggccct tccttcatcg
     1321 gaacagcaat acccggcttc cagttcaaaa atctccgcat cccggcaccc ctgctacaga
     1381 aaacaggaat gcactttcat tgtccggcag gaaaaaccag cccgcatgcc cggcatgtga
     1441 ttctccttac gggcaggact atccctccgc acgttgttca cgggcagaaa acagtctctc
     1501 tacatacttg gccagcatat ccacttccag attgaccggc tggccgggaa cggaattctg
     1561 cagattcgtg cggccgaaag tatggggcgt aatccagaac tccagcagag agccgctcaa
     1621 attggctatg gtcagggaaa tgccgtcaat ggtgatggaa cccttgtcaa tacaatagcg
     1681 ggtgagttcc tccggaatgc gcacctccaa acggtggtcc tgccctacag gctccagagc
     1741 gacaatcgta ccggtatggt ccacatgccc catcacgaag tggcctccga aacggtccat
     1801 ggcggacatg gcgcgttcca gattaaccag catccccggc ttgagggttc ccagactggt
     1861 gacgcggaga gtctgggaaa gcagatcaaa ggagatgcgt tcaccctcca gggcatccac
     1921 cgtcaggcag catccgttga cggcaacgga atcccccagg gaaagttcac ggccgaacgg
     1981 aatggtcagc gtcagcctgg ctccgccgtt aataggcgta aacccttcca cggttcctgt
     2041 tgtttcaacg agtccggtaa acataagagt gacagtacgc tccggccatt aatggccgaa
     2101 gcaggtgaag aaaataaaca cgatggcagt gggaaaagcc gcccaggcaa acagccacag
     2161 gggattgatg cctttgaaat atttacccag aatggcctgc ccggccggat tgggggcgtt
     2221 ggcgatgacc gtcaggccgc cgcccgtcac agcacccgcc accacggaat atttgacggc
     2281 ttccggcagg ttgggaaccg tagacgccag gaaagtgacg gcggcattgt cattaaatgc
     2341 cgtcagcagc gtagccccga ccatggcgta gtcccccagc cgcgtcagca ccggctccag
     2401 ccaccaggcc tgcacgccgc ccagaatgac cagccccgcc aggaagaacc ccaccatgat
     2461 gggcacacgc agagacattt ggttctggta ttgcggcgta gccagtgtga aaccgataaa
     2521 gaacaggaaa ctgccgatga acatgaccgg ctcatgggca aaaagaaccg tccagaccag
     2581 gaagcagaca tgcgccaggg tcacccacaa cggtatcttg tcattgcggt cttcccagga
     2641 ctggggaacg gcgtcgtcaa actcgtcgaa ctccctctgc tgggaagcca ggcgggcgaa
     2701 ctccctgcgg aaaaggagaa aatacgccaa attggaaatg acgatggcgg aaacggcctt
     2761 ccatccgaaa tgctggatca tgtgagccag cccccagtcc cacttttccg ccaccatcac
     2821 tacgggcgga gctgcaaagt gcgtcagggt gcccccgatg gagatattca cgaagagaag
     2881 ccccagagtg gcgtacatca aggttttctt cggcttgagg tcgtaaaatt ttttgcttag
     2941 gatcagggcc ccgatcgtca tggcggcagg ttccgtaatc agagaaccca gcaggggagc
     3001 caggcacatc acggaaatcc accacgcgcc cggcgttgcc ttcccaagcc tggctccgca
     3061 gttcacggca tattccgcca gcttgaaaat agggcgggaa gaagcgataa tcatgataat
     3121 catcacgaac atcggctccg tgaaggaaca gtcataattc aaataggcgg aaaaatcctc
     3181 aaaggaataa tacttccagc acacgaacat gaaagggata agccagattc cgaacacggc
     3241 ttccacttct cccagaaaat ggaaaatggc ggacgcgaag gaaaccggga ggcgctcttc
     3301 cggatgcatc acgcggaagt tggtttcctt gagcttttcc ttatagcgca gggccagctt
     3361 atgggaataa cgctcaaagc ggccggccag aaacgtatgg ataatagcgc cgaagaaaac
     3421 gagcgtcact gccaggttga aaccgccgga ctgcttgttg cgttcactaa gcacctggta
     3481 caggctggca tccggcggca ccggattgta atgattgaga ggaatgggaa aatggctgta
     3541 ctgggattcc ccccccgctt ccgcggccgg ataagcggga gccgccgtgc gggccgaagc
     3601 ttcactggcg ggagccccca ggaaaaaagc ggcggcagct acggcagtga gcagacccgc
     3661 acggtgcgcc agaactttga agaaatgaca aatacagatc atgtctgcag acagactaaa
     3721 cgcctctcct tttttccgcg agcggaaaat gagtctgcat gctttttagc gataacgcat
     3781 tcaaacggga aaatccagac atgccccgga aaaaacgcgc gcggcacgga acaggctgtc
     3841 atgcctgcct gagtttaagg ttgaacagaa aacttaaaaa tagtagttgc agatatgcgc
     3901 tccgtcccag gcttcctttc cgttttcttg atcgcagccg gtgtcgcatg tgcggagctt
     3961 ccgcccgacc tgccgccggc gtctcccctt ccctttactc ccggcccggt catcggggga
     4021 accatcaagg gaaacccggc cgccggcatc gttcccgtca aagacctgat tgaagccaac
     4081 cgcaaggatg aatacatccc catagcggaa cccattcccg gagagacagg ctcttccgct
     4141 cccctggcgg agtccatccc taacgcggaa cccattcccg gggaaacgaa cctgcacgcc
     4201 ccggtacaac ctgcggaggc atcccttgcc attccctcca cggctttttc ccccgttccg
     4261 gttcctcagc atgaaacccg cgtagccatt ctgggttacc atgatttcag ccgcaccctt
     4321 cctgctacgg aaatgcgcat gaatacggat gtattccgct cccagatgca ggcgctgaag
     4381 gcgtccggag tgcccgtcat ctccatgaag gaattcctgg aatggaaact gggggacagg
     4441 aagcttccgg ccaaatgcgt catgatcacc atcgacgacg gctggaagag cgtctacacg
     4501 gacgcctatc ccatactgaa agaaacggga ttccccttta ccattttccc atacacaaaa
     4561 ttcatcacgg gccgcggttc tgccatgagc cccgcccaga ttcaggaaat gctgaataac
     4621 ggcgccaccc tgggcagcca ctccgtcagc cacctgtacc caagatcctg gagggccgcc
     4681 cagcggaaag gtacacaagc ggttctggat ctggcaacgg cggaaatagg caattcccgg
     4741 aaaatccttc aggaaaaatt ccccggttct tccgtggagg cctattgcta tcctggcggc
     4801 ttcatcctgc cggaaatgat ctccaaggcg gaagaagccg gattccaggc ggcctttacg
     4861 gtaatcccca aaaaagtgac caaggacacg gaccgctggc ggattcaccg ctacatggtc
     4921 ttcggcaaag accctaagac atttaccaga gccctgaatt tcaacgttcc ctccactccg
     4981 gaaaccccgg cgaccacccc tggcaacagc agcgggaaaa agatgaattc ctacccggcc
     5041 ccggcccagc ccgtttatcc ggccgccaat a
//
LOCUS       DAWEMX010000157         4893 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6453_length_4893_cov_7.860273, whole genome shotgun sequence.
ACCESSION   DAWEMX010000157 DAWEMX010000000
VERSION     DAWEMX010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6453_length_4893_cov_7.860273"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    792..801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1728..1737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1118 a         1500 c         1317 g          938 t
ORIGIN      
        1 ctgcaaaaac ctgcctcatc tggaagacga agtaagggtg atcggctacc ccatcggcgg
       61 caacagactt tccgtcacgc gcggcgtggt ctcccgcatt gatttcacca cgtacgccca
      121 cccccgcaac acggaacacc tgaccatcca ggtggacgcc gccattaacc cgggcaacag
      181 cggaggcccc gtcctgatgg ggaataaagt catcggcgtg gcattccagg gcctgaacaa
      241 cgccaataac acggggtacg tgattccaac ccccgtcatc cgccactttc tggaagacat
      301 caaggacggt gtctatgacg gttacgtgga catgggcatt caggctgcgc ccatcctgaa
      361 cccggccatg cgcaaggcgt tcggcctgcc ggacgacgaa aaaggcgtcc tgatcggcaa
      421 ggtgctcaag gggtcctccg ccgacggcgt gctccgcaac ggggacctgc tgatgaaagt
      481 ggacggctat gatgtggaca gttccgccat gattgaactg gacggccaga aaatcagcat
      541 gaaagaactc attgagcgct gttttaaaga cgaccggctc ccgctggata tcattcgcga
      601 cggcaagccc atgaaggtgg aaatggtgat gaagccatcc ccctccagag accttctgat
      661 ggcggaatac gataaaatgc cccgctacgt cgtctttgga ggcctggtgt tccagcccat
      721 ccagcgcaac gtgctcgcgg cggcggacat ctccatgctg gacgttgcac tggacatccg
      781 gaattaccag gnnnnnnnnn ngctggatga tgaggtaaac gcgcgtctgt ccggctccat
      841 ttccaattcc attgtggaaa aaatcaacgg cgtaaaggtt aaaggcctta cccacgccta
      901 cgaactcctt tacccggaga acatgccgga atacgtcgtc atcgaactga aaaacggaga
      961 gcgccccctc gtctttgaag gcaaggccat ggaaacggcc aacaagcgta tctccaaaac
     1021 ctacaatatc ccgaaaaacg cccgcctgga cagcgccatt cccggccgcc agcctggccg
     1081 caacaccact cccgcccgtt aaccgccatg aaaattcttt ccactttttt cctcggttcc
     1141 ctgatgttgc tcggcggctg ccagccgcgc gaagccggac gaaccgctcc cgacccccag
     1201 cctgaacaac agacggaaac gcctgcggaa caggtggagg aaacgcccgc gccggccccg
     1261 cttccctccc ccaccggctc catggtcggt attaacgcca ccaatcaggg ctatgccatg
     1321 attcagccgt ggagcaagga aaacccggcg tacagccagg gtttcggcat ttatctggga
     1381 gacggcaata ttctgacggc agccaacatc gtttattccg ccagcttcgt ggaagtgacc
     1441 tccgcggacg gctcccagac ggttcccgtg accgtaaccg cctttgaccc ggaagccaat
     1501 cttgcccttc tgcgcctgaa aaacggaaaa gatgccgctt ttctggacaa actggtcccc
     1561 gttgcgctgg ggaaggctcc ccgcctggga gacaaggtaa ccttctggca attcaatggc
     1621 gacggccttc ctatcactac ctccggaacc cttctggcga cggaaagcgc ctgcccgttc
     1681 acgaacgggg aaccgttcgt cctgtataac gtcaaatcct ccgtcacnnn nnnnnnnggg
     1741 gcaatgaact tgtgggcctc agcgccagct gcgatccctc cgcacagaaa gtgctggccg
     1801 taacccatac catgatttcc cggttcctgg aacaggcccg ggccggcaat tacaccggtt
     1861 tcccggcgga cggcacccag gtcacggaac tgaccgaccc cgtcttccgc aaattcttgg
     1921 gcctgcctga aactggcggc ggcttttacg tggtgaaact gcctgtttac ggctccttct
     1981 acaaagccgg agtacgtccc ggagacgtgg tggaaagcgt caacggcatc cctctggaca
     2041 gcaaaggttt aattaaggat cacgccctgg gccccgtttc cgccaacttt ctgttccgag
     2101 actccgccaa gccgggggat accattacgc tgggcatccg ccgcaaggga aaggacggct
     2161 ccagccagcc catgacgctg gacgtcaaac tggacaggag cgcccttgaa ggggacctgg
     2221 tcaatccggc ccccttcatc tccaatccgc cctaccgcat ttacggaggt ctggtatttg
     2281 tcccgctgac gggagccctg atgggagaaa tcaacaagct cagcaagaac catccccccc
     2341 tcaacctggt ggaagccact caaaagaaag aggacatacg gaaaaaaggc gtggatgaaa
     2401 tcgtggtctt cctgatggcc ctgcccaccc aggctacact gggatacgcc cagatgagtc
     2461 cctccattgt ggaaaaagtc aacggtgtgc aggtgaaaag cttcaagcac ctcaaccagc
     2521 ttctggacct tcccgctccc ggcggcacgc accgcatcga agtgacccag cagccgtaca
     2581 ccatgtacat gtcccagaag gaagctgcca aagcggaccg cttcatccag atgagggccg
     2641 ttcccgtgct ccgcagggac taggaatccg cgagccatgt tcacttgcag ccctcctggc
     2701 atgtcacttc acacgctgaa tatccggctt gattttccct acaggacagg ctttacccat
     2761 ggagccttcc gcccggaaaa cgatgctctg gccagcctga tggaacagag gcccggaagc
     2821 cgcgttctcg ttctcctgga aaaagggctg gaacagtttt atccgcagct tccgtcagac
     2881 attgaccgct attttggaaa aaatgccggg gtgctggcct atgccggctg ccgttccgtt
     2941 ccgggcgggg aggaagccaa aacaggtttt tccgcatggg aaacggccct gcgccacatt
     3001 gtggaagcag gcattgaccg ccattcctat atcatagccg tgggcggcgg agcgtttctt
     3061 gacgtagcag gctttgccgc cgccaccgcc catcgcggca tccggctgtt acgcatcccc
     3121 accaccaccc tctcccaggc agactccgga gtaggtgtca aaaacgggat caatttcatg
     3181 ggccggaaaa actatctggg caccttcgcc gtcgcgtggg ccacgcttga cgacttcctg
     3241 ttcctccatt cccagcccct ccccctgaaa agagccgggc tggcggaagt agtgaaagtg
     3301 gcggtggtga aagacgcctc tttcttcaac tggctggaaa cgaacgcctc cgcgctggct
     3361 gtatgcgaac gggatgctct ggaatatgcc gtggagcatt ccgctctgct gcacgcctcg
     3421 cacattgcat gcggaggaga cgcctttgaa ctgggttcca gccgccccct ggacttcgga
     3481 cattgggccg cccattacat ggaaaccatg tccggctaca cactggggca cgcggaggcc
     3541 gtttccgtgg gcatgtgcct ggacatcctt tattccgtcc ggaaaggatg gctgcctgcc
     3601 gcggaggcgg aaagaatcat ctccgtgctg aaaaccctgg gactgcccgt attccacccc
     3661 ctgctttccc gcaggacaga aaacggcgcc tgcgaagtgc tgaaaggtct ggaggccttc
     3721 cgcgaacacc tcggcggcca cctgacggta ctgatgctta ccggaatagg ccggggaaaa
     3781 gacgtccatg agatagacgc cgctctgatg gaggaatgca tccgggaaat gcaggaagcc
     3841 gccggatatt ccattcagga ataacagccc tttccttgcc gcctccccgt cagaaaacaa
     3901 gcagaagcca tctggcgtat cacagagaaa taccagccgg acggcagtat gaaaaaaaca
     3961 acgacgtttt tccttcccac aatctccata tcctgctgtt ccggcagttc ccacagggcg
     4021 gcattattat gtgctgccgg gcagcccatg atgcaagcca ttttgcgact cccccttttt
     4081 tcataacgcg ccggatttac ttctcctttt tcaaaaagaa gatgaaggaa aaagcatgaa
     4141 cagcctgccg gatacctcaa agagaagatc atgccgtcaa caatgcggag gcagtggaca
     4201 cgatgccccg ctggaaacaa atccattcat cgtctttgca aggcacggga aaaccaaact
     4261 gccgggagcc gcgttccgct gaaaattcag ggagggtctt ccggaaaacc gtccctttta
     4321 aacctgcgcg gataactccg cgggaaacgg aactttcccg tattcgtcca aactgacgcc
     4381 gggccatccc catttacaga cgcggtacgg cggcgagaag cgcctgcgtg tattcgtggg
     4441 aaggggaaga aaatactttg tccgcctccc cgtattccac aatgcggcct ttgcgcatca
     4501 cggcaatgcg gtccgcgatg taatggacca cggacaaatc atgggaaatg aatatcatgg
     4561 tcaggctcaa gtcgcgggcc agggcaataa gaaggttcag aatctgggac tggatggaaa
     4621 cgtccagcgc ggaaacgggt tcatccgcga taaccagcct gggttccggc gccaatgcgc
     4681 gcgcaatggc gacgcgctgg cgctggcctc cggaaaattc atggggatac ttgcgcatca
     4741 gggaggggct cagccctacc ttttccatca gttccgccac atcatccctc gtctgggcaa
     4801 aagttctttt acggcggctc tgcaacgcct cctttaatgt tgcatatacc gtaaaacggg
     4861 ggttcaggga agcataagga tcctggaaaa cca
//
LOCUS       DAWEMX010000158         4885 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6462_length_4885_cov_13.440994, whole genome shotgun sequence.
ACCESSION   DAWEMX010000158 DAWEMX010000000
VERSION     DAWEMX010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_4885_cov_13.440994"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1007 a         1689 c         1224 g          965 t
ORIGIN      
        1 cggccgactt ggaaaccgga accgtcaaaa aagcatcctg gaccgttttg ggcatctttg
       61 cggtagcggc cctgctctgc ggagccgccc tctcctggtt cgcctccgcc tatcctgacg
      121 ggctggaatg gtctgtagcc ggagtcaccg ggtcggaaga aaccaggctg gcggaaccgt
      181 cccccctcca ccggaacctg gaaaccgtac aggaggccac cgccattatg ccggactacg
      241 cctttcccgc tgatgacggg gcaaacaccg cagaaacgga agcttcctcc tccattgtca
      301 accctgaaac gagcatggcc ggcgtgctgg gaagcgccat tatcgcagcc cttatttttg
      361 cggccggttt cctgttcggc agaaaaccgc acagccactg cccccgctga ccctccccat
      421 gccactgttt acggacgcct cgcagcaatt ccgccagatg gatcggtttt cctgcggaaa
      481 taccggcatc caccgtctgg acgcccgcat taaaatcgga gctttcctcg tctatcaaat
      541 ctgcgttctt tcctggcctc cgcaggaaat tacggggctc ctgcctttct tcctgttccc
      601 ggtctgtgtc atccgcctgg cgggccttcc cctgggctac cttctgaaac gtaccctgtg
      661 gctgctgccc gtcgccgtca tgataggcct cttcaaccct ctggtggaca gaactcccat
      721 gggagaatgg ttcggcattc ccgtcacgca gggcatgatc tccttcatct ccatcatcct
      781 gaggtgcatg ctcaccatcc tgggtgcctt cacgctgttg gcctccacag gctttgcgcc
      841 tctctgccgc ggcctgaggc aactcggcgc tccgcgcatt ctaatcacgc tgatgacctt
      901 cctgtaccgg tacgccttta tcctggtgga tgaatgccaa catatgctga tggcattccg
      961 ctcccggcgg ggaggctccg ggcccatccc tctttccacg tggggggcta tggccggaca
     1021 acttctgctg cggacattcg ggcgggcgga acgcatttac caggccgtcc tgtgcagagg
     1081 cggggaagat cctgaattcg tcaattcccg cagcgtcatc actggccgtg gcgcagcggg
     1141 aggccttctt ttctgcattc tcgtcattct gttccgctgt ttcaacatca ccatgctcgc
     1201 gggccagtac gcccgcagcc tcatcccatg agccaccacc ttgtagaaac tattgacttg
     1261 tgtttcagtt acccggattc ccctccggcg ctgaacggcg tttccctgca catcacccac
     1321 ggggaatccg tcgccgtcgt aggcggaaac ggcgcaggga aatccacgct gctgctccac
     1381 ctcaacggcc tgctgacccc ctcttccggc caggtacggg taggggacat tcccgtgacc
     1441 cccaaaaccg tcgcccgcgt ccgggaaagc gtgggaatgg tcttccagca ggcggaagac
     1501 cagctattca tgcctactgt ccgggaagac gtggccttcg ggccgctcaa catggggctc
     1561 cccccggagg aaatccgccg ccgcgtggaa caggccctgc gggatgtcca tgcggaagcc
     1621 ctggcagaca aaatgaccca ccatctttcc ggaggggaaa aacgggccgt ctccatcgcc
     1681 acggtcctct ccatgagtcc ggacatcctg gtgctggacg aaccgagcgc caacctagac
     1741 cctgcctccc gccgtaccct catcagcctg ttgcgccaat tcacccatac caaaatcatt
     1801 gctacccatg atctggacat ggtcatggat ctgtgcacgc gctgcatcgt aatgaaagac
     1861 ggttccatcc tggcagacgc tcccgttccc gacatctttg cagaccgctc ccttctggaa
     1921 gaagcgcgcc tggaacaacc cctcagctac ctcctgatgc ggcaggagag gcaacggaac
     1981 aattccgggg catcctgaaa aaacctcata caaactccag ccgggcctcc ggcagggact
     2041 tgagaaaacg cattccatac ctcttggtag ctacgcgcgg atcaagaatc accaccatgc
     2101 cggaatcgct cctggtgcgg atcagccgcc ctaccccctg ccgcagcttc agaatggcta
     2161 caggtacgga atattcgtaa aacggattcc ccccgcgttc cctgatactc tcaaaacggg
     2221 actccacaag cggatgatcc ggcacttcaa acggcaagcg cgtgacaata acgttggaaa
     2281 gagattctcc cggcacatcc accccggtcc agaaactgtc cgttccgaaa agcacgctgt
     2341 ccacatcctt ccggaacgcc tccagcatgg catgccgctg catatcctgc ccctgcacat
     2401 acagcgtcca tccctgttcc tcgcaaaaag gacgcacttt ctccgccgcc tgaaccatca
     2461 gacgatagct ggtaaacagc acgaaagccc tgccgcggga ctccgccaga taccttttaa
     2521 tccattccgg caaaacttcc gcatactccg gctggtccgg ctccggcatg gagcgggcta
     2581 caatcagccg catctgctcc cagtaattaa acgggcttcc gatctgaagc ttccgaacgc
     2641 tttccgcccc tacgcgcccc gcaaaataac tcatatccgc atctccggtc cccagcgtgg
     2701 cggaagtcag aatggcggaa cgtccggcgg aaaacaggcg ttccctcaaa atatctccca
     2761 cctccacggg agcggagcaa acgctcatgt tccttccatc cggaccggaa cgctccgccc
     2821 aatatacgtg accttcctcc gtcatgtcca tcaacacctt cagggaagcc tgcgcctcct
     2881 ccatccgggc ggccatatcc gccagctcat ccttcacggc tgcattttcc tcctgcttct
     2941 ccctctccgt tttcaattcc ccgatcagtt ccatcaaagg ctgagaaaga atatcctgcg
     3001 accactccgg ctgaagaatg cggacaatct ttccggaacc agcaaacccc aggtcatccc
     3061 gcgcattctg gaaaaacagg ccggaggcgt ccatcacgtc ctgaacccgc tggaacaggg
     3121 acggattgtt cagcggtttc agcaaaccct tgtgcgtgcg cggattgtac atccgcagca
     3181 attcataatt caaatgcggt tccgagagct ggacgcctag ctggcgggcg gcgatgttct
     3241 caatcatgtg agcctcgtcc agaatcacaa aatccccggg aaacacgaac cccgcctcct
     3301 cggaatcctc cgcctgggcc atcagcccga aaaacagggt atggttaagc accactactt
     3361 tggcctccag aacccgcttg cgggctgcct ggtacggaca ggaagggccg caatggcgca
     3421 tgctgcacgc atgcggttcg ctgcagacca tggcccatac cttggaagac ggccggaatg
     3481 ccatatcgct cagggtaccg tcctgcgttc tcagggccca gtcctggatc ctcttcaact
     3541 ccgcggcctc accctgcgta aagagggaat ccatctgcgc cagcgcgcgc cgcaggcggg
     3601 tatggcacag gtaattctgc ctgcccttca gcagagcggc ggaaaaatca atccccaggg
     3661 cggtgcgcag cagaggaata tccttgttga acaactgctc ctgcaaattg atggtatgcg
     3721 tggaaatcac cgccttccgg tcaaaatcca aagcaaactt caccgccggc agcaaatacc
     3781 ccagggactt gcccacgcct gtgccggcct ccaccagcag cggcgcatcc acctggagcg
     3841 ccctcgccac agccagggcc atgctctgct gctccgcccg gtactcaaaa tccctggcgc
     3901 gggaaaacaa tccgtccggg gcaaatgccc ggtggacata agcctcaaaa gcgctgacgc
     3961 cttccatgcc ctcttccccg gcggccttca tatgtgggaa cctgcccccc ttactggccc
     4021 acgggaagct ggcgggaaga aaccggcgga agattcacct tttcaggcag gtcatagaac
     4081 atggagcgca agccgttcgc attcattctg gaagcgtcga tgaacgtcca gggcccctta
     4141 tccttcaaat ccttcaccag caccagaaca ccggtattct ccaccttcac gggcaccccg
     4201 tccggacgct tcatggaaat gaccatccgc gtgggaagca ccaccagcac ctccccgccg
     4261 cccttcacca gatgttccgc cgcaggctgg cctatttcat acgtctccac caccatgccg
     4321 cgttccttca tcacggcggc ggcggcctgc atcttttccc tcatggtctc catgaaggag
     4381 gcctccccgc ctggaaagga agcaacaagc tgcttcttca tgggcgggta catcttctca
     4441 atcatccaca tcatgtcgcc cgtcttcacc gccccggaaa gctcccgggc ggcccggacg
     4501 gcgctgtccc catattcacc ggcctggacg gcgcatacag ccgccaccat catgcctgcc
     4561 aacaaactct taatcatgga cggagtgtaa cggcatgcat ccccctccgt caaggcaagg
     4621 cctcctgcgg aatcatgttt ttaccgcgcg ttatcctgca tcaccaggcc ggaccacatc
     4681 cataaagtgg ggtccagctc ccgggcggca tcctccatca gcgcgcgtcc ggttccttca
     4741 tcctccacca cggcgaacat ggtggaaccg gaaccggaca tcatggcgcc gcgcaccccg
     4801 ggccgcccca tcagccagcg cttcacttcc gccaggaata aatgcttttc aaacacgggc
     4861 ctttccagat cattgaccag cacat
//
LOCUS       DAWEMX010000159         4842 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6533_length_4842_cov_9.709212, whole genome shotgun sequence.
ACCESSION   DAWEMX010000159 DAWEMX010000000
VERSION     DAWEMX010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6533_length_4842_cov_9.709212"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    784..793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          882 a         1285 c         1552 g         1113 t
ORIGIN      
        1 tcctgtaccg ggttcgtgat gctgcatctg ctgggggatc cctccccttt gtacagttcc
       61 ccgtttgaaa gctttactgt cttttcccag tctctctggt gcatcgcctt gggggcggcg
      121 gcggccgtgg cggccagggg atgggtcagt ttgctggatg cggccgccag atatttgaat
      181 ggccgccagt cccttctgcc gctgcgtttg gcggccgccg ggctggtcgt ggggattctg
      241 gccgtttttt atccggaagt ggtgggcaac ggtcaggctc tcattaccgg gctggtccat
      301 gaggattacg gcacgcggga ggcactcgtc ctgcttctgg tgaaggtgtc tgccgtggcg
      361 gtggtgttcg gctgcggtac ggtgggcgga gccatgacgc ccaccctgta cgtggggagc
      421 atggtgggtt ttatcttcag tacggttctg acgtccctgg gaatggaggg caaccatacg
      481 gtggcgtatg cgatggtggg gatggcgtcc tttttcgccg tagctgccca ggcccccctg
      541 acggccctgg tgatggtggt ggagttttcc atgagcggac agatgattta tcccttgctg
      601 atcggtgtcg taagcgctta tggcacggga aaactgattc gcgcgcgttc catgtatgcc
      661 gccagcctgg ccggcagtcc ggcgtccgtc gtcaacctgc ccatggcgca agtccatgtg
      721 catgacctgg cgcggcacgt ggtgccccag gtggccccgg atgcttccct ggatgatgtc
      781 tccnnnnnnn nnnatgtctc ctccctgatg ctgcggaatc ccggagatct ggtttttgtg
      841 gtgaataagg atggaattta tgaaggggcc atctatccgg aggattttct ggcggtccgc
      901 cggcagatgg aggaacggaa gggggcggtt ccggccgatg ccgggtccct ggtccgtacg
      961 ggggctcccg tactggacgc cagcacccat ctgacggatg ccttgaagct gtttgaacaa
     1021 acccatctgc ccgcctttcc cgtggtctgg aagaataccg gtcggctgga cggcgtgctg
     1081 taccgcaacg cgttgttcca ggttatcacg gagatgatga aacgggagtc cggcggagac
     1141 gtgggcatgt aaaacggaac gaaacaaggg atgtatatta catttttgta aaaatctttt
     1201 gttccccgtt cgtaatatca tcaacattaa ttgctgggat gttatccata ttccccgtgc
     1261 cgtttttttt gcccggctgc atggcggtgg aagaaaaagt agttggtatg gcgggggcgt
     1321 gcggttaata ttcgcccagc cccggattgc atgaatgaaa taaacaggtc accggatttg
     1381 gagatggtcg tgctgaaaga aatcagctcc gccattgtca atgaacgcaa cggcacggct
     1441 ttgctccggc atattctgga cgtgctttac cgccgcatga aaatgctgcg cggcacgttc
     1501 accatccgcc gcgggaacga tttgatgatt gaagcctccc acggcctgga cgagcaggaa
     1561 atgaaacgcg gccattacca tgtgggggaa ggcattacgg ggcatgtggc ggaaacgggc
     1621 aggccccatg tgattgagga tatttcccgg gattccaggt tcctgaaccg tacccgcacg
     1681 cgcaaaagcg gtgaaaaagt agccttcatc tgcgttccca tcattcattt gcagcaggtc
     1741 atcggcacgt tgagcattga ccgtgccgtg gaccgggaca ccgacctgga acgggatcag
     1801 aaactgctgg agattgtagg caatattgtt ggggatgctc tggctgccag cctccaggcg
     1861 catgaggaac gtgccgccct ggtcgcggaa aatgaaaaac tgcgggagct tttgacgact
     1921 aatccgggcg aattgatcgg caattgcagc actatgctcc aggtgtatga acagatccgc
     1981 caggtggcgc ccagcgatgc cacggtgctg atacggggaa gttccgggac ggggaaggaa
     2041 ctggtggcgc gcgccgtggt gaatctgagc ggcagaaagg ataagccgct ggtcacgctg
     2101 aattgtgcgg ctatgccgga gaacctgctg gaaagcgagc tgttcggtca tgaaaagggt
     2161 tcttttaccg gggccaccag ccgccgcatc ggccgggcgg aggcggcgga cggcggcacc
     2221 ctgtttctgg atgaaatcgg ggatttaagc ctccagatgc aggtgaagct gctgcgcttt
     2281 cttcaggaaa agaccttttc ccgcgtggga agcaacaggg agcttcatgc agacgtgcgc
     2341 ttcatcgccg ccaccagccg caatctggag gaactgatgg cggcgagcaa gttccgggaa
     2401 gacttgtact accggttgaa cattttcccc atcgttatgc cggacctctc caaacgcaag
     2461 ggggatgtca tgctgctggc ggaacatttt ctttccaagt tcaacctgaa atacggaaag
     2521 gatatcaaac ggctgtccac ccccgccatc aacatgctca tggcttacca ctggcccggc
     2581 aacgtccggg agctggaaaa ctgcatggaa cgtgccgtca ttacggcgca ggacgactgc
     2641 atttacggtt acaaccttcc cgcctccctc cagatgccca gccatgacgt cccttattcc
     2701 cgtgacgggg aggctcccgc cgatttgccg accatggtag attcctttga acgggaattg
     2761 attgtagccg cattgaaacg ttctccgggc aatatgtccg ccgcggcccg ggaactgggc
     2821 atttccccgc gggtactcca ttataagatg cacaggctgg gcctgcagaa atcatgaaag
     2881 gccttgacca tcttttatcg gccgtctccc ggtcctttta tctgagcatg cgcttcctgc
     2941 cccgggaaat gcgccccggc gtggccgtgg gctatctgct ggcgcgtgcc acggatacgg
     3001 tggcggatgc cgtggacatg gattgccggg agcgcctggc gcttttgagg atgatggggg
     3061 agacggtggc cggctctgcc tcccgggagg ttgccgccac ctgttttggc cgtctgggcg
     3121 ttctttccac aatccagacg caccgggggg aggcggaatt gctggcccgt ttccccgaat
     3181 gcctggcgct gctggaaaac ctttccccgc atgaacggat gctggtgcgg caggtgctgg
     3241 cctccatcgt ggagggacag gcgtgggatt tgatgtattt tgaaagccat gctgccgtct
     3301 catgcccgga acagctggaa cattatactt acatggtggc cggatgcgta ggggaattct
     3361 ggaccaggct gggcttgctg gcccaggggc cgggcttcag cacgcagccg gaggagcagc
     3421 ttttgcgctg gggcaggcac tatggcatgg ggctgcagtt ggtgaatatc ctgcgtgacc
     3481 gggaggaaga cctcgcccgc ggaagaagct atttgcctgc cccagatgac cgtccatggc
     3541 tggaccgcgc ggaacgctgg ctgaaggaag gcgtgtttta cgcggaatct ctgcggaacg
     3601 ggcggctgcg tttttccacc gtcctgcccg cctggctggg gctggacacg ctggaactgc
     3661 tccagcgggc ggatcggcgg catccgtctt ccgggaaggt gaagattgcg cgcggagacg
     3721 tttatttacg gatgtggaaa gcctttcttt tttcttggaa agaggctctg tagcttatgc
     3781 ggcgcttttt gcggctgatg aagccggagc agtttgcggg gaagccggcg gagccggggc
     3841 aggggcaggc ttgtttccgg gagcttcctc ctttttccgc ttatctgtag gtgtttccgc
     3901 tgtgccgccg ctttccggtt ttggggcggg cggtggcttg ggcgccagca ggtcttccca
     3961 ctggtggagg ttgaatcctt tccccacgcc gcctatttcg ttgagaagtt cccggcatct
     4021 gtccgtaatt ttttcatagt cgagaggccc ggcctcgggc tctgcggaac cggggaaaat
     4081 caggtaactt accttcaggt cggagacggg gcggctgtaa gggcccgcct tggggttgat
     4141 ttcacggctc aggcgcaggg aaccctcccc ggttttgtag cgcggcccgg cgtctcccac
     4201 cagggccggg tacaacttgt ctcccacgat gacgacggca tagtccccga actgcgggga
     4261 gaagggagtg gactggttga cgactcccag cggaaccacg atgaacggat cgtattctga
     4321 gatcaggaag ctgtaccgct tcagttcctc aatgacccgt tccgcatggt ctattttgcg
     4381 gcgcagagcc gttttttccg cggcgggggc tttttccagc gtcttcttgt aggaagccag
     4441 cctctgctgc cagggtttga gcaggggatt gggcttgtct gtgcgctttt tccagcggta
     4501 ggaagtgctg ggctgataga aggagctttt caggatttta tccggcatgg cggccagccg
     4561 gtccccgtcc gagccgtcgg aaacaacatc catttcactt tgcagccaga gaagcttcct
     4621 tcccgtgtcc ggataggtgg tttccagaat cgtctccgta tcgtaataat tatggcggtc
     4681 cagaagtttg agcagggagg ccaggttttt tcggatttcc gtctgtttgc ggacgtaaat
     4741 ctgcccgtag taaggggaga cgcgcgcatg cctgaacagg gtggacaggc cgggaagcat
     4801 gggttccagt ttcggattga gcttcagcat atcttccttt tt
//
LOCUS       DAWEMX010000160         4780 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6618_length_4780_cov_7.687619, whole genome shotgun sequence.
ACCESSION   DAWEMX010000160 DAWEMX010000000
VERSION     DAWEMX010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_4780_cov_7.687619"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1093 a         1405 c         1206 g         1076 t
ORIGIN      
        1 agtacaaaca tacgccaacc ccggtggaat atagcatttt gctggttctg cagccacccc
       61 caattgaagg tgatgtcccc gcctttacac aggctctggg ggattttccg gatttttaca
      121 tgaagaccaa ttccctgatt tcctattatg accagtcttt tgtacgccgc aggctttccc
      181 ttcacaagaa ggcggaaaca gcgattgtcc ttctggacgg caacggcaat atcatctgga
      241 aggatgacgg cggtttcacg gcctcacgcg cggagcattt gaaagttatc ctggaaactc
      301 tttctccgct ggcacccagg acaagaacgc attaaaaggg gaatgcgtct tcttcgcctc
      361 atccgcactt tccgtttgag gttccctccg gaatatccca tactggcctc atgcctacca
      421 tttctcttgc tctcggtgaa cgttcctacg acatccacgt ggaaaacgga gcactggacc
      481 gtaccggaga gtttgcctac cgcgccggtc tggacggcaa gattgcggtt atcacggaca
      541 gctgcgtcgc cccactttat gcagagcgcg taatgaagtc cctggagagc gcgggattca
      601 tgcccagcct gcacgtagtg aatgccgggg aagcttccaa aaatatgctt caggcccagg
      661 agctgtgttc cgaactggtc cgggccgggc ttgaccgcac cggatgcatc gccgctctgg
      721 gcggcggcgt agtaggggat ctggccggct tcatcgcttc catttattac cggggcatcc
      781 ccttcatgca aattccaaca acggtggtcg cccaggtgga cagttccgta ggaggaaaga
      841 ctgccgtcaa tattccagaa gggaaaaatc ttgtaggagc ctttcatcag ccgtccgtcg
      901 tggtaatcga ccctaccacg ctggtgactt tggatcgacg tacgctggcg gaaggtttgg
      961 cggaagccgt caagcacgca gctatcaaag acgcttccat gcttcatgaa ttgaaagggc
     1021 tggggccgga actctccatc ggcttctccc tgaatactat tgccaggctt cctgacctaa
     1081 tctcccggaa cgtggccatt aaggcccgca ttgtagaaaa tgatgaattg gaaactctgg
     1141 atatccgggc tctgctcaat ttcggccaca ccatcggcca cggcattgaa gctgccgtcc
     1201 cttacggaac catccttcac ggggaagcag tggcgctcgg catgcgcgcc gccctgtttc
     1261 tgagtggacg gaaagccggt ttaagttctt ccgatgccaa caagatcctt cgcacgcttc
     1321 aggcgctgga acttcccttg gttcttccgg acggcattga tacggcaact gttcttcaaa
     1381 aaacggcatc agacaaaaag ttccgggccg gaaccatccg ctttgttctg ctttccaaag
     1441 caggggaggc aggcatttct acgaaaatta ctcgggaaga tatggcggaa gccattgaag
     1501 aattgcgccg ccccctggat tgaatttacc acccgtggcc ctttgttctc tagcaagaaa
     1561 actccgggag tcgggctgta cagatatttt ccccttctct tcgcgcattt tcccatcagg
     1621 aagaggaaag gatttaccgg gggacctgat accatatcaa accgcccttc attccggcaa
     1681 aacggaatga agaggaacat tgtagtaatt acccaattcc cgacacgggg aatttatttt
     1741 tacggctctt ccgcggtagg ttcaaatcca tccacatcca gaggcacact gcccgtgtat
     1801 tccccggcat cgaaatgaat tcctttcaaa gcatcttcca gagacgaaga accataacac
     1861 tcccggctgg ccagacgcat caattcattt gcaatattta cggcttcttc attgggaata
     1921 tccccgggca gggaggcagc cagctctttc acctccgccg cgtgggcgtt tgccgtagcc
     1981 tgatcctgaa taagcagcag aacggccaga attttttctc ccggagtttt aggaacggcc
     2041 tgttcgctgg gaaccgaaac atcccgggga tgatcctgct tcttgccgcc gtcacaggca
     2101 acaaacacta acgtcgccaa aaacgccgta aaaggaatga gatttcgctt catcatgatg
     2161 gttggttgaa aagccggagg cctcattgaa ttcatcatga acactgagga ttccggagtc
     2221 aaatcagttt ttatccttaa aaactgtttt tcaggcaaca tatctcttta acctctgcct
     2281 ctcgttctga acgcaggaaa cagcccttcc catttaatag gcaaaatata gggagttcat
     2341 tgactccctc cgcagagcac catttccgag aatgacggtt gcgcctattt tttgtgtcac
     2401 catttcaata cgacgtttga tttcacagga cgtcaccgta taattcccct gagatgggtt
     2461 accctatttg gaacagacgc cgttttttga agaccgccgc aggaacagcc ggattccttg
     2521 ccgtcatgcc ctgcctgaac gcagaagagg ccctgacgaa cgcaaaaccg ctcaaggtgg
     2581 gcctggtagg ctgcggaggc cgaggcctgg gcgccatgaa aaacgctctg gacgccgatc
     2641 ccggaacaat tgcctgggca ttggcagacg tgtttcagga acgtatcgat ttcggcgcca
     2701 aattgctggc ggaacagtac ggcaaccgag cccggctgga caggagccgc ctattcagcg
     2761 gcctggacag ttacaagaga ctgctgcaaa cggatattga cgtagttctg ctctgcactc
     2821 cccccgcttt ccgtccggaa catttggcgg cggccgttga cgccagcaaa catatttatg
     2881 cggaaaaacc cgtggcggtg gacgttcccg gcgtgctttc cgtactggaa tccgcacggc
     2941 tggcaaaact caaaaatctg gtgattctgg acggcttctg ctggcgctac gacaaggcga
     3001 atgaggaagc ccaccgcaag ctttcctccg ggcagctggg ccgtgtcctc tcctttgacg
     3061 ggttatatta taccactcct cccaagtctc cgctggccct ggattcccga ccttcctccg
     3121 atacggacgt ggcatgggcg ctacgcaact ggaccgcatg gaactggctg agcggaggcc
     3181 agtttgtgga acaaatcgtc catacgattg acggcatggt ctggtccatg aacgagcagc
     3241 ttcctctggc ggctttcggc tccggaggac gcgcccagcg ccgggatgac ggcgacgtat
     3301 gggatcatta cgacgtttat tttgagtacg gccacaacgt aacggcccac atctcatgca
     3361 gacagtgggt tggctgccac ggggaaatcg tggaccgcac catctgcgaa aaaggaatgc
     3421 tggttactcc ctaccgcccc tacattcagg cgaataaccg ctggcgtttc cgcggggaac
     3481 gcggcaatat gtacgcagac acgcatgtgg ccttttaccg tttcctgcgc tccggcacct
     3541 ggacccagac actggaaagc gcagccaata aaaccctggc tgccatcatg gggcgtgaag
     3601 cggcccacac aggtcagcgc attacctggg aacagattaa gaaaagcacc gcacggctgg
     3661 tgcctgaaga cctgacgatg gatacgcccc tgccgcccgc ccgcatcccg cgcccgggca
     3721 gaacggcctg acggaggcta catccctttc tctccacggt tcatggccaa catctttcca
     3781 cccaatacca acaagcgctt ttacgggatt gccgccctgg ttgcgctcgc tgccgcgttc
     3841 gtactggggg gaatatatta tgttaatttc agcattccgg agtatgaacc cgtgcagccc
     3901 gtccgctttt cccacaaact tcacgccgga gatctgaaga tgaactgcac ggcttgccac
     3961 agcgccgcac agcgctcatc cagagccggc attccggata cgaagtcctg tctgggctgc
     4021 caccagcata ttcttccgga cagcccgctc atcgcccccc tgagggaggc ggcggatccg
     4081 caataccccg gttacacagg agaaccggtc cgctgggtga tggtcaaccg cctgtccggt
     4141 cacgcctatt ttaaccacat ggcccacctg aaccggggca tcggctgcac gtcctgccat
     4201 ggagacgtgg cgggcatgga acgcatcagg gctccacgcg atgcccgcat gcaatggtgt
     4261 ctggactgcc accgcaatcc ggctccccat ctgcgcccgc tggaggaaac ggccagttcc
     4321 cgttattccg ccgcagacta cctccgcacg cattccatcc gggatgaaga agggaaaagc
     4381 atccaaaccc cgcttcaact gggaaatttc ctgaaacgcc aatggaaaat tcagccgaaa
     4441 acggattgca ccgcatgcca tcactgacct ccatggcctg tttgacatcc gcggcgccaa
     4501 agttattcct ccctgcgttc ccggatactg cgtaataaaa agaccaaagg gcttttcatt
     4561 aaatgattcc ttccttgaat ccgtaatcaa tgacattgaa ttccacaaga aagatttcct
     4621 cctgttccct gtttatccct tattggcccg aagataaaga ctaccgtcaa tcaccacttg
     4681 atttcatcct tcggatttcc taaaaaaagc catccctgtt tcatcaatcc ccatcattac
     4741 ccggtttgac tcccctccct ccattttccc agaatgaagt
//
LOCUS       DAWEMX010000161         4731 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6693_length_4731_cov_8.794910, whole genome shotgun sequence.
ACCESSION   DAWEMX010000161 DAWEMX010000000
VERSION     DAWEMX010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_4731_cov_8.794910"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2270..2279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2717..2726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1202 a         1299 c         1126 g         1084 t
ORIGIN      
        1 aagtaccccc gaccggactc gaaccggtac gtccttgcgg acaacagatt ttaagtctgc
       61 agcgtctacc attccgccac gagggcattg accggacggc actctacagg aatgccgccg
      121 ggattcaagc acgaatccct tccggttttt aaatattaat cacgaacttc caccaccatg
      181 ctgatttcca ccgctacatt gagcggcagg gcggatacgc ctaccgccgt acggctgtgg
      241 cggccaattt ctccaaaggc ttccaccagc agatcggatg cgccattcaa tactttagga
      301 tggtcataaa aatccgggcc ggcatttaca aatccgttca cggccacgat tcgggatact
      361 ttttccagag accccaccgc ctgcacaata acggccagac gattcaaaat agcggcgcgc
      421 gcagcggcct gaccttcttc caccgtcagc tcctgcccta ctttgccaaa gtatttgtcg
      481 tccccgttca cggaaatgcc gccggagagg tgaagcaggt tgcccgtgcg tacgcattgc
      541 acataggagc ctacgggagc gggagcatcg taaatggcgt aaccaaggtc ggaaagtcgt
      601 tgacgtattt tatccatata gagcatttga cagaagttga gttgaaagga tacaccggaa
      661 atagttgcat ttccagatgc ctttcatgac attttgaaca attttcggtc tgtttgcacg
      721 taaaatgatt gccatccacc tcatttagtt gctattgaaa caacccgtac atgatgaccg
      781 acgaaactcc ttccacagac cccaagcagc agattccgga cctttccggc ttggccgact
      841 ttcaatttgg tccggcctgg gcgagaccgg gagcccgtca ggaacggcct gtatcctacc
      901 cggaacgcag gtccggagac aggcagggag gcgaccgccg accgcggcgc gacaaccgtg
      961 aaggccgtca gccccggggg gaacgtcgtg ttgacggtca aagcggcgaa agaaggttta
     1021 accgggaaga ccgcacgcgc ggctccttcc gcaaccgccg cgatcaaact ccccgctccg
     1081 ccatggagga accgacggaa ggcctgcgcg tggagctgcg ccccgtggat tccggcctgg
     1141 ctgccctgca ccaggaagta cagaaacacc gccgcaccat ttccctgctg gatcttgcca
     1201 aggtggtcat gggttcctac gaccgttatg acctggtatt catgaagcag gaaaacggcc
     1261 cggatctgta ccattgcaag catggcgacg gcgcctgctt tatttcccga caggaggccg
     1321 tcaaacacct ctggcagtcc acctggatgc ccaaatatta cgaatccgta gaacaggaag
     1381 tggaagctcc aaaaggagat ttcaaagcca ttgccaaatg ttccttaaac aacgagctga
     1441 taggtcccgt taattggcac ggctaccagt ccgccctgat gaacctgcat cgcaccaaat
     1501 ttgccaacat gacttttgag gctttccgtt ccaagattgt gacggacaag agcgaagaag
     1561 ctgtccaggc atggcaggaa gccgtttcca aacgcaccgc ctggaagccc gttcgggaag
     1621 gagcctccga agtgcttctg gaatctcccg ccgcggtaga acaggatttc gaatccaacc
     1681 actttgacga gtgctatgac gttacggaca aggtgttcgt caacggagcc gtgaaaaaga
     1741 atcacctctc ccccggtctg tgggcgcacc tgatccaatt atccggcacc acccgccgcc
     1801 atcccagcat gctgattccc aacctttgcc acgggttagc ccggcatcat atgcctattt
     1861 tcaagtggaa aggcaaccat tacaccggcc cggcacgccc caaggccatg gaagaaggca
     1921 ccgtcttatc agattccctg atgtccattg ccacctgggc ggccaataat cccggcaaag
     1981 gcgtcgatgc catgcttaaa gagctggccc cagtcccgga acaggaaaag gccacagaag
     2041 aagtagtagc ccaggccatg gaaaaacagc agaatttggt tcgtgatctg ctctggctgt
     2101 ccgaacaggg ctatatcctg gttttctcca ataacactgt cagccgtcca agaaccgtct
     2161 ccgcccaagc tccttctccc aagaaggctc ccaaggaaaa acggaaaccg gaagagaagg
     2221 gccagaacgc gccggcaacg gactccgctg caccagaaaa acagaaggcn nnnnnnnnnc
     2281 ttccgaggcg gcgcccgccc gggaggaagc atctgaagaa aacagcgtgg ttacagaaac
     2341 tgaggaattc ccctccgttc cggcggaaga actccctgaa gataatattc cggaggaggt
     2401 ttccgcaaga gcctgacgcc ttcccttacc cccccttccc gtcatacagt ccgcaggatt
     2461 atcttgatcc tgcggacttt gttttgtaga agtggggaaa atcaacagga ttttacaata
     2521 tgatcagtac aacaatggca actgctctta atgagcaaat caaatgggaa atgtattcag
     2581 ccaaccttta cctggccatg tccgcctatc tgcaggatgc cggtctgaca ggtttttccc
     2641 actggatgcg cattcaatat caggaagaaa ccgctcatgc gctgaaattt tatgatttcc
     2701 ttctttcccg cggaggnnnn nnnnnngaag aaaccgtcaa ggacatcatc agcaaattac
     2761 gcatgattaa gggagaagga cagggaatgc tggttctgga caaggatatg tcagcccgca
     2821 cctttacgcc gcctcccgcc aactaattgt tgctattgtt ctccgtccat tctccggaaa
     2881 atagttccag aaaaaactta ccggagaatg gcgggggaat aaaatatctt gcggatacaa
     2941 gcataaggtt ggcggcatgc aatgatgttg ccgacagtaa tgttttattc catgattttc
     3001 tgcctgcaat gctgcaagca gctggagttg ctgtatggcg gcagctggga cggcaagcaa
     3061 cgccgtcttt cttatttttc aggaaacagg ccgacggatc acaaaatttt ccgttgtgcg
     3121 gaatagcgag gaatgacata agaaccactc gtaaacaata aatgctgaaa aaattaatga
     3181 accacgcatc cccagcaaac cgatatcatt gaaatgtaat ggtaacggct ctaagttcca
     3241 tggtcgtgaa catttgataa cggaccttca gaatatgaac atggacggcg caattgctct
     3301 tttacacacc gtgccgataa ccaatgatgc gtcatctgta atggagcatt tattaatgcg
     3361 gttgatgcgc ccggcccttt cactgccgct ggactatttt caaaataggc cgttccggat
     3421 aataggagtt ttgcaggtaa cctcttagaa ccccggcagt ctttttccgg ctccttgagc
     3481 attttcccct attacttccg ccagcctttg acttttgggg aagaagcaat ttttctctcc
     3541 caaaagcaga gcgtcattcc caaaccgggt ctgtaacggc gcttgctcca tcagtatggg
     3601 gcaggtttcc gtttgttctt ttcccctcct tccaggaccg tttaaaaagg tttttcctgc
     3661 ctttacaagc gtcatagact cataccgccg taaacaagga accgcaacta tcgcggcagg
     3721 aaggaggcca tgaaagaaag ttttcaacgc cccctttctc tactctcacg tcaagactgc
     3781 agaagttcca agtccgtctc aagggcagga aaatattgct tcccgctgta taaaattttc
     3841 ctccggcaag gctttatttc ccatgaaaag cctctgcaca agagccctct tcaatccagg
     3901 cttgtcatgc tgcaacgcct ctttatgcca gatttgctgc ggattcccgg aagttttgtg
     3961 aaaaactacc gtttatcaat agcttatatc tatttttaac atattattca cattctatat
     4021 tcagaatata tccagtctat taacagtcaa catattgtat ttcaaatacc atcaaaatgc
     4081 ttattatccg caaatgtaaa attttaataa aaatttatat ttttgacttg cccgcagaag
     4141 gacgcggcgg taggttggcc gaaccgccgg cgagtctccg gacgggtatc gctatcaacc
     4201 ctcccataca ttcacaacga tcatgagctc catttccgaa gaagcaaaca agctggcgga
     4261 tttcatcctg ttcacgcaac gttcctgcat tctgaacctt tcttcagaat tgaatgaggg
     4321 caaggtttcc tacccccagt ttttcctact gacttatctg gccagcgaag attttctgag
     4381 catgtccagc attgcccaga aaatggggca ttctactgcg gccgctaccg gcatggtgga
     4441 taaattacag gaaatgggct acctgaaacg catgagcgca gccaaagacc gccgcaaaat
     4501 catggtcgcc atcacccagg aagggcgcga tctggtagct cgcatgcgcc agaacatcgt
     4561 tcgggatctg gcagccttga tggccggagc ggatccggac gcacgccaga cgcttgccga
     4621 taccggaaaa tccatcagaa agcgccgttt ggcctgattt ccgatttttc ctcccggtct
     4681 ctcccggaga aaccgttccc ctgttatggg gaaggccttt ttcttttctt t
//
LOCUS       DAWEMX010000162         4590 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_6908_length_4590_cov_8.031753, whole genome shotgun sequence.
ACCESSION   DAWEMX010000162 DAWEMX010000000
VERSION     DAWEMX010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6908_length_4590_cov_8.031753"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    890..899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1651..1660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1008 a         1379 c         1139 g         1044 t
ORIGIN      
        1 gcaactttag cacccaatat tcagatctgg aaatacgacc ggaaaaactg acatattttt
       61 tcaaaaataa atttctattc gcattcccca tttttataga agcatttccc tctatctctc
      121 tgcggatttt atgcatatcg gaagcagata cggaagactt caaaatctcc tgtacgtcag
      181 acgcattaca ctctattccc aactcattat tcacgatgtc tgcaactgct ttagaactcg
      241 tttttgtcaa tattgcgacg tccacaagtt tattgtatgc atcaatacgg gatagccttt
      301 cttccgactg ttccataact tttttcattg gtgatctttt tataaataca gtaactatgc
      361 ctcttctctc ttccctttac attactccca gcttcttcag catggagatg gcaacggtat
      421 ttccctgttc cgcagccaga cgataccaac gtattgcttc ttgcctgttt ttagcgactc
      481 catgcccaaa atagaaacat gcacccaatc ttctttgtgc ttctgcattt ccttttttgg
      541 ctgacaaaaa ataccagttt acggcttctc ttttattttt cccaactccg tttccaaaaa
      601 aaataacagt ctcccagcat cctctgggct tcccgtttcc aaggttcgag ctaaaacatg
      661 ttcacccatc agaaagactg ttccaaatat gattaatgtg gaaatagaga tattcatttt
      721 tacctgttct aacggattcc caatccgtac acttatattg aataatgtag aaaaaaatct
      781 aacatatttt caaaaatcat tgtttatcca cttccatctc ctctctcgtt cttgtttgat
      841 ttttatagct gttatcagca cattttagac ttgtgtacgg attgggaatn nnnnnnnnnt
      901 tgggaatcct ctgttacaac gttcttcatg aacataccta cttcctcttt cccaactgac
      961 gcagaccagg ccggaatctt cctcatccgt tccctgggca ttccgcccat ggacgctttc
     1021 cttcttttaa aggatctcct ggacaccagc cgcggaagag gcgacagaat cacccgggcc
     1081 aaacgctgca tacggctggg aggagaggct cttgccgaca gggaaaaaag cgtatcgttt
     1141 tcccaggctg tccgcgccag cctggaagca aggaaacacc gccgtccccg cacgctgcag
     1201 gaaatccgct atatggccgc ccggatgatg aaaaaatgcc cggagctggc aaggaaacag
     1261 gtccgttcta tcactccgga agattgcggg cgttatctcc gtaaaagctt tcccactccc
     1321 cgccagcggc acaaggggcg gctgatcctg agcggcatcc tgaattattc cctgaagcgc
     1381 ggatggtgcc gcagaaacgc ggcctttctg gttcctcccc ccatcctcag ggaaaaacgc
     1441 atcagggccc tttccctgta cgaggcaaag cggcttctcc acactgcgga acagttgttc
     1501 cgaggggaat gcctgccggc ctgcgccctg atgctgtacg cgggtatacg cccccacgag
     1561 gtcaaaaggc tgacgtggaa gcatatcaat ctgaaatccg gcctggtttc actggcgccc
     1621 acccatacca aaacgggagg gagccgccat nnnnnnnnnn tcccgtgctg ggttccatcc
     1681 tcagccggat gtcttccgcc ggttcccccg cccgttccgt ctgcccgccc aactgggaaa
     1741 agaaatggaa ggaagtaagg cgccggtccg gcatcctgaa gaaaagcgga tgggttcagg
     1801 acgtgctgag gcatacctac gcctcctacc acctggccca tttctgcaat caaaaccttc
     1861 tccagaagga gatgggacac tcctccccct ccctgctgct ggcccgctat cttaatatgg
     1921 agggtatcac ctccgcaacc ggcgccatgt tctggacgca cagctttgtt tctcccgctc
     1981 cgttaaagga agactgacaa aaatccgtct ggaattgcgc ggccaaaggc ggacaatccc
     2041 ctgcgccggg agcgtccgtc gcccctcttt tttcaacctt tccgccattc tccaaccatg
     2101 ccagcaacca gccctccccg cgcctgggcc gaaatcgacc ttggggccat ccgccataac
     2161 ctgaatgtcg tcaaacaggc ggccaaaggg gaatattaca tgcccgtcgt gaaggccggc
     2221 gcctatggcc acggcctgga acaggtttgc cgcacgctgg attcggaagg catcgctttc
     2281 ttcggggtgg ccaatgtggg agaagcccgg cgcatcagcc aggcgggctg ccgcacccgc
     2341 ccttacatcc tgggccccac gttcccggag gaacgggagg aaatcgtgct gaacggctgg
     2401 cgctccttca tttccaccat ggaggaagcc gcgcattaca attccctagc gcggctgtac
     2461 ggaaagacgc tgcccatcca cctttccgtg gatacgggaa tgggccgcgg cggattcctg
     2521 ccggaccagc ttgaagaact gctttcccgc ctgggagaac tggatagcct gcatctggaa
     2581 ggcctgggcg ctcaccttcc ctgcgcggac gaggaccggg agattacgct caggcaaatc
     2641 tcccgctttg aacagatggc cgcccgcatc cgggaaaaac tgcctttgaa gtactgccac
     2701 ctggccaaca gcgccgcctc cctggattat gaaattccct ccaacaacat gtgccgcccc
     2761 ggcctggtgc tgtacggttt ttcccccatt ccctccccat gggccgcgca attgaagccg
     2821 gccatggccc ttttttcccg cctgacggtg gtgcgcacgc tgccgaaagg ccacggcatt
     2881 tcctacgggg gcacctttgt gacggaccac cccaccaggg tggcgaccgt aggcataggc
     2941 tatgcggacg gctacctgcg ttccctggcc cacaagggcg cccgcgtcat ggtggacggc
     3001 gtttcctgcc cccttctcgg gcgcgtgacg atggaccaga tcatggtgga cgtcagccgg
     3061 gcgccgcacg cggaaccggg catgaccgca gaaatcatgg ggccgcatat ccccgtgacg
     3121 gaactggcgg aaaaggccgg caccatttcc tgggagattt ttaccggaat aggcccccgg
     3181 gtaccccgcc actacaccca ctaaaccagg caggagggaa aagggccaag ccggccgccc
     3241 atttctgggg gcttctttcc cgattgacgg acagacagaa atatcgttta gttagccagt
     3301 atgatgaagg tttccaccag aggacgttac gcgctgcgcc tgatgattga cctcgcccag
     3361 caccgcgacg agggttacat ctccctgaag gagatttccg ccaggcagga cgttaccgta
     3421 cgttatctgg aacagattat cgccatcctt ttgaaagcgg gattcgtcca gagcttccgg
     3481 ggaaaggccg gcggataccg cctttccagg catccgcgcg aatatactac ggaagatatc
     3541 ctgaaactga cggaaggctc cctcctgccc ctttcctgca cggcttccca cgagaatccg
     3601 tgcccgcggg cggccggctg cgccacgctg cctttctggc gcgggctgca acaagtcatt
     3661 gagaattatt tgcgtcgcgt aacgctggaa gatctggccg aacagcagaa ggaaatcggc
     3721 tgcgactacg gagcaggtat ttaagcagcg gctgcgggcc tgaacatttc cctttccttt
     3781 cccattccgg cccatgtccc gtttttttta cccggagctt gacatctgct ttccttttca
     3841 ttatttattc cctagttttc cgataggaat ataaaaacca tgagtaagaa ccatcgattt
     3901 gaaacacgcc agatccatgt gggtcaggaa agtccggatc cggccaccga tgcgcgcgcc
     3961 gtgcctattt acgccaccac ttcgtatgtc ttcaaagact cggaacaggc ggcaggccgt
     4021 tttgccctgg cggagccggg caatatttat aaccgcctga tgaaccctac cgcagatgtt
     4081 tttgaaaaac gcatcgcctc cctggaagga gggacggccg cgctggccgt ctccaccggg
     4141 gctgccgctg tcacgtatgc catccagaac atcgcccggg ccggggacca catcgtttct
     4201 tcttccaccg tatatggcgg gacgtataat ctctttgcca atacgctggc ggacgccggc
     4261 atagaaacca cttttgtgga tgcaagggac gttcagaatt tttccagggc catccggaac
     4321 aataccaagg ccctgtacgt ggaaagcctg ggcaacccga actgcgacat cgtggatatg
     4381 gaagcgctgg cggaagtggc gcacgcccac ggcatcccgc tcattgtgga cagcacgttc
     4441 gccacgccct tcctgttccg ccccctggaa cacggagcgg acatcgtggt gcattccgct
     4501 accaaattca tcggcggcca cggcacggtg atgggcggcg tgattgtgga cggcggtaaa
     4561 ttcgactgga cgcagaacga caagttcccc
//
LOCUS       DAWEMX010000163         4461 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7135_length_4461_cov_8.865411, whole genome shotgun sequence.
ACCESSION   DAWEMX010000163 DAWEMX010000000
VERSION     DAWEMX010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7135_length_4461_cov_8.865411"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          934 a         1424 c         1205 g          898 t
ORIGIN      
        1 atgaaaacgt gggcctcgtt caaacccgct ggggccacat caaccgtgaa tacactctcc
       61 tcccccgcat ccaggggatg tacctggacg gccattttgc catggaacag acggctcgca
      121 accgttccgg ccgcttcttc accttcaacg gcactgccgg catatggcgc aagtgcgtga
      181 tcggcgacgc gggcggatgg tcccatgaca ccctgacgga agacatggac ctttcctacc
      241 gtgtccagct caggggctgg cgcttcatct acctgaacga cgtcgttacc ccggcggaac
      301 ttcccgtgga catggacggg ttcaagtccc agcagcaccg gtggaccaag ggttccattc
      361 aggtctgcca gaaaatcctg ctggacatct ggcgctccaa tgcccccctt aaagccaagg
      421 tagaagccac cacccacctc acctgcaatt attcctacct gctcctggca ttgctctgct
      481 tcctggtgta ccccatctgc acgcagcgca ttccggaaaa tgaaacggtc ttcatgtggt
      541 ttgtgaatgt ggccctgttc ttcatgacca gcgtggccgt ctgcatcttt tacatgagtg
      601 cccaaatcgt ggtgcgtccc aaatcctggt ggaaggaact gccgtacctc cccctccttc
      661 tcacgctgag cgtgggcatg gcggtcaaca acgccaaggc tgttctggaa gccatcttcg
      721 gccatcagtc cgccttcgtc cgcacgccca aatacggcgt ggaccggaag gaagaccgcc
      781 ggcacgtctc ccagctcaaa cgcaacggat accgggctat taaatccgtc gtcattcccg
      841 tcctggaaat cgcctgcgga actttcttcc tgaacctgat catcatcatg atccgccagg
      901 gccattacct ttcccccatc ctgatgattc ctttcctggg tttctattac acagggttct
      961 gttccctggg ccgcatgatt tccaacctgc tgcccgccaa gcaaactgcc aaagccaaga
     1021 actgacgcct ttcccatgcc tgcgcctctc tcgcaagcag cacgcctgat tcaactcgcc
     1081 ggcgcagctt tcgctgtgtt cggagccatg gcatcctgtt cccgtccgga ttatgagccc
     1141 accgccatcg tgaaagacgg cgcggtcgtc agcgtcaggg accagaaaat ggctgtcatg
     1201 cgcaatggcc gggtggtgaa gacctaccct gtcagcactt ccaaattcgg cctgggagac
     1261 agaaagggaa gctgccgcac tcctctgggc gcccatcgca tcgcttccaa aatcggcagc
     1321 ggacaaccca agggcatggt gtttaaaagc cgcaagccca ccggggaatg cgtcgcgccc
     1381 aattctccgg gccgggaccc catcgtcacg cgcatcatgt ggctggaagg tctggaaccc
     1441 cgtaaccgga atgctcactc gcgcctgatt tatatccacg gtacggcgga ggaacgcacc
     1501 atcggcaccc ccgcctccta cggctgcgtc cggatgacga gcaatgacgt atatgaggcc
     1561 ttcaacctgc tccgtaccgg agacccggtc gtaatcgaac gctgttccat tgacgcgtcc
     1621 ctgaaagccc tggcccaggc ggaaaaaatg caggattctt ccctgctggt catggcttcc
     1681 gctccagacc gcatggaaaa cgccaatacg gcctccgtca tttcccaccg ccgcagggct
     1741 tcccgccccc ccgccgttgc cctttcccgc aagcaaacgg catcaaccgg gaaaaaatcc
     1801 gttccctcca ggaggacggt cgcccgcagg acgcccggaa attcaagaac cgccgtccgt
     1861 tcctccggag ggacccgcta tacactcggg taaaagctcc ccttccctcc tgtttttccg
     1921 gtgttccgtt ttcaccgcct cctgacatgg ccgtttgaac gcatggttgc ctgcatccgt
     1981 cacatttggt aacgtagatt tatggccatg atggaacacg aagatatcag caatgaaaac
     2041 ctggcggggc accttctggt tgccgccccc tacctggatg gcgccggatt ccatcattcc
     2101 gtcatcttcc tgagccgggc ggagaaggaa tttgtcatcg gccatatttt gaatcatccc
     2161 tccggcataa atgtggggga cgtagcccgc catacggaaa taccggaatc cctttacgcc
     2221 gtgcccatct tcaagggagg gcccgtagaa cgcaaccagc tcatcttcgc cgctttcatc
     2281 cgcacggagg ataaattgcg cgtccagttt catctgcaag aggaacaggc cctggaatac
     2341 ctggaagacc cccgcgccat cctgcgcgcc tacgtaggcc atagcggatg gacgccgccc
     2401 cagctgcgcc gggaactgaa tgaccgtgcc tggtatgttt cccccatggt gccggacatt
     2461 tgcctggaaa cggattcttc caaggtctgg gccatggcca tgcggcggct ctctcccctg
     2521 caccatatca tgagccacgc gtccgcacag ccatccctga actgaaccat ccatcatgag
     2581 catccaacag aatctggaac atattctctc ccgcatacgc gcagcggaac tccgcgccgg
     2641 acgtccggcg ggttccgtcc gcctcgtcgc cgtcagcaaa acttttcccg cagaggatgt
     2701 gaaagcgtgc catgatgcgg gccagcgcat tttcggtgaa aacaaggtcc aggagggact
     2761 gggaaaaatc ccctcccttc ctccggatac ggaatggcat ctcattggcc ccctccagcg
     2821 caacaaggtg cgcaaggccc tggaatattt cacgctgatc catgccgtgg actccctgcg
     2881 cttggcccgg ttcatggata ccgtggctca ggaaacggga aaacggcccc gcattttgct
     2941 ggaagtgaac gtaggcaatg aagacagcaa gttcggattc ttcccggaaa ccctggaaaa
     3001 ggaatggccg gagctgtcct ccctgaacca tcttgaaatc gccggcctta tgtgcattcc
     3061 ccccccggtg gaaaatccgg aggaggcgcg gccctatttc cgccgcctcc gggagctgaa
     3121 ggaagtcctg tccggcaggg gaggagccac gctgacggaa ctgtccatgg gcatgagcca
     3181 cgactttgaa acggcaatag aggaaggagc cacgctcgtc cgcgtaggga cggccatttt
     3241 cggcggccgc tcgtacgctc ccgcttcgtg attttagttg gacagaagga aaagcggcgt
     3301 gtaggtttgt tccggaaaac cggaacagcc cggacggaga cctgatttca tcaccccatg
     3361 ctgacaggag gaaaaaacgt gattccgcat gcggtgcggg gcgtagcgga tgccgccgcg
     3421 gcgttttccg cccgcatcgg ccccgaccgt catttcgatt cccaggaaaa ggtggaggaa
     3481 gccctggccg aatgctgctc tccgcagaat gtcaccgtca tcggcatgac agggtccggc
     3541 aaatcctcta cgctggacgc cctggccggg gaacggttct gttcccgcgt gtccagtgaa
     3601 gccacactgg tccgctggca gtacaggcct catcccgccc cacacaccca tgaatgggtt
     3661 ctggaccttt tttacccgtc ggacgccctg ctcaacctgg agttctggga taccatcggc
     3721 ctggaacagg aggatgcccc cagaaagctg aagcacatcg ttcccaaatc ggatgccatc
     3781 cttgtggtta tttccgccag tgacggcaac cattccgccc tgtgggacta tctccggacg
     3841 ctggaggaac gtctccattc ccgcatggtt ctcgtcatca cccatgcgga gctgctctcc
     3901 tttgagaagc ttcaatcctt gaaagaggag ctacgctcca cctcccggga atgcttgggc
     3961 gtgcagctgc ccctgtaccc tgtcacgacc gccggagagc atgcagggga aggagtggaa
     4021 gccctgacgg cgtgcgttca ggaagtgctg aaacgcagcc cgctgacgga caagcgcgtg
     4081 gaacgcctcc tgctggctac ggacagcctt ctggaagaac aggggaaggt gcttgatgaa
     4141 ctgagccgcc tttccaagat ggattccggc ttcctgacgt ctattgaccg ggaaatcgac
     4201 cgcatccagc tccagatgga ggatgaaatg ccggcacgcc tcaaggcgta cgcgcaatac
     4261 gtgcaggact gcgtgccgcg tctggggaaa aagtcggcac gccagatggg gcgtttccta
     4321 tccatccggc gcacgatgat cctgcacaag cttcctcccg tcattgacga atggttttat
     4381 gagctggtca aatccggcat tgaggaacgg cacgcgtatt acaacaagga attcctgaac
     4441 atctgccaga cgcattggaa c
//
LOCUS       DAWEMX010000164         4456 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7142_length_4456_cov_14.068848, whole genome shotgun sequence.
