LOCUS       DAWENO010000001        52331 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_294_length_52331_cov_11.149935, whole genome shotgun sequence.
ACCESSION   DAWENO010000001 DAWENO010000000
VERSION     DAWENO010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 52331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 52331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..52331
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_52331_cov_11.149935"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13416..13425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25769..25778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    35633..35642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    46332..46341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    49121..49136
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    51608..51617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12952 a        12941 c        14536 g        11836 t
ORIGIN      
        1 atagtatgta gtgtcagagc catggctctg gcttgtgatt gaatgaaagt gaggcacaga
       61 atatgtgtgg aattgttgga tatgtgggaa agcggaactg cacagacgtt ttgataaacg
      121 gactgagcaa gctggaatac cgtggctatg actccgccgg gatcgccgtg ttccaggatg
      181 gaaagatcgc tgtagccaag tccaagggca gactgaaaga cctggaggac aaaatggagc
      241 gtgagggcag accggagggc aatgtgggca tcggtcacac acgctgggcg acccacggcg
      301 agccgtctga cgtgaactct catccccaca gcggcggcag agtgaccatc gtacataacg
      361 gcatcatcga gaactataag gaactgaaag atcacctggc atccaagggc gtgacctttg
      421 ccagcgatac ggataccgag gtggtggcaa agctgctgga ctatcgctat aacggcagcc
      481 ccatccagac catctctgat gtgatccgtg acctggaggg ttcctatgcg ctgggcatca
      541 tcttcgctga ccatccggat caggtgtatg ctgtccgctg tgaaagtccg ctgatcgtcg
      601 gcgtgggcaa gggcgaaagc tttatcgcat cggatgtgcc ggcaatcatt gaatacacca
      661 gagactacta cctgctggaa cacgaggaga tcgctgtgct gggcagcgac ggcgtgcata
      721 tctatgatgc cttcggtcag ccggtggaaa aggaactgaa aaccgcagac tgggatgtgg
      781 aggcgatcgg caagaacggc tatgcccact tcatgctcaa ggagatccac gagcagccca
      841 ctgccatccg gaacaccatc ctgccgagaa tccaggacgg cttgcccaat ctggaagagt
      901 gcggcatcac cccggagatg ctgcgcagtt tccggcatat ccaggtggtt gcctgcggta
      961 ctgccatgca cgccggaacg gtgggcaaat atgtgattga aaagctggcg agagtgccgg
     1021 tcactgtgga tatcgcctct gaattccgct atcgggaacc gctggtgggc gagggcgacc
     1081 tggtgattat tatttcccag tccggcgaga ccgcagacag cctggcggca atgcgcctgg
     1141 caaagtctct gggtgcaaag acgctggcga tcgtcaatgc gatgggcagc tctatcgcac
     1201 gtgaggcgga tatgctgatc tatactcacg ccggtccgga aattgccgtg gcatccacta
     1261 aggcgtatat ggtgcagatc gctgtgatgt atctgtttgc ctttgaactg gcgctggctg
     1321 tgggaaagat cgacgagacc gagtgccgcc gtctggtgca gctgctccag gatacatccg
     1381 acaccattac cggcattctg gaacacaagg agcaggcgca gtacatcgcc tcttctctga
     1441 ttaccgcaga cagcctgctg tatatcggac gtggtctgga ctatgccctc agcatggaag
     1501 gctctctgaa actgaaagag atctcctata tccactcgga gtcctatgct gccggcgaac
     1561 tgaagcacgg caccatctcg cttatcacgg aaaatatgcc ggtgattgcc gttgccaccc
     1621 aaaaagcgct gatggaaaag accatcagca acatcaagga agtcaaggct cgtggcgcaa
     1681 aggtgatcct ggtgtgccgg gaaagcgaca atgtagaccc ggatgctgta gatctgaagt
     1741 tcggtctgcc ggagttcgac gatctgctca tgccgatgat cgccgtagta ccgctgcagc
     1801 tgatcgcata ttacacctct gtcctcaagg gcaacgatgt ggataagcca cggaatctgg
     1861 caaaatccgt gactgtggaa taatagaaaa ggaacgataa cagtgatgaa atacaaaaag
     1921 attgcgctgc tggctgcggc agcactgaca atgacacaga tgctgggcat ttcggcggca
     1981 gcggaagagg atgtctcttc tgcggaggac agcgtggtag acgcagacag ccttggcagc
     2041 gatgagagca gcgaagaatc gtttaccagc ggtgactata cctattccct ggacggggaa
     2101 aacgccaaga tcacaaagta caacaacagc gatgaaacaa atgttgtcgt accggacgaa
     2161 ctggacggtc acaaggtggt ctctctgggc agcggcacgt ttgccacaaa ggataagatc
     2221 gtctcggtga cactgccagc ggcactgaca gagatcaaca gttcctgctt ctacggctgc
     2281 accagcctgg aaaaggtaga actggcagag ggcaacaccg catttaccgt gacagacggc
     2341 gtgctgttct ccgctgacgg cgaagatctg atcgtctatc cggatgcaat ggaaggcact
     2401 tcctatacca ttccggacgg ggtaaaggaa atctggacat ctgccttttc caacaccaag
     2461 ctgacagacg tcacattccc ggacggcgtg ctgtacattg acgactgggc gtttgcctct
     2521 tcggcgctga cttcgctgga tctgccggac tctgtgacgg agatcggaca gtatgccttt
     2581 gcctactgtg agggcatcca gaagatcgac ttgccccagg atctggaact gatccaggcg
     2641 gcagcctttg ccggtgagac cagtctgaca gaggtcacca tgtatgacag tttgacggag
     2701 atccagatgg cggcttttgc cgggaccggt ctgaaagaag taactattcc ggagagcgtc
     2761 acctatatcg gtttctgcgc attcggctat gaggcagata tgaccactaa ggtagaggac
     2821 tttgtgatct acggcaaagt cggctcccag gcgcagacct actgcaccgc agtggattcc
     2881 gaaaacgact attccaataa cttcaagttc cgcagcgtca tgtccgaaga ggtctccgga
     2941 agtgacagca ataccgtaga agtgaccgag aaggaaaacc tctggcagag ctacggcaga
     3001 tggatcctgc tgggcgccgg cgcagttgtt ctgctggtgg gcggcggcat tctgatcttt
     3061 gccggcggcg gaaagaagaa aaagactgcc tcttcgaaga aaaatgacaa ggcagaggaa
     3121 aagacagaag aaaagacaga agaaacagag acagagccgc acgaggatac caaatgagag
     3181 ctgtaacgac aatgatttgc gggatgctgg gggcagcgct gctgagtgct ccggtgatgc
     3241 tgccggcatc ggcggcaatg gcggagagca gccaggtttc agaaaccgat gacagcggca
     3301 tttccattga tacctccgga gacacctcct atgatgtgtt cacctatcgg gaaaatgaca
     3361 caggcgtttg catcacatcc tgtgataccg ccgccacatc cgccgatatc ccggaggaga
     3421 tcgacggaaa gccggtcaca gagatcggcg atgcggcatt tatgaactgt gcctatctga
     3481 ctgccataga tgtaccgtcc catgtgacga agatcggcga aagtgccttt tccagctgtc
     3541 ccatgctgtg ccaggtctct ctgccggagg ggctgacaga actgggcaag ggcgtgtttg
     3601 agtcctgtac catgctgagc caggtgacgc tgccgtctac gctgacagaa ctgccggagg
     3661 cggcattcta caattgcagc tatctgccca gcttttctgt gccggcgacg atcaaagtca
     3721 tcgggaacga ggcattttat agctgtactg ccttgaaaga ggtgacgctg cctgagggcg
     3781 tggcgtccat cggggactat gcgtttcaga actgccaggt gctggagcag gcgaaactgc
     3841 cggcatcctg taccactctg ggcaagtatg tgtttgacgg ctgccaggcg ctgacggcgc
     3901 tggatgtggc agagggcaac acggcctatc tgtcgcagga cggcgtgctg tttaccaaag
     3961 acggcgagac cctggtgcgg tatccccagg caaaggcgga gacggcatat accgttccgg
     4021 agggctgcag gacactggcg gactggagct ttattggctc taccacactg gaacagatca
     4081 acattgacgg ggtgacttct atcggtgagg actgtttcta ttactgcact tcgctgaaaa
     4141 gcatcgttat cccggacggc gtcgcagaac tgaacggcgc tgtgttcggc tactgcctgt
     4201 ccctggaaga ggtgaaactg ccggcgacca tctggaaact gggagatcac tgtttttatt
     4261 cctgcgccaa gctgaacaag atcgacctgc cggagggact gactacgctg ggagaccagt
     4321 gtttctacaa ctgcgtttct ctgctggaaa tgaacctgcc gcagaccatt acggagatcg
     4381 gcgaccaggc gatcgggtat tatacgccgt cagattccaa ggacaatacg gcgaagaaga
     4441 tcgaccggct gcacgtttac aacgccggca gcaatgcggt cagccagtat atccggcagt
     4501 ggaaatcaga cgactataag tattggatca ttagtggtgc ggtcgctgtt gtgatcgtcg
     4561 ggggtgtcat cgcattgatc tcggtacggc ggtatcggaa caagatacgg ccgaccagcc
     4621 gccatgcgtc ggagacgaag cggaagaaga aataaggcat cacctgccgc agaaaggacc
     4681 ccaaaccatg aagcatttac attcatttgc acccaaacgc ctgcttgcag ctgccgcagc
     4741 cggtgtgctt tcggtatgtg tgctgctgcc tgccggaaat gttctggcag cagagaccac
     4801 gaccgacagc agcagcgaga cattcgatga cggtacgctg acttataaaa aactcagcaa
     4861 caccacggtt tccgtaacgg actgcgtgga gtctgccacc catatcagca tcatgccgaa
     4921 gatcgacggc tatgatgtgg tttccatcgg agaagaggct tttgccaact gcacctctct
     4981 gcagggactg accattccgg acagtgtcac agagatcggc tcggcagcgt tttacggctg
     5041 taccgctctg gaaagtctga ccgttccgga ttcggtgacc aagatcgaga gtggcacctt
     5101 ctttaactgc agcgctctga ccaatctgac gctgggcgga aagaccacgg atatcggcga
     5161 tatggcattc ggctactgca ccagcctgga aacggttgct ctgccggaaa cggtggaaaa
     5221 tatgggcaat caggtgttct actactgcac ggcgctggat gatatctcca ttccggataa
     5281 ggtgacggaa ctgggcagct atacgttcta cggctgtctg gcgttgaaat cgtttgaagt
     5341 gccggtgaac ctggaagata tcggcgcaat gtcctttgtg gcgtgtccgt ccctggagac
     5401 cattacggtg gcagacggca acgccaaata taccgcagtg gacaacgtat tgtacgacag
     5461 cgaggagagc atcctctatc tgtatcctgc cggacggagc gacaccagct ttaccctgcc
     5521 ggacagcacg ctggtggtct atgccggcgc attctttgct gccggaaatc tccagcagat
     5581 cacatttgac gaaaagctcc agtacatcgg cgagatggcg tttgactttt gcagcggact
     5641 caccagcctg aacattccga agggtgtgac cactatcggc acgactgctt tcgccgactg
     5701 taccggactg acatctgtca cattcgacgg cgcatccgat gaggacggcg gcgacgggga
     5761 caagctggag atcggcgact acgccttttt ctgtacggac aatctgaaag aagtccagct
     5821 gcccaagcgt gtctcctcca tcggcaagta tgcctttggc tgcacttcgc cgtctgacga
     5881 tgacgacagc gaggattatg tgaccgtttc ttcggactcc ggagacgatc tgaaagtcaa
     5941 ggcgctgagc ggattcctgc tgatcggcta caccggcgct gcatcggact atgtgaagga
     6001 ctgcgatgtg gacatcagct ttaagtcggt gaacttcaac tggaaagggc tgatcttctg
     6061 ggctgtgctg gttgtggttc tggcggcagt agtgctgatc gctgttcgca tcatccgccg
     6121 caccatgatg acagcggaag aaaagcttgc cctcaagtcg gcgcaggcag aacagaaagt
     6181 gccgctgatg cagcgtgggg aatcgtcaaa gaccgaagaa ccggagtttg acgacggtta
     6241 ccgcagcatt ctcgcagacg acgaggatga ggacgaggcg gaagaagtgg cagactatga
     6301 gcagaccatt tcgcacagcc agctgcacag catcggtcac gccgatgcgg cagaaccgga
     6361 ggagaaagca ccggaggaag agaacgaatc agaaaacgaa taaaacagat gtgaccattt
     6421 cagcggtatg cgtgttgtta taggtgaacg gccgttcgag gaaaacgaaa ggagtgaacc
     6481 ggcgtgcagg aacgtattct gatacgcaga ctcaaacaac accagccgca ggctctggaa
     6541 gagctgatcg agacatacag cccctatgtg gggacgatcg tgcgcaatat tatcggaaag
     6601 tatctgtcag aatcagatgt ggaggaactc actgcggatg tttttctggc agtgtgggaa
     6661 cacaccgacc agctgaaagc cggaaagctc accgcatatc tggcggcgat cgccagaaac
     6721 cgggcgaaaa accgcatccg tggatatcac gagaccgtga atctggagga catgatagag
     6781 atcggcagcg gcgaggatct ggaagacgcc atcgaccgga agatcctgag cgagttgctc
     6841 cgggatgtgc tggacacgct gacggaaaaa gaccgggaga ttctggttcg gtattactac
     6901 tgttatgaga acgtccggca gatcgccgag acgctttgtc tgagtgagtc cgcagtgaaa
     6961 atgcggatga gccgggcgag aaagagactc cagcaggaac tgatcgcacg aggatatgcg
     7021 tatgaggaat cgccggcggt gcagcagata cgctttgccg ccatcggaaa aggagctgtc
     7081 tcatgaaatg gaattgctat gatctgttcg atcactataa tacagaaaaa aacggtgtgc
     7141 cggcgctgaa agaacagccc atccgcacag atgcagtgct ggaacgggta caggcgcaga
     7201 ccgctgccca ttcccagccg cataagcgca gccgcatccg gatgaccaag tgggcagccg
     7261 gcattgcggc agcggcagtg ttggtaaccg gcggtacaac gctggctgcg gctgccggtg
     7321 tcggcggact ggatgccttt tttcacagtc tgaccggcga ggaaacgccg gaaaatccgg
     7381 gaaagctggc ggcgcttgtc gctacgccgg atgccagctt cgacagcacc aaccaggacg
     7441 tacagtttac cctgctggga atgtacggcg acgacagcca ggcgatgctg agctttcagg
     7501 tgactgccaa ggacggcacg gcattgcagg acggtttcaa gctgccctat cagctgacct
     7561 tgcagggtac tgacggcagc agcgaggcgc tgggctgtta cggcaaaacg gcagatatcc
     7621 gggaaaaaga cggggcatat tacatcaatc tccgtatcaa ccgcaccgac ttgcagggca
     7681 aaacactgga tgtgaccttc cggaacttct acacggaggc gcagatctct cagatctggt
     7741 cgcagctgac agcctatgac gaccagctcc agcaggacta tgttcgtcag aacttcgggg
     7801 aagatgcgct gaaaggctgg ggaaacggcg aacttccggc agaatttgat gtggatgcct
     7861 ggaaagccta ccgccgggag aaccatctgg atcagaagtg cagcgaccgg gaaaaggaac
     7921 tctacgcaga gacagacggc gtgatctccg gcagctggca caccagcgtg acactggatt
     7981 tcgccagcgc agcgcccatc acggcggagt ttgacggcgg cacagtgaca ttgcagaccc
     8041 tgtccgcaag catcgctgca ccggcagaat gggacgacca gaaccatgcg gtcaactatg
     8101 ttatcacgct gaaagacggc agaaagatct tcgacgaact gatggcacag tacacagccg
     8161 gtgacgaaat gttacagcgt atccatgcgc tgcccggctg tgagacggat accatcgagc
     8221 agtatgtgcc gctggcagag aactactggg agaaggacag cgccacagac gtattttgct
     8281 acagcgaacc catcgacccg gcagaggtgg agacgatcac agcgtatcgc tttgcttttg
     8341 caggtgagga ttccgacggc accagcggac tgagcggcta tgatctcacc gaccgcactg
     8401 tgatctacag cgcacagtaa ttcgcagaga aaggatgttt caacatgagc agaaatcgac
     8461 agcacagccg caaacggcaa aaacatttga tgacagcagc gctgctgctc tgcctagcag
     8521 tgctggggtt ttcggtatgg ctcctgtttg ttcccaatca tctcaatcac gtgggagaaa
     8581 catccgtttt gcagagcagt acagctgatc ccggaaccgc agctgtgacc agtgattccg
     8641 gcacggtgca gaattccgat gcacaggcgt ttctggcggt gctggagggc aatactgcac
     8701 agctttccgc cgggaagtgg aagagcagcg acgagtcggt ggcaactgtg gacagcaatg
     8761 gaaccgttac cggcgtgaaa cagggcagct gtcagatcac ggacggcagc agcacctatc
     8821 aggtggatgt gcgccgtctg gaacagcggg aagagggaac gtatgtggac ggtattctga
     8881 tcgtcaacaa gtcctatccg ctggcggaaa actacgaccc cggtttgcag ccggtgacaa
     8941 aggatgcttt tcaaaagctg agcgatgcgg cggcacagga agggctggat atctatatcg
     9001 gttcggatta ccgggactac gcctaccagg tgaagatcta taacaactac tgcaatttat
     9061 acggctggga acaagcggac agcttttctg cccgtcccgg ctattcggaa catcagacag
     9121 gactcaccat tgactgcaac accattgacg atgccttcgg tgatacccag gaatccgtct
     9181 ggctggcaca gcactgcgcc gactacgggt ttatcatccg tttccccaag ggaaaggaga
     9241 gcatcaccgg ctatcagtat gagccgtggc acatccgcta tgtgggcgtg gacgtggcaa
     9301 aagagataca gaaatacggt ctgaccctgg aagaatatct gggcgtggat tccgtctatg
     9361 ccgaaccgtg gcagggataa acatactttt caacttgata gaaaagccgt actggaaagt
     9421 acggcttttt tgtgcctctg tgaaatcctt gttttccaat tctgtgaatc atccgcaaaa
     9481 aaagttgaca tatttagaat actatgttat aataaaacct gtgagaacaa aatcacagga
     9541 aagtgaatct tacggcagta tatgacgagt aaaaatgaaa aaggggcgat cgtatgaaag
     9601 cgtgtaaaaa gctgacggca cttctggcgg cattgacgat gctgaccgga acggcagggt
     9661 tgccggtttc cgcagaggaa atgactggca cattgggaga taccatgaca tggacagttg
     9721 acggggacac tgtgcgctgc acatgggaat ctgcagcggc ggacggtgtg gagatttcga
     9781 tacagggcga tacctgtacc attgaaaagg gcgtttattc ttgggaagaa tatcaggaat
     9841 ggtcgaatgc ggcagcaaac gaattgacgg aactgctgga ggcaaacggc tatgatcctt
     9901 ctgcaatggg cagcgaggaa aagaacgcag ccatcgcaga actgatgccg gaggcttatg
     9961 cagtgcggac agcgtttacc ggcatcaagc acattgtggt ttccgatgga gtgacgcagc
    10021 tggatgcgcc gctgggattc ctcggatttg gggattcgga aacggtacag ctgggaaaca
    10081 gcgtggtttc catcggggac agcacatttg agaataccca ctgcacccgg atcaccttgc
    10141 cggacagtct gaaaatcatt ggaaatcaag catttcttaa cgccggcgta aaagaactca
    10201 ccattccggc aggggtggag gagatcggcg acatggcttt ggaaaccgat tccactctgg
    10261 aaaaagtgac cattctctcc cgtgatgtgg acttgacgga taccggactg ggctatgtgt
    10321 ccgtctggct ggaaaccaat ccgtaccgca atgaaaatct ggtgatctat ggctatgccg
    10381 gttctactgc ggaacagtat gcggcggaca atgccattcc ctttgtgacg ctggcagaaa
    10441 cgccggtcta cggggatgtg aacctggacg gcagagtgga tatccaggat gccgtcctgc
    10501 tggcaaaggc agccgccgga acggtgacgc tcaacagcga cgccaaggta aacggcgact
    10561 gcgaccagga cggtacgatc agccaggcag acgctgcgat tctgatgcag tttctggtgc
    10621 atctagtaga tgaactgccg gtgcagggtg cataagtata atttggggaa aacgaaaaca
    10681 ggacagcgtg tcgaaattga aatgcgctgt cctgcttttt ttatgtttca gaatcgtctg
    10741 tttcggtggg cgtgcagctt ttcagcagcg cctgcaattc agaggtgaac cgctgtgcct
    10801 tgtccttgcc ccagtagcct acgctgtaca ggtggatgct gccgccggcg gcaggggaaa
    10861 cggtgaagtc gccgcagtct gacacctggt acagctggtc gccgctgcgc agtagaaagc
    10921 tggtggtgtc tatgtccatg gtgtagcttt ccaggggcag cggcgtgaga aagctgcggt
    10981 gataggtgta gccgtccagc agggaaagca gtgcgtcgta ttcctttgtt ccgggctgga
    11041 agtcataggt cacgctttct gtggtggctt ttcccgtgac ggaaacgccg gcggtgacta
    11101 ccgagaggga aaacggctcg tcggcgtgca tggcttttcc gatggggaac ggtgtcagca
    11161 gataccatcc gcctgccaga agaatgcaca gcaccagcag ccccagaagg acacgtttta
    11221 cgggtttggc agaacgcatg gcaggtctcc tttctgtaaa gatcgttgta tgcttgttct
    11281 attgtagcat attctgtcga aattgtaaag gcagtgcggc acaaatcttt tctgaaaaat
    11341 acctcttgac aagtgtactg caatgtgcta tactgtacct attgaatcag tacaatcagc
    11401 acagtgaaag ggggagagcc tgtggagatc attctcagca gcaacagcag caaacccatt
    11461 tatacccaga tcacggagca gatcaaggcg cagatcatgg acggcacact gcgtgccggc
    11521 gatccgatcc cgtctatgcg cagcctggca aagtctctgc acgtcagtgt aatcacggta
    11581 cagcgtgcct acgaggaact gcagcgtaac gggttcatcg aaacgaccgt cggacgtggc
    11641 agtttcgttg ccgtgcagga tccggcgttt tatctggagg aacagcagcg ccgggcggaa
    11701 gaacatctga gtgcggcggc agagatcgca cgaaccggcg gcataccgct ggaacagctc
    11761 attcgtgcgc tgaccatatt ctatgaggag gagaactgac catgcagaac attttagaga
    11821 tacgggatct ggggcgttcc tatgtgaatt tccggctgga acacatcagc ttttccattc
    11881 ccggcggaac gatcatggga ctggtgggcg agaacggcgc cggaaaaagt accaccatcc
    11941 gtgcggcgct gggacttatc cggaaggatg ccggcgaggt gctgtactgc ggcgaaccgc
    12001 tgaccacgga cagtgccgcc atgaaagaac agatcggcgt ggtgttcgac agcatctgct
    12061 tttatccgga actgacggtg gaaaaggcag ggaagatctg ccggaaaacc tatcggaact
    12121 gggatgaggc atatttccgg gaactgctgg agcagttttc tctggatccc aagcagaaga
    12181 tcaaggcgct gtcccgtggc atgagcatga aactttccct ggtgctggcg tttgcccacc
    12241 gtccgaaact gctgattctg gacgaaccca ccagcggact ggacccggtg gtacgggatg
    12301 acttactgga cttgtttctg gactttgtcc gggacgagga acacgccatt ctgatctcgt
    12361 cgcacatcac cagcgacctg gaaaagatag cggattatgt gacatttctc catgcaggaa
    12421 agttgctgct gaccaagccc aaggacgaac tgctctggaa atacggcgta ctgcgctgcg
    12481 gcgaggagca gtttcagaaa ctggacgggt cagaggtgct ggcatggcag aaaaaggact
    12541 attcctatga cgtgcttgtg ccggatcggg aagagctgct gcggagatat ccggaggcgg
    12601 tagtggacag agcaaccatg gacgagatcc tgctgttgta tgtgaaagga gcgcagaaaa
    12661 aatgaaggga ctgctcttga aggacttcta taccatatgg aaagccggac gaatttttat
    12721 tattctgtac atctttatcg cagccgtttc cggcatgaat ctccagaacg gctggtctta
    12781 catgggtttt tggatggtga tgatggatgc gcagacttta agcacctttt cctatgacca
    12841 ttcctgcaac tggaatcgtt ttgcactgac gatgcctgtt acacgaaaag acgtggtgca
    12901 gtcgaaattc cttttgcagc tgctgatcgt attcggcagt atgctggcat accttgtgct
    12961 ggctgccctt gtcagtctgg tgctgcggac gtttgaagtc aaggcgtttt gcaatatgct
    13021 gcttgccgca tggctcgtgt tctgggtcgt gctgctcatg ggcagcgttt ccacactgct
    13081 gctgttccgg tttccgccgg aatacgtgcg gctgatctcc attttggtct atgtgattcc
    13141 ggcgtgtgtt ttcatgggtg caggcagcat tttcgttcag gaaatagcgg acaatcctgc
    13201 cgtcctgcct gcggcgtttt ccgcaatgct gctgccggcg gcgattgcca tacccatact
    13261 gtccctggtg gtctccggac tgtgctattt cttcagctgc cgtgtctttc agaagaaaga
    13321 tctgacataa cgggaaagga gcgttttgca tgaaaatcat tgcccatatc gaaaatgact
    13381 tttccaccaa gttcggcatt ccccggcaga gtggannnnn nnnnnagtgg actggtcaac
    13441 agtctccgtt cccggatcgt ctttgcgccg gaataccgga atcccgatgc gttccgggga
    13501 ctggaggatt tttcccacgt ctggctgatc tgggagtttt cccaggcggt gcggcagaag
    13561 tggtctccta cggtgcgtcc gccaaggctg ggcggcaata cccggatggg cgtattcgcc
    13621 acccgttcgc cgttccgtcc gaatcccgtc gggctgtcgg cggtacagct ggaagaagtg
    13681 gtgctgcacg gcgcagatgc tccgtatctg gtggtatccg gcgcagacct gatgaacggc
    13741 acgcccatct atgacatcaa gccctatctg ccccatatcg acagccatcc ggatgccaga
    13801 ggcggctttg ccgttccggc ggcggaacat cggctgaaag tggtatttcc ggaacagtgg
    13861 ctggaaaaag tcccggaaca gctgcgggac gggctgaccg aagtgctggc gcaggacccc
    13921 agaccgtcct atcagcacga cccggaacgg atatacgggt tcggctttgc ccggctggaa
    13981 gtgaagttta ccgtagacgg ggacgtgctg acggtgtgcg gcgtgacggc acagaagtga
    14041 tctgcggcgg catcgtgtgt ctcagcaccg ccaaatctca cattttctgc aaaaaatttc
    14101 ggaaacccct tgcaaattgt ttcgttctat ggtatgataa atctgtacag ttcagagcct
    14161 gtatcaactt cgtggtacag gctcttaccg tatctgtgcg gactgccgtg cagatgcaga
    14221 aatcaacacc gtaaaatatt gataaaagga gatactactt atgcagtatg gttactttga
    14281 tctgaaaaac aaagagtatg tcatcgatca tccggatacg ccggcaccct gggtcaacta
    14341 cctgggttcc ccggaatacg gcgcaatgat ctcgaacaac gctgccggct acagctttgt
    14401 caagtctggc gcaaacggca gaatcagccg tttccggttc aactccatga tggcgctgcc
    14461 gggacgctac atctacctgc gtgacaatga caccaaggac tactggtctg ccacctggca
    14521 gccggtaggc aagccgctgg atgcctacaa gagcgtctgc cgccacggca cagcatatac
    14581 cgtgatttcc gcagactatg ccggcatctc ttctgaggtc acatattatg ttcccatcgg
    14641 cgctacctat gaggtatggc gtgctgtcat caccaacaac agcgacaagc cgagaaacct
    14701 gtctgcattc ggtttcgtag agttcaccaa cgacaacaac tacgagcagg accaggtcaa
    14761 cctccagtac accctgttca tcacacgcac cagctttgag ggcaacaaga tcctccagca
    14821 catcaacgag aacagcggca aggatgctac cggttccaac catagagagc gtttcttcgg
    14881 catggtcggc gcaccgatct ccggctataa cggcaatctg gacagcttta tcggtccgta
    14941 ccgcacctat tccaacccca tcgcagtaga gcagggcaag tgtaccggtg agatgaacta
    15001 caacggcaat gcctgcggcg cactccagag cgatatcacg ctccagccgg gcgagtccaa
    15061 ggaactgatc ttcgtactgg gtcagaagga caacacccag gcaaacgaga tcctggcatc
    15121 ctatgagact gccggcaagg tagacgaaga agtcgaggca ctgaaggctt actggcacgg
    15181 caagctgaac cacttccagg tagacacacc gtccgagtcc ttcaacaaca tgatcaacgt
    15241 atggaacgct tatcagtgtt tcattacctt tatctggtcc cgtgcagcat ctttcgtata
    15301 ctgcggtctg cgcaacggct acggctatcg ggatacggta caggatatcc agggcatcat
    15361 tcacctggat ccggagcttg ccgcagaaaa gatccgcttt atgctgtctg cacaggtaga
    15421 caacggcggc ggtctgccgc tggtcaagtt cgaccacaat gccggccatg aaaacacccc
    15481 ggacgatccg gaatatgtca aggagacagg tcacccgtcc taccgtgcag acgatgcgct
    15541 gtggctgttc ccgaccatcc gcaagtatgt gggcgaatcc ggcaacaagg cattctacga
    15601 tgaagtgatc gtctatgcaa acggcggcga ggatacggtt tacgaccatc tgaaacgtgc
    15661 gatccgtttc tccatggagc gcctgggcga ccacaatatg ccggcaggtc tccacgcaga
    15721 ctggaacgac tgcctgcgtc tgggcaagaa gggtgaatcc accttcgttg cattccagct
    15781 gtactatgca atgtccatga tgcacgagct ggcagcagag cgcaaggatg acgagtacat
    15841 cgcttatctg gacaaggtac agaaggcgct gggcgaagca ctggctgcct gctgggatga
    15901 ggaccgcttt atccgtggca tccgggaaga cggcgtagtc gttggcgcaa agaaggatcc
    15961 ggaggcatcc atgtggctga atcctcagag ctggagcgtt atctccggtt tcgcctccaa
    16021 ggaacagagc gacaaggcaa tgcagtctgt tgcagatatc ctggataccc cgtacggtgc
    16081 aaagctgctg gatccgccgt atatcgacaa ctatttcgac ggcgcactga tgcacatttt
    16141 caatccggat accaaggaaa acggcggtat cttctcccag tctcagggct ggctgatcct
    16201 tgcagagagc ctgctgggtc acggtgacga tgcgttccgt tacttcgagg aaagctctcc
    16261 ggcatccatg aacgacaagg cagaacagcg tgtgctggaa ccgtatgtac acggtcagtt
    16321 caccgagtcc acagcatccc cgtatgcagg acgttctcac gtacactggc tgaccggtac
    16381 tgcatccact gttatggtcg gctgcgtaga gggtatctgc ggtatgcgtc cggatgcaga
    16441 cggtctgcat atctgcccgt ccattccggg tgcatgggat ggtttcacca tggaaaaatt
    16501 cttccgtggc aagcagctgt ccatcacagt caagaacccg aatcacgtac agagcggcgt
    16561 tgcatctctg acactgaacg gtaaggcact ggaaggcgac tatgttccgg ctgctgcact
    16621 ggctgaacag aaccagatcg aagttgttct gggctgattc cagaaataaa ccatagaaaa
    16681 cagaaaaaga ccgccttttc ccggcacacc gtgggaaaag gcggtctttt gtgcgctgct
    16741 gcttgtatat tttccgccgc tgcgggcata ctctctgcga acagaaactt tttgttgtca
    16801 gttcctcttg acaagggaga gattctgtgg tacaataaat ctggttagag ataactaact
    16861 ataaaatgag agtacgtaaa ggagtcgtat agcaatgaat tacttgcaga tcgaaaaggt
    16921 tattggcaga gaaattctgg attccagagg aaaccctacc gttgaggcgg aagtgactct
    16981 ggttgacggt actaccgcaa gaggtactgc acccagcggc gcatccaccg gcgaattcga
    17041 ggcgctggaa ctgcgtgacg gcgacaaggc acgttatctg ggcaagggcg tgaccaaggc
    17101 agttgaaaac atcaacacca tcattaacga gactatcact ggcatggatg cctctgacct
    17161 gtacgctgtg gacaaggcaa tgatcgcagc agacggcaca aaggacaagt ccaagctggg
    17221 cgcaaatgcg atcctggcag tttccatcgc tactgcaaga gcagcagcaa ctgcactgga
    17281 tattccgctg tatcgtttcc tgggcggcgt ttccggcaca cgtctgccgg ttccgatgat
    17341 gaacattctg aacggcggcg cacacgctac caacaccgta gatacccagg aattcatgat
    17401 catgccggtt ggcgcaccca gctttaagga agcgctccgc tggtgtgcgg aagtattcca
    17461 cagcctggca aagattctga aggcaaaggg tctggctact tccgtcggcg acgaaggcgg
    17521 ctttgcaccg aacctgtcca gcgacgatga gaccatcgag accatcctgg aggcagtaaa
    17581 ggctgccgga tatgagccgg gtaaggactt catgatcgca atggatgctg catcttctga
    17641 gtggaagagc gaaaagggca agggattcta tcaccagccc aagtccggca gagacttcac
    17701 ttctgatgaa ctgatccagc actgggcaga cctggtagaa aagtacccca tcatctccat
    17761 cgaggacgca ctggatgaag aggactggga aggctggcag aagctcaccg caaagctggg
    17821 cggcaaggtg cagctggtcg gcgacgatct gttcgttacc aacaccgagc gtctgaagaa
    17881 gggcatcgaa ctgggctgcg gcaactccat cctcatcaag ctgaatcaga tcggttctgt
    17941 ttccgagaca ctggaggcaa tccagatggc acacaatgca ggctatacag caatttcttc
    18001 tcaccgttcc ggcgagaccg cagatactac catcgctgac ctggcagtag cactgaacac
    18061 ctgccagatc aagaccggcg ctcccagccg ttctgagcgc atggcgaagt acaaccagct
    18121 gctccgcatc gaggaagaac tgggcgacag cgcagtatat ccgggcatgg cagcatttca
    18181 cgtaaagcgt gactgagaac aaagctttcc tttctgagtg gatgttccac tcatttcaaa
    18241 ctcttctatg acggtaccgc ctggcggaac tgtcccctta aaccttatga aaaagcggcg
    18301 tattccggtt ggagtgcgcc gttttttctg cggcttattt ctccgggagt ctgtcgctga
    18361 cacgccagcc cagcagcacc agtcccagca gattgggaat cgccatcagt ccgttggcaa
    18421 tgtccgagag cgcccacacc gaccggagcg acaccaccgc accgatcccg gcgcacaggc
    18481 agaacagcag gcgatagggc agccgcagcc gctcgccgcc cagataccgc accgcctgtt
    18541 caccgcagca gcaccagccg atcagcgtgc agaatgcaaa cagtgctgtc agaattgcca
    18601 tgacctccgg ggcagcgctg ccgaaaatgc cggaaaatgc tgcggtgatg attgccccgg
    18661 cgtggtctgc ctgcgtcagt gcgccgctgg tgagaattgc cagcgccgtg agggtacagc
    18721 agaccagcgt atccagtgct acttctacca tactccacat tccctggagc gccgcatctt
    18781 ccgaactgcc gccggcgtgg accagggcgc tgctgcccag acctgcttcg ttggaaaaga
    18841 tgccgtggcg cagtccggtc tgtaccgcct gtttcagcac agcgccggaa ataccgccgc
    18901 ccacggcacg aatcccgaat gcaccgcaga agatgtcccg gaacgcaccc ggcagggcat
    18961 cgtgatgcgt tacgattacg ccgagcgccg ccagcaggta cacggcagac agcagcggca
    19021 tgagccactg gatgaccttt ccggtgcagc ttgtgccacg gagcagaatg atgcccagca
    19081 gtactgcaac gccaaccgcc gtccacagcg gcggcaggga aaacgcctgc tttgccagag
    19141 tggaaatggc gctgctctgg gtcatgttgc ccatgcctag ggatgcggca atacagcaaa
    19201 cggcatagaa cgccgccagt acccggctgt gcagtccgaa ccggagatat gccatgggac
    19261 cgctcactcg tctgccgtcc ggcagtgtcc gggcatagcg cagggcaagg acgttttcgc
    19321 tgtaggtcag cgccattccc agcagggcag agaaccacat ccaaaagaca gcgcccggac
    19381 cgcctataca gagcgccgcc gccacgccga tgatgtttcc cgttcccata gctgccgcca
    19441 gggcggtgga aaaggtctgg aactgggtga gggcagtgcg gttctcgccg gcgggtttct
    19501 ctttcagact gccgaaagtg cgtttcagaa tggtgcgcca gccccggaat tggagaaagc
    19561 gcagccggat ggtgagaaaa acacctgtgc cgagcagcag caccaatagt ccggcactcc
    19621 agatataaga tgcaatggtt tccagcatag cggcggtctc ctttcattgg acaggggcag
    19681 cagtgctttt gcgtaacaat ttctcatgca aatcgatttt tcggatagtc ccccttgaaa
    19741 agggggatgt cagcgtaagc tgacaggggg atagattatt acagagcatt tttcttagca
    19801 caaggcacat cttgtgggtg cattttcaaa gaaggcataa aaattgattt acgtaacaat
    19861 ttccccatat ctcttgacga aaatgccata tcggggtata atagtttatg aagatatcca
    19921 acccgtttag aagttgcggc ggcgcagcgg tgcgtgaaaa ggcacagccg tgtagtgccg
    19981 ccggagaatt ccacaggagg cgcagatgaa gcagtatact gccgacaaac acgcacaata
    20041 tactctgcag atcgctgcac ctgtggtgtc agcggtgctg attggattga tatggtactt
    20101 tttagcggta cttccccact ggatgctctg gacgctgaca gtcatcgtcg ctgctgctgc
    20161 ggtgctgctg tctacactgc tgctgcccat gtggttctgc accatcgtct acagcgtttc
    20221 ggcgacgcat atcacccgga agtgcggcat tttctttgta caggaacaga ccatgcgcac
    20281 ccaggcgctc cagttcagca gcatttttcg tgcgccgttt tcggaaaaga ccgggatgaa
    20341 cttcattccg ctccatgcct acggcggcac agtcattctg gcatttctga gccgctccga
    20401 tgcggaggag atccaggcgt ttctgcaaaa gacggtgtac agccacagcc agcgagtggt
    20461 ggaacagcct gtgttgccgg aagagggacc ggactttgac gtattcgaaa gcgccatgca
    20521 ggagacgaaa gcacctccgg cagacgacac ggaaaggagc gagccatgac ccggacggct
    20581 gcggcatccc cggcagttta ccgggagcat cccatcaaga tcctgcggta cagcgccaaa
    20641 aacctgtggc tgttgatttt tccgctgctg cgcagtctgc ggttttatcc gttctcgttt
    20701 cagaatctca tcgactgggg cgccggcgtg tggtttgacc tgctggtggc gctgctgatc
    20761 ttggggatcg gaacgctccg ctggcacgcc tgcatctact atttcgacga agtgaccatc
    20821 cgttcccgga gcggcatcct gctgcagcgt gtcacggaga tccctctgga gcgcattgcc
    20881 tccaccgtag aggaacaccc cttttaccta cgtccgctcc gggcggcgtg tctgcgcatc
    20941 agtacagcgg caggcgcaat gccggatgcg gatatgcagc tgacgctgta tctgcgtgac
    21001 ctgcaccggc tgcggcagaa tatcccggtg ctgcaaaacg gcaagtcgga agaggcggca
    21061 tatcacacgc cggcgtggcg gacgatgctg ttctcggcgc tgttttccag cagcttttcc
    21121 ggggcgctgt acattgcgac cctttttttt cagggcggcc gtatcacgtc tgacctgctg
    21181 gaacagttcc aggcgcagca gctcctggag gatgccacag acagagcgtc cacggcgttc
    21241 cacggcgtac cacgcatcgc cgtcaccatc ggcatcatca ttctggcgct gtggctgatc
    21301 tcattcgggc ggaatctgct gcgctacggc agattcaaga tgcggttcgg cagtgagttt
    21361 atctcggtgc atacgggcat catcacccgg cggcggtatc atctccggga caccgccatc
    21421 atctttcccg acctgcggca gaacctggtg atgaagattt tcggcatggt ctcgctgcac
    21481 atccgctgtc ccggctacgg cagccggcgt gacaccctgc cggtgctgat tccgctgatg
    21541 cgcaaaaaaa gctctcagcc cctgctggaa cgcctgcacg ccgtgccggt gatggaacat
    21601 ccgaaactgc acgcaaaatc caacatcaag tttttctggt cgttcgtctg gccgccggtg
    21661 atcggacttt gcgccatttt tccggtgcga tttttcctgc tgtggctgct gcccagtgtg
    21721 ggagcgatca tccgcttttt ttccgtcatg atgattattc cgctggtgtg gctgctctgc
    21781 atccgcattg tggcgatggt ctcggaatcg gtcaccatgg acgaccagca cctgcagatg
    21841 cacttttgca gctggttcac attccacacc atcacggtga accatgcacg catcgtccgc
    21901 acggacatta tgcagacgcc catgcaaaag cagtatggag tttgccattt atatatcacc
    21961 tgcaacggac cccggcagca gcggttcaag ctgacggcgc tcccggagaa aaaggtccgg
    22021 gagattctgg cgatgctgga gcagggagca taatcacaaa aacattcccg gtttctgcgt
    22081 gggtgcggaa accgggattt ttttgtaaaa atgcagataa atcattgact ctttttcctt
    22141 tttgtactat aataaagata ttctgtttta tcaatatgat atggaggcat gaaaatgcaa
    22201 ttgtggaaaa aggcacttgc ggcagtgact gccggggtgc tttgtctggg tagtgttggc
    22261 atttctgggg tgcagagtgt gctggaaagc gtgagaacgg tgctaactgc gagtgcagat
    22321 gtgccaggtg agtatgatgg aacttatggg gatttgtact ataagaacga tgatgatttg
    22381 gtttctatag tcggttgtaa gacaactgct gagtctgtag aaattccggc agaaattgac
    22441 ggaaaacctg tgacaagtat tgaaaaaatg gctttttccg gctgtaccag tttgacggca
    22501 atcacgatac cggacagcgt gatgagtatt ggcgatagtg ctttttccaa ctgtaccagc
    22561 ttaatagaaa tcacgatacc ggacagtgtg acgggaattg aagaatatgc tttttccggc
    22621 tgtaccagtt tgacagcaat tacaatacca gacagcgtaa caaatattgg agtgggtgta
    22681 ttttttgact gtaccagttt gacagaaatt acgataccag acagcgtgac aagtattgga
    22741 tatactgctt tttgcggctg taccagtttg acagaaatca caataccaga tggtgtgaca
    22801 agcattggag gggctgcttt ctctaaaaca ccatggttag ctgaaaagca aaaagaaaac
    22861 ccattggttg ttgtgaatgg aattttgatt gacacagaaa cttgcaccga caatgttgtt
    22921 ataccggaca gcgtgacaag tattggagag tttgcttttc acggttgtac cagtttaaca
    22981 gcaatcacga taccagacag cgtgacaagt attggatgtg aaacttttca tgactgcacc
    23041 aatttgaaaa atgtttatat ttctgattta gaatcttggt gtaagattga tttttgtggt
    23101 actacttcta atccattgca ttgtggtgcc aatctttata taaacggaga gttggtaaca
    23161 gaaattacga taccggatgg cgtgacaagc attggagagt ttgctttttc cggctgtacc
    23221 ggtctgacgg agattaccat tccagacggt gtgacaagta ttgaacgtta tgctttttat
    23281 ggttgtgaaa atttgacgaa aatcacgact ccagatagtt tgataagttt tgggtataat
    23341 gctttttggg ggacgccatg gcttacaacc aaacaagaag aaaatccgct tgtgattgtc
    23401 aatggggttt tgattgacgg gacaacctgt actggaagtg ttacgatacc ggacaatgtg
    23461 acaagcattg gagaatgggc atttttttat tgtgagaaat tgacgggaat cactattccg
    23521 gacagtgtga tgaatattgg atgggatgct ttctctcatt gcactagctt gacggaaatc
    23581 acgattccaa atagtatgac gagtattgga atgcaagctt tttacggctg taccaatctg
    23641 acgaaaatta ctattccaga aagtgtgaca agtattgaaa gctttgcttt tgaagattgc
    23701 aataacgtga caatctacgg ctacaccggt tcctacgcag aaacctatgc tgaggaaaac
    23761 gacattcctt ttgtctctct aggcaacgct acaacaacag aaaccaccac tactacaacc
    23821 gaaaccacca ccgaaacgac tgaaacaacg actactacca ccaccccgga gaccccgacc
    23881 atcgccaaag gcgacctgga cggcagcgac cgcattgact ccaccgacat cttctacacc
    23941 atgtattacg tggcaaatgt tgccgtagga aaccccggcg gtctgacaac ggaacagatc
    24001 gcagccgccg atgtggacgg cagcggcaag gtggactcca ccgacgtatt ctatatgatg
    24061 tattacgtgg cactccacgg cgtcggcaag gatgtgtcct gggaggaagt cctcgccaag
    24121 taagaaaaat tcttagaacc aaaaagggct gcggtacttt tcacgagtac cgcagccctt
    24181 gctgtatccg gttacatttg taaaatctgc gctagtatct gcaggatctt tgccccgtag
    24241 ttcgccccgg atgcccagcc cttgtggttc aggttctcct ggatgccaag ccactccaca
    24301 taaggcgcac agccacgctg gacatagcgg aaacgggtgt cgatgcagtc ctgtttcagc
    24361 ggctcagtgg aggcgtaggc tttcagatgc tgtatctgcg cccggatgcc cagctggggc
    24421 gtggcaaagg aattgccttt cacgccgttt tgcgtcacgc ccatgccgca gaaattgttc
    24481 tggctcagcg tcacggcaga gccacggaag gtgaagtttc cggtctccag gcaggactgc
    24541 gcaaaggcaa tgtccccacg cacgccctct gcctcgccct cgctgagata gtaggggatc
    24601 atgtccagga cgctctgcgc tacgttgggg ttgacagagc ggatgtaggc ggccatctgt
    24661 gcgactgtcg cctgggactt gcccatgacc ggggtggtgt tggtggaaag cttgccgtca
    24721 tcggcggcac gataggtcac gccgtcgatg aggacggtct ggctgcgcac cagcttgccg
    24781 tcgctttcga agtaatagga actgccgtcc agctgctgca tccccaccag tgcggtctcc
    24841 gtgtccgggc tgaaatagta gatgtctctg ccgtctttct gccagccggt ctggatgctg
    24901 ccgtctatac tattataata ggagaccgtc ccgtcagcaa acgtgcatac gcccactgtc
    24961 tgggtgccgc agtccgggtc gaactggtac cggtgtccgt ccagcgtctg ttcgccggtc
    25021 agctttccgg tgtcgctgtc cgcataatag cggtgaccgc cggactccac ccagccggag
    25081 accggttctc ctgtgaccgg gtcgtagtag cggcgtatac cgttcaccgt gcgccagccg
    25141 gtcttttgcg ctccggtgta gtcgaagaga tagtccgtac cgtcgatggt cacttcgccg
    25201 gtgacgcatt cgccctgggc atccagctgc tgcatcccca ccagtgcggt ctccgtgtcc
    25261 gggctgaaat agtagatgtc tctgccgtct ttctgccagc cggtctggat gctgccgtct
    25321 atactattat aataggagac cgtcccgtca gcaaacgtgc atacgcccac tgtctgggtg
    25381 ccgcagtccg ggtcgaactg gtaccggtgt ccgtccagcg tctgttcgcc ggtcagcttt
    25441 ccggtgtcgc tgtccgcata atagcggtga ccgccggact ccacccagcc ggagaccggt
    25501 tctcctgtga ccgggtcgta gtagcggcgt ataccgttca ccgtgcgcca gccggtcttt
    25561 tgcgctccgg tgtagtcgaa gagatagtcc gtaccgtcga tggtcacttc gccggtgacg
    25621 cattcgccct gggcatcgta gtaacagcgg ctgccgcttt ccgtcacgcc ccagttgccg
    25681 gagggctggg tggtttctgt ggcaacggga acagtcaccc cggcgctgtc cgccgccgca
    25741 gaaaagccgc ccaggatgca ggctgtccnn nnnnnnnncg agggaaagca ggaatggaaa
    25801 atgtttcaag tgtggtcctc ctttgtattg tgttttgtgc ggtattgcct ttattgtagc
    25861 atattttcgg ggatgttgca accgaaaatg cacaaaaaat gtgccgcagt atgcagaaaa
    25921 gtgtcacatt ctccggactt ttcgtcaaac tgccggaatc ggacgcagaa ggacactgca
    25981 ttttttgcgc tgcgtgggaa aaaataacgc agactttgtt gacaattcgg caaaaatccg
    26041 gtataatgta gtggaaagag aatgttgcag gggaagatac tgatagggga acgctggcag
    26101 gtctgccggc agaaattcag ttgctgcaaa tgcgcagcag gaggaatcta tgtcgttaca
    26161 ggaaaaagaa gcatacgacc tggcaaaatg taagcttgca gaatatttgc aggacagcgc
    26221 cgtctgctcc atggatgaat acgtgcagca cggcacgacc agcacattac agcactgcct
    26281 ttctgtggtg catatcagct gtgccatggc agaaaagctg cacatccgtg taaactatga
    26341 aaatctgatc gtcggggcat tgctccacga cttttatttg tatgactggc acacgcacca
    26401 ggatgagggc gtgctgcacg gctttgccca tccgcacatt gcctgcagaa atgcagcgat
    26461 gcggtttcac gtcaacgcag aggtacagca catcatcacc acccatatgt ggccgctgac
    26521 gctgcggttt gtgccacgtt ccagagaggc ggttctggtc tgcatggcgg acaagtactg
    26581 ttctacccta gagaccgtgg tgggattttt ccacgctgcc gctgcactgc tccgaagaaa
    26641 gcaggtgtga gtttatggta cattatcttt ctgactatgt gatccttttc tttgcctatg
    26701 ccaccctggg ctggtgcatg gaaacattat atatgggcgt ccgggaacgg cgcatcacca
    26761 accgtggctt tctgctggga ccctgctgtc cgatctacgg cgcaggcatg gtgggcatta
    26821 ccctgttgtt ccaggacagc cactacagct attggcacgt gttcttttct atcatcctgc
    26881 tgtgcagcct gctggaatat gcagtaagcg ctttgatgga acgggtgttc cacactcgct
    26941 ggtgggacta tcacgatatg cggttcaaca tccatggccg tatctgcctg gagaccatgc
    27001 tgccctttgg tctgctgggg acatttgccc tctaccggat caatccgttt ctgatgatgt
    27061 tgtatcatcg ccttcccgga tttatttccg ggaaattggc ggtgggactg gtggttctgc
    27121 tgctggcgga tctgaccatc tccgtctgtg tcctgcggaa tctttccctg ccggacggcg
    27181 acgccgacca taccgccgag atctcacgcc gtgtgtggga agatctgaaa aagcggcttg
    27241 gctgaacaaa ttgaataaaa cttttttgtg cattccgctg tgttttagtg ggatgcactt
    27301 tttttgtgtg cgtcagatga aaacgacaaa atacacttga cagaataaat gtcatgcgtt
    27361 ataatagaac aaagttattt cataacatag ttatagataa aggaggcttt ggagagatgg
    27421 attgtgaggc aaacacgttt tttggcgtaa tgaatgcgtt tcgtgggttg aatgtgtttt
    27481 ctctgctgcc cctgtcccgg caggagtata tgctgatgtt cctggtgttc cgtcaccgaa
    27541 agaccaaccc ggagggctgt acggtatcga ccctgacccg gctgatgcgt gtgccgcagc
    27601 cggcagtgtc ccggacgctg cgtggactgg agaacaacgg ctacatccgc cgggaagtct
    27661 gccgggagga tcggcgcaac acgtatgtga cactgacggc tgccggagag acagaggtac
    27721 agcgtgccaa tcagcttttg gaggaatttc accggggtat ccagaaacga ttcacgcagg
    27781 aagagtccga tcagctgatg gaactgctgc ggcggctcta tatggcggca tcggaagaac
    27841 tggatgagat gaaaggagag aaacaagcca atgggtaagc ttttcaagaa cctgaaacct
    27901 tactggaaat ctgtgctggt gattttcgtg ctgttggtgg tgcaggcgtt ttgtgacctg
    27961 tcgctgcccc agtatacgtc ggatatcatt gacacaggca tccagaacag cggcgtagcc
    28021 cacatcctgc cggaaaagat cacggcggag gagtttgata atgcccagct gttcatgacc
    28081 gaggaagaac agaaccagtg gcaggatgcc tatgacggcg agaaccggaa agacagcctg
    28141 tcggaaaaga aactggacga actggacagc gacctgctgg tgccggtgct gctgaattat
    28201 cagatgtcca tgatgaatga ggacactttc cgccagatgc tggcgcagca gcagaccgac
    28261 cccaatgcgg aggcacagat ggaacagatg tctgtggaag aaatcggcgc tatgatgggc
    28321 gtggaactga aaccgttcca gcgcaccgaa gaggacgacg acggcaatga gaccacggtg
    28381 acctgtgtgg atgtacgccc ggtgtttgcg gcaatgcagc agagcggtca gctcacggaa
    28441 gagatagtga accagatgcg ctcccagctg gaggatatgc tggacactat gggcgactct
    28501 ctggtgaagt ccatgggcgt ggcatatgca gtgcagtgcg acaaggatgc cggactggac
    28561 gtggataaga tccagaccag ctatctctgg cgcaccggcg gcaagatgat ggcgatggca
    28621 ttcgccatga tggctgcggc gatctgcgtc ggctattttg catcccgtgt gggtgcaggc
    28681 gtgggacgtg atctccgtgg aagagtgttc cagcgtgtca tgcagttctc cagtgcggag
    28741 atggatcagt tctccaccgc atctctcatc acccgcagta ccaacgatat ccagcaggtg
    28801 cagatggtga cggcgatttt cctgcggatg ctgctgtatg cgccgattct gggcgtgggc
    28861 ggtattctaa aggtggtaca taccggcgca ggcatgggct gggtcattgt cctggcggtt
    28921 ctggtaattc tggggatcgt cggcgtgctg atgtccattg caatgcccaa gttcaagctg
    28981 atgcaggtgc tggtggataa ggtgaacctg gtctcccgtg agattctgac cggtctgtcg
    29041 gtcattcgtg cattcggacg ggaaaagacc gaggaagagc gtttcgacca ggcaaaccgg
    29101 aatctgaccc gaaccaacct gtttgtcaac cgtgtcatga cctttatgat gccggtgatg
    29161 atgctgatta tgtacagtct gaccattctg atcgtctggg tcggcgcaca ccgtgtggac
    29221 ggcggctcca tgcaggtcgg ttctatgacg gcgtttatca cctatgctat gatgatcgtc
    29281 atgtctttcc tcatgctgac catgatgtct gtcatgctgc cccgtgcatc ggtagctgcc
    29341 ggacgaattg acgaggtatt gcagacagag acttccgtca aggagaacga gacaccgaag
    29401 catctggaca cctgcaaggg cgttctggca ttcgaccatg tccatttccg ctatccgcac
    29461 gccgaagagg atgccctgtc cgacctgtgc tttaccgcag aaccgggcaa gaccacagcc
    29521 atcatcggca gcaccggctg cggcaagtcc acgctggtga atctgatccc tcgtctgtac
    29581 gatgccacag agggcagcat tacgctggac ggcgtggaac tgcgtgatct gccgctggaa
    29641 gaactgcggc agcagatcgg ttttgtgccg cagaagggcg tgctgttctc cggaacgatt
    29701 gccagcaacc tgcggttcgg caatccggat gcatcggatg cggagataga gcaggcggca
    29761 gagattgccc aggcgatgga gttcattgga gaaaaaccgg atcggttcga cagcgatatc
    29821 tcccagggcg gtaccaatgt ttccggcggt cagaaacagc gtctgaccat cgccagagcc
    29881 attgccaagc acccgaaggt gttcgtcttt gacgacagct tttccgcact ggatctgaaa
    29941 accgatgcgg cactgcgcaa agcgctgggc gagcaggtgc aggacagcac catgattatc
    30001 gtggcgcagc gtatcagcac catcctccat gcagaccaga tcctggtgct ggaagaggga
    30061 aagatcgtgg gcaagggtac acacgaagaa ctgctgcgca gctgcgaagt gtaccagcag
    30121 atcgcaaagt ctcagctttc cgcagcggaa ctgggactgg aagaggacac agagaaagga
    30181 gcggtgacag atggcgaatg aaagacggcg catgggcggt ccgagaggcg gtcagatgcc
    30241 ggtggaaaag gcaaaggact ttaagggttc aatcggcaag ctgtttgtgt atatgaaaaa
    30301 gtatcacgct gcggtagtcg tcgtgatcct gtgtgcagtt gcctccactg tgttcagcgt
    30361 cattggcccg gatatcctgg gaaaggcgac cacagagctg gcaaacggtc tgatggcgaa
    30421 gatccaggga accggcggca ttgactttac gaagatcgga cagattttgc tgttcgtgct
    30481 ggggctgtat ctgctcagca ccctgttcaa ctttgtccag ggctggctga tgaccggcgt
    30541 gacccagaag gtctgctacc ggatgcggaa agacatctcc gaaaagatcg accggatgcc
    30601 tatggcatat ttcgaaagcc gcacctacgg cgaagtgctg tcccgcatca ccaacgatgt
    30661 ggatacgctg ggacagagtc tgaaccagag cattacccag atcatcacct ctgtggcaac
    30721 tatggtgggc gtactggtca tgatgctgcg catctctccg ctgatgacgc tgatcgcagt
    30781 gattatcctg cccatttctg tgacgctgat ctctctggtg gtcaagaagt ctcagaagta
    30841 cttcaagcag cagcaggaat atctgggaca catcaacggt caggtcgagg aatcctatgc
    30901 cggtcagact gtgatccagg catacaacaa ggaaaaggac accgtgcgcc agttccagga
    30961 gacaaacgat accctgtatc actccgcatg gaagtcccag ttcctgtccg gactgatgca
    31021 gccgatcatg atgtttgtgg gcaacctggg ctatgccgct gtggcactgt ccggcggact
    31081 gctcgccatt cgtggtacca tcaccatcgg tgatatccag gcattcatcc agtacgtgaa
    31141 gaacttcacc cagcccatgc agcagatcgc acaggtgctg aaccaggtgc agtcgatggc
    31201 ggcagctacg gagcgtgtgt ttgaattcct ggaagaggac gaagaagtcc agaccgcaga
    31261 gcatccggta cagctggcag atgtcagtgg cagcgtggac ttcgaacacg tttccttcgg
    31321 ctataagccg gagcagtgca tcatccacga tttcagcgcc aaggtgcatc cgggacagaa
    31381 agttgccatt gttggtccta ccggtgccgg aaaaacgacc atggtgaagc tgctgatgcg
    31441 gttctacgat gtcaacagcg gttctatcaa gctggacgga cacgatgtcc gggactttga
    31501 ccggcatcag ctccgggaat ctttcggcat ggtactccag gacacctggc tgttccaggg
    31561 aacgatcatg gagaatatcc gctacggcag actggatgcc acagacgaag aggtcatcgc
    31621 cgccgcaaag gcagcccacg cagatacctt tatccgccag ctgccggacg gctatcagat
    31681 ggagctgaac gaggacgcca gcaacgtgtc acagggacag aaacagctgc ttaccattgc
    31741 acgtgcgatt ctggcagaca accgggtcat gatcctggac gaggcgactt cttccgtaga
    31801 tacccgaacc gaaattctga tccagaaggc aatggatcat ctgatggagg gcaggaccag
    31861 ctttatcatt gcgcaccgcc tttccaccat ccgcaatgcg gatatgatcc tggtcatgaa
    31921 ggacggcgac atcatcgagc agggtacgca cgatgagctg ctggcgaaga acgggttcta
    31981 tgcggatctg taccgctctc agttcgctca gacggcataa ccgcatttca aaaaaataac
    32041 agcataacac gcaaattggt ttttctccat tttgcctcct ctttagagga ggtgttccgg
    32101 agggacggag gagtcagaaa aatcaatttg cgtgtttttg cataaaaagg gcttgccaaa
    32161 tgaaaaagat tgtgctataa tgataatatc tgttgttctg tccgctggaa gggaacggcg
    32221 gaacacaccg cagcgccgtt tgcatccgat gagacgggct gtatctcact atactttaag
    32281 gaggacttcc atggcaacca ccaacaccca agccgtgccg ctgcgtacca agcgtggtat
    32341 cagtgccggc agcattttct cggcaatcgg aagatggatc gtccgaaacc ggatctatct
    32401 catcgccttt gccattccgg ctgcgctgac ctatctggcg tatgccatct tcggactcta
    32461 cccctttggc gaagaatccg tgctttgcct cgacctgaac ggacagtacg tctactattt
    32521 tgaagcactt cgtgatgcgt tctggggcga cggcagcatt ttctacaact ggtccagaaa
    32581 cctttccggt gaatttatgg gcattatcgg ctattatctt gccagcccgt ttactttgat
    32641 cgtgatgctt ctgccggaga agttcatgct gagcagcctg cttatcatgc agctttgcaa
    32701 ggtgggcgct gccggcgtga cattcaacta tttcctgcaa aagcgccgga acgtacagcc
    32761 ctatccgtcc ctgctgttct ccaccatgta ttccatgatg gcatatatgg tgatccagct
    32821 gatcgacccc atgtggctgg atggtctggt attcctgccg ctgattatgc tgggcatcga
    32881 gtatctggtc gatgacggca gacggctgaa ctacatcatt ccgctggcgc tgatgtgcgt
    32941 ggcaaacttc tacatcggct acatgatctg cattttcgtg gcactgtact tcttcttcta
    33001 cctgctggca ggttcggaca agaagcgcaa gggctatgac tatttcaccg ccatcgtgcg
    33061 tgtggcatat tcctcgatca ttgcactagc ttgtgcggca ttcatgctgc tgccggtgta
    33121 caatgcactg aaactgggta aattcgagtt tacggatccg gactatagtt tccggttcca
    33181 gtctttcccg ctgagtcaga aactgctgga aaatgcgcag gattcaccgc tgctggtact
    33241 tctgtgcgac ctgctggata tgttcccgca gctgctcccg gcacagtatg actctgttaa
    33301 cgtccagggt aagccggaga tctactgcgg tctgcttaca ctggtgctgc tgccgctgtt
    33361 ctacatgaac aagaagatca actggaagaa aaagctgggc tatacagcgc tgatcgttgt
    33421 gatggtaatg tgtatgctca tcacaccggt ggacatgatg tggcacggcg gacaggtgcc
    33481 gaactggctg ccgttccgct attcgttcct gctgtccttt atcttcctca ccatggcggc
    33541 aactgcattt gccaacaagg acggcatcca gaagaaacat ctgctcggct ctgccggggt
    33601 gatggtggtt atcatcgcca ttgttgccgg tctgaaattt gaccagatgg caaagggtgc
    33661 agtctggatc tctgcggcac tcatgggcat ctatctgatc ctgctgtact tcatgatcgg
    33721 cggcaagctg acagagggca agcgtggcgt gagcattgcg ctgacgacta tgatgctggt
    33781 tatggtcggc ggcgaggcaa cctataatgc agtggattcc atgaaggata tcgacgatga
    33841 ggtggcatat tccacccgtg catcctatca gaactatgtg cagaacggca gagcggctgc
    33901 ggatatgctg gaggaaaagg atgacggttt ctaccgtgca gaaaagactt tcttccgctg
    33961 tgtcaacgac aatgcggcgc tcggactgaa tggtatttcc cactccagtt ctgtcatgaa
    34021 cacccgggtc atcaacttca ttgagaccat gggctattgt atgcacagct actacacccg
    34081 ttatgacggc aacacagaga tcgctgactc tctgctgggc atcaagtatg tactggatcg
    34141 tggtgaaaac ttcgaccaga accgccgtct gaacccggcg tatgaacctc gctgggcata
    34201 tgactacaaa aacgaaaacg gcgtggacaa gaccatcacg gcgtatgaga ataccaatgc
    34261 gctgtccatc ggctatatgg tggatgaaga tgtacagaag atcgaccacc tgggcaatga
    34321 caatccgttc aacagccaga acatcttcat gagtaccatg agcggcaata cggaattcga
    34381 tgaaaacggt cagtttgcat cgtggcacga atactacaca ccgctgaacc tggcagaaga
    34441 tcccatcgtc agcgattcca ttaccaccag tccttacggc gaacagacca agtatgatgt
    34501 gaccggcgac ggtgacccga cggtggactt ccacctggtg gcagattcca gcgatccgat
    34561 ctatatctac ttcaagacag agttccagaa gaaggtcaac ctgtggatgt ccacctggga
    34621 cgagagcgag tggagcgcag acgaagagag ctggaaggca gaaaacgagg gtgacttcac
    34681 ctggaaggct gccgacgaat acgcaaaaaa tcacagcggc tttgagtctc tgggtgaata
    34741 cttcgagggc gacaactaca ttgccctgcg tctgggtact ttcgagccag gcacaaagat
    34801 cacgctgcgt atgaccattg ccaacgatga aaagttcacc atcgtgaaga acttcctgtt
    34861 ctacacatac catccggatg tgttccaggc agatatcgac acgctgaaga agaaccagtg
    34921 gcagatcgac tcttccaaga gcaacgaccg gaaactcacc ggtacgatca ccgcagaaga
    34981 tggtcagatc atgatgacct ctatcccgta tcagccgggc tggtccatca aggttgacgg
    35041 caagcgtgta gacaacctgg tagaagaggt caagaacgag gacggcagca ccagtctgaa
    35101 caatcagagc ggtgactccg gacagatcgt tgtggtagat gcaatgatcg gtctgcggct
    35161 gcctgccgga acgcataccg tgacgatgac ctatacaccg ccggggtggt ggatcggcat
    35221 gggtacgctg gctggcggta ttatcctgat tgtgctgttc tggttcggcg accgcaagcg
    35281 ctatgctgca atgctggttg cagtagaggc agaaaaggaa cgggaacgtc agcaggcaaa
    35341 gaaacgtgtc aagctgaagt ccaacaacaa taaggataag gacgattctc cggaaaaact
    35401 gctggaaaaa ctggaggcac tccataagga gggtattctg gacgacaagg agtttgcagc
    35461 caaaaaggcg gagattgaaa agaaaaagaa agcgaacggc aaacagacgt aattgtcagt
    35521 ttgtgtaaag tacgctgaaa aatgaaacgg cgttgccgca gggaaaatgt tcctgcggca
    35581 acgccgtttt tggtttggtg tgcgaaagga gatttttaac cgtaactata tgnnnnnnnn
    35641 nnactatatg acatcacatc acgataggat ggaaagaacg attctaacga tacaaccacg
    35701 attatcacca aaatgagaat cagtagactc accagagact gcgcttttgt atatcgtttg
    35761 attttttcag atgacctgga atatgcaaat atcatgccgg caacgcagag gacgaataaa
    35821 agtacaagaa acagaaaacc agcaaaattg ccgccggcag actgctttcc atagccttct
    35881 gtcatccagt ttagcgctct gacagaataa tgtgtctgcc aaaaatattc gaaacataaa
    35941 aaggaaaaag gcagcgtcag aacccatttc aacaagaggg ttttcctgtt ttcgcttaac
    36001 agcatggctg catatatcgc tgcgacaacc agcagatacg gaaacaggag caaatcgttt
    36061 gcggtacgga taatttccgc aagaaatatc gctgacacaa caacaactga cattgctgcc
    36121 gaacagatta tagtgtttat cgttttgaat ttcatctctt tctccttaaa tagggtggag
    36181 caatttttcg tgaattttga tttttcccat tggtttgatt ctgaaactac gccgtatcac
    36241 atagcagttg atatccaaat cataatgata tgttgcacat attcgcccaa tatagttaga
    36301 acgaaagaaa aggagaacaa gctgcttgtc agatataaaa acaagctcgc tatagcgtat
    36361 gtgtttgctt gatgaaacac aggaacactt atttcataat taaaccaaaa gccgttgata
    36421 ctataaattt agtgtcaatg gctttttcta tatataagtt tttttcaaaa agcgactcga
    36481 attagtcaga cttcacaaaa cagccaatag aaatcaagcg aagattcggc aaaagtaaca
    36541 gctcggcttt tcaaccgtta ataactgcgg acaactgtat cgttttttcg tgcagaaaga
    36601 ttttttcttt gctgaaaaaa caattattca atacccaaaa gaatatttac agctttcctg
    36661 aggtctggtt tggtgcgata agccataatt acctttcgct cttgttcgct taaatcttcc
    36721 ggtgataatc tgacatcaag cagctcactg ggagatacat tcaatgcctt gcagatatcc
    36781 gcaagaatat ccgcatcggg acggttgatt ccctgttccc agttggaaac acggctgttg
    36841 ctgacaccga ttttctgtgc cagttccttt tggctgaaac cactcatttc ccgatattta
    36901 cggattcttg caccgatttc gtatttcatc aaatcacctc taccttgatt gtaccacatg
    36961 aattctgtta tgtcaacaaa gaaatcaaga aaatctgtat agcccgcttg acttcacaga
    37021 taatctgtgg taaaatgata ataaatccag aatttctgga aaggagtctg tcttatgaga
    37081 gtttatgaga aagttcgcac atacatggca aacaatgact taaaagcagc aaatgtcgca
    37141 aagaaagcag gaatttccgc taagtctttt gatgcgattc tgaacggaaa acaaacgctg
    37201 tacccggaag acctgagggc gatttgctat gctctgaacg taaagcctga aaagtttatg
    37261 taaacgatca aacgagacaa gagcgaaggg taaaaattat ggtacttgaa tttttgaagt
    37321 atgcgtactt caatatgaca aagggtgata gtatgaatga tattttaatc aaatgtgcag
    37381 aaaaagccta tctggacttg tgcagaacga taaagttcaa cacagagaat aaagacacta
    37441 gaaaaagtgc aaagcgaaaa atctgtgaaa tgctcgtcca tgaatatgac gttttggaga
    37501 atgtcgtaaa gggttctgat gaaagacaga gtgcctttga ccgtgagcat caaagaatct
    37561 gcgaagagat catcaatacg tattcggaaa tcagcgaatt gacgtatgga caggcacaaa
    37621 agtggctgaa tatgatgcta aaatatgttt tgatgacggc ggaggactct gctctgaaaa
    37681 actatctgca cattcctgtg gatagctata ttatgcaggc tgttggctca gataattcaa
    37741 agctgaaaca ttgtctgaaa ctggaatgcg ttccgaaaaa ggacggtact gtaggcaagt
    37801 atagcgaaag cacttcaaag ccctggagca agtggaacta tgaggaatat attgctttcc
    37861 aaaattgtat cagaaaagca gtttccgaaa gtgattataa ttccccgatt gaatgggaaa
    37921 atgcggcttg gatcgaggtt gcagaatata ggaaataaag gagagaatta gaatggcttt
    37981 ttgtacgcaa tgcggaagca aattaaatga cggtgctgtt ttttgcggtc aatgtggtgc
    38041 taaagtattt tctggcacag cacaatcgca atcgacaaat aatggctttt ttaacaagct
    38101 gaacagtact gtccaaaagg ctgtgaacgg tgcgcagcaa gtagttggtg acctttcggg
    38161 aagccaatca gctaatagtg cttcaaataa tacatctcac actatgtatg aagcgtttgc
    38221 cccaaaattt ggcataggta gaaaatttat tttcaccgag aattctttga tttatgggga
    38281 taatgaatac aaatattctg aattgacgca gattttttta gttaacccac ctatgcaatt
    38341 tacaaatggt gtggctaata caatggcaaa tggcaaacgt ctgatattgg cttttgagta
    38401 tagtcagaaa gaacgctttg caagagcatt gacatatgca aatgagaaga ttgcttctgc
    38461 aaatggaacc cctataaact ataaatatat tttgcagtca cccgatggga taaagctgga
    38521 aatttatgag gattatgcga ttctgtatta tctcgaatct ggacacagga atattttgag
    38581 caattcaatg cagggaggtt caagcggaag tattatatac ttctccaacc tgacgttaca
    38641 actgataagc actgtggata atacatctgc tttacagata gatatagtga acaataatag
    38701 cacatctacc atttccatgc ccttaaatcc gcaagatgca gatactgcaa aatctgctat
    38761 ttcttatatc gggaaaataa aaaacagtga acagtataaa tcacaggaaa cagtaccaga
    38821 tgactgggaa caagtagttg gttcggtcag gaagttttct gtatgcggaa aagagcttga
    38881 aatatcggag agtatggata tttttaattc atatagactg aaattccgtg agcttgcttc
    38941 tgcctgtact gataatgcaa gaaaagaata tgacaaaaaa gttcagaacc tctctacatt
    39001 tcttggattt tatccccaaa tatatcgatc ccatctcaat attctgatca aacagtccat
    39061 gaacatctta atttctgagg gtatatggac agttacagaa gattcatttg aacaacagca
    39121 ccttactaat ttccacctcg gaatgaatga ctatacctca acgcttgaaa gtattgaact
    39181 gacactgcag tcaaaccgta gaataatgtc aaatataacc agcctcattc caaatctgag
    39241 tggcggtggt ttgggattaa aaggtgcagc gaaaggtatc gcaacagcta cagcctttaa
    39301 tattgtacgt gacggagcag aagcaggact tttgaatagc gtatctaacc tcaatcaggc
    39361 tcagcaagca gaattatatg gtcgcattaa gcctgacaat ttgtttgact gtgtattcac
    39421 ggattattgg cgagtgttct tatcacttgt atgcttatta aataagaacg gaaaaactat
    39481 ctggtatccc acaaatacaa caacgcaaca ggcaagcaat atatttacca atatgtcaaa
    39541 tccgaatttt ccacaagaca agttgatcga tgtatttctg gatattctta aaatcaatcc
    39601 atacaatgca gaatatcaca aatttatgat atctcgcttt ggcgaaaatg atgaaacaac
    39661 agccattaaa aattatttcg gttataccga ttttgataat ccaagaatca cataatataa
    39721 aaacaagcat ctcttttcgt ttataaaatt tcatacaaac aaaacagccg taatcgtaaa
    39781 aaattacggc tgttttttat aaaagaaaca gtattatttt ataatgaggt attttcgatg
    39841 atttacggat atgcgagatg ttccacagaa ccagagaaca taaaattctc tggttctttc
    39901 gttttgctat tacagccaaa ccgtaatggc aaagccacct acaaagtaat agggtttgtg
    39961 taatattgtt ctggtggagc ataggggagt cgaacccctg gccttcacaa tgcgaatgtg
    40021 acgcactacc aactgcgcta atgccccgta tgcttttatt atagcaccgg aactgcgctg
    40081 tgtcaagcgt gtttttgcag gcataaaaat aaccgcccca cttccacata aggcggctat
    40141 tttaacaaaa acgcttattg ggagcaaacc gtctaccggt atgctctttc tatttctatt
    40201 atagacgatt tcttttgctt tgtcaagcgg ctgtccggat tttttacaga aaaatccctt
    40261 gacaactgcc gggcaatatg gtatgataaa cacattatga aatgcgtgga acaggagaag
    40321 tatctgcatc tgtgccggca cagagagact tcgggatggt ggaacggagg agcggcgccg
    40381 cagagaatac acctgggagc aggttttctg aaacttttgt gcgtagggag atccgggtac
    40441 gcccgttaca gcgtctttga ggtcggacag gcggctgcct ttccgataaa ttgaggtggt
    40501 accacggtga aaacatcgtc ctctggaaaa atcgtttccg gagggctttt ctatacctcc
    40561 ggggaaagag ggaaaaacat gacagtattt gaagaactga aacgacgggg tctcatcgcc
    40621 cagatgaccg acgagaaaga gatcgaagaa ctggtgaacg ccggaaaggc aaccttctac
    40681 atcggttttg accccaccgc agacagcctg cacgttggcc attttatggc tctgtgcttg
    40741 atgaaacgtc tccagatggc aggcaacaag cccattgtcc tcatcggcgg cggcacagca
    40801 atgatcggtg acccttccgg caagaccgat atgcgaaaga tgatgaccaa ggagaccatc
    40861 cagcacaatg tggactgttt caaaaagcag atgagccgtt tcatcgactt ctcggaagac
    40921 aaggcaatca tcgtaaacaa cggcgactgg ctgctggatc tgaactatgt ggatgttctc
    40981 cgggatgtag gcgcacattt cagcgtcaac cggatgcttt ctcacgagtg ttacaagcag
    41041 cgcatggagc gtggtctgac gttcctggaa ttcaactata tgatcatgca gagctatgac
    41101 ttctataagc tgttccagga ttacggctgc aatatgcaat tcggcggcga tgaccagtgg
    41161 gcaaatatgc tgggcggcac ggaactgatt cgcagaaagc tgggcaagga tgcctatgct
    41221 atgaccatca ccctgctgct gaactccgag ggcaagaaga tgggcaagac cgaaaagggc
    41281 gctgtatggc tggatgccga aaagaccacg ccgtatgagt tctaccagta ctggcggaat
    41341 gtctccgacc aggatgttat caagtgcttg aaacttttga cattcgttcc cattgagcag
    41401 atcgaagaga tggagcgcac cctggagggt cagcagctga accaggcaaa ggaactgttg
    41461 gcatacgagc tgaccgaact ggtacacggc aaggaagagg cagacaaggc acaggctgcg
    41521 gcaaaggctg tctttggcgg cggcggaaac aacgccaata tgccgacaac cgtgctgaaa
    41581 gcagaggaac tgacagacgg cggaatcggc attctgacgc tgatggtgcg ctgcggactg
    41641 tccccgtcca acggtgaggc tcgccggctg gtacagcagg gcggcgtttc tgtgaacgac
    41701 accaagatca ccgacccgaa gagcgtgatc tatccggaag cagaaaccat tctgcgaaag
    41761 ggcaagaaag tgttccataa ggtggttgtg gaagaataac ttcgaagggg tcccctgaaa
    41821 tagaaatcaa aagggcgttc tgtgcttgca ggatgctctt tttattgcag ggaaatcaac
    41881 gcagaaatac aaataaaatg ttgacatctt ttatgttgta cgttatcata aaaatatcct
    41941 gttttgcatc tgtcgcacaa tctttgtgcg gtattgccct aagaatgata tggaggaaga
    42001 aaaatgaaat tgtggaaaaa agggttggcg gcattgactg ctggcttgct ttgtctgggc
    42061 agtgtgggac tgtctggcgt ggaactgccg gcgagtgcag atgtgccata tttttacgac
    42121 ggtacttatg gagatttgta ttatgacgtg atcgatgcag tcgaaattcg aattactggt
    42181 tgtgaaaaag aagttacggc tgtagagatt ccagcgaaga tcgctggaaa accggtgacg
    42241 agtgttggaa gaagtgcttt ttctggctgt aacagtctgg ctacggtcac gataccggac
    42301 agcgtaacga gaattggctt agatgccttt tataagtgca gcagtctgac cacgatcacg
    42361 atgccggacg gtgtgacgat tcttggagcg gacgcttttt ctttctgtac cagtctgacc
    42421 gaagtcacga tgccgaacag cttgacaagt attggaagta atgccttttc cggctgtagc
    42481 agcctgactg aaatcgagat accagacagc gtgaccagca taggagagag tgctttttcc
    42541 gactgtaaaa agctgacctc gatcacgata ccggacagcg tgacaagcat tgaaaaatct
    42601 gccttttccg gctgcaacaa cctcactatt tatggttacg cccgctctta cgcccagaaa
    42661 tacgcagccg aaaacaacat cagatttgct ctgatcggcg gattgctcag gggtgacgta
    42721 gacggcagcg gcggcattga ctccaccgac atcttttaca ccatgctcta catcgccaac
    42781 gttgccgtag gcaatgacgg cggtctgacc ctggaacaga tcgcagccgc cgatgtggac
    42841 ggcagcggca aggtggactc cacggacgtg ttctatatga tgtactacgt ggcgctccac
    42901 ggcgtcggca aggatgtgtc ctgggaggaa gttctcgcta agtaagcgta aaattcttga
    42961 tattccaaaa ggcgttctgc gtttgcagga cgcttttttg tgtgcgggaa agaagctgcc
    43021 ccgaaaaggg aagatgccat gaagtgacag aggttttttc tccttggaac acagattgat
    43081 ttgcgtggaa aatgcggata aattgttgac tattgtacct gatatatgtt ataataacga
    43141 tattctattt tgtgaaaacg acatggagga gcaaaaatgc agttatggaa aaaatcgctt
    43201 gcggcactca cagccggcgt actctgtctg ggccgtatag gcgtgtccgg ttcgctgaat
    43261 atgccggaga gcgtgggcgt attgtcggca agtgcagaag aaggtactta tggaaacctg
    43321 tcctatgatg tgacaaatgc aggtgaaatt gaaattaccg gatgtgatat gaatgttgca
    43381 tccgttgaaa ttccggcgga gattgacggg aaacgtgtaa cgagtattgg agacaatgct
    43441 tttcgtgact gtaccagtct gacagagatc acgataccgg acagcgtgac aagtattgga
    43501 gacggtactt tttacggctg tactggtttg accgaaatca caataccgga cggcgcaaca
    43561 agtgttggat ttcagacctt ttccggctgt accagcttaa agaaaatcac cataccagac
    43621 agcgtaacga gtattgaaaa taacgctttt tatggctgtg ccagcttgac ggaaatcgtc
    43681 attccagacg gtgtgacaaa aatttacagc ggtgcttttt acaaatgtac cagtctgacc
    43741 gaaatcacca taccggacag tgtaacgaat atcagagtgg gtgctttttg cggctgtacc
    43801 agtttgaaga aaattgttat tccggatggt ataaccagta ttgaaggcag cgttttttac
    43861 ggttgtacca gtttgacgga aattaccatt ccagatagtg taacgagtat ttggagcagc
    43921 gcttttcgag gctgttccag cttgacggaa attacgattc cggatcgtgt gacgagtatt
    43981 ggcgacagtg ctttttacag ctgtaccagc ttgaccgaaa ttaccattcc agacagcgtg
    44041 acgaacatcg aaggatttgt ttttacggat acgccgtggc ttacagccaa gcaagaggaa
    44101 aatccgcttg ttatcctcaa tggaactttg attgatggaa cgacttgtac cgggagcgtt
    44161 acgataccag acggcgtgac aagcattgca ggcggcgctt ttgacagctg taccggtctg
    44221 acggcaattg ccattccgaa aagtgtaaca agcattggcg acagcgcttt ttacagatgc
    44281 accagcttga cagagatcac cattccggac agcgtaacaa gcatgggcga ttatgttttt
    44341 gacggctgta ccggcttgac gaaaatcacc attccggaca gcataacaag tattagcgat
    44401 tatgcttttc gcagctgtac cggcttgacg gaagtcacca ttccggacag cgtaaccagt
    44461 attggcgatt atgcttttcg tgactctacc ggcttgacga aaattgtcat tccggacagc
    44521 gtgacaagta ttggagacag tgcttttgat aactgcgata atctgattat ttatggtcat
    44581 accggttctt ttgccgaaac ttacgcaaag gaacacggga ttcaatttgc agcttatact
    44641 tgcggtgacc tggacaacag cggaaaagtc gactccaccg acattttcta caccatgtat
    44701 tacgtggcaa atgttgccgt aggaaatccc ggcggtctga cacaggaaca gatcgcagcc
    44761 gccgatgtag acggcagcgg caaggtggac tccacggacg tattctatat gatgtattac
    44821 gtggcactcc acggcgtcgg caaggatgtg tcctgggagg aagtcctcgc taaataagcg
    44881 taaagttttt gatatttcaa aaggcgttct gcgtttgccg cagggcgctt ttttgccact
    44941 ccacgcagcg taggttgcca agcggcgaaa actctgttat actggataca gtcgatcgac
    45001 gcatgacaaa ccaaaggagg aaacgcttat gaatccctat gtgaccggac agaccatcca
    45061 gatgctccgg gaaaaacgcc gcatgacgca gaaacagctg gcggatctgc tgtatatcag
    45121 tgacaagacc gtttccaagt gggagacggg aaaagggctg ccggatatta tgctgctgga
    45181 gccgctggca aaggcgctgc acgtctccgt gccggagctg ttttccggga cagagatcgt
    45241 gaaccagaac cgcaccggca atatgctgaa aacccggttt tacgtctgcc cggtttgcgg
    45301 caatgtgatc tctgctgccg gcgagggcgc tttccactgc tgcggcattc tgctgccgcc
    45361 gctggaaccc gaagaaccgg acacggcgca tgacatcatc gttacgccta tggacgggga
    45421 gtattacgtc cgcatggcgc atcccatgac aaagcgccat ttcatttcgt ttttcgccgc
    45481 agtgactgcg gaacgggtga ccctggtgaa actctatccg gagcaggaac cggaaacacg
    45541 tctcccggca aagggcaggg gctggctgta tgcgtactgc aaccagcacg gactgtttcg
    45601 cctgccgctc cgccgggaac agcgctgaat gggcatacaa aacaaaaaaa gcacttccgc
    45661 agcgaaatgc cgcaggagtg cttttgcttt atgcaggaca aagtgttgga aactcagtcc
    45721 tctttttcgg ttccgtcggt atcgctgcct tcgtcagcct ccggcatatg gagctgtgcc
    45781 ggctggtctg cggcatcgtc atccgaaccg aaggtgcaga caccatctgc acagcagccg
    45841 gatgcagcgg cagcgtcctc tgtggtttcc tcgccgtcct ccgcagtctc ttctgcgtcg
    45901 gcagtttctt ccggtgtgtc gcagagacag atatcgtcat cttcgtcctc gtctgtgcag
    45961 cagcatccgc cttccggcag gtcgtcacag ttgcaggcct tgtccagtct cttgtcgtgt
    46021 gcttttttca gtgcaaatac agttgcagca gaggctgcag ctgcggctgc gatcattgca
    46081 gcaatgatgg ttcctttcat gtcgatacct cttttctccg acaccttgtg tctgttgtga
    46141 tctggaagtt ctgcgggtcg gcggcagaaa acacagatgt ggtttacatt cttattatac
    46201 catgttttca aagaaaatac aagccccgac atccggaatt ttggcgttta tgcggcaaaa
    46261 attttcagag tggcgaagat cagacctgtt ccagcgcctg tttcaggtcg gcggtgatat
    46321 cgccgatgtt tnnnnnnnnn nccagctgga gcgcatccat aaaccggacg gctgcctctc
    46381 tgccgccttt gatggagatg gagataacgc cgcaggtgcc gtccggcaaa tacttttctg
    46441 ccagcttgtg gtacgggtcg tccggcagac cgggataggt caccgatgcg atcttgtcgg
    46501 actctttcag catttttgcc acagtcatgg cgttttcgca gtactgcttc atccgtaccg
    46561 gcagagtctc cagtcccagg ttcagcagga atgcggagtg tgccgccgga tagcagccga
    46621 agtcacgcat cagctgcatt ctcgccttga tgatgtatgc cattttgccg aactcccggg
    46681 tgtagaccac gccgtgatag ctttcgtccg gctcagtgaa atccgggaac ttgccgctct
    46741 tttcccagtc gaatgtgccg ccgtccacga tcacgccgcc gcactggagc gcatggccgt
    46801 ccatgtactt ggtggtggag tggatgacga tatccgcacc gaactcgatg ggacggcaga
    46861 ggatcggggt agcaaaggtg ttgtccacga tcagcgggat ctggtgcgca tgggcgatct
    46921 tggcgaactt ttcgatgtcg aacacggtca gcgccggatt ggcgatggtc tcaccgaaga
    46981 ttagcttggt gttgggctgg atcgcctggg aaattgtctc ctcatctgcc aggggatcga
    47041 cccagatgca ctcgatgccg aaccgtttca gcgtaactcc gaacaggttc actgtgccgc
    47101 cgtagatggt agagacggaa acaatgctgt cgccggcgct gcacagattc agcacggaca
    47161 gcagagacgc tgcctgtccg ctggaggtga gcattgcgcc cacgccgcct tccagggctg
    47221 cgatcttctg ctcgacagcg tcacaggtgg ggttggcgaa acgggagtac atgggttcca
    47281 tgggcatatc gaacagctct gccacctttt cggtgctgtc aaagcgatag gtggtactct
    47341 gtacgatggg gacagttccc ggaccgccgt ttttcggggt atagccggcg tgtaaacatt
    47401 tggtttccag tttagcgttc ggattgcatg gctgcatgat aaattcctcc gttgtgttgt
    47461 atttttatga taaaaaagat gatattatta taccacattt tgtttcgtct gcatagatgg
    47521 caattttcag ctctatccca taggaaaaat ctatggctgc cgaaacagcg gcgaatccgg
    47581 cggaaacctg ctataattgc cgaatttcag aagaatgggt catgcagaat tggcaaaatt
    47641 gcaaattttc cggaaatgcc attgacattg tgtgtggaaa atcgtataat aaaactatgt
    47701 attgttgtgc gtgaagaagg aatccgggag cataccggcg ggaccgcaga aagcggtggt
    47761 gtgcgtcaga ttccggacaa atcagaagct ccgagcaacc acgggccttc tgcatgaaat
    47821 acttttttga gcagtttctg caaagtacgg gaaaactgga tttttttgca gatgctctgc
    47881 tgcctgtctg acggacgtga attgtcagca ggccgtatct attgatttat attattatat
    47941 gtgaccggaa ttcagaaaac gccgggcgaa acctgcacac aagctgcgtg cagcgcccac
    48001 gggcgtgcct tttgaaattc ccgtcagaat ccgagaaagc tgtacggcgt aaaaggcagc
    48061 tttccgggga cagccatcgg gcagaggaaa aatgcaccgc cgctccggca ggtgtgacag
    48121 gaatccggga aagggtgtga ccctgtccgg acgttatttc gagcgtgttc cgagaaatgc
    48181 ttgtgtattt ttatgggaac cggtctgcgt gccttttccg gcagaacgag agacaacggc
    48241 ttggaaaagg acaggatcaa aatacaattt catccatgcc agacagaaca tccggcatag
    48301 cacggatgcg tcttggtgat cgtgctgtcc ccacgtgaga aagtgaggat atttatttgg
    48361 ctaacgaaac agaacaggta agagaagtaa gaaataatcc gcagccccgc aagcggaatc
    48421 acggcgcaag accccggccg cagaacaacg gcggcgagcg tcagtccggc ggaaaccgcc
    48481 gctacagcag ccgtcccaga aacaacaacg gcacacagca gtcccgccag tcccggcaga
    48541 aacgcacccc ggtgcgcatc attcccctgg gcggcctcaa cgagatcggc aagaatatga
    48601 ccgtattcga gtgctgcaac gatatgttta tcctggactg cggtctggca ttcccggata
    48661 tggatatgcc tggtgtagac atggtcatcc cggacttcac ctatgtggag ggcaacaagg
    48721 ataagatccg tggtatcgtt atcacccacg gccacgagga tcacatcggc ggtctggcat
    48781 accttttgaa aaagatcaac gtgccgatct acggcacacg catgaccctg ggtctgatcg
    48841 agggcaagct ggaggaacac ggcttgctga aacaggcaaa actgatcgag atcgaaccac
    48901 gcaccaccgt aaagatgggc tgcatggcgg tggaattcat taaggtgaac cactccatcc
    48961 ccggtgcagt gggcatggca atccatacac cggcaggcat catcgtccat accggtgact
    49021 ttaaggtaga cttttcgccc attgacgacg agatcatcga cctggcacgg ttcggcgagc
    49081 tgggcagccg tggggtgctg gcgctgatgg cagactccac nnnnnnnnnn nnnnnngagc
    49141 gcaccggttt cacttgttcc gagcgcaccg taggcggcag ctttgaaaag ctgttcaagc
    49201 gtgcagaggg acggcgcatc attattgcta cgttttcttc caatgtacac cgggtgcagc
    49261 agatcatcga ccaggcgaaa aactacggca gaaaggttgc cgtattcgga cgcagcatgg
    49321 ttcatgtcat tgaaaaggcg acggaactgg gctatctgaa agtaccgaag ggaatgctca
    49381 tcgacatcga cgaaatgagc cgctatccgg acgaaaagat cgtgctggtc actaccggca
    49441 gccagggtga accgctttcg gcgctgaccc gtatggcaat gaacagccac agaaaggtga
    49501 ctatcacacc caacgacttt atcatcattt cggcaaaacc cattccgggc aacgaaaaat
    49561 ttgtgacccg tgtggtcaat gagctgatga agtccggcgc tgaggtcatt tacgaatcca
    49621 tgtatgaggt acacgtctcc ggtcacgcct gccaggaaga gctgaaactc atccaggcgc
    49681 tgacgaaacc gaagttcttt atcccggttc acggagaata taagcacctc atcaagcacg
    49741 ctgagattgc aaagtccatg ggcatggcag acgaccacat tatcattgcc agcatcggcg
    49801 atgtgatcga aacggacggc aattccatgc gcattgtttc ccaggtgccg gcaggccgtg
    49861 ttatggtaga cggtctgggt gtgggcgatg tcggttctat cgtgctgcgt gaccgtaagc
    49921 atctggcaca ggacggcttg attattgtgg tgatcggcat tgagcgtgcg gcaaatgctg
    49981 tggttgccgg cccggatatc atctcccgtg gctttgttta tgtccgggaa tcggaagaac
    50041 tgatggacga ggcacgcagt gtgctgaccc gtgtgctgga caactgcaat gcccgtgatt
    50101 tgaaagaatg gggaacgctg aaaaccaagc tgcgtgatgt tctgtccgac tttatctatg
    50161 aaaagaccaa gcgcagtccg atgatcctgc cgatcatcat ggaagtataa ttgcctgtca
    50221 aagcaagaaa atcattttga tttttgttgt gtaaaaggct tttgagggca gccgcctccg
    50281 aaaaaacggc tgtctgcaga ggagaataca gcatgaaacg aaagtatggg gtaatgatcg
    50341 ccacgtggat cctgtggttc atcagcattc ttgtgccgct cttctgtgtg gtgctgaaac
    50401 gagtgccgca gcggtttctg accgtgcagt ttctggtgat cctgctgctg ggaatcctgt
    50461 tgtgtgtcca gtggatctgg ctgcgccggg cagaacggct gcaaaatacg gcgatccgtg
    50521 gcgagatcga gggcgcaggc atggcagcgc tgcaaagcat gaccgttcct gtgatcctgg
    50581 cgatgcgcac cggcgagatc ttgtggtgca atgagatatt ccagtcccgt tttgcggcga
    50641 gccgctctgt ccagggacgc tccgtccagg acgtgatcca tctgaatctg gcggcgctgg
    50701 atcagaaccg ggacctgctg atggaacata acgggctgag ctatcgggta cacgccgtgt
    50761 ccagcgtccg ggatcagcag gagatcattg cggtgcagtt cctggagatc accgatattc
    50821 tgcgtttgca gcatgaacag accaattccc gtccctgtgt gctgctgctg gtcatcgaca
    50881 gctatgacga tctgctgcag tatgccagag agagcgaaaa ggcacaggtc tccgccgagg
    50941 tggagcgtgt gttggaggac tttatgcagg ggacagacgg cgttatccgt aaggtggaga
    51001 acgatttgtt ctacgccgtg atggagtccc ggcatctgca cgagatcatg aacaaccgat
    51061 tccacgtgct ggacgaggct cgccagatac gggtcaacga ccggatgcac attacctttt
    51121 ccatcggcgt cggcgacggt gcctctacgc tggcggaaag cgaaaagttt gcccgtcagt
    51181 cgctggatat ggcactggga cgaggcggcg accaggcggc agtcaagacg gaaaacggat
    51241 tctgtttctt tggcggcgca tccaagggcg tggagaaaaa gtccaagacc aagatacgct
    51301 ccattgccct cgccatgcag gaactcatcg agaattccga ccaggtattt ctgatgggac
    51361 accgtttcgg agacctggac gccatcggtt ctgcctgcgg tctcgccggt gcggtgcggc
    51421 tcatgcagaa accggcgtat gtggtggtga accggcagag ctgtcttgcc acccagctga
    51481 ttgaccggat gcagcagtgt ccggacggtc cccagtttat cgagccggtg gatgccctgg
    51541 cgcaggtcac cgacaacagc ctgctgatta tcgtggatac ccacaacaag gacatcctgg
    51601 aaagtgtnnn nnnnnnnatg cggctcggta tgtcgtcgtc atcgaccacc accggaaaaa
    51661 cgtgaatttc atcgaaaatg cggtgatttt ccaccacgaa ccctatgcgt cctccgcatc
    51721 ggaaatggtg acggagatca tacagtattt ccggctggat acggagatct ctgcggcata
    51781 tgccgacgca cttcttgccg ggatcatgct ggacaccaag aactttgtca tgcgcacggg
    51841 tgtgcggaca ttcgaggcgg cggcatattt gcggaagatc ggcgcagata ccatccaggt
    51901 caaaacgctg ttttccaata ccatttccat gtactccatg cgggcgcaga tcgtggcgca
    51961 cgccgaactg taccggaaca acgccatttc tgcggtcaag ctccaggaca aaaacgtccg
    52021 tgtgctggca tcccaggcgg cagatgaact gctgagcatc cagggcgtgg aggcgtcctt
    52081 tgtgctgtat ctggaggaaa acggcgtggg catttccgcc cgttccatgg gcaatgtcaa
    52141 cgtgcaggtc atcatggaac agctgggcgg cggtggtcac cagaccatgg cggcaacgca
    52201 gctcaaaggc tgtaccattc aggaggcaaa ggaacagctg ctgctgattc tggaggcgca
    52261 ggaaaatgag aattagggca gtacagtttt tcttaggaat ccatttctgc tgattacagc
    52321 gaaattacaa t
//
LOCUS       DAWENO010000002        39762 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_412_length_39762_cov_11.425693, whole genome shotgun sequence.