ACCESSION   DAWEMX010000164 DAWEMX010000000
VERSION     DAWEMX010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_4456_cov_14.068848"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1931..1940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          838 a         1311 c         1315 g          982 t
ORIGIN      
        1 ggtatcccgt gcgttgagcc tctggggcgg ggcatccagc aggtggaagc cttccgcgac
       61 aatgcgtgaa acaacttccg ccgtgacgcc tccccacgga aaatcttccc cgacgaccag
      121 gacgcggccg gtgcgcgcta cggaggcgat aacggtatcc atgtccagcg ggcgcaccgt
      181 ccgaagatcc acgacctcca cttcatagcc gctttccttc tcaagcaggt ccgccgcacg
      241 cacggcttcc ggcaccatgg cgctgtacgc caccacggtg gcatgcttcc ccgtgcgggc
      301 gatccgggcc gtgccgaacg ggatgaccgg ggcttcccgg taattgtcct ccgccttcag
      361 ccagcggtac aggaacttgt gctccagaaa aatgacgggg tccggaattt ccacggcctg
      421 gcgcagcatc cagtaagcat ccgctaccgt ggccggagtc atgacgtgca ggcccggata
      481 atgggcgaac aaggcttcca ggctctggct gtggaaaggg ccggtgccgg gggttccccc
      541 gcagggcagg cgcaccgtga tgttggcggg aatgcccgtg cggtagtaat gggtggccgc
      601 catgttgaca atctggttga aagcgatcgt gctgaagtcg gcaaactgga cttccataat
      661 gggcttcttg cccatgacgg cggccccggt caccatgccg gccatggcgt cctcactgat
      721 gggggcgtca atcacccggt ccgggaacag ctccttgagg cccttggtcg ctttgaatgc
      781 gccgccgaaa acgcctatgt cctgcccgta cagaaagacg tccctgtctt ccgtaagcag
      841 gtctttctgg gcgtcgtgga tggcatcaat gtatgttacg ctcatgggaa tattccgttt
      901 gctggcttaa taaggtctcc ataccgtagc gttccagtct tcccggaagg ggtccggttc
      961 cggttcccgc tgcgcggtgg ctacggccag ctggacttca tcggcatact gttttttcaa
     1021 agccgccgtt tcttccggcg tgagccagcc ggcttccagc agctgccgtt ccgcgactgc
     1081 cacggggtct tttttttcat attcctcttt cagctccctg gggatgtagg aggcgtcgtc
     1141 atgttccccg tgcccgcaca tgcggagggt cttggcaagc acccactggg ggccccggcc
     1201 ttccctcgcg ttattgacgg cggtccggaa ggtttccagc gtggccatga agtccgtgcc
     1261 gtccgtttca tgcacggtga atccgtagcc gcggcccctg tccgccaggg aggcttctcc
     1321 gaattccctg atattggggg tagagtaggc aaattgatta ttggttacca cgagcaccag
     1381 aggcagccgc tccacgttcg ccatattggc ggcttcgtga aaagctcccg tggacgtggt
     1441 gccgtcgccg cagaaagcta ctcccacggg gccgggcagt ttgccgtcca ggcgcttggc
     1501 gaaaaggcag ccgatgacga aagccaccgt gctgcccaga tggctgatgg gagccatata
     1561 gccttccgct ggcaggcccc ggtggacatt gccgtcccgc cctttcatat atcccagggc
     1621 ggaacccagg taggcgcgtg ccgcttcaat aatcggttcc ccccaggtga cgcgtgccgc
     1681 ctgttcccgg atgaacgggg cgattacatc gtagccggca gtcagaaaga cgcctccgca
     1741 tgcggcaatc gcttcatgtc cgcggcccag gtaaacgccc cccgtaattt tgcccgcttt
     1801 gtaaaggctg gaaattttgg tgtcaaaagc ccgcgccagc aacatggcct tgaaagccct
     1861 gacggcattt tcctttaagt ccggttgtgt agtgtcgcaa tgtgccaccc cattatttta
     1921 tatgctgcat nnnnnnnnnn ctgcataagg aaatgcaatg ccatttcatg aaactgcctg
     1981 agactttgtg tccgcaggcg cggggccgct ccgcagggcg gcggaggttc gcggggggcg
     2041 gttcccggct ggcgggagcg cttttgcatc ttccgctccc gccggcggat gcgcggaaat
     2101 ttccggattc atttcccacc cggcggcagg ctgcatctca gttcgaattt gggatttgcc
     2161 acatcgtaat ctccgggaaa caattccgtc tccataatga ttttatcata ttcctgcgca
     2221 aaaaggtccg gaaaaacgcc gggcgcctcc gcaatgccgc tttcctgcct gatgacgcag
     2281 agaaaggagg cggcgggcag gcgcatgatt tccttcatcc gccgggcgtc tctgccggcc
     2341 gcttccacct ccacatacag gaactgctcc gtccctgctt tccggtggaa ggaaagcagg
     2401 ccggcttccg cgcccagccg cagctggcag cggttaatat cgtcaaagat gcgggtgata
     2461 agaatgttaa attccggagt atgctgtttt cccgcatagg gcgccaccca gcagtatttt
     2521 tccggcaatg cgtggatggc ctgttcccca tgaagcagaa gatcttcttc ccggcccatc
     2581 tgtttccgga atttttccag caggcgcagc ttttcctgaa tatccctgat tttccggtgt
     2641 gccagcatgg tgccgtggga gaaaagtttc ttataatgga tccttcgctg gtccctggtc
     2701 aggaagccgg taaattcctt caggggaata tccaggaaaa tgcaggcgcg gatggcatcc
     2761 accacgggaa tctgggaaag agtgtagtaa cggtagccgg tgtccgggtc cgtataggcc
     2821 gggagcaaaa tgcccagttg ttcataatac cggagggatt tgacatgtac gccggtctgt
     2881 ttggacagat tgccgatggt tagtaaattc tgtttgtcca tcctgtattg cggttggtgg
     2941 gagggaattg attgtttccg ccggaagagc tccggcggaa acgggagctg cgcgcagaag
     3001 aaacctttct gaagacgaag ttattttcgc tattcattgg atttttattg tcaacaattt
     3061 acaataaatg acaaagacct tccccactta atttttccat tgcctcttga ctctcccatt
     3121 atgggatact ttacatcata tggcaattga aaaatccccg gtcttgccgg aagtgcggtt
     3181 ccgggatttc ttgaggttcc aaacagcatg attcctgatg aaaataatta atcttttatt
     3241 ctgcatgccc ctgctggtgg tttgcttctc ctgccagcgg gagggtgaaa gccaggcagc
     3301 cggattgccc ggcgaaaccg tgccggaagt gtctgtcatg acggtacgca gtactcaggt
     3361 tcccgtaacc gccaatcttc ccggccgtat ggaagcgtat ctccaggcgg aagtgcgcgc
     3421 ccgcgtgacg ggaattattc aggaacgctg ctatcaggaa gggcagacgg tccggccggg
     3481 cgatcttctt tttaaaatag acccggctcc gcttcaggct gtcctggatg aatgcaaggc
     3541 tgcagtggct cgtgccaggg ccgttctgtc ggatgcggag gacaaggccg cccggtattc
     3601 ctccctggtc gccaagggcg ccgtcagcat acgtgagcat aagcaggcca gggcggagga
     3661 agagcgggcc agggcggaat atgccgccgc ggccgcctct ctggagcagg cgcgcctgaa
     3721 tctggaatat acccgggtgg aggcgcccat ctccggccgc gtgcgccgtg ccctggtgac
     3781 ggaaggggcg tttgccaacc agaatgaatt cacccacctg accaccattg aacagattga
     3841 ccccatttat gtccgcttca gccagccggc ctcccagtac agcagcctgc gccgcgccgt
     3901 cgtatccgga ttgtggaagg gtgtgccgct gggcgaaatc aaggtgcgcc tgctgctctc
     3961 caatggggag gaataccccc attccggcag gatcattttt tccgacatgg cggtggaccc
     4021 gaatacggat actattgaaa tgcgggccct gttccccaac ccggaccatg aactgctgcc
     4081 cggcgcctat gtgcgcgtgg tatttgacag ggcggtgcgg gacaacgtct tcgccattcc
     4141 ccgggatgcc gtcatccgca ccgcgcaggg cgcttccgtc tttgtcgtgg gccctgaagg
     4201 tgtgctggaa gcgcgccccg tcagggcgga tacgctgaac ggccgggaat ggctggtttc
     4261 agaaggcctc cgggatgggg acagggttgc cgtcagccac acgatgtccc tccgccccgg
     4321 catgaaagtc cgcagcgccg ccgcccggcc gcaaccccat gcccagcaat aaaccgctat
     4381 gcctgatttc ttcatacgcc gccccatttt tgcctgggta gtcgcactcc tgatttccct
     4441 gatgggcctt ctggcc
//
LOCUS       DAWEMX010000165         4359 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7311_length_4359_cov_10.165195, whole genome shotgun sequence.
ACCESSION   DAWEMX010000165 DAWEMX010000000
VERSION     DAWEMX010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7311_length_4359_cov_10.165195"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    771..780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1019 a         1372 c         1136 g          822 t
ORIGIN      
        1 atccggaacc attcagcaaa ccccatgccg agttcatgcg ccgcaatggc attgggagga
       61 gaccccacca gcgtccccat accgccgatg gaggcgctgt acgccacacc cagaatagca
      121 aaaggagccg tcgtcttaag tttttccgcc ggaatgcggt ccagaaggcc gatcaccaga
      181 ggaagcatca tcgccgccgt ggccgtattg gacatccaca tggaaagaaa cgccgtagcc
      241 aggaaaatca gaatcagagc catccccaaa ctcccgcggg ccattcggag gatttttccc
      301 gccagccagg tatcaatttt ctgttcgtgg agagccccgg ccagggcaaa gcccccgaaa
      361 aacagaaaaa tggtaggatc cgcaaaaccg gacagggcct tcgcccccgg cagaatcccc
      421 atcaacatgg caagcaccgg caccagcagg gaggttacgg ttacgtgcag ggcttccgtc
      481 agccacagga tgccgataaa ggtaagaaca gcaagccccc tggccacctc cggctctacg
      541 ggaagccact tgagcatagc caggaaaaga agcacgtcac acgcaatgat gatgtaattg
      601 cggtgccctt tgggagtgtc tgtagtacgg tggcgcataa agaaaagata aacgacgcta
      661 ttttctttgt acgcatcaga aaaacagtgt caatgctttt ccataaaaat cccggaaaca
      721 gggaaacacg gaccagcgca tcggcttgag gatacgcacg gcacgcatta nnnnnnnnnn
      781 taccggaaaa gcagcgcctc ttcttcctgc ttctgaaaaa ttcaggaagt ccggacacgc
      841 aaaaaaccgc catgcaatcg cacggcggct tgtaacaact gtttgactgg cgcgcggagg
      901 ggcagcctcc gcccgggcaa ttattcggcg gcgggttcag cggccttctt ggcagccggc
      961 tttttagcgg gggccttctt ggcggcggca gccttggttt cagtcttttt ggccggggcc
     1021 ttggccgcag tcttcttggc gggagcttcc ttggcggcag gcttggcatc cttggcaacg
     1081 ggaacggcca gacggatagg cgcattggaa ttcaacttgg actgttcctt cttacgcttg
     1141 aggtattcaa tacgtctgcg gcgcttggtg actttgcgga tttgctgtcc catagtgata
     1201 attgctatga tatttactaa ttcttacgag ctccatgtgg cacatgggcg tggatacaac
     1261 taccaacccc acctccctta cgcaagcaca aagcgttatt aaggcacaga aaggcggagg
     1321 cagtccccgg ttaccccaac ccgcgcggaa gaggaaaaca acttggatga aggaggcttt
     1381 accacaattt cagcgcatcc acccggaccg gctgttccca gcgcagatgg ttccggattg
     1441 cggaagcgat gaattccagg ccgcgctcca ccgccgcaac cagtccatcc ccggcagcca
     1501 gccgtgccgc gacggcggcg gacagggaac agcccgttcc gtgagtactc acatcctgta
     1561 cgcgcggacg ctcccattgc cccagcaggc agccgtccgg gccggacagc acgtcccggc
     1621 agtccccttc cagatgcccg cccttcagaa gaacggggca tccgtacctg acagcgaggc
     1681 gcgcggcggc ttccggcagt tcgtcccttc ccggattaac ggaagagcgg agcaatacgg
     1741 cagcctcatc cagattgggc gtaagcaatg ccgcacccgg cagcagaagc tcttcataaa
     1801 cagccaccgc ttcttcccgc atcaggcggt cgcccgccgt ggcgatcatg accgggtcca
     1861 ccacgacggg aatatccgcc ccgtccagca ccccatacac cgcacggacg atagccgggg
     1921 aatacagcat ccccgtcttc acggcgcgca cgggaaaatg ttccagattg atgcgcacct
     1981 gatcggccac cagcgaaggg tccacctcct gaatgccgcg cacagtcccc ggcgcctcgg
     2041 acacgacgca ggtgacggcc gtaagcgcaa acgcccccat ggcatgggcc gctttcaggt
     2101 cagcctggag cccggccccc gcggagcaat cggaaccggc aatcgtcatc attacgggaa
     2161 tactcatggc cttgttttta atcgggaagg aagcccttga acatcccaaa atccgcatgt
     2221 gccgcctcaa tgtcacaaat ccgggtggga aaacaaggag ggaatgccac aatcaaccca
     2281 tgacgcacgc ctccccagcc ccggatgaac aggaattctg gcttttcatt caggatgccc
     2341 ctctgccctc cgccgaggaa ttgcagaaga acatgttccg cctggacggc tgtttccggc
     2401 tgaacctttc cggtgaaccg gaaaacgctc cggaccatgt gctggaagga gaatatctgg
     2461 atgaagaggg agaatcccag ctggacgtat tctgggtcat ggaaacggac gacgcgcgcc
     2521 aggctttcaa aaaggacggc gcggctctcc ggcagatagg gaaccgcacc aaaatgctgc
     2581 gcttcattct ggaagacgaa tatgccctgg gccatgtatt cgccatgatt cacgccatac
     2641 tggaacaccg ggacggcctg ctggttattc cggacacccg gggaaacatt attctggaac
     2701 ggaggaaagc tttggatttt ctgaacaggg aaattcggga aagttaacgt ttttgcgttt
     2761 gtagcgcagg gcttccctcg cgttccgcat tcagttgaag cgccttcttt ttccagactt
     2821 ccgccatatc cgcatcctga ggggtcccct ttcctgtgct gtagcagacg gaaagcatca
     2881 gcatggcgtt gacgtggtcc tgcccggcgg ccagccggag ccagctgaac gccttgccct
     2941 catccacagg cacgccggaa ccttccagac aggccagtcc cacaatatac tgggaatggg
     3001 cgtctccggc tgaagccgcc tgttcatacc acttcatggc acgctccata tcccgctcca
     3061 ccccctgccc ctttgcataa atgttcccca gatagacgga cgaaggcgta ttccccccgt
     3121 tggcggcctt ctccagcagc gccaggccgg aagcggcatc cttctcaaac cccatcttcc
     3181 cctccatgta agcccggccc agcagcccca tggctgccgg atgttcctca tccgccgcct
     3241 tgtgccacag ccccagcgcc ttattcaggt cctgatccgc gccatccccc ttgaaataca
     3301 tcattcccaa catgtacagg gcgtttcccc gctgggtccc cgggcgggca agcccgtact
     3361 caaaccactc cctggccgct acggcatcac gcttgattcc cgtcccatac aggaacatgg
     3421 cgccgatgac cgtttccccg gaatacccgg aaccggtctc cgccacggaa tcacaccagg
     3481 cccgcacatc cagcggatga acccagccat tctgcaatcc ctccgcatac agggcagcca
     3541 catcattgag ggctttcaca tgctcgctac cattttcctt ctcgcggacg gagccggcaa
     3601 cagcagcgtc cagacgctct ctccatgtct ccgcctcttt ccgcaccgca gacggctttt
     3661 cccgttccgc aaaagaagca gcccgctttt ccccggaaga aggggcgcgg tcacatccgc
     3721 acaaaaaaaa gaaggcggag gaagccgcac ataaagagac aacttttttc atcatggcca
     3781 ttaccataag acagcctcat caggctgcca agaagagaaa tgacggaaac gccgcatccg
     3841 cacgctatgg aagagcgaaa ccgaagagcc ggattccctc tttttgcatt gaatattttc
     3901 ctctttttgc tctactgaaa ccagttcaac ctctgatgaa attcgttccg cccctgtatc
     3961 tcttgacatg cctgggcctg gcgttcctgg gaagtttttc cagccggggt tccgcaacgg
     4021 aacttcccgt aaaatacatt tttgagctgt cggatgaacc ggtgaaagtg caccctggca
     4081 aaatagacac caagagcgtt ttcttcccca aatacgcacg cggaacctct ccggaaaccc
     4141 tgaagcggca ggcagccctc ttccaatccc atactcccca cgaggccacc aaaagcaaac
     4201 cggccatcag catccacatg aatgacgaca aaacggaaag ggacgtccat gagcggtcag
     4261 gctatgagtt tcaccatgcg gaaaaccagg aagaaattgt catcacaacc gaaacgccgg
     4321 atgaggaaat ctggctgaac gacggggatc cggacagcc
//
LOCUS       DAWEMX010000166         4303 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7389_length_4303_cov_12.938089, whole genome shotgun sequence.
ACCESSION   DAWEMX010000166 DAWEMX010000000
VERSION     DAWEMX010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7389_length_4303_cov_12.938089"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3123..3132
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1037 a         1215 c         1193 g          848 t
ORIGIN      
        1 ggtcagaaga acggaggcgg acatggcatc cgcccccagg cccacccttc ccgcactgga
       61 gcctgacaac cctgccagca tcccggaagc caacatcacc atccaggaac cggaggaaac
      121 gcaagaatcc tccctccatg ccatgatgaa agcctggatg accttcgccc tggccaatgt
      181 agccggcatc atcctgcaca tctccgcact ggcggccata gtggtgctct ggctggtctg
      241 gaaaaacaag agagtggtgc atctgctgcc gtcagagccg gacaagcgcc tgggcgctcc
      301 ccacggcgcc tcccagagcc gccccgtgaa ctactgcatg caggacagcc gcgggccgaa
      361 ctccatcgga cgcaggcaaa tgcgccagca catgcgcgac gtctcatgac agaatcatcc
      421 tcaccccccg gaatccgcgc ctccctgaat gaggcgggag aactggtact taccgcggtg
      481 aacgctccgc cggaagccgt catccgaatg gacgtgaatg cgggctctcc ccgcatgctc
      541 tgcacgcagg gccgctatct ggctccggtg caggccccgc ccggcgcgcg catccgcttc
      601 cgcctgttcc ggggaaagcg cggcattaca gccccggaaa ccttcatcat gcccggacct
      661 cctcccgccc gggccgtacc ctccacgctg attccctgca cccaggaccg ggacttcatg
      721 atttacgact gggcgtcccg gcatgaagcc gcgtgccgca tcgtgcgcga aacacacccg
      781 gacctcctct tcatcggaga ctccatcacg catttctggg gaggtgcgcc cgtggatgaa
      841 ccgcaccggg acattttgca aaaatccccg gaaacatgga acctgtgcac ggcgggaatg
      901 cgggcggtca atctggggtt cggctacgac cgggtggaaa acgccctgtg gcggctccgc
      961 cacggagaac tggacggtgc ggaagacaat gccgtttgcg tagtgctcct gggcacgaac
     1021 aaccttgcgg aaaacacgga cggggaaatc ctggaaggca ttcgtgccgt ctgccgggaa
     1081 atcaccggca aactggcaaa agccgtcatc atcctccagg gattttaccc gcgcaacagc
     1141 gcccgggaag gtacggcgga acgcattgcc ggcatcaatc tcctcctgaa tcgcctggcc
     1201 gcggaagaaa atttcattta tacggaaccg ggacgcgtca tggctaatag cgacggatac
     1261 gtcccggaag agctttccag cgacggactg cacccttctg cggccggtta cgcacgcatc
     1321 gccgccgtgc tggcccccgt catcagacag gcggcagaac gggaaaaatg acaccggcaa
     1381 gaaaccggac ccatcttccg gtgtttttct tcatagaacc ctcagcggaa ccacaaccat
     1441 gaacgaacag gacgatacaa aactctggca gattctggga catgcctccc aacctgcgcc
     1501 gggggacgat tttgcccgca aggtcatgat gcgtattgaa agggaagaat gcgctcccgc
     1561 cgttccggtg gaatccattc atcacttcaa gcggcattcc ttccgcattt ggggagctgc
     1621 tgcggcggcc ctggtggctg ccgtcatcgg cattgcagcc ctgatggaac cttctgcccc
     1681 ggaaacggct cccctttcca tcgccacctt gaatattgac gatgtgctgg tggaggaagc
     1741 cgggctggct ctggggcagg aaaacctggt agacgccctc tgcgtactgt cctccacgga
     1801 aacgggcgtc atctcctcgg acaacatcca ggaccttctt ctgtaactcc gggaacggaa
     1861 ataccattca atgcccgtcc ggcgtcacaa gcctccgtgc cgggcgtttc cgtattattt
     1921 ttcccgactg ccttgaatcc gtatttcaga aaccccgctt tttccgaaaa cggaaatgta
     1981 aagagtctgt atcccggagg ggcaactcat ccggcaggag gaacggggct accagcccct
     2041 gtagggtcta gcttaattca tacacaagcg tagcgatacg ttccaaacta ttaacatata
     2101 tatggcgggc gctggatggc atccgccatt tttattggag ataatcgggg attttccccc
     2161 atcacgtccg gggcgtgttg ttgttcttcc tggaattcct tatgttcatt tgagagctaa
     2221 atggttagtt ctcaaacaag aaacaattca ggaacatgaa taattatata ccactgaaca
     2281 aggcggtcgg tccgaagctt atctgcgacg gggagcaggc tctcatcccg cagaaactcg
     2341 gagttgacgc gatggagttc aactttgacg gttcggcgtg cgaacacgcc acttgcgccg
     2401 gagcgccgga aatgttcatc aaggtcgagg aagacggcct gtattatttc ggcgtagagg
     2461 ccgacgacac aggcagcatt tccattgcgg gcgacgtgct ttgcaagaag gatgggacac
     2521 ctcccaacgg caagctgaat attcaaacgg aaatgatagg attgaaagct ggctattaca
     2581 aggtggcctt gtcttacacg aataatgcct acacccccgt cagcaacaac gctgccgcgt
     2641 tcaatgtgac gatggacagg gaacctatcc aggcagggaa gtacgaaggc aactcgacga
     2701 tgaagcgcga attctcgcct tctcccaaaa tcaagctgtg gacgattgaa aaggaatcca
     2761 ccatcacctg tgaagagtcc aagaaagtgg aggtgacgct tgaagaacct gcgcctgtcg
     2821 ttgaggaaag tatcggagat actaaaacgt cccttatcag tccccagatc atcgtaacgg
     2881 cttgcaagga tgaagtgctc gacgagtgga gactgcgcgt gcagcaagtt tccgccggtt
     2941 ccaggattct cttgcgcacg ggaggataca gggacgccct taaaaatccc cctgtcaccg
     3001 aagatgaagc cgtcgaagcg gtcactgcaa tgaaccgtta ccagtctcat aaacgcggcg
     3061 catggcatac tcaagaggca tccctcgccc acgagaaaca ccaccgccga gaatgcaatg
     3121 acnnnnnnnn nntattttga agctgtctat aactatatca aagtattgcc ggatgaggcc
     3181 aatgaccgtc cctactgtgc cggacaaaaa gttctgaacg aggcaacccg gaagattatt
     3241 gcccaggcaa aggccaatgg gtggagcaga gtacccgatg aagtcacgga accgggaacg
     3301 attgaaccgc cctgtttcct tccgcccgtc aacggcatgt atgcccgcag cgtggctgct
     3361 gcggaggaac cggcgccctt gaccctctcc atggcggata cctcccggtt cagggaaggc
     3421 agaatcacag tccgcttccg caatgaagga aacgaacctg tccggattcc ggacgaaatc
     3481 aacgatgaga cggctgatta tttcttcctg acggtgttga ggacggaacg ggggaacttc
     3541 cgcgtcctga acaggacagg cgggaaaata accttcaatc gttccttgaa ctaccgggaa
     3601 ctggcccccg gccgggaata cagcgtcacg attccggtgt gtctggatga agtcgatctg
     3661 gaaggctgga aacaatgttc ttgtgaactc gaaacgcgct actataatca gcagggtaag
     3721 gattgtttcc taggggttct ccgggcaacg gccaggctca cgctgcaatg aaaaaaatct
     3781 tccggtattt aatacttctg gcaagcctgt ccctccatgc tgcggcatgg ggggacatgg
     3841 ccgacaacgg agtggaatgc tccgtggtga tggaaaagac ccggtttgac gcgagggaga
     3901 cgttccctga cttcaaactg gtgttcacga acaagggcga aaagaccgtg cgcctgttcg
     3961 acgacttcta tcccctcaag gatgacggcc ctaatatccg tatcgaaata tggaaaaaag
     4021 cgggagggaa aagatatggg tacagagtgg gcgaaataga gaatccaata gcctgttatg
     4081 gagccggtta taaaatcgaa cgggtagcgg ctttgatgcg cttcattacg ttgaagccgg
     4141 gagaaaaaca tgaagtcccc atcaaggaag cttacctgtt gctgatgtat tttgaccgtc
     4201 ttcgcaatgg tcagaactat gagctggaag tacgtttcag ggatgaaagt tcgtatcccg
     4261 atgctcggag ggtgtatgta gggaaggcag aatttagttt gtc
//
LOCUS       DAWEMX010000167         4268 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7455_length_4268_cov_9.507477, whole genome shotgun sequence.
ACCESSION   DAWEMX010000167 DAWEMX010000000
VERSION     DAWEMX010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_4268_cov_9.507477"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1246..1255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          845 a         1159 c         1284 g          970 t
ORIGIN      
        1 tagtcattcc atgccttggc ggaatggaag cggttgcgtt cgtccggcga cagggctttg
       61 tcaattccgg aaaggaagtt catgggataa gttttccgca gggccgcgta actggtcact
      121 ttcgggttgc ggtccaccac ggaacgctgg tccgcaggat ggggaacctg gcccgtgagc
      181 aattcgtaga acgtggcgcc cagttcgtac atatcgcacc agggacctat ttcgctccgg
      241 cgtccggggt agaattccgg cggggtatag ccgggcgtga tctggacgag cccctgatgc
      301 tggagggtct tggtgcgcac ggcgccgaag tcgatgagct tgggcgttcc gtccggctgg
      361 atgaagatgt tgctcggctt gatgtcgcaa tggtacacac ccatggagtg aaggtagtgc
      421 aggatgtcca gcaggcggaa cagaagatcc gtggcctggg ccggttccat ggtttgcccg
      481 gtggcgtgca tggtggtcat caggtcgaac agggtctgtc cctggatatt ctccatgacg
      541 taatagcagg tgccgttggc gtcaaacatg tcaaacacct ggacaatgcc gggatggttg
      601 agctgggaca ggacggaagc ctcctgccgg gaattggaaa cgctctggga aaaattgacg
      661 gcggtctgtt cgttccgggg atggacaacg ccggtttcca tatcccggca ggcgtaggcg
      721 tccggcatgc attccttgat gacgacatac cgggaaccgg ggttgtccag agccaggtac
      781 gtgatgccga agccgccggc gttcagcacg ctcaatatcg tatatttgtc ttgcagaaca
      841 gtattgtccg caagcgggta aaccagtgtt tcttgttcca tgtcaggggg aagggcgggt
      901 tggagccggg aaaatcttta atttaatacg caagttctgt caagaatctc tccgccgcat
      961 tcagtaacag gagacgcgga attcatattc ttccacgctg ccgcgttcca ggcaggcggc
     1021 cagccagtcg aacagggctc cgaattcccc gcccagggtt tcccccgtat ctgcctccag
     1081 cgtcttcagc cttccggcgg ccagccgtac gggcgtgagc tccgccagca gggcggcgca
     1141 ttcttcatct ccggctttga aaatttcctc cgcgccgggg agctctccca ggcgttcgtc
     1201 cgcaatttcc aggcaaagca cccctacgcc gaactcggcg gccagnnnnn nnnnncccgc
     1261 ggagggcggc gcattcggaa tcatccggca gttcccccac aatgaccagt tctccgcact
     1321 gtgcccagcg ggacgtggcc agagtcctga aaaattccct tctgccgtct tctccaccgg
     1381 gcatgcgggc tacgcagacg ctgcggatgc ccatcatggg tgccccgatc atgcggcggc
     1441 gctgaagtgc gcttttgtca aaaacaagcg cgtttccctc ccattccccc tccggccatt
     1501 ccacaacggc cagatccggc tttacccagg aagttccggt ttcactggta ctgaacacga
     1561 aaacgccgcg gcccgtggta tcgtactggc gcacggccag ggccagcgcc ttggcgcggc
     1621 agttcagggc gtccggttcg ttttccccga ggaacagggg caaaagccct ctgggaacgc
     1681 gctgcccgtc ccgttccctc atgcgcctgt aatagccctg gccgcgttcc accttggcga
     1741 tttccgaatc cggctcggaa gccatgaaag aaaaatggta gcgcagggat gcgtccgaat
     1801 attcctcccc cagcctcagg cgcaccaggc ggatgagttc tgttccctta atggcctggg
     1861 ccggatcgga aggcaaaagt tccgggagca gttcttccag ctgggatcgt aaactcatac
     1921 gcgctccatg agaacacggg agaagcgctt cttcaaggcg gaaggagttc agcacatgaa
     1981 ataaaaagct cttttatttg ctccggaata cggcaagatg atccgcatgg catggattac
     2041 ctccggttat ataccttcgc ccgggcgcat ggccgtaatc gccggtacca cctttacgca
     2101 gctggtgcgc atgaaggtgt ttctctttct tgggctcttc gccgtggcgc tgctggctct
     2161 gagttccttc cgcctgagcg cggtgctggg gcctgaaacc ggggggatca atgaactcgt
     2221 gctgctgaaa aactccgcct acggcgccat gcgggtgttc ggcctctttt tctgcgtggc
     2281 ggccacggcc ctgattattc ccagggatgc ggaggaccgt attctataca ctatcctttg
     2341 caaaccggtg ccgcgcatcg actatctgat ggggaaggtg ctgggggtgc tggccctgac
     2401 cctggttgcc gttctgctga tggatgccgt aatgacgctg gtgctgtgga tgcggacgga
     2461 tacggtggtg gcggagcaga tagcctcgct gaaaggccgt tatacgttgg aggaaatgca
     2521 gccgtacctg gacaggatcc gcctccaggg agctacctgg aacgtgcaga cggggctggg
     2581 cgtgatgatg tgcgagtttg tcgtgctttc ctccctcacg ctgttgatgt cctgcgtgac
     2641 gaacggcacc attatcagcg cgctgctgac gttcatgatc tatctggcgg gactgttcca
     2701 gacgcaggct ctttccctgt gggtgggatc cggcacggat ggcctttcct ggtgggaggt
     2761 ggcgggcagc aggctctttt ccctggtttt tccgaatttc cagatttatt ccgtgacgga
     2821 ttccgccctg aacggacggc ttattcccct ttccctgttc ggaaccctgg ccctgattac
     2881 gctggggtac ttcgtctttc acatgacgct ggcggcgtgg ctgttcagga aaaaggaatt
     2941 ctaaggcgca accgtatgaa ggatatcatc aatgcgcgtt gcggctgggc cggaaccgat
     3001 gaactttaca taaaatacca tgatgaagaa tggggcaggc ctgtttccga tgacaaaacc
     3061 ctgtttgaat ttctggtgct ggagagcgcc caggcgggat tatcctggct tactatcctc
     3121 cggaagcgcg aaggataccg gagagccttc catggctttg atgtggagaa agtggcgcgg
     3181 atgacggcag aagatgtgga acggttgatg cgatttgatg gaattgtcaa aaaccgtttg
     3241 aaaatcaaca gcgctgtcaa taatgcgaaa ctgtttatgg ctgttcagga agaattcggc
     3301 agtttttacc ggtacgtctt gtcgtttttc cccaatcatc agcccgttgt taaccatttc
     3361 agcgctctgg ctcagattcc cgctgtttct ccggagtcgg aagccatgag cagggacatg
     3421 aaaaggcggg gattcatgtt tttcggtccg acgatctgtt atgcgttttt ccaggcaacc
     3481 gggtttgtca acgaccatat tgagggctgt ttctgcaacg cggcccagta cgcaaaatcc
     3541 caaccgcatg tccagccaga gacgttgata acagggccgg agggaacaaa cgcggaaatg
     3601 aggcattctc gtccatagtc atgggcctta attggcattt ccattttcta taaatcgctt
     3661 atatgttgac tgtaaaaaat catcaaccat gaaaacaggt atcatcggac ttggaaaaat
     3721 gggaggcgcc ctgttgcggg gcatgctgaa agccggagca gcggttccgg aggaagtttg
     3781 ggtgtacgat catcatcatg aaaacgtgca ggccctgcag gaggaatacc ccggagttca
     3841 tgaagctgcc tcggaggcgg atgcggcgga cgccgtggaa gtcctgattc tggccgtgaa
     3901 gccgcacgga gtccttccgc tcatttcctc tctttccgaa cggggggcgg agcttcccct
     3961 tctgatttct atcgctgccg ccgtttccct ggatgatatg gaagcctgcg ccagcgacgg
     4021 aacgcgcatt atacgcgcca tgcccaatac cccgtgcatg gtgctggccg gcgtcatcgc
     4081 ctacagtacg ggaaacggcg taaaagatga agatgaagag gctgcccgga tgctgctttc
     4141 cggctgcggc tccgtttaca aggtggcgga atccgggatg aatgccgttt ccgccatttc
     4201 cggttgcggc cctgcctaca tgtttacggc tctggatgcg cttgcggacg caggggtagc
     4261 catgggat
//
LOCUS       DAWEMX010000168         4257 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7473_length_4257_cov_10.552594, whole genome shotgun sequence.
ACCESSION   DAWEMX010000168 DAWEMX010000000
VERSION     DAWEMX010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7473_length_4257_cov_10.552594"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1342..1351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          965 a         1161 c         1107 g         1014 t
ORIGIN      
        1 ttttgaacgt tcctctcttc cctgctcttg tacaccacct ttcttcgaga aaaactcaga
       61 caatctccgc cacttgcaag gaagcctcga ttccgtagcc gggttcccaa actgatgaag
      121 gcagccctga tatgccgagt tctttccaca atttctactt cgttatgccg gaatattccg
      181 gctcagcgca gatttgaacg gaaaccgtgc ctattccgag aagaagaaat aggagtgcgc
      241 agattccata cgcggccttt ccgcaccatt ggagcaataa acagggacgc agctaacgcc
      301 gcgcccctgt ccgctcccgg caacacgccg gcaagcatgc ttttcagtta ttttttctcc
      361 ttggtgaagt ccagtttgcc gcctttttcc cgcaccacga tttccgttcc ttccggaaag
      421 tctccttcca gaagtttaag gctgagcggg tccagaagat catgctggat ggcccgcttg
      481 agaggccggg ctccataaac aggatcaaaa ccgtggtccg ccaccaaagc cgtagcttcc
      541 ggagtcatgc gcaaaaacag gcctttggct tccaggcgcc tgcggacacg ctgaagctga
      601 atgtccacaa tgcccttgag ttcctgcgcc gtgagccggt caaagatgat gatttcatca
      661 atgcggttca ggaattccgg acggaagtgg cctcgcaacg cctccagggc cttggcttcc
      721 cgctgttccg cattggcttc attgaggata tactggctgc cgatgttgga ggtcatgatg
      781 atgaccgtgt tgcggaagtc aaccgtacgc ccctggccgt ccgtaatacg gccgtcgtcc
      841 aatacctgga gcaatacgtt gaagacgtcc gggtgggcct tttcaatttc atcgaagaga
      901 accacgcagt acggacggcg ccgcacggct tcagagagct gaccgccttc ttcatacccc
      961 acgtatccgg gaggcgcccc gatgaggcgc gcaacagaat gtttttccat gtattcggac
     1021 atgtcaatgc ggacgatggc gttttcattg tcaaaaagga attccgccag cgccttgctg
     1081 agctccgtct tgccgacgcc agtgggtccc aggaacagga aagagccaat gggcctgttt
     1141 tcatcctgca acccggcgcg ggcgcgcctc acggcgtcgg atacggcctt aacggcctgt
     1201 ttctgcccga ttacgcgggc gcccaagcgt tcttccatgt gcaccagctt ggcacgctcc
     1261 ccttcctgca gacgggcagc gggaatgccg gtccaggtgg caaccacttt ggcgatgtct
     1321 ccttccgtga cttcctcctt cnnnnnnnnn ntccttgcct tccgccaggg cgcgctgggc
     1381 ctccggaagt tctccatagg tgatttccga tgcgcgggtc agatcaccca tccgtttggc
     1441 ccgttcactt tccagcttga gggcttctat tttttcctgt tccctgcgga cggcgtccag
     1501 cacttccttt tcacttttcc atttggcaat catggaaccg gatttttcct tcaaatcggc
     1561 caggtccttg gtgatttttt ccaaacgcgc cttgctgtcg gcgtcttctt ccttcgccag
     1621 tgcctgccgc tccatttcaa gctgcatggc ttcacgttca atctggtcaa tttccgtggg
     1681 catggagtcc agctcaattt tgaggcgggc tgccgcttca tccaccaggt ccactgcctt
     1741 gtcaggcaga aaacggtcgg aaatataacg gtcggaaagg gtggcggccg ccacgatggc
     1801 agcgtccgta atacgcaccc catggtgaac ttcatagcgt tctttcaggc cgcgcaggat
     1861 ggcaatggta tcttccacgc tgggctcgga gaccatgacg ggctgaaacc ggcgttccag
     1921 agccgcatcc ttttcaatgt atttgcggta ttcgtccagt gtcgtcgcgc cgatggtccg
     1981 cagttccccg cgggccaggg cgggtttgag caggttggac gcatccaccg ccccttcact
     2041 ggcgcccgcg cctacgatgg tgtgcagttc atcaataaat agaatgattt caccgttgga
     2101 atccgttact tccttaagga aggatttcaa gcgctcttca aattcgccgc gatatttggc
     2161 cccggcgagc atggatccga tgttgagcga aacaatgcgc ttgttgcgca tgctttccgg
     2221 cacgtcgccg ttgactatgc gccgcgccaa accttccgca atggcggttt tgccaacgcc
     2281 gggttccccg atcaggacgg gattgttttt cgtcctgcgg gaaagaatct gcaaaacacg
     2341 gcggatttcc gtatcgcggc cgataacagg gtcaatttta ccggcacggg cgcgggccgt
     2401 caggtccgtc ccatattttt ccagcgtctg gtatttgcct tccggattgt cgtccgtcac
     2461 gcgctggttg ccgcgcactt ccttcatggc ggccagataa ttatccttgg tcaggccaaa
     2521 ggtgtccgtc aactggtgca ctttgttttg cgtatccagc gcaccaagca tgaaatgttc
     2581 cacgctgagg tagtcatctt tgagcttttt gcgttcttct tccgcagcct gcattaccgt
     2641 agccaggtca cgccccatct ggggctgggt ggtggcaccg ctttgggtag gttcgtggga
     2701 aacttccctc tgggccgcct ggagaagcaa ccccgggtcg caagaggcct tgcggagaat
     2761 aggggaaaga attccgcctt cctgctccag caacgcaacc agaacgtcca gagaggagat
     2821 ttccggtttt ccaagccgtc ctgcagtttg ctgcgcctgc atcagcgctt cctgaaattt
     2881 tgttgttaga ttgttaagca tggtatgttg gtattttaga cataagaata actccaaccg
     2941 ttgaatggaa aaacaggatt tttcctgtta tctaaaaatt ttttgcccct ccggattccc
     3001 cgcaatatca cttgcacgct caacggatta ttggagagtc cgccctgaca aggaaatcct
     3061 gtcccgcttt cccatggggc tggcgcatcc ggtatcccgg caacagtgta aaattctgaa
     3121 cgccaaggaa aatatttccg gaaagaacgg cagccccgta ccggatagaa acgcaggaat
     3181 agcccttgcc catgctccaa aataatcgcg tgggcaaggg cggcaatgcc aaataccacg
     3241 tccgcggcct acccgactga cagaagacgg atttttccat tccattgatg gatacagcga
     3301 tattccccac ctgcaagaaa actaaaatgt gaaaaaatgc ttgctcgcat gataatggca
     3361 tggcagttac gtattcaata acggaaagat atggattttt tcgataggac aggtaaaata
     3421 gccattggaa gcaggctgcg catgctgacg gatgctgtga cgtctgatgc aagtcaaatt
     3481 tatgaattat acggcattga cataaagcca aagtggtttc ccctgctttt cgttttggcc
     3541 gacgggaaag agaaaacaat aacgggcata gcgaaggaaa tagggcacag ccatccatcc
     3601 gtgagcaata tcgttggaga aatgacggca agaggattgg tgaaaagcct tgaagaccag
     3661 aaggacaaga gacgcaccgt catcgccctg tctccacatg gaaaaagggt cgccgccatg
     3721 ctgacagagc tttgcaaaga cgtggaggtc gccgtagagg atatttccaa ggaaacgcgc
     3781 catgatttgt ggagagctat cggcgaatgg gaagaccggc tcgctgagaa gagcctgctg
     3841 caacgggtca aagaagcccg gaaaatgagg gaaagggagc atattagggt agttccttac
     3901 gaaccgtgtt acaaaaatac gtttaaatca cttaacgtac agtggattac aaaacattgg
     3961 aagatagaac cgcacgatct ggactatctt gatcatccgc aggaatatat tatcaacaaa
     4021 ggaggcttta tctttgtagc cctttacagg gggaagcccg tcggagtttg tgccctctgc
     4081 aaaatggatg acccggtata tgattatgaa ctggcgaaac ttgccgttca ccctcatgcg
     4141 cagggcaaag gcatcgggga aatactttgc catgcagcca tagacaaagc aaaggacatg
     4201 aaggcgaaaa ttctttttct tgaaagcaac accctgctca agcctgccat acataca
//
LOCUS       DAWEMX010000169         4175 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7630_length_4175_cov_8.177427, whole genome shotgun sequence.
ACCESSION   DAWEMX010000169 DAWEMX010000000
VERSION     DAWEMX010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7630_length_4175_cov_8.177427"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    929..938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1511..1520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          929 a         1372 c         1091 g          763 t
ORIGIN      
        1 gcgccgtcca gggcggagga aaggagctct ccggcgcgga ccggttcctt ccggtacagg
       61 cattccagcg ccagagcttc ataaaggcag gaaaaagggg ccttccgggc ggactccatc
      121 aaacgcgcgg aacggtcctt ccagcggtct ccgaactgca cggaggaagc ccaccacccc
      181 tggaaataac ggtcttcttc cgtcggctcc acgtccatat ccttcatgcg ggcgtatgga
      241 cggtacaggg ggtctccgaa caccaccccc tgccaggaca gcacaggcat gctcatccag
      301 gaagcctccg ccacggtata gccgtccagg aggcggtcca caaaaatatc aaagcggtgg
      361 caggcgccca ggaacggctc atacacgttt cccaccgtca cagcagcccc cttctccagc
      421 agggcggaac tccaccccct gccggggacc ttgaaatcgg cggcgctgaa ggaatgcaga
      481 tgcatggcta tcgccccggg ccggaaacgg aacgaaggat ccgaaaacgg tccgttcgca
      541 ttccccgcat accacccaca gtaaagagcc gtatcccggc tcaaggggaa tttttccggc
      601 agggtctggg gccagtcatc cagataagcg ggaatgcccg cctccctcac gcgctcgaac
      661 acgtcgtcca gccaccggtc cccctgggca taggggccgc cccggtccac aacggcccac
      721 ccccataaac cttttttctc cacgttcgcc ggttccgtca ccagccgcat gcaggtatcg
      781 ggcgtcagtc cgtccaggcg gcataccaga aacgaaggaa ggccggaacc cacaaaatcc
      841 tcccttttat taaaataggg attcacctgc cagtacttcc tttcataacc gcccaccgcc
      901 agcagagcca gttcggaatc caccgcagnn nnnnnnnnca ccgcagcgcg atccgtctgc
      961 atctggttga cgcctttccc gggaagggga gcgggcgttt catgccgtat cttcatcggc
     1021 aggtccgcca tcagcaccag cacaaaaatc ttccggttca tcaaggggga ggacgcaatg
     1081 ccggaaggca cccaccaccg ttgtttccgc gccgtctcca gcagcgggac gcggatcgta
     1141 tcctcatatt ccttccgcga aatctcgctg gtcatagggc aatccaggga aacgagattc
     1201 ccttccggaa cgccgcgcac ccgcgcatac tccctggcca tgcggcggct cagctcggat
     1261 tttccgttat aaaccacggc cacatgagcg ggcagcagct cataggcaca cgcggcggaa
     1321 aaccacgacg ccagcagaaa taacacggct aatccactct tgaacatgcc cctaccatac
     1381 ctcatcttcc ggggcaatca agcaggtgaa aggggcggcc acgaattcgc catttttccg
     1441 cttttcgctt cacatcctaa aaaaatacgc tttaataact cttgatgtat tgcaggcctc
     1501 ccccctgcat nnnnnnnnnn atcacacgca tatgattcag caaattgacc atatcggcat
     1561 cgccgtcaag agtcttgacg ccaccgtccc ctattaccgc gatgcgctcg ggctcggaga
     1621 gccccacatt gaggaagttc ccactcaaaa ggtccgcgtc gccatgtttg acgttgccgg
     1681 agtccacatc gaactgctcg aacccacctc tccggaaagc gctatcgcca aggctattga
     1741 gaaaaaaggg gaaggcatcc accacatcgc tttcaagacc aatgacatcg ccggaaatat
     1801 ctcccaggcc aaggaagcag gccttaccgt gctgaacgac ccgcccgtcc ccggcgctca
     1861 caacacgcag gtcaccttcc tgcatcccaa atgcaccttc ggcgtcctga ccgaattctg
     1921 ccagcacccc ggcggctgcg aatgcggcaa gtaacattct ccctattcac cataccacta
     1981 gaccaatggc tattgatccc aaattgattg acgatctgaa cagccgtcgc aagaaggtca
     2041 tcctcagcgg cggtcaggaa aagatcgaca agagacatga aaagggcgaa atgacagccc
     2101 gcgaccgcat ggggtacctc tttgaggaag gcaccttctc ggaaatcggc atgcatgtgc
     2161 gccacaactg ccacaacttc ggcatgggta agaaggaaat ccccggcgac ggcgtcgttt
     2221 ccggtttcgg cctggtggac ggccgcccgg tagcctgcgc ggcttccgat ttcctggccc
     2281 agggcggttc cctcggctac atgcacgcca tgaaaattgc ggatgctcag aagtatgctc
     2341 tgaaagccgg catcccgatg gtgaccgtga acgactccgg cggcgcgcgc ctccaggaag
     2401 gagtggcggc cctttccgga tacgcccagg tgttttacaa caacgtgctt gcctccggcg
     2461 tggttccgca aatctccatg atcctgggcc cctgcgcggg cggcgcggcc tactcccccg
     2521 ccctgacgga cttcatcatc atgcgcaatt ccggcaacgc gggcatgtac atcaccggtc
     2581 ccaaagtgat tgaacaggtc acgtatgaaa aatgcacgat ggacgacatc ggctccgccg
     2641 ccattcacgc caccgtgtcc ggcaatgtcc atttcgtagc ggacagcgac gcgcacgcca
     2701 tggacatcct gaagaggctt ctttccttcc ttccttccaa caacacggaa gaaccgcccc
     2761 ataaactgga cactccgctg gacctgagcg ctgacgaagg catgagcgac ctgattcccg
     2821 gcgataaccg cacgccgctg gacgtccagc ccatcatcag ccgtctggtg gacaacgggg
     2881 acttcctgga agtacacaag gacttcgcca aaaatgtcgt cgtcggattc ggccgcatct
     2941 gcggcgtggt ggtcggcatc atcgccaacc agcccaatgt gaaagccggt tgcctggata
     3001 tcgactcctc cgacaaggcc gcgcgcttca tccgtttctg caacgcgttc aatattccgt
     3061 tggtgaacct ggtggacgtg cccggcttcc tgcccggcaa gaaccaggaa cggggcggca
     3121 ttatccgcca cggcgccaaa cttatcttcg cctattccca ggccacggtg cccaaagtca
     3181 ccctgatcat gcggaaagcc tacggcggcg cctacatcgc catgtgctgc aaggacctgg
     3241 gcgctgacgc cgtcttcgcc tggcctggtg cggaaattgc cgttatgggt gcggaaggcg
     3301 ccgtcccggt actctacggc cgcgaactga aggctgtgga agacccggcg gaaaaagcca
     3361 agcgccaggg agaacttctg gaagaatacc gggaagcctt ttacaacccg tatgtggcgg
     3421 ccggcatggg acagatcacg gaagtcatca atccggaaga aacccgcgcc aaaatcgcct
     3481 tcgccctgcg caccctgctg aacaagaagg aagtgcgccc ggccaagaaa cacggcaaca
     3541 ttccgctcta acatccatcc caacggattc atatgttact tacaacactc gcttttgcct
     3601 tcgcacaggg catcgccaac gttatggaca atctgaacta tcaggttgtc ggcatcatgg
     3661 tggttatgct gtgcctggga ggcatggccg tcattctgac cgtcagcggc tccatcgccg
     3721 ccgccattca gaacagggcc aaagccaaaa ccgctgctcc cgtagccgtg cctgccgccg
     3781 ctcccgctgc ggcgggcaaa ccgggcatga ctccggaaat gctcgccatc ctttccgccg
     3841 ccgtggattc cgccatgacg gagcttactc cggaaatggt ggccgtcatc tccgccgccg
     3901 tggacgcagg cctggacggc atgagccacc gcatcgtgga aatcaagcaa tccccgggtt
     3961 ccggctatgc ggccgcgggc agggcggaga ttttcgcctc ccaccgcatc aggccttccc
     4021 gctgaactac tgcattattc acccttaccc cgcccaggcg gaatcattta acttaatatc
     4081 ccataccaca cactattatg aagaaacttc gcatcaccgt agacggcaaa gtatttgacg
     4141 tatccgtaga acttctggac caggtgtcct ccacc
//
LOCUS       DAWEMX010000170         4148 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7685_length_4148_cov_10.498656, whole genome shotgun sequence.
ACCESSION   DAWEMX010000170 DAWEMX010000000
VERSION     DAWEMX010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7685_length_4148_cov_10.498656"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3209..3218
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          918 a         1274 c         1027 g          919 t
ORIGIN      
        1 tttcttcctg taaaaaagca ggcggcggat atgcaatccg caaaaaaatg ttgtttatac
       61 ggatattgcg taaaataaaa aacttatcca ccaaagcgga cctgatctga tggggtattt
      121 tccagcttgc agtcctcaag ggtccatgtg cggggaggca gcaccttgtt atctttaatc
      181 agaatattgg cgcatcttct gaattggaaa ggtttctgcc cccatccctt aaactcgtca
      241 ttgtaaatga ccttattatt gatgaacttg aggttgtccg tggaaatggc gaacagcagc
      301 ggcacatcga acgtcttgaa aacattattt tctatcagca cattgcgatg atagtaatcc
      361 ctctgcctgt ccagctgctt gacttccggg taaatggaaa tgatggcatt cgtaaactgg
      421 taacgcgagg tcaggttgtt aatgaatgta tttttgcgga tcaccacttc atggcaggcg
      481 ccgctttcat accacccctg ggcatcccct gccaatagaa tggcggagcc ggaggaatgg
      541 tcaaacaaat tgccctccac cagcactctc tccggcgttg taaaaagaga tcctctggca
      601 cggttgttgc ggacgatatt gttgcggaaa accacggaag ggtaaaaatc cgcattctcc
      661 acggcatccc cggccctgac ggaggagggg agcggctctt ccaccgtaat caccatctcc
      721 gcggaattct ttttcactgc cgtcttcaca gtggccgttc cgccgggttc cagcgtagga
      781 ccgttgatga aacggatttt ttcaccggga agaaacacct caaaccccac cgcctgccga
      841 tgcatgtatt tgcacttcag ggtatgtctg tccactactt ccatcacgcc caggcaggtg
      901 gaatgaacat tgatggcatc atccatcatc ccttcgaaca gggctttctc caccacgatt
      961 ccgccccggg tattggaaaa atgggttgcg tccgctccgg cggtgtgtac gcgccctgtg
     1021 cctttcctga ccatgactcc gccgcctttc atatgaaagt cctccgatcg ctggaccagc
     1081 agggccatgc ctgagctctg gtgcagagat acgtcattca gtgatgtttt ccgagcccgg
     1141 tacaccacgc atcccggatg ggggcggtta taattccgaa gaaccagcgt atcccccggc
     1201 ttgattccct tctgcctgag gttccaggac agacggagac ggctcccgcc cagcggctcc
     1261 acatgcccgt tccagccgat gtccgatgta ttggcgataa tatgtcccgt ccctttctca
     1321 aaggccatgc agcttcccat tccttcttcc cagcctttcc cctggaaaac gaacctgttg
     1381 ttccggattt cgtaagggta ggcctttttg tctatttcca cttccgtgaa ccggtcatcc
     1441 gtcttcacga cgcgcgcctc cgtgcaccag gaccgttcgt aatccacgga taaacggttg
     1501 atgctgacat tttcgctgtc catcaccagg aggggaacca ccttgccgtg aaaaagaaac
     1561 agggaaccat tgccctccac gcggacattc cgcaaatcgg ccagaggcac acacaccgga
     1621 tgaatcagcg gctggtcatg gttggaaata tggaaactca tgttcaaagc cccctccgga
     1681 taaaaatggt aaatgcctct ggggatgttc aaaacgcccc cgccctgcct ccggagagcg
     1741 gaaacggcag cccgcagcgc cggcccctga tcttttctga cgccgggaac catgccgaat
     1801 tcagaggcat tcaccacagc tcccttcatc ccctgcccac ttcctgccgt cttccaggcc
     1861 agatctaccc agtccgcatg gtcgtaggaa ttattgtcca cccggtcggc catcaggtaa
     1921 agatgccgta tgccgttttc cgccaccgga atggaaaact tcttcgcggc catccccctc
     1981 ctcatcacgc cggaactcca taaaacctcg gacccgctca tcacgcggaa ttccacgctt
     2041 ccgtctccat ccgcaccgtc atcaattccg catgcacctt ccagcctcaa aactttcggg
     2101 ttttccggaa cgggcagggg aatcatggac gtagcgtgcg taccgattcc cgtggcatat
     2161 tttttgccgc ctatgctcaa ccccgcatta ctgaaagacc ggtccaaccg ggtttccccc
     2221 gtctcctggc gggccatcag cagactggag gcaggaactt ttacggctat ggttccggat
     2281 tgggcagctt cttcctcgcc ccgggactgt gcctggaata atcccgccaa acacgctgca
     2341 acgacaaaac tccatgaaaa catccggctg aacatatttt ttatactccg ttcccgtatt
     2401 ccttctatca gaaaaaatat ggatcgtcca gaagcttcca tcagagaaaa agagagattg
     2461 ccttccatac cgaattcagg gacactgtct ccatgcgatc agcattcttc ctactgcccg
     2521 gcatcctgcc ggctctttcc gcctgcgggc gcggaggcgc cacgcttgaa acggataacg
     2581 tccaggcgga atggttcccc aacggcagca gaacggcgat tgtcctggaa ggggaaagcg
     2641 aacccgtttc cgaacgggaa atccggcatc cccattccgg catgaccctt ttttaccatt
     2701 cgctggggga cggcagagga actttggaaa tcgcaggagg ggttccggaa acggctacag
     2761 taacatgcag ccagggagcg gcttccgcgg agctgcattt caaaacggcc tctcgggaaa
     2821 tacttctgaa tgacgtacgc cgcccggacg cgaacgaaaa aacggccttc ctgaatggct
     2881 ggagctgcca ggaacggggc acagccctgc tccgttccgg gacagccgga gccatgacca
     2941 cggaaaaaaa cgtcccatga atcttctgag gatactgaaa acgggaacat ggaaaatccc
     3001 gtgcgcactt ctgcttgccg ggggtctggc tctggcatgc gtccagtgtt ccgtccgttc
     3061 cctggtgtac ctgccgcccc agcccaagga caaaaccgcg tatctggaaa aacgtgccgc
     3121 ctgggaaccg catcggcgca ccttccacca taatggggcc aaactcagcg gatggcttat
     3181 tgaaaaaaag gggcagcccc tcctggtann nnnnnnnnat tacggaggga acgccatgga
     3241 tatttccatg atgcttccct acctggatcg ttttccccat gccaagctcc tggtcaatta
     3301 ccgcgggtac gggctcagca ccggctctcc tacggaacag gatatcatgg gagactccct
     3361 ggctatcctt gactccgtgc tgaaggaaac cggaagaacg ccggacgacg tcatcctggt
     3421 cgggcaaagc ctcggttccg gagtcgctac ccaggtagcc tccgtcagac acgtgaaaaa
     3481 actggtgctg ctggtccctt ttgacagcct tctggaaact gcccgcggtc tcttcccata
     3541 cctgcccgtc aggcttctcc ttccggacca cttccggtca gacctggccg cgcccaaagt
     3601 cgcctgccct gtcagcatcc tggctgcggg agcggatgaa gtcattcctc cgcaccacgc
     3661 caaacggctc cgggactgtt tctccgtacc ggtcagttac caggaattcc ccggagccat
     3721 gcataatacc atctggctca gccccggctt tgataaagcg ttttctcaaa gcatcgggta
     3781 ctgatctccg ttttactccg caggagccag agacacgcgg cgcaggcagc ttccttcatg
     3841 ttccagcagc agccatgagg tttcttccgg cagcgcttcc ggaaacaggt tggcccattc
     3901 gacaatgagc acagtctctc cgtccagata ctcttcccac cctatgccgg tcagctcgga
     3961 ctcatccttc agccggtaaa aatcaaaatg gcatgccggc aggcgtccgt cagcatgctc
     4021 atgcaccagg gaaaatgtcg ggctggcagc cgcctcggaa ctccccagcc cctccataat
     4081 gccctgggtc tggggcgtct ttccggcccc cagctcaccc accacgccca gaatttcgcc
     4141 gggcatta
//
LOCUS       DAWEMX010000171         4139 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7698_length_4139_cov_7.592556, whole genome shotgun sequence.
ACCESSION   DAWEMX010000171 DAWEMX010000000
VERSION     DAWEMX010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7698_length_4139_cov_7.592556"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    679..688
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3885..3894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          972 a         1263 c         1032 g          852 t
ORIGIN      
        1 tgccgggaat cctgtccgga ttgccgacct ccttcatggt gccgttatga aaggcggccc
       61 ccgtgccgtc cgcgtgcatg aacccgttcc agatcacacg gccgaacatg cggtttttgg
      121 gcagcatcct gtacttgtac aagcccatga tctgctgctc catgccgata atcctgtcgt
      181 acagggcaat aagctcctcc cccttgtccg gacagaattg tttaagttcc tccaccggat
      241 acaccagatg cacgcggctg cccacgatgt ccacaacttc cgtgggggag ttttccagca
      301 tcttcttcca gtcgctgttt ttgctgactc ctcccacgaa aactccgttc accttgccgg
      361 acaaaatgga taaatgcacc ttgggagcct tcttataatt gggggtgaaa tactcaatgt
      421 agccaagccc cttgttcttc gccctgatga tgttgacccc ctccttcagc ggataggagc
      481 tgtggcctcc gtcccgcccg aagttgtgga taaccagatt cagcttttca cctttgggat
      541 cccccatgac cagcaacacg tcctccccct cctcaaaaaa gatgccggtg ggattttcaa
      601 atttgctgta ctggctggta cgcaggcgtt ccgccagatc cttgaccggt tcatacggtt
      661 ccgccgtaag ggcgcgggnn nnnnnnnngt ccttgattct ggcgatgcgg tcgcgcgtga
      721 cattgggctt caacgccagc atgtattcat tggcgaacac attggaatcc aatgctttca
      781 aaccctcctc cgtaatcggc gcaccccctg cttcctgggc ggaaagcacg ccgccgccca
      841 gcacgccgca gagggccagt aaaagaaccc cctttttcaa caatggtgag aaaatcatat
      901 ttgttaaata atactgttca gtttccggaa ttgtttcaag gaaaaacgcc cggcccctcc
      961 catgcccggg aaaaagcccc tttgtgtgac atggaacgct caggcccagg gaaaaaaact
     1021 gtccatcccc cttgacgggg gccgtataat gacgctcgca aaacattttt ccatatgaat
     1081 acagaaacac atcaatttca ggcggaagtc cggcagctgc tggacattgt catcaacgcc
     1141 ctttacagcg accgtgaaat ctttgtccgc gaacttgtct ccaacgcttc cgacgccctg
     1201 gaaaaactgc gcctgaagca gctgacggac tccaatattt accagccgga caagcccctg
     1261 gaaatcaccg tagccacgga taaggaaaac aaaaccatca ccatcgcgga taccggcatc
     1321 gggatgacgg aagcggacct ggtggaaaat ctgggaacca tcgcccactc cggcaccaaa
     1381 aaattcatgg aagtccttaa gcagaagcag gaaggcggag cggacctgat cggccagttc
     1441 ggcgtgggct tctacagctc tttcatggtg gcggaccgcg tggaagtgtt cacccgctcc
     1501 tatgaaccgg aagccgcctc cctgcgctgg tcttccgacg gacgggaagg ctacagcatc
     1561 gaaacgctgg cggaaccgct ggaccggggc acccgcatcg tcatccgcct gaaagacgaa
     1621 tatgaagaat tctcccagga ataccgcgtc aaggaactcc tgcgccgtta ctccaacttc
     1681 gtggggttcc ccctcaactt caacggagaa cacatcaaca ccgtccaggc catctggtcc
     1741 aagtccaaat ccgacgtgaa gccggaggaa tatgacgagt tttaccagtt catctcccat
     1801 acggatgaaa agcccctgtc ctacatgcac ttcagcgcgg acgcccctat tgccctgaat
     1861 gccctgcttt tcatccccag gcgcaatccg gaaatgttcg ggttcggccg cgtggatgcc
     1921 aacgtgtccc tgtactgcaa gcgcgtgctg attgacgcca agccggaagg cctgctgccg
     1981 gaatggctcc gcttcctgaa cggcgtggtg gacagcgaag acctgcccct gaacatttcc
     2041 cgcgaaatgc ttcaggacaa ttccctggtg cgcaaaatca gcgacatcat caccaggcgc
     2101 ttcatcaagc atctggaaaa actggcgaag gacgataagg aaacctacag ggaattctac
     2161 gcgcaattct cccgctacct gaaagaaggc gtcgtcacct cctggccgaa caaggaatcc
     2221 ctgggcaagc tgctccgttt tgaatccacg tccacggaac cgggggaaac gacctcattt
     2281 gaggaatacc ttacccgcat gaaggaaggg cagacggcca tttacgcgct taccggccct
     2341 tcccgctccc atctggaaaa cagcccgtac ctggaagcct tcaaggcccg cggctatgaa
     2401 gtggccttct tcacggacca cggggacgaa ttcgtgctgg actccctgtc cagcgtggac
     2461 ggcaagcccg tcacgatgat cgaccgcgcc gacgtggaac tccccgccct ggaagaggaa
     2521 cagaaagacg ccctgcccca ggaggaagcc gcggccctgg aagaatggct gaaaggactg
     2581 tacccggaca aattctccaa agtcaccctg ggcaagcgcc tggtcagcgg agccgccgta
     2641 gccctgcaaa gcggcaatga catggggccg gaaatgaggg cgtacatgaa agccatgggt
     2701 caggaagtgc cggaaagcca cccgcagctg gaactgaacc cctccaaccc tctggtgaaa
     2761 aagctttccg ccctgcggac ggaaaacccg gaactggcgc aaatggtggc ggaccagatc
     2821 gcgaataccg ccctgctccg cgccggcatg ctggacgatc ccgccgtgct ggcccagtcc
     2881 tcccaggccc tgatggaaca gctcctgctg aagtaggggc ccggaagaaa cagcttcctt
     2941 ccccaacgcc gctcccagcc ctccccgccg ggggcggctt tttcatttct cttcccgcca
     3001 tgaacgcgtt acgccacatg ccccgccatg ttctattcct gaggctggcc tatcaagcgg
     3061 gcatgtgcct gctggccctg gtcgccgtaa cgctgaccgc cattgacctg acaacggaag
     3121 cggcggaatg ggaagtcacg gcggaccgca ccatctactg gatttttgtg ctggattatg
     3181 tgatccgctt ctccatcagc cggtccaaat ggctcttcgt taaggaacac ccctgggatt
     3241 tgctggccat tattcccttt gacgccctgt tccgcctgtt cagattcgcc tcgctgggag
     3301 aaatcttaag gctcgccaga tatctggacc tgttttccta tgccatgagg ttcaccacgc
     3361 gcatccggcg tttcttcaac acgaacggat tcaagtatat ctgcatcgcc gccctggtca
     3421 tcatcctgct gggggccgta ggcatccatc tggcggaagg catgagcctg cccaacggca
     3481 tctggtggtc cttcgtcacg gctaccacgg taggctacgg ggatacctat ccggtcacca
     3541 cttcaggcaa attcctggct gtattcctga tgctcacggg cattggattc gtgggaacgc
     3601 tcaccagcac catcacttcc ttctttctga gtccccacgg cggagaagcg ctgccgtacc
     3661 gggaggagga aattgaggcc atcaaaaaga aactggacga cctttcctcc atgacggatg
     3721 aagacataga caccatggcg gccctgctga agaccctgcg ggacgcatcc tcggcacctc
     3781 cagcggaaaa agaggaaacg cctgatatcc attccggaaa aaactgaaaa gatccgtttg
     3841 gtattacatc ggaaaagacg tcctgtccgt accagggcaa acacnnnnnn nnnncgtttt
     3901 tcagtgagaa gctcttaagt ctctatgttg attaccaact gcttggccac ctttaaaaaa
     3961 aagaaacgcc gcaataacaa tgttggccaa aggaaccagt tttaaaaaca cccaccatcc
     4021 gctctctcct atatcatgaa gccttcggac atccgcaaca atattgaaat atataaaaca
     4081 aataaaaata ataactgtca acaccaacat tcctgcatca ttccctgttt tactccaca
//
LOCUS       DAWEMX010000172         4137 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7701_length_4137_cov_8.311367, whole genome shotgun sequence.