ACCESSION   DAWENO010000002 DAWENO010000000
VERSION     DAWENO010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 39762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 39762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39762
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_39762_cov_11.425693"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2810..2819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7327..7336
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7747..7756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12974..12983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15686..15695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18298..18307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9595 a         9726 c        11107 g         9274 t
ORIGIN      
        1 acggtcagac cgtggttctg ccggcagagg aggtcactgt aggcgacctg gttctcctgg
       61 aggctggcga ccagatcccg gcagacggca gactggtgga ctgcgcatcg ctccagacaa
      121 acgagagcgc actcaccggc gaaagcacca acgtggagaa aacggcggac tgcatcaccg
      181 gagaagtgcc gctggcagag cggaagaaca tggtttattc cggcggcttt gtcacctacg
      241 gcagaggtgt ctgtcttgtg accgaagtgg gaatgcagac cgaagtggga aagatcgcat
      301 cgctgatcca gaatgcagag gctcggaaaa caccgctgca aatgacgctg gatcagttcg
      361 gcaagcggct ttccatcggc atcatgatcg tctgtgcgct ggtgtttgtg ctgagcatga
      421 ttcgtgtgga tgtcatcaat ttcgatgcag tcatggattc tctgatgttc gccattgccc
      481 tggctgtggc agcgatcccg gaggcgctca gttctatcgt caccatcgtg ctttccttcg
      541 gcacccagaa aatgtcccag gaacacgcca ttatgcgaaa gctccaggcg gtagagggac
      601 tcggctctgt ttcggtcatt tgctcggata agaccggtac actgactcag aacaaaatga
      661 ccgtccggaa aatcgtggta caggatcaca tgattgctgc gaatgatgtc agcccggaga
      721 atccggagga acaggaactg attctggcat ccgttttgtg cagcgatgcc acctgtcaga
      781 acggcacaga gatcggcgac cccacagaga ctgctctggt gcgctttgcc caggattgcg
      841 gtctctcgga tgaagagatg cgcagcagcc atgctcgtat cagcgaaatc ccgtttgatt
      901 ccgaccgtaa gctgatgtct accctcaacc agtgcggtga cgtgcggaag atgtatacca
      961 agggtgcaac cgatgtgctg ctggaccgga tgctggtctc cgatgcagaa aagcaggaga
     1021 tcgtcaagca ggtggaggaa ctttcagaac agggactccg tgtgctgtgc tttgcgtcca
     1081 agacgttttc cggcgacgtg ctggatgtgg cagatgaaaa cggtctgaac tatctgggac
     1141 tgatcgctga aatggacccg cctcgtgaag agtccaaaca ggcggttgcc gagtgtaagt
     1201 ctgctggcat caagccggta atgattaccg gcgaccacat tgtcacagcg tctgcgattg
     1261 ccaaggaaat cggcattctg gaaaaaccgg aagaggcggt agaaggtgcg gcactggacg
     1321 cttacaccga cgaacagctg gtggactttg tggcagataa atccgtctat gcccgtgtaa
     1381 cgccggagca taagatccgc attgttcggg catggcagaa acgtggcaat atcgttgcga
     1441 tgaccggtga cggcgtcaac gatgccccgg cgctgaaaca ggcagacgtg ggcgttgcca
     1501 tgggtatcac cggtacagag gttgccaagg atgcggcagc gatggtactg acggacgata
     1561 actttgcgac catcgtcaag gcagtcaaga acggcagaaa catctacgag aacatcaaga
     1621 aagcaatcct gttcctgctg tccggcaatc tggcaggtat cattgccgtg ttgttttgct
     1681 cgctggcagg gctgcctgtg ccgtttgcgc caatccacct gttgtttatc aacctgctta
     1741 ccgactcgct cccggcaatt gcactgggac tggaacccca ttccgatgac gtgatgaaac
     1801 ggaaaccccg tccggcaaac gaatccattc tgaccaagga cttcctcgtt aaggtcggct
     1861 attccggcgt cattatcagt atcgccacca tcggtgcatt cctgctgggc tacttctacc
     1921 agggcgcaca caatgccggc gcagccatga ccatggcgtt taccacactg tgtatgtccc
     1981 gcctgttcca cggattcagc tgtaaggcgg agaaaccggt gatcttctcc aagcgtctgt
     2041 ggaataaccc gtttgccatt ctggcatttt tggcaggcat ggtactgatt aacgctgtgc
     2101 tgtggattcc ggggctgcat gagctgttcc agatcgaaaa cggtatgtcc ggcgtactgc
     2161 tgggtgcaat ctatggcttt agctttggct ctatggttct gattcagatc gtgaaagcca
     2221 ttgccgctgc tgtaagaaag aaataagaaa agctgttttt gcagaaaaaa tccctgcact
     2281 gtatatttcg cagtgcaggg attttgtgtt atagactggg ttcgtcgatt gctttcagaa
     2341 acgtcagact ccgttccaga atcccgttga gaaaagcaat caaaacgccg taattggtgt
     2401 agggaacacc ctgtgcagca gcccgtgcag cacggctttt catttcctgt tggttcagca
     2461 tacagccgcc gcagtgtaca accagttgat acggggacag atcttccgga aagctgttgc
     2521 cggaggaaaa gtcaaactga aagctttttc cggtgtaagc ctgtatccat ttcggcagct
     2581 ttaccgtgcc gatgtcgttg cactggcggt ggtgggtgca accctcagag atgaggatgc
     2641 ggctgccatc cgggagcgtt tgcagcgtct gtgccgcctg cattgccgga gcgagaacgc
     2701 ccttgagcct tgcgaacagg atggaaaacg aagttagcag aatttcttcc ggcacaatgg
     2761 agcgaaccgt tccgaatacc tggctgtcgg tgaccacaag gcgtggaggn nnnnnnnnng
     2821 ctctttcaac agggaaaggg tacggcggag ctgttccggc tgggtcacca cgcagatagc
     2881 ggtctggtct aatatctccc ggatcgcctg cacctggggc aggatcatcc gacctttcgg
     2941 cgcagattcg tcaatgggtg tcaccagaag tactacatct tccggatgca gcaggctgcc
     3001 gaacaggcgg acagattgcc gtgcgatggg cagcttttga taaaacctgc ccagttcctc
     3061 cttgagttcc cggattccct cgccggtggc ggcgctgacc gcaatggcgt tttctgccgg
     3121 gggagtcgcc tggtcgcatt tgttccagac ctgtatggtg ggaatgtgcc gctgttccag
     3181 ctgctgtagg attttcctgt cctccggtgt cacgcctgct acggcatcga tcaccatgac
     3241 ggcaatgtcc gtatgggtca gcgcccgttt tgcctgccgg acacgcagcg aacccagcgt
     3301 tcccacatcg tccagtcccg gtgtgtctat gagaaccact gcgccgatgg gcagcagctc
     3361 catggctttt tccacggtat ccgtggtcgt gccgcagacc ggggagacga ctgataccgt
     3421 ttgtccggcg atggcattga taagactgga ttttccggca ttgcgccgcc cgaaaaagcc
     3481 gatgtgtacc cgattggcac gaggggtctg ttccatatcc tgttctcctt aaaaccggaa
     3541 atctcttgcg ccgtgcagga tttcctgtag gtactgctct gttttggtgc gtactttttc
     3601 gttggggatg gcgagcagct ggcgctgtaa tacttccttg cccagcgctc tggtttccgg
     3661 agaggcatag tcctccagaa attcctgtag ggtgatgagg gcattggggt gacagcagtt
     3721 ctggatctga ccgctcttac atagggacat gaaccggtcg ccggtgcgtc cggcacgata
     3781 acaggcggtg cagaaactgg gaatgtagtc catctccatg agccagcgga cgacctcgtc
     3841 cagagagcgc tggtcgctga catcgaactg tgcggaattg tcgtccgggt tttcaggaat
     3901 atcatagccg cccacgcttg tccgggagcc gccgctgatc tgggaaatgc ccagcggcag
     3961 gagacgttcc cggactgcct ggctttcccg tgtggaaata atcatgccgg tatacggcac
     4021 agaaatgcgg atgagggcgc agatcttggc gaaaacatcg tcactgatgc cgttgtcgaa
     4081 atcgtccggg ttgatatcct ctgccttttt gatgcgggga atactgatgg tgtgcggtcc
     4141 tacgccgtga actgcctcca gatgctctgc gtgcatgagc agtcccacca gctcataggg
     4201 atagttttcc agcccgaaca gcacgcccag ccccacatcg tcaatgccgc ctgccatggc
     4261 acgatccatt gcctcggtgt gatagtcgta gttgtgtttc ggtccggtgg gatgcagctt
     4321 ttcatagctc tctttgtgat aggtctcctg gaacaggata taggtgccga tccctgcctc
     4381 tttcagcata tggtattctt cttctgtggt ggcggcgatg ttcacattga cccggcggat
     4441 cgctccgttt ttgtggtgga tgtgatagat ggtgtcgatg cactccagaa tgtaggagat
     4501 aggattgtgc agcggatctt cgcctgcctc aatggcgagc cgtttgtgtc ccatgtcctg
     4561 caaagcggtg acttcccgga caatgtcctc ctgggtcagc tttttccggg aaatatgctt
     4621 gttcttggca tgatacggac aataggtaca tccgttgacg cagtagttgg acagatacag
     4681 cggcgcaaag atgacgatgc ggctgccgta gaaatccagc ttgatctgcc gtgccagccg
     4741 gttcatctgc tctgtgactg tctcatcctc gcagagcagc agcaccgcag cctcccggtg
     4801 gttcagcccc ttgcgcagcg ccgcctttgc caggatctca gataacagtg ttttgtcgtc
     4861 cttgtgcgct ctggcatatt ccaatgtgtc catgatctct gcatggttga tgaattcttc
     4921 cgcccgaaga gaatgcggat tatacaatat gatcgccgtc ctttgtggtt ttggcagaga
     4981 aaaactgccg gatattctgt tgctttacct gttaatatta taacacctca gatgcacctt
     5041 gtcaattcgg cgacaaaaaa atgcctagtg aaaaaaactt ttccagaaac ctttttgtga
     5101 ctattgacaa attttctgaa agggtgtata atagttctat gaaattcagc ggctgttttg
     5161 tgcaatacat ccgctgtacg tgagagcgga acagataagc tctaagattg gaaaggaaga
     5221 tggaattatg aagaattttt ggaaacgtct gggcagtaca ggcgtagccg cagccctttg
     5281 cctgggcatg gcgcaggctg taccggctgc tgtggtatct gcggcagatc cgctgttctc
     5341 ttcggagtgt gaggatctta ctctggaaaa cgacgcaaag gtggcgacag atgtatacgg
     5401 caaggcatat ccgggttata ccggtgacgg ctttgtctgg gtcaccaatg ccggaactat
     5461 gagctttacc atagacgcac cggagaccgg tatgtatcgt ctgatgacac gctgcatcat
     5521 gtatctgggc gacacttcga acgatattcg tgaggcacag gtaactgtca cacgttcgga
     5581 tgacagcaca gctaccaaga ccgtcaagat cgcacacaca gacgactgga acgacttcaa
     5641 ctggggcgac ctgaaactga ccaagggcga aaataccatc accttcggcg gcggctgggg
     5701 ctactgcctg tatgacaaca tgacgctgac acagactccc aagccggagt atgaaaaggc
     5761 aaccgatacg ccggtagacc cgaaggcaac caaggagacc aaggcgctga tgtcctacct
     5821 caagagcgtt tacggttctc acatcatttc cggacagcag gagatctacg gcggcggcaa
     5881 tgacggcgat tctgaaaagg aattcaacta tatcaaggac aacaccggaa agttgccggc
     5941 gatccgtggc tttgacttta tgaactacaa cccgctgtac ggctgggatg acggtacatc
     6001 ggagcgtgct atcgagtggg caaaggaccg caacggtatt gtcactgcat cgtggcatat
     6061 caatgtcccc attgacttcg acaactatac actgggcgac atcgtagact ggaaggaatg
     6121 cacctataag aactaccagg catcgaacag cacctttaat actgcaaacg cagtgaagga
     6181 aggcaccaag gagtatgaat acttccagga ggcaatgaag gatctggcag aacagctcca
     6241 gaaactccag gatgccaatg tgccgatcat tctccgtccg ctgcatgagg cgcagggtaa
     6301 tgaaggcaac tacggcgacg gtacttcctg gttctggtgg ggtgaccgtg gcgcagaagt
     6361 atacaaggaa ctgtggaaac tgctgtacac cacactgacc gaaaagtata acctccacaa
     6421 catcatctgg gaatacaaca gctataacta tgcaaactcc gatacttggt atccgggcga
     6481 tgactatgta gacatcgtgg cttatgataa gtacaactgt gactataacc gggatgacgg
     6541 tctcagctcc ggcacgccga acctgagtgc gatctctccg atcttcaact atctgtatga
     6601 actgaccaac ggcaagaaga tggttgccat ggctgagaat gactctatcc cgtccgaaga
     6661 gaacatggtg atcgagaatg ccggctggct gtatttctgc ccgtggtacg gcgatcatct
     6721 gatgtccagc cagtacaatg acccggcaca gctgaaagaa atgtacacca gcgactactg
     6781 catcacactg gatgaactgc cggaggatct gtatggcggt gcgagtgtag aacctggcac
     6841 aacgcttacc acaaaggaaa ccagcgagac agtgtcagag accacaactg tcacagagac
     6901 ttctgcaacg gagaccagcg aaaccgaaaa gagcagtgaa actgtttcgg aaacaacgga
     6961 aaccaccaag gaaagtgtaa cgacttcctc tgaggagcag acgaccgaaa ccacagagac
     7021 aacggaatct tccgcaacaa cagcatcggc tactactgca accgaaacgg aaacaacagt
     7081 tgcaacttct accacagaaa gcaagaccag cgaaacatcc ggcagtgtag atccgaaaaa
     7141 tgtactgtac ggcgatgtaa acctggacgg cagagtggat atcaccgatg ccgtactgct
     7201 gaacaaggta gctgccggcg cagtgaccct ggataccgca gaaaagcagg taaatgcaga
     7261 ctgcgatgcc aacggcgaag tggacagcaa ggacgcagtt gttctgctga agttcctggt
     7321 atctctnnnn nnnnnnattt ttgtcatgtg ttttgctgat gcagaaattc ctcttcctgc
     7381 tggcggcgca gggcatattt tcgtttggtg gcgagaccgc cacgaatatg acgttcctgt
     7441 ttgttctgtt ccagaatctc ttttacctgt gtatacagat ccggctggat ctgttccagc
     7501 cgctcgctga catccttatg tacggtgctt ttcgagacac cgaaccattc tgctgccgca
     7561 cgaaccgtgg ctttctgccg gacgatatac tcgccgagaa tcacggcacg tgtttcggac
     7621 tgctctttca tgatacctca ctccaattgt ggttttgcta cagcatatgc ggcggcacag
     7681 gaaatgagaa cggagcaggt tataaaaatg tgatgcagat tttcagaaaa gtttatgaga
     7741 aatcgtnnnn nnnnnnactt atgcagagtg tactctgacc tctatggagt gcatctcaga
     7801 aaacaaagtg atcccggacg gcggatcatg gaaaggacaa tttacatatg gtttgggaac
     7861 tcatcaaatc actgattttc ggtattgtgg agggcatcac cgaatggctg ccgatcagca
     7921 gtaccggaca cttgatcctg ctggacgaat tcttaaagct agacgcatcc gataacttca
     7981 aggaaatgtt cgatgttgtg attcagctgg gcgcaattct ggcggtcgtg gtcatctact
     8041 ggaacaagct ttggcccttt ggcaaacaga acaatccgca ccccatggcg cagaccggat
     8101 tcggcgcttg tctgaaaaag gacattctcc agatgtggtg caaggtcatt gtggcaacag
     8161 tgccggcggc tgtgatcggt ctgctgctgg acgactggag ccaggcgcat ctgtacaacg
     8221 cctggacggt tgccatcatg ctgatcctgt tcggtatcgc ctttatctgg atcgaaaagt
     8281 ggcacaaaaa ccgcacaccg aagatgaaca gcatcgcctc cttaaagtac agcaccgtgc
     8341 tgctgatcgg cgtatttcag ctgattgcag caatatttcc gggaacctcc cgttccggtg
     8401 cgaccattgt cggcgcactg ctgctgggcg tgtccagaac agctgcggca gagttcacct
     8461 ttttcctggc gattccggcg atgttcggcg caagcctgct gaaactggtg aagtgcggac
     8521 tcccggctac cggtatggag actgcacttc tgctgatcgg catgatctcc gcattcctgg
     8581 tatctatcgt cgtcatccgt ttcctgatga gctatatcaa gaaacatgac ttcaccgtat
     8641 tcggctggta tcgtatcgtt ctcggcgttc tggtgctgat cggcggtatt accggattta
     8701 ttggataagt acatattttg cgtaaaaaat ccccgtgtat cctgctagtt ggatacacgg
     8761 ggatttctct tttgggaatc acatcactgc gcagccggag tgttcgtaac tgcggtggtg
     8821 taggtcgtca gtgttgttgc agccgtgcct gtgccgccgg ggacaatggc gtgctgtttc
     8881 agataatcct cccacagaga aagggagttg cccagcaggt ggatgccgtc cgaagtgaga
     8941 gacgcatcca gacagccgtt ggcgtccatg acagcctcgt tgacgttgag gtagtagatg
     9001 ttatcctgcg gattcgccag cgcctccagc gcctggtttc gggcgttgac agttgcgttg
     9061 ttgatcgcac cgcccggatt gtcctgggat gccgcaacca ccagaatgct ttgcagatag
     9121 ataatcgcat tgggctgtgc ctggcgtatc tgtgaaatga cgccggcgta ggtctgcgcc
     9181 cagctttcag tggtgccggt tcccagctcg ttcagtccca gcatgatata gactttgccg
     9241 tactgttttt gcgccagctt ttgcgacagt gtgacgcttt cgccggtgtt cgggttgccg
     9301 gctgcctttt ctgtgacctt gaaaatggac agtcccacgc tggagaaaaa gtccgcattg
     9361 gtcaggctgc cgtacagcgc cagaccttct gtccgggagt cgccgataaa gagggcatcg
     9421 ctaaaatagg aatcgtccac cgtctggaat acagcggcag gcgctgtggt ttccgtgacc
     9481 aggggctgac cggttgcaag ggtcgtttct gccggctgtg ttgtggtttg cgccatcgtt
     9541 gtggcaggat cctcgatttt tttggcattc tgtactaagt cccagatgtt tgcaactgca
     9601 atggagacca caaatgccat aatcaaagga taccgtttga gaaatttcag aaaattcatg
     9661 aaaatgctcc tttcatggca tgggtcagaa ctggaagtac aggaacggat tgttcgtctg
     9721 tgtcatcatc gcacgaattg ccagcacgaa cagaatcagc aaaagcagca tacagaagcc
     9781 gctcttgcgg aactttttgt aatacatcag aggcagcggc gtgctgaaca gaacacaggg
     9841 aaccaggatc ttccaatagt tgtggaatgc ctgcactgca tccctggtgt tggactgtac
     9901 ctcatccgaa gtgaggaacg ggaacagacg ggtaaagtat gtgccgagct ggtgcatatc
     9961 cgtgatggca aagaccatcc aggtgagggg gatgataaag atgatatagg cgtgtttcag
    10021 aatgtgtgtg cgctctagga acttgccgta gacgttcttt tccagtgtca gcagcacgaa
    10081 gaaaatgaga ccccacagga caaagttcca gcttgcgccg tgccacagtc cggtgaatgc
    10141 ccagaccgcc agcatattcc gcatggtttt ccattttcct acccggctgc cgcccagcgg
    10201 aaagtaaatg taattcttga accagcgtcc cagggtgatg tgccatctgc gccagaactc
    10261 gccggctgag ccggacatat acggcagatt aaagttctgc ggcagttcaa atcccagcat
    10321 ttttcccacg ccgattgcca tcatggaata gccggaaaag tcgaagtaga tctgaaagct
    10381 gtaggcgaat gcacccagcc aggcataagg ggtggagata ctatcatacc cgatcacgga
    10441 cagattattc cagagaccgc ccatgggatt cgccaaaagc gttttggcgc tcagacccag
    10501 gacgaaggtc tccatgccct cttccagcag ccggatgtcc acacggcggt tgtgcatctg
    10561 gggttgaatc tcgctgtaca tacagatcgg accggagatc aactggggga acatacacag
    10621 ataaacgccc agatccagca gacttctcgc cgggcgtgtc ttgttttggt acacgtcaat
    10681 gagataggat gtgatctgga acgtgaaaaa gctgatgccc aggggcatga tgatagatgc
    10741 ctttggaata gcaacgtgaa agaccgacag cagggaattc agattgccga tcaggaagtt
    10801 ggtgtatttg aacaccacca aaagcccgaa gttgtagatc agaccgccga tcagatagtg
    10861 ctttgcctgc tgcggatgcc ttgcgtgcga accgctgata agccgggaaa gcccgtagtt
    10921 gatgaccagg gacaacagca ggagcagggt ataccagccg ctgccgatgg cgtagaaaac
    10981 aaggctgccg atgatgagtg tgatgtttcg gtatttctgc ggtgtagcat agtatgccag
    11041 caggaaaatc ggcagaaatt gaaacaagaa ttcaattgtg ctgaaaacca tatatgattc
    11101 ctcaattccc cttcgatatt tttttctgtt ttgcggtgct gctttttatc tattctatgc
    11161 aggcaaacac aaataacaac accccgacaa gcgaggtgtc tcacattata tcataagaaa
    11221 aatctgtcgt tttttcaaga acgatgtcat aacaaatgag ttacaattca atgacaaatc
    11281 cagtttgtcc cagatgcaga acaggcgctt agtccttctt ttccgcagcc ggtgcatagc
    11341 aggtgaaaac attttccacg tggtcggtgt cctttacctt ggtgcaggcg ctgaccagcg
    11401 gaaccacgat cagagaaagc agcattgcaa ttgcgccggc attgatcggg gatgccagat
    11461 tcagcggcag gcttgcgaca gccttggaca gttccgggtt ccagccaaag ccgaacagga
    11521 acatatgtac cagcgtcaga ccgatgccca agataaagct gcaccatact gccggctttg
    11581 tgaccttctt gctgaacaga ccatacatga acggtcccag gaatgcgcct gccagtgcgc
    11641 cccaggaata ggacatcagc gtggagatcg aggcgttctt gttgaacgcc agcagaacgg
    11701 agatcaccag gaatacggcg atcagcacac gcatgataag catttccttt ttgtcggagc
    11761 atttcttcat gttgggcttg atgaggtcaa tggtcagcgt ggagctggag gtcagcacca
    11821 gcgacgacag ggtagacatg gatgccgaca gcaccagaac aacgaccaga ccaatgagga
    11881 tatccggcag tgccgtgtgc agcagagacg ggatcatggc gtcaaaatcc ggcttgccgt
    11941 tggacagggt ggagatcact gtcttgtcgc tgccgtctgc gctcagcgtg cagaacagcc
    12001 ggtcgaaacc gcccagcaga taagaaccgc cggcaacgat cagtgcaaag acggtggaga
    12061 tcacagcgcc ctttttaata tcgttttcgc tcttgatggc atagaacttg tgtaccatct
    12121 ggggcagacc ccaggtgccg agtgatgtca gaaccacaac gcccagcaga ttcagcggat
    12181 ccggtccgaa tacggagttc agcgtgttgg tcttgccgtt gacttcaata tcgcccagtg
    12241 cagccagtgc agcagtcatg ccgccacggt tgtggatcgc acagatcact acggcagcga
    12301 tgcccaccag catgacaatg ccctggataa agtcgttgat cgcagttgcc gcatagccgc
    12361 ccaggatcac gtagatcgcc gtcagcagcg ccataacaat aatgatgatc ttgtatgcgc
    12421 cctcggactc gatctggaac gccatgttaa acagacggga cagaccgttg tacaccgatg
    12481 cggtataggg aatcagaaag ataaagacga tcacagcagc tgcaactttt agtcctttgg
    12541 agttccagcg cttttcgaag tattccggca tggtttttgc gcccagcgac tgcgtcatga
    12601 tgcgggtgcg ccgtcccagg acgatccatg ccagcagact gcccagcagg gcgttgccga
    12661 tgccgaccca tgcggcagaa acgccgtatg tccagccgaa ctgtccggca tagccgatga
    12721 agactacagc ggaaaaataa gatgtgccgt aggcgaatgc ggtgagccat gagccgacgc
    12781 tgtgaccgcc aaggacaaaa tcactgacgg agcgagtctg ccgtgcggtg tagacgccaa
    12841 tgcccatcat gatcgccaca taaatgatga tcatgccaat ggtaattgcg gttgtcatat
    12901 tcctctcctt tgtggcagca ggaacgctgc caaaacataa atttgcggca tctttcaaaa
    12961 atccgaaaat gcgnnnnnnn nnnaatgtcg gtttttgtca accttttttc tgcttttcgg
    13021 gcgaaatgct gtaaaatgat gaaacggctt gagaatttac atattgttca ctggattttt
    13081 tgcagaaaat tccgaaaaag gcttgacaag ccgtcaaatg tatgctatcc tatagttagt
    13141 caatgctaac tcttgaagaa gcaaagatcc ccagctgtgc ggaacatggc tgacgagtcg
    13201 tgaaacgcac agttcttgat gacgagggtc tgcttggctg ccagaaatat cgacgggctg
    13261 cggcgtgtgc cgcaaaagcc caggaacttt gtgaggtgaa attatccaat gagcgttgtg
    13321 attatcggag gaaacgagtg tatgagccgc cagtatgccg atgtgtgtat ggcgcacggc
    13381 tgtacggcaa aggtgttccc gaaagagacc ggacccatcg aaaagaagat cggttctccg
    13441 gatctgatga tcctgtttac ccgtactgta tcccataaga tggtacaggg cgctgtgaag
    13501 gcagcgaaga aaaattccat tcccattgcc agagtgcaga gcagcagtat ttctgcactg
    13561 cgtgcgctgc tgggacagcg tggcatggta gaggcataag tgcttttcgt ctgcggtgag
    13621 cgtgagatgc tgctgcgaaa aagcttttgc atacaagaga ccggcgtttt ggaagagagc
    13681 gccggtcttt ttttgcagct gtgcgtctgt tctggttgtg aagttgcaca aaaattttga
    13741 taaaacgctt gattattctt gacaaagttc cgccgatatg gtatgataaa aatgtacgta
    13801 aacgtaaccg gcattctgtg ttgtgccgga aatgaagaat tggagtgaag acgaaatgaa
    13861 tcggaaaggg aatttgaaaa caaagctggc agcagtattt tctgccggcg tactggctgt
    13921 tacgggaact gcatcggcag tttccgggat gacagtctcg gcacagccgt cagccggtac
    13981 atacggagac gcactgaaac tgtctctgta cttttttgac gcaaaccagt gcggcgcaga
    14041 tgacaacgtg ctgacctggc gtggaaagtg tcatacctat gatgcagaag catcgctgag
    14101 cagtgccaag ggactgtcct ccagctccat ggcagcgatc aaagcggcaa acggcggttc
    14161 ggatacggta gatgtgtccg gcggctatca cgatgcagga gaccatttga agttctcgaa
    14221 cacaatggga tattcctgca ccaatctggc atggtcttat tttgaaaacc cgaattccta
    14281 taaggagacc ggaagtgaag accacctgct gtacattctg aaaaaaatgt gcgattactt
    14341 tatgaaagtg acctatctgg atgacagcgg caatgtcatt gcgttctgct atatggtagg
    14401 tgacgaccag gatcacaaca tctggactgc accggaagtg cagacacaga acagaccgac
    14461 atactgggca gatgcgtcca acccgtcagt agatgcgtcc ggtcatatgg cagcagctct
    14521 ggcagcaact tctctggcat tccgggataa gaatgcagat tatgcagatg cctgcctgaa
    14581 gtatgcaaac gcactggaaa agtttacaga aaaatatccg aaagcaacct atgagggaat
    14641 cggtagctac tattcctgcg gcaatatcga agataaggta gcatggagtg acctgtggtg
    14701 tgcgatcgcc aacaacaacg gaaaactgcc ggattcctac caggcacagt atacaccgtc
    14761 caacggcgta tacaacggca gtatctacga ttactgggtt tattcctggg ataaggtatg
    14821 gggcggttat tctgctctgc tgtactccat ggatcctcag aagtacagtg cgcacggcag
    14881 cgagctggtg tttgacatgg atcagctggt aggcaacaag aaccaggcat attatccggt
    14941 cggcggcggc tggggtgcat cccgttacaa ctgcgcatgg cagatgtatg ctttgactta
    15001 tgcaaagtat accagcggac aggacaaata caatgaatat gcgcagggtc agatggacta
    15061 cctgctggga aacaatccgg caaaccgcag ctatctaatc ggctttggcg attcttatcc
    15121 gcagcatatc caccacagag cagcaaatcc ggataagggt actgcgaaat acactctgta
    15181 tggtacactg gtcggcggtc cgacagatgc caacggttct tatgacgata acacggactc
    15241 ttattcctgc acagaaccgg cgctggatta caatggctgc tttgcactgg caattgccgg
    15301 actgtatgcc gtttacggcg gttcgacaac agctgcacag agtgcaattg catctgcttc
    15361 tgaaatcaac agcgactttg tattcagcta tggcggcggc gagactcctc agcccggcac
    15421 aaccgagacc acggagcaga ccacgaccac aacagaagag acaacaacga cagcagaaac
    15481 taccacaaca gaggacacta ccgagacacc ggatccgatg gcagcgtggg aaaaggtacc
    15541 ggattctatg atcgtcggcg agaccgctga ggcatctgtt acctattcca tcatcagcaa
    15601 cgacttcgat tgggcatcct ctgacccgga tattctgagc gtagaaggaa acggcaacaa
    15661 ggcaacgctg acggcaaaac agccgnnnnn nnnnncgggt gttattgcgc tgactgcatc
    15721 caacggcttt gaaacgctgc tcaaggaaat taccgtatat ccggcagaaa caactgcaac
    15781 aacggtagag accaccacag aaacaaccga aaccactacc gtttcgaatg aaaccgaaac
    15841 cacagtttct gaaagtgata ccacagcaac ttcttctgaa accacgacag agaccacaaa
    15901 caccacagct accggcgctc cggctggcac actctacggt gacatcaatc tggacggcag
    15961 agtagacatc accgatgccg tactgctgaa caaggtcgct gccggcgcag tgaccctgga
    16021 taccccggaa aaacaggcaa acgcagactg cgatgccaac ggcgaagtgg acggcaagga
    16081 cgcagttgtc ctgctgaagt tcctggtatc tctcatcaag accctgccca gcgcagaata
    16141 acgcacctga cggaacagga ggtttgtgtc tcatgaatcg aaaagccctg caaaagctgt
    16201 cttacggggt gtacctggtg acgacagcgc agaacggaaa atcctatggc tgtgtggcga
    16261 acagcgccat gcagatcacc tcttctccgg cacagatcgc cgtgagcatc aaccatgaca
    16321 atgccaccca cgaccagatc gcacagagcg gaaagtttgc cttgtcgatt ctggcaacag
    16381 acactgaccc ggcgctgatc ggcggattcg ggtatcgttc cagccgggat gcagacaaat
    16441 ttgccggcgt tcccagcgca gaacatgacg gtatgccggt gccggatgcg gcaatggctt
    16501 ggatcacctg ccgggtggta aagatcatga ccgccggaac acacaccatt tttctgggcg
    16561 aggtaacaga ctgtgagctg aaccgtgccg acggcattcc catgaccgat ctgtattacc
    16621 atacggtcat caagggaaac agcccgaaaa atgcaccgac ttatgttccg ccggaggaaa
    16681 agtgagcaga tcaatgaaaa aatcaaaaat tacttgacat ttcaaaaaaa gtgtggtata
    16741 ctgtttgtcg attgatatcg acagttcgtt tgcgccatcc tgtcgttaaa taaaaccagg
    16801 gcatccgaaa cagtaaacta ccggggacgg gattgttcct gtgtgcaggc gatgcctgtt
    16861 tgaaatgaat tttggatgca gccgtatgtt gatgcggctg catttttttg ttaggaggaa
    16921 atctgatgtt tgaactcaag acatctgcca gctttgacag tgcacatttt ctgtccggct
    16981 atcagggaaa atgcgccaat ctccatggac accgctggaa gatcgaagtg accgtaggaa
    17041 aagagacctt acaggaagag ggcaattacc gggacatggt gatcgacttc ggcgatgtga
    17101 aaaaggctgt ccgtgacctg gcagacagct atgaccatgc gctgatctat gaagagggta
    17161 cgctccagcg ggcaacggta gaggcacttc tggcggaggg attccgtctg atcgccgtgc
    17221 cgtttcgtcc cactgcggaa cagttttcca aatgctttta tgaaaaactg tcctccgacg
    17281 gtctgcctgt ccgccgggtg gcagtgtatg aaacaccgga aaactgtgct gtatacgagg
    17341 tgtgagccat gaattcgtat gcagtagctg agaaatttgt cagcatcaac ggcgagggaa
    17401 agcacgccgg ggaattggca gtgttcctgc ggtttcgtgg ctgtaacctg gcgtgcggct
    17461 tttgcgatac caagtgggca aatactgccg cagctccggc agtgcggatg acgaccgatg
    17521 aactggtggc atatgtccgg gaaactggcg tgcggaatgt gacgctgacc ggcggcgagc
    17581 cgctgctcca gccgggcatt gatgagttga ttacggcgct gggtgcgctg ggctatcggg
    17641 tggagatcga gacgaacggc agcgtttcgc tccagccttt tgcgtccctg tcgttccgtc
    17701 cgtgctttac catggattat aagctgccgg ggagcggcat ggaacagacc atgctgccgg
    17761 agaatttttc cctgctgcgc agccgggatg ccgtgaaatt tgtggcaggc agccgggcag
    17821 atctggaacg ggcgctggaa atcatccgga aatacggttt gatcgaccgc tgtaccgtgt
    17881 ttttcagccc ggtattcggc tcgatcgaac cggcggagat cgtggaattc ctaaaagaac
    17941 atacgctgaa tggagtacgg ctgcagctgc agctgcataa gtttatctgg gatcccttgc
    18001 agcgtggagt atgaggagta aaaagcatgg atacaaaagc aattgaaaca catatccggg
    18061 gcattttaga agcgatcggc gaagacccgg atcgggaagg tttgcgggag acgccgcagc
    18121 gtgtggcgag aatgtacgag gagatttttg ccggtgtgca gtattccaat catgagattg
    18181 ctgagatgtt cggaaaaacc tttgatgccc cttctcccag ccagtcccaa actgcagtgg
    18241 tcatgaaaga tatcagcgtg ttcagttatt gtgagcatca catggcgttg atgtacgnnn
    18301 nnnnnnngat cggtctgagc aaaattgccc ggatctgcga catggcgtcc aagcgtctgc
    18361 aattgcagga gcgcctggga caggatatcg cagagatttt gatggaggcg gtgggaacac
    18421 cggacgttgg cgtagtcatc accggctcgc acagctgcat gacggctcgt ggcatccgca
    18481 acgtctcatc tcgaaccaca acgaaaacct tctgcggcgc attccagacg gatcacgctt
    18541 tgcaggaatt actctaggag gatagatcat atgaaagcat tggttctttt cagcggcggc
    18601 gtagacagta caacctgtct ggggcttgct gtggataagt acggcgcaga ccaggtgctg
    18661 gcgctgtcgg tatcttacgg acaaaagcat cagaaagagg tagaggctgc cggaaaaatt
    18721 gcggcgcact acggcgtaaa gtggcagcag ctggacctgt ccgtgatttt tgcggactcc
    18781 aactgctctc tgctcagcgg ttctacccag gagattccca aggagactta cgcagaacag
    18841 ctccaggaga caaacggcag cccggtttcc acctatgtgc cgttccggaa tggtctgttt
    18901 ctgtccagtg cggcaagcat tgccttgtcc aacggctgcg aagtgattta ttacggtgcg
    18961 cacagcgatg acgccgccgg aaacgcatac ccggactgca gcactgcatt caacgatgcc
    19021 atcagccagg caatctatct gggcagcggc aaccagctgc acattgaggc gccttttgtc
    19081 acctggacaa aggcagacgt tgtgaaaaag ggtctggaac tgggcgtacc gtatgaactg
    19141 acctggagct gctacgaagg cggagacaag ccctgcggcg tctgcggcac ctgccgtgac
    19201 cgtgcggcgg ctttcgcagc aaacggtatc caggatccgg cactgggagg tgaatgaaat
    19261 ggcaggaaga agtaaagaag aaaccggcga tatcacactg ctgggcaatc agaacaccaa
    19321 gtattccagc gattatgccc cggaggtgct ggagacattc ccgaataagc atccgggacg
    19381 ggattatttc gtcaagttca attgtccgga gttcaccagt ctctgcccca tcaccgggca
    19441 gccggatttt gcgacgctgt atatctccta tgttccacga gagatcatgg tggagagcaa
    19501 gtcgctgaaa ctgtatctgt tcagtttccg gaatcacggc gatttccacg aggactgtgt
    19561 caacatcatc atgaacgatc tgattcagct gatggagccg gcatatatcg aggtctgggg
    19621 caagttcctg ccccgtggcg gcatttccat tgacccgtac tgcaactacg gcagaccggg
    19681 aaccaagtgg gaacaggtgg catgggacag actgacccat catgacctgt ttccggaaaa
    19741 ggtggataac cgataattcc gctttggaaa aaataacaac cacgaacaat ggcgttcggc
    19801 gttacaggat ttttctgtcc tgtcgtaccg gacgcttttt tgtattatgc tattgcgata
    19861 aaaagttttt ttggaatttt gcacaatttt tcttgcaatc catacggaag tatggtataa
    19921 taaaaatgtt tttttctgac actgtcggaa tatgcagaaa gaaggtgcat ttcgccgcct
    19981 ttatcatccg gcgttctgca cacatatctg cggaaaaata ataaaggaag tgaacacaaa
    20041 tggaaacgtt gacaaacgtc atcaaacaaa ttgacgcatg ggtgtggggc attccgctga
    20101 tcctgctgat cctgctgtgc ggcatctggc ttaccattcg tgtccgtgga ttacaggtgc
    20161 gccgcattgg tctcgccttg aagttcatgg taaagaatga agaagagggc gaaggcgagg
    20221 tcaccagctt cggcgcactc tgtaccgcat tgtctgccac catcggtacc ggcaacattg
    20281 tcggtgttgc aactgcgatc gctgccggcg gtccgggcgc actgttctgg atggaaattg
    20341 ctgccctgtt cggtatggcg accaagtatg ccgagggttt cctcgccatc aagtatcgta
    20401 ctgttgacaa agagggacat tatctgggcg gtccgttcta ctatattgaa aatggtatgg
    20461 gttctcgctg gaagtggctg gcaaagctgt tcgccctgtt tggcgtgctt gccggactgc
    20521 tgggcatcgg tacgattacc cagatcaacg gcattacctc tgcggcaaac aacttctttg
    20581 atgccaagag cgagcatatc gcattcacac tgtttgacac agactatacc tggacgacag
    20641 tcatcatggg gctgattatc acgatctgtg ttgcgctggt tgttatcggc ggtctgaagc
    20701 gtatctctca gatctcccag gtagttgtac cgtttatggc agtggcatat gtgctctgct
    20761 gtctgattat gatgatttgc aatgtgactg ccattcccca cgctgttgtg gaaattgtaa
    20821 agagcgcatt cggtatggat gcggttgccg gaggcgcact gggtgctatg atcgtggcaa
    20881 tgcagaaggg tgttgcaaga ggtatcttct ccaatgaggc tggtctgggt tctgccccga
    20941 tcgcagctgc cgcagcccgt accaaagaac cggttcgcca gggtctggta accatgaccg
    21001 gtacttttat tgataccatt gtgatctgca ccatgaccgg tctttccatc gtcatttccg
    21061 gcgcatggca gaacccggat ctccagggcg tacagatcac agacgcagcg ttccagcacg
    21121 gtctgccgtt cccggcacag gtttcttcct gcattctgat gctctgcctg atcttctttg
    21181 catttaccac catcctgggc tggagctatt attctgagcg ctgtctgacc tacctgcgtg
    21241 gcaaggcaag ccatacgctg acactggcat tccgctggct gtacatcgtg gcagttttca
    21301 ttggaccgta tctgacggtt gaggcggtat gggatactgc cgatgtcttt aacggtctga
    21361 tggcattccc gaacgtcatt gcattgattg cattgtctgg cgttgtggca atggatacca
    21421 aacaatactt cgcagaaaag cagcatcagc tgcggtaact gcggaatcgt atacgaatct
    21481 gaaacagatc acaggacggg agcaaaaact cctgtcctgt ttttttgtga aaattcagtg
    21541 gaaatgctgg acaactgcgc cggaatatgc tataataaca aaagtatatt ttcggcagca
    21601 tccgcaggat ttgcgcagtg ctgcctttca gaaaagggca ggtgtgtttt tacaaaagtg
    21661 gtaaaacgat ttgtgaaatt atatcgctgg gtcatgctga gcatggcagt ctgcattgcg
    21721 gttctggcaa ttgcagccgg ctataccgtg ggcgttgtca gccagaataa tgtgatcgca
    21781 cagatcgatg tcaacattgg ctcgatcgag acgtatctgg agaaaaacca caacgcaacc
    21841 cagctgctga cagacgaatt caaagaggat tactccgcca aaaccagaac cattgccatg
    21901 ctgctggcgc aggacagttc gttcatcacc gatgaccgga cgctggaaga actgcgtgtc
    21961 acagtcaatg ccgatatgat aagcgttgca gatgcagacg gcaatgtggt ggcaagtacc
    22021 gacccgtccg gcgcaggcac ttccatccgt gaggagttcc ggtcacatct ggaagaagat
    22081 gtatacaccg atgcgctgtt cctgctggat tccgatacgc ctaccatcgt ggcggcatcg
    22141 tcgctggacg gcggacacgg catggtgcag atcacatttt cagcggattc cgtggtctcg
    22201 cttttgcagg atgcagacct ggcaaacatt gcgtccagta tgccgctgta ctcttccggc
    22261 actaccgcca ttctggaggc ggacaccatg aagtatatca gctgcaccga tacggatctg
    22321 atcggacaga cagccgattt tgacaagagc agcatgaaac gcaacaaggg caagtttgac
    22381 gtgaaagatg cagacggcaa gaccaatatg gtacactatc agtccagcgg cgactacatc
    22441 attatggcga cagtaccgta tgcagaggtt taccgtgtcc ggaacatcgt tgtcggatgg
    22501 gtcattggcg gcggcatcgg catcctggca gtaacctgtc tggcactccg gatggaactg
    22561 ctccggcagg atgcgaaaaa tgcgaaaaaa taaagtacag tattgcaaag gtcatctccc
    22621 cggcgaggtg gcttttgttt ttgggaaagg aatccgctat gaaaattcgt gaagtacagg
    22681 acagaaccgc accgcttatg acggcgcttt tggcagtctg ggagaagtct gtccgggcaa
    22741 cgcatacatt tctctctgag gaagagatcg aacacatcaa gacctatgtt cccacggcat
    22801 ttcacagcgt gcagcacctg ctgactgcgg aaacggcatc gggcgagccg gttgccttta
    22861 tgggcatcac gggagaccgt ctggaaatgc tgtttctttt gccggaggaa aggggaaagg
    22921 ggctgggcag acagctgctg gaatacggca tccggaatta cggcgtcggg gaagtgaccg
    22981 tcaacgaaca aaattcccag gcagtggggt tctatcagca cctgggcttt gtcacgtacc
    23041 gaagaactga ccacgacgaa gagggcaatc cctatccgct gttgtatctg aaattgcccc
    23101 aggcatgatt ggaaaagtga ttgtcgatat tgcacattat gtattgacat ttcttgaaat
    23161 ataatgtata atatgtttgt acaccagaaa tggcagtacc aatgaaactt ttatcgtgag
    23221 gtgcatgaat tatggcaaat cgtattgtat tgaacaacat ttcttatcat ggaaaaggtg
    23281 cgattcagga aatcgtcaac gaagtaaaaa ctaacggcta tcagcacttg tttgttgcaa
    23341 cagacccgga tctgctgaaa ttcggtgtga cggcaaaggt gacagatatt ctggacaacg
    23401 ccggcattgc atacacggtt tactcgaaga tcaagcccaa tcccaccatc cagaatgtgc
    23461 aggagggtgt agcggctttt caggcgtgcg gagcggatgc cattgttgcc atcggcggcg
    23521 gctcttctat ggataccgca aaggcgatcg gcattatcat taccaatccg gaatttgcgg
    23581 atgtgcgttc tctggagggc gttgcgccga cgaagaatca cgctgttccc accattgcag
    23641 ttcccacaac agccggcact gccgcagaag ttaccatcaa ctatgtcatt accgatgtgg
    23701 aaaaacagcg taagttcgtc tgcgtggata ccaacgatat cccggcagtt gccattgttg
    23761 acccggatat gatgtcctcc atgccaaagg gactgaccgc cgcaactggt atggatgcgc
    23821 tgactcacgc catcgagggc tataccacaa aggcagcctg ggaaattccg gatatgttcc
    23881 atatcgaggc gatccgtctg atctcggaga atctccggga tgcagtggaa aacaagccca
    23941 gcggcagaga gggcatggca ctggcacagt acatcgccgg catgggattc tccaacgtag
    24001 gtctgggcat cgaccactcc atggcacata cgctgagcgc acactatgat acaccgcacg
    24061 gcgtagcctg cgcaatgctg ctgcccattt ccatggagtt caaccgggat tataccggcg
    24121 aacgctatcg tgccatcgcc aaggcaatgg gcgtagaggg aaccgagacc atgtcccagg
    24181 aggaatatcg tgacgcagcc attgccgcag ttcgcaagct ctccaaggat gtgggaattc
    24241 cggagaaaaa cgaacgcatt aaggaagagg acctggaaca gctggcaaag gatgctctgg
    24301 cagatgcctg ctatccgggt aatcccagag aggcaactct ggaacaggtc attgagatgt
    24361 tccgtatgct gatggtctga tcgtttgact ggaaaggaga taccatgaag aaagtcggca
    24421 tattgctttt gacaggaacg ctgctgctgt gcagctgcgg acaagcgaaa gaaactgcgg
    24481 aaaacagtgc agatgtcccg gaaaccattc agacgaatct gacggcaaat ccttattttg
    24541 atatttctga tctgacttat gacggcacag aaacgcagaa tatcgccatt ggatattcct
    24601 ttgaagatcc ggcagacaat gaccctgttc tgacctatga ccgctatctt tcggcggacg
    24661 gcgagacgtt ctgttttgat gctgacggac ggctttgcaa gtatgagaaa ggcgatgctg
    24721 atacagctga aaaagcagaa agcggttcga tgacggcagc atacacgatg gcgcagcgcc
    24781 aacatcgcag tctcgatgtg gcagagagcc tgctggcgga agatacccaa ctggaagtct
    24841 cctctttcgg cagcgaatat ctggtgacca acgatgctgt tacaccggaa aacgaggcgc
    24901 cgttctctgc cattgtggat gtggacagca aaggagaagt gcgtcagctc agtgtcagct
    24961 ataacacgct gtctgcgcct atcgactatg actatttcca gcaaaagctg gatgcgtata
    25021 tcgaagaggc aaagcagacc tgggatattg tggactatac agtagaacca cggtatcagc
    25081 agatcggcaa taagatctac gccatgtata cggtgacgtt tacagaggca acagatgagc
    25141 cggaaggtgc aaatttctgt gaatgcgtcg gctttaccca gaaaattgag agttagttcc
    25201 ggtttttaca attttttctt gccctgttgt ggtaagataa tactcgaaca aaggagttca
    25261 gatgaatcag acatctgaac tcctttttta ttttgtttgc atggaatcgt tccggtacac
    25321 acaggcgtgc gccggcgttc cggaggaggt atttcagatg agctatccag aagttgattt
    25381 tttgtatctg aacgaagagg atatggttgc agccggtgtc aaggatatgg caggctgtat
    25441 tgatgcgatg gaagaaatgt tcaagctgat gaaggtgggc gactatcgca tgggcggcgc
    25501 aaacggcaat tcccacggat gtatggtcat gtttccgccg tcctctcctt tcccggaaat
    25561 gccggtagac ggaccggaca gacgctttat ggcgatgccg gcgtatctgg gcggtgtgtt
    25621 cgatatggca ggcatgaagt ggtacggttc caattgtgcc aacaaggaaa agggactgcc
    25681 ccgttccatt ctgatgctga cgctgaatga caaagacacc ggcgcaccgg tggcatatat
    25741 gtccgccaat atcctttccg cataccgcac cggcgcagtt ccgggcgtgg gatacaagca
    25801 cctgtcgaaa ccggattcca aggtagctgc cattatcgga ccgggtgtta tgagcaagac
    25861 ggcgcttgca gcgatcatgg cagtccgtcc cggcatcgag accattaagg tcaagggcag
    25921 aggaaaggca tccctggaaa agttcattgc ccatgtgaag aaagaatatc cccaggtgaa
    25981 tgtgtatgct gtggatacca tggaagaggc agtccgggat gcggatattg tttcggcaag
    26041 cacctcaacg cctaccggag accccagcca gtatccgtac atcaaggagg agtggatcaa
    26101 gcccggcgca ctcatcagtt ctaccgcagc gctccggttt gatgatgact ttatcatcca
    26161 tcgtgcaaga accgtgacag acaacattaa gctgtatgag gcgtgggaag aggaaatgaa
    26221 gcccaacgcc tacaatacag ttccgattcc ggcagttcac gtgatggatc tgattgcaga
    26281 gggcaggatg aaaccggagc aggtggatga cctgggcgat atcctgatgg gcaacattcc
    26341 gctgcaccgg aaggatgacg agatcatcgt gtactccatc ggcggtatgc cggtagaaga
    26401 tgtggcttgg ggtacgatgg tttaccgcaa tgcactgaaa aagggcatcg gcacaaagct
    26461 gaagctttgg gatgtcccgc agatggtata aataagacag gaggagcaga catgagaata
    26521 caggaacatc cgattctggg aacatatgaa aagggacagc tggtttcctt tacttttgat
    26581 ggaaaaacac tggaaggcta tgccggagaa cccattgcca tggcactgaa aaatgccggc
    26641 gtaatggttc accgctatac cagaaaagaa cataagccga gaggaatctt ctgcgccatc
    26701 gggcgctgta ccgactgtgt tatggtcgtc aacggacagc cgaatgtgcg gacgtgcatc
    26761 acgccgctgg aagagggcat ggagatccgg acacagtacg gtgtgacagc agaacagccg
    26821 ggaggtgcag caaaatgatg aaacgttatg atatgatcgt ggtcggcgca ggaccggcag
    26881 gtttgtccgc agccattgag gcggcaaaaa aaggtatgca cgttgtagta tacgatgaaa
    26941 acgcaaagcc cggcggacaa ctgttcaaac agatccacaa gttctttggc tcaaaggaac
    27001 acaaggcgaa gatccgtgga ttccatatcg gcgaagaact tctggcagag gcagcgtctc
    27061 ttggtgttga agttgttctg aatgctatcg tgatcggcat tttcccggaa aaggaagtta
    27121 ctgtaaaggt ggaggatcat gtgatccacg tcaagggtga taccattctg atcgccaccg
    27181 gcgcaagcga aaacatggtc acattcccgg gctggacact gccgggcgtc atcggcgcag
    27241 gcgcagcgca gaccatgatg aacctccacg gcacactgcc gggcaagcgt gtgctgatgc
    27301 tggggtccgg caacgtgggt ctggtggtca gctttcagtt gatgcaggca ggctgcgaag
    27361 tggttgccgt ggcagatgca gcgccccgtg tcggcggcta cggcgtccat gccgcaaagg
    27421 tcgcaagatg cggcgtgccg ttctatctgt cgcacaccat catttccgct gacggcgagg
    27481 attgcgtaac aggcgtgacc atcggagaag tggacaagaa ctggcagatc attccgggga
    27541 cggaaaagca ctttgatgtg gataccatct gcctggcggt aggactgtct cccatgtcca
    27601 agctttgcga tatggcagga tgtgccatgg aggacaatcc ggcaaaggca ggatttgttc
    27661 ccacagtgga tgaatacggt gagacctctg ttcccggcat ctatgccgcc ggcgatgttt
    27721 ccggcattga ggaggcatcc agcgccatga tcgagggaag gatcgccggc attgccgcag
    27781 cggcaagact gggattcatc tcagaggaga cctgcaaaga agagactgcc aagcaggagg
    27841 ctgcgctggc tggtctgcgg cagggaatgt ttgcaccggg aatgcgtggc aagcagatca
    27901 ccgagaccga agagggtgtg gaaatctcgc aaaacctgtt gaaacatggc tttgtggcag
    27961 aggaagagat cgagcgtttc cctggtgtga cccacagcag cggcgtgcat ccggtcattg
    28021 aatgcaccca gaacattccg tgcaatccat gtcaggatgc ctgccccaag cactgtatcc
    28081 gcattggcag caacatcaca gcgctgccgg caatcgaccc ggagaaaaaa tgcactggct
    28141 gcggaatgtg cgtggcatcc tgttccggtc aggcaatttt cctggtcaac gaggactgcg
    28201 gcgacggcac tgcagaagtg acactgccgt atgagttcct gcccctgccg aaaaaaggtg
    28261 acaagggcgt ggcattgaac cgaaagggcg ataaggtctg tgacgcagag gtagtcaatg
    28321 tgcggagcgc aaaggcgttc gaccatacca atctgcttac catgaaagtg ccgaaagaat
    28381 atgccatgaa agcaagattt tttcgggagg aggagacagc atgaacgaca gaaaccgtga
    28441 actgaaaccg tttgttcctc agccggatga cgatatgatc gtctgccgct gtgaggagat
    28501 cacgaaaggg gagatccggc gtgctgtgca tgacggtatg tttaccctca ccgagatacg
    28561 ccgctatctg cggacgggta tgggactctg ccaggggcag acctgtacaa aactggtaaa
    28621 aggcatcgtc gcacgggaac tgggtgtgcg tccaaacgaa ctcggacctg ctaccggcag
    28681 agcgcccatg cgtcccatcg aaatgaaagt gctggcaaga gataaggagg agacaaaatg
    28741 aatgcagaag tcattatcgt tggcggcggc atcatcggat gcgctgcggc atataacctt
    28801 gcaaaaaatg gtgtctccgt cctggtgctg gagggcagcg aaaatatcgg aaacggcggc
    28861 tctaccagaa acggcggcgg cgttcgccag tccggccgtg accccagaga actgccgctg
    28921 gtgatgtact ccatccagca catctggccg cagctgtccg atatgctgga caccaacgtg
    28981 gagtactacc aggaaggcaa ccttcgtctg ggcagtacgc cgcagcatca gaagatcctg
    29041 gaaggtctgg cagaccgtgc cgcagcctgc ggcgtggatg tgcgcatgat ctccggcgat
    29101 gaggcgagac gcatcaaccc gtatctgtcc gacgctgtga cctgtgcaag ctggtgtccc
    29161 acggacggtc atgccaatcc gctgatggca accatgggct actataagat ggcacgccgt
    29221 ctgggtgcgc acttcatctc cggtgagaaa gttgtggagc tgcggaagat caagggcgct
    29281 gcacgacagg tggtgaccga atccggcaat gtgtatgagg gtgacaagat catcctggcg
    29341 gcaggctatg agagccgttt cattgcatcg actgtgggca ttgatgttcc catgcagccg
    29401 gtgctgctgg aaacgctggt caccgaggca gtcgctccga tgttctggca gatgctgggt
    29461 actgctgcag ccgatttcta cggtcaccag acagagcatg gctcgttcgt ctttggcctg
    29521 aactccggtc tggaaccgta tgcaaaaccg ggcaagcccc tgtcctccag catcgcagca
    29581 tcggctgcct gccgtggcat catgcactat ttccctgatc tggcaaacat caaagtcgtc
    29641 cgcagctggg caggctggat ggatgcctgt gcagatcatg tgccggttat cagcaccgtg
    29701 gacgaagtgc cgggactgat tctggcgtgc ggctttacgg gacacggttt cggcatctcg
    29761 ccggctgtcg gtctggttct ggcgcagctg gcaatggagc aggaaacggc agtggatctg
    29821 tccgcactgc ggtatgaccg ttttcagtaa gaggttctgt tttttacaat tttttagaga
    29881 gcgcatctgt tataataaga gtgcagaaag acaaagatgt cgaatttcct ggaaattcgg
    29941 catcttttct tttttgggaa aattttttga agttgttgga ggaattcgtt atgaaattat
    30001 ccagagttga gatcatcacc ggcatttcca aaatcgaacc gctgaaaaag gcgctgggaa
    30061 agttccatat caccggtatg acggtatacc aggtgctggg atgcggcgta cagcacggta
    30121 cccaggaatt tgaggtggag gagaagaaca aggagatcag tctgctgccc aaagaggtcg
    30181 tcatgatcgt tctgcccaca gaagaactgg acgggctgct ggaattcgtc aagaaagaac
    30241 tgtacaccgg gcatatcggt gacggcaaga tcttcgtctc ggatgtgacc aatgtcatcc
    30301 gtgtccgcac cggcgaagag ggcattgacg cactgctgta cggtgaaaaa taaggatttg
    30361 tcccagaaaa gagttgagag attatgcagg agaaaaaact aggcttaccc agtattgtgg
    30421 caactggcgt agggttgatt attgccacca gctgtctgct ctccatcggg cagggcgcat
    30481 ctgccatcgg actgccgttt atcatcacca tggcgattgc ctgtgcattc aacattctga
    30541 cggcgttttc catctgtgag ctgaacgcac tgatgccgaa tttgaccggc ggtatggcgc
    30601 agtatacgct ggcgtgcttt ggaccgtttg tttccattgt cattaccgtc ggcggctatc
    30661 tgacctgtca gaccatcatg ggaagttctg aggcggcaat gttcggcaac acactgaaca
    30721 gcgtctttac cggcgttccc attccgggag cggtgtattc cattgtgctg attctgattc
    30781 tgctggggct gaacctgttc ggcgtggata tgttcgcaaa ggtgcagaat attgtggcat
    30841 acggactgat cggttcgctg ctgttcctgg gcatcatggg tgcgctcaag ttcggcacag
    30901 gacaagtggt ggaacagccg gaggtgcttt cctcaaaacc cggcgatgtg ttctctctgc
    30961 tgggactggc gttcttcctg ttcatcggcg tagagttcat tgttcccatt tccacccagg
    31021 tcaagaatcc ccggaaaaaa gtgccgctgg gcatggtcat cagtctgctt gcaattctgg
    31081 tcatgcaggt ctttgtggta attggtttca gccactatac accgtgggag gagctgagcg
    31141 tcagcaccgt acctcacatc ctgtacggca ctctgctgta cggcaaggtc ggcaccatct
    31201 ggatgacagt catttctctg ctggcagttg tcagcaccct gaacactgct atgttctcca
    31261 ttgcgcagat ctgttccggt atggcaaagc tgaaactgct gccctctgtg ttcatgcgga
    31321 agaatcagcg tggcacaccc tatgtgggac tgctgttggt agcggcagct atgattctga
    31381 ttaacgttac cggactttcc accagcgacc agctgacgtt cctgattctg accggatgta
    31441 ccttctggat cttctcgtat atcatcctgc actgcgatgt gctgatcctg cggaaacgtc
    31501 tgccgaaggc accccgtacc tttaagctgc cctttggtcc ggtgatcccc atgatcggca
    31561 ttctgggcaa cgcctttatg atctacaata ttgactccag ctgggaggtc aagaaacgga
    31621 tctatctgat ctttgtgata acggtagcga tcctgtcggt ctatgctgtg atttggatca
    31681 agtgtgtcat gaaacgcctc atgttcaagg cgtatcagat caaggaagtc atggcgatgg
    31741 aatccgacct gtaccagatc aatcataatc cggaggtggc gaagaaactg cacctggatg
    31801 cagttccgga gatcgagagt atgccggcgg atgtcaacac cggtactcgt ccggaataag
    31861 cgcctgttgc cttaggacaa ctttccataa aaaagcacac actccctgtg aatattctgt
    31921 cgaaaaggca gtcttacgga aaaaaggttt ttctgtaaga ctgccttttt ttcatcagct
    31981 gcacttgaaa aatgccggca tctgtactat aatatgagag aaatcaccgg aaggagggaa
    32041 gtactgcata gaaaaacaga tctacatgac accgaaacag ccgtttttcg tgacctcttc
    32101 ccgttcttat gaaaagctta ttttgaaccg gtttggcatt gttcactttt accactgctt
    32161 taccggtaca gaaccccgat atgcgattcc ggatggatgc gtggacatgg tgttctgctg
    32221 tgacccgaac catccttatg cggaaatctg cggcacagtt ctttcgcccc aggtggtgct
    32281 ttccgagtgc aacaccacct atttcggcgt gcgtttcctg ccggggtata atccggttct
    32341 gggcagtgcg gatattatgg cgcatctggt caaccaccgc attccgctgg atgatctgat
    32401 ccaggacagg cggatgctgg agggtatttg gggcgcatca gatttccggc aacagatccg
    32461 tgtgtttatg cgttcctaca tgaacatcta tcgccgagtc tgtccccagg aacatcagaa
    32521 tctgctggtg ctccactctt ccaatttgat gatccgctcc ggcggcggga tttccgtgga
    32581 acagctggcg gaggataccg gttatacgcc cagatacatt gacaagtgtt ttcggaatga
    32641 gaccggactc agccccaaac agctggcgaa gatcgtccgg tttcaaacgg cagtgtctgc
    32701 gctgaaccag aaccagcaca cggggcgctc tctgaccgag atcgccgccg acctggggta
    32761 ttttgaccag tcccattttg tacacgattt caagacatat accgggctga caccgaaaaa
    32821 ataccagatg ctgctgcgga acgatgcctt tgacgaaaag ctgcgcattg tgcagtaaaa
    32881 aaacggtttc tctttggaaa agggaaaccg tttttgtcgt ttttatgatt ctgaggaaat
    32941 gctgtgttcc ggctggtaca ggatcacgtt gacctcgtac cagtcctttt ccggcacagt
    33001 ccagattttt ccatgatagc ggtctacaat gtcctggaca cttttcatgc ccagaccgtg
    33061 ccgctttttg tcgggctttc tggtaccgct ttcatccggc aggatgttgt catagcggtt
    33121 gcgtatgttc agctgcaatg cgccgaactt tacccgcatt ttcagaatga tatgccgctg
    33181 ttccttcgga aagacctttt cagtcgcctc tgcggcgttg tccagcagat ttcccagcag
    33241 cacagtcaaa tcaaacggcg catagggcag ttcttccggg agacgaatgt caatgtccag
    33301 gttggtatca tccagctgca gcggcatgag tttgtagttc agaatgctgt tgatctgctc
    33361 gtgctgtgtg tacgtggtac tgcgtgccag gtctaagtga tattccgtgt cgctgatgta
    33421 gctgcgcagt ccgtcgatgt cgttggtgtc cagatagtgc tgcatctgaa acaggtggtt
    33481 cttgatgtcg tgccgcagaa accgcagccg catctgagac tgctgtaccg ctttgatctg
    33541 ttcctggtat gttttgttct gctgttcgat cagatgaacg gtgtgctgtt ttttcatcag
    33601 accgatcaga tggtcgtaca gagagaaaac gctgatattc aaaagcagca ggatgatggc
    33661 tgcgatgagc gagcgccagg agagcgtccg ggcaatgtag tgcgccagaa agatactgcc
    33721 caggggaata aagagaatgt tgataacgta aaatataccc agcctgggga caacttcttt
    33781 ccggtacagc ttgaaatgtg cggcaatgcg tgccacgatc agcagcagaa gcgctgccgc
    33841 aaatccgctg gtaaagaaca gggtatagtc aaaccagatg gagactgtgg cgcagagaat
    33901 gtcgatcgcc atgctgacga tatagatgga aaaaacgctg aggatgcggt gtgaccaggt
    33961 agtgggataa aacaaggtga tgcagaaaaa cggaatcaga ttgctcagaa cattccatat
    34021 gatgctgtgg aaatagacaa agcaaaagct gttgatctgg aaatagagaa aatacaggat
    34081 aaactccagc agctttaggt tgggcttttt ctgaaagagc agctgtgaaa actgatagac
    34141 agcatagata tgaaatatgt tcgcaataat ggtgatgtaa tgtgccatag gcttagtgct
    34201 gcatcagcca cttttcaacc agcggctgat aagtccggct gatggggagc tgttcgcctg
    34261 cctttccctg caagtatacc gtgtttgcgg aaatgtggtc gatatagtcc gggttgacga
    34321 cgtaggattt gtgaataggg acaaagtgcg caaaacatgg ctttttttgt acctcgctga
    34381 gctttccgta aaagctgatg gtggtctcat tggtttgcag atagatcttt ctgccgcagc
    34441 tgttcagata ccggatcttt gagacctgga cgtgtaccgg cgagttgttg caggtaaatt
    34501 cgaatacacg ccatgagatg tcccgataat atagtgcatc gtccagtgcg tttgagatct
    34561 tctcataagt caccggcttt tgcaccaatg ctgccggtcg gttcggaatg gtgtggatgg
    34621 cataattttc aaaagaggtc acgtagataa tgtcaaaata attatgtccc agccgattgc
    34681 gcagatgtgc gcccagtgcg atgccgttat cgcatcgctc ccgtgttgtg tcaaataagc
    34741 tgaaatgctt taccggttct gttccgccgg aaaagtcgat atccatagaa acaaggtcat
    34801 agcgtttttg ggtgaagtcg gtattgtaaa gcgtggcgca gtccgtataa atgtccgggt
    34861 catctatgtc gatgtccttt tgggtgctgt actgcatcag gcattcctgg atctgctggc
    34921 aaatataccg gtcatcatct gcaatcgccg tctttagggg aatcatgtga cgttgaacct
    34981 ccgtttcttt tttggcgtct gctctctgcc ggaaggacat ccggctctga aacaaacccg
    35041 tttcggcaaa gctgggttgc cgctggaact tcatattagt tggaaaatat tcctactaaa
    35101 gttatagata tttcataaaa ttattttcta caagaaatgt ttgaactaca tgaagtttac
    35161 tattattata ccatgacgta ttgaaaattg caatagaaat tcatgttttt tccattgcag
    35221 aaggctcgat tcgacaggat tcgacaaaat tcgcaaaata ttccagcgca aaaacgatgt
    35281 gtcaacgcag aaaaatcgaa aaaagatgac agttatgcag ggcggttagc cgatttgaca
    35341 tttgcattcg aatgtgctat aataggcaat gtttgcagaa agaaatctga ttccgtggaa
    35401 atgggggagc ggtcgtgttg gaacgttttt tgcggcggtt tgtgccgaat tatgaaagtg
    35461 tcgaggaccc ggcggtgcgg aaagcttatg ggacattcag cagcgtggtg gggatctgct
    35521 gcaatgtggt gttgttcctg ctgaaatatg tgatgggttc gctttcccac tccatcgcca
    35581 ttgtatcgga tgcgttcaac aacctgtcgg actgtgccag ttgtctgatt accatgttcg
    35641 ggtataagct ggcggcaaag ccggcggaca agggacatcc gttcggacat gggcgcatgg
    35701 aatatctgac ctcgctggtg atcgccgtca tgatcgccct gatgggcttt gaactgctgc
    35761 ggaattccgt ggaaaagatc atccatccgg atgcggtgca gtttcgctgg ctgaccctgg
    35821 gcgcattggt gctttccatt ggggtgaagt tgtggatggc atggttcaac acccggctgg
    35881 ggaaaaagat acgctcttcg gtgatgatcg ccacggcgaa ggacagccgc agcgacgtgg
    35941 cagccacggc ggcaaccatt ctggcactgc tgctctctct ggtgacaacg ctcccggtgg
    36001 acggcatcat gggtacggcg gtctcgctgt ttattttgaa aacggcattc gatatcatac
    36061 gtgatacggt gggagatctg ctggggcgac cggcagatgc cgagacggtg cagcagctga
    36121 aaacgctggt gctgtcacat gataagatcg agggcattca tgatctgatc ctgcacaatt
    36181 acggtcctgg acgcaccatc ggcagctgcc atgtagaggt gcgcagcgac tccaattttg
    36241 tggaggtaca cgaggtggtg gataccattg agcgtgagat cgaagagaaa ctgaaaatca
    36301 tgatgaccat acatatggat cccattgaac tggataacga aacggtcaac cattaccggg
    36361 agatgacaca gcaggttgtg gaaggactgc atcagaaact gagtctgcac gatttccggc
    36421 tggtgtcggg ggagaactcc aaccggctga tttttgatct gctggtgccg ttcgagtgtg
    36481 attatacaga tgaggaactg ttggagatga tttccggaga gctgcaccgg atggatcaga
    36541 cctgttctgt cagcattaca tttgaccggg actttacaga ggaataaagg cagcactgca
    36601 aaacaaaaaa cgtctccgta tctgttttgg aacgatacgg agacgttttg tcttttattt
    36661 tacactttaa gattgatgaa ccagatcgac aaagcgcata aactgtgcct ttccggtttc
    36721 tgttcgcttg aatacgccgg catccagcag aacctgttcg aatactgcgc cgatctctcg
    36781 gcggatgatg tccggtgcag tttcagcgtc tgcctccgga tggcgagact tgacctcttc
    36841 cagccagtct gcgtggctgg cgagagacgg cgtctgagta aggtcggtgt cgctgtacaa
    36901 cgccttttcc aacagcggca gttcgttcgc aaggcgagcc ggcagaactg ccagacccat
    36961 aacttcgatc agaccgatgt tttccttttt gatgtggtgc agggatgcgt tggggtggaa
    37021 cagtcccagg ggacgttccg gtgtggtacg gttattgcgc agtaccaggt cgcattcata
    37081 cagcctgcca cgcatccggg cgatgggggt aatggtgttg tgcggtgtgc cgtcagtctc
    37141 tgcaaagata tccgcctcag catcggtgta atttcgccac agtgtcagca catggtcgca
    37201 ggcggcagca agtgcagagc gattttcact ttgcaggcga atcacagaca tgggccactt
    37261 tacaatgcct gcctggactt ccggctggtt cggcagcagg aacggtgtct caatgggcgc
    37321 ttttgccatg gcgaaggtgt aatgaccgcc ctggaaatgt tcgtggctca gaatcgaacc
    37381 gcctacaatg ggcaggtcag cgttggagcc aatgaaataa tgggggaaca tatccaggat
    37441 atcgaacagc ttttcaaaaa cggcggcgtc gatcaccatg gggatgtgct tttggttgaa
    37501 cacgatgcag tgttcgttgt agtagccgta aggggaatac tgaaatgccc agggttcgcc
    37561 gtgtacctga atcggaatgg gacggagatt ctgccgtgcc gggtgcgtca gcgttccggc
    37621 aaagccggtg ttttcgatgc agagctgaca cgccggatac ttggtctggg gctggttctt
    37681 ggcggcggca atgtctctgg gatctttttc cggcttggaa cggttgatgg tgatatccag
    37741 cgtgccgtaa tcgcagtcat acgtccagcg catatccttg gcaatccgtc cggcacggac
    37801 atagttggtg tccttgctgt actgatagta ccagtcggtc gcctcctccg gactcttgtg
    37861 gtatgcggtg aaaaatgcac ggtttacggt gtgcggcata tcggtcagca gtcccatgat
    37921 gcgggtgtcg aacagatccc gtgctgcgtt ggaatccgca atgatgccgt tttctgtggc
    37981 ataggagacc agtttgtcca gaatctcgtc gatcgagggc aggacctccg gcagcgccgg
    38041 tgtttcccag gaagagagcc ggagcagatc catcagctga ttgcgcagga aaatgctgtc
    38101 ctcttccgtc accagctctt ttgcaatgcc gtactggatc agttcctgaa tgctttgttc
    38161 gatcatcagt gttccctcct gttatgcaag atacagttcc ttgacagccg cttcgccgct
    38221 gccctggaag aacagataca gcggatgctt gccgtcagcg atttgcaggg cggttctgac
    38281 ctcgctccat gtctccgccg gcgttaccgg aatttcagct acgatatttt tgcagccgat
    38341 gtcatccgat accacgaact taccgctgca cgtgccacgg tactgaatgc cgatctcctt
    38401 tgcgccggaa aactggaaat acttgtagcc cagcatggtg ccgtcttgga tctcgcccac
    38461 aaagcgatcc tcgccgcagt ttgtcacgtt tgggaaacta tcggtgtaga tcttgttgga
    38521 accgtggggc atatgaccgt tggtgatgtt gcaggcgatc actgccggat aagttccctt
    38581 tccctccagc ggcttgccgt tgagaccgca ggaggttatc tctacctggg ggatgctgcc
    38641 gtccggcaga atggtgatgg gttctgcaca cgcctggcgg ctgtagtcgg atttgtgagt
    38701 cagacggtga tagaaaacgt accactgacc attgatgttt tcgatgctgc cgtgggtcgt
    38761 tccggtcatg ttcaggcggt ctttttccgc tctgccctgg tatcccacat cgccgttgga
    38821 aacgatcgtg ccgccaaaga caaagtcacg gtcgggatag tcgctggtgg catagcacag
    38881 ctcgtggttc tgccaggagg aatagatgaa gtaatacttt tcgcctatct tgcggataga
    38941 gcttgcctca aagaacggat gcgccgcaaa gctggtgccg gtgacatctg ccgggataat
    39001 gtgtctcggc tcgctggtga cagtcatcat gtcgtcgcct acggtaactg tgcagggtcc
    39061 catcacactg ccctcggtgt ccagaacttc gttgcggctc ttgtgatagc gctgcataac
    39121 ggtctggatc agatcttcct tgttccggaa atccttgtcc agctgttcgt ccgaccatgt
    39181 gccgtagtag atgcggatgg tgccgttgtc gttgataacg cccgggtcaa agcagacata
    39241 cttcagcata gggctgccgt ccggatagcg gacgaaaccc agatagcgga acggaccagc
    39301 cggagagtca ctgacagcca cgctgatcgg accgttgtag ccgccgaagc cggctctgcc
    39361 ggacatacaa taataaaggt agaatctgcc gtcgttgccc tgtaccacat ccggggcata
    39421 catgaatttg cggtctgtgg ggtatgccgg atcctggtcg gcacgataga tcgtaccctc
    39481 acagcgccag tcggaaaggt cggtcaccgg tgcagaatat gtggtgtagt ccagcataca
    39541 gaaggtctcg ccgccttctt tgtcgtgtga gccatagatg tatacacggt cgccgaatac
    39601 gtgcggctca ccgtccggaa tacattcata caggggaaga tacgggttgt aaacttgttg
    39661 tttcataagc gttcctttct atcgccgacc tgcatcagaa cctgtgcaga atcatatttg
    39721 tttgcttaag agtctgtttc gtatcttttt tggaacgtct tt
//
LOCUS       DAWENO010000003        35790 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_481_length_35790_cov_9.955394, whole genome shotgun sequence.