ACCESSION   DAWEMX010000172 DAWEMX010000000
VERSION     DAWEMX010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_4137_cov_8.311367"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2822..2831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          912 a          945 c         1232 g         1038 t
ORIGIN      
        1 gttcgtatcc cgatgctcgg agggtgtatg tagggaaggc agaatttagt ttgtcgcgcc
       61 ggtcgcccaa aggagttcct cggtgaataa tccaagctgt tggaggacat agcctattgt
      121 tcccgttctt ccaataaccc acccaccaga gagccagtta tctattgttc agccatttgt
      181 tccctgatta tctctgtttc ttgatacatt tttttattat ttttgagaaa ggacaggtta
      241 gttgaaatag gtgaattaag ggttcaatgg cctgaaacct aataacgacg ggggagaatt
      301 gggacgatgg gcgtagcttt ctccttatca ttcgaaaatg aagttatgaa ttatcgtgga
      361 agaaatctcc attacctgtg aagaagccaa ggaagtggaa atcggttttg aaggaaagga
      421 accggttatt gcaaacgaag catggaattg catcactacg cctatcattc ccagaatttt
      481 tgtgacggcg tgtaaaggat gaagcagccg atgtctggaa gctccgtatt gagggagcgg
      541 aaaggcgatc cggcaatgta ggcggtaatt tatcagtccc attgtcttaa atgggattcc
      601 aaatttattg gggtatcaaa aataccagta ttttcaagtt ttgatcaagg aggggaggtt
      661 tgtggacttt gaggcggaat tatgggatga caacaggttt gaggaaaact gttgaacccg
      721 tccacggtgt gccgggagga tttcttcgga caggaaaagg ccggggctat ttgagccatg
      781 ccaggaattc atggttgccg tcagttcccg tgatagggga aggttcgcac cccatccagg
      841 aacggttcag ttcgtccgtg acgaaagtgc gtatggagtc caccgccctc cggtgcaaag
      901 ccgggtcccg gacgatgccg ccggggccta tatcttccgg ctggagttca aactggggct
      961 tgatgagcac cagggcatcc cccccttttt taagcagagg aaaggcggcg ggaaggattt
     1021 ttttcagaga gataaaggag acgtcggaaa cgatcagatc cacttgttcc ccaatatcct
     1081 ccggagtcat gtagcgggcg ttgaattttt ccttcacaat gacgcgggga tcctgccgca
     1141 gtttccagac aagctggttg gttcctacgt ctatggcatg caccctggcg gctccgtgct
     1201 ggagcaggca gtccgtaaaa cctccggtgg aagccccaat gtcaaggcag accttgcctt
     1261 ccgcagaaac ggggaaggca ttcagggctc cttccatttt caggccgccg cggctgacat
     1321 agcggggacg gtttctaatg gtgatatccg cgtcttcatt aaccttgaga ccgggcttgg
     1381 tagagggatg cccccccacg ctcacttctc cggcaatgat gagccgctgg gccagctccc
     1441 gggattctac gagattgcgg gaaaccagaa ggatatcgag tcgttgcttg ctcatgattc
     1501 ggaaaaaggt gtgaacgcag gtatttcatg aaaccgcatg aagaatcaag aaaaaccgag
     1561 cgcagagagg cattcgggcg taaaaatgcg gcattcctcg tactggagca ttttacgatt
     1621 gattcaaggc cggtttatag gagactctgc ggcggatgga acaccgctac aggcaattga
     1681 tgcgcagagt caggctttac ctgctgacgg aggtgcgcaa aaaatcgtgg gcaagcaagt
     1741 tcctttccgc cagggttttt gattctgttt actggtcatg ggacaggcaa tccgttgcaa
     1801 cgggagctgc ctggggggct gccgcagcga ttgcccctct tcccatgcag agcctgtggg
     1861 gcgttttcgc ctgcctgtgg cgcaagggta atattcccgt agccattctg atggcgtggc
     1921 tctcccctcc cggctttacc ttttttgcca ttcccgctca atggtggctg gggtggttcc
     1981 ttttttcgat tttggggttc cctacgtccg gcgcgaactg ggagatgctg aaagcggggg
     2041 ttcagcagtg gtcctggacg ccgtttggcg gattgagcat cggcatggta agcctggaat
     2101 tcgcagcggg ctggatggtt tccagcgttg ttctgggcag cctgtgttac gggctggtgc
     2161 agttgggatg gaggatgggc cagttcctgc acagcagaaa aaagagcggc aagttgtaat
     2221 agcaaaactg ccgtctgtgt aaggcagggc ggaaccggaa agccgggcag tccggaaaaa
     2281 tcagacggct acttccatca gcaaaacggt tgagttctgg agggcttcta tggagacggt
     2341 ttccgtatcc gtaattccgg caccgtcccg actgtgcaat atggcatctc ctatttttat
     2401 ttctccgtct atcacgaaga tgtatacgcc cgtgttccgt cccttgaatt ggtattcccg
     2461 ttctatgccc ctgtccagct ccgcccagga aaaccaggcc tgctgcatga tggaaatatc
     2521 cgtttccgga gctaggaagg tagagatttc attatgcttc aacagggggg tgatgtcgta
     2581 atcccggtac ctggggggcg tattgatcct gtccggaatg atccatattt gcaggaattc
     2641 caatggttcc gtgaggctgt cgttgaattc gctgtgctga atacccgttc cggcgctcat
     2701 cacctggatt tctccgcgcg tgatagtgtg gctgttgttc aggctgtctc cgtggcggag
     2761 ccttcctttc agaggaatgg aaaccacctc catatttttg tgcggatggg taccgaagcc
     2821 tnnnnnnnnn naaccttgtc gtcattgagc acgcgcaaag ctccgaattg catgcgttcc
     2881 gggttgaaat aattggcgaa gctgaatgtg tggtgtgtat ccagccatcc gtggttggca
     2941 tgtccacggg aggatgcacg gtgaatggta atgttcatga ttgatatagg gggagtggga
     3001 gcggttttcc gcttgcagaa tgtttgaccg gctgcaactc tgggctgttc aaggcgaatg
     3061 acattatttc caggaccgta aaataaatcg gcaacacaaa ttggtggtgc aatgtctctt
     3121 cttttagggt agcgtgctgc agtacccgtg caaagaagac aaggcttatg gctcagcgga
     3181 catttatgag atggatcaag ggactgattc ccacagtgat tattttgctg ggggcgttgt
     3241 tgctgggctg catatgctac atctccatct ggggggtgcc ttccttcgtt gtgcagcggg
     3301 tggagcaggc tcttctggaa aagggcattc cctcccatat agagacgctg aaagttactt
     3361 tgtggccccg tgccgtcgtg acactgaaaa atgtggaatt gctggatccg gaggccatgc
     3421 cggaaactcg ccggcccata gttctgctgc atgaggcgga tgtggcgctg aattggaaaa
     3481 aactgataga aggacaagta gtgccggagc gggtgaatgt gaaggggatg gacgtctccg
     3541 tgcctgtaga tgcggggaat ccggaaaaag ttttttccgt aacgggggtg aacgcggaat
     3601 tgaatctggg gcggcccggc atggtggaca tcgtcaaggc agatgccgtt gtgcagggca
     3661 tccgggtgac ggcccagggg gcattttcca ttggtcagga tgattccgga gactttacgc
     3721 tgacggctca ggatatgggc gccgtcagag agcagttgaa tcaggttctg aattacatgg
     3781 accgggtgaa gtggcctgat gcatctcctc cccgcctgat tgtcaattta agcgacgatc
     3841 cgaaaggcgg cgtgcgtgta ggcatggatt tgcaggcgcc ttttctcagg tacggaaaaa
     3901 tcatggtcag ggatttcctg ttcagcgggg attacgcgga ttccgttatc atggccaaac
     3961 actttaccat gcgggatgcg gaaacggccg gtttcgtcag cctttccctc caggccgatt
     4021 tgaaaaagcg cacgctgatc tgggatgtcc gcagtacggc tcctctggtc tcctgggcgg
     4081 tggctattgt tgatgaagct ctggtgccga aggaaatgaa gtttctgagc gagccgc
//
LOCUS       DAWEMX010000173         4055 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7853_length_4055_cov_8.962250, whole genome shotgun sequence.
ACCESSION   DAWEMX010000173 DAWEMX010000000
VERSION     DAWEMX010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_4055_cov_8.962250"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    270..279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1852..1861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3509..3518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          892 a         1004 c         1210 g          919 t
ORIGIN      
        1 tgaattccgt gtacacggcc tgggccgccc cgcgcgcgcc atgggaaacg tggtgggacc
       61 gtctgaggga ttacgtgctg cctcgccgcc tgaatcggga aggggaggtt tccctaccca
      121 acagggacgc catggaccgc atgacggata ctacggctgt ggaggcatgc cagaagctgg
      181 ccagcggcca tatgtcctat attacgccca gccatgacgt atggttcaag tggtcggctc
      241 cggatgaccg gggaggcgac gaggctgagn nnnnnnnnng aaagtgccct gagagaattg
      301 tccgtttcca atttttatac ggagattcat gagtgttttc tggaccgggt ggctttgggg
      361 accggaagtc tgtttacggg cacatcctcg gacggaaggc tgttgtttac caatattccg
      421 tgcggacagt tcgcctgtgc ggagaatgcg gaaggccggg tggataccta cgtcagggag
      481 tttacctaca cggctcatca ggcacgctcc atgttcgggg tgaaagctct ggggcccaag
      541 gctcgggaag ttctggagcg cggaggcaat ccgtatgcca caactctgag gtttctgcat
      601 gtggtgcgcc cgcgcacccg gcgcagccgc cgcagggagc aggcctccca catgccgttt
      661 gaaagcgttt acctgtctct ggacgaccag gtgattgtgg aggaaggggg atacatggag
      721 ttcccttatc tggtaacccg ctttttgaag tggggcagcg gcccgtacgg tctggctccc
      781 ggcaggctgg tgtttcccgc catccagcag gtgcagttcc tgaaccgtat tctggacact
      841 cttggcgagg tggccgcctt tccccgtatt ctggagctcg ccaaccagat tggggaggtg
      901 gatttgcggg ccggcggcag gaccgtcatt accccggagg ccgcttccct tcaccttccc
      961 cgggaatggg ctacgcaggg caggtatgat gttgggatgg accgtctggc acagaagcag
     1021 gatgcgatac gccgtgccta ttatctgcct atgctggagc tttggagcgg gcaccgcggc
     1081 aatatgactg ccacggaggt catggcgcgg gagaatgagc gcgtcttgat gttttctcct
     1141 tccttcacgc tgtttgtgag tgatctgtat tccacaatga cacgcatttt ttccctgctg
     1201 ttccggatgg gcaagtttcc caggccaccc cgtgcggtat tgagggtagg gagggacggc
     1261 tccgttgccg tgggagaacc cagggttgtc tatcagtcaa agattgccct ggtgctcagg
     1321 cgcttgcaga gtgaagggat ggaccgcagc cttcagcggc tgaatatgat gatgcaggct
     1381 gccccggatt tggcggatca tgtggactgg gaccactgtt tccgcctgtc cgcccgtgtg
     1441 gacggcgccc cggagagcat gctgaggccc tgggccgatg tccgtgccat gcggaaggaa
     1501 cgggcggacc tccagcaggg agcctccctg gctccggcgg aagaggatcc ttatgcttcc
     1561 ctcaatccct tgcttgacca gttaaccgcg attcagaaat gaaccaggat acgacattgc
     1621 agcaggaggc ctccgtccgg gaggcccgtt tcaaaaggcg ccagcttctc cgggtgtttg
     1681 acacccagga cgggcgtgag actctttcct ttctggaagc ccgtttccag actgatttgc
     1741 ccgtttttca aggaagtccg gggaattacg accctctgga cgccatgagg agggatgcct
     1801 acagggagat ctttctgtac atccgccgcc agctccaatt agccattaaa gnnnnnnnnn
     1861 naagggatgg agaaatctct ttccaagttt aagacgccgg aggctctggc gaagagttac
     1921 gcggagctgg aacgcctgcg acgttatccc ggtgtggaga atgaagaaca gatggcgcgg
     1981 ttccgccggc tggccggatt gccggagtcg gaggaagaat accgtctgga acgtcctgaa
     2041 tccacgccgg aaagcgaatg gaatgccggg ctggcagagc gtatggcccg cacggcctat
     2101 cgttacggag taccgccgga ggcaatgaat gctcttcagg agacaatggc ccaggcatat
     2161 gacgaggccc gtgagcacat ggaagatgcc cggatggaga tggaaatgca ggcggaacaa
     2221 tcccttcagc aggaatgggg cgccaattat gaacgcaaca tgggcagggc tgccgccgtt
     2281 cttcggcggc ttgcctctga aacaggggtg gatgcggacg ctttactgga taatccggga
     2341 ctcggttcca atccggatgc aatccgcctg ttgtaccagg catcacgcct gcttgatgaa
     2401 gctcctctcc atcatacagg aaatgcggcg ccttcccctg cggaggaagc catgcggatg
     2461 gaatcggatc catcccatcc tctttatgag gcgtatatga atgtgaacca tcccaatcat
     2521 aaatatgcca atgagttata tgatcgttta actacccgtt aatttcccgg acgttttaac
     2581 cggcggagtc ttataaaaag caagcccggc ggaatgcttt ccgccgggct tttaatagtc
     2641 attgtttcat cagggaagga gggggtaagg gctggggcct taagctgccc tcctaccatt
     2701 agcgtcccgg gaaggggctt gtgttcaaaa aaagttggaa aaacattttt ccctgtatgg
     2761 gagactagtg aggttaaatg ctcatcatat ctttcactgt aagtgagtaa agagggagat
     2821 atccggtatt ttttcttatc agcgcaaact tctgcattaa ccgtctcttt atgccggcat
     2881 ttttcaattg agcctgttac gggggaaggc tgatgctatg tctattgact ggagggaggc
     2941 aatgaaaaac cccgtccgga aaggaacggg gttttaagaa aaatggtacg cggtatagga
     3001 tttgaaccta cgacctccac catgtcaagg tgttgctcta ccactgagct aaccgcgcat
     3061 ttgtaagtgc ggacggatta ttacctgatt aaggaggaaa gagcaatact ttttttgaga
     3121 acgcctcatt ttggggacgg gggacggaag cgttccttcg gggaaaaaga cactgatttc
     3181 tcttcctata caaagtattt ctgctagtct gagaggaacg tttcacagag tatgatgaac
     3241 agacatttta tttttgcatt gtgtactgtg ctggggttca gcgtttccgg catttccatg
     3301 gctcaaagcg ttcccacgga caaggaagcc gcagccaagg ctgccaaggc ggcgaaagtg
     3361 aaattccgct ggggcaagag cctgaaaagt gctcaaaagc aggcggcgga aaccggcctt
     3421 cccattgttc ttcttttcac gggaacgtcc tggtgcggct tttgtgttaa gctggagaaa
     3481 gaaatccttt ccaaaaagga cttcaaacnn nnnnnnnncg tgccggccat ggtggtggtg
     3541 gatgcggagg gcaaggagct gggccgcacg ggttatgttc ccggccgtac tcccgcccag
     3601 tacgttgaat ttttcaagaa atacgcaccc aaggctgcgg aaggcaagta agtcaggtgt
     3661 tccggagagg agcttgatta acaggagcgg cggaagaaaa tccggcgctc ttttttgtgc
     3721 cggcaagaga ggctggaaat ttgttcgctg gactgcttta aggccaccat cgtataaaag
     3781 ttatgacaaa gcatattgaa tgaatgggtt acgtatcagg tcccatctgc atgcctgaat
     3841 ttacgaatgc atttagcggc ctaaaggagg atcggaaact cacccatgac gaattggtga
     3901 gagccatccg gttcatgatc gcagcggaat atgaatccat ccagctttat acccagcttg
     3961 ccgcctccat tgatgacaag ctggctcaag aggtgctttg ggatatttcc aatgaggaaa
     4021 aggaacacgc gggcgaattt ctccgcctcc tccag
//
LOCUS       DAWEMX010000174         4046 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7876_length_4046_cov_10.315209, whole genome shotgun sequence.
ACCESSION   DAWEMX010000174 DAWEMX010000000
VERSION     DAWEMX010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7876_length_4046_cov_10.315209"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          949 a          919 c         1184 g          994 t
ORIGIN      
        1 cgaagggaac catctacgtg aacgggaacg cggacatccg tgacgggtcc agcgcactga
       61 acggaagcgt cggctaccgc tacgacttct gatattttct tgttttctcc gcaggtaaat
      121 agatattccc ggggccgcat ctgataatct gcatgtagcc ccgggaagtt aaacggtctt
      181 ttttcaaaat taatgaaaga tgggaattta atagcaaaaa gaaaaagatc tcgtgttgaa
      241 atcgatttac aaaaagtaaa gtgaagatta gaataaggaa aaatggcgaa aagggggggg
      301 agagacatgg ttccttcttt gctgaatttt aatcggacaa cgaataatat gaagacgtgt
      361 atgatgaagt ttctcctgct ctgtatggta gtaatatcca gtttcttttc tgttcctgct
      421 cgcgcgagtg aatattgggc taaaaatgtg agcagggaat cgggatggaa tgatgtgccg
      481 caggaaggag gggagtgctg ggcaatggtt gcgtccaata tcctctgttg gtggcaggat
      541 gagctggaaa aaacatatct tttgccggaa gacgccccaa gaaaatttga agatatcaag
      601 ctttctcttg tgaatcattt taatagggga gatcaaggtt ggtttgtagc gagagctttt
      661 gcatggtatt tggaaacgta ctattcctct acagttaaac tcccgtttcc gaaaaacaac
      721 tggtttgtgg aaaattgtac aaacaagaac gcttcttata catcgcaacg attgaaagag
      781 ctcttgcaag cgggggtgcc tgtcagtgtt gccaacgccg cccatgctgt aaccgtatgg
      841 ggggtggaat acaatgacca gtcaaacagt ctttccaaga tatggcttac gaattccatg
      901 cccaacgcgg gatgggacgg cttggaggaa tggaatgttc agattgtcac ttctgagaaa
      961 gacgggacag aaggtttgat gttgacattg gcaagtaacc ctggggaaca acagacttgg
     1021 tccgggaata gttttgacta tctgcttttt cagccgggag atattacgtt gccattggtt
     1081 ccggaaccat ccgtttcctt tctgatgctg gcgggaatgt tcgggtagac actaagaagg
     1141 agagtgaagg agtgaactgc ctttccttct gtttttgttc cggttgtagc gacggagggt
     1201 catttttcaa tggcagagtc ggatgaggcg ggcgttcgga tggaaggagt tggggtggaa
     1261 ggttttgcgt attgtgaagg aggattccgt tttggaactg taggtgaaac cggataaagc
     1321 ggaatgctac atgcttgcca agcgctatcc gattgccgca tggtccggaa aactgggccc
     1381 caaggagaag gaggggggac aggcaaatac cggaaggttt ttatgaagtg gagccgtccg
     1441 ggctcaatcc ccggagcaat gatcatctgt ctttcaactc gggtgtccga atgcctatga
     1501 ctgctccctg aaccggacgg gaagctccat catggtgcat ggacgggatg tttccatcgg
     1561 ctgcctggcg atgaccgatt ccggaattga ggagatttat accatggtgg aacaggccct
     1621 ggttcatggg caaaaaaacg ttcccgtgca gatttatccc ttcgtgccca ctccggcgcg
     1681 tttgtcagag gaaaaggatt ctccccacgc cgccttttgg acggacatgg accgaacgtg
     1741 ggactggacg gaacggacct gtactccggc ccgggtgaag gccgtccatg gcaggctggt
     1801 cgttgtggag caataaaaat cctccggttg aggaccggag gataaaggaa ggcctgcgga
     1861 acaggggcgg caggagcgcc cctgataccg tgtgtccgga atgctccgga ttagaccagc
     1921 tgggcaatca attcttcgcg gtcgcccggc agaaaatcca tcggggcaat ttccggcagc
     1981 gtatagcagc ctgcggccag tttcatgctg gcgcgggcgg aagccaccat gacctgggcg
     2041 gtcagggccg gattgttgat gcgcatttca aacgtgaaca tctggttctg cgtggagccg
     2101 gaaacgccct tgcgttccat cagcacaccg tggcccatgt ctttcagcac gtcgatgtcg
     2161 ggcacctgca gcacgcgggt gtcgtcatgg ctgaagtagg aatcggattt aatggcgaat
     2221 tctacgtcgg agaatttggc gccttctttc agcaccacat aaaccatgcg gcggtgtacg
     2281 ccggaacctg tggggatggt gagggaaaga gcgtccgcca ctccttcctt ggaacgcgcc
     2341 acgacgctgt ggcccatgct catgccgggg ccgaagttgg tgtacgtaat gcctttggga
     2401 gccatggcca gcatcagggt acggatgacg gagtccgttc cggggtccca gccggcggaa
     2461 atgatggaga cggcgtcatg cttgattgcc tgggctccca gggaacggcg cagggccacg
     2521 atgtccccgt gaatatcgaa gctgtcaact gtgttgatgc cgcgggcgag cagaggcagg
     2581 gccgtttctt ccacgctgcg ggtgggggtg cagagcaggg ccgcgtccac atggcccagt
     2641 tcctccacgt cgcttacggt cttgatgccg tgtacagctt cgctgccggg gcggcgcacg
     2701 atacctgcca gttccatatc gggagcggcg cgcaaggcgt ccacagcgta tttcccgatg
     2761 ttgccgtatc ctacgatggc tactttaatc atcttggtag taaatagtaa tggttcctaa
     2821 tggggcggaa tgtccacccg gcattttttt cctttggcgc atgtttttta agtgttttgg
     2881 gaacgcatgg caagaacgga gaatgacagg gggcttcgtg gcatgattgc cggttctttg
     2941 aacggggggc ggaggaatag agtctgatta ctcggaactc ttccaataaa acgatgaaaa
     3001 gcaaattgat tattctgagt atcgccaccc ttctggggtg cgtttcctgc aatacgatag
     3061 gcggggtggg caaggatgtg gaagcggtcg gcagcgacat taattccgcc gcccgttcca
     3121 ccagccgggc catgtaaaaa ggtcatgtag agaccttgga cgaaagccgc ttcatccacc
     3181 cggatgaagc ggctttccgg taaaaaatgt gctgccgccc gcatgtcctg atttacagat
     3241 ggccgcaaag gtttggcgtg ttccatgcag gatggagccg gaccttgcca attcgttggg
     3301 aatcccatga ggatgactgc cattcagggc agaatggcag gaagcaaagt actgcctctt
     3361 ttccattaaa agagggctca aagcagcgtc cggcacttcc cgggccttat tgcatggact
     3421 tgctggtggc ctgggaggcg ctgtcaatct tgcttcccat ggcttccacg tcttttccta
     3481 ttccgctgat cgtattgcag gaggagagag cgagggctgc ggcgagcgtc aaaagggcgg
     3541 atttgatatt cattagtcga agagaggttg aaagccgtat cctaacctgc ttccggtttc
     3601 ttgtcaatga acctgcaagc agtaatttgt tatagctttt tgttatatta tgccatagaa
     3661 taattgtatt atcgaataat aacgctattt gccataagaa gtctgcattt cattacagat
     3721 tgaaaaatat catgaaaaca tcagttgcag gataataaat caacatggcg tttaccgccc
     3781 tcctggggag ggcatgcttg tcttgccgct ttcgatatta ccgggaataa agcagaaagc
     3841 gccgatattt ttctgttcat ccgggcaggt ggaaggacac agtacttgcc ggcctttgcc
     3901 tattagcctt gaaggcaggc attctgcttt tccctttatg gttcaagagc cgtttccctg
     3961 ttgacgggga acggattttg ccattgcagc atgcgccctg cgtcaatatg gacaatgctg
     4021 tccagatctg ccctgtctat ccgggg
//
LOCUS       DAWEMX010000175         4040 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_7889_length_4040_cov_10.970138, whole genome shotgun sequence.
ACCESSION   DAWEMX010000175 DAWEMX010000000
VERSION     DAWEMX010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_4040_cov_10.970138"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          858 a          911 c         1158 g         1113 t
ORIGIN      
        1 ccgttctccc cccccctaca ttctcttagt tagtttcctt cttgtaagat gtcgtggcca
       61 tataattttt tcttaactat tcctttttct ttcttaatga cttaagtatg tgtggggtat
      121 tgacggatta cactctttcg tcagaatggc ggcatatgaa atcccaagtc attcagaaac
      181 ctgtggtcgt cattctgggc aacgccggca atataggata tttggcagcc aaggcatttg
      241 cctgtcaggg aactgtggtt gccatgcttc atgaggccgg tgaagaatct tgccatatcg
      301 ttgattgtct tccgggcagc agccacctgg tcatgtcagg ccgcctgaac cattcttccg
      361 atttggcttc ttttgctggt ctggtacata ctctttatgg acgtgttgat tatttgatta
      421 tttgtgaaga tgcctcagtc gaggtgcagg gactgagttg ttcttcccgt gtccgtctgt
      481 ccgttttgct ggaacatgcc tttgtttctg gggccatgat tgtgaatatg gtttcccgtt
      541 ctggaaaaaa gggagtatgg gtggaagata tcgcgggctt ggaaaattgc ctgaaccgga
      601 gagatgtcag aatgagccag atcgtgattc accgtccggt atttcggtat gtttcccagt
      661 ctcgattggc ttcttccgcc tgtgcggctg cgttgttttt atgtgggaac gaaggtcgcc
      721 atattcaccg ggaatatatc gtggtcaatg gaaggaaagc tgcctggcgg cccggcaggc
      781 ggccggaatc attcctgtcc ctgtttaatt cctgcctgga gttttgctcc atttgttcac
      841 gaatggtatt tcaggatgcc tgacaggcgt gccatatttc tgtattctgt cgtgacaaaa
      901 agtgtctgtt aagatagtat atttccaagt aaggttatga agtttcaagg tctttataca
      961 gcaattgtga ctccgtttgc cggcaaccgg gtcgatgaag aggcttttag aatgcttgtg
     1021 gaagcgcaga tagctgccgg cgtggccggc attgtggctg tcggtaccac gggagaatcc
     1081 cccaccttga ctgtaaaaga acacttgagg gtaatagaat tggccgtgga gatcgcggct
     1141 ggccgtattc aggttattgc gggaacgggc gctaattcca ccgcagaagc tatccatatg
     1201 acccaggaag cggaaaagat gggagtagac ggaacgctcc aggtgtgccc ctattacaat
     1261 aaaccttctc aggatggagt ttatgcccat ttcaaggcta ttgcggacgc tacccgtctg
     1321 ccgattatgc tgtacagcat tccggggcgt tgcggtattg aaatcagtgt ggaaacaatc
     1381 agccgtctgg cgaaggattg cccgcatatt gtttgtgtga aagaggccgg cggcaatgtg
     1441 gaccgcgtga accagctgat gcaggtggtt ccggaaaatt tcactgttct gtgtggcgat
     1501 gacggtctga cggtgccttt tatggcctgt ggcgccagcg gattggtttc cgtaacatcc
     1561 aatctggttc cgggcatcat gaattccatt gtcaaagctg gcctgaatca ggatatgggg
     1621 gaaatgcttt ccctccagaa gaaattttat ccgttgatga aagggctaat gactctggat
     1681 tccaatcctg ttccgatcaa ggcggctttg gcgatgaggg gagatataca gcccggcata
     1741 cgtcttccgc tggttccatt ggcggaagag aaggaagctc agctttccgc tcttcttcag
     1801 cgtttcaata ttctttaaca ttttagtgat atgatttcca ttcttgttac aggcatttcg
     1861 ggccgcatgg gccaagctat tcaagaggcg gtgacgcaga atccggatac ttgcgtgggt
     1921 tctactcatg atcagggaca ggagctttat cccgccctcg ccaaatgcga cgtagccatt
     1981 gatttttccc atcacgcttt caccagcact ctgctggcgg aagctgttgc caataacaaa
     2041 cctctggtga ttggcactac ggggcatacg gagctggaac gccaggagat cgtggacgct
     2101 gcggcatcca ttccgattgt gttcgcttcc aattattccg taggggtgaa cgccctgttc
     2161 tggctgacgc gcaaggctgc acagattctg ggaggcagct gcgatataga ggtaatggag
     2221 atgcatcacc gtcacaaaat tgatgctcct tccggtactg cccggacact ggcggaaatc
     2281 ctgtccggcg ccattgacag gaattatgaa gatagtgtgg tttttggccg tgaagggttg
     2341 gtagggcctc gacccgccaa agaaataggc atgcattccc tgcggggagg ggatgtggtg
     2401 ggagaccata ctgtaatatt tgcttccgat ggtgaacggc tggaacttac ccataaggcg
     2461 tccagccgta tgacatttgc ctctggagca gttcgtgcgg ctctctggct ccaaggcagg
     2521 gaaccgggtc tgtataccat ggaagacgtt ctggggttgt cccaattata actttttcgg
     2581 catcgtggcc gttcttcttt tccggaacgg ccacgatgtt ttttgctgat gaagagagca
     2641 ggcattgcac tggggtccaa tcttggggat aaaaacagcc tgatggttca ggccagggat
     2701 catttgttgc agatgagcct gtctgatgac ccttttttgc aatcctccct gtatgccacc
     2761 agtcctcagg gatgtccgca cggtgcggat gattatttga atgccgtggt ggaatttacc
     2821 tggccaggta cggtggagga attgcttgtg cattgccagg gtatagaacg ggttctgggg
     2881 cgcattcgtt ccgggattcg ctgtgaaccc cgcacggcgg atgtggatat tatctatgtg
     2941 ggggacatgg tggtcaatga tcctcctcgt ctgattctcc cccatcccag agcccatttg
     3001 aggaaatttg ttttaaaacc tctttccgat atcaggccgg agcttatcct gccgcgccag
     3061 cataaaacgg tggcctgcct gttggcggaa ttggatacgg atgaggctga tccgctcctg
     3121 gtatcggaac gctggtaaat tgcatgttta ttgaacgcca tgaatcaatc ttttgagaaa
     3181 gcggaacgaa tccgcgcctg caagaacagg cggcatgtga ctctggttac tgcttatgac
     3241 tatcctacag gccgtctttt ggatgaggcc ggcgtagata ttgtgctggt aggggattcc
     3301 ttggggatgg tggttttggg ctttccggat accactcatg tgacgctgga tcatatgctt
     3361 catcatgtgg aagctactgc gcgcggagtg aagaacgccc tgctggtggg agatatgccc
     3421 atacattctt atgatacggt ggaacaggcc gtgcaatccg caaagaaatt gtttttggct
     3481 ggagcggatg ctgtcaagct ggaagggggg gcagcccagg caggaaatat ccgtgctatt
     3541 gtggaggccg gtattcccgt agtgggtcat atcggcctcc tgccgcagaa agtgctggag
     3601 gagggaggat ataagatcaa agggaaatcc tccgcggagg aggaagcctt ggtaagggat
     3661 gtggaggctg tggcagaggc gggggcttgt gctgtggttg tggaaggcgt aacgcctcat
     3721 gcggcccgac aagttaccgt acattcttcc attcccacgt tgggtattgg gtccggctcc
     3781 catacatgtg atggcgatgt tgtggtgatt catgatctgg tgggcgcatt tccgtggttt
     3841 attccggctt tcgtcaaacc tcgtgcggat gtcgctggaa gcatgactgc cgctgtcaag
     3901 gagtggatgg aagattgcta cacggaaccc ggctccatca gctcataaca attgattttt
     3961 ttatgctggt attttctacg tgctggaatt cccaccgtca tcaggacggg gaagaaatga
     4021 ttgatgagat tctttctttg
//
LOCUS       DAWEMX010000176         3938 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8085_length_3938_cov_8.473345, whole genome shotgun sequence.
ACCESSION   DAWEMX010000176 DAWEMX010000000
VERSION     DAWEMX010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8085_length_3938_cov_8.473345"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1518..1617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          887 a          940 c         1135 g          876 t
ORIGIN      
        1 aacaagctgc ttaagattat tgatagcgag agtgacagcc tcagaattta tcatatgggg
       61 tccaattggc atcataaaat agaacaattg ggtaaggaga aaagctatga catctccggt
      121 cccttgatta tttaaagact gttgaacatg gcctgtgcgc caacctcaag ctcacacgaa
      181 ttccccggca ggtcggcgat tggtgtaatg cattgggaat agaagattga cagataaata
      241 ctcagaaaga gcaaccgtgc aatgacggtc tttttaggga ggttggcgca aagtatgatt
      301 tgctctgttg agtagcaatg tataaggtcg ggcgcagtcg cactccacaa ggagtgcgtg
      361 gattgaaact gatcggtgtc cttgatgtcc ccgttgtcca ttggtcgcac tccacaagga
      421 gtgcgtggat tgaaaccgga gataccggcg catttcaatc cggagaagct tcgtcgcact
      481 ccacaaggag tgcgtggatt gaaactgcac ctgccttccg ggaacgaggg aactatcacg
      541 tcgcactcca caaggagtgc gtggattgaa accaaaactg cttccggcca ttgaagcccg
      601 tgccctgtcg cactccacaa ggagtgcgtg gattgaaacc attcggaaaa cttgtaaccg
      661 tactgaaaga tgagtcgcac tccacaagga gtgcgtggat tgaaaccgca aacacgaaaa
      721 agagctgatt gccgcccgcg gtcgcactcc acaaggagtg cgtggattga aacttcggac
      781 aatacttccg ggccgcccgg ttccatcggt cgcactccac aaggagtgcg tggattgaaa
      841 cgaaacccgc cgtttcctcg gtttcaatgg cattggtcgc actccacaag gagtgcgtgg
      901 attgaaacat ctactggccc ggcgcttgac gtggcaatac gcgtcgcact ccacaaggag
      961 tgcgtggatt gaaacgaatc gtttaagcag ggggaagcct ggaacctggg tcgcactcca
     1021 caaggagtgc gtggattgaa accatttcca gcagggattc cgctctggta cggtcaacca
     1081 gtcgcactcc acaaggagtg cgtggattga aaccattgtc tccatgcggt aggagaacgc
     1141 agcccaggtc gcactccaca aggagtgcgt ggattgaaac ctgcccctgg gaagcggacg
     1201 gatgatgcgg gtcgggtcgc actccacaag gagtgcgtgg attgaaaccc gtcatccgga
     1261 aatcggcatc atccgcctgt accgtcgcac tccacaagga gtgcgtggat tgaaaccgga
     1321 tgaattccgc aacgaacggg ctgcggtgca gtcgcactcc acaaggagtg cgtggattga
     1381 aacaacatgg tccgggtgcg ggcttccgtc tgaccgagtc gcactccaca aggagtgcgt
     1441 ggattgaaac ttcatcataa tttttcaccg cttctccggc gacggtcgca ctccacaagg
     1501 agtgcgtgga ttgaaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc
     1621 gcactccaca aggagtgcgt ggattgaaac cgggaatttc cggtggatct gtcagcgttt
     1681 ttggtcgcac tccacaagga gtgcgtggat tgaaaccgct tgccgtgggt gcgccgaccg
     1741 atacgtcagc gggtcgcact ccacaaggag tgcgtggatt gaaacctttt gctccgaaag
     1801 ttgcttggcc gggatgttgt cgcactccac aaggagtgcg tggattgaaa ccccatccac
     1861 tcgtaaagct tcgcgccggc aaggggtcgc actccacaag gagtgcgtgg attgaaacct
     1921 tctgacgggc cacaacctcc gtagcggtca ttgtcgcact ccgcaaggag tgcgtggact
     1981 gaaacatgac gagggcaagc ttcgagaaga tgcctcccgc cgtactccct ggagagtacg
     2041 taacttatct ggaaaagaaa aagctctcca agcttgtttg gtctagagag cttttttgtt
     2101 ccatcatcct gttaagttgt caggaagagg ggtgattatt gagattgagt tctgccgggg
     2161 ggagggactg gagcagagcc aggatggggt cctgtggtgc agcgcccatt tcccgctggc
     2221 gtttgaattc ctcctcttcc tgctgcagcg ctgccagttt ggtgtcttcc accagcgcct
     2281 tgaatttgtc cgcctgagag tattccccgt cctcgtcccg gaaaacgcgg gctaaccagc
     2341 ggcgtttttc ttccgtactg agcaggggat ttttcatgtg ccggggaatg tatttttgtc
     2401 cagcaggttt ccggcaagct gttctccatc cgcagtgggc cggtaaacat aagggcgacc
     2461 gtctccaata cggttgacat atccctgcgc aatgagatcc tgcaggatat tggtgacggt
     2521 attagggtgc agccgtgtgg cttccgtaat ggtgctgtgg cgcctgtacc cggcaatgac
     2581 ggccaggatg acctgggcac tgctgaggcg gaagtccagc ctgcggacgt tggaggcaaa
     2641 tatctgtatg aaatccagtg ctttcatgag catgtattca caacttatta ttatgttatt
     2701 tgcttattat tctcatgtta ttcacattct tccgggcaag agggcgattt gatttttctc
     2761 agcaaatcca gcagcgggtg ggaatcattg gaaaagcact gcaccgcatt gacggtagcc
     2821 gcctgcggaa gaatgatttt atggaattcc tggttaagat tctggcgcag gctttggttg
     2881 cccgtcaggg gcatggccag tttttctgcc agccgggtgg ccagtgcgtc catgaagagg
     2941 ggatcgaatt ggtcagcgtt ttcaatatcg gcaatgtagc ttaggggcag cggggcgcag
     3001 gaagcatgaa tgcggcgtcc ctgcattttc cattccgagc attccacatc cagcacgcgc
     3061 aggcagtcta cgggcagcgt gaattgatag ggggtcaggg aattgggcgc ctgtgcggaa
     3121 acggagtcca gagtggtttg cgtagcggcg aaggtccagc gcgccatgca aagggtttcc
     3181 ctgcgggccg gatggtaatg aagaacgcaa aggcgggatg ccgtggattc attggcaatc
     3241 agcgcgtcca gaggtgcctc ccctattttg gagagggccg cgttgcagat gtccagcgcg
     3301 gatggcgtat ccggctgaag taaggattca ttgatcatag cgccctatgt tttggaggaa
     3361 acggatgcgg aatgcatcac gtttggaggc gacctctttg atgccaccaa tggcatgtat
     3421 gcgtccggtt tttcctcagg tgcatttttt gccgatttgt ttatttgata aatgcaagca
     3481 cttgcccgga ttgttcgggc aggtggagag agctcccttt ccttccatgg gaaaagaacg
     3541 ctcccgccgc atggattgtc tgtgaggcgg gcgtccggca tcctctggag ccttatatcc
     3601 ggcagaaggg aaggggaatc agtatcaacc atgaatagaa agcaaaagaa attatgggat
     3661 ttatgaaacc gtccacacct tccgcttctg ctccagagca gaccatcccg gtcaaggcgg
     3721 agagcgtgga acaagaagta ggggaagatt atcaggcccg ggaacggcag aggcagggaa
     3781 tgatttccac cattctggcg cgccgtcatt cttccgccgg gcagggggag gttaatccgc
     3841 ggactctgct taggaaaact ctgggatagg cgttgccatg gaagaaagag ttgcggaatt
     3901 gaattccgtg tacaagtcct tggccgccca gcgcgcgc
//
LOCUS       DAWEMX010000177         3886 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8215_length_3886_cov_10.885670, whole genome shotgun sequence.
ACCESSION   DAWEMX010000177 DAWEMX010000000
VERSION     DAWEMX010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_3886_cov_10.885670"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          738 a         1024 c         1167 g          957 t
ORIGIN      
        1 ggttcaggat ttctaacttc ttgttggcgc ttacctcgaa ggcgaacccc acgcaggtgg
       61 ccagcaggat ggccgccagg atgcccgcgg gctccaggaa gacgctggcc ggttccgttc
      121 cggtgaaaaa ctggtagaaa gccacgccta cggacaggag cagggccacc agcagaattt
      181 tgatgatggg gtctgaaaat ttctccagaa attgcttcca caggggttcc ttttccgggg
      241 gagtcaggat attgatcccg tatctggtcc ggttttccag aacctgctgt tccgaaagcc
      301 cctgatgatg tgctgcgctt ttcatggttc agatgcttct tacctgattg gagcctgtga
      361 gaaaagcgcg gaggaatatt ttcaggagtg atgacgggag ttttttatcg gggcgcagcc
      421 tgaggcccgt gccttgaaag gcggcattgt tgtcttgact ttgactatgt tccggagatt
      481 ctggagcacc tatgtggaat gatctttctc atggattttc cgtgctggcg gatgccctgg
      541 ccttcctttc ccgttcctgg gggttctggg tttgcgcggg cagtgtggcg gggctggccg
      601 ccgtttatgc gtggtggaga aagcggctgg ccctgtgggc gtttgtcctg gcgttccttt
      661 ccctgctggc cgtggtgggg ctggcgtgcc gccaggcggt gtgtcaaaaa gctttcctgt
      721 ggattcatga gccggatgtg caggcccttt ccggaaagta tgaggtggaa ccatctccca
      781 gggaaaggcg gttttttcgc cctccggagt tgaaacgttt tttccaggtc agttcttcca
      841 cgctcctcct gaatgaagac gggacgtgcg agatttcttc ctttccccgt ccggaggcat
      901 ggggcaggtg gattgcccgt ccggaagaga gggaggacct ttccgtgctg gaggaagatc
      961 tcgtttcttc ctgccgcgga acctggtccc tggtgaaaaa aggaggatat tacggtgtct
     1021 ccatcgccta ttcccccggc aggagcctga tgttgtacct gggcggggaa ggtgcggaat
     1081 gcctggtgat gccggtcaac cctgccgatc cgctggaagc ggtgacgttt gaaaaaacat
     1141 tctgacccgg attctgtccg cacgggaaca aggaagggag gcgggaatcc cgcctccccc
     1201 aatatgtttt ttccaacgcc gttccggctt tgaccgggcg tgtgggccgg ctgatttatt
     1261 tgacctttcc cccttcgtac accagcacct caaagggctt caacgtgaag gagatttcct
     1321 tatcaatgtc tacggaactg ccggagaagc cgtccagcgt ccgctggtcg tcataggaat
     1381 ccttgaaggt gatggagccc ttgacgttcg cagggatatc cagaatgctg cgcagggttc
     1441 cggtcaggct cttttcctgg tcggaggaat tgcggagagc cagggtggcc ttgtttttat
     1501 tccatgcggc ccacccgtac acggcccctt cgttagtttc cttgttccac ggattgccgc
     1561 ccacccagtg cacatcgtcc aaaacgtccg cattgcggcg tatccacttg atccccttgg
     1621 ccagttcgtc ccacagaact ccgccgttgg cgctcatcag gtcccgatcc acgtagagct
     1681 cctggaggga agtgccgcaa gccgtggcgc agcggagttc tttcttgacg ttttccggat
     1741 cgcggggcat gcaggccgga ggaccgaatt tagtgaccat caggccgtga gtcatcatgg
     1801 aattgatggg catcagcggg gaaccctgca caaaaacttc atgcaccagg cggtcgcggt
     1861 aggtgatcca tttgtcgcgg ttgtcgcccg ctccgatggt gccgaagtca ttctcctgcc
     1921 tccatacgga atccgcataa cggaaccaga agggggatgc ccaggttcct acagtgcagt
     1981 tgatgtaaag atcccccttt ttcttgcgca gggcgttcag cacgcggatg atgccttccg
     2041 catcctcctc gttgccgggc cccttggcat ggaagtgggt gctgatgccg tcgaatttga
     2101 agtacttcat attgtagtcc ttcaccatct gggaacagcg gcccacaaag gcgtcaaaat
     2161 attccttgtt ggagagctgg aaattgccga tcttgttgtc cggatgcttc acgttccagt
     2221 aagcgagacg cttgcccttg gaggctccgt agccccccac cggacccagc cacgtgccga
     2281 tgccggcctt ttgcctgccg gcggcggcgt caatgcgctt gaagccctgc gggaacattt
     2341 tgtggaaatc ccacaggctg ttgaattcat cccagccgtc gtcccagacg aaggcgtcaa
     2401 tggacatgcc gcgcttctgg aaaaactgtt cctgccagtc tttcaggacg gccaggcatt
     2461 gttcttccgt catgcgcttg gcggggtccg aatcattgtt gcggttgatg ttcagttcat
     2521 accaggagtt ataatggatg aaggggcggt agggcatcac gcgttcccgt tccatatagg
     2581 ccaggaagga gcgacgtttc tggccggggg caaaaattcc gagaacggag gatacgtccc
     2641 atatttcccc gggctgcaag gtggttttac ggctccagag tccctgggcc agccgggatt
     2701 cgtccgcctg gggttcctgc ttttcctcca gcgtgcgcac gggcagggaa aaggtgattt
     2761 cccccttgct ggcaatcggt tcgctcttgg tctgggccca gtagcgcaga atgtaatttc
     2821 ccctggaggg aacctgtacc gtgtaagtgt tgccggcatt ggaatctccg gtgctgccgt
     2881 tgtggaaatc ggagtcaaga acggcccccc tggaagagag gagctgcacg ccggcaaggt
     2941 tcagcttatt gttgcccttg tcccctccct tatactggaa agtgatggta catgctcctt
     3001 tgccgtcaat ggaaaccggg ccttccgcac tggaaacctg ttctccgtac tgctttttca
     3061 gttcctgggg aacattgaaa tttccggtcc atgaagctgt ggaccacttg tccgcattcc
     3121 agatggcggt ttctccccct tccatatttc cggcgcctcc cacggtattg atgcccatgg
     3181 gagtttccag ggcggtgaac gccaggtcat tgacgatgag tgctcctctg gcgtttccgg
     3241 aaacggaggg tgccccggct ttcccttctg ccaggtcata ttgcatcgct acgatgtttt
     3301 tcatctggac gggcttcata cttgccaggc ggagttcctg cctcaggtaa tgggaatggt
     3361 ccctgagcac ggcgcgccag gaaatgcgaa gggttccgtt cagagcctgg aatgttgcgc
     3421 tgacggcttt tcccgggaag cggtcagcca gcctgaaggc cttggggttg ggctgaaggg
     3481 agacgaattt gacgtttccc gctttcagct cggaggattt aatgctccgt ccgtcctgaa
     3541 gattgatgat gaataattca tcccctgcct tggcgacggg gcccagtttg gaagtcattc
     3601 ctccgaagga aagacttccg ttccgaagga taaatttggc cgtcaggatg ttattggaaa
     3661 gagtatagcc ggaagcggat tgttccgcgc tggctgttcc gggagcaggc cccggaaagg
     3721 cgacttctgc gggggatgtg ggcaggcagc ccagcagggt tgccatggca atggttgtct
     3781 tgaaaaattt catgatgaaa taatattaca gctagcggga ggataatttg gggaaggctc
     3841 gttttcaaga agaaggttct ggaaatggaa ggaaagaaaa ttaatt
//
LOCUS       DAWEMX010000178         3876 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8239_length_3876_cov_11.653494, whole genome shotgun sequence.
ACCESSION   DAWEMX010000178 DAWEMX010000000
VERSION     DAWEMX010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_3876_cov_11.653494"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          712 a          938 c         1328 g          898 t
ORIGIN      
        1 ggcttcccgg actttttccc gccaggcggg gaaatccttc ggagccatat ccggggaaaa
       61 ttcgcatgcc ggacgcagtt tttccagcat ggtctggacg atgccccggg aggaaacatg
      121 ccgctggtcg ctccgggatg aatgaagcac cgcccagtct gccgcccgga atttttcact
      181 gggcggggta agggagaagg aaggaagaga aagggatgcc aatagaagag agaaaaaaac
      241 ggcgggctga atagtcatga agccagattg ccataaaaga agtttccatt gcaaggttgc
      301 agtggtggtc ttttcaggtt gtttctgacg catcggaagc ttgagttttt ccgggcaggg
      361 cggtagtctg gccgtcgtca tgatggcggg gggtaagagt cctggaagcc cggaaccgcg
      421 ggtatgcggc ctgggcctgg cttccggctt cggcatgggg gcggcagtcc atcttgccgg
      481 ggtaagggaa gggcgcagag tgttgaagcc gttgagcgaa ttgtggggcg aaggtaatcc
      541 ctggggcgat gtattgagcg gatggattcc agacagggaa ttgttatgca gccgcaggaa
      601 cggccctgcc tcccagctgg cgctgctgtt ggcgcgccag gccgtggagg atgccggatg
      661 gggaaggaag gaattgaggg atgccgccct gattgtgggc agttcccgcg ggaatgcttc
      721 cggatggctg agcccgtggc cgggacggcg gcccatgaaa attctggccg ttcccaattc
      781 cctgcatagc gaacttgctt cctgcgtcag catcgaattg ggaatttgcg ggccttatca
      841 cgtgctggcg agcggctgtg ccgccggcct ggatgccgta ggcatggccg ccatgctgat
      901 gcggcaggga gttgttaaga gagcgctggc tatcggtctg gatttgcctt tgtgccggga
      961 gctgctgggg acgtactggg cctccggcat gctttcccgc aatggtttga atgatcccta
     1021 cggccctctg gccgacggca tgtgcatttc cgagggaggg gcggccatag ccctggaatt
     1081 gtcttctgat ccaggtattt atgtgaagga ttatctggtg aattccgatg cctatagccc
     1141 gctgggcatg ccggaggacg gaaagagcat agccgccctg ctggaggctg ctttgaaaag
     1201 tcgggatgga gcggttcctc tgaccgtttg tccccatgcc agcggcacgg cggggaatag
     1261 cgtgtccgaa cgggctgctt tgaaaagggt ttttaaagac ggactgccgg atttgaggat
     1321 gatgaagccg tggacggggc atgccattgg aggcagcgga attctggagc tggccctgat
     1381 gctggtcttt gccagggagg gggtattgcc tcccaatcct ccctgggcca ccttcccgga
     1441 aggcggcgcc tgttctgcgg aggagttgcc gttggccggg gggcggatgc tggtcaaatc
     1501 cgccgcttcc atgggcggcc ataatgtggt gctgagtctg gctgtggacg gttgaatatt
     1561 gttgaatagt gaacgcttat gaagaatcct gaacggtttt tgtcagagtt gaagtctgcg
     1621 gggctgctgc gcgtcctgcg ggatgtggaa tgcctgcccg gaggaatggc ccgtctggcg
     1681 gatggccgga aagtggtcaa tctggcaagc aacgattatc tggggctggc ccaccatccg
     1741 tccctggcgg aggctttttc ccgtgcggtg cgggatgaag gggccggagc catggcatcc
     1801 cgcctggtga cggggacgcg gcgggaacat tcctgtctgg aagaggccct ggccgctttg
     1861 aaggggactg aagcggcagt cagtttttcc tccggttatg ctacctccct gggagtgatt
     1921 gcttctattg cagaccggga ggatacggtg ctgatggaca agttgtccca tgccagcctg
     1981 attgacgggg cgcgtttgtc cggggcgcgt ttgtctacgt tcctgcataa cgatatggag
     2041 tcattaagga aaaaactgca gcatttgcgg gacgcgaatc cttccggcgg gattctggta
     2101 gtgaccgaat ccgttttcag catggatgga gaccgcgccc cccttcggga aattgtgcgt
     2161 ctgaaggatg aattcggcgc gttgctgctg gtggatgaag ctcacggatt cggtgtgctg
     2221 ggagaacacg gggccggact ggcggaagag ctgggtgttt cctcccggat agattttcag
     2281 atggggactt tgagcaaggc ggccggagtg agcgggggct atgtggcctg ttcccgcgcc
     2341 tgggcggatg tgatggttaa ctccgcgcgg tccctgattt attccacggc tccgcctccc
     2401 gctttggccg ccgccgccct ggccgcggtg gaagtgatct gcagcgggga gggaaaggaa
     2461 ttgcgccgac gtgtttccgt gctggcaaac agtttgtccg ccgcactggg gatgccggga
     2521 aggcctgttt cttccatctt ccctgttgtg gtgggagaga atgatgctgc cctggctgcg
     2581 gcggatgccc tgctggaaca gggctttctg gctcccgcca tccgttatcc tacggttccc
     2641 cgcggaatgg cccggttgcg cattacggta acggctgcgc atgaatccgg acaggtcacg
     2701 cggctgggga cggcgttgaa ggagctgctg catgggaagt aacgcaggcg ttgtttttgg
     2761 ttctccgcat tggagccggt tcctgttttt atgcggggcc tgccggaaat gggattatcc
     2821 tcattgatgc cgccataaaa ggggaagaag gatatctgat gcctggcgga acggaatgac
     2881 gtccggatgt cctgaatggc agagcttgca tcttgacagg gaagttttct tccagtaagg
     2941 ggaaagaatg aaggttccca aaggaatttt cactgaaccc tataaaataa cgtcatgatg
     3001 aatacttctg attcttgttc tccttcgtcc tgctgcagcg gttctccttc ctgcacggcc
     3061 gctcttgttc tcggcattat cgccctggta ttggcattga tcggtttttt cccgtgcctg
     3121 ggctggatta ctctgattcc tgcggggctg cttgccatta ttgcactgat catgagcatt
     3181 gtggctgtca gtaaaggttc gcccaaaggg gctctggttg ttgccattat cgcgttggtc
     3241 ctgtccgccg ttgcgggaat tgtccaggca tggatggcca gcgctaccgt gacttacatg
     3301 gaacagcacc aggattatgt ggaaaagaag gctctggaaa ttcaaaagga gcttcggcag
     3361 atggagcagg aaaacaggga gtcattgagg cggtccgggg aggcggatgg aatgtcccct
     3421 gcggatggaa caggctccca ggatttgtag ttcctgaacc tttttaggaa gatcggcgat
     3481 gaatggcggg cgctcttcat ggcgttcatg ccgtgatagc tgggtggtca ggaaagtatg
     3541 gctgcctgta tgggctgttc tgtcctgcct gttgctggcg ccctccctca tggctctggc
     3601 aattagccct gacaggatga attctctggc ggcttctgtt gccgtgccgc atcgtgaatg
     3661 cccgttgtgc ggcatgacgc gagggtatgc ggaaatggcc cgcggggata tacgcgccgc
     3721 atggggatgg aaccgaggag ctccggtctt gtttatggca ggactgctca atggaggggc
     3781 tgccgcgttt tatttggcat ggtgctggcg gagaagggcg ggaagccggg aaaaggaaaa
     3841 tagttctccc gcgtagcatg ccgcaagggg gggacc
//
LOCUS       DAWEMX010000179         3843 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8307_length_3843_cov_7.808342, whole genome shotgun sequence.
ACCESSION   DAWEMX010000179 DAWEMX010000000
VERSION     DAWEMX010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8307_length_3843_cov_7.808342"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    648..657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          810 a         1000 c         1226 g          797 t
ORIGIN      
        1 tttcccgggg gggaattttg tcacgggaga agacgatgcc cggcacctcg caccggaaca
       61 tgcgctgaat gcgagatttg cgcatgcctt ccggcagttt ttgaagatag gccagctccg
      121 cggagccgaa aacctggata cgtttgttgg caaagtagga gtagaagccg gcgatcgcca
      181 gcccggggcg gttgagggcg ggttcgcaga tgtggcggct gaggccggcg gggctgttga
      241 taagttccag ttgaagggct tccttgtact tgccgtaaaa tttggacagg gtgacgaaat
      301 ccactttctt cactttggat ttgctgaaca taatcctata taaagaaaca aaacacacgc
      361 gagagcaatc aaatattgtt cccggcgcca tgtgcgggct ccattttttg aaaaaaggcc
      421 cgttccggca tggaatgcca cactttaccg gttgatttga agccccaggc atggtctgat
      481 gaaggcatga acagactgga tcaattagcc accttgttga gatttccaag catttccgca
      541 cagaaggaac atgcccggga cgtgagtgat tgcgcggact ggctggtaga caaattgtcc
      601 ggcatgggat tggaagcaaa ggcctgtaaa acgcccctcc atcccatnnn nnnnnnnaag
      661 gaaagcccac cgtgctgatt tacggccatt acgacgtgca gcccgtagat cctgtggaat
      721 tatgggaatc cgacccgttt gaaccggaag tgagggacgg gaagatttac gcccgcggcg
      781 ctacggacaa caaggggcag ctgttcgccc atattctggg cgtggaggaa ttgctccgcc
      841 agaacggagg ccacctcccc gtaaatgtga ttttccttct ggaaggggag gaagaggtgg
      901 gcagcggcag tctttcccag tttatcaagg agcacaggga agagctggcc tgcgacgtta
      961 ttgtagtttc cgataccggc atggccgctc cggacacccc tacgttttcc tacgggctgc
     1021 gcggcctggc cggggcggaa attatcgtca aaggcccttc cgcggatctt cattccggcg
     1081 tgttcggagg agcggtggcc aatcccatcg ccgctcttgc ggaaataatc gcctctttcc
     1141 acgatgaaga ggggcgtgtg gccgtgagag ggttttacga cggcgtggaa cctatcgcca
     1201 catgggaacg cagcatgtgg gccacggttc ccggcatgag caatgaggag ctggctcagc
     1261 tgacgggcgt ggaaaccgtg gtgacggagg ccggtttcac cggagcggaa ggtctgtgtg
     1321 cccgcccgac gcgggaaagc ctcggtatcg gcggaggtta ccagggtgag ggaagcaaga
     1381 cgattatccc cagccatgcc tttgccaaaa tttcatgccg cctggtgccg ggacagcagc
     1441 ctgaaaggat tgaaaccctg ctggaagaac atgtaaagaa acattccccc aagggcgtca
     1501 cggtggaatt caggcggggc catggcggga atgcttacgt gtgcgatccg aattccgagt
     1561 tcggtcttgc cgcccagcag gcgctggaag aggcttttgg ccacaaaccc gtgctggtaa
     1621 gggaaggagg cagcatcccg atcattgaag aaatgaagcg cgtgctggga gcggatgccc
     1681 tgatgctggg catgtgcctt ccggatgccc gcatccattc ccccaatgaa aactttcccg
     1741 tggatttgtt ctccaagggc attgacatga gccagattct tctgcgccgc ctggggcaga
     1801 tcaagcattg acctttttat ggaccagcct gtagccgctg aaatagtcaa cctgcacaag
     1861 acctttaaaa caggtcttgg caaacccgtg gtgcatgccg tgcgggggat agacatgaat
     1921 atccggcaag gggaggtata tggcctcatt ggcccgaacg gctccggcaa gtccaccctg
     1981 atgaaggctc tgctggggct ggtgaggcct acggaaggtt cctgttccat tttcggcagg
     2041 tccagcctgg ctccggacag caaggaggaa gtgggctttc ttccggaaaa tccctatttt
     2101 tacaaattcc tgacgggggc ggaaactgtt tccttttacg gcaggctgtg cggcctgagc
     2161 ggaaaatccc tgaaagagaa agtcagggaa ctgctggagc tggtgggatt ggctgatgcg
     2221 gcggacaggc ggctgggcgg ctattccaag gggatgctcc aacgcatcgg catggcgcag
     2281 gccctagttc agtccccccg cctgctggtt ctggacgaac cgaccgccgg cgtggatcct
     2341 ctggggtcga gggatatccg gaacattatc gaaaacctga aagggcgggg gatgaccgta
     2401 ttcctgtgtt cccacctgct ggaacaggtg caggaggttt gcgaccgggt gggcgtcatt
     2461 tttaaagggc tgctcattgc ggaagggtct gtgaatgagc tgacgcggga ttccgacaag
     2521 caggaaatac ttctggaaca tgcttccccg gaattgatgg aacggctgaa ggagatggtg
     2581 aaggaagacg gccgtgccgt ttggctggag gatgggcatc cgcgcaactc cctggaaagc
     2641 gtatttctaa aaagtttgtt ggaatggaag gaaaagcatc ccaatccgga accgtaagcg
     2701 gtccggcggt tgccaaacgg gaagtgcgcc gcatgatacg tgaaagggtg ggcgaattgg
     2761 atgccgccgc ccgggaagcc atgtcccgtt ccatctgccg ccgtgtgtcg gctctggtgc
     2821 gggagaggaa tttccagcgc gtggctgttt ttgccgccag gccgggagag gtggatttgc
     2881 tttccctgct ggatctgctt ccggatcggg aatggtactt cccgctggtg catgagggga
     2941 gaaggctgag ttttcaccgt gtatgccatc acggggaatt ggtggcgggc agctggggca
     3001 tacgcgaacc cggtccggga tgcccggaac tgcatgctgg ggaaatggat ttgatcgtgc
     3061 tgcctggggc cgcatttacc aggggcggca agcggctggg gtatggcggc ggattttatg
     3121 atacgctgct ggccggcccg gctgctgggg tgccgctggt gggagtctgc ttcccctgcc
     3181 agcttctgga cgatctgccc atggaagccc atgacaggca tgtggacatg gtgattgcgc
     3241 cgggagggga gtaacaccct tccggagaaa gggaaagagg ctagatacgg aaccggtgtt
     3301 tctgcacggt cagtttataa aacaggtcgt agcgggcgtt gagcaggggc tggaggtatt
     3361 ctacccctcc ggctacctgg ccgcggatgt ccgcatacag ttttcggtag tcggaaagtc
     3421 cgctgatcat ggcttcatac aggggggtgc gcaacccgca ttccacggca atgtgcattt
     3481 ccgccgctgc ggccacgatc tggcgggaca ggcggaactg catccaggcc agccccaggt
     3541 tgccgcccac ccagacggcc agggccgcgc ccacgcaggc gaaccctgct gcgtgctctc
     3601 cgaaacagcc cagcagggct cccagaatca ccagaaggag catcagcttg atttccgtcc
     3661 tcatcacatc ctcctgatcc agaaaaagct tgctgaggca cggcttgatg ccgaagtcct
     3721 tcaggggcat gtaaatggaa cgtcccagcc tctccttgta cacttccaga tccgtctgga
     3781 gctttgcggc cgggaaatcc cctatgtcgc agtcccattg ttccttcacg cgcgggcgca
     3841 cat
//
LOCUS       DAWEMX010000180         3719 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8578_length_3719_cov_10.938865, whole genome shotgun sequence.
ACCESSION   DAWEMX010000180 DAWEMX010000000
VERSION     DAWEMX010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8578_length_3719_cov_10.938865"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          715 a          990 c         1043 g          971 t
ORIGIN      
        1 gttctggggg tgggacccta aagaaaacgg tgcgttaatg acactccttt ggtgctcctg
       61 ctccctccat gcccgacttt tacacgtctg ttcctgtcaa ggcttcctcg ttcttgcctc
      121 gctcggaaat atcgtggcag cctgggggtg gttcggtgtc aacctcatgg ggattggcct
      181 gcactcttac ggttttgtgg aaggcggctg gttctggttt ttcctttttg tgggccttca
      241 ggtccttgcg gcggcttcag ccctgattcc ccaaaggagc cgctgatgat tttacgaatc
      301 atcgggcagg gaaggggagg cgttcgcgac atacggcatt gatgaatgga aaatacggaa
      361 ttgcaatggg agaaccatcc gggagcacct gcaatatggc agcagctgtt tcggcaaaat
      421 ccgggaatat ccagtgcggg agtgtgtatt gatgcagcct tgcgctcgga atgcgtgtta
      481 tttcccatat ggaaatacaa agccataaat gaaaatatga gaaaaaaatc attttcaggt
      541 cttttccata atacgatgcc tgtgaaggtt gataaggccg cttttcgtct ataaaatcaa
      601 taaaaaatag tgctttttca aaacaatcat ccagaatggg gctgtcatga ccgtctcccc
      661 cgcgtccggt aaagaaattc accctcgttt gccctgggtg gagattgcac ggctgctggc
      721 gacgctggtg gttatcatgc agcacgtccc ttccatgggg tttcctccca accaatggct
      781 gattggtccg gcgctggcga cgtttttcct gctggcgggg tatttttccg cctccggcct
      841 tgatgaacag ggggccggaa cgtgggcggg gcgccgcctg ctggttttgc tgcggcctta
      901 tcttttctgg tgcgcggcct attggctggc ggcgggcatg cctcttgccc ctggttctct
      961 ggcttccgtg tttgggctgg gaacatgtcc catgctgacc ccgatgtggt tcctgagaga
     1021 cttgatgatg tttaccctgg ctgcgttttt gctctcccgt ttccgtccag ctctttacgc
     1081 ctttggcctg ttttgcctgt ttctgcaccg gtgggacgac tccctggcat ggcccagtcc
     1141 gtacatgttt ggggattttg cgctcggcgt tatgctggcg tccgccgcac cgggctgtct
     1201 gaaccgctgg ggcaacatgc cccttgccgt gcatggttcc attcttttag cctccgtggg
     1261 tcttgtctgg gtaagctgta cggactcttt cctgattgcg gacgactctt ttcccggatt
     1321 gattgttctg gccttcctca gcttcggaat tattgtaaag gccgtcagcc ccggctggtc
     1381 ggaaaagctg gcccggtggg catccggcag ctttttcgtg tattgttccc atatttttgt
     1441 tctgatagcc ctgatgggcg tagagagctg ttttccctcc ccatggcctg cctgggcgtg
     1501 gtggtgcctg gttcccgcgg tttacatgat ggcgcggagc gtttacgtat tcctcaaacg
     1561 ctatttcccc cgttccctcg tcctgatgag cgggggaaaa tgacctttgg aacaacggac
     1621 ggaaagctcc gccgggagtg ttccatgttt acttttatgg caccggagag acgtcatcct
     1681 gtttctctct cctgggtttg gcataatagc ttgggtcggc aatccagcgg tctcccgctt
     1741 ttttcaccgg aatgaagctg actccccacg actggaattc tcctgtgtac ggttgttcta
     1801 tcaggatcct gttttccccg gccttcactt gaaagggaac aggaggccgg gcccaccaga
     1861 attcctcgtt gtcaaagggt atctcattag cgggcgaatt ccatgtatgc ttttcgtaac
     1921 gccgctggcc tgccagtttg taagtctggg gatttttcat ttccttgccg tttacccata
     1981 ttctggtgcc gcactgggac cactccccgg cagccggtac tccggaacag cgccgtgtgg
     2041 aacgggccgg agcgtcaaac cccaccatag cgtgcatggt gccctccacg ggtgagtgaa
     2101 acgtcgtctc cgcccagacc gtattccccg gttttgtctt ggaaaataga ccctccgcac
     2161 ccgcacgggt acggaaatac aaattgcctc ctcgcgtctg gacggaagac agtcctgcgg
     2221 gagatttgcc cgccagcagg ttatttctta cttcttcctg cctgtcctgc ggaacggggc
     2281 cgaataccgt ccattccacg aaagaatccc tccagtaagg gaaaggcctg cttccggcca
     2341 gggcttccat gcgtttttca aacagagaga aggcctgata agcctccgtg ttcttttccg
     2401 gcagactgcc ggcaaagcgg gaaccatcct caggaattcc tttccaactg cgttccgcca
     2461 tagcgaacat ggcaggccac tgggggctct ggacaggaat gttttttttg ttctccaccc
     2521 gggtatccgg ccatagacac tggatgacgc ccagggaatg gttgtttccc ctggctgttc
     2581 cgcagggctg gcggaagaaa ttcctgcgca ccagaatgcc ggggtcaaag gaattgacgt
     2641 atcctgccgt ggagtcgaag tagggattct tctgcctggc tggatccggc aggcctacgg
     2701 aattttcatc attccacagc tgggcataac tgtctccgga cacaggcagg tcattgttgc
     2761 ccgcccattg gatgggctgc cgtcccaggg atttgacctt ggccgccatc cggtccgcaa
     2821 actgctttcc gttgggaatg cggatttcat ccgctccgat atgaaggtag ggagacattt
     2881 ccgccggaat ttccctgcaa aattcttcca gcagttcttc cagaatgttg atgcctctgg
     2941 gatcatgcat cgggaagcca aagcagtttt taaaataggt gctgtgcccg ggcatatcga
     3001 tttccggaat aatctggaca tggcgctccc tggcgtagcg gaataagtcc cgtatctggt
     3061 cgtaggagta ggtgccgtta acgtcccgtc cctgaatcct cttggaggga tcattcaaaa
     3121 cagggtactt tttgcactgg atgcgccatg cggggtaatc cgtcagatgc cagtgaaaag
     3181 cgtttatttt cagccgggcc atttcatcaa tatccgcctt gagtgtttca acagtccgga
     3241 aatggcggcc tgcgtcatgc atgaagcacc ggatggcgaa cgcggggctg tccttgatgt
     3301 cgcagcacgg gtaatctccc ctggctccca gctggtccag cgttctgcgg gcataaaaaa
     3361 cgcccgcatc gtcagcggcc ttaatcagaa tgccgttggg gcggatccgc agttcatacc
     3421 cttccttgcc cagcgtctta gacagggacg cgtttttttt cacggaaacg ggagcctttt
     3481 tagggcatgt tcccgttttc cagtccacct gggacgggaa gggaatcagt gggaccggag
     3541 cggctttttc ctgctggaga gggtgcgggg atgtccagtc tccatgggag gaggtaaaac
     3601 aaagcatcag gccggaagcc agcaaaggcg cggaaaggga taatcgcatg gcaaatatta
     3661 cacgtggatg atgaatgatc tttcaaagaa acagggaaat tgcaaaagaa aacatttta
//
LOCUS       DAWEMX010000181         3701 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8623_length_3701_cov_10.398519, whole genome shotgun sequence.