ACCESSION   DAWENO010000003 DAWENO010000000
VERSION     DAWENO010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 35790)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 35790)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35790
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_481_length_35790_cov_9.955394"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8445..8454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8787..8796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11534..11543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17951..17960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18477..18486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20738..20747
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23104..23113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8767 a         8577 c        10226 g         8150 t
ORIGIN      
        1 ccggatgtca aggtcaatac ctaccggact ttcgtcacca gcgaaaacat catggatctt
       61 atcaaggact atgatttcgt aattgacggc acagataatt tcccggcaaa gttcctcatc
      121 aacgatgcct gcgttatggc aaagaaaccg ttctcccatg ccggaatcat ccggttccag
      181 ggtcagctca tgacctatgt gccgggagaa ggtccctgct atcgctgcgt attcaagaat
      241 ccgcctccga aggatgccgt accgacctgc aaacaggctg gtgttatcgg cgcaatgggc
      301 ggcgtgatcg gcagtctcca ggcaatggag gcaatcaagt acatcctggg catcggcaac
      361 ctgctgaccg gctatctgct gacctatgat gcactgaaaa tggaattccg caaggtcaag
      421 ctgccgtctg aaacatccgg ctgcgcaata tgcggtacac atccgtcaat cacagaactg
      481 atcgactatg aacaggcaga atgcgatggt gttggattat gattcagttg aacaaatcag
      541 attttgaaaa aatgctggca cacgccagaa aagaactgcc ggacgaggcc tgcggtctga
      601 tcgccggcga gatcaacgga acggataaga tcgtcaagaa ggtctacctg ctgacgaaca
      661 tcgaccattc caatgaacat ttcagcctgg acccgaagga acagctggca gcagtcaagg
      721 atatgcgtgc caacgggctg gttccgctgg gcaactggca ctctcatccg gagtcgccgt
      781 ccagaccttc tgaggaagat aagcggctgg cgtatgacag caaggcaagc tatatgatcc
      841 tctctctgat ggatcgggaa aatccggtgc tgaactcctt ccatatcgaa ggaactgacg
      901 cagaaaaaga gaacttgcag atcaaggagt aagctatgta cgacatcatc atcatcggca
      961 gcggtcctgc cggactgacg gcggcaattt acgccagccg tgccagactc cggacgctgg
     1021 ttctggagcg tgacaccatg ggtgccgggc agatcgctgt gacagagcag gtggacaatt
     1081 atcccggact gctgcacatc ggcggctatg aactgggaga aaagttccgg gaacacgcag
     1141 agtcgctggg cgcatcaatt tgcgaaaatg atgtagagcg gctgaccaag caggacggcg
     1201 tgtttcagat cgcactggca gatggtacga ctgtttctgc aaagagcgtg atctatgctg
     1261 ccggcacatc ttaccgaaag ctggatgtgc cgggtgcgga actgccgggc gtgtcgtatt
     1321 gtgccacctg cgatggtgca ttctaccagg gaaagacagt tgcagtcatc ggcggcggcg
     1381 atacggcgct gggagatgcc ctgtatctgg cacgcatggc aaagactgtc tatctggtac
     1441 accgccggga tgctttccgt gccaatgcag cattgcagga atcggtaaag cagacggaaa
     1501 atattgttct ggtgacaaat gcagttccga ctgcggttgt gggcgaaaag cgtgtttcgg
     1561 cactgcggat cgaccataac ggtcagccgg aggagcttat tctggacggc gtttttgtgg
     1621 cgatcggcag tgtgccgaac acagagccgc tgcgtggtct ggctgacctg gatgcacacg
     1681 gttacgtcaa ggctggcgag gacggcatca cttctctgga tggactcttt gccgccggag
     1741 atgtccgcac aaagctcctg cggcaggtgg ttaccgctgc ggcagacggt gcaaactgcg
     1801 tggcatcggc agaacagtat ctgctgcaga atgaaaaaag atggtgatga gatgaaaata
     1861 gaacgctgca aaacagacgt attgattatc ggcggcggca cggcaggctg ctatgcggcg
     1921 ctgaccattg cagagcagtc ggatgcaaaa gtgctgatct gtgaaaaggc gcacattaag
     1981 cggagcggct gtctggcggc tggcgtcaat gcgctgaatg cttatattac cgagggcaga
     2041 aagccccagg attatgtgga ctatgcccgg aaggatgcag acggcatcgt ccgtggagat
     2101 ctgctgctca ccatgtcgga acggctcaac aaggtgacgg aacacatgga acgtctgggg
     2161 ctggtcatcc tcaaggatga aaacggaaag tatgtggcac gtggcaaccg gaatctgaag
     2221 atcaacggtg aaaacatgaa accgatcctg gcaaaggcag tggaatccct gccccaggtg
     2281 acgatcaaga atcacgtgaa catcacagac tttttcgtca aggacaaccg cattcaaggt
     2341 gcgttcggct ttggcatcca ggatgacaac gtgtatgtga tcgaggcgaa tgcggtgatt
     2401 atcgcaaccg gcggcgctgc cggactgtac aagcccaatc atcccggctt ttcccggcac
     2461 aagatgtggt atccgccgtt taacacggga gcaggctacg ccatgggtat ccgtgccggc
     2521 gcagagatga cgacctttga aatgcgtttc attgcactgc gctgcaagga caccatcgct
     2581 cccacgggaa cgctggcaca gggtgtcggc gccaagcagg tgaattcgct gggcgaagtg
     2641 tatgagacca aatacggttt gactacttcg gagcgtgtct acggcacggt cagcgagaac
     2701 cttgccggaa gaggtccctg ctatctgcgg acagagggca ttacaccgga acaggacgat
     2761 tcgctgatga aagcgtatct gaacatggca ccgtcccaga ccttgaaatg gatcgagaac
     2821 ggaaagaatc cctcgcagca gaatgtggag atcgagggaa cagagcctta cattgtgggc
     2881 ggtcataccg ccagcggcta ctgggtcgat accaagcgtg ccacgacagt gcatggcttg
     2941 tatgccgccg gagacgttgc cggcggatgt ccccagaagt acgtgaccgg cgctctggca
     3001 gagggcgaga tcgctgcgga gtctgttctg gaatttctga aagaaaacca gccgcagtcg
     3061 ccggaggaac aggatgttca ggcgcatctg gcagaactgg aacattttct gaatgatgcc
     3121 ggcggtctgt tcgataccga gcagctggaa gatgccatgc agacggtgat ggatacctat
     3181 gccggcggca ttggcacaaa ttatcggttc cacgaaaagg aactggatat tgctgatgat
     3241 aagattgcac ggctgacgga actgtccgat cagctgcacg cagacgatta tcaggaactg
     3301 atgtatatat atgagctgcg ggagcggctt ttggtgtgcc ggagcgttat cgcacatttg
     3361 aaggcgagaa aagaaacacg ctggcacagt ttcgccgaga atctggatca tccggagaag
     3421 gacgatcaga actggcgcaa atatgtgaat tcccggatgc gaaatggcaa gctggaagtg
     3481 gtcttccggg aactggtcga ggagggacag aactatgagc attgcaattg atgcaaagca
     3541 gtgtgtcggc tgcggaaagt gccgcatggt ctgccccggc agcttgattc acatgaacca
     3601 ggacggaaag gcattcatca agtatcccaa ggattgctgg ggatgtactt cctgtctgaa
     3661 agagtgtgcc gttcacgcca tccgctttta tctgggtgca gatatgggcg gcatgggcag
     3721 cctggtacac acggaaaaag agggcgatat tttacgctgg atcgtcgatc accccaacgg
     3781 gaaaacttcg gagatccgga tcaataccaa acagtcaaac caatactaag gaggaaaacc
     3841 ttatgcagga attgtcacat cttgacgaac tggaagctga ggcgatctat atcatccgtg
     3901 aggtcgcagc agaatgcgaa aagccggtta tgctgtattc gatcggcaag gacagctccg
     3961 taatgctgca tctggcgatg aaagcattct atccggaaaa gccgccgttc ccgttcttac
     4021 atattgatac cacctggaag ttcaaggaca tgatccgttt ccgggatgag accgcaaaga
     4081 aactgggcat tgagatgctg gtttacacca atgaagaggg cgtcaagcag ggcatcaatc
     4141 cctttgacca cggcgcagcc tataccgata tcatgaagac ccaggcgctg aaacaggcgc
     4201 tgaccaagta cggctttacc gcagcattcg gcggcggcag acgtgacgag gaaaagtccc
     4261 gtgcaaagga acgtatcttc tcgttccgca atgaggcaca ggcgtgggat ccgaagaacc
     4321 agcgcccgga aatgtggaag ctctacaaca ccaagatcaa aaagggtgag agcatccggg
     4381 tattcccgat ctccaactgg acagaaaagg acatctggca gtatatcaaa cgggaaaaca
     4441 tcgacattgt gccgctgtat ttcgcagccg aaagaccggt ggtttaccgg gacggcaata
     4501 tcatcatggt ggatgacgat cgtctgccgc tgaatccggg cgaaaagccg gagtacaagt
     4561 ccattcgttt ccgtaccctg ggctgctatc cgctgaccgg cggtattgaa tcaacggcta
     4621 ccacactgga tgagatcatt gacgagacac tgagttctgt ctcctcggag cgtacttcca
     4681 gagtcatcga caatgaggca gccggcagca tggaacgcag aaagcgggag ggatacttct
     4741 aatgaaaggt ttattgaaat ttattacctg cggcagcgta gacgacggaa agtccacact
     4801 gatcggacac attctatatg atgcaaagct gctgtacgca gaccaggaaa agaccctgga
     4861 actggacagc aaggtgggca gccgcagcgg tgcgatcgac tattctctgc ttttggatgg
     4921 tttgatggca gagcgggaac agggtatcac cattgacgtg gcataccgct atttcaccac
     4981 tgacaaccgc agctttatcg ttgcagatac accgggtcac gaagagtata cccgtaacat
     5041 ggcagtcggc gcatccttcg ctgacctgtc cgtcatcctg gtggacgcat cccagggcgt
     5101 tctggtacag accagacgcc atgcacggat ctgcaagctc atgggtatcc gctatttcgt
     5161 ctttgcggta aacaagatgg acttggtggg ctacagtgaa gagcgtttcc gtgagatcga
     5221 ggcacagatc gcagaactcc gcaaggaact ggatctggaa aatgtataca tcattccgct
     5281 gtctgcaaca gagggcgaca acgtgaccaa gaagtcggag aatattccgt ggtatgacgg
     5341 cgttccgctc ctggaatacc tggaaaatgt ggacattcat tcggacagcc acgaaaaggg
     5401 cttttatatg ccggtacagc gtgtgtgccg tccggaccat actttccgtg gattccaggg
     5461 tcagatcgag gctggcacga ttcacgtggg cgattccatc accacgctgc ccagcaatga
     5521 aactgctact gtcaagggca tcctcatgac ggacaagtcc gtggatgagg cattcgaggg
     5581 acagccggtc accatttcgc tggacaagga agtagacgtt tcccgtggat gcgttttcgt
     5641 caaggatgca gatgtggcag tgtataagaa actgaccgtt tctctgctgt ggatggacga
     5701 cgagccgctg agcatcggta aggactatct ggtaaaactg ggaaccaagc tgatccccgg
     5761 catcttgaca aaggtagaat atgccatcga tgtcaacacc ggtgcgcacc aggagacaga
     5821 caccctggaa aagaacggca tcgctgtctg cgagatcctg ctgacagagg gcattgtggc
     5881 agacctgttc tccaaccata aggtactggg cgaactgatc ctcattgacc gtgttaccaa
     5941 tatgacatct gcctgcggcg ttgtcgatgc ggtcagcgaa aaggtcagca gcactgccga
     6001 ccgcaattcc ttccagtacg gcgacctcaa ggcaagaggc gacatcttcg aggaattcta
     6061 ctatgatacc gagagcctga acgtgctgaa gtataacccg gtaagccgga cttatacggt
     6121 aggggatgag attcccacca gcggcgacag ctatcactat ccggacagct ttgactgcat
     6181 cgtactccgt gacggtgtgg cagtgcgcat ccgtgacaag aagatcacag ccatccttcc
     6241 ggcaagcgag tacacctatg acaacgtgcc ggtcatcaac ggccgtggct ttgaggtgct
     6301 ggttcactct gcggaagagg caaagacctt cctggaggaa tacgcacaga ccaagggtca
     6361 ggatcaggca ttcttcagca agtggatgca ctttgacacc tatcgtaagg ttgtatttcc
     6421 gcagcagtaa ttgttgtggg gtatgataaa aaagagaaaa gggctgccgg ggggatttcc
     6481 tcacggcagc ccttttttat gtgcattgtg ttgttctttg aaaaaactgg aatttacaga
     6541 tcaatcccac acaatttcca agcaatcgaa gttttctgga attgctggta cacacgaata
     6601 agcatttaat ttcgttttga ttactccatc agagcacaac catttacaat tcacaatttt
     6661 ttctaattcc gcaggcgaaa tggttgtgca aattacgatt gtatctcctg caagaactaa
     6721 ttcttctgtt ccatcgtttt ctacgatttc tgtatcatca tgcagtgcaa cacgtaccca
     6781 tgctatgttg ggcaatagct caatcttttg tagcatattc catatttcag aaagcgtcgg
     6841 acgcccaaat ccccattgat tcggagcaat ggagtcctcc gctgtatttc cattgaaaaa
     6901 atcatcgagc gttaataatg ggaggcgttc ttcatttctt tctaatattt tttttaatgt
     6961 actcaacgct atcatagtca cagttctcct atcaaatcct gacttgtttc ccttttcaaa
     7021 ctgagacaca gtgcctgcct tttcctgtta cgcttccata ttcttctgat agatcagttg
     7081 caagcctttc agcagaagca ggttgtccag tacgatcttg tgccggcagt ggggaatgat
     7141 gaactccgac agcccgccgg tggcgagaaa ggtaggtgtt tcgcccagtt cctcctcgat
     7201 gcgatccagc agcccgtcaa tgcagccggc tgcaccgtag agaataccgc ttttcatgca
     7261 ttctaccgtg ttgctgccga tgcatcgctt cggcggatcg agggaaatct tcggcagctg
     7321 tgccgtcttt tctgtcagag cattcatgga gatgcctacg cccggagcga tcatgccgcc
     7381 gatgtgccgc cgctgttcgt cgataacaga gagcgtggta gccgttccca tgtcaatgat
     7441 aacaataggc ggctggtatt cgtgaagtgc cgccacagcg tctgccacac ggtcgctgcc
     7501 cagctgtgcc ggattgtcaa tggcgatctg caaaccggtt ttgacccccg gccccaccag
     7561 aagcaccgga cagtgtgcga tcttttctac cgccttgcgc acagcgtggt tgatggaagg
     7621 gaccacagag gagatgatgc agccttcgat ctcggtcgtc tggatctgat agatctccaa
     7681 aatggttttg atggaaatgg caagctccag ggatgtggcg ttctggttcg tggagacacg
     7741 ttccacaaac aagatctgat tttcctggaa tccgccgatg acaacattgg tgttgccgat
     7801 atcaatggca agtaccataa aagaacctcc tgttttgaat tcatacgcta ttatagcaca
     7861 aatgctggaa aattgcaagc agagcatttg acttcttccg tgggagatgc tataataaat
     7921 caaaagcagt gccgcacaag cccgtctgtt ggttctgggc ggcgctgcac gaatccgata
     7981 gagaccgagg tgtgattatg cagaactttt ccacaatttt ttctgccgca gcaactgatt
     8041 ttgcgaaaac atatcccaga gacggtgcgc tgtatcagca ggcagaggaa tgcctttcgg
     8101 cgacagaacc ggaggcgctg tttgccctgg tgctgctgcg ccgggtacag cagaaacgaa
     8161 ccggcacaca ggcacccctc tgccgggaaa tgctgggttt ggagacaaaa gcggctgcgt
     8221 ttccggaact gtctcccgtg ctgcaatggt ggcaccgctg gctgggctgc ggcatttcca
     8281 tgggcgttgc cggttgggat gcgctgtggc aggcggcaga tgatgccctg cagatcctgc
     8341 cggaatcgct gacggcggct tgctgtgcgg aaatcttggg gaagttctga caggtgtccg
     8401 gaacgggaaa acaattgttt ttgtttggct tgcgtttgtt tcgtnnnnnn nnnngacagg
     8461 aactttcaaa aaacaagcag aacggaaata tagaaaaaag ttcggcgttt ctctttttta
     8521 taaaagaaaa acttctgctt ttttcttgtt tttttcggaa aaatcgaaaa aaactattga
     8581 caaaccatgc ggaatggtct ataataaaaa ctagcaaaag caaccgcaca ttaggcggtc
     8641 tgcaataggg aaagcaatgg cgctttatga agagaaatcg ggatatttct ctttgtaaag
     8701 cgcttttttg ttgtcttgca aaggagaagt gaccatggat aactacagaa accaggaacc
     8761 atttgcaaag aatggtttgt acgatcnnnn nnnnnntgta cgatcctcgc tttgagcatg
     8821 agaactgcgg tatcggcgca gttgtcaaca tggacggcac atccgaccga aaggttgtgg
     8881 acagtgcgct ccggatcgtg gaaacgctgg aacacagagc aggtaaggat gcagagggaa
     8941 agaccggcga cggcgtcggc atcctgctgc aaatttcgca cacttatttt tcaaaggtag
     9001 ccaaagaggc aggcatcccc atcggcgaca agcgggaata tggtattgca atgttcttct
     9061 ttccgcagac caaggaaaag tatgaaaagg caatgcagac ctttgaagag gtgctgaaag
     9121 aggaaaacct ggaatttctg ggctggcgtg aagtgccagt aaatccggat gttctgggta
     9181 caaaggcact gaacagtatg ccgcacatca tgcaggcatt catcaaaaag ccggaggact
     9241 gtgcaaaggg tctggaattt gaccggaagc tgtacattgc acggatggta ttcgaaaagc
     9301 gggacagcga gacctatgtg gtgtcctgct ccagccgtac cattgtatat aaaggtatgt
     9361 tcctggtaaa acagctccgt ctgttctacc acgatctgaa cagcccggat tatcattctg
     9421 caatcggtat tgtccactcc cgtttcagca ccaacaccaa ccccagctgg cagcgttctc
     9481 acccgaaccg tatgatggta cacaacggtg agatcaacac cattaccggt aacgtggata
     9541 agatgctgag ccgtgagaac attctccact gcaaggcact ggatgaccgc atgaaggata
     9601 ttttccccat gctggatgtg cgtggttctg actctgcccg tctggacaat acactggaat
     9661 ttatgaccat gtccggcatg gatctgccgc tggcagtgat gatgaccatt ccggaaccgt
     9721 ggcagcacat cccgactatg agccgggaaa agcgtgcgtt ctatcagtac tatgcaacca
     9781 tgatggaacc ctgggacggt ccggcatcca tcatcttctc cgatggtgat gtcatgggtg
     9841 ctgtcctgga cagaaacggt ctgcgtccgt cccggtatta tgtgaccgat gacggatgtc
     9901 tgatcctgtc ctcggaggtc ggcgcactgg acgatatccc ggcagaaaag atcgtgaaga
     9961 aagaacgtct ccatccgggc aagatgctgc tggtagatac cgtcaagggt aagctgatcg
    10021 acgatgagag cctgaaaaat tactatgcaa agcgtcagcc ttacggcgag tggctggatc
    10081 acaacctgtt cagcctcagc gaactgaaag tgccgaacaa gcgcccgttc cgccacggcg
    10141 gcaaggaact gctccggctg cagcgtgcgt ttggctatac ttatgaatct ctgtatcacg
    10201 tgatggtgcc gatggcactg aacggcggcg agccgactgc ggcaatgggt gcagatacac
    10261 cgatcccggc gatgtccaaa aaaaatccgc cgctgttcga ctacttcaag cagatgtttg
    10321 cacaggtaac caatccgccg atcgactcca tccgggaatc ggttatcacg gataccacgg
    10381 tttatctggg cgtttccggc aacatcctgg aagaggatga gcggaactgc cgggtactga
    10441 aagtcaacaa cccgattctg acgaacctgg atctgatgaa gattcgtggc atggacattg
    10501 acggactgaa aaccgcagat atctccatgc tgtatactaa ggatgtgtcc ctgcatgatg
    10561 ccatcgaaaa cctgtatcag caggcagaag aggcacacgc caagggtgca acgattttga
    10621 ttctgacaga ccggggcgta gaccacgatc atctggcaat cccgtctctg ctggcagtgg
    10681 ctgccctgga aacctatctg gtacgcacca gaaagaacac tgcaatttcg cttatcatcg
    10741 agaccgcaga gccgtgcgag gtgcatcatt tcgcaacgct gctgggcttt ggcgcaagcg
    10801 cagtcaatcc gtatctggca ctggattccc tgtatgatat gattaaccgt ggtctggtgg
    10861 ataaggatta ctcccaggca agcaaggcgt atattgacgc actggtttct ggtatcgtca
    10921 agatcgcatc gaaaatgggt atttccacca tccagtccta ccagggttcg aagattttcg
    10981 aggcactggg catcagcgaa agcgttatga acgaatactt cccggatact gtcagccgtg
    11041 tgggcggtat tgatctggat gatatcgcaa agcgcagcat gaccatgcac gagagtgctt
    11101 ttgactcgaa tgatctgggc atggacgagg gtctgcgcag tgtgggctgg cacaaggaac
    11161 ggagcaacca ggaagaacac ctgtacaatc cggagaccat ccacctgctc cagcaggcaa
    11221 catggcgtga tgactataac ctgttcaagc agtacacttc ttgcgtcaat gcggaggacc
    11281 ggcacatcac gctccgcagt acgctggact tcaagtttgc tgaggacggc ggcatcccca
    11341 tggaagaagt ggaaagcgtt gagtccatca tgcgccgctt taagacgggt gcgatgtcct
    11401 acggttctat ttcccaggag gcgcacgagt gcatggcaat tgccatgaac cgcatcggcg
    11461 gcaagtccaa cagcggtgag ggtggtgaag atctggaacg tatcgttacc gcaggcagcg
    11521 ctgtggacaa atgnnnnnnn nnncacaggg cgcaaagcct ggcgaaggcg gccatctgcc
    11581 ggcaaagaaa gtgtatccgt ggatcgcaaa gaccagacac tccacccccg gcgtggcact
    11641 gatctcgccg ccgccgcatc acgatatcta ctctatcgag gatctggcgc agctgatcta
    11701 cgacctgaaa aatgccaata agaaggctcg catctctgta aagctggttt ctgaggcagg
    11761 cgttggcacc attgcagccg gcgttgcaaa ggctggcgca ggtgttatcc tgatctctgg
    11821 ttatgacggc ggtaccggcg cagcaccggg cagctccatc cataatgccg gtctgccgtg
    11881 ggaactgggt ctggcggaaa cccatcagac actgattcag aacaacctgc gttccaaggt
    11941 cgtgatcgag gcggacggca agatgatgag cggcagagat gtggtcattg ccgcaatgct
    12001 gggtgcagag gaatacggct ttgcaacagc gccgctggta accatgggat gcgttatgat
    12061 gcgtgtctgt aacctggata cctgtccggt aggcgttgcc acacagaatc cggaactgcg
    12121 gaaacggttc cacggcaagc cggaatacgt catcaacttt atgcgcttta tggcacagga
    12181 aatgcgggaa tatatggcga aactgggcat ccataccgtg gatgaactgg taggccgttc
    12241 tgatctgctg gtacagaagc agtatccggc aggcacacat gagagcaaga tcgatatgtc
    12301 caggattctg accaatccgt atgccaagga ggacagccac gtcaagatgc acttcgtgcc
    12361 ggaggatgtt tacgatttca agctggataa gaccattgat gaaaccgtta tcattccgga
    12421 gatgaaagag gctctggcga agaagcagaa aaaggagatc agcatcaatg tcagcaactt
    12481 aaaccgttcg ttgggtacgc tgtttggtgc ggagatcact cggaaatatt acaacaccct
    12541 ggacgatgat acctttgtgg tacattgtaa cggtgcaggc ggtcagagct ttggtgcatt
    12601 tattccgaag ggactgaccc tggcgctgga gggcgacagc aacgactatt tcggaaaggg
    12661 cttgtccggc ggtaagctgg cagtgttccc gccgaaggga tcgaagttca aggaagacga
    12721 aaacatcatc atcggtaacg tagcgctgtt cggcgcaacc agcggcaagg cgtttattaa
    12781 cggagtagcc ggcgagcgtt tctgcgtccg gaactccggc gcatcggttg tcgtagaagg
    12841 cgtgggcgaa cacggctgcg aatacatgac cggcggctgc gcagttatcc tgggtgaagt
    12901 gggcaagaac tttgccgcag gtatgagcgg cggtattgcc tatgtactgg acagagaaaa
    12961 tgacttgtac accaagctga acaaatctct ggtcggcttt ggcgcagtga ccaagccgga
    13021 ggatgtggca cagctcaagg gcttgatcga agaacatgtg gcatggacag attccaagct
    13081 gggcaagaag atcctcaaga atttcgatac ctgtctgccg catttcaaga agatcgtgcc
    13141 gcatgattac gcaaagatgc tggagagcat tgcggaattt gaggcaaagg gcatgaaccg
    13201 ggaagaggca cagatcgaag cattctatgc caacacgaga aattaacagg agggactatc
    13261 atgggaaaag caactggatt tctcgaatat gagagagtcg aaaatgccgg acaggagccg
    13321 ctggagcgaa tcaagaccta tcgtgaattt cacacgccgc tttcggacga agaacgccgc
    13381 aaacagggcg cacgctgcat ggactgcggt gtgccgttct gccaggcagg caagccgatc
    13441 tttggcatgg tctccggatg tccgctgaac aatctctgcc cggagtggaa cgacatggtt
    13501 tacaacgggt tctgggacga ggcggcagac cgtctgctgc tcaccaactg tttcccggag
    13561 ttcacctccc gtgtctgccc ggcgctgtgt gaaaaagcct gcacctgcgg actgaacggc
    13621 gatccggtca cagtcaagga aaatgaatat gccatcattg aaaaggcatt tgcaagcggt
    13681 catatcaagg cgaatccgcc gaaagtccgc accggaaagc gtgtggcaat tgtcggctcc
    13741 ggtccgtctg gtctggcggc agctttgcag ctgaacaagc gtggacatca tgtcaccgtg
    13801 ttcgaacggg aagaccgtgt gggcggtctg ctgatgtacg gcatccccaa tatgaaactg
    13861 gaaaagacag tggtacagcg ccgtgtggat atgatgcagg aagagggcgt agagttccgc
    13921 accagcgtag atgtgggcaa ggatatcacc gcagagcagc tgctcaagga ttacgacgct
    13981 gtgatcctgg cgtgcggttc gtccaatccg agaaatatca acgctgaggg acgggatgca
    14041 gacggcatct actatgcggt ggattttctg aaagccacta caaagagcct tatcagctcc
    14101 aatctgtccg atggttacta tatcagcgcc aagtataaaa atgttatcgt catcggcggc
    14161 ggcgacaccg gcaacgactg cgtcggcact gccatccgcc acggctgcaa atccgtggta
    14221 cagctggaaa tgatgccgaa gctgccggat acccgtgcgg aaagcaatcc atggccggag
    14281 tggcctcgtg tctgcaagac ggactacggc caggaagagg caattgctgt attcggtcac
    14341 gatccccgta tctatcagac aacggtaaag cggtttatca aggatgacaa gaaccacgtc
    14401 cgtgcagtag agaccatcaa gctccagtcg gtgcatgatc ccaagaccgg cagaatgcgg
    14461 atggaggaga ttccgggcag cgagcagatt ctggacgctg atctggtgct gatcgccgca
    14521 ggtttcctgg gcgcacaggc gtatgtcaca gacgcattcg gcgtggagat gacaccgaga
    14581 accaatgttt ccacaaagat cggacactac agcaccagcg tggataaagt gttcaccgca
    14641 ggcgatatgc accgtggaca gtctctggta gtctgggcga tccgtgaggg cagagaggct
    14701 gccaaggaag tagaccagta tcttatgggc tataccaatc tgaattgatg ttatcattct
    14761 gacaatttca agttattcat ataccctttg caagaacagg ctgcgtttcc ggagacggaa
    14821 atgcagtctg tttttcttgc tttttgccgg gacgtgtgtt ataatatagg aaatataccg
    14881 ggtgatcgga aaaaacagtg gagttataac agatgaaaaa atacaaaatt gcagccattt
    14941 tgatgataat acacggggga ttcatggaaa tcggcggtgt cttgtgtatg atacctgcgc
    15001 tccttctggg aacggataag tttgatatcg ggcaatattt ttcgtttaag ctttcctatt
    15061 ttcaggaaaa tctttccttg atgatagtaa tgggcgcaat gtacggcgta cttcggctga
    15121 tcggggcaat tggcttgctg aaaaacagaa tgtggggact ggtgctggct gttatcacct
    15181 gtgttattac actgctgttg atgatgttcc tgctgcctgc cggaatcatg gacggcatac
    15241 ttgcaggcag tgttttgatc ttgctgctga cacagtattt cggcgataaa aaaatagggg
    15301 atgagaaatt atgattacca ttaccccatt tgaagaatcc tacactcagg atgtgataga
    15361 cctggtgctg cactttcaga atgacggcac tcgtcctgtc gtcacagtag cagatcagcc
    15421 ggatctgctg cacatcacag atgcctattt tcgcattggc ggcagtttct ggatcgccag
    15481 agacggagaa aagcttgccg gtacgattgg cattatgccc tgcggaaacg gcgtcgctgt
    15541 gctgaaaaag tttttcgtct atgagaaata ccagggcgag ccggtacatc tgggacggca
    15601 gctttatgcg gtactgctgc aatttgcaaa agagaacggc atccatacat tgatgctgga
    15661 cacgccgcac aataccgtca gggcacatcg gttctacgag aaagccggat tccggaaagt
    15721 atcagaaaat gaactgccgg tgcggttcag ccatccctat gcggactgcg atttcttttt
    15781 gctggaatta tagcgtactt gaagaaagga tagatatgat gcagacagaa accaggtaca
    15841 tccgtccggc agctgagaga ggacgggtgc aaccttatgc gaattgattt taatgaaaaa
    15901 gaatctgtca ccgttcccgg catgaatcac ggcaccggaa tgatgacagc aaaaatgtac
    15961 gcagatgtgc tgggaaaagt gatttcctgt gccattcatc ctggcggctc catcgggatg
    16021 caccgccatg aaacgagcga tgacatcaac tatgtgattt ccgggaaagg cattgcagta
    16081 acagacggcg cagaagaacc actgtctgcc ggagtctgtc atatctgcaa aaaaggttcg
    16141 gcgcacagca tcacaaatac cggcacagag gatctggtgc tgctgactgt tgtggtggaa
    16201 cggtaaaacg tcagtttggg agaggagaaa cacacatgac aaaaaaagaa cgtgcattgc
    16261 ttgcagtgca gacgctggaa gaggtttatc cccaggcgac ctgtgcgctg caatatgaaa
    16321 agccctatga gctgctgatc gccgtgcggc tttcggcaca gtgtaccgac gcaagggtca
    16381 accagatcac gccaaagctg tttgcggcgt atcccacgct ggaatctttg gcggcggcgg
    16441 atattgccga cctggaagat atcgtccgtc cctgcggctt ttttcacatg aaagccaaga
    16501 gcatcaagga gatgtctgca cagctgatac tgaactttgg cggcgttgtg ccggacaata
    16561 tggaagatct gctgtccctg tccggcgtgg gacgcaaaac agcaaatctg atcctgggag
    16621 acgtttacgg caagccggca atcgtggcgg atacccactt tatccgcatt accgggcggc
    16681 tggggctgac caagtccaaa gatcccgtac aggtggagaa agacctccgg ccgctgatcc
    16741 cgccggagcg ctcttcggac ttctgccacc gtgtggtatg gttcggcaga gacacctgcc
    16801 gtgccagaaa accgcagtgc aagggatgtc ccatggcggc attctgcccg tcacggagcg
    16861 aataagcgag cggttcatcg gcactggaca ggggaagtgt tgcggaatgc acagagaatc
    16921 tgcttgaaaa tgaattgtgc tttgtgaaaa tatttcaaaa tctattgaca agcaggaacg
    16981 ttcgtgctat actagttatt gtataaattg agtaaaaagg cggtaaatcc atgggcattt
    17041 ttagtaaggt attcggttca tacagcactc gtgaactggc acgtattgaa ccgttgaaaa
    17101 agaaggttct ggaactggac gaggagtttc aggcactctc tgacgcagat ctgaaagaaa
    17161 aaaccaatga gttccgggaa cgtctggaga acggcgaatc tctggaaagc atggaagcag
    17221 aggcactggc gacggttcgt gaggcagctt atcgtgtcct gggcaaaaag ccgttcccgg
    17281 tacagatcat tggtgcaatc gttctgcacc agggccgtat tgccgagatg aaaaccggtg
    17341 aaggtaaaac actggtggct tgcgttgctg catatctgaa tgcactgccg ggcaagggcg
    17401 tacacgtggt taccgtcaac gactatctgg caaagacaca gtcggatgaa atgggcaagg
    17461 tgctgcggtt cctgggtctg acagtcggct gtatcctgca tggtctgaac aacgaccagc
    17521 gccgtgcggc ttataactgc gatgttacat acggcaccaa caatgagctg ggatttgact
    17581 atctgcgtga caacatggtc atttataaga aggacaaggt acagcgggaa cataactttg
    17641 ccgttgtgga cgaagtggac tccatcttga tcgacgaggc gagaacaccg cttatcattt
    17701 ccggtaaggg cgacaagtcc actgcgctgt acggcgttgt ggataagttc gcaaagacgc
    17761 tgactgcggc aacggtcgta gaaatggacg acaagcagga ccaggaagag atcaacgaga
    17821 atgcggatta catcgtagac gaaaagtcca agacagcgac gatcacacgc cgtggtgtca
    17881 agaaagcaga agagtacttc gctgtggaca atctgatgga tccggaaaac atgacgctgc
    17941 tgcaccatgt nnnnnnnnnn atgtcaacca ggcactgaaa gcaaacggcg tgatgcggaa
    18001 ggatatcgac tatatcgtcg aggacggcga agttgtcatc atcgacgagt ttaccggacg
    18061 taagatgctg ggacgtcgtt tcaatgacgg tctgcaccag gcaatcgagg caaaggaagg
    18121 cgtaaaggtc gcacaggaat ccaagaccct ggcaaccatc acattccaga actatttccg
    18181 tctgtacacc aagctgtctg gcatgaccgg tacggcaatg acagaggaag acgaattccg
    18241 tgaaatttac aagctggacg ttatcgcaat tccgaccaac cgtccggtac agcgtatcga
    18301 ccacacagac ttgatctacc gcacagaaaa gggcaagtac aaggcaatca ttgaacagat
    18361 cgccgagtgt cacgaaaagg gacagccggt tttggtcggt acgatttcca ttgaaaaatc
    18421 cgagctgctg tctagtatgc tgaaacgcaa gggcatcaag catgaagtgc tgaacgnnnn
    18481 nnnnnngctg aacgcaaagt atcacgcaaa ggaagcagaa attgttgcac aggctggtaa
    18541 gctgggtgcg gttactatcg caaccaacat ggctggccgt ggtacggata tcatgctggg
    18601 cggtaatgcg gagttcctgg caaaggcaga aatgcgcaag cgggaatatc cggaggagat
    18661 catcactgag gcaatcggct atgcagatac cgataaccag gaggttttgg atgcgagagc
    18721 cgtataccag gaactgtaca agaagttcgg cgatgaggta aaggaaaagg caaaggatgt
    18781 taaggcagcc ggcggtctgt acattctggg tacagagcgt cacgaatccc gccgtatcga
    18841 caaccagctc cgtggtcgtt ccggtcgtca gggtgacgag ggcgaaagcc gtttcttcct
    18901 gtcggtagaa gatgatctga tgcgtatttt cgcaggcgac cgtctggaaa acatgatgcg
    18961 gacactgaac gtggacgaag atacccccat tgaaagcaag gcactgacca agatcatcga
    19021 gtcctctcag cgcaaggtag agggacggaa cttcaacatc cgtaagaatg tcctgaacta
    19081 cgacgatgtt atgaacacac agcgtgagat catttacaag cagcgtgcgc aggtactgga
    19141 cggcgaggat atccacgaca gcatcatcaa gatgttcgag gaactgatcg caggtacggt
    19201 aaagcagtac atcaacgagg aagaaaccga tcacaaccag tggaatctgg ttggcctgcg
    19261 ggatcacttc ctgggctgga tcaccgaaga ggacgatctg gaatatgatg aggcagatct
    19321 ggcaggtttg accactgcgg atatcaccga ggcgctgacc gaaaaggcaa aggcactcta
    19381 tgctgccaag gaagaagagt acggcgcaga tatcatgcgt gagctggaac gtgttgttct
    19441 gctgaaggta gtcgatacca agtggatggc gcatatcgac gatatggacg aactgaaaaa
    19501 gggtattggt ctgcgtgctt atggtcagca gaacccggtt gtcgaatatc gttatgaagg
    19561 cttcgagatg ttcgatgcaa tggtagactc catccgtgag gatactgtcc ggatgctgct
    19621 gacagtacgt ctccagaaga accaggctcc ggaacgggag caggtttcca agccggacgc
    19681 accgaatgca ggcgcaggtg acggcagctt tggcgcacag cgccgcagcg caaagaagaa
    19741 gtaaatattc gttctgtgat ttgaatgatt cgaaaaacgg gggacatggc tttcgctgtg
    19801 tacccttgtt ttttacagag gaggtgcagc catgcaggaa acaggctatg aaacattttc
    19861 gttctattat gatgcactga cagaaaacgt ggattatccg gcgagagcag catatctgga
    19921 cggactggtg cagaaatacc tgcgctccgg cggaaaggtc atgctggatc tggcgtgcgg
    19981 tacgggaaca cttaccgagg aaatggcgaa acgtggatat gacatgatcg gcgttgacta
    20041 ttccgaggga atgctgggac aggcaatgga gaaaaagatc gagcagcagc tgccgatcca
    20101 atatgtctgc caggatatgc gggaactgga actttacagt accgtggatg caaccatttg
    20161 cacgctggac agtctgaacc atcttcccgg tttcgaggat gtggagactg tgttccggaa
    20221 ggtctgggaa ttcacagagc cgggcggtgt ttttcttttc gatatgaata cgctatataa
    20281 acaccgggaa ctgctgggta gccaggtgta tgtttacgaa acggacgagg tctactgcgt
    20341 ctgggaaaat gcgctctgtg aggatgactg taccgtcgag atcacgttag atctgttcca
    20401 gctgtgttgt gaggacggcc gctatcgcag aacgcaggag cagatcacag agcgtgccta
    20461 tgtgccggag caggtgacgg catctctgga gaaaatcgga tttcatgtgc tgggtgtgta
    20521 tcatgcggac accgaagagc cgctgcgtcc ggacagtgaa cgtatgatcg tgattgcaag
    20581 aaaggaagcg taaacaatgg gaattttgaa acgtgcgata gcaaaggatg cttcggttgt
    20641 ggcaagcgct gtggatgcca ccgatattgt ggctgagatc gagcgaatcc ataaaacatc
    20701 ggcagttgtc acagcggcgc tgggacggtt gtccattnnn nnnnnnngca gcctgcatga
    20761 tgggctatga cttgaaaaat gaaaaggata cagtgacgct ccgcgtaaaa ggcggcggtc
    20821 ctgccggaat gctggtcgcc gtggcagaca gtcacggcaa tgtaaaagcg gacgtggatc
    20881 agccggttgt ggaacttccg ctgaatgcga aaggaaagct ggatgtcggc ggcgcagtgg
    20941 gaacagacgg tacactttct gttgtgaaag atctgggcat gaaagaaccc tatgtgggcg
    21001 ttgttccgct ggtttccggc gagattgcag aggacgtggc gcagtacttc gctaccagtg
    21061 aacagacgcc gacagtctgc ggtctgggtg tgctggtgaa tccggatctg acagtcaatg
    21121 ttgccggcgg attcctcatt gaggtgctgc cattcgcaag tgatgcctgc atcgatacca
    21181 ttgagaataa tctgaaaaat ctgccgccgg taacgacaat gatgtctcag ggcatgacac
    21241 cggatcagat cgcactgcgt ctgctggatg gtctggaacc gaatctgctg gacgagggcg
    21301 aagtgaaata tcagtgcgat tgcagccggg agcgaaccga gcgggtactg aaagcgctgg
    21361 gaaatacgga gctttccaag ctggcagaag agggcgaacc gatcacggtg aactgccatt
    21421 tctgtggcaa ggaatacaag ttcacgccgg atgagctgca aaagctttgc agcgaagagg
    21481 aagaataacc ggacaggagg ggattcgata ccatgcgaaa ttggaaaaag attgtgggaa
    21541 tgctgctgat tatggcggtc tggctcggtc tgatgtggcg gctttccaca gaaaatggaa
    21601 cggagaccct caaagacagt atgcgctttg cccggaaatt tggcgcctgg atctacggtg
    21661 agccgactgc ggcacagctg gatcatctga atctggttct gcgcaagatg gcgcacgtgt
    21721 tcctgtatgc cgtctttggc ggaatgcttg cggtattctg gcagcttttg ctgcggcata
    21781 aggtctggcg cattctgggt gccatcggat gcagcacagc catagcattt ctggatgaac
    21841 tgcaaaagat tcccattgcc ggacggcact ttgacgcctc ggaatccatg cttaatgcgg
    21901 tcagcgccgc tgtggtgatt ctgatttatt tcggtattgt ccggtgtgtt tcccgttgga
    21961 gacaaaatcg gcaggcggca aaaatacagt gaaatacagc acacgctttt ctggtattcc
    22021 ggaaagtgtg tgcttttttc ttgtgaaaat tttcaaaata tacttgattt tcaaccggct
    22081 attttgtata atataatgga aagaaaatgg gtatattgtc gttttgccaa tgtgctttga
    22141 agtgaaagga atgatgcaga atggagacgc aggaggcagg gctgaaaact gtaaataata
    22201 cttatatgga gtcgcatcgg atcttgcgta ccccttcggc tttggtaagg gatgcgctgc
    22261 tttacatcca ggaaatcggc gaaatggttt gcaaagagcc acgccagaac tggcggaatt
    22321 ccctggactc ctatttgctg attctggtaa aagagggctg cggtacgatc acagttgggg
    22381 aagagacgct tgccatgcag gctggcgacg tggctttttt ggattgtcac aatccctatt
    22441 cccactgctg tgatgcaaga gacccctggc atattctgtg ggtgcattgt aacggtgctg
    22501 caatgccgca tctgtacgat attttccgtc agcgcaatgc gtcttttgtg atgagcggag
    22561 cggcagagtg gatcgaaccg gttttgcaga acctggagcg aattgccatg gcagaagagc
    22621 cggattatga actgtatgaa tcggagtgtc tgacgcagct gataacattg attctgacca
    22681 gaaaagcaga aaagacaggg catatttccg gggacacttc gcagaaatgg gagcagattc
    22741 atcagtatct ggaggaacac tttgcagaga agatcacgct ggaggaactg ggaaagcgtt
    22801 tcggcatcag caaatactat atgctccgtg ggttcaagag aaaatacggc gtgacaattg
    22861 tgcagtttct caatcagtgc cgtatgaact acgccaagaa cgaactgcgg tttacggaca
    22921 agcagatcga caccatcgca gaagaatgcg gcattcatga ttccagttat tttaaccgca
    22981 ttttccgcac cacagagggt atttccgctg gtgcataccg gcggcagtgg cgaaattgaa
    23041 gtaagaatct gtcttcacaa gcatatacac gtgttttttc ggcaaatttt gtaaaaaata
    23101 cttnnnnnnn nnngaagaca gcctggaaaa atgattcggc tgtctgcttt tttgtttatg
    23161 accaaaagaa aaggactgct ccggtgtggg agcagccctt ttcctttata ataggagtgt
    23221 agaaaatatc agaatgtcgt ttactgcggc gttaccggca gagagctgat gatgtgtacc
    23281 aggaactgca gcagggacag cgcatcgtca ctggataata cattgtcagc catgcagtct
    23341 gcatttgctc ttgcctgtgc agaaagctct acctgtccag ccgctgcctt gttcatcagg
    23401 acggcatcgg tgatgtccac tctgccgtcc aggtttgcgt caccatacag ggaaaccgtt
    23461 gccggctcag tttctgttgt tgcagaagat tctgttgtga caatgcccgg agcctctgtg
    23521 ccagaggatg cagttgttgt cgttgttgca gaggtctctg tgctggtttc actggagtct
    23581 cctgtcacag ttgtggtcac ggattctgtt gcagaagttt ctgtagcgga tactgtggtc
    23641 tctgtttcag agacggttgt ttctgtttcg gaatctgtgg tttgagaagc atcgtcagaa
    23701 gttgttgtgc ctgttggctc agtggtagtt tcgccgcttt ttggaagata tgctcctgtc
    23761 agcttgacgc tggaggtttc cattgcatcg ccggcgccgt catttgccca ccagatctgt
    23821 acctgtgcat tcttctgctt gattgcctcg tcagagatct tcttaatcat gtcagcgtcg
    23881 gtcacttcgc tcttgccgtt gttataagtg acattaaacg gttcatccag ctttgcagtg
    23941 acttcaccct tggaatcaac ttcccacttg tagttcagcc agtagtctac gccgtctact
    24001 gtagcagaaa tgccgataca gccgtttgca taattgacat cgccgtcaaa atcaaatgtc
    24061 agatcaacac gattgccgat agcctgcttg aacgaaatgt tgtatacgcc gttgtcagcc
    24121 acgatcttag cagcctcgcc cttgccttcc ggtgtggtgg tagtggtggt tgcagtggtg
    24181 gtgtggctcg gctttgtgcc ggtgccctgc tgtccgtttc catacagatc atccggaagt
    24241 tcatccagtg taatgcagta gtcgctggtg tacatttcct tcagatcttc cttggtgttg
    24301 aacagttcgt tggtcaggaa gttggtgtcg ttgctgccgc cgtcatacca cgggcagaaa
    24361 tacagccagc cagccttttc ggttaccagg ttgttcagcg tcgggatgct gtcattttca
    24421 gccatagcga ccatcttctt gctgttatac ttctgcatga tggaatagaa ggtgctgctg
    24481 atcgcactgt cgttgtgctt cagtacagcg ctgccggtag accagtctgt gcagttgtac
    24541 ttgtcgtatg ctacaatgtc tacatattca tcgcccggat accagtttgc agaggtatca
    24601 tatgcgtagc tgttccattc ccagatcaga ttgtgcagac cgtattcctc ggtcagtgtc
    24661 tcataggtga ggatccacag ttccttataa acggcagagc cttccttgcc ccaccagaac
    24721 caggaaccgg tctcaccgcc gccgccttct gcctcatgga gcggacggaa gatcagcgga
    24781 acatctgcat cctgcagttt ctgcagctca gcagccagtc ccttcaggca aagcatataa
    24841 tattcccgtt ccttggagcc ttctaccaga atcttggacg gttcgaagtc agtctccttc
    24901 ggaacatatg ttgcatttgc ccagtctacc ttatcaccga tattgtagct ggagaagttc
    24961 tttggtacag tgatatgcca ggatgcagtt gcaataccct ggttctcgga gtacggattg
    25021 tcattgaccc actggatgat acgatctgtg gtgccgtcct cgctgccgta cagcgggttg
    25081 cagttcagat agtcgaagcc gcggattgcc gggtatttgc cggtggtgtc gtggatgtag
    25141 ttgaactcat attcaaagtc atgaggacca tacttataga tctcctgctg tccggagatg
    25201 atgtgcttgc cgtaaacgct ggacagatac tgcatcaggc tgtctgcctg tgcggttgct
    25261 gccgggtcgc tgcacttggt gtcgctggcg gtgatgtccg ggagtgttgc ctcttctact
    25321 gtgatgtagt cgaagttggt ccagccccag ctgcccacga tggaaatctt gttgtcgccg
    25381 gcatccagtt ttacagaacc cagatccagt tccttccact cgccttcctt ggtgggggag
    25441 aaactgcact gaccctggtc tacgtcgttg acgtacagat tctggatctt gttgccgtac
    25501 ggtgcggaat aggctacctt aatggtatac attcctgtct tttctgtggg aacggtaaca
    25561 gaaatctcgt cgccgccgtt ttccagaaat acatactttc cgccggatgc gtttgcatca
    25621 tcgtccttga cggaattttt cgcagactgc ttgccatcct caaattcata tttggtggct
    25681 gctgcacctg cctgcatcac actcgccagt gccatgcagt ttacggccat aacgcctgct
    25741 acaacagcag tcaatgtccg cttgagattc ttcttcatag tctaattctc cattcttttg
    25801 cctgccggac gatgcaaccg gagtcgccgc agacaatgat tcaaaaattc aaatgataaa
    25861 aagaagcaca atgctgtcag ctgcacacag cattcacggt tatttctaag tataccacac
    25921 tccttgtaca atgtcaatag atttttctgc acatttgcag aaaatttgat aaaaggcaca
    25981 tctttggacg catatggagt tcgctttgaa aaaagtatgt gcagctgtgt aaaaagctgg
    26041 ttaaaaataa ggcacaggag agaaaaagga ttgcattttc ggcgatgctg tggtatacta
    26101 gtgacagaaa ggcgaaggtt taccggaata cacggaaagg cagaggtgaa actgttatga
    26161 tgcggcatag gctacagcat tgggagcagc agacactgct ctggtttcag gaacatttgc
    26221 ggcgcagctg gctgacagcg gctatgaaga tcgccacgtt tctgggaaac ggcggcattc
    26281 tctggctgac ggcttgcgcc tgtctgctgg ttcgccagca gaccagacga gcggcgctga
    26341 cggcactttt gtcgctggtg ttttctgcgc tggtctgcaa tgcgctgctg aaaaatctgg
    26401 tggagcgtgc gagacctttt gacaaaattc ccggcttgca gtttctgatt cgaaagcccc
    26461 acgatttttc gtttccctcc ggtcacactt ccagttcttt tgcggtagcg accgtgttcc
    26521 ttgccacgct gccgctctgg tttgggctga ctgcgctggg aatcgcagcg gtgatcgctt
    26581 tttcccggat gtatctgggc gtgcattacc cgtcggatgt gctttgcggt atggtgcttg
    26641 ggattctgtt tggattggcg gcgctgatcg tgctgccgct gctgctggaa attccctggc
    26701 tgcccgaaac cgttcgtgcc tgggtcggca cgggaatggt ttctgcaaat ctgtaaagac
    26761 agcatataaa aaagccgatg ttcatttgaa acatcggctt ttgtggttta ctatgaaaat
    26821 gagcgttact ctgcgctgag attttccagg tctttctgcc acagcgtcac gcaggcatcg
    26881 ctgggcattc tccagtcgcc tctgggcgag agagtcacgc tgcccacctt gggaccgtcc
    26941 gggagacagc tgcgcttgaa ctgctgggag aaaaagcgcc agtaaaactt tttcagccac
    27001 ttcaaaatcg tggcgttgtc atatgttccg gcaaacgcca gctttgccag ccggtaaatt
    27061 ttatggggcg caaatcccag acgcagcacg tagtacagga aaaagtcgtg gagcgcatac
    27121 ggacctacca gttcttccgt tttttgtgca atggtgccgt cctgttccgg cggcagcagt
    27181 tccgggctga ccggtgtgtc caatacatcc cggaggacag cactcagtgc cggctcggtc
    27241 tgggatgcct catgcgccac cagatagcgc accagcgttt tcgggataga ggcgtttaca
    27301 gcatacatac tcatgtgatc gccgttatag gttgcccagc ccaacgccag ttcggacagg
    27361 tcgcctgtgc cgatgataat gccgccggtc tggttcgcca gatccatgag aacctgtgtc
    27421 cgttcccgtg cctggctgtt ctcgtatgtc acatcgtgaa cggattcgtc atgcccgatg
    27481 tcccggaagt gctgacggac gctgtcctgg atggggattt cccgcagggt cgcaccatat
    27541 gcctttgcca gacggcaggc gttctgatag gtgcggtctg ttgtgccgaa acagggcata
    27601 gtgactgcca gcatcccggc agggtcaagc tgacaaatgc ggaaagcgtg taccataacg
    27661 ataagtgcca gcgtagagtc cagtccgccg gagagtccga ccacagcggt tttgcagccg
    27721 atgtgccgca gacgggtggc gagtcccatt gcctggagcg tcaggatctt ttcgcagcgc
    27781 tgtgtccgct ctgcagtatt gctcggcaca aacggcgcag catccacaaa gcggtcgagc
    27841 cgggattctg tcagttccat ggagaagggg acagccggac ttgccggtgc aatgggctgg
    27901 aacgtatttg agcgccgacg ctcttctgcc aggcgctgta cgtcgatatc gccgtagagg
    27961 atgccccggg caaacagctt ggattctgcc aggatcgagc cgttttccag aatgaggtcg
    28021 tgtcctgcaa ataccatgtc ctgggtggat tcgccgaatc cggcatcggc ataggcatag
    28081 gcgcagagca ggctgccgga ttttgccttg aggatggtac ggcggtagtc ctccttgccg
    28141 atgatctcat cgctgcacga cagatttacg atcagcgccg caccggatct tgccatggtg
    28201 acagacgggg tgtccgctac ccacacatcc tcgcagatct ccacgccgaa agtgaagtcc
    28261 ggcatttccg cacaggaaaa gagcagcttt gcgccaaacg gaaacgcata ttccggatgg
    28321 ctgcggagtg tgcgcatctc tgtcggcgtt ccggcatccg gtcccggcgt gaaatggcgt
    28381 gcctcgtaaa attcgctgta gttgggcaga ttctgcttgg gtaccatgcc cagcatcttt
    28441 ccttgacagc agacggcggc gcagttgtac aatgcgccgt tggatgcgta gggaagtccc
    28501 acgatgacca gtatggacag gtcagccgtt tcgttcagaa tgcgaaccag ttcttcctct
    28561 gcgccccgga gcagggtctc ctggagaaac aggtcgccgc aggtgtaccc ggtcagacaa
    28621 agttccggga aagcggcaag ctttgcgccg gcttgtgccg cctctttggc ttttgcgatg
    28681 atctggtcgg cgttgtatgt gcagtctgcc acacgcagcg gcggtgtgac gcaggcaatt
    28741 ttcagaaatc catctttcat ggtgtttgtc tccttatcgg ctatagtact tttattatac
    28801 cccaatgttg gaaaaattgc aagtgggaga ctgccgttgt ttttcagaac aaaaagcagc
    28861 atgacaaaag tctgtcatgc tgctttttct cagatggtaa tgaattcgct gctggcatag
    28921 cccaccatgc cctggtagga taccacatcc cagccgttgg aacggctgta gaccgtgagc
    28981 acgctgccgt tggggattgt gccgatgatc gctccctggg tagaaggaaa ctgccgtaga
    29041 ttcagattgc tcccgtccgt gacaaccgtt ccccggaaaa cagggccagc ctcgatcagc
    29101 ggaatgccga agtagtcaca aagggactgg gtcagattga cggcaattgc ggtgaggtta
    29161 ttgcggatcc agtctgcatc ttcccggttg tcatggtagc cgatctcaca gagaatcgcc
    29221 ggcgcacgtg tctgcgtcac ttcgcccagg gtagttgtgg gtttggcaga gcttttgtcc
    29281 ggcaggggat agatgctcat gaaattgttt gccgtaatca ccgccagcgt ttcgctgtac
    29341 ttgtcatagg gggaatagta cacgtcaatg ccacgcagct ttccggcgag agattccggc
    29401 gctgcgttgc tgtggagcgc cagatgcacg tcaaattcgc cggcgttgga gtcggcaatc
    29461 gctccggtca catttcggtc ggggtcgttt cggacgtagg tgatgccgct ggcacgcaga
    29521 tacggttcca tccggtcggc aatcagattc atgtactgtt cttcgttgcc gccgtcaaca
    29581 taggggttcc attcctgcgt ggatgggctg agaaatactt taggcatatg atttcctctt
    29641 tcctttccaa agtcttaggg caataccgca caaagcttgt gcgtaagatg cgtagctgga
    29701 tttttcggca gattgcatag aaattccgca ggcatactgt cgtatggcaa gggatttctg
    29761 ggcaatatga cggaaaatca gcaagcagat tgcgtgcaaa gccgccaggc gggctttgtg
    29821 cggtattgcc ttagcaagcc gtagcctgca aagcgtttct gctcgttttt actgtatgca
    29881 ggatacggaa aaatggtgca gtcagccgac agaggaatca aaaatggttt cgatgatgcg
    29941 tggcgaatgc tccggcggat accgccactg gcgcagatga ttctggctct tcggaataaa
    30001 atagtgcagc ggctgcggcg gttcgctctg ccggaatgtg tcgatcagaa tgagcttgac
    30061 tttcattttc tccggcagga tcgctttgat gtacacgctg gcgcacattc cggcggcagc
    30121 gtcctcgtgg ggttctgcca gcccggcgag gttcggggaa agctctacaa atacgccata
    30181 gctttccaca gaacggatga tgccggcgac ggtctgcccg gcggagaaca tggcggcgtt
    30241 ttctgcccaa gtgccgagca gttcccggtg cgtcagatga atgcgtcccg gttccacctg
    30301 gcgcacgatt acccggatgg actgtccgac ggtgaaacgg tcacggggat gggcgatacg
    30361 ggagacggag atggtatcga tggggagcag ggacgggatg ccgcagccga tgtccacaaa
    30421 tgcgccgaat ttttccaggt gcgtgacaac tgccggcagg atgtcacccg gcgtacagtg
    30481 ggagagatac tgcttccagc attcctcctg gacgatgcgc cgggagagga ttgccaccgg
    30541 cagaccggat gcgtcctctt ctatgcgcag gatgcggaaa cagaccggtt ttccggcacg
    30601 tgcgatcagt gcgatgtctt ttgtcgtgcc ttctgcgatg cccattgcgc cctcttcccg
    30661 tgggatgatg ccacgcatac acggcaggga aacggcgaga ttatggctgc tgtcacagag
    30721 gacggcacga gcctccagga tcatgccggt gtgcatggcg tgttccatgg cggatatgct
    30781 gcgcagtgca acttgatttt ccggcgtatg cagccggttg ccttctggtg catagatcat
    30841 tttttgacct cccgataatt ttgaagtgag ctgtttcggc gtctgaatac cctctgaaac
    30901 agacgctgtg tgcattcagc ggaatctccg tgtcggatgc tgtttcagtg tatgtatgag
    30961 aatacgggaa tatgcggaca gcattcgaat gaaacgtgaa aaaactggaa atcaaaaagc
    31021 ggcttttatg aaagttccac ggatttcatc gtcggatttt atagcatttc cacaaagaga
    31081 aaaaaatatc ttctgaaaat attgccttat tccgagaaat ggggtataat ataatgtact
    31141 tatgatgcaa gttgtgcgca aaaacgggag ggctgaacat ggatgtgcgt ccggcagata
    31201 ttctggtgat gaaaaagccg cacccctgcg gcagtcagac catgtatgtg attcgggcag
    31261 ggatggattt caagctgcgc tgtaccggct gcggtaggga gtttatggtt cctcgcaata
    31321 agatcgaacg gcgcatcaag cagatccggc gggatgcccc ggaatcagaa gagtgataca
    31381 gacagaaagg caggaacgca gatatgctgg ataagcttcg gcaaatggaa gaaaactacc
    31441 acgccatgca ggagaaaatg atggatccgg acgtggtggg agacaatcag cagtatgcgc
    31501 agctgatgcg ggaatacaag catatgcagc cgatcatgga gaagtatcac gaatatttgc
    31561 aggcacagcg tgactttaca gaggcaaagg aactgctgga cagcgagacc gacccggaac
    31621 tccgggagat ggcacaggca gaactggaac agagccgtgc ggcaatggag accatgcagg
    31681 aagaactgaa acgcctgctg ctgcccaagg accccaacga cgaccggaat gttatcattg
    31741 agattcgtgg cggcgccggc ggcgatgagg catctctgtt cgctgcgtca ctttaccgga
    31801 tgtataccat gtatgcagag acccagggct ggaaacagga agttctcagc gccaatgaaa
    31861 cggaactggg cggctataag gaaatcagct tttccattct gggcgagggt gcttactccc
    31921 gcctgaaata tgagagcggc gtacaccggg tgcagcgtgt gccggaaacg gaatcccagg
    31981 gacgcatcca cacttctacg gttacggtag cggttctggt ggaggcggat gaagtggcgg
    32041 tggagatcaa cccaacagat ctgcggatcg acgtgttccg tgccagcggc gccggcggtc
    32101 agcatatcaa caagacggaa tctgctgtgc gcatcacaca tatccctacc ggtctggtgg
    32161 tggaatgcca ggacgagcgc agtcagtata agaataaaga caaggcgatg aaaattctcc
    32221 gttcccggct gtatgaacgg atgcagcagg aacagaacga taagatcgca tcagaacggc
    32281 ggatgcaggt gggaacgggt gaccgttccg agcgcatccg cacctataat tttccgcagg
    32341 gacgcatgac cgatcaccgg atcgggctga cgctgtaccg gctggaagat atcatgaacg
    32401 gaaatctgga cgaagtattt gatgcgcttg ccacagccga cgaactggct cggctggcag
    32461 gacagcagga agaggcgtaa aaacggtgga gacgaagtta ctttcagatc atgcggcgga
    32521 tttgctggag gcggcagcgc ttttgcagca gggaaaggtc gtgggaatcc cgacggagac
    32581 agtatacgga ctggcggcag atgccagaaa tccggaggct gtgcggcaga tctttgcggc
    32641 aaagggcaga ccggcagaca atccgctgat cgtgcatatt gcagagatgc aggcgttgga
    32701 gcaggtggca gcagttgtgc cgccgctggc ggtaaagctg gcagagcgtt tctggcccgg
    32761 tccgctgacc atgatcctgc ccaagcgcag cgatatcccg gagatcactt ccggcggtct
    32821 ggatacggtc gggatccgga tgccgtctca tcctgtggca agagcgctga tcctgctgtc
    32881 gggtgtgccg atcgcagcac cgtccgccaa catttccggc tatcccagtc ccaccacggc
    32941 acagcacgtg atgcgggata tgcacggcaa gatcgccgca gtggttgacg gcggcggatg
    33001 ttctgtgggc gtggagtcca ctgtgatcgc tctggaaaat gacgacacag tacggattct
    33061 gcgtccggga tatgtcacaa aggaaatgct ggaagagatc gtgccgcagg tgacgctgga
    33121 tccggcgatc ctgcatcagc tgaaggaagg tcagacggtg cgctctccgg gcatgaaata
    33181 tcagcactat tctccgcagg cacacgtgat cctggtggag ggagagtggc agaactttgc
    33241 ggcgtatgcc gaacagaaca aagcagacgg tgtgtatgca ctggtgtttg acggagagga
    33301 gacccgactc tccctgccgt gcctgaccta cggggcatcc ggtcaggaac aggcacagca
    33361 gattttttca aaattgcggg aattagatga cagaggtgcg caaaaggtat atgtgcgtgc
    33421 gccaaagccg gaggggatcg ggcttgcggt atacaatcgt ctgattcgtg cggcaggatt
    33481 tgaggtgata caggtatgaa cagtctcgat ggtgtgatgg tgatcggctt gaccggtcag
    33541 accggtgccg gaaaaagtac ggtttccaag gtctttgtac agaatggctt tcggctcatt
    33601 gatgcagatg ctatttctcg tcatgtggtt gcacgtggtt cccattgtct ggctgatctg
    33661 caggaatgct ttaccgatgc gatcttaacg ccggacgggg aactggaccg caaggtcatg
    33721 gcgtccattg ctttttcgga tcatcgcaaa ctcgaaatgc tcaataccat tatgtatccc
    33781 tatattgtgg gtgagatcct gcggatgata catcgctttt cccagcagaa tcacaagctg
    33841 atcttattgg atgcaccgac gctgtttgag agccgggcag acgatttttg tgacttgatt
    33901 atttctgtgg tggcaaagcc ggaactgagg atgcagcgga ttatggaacg ggatcatatt
    33961 tcagagaaag cggcgcagca gcgcatggaa atgcagctga cggaagagtt tttccggctg
    34021 cattcggata ctgtgctgga aaacaactcc agcttttctg agctgtggga tgcggcacag
    34081 gagctgtcgg caaagctgat ccgttattat gaaaccaagt tcggtgcatt tgtgtaaaga
    34141 cttgttttat ctgccttcct gcactgtgcg tcagggtacg gcatatcaaa caagatccaa
    34201 aaagtcctga gaattggagg ctatttctaa tggaatcgaa ggtaacacag gcaaaagaaa
    34261 aaaaggaaca gctgtttatg cagtccaagc acggcgcaaa ccgcatttct gcggagacac
    34321 agaagcagag cgatgctttt gcggcagact atatggcatt tttgaatgcg tccaaaaccg
    34381 agcgtgaggc tgcaaagaat gcggcggctc tgctggagga aaagggattc gttcccttta
    34441 ccccggatat gaaactccag gcaggggaca agatctatgt aaacaaccgt ggcaaggcga
    34501 tcattgccgc tgtgatcggt actgcaccta tcacagaggg cgtgcgtctg tgcgctgcgc
    34561 atattgactc gccccgtctg gacatgaagc agaatccctt gtatgaggat cacgagctgg
    34621 caatgttcaa gacccattat tacggcggca tcaagaagta tcagtggaca acgattccgc
    34681 tttctctgca tggcgttatt gtaaaagcag acggcacttc tgtagaagtc aacatcggtg
    34741 aagatgccgg cgatccggta ttttgtgtga ctgacctgct gccgcacctt gcaccgcagc
    34801 agatgaaacg cccggcaacg gaaattgtgc gtggtgagga actgaacctg ctggtcggct
    34861 caatgccgtt taacaaggat gaggagagcg agcaggtaaa gctgaacatt ctgaacatcc
    34921 tgcatgaaaa gtacggtatc actgagaccg atttcctgtc ggctgaactg gaggctgttc
    34981 cagcattccc ggcaagagaa ctgggctttg accggagtat gatcggtggt tatggacacg
    35041 atgaccgtgt ctgcgcttat ccggcactcc gtgcgctgct ggactgcgaa aagccggtac
    35101 acaccgctgt tacaatcctg accgataagg aagagaccgg cagtgacggc aacacaggtc
    35161 tctgctcttc ttatctgccg tacttcctgg aagatctggc agactgcttc ggggcaaagg
    35221 gtcgccacgt aatgcacgca tcacagtgcc tgtccgcaga tgttaacgca gcatttgatc
    35281 ccacattccc ggatgtgatg gagcagaaga actgcgcata tctgaatcac ggtgtatgcg
    35341 ttacaaagtt caccggcgct cgtggcaagt ccggcacatc ggatgcctcc gcagaatttg
    35401 tcggcacgat tcgccgtgtc ctggatgccg gtgatgtggt ttggcagacc ggcgaactgg
    35461 gcaaggtaga tgcaggcggc ggcggtacgg ttgcggctta tattgcaaac ctggatattg
    35521 acaccattga cgtgggcgtt ccggttttgt ccatgcacgc accgtttgag gtggtttcca
    35581 aactggatgt ctttatgaca tatcgtgcat tctgcacctt tattgcatcg taagcacttt
    35641 cttttgatat gaaaccgaat gccggcgagt tgaaattcgc cggcattttt gatgtgaggt
    35701 ggttgcgtgg agctttttca caagagaacc aagacgcagg cgattccgga cggattttcg
    35761 gcatcggaca ttcgggcaga gtccagcacc
//
LOCUS       DAWENO010000004        34032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_525_length_34032_cov_9.038026, whole genome shotgun sequence.