ACCESSION   DAWEMX010000181 DAWEMX010000000
VERSION     DAWEMX010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8623_length_3701_cov_10.398519"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          705 a          969 c         1209 g          818 t
ORIGIN      
        1 gttcggagga ttccggaatg ttcaagcttg ccgacagggg aggccgcatg gctgcggatg
       61 agaccatggc catctggttc cagacgaatg aagacgggaa ggtccagtgc ctccgtctgc
      121 tcatttccgg atggaggaag gatgaccggg cggatgattt gagggtgctg attcagggaa
      181 tgcggcattg cgccttggaa cagcgccagc atccctgccg gattttgaat attgaaatcg
      241 gcgccggctc tttcgcggca tcccgggatg agtgtgtgct gaaaggatag gaaaacggat
      301 tgtcccagat accagtttat cctggtttta ttctgaagac gccacattcc ggttgggaaa
      361 tccggctgta tgtccggcat gcctggcccg gagttttttg gccgtcattg acgatgggaa
      421 ggttgtccgt attccgggcg aggcgaaggc gcaggaggaa tggtgcgcta cctgccggga
      481 aagggagttt tgtttttcct gcatgaaaaa aaaacccgga tggaatgatt gccatccggg
      541 ggggggttag ggatccggct attttttagc aggcagtttc acctgaatgt gaatgtcgcg
      601 cagctgacgg tcttccgcgg cggcggggga gtcgctcatc agatccgctc cccggttgtt
      661 tttcgggaat gcgatgactt cgcggatgga ttccgcgttg caaatcatca tgaccaggcg
      721 gtcaaggccc agcgcaaggc cgccgtgggg cggagcgccg aagctgaaag cgtccagaat
      781 gtggccgaat tgttcgcggg cctgttcttc cgtgatgccg agcgccttga acatggcgga
      841 ctgcaagtcg cgttcatgga tgcggatgga accgcctccc agctccgtgc cgttgagcac
      901 cacgtcgtag gcctgcgcgc gaaggtctgc ggagagttcc ccgctgagca gtttttgttc
      961 gtcttccttg acggggcggg taaaggggtg gtggatggcg acccagcgct gctcttcctc
     1021 attccagccc accagcggga attcaataac ccacagaaag tcgcgttcct tgttgtcctt
     1081 gagcagttcc atgaattcgg cgcattgcag gcgcacgcgg cccaggatat cgcaggaggg
     1141 ttcccacggg ccggcggcaa agagaacgag gtctcctgtt tcgatgtcca gggcttccgt
     1201 gagtttttgc ttttcttcgt cggaaaggaa tttggagatg gggcttctcc agtcttcctc
     1261 gcgcaccttg atgtaggcca ggcctttggc tccggcttcc acggcagttt tcgtcagggc
     1321 gtcaatctgg ccaacggagg cggaaccgaa tcccttggcg ttgatggcct tgaccacgcc
     1381 gccgttgctt acggcggagg cgaatacctt gaagccgctg ttttcaaaga tggaggagac
     1441 gtccgtcagc ttcatgccga agcggcgttc cggcttgtcg gaaccgtact gatccattgc
     1501 ttccttccag gtcatgcggg ggaagggggt gggaatgtcc acgcccaggg attccttgta
     1561 cacgcgtttg agcaggcctt ccaccaggtt gtagatgtct tccggcgtga tgaaggaagc
     1621 ttcaatgtcc acctgggtga attccggctg gcggtccgcg cgcaggtctt cgtcacggaa
     1681 gcagcgggca atttggaaat agcgttccat gccggctacc atgagcagct gcttgtattg
     1741 ctgcggggcc tggggcagcg cgtagaattt gccgggcgcc aggcgggagg gcaccaggaa
     1801 gtcccgtgcg ccttccggcg tggatttgga gaggatgggc gtttcaattt ccagaaagcc
     1861 gtgctcgtcc agatagtcgc gcgtggcttt ggtgacgcgg tggcggatct gcatgttgcg
     1921 ggccatggcc gggcggcgca ggtccagaaa gcggtatttg aggcgcaggt cttcgttgga
     1981 gagggcccga tccagctgga aggggagcac gtcagccttg ttgatgacgt tcagggtgtc
     2041 tgcggagact tcgatttccc cggtggggag gtccgcattg gtcgtgtcca ccgtatccgt
     2101 tttcaggcgg gcggccacgg tgccggaaat ctggatcatg tcttcggagc ggagctgctg
     2161 ggaggctttc gccacatcct gattgacttc cgggtgaaag actacctgcg tgatgccgga
     2221 gcggtcgcgc aagtcgataa atatgacgcc gccatggtcc cggacggaat ctacccagcc
     2281 gatgagggtg gtcggcttgc cgatgttcgc agcgcgcaat tcgctgcagg tatgagtgcg
     2341 gtatgagttc atctttattg aaaatggaag aaggggaaag aacggtgtca attgcttccg
     2401 gtgagtgaag ccacggcgtc aaacaggtcg tccatggcga cgggggatga ggtgcgcgcg
     2461 cccaggtcgc gaagttccat gaccgggaat tcatctccca cgatcaaggc cgcgcgggcg
     2521 ccggatttga cggccttctg aagctgtccg ttgactttgg tgagcgtgag gggaaggtcc
     2581 acactgtatc cctggtcccg gagggcggag gcgatggcaa gaacttccgc gcggcggctt
     2641 tcagacgcct gaaccatgaa gatgtcgcag cctgcggagg ccagcgccgc atctttcagc
     2701 gccctggcat ggggcgtctg ttcaataagg tgggtgatga cggcatcccc catggcaaag
     2761 ccggttgccg gcatgtccac tgcgttgttg gaaagggcgc ctaccagcgt gtcgtaacgt
     2821 cctccgccgg ctacggcgcg caggctgcgc tgtgtgtcaa agatttcaaa aaccagaccg
     2881 gtgtagtagg ccagtccgcg cactacggat aagtccagct tgacatagcc gtccaacccg
     2941 cgggccgtca ggtctttcat caggatgtca tagcgttcgg acgctccggc gggcgggttt
     3001 tcaataaagg ctaccagatc gccgcggctg atatgaaagg cgtccagctt gcgctggcat
     3061 tcttccgggc ggtcacgttc aaatttgtcc acgatgccga ggaaagcggg aatatcctgt
     3121 tcctgcacgc cgtgttccga agcaaacctg atccaggctt cccggtcaga gacgcgcaca
     3181 atgaagtcgt tttgcgtaaa gcccagttcc ctcatgcagt cgatggccag ggctatcagt
     3241 tcggaatccg cccacgcgga ggcttctccc agaatgtccg cattgaattg ataaaattcg
     3301 cgcagacgcc ctttctgggg tttttcataa cggaagcatg agccgatttc aaaccacttg
     3361 agcggtttgg tgtattcgcg ctggtaggcg gctgcaatgc gtcccaggga ggcggtgagt
     3421 tccgggcgga tggtgatgtc ccggtccccc tggtccgtga agcggaagag ctgggtgggc
     3481 agctcgcctc cgctcttttt aagataaagc tccgtggctt ccacagtcgg gccctcccat
     3541 tccaggaatc cgtaacgatg cgccaccttc ttccatgcgt tgaaaagata gttgcgcacg
     3601 gcgcagtccg ccggtgcaaa atcgcggaat ccgggaagtg gttgaaagcg agcgtctggc
     3661 ataacctgga aaaaaggatg ttgtctgagg acgaaacagt t
//
LOCUS       DAWEMX010000182         3697 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8633_length_3697_cov_10.910214, whole genome shotgun sequence.
ACCESSION   DAWEMX010000182 DAWEMX010000000
VERSION     DAWEMX010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_3697_cov_10.910214"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    961..970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          828 a         1177 c          907 g          775 t
ORIGIN      
        1 catgccctac atcacggcca cacacgatat caggaatccc cgcagcggag gcgtcatgaa
       61 acagctgggc atgcgctacc aatattctta tgaagagctg tggaaaccta aaaatattcc
      121 ggtcactttc cgcatgtacc agttgaatct ggatggacag gaggatcggg tttatcagga
      181 atactggaac aggtcatccg tccgtttcaa agaaactggt atttgaattc ccaatacccg
      241 gcctcatctt ctagagttcc ttcccgccct gggaaacatt gcggatacct ccgcatccgg
      301 tcacctttcc accggttttc cggagaaagt cccgctccac ggttcttcca gcctgtccgg
      361 aacgctttcc aactcccgga gtattttctc ccgctcccgg gaaatccgct cctgatccgc
      421 agaggccgga acagactgct ccccgggcag aggcatcagc ccgtccgcca tcctgccacc
      481 gtccggggca accggcggag caacaccctt gttgtcttca tccgtttccg gttcaggaca
      541 gaccggcttc tgctcttcca tctttccgga actttccgga acagccaggt tccgatacag
      601 gaacaccagg tcccccgcct ccacagcgcc gctgcggata tccgcaggaa tgcgcgcacg
      661 ccccatccgt tcctccgtca caatcaggtt ggcaactctg catttttctt cccgtcccgg
      721 agattgggag ataagcacgg tccccggttc ataaacattc ccggccagag caatcagcac
      781 ataatgatga ttgggataca cctgctccac cttgcccaca tatacggggg gaggggctgc
      841 ttttttaacg ggctgctgca cctgctggga acaggaagcg cccagcacta caaacgccgc
      901 cagaacaggc agggaaacaa ttcgtctcat ggtgagtttt ttcgtcgttt cattccacca
      961 nnnnnnnnnn ttgcgtggac ggttggtatt atccgccggg gcaataatgc cgcgggattc
     1021 cagcaaatcc atcatgcgcg ctgcacggcc gtatccaatg ctcaaacgac gctgcagcag
     1081 ggaggtgctt accttacgtt ctacaacagc cacttccagg cacttggcat agcactcctc
     1141 ctccgcgtca tccagcggac tgtccgcacc tccgcgggaa ggctcgtcca aggacttctg
     1201 aacctcttca tggaatttct gcttggcctg ggaagcacag tgggccacca gggcttccac
     1261 ttcatcgtca gagataaagg ctccctgcgc ccgctccacc tgggcggaac ccggcggcaa
     1321 atacagaagg tctcccttgc ccaccagttt ctccgccccc tgcctgtcca aaatcacacg
     1381 gctatccgta ccgctcgcca cctggaaagc gatgcgcgtc gggatattgg ccttgatcgt
     1441 ccccgtcacc acctgccttc tgggcgtctg cgtcgccaca ataaggtgga tgcctgcggc
     1501 acgggccttt tgggtaagac gtccgatatt cgtttctatg tccgctccta ctgtctgcat
     1561 caggtccgca agttcgtcaa taataatgac gatgtagggg aagcgttcag ggataactcc
     1621 gtcgtcctcc agctccagtt catcatcttc ttccacaggc cattccccct gggattccag
     1681 gtctctggca atggattccg ccaaagcttc atccacctgc ccgtcttccg gttcctccgt
     1741 ctcttgcgca ggaacatccg gcgggcgctt gttgaaagct tcaaagtttc gcacacccac
     1801 cttggcaaag caatggtagc ggtgctccat ttcatttacg caccaacgca gggcattggg
     1861 aaccttcttg ggatccgtca ccaccggaac aatcagatgc ggcagcttgg aataaggctg
     1921 catttccacc actttcggat ccaccagaat aagcctcagt tcatccggac ggaatttcag
     1981 aagcatggag gaaatgatac tgttgatgca cacggatttc ccggatccgg tggcaccggc
     2041 caccaacagg tgcggcatgg atgccaagtc cccgatcacg gtgcgtccat acacgtcttt
     2101 ccccagcgcc agcggaatct ttttcttggg ggaacaaaaa gcaggatcct gcaaaagttc
     2161 ccgcagggga acggctacct tcttccggtt gacaatttca atacctaccg tatccttgcc
     2221 cggaatgggg gccacgatgt tcacgctttc cgccttggtc gccagcgcaa tatccttcgc
     2281 atactggtca aaagtattta cgcgcacgcc gcgcgcggga tacacttcat aacgggtgat
     2341 ggtcgggcct cgggtaatat ccccaggcgt cacgtccacg cggaatgtcg tcagggtatc
     2401 aataatcttc tgctgtattt ccagcatttc ttccttgtcc tctgccgtag ggccttccgg
     2461 cttttcttca taatgcagca gttcaaaggg aggcagctca tactcgcgga attcctccgt
     2521 gggcggagtg gacaatttgg aaaaaggctg ctctttaggc gcaggcttca tttccgccgg
     2581 ttccgcaacg gtaatcctgg ggcgcggagc caggggaagg cggccctggg tgcgcggggc
     2641 gctgggaggc gggacagaat cctccggctt ggacggagca accgccgccg cttcatgata
     2701 caaactttcc agatcgtccc cggtgcgctg cagacgggga acggaagccc ggccctttct
     2761 gaccggtctg ggagcggaca aagcggcgtc ttccatgctg gccctcacgc gggcagcttc
     2821 cctggcaagc agagcggtct gtcgcgccag tcttttttcc ttcctgttca tacgccaggc
     2881 acgccattcc cgcagcatgg cccggcaaaa cggacgggga cgcatcccga ctgcataaac
     2941 cagggccagc aaatagccca gcccggaaaa agccagcaca gcggatctcc cggccaacgg
     3001 aagaaacaga ccagttccca gcaaatggcc cgccagcccc ccggcgccca gaatctggtg
     3061 gcgcgccacc cattcattcc ccggcacatg cccggccgtc agaaacaggc atccgaataa
     3121 cagcaggaac aaaccgccgt aaaccacgct tcggggcagc cttccatggt ggagcagacg
     3181 gtaaagcccc caccactcca acaggataac cagcatccag gccatggctc ccagcatcca
     3241 ataaaaaatt cccgcaccat ataatcccgc cgttcccagc acattggagg tacccggtat
     3301 aaccagcccg gagccattta aataactcca gcctatttcc cgcacatcaa atgtcagcat
     3361 agcagccaga agagccgccc cccccaacaa agacacggca ccccatgcca ttccaatcca
     3421 aacaggggaa cgcccctctt catgctcaaa tgcatgacgc tcctgcatat ccaatgccat
     3481 acctgtttct atcctagcaa ttcttcgccc caaactcaag gctaaacagg gaagaaaaag
     3541 aatcgcccga atccgcttcc ctcaagacag gaagtgccgg cttacggacc ggaaataagg
     3601 ttttttaaac acttttgcca ctctggaaga gtatcgctag gaattccttt ttttacctga
     3661 gaaaagaccg tccctacacc gcaaatcaat aaaaccg
//
LOCUS       DAWEMX010000183         3690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8661_length_3690_cov_11.104264, whole genome shotgun sequence.
ACCESSION   DAWEMX010000183 DAWEMX010000000
VERSION     DAWEMX010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8661_length_3690_cov_11.104264"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          707 a          950 c         1174 g          859 t
ORIGIN      
        1 ccagtatccg ttgcggcatt cgggcagcgt gcttttggcg gaagactccc agccctgcag
       61 ggattccgta gcgttgcgca ggtaggcttt ctgtgagtcg aacccgatgt ggcgcacata
      121 tttgcggtcc acctgaagca ggccgcccca agcggtttgg agttgaagat tttccgcatc
      181 cagcgccttc agggtgccgt aaacctcgtc cccattggcc agcctgatga tggaaaactg
      241 ttccggtatt tccagcggac gggaagaggc cagctcgtca agctccccca tatggacatc
      301 cacgggtttg gaaagaatgc tggaacggac ctttaggtaa gggcggttca tcgtatccat
      361 gctgcccggc agtttttccc cggatttaag cgtaatgacc ccttccgaac gagcatgggg
      421 cagaaggagg gcggtcggca gggcgcaggc gcacaggaaa agcgtaattg cctgtttcat
      481 ggcgggatca atcagggagt ggaacgggat ggctgggcgg gattcaggaa ttccacgcgg
      541 gtgatgtttt tcagcggcag ggaaatctgt ccgagctgcg gatgccgcag gatgatattc
      601 agggaatcca tgctcagaat atctccgtgc aggcgggaac cgtccttcat ttggaggctg
      661 tggcgggctg gggggcggga gtcgtctttc ctgttctgtt ccgcaaaaaa gacggtatcc
      721 agatacttgg ccgggatgga aagatcacgg ggaatgcccg gataatcctt gtccgtggaa
      781 agcgtcagcg ttccggtttt actgtcgtaa ccgctgacgg aacccggaat ggcgtcttct
      841 tcctttgtca ggacaaggtc tgtctctgca ttcccgtttt tggccacgga tttggcgctc
      901 agggtgatgg aggaaagggc cagtttgctg atggtcaggg acacgtgccc ttccatctgg
      961 aggccgaagg cgtctccctg taatggctgt tccggcgggc tggggtctgt tatcagtctg
     1021 ggcggcagat cgcctatcaa cagctcgtaa acctgcttgt ttccaccctg gcgggaggct
     1081 gtcaattgaa tatccacgcc ctccgtcaga atgggggccg gaatggccgc ttcccccagc
     1141 acgaggatgc cggatttctg ttcccggagc agggtgcatt ttccgggcga gatgctgagc
     1201 atgagcctgt tcaggttgct gccggaattg gaccggaaag gaatattttc attccccccg
     1261 aaacagaaga caatgccgga gcggtcatta ttgccgtcca tggtgatggt ggtgcggaag
     1321 gtgaaggagg aaggatccag gccgatattc ttgtacaggg gtatccaggt ggggtaatca
     1381 ttgttgccca gccggtactg gtacttgtct ccctggactt tcggcttcag ggcattcatc
     1441 agttttctcc ccgcctgggt gcggtagtct tcctcccggt tgccggaata aacccagctg
     1501 ttgtcaaaaa tcagcggttc ctgccagaag cccttttcct gcagcgtatt cagccggaac
     1561 ccggccacct ggctgcgggg aacggaacgt tcatggccca gcatgtcccg gtactgcact
     1621 ttgtcctgtg tgatggaaat gatattgcac gggataatgt ccctgtgccc cgcagccatg
     1681 aaaattttgt cccgggcgcg aagatccgga gaggcggttc ccgtaaagta aagagcgcgg
     1741 attcccgacg ggagaatgac ggcgttctgg cgcatggccg ggtgcttgat gacgatgccc
     1801 tgtttcccgt aagaaatgat ttccccgcgg aggcggtaat tgttatccgt aaccacgtcg
     1861 atgacggaat tggcccaggc ggtcagcagg caggccgggg ccagcagcag ggaaagacgg
     1921 attctattgt tcatagatgg gtagaaatct gtaattaagc gcgagggaaa gcagggcggc
     1981 ggaagtggaa tatgccgcgg aatgatcgga aagccaggac ccgttgggcg cctgggaggc
     2041 tcccagatag cgcatatttt tgtaattcca ttccttccac acttcctggt ccgcatggaa
     2101 cagggcctgt gacatgtaat attcaaaata aaacggataa gtggaatccc ggtaattcag
     2161 gtgctttttc aggtaggcca gggaatcctt gaaggacgga tccgttttaa ggcgcgccag
     2221 ggacagcgtc agggaaccga tggccgtgag ggtgacgcgg gggcccgccg gcccggtgta
     2281 gccgtaggca cctttcttgt cgcggcagga agccatgtac ttgaggccgc gttcaaaagc
     2341 ctcgtccggc acgggaatgc ccgcgttgcg cgccgcgaac agggagacaa gctggcagcc
     2401 ggtcaccgtg ctgtctgcgt ccgtggattc cggggaatag cgccagcctc ccgttttgtt
     2461 ttttttctgg gcggtcagtg tcagagctac ggccttgcgg agggcggggc ctatgcggtc
     2521 gtcccgcacc atgccgtagg cttccgccag ggcgagagtg gcgaagccgt ggttgtacat
     2581 ggaatcccca atgtagccgg atacgttgtt ctgcttggac aggatgtaat ctacgcagcg
     2641 gcgcaccatg gtggcgtacg gccccgcgtt cggatcgtct ccgtgagcca gcacggacat
     2701 aaggcaaaag ccgatgatgc cgggctccct cccgtaagtt ccatcatagg tcccatccgt
     2761 tttctgggca ttctgaagat agcgtagtcc cttcacatac atcagttcca cctggggagg
     2821 cacggaagaa gacgttctca tgggggattg ggccgctgcg ttttgcggtg cggccgtcat
     2881 gcagcaggtc aagaaagcgg taagcaggag gcggggattt ttcatggaaa cgggaagttg
     2941 ctgttcatgc gtgaaagggc tgtgtccgga gaggggcgtt ttacctctgt aaagtcttgt
     3001 tgtaggcgtc cagcgccttg cggaattcgg aaggcatgtc gtctgcggac atgccggtgg
     3061 atttgggaac ggaacgcgtt tccacagccg tgtccggatt ggaaacgcct ttatcctggg
     3121 aggaggccat attggatttt ccattcgttc cgcgtccgga attgttgccg ctgcctttct
     3181 gggaaccttc ctgccctttt tcatcccctt cctgctgttc tgcggactgg gcgtcatgat
     3241 ccatgccgag cagttggcgc agcatgtcca taagagctct tcctcccggc ttgggctggc
     3301 cgtcggcccc ggtcatcttg gccttggctg cctggtaaat caattcaatc acatccgact
     3361 gcgcggccag cgtctggcct ccggtattgt aaacttccag caggtccacg gcgtcattca
     3421 tggcatggcg gcattttttc agcagttcca ctacttcctc attggtttct cccaggataa
     3481 ggtcggaagt gtccgcctgg agctcgtcct gttgtactgc cagtttggat gccgtttcct
     3541 tgtgcttggc ggaatattcc ggagcagggg ccggagcggc ggcgcagggc agggaaagtg
     3601 ccagaatcat ggctgggaaa ttggcggtat tcatggggca ggtggggtaa attttttgcg
     3661 gtgttcctgt tcggcggcgc gggtgcgcat
//
LOCUS       DAWEMX010000184         3687 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8670_length_3687_cov_13.666300, whole genome shotgun sequence.
ACCESSION   DAWEMX010000184 DAWEMX010000000
VERSION     DAWEMX010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8670_length_3687_cov_13.666300"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          722 a         1039 c         1126 g          800 t
ORIGIN      
        1 cttgtctcca atctcggcat cggcatcgca gggaagcacc tcatcaagtc cagggacatg
       61 cacgccaaat tcctgtgcgg caccggaaag gcgcaggaag tgaagcagct ggcgctggat
      121 tgccgggcgg attgcgttgt ttttgacaat atgctctccc cttcccagca gagggaatgg
      181 gaacggcttg ttgacgaatg cgtcattgat cgtgaagagg tcattctgga catctttgcc
      241 aggcgcgccc ggacacggga agccaccctg caggtggaac tggcccgcat gcagtattcc
      301 ctgccgcgca tggcccgcat gtggaaccac ctggaccgcc agggcggggg ctccggcggc
      361 ggcaagggag gcggcggagc cgccaggggg gaaggtgaaa agcagattga agtggaccgt
      421 cgtctggcgc gcgccagaat tgaggctatt caaagggaac tggtcctggt gacccgtcag
      481 cgtgccacgc agcgcaagga acgggagcgc caggctgtcg ccacggccgc cattgtaggt
      541 tataccaatg ccggaaaatc ctccctcctg tccctggtgt ccgggtctga agtcatggcc
      601 agggatatgc tttttgccac gctggacacc acaacgcgga agatagagct tccccatggg
      661 cagccgctgc tgctgacgga taccgtgggc ttcatccgca accttccgca ccgtctggtg
      721 gaagcgttca agtccacgct ggaggaagcc gttctggcgg atttcctgat tcaggtggtg
      781 gacgcttccg atccggaagc cgtgcgccat tatgaaacga ctctggaggt tctcaatgaa
      841 ctgggtgcgg gtgacaagcc catgatcgtg gttttaaaca aggtggacct ggtgccggaa
      901 gaacggcgcg gggccctgga aacgctgctg gcgcctcatt tcagcggcag ggtggttccc
      961 atgtccgtga aagaagggaa tggtgaggga gacctgctga atgcctgcgt ggaaatgctg
     1021 gaaagccgtg tccggcgcgc ccggttcctg attccctata cccgcagcga cctggtggcc
     1081 gccatgcata gtgaaggcaa ggttttctct acggaatatg tagaggaagg gaccttgctg
     1141 gaagccgtgc ttcctgtggc cttttacaat aaactggaat cgttcctggc agacaggtaa
     1201 tcccctgttc ctgaagcctg gggctttgcc ttgcgttcca aaggccgttt cggggcggga
     1261 agcgtttttt cttccggaga cagtatccgg attcctggac gggtcaagcc cggtatccca
     1321 ggccgttcag gcatttcatc acatccctga caggcagtcc gatgacgttg tccgtatccc
     1381 cgcgcacgga ggaaataatc atctccccgt gttcctggaa cgcgtaagag ccggccttgt
     1441 ccatcacgtc caccagttcc atgtaatggc agatgtcttt ttccgtcagc ttccggaacg
     1501 ttacttccgt cagcacggcc aggtttttca tgcccagcgg ggaacggatg gcaaccgccg
     1561 tgcagacatg gtgggtgcgt ccggagagct tccggagcat ggaccgggct tcttcttcat
     1621 cttccggctt gccaagaggg aagccttcca gaaaaacgag cgtatccgcg ccgatgacgg
     1681 tggaatccgg atgttcccgg aacacggctt ccgccttggc ggcggcattg tgcaggcaga
     1741 gttcctgcgg gggcatggaa gcctctttca gttcttccgt gccccggaca ataatggaaa
     1801 aaggaatgcc ggttttctcc agcaattcac gccgccgggg cgattgggag gccaggatga
     1861 cgggcggaat catgggagaa aaaagcggtc atgaaaaaca gggagccgat ttttctcaag
     1921 ctccctggat gatgggctgt caatgattgc ctgtcaactg tctgcttccc atgatcctct
     1981 ggaaagcctc gggggaaatt tcccgaatgg acctgataat gtttttggaa aaggcgccca
     2041 tgcgtttccg gatggtttcc cggtcttccg ctgtatcaaa ttccatgccc accagggcgc
     2101 ggcctacgcg ttccccggtg taggtatagt tgaaataaca gagggaagcc agatccgcga
     2161 tgcgttccat gaagtcaagg aaggcgccgg ctcgttccgg aaattcaatg tggacgaaca
     2221 gggggtgttg acacagttcc ggctggtagt gaatcatgcg aaagcgcacg tcgtcgtcct
     2281 cgctgatgtc ttcgaattcg attcctttct ttttcagtgt cgtgcggatg gtatccaggt
     2341 cttcatcgga agcggcaatg ccgaacacgg ggtgctgggt gtttccctcc gttttgccgt
     2401 attgcacatc caccagcgtc gtatcctgcg gaatgtggcg caggtattta agcacctgtc
     2461 cgcgcttgga atccatggaa atgcgcagat agcgcgtttc atggttgccg atgcccgcct
     2521 gccgggcaat ctgggtaagg tgggtgaaat ccatattggc tccggaaatg accacgaaac
     2581 tcttttcctc cggctttact tcgccccggt tccattgttt caggaatcct gccaggctca
     2641 tggctccgga tggttccggc acgacgcggg aggattccca catggcccgg atggcctggc
     2701 agacctcatt attggtcacg gtcacgaact cgtcaaccag ttcccggcaa aggggatagg
     2761 ttaattctcc ggggatgtgg acggctgtgc cgtcgcagaa gacatccacg taatccagat
     2821 tcacccggcg tccctgctca atggaagttt tcatggacgc ctggtcgatg ccttccacgc
     2881 cgatgacctt gatatggggc cagaattttt tcagccagca cgccacggag gcggccaggc
     2941 cgcctccgcc gatggcgacg tacgcgcggt caaaagggcc ttctccgctc atcaccactt
     3001 cgtccgccag cgttccctgt ccggccatgg tcaccaggtc gtcataagga tggacaaaag
     3061 tgcagccgtg ggtttccgtg tattcatggg cggcggcggc cgtctcgtca tagcagtccc
     3121 catggagcat gatcttcaca tgcctgccgc cgtggcggcg cacttccgtc tgcttcactt
     3181 ccggcgtgga gcgcggcatg aaaatggtgg catggcagtt cagcactctg gctgccagcg
     3241 ccacgccctg ggcgtgattg ccggcggaag ccgccacaat gcccttgtca cgggcttcct
     3301 ggctcaatgc ggccatgcag ttgaacgcgc cgcgccactt ataggcccgg atgggcccca
     3361 gatcctctct cttggcgtat accggggcct ggatgcccgg cagattcaat cgctggagag
     3421 gagtcggctc gccaacggtg tagacgcgct ggcgggcctg cagtatttcc gcggaaagac
     3481 ggtccgaaag tatgctcatg attgtaatgc tggcccccat tctactgaag tccaacccct
     3541 ttttccagta aaacgttccc ggggggggcg gcctgttttc cggaattcag gttttctgga
     3601 cgaaaaaagc cgcccggagg cggctgtttc ttctgaacaa ggtggcgaag cggacggggc
     3661 tcgaacccgc gacctccagc gtgacag
//
LOCUS       DAWEMX010000185         3680 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8693_length_3680_cov_9.909793, whole genome shotgun sequence.
ACCESSION   DAWEMX010000185 DAWEMX010000000
VERSION     DAWEMX010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_3680_cov_9.909793"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2303..2312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          736 a         1142 c          981 g          811 t
ORIGIN      
        1 agattgtttt tgcctgaact gtggcatcgt gctaaatact ggcagtcacg aacgcaacag
       61 accatggatc tccatactta tttacgcaat tttccagacg cccagggccg ttttggcgaa
      121 tacggcggcg tttacctgcc ggacgagctg gttcccgctt ttgaagaaat tacggaagcc
      181 taccagacga tagcccactc cgcccagttc attaatgaat tgcggcgcat ccgcaaacag
      241 tttcagggac gtcccacccc ggtttaccac tgcgaacgcc tttcccgcca cttgggcacc
      301 tctcaaattt acctgaagag ggaagatttg aaccatacag gcgcccataa gctcaaccat
      361 tgcatggggg aaggccttct ggccaaatac atgggcaaga agcgcattat cgcggaaacg
      421 ggcgcaggac agcatggcgt ggcgctggct acagccgccg ccttcttcgg cctggaatgc
      481 gaaattcaca tgggagcggt agatattgcc aagcaggctc cgaacgtcac ccgcatgaaa
      541 atcctgggtg ccaaggtggt gcctgtcacg cacggcctcc aaagcttgaa ggaagctgtg
      601 gattccgcct ttgaatctta cctgaacagt tatcaggatt ccatttactg catcggttcc
      661 gtggtaggcc cccacccttt cccgcaaatg gtgcgcgatt tccaaatgtg catcggcgtg
      721 gaagcccggg aacaattcct ggaaatgacg ggacttctgc cggacgccgt ctgcgcctgc
      781 gtgggcggcg gcagcaactc catgggcatg tttaccgcct tcctgggaga cccgctggat
      841 atctatggcg tagaacccct cggcaaaggt cccaggcttg gggatcattc cgcctccatc
      901 acctatggac gcaagggcgt cctgcacggt tttgaaagca tcatgctcca ggatgaagac
      961 ggcaatccgg gaccggtcca ttccgtagcc agcggcctgg attatccttc cgtagggccg
     1021 gaacacgcct atctgcacga catcggccgc gttaactatg tcaccgccac agacgaggaa
     1081 gccgtggacg ccttcttcaa actttcccgt tacgagggga tcattcccgc tctggaaagc
     1141 tcccatgcta tcgcgtatgc catgaagtgg gcacgggaaa acagaggagg cgccattctg
     1201 gtaaactgct ccggccgcgg agacaaggat gtggattacg tcgtggagca ttacggctat
     1261 ggggaagacc accagttccc cgcctgattg ccctgccttt cctcattttc ggcgtatgaa
     1321 gggtgccgtc atccttcata cgccatcctt acgcaaggct cttaacgcag ccacgccacc
     1381 tttccatgaa agaattgtcc cgtctggttc accggagttt tcacattatt ccgcgtcagg
     1441 aagatttccg tcccctggct ttgggagcct ccggccgtac gatcgtccgc atccattttg
     1501 gcggccgcac atgcatcggc atccagtggg gaaatgaccg tgcggacaat gattctttta
     1561 tccccgcagc ccggcatctg cacgcccacg gtgtcaacgt gccggaaatt tacgattatg
     1621 aacctctcgg tcccggatgc ggagccgcct tggtggaaga cctgggagac gccaacctgc
     1681 tggctttccg gaaagaaccc tggccttccc tgcgcctgcg ctacatccgg gctatggaac
     1741 aactacatct gcttcactcc tgcccgtttc cggaggactt ccccctccag cccgcttttg
     1801 acgaggcctt gtaccgctgg gagcagtcct attttgctga acatttcctg ggatcccacc
     1861 tggggttgga aacagcttct ttcctgaacc atcccgccct gaaagagcag gcgcagtttc
     1921 tggcggccct tccggaatgc cccgtccacc gggacagcca gtcccagaac gtgcatatcc
     1981 acgcgggaaa aacctggctg attgattttc agggcatgcg cggcggccgt ccggaatatg
     2041 atctggcctc cctggtctat gacggctacg cccgtctgga accggaacag gcggatgaat
     2101 tgctcaggga gtgggaaaat atctccgctc actccataga tcaccgcatt ttccgtgcct
     2161 gcgccctgca gcggattatg cagatgctgg gcgcttacgc caatatcggc cacaaccagg
     2221 gaaaaacctg gtatctggct cagattcccg ccgggctggg acacctgcgg aagcttctgc
     2281 ccggctccac gcttgcagaa ccnnnnnnnn nngatttttc ccagcccctc tgatgacgct
     2341 tccccccaaa ttcctgctta tcctgtccct gttccagatt tccctggggc tgggctggat
     2401 tctggggtat gtgcttcttc ctcctaccat tttctgttat gaacaggcag cggaacattt
     2461 gctggctggg gaataccgaa ctgttcgccc ggaagggctg aatacggaaa acagcatcgt
     2521 cctccagccc tacgattact tcaacaaagg gcagacggcc cagcttcccg tggtttccct
     2581 gcaccgggaa tcatcactga acagtttcgc ctcctatcct cccaatccca tggattacac
     2641 agtgctgttc cggcacctgt atgaacaggg agccaagaat gtttacgtga tgtcccccat
     2701 gacctgggaa gaaaagccgg acagcattgt caaagccgcc gtggggtatg aactggaccg
     2761 cttcctccac aaggctctgg gccggcaaat gagcgaatcc gcccgccgct cgccccttcc
     2821 gccggattgg aaagacttga ttattcccgc ttccaacatc gtaggaaaga cggaccagtt
     2881 cccgcgcgcg gacaggttcg taggggaacc tccgcaaata accacgtcag ccccctccct
     2941 gggaaccgtc gtggaaaata acgagctgtt ttccccagct ccggaaaaca gggtctcccc
     3001 gccgcttttc atccgctggg gcggggatat cattcccact cttcctctgg tggccgctct
     3061 gaatgccctg gacctgaaac ccggtgatgt cagaatcatt ccgggagaca ccctttttct
     3121 gggcggcaaa cgctccattc ctctagataa aaacggccgc atccccctgg cggcaaacag
     3181 cagccccacc attctggaca cgaaggaagt gatcgtcccc gtcatgtccg gcctgcgtcc
     3241 gccggatact actgccgtca gaaaactgct ttcctctgcg gatgcggtac tcgtttccga
     3301 accatccgcc ctttccgaca cgccggacgc gcaatccctt ctggcggcgc aaactgtccg
     3361 cagcatcatg ggagcgcttg cccctgcccc tgccgtcctg attcccatag cccccgcctg
     3421 ggtacaatgg atcatcgtgc tggacgtcct gcttctgtcc gtatgggctt tgcgttttca
     3481 acgcagaggg cgcttcatca cctggggcct ctgtatcctg ttcgttccct tcattgcatg
     3541 gtacctgttt tccgctcatg atacatggtt ccccgtcatg actcccataa cggcggtact
     3601 gtgcgtagcc ctggcgtgca gtctgttccg ccggcggtgg ggggcctcgc ggggggcggg
     3661 gggggggggg ggggcagacg
//
LOCUS       DAWEMX010000186         3670 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8720_length_3670_cov_10.970954, whole genome shotgun sequence.
ACCESSION   DAWEMX010000186 DAWEMX010000000
VERSION     DAWEMX010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8720_length_3670_cov_10.970954"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          678 a         1066 c         1143 g          783 t
ORIGIN      
        1 gtgccgccgc ccatattcat ccacgggtat ttgttgctca ggtcatagaa gccggaacgg
       61 gggcagatat gaataacggg aagggctttc ccgtccagaa tgccgaccgt cagttccttg
      121 ccttgaatga actcttccac caatacggtg gttccgtggg ttacggcttc ctccatggcg
      181 gggagcacat cctcctgcct gcggacgatg tggacgccca cgctggaacc ctcgcaggga
      241 ggcttgatga cgaagggcag ggggagggac ggcatcacga tgccattgga acagtccaga
      301 atttccgcat gcggcgtagg aactccggct tccagaaaaa cttttttgga ttctgacttg
      361 ttgaagcaca gggcgctgga acgggagccg gcccctgtgt acggaacgcc gcgttcttcc
      421 agaatggcct ggagggttcc atcctcaccg aaagtgccgt ggataatgtt gtaggccagc
      481 tccgtgcctt ccggcagccg gaaatccggg ccgtgcacgt ccaccttcgt tacatcggtg
      541 aatccggccc ggcacagggc gtcgtaaacg gagttgccgg aaacaaggga tacttcccgt
      601 tctgacccgg gacctccgag caggagggcg attttgaggt cgcgcttgag agtgttcata
      661 ttggaataaa taatgaatgg gttaaaactg agggtcccgg tctccgatga cctgggcttc
      721 cgatttcagg tcgatgccgc ggttttcctt ggcctccttg cggatcatgt cgatcaggat
      781 ggtgatgtcc ctcgctctgg catgaccccg gttgatgata aaattggcgt gtacgtctga
      841 tacgcaggcg tctcccacgc aggcgccttt cagccccagt tcatcaatga gcctgcctgc
      901 cgggatttcc ggcgggtttt tgaagatgca gccggcgctg gctccgacgg gctgggaaag
      961 cttccgcctg gcccgtgccg cctccagaag acgctccatt tcttccgcag gggcgggctg
     1021 cgcctcaaac acggcctgaa gcacaaaatt gtggaccagg tccggaatgg aacggtactg
     1081 ggcgttcaat tcttcccttg tatggctgcg gatttccccg tcttcatcca gacaggtgac
     1141 ggaaacgagg tttttgacca tatccatgcc catggctccg gcgttcatgc ggaggcttcc
     1201 tcccacattg ccgggaatgc cgtccatcca ttccaagccg ccaaggccgt tctggacggc
     1261 ggtggaaacc agttttttca accgcacgcc cgctccggcg gtgaggcggt tcccctggat
     1321 ttccagcaca tcgaattctc ccccggaggg gtggatgacg gcgccgcgca gtccgccgtc
     1381 cctgacaatg atgttggaac cgcggccaat gacgtgtacg gggatattcc tgtcccggca
     1441 atagttgacg gccgcctgca ttccggaaaa ggtggaaggc tccagccagt actgggcgca
     1501 tccgcctact cccatggtcg tatggcgtga catgggttcg tacagcttgc cttccagaga
     1561 accgcccgca gtgcgttcca gatcctccag cagggctagg tcgcgcgcga tgcgcatgcc
     1621 gcattcatgg acgttcccgg ctcccagcgt caggaacagg tctccgggtt tcagcgcgtt
     1681 gccgatggtg tggtgggcta tgcccaggtc gggaaggtaa cgggtttcca cggggccgtg
     1741 cctgtgcatg gcgttgatga tcgtctgccc gcttacccct tcaatgggca gttcgctggc
     1801 gggatacacg tccgctacga aaacgaggtc cgcctcctgg agcaccttgc cgaagtcatc
     1861 cgccagaagc tgcgtgcgcg tgtagcggtg cggctggaag taaactacca ggcggttcgg
     1921 ctggagggaa cgggcagttt ggagcgtggc ggctatttcc gttgggtggt ggccgtagtc
     1981 gtccacaatc cggatggagg aggaaaggta tttggtttca aagcgccgtt tggctcccgc
     2041 aaaggaggcc aggccccgcg tcaccgccgg gaaatccacg cccagttccg ttgccagagc
     2101 aatcgcggcc agggaattca ggacattgtg gcgtccgggt atgcccagct ccacgcggcc
     2161 cagttcctct ttccccttca acacggtgaa cagggtgcgt ccgcggcgtt ccagcacgtc
     2221 cgtagcggtg tagtccgcat cggaccagcc gtaggaaacg gaattggggt acttggcgca
     2281 gacatcctca gcgccgggat gcgcgcggca ataaataatc ttgccgcgcg tctgggagca
     2341 aagccggtcg aacacggcct tgatttcatc cagatccttg tagtggtcca ggtgttccgg
     2401 ttcaatgttg aggacgatgc tgtgctctgg aatgtaattt accagcgtgc cgtcgctttc
     2461 atccccttcc gccaccaggt attcgctgtc ttcattccag tgggcgttgg ttcccagcac
     2521 ggggatttcc gcccccacgt agtggcacgg acgcatgcgg ccttccctca tcaggtgcgc
     2581 gcagagggct gaagtagtgg tttttccatg cgtaccggag accacgaccc ctttcttccc
     2641 gttcaggatg gctgccaggc attccgcgcg gcgcatgcag ggaatattca gcttgcgcgc
     2701 tgccgccagg gccggattgt ccgcccggat ggcactggaa taaatgacaa tttccgcatc
     2761 ggaaacggcg tctgcggaat gcgggcagga gaaagacagc cccaggcttt gaagccgttc
     2821 ggtctctgcg ctggtcaccc ggtcgcagcc gctcacccgg tgccccattt ccatcagcat
     2881 gagcgccagg ccggacatgc ctgacccggc taccccgatg agatgtaggc gtgccgggtg
     2941 ttccctgtct gtcagtcttt tgcgtaaggt ttcaatcatg ggaggctgca tggtccgtat
     3001 gttcaatgac attggcgatg cgggatactg catcaggcat gtccagacgt aaagcacatt
     3061 cattcatgga ggccagcagg gaggaatcct tgagtacttc attcaggaaa gccgcaatat
     3121 cgtccggcgt gagggtgttt tcacgcatca tgcgcgccgc gccgtgggcc gcgaaaacgc
     3181 gtgcgttgtg tgcctggtga tcgtctgcgg cgaaggggta gggaaccagc agggcggcct
     3241 tccccatatg ggccagttcc gtaaggctgg acgccccgga acgggaaatg accacatccg
     3301 ctgcggcata agcggcagcc atggcggaac agaattcaat gacgtgcacg tggggcatgt
     3361 ccgccgtcag ctggctgacg cgcgcaaagt ccgcggagcc ggtgatgatg agaaagtcgc
     3421 acaggtctgc gcattgccgg gcggcttcaa tgaccaggga attcaaattc ctggccccct
     3481 gggaaccgcc cattaccagg gccacgcggc ggtcctgggg caggttgagc tccgccctgg
     3541 cttcgttttt gtctttccgg gccaccattt cctggcgtac cggagtgccg gtgacaatgc
     3601 atttggcggg attgaagtag tgcctggctt cctcaatgcc ccgcagcacg ttcgtgcagc
     3661 atttggcggt
//
LOCUS       DAWEMX010000187         3666 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8735_length_3666_cov_10.648851, whole genome shotgun sequence.
ACCESSION   DAWEMX010000187 DAWEMX010000000
VERSION     DAWEMX010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8735_length_3666_cov_10.648851"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          888 a         1087 c          923 g          768 t
ORIGIN      
        1 cattgaaaac cgcatctacc tctccataga agattccgaa acgtgggaag aagtgctcgg
       61 caccattgac cgggaattgc agccgtttgc atcccggctg cgcgccccca ttaccaggga
      121 cgacctggta aggctgacgg aaatcaaaat caagcggatt tccaaatttg acgctttcaa
      181 ggcggaccag cacatccgcc agcttgaaga ggacatcgaa caaacgcaga agaacctcaa
      241 ccagcttacc aaattcacta tccgctggtt tgaagccctg cgtaaaaaat acggcgccgc
      301 ctatccgcgg aaaacggaaa tttcctcctt cggctccgta aaccgcgcgc aggtggctgt
      361 tgccaatgaa acattgtata ttgatgacga aggctttgcc ggttacggcg tcaagaaggg
      421 aaaccctgtc tgcaaatgct ccacgctgga tgacgtgttg atcattgaca atgcaggcgt
      481 gctcaaaatc gtgcggattc aggacaaatt tttcgctggt aaaaatcccc tttatatttc
      541 cgtcatcaaa aaaggggacg accccgtgtt caacctgatt taccgggacg gaaaagacgg
      601 ccccgtgtac gccaagcgtt tccgcatagg agggttcacc agggacaagg aatatccgct
      661 tacccgaggc gcaaagggaa cgcgcatctt ccacttctcc gtgcatgaaa cggaggaaaa
      721 cagttcccag ataagcgtaa acgtttatct gaaagccgtt ctgaaactcc gtaacctgat
      781 caggcccttc cacttcgcgg acctgagaat caaaaaccgc ggtgcccagg gaaatatcat
      841 taccaggcat cctgtggaac gcgtctcccg catcatgcct ccggccaagt ccggaaatga
      901 ggaaacggaa gggccaacaa ccgctccttc cgcaacagcg gaacggacgg aaggctcacc
      961 cgccccctcc gcggaaacgc tgcatcctga aacagcccca catttggagg aaccgcctgc
     1021 cgatccgccg ctggaacagg gctccctgtt tgactcctga aacgttttca atctcttaaa
     1081 atggaaatat ccatgcctga ctatccattt tcggcttgcc acaacgggcc atcttctgta
     1141 gatatgggcc acctcattac catgccgctt tagctcagtg gtagagcacc cgccttgtaa
     1201 gcggacggtc gtcagttcaa atctgacaag cggctccatc ataacccgct caaggtcaac
     1261 ccttgagcgg gttgctttta tgaggcgtgc gccattactc gttcccatga atttccggcg
     1321 tgccgctttt gaacattcat catccattca tatctccggc ggaatgcagc agccgcagga
     1381 agcaaagctg gacgcaacat ctggcaagag gccattccac ccacaggcat ccccgtcaac
     1441 ttccggcagc gaagggccat tcccggagcg ggaagaaggt tccgttggcg ataacgccgc
     1501 ctcagcaagc cgtttccccc cttgaatacg cctccattgc catttcgacc aagggaacca
     1561 tgattcaagc caatggatac tacagaaaaa aagatggccg cggctatcct ccggtttgaa
     1621 gacagccgcg ttaccgggcc ggattccctg cgcgtttccc gccttcccgc cgccgacaag
     1681 ggcggcaagt gggagatttg cggcatttgc gacggcattg aaccggccgt gtttaacaga
     1741 ttgaaggccc tgctggatgc cggaaggcgt gaagaggcct gggaaggttg tctccagtac
     1801 gtcctggata ataccgccgc cgtgcgttcc tggctgggtt ccgacgcttt tcctggcgtt
     1861 gaattcatcc tgcgggacca ttatttcaat tccgggagca ggaataccgg gaagattttg
     1921 cagcgcgcgc tgaatgtcca cggcgccggg cttgtggtgg acggcatccc gggaaaacaa
     1981 acccggcaga tgttgaaaac cgttctcgcc caaacgggag agtccgaatt tctctccact
     2041 ctgaacgaac ggcgcaaatc attttaccat tcttgcaaac agtttcccgt gttcggcaaa
     2101 ggatggctga accgcagcaa tgaggcatac cggttcgcac aatccctggt ttagggaagt
     2161 tcctgcaaaa aaactcccgg aaaaggagtg caggagaatt caaccattgt tgcagcaacc
     2221 tgtttccatg cgccggatgc catgaaagag cctccgtgcg gacatccgca cggaggctct
     2281 ttcattaaca cgatcaggaa aacgcttcgt cccccacaaa cgctcccagc agacggcaaa
     2341 accgggaagc aggtaatttt accaatctcc cgtccccccg cttgccatgc tgggtcagaa
     2401 tccagggcag ggaaagaatc tcccctttat aagccagcaa ttcccgtaca tctcccgcca
     2461 gaaaatggag atgccaagca tcacaccgga ccggctcaaa ccggaagcgg acatcgacaa
     2521 tctttccgaa aggtgcggag gaatccaccc tgcgcgccat caggacaagc tccggagtac
     2581 ggacatagac atcctggcgg cggccgtctt cctccatttc ctccaccctc cggcctgcag
     2641 ccagataata ggaaaaggcg gcagataaag cgttcatacc cagtttttct tatgacgcct
     2701 caaccgctgg cgcaccggac gattcccgtc ggaacggccc ctgaagaacc gtttttccct
     2761 aaaaccgccg gagcaaaaga tcggacggca cccagtcatt tgccctgccg gattttctgg
     2821 ataaccggag gcatctccgt tttcggctgt ttctgaaagc aggacaaatg ccccggttcc
     2881 agcaccaggc aggaatcgtc cctgcatatc tccgcccgct ctgcagtcac gtctacggta
     2941 tacccgcagc cggagaggag agaagacgcc gcataagccg ctccggccag aaccacccac
     3001 aagagacgtt cccaccaggt caatccggga cgtgttccgc ttttttcata agcgtttttc
     3061 atgccctgtt ttcctgcctc cagagccgct ttcttttgtt cttctgtcaa attgaagtcc
     3121 atttgagaga gggggtgagg ggttccagga attctcgcgc atgttgtgcc gcaacggttc
     3181 cgcgggaaaa aatggcgccg cggcaggact gtttattgct attcattttg ttcatttttt
     3241 tgttcattgc ccggaaacgg gcctttaccg gcatggaacc ggcggtgaca tcagaccgct
     3301 cccggacaca gacatccttg ccctgccctt ggggcgggag caacacgcct gatacgtccg
     3361 ggaacaagga atattccgcc agagacaaac gccggaattt ccgccgcgga taacaaatac
     3421 cctgttgaaa acggaaccgg ttcatgctag tctcttccct gctatggcaa aaccggctct
     3481 ggggaaaggc tttgacgccc ttatcaatca aaatctgtct cgtgaatcct tggcggctcc
     3541 ccaggcggga gacgttgtgc atcaactatc ccatgccgcc attattccca gctccctgca
     3601 gccgcgcgcc attttcacgc cggaacaact ggcggacctt gtagattcca tcaaggaaca
     3661 cggcat
//
LOCUS       DAWEMX010000188         3582 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_8971_length_3582_cov_10.343351, whole genome shotgun sequence.
ACCESSION   DAWEMX010000188 DAWEMX010000000
VERSION     DAWEMX010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8971_length_3582_cov_10.343351"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2245..2254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          893 a         1027 c          959 g          693 t
ORIGIN      
        1 cagggaaaaa cacaagatgc cgtaagccat gcggtccagg gattgttccg tttttccact
       61 gatcaggcgc aggggggaag ccagcaggta tatatgggag agcaccgccg ccagcagccc
      121 ggccgcgtac cccagcacaa tggtaatgac atgggtggaa agcaggaagt tggagcgcag
      181 gatggccacc aggggatcca tatggtccac agcctgggag gcttcataca gaatacccat
      241 ctgacaggag aaagccccaa gcaaaagccc cgccgccagt acgattccct ttttactgaa
      301 aagttccacc acaagagcgc agagcactcc catgcaggcg atgaaggcaa tggtttcata
      361 cgtgttgccc accggggacc tcatggtgat gagcatgcgg atgaccagcg ccgcggccag
      421 tactccggtt ccgcccgcgc ctgtcagcca cgccaggccg aaacctccgg gccgaagaaa
      481 acgccgccca aggggagcgt tggcggcagg gcggaaaagc gcacagagca gcaggcacac
      541 gaacgccgcc acaaaaacgg ccagggaaat ataaagcgga tccagacggt agtagaaaat
      601 ttctctttcc aggctgtgcc gctctccggc ggaggcagct tcattgggtt gcgccagctt
      661 ttccagcagc aggccttccg ctttcatccg cagtgcggaa gcctccgccc cttccatgcc
      721 gatagctttg cgttccagta gggacgccat tgccaaaaag gctccggcag ctttatccgg
      781 ggcagccgtc cacaggcgtt ctccatcgcg gccggcagaa ggaaaccagc gtgggaattc
      841 catccgttcc atggcggcag ggttttccag catgatccgg gagacaagca tccatccccg
      901 cattacgtca aagttgcggg aaagggacaa aatttccttc tgggcttccg tcagattcgt
      961 ttctcccaac aaacggattt cccggacggc acgggtcatt tcctcccagc gttccgcaag
     1021 ctgcgcgtag gagtattttt tacgcttgtc tttctgatcc ggcagatgca ggcgcctgac
     1081 cacctcttcc cgattgacca ggaaaacagg gtattgttcc gccagttccg gacggaacat
     1141 gcagtccagc atccattcca ccggggagag cttccttttc ccttccggcg tatcaacaac
     1201 aaagcttctt ttgcccagcg tccggagcag atggaacccc gcataagtag aaaccggctt
     1261 caggcggccg ccgtccagga cggccatggc cccggcccgt tccaccaaag cgggagacca
     1321 gggcacataa tttttcacca gaacaggatg ggcggcagga cgcgctgcca gcatgccgac
     1381 gccgaagatt gccgctacca gcaggcaaat gcctgccaga cgcaaatgtt ttttcccgcc
     1441 gggggcggaa gcggcctgcg gttcagccgt cgccgccgtt ccggcagatg ccagaccggg
     1501 tttgcggcgc aggtatctgc caaaaacaca ggcaaaatgc cacagcaatc ctatagcgat
     1561 gatgtaggag gaccatttgg gcatttgctc caggggatta tgggaagcgc ggagaatgga
     1621 gatgagcctg cccggatgca gggaatcctg gccccagctc atctggtaca gcgtccagcc
     1681 ggaaagacgc aggggctcgt tcatgcggat aacggcgtca tattgaccgc cggattccgg
     1741 aaaaacggtt atctgggatt cataggattt aggcttggaa gtccccggat aaaactccgg
     1801 cacaaaacgg ttcaggcgca aatcaaacgg aagggaatat tcatgcaccc ggtccccctg
     1861 gggcagttcc acctgttcca cggccaccgt cattttgttg cccagcatga taccggccag
     1921 cagcagcaaa attcccccat gagccaccag cacgcccgcg tgcctccaag tccaccggat
     1981 gcggaatacc ccgcctatga gcaggttggc gaacagcagg acacaggtaa tgcccatgcc
     2041 gggcaaaggc agggaaatca gggagttctc cccgcccagc ggtatcagca cataagccgc
     2101 cccgaaaaaa gactctatgg cagcctccgc ccccatggca gaagacagtc gcacctggtg
     2161 cagcgtcccg gcaaaggtca gcaccagaag gatcagcatc agggcaatgc ccaagccgta
     2221 gcttccggca aaattccaga ggctnnnnnn nnnntccgta gtctaaaaag agggccgatt
     2281 gcacgatcag ctcgtgaaac cggccccaat catattcctg gggaaaaggt tattaaaagt
     2341 tgtccgccag ttcttcaaag aatgcctggg gatgtgcaca agctggacat accttggggg
     2401 cagtggaacc ggtatgtaca taaccgcagt tgcggcactt ccactggatt tccttctcct
     2461 tggcaaaaac cctgccttcg cgcacatttt cagccagctt gaggtagcgg gcttcatggt
     2521 gctgctccac ggaggcaatc ttgcggaaag tttccgcaat ggcggggaat ccttccctgt
     2581 ccgctacttc ggcaaacgca ggatacatgt cggaccattc gtcatgctcg ccggcggctg
     2641 cggccagcag acaatcctcc gtagattcca ggggagccgt gcagacggcg tgcgttactt
     2701 caacgcaagc gtctttcagc agtttgaaaa ataccttggc atgttccttc tcattgtcgg
     2761 ccgtttccag aaagatagcg gcaatctgtt cgtaaccggc cttcttggct acgccggcaa
     2821 agtaggtgta gcggttacgc gcttctgact ccccggcaaa agccttgaga agattctttt
     2881 ctgtttctgt tcctttgatg gatttcataa attagtcatc tggttgcgta aacgaaagca
     2941 tcctaataag acgccgtgaa aaaacaagca aatattgaat gaatctaaaa aataaacata
     3001 tccggaaaaa catacggtag ttccccattt gggaaaactg ttgccatgca cgcttctact
     3061 gtcccccata cgcgccagca gcaaaacggc atcccgggaa acaggcagga acccgtaccg
     3121 caacatcagg cctaagcatg aaccggatca tttgacaccc cttgtgcaaa tagtaaaatt
     3181 aaacggaaaa aaccattaag ctccatccat gaattccgcc aataccccct tattggaaac
     3241 cgagagatta atactgagaa aattcacgga aaatgatctg gaggccctgt tcaacattta
     3301 cagcaatgaa gaggtaaaca catatttgcc gtggtttcca ttaacctccc tggaagaagc
     3361 ggcgtccctg ttcaaagaaa agtaccagga agtgtacaaa cggccttacg ggtatcatta
     3421 cgccatctgc ccgaaaaaca gcaaccttcc ggcagggtat atccacgcag gcacggacga
     3481 cagccatgac ctcggctacg gattgcttca ggagttctgg cacagaggct ttgccacgga
     3541 ggcggccagg gctgttgtcg cccggttgaa gaaagacggc at
//
LOCUS       DAWEMX010000189         3561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9017_length_3561_cov_10.916714, whole genome shotgun sequence.
ACCESSION   DAWEMX010000189 DAWEMX010000000
VERSION     DAWEMX010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9017_length_3561_cov_10.916714"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2839..2848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          785 a         1078 c          863 g          825 t
ORIGIN      
        1 tctggtcgcg cagggcgtcc ctctgcgccg tccccagcgc gttgaccgtg ctcccagcca
       61 ctgctgccaa agccctggat gcccccgcca agtcgggatt atctcccgta atcatggtgc
      121 tgatggaatg catcgcctgc ccaagctggg aagtggcatc cagattattt tttgcctccc
      181 acagaagttc ggaacctgct ccggaattat gggaattcac cgcaggcgta aaaatattgt
      241 cctgctgcac cgtggcattc agcacaatat gattgccctc ccggctcatg gtcgcatcct
      301 tgtaataaac ggcaaacagg ccggaagtcc ccacggatat ggattccccc tccgtcagtc
      361 cgtcaatcgt gtccgctgtc atcagcacca cgccgtccag gttgtcatac gttcccagcc
      421 ccgtgttgcc ttccatgttg gcaagagaga atcccgcccc atttccaatg gtgatggaat
      481 ccgccgtcag catagcggag ccgaaaggat cagacgcact taaattataa acgaattccg
      541 tcgtagagcc gctttgaaaa tcaatgctgc cgacgtttaa ggaggtattg gaatctccgg
      601 cctcatgccc aatggcttca atacgcaggg tgccggcaga acctactgca acattacggt
      661 aatcaagcgc gggggcggca gaagtgcctt ttaataccag cgtcccccct ccatgaacgc
      721 ccaggtcata cgtagagctt cctgtctgca ttaccagggt tacgctgccg gatacgtcaa
      781 tacgtccctc tccatccagc gtaccggaga aagaaccgtt tctggcggaa gcggaagtga
      841 cagacagagt gccgccgttc aacgccagcg ttccggaacc ggagagggac tggatggatt
      901 tttccccagt cccggacaga cccagcgttc cgttcagcgt cacggaagtt ccggacaggc
      961 cggcttcccc cgtcagggag agggcggaac cttcctgaat attcaaactc cctgctccgg
     1021 aaatggaggt cgcctggtcc aggacggaat ctccggtagt cttgagtgct ccccccgcag
     1081 caatctccag ggaaccgcct tcatcttcat ccgacagagt ggctgtgaca gtcccaccaa
     1141 tcatggtacg cgcaattgca tctgcagaag cggaggccaa agccagtgtg ccgatattgt
     1201 tgacgctccc gttcagaacc agtgtgcctt ctttggcaat cactgcattg gatgtactga
     1261 cgctcccatt caacaccagc gtcttttctc ctgttttgac caggtccgca ccgtttccgt
     1321 ttacgataag atttccgtta aaaacggaat cgtccaaatc gttggccagt ccaatattca
     1381 aggaaccttc gccggcgaca ttcactgcca ggttgccagt agcgctggtg ccggaggaaa
     1441 gatgacgaac gatggtcttg tccgtattag atgcgggaac atcaaaattc agagaaagat
     1501 ccttatcaac aatcacctgg gcataggaat tcaaagccgg aatttccgtc acagaggaac
     1561 cgtctacgga agcaatgtaa atctggtccg ttcttccgga aatgataatg tttcctccat
     1621 ccactccctg gatgccgaac cccaggcttt ccagcgtggc gttaaacgta agatgctggt
     1681 tgagccacgc caccagaccg ctgtccggca atccggtaat cgtcccgtct gtcagcagga
     1741 taccgacaga atccagttcg cgcatggcaa tcaggatatc cgtatcaata tccatgtgca
     1801 gcgtgagatc ggaagaatct ccgcctaagg aaacagtccc cgtagccgca tcattgaact
     1861 gaatcagact tttctggtct ccgtcagagg catacgtggt attttccacc cccacatgca
     1921 gggcattatt ccccgtcagc gtcaaatttc ccgtcaggcc ggtaatggct gttcccgccg
     1981 tggtggtatt gatgcccccc actttgtcgc cggacaggcc tcccaggtca atgtccccgg
     2041 aatcagcctc cgcatttacg gttacgccgc ccgacgtggc gtaggccccg gcatgttgca
     2101 gccctccttt ggtaatagcg acattattgg caatcgcctg tccgttcata tccaggatgg
     2161 aaccgccgtt gacgaaaaca tcgccagttc ccagggaacc ggtgcccaac gccttcactg
     2221 tgccgccgtt cagcgtagtt ccaccctgat aatcgttggc ggagttcagt tccagagcag
     2281 aggaacctgc agacctgtcc accaccagag cccctgctcc ggtaatctgc cctgtccagg
     2341 agccggaaac ctctccattg cccagctgaa ccgtgccgct ggtaatggtg agcgttcccg
     2401 tcgcgctatt gtcctgtgtt agcaccagcg ttccggttcc ggcctgttcc atcctggcgt
     2461 tcaaggcccc ttctgcgcct acagtggcgt tcatcagtat aatatcgttg gtgtaggaga
     2521 atttaaactg gcttgtccct ccgtcagcct tcaaaaccac tccatccccg aaaagctttc
     2581 catcattcag aactgcctct gtattgttgc cgaatgccca ggtaaacagg gaatcacccg
     2641 tgtgggagaa tgctccctgg aacccggtca tatctcccgt caaatatgcg aacgcattgc
     2701 ccagagccgt ggtcatggtg ccggaaccgg taaacgcccc gccaaagtaa taggaatttc
     2761 cgcttccccc gttcagatag gaacctttct ccagaacatt gattgctgcc atgacggcgg
     2821 tatccgccga attggcggnn nnnnnnnngc gtccattcag ggttaccgtt cctcttccca
     2881 tactggcagc agtggcaaac tgaagatcgc gggcatcatt cgtatttccc ggattattgt
     2941 tcagtccggt cgccaccaat tccccggtaa aggaagaatt atctccggaa atataaaaac
     3001 gggatttggc agcctggttg tccagctcaa tccgccccgc tccgctcatg ccgttgatga
     3061 cgatattggc gttgtctccg gtcgatattc cgttaatggt gctggaaatg gcaagcgtcc
     3121 ctcctgcatt taccgtgact cccgcagaaa acttcgtttg aacactgccg gaagtaatgg
     3181 acacgcctcc ttcatctact gtcaggcccc ccgtatacac ggcgccgccg agagtcaaca
     3241 caccgttccc ggacttttcc aagttcagag ccttgtaatg atctgcagaa gcccagctta
     3301 cgttattacc gttggtatcc actttcacgg aacttttgct cgttgtggaa atctgcccgg
     3361 aaatatccgc cgttacccct gttccatacc ggagggtacc gccgtcaaat gccagatcac
     3421 ctgtgcctaa agcggcatca ttccccattt caagcgttcc accccccaaa atgacctttc
     3481 cggcataatc agcattatcc atattaatgg tcagcttggc actgccggtc ttctccagat
     3541 taccgctgcc ttccagagca c
//
LOCUS       DAWEMX010000190         3499 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9214_length_3499_cov_9.317364, whole genome shotgun sequence.
ACCESSION   DAWEMX010000190 DAWEMX010000000
VERSION     DAWEMX010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9214_length_3499_cov_9.317364"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          699 a          957 c         1112 g          731 t
ORIGIN      
        1 gcgtttgcga tgttgcccac gccggactgg agaggcagga acgtggaggg aatgatgccc
       61 cgtttcatgt cggcagccag gaaatcggcc acgttctgcc cgattttatc ggtcagggga
      121 tccgctgcgg cgaaggaacg ggcttcatcc ggccagttgg tttccacaac gcccacaatc
      181 tttttcgggt caacctggat gtacggcttg ccgatgcggt cgctgggttt gaagatggaa
      241 atttcgcggc ggtagggagg atccaggggt tcgtaaacgt catgcaggcc catggcgctc
      301 ttgctgtggg cggcgtttaa ttccacgata attttgtctg ccaggcggca gatggtggga
      361 gcgatcccgc ccgcggccgt caggtaaatt tttccgtcgg ccgtgacttc acaggcttca
      421 ataatggcga tgtccacgtt gcccatgaac ccgtagcgca cttcctgggc catctgggac
      481 aaatggatgt cattataggc gatttccccg ttattcaccg ccttgcggaa atcaacgttg
      541 gtggtgtagg gagcgcggta gcggattgcc ttggcccggg aaaggatccc gtcacaggaa
      601 tcgccagtgg aagcaccggt gaagacactc acctggaaag ggttcccctt ctcatgttcc
      661 gcttctgcga tttttgccag cgcagccgta acggccttgg ccgttccggc gggcgtgaat
      721 ccgctcagcc caatatggtg gccatgcttg atcaggcttg ctgcttcttc tggtgtcagt
      781 accttgtaac tcatgtcagt gatttttgtt ggatgaaaaa agaaagaaac cccgtgtaaa
      841 aacggagcgg caggctcatt cttcgaggag cctgttgatc aggtgcgccg gaatggtgcg
      901 gacggtgccg gccggaaggg atagcacagg attgccctga tcgtcaatcc ctgtaaaaat
      961 gccccggacg gtttcgtcat ccgtaagaat ggaaaccggc ctttctccgg cccatgcccg
     1021 ggacagaccc tcctgaaggg cggggacgcc gccgtgggaa aagacggaaa gaacctggtt
     1081 caaatgactg cccagggaag ccatgacggc gtgcagggaa gggcagtccc gaacaagatc
     1141 cgccagcctt gccggagggt ccttgatttc cccggcgatg gaggatacat cattgaaaac
     1201 attgatgccg atgccgatga cggccatgtt ctccgccggg cgttccacca gtattcccgc
     1261 cagtttggat ttgcccgcca gcaggtcgtt cggccagcgc aggcgcacgg aatccagccc
     1321 catgccgcgg agcatgttga acagggccag ccccgcaacc aggggcaaat gtccccagtg
     1381 cacgccggcc gctgtatcct caggagcgga cagggggacg gtgaaggatg cccacaggcc
     1441 gccttcctcc cctatccatt tgcggttgaa cctgccgcgg cctcctgtct gaacgcggca
     1501 gacggcaata tgccccgggg ggagggctcg ggccagatca ttggtggaaa aagcctcggc
     1561 ccattcatga taagtccagt cgccgcatgg tccttcctcc tccggagggg tggaaaatga
     1621 ggctgcggaa ttcatgggac gggccgaggc cgggagtaat tttcaattct ggttatttcg
     1681 ccagagtggc gatgtagtaa cccgcgatga ctgcggtgcc gatcacgcct gcaacgttcg
     1741 gacccatggc gtgcatgagc aggtagttgg aggggtcgta cttctggccc acattgtggg
     1801 agacgcgggc tgccatcggg acggcggaga cgcctgcgga cccgatcagg gggttgaccg
     1861 gattcttgcg ccagatgagg ttcataatct tggcggcaat cactccgccc gctgttgcca
     1921 cgccgaaggc gacgataccc agaaggatga tcagcagcgt ttccgactgc aggaagcggt
     1981 cgccctgcat cgtgagcccc acggaggttc ccaggaagat ggtgacgatg ttgatgattt
     2041 cattctggga ggcctgaacc aggcgttccg tcaccttgca ttcgcgcagg aagttgccaa
     2101 gcatcagcat gccgatcagc ggggatgcgt ccggaatcag caaaatggtg acaatggtta
     2161 ccagcacggc gaaaaacagt ttttcgctct tgctgaccgt tcgcagggat ttcatgcgga
     2221 tgaggcgttc cttcttggtc gtcagcaacg ccatgatggg cggctgtatc agcggaacca
     2281 gggacatgta cgtgtatgcg gccacggcta cggcgcccag caggtgcggg gccagcttca
     2341 tcgtcaggaa gatggaggtg gggccgtcag cgccgccaat gatgccgatg gacgcggatt
     2401 cattcggatt gaaccccatg aacagggcca tgaagaacgt ggcggcgacg cccacctggg
     2461 ccgctgcgcc cagaagcagg gtgcgcggag cggcaagcag cgggccgaag tccgtcaggg
     2521 ctcctacgcc cagaaagatg atgggcggga aaatttcagc cttgatgccc agcccgatcc
     2581 agtcatacag gcccccgtgg atggaggtga ccagcagttc ctgggtatgc tggcctacgg
     2641 ccacgtattt gcctgcggcg gaaaccacca ggggctccag cttctgggtt ttcagatcca
     2701 ccggaatccg gttgccgctg gtgtcaaagt aattgacgcc ctgctggcgg acgacggtgg
     2761 tcccttctcc gttcgtgagc aggaagaccg gttcattgcc gttgtcttcc gtcacgttct
     2821 ggaaatcctt caggttgggg tccaccgtca ggctgtcgtc gcccaggcgg gccgcgtcag
     2881 cttttgcttt ttcctgggat tcccgcacgg acttgatctt ggaaacggtc agctggctgc
     2941 cggggtaaac ctgcatgggc tgctgcatga tttcctgata ctgggcacgg gattcaggcg
     3001 tggtcagatt ggccggcacc ttggagggag cctgctgaag cggagccacg tgaacgcgca
     3061 ggtaccccac gttgttcagg ggcgtagcgg ttacttcccc ctgttcattg gaggagatga
     3121 cctgctggtt cagctgggtg atgagcattc cgttatccgg aatgttggca atgagggcgc
     3181 cgaaagcaat gggaaccatc aggagcggtt cgtattgctt ggctacaccc agatacagga
     3241 ggagaatggc aatgccccac atccctacca tctgccatga tagggagaag acgcccatcc
     3301 cctgtaaaaa ggtgattaat gaatcaaaaa gactcatagg gaatattttt gtagcggttt
     3361 attatttgag ccggagcccg ggactgttgg cgtttccggg ctgccgccgc cgggattaac
     3421 caattttggc cagtgcctgt ccttcctgaa ccgtgtcgcc cgggttcacg cagaaagcgg
     3481 tgagcgtgcc ggaggcggg
//
LOCUS       DAWEMX010000191         3499 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9210_length_3499_cov_14.981417, whole genome shotgun sequence.