ACCESSION   DAWENO010000004 DAWENO010000000
VERSION     DAWENO010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 34032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 34032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34032
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_34032_cov_9.038026"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1602..1611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3047..3056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14881..14890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16310..16319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18640..18649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19076..19085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    31026..31035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    32530..32539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8982 a         8343 c         9113 g         7514 t
ORIGIN      
        1 gatctggacg gtacacttct gccgtccagc aaggaaattt ctgcggcgga cgctgcggca
       61 atttcacagt ttcgtgcaaa aggcggcaag tttgccattg cgaccggacg aacattacag
      121 gcagcacagc gctacttaaa taagctgaaa ccgaacattc ctgttatcct gttcaacggt
      181 gcggcaattt atgaccccgt cacagaaaag tggctctata cggaaaagct gccggcggac
      241 gctgtcgctg tcacacgcca ggtgctggat gcgttcccgg acgtctccgc cgagatcctg
      301 cggacagacg gcacttatgt ggcaaggatg acgccttatg aaaaagagca tctggaaatc
      361 tgccaggtcg aaccgattct ggcggaaccg gaagagatcc ccgaggggtg gctcaaggtg
      421 ctgtttgcga ttgcaccgga gcgaatgccg gatttaatcg catattttgc agcgcaaaac
      481 tgggactgtg cagactttgt acagtcggag gctcgctttt atgaaatgct gcccaagggt
      541 gcaaccaaag gcagcgcact gcggcgatac cggacgctgt gcggcgcaga gaactggaaa
      601 atcacagcag cgggggactt tgacaatgac ctggaaatgc tccgtgccgc tgacatcagc
      661 gcctgcccgt caaatgcaca gccttgtgta aagaaaatcg ttgatattca gttgacgcaa
      721 tcctgcgaaa ccaacgcaat tgcggaactg atccatcata ttacgaaatc attggaggtt
      781 catagtatgg atgaaatgac caagaaacag ctacaggcaa cagcttgtcg cattcgtatg
      841 ggtgttgtag aaggcaccta tcatgcaaag tccggtcatc cgggcggttc gctttcgatc
      901 tgcgacacgc tgacttacct gtattttgca aaaatgcacg tagatccgaa acagccggaa
      961 atggcagacc gtgaccgttt ggttttgtcc aagggtcact gcgcacctgc actgtattcc
     1021 acactggcag agcgtggctt tttcccgaag gaagagctga agtctctgcg tcatatcggc
     1081 gcaatgctgc aaggtcatcc ctgcatccat attccgggtg tagatatgtc cagcggttct
     1141 ctgggacagg gtatttccgc tgcctgtggt atggcactgg ctggcaagct ggacagtgca
     1201 tcctataagg tatacaccat cctgggtgac ggcgagatcg aagaaggaca ggtctgggag
     1261 gcagctatgt ttgcaaacca gtatcacctg gacaacctgg tagccatcgt tgacaacaac
     1321 ggtctccaga tcgacggcaa gatctccgaa gtctgctctc ccgagccgat tccggagaag
     1381 ttcgaggcat tcggctggca cgtgatccgg atggatgcac atgattttga ttccattgat
     1441 gcagcaatgc aagaggcaga atctattgtc ggcaagccgg tagtaatcat tcagaagagt
     1501 gtcaagggca agggcgtttc ctttatggaa aaccaggtcg gctggcacgg caaggcgccg
     1561 aatcaggcag aatacgaaca ggcaatggct gaactgacag cnnnnnnnnn nagacacttc
     1621 gtgatctcgc agaaaaatac ccggaactgg ttgttctgga tgctgacctg gctgccgcaa
     1681 ccaaaaccgg catcttcaag aaagcatatc cggagcgttt cttcgactgc ggcattgccg
     1741 aggcaaatat gatgggcgta gctgccggtc tgtccaccgc aggcaagatt ccgtttgcca
     1801 gcaccttcgc tatgtttgca gcaggcagag catttgaaat cgtccgcaac tccattggtt
     1861 acccgcatct gaatgtaaag atcggcgcaa cacacgctgg catttctgtc ggcgaagacg
     1921 gtgcaacaca ccagtgcaac gaggatatcg cactgatgcg cacgattccg ggcatgacca
     1981 ttttaaatcc ggcagatgac gttgaggcca gggctgctgt tgaggctgcg atccagtatg
     2041 agggtccggt atacctgcga ttcggcagac tggcaacccc gatcttcaac gatccggcaa
     2101 cctatcagtt taccatcgga aagggtatca ccctgcgcaa gggaacagat gtcacactgg
     2161 ttgcaaccgg tctgatggtc tgggagtcca tgaaggctgc tgaaatgctg gaggcagagg
     2221 gcatccacgc acgtgtactg aacattcaca caatcaagcc gctggatacg gaactgatct
     2281 gcaaggcagc atcggaaacc ggtctgctgg taacagttga agaacacagc atcatcggcg
     2341 gtctgggcgg cgcagttgca gaggctgtca cttcctgctg tccggttccg gtagttcgca
     2401 tcggcgtaaa tgacgaattc ggtcattccg gtccggctat tgatctgctg aaggaatttg
     2461 gtctgtgtgc agagaatatt gcagcagtta caaaaaaagc gctggcagaa aagaagtaat
     2521 tcatatcaaa ttttgaaaat agaaaaagca ggcgtttcct tttttcgagc gcctgctttt
     2581 ttacaaattg gagaaagaaa acggaggaaa gtgagatgag tgaaaaacaa ttaagagtgc
     2641 agacagtaga aaaactgttt ctctattttt tgatggcagc agtcatcggg tggtgctatg
     2701 aggtctttct ggaagtcgta gtctatcgct ggggatttga caatcgtggt gtgctgtttg
     2761 gcccctattg tccggtatac ggtgtcggaa tgctggcatt tctgctttgt ttcggaaaat
     2821 tcgcacaaat gccgatgaaa tggagcatcc gctgggcaag accaattctg atatttctgg
     2881 ggtgcggcgc tgtggcaact gttatcgaat tatgcgccag ctacctgctg gaatggatta
     2941 ccggcagctg gccgtggcag acatatgcag actatgacat caacttccag gcaagagttg
     3001 cgctttcgcc atccgtccgt tttggcattg gaggcgtgtt gtttctnnnn nnnnnnatct
     3061 gctccgtccg ctgtttgcaa aaataaccgg ctgtatgtcg ccaaaggtct tgaaatacac
     3121 cagcatcatt tgcgccggca ttctggcagt tgatttgatc tgtaccgttg ttttcaagct
     3181 gaccgcatga aagaaaaccg aaaaagctgc atattctttt ccagaggtga tatccaatgg
     3241 tgtgtacgct ggaagaactg cgcagcaaag atgtaatcaa tgttgtcaac ggtgaaaatc
     3301 tcggaagaat cgacgatttg cagatgaatg cacagacggc atctgtaacc gcactgatcc
     3361 tgtacggcag accacggctg tgggggattt ttggcccacg ggataattgt atcatagatt
     3421 ttcgccagat tcgtctggtc ggaaaagacg ttatcctggt ggaactgggc aagatatgta
     3481 cagactgcac aaatatcaag ttaaactaac acaaaatttc tgcatggaga aacttgaccg
     3541 gacaacacgt tttttggtat aataataagg cagttcacac gtctttcttg gtgtatgcgg
     3601 gtgctttgca ggaatgcaga gccggataca cccataagaa cggcggagga agcaaaacca
     3661 ttttatttag gaggactaca caatgggcgt agtatctatg aagcagctct tggaagctgg
     3721 cgtacacttt ggtcaccaga ccagaagatg gaacccgaaa atggctccgt acattttcac
     3781 cgaacgtaac ggcatctaca tcatcgacct gcagaagacc gtaaagaaac tggatgaggc
     3841 ttatatgttc atccgtgacc tgtctgcaca gggcgagtct gtactgttcg ttggtacaaa
     3901 gaagcaggct ggcgattcca tcaaggaaga ggctacccgt gctggtgcgt actatgtaaa
     3961 cgcacgttgg ctgggcggta tgatgacaaa ctttgcaacc atccagagaa gaatcaagcg
     4021 tctggaacag ctgcacgaga tggaaagcga cggcacattt gacctgctgc cgaagaagga
     4081 agttggtaag ctacagctgg agattgaaaa gctggagaag ttcctgggcg gtatcaagga
     4141 tatgaagaag ctgccgggtg cactgttcat cgttgacccc cgcaaggagc gcatcgctgt
     4201 tgcagaggca aagaagttga acatcccgat cgttgcaatc gtagacacca actgcgatcc
     4261 ggatgagatc gactacgtta tcccgggtaa cgatgacgca atccgtgctg taaagctgat
     4321 cgctggcgtt atggctgatg caatcatcga aggccgccag ggcgctgacg ctgctgctgc
     4381 agaggctgct gaaaccgcag aggctgaggc tgctgaataa gcaaaactga aatatctgtg
     4441 aaatcagaaa cccgaatatg tctggcgaat ccgcccctat tcgggttttt tgagagaaac
     4501 atttgaaaat aggattagga ggaaacatct aatggctaat tttactgcaa aagatgtcaa
     4561 tgaactgcgt aagtccactg gcgttggtat gatggactgc aagaaggctc tgactgaggc
     4621 tgacggcgat gtagaaaagg caattgaact gctgcgtgaa aagggactgg ctactcaggc
     4681 aaagaaggct ggtcgtgttg ctgcagaggg tatggttgtt gctaaggtta acgctgacaa
     4741 cagcgtaggc tgcgttgttg aagtaaactc tgagactgac ttcgttgcaa acaccgatga
     4801 attcaaggca tttgtaaacc aggttgctga taccattatc gaaaagaatc cggcagacgt
     4861 agagactctg aaggctacta ctgtatccgg cggcacaatg actgttgacg aagcactcca
     4921 ggaactgttc ctgaagattc gtgagaacct gcaaatccgt cgtttcgttc gcatggaagg
     4981 catcctcgta ccgtatatcc acggcggcgg caagatcggc gttatggttc gtgcagaatc
     5041 tccggctggc gcaacaccgg aagttctggc tgctgcaaag gactgcgcac tgcaggttgc
     5101 tgcaatgaac ccgccgtatc tgaagcgtga agacgttccg gcagctgttc tggacgaaga
     5161 aaagaaaatc atcctggctc agatggcaga ggacccgaag atggcgaaca agccggaaca
     5221 ggttaaggaa aagattgccg ctggtaaggt tggcaagtac tatggcgaaa actgcctgct
     5281 ggaacaggct ttcgtcaagg aaaacaaaga aaccgttcag agctatgtag acggcgttgc
     5341 aaaggcagct ggcactacaa tcgcaatcac tgattttgtt cgctttgagc gtggcgaagg
     5401 tatcgaaaag aaggctgcca atttcgcaga agaaattgca agcatgatga agtaagatac
     5461 ccctaaaatt tatgaattgt gcattgccat tttgagggca atcgtgctga aagagggagc
     5521 atggcggttg tcatgctctt tttttatttt tttccgaaaa tgactttaca tttccggaga
     5581 aatagtgtaa aatagacata tggactgtct ttccaaaagg aaggcatcat ttgtcagact
     5641 gcggtacggg agtctgaata ccgagtaaca gacgaaagga atcaagaagc atgaaaccga
     5701 aatatcatcg agttcttctg aaattaagcg gcgaggcact tgccggtccg aagaaaaccg
     5761 gactggacgc agaaacaatg ctgccgattt gcaaaagcat taaaaagtgc gtcgatgcag
     5821 gcgtagaaat gggcatcgtt gtcggcggcg gcaacttctg gcgtggacgt tccagcgaga
     5881 acatggaccg caccagagca gaccacatcg gaatgctggc aaccacaatg aatgcactgg
     5941 ctgttgcaga tatgctggaa tctcttggac tggaagtgcg ggtacaaacc gcaatcagta
     6001 tgcagcagat cgcagagccg tatatccgca accgtgccat cacacatctg aaaaaaggca
     6061 gagtcgttat tttcggatgc ggcacaggca gcccgttctt ctcgaccgat accgcagcaa
     6121 ctctgcgtgc tgtagagatc gaggcagaca ttctgctgaa ggcaaccatg gtagacggtg
     6181 tttacgacaa ggatccgcac aagtatgaag atgcagttcg ttattcccgt ctgacattca
     6241 gcgatgttct gggcagcgca ttgcaggtta tggacagcac agccgcatcc atgtgccgtg
     6301 acaacaagat gccgatgctc gtgttcgatc tgagccgtcc ggaaaacatc tacgatgcag
     6361 tcatgggtga agatgtgggt acagttgtat ctgaggtcga ccccattgca gcaaaggcat
     6421 aaaccatcct attgcagcgt acactaaaaa tcgaaaggag tcccgtatat cgggaaaata
     6481 ttatgaaagc gattattaaa gaaacagaag ataaaatgaa gaagtccacc gaccgcctgg
     6541 cacacgagtt taccacaatt cgtgctggcc gtgcaaatcc ggctgttctg gataaggtac
     6601 aggttgacta ttacggcgtg ccgaccccga tccagcagat ggctgctgta tccgtttctg
     6661 aggctcgcat cctggtgatc cagccgtggg atgtcacttc tctgaaagcc attgagaaag
     6721 cgatcctgtc ctctgatgta ggcatcacac cgaccaacga cggcaaggtg ctgcgtctgg
     6781 tattcccgca gctgaccgaa gatcgccgta aggaactctg caaggagatc aagaaactgg
     6841 gcgaagagtg caaggttgca gtccgcaatg ttcgccgtga ttccattgaa aagctgaaag
     6901 caaagaaaaa ggcatccgag ctgaccgagg atgaagtaaa ggacggcgaa aagcagattc
     6961 aggacgtaac cgataagtac gttgctgaga tcgacaagat ggtatctgaa aaggataagg
     7021 agatcatgag catctgacga tgcacggtcc catccagtcc tggaaacaga ataggagatg
     7081 aactgtatgg ctctgccatt tttcaacaaa aaaacttccc ccaaggacga accaaccacc
     7141 ggcgctattg cactgccgga acagctgccg cagcacgtag gctttattat ggacggcaac
     7201 ggcagatggg caaccaagcg tggtatgcca cgaaaattcg gacaccgtga aggtgcgaag
     7261 aatttcaaag acctgtctct gtactgccgg gacattggtc tgcgaaatgc ctctttttat
     7321 gttttctcca cggagaactg gaaacgaccg caggacgaag tggacgccat catggatttg
     7381 ttctgggatt acctctgtga ggcagatcag tacctggaac gccgtgtgcg catggtgttt
     7441 gtcggcgacc ggagcagact ccaggatcgc ctgcaaaagc gcatggaaga actggaacac
     7501 gattccaggg actttgaaaa gatgaacctg attctcgcca tcaactacgg cggcagagat
     7561 gagatcaagc acgcaacgca ggaaattgcc aagcaggtac aggcaggaac actttctccg
     7621 gacgatatca ccgaacagac gattgcagac catctctata cagccggtct gccggatgtt
     7681 gacctgctga tccgtcccag cggcgaactg cggctgtcca actatctgat ctggcagtgc
     7741 gcctatgcag agtattactt caccaatatt ctgtggccgg acttcaaccg aaatgagctg
     7801 aaccgggcat tggcagattt cgcacagcgt ggcagacgat ttggaggcat ctgagtatga
     7861 aagtacgtat catctccggc gtcgtcggcg caattctggc agttgcaatt ctgctgctgc
     7921 ataaaacatt tgtttttccg ctggcaattg cagcgctgat ctgcattgct ttgtttgaac
     7981 tatttcgtgc agggaagtgc ctgcactgcc gcatttccat cggcgcaggt ttcctgtacg
     8041 gtgcggcact gccgtttttg cagtactacg gaaactatac cgcagtgatt gcactgacat
     8101 tcctgtgcat cattgccatg tttgcggaaa tggttctgct gcataagtgc gtgacatatc
     8161 agtctacatt gtttattgca gcaatcaccc tgctggtatc gcattccttg agtatgctaa
     8221 acacactgct gcacatcagc agctatggtc tggcaagtgt gatcctggcg ctctgttctg
     8281 cttgggtggc agacaccggc gcatacttcg ccggaacatt cctgggcaaa cacaagctct
     8341 gcccggaaat cagcccgaaa aaaaccgtgg aaggtttctt cggcggcatt gcggcagacg
     8401 taatcttcat ggtgattttc gcactgatct acagcttgat tgcacacgct catgtacatt
     8461 acctgtggct tattttcatt ggtattgtgt gtgcagttat cagcgtgctg ggcgatctga
     8521 gcgcatctgt tctgaaacgc cagcagggaa tcaaggattt cggaaacatt atgcccggac
     8581 acggcggcgt aatggatcgc tttgacagcg ttctgttcac tgtcccggcg ttctatgcgc
     8641 tggaaatgat gtacccggta ttctttggat aattgtatag gctctcgggc aatactgccc
     8701 acacaccaca cacgatgcag ggcgctttgc tttgtgtcgt gtgtggtgtt tatgagcctg
     8761 tttcaaaaag gagacagaaa aaatggggaa atgtatttca atactaggct cgaccggttc
     8821 gattggcacg cagtcgctgg aagttgcaaa ggcacatgat atgcaggtcc atgccctggc
     8881 agcaaatcga aatattacac tgctggaaca acagacacgg gaatatcacc cgaaaaaggt
     8941 ctgcattttt gaagagagcc agtatcagga actgaagcag cgtctggcag atttgccggt
     9001 acaggtgctg tgcggcatgg atggactctg cgacatcgca gcagataaaa acgcagatat
     9061 cctaatcaat tccgttgtag gcatggtggg acttcttccc acactgaccg ctatttctgc
     9121 cggcatggac gttgccctgg caaacaagga aacactggtt gccggaggtt cactggtcat
     9181 ggaacaagct gcccggaagg gtgtgcatat ctatcccgtg gacagcgaac actccgccat
     9241 cttccagtgc ctgcaaggaa atcgccggga acagctgaac aagatcattc tgactgcatc
     9301 cggcgggccg ttcttcggaa agactcgtgc ggaactggaa aatgtcactg ttgcagatgc
     9361 actgaatcac ccgaactgga gcatgggcaa caagatcacc attgactctg caacgctgat
     9421 gaataaggga ttggaattca ttgaggcaaa gtggctcttc gatctgaccc cggatcagat
     9481 cgagatcgtg gtacatcgtc agagcgttgt gcattcggcg gtagaatacc aggattgttc
     9541 cgtcatcgct cagctgggcg taccggatat gaaaattccg attcagtatg ccctgctgta
     9601 tccggagaga atgccctgcc cgacaaagca tctgtctctg acagattacg gcacactgac
     9661 cttcgaaaaa ccggatatgg agacctttac ctgtctgaaa accgcaattg cggcaatcac
     9721 cgccggcggt acagcaccct gcatcgtaaa cggggcaaat gaggttgctg ttgcagcgtt
     9781 cctggcggga aagatcggtt tcctggaaat cggcgcactg gcacaacagg cgatggcatc
     9841 cattccgcct acagcaatca aaacatatga ggatgttatg aaagcagata cacaggcacg
     9901 ggagttcgtt cgaaacgcca ttgcatagaa aggaaacagc atatgcagca cattgtaact
     9961 atcctcatcg caattctgat tttttttatc attgttgtca tccatgaatt cgggcatttt
    10021 atcgctgcga aactgaaccg cattcaggtg aatcaatttg ccgtcggtat gggcccgaaa
    10081 cttctgcact tccaaaaagg ggagacagaa tactctctcc gcctttttcc tgttggcgga
    10141 ttttgtgcca tggagggcga agatcagtcc agcgacaatc cgaacgcatt tgaaaaaaag
    10201 ccggtgtggc ggcgcatgac cgttatactt gccggtccgt tcatgaattt ggtgcttgga
    10261 ttcctgctga tagtgatact gctttgtact gcacaaaaag tcccttctct gaccattgcc
    10321 cggtttgcgg agaccaccaa tgcagccggt gaaaccgtaa ccgctgcgga aagcgaacgc
    10381 tctggtctgc aagtcgggga taagattgtt gccattgacg gcagccacat tttttcaacc
    10441 agcgacttga tctataagct gcaaaccaca gacaccgaaa cctacgacat taccgtaaaa
    10501 cgagacggca agcgtgtcac cattgaaaac gttacgttcc acaatgacaa cacagacggg
    10561 ctgctggatt tcgcagtgga aggaaagccg aaaaatccag tcacgatcgt ttcctatgcg
    10621 gcaaaagata cggttgcaac cgcaaagctg atctggatgt ctctggtgga actggtaagc
    10681 ggtaagtatg gtctgcagga tctttccggt ccggtcggca ccgtcagcgt aattgaacag
    10741 gcagcctcca gcggagataa tttcctggaa cggtttcagt ctgtcataaa tctgacgatc
    10801 ttcattaccg taaatgtagg cgtgttcaat ctgctcccca tccccggtct ggacggcagc
    10861 cgttttgtgt tcctgctgat cgaggcaatt cgccgcaagc ccattgccaa agaacgggag
    10921 gcactggtgc atttcatcgg catggcagct ttgttcctgc tgatgatcct ggtaaccata
    10981 caagatgtgg gacgcttttt tcaaaaatag ggagtgacat atatgagaaa tttgaaacct
    11041 gtaaaggtcg gaaactgcac gctggacggc agtcatatct atatccagtc catgctgaac
    11101 actcgttccg atgacattgc cggcagcgtg gcacaggcag tggcactgga acaggcagga
    11161 tgtgagatcg tccgtgctgc aatcccgaac cacgaggcaa tcgcactaat tccggcaatc
    11221 aaagaaaaga tctcgattcc gctggttgcc gacatccatt ttgactaccg cctggcgctg
    11281 gaggcagcag aggcagggat tgataagatc cggatcaacc ccggcaacat cggcggcatg
    11341 agccgggtgc aggcggtcgt aaaagcctgc caggcaagaa atatccccat ccgcatcggc
    11401 gtcaactccg gctcgctgga aaaagaaatc ctggcaaaat acggtgcgcc tacagctgag
    11461 gcattggttg aaagcgccat gggacacgtg cagatgctgg aggcgtgcgg ctttgatgat
    11521 atcgtcatct ctatgaaatc atccgatgtc aacaccatga tcgacgctta ccgcctgtgt
    11581 gcgcagcagt gtagctatcc gctccacctg ggcgttacag aggcaggcac agagcgaatg
    11641 ggtctgatta aatcggcaat cggcatcggt tcactgctcc acgacggcat cggcgaaacc
    11701 attcgtgttt ctctgacaga agacccggta aaggaaatca aggctgccag agatattctc
    11761 aagtgtatcg gcaagcgtgg gggaccgcag atcgtctcct gtcccacctg cggcagaacc
    11821 cggatcgacc tggtgaaaac tgccaaggaa gtagaggctg ccctagaaaa cgtggataag
    11881 gacatcaagg ttgcagtcat gggctgcgtg gtaaacggac ccggcgaggc aagagaggca
    11941 gacatcggaa tcgccggcgg cgacggatgt gcaatgctgt tccggaaggg gactgttctt
    12001 cgaaaggttg cggaggagga catcgttccg gaactgatgg cagagattga aaaactctaa
    12061 ggtacaataa taggagtgga acatatggat ttgaatttcg cagagttttt ggaagaatac
    12121 gtccccataa tgccggagtc cgtacaggac ggcgtgctga aacggatgac atattcggaa
    12181 aacctgacga aaatttcgtt ttacattgca ttttcgcatc tcgtcccggc acaggatctg
    12241 attgcactgg aacatgaact ggaaatccgc ctggaaatcc acgccgtccg gctgcatcca
    12301 cgctatacac cagaattgtt cacgatgaca tatttcccgg atctggtcaa gtcacgcaaa
    12361 cggcaaccga ctgtactcaa cggattcatt gacggcgcag aagtgacgct ggacggtgac
    12421 aacctgcata tccagctgca caacggcggc tatgatatct tgcaaaaata tcatgtggct
    12481 gactttttct cgggcttgct ggaacagctt ttctcaaagc cgtttcatgt ggaattcctg
    12541 ggagacgcca gcgtcagcga tgaccagttc caggcaatga tcgcacaggc agaaaaggaa
    12601 ctgcccaagc acagcgaaca gttccaggcg cagccgtctg cacctgcacc gtctgccggc
    12661 ggcgaaaaag ccccgaagcc taccgcacag agcgttgcca tgaacgaaga tattcccgga
    12721 gttgtaccgg gcagcgctgt cttgatcaaa ggcagaccta tccgggatat tcccatgccg
    12781 atacgggagg ctgtggaggt acagaaccag cgtgtggttg tcatgggcga tgtattcgcc
    12841 atggattccc gtgaagttcg tggggaacga accattctga cctatcagat cacagacggg
    12901 acttattccg tgctggtcaa gattttcgac acaaccgaaa aactctcaaa aatgccgttc
    12961 ggtgagatca aaaaggacag tacactgctg attatcggac gtatcggcta tgacgatttt
    13021 gcccgggaag acgttctgaa accagaatcc atcgtcatgg taaagcgtca gcgaaaaatg
    13081 gacaatgccc ccaaaaaacg tgtggaactg cactgccata caaatatgtc tcagatggat
    13141 gcagtcacac cctgtgagcg gctggtacag cgggcgcatg agtggggaca tccggcaatc
    13201 gccattacag accacggaat tgcacaggct tttccggacg caatgaacgc ctggcgtggt
    13261 ttcaaggaca aagatttcaa ggtgatcttc ggctgcgagg catatgttgt caatgacttg
    13321 aaccgtctga tggtaattga caatcccgga gaccgcacct tacaggatga gatcattata
    13381 tttgacgtgg aaacgacagg actcagcccg actcatgacc ggctgaccga aatcggcgct
    13441 gtccggatgc gtggaatgca ggtggtggaa accttcaaca ccatggtaga cccggagcgg
    13501 ccgatcccgg caaagatcgt cgaactcacc ggcattacag atgctatggt ggagggtgcg
    13561 ccaaaagagg cagaggcact gcgaaagttc atggaattct gcggcgaaaa tccggttctg
    13621 gctgcccata atgcaaagtt tgacacctca tttgtccgca atgtctgcaa gcggcagcat
    13681 atcacctttg acttggcaac actggacacg ttgggtctct gcaaatgtat gcttccgacc
    13741 atgtcccgtc actgactgga cggcgtagca aaggcgctga aactgggaaa gttcgaccac
    13801 catcgtgcct ccgatgacgc acagatgctg gcaaagatct ataaagaact ggtcagccgt
    13861 ctgatacaga caaagggcgc tgttctgctc tctgatctga acaacaagac ccaggagata
    13921 gacgttaaaa agctgaaatc attccaccag atcattctgg tacgcaacca cattggtctg
    13981 aaaaatctat acaagctgat ctcctatgga cacatagagt gtttctatcg aaagccgctg
    14041 attccgaaat cagttctgat gcagcaccgt gaaggcttga ttttcggcag cgcctgcgag
    14101 gctggtgaac tcttccaggc gtttgtagac gagagacctc atgaagaaat tgtccagctg
    14161 gcaaagtttt atgattacct ggaaatccag ccggcaggga acaacgcctt tatgattcga
    14221 aacggcacag ctgccaacat cgaagagttg caggaataca accgaaagat cgtagcgctg
    14281 ggcgacgaac tgggcattcc ggtctgcgcc acctgcgacg tgcatttcat ggacaaggaa
    14341 gatgccattt accgtgagat cttgcaagcc ggacaaggct atgaagatgc tcgctttcag
    14401 ccgccgcttt atctgcggac cacagaggag atgctggacg agttctccta cttgggcgaa
    14461 gaaaaggcat acgaaattgt cgtgacgaat accaacgccg tggcggacca gatcgagcgc
    14521 atccagccgt tcccgaacgg aacattcacc ccgaccattg acggcgcaga agaggacctg
    14581 gtgcgcatca ctaacaccaa ggcaaaggag atctacggcg atccgctgcc ggaactggta
    14641 gaaaagcggc tgaaccggga gctttcttca attatcaagc atggtttctc tgtgctgtac
    14701 atcatcgcac aaaagcttgt ctgggacagt gttgcacatg gctaccaggt aggttcccgt
    14761 ggttctgtcg gttcttcttt cgtggcatcc atggcaggaa tctccgaggt aaatccgctt
    14821 gcgccacact acgtctgccc gaaatgcaaa cacagtgagt ttatcacaga cggcagcatt
    14881 nnnnnnnnnn cgaaaaactg cccggaatgc ggtacagaaa tgaaccgtga cggacacgac
    14941 attccttttg aaacattcct gggatttgac ggcgacaaag cgccggatat cgacctgaac
    15001 ttctccagcg aataccagtc ctctgcacac cgttacaccg aggagctctt cggacgggac
    15061 aacgtgttta aggcaggaac gatctcggcg ctggcagaaa aaacggctta tggctacgtc
    15121 aaaaaatatc tggaagaaaa gggactgcac tacagcccta cagagatcaa ccggctgtcc
    15181 atcggctgta ccggcatcaa gcgaactacc ggtcagcatc cgggcggcat ggtcgttgta
    15241 cccagtgact atgacgtata cgacttcaca ccggtacagc acccggcaga cgatgcggag
    15301 tccgaaattc tgaccacgca ttttgacttt cactccctcc atgataccat tctgaaactg
    15361 gatgagctgg gtcacgtcgt gccgaccatg tataagcacc tggaagatat gacaggactt
    15421 tctattcacg aagtccccac aacagacccg aaggtcatcc agatgtgtac agatgcctct
    15481 gttctcggcg taactccaga cgagatctac tgccagacag gcagtctggg catcccggaa
    15541 atgggtaccg gattcaccat tcagatgctg ctggactcca aaccgaaaaa gttcagcgac
    15601 ttcttacagg tcagcggact ttcgcacggt acagacgtat ggctgggcaa tgcgaaagat
    15661 ctgatcgaca acggcacctg caccatttca gacgtaatcg gaacacgtga cagtatcatg
    15721 acctacctgc tgcataaggg cgtagagcca aagcaggcat tccagatcat ggaggacacc
    15781 cgaaagggaa aagccccgaa aacctttacg ccggaacgtg tgcagatgct gaaagaccac
    15841 aatgtgccgc agtggtatat tgacagctgt ctgaaaatca agtatatgtt cccgaaagca
    15901 cacgccgccg cctatgttac cgccgccatc aaattgggat ggttcaagct gtaccatccg
    15961 ctggcatatt atgcggttta cttcagtacc cgtggtgacg actttgacgc agttcttgcg
    16021 ctccagggaa aagaggcagt ccgtgcaaaa atcgaggaac tgaaagaaaa gggcaacgaa
    16081 cgctccaaaa aggaatccga cctgttggat acgctgctta tcgtcaatga gatgctcagc
    16141 cgtggctgtg aattcctgcc gatcgacctg ttccactccc atgcgacaaa atatctggta
    16201 gaggacaaca agatccgcat tccgttcagc gccgtacccg gcatcggtat ttctgcggca
    16261 cagaacctgt acgatacagc gcaaaagcga aatttcattt ccatcgaagn nnnnnnnnna
    16321 tcccagagtg gcgcatcaaa aaccataatt gaaacgctgg aacagttgaa tgctatgggc
    16381 gatttgccga aatccagtca gatgacactc ttttaatgaa ttgtccttga cattggatgt
    16441 ttgatgtgct actatagtat tgtagtagca cgataaagcg ttaggaggaa cgtatgagaa
    16501 agtatgattt gattaccccg gaggggacaa aggacttttt gtttgaagaa tgcctggtgc
    16561 gccgggaaat cgagcaggat ctgcatgacc tgtttcggag caagggatat tctgaactga
    16621 ctacaccggg actggaattt tttgacgtat ttcagtcaga atccggacat tatccgcagg
    16681 aacgcctgta taagctgaca gacagcaagg gacgtctgct ggtgctgcgt ccggaatcca
    16741 ccatgcccat tgccagagtt gcggcgaccc gtctgcggga tgcgtccctg ccgctgcgtc
    16801 tctgctataa ccagaccgtg tatcgctttg aacctgcact caagggcaga agtgacgaga
    16861 tcacgcaggc aggcattgaa ctgctcggct cttcttctta cctggcggat gtggaaatga
    16921 ttacaacagc gatcgaatcc ctggcgtcgt tcagcagcga taatatcagc ttttccctgg
    16981 aactgggaga tgccggcgta ttcaaggaac tgatgcggga actgaatgcc acaccggaag
    17041 aacgtgaaaa cattcgctat ctgatcgaaa caaagaacta tccggcactg aatgacgttc
    17101 tggataccat ggatgatgta aaagttgccg ctgcgctgaa aaagctgccg gcactgtttg
    17161 gcggtgaaga agtctttgac aaggcatccc agctgtattc caacgagcgg atcgacgaga
    17221 ttctgatgga tctgcggaaa ctgtataagg acatttccac cgtgatcggc aagggcagac
    17281 tgaccgttga tctgggcatg gtcaacaatg cggactatta taccggcgtc atcatcaagg
    17341 gctatctgga aggctatggc gaagaagtgc tttccggcgg acgctataac aagctgttgt
    17401 ccgactttgg ctatgatatc ccggcaaccg gctttgcaat caatgtagat gccgttgcaa
    17461 atgtacgcat gaaagaccag ccggcacagc tgcagccggc agatgctgtg atttttgcag
    17521 cgcccggcta tgaaatgaaa gccgctaagg tatcccagga actgcgcagc aagggacagg
    17581 ttgtggagtt tgcatttttc gacagcctgg atctggtgcg tgagtatgca cgggaaagca
    17641 gaatcggaaa agtcgttgta gtagatgaag agattacgga ggtacagtaa atggcaaagc
    17701 cgctgagaat tgcattaaca aagggcagac tggaaaaaga cacagtcggg ctgttggaac
    17761 ggctgggcta tgactgcact gcggtgcggg aaaagggccg ccggctgatc ttgccgatcc
    17821 cggacggcaa tatggaagtc gtcctggcaa aggcaaacga tgtaatcacc tatgtagaac
    17881 acggtgtctg cgatatgggt gttgtcggaa aagatacgat cattgaaatg cagggcaagt
    17941 tcttcgaact ggttgacctg ggattcggca gatgcaagtt cgccctggcg tgcaaaaagg
    18001 gaaccgactt ctacagcggt ttcggtgtca agtgcattgc aacaaagtat ccgaatgtag
    18061 cacggcgctt ttttgaaagc aagggcatgg acgtagacat cgtcaagatc gaaggctctg
    18121 tggaacttgc gccgctgctg ggactggcag acggcatcgt cgatatcgta gaaaccggcg
    18181 taacgctccg tgaaaacggt ctggaagtcg tcgaggacgt tgtcccgatc tctgctcgtc
    18241 tgattgccaa taccgttagt ctgaaactgc ggcaaactga aattgaagct ttgattcaga
    18301 aaattgagga ggctttataa tatgattacc atgaaaacca ttaacgccag tgatctggca
    18361 cagcgagacg ctttttttga aagcctggac aagcgtaccg gcgaaaccaa ccagcgagtt
    18421 accgaaacgg taagcgagat catccgcacc gtaaaaaccg gcggcgatga ggcggttaaa
    18481 aactatacgc tgaaatttga cggcagcctg cctcagtact acgaagtacc gagagatgtc
    18541 atcaacgatg cgctgaccaa tgcagatccg gcatttgtag atgctatgct gaacgccatc
    18601 gaaaacgtcc gtgatttcca ccagcgccag gtgcaggagn nnnnnnnnng ctttatccac
    18661 gcactgccaa acggtgtaat tctgggacag cgtgttcgtg gtctggagcg tgttggtctg
    18721 tatgtaccgg gcggcacggc agcgtatccg tccagcgtca tcatgaatgc tgtaccggca
    18781 aaaattgccg gcgtgaagga aatcatcatg gtaacaccgc cctgcaagga cggtacaccg
    18841 aacccggata tcctggttgc agccggtctc tgcggcgtag accgtgtatt cctctccggc
    18901 ggcgcacagg cgatcgcagc cctggcatac ggcacagagg agatcccgaa ggtagacaag
    18961 atcgtcggcc ccggcaatat ctttgtggct accgcaaaga aactgctgta cggcgttgtg
    19021 gatattgaca tgatcgcagg tcccagcgaa gttctggtta tggcagatga aacagnnnnn
    19081 nnnnntcctg gcatctgcga ttctggtcac aacctgcccg gaactggcag aaaaggtaca
    19141 ggcagagatc aaccgccagg catcctacct ttccagaaaa gagatcatcg aaaagtccat
    19201 gaacgaatac ggcgcattcc tgctgtgtga cacccgtgaa gaggctgttg tcctggcgaa
    19261 tatggttgca ccggaacacc tggaagtcct catggagaac cccatggaac tgctgggcgc
    19321 actggacaat gccggttcgg tattcctggg cgcatatgcg tcagaaccgc tgggtgacta
    19381 ctatgccggt ccgaaccacg ttctgcccac cagtggaaca gcccgtttct tctctccgct
    19441 gggcgtgcag agctttacca agcgttccag ctacacctac tataccaagg acgctctgcg
    19501 tgcagccaag gacgatattg tgctgattgc caataaagaa gggctgacgg cacacgctaa
    19561 tgcaattacc gttcggtttg aggatgaaaa gcagtaacgg gaattcagac aacggaatcg
    19621 acagacaaaa cagcagctta tccgtgcggc aggaattttt caattgaatt tatctaacac
    19681 aaggaggaat ccatatgcgg caagcacaac ttgcacgaaa gaccaaagag acagacatac
    19741 aggtgaccgt aaaactggac aacatgggaa ccgcaaacat caacaccggc atcggctttt
    19801 ttgaccatat gctgaccgca ctgtctgttc acagcggcat cagcatggac attgccgtaa
    19861 agggtgacct ctatgtggac ggtcaccata ccgtcgagga caccggcatc gtcctgggaa
    19921 aggcgctggg tgaggcgctg ggcgacaagt ccggcatcac acgctacggc agtgcattca
    19981 ttcccatgga cgaggcgctg gcattttgca gcctggacat cagcaaccgc ccgttcctgg
    20041 tattccaggg gacattcacc aacgctatga tcggacagta tgacgcctgc ctgacggaag
    20101 aatttttccg tgcctttgca ttcaacgcag gaatcacact gcacgtcaat atgatgtacg
    20161 gttccaacga tcaccataag tgtgaggcgg ctttcaaggc agtcgcacac gcactgaaaa
    20221 ccgccgtaac gccgctggaa gacggcaaga ctttgtccac gaaaggagtc ctctgagatg
    20281 atcgcaatta tcgactatgg cgcaggcaac attttcagcg tcaagaatgc gctggactat
    20341 ctgggacttc ccggagaact gaccgctgat ccggaaaaaa tccgcaaagc agatgcgctg
    20401 atcctacccg gcgtaggggc atttcccagt gccatgcaga tgctgcacga cagcggactg
    20461 accgacgtga tccgggaaga atcccataaa aagccgttcc tcggcatctg tctgggaatg
    20521 cagctcatct ttgaaaaggg ttacgagttt gaagaatgcg acggtctcgg acttgtcccc
    20581 ggcaaggtag aaaaaatcga agcaccgggc atggtgattc cgcacatggg ctggaacaag
    20641 ctggaaatgc agaacgactg cccgattctg cgcaacctgc cggaagagtc ttacgtgtat
    20701 ttcgtgcatt cctaccaggc agttgtgccg gacgaatacc tggcagccta tgtagaatac
    20761 ggcacggcaa aaatcccggc aatggtatat gacggcaaaa ccgtctacgg cgcacagttc
    20821 cacccggaaa agagcggcga agtcgggctg caaatcctgc gaaactttgg agggctgatc
    20881 ggatgattat tttacctgca attgatatta aagacggtaa ctgcgtccgc ctgttcaaag
    20941 gcgatttctc taccgtggaa aaagtcgcag cagattacat ggaaaccgcc aaaggttttg
    21001 aaattgccgg cgcatcctgg attcacatgg tagatctgga cggcgctaaa gagggcagac
    21061 caatgaatca gcatatctac gaagatgtgg cagcaaaaac cggactgaaa gtggaagtcg
    21121 gcggcggcat ccgaaatctg gaaaccatcg acacctatct gaaaagcggc gtggagcgtg
    21181 tcattctggg ttctgcggcg ctgaaaaatc cgcagctggt gaaagatgcc gtgcagaagt
    21241 tcggcagtca gcacattgca gtgggcattg atgccaagaa cggcatggtt gccacagagg
    21301 gctggctgga agaatccgac accgattaca ttacactggc aaaagaaatg atcgcagtgg
    21361 gtgtgcagta ttttatcgta accgacattg caaaagacgg cacgctttcc ggcgttaatc
    21421 tggaacagct gaaagacctg cgagatgcca cagagggcaa atgcaatatc atcgccagcg
    21481 gcggtgtgca taccattgca gatatccgtg gctgtaagga actggggctg tacggcacga
    21541 tctgcggcaa gtcgatctat aagggtacat tggatctgcg ggaagccatt gcccttgcgg
    21601 aaggagtgta acatatgctg gcaaaacgaa tcatcccttg cctggatgtg cgaaatggca
    21661 aggttgtcaa aggtgtaaac ttcgagggaa tcaaggatgt aggcgacccg gtagaatgtg
    21721 cctatgccta taaccagcag ggtgcagacg agatcgtgtt ctatgatatc actgcatcct
    21781 atgagggcag aggtgtcatg ctggatgtgg ttcgggagac cgcaaaaaag gtattcgttc
    21841 cgctgaccgt tggcggcggc atcaaaacag tagacgatat ccgtgacacg ctccgtgccg
    21901 gtgcagataa ggtgtctgtc aattcccagg cagtgcagaa tccgcagctc atcaaagatg
    21961 gtgcagacat tttcggctgc cagtgcatct gcgtgggcgt agatgccaaa aaagtcggcg
    22021 agaacaagtg gaccgtattt atcaacggcg gccgtgtgaa tatgcagctg gacctggtcg
    22081 attgggtcaa acagtgcgaa cagctgggtg caggggagat ctgtctgaac tccattgata
    22141 ccgacggcgt acgtggtggt tttgacctgc ctatgctgaa agccgtctgc gatgcggtac
    22201 agattccggt tatcgccagc ggcggcgcag gaaagctgtc tgacttcgca gatgcgttcc
    22261 tggaaaccgg atgctctgcg gcactggcag cctctctgtt ccacttccgg gaactgacag
    22321 tccaggatgt caaggcagac tgccgtgcaa aaggcattcc catgcggtaa aataaattcc
    22381 aagacggata gcaaagggga aatggtttca tggtatttct gatgatctgc acatttctga
    22441 ttccggtaat ccaagtgatt gcgggatggg cgatgcgata tcacacgccg aaacaaatca
    22501 acagctggat cggctatcgc accatgcgtt cgacaaaaga cgaaactgcc tggttatttg
    22561 ccaatcgtta ttgcggaaag ctgtggctga ctgctggcgt tggaatgctg gtgcttacag
    22621 aatgcagcct gtttctgtcc cggaaagctg ccgatattgt ctcgcttgtg atgatcggcg
    22681 tgcagacgct tgaaatcctg ctgaccattc cttttgtaga aaacgctctg aaacaaaacg
    22741 aaagaggaga cactcatgct caaaattgac atcaacgatc tggataaata ctttcagaaa
    22801 ggcgaactga ttccggcgat tgcatttgac gtagatacaa aaacggttct gatgctggca
    22861 tacatgaaca aggaaagcct gcgtaagaca ttggaaaccg gttacacctg gtactggagc
    22921 cgttcccgtc aggagctgtg gaacaaaggc gcaaccagcg gtcatctgca aaaggtcatc
    22981 tccatttaca gcgactgcga tgatgacaca ctgctgctca atgtgaaaca gactggtgca
    23041 gcgtgtcata ccggctcgta cagctgcttt ttcaatgaga tgtacacaga cgattctacg
    23101 gctgaataaa atttagaaaa gcagtcacat ttttcaaaac ctccgaatac actgtagaga
    23161 agcatctatc agaaagctga taggcttaaa gcagcgcatc ttatgcacag ttttgtgcag
    23221 cgttgcctta acgtgttaaa ggaggttttt tctatgagcc aatatcaccc tctgccggaa
    23281 cactgtgcag atacggtacc gatcaaggtc aaccgtgtat ttgacagctg cagtgaccgg
    23341 gactgtttct ccaacattca gatcctcatg gacgggggag aactgccggc aaatgtcact
    23401 atggtgaaaa gccgctgtgt ccgtgtttcc gacatctgca tgaacatcga accagttccg
    23461 tttaaccggg gtttctactc gatcgactta acctacacat tccgggtaga acttatggcg
    23521 tatgaacgtg cctgcagcag tccggtgctg ctgaccggca ctgcctacgc cagcaagaac
    23581 gtgatcctgt acggcggcga aagcaacacg cagacattct tcagcagcgg tgaacagctg
    23641 ggcgacacca acgcctgctg tgaaaccatc aatcttccta ccgcagcagt acaggtggtt
    23701 gcacccattg cactggaggc acgcatcggt tctgtttgtg ccgcagacgg cacaacgccg
    23761 gttcgtactg ttattatgac gctggggctt ttctctgtgg tagaaatatt ccgtccggtg
    23821 accatcctgg ttcccactta cgaatacacc attcccacca aggaatgcca taccgatacg
    23881 gaatcaccct gcgctgtctt tgacaagatc cgttttccgg cggaagaatt ctcaccggga
    23941 acgctggatg ctatcccgaa ggaaaagacc tgcggctgcg gagaacccac agaatgcggc
    24001 gatacgccta catcggaata aagtgtaaca acgggcggct gattgtcgtc cgtttcttgt
    24061 ttcattgagg gtggaaaatg cgaaaacaag tataccaaaa aaggcgcgcc aggctgcgtt
    24121 cgctgctgat cttttgtact gctgcggcga tcctgcttgc actgctgctg cggacagacc
    24181 gcctgatccg tccggaactc tgcgcagtct gcgaaagcga gaccaaacaa ttcgccgccg
    24241 gcttaatggc agacagcgtt tccgaggttc tgcagcagga acagtttcag tacagcgact
    24301 ttgcggcgct gacctatgac aactccggaa atgtaactgc cgtggaaacc aaaacaaacc
    24361 agatcaacca tttgcagact gcgatctatc agagcgtgca gcacaacctg gagcaatgcc
    24421 gggatgcgtc actcaccgtt tctctcggaa ctgcatccgg cgtgtggctt tttgccggtc
    24481 acggaccgca ggtttcagtt cgccttttgc ctgttggtca cgcctcggta aaactaatca
    24541 gcgctctgga atccgcaggt gtcaaccaga cctgccacac gatccgggca gagatcactg
    24601 ccgagatacg ggcagccatc ccattttcag aggtaaccgc tgttgccaca tatgactgcc
    24661 tgctttccga aaccgtcatt gtgggaaatg tgccggaatc ctatctggaa ttcggcagca
    24721 gcgacacagt tccacaaaac caggatttta gagaataaaa catgaaatat ttgtaaaagg
    24781 tctagcatat gccctgtttt tgtggtataa tataaatgtg tgccaagtgg aagaatccaa
    24841 acggcagcac aaggagacgg aaacatggac gaaacaacag caaaacagcg cattgcagag
    24901 ctgaaacagc tgctggagcg ctataacgaa gaatattacg tgcaggacgc accgaccgta
    24961 acggactatg agtatgatat gctgatgcgg gaactggaga ccctggaagg ggaatatccg
    25021 cagtttgcag cagcagattc tcccacccgg cacgttggcg gcacagcatc ccgaacattt
    25081 gaaaaggtat ctcatgcagt acagatgatg agcctgcagg atgttttttc ctatgcggaa
    25141 atggatgcct ttgtgcagcg ctgccaggaa gaactggagc atccggcatt cagcgttgag
    25201 ccgaaaattg acggcttatc ggtatctttg gaatacacca atggcatttt cacccgtggt
    25261 tcgacacgtg gtgacggctt tgtgggggaa gatgtcaccg ccaatctaat gacgattccc
    25321 aacattccaa aacagctgaa agacgcaccg gcattcctgg aagtgcgtgg cgaagtctat
    25381 atgccacgga aaagctttgc cgcacttgta gaacagcagg aactggacgg agaaacgccg
    25441 gcaaagaatc cccggaatgc agctgccggt tctctgcgcc agaaaaacgc agctgttaca
    25501 gcaaagcgca gcctggacat ctggatcttt aacatccagc agatagacgg cgcatctctg
    25561 gaaacccaca ttgcatccct ggaatatctc aaaaagctgg gattccccac ggtgattccg
    25621 cacagcaagc cactgcaaac cgcagaggaa gtgcgtgcag aaattgcagc gatcggagac
    25681 gcaaagctgc attatgccta tgacacggac ggcgttgttg tgaaggtgga ttcttttgca
    25741 cagcgggaag aaatgggcgc tacgtcaaag gttccgaagt gggcagcggc gtttaagttt
    25801 ccgccggagg aaaaagagac cacacttcgt gagatcctgc taagcgtggg cagaaccggt
    25861 gtcatcacac cggtagcgat tttcgatccg gtacagcttg ccggaacaga agtctcccgt
    25921 gccacactgc acaaccagga ctttatcacg gaacgtgaca ttcggctcgg tgataccatc
    25981 gttgtccgaa aggcagggga gatcatcccg gaagtactgc gtgtcaaatc gcacctggac
    26041 gacgcagagc cgtatcactt gccggagcaa tgccctgtct gtcatacacc agcagtgcgt
    26101 gatccggatg aggcagcgct gcgctgtccc aatccagact gtccggcaca gcttatgaaa
    26161 cgcatgatcc attttgtgtc caaagacgcc atgaacattg acggtctcgg accgcagaac
    26221 ttagcggcac tgcaggcaaa tgatttggta cattccgtgg cagatctgta tgcactgaca
    26281 ccggaaaaac tgacaaaact ggatcgcttt gcagagaaat cggcagagaa cctggttcgt
    26341 gctattgcga catcaaagga aaactctctg gatcgtctga tctacgccct gggaattcgt
    26401 ggcatcggca gccgggcggc aaaactgctc tgtgagcatt tccccaccat ggatgcgctg
    26461 cttaatgcaa cagctgcgga gatccaggag atcgacggct ttggagaaac catggcagaa
    26521 agcgtggtct ctgcattggc agagtcgcat atgcgccagc tgatcgaaca tttacaggca
    26581 gccggctgca atatgactta tcatggaaca gccgtagagg acacacgctt tgccggcatg
    26641 acattcgttc tgacaggaac gctttccacc atgaagcgca gcgaggcaaa agaactgatc
    26701 ctgcggtacg gcggaaaggt cagcggcagc gtctcgaaaa agacttcgtt tgtcgttgcc
    26761 ggagaggctg ccggcagtaa gctggacaag gcaaatgagc tgggtatccc tgtactcagc
    26821 gaagatgaat ttcgtcaaaa aatcaatgag aattgatccg acagcatggc tttgacgctg
    26881 tgccgccgca attctcaagg aaaggatttt tatatatgaa aattgatatt gcacacattg
    26941 caaaactttc ccgacttcat atcgagccgg agaaaatgga caagttcgaa aaagatatgg
    27001 ctgccatcgt cgacatggta gaccgtctgc cggacgtgga agatgaactg acgctggacg
    27061 cagcgcatcc catgaaactg cgtgaggacg tggctgtgga acacaagttc cgccgggaag
    27121 agatgctggc aaacgcaccg gaagtacagt ccggctgtct tgtcgttccg aaaacggtag
    27181 aataaggagg aaatacacaa atgaagctgt ttgaaaaaaa tgcggcagaa ctgtctgcaa
    27241 tgctacaaaa caaagagtgc agtgcggttg aactgacaaa ggatgtcctg gacagaatcg
    27301 acgccaagga atctgcggta ggcgcttatg tgacacgctg cgaggattgc ctggaacagg
    27361 cagaggctgt ggacaatgcc cgtgcagccg gcgaggaact gcatccgctg gcaggcatcc
    27421 ccatcggcat caaggacaac atttccacaa agggcatccg cacgacctgt tcctccaaaa
    27481 tgctggaaaa ctatgttcct ccctttgatg caacggttat gcagccggtt cgcaaagctg
    27541 gcatggttat caccggtaaa atgaacatgg acgagttcgc catgggttct tctaccgaga
    27601 cttcttattt ccacactacg cacaatccgc ataacctgga ctgtgttccc ggcggttctt
    27661 ccggcggcag cgcagcagct gttgcagcag gtgaggcgat tctcaccctg ggttccgata
    27721 ccggcggatc tatccgtcag ccggcatctc tgtgcggcgt agttggtctg aaacccacat
    27781 acggcagcgt atcccgttac ggtctggtcg catttgcatc ctctctggat caaatcggtc
    27841 cgttcggtaa gtctgtcaag gatgtggcac tgctccagag cctgatccag ggacacgaca
    27901 acatggatgc cacttccgct taccggaatt atgccaacct gtatgagaat ctgaacggca
    27961 gtgtccaggg tctgcgcatc ggtgtcccga aggaatactt cggcgagggc attgacgcag
    28021 aagtcaagga tgcggtattt gctgccatca agctgctgga acagaacggc gcagtcatca
    28081 agaatatctc actgcccagc acagaatatg cgatcaacac ctactacatc atcgcatctg
    28141 ctgaggcatc ctccaacctg gctcgtttcg acggcgtgaa gtacggtttc cgtgctaaaa
    28201 attacgatgg tctgaccgat atgtacgaga aaaccagaag tgaaggtttc ggcgatgagg
    28261 tcaagcgccg catcatgctg ggtacatttg tgctgagttc cggtttctat gatgcttatt
    28321 ataaaaaggc aaagctggta cagcgccgca tcggacagga attcgctgcc gcattccagg
    28381 actgcgatgt gattctgacc ccgaccgcac cgtctgctgc atttaagatc ggcgaaaata
    28441 ttggcgatcc gctgaaaatg tactacagcg atgtctgcac cgttaccgta aacattgccg
    28501 gtctgccggc tgtcagcgtt ccatgcggaa agtctgcgtc cggtctgccg ctaggtatgc
    28561 agctgatcgg caacaagttt gcagaacaga caattctgaa cgccgcatat ggttatgaac
    28621 agctggtcgg cggttttcac gccattgcag aaacccttta agcaggagga cgaaacagat
    28681 gaaagattat gaaatggtcg ttggactgga agtccacgct gaattgaata caaaatccaa
    28741 aatttactgt aactgccgca atgcgttcgg tctggaagtc aacagccagg tctgcccggt
    28801 ctgcatgggt atgccgggaa cacttccgac cctgaatgaa aaggttgtgg agtatgcgat
    28861 caagatggga cacgcaatgc actgccagat caacggcgtg tgcaaacagg accgaaaaaa
    28921 ctacttctat ccggacctgc cgaaggcata ccagatctct caggcagaag taccgctttg
    28981 cggcaaaggc tatctggatg tcatggttga cggcaaaatg aaacgcatcg gcgtgacccg
    29041 tatccatatc gaagaggacg caggcaaact gctgcacgac gagtcgttct ccggctccct
    29101 ggtcgatctg aaccgctgcg gcgttccgct gatcgaaatt gtctctgagc cggatatccg
    29161 cagcagcgcc gaggcaaagg catacctgga gaccatcaaa tccattctcc agtatctgaa
    29221 catctctgac tgtaaaatgc aggaaggttc tatccgctgc gacgttaacg tttccgttcg
    29281 tgaaaagggt gcaaccgagt tcggcactcg ctgcgaaatg aagaacgtca acagctttag
    29341 cgcagccgtt cgtggcattg aatatgaggc aaagcgccag atcgaaattc tggaggcagg
    29401 cggcaccatt gcacaggaaa cccgccgctg ggacgatgcc aagggtatga gcattctgat
    29461 gcgtaccaag gaagatgcac aggactaccg ctatttcccg gaaccggatc ttctgacgat
    29521 cgttgtgccg gaagaaaaga tccaggcgct gaaagacagc attccggaac tgccgaatgt
    29581 caagctgcag cgttatgtct ttgacttcgg actgtctcag acagatgcct ctctgattgc
    29641 agaagacatc tcccgttcca agctgtttga tgcgtgcgtt gcactgggca actgcaagcc
    29701 gaagagcatc agcaactgga ttctggcaga cattgcgaaa tacaccaacg aaaccggaaa
    29761 gaccattgaa gaaactgctc tgacaccgga acatctgacc gctctgatcg aacaggtgga
    29821 aaagggcgtc atctccaacg catctgcaaa gaaggtcttt gctgtaattg tagaagagga
    29881 caaggatccc attgcgatca tcgaagaaaa gggcatgaaa cagaattccg ataccggttt
    29941 cctggaacag ctggcaaagg atgttctggc gcagaacgaa aagtcggtta ccgactataa
    30001 aaacggtaag accaatgcgc tgggctatct ggttggtcag tgcatgaaag catccaaggg
    30061 caaggcaaac ccgggcattg ttaaggaaat cgtaaccaag atgctgaacg cataaatttt
    30121 caaaagcaac tgctaaaaaa tcaaggcaac tgcatcacct gcagctgcct tgatttcaaa
    30181 atcgaatgac gtttcagtgc agttttacct gcggaaactc ttttgtgacg aatgccggca
    30241 gcggtgtctg gaccgtcaag ccgtttaggt ttgcgagaca ggaatcgctg tcggtttcta
    30301 attgcagctg atacgcccac aacagctggt gcttgcagtg cagcgcacgg ttttcccgca
    30361 tatttccgta tttggtgtcg cccagaatcg gcgcatgaat gtgagcaagg tgcgcacgaa
    30421 tctggtgcgt tctgcctgta atcagatcca ccgacagcag cgcatggcgc tcattgaatg
    30481 ccagtacttc atatgcggtg atgatctctt gaaatccttc ttccggcgtg tcacgaacag
    30541 tgaccgtatt gtgactggcg gatttcttgt gccacccata aagcgtagcc tttcgttctt
    30601 tgggaacgcc aactgtaata cacaggtact ttttatgtac cttgttttcc cgaatcaacc
    30661 ggttcacttc ccggagcgcc gcagctgttt tggcggcaat gacaatacca ccggtgtttc
    30721 gatccagacg gttacagagc gcaggcgtaa aactttgttc ctgctccgga tgataggctc
    30781 cggttcggta aagataatgg agaacacggt tgaccagcgt atccgggatc tggcggctgt
    30841 cacagtgtac cacaagcccg accggcttgt ttaccaccaa gatctgcgca tcctcataaa
    30901 gaatgtccag ctgatccggt gcctgcataa attccggtat gccgctgcgt tcttcctctg
    30961 tcttgattgt aaagtatgag tcgctgacaa acagcgtcac tacatcacca gtcaccagac
    31021 gctgannnnn nnnnntccgg ttcaccttga tatgctttgt gcgaatccac ttgtagcaga
    31081 gactcttcgg caattccggc agggttttct gcaaaaagcg atccagccgc tggtcgctgt
    31141 cattttcctg aatgcaaaat tctttcatat tttcaatttg ctgcaccgtg cagcattcct
    31201 ttcgtaaatt ataacgtgca gccgaaaatc agactaacac tgatattgct attatatcat
    31261 attttcatcg aaaagaaaag cttttctttc ggcagatcgg aggtcatctc atggaacagt
    31321 caaaaaaggg acagcatcac ccgaatgcag ctgcggacaa gcagagtact tcagaactgt
    31381 ccgctgttcc aaagccaaag cataaacact acggtcatcg ggagagaatg cgtactcgat
    31441 tccagaagag cggactggaa ggctttcagc cgcatgaaat actggaactg ctgctgttct
    31501 acgctctgcc gagagtagac accaatccga tcgctcacga tctgatagac acattccact
    31561 ccctgtccgg tgtgctggac gcagacatcc aggatttgaa acacgtcaag ggaatcagcg
    31621 aaaatgcagc ggtgtttctg aaactgctgc ccgaactttt tcagcagtac cagctggata
    31681 agctccggga acacgctgca ctgaacacca cagaaaagct gagcgcctac atagcggcaa
    31741 agctgtccga tgcagtggaa gaaaaagcac ttttgctctg tctggatgcg catttgcacc
    31801 tgctccactg tgaaacgatc tctgccggga cagcgcagag taccatactg gacacccacc
    31861 gcattgtgga atgcgctatc cggatgcgct gcagccgtat tgttctggcg cacaatcatc
    31921 cccagggaaa agcctgcttt tccgatgcgg acctgttcgc cacccagcag ctgcggcgtg
    31981 tgctggacag tatgcagatc cagctgttgg atcacatcgt gctgggaaaa tctggcgaaa
    32041 ccatctccat gcggcaggtc atggggtgga gtgaaatata aggagggatt ccatggatca
    32101 ttttgaactg cacgcaccgt tcgaaccaac cggcgaccag ccggaggcaa ttgcaaaact
    32161 agtgcagggg ataaaagacg gcgatcacga acagacgctt ttgggcgtta ccggttccgg
    32221 aaaaacatac accgttgcca atgtcattgc acaggtgaac aagcctactc tggtgctggc
    32281 acataacaag atactggcag cacagctctg ttcggagttt cgggaattct tcccgaacaa
    32341 cgctgtcgag tatttcgtct catattacga ctattatcag ccggaggcgt atattcccag
    32401 cacagacagc tacattgaaa aagactcatc cattaacgat gaaatcgaca aaatgcgtca
    32461 cagcgcaaca gcagcgctgg cagagcgaaa agatgttatc attgtggcaa gcgtttcctg
    32521 tatctactcn nnnnnnnnnc tctatgatgg tctccatccg tcccggcatg gaaaagccca
    32581 gagatcagct aatcgacgaa ctggtagcga ttcagtatga gagaaacgac atcaactttg
    32641 tccgaaataa gtttcgggtg cgtggcgatg tggtggagat ctttccggca tcctccacag
    32701 ataaggcaat ccgtatagaa tatttcggag atgaaatcga ccgcatttgt gagatcaatg
    32761 tcgtctccgg tgaagttact gccatcatgc agtatgcggc gattttgccg gcatctcact
    32821 atgtggtagg cagcgaaaaa gttacaaaag ccgtcacgga gattcgccgg gagatgttgg
    32881 aacgagtggc atggttcacc gatcatcaca agctgattga ggcacagcgt attcagcagc
    32941 ggacacagta cgacatggag atgctggaag agatcggctt ttgcagcggc attgagaact
    33001 attcccgtat tctcgccggc agaccgccgg ggagtgtgcc gtacactctg ctggatcact
    33061 tcccggaaga ctttctcttg atcgtcgatg aaagccatgt cacactgccg caagtacgtg
    33121 ccatgtatgc cggagaccgt gccagaaaac agaccctggt ggactacgga ttccggctgc
    33181 ccagcgcatt cgacaaccgg ccgctgaact tcgaagagtt ttacagtcat gtaaaccagg
    33241 caatctacgt cagtgctaca ccaggaccgt ttgagaaaga acactctgta caaacggtag
    33301 agcaggtgat tcgtccgacg gggctgctgg atcctctgat tacagtgcgt ccggtggacg
    33361 gtcagatcga agatcttttc tcagaaatca acatccgtgc agaaaaaaac gaacgtgttc
    33421 tcgtcaccac actgacaaag aaaatggcgg aagatctgac cgcctatctg gaacgcatgg
    33481 gcgtccgtgt ccgctatctg caccatgata ttgatacggt agaacgtatg gagatcatcc
    33541 gggatcttcg cctgggcgag tttgatgtac tggtaggcat taacctgctg cgtgaaggac
    33601 tggacattcc ggaagtttct ctggtggcaa ttctggacgc agacaaagaa gggttcctgc
    33661 gcagtgaaac ctctatgatc cagaccatcg gccgtgcagc ccggaatgca aacggacagg
    33721 tcatcatgta cgcagatgaa gtgacaggtt ccatggagcg tgccatcaca gaaacagagc
    33781 gtcgccgtgc caagcagatg gcatataacc aggaacacgg tattatcccg aaaaccattc
    33841 agaaggatgt gcgggatgtc atcgaaatca cttccaaggc agaagtggaa aagaaaacat
    33901 ccggcaaaaa actgcgtggc aaggaaaagc gtgcgcttat tgaacagctg accgcacaaa
    33961 tgaaagaggc tgcaaagctg ctggaattcg aacacgctgc atacctgcgt gacaagatca
    34021 aagaactgga gg
//
LOCUS       DAWENO010000005        28800 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_669_length_28800_cov_10.176170, whole genome shotgun sequence.
ACCESSION   DAWENO010000005 DAWENO010000000
VERSION     DAWENO010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 28800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28800
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_669_length_28800_cov_10.176170"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8857..8866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11469..11478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15371..15380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17154..17163
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6716 a         7440 c         8618 g         5986 t
ORIGIN      
        1 tgatgccgat cgaggatact atgaccatct ctggccgtgg taccgtcgtt accggtcgtg
       61 tagagcgcgg aaagctgaac gttaacgagc cggttgaaat cgttggtctg tctgaagaaa
      121 agctgaacac cgttgtaact ggtctggaaa tgttccgtaa gtctctggac ttcgcagagg
      181 ctggtgataa cgttggcgca ctgctgcgtg gtatcactcg tgaccaggtt gagcgtggtc
      241 aggtactgtg caagccgggt tccattcacc cgcacaccaa gttcaagggt caggtttacg
      301 ttctgaagaa ggaagaaggc ggtcgtcaca caccgttctt caacaactac agaccgcagt
      361 tctatttcag aacaactgac gtaaccggta tcatcactct gccggcagac gttgaaatgt
      421 gcacacctgg cgataacgtt gaaatggacg ttgagctgat tcagccgatc gctattgaag
      481 aaggtctgcg tttcgctatc cgtgagggtg gtagaaccgt aggttctggt gtcgttacag
      541 caattaacga ataagtaaat cgcatctgat gcgtttttca aaaggggacg agcttcggct
      601 cgtccttttt ttgttgcctg gattttgtgc aaggtgtcta aaaaaaatac gttttttcgg
      661 gcatattttt cccgttttct ttcgctgcat tcttgcaatt tcaccgtaga tacggtataa
      721 taataacata gcggtgaaaa gtggggagaa ttccctaagc gtggagaaac tttccatgcc
      781 agaaccgcag ctttttgcag aaaggcggca tcgaatatga cttccttgat gggaacatat
      841 taccataata tagatgccaa agggcgtatg aatttcccaa ccaaactgcg ggaagtgtta
      901 gggacaacat tttatgtgac aaaaggactg gatcagccct gtctgacggt ctattccgag
      961 acggagtggg agcgccttgc ggcaaaggtt gcagcgatcc cggagtccaa aggcggacag
     1021 atccggcgct ggctcttttc cggggcaggc gttctgacgc ctgacaagca ggggcgtgtg
     1081 ctgatcccgc aggatctgcg cagttttgcc ggtttggaaa aagatgttgt cgtgatcggc
     1141 gcaaacaata aagcggaaat ctgggaaaaa tctcgttggg aggcgctgaa cgccgctttc
     1201 gatacccaga aaatgctgga aaccttagag gaaatcggat tttaggtatg gaatttgtac
     1261 acattcccgt gctgctgcac gaggtgctgg atgggctggc aattcgcccg gacggcattt
     1321 atgtggacgg caccgtcggc ggcgccggac actccacgga aattgcaaaa cgtctgacgg
     1381 acggcggcag gctcattggc tttgaccggg accccgacgc agttgccgtg gcaacggagc
     1441 ggctcgcacc ttatccggca acggtggtac accacaatta tgatgaaatg cagacggtgc
     1501 tgacggagat gggcgttgtc ccggtggacg gcgtgctgct ggatctgggc gtttcgtccc
     1561 accagctgga cgaggattcc cgtgggtttt cctatcacca cgatgccccc ttggatatgc
     1621 gtatgagcca gaccggcatg acggcggaag aactggtgaa tacggtttcg gaacaggagc
     1681 tttcccggat cttgtatacc tatggggaag agtccttcgc ccggagcatc gcccggaata
     1741 ttgtgcgggc aagagaggaa aagccgatcc acaccacgct ggaactggcg gaaatcatca
     1801 aaagcagcgt accgcaaaag gtgcgccgga tgaaaaatcc ctgcaaaaaa tccttccagg
     1861 cgatccgcat tgcggtgaac ggggagtttg aacatctgga aaacgggctg aaagcggcgt
     1921 tttctgtgct gagacccggc ggcagactgg cagtcattac cttccattcg ctggaagacc
     1981 gtatggtcaa gcggcagttt gcccagtggt gccaaggctg tatttgtccg ccggactttc
     2041 cccagtgcgt ctgcggcaga acgccggagg caaagctggt ttcccgaaag cctatcgcac
     2101 ccagtgcaga agaactggca cagaacagcc gcagccgcag tgcgaaactg cgggttctgg
     2161 aaaggagtcg ctgacattca tggcggatca gaaaaaatca accgttccgg aagaaccggc
     2221 ggcacaggaa gaaaaggcac cgtcttcggg ctttttcgtg ttccgggtca gctccggaaa
     2281 gcgtggcgct gcgctgaaag tgttcattgc cggcattctg gcactgctga tgttcagcgt
     2341 cattatcaac agctatgtgc agctgaccga ggtctattcg tccatttcag aggcgaacgc
     2401 agaactgaac accctgcgga gcgaaaatgt gcggatgcag accgagctgg agggcaaggc
     2461 gtccatcagc aatatcaagg attatgcgga acaacacctg ggactccaga aactggatca
     2521 gtcccagatc caatatatcg acattcagac agaggatacc gtggagatcg acggagaaga
     2581 gcagaacatt tttgtccggc tcaagcacaa gtttgaggat attgtggcat atctccgagg
     2641 ctgatgccca tacaatgaga tgaggcgcag cccaggcata acggtgtcct gtcactcatg
     2701 ctggctatcg gtatgtcccg gaacagacga gggaaggggc aatgctgtta gttcacataa
     2761 agttaccgag taggaatgca cacagtcggg ctgacaatca tatggctgcc ggacaggtga
     2821 ccgttgcaag gcggtgtccc cattcctgca tacgttttgc agcaagggaa tctgttgctg
     2881 caaaatggga aattcccgtc gctgtggaaa tatgcccgga tgacggttga aacaggcggg
     2941 gtaatggcaa cgattccctg cctgattcta ttatataggc atggacgcag gcattgcgct
     3001 ctgtagaata caccgggaac agaggaaaat tgcagaaaaa acggaggtgt gccgaacgtg
     3061 cgcatgataa atgaatccaa ggaaaagaaa aaaaatccgg aaaagacacc acgttctgcg
     3121 tccgcagtga tgcgccgccg tgcagcggtc agcatgactg tcctggtggt aatgggcgca
     3181 gcagttgccg gaaagctgtt caaggtctcc atcgtggaca attccaagta tgagacgctg
     3241 gcgaacaact atcacttcgg gaccatgacc ctggacgcaa accgtggcgc catctatgat
     3301 gccaacggaa cggcgctggc gtggagcgca acggtatata atatatatgt tgacccgaaa
     3361 ttgtatcggg atgagatcaa ggagatcgaa aagtccaacg acaagaaaaa atctgcggca
     3421 gaggaaaagg gggaacaggc ggcaaacctg gtggacgtga cccagctgga acagtccatc
     3481 gtctctttcc tctcggaaaa gctggagatc gacgcatcca aggtgcagga ggcgcttgcc
     3541 aaggacggca gatactgcgt cctgcaaacc caggtggaaa agaacacggc agacgagatt
     3601 accacatatt ttgataagct gaagctcacc tttgtgggaa cagaggcgac cacacggcgg
     3661 tattatcccc agaatgaact ggcggcggca gtcatcggtt ttaccaacgg tgacggcgac
     3721 ggacagtacg gtctggaata tcagtatgac gaatacctgt ccggcgtaga cggcagaatc
     3781 atttccgcac aggcggcaaa cggcgaggag atgccctatc ggtattccac cacctatgag
     3841 gcgaaggacg gcgcatccct gtatctgacg ctggattcca ccatgcagta ctatctggaa
     3901 aagagtatgt ccgagatggt ggaaaagttc gaggtcaatg accgtgccac cggcattatc
     3961 atgaacccga aaaccggcgc tatctacgct atggcgacct gtccttcctt cgacctgaac
     4021 aacccgtcgg agatatacga cccggtggtt gcggcgcagc tgaaagagct gccggagagc
     4081 gaataccagg acgcatacct ggaggcaaga gaaaagcagt ggcggaacaa ggcaatcagt
     4141 gagatcaacc cgccgggttc taccattaag atcttcacat ctgcgtcggc atttgaggaa
     4201 aatctgatcg atctgaacac cgacagcttt tattgcagcg gctctttgaa ggtacaggat
     4261 ctggacatcg gctgctcgct gcgttccggt cacggcgcac agacctttac ccaggcgctg
     4321 accaattcct gcaacccggc gtttatggag atcggtctgc ggctgggcgt gcagaagttc
     4381 agctattatc tccagggctt tgggctgaca gaaaagaccg gcatcgacct gccgggcgaa
     4441 gttgccagcg tatgcgtacc ggaagacaag atgacacagg tcgatctggc atccggcgca
     4501 ttcggacaga ccaatgaaac cactgcactg gaactgatta ccgcttatgc ggcagccatc
     4561 aacggcggct atgtggtaca gccctatgtg gtagaccata tcgtagacag cagcggcaac
     4621 acagtcctgc aaaataccac tactgaaaag cggcagatca tctcggaaga aacttcgaaa
     4681 acggtacggg aacagctgga ggcagtagtt tccggcaacc cgtcgcataa cgcctatatc
     4741 cagggctacc gcatcggcgg taagtccggt acggcagaaa tccgtgccac ccgggacatc
     4801 gaagatgact atgtggcatc gtactgctgc tttgcgccgg cagatgatcc ggaactcatt
     4861 atgctgatcc aggcggacta tccgaatccg gagatcggct actacggctc gaaggtcgta
     4921 acgccttatg cgcaggagat catggaggag atactgccgt atatgggatt ctatccggaa
     4981 tataccgacg aagaggcaaa ggaaatgaat gttgctgttc cgctgctcca ggatgccacg
     5041 atcgaaaatg cgcaggcaac gctggaacag atgggactga cctatgaagt tgtgggcagc
     5101 ggctcgacag tagtttccca gtcgcctacc accggcacaa gcgttgccaa gggcggaaag
     5161 gtgctgctgt atacagagca gaccaccaca cgtgacctgg tcaccgtgcc gaatctgatc
     5221 ggcatgaccg catcccaggc aaacgagacc ctgtcctatt acgggctgaa ctatgcgctg
     5281 atgggtgcgt ccgcaagcag tgagggcgca ctggtcaaga gccagtctgt cagggaaggc
     5341 acacagctac agcgtggctc ttccatcaca ctgaccctgt ccgccggaga ggcgaccgat
     5401 taaagacagc gccgcacagg ttttaggcgg cactgtccgg agttcatacc ttcacacaca
     5461 gaaaggatga gagaaatgca gttatatgcg cttttggaaa acaatgcgaa aaccagtctg
     5521 ccggatatgg agatcaccgg actgacagac gattccagaa aggtacagcc gggaatgctg
     5581 ttcgcctgca tccgtggaga acgctttgac ggacacacgg cggcgaaaga cgtgctggaa
     5641 cgtggtgcgg cagcggtgct ggctgaccac gacctggggc tgggcgaccg gcagatcctg
     5701 acagaggaca cccgtgcgct ctacggaaag ctctgcgccg catggttcgg tcatccggag
     5761 cggaagatgc gtttcatcgg cgtcaccgga accaatggca agaccaccat caccaatctc
     5821 atcaagcaca ttctgacgga aaacggacac aaggtgggac tgatcggcac gatccagaac
     5881 gagatcggag acgagattat ccacacggac aataccaccc cgttcgtcta tgatctgatg
     5941 gcgctgtatg ccagaatggc agaggcagga tgtgacgatg tggtcatgga ggtgtcctcg
     6001 ttcggtctgg tgcagcagcg catcggcgag acccatttcg cagcagctgc gttcaccaat
     6061 ctgacccagg atcacctgga ttatcacggc acgatggaaa actattacca ggcaaagaaa
     6121 ctgctgttcc agcggtgtga tactgccatt attgatacag atgacgaata cggcaagcga
     6181 ctgtacggcg aagtgacctg tcccaggatc tcttacggca gcagcagcca ggcggatttc
     6241 tatgcagacg ccatcaagct ccgggcagac ggcacgagct tctggctgtg ctgcggcgga
     6301 aagtcctatc cggtgaacct gcatctgacc ggaatgttca acgtgtccaa cgccctggcg
     6361 gcggtggcat cctgccatgc cgtggggctg tccatgcggg agatccgcac ggcgctggag
     6421 acctgcaccg gtgtccgggg acgctgtgag gtgatcccca ccggcagaga ctttaccgtg
     6481 atctgcgact atgcccacac gcccgatgca gtggaaaaca tcctgcggag cgtaaaggaa
     6541 tacaccaagg gcagactgat ctgtctgttc ggctgcggcg gcaaccggga tgccacaaag
     6601 cgtccgaaga tggcgaaggc tgcggcaaag tatgcggact ttctggtcat cacctcggat
     6661 aatcccagag atgaagagcc ggaggcgatc gttgccgaca tcctcaccgg actggagggc
     6721 agcgatgtgc cgtatgaggt ggtgatcgac cggaaggatg ccattctgcg gagtatgcag
     6781 atggcacagc cgggcgatgt gatcgttctc gccggaaagg gtcacgagga ctatcaggta
     6841 ttgaaaaacg gtgtacatat tcatatggac gaacgggaga tcgtgcgtga ctgtctgccg
     6901 cttgttccgg agcgataagg aaactggggg agaatgcgat gaaaccttgg atgttgtcag
     6961 aattgaaaga tgtgctgggc tgtgatgtgc cgacagaggc agagatcacc tgcgtcagca
     7021 ccgatacccg tgacctgccg cagggctgtc tgttcgtggc gctgcgtggg gaaaagttcg
     7081 atgcgcacga tttcgtgaaa caggcgatcg gggcaggcgc cgtggctgcg gtcacggaaa
     7141 agcccatcga gggctgtccg tgcctggtgg taaagcatac cggcagagcg ctgctgcaaa
     7201 ttgcaaggtt ttatcgggag aagttccacc ccacgttggt gggcatcacc ggcagtgtgg
     7261 gaaagacgac caccaaggag atgatcgcct gcgtgctgga atcccagtat gccaccctga
     7321 aaacccaggg gaacttcaac aacgaggtcg gtctccccaa gaccctgttc ggtctggagg
     7381 acagccacga ggcagccgtc attgaaatgg gaatgtccca cttcggcgag atcagccgcc
     7441 tttcccggac ggcagagccg tgcatcggcg taatcaccaa catcggctat tcgcacattg
     7501 aaaacctcaa gacacaggag ggcatccgcc aggcaaagct ggagatccag gacggcatgg
     7561 cagaggatgc gccgctggtg gtaaacggcg acgatcccct gcttgcgcct ttgaaaaagg
     7621 aactgtcccg tccggtcatt acctacggaa tgcacagcga taaggcagat gtccgtgccg
     7681 tggagatcca gcgccaggcg cagtccaccg ccttttccat ccggacagag gacggaaaga
     7741 cctatccggt ggaactgcct tgtgccggcg accacaatgt gatgaatgcg ctggcggcat
     7801 tctgtgtggg acggctggca ggcattgcgc cggagcagat ctgcaaggcg ctggcacagt
     7861 atcacaccgt gggactgcgc cagaatattt accgcagggg cgcatatacc attattgcgg
     7921 actgttacaa cgccagcccg gactccatga aggcggcgct gaccgtgctg cgtgacctgc
     7981 cctgtaaggg cagacgggtc gctgtgctgg gcgatatgct ggaactgggc gagatgtccc
     8041 gtcagctcca tacgctggta ggcgatatgg cggcaaacag cgaggtggac gctctgttct
     8101 gtttcggaaa ggaatcgttt tatctggcac agcgtgctgc catgcaggaa gtcgctgtgt
     8161 atcataccga ggatcgccgg gagctgtgcc gtgtgatccg gcagtatctc cgtccgggtg
     8221 atgtgatctt attcaaggcg agccgaggga tgcacctgga ggattgtatt gacgtaattt
     8281 ttgagcagga ggaggaaacg gatcatgccg ttctggatta acattctcgt aggattgctg
     8341 gcgtttgtca ttgccgcact gggcggaaag gcgctgctgc cgtggctgca caagctgaaa
     8401 ttcgggcagc ccatcaaggt cgacttcggt cccagctggc acgcaaacaa gcagggaacc
     8461 cccaccatgg gcggcatcct gttcatcgtg ggcagcatcg tggcgaccgg cgtgggctat
     8521 gtgctgtacc gtctgttcgg catgactgac ttcgtctccc cgttcagcga ccgtgaggcg
     8581 ctgcgtgtgc tgagctgtct gctgttctgc ctgctgttcg gtgtcatcgg ctttatcgac
     8641 gacttcgcca aggttgccaa gaaacagaac gagggtctga aacccatgca gaagatcgtt
     8701 ttgcaggtgg tggctgcggt gctgtggctg gcggcaatgt actggctggg cgaccgtgac
     8761 accaccatcg ggctgggatt cacctccttc gacatcaagt ggttctacta tccgctgatg
     8821 gtgctggtca tcctgtatct caccaatgcg gtcaatnnnn nnnnnngttt gtcgtctttt
     8881 tgctggtggg caccaccaat gcggtcaatc tgaccgacgg cgtagacgga ctctgcggca
     8941 cggtgacgct ggtggcaatg ctcatcttca ccctggtttg cagcaagctc cagatggagg
     9001 actatacgct gtataccatg gcgctggcag gcggctgtct gggctttctg gtgtggaatc
     9061 tccacccggc aaagtgcttt atgggtgaca ccggttccat gtacctgggt gcctctgtgg
     9121 cagccatcgg tctggcgacc caccagcacc tcaccatgat cctggtggcg ctggtgtata
     9181 tcctggaggc gctttccgtt atgatccagg tgacctattt caagtatacc aagaagaagt
     9241 acggcgaagg ccgccgtatc ttcaagatga cgccgatcca ccatcacttc gagatgagcg
     9301 gatttagcga atataagatc gtgattacat tttctctgtt cggtgcgatc gccggactgt
     9361 gcggactgct gctggtgatc ttataagcag cagaaccaaa ggaggttgca tatggcagaa
     9421 ccaaaagttg ccggaacaaa aaagaagatg cggttcagcc ttccgctgtg gcagcgcaat
     9481 gtgcggtacg gcgaagtgga tctggcgttc tttctcatcg tgatcgccct gctggtcatg
     9541 ggcatcgtca tgatgttttc cgccagctat gcctgggcga tcgcagaagg acgtgacggc
     9601 acatattatg ctgtcagcca gatcaaaaac gcagtggtgg gactggtcat catgtgtgtg
     9661 ctgtgtctca tcgactatca ttttctccag aagaaattca ttgccgtgtg tgcctatgtc
     9721 ctgcccatcc tgctgctgat tatggtattt gtgccgggca tcggcgtcac ggacggcggt
     9781 gcgacccgat ggatcgccat tggcagcttt aacttccagc cgtctgaggt catgaagatc
     9841 ggcattgtca ttttcttttc ctatgtgatc gaaaagaact atgccaatat gcggtcgttc
     9901 cgcaagggtc tgctgccgct gctgtttgcc atggctgtgc cggcggcgct gctcatcaag
     9961 gagccgcacc tttccgccac cgttctgatt gccatgacct gcatcattct gatcttcgtg
    10021 ggcggcgcag atatcaagca gtttctgatc ctgggcgctg tggcgctggg tggggcgctg
    10081 ctcatcggct ttttgatgtc cggacactat gagtatatcg gaaagcgtat cgcctcctgg
    10141 cgagacccgt tctccgacag cctgggcgac acctggcaga cctgtcagtc tctgattgcc
    10201 attggctcgg gcggactgtt cggtctgggt ctgggcgaat cccggcagaa gtatctgtat
    10261 ctgccggaga cgaaaaacga cttcgtcttt tccattgtct gcgaagagct tggtctggtg
    10321 ggagcgctga ctgtggtgct gctttttgcg ctgctggtca tccgtggatt ccacatcgcc
    10381 atgcacgccc gtgacaagtt cgggatgctg gtggcgatcg gtctgacctc gcacatcggc
    10441 ctgcaggcat ttttcaacat tgcagttgtc agcaatctga ttccgaatac tggtatcagc
    10501 ctgccgtttt tcagctacgg cggtacggcg ctgatattac agctggcaga aatgggcatc
    10561 gttctgaaca tctcccgtgt gcggtatcgt gtgggtgcaa aagcaccggc ggcaaagccg
    10621 gcagaggaga ccacggaaca gctccaggga ggtaacgcat gaaagtatta ttggcaggcg
    10681 gcggcacggg cggtcatatc aacccggcgc tcgccattgc atccattatc aaacagcatg
    10741 acccctcggc agagttcctc tttgtgggaa cgccgaacgg catggaggct cgccttgtcc
    10801 cggcggcagg ctatgccctg gagaccatgc acgttgccgg atttcagcgc aagctgaact
    10861 ggaagaacat caagcggaac tgcagggcgg cgtattatct ggcaacatcc cagcaccgtg
    10921 ccaagcagat cttgaaacag ttccagccgg acattgccat cggcacaggc ggctatgtgg
    10981 caggaccgat tctccacgct gcggcgcata tgggcattcc ctgcgccatt cacgagcaga
    11041 acgcattccc cggcgttacc aataagatgc tggcaaagga agtcgatcac gtgatgctca
    11101 ccgtggaaaa ggcgctggat tatctccacc cggactgtgc ctataccgtc accggactcc
    11161 cggtgcgtgg cggcatcgcc ggaatggaca gagccgcagc ccggaaaaag ctgggctttg
    11221 atgacggaat gtgcatcctg tccttcggcg gcagtctggg tgcaggctgc atcaatgagg
    11281 caatggcaaa ggtgattccg tggcacacgg aaaagggtat ggcgatcaac catatccacg
    11341 gctacggcgg catgggaaag gaatctttcc ccaaagccat ggcagatgcc ggcatcccca
    11401 tgaagagcga ccggctgcgc attacggaat acatcaatga tatggatgtg tgtctggcgg
    11461 cagcagatnn nnnnnnnngc cgctccggtg cgtccacgct ggcagagctg gaggcagccg
    11521 gaagaggttc tattctgatc ccgtcgccca tcgtgacagg caatcaccag ctgcacaacg
    11581 ccaatgtgct gggagacgcc ggcgctgcta tcgtcattga gcagaaggat gcgtccccgg
    11641 agcggatcat ccaggagatc gaaaagctgt accaggaccg cagccgtctc acggagatgg
    11701 ggcagaacgc caaggcgctg gagatctccg acacggatga gcgtatctgg aatgtggtct
    11761 ctgcgctgta tcaaaagaca aaataaaccc ggtttcacca attttgccga aaccgcatac
    11821 actgaaataa aaagaggtga tgcggattgc aaaaactggt gatcgaaggc ggcataccgc
    11881 tggagggtga gatctttctg cacggggcga aaaacagcgc cctgccgatt ctggcgggga
    11941 cgctgctctg tgacggcgag acgactttgg aaaattgccc caggctgacg gatgtatttg
    12001 ccgcctgccg aattctgacc catctgggct gccgctgtat gatacaggaa cacacggtga
    12061 cggtgcaggt cagagatctg cgctcggata ccattccgga ggcgctgatg caggagatgc
    12121 gctcgtccat catttttctg ggcgctgtgc tggggcgttc cggcagctgc cacctgtcct
    12181 atcccggcgg ctgtgagttg ggtccccgtc ccattgatat gcacctggat gccctgcggc
    12241 agatgggcgc acatttttct gtacatgggg gacagctgga ttgcaccgca ccggagggac
    12301 tgcacggcgc agccatacac ctgccgttcc cttcggtggg agcgacagaa aacgtgatgc
    12361 tggcggcggt gctggcagag ggcagaacgg tgctgcacaa tgcggcgaga gagccggaga
    12421 tctgcgacct ggcggcattc ctgcgcacct gcggcgcaga catcgccgga gacggggaaa
    12481 gcactctggt catccgtggc gtggaacggt tgaaaggcgg cagatatgcc attatgcccg
    12541 accgcattgc cggcgtgacc tatctgggtg cggcggcgat caccggcggt acagtgtgcc
    12601 tgcgtggggt gaacacccag cccatggaga atatgctccc ggtgctggaa cagaccggct
    12661 gccgggtatt tcccgaaccg gatcggctgc acttgcttgc accacgccgg ctgaaagcgg
    12721 tgccgtctat ccgtaccatg ccgcatcccg gctttcccac agatgcccag gcgatcttta
    12781 tggcgatgct gagtctggcg aacggggtca gcgtcctgga agagaatatc ttcgagaacc
    12841 gctaccgtca tgtggatgcc ctggtgaaaa tgggggcaaa gatccacgtg tccggcagga
    12901 tcgccgtggt caccggggtg cgctcgctga gcggtgcatc ggtggcggca acagatctgc
    12961 gtggcggcgc agccatggtg cttgccgggc tgggcgcaca gggaaaaaca gaggtgcggc
    13021 agatctccca catcgaccgg ggatacgagt cgatcgagac ggtgctgcaa ggtgtgggcg
    13081 caaggatcac acgggtggaa tgaacttttc agaaaggaaa tgaggagctg aaacgatgca
    13141 ggatgtggaa aaaacagaga tccagagcca gacgagtccc aagcgcagac ggcgcagaaa
    13201 ccggatgcgt ccgctgtacg ggctgttggt ggcggcgctg gtgatcggtg tgggcgtttc
    13261 cctttcaatg acagtgtttt tcaacattgc gactattgaa atcgcaggcg atgcgcccca
    13321 atacacggcg gaggacattg cggatgcttc cggcgtacat accggtgaca atatgatgcg
    13381 gctcaaccgg gatgaggtgg agcagaacat cctggcacgg atggtgttcg tggacagcgt
    13441 ttccattcag aaaaactttc ccgacaagct ggtcattacc gtgacaccaa gcacgccggc
    13501 gtttaatgtg acggattcca gcggaacgct ccaggtcagc gcatccggaa agatcctgaa
    13561 aaacggtccg gacgccgatc ctgcgcttcc caccatcacc ggatttgaaa ctgctgtccg
    13621 ggagccggga cagatgctgg catccaagga cgaacagaag gataagatct tccaggccat
    13681 tgccgcacgt gtggcgaagg ggctggactg tccgctgaca gcggtggatc tgacggataa
    13741 gtatgacatc acgctgacct ttgacggcag agtggcgttt tcactgggaa actggggtga
    13801 tatggactat aagatcaccc tggcggagac tgtcctggga cagctcgctc cggacaaggt
    13861 gggatacctc accatggtgg gcgaccacca gtgttcctat cgggacaagg atgccgtgga
    13921 gcagcagacc actgcgccgc tccagaccat ggcgaccgat gaaaacggcg accccgtgac
    13981 ggagacagac gaaaacggca atgctgtaac gaccgaaact gagacaacga ccactgcggc
    14041 ggcatggcaa taatgggttt ttctttgaaa ttctgtatga aaatgtgaat ttggcacgga
    14101 tttttcccga aaatcttgtg ggaaagggaa aaacgtgatt tatttcaaaa aaaagcttgc
    14161 aatgccggcg aaaatgtgtt aaaataataa gtgtattttt agagatggac tccgtctgtc
    14221 cagaggacgg tgcggatcgt atacatggcg ctccgaaaag ccgggacatt tccgggaatt
    14281 cggcgtggtc tatagcttgt gagagaagtt cttgtaggag gaaatgttga gatgactgat
    14341 tttatctatg aagatgcgtt tgatcccgat gtaaatatta aggtgatcgg tgtcggcggc
    14401 ggcggcggaa acgcgctgaa ctgcatggca tcttcggatg tacatgacat tgagtatatt
    14461 gcagtcaata ccgatgcaaa ggcactgaat aattccaagg caaccaccaa gatccagatc
    14521 ggtgcaaagc tcaccaaggg tcgtggcgca ggcaacaagc cggaagtcgg cgagaagagt
    14581 gccgaagaaa acagagatga gatcgaggca gcactgaagg gtgcagatat ggtatttatc
    14641 actgccggca tgggcggcgg taccggtacc ggcgcagcac ctgtggttgc acgtctggca
    14701 caggaggcag gcatcctgac cgttgcagtg gttaccaagc cgttcgcatt tgagcgtgag
    14761 cagaagatgc tccaggcaga aaaaggtatc tctgagctgc ggaagtatgt agactctctg
    14821 atcgtgatcc cgaacgaaaa gctgctggag ggtctggaca agccgctgac catgcgggaa
    14881 tccttcgccc tggcagatga tgtcctcaag accggtgtca agagcatctc cgatctgatt
    14941 gtagaagagg gctatatcaa cctggacttc gcagacgttt ccaccatcat gaagggtgca
    15001 ggctatgcac acatggcgat cggtcacggc tccggcaagg acaaggcaaa ggaggctgca
    15061 aacgcagtta tcgcaagccc gctgctggaa acctctatcg ctggtgcaaa gcgcctgttg
    15121 atcaacattg caatgagcga agatatcctg tcctccgacg tggatatcgc aaccaagatg
    15181 attaccgaca aggcagcaga cgatgtcgag ttcatcttcg gtactgcatt caaggagaat
    15241 atgcaggatg agatggtaat tactgtgatc gctgctggtt tcgatatggt tgacccggat
    15301 gctccggcag aggaagagac cgcagaagag ccgattccgg tagataatcc gctggtaaat
    15361 gatgtaaagg nnnnnnnnnn gtaaaggtaa gcggaaatgc aacacagaat gcaaacgcat
    15421 ctgcacagac cgcacagccg cagcagtctc agccggttga cgacgatctg atggcaattt
    15481 tcaacatcct ggatcgcaag taagcgaaaa atcaaagaga aatacgaatc cccggtgcgg
    15541 ttttgcatcg gggatttttt gtacctgccc caggaaacac aaaaggcgaa ccgcagaagt
    15601 gcagttcgcc tttttcgatt ttatgtccgg gaatcacgca cagagctgtg tgcgctcccg
    15661 ttttttccag ctgataaagc cgtacatatc gttcatgaaa aagatcataa agttgatgat
    15721 gaccgggatg tagaccgggt cttttgccgc cgccagtccc cacaggacga tcagcaccag
    15781 atcgtttgcc gcatagccca gcgcatagta ggatgagcgc agcatggtga gggatgccgc
    15841 caggaaactg gtggtgacgg acagcgtgct gaatacgatg ttcggcgttt cgagccagcg
    15901 caggatgaag taaaatgcac ctgtcacaac gatgctcagc accagcagga acaggaagtg
    15961 ctttttctcc agcctgcgta tcgccacttc gcttttgctc ccggcggatg gattccgcag
    16021 ccaggtgact gccgaccaga ccgccatggg cagcgtcatg cccaggtaag ttatcatctc
    16081 accccaatag cgaaagcgga acgagatgat gccgtacagg atgctgaatg cgaccatcag
    16141 aaactgcgcc catacgttgc cctttgcggc aaagatcagc gaggtgatgc ctacccaggt
    16201 cgccgcaagg gtcagcacgt caaagctgcc gcccaccaga ttggagaccg tcacgatcag
    16261 catggagatg ctccacagcc accactcttt gacggataat tctttccatg gattctgcaa
    16321 ttggatatgt cctccttgta gttcgattct cgtttaccca tacagagaac agaccggatc
    16381 cagaatcggg atggttcttt ttttgaaaca cattttactt tgccgcagcc tcttgtcctt
    16441 cttccaccag ggcgaagtcc acgttgccgc tgttgacatc gatcttggta cactgtaccc
    16501 gaacagtgtc gcccaggcga tagctcttgc tgccctgttc ttccttgaat gcgatgctgt
    16561 cgtcgttgat ccagtcgctt tccggcaggg aggagacgtg taccagaccc tctaccgtgt
    16621 tgggcaggga gacatagaaa ccgaagtcgg tgacgccggc aacgatgccg tcgaagatct
    16681 cgcccaggtg ctgctgcata tattcagcct tgtagcagtc gtcgcagtca cgctcgatgg
    16741 tgatggcacg gacttcggtg tcgctggact gctgtgccgc ctggataacg aatgtgccga
    16801 aacgctcctg gatcgccgca gtgtccgggt tctcgcagta gctgctgaga atgcggtgga
    16861 tcgccaggtc gggatagcgg cggatcggag aagtgaagtg ggcataatcc ttcagcgcca
    16921 gaccaaagtg accgatggga tccggggcat aagctgcctt tgccatagag cgcagcacca
    16981 ttacgttcag taccggatac agctctgtgc cacgtgcgcc gtccaggatc tctgccaggt
    17041 gacggggctt gatgtcggtg aataccggaa cctctacgcc caggcgcagc aggatctgtt
    17101 ccagacgctc gatcttttcc ggagagggct tgtcgtggac acgatagacg aacnnnnnnn
    17161 nnnttgccac gttcccgtgc cacaacatcc acgcagacac cctcgtcatt gatgaccaga
    17221 gcgccctcag cagtttccag ttccggtgcg ccacggcgct tgcgttcccg gagacggata
    17281 tcgcacagct cgttcatcac cgggatcatg tgcttgacat ccttgtactt ttcccgcagc
    17341 tccggagagg ctgtgccggc aaggatctcg ttgacctcgc tgtaaacgcc cttgaccgca
    17401 gaacgaatga cagtcttgcg gaagtcatac ttcacgatct cgccgttttc gtccagctcc
    17461 atcagtgcgg agaatgccag acgatcctcg caggggttca gagagcagat gccgttggaa
    17521 aggctggtgg gcagcatggg aatgacctgg tttgcatagt agatgctggt gccacgctcc
    17581 agtgcctcat tgtccagggc agtcttggga cggacatagt gggaaacgtc tgcgatatgc
    17641 acgcccaggc gatagccggt ctcggtcttt tccagggaga cggcatcgtc caggtccttg
    17701 gagtgtgcgc tgtcgatggt gaagatcggg gtatcacgca ggtcaaggcg cttgtcgtat
    17761 tcttcctggg gaatgggtgc atccgccaac ttgtctgcct cggcgatgac ctcttccggg
    17821 aagtcgatgt ttacgccacg caccgccagc atagcgtccg cacagacgct ggcacgggtg
    17881 cagacaccaa agctgaatac gatctttacc cggtgttccg catggcggct gccacggtgt
    17941 actacctcag ccaggacttt ttcgccctcg tggtagatga cgctgtcctt gcgctgaatc
    18001 tggatgggcg tcttggacat ggtgtccgca cggaacagca gtctgccgtt gtcgtccacg
    18061 atcacgccgg tcagcttgct gtctgcctct tccaggatcg ccaggacttc gccctccggc
    18121 ttgccgctcc gggagggaat attgccaatg aggacacggt ccttggtcat tgcgccctgg
    18181 aggaactttc cggggatgaa cacatcctgt gcgccctctt cttcggggcg tgcaaagccg
    18241 aaggtacggc tcagacggac gatggtcgcc ggataatagc ccatgtttgc ggaagagaca
    18301 tagccacgcc gccgctcaca gatcgcaccc tctttgcaca gttcctggac tgccttccgg
    18361 aagttggctg cgccgccacg gttggtacgg cacttggtcg ccaggtcacg gtcgctcacc
    18421 ggggtgcgtc cgcttttttt cagaatggtc atgatttttt tgcggtagct gtcaattgtc
    18481 tcagcgccgc ccagttgggt atttgccata aatgataaat cctttcactt cagccgccca
    18541 ttgcggcatt ctcattgcgt catgtgtctg agaacctata cgcagacagg ttctgattcc
    18601 ggactgcttc gccgtatgct gcgaaaaggg aaaatgtcgc cggaaaaaag aaaaatcctg
    18661 cgcctcagtg ccgttccgga acggaaaaca cataaagcac aggacgagct gccattactt
    18721 cgtcagcagc ggcattacga tatccataaa caggatcagg acaaacaaaa taacggaaca
    18781 aacaaaagtg atcttcttca gacgtgcttc ctttgtgcgt ccacggtttt tgtcgtagaa
    18841 tgaatcattg cttgcgccgg tcagtgcagc agttgcattc tgcggcttct gttcctgcat
    18901 catgcagatc acgatcagta caatgctgat cagcagaacc acagcgccga tgatgatttc
    18961 cagcggtttc atggaatccc tcctctcgtg cgttttaggg gacacggaac agcgccgtgt
    19021 ctccggagcc agtccatgct gaacggggca gatacctgca tccgttcatt atcttttgta
    19081 ttataccaca ttcccgaaaa aaaagcaagc ctttttgcaa aaaaaacacg cagttcattt
    19141 ttgcggcagc accgcacctg cattttttcc cggtctgccg catacactgc accaaaaccg
    19201 cagagatcac aggaggaaaa tgctatgcaa aaaaaggaac tgcctgcacc caccgggcat
    19261 cacaatctga ttctggagga tcgttccaag ctgacgctga ccggcgtcac cgatgtggac
    19321 tgctttgacg agcgcatgat aaacctgttc accgggctgg gggagctgac catcaaggga
    19381 cggaatctcc acatcaatga cgtgagcgtg gagaccgggg acatgaccat caccggagac
    19441 atctggctgc tgcaatacgg cgaaaaggac cgccgtggac cgctgtctct tctgggaaag
    19501 ctgtttcgct gatggagctg gagacttttt tcaccgtccc ggagcagacc cggctgctgc
    19561 tgtatgcagt gctgctgggg atccctctgg ggatgggatt cgatgtgctg cggacgctgc
    19621 gcctgctgct gccccacgga aagcttgcca cagccctgga ggatgtgtcg tttctgctgg
    19681 tctgggcggc ggcactgctt tgtttttcca cggttctggc acggggcgag ttccggggct
    19741 actactggat gggcagcgcc ctgggctttc tgctgtaccg ctgtaccctg ggatgcgtca
    19801 ccgtgccgct gctgcgccgg atcttttcgg gtctgggcag gtttttccgg cggctgttcc
    19861 tgccgctgtt ccacggtgtt gtgcggattt gcaccccgtt gaaccggaaa tttggacatt
    19921 tcgccaaact ttttcgaaaa gcatcttttt tttcgcattt acccttgcag agagatagaa
    19981 aaatgttgta taatgatagt ggaattttga aacgaaaggc ggcgttatca tggcaaagga
    20041 aaaacaaacg gaaaaaaaga agtcacgctg ggtctttcgg atcatcagcc ggaccgcagt
    20101 gatcctggcg atcatcggat gtacggtcag tgtggtatcc atccagtcca gcatcgccga
    20161 gaaaaagacg gagctggaac aggtgcagaa acagatcgac aacctcaccg cagacaacga
    20221 ggatctggaa cggatcctgg agagcggcga cattgacggt tatatggagc gcctggcacg
    20281 ggaagaatac aactacgcat atccggatga attccggttt tatgacacat ccaggaacta
    20341 atcagatcat tttcgggcag cagtacgcaa tgggaacaga aagggataag gagttatatg
    20401 caggtcgaga tcggaaaaat ttatgaggga acagtcaaga atatcaccaa gtacggggct
    20461 tttgtggaga tcccgaaccc ggacggcgat aagatcatgg gaatggtgca tatctcggaa
    20521 gtttcccgga gctatgtcaa cgagatccgg gactttgtga cagagggaca gcaggtgcgt
    20581 gtcaaagcca tcggcacaaa tccccagggc aaaatcagcc tttccatgaa acaggcagaa
    20641 gagggcgcag aacagcacgg acagcgccgt ggcggaaacc acggagagcg cagcggcaga
    20701 ccgtcccgtg gaaacggcga tgcaccccac ggacagcagt cccgtccgcc acgccgcagc
    20761 aagcccaatg tctgggagcc gaaaaagcgg caggaaccct cagagatgac cttcgaggat
    20821 atgctctccc ggttcaagca gaacagcgaa gagcggatgt gcgacatcaa gcgcaacacc
    20881 gagcgcaaaa accgttccaa gcggaaataa ggatctgtat tcatatagcg tatgcggtgc
    20941 ataccggttt tcagagattc gggcagtcct tcggggctgc cttttttgcg tgacggaggg
    21001 cgttcgcaga atctgaaaaa actgtacata ttgccagaaa agatatcgga aaaactaggg
    21061 gaaatgccag ttttccgggt gccggggagt tttctgggga tagatattga catttcggcg
    21121 gttttccgat ataataagga aagtgcagcg agtgccggaa aatccggcgc agactgcatt
    21181 tcctgagcaa tagcaaactg aataatttca cggattgata ggggaatgcg tattgaagcc
    21241 ggtacctttt gagcaactac cggatgagga gcttgtgctt ctggcgagga acgaccgtgg
    21301 ggcgatggag acgctggtga aacgctatac cgatgcggtt tggcatcagg tacggcagtt
    21361 tcggggcgct tctgaaccgg acgatctcgc acaggagggc tttttgggtc tgatttctgc
    21421 ggtatgccgg tatgattcca tgcgtggcgt acctttttct gcctatgcgg gcaaatgcat
    21481 tacaaacagc attgtatctg cgttgcggcg ctgccgcaat ttaccgttac ctgtggggac
    21541 atatgatgtg cctccgcttt ctcagatgca ggacaaagcc ctgccgccgg attctgtggt
    21601 gcagagccgg gcgcaggcgg cggcgatgtt ctgcgcaatg gtgaaccgct tatcccggcg
    21661 ggaatatcaa gtttgcatac tgatctacgg cggtgcctct tatgcacaga ctgcacagca
    21721 gctcggcatc tccgtcaaat cagtggataa tgcgctgcag cgtgccagaa gaaagcttca
    21781 tgcagaaggc atcgtgcagt gattgcaccg ggaccctccg gacggcaggc tttttgccgc
    21841 cggaacattt tttcaattga acagccggac gaccgctccg gcacgaggat tcctgagcgg
    21901 attccctcga gaaaggtaag gttatactat gtacgaagat aagactttgg tatgcaagga
    21961 ctgcggaaat gaattcgttt tcactgccgg tgagcaggaa ttctatgccg agagaggttt
    22021 caccaatgag ccgaagcgct gcaaggcttg ccgtgatgca aagcgtggcg gcgcaccgag
    22081 acaggaacgt gagatgttca ctgctgtctg cgattcctgc ggcggcgagg caagagttcc
    22141 gttccagccc cgtgaaggcc gtccgatcta ctgcagcgct tgctatgcaa agatgaagga
    22201 agaggcataa ctcttaccgg actgtgatag accggcgttt tcgccggaaa gattcttttt
    22261 gtctgcatag ccgtttccgg aaagggaacg ctattgattg cagagatgca gcgtttttgc
    22321 tgcacgatgc acagacagga ggtgttagga atatggcaac aattacaaag gacaccatta
    22381 tcggtgatat tctggacatt gcgccgcaga cagcaccgct gtttatggcg atcggaatgc
    22441 actgcctggg ctgcccggct tctcgtggag agacagtcga agaggcctgc atggtgcatg
    22501 gtattgatac ggacgaactg ctggaagaac tgaacaagca gatcgctgcc ggcgatgcac
    22561 agtaagagcc tgtttcgtgc ttttccgtgt ctgctttttc ggacaccgga cagactctaa
    22621 aaaaggatgg aattgctccc cgtgtgaatc ctgccgccct ggtggtagtt cccggcggag
    22681 caattttgtt tttcagcgaa aggaacgatg cgatcatgtt gacgaaacgt cttgccgcct
    22741 gtgccgcact ggtgacgccg ggcggcgttg tatgcgatgt cggcacagac cacgcctatc
    22801 tcccggcgga actgctgcgc cggggacaat gcacccgtgc tgtcgccacg gacatccacg
    22861 ccggacctct ggaggcggca aagcgcacgc tgacagaagc cggggtgctg gaacaggcag
    22921 agctgttcct ctgtgacggt ctggcacagg tgcagccgga gggcatcacc gatgtggtga
    22981 tcgccggcat gggcggtgag acgatcatcc acatcctgga ggactgcccc tggcggcggc
    23041 aggtgcatct gatcttgcag cccatgacga agattcccga tctccggcgc tggctggcag
    23101 agcagggctt ttcctggacg gaacaggcag tgcaggaagg ggaaaagttc tatgtggtgc
    23161 tgagcgtttt ccacagcggc acacccggca tcctgtctct gctggaacag gaagtaggct
    23221 gtctggattg gcagtctcag accgcacgcc gttatgccgg ctggaaacag cagcgttttc
    23281 agaaactggc acagcagctg cggcaggcac agcgcccgga ggcggagcag tgggagacgc
    23341 tggcggaaac catacagaaa aaattgcagg aaacggacgg gaaaggagaa acatcatgct
    23401 gacggtaaaa gaggtctatg aggcggtcaa cgggtttgcg ccctttgcat cgcaggagtc
    23461 ctgggataac tccggactgc tggtggggag catgacccag ccggcagtcc gcattctcac
    23521 cacgctggat atttcggaag aggtgatcgc acaggcggtt cagcagcagg tgcagcttat
    23581 tgtggcgcac catccggtga ttttctcgcc gctgaaagcg ctgccgcaga acagcccggt
    23641 ctatcagctg gctgcacacg gcatcgccgc catctgtgtg catacgccgc tggatatgtc
    23701 tcctgtgggg ctgaatgcct atgcccacgg actgctggca gaaccgctgg cgctggaaaa
    23761 cgcacagagc gtcctggaac cgtcctggac ggacggcaga gggttcggct ggatcgacac
    23821 cagcacccgg acatggacgc cgcaggcgct ggcacaggtg ctggaaaaga cgttcggtat
    23881 ggtgcgatat tccgccaggg aaacgcccat ccggaagatc gcctattgtt ccggttcggc
    23941 ggcgtcgatg ctggaactgg cggcggcaaa gggctgcgat gcgcttatca ccggagacat
    24001 caagcacgac cggtggtatg cggcagagtt cgaaaacatc gccctgtttg actgcggtca
    24061 ctacggcaca gagcgctttg cggcagagat cctggcgcag cagctccggg acgcattccc
    24121 ggcggcacag gtgactgcca atgccggaga cgatccggca aagattttca taggagatga
    24181 aaaagcatga gtgtttggaa atgcccggtg tgcggttcgt tcatgcagca tcggggaaga
    24241 gcctggagct gttcccaggg acatagtttc gatatggcga aaagcggcta tgtgaacctg
    24301 ctgccggcaa accgcaagca cgccaagaat ccgggagaca atgcggtcat gatgcgtgcc
    24361 agaaagcgtt ttctggatgc cggtcactat gcgccgctgc tccaggcgct ggaacaggcg
    24421 gcagtgcgct atggcagagc cggcggcgtt ctgctggatg ccggatgcgg cgagggatat
    24481 tataccgagg gtgtttccgg ggcgctcgcc aaggcagggc tgcccatgga gacctatggg
    24541 gtggatattt ccaagagtgc agcagatgcg gcggcaaagc gctgcccgca tgcccatttt
    24601 gccgtgggca gcgtgtacca tctgccggtg gcggacaagt gctgtgatat gctcatgacc
    24661 atctttgcgc cctactgcgg tgaggagtac catcgggtgc tgcacaaagg cggcactatg
    24721 atgatggtga ttccggggca gcagcatttg tggggactga aagaggcgat ctatgatgtg
    24781 ccgtatccca atgctgtccg ggactatgcc ctggacggat tcgaactgat ccaggcggag
    24841 tatgtggaca gctggctcaa gctggacaac ccggcggata tccagaacct gttccaaatg
    24901 acaccgtatt attacaagac cggacgggaa gaacacgctc ggctggaaca gctagaaagc
    24961 ctgtccacgc agatctcttt tgaaattttg cagtatcggg cagtggcaga aaccggcgaa
    25021 aagtgagtgt tccgggagga atttcatgaa gaatatacca gaacgggtgg aaaaagagat
    25081 cattcagctg gcgaaacagt atgatattca gaaagtgatt ctgtttggct cccgtgccag
    25141 aggtgacaac tgggagcgga gcgacattga cctggcagtt tccggcggtg acattccccg
    25201 gtttgccttg gatctggatg aagaggtctg gacgctgctg ctgtttgacg tggtgaatct
    25261 ggatgaaccg gtgcagccgg cactgctgaa agagatcgca cgggacggag ttttgattta
    25321 tgaaaaagta tgagaatttt tgcagtatcg ggcagtggca gcggagtaac tggatgtaat
    25381 agagtgcgag tatggttttt cgcacggggc gatgtgggca tcgcccccta ctctctcata
    25441 gatttttacc aacaaaaatc ccccggcggg gcgaacaggc ggctgctgcc gccgtaacac
    25501 tgcccttttc acaattgaaa ttggggcgta aggtcagtgg cgacccgcca caggaaagga
    25561 tgaaaccaac atggcattag acggtgcata tttacacgca gtgcggcagg aacttctgcc
    25621 gctgatcgac gcacgcattg aaaaaattca ccagcccaca cgggaagaac tgatcgttgc
    25681 ctttcgcagc cgcaccggcg gtgcaaaggt gctgttctcc tgtgcggcag accgggcgag
    25741 agttcacctc acccaggtcg ctgtcgaaaa tccgaaagcg ccgcccatgt tctgtatgct
    25801 cctgcggaaa cggcttggaa acggcaagct cctggatatc cggcaggacg ggctggaacg
    25861 tgtgctgtat ttcgacttct cctgtgtcaa cgagctgggg gatattgtgc agctgaccct
    25921 tgccatggag atcatggggc gctattccaa cctcattctc atcgatgaga ccggaaaggt
    25981 cgtcgacagc atgaagcgag tggacgccac catgtccagc aagcgtctgg tgctgccggg
    26041 gctggcatac gaaatgccgc cgagagacga acggctcaac tttttgcagg cgtccccgga
    26101 gcagatgctg gcggtgcttt ccacgaaaac gggagaactt tccaaggcgc tgctgcaaac
    26161 tttcgagggc gtttcgccgg ttctggtgcg ggagtgggca tactataccg gaaagggaca
    26221 gccctgccgg gcggaatccc ttacagacga ccagaaagac cggctctgct ataccatcgg
    26281 gcgtacccgg gagatgctgg aacagggcga cacggtctat accatcgtca gcacccgtga
    26341 ggggcagccc aaggactttt cgttcctgcc gctgcatcag tacggcacgc tgatggtgac
    26401 gaaggtcatg ccctctgcct gtgcgctgct ggacgaattt tacgccagcc gggatcacat
    26461 ggcacggctg aaacagcgtg cgaacgacct gttccacctg ctgctccacg ccaccgagcg
    26521 tatccagcgg cgtatcgtct cccagtcgga ggaactggac gcctgtgacg caaaggacga
    26581 tgaccgtcgg aaagccgatc tgatctcggc gaacctgtat cgtctccaca agggcgacac
    26641 agaggctgtc ctggaggact tctacgagcc ggactgcccc acgatacaca ttgccctgga
    26701 tgtgcgtctc acgccgccgc agaacgccca gaagtactac caggcatata agaaagcctg
    26761 cactgccgaa aagatcctga cggaacagat cgccaagggc agggaagagc tggcgtatct
    26821 ggacagcgtg ctggatgcgc tgagccgtgc cgagtcggaa aatgacctgg aacagctccg
    26881 ggcagaactg gcggaacagg gctatctccg gctgctgcgg cgcaagggca agccgccgaa
    26941 gtgcctgccg cccatggaat ttaccgcatc cgacggcaca cgcatcctgg tgggacggaa
    27001 caaccgccag aatgaccagc tgacgctgaa aacggcaaac aaaaacgaca tctggctgca
    27061 cacccagaac atccctggct ctcatgtgat tctggagacc ggcggcaaag aaccgtccca
    27121 gaaagtgctg gaagaggcgg cacagctggc ggcataccac agcaaggcac gggattcggc
    27181 acaggtgccg gtggactact gcgctgtgcg gtatgtgaaa aagcctgccg gggcaaagcc
    27241 gggcatggtg atcttttcca accagcatac gctgtatgta aagccggaac agagatgcca
    27301 gtcggaggga taaaagatga gacaagtttg tccgcactgc ggtgcgccga tgtatcataa
    27361 ggtatgcgaa tactgcggaa tgccggcaaa acagccgaca gcgtctgccg ggacaaagag
    27421 ccggaagttc gtctggctgg cgatcgcact ggcggctgct gtgcttttgg gcgtggtggg
    27481 agtgacatct gtggcagtga aacgcattca ttccggtgtg tccacggtcc ttgccggcaa
    27541 gtctgaaaac agcacaaatg acgcagcggc tctgaagaaa atgcaggaca gcggcgtgtt
    27601 cccttcgggg acttatctgg ttggcgagga gattccggca ggggaataca tcctggtttc
    27661 ggagtcctca gaaacagatc atctgttcta tgccggcatc tatgccgacc gggaaaaaga
    27721 gcaggagatc agcggaggct ggaacgagtt ttccgtcatt gtgcagctgg aagaaggcac
    27781 atatttcagc ttttcctggg cgaactgcta tgatgtgacg aagaagaatg tccccaacga
    27841 cccgacgaaa catccgggaa tgttctgcgt gggacgggac atcgaccccg gaacctacac
    27901 cattgtgccg tatccggatg aaccgcacga actgcatccg acctatgcgg tttatacaga
    27961 actgggcagc gttgcaccca tgatcgcaga gtccgggagt ctgtacaatc cggcaaacag
    28021 caaggtgctg gatagtgctg aggtgacgct ggaagaggga cagtatctga acctgtcgga
    28081 atgtatgatc gcagagtaaa aaaggaggta tcccatggca ctcactcgtt tgacggagtc
    28141 cttttatcac cgtgactgtc tggaggttgc gcccgatctg gtgggcaagc tgattttgcg
    28201 aaagttcccg gacggcacac tgctccggga acgcattgcg gagacagagg cgtaccgtgg
    28261 gcaggaagac ctggcgtgcc atgcctccag gggcagaacg ccccggacgg aactgctcta
    28321 ccgggaaagc ggcgtgatct acgtctatct ttgctatggg atgcactggc tcatgaacgt
    28381 catcaccggc gagccggaac agccccaggg cgtgctgatc cgtgccggcg cagtccacaa
    28441 cggtccggca aagctgacca agtatttgca ggtggacaag cactttaacg gcggcagttt
    28501 cgtcaccagc ccggaactgt ggatcgccga tgacggctgc cgcccgaaac tgcgcaccga
    28561 tgtgcgtgtg ggcatcgact acgccgggga gtactggaaa aatatgccct ggcggtggat
    28621 cgctgaggaa atataaaaag aaagtcacct ttggtggaag aggcggcttt ctgagaaaag
    28681 gtgcataaaa aatcgtgtta tcccttgaca cgatcatgga attctggtat aataaaataa
    28741 agaaaagagc atacaaaaac agtatgctcc catattgaga tgttaagaga taaccgccca
//
LOCUS       DAWENO010000006        26598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_751_length_26598_cov_12.154881, whole genome shotgun sequence.
ACCESSION   DAWENO010000006 DAWENO010000000
VERSION     DAWENO010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 26598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26598
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_751_length_26598_cov_12.154881"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5812..5821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25758..25767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5746 a         7632 c         6634 g         6566 t
ORIGIN      
        1 gacctacgat cttgctgttg agtgcgccgt tgatgagact ggtcttacgc accttttcag
       61 tctcatcggg cgtgaggaaa tagcagccgc acttcaaaaa ttcctggcgg actgcgtcat
      121 aaatggtttt gtcaacgatc acagactgct ccgatgcgca gatcatgccg ttgtcaaagg
      181 tcttagagtg tacgatcgag tttaccgcct tgcggatatc cgcagaggca tcgatcactg
      241 ccggggtgtt gccggcacca acgcccagcg ccggtttgcc gctggaatat gctgcacgca
      301 ccattcccgg accgccggtt gccagaatga tgtcagcctc ctgcatcagc gtgttggtca
      361 tttccagaga cggaatgtcg atccagccga tgatattctc cggtgcacct gccttcacgg
      421 ctgcatccag cacgatcttc gccgcagcga tggtgctttt ctttgcacgg ggatggggcg
      481 aaatgataat ggcatttctg gttttcaggc agatcagcga cttaaagatc gccgttgacg
      541 tggggttggt ggtcggaatg accgcagcca ctacgccgat cggttctgca atacgggtcg
      601 tgccataggc gggatcctgt tcgatgatgc cacaggtctt ggtatgctta taggcgttgt
      661 agatatactc agcggcatag ttgttcttga ttaccttgtc ttctacgaca cccattccgg
      721 tttcttccac tgccattttt gccagcggca gacgtgcgtg gtttgcagcc agcgcagctg
      781 cccggaagat ccggtctacc tgttcctgcg agaatgtcgc aaactgtgcc tgtgcctcac
      841 gcacttcctt cagcttgcgt tccagcgccg gaacgccatc caccaacggt cggttgttct
      901 ctgtttgatt cattcaaata ctcctttttt gggaaacgcc gaaacagtcc ggtgtcatcc
      961 gggtagaaga agtcccggat cttctttttt ccaaaagcgt gagagatttg tgggaaaata
     1021 cagcgaaaag gcatcagaac ccgtttcctg ttccggcaga tgcgccgaca ggcacgaaac
     1081 gagcgctgac gtattttcgc agcgattgac tgtatccagt cgtttccgtt gaatcatgga
     1141 cggtttttct tctgtgacaa cgatgtccat agatccccat ctgtgacacc tttgtcaatg
     1201 ctattatacc acaattccac tgctttcgtc aagtcataaa aatttcgcac acaaacgcca
     1261 tccatacggc atcacccacc gaattccccc gtccgttccc gacaggtatc gcaccgcaga
     1321 agtggtatac tggttctcag aggtgatgac agttgcccac gatctacctg gacgttctgc
     1381 tgttgcagag cctctatgtt aactattttc tgctgcgtgc ggctgcaaag ctgacgcata
     1441 cgcctttggg atggctgcgg tgcggactgg ctgccgccgg gagcagtctg ttttctctgc
     1501 tgatcctgct gccgccttta ccggtcctgc ttcagctttt gctgaaactt ctggcagcag
     1561 ctgcggcggt agtcctggcg ttcggactgc accgggaaag ctttttccgg cagtgtggct
     1621 gcttttttct ctgcaacttc ctgcttgccg gactgatcct ggcggtggac agtctgacgc
     1681 cgagaggatt tgccgcctgg ggaaacagct gctgttatct caatttttcc ctgttccagc
     1741 tggttctatt cactgccgcc gcatattctc tgctccatat ctgcaccctg ctccgccgcc
     1801 ggcaccggca cacggacgac cgctttcagg tctttttccg gctgggcagc cgccagctgg
     1861 tactggacgg actggcagac accggcaaca atctgacgga caccttttcc ggcacaccgg
     1921 tcattgtatg cagtgccgca gcccttgcgc cgctcctgga cggcacgccg ccggaacagc
     1981 tgaaaggcta ccggctgatc ccctgcgcca ccataacggc ccagggactg ctgccgctgt
     2041 ttcgtccgga tgaggtgtgc atccggaact gtaccaccgg aaaaagccgc cgggtggatg
     2101 ccatggtcgg catcggcgga acgcaacaga acgccatctt tcacccacaa ctactcgaat
     2161 gaaaggagtc cttttcatgc aacccattct ccggaaattc cgcctgctgc tctcccagct
     2221 gcggggaaaa ttttctccgc tctgccgggt ggactacatc aatggttcgg atacctttcc
     2281 gccgccgctc tccaaagagg aggaacaggc ggtttttgcc cggctgcacc agggcgacac
     2341 cgccgcacgg gagaccctta tcgtccacaa tctgcgcctg gtggtctaca ttgccaaaaa
     2401 gttcgaatcc tccgggctgg ggctggagga tctcatctcc atcggcacca tcggtctcat
     2461 caaggcagtg gagaccttct gtccggacaa gaagatcaag ctggcgacat acgcctccag
     2521 gtgcatcgaa aacgaaatcc ttatggtgct gcggaaaaat gcccagctcc gtggcgagat
     2581 ctccatcgac gagccgcttt cttccgactg ggacggcaac gagctgctgc tctccgacat
     2641 cctggggaca gacgaggaca tcgtaagccg ggacatcgag aacgaggcgg aacgggagat
     2701 gctccttgcc gccgtctccg agctgggcga acgggaacag gagatcatgc agatgcggtt
     2761 cggactctgc ggcaaaaaag aacgcactca aaaggaagtc gccgaggcga tcggcatttc
     2821 gcagtcctac atctcccggc tggaaaaaaa gatcatccgc aaactgcgaa aaaagctgga
     2881 agaggcagac cagcccagtg tccacactac ctgaaacacg caaaagagcc gcagggcatc
     2941 acgctctgcg gctctctttt ttatgatggg gttttcatgt tgtcaaaaca gtttgaatta
     3001 ttcctcgctc ggcagggact tgatgagtgc taccaagaac ttcagcagaa caactgcatc
     3061 gttcgaaccc agttcaccat tgccatcaca gtcggagttt gcctgctgct gtgcgctttc
     3121 cagtgttaca gcgccggcaa ctgccttgtt cagcagaact gcgtctgtga tatccacacg
     3181 gccgtcacag tttacatcac cgtacagcgg gatgatcttt tctgtggttg tcgtaccggt
     3241 tgtgctgctt gtgcttgcag ttgttgcggt agtcttagaa gtagcagtgg tagtagtggt
     3301 ctttgcggtt gtggtagtcg tctgagaagt ggttgtcgtg gtagtcacgg tcggagtagt
     3361 ctcagttgcc ttcggcagga cattgactgt cacttccttg gtctgaccgc caggtgtggt
     3421 aaccttcacg gttgtagtac caacattttt accgctgata accaggatct tgtcgttgtc
     3481 cggatcagca gcaacgccaa ccttggtctt gtcgtccggt gtaccggtgc agttcggaac
     3541 gtttacttcc aggtttacct tttcgcccag tacaacatcg atggaatcaa caacttccag
     3601 ggtatcttcg aacatatcct gcttcttctc attgttctga tcgtctgccg catagtaaac
     3661 ggttacgttg ttaatggtgt acttggtgga ctttgtttcg cccagacctg cataccaaac
     3721 gccgaagctc atgtgtgcat cttcggattc tgtgtcaacc tggttccatg cgttcagtgt
     3781 gttgccggtg ctttctgtgc ggatgccctc tgccagtgtc ggcggaacga tccacataaa
     3841 gctgtaggtc tttgtgcctt ctgcatcctt gtacggtgta tcttccagag aatcatctgt
     3901 ggaatccgga cggttgcctg cggtactctt ttcccagtac agcagtgtag gtgttgtggt
     3961 gttcaaacct tcgttcttaa acatatcatc agactggaac cagttatcag tgaggtcagc
     4021 cttttccaga gtggaagagc ctacgccaaa ctgtacctga tttgcgtcct ggtctgtggt
     4081 tacatcaaaa gcgactgcat aaacatcagc agtcttgtca tacttcatgc caaagtcagc
     4141 atacgggatc tcggttgcat cgcccacgct gttggaacct gcattcttga tggaagcagt
     4201 gccgctgcac gggaatgtga cggattcttc ataagtcagc tcagctgcaa cgatgctgca
     4261 gtctgtgaac tttgcaccct tcagtgctgc ataccacagc tggaaggaga tctggctctt
     4321 ttctgccaga gtttcgcagt caacgacctg ttccggaacg tcccaggtta cggtgaagta
     4381 gctgcccagt tcctgctttg ccaggtctgt gccgccgccg acggtgaagt cacccagtcc
     4441 gttgccccac tttacgccag cgtctgtaca ctgcttcaga gattccggac caatgtcatt
     4501 gtaccaatag ccgactgtct ttgcaacgcc gccttcgccg ctgctgccct tcaaaccagc
     4561 ctgttcctta gcatactcag tatctgccag gctcttataa gccacgttca gaccgccgcc
     4621 gtacataaag cggtttacat ttacatcttc acctttttcg ccgttcacct taatggtaac
     4681 gcttagggac tttacctttg catcatccgc aacggtagca cccgggcgga agttgaattc
     4741 accataggtg aacttgtggc tgttcagcgg cagacctgcc tcacggatcg ctgcctccga
     4801 ttccagagcc acgccgtcgt tcttttcctt gaatgcgtca gatgcgtaat actcttcact
     4861 gcagttgtca ccgggtgtca gtttgatatc caggtcattc agcttcaggg agttgatggc
     4921 ttttacagtt acgttatcgc cgttctcctg cacgtcagaa actttgccgt ttccgatgat
     4981 gctggaatag ttcagaccgc cggtgttttc tgcacccttc ggcagagtcg ggtctacttc
     5041 atagatccag tctggctttt cgccgttgct ggtatcggtc gtgccgttta ctgtgataga
     5101 caccagttcg atctcagcat cagtggtgcg tgtggtgtga tcctcttcac tgataagacc
     5161 ggcatagcag ttctggaact ggaactttgc atccttattg ctggacagtt ccttggctac
     5221 gctggacggc agttctacaa caacagagaa ctccttggca tatggtttgc acttgattgc
     5281 acctttggaa tcggtttctt caaagcccca ccagtagccg ttcgccttag tatctgttgc
     5341 gccgaagtag taagagaaat ctgtgaccat ctggtcagac ttcagattaa actgcaaaga
     5401 ggtaatactg tcaccgtaaa tatccgccgg cgacttaccc tctgtgtcag taaatgaagt
     5461 ataatcatct gtcttggatt tgcacagata ggtacactta tctgcattca gcggattgtc
     5521 cttatcgtcc ggattcggga tagaagttgc cagcgtcttt ccaatgttca cagtctcgcc
     5581 ctcagccgca tttgcaacca taaagctgcc ggatgcaacc gcaacggaac tggtcgccat
     5641 taccgcactc atcagaagtg cgcttactct tttgcgaatc actttgctca tgtatatcgc
     5701 tcctctttct ttcaattgtt ttttcatctg ttccaatctt ttctttctgc atccacaaca
     5761 gcaactgtca tgaatattta tgcttaaaaa atggtacatt cattttttag cnnnnnnnnn
     5821 naaataaaaa aataaaggac aatctcatca agaaactgtc ctttatccaa atatttacac
     5881 tgttaattat ttccagttgt tgtcaccgtc gacggattct gacctgctcc ggcagttgtc
     5941 gtggctgcac tgcgcacgcc ctgcacggca acacggtaat cgcccaccgc ccagacggtg
     6001 ttatacttgc tgcacgagat cgtgggcgtg tagttgaatg tgatggtatc gctgtccact
     6061 gcgcctgcct gggtggaata gctgagcaga tccacatttg cggtcaggtc gctggcatcc
     6121 agttcatcca tcacgtctgc cggtccgatg atgctgactt tcagctgttg tgtcagcacg
     6181 ttgaaatcat acgttcccgg cgcattgacc acgccgatat ccgagatcac aaagtccttt
     6241 ttcaccaggt cggcggaatc cagagacaat gtcacggtag agaatcccga ctggttcaca
     6301 tagtcatccg gcacattgac cacaaagtcg ttggaataat ccaggtcgat atcccgcagt
     6361 gccacagagc cgatgttgaa ctcgctcatc tcctccatcg aggtgttggg cgcagccagg
     6421 gtgatctgct gttctgacag atgcagccgc tgtttcaatg cgtccaggtc gaagttcgcc
     6481 ggggcgttgg tgatctgata ggtcacatcc atggttttct ggtacaatac cggcacttcc
     6541 agagagaacg ccgccacatc ccaggtgaag tccttggaat ccacctgcgt ccctgcctcg
     6601 ttatagaaga ggagcgtatc cgtggtgtag atcttggagg cgctgagctg ttctttctgc
     6661 tgggtctctg ccacgcagta tttcacctga tccagctgtt tctgcggccc ggtgacatca
     6721 atggtagacg gcgtgcaggc gaagttctct tcgtccagga cacagccgtc cgtaaacgtc
     6781 agattcggcg cagagggctt gatttcaaag gtataggtgt cgatcttatc aaaggtcaca
     6841 ttcaccgtag agggcgaaat ggacttcacg ttgaactgtt catttccgtt tccggtgaca
     6901 gtaatggaaa gggtctttgt gccggcagag gtcacgtttt ccaccacagc ctctgcggtc
     6961 agattgtccg cctcaatgtt gccgactttc gagcgcttgc cctcgatctg caccgtcaca
     7021 tcttttatgt catagtcgat gacgctgagt ccgttttctt cggcagacgt tccggcgatc
     7081 tccactttca gcggcacatg gtgcagcgtc ctgggcgtcg tggggtaaat ggcaatggaa
     7141 atgacaaacc aaaggacaat ggcaatcgcc acagaagtaa tcatcatggc aggacggctg
     7201 tcatagatcc gctgaaacag cgcccggtca cttccggact tccggttctt ctgtgatgtc
     7261 tttttctgtc ggttcttcca cagattcttg ggcatacgcc ttctcctttc cgtcgtcctt
     7321 gtgatgccgt ttgaggaacg ggatcttctt tgccactctg ccgatggtcg tctcttcgcc
     7381 cgccgtaatc agacgtgctg tcaaaagctg gtgcagagac tctctggtaa agccgctttt
     7441 cagcacgccg ttttccgcca cagagatcgc cccggtttcc tcagaaacca cgatcaccac
     7501 ggcatcggag ttttcgctca tgccgatcgc cgcacggtgg cgtgagccga gacgcttgtt
     7561 gatgagttct tccttctggg gtttcggcag aaagcaagcc gccgccagga tcttgccgtc
     7621 ccggataatc accgcaccgt cgtggagcgg tgtcttgttg aaaaagatgt tcccgaacaa
     7681 ttccttgctg gggatggcgt ccagtatcgt gccgttgtcg atctgttctc ccagccgtgt
     7741 ctgccgctca atgacgatca gcgcaccggt cgatgtggcg gaaagctcca cgcaggcatc
     7801 gcaaatcaca tcaatggtcc tgctccagac cgagctgtct acattggagc tgctgctgaa
     7861 ccgcagtccc agcttggtgt accctgcctt ttccagagtc cggcgcagtt ccggctggaa
     7921 gaggatgata agagcgatca gtccgatgtt cagcgtttca gaaagcagat aggaaatcgc
     7981 cttcatattg atccaggcag agatgatgta aaccagaatc agcaggaaca cgcctttgag
     8041 cagctgtccg gcacgagtct cccgcaccag cttcatgccc agatatacca cataggacag
     8101 caccaggata tccaggatat ccaccggttc tgctgttttg ataacgctga agatcatatc
     8161 caggatatcc cgtaaagcca tgcgcatcat ccttcctttc cgacttctct gatatgtacg
     8221 ccggacagag catcacgctt ttctgtccgg cgaaggttct attctccggt tctgtctgca
     8281 ttcccgtcgc tgagaatgcc ctatattact attgtaacat atcaccggaa tttttcaata
     8341 ggttttttgg acttttcaga atattgacat aatttttcag atttgccccc aggcgcaggg
     8401 tatcggatgc ccggctgaac gccgacggcg caaaccggac agtgccgtcc tttgtcccca
     8461 gcttttcgtg cgccagcggt gcgcagtgca gtccggcacg caggcaaaag ccctgtttgt
     8521 caagatatgc ggcagtctcc tccggcgatg caccatccac cgtaaaagag acgatcggca
     8581 cgtatgccac gcccggcttg cggtacacag tgatctccgg cacatccgcc aacgagtcgc
     8641 acagcagctc acagagacgg ctttcgtgcc ggtacagtcc tgccaccgtc tgccgccgga
     8701 caaagtcgat cccggcacag agcgacgccg cacctaccac attcagcgtg ccgcattcca
     8761 gccggtcggg cagaaagtcc ggctgttccg gactccgtga cgcactgccc gttccgccct
     8821 gcatcagcgg ctggatggga aaggcacagt ccgtcatgag gattccggtg ccggtgctgc
     8881 catataagcc cttatgccct gcggtgcaga gaatgtgcat cccgtcccgc tccagggaca
     8941 agggcaggat accgcacgcc tgtgcgccgt cagcgatcat gcagacgccg tggctgcggc
     9001 acacctgtgc aatctcccga tagggcaaaa tctgccccgt cacattgctg gcaatggtgc
     9061 agaccaccgc cttggtgttg ggacggaaca gtccctgcaa acgccggatg gtctcgctgt
     9121 cgttctcgga gacctccagc aggctgtacg tcacctttcc ggcacggaca agcgcagcga
     9181 tagggcgata gaccgagtta tgctccaggt cgctgaggat cacatggtca cccggtttca
     9241 gcacaccctg aatcgccaca ttcagcgcat gggtgcagtt taaggtaaag atggtatttt
     9301 ccaccttggc atcaaaaaaa tccgccaccg tctcacgggc atggtacacc atttcggagg
     9361 tgcgctccgc aatgacgtgt ccgccacgtc cggcgttgcc gtaccgtgca atggcatccg
     9421 ctacggcacg gcgcacctcc ggcggcttgg gataggtggt tgccgcattg tcaaagttca
     9481 ccgtcaccgc atatcacgtc cttccccggg gactccgaaa cggaattccc tctctggaca
     9541 gaagtgcgga gacacggccg cagtctgctc ttgccgccag aacaaaaccg cagccctctg
     9601 gccccaggtg ttccagccgg cgtatctcac tggagatgcc atactgctgc agcgcctgtc
     9661 gtgcccggtt cgcctgggta taggagacca ttgccgcttt acattccaac atcttctcac
     9721 ccctttgcat attgatcggg gaaacacttc cccgtgtcag tatatgcaga acggcggcga
     9781 tgcggtgaca ggagttatac agtgtttcct ttatgccttt accagcaggc agacgcagtg
     9841 cgccgagatg cccagtccct ctccggtaaa gcccagcttt tcctctgtgg tggctttcac
     9901 gctgacctgt tccacagaaa caccgcaggc tgctgcgatc ttttcccgca tgggaacgat
     9961 atacggtgca agctttggtt tctggcagag gatcgtggca tccagattgc ccacggcata
    10021 gccctgtgcc tttacctttt ccaccaccag ttccagcagc tttaagctgt ctgctccggc
    10081 ataggcaggg tctgtgtccg gaaagtgctt gccgatgtcc cccagcgcca gcgcacccag
    10141 cagtgcgtcc gaaaccgcat ggagcagtac atctgcgtcg gaatgcccca gcagtccctt
    10201 ttcataggga atctctacgc cgcccataat cagctttctg ccctccacca gacggtgaac
    10261 gtcatagccg tgtccaatgc gaaacgggat gttttccatt tatgcctcct ccttttggcg
    10321 agccagcagg atctccgcaa tggcgatatc ctccggcgtg gtgatcttga tgttagtata
    10381 gtccccctcg gtcatgtaga ccttacagtt gatcgcctcg atcagctggc agtcatcggt
    10441 gaaatccagg gcgtgttcct ccgcaaaggt cactgcctca aagtacagct gccgcttgaa
    10501 cacctggggt gtctgggtga taaacaggtg aggacgatag ggcgtgtcca cgatcagatg
    10561 gtcattgacc accttaatgg tatcctttac cggcacaccc agagtcgcac cgccgaaaac
    10621 cgatgcgtcc cggatgacct gtgcaatgtg ttccggtttt accagcggcc gtgcgccgtc
    10681 gtggacggcg atcattgttg tgcctttgga gagcgcacgg agaccgcaca tcacgctttc
    10741 ctgccgggtc gaaccgccgg caacgatggc gtggaatttt gtgatgccgc actgctgtgc
    10801 cgtttcccgg atggcatcgt gatcggacgg acgtgccacc acgatgattt ccgccacacg
    10861 tgggcaagcc tcgaaggcac gcatactcat gccgatcaca ttggtatctc ccagcggcag
    10921 caggatctta ttctttcctt gcatccgggt ggcgtttccg gcgcacacca ggatcacaga
    10981 ggtgtcttgt acctgtgaca tactagttct tcctttcaaa aaagggtaca ggttcttgtt
    11041 gaaaccagta cccttttgtt ttatagattg tgcagcttat aagagagtgt catcagactg
    11101 tcgtccaggt gcatggtcac gacctgtgca ttttccactg ccagatcaca gtggctgaaa
    11161 ttccacaagc cacgcacacc cagagagacc agccgctctg ccacagtctg cgccgactct
    11221 ttcggcagac acagcactgc cgcagccggc tgctgtgccg cacaaaatgc gtccagcgca
    11281 tccatggact ggataggcag cccggcaagc tgtctgccga tcagttccgg cttgatgtcg
    11341 aaaatcccca ccagccgaaa gccacgctgt tcaaagcgga cattttcaga gacgaccttt
    11401 cccaggctgc cggcacccac tacgatcgcc gggagctgtt ctgacaatcc catgatacgc
    11461 tgtagatttt cccgcagctc tgccacggaa tagccgctgc cctgctgtcc gaaacagccg
    11521 aaacagcaaa gatcctgccg gatctgtgat gccgtcatgc ccagccgccg tcccagttcc
    11581 cgggacgaag tacggacctg tccgctgcgt tccagttctc ccaaaagccg gtagtaccgg
    11641 ggcagccgcc gcacaacgga cgatgatgcg tcatattttt gtgtcaaacg aatcgctctc
    11701 ctataaaccg atttccattc tgcaaagagg tgtatctgtg cggatatgcg cttacgccat
    11761 cagctttgcc aaacgctcgt tgatcgcctc caggaattcc ttggagttga ccttcttctt
    11821 gttttccagc ttggagatca gatacaggtc accggtcatg atgccgtcct cgatggtctg
    11881 gatggttgcc ttttccagct tgtctgcgaa agccatcagt tccggcagct tgtccagttc
    11941 accacgcttg cgcattgcac cgctccatgc aaaaatggta gcaacagagt tggtagaggt
    12001 ctcctcgccc ttcagatact tgtagtagtg acgctgtacc gtaccgtgtg ctgcctcata
    12061 ctcatagatg ccggcaggag aaaccagaac ggaagtcatc attgccagag agccgtaagc
    12121 tgtggaaacc atatcagaca tcacgtcgcc gtcatagttc ttgcacgccc agatatagcc
    12181 gccgtgagaa cggatcacac gagccactgc gtcgtcgatc agggtgtaga aatattcgat
    12241 gccggcagcc tcaaactttt tcttgtactc gttgtcgtag atctcctgga agatatcctt
    12301 gaaggtgtgg tcgtacttct tggaaatggt atccttggtt gcgaaccata catcctgatg
    12361 cagatccaga ccgtagttga agcaggcacg tgcaaagccg gcgatggagt tgttcttgtt
    12421 gtgcagaccc tgtacgatgc cgtcggtatc ctcaaagttg aagatcagtt cacggctctc
    12481 gttgccctct gcgtcagtaa ccaccagttc tgccttggaa cccttcttta ccttcatttc
    12541 ggtattcttg tacacatcgc cgtaagcgtg acgtgcaata gtgatcgggg ctgtccaggt
    12601 cgggatatac ggttcaacgc cctttaccag gatcggggta cggaatacgg tgccgtccag
    12661 gatggcacgg atggtgccgt tcgggctttt ccacatctgg gacaggttgt attccggcat
    12721 tcttgccgca ttcggagtaa tggtagcgca cttgacagca acgccgtact tcttggtagc
    12781 gtttgcactg tcgatggtca cctgatcctt ggtcttttca cggtgttcca gacccaggtc
    12841 gtagtactca gtcttgaggt ctacatacgg ggtcagcagg atgtccttga tccactgcca
    12901 cagaacacga gtcatctcgt cgccgtccat ctcgaccagc ggcgttgtca tttgaatctt
    12961 tgccatttga ttttcctcct caaattgcac gcattgtctt tactcattgc cttatattat
    13021 agcgtttctg ccggagcgat acgatgcccg gcttgttctg ccaacgcaga aattttgtca
    13081 tattccgcag ccgtcacaag gatcagctgc aaatcatcgc tgtcaatgtc cacaccgcag
    13141 agcagcattc cctgctgccg gagcagtttg ttgcaggcgg cgcaccattg gaacacgtcc
    13201 gcatcctccg acaagtccag tccggaaaag tctagttttt caccgccgtt cagctgggat
    13261 actgcccaga gaaagtcctc cagctccgag ctgctgtcca cgccgatcag cgcatgggct
    13321 gcctccagtt catccgccat ggcgatccag cacagggtgt ccatgtcgca ggatgcggcg
    13381 tctatgccac gttcggcaaa tcgctccgca aactgcgccg catacgctgc ccgatcattt
    13441 cggcagcgtt ccaggctgtc atgcaccgtg gaattgttat ggctgatgca gtctgcgatc
    13501 gccagaatat atggcacctc agatacctgc tccggtgctt ttttcttttt gaagaatccg
    13561 aacatttacg caaacaccac ccatcgcaaa acgcccagca ccaacaggtg cagcggatag
    13621 aacacataga aaaaccactt gtgaaacggc tttttgctgc cgccttcgcc gttgtagcag
    13681 tactgtatca gcggtatcac cagaaagata cccaactgga acagaccact ccacatgggt
    13741 tctgatgtca gcacactcat acagcacacc agtgtaatgc cgccaaacga cagccatttt
    13801 tccctggggc tctcccgata tcggaaaaag gtcagcgcat acagcacatc gaatatcgcc
    13861 cagtcaccga atacggacag caggcacagc ccaaagatgt ccagccactt caccgaaaga
    13921 gaacactttg ccttgtccca gaaccagatc gccagcaggc tgaggaacag cgtgtagatc
    13981 acgccgaatg ccggacggat gccgccactt tcgaaatagc tgaacggcag ccacgagatc
    14041 acggcgaaaa tgcccagccg catggcgtat ttctttacgt tccgggtgta gtgataccct
    14101 tctgccagca tatacgccat agtcggtccg gtcagccgtc cgatgacgtg catgacctgt
    14161 cccaacagcg acgccgtggg aacaaatgcc caggcgatat ggtcgatgag catggcggca
    14221 atcaccagat atttcagctg gttgcggttg agaaatcctt ttgtgtttgt cattccggct
    14281 tatccttttc ctgccgcagc tgccgtatca gccttctcac cagatgcaca ccgccggcaa
    14341 gcaaaaacag cagtaccagc agtccgatcc cggaaaccgg gcgtttccac agcagttcca
    14401 gataatctgt tatgtccctg tcgtcaagca gagcaatcac acaaaacacg agaaacactg
    14461 caaacagttc ctgctgatag caattccaga acttccgcca ctttccgctt ttctctgtct
    14521 tttccgaatc taccacttac ttgctcagct tgtcacgggt ataatccagc agaccgcctg
    14581 ccaggatgat atccttggtg cgtccggtca gctcgcacag taccgggatc tcagcgccgg
    14641 tggtcttgtt gaccagtgtc agcgtcgttt cgccagcctc aacgtgcttg cggatgtcag
    14701 ccagttccag aacatcgccc tgggagatct tctcatagtc tgcctcgttg acaaaggtca
    14761 gcggcagaat gccggcattg atgaggtttg cacggtggat acgggcaaag ctcttcacca
    14821 gaactgcctt gacgcccaga tacagcggtg ccagtgcagc gtgttcacgg gaggaaccct
    14881 gtccatagtt ggagccgccc acgataatgc tctgtcccat ctccttggca cggctcggga
    14941 acgcctcatc gcaaacggtg aagcagtgct gcgaaattgc cgggatgttg gaacgcagcg
    15001 gcaggatctt tgcgcctgcc ggcatgatgt ggtcggtggt gatgttgtcg ccgaccttca
    15061 gagagcagcc agcctcgatg gttgcctcca gcggagcagt ttccgggaac ggcttgatgt
    15121 tcggtccacg caggatctct accttgtcca tttcctcttc cggtgccggc agaacgatca
    15181 tgttgtcgtt gacactgaat ttttccggca ggacgaagtc cggcatctcg cccagttctc
    15241 tcgggtcact cagataaccg gtcagtgcag aatatgcagc cagttccgga gaaaccagat
    15301 aaatctgacc gtccttggtt ccggagcgtc ccaggaagtt ccggttaaag gtacgcaggg
    15361 agataccgcc ggagttcgga gactgtccca tgccgatgca cggaccgcag gcgctttcca
    15421 gaatacgtgc gccggcagcg atcagtgtac ccaggatgcc catatctgcc atcatgttca
    15481 gcacctgctt ggaaccagga gcgattgcca gagaaacatc cggatttaca gtcttgccct
    15541 tgaggatgtg cgccaccttc atcatgtcca gcagagaaga gttggtgcag gaaccgatgc
    15601 agacctggtc gatcttcagc ttgccgatct cagccacggt cttgacattg tccggagaat
    15661 gcggacatgc agcaagcggc accagttcac tcagatcaat gtgcagttct tcgtcataaa
    15721 cagcgtccgg atctgccttc tgctcgctcc agacttcgcc tctgccctgt gcctccagga
    15781 actgcttggt cacttcgtcc gacgggaaaa tagaagtggt cgcacccagc tcagcaccca
    15841 tgttggtgat ggtggcacgt tccggaacgg acagagtctt gacaccctcg ccgcagtatt
    15901 cgatgacctt gccgacaccg ccctttacgg acatccggcg cagcacttcc aggataacat
    15961 cctttgcagc cacccacgga gacagcttgc cggtcagttc tacctttact accttcggac
    16021 aggtgatgta gtatgtaccg ccgcccattg ccactgcaac gtccaggccg cctgcaccca
    16081 tagcaatcat gccgatgccg ccgccagtcg gggtgtggct gtcagagccg atcagggtct
    16141 ttcccggaac gccgaaacgc tccagatgta cctggtggca gataccgttg ccgggacggg
    16201 agaagtagat gccgtgtttc tttgcaacgc tgccgataaa acggtggtca tctgcatttt
    16261 caaagccgtt ctggagtgtg ttgtggtcga tataagccac gctgcgctca gtgcgtacac
    16321 gaggtacacc cattgcctca aattccagat atgccattgt accggtggca tcctgtgtca
    16381 gtgtctggtc gatgcggatt ccgatttcag aaccctttac catttcgccc tctaccagat
    16441 gtgctttcag gatcttttcc gtcagggtca gtcccattca atatcattcc tttcgtagtt
    16501 ggcactgcaa atacacggca agccatccat ttacaatacg attcttatac cgtattatta
    16561 tagcaaaaaa caggcgctgt tgtcaagtct gcggcgactt tgaaaagata aaagaaaaat
    16621 ccggatttgc ttacaaataa aatccgccgc tcccggcagg gtgcaaaaac agcccgaagg
    16681 tacatttccc tcgggctgtc gtattttatt ccgtctcgga aatatggtac acatggtcgc
    16741 cgctgagacg cttttcatac tcgtcgtgag gcagcggacg gtcgaacaga aatccctgtg
    16801 ccatacagca gttgatgcca cgcaggaatt ccgcctgttt ctgggtctcc acgccctcgg
    16861 caatcacttc catatccaga tcgttcacca tgtttacgat gttcttgatt acgatcacat
    16921 cgtttttccg gtgcttttcc tgtttttcca tgttgttgag gaaggattta tccagcttga
    16981 tgatatccac attcagatct ttcagcaggt tcagcgaaga atagcctgtg ccgaaatcgt
    17041 cgatggaggt gctgacaccg ttttcgtgca tggtctgcac aaagcggagc agtgtctcat
    17101 agctttcata gccggacatt tccgtcagtt ccacctcaat gtatttggca gagacattgt
    17161 actttttcag cactgccaag atctcttccg ccagattcgg atttttcagg tgcatcttgg
    17221 agaaattcac ggagatgcgc accggttcga ttcctctcga cagccagtcc tggatgttct
    17281 ggcagacacg ttccaataca tagaagtcca gcttgcagac gctgccctct cgttccagca
    17341 ccggaatgaa gtccatcggc ggcaccatga caccgttccg gaaccagcgc accagtgcct
    17401 cgcagccgca gagctggttg tccgaaaggg tcaccttcgg ctgatagaac acctcgaact
    17461 cctggttttc cagagcattg gggaacatct gcgacacttc tttctgacgc agtgcctttt
    17521 ccagcatcgt gtcgtcaaag tggagcatat ccgtctttcc ggacatcttt gccacgtgca
    17581 ttgcagtcga tgcggcgttg attacctcgc ctgtgttctg gttttcccgg atatcgtata
    17641 ccccgattcg ggagcgtaca tcaaaggtga tctgcttgtc ctcgttggag accgtcacat
    17701 actggtttcc gatctttttc agcaggttgc ccatatgctc ttttttcgcc agcagcacaa
    17761 agttgtcacc gcccatccgg gcaaagaact cgtctttctc cagcatattc tgagacagac
    17821 gcacaaattc acgcagtacc acatcgcctt ttcgtgcgcc taccgcacgg ttgatgtagt
    17881 tgaaattctt gatgttcacg aatgcgcagg cgtagtcttt cagtttgccc tgcgccagaa
    17941 gtttgccgcc ggtctcgttg atgccacggg tgttcagcgc accggtcagc aggtctgtta
    18001 cggaaatgca ccgcagcagc tgtaccagct tgacccgttc cagcatcaga aagatctggt
    18061 gagacaggaa ctggagcatt tccgcctggg gctgtgtcca tgcggtatcc ttttccggat
    18121 aaaagtaaaa gacagcgctg cagttttcgt ccatctgcac cttttcttca caaggcggcg
    18181 catcaaatcc cttttcgctc tcatagatca gataacagtc gtcctgccca ttgggatcca
    18241 tcaccgattt tgccgcataa aaccgcagtt caaattttcc cagcgaaaaa acacgtgcca
    18301 ctttctgcat ctgatcctgc agcagtctga tcagatcatc cacagttttc acattctttt
    18361 gcaacgaatc aaaaagctgc tggtaactct ttacatgaaa attcatatcc ataccaatca
    18421 cctcattcag cgcctgtttc atgtccgtac agagcgcaaa acaaacggaa tacaatttct
    18481 acccactatt cccacattat ttctatgata aaaactatat catatttttt cggaattgtc
    18541 aatcagacac ataaaataaa ttgagcggaa tcaaatattt gtgcgttatc acgaaaagac
    18601 tttctcattt ttgttggttc ctacaaattt gacaaatgag aaaaaatgcg gtacactgat
    18661 agacagaata aatcgaatgg atgtgaaaac catgcagact gtactgcaaa acctttcttt
    18721 cagcctaaac gccaccattc ccgtattttt gatgatggta ttcggcggtc tgatgaaaaa
    18781 agtaaacctg ttggacgacc acaccaccgg gaaattgaac cagttcgtgt tcaaggcact
    18841 gctgccggcg ctgctgttca tggacttgtc ccaggcggat ttccgctcgg tctgggacgg
    18901 aaagttcgtc ctgttctgtt tctgcgccac ctttctcagc atcggcattg cctttctcct
    18961 gtccctgcca cgcaaaagct ggtcggagcg tggcgagatc atccaggcat cctaccggag
    19021 cagcgctgcg atcctgggca tcgcctttgt caacaacatc tacggtcacg ccaccatggc
    19081 ggcgctgatg atcgtgggaa ctgtgccgct gtacaacgtg gcggctgtcc tcatcctctc
    19141 cctcactgcg ccccatgacg gcgaaaagcc cagcgccaga aagctgctgc tgcgcacgct
    19201 ccgggatgtg gcgaccaatc ccattatcct gggcattctc gccggaatgc tctggtcagt
    19261 gctgaaaatc ccgcagcctg tgatcctgca aaaatccgta tcctacctgg gcaatatggc
    19321 aacgccgctg gcgctgatcg ccctgggtgc gtcgttccag ctgaagagtg ccaagggcaa
    19381 gctggggctg acgctgggca tctctgcggt aaagctgttt ctgttctgcg gtctgtttct
    19441 gccggcggcg gtctggctgg gattccgcac ggaaaagctc atcgccattc tcattatgct
    19501 gggcagcgcc accacgtgca gctgctttgt catggcaaag aacatgaaac acgagggtac
    19561 catcactgcc tgcaccgtaa tgatgaccac cctgctcagt gcgttctctc tgacgatgtg
    19621 gctgttcgtg ctgaagagcc tggggtatat ttgatgatat gagaaacggg cttgccgcac
    19681 cattcaaaaa gtgcagcagc ccgttatttt tttaccctga ttttctccgc ctcagacctc
    19741 attctcctgc aattcctgga agaatgtgaa atagtcctgg cgcttgtcgt tggtgaatgc
    19801 gatcgctgtc cggggatgct gattcagata gccggtcagc aggtactgga tgtcatagcc
    19861 ccataccacg ccgtcatttt tcagtttctg catctcatcc tggaggaaac gcagcaccgg
    19921 cttgatgtca tacttcgggt ttttcaaaaa gcccagcagc agttccattg cacagttacc
    19981 tgcgccacgt cccattgccg ccattgtggc atccagatag ctgacgccca tcatcgcaca
    20041 ctctgtggtg ttggcgaatg ccagctgctg gttgttgtgg gcgtgaatac cgatcttctt
    20101 gccgtacttc tcgcctgcct ccagataccg ctgtgccagc tgacgcatct gcagcggata
    20161 gagagagccg tagctgtcca ccagatagat gacctcaacg ctgctcttgc ccagcatttc
    20221 cagcgcctct tccagttcag actcctgcgc cttggaaacc gccataatgt tcactgtggt
    20281 gtgatagccc ttcttcgcac aggtctcgat catctcgatg gctgccggga tggtgttgat
    20341 gtaggttgca atgcggacaa catccaggat gctgtccttc ttgtccggca ggtcacgagc
    20401 cacgttggca cgtcccacgt ctgccatcac tgccagctgc atcttcgggt cgagaatatc
    20461 ccccagcacc ttccggatag cgtcctcgtg gcagaacttc cacttgccga acttggactc
    20521 ctcgaacagt tccgggtctg ccagatagcc gatttccata atatccacgc ctgcacggag
    20581 gttggtcaga tacagcttgc ggacaaaatc gtctgtaaac tcgaagttgt tcaccagacc
    20641 gccgtcccgg atggtggcat ccagcacttt tatgtcagga cgataggaga tcatcgtgcc
    20701 ttttatcata tcactcattg tacattcctc gctttctgct tttcccgtgc tgcttttcgt
    20761 cagcacagta cttatattat accaaaaaat gcacctttct gcaagtattt ccgccttttt
    20821 tctgcataac ctgcataaat cgtgattttc gccaatccgc attcccaaaa actgttcagt
    20881 ttgccaacga aaaaggactg ccctgcgaca gtcccctttt tcagcgtttc ttccaatcgg
    20941 tgcgttccgc cttatccggc ggtgtaatat tccggcatct ctgtggtcag atctttcagt
    21001 atgtagtagg tatagccatg ttccccgtcc gtagaagtgt aaaactcgat cacgtttccg
    21061 ctgtccccga atacggcata gacatccgtt cccgggtcgg cgcagttggt ctgcagttcc
    21121 tctgtcggca caggtgtctg ctcataaagg gaaacagtca gacattcact ggagtccgcc
    21181 gcctttccca gcagctttgc gtcagacggc acagtcgatt ccgaaccgta aaacgccacg
    21241 ccgtagtaac acgtcccgtt gtaaacaatg gcgttcatcc cttcgccctc tgccatatct
    21301 gtttctgcga tgctggtctc tgccggtttt gttttcgccg gcttggtctc aacgctctcc
    21361 tgcgaagatg tgtttccgcc ggcgcagccc gacagcagca tcgttccaat caatccaata
    21421 caaaacagct ttttcataaa gatcacctca agtcgtctgg tcatttttgc tcctatgcca
    21481 tcagaatcct ttcttctgat actgctattg taccacattt tctacacaat tgcaatatta
    21541 tttttacttt ttttacgcga aaacaaaaga tttatttttc tgacagccca tccagtcgga
    21601 tattggaaat gattttcaaa aagattccat aaaatccaca tgaaagtatc tttacaatgt
    21661 tctgcaaacc aggtataata aaaatagtac actttcagga ggtatcatat gtttttttct
    21721 gaaactgacc tatccgactt catcgaggaa aatgacgtaa aattcattcg catgaccttt
    21781 tgcgacacgt ttggaaatat gaaaaatatt gccatcatgc cacgggaact gcaccgtgcc
    21841 atcactgacg gcatcccgtt caacgccacc ggactgctgg aggcatccca tcagaacctg
    21901 cttctgaaac cggacacctc caccctgtcc atcctgccct ggcggcccca gtcgggacgt
    21961 gtggtccgct ttttctgcaa gctgtatcac atggacggca caccctatga gggcgacctg
    22021 cgccggaatc tccgggagac catgaagtct ctccagaaac agggctatca gtgcgaaatg
    22081 ggaacacgct gtgagttcta tctgttcgag accgacatgg caggaaagcc cacccggatt
    22141 ccctgcgacc agggcggtta tctggacgtt gcaccgctgg acaagtgcga aaacacacgc
    22201 cgtgagatct gtctgtccct ggaggagatg gggctgaatc ccaccacatc ctgccacaag
    22261 cacggccccg gacagaacga gatcgacttt gcgtgcagca atccgctgac cgcagccgac
    22321 aacatggcac actataagac cgttgtcaag accattgcgg cacagaacgg tttctatgcg
    22381 tcctttatgc cgaagccgtt caaggactgc agcggcagcg gactgaagat caccctgtgc
    22441 atcaagaaag acgacaagag cattttcggc acgagccaca aagacctgac accggagggc
    22501 agagccttta tcgccggaat ccttaaccgt gccagagaat tcaccatgtt ctccaacccc
    22561 accatcaact cctacgaccg gttcggttat cgtgccgcac cgtcccggat caactggtca
    22621 gaagagaacc ggattccgct ggtacagctg ctgtatgcgc cgggcagaga cggctccatt
    22681 gagttccgct ccgctgatgc gtactgcaac ccttacatca catttcagat gctgctgagt
    22741 gccggcatgg ctggcatcca gaacggcgaa gagctgtacg acaacatgaa cgctgccaac
    22801 gagaactccg ccattcccac gctgccccat tccctggaag aatccatccg gctggcgcag
    22861 gagagcgact ttgtccgcag caccgttccc gaggcaattc tccacgactt ttccgccgca
    22921 atgcagaaag aagtggacgc atatcatctg gcaaaagacc cggaggcgtt ttgctttgag
    22981 cggtattttt aagcagccgg caagctgtaa tctgaaaaag aggagggaac acaacggtgt
    23041 attccgaacg cacactgatt atctccggct ctgaaaaagg gctggacggc gtgatcggct
    23101 ttttgaaaac ctgtggctgc ggctccgttg cagtcgtctc cagcggcagc gaggcaagac
    23161 gcatgattcc ccacgacgat tatgatttga ttctgattaa cacaccgctg aaagacgaat
    23221 tcggtcacca gctggcggtc aagctctgcg aatccacctg ctccggcgtt atcctcatct
    23281 gcaaggcaga tctggcggaa gagatggtca accgcaccgg cgactacggt gtctgtgtgg
    23341 tatccaaacc actgaacaag caggaactgg tgaaagcgct ccgcatcggc agcgctttcc
    23401 ggcaccgtct gctgacgatg caaaaggaga acaacaagct ccgggcaaag ctggaagaga
    23461 tgcggtatgt ttcccgtgcc aaattcctgc tcatctccga acagggcatg accgaagagg
    23521 cggcgcaccg ctacatcgaa aaaagcgcca tggacacccg gcgcacccgg aaagaagtgt
    23581 ccctggaagt tatcgcaaaa tacgaataaa catcgtttgg aaaaacgaaa aaccgcagcg
    23641 gaaagaaaaa aactttccgc tgcggtttta tttttgtgat tttataagtc ttactttgtg
    23701 ccgaaaatac ggtcgccggc atctcccaga cccggaacga tatagccctt ttcgttcagg
    23761 ctctcgtcca cgccgccgca gtagatgtca acatccggat gcgctgccag cagagcgtca
    23821 cgaccctccg ggcaagccag gatgcacagg aacttaatgc tcttgacgcc catttccttc
    23881 agatccttga tggctgcgat cgcagaagta ccagttgcca gcatagcatc gatcacgata
    23941 acttcacgct ctgcgatgtc atccggcatc ttggaatagt acttcacggc atcgtgtgta
    24001 cccggttcac agaaaatgcc gatatgaccg atctttgccg ccggaatgag cgaagtaacg
    24061 ccgtctacca gtcccagacc tgcacgcagg atgggaacga aagccagctt tctgccggag
    24121 atcaccttgg tcttagcgat gccctggtgt gtctcgatgg tgacttcctt catcggcaga
    24181 tcccgggtcg cctcataaca gatcagcatt gctgtctcag aggtcagctc ccgaaattcc
    24241 tttacgcctg tcattttatc ccgcatcagt gagatcttgt gctgcaccag cgggtggtct
    24301 actacatgaa tccctgccat gttcttatcc tcctgttacc attctttttc cagttcagta
    24361 atcatattca cacggttctc gtgtctgccg ccctcaaaag gcgtatccag gaaaatctcc
    24421 accagttcca atgcgagacc gctgcccagt gtccgggcgc ccatgcacat tacattggcg
    24481 tcgttgtgca gacgggtgta cttggtggaa aacgcatctg cacacagtga tgcacgaatg
    24541 ccccggatct tgtttgcagc gatcgacata ccgattcccg tgccgcaaat cagaatcccc
    24601 atgtcacccg gatgagatgc caccgcagta ccgactgcct tggcgatcac cggataatcg
    24661 gctctttcgc cgttacagcc gcagtcctcc acttccagct gccgttcttt cagcagcgca
    24721 atgacctcct gcttgcgttc atagccggcg tggtcacatc caatccaaat tttcatgttc
    24781 ttctgactcc tttgtcttga gatacttcta ttatagcata tttaccggac aattgcaagg
    24841 tgtattttcc aagatacacg aaaatcactt tgcccgctgt ttctcctgca agtccttttc
    24901 cgctttcacc agctgcaaat actccggcat cagttccgcc ggggcctgcc actgtgcctg
    24961 cgccgctgcc aggcagatgc ctgtgccgtt ttgcagacgt gtcatgggag atgcgatcgt
    25021 cagaccctgc tccgcccgga acagttccac tgcatcgccc gccagcagca ccggctgttc
    25081 cagtgatgcc agctgctgcg ccagttctgc cgcaggctgc attccgtccg gctgcattgc
    25141 tgtgatctgc gtgccgtcac tctggtacag tccggcatag accagagact gtctcgccgt
    25201 catgaccgca cagatcacgc cctgccagga cagattctgc cacgccagcc cttccagcgt
    25261 agagattccc acgcaaggga tctgcagggc gtaggacatg gcttttaccg ccgcaatgcc
    25321 gatgcgcagc ccggtgtagg aacccggacc tttcgccgca gcgaaaccgt ctacatcctg
    25381 ccagttcagc ccggtgtccg ccagcagacg ctctgccaga ggcaggatca cctgggaatg
    25441 ggtacgtttt gtataaaccg tctgctgcgc cagcaggcag cggctttcgc tgtcatacag
    25501 cgccgcagcc gctacttttc cggaagtgtc aatccccaag attcgcaaag cgctcatctc
    25561 cttctactgt gatctgtctg ccgtcatcgg aaagccgctc aatggtgatg cggatgcagt
    25621 tctccggcag ctccgatgcg atattctccg accactcaat ggcaaatacg ccctcatcca
    25681 ggggatagtc atagaacccg gtggattcca gatccagtgc gccctcgatg cggtacatat
    25741 caaaatggta gagcgacnnn nnnnnnntag agcgacagtc cgtcgccgtg gtattcctgc
    25801 acaatggcaa aggtaggcga agaaaccaca tccggcagtc ccatgccctt tgccagtcca
    25861 cgggtaaagg tggtctttcc cacgccgaga ccgccgaaat aggcgatcac atctccggcg
    25921 tgcagctgtt tccccagctg ttccgcaaag gcaatggtct ccgccggcga tgccgtcgtt
    25981 ttctgtattt tcaacacgtc tgttccatct ccttagaaca atccggtaat gttgccgtcg
    26041 ttgtcgcagt cgatcttcaa agccgacggt gccttcggca gacccggcat ggtcatgatg
    26101 ttgccggtga gaacaaccac aaagccagcg ccgttggaca gtgtcacgtc acggaccgtg
    26161 atggtgaagt gttccggctt gcccaacttc gcagcatcat ccgacaggga gtactgtgtc
    26221 tttgccacgc ataccggcag attgccgcag cccagagcat tgatctgtgc cagcgctttc
    26281 tttgccggag cagtaaaggt cacgccgtct gcacggtaga tctcctttgc gatcttttcc
    26341 accttttcct ctacggaaag gtctgtgtca tacagtacgt ggaaattgga cggcttggtc
    26401 tcgatggtct tgcagacctt ttctgccagt tccttgccgc ctgcgccgcc cttagcgaac
    26461 acttcggtca gagcgaattc tgcgcccaga tcttcacaga acttctggat aaagttcagc
    26521 tctgcatctg tgtcgcactg gaaacggttg atggcaacca ctaccggtac gccgtacttc
    26581 ttcatattct cgatatgc
//
LOCUS       DAWENO010000007        25329 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_788_length_25329_cov_12.185447, whole genome shotgun sequence.
ACCESSION   DAWENO010000007 DAWENO010000000
VERSION     DAWENO010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 25329)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25329)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25329
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_788_length_25329_cov_12.185447"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    462..471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7223..7232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21403..21412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5996 a         6773 c         7183 g         5347 t
ORIGIN      
        1 cccggctacc gcaccggctg taagctggct gtggtggacg agacgggaaa ggtgctggat
       61 accggcgtgg cacacatcac cctgggaaag ggcgcatccc tggaaaaggg caaggatacc
      121 atccggaaga tgctgcggca gcaccatgtg accgccgtcg ccatcggaaa cggcacggcg
      181 tcccgggaat ccgaggctgt ggtggcagaa ctgctgaaag agctgcccta ttctgtggcg
      241 tatatggtgg tctctgaggc tggggcatcg gtgtactctg cgtccaagct ggcggcagag
      301 gagttcccgg agtatgacgt ttcactccgg agcgccgtgt ccattgcccg gcggttacag
      361 gacccgttgg cggaactggt gaagatcgac cctcaggcga tcggcgtggg acagtatcag
      421 cacgatatgc ccaaggcgga gctgtcggcg gcgctggacg gnnnnnnnnn ntgcgtgaac
      481 cacgtgggcg tagacctgaa caccgcctcg ttctccctgc tgtcccacat cgccggcatc
      541 aaccagacca tcgccaagaa catcgtggcg taccggacag aaaacggcgc attcaccgac
      601 cggaaacagc tgaaaaaggt ggcaaagctg ggacccaagg cgtatgaaca gtgcgccgga
      661 ttcctccgtg tgccgggcgc aaagaatcct ctggacaaca ccgccgtgca tccggaaagc
      721 tatggcgcag cggagcagat tctccgggaa tgcggctttc agctggcgga cattgccgga
      781 caggaccgca gcgagatcac atccattgcc aagcagcacg gtatcgcagc cattgcgaaa
      841 aaagctggcg tgggtgagcc gacggtaaag gatatcatca aggagctggc aaagccggga
      901 cgtgaccctc gtgacgaact gccgccgccg ctgcttcgga gcggtgacat catggagctg
      961 aaagatctga aaccgggcat ggaactggtg ggaacggtgc ggaatgtcat cgacttcggc
     1021 tgttttgtgg acatcggcgt ccatgaggac gggctggtgc atatttctca gatctgcgac
     1081 cggtttatca agcatccgct ggaggcagtc aaggtcggcg aagtggtaaa ggtctgggtg
     1141 ctggatgtgg acttgaaacg caagcgcatt gcactgacca tgaaaccgcc gaagaaagga
     1201 taaaggagaa catatgggat ttggaaagaa actgactgcc ctggtattgg cggcggcaat
     1261 gacggctgcg gcgtgcagct gcggcacgga aagcggcaaa agcaccgccg gagaagagag
     1321 cagccaggca gaggaaaagc cggacttccg cacggaaatg cgggacgata tgaccacagg
     1381 cgacatcgtg gcggaaatgg ggctgggcat caacctgggg aacaccctgg aggcgtgcgg
     1441 cgactggatc gacagcagcg gcggcgtgaa cagctatgag accgcctggg gcagccctac
     1501 ggtgactgag gaaatgatcg caggctatgc tgccgccggg ttcgactcgg tgcgcatccc
     1561 ggtggcgtgg tcgaatctga tgggagacga ctacaccgtt gcgccggaac tgctggatcg
     1621 tgtggagacc gtggcgcagt atgtgctgga caacgggatg tacgccgtgg tgaatctcca
     1681 ctgggacaac ggctggatcg ccggattctc caccgactat gacacctgca tggagaaata
     1741 cacccgcatc tgggagcagg tatcggagcg cttttccggt tacggcgacc atctcattct
     1801 ggaatccatg aacgaagagg gctgtttcga cgatctgtgg aatcactaca gcggttcgga
     1861 ggacggcaag gcggaggcat acggcatcgt caaccgtatc aaccagaagt tcgtcgatct
     1921 ggtgcgcagc tccggcggca acaacccgga gcgccatctg ctcattgccg gctatgccac
     1981 cgacgtggac atgacctgtg acgaaatgtt ccagatgccg gaggacagcg ccggacggtg
     2041 cgccgtgtcg gtacattatt atacgccgtc tacgttctgc atcctggaag aggacgccga
     2101 ctggggcaag gcaaagacca cctggggttc gcccagagac cagcggatac tggaaaagga
     2161 catggaaaag ctccagaccc gtttcctgga caacggcatc ccggtcatca tcggcgaata
     2221 cggctgttcc accgggaaca agaccgagga cgccgtccgg acatttctca cggcggtggc
     2281 acgtgcggcg tattcccgtg ggatgtgtcc catgctgtgg gacatcaccg gggtgttcta
     2341 tgaccgcagc agctgtaaaa tgatcgacag ccagctggag caggaactcc gggatattgc
     2401 agagacggcg agaaactgaa aaaattggta aagatgcgct ctgaaaactg ccggaaaaag
     2461 gcgtcagagc gcatcaaaat agcaagttgt ttttcacgca atttttcaga tttagaagaa
     2521 ttgcacaaaa acagaggttg cttttattcg tgctgtataa aaatgtgtgg aaaatgaaaa
     2581 aaacgcttgc aaaaaatcta caaatatgct atgctggtat atggagaagt agggtgcagg
     2641 aaagcactgg tatggcttca tgaagatgtg cgccgactgt gcaattggaa agcggaaagc
     2701 acgcaaatca attttaagaa tgtccccctt gaaaaggggg atgtcagcgt aagctgacag
     2761 ggggataatc ccgacccttg ctctttacgg agcattcacc tcagcacaag gcacactcta
     2821 tgggtgtctt tgcaagggaa gtaattagaa aattgatttg tgtgagcgga aagggggcag
     2881 cagacgactg gcacggcgga ggtacggaaa ccaaggggca gaaaagcttc tctgcacaca
     2941 accaaggcgg cgctatgccg cagaaaatca agaaaggaag aatgataaac atgaagatga
     3001 agaagtttct ttcatctctg actgctggtg taatggcggc aaccaccgta ttcaccagtg
     3061 cgctggtaac accggttatg tcgtttattg cgactgcggc tgatgctgac gcaatcggta
     3121 cagcaacttt agtaggtgta gtcggtgcca atacgaattg ggaagctgat gtaaatgcaa
     3181 caagtatcac tggcgacggc gaatacaccg tttcctggaa tctggatagt gcatcagact
     3241 ccattagttt cctgacagtt cagatcagta aggcaggaga caccgaaaac tttaccactg
     3301 atacatttaa ggatctgaag gtatctctga gttccgtttc catcaacggc gaagaggtag
     3361 cagatgcggt aaaggacgat aagattgtag atactgcttt ttatgaaggc ggtaatgctg
     3421 gtacgactcg tatctatctg aacggcagct gggcaggtaa tcatgctggt gcgctggata
     3481 acttcgatac aactaaggta gagaacggta tctctaagat ttccgttacc tttactgtag
     3541 aaggcaccgg caagaccacc acaaccacta ccagcgagac cactgcggaa accacagaga
     3601 ccaccgtttc ttccgcagtt tcttctgatg tttccagcga gacatctgaa tctgttgtaa
     3661 ccactgcatc tgcatcctct gccgagactg tgacaaccac aactgctccg gcaattaagg
     3721 acgtagaggc aaagatcacc accggcaccc agaagggtaa agacggtgat atccaggcat
     3781 atgcagaatt tgacccgaac ggcgcaaagt ctgcaacact gtattatacc ataacttcca
     3841 aggataccga gtccagcggt gctttcggca catacaacgg cgattggttc caggaagaat
     3901 ttaaggcaag cgttggcgca aatggtgcct gcacagcaga ttacaccatc ccgtccaatg
     3961 ttggcaagac cgtaaaggca atgatctttt atccggatgc aagcagcgtc aagatcgaca
     4021 aggtggttct ccactatgat tccgctgtta ccacatccgc aacaaccacc agtgtcacca
     4081 caaccactga ggcagcaaag tacaccaagg tactgactcc gaaggctgag gaagacaagg
     4141 gtactgacgg caaggcaagc aacaccaagg ttgagttcga cccgatgggc gcttacaagg
     4201 caatcgctta ctacaccgta aagacaaatg acaccaacac cagcggcgct ttcggcacat
     4261 ggaacgacag cctgccggag ggtgaagagt ggcagagcga ggaattcaag gatctggccg
     4321 ttccggcaaa caagcaggtc gttgtttcct acaacattcc gaagactgtc ggcgaaaccg
     4381 tgcagttcat ggtttattac ccgaagttcg gtgatgtgac catcgacaag atcgttctgt
     4441 gcttcgacga agatccggat gcaacaacaa cctccgtttc ttccaccaca gagatcaaga
     4501 ccactgcatc cagcgtgtcc tctgagacag agaccactgc atctgcaacc accaagggaa
     4561 cagagtccag tgcatctgag acaacaaagg caacagagac cagcgcaacc tctgcaacta
     4621 ccgagactga caaggcaaca gagaccagcg catctgcaac cacaaaggat acagaatcca
     4681 gcgcaacctc tgcaaccacc aagactgaca aggcaacaga gaccagcgct accgaaaagg
     4741 caaccactgt tacaagcacc accaagcagt ctgcaaccac aactgcaaca gatgcaccga
     4801 agggcaaggg cattgtcatc gacatcggcg aggctaacgc agagccgggc gaagagggcg
     4861 ttgaactgcc gttctatctg cagaagtata tcccgtctga gggctttagc tttgcaattg
     4921 atgtcccgat ggtaactgca aagatcctga gcatcaacaa gaccgaagtt tccaaggacg
     4981 gcgtggatga atatgtatcc ggttctgtcc gcagaacttc caacaccgca ggctttgagg
     5041 cattcggcgc tgacgaatcc gcagctccgg aagatcgctg gatgtacttc atgtggactg
     5101 tttccgccgg cactgcaacc atcccggatg aagaaaacgg cgcaagcaac ttcatgaata
     5161 tgtacttcga catcgctgac aacgcagcag atgttgcagc agaatacggt ctggaactcc
     5221 agacagatgc tgacggcaat acctactact tcttcccggt caactttgca cagtaccaga
     5281 aggtaaactg ggacttctcc ggcacagcag gcagcgcata catcatgccg aaccagcagt
     5341 tcctggacga cagcagcaat gacctggcta ccacagacgt tacttactac aacggcggtt
     5401 tccgtgtata caccgacaag aaggatgtta ctacatcttc taccgctgat acaaccacat
     5461 ccatcacaac cacaaaggca accacaacta ccgaagaaaa ggatatgccg atcaacctgg
     5521 acatcgatga ggtttatgta tatccgcagc cggctggcga aaacccggat tatattggca
     5581 ttgaagaaga actgggcgta aaggttattg ccaaggaagg cgtagacacc aagagctatg
     5641 gtatcagcgg tgcagtcgca ttcccggatg caaccgcaaa gctgttcacc attccggacg
     5701 gcatggagag caaattcgca gtcaacgact tcggttccag cggcagaccg aacatgatcg
     5761 actttgctgc ttataagaac ggcacatcca ccatcaccga cggcagcgac cgctggatcc
     5821 gcttcatcga agcattctcc aacgacggtc cgcaggatcc ggctgagctg gatcgtatct
     5881 tccacctggt attcgatgta ccggatgcag ataccgtaca ggcaatcgca gacgaatatg
     5941 gtctgacact tcagaccggc gagtatgacg gcaagactgt tcagtactac gagttcccgg
     6001 taacctgggc tccggacggc attgaccaga tgcccagcgg cggcaccatt gttgatgttg
     6061 agcgctttaa gtatctgaac ggcgagggcg tgaccggtga ggatatcttc gacgaccagg
     6121 ttggtctgaa ggatggtgcg atccgtgtta tcatgaagga agtggaagat accacaacca
     6181 caaccacaac tgtaacaact accactacta caaaggcatc cacaaccaca acaaaggcat
     6241 ctgctacaac aaccacaaag gcatccacaa cttctacaac aaaggcatct gttacaacaa
     6301 cgacaaagga atccactacg acaacaaagg cgtctgctac aacaactaca aaggcatcta
     6361 cgacaacaac agaagaagcg acttctacaa caacaaccga aaagacaacc gtttccgaga
     6421 cttccggaac aacaactaca aaggcaacgg aatccgaggc tccggtaagc acaactactg
     6481 tagaatcgac taccacagag agaggcacat tcaccaagaa gactacaact gtgacaacga
     6541 ctacagaaga tgcaaactac accaaggtct ccagaccgac cggaacaacc acaacagaaa
     6601 ccacaacttc tgctgagaat accacaactt ccagcgagac aaccacaagc actggttctt
     6661 ccagcgatac tgttacttct accacaacaa ccgagactca gcctgccggc aagccggatg
     6721 ttaaggtaaa ggaagatgca aacttctacc tgtctgagga cactcgtgca ttcgacccgg
     6781 cagatctggt agagagcgca actctggacg gcgaggacat tctgagccag ctgaccttcg
     6841 agtacgcttc tccgaaggat atgtacgatg cactgcacgg cgatatcgct tatgttgcat
     6901 ctgacttgaa ggttctgtac aacggcgaag aagtcggcac tgctaaggtt tacatcggca
     6961 tcaagggtga tacggacttg aacggtaagg ttgaggcaac tgatatgttc tacaatatgt
     7021 actatcaggc aagactgggt gccggcatca aggaaggcgt aactctgttg gagaacaacc
     7081 aggatgagaa tctggagaaa ctatcttact tcttgaccga tatcgacacc gagagcaagg
     7141 aaggcaagaa cactgccgac aagtccatca ctccgactga cctgttctat cagatgtact
     7201 acatcgctct gaagggcgca ggnnnnnnnn nnactgtttg gaccgatgtt tgcccggatc
     7261 tgaagaacct gcagggttcc tgctggtatg aggcaaagta agccaaaccg aaacagaacg
     7321 tgaaaacgtt tttgacagcc tggatatgac atcataagaa aacccgatgc gggagcaatc
     7381 ccgtgtcggg tttttgtttt caagcggagg gttgtatctg ttttgttttt cggcggtgat
     7441 actttccttt tttcaaattc tgttttgttg tgtaaaaaat cgcaaacaaa gcttgcaaat
     7501 ttgaggaaaa tcggctatac tgaatagtgg agtattgtat gagcgtgttc tgcatccggc
     7561 ggattttccg ggaggacaga acaggctccg ggaacagcag ttcgggacgg tgcagaaatg
     7621 cagcgccgga ctgccgtaga aaggggcatg acattgaaag cgattgacga agcacgccgg
     7681 ctgctcagct ctgaggcgtt tacagaaacc gcagtgctgc tgagcggcgg cacagatctg
     7741 gcagaagcat atcggacacg tctgctgcgt gccattgata catttacaca gcatttcggc
     7801 agcgaccggg agatcgccgt tttttctgcg ccgggacgga cagaactggg cggtaaccac
     7861 actgaccacc agcgtggtca tgtgctggcg gcaagcatca acctggatgt gatcgccgtt
     7921 gtggcacgcc gcaccgaccg gactatctgc gtgaagtccg agggctttcc ggcagatgtg
     7981 atctctctgg atgagctgga gccggtgaca gaccagaaac ccaccgcagc cgagcttatc
     8041 cgtggtgcag ctgcctggtt ccagcagtac ggatgtccgt tggataccgg ctttgatgcg
     8101 tataccacat cccaggtcat gccgggttcg gggctgtcct cttctgcggc atttgaggtg
     8161 ctgctgggga atatctgcaa cgccctgtac gccgacaacc ggttcactcc ggtggagctg
     8221 gcgcaaatgg gacagtatgc ggaaaacgtc tatttccata agccctgcgg tctgatggat
     8281 cagatggcgt cgtctgtggg cggcgcagtt gccatcgact ttgcgaatcc ggtaaagccg
     8341 gtggtgcatc cggtgaagtt cgacttttct tccaccggtt atgcgctgtg catcgtggac
     8401 accggcggga atcacgcaga cctcacagag gaatatgcgg cgatccccag ggaaatgggc
     8461 gctgtggcga agtatttcgg caaagatgtg ctttctgagg tgaagtcgga acaagtgctg
     8521 cgctcgattc ccgaactgcg aaaggcgtgc ggcgaccgtg cggtgctgcg tgccatgcac
     8581 ttctaccggg aggacggcag agcccagggc gaatcggatg ccctggagcg tggggacttc
     8641 gaggcgttcc tgcacctggt gcagaactcc ggcgaatcct cctactgcct gctgcaaaat
     8701 gtttacccat cctctgtgcc tgcggaacag ccggtctcca tcgccattgc agttggctcg
     8761 gcagtgctgg gcggacgtgg cgccatccgg gtacacggcg gcggtttcgg cggaacagtg
     8821 caggcatttg taccgtctgt gctgctgacc cagttccggg acggcatgga ggctgtgctg
     8881 ggcgagggct gctgccacgt gctgcagatt cgccccatcg gcggcatgac aattttgatc
     8941 tgacgaagat agaatttgac agaattcgaa aggaagcgta agaaaacatg ggaaaaattc
     9001 tggtcacagg cggtacgggc tttatcggca gccatacctg tgtggcactg accaacgccg
     9061 gctatccggt ggtggtcatg gacaatctga gcaattcaaa ggtcgagaca atcgctcgca
     9121 tcgagatgat taccggaaaa cacgtgcggt tttaccggac ggatatgcgg gacgaggcag
     9181 acctggaaca gatctttgag gacaacaaga tcgacgccgt gatacacttc gccggaatga
     9241 aggcagtggg cgaatcggtg gaaaagccgc tggaatacta cgagaacaac gtgagcggca
     9301 cactgacgct gctgcgtgtg atgcggaagt acggctgccg caccatgatc ttttcctcca
     9361 gcgccacggt ctacggcagc cagaacccgg tgccgtaccg ggaggagatg cccacctctg
     9421 cgaccaatcc ctatggctat accaaggtga tgatcgaaca gatcctgcgg gatgtctgtg
     9481 cggctgaccc caacttcacg gcggtggcgc tgcggtattt caaccccatc ggcgcacaca
     9541 tgagcggtct gctgggcgag aaccccaacg gcatccccaa caacctggtg ccgtatgtgg
     9601 cacaggtggc atccggcaag ctggatcatg tccaggtcta cggcgacgat tacgacaccc
     9661 cggacggcac aggtgtccgg gattacatcc acgttatgga tctggcagtg ggtcacgtgg
     9721 ctgcgctggc atacggtgca aaccatcacg gatttgaggc ggtgaacctg gggacaggtc
     9781 acggcagcag cgtgttggaa gtgatacacg cctatgagaa agcctgcggc agaaagattc
     9841 ccgtggagat caccgaccgc cgtgccggag acgttgccgt ttcttatgcg gatgtctcca
     9901 aggcgaagga actgctgggc tgggaggcat cggcagacct gcagcagatg tgcagtgaca
     9961 gctggcgctt tacgctgcaa aatccaaacg gcttataatc gcttgtgtcg ggaatctgcg
    10021 gaaacaggtt cccacagaaa ggaatacata tgacaggaag atttggaaat gcgatcgaca
    10081 attttctgat tatcatggtc gcagttgcca ttgtgctgct gctggtggtg atgctgggtt
    10141 cccggcagag tccgttccgg cagaaactca gcaagctcct ggtgcagaat cccaatgccg
    10201 cagaccggcg gcagccgctg gaattcaacc gggaatatta tatcacagga gagagaccta
    10261 tgcgtaaacc aaatcgtgga aagaatccgc cacgcactgt gatcgctgtg ctggtgcaga
    10321 aggtggacga acgccgccgc accatggatc ccagcggcac gatgaagttg tataacgagt
    10381 actacgagct gatcttcaag acgagaaagg gtgatgtcct gcacatcgtg accagcaaga
    10441 aggcgtacat cgagacaccg ttccaccagc agggacagct gacgttccag ggcgaccgtc
    10501 tgatccggtt ccagtatccg ggcggcgagg tcaacgagga tcaggacgct ttcaccagcg
    10561 ctatgccggc agcacgccgg gcgtaacagg tacgcagtac gttgagacaa aagaaaaggc
    10621 ataccgtttc cgagcgagac ggtatgcctt ttgtgcgtta tggaattttt acggaataat
    10681 catcaccggt gtgttgatgg ttacttcgtc gtagatcttt ttggcaacgt ccagcggcag
    10741 gttgatgcag ccgtgggagc cgttgttgat gtagatgtca ccgccgtatg cgctgcggtt
    10801 caggtcgtgc agaccaatgc cggtataggt gacaggcatc cagtagttga cccaggtctc
    10861 atagccctgt acagcgtagg tacccagctt tcggggcgat tctctggacc ataccttgta
    10921 tgcgcccggc ggtgtggcac gttccggatc ggattcctta ccggttacca cgtcggactc
    10981 caggaagagt tcgccgttca cataatacca cagatgctgg tggcagatgt ctacttcaat
    11041 gtaggtgttt ccggtgtatt tcacgccggc gttggagttc atgcggaatc cttctacctt
    11101 atagaccggt tcgatggtga ccgcatcgcc tgccttgatg taatccacca gcttttccac
    11161 cgtggcagtc acgttggttt tccagccgta gatgccgtcc acgccgatgg gcagggtaat
    11221 ttcgccgtcc tcggtcgcct tgaacttgaa gttgtagccg gtcaccaggg tatcgtactt
    11281 gtcggcgata ttttcctgta cccaggcagt cgccttgtcc gtgtctacgg tgatgtcgtc
    11341 gccgtccgtg gtgatccagt ccatgatggt ggcgtgatcc agcttttccg tgccgaccgg
    11401 atcgaagatc tcttcccggt ctgtcatgtc ataggtaatt tccacggcgc cgatcttgtt
    11461 gtacagctcc agagtgggtt tcagactctc tgcggtgacg gctgcctttt tgtagcagtc
    11521 gctttccgcc atgttgatgg tgttgttgcc ctcacgcatc tgcgcaatcg tgtagtcgga
    11581 caatttctgg gtgtccacca tgttgccgtc atcttccggc tggatggtga aagagccgtc
    11641 ctcgttctgg acaacctttg cgtccgtagg cggcacattg ccccagtcgt aggcagcaat
    11701 caggctgacc agtgcgtcct cggaatagga gtcctggact tttacggtgt actccgtctt
    11761 ggagaatagc tttttgaacc atgcggcgtg gctttcgtcc gccggcgctg ccagggcgtt
    11821 gtccggcagg gtgacggtgc agccaaagga agaacccttg aaggtgatgg ggtcgccgct
    11881 cttcgggatg aaggtcagcc ccatgtcctg ggctgtgctg aggacagcat tctgggcatc
    11941 ttcaacgttc atgccgccga tgtccacgcc gttcacatag gttttcggca ggaactttcc
    12001 gtgatagatc gccagcccgg caatgtagct cagcagcagg actgccagca ctgcgatgcc
    12061 ggcaatgacg gcgatacggc cgccgtgacg ctcttcccgg acttcttcat agtgccgggg
    12121 cggaacaggg cgggggcgct ctgccacagc cacattgctc tgccggggct gctgccgccg
    12181 ctggggaatt tcacgggatt cgttgttccg gggctgtccg gagcggctgg gtggcgtctg
    12241 gggttcgctg cggtgtgagg aagaaacggc atcccggatc tgccgggctt tttcggagcg
    12301 gctgcgctct gcggcggaat tgccggatgc gctgcggtac gcctgtccgg aaaagcgctg
    12361 ggaagtgctt ttcggcgttt cgggatgaaa ctcggatgcc tcttttgccg ccagggatgc
    12421 cttgatcttt tcccgcatct cgtccttggt gagatgcctg gggtcgtttc cggtgccgcc
    12481 gtgggaattc tggtctctgt tgttcactgt tatctgtcct ttcttccatt ttacgagggc
    12541 tgcccccgaa gagcagcgat ccccgaactt tgccggacgc ctctcgttcc atgcagcgtt
    12601 gatgaacgtc tcttgatacc ggcaaattcg aaaacagcag aacctttttt attggaaaaa
    12661 taaaatgtgt tgcaattcag aaaggcggca gctttgcagc gcatcaagcg ccaaagcacg
    12721 atcttgccgc cggcatattt tcatacctta tttattatac ccactgtatt tgggtttgtc
    12781 aacacttttc gacatgaaat ataaattgta aacacctttt accgatttgt ttccaaatcg
    12841 aagtgggaat ttgcacggat tgttgtagta atgtatgcag aaaaacagcc atatattccg
    12901 tgaaaaaagc tgattaaaat aaacaaaacg ataaaatgta ttgtgcgtta tgcacagaaa
    12961 aattcttggg aaatttggtg aaaaagtaca ttgacataga ccggaaaaag gtgtaaaata
    13021 ggggcgaaat ctgagcttgg acaccggctg ttccgcactt tctgtgcggt gcagcgcagc
    13081 gtgaacaccg gaaaaagaaa agcggtacaa aagcgtaccg tatcgtttcg gcacatgggg
    13141 aatattggaa aagccaatgt gtccgtgcgg cgtttgtcta tgccggaaaa cgggaggaat
    13201 cattatcatg aagagtaaga aaattggggc agggctgctg gcatttctga tggcagcgac
    13261 cacagcggca gcgagtgcgc agacggcgtt tgccgcagat ctggggacgg tgacggacaa
    13321 tgttccggca atcacggcgg cgagtgcgtc tgtgtcagtt ctcacagcca gcggcgacct
    13381 ggaggcggcg tatgcagaat ggggcgctgt ctccggtgca acgggctata acgtctatat
    13441 caagtccgca ggcggcagct atacccagct ggacaccatg ctggtgcgcc agtatccgga
    13501 tcgtttccgg gcagacgctg tgggtctgaa agccggcagc tataccatga agatcgtgcc
    13561 ggtgatcggc ggcaaggaag atgcctccaa ggcggcggag acctcggagt tgaacgtaga
    13621 ggcgcatgac agaagcggtt ttggctttgt aaacggtact tcttccggcg catacaacga
    13681 ggacggcacg ctgaaagcgg atgctatcgt ggtatacgtc accgatgcca acaaggacac
    13741 tgtgactgcc agcatcgact ccaccggcaa gggtgcggca gatgtgaccg gcgtgcagaa
    13801 catcatcacg gcatacaaga agaacaagga gaagcgtccg ctgtgcctgc gctttatcgg
    13861 caatatcacg gacccggcag atatgcccaa gggtgatctg atgatcgaca ccgccaaggc
    13921 tggcatcacc atcgagggca tcggcacaga caccgtgttc aacggctttg gtctggtcat
    13981 gaagaactgc tccaacgtgg aagtgcgcaa catcggcttt atgaactgcg acagcagcga
    14041 gggcgatgac tgcggcctgc agcagggcaa cgaccatatc tgggtgcata actgcgactt
    14101 cttctatggc cacgcaggtt cagatgcaga ccaggtaaag ggcgacggcg ctctggacac
    14161 caagacctcc acctatgtca cacattccta taaccatttc tgggacaacg gcaagtgcaa
    14221 cctccagggc atgaagtccg agaaggagac caactatgtg acctatcacc acaactggta
    14281 cgatcactct gactcccgtc acccgagaat ccgcacctgc tccgtccaca gctataacaa
    14341 ctacttcgac ggcaacgcca agtacggcat cggcgtgacc atgggtgcat ctgcctttgc
    14401 ggagaacaac tacttccgca actgcaagaa ccccatgatg agttccggac agggtacaga
    14461 cgcactgggc gaaggtacat tctccggcga gaccggcggc atcatcaagg cgtgcggcaa
    14521 ctacatcgag ggtgcatcca gctatatccc gtattcccag gacagcacct ctttcgatgc
    14581 ctatgaagtc tccagcccga cggaaaaagt tccggacagc gtcaagaccg tttccggcgg
    14641 cacaggctat aacaacttcg ataccgattc cagcatcatg tacagctacc aggctgacga
    14701 cgcaaaggat gtgccggcga tcgtcacagc caaggcaggt cgtgtccagg gcggcgactt
    14761 ccagtggaag ttcaacaatt ccgtggacga tgccagctat gcagtcaacc agccgctgaa
    14821 agatgcgctg atgaattata cgcccactgt cgttgcgatc ggcagcggct ttacggacac
    14881 caccaccgac ccggtcgtga ccacagagac caccaagcag accacggtga caacgacaac
    14941 caccaccgtc agcgtgtccc aggacacctc tgcaacggca acgactgtga ccaccagaga
    15001 taccacaccc accacaccgg atgtgccggt ggaaggcgac attttctgct cgccggacgg
    15061 caagggcagc ggtacttcgg aaaaggatcc ggcatcggtg accgacgcca tctccaagct
    15121 gaccccaggt cacaccatct acctgctggg cggcacgtat aacttctcgg agatgatcct
    15181 cattgacgac aagaacaacg gtacggcaga tgccatgaag accatcaagc cctataacgg
    15241 cgcagacgtt accttcgact tctccggaca gggtgacgca gacgcatcca agcgtggcat
    15301 cgtgctggac ggcgattact ggcacttcta cggctttgag atcacaaagg ctgccgataa
    15361 cggtatgctt ctctccggca gcaacaacag gctggagcgc atggtattca acgacaacca
    15421 ggataccggt ttacagctgt cccggtataa aaccagtgca gctaccattg cagactggcc
    15481 cagcaacaac ttgattctga actgcacctc caagaacaac tgcgacaacg ccaccatgga
    15541 aaatgcggac ggcttcgcag caaagctgac ctgcggcgag ggcaacgtat ttgacggctg
    15601 tatgtcctac aacaacagcg acgacggctg ggatctgttc gcaaagtctg agaccggtcc
    15661 gatcggcgtt gttaccatcc agaactgcgt ggcattccgc aacggctata ccgagttcgg
    15721 cgagggctac ggcaaatgtg acggcaatgg tttcaagctg ggcggttccg gtgtcggctc
    15781 tgcccatgtg gtcaagaact gcctggcgtt cgagaatctg aactgcggct tcaccgacaa
    15841 caacaacccg aaactggaca gtctgaccaa ctgcaccgcc tataacaacg gtgtcggctc
    15901 taacggcaag cccaatgtca gctgctatcg ctgcacagac gacggcgcag acttctccaa
    15961 cctcatctcc tactacagca aggagaatct caccggcaac ctgggcgtaa gcggtctgaa
    16021 gatcaccaac gacaagatgg tcggcacgat ccagaacacc atctactata actccggtta
    16081 ttaccaggtc ggcgccaaga ctgccatcag caacggcgat aagctgggaa caaagggcgc
    16141 agccatttcg gacgatgact tcattctggt gaccgcaccg gaaatgggaa cggatttcga
    16201 caccgcatgg cgcaactcca acggttctat caatacccac ggctttatgc aggtcaagga
    16261 aaacagcagc ctgtacagca ttctgggcgc tgccttccag gatatcgaca caccgcagcc
    16321 gccgatcaca acgacaacca ccagtgcaac gactgctaca acggaaactg ccacaaagac
    16381 agagaccaat ccggcacagt ccagtgagac tgcttctgca acagagacaa agccggcaga
    16441 gtccagcgtg accgcatctg caacggagac caagccggca gaatccagcg agaccgcaac
    16501 ggcaacagag acaaaaccgg cagagtccag tgcgaccgca acggcaacgg aaaccaagcc
    16561 gggcgaaagc actacggaaa gtgcaacaga gtccaagccc acagagaccc agaccaccgc
    16621 aagcgagatc gcaacggaac tgctctacgg ggatgtgaac ctggacggca gagtggatat
    16681 cacggacgct gtcctcatca acaaggcgat cgccggcgca gtggaactga gcgcaaacca
    16741 gcgccacaac agcgatgtct atgcagacgg cgaactgacc acaagcgacg caacgacact
    16801 gctccagttc ctggtaagca tgatcaagat tctgcctgtg acggaataaa tgaaaacagg
    16861 ctctgaaaac tgaatcagaa gaaaggcggc tgtatttcgg tacagccgct tttttcgaca
    16921 aaatatgact ccaaaaattt tttgaatatg ccaaaagcaa gactgatttt ttatgcaatc
    16981 tgccaaaaaa acagcttttt ccgtttagta cagcgcaaag atcggtattg ctcttgcaat
    17041 ttttgccggg atgtgctata ctatagaaag caatgctgtt gatttgtgaa tgacggcatc
    17101 tgcactattt ctatccctgt aacttcctgc gtgactgcgc agtgccgttt tcgggcgata
    17161 aatcttggat gaggtgaacc aaattggcac aactgaaaaa acccacaatc aaaaaccccg
    17221 ctctggtatc cgcaatggca gagttcaagg caaagcagaa tccgcagact gaaaaagaaa
    17281 tgctggacgc aattgctgag gcatccttta ttgccccgat cgctttgcag aaacagctgg
    17341 atgaggtaga gccggacgaa aacggccagc gccagatgca ggcgtctctc atggcagttt
    17401 ccaacaagga cggctcgaag tttttcccgg cgttcacgga ctggctggaa tttctgaagt
    17461 ggaagaacga tccggaggca gacaccatgg tcatcacatt tgaccagtac tgcggcatcc
    17521 tgctgaaaca gggcgtggat atcaacggta tcgttatcaa tccgtcagag accaacatcg
    17581 tgatccgcaa ggaaaagatg gcagagatga agggcgtgtc tgttcccgca gatgcaccga
    17641 agaatcaccg catcatgccg ctgtttggca ccgagaagat caccaactct gaggtcatca
    17701 tcgccgcaga ccgtctgcgc cgggagaaca acgaggacgc aaagaacaat ctgttcaacg
    17761 taatgcgcaa ggctcgtttt gtcgctccgg ttctgatgga gatgccgaag gaagtcaagg
    17821 ctggcgagaa gatcaacgcc caggcggaat tcattatgct gaaccacgac ggcggaaagt
    17881 tcatgccgct gttcaccagt ttgccggagc tgcagaagtg gacaggcgca ccggactgca
    17941 aggcagttcc catgagcatg gcgaactata ccgcaatgct gtccgacccg aagagtacgg
    18001 ctgccggcat cgtgatcgac ccgttctcca tgggtctggc attcagcaag gaacaggcgc
    18061 tgaacatcca gccccgtctg gaactgcgtg acatcgaggg cgttccggag ggaatgcagg
    18121 aggcactgag agaccacctg gcaaccctgc cggcagtcaa gaaggcgtac ctggacggca
    18181 tcaaggcaaa cggcgcagac ggctatgtga tcgtcatgga tctggaaaag gatgcagata
    18241 tcccgaccat cgcagacagc accgcacaga tcgcaaagca gtacggttcc tgtgtcgttg
    18301 caccggtaga ttctccgctg ggcaaaaagg tgctggaacg gagagagacc ttctacgagg
    18361 cgtaagggaa atatagcgta agggtaagaa caaggaaaaa atttgaagga aagaaaggat
    18421 gattcaaatg aaaagaaaag cagtgattct ggcaggcgct ctggcttgtg cggcggttgt
    18481 ctctgcggca atgcccatgc agtgcagtgc ggcaggcgca ctgccggctg gcaaagcgtt
    18541 tatcgcagtc aacggagaag tactcgaacc ccaggtgacc atccagggag acggacagta
    18601 taccgtttcg tgtgatacgg cggcgctccg ggaaagcgat acggtgcagc tgtgggtgga
    18661 aaacattgac ccgattccgc tgggaacaaa gggttcaaca agcatgactg tggattccat
    18721 ccagttcaac ggcagtgccg cagcggtgga cggcgatatc atcgaagcat ccaagacgga
    18781 cttcggctat tccaaggatg ttttctgtgc gatgatctat cgtttcacac cgatgcagtc
    18841 cgatgcggca accaatcacc tggatgtcac ctttacggtg tcgaatctga tcgacccggc
    18901 gacctatgtg ccgagcaatg tctccacggt tgttgccaca gggacgacca ctatcgaatc
    18961 cggcagcagt ttcggtgcat ccaacggcgg cggcagtgtg actggcggca gtgtgaaaac
    19021 cagtaccagc ggctctccga aaacgggaga tgccggcgtg tacgggctga tggctgtact
    19081 ggttgtcagc ggcggcgcag cattcctggc aaagaagaag gcttaacata atatatcaca
    19141 gtatatagaa aatcacccct gttctgcggc aagtgaaatg ccgtgagagc aggggtgttt
    19201 ttgtttttgg atttatgctt gaaaaaactg ccggaatgcc gtgggcaggg aaatctctgc
    19261 ggcaatttct gcggcggttt tccagacgaa ctgctcgtcc tgtctgccgc aggtgagata
    19321 gacgccgtac agctcccatt ccacgtgggt gaagatgtgc tttcgctctg ccgtctgggt
    19381 cagatccaac ggcttgcact gccaccggct cacctgggcg attgcctctt ccgtggtgca
    19441 gatgccggac acattggggt attcccacag cccgtggagc agaccttttg cggtgcgttt
    19501 ccggacggcg tattttccgt cgcagcaaag cacaaacacg gtgtattgtt ccttgcgccg
    19561 gggctttttc tcggtgcgct gtgggagacg catggtgtcg ccgtactgtt ttcccagaca
    19621 aagttccgcc aggggacaga cctcgcaccg gggctgtccg ttgggcagac agaccgtagc
    19681 gcccagctcc atcagcgcct gtgtcatcgt gccgcactgt ccgggaatgt tccggtacac
    19741 ctgttccagc gaccgagtga cggctgcctt ttgttccggg cggtcgatct cgcagaaact
    19801 gtcctggatg cgtgccgcca cacgcagcac attgccgtcc accgccggcg tgggcaggtc
    19861 atagcagatg gagcagactg caccggcggt gtagtcgcct acgccgggca gctgccggac
    19921 ttcttcccac gtttccggga atgcgccgcc gtattgttcc acaatgacct gcgccgcctt
    19981 gcgcaagttg gcagcccggc tgtaatagcc cagaccttcc cagcacttgt tcacctggtc
    20041 ctgttcggcg tttgccagag caggaatgtc ggggaaggtc tccagaaagc gctggtaata
    20101 gcccttgact gcctcaaccc gtgtctgctg gagcatgatc tccgacagcc atacccggta
    20161 gggctgccgg ttctttcgcc agggcaggtc tctggcgtga ccgggatacc acgacaaaag
    20221 cggctgtgtc agctgtgcca tgacctgttc ctgcatggct ttccaccggc gcatccgggc
    20281 gatgaccttt tcatacaccg ggagcatatg aggttctttg cagatctccg acagcttttc
    20341 cacggggatc cagtccacgg cggtgttttc gtccggggcg atgcgcagcg tctctctggt
    20401 atctgcaatc agcccgtagg tcacgttgta gtgctggtgt tccggaacgg gtgtgccgtt
    20461 tttctggtgc gccctgaccg ggagaatgtc cagggacaga acagcgccgg tgaggggata
    20521 gggctttcgg atgccggtct cttcctttgc ctcccggacg gcagtccaga gaaaatcgtt
    20581 ggaaccgtct gcgtgaccgc ccgtccaggc gaaggagtcg tagatgcggt gatagaccat
    20641 cagcaccttt tccagcctgg tgtccatgac gaatccggaa caggtaaagt ggctttctgc
    20701 gtggctgcgc caaagcagct gtgtgccgta ttgttccgcc gcaaggagta tctcgttccg
    20761 gtcacggcgt tcctgttcgg tttcgggaac ataggcggcg agacgggctt tccagtcggt
    20821 cacatcctgt tcctgcatga catttctcat gttgtctgtc ctttctgtga gagcctgttt
    20881 cgaccgtcag atacgaaaca ggctcttgca attgtttttc atttttccgg cagcaaaatc
    20941 gtgatctccg tgccgtgtcc cagttcgctg cgcagggaga tggtgccgtt gtggcagtct
    21001 acgatgtgct tgacgatgga cagccccagc ccggtgccgc cacgctgttt cgaccggctt
    21061 ttgtccaccc ggtagaaccg ttcgaagatg cggctctgcg cctggagcgg aatgccgatg
    21121 ccgttgtccg caatgacgat ctttgcctgt gtacccacgt gttcgatggt gatggagact
    21181 gtgccgccgg gattgttgta cttgatggcg ttgtcgatga ggttgatgaa aagctcccgc
    21241 atatagcctt cgttgctgtg cagcaggaga catctgccct ccagatgtac ctgtatcctg
    21301 ttccgctgga tctggggttc cagaatttgc agggcgtttc cggcagccgc cgccaggtct
    21361 accggcgtgt tcagcatctc ggtgtgttcc tcgatctccg agnnnnnnnn nnatgtcgtt
    21421 gatgagaaag agcaggcgtt cgctttcccg ttcgatcatg gcgccgtatt tcgccacgtc
    21481 gtcctctttg gtaatcatgc cgctgccgaa catctcgccg aatcccttga tggtggtcag
    21541 cggcgttttc agctcgtggg agacgtttgc ggtgaattcc tggcgcatct tttccgcctt
    21601 gacgttatag gtcacgtcca ccagcagcac gatcgtcccg aagatgccgc ccatttccac
    21661 acgggtgatg tacgcccgga tgtagctgcc gtcgatcttc agcagctggc gcacggtgca
    21721 gcccttgtga agatcatcgt cagccggcgg catatcgtgc tgcggctctg ttttggactt
    21781 ccgcagcgca tccagaaatg cctggttgtc ggtgaggatg aagatggatt tgttgtccca
    21841 gttttccttt tcgatgctga gcagtgccgt tgcggtgcgg ttcgccagct ggatcttgtc
    21901 agaatcgtcc aggatgagga tgccctctac catatgctcc tggatgagtc cgatgatgtc
    21961 ccgttcgtgg tacagttcct tctgcatccg gctcatctcc gtctggagct ttgcctgggc
    22021 tttctggtct tttttgtgct ggtgctgtgc ctgctgtttc tggtgcaggt acagaaagat
    22081 aaataccgtc agcagcaccg ctgtcactgc agcgaatgcc catccgatcc attccatgat
    22141 tagcctccga tcttatagcc gtaaccccgt actgtgtgaa tgagattgga acatcctgcg
    22201 gcatccagct tttgccggag cgttttcacg tgtgcatcca cggtgcgtgt ctcgccctca
    22261 taggagaatc cccagacctg ttccaaaagg cggtcccggg aaatggcagt gccacggttg
    22321 cgcaccagat aggacagcag ttcgaattcc ttataggtga ggacgatctc tgtttccaga
    22381 tagaccaccg tccgctgatc caggtcgatg gtcagatcgt gggcagacag cacgtggctc
    22441 tccgctttct gtaccgtgtg gctgcgccgc agcaccgcct tgatccgtga cagcagttcc
    22501 atgatgccga acggctttgt gatgtagtcg tctgcgccgt tttccaaccc ggtcaccttg
    22561 tcgatctcgc tggacttcgc cgacagcata atgaccggta tctctgcggt agcgtcgttg
    22621 cccttgagcc gctttaagat ggagatgccg tcctcatcgg ggagcatgat gtccagcagg
    22681 atcagcgccg gcttgtcgtc ccggagccgg tgaaaaaagc tttttccgtc cgggaatccc
    22741 tcggcggtat agccgccgga ctgcaaagcg caggtgataa gctcccggat tcccgtatca
    22801 tcctctacac aaaatatatt tgccaactta catttcctct ttctttccct gtcggatctc
    22861 ccggcgcatt gccgaaacag caacttcgat ggcgtgcgtc atgtcgccgg cggcgtgtgc
    22921 attgtcccgc accaccgcct ggcgctcctg gttccacagc atttgcagga tagtggcaca
    22981 cttcacccgg cgtgtcgccg ccacgatgaa cagcgccgcc gattccatct ccgatgccag
    23041 cgctccggcg gctttccacg cctgccagtt ctggagcagg gtctgtgcgg caggcatggt
    23101 ctccggggaa tgctgcccgt agaaagagtc cttgcactgc accacgccgg tacaataatc
    23161 atatgcaagc tcctgtgcgg cgcagaccag atgctgtgtc agaccgaagt ccgggacagc
    23221 cggaaatgcc aggggaacgt attcctgcga tgttccctcc atgcggatag caccggtggg
    23281 gatgatgagc gttcccggaa tcagttccgg ctgcattccg ccgcaggtgc cgatgcgcag
    23341 aaaggtgtgt gcgcccagcg ccgccaattc ttccatggcg atggcagcag acgggccgcc
    23401 gatgccggtg gaggtgacgc tcactttcgt gccgtccagt gtgccggtcc atgtggtgaa
    23461 ctcccggttc tgcgccgtct ttacgggatg ttccagataa gaggcgatcc actcggtgcg
    23521 tccggggtcg ccggggagaa agacatactt tccgatgtcc tccgctgtgc attgaatgtg
    23581 aaactgacgt tctgacataa gcggttcctt tctgcgtgtg aaacaggttc tggcgggcgg
    23641 gggttgtctt tgttgtgcaa atgtgttccc ttgaaaaggc gcccataggc ttgactgagg
    23701 cgtaacacac caacgggcga acactgtttg catctacaga agtatcacaa aacggaaacc
    23761 gtagatgtgc agaatccctg tctcaccgta ggggcgaaca tcgttcgccc gggtcgactc
    23821 ctctgccctg ccgggcatct cctctaaaag aggagacata ttaaaaagcc ttccttgaaa
    23881 agggaggtgg cacgaagtgc cagagggatt atctacattt acacttcttg acaaatttcc
    23941 agaattctgt tatgatatac ccatgaacaa cattttcagc accaccgcct aaaatctgtg
    24001 cggtggcatc ttgattctgg aggaaagatc atgcagcaat accgcaactg gcagccgcag
    24061 gagccggagc atcattatga tatgagagcg ctggtctttg gcgtagtgtc gtttctgttc
    24121 tgtcagctgc cggtcctgtc cattgtgacg ggcatcatcg ccctggtcta tgcggcaagg
    24181 gcgaaaaaac ggcagcgctg ttacagccac ggcatggtaa cggcaggcat gatattgggt
    24241 attatcggga tctgtatggg tgtattgtcc agtatttact ggggctttaa ttttctgcgg
    24301 attctctgga tgctttcaga gaacaaccgg atggtgcctg cgccgtccaa cccggagttt
    24361 ttcaacggtc cgggaacgcc ggcggcattt ttcccgttat tctgagtcca ggtaatccag
    24421 gtcggaatct tctgtttcag cgccgtgatc gggctgcgtc tcaatgcctt ccgggtgtgc
    24481 atcttccggg agcggcttta cgacagcaaa cccgctctcg gcgttttctt ctgcctgttc
    24541 ccggcagacc ggatctacgt ggaatgtgac cgcctgctta cggtttgcga tctcgttgac
    24601 acgctctgtg ccgccgtatt ccccgtcgat cgtccaggac accggctcgt cgtccagcgc
    24661 agtgactttc agatgtgagg tgcggaaata gtggagatac tgtttcgcca gccccagctg
    24721 gatcttggag aacgccatga ccagcttgtg gaattccacc aggttggagg gcttgtggat
    24781 cagtgtgacc tcgaacaagc cgtcgtccca ccgcacatcg gacagcgcca ggaacttcgc
    24841 cacagaggaa gagttggtta ccatgccgta gaggaactcg tcctcgatca cgccgccgtc
    24901 gtactcaatg cgcatccggt acgcccggat cttcggcagc ttggaaatgg cgttgagcag
    24961 gtaagcagag tgtcccagca ggttcttgaa ctgctgcggc gtttcatagg agatctccgt
    25021 gaatgcgccg aatgcagcta cataggtgaa gtatttgtcg ttgaagctgc ccacatcgca
    25081 gcggagatct tcgccgtgga tgatgcagtc tacggctgcc tccggttctt tgggaatgcc
    25141 cagtccacgg gcaaaatcgt tggttgagcc gaagggaata tagccgatgg ggcattgggt
    25201 ctcgctttgc atacagccct ggatgacttc gttcagtgtg ccgtctccgc cggagcagac
    25261 caggatgtca taacagccgg catcacacgc ctgttttgcg cacacagtgg catcgccacg
    25321 atcttgtgt
//
LOCUS       DAWENO010000008        24390 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_842_length_24390_cov_13.240189, whole genome shotgun sequence.