ACCESSION   DAWEMX010000191 DAWEMX010000000
VERSION     DAWEMX010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9210_length_3499_cov_14.981417"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    654..663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          736 a          844 c          988 g          921 t
ORIGIN      
        1 gtttaccgtt gttccggaca ggttggaata ggttcgggaa ttgtgttcct tcatgccgga
       61 aaacatgatg gcctgttcca gaacaggttg tccacagatg accgcattca tctgtttccg
      121 gccgtgtgaa cagtgcggga acggtttctc taaagagtag ttgcatcctg atattttttg
      181 gattatatca tttttcatga aatggctttt gattttcctg ataagtccct gcatggcttt
      241 tgctgaaagt atcaggggag tcgtgattaa cgtgattgac ggcgacacgg tcatccttct
      301 ggaaaggacg cctgaacaaa tgcgtacgca ccggatacgg cttgagggaa ttgatacgcc
      361 ggaaaggggg caggacgggt atgaaggcgc caaggaatac ctggaaaagc tgatttgggg
      421 agagacggtt acagtccggt ataccgagaa tgacaggtac ggccgtattt taggggaaat
      481 atggcatgga aaaatgttcg tcaacgaaga gatggtgaag gaaggatggg cgtggattta
      541 taaaaatttt tccgccagcc ggaagctcgt ttctcttgaa agggatgcca gaaaggccca
      601 aaaaggcatc tggtctgacc cgaatcccat atctccgtgg gaatggaaag cagnnnnnnn
      661 nnnaaaaata ctggggagat ttcttattaa atcgttttcc gggaaaatgc cccgttcgtg
      721 atgctgctgg acaggggatt ttcaataagc aggaaaaacg gatgctttta acggcgggcg
      781 tggttcagaa gcatttccgt tgtggcctgc ccgcctgctc cggttcaaag atggcgggca
      841 gcggagtgtt tgccctggag ccttgccagg gccatgtttc agggttggcg cggtaaggca
      901 ggagataatc cacagctttt tgaatggaag ccccggcggg tgttttccgg taacggtagt
      961 tcttgtcgcc catgcgttct gcaagatgca ccagatatcc ccatgcctga agagtgtaaa
     1021 cgctgtattg ccagggttcc gcccgtttga gttcttcggg ctgggatccg tcgggagcga
     1081 tgtgtcggtc catgttggcg aaggcttctg ccagccattg ggccgcttca tggctgcggc
     1141 ccaggaagga tgccaaatgg gcgcattgcg ccacataaaa cagataatgg ttgttgctgc
     1201 gcgtgtgtgc atgcctgccc tgcctgctgg tagtcagcca tgtgtaatac tggttcagcc
     1261 atgcgtccac ctgcttccat tcctctgccg ttagggcatt ggagcatttc aacgccgtca
     1321 aagcttccag gaattcgggc atgcggtaaa ggtcgatgat gcctcctccg cctccttccg
     1381 tccggtgtcc ggggcgaatt tgggcatatt tcagatgggg ctgcatgcgg gttgccggag
     1441 caatgaacca ggctttcagc cattgcccgg ctctggaggc ggcctgcgga tccctgtcga
     1501 gttcatactg ccgtcccagg ctgcagaccg tgctgatgaa agcggccatc ctgccgctgt
     1561 cgtccatgct gcggagccgt ttgttctttt tgccgtcaat gggtttgtaa ggaccgtccg
     1621 gattggcggg gtccggccag taataaaggg catagctgac atattcattg gcctgcgctt
     1681 tttctccgaa caggcttttt ttctctccaa gggtgatgac gggtgcgttc agaggactgc
     1741 catgcaccat ggaaaagaga aacgccgtga aagggaaaaa gatgaaccaa aacctccaaa
     1801 atgccattac cctattcaag caggaacctt ttgccgggac aaccgggaaa tggagcaatg
     1861 ccctgatttc cttgcagagc cgtttccggc cgctttcccg gtcatccggg ggtgtggcgc
     1921 tgcggaaagg gattccggaa agtcagtccg gcccttttcc cggattgcgg gtttccgcat
     1981 caggccagat gcatgccgcc ggtaacgcca aagacctccg cagtaatgaa gctggattcc
     2041 tgggaggcga gaaagacgta tagtccggag agttccacag gttgccctgc ccttttgaga
     2101 ggcgttttcc gtccgaattc cggcaggtcc ttctgcagct ggcctccggt tacttgcagg
     2161 gctgtccaga tggggccggg agccaccacg ttgacccgga tgcctttggg ggcaacctgc
     2221 ttggccaggg agcggctgaa cgcaatgatg gccgcttttg tggaagcata gtccaccagg
     2281 ttcttgccgg gctggtaggc ctgaatggag gagcaggtaa tgatgctcgt ccccggcttg
     2341 agatgcggaa gggcttcctt ggcaatccag aacagggaga acacgtttac ttcaaatgtt
     2401 ttggttaatt gttccgtggt aatgtcccgg atatcttcca ccgccacttg tttaccggct
     2461 accagggcca tgatgtccag tccgccaagt ttttccaagg catccctgac gagtttttta
     2521 caaaagactt catcgctgag gtcccccggc agcaggacgg ccttccggcc ctcttttctg
     2581 atgagttccg ccacttcttc ggcatcgctt tgttcttcag gaaggtagtt caaggctacg
     2641 tcggctcctt cgcgtgcgta ggcaatagcc gccgcccggc ctattcccga gtctccccct
     2701 gtgaccaggg cttttcgtcc acggagcctg ttgcagccat ggtagctttg ttccccggaa
     2761 tccggcacgg gcttcattcc ggattgcagt ccgggggctt tctgctgctg ctttttatat
     2821 ctttcctgtt ggaattcacc gcgcgggtcc tgtatttcgt tagtgccttc tgtgctcatt
     2881 ttttcgtcag tcatattttg atgcggagtt gacggaaggg gaaaagcgct gccatgcggg
     2941 aaatagccgg cagcattccc ttttccggca ttcttcattc ctatcgtgat gaccaggctt
     3001 cggacggcct gttcagggaa agacatgttt ttatttcaat atgccattga tatttattta
     3061 tataggaatt ttctaaatgt tgtttttatt ggccggagaa agttatttga ttttcagatt
     3121 tcctgaattt ccccaatcca ctttagggga aaaacatcct tgggatgatt gatgatttta
     3181 gaataattta atttcttctt ctgttttacc accgtttttg atgccgtccg taagtgaaat
     3241 atccggctgt atccgccagg agcgtcggaa gggtgggaat gtctttcatt tctacggggg
     3301 tgccaaccga aggcgccgca gaaagccggt atctggatgg tgtcagcccg tccccaaacg
     3361 gctaagaccg gggaacgaag ggaaaagata tttctttcct tcctgtttac aggcggaaaa
     3421 aaagaaatga aagaaaacag aatcagcttt cttcctgttc cagtttcgct ttcctggcca
     3481 ggattttttt tcatgacgg
//
LOCUS       DAWEMX010000192         3494 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9236_length_3494_cov_7.722012, whole genome shotgun sequence.
ACCESSION   DAWEMX010000192 DAWEMX010000000
VERSION     DAWEMX010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9236_length_3494_cov_7.722012"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1135..1144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1783..1792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          693 a          881 c         1156 g          744 t
ORIGIN      
        1 ggtatatgcg gtcagcatgc cggaacccag gtaaaggagg aaaaacaggg ttacatcctg
       61 taccagatta atttccccgt agtaataccg gatgagaaag aagggcaggg agataaaggc
      121 aatcagcagg gattgggcca tgaaggatat ccattttccc agcacgatgc ggcctgcgct
      181 ctgcctggtg agtagcagca gttcgttgtt tcgggtgagc agttcgttcc tgatggaggt
      241 ggaggccctc agcggcatga tgaagagcag ggcgacgacc atggtaaccc agaagatgat
      301 ctggcatccg tcggggctga tgaagggctc cccgtcaaga ggattgatga tgaagctgaa
      361 caggaagaaa aaggagagca gggagggcac tatgctgacc aggaggatga atgacggcgt
      421 tctgagaccc tggcgcaatt ccttgaccag agcggagggc atccatatgg ggaaatcaag
      481 agagcgggag gtattcatga ggcggggatg gatgggcttt cctgggaaga ggcgggattc
      541 tgccgcgggg ccggttcccc gctgccgtgg ataaggatgt ccacaaaagc ttcttccaga
      601 cggcgttcgc tgcggtggaa atcaattacg gggaggtcga ggcagagctg gcggagttcc
      661 gcagccacag cttccggttc gcaggagacg aaggcggcca ctactttctg ttgctgtaca
      721 tggcgtacgt tccatccccg gcggagggcc agccattcct ccagtgaggc ggtgtcgtct
      781 ccggcggtgc ggatttcaaa ggcatatccg gattccgtct ggcggtgcag caggtcttcc
      841 atattgccgt catggatgac ggttcctgca ttcatgaaga tgagggcgtc gcacatttcc
      901 cccagttccg agagaatatg ggagctgatg agcagcgttt ttccgcgcgc tttgagcaga
      961 tgcaccagat ttttgaattc aaggcgcgct ttggggtcca atcctgccgc cggttcgtcc
     1021 agaatgagga attgcgcgtc gctgatcagc gtgcgcgcca ggcagagccg ctgcgtctgc
     1081 ccttttgaga gtttatcgat catctggttc tggaggccgg ccagttccgt gaagnnnnnn
     1141 nnnntgaagt ccatcacttc gtccacggcg tccagtaaac gttccctgcc catgccgcag
     1201 gccctggcga aaaaatccag atattcccgg acgctggtgt ttttataggg gtggaaatag
     1261 tccggcatcc atccgatgcg ggaacgtgcc agttcgggat gatccacaac atcggccccg
     1321 ttgagcctga tgtggccgga gtcaggcatt tccagcgtgg ccagcatgcg catgagggtg
     1381 gttttgccgg ctccgttgga gccaatcagg ccgacgacct ggccctggcg gatgacgaag
     1441 gaggcattgt tgacggcttt cagcggaccg aatgatttgg acagtccgga aacttccagc
     1501 agtaggggcg tactcatggt tattcgtgcg gcaggggagt gacggggccg gagacggtga
     1561 cggaggtttt ttcccagtta atggaaggca gggtggggat ggggcccagt tccccgcccg
     1621 ccggttccat ggatgcccgg aagtgcagcg ggggaggtcc gtccggttct tcttccggca
     1681 ggaacgggga tgcctgcatt ttacggccgg gaggcagttg ttcaagagtc cagtattttc
     1741 cggaggcgtc ccggtaggtg agtccgttca gggttccggg aannnnnnnn nngggcgcgt
     1801 ctccagcaga gtgagcgccg cccgtgtgga aaccggcatg atgatccggt gctggagcgt
     1861 ggagcggctg gtgaaccatt ttcccccgca ttcttcccct tgccgcgccg cgccttctat
     1921 gggtttttcc ttaatggagc cgcgcgcctt gctcatgcgt gctccggtga tgccggcgtt
     1981 ttccggcaat tgaaatgtat tgttcagcag gacggaggtc ttgcaaatct ggttttggga
     2041 gatcagggcg gagtggtcct gcggattaac ctggataaga actttccggc tgcccgtccc
     2101 tcccgtgccg tctccggcca gaatcaggag cagcagaaga agggaaaatc ccacggaaat
     2161 ggccgggatc agcaggaaca gcctttgcct tttgcctgcg ggagcccaca ggaacaggct
     2221 cagcgggccg acaagcacgg caaagatgat cagccccagc cccagcgtgg tggatggggc
     2281 tgagaaataa tagggagcgg cggaggtaat ctccgggtaa cagtttccct tttcgtggag
     2341 gttgatgaat tccttcagtt tcagcttttt ttcttcttcc ggcaggccgt ccagggacgg
     2401 aacgtgcaga atgcgcccgt ttcccagcaa ccgggtggaa acggaaagcc ccctgtcacc
     2461 gatgagccac aggtttcccc ctcccgtcac ccattggcgg atggctttgc ggtgggcttc
     2521 gtcaaaatgg gtacggtagg ttttttccgg gatgatgagg caggtctgcc cctcgtacat
     2581 ccggtagtcg gctggccatt tcatcaggtc gcaggaggaa acggccgcgt tataggaaga
     2641 ttcggaaagc aggtgccagt ccgccggagc gccttcctgg ataaccgcga ccattttcct
     2701 gtgctccgta tccgtgggaa tgggggaagc tccttcgtgg ttttccttca cttcgtagac
     2761 gagattgtag gagaggagag ggtaaacgac tgcatgaacg ctttcccggg ccggaatgaa
     2821 gaacggataa gtatggctgg ttcccctgtt tccgttccac ccttcatgga attgaagagt
     2881 ggcttttttg tccaagccgg tggtgttttc cagcttcacc cggagaggga cgcgggcgcc
     2941 gttcgcgctg tagtcaaatg ctttctccac ggaaacggcg atggggggag cggcggccgg
     3001 ttttgcgtgg agcgggagaa tattgaatgc cgaaagaagg aagaggctga agaacagcca
     3061 tgaacgggag agagggcggc gcatggggga tgggctgagg aggtgtggtc aatgaatttt
     3121 tgcggcggcc agggaatccg gcaggggctt gttctgggca gggatttccc tgaccaggag
     3181 gttgatgact gcggcgggtg tctgtccgtc cagacgggcc gtgtggttga gcaggatgcg
     3241 gtgctccagc acgggatgaa taacggcctg cacatcctca aaggagcaga aagcgcgccc
     3301 gtggcgcaaa gctcgggctt tggcggcagc cgccaggccc agggccgccc gggggctggc
     3361 cccgtattta acgcctccgg aggcttccga ttctccggga tgcgtagcgt tgaccagcct
     3421 gcctatatag tccgccacca cttcgggaat ggctacattc cgggtcattt gcatgagttc
     3481 ctgcaattcc tgcg
//
LOCUS       DAWEMX010000193         3459 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9339_length_3459_cov_9.556992, whole genome shotgun sequence.
ACCESSION   DAWEMX010000193 DAWEMX010000000
VERSION     DAWEMX010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9339_length_3459_cov_9.556992"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          738 a          973 c          938 g          810 t
ORIGIN      
        1 gtgcaggctg ccgatggaaa ccgggccaag acgggcggga acggggggga gatagccgcc
       61 ttccgctccc ttccagcgca attccctggc tttctccagg ggcaggaggt cccgggcggg
      121 tttgttttca tgattgttgc gcaattcctc ctgacgggcc ttcacggcgg caatgtagga
      181 atcctttttt tctcccacca gggaggcggc tgcgggaacc acctgggagg catcctccgt
      241 atgcaccacg gctccttgat aatggggggc tattttcagg gccgtatgca gggctgacgt
      301 cgtagctccc cccaccatca ggggaatggt catcccccgg cgttccatct ccgcggctac
      361 atgggacatc tcttccaggg aaggggtgat cagcccggaa agcatcacca ggtccgcctg
      421 ttccgtttcc gcccggtcca gaatggtatc gcaatggacc atgacgccca ggtcaatcat
      481 ttcaaaacca ttgcagccca acaccacgcc gacgatattt ttgccgatgt catgcacgtc
      541 ccccttcacg gtggcgatca cggcttttcc tgccttggcg gctcctttgc tgtccgcttc
      601 tatatagggg gtgagccagg ctacagcctg tttcatgacg cgggcgcttt tcaccacctg
      661 cggcaggaac attttgccgt ccccgaacag ttgcccgaca actttcatgc cggccatcag
      721 agggccttca atcacgttca gcggagaccc cagctcctga aaagcctcct ccgtatccgc
      781 gtcaacaaat tcggtaatgc ccttgataag agaatattcc agacgtttgg caacgtcctg
      841 ctccctccat gcctgtacgg tttttttctc ctttccgtct ccggtttttt ccgcagccag
      901 tttttccgcg taatccgtca ggcgttccgt ggcgtccgtc ctcctgttca gcagaacgtc
      961 ttcaatcagc tccagcctgt ccttgggaat attgtcgtac acctccagca tcccggcatt
     1021 cacgatgcac atgtccagcc cctgctgcgt agcgtggtac aggaaagcgg aatgcatggc
     1081 ttcccgcacc gggttgttgc cgcggaaagc aaaggaaaca ttggaaatgc cgccggaaat
     1141 gtgggcatgc ggcaggttcc gggagatcca gcctgcggcc ttgaagaaat caagagcgta
     1201 atcggcgtgt tccgcaattc cggtgcctac ggtcaggacg ttcgggtcaa aaatgatgtc
     1261 ttccggagga aactgcaccc ggtttaccag caagtcatag gcccgtctgg agatgcggac
     1321 gcggtcctcg taattggcgg cctgcccctg ttcgtcaaac gccatgacca cgaccgccgc
     1381 gccgtacttt ttaatcagcg ccgctttctt caggaattct tcttccccct ccttcagaga
     1441 aatggaattc acgattccct tgccctgcat gcattggagc cccgcttcca gcacctccca
     1501 cttggaggaa tccaccatga aaggaacgcg ggcgatatcc ggctctgcgg aaacaaggtt
     1561 caggaagcgg accatggcct gcggcccgtc gatcaggccg tcgtcaaaac agaaatccaa
     1621 taccagcgcg ccgttttcca cctgctgccg ggcaatggat accgcttcct cgtaatttcc
     1681 ttcacgaatc aggcgggcga atttggggga acccgccaca ttgcagcgtt ccccgacaaa
     1741 gagggtgttt ttttcccgcg tatggttgta cgcttcatat ccggacaggc gaagcgcggg
     1801 cgtttgggga gccgaaacgc gcggaggcat tccttccacg gcagccgcaa tggcgccgat
     1861 atgctccgga gtggttccgc agcagccgcc cacgatgttc agcaggccct gttccgcgta
     1921 ttccttcatg aaccgggcca tatccgccgg cagaaggtcg tagccggtag ggctgagcgg
     1981 gttgggaagc ccggcattcg cataaatgga catgtggcaa tccgccagtc cggacagttc
     2041 ttccgcaaac gggcgcatca ggtccggccc cagggcgcaa ttgatgccta cggagaaggg
     2101 gcgaacgtgg cggatggagt tccagaatgc ttcaatcgtc tggccggaca gcgtgcgtcc
     2161 ggctttgtcc gtcagcgtta cggatatcat gaccggaaca gagctttccg gttgttcctc
     2221 aaaaatttct tcaatggcga acagggctgc cttggcgttc agcgtgtcga aaatcgtctc
     2281 caccagcagc atgtccaccc ccccttccag cagggccagc acctgatccc ggtacgcctg
     2341 cctgagctgg cggaaattca cggcacggaa accgggatca ttcacgtcgg gggacaggga
     2401 acaggtgacg ggcatggggc cgatggatcc ggccaccagg cggggcttgc cgtctgcagc
     2461 ttccgcttcg tcgcatgcct gccgggccag tgcggctgcc gccaaattca attcccggac
     2521 aagggccttc aattcggggg cgtccaccac ctcctggaaa tactgctggt ccttgtggct
     2581 gccttcctcc ggacggtgcc agaaataatc atgctggccg atgctggtag ctccgaaggt
     2641 gcaggtttcc agaatatccg tccgtcccgt gtccagaaag cggcggtgaa tatcaagaat
     2701 gatatccggc ctggtcagaa ccagaaggtc gttattattc tttaaatcgt tgggatagcg
     2761 tgccgcatcc gcaaaacgtt ctccgcggta atcttcctct cccagcctga atttttggat
     2821 attggttccc atggcgccat ccaggatgac aaccctttta cgtaactggt ctttcaggta
     2881 atcaaggcgt tctgttctgg atgctctgct catatggcgc ggaaaatcta ccgtccgtcc
     2941 gtcattggaa caaggacaat tgtcgtcacg ttttttccgt attgctccgg ggaaatggaa
     3001 ggaatctgtt ttcaagggaa tggaaaaggt atgggaatga tttacggaaa gtaaaaacgc
     3061 ctgtgaaaat tttatgcttc attccggatt ttctggaagg atgggaaatt tccatttttt
     3121 tattccgttt ccgtatgtcc ctttcctgtt tcacgctgtt cccgggaaaa agaatgcggg
     3181 gactggagaa aaaggaagaa gaattgtgaa taaaatgata ataatatgtg tataaccgga
     3241 atgactattt atgtttcaat agcggaaaat tcattcatgc cgaacatagg gaagggacga
     3301 aagcgtgctt tttcagtaac attatttact ggcaggcatt ctgaatatta cccgataaag
     3361 aaacattttc ttttccagaa atgaagagcg gatttctaat ccgaggtcaa gttcccagtt
     3421 tgaaaggaaa ggctttgaga aaaagtcccg atgcttcac
//
LOCUS       DAWEMX010000194         3367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9602_length_3367_cov_8.614130, whole genome shotgun sequence.
ACCESSION   DAWEMX010000194 DAWEMX010000000
VERSION     DAWEMX010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9602_length_3367_cov_8.614130"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          700 a          884 c          912 g          871 t
ORIGIN      
        1 tatggatatc gccatagaac cctatggtga taaaaagata tgcgtatact atgaatagaa
       61 tttccttaaa ttcccaaaac aacaaacaac aacaaataat atgagaacaa caatcaccgc
      121 atttctggcc gcattgattc ttcccggtct ttccatggcc gccgcagatg aagccaaaac
      181 gatcaccgcc acgcacacgc tgggcacggt cgagcttccg gtcaatccga aacgtgtcgc
      241 cgtgctggac tacggttcgc ttgagaccat cggtgaactg ggcgtcactc ccgcgctggc
      301 tttacccaaa aaattccttc ctccttacct ctccaaattc tccggagaac aatatacgga
      361 tcttggaacg gttaaggaat tcaatattga aaccatcaat gcgttcaagc cggacttgat
      421 tattatttca ggacgtcagc aggattatta tcaaaaactt tcctccatcg cccccgttta
      481 cctggtcgat actctggcca aggatcagct gggggaagcc aagaaaaaca tcaggcttct
      541 gggtgatgtg ttcggcgtgc ccgaaaaggc agccgaagcc gtcaaaaaca ttgatgaagc
      601 cgtaacccgt acgaaggaca aggcccaggc cagcggcaag aaagctctcg ttctccttac
      661 gaacgacggc aaggtaagcg cttacggttc gggttcccgc tttgggttgg tacacgatgc
      721 cctgggagtg gcccaggctg ataaaaacat caaggtgggg gttcatgggc agcaggtgac
      781 ttatgaatac attgccatgc tcaatcccga tatcatcttt gcggtggacc gttcggtagc
      841 tatcggccgc tcggccaaaa atccgaatgt cctcaacaat gtgcttgtga ataaaacgaa
      901 ggcggctaaa aacggcgcca tcatcctgct tgacccgcag gtatggtatc tttccggcgg
      961 cggcatcatt tctctgaaca agatgatctc tgaagtggaa gctgccttta cggacaaaaa
     1021 ataattattc cgtctttatt catcacgaag caggccgcac gaccctatgc ttccgtttcc
     1081 cggctggagc ggcagaggcg tgcggccctt tttcattcag tatctgctga tgaagaaaat
     1141 tttgcagtgg atggcatctc cccgtctcgc gtgcgtgctg atggcttacg ccgtgctgct
     1201 catttttctc ggcactcttg aggcgcggtc tgtcggtgtt tccgctgtac aggccaggta
     1261 ttttgaaagc tggggctgcc tttccttcgg gatgattcct ctgatcggcg gcgccggcgt
     1321 aggggcatta gccgtactga atatttcggc gtctgttttc cggcgtgcgc gcttcacact
     1381 gcgcggcgtg ggcttgatcc tcacgcatgc ctttctggta gtactgattt tggcgggggc
     1441 gttgcagtat tttctgcgta cggagggaat actcgttctg gatgcaggtg aggtctcaca
     1501 cgaaatactg gcaggcgaag ggaatggaag aaaaaatatc ccccttggtt tttcagtaaa
     1561 gctgaaaaaa tttgatgccc ggacatggac gggaagcgat atccccagca gcttttcaag
     1621 cgaggtttgc tttatgcgcg gcggggaaag cacggaaacg gttattcgca tgaatgctcc
     1681 ggtctccttt ggcggctgga ttttttacca gagcagttac gccaatgggg gccgcacgag
     1741 tgtaatcaca gccgtacgca atccggtgcg cttttttccg tggatatcgg tgcccggcgt
     1801 atttgcgggt atgttgctga tttttctccc aaccctgcgt gcccgaaaaa aacatgcagt
     1861 gtaaaattac tttatttacg gcgtttttcg ttgctcttgc gattgcgtta tcgcctctgt
     1921 ttcagaggat tggtagttct ttttcctcat ttgacggcat agggacgccc tcacccggcg
     1981 cgctgccatg cctgaaggct ggacgagtga tgccggtttc aagcgcagcg gcggattccc
     2041 ttaaattggc aagcggccgc acgtcggtga atcttggagg ctctcggacc gggccggtga
     2101 agtggttgct ggcattaaac gcctgcccgg caaaaatggc cggcgaacct tttctgcgtt
     2161 cggataacag ggatatggtc gaattccttg gcggacgagg ccgttatttt tcctatgccg
     2221 atgcagttga gaaggctggc ctgctgcgcg gctctctgcg gagaaattct cctcaggccc
     2281 atgccgcgga agccgtactg ggcgcactcg ccgtttatga gcaggccggc gttgcgctcg
     2341 ctctccgcct ggaggggaca tcgggctgtt tggggacgct tgatgcatgg catgaagcta
     2401 tggccgaagc ctcagcagag cttgaagccg ctgaacgtga aaaacgccat ccctcgcttg
     2461 accggctcta ccgtgccaat cgctattttc aattcctcca ggctcttgtg caggtagaag
     2521 aagcggatcc gaatgtggcc ctgcgagtaa tccctcatga gggaagctac ctgaccccgg
     2581 ctgctgctct gctcgaaaaa aatctcagtc ccgccgggtg gaccgccctg aaagcctatg
     2641 ccgcagcctg cgatgcctat gctgccggag atgaatctac tgcggcagag actctgcgtc
     2701 aactttctgg ggaattggag tcaatactgc ccatggattc tttcaaactg catttcgagc
     2761 attgcgtcaa tgctatcgaa cccttcttgg gaggcttcct tctctacggc atgtcgttgg
     2821 tttgtttcgt ggtctctctg tttctcaagc ggcggaagac attgctgcat tttgcgacgg
     2881 tcttcctgtg tatggcggta ttatggcatt tattcggcat tgcggcccgc atgtacattc
     2941 aatcgcgccc cccggtaacc aatttgtatt cctcggtcgt ctttgcgggc gcggtagcct
     3001 cattattcgg cgggacgctt tatatcctcc gccgtcagct tcctgttgcc gcggtcgcct
     3061 gcgcttctgg tcttctctca cttctggtag ccatgaattt gccctacagc ggcgatacca
     3121 tgggcatgat gcgtgccgtt ctcaattcga atttctggct caccatgcac gtaatgacca
     3181 tcatgatagg ctacggaagt gttttttttg ccgggttttt agcctcatat catctggtgg
     3241 cccgtttctt cggacagggt gaaaaggggc tgaaagccgg ggcctgcgga gtctatcgca
     3301 tccttctcgt ggcgctcttc ttctgttttc tggggacaat gctcggcggc atctgggccg
     3361 acatgag
//
LOCUS       DAWEMX010000195         3360 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9616_length_3360_cov_7.818457, whole genome shotgun sequence.
ACCESSION   DAWEMX010000195 DAWEMX010000000
VERSION     DAWEMX010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_3360_cov_7.818457"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          750 a          979 c          983 g          648 t
ORIGIN      
        1 ccttgccttt gacccggcgc ctgtgtagtt tgtttaaccc ggcgcccgcc tgtgtcgggt
       61 ttaaaagcct ttcccccgtc cgccagcgtt tcatccagga aacgctggcg gacttccgcg
      121 agagcggggc ggacctgccg gacgacgtga agccggaata tgtggagata gaagcccagc
      181 tctccctgaa gaccaagaaa ttcgcggaaa acgtgctgga ctccaccaac gcctgggaac
      241 tcattgtgga agatgaagcg gaactttccg ggctgccgga ttccgcgaag gaagccgccc
      301 gcctggatgc cctggccaac ggccacggca cggaagaagc cccccgctgg cgcttcaccc
      361 agaaattcac ctccctccag cctgtcatgc agtttgcgga ctcggacagc ctgcgccgcc
      421 gcatgtggga gggctcctgt tccatcggga agggcggaga atacgataat gaagccctta
      481 tcgctgaaat cctggaactg agggacagga aagcccattt gctggggtac ggctgctttg
      541 cggattacgc cacttcccgc cgcatggccg ggagcggagc caacgccctg aaattcatca
      601 acgacctgca tgacagggtg aagccctctt tcctgaagga catggaagcc gttcgcaggt
      661 acaaggagga aaaaacagga aaacccgtgg aaaagctctc cccgtgggaa accggatact
      721 ggtctgaaaa acgccgccgc gaattgtacg cttttgacga ggaagacctg cgcccgtatt
      781 actccgtgga aaaagttatg gaagggctct tttccatcta ctccggcctg tacggcatca
      841 cggtcacgcc gcgtcccacg gtggcgttca agcccggtga atccggggaa gcgccggaag
      901 gcgcggtgga ggtgtggcat ccggacgtcc tgttctatga attgcatgat gcggaaagcg
      961 gggaacacct gggttccttt tatgcggact ggcatccgag ggactccaag cgcgccggag
     1021 cgtggatgaa ctacctgagc gtaggggaac ctccgcacgg cggaaaaccc cgcgttcccc
     1081 atctgggtct catggtcggc aacatgacca agcccgtagg ggacaagccc gcgctgctgt
     1141 cccaccggga ggtggaaacc atcttccatg aattcggcca cctgctgcac cagctccttt
     1201 ccgatgtgga agtgaagtcc ctgtcgggca ccaacgttgc ctgggacttt gtggaactgc
     1261 cctcccagat taatgaaaac tggtgttggg agcgtgaatc cgtggacctc ttcgccgccc
     1321 actatgaaac gggtgaaaaa ataccggacg aactgttctc caaaatgcgc gccgcccgca
     1381 attatatgag cggcacggat ttcatgcgcc agctctgctt tggcaagctg gatctggagc
     1441 ttcacgtaaa ctggcctcag tacaagggtg ttccgctgga agaaacggat gaacgcattc
     1501 tggcggatta ccgggtgccg atgacggacc gcggcccttc cgtggcgcgc cgcctgaccc
     1561 acatcttcgc ggatcccacg ggttatgctt ccggttatta ctcctacaaa tgggcggagg
     1621 tgctggaagc ggacgctttc agccgcttcc tgaaagaagg agtgctgaat ccccgaaccg
     1681 ggcgcgactt ccgccgctgc atcctcagca agggcaacag caagcctgcc gctgaactct
     1741 accgcgactt catgggccgt gatccggacg cggaagcgct gcttgtcaaa tccggcgttc
     1801 tttaacctct ctacccatat tatctgcaca atccttatgc ctgaacaaaa gagactctcc
     1861 ctcgccggca tcgggtgcgg ttcccgtacg cgcacctaca tgaagctggc catggaacaa
     1921 aaaaaccgct accgcattgc cgccgcggcg gatcccgtca aacagcgcac ggaagccgtt
     1981 cgtgactttg ccccggagga ggaacgggat tccgtccgcc tgttcaagga tgccgcttcc
     2041 ctgctggcgg aaccgcgcct ggcggacgtg gccatcatcg gcacgcagga cgactaccat
     2101 tacgctccgt gcaggaaggc catggagctg ggctatcatg tcctgctgga aaagcccatc
     2161 gccaagacgc tgaaagaagc gctggagctg agggatacgg cgcgcctgct gaagcgccgc
     2221 gtggccgtgt gccacgtgct gcgctacacc cagttttacc ggaccctgaa aaaaatcatc
     2281 gacagcgggg aaatcgggga agtcatcacc ttcaatgcca atgaaggcgt gggggcgtgg
     2341 cactttgccc actccttcgt gcgcggacac tggggcaaca gcaaaacctc cactcccatg
     2401 atcgttgcca aatgctgcca tgacatggat atcctctact ggctcatggg ccggcgtaaa
     2461 tgcctttccg tcgccagctt cggggagctt accttcttta cgaaaaagac gctctcctca
     2521 ccccggccgg aacgctgcac ggactggacc accccggtgg gggaagaccc ctgggacgcc
     2581 cgcaaatacg ccacggacga cgaatgcaaa cgctggctgg gcatggtata tgaccgggcg
     2641 caggaagcta ccgcggagga aatctgcgaa tggctgaaaa cctccccgtg gggcagggac
     2701 tacctccagt gcgacaacga ccagccggac catcaggttt ctatcatgcg ctttgaggga
     2761 ggcttgacgg gcacgttcac gatgacggct ttcgaacagg ggcgccacat tgaagtgtat
     2821 ggcaccaaag gcaaaatccg agccggagtc ttctacaagg aaaacggccc cggtgaaata
     2881 accgtgacgc cccactttgg cggaaaaacc cgcgtggtgg agctggagga actggccgga
     2941 ggctaccagg ggcacggcgg cggcgactgg ggattgattg aggctctgta tgatgacatg
     3001 ctgacggtgc cctctccgga ggatatgacc acttccattg aggaatccgc ccattcccat
     3061 gtgatggcct ttgccacgga acacgcccgg ctgagcgggc aggtggtgga tgttgctgac
     3121 ttcgaacgca aggtcgcgga aggcatcctg aattattgat gcggttttcc tccttgaggg
     3181 agaggaaaag gcgcaggcgt ccaaaatgcc tgcgcctttt tgtatataaa attctatcct
     3241 tttttaggcg gatttagaat ccggtgtcaa cataaaagaa tcaaaaaatg tagataaatg
     3301 aatctgttac catgtttttt cctccagtcc cgaacgggaa agaaaacgaa ttgtcccctc
//
LOCUS       DAWEMX010000196         3350 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9643_length_3350_cov_12.053111, whole genome shotgun sequence.
ACCESSION   DAWEMX010000196 DAWEMX010000000
VERSION     DAWEMX010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9643_length_3350_cov_12.053111"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    996..1005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          639 a          898 c         1076 g          727 t
ORIGIN      
        1 gatgatggcg gctcctagac tgtaggtgcc tgctttcggg acgtacaaga aggcccgcga
       61 ccacttcagg atgcccacct tccgcaggga ggggaggcgc aggcgcttca tgtggtggag
      121 ggaatccctc tggggcgcct tgaaaagcgt gtttttcaga agccgggcgc agcacagcgc
      181 cagcaggacg ccgctggcat aaatcccgaa cagcacgggg gcctgcaggc gggagggaaa
      241 cagggcggag acgaacatca tgtagatcgg caggcggccc gcacagctca tcatgggcag
      301 cacggcgatg gtgaccaggc ggtcgctgcg cgtttcaatt ctcctggtgg acagaatggc
      361 gggaaccgaa cagccgaatc ccagcaggag gggggcaaag ctgctgccgt tcaaccccat
      421 gattttcatg atgcggctca tcaggcggga aaccctcatc atgtatccgc tgttttccag
      481 aacggtaatg gccccgaata ggagaaccac gttgggcaga aaggccagta cgccgcccac
      541 gcctcctacc acgccttccc ccagcaggga ttgaaggcgg ggccacggtt ccagcatgcg
      601 gcaggcccag acgctgaaca gctgggtacc cgtctggatg agcctgacca gaggatcccc
      661 gatagcgaag ctcaggaaga aaataatata aacgatgccc aggaaaataa gggaacccca
      721 tatcctgtgc atcaggacgg catccagtct ggccctccac aggttgtcct tcgcctctgc
      781 ctcccgttcc ataacgttta ccgggacgta acttaaaatc tgttcatttg acaggcaagc
      841 ctaaaagccc ggttccgcgg aaaggggcat ggaaaccagg cattccggcc gtttttggga
      901 cggggctgcc gcatgggccc cggatggggc ctcaggcttt gttttttagc ggcgcaaagc
      961 tgccaggccc tggatgaaaa ggatggccgc agcgcnnnnn nnnnnatgga gggcaggcac
     1021 tgaagcatgt acagcggatc tgtggaaccg tttccgtagg aggggaaaat aaaggccaga
     1081 atcaacgcga tcagggacag cccccatgcc agccatgatg tagcgggaat ccggcggccg
     1141 gatgctgcgg ctggccgctg cggcaggttt tcctgccgga tgccgagttc gtcagtatcg
     1201 cgtccggaga aagacgtccg ctttttcctg acgggaggca gggcgggctg cgttttcatg
     1261 gcgttttccg gtttgcggtc ggatggcgaa gaggaggctt ccccgaaggg aagccccgtt
     1321 gtaacgtgaa tcagaaggcc gcacgccggg cagcgcatgc cggttttggc gtccagtccg
     1381 gccgggtagg tgtacccgtg gccgcattgg gagcatgtgg cggattctct catgatctgg
     1441 cccgaatgga tgttagtcgt ccaagggaag caggtcctcc tggtcagcct tggtttcggg
     1501 cctctggcgg atttcctcaa gcttggcttt catctgttcg caaatgtccg gatgctggga
     1561 ggctacgttg tcaagttcct tcgggtcttt ttccaggtca aagaggcttc ctccttccgg
     1621 ggacttgctc atttttgcgg aggctccgtt gatgccgtcg cgaatggcca cgccggcggg
     1681 aatgaatttc catttgccgt ggcgaatcgc caatgccttg ccggtactgt tgatgacatg
     1741 gtagtcacgc cccttggcgg atttgcccag aagggcgggc atcacgtttt cagagtcgcg
     1801 gaaggaattg gccttgccgg gggccagcag ctgttccagg gaggccccca gatccatttg
     1861 gttgagcagg gctttgctcg tggttccggg tttgatcacg ccgggccatt tgacgataaa
     1921 cggaatgcgg gaacctcctt ccagaatgct gtatttgcct gcgcggaagg ggccggcggg
     1981 ggaatgggtg gcgttgtccc ggacggcgaa atccctgtag ccgtcatcca gcacgggacc
     2041 gttgtcgctg gagaagatca ccagggcgtc tttttccaga ccggcctgtt gcagagcctc
     2101 tgtaatgcgg cgcacacagt catccagttc cacggtcacg tcaccgcgca cgccgtgccg
     2161 gctcttgccc acaaagcgtt tttccgggca gcgcggcacg tggatgtcat gcgtggcgaa
     2221 catcaggaaa aacggttcct tggctttggc gcttttttga atgtattcaa tggccttatc
     2281 cgtaatgaca tcggcgtttt cctcatcctt ccacaaggcg cttttgccgc ccttcatgaa
     2341 cccgatgcgt ccgatcccat tgataatagc ctggttgtgt ccgtggctcc acatgagttt
     2401 gagctggtcc ttattgtcct tgccattggg aagcccgggg aagttgtgct tgtaggatac
     2461 ttcaatgggg tcgttcgggt ccaggttgcg gacattgccg ttttccagaa tcacgcaggg
     2521 aacgcggtcg cccgtagcgg cgaagataaa gctctcgtca aatccgattt cgttcgggct
     2581 gggggagata tgtttgttcc agtcaatctt cttccccttt tcccccaggc ccaggtgcca
     2641 tttgcctatc atgtaggttt tgtagccgtg ggattggagc atcttgggca gggtgggctt
     2701 cttggtatcg ataatgaggg cggcgtctcc cggcaggatg cccgtgcctt ccttgcgcca
     2761 cggatattcc cctgtgaaca gggcatagcg ggaaggggtg cagacggatg tggtggaata
     2821 ggcgtccgtg aagcggcagc cctgctcggc aagcttgtcg atggcggggg tgggaattcc
     2881 cttggctcca tagcagccta cgtctccata gccgaggtcg tcagcgtaaa tcatgacgat
     2941 ggccttgggg ggcttgaccg ttggcgtgac tgcggccggg caggccgctg ccgccgccat
     3001 ggcaagcatc aggtgtaatg cggatttatt catgttgtca ggtgtgtgtg attataattt
     3061 cccggaaagg atggtcggaa ggatgccgct gtttaagcgt gggccgaacc ttcgggtgtg
     3121 tccggaggct gttcgccggg atgttccggc ggaagcacgg gttccgtcat ggggcgcatg
     3181 ctggcatttt gccagatgta ccgcttgtaa aggaccttga tggcggctgt cagggggaca
     3241 gccagcaggg cgccgatcaa gccgcccagg atgagtgtcc agatcaacac ggaaaacatg
     3301 accgtgagcg ggtggagttc cacggaatcc cccacgattt tcggctggat
//
LOCUS       DAWEMX010000197         3297 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9824_length_3297_cov_13.378470, whole genome shotgun sequence.
ACCESSION   DAWEMX010000197 DAWEMX010000000
VERSION     DAWEMX010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9824_length_3297_cov_13.378470"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          609 a          836 c         1063 g          789 t
ORIGIN      
        1 tggtccttgt tctggtcctt gttctggtcc ttgttctggt ccttgttctg gtccttgttc
       61 tggtcctgtt gttttttctt ctcttcttcc aatttcttct ttaattcttc cagctgccgg
      121 cggtattcgt ccagggattt atcataacgg agcatttcct ccttgttgga cgctgctcct
      181 tcatgggaac cataggcaga gagagcatcg tcaaaacgtt tgacggcctc cttaacatgt
      241 tttttcgcaa cgggtatttt tgccagccgg gcatcaattt ccttaatttg gtcgtcaatg
      301 gaagagagcc gggcttgctg ctgttcccct tccggcctgg agaaagtcat ggtcttgcgg
      361 gcctgggcgg cttctaggtt gccaaggttg aagtgggaat ccgcctggag ggctggtttg
      421 ggagaaagga gcgcctggga gaaagcttcc gcggcttctt tggggttatc cgctttttgt
      481 tccaggcatc ctttggcgaa ttccaaggcc tggcgcgttt cttccgccag gtcgggaaca
      541 gcgagcattt catcaatgga tttgacggct tcttccgttt ttcccgtccg gatcaaatcc
      601 gtgacattgt ccagggcgtg cgtgtccgcc tgcgcttcct ggcccagcaa tccggcgcag
      661 aataacgccg cagcagtcag agatgtcata ccgcggcgtc cggaacggcg gaaggaacgc
      721 cagcgtgttc ccgccagaag ggtgaggagc aggcagatga gggccggaac ggcaaagatg
      781 atgtagcggt cattcggcac gcggcgcact tcttccttgc cttccgtggt atccagccgg
      841 tccgcgatgt ccttgacgaa gcggttcagc gccgctccgt cggagagttg gacatattgg
      901 ccttccgttt gcctggccag gtattgcagg ctttccggat tgagcttgga gacgacgtgc
      961 tggccacgac gatcccggta caggccggag ggggattgct catccggaat agtggtgccg
     1021 atagtagtac cgatacccgc cgtcacgatg ataagctggt gctgccgtgc ggcttccgca
     1081 gtcttatagg tgatgttgac ggtgtcttcc ccgtcgctga ggatgaccag ggcgtttttg
     1141 gcgtccgctt ccttgtcccg tttgaaggtt tgaagggcca gccgcacaac gttttccagg
     1201 ttggttcccc cttgggagac ccagccgaat tgaagttgtt caatggtttc ttttaatgcg
     1261 ttatggtcat gggtaagggg catgagcagg acggcgtccc cggagaagat gatgatgccg
     1321 aagttgtccc ccggcagggc gtccagcagg tcgtaggcgg cggtttttgc ccttcccagg
     1381 cgggtcgggg aagcatcctt gctgagcatg gaacgggagc agtcaatagc aatgaggatg
     1441 ttgcgcgatt tggggatttc ctttacctcc gtgtagccgt ccaccgggcg cgcaatggag
     1501 aggatggcga agatggacgc cagcacggca aagatgacgg ggagcacccg gtgccaggtg
     1561 gccggagcgt gaaccagttc ccgccggtat tccggcgaaa cgagtacttc ccattttctg
     1621 gaacggcggc gccacagcca ccaggccgcc gccgcgagca gggccgggag aatgagcgcg
     1681 tacagaacgt tgggataaag gaaagtcatg acgggtgaag atcggtatta cggggcgggg
     1741 gccggtttga gcacttgcag ggagaggccc agaagcgcca gcgcggctcc cgttacaagg
     1801 aagtacatga agtattcttc aatgtggcgg acagtttgca ctttggcctc gcttttttcc
     1861 agcttgccga tggaggtgaa cgctttattg aaggaggcca tattggtggc cctgaagtgt
     1921 tctccgcccg tgagctgggc tattttccgc agggttggtt cgtcaaattc actttgcacc
     1981 accataccgt tcgccagggt tccttcttcc gtgcctacgg caatggtata aattttgata
     2041 cccagcttgg ccgcgttttc cgccgccacc agcggggata tctggccgga gttggaggca
     2101 ccatccgtca ccaggatgat gatttttgat ttggtgtcct tacggtcgtc cagccgggtg
     2161 gccgccgccg caatggcgga gccgatggcc gtgccgtccg cctggatcat gcgcggatgg
     2221 aaatcccgga tgatgctgtt gaccagggcg tggtccagcg tgagcggaca gaaggatttt
     2281 gttttcccgg caaatcccac gataccgatg cggtcgtccg gacggctgtc aacaaattgg
     2341 gtgatgacgt gtttggctgc taccagccgg ttaatgggca ttcggttgag aaccatgtcc
     2401 ggcgtttcca tggagtagga aaggtcaaag gcgatcatga tgtcaatgcc gttgactgtc
     2461 cggaaggttt tatcttccac gtgctggggg cgcgccaggg cgatggcgat ggcggcagcc
     2521 gccagaacga agcaaagggc ccctattttc cctgccaggg attgcggagt gcgcgccagg
     2581 gaagcgatga agcgcacagt ggggtaggtg atggagcctt ccgccccgcg tctgcggcgg
     2641 aggatgatga ggcacaagat gaagggtaag gcataaagcc attcaggttg tgcaaattgg
     2701 aagtgttccg tcatggtatg ctagcgttgg ttgaagcggg atgaagattt tttgacgaca
     2761 aggtccgttt cttccttcgt ccggtgggca tccgcttcta tatcattgat gagttggacc
     2821 gtggcgtccg ccaattcgtt gaccatggaa tcgttttccg gggtgtccgg ttcatatttg
     2881 agagaagcca tgcggtccag cagatcacgc gtgggggcct ggagttcgga aggcagcgcg
     2941 gtgagcgcgt ccgcccggcg gttgaattcc tgctgggttt catatagtgc cgggtctttg
     3001 gtttctccta ccaggtattt gcggaggatc agggaaaatt ccacggcggc ctgcctgagg
     3061 gagggatgcg tgttccggag catcgagatg tcccggatag cggtttccga cgggctgagc
     3121 ggcgcggcgg ggatgcgtcc cttcctgcgg atgagcacga tgacggcgat agcgacggcc
     3181 agaaggatgg aggccaaaat aatccagatg ccccacgcat cccaaaagcc cgcttggaag
     3241 taggcatccg gggattgtgt ttccatcagg gagggaaacg ccggcggttc cggaatt
//
LOCUS       DAWEMX010000198         3280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9879_length_3280_cov_11.112558, whole genome shotgun sequence.
ACCESSION   DAWEMX010000198 DAWEMX010000000
VERSION     DAWEMX010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9879_length_3280_cov_11.112558"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          700 a          996 c          844 g          740 t
ORIGIN      
        1 gcggcaagcc cctcaatccc ctggtcaacg caggggccat ggcgacggtc agcctggtca
       61 acggttcgga ttccgatgaa atatggggaa acatgatcca taatttcaat aatttcgcca
      121 acacggctct gaccgtgaac caggagattt acaagtcgga aagcgctacc aaccagcaca
      181 accgcggcat tgcctggctg ctggacagct acggctattt ttacaatacg ccgcccatga
      241 ttgtggattt gtacacacgc atgtgttccc tgaatatcac cgcttcccag ctggccctga
      301 tgggtgcctg ctacgccaac ggcggggtca atcccgtcag caagaagcgt gtggtgaaag
      361 aggagaacgt tccccccatt ttggcggaaa tgtgcatgtc aggcctttat gattccacag
      421 gggactggat gtacaaggcc ggccttccgg ccaagtccgg cgtgggaggc ggcctggtgg
      481 ccgtagctcc cggcaagctg gccatggcgg ctttttcgcc tcccctggat cccgcaggga
      541 ataccgtcaa ggggcaggcg gcgcttcaat ccatcatcag ggaactgaat ctaaaccttt
      601 tccggagctg atccgggatg agaaggtaaa cccgcggagc caaaatccga aaggattccc
      661 gcgggtttac tgtatcctgt ccagcccccc gaggcagacc gcagtttctc gactgcggat
      721 ttcagccata aaaaacagcc ttccggcaca gtatgctgaa aaacggatat atccggcgga
      781 caacggccgc atcgcctgcg ccccggcagc cgcaggcaat ctttccccgg cgccccgcat
      841 cagctcctgt ttgaaaagtt gcacagctgc atggctacgc cgtcttccat aacccggaag
      901 ccgtgcttct cataaaaagc ggcgtttctg ctctcctccg gcatgatttc aagatacagg
      961 tagtccctgt atttttcctt aaccatttcc accatttttc cagcaatccc cctgccctga
     1021 taggcgggat gcaccagaac ataatgcatg taggcaacca tctcgctgtc gtccagcacc
     1081 cgcaccagcc ctgccaggcg gtcaccatcc caggccgtaa caacagtgga cgaattcatg
     1141 agagccttga acagacgccc cggatattgt ccggaaatcc agccaacgga caggaacaat
     1201 tcttgaacat cttccctgga aaatttcttc tcttccgtaa aaacaatcat tttttcttta
     1261 attaattatt gattatccgc ctgtaccgtc gcggccacac aaccgactga gcgggaaacg
     1321 cctttccctc ggccggattg acaccctgtt tcaaggtttt atctgttccc actgccgcag
     1381 tcaacaccgt cttgtccgag ggttttaccc attccaagga catggcggag cctgtgataa
     1441 gaacgcgcgc gccggaaaca ggcaaccctt tccccagtca gcagctcgtc cgcccctgcc
     1501 tcctcagctt ccggggaagg aagagccgca ccaggaacac acatcttctt ttcagggaaa
     1561 cgatactcga atacgggaga cggcattttt cctggaaccg attccccaaa aacggaaaac
     1621 tccgggccca ggttcataac attgtgaaaa acctttctcc gtcatttgtt taaggacttg
     1681 tcaccacgct tcttttccct agaatcctcc cggtatgctt gacgccctcc aaaatgcctt
     1741 tttccagacc ggggacacgc tcacatcctg gctgtccgct ttcagcagct ttctgtgggg
     1801 gtggcccctg ctcatcatgc tgctgggcac gcacctgtac ctcaccgcca tcctgcggtt
     1861 tccgcagcgt tatctgctta aggccctgaa attgtatttt gtgaaagatc atacggacaa
     1921 gggagatatc tccccattca gttccctgat ggtggccctg gcagccaaca tcggcgccgg
     1981 gaatatcatc ggcgtgggcg tggcgattgc agccggagac cccggcgccg tcttttggtg
     2041 ctggctgacg ggagttctgg gaatggcaac ccgttattcg gaaggattgc tcgccatcaa
     2101 ataccgggtg gaaaacaagg acggcaatat gagcggaggc cccatgttcg tgctggaacg
     2161 cggtctgaac agcaaatggc tggggacagc cttcgccgtc ttcacggcca ttgccgcctt
     2221 cggaatcggc aatttgacgc aagggaacgc agcggcggaa cagctccacc atgccttttc
     2281 cattcccttc tggggaacag ccgccgttct gacggcattg acggctctgg tgatgctggg
     2341 aggcatccag ggcattgcca gggtatgcgc tttcttcgtg ccgttcatgg ccgtcatcta
     2401 catcatggga tgcctgtata tcctgaccat acaggcagag tatattattc ccgccatccg
     2461 gtatattctg gactgcgcct tcacgggaga agccgccgcc gggggcgcag caggagcggc
     2521 cgtcatggcg gcaatgagaa ccggtgtggc ccggggcctt ttttccaatg aggccggcct
     2581 gggatccgca gccatcgcct ccgccgcagc gcagaaccgc aaccccgtca ggcaggcgct
     2641 catctcttcc agcggcccct tctgggatac cgtcattatc tgtgccctga cgggaattgt
     2701 gctgaccacc agcatcatcg cgcatccgga catttcctgc acggacggcc ccaggcttac
     2761 tacgctggca ttcagcaaaa ttccttacat aggctcccct ctgctcacgc tcagcctggt
     2821 tacgtttgta gtttccacca ttctgggctg gtcctacttt ggagaaaaag cgctggaata
     2881 cctgggaggc atccggctga tcatgcctta ccgcgtcatc tgggtggcgg cggtcttcat
     2941 cggctgcgtc tctaaaatag aactggtatg gatttttgca gactgcgcca acggactgat
     3001 ggccctgccc aacctcattt ccctgctggc actttccggc gtgcttgtcc agcaaacgcg
     3061 gtactacctc tggcagcaca ggctggacga ttacgacgaa tcccacatac cggaaggaaa
     3121 ataacgattt ttttaactcg gattcaaata attcttgcct ttcggcttcc ggtagtattt
     3181 acttcgccgc gcagtccctg caaacctaaa agtcgcgttc gtctagcggt ccaggactcc
     3241 cgcctttcac gcgggcaaca cgggttcgag tcccgtacgc
//
LOCUS       DAWEMX010000199         3262 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_9934_length_3262_cov_11.748675, whole genome shotgun sequence.
ACCESSION   DAWEMX010000199 DAWEMX010000000
VERSION     DAWEMX010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9934_length_3262_cov_11.748675"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1228..1237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a         1040 c          921 g          567 t
ORIGIN      
        1 accatgtgga ccacggcaaa acatccctgc tggactacat ccgcaaaacg cgcgtggcca
       61 agggggaagc cggaggcatt acccagcaca tcggcgccta tacggttgac tacaacggca
      121 gcacgctcac cttcctggat acgccgggcc atgccatctt cacggaaatg cgcgcccgcg
      181 gcgcggacgt cacggacatc gtggttctgg tggtggccgc caatgacggc atcatgcccc
      241 agacgcggga agccatcgcc cactccaagg cggccggcaa aaccatcatc gtcgccatca
      301 acaaatgcga ccttccggcc gcagaccccg tcaagaccaa gagcggcctg atggaagaag
      361 ggctggttcc cacggacttc ggaggcgatg tggaatgcgt ggaagtctcc gccctgaccg
      421 gggccggcat tgacgacctt ctcggccttc tcgtcctcca gtcagaagtg ctggaactcc
      481 aggccaaccc caaggccaac tgccgcgcct ccatcatcga ggcccgcgtg gaacccggca
      541 cgggcagctc cgccacggcc atcgtggaaa gcggcaccat ccgcgtcggg atgcccttca
      601 tctgcggccc ttacgccggc aaggtgcgcg ccctggtcaa cgaccacggc gaacgcgtca
      661 aaaaggtagg gcccggcatg cccgtggaaa tcaccggctt ctctgaaacc ccgaacgtgg
      721 gggacgaact ggtggaaatg gaaaacgaac gcgccgccaa aaagcttggg gaagaacgcc
      781 aggaggaact gcgcaagcag cgcctggccc agccccgcaa ggcacgcatg gaagaactgc
      841 tcgccatgat gggggacggc acccagaaag cccagctcaa aatactcctg aagggggatg
      901 tgcagggttc cgtggaagcc atcaggaaag ctgttctgga catccagtcg gacaaagtgg
      961 aatgcgtctt cctgaacgcc tccgccggcc ccatctcgga atcggacgtt ctgctggcct
     1021 cctcctcgga cgccgtcatc ctgggcttca acgtcaaggt ggaagccaac gccgtcaaac
     1081 tgctcaagcg ggaaggcgtg caggtaaaac tgtattccat cgtttacgaa ctcattgacc
     1141 aggtgcggga tgccatgctg ggccttctgg aaccggaaac gcgcgaaacc atcatcggcc
     1201 acgccaaagt gctccaggtc ttcaagcnnn nnnnnnncaa caagggccgt gcggcaggct
     1261 gcatggtgga ggacgggaaa atcctccgca gctgcgaggc gcgcgtcatc cgcgacaaga
     1321 cgcccgtctt tgacggtaaa atgtccaccc tccgccgctt ccaggatgaa gtggaagaag
     1381 tcaaggctgg tctggaatgc ggcatccgcc tcggagactt caacgaatac gaaacgggcg
     1441 acatcatcga atgctacacg ctggaaaaaa tccagcaaac gctgtaaccc ccaacaaaaa
     1501 tcatccaaca gggacggagc tccttgcacg ctcctccggg cgtccaggaa acctccgtcc
     1561 ctgtcctttc ccttattcct ccttctccga ttgaaccaat cggtcccttc atcactctat
     1621 cccttcatat gagcagacgc actgacaagg taaacgaact cctgcgccgg gaaataggca
     1681 ccaccattca gcgggatttt gaatttcccg gaacaatcgt caccgtgatt gaagtggaag
     1741 tgacggacga tctgaaagag gggaaagtat gggtgggcgt cgtagggaaa atggctccct
     1801 cccaggtgct ggaaaaactg aactcccgtc acggcctcat ccagtccgcc gtggccaggc
     1861 gcgtggtgct gcgcaatacg ccgcgcctga ccttccgtct ggacgactcc gcccagcgcg
     1921 gcgttgacct ggtcaacctt ctggaagaca ttgacaagaa cctgcccaaa gccccccctg
     1981 ccgatgcgga aaatgacggg gagtaatatt cttcccctgg ctaaggcacg gtgtccccgg
     2041 ccgtaccgcg ctggatgcgc cgggacagcg gagggccgga acacgcagaa tcacgcttcc
     2101 ggaaaaaatc cgtaacgggc ggcaaggcag cagggccgga aggtccctgt tcccgtaagg
     2161 aatcatcatc gggaaacgga aagagaaacc agtgattccc tatctcgtca cctttaatct
     2221 cagaaaccgg cgctccggag agccttttcc cagcgcgacc gtatcgcgga attccccgca
     2281 ggccccgtcc ggagtgacgg ccccttcgtc agcccattcc tccaggtcgg aagaactttc
     2341 cacatggaac gtcacatccg tcgcctccgg attcacaggc caggaaagca cgaggtattt
     2401 cccgcccgct tcctcgcgga cagccagctt cgtcacgctc ccgcagggct ggttgggatc
     2461 catgcccgta gcatatttca tcagattgga aattccgtct ccggcggggc aggcatccgg
     2521 ggccatttgc tcatccggcg tcccatccgc aaaatggtcc tgcttccact tgtcgtaccc
     2581 cgccggggca ggagcagctt cctggcgcag cgtgaatgtc ctctgctccc cgccgcatac
     2641 gacggtgaca acggcttccc gctccgtttg cagcccgttt gcttccacgg caatatccag
     2701 cactgaggtt ccggaaccgg aaacggggga aaccgtaacc catggctggt cgcaggaaac
     2761 tgtccaggga agatgcgtct gcacggtcag ccccgtaccg gaggcggcgc atcctaccgt
     2821 ttcctccgtc ttttccagat tgaagggatt cacgtattca taggaaaacg cgctggcggg
     2881 aacgggttcc ctgccgctgg tttccggccc ctcccattgc agggaaaggg aggaggaagc
     2941 cgccttcccc acatagccga tgcggatggg atgcagcccc gcggtaagct tcaccgtctg
     3001 aacggcattg ggctgcacat cgccggaaac gccttcccgc gcgttggagg cagcctgcgt
     3061 tccgggagta taggcataat ccgcatcaat gagctgcata tcgtgcaaat ggacaaaagc
     3121 cctggagcct tcattggaat ccgtctgaag gtaaaattga tattccccgg tgacaggcac
     3181 cctgagatag cccgtcaatt ccaccccctt ctgaccggaa agccccgcct tgacgctgag
     3241 cacgtcggaa acatccgtcg ca
//
LOCUS       DAWEMX010000200         3221 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10066_length_3221_cov_11.076121, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000200 DAWEMX010000000
VERSION     DAWEMX010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_3221_cov_11.076121"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    839..848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          772 a          942 c          809 g          688 t
ORIGIN      
        1 ttcgggaaat aagaaacctt tccatgaaaa ccgcccgccg cgtccgggac tatagcgact
       61 ggtatgaaat caacacgcgg tccggaaccg gcaagacatt cgcttcctac gcggcagccg
      121 tgaaaatcct ccgggaacag gaacagccgg ccaatacccc tgtttcccgc tacctggacg
      181 acattgaaaa actgtacacc ctcccggcca gggctcccgt gccctccctg catgggaagg
      241 attaaggaat taaggaaagg cgttctgcgg cggagccatc ctgccgtccc catcaagagc
      301 gccgcacttc taaaaaacgg cttcccgtgc cgtgcaatgt tctatcttca cggcgtcaca
      361 aaatgccgat gagttctccc cggaagccgg atggtgcgga aatctggtgc tatgaagaga
      421 attgcttgcc ggaacatgcc aaatcaccgg cagaaaccat gcatcacgtc atgaaccaat
      481 agacggtccg tcagcgtttt gaatcattct ctgcatcagc gctcatcccc gccgcagaaa
      541 cgcgcatttt tttatgtggt ctttttttat tcagaaagat tcagggattg tctttcccaa
      601 gatgatttgc aatcatcacc ccgaattatt ttcatatcac tacataatat ggacacgtca
      661 ttgaaactcc acggcctggt ggccgccgta cacactccgt tcaaggcgga cggttccctg
      721 aacccttcca gcgttgacgc ccaggccaag ttgcttgcct cccagggcat caagcttgct
      781 ttcattaccg gcagcaccgg ggaatcctcc tccatgcagc ttgaagaacg caaggaaann
      841 nnnnnnnnaa gcctccgcca agcatggcgt ggaagttatc gcccataccg gttccaacag
      901 cgtctgggac gcccgggaac tggcctcttt tgcccaggaa tgcggattcg tggccaccag
      961 ttccctggcc ccgtcctact acaagcccgg cactgttcag cgcctggtgg aatgctgcgc
     1021 cttcgccgcc tccggcgctc ccgacctgcc ctattattac tacgatatcc ccgtgctgac
     1081 gggcgtacgc ttcaatccgg tggatttcat caggctggcc aaggaacaga ttccaaattt
     1141 cgcaggcatc aaattcacca atccggatct ggccctgtac cagaccacgc tgaattacga
     1201 cgagaccgtg gatattccct ggggtgtgga cgaatggttt acgggcgccc tttccgtggg
     1261 ggccaagggc gctgtgggga gctccttcaa ctttgctccg gccctgtacc agaaactcat
     1321 gaaagccttt gcggaaggcg atgtggaaac ggcgcgcgac tgccagtgga aatccgttca
     1381 gatgatcaat atcctggcct ccaagggcta tatgggctgc gccaaggctc tgatgggctg
     1441 gctgggcgtc gatcttggcc ccgcccgact tccgcagggc aacccgaccg cagatcagct
     1501 gaaggaactc cgttccgaac tggaaggcat cggcttcttc cagtgggctt taaactgaac
     1561 cccggaacct gactgacatg gctgcaactc ttgatatgcc ctctttgggc aagttctacc
     1621 gccgccagct tctggaagac gttctgccgt tctggttccc ccgcgcctac gacgaaaaaa
     1681 acggtggtct ttaccactgc tttgacgcag acggcacgct ggtggactcc gacaaatccg
     1741 tctgggccca gggccgcatg gcatggatgc tgctgacaat gtacaacagc atcgaaaaga
     1801 atacggactg gctcaaatgg gcggaaagcg ccttggaatt cctgaaaacc aagtgcgttg
     1861 acccggcgga cggccgcatg ttctttcatg tggctgccga cggcaccccc atccgcaaac
     1921 gccgctacgc ctacagcgaa tctttcgccg ccatcgcctt tgcagcgcac gccagggcga
     1981 ccggcagccg ggattccgcc cgtgaagccc gccactggtt cgacatcttc acggacaact
     2041 gcttcactcc cggcaaaatg gttcccaaat tcaccgggga acgccctacg accggtctgg
     2101 gcacccgcat gattaccctg aacacagctc aggaaatgcg caagtacctg gaagatgacg
     2161 acggcttcta taccggctgg acagaccgct gcatcaacga cctccgcacc ctgttcatga
     2221 aacctgacat ccaggcagtc atggaagtgg tgggaacgga cggttccatc atcgaccact
     2281 tcgacgaacg caccctgaac cccggccaca ctacggaggg tgggtggttc gtgctggaag
     2341 aagcccgcca tcgcggcaat gaccccgaac tcatcaaggt gggctgcgac atgatcgact
     2401 gggcattcgc ccgcggctgg gacaaggaaa acggcggcat gctgtactac acggacgtgt
     2461 acaacaagcc cgttcaggaa tactggcaca acatgaagtt ctggtggccg catgatgaag
     2521 cgctcatcgc catgacgctc gcctacaagc tcacagggga agaacgctat gccatccgtc
     2581 acgacatggt gcgcaactgg gctttctccc acttccagga cgttcagcat ggcgactggt
     2641 tcggctatct gaacaaggac ggttccaggg ctaacaccct caaggggagc ctctggaaat
     2701 ccttcttcca ccatccccgc gccatgtggt gctgcgccca ctactgcggc gccatttaag
     2761 ggaaaataaa atacatcgtt acttctgaaa gccgtttgct aaatgcagca aacggctttt
     2821 acttttgaag acagtaggaa agatatttca aaaatagttc cctatttaga ttgcctccta
     2881 ttctacagta ttttatcata ttccatgaga aaaatctgcg tattttttca tggagctaat
     2941 gaatccaact cttcacatcc atgaaaagca tcattaaatt caggtgcgat agacactaaa
     3001 gcaacaaaag ggaaatatca tgactggtga aaaattcaca ttgaggcaat attccgttag
     3061 cgccatcttg gggctgatag aagcagatga gttcgttata ccggaaatcc agcgcccctt
     3121 tgtctggaaa cgaagccagg tacgggattt aattgattcc ctatataacg gctaccctac
     3181 gggctatatt attacttgga aaaatcctga cgtgaaaacc a
//
LOCUS       DAWEMX010000201         3152 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10303_length_3152_cov_7.991928, whole genome shotgun sequence.
ACCESSION   DAWEMX010000201 DAWEMX010000000
VERSION     DAWEMX010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10303_length_3152_cov_7.991928"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1582..1644
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          693 a          637 c          925 g          834 t
ORIGIN      
        1 ccgttctccc cccccctaca ttctcttagt tagtttcctt cttgtaagat gtcgtatttt
       61 ttgtatgtat tagtgttact atgttttctc atgcttttgc cgccggggcg cttcttggaa
      121 ctgccatttc tccgcttgtt tgcgtatttg ccgcagaaag atcgcctgtg gcggcggctg
      181 aagatgtagt ggcgcgtgta actccctcgg caaagggttc tgtcttgttc cacttaaatc
      241 cggatcaggg caacaagatc actatttccg gtattgccgg aggtatccgg gtggaggcgt
      301 cagatgtgcg ccgcttggtg gcgggttatg gatggtattt aaagaatata gccaaggtcc
      361 atttttcgtg gaacggaaat cggataacgc ttccttcccc tttgccggtt cccgccagtc
      421 cggttacggt agaaagcccg tggaatatcg tttttgccta taattactgc accctttctt
      481 atacggcggc tttttgggat tggaacagat ggcagcggga gattgatttt ctggcgttga
      541 atgggtttac gcatgcgctg gtaacggcgg gtttggaaaa gacatgggaa gattttctga
      601 caggcttggg gtatccccgg gaaaaagctc ttcgttttat ccctaatccg gcatttgccg
      661 cgtggtggaa tatgggaaac ctggaaggcc acggtggtcc gctgagccag caacagataa
      721 ataaaatggc ccagatggga aggcgtattg tctcccgtat ggaacagttg gggatgacac
      781 ctgtacttca ggggtatgtg ggatttgttc cttctgactt tcaggaaaat gtccggatag
      841 acggattgaa gcttatccct cagggggaat gggttaattt caggaggccg tgggtggtgg
      901 atcccacttg cgaggctttt cccaaactgg ccgcggactg gtacaaggct ctccgcaagg
      961 tatacggcat tcccggaaag atgtttggcg gggatttgtt tcatgagggg gggcggaagg
     1021 gggatattga cgtaacgcag gcggcacagg cagtgcagaa agccatgcaa aaggcttctc
     1081 cgggggcttt ctgggtgatt caggcctggg gtggaaatcc tacccgagag ttgttgtccg
     1141 ggttggatcc ggagcgtgcg cttgtattgc agcttaccaa ggatatggct aacgggggaa
     1201 aaaatttaag gacttttaat ggtattccgt gggtctggtg cgaactggcg aactttggag
     1261 gcaatacggg aatgtacggg ggggttcccc ttctgtcccg gctcggaagt gagttgtccg
     1321 gctataagga taaggggctg gtgggcatgg gaactttgtc cgaagggctt gagacgaacc
     1381 ccctgcatta cgccctgttc agcgaccgtt tgtggactag ggaggatatt tctgtccggg
     1441 agtggcttgg aaagtacgcg caccagcgtt acggttccgc accaaaggct gtcgtaaagg
     1501 cgttggaagt tctggcttct tccatttata accccgttcg ttctcaggaa gggtgtacgg
     1561 agtctattat ttgcgcccgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnacatgg tccagcgggg agcgatatta tcacctgggc
     1681 gatattgtaa aggcggcgcg cggttatctg aaggccgcaa atgatcagcc gaatttggtg
     1741 aaaaaggaga cgtttcgtta tgatttggtg gatgttgtgc gtcaggctct tgcggacgct
     1801 gctttttatc agcttcaaca ggtcaggagt gcttttgatt ccggagattt ggctgtgtac
     1861 aggaagcagg taaagcgttt cctgtctctg atttcagata tggatgccct tttggcgacg
     1921 gatagccagt tcctattagg aacttggcag aaaagggctt tggattgggg tgattcccgg
     1981 caggaaaaag ccttgatgga caagtctgcg aagatgctaa tcactacgtg gattgatcag
     2041 gttccccggt ctctgaatga ttattccaat cgtcagtggg ccgggcttgt ttccgatttt
     2101 tatttgcctc ggtggaagaa tttttttgaa tttcagatgg atgtcctgac cggaaagaag
     2161 acgcgtgatg ccgcccatgc cgcatttatg gataagatgg ttcgggatga actggccttt
     2221 gccggaaatg ggaaaatata ttctgccaaa ccggcggggg atactttgac tgttgctaat
     2281 cgtgtgatga atacccaccg ggaaatgttg gacgccctgg gtgcggaaga aaagcattcc
     2341 tcgggcagtc catgggaact ccagcaaggt tctccgttgc agtttgatgt gacggatcag
     2401 gtgactgctt ctggcacata tactgctact ttccggtgga agaatggccc cagcgccttg
     2461 aaaatccatt ctgtcagatt atacgagggt aacagggagg tggcttccga tgttcatgag
     2521 ggcagaactg gcgtggaaaa taaggataat atttacagac tggaattgaa gaagtacaga
     2581 accaaccttg attcctatat cctgaaggca gaagtaagcg gtgtttctca gggcgcttcc
     2641 aaaggagaaa tggtgttgaa aaaactggtg aattgagaag tggacaggaa gttgtgcagg
     2701 gatgggagaa aaggcggtta tacgtgcagg ctcttccatt tgttccggaa tcagtttgtt
     2761 tccctcgccg aaaaaaggaa gaagggggat ttcccccttt cttccttgaa agatggcatg
     2821 atgataaaat ttatcggcga tggcgtaaca atagagtgct aagaccgaac agacccagcg
     2881 tagcggttgc cggttccgga accacttcat tacttgtgga gaagtttact gccgacaaat
     2941 acatgccctc ccaagagttc agggtattgt ttttataggt gccggttctg cagccgctat
     3001 gcatgtccgg agaaaggctg tttaaataaa cggtttcggg aagtgttgct tccttcctat
     3061 tcgtggcggc ggacataacg gaagaagacg attgggtcgc agaaccaaag tggttgttac
     3121 ctgcgttgtt ggcggtgtta gtgttcgtgg tg
//
LOCUS       DAWEMX010000202         3080 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10534_length_3080_cov_9.306446, whole genome shotgun sequence.
ACCESSION   DAWEMX010000202 DAWEMX010000000
VERSION     DAWEMX010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10534_length_3080_cov_9.306446"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          691 a          816 c          815 g          758 t
ORIGIN      
        1 aaggggggct gaaggcttga tacccggaga ttccacacgc aaggctatta tcaaagagga
       61 accattggct catcagatct cctgcaggaa aaacctctgc tttgaaatag ttcagctgct
      121 ccgcaagcgt agaggaaatc ggaatcatac gcgccgcgtt cgtcttggta atgccgcggc
      181 tgagctaaat ctctcgcgcc cgcagggata ttccctgcgg gcgatagccc cggaagtttg
      241 ttcctaggaa taggatttct ccaggatgct gaccacgtcc gccggagtca acttgcaacg
      301 gtccagctca aacagattgc ccatggtgga aaaagccgtt ttggcgtatt cgtcaaataa
      361 atccggtgta atgccgaatt cggacatctt cagcttgtct acgccgcagg cttccttcag
      421 gctgttcaag ccgttcagga aaccgtccac ggaggcttcc tgtcccatgg ctgcggcaac
      481 tcttgcgtaa cgttccgggc aggagggagc ataggcttca tggtaggccc aggaaatcat
      541 aattagcccc gcgccatggg gcagggaagg atggaaggcg ctgagggcat gttcaatgga
      601 atgttccgac gtacagcagg aaatggtttc cacaaacccg gaataggtat tggccagcgc
      661 cacataggcg cgcgcttcca gatcatcccc gttatttacg gcgcgcggaa gatatttggc
      721 aatgtattca atagcctgga gcgcgaacat gtcccccatg ggggaggcga ttgtagccat
      781 atatccctcc acggcatgga acaaagcgtc aaacccctgg tatgccgtta atttgggagg
      841 tacggaaagc atcaactccg gatctacgat agacatggtg gggaaagtac ccttgaaccc
      901 gaaaccgatc ttctcctgcg tgtcctcttt cgtgatgacg gtccacggat ccgcctccgt
      961 tccggttccc gccgtagtgg tgatgcagac gatgggaaga ggtttgcagg gaatgggaag
     1021 ccccttgccg gaacctccct ggatgtaatc ccagtaggtt cctggattgg ccgccatcac
     1081 ggcgatgctc ttggcggaat ccatgctgct gcccccgccc aggccgatga cgaaatcaca
     1141 gcccgtttcc ctggccaggg aggaggcttc cattacgtgc tccaccacgg ggttgggctg
     1201 cactttatcg aaaacaacgc tttccactcc ctgttttttc agaagagcct gtacgcggtc
     1261 cagatacccg aggcgtttga cggacgaccc gccgatgacg atcagggcct tggtgccggg
     1321 caggggagcg gaaccaagat tgtccaaaga acccgcgccg aaaaagattt gcgcgggcat
     1381 gaaaaattga aatggctgat acatgccctt aatgtataaa acggcggctc cggacgtcaa
     1441 ggatgctctg cgcattccat gaacagggaa accgccgttc gggcaacatc ttttgtctga
     1501 agaaagtcag atgtcttcaa aaaaatcgtc actccgggct ggggccggct tgggaggctt
     1561 cggcacatca gttccgatgg ctccgggatc atccatttca cggctggtcg taatcgtggt
     1621 ggagggagcc ttctttttgg aggaacgcat ggcaggacgc ggtgcagagg atgaagagga
     1681 accgtcagcc atgctttctt cttccggaga atccgtcatg ctcttcatgc tctgaaccag
     1741 tttatcccgg agggtgcgga taccttgttc ccggcgcacg ggatccaccg tcgtccggat
     1801 cagcgtcatc atattcctcg tggcgtttgc ctggtctccc aagtcgaagc aatccagttc
     1861 gcagcgccag cgcaaacgat ccagaacggc ggctccctgc tcatcaaatt cgtcttcgga
     1921 cgcattggcg atgcggcctt gcatgccgga tttcttgccg gaggaaatgg catggctgat
     1981 ttttcccaga gccacttcca tggaaattct cttgttccac gcctgcctga gggcgggaag
     2041 attgcgggtc ttccggtatc cggtgaaaaa cagcttgtca aaaatttcat cccggttttt
     2101 catggaatcc gggaaagcct tggaagacat gctgctgatg cccagggcct cccccacttc
     2161 cgtattaagg acggattgtt ccgcaatacg ggcgccggct cttccggctg ctccattgtt
     2221 gtttttggaa gaggaaggtt cgacaaaatc agggtcggag caggcggaca ggtaggcatt
     2281 cagcaaatcc atggccggct tctggaactc cgtaacgtcc agggtttcct ccgctgttcc
     2341 tccggtggaa tgggaggtgg cgttcattaa attttcctta agacgagcct tgaggacgag
     2401 aactccccat tgatattgaa gcattaatcc tttgcgggca tttttatcct ggtacttctt
     2461 cgcttcacgg tccatccagt ccttcatgga attggaggcg attttggtgc tcttcccctg
     2521 gctcttgtcc ttctcctttt ccgtttccat cagaaacttc ttggactgaa tcagcaggtc
     2581 atatgcggcg ccgggatccc tgccggcgga attcagtttg ctgatagcgg cattgattac
     2641 cttggtttct gcagacctgt tcagagcgag caaattatcc aaatccttga ttaccttctt
     2701 cttggcatcg ttatccatgt actgcgcata acaaagggaa cccaggctga ggctcccgac
     2761 caggcaaaga gaaacgaaag tattcatgct ttttttatta tgaaggatga aaggtgatct
     2821 ggcaagagag aaggaatctc aaaaagataa aatatccttg caaactaaaa atatcatgat
     2881 tccgcgctct ggcagtaatt atttcttttg tttcttctct ttaaggaact ttcttcccaa
     2941 cgggctttga tacaaaaatt ccgtttcttc ggtttttccc tcctcccgat caaccaactc
     3001 catcatttct ccttcctcat atcccgccag cttcaggtat ttaaacactt cggtggcttt
     3061 gccggggtag ttgtccaaca
//
LOCUS       DAWEMX010000203         3040 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10686_length_3040_cov_8.045896, whole genome shotgun sequence.
ACCESSION   DAWEMX010000203 DAWEMX010000000
VERSION     DAWEMX010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10686_length_3040_cov_8.045896"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          652 a          831 c          890 g          667 t
ORIGIN      
        1 tgtccgggtg aggcgcatgg cttcctgaat atccggttcc agcttgtcct gggtcagccg
       61 gctccagcgg atgggacgct ccctgtccag gaaagggtgg gcggaagtgg ccgccgggga
      121 gggaagggcc acggagagga agaggaaaaa gagatgaaga gactgcctga gtaaactcat
      181 ggcggccatc ctaccggaag ccggtttctc cttcaagcct tgcgcttttc gtgtctgcat
      241 caggggaaaa gccggggccc ggtcttgaac ggcttcccgt ttgcatgtat gaatcaaggg
      301 gatgtccacc aaaatctttc tctatgatac tacgttgcgg gacggcgcgc agagtgaaga
      361 cgtcaatttg agcgcgacgg acaaggtccg catcgcccgc cagctggatt atctgggcat
      421 ggattacatt gagggcggct ggcccggcgc caatccggtg gaaacggaat ttttcaacgc
      481 catgagaggt gtcgggctaa ggaatgcaaa gctggccgcc tttggaagca cgcaccatcc
      541 ttcccatact ccggagacgg accccacgct gaccgccctg atatccagcg gtgcgcgggt
      601 ggccgcggtt tttgggaaat cctgcccccg ccatgtggaa gtggccctgg gcatttcccg
      661 ggaacgcaat ctggaaatca tcggcaattc catttccttc cttaagaaaa atatggaaga
      721 agcctttttt gacgcagaac acttttttga cggcttcaag cgggatcagg aatacgccct
      781 ggctgtgctc cggacagcct gggaacatgg agcggactgc ctggtgctgt gcgataccaa
      841 cggaggcacc atgccggagg aaatcagctc cattatcagg acggtcaggg aacgcctgcc
      901 gcatgccctt ctgggcatcc acgcccacaa tgactgtgaa ctggccgtcg ccaacagcct
      961 ggccgcggta aacagcggag ccatccaggt gcaggggacc gtgaacggca tcggggagcg
     1021 ttgcggaaat gccaatcttt gttccgtcat ccccaacctc caggtgaaga tgaaaggctt
     1081 ttcctgcctc agcggtgcct ccctgacgcg gctcaaatcc actgccgcct ttgtctcgga
     1141 agtatccaat ctggcgcctt tccggcggca gcccttcgtg gggaacgccg ctttcgccca
     1201 caagggcgga gtgcatgtca gcgcgattat gaaggaagcc gctttgtacg agcacatcga
     1261 cccctccctg gtggggaacg cccagcgcgt gctgatgacg gagcagggcg gcaggagcaa
     1321 catcctttcc ctgtcccgca ccctgggttt tgaactggaa aagggagacc cccttctgga
     1381 cgtgctttcc gccgccgtga agaaaaatgc cgctctgggg tatgattacg tggccgcccc
     1441 ggccagtgcg gagctgctct tcctgcggca catgccggac aatgccttga aaccgtattt
     1501 caacatcctg cgcactgtgg tgctgacctc acgccatgaa atggacccgg atatgatggt
     1561 ggaagcctcc ctcaagctgg atgtccacgg caatgtggag cacaccgccg ccgggggctt
     1621 tggccccgtg catgcgctgg acagggctct gcgccgcgcc ctgacgcgct ggtatccgga
     1681 attggagcag atgcacctca tcgactacaa ggtgcgcgtg ctttccccca cccggacgaa
     1741 cattccggag gcggaggatg aaaacggcac cggctccaat gtccgcgtgc ttattgagtc
     1801 ctcggacggc gtcgccacct ggaccactgt gggcgtttcc tacaacatta ttgaggccag
     1861 cctggaagcc ctggcggacg ccgtcacgta caagctctac aagacggaac aggccagatg
     1921 gcgtgcggaa tgctgatggg cggcgctcct ttccggggga cgtgggcggt cctcatggct
     1981 tttccatttt cttcccgttc ccttgcgtgg ggcgggccgg aagccgaagg gcggaaaaag
     2041 ttcctcctgc cggttgaatg gctcaaaatg aacaggaaga agatcaatga gcttcgtgaa
     2101 caggcccggg cgacggtgtt cttttttgag acggctcctg tgttctttcc tgttctccat
     2161 tcatgttggt caatcacgtt gccgcagtta acgccgggac gccggattgc tgccagtttg
     2221 cccggagaac ggatgccctg ccggaaaaat accgttattt ttccggtttt tgcttcccgg
     2281 ccgcatctgc cggggtaaat aaaacattga cccgccagcg gggaagcttt atcctgaaat
     2341 cctcattcaa ccagaggtag attacagccg tggaggacga tattatttgt acagaaaaaa
     2401 tcattgcgga acgcaagacg ttctttcttg atctcaaaca gaatgcgcgc ggcaaagtgg
     2461 tgcgtattac ggaaaaggtg agctccaacc gggaccggat tatggtgccc gcggaaattt
     2521 tggatgattt catcgccgct ctccaggata tccgggagac actgaagcag cagggggaat
     2581 aaattttccc cctccctcct gttttttatt tcaccaatag cggggctgcg gccgtcccgc
     2641 catttcttag tatccgggtc attgcaaatc cggcgggcaa aaagccgttt tgtctgaatt
     2701 aggcgttgtc aaaaataaaa aagggagtat ttttacttcc cggttttgcc gtcccctttt
     2761 ttcatattcc ggagcattct gcatgccgga ggcacggggc cggaatgttg atgcaaccat
     2821 gaatatttca gatatatgac gacacaacag gaattgactc cggaattgat acgggctgcg
     2881 ctgaccacgg taaaatttcc ggggttcagc cgcgatatcg tatctttcgg gctggtgaaa
     2941 aagattgaca ttgacgctga aaacaacgtc acgattgatt tggtgattga gagcaagaat
     3001 gcggatattc cccgttacat ttttgaaggc gttcacggcg
//
LOCUS       DAWEMX010000204         3014 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10789_length_3014_cov_11.944238, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000204 DAWEMX010000000
VERSION     DAWEMX010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10789_length_3014_cov_11.944238"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          636 a          770 c          942 g          666 t
ORIGIN      
        1 gaataaagag tgtatccgag gatttgaagc gtgtctattc cgtttttgga aaacctcagc
       61 cggaacattt tccttctggc ggtcttgaac ttccagaagc attgaaggag gcgcaggtaa
      121 atccaccgtc cggccagggc ggcctcaaga taagccctct gttccctggg catcccgcaa
      181 acgtgttcgg aagtgatgtc cgcctcccgc cagagttccg cccaatgcct gagccgcccg
      241 gccggcattt cattgtccgg aaaaagcagg gggacaatat aattcatgta ggccgtgcgg
      301 ttgctgtcca ggaccccttc ccggagtttt tccctggtga gcatttcgaa tacggcgtaa
      361 taacttgcgg gccgggtttc cgctttccgg ttgcgagaag aaacggattg ggggccgccc
      421 agcccgtagt ggtagagttt ttcagaggtg agggcgacgc tgcggcagcg ggcgatggcc
      481 cgggaaacgt aaagggtgtc ttctccgctg gtgaaggaag aaggcaggcg gacttccagg
      541 ggggcggaac agccggggag tatccgcctg aacaatttgc tccagacgac gccggtgatc
      601 ctgtcttcca gaaagagcct gaatatttct cctttctccg taaagacgcc gtcgcaggcg
      661 gtgttttcaa tatgaaggag tttttcctca gccattccgc gcgattctcc gctttcagcc
      721 ggaaggaagg gggaatagga acattggacc atgtctgccc cgctttcttc cgcgagccgg
      781 tagagccgtt cgtacatgtc cggctcaacg tagtcatccg gatccgcaaa acccaggtaa
      841 ggcgccgcgg ctgccctgat tcccgcatta cgggcttccg ccctgccgga attttcctgt
      901 gtaatcaggc ggaaacgact gtctccggaa gcatatttcc ggacgatgcc tccggacccg
      961 tcggttgaac cgtcgtccac gcagatgatt tccaggtcgc ggagggtttg tgccgccagg
     1021 ctgtccagac agcgcggaag aagggactcc atgttgtaga tggggacgat gacggaaatg
     1081 aggggagagg gcatggtctg ctgtatagga aacggggtta tatccggagc catgagccgg
     1141 ggatgagcgc gtcatccgta tggtaccggt cgccttcctc cctgttgaac cagatgcgcg
     1201 gcgcgatgac gattttctcc gcgtgttcgt tgagccaggc cgcccaccag gagaaagtgc
     1261 tgttggcgat gatgtggtgg cggcagtttc gcatcagttc cagatcgaag tagccggtgc
     1321 ctccgtcatt gatatccaca tggacatggg gcagggcggg cctcaggttt tgccgggccc
     1381 attctatgtc gtccgagaag atgaagtaac gcagggattc ctgcggagcg cctgccgccc
     1441 gcagccttcc ttccatttca gccatgctcc gtagatagta ttccagaggc ggaggagaaa
     1501 ggtaggggtt ggagaggtaa tccgttctgc ggatgtgcag ggaaatggag cagcatgaac
     1561 ggatatcttc aagaatgcgg gcgttttccg gagtcagggg agttttcaga cggaattccc
     1621 tgcaaagctg ttcccggcag tggaggaagt attgttccgt ctgaaaatat cccttgaaat
     1681 aggtatggcg gcggggagga gccgcgagac cgggatcgaa cccgtgccgt ttttcctccc
     1741 ggaaatgagg atatttggga gcggcgaaca gggaacgcag gcaggcggga atacgggact
     1801 tgcggaaagc ccagggggga gggcctcctt caatgggaag ggaaacccgg aagtgatgga
     1861 gttcgcagac cctcttatca tcatgttcat atgatgaggc ttccagtctt gcttttcccc
     1921 ctttccggga gagcgcgaag aggaaggcgt attggaacag ctggtttccc agccctccga
     1981 aaagacgcat gactattatt tttccttttg ccatgttcaa gcaggggaag gggttaaaac
     2041 gtgatattcc tgcgtatttc ctccagctgt tcgcgtactt gccgcagccg tgcccggcca
     2101 ttcctcctcc atgaggggac agggcagaaa tggaggagct tccggcggat tccggtgttt
     2161 ttgccggccc tccggagcct tcttccgaag tctgaaaagc cggaggattg caacaatagg
     2221 cggaaaggaa gatgcaggga ggccctccgg cactcggcgg ccgtgcaatg ggcgttcagg
     2281 aagtcccgga tgatgctgta aagtatttcc tgctccggcg gggagaaagg catgccaagc
     2341 agcctcagtt caaatggttc atgctcaata aagcggacga gatggccggc ttttcttttc
     2401 cagagatggc gttgttcata aaaatcttca atcagggaaa gccaccgggg gatcatgggc
     2461 aggatgtcgg cggttctttt ccgtatctgg tggtggttgc cctgttcatg ctggcggtaa
     2521 tggtaaacgg cctccggaca gaaggaaatt ttttctgtca gcgcgagcac gaaatgcgag
     2581 aagaggccgt cttcacaggg gtgaatgcct tccgggaatt ccagattgtt ttttaagggg
     2641 aactcccggc gcagaaatat gccgcaggtg ggcagggcgg ggattttgtc cggcggaaac
     2701 ctgtcgcaga acagctttcc cgctacggtc aaatcgcttg ctccgcggtc cgaggcgtcc
     2761 agaagatgtt tgagaaaggc cgcgtcgatg aaatcgtcac cgtcaacaaa acataaatag
     2821 cgcccggaag cgtgccgcat gcccagattg cgggcggagg aaacgcccct gttttcctgt
     2881 tcgaggcagc ggattcttga gtcctcctct gaaaagcgcc gggcgatttc ccctgacgag
     2941 tcggaggacc cgtcgttgac gatgatgcat tcccactccg caagggtttg ccggcggatg
     3001 ctgctgatgc actc
//
LOCUS       DAWEMX010000205         3002 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10837_length_3002_cov_10.477435, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000205 DAWEMX010000000
VERSION     DAWEMX010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10837_length_3002_cov_10.477435"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          776 a          850 c          772 g          604 t
ORIGIN      
        1 atatggttta ttgcggctcc cgcatttgcg ggcgaacctt aacagtgata gcaatggcat
       61 acgcaatttt caaaacaggt ggcaagcagt accgcgtcca gacgggcgac gtgatcgacg
      121 ttgaatgcat caataccctc gaagaggggg ccgaagcaag cttcgatcag gtgcttatgg
      181 tggaaaatga cggaaaagtg accgtcggca cccccatggt ggaaggagct acggtctccg
      241 ccaaggtggt cagccagttc cgcggcaaaa agatcatcgc tttcaaattt aagcgccgta
      301 agggcttcca caagaaaaaa ggttcccgcc gcgccatgac gaagctggaa atcactgcta
      361 tcaacgccta atttcaaccc ttaattttta tcatcttatg gctcacaaga aaggtcaagg
      421 ttctgtcaag aacggtcgcg actcccgcag caagcgcctc ggcgttaaaa aattcggcgg
      481 acaggaagtg atcgcgggca acatcatcat ccgccagcgc ggcaccaagt ggcaccccgg
      541 caagggcgta ggcatgggtc gtgactacac catcttctcc ctggtggacg gccgcgtgtt
      601 ctttgaccgc gaaggccgcc gcgtcaatgt ggctccggaa ggatctgaag ccgccaacta
      661 aaatacggat tactctatct ccctgcaggg gcggccatcc acagggatga ccgccccttt
      721 ttctgtcctt ctccgcgctc caataaataa caggaattgt tcttgcaaaa caatagcccc
      781 tcccccacta tccacgcaga gcatgaaaaa taagatatga atcccaaaac agattccaag
      841 caggccgtcc gcactgcctc taccctgccg gtcatttccg ggttgaggct taccaagcaa
      901 aggcaggaag tttatcaggt tcttctggaa caaagggatc atccaacagc caatgaagtt
      961 taccagcggg tacgcaaaaa actccccagc atctccctgg ccaccgttta taactgcctg
     1021 gaggcgctgg tgaaccacgg cctggtcaat caggtcaact ttgaacgcag ttcttcccgt
     1081 ttctgtccca atctcgttga gcacggacac ttccaggaca cccggaccgg acgcatttac
     1141 gacatcacca tcaaggaagg cgtggacctg aaggaattca tcaacttgcc cgacgacgtg
     1201 tgcgtggaag aagcccaaat caccctgagg ggttccatca ccacgccgca gggctaactc
     1261 tctttcatca tcatgagtct ggtcattaaa aatttgcacg ccaacgtgca aggcacggaa
     1321 atacttaaag gcatcaatct ggaaattccc aagggagagg tgcatgcgat catggggccc
     1381 aacggctccg ggaaaagcac tttatccaaa gttctttgcg gacatccgga ctatgaggta
     1441 acggagggag aagcctggct ggacggacag aacctgctgg acatgagcat tgacgaacgc
     1501 agcagggcgg gactcttcct ggcattccag taccccatgg aagttcccgg cgtttccaac
     1561 gccaacttcc ttcgtgcggc catgcaggcc cgcatgcccc atggggagga actggatgcg
     1621 gtcaccttct acaaaacgct ctatgcaaaa atggaccagc tcggcatggc ccgcaaattc
     1681 acgtcacgcg ccgttaacga aggtttctcc gggggagaga aaaagcgcaa tgaagtgctg
     1741 caaatgcttc tcctggatcc cctctatgcc attctggacg aaacggattc cggcctggac
     1801 atagatgccc tccgcattgt ctcggaagga gtcaactcca tgcgttcccc tttccggagc
     1861 ttcctggtca tcacccacta caaacgcctg ctggactata tcaggccgga tgtggtccat
     1921 gtactggctg acgggcggat tgtccggacc ggaggcggag aactggtgga cgaactggaa
     1981 agcaggggat acgagttcct gaaaaaaacg gaaacagcgg aaaaggcata acgccgccaa
     2041 tccggcaagc aatcatgagc gatacacctg acatgtcaga gaacgggaac acacagaacc
     2101 tgtttgactt cgaccggagc aaggggaact tctccttccc ggaacgccat aaatttgatg
     2161 ccggatacgg cctgacggag gccactattg attacatctg cgacgtcaag gacgacccgg
     2221 aatgggtgcg ccagttccgc aaaaacgcgc ttgccgtttt tgaaagcaag cccatgccca
     2281 cccactgggc ttctccggat attaaaaaaa tagacttctc ccaaatccgc tactacctct
     2341 ccgacggaga acggcccaaa cgaagctggg acgacgtgcc ggaggatgta aagcgcacct
     2401 tcgaacgcct gggcgttccg gaacaggaac gccagtttct ggcgggcgtg gaagcccagt
     2461 acgactccga atccgcctac tccaacatga aggaggaact ccgcagacag ggcgtcatct
     2521 tcgtcaactc cacggaaggc ctgaagtacc atgaagatgt cttccgcccc tggtttggaa
     2581 aagtcattcc cacgggagac aacaaattct ctgctctgaa cagcgcagta ttttctggag
     2641 gttccttcat ttacgtaccc aaaggggtta aactcaaaca tcctctccag gcgtatttcc
     2701 gaatcaactc ggaaaatttc gggcagttcg aacgaactct catcattgcg gatgaaggtg
     2761 cggaactcat gtacatggaa ggatgcacgg caccccagtt tgaaacttcc accctccact
     2821 ccgccgttgt ggaactggtc gcgctcaagg gcgcgaaaat tcagtacgtc accgtgcaaa
     2881 actggtcctc caatgtcttc aacatggtca ccaagcgcgg ccttgccatg gaagatgcgg
     2941 aaatccgctg gatagactgc aacattggtt ccggcctcac catgaaatac ccggctgtgg
     3001 tg
//
LOCUS       DAWEMX010000206         2977 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10938_length_2977_cov_11.541410, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000206 DAWEMX010000000
VERSION     DAWEMX010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_2977_cov_11.541410"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          702 a          889 c          777 g          609 t
ORIGIN      
        1 cctgaatccc cagacggtgg aagacctgcg cttcgccctc ggccaggaca tccatgttct
       61 ggtagcgccg gattaccaga tttccgaacg catcaatgaa ctctatggag gcgaatccgc
      121 cgccatgtcc gacctgatgc aggaactgaa caacatgcag gtcaacaatg agacggagga
      181 ttccgccgcc gctcccgtca tccgctttgt ggacctcgtc attacgcagg ccatcaagga
      241 aaaggcctcc gacattcact tcgaaccttt tgagaaggaa ttcaaaatcc gctaccgtgt
      301 ggacggcgcc ctgtatgaaa tgcagcctcc ccccgtccac ctgtccgtgc cggtcatttc
      361 ccgcgtcaag gtcatggcga acatgaacat cgcggaacgc cgcattccgc aggacggccg
      421 catcgtcaag cagataggaa accgttccgt ggacatgcgc gtttcctccc ttcccactca
      481 gtacggagaa tccgtggtgc tccgcgttct ggatcgctct tccgtcaact tgaacatgga
      541 caacctgggg cttcccgcgc atatccacga atatattctg gatacggtcc acaagcccaa
      601 cggcattttc atcgttaccg gccccaccgg cgccggcaag acaactacgc tgtatgccgc
      661 cctgcgtgaa atcaatacca ttgattccaa ggtgctgacg gcggaagacc ctgttgaata
      721 cgatattgac ggcatcatcc agattcctat caatgaagcc atcggcctgg acttcccaat
      781 ggtgctccgc gccttcctgc gacaggaccc ggaccgtatt ctggtggggg aaatgcgaga
      841 catggcaaca gcgcagatcg ccatccaggc atccctgacg ggtcacctgg ttctctccac
      901 cctgcacacg aacgactccg ccggagccat tacgcgactg gtggacatgg gatgcgaacc
      961 tttcctggtg gcggcttccc tggaaggggt gcttgcacag cgcctggtgc gcaccatctg
     1021 tccggactgc cgcacgccgt atgaaccctc atccaccatc ctctcccagc ttggcgtctc
     1081 tccctatgaa ctgggagaca agcacttttt cacgggccga ggctgtgata aatgctccaa
     1141 ttccggctac aggggccgca aggggattta tgagctcctg gatattaacg ataccctgcg
     1201 cgacatgatt acggaccgcg ctccttccgt ggtgctgaag cagaaagcca ttgaaatggg
     1261 catgtccacg ctgcgggaag acgggctgag aaatatttat gacggcaaca ccaccattga
     1321 agaagtgctg aaatatactt aacggacagc cggacgccgt tgcttcccct tatttcaacc
     1381 attaagaaaa aacacttaaa atacaacata tatgcctaaa tatcaataca cagcacttga
     1441 ccataaaggc gaccagaaaa caggtaccct ggaggccaat tccgaagcgg aggccatgga
     1501 atccatccgg gcgcatggcc tgtaccccac ccagatcgta gaagcgggca agggcaagat
     1561 tcagcagacg cctgccgcca agaaaaaggc caagggagcc aagaagcaaa aaggcaagct
     1621 gggaggcaaa atcaaggcca aggctctgat gattttcacc cgccagcttg ctacgctgat
     1681 tgacgcgggg cttcccctgc tccagagttt gaacgtgctg gccaaacagg aggcaaaccc
     1741 caacctgcgc gtaaccattg aggctcttgg agattccgtt cagggcggct caaccttctc
     1801 ggaagccctg gcccaacacc ccagaatttt tgaccgcctg tttgtcaaca tggtaaaggc
     1861 cggggaactg ggcggtgtgc tggaagtcgt gctgaaccgt ctggcggaat accaggaaaa
     1921 ggcccaaaag ctgaaaagca aggtaatctc cgccatggtg tatccctcca tcgtcctgtt
     1981 tatcgccgta ggcatcgtga tcttcctgat gctggtcatc gtgcccaaat tcaaggcgat
     2041 gttcgcagaa cagaaccagg aacttcccgg tatttccgag tttgtgttcg gcatcagcga
     2101 ctggttcatg gccgccccct tctttgtgcc caatgccgtc attctggccg cggtggtcgc
     2161 catcctgtac gctgttttca cggccatgag caagacgccc aacggacgcc gcaagattga
     2221 ctccgctctg ctgaccatgc cggtcatcgg caatgtgcag agcaaaagcg ccatcgcccg
     2281 cttcgcccga accttcggta cgctggtgac ttccggcgtt cccatcctcc aggcgcttac
     2341 catcacgaag gataccgccg gcaacatgat cgtgggagac gccatcggcc tcatccatga
     2401 ttccgtcaag gaaggcgaat ccgtagttac gcccatgtcc tcctccaagc ttttcccgcc
     2461 catggtaatc tccatggtgg acgtggggga agaaaccggc cagctgccgg acatgctcct
     2521 gaaaatcgcg gacgtgtatg atgatgaagt ggacaatgcc gtgggagcta tgacctccat
     2581 gctggaaccc atcatgatcg tattcctggc cgtggtcgtg ggcggcatcg tgttcgccat
     2641 gttccttcct ctcctgcagg ttattgaaaa gatggggtaa cccctcaagc agaatcagat
     2701 gaaaaatcat tccgcatacc gtcggctccg ggaggcaaag gggttcaccc tcatggaaat
     2761 cctggtagtg atggtgatca ttgtggtgct ggccacgctg accatctcca tttacacctg
     2821 gcttgaaacc agaaagaacg aacaggctac ggaatccatt gtccgcaaaa ttgaaatggg
     2881 gctggagagc taccacagcg accatgaccg ctacccctac ggcacggaag ctcccttcaa
     2941 caatcacggc gtagccgtgg ccaacgggga ggaatac
//
LOCUS       DAWEMX010000207         2967 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_10968_length_2967_cov_9.131525, whole genome shotgun sequence.
ACCESSION   DAWEMX010000207 DAWEMX010000000
VERSION     DAWEMX010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10968_length_2967_cov_9.131525"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          713 a          692 c          742 g          820 t
ORIGIN      
        1 aacttctttt gatacacagg gattggattc tttcatggaa aaccagatct ctttcggaaa
       61 ctcttggcag acctttatct ccggccccaa aggagcttct ctttccctga ctatttccgg
      121 tctggtggct ggtgcttcct accagattgc ccttgttacc gggtgtcctt ttgagggggc
      181 cggtgcctgg aattccctgg tgacttccaa tacctacgac agcgcggctc cttccatagg
      241 tgcgaactct cagacaattc aggcccggat tcccacgggg tacaccatta gtgacgttat
      301 tgccaatgaa gatggcgaaa ttattctgac ggtgaatact ccgggttcct cccacacgcc
      361 tacgttcaat gctctggcta tttctggaga aacagccagc gttccggaac cggccacggc
      421 gtccctgagt ctgctggggc tgacggccct gatggctcgc cgccgaagag cttgagaacc
      481 tcttttccgg cagtaaaaaa tgttgttgcc tgcaaggtct agccttgcag gcaatttttt
      541 gtaatcatcc gatttgagaa aactgggccg tccgaataac cgtttttccc tggcggaact
      601 cttgctccgg aggaaatgag gctcctgctt ctttgacatt gcgggaacgt tcttcatccg
      661 ttactctttg ctctgccgta gagagaaatg atcatcattg cgtggaacgc aacagggaat
      721 tgattccgcc ggatacgggt atgttccggg tcatggacgg ggcgggggat ggatctttgt
      781 ggatcagatg gcggatcgtc ttctcgtctc tacgcgcggc tgttccattc ctgcggacct
      841 ggaaagcgaa ctccgggata cgggcatgcc cgtattccac aaaaaactgg aacaaaacca
      901 gaaagaaacg ttctgaacat cattggtctt ctgtggcagc gttattcccg tccgggacta
      961 tgacttcctg aaggaacacg gagctgtggc catcttcgga ccgggcacca acattccgga
     1021 agcttccagg gaaatcatgg aagctcttaa cgaacagtat gctgaataag cggaaatcgt
     1081 tgttggtcag tgtaatgacc tgtgagaagc cgaatgaaaa atccggcctc tttttttgtg
     1141 agcggattaa ctatccgctg tcaaactatt tttttaaaaa aaaggaattt acgcagattc
     1201 acagacagaa aaaacagatt ggcaaatcgt agaaaatatc tgtgaatgtt ttttggacga
     1261 gtttatttca atttccatga tatataatat gattttcaat taattacgat atttgttcat
     1321 cattttttga gaacattgtg caatacctgt tttttcccaa cggatagtta atccgcacaa
     1381 tgtcgcatat gaagttttca acttttatct ctctcttatt gggggggctt cttcaaggca
     1441 gtgctgttgc cgctactaca ttttttacgg atctgtcgca gaatttttct ggtggggata
     1501 tctcctactc ttctccggaa gatgacagcg tcaacgggtt tgctgcaggt gccatttctt
     1561 ccgcagttac caccggtggg gagcgtcatc cttcctccat cacgctcagc ctgaatctct
     1621 cggctatgcg gagcgccata caggaagcag attttttcaa cattttgctg gttgatttaa
     1681 gaataagcga aaactcaact tccggttcca taggcttgat gttgacggcg gatggtctta
     1741 agtttgccta ccagggatct gtgtggagtc ctggtgcttc tgcttacctt tctctggaga
     1801 gtttggcgaa tctttcctac accgtggaag agactgatta cgtcaccctt actctcgtgg
     1861 cggctgtcca gtcagacgga gcaaagggaa caaaagtagt tgatgacaag ggtaatggag
     1921 tgccatgcaa taatggtcgg aattttgcct acaccgattt gacgacatcc caaaactatc
     1981 aaactgtgct tgttaacacg gactatgtaa cgggcatggg cattacgccg ggcgtagcga
     2041 cccctgaaga aattgttgct gcaaccagga gtcttgccgt gccggaacct gctacggcgt
     2101 ccctgagtct gctggggctg gcggccctga tggttcgccg ccgaagagct tgagaacctc
     2161 ttttccggca gtaaaaaatg ttgttgcctg caaggtctag ccttgcaggc aattttttgt
     2221 aatcatccga tttgagaaaa ctgggccgtc cgaataaccg tttttccctg gcggaactct
     2281 tgctccggag gaaatgaggc tcctgcttct ttgacattgc gggaacgttc ttcatccgtt
     2341 actctttgct ctgccgtaga gagaaatgat catcattgcg tggaacgcaa cagggaattg
     2401 attccgccgg atacgggtat gttccgggtc atggacgggg cgggggatgg atctttgtgg
     2461 atcagatggc ggatcgtctt ctcgtctcta cgcgcggctg ttccattcct gcggacctgg
     2521 aaagcgaact ccgggatacg ggcatgcccg tattccacaa aaaactggaa caaaaccaga
     2581 aagaaacgtt ctgaacatca ttggtcttct gtggcagcgt tattcccgtc cgggactatg
     2641 acttcctgaa ggaacacgga gctgtggcca tcttcggacc gggcaccaac attccggaag
     2701 cttccaggga aatcatggaa gctcttaacg aacagtatgc tgaataagcg gaaatcgttg
     2761 ttggtcagtg taatgacctg tgagaagccg aatgaaaaat ccggcctctt tttttgtgag
     2821 cggattaact atccgctgtc aaactatttt tttaaaaaaa aggaatttac gcagattcac
     2881 agacagaaaa aacagattgg caaatcgtag aaaatatctg tgaatgtttt ttggacgagt
     2941 ttatttcaat ttccatgata tataata
//
LOCUS       DAWEMX010000208         2910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_11211_length_2910_cov_8.438529, whole genome shotgun sequence.
ACCESSION   DAWEMX010000208 DAWEMX010000000
VERSION     DAWEMX010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_2910_cov_8.438529"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          906 c          737 g          580 t
ORIGIN      
        1 ttgtcatcac aaccgacacg ccggatgagg acatctggct gaaggacggg gatccggaca
       61 gccccttttt ccatacgccc cgttccgccg ccaatacgga aattcgtgaa atccccacgt
      121 cttccgattc ctggcccgcc tggaatgaag aggaagtcaa cctgcacatg aatcttccgg
      181 aatcggcgcc gcgccccctg gtagtgcagc cggctccgtc cggagccatc cgcaccatca
      241 tcaaatcctt taacggaacc gtttttgagg cggatgccca gcccatgcat cccgtctggg
      301 atcagctccc gcccggatac gttcctatcc cggctcttcc ggcctatcag cccaccttat
      361 tgaagaaatt acaataggag ggggcaaaag cctcagaact tgaagcggac agccccgaaa
      421 acagaccatc cattgaaagc tttggccgtt gccgaatcca catggctggt gacatactct
      481 acagccagca tcagcttgac cgcattgacg gcctccggac acagatagca attcagcccc
      541 aagtaaaagg aatgcatgct gtccacccat gcgggataat gggtgacgct gggcacataa
      601 cgggtattca gcttcacaga acggtttcca aaggaacact ggtattgaaa gacgccctca
      661 aaatgcgggg aaatccggta aacgggctgc agaaccaggc cgtacacatt ctttgcaccg
      721 ggctggccta caattcccac gcccgccagc aaattcccca tgatggagaa agcgccgcgg
      781 ctggcgtccc agctcaggga aatcacgtcc tgcgcccccg ttccgcagta atcggacgtg
      841 gaaggaattt tccttccccg ccattccgta aaattatggg catactggta ccccagaaac
      901 tggctgtccc acatgggaga agccaccttc cacttcatgg cgttcaggat aaacaggttg
      961 tctgcagaat gaaactggat ctcatccttc aaatccgtac cgttcgcatt caggtaaatg
     1021 ccgtaggaat ggtacaggct cttggcatcc ttctgaaaat tggcctccag accccagttg
     1081 gaaatgggaa tcaattcatt gcacagggcg gatctttcca ctgttttaat ccgggaggaa
     1141 gccaggcaat actccgacgt caggtgaggc gtcagcttac cggccctgaa cttgacgccg
     1201 ggcatggtct tttccagata aagttcatac agggaccact ccgtatggct gccgatccat
     1261 tctccccgca cttcacggtg ccggccttcc aaatccccca cattcgtcag attggacagg
     1321 cgccaggaac cgtcccccat caggatgtcg ccgcccaggt aagcgcgcct ccacccgttg
     1381 ttatggccgc cggaagacgg gcagaattta ttggccccat tggggctgac ggctgctgcc
     1441 tgatactggc caatcagcgt cagcttcacc ttcctgatcc atgaggaatc attccggtac
     1501 agcaccggac cgcgatccag cacacggcac agggacggca gagagccccc ggaaaccggc
     1561 tgggactgaa ctgctcccgc gccagcttcc gccgccggaa aggcggcata ccaacaagcg
     1621 gccaacagga acattcctgg aaaaacaagc ttcatcatag gctaaacaaa caaattgggc
     1681 gcacatctta aggagccctc tccgaaaatc aagcccggat gctcctttcg acattgttgt
     1741 tttctgaaag ggttgttacg aagaacggca atgatggacg caccagtgcg cccatccgga
     1801 acgtctccat cagaaggcca gggacgcagg tttcagcacg gcttggagtt acggctacat
     1861 cggcctgttg tatctccggc ctgatgaacc gggccatttc aatcacttcc tgtacgatga
     1921 agaaacaacg atgcagcagc ctggcatcat cgcgaacagt taccggcacc aaatcatcac
     1981 tccggcaagc tttccctccc ggcccgcatg ggggaacatg cctgaataat tatttcccgc
     2041 tcctgcgggg aaaggccgta aatgtcttcc atcagctcat tcatccgctc atccccttcc
     2101 gggcaatagc ggaccgcgag agcgtccgcc agcccttcca cttcccgcag cagggacaga
     2161 ccggggaccc ggatgggagc ccggaggaca ggggcggtat ccatctggaa aaaatccccc
     2221 tgcatttttc cccggcggag aaaccaaaac tgcatcaggc gcgaattgaa cagggccgcc
     2281 agatatttca tgctcacgcg ttccgagcgt atcacgttga atgccatcat cacataagct
     2341 tccttttccg tataggagaa ggttggacgg gcacacttcc tgacagccag aattttgggg
     2401 ccgggccgga aaaaacgttc ctgccggggc cagtgcacat ggtaatattt catgcggccc
     2461 atgcgtgttt cccgccgggc ctccatcagg ggacggaatt tttccaaatg gcgcaaaagt
     2521 gtaacggcct gctccgctcc gttggacggg gtcagatata acagcacctt ctccggagct
     2581 ttcagtgcat gccgccctgc ctggacaggt tcatacagag gcttgagaaa acgccgctcc
     2641 cgttccggca acaggccaaa atacccctga ggcacgacaa ataccccatc ccccctccgg
     2701 attccgtacc gccgcactgt ttccggcgcc agattctcca gagcgcggga agaaacgacg
     2761 tcaggattcg gcacaatccc ctgcgtcatt tccttggcgg catccagcct gaaattccgg
     2821 catgcctcca tcttttccag aagatgccgc tctgctgcag agcagaagga caatccttcc
     2881 ctgacgcacg ctccggcatc ttccggcaag
//
LOCUS       DAWEMX010000209         2874 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_11368_length_2874_cov_7.778645, whole genome shotgun sequence.
ACCESSION   DAWEMX010000209 DAWEMX010000000
VERSION     DAWEMX010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11368_length_2874_cov_7.778645"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    624..633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          735 a          778 c          681 g          670 t
ORIGIN      
        1 atatatacaa aattaatgat ttggaattgt ttatgaaaat catgtcgata tcagattgcg
       61 aattgctcaa aaaaatagat atgttagaaa aaaagattgg ttctttacaa attaatcatc
      121 tggaacggac gacaatcaaa catttgataa aagaaattca aaacaaatat aaaatacatg
      181 aaaatattta taatgaatta agaaaaaaaa attaaacagt aacatagatt gattcgtatc
      241 aagaagaatt tgtatatgat aaaattgctt ctccgagaga aatcatgctt gtctccagaa
      301 gcctgagcta tgatggcctg gatgccgtcc cctcctacgg ttacagctgc gacaacatcg
      361 gtaaccgcaa gacggcacag gaattggcct aagaactctc ctacgcagcc aaccgcgcgg
      421 cgagcttcac cagccgggac ggagcgacca tcttggaatg cggctacgac cgccaggaac
      481 gaaggtacat gaagaaaatc accgtcaacg ggacagtcgc cagccatgga cgaaactgta
      541 ccaggactat ttacagatag ccgcctcttg actgccggac gccgaacaaa taaaatgaat
      601 aaagagaacc ctatatttgt tttnnnnnnn nnnaatacca tgcttgattt tctcgttttc
      661 ctgctggata tcccgtccct tcttgctgat ttttctcctc gcgccaagca taagggagaa
      721 tcaccgtact ggcgtttcat ggggcttttt ttactgactg cagaactggt tattctattt
      781 ttatgggata ttacggaaag cccgccagct cttgtagcct gtattacttt gggcctctgg
      841 ctgatcgcgc acctctccat ccgcgccttc aggaacggga aaaacgtaaa cggcctctcc
      901 gtccgttccg ctccgccatt ttcctcacag gcgccacaaa gaaatgaagc ccctaccccg
      961 tatctccctg aaagggaata gctatataat ttatttatta tcaatcattt taaaatatat
     1021 acccggattt ttgtcataca aatttcttct ttttgaacgg atgctcctgt ccgggactct
     1081 aacctgcatc agcaacaaac cgggcggcgg gaggtagaga gaccgccgcc cattatgggg
     1141 agttctgagt ttcataggta atatagttgg ataaaaaacc cgcacggggg aaaccctgtg
     1201 cgggtttatt catcccccct cccggaatgc cggaaaacag ggccggaaca ggccgcagaa
     1261 aatcatggct gaaccaaatt aaggcttgag agttgaaggc atctcgctaa tctggcttca
     1321 gcaagaatca tctgttatca tggtcaccac gaacgaaccg aatatttacg aaacgggagt
     1381 actggactcc aatgcggaag gcaattttgt ctataccacc ctggacgccg ccatcaactg
     1441 gattcgtaaa aattcccttt ggcccatgcc gatgggtctt tcctgctgcg ccattgagtt
     1501 catggccgtg gcctgttccc ggtatgacct ttcccgcttc ggctcggaag tgacacgttt
     1561 ttccccccgc caggcggacg tgatgattgt agccggcacc gtcacttata aaatggccct
     1621 ggccgtccgc cgcatctggg accagatgcc ggaacccaag tggtgcatcg ccatgggcgc
     1681 gtgcgcttcc accggcggca tgttccgctc ctattccgta ctccaaggcg tggacaagat
     1741 tcttcccgtg gacgtttaca tttccggttg cccgccccgt ccggaggcta ttctggaagc
     1801 cctgctcacc ctgcgcaaga agctggatac ccagcagccc gcacgcactt tcttcaagaa
     1861 ggaagaacct cgcgaagcca acgctcccgt cccggtcaac acggaaatgc cccttgaata
     1921 agttttttca aacgtttctt ccgttcactt ttaccatttt ttctccatgc tttctctaga
     1981 atcacagatt aaaaccgccg cagacaacgc ccaaaaccgc tttgggaaag acatcatcac
     2041 ggaccgctac gaattccgcg gagatattac gctgaccgtc gcgcgccaag ccgtggcgga
     2101 tgttgtccgc tggctacggg attccgccgg gtttgatatg ctggttaacc tttcttccgt
     2161 ggacaatatg ggggaaagcc cccgctttga agtcaactac acgctgacac agtcggaaac
     2221 gggagcgaac ctgagcctca agacgaaggt ggacgatggc gaagaagtgc ccagcgtcac
     2281 ggaaattttc cagtccgcca actggcacga acgtgaagca tgggacctga tgggcatcaa
     2341 gttctcaggt catcctgatt tgcgccgcat tttgatgtgg gaaggttatc cttatttccc
     2401 tctgcgaaag gatttccctg ttcaaggcgt acctactgaa ctgccgggcg tcgcctttac
     2461 ggaggcggct cccacccagg gagcgccgtt cgctacggaa caaggccgct gccccagtgc
     2521 ctgccgcgaa ccccggtccc ataaattcta gcccattttc cagccccgca aggcggggtc
     2581 ccacccatga cccgactcat cctgcccata tgcatcatca gcctgctcag cggctgccag
     2641 gacgccaacc cggcagagcg ggaatggaag gagcagttat ataaaaacct ggcaatcgtg
     2701 ggggccagga actggattgt catcgcggaa tcctcctttc ccgcctacac gggaaccggc
     2761 atcagaacca tggtttccga caagacgtcg gacgaagtac tcctggatgt gctgaatatg
     2821 ctggaagagg aagcccacgt ggtgccccgc atcatgatca gctcggagct gcgc
//
LOCUS       DAWEMX010000210         2853 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_11461_length_2853_cov_10.572552, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000210 DAWEMX010000000
VERSION     DAWEMX010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11461_length_2853_cov_10.572552"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2210..2273
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          692 a          711 c          672 g          714 t
ORIGIN      
        1 ggatattcct tctttgtttt catctccccc atatgtcctg aaaaggcaat tccatgtaaa
       61 ttcattagga tacgaatgtt tatagacagg atgaccatcg gcatcataac cctgaatttc
      121 atcccattgc atatggccat tcaccccgga ttttatacct atgttgtcat gcaatccaaa
      181 acggttgtca agacctacgc tccaaacgct gtccgctgca tggggctgcg ccagacttcc
      241 ggcagctcca acatacccct tgtctttttc aatgacacga attcctttaa aacagacatc
      301 cagaggatgg atggaaacga gaagctgtgc ggaaactcca tgtgcaggat ctccatcccg
      361 ggggaatccc atatctgccg caggaacgcc tttaaccatt cctcccaaag tttcatgttc
      421 cgcagtaaga tgttcaggaa aaacaacgtc aaattcgata gggcggggga gggccgttcc
      481 cagttcggtt tccacttcaa tggctacttt ccctccagac tgctggattt gttctttact
      541 ggcacaagga tttatccgaa atttagcttt ttctgttgtt tcttctgttt ttaagccatc
      601 ttcaagtaac tcttttccct caatcacttt ccattgaatt tgatccttcg gctccaacag
      661 aaacggcttt ttacagttca gggtaacggt gaccgtttct ccaatgccga ttttcctgcg
      721 ctttctgtct tccttcttct gttcttccgt cgcgtttttg tcacgggcca gtttggcgga
      781 acagttctcc gtttccgata ccagttccat gatatcggaa aattcctcat cttcggcatg
      841 aagaagggaa aaagatgaaa aaaggatgaa aatggcaacg gtacggacaa ggcaagggcc
      901 actcatttaa tgaagctatg aaatgcttgc atatttgtca agtgacagag cgcgttttcc
      961 gtccaatgag aatgggagaa tgctgcctaa actatacttc gcataatata tgtaatgcca
     1021 aatataattc cggtagaata attctccgac cgttaatatt taaatgatta tgatatgatt
     1081 gggaggacgt gaaaaaatgc ggcatgggaa tgaatgatga actatcctct gggatgaaag
     1141 cggtgatgaa tcgagttcag ccgctgctgc tccaaatgcg tatagatctg ggtggttgaa
     1201 atatcggcat ggcccagcat ctcctgaata atacgcagat ccgctccgtt ttccagaaga
     1261 tgcgtggcga aggaatgacg caggatatgc gggaaaacat gctgttccag tcctgcggct
     1321 ctggcgcgtt cccggagaat ttgacgaatc cgctccctcg tcaacggacg gccgcgtact
     1381 gtcagaaaga tgtggctttt cgttcccgtc ctgaccagtt tgggtcttcc tttttccaaa
     1441 tacatttgca aagcatccaa agcggatctc cctacgggca ccagccgggt tttattcccc
     1501 ttccccagca cgcggacaaa agcttcatct ttatcaaaac tctctaattt gcaattaatt
     1561 atttcactaa cacgcatccc gcaggcgtaa atcatctcca gaagcgctct gtctctacat
     1621 cccagaggaa tatcttgaat gtcaatgctt tcaagcaaat ttgagacggt tttctgatcc
     1681 agcacatgag gaagcgacaa cccctgccgg ggcatttcca gaaatacgga agggtctttt
     1741 tccagtattc ccgttccggc cagccaccgg aaaaaaatac gcagatgcac catttccacc
     1801 cgcatggagc tgcgggccat gccccggtcc tgcaaatgcc gcagaaatgc cgaaagcgtg
     1861 tcgatgcccg tttgggcaag gccggtatta tccatttcca gaaaccgggc aaattcctcc
     1921 agggtctgtc tcacggaaag ctggtaggcg gtgctcaggc ctttctccgc ggccagaaaa
     1981 cggatgaagc gttccaaatg agtttgcatg gaggctgctt ttcctgtcat ctgttaaaac
     2041 cgttccacca ggaacaggaa ttccgtcacg ttctgaggac ggtttctgaa attccggctg
     2101 ccccggaacg cggcgtacgg cgtttccagc gtaacggtat ggcccaggcg tccaaggaaa
     2161 ccggtcatct cctcatagtt gatgaacccc tccgagctgt aggaaagcan nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccagcag
     2281 ccggaacagg gcctccggag catgctggcg gctattgtac gtggaacgtt tccaatctgc
     2341 gggaatgccg gaaactcggc tggtttcctc cggcttttca taagaagaca gcaaattcag
     2401 catgaaataa ttggaaccgt acggatgctg gttataggga gggtccaggt acaccacatc
     2461 catttccggc agctcggcag ccagttcatt ggcgtcacgg caatggatgg aataccggca
     2521 gtcaaaccgg gaaaacacgg gaaacggcag cgaaatttct cccagaatgc gggaaagggc
     2581 gttgcctccc gttcccccga atttgccgat tccgttcctg tctttgtaaa aacctttgaa
     2641 tacgccggat gtgtttgtgt ggacggaagc ttccgccaga agaggagcga taaaataggg
     2701 cctgacttct gccggaagca aattgatggt ctgcctcgcc gtatccaggt acacggcgtt
     2761 gcggcgcgta tagaaaacgc ggtcatccgc cgtgatgcag tccggatttc tgggagcgta
     2821 cagttccgtg atgaagccag gggattcatg cgt
//
LOCUS       DAWEMX010000211         2803 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_11692_length_2803_cov_9.817686, whole genome shotgun sequence.
ACCESSION   DAWEMX010000211 DAWEMX010000000
VERSION     DAWEMX010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11692_length_2803_cov_9.817686"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          814 a          796 c          585 g          608 t
ORIGIN      
        1 gggcggtccc tactgggggc cgggcttcta ccgcccctat tatccttatt acaatgatgt
       61 cacctatgtg ccggtaaaca ccggatacgt gcttttcatc aacggcaagg tgcaatcctg
      121 ggaacgcagg cgctgacgga aacgaagcct gccctatggc acggaatgcg ccggcgcagc
      181 agaaggaggg caatctcccg ctgatgcgcc gtttttctct ccgcgtttcc aaataccact
      241 ctaatccccg cccctggaca gggggcgttc cagttcctcc cggaatttgg gaaagaactc
      301 cttcaattcc ccggaaacct tcggatattc cagcctcatc tccgccaggg cctccacaat
      361 ggcgcacatg cagacaagcc gggaatacca tttgttgtcc gccggtacca cataccatgg
      421 agcctcccgg gaagaagtat ggcgtatcat ctcctcatac gctttctggt actggtccca
      481 gaattcgcgt tcatgaatat ccccttcaga aaacttccaa tttttgtccg gagtatccag
      541 ccgtgccaaa aggcgttttc tctgttcctc tggggaaagg ttgaggaata ttttcacaat
      601 gctcgtcctg ttggcgagca ggtggcgttc caggttattg atggatctga acctctcctc
      661 ccagaaagca ctcttggcat gggagggatt aaatccctcc ccggccagaa actccggatg
      721 cacgcgcacg ctaaggactt cttcataata ggaacggttg aaaatgccaa tcatcccccg
      781 cctggggagg cgtgctacgc accgccacag gaaatcatgg ctccgttcca gcgtggaagg
      841 ctgcttaaag ctgctgacta cgcacccctg cggattgacg cctcccatca catgcttcac
      901 aatgccatcc tttcccgcgc tgtccatcgc ctgcagcacc accagcagcg catgagactc
      961 gctggcatac aatacttcct gaagctgctc cagcaattcg atgccctttt ccagcttatc
     1021 cacagcctct ttcttgctgt ctttgcccag cttgccgaga tcgccgggat catagcggga
     1081 caacttgaat cccctgccgt ccgtcacccg gtacggttca ataaatttcc tgcatctttt
     1141 gaccaggcgg gaagaaatat ccatcatttc tataatgaca ctgtctcttc tcaaaatgtt
     1201 gaaacaacag caaaaacatc aatacccctc tcgttgggag attccacccc gccgataaac
     1261 gacaaacccc cggcataatt cggaacgttc atacttgccg gaagactcta tggactggag
     1321 aaaagcagca taatcggatg gccgggaaac tacgatcacc ttataatcag accatttgtc
     1381 ctttaacggg ccgggaatcc atccataaaa ctcctttccg gctccattgc ataaaagtaa
     1441 aatgtcaaca tgactgctct gcaataattt gcaataagat accgcagagc ggaaatcttc
     1501 acggccatac aaatcattgc aacgaatacg aatatcgctc accgcccaaa caatcatcat
     1561 taataggaac aggatagttt gggcaatcct ggggaacgac caatcccatg acgccaccag
     1621 ggaccaggcc agacacaata caggcaataa cggcgcgcaa tgccgtccgg aaaaacggaa
     1681 atccatcatt tccgttccat aaaggaatac ggcccctgga agcaatatca acaccagcaa
     1741 agcaggaacc aagggcctct cagcccgctt attccacaaa atgatgccgc aggcagaacc
     1801 ggccagaaga atggcgccgg agatcatccc tgcccccaat ccgttcatat tccataaagc
     1861 atcaggagtc atgcacatcc tcaattctag acgggaaggc cccatgccgg ccaatcccga
     1921 taactcgtac atggaagcgc atgcattgac tatccaggag gaagatatgg aaaccgcacg
     1981 ggcgcccaag aaaagagtgt aaagataata tgctcccaac ccggaaaatg gaaaaatcca
     2041 ccagagcagg gctcgccgaa acctccgccc tccaaactgc cggaatgcca tgaccatgaa
     2101 tgccgcggca aaaccgagcg cccatacaac ccctgtcagg cttgtaagac ataaaaaaaa
     2161 cagagaacca aaaaagacat ggaattttac cggttctccc cggctaacct gccaaaacaa
     2221 catggagaca gcgcaagagg ccgctatctg caacatatag ggccgcaact cgttagaata
     2281 ataaacaaaa aacggtgaaa tcaaaagaat caccagcgcc tttggttcct ttctgaaaaa
     2341 ccaggatgat aaaaataccc agaatatatt aaataacctc agcatccatt cagactccgc
     2401 acccgtcaga tgatgccaca aaaataaaag gtagtaataa aaggccattt gagcatcgct
     2461 accgccaatt tcacaaatct tcttccaaca tccctccatt ccctgctgca tggcgcacaa
     2521 tgcagtacaa cattcatcaa tccaaaaact ttggttattc agggaaaaaa agaaagacaa
     2581 aaaaggtaaa atccaccaat atttgatgcc cccgtcattg ccttccgccg ccctcttttg
     2641 tcccctgcac atagtgcaag gcaacatagc ggattgcata tccggtggca acatattttt
     2701 ctaagataaa aacattaata acttttcaaa gttgcagaga taagaagaaa aaacaaatct
     2761 attttcatat ttatctattg atattcatat ggatatgtga ttt
//
LOCUS       DAWEMX010000212         2755 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_11874_length_2755_cov_9.633333, whole genome shotgun sequence.
ACCESSION   DAWEMX010000212 DAWEMX010000000
VERSION     DAWEMX010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11874_length_2755_cov_9.633333"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          603 a          886 c          718 g          548 t
ORIGIN      
        1 ctccggaacg aacatgtccg ccggccttcc ccaacacctc acgctggatt atctgcgcgc
       61 cctgctatcc cggggcgtgg agaaaaccac cgtcacggag gaagccagaa tggtcctgag
      121 aaaatgggtc atggacgcgc gccggatatc cggaagccct ctcccctctt ccgtttccgc
      181 caagcctgcc gcaaatgaaa cacggccgca acagcccacc ccggagcaga tgcctccaga
      241 ttccgtggat gaagcatcct tcggcaatga actccgggat atcctcaacg gagtacagcc
      301 gcacaaaacg gaggaggaag ctccggtgcc ccgccatatt tcctttgatc tggaaggtga
      361 aacggaggaa gaaaaactgt cctccctgcg ggagctggtc gttaactggc cgccgctccg
      421 gaacatggat tccttgaggg aaacgcccgt gttttcctcc ggcaatccca gggcggacat
      481 catgatggta acggacgccc ccggcctgta tgaagaaaaa cagggggttc ccctggccgg
      541 gccttccgga caaaagctgg acgccatgct gaaagccatg gggctttccc gttccgatat
      601 ttatctgacc catctggtca aataccgccc ggccctcccc cggcagctta ccaataaccg
      661 cccgcctaca gaccgggaga tagaaatttc cctgcccatt ctccgggagg aaattatgct
      721 ggtgcgcccg aaagtagtgg tggccctggg agcaatctcc gcccgcggca tcctccagtc
      781 aggagagacg cctctttccg ccctgagagg caccttccac acagctttca acacgcccgt
      841 gcgcgttact tataatccca gttatcttct ccgcacggaa gatatttcag aaaagcgaaa
      901 ggtttgggag gatatgctgt gtgtcatgga acaggcaggc ctgcccatct ccgaaaaaca
      961 acgttcctat ttcctgccca aaaagtaata tatggattat ttaaacagta ttctggagcg
     1021 caaacgcgcc gatttgaaaa aacttcttcc cctggaagga aaattacggg cctccgccat
     1081 ccagcggaac gactacgcgg gattccgcac cgcggtggac cttggggaaa cccgcctctc
     1141 cgtaattccg gagatatcca gggtacatcc cgccctccgc ctccgggaag cagcccagga
     1201 tatccggcac atgtacggca tgttcctcca gggcggagcg cagggcattt ccatcgctgt
     1261 ggaaccggaa gtatacggcg ggtcatggga catggtttcc actgtttcca gaatcagttc
     1321 cattcccgtc atggcgcgcg acgtgttcat tcatcccgcc atgatctgcc aggccatcgt
     1381 ctcgggggcg gatgccgtca acctggtggc ggccgccctg ccggcaaagg atttgtcgga
     1441 gctgtaccgc atggcttccg ggctgggatt ggacgtgatg atggaggtcc ataccctgaa
     1501 ggagcttgaa accgtcatgg acctggaagc ggaattcatc tgcatcaaca atgcagaccc
     1561 gcacactctc caggccaatc ctgcagtcac ggagaaactc attgaagaaa tacccgcctc
     1621 cgtcactgtc ctgtccgcag ggggcatctg caccgtggaa gacgcgcgcc gcatgctgga
     1681 cgcaggagcc aatggcgtcc ttctccaaga cgaacttgcc cgcatggata ttccccagga
     1741 tttcatggag gccattctgg ctctccgggc ggactgagtt tccactcccg ctatattata
     1801 atagaaaaac atctgcatcc gcatggggaa atgcggggct aaagagattg tcccgtccgc
     1861 tgcggcaggg cttcgcgacc tgagctccgt atccgccgcg tttccttccc gcctttccct
     1921 gccgtctttt catgaagcgg gcaaatcctt cgccccgaat gctcctcgga tatgccagaa
     1981 ttccggaggc atcccttccc tgtacaggac ttccacaaca tcgtaacggg aattcaccgg
     2041 ttccggaagc aggcgaagcc attccgccgc tccccggcgt atcagcgccc ttttacgggc
     2101 attcacagcg cggcgggcac ctccataacc attccccgta cgggttttca cctccacaaa
     2161 aaccaggcac tccccttccc ggcacacaat gtccagctct ccgcctctga cgggacgcca
     2221 gtttctcctc aggataacgc acccttccgc acgcaggaag gaggccgcag ccagttcccc
     2281 gtacattccc agccatgccg tatcccgctt acccctgctc agggatggac agttccattt
     2341 gtgaaaccgg cgcaaacgag cggcgatgga gcggacaggg cccatgccgc cgcagagctt
     2401 ccatgtgggc ttttgtgcca tatcctttgt gttttgcaaa accatactgc gggtaggaag
     2461 cgtccgcttc catcatcatg cggtccctcg tcaccttggc cagaacgctg gcggcggcta
     2521 tggaaaggct cctgccgtcc ccctttacaa tggcgcggtg cggcacagga agccccttga
     2581 caggcaatcc atccaccagg cagaaatctg cacgcagggc cagaccttcc accgcacgcg
     2641 ccatcgccag atgagtggcc ctcaaaatgt tgaaccggtc tatttcttcc acggaagcca
     2701 ctcctacgga acacaccgcc tgttcacact ccaaaagagc cagaaacagg cgttc
//
LOCUS       DAWEMX010000213         2729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_12004_length_2729_cov_7.992147, whole genome shotgun sequence.