ACCESSION   DAWENO010000008 DAWENO010000000
VERSION     DAWENO010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24390)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_842_length_24390_cov_13.240189"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    23164..23173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5530 a         6511 c         6916 g         5423 t
ORIGIN      
        1 acagccccac acctccgcag gacgtgtgcc gaccttcagc ggctatcgga tgtatattga
       61 agagatcagc gccggcggtt cgctcccgga ggaagagcgg gagcggctgg acgagatgct
      121 gggagatgaa tcccagctga cagaggatct gattttacag agcgccacga cggcactctc
      181 tcagattaca aagtgcgcaa cggtggtctc caacttcgca cccaaatatt ccgtgatctc
      241 caaggtggat gtgatcccga ccggcaagcg gatgtacgtg gtgctgctta tcacatcgga
      301 tggcaagatc aagaaccgtg cctgccgctt acagtttgat ctgacccatg aacacatgga
      361 tttcttcaaa cactttatgg aagagaacct ggagggcgtt tcggtctcgg atctgtccga
      421 agaaaagctg gatcagatgg tgaccgccat gggaacctac atgatgaccc tcagcccgct
      481 ggtacagggc atctgcgaaa tgtccaagga cttgcagcag aatgagctgt atgtcaccgg
      541 agagcagaac ctgttctcct gcgacgacct ggataaggca gaggtggcaa agttcatcct
      601 gcataaacag gagctgtgcg gactgctgga cgactcgttc cagggcatca aggtgatgtt
      661 cggagaagag ggcaacgatt ttgtcatcgg caactccagt atgatcgttt ccaagtatca
      721 gaaaggtgac cacaccgctg gctcgctggg cgtgatcgga ccggttcggc tgaattacgc
      781 caaggtgata ccttatatag aatattttac cgaaaagatc tcacatctga tctcggagga
      841 ggacaaaacg ccgccggaat tagagatgat cgaaaaacca gacaacgatt cggaacacaa
      901 gccggaacaa tagaaaggac gtgtttttga tggcagacga aaaggaaacc aaaaagccgg
      961 aaactgctgc ggaaaagccg aatgcttacg ataaatttat tgcaatagtg caaaagccag
     1021 aggcagagag aacaccggaa gagactaagt ttgtaaatga ccttcttgca ctcttccgta
     1081 acaacgacgc aaagacagag aaagcgccgg aagaaactgc tgcaccggag accaaggcag
     1141 aagaaaccaa ggacgacaaa aagaagaaga aaaaagactt gaaggatgca ctggcgcaga
     1201 aagaggcaga gctggcagat cagaaagacc agcacctgcg gctcatggct gagtatcaga
     1261 actaccgcaa ccgcaccacc gaggaaaaga agaagatcta cggggatgca aagatggatt
     1321 gcgtaaagga actgctgaat gtcatggaca cctttgaacg tgcgctggag gcaccttgta
     1381 ccgatgagaa ttacaaaaag ggcgtggaac tgaccttcgc acagatccag aaagctttcg
     1441 aaaagctggg cgtgacagag atcgccgcat tgggcgagac tttcgacccc aatctccaca
     1501 atgccatcaa gcaggtggac gacagcgagt atgagagcga caaggtctgc gaggtgttcc
     1561 agaagggata cctgctgggt gaccgcctca tccgtccggc aatggttgcc gtttctgtgt
     1621 aggcaggagg caggacagtg aagaaacaca cagcgtctct catgacggcg gcgatgctgc
     1681 tgacactggc gtggatgacc ggatgtgatt ccggaaagac cgcagaatcc agcaaggcag
     1741 ccaccaccac ggcactggaa accaccgcag aggtcgtgac cgatgcggca gaaactacgg
     1801 cggcagaaga aagctccgcc gcagatgcac agcgctttga caactatgca gattttgccg
     1861 cagcgatggc agaacagcat ccggaactga cactgtatac gccgccggaa agcgtgcagc
     1921 agcagtggga atggaagtcc atcatgctgg gacagaccag ctatcagtac gagatgtaca
     1981 gcgcagaaag acaggcaacg gtgaatgtcc tcattgatat gcagccgtcc tttaccgatg
     2041 cgcaggagat cgtggatacc ctgacaagca tgggtgttac ggcaaaactg atcgacgatg
     2101 gctgctgttt gtttgagcag gacggtgacg gaatgtatgc cctgtacggc atcaccggtg
     2161 acgccggcga gaactttgcc gctacgctgg aatatgatga cggcacaacg gcaacggaag
     2221 atgagctgaa agcactgcgc agcgaattct ggttataaaa atccctgtcc gaaaggacag
     2281 ttgaaataaa tcatatttat tttgaaaaat ctgcccgttt ttccgcaggg gaaacggaga
     2341 tacagaaagg aagaatttat catgggaaag attattggta ttgacttggg tacaacaaac
     2401 tcttgtgttg ccgttatgga aggcggcaac gcagtcgtaa tcccgaacgc agagggtgca
     2461 agaaccaccc cgtctgtagt tggtttctcc aagaccggcg agcgtctggt gggtcagacc
     2521 gcaaagcgtc aggcaattgc aaacccggag cgcacgatct cttcgatcaa gcgtcacatg
     2581 ggttctgatt ataaggtaga tattgacggc aagaagtata caccgcagga gatctccgca
     2641 atgatcctcc agaagttaaa gaccgatgca gaggcatatc tgggtcagcc ggtaactgag
     2701 gcagttatca ccgttccggc atacttcacc gactctcagc gtcaggcaac caaggacgca
     2761 ggacagatcg caggtctgac cgtaaagcgt atcatcaacg agccgaccgc agctgcgctt
     2821 tcttatggca ttgataagga agacgaccag agagttatgg tatacgacct gggcggcggt
     2881 acgttcgatg tttccatcat tgagatgggc gacggcgtac agcaggtact ggctaccgca
     2941 ggcaacaacc gcctgggcgg cgatgacttc gaccagagag ttatcaactg gatggttgaa
     3001 gagttcaaga agaccgaagg catcgacctt tctaccgata agatggctgt acagcgtctg
     3061 aaggatgccg cagaaaaggc aaagatcgag ttgtcctcca ccacaaccac aaacatcaac
     3121 attccgttta tcactgcgga tgcaaccggc gcaaagcacc tggatatgaa tctgactgtt
     3181 gccaagttca atgagctgac caaggatctg gtagacgcta ccatgggacc ggtacagcag
     3241 gcactgtccg acagcggcct gtctccgtct gatctgaaca agatcctgat ggtcggcggt
     3301 tcttccagaa tcccggctgt acaggaggct gttcgccaga agctgggcaa ggaaccgttc
     3361 aagggcatca acccggatga gtgcgtagca ctgggtgctg cataccaggg cggtgtcctg
     3421 ggcggcgacg tcaaggacgg tctgctgctg ctggatgtta caccgctgtc tctgggtctg
     3481 gaaaccatgg gcggtgtatg caccaagatc attgagagaa acaccaccat cccgaccaag
     3541 aagagccaga tcttctctac cgcagctgac aaccagtccg cagtagacat caacgtcctc
     3601 cagggtgagc gtgagttcgc aaaggacaac aagcagctgg gtaccttccg tctggacggc
     3661 atcgcaccgg ctccgagagg catcccgcag atcgaagtta ccttcgatat cgaccagaac
     3721 ggtatcgttc acgtaactgc aaaggatctg ggtaccggca aggagcagga tatcaagatc
     3781 actgcatcta ccaatatgtc caaggacgat atcgacaagg ctgtcaagga agcagaacag
     3841 tatgccgaag aggataaaaa gcgtcgtgaa gaggttgact ccaagaacaa cgcagagagc
     3901 ctgtgcttcc agtgcgagaa cgcactgaag gaattcggcg acaaggttcc ggcagatgaa
     3961 aagtctgcga tcgagagcaa gatcgctgat ctgagaagtg cagtcggcag caatgatgcc
     4021 gctgcgatca aggcaaagac cgacgatctc cagcaggcat tctatgcact gtcctccaag
     4081 atgtatcagc agaacggcgg tcagccgggc gcagacccga acatgggcgg cgcaggtttc
     4141 ggcggtgctg atccgaatat gggcggcaat ccgaacggcg gcgcaaacga tgacggcgtc
     4201 gtagacgcag acttcaagga agtttaagaa tgaaacgcag cggcgtggaa cgtgacgtac
     4261 cgctgtgaat agaacggcgg tgacggggca ggtttcggct tgtcccggca tcgttgtata
     4321 tgggagtgaa agatattccc atgatccata cagacaggaa cagctgctat gcgtctgttc
     4381 cagggaaagg agccagcaga catggcagaa aaacgggatt attacgaagt gctggggctg
     4441 aaaaagggtg cttctgagga cgagatcaaa aaggcgttca agaccatggc ccggaagtat
     4501 caccccgatc tgcacccgga cgataaagaa gcagcggaaa agttcaagga agtcaacgag
     4561 gcatacgaag tgctttccga ctcggaaaag cgtgccagat atgaccagtt cggtcatgcc
     4621 ggcgtagacc ccaactatgg cggcggcgct ggtgcaggcg gattcggcgg ctttggcgga
     4681 ttcggtgatg taggcgacat tttcgacagc atctttggcg gcggcggatt cggcggcttt
     4741 ggcggcagct ccagatcggc gtccaatgcc aatgcgcccc ggcgtggcgg cgacgtcaac
     4801 agcaatgtga ccattgattt tatggaggcg tgcaagggcg tcaagaagaa aatgcgcatc
     4861 acccatatgg tgaagtgtga gcagtgcggc ggcacaggcg cagatgccaa gaccaccagc
     4921 accacttgta ccgagtgtca cggtcagggt tctgtccgtg tgaaccagcg gacaccgttt
     4981 ggcgtgatct ccaccacaaa ggtatgctca cgctgcggcg gtaagggtaa gatcgtcacc
     5041 aatccgtgca gcaagtgccg tggcagcgga cgctatcgct ctaccaacga cattgagctg
     5101 aaagtgccgg caggcattga caacggacag gtgctgcgtg tcagcggtca gggcgatgcc
     5161 ggtctgaacg gcggtccgaa cggcgatcta aatgtatctg tcaatgtacg gccgcatgaa
     5221 ctgttcaccc ggaacggcgt ggacatctac tgcgagatcc ctatcaccta tgcacaggct
     5281 gttctgggcg ataaggtgac agtacctacc atcaagggca aggtggaata tgagatcccc
     5341 gaaggcacac agagcggcac gaagttccgc ctgcgtggtg agggtgtaaa gtatctgggc
     5401 agagaatcct acggcgatca gtatgtgacc gttgtggtag aagtgccgag aaagctgaca
     5461 cgccagcaga aagagctgct gcaaaagttt gacgacagcc tggaggaaaa gaaccaggca
     5521 aagcggcaga gcttttttga aaagttaaaa cggcgatttg agcagcagta aacggctgca
     5581 tgaaaaagcc tcatccgatg cggaaatgca cctggatgag gcttttttta tcggctactg
     5641 tcgtcgttct cgtccttctg ggagtcgtca tcgccgccca gaatgtcacc ggcagcatcg
     5701 ccggcatcct ttaccgcatc gcctgcggca tctcctgcgt cacggactgc gtctccggca
     5761 tcggtcacga tgtcacggac accgtcctct gcatcgctga caatgttgcc gccgtcgtcc
     5821 ttgctgtcat ctttgctgtc gtccttgtca tcccggctgc tgtcgtctgc ggcagagctg
     5881 ctgacagaat cgtttatgga gctgtcgtcc ttgctgccgc agccggtgaa caggctgccc
     5941 atcagcagca gtgccatcgg cagcgccaga tagtgcttga ttttcatggt ttcacctcat
     6001 ttttcagaga gcatatcggc gtggatgcgc ccatacactc cgaagattgc agaagcatcg
     6061 ctttgcaaga ttagtgtttc ccacaactgc ggagatattc cctttccaca tttccacggc
     6121 gttttttctg cgaaatttcg gggatttttc cggtatggcg caggttttgc cgaatatttc
     6181 cccaactttt caacacggtt ttccacacaa tgcggaaaac ttgttacaac ggggtgacga
     6241 aattcgaact ttccgcagcg ctccgctgtc gcatttcggc gtaatgtgta atgattgtaa
     6301 ccaattgcag aaaagcaccg gaaaaacagc agtttttgtt ttccacaagt tgtggaaaac
     6361 gtggtggaaa gatcgggaga actgtggaaa tacggagatc atccggaaag aacggcacaa
     6421 aaaaagacac ggcgaaccgt gtcttttccg gatatttgtt gtaagattaa gcagccttta
     6481 gtctttctgt acggcgtcgt acaggtcgct tttccggtga cctgtctctg cggcaacggt
     6541 tttgcacgcc tgggtcagtt tctgcccgtc tgccacatac tgccgcaccc gttctgccgc
     6601 ctcttccagg gtcagctgtg cctcctgctg cggtactgcg ccggcaacca ccagcacaaa
     6661 ctcgcctcgt ggcggtgtgt tttcgtagta ggcgatggcg tccgcaaagg tagtcagacg
     6721 cacctcctcg tggatcttgg tcagctcccg gcagagagac aggctccggt ctccgccaaa
     6781 gacagccgcc atgtccgcca gtgtgcggca gagcttgtgg ggtgcctcat agaagatcat
     6841 ggtgcgctgt tcctgccgca gctgttccag gtgtgcggtg cgctggttgc ggttcacgga
     6901 gagaaagccc tcaaaggcaa agcgtgcggt gtgctgtccg gagacggcaa gggcggcaat
     6961 ggctgccgac ggtcctggaa caacttccat cggcacgccc agcttgtgac agagctgtac
     7021 cagcggctcg cccgggtcgg agatgcaggg cattccggca tcggtgacaa cgccgcagga
     7081 ttcgccggca aggatgcggt cggcgatgct ctgcgccacc gtcagtccgc tgtgttcgtg
     7141 atagctgacg atgggcgtgt ggatgtcata atgggacagg agctttcggg tcacacgggt
     7201 atcctctgcc gccagaaagt ccacctcacc cagcagccgc agctgacgca tggtgatgtc
     7261 ctccaggttg ccgatgggtg tgccgatgat gtataatgta ccgctcatgt tggctctcgt
     7321 gtcctttctc agaaaaactg tttcatttcc agaatgtcgt ggatgtgtac ggtggcagtg
     7381 gcgcagatct ctttccagac cggcgcattt tccgggtcag caatgccgat ggtgaaaaat
     7441 ccgccgtttt ttgcgccttc cagtgcgtgg agcgagtcct ccaccacggc gatctgccgt
     7501 ctgcccaggt gcagtttctg cgccgccagc tgaaagatct tcggctgggt cttgggtgtg
     7561 ccggcatcct gttccgtgag caggaaccgg agccgttccc agacgtgcag ccgtcgcagc
     7621 gccgcctctg ccagttcgcc gtaggtcgct gtggcaacgc cgtaggggat gtcctgctga
     7681 tccagccagt ccaggatctc cgggacgtgg ggtttcagct gcaatttttc ccggtattcc
     7741 tcagcgacca tatccgccac ctgctgtgcg atctgctccg gggtcaccgg aagatcaaac
     7801 tcctctacga aaaactgaga gaattcctcg atggacatac gctgtaccgt ttctgacaga
     7861 tccggcggca cttccttgcc gtaatgcgcc atgagtctgc cgtctacctg ccgccacatc
     7921 ccggtggagt ccagaagtgt gccgtccagg tcaaagatca cgccctgcag ggtgttgtgt
     7981 tccatagtgt tcccgttcct taaagctttg tgaatctcgg ctggttgtcg tgtgctgtgc
     8041 cggtgaagat cgacttcggc attgcccaga cgctgccgtc ctgttccttg cggtaaacga
     8101 ccaactcttc ctgtgtctct gcgtggcgtg cagagggctg gagcagttcg cagacggtgc
     8161 cgtccagaac gtcccggtat tttgcgcctg cctccaggac ggtaggctcc ggaacggcaa
     8221 ctgcctcggt ttccggctcg gtgttgttga cgatgtcatc ctctaccagg atcatagcgg
     8281 agaacggtgc aacacggatc catgcgccgc cgttttccac ctgtaccggc tgaccgctga
     8341 caacgtctac cagagacggc acgtgtgtgc cgaagcggaa ctcgctgggg cagtcctcca
     8401 ggttcagcgc aacataaacg gttctgccgt cctgttcctt ggcgaacagc agctggcggt
     8461 tccggacctc tacggtgtag taagagccgg tgcgcagtgc cggatatgcg tgatagattc
     8521 tgcccagctt taccaggtgg cggtacagat ccagattgcc gctgcggttc agatcgtcca
     8581 gatccaggca agggcgcaga ccgtcgtccg agttattctc gtgtctgccc atgacgccgt
     8641 attcgctgcc gtagtagatg gacgggatac ccggcataca gtacatcagc gtatagcaca
     8701 ggggcagata ccgctggtca gacagggtgc ttgccagacg gtttacatcg tggttgtcca
     8761 cgaagttgta cagcgcaatg ccctgataga tgccgccgtt tccgaactga cggttgatgc
     8821 tgtagttgat ctcgaaatag ttcttgtcgt tgtgtgagga atagatgccc ttatagcact
     8881 catagttggt gacgctgtcc agcatctccg ggtttgccca gcgcttatag tcgccgtgga
     8941 tgatctcacc catgagccag aattccggat tgcgttcctt gcagaaattg tggatgcggc
     9001 ggaagaagtc gaagtccacg cagtccgcag cgtcaaaacg gatgccgtcg atgtggaact
     9061 catccatcca cagttttacg gcgtcgatca gataatcgca tacgtgccag ttgcgcagat
     9121 tcagcttgac caggttgtaa tagccgttcc agccctcata ccagaagttg tcgccgcagg
     9181 gactctgacc gccgaagttc agaccggcaa accagtcgca gtattccgat gcctgtccct
     9241 tttcctggac atccttgaat gcccagaagt tgcgtcccac gtggttgaat acgccgtcca
     9301 ggatgacccg gatgccgttt ttgtgcagtt cgtcgcagat gtgggcaaac atttcgttgg
     9361 tgcccagccg gcggtcaatc acccggtagt cggtggtatc atagccgtgt tccacagact
     9421 cgaacaccgg tccgaagtag actgcatcaa cgcccagttc tttcagatgc ggaatccagt
     9481 ccagcacctt ttccagccgg ttcaccggtt cgccgccttc gttgtgcttc ggtgcaccgc
     9541 agaatcccag gggatagatg tgatacatta ccgcattttc ataccatttc atttttcttt
     9601 cctcctactc ctattatatc acaaacaggg cgaatcccct gttggattgc aattaaggca
     9661 gacctgtttc aaagcaaatt tacttcacag acccttccag ataatacgtt gcctccatat
     9721 ccgccacatt gaccgccagc gccagaggga acatctccaa tgctctgccc acgctgtttt
     9781 tgtcctcgtt tccggaaaag cccatgtgat agcggatggc gaacgcctcg tcccgggtga
     9841 gccgcataaa gctctggatc atgtacacgg atttttcccc gtgtccgtag ggcagggtgt
     9901 cacgcacggt gtaatacggc accttttccc atacgccctg gtcgtttttg gcattccggt
     9961 agtccaccgc atagaaccct accttgcaga tatcgtggag cagggctgtc actgcaatgg
    10021 tctccggtgt gtagtccatg ccgtacagat ccttggtgcg gggacgggag agatagtcct
    10081 tgaggcattc atacacattc agactatgct ccaccagacc gccttcatat gccccgtgaa
    10141 accgggtgct ggacggcgct gtaaaaaagt cgctggcgtc tgagtccagc cactccagca
    10201 gccggtctgc gccacggcgt ttgatgtttt cctgataaac ggcaataaat tcttcccgtt
    10261 ttgtcacggc attccaccgc ctttctcttc actgttgttt attataccac atatccagaa
    10321 aattggcaag cgttccgcaa aaattgtttc aaaatcatga aaaagccgca ggcggagaaa
    10381 aaacctgcgg cttttttcaa aagggatgaa ctgacagacg gaaaaagcat ctgccatggg
    10441 aaatgtcaaa gtctgtttta tatctgacgt acacagattg tttttcaaga gatgaaagta
    10501 aactctgtgc ggtagaaatc actgagaagc agattcttca tcaggcattg gcgggatata
    10561 ctgcgtatag ttctctgcca gatcaaagtc attgatcgtt tgtgtccgat aacctttgtc
    10621 aatgggaaca gtaaaaactg tacctgctgc gtccaagttg ttttttcctt ccggaacaat
    10681 acgaatggtc agttccgtag catcagtcaa tgctgcttcc catacagaat tctgaaagga
    10741 aatgtctgta tcaatattcg cgtctatcat atcataagag ttaccgttct gatccgtgac
    10801 atacatcaca tcgtagtcat caaatacagt atcctcagaa aaacggatct ctgtgctaaa
    10861 cggagaaaca gtcacagatt ccagcgtata tccgtctttt gtttcggtaa ctggatagat
    10921 gtccgttcgg ctggtatccg cctgtatctc cgcagtcaga gtaatatcca caggaataac
    10981 acccagagat ctcggttcac agctatatgc cgtatcaccc ttggcggata cgccggtttc
    11041 aaaccatgac caactagttg catctattcc ctgtaagaac gaaaacgaaa gctgcaacgg
    11101 caggcttgct gtagtgttcc cctgaaatat ctcactgcat tccgccgaaa gcacaatact
    11161 atattccgca tcgccagact tcacaaatcc ggcaatgctg gtattttcct tgtcatagta
    11221 tgcaccctct gcctgttcag aaagcgttgc cagcccaccg gtttttgtac tcatagtata
    11281 tgtaccggta cttccgataa atgacagctt ttgtccattc agtgtgccgg taaactccga
    11341 ttcaattccg gtgctgttct gcaatgcttc tggcaaattc gttaatctta gcgttacact
    11401 caggtggctg ccgtcgcagt agatgttttc catgcgaatc gtaccgtctt tcgttttttg
    11461 catttcgtct gttttttccg cataggaagt cagatcgttt ttctctatgg gactttttgt
    11521 gtaatatttt tcattgctga aaaaggcata gatcccctta acagcatcgg acgaagccac
    11581 tgccggaaca gcgattgcaa tgctcagcac gccggcagct gccacagcga gaaaagtgcc
    11641 gactttcttt tttgcagaac atctggttcg catcattttt cgctccattc tgtgttttct
    11701 tctctggtat gccttggaaa actgcggttc ggccgcgtcc gctgcaaacg cttgttctac
    11761 aatttttttc cattcgtctt cattataagg aaaatagctg ctcataagaa gtcactcctt
    11821 tctgctttat catggctttt ctggcacgtt caaagcgttt ccgtgccgcc gcctccttga
    11881 tgccgcactg ccgggcgatc tcccggaacg agaggttttc ctgacagcgc agcagaacga
    11941 tcttccggtc ggtctcgttt aggacctcca tcatctctgc cagcaccgcc tgccgcatcg
    12001 cctgttcctc ctgctgttcc aaggggcatg gcggcgcatc ctccggaatt tgcagaaatt
    12061 tatcgtccca cggagaacgc cggtcggatt cccgggcgtt tttccggtac tggctgatgg
    12121 cagtgctgcg gatgatctgt atcatgtact gcctggtctg cggactctcc ggatcgccga
    12181 tcttgtgcag attggcgatc acccggcaaa atgcctctga aacggcatct tcggtctgct
    12241 gggtgtggtg caggatggcg taggcaatgt ggtacatggg cttttcgtaa attttataca
    12301 gcgtgtgcag tttttctttt gccggctgca tctgtttcat ctccttcccc atttatcgga
    12361 cgtcctatca gatataacgc acaaggacgg cgattgtgac agaactattt taccggacgg
    12421 cggcagcgga tgtcaagaga aaaccatttg acacaactgg ggaaatatgc tatactagct
    12481 tatatcacac tttacatttc atacacattc atgttgccgg aatatggcag gaggaattca
    12541 tgagaaaacg agatcgggtg catctggggc gcatggggat ggcgctgctg attgcaggat
    12601 gttgtctgcc gtggggtcag atctctgctg cggcgcagcc ggcgatgccg gaagtgctgg
    12661 aagagacctg gaagcaaaac gacggcttgc tggtgccgtc agaggaaatg gcggagacgc
    12721 tgccggattc cacagaggag gatgcggcgc tggataattt ccagggcgca tcggcgctgc
    12781 cgtcgtccta ttctttgctg gatgtggacg gcgtctccta tgtcacttcg gtaaaagacc
    12841 agggcaatac cggactttgc tgggcatatg cggcgctggg tgcctgtgaa tccaatatta
    12901 tgtaccaggg tctggagatc ccggaccgct ggcgtgacag caacggggaa ctgaactttt
    12961 ccgaggcatc gctgggctgg tatcctttta ccaaccatct gctgcccggt gataccgcca
    13021 gcggcgacta tatcgtcatg gcgcaaaagg gtatcagcgg cggaaatccc accattgccg
    13081 gctatgcgct ggcagcaggc atcggcacac agctggaaca gtatgcgccc atgtcccagt
    13141 ggaaacaggg ctattccgag tatcagcggt ataattccta ttaccggatg aagagttccg
    13201 atgttttgtt acaggtaaac agcagttcca cggcgaccat ccagcagtgg atcatggaga
    13261 gcggtgcagt cagtgcggcg ttttattcca agagtacctt ctacgacaac ggcgagtcca
    13321 tcgcctatta tcagaacagc cacagtgcca cagatgctga ccacgcagta ctgctggtgg
    13381 gctgggacaa caattattcc cggaagaatt tccgacccgg aaatcagccc aagtttgacg
    13441 gcgcatggct ggtgcggaac agttggggcg atgatgatgc ggacggcggt tatttctggc
    13501 tctcttatga ggaactgtcg ctctgcgagg cggcgagatt cctgatgacc gatgccagcg
    13561 aacacaccac ctgctatcag tatgacggca gcgtctccta tgccaacgtg aagtttccgg
    13621 cggcggcgaa cgtgtttaca gcagaccggg acggtgtgct gactgatgtc atgtttccgt
    13681 tttcctcttc caatccccag agcggctatt atactgtgtc cgtctatcgg ctccgggaga
    13741 atgccaagtc gcccatagac ggcgaaaagc tgtgtaccca gcgtggcacg gtgcagtacg
    13801 gcggctataa gagtatctcc ctggaaagcc agggcattac gctgaaaaag ggtgaccgtt
    13861 tttccgtgac ggtggagctg aaccgtgcca aaagcggcag tgaccgcctg tggctctcct
    13921 ttgagtcggt gagcaacgat gtgctggaac gccattgctc cgtccagccc ggacagtcct
    13981 acatttatga cggggatggg gagtggatgg atatgacgga tgtccgcacc tgggtgaaca
    14041 gcaagggaaa ccagccgtat tccaacctgg ggaatgccgc catcaaggca attgtgcagt
    14101 ctccggatgc agaggtaaac cggagtcagc tggatgcggc gctggcatat ggtgcgccgg
    14161 atgccggagc gaatgcactg taccggagcg cttatgcggc ggcgtccgct ctggacgaga
    14221 ctgctaccca ggcagaggtg gacaatgcgg cgcagaatct gcttgccggt ctggagcagg
    14281 caggaaagct ccggtatccc cagaacatct acacgaaaga aaccgcagaa ccgtcgttcc
    14341 tgctgggcga tgcagacgga aacggcaggc tggatgcgtc cgatgtgtac cagtccatgc
    14401 tggcacaggc gctgaacgcc gtgggagaat ccagcggtct taccatgtcc caggctgccg
    14461 cagcagattg tgacggaaac ggcgtcatca attccacgga taccttctat ttgatgctgt
    14521 actgcgctta ccgtggcgtg ggaaaaccca tgacctggga agagatctta aagatatagg
    14581 acggcattgt aaaaatacag aaccaaaaac aggacagcgt tcccagaaaa aagggacgct
    14641 gtcctgtttt gatttgtgca ttgctgtagg ttcagaactc gttttcacag gaaagacggt
    14701 cgaatctgtc cggaaagacg agtccctgcg gtcaaatggt tatctgccgc agccgcatcc
    14761 acagtcgtca tcgtcgtcgc cctgctggtt ccggcagttc tgtgcagact ggttctgctt
    14821 ctgctgtgcc tgctggttct ggcgattctg gctctgctgc tgctgacggt tctggctctg
    14881 attctggttc tgcttggaca tttctgtctc cgtttctccg cacacgggct ttctcctgcg
    14941 atttttccgt gtgcggagaa agaaatcaca gggcagcggc agatttttgc cgctgaggat
    15001 agattgcccg gaaaagggga aaatatgcgc agatacggag agactgcctg cggatgcttt
    15061 tcaaaaaata aagtttacca gccgcaccgt ccaggaaaca gcgccgcaca gcaagatgcc
    15121 cgccagtgtg ggaatgcacc acgccgccag cgtccacttc cagctgccgg tctctttgcg
    15181 gatggtgaga caggtggtgg agcagggaaa atggaacagc agaaacagca gcatacagag
    15241 cgccgtcacc ggtgtccagc cgtttgccgt taaaatctcg tggatgcggc tgaggtcgct
    15301 ggtctccgtc agtgtcgttc cggcggtgta acccatgaga atgatgggca gaacgatctc
    15361 atttgccgga aatcccagga gaaatcccag caggatcacg ccgtccagcc cgatgcactg
    15421 tcccggcagt tccaggaatg cggcgcagtg gtcgaacagg ctttccccgg cgatggtgca
    15481 gttggtaaag ccccacaaaa gcgctcctgc cggcgcagcc accagacagg cacgccccag
    15541 cacaaagacg gttcggtcta acagcgaccg gagcaggacc ttcccgatct gaggtctgcg
    15601 gtacggcggc agttccaggg taaaggaaga gggaatgcct ttcagaatgg tgtgggagag
    15661 cagccgggaa acgccaaatg tgcaaaagat gcccagcagg atcagcccca gcagcagcgc
    15721 agccgagctg acggtgcggc agagtccgct gccggcggcg aagaacaggg agatgcaggt
    15781 aatcatggtg ggaaatctgc cgttgcaggg aacgaaattg ttggtgagga tggcgatgag
    15841 ccgttcccgt ggggagtcaa tgatgcggca tccggtcacg ccggcggcgt tgcagccgaa
    15901 tcccatgcac atggtgaggc tctgcttgcc gcaggcacag gcacgctgaa aggaacggtc
    15961 caggtgaaac gccacacgtg gcagataccc gaagtcctcc agcagtgtga acaggggaaa
    16021 gaagatcgcc atgggcggca gcatcacggc aatgacccag gtcagcacgg tgtatatgcc
    16081 gtccaggaga aagctttcca gccagcccgg cgcagacagc agttccagca gccgggaaag
    16141 caccagcttt agctgtgcaa acccggcagc gagccaggtg gacggatagt ttgcgcctac
    16201 gatagtcagc cacagcacca gtcccagcag caacagcatg accggaatgc cccatttccc
    16261 ggcaaggatc cggtcaattt tccggtcggc ggcatcggcg tgttccggct gttggacgac
    16321 ccgggcggcg atctcttcgc tgtgggtgac aacggcggcg atcatggcat cggcgcactg
    16381 ttccggggag atcaggcact gtgcctgccg cagcgcatcg gcaatttcgc cggtctccag
    16441 agaaatgccc atgtgtgcgg cgattttttc gtgcagttcc ggttcgggtt ctaacaggcg
    16501 ctttgccgtc cacagcggcg gcagcccgtt tccgttttcc ggcagggcag cctgcaccag
    16561 ttgcagcggt tcttccagct gttccggaat gggaaccgga cagggtgacg gcgcatcctc
    16621 atgcagcaga gcgtggagcc gttcctgcag ctgtgccagc cccttgccgc tccgggcggc
    16681 gacgccaacc accggaatgc ccaggcactg ttccagccgg ggcaggtcga tggtgatatg
    16741 ccgcttttcc gcctcgtcca ggaggttgac acagagcagg gctttgggac aggtctccag
    16801 cacttgcatg accagcagca gtccacgttc cagacaggtg gcatcacaga ccactaccgc
    16861 cgcatccggt ttggcaaaac agagaaagtc ccgtgccaca gcctcttcgg cactcctggc
    16921 acggagagaa taggtgccgg gcagatcggc aagcaaaatg gtctcgccgc agcagacagt
    16981 tgtgccgacg gcgctggcta cggtttttcc tgcccagttc ccggtgtgct ggtgcagccc
    17041 ggtgagggcg ttgaacacgg tggactttcc cacattgggc attccggcaa gggcaacaac
    17101 aggttgttcc cgctgggaca gctccggcgg cagttcggca gatgatgtgc gcatagcaag
    17161 attcgtcctt tcgatagcat ggttttttgc aattctatgc ggaaaggaaa gaaaaaagac
    17221 cgcctgtgaa aaggcggtct ttttcaagtg tgttcgcatg gtttgcgaag tattcaaagc
    17281 atatcttgca tacttagcat acaaaaagaa ataaccgccc ctgcttgcaa ctgcgggcgg
    17341 ttattttaac cacattgtgg gagcagaccg tctgctggta tgctctcttc acttttatta
    17401 tatgcgattt tttccggttt gtcaagtgct tttttccaaa acaaccgcag ctgtcctact
    17461 ttgtctcctc taccagattg ctgatccaga cgcctttttg aatgaggata tagccgatga
    17521 ctactttcag aatatccgcc gcctggacaa tggcatagat gcccaggatc ggcaggctgg
    17581 taaagtggca caggatcagc gccgtgggca gcggcacgca ccaggtaaag acgctgtcaa
    17641 ataaaaaggt aatcatggtc tttccgccgg agcgcagggt gaagtacagg gcgttcaaaa
    17701 atccctgaac agggaagaaa gctgcggtaa tggtaatgaa ccatctgccg tagttccgga
    17761 cttcctgggt ggtgtcatag gctttgggga acacgccgga aacggagatc aggatcgccg
    17821 ccagcagcag acagacgccg ccggtgaacc acatcagact gaacgaatcc tttttggcac
    17881 gctcgtattc cgatgcgccc agcgtctgcc cgatgagaat gcccaccgca gaacccagcg
    17941 ccacaaatac cacattgagc agattgcaga tggcgttgga aatgttcagt cctgccacca
    18001 cttccagccc acgggtggaa tagcactgcg tcagcgccgc caacccggca gaccagagca
    18061 gttcgttgca gaaaatgggc atacccttcc ggatgacctt tttcgccaga gtaccgggga
    18121 caagcagcgt gcggtaaatg ccctttagga aagtgtgttt gttccggcac agatgcgccc
    18181 agatcactac aatgctcatt tctgccacac gtgccagcac agtggcaatg gctgcgccac
    18241 ggacgcccag tttcggcatt ccgcagttgc catagatcag cagatagttg aggacaacgt
    18301 ctaccacaac agatgccacg ccgcctacca taggcttgac gctgctgccg gtctcccgga
    18361 gactggtggc atagacctgg gtcaccacaa agaacggcag cccgatcagc ataatgtgca
    18421 gatactgctt ggcataggtc atggtgagcg ctgcatcgac cgtggcgctt tctccgtgga
    18481 gatacagccc gatcagcgtc tcgtctccca gcaggaatac cagcagtccg ccgatgagac
    18541 aggcggcgct gagccacagc ttgatgcgga atgtactttg aaagccgtac agattgcgct
    18601 gtccgtaata ttgtgcgccg tagatgcccg gtccggcgag tgcgccgaaa atggcgaggt
    18661 tgaacacgaa gatcagctgt ccggcaatgg aaacggcggt cattgcctcg gttccctggc
    18721 tgcccaccat aatgttgtcc accaggctca ccatattggt gatgccgttc tggatcatca
    18781 tggggacagc cagcatcagc gcccgtttgt agatcggatt gttttttctc atgtgggttg
    18841 ttcctcctgt gatgtcaaaa agatagtcat gcagatgata gagcgtttca gggaagattt
    18901 gctaccatta tagcaaaagc gatcgtgtac ttctaataca atttctgttt gttctgatac
    18961 aatttctatt ttgcggtcat atccgtgttg tcacaccgct gccgtttccc acaatgcgct
    19021 gaggcagcac attcagaaaa ggattgccgc cgcttctgcc ctccagcagg aacagccacg
    19081 gctgtgcgtc cggcgctttc gccacaaact gcagccgctt tggttcgatg ccgttttgcc
    19141 gcatggcatc catgcagtct gccagccgtt ccgggcggtt gcagatgcag agtctgccgc
    19201 cgaaccgcag cagccgtttt gccgagcggc agatatcgta gatgttgcac agcacctcgt
    19261 gccgtgcaat tttttgtgcc gtcagctggc tttcgatgcc tgcctggtat gccttgtaag
    19321 gcggattgca ggtgaccaga tccagctgtc ccaggggtgc gccgtcccag agttctttca
    19381 gatcggtgca gaccgggtgc aggactgaac cgatgcccat gctttcggcg atgccctggc
    19441 acatctgtgc gatcgcctcc ggctgaatgt ctacggcgta gatctcccgg ggcggtgcgg
    19501 tgcgctgcat cagaattggg ataatgccgc agcctgtccc caggtcgcag acacggtcct
    19561 tctgccggta ttcggaaaat tcagtcagca gcactgcatc cgtgccgaac cggtggagct
    19621 ggctggtgca gacgatcagg tcgtctgcaa tggtttcata agagaggacg ggcatatgcg
    19681 gcatgggaat acctcctgtt cgagtgatgt cgttgcgtgt gattccggga tagtgtgact
    19741 gcgggtcgat gtgggcatat attattgcgc atcaatctcc ggcattatcg ttaccgtctc
    19801 tgtctccgtc tccccggact gcatcttatc cgggtcacgg atgactaccg cctgctggta
    19861 caccaggctg taccggtctt cgaatttgtc caggtcgtag tccacttttc cctccagcgg
    19921 atcgcagacc gtgacggtgt tggcgttcag atcatagccg cacagcagca cgcagtgttc
    19981 attgatgaga aaaacggcat cctcgccgtt gggcgtggtc cagtaatagc gctcttccac
    20041 atggctggtg aggttgatgg tcgcccaggt gatgaccggc gtgccgtttg ccacctccca
    20101 gagcagcgtc ttgagggaac atccggtgag attctgcacc acctcgtttc cgccctgggc
    20161 ggcgatgtag ttctgcgccg tctccacgat gaccggggca tagcatcccc aggcgttgct
    20221 gttgtgcggt gaaccgacaa acgcctcgcc gaatgtggtg agatacgggt cggactggat
    20281 cagatacttg tccgccatag tcaccttgtc caccggatag ccggcgtagt tcagtgccgc
    20341 tgttagcgtc gtgacttcac agcctgtggg cagttccgga ttttgcagca ccggctgggc
    20401 atccagctga acctggtcag caggcagaga tgctggttcg gtgggcagtg tcagttctgt
    20461 tttcatgggg attgtcccct gatacaggac gctgggcggt gctgtggtct cgcccggcgt
    20521 tgtggtctcc agcgtaatgc tggtcttttg cgtaatgcgt ttggtctggc tgctgtcgga
    20581 acggaaaaaa atcagataaa ccgctgccgc cgccagtgcc aggatcagca gcagcgccat
    20641 gcgccgccgc cgtcccagcc gtttttttga tactttctcc atctaaaata cttcttccca
    20701 aagcggacgc cgatttcgaa acaggtgcgc cgctgttatg attgtgattc cggtgcggcg
    20761 tggtgtgtgc cgccggaaat ggtgatcttt cgccggaaat cggattctcc ctgtccgatc
    20821 accggccgtg ccagctggta gatgtcggtt gcctgggatt ccagggccgc acagcgtcct
    20881 gccgtggggc tggtgtgtgc cagttctgcc agcgaatggg aaaacggcag cagccgcttt
    20941 tccttggaat ctttcagaat ctcccgtccc gtgtcgttga atgccagaat cctgccgtat
    21001 gccggatagt tctggatgtc agccgtgcgg atatccagca ggatgtgcag caggatgcgc
    21061 cgcagccgtg ccataggata gcgcttggtg cggatggtct ggagcagttc ttccagggat
    21121 gcagagcgtt ttgccttgta cagccggtgt tccagaccct gcccgatctc ggcgatatcg
    21181 tggagttcat ccgggtcagt ggtgcgcagc ttgtacagaa tgagccgttc cagatacgac
    21241 atggaggcaa cgtgtcctgc ctgctgtgcc tcccggagtg cgtgccaggc gtaagacggc
    21301 atcatgcgcc ggcagtcgcc gccgtccgca aaaaactgcc ggatataggt ggcgcttgct
    21361 gtgccgtccg gtccgggctg gctgctgttg tgacctgcgc cccggcgctg gatggtcatg
    21421 ggacgcaggg gactgtccat ttcatggatg gcgttgagat aggcgatggc gagggtgttg
    21481 ttggggtgcc gcagcacagc ggcggtttct gcgccgtaga gctgccccac catctgggaa
    21541 agcacctccg gataggaata gcccgaccgg agaaaatcgt ccatcacgtc agcatagtcc
    21601 gttttgcaga cccggcacgc ctctgccgcc tgggagagca gccggatatc gccgcattcg
    21661 ctgccgaacg agatctcctg cacattgccc agcgccgtca gcagcgccac accgcccatg
    21721 gcgaaatttt ccgccggcgc cagggcatac ggcacgggca gttccagcac caggtctacg
    21781 ccgccttcga tggcgagtct ggcacgggtg aacttgtcca gcagggctgc gtctccacgc
    21841 tggacgaaat tgccgctcat cacggcaacg attccctctg cgccagcccg gcgcacctgt
    21901 tccagatgat acaggtgtcc gttgtgcagg ggattgtatt cgcagataat accactgatt
    21961 ttcatgtatg atcctccaaa aaagctgtag aatttcaata aaatgcctca taaaccgggg
    22021 aattttccga aacctcttgc aatttttcca aaaaagctta taataatata tggggacacc
    22081 gccggtgtcc gaaaaaacga tgtcagcggc agccttctgc tccggggaca tatcggaaaa
    22141 gcacattccc gaggggatgg tttttcagat gtgttccggc agacgccgca gaaacgaaaa
    22201 aggagaaatt tcccatgatt attcttgttg tcaatgcagg aagctcttct ctgaagtatc
    22261 agctcatcaa tatggaagat gagtctgtga tcgcaaaggg caactgcgac cgtatcggta
    22321 tcgacggcca tctgagccac aagaccgctt ctggtgtcaa ggtggaaaag gactgcgact
    22381 tcccgaccca tgccgaggca ttttcctgtc tggttgaggc actgaccacc ggtgaaggcg
    22441 ccgtaatttc cagcatggac gagatctctg cagttggcca ccgtgtggta cagggtgcag
    22501 aagtattcac caagaccaca ctggtcaccg atgagatcat cgaaaagatc gacgaactcc
    22561 gtgagctggc tccggtacat aaccacggtc acgcactggc actgtgggct tgccgcaaga
    22621 tcctgccgaa cgtgccgcag gtcgttgtat tcgacaccgc attccaccag accattccgc
    22681 agaaggcatt catgtacggt ttcccgtatg aggactatga gcagttccat gtccgcaagt
    22741 acggcttcca cggcacatcc caccgttttg ttactgcaaa gctggctgag gtcatgggca
    22801 gagatctgaa ggatctgaag gtcgtttcct gccacctggg caacggttct tccatcactg
    22861 ccgtacagga cggcaagtcg gtagatacca ccatgggctt tacaccgctg gacggcgttc
    22921 tgatgggaac acgctgcggc gccgttgacc cgtccgccgt tacttatgta gccggcaagc
    22981 acggtttcgg cgtttccgag atggacacct acatgaacaa gaagtccggt atgctgggta
    23041 tttccggcga gtcctctgac ttccgtgaca ttaccgcagc agctgaggct ggtgacaagc
    23101 gtgctaccct ggcaaaggat atgctggtat acgatatcaa gaaggatatc ggtgcctatg
    23161 ccgnnnnnnn nnngtctgga cgctgtgatc ttcaccggcg gcatcggcga aaatgcaccg
    23221 aactgccgtg aggaagtctg cgagaacatg gaatatctgg gcatcaagat cgacaaggct
    23281 gccaacgatt tcaagggcga gctgaagaaa atctccacac cggattccaa ggtagaggtc
    23341 tgggtcatcc cgacaaacga agaactgctg atcgctcgtg acaccctggc aatgatccag
    23401 aagtaagcga agaacagcat catagaaaat accgcaaagc gaagtctgca ctcggacttc
    23461 gcttttgttg tacccgaaag gagaaaatca tgtccacact ttccacacag gaaaaatccc
    23521 tgctgcgcca ggcgctggct gcccgggaaa atgcctatgc gccctattcc cgcttccagg
    23581 tcggggcagt gctgactgtc gccgacggca gcacattctg cggctgcaac atcgaaaatg
    23641 cgtcctattc catgaccatc tgcgccgagc gcagctccgt ttgcgcagca gtttccgccg
    23701 gcgtgagaca gttccgcagc atcgccattg taggcggcac tgatgcggag actgccgaaa
    23761 acacgccctg tccgccctgc ggcgcctgct tacaagtgct ggcggagttc tgcccgccgg
    23821 atttcccggt gatcctggca gacggcgtcc atcggctcag tgactttctg ccccgtcagt
    23881 tccggctgta agagcctggg caataccgca cacaggcagt ccttacttgg acttcttttt
    23941 gtctgccggg cgataggaag aaccgtccgg gcgaatgaca acggtgttgt tcagctgccc
    24001 tttgaggttg ccgaccaccg actgccggta ttctgtgcgc agtgcgtttt gttcttcctt
    24061 ttccgccggg gtcagaccct ccggcgtgcg tgccttcttt gccaatgcgt tgatgcgtgc
    24121 gatttttttc tcgtccatat ccgttacctc ctgacaagac tgcatattca ttcaatagta
    24181 taccatagaa cggcaaaaag tgcaagcttt ttgcggcaaa aagcgatttt ttccgggtgg
    24241 gatgcgtatg gtgtgccgtg agatgcgccc cacggaaatt aattttcgaa aataggctga
    24301 ttaaaattgc gcaaaattat gaggcttgac aaggaaggct cccctgaaag gggagctggc
    24361 agtgcgtagc actgactgag gggtttccac
//
LOCUS       DAWENO010000009        23616 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_891_length_23616_cov_12.172064, whole genome shotgun sequence.
ACCESSION   DAWENO010000009 DAWENO010000000
VERSION     DAWENO010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23616
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_23616_cov_12.172064"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2019..2028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2986..2995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5058..5067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22075..22084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5140 a         6791 c         6254 g         5391 t
ORIGIN      
        1 aaccgctgcc gcagcacctc cgtgctgttt cccggaccgc agccaatgtc gatgatcttc
       61 cttggctgtt ccagcggaat ccgtgccgcc agatctctgg acggctgtgt ccgctgtgcg
      121 ccgaatttca gatactgcgc tgcatcccag ctgctcatga tgatgccatc tccttttccc
      181 ggcgttccgc ctccagacgg ctgtacacac agccgcagaa gttctgccga tagaggtcat
      241 attctctgga cagtacgatc gagcgctgat agccgccttt tttcttaaaa tccgtgggca
      301 gccatgcaat gccgtactgc tgcgccaatt ccgttccctt tgtattgatg aaaacggcgt
      361 ccttgtgggg actgatggtc agcgtcgtgc agacatagtc cggacgctcc ggctgctgca
      421 aggcaatgcg gaacgtctct tccagacgat gcccgatgca cttctgacac cgcagcccac
      481 gctccggcag gtcttccaga ccttttgcca gagcataaaa ccgttccggc acatagtccc
      541 cgtccacgat gcgcaccgga tgccccaggg gcagctcctg caccagacgt ttcagctccg
      601 ccttccggtg gagatactcc gactccggcg aaatgttcgg gttatagaaa aacagggtga
      661 tgttgaaata ttgactcaga tattccagca catagctgct gcacggcgca cagcagctgt
      721 gcaggaacag cgccggcaca tgatcctccg gcagctgcgc cagaatccgg tcagtttcca
      781 actgataatt gattctggat tgcattctgt gtgcctgcat cagcggcgcc ggctgcaccg
      841 gatgccgtgg tttctgctgc tgtcgtatcg gtggatgcag cactgttgtc accgcccagt
      901 gctgtggtgg ctgctgcgga tgcgctgttg gacggtgtca cctgcaaata attgcagatg
      961 ccctgtgcca gtgccgccgc aatgttgtcc tcgttgtcca gaatccactg tgccacgttc
     1021 gggccgtcgt gatactccac ttcaatgagg cagctggggc ttttgtttgc gccgatctca
     1081 tactgatagt tgtccgaacc gctctgcgag ccgtcgatca gaccctggtc tgcggtaggc
     1141 gtaagcgctg ctaccggatc atacactgcc tgtgccagat tcttgcttgc ctccgaagat
     1201 gcgttgtagt agcagtctgt tcccacgccg gtgccggcat tggtgtggat cgccacatat
     1261 gccgccaggt tcttgtcgcc cattgccgtc ttggtctgga tgctgtcgtt gtctccagcc
     1321 acgatgacgg tcacgccgga ctgttccaaa atcgctgcgg ttttctgcgc cagaatgcgg
     1381 cagacagcag cctcgttggt atcgcccaca taaaaggcgt tgtcctcctg gttggacggg
     1441 ctgagataaa cggtcttgct gtagtcgctt tcggtcggag ccgtagtggt ctcctgggtg
     1501 ggtgtggtgg tcaccgtgcc gttgttcttc ttgttcttgt tgctggaaca gctggagcag
     1561 cacttcacca gcagaacgat cagcagaatg agaaccagca gtaccgcaag aatgcggtca
     1621 taccggacac gacgatggtt gcttttggat gccataagaa ttacctccta taaatgctat
     1681 ttatcttcca gagatttcag tgccttgcgt tccgccggat tgaccttgat ctttccgtca
     1741 cgcagcagcg tcacatcatc ccgtttcacc ttgaccggta catccgattt ttccggaatg
     1801 acctttaagt cgcctgtcag cggattggtc tcgcagacct tcgccttggt gccgtcctgc
     1861 atggtgacga cagcgcccac cttcggcgta atgcggagca gttcctcata gacgttctgc
     1921 tcatacttga gacagcacat cagtctgccg cagcagccgg agatcttggt ggggttaagg
     1981 gacagaccct gttcctttgc catgcggatg gagaccggnn nnnnnnnnaa cgtacattcc
     2041 acgtccacca gattcatttt cagattccga taggcgatct tctgctggca tacctggaat
     2101 gcccgctctt cctttttccg gttttgttcc agcgtgcgcc ggtcctcccg ggtcgccacc
     2161 cggaggatct gcttcagcgg cgccgtcacc tgctctttgg ggatctgctt gttggcgatc
     2221 gccacttcac cgcattccgt accacgggca gtttctgtga tgaccaggtc tccgcttttc
     2281 accttccagc tccccggaga aaaataatac accttgccgc cgttcttgaa ccgaacgccg
     2341 ataatttcaa tcatacacga ttcctttcta ttttaaggca ataccgtatc aagcgctcag
     2401 ccgtccatac agtctgcgca aaacgccgcc agcgccagag gcagattcac gttcgcctcc
     2461 atggcagcat atgttctgtc cactgcctgg tgcatggctt gtccggcgct gctggagagc
     2521 cgttctgcca gctttcttgc gcccggcgca tcacagccga tgccctctgc ccggggatca
     2581 tacttccaca ccatcgcatc ccggatggtg cggtcaaaca ggctgagaaa cagcgccgtc
     2641 cgttcccgtt ccttgcccaa ctgagaaact gcctgtaaaa atgcgtattc attcttattt
     2701 attatactat tgattgcgga ttttgtcaat ccggcgactt cacgaattgg catattttcc
     2761 aaaaaattta agcactgccc gatgttcccg tggaacaccc ctactgcctc tgccgcatcc
     2821 tccggcgaaa cgccgtgttc ctccagcgcc gtccggcact cctgctccgt gaccggcgac
     2881 accgccagcg acacgatacg ggagcgcacc gtaggcaaaa gcgccgtggg cgacgttgcc
     2941 gtaaaaatga agtagccgta agtcggcggc tcttccaccg ccttcnnnnn nnnnntcccc
     3001 gactacagag cccttggcgt ctccctcgtt gggcggcgat gccagatcca tgcagacctc
     3061 acgcaccgta tccaccgaga atccgccacg ctttccgctg tgttccacaa agcgcacatc
     3121 cggatgcacg ccgtccgcca gcatccggca ggatttgcag acaccgcagg gcttttccgc
     3181 cccggtacag agcagttctg ccaggaaccg ctttgccagc gtcttttttc ccagcccggc
     3241 ttctccgtaa aagagaaagc cgtgtgccgc acggtctctt ttcagcatct ggtgcagcgt
     3301 ctcctgcacc tgtgcatttc cataaaatcg ttccatatgc gcctcctact gcaccacgct
     3361 gatctccgca ataccatatc cacggcacac tgcaatgcag tcccggtcga tgcgttctcc
     3421 gctgaggacg atgggaacag ccggcggaca gggtactttc accgccgcac agatgcgccc
     3481 gatgctctcc tccacgggga cgatctcctg gggtgcgagc gccgcctccc ggatgccgca
     3541 caccgtctcc ggacggggta gcgaaaactt ttcctctgcg gcgacacggg tcggcacaaa
     3601 attctccagc agttcctcca gcgtctcgaa atcgaagtcc ttacttgccg ccgaaaagag
     3661 cagcaccaca tgataccggt cagcatactc cacaaagacc tggtgctgct ccagatgcgc
     3721 cgccagctcc tgcccgtcca aatcgcttgc acaagcatct atggtcaggt gcatggggtc
     3781 tccgtctgta aagcagtacc ggggagaaaa ccggcatttc agctgctgaa tgcggttcag
     3841 gacttcttcc agctgtttcc ggaacagggg cgacgccagt tcccggttgc accagtccag
     3901 cgatgccagc atgagatagg acggactggt cgagccgaac atcgccatcg catcccggac
     3961 acgttccggc tcggtgtcaa tgtcgctgcg cacatggaga tacgctgtcc ccgtcagacc
     4021 cgacagcatc ttatgcgccg agtcacagca ataatccgca cccagctgaa tggggtgaca
     4081 ctgccacggc aaaaacgcca gatgagcgcc gtgggcattg tccaccaaaa gcttggcgtg
     4141 ataccggtga cacagctccg ccagtccggc aatgtccgcc atccgtccca gataatccgg
     4201 cgatgtcaca tagacgcacg ccggtctggt ctgtgcctgc aaagccgcct caaaatctgc
     4261 cagatcgtat gttcccgaca aaatgccgtc gctcttcctg ggatagatcc actgcacggg
     4321 aatatccagc agcacacagg cgttcagaaa cgcccggtgt acatttctcg ccgccagcac
     4381 cctgcgcccc tgctgtttca tcagcagcag catggtctgg atgcagaggg tcgagccgcc
     4441 gcaggagtag aacgtatacg ccgtcccata gagcgacgac gcattttttt cactctccgc
     4501 cagaatgccc gatgcctcaa acaggctgtc tgcgccggtg atctccgtga tgtccagcgc
     4561 cgtcgccttt tgcagcgccg gtgcgccgaa tctgccctta tgccccggca tatgcagccg
     4621 aaccggatct tgttgtgcgt aatttatcaa aaagtcatag actggtgtat tcatgatcgt
     4681 gtgattctcc aattttcgaa gaaaacctca tcccaaaagt ccatacgacc gcagccggct
     4741 gcgcaccgtt tcacaggcac ggcggtgcgt ccgggaaacg gtagagatac agacacccag
     4801 ctgccgtgcg atctccggca tggttttctt ctctttatag tataacacga ggatctgttt
     4861 ttgtctcgcc gagatctcgt tttccataat attttttgcg atcagacaag cgacacgctg
     4921 tttttcccag cgttccaccc ggagatcctc cggaaacaac atctcttccc atgcggttct
     4981 ggcggtcatg cgcatacggc atcccctgcc ttttcctgcg ccgcatagac ggcaatgctc
     5041 cgcatactcc attccagnnn nnnnnnnatc catctttctg tagctccttt ccggcagatt
     5101 ttctctgtca accgggattc taccaggatg ttttgcaaaa gtcaagtgta aattctgaaa
     5161 aacagaacat tttttctctg cttacgaaaa aaagcaggtc agccacaagg taccgcctgc
     5221 cgtttccaat gattccggat tactgaaatg cctctaccgg ctgcggtgca gcctccatgt
     5281 cagagcggtc gatgtcatac agatacgcct catcccagtc caggaaaccg gctctgggga
     5341 aataagtgat gcgcagggtc tggaaatccg ccggcacttc catgcagatc caaccgtcac
     5401 gggtttcgcc gggctggatc gggtcatcga atgtaccgcc ctcttcgccg aactgccggt
     5461 agataaaccg tgggccacgg atgctgatgc cggcatatgc ctcgccgtct gccgacaagc
     5521 cgaacgtccg ggagagcata tctgcgacca gttcctcgtc gccgttgttc tggatctcga
     5581 attcgaagaa ggtcagcccg atctcctggt cattttccgt aaaggtatag tccgaaacat
     5641 acgccttttt cagcgttgcc acgacttcct ggtctgtgac ctgttctccg atcttgccct
     5701 cgtgactctc caccttccgc atttcctgcg tggtctccgc agaggtttct tctgccacgt
     5761 cacgcaccga gttttccttt tccttctttt tgccgcaggc acccagagag agcagcaaag
     5821 cactgctcag cagcaggcag actgcacgct gtaatgttgt tttcatattc caattccttt
     5881 ctatccagag acggctttgt ctgtgcaaag ctcgcctcaa aaagaaaaag ccaccccata
     5941 tccagtgtga agccggagtg ggacagcttt ttttgtctgt ttgttccaac aaggcttagt
     6001 cagcctcgat tgcgtctgcc ggacattccg aagcacagga accgcaggag atgcaagcat
     6061 cttcagcgat ctcgtacttg tcagcaccct cagagatagc gtctacagga caggcatcct
     6121 tacaagtgcc gcagctgaga caggcgtcag taattttata tgccatggaa attcacctcc
     6181 gtgtatgttt ccgcagggac acaccctgct actctttatt atagcagatg ccatgcaaaa
     6241 aagcaagagg ttttccaaac atttttccgg ctattcctgg atatgttcca gcgcttgtcc
     6301 gaagatggag gaaatgtgcg catccgccag agagtaaaaa atggtttttc cctcacggcg
     6361 gctgaccacg agccgggact gtttcaaaac acgaagctgg tgggagatgg cggactgggt
     6421 catttccagt ttctgcgcaa tatcccggac gcattcttcg tgctgaaaga ggacgaaaat
     6481 gatccgtacc cgtgtcagat cgccaaagat cttgaacagc tctgccacgt ctgccagctg
     6541 tgactccggc ggcatctgcg ccgggatcgc cgccggatgc tgagaatgat cgtgttccat
     6601 atgtctcgtt cccctttctg cattgtcccg catttgataa gacaatatat ttttatcata
     6661 cgatattttg gaaaatttgt caagagcaaa atcaattttc aagcaaaagc cgggtgtctc
     6721 cgcagaaaca cccggctcgt catttttgtt ttctgttact tcgcatagtc cacgacacgg
     6781 ctttcccgga tcagatttac cttgatctgt cccggataat ccatttccgt ctcgatctgc
     6841 tttgcgatct gccgtgccac cagcaccatc ttttcatcgt tgatgacttc cggcactacc
     6901 attacacgga tctcacggcc tgcctggagt gcatagcact tctccacgcc ctcataggat
     6961 gcgcagatgc tttccagctt ttgcagacgc ttgatgtagt tttcatagtt ttcacttctc
     7021 gctgccggac gggctgcgga gatggcatcc gctgcctgta cgatacaggc gatcgccgtt
     7081 ttcggctcca catcgttgtg atgcgcctcg atggcgtgga tgacttcgtt gttctcgttg
     7141 tacttccggc agacatctac gccgatctgc acgtgagagc cttcgatctc ggcggtcagc
     7201 gccttaccga tgtcgtggag caagccggca cgccgtgcca ggtttgcgtc tacgcccagc
     7261 tccgatgcca gaacgccgca gagcttggac acttcgatgg agtgcatcag cacattctgc
     7321 cgatagctgg tgcggtaata cagccgtccc agcagcttta ccagttcgtt gttcagaccg
     7381 tgtacattgg tctccagaac ggcacgttcg ccctcctggc ggatgcggtg ttccacttcc
     7441 cggcgcgcct tttccaccat ttcctcgatc ttggtcgggt ggattctgcc gtctgcgatc
     7501 agcttttcca gtgcgatacg tgccacttcc cggcggacgt gatcgaaaca ggaaagcgca
     7561 attgcctccg gcgtatcgtc gatgataatg tctacaccgg tcagtgtttc cagtgtccgg
     7621 atgtttctgc cctctctgcc gatgatgcga cccttcattt cgtcgttggg cagcgccaca
     7681 acagaaaccg ccgtctcgga cacctggtct gcggcacact ttgcgatggc gagggagata
     7741 tagctccgtg ccttttcttc gcactcgtcc ttgatctgct gctcatagta ggagatcttg
     7801 actgcctttt cgtgtaccag ttcgctgtcc agggagttga gaatgtactc tttcgcctga
     7861 tcctgtgtca gaccggagat acgctccagt tccttcagct ggctttcctt gacttcttct
     7921 gcctctgcca gcttgcggtc tgcattcttc agccgctgct tgagggactc ttctttccga
     7981 tccaggttgt cggtcttttt gtccagattc tcttccttct gctggatgcg gcgttcctgc
     8041 cgtccgatct cagagcgtct gtccttgatt tctttttccg cctcagagcg gagtttatag
     8101 atctcatctt ttgcttccac catggcattc ttgcgctttg cctctgcctc tttttttgca
     8161 tcttccagaa tgcgtccggc ttctttttcg gcggaaccga tggctgcttc tgcctgctgt
     8221 ttccgctgtt cggcgccgtt ggctacgccc ttgcggtacg ccagaaatcc ggcgaccgcc
     8281 gcaccgatca gcagagcgac cagacacagc acgatcatca gcgcccaatt catctcttgc
     8341 atctctctgc actacctcct tatgctgatt tgcagggagc gccctcctga gaaaacgatc
     8401 cctcagtgtc tatctgtata caacacagca gcgttttcgc ccaaaggggt atgtgcgtct
     8461 cgtattcact tgttctcttc acaggcatca cggaaaaagc cgcagcattc tccgattctg
     8521 cggtttttgc ggcgttctct gccggaagat tcatttcatg tatgccgcac agccggttct
     8581 cctgcccggt tttgtgccac tacaagccca tttcctagaa aaaggcaatt ctcattattc
     8641 aagtgtaccc cacaaaaaac gtgcatttct ttttataggg cgcacgctct aagagccttc
     8701 atactctatc ggaactcgtc cggcaggcac gccgcccggc cggaacggga ttcatgaata
     8761 aaacaaaacg cacacgataa aacgcagcgt caacccggca tcaccgccgg gaatcgtttg
     8821 taatttgata caacattttt attttacact gtttccgtga aattgtcaag acgtttgcag
     8881 aaagattcac attttttctt gcaaggcata caaaaagccg acgatgggat gccaccgccg
     8941 gcttgccgta tcggataatt ccgtctttta cttcttctcc gaaatgcgca tggaaatatc
     9001 aaagcacttc acagaatggg tcagtgcgcc cagggagatg atgtccacgc cggtctccgc
     9061 aatgccacgg atggtctccg ccgtcacgtt gccggatgcc tccagcatca ccttgccgtt
     9121 cacacgctgt accgccttgg tcatatcctc acagctcata ttgtccagca taatgatatc
     9181 tgcgccggca gaaagtgcct cttccagttc ttccagcgtc cgcacctcga cctcgatctt
     9241 caccatgtga ccgatcttct tccgcagggc agccactgcc ggcgtaatgc cgccgtatgc
     9301 gtcaatgtgg ttgtccttca gcatagcgcc gtccgacaga ttatagcggt ggttcttgcc
     9361 gccgcccacg gtcacggcat acttttgcag cggacgcagt cccggcaggg tctttcgggt
     9421 gtcagtgatc tgcgcattcg ttccggcaac cagctccacg cactggtgcg tcgccgtggc
     9481 aatgccgctc atgtgctgca aaatgttcag cgccgtccgt tcgcccttga gcagtgtctt
     9541 ggtgctgccg gtcatggttg cgatgatatc gcccttcttg acctgctcgc cgtcatggat
     9601 acggatgtcc atggagaaat tgccgcccac cagttcaaac acacgcttgg cgatgtcgat
     9661 gccgcacagc acgccgctgt cctttgccag ataatatgcc tcgctggtgt ggctgtcgtc
     9721 cagcagattg tcggtggtca catcaatata gtggatgtcc tcatccagtg ccgtagtaat
     9781 cagtcggtca atataggttt gctgtaacat gggtattttt tcctttcgtc ttgcttaaaa
     9841 cacatctaag gtacttctta aagtacttct ttcagaataa tatatgcaac tgttgccaga
     9901 gactttgcct ctacatagtc tgcatccagg atatagttgc cgttcagcag ctgctcccga
     9961 atttccgtga tgcgccggaa tccctccggc agcttttcct tgttcgggat aacaaaaaag
    10021 ttgtcctgca tgatctgacg aatctcggtg cgcagtccct tgggaatctt cacagcaccc
    10081 ggctttgccg gaaattctgc cgcctggtat tccttcgggg catccggcag cttcttcgca
    10141 atatccagcg ctgcctgtct ggagaacacc agcgcctcca gcagggagtt gctggcgagg
    10201 cggttgttgc cgtgtacgcc ggtatgagag cattcgccgg cagcgtacag attggggacc
    10261 gtggtctccg agttgtcgtc tacctggatg ccgcccatca gatagtgctg gcacgggaag
    10321 atcggaatcg gctccttggt catatcatag ccctgttcca taaccttttc gtagatcatg
    10381 gggaaacggt tcttgacaaa ttccgggtct ttataggaga tgtccagata gaagttgtcc
    10441 gaatgggtct cccggctctc cagaatgatg gaacgggaaa ccacatcacg gggcgcaagc
    10501 tccaggcgct cgtcatagcg gtccataaag cgttccttgt tgcagttcag cagatatgcg
    10561 ccctcacccc ggacagcctc cgagatcagg aaacactctc tggtgtggtg gtcgttgaaa
    10621 gcagtgggat ggaactggat cagatgcagg ttccggatct ctgcgccgtt ctcataggca
    10681 aacgcaatgc cgtcgccggt tgcgatcttg gagttggtgg tgaactggta gacccgtccg
    10741 atgccgccgg tcgccagcat cataaagtgg ctgctgtaaa cgatgcagga accgccctgc
    10801 atcacattca ccgagaaacc ggtctccgtt ttctgcattc ccatcatcat agcgtgttcc
    10861 agaatggtca cattgggcag cgtctttacc tttttaatca gcttgtccag gatctctgcg
    10921 ccggtggcat ccttatgatg gaagatccgg tgacggctgt gtccgccctc cagcgtccgg
    10981 tgcagttcgc cgtccggtgt ccggtcgaag tccacgccga aatccaggat tgcctggatc
    11041 tcatgggcgg catgggtcgt cagaatatgc accgtctccg ggttgttggt aaaaccgccg
    11101 gcaaccatgg tgtcgtgttc gtgcagttcg atggaatcct ccggcgactt gtacaccccg
    11161 gcaataccgc cctgcgccag cgcagagttg cagagggtca gttcctgctt gcacagcagc
    11221 aggacacggc agtccgacgg cagattgatc gcaccgtaca gtccggcagc accgcagccg
    11281 gcaataatca cgtcaaaatt gttttccata acgttcacgc ttcctatcct ttattgcggc
    11341 atctttctgc cgcagaatct tttttctgcg ccggcgacag caggatattc gcacgccggg
    11401 cagcagtatc tcctccacag cggctcacgc cgccacgttt cccttttagt atagcatacc
    11461 cgttcccatt tgtcactact tttgcgcaca tttcggaaat atgcgcaaaa tccgggtgga
    11521 aagctgttaa ttttttgaca agcaaggcga aaatccgtgg aagagcggcg gaatctctgt
    11581 gaaaatgtcc ggcgcagtgt gctttcggcg ctgtactccg ccttttgaaa aatcagatag
    11641 aaatttatgt tctttttcca tctttttcgt tgacaattgc agtgaactgt gctatactag
    11701 atgtatgata tcggactgac gcactgcaca agattgtgcg gtatggtcag aaatacattt
    11761 tgaggagttt acttatgatt tgtcctaact gcggcagcaa caatcccgac ggcacagcgt
    11821 tctgtgcgaa ctgcggttct cccgtttcca acggcgcccc gaacaacggt gcgattccgc
    11881 ctcaggcaca gccgaatttc cagggcagtg cgcccaacgg cggcatgaca ccgcctccgg
    11941 tatttaacgg caacccgttt ccgggcggac acatcgagca gagaaacatt gcgctctgca
    12001 tcatcctgtc catcatcacc tgcggcatct acggtctgta ctggatctat aagctgacag
    12061 aagacgtgaa cacactgaaa ggcgacccga acgcaacctc cggcggcgtc gtgatcctgc
    12121 tgggcatcat cacctgcggc atttatatgt ggtactggct ctataagcag ggcgacaacc
    12181 tcgaccagat caaggcatcc cgtggactgc ctgccggaaa cagcggcatt ctgtatctga
    12241 tcctggcgat cgtcggactg agcatcgttt cctatgcact gatgcagaac gagctgaacc
    12301 agctggcata atccaaaaca aaagttgaaa tacaaaagct gcaccggagt ttccgtgcag
    12361 ctttttctgt tcggaggtgt ctctttgctc cagaatctgc gtgccttttt ccgcacttac
    12421 cgcaagcagc tccagacggc aggcgttttt ctgctgctgg ggctggcata ttttctcctc
    12481 acccagctga cgccgctgcg catcccctgc attttccaga aaatcacagg gtttgcctgt
    12541 ccgggctgcg gcatgaccca tctctgtatg cagctgctgc ggctggactt tgccggtgca
    12601 gccggcgaaa accttgccgc cgccttgctc gctcctctct ggctcgccgg cattctgata
    12661 cgggcgctgt ggaatccagc ttggttttcc aaaggtagcc gggcggaaaa catcctgctc
    12721 tggggcagcg tggcggtact gctgctcttc ggcgttctcc ggaatctgcc cggactggaa
    12781 tttctgctcc cgtcttatct gcgctgacgc acccattttc ccctcccgga atatgatgat
    12841 aaaaaatgca aaacaaaagc ccatgtttat tttatccgtt cacgggcata atagaaacgg
    12901 cagacgtttt cagaacgtgt ctgcgccgca gaatcaacaa cactcgcagc accaactctg
    12961 ctgctgttgc ttttatgaca aaaggaggga tttccatgct ggtaaggcgt ggtgaaattt
    13021 attatgcaga tctcagtcct gtcgtaggtt ccgaacaggg cggtatccga ccggtactta
    13081 ttgtgcagaa cgatgtcgga aaccggcaca gccccaccgt gatcgctgcg gcaatcacca
    13141 gccgccggga aaaggcaaag ctgcccaccc acattgacgt acaggcagcg acctgcggac
    13201 tcaccaagga cagcatcgtc ctgctggagc agatccgcac cctggataag cggcggctga
    13261 aagaccgcat gggcgaactg gatgagccgt ccatgacacg ggtagatcat gcactacagg
    13321 tcagtttcgg acttccgctt taaggcaata ccgcacaaag cttgtgcgta agatgcgcag
    13381 ctggattttt cggtagattg catagaaatt ccgcaggcat actgtcggtc gcaatcaatc
    13441 ctagtgcgac actcctttgt tctcgggcag aatcctgcct cgaacgcagt cgtatggcaa
    13501 ggaatttctg ggcaatatga cggaaaatca gcaagcagat tgcgtgcaaa gccgccaggc
    13561 gggctttgtg cggtattgcc ttaatgatag gtaacgtaaa aagagcgtac acccgttcgg
    13621 gcatacgctc tttctttttt gtgaaaatga taagcaaccg cttactttgc agcgttcagc
    13681 ttctttgcca gctgagactt cttgcgagct gctgcattct tgtgcatcag accctttgcg
    13741 caagcctggt caacacgctt gattgcaaca cgtacagctg cttccttgtc agctgcgttt
    13801 gctgcgcagg ctgcgtcagc cttcttgatc atggtcttca gattggactt gcgagccttg
    13861 ttggcagcag tcttggttgc acttactttt acacgcttct tagcagaacg aatattcggc
    13921 atagttcaga tctccttttt caacaatcaa aataatgggt agaattccct cgaatcaccg
    13981 gattctgtac gtcatacact aatgacagca ttgacacggt actgccattc agtggggaaa
    14041 tttccccgca atttggaacg accaaatgct ggcgctcatc gatgagaacg ccgtgactaa
    14101 attctattat aacacagccc gtcacaaatt gcaataggta ttttcgagga acgccacaat
    14161 tttttcccgt gaaaagccag cagtttgcac aaaatccagg aggtttcgcc atgacacagc
    14221 agtttcgcac cgaccttgcg gtagaacgca ccgccgccgc acccgacctg ccgggaatca
    14281 tccaggagac ccgtggaaaa gcattttccg tcacagagat ccgcatcaaa aacgacgtct
    14341 gcggcgcacc catcggcaag cctgccggac gctatgttac attagaggca gccgccatat
    14401 cccggagcag cgccgactat gaggcaatgg cagaggaact ggcggaggag ctggggcatt
    14461 ttctgccgga agagggctgt gtctttctcg ccgggctggg gaaccatgcc attacgccgg
    14521 acgcactggg tccacgggtg gcgtcgaagg ttctggcgac acgccatctc cggacggcgc
    14581 tgtccgaaga ggaactttcc tatctgccgg agctgcgccc ggtttgtgtc ctcgccggcg
    14641 gcgtgctggg gcagaccggc atggagtcgg cggaactgat tgaggcggtc tgcaaacaga
    14701 tccagccggc ggcagtggtg gctgtggatg ccctcgcctg tgcagagctt tcccggctgg
    14761 gaaaaacgat tcagatgagc gacagcggca tctctcccgg cagcggcgtg gcgaaccgcc
    14821 gggcagagct ttcccggcgc accctgggcg taccggtgat tgccctggga atccctacgg
    14881 tggtagacct gcacactgcc gtccagggca tctacgggaa aagcgtcccg aaagatcgcc
    14941 ccaatatgat ggtcacgcca agggacatcg accggctcat cgaccacgcc ggagatctgc
    15001 tggcgtgttc tctcaacatg gcgctgcatc ccggcatgac ctttgcacag gcggagggtc
    15061 tgggataggc gggatactat tatataaata taacgaccaa aacaaaattt ttgcttgaca
    15121 aatccgagca aatgacgtat cataagaaat agaaaagggc acgccggcag acggtctgct
    15181 cccacatttg gtgaaaagga taatcgccgt gattggagtt tgagacgatt atttcttttt
    15241 atatgcgagg tatgcaaagc gcgtaaaatc aattgtggat catcgcaccg atatttccgg
    15301 cattgacaga agtgtacaaa aagccgggga ttttcggtgc gatttttttt tgcaaaaggg
    15361 cttgactttt tctgtcgggt ttggtataat aggtgtgtta tccttgctca tgcgctgcat
    15421 gggcgaaatc caaaaggagg tgtattgagt atggcaaaga aacaggaatc ctacgaactg
    15481 atgacggttt tcagtctgaa gaacggtgag gaagctgcta aggagctggt tgaaaagttc
    15541 cgtgagaaga tcgcatcttc tgccacactg gacgaagtaa acgagtgggg caagcgtaag
    15601 ctggcatatc cgatcaacga tgagccggac gggtactaca cactgtatac ctttacgtcc
    15661 actccggatg ttccggcaga agtagagcgt gttctgaaca tcacagacgg cgttctgcgt
    15721 tctctggtta ccgtaaagta agaccaattt cagaaaggga aacgatatgc tgaataaagt
    15781 gattttgatg ggacggcttt gtgcggatcc ggatttccgg cagacacaga gcggcgtcgc
    15841 tgtctgccgc atccgtgtgg cagtggatcg gcgcttttcc aacaaacaga ccggcgaacg
    15901 ggaagctgat ttcattagtg ttacctgctg gagacagcaa gcggagtttg tcagccgtta
    15961 tttccacaag ggcagtatga tcattgtgga aggcagtctg cgcaacagcg attatacaga
    16021 ccagaacggt gtcaagcact acagcatgga cgttcaggct gacaatgttt ccttcggaga
    16081 gtccaagcgt gccgcacagg aaaacggcgg cggataccag ggcgcttatc agcagcctgc
    16141 ttatgcacaa gcagcgcctg cacagtctgc cccggcatac cagcagcctg caccccaggc
    16201 agcaccggct ccccaggcac cggcatccaa caatgctccc ggctctttac agctgggcga
    16261 cctgggcgac ttcgaagaga tcctcagcga cggcgaagtt cccttctgat caagcgaatg
    16321 aaaataccgc attgcagata ggaggataaa tcatcatgga gagagaaaga tctcagcgtg
    16381 gcggcaagcg cccccgcaaa aaggtttgta tgttctgtat agatcgtgta gagcgcatcg
    16441 actataagga cgttgccaag ctcaagaagt gcatgactga acgtgctaag attctgcccc
    16501 gcagagttac tggcacttgt gcattccatc agcgtgaact gacaactgca atcaagcgtg
    16561 caagacacat tgcactgctg ccgtatgtca gcgactaagc tgatctgaca gctcagataa
    16621 cagaagcgtt ccgatttttg aatcggagcg cttttttgtt ttttatgatg tgacgccggc
    16681 agatgcagct cgatattttt gtacaaagga actgacatcc ccgactccac gactcctccg
    16741 tccttcggac acctcctcta agagaggagg caaaattgat accgctctta aagatactga
    16801 cagccgcaga ctggctgaga agtcacgggc gaacgatgtt cgcccctact ataaaacttt
    16861 atagattagg ctcccttgaa aaggcacccg aagggcgtgc cctgtgctga gctggcagcc
    16921 gcaggctgac tgagggattt tgctgtgaaa tctgcaattc aaaaaaattc accagaatcc
    16981 ctccaccgtg cgccggcgtt ctctttttcc agctagggac ttgcactttg ccctattgtg
    17041 tgttataatg agatgatatc agatcatata aaaatgcagg agtgtgatag aatgaaagtg
    17101 gttttgctgg cgcatacccc aacaccggaa cagactgtcg ctgcggcagc ccggctttgt
    17161 tattcagata cagacgtaga ggcactgcgg gagtccatct cccaggaaaa agcggaacaa
    17221 ttcgtggaaa tgctggctgg attcggacac gaaagcccgg tggaacacgt gacgtttacc
    17281 tttggcatcg agggcgtttc ccggtcgttc ctggcacagg tgacacgtca ccgcattgca
    17341 tcgttttccg tgcagagcca gcgctatgtg cggcaggatc acttcgtttt cgtgacgccg
    17401 ccggcgatcg ccgctgatcc ggagctgctg aaagcctatg agagcgccat gcagcagtcc
    17461 atcgacagct acaaccagct ggcagatgcc ctggcggcga agtacgtgcc ggaatttctg
    17521 gcgcagggat gttccgaaaa ggcagcccgc accaaggcgg agaaaaaagc catcgaggac
    17581 gcacgctatg tgctgcccaa cgcctgtgag acaaagatga tcctgaccat gaacgtccgc
    17641 agcctccgga actttttccg gctgcggtgc tgcaaccggg cgcagtggga gatccgggca
    17701 gttgcgacgg aaatgctccg gctctgctgt gaagtttctc cggcgctgtt ccgcaccgcc
    17761 ggtccgtcct gctattgcgg cggatgtacc gagggcaaga tgagctgcgg caaggcaaag
    17821 gaagtacggg aaaagtttga ggtgatccat ggtgaaacaa ggtaagatca ttgtactgga
    17881 cgggctggac ggctgcggca agtccacgca gttcgaggcg ctgggcaagc tgctgacaga
    17941 acagggcaaa accgtaaaat ccatcagctt tcccatgtac gacaagccct cggctgccct
    18001 ggtgaaaatg tatctccacg gcgacttttc cgacacgccc ggcggcgtca acgcctatgc
    18061 cgcatccagc ttttatgccg tagaccgcta cgccaactac aagctggact gggagaaaaa
    18121 ctatgctgcc ggagagatca ttctcgccag ccgctatacc acctccaacg ccatccatca
    18181 gatgagcaag ctgccgccgg agcagtggga cgactatctc cgctggctgg aggactatga
    18241 gtacaacaag ctggaactgc cgagaccgga tctggtgctg tttttgtccc tgccgctggc
    18301 gctttcacaa aaactgttgt ccaagcgcta tgacggtgac gccgccaaaa aggacatcca
    18361 cgaggcagac ctcgcctatc tggaacagtg cagccgtgcc gccgcatatg ccggggcaaa
    18421 gctgggctgg cagtttgtgg aagtttccga cggcgaacac ctctacccca tcgagcagat
    18481 tcgggacaac ctatatcagc gtatacagga ggctttggaa tgctgaattt ccggaagatc
    18541 acactggcag accgtccctg gatccaggag gcgctgaaac agtctgattt tatgggctgt
    18601 gaatacagct ttgccaacaa cctggcatgg tgccgggcgg cagactccca gatctgccaa
    18661 tatgaaaact tctatctgac ctgcagtttc gacaccgagg acggcgcacc ccacttttcc
    18721 tatccctgcg gcagcggcga cctgcggaag gcgattcttg caatggctga ctttgcggca
    18781 gaacggcaaa agccgctggt catcttcggg ctgaccagcc agacgctgcc ggaaatacag
    18841 gcgctgttcc ccggcgtgtt caccctggaa aacaatcggg atgatgcgga ctatctctac
    18901 cgcaccgccg attttgtggc gctcgccggg aaaaagtatc acaaaaagcg aaaccacatc
    18961 gcccagtttt ccaaatacgg ggctgttttt tcggagatca cgcccaacga ctttgacgag
    19021 tgcatcacct ttgcggcaga aagctacaac gccaaagacg gctattcgga cgcatccagc
    19081 gtggcggagc agtatgccat ccacaccttt ttccagaact tcgaggcgct ggaactgaaa
    19141 ggcggtctgc tccgggtgga cggcgagctg gcggcatttt ccatcggcga accaatttgc
    19201 agcaacacct tcggcgtgca tatcgaaaag gcggacaccc gttttcacgg ggcatatccg
    19261 gcaatggcgc aggcgtttgc ggcgcatttt gccatggact acacctatct gaaccgggaa
    19321 gaggatctgg gcattccggg actgcgcaag tccaagctgt cctactatcc ggaactgctc
    19381 ctggaaaaat ggacggcgac ctgcgatacc ccggaaaagc tgctgtaaac ctaggaaagc
    19441 gacacacaag ctttgtgtgg tgctgccccg ggacaaactt gttttatcag atacagaaag
    19501 gataattact atgatctatg tatttcttgc caatggtttt gaagaagtcg aggcgcttgc
    19561 gccggtggat atgctccgcc gtgccaagct ggacgtgacc accgtaggtg tcaccggaaa
    19621 gatcgtcacc ggttcgcacc gcatcccggt gaccgcagac atcaccgccg aagaattaca
    19681 gatcggcgca gatatggaaa tgatcgtgct gccgggcggt atgccgggta cattgaacga
    19741 agaggcatct gagaccgtac aggctgccat cgactactgc gcaaaaaatg accgctggat
    19801 cggtgcgatc tgcgctgccc cgtctatcct gggacacaag ggtctgctcc agggcaagac
    19861 agcgacctgc tacaccggct ttgagaaaga tctgctgggc gcagagatct gcagcgacgg
    19921 cgtgatccag gacggcaagt atatcactgc ccgtggcgct ggcgttgccg tagacttcgg
    19981 tctggaactg gttggcgcta tgctgggcaa ggaggcgcag gcgcagatcc gtgcgtcgat
    20041 cctctgtgat tgaccaggct gcggcgaaac aggcgctccg gcaggaaatg cgcagccgcc
    20101 gcaaagcaat gccccaggca gaaaaagcca gccgggaaca gcgcctttgc cgccgctttc
    20161 tggaaagcag tctctatgcg gactgccgcc agctgttctg tttcgttgcc ctgccccagg
    20221 aaccggaaac acgggaactc ttgcgccggg cgctggcgga cggaaaaacg gttgccgtac
    20281 ccagatgtct cccggaacgg cgcatggcgt ttcaccggct ctccccggaa cagcccctgg
    20341 aaagtcagct ggtctccggc agtttcggcg tggcagagcc gctggaaact ctgccggtga
    20401 tcttgccgga tgccggacaa gcgccgctct gcctggtgcc gggacttgct ttcgatcaaa
    20461 acggcggcag actgggctac ggtgccggct attatgaccg ctttctgcgg gcatatccct
    20521 ttttgcgaaa aatcggctat gcgctgtcct cttttgtgac ggactgcgtg ccaacagaac
    20581 cgacagacca gcgtctggac ggtatcgcta cggaaatatc tctggaggta tggaatggat
    20641 aacaaagact ttaaccgtga caagctgctg aacgacattc tgcgggaaac ggctgcacag
    20701 aaagacgctg ctgcaccacg gcagtctgcc ccgacaccgc cgccgagaac atccgagccg
    20761 gcacaacgtc ccgcatcgga accggctgcg ccccggcaga gccagccgac tgcatcccag
    20821 ccggcagcac aggcatttca gccggcaaac gctcagccgg agccggacag caacacccag
    20881 accaccctgt ttgacagtgc gcagtgggaa atgctctccg gcggcgaaac cgcatcccgg
    20941 agcgccatcg aggacgatcc cctgtacgcc gacctggagg gcgaaagcga accctttcac
    21001 ccggagccgg tacataataa taacaatgcc agtacatcgg caaagcgtcc ccggaaaaag
    21061 cggaaacagc accggatcat cagcgccctc attatgatta tcgtcatcat cggcgtgtcg
    21121 atcctgctgt ccagtgcgct gatcgtctac ggcagagacc tgctgggcat caacagtgac
    21181 agttccacca agatcgtcac catcccccag ggcgcaagca tgaaggagat cgccgagacg
    21241 ctccagcagg cggacatcat ttccaagccg gatttctttg tctttattgc cggcatgagc
    21301 gacaaggaca aggacatcaa gcccggcgac cacgagctgc gtccggatat ggcgtatgag
    21361 accattctga gcgaactggt cagcaagcct ctggacagtt ccctgtccgt ctccgtcaca
    21421 ttcccggagg gcattcgtct ggtggatgcg gcagacctgc tggaagagaa caacgtctgc
    21481 gacgcagacg agttcctgga ccacttcaac catgacagca aattcggcta tgcctatgaa
    21541 gaccacctgc ccagcttcca ggacgaaaaa ttctatcaga tggagggcta tctcttcccg
    21601 gacacctata ccttctacca ggaaatggac gtggaactgg tctgccagaa gattctggag
    21661 aacttcaacg acaagatcac caaggatatg tacgaccgca tggatgcact gaacgtcacc
    21721 ctggatcaga ccatcaccct ggcatccatg atacaggcag aggcaggcaa caccgaacag
    21781 atggcgacta tctcctcggt attctggaac cgtctgaaca acgccaccga atatcccctg
    21841 ctccagtccg accccaccac caagtatgtg gaagaggtca tcaagcccca cagcgaatcc
    21901 tacaacgagg atctgtacac ctcctatgac acctatcagt gtaccggtct cccggcaggt
    21961 ccgatctgca atccgggcat tgaggcgatc caggcggcgc tgtatccgga taacaccaac
    22021 tattacttct tctactccaa catcgacacc aaggagacct atttcgccga gaccnnnnnn
    22081 nnnnatccag gaacacgagg caaatcagaa gaaggtacag ggtcagcagg cagcggcaga
    22141 ggaatctgct gccgccgagg aaaacagcga ggaggacagc gatggcacag aggaataaac
    22201 cggaactgct ggcgcctgcc ggcgatctgg aacggctccg ggcggcgctg gactacggcg
    22261 ctgatgccgt ctatctggga cgggaaagct ttagtatgcg tgccgccgcc agcaatttcc
    22321 acagcgccga gcagctgcac gaggcagttg ccctggcgca ccagtacggc aaaaaggtat
    22381 atctcacctg caacaccctg ccacggaacg aggagattcc catgttcccc cagtttttgc
    22441 aggaggcaca ggatgccggc gcagatgcgc tcatcctggc agacctggga ctgctggcaa
    22501 tggcacgaaa gcttgcgccg gatatggaga tccatatgtc cacacagacg ggcatcgtca
    22561 actacagcac ctgccgggcg ctgtatgaca tgggcgtgaa acgtgccgtg ctggcacggg
    22621 agctgtccct ggaagagatc gccggcatcc gggcgaagat cccggcagaa atggagctgg
    22681 aggtctttgt ccacggagca atgtgtatgt ccttttccgg acggtgtctc ctgtccgcct
    22741 atctcaccgg acgggacgcc aaccggggtg cgtgcgcaca gccctgccgc tggaactatc
    22801 agctggtgga atccaaacgg ccgggacagt atttccccat cgaggagacc gacgaaggca
    22861 cgtacatttt caacgccaag gacctgtgca tggtggagca tctccaggaa ctgctggacg
    22921 ccggcgtgga cagcttgaaa atcgagggac ggaacaaatc cgcctattac gttgccgtcg
    22981 tgaccaacgc ctatcgtgcg gcgctggatg ccgccatggc tggaaagccg gcgccggaat
    23041 gggcgctgga tgaggtggtg caggtcagcc accgggaata ctgcaccgga tttttctacg
    23101 gcagagaaaa cgcctgtcag cgctatgcag acagcagtta tgtgcgcaat tgtgatgtcg
    23161 tcgccgtggt gaaccgctgg gaaaacggca ggctctatct cacccagcgc aaccggttct
    23221 tcgccggcga caccctggag atcgtctcgc cgggacagcc gccggtgacg gtgacggcgc
    23281 agggactgca aaacgaggac ggcgaatcca tcgagaccgc aaaccatgcg atgatgcagt
    23341 gttccatctg ctgtgaccag gcttttccgg cacgcagtgt ggtgcggaaa ccggtggaat
    23401 aaggaggctt tttcatgcgg cgacccaaag caattctttt ggcggcggtg ctgaccggcg
    23461 ttttgtccag cggctgcacc cgaattcaca gtgcagatga actgacgcag gacaccagac
    23521 cggagactac gccggttttt acagacacgc cggaactgaa cttttccgcc gacggcgttg
    23581 acccgtatgc caacgtgata cagtactatg cggagc
//
LOCUS       DAWENO010000010        23546 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_893_length_23546_cov_10.653569, whole genome shotgun sequence.