ACCESSION   DAWEMX010000213 DAWEMX010000000
VERSION     DAWEMX010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12004_length_2729_cov_7.992147"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          608 a          699 c          819 g          603 t
ORIGIN      
        1 ctcctctcat tgacggtgcc tttcatcctt tgctgtaaaa cgtccggttt tcttcaggag
       61 aagccgggcg tttttctgtt ccggtcttgc ggtatctctt ttccttatta cgtgccagtg
      121 gcgggaatgt attgtaaaag cgggcagtat aagtgaaacc ccgtgtttca tccgtattcg
      181 ggatgatact ggccttctta gaccattggt tatgaataaa agttcaaaga atgtcgctga
      241 aaatatatga caggaacatt ttgaatgtat tcattgcggt ctgggtcata tctgcatgcc
      301 tgaatttaca aatgcattta gcggattaaa ggaaaaccgg aaactcaccc atgacgaatt
      361 ggtgagagct atccggttca tgattgcagc ggaatatgaa gctatccagc tttataccca
      421 gcttgcagaa tccatagacc ataagctggc ccaggaagtt ctgctggata tttccaatga
      481 ggaaaaggaa cacgcgggcg aatttctccg cctcctccag gtgctggccc cggacgagca
      541 gggcttttat gacgagggga tgggggaaac gaatgaaaag atagacaagc tgaaggcgtc
      601 ctgcccggac ggcgcatgca gcagttccgc cgcccattga cggcaccttg ctcatggtta
      661 aagtgcccgg ttcccctgca aagggggccg ggtattttca tgacgccggg cttttgcttc
      721 cagctcggct gcgcagcggg cgcagaaggc aatgcagcgt tcacagggtc gggaggcgta
      781 ataggcggca gtcctttctc cggggcgcgc cgtttccgct gccaggctct ccggagacag
      841 ccccagcagt tcccggcagt aaagagtgcc gaattcctgt ttgaaggagg cggccaggga
      901 ttggaccagg gaaaagattt tttccttttc tccagtttcc ggggaactgt ttccgtacag
      961 gctccccgcc agcatgctca ttccgcaaaa agcgccgcat acctcccgca tgcgtccaat
     1021 gcctgccccc aggggagagg acagtttcag ggccgtttct tccgtcaggc cgcagacatc
     1081 tgcaaaagca gcaaaaacgg attgggaaca attatatccc tggtggaaat actccagcgc
     1141 ccgggctgcc cggtcttcct tggaagggat catgggaaaa ggggggatta ctgctctttt
     1201 tctgcggacg tgtccgctgc ggctttttcc ggcttgttgc ggtccgtcag acgaagcatg
     1261 agtatgtaac agacggccag aaccagcacg gccagaataa gggggagact ttcgctcttg
     1321 acggtgcgga tcagctcctc cggagatttg agcaattccg gatgccgttc cccgatgcgg
     1381 tgcccgtacc aggcgagcac cgtacaccag gcaaacgagc cgagcacggt gaccagggag
     1441 aaaatgccga atcccatgcg gacaatgccg gcgggaatgg aaatgaggtg gcggatgacg
     1501 ggcagaaggc gggcgaagaa gacaccggaa acttcgtacc gctgaaggaa gatttccgct
     1561 ttttccacct tgtggggcgg catgaaaaag aatttgcccc agcgcatgat gaccgggcgg
     1621 cccacgattc gggccacggc ataggtaatg gcagaaccca gccaggagcc gaacgttccg
     1681 gcagccacca ctccccagaa gctcatggat gctccttcct gggcggacag gatggccgcg
     1741 ggcgggatga ccacttcgct gggaacggga aagatggagc tttccatggc catcaggacg
     1801 atgacacccg catatcccca gtcctgcacc caggacatcc aaagtgagat caactcatgc
     1861 ataacggcgc gtatcttgca ttaaaatcct tgaaaggaaa gtcttttatt gcgtcccggg
     1921 aacggggctg gtaaggtagg gaggcgtccg gcgtgcgccg atgcagatag tgatgaagct
     1981 ttatattata gcaggcgaaa agagcgggga cattcatggc gccctgctgt tgaagaatct
     2041 gctgcgcctg atgcccggca tggaggtcgc agggctgggc ggccagggca tgcacgcgtt
     2101 atgtcccggc gtggaagact gggcggatga agccgctgtt atcggcgtgg tggaggtgct
     2161 caaaaaatac ggatggttca gaaggcgttt tctctccatt ctggaaagga tacggcagga
     2221 ccagccggac tgccttgttt tgattgatta tccggggttt aatctgcggc tggcggagaa
     2281 ggtccgcaag tgctgccccc gcaccaaaat cgtttatttt atctccccgc aggtgtgggc
     2341 atggcatcgc gggcgcattc ccaagatggt gcgcatgctg gatttgatga tgtgcatctt
     2401 cccttttgag gctcccttgt tccaggaagc cggactgaga acggagtttg tggggcatcc
     2461 cctggtagat gaagttgctt ccattcgtaa ggagggcgtc cgggatccgt cactggtcgg
     2521 tctttttccc ggcagccgga accgtgaaat tgacaggcat ttcccggtat ttatagaggt
     2581 agtgaaccgc ctttcccggg aacggccgga actgtctttt gaaaccgccg cttccacgga
     2641 ggctctggcg gaaagaatgc gcggcatggt gcggaaggcc ggcatgcctc cggaactgtt
     2701 ccacattgcc gtgggaaaat atcatgagc
//
LOCUS       DAWEMX010000214         2632 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_12453_length_2632_cov_8.522313, whole genome shotgun sequence.
ACCESSION   DAWEMX010000214 DAWEMX010000000
VERSION     DAWEMX010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12453_length_2632_cov_8.522313"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          512 a          884 c          704 g          532 t
ORIGIN      
        1 cttccggtat atccatgtct gcggattccg ggacggcggc gtccgcaggg tcgtaaacgg
       61 aagggttgcc ttccagcacc tccctgaggt atttgcccgt gtatcccttc ccggcggcta
      121 cgatttcctc cggcgtaccc gtggccacca catggccgcc tccggcgccg ccctccggcc
      181 ccaggtcaat cacgtagtcc gcggatttga tgaactccgg gttgtgttcg atgaccagca
      241 gggtatgccc ctgctccacc agttcccgaa acacggccag cagaacttca atgtctgcaa
      301 agtgaagccc cgttcccggc tcgtccagaa tcagcatctt ggagctgttc gcgccggaac
      361 ctatctggtc cagcagaatt ttagccagct tcaggcgctg gttttccccg ccggaaaggg
      421 tattcagagg ctgccccagg gtcaggtgcc ccagccctac gcgctgaagc acgcccagtt
      481 tggaagcgat gcgggaggct ttggcccctt tttccgcgct gaagcattcc agcgcagccg
      541 ctaccgtcat gccgagcacg tccgcaatat tcctgccgtc acggtacacg ctcagcactt
      601 cgctcccgta ccgggagccg tggcacagcg gacactgaac gaaaatgtcg gaaagaaact
      661 gcatttccac tttctcactc cccatgccca tgcagcgcgg acaccggccg tccccactgt
      721 tgaaagagaa aaaaccgggc ttcatgccgc gcgcgcgggc cgtctccgtt tccgcgaaga
      781 gggagcgtat ttcttcaaaa actccggcat acacggcagg ggtggagcgc ggggtgcgga
      841 cgatggggct ctggtccacc atcaccacct catccagatg ttcccagccc ttgatagact
      901 tgacgcgggc gggttcctcc tcgcatacgg ctcctttctc caccagcgcg ttcaggtaaa
      961 gaacatcgtg ggccagggtg cttttcccgg acccgctgac gcctgtcaag caagtgaaaa
     1021 cgcccagggg cactttcacg tccagtttgc gcaggttatg gcgcgtggct ccggatacgg
     1081 tcagaaactg gcgcggcttt cgcctctttt tgggcaccgc gatgcgtcgc ctgccggaaa
     1141 gaaaagctcc cgtcagggat tcctcaattt ctccaatccg ggcaggcatc ccggaataaa
     1201 caagccttcc cccttcccgt ccggaaccgg gtcccatatc caccaggcag tccgcggcac
     1261 gcatgaccgc ttcctcatgc tccaccacta ccagggtgtt cccgcgcttt ttaagccggt
     1321 tcatggcgga aatcaaacgg gacgtatcgc ggggatggag cccaacggta ggttcatcca
     1381 gcacaaacag cgtatccgtc agggaagcgc ccaggcaggt cgtcaggctc acgcgttcaa
     1441 tttccccgcc ggacagggat cgcgtggagc ggtcggacgt caggtagccc aatcccactt
     1501 cattcagata ctccaggcgg ctcctgagtt ccgccacggc atgtttcaag ccggggtctt
     1561 catccgcccg cggcgtcacg tacctatcca cccaggccag cagttcatcc atgggcatac
     1621 tggaaagttc cggcatgctt ttgccgccca ccttgaattg aagggcttcc ggcctcaggc
     1681 gcaaaccgtg gcaggagggg cattcctgat atgtgcggaa acggctcagg tacacccgca
     1741 cgtgcatctt atgcgtgcgg ctttccagat atttgaaaaa tcccgcaatg ccgtaccaca
     1801 gcccctgttc atacatttca tccgggtcat cgccgtcgcc gtacagcagc cagtcccgct
     1861 cccattgctt caaatccttc cacggcacat ccagacggac tttgcgggcg tttttgcgcc
     1921 agccgcgcat caggtcccgc ttgcattcgg aaaatacttt tccgtccccc tcaaaaatat
     1981 ggatggcccc gtcctgaacg ctgagttccg gcttgatgca ccgattgtag tcaatcgtga
     2041 tgacgcgccc gtagccgcgg cattccgggc acgcccccag cggggaatta aaagaaaaca
     2101 ggcccggccg cggttccatc agcggatgcc agtctccgcg gaatttcaaa gcggacaacc
     2161 agacgccgtc cacacgggga atcacatggg ccacgccgcc gcccaggcgc atggccgttt
     2221 ccagagcctc cagcctgcgt tccctctggt cttccgacag cctcacgcgg tcctgaacca
     2281 ccagcagcgg ttctccttcc tccagcgtcc aatcctcatc ctccagccgg aggatctctc
     2341 catgcgcaaa agcgcgcaaa tacccctgtg ccaccagatt ccttttcaac gtgggaagat
     2401 ccacggattc gggccgcgcc acctccaggc aaaccatcac ttccgttccg gcagggaatt
     2461 ccgcatgcag cttttcatca atggtacctg gcgtttccgg cttgatttcc cgccctgttt
     2521 ccgggtcaat gcccacggcc agacgggcga aaacaaactt ccagtaatca ttcagccccg
     2581 tcatggtccc caccgtggag cgggatgtcc tgacggagtt gcgcggatgc ag
//
LOCUS       DAWEMX010000215         2581 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_12735_length_2581_cov_8.054632, whole genome shotgun sequence.
ACCESSION   DAWEMX010000215 DAWEMX010000000
VERSION     DAWEMX010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12735_length_2581_cov_8.054632"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          503 a          709 c          830 g          539 t
ORIGIN      
        1 ggtatcggga aggacggtct ccccctgcgg agaatgcgtg tccatctggc tgatgagcac
       61 gttcaggtgg gcggggcggc gggaggcggg gatgtgcagc gcggcgccgt ccttcgtctg
      121 ccgggcaaaa ctcagtccca ccgcgtctcc ctgccggatg gcaatgtaag ccaggttggc
      181 cgccagcctg cacgcgcgca tatacttggt ctccacctgc tccggatgat aattcatgga
      241 gccggaaaga tccatgacga tgtaggcgcg caggttggta tccgcttcaa actccttgat
      301 gtaatacctg tccgaccgtg catacgcttt ccagtccaga cggcgtgtat cgtctccggc
      361 cacgtatttg cggtattccg caaactccac ggaggagccg cggctggcgc ttctatggcg
      421 gccggatacg ttccccgtca tgctgtggcg cgcttccaag ggaaggttcc ccaggcggct
      481 gagcaggttg tgattcaggt atttcatggg gcagggaaag gggattgatg atgccgggac
      541 ggcggtcaga tttccgccgc caggcgcgcc acggccttac ggggcgtgat gccctgggat
      601 tccgcggcga agttggtgac cacgcggtgc atcagcacag gttccacaat ggcgtctatg
      661 tcttccctgc ggacgatgta gctgccctgg agagcggcgc gggtcttggc cccaagaatc
      721 aggaactgga cggcgcgggg gcctgcgccc caggctacat actgctttac ccattccggg
      781 gaagtttcca gggtggggcg ggtggcgcgc accagtttca cggcatactc gtaaatatgt
      841 tccggaacgg gaacgcggcg caccagatcc tggtaaaaga tgatttcctc cccggagagg
      901 atgtgcttga gttcctcgct ccgggttccg gtggtttccc gggcgatgcg catttcctcc
      961 tcctccgtgg ggtagtccac ttcaatcagg aacatgaagc ggtccagctg ggcttccggc
     1021 agggggtagg tgccttcctg ttcaatgggg ttttgggtgg caagcacgaa aaaggggggg
     1081 agcagggtga aggtgcggcc catgacggtg acgcggcgct cctgcatggc ctccagcagg
     1141 gcggactggg ttttggcggg ggcgcggttg atttcgtccg ccagcacgat gttggcgaac
     1201 acgggccccc tgatgaactc gaactggcgc ttgccgccgg cggaatcatc ctggatgatg
     1261 tccgtgccgg taatgtccgc gggcatcagg tccggcgtga actggatgcg gttgaaacta
     1321 aggtccagcg tttttgccac ggaactgacc agcagcgtct tcgccaggcc cggaactccc
     1381 atcaggaggg catgcccgcg ggaaaacagg gcgatgctca gcagctccac gacgcgctgc
     1441 tggccaatga tcattttgct caattccgag gaaagctcct tgtaaatggc gccgagccgg
     1501 tcaatggcgg ctacgtcgtt gtcgggcaga gtgggggtga cgggttccat attgcaattt
     1561 catagcactt tccgggccgt tttccaccaa ataatcgcgc cgcgttcatt ttccctgctt
     1621 ggaatgggaa gggaaacggg gtaaggaagg ggcatgccga aaattgcact cattcaactg
     1681 tccgcagatg cccggccttc cgtgaacaag gaaaagacgg aatccgccat ccgtgaagcc
     1741 gccgccaacg gcgcgcagat tgtctgcacc caggaacttt ttaccacgga atacttttgc
     1801 aggacggagg aatgcgattt attcaatctg gcggaacctg ttcccgggga attgacggaa
     1861 gcccacagga aactggcggc ggaactgggc gtggtgattg ttgcctccgg ctttgaaaag
     1921 cgcgccaccg ggctttacca caataccgcc tgggtggtgg atgcggacgg ttcgttcctg
     1981 ggcatgtacc gcaagatgca tattccgcag gatcccgggt ttgaagaaaa attctacttc
     2041 acccccggcg acctgggcta taaggcgttc gacaccagat tcggccgcat cggcgtgctc
     2101 atctgctggg accagtggta cccggaagcc gcgcgcctga cggccatgca gggggcggaa
     2161 atcatttttt accccacggc catcggctgg ctaccggagg aaaagcccct gctgggagaa
     2221 cagcagcact gcgcgtggga aaccgtgcag cgcggccatg ccgtggccaa cggatgctat
     2281 gtctgcgccg tcaaccgggt ggggacggaa ggggaaagcg aattctgggg gcagtccttt
     2341 gtggcggact actatggcca gattatggcg aaggctcccg tcagcgatga agctatcctg
     2401 tacgcggacc tggacctgga cgcattggaa gaccaccgcc gcatctggcc ctttttccgt
     2461 gaccgccgca ttgactccta cggcggcatc acggaacggt ggggaaagta atgtatccta
     2521 tatattttta tacaaaaatt tggtatttgg aaaatacttg ttgacatttt ttattatctg
     2581 a
//
LOCUS       DAWEMX010000216         2491 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13247_length_2491_cov_9.272167, whole genome shotgun sequence.
ACCESSION   DAWEMX010000216 DAWEMX010000000
VERSION     DAWEMX010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13247_length_2491_cov_9.272167"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          562 a          750 c          682 g          497 t
ORIGIN      
        1 tggcagaaag ccccgtttcc gcgaaaagct tttctttcgt ggtccgcatt attcaatgat
       61 gcaggcactt gaaaaagcag gacgtcgagc gggaattgct cagccagctc ctacgttccg
      121 gtgcgggcat tgccgccaat ttatccgaag ctgcttacgg gagccgcaga agggacttct
      181 tttccaaacc ccgcatcgcc ctgaaggaat attccgggac gcatgcacgg cctaaactgc
      241 tgcgcgccac gggaagcctg acggaaacgg aatcttcctc tgtcaaccag gacagcacag
      301 aaatccctaa aatgctcatt tctattaaca aaccaactca acaagctcag ccggattcct
      361 attaactttt cactattaaa tcttaactca tgtactccct ctgcttggac tgcggcgcta
      421 ctaatgtgcg cgccatggtt gtggataaac aaggcgtcat tgtggggaag gcctcacagc
      481 cgaacgccac gctgcccggt gaggaaaatc cggaatttca cgtctgggat gccgacctga
      541 ttttcagaca gctctccgaa tgtgccgtga aggccctgga agggctaaat gcggaacagg
      601 tgacggccgt cacgattacc accttcggtg tggacggagc actggtggat gacgccggca
      661 acctgctcta tcctgtcata tcctggaaat gcccccgcac ggcggaggtc atgaaaaaca
      721 tcggcaaata tatctcccaa gaggaactta accgtatctc cggcgtgggg gcctttgctt
      781 tcaacaccat ttacaaactc gtctggctca gggaaaacca cccggaactt ctggaaaaag
      841 cccatgcgtg gctcttcatc tccaacctgc tggcctacaa gctcaccggc atcatggcga
      901 cagaccggac catggcaggg acctcccagc tcacggatct ggaaacggga gatttttccg
      961 aattcatttt gaactgcctc ggtttgagcc gcggcctctt ccctcccatc gtggatgcag
     1021 gggaaaccat tggcctcctt acgccggaag cggcagacgc gatgaatctc ccggccctgg
     1081 cgggcattcc cgttatctcc gccgggcacg acacccagtt cgccattttc ggttccggag
     1141 cagacaagaa ccagcccgtc ctttcctccg gaacatggga aatcctgatg gcgcgctccg
     1201 cacaggccag gctggcccgg gaagattatg aggacggcgc cacggcggaa ttcgacgcgg
     1261 aatccggcct tcttaacccc gggctccaat ggctgggctc cggcattatc gaatgggtga
     1321 aagcagcctg ctttcacggg gaatcctatg acaccatgga tgcggaagcg gccgccgtcc
     1381 cacccggctg cgacggcgtg acaatggttc cggacttcct ggcctccgga gaccggaaag
     1441 gctccatcaa cggactggtg ctgggaagaa cacgcggcca tatctaccgc gccgccatgg
     1501 aggctctcac ctggcggctg aaatcccgcc tgcgccgttt ggaatccgtg ggcggcttca
     1561 aaagcgaatt cctcatcctg gtgggcggag gcgccagaaa cagagtttgg acccagatga
     1621 gggcggacat tctccggatt cccgtcaaag tctctgaagt gtcagaaagt acggtattgg
     1681 gagcatccat gtttgccttt gcgggcgccg gggtgtactc tacgccggag caggcccggg
     1741 ccgccttcgg catcacctac cggacatata tgccgggttc ccaggaagcc gcctacgaaa
     1801 agttcaacca ataacacgct actaaatatg tcagaagact ggtcaatctc ccctcccctg
     1861 ccggacaaag tgttcccatg ggaaaaatac aaccccgtca cgcgggagga agtgaatgaa
     1921 ttcttccatt ccccggaaat tctggtcatc aaggaacgca tgtgcgacat agggcgccgc
     1981 ctgtggaacc gggaatatgt ggacggcaac gggggcaaca tctccgtgcg cgtggctcag
     2041 aacctgctgc tctgctcccc caccctgtgt tccaaaggtt tcatgactgt ggaagacatc
     2101 tgcatggtgg acatggacgc gcgccagaaa gcgggcatcc gcccctccac cagtgaggtg
     2161 aaaacgcaca ttgccatgat gaaatccgtg ggcgtcaatg cctgcatcca tgcccatccc
     2221 ccgcactgca atgccttcct gtttgccggg caggttcccc cctccggcat caatccggaa
     2281 gcggatatct tcctgggaca catcccgctg gccccttacg gaacacccgg ctctctggaa
     2341 acggcccggg ccgtggcgga agcagccaga caatccaccg tcgttttcat ggaaaaccac
     2401 ggagtcatta ccggagcacg tgacgtggaa gaagcggaat ggttcatgga aaatgcggat
     2461 gcctactgcc gcatggtgct gatggccgga c
//
LOCUS       DAWEMX010000217         2485 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13280_length_2485_cov_9.130453, whole genome shotgun sequence.
ACCESSION   DAWEMX010000217 DAWEMX010000000
VERSION     DAWEMX010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13280_length_2485_cov_9.130453"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          570 a          675 c          711 g          529 t
ORIGIN      
        1 tctgccggga acggttttga cgggcctgcg cgataagata ttttgctgaa atggcaaagt
       61 tctccatgag cttgcttgcg acaaggttcc gcgaacggcg gaatagtttc gtaaatcaat
      121 gatttatgct ttgacaagag agggggcggg aagtatattg cccgtccctc gcgtgattcg
      181 gcgcaactaa acaacgggga tcggaggggt aaaaagcgtc gaagtcccca aaaactgctt
      241 ttcctgccgc aaggaaagcc acctacatac atatatgatt aatgatctga ttaaccagat
      301 ggttgaagcc ggtgttcatt tgggccacca aacccgcaaa tggcacccca gcatgaaaaa
      361 gtaccttctc aaggacaagg ccggtatcca tatcatcaac cttgaggaaa cggaaaaatg
      421 cctggacaag gcctgctcct tcctggctga tctggcccgc cgcaataaga aaatcctctt
      481 cgtcggctgc aagcgccagg ctcaggaagc cgtccgcgaa gctgcggaag ccactggtca
      541 gtactatgtc aaccaccgct ggctgggcgg catgctcacc aacatgagca caatccgcaa
      601 gagcattgaa cgccttgtct atctggaagg gattgaaaag agcccggaat tcaagagcat
      661 gtccaagaag gaactggccg ctctggaccg cgaacgccag aagctggaac gcaaccttca
      721 gggcatccgc aacatgggcg gcgctccgga cgccatggtg gctatcggcg ccgaccatga
      781 agacatcgcc atccgcgagg cccaccgcct gaacattccc gtctgcgttc tggttgatac
      841 caacgccgac ccgaaggaaa tcgacttccc gattcccggc aacgacgacg ccgtccgttc
      901 catccgcctg attcttgact gcattgtcaa ggccatcaac gaaggcaagg gcgcatccgc
      961 cgaagcctaa accaaccatt tagaaagaac agaaacacta tggcagaaat tactgcagct
     1021 ctcgtcaagg ctctccgtga caaaacggac gccggcatga tggattgcaa gaaagccctc
     1081 aacgaatgca acggcgacat ggatgccgcc gtaaagtatc ttcgtgagaa gggaattgcc
     1141 aaggctgcgg ccaaggccga ccgtgacgcc aaggaaggcg tgatccgcgc cgctgtggct
     1201 ccctgcggct gctccggcat catccttgaa ctcaactgtg aaacggactt ctgcgccaag
     1261 ggggacaagt tccagggtct ggtgaatgac gttgctaacg ctctcatgga ttccaaggcc
     1321 tccacgctgg aagaggctct ccaggtcccc atggcggaag gctccacgga agaatatatc
     1381 aaggccatgt gcagctccat tggcgaaaac atggctctgc gcaaattcgc ccgcctgacg
     1441 gcggacggca tcggcgccgt ggtctcctac atccacatgg gcggcaaagt gggcgttctg
     1501 ctgtccatca aggccggcaa ggaagaaacc gtcaagtctg atgcgttcaa agccctcgcc
     1561 aaggatttga ccttgcacat cgccgccgcc gctcccaaga gcgtcagctc cgatagcctg
     1621 gatcccgcct tcgtggccga ggaacaggaa atcgccaagc agcagctcat tcaggaaggc
     1681 aagcccgaac acctgctgga aaagattctt cccggcaagc tgaaggctct gtacgcccag
     1741 tcctgcctgc tgaaccaggt atttgtgaag gatcctgccg gaaagatgac ggtggaggcc
     1801 ctcctcaacc aggccggcaa ggaactcggc gacgatatct ccgtcgcctc cttcgtccgc
     1861 taccagctcg gtgcgtaagc tctgagtcag agtatctctg gaaccgttcc ctttgcgggg
     1921 aacggttctt ttgtttgata tggctccatg gggggattta tccatgaatt tgattcaggg
     1981 aggatccgga aatccggata ggggatcatc aaaatatgcg ggatagaaaa gctccggccc
     2041 tgctgctggt tgcggccttc atcctggcag ccgtttttgg agcgcttttg ggctggagca
     2101 tgttttttcc ttgtgtgcgg tccctgatat ttcctggcgg tattatggtt cttcaaaaag
     2161 gccaatggat ggtggaaatg ggggaacagc ctcttttgct gttatcagcc tcgtgtgtga
     2221 tgatgaactc ttctggttcc gggtggctcc ggaagacacg cgaaatgccc agccggggct
     2281 ggcgttcatt gccggtcctg gtttttctgt gggggaaatg gcatgggagg tgcgcatgga
     2341 tgtcctgata ttggaggaac tggcgcggct ggggtcttgt cttgcatcgg aaggcttgtt
     2401 agggtggcgg catggctttt ctggaactgg atcatgtgag cgttgtcttt cctgcggtgc
     2461 aggggttgct ctctgcccgc aaatc
//
LOCUS       DAWEMX010000218         2473 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13353_length_2473_cov_11.766749, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000218 DAWEMX010000000
VERSION     DAWEMX010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13353_length_2473_cov_11.766749"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          505 a          608 c          777 g          583 t
ORIGIN      
        1 actcttcaag atattgttcc tgattgtaaa cgggaacgat gacggaaacc tggggctgtg
       61 gcgggcgggt tcgggtcatg ggcgggcctg ttgaggtttg gagggggagc atgcggcaag
      121 ggcccgctcc agattggaac gggattccct gtcaatatcc ctgcccagtt ggagaagcgc
      181 ctgctgcatt tctgcgtatt cctcttccgt ctgcctgatg gcgcggagca tggcgccgcc
      241 aagcgtttct tccctgtaaa ggagtgcatt cctgtcgttg aagccgtaga aggaagcgaa
      301 tttctcatgg atgaccggga ttttcgcgaa tccgtaaaca agctgggcgg aaccggtgac
      361 gccggtggta atgtagcggt tatgcgccgg attgccggga tcaagcaggg gcaggaaata
      421 gtccgcccgc ctcatggcct cgtacatggc ggggaagtcg agctttccgg ccacatgcag
      481 gaaggggcgc agatggccgg ggatttttcc cagctctcct tcccccacga tgatgacgga
      541 aaagttcctg tgcccttcat tgcagacggt ttccagcgct tcaaccagga gttcgtggtt
      601 tttccgctgt gcggagaggg atcctacggt gatgaacatg ggttcgcggt tccggggcgc
      661 aggcgggatt tccccgaaaa agtgcggatt aaccatcatg cccctgggaa aggagcccag
      721 cgtcagcagc cgtccctggc ggtcaagttc ttcttccccg aaccggggga tgtcctttaa
      781 ttcatgttcg acggccagaa gattggcgaa tttgtcaagc cccgtcatgt gaaggaagct
      841 tgcccaaccc ggaatgtcat aaaaggcgga tgttgatacc aggatggctt cgtaccgggt
      901 gaggacgcgc gctttggcca ggcgcttcca tgaagtgcac atggccgtga aaatatggat
      961 atcgccttcc ttgaaacaac acagggggga ttgcctggcc aggctgtgat gcatcaggac
     1021 atcaacgcgg tatcccatgt cgagcaggtg gcggcagaaa ccggggatga cttccccgtg
     1081 gcaatagttg ggttccagaa taaggatggt gcggccggcc actggatgcc tgcgccatgc
     1141 gcgcatgctt cggcgggcgt cccggtcata gtcggacagg aaatcgcgca ggaaagtgcg
     1201 ccatttttta cgcaggttcc gaaacggaat acacatggtc agcaatcgga gcatgactgt
     1261 ctgcgtatgt gtcattggtt tcatggtgaa aaattgcagg aaatataatg aatatcgcct
     1321 gacgcggatg atgaatggcc cttgttaccg ttccgggcgg tttttccccc gccgtgtaaa
     1381 aaagaatacg ttgtgtgacg agggctgaat acttaataaa ttcatactga aaagcaatat
     1441 caaaaaaact ccttggcgca tgatgccgga gtattcctga cggtttgaat ttgccgccgt
     1501 attgcagccg taccacagcc ctccgtttcc gttattcccg ggaacatcct tcgggaggca
     1561 gcctttgcat ttctttccgg aaacagtttc cctcgtccgg ttgttccgtt tttcaacaga
     1621 tggcgttgct gttttttctt gatgtgagtt ttcaatccgg ataaaaaagg atgcttccgc
     1681 catgagcgta tccgtttcca tcattgttcc ctgttataat gttgccgctt atttggacca
     1741 gtgtatggaa agcctggccg gccagagcat ggaagatatc gaaatcattt gcgtcaatga
     1801 cggttcttcc gaccatacgg cggagatatt gcgcgaatgg cgggacaggg acggccgcgt
     1861 ccgggtaatt gaccagaaaa acagcggtgt ttccgcggcg aggaacagcg gcatggaagc
     1921 ggctgcggga aagtatatcg gctttgtgga cccggacgac gtagtggagc ggaatatgtt
     1981 ccggcgcctg tttgatgcgg ccgtggaaaa agatgcggac gtagcggtgt gcggatacca
     2041 tgagttctgc gaccgcggag gcatggatat gcctgaaagc ggctggtcgc cgtcagccgg
     2101 gtttttctcg gaagaaaaag cggagcggtt ccggcggggg acgccctgga gccggtgcgc
     2161 gggaacggtg tggaataagc tgatccgcag gaagctgttg gaagaaaacg gtgtgcgttt
     2221 cgttcccgga ttgcgccagg gtgaggattt gtatttctgt ctgatgctgt tgactgttgc
     2281 tccgcggctc ctgatccttc ccgaccgttt gtaccattac cggagggaac gtcccggttc
     2341 cgcctcctgc gggcgggatc cccgcctggg ggatttccgc atggatttga tgggcatgga
     2401 atctctggcg cgtttctggg gggagcgggg gctgcttgat tcacccgccg ccttcggtct
     2461 ggtggactac tgc
//
LOCUS       DAWEMX010000219         2465 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13400_length_2465_cov_7.049793, whole genome shotgun sequence.
ACCESSION   DAWEMX010000219 DAWEMX010000000
VERSION     DAWEMX010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13400_length_2465_cov_7.049793"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          785 c          602 g          559 t
ORIGIN      
        1 ttcccaaaca gtaccggctg ccgttcttca tgctggtctc ctgttttgcg ctgtggggcc
       61 tcctgaacaa catgacggac aacctggttc cggcatttag caaaatattc atgatcaatg
      121 cttcggaatc cgccggagtt cagatttcct tctatggggc ctatgcggtg cttgccattt
      181 tcgcctccat ccttctggag gaattttcct ataaggcggg tgtcctcatc gggctggggc
      241 tctacatgat cggcgcgctg tgctacattc ccgccgccat cgggcaaagt ttcgatatct
      301 atctgatggc tatcttcgtg ctggccggag gcctctccat tttggagacc acctgcaacc
      361 ccttcgttct ctccatgggg tcccaggaaa cgagcgtccg ccgtctgaac ttcgcccagg
      421 ccttcaatcc cataggctcc ctgacaggta ttttcctggc caaatatttc attctggcgc
      481 atttgaacga tgcggacatg gacacccgca aagccatgga cccggaacaa ctgaacgcca
      541 ttgtcagcga tgaacttttc tgggtatgcg ttccttatgt gggcctggta gccatcgccg
      601 ccgtcatctg gttctttttc tgccgttaca aatccgacga tgaaaacgta cccacccggg
      661 aaaacacaca gcccctttcc tctaaactcg gccgcctggg actctgcatc gccctgattc
      721 tcatcccctt cctgttccag tactattccg ctttcgaatt cagcatggtg cagcaaatcc
      781 tgattatgat gatcgggccc atcgccttcc tgctcatcat gccggactac cgctgccagc
      841 ttatgaaact ggtgaagctt ccgcgctact tctgcggcgt ggcggcccaa tttttctatg
      901 taggcgtgca gattgccgtg tggacgtgga cgattaaata catcatgtcc gtcttccccg
      961 gaatgcagga agcggcggcc tccaactact atatcttttc catcatcctg ttcatcgcct
     1021 gccgcgccat cactacggcc ctgatgaaaa aatttaaccc ggccaacatg atgtcccttt
     1081 tcgccgtcgc cggcattctc tgctgcctgg gcaccatcta tctgcccagg gaaatctccg
     1141 tctggacact ggtcggaatc tccggctgca tgtccctgat gttccccact atttacggca
     1201 tcgccctgcg cggactgggg tctgaagtga aactgggagc tgcgggcctt atcatggcca
     1261 ttctgggcgg cgcagtcatt accccgtaca tgggttcctg gatagacaac accacagtaa
     1321 aaaaccaggt ggcctatttt gaatccatca accgtcctct gatcgcgcag gcggaagctg
     1381 cggacgcccg caaatcctta tccggagcca tgctggcgct gtacaggatg tcagaagcca
     1441 gccctgcgga cggagaagaa caagccaggg ccgccggcaa attccagaaa aacctggaaa
     1501 ccttcctttc cctcccctcc ctggaagggg acgcttttga gcaggcggcc aaatccctgc
     1561 ccggctgctc ctccctcaag cctggggaac tggccgtttt caaagccaat ctggaacgcc
     1621 tccccgctcc tgccggaaga gaacagcaaa ttcagcaatt ctccctgatt ccggctctct
     1681 ccacgttatc cggaaaccag gcggaagtgc tggacaacct catcgccctg cccctggaca
     1741 tgaagcaatt ccagacggcc caggaaaact tcgtggaaaa agcggtgcgg tcatccttct
     1801 ttattcccat catctgcttt gccgtggtac taatttacgg cttcttcttc cgcaacactc
     1861 atctgaaaaa ggaagaggaa gaaaagcctg cccaggcatg aaccagcttc acatcaccta
     1921 cgcaagatat gaaaacagca ttaccaacca tcggtatccg ccccacgatt gacggtcgcc
     1981 gcctcggcgt ccgggaatcc ctggaagatc aaaccatgaa catggccaag gcggccgccg
     2041 cccttattga ggccaacatc cgccacgcct ccggagaacc cgtcaaatgc gtgatcgcgg
     2101 acaccgccgc ctgcgcggac aaattcaagg ccaacaacgt cggagtctcc ctggccgtta
     2161 cgccctgctg gtgctacggc agtgaaacat ttgacatgga tccgttcact cccaaggcca
     2221 tctggggctt caacggcacg gaacgccccg gcgccgtgta tctggccgct gcgctggccg
     2281 gcctgaacca gaaaggattc ccggctttct ccatctacgg aaaagacgtg caggacgcca
     2341 cggatacttc catcccggaa gacgtagctg aaaaaatcct gcgcttctgc cgcgccggcc
     2401 tcgccgtagc caccctccgc ggaaaaggct atctttccat cggcggctgc tccatgggca
     2461 ttgcc
//
LOCUS       DAWEMX010000220         2447 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13509_length_2447_cov_8.743311, whole genome shotgun sequence.
ACCESSION   DAWEMX010000220 DAWEMX010000000
VERSION     DAWEMX010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13509_length_2447_cov_8.743311"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          457 a          679 c          760 g          551 t
ORIGIN      
        1 gggatccggg atgtggagcc ccaggtccgc gttcagtacg cctaccagcg tcacgttggg
       61 aaaatccaaa cctttggcga tcatttgggt gcccaggaga atatctatct tatgggcgcg
      121 aaactggttc agaatggtcc ggacggcgtt tttccgccgg gccacgtccg catccacgcg
      181 cgtgatgcgc gccgcaggga aagtgcggcg caggagttcc tccactttct gggttccgta
      241 cccctggagc aggatgttgg cggaccggca ttccgggcag gaacggggcg ggagggcttt
      301 gaatccgcac aggtggcaca tgaggcggtc ttccgtacgg tggtaggtca ggggcaggga
      361 gcagtgcagg cacgtgacta cgtggccgca gtccgggcat tggatggagc gtgcgaatcc
      421 gcgcctgttg agcagcagga tgacctgttc ccccttgtcg agacgccggt caatggacat
      481 cctgaggcgt tcggagataa cgttgggacc ggatttgttc cttccttccg ttttcatgtc
      541 caggatgcga atgagcggga gctgctggcc gtccgccctt tccgtcagtt ttaccaggga
      601 atattttcct gtcagggcgt tatggatgct ttccagggag ggagtggcag agccaagaag
      661 cacggcgcaa ttttccagat gggcgcgcag cacggccaga tcccgcccgt ggtagcgggg
      721 ggagctttcc tgtttgtaag aggcgtcgtg ttcttcatcc acaatcacca ggcccagatt
      781 ttggaggggg gcgaagacgg cggaacgggg accgatgacg atgcgggctt ttccggaacg
      841 gatggcatgc cattcgtcaa agcgctcccc gtccgagagg aggctgtgca gcactgccac
      901 ggaggaaggc agttctgcga aacgggattt gaaacgctga accgtctggg gcgtcaggga
      961 aatttccggc accatgatca gggcggattt tccggatttt actatttggg aaacggcctg
     1021 taagtaaacc tccgtcttgc cggaaccggt aaccccttgc agcaggaccg gttttttgcg
     1081 ctctgccgcg cacatggccg tgatttcttc cagcgctttc tgctgctggc tgttcagctt
     1141 catgggctgg gtggggacga attgctcccc ggtgctgggg tcccggtgca cggcctcttc
     1201 tttcagggag atgaagcctt tggcttccag gctgcgggca acgttcaggg cgccggcgcc
     1261 gaatgccgcc aggggggcgg actgttgttt cgcggatgag aaataatcca gcatttgggc
     1321 ctgccggggt gctttgcggt acagggcgtc cagttcctcc cgtgccggag ttttttccag
     1381 atacaccatt ttgcgcattt tttccgccgt attttcctga cgaacggttt ccgggagcag
     1441 acaccggatc atctgatcca gagccacgga gtaatagtcc gccgcccatg aggccagatt
     1501 catcatcacc ggggaaatca ggggctccgg atcgatcagt ttcagaatgg gcttgagcct
     1561 gtccttccag gccggggccg gttctgacag cgtaagcacg gttccggtag ccgttctgtt
     1621 gcgcaggggc acctgtacgc ggcagcccgg cttgacgtct cccgcttccg gaggaatacc
     1681 gtaatccagt accagatcgc tttgtccgtc aaccagaatg cgcgccgcct gcatggagtg
     1741 aaaaggagga taacattaac gatagccgaa ggggaaacgg gagacgccgc tcatgtcctt
     1801 cagaacttca atgtaattgt agccgtatcc atccatgatt tgcgttacga cggatgcctg
     1861 gtcgtacccg atttccaggg ctacaaagcc gtcttcattc aggtaatccc gggcgcgggc
     1921 cagaaagcgt tcaatgattt cccagccttt agggcctccg aacagggctg tatgggggtc
     1981 tttcattact tccggggcaa gtttttcccc ctctgcaatg tatggcagat tggccacgat
     2041 aacgtcaaag agcatgttcc gcccgttttc ccctttttct ccctccggag cagaatcggc
     2101 atcctccgca tgcggatccg ttttttcctg gggaatgttg gcgaacagat tcgtctggat
     2161 ggtggagact ctggctccca gccgcatggc attttccagg gccagatcca gagcctgcgg
     2221 ggaaatgtcc gccagcacga cttctccggc gcgctccttg agttccagcg ccagcgtgac
     2281 gccgataacg ccggagcccg tgcccatgtc caggatgcgt accggatggt ccgggatttt
     2341 ctttaacacc atttccacca gttcctcggt ttccgggcgg ggaatcaggg cgcgggcatc
     2401 cgtcaggaaa tccctccgga aaaattccgt gacccccagc agatgtt
//
LOCUS       DAWEMX010000221         2437 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13569_length_2437_cov_11.456759, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000221 DAWEMX010000000
VERSION     DAWEMX010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13569_length_2437_cov_11.456759"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          501 a          822 c          676 g          438 t
ORIGIN      
        1 gtggccatga tctctctggt cgtcggcggc gtcggcatca tgaatatcat gctcgtttcc
       61 gtcacggaac ggaccaagga aattggcctg cgcatggcgg tgggcgcccg tccgcaggat
      121 atcatgcgcc agttcctgct ggaagcggtg ctgctctgcg tggtgggcgg cgcgctgggc
      181 atcatgctcg gcaaggcgat ctccatcatc gtcagccgca ccatgaactg ggccacggcc
      241 tcctccccgg aagccatggc tctggctgta ggcgtctccg tattcatcgg cctggccttc
      301 ggatggtacc cctcctggaa ggcgtccaag atggacccca ttgatgccct tcgccacgaa
      361 taacacctta ctttttattt ttccatgtca tccacgttca aacatctccc ccttctggcc
      421 gcctgtttcc ttggctcctg catggtaggc cctgatttcc ggacgccgac caatgacctc
      481 cccgccacgt gggaggcgaa caggcccccc cacagttcgg acaaagattt gcgttcctgg
      541 tggaatatct ttggagaccc ccagctcaac cgcctggtct ccacctccct caacaacaat
      601 ccggacatga aggtggcttt gctccgcatc cgggaggcca gggaaaagct ccgcgtctcc
      661 caggcatccc tgcttccctc cgcagacgcc agcgccgggt ggagcctgtc cccggacaga
      721 ggttttcgca gctccacttc ccagaacttc tccctgggag cgtccacctc ctgggagctg
      781 gacctctttg gcggcaaccg gcgttccatc gaggcctccc gcgcctccct catgtccaca
      841 gaagcttccg caggagccgt gcgcacagcc ctgctggcgg acgtcgccac ggcctacttt
      901 gactggatta ccgcctgcga acagctccgc atcgccaggg aacaactgga aatccaacgc
      961 agcaccctca ccattgcgga aaaacgctac aaaacggaat tcgccccccg tctggatgtg
     1021 gagcaggcca cctccaccgt cgcctccacg gaaagccgca tcccgcaact ggaagcacag
     1081 gtggcctcct ctaaaaacca gctcgccgtg ctgctgggaa catacaattc ccgtgtagaa
     1141 ctgacgctgc ccaaagcttc cgtctttgag aaaacgccca ccgttccggt aggcctcccc
     1201 tccgaactcc tgcggcgcag gcccgacgtt atcgccgctg aggcggacct gcacgcggcc
     1261 gtggccaacg tgggcgtagc cgtagcggat ctgtacccgc gcttcagcct gacgggctcc
     1321 ctttccagcc gcggcggaga tttcggccag ctcttcaggg aaaacaacaa cgcttggtcc
     1381 ctgggaggga atctgctcca gcccctgttc cggggcggag ccctgagagc cacggtacgc
     1441 gcccagaaag ccgcagcaga acaagctgcg gaaacttacc gcaaaacgct cattaccgcc
     1501 gtctccgagg tggaggacgc cctgatcgcc tatggcagct atacctccca gatgcaatac
     1561 ctgcacaagg aaaacaacgc caacagagaa gcgttccagc tctcccgcac cctgtacatc
     1621 aacggcgaga cggacttcct gaacgtgatc accgcccagc gttcctggct cagctcggag
     1681 gaatccctgg tcaccatgaa gcagaacatc cgcaaggccg tggtgcagct cgcgcgcgcc
     1741 ctgggcggag ggtggtaacc agccgctccc tccaaagtcc ggggtcgcca tccccggcaa
     1801 acggaaccct gatttttcaa caggggacac ggcagcaagg gaatgactga agaatcattc
     1861 tttgtacccc ccacgttcag attaaaaaag gccgcaagaa taggaacgaa taaactatcc
     1921 ggaaacttcc gatggaattg aacagatagc cggggctcag gttaagaaca ctctccagcc
     1981 acttctttca tgactgcgct gataaggttc cgggtcttca cgccgggcag cacgttcaag
     2041 tccatgcggt tcatcgcata gccgaaggaa aggccgtggg ccggatccgc aaaggcatgg
     2101 gaaccgcccg ccccggcatg accgaagccg ccggagccga acagatggcg cagcttgcgg
     2161 cccgtggcgg ggtctgcggg atcgtgcatg aagccgcagg aaaaggcggt gggcgttttc
     2221 agcgtcaggt ccggcccgtc aatgacgacg ttccgcatcc agccgcgcac gtcggacggg
     2281 aatacccgga gttcatccag ccccaggcag gcctggtaga actgcgccat gccgcgggcg
     2341 gaagccacgc cgccaaaggc cggaactccg gacacccacg cttccggcgt attcatctgc
     2401 cgcacactgt tcagccctat cagagtattg aacgcac
//
LOCUS       DAWEMX010000222         2435 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13582_length_2435_cov_9.160924, whole genome shotgun sequence.
ACCESSION   DAWEMX010000222 DAWEMX010000000
VERSION     DAWEMX010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13582_length_2435_cov_9.160924"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          617 a          733 c          509 g          576 t
ORIGIN      
        1 aactcacgga agaacaggcc aaaaccctga aggttaacca gcaataaatt tcagcaagcg
       61 ctgtcggcat cattaaagcc ggacattgaa tttccaatgt ccggcttttc cgtttccagc
      121 ggggaacaga attccccagg acagcacgtc aacgcctgcc tccgggaaaa agaaaaagcc
      181 ccggacggct tctttccgcc tcagtcataa ttttctggcg ggaagagcag gcaggttgta
      241 aaaaaccagt gccatgaaat gtcttgcgtt atttctcatg ttcctgcttc agccggcact
      301 ggcggaagat acagccccat ggaaaatagc gatcatcggc gatacgcacg actcccccaa
      361 gcgtatggaa ggaagcgagg gagtagcagt caacttcatc aaaaccctct atggggaaat
      421 cctgaaacac aatgtggaca tggtcgtcca ggtaggggac atggcggata ttgaaggaag
      481 cgcccccgtc aagggactcg ccaaacgcaa ggaactcaac aaaattctga gagaaaaagg
      541 catccccttt tatgccgtgc gcggcaatca cgaatccctt cctttccggg cggaacaatt
      601 ccgggaactc ttcctgccta cgcggaagca gggcgccaaa gggctggcta ccagaaagtt
      661 gaactacggc atccgccata aaaacgcttc cctgtatttc atggatattg acctcacccc
      721 tgaccagctc gtggacttca gtgcgtgggt caaacgcaac agaagcaagg ccaacaccgt
      781 tccgaggcac tgcctggtct tcacccaccg caccctgcag acgcccatgc aattccggga
      841 atgcctgtgg ggccgttaca acgacagcgc ggcggaacag caaaacatct tttaccgcaa
      901 cctccgggat gccggagtgc gcttcgtcgt caccggacac ctgcacgccc atgacctgta
      961 tatgattacc tccccggacg gacaaaacac ccttacctcc ctgatctgcg cccctgcggg
     1021 gaacaaggtt cttcccatcc cttttctgct gcctgcaaaa tcccgggtaa aaaccctgca
     1081 ataccgctcc ggcattacgg cctactatat cctgaccatt tacccggatt ccatgaccct
     1141 ggatacctac gccgccccca ataacggcac tactgacgaa ggcccccaaa gcggagagtt
     1201 caacaaattg aactcctacg caattccgat ggattagaac atactgcggc ccccgtttct
     1261 tccataacgc agcatcccgg aatctgcctg ctcccgtccg ctatacaccc ctggcgtccc
     1321 tggcggaaca atggaatttt ccgggaagtt cccaatcctc catgttattt catatgcccg
     1381 gagccacggg actttatctt tctatcgtgc tggcgccccc ggtcgccctt atataaaaac
     1441 tccgttcttc catctcggcc tacagtacgg tcaatcaggt ctccgatttc ctggtcagcg
     1501 tacccggcct tcttaagata cttaaccact tcaccagcct gccccggata aaaacggacc
     1561 agcatcccca caaacgcagg ttccaatctc ttttccaaat gatacttaca attgttattt
     1621 ctgtcttcat tagacagcag cttattgttg taataaattg atcggggatc agccccgcga
     1681 aataaactca gttttcgttc ctgaaccagt ctcaaggttc tttcggcact cattttatcg
     1741 tattcctcct tcatcctggc ctgtttctgc tttaattccg gatcatactg agtcctgaat
     1801 gttttcaata cggccgctcc atattctgcc cagattctgc acagctcctt tctctttacg
     1861 ccgcatcggg aaacgattcc ggatatatcc caggataaac tgctcatcca cctcacaact
     1921 ccggtcttca attcataatc catgtttcca tagaggccgt ctttgggggt attttcatcg
     1981 taatcccatg aaaatgcatc cgccgaaact acaacataaa aaatccagga caacaccctg
     2041 acgtcttctg cgttcacatc tccagctccc ttggaactaa aggcgccaag tttcttccgc
     2101 gcttcccggt aacaatccgg cagctctttt tcatgcaaca gggaaagaaa ctccttcttt
     2161 tcctgaggag acgttttgaa tgcggagatg ctgtcgggca acggaagccg gagctgggca
     2221 ggctttcctt cagcagtatt tcccaaatat atatcagcgg aatatgcctc tgtttccgtt
     2281 tcaatcctca acgcgccgga atcctcttcc tccacgtccg caaaggaaaa acctcacagg
     2341 gaaaccgcgc aacctattaa aagaattctt atatttttaa caacaaccgc catcagacgc
     2401 agatgatacc ttattgccat ttcccattat tccat
//
LOCUS       DAWEMX010000223         2425 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_13639_length_2425_cov_8.910127, whole genome shotgun sequence.
ACCESSION   DAWEMX010000223 DAWEMX010000000
VERSION     DAWEMX010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13639_length_2425_cov_8.910127"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2170..2179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          466 a          535 c          715 g          699 t
ORIGIN      
        1 catcccagca gtttttccac cgcgcaggca taggcgatgt tgttggacag gggggccagg
       61 tagtcaaagc ggtcggtgta gggaacaaat tggttgtact ggatggtttc gcctattttt
      121 tccattccgc ggtgcagatg gccaaccacc ggatcacagc tgatgatttc ttcaccatca
      181 agttccagca ctaggcgcag cacgccgtgg gtggcggggt gggaggggcc cacattcagg
      241 gtcatcgttt ctccgagcgg ttcggatgta ttggtgagat agtctgcgtt agcggctgta
      301 tctgcaatgg cgaaagtttt ggtgctcgtc ttcatgtgga tgtccgtgcc tggctgaacc
      361 tgataatggt gccggatgat tatgccactt cacaccatgc gggcaagcca aatgtcagtt
      421 tttttggcat tccggggagg tggtgcgcga gtctcctcaa ctgatgaatg ttagtccttt
      481 ctaacaataa gcttttatat ggaacctgcc gaatatggga aaaagcggat taggaatccg
      541 gagtcaacct acaaatttga gaaaaaataa tttaatgcaa atttgctcag ttatttccga
      601 atggcgggtg ccaggaaaac agggctgccg ggccttatgg ctggacgtgt ccgaacgcag
      661 cggaggagaa tcttcttcct ttgtagccgg cctccctgcg tagtcctttt cgggattcac
      721 gttatatgcg gattcctaat ccgttaggaa aaaaaatatg ttgaagccga ggagaatttt
      781 tgtctttccc attgctgtaa cattttcagt attatttatt ttatatatta tttcatggtt
      841 ttcccctatt gccggggatt cctttattca cgacaggacg gggtaccttg ggcagtttca
      901 tatccgccat gtctggaagg cctgcgtgga cagttacctg tactggaatc ccaggctggg
      961 ggaaatggct gcgtttttta ttacttctgc tccccggctg gtctggacgg tcttgaatcc
     1021 cgtgtttgtg cttgcccttg ttcttggcct ttatgtgctc gctctgggga gaatgcccaa
     1081 tctccggcgg gagtgcggag catggacgtg gttgtttgct ttaagcatgt tcgtctcagc
     1141 cggggtaacc gtttattatg tctgcctgac acgcgcaggc tccatgaatt atgtgtggac
     1201 cggatgcctg atcgtatggt tcatgaatat ttacagaacc aggtggggga agcggataac
     1261 ctcccctcga tggggcctgt cctcaggatg tctgatttat gggattttct gcggcgcctg
     1321 taatgagggg gcgacgatag gaatggcggc ggcgttttgc atcatgaccg cggtcggcat
     1381 gctccgggac cgcagggttg gcgcttatgt atggtgcggg tttgccggcg ttgcgttggg
     1441 aggcctgttt ctatttgccg cccccgggtt gtactcgcgg cttggccatg atttgggagc
     1501 gagtgaagtt ttgaggaagg gcggtttttg cgcctatgcg gagtctttcg ggatattgct
     1561 ttattaccac gcgaggatgc tgtgtaaaat atatgccgtt tccgcagtat taacgggatt
     1621 tttgttgttc agcggcacgg agcggtgccg ggaaataatc aggaaggaaa aggaatcctt
     1681 cttttatatt ctttttttat tgttgctggg gggcatcatg tccgtttgtt attttccggt
     1741 gtgcattccc gcccatcatg ttcagttcgt gtcgtccctg cccgttttgg cggcgtcaac
     1801 cgtgttgttc tccgtattgt accggaatga gatcatggtg cgcggattgc gctcggtgac
     1861 tgtttgcctt gccggagcga ctttgttttt cggggtggtg ttcaccctgg agtattatca
     1921 tgtaggctct caggctttcc agagagagga gctgattcat aaggccttcc aggaggggaa
     1981 tctggaaccg gaagttcctt ccatctctcc tcttttcttt gaaggaaaag tatccggaac
     2041 ggatggaaat tggggagggc cggataccaa ccgccggttt ggcatccgca agctggtggt
     2101 gaaaaagccc tgagggaaaa tggctatttg actatgttcc ggggagtgaa gtgcatttcc
     2161 tgttcctcgn nnnnnnnnnt cctcgatgcc gaattgccag gtggccgttt gccacatgtt
     2221 gacagcagga tttttccaac ggacctggac ggttacgggg ctgagccgca ggggacggga
     2281 gggagtccat ttcaaaattt tttccttggc gtcgtaccgg gcgtttacca ggccgaagcc
     2341 ggagacgcgc atttccattt tcttgggttc caaggagggt tcccctgcca gggaaatgga
     2401 tatatcggga gtctggtttt tcacc
//
LOCUS       DAWEMX010000224         2367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_14013_length_2367_cov_5.426903, whole genome shotgun sequence.
ACCESSION   DAWEMX010000224 DAWEMX010000000
VERSION     DAWEMX010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14013_length_2367_cov_5.426903"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    394..403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1361..1442
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          511 a          594 c          594 g          576 t
ORIGIN      
        1 tcaaagctgc cgccagccat gagggtgccg gaggtcagga tggcggggac gtccttcagc
       61 cacagcgccc ggcgcagctg ctccggcagt tcccggctgg cggcgcagag gctggggctt
      121 ccgtccctgt cgtactggat gtagaggatg tattttcggt ccccggtaaa aaacaggccg
      181 agggcctgct ctgttgttcc cagccggtaa agaagccacc gtgggaggtg cggggcaagc
      241 tgtttttgaa gctgccgaag gagggaaaga caatctcgaa gggccgcttc ccgatccggc
      301 gttaaaataa aggcggtgcg ctgggcttct tccagcagtt cgccccggca catggccccc
      361 atgagagcgc ggaacttttc ccgcagacgc tggnnnnnnn nnnttcgcag cgaacagctt
      421 ttctcacaaa ccttggggac gcatacctgc cgcctgtcaa atccactgag gccggttacg
      481 gcgtctaaat cataataggt gcgaagggag agcagggctt tcatctgctc cgcattcttt
      541 ttcttatgtt tcacggcttc caaccgatga gaaagacgga catcgcagac aaagcgttcc
      601 ttgccttttc gcaccatcgc ccggatgggt ttttgaataa tgcggttctc cagaaaaatc
      661 cgggacaaaa atgggatgta ctccccgatg atggcgtcct gcagggcaac gctggaggtg
      721 gagaccacta ctggctggga accggcaggc ccgtgcgggg caaacttctt cagtaaaatg
      781 caggcggtca ggtaggcgta ggtcttgcca atcccgactc ccgcgtcgca taaagcgata
      841 ttgtttttga gcagggtgtc cagcatggcg tggcataggg tgatctgttc ttcccgaact
      901 gccagtccgt tttgagggag aaggattcgg aagatccgtt cgatttcccc atgggcctgc
      961 tctttcaggc tattgttcat ttcggcacat tctccttaga gcaatttgct cttgtgcgtt
     1021 ggcggcaaac tgatgcggcg cagaaaacct gcgccgcacg gattcgccgt caacggcaat
     1081 ctgaataaaa ataaaagggt tgaatttgat gtgccgtatt tgctgctcgc cccccggcaa
     1141 ccttgggaac catgcgggca ggcttgcagt tgacaagccg ttcactggct ccccgaaggt
     1201 cttgctgatt acaacccaag gtcggccagc ccatctgacg tgtgggcaaa acgatagcgc
     1261 gtatcataat accccgccca aggtcgtggc gaaaagggaa actaccccct ctttccggtc
     1321 aaaatgcatc gctcaaagcc aacaacacaa gccgccatcc nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nngccggttt tctctgagat ttccttgctg ccgtatccgt ccatcttcat gaagaggatt
     1501 tcgagggtga gcttgtccat attgtgtagc aaagaaaaaa gctccatatc ctcgatgctg
     1561 tccagcaggg cttcggtatc ctcgacgggc agttccagat cctgtgctga aacccagtcc
     1621 acataatgtg accattctac ctgtcgctgc aggtactgcc gttccttatt gaactgcgcc
     1681 caatcgtatg tatgcaggcg ttggatggtg tcctcatcca cgccgagttc tctgagtttt
     1741 ctttcttctg cttctttcca tttgagccac tgtttttcgg ctctggcttt gttgtaagcc
     1801 atggctgttt cctccgatca atctgaaatt ttgaaaaatt cagattgtcg gggcggcggc
     1861 ctgccgctat gagttcttag ccatgacctc tttctaaaaa catgaaaaaa gcgcacccgc
     1921 ccgcaggcga atgcgcatag cgaggctgtt tcctcgaaat tggcatttaa tggtaaatta
     1981 aaatgagata atgtcgaacg aaaaagggct gcagcttcgt atgaagctgc agccctttct
     2041 gtgtgcggtg gttttcgccg cattgtcgcc atcccctctg catggggaaa gcggacgttg
     2101 ttttatttcc atatttcccc atataaattt agatggggac agtataaaag agaaaaacta
     2161 cttttctgat tcgattctcc tttcatttgc atatttcgta gtgcttttat ctacattaca
     2221 tgacaaaaga acaagaatat ttatttaatc aaccaaaaag gtaaaacaac agcctttttt
     2281 ctctgcacag gtaccttcaa cgatccggca ggtggggttc gatttattca ggccgagttt
     2341 ttcctgcagg cttcgcacat aggcggc
//
LOCUS       DAWEMX010000225         2286 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_14518_length_2286_cov_7.897355, whole genome shotgun sequence.
ACCESSION   DAWEMX010000225 DAWEMX010000000
VERSION     DAWEMX010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14518_length_2286_cov_7.897355"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          505 a          525 c          707 g          549 t
ORIGIN      
        1 acggcggatg tggaagcgga ataccgtccc aaccagcttt ttgtgcaaaa ttgccggatg
       61 gaaatggaaa agggcgtctt tgaattgttc gccaacggtt ttctggacgg tcagatgttt
      121 gtgatggggc agtccacggt tccgctgcaa acgatagacc aattgttgag catggaggat
      181 gacgactttt tcatgaaccg ctttgtcttc cgcaaggatt ccggcctgga gttttccttc
      241 cagggaacgt tgggttgtac aatctggaaa aggcttacga cttgcaggct actgtttccg
      301 caaccaacac gcgctacagg ggggtggact tgaagtctgc ccgggcggat gcccatctgg
      361 tgacggacca gctggtgctg accaatgtga cgactgtggt ttccaacgga aattacctgt
      421 cttccgtcgg attgtccgga ggcccttcgg aatgcactct cagggccaag agcatagatt
      481 tccgttttgt gcaggatacg gtggaagtgc ttggtctgga ggggcaggcc tatcccggct
      541 ataccctgcg gatgttttcc gatagtgccg ccagggtttt gaaggagttt gtctttaccc
      601 ggcccgtaac gctttccggc ggcggcatgt ttccgatggg ggatgacatg aagctgatga
      661 aggggcgcat ccgctttgac gcttccgccg ggcgcgtgcg ctatcccctg ctgggaacca
      721 cgctggacct cggaaggaca aagggggaag tgttgatttc tccgcaatgg gtggttgtgg
      781 acaagatgat gggaaccatc tgggacggaa ctttcacggg ccgcattctg gcgcagattg
      841 atgatgggga tgccctgaac ggttcttttg tcctccagga catgaatttg acgtctatcg
      901 ggaaatccta tgacaaaaaa atggagaaag ccactgtgca cggagccatt gagttttcat
      961 ccaagggagg caatatgaat tctattcagg cgaaggggga ggctgccctg gtgcatggcg
     1021 acctggtgga aattcccctg ttcggatttt tgggggaagc cctctctaat tacattccag
     1081 gactggggca tctgattaat tacaagatat ccagggccga ttgcgatttt tccattgaga
     1141 agggttatgt gcgcaccagt aatttcgcgg cccagggaag caacatgtct ctggaggggg
     1201 gaggctggat acgcctttcc gatttgcagg tcaattcaga tttcaagctg gggcttcgcg
     1261 gtttgccggg gtttattact tccccggttt tcctgttggc cggagggctg tttcaagtgc
     1321 ggggaacggg gcctttgagc aatgtttcct ggaatttcgc tccgttttcc ggagggaaag
     1381 cccccgttcc tcccgccgcg gcgcctgccc ggaaaaggta gggcagacga aatatttggg
     1441 tgttgcgcaa ctgccgtaag gagccgcatg ccttgcagca attgaaaata acagataccg
     1501 gatatctgac agaagataaa gagctgtaaa ttcagaatgc cccgtttacg gtgctttccc
     1561 tcaggaaaag gagttcccgg agaaattttt cttaaaccag agtataggcg gaaacagctg
     1621 taatgctcaa tttctgtagg aaagaccagt gaaacaggag gtttttcccc gggaatgaaa
     1681 cccggggctg aacggtgccg gagcgtaaaa acgtatgaac tatggagccg gaaggaaata
     1741 tccccaccat gcaatcctga agaaggtgca ggagttcctt catggaaaag acaggctgcc
     1801 ggtttccgtt gaagtgccgc gatttgcttc ggccgttctc ctatgtgaac gcccgcctgc
     1861 atgttccata cgggtagatc gtgcggtccg gtacgctgct ggctggatgg gaataagccg
     1921 attccggggc cggatcggct gttcccggca gggcggggcc ataatggctg aggagaggat
     1981 gtggctttac cggaaatagt ctgcggcagg agaggcggtt ttaacgggta aaaaagcaaa
     2041 taaggcagga ggtgttggcg taaaagaagg aagtttttat tccggagtgg gtccgttaaa
     2101 aacggggcgg ctgaactgct ccagccaccc cgttgaaaaa gggagttatt taccggattt
     2161 tcccagtttg tacccggcgt aggtttccgt gttgtacagg ggctggttgt ccggcacggc
     2221 gtaaccgatg gatttgcgaa tggcgaggaa gtccgccacg ccttccggcc ccacctggtt
     2281 cagagg
//
LOCUS       DAWEMX010000226         2162 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_15395_length_2162_cov_8.177504, whole genome shotgun sequence.
ACCESSION   DAWEMX010000226 DAWEMX010000000
VERSION     DAWEMX010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15395_length_2162_cov_8.177504"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          455 a          512 c          605 g          590 t
ORIGIN      
        1 gagaacatga taggacggtt atttgtttcc catttggaaa attgcggaga aaccgtgtgg
       61 tcttctattg caccctcctg gatttgatgt aaactttttt attatgggaa ttgatgtaat
      121 atacgccgcc tttcgggccg acccggtagg ttctgttttc ccgcgcccgg agggcccggt
      181 gggcagtgtg ttctcccggc accattccgg aacacagagg cgtatattcc ggaaggagcg
      241 gtttgcggct gcattgggga aggcagaaga gaaccggaag gaggacggcc tggatgaaaa
      301 gggagtgttt cacaagagcg ttactatcct tttcagaggg ttgttttgtc aagtgggaga
      361 gcccggctga acggcttttc gtgaaaagaa tatgattttt tatgggttat ttgaaaacga
      421 gtgtttgaat ttattttatt tccaattact gttgctgaag ccgctgctgc attttagttg
      481 ttaaccgctc atttatccgt atccgctcca tgaatttctt tcctcatatt cccatgttgt
      541 ccactgtatt caacagtata ggttgctatt tattgccgtt cggagttaat ttgctgtggc
      601 ttttgtacga gacaaaaaaa gtaaagaccc tcctccttgc cgcgctggtg cttttgagca
      661 tagtatgcat gtttttccat gaagccttcg ctcccagcag ggaatatccg tgggactgca
      721 tggtggagca tatttttacg ggaacgcttc tgcctttcct ttgcctgctg ggatatcttc
      781 tgtacgtgtt tctttgcgcc tgcattcctg tttaccggaa ggtgtggaag ggcatcggtt
      841 ttttcgttct tggaggaatg agcctggctt ttgagtacgt gaatgtgttc ttcccggtgg
      901 cgctgctggc gggttattta ttccgtttca ccattttccg gaaattcagg aatcaccttc
      961 ccctggtcag gaaatgtcac ggggttcttg ccgtaggggt cattccggga atctgtctga
     1021 tcggtgttgt cagcgccatt ctgtttcctc ctccggagag ctttatatgc gacaggattc
     1081 atgaggaaat ggagcgtgac atggctttgt ggaattacca gagcaatctg gtactgctgt
     1141 ccagccgttt tcttaatatg gaacgcgaaa acggctgtat taccgtaaag tactattgcg
     1201 aaacgaatat gggccctgcc gtgtacaagg cgttctgcaa taattacggc ggttatgtgc
     1261 gggcggaatg ttccctgctg aacagataat ttccctgatg ggaaggagtc cgccttttca
     1321 ggtcgaagct gtttagatct gaatgtctcc ggtgaacaca aggtctgccg gtccggtcag
     1381 cgtgacgtca gtaaaacggt cccctgtgcg ttggaatccg actttaagcg tgtctccgcc
     1441 cgccacgtcc acctgaatgg gggagggggc gtccgccaat acggcatgca gcagcgcaca
     1501 ggcggtcatt cccgttccgc aggcaagcgt ttcatcctct acgccgcgtt cgtaagtacg
     1561 tatggcaatg tgccccggag acaggattct ggcgaagtta gcattggtgc ctttgggtga
     1621 aaaggcctca tggtaacgca gataggctcc cagcctggaa atgttcaggt cctccagctt
     1681 gtccagaaac gcaacggcat ggggaacgcc cgtgttcagg aaatggacat ccgcccccac
     1741 agtcggatcg gcattcagca cgaagagctg catggagtgg ggattggtca ggtttacggt
     1801 gacgttgccg tctttttcaa aggttgcggt gacgacgccg gccatggttt caaaaggaag
     1861 gggggctgtc ttgtcgtgat gaagcagggc ggcggcaaag ttgccgaagc agcgggcgcc
     1921 attgccgcac atttccgctt ctccgccgtc cgcattgtaa taacgcatac gaacaattcc
     1981 ccccttgccc tgggaaggtt ccacggcgat caacccgtca gccccaatgc cgaagcggcg
     2041 gtcacacagg gcttctatgg tttctttgtc cagcacggag gaaaggctca ggccacggtt
     2101 gtccaccatg acaaagtcgt tgccggcacc cgtcatcttg tagaaatgaa gcagcataat
     2161 ag
//
LOCUS       DAWEMX010000227         2109 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_15835_length_2109_cov_9.864167, whole genome shotgun sequence.