ACCESSION   DAWENO010000010 DAWENO010000000
VERSION     DAWENO010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23546
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_893_length_23546_cov_10.653569"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4094..4103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6132 a         5871 c         5311 g         6222 t
ORIGIN      
        1 cactgctgta cattagcgcc gtttgccgtg gatgcgtcag ccacttccac atagcaggca
       61 tcaccggagg cgtgggtcag aatgcagtag ctgccgtcca ggttcttggt aaagcggaac
      121 agctgggagc tgtccttatt agaccacgcc gcaatcgaca ggtttccgcc gtttttgctg
      181 ccctctgcct tcagcgcata gccgctgtcc tgccgggagc gaatccagta atagttttcg
      241 gagccgaaag cacggagtgt ccatttctgg cagtccagcc cgttggatgc ccactgctgc
      301 acatttgtgt tgtctgccat ttttccgccg gcaatatcca tcaccagacc ggaaccggca
      361 ttttcaaaag tatagatcac atctgtgtcc atcgcacagc cgggatctgc tgccggctct
      421 aaaatccagt tctgacagtc tgcaccgttg gtttcccact gctgcacatt ggcgccggag
      481 gtcttggagg catcttccac ctctaccacg gaatttccgc cggaaacagc agttcggatc
      541 tggtagctgc cgtctccgtt tttcgtcagc ataaactgct gattgctgcc gccgttatac
      601 gtgtatatgt cgatattcgt cccgtttgcc gtctttttcc cggcaacatc cagcacatat
      661 gtgccgccgt ctcctactgc cgaaaccagg tagtaatacc cgtctccggc actgcacagt
      721 ttccagatgt catgaaccga tgttccgtct gaaccccact gctgcacgtt agcgctgttc
      781 tttgccgctg cacctgccac ctgcaagtac aagccggagt tgacattccg gatgcgataa
      841 gtggaaccct ctgcgaatgt ttccgccagg ggtgcgctgg gagattcggt gtagcctgcg
      901 ctcggcacac cggaaaccgc ataccggaga tacatcatat ggttccggtc attctggcag
      961 ccgctgtaga tttcgcaata ggtttttgcc gcatctgcgg tacgggaaat agtgctgtag
     1021 ttggaagtcg gccgccatgt gcagttctgc gcatagccct caatggtcgt ccgtttgcag
     1081 ttcactgact tcgaaccagt ttctgcatat gagccgggat aagtcaccgt gttccagtcg
     1141 cagataacat ttcctccgtc ctcatagtag cagttttccg caaagatttt cgatgccgtg
     1201 tgtacggtat atgccatgct gaacgttttc acatagttgt tcagcgagtg gaaatagccg
     1261 tagcgcatca gccccggtcc acgggtcagc gtcttttcaa agcggttgct gataatggac
     1321 atccggggat agttgttata ggttttatag gagttctcat catctgccgg ataccccagg
     1381 atcacgccgt attcgtgaag tccaaaagag cagtccgaaa cggtggtgta gtccgcatca
     1441 tagcaacacg ccaggaattt atcccagtcg ggcaagccgg tagaggctgt gttatagtcg
     1501 ctgtgtccca caaaggtaca gtggtctacc cagagattct gacccgaacc gaggtatacc
     1561 acaatggagt cattgccgtt ggactcggca tcgtgctgta actcaaagtt tttcagaatc
     1621 agattgtttc ccgttccgct gttggaagag gtgcagaact gcacattata catggtatgc
     1681 gccgcatagc tgccgataat ggtcttattg ctgtgaacat agattcgtcc gtccggacag
     1741 tagttgcgtc cggaggagtc cttttgcagt gtggtaacac tgaggtttgc cgtaaccaca
     1801 atcgtataag gcgtatcaga agtggcgtat ttcttgaggt cagcaaaagt ggaaacctcc
     1861 acgatctcgc cgaacagacc gccgatatcc cctgccttga tgttgccggt attgtcgtca
     1921 aaacagtatc cggcaaagcc ctgcaagcca tcggcattga gcagccagag ctggttgtct
     1981 gctccggtat atttggcaag tgtcatgccg gaagttccct gtgtcaaagc aagggaagtg
     2041 tcggaatagt tcacgatctt atagtacaga tcattgccca gatgatcctg tgccacaggg
     2101 acaacgtacc aatgctggga ctgggcggat tcactgccat acacgatcac atcggttcca
     2161 gcagatacgt tgtaattgcg tggggtcaaa agccggccgg actgtgcatt caccagcttg
     2221 aaaaaggtgc cgtttgcgtc ctttcccaca cggtcaaacc gccacgatgc agacaggtca
     2281 ctgccaagcg tttgcaggga aagtgctgca ccgtcagacg ttccgttttc cgtcagaacc
     2341 ttggcattgt ccttggcggc aaggtgcatc agctgcgccg gataatccgt agatatcgcc
     2401 gctgccgaca agactgcccc gtcccaccga aacaacggca gtatccaggc tgtcagcaga
     2461 accagcgaca ggagcagcgc ctgccgcaca cgaacccaat gtttcatttt ttcatacctc
     2521 cttaaaattc ggcatagact tcaaaagaat atgccagcaa cacggataaa actatcaacc
     2581 gcattatact acattttatt gaaattgtca agatttagag atgaaaaaac cactttcgaa
     2641 aacgaagtgg cttttcctgt tatctcttaa aaattgttcc aatatctgca tccatacaaa
     2701 ttgcctgcgg tctgatctgc tccggacaaa acgcctcacc gaaattcaag caggcgtaca
     2761 ctgctttcgg gttcttcgcc gtcatctgcc agaatggata cttgataatc gccggtgtgt
     2821 tgtaccctac gccgagttcc agaaacaaaa tatgcagatt ttcgtgtctg cggagaaatt
     2881 cctcataccg ctctgccgca cgataccatc cctcgtcctg cacaaaggtt tcatctgcac
     2941 gaagattcat ggtcatgggt ctgccgcatt tgggacagcg tggaactaat tctgttggaa
     3001 taagcatatt ttcctgctca gcgtacattt tgcggataat atcctcattg tcatagacct
     3061 tctgacagca aggctctgaa cattgaaaca gtccgtaatc accctgcgtg tagaaaagcc
     3121 tgtgcttgtc aaattcggct ttctgaaact ggtggtctac attggtggtc agcacaaagt
     3181 aatctttgtc tttcacaagt tcaaacaatc ccttgtaaac accgttgtca acgtccatgt
     3241 aacggttgat ataaacgtat ctcgaccagt acgcccaatg ttcttccaga gattcaaatg
     3301 gatagaaacc gccggaatac atatcccgaa agttgtattt ttcaatgaag tccgagaaat
     3361 atttttcaaa gcgttcgccg ctgtatgtaa aacctgccgc agtggaaagc cctgcacctg
     3421 caccaatgac aatggcatcg gcggtttcta tctcattttt cagacggctt atctcaaccg
     3481 agaagtccgc tgtatatttc gtaatctttc tctgtaaaaa cattgaatat cacctctata
     3541 tcattgtcct tcaaaaattc tctcactgtt tgcacagcaa tttcagccgc cttatcctgc
     3601 ggaaaaccga acacacctgt ggaaatacag cagaaagcga tgctcttcac gccgctctgc
     3661 accgcaagtt ccagacagct ctgatagcag cttttcagca gttcgcagtg cttttcggtc
     3721 agcctgccct gcacgattgg tccgacggtg tgtatcacat agtcgcatgg cagattgtag
     3781 gaaggcgtga ttttcgctgt acctgtcggt tcttcatgtc cttgcaattc catcatctct
     3841 gcgcatttca accgcaggcg aacccctgca aaggtatgga tacagttatc aatacaatta
     3901 tgacaaggat gatagcatcc cgtcattccc gaattcgcag cattgacaat tgcaccgcat
     3961 tgcagtgtgg tgatatcgcc tttccagaga tagatatgct cctcaacggg ctgcaaatcg
     4021 gcaacacttg tgatgccttt ctgtgccgtc aatcctcgca gaaattcgtc ctcggctttg
     4081 agatactctg cacnnnnnnn nnnacaagac tgcggtaaag atggaactgc tccgattcat
     4141 ttgttggaaa gaccaaatca gtccattccg aatgttctga aagcaaatat gtagtcaagt
     4201 attttaattt ctcattttcc atgatctttt ccctctttac aatcgctccc acaggacatt
     4261 tttcaaggca attgccgcag tgcaggcagt tgttctgccg aatcactgca ctgccctttg
     4321 ccaggtcgat gcagttttgg ggacagactg tcagacacac tccgcatcca cagcattctg
     4381 cgccgacaaa atagccgttt tgtactgctt tcgtttctcc aaaggtaaag ctgtcacgct
     4441 cgatcggctt ttttgacaga tcaaaccatt ccccttttcc agcatagatc tgaaaaacgg
     4501 tcagagccgc agcactttcc gcagtggggt aaatttcgtg catatacgga ttcttccgga
     4561 acagctgcag cagccgttcc gcaccgacct cagttacctt tccgccaatg gaaagcgcag
     4621 tgctggacat ggtatcttcg cctttcacgc ctgtcaaagc aatgtatttc tgccgcttta
     4681 ggcgatgata aaaattcttg ccctttgccg tgagaaaata cagcccattt tcatcccaat
     4741 ccatgatatc aatggcacag gtgaccggca agccgttttc atcgacagtc gctgcgatca
     4801 cagaatggat ctcatccacc aaaaatgcaa agtaatctgt atatttcatc gaaattcctc
     4861 cacacgaaca ggggctgccc gttttttcgg acagtccctg tgttaaaaat gttctttgaa
     4921 cgaaatcaag caaagaaaaa tcctgcggtc atatccagat agtttccgcc gctgtagttc
     4981 ggatactgtt tctctacctc tgccttgaag tctgccgcat cactgcactt ctccgcaatg
     5041 cccttcagcg tttccagata gtcgatcttg gtctgcgcat ccttcagatc ttccggcgtg
     5101 tagtgggaag tcagaatcag atcatagccc tttgcgatat aatcacgcag ctgtgcaatg
     5161 atagcgtcag cgtgacctgc tccggcaaca atagagtggc agtcgtgacc gagcatatgg
     5221 gtgtaaactg cattgatctc cggtatctcc acatcaaatg cctctgcggt ctgatgaatg
     5281 acaaaatcaa tgccgccgat gttgattttt ccctcgccga tcacgttggt gatctggtgt
     5341 acggagttat caaaaattgc tccgaatgcg tttgtgaaat tggtgataag tgccttgccg
     5401 ccgccgtttt cggagtattc cacagcgttc tgtgtggcat acttcggcac atccggcagg
     5461 aacgctgcac cggcaccatg gtaggcaacc agcattcctg caacctccac accgttcaga
     5521 taggctgtca gttcctcgat attatcgaaa aagcagggcg attccagaat gactgccttg
     5581 ccggctttct ccacaatgaa aacctcattg tcgataaaat cgttggtttt gtaagcgtgc
     5641 agcttaacag ttccaaaatc gtagacgttc acttcaccct tggaaagctt aactgcggta
     5701 aatgtattct tattcatgat aaattcctcc tgaaagtttt ttggattgtt tttcagaaga
     5761 acaagcgctt gatctctttc tgtgattcta tgataccaca tattcctgca cttgaaaagt
     5821 acgcactttt ttgtaaggta gttaccagga ggtaactctt gcggaaaatc ggtctttttt
     5881 ccgtgtaaat ttatttgtaa ataatttatg aatcctaaaa atgttgcaat tttcggtgca
     5941 atatgctata ataaatacag tattttacaa gaaatcattg aggtgatcgt atgctgaaaa
     6001 aagaagaact gccggattgc cctgtggcaa ccacagtaca gctcatcggc aacaagtgga
     6061 agctgctgat cccgcgtaat ctgcgaatgc gtccgtggcg ttttaacgaa ttgcaaaaat
     6121 ctctggaggg gattagtcag aaagtgctga cagaaagcct gcgttccatg gaagcagatg
     6181 ggatcattgt ccgcacggtt tatgcagaag ttccgccgag agtggagtat tccctcagcg
     6241 aactggggga aaccatgcgt ccgatcctgg atgcgatgga acagtggggc aataactaca
     6301 agtcgacgtt tcattgagaa aatgcctggg gtgatatccc cggcttttta gcggaaaaca
     6361 ggttgaaaga atcgcttttt atagtgggaa aatagggatt tacggaggcg ggcatgaaaa
     6421 ataagatcct atattgcatc aattttattt ggacaagttt cgttgcattt tcatttccga
     6481 tatgctttgc ttggatctac ctggacataa ccggtcattc aaagggctat ggctatgatt
     6541 tagggtcaga aaaagatatt tctattatgg tcggctgtgt tgaacttctc ttttggcttg
     6601 cgcttgcatt accctcaaac atttatatct ttcggaaaac attaaagaaa ggcaaggcat
     6661 acttgctgat acctattgca ttgtacatta tccttgctgt gatttgcttg cgaatcacat
     6721 ttggcggatg gtccgcatac gcaaaagaag tgtttaactt ataagcaacg ctgtgtgtta
     6781 tgctaaaaag atgtgcagca ctacgataaa agaaaacgag ggagatacca tatcggtatc
     6841 tccctctata ttttcacagt gcctgccgat caaaaaacaa gcaagccaat atgatataca
     6901 acgcaggcaa tcagcagtgc cgcaagtgta tagaacacgc cgattttgaa tgtccatttt
     6961 acggagtggg tttctcttgc cgttgcagcc agagcgctga cgcagggcat attaaagcta
     7021 atggcgaata tgaaggcaag tccctctgct ttggtgaaat tggcagacag aatttcggaa
     7081 atgccggttg cggcagtacc ggaaacctta gccccaaacg tggagcttac cagacttccg
     7141 cctgcgccat agagggtatt cagaacaccg agaagggatt ctttggagat cgccgacgca
     7201 cagaatgcca gaaacgcctg ccatttcaga ccgaagaaca tggttaccgg ttcgatccag
     7261 acaccgattt tgcaaaggat gctgttttct gcattgccgc cgaagccata ggtcagcacg
     7321 aaaaagacga tggataccag cgaaatgaca cgcagcgcac gcaggaaaat atcaagggta
     7381 cgctgaaatg tgacatacat cgtattgcgg aacgccggct tatgatatgg tggaagttcc
     7441 ataatgagtc ccacacgttc ctttttgggt gcgagagaat tgccgaaaat tttatagacg
     7501 atccacatca tcaggaacat gaacaggaag atcgacacaa tgaccaggaa tcctccggtg
     7561 ctgccgaaca gtgcaatggc aatcgtaggc actacggaaa gcgtcgccgc acagggtact
     7621 gcccagctca tggcaattgc caacacacgc tgtccccagt tgtctaccac acgagttccg
     7681 gttgtacctg caatggtaca gcccagcccc atgaaaaatg gcataatggt tttgccctgc
     7741 aagcctacct tggacatggt gtggtcaaac aaaaaggaga tccgggcaag atatcctgtt
     7801 tcttccatca gattaaagcc cagcgtgatg cccagcacaa acgatgccat catcaatgca
     7861 aaatacagca cattgaccaa tacatcgcag ataagagaaa tgagcagcgg atgaacggaa
     7921 atgtgttcca ttccggtgcg cagaacgccg ctgagcgatg tcagcaccgc cgatgcgata
     7981 cctccaacga caccggcaaa gagcattgcc agaagaaaaa tcaccaggat gatgccgaat
     8041 gccatcagct tgccacgcac cggcgaaagt gccagacggt caaacttact gaacgcttga
     8101 tctgcacgtt tctgtgcggt cacaacccca tccagcagcg catcaatata agccatcttt
     8161 tcttcggcag agttcatggt ctcccctaca atgacattct tctgcgccag agccgcttgt
     8221 atggtttcgt aaagagaacg gtagtccttc ttacgaatgg cagacatagg aactaccggg
     8281 atccccagtt ttcgggacaa ttcatccgtg ttgacggtaa cgccctgtcc ctttgccacg
     8341 tccatcatgt tgagtaccag aaccgcagga actttcgttc cgacaaaatc tgccagcata
     8401 tagagactgc gcctaagctg cgaggcatcc gccatcacaa gcaccagatc ggcatttccg
     8461 gcggtgatat aatcccgggt gataacttct tcatctgaac cggcagagag cgaatagctg
     8521 cccggcaggt ctgccaggat cattcgctga ccattccacc ggcattcccc ttccttgcgg
     8581 tctaccgtct tgccgggcca gtttcccacg tgctggtgcg aaccggtcat catattaaaa
     8641 atggtagact ttccggagtt gggctgtccc aacagggcaa ttaccggcgt tttgtcatcc
     8701 tttcgataga aacggctgct gtccttcggg tgacacgttt tctcagacgc acaggcttca
     8761 caacgactca ttgcgcagca cctcccacca tgattttttt gctttcttcc cgtccgatcg
     8821 caatcaatgt atcccgtgca aagagcagca cgggctgttt cttatgattt tgacatattt
     8881 caatcatgct gccgcagagc aaccccatcg atgacagacg attgaccaga cggctttctt
     8941 ctttgatctc caaaatgcaa taccggctgc cgggggttgc atcggaaagt gtgactgcat
     9001 tcataacatt gtctcctaat ccaaataata tgcagttagt aaaatctaac cagctgcaat
     9061 tatagcagaa attccgggaa ttgtaaaatc cattcgtgta ttttaagccc gtttagcagg
     9121 aaaatttgtt ttatctcttg cgattcggca ctgtttgctg gtataataaa actatggata
     9181 caaaaaatca gcacttcacg attcaaaaag aaaatgaagg tctactgcac ttctatacac
     9241 tggcacccgg tatccgcatt tcgttcaacg agatccacac aagcagctgg gaaaaaggag
     9301 acagctctct ctacggcgaa cgaatgctga ttctgaattt ctgcgttcac ggtcggtgcg
     9361 acgcctctct tgcgcagaac cgctacgcca ttgtgaaaga aaatcaagtc tgcctcagca
     9421 ccattctgcc aacaaaggat ttctattatc ccggaaggct gtatgagggc atccagttct
     9481 atctggatct ggacattctg catcaggaaa acggacagga ctttctctca caaatgggcg
     9541 ttccgccgga gcagaccgca gcaatgttct gcgaaaagaa cgggatctat ctgcatcgga
     9601 tgaacgatgc actgctcgca ctagtcagag aggcatgggc agggaaagat gaaccggagc
     9661 tggggatgct gcggtatttc acggtacgtc tcctgcacga actgctgtct atgcccaccg
     9721 aaagcgaacc ggatatctat ttcacacggt cacaaattgc aattgtcaaa gatgctgagg
     9781 ctttgatttt aagcgacttg agcaagcgga tcaccgcaaa ggaactggca gaccgattcg
     9841 gcgtcagtga aagcagtttc aagttctacg taaaaggaat cctcggtgaa agctatctca
     9901 gctatttcag aaagaaacgc atggaaaaag cagcggaact tctggaatct acaacaatga
     9961 aggtcattga ggttgccaat gcagtgggct atgagaacca aggcaaattc gccaaggtat
    10021 ttgcagaaac atacggtgtg ccgccgctgg agttccgacg gctgtcgaag tgaatgccgt
    10081 tttccttaga gctttatcgg aagttttcgt agcagatgcc ccggtttgtc tgagatactg
    10141 cgctgcacaa ggaatgcacc aaaaataatt tctctctgat ttgcattttc cgcaattttg
    10201 tgttatacta tttttcatca tatccttgaa aggagcctgc aaatgtgtac agctgcaact
    10261 tatcagacca gtgacttcta ttttggcaga accttagatt atgaaagttc ttacggcgag
    10321 gagatcgtca ttcttccacg aaagtacaag ctgccgtttc tgcatatggg cgtatcagaa
    10381 cagcactatg caatcatcgg aacagcacac atcgccaatg gatatccgct tttttacgat
    10441 gccgtcaacg aaaaaggact ttgtatggca gggctgaact ttgtgggaaa cgcctgctat
    10501 gctgcccgga ctgcggatga ttcggacaat gtggcacaat acgaatttat cccatggatt
    10561 ctcagccagt gcgccgacac agccgaggca agagcgttta tccaacggct caatctcctg
    10621 gacacgccgt tccatgcgca gatgccggcg gcgcagctgc actggatcat cgcagacaaa
    10681 aaccaaacca ttacggtgga atccatggca gacggaattc atgtttatga aaaccctgcc
    10741 ggcgtgctga cgaacaatcc gccttttccg gagcagatgt tccggctcaa cgactatatg
    10801 cacctttccc cgaaacagcc ccaaaaccat ttttcaacgg agcttccgct gagatcctac
    10861 agcaggggaa tgggcgcact gggtctcccc ggcgaccttt cttcccagtc ccgctttgtg
    10921 cgtgccgctt ttgtccgggc aaattctgtt tccggcaatt ctgaaacgga aagcgtcagc
    10981 cagtttttcc acattctcgg tgcagtggat cagcagcgtg gctgctgcga agtcaaagac
    11041 ggacagtatg agattaccat ttacacctcc tgctgcaatg cagataaggg catctattac
    11101 tacaccacct ataacaatca ccagatctct gcggtccata tgcaccacga aaacctggac
    11161 agcagtcagc ttgtctgctg tcccatgcag acaaaggaac agatctgcca ccagaactga
    11221 aaggaggcgg cacaccgcat atgtcaaagg taaccaaacg tgcgctggag cagtcactga
    11281 aaaatctgct gctgaaaaag ccgctgacca aaatcaccat cggggacatc acagaggact
    11341 gcggcatcaa ccgcatgact ttttactatc acttcaaaga tatttacgat cttgtggaat
    11401 gggcgtgcct ggaggatgca cggcgtgcgc tggatgagaa aaagacttat gacacctggc
    11461 agcagggact tttgcagatc tttgcggcag tccaggaaaa caaaccgttt atcttgaatg
    11521 tctaccgctg tgtccatcgg gaacaggtgg agaaatatct tcagccgctg gtggatcagc
    11581 tgctgctgga cgttattgaa gaagaatctt ccggcatgat gatccgggaa gaggacaagc
    11641 agttcatcgc caaggtctac gcctatattt ttattggcat catgctggac tggatcaagg
    11701 atgatatgcg ggaatcgccg caggaaattg tagtacggct tgggaaactg ctgcaaggtt
    11761 cgatctctgc ggcaattgca agattccggc tttgagcatt gccgctttag cgctgttttt
    11821 atcattgctg catttttgat ggattttttt acactttccg gctctgtgat aaatttttta
    11881 tcacagagcc ttttttgctg attgtggatt ctgaaaattc gggtataata gaatcataag
    11941 aacacaacca gcataaaacg gatatcgttt tatgaaaatc caatttcaag ctctgacgta
    12001 aggaggcatc gttatgtatt attccgcagg aacttatgaa tcatttgcac accccgaaaa
    12061 gccgaaggac gtggacaaaa agtcggctta tatcatcggt accggtctcg ccggtcttac
    12121 cgcagccttt tatctggttc gtgacggtca gatgaagggc gaacacattc acttgctgga
    12181 aaagctggaa cttgccggcg gcagctgtga cggacgcaag gacatcacaa aaggattctt
    12241 catgcgtggc ggcagagaaa tggacaacca ttttgaagtc atgtgggata cattccggga
    12301 tgttccttcc atcgaaacgc ccaacgtctc tgtgctggac gaatattact ggctgaacaa
    12361 gcacgacccc aactattccc tctgccgtgc ctccatggac cgtggcaaag atgcgcacac
    12421 cgacaagaag tttgcgctgg acaaggaatc ggcgctggcg ctttccaaac tgtttctgac
    12481 accggaaaaa gatctggagg acaagaagat ctccgacgtt ctgccggatt cgttctggga
    12541 gaccaacttc tggctgtact ggcagactat gtttgcattc cagcgctggt ccagtgcatt
    12601 ggaaatgaaa cggtatctgt gccgctatgt ccaccatatc gacggactgc cggacttcag
    12661 cgcactgcgt ttcaccaaat acaatcagta tgagagcatg atcctgccgc tggtaaaata
    12721 tctggagtcc cacggggtcc ggatcgaata cggcatggat gtcagaaatg tgattattga
    12781 aacggtcggc agcaagaagg ttgccaaaca gatcgtatac cgcaaggacg gcagagaaca
    12841 gaccattgat ctgatcgagg acgatctggt ctttatcacc aacggctgct gtacagacac
    12901 ttcctgctac ggcgaccaga cccatgcacc ggatctgtct catctgaaaa acggatacgg
    12961 ggaatcgtgg gatctgtgga aagccattgc atcacaggca gaacacggcg aatacggcaa
    13021 accagaagtc ttttgcagcg atattgatgc cacaaactgg atgagtgcaa ctgtcgccac
    13081 ctcggacgag gagatcatcc agcacattat cgacgtctgc aagcgtgacc cacgcaccgg
    13141 aaaagttacg accggcggta tcgtcaccgt caaggacagc acagagaact ggtatctctc
    13201 ctggaccatc aaccgccagc cgcagttcaa atcccaggac aaaaacatgg ttctggtatg
    13261 gctgtattct ctgaacacca acaagccggg aaactatgtg aaaaaagcca tgcggaactg
    13321 caccggcgaa gaggtctgcc gggaatggct ctatcacatc ggcgtaccgg aagagaaaat
    13381 tgacgatctg gcaaagaacg cctgcaatac gaccacctgc tttatgccgt atatcaatgc
    13441 gtttttccag ccgagaaaag aatctgatcg tccccgtgtc gttccggacg gcgccgttaa
    13501 ctttgccttt atcggacagt ttgccgaaac acctcgtgac acgatcttca ccacggaata
    13561 ttccatgcgt accggtatgg aatccgttta caccctgctg gacattgacc gtggcgtacc
    13621 ggaagtctgg ggcagcaagt acgatgtacg ggaactgctg cgtgcctgct attacgccat
    13681 cgacaaaaaa ccgctcacag acgaaaatct ttcctttgtg gaaaagaaac tgctaaaaat
    13741 cgtcttgaaa aaagtcaagg gaacggatat tgaaatactg ctgaaaaaca gcggcttgat
    13801 cgaataagtc tgttcgctgc actcgaaaat gaaccagccc ggaattccag ttacagaatt
    13861 ccgggctggt tttgtttgta tgcagtgaaa ccggatattg cagattgaca gctgttcccg
    13921 attccattat aataaagata atcagaatcg taaaacagga gattttcata atggaaaaaa
    13981 cgccatttcc aaacagcttt gatcttatcg cactatacgg cagaaataca gattattccg
    14041 acgagagcga tgattgcaga atataccatc tgaacaatga aaccggcgca ggaacgatca
    14101 cgatctatca cgtttttcag ggcatccagg ctgcgttcaa cgatattcac atggcgtact
    14161 gcaatttaca gcagaacact gcatcaaaca tgatcgagat caaccactgc acagaaggta
    14221 gatttgagtg cgatgccggc gaacagctgt gctgctacat ggcacccgga gatatggctg
    14281 tcaaaagttc ctgtacctgt tccgaaaatg cctgctttcc tacaaggcat tatcacggaa
    14341 tcagcatctt tatagaaccc gaccgctttt ctgaggaact tctcgccatt ctgaaaatgc
    14401 tgtctgtgga ttttgataag atccacgcaa tggtatccaa ccaaaaccag atcctaatcc
    14461 tgcgaaaaaa tgaaaccgct tcccatattt tttcggaact atacaccgtc agagagacgc
    14521 accgggcagg ttatctcaag ctaaaagtgc tggaattgct cttgtttctg acggatacga
    14581 aatgcacagt gtccaatcag acggaacact acttcgaccg ccaaagcgtc tctctgatca
    14641 aatccattca tgactttatg atctccgata ttcaaaagca ctataccatt ccggaacttg
    14701 cagaaagatt ccgcattgcg ccgacaacgc tgaagaaaaa cttttcggac atctacggct
    14761 cttccgtata tgcgtacctc aaaacctacc gcatccagga atcgcagaag atgctgctcc
    14821 gcacggagct ttccattgcg gaaatcgcca atgcggtagg ctatgagaat ccggcaaagt
    14881 tttccgcagc cttccagaaa gaatgcggca tgacaccctc tgcattccgg aaatatgtca
    14941 gaaaccgtgt ggagcatgac tgacttgtcc gtttggatag aaattgtcca aacaggtatt
    15001 gcagctgcca gttctccatg gtataatttc atgtgttagt tttcactaac taagtgagag
    15061 aaaaaggaga agatcacatg gatttcaaaa agttattatg tgcggctgca tccctggcga
    15121 tggcagcatc tatttttacc ggctgcggaa aaagcgaaga gagttcggtc tccaaggctg
    15181 aaaactctgc cgcatctgag agcgcatcgg atgaaaacgc agaggctgcg gagaaactgc
    15241 tcaccgatct gaacggcact tatcgtgaac tgtggccggt tattctggca gacgactacc
    15301 gccagacttg gctggatgac agcgcaaagc tggtcggcga ggaaaacgcc gaaggtgcat
    15361 ttgaaaagat gtcctctatg gtgaccggta ctgtgatcgg cgaagatgcc gtaaagaaat
    15421 atgcggacgg aaacatggcg tactgctgcg acttcacaca ggatgtagac acattcacgt
    15481 ttgatggttc caccatttcc ggcaaggatg cagacggcag cgaactgttc gaacatacct
    15541 atcactatat cggcatggag gacatcagag ggctgtacat ctatgaaagc gatgatgcag
    15601 attccggcga gttcacctac ttctgcatgg ctcccgacac catggcgacc acatggcaca
    15661 tcgaattccg ctacggcagc gatctggatg cactgaaaca gtacgacgcc ggtgaatatg
    15721 cgtactggct ggcatcgggc atttctgtgg attacactgc ggatgatgtc agcaactgca
    15781 ttgaactttt ctgtacggag aatctttcgg agtgataatg tcctagcatt ccgaacaaaa
    15841 aataccatac cgggaaaacg attgtttccg gtatggtatt tttttggttt gtatttgaag
    15901 tgccgagtca tatctgttcc agatccctgt atgcttctag cgcaggactt ttatcaccca
    15961 cgtaccagta gtcacagcaa tcgccgccaa gcgcctctgt ccgggtacgg atcagacggg
    16021 cgtgcatgac ctcagcgaga tagtggtctg tcctgcaaag gtacggcaag agttcttcgt
    16081 agccgtgttc ctttgcgtgt ttggcaatcg gacaaccgat aaggtgaaag caaaagccct
    16141 cggtgcggtg gtcggggttg attacaacac gccagctgtc catccattcg ccccgtgcct
    16201 ggtgtgcctt ctgcattttg ttatatttca catatgtctt ttcaaacagt ttatacggcc
    16261 acttctgccg gttgccgtca acaaattttc cgaggaattt cattttgtct acggcacggc
    16321 gtttgatctc aagcagcgcc tcgccgtcaa gtttgtgttc gctcgcctca taaaatgcaa
    16381 ggatcgtaaa ccaatccagc atattggctg ccatcatatt tttccccaga tcgggcatat
    16441 ctttcataaa tgcaaggtag cattcttcgg ctttcgcaaa aatctgctca gcatcatccg
    16501 ggtatttttc ccggcaatat tgctcatatg gctttgcata tgatgacttc gcataggctc
    16561 ttttcatggc agtatcccct gctttctttc aaaaaatcaa cgtaatgatc ccgcccacaa
    16621 ctgctgctcc ggcaaaccag atgagcgcca tgatgctcct ttttatcaac acctgccgcc
    16681 aggtctgcac aaacggcata tcttccagcc ccggcagcac ccagaaccgg ctgtgtccca
    16741 cccatagctt gtcgatcaca acgccgtcat aaatattcat cacttcgaga aacagcagtg
    16801 cctgccagta cgctgtgcca aaagcagaaa caccattcca cacaccaata atcaatacaa
    16861 gtgcaataaa catcaccagg cagaacaccg tcatgaacca ttttcgtttt cggctcacag
    16921 tctcccggtc ggtcattccc aatgcaatgg cacgatcctg tacctctttg ggatagaaat
    16981 acagcccatt gatcgctccg ccgattacga aaagctttac cattacggtg aacagcaaac
    17041 aatagagcag caattgcagg ataatcatgg tcttttcctc ctctgtttgt gtcccgaaat
    17101 gtgcttattc gctcatttcg cttagaaaat tgcgaatcgt tcttacataa tcgtctgctt
    17161 tcagcacggt ggaaagatgc ccgccggaaa catagacgat tttcggatcg tgcatcagct
    17221 tgataagatc ttcctgcatt tttccggaaa aaaagtcctg attcggcagg atcagaagaa
    17281 ttttaccctg gaccgccgca aaatctgacg ccgtcacagg aatctgattc atcaggtcgg
    17341 cgagaagtcc tgagatatgt acatcctttt cctgctgata atcccggaag atcgtatcga
    17401 acatatcctg cgcataggcg acctcttctg cgctttcgtt gcggatgtgg ctcattccca
    17461 gcttaataag tttcggtttc atcttctcat aattgcagcg tttcagatac cacagcatca
    17521 gcagcgcaaa acggtacttt cttttcagac tcttgagtgt attggcatcc atgccgcctg
    17581 ttgaaatcaa gatcagaccg ccgacctcat cgggatactt ttgcgtgtag atctgtgcga
    17641 ccatgccgcc gtcgcttgca ccaataaaga cgggatctta gatgccgaac ttctgaaaga
    17701 acgcgtgcat tccaacaacc agttcctgat tggtgcgcag ttcctgggga tagtcaaaca
    17761 gaagaacacg gcagacgtca gacagtccgt ccacgtaatc catccacatt cccagcgtat
    17821 tcattccgcc gtttaagaat acgagtgttt tcccatccgt ttttccgctc agaatatagc
    17881 gaaactgtgc gccgtcaacg gtgacgttct cgcagggatg cgctgttgta aacgattgaa
    17941 ttttttcgga atataatagc attgcttttc tccttttatt ttgtatttct gcatttttct
    18001 tccggttcct aggggttgtt tgaaaaatag cgatgtttgg aaaaatgcat agaaattact
    18061 gtatgttagg agagaatccg caggcaggct atcgcctggc aaggattttc gacgacacag
    18121 acagtgattt atgggcgttt tgacaaatgt acggattttt caaacaaccc ctaagaatcc
    18181 ggatatttta tcagatcata aaggacgccg tattcgccaa aacggcgtgt accggcaagt
    18241 tccataccga ggtggatata cttatcacac ttggagcgtg cgtcggtatc aaggatcacc
    18301 ggcacgccga gtctgttgcc ctcagaaaat gccatatcca tcactttccg catatacccc
    18361 tgtccctggt atggctctct cacacaaacc agccccacat agatatatgg tttctttgct
    18421 ttgtcaagct gtttgcccag ccccgaaccg ccttttgaca tgatacgtgt gaaacgaagc
    18481 agttctctga aattcataga gccgagcaag ccctttgcca gaggcagtac agctttcaga
    18541 ctggcatttt tctgcccggg aagtttgtac gcaatatatc cctcgccgtt ttcacccacg
    18601 gtgtagagca ttccgctttg cagtgccatg cgcacataac cgcagataaa tgatgcaaca
    18661 gcgtcccggc ttgggaatgc gtcgatcaat ccatgttccg tgccgtagtc atagtagccg
    18721 aaaacgtgtc caatgtccct aataatactt tcctctaatt ctgttacctt cttcattccg
    18781 ctttttcctt ttcttctttt tgcaggactg ccgctttatt ttttcaggat ttccgttatc
    18841 ttggcaaaat cctcttttgc ctccttgaaa taagaacttc gtctttcgca ccggatagct
    18901 tatagatact gcgcagcaac gatgcgaaaa tgctcatatg gatttcccct tttcaagaac
    18961 agcgatcttg ttggaaatat tgcgcacaaa cggaattttt ccaagcagct tatagatggg
    19021 cataaacacc gccatgcctt cggtcagagc gtgttcttcg acaaattgga aatccggcaa
    19081 gagtgctgcg agcgccttgc cgtcctttat gccccatgtg aatttcgcac ggctgccctc
    19141 gatggatttt tccttgaatc gttttgcaac agccggattc atgatctcaa ccagcacagc
    19201 agctttcggg aatcgcttgg aaatcactgc aaaaatccgc ttgacatctg cctcagacag
    19261 gtacatggtc agtccctcaa tgatgaccag tgccggcaca tcctgttctg caattttgct
    19321 gccccaatcg tccatggcag acatagcaat ctgtgagatc ataccgcttt ccggcagcag
    19381 tttttcccga acagccatgg tctgcggcaa gtccaggttg taccagtaag aaaacccacg
    19441 catccgatag catcgggtat ccagtccgca ggctatgttg actaccactg caccggaatt
    19501 tttggcgagc cacgccccca ccaaccgatc gagtaccagt gtgcgtgcaa tcacaccgct
    19561 gcgcatagca gtatccttgt ctgcgaggga aaaatcgtag tccagttcgt cgatgatctc
    19621 caacgctttt ttgtcttgaa tgacaccacg gcctgtgctt tctttggcac gggcgtatac
    19681 cgtttgcagc atcgtttccg gcacaccgga aagttttatc ttttcagcca tatctgttca
    19741 gcactcctct tccattttcg ctagttagaa atcgctaacc aaggaaacct aaaaaagctg
    19801 cctaacggca gcttttgctt tttatggatt gttaaagcgt ccaaaagcat cgttttcccg
    19861 ttttatggat ttctgttatt tctattatat cacagctttc gtggaattac aatagaaaaa
    19921 tgaaagatac gggttttaca gtcttttgag agacactttt gcataccggc atacggtaca
    19981 ttctaaagcc ttgtccgctt tccgatgctg catccttccg gcgtaaaaac acaccataag
    20041 cgattttggg atgagcggaa cagtttcatc ccaacttgga acagctgttt tgagaagcac
    20101 agattcgctt agatgctgca tcatttcaaa ccggaaggat acagcatcta tttttacaga
    20161 tgctgtatgc ctgtttgtat ttgaagtttt ccgaccttac tgtttggcgt tttcttcctg
    20221 tttcagcagt gcataacgca tctggcaaag gtcaaatcca ttcaaagtct gatcttcgtt
    20281 gaaatctgcc gctttccagt ctgcaagctg tgtatccggt acagccagca gccacttttg
    20341 cagtagcacc acatcagaaa ttgtcaatgc accgtctgca ttaacgtctc caaggattcg
    20401 ctcttgatac tgaatgttag ctcctgtcag cttttcattc agcaaaccaa tggtaatctg
    20461 attccactct tcttctgttc cgttatagga aactgagcgc agatttttgc agttaaaaaa
    20521 tgcggaatct cccaccttaa tcaaggaatt cggaattgca atggtctcca gataaggatt
    20581 ttcggaaaaa gcgtacattg caacagatgt aacaccgcct ggaatggtat atgcagcatc
    20641 tgtctttgcc gccggataga ttaccagtgt gtcctgcgcc ttggagtaca gaacaccatc
    20701 taagctggaa aagttggggt tatctttcgc aacctggact tcccgcaaag cactgcagga
    20761 accaaacgca tttttatcaa tttttgcaac tccagttgaa agtttcacag atttcaaaga
    20821 agtacagccc aaaaacgcca tttcgccaat ggaagttact gtattcggga tttcgatgga
    20881 tgtaattgta gagagataaa atgctccgac agagattgca gtaactggaa gtccttcgat
    20941 ttcagaggga attacaacat cggaaacgga attgttaaag ccggtgatct caattgaatc
    21001 tccatggttg acataggtga atgcggaact gtctgtagcg tccgtattgg gaattgtata
    21061 agcataatat acatagttca gtccaatata gtccttgaat tcaaactgat aagttacatt
    21121 attataccga atggaatcgg catctaccag tgcataaccc aacgtttcat ccacttctgc
    21181 cagggagttg atcctgtcgg atagagcctt tacctttttc tccacagaca atgctgcata
    21241 ctcttctgaa ttgatcaatg cattgagtgt atccgtaatt ttctgcatat ccgccagttc
    21301 ctgctctgtg aattggcgat agacacggac tgtaacagta tcggaagtat aggtctttcc
    21361 attcactgta tactgtgcat aataagaata atcccctacc tgatttccgg acagatccag
    21421 ataattggag tacacgccat cattttcgta caagtctccg tcatttggat aggtggctgt
    21481 gccatcatca taaagcattg ccacttcctg atttgtagag gcatctatca gcgaaatgga
    21541 atctacattc gcagctgttt cagccgtaaa caaaacagac atttcttctg aatctacctg
    21601 aagccgtttg gcactggaac tcagtgtaac atcagtcgca ataccaggag atacttgatc
    21661 agggtctttg accgcaatgt ccagataaca cgtcacatca gaaaatccgc caacaccctc
    21721 acattgatag gcatagcaat gcttttcctc atcatagttc acagagtttt cttttacata
    21781 tccctctgca atcagctgct cgaacagtgc atctgccatc tgctttcggg tttcctgatc
    21841 tgcattctga aatgtggtat cgctggacag tacacctacc ttttgtgcga tttctgccat
    21901 tctttcagca tcagcatctg ataccgcatc ataaattaca acagaaaagg tttttgtggt
    21961 agaaactgtc ccatcagcat ctgtcaggcg agcataataa gaataagaac caactgccgc
    22021 attctcaaac gacagcacag cagaccaggt tctgtcattt tccaggtcat ctccgctaac
    22081 gtcgaagttg ccgtcatcca gcatttttgc gactacttcg tttgttttgg catcaaccag
    22141 ttctacatcg cttccggaat actttaagca agtagccgta aagatcacct ttttatcggt
    22201 ttcatcttcc ataaactctg cgtttccagt atgctgattt aaccgcagtg taaaagaagg
    22261 atactccgct gcacaaattt gctccgcacc gacaaaaaag caggtgctgc tcatcattgc
    22321 agcagtgagc atactcagaa ttcttttctt cataggacct tcctcctcat taaaattata
    22381 ttttattata ccataaaaag aacacccgtg cgagaagtct acgaaactta ccgtgttttc
    22441 agcgaaactt actgtttttc atactttgtt ctcatttccc aatgtgtttt ctgacattca
    22501 cagggcaggc atcatggcgc agtcctttgt gaatatccta gccgaatttt cgcaaaaagt
    22561 caacccgatt tgcgtagttg gtatgttttt ttgcgtaatt ggtatgtttg gcgccttttc
    22621 cgcacaaaaa atgcgatatg gactcgcccg gaatcacagt gcttttcagc acatttttcg
    22681 gtaagtcaat catatcgcat tttatcgcag aatgcaatgt tttgttcccc aaatgcaggt
    22741 tttcgtcccc caaatgcaga aaacgtacca tttcagtacc ggtgttctgc tgaatgacag
    22801 aaaacaatgc cattttcaca gctggtgctt ttatactgat tttcgcatca taatatccag
    22861 aacaaaccag ccgtcttttt cgtaatactc tgttccgccg tcatactttt ctacaaggtt
    22921 tcgaatggac tctgtgccga atccatggta cttccggttt gcctttgtcg tcagcaattt
    22981 tgcgttcttc tgcaaaacag aggaagagat cgcatttttt atctcgatcc aaaggtaatt
    23041 tctcttgttt tttatggcaa ttgcaatttc ctttctcttt ttctgctgca aggaggattc
    23101 aatggcattg tccagactgt tggcaagcag atttaccaga tcgtctgcat cgaaaccggc
    23161 aaagtcggta tcaatcagac aagaaaccaa aatgcccttt tctttcgcat agttgagttt
    23221 gatattcaag accatatcca caagatcgtt ttgggtctta acataagaac gaaagtttgt
    23281 tattttttcc cgggtcagcc gctccagcgt ggcaactgct ttctcctggt cgtcatcgag
    23341 aatcgctttt tgcacgcaaa gcagctcatt tttcatgtca tgctgtaaaa tctgcaaatt
    23401 gtgatagact gtataaagtg cgtcatatgt tttcttttgt tcttccagct gtgtctgcat
    23461 aattgtatac tgtagttctt tttggtttct ctctgccaac atcgaaagca gaggaagtac
    23521 acaaatgtta atcaagataa tcccaa
//
LOCUS       DAWENO010000011        23337 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_905_length_23337_cov_10.172408, whole genome shotgun sequence.
ACCESSION   DAWENO010000011 DAWENO010000000
VERSION     DAWENO010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23337
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_23337_cov_10.172408"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1198..1229
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4443..4452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6535..6544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18245..18254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20647..20656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5286 a         6659 c         5977 g         5343 t
ORIGIN      
        1 ggcatcagaa tgttggcaaa gctgttcgcc caggcagcgt tctgaaacag ctcttcgttc
       61 ttttggtcga aaccagcctt ggtttcttcc tcgtggcaga acaccttgat gaccttttgt
      121 ccgtggatca tctcttcaat atagccgttc accgcaccga tggagttctg ctgcttgacg
      181 aagaacgcac cgctcttgcc gcccacgaac ttggtaatca ccatcatcac tgccacaaac
      241 gccaggacaa acagcgtcag ccagatgctc agacgcacca ttgccacgaa tacggcaatc
      301 accgtcacga tggacgaaaa gatctgcggc acactctgcg ccagcatctg ccggagcgta
      361 tcggtgtcgt tggtgtacag gctcatgatg tcgccgctgg catgggtgtc gaaaaagcgg
      421 acgggcagcg tctgcatatg ggaaaacatc tcgtcacgga tggttttcag aataccctgt
      481 gccaccacga ccatgagccg gttatagaac agcgtcccca gaatgccggc ggcgtacagc
      541 acgcccagtc ttgtcagcgc tctcgccaga ccggagaagt cccggctgcc gtccagcagc
      601 atgggagaaa tgtaatcgtc gatcagcgtt tgcagaaatg tcgagcctgc cacgccggca
      661 atggcactga cgagaatgca caccagcacc agcacaagct ggagccaata ctttttcata
      721 taggaaagca gccgcacaaa gctctttcct gcgccctttg cagccccggg catcatgacg
      781 ccacgaggcg gcggtcctcc ttgtcgtttc tgactcattg cggaacactt cctttctgct
      841 gggattcata gacttcccga tagatggcat tgctttgcag cagttcttcg tgtgtgccga
      901 cgctgtccac tctgccctca tccattacga tgatcttatc ggcatccatc agcgaggtca
      961 cacgctgggc aatgatgatc ttggtggtat ccggaatctc gtcccggagc gcctggcgga
     1021 tgcaggcgtc cgtgtgggta tccacggcac tggtggagtc gtccagaatc agaatcttcg
     1081 gctttttcag cagcgcccgt gcaatgcaga gccgctgttt ctgaccgccg gacacattgg
     1141 aaccaccctg ctcaatgtag gtgtcatagc cgtccgggaa cgtctgaatg aattcatnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc caggcggcag acacgctgca tttcctcatc
     1261 ggtggcattc gggtcacccc agcggagatt ctccttgatc gtgccggaaa acagtacgtt
     1321 tttctgaagc accatagcga cttcttcccg gagtttctcc acgtcatact gccgtacatc
     1381 cacgccgcct accttcactg tgccgatggt ggcatcgtac agccgtggga tcagctgcac
     1441 cagcgaactt ttggcgctgc ccgtgccgcc cagaatgccc acggtcatgc cagccggaat
     1501 gtgcaggtcc acatcctcca gacagcattt gtcctcacgc ttgctgtagc tgaagctgac
     1561 atggtcaaag tcgatgctgc cgtccgtcac ttcggtgaca gcgtccgccg ggctgacgat
     1621 atcgctttct tcgtccagaa tttccacgat acgctcgcag gaggcacggg aaatggtaat
     1681 cataacgaat accatagaaa gcatcatcag actggacaga atctgaatgg catatgtcat
     1741 aaggctcatg agctgcccgg tggtcagtgt ccggctgacg atcattcttg cgccgaacca
     1801 ggagaccagg atcagacagg catacataca aaactgcatc agaggcatat taaacgccag
     1861 tcgcttttct gccctggaaa aatcccggta gatatcctcc gaaatgccgg agaacttctg
     1921 aatctcgtgg tcttcccgga caaaggactt taccacacgg atgccgtgga ggttttcctg
     1981 tgtcacattg ttcagcttat cataggtttt gaacacccgt tcgaaaatgg ggtgtacccg
     2041 tgtcataatg aaatacagtc ccacgcccag caccggaatt gccgccagat agatcagtga
     2101 cagctttgtg ctcagccgga acgccatgac catggagaag atcagcattg ccggtgagcg
     2161 cagcgcaatg cgaatcacca tctgataggc gttctgcacg ttggtcacgt ccgtggtcag
     2221 acgggtgacg atgctggctg cggaaaactt atcgatgttg gaaaaggaat acttctggac
     2281 acggtagaac atatcgtgcc gcagattccg tgcaaatccg gcagaggcaa acgccgcaaa
     2341 gtgtccggcg agtgcgccga agatcagaga aagcacacag cacaccagca ggatccagcc
     2401 gtagtgcatc accgcatcca tgccgcctgc gccgtcaatg ccctcgtcga tcagctttgc
     2461 catcagcagc ggtatcacca cttccatgac aacctccagc accacaaaca acggggaaag
     2521 cagggaaaac aacttgaatt cccgaatcga ctgcattagt tttcgaatca ttcgctgtga
     2581 cctcctttac aagtccattt ctgtgaaatg tcgtcgccca ggttctcccg cattttttgc
     2641 gccacccgga gaaatgtctc ctgatcctca tccgggatgc cctgtgccag catttcttca
     2701 aatccggcaa tatcctcctc gatctgccgg tgcaggtcta ctgccttttg tgtcagcacg
     2761 atccgcttga gccgggcatc ctgtgccacg ctctcccggc agatcagccc gtttttctcc
     2821 atcagctgca acgtcaccgt caccgtagaa cggctgatgg aaaacgcctt ttcgatatcc
     2881 cgctgaaaca catcccgacc ccggttctcg tacagatacc ggatcaggta gccgtgcatt
     2941 cccgtcagac ggtctgcgtc ctggtgcgct gcggaatagc cacgccgccg tgccatcatc
     3001 ttttccagcc ggtgtatctc cagtcccaaa tgccgcatcg cctgcctcct ttctgcgcct
     3061 ttccagcgcc tgttatttct gtttaacgca aaacagttta tcaccaaact gtttactgta
     3121 aaactatttt agcaaactcc ccgaaaatgt caagggtact tttgcaaagt ttacattttc
     3181 ttcaaacgat agcctatttg acaaagccag tgggaaaatg gtatactata ggtaacatga
     3241 gaaaaggagg ttttcctctt gcaaaaatac cgctattatc tgccgccccg gtcaaaactg
     3301 ccctttgcgg caggcattct cgccgggcta atgacgctga acaccatata tacgctgatc
     3361 tctatgccct actacaccca ctactgggac tactgggcaa ccatggtctc cggcatcctg
     3421 ctgtgtggtt tctgctttct gttccgaaac cggcacgccg aactgacctt gatcccggcg
     3481 gcaatgctgg cgctgattgc ctgcatcacg ccgaatctga ttcactggat ggaagtgggg
     3541 ctgttctttc tgctgctgct ggaatggctg gtgcggatgc cacgctggac aggcaaattg
     3601 ttccgagtcc tgggcgttct gttcaccctg gtcggcggca tcgccattct gtctcccatg
     3661 gcagagcgca tctcctcgct ggcggagcgt ggaaacgctg tcccggcgtt tgttgtcccg
     3721 tttgttatcc ggagtctggg cggcgacctg ttgattttgc tgacgctgct cctgctggtt
     3781 ttcgccatgc agccccatgt cctgccgggc tggatggatg agggcgatca gtacgaccgc
     3841 atctgggaat aaaggcaaca tgacacaaaa aaccgtaccc cggtgttttc caaggtacgg
     3901 tttttctatt gcaacgtttt tcttattaga ataaacgctt tattctgtat aaccggattc
     3961 tttcatgcac tgcaaaatgc cgtcacgcag tgcctccggg ctgcccattt ccacaaaggt
     4021 gatgcccggc atcccgtctg tatagcgttc ccgtgtggca gcgttgtcgc ccagaatcat
     4081 gggctttttc attgcctcat agatatacgc ctttccgggg atggtgcggt tcgccttgcc
     4141 gttttccttg ttgaaatgcc cggcgagaca gaggtctgcc tggctgatgc gctccgccag
     4201 ctccggctgg gaaagccacc ggatataggt gatattcggg ctttctgtgg gtgtgacctt
     4261 ctgatccacc ggtccgataa agtagaagtg aatccgggga tcgtctgcca gcagcgccat
     4321 tgcctgcatg ataacatcca cgccctgcac cggcagcaca ctgccgaagt acagcacgat
     4381 aaactgctcc ttcagctccg gcggtttcac ctggggacgg ggatagtaga tgctggtgtc
     4441 tgnnnnnnnn nnatagctct tctcccggct tcccagaacg gtcacacttt ttgcggtctg
     4501 ttcgatcagc cggatttcct gggtgttccg gagatagtcc aggtttttgg tggtgacgaa
     4561 cagcacattc tgattctgat aagactgcaa gatactgccc tgccttatgc gacggagttc
     4621 acaaacatct catagatgca gcgaattgcc tgttcaaagt cggtctcctt cacagcgacg
     4681 ataacgttca gctcgctgga accctggtcg atcatgcgca cgttgatgtg tgcgtgtgcc
     4741 agcgctgcga aaattcttgc ggcaacgcca cgagtccggc acataccacg gcctaccaca
     4801 gcaatcagcg caataccgtc ctctgtttcg atgctgtccg gctgtactgc cttgatgatc
     4861 tcggtgcgga tctcgtcctc cagaccgttc agatgcgcag agtctacgat aacggtcatg
     4921 gagtcgatgc tggacggcat atgctccagc ggaatctggt gttccgacag cacgttcagc
     4981 acgtcacggc agaaaccgat ctggctgttc atcagatcct tgtggatgtt gatggaggtg
     5041 aagtccttct tgccggcaat accggtgatg gtgtatgcag acagttcctc atcggtgctg
     5101 tccggcacga tcatggtacc cggctcatcc ggtgcgttgg tgttgcggat gttgatgggg
     5161 atgcctgcag aacgtaccgg gaagatcgct gcctcatgca gaaccgatgc gcccatgtag
     5221 gacagctcac gcagttcggt ataggtaatg gtacggatca cagccggatc gtctacgatg
     5281 cggggatcgg caaccaggaa accggaaaca tctgtccagt tctcgtacag ggatgccttg
     5341 actgcgccgg ctacgataga accggtgaca tcgctgccgc cacgtgaaaa agtcttgaca
     5401 tagccgtctg cgccgcagcc gtagaatccg gggataacgg cattttccag accgctcaga
     5461 cgctcacgaa gtaccttttc ggtcttttcg tcgttcaggc tgccgtccgg ctggaagaag
     5521 atgacttctg cggcatccac aaagggaaag cccaggtatg cggcgatgac cttaccgttc
     5581 agatattcgc cgcggcttgc ggcatagtcc ttgccggcac gctttttgaa gttttctgcg
     5641 atctcagaaa attctgcctg caaagaaaaa tccatgccca gaccggaaat gatgtcgttg
     5701 taccgtgcct gaatcgcatc cagggcatcc gagaaatcca gaccctgttc tgccaggtca
     5761 tagcaggtgt acagcatatc ggtcaccttg atgtcatcgg aaaaacgctt gcccggtgcg
     5821 gacggtacta cataccggcg ctccggatct gccttgatga ttgctgctgc cttctggatc
     5881 tgcttggcat ctgccagact gctgccgccg aactttacta ctttcgttgc catatgtctc
     5941 acacagagga aaaaccggaa cggtctctcc tcactcctta ctaaaatgat atttgcagcc
     6001 gatttgggag ggctgcggac tccggtgatg cgctttttct gccgcatcac atgatactat
     6061 attagtatat tgtttttccg acaaaaaatc aatagggaaa taccacaatt tttcccgagt
     6121 tttattttgt gtttttggta aatacgcttg tatttatagg acggacagtt ccagatttcg
     6181 tccatttcaa cgtgatatcg ttcacacatc gtctttttcc ataaatacag cgtacacatt
     6241 ttgtgcagtc tgtatattca attgtatgaa aggtgtaaat tatccgaatc cataggttat
     6301 atattatcaa ttgggcaaaa aaagatatta gacagaaaaa acaaaccgtg gtatactgga
     6361 taaggcaaga gcgaaccaac acgctcacac gcacaacatg acatcaagcc acataggagg
     6421 acaaagacaa tgggtggaat tctggaaacg gcaatgctga tctgtttcgg ctgttcctgg
     6481 ccgatcaatc tggtgaagaa ctacaagtgc cgcagcgcaa agggcatgag cctgnnnnnn
     6541 nnnntctcct gctgatggtg ggctatatcg ccggcatcag cgccaagttt atcctgggtg
     6601 tcaatgtcca taacgcctat gtgctggtgg tctatctgct gaacctggct atggtaacgg
     6661 cgaatctgtt cgtatacttc cggaactgcg ctctggatcg caaggcagag ctttgcacag
     6721 aaccggagac agacgacacc gccatggatc tggtagctgc atccgttgcc gaacagatac
     6781 agcagcggtc gaaagagcgc acccgcacgc tccgtcccaa ggcagcagga aacgccgctg
     6841 tgctggacta tatccagtgg ggcaagacca tcgaagaata acgaaatacc tctcccctgg
     6901 agaccgccgt tatctcctac acgcatcttt gatgcggcgg tttccgatat actttccaga
     6961 aagctggtct gtttttcgac agatcagctt tttttggcgt atttgcattt tcacgctatg
     7021 gaatttgtta tatgtttttc acagaaaatt gtctatattg tacattgaca ttgtgcaatc
     7081 aaacatatat aataatggta tgtacgcagt gccgaactgc ggtaaaaacg atatgccatg
     7141 tctgcacaca aggatgcaga caaatggaca catgaggtga atgtttatga agaagaaact
     7201 gacggcatgg atcacggcgg ctgccatggg actttccatg ctgggaacgg ttgtcccaca
     7261 ggcggctctg gcggcatctg ctgccggaag tatccaggta gagcatctgg accgaggcat
     7321 ttccgccatc aacaccagtt cggggatgct ggtcagctgg cgttttctcg ccaacgattc
     7381 tgacagcgcc gtattccagc tgtaccggaa caacacgctg gtctacacca gcagcgccgg
     7441 agaatccacc tgctatctgg acaaagacgg caaaagcacg gacaaatacc gggtagacac
     7501 actggaaggc ggcaaggtgg tctcctctgc cgactgcacc atgatctcca accagaacta
     7561 ttttcagctg aatctggatc cgcccaccgg cagcggctgc acctattccc cgaatgactg
     7621 ctccgtcggc gatgcagacg gtgacgggat gtatgagatc ttcatgaagt gggatccctc
     7681 caactccaag gacaattccc agaagggaaa gaccgggaac gttttcattg actgctaccg
     7741 gctggacggc acacggctgt ggcgcatcga cctgggcaaa aacatccgtg ccggcgcaca
     7801 ctatacccag tttttcgtag cagacttcga ctgtgacgga aaggcggaga tgacctgcaa
     7861 gaccgcagac ggcactgtag acggcaaggg tactgtcatc ggcgatgcct ccaaggacta
     7921 ccggaattcc aacggctatg tgctgtccgg accggaatat tacacgctgt ttgacggcag
     7981 caccggcgca gccctggaca ccatcaacta tgagccggga cgtggcacgg tctccaaatg
     8041 gggcgacagc tacggcaacc gggtagaccg gttctggggt actgtggcat acctggacgg
     8101 cagcaagccc agcgttgtca ccggacgtgg ctattacacc cggatgaccg ccactgccta
     8161 tgatgtggtc aacaaaaagc tggtcaagcg gtgggcattc gacaccggaa acgacaagag
     8221 cgctgccggc tacggcgacg gcaaccacaa cagcatggct gccgatgtag acggcgacgg
     8281 caagcaggag atcatcaccg gctccacctg tatcgacgac aacggcaagg tgctgtggtg
     8341 cctgaacaag ggtcacggcg atgccctgca cctgggcgac tttctcccga accgtaaggg
     8401 acaggaactg tggatctgcc acgaggataa gccttacggc gtttctctgg tagatgcctc
     8461 caacggcaag attatcttcc acaaggacgg cactggcgac acaggacgct gctgtgcgga
     8521 caatgtctgg gcaggcaacg acggggcaga gttctgggga ctgaacaacg acgtctttga
     8581 cggcagcggc aacacgctca gctgccggag accggcgatc aacttcctgt cctactggga
     8641 cggcgacctg gaacgggaga tcctggacgg ctccaccgat tctcctgcca ccatctccaa
     8701 gatgggcaca gacggcaagc tgacgaccct gctctccaca gacggctatt acacctgcaa
     8761 caccaccaag ggtacgccct gtctctcggc agatatcttc ggtgactggc gggaagaact
     8821 gatcgtccgg gcagcagaca gcaagtccat ccggatctac tgcacgccct atgacacaga
     8881 ctaccgcatc acgaccctga tgcacgatcc gcagtaccgg gtacaggtct ctgcgcagca
     8941 gaccgcctat aaccagccgc cgcacgcaag cttttacctg ggttctgacc agccccttcc
     9001 ggcacgtccc aatgtcactg tactgggaca gacaacgcct acacccacac cgactcccga
     9061 tccggaacca gtaacgccta ccgcagccgt acttccggaa ggcgctgtgt tcatgctgaa
     9121 aaatgtgaac agcggtctct atctggaagt tgccaacggc agcgctgagg ctggggcaaa
     9181 tctccagcag tggggcgcag acggcgcagc ggcgcacaat acctggcgtg ccgtttctgc
     9241 cggagacggt tactattatc tgtacagcca ggtgggcgac aagatcacct acctcatgga
     9301 catctccggc ggcaaggcag acaatggcac aaatgccgca ctctggacaa agagcagccg
     9361 ggaacaggca cagcagttcc tgttcgctaa aaacagtgac ggttcttaca ccatctatac
     9421 cagggcatct gagctgaaaa gctgtgtgga agtcgcagac gccagcacgg caagcggcgc
     9481 aaatgtacag cagtatgcgt acaacggcaa tgcctgccag cactggaacc tggaagaagt
     9541 ggctgacacc ggcgtaaagc tggacaccac aaaggtatac cagttccaga acagcaacag
     9601 cggtctgatg ctggaggttg ccaacgccag cactgaggct ggggcaaacg tacagcagta
     9661 cgccgaaaac ggcaacaact gccagaagtg gacgctcaag gaatttggtg acggctatta
     9721 ctacatcctg tcccagctga gcgacggaaa gacctattac ctggatgttg ccgacggcaa
     9781 gagcgatgac ggcaccaaca tccagatctt caccaacaac aagtcctcta cccagctgtt
     9841 caagttcatc cccaacccgg acggcacgta ttacatcgtc acacgggcga ccagagacaa
     9901 aggcggactt ggcgtttccg gcgcatccac ggacaacggc gcaaacgtca tccagtggac
     9961 gaaaaacggc aacgccgacc aaaagtggat cgtcaccgcc atcggcgaca tcaccgctcc
    10021 ggtcactacc accgaaccgc cggcaacaac actgcccaca gagactacca ccagcgcagc
    10081 acctaccgaa acgactgctg caccgacaga aactactaca tccgcagcac ctaccgaaac
    10141 gactgctgca ccgacagaaa ccactacctc cgctgcaccc accgagacaa ctgccgcacc
    10201 cacggaaacg actacttccg cagcacctgt gaccgtcaca gagactgttc cgaccgaaac
    10261 cacctctgag acaccggcgg aaacatcact cgccacaacg gttacaacaa ccaccactgc
    10321 cgctccggca tccagcgaca cgaccacctc gaccacaaag gcggcgccgg tttcctcgat
    10381 cctctacgga gacaccagca tggacggcag agtggatatc acagacgcag tcctgctgaa
    10441 taaggcgatc gccggcgcag tagaactgga cacaaacgca cgccagaacg gcgactgcaa
    10501 acaggacggc gaactgacac tggaagatgc agtgacactg ctgcagttcc tggttcacgt
    10561 catcagcagc ctgccgcagt aaaaaagcct attccgtacc taacccaatc ccaaaacagc
    10621 gccgtttccg gaaaatccgc cgggaacggc gtttttctgc gataccgttt gcaagatttc
    10681 ccctttctct gcatagaata ggattgtatc cctatggcaa cacagaacgg aggttatttt
    10741 atgtgcggaa tcaccggcat gatacatctg gaacaagacc tgcggcagca agaggcgctg
    10801 ctgcgcaaaa tgcaggagac cctgcgccgc cgtggcccgg atcaggaggg catttttctc
    10861 tcgccacgct gtgccatggc gcacacacgc ctggcggtca ttgacgtgga aaagggcaga
    10921 cagcccatgg agatcaccgc cggcggcgag acctatacca tcgtctacaa cggcgagctt
    10981 tacaacacgc cggaactgcg cacacagctg gaacaggagg gcgtggtttt tcagacccac
    11041 tgcgacaccg aggtcgttct ccagtcctac gcccactggg ggccacgctg tgtggaacag
    11101 tgcaacggca ttttcgcctt tgccgtctgg gaacacaccg cccagcggct ctttctggca
    11161 cgtgaccgca tcggcgtaaa gcctctgttt tatgcggaga ttcccggcgg tctggtgttc
    11221 ggctcggaga tcaaggcact gcttcagcac ccggatgttc cccacgagat cgaccagaac
    11281 ggcgtggcag acctgctgct tttgggaccg ggacgcacac cgggctacgg cgtgttccgg
    11341 acgatccggg aagtcaagcc ggcgcactgc ggctactata tgcagggcag tctccacctg
    11401 catccctact gggaactata tgcggcagtc cacaccgaca gctttgccga aactgccgcc
    11461 cacgtccggg aactgctcac cgacgctgtc cggcggcagc tggtgtctga cgttcccatc
    11521 ggcacatttt tatccggcgg tctggattcc agcctgctct cttccctgtc cagccaagtt
    11581 ctccgggaac gtggagaaat gctccacacc ttttccgtgg actaccggga caacgaccgc
    11641 tatttccgaa agagccattt ccaacccacc agcgaccctg cctatatccg tgccatggag
    11701 cgctatctgg gctgtcagca tcactggacg gtgctggata cgccggcgct ggcagaggcg
    11761 ctttatgccg ccgtggatgc cagagacttg ccgggcatgg tggacgtgga cagttccctg
    11821 ctgctgttct gcggcgagat ccgaaaggaa gtcaccgtgg cgctttccgg cgaatgtgct
    11881 gacgaactgt tcggcggcta tccctggtac cgggatgcag atgtgcgccg ggtcaacggt
    11941 ttcccctggg cgcagagtac cgcctatcgg gcaaagttcc tccgtccgga gctggcgaaa
    12001 aagatcgacg cccaggcgta tgtccaggat gcctatgaac gcaccatccg tgccgcagaa
    12061 aagctgccgg aggattcgcc gctggaatcc cggatgcggg aaatgatgaa actgaatctg
    12121 gactggttca tgcagactct gctggatcgg aaggaccgga tgagtatgta caattccctg
    12181 gaggtgcgtg tgccgttctg cgactaccgc attgccgagt atctgtacaa tgtgccgtgg
    12241 gaatacaagg actggaacgg ctatgaaaag ggactgctgc ggacggcgat gcaggatgtg
    12301 ctgccgccgg aggtgctgtg gcgcaagaaa agcccctatc ccaaaaccca caaccccagc
    12361 tatctccggg cagtgtccga aatgctgcaa aaggtgctgg acgctccgga tgctccggta
    12421 ctgcagatcg tccgccggga aatgctggaa cagctattgg acagcgaaga gtcggtgcag
    12481 tggtacggtc agcttatgac ccgtccgcag atcatggcgt acttcgtgca gatggactac
    12541 tggatgcgga aataccaggt gaaggtcgtt ttctaaggca ataccgcaca aaccacaaag
    12601 agccgcaggc ttgcttttta cagcagtgcc tgcggctctt ttgttatggt tgatttattc
    12661 tgcttgttcc ggattctgcg tcagcatggg acggttgagg atctgcatga actcttcccc
    12721 ggtgatggtt tcgtgttcgt acagatactt ggcaatctca tgcagcttcg gcatatgctc
    12781 ctggagcatt tcaattgcac ggcgatactg ttcgccgatg atctctgtga cctttttgtc
    12841 gatcttggtc tgggtctcca tggaacaggt caggctggcg tcgccgccca gatactggct
    12901 ctgcaccgtc tccattgcca ccatgccgaa ttcatcgctc ataccgtagc ggctcaccat
    12961 agagcgtgcc agcttggttg cctgctcaat gtcgttggat gcgccggtgg tgatctcgtg
    13021 gaagatcagt tcctctgcgg cgcggccgcc ggtcagcgtg gcgatcttgt ttaccatttc
    13081 tgtcttggtc ataaggtaat gctcgccctc atccacctgc atggtgtatc ccagcgcacc
    13141 ggaggtacgg ggaatgatgg tgattttctg caccggcgca gactgggact ggagcgcagc
    13201 caccagtgca tgacctacct cgtgatacga cacgatgagc ttttccttgt tggaaagcac
    13261 ccggttcttc ttctgatagc cggcgatgac cacttctacg ctctccatca gatcttcctg
    13321 tgtcgcacag gagcgtccca gacggacagc acgcagtgca gcctcgttga tgatgttcgc
    13381 cagttccgca ccggatgcgc cggcagcagt ccgggcaatg gcggaaaagt ccacgtttcc
    13441 ggcaagcttg atcttctggg cgtgtacctt cagaatgtcg atacgtccct ggaggtcggg
    13501 cagttctacc ggaatgcgcc ggtcgaaacg tcccggacgg agcagcgccg ggtcaaggga
    13561 ttccggacgg ttggtggctg ccagaatgac aacgcccttc gaaccgtcaa agccgtccat
    13621 ctcggtcagc agctggttca gcgtctgttc ccgctcgtcg ttgccgccgc cgtactgtcc
    13681 gccgtcacgc tttttaccga tggtgtcgat ctcgtcgata aagacgatac acggggcttt
    13741 ttcatttgcc tgcttgaaca ggtcacgcac ctttgcagcg cccatgccca cgaacatctc
    13801 cacaaattcc gaaccggaaa tggagaagaa cggcacgttt gcctcgccgg caactgcctt
    13861 tgccagcagc gttttacctg ttccgggagg tcctaccagc agtgcgccct tgggcataga
    13921 tgcgccgatc tctttgtagc gctccggctt gtggagataa tccacgatct ccgccagcag
    13981 ttcctttgcc tcttcctcgc cggcaacgtc cttgaacttg atgccggtgg tggacttcac
    14041 atagaccttg gcgttgctct tgcccatgcc gaacatcatc gagccgtcgc cgccgcccat
    14101 acgctgcgcc atcttcttgt taaagaagtg gaacaccagc agcatcagcg caatgggcaa
    14161 aacatagctg attaaaatgt acagcagcgg actggtgccg ctgtcgatct gggactcaaa
    14221 ctttgcgccg gagttataca gacgctggat cagatccggg tcgtccagcc gtccggtctg
    14281 atacacctgc ttttcgctct tatccgtaaa ggtgatctgg ttgctttcga tggtaactga
    14341 accgatctca ttgttctctg tcatgctgat aaaggtctca tagccgactt ccttgacccg
    14401 acccttgaac aggttcggga acacgaacgc ctgcagcagc agaaagacgc acagggcaat
    14461 gatccagtac gagatccacg atttttttgg tttttgcggt tcttccatta atacattctc
    14521 ctctctctgt accgcagtaa aacacatttt cctgcggcgg ggatttttca aaagcggaac
    14581 ggtgattccg ttgttctata tcttactata gctttttgtg aaacaaaagc gcactttgtg
    14641 atagttttat gagaattgcc gaaaggtctc taaaagatat tttctgcaaa taccagcacc
    14701 tgatagtttg tgccgtagct gtccgtcagc acaaacgaac gctcagcccg gctcagcagc
    14761 ggatctttcg ctgccgcaag cacctggtat tcctcggtat ccagcagcag gaagtagtcc
    14821 tcctgtcccg gctggtcatc ataccaggca gctgcggtct gatagacggc tttctgcacg
    14881 ccgtcctcat ctacattgac gttgcgcacc ttcagcttgt cgccggagat caccgtcatg
    14941 gcgttggcgt gccagaatgt ggcataccca taggtcagcc cctgctcttc cagcatattg
    15001 gtcagctggt actgcacgtt gacctccgcc gcattggtgg gcagcagcag gatgttccag
    15061 gcactcagca ggcaaaagca cgccaccggc acacagagca gtcctgccac acgctgcatg
    15121 gcagtccgct tggtaattgc ccagcggaga aataccagag acacgatgga cgctgtgcag
    15181 acaatggggc tgagccgcca gttcgccacg gaaagtgcgc cgcagagata gcccaccagg
    15241 atcagcccgg tgactgtcca gtgcgcccag agcagaatgc gcatctgtct gccggactgc
    15301 cccttatact tggggtagca gcaggtcgcc gcaaccggca gcaccgccaa aatcagcgcc
    15361 gtgccgatgc ggatcagatt catgacgctc tcaccggaca tcagcttttc gccgggaata
    15421 tcttccagcc ccagcagcgt cagccacgcc agcggcagtt tctgcaaatg ctctgtccag
    15481 gtgctttggg cagtccagtt ggaataggca tccgcataag ccccggtgac gccgtttgcc
    15541 cagatgttcc ccagttccat gccggagaca actgcgattc ccagagagag cagcaacagc
    15601 agcgcacggg tgttcttcca gtggagcagc ggcgttttcc gatccagcag ccgttccagg
    15661 aacagccctg ccaggatagg cagcgcaaag atcgtcaccg cagtgacctg gtcggtgcag
    15721 caaagcacga aaaacaggat cagcgcaata aatgtcagaa tggaatgcac ccgtattttc
    15781 cgggtctcct gatgcactgc caccagattt tgcagccgga acaacagcgc cagtccgcag
    15841 aacaggaaca ggatgcccaa gctataatag atggtatgtc cccagaagat ctcccggagc
    15901 tttttgctgg cgctgagaat catgaggaag atcgccgctg tggtacagat ccagcgatag
    15961 tcccagtgca tttcccggag catccagcaa agcaccgccg tgagcagcag gaaaaagagc
    16021 agcatcccca gcatatgggt cgtcatggac agcccgaaaa agggcagaaa cagcagcatc
    16081 agcaggctgc cgccaaaggg cagcagacag gcgtaggaaa agtccgggct gataagcttt
    16141 ccgctgtcga aggttgcctt tgcccagtag atcgtatccg tacagtcggc gtggaactcg
    16201 ccctttgccg gacagatgat gtaatacgcc accagtccca gcgccaccac cagcagcacc
    16261 ggcggaaata ccatgcaaaa gctctcccag aacagctttc ccagctgttt tctgggaacg
    16321 ctctccggca ccggctcggc ggaaatggca gccggttcca ggtcagacgg tttcagcccg
    16381 tccggttcgg tcggctcgtc cggcggcaga actgcatccg gattggaaac ggctatgggt
    16441 gtatccggtg tatccgtatt ctccgccggg cttgtctccg gaacagcgtc ctgctccggc
    16501 acagtctccc acggatgttc ttgttcggtc atagatcgct ccttttctca gacagaggca
    16561 gttccggtga gtacttctgc cggatagttc agctgttccg gtgtccacgc cgccataatg
    16621 ggctgatact cccgtgccat tgcctttgcg ccctccagat catgggcaag gtagttgccg
    16681 cactcttggc gcttgccgcc gggaatctct ccggtgaagt ccagcacata ttgaagtgtc
    16741 tcctgcacca ggcgaatggc atctgcgccg gaaacggtat cccgtgtgag gaaatagaac
    16801 ccggtgcggc agcccatggg tcccacatag accacaccgt ccgacagcgg cgtattgcgc
    16861 acataggtgg caaagaggtg ttcgaaagtg tgcagggcgt tgtcctccag atagtcgcct
    16921 gccggattgt tgggcagttt catgcgcata tcataggtca caacatctcc gtccacacgg
    16981 gaaatgtaca taccgggttt cagataatca tgattgatgg taaagcttgt gatctgtttc
    17041 attgcagtaa cctccagggt cgtgattccg cacagcaccg tgcggttttt ggtttctttc
    17101 attatagcaa aatcggggcg atattgtcaa ggaatctttg cgaatcgaat gcaaaaaacc
    17161 gggctgcgtg ggcagtccgg ttttgcaggg ttgcttaaaa ctagatgctt attccgtact
    17221 cggcagtgat gtgataatat gcaccaaaaa ctgcatcagt acaatcgcat catttgcacc
    17281 gatctcatca tctccattgc agtctgcatt tttctgtgcc gtactgtcca gtgaaacagc
    17341 accggcacaa gccttgttca gcagaaccgc atctgcgata tcgactctgc cgtcctggtt
    17401 gacatcaccg atcagatatt ttgcctgttc cagatgatct acagtaaatg ttactgtaat
    17461 attttgcatg actgcagtat tctttgaaag atcgttgacc tttccaacct ttgaaacacc
    17521 ccaggtatct gtcagataaa tcctggtaaa ccctttgtca tccgcataat atgcagtatc
    17581 aactgcacca tctgatgcag aataatccac cttttttccg tctacaaaaa cagaatcaat
    17641 agttaccgat aaatcggggt atgtatcaga ggtaatgaaa ttatcatctg ccgaatccaa
    17701 ttccagcact agaaattcaa tcgttcctgt accgtctcct gtaatatccc aggatgcaac
    17761 gtactgtccg gagcctgtca gctgtgcagc atgactggtg acatcatccg tgttacgttc
    17821 ttcatcaacg ccccagtact gatagccgcc catctgtcct atcaatgtag ccgttcccaa
    17881 aacgccggtc ggtttactat cgggaacttc caatgtaaca gaactcatag cgccatcata
    17941 agagactaca atggatgttg tgcctgcgcc cacgatcgtc acacaaccgg aattatccac
    18001 tgttgcaaca gatgtatcgg tagagtacca gattcctgcc ccgtcaggta cattgctcac
    18061 caacaagaaa ctgtttccca cacgcagcaa attctgtgcc ggcggctcaa tggtgagcgt
    18121 ttttgcggtg gttgtggtgg ttgaagcagt tgttaaagaa gtctgtgtta ggctggttga
    18181 tgaaccatcg tcttcgacag taaagcttgt tgaacttcct acaacccaaa ctttatatcc
    18241 atatnnnnnn nnnncatatc caggaccatc ataatgataa tcccaaattc caatcgtaac
    18301 gctgccagtt ccaacaattg taactgttcc tgtattatct acagtagcaa cagactcatt
    18361 actcgagtac cattgacaaa gctcactagg agcctggact gaaaatctat actggtctcc
    18421 aatatgtaaa tctaaaccag gtgggagtaa tctcgttcta tataccatac aaacttctgt
    18481 cgttgtagtt gtagatgtag ctaaaggatc tatgactgga attgtttcag aatctgttgt
    18541 tgtagttttt acatcagtcg ttgttgtggc ggcagtagta gtagtggtag ttgttgtggt
    18601 tgtggtggtg gtagttgttg ttgttgtggt agttgttatt ttgggcaaaa cataatctcc
    18661 atcactgcat ttcactgttg ttccacctaa ataatctgga caaaaagcac tccattcagc
    18721 acatgttcca ttgtagaaaa tgcaatttga catatcatag tagcttccat tttttacaca
    18781 atagaatgag ccgtctgtgc aaagcaccga tgtaccaggg taaaccttac acatagaaag
    18841 atacttttca caagtcccat tatactgaac aattacttca ctgtgaatat ctccaatttt
    18901 agtaattgta ctgggaataa tcaaaatagg cggtccccaa tggctagtgc ctccactacg
    18961 attcaaatgg gatacttgga tgtccccaat ttcagtaact ggaatattat ctatcatctc
    19021 cggaatttca acatttctgg aaaagcgccc ttcataaaaa gattcgccaa caaccgaaat
    19081 atcactatca taataatatt catctctgtc agttgtagtt gtatcataac cagtggtaac
    19141 cagtatacat gatgtagaaa cagatactcc tgtgatctta atatgtttat caagagcagc
    19201 atcgtctcca ggagcatctt catatgattt tgttagaatg acatagctga ttgtaaaaga
    19261 ccagtccatt ttccatccac aaaaatcact tttataccct cctgtataat actttgtttc
    19321 cgaagcagtt tccccgcttg ccgcaaaaac gtgttcctgc agcatcaaag cagattccgg
    19381 cagcgcccct gtaccgacaa agcacagcac gccggcaact gctgctgcgg caatttttcg
    19441 taaaaagccc atacacaaat ccctctttct gtttttttca ctgccagata ccgacagcat
    19501 ctattcgtaa ttataccacg aaatatgcaa aatgtcaacc catacgcaaa aaagccgtca
    19561 catataactt cggtatgcga cagcttttgt ttttatttat gcctcggtcg gtgcagagga
    19621 ttccccgctc tcttccggct tttcctcagc cggtgcagca tctccgttct ccgccggttc
    19681 tgtttcggca gtttcttctg gttctgccgg aacctcttcg aactgtgccg gatccatctt
    19741 accctgcatc agtgtctcga agtcgtcctt ttcgatcttc tcgtggatca tcagatactt
    19801 cgccagcagt tccagctgat ccatgtgcat ttgcaggatc gccgttgcct tgtcgtatgc
    19861 cttttcaatg atctcacgta cttcatcgtc gatctctccg gcaacacggt cagaataatc
    19921 ccgggacgaa cccaggtctc tgcccaggaa aacctcgctc tggtcgttgc cgtagaccac
    19981 cggccccagc ttttcgctga aaccataacg tgtcaccatg ctccgtgccg tagaagtagc
    20041 acgttccagg tcgttggatg cgccggtgga aatatcgtcc agcaccagct gttctgccac
    20101 acgtccgccc agcagggtga tgatgttctc ttccatttcc ttcttggaaa caaagctctt
    20161 atcctgctcc ggcagggaca tggtatagcc gcctgccata cctctgggga taatggacac
    20221 ctggtgtacc ttatcctggt ggtcgcagta ataggtgcag actgcgtgac ctgcctcgtg
    20281 gaaagcggta aggcgctttt ccttgggcgt caccttatgg gacttctttt ccggtccggc
    20341 aatgaccttg atggtcgcct cctggatctc ctccatggtg atggctttct tgtctttccg
    20401 ggctgccagc aggcacgcct cattcaccag gttttccaga tcagcaccgg taaagcctac
    20461 cgtcgatttc gcaatggtgg aaagatcgac gtccggaccc atgctcttgg tcttggtgtg
    20521 tactttcaga atctgctctc tgcccttgac atccggatag cccaccacga tctggcggtc
    20581 gaatctgccc ggacgcagca acgccgggtc gaggatatca cggcggttgg tggcggcgat
    20641 aacgatnnnn nnnnnnataa cgatgacgtt atcctttccg gtaaagccgt ccatttccac
    20701 cagcagctgg ttcagcgtct gctcccgttc gtcgtgtccg ccgccgatgc ctgtgccacg
    20761 gtgacggcct acggcgtcga tctcgtcaat gaagatgata gacggtgcat ttttctttgc
    20821 ctggtcgaac agatcacgga cacgggatgc acccacgccc acgaacattt ctacaaagtc
    20881 cgaaccggag atagagaaga acgggcagcc tgcctcgccg gcaacggctc tggcaagcag
    20941 tgttttacca gtacccggag gccccatcag caggacaccc ttcgggatgc gtgcgcccag
    21001 ttcagcgtac ttccggggat tcttcaagaa gtccacgatc tcttccagct cgtgcttttc
    21061 ctcatccgca ccggcaacat cggcgaaagt cgcctttcgg gtgttcgcct ggttcttgat
    21121 gttcgccttg ccgaaagaag tatacttgcc gccgccgttt gcctgccgca tcatgaacac
    21181 aaacaggagg atcatcagca ccagcagtac cagcgtaggg atcagcgtca gcagccagga
    21241 ggtgtcacgg atcttgtaat agtccacctg cagcggctga tccggatgct tttcattgta
    21301 gtccttgcga taggtctccg tatcctggcg gaacacggaa acattgggga catcataggt
    21361 gagcttgtcg ccgttttcca gggtcagttt cagttcgccg gaacccagat ccagcgtgta
    21421 tccggtgacc tcatagttgt cgaagtactt catgatatcg gaatacttcg tctccttgcc
    21481 gccgcctttc atgccggtca gcaaaaagac cagcgcaccg atcagcacaa caaaaacgag
    21541 cagatacgct ccgatcccac ggcctttctt tggtgtcaaa tcaaattcac tccattctgt
    21601 tgaaaaagag acgcagcccg gcggtctcac cgttcctgtc tctatgttgt ttcaaaccat
    21661 tccagcacca gcagccggcg tgtcttttcg tccgggcaga ctctcgccgc acagcccaca
    21721 ccccggacgc agaccacgcc ttcgccgtct gccagaatat acgctgtact gcggcgctcc
    21781 ggcggcagat tcgcctggat cagctttttc accgcagagg taaaccctct gcccgggagc
    21841 tgcatccggt cgcccggctg ccgggggcgc aggaacattt caccttttat tttatcataa
    21901 tccagcaact catttgcaga ttttttatga acattttcgt ttttttccaa ttcttctggg
    21961 gatatcagtt gtgccgcaag cacccagccg gagtccagcc ggttctctcc cagctgcacc
    22021 cgtaccggtt ccaggatgcc ccgacgtttc ggacaggtct ctatggtcag tctgcccgat
    22081 tttcctacgg cgtaaacatc cccggacaaa ttccacctgc cgccctgccg ggtcagccgt
    22141 tccagccgtt ccagcaaagg cgcatcgcag gaaaagccgc attcctccag gagcatggca
    22201 atacagcgaa accgcagcgc cggatgcagc tgttccagcc catccagggt gttttctgcg
    22261 gtgtgacaac agcggtacgc cgccgatgcc tgctgtgtca gaaaattgtc ctcccgacgc
    22321 agactccgca gggtatagga aaccgtccgc tctgccgccg ggttcagcgc ctgcattgcc
    22381 ggaatgatat ggtggcggat cctgttccgg gtgtagtcat cggtcagatt ggtgctgtct
    22441 gtcacataag tctgttccag cgccgccagg tgtgtctcca cctgttcccg tgtgatgcgc
    22501 agcagcggac ggagaaatct gccgtttctg ggtggaatcg ccgtcagtcc gtggagactc
    22561 gccccacgca ccatccgatg caccagggtt tccaggttat ccgacgcagt atgcgccgtg
    22621 gcaatccagt ctgcccgcac ctgtgcaaac gcctcatagc ggcacatccg ggcagccagt
    22681 tcctcagagc attgctccgc cgctgccctc gccgccgcat ttacgtccag tactgtcaga
    22741 tacacaccca gcgctgcaca gagccgccgg caaaactgcg catcccggtc gctctctgcg
    22801 ccacggaggt ggtggttcac atgaaccgcc gtgaccgtga ttcccagacg ctgccgcagt
    22861 tccagcagac actgcagcag gcagacactg tctgcgccac cggaaagcgc acagcacact
    22921 gtctgccccg gcagtacaca gtccagttcc cggagctgtt ccagcaccgc acggggcagt
    22981 gcatcagccc tcattgcgca tttccgcatt ggagaaccgt gtgaactgac catcccagac
    23041 cagctggatg gtgccggtct cgccgtgacg gttcttggcg acgatacact ctgaaatgtt
    23101 cggtgtctgg ctttccttat tataataagc gtcccggtag aggaacatga caatatctgc
    23161 atcctgctcg atagaaccgg attcacgcag gtcggacagc atggggcgct tatcggtacg
    23221 gctctccggt ccacgggaca gctgggacag cacgatcacc ggaatatcca gctctttcgc
    23281 catgattttc agctgacggg tgatctcgga gatgataagc acacgattat ccgtccg
//
LOCUS       DAWENO010000012        23333 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_906_length_23333_cov_12.543217, whole genome shotgun sequence.