ACCESSION   DAWEMX010000227 DAWEMX010000000
VERSION     DAWEMX010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15835_length_2109_cov_9.864167"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          669 c          589 g          422 t
ORIGIN      
        1 gcggtggagg aacacattcc ggatgcagcc ccggtggcag gtcctgcgcg ccacatcgtg
       61 cgcacgggag atacctggga acgcgtcgcg cgtgacaacc aggtgcccgt caatgacctg
      121 aaagccgtca atcccaaagt tcagcgcctt gtcagcggaa gcacgctcgt cattccggcc
      181 cggccaggag acaaatttga accggaaaag tccaccgcgg aggaattgga gccggaaggc
      241 gtgccccaca ttgtgaagaa aggggaaacc ctctccgtaa tcgcccgcaa atacaaaatg
      301 aactggcgca cccttcagaa attcaacaag atgaatgacc gggacgtagc gcgtctcaaa
      361 atcgggcaaa aaatcatgat ccccaaaaag taggcctctt cctgcccttc tgccgccatg
      421 tcttccaaag tctgcatgat tctggtctgg tttttcgtca tctgccttct ggtcgtaggc
      481 ctggtgatgg tatccagcac ggcggcgtgg gcggaggaaa ccaagcaccc gtacgaaccc
      541 ctgttcaagc agaccgcctt tgcctgcgcc ggtctggtgg gggccatgat tctctcccgg
      601 atagactacc ggatatggag gaagtatatc tggtggatcc ttgggggggc ctgttttctg
      661 ctggtgctgt gctatgtgcc cggcatcgga aaggaaatca acggggaacg ccgctggatc
      721 accatcggca tgcagttcca gcccagcgaa tgcgccaagc tctgcatgat gatggccctg
      781 gccaactggc tggccctgta ccgggaccgc gccacctctt tctggtgggg ttttgtgatg
      841 cccggcctta tcttcggcat tcctcttgcc ctgattcttt ttgaaaagga catgggcacc
      901 tccgtagccc tggccctggc cgcgttctgc gtcatgtttg tggccggaac gcgcaaaatt
      961 tacctgggcg gggctttcgc cctggccgga acggccctgt acgttctggt gcagagcaac
     1021 gccaaccgcc tggaacgttt cctggcctgg aaagacctgg acgcccaccg cctgggcgcc
     1081 ggcctccagc agtaccgtgc ttccatcgcc ctgtcccgag gggggctgga cggggtgggc
     1141 ctgggaaaca gcgcggaaaa acacggcacg cttcccttcg cccacacgga cttcattttc
     1201 gcgccgctgg gagaggaatt cggtttttac gggaccatgt tcgtcctgct ctgctatttc
     1261 ctgatgacgt atgccggcat cggcgtggcc atgcagtgcc gggacaccta cggacggttt
     1321 ctggccgtgg ggattgtcgc catcattttc tgtcccgcca tcctgaacat cgccgtggta
     1381 accaacgccg tgcccaattc cggcctgccg ctgcccttca tcagcttcgg gggcaccaac
     1441 ctggtcttta cgctggccgc cctgggcatg ctcaccagca tccagaggtt ttccaccggc
     1501 gcccagccca attgtgaaat cacccgcaaa gacgaacgct ccattgacgt aagattatga
     1561 ctgaaccctc tctcaaacat ccctccctga acatcgtgat cgcctgcggc ggcacggggg
     1621 ggcacctttt tcccggcata gccgtagccc aggaacttaa aaagcgcggc caccgcgtca
     1681 cgctcctcat ctcccagaag aaagtggacg cccaggccag caaaaattac ggggatcttg
     1741 acttccgcac cattgaggcc atcgcgatgc ccaaaatccc atccctgtcc ctgctggggt
     1801 tcggcgtcag gctgtacaaa gccatccgtt tcagccgcca tcttctggac gaggtggaag
     1861 cggacgtcgt catcggcatg ggaggattca cttcattccc tcccgtttac gccgcccacc
     1921 gcaaaggaat ccgcacctac gtgcacgatt ccaacgcgct gcccggaaag gccaaccgca
     1981 tgaccgccaa atgctgcacg aacgtgctgc tgggcattga ggaagccagg cactactaca
     2041 agcccgccaa atccattgtc accgccactc cggtacgcca ggacatggtg gcccggaacg
     2101 acaaacacg
//
LOCUS       DAWEMX010000228         2032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_16502_length_2032_cov_11.247344, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000228 DAWEMX010000000
VERSION     DAWEMX010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16502_length_2032_cov_11.247344"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          405 a          478 c          649 g          500 t
ORIGIN      
        1 gtaaaatgac gtcgtccagt ttgatatacg cggagcaatg gccgcacctg gcggaaactg
       61 ccgtggcggg gacggaggtg cttttgcggc aggagaagca ttccacctcc cgggcaggcg
      121 ccgggctgtt ttccgtagtt ggcgggagtg tccagggcgg tgccggggag gggtggctga
      181 ttttcccgga gggcgcggtg ctgatccggg ggatgggatg gaaggtgggg ttcatggaac
      241 aaagagggcg gcaaaagaac ggttcgttct tttgccgccc ctgaaagtag caggcggaga
      301 gttttttatt ccgcgtcaat atccagcgtg ctctggctgg ccggagcatg ggtttccggc
      361 tccggagcga aggcatggac ggaagccgcc ttcttggcgg cggaagcccc tgtggttcct
      421 accgtggggg cgcctacggt agaatggccg atgaagattg ctccgccttc aatagccagg
      481 gaggcggcct ggatgtcacc tacgagttcc gccgtgcttt tcagttccac gcggtccgtg
      541 actgttacat tgccgatgac cttgccgtgg atgatgacgg atttggtgtt gatttccgcc
      601 cggatgacgg cgttctggcc tacggaaagc accccttcgg aggaaatgtc gccttccacg
      661 gtgccgtcaa tcagcaggtc atcggaaaaa cgcagggaac cgattacctc cacatcggag
      721 ttcagcacat tgcgggtcag ctgctggaca cgggtggtgg gagcgtagga aggcgcggct
      781 tccgcaaccg gttcggggat gtcctggctc ttggctacat ccatccagct gggggtggat
      841 tcaggatttt tattgccctt gggggcggga acggtagtga atgatccaaa catggcgtta
      901 aaaagagggg gtgaaaggta agatgggagc tcttgaaact cgtccggttt aatgtacatc
      961 acccgcatgt ggatgtgaaa caattttctc cggcagggat aaaaaaatgt gtctgttttt
     1021 tatcgttgcg ctgccttggg cgttccggta gtgtgtgacc gtctttccgc gattgttttc
     1081 tgtggatatt ttcgttttga aatgagtgat ttatttgaaa aaaagctgtt cctttccccg
     1141 gccgcgcgca ggcacggcgg aaaagcgggc tttgcgctgg gccgcattct ttccgtcatc
     1201 tttttcgtga tcgcgctggg aatgtttttc ggcggtcttt tcttctggct ccagaagcgc
     1261 caggcatccc tggattccgg agcggccgcg cggatttgcg ccacgccgga gatgttgtcc
     1321 ggagaattga aagccgctct gaaagacagg gtggactata gtttctggcc tccttatcac
     1381 atttctccga tggaaaagca ggacctgctg cgcaactgcc tggcggaggc acagaaatat
     1441 ctgagcgccg cggatgtggt tcaaaaatcc tcttttgtgt ggaagtccct gcaatcgctt
     1501 cctttaatgg tcaggcatta cgccatgtct ccgcctgagg cgattgtcag caggcctaag
     1561 gggcctaaaa gctttgccgt gtttgaggtg gaggggcatc cctgcgccca gctcctggtt
     1621 ggttttgaaa agactcccga tatggaattc tgttttttca aggatgaaca ggatggttcc
     1681 tggaagctgg actggcagca gtttgcccgc tttcagccga tgaactggga ggattttgta
     1741 cggggaaaag gggaggatat tgcggaattc cgcgtatgga tggttcggga gcgcatgtcg
     1801 gaaaataagg atgactatgc atttaagctg attgctcccg ggatgaacgg ttccgaagag
     1861 cgcagcattg cccgtcccat ggtgtatgtc cccagaaaat cggaaatggg caagcgtttg
     1921 ttcatgttgt ttaagattaa tgaggagatg gaacattccc cataccgggt gttgaacgcc
     1981 aatgacgagg ctgacatgct ccgcgcgcgg gttcttcttt cccgcagcaa ag
//
LOCUS       DAWEMX010000229         1993 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_16895_length_1993_cov_6.486584, whole genome shotgun sequence.
ACCESSION   DAWEMX010000229 DAWEMX010000000
VERSION     DAWEMX010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16895_length_1993_cov_6.486584"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          469 a          482 c          580 g          462 t
ORIGIN      
        1 taattgcgtc cgggattatt cacatccttc acatgcacgg cggacatttc catccctgcg
       61 taaaacagga agatgctggc tgccagaacg aggttgttga aattgctcat gtccggcacc
      121 agcttgtccc agctcatatc cagcaggatc gggttgccgc cggcccaata gcccaggccc
      181 agcaggatca ggatggctcc gggaataatg gttccgatga tggttcccca tttggtgacg
      241 cctgcggaag tcttcatgcc gcgcagatta agcagggtgg ccgcccagta cacgcacagc
      301 accacgatga gaacatacca tttattggcg gcaagcgctt catcccatct ttgtccgggc
      361 cccatgaaag ccagggaaac ggcggcgaat gtcagaacgg tagggaacca gatggtactc
      421 tcaatccatt gcagccagat ggccaggaac ccccactttt tcccgaatgc ctcgcctacc
      481 cagcggtaaa cgccgccctt ttggggccat ccggtagtaa gctccgcggc gaccagggaa
      541 acaggcacca gaaagaatac tgcggcgaaa atgtaataaa aaacggaact cagtccgtaa
      601 gtgctttccg caggcatgcc gcgcaggctg acaatggcgg aaacgttgat catggcgaga
      661 gtgaatactc ccatgcggag gacggatttt accgccggag cgttattggt tgttgcgggg
      721 cctgttggag cagcccctgg ttgagtcgta ttattcattg gtttattccg ggttgagaat
      781 tgggacggcg taaagaaagt gccgacaccg gccttatctg gacgaccagg cgggagaccc
      841 tttcgttgaa gggatgatgc agatagaacc gccggagttt gtcttaatgg aggccgggaa
      901 atttttagac cttccggatg gaatatcctg gccggcagtg aatggccggg agttgggaaa
      961 cgcgtcaaca catggttatc ccttatgaat tttcaagaaa gatatatatt ttataaaata
     1021 gataagaata ataaatacgg ggaagcattg gcttcccgat atttattcct taatatctaa
     1081 ctatttggat ttcatgaaaa aacggcagtt tcagcatcat gtggattctt tgcctgataa
     1141 tgctctctca gtgtccggag gtgggccggc tccgtttgac aaggaggccg gggcgtttag
     1201 gggcagctgg gactggggga ttattgttga tccgctcggt ccgggggaaa cggcagagtg
     1261 ccgtttaatg ctgggggtag atgataacgg tactctgacc gttgacggtg aaggaatttt
     1321 cattccaggg gagggtaaat accatggagg cacttatagg gaaaaaagct tgagctttcc
     1381 cattgaaccg ggaccgcacc gtgtacacat gacttatgag aacgttgctg ttcctcctga
     1441 atggactaat ctggcaatcc ttaattattc cattgaagtc atggtctcgg atggcacctc
     1501 ttcatcctcc tatcagccgg atgaaggtta tccggaacct gtgagtacgg aagatgaggg
     1561 agaagacgtg ccgtgtaaat gcggggggga cggcaatggc tctcagagca attccggttt
     1621 tccctgtccg gaggaagaca acggtgaagg ggataacggt gaagacgagg ccccctgtac
     1681 ctgtgaaaac aatgagggcg gcagcagcag ctcttcggcg cacggcgtgc gcggttcctc
     1741 caccctttat ggctcgtcgg ctgccggcag gcgcgtggtc gcccggacgc tcaagacgga
     1801 aatggtctgg cgtaccaatt ttggctcttt ccggggcatg acggggttgc cgcaggggtt
     1861 gctggacata gtagggtaca cattttcgtc tgaactctgg tcccctcgtg cgctccatta
     1921 ctggcatccg atgactcatg aaatcatttc ggtgccgctg tcgggaattg gagcgaatac
     1981 cgctttccaa atc
//
LOCUS       DAWEMX010000230         1955 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_17263_length_1955_cov_7.176316, whole genome shotgun sequence.
ACCESSION   DAWEMX010000230 DAWEMX010000000
VERSION     DAWEMX010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17263_length_1955_cov_7.176316"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          488 a          554 c          511 g          402 t
ORIGIN      
        1 catgagcaaa aggattttga ttaccggagg cgccggcttc atcggctccc atctcagcga
       61 acggcttctc agggaaggcc atgaagttat ctgcatggac aatttcttca cgggcagcaa
      121 acaaaacatc ctccacctga cggattatcc cggatttgaa gtaatacgcc atgacgttac
      181 cgttccctat gtcatggaag ttgaccaaat ctacaacctg gcctgccccg cctctcctcc
      241 ccattatcag tttgacccca tccacacaat gaagacctcc gtgctgggcg ctctgaatat
      301 gctgggcctt gccaaacggt gcaaggccag aatcctccag gcctccacca gcgaagtata
      361 cggagatccc atggtccatc cccaaccgga aacctactgg ggaaacgtca accccgtggg
      421 cgtccgctcc tgctatgacg aaggcaaaag atgcgcggag accctgttca tggactaccg
      481 ccgcatgaac ggggtggacg ttcgcatcat ccgcatcttc aacacttacg gaccgcgaat
      541 gaaccctaac gacggaagag tagtctccaa cttcatcgtc caggcgctga agggggaaga
      601 catcaccatt tacggaacgg gcaaacagac ccggagtttc cagtacgtgg acgatctggt
      661 ggaggggatg gtgcgcatga tggatacgga aggtttttcc ggcccggtca acctgggtaa
      721 tccagaggaa ttcaccatgc tggaactggc ggaaaaagtc attgaaatga caggctcttc
      781 ttccaaaaca gtattccgcc ctctgccctt ggacgacccg acccagcgca aaccggatat
      841 tcggctggca aaagaaaagc tggggtggaa gcctcatatt acgctggaaa aagggttgga
      901 aaagaccatc gcctatttcc ggagcattct ctagaaataa ggtaagtcaa tctcagcggc
      961 tgctgacaaa atttctattg catgccatgc cccacggcag acagcccgga aattacgggg
     1021 ccattcattt accgggggtg cggcatgcca aacagccgga tattacccgt ttttgactgg
     1081 ctccgaaaga acggctccgc tagcataaga tgttatggaa catgccagca acaaggaaat
     1141 ctattccgtt caggcaaccg tccgcactta cgaaagcggt gcgaatggcc tgatgaaacc
     1201 ggaaacggtt ctgcactggt ttcaggaaat agcggaagcc catgcctcct gcctgggttt
     1261 cggctatgat ttcgtaacgt cccgcagcct ggcttgggtg gaagtgcgca tggatgcggc
     1321 cgtcagccgc cttccccggt ggaaggaaac ggtggaactc cgaacctgga cggcccagga
     1381 aacgcctctg ctggcgcgcc gcaacctgga aatcagggat gcccaaggca actgcatcgt
     1441 tgcagcaagc tgtctgtggg ctgtcattga catacgccgc aggcgtcctg tgccattaaa
     1501 caggcatatc agctctttcc cgaatacccc atgcaaagaa acggtggctc cggtatccat
     1561 ggacatttcg ggacttctgc cgatgatcag ggaatggacg gcggaacgcc gggatacgga
     1621 tttcaaccgc cacatcaaca atgccgccta cctggtctgg gctctggact cccttccgga
     1681 ctcctggcta aagaaccacc aactgacggg aattcacctg catttcagaa aagaaaccca
     1741 tgcgggagat tccatgaaat ccctgctgtt cttccggaac tccctcacct cccaccacct
     1801 catgcacgga gatgaactgc gggcggaagc ggtattggaa tgggaaaccg ctgaaatcgc
     1861 ataaaaaagg ccctattgct cgcgttccgt tcttttccga ctccggacag cggagagggc
     1921 ttccggaaac atttggcgcg tttttacaga tttga
//
LOCUS       DAWEMX010000231         1912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_17714_length_1912_cov_7.178244, whole genome shotgun sequence.
ACCESSION   DAWEMX010000231 DAWEMX010000000
VERSION     DAWEMX010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17714_length_1912_cov_7.178244"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          562 c          490 g          427 t
ORIGIN      
        1 gctttcccct cctcttccac gtaataaaaa aagctgcccc gcacggggca gctgtcgtca
       61 acttcatgaa tatggatttc tccgctattt catgaagccg ttaaaagcag aaattggttt
      121 ctatgttttt caagcgttcc ctgacggctt ccggcacggc ggctttttcc tcctgcgtat
      181 tgtaggggcc ggaatacaga acctttcccg cagagtccgt cacatgaact gtggttcccg
      241 tccgggaatt ggaagaaacc acgatagtcc cgttggcatc cgacatccgg acggaagaca
      301 tgctctggcc ccctccggaa accttgccca tgggaggcat gggcatttga tgaagtccct
      361 gcaagctccg gagcatttgc gccgcgccgg gagtcaggtt catccggccg atgatccatc
      421 cggtaatcat ccatggcgga atccttcaaa aactcatcca tcatctgctg catatccaga
      481 atacgctggg ggagattagg ccgtgcagcc attccggaaa aaggcgccgt ctgacgccgg
      541 acggaaggaa ctgcgcgggg ctgcgtgcgg ggatccccct gggccgccag tatttcttca
      601 gcggaaagcg ttcccccatc cagggtaatt tcaacagtct ccctctttcc tttccggtaa
      661 aatacagccg taatcctgtc tccaggcacc ttttcagaca gggcttccgt cacagactgc
      721 ggaccggaca gggagacatc ccccagcttg accatgacgt cattttcctg gagcccggcc
      781 ttggcggcgg ggctgtccgg catcactttg gtcaccagca ctccggggaa tccactaagc
      841 tccagctgcg caacgagcgc ttggggcacc gtgcccggaa caattcccag tctggccggt
      901 ttggaagagg ggagcggctt cacctgatgc gcgtacggcg tcagaggagg cggctgaagg
      961 ttgtccgtac ttcctgcggg ccggtcaatg gaatatccgg agcccgaacc taccagcaat
     1021 cccaatgtca gaaagataca ggttttcata tccatgtgag ataatataca gcatctgtcc
     1081 atacaatgtt caaaatttgt gcattttttt cgccgcttcc gggacggcat ccttcgccgc
     1141 agaacaccgg aaaacgccag gcatctttcc tgcctgccaa ggaaagccgc aatgccgcag
     1201 cagatcgcac aattccggcg atattcatgc accgcaattt ttaatattcc ttttcaggaa
     1261 gaattttgcg gataacggcg ggagcaatca caggcacgcg ctcatcccct ccctgcaatt
     1321 caaaaagggt gggcgtccgg agcaactccc gcttttcacc gggaatcaca ggagtttcag
     1381 ccttttccga gggaacttcc ccggcatttc cggtcgccac cgtccctacg ggcgccacca
     1441 gagcatgttg aaccaacatg acgcccccca caacggcagc cgcggaaatg ccggaaatac
     1501 aggcaaaacg ccgccacagg gggatgttca gcgctttttc ccgtttttca ttactggcgc
     1561 aattcatggc ttgcgccatt ttacagacgg aaaattccaa catggaagaa gcggaaagcc
     1621 ttttcaaccg ggtttccatc tcatgttcgg attccgcaca catcagggca cagcattgtt
     1681 ccctgaacag gacttttgcc ggagagggcc gaaaacgttt caaaagggat tccatctcat
     1741 gaatttcatc atcggagatt tcatcacgcc ggaaacagct ctccaaaggg aagatttcct
     1801 cacactttcc ggccagccgg gaaatcagtt catcagatac gggaaggggc tgcaggacgg
     1861 cagtaccgcc gtcttcctcc cctgcggcgg atgatgttgt ttcatggttc at
//
LOCUS       DAWEMX010000232         1872 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_18125_length_1872_cov_15.722620, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000232 DAWEMX010000000
VERSION     DAWEMX010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18125_length_1872_cov_15.722620"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          420 a          503 c          570 g          379 t
ORIGIN      
        1 gcagttcact gatgatggaa aggcagaaca tctggatgcc ccacatgccg aagacttcca
       61 cggcctgcgg cagcccgatt ttcaagatgc gcttttgcat gcccatgtcc ggccgcagat
      121 gggaaaagct gaggtacagg ggcggggcat agtgttctcc ccgttcccgg acaagttctt
      181 ccagggattc atttttcctg cccgtgaaga ttttttttct gcgccgcagc atgatgaaca
      241 ggaggatgca catgctgatg ccgtaaccgc agaccgttcc tgccgcaatt cctccgatgc
      301 gccagccgcc cagaggagcg tccgcaaaga cgaagatgac gctgaagatg acattgagga
      361 tgcctacgct catcatgatc cagaagggaa gccgcgtgtc tccggagcct cgcagggcgg
      421 cattgatggc aaagaccacg ccgctgaacg gggccgcaaa tgcggccata taaacgtatt
      481 gcagggcgta ggcccgggcg gtttcattca tggtcagtac gtgcgtgacc aggaaatcgc
      541 tgcatgtgaa gagaagtccg caggagatgg ttcctgccag gagccccagc atggcggcct
      601 ggtgcaggcc atgctgggcc tggggatagt ccctggcgcc cgtcatgcgg gaaacgatgg
      661 ctgtggctcc catcatgata gccccctgga tgacgaagcc gaaccacatg atgaagacga
      721 cggcgcccat gccgtcagag atgttgatgg tgtcctgggc gtccacccct attttagtgg
      781 cgatgaaaag gtcggacgag gtgacgaaaa agctcagcag ctgttccagg aatggccaga
      841 aagcaatgac ggcgatttgc cggggcaggg atagtccggc aagttttccg cccaggcggg
      901 cgcgtgccgc cttgccgcgt atgatgcctt ctcttgcgtc ccccgcactc atcgctgggc
      961 tgtgtaaatg ctggcaattc ccccgttcag agcttccgct gtgatattgc tgtagccgca
     1021 ttcccgcagc agagccttca tggcctgccc gcaggggaag gcttcaatgc tgtccgccag
     1081 gtagtcgtat gcttcccggt ggccggtcat ccaccctgcg atgacgggaa ggatgcggtg
     1141 cagataaaac cggtaagggc ttttcaacag gctttcaggc agggaaaaat ccagaatgag
     1201 cagatggccc cccgggcgca gcatacggcc catttcccgc agggcgtccg gatagctggc
     1261 catgttcctc aggccaaagg cgacagtgac cgcatcatag gaggcggagg ggaagggcag
     1321 gtttatggcg tctgcgcaaa ccagatgatc cagtccccgc ctggccgcta tgtccagcat
     1381 gggctggcag aagtcggacc cggtgaggcg gatttccggc atcgcgttca ggatggccaa
     1441 ggccaggtct cccgttccgg tcgccagatc cagaaggttt tccggtttcc attcggagac
     1501 aagctgaacg acgcgctggc gccatagcag gtccacaccc atgctcagca catggttggt
     1561 agccacatag cgcggggcaa tagcggaaaa agcggctttg acgaattctg gattacgcat
     1621 gcggaaaggg ctggggatag taaaaaagag cactataagt gctcttttta tgaaggaaag
     1681 gggatggggt gtcggacggg gctcgaaccc gcaacaacca gaatcacaat ctggggctct
     1741 accattgaac taccgacacc atttagtgtt gtggtgcggc agacattacc cgtcctcttc
     1801 cattttggca agaagaaaat gagtttaatg agttttttgg acggaatatt ttctcaatta
     1861 tacaatgaga ac
//
LOCUS       DAWEMX010000233         1823 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_18667_length_1823_cov_9.849548, whole genome shotgun sequence.
ACCESSION   DAWEMX010000233 DAWEMX010000000
VERSION     DAWEMX010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18667_length_1823_cov_9.849548"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          456 a          494 c          479 g          394 t
ORIGIN      
        1 ctggtgggtc cagggtcgtt agggacaccc gttggagcca ggattcagga tatataggaa
       61 atctacgccc gcatggtgcg cgtagatgat tcctccaaga ccacaggact gaaaaagaat
      121 gccaactccc gtaaatatat agcctggtgg gtcacggggg aaaaccagaa agcgcgtatc
      181 aatttgatgc cgcatgcttc tgaagaaagt acggatgccc tgacggtgct gcgttccact
      241 tgggatacgc ccggccccga tctggagtct ctcaatcttt cttccgtaat gacggcggga
      301 tccgggaacg acatgtccgc agtcagaaag cttatctctc ttgattctct gctggccaac
      361 aataaaaacg gacgtttgaa ggatttgggt gacgtttacc acgacatctc tctggtttct
      421 tccagtttgc tgacggattc taaattcggc ggtcttaaga aagacctcaa cattatgttt
      481 tcccaggaaa aacttcctga agaatttcgc ggcgatacgg cggacatagg gttgcgtccg
      541 tacacgtccg aggacggcaa cccgtcggac cggaaccggc cgattggctc ctggaaccag
      601 ctttacctgt gggcgaacgt gtgggactcc tccacgaaga gggaaggcca ggatgcctcc
      661 gccacccttc agtggaacgg aagcgctccc agcacgctga ttgcggcgga ctccgcagcc
      721 agcatggaaa tgatggacaa ccgctacacg tacatgcgcc atcccatcct gctgcgcttt
      781 tattcctttg taggacttaa ctacgtcaaa cacggcaaca agcatcccaa ccgtggagga
      841 tggatcgact tccgtattgc ggtcattccc gtgtacgtct ggtggaatcc gtataatgtg
      901 gatatgaagc tgaccggggc caacggaggt gtctggggcg cctacttcgg cgagcaccgc
      961 ttcatgcccc tgctgctttc ccataatgtt aaattcgcat ggaaccaact ggatgagaat
     1021 gcactctcca attatggaga atcctgcctg atcccctatt tgaacgcttc ttataacaac
     1081 cgccaggtgg cggatttcgg cgcctctttc cgaactacgg ccaaacttaa cagtgacggt
     1141 tccaccgtca agggagagcc ttccaccttg aaagcaggcg agatcgtcgt ttttgcccag
     1201 ccggtcgttt ccaatatcgc caacggtaac gaagtttaca aaacaaagcc caacagcggt
     1261 gatttcaaac cggacagatt cccgctcaaa gagggttggt cccaggaacc gggacaagtc
     1321 agctcttatg ccgtcaacct gacggacggc gtccgttatg aacttctgga cggcagcgga
     1381 tataaccagg taggcattgc caaggcttcc gttatcttcg cggaagatac cagccggctc
     1441 aacggaacgc cggacaatca tgttctcgcc gtcaaatacg gagacaaggg tgcctccaaa
     1501 gccctgggaa ccttcgtgat gggggccggc ctgatggatc ccaacaaaat gaacagttcc
     1561 tcttccctga atgggaaaag cacggcggaa atagaaattt tctccaagac catgccggcc
     1621 atcctcaacc tgaactgggg tgcctgggaa aaacctcttg ttgaccaaat taaacttccc
     1681 gcagaaatgt gggaacgcaa cgaagggacc aatgaagaag ctaaaaaagc gggcgaatac
     1741 ggcacgccct ctttggactc cacgtttgtc gcctactatg gagtatcggt aaaatggggc
     1801 aagccccccg tgataggagc cta
//
LOCUS       DAWEMX010000234         1771 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_19277_length_1771_cov_9.626457, whole genome shotgun sequence.
ACCESSION   DAWEMX010000234 DAWEMX010000000
VERSION     DAWEMX010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19277_length_1771_cov_9.626457"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          416 a          525 c          496 g          324 t
ORIGIN      
        1 gggggtaagc ggttaaagaa acggtggggg gttttgggtg ttatacaatt aagcggggaa
       61 aaattaatgc aggcgggtgt caaagtcaag tatccggaac cttttcgggc atgggctccg
      121 catgggggca tccctgattt ttttgctcga accggattcg gagcttcgct atattggctc
      181 ccgttatggc aacaccggat tttcattatc aggatccttt tcccatggaa aaggacgaca
      241 cccaataccg tnnnnnnnnn nccgtctcat cacgtcagac tatgtgagcg taggcgactt
      301 tgaaggacac cccatgctga aagtggaacc ggagggactg cgcctgctgg cagcggaagc
      361 cttccatgac gtggcctttt tcctgcgccc cgcccacctg aagcaggtgg ccgccattct
      421 ggacgatccg gaagccagcg acaatgataa aagcgtggcc ctgacgatgc tccgcaacgc
      481 ggaagtggcg tccgccggca tcctgccctt ctgccaggac acgggaaccg ccaccatcgt
      541 aggcaaaaag ggccagcagg tatggaccgg ctgtgacgac tgcgaacagc tttcccacgg
      601 cgtttacgac gcctataccc agaacaacct gcgctattcc cagacggtgg cgctggatat
      661 gtacacggag aaaaacaccg gcaccaacct tcccgcccaa attgacctgg aagcggtgga
      721 gggcatgaaa tactccttcc tgttcgtcgc caagggcggc ggctccgcca acaaaactta
      781 cctttaccag gaaacgaagg ccctgctcaa cccggccacg ctgaaaaagt tcctggtgga
      841 aaagatgagt actctgggca cggcggcctg ccctccctat cacatcgctt tcgtcattgg
      901 cggaacctct gcggaaaaat gcctgaagac cgtgaagatg gcttccacga agtactacga
      961 caatctgccg actacgggga acgaacacgg ccgtgccttc cgtgacctgg aactggaaaa
     1021 ggaactgcat gaagaagcgt gcaaactggg cctgggcgcc cagttcggcg gcaagtggta
     1081 cgctctggac gtgcgagtta tccggctgcc ccgccacggc gcctcctgcc cggttggcct
     1141 gggagtctcc tgctctgcgg accgcaacgc gaaagccaag atcacgccgg agggcatctt
     1201 tattgaacag ctggaaacca accccggcca gtatattccg gaagccctgc gcatgacctc
     1261 cagcgaagct gtctccattg acctgaaccg cccgatgaag gaagttctga aagaactggg
     1321 caagtacccc gtcaccacgc gcctgagcct gaacggcacc atcattgtgg cgcgcgacat
     1381 cgcccacgcc aagctgaagg aacgcctgga aaagggcgaa gggctgcctc agtatatgaa
     1441 ggatcacccc gtgtactacg ccggcccggc caaaactcct gaaggctacc cctccggttc
     1501 cttcggcccc acgaccgcag gccgcatgga ttcctacgta gacctgttcc aggaaaacgg
     1561 aggctccatg atcatgattg ccaagggcaa ccgcagccag caggtaacgg atgcctgccg
     1621 gaagcacggc ggcttttacc tgggctccat cggcggcccg gccgccattc tggccaagaa
     1681 caacatcaaa aaagtggaac tgctggaata cccggaactg ggcatggaag ccatctggaa
     1741 aattgaagtg gaagatttcc ccgccttcat t
//
LOCUS       DAWEMX010000235         1753 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_19479_length_1753_cov_6.461720, whole genome shotgun sequence.
ACCESSION   DAWEMX010000235 DAWEMX010000000
VERSION     DAWEMX010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19479_length_1753_cov_6.461720"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1340..1418
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          368 a          461 c          445 g          400 t
ORIGIN      
        1 tttcattgcc gcccaggtac aaaaattcat cacagttgcc gatgatggat tcccattgct
       61 tttcaaacag cgccttgagc tgggcaatgt tctgaatgat gatggaaacg ctgatattcc
      121 gggagcgcat ggtggccagc agcttgtcga aatcatccgg caagctcacg ttggcgaact
      181 catccatgag aaaatgcacc ggtacgggca ggctaccgcc gtgtacccgg tcggcagaat
      241 aaaaaagttc ctggaaagtg ctggcataca gcaaacttac caagaaattg aagctggcgt
      301 cgttgtccgg gatgagggca aagagtgcca cttttttctc gcccaggctg gaaaggtcca
      361 gttcatccgt ggcggtaaga gccgccaccg aagaaagatt gaatttttcc aggcgggacg
      421 ccaaggtgat ttggatggat ttcagcgttt tggcagagcc ggaacggtaa tccttgtaat
      481 acttcacagc gatgtgatcc ggggagcgca tttccagccg gtcaaacagt tcgtcaagcg
      541 gcgattggta ggaatcatca tcctcccgta cctccccggc tcggagcatc tccataatca
      601 tggggaaatt cttctcgtcc tccggcgctt cgtaccacag ataaaacacc agcgccatga
      661 ggagcatact ggccgcagta tcccagaaag ggtcgttgga ctggctccct ttcggagtgg
      721 tggctttgaa aaggttcgtc accatcttct gcacatcgtt gtcggatttc agataggcaa
      781 agggattgta gcaatggctt ttctccatgt tcagaaggtc caccaccttg atctcatagc
      841 ccatctgttc caacaggccg ccgcagtcac gaagcagttc ccccttacag tccaaaataa
      901 aaagggacgt attgcattgg cagatgttca ctttggcgta gaaccgactt tttcccgcac
      961 cactgccgcc caccaccaaa gtgttgaggt tgcggcggtg cttccggcca tccagcccca
     1021 tgcggacgtg ctgggtaaaa atgcggtttt tgaacggatc cttgtcccgg tatttcttat
     1081 tcacggcccg cggatcgccc catttggcag agccgtgttc ttcaccccgt ctgtagttgc
     1141 gccgggaaga aagataaatg cctatgccca gcccataggc ggcaataaaa atgaggacag
     1201 cttttacact gtcctcgcac cacacgattt ggaatggatg gttcattgcc gcaggcaggg
     1261 cgtttacaat ttccgaaagg cccccggaaa ggggcggcgc aatcaaaagg gcaaaccaga
     1321 ctaccgggat cagccccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gcccgccttg ttatcgggct
     1441 tcatcccggc gcttcgcttt cctgtctttt tgggatgggg cgtcaatggt ttttaagagc
     1501 acatcttcta ccgggccagg gtcgcgctgt tcctccagca gatcgttgcg cagctcgttg
     1561 agggcgcgga tcataatccc gtgctcgtag ctgtccaggg tgaggacaac cttgtcctgt
     1621 cgttccattt ttcaaaacct cctttaccgt tcctgttcct tctgcctgct ggcgcgggta
     1681 aggcccatgg cccgtgccat gcgcccgttc atctccttga acgtatcgcg catatcggtg
     1741 agggctgccg gaa
//
LOCUS       DAWEMX010000236         1623 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_21248_length_1623_cov_9.709184, whole genome shotgun sequence.
ACCESSION   DAWEMX010000236 DAWEMX010000000
VERSION     DAWEMX010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21248_length_1623_cov_9.709184"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          466 c          373 g          367 t
ORIGIN      
        1 cacctccttc atctgcggca ttccacacat aatccgtcgt atccgcctgg accatcatga
       61 ttgagggaga gacaactcca tcaatggtta ctgtttgcag agaacttccg tcaatatctc
      121 ccatgaccac tccggaagca ttattgtaca caccttcatt tttccacggt gaagaagccg
      181 gagcagttac agaccatatc ccgtcgccgc ctttccattc aagcaaagcc acctgctcca
      241 caaccagggt aagattattg ttttccgtgc tccatgaacc cagaagattg tcgcccagaa
      301 cgaaagagct ggcagggact ccctctacgg aacccgcatg aaccagactg taggaaccct
      361 ccgtccaggc tacgccttcc atcgtttcct gccccagaag caattcactg cccacggtca
      421 gcgttcctgt tacattaagc aaatatgatc cggaagccgg aagagcggaa aaattcagga
      481 gagacgtgtt tcccagcgtc aaattccccg taatggtccc tgaagaaccc agtgtcaaaa
      541 cgccggcatt cgcaatgcca agcgtccctc cgttcatgtt caggacagcg gcattggaaa
      601 ggctgacact ggcaaaccgc gtggcagcat tcacgttcat gccggaagaa ccgctcacgg
      661 ccagggcatt gttataacca gcatcgtgct gggccgttat attgccgccg tttaagctga
      721 ccgatccgga ccccaaagcc gcggcattgt tcagttgaac ggtcgcccca tgggaaatga
      781 cccaatcagc cgtcatggaa gactgggcag ccgtattcag gataagggtt cccgcaaaac
      841 cgtccgtccc caccgtcatc gtcctggctc ccgttccagc cgttcccaga ccaccgtaaa
      901 tattcatggt cttgccggag gcaatgttaa acagggcgct ggagttgaaa acaactccgt
      961 tccatacctg gctggcaact cccagagaaa aatccccgcc tatgtcaaac agcgtatacc
     1021 cttctcctcc atcacgtaaa ttaacggtgc ggttggagcc gttgcttttg gaaacgctga
     1081 atccatcgga attatccgta acggttattc ccccaagtga aagcgtattg aaagaagcct
     1141 ggagagcctt gacctgcccc gtcatgctac ccgcatcaaa acgcatgctg tttgtcgccg
     1201 ttccggccca tgttgccgac cagttgctgc tgccgtaatc cggagtccag ttagtattca
     1261 aatgaacatc caccgtcccc cccagccatg tataggtttc cgcaacggca tgcggtgagg
     1321 aaacagcagc catgcaggca agcagggagg acaaaagact taaagggagg tgcaacttca
     1381 tagtttttaa aagggcgtac gtgaaaattc ctcccgaaac tgacaggagg ctttttacca
     1441 tcattagcgg attaaaaatc ctcctacaag cttaaacatt gattccgtag gtgttgcgca
     1501 tcatccagaa gagtatttcc atgctttatc tggtctggaa tcagggacat ccatcgttct
     1561 atccttatca gtataagaaa acaggcgggg caactctggt atattggcac ggttcacatt
     1621 aca
//
LOCUS       DAWEMX010000237         1588 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_21754_length_1588_cov_12.013699, whole genome shotgun
            sequence.
ACCESSION   DAWEMX010000237 DAWEMX010000000
VERSION     DAWEMX010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21754_length_1588_cov_12.013699"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          391 a          466 c          449 g          282 t
ORIGIN      
        1 tcccgccttt cacgcgggca acacgggttc gagtcccgta cgcgatgcca gctttaccgc
       61 cgcggcagtt acgataattc cgcttttccc cttttcaggg aagcatattg gaaaaacgta
      121 atggctgcgg ttttcttttg gcccggtttt ccggattcag gaaagcgcgg cagaggcgcc
      181 ggaagccgaa tccccccctg cttttaaatg ccggaacatg tcttacatcc cccggatttg
      241 gctttcagaa agccccgcca tcttttaaaa ttctttacac gcaaacgcca tggattacac
      301 acctctcatt gaaaaacgcc gccagcgcct ggaagaattg gaaacagtca ttgccgaacc
      361 ggacttcttc aatgaccaga aaaaggcctc ggaaatcatg agagaacacc gccgcctgaa
      421 ggaactcatg gaaacatggg attccctgaa cgccacggaa caacagctgg cggacaacca
      481 ggaactggcc aagacggacg atccggaact ggcggaactg gccgccctgg agattccaga
      541 actggaagcc gccctggaaa aactgcggag cgacgtgcag tacagcctgc tgccccgcga
      601 cacgacggaa gaccgggacg ccatcattga aatccgcgcc ggaacgggcg gggatgaagc
      661 ctccctgttt gccggagacc tgctgcggat gtaccagcgt tttgcggaag aacgcggctg
      721 gcgcttcgag catctggaaa gcagcccgtc ggacgtgggc ggattcaagg aagtcgtctg
      781 ccgcattgcc ggggaagaag tgttccgctt cctgaaatat gaaggcggcg tgcaccgtgt
      841 gcagcgcgtt cccgctacgg aaacgcaggg gcgcatccat acttccaccg ccactgtggc
      901 cgtcatgccg gaagcggagg aagtggacat agaaatccgc ccggaagatc tccgcatcga
      961 agtatgccgt tccggcggag ccggaggcca gcatgtgaac aaaacggaat ccgccgttca
     1021 gatatggcac atacccacgg gcgtttatgt ccggtgcgag gaagaacgca gccagatgaa
     1081 aaaccgagaa aaaggcatga aaatcctgcg cgccaagctc tttgaagcca aaaaacggga
     1141 agaggcggaa aaatactccg ccgcgcgccg caatctcatc ggctccggcg gccgtgaaga
     1201 aaagatacgc acatacaact tcccgcagaa ccgtctgaca gaccaccgca tcggctacac
     1261 ttcccacaat ctggacggta tcctgatggg gcagctggaa gacctgatta tggccctcca
     1321 gcatgcggaa atgcaggaac gcctggcgga ggccggaatg tcctaacttc ctttttcctt
     1381 tcatgaaaac tttactggaa gtccttcagg ccggcacgga ttatctggcc cgccaggggt
     1441 gcgacgaagc acgcgccacc atgcagcatt tgctggccca tgtcctgcac tgcaaccgca
     1501 cggctcttta ctcccagttt gacaggcccg tggaagaagc ggagctggca cccctgcgcg
     1561 aactgctgaa acgcagggca gcgggaga
//
LOCUS       DAWEMX010000238         1582 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_21864_length_1582_cov_6.335298, whole genome shotgun sequence.
ACCESSION   DAWEMX010000238 DAWEMX010000000
VERSION     DAWEMX010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21864_length_1582_cov_6.335298"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    667..676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1030..1039
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          401 a          430 c          407 g          324 t
ORIGIN      
        1 gccccctgtc caggtcatta ccggagaggt aagcagcgtc aggcgcggtt cccccatgtg
       61 gaccttcggt tccggcgtaa tggcaacgtc ctgcgtataa ggaacggaga actggaaggt
      121 aagaacatgg attcccgcct tgttgggaac ctgggccacc agccagtgag ttatgccggc
      181 aatgggaaac atggagggag cgtccgcctg gagatgccgc ccctccacca gggaaaaatc
      241 acatttcaga tcatccacgg atgtgacgac gtcctgcacc gctttcgtaa aagggaggct
      301 gcgtatgggt tcctgcctgg cggcattggc ctgttcatca ggcggttcgt actggaacgg
      361 cacgcctacg gaaatggttt tccccctgat tcccctgggg atatccagcg cgcaggtgta
      421 aatgaggaag gcctggtgct gcttcaggga aacagtaacg tccagcgcgt tcagccggat
      481 tccgtccgcc tgcgtcaaat ccatgggaac gggcaaaccg ggaaccggag cgatgcattg
      541 aaaggatatc tcctgctggc gggggcggtc cgctttttcc gcggcggtct gttccttctg
      601 ctggggttcg gcggccatgc ccggcacgca gcatgcaatg ccggccagcc agatcagggt
      661 agccaannnn nnnnnngtta gattaacgcg tggcagcccg ttcatcaagt ccatttaggg
      721 gagggggaaa ggtcccttcg tacccgccct tttccctgaa caaacaaggg cggccctcct
      781 ttgaaccagc gacgcccgca gcaggaaaac attcgttcca aactccggaa ttggtcgctt
      841 catgcccttg gaagcgggtt ccggggttcc gcctggacgt ctctctgccg gagaagcgga
      901 aagatggccc atggcacatg acaggtaaaa accaactgag gggcatcgga gggccttttc
      961 cgccacaaac cagcataatt aactcattaa caacaatcgg catccattgt tgcatatttg
     1021 cagaaagacn nnnnnnnnng tgttcttccg ctttccaggc gcctttaacg tactcttatt
     1081 accagggttt ttcatgaaaa gcccgtttcc gggggagagc caaaacgccc agccgccccc
     1141 ccttcgccaa acaaaaaaga agcatgcgga agacagaaaa aatcacgcaa gacaggagaa
     1201 ttttttctag aaaacagaac aaataataca atatatagtg tatataaaaa aatactgtac
     1261 aatatttcct tttcatcagg attatttttc agtagataat gcacacgcta cgcgccgccg
     1321 ccccatgacg aagcgcaaaa gcctacggga ttattcccaa acctttctac ttcgcaatcc
     1381 aatgatcaca gacaactcgc aaaccatact caaactgcgt acaggccagg aaatcatcct
     1441 cccccagagg aaagacctgc tgggcggttt gaaattcacc agaagatttt cacacaatga
     1501 agtccacccc tatgatgaag tggactggac acgccgcgac gtccgcatta tggattggaa
     1561 aaccggcaag accatttacg ag
//
LOCUS       DAWEMX010000239         1578 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_21917_length_1578_cov_9.097177, whole genome shotgun sequence.
ACCESSION   DAWEMX010000239 DAWEMX010000000
VERSION     DAWEMX010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21917_length_1578_cov_9.097177"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1268..1277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          394 a          375 c          465 g          334 t
ORIGIN      
        1 aagcagttgg attgtaagaa ggtggcttcc cgagcctgga aaatgttcct ggcggaagtg
       61 tctgaagaag gccttgccct gatggacgac cagacggcac gtgaggcggc ccgccgcgct
      121 ttccgctgtg cggaattctt catgatggag gaagcgcgcc gcaagcatgc ccagaaagca
      181 gctctccagc agcaggagaa aacttcttct ccggaagagg aacgggattc cgaagaggat
      241 tccggcgaag aataattcct gtctgtgttg atgtttttgc cctgcggcgg atgccgcagg
      301 gcgtttttgt atcctccggg cttattttcc ggagtttgaa tggccgtgtt ttatccggtg
      361 tattgggaag cagcatgcgt caccctttgc cacctggggg caaaaagcag ccttttctcc
      421 aaccagggca ctttttgaga atgggagagc gttggagacg cctggatgaa agcggacttt
      481 atttcgtgcc tttccccgtt cttgttcctt ctaaaccttt ttgcctctcc atttcccgct
      541 cttgaggtac caccatgcca ggatgatcag ggtaacgccg taaacgtgtt cggaaaacca
      601 gcagagagcc acatccggct tgaacctgat gatgccgtaa taaatgacgg ccgtgtaaac
      661 ggccagggaa atcatttcca tgatgaatcc cgccttggta ttgcctgtgc cggatacggc
      721 gttaaacagg atgaatgacg gagccatgat cagtgtggaa gaagccatca catacacgga
      781 attgacgctg gcggcaatca gggacggact atccgtgaag gcacggagga cgaactccgg
      841 aaacaggcac agtatgatga gcagggggag caggatggcg tatgtcagtt tgagagtcat
      901 gccgcaggtt ttgaacaggc agcgctgttc tcccgctcca atctggttgc tgatgagagc
      961 tcctgccgtg gtggcaaaag cgctgatcac catgatcagc agggcggaaa tattgcggac
     1021 gatgttggta acggccagcg ggcgttcccc caaatgttca atcgccagga aaaaaataaa
     1081 ccagatggag acggaaataa aagattgcac catggtccat ccggaaatgg acagcattcc
     1141 cttcagcaga tgggggcgga gtgtgaatgc ccggcggaag ccgtacttcc gtttgtccgc
     1201 cgtcatgctt gtgtagatga tgaaaaagag aagggagact gcttccgata cgctggaggc
     1261 aatggccnnn nnnnnnnccg cggcgggaaa gccgcctttt ccgaaaatga ggcagtaatt
     1321 taacgcgaca ttgctcagga gcatgacaat agaattgacc gtcagaatct tcgtctgggt
     1381 aatccccacg taataggctc ggaacatgac gcagaggaaa gagaaaaaga taccgtaggt
     1441 gcgccagtgc aggtaatccg ccgtggcccc gtacacctgt ggggattcaa tgatgtcccg
     1501 caggatgatg ggggaaaaat attgggatgc ggcgaaaagg gctaccgcca ggaccagcat
     1561 gaaataacag ccctgaac
//
LOCUS       DAWEMX010000240         1556 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            NODE_22287_length_1556_cov_5.190540, whole genome shotgun sequence.
ACCESSION   DAWEMX010000240 DAWEMX010000000
VERSION     DAWEMX010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22287_length_1556_cov_5.190540"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          425 a          464 c          296 g          371 t
ORIGIN      
        1 cacgcgcaaa aatccattaa aaattgccta cggcaatggg atttttgcct gctgaaccgt
       61 tacgtcctat ctacaaaggc cccgctgcca gtattttggc ggcggggtct ttcgcataga
      121 tttccttatc tccttatcag aatgacacac aatccacaat aggacggttg atgatcttct
      181 ctacgcccgc caataagtcc ttgtcaaaca tgggagccgc caacttcaga tgctgataac
      241 ccacatgaat gagataaact tcctggccct gcttcaagtc agatgtaggc gttggccgtc
      301 cagtctggct gtcaaatgtc ataatcagat cagggaatgt gcctctgcgc tgtccatctg
      361 gtccatccac cgtcatgtac tcattccaga aagacatctc atatccacca atcttaacga
      421 cacctacatc aaaaccgcct tcactgcgca gctgatactc ctctaccgga ccgtgagcca
      481 atatctcacc gcctaaaaat tcacaaacct cctgcacacc attttctacc gacttttcta
      541 atccctgtga ataacggcgt cccgtctcaa tagccagtga caagcccccc acagcgctgt
      601 ttttttgcag gaacgaagcc ttcaccgggt tccggattac agccacaaga cctccagcct
      661 ccacagcagc cgcccgcacc agtttagaac aatgatcaat agaacctgta actgtacatt
      721 ccacgtgtcg gcccaattcc tttcgtccgc ccacacaggt catggtagta atataattgg
      781 gatcacgatg aagattcagg cttcccatca ctcccgtagg atgagcacga ccattgcagg
      841 gagcgtctat cagagggatt cccagcatgg atgcctctag ccagccattg aacgtagaac
      901 cgcctccatt ctcattagta acgatacctc ctggacgaat acccgtgctt tgagtaaaga
      961 gctccacgca gcgcatcata tctgccgggg agacaaactt ctcctgtgcg gcaggagccc
     1021 ctaccataga ggctgtgaca accacggttt ccggatcaat ctgatccaac ggggttaatt
     1081 ccagctggct gaaccgcaca gccagatctc cgaactcagc tccggtcttt gccgaaccgc
     1141 ctccaccgcc gccaagaatg gagccaccaa gagccgccca acggacatgt tcttctgtca
     1201 atttcattcc aatatgttcc ataactttct tcctttctat attctgcatc aaaatgcttc
     1261 caccttaatt cttcttattt gccaccagat taaataccac atgcagcaca accgtagcta
     1321 ccataccatt aatagcctga atgcccggag agacaatagc aactgctaca ccacatcccc
     1381 aagccagaac ggaaggccac ttgataagtg gaaggttttc gtaagaaatg tcattataat
     1441 tataattatg ttttactcca aacaaaggca gtaccaaata atcagccacc ataactgcag
     1501 ccattggcgg aattacaata ccaagccatg tcatatacgg cacaaagtag tccacc
//
LOCUS       DAWEMX010000241         1402 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_6625_length_1402_cov_18.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000241 DAWEMX010000000
VERSION     DAWEMX010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6625_length_1402_cov_18.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          287 a          387 c          398 g          330 t
ORIGIN      
        1 gttatttttt acttatatat ttttactgca attacaaatg acggcaatct tgatgccttt
       61 catctggacg ttggtttcca gggcttccgc gatttcatcc agcttgaagc ggtgggagat
      121 cagcttggtc agcggcaggc cgatgccgta ggctcgcttc agaaagtcga atgtggtcgg
      181 gtagtcctgc ggggagtaca cccaggagcc tacggcggtt acctccttgt tgcagaggtc
      241 gtaatggtgg ttgataacgc tttcccctcc atccatgaaa aagcccactt cgcacaggcc
      301 gccgccgcgc ttcacgaact tccaggcgtt ggccccggcc ttgcctacgc ccgtgcactg
      361 aaagacgaag tccgccaggc ggccgccgtt gtcctttttc acggcatcca gcagttcgtt
      421 cagatccgcg taccgggtga agttatacgt gcgggaggct cccatttccc tggccagttc
      481 caggcgggag tcattgccgt ccacagcgat gatggtttca atgcccagcg tgcgcagcgt
      541 ggcgatttga agaaggccga tggggccgca gccctgcacg agaacctcgg aattgaactt
      601 gaggagcccg gtggacttgg cgcgttccag ggaatggacc accactgcgg cgggttccac
      661 cagaatgcgc tggtccagcg tcatgccgga aacattgaaa aatgtggagc ccttgcggat
      721 gacgaggtac tccccgaagt agccgttcag atgcacgtcg tcgtcaggca tcaggccata
      781 cacgcccaca ttttcgcaca ggttggtgcg ggccggagta ttcaggcagg cgtcacaggt
      841 gccgcaggga atgatgcagg tgacgatctt gtcccccagc gctacgggat ttccggcggt
      901 gtctttagtg atattcctgc ccatggcgac gatttccccg gtgccttcat ggcccaggac
      961 cacggggcag aggccgaagg ggtcccggtt gtattcgtgg ccgtccgttc cgcaaacgcc
     1021 gcaggcttcc accttgatca gcatttcatc gtctccgatg gcgggaatgg gatattcacg
     1081 gatttcaaat gtctttggtg ctgtcaggac agcggtgcgt gcggtttgtt gcatgatgcc
     1141 cttacactat cctttgccgc ccggattctt caagaacaaa tagtgatgaa aggaactccg
     1201 cctgttggaa ccggggggaa gttccttccc ggtccttcct gcaatgcacc ctggcgccgg
     1261 atgtccatga ttcccggaaa catggagagt ccggtcctcc tgctgtacgg ttttcctgag
     1321 aaggaaaaaa atgggaccta catacttgac taaccatcca acataacacc tctttctttt
     1381 cagaaaattt ggtaaataaa aa
//
LOCUS       DAWEMX010000242         1378 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_51104_length_1378_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000242 DAWEMX010000000
VERSION     DAWEMX010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51104_length_1378_cov_14.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          280 a          414 c          354 g          330 t
ORIGIN      
        1 ccggtggtgc tggatctgga cccgcgggat tttgccgtca tccggcagtt cgccccgcat
       61 tttgaccagg ccggcatggc cgttacgccc tttggtcaga atacaatcag aatagaatcc
      121 atccccgcac tgctggaact ggaaaacgca cgcgcttttc tcctggagct ggtggaccgt
      181 ctcacccagt ccgaattcag ccgaaatgcc aaacgcatgg cttatgaaac cttcattggg
      241 gaatttgcca gaaaatccgc ctggagggaa cgcatttccc ctcaccgggc ccctgccatc
      301 ctaaaggatt tgcttgcctg tgaagtgccg tactgcaccc cgggtggcaa acccacgctg
      361 gtgaattatt ccgttccgga aattaaacgt aaattcggcc tacaggcata aggataaggg
      421 aaccttgttt tccggaaaac aggccgaaac gctgcaagat tttacggcaa acggaaaata
      481 tgcagccaaa gacggattga cgcccgcttc cgcagtatat ttattttccc ttaccgatac
      541 tcagttttat ttcagccgca gcaaggggca gaaactctcc ctggcaacag ggagcatttt
      601 accctgtccg gctacggaca ccttttccgt cttgtccgcc agaactctgg cattcggacg
      661 catcctcccg catacaacag ggagcttgct cttggcaagg aagtttcccg cgtttatact
      721 gcagccttcc agataaattc ccagccatgc cagactctcc ccccagcacc tcccccgctc
      781 cgcgcattgc tgaaactgcc gccgggaaaa ttctggcggg cctcctggcc cttttttaca
      841 tcttcctggc aacgacggcg gcaggcgggg aatggcacac ccttttcctt cccgcctgct
      901 ttctggcggc agccctcctg ctgttctgct tctgtattct ccggggatac aagattccca
      961 gccccggact gccgggctgg ctggccctgg gcctgggagg cggctatttc cttgtccggg
     1021 catggttttc cccctggttt tattatgaga gcgtagcgga tctgggcctt atcgccacgg
     1081 ccatcgtcat gttcgcggcg ggaacgtatg ccggagccgg aaacggggaa aagagtattc
     1141 ttcccgtact ggctgcggca ttggggctgc tgaatgccct gctgtggggg tatcagaaca
     1201 taacgggaac ggaagcctcc tggttcagac cggattattc cctgttcggc acggaaatcc
     1261 gcaatatcgg attgttcgga tacaaaaatt tttccgccca tttcctgtcc gtcaccggtt
     1321 ttttcctttg cgcatacagc atggcttccg ccagaaaatg gggcatccgc ctgttcac
//
LOCUS       DAWEMX010000243         1343 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_81791_length_1343_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000243 DAWEMX010000000
VERSION     DAWEMX010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81791_length_1343_cov_14.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          321 a          401 c          338 g          283 t
ORIGIN      
        1 ctccggcccc ccctggcgcc aatcgggtca tgactcgaac accacccggg cggataccgt
       61 cttattctgc atgcacggca ttattccacg gatattcctg gcgctgggaa caggtttggt
      121 tttttcctgt tccagttccc ggacaatgcc cgtttcctct ttgattaccc atccccttca
      181 cgcggactcc aggcagagct gttttggagg cgtctggtat aatccctcgg aacaggccgt
      241 ctggaattct ccaaggctga aagtatatgt tgcccccgtc aatattgatt acatcaaagc
      301 cagattcccc aaggaatccc cggcgctggc ggcacaattc agggccgggc tgcagaaaga
      361 catccgcaac gtgctggaaa acaaatccag acagacagac gggagaatga aatggcagtt
      421 ggttgaccgc cccgttccgg gatccgtaac tctcagcctc gccatagtca agctgaagcc
      481 tacggacgtg ggcggcaaca tcatgtccga tcttgtttcc ctggtctccc cacttccggg
      541 aacttccctg atcctgggac gcttcatgag cggggacgta ggcattgagg gacgcctgtc
      601 aaatacgaat acggacgccg gcatcatgga attcaaagct tacaacacgg accccatcac
      661 cctgttttcc gtaaaggaat ttgaacgttt tgccttcgac caacggaatt tgcggctttt
      721 ttccagctgc attgcagata ttttcaaggc tggtcccgcc agcagtattc ccaaaacagg
      781 ggaatttgac ctggacccgt tttaaacggc ctttccggac gattctttcc cgtctttctc
      841 ttcagggtca tagaaagcgc attcctgaac cgtctgcctg gtcttgaagg catatacgaa
      901 gaaagcggcg cccaccagca cgaggaaaac ggagaggaac tgcccgcggg tcaatcccat
      961 ggagaagggg gaatccggct cccggaactc ttcgcaaata attctgcccg cagcataaag
     1021 aacggcaaaa ataccgcaga acaagccgtt ccacgcacgg ggaaacctta cccgtacaaa
     1081 ccacagcacg gcaaaaagaa acacaccttc cgcaaacgct tcatacagct gggaggggta
     1141 gcgtgcatga tcctgcatca tccgccccac gatttccctg atggcgggcg tatccctcac
     1201 ccggtctgtc acccaggcgg cagtcattcg ttccggcacg gctatgccgg aaagggacag
     1261 tttgtccagc aatccgggcg tttcataaat acgggaggcc acccgcacga acaaatccgg
     1321 atcctgggaa agctccgccg gaa
//
LOCUS       DAWEMX010000244         1235 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_66278_length_1235_cov_13.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000244 DAWEMX010000000
VERSION     DAWEMX010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66278_length_1235_cov_13.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          220 a          334 c          366 g          315 t
ORIGIN      
        1 ccggggttct ccctggcttt ttccagcgcg tgccgggcag cattgaaaat gacgcgtgcc
       61 atcacctcgc tggtggggtc ttcccggtcg aagggaatga tgcgctcccc gtatgcggcc
      121 acgaaggcgg cgtaattggg gtcctccgta ttcacgcaca gggaatggtc aaactgctgg
      181 aggaaatccc ccatcatttg cttgacggcc ttgaaatcca acaccatgtc attggcatcc
      241 agcgtttccg cccggaacac catttccacg gagcgcgtat ggccatgcgg gaatttgcaa
      301 ttgccgggat gtttggacag gagatgcccg ctttccagtt cgatggattt gcaaattcgg
      361 tagaccatga agacaggata acgtgggcgc gggtgcgggg caatcccaat cagagtctgt
      421 ctggttccta tcctgccgcg tgcggaggga cggggcttcg ctgctccgga tgctccgtgg
      481 gcggctttcc cttgcgcacg acccctgttt ctggcaaact ttaaaaaacc atatttttac
      541 cctatggctt atctttcatg gtctttcctg aaaatggggg agaaggcgga gcgcaggaca
      601 gcctttcttt tcctgctcat ggcaggcgtg cctgtcctta ttgtcgcttt cttcgctttc
      661 gtagcgggaa tggccgctat ttatatgcag gccccgtcct ggcgctgatg cttcttccct
      721 tctatttgcc ctgcgctctg cggaaggaaa agacgcggaa cgcctgctcc cgtaatatcc
      781 tgtttggcag ggaaccgctg acggtttctt tcaggatgat agtgaagaac tgggtatatg
      841 tcatttacgc cgtgtttccg gcgttgccgt tccttttccc cggagggaac atgcccctgg
      901 acgggcaggg gaacgggcgg aacctgacag cattttttct gatgtggata tccctgtact
      961 ccgtctttgc ggtactggcg gtctttcttg gaaaggcgcc gtatttgaag ccgggcgggg
     1021 tgatgctggc tgccggcgcc aatctggcgc tgatgctggt ttattacctg tttgcgtgaa
     1081 ggggcgcgcg aggcattccg gaagtttcct cccggatatt cgttccgtct gtactgggag
     1141 ggctgaaccc ggggcataaa aaagccggag ccccgaaagg ctccggcagt gaatcatctt
     1201 tttcccggtt caggcttaat gcccgcaacc ggcgc
//
LOCUS       DAWEMX010000245         1145 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_49043_length_1145_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000245 DAWEMX010000000
VERSION     DAWEMX010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49043_length_1145_cov_12.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          214 a          374 c          293 g          264 t
ORIGIN      
        1 acattgccgc cctggttgcc atctccgtcg ccacgacaat ttatactgcg atcggaggat
       61 tgaaagccgt cgtctggacg gacgtcctcc aggccgtcgt tctgggagtc tccatgcttt
      121 ccgccctgtg ggtactgttc tcccatatcc ccggagggtg gagctccatc tctgccgcca
      181 tgaatggcgg agacgattgg aaattcttct cctggggaac cggagaaggc ctggacttcc
      241 tccaacaagg cgcccgcgtg ctgggccagg aatacacggt gtgggcagcc ttcctgggag
      301 caaccttcat caccatggct acgcatggca cggaccagga catggtgcag cgcatgctgg
      361 cggcaaaaaa cagcaaggcg ggaacgcgtg cagtcatcgt atccggcttg ctggacttcc
      421 ccatcgttat cattttcctc ttcacgggca ttcttctcta tgtcttctac caatacaacc
      481 cggcaaacct ccccgccgac acgccgcagc tgcatgtgtt tccctacttc atcatccatg
      541 agcttcccaa cggcatccgc gggctgctga tcgccggact tctggcgacg gccatgggct
      601 ccctctccac ggctctaaac tccctggcga ctaccgccac caaggactgg taccagggga
      661 tattcaaacc ggaagccacg gaacggcagc tgctccggtg cgtgcgctgg ggaacggccg
      721 gtttctccct gctgctcatc ctcgtgggtt ccatcacagc gtggtatgtg gtgcatcacc
      781 cggaagtccg catcatccaa atagccttgg gcattttcgg ctacacctat ggctccctgc
      841 ttggcatttt cctgttggga atgctgaccc gcaccagagg cagcgactca ggaaacatca
      901 ttgccatggc ggcaggattc tttgtcatcg ctttactgac ggaacttatc cctctgccct
      961 ccggctggca acaatatgtt ccggaaatag catttccgtg gcgcgttacc atcggaactc
     1021 tggttacttt taccgtcggc ttctgtttca gaaagcgccg ccttcccatg cgctgaacgg
     1081 tgaacgccgt cccccccccc ggccacattc atgttccgca ttccccttca aacctcccgt
     1141 caatg
//
LOCUS       DAWEMX010000246         1132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_16291_length_1132_cov_16.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000246 DAWEMX010000000
VERSION     DAWEMX010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16291_length_1132_cov_16.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          208 a          307 c          352 g          265 t
ORIGIN      
        1 agaatgaatt cggtattcac ctgggggatc ccgtggtgcc ggacgtgaaa ttctccccgt
       61 tggaaaatcc gttccgggtc atggggaaag cctttgacaa ccgcgccggt ctttctgtga
      121 tgatagaggc attcaagaaa ttatgccggg aagggcatcc caacaccctg attgccgccg
      181 cgacggttca ggaggaggtg ggcacgcggg gcgccaggac ggccggcgtc gccatgcaac
      241 cggattgcgt gattgttctg gaagggccgc cggcagacga cacccccggg tttgccgtga
      301 cggattccca aggggccttg ggcggaggcg tgcagatcag actgtttgac cccacggcca
      361 tcaccaatcc ccggctggcc gccctggccg aaaaaacggc tttggatgcg ggcatcccat
      421 tccagttgac ggtgcgccgt tccggcggaa cggatgcggc agccctgcat ctttccggaa
      481 agggtgttcc ctctattgtg ctgggaatcc ccacccgata cattcatgcc cataacggcg
      541 tgctggattt gcgggactac cgcgccgcgg tggaattgac ggtggctctg gcccgttccc
      601 tggaccagga ggccgtggaa gcccttaccc attacctgcc ctgaaagaaa cgctcatgaa
      661 tccgaccgtt tacgtacatg acctggaccc tgtcatctgg cagataacgg actcaattgc
      721 cctgcgctgg tatggcctgg cctatttaat gggcttcatc ggcgggtatt atctgctttc
      781 ctggctttcc cgccggaagc tgtatcctgt cccccaggac aaaatggcgg attttgttac
      841 ttatgtggct atttttggcg ttttgatcgg cggaaggctg ggatatgtgc tcttttacca
      901 gattcccaat cacggctggt cccagtttct ggcggatccg ctcatggtcc tgagggtgtg
      961 ggaagggggc atggccagcc acgggggaat gattggagtg gggctgtata cattttatta
     1021 tgcgtggaag caccgcgtca agtgggtggc gcttctggac ggcctggcca ttgtagctcc
     1081 cgttgggctg tttttcgggc gcatggccaa ttttatcaac ggagaactgt at
//
LOCUS       DAWEMX010000247         1074 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate gSpreadC_ID_3553
            k141_84711_length_1074_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWEMX010000247 DAWEMX010000000
VERSION     DAWEMX010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997065
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (human gut metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Pseudomonadati; Verrucomicrobiota; Verrucomicrobiia;
            Verrucomicrobiales; Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84711_length_1074_cov_10.0000"
                     /isolate="gSpreadC_ID_3553"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          227 a          317 c          292 g          238 t
ORIGIN      
        1 aaaaagccct gcatgaatat tgcagggctt tttatttcac catttaaggc tgacgggatc
       61 cgtatgataa aggattcccg cctcgtcccg gacaaggaga gtcaactgga tgcgcgccgg
      121 caaccgcagc atctgaaggc cgttgaacgc aaaaatctgc ccctggcaca acagggggcg
      181 tgaaccgaac agttcatccc cttcaatcac ctgggtgccg tcttcatggg aacggacggt
      241 ccactttttg ccgatgatgc gcacggcgcc gtctcccgca ttgcggatgc ttacgttgta
      301 aggcaccggg atgcggccat catcccgaat catccgctcc agtttttcat tccgcaagta
      361 caggctgcgg ggagtaagac gcacttccag actggtcaaa aggggaagaa ccatagatgt
      421 atgcctgccg gagagaggaa ttatgattcc acaggggcca gagccgcctt gagcaactcc
      481 agcgtcgttt tgcggatagc ttccacgccg gccttggcgg gtttggcgtc cggagccagc
      541 gtcatctgct cctgcaagac cgtcagaagg tccatgattt tggcaatgtc ccggcgttcc
      601 ttcatttccg gctccacccc gtccagaata taatgaaaat attccgggac atccggctgg
      661 aaaatgactt ttgccttgtc ctccgcaaat ttgttatcca gagccgcggt agcggcgtcc
      721 agggcgcgta tccatccccc caggccgatc aggttcgcca tatcggaatc cctcagggag
      781 gcgagttcct gttccacatc ggattgaata agggccaggt tgtttttgaa actgctcaaa
      841 tccccgtctt ccgcattttt cagcaggctg gcggcgtggc ggttcaccct ctctccggcg
      901 ccaatggcct ttgcatagct ggaaagatcc agagcaatgg gtttcacctc attcatcttg
      961 ccgcactgca cgattacgaa gccgtccgcc atcatgccgc ccaactggag agccagggaa
     1021 cagcggtcca tgggaggccg ctccgggcgt gcgcgcaagg ccaccttttc cggt
//