ACCESSION   DAWENO010000012 DAWENO010000000
VERSION     DAWENO010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23333)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23333)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23333
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_23333_cov_12.543217"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4747..4756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12601..12610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19498..19507
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4797 a         6957 c         6224 g         5325 t
ORIGIN      
        1 cacgcatcat gtcccccttg aaaaggcgcc cgtagggtgt gacttgtgct gggatgtccc
       61 gtagggacag ggggattcaa ctctcaaaat ggttcaaaat gtgcatctgt aggggcgaac
      121 actgttcgcc cgttggtgcg tcactcctcg tttatccctc cgtctgctcc ttacggagca
      181 tccacctccc ttttcaaggg aggcttatta ccacgcatca tgtccccctt gaaaaggcgc
      241 ccgtagggtg tgccttgtgc tgggatgtcc cgtagggaca gggggattta actctcaaaa
      301 cggttcaaaa tgtgcatctg taggggcgaa cagtgttcgc ccgttggtgc gtattactcc
      361 tcagtcagcc ttacggctgc cagctcctct acaagaggag ccttatatcc cccatctgcc
      421 tcctctttag aggaggtgtc cgaaggacgg aggagtcacc acccgcttac ttaaagtaag
      481 ccacgccgct ctcgaagatc ttctgatcct tttcacccgg cacgttccgg tacaggttct
      541 gaccgatacg ctcgctgtga cccatcttgc ccagaactct gccgtccggc gaggtgatgc
      601 cctcaattgc catcatggaa gtgttggggt tgaagtggat gtccatggtc gggttgccgt
      661 ccatgtccac atactgggtt gcgatctgac cgttcttcgc catgtctgcc agcagctccg
      721 gcggcgcaac aaagcgtccc tcaccatggg agatggctac ggtatgcaca tcgtccacct
      781 cggtgccgta cagccacggg gacttgttgg atgcgatgcg tgtccgaacc agcatggact
      841 ggtggcgtgc gatggtgttg aaggtcagcg tcggcgaaac gtcggtcatg tccatgatct
      901 cgccgaacgg taccagaccc agctttacca gtgcctggaa gccgttgcag atgcccagca
      961 tcagaccgtc acggttcttc agcaggtcgt gtacagcgtc cttgatgcgg ggattgcgga
     1021 agaatgcggt gatgaacttg ccgctgccct ccggctcgtc gccgccgctg aatccgccgg
     1081 ggatcatgat gatctgcgcc tggcgcacca tagcggcaaa ggtggtgatg gaatcttcca
     1141 tcgccgcagc ggaaaggttc cggacgataa aggtctgggg tcttgcgccg gcacgctcaa
     1201 agacacgtgc ggtgtcgtat tcgcagtttg tgccggggaa taccgggatc agaacacggg
     1261 gctgtgcgct cttgatcgat gcggacaggg tggtctttct cggataggac agcttttccg
     1321 gaacttccgt ggtggtcttg atacggcacg ggaataccgg ttccagtttc ttttcccaga
     1381 cagccagcag cttgtccagc tggagagtga acttcgggca gacgatctcc ggcttttcgc
     1441 tggtagaacc gatcacatcc tcgccgtcct ctgcatcgcc gcagagttcc agcaggaatc
     1501 cgccgcagca gggctggaac agctctgcgt ctgtcagctg gcggtcgaag gtaaagccga
     1561 tgccgttgcc gaagcacatc tttgcgatac cctcagccac gccgccgtag tcgatgaccc
     1621 agccggcacg gatgcggcct gcggcgatca ttgcctcgac cttgtcgaat gtggcacgga
     1681 tgctctcaaa gctgggaacg ccgttctcgt cggtctccgg acggagcata acgaccttgt
     1741 cccctgcctg cttgaactcg gtggagacca ccttgtctgc cttggctgtg gaaactgcaa
     1801 aggataccag cgtcggcggc acgtcgatgt tctcgaaggt gccggacatg gagtccttgc
     1861 cgccgataga gccgcaggac agttccagct gtgccttgaa tgcgcccagc aatgctgcca
     1921 taggcttgcc ccagcgcttg gggtcgttct gggtgcgctc gaagtattcc tggaatgtca
     1981 gccagcagtg gtgccagctg ccgcctgctg cgactacctt ggcgatagaa gtgataacgg
     2041 catccattgc gcccaggtac gggttgcgct cgctgacggt gggattatag ccccacgcca
     2101 tgagggagca ggtgttggtc tcgcccttga gtactgggat cttggcagcc atcgcctggg
     2161 acggagacag ctggtatacg ccgccgaacg gcatcagaac ggtgcctgcg ccgatggtgg
     2221 aatcgaactt ttcgatcaaa cccttctgcg agcagatgtt cagactggac agcagctctg
     2281 tccacgcatc tgcgctgtcg gtgagggtgg attccgcagc cggctggggt tcttcgatgg
     2341 cgatgccggt gtgctttgcc gcaccgttgg agttgaggaa ctcacgggac aggtctacga
     2401 tggtgttgcc gttccagtgc atccgcagtc tcggctcagc ctggacagtt gccacgatgg
     2461 tagcctccag gttctccttg cctgcctcct tgatgaactt ttctgcatcc tcggcagcga
     2521 caaccacagc catacgctcc tgggattcgc tgatggcaat ttccgtgccg tccagaccgt
     2581 cgtacttctt cggtacagcg ttcagatcaa tttccagacc gtcggtcagc tcaccgatgg
     2641 caacggaaac gccgcctgcg ccgaagtcgt tgcagcgctt gatcatctgt gttacggtcg
     2701 ggttgcggaa cagacgctgg agcttgcgct cttccggtgc attacccttc tgtacctctg
     2761 cgccgcagtg acccagggac tctacggtgt gggacttgga agaaccggtc gcaccgccgc
     2821 agccgtcacg tccggtcttg ccgcccagca ggatgacgat gtcacccggc accggtgctt
     2881 cccggcggat gttggatgcc ggagcagcgc ccagcacagc gccgatctcc atgcgctttg
     2941 ccatatagcc cgggtggtag acctcagcca cgtgaccggt cgccaaaccg atctggttgc
     3001 cgtaagagct gtagccgttt gctgcgccga cagtgatctt tctctggggc agcttgccgg
     3061 cgatggtatc ctctaccggt accagcggat ttgccgcacc ggtgacacgc attgcctggt
     3121 atacgtagga acgtccggac agcgggtcac ggatcgcacc gccgagacag gtcgccgcac
     3181 cgccgaaagg ttcgatctcg gtggggtggt tgtgggtctc gttcttgaac atgaggatcc
     3241 agtcctcttc cttgccatca atgtccacct tgatcttgac ggagcaggcg ttgatctcct
     3301 cgctctcgtc caggtcgttc agcttgccgt ctgccttcag tttcttggca gcgatggttg
     3361 ccaagtccat aagggtcatg ggcttttcgc ctctgcccag ttcctggcgg acggtgcagt
     3421 atgcgtcgta ggtctccttg atgtaatcgg tggggatatc cacgtccgtg atctgtgtca
     3481 ggaatgtggt gtggcggcag tggtcagacc agtaggtgtc gatcatgcgc acttcggtga
     3541 tggtggggtc acggtgttcg gtgtcccgga aatactgctg gcagaactgg atatcgccca
     3601 gatccattgc cagaccgtag tcggcgatga actttttcag ctcgtcctcc gacagaccga
     3661 taaagccggt gagggtctct acggtagtcg ggatggtata gttggtatcc agggtctccg
     3721 gcagttccgg agatgcctcc cggcgctcta ccgggttgat aaggtatttc ttcagacggt
     3781 cgaactcagc gtccgtgaga ctgccggtga caacatagac gtctgcggtg cggacacggc
     3841 agcgctcgcc ctggagcaga atctggatac actgttcaca gctgtcggca cgctggtcga
     3901 actgacccgg cagatactct gtggcgaaca tccgctgacc ctcttccagt gccggcagtt
     3961 ctgtgtaggt gtggtctaca gcaggctcgg aaaatacggt ggggacagca cgctcgaaag
     4021 cctctgccgt cagatggtct gcgtcatagc ggtggaaaat gcggatgtcg ctgacattca
     4081 gacggagcgc cgtctgcaca tcgctcagaa ccgagcgtgc ctcaacggcg aattccggct
     4141 ttttctccac ataaatacga aaaacggaca tggtaaatct cctttatttt gaatttgcgg
     4201 atgctgctgc ggactggctc ccctataggg cacccgaagg gcgtgccttg tgctgagctg
     4261 gcaagccgca ggcttgactg aggggtattt ccgtgaaaac tagcacatga agaaaaaaga
     4321 ttccgcaaaa cccctccacc agcttcgctg gtccccctcc cctttcaggg gaggctgatt
     4381 cggcagactt gtacaagggc agcagctgtt tgctgttttt tctgcacacg ggtcgatgtg
     4441 ggcatcgacc cctacaggtc ttcgtcaaga ccggactctg tcagcgtccc atagcagcat
     4501 tccgcatcac tctcttctat tctaacacaa aaaatgcgat tacgcaaact tttttttgga
     4561 ttttttgcag aaattcgcac aacggtgccg ttcggggtgt gtcccacgtg atagccgtcg
     4621 ccacgctcac gttcaaaaag caccggaaat tccgtgccga tctggctttt cagaaagttt
     4681 tccttggcgg tcttcgccgc tttctgcgcc tgttccatcc gggcagtttt ttctgcgccg
     4741 ctgcacnnnn nnnnnncgcc gcagcccgtg tgccggctct gggcgaatag cggaacacgt
     4801 gcatggcggc aaacgccatc cgctgtgcaa acgccagcga gtcccggaag tcctcgtccg
     4861 tctcgccggg aaagcccacc atgaaatctg tggtaatggc gcagtcggga aagtaccgcc
     4921 ggatgcgctg acagatgacg gcatactcgg cgctgtcata tttccggttc atggcacgca
     4981 gagtcctgtc acagccgctt tggagagaca ggtggaactg gggacagaac tggggcagcg
     5041 ccgccagccg ggaaagctgt tcttccgtca gccgctccgg ttcgagagac cccagccgga
     5101 cacgctccac gccggggacg gcggcacagg tctccaccgc ctctgccagc gagccgtccc
     5161 actccatgcc gtaaaagccc aggttgatgc cgcagaggac gatctcccga tagccgttct
     5221 ccgcaaatcc ggcggcggct ttccgcagtt cctccggcgg caggctccgg cagcgtccac
     5281 gggcataggg aatgatgcag tagctgcaaa aacagttgca cccgtcctgt atcttcagaa
     5341 aggcacgggt gttgtccggc atggtgtcgc agggcagcgc ctcgaagtca tcctgtgcgc
     5401 cgtagctgtc cacggcggcg atccgggcat ggttctgaca cagctccgcc agcagtcccg
     5461 gcagcctgct ccggtgtttt gtgccaagca ccaggtctgc ctccggaatg gcggctgcct
     5521 cgtcgggata cgcctgggga taacatcccg tcagcaccag cagagcatcc gggtgttccc
     5581 ggcgcacctt tcgcatggcg tggcgcaggc ggctgtcccc ggatgcggtg acggtgcagg
     5641 agttgaacag gaaaatgtcc gcctcttccg gctgagagac caccgtatag ccggcgcttt
     5701 gcagcagaga ctgcatcccg gcggtctcac aggtgttcac cttgcatccg aatgtataaa
     5761 aataacagtt caagtctgtg cgatacctcc tcatctccag aaaaacgacg gaatttccga
     5821 aaaaacgacc gggtttccgc agaatctttg cgccgttttc actggatttt catgaaaatt
     5881 aacgtcactg tcaaaaaaac aggggtgttt ccatcttcta ttatactata tcgacgaaat
     5941 tgttgcaatc gtcttgacaa gaaaattttt tcgtggtaga ctataggtac agaacgggaa
     6001 agcacccgga gcttgcacaa tgaaagcgaa aggcggtatg cacgttgccg ttttatggga
     6061 tgtgcttttg gttctgcaat ttattgtgac accggaaggg cgagaatcag accccttcaa
     6121 gaggaggtaa ttgttatgta tgaaacaaca attcagctga atgcgatcaa cgatgtaaag
     6181 gatttcgtaa acaaggttat gctgttcaat tatgacattg atctggtgtc cggccgttat
     6241 gccattgatg caaaatccat catgggtatc tttagcctgg atctgtccaa gccgatcaag
     6301 ctgcaggcac acacagacga tgcagacaag ctggtagagg ctatcggcaa gtacatcgta
     6361 aagtaagttt ttagaacaca catctttttc aaagattgag tagaatagta agagggaaaa
     6421 acggcactcc ggagacgggg tgccgttttt ctgtgctatg ctgctgtaaa tttttctcaa
     6481 aagctcttcc gccacaccga cggcgagagc agcaccagga tggggaaaat ttccagccgt
     6541 cccagaagca tatctgccga cagcaccatt ttggagaagt ctgacagaaa ggaaaagttc
     6601 tccatcggtc ctacccggtt catgcccggt ccgatgttgt tgatgcaggt ggacaccgcc
     6661 gacaccgccg tgccgaagtc catatcgtcc agagagatca gcaggaatga tgcaatcacc
     6721 agtcccagat acaggatcag atagctgagg acgtgctgca atacctccga gcgcaggatc
     6781 ttgtcgtccg acttcacctg gttcaccgag ttagggtgta ccattttccg cagggacgca
     6841 aagccgcttt tccacaggat cacaaagcgc tgcaccttaa agccgccgcc ggtagagccg
     6901 ccgcacgctc cgataaacat gagcagcagg aggatcatct gggaaaacag cggccagttg
     6961 ttgtagtccg ccgtggcgaa tccggtggat gtcatgatgg acgccacctg gaacaccgca
     7021 tagcgaaagc actttcccac actgtgatag atgggctgga tattgagcat gataagccct
     7081 gtggcggcgg cgatgatgca gaaatagaca cgcagttccg tgtttttccg cacctcccgg
     7141 aaacgccgga tgagcaggta ataatacagg gtaaagttca ccccgaacag gatcatggtg
     7201 acggtaatga caccctccag atacggggaa ttatagtagg aaacgccgtc gtttttgatg
     7261 ccgaatcccc cggttccggc aatacccatg gcgtggcaca cgctgtcgaa caatggcatt
     7321 ccgccggtga acagcaggat caccgtgacc agcgtcagca cgccgtagat gaggtacaga
     7381 tacagcgccg aggtctttcc cttggggaca agctttccca cctggggacc gggacactcc
     7441 gcctgcatca gatacagtgc gtttcccgta gacggcagaa tggcgatggc aagcaccagt
     7501 acgcccatgc cgcctaccca gtgggtaaag ctgcgccaga acaggatgcc ccgtggcagc
     7561 gcctccacgt ccgtcaaaat ggacgaaccc gtggtggtga acccggagat gctctcgaac
     7621 agcgcatcca catagtgagg aatgctgccg ctgagataaa agggcagcgc acccaccgca
     7681 gaaaagatga tccacatgag ggctgcaatg accatgccct ctctggcaaa catataccgt
     7741 cgcttgggac ggtacagcgc accgggcagc gccgccagcg ccatgcctgc gcccacgcac
     7801 agaaagatca gcaggaagta gtattcccca tagtacaggc tcaccagcgc cggcacgacg
     7861 aacagtgccg aggacagcag cagcgccatg gagagataat gcagaacaat tcctttattc
     7921 atcccgtcac cgccttaatc cagaatgctt tttagcccgg agaagtggtg ttccttggag
     7981 acgatcacca cagaatcccc cagttcgatc acggcgctgc cgtccggaat gatgatctcc
     8041 cgtttccgga tgatggcgca gatcaggatg ttcttcttca gccggacatt cttcagcggc
     8101 acgcccacca ggtcggggat acgttctttc acccggaact cgatcgcctc cacctggttg
     8161 cccaccagat ggtacaggga ttcgatctcg ctgccggcgg tgctttccag agagcgcaca
     8221 tagctcagca cattgcccat ggtgaggaac ttcggcgaca ccacgcagtc cagccccagg
     8281 tctgacgcca gtccgatgta attttcccgg ctgatcttgg tgaccacctt ggcgttggtg
     8341 ttgtccttgg cgaacaggga aataatcatg ttttcctcgt cgatgccggt gagcgccaca
     8401 aagccgtcca cctccagcac gccctcttca atgagcagat cctggtctgt gccgtcgccc
     8461 cagacgacga tggctttcgg gagccgttcc gccagttcgt tgcagcgctc ccggtccttt
     8521 tcgatcagct tgaccttcat gcccagcgcc agcagctgct tcgccagata atacccgact
     8581 ctgccgccgc ccaccagcat agcagtcttg accttgtcct tcatgaaacc gctggcacgg
     8641 aaaaagcgct cgatgttctt atgggatgcc gccacatgga tgcggtcgcc gctctgcatc
     8701 acgaagtcgc cgccgggtat catcacatcg ccgtcccgct ggacagcgca gaccaggaac
     8761 ttggtcttga tcttcttgta aagttccgcc agggtgatgc ccgacagcca cgggaaatcc
     8821 gggagacggt attccaccag ttccagcttg cccttgccga acacttccac ctttgccgcc
     8881 gacggaaaga ccagcacacg catgatctcg tctgcggtgg aaagctccgg attgatgacc
     8941 atagacagcc ccagatcttc ccggatgagg tgcatctgct ggtagtactc cggcatccgc
     9001 acccgggaga tggtcttttt cgcccccagg cgcttgccga tgagacagca gagaatgttc
     9061 agttcgtcat aaggcgtagc ggcgatgagc agtgtcgcct tgttgacccc tgcctccagc
     9121 tgtgtctccg ccgtagcgcc gttgccctcg atgcacatca cgtccagggt attctggctc
     9181 ttgcgtaggt gcgcctcttc gcagtccacc acagtcacgt cgtgaccctc acgcaccagc
     9241 acagccgcaa ggttgctgcc gatctttccg tttccgataa ttacgatttt catagtatcc
     9301 acccaataaa ttggcaatcg cttgccgcag cttatcatct gttttctcag agcctgttcc
     9361 gcatcttttt cccacgtctt ttcggcagat tttttcgctg actgcgtcaa aatccgcacg
     9421 taaaaagata ctgaacaggc tcttaaaaat gaacaaaatc tattatagca tctttgtgca
     9481 ataatgtcaa cggattatgc agggtttctc ttttttatct cgcacgccaa aaataactct
     9541 gcttacaaat aaaaatgtct ccgggaaatg cagccattcc cagagacaca ctgttttagc
     9601 aaatatcatt ccatgacgga ttcttgccag caccctttaa tgccaaataa tacatcaaat
     9661 aatacatatc tgttgtatcc acttgtccat tgcaatcaat gtccgcaatc acaatttgat
     9721 tttctgtcaa atttgtctcc aaaccggcac cacgataagc cagataaacc atcagttcaa
     9781 ataaatcagt cgtatctgtt ttgccgtctt catctacatc gcctcgataa tccatcattt
     9841 gaaatgatgt cccatcactg caatgaattg ttgcagtacg gagcggtcca tttccactac
     9901 ttcttaaatc aatctggatt tttctccagt cttccaggtt tccggcatat tccacatttt
     9961 ccaaactgtc acaggtacaa aatgtactat attcaatctt ttcaattgtt ttcggaatat
    10021 taactttttt cagagtttca caagcaaaaa aagcattatc tgcgattttt gtcgttccct
    10081 cattcaattc aacctcttcc agattttcgc agaaagaaaa caaattagtg ggaatcgttt
    10141 tcaccgaacc aggaattgtg atttttgtga tttcagattc actaaaagtt gaatttgcaa
    10201 ttccacgaat gtcctctggc aatgttactt ctgcgtattc cttatcataa gatactaccc
    10261 aggaatccac agtgtaaatt ggtccatcct gctgttcttg cacaaaagag tctgcaaaag
    10321 catttgcatc aatctctgtg acactatccg gaatctgaac ttctgtgata tccttgcagt
    10381 tctgaaaagc aaacgcctcg attttcgtaa tcggcttgtc ataagactca ctgggaatca
    10441 ccacaacacc ttctgcctct tctttacagt caacaattgt aagtgttccg tctgtttcaa
    10501 ttcgataaat caaatcaccg taagaaccat atgcggcatt atcttcttcc gctgatacca
    10561 cttgtcctcc aaagttcaag gcacacatcg ttccagccaa aacaatggca aaaatctttt
    10621 tcatttttct catagcatta cactccattc atacaattac tgttgttaca gcacacatag
    10681 caaccataca tttcaacaaa attcattata ctacaaaaaa tatagtagat aaacgtattt
    10741 atacgaacaa gtaggaaata tctgtaaagc aaaacaaatt tgctatcggc aaaaagtcaa
    10801 tccgctatac catccaaaat cacttttttg ccgataaaat ctactgcaaa atgtaaaacc
    10861 ggcattgttt ccggcgtatc ctgtaaggac gaacatggtt cgcccgttag tgcatcactt
    10921 ctacggtctg ctgacatgat aattccgacc gcacttcgtg ccgccttttc tgaaagagaa
    10981 ggctttctcc cattgtctcc tctcatagag gagatgcccg acagggcaga ggagtcgacc
    11041 cgggcgaacc atgttcaccc ctacgagacc aacaaaaaag caccgccggg aaaccaaatc
    11101 tcccggcagt gccgcaatat ttctgctgtt atttttccgc aaacacggtg cttttcagct
    11161 gctgctttgc cgcatcgaag tccacctgca aaacggactc gccgccgata tctgcgccct
    11221 gataggtacc gtccgccggc acacgctgct gcgccatgtc ataggtcgcc agcttcagcg
    11281 gcgtggtgac tgcatagccg tacagagaca gcaccgacat attggtggtc atcttcggca
    11341 gcgcacccat gcacaccgga aacagccgga acgggttgct tttgatcttg cccataacct
    11401 gctccataac cgtgcgctgg cgctcggtac gctcaaagtc ggcgtttccc acataccgga
    11461 tacggctgta ggacagcgcc tgcttgccgt ccagtttcag cttgccgccg ccgtccagca
    11521 ggtcgtcctc ccggtcgtcg cccatgatct cgttgacctc gctgatgagg atctcgttga
    11581 ctgcctccgc ctcccggtcg gagatctcca gctccacacc gcccactgcg tcgatcatcg
    11641 ccgcacacgc cgcaaaggtg atcatcacat agtcgtcgat atgtacatca aagttctcct
    11701 cgatggtgtc catcagcagt tccgggccgc cgtaggaata cgccgcattc agcttggcgc
    11761 tgccctgccc cggaatgtcc acataggcat cacgcataaa cgaagtcatg tagatctgat
    11821 gcgttttgga attcagcgac actaaaatca tggagtctga acgtccacgc tcctcgtccg
    11881 ggtcacgggt gtccgtgccg atcaccagca cgttttttac ataggatgcg tccatactgc
    11941 cgctggtgac agtccggtct ccggtgtcca cccggttcat gcccaggatg cccaccatag
    12001 cgatagcgga atagagcagg aacaccgcca cgatcaccgt cagcacaatg cgcaccagcc
    12061 ggctcagaaa agaaccctgc cgctttcgtg aagtacggtt ccgaccggaa ctctgtctcg
    12121 gcgtactctg ctttggcggt tcccgtctgg tctggctctg ccggggcgga ttctgtctcg
    12181 gcgacgtctg ccgggaggct tcccggcgtg gctgattgta accctgtccc tggggacgct
    12241 ggggctggcg gtaggtctga ccgccgtgat accccggcgc agcctgctgc cttggcggcg
    12301 gactgccctg cggacgctgt ccctggggat agctctgccg tgccgcatag ctgccctgtg
    12361 ccgggcgctg tgcctgctgc ccgtagccgt tctggggata cccattctgc ggatagccct
    12421 gctgcccata gccgttttgc ggatacccgt tctgcggata tccttgctgt ccgtagccat
    12481 tctgcggata gccctgctgt ccgtagccgt tctgaggata cccattctgc ggatacccgt
    12541 ttcggggata gccctgctgt ccgtaaccgt ttccctgact gcgcacctcc tgtgcgttca
    12601 nnnnnnnnnn cgggtatcgt actggtctgt ggagccgtcc gatgcagcgg cacggaaaaa
    12661 catggtctcc tcgccgtctg tgttgttctg tttatgattt cgttccacgc taagccactc
    12721 ctttcacggg aattaccgta tccgcaccgc cagtgctgtc accagcgtca ggatcgcatg
    12781 atagatcagg aaaaaataag ccgtcatctg ggtatatgcg ccggtgacca catggatcat
    12841 cgccagcgaa aagcccagga gtcctgccgc aaccagcagg atcacgccca gcaccgccgc
    12901 acggcgcacc cgtctcgcac ccagcagcag cgcacctgca ccaaagccgg tgtcacccac
    12961 ggtcatggat gcgctgacgc agcccaggtc gccggtctca cgctggaaca ggtggtgcat
    13021 agaggtggga acgatcttta agtaagcctc cgggaaatcg aacagcgccg ccagccgccg
    13081 gagcgaaacg gaacagtcca cactccgcag caccagcgcc acacgctcct gccgcagcgc
    13141 ctgcatctgc cgctttacct tggggtctgc cgtgatgcgc acggaaaaca ttgccgccag
    13201 cacaccgctc accgacagat acagcacatc gccgctgccc tctgccaact ctgcctctct
    13261 ggtcttagtg ggcagtccct cgatgttgtg gttcgccatc atctcccgtc cgccgaacag
    13321 cacacgccgg tttgcgatcc agccgcagag tcccagcccg tcctcacaga caaactcgtc
    13381 taccggccgc agcgtttccc ggtcggggat catctccgca aaggcgctct ggagcatact
    13441 gtctccgtga tgcaccaggc tcgccgcatc caacagcacc tcgtccatct tcgccccgga
    13501 aagcaccttc atgccggcga tctgcaccga tcccttgggg aacaggtcgt tcgcctccac
    13561 cagcagagcg ttggtgtcga aaaagtcgtc cacgctctga taacccagca tggcactgtt
    13621 ggatgccgcc gcctttttcg actcacgctc cagcggcaga ttgactgcca gggcgctcgc
    13681 cgtaccgcag cacgccacct gcaatagtgc cagcatggag cagaaaaagc tgatccaggg
    13741 catcccgaac gccgttccca tgcgaatcaa agtcatgccc acagcgatga gcagcgtcac
    13801 tgcgccgcag atgggcgtca tggtgcggca gagactgtcc gccatatccg tggaatagga
    13861 ataccgcagg atatcgcaca ccccgtctgc cttgcgcaca gcggtgacaa tggggatatc
    13921 gtgcagcacg ccacgggtga gcagttctgc ggtctcctcc tgggagacat aggaaagcac
    13981 ccgtttctgt ccctcccggg agatcacgtt cacattccgc agcgcacggc gcaccatgag
    14041 cagccgtccg atgatgttgc agaacagcgc cagcagagcg cacggcacgt agagatgcac
    14101 cgtccccgaa gaaagggtct ccggcgcaac gacggcgcag actgcaccca gcagcgtggg
    14161 cagcaatggc agcaccgcca tgctgtcgct gtccgcatga aagcggagca gattccacag
    14221 tccgtttttc accgtgggga acatcacgcc cagccccagc agtcccagcg ccagctgaat
    14281 gatgctgtag cctctggggg tgaccgtctg taaaaacgca ctaccgctca gcacttccag
    14341 acacgccaga acagcgccgg caagcgtcag caccagcatg gcgacaaacc ggaagaacac
    14401 cacattccgc aggttttcca gggtcttttt caccaagccc agatcatccg gcttgcgctt
    14461 gtccgcatct tccccgaagg tgtggctgcg cacacgctgg cgaatgggac gaaagctgcc
    14521 ggtctgctgc ttttcctggt aatagtagtc cttttcctcc tcccagtgcc gtctggaggc
    14581 cggcggttct tccggtactg tccgggcatc cgggcgctga aattctccgg taaccgactc
    14641 tttgagataa gtactctccg gtgagcgccg ccgccgtttc cggttctttt ctctggcacg
    14701 ggtggagtcc tgcatctgcc gcacattggg gcgatgcttt tccgccggct cttctgtttc
    14761 cggctcagcc agcatgactg cgccgtcata gccgctgtcg ccgttctccg gataggatgc
    14821 gccatagcgt gccgcaccgg cagccttgac gaaggagaca ttttcccggc ggagatgctg
    14881 ggtgggaaaa cgctcccgca gcaccggagc agcgtcgaca tctcttgccg cctgcgccgc
    14941 cttgtccgcc ttgatgtgag aaatggtgga cttgatatcc accagctgtt cctcgtctcc
    15001 cgcagaaagc tccggcacga cctggtcata gccggcttgt tcctgccgca gcggacggct
    15061 ggaggtcgcc gcattcacca gcttttccgt ctccagcgtc ccgtgggaca agggcttgtc
    15121 cgaggtatgc cggatgccgg agcgcctgcc gtcctcgctg tattcgttca gaatctcttc
    15181 taatgtaaat ttatggtctg ccataagttc actcctgtgg taaaaacgtc acttcgccag
    15241 ccgctggcga actgcttcgt acataaagat gcctgccgcc acggacgcat tcagggagtt
    15301 gacctttcca cgcatgggca gactgagcat agcgtcgcac ttttcgctca ccaggcggct
    15361 catgccaaag ccctctgagc cgatgacaaa cgccgtgccg ccggtgaagt ccgcatccgt
    15421 atagcttgtg cctgcggcat ctgtaccgta gatccagacg ccgtttttct tcagcgtctc
    15481 catggcggat gccaaattgg ggacacgtgc caccggcagc cagcttgcag cgccggcaga
    15541 ggtctttccc accgtcagcg tcagcgatgc gctgcgccgc ttggggatca ccacgccgtg
    15601 tgcgccggca gcctctgccg tccggatgat cgcacccaga ttgtgagggt cctggatgcc
    15661 gtcacagagt atcagcagcg gcggttcgcc cttttctgcg gcacgttcca gcagttcttc
    15721 cagggaaaca tagatgccgc acgcaccctc ggcagccacg ccctggtgga ctgcgccgcc
    15781 ggtgagctgg gacagcttgg actcggtgac cactttcacc gggatgccct tttccttggc
    15841 gagccggatg atctcctgga gaccgccgct ctcctgtccc acatagatca gatcgatctt
    15901 ggcatcactc cgcagcgcct ccagaacggg gttgcgtccg gcaatgcggt cgttttcggt
    15961 ctccggtgca tccgccggct tttgtccccg gcggggctgt ggttttctct ggctcatatc
    16021 aaatcctccg ttatctctct gcgctgtgtc caaaagtaca gcacagcttt tatttttgca
    16081 cgattctatt tattatagca cacccagaga gaaaaaacaa gaattttcac gggattttca
    16141 gatttttctt tccgccggga gctgtcacag cagcagcagg cacaccgccg ccgtccccag
    16201 gcagctgagc agcaccagca ggacagtacg cagccagaac cgtttttccg ccttgtccgg
    16261 cgcagcctcc cggagcaggg catctgcatc cgcacgggac aactgctggg gcagctgggt
    16321 cacgccgggt ttgagataaa acacggcacg ttcaaaatac aggctgtccg gatggttcac
    16381 ttcgatgatc tttttctgta cgccttttac catttcttct gtctcctcct gtttccgccg
    16441 tgcagtgtcg cacgagcttt tcacatctgc acaaactttt tacatctaca cgggctttta
    16501 acaagagtat gcgcacctcg gaaaccgttc aaacagaaga accccggttt tccacaaaaa
    16561 ccagggattc gaccgtatat tctgtggaaa actctgtgga aactgtggaa aacaagcgct
    16621 ttttccatct ctgtccccga atgtggaaac tggtggagag ttttgccgga cggctgtctc
    16681 agacgcctgc cgtcaggggc atcaccggcg catcggtgcg aaacggcgcc gccggaatac
    16741 cgttcgtccc gaacagggag accggtccgt agttcaccca gttgtaccgt gccgccacgg
    16801 gtgcatccac gccgtccgcc gtgagccgca cggtgttccc gtccaggaca gcctttgccg
    16861 gatgatacgc cccgtctgcc ccggcgatct caaagcccac gggttcgccg tgccatacca
    16921 tcccctgttc ggcgtgggaa aagaatacgg tgagggtgtc gccctctgcc gcaaagctgc
    16981 ggtagatagg accgaacgcc tgcaattccg tttcgccgta gaccagccgc cgtgcctgga
    17041 gcgccaggcg gtgtcccacc gggtgcttgt ccacagggtg gatgttgcag tactcgccgc
    17101 agtccaggat cacggcaatg cctgtgtttt tcaccgtctg atacacccgc atctgcgcct
    17161 cacgcagctt gctccacgcc ccgttttccg gggtgtccgc ataggtgtgc atgggcagct
    17221 gcaccaggag aaagggcagg gtctcgtccc ggaaatcctg gcgccaccgg gcaatgagca
    17281 tcgtcagcag tgtaccatag gtgtcgggtc ggatctcgtc gttttcgccc tgataataga
    17341 aaaatcctcg gatggtatag ggtgtcacct gccgcagcat ggtgtggtac atcccggcag
    17401 gacggtacgg gcttttgatg cccatcggac cgggatagcg gttttcgccg cagcgccgga
    17461 taatctcgtc ccagggcata ttgccgtcct cctggtagca cttctgcatc cgcttttccc
    17521 aggcggtgtg ataggtcaca tattcgtcat aggcggcgat catctcctcg tcggtctttc
    17581 cggcgacggc atccgcatag tcctgcaaat aggggtgtcc ggcagtgtgc gcccggagat
    17641 cttcctccgg cagccagcag ctcaccgaag agccgccgaa attgcagccg atgatcccca
    17701 cgggaacgcc cagggtctgc gccagctcct ttgctgccag atagccggca gccgaccagg
    17761 tgccgctggt ctccgtggac ggcagctccc agtgggcggc atccatggca gctttgtaag
    17821 cgtcgtcccg gaaggtattt ctcggcacgg aaaaataccg gacgttggag tctgcgcact
    17881 gcgccagttc cgcagcgccg tttttggcat ctttcagcat gaactccatg ttggactgtc
    17941 ccccggtgag ccagacctca ccgaatgcca catccgtgaa ggtctgttcg ttcaccgtca
    18001 tggtacagcc cgttccgccg ggcatgggcg gcagtgtcac gcaccagctg ccgtctgcat
    18061 ctacagcgcc ggaaacagta cagcggcgct ccgggacgga gaccgtcacc gtctcgccgg
    18121 gcgttcccgt gccgaaaacc gcaatgggct ttccacgctg gagcagcata tggtctgtaa
    18181 aaatggttgc acattgtatc ataatggatt ctcctcgtat tgtgatggtt ttataggcaa
    18241 caccgcacaa agcttgtgcg taagatgcgc agccagattt tttggcagat tgcgcagaaa
    18301 tttcgcaggc atactgtcgt atgtcaaggg atttctgtgc aatatgacgg aaaatcagca
    18361 agcagattgc gtgcaaagcc gccaggcggg ctttgtgcgg tattgccttt attttacgat
    18421 gatctcgcag tccggctcaa ccttgctgat gcacttctgt gcagccttca ataccttgtc
    18481 gaaatccgca tcgtcggcct ccagcgtcaa tttctgggtc atgaaactga tggttgcaga
    18541 ctttacgccg tccagtttct gaattgcagc ctccatcttt gcggcgcagt ttgcgcagtc
    18601 cagatcattc agcttaaata cctttttcat tttcgtttcc tccatgcaat ttcgtttgaa
    18661 cgattgttca tatgttcatt atatacaatt acaccgccgt tgtcaaccct tttccgcaaa
    18721 aaaagcgaat tccagcccat cttcaaagaa acgaaagttt tcccacgact tcgcataaaa
    18781 aaagagcgca tctgcatccg atacgctctg ttctgcaagt attctgttat gaaatcgagg
    18841 gtcatccggc aggcgtgtag tcctcccggg taagcgccgc ctcggaattg tagaattcca
    18901 ccagggcgtt gttgtcggta tactcgccca gatacggcgc ataccaaagc ccgaagaacg
    18961 accacaccgc attgtcccgg aacatggcgt tcatatccgg gagattgctg cattcgctga
    19021 tagcgagcag cttgccggag gaaaccatgt tccgcagcgc cacgtactgc tcatagcggc
    19081 tgccgtaggt ctcctccttt tccacgtaca agtccaggga cgcaatgtca tactggctgc
    19141 tgtccaccag aaagctgctg ctctgtccgt tccacaccca gagcagattg ttcagatggt
    19201 gatattccgt catccgggtg tacatcagat tccagagcca ctgataggtc tctgccccgt
    19261 ctgcgcccca ccagtaccag ccgccgccag cctccgggag cggacgccac agcaccggta
    19321 catcggcatc tgccagaggt ttcagcgccg cagaaatgcg gtcgatgtcc gagatcagcg
    19381 catagcagtt ctgggtgatc tcgccgttgg agaccatctt tgacagtttc tcctccgact
    19441 tgccggcaac atccgcctcc gttaccgccg ttttcaagtc aaaggagggg gccttttnnn
    19501 nnnnnnnacg ccgccttctt ccgcccacgc caggctgtct gccacagcgg tggcttttgt
    19561 ctcatcgccg ccgtttggcg aataggcgtt catatcggca aagcgaatca gcggatactt
    19621 tccggtgatg ttgtagatct cgtccagttc cttgccgctg ctgtcggaga catactgccc
    19681 ggtcacgatc ttctgcccgt agttctgctg taagaactgg tatagctgtt tcgtcttttc
    19741 cgtggcggcg gcattcaccg gcgctgcggc gatctcggat gtgccgtcat acaggctggt
    19801 gttgttcacc agttccacgc agtcgatatc cacgatgccg tccaccggaa ccacagacag
    19861 ttcacaggcg ccggcgctga ggaagatgcc ctgtatcttg acacgcataa aattatccgt
    19921 ggcatctgtt ttcatggtat agaccgactc gccgttcacc aggatcttac aggtagaggc
    19981 tgcgcttgca cccactacca cggtgatatc gtagtgctgg gtcgccggga tgtcggcatc
    20041 cagcaccagg ctgttctggt ttttggcatc cagaccgctg aggtagcctg tcccggaata
    20101 gcccagccgg ggcatcatcg ccaccgaaca gcctgtggga acagcggcat cctccgcctc
    20161 cagtttcatg gaaacatcct gccgggtgat ctccggcacc tgcagggcaa cagacgggtg
    20221 ctgcgccgca gccgaggtct cttccgttgc cagctccgtg gtaccggtgg caacgtccgt
    20281 ggaaagtttc ggggattcct gttcaatttt tgcgcaccct gtcagcagca aaccggctgc
    20341 cgccagagca aacgcctgct cttttctcaa aattgctcca tctcctatga atacaaatca
    20401 atttttaaga aagtccccct tgaaaagggg gatgtcagcg ttagctgaca gggggattat
    20461 cccgactgca ctttgtgcat cctcagcaca atacacaccc tacggatgcc ttttcaagag
    20521 aggcagttat aaaattgatt tgcgtttctc ctatgaatgt ttcgagcttg ctttctctgt
    20581 gcgcttacgc tttcagacgg tacgccatgc ccttttccgt ggggaatgtg agcaaagccc
    20641 cgtccatctt ggcatccaat gcgccggcat cgctggacac gctggtcacc gtgttggcac
    20701 ggacggtgca gacattgccg ctcagcgaac ggatcactgc ggattccagc ttgccctcgt
    20761 gccaggtcat atccacctca aagccgcctc ttgcacgcag accgctgatg ctgccctccg
    20821 gccaggattc cggcagcgcc ggcagcagat tgatctcgcc ggaatgactt tgcagcaggc
    20881 actctgcgat ggcagccgtg ccgccgaagt tgccgtcgat ctggaacggc ggatgagagt
    20941 ccagcagatt cgggttggta gaccacgcca gcagctgctg caaattctcg tatgccatcc
    21001 ggctgtccag gagtctcgcc cacagattga tgatccatgc acggctccag cccgtatgac
    21061 cgccgccgtg gatgagccgg cggatcagtg tggcacgggc agccttgccc agttccggcg
    21121 tgtgatacgg cgtgatgagg tctgccggat agagggcaaa caggtgcgag atatggcggt
    21181 gaccgatctc cacctcttca taatccttcg cccactcctg gatctgaccg tacttgccga
    21241 tcttcagcgg cggcagcttt ttcagcagac cttccagctc tgcggcgaag tccccgtccc
    21301 ggtttagcag cttgctgctc tcgatcacgt tccggaacag caccgtcaga atttccgtat
    21361 ccataaacgg accgctgcac agacagccct ttgcgccgct ctcggtttta taggtattct
    21421 ccggcgagac agacggacaa gtcaccagtt cgccgtcctc gttctccgtc agatagtcca
    21481 cgaagaacag ggcagcctca cgcattgcct catagtgctg atccaggaag tccaggtcct
    21541 gggtgaactg gtagtgttcg aaaatgtgca gacacagcca agccgcaccc atgggccaga
    21601 tggtcgccgg catccacaga tcctggggtg ctgtgtcgcc ccagatgtct gtgttgtggt
    21661 ggcagacaaa gccacggcag ccgtacatcg cctgggcagt ctcccgtccc ggcgcacgca
    21721 tccgctcaat cagatcgaac agcggcaggt ggcattccga caaattgcag acctctgccg
    21781 gccagtagtt catctgggtg ttgatgttga tggtatagcg gcagccccaa gccggccaca
    21841 tatcctggtt ccagataccc tggagattca tgggctgcgt tcccggacgg ctggcggaca
    21901 gcatcagata tctgccgtaa ttgaagtaca gcactgccag cttgctgtcg tggatctgga
    21961 gcttgcactc cttgtggtcg ccctcgtccc cacgcagccg catgagccgt tcatctgtgg
    22021 gcagctcgct gccgccttcg ctgttgtccg gcagggtaaa gcgcacccgg cggaactttt
    22081 cctggtattc cgtcagatgg cggtacagca gttcctcata gctgcagtct gccgcataag
    22141 aggcatccat ctttgccgca tcctcgtact gttccccgtg gtaaaagctt gtgccgacgc
    22201 tcagcagcag ggtcacttcg ttcgcaccct gcacttccag cgcaccgcct acggtgcgga
    22261 tggtgccgcc ttcagaaaat ccggtcagcg ccgccgcaaa gaagatgccg tttcggctgc
    22321 cggtgccgcc gtcatagacc agcatattgg gatagtcggt acacgggcgg cagtcgtcgt
    22381 agtaatcgtc tctgccgtcc agagtggcac gcaggttcac catgccgggt tcgcttgcgg
    22441 caatatgcag taccagcacc cgatccggtt cggagacgaa cgcctcacgg acataggtga
    22501 tatcgtttgc cgtaaagcgc acggttgcca gagcgtgttc cagatccagg ctgcgctgat
    22561 actgctttgc ctttccggtg aaatccatgt gcaggttcaa gtcacccagc ggcatatagt
    22621 gccgagaatt gggtgtcacg ccctgtaatt tttcgaaagt gatcttttcc gcctcgggga
    22681 tgcgctcttc ccggagcagc tttcgcacct cttccagacc ctcacgggca tccgggttgt
    22741 tccggttccg ctttccgccg gaccagacgg aatcttcatt cagtttcagc acctcgtctg
    22801 ctgcgccgcc gaaaaccatt gccccgatcc tgccgtttcc gatgggaagc gcctggtcaa
    22861 aattttccgc tggcttttca taccagagca tcgtttctgc atccatgtca aatcctccgt
    22921 ttttccggac agtcccgtcc cgttttctcc tcagaaagct gcatcagccg gcgcagcatt
    22981 tttccgcacc gcacccggtc gctttccgct ctcgccgcat ttttctccgg agcggtcaca
    23041 cgcaccggat acggacattc tgtcgttttt ggtttctgca tcggctctcc ctcccggaac
    23101 agtctatgca gctgtttttg ataaattgat tttcgaaaaa gtattgcaaa tacaaagaaa
    23161 gcgcagtatt aacgctgcgc tgtctccatg accagacctg taagccgagt tctgtcgtgt
    23221 gtgataattt atctggtctg tgagtcacct cacagctcaa gccgccatta cgccgctgcc
    23281 caccggacca atgttgcggc agcgtgtacc aatagacgtt gcatcggata agg
//
LOCUS       DAWENO010000013        22887 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_930_length_22887_cov_13.610897, whole genome shotgun sequence.
ACCESSION   DAWENO010000013 DAWENO010000000
VERSION     DAWENO010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 22887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22887
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_22887_cov_13.610897"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3321..3330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18271..18280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5519 a         5724 c         6018 g         5606 t
ORIGIN      
        1 caaaaagaca gtcaaggcac tggagatcaa gccggaggtc gttgatccgg aggacatcga
       61 gatgctccag gatctgatta tcagcgctgt caacgaggca gtgaaccagg tagaggctac
      121 cacagagaca gagatgagca agatcaccgg cggtgtttct ctgccgggtc tgttttgatc
      181 ggtaagaatg gctggataca atgcgcttcc gctggagctt ctggcggagg agttcgcaaa
      241 gctgccgggc atcgggctga aaacagcaca gcgcctggcg tattccatca tctcccgtcc
      301 ggaagaggac gtggagcggt tcgccaacgc actgcttgcc gcaaaacggg acatccatta
      361 ctgcccctgc tgtcagaatc tgacggatct ggagatgtgt cccatttgtg cggacgatga
      421 gcgtgaccac agcgtgatct gcgtggtgga gagtcccaag gatgtcacgg cgctggaacg
      481 caccggcgaa tacaccggag tataccatgt gctgcacggt ctgatttccc ccattgacgg
      541 ggtgacgccg gacaagctgc gcatccggga actgctggca cggctgcaga gcggcgaggt
      601 acgggaggtc attatggcaa cgaaccccac cgtggagggc gatgccaccg ccatgtacct
      661 ggcgcagctg ctggaaccgc tgggagtcaa ggtcacacgt ctggcattcg gtctgccggt
      721 aggcggctcg ctggaatatg ccgacgaggt gacgctgttc aaggcgctgg aaaaccggac
      781 agcaatgtga gcgctgtttt tacaagaaat attcgccctg ttcgtggggg attctccgca
      841 ggcagggcga ttttttatca tgtgggatga gagacttctc agtcaagcct agcgactccg
      901 accgcactgc gtgccacatc ctctgtagag gaggctctct catagaggag atgcccgaca
      961 gggcagagga gtcgatttgt cccttttgaa aaaggctcac cgtaggggcg aacattgttc
     1021 gcccgagact cctcagtcaa gcctacggct tgccagctcc tctgtagagg agatgcccgg
     1081 tagggcaggg gggatatatt gtcctaggca taactcccga aacccgtccg gcggaattcg
     1141 ctcgttcctc gctgtccgcc tttggcttgc aaacgccttt gtttgccttt tcctgtgtgt
     1201 gcagtgttgg cattctgggt gtttgttctg ggcgtggttt ttgtttttat atagcgtttg
     1261 ccacgcaaaa agtctcctct tatagaggag atgcccgaca gggcagagga gttgccttgt
     1321 ctcccttgaa aaggcgcccg cagggtgtgc cttgtgctga gatgtcagcg tagctgacag
     1381 agggatttgc aaaaatcacc cttttttctt tactgaaaag aaaaaaagaa gcaaaaaaag
     1441 aaaagcttat tgcctaccct tttataaaaa ggcgatgtat cacacgactg gcagtgtgat
     1501 gcgattagat tacgcacaac aaaaatcccc cggaggggta acaggcggct gctgccgccg
     1561 taacactgcc ctttttgaat gttaaattgg ggcgaaaggt cagcggcgac ccgccgcata
     1621 tgatagaaag gtacaagtac aatatgatta ctgtttccaa tgtcagtctg caattcggcg
     1681 gcgacacgct gtttaagaat gtagaccttc aattcaaccc cggcaactgt tacggcgtca
     1741 tcggcgcaaa cggtgccgga aaatccacgt ttctgaaaat tctctgcggt cagctcaagc
     1801 ccaccaccgg cgaggtgacc atccccaaaa atctccgcat gagcgtcctc aagcaggatc
     1861 acttcgccta cgacgcatac actgtcctgg ataccattat catgggcaac cagcggctgt
     1921 atgacatcat gcagcagaaa gacgctctct acgccaagga ggacttcacc gaagaggacg
     1981 gcatcctggc atcggagctg gaggcggagt ttgccgaact ggacggctgg gaggcagagg
     2041 caaacgcctc taagctgatc cagggtctgg gtctgccgga aagtctgctc tatcagcaga
     2101 tggcggacct gtccggtaac gaaaaggtga aagtcctgct ggcgcaggcg ctgttcggca
     2161 atccggatat catcctcatg gacgagccga ccaaccacct ggacattgac gccatcgcct
     2221 ggctggagga ctttctgtcc gagtatttcg gtacggtcat cgtggtttcc catgaccgtc
     2281 acttcctcaa cgacgtctgc acccacatcg tggacatcga ctataccaaa atcaaaatgt
     2341 acgtgggcaa ctacgaattc tggtatgagt ccagccagat gatgcagcgc cttatcaaac
     2401 agcagaacaa aaaggtggag gataagatcg ccgagctaaa aacctttatc gcccggttct
     2461 ctgccaacaa gtccaagtcc aagcaggcga cttcacgccg gaaactgctg gataatctga
     2521 ccgtagagga actgccggca tccagccgcc gctatccgtt tatcggattc gatatggatc
     2581 gggaactggg caaggaagtt ctccaggtca acggcatttc caagacggtg gacggcgtga
     2641 aactgctgga caacgtcagc tttaccctgg gacgcaccga caaggtggcg tttatcggcg
     2701 agagcgagca ggcgatcacc atgctgttca agatccttat ggaagaggaa gagccggacg
     2761 agggtacgtt caagtggggc gtttctacct ctacttcgta ctttccggtg gacaattccg
     2821 cttatttcaa cgacaatgac gactccatcc tggactggat ccggcagtat tccaccgcag
     2881 acgagaccga gaccttcctc cggggattcc tggggagaat gctgttctcc ggcgacgata
     2941 tctataagcc ggtaaaggtg ctgtccggcg gcgaaaaggt gcgctgtatg tttgcccgga
     3001 tgatgctgtt ccactccaac gtgctgctgc tggacagacc taccaaccac ctggatctgg
     3061 agagcattac cgccgtcaat aacggtctgg tgaacttcaa gggcgttgtt cttctgtcct
     3121 cccacgacca cgaaattctg gagactgtgg caaaccgcat tatcgagatc accccggacg
     3181 gctgcatcga ccgccagggc acgtatgagg aatacctggt ctggaaaaag gaaaacggga
     3241 aataaggata aggctgtttc aaatcctttt gtataacccg gcactttccg gacatactga
     3301 aagtgtaccg cccggcgcat nnnnnnnnnn gtaccaactg cgattctttc gtaaagcgca
     3361 gcggcgctga gggctgagtc taggaattcc gcctgtgcgg tgaaagccgc cgggaaaagt
     3421 gcggcaccgc atttttcccg gctacatagg gctgggcgga agaaatatcc ggctggtttc
     3481 gtcaaactgc acaaaaaaac gggtgcataa aattgcaaaa cggcaaaaaa aggggaaatc
     3541 ccttgtatat tgcatggttt tcgggtataa taagagagta ctcaagttcc gaaggaggta
     3601 gaattttcat gtatgataaa acgatgacgt tttctgtcag tgatgaaaaa gagattgaga
     3661 tgaagaaaac gctgatgctt gtatatgatg cactcacgaa aaagggatat aatccggtga
     3721 atcagattgt aggctatatc ctctccgagg accccaccta cataaccacg tataataatg
     3781 ccagaacgct gattcgtcac attgaccggg atgagctgct ccaggcattg gtaaagtctt
     3841 atctgaatca gtgatccgga gcgcatagaa cggatataca ataacgcatg gaaaagccgc
     3901 accgcaggtc tgcgtacaag cagaagacgg tgcggctttt tgctgtcctc ccgtaaaaga
     3961 cgattgtgtg cgtcacgctt tatctacaga ccattccata aattcaaata agaattgcac
     4021 agtacatttg gtttgtttta tacattccgt caattgacaa aatatgcaaa tcatggtaca
     4081 ctgactataa gaaggaaaat cgaccaaaaa gaaccaaaca cacaaaaagt gggatgccta
     4141 tttgttgaac tatacaaatg gtggcaaaaa ccgttgacgt aattatataa aagtgctata
     4201 ataagaagag aagatttcgt gcgttttccc actttgcatg gagaggatgg atatatcatg
     4261 aaacgcatcc ataaaaagat actggcaatt tttacggcaa tattgcttgc agtaacaaca
     4321 tttgtgcttg caggggaaaa aacattttca gcgtgggcgg tggacgatct tgagaccgtc
     4381 gatgagacca catcccagac agaaggaaac atgggagata tttatatcgg aaacgtggaa
     4441 attccgctgg atcagctgat ccagaatcat tataaggtca caattccggt aactatgccc
     4501 aataataccg gtttttcgat cttacagttt ggcgtaaagt gggacgtaac aaaaatgtcc
     4561 gtccaaggcg cacgctgtac cggcgatatt cagctgcctg tgctgacgtt ttcggatgac
     4621 aagcggcaga tctggatgat gttcctggag gcggactgca aggagaccaa cctctgctcg
     4681 ctgacagcac tgctcaacag cgatgtcaaa gtgggcgata cctttacatt agagggtgca
     4741 tacagcgact atgccggaaa cccggcgcag tatacagacc cgtccatgaa agcgcatgat
     4801 gtcaacatcg tgtccggctc gatcaccgtt gtggacgatg tggcatctga ggtagacctg
     4861 gagctgggca gcgtaaaggt cagtatgcgt gatctggacg aacatgacta catggtagaa
     4921 gttccggtca cagcatccaa aaatacggga ttctgtgaca tggtctttgg tttccgctgg
     4981 gacaccagca aggtcaccgc agagccgccg tcgggagata ccccggaagg gctgtctctg
     5041 atcccgacca ttgacaacaa aaacggcgtg gggtggattc atgtcatctc cagcacgccc
     5101 tataccgggt cggagatctg taccctgcgg tttacgattc cggacaccgc aaagcccgga
     5161 cagtcttttg ccatcacggg ctgtatgcag aacggaacac agaccgcatc ggtcgcaaac
     5221 cgttccggtg cggcaggaac gcttaccata caggacggca agatcatggc ggcaaccaca
     5281 caggcagcca atccctttgc attcggcaag gtcatactgc cggagatcaa tgtggcaatg
     5341 gaggatctgg agtccagcga ctatctggtc tctgttccga ttaccattac cgagggcagc
     5401 tgctttaccc agttggcatt cggcgtgaag tgggacacca atgatctgag tcttgtcagc
     5461 tgtacctgtg acgacaccaa aaatctgggc atgatggagt cctattatga aaacgatgac
     5521 ggcgtatggc tgcaattttt gtatcgtggg gcgtatgatg cctttaccgg ttcggaactc
     5581 tgtacgctga cgttccgggt gcgctcggat atttctctgg gcgacaagat cattctgacc
     5641 ccttcggaca ccagcgatga gggcgacagc atgatgatcg tcagcaccaa gggaacatac
     5701 ggcgatctga atatgcagaa gggttcgatc aagctggtca gccccgatga gctgactgct
     5761 gcggcaacgg tgaagatcgg cgatgtggaa atcaccgaat ataatttgtg gctgaaaagc
     5821 tatctggtgg atattcccat tacattgaat cgaaacagcg gcatttccgg catctctttc
     5881 ggtgttacat gggacaaggg cgtagatcac gccagtgtgc tggacagtac ggatatttcc
     5941 gtcctgggcg taaacagaga ctttaccaca gacccgaacc aggcgtggct ggagttcaac
     6001 tgtgtagacc cgtccagcgg ctatgtctat tgggacaaga acctgggcgt tctcaagctg
     6061 cgcctcagtg aagatctgaa agcaggagat gtgatcaccc tttccggcgt gagcctgtct
     6121 gcaggcggcg tggtggcgac ggtggaatcc gccgacggct cgctttcgtc tccggcgctg
     6181 gtctccggct ctatccgcat cctgccaaat ccggacacca cggaaaccac accggcagag
     6241 accacggaaa ccacaacaga aaccaccacg gaaaccacaa cgacgacaac aactgccata
     6301 gagacaacca cttctgcgga agaatcgacc acagaaacca ccacttcggc ggagacgacc
     6361 acctcggaaa gccgcaccgc cgaagagaca acgacggaaa ccacagtgac aacagaaact
     6421 gcgacagaat ccgagacgac aactacagtt tcggaaacga ctgcgccggc aactaccaca
     6481 agtcacagca caacggttcg tgggaatcgg ctgaaccgca cgacgcttac cattcgtgcc
     6541 ggacagacca gccgtctgga attctatccg gcggacggca gcggcgagga ctgcgtctgg
     6601 ctcagctctg accccaatgt ggtaaaggtt tctcccaccg gaaatgctgc gcagatcgaa
     6661 ctggtcggcg gcagaaccgg cacgacggaa gtcaccgtac tgtatgcagg gcaggtgtat
     6721 tcctgccagg taacagtcct ggcggcagcc aatcagtctt ccggcgatgt gaacggagac
     6781 agccagaccg atctgagcga cctggtaacg ctgaataaga tcctggtggg acaggtcacc
     6841 ccggacgggg atgagagcca gagcgccgat ctgaacggag acggctatgt ggacagccgg
     6901 gacgcactgc tgctgatgca gctgttgatg cagcgtggac tgctcagcta aggcggcgta
     6961 taaaaaaaga ttttatcaca cttgtttttc tttatttttc tcttttcaat ctgtgaaaaa
     7021 gcgatgcagg gcttgcacaa aagcctgtcc tgcaccgctt tttcattttg ccgtgttgtt
     7081 tccatgcaat ttatgagcgg caaagggtat actgtgattg tctgtagcat cgcagccggc
     7141 gctgtcgttg acttttcaaa aaaattgtgg tacactaaat aaggtaaggc aaacgcattt
     7201 tgcctgcaaa ttctgtggaa ggaggaatgg ctgtgaatca gtttcgtgtg ggactgcggc
     7261 ggctgacatc gcactggaaa ccgctgctgc tgtttgaact gctgtggaaa gtgttgactg
     7321 tgctggtgat cgccccggcg tgtgccggac tcatccagct tgctattcgt ggcgcaaagc
     7381 tgaactatct caccacatcc aacctggtga aatttctccg ttcgccctgg accattctgc
     7441 tgatcgcagt gatccttctg ctggcggcgc tgtataccct gttcgagatc tcggcagtct
     7501 gtacctgttt ccgccagccc cggcagcaag gtgttcgggt cacgctgggg catatggtgc
     7561 gctccggact gcattccctg gtgcattttt ttcggggcgg cggaccgctt ttggtgctgc
     7621 acctgctggt gctgatcccg ctcatgcagt tttcggcgac ttccggtatt ttcaccgcaa
     7681 tgggaatccc ggattttttc gcctattata tgacgaaaaa ggaatttctc ctgcccatct
     7741 atgtggcggt gatcgtcctg tgctgtctgc tctccattcg ctggatcttt tccagcgtgc
     7801 tgtttaccca gaaccagtgc agctatcgca gtgccagagc cgccagcgcc gagctggtac
     7861 gtggacgttt ctggaaaacg tttttctccg tgctgttgtg gaactgctgt tattttgcag
     7921 tgctgctggc atttttatgc cttatcatgc tgggcgtgct gatggtgatt cacgggacgg
     7981 gaaacagcaa tgtcatcaca tcccaggcga tgcgcatttt gaaactgctg atacagctgg
     8041 tgctgtggag cttttcgttc ttcgcaacgc ccatctgcat ggcgcatctg acggcactgc
     8101 tggaaaagcg ctgcgcccag ctgccagagg tggccctgcc ggaacccatt cctctgagcc
     8161 ggagcgcaaa gcccattcgc cgcagcacag cgctggtgac tgccggctgt ttcacggtgg
     8221 cagccctggg gctgaacctg tcctatgtgt acagcgtgtt caccggcaag gcgaatttcc
     8281 agctgacgct gttccagaat cccaccgtta tggcgcaccg gggactgtct gcggatgcgc
     8341 cggagaatac gctctatgcg ttttccgatg ccatttccgt aggtgcggat ttcattgaac
     8401 tggatgtgca gcagaccaag gacggcgttc tggtggtgat gcacgactcc aatctgaaac
     8461 gcactaccgg cgtaaacaag gacatctggg atgtgaacta tgcagacatc caggatttgg
     8521 acgccggcag ctggtttgac ccggcgtatg ccaacgcacg tatcccgacc ctggaagaga
     8581 cactgcaatt tgtggacaaa cgggcaaagc tgaacatcga gatcaagccc acaaagcacg
     8641 gttcggatac cctggagcag gatgtggcga atcttatcac ggaatatcac tacacggatg
     8701 cctgctatgt gacatcgttt tcctatggtt cattgaaaaa ggtgaaagag gtgaatccgg
     8761 atattcgcac cggatatctg atgagcgtgg catacgggca gttttactcc ctgcaatatg
     8821 cggacgcatt cagtctgaac aaggtgttcg tcaccagcca ggtggtcaat gcagcccatc
     8881 agcagggcaa gcagatattc gcctggacgg tgaacagcgt gtcggaggtg cgcagtctgt
     8941 gcaatctcca cgtggacagt atcatcacgg acgatccggt catggtgcag aatgtggttt
     9001 cacgggacag caccagtgag accctgcggt cggtgctgga ttattttatc aattaggggg
     9061 aaggcgcagg ggtggatacg gtttgtctct gcaattgatg cggcttttat ttgacatttt
     9121 ttgtcgttta tggtatacta aaattctgag ggcataccaa taacggtagg cggtttgttt
     9181 ctgctcccat gattttataa tcgatgggag gtgatgcgtg gtgatggatg tcatttcctt
     9241 tatcgtaagc attctttgca atgtttttcg tgcgattctt gcgtacttaa catacaaaaa
     9301 gaaataaccg ccccagcttc ccaacttagg cggttatttt ataactaaaa cttgatggga
     9361 gcagaccgtc tactggtatg ccctctcttt atctctatta tacgctagaa tgcagtattt
     9421 gtcaagtgcc tttttgttgt agcacgcagg ctgtttccgt agcattttga aaggtttctc
     9481 ataggggcga acattgttcg cccgagactc cttagtcaag cctacggctt gccagttcct
     9541 ctgcagagga gccaaaagaa aatctcctct tatagaggag atgcccgaca gggcagagga
     9601 gtcgatttgt cccttttgaa aagagaaatg tagggggcga tgctcgcatc gccccttaat
     9661 ttctggaaac actgtcctag ggacaactcc caaaaaccgt tcggcggaat tcgctcgtgc
     9721 ctcgctgtcc gcctctggct gtgcaaacgc ctttgtctgc cttttcctgt acttgtaatg
     9781 ttgattttca agatatttct tgtgcagatg gtttatgttg ttattatggc gtttgccacg
     9841 caaaaggtct cctcttctag aggagatgcc cgacagggca gaggagttgt cttgccaata
     9901 agttctccca agagttattc ttcttttttt ctttactgaa aagaaaaaaa gaagcaaaaa
     9961 aagaaaagct tttcgcctac ccttgttctg caaggcaacg tattacacga ttggcagtgt
    10021 gactcctcag tacgatacaa caacccgccg cattctgaaa ggaaaaagcc gctatgcgcc
    10081 tattcaccgc tcagctcccc acacatgaaa aatccctgga acacgccgcc gcctatgcgc
    10141 tccttaccca cgcagtagag gtcatttacc atatgccctt acccgatatc gcacgggatt
    10201 ctcatggaaa gccctatttt ccggcgcacc cggagatctg ttttaacctc agccactgcc
    10261 gtggactggc ggcgtgcggc atctctgatg acgtcctggg ggtggactgc gaatttatcc
    10321 gtcctctgcg gcccggcgtg ctgcaccgct ctttttcgcc ggatgaatcc cggtttgtgc
    10381 tggaaagccc cgtgccggac gaatcctttt tccggctctg gacgctgaaa gaatcctttg
    10441 tcaaggcgct gggaatcggc atttcctatc cgctccacac ggcggttttt gccctggacg
    10501 gcgaaacggt cacggcgcat ccggacggct ggcagttccg gcagttccgg ctggaaagcg
    10561 gtcatgtggt ctcctgctgt gtctcgcccg gggatacgct cccggcattt ccggaacata
    10621 tcgcaatata acgccgcctg tgggtgtcgc ctttgtaaaa agtcacccat agcggctttt
    10681 tttctttgtg tagaacgcca aaaatcgcca aatacgctga atagaaatgg aaagtttaca
    10741 tttcattatt ttcagtatga tataatggac aacaagagga aataccagtt ctgccgaaag
    10801 aggcagatcg tcaggaaaag gagaagaatg aagatggaga aaaccaaaca aagacttgcc
    10861 ggggataccc acagcggcgc cggtctgctg agacggatgg cggcggcagt ggtgtccgtg
    10921 gttctgctgg tcaccgggct gtcgctgcac agcgtggtaa atgcggaaac caccctgcct
    10981 gtgctggaga ccgacgtgac ccaaggcaca tcggacacca cgctggtggg cataccgggt
    11041 acgtatatcc agcagatcag cgaggcgctg gaactgataa acagttaccg gaaagaggcg
    11101 tgcgacaacg gctatcccaa tcccagcact ggcgcagcgc tgacctcctc ggactatgtg
    11161 ccgatccagt ggtcggatga cctggaatac attgcacgaa tccgtgcggc agagagttct
    11221 cagaccatgg gtcatgtccg cacaaacgga aacagctgtt tcaacatcta ttcgcctaac
    11281 gggatgcgct cgttcggcga gtgtctggcg tggaactaca gcaagaacgt gctggacggt
    11341 atcgaacagt ggtacgagga aaaatcggac tatctgaaca acaccggcgg tgtcaccgga
    11401 cactatacca gcatgatcga cccgaaaaac acccgggtcg gcatcggcac attttatacc
    11461 aagctggcac ggtatccgaa taccacctgt gcggagtata attctgtcgg ttccggaaat
    11521 tccctggact tcacctatgg ggactgtgtg cagaagatcg aggtaaaaac tgcggatctg
    11581 acatttcagc cgaaggggct ggaggaaatg acagtgggtg aaacacagac gctggaactg
    11641 gaaatgtcct atcagcagaa aaagggattc cttgcggaag aaccgatttc ggcaagctgg
    11701 cgctcttccg attcctctgt tttgacggtc agcggcaata ccgtgacggc agtatccgca
    11761 ggcactgcat ctgttatcgc atcggtaaac ggtacagatt atgccgcaga agtgaccgtg
    11821 aaagaggcat ctcagacgac aacacgcccg gaactgacgg atgacgagct ttacctgtca
    11881 tttcacaatg tagaattgtc actggaagag ctggaaaagg caaattatat tgtcaatatt
    11941 ccggtgtatg cctccagaga ttttaacaag attcagttca gcgtgaacgc agatgagcgg
    12001 ctggtatttg acggcgcaag atttggcgca ctgtcgagtg tacacagcag tctgattggt
    12061 tcccgtacca cgtgtgtggt acggggaacc gccggaggtg acattgcggc aggaaacgca
    12121 ggcgttgtcg cttatcgcct gccggaagat gcacagccgg gcgatgtcta tacgctggag
    12181 accacagcag aagagacgat gcagtggtca gatgccagcg gcaattccgg cggcatccag
    12241 tgcagcggca atgtttccat caccattacc ggcggcagca cctcttctgc aacagtgact
    12301 accgctacct ctgcgccgga gacttccacc acagtcacta ccacgaccgc agaggcgcag
    12361 aaactccacg tggcactgct ggatgtacag atcagccggg aggatctgga gaagaaccag
    12421 taccgggtca agctgcccct gtatgtgccg gattcgttct cctcgctccg gttcgccgtc
    12481 caaaccagca gccagctgaa acaggtgggc tgtgagatca aggactgtca cgtaatgtct
    12541 gcgtccagtg acggctattc cagctattcc gtttccagtc tgaccggcga ggatgttccc
    12601 ggcggcgaga tcggctggat caccttccag ctgccggaga gtgcggctgc cggggatacc
    12661 tattatgccg atgtgctggc aggcgcatcc agcggctgga accgttctga cggcacttcc
    12721 ggcggcatta ctaccggcaa cggttctatc cagatcgtag accacaccac cacacagacc
    12781 acggaaacta catctgtaac aacgtccaca gagaccacaa cctctgccgc accgggaaag
    12841 aatctgaccg tttccgtggg aaatgttacc gtgaccccgg aagaattggc ggcagaagat
    12901 aacatcgtag agctgcccat ttccgcagac cggtcgtatt ctaccatcgc tttcggcatc
    12961 acctgggaca gccgtctgac ctatgtggag agcggcgcag ataagggcat gggcattgcc
    13021 ggacagggtg accgctttat ctggtgggcg aacagcgccg gcggaaatgt gcctgccgga
    13081 aagatcggct atgtccggtt ccgtctgccg gaggatgcag aggacgggga tgtctatgtg
    13141 gtgaacctgt ctgcacgctc tgccggcggc aaggatgccc gctggacgga caatctcacc
    13201 gggctgtcgg gcgtgccgct gacggtgggc ggttcgatcc gggtcggtgc atctgggcag
    13261 accaccacag agacgacagt ctcctcgggg accacaacca ccacagcggc agtacagagc
    13321 atcctctacg gagatgtgaa tgtagacggc agagtggata tcacagacgc tgtactgctg
    13381 aataaggcgg tttccggtgc agtatcgttc gacagccagc agcggagcaa cggagactgc
    13441 gatgtcagcg gcgagatcaa cggtacggac gccattctgc tgatgcagtt cctggcgcat
    13501 atcattgata cgctgccgga tcggggataa ggcgtaatac aaacagaacg agcggttctt
    13561 cccggagcgg aagagccgtt ttttgtatta tcttgacttt tctaaagaga tatggtaaga
    13621 tatatatgtt atctttgtgg taaaaatatt ttgaaaaaat gagaggaaga tctgacatga
    13681 aacaaaacac gatctggaca ggcgtccttg ccgtggcatc ggcgtttgcg ctgctgctga
    13741 ccggatgcgg aaagtcacag actgccgccc gtgacacctc ggcgaaacag aacgagtcgt
    13801 cttcggcggc tgaaacctct gcggcggaag agacaagccc ggcggtggag caggcagact
    13861 ttcagctgaa accgtatgcc atggacggcg tgggctgtgc ggtggaagtg ccggacagct
    13921 ttacggcgga cagcagcagg gcggcgatct ccggacacct ttcctttaat gggacggaga
    13981 ccgatgactg cattgtggca gctgtctatg atgccaagcc ggaagagacg ttcgatgcgt
    14041 tccaggagaa cgacctgttt gaactgctga ccttttcgac cacactgccc caggtgcagt
    14101 atttccggga aacggagatc cccaatgcgg acggtgtttc ctgcaaggcg tatgtgggag
    14161 agacgctggg gacaattgcc gggaaagatg cgtatgtgac catcctggcg gcaaactgcc
    14221 cgaaggagaa caagctgttc gtcttttcca tgcgggacat gaccggtgaa tttcagaact
    14281 accgggacaa gttggcagac cacatctatc tgaaccagaa cggctatgtg gtggaaaacg
    14341 accagctgac agatgcgccg gaaccggagc cgctgtccta ttccaatgat gccatcggct
    14401 atcagatcag cctgccggag ggctgggagc ggctgacgga taccagcagt tcctacgggc
    14461 tggagacgat aacctccagt ttcgacaact acgacctgtt cttgaaccgt gacatggact
    14521 atgcgctcat tgcagcaaag tctatgagcg tttcggatac ggaattctat gccgccataa
    14581 aacagaacca gtctgcatgg gtcggggcag gcggaaatga actggtaacc tccaaggaat
    14641 gcaccgtgct gggctatgcg gcatatcaaa tggtcatcaa ccacaacgcc ggcgctgaca
    14701 aaacttccag cgtggcatgg ttcatcaaca agaccggcga caatcccgaa atgctggcga
    14761 tccgctattg ctaccaggag gacagtgcgg cagaatttgc agatacgctg ataaacagca
    14821 ttacattgca gtaacgctgc ggtcagaaag aacgacaaaa gcggttttcc ccatgcaggg
    14881 aaagccgctt tttggtggtt ggactgccgt ggcgtctgcg caaactatga caaagttcct
    14941 taaaaagtta caaaatagat ttcaaaattt gccggaaaag ggtgcggatt tcgtgagata
    15001 gtcccaggat tccacagaag attttgtgca gatgtgcaaa agttccggaa aatccggtcg
    15061 gaaagtcttg caaaacggtt acaaacgtgg tataatagca attgtacggt gaccaccgcc
    15121 ccgtggacaa gcactcgctg gtccgtgggg aattcacgtc tgccgtccgc ttttgtccgg
    15181 tacacacgga cagggcgggt gcaatgcagg cggcacacaa gtttcaagag atggcacatc
    15241 tcgggcggat tgtccggact gcgacagatc ctctgatccg tcaggacaat gtcggcagag
    15301 cgcagtacac ctttccgcct tcggaccgga caggcgtgtg ccgcaaactg tgtggaatgg
    15361 gaaatgatac ttcccgtatt cctgcggtgc attgcagcgg cagttccttg ccgtactgcg
    15421 atgcagcgac tgctgtcagc gcaaacacca caaaggagcg tttttatgcg taaaaaaatc
    15481 atggcagtcg ttttatcctg tcttactatt attggtttgg tcgtttcgtc ctttgccgta
    15541 tcgttttcgg catctgcggt ctccgtggac gaaatgacaa aagccgctgt acagatcatc
    15601 tcccggagtg agggtactta tggtacaatc aaccggaatg acaacggtgc ggtcagcatc
    15661 ggtatgttac agtggcacgc cgaccgggcg cttcagctga tgcgttctat cgccaatgcg
    15721 gacaccggtt cggcacagtc gattctgggc agcaccttct ataacgaagt gatgactgcc
    15781 tccagctgga attcccggac gttctctgcg gcagagggta cggcagcgtc aaacctgctg
    15841 accactgcgg caggcaagtc caagcaggat gcgctggcgt acagcgatgt gcagggttat
    15901 atcagcgctg gtcagaatct gggcatttcc aatgccggcg ttctggtcta ctatgcagag
    15961 ctgtataacc ggggcatggg cgtagcgaga cgcattctga atgcggctgc aaacggaggc
    16021 gcttacagca gtgtaacgct gtccaagctg cacagcacgg cgcttgccaa cagttccagc
    16081 tattacacca gccgtctgaa caatgcgtac aacaccattg tttctctggg ctggggcgat
    16141 gctgcggcaa cgcccggcgg cgacaacggc agcagcggtg cagatacctc tgacttctca
    16201 gagagctatg ccggtacata tacggtaact gcatcttcgc tgaacgtgcg cagcacaccg
    16261 tctacttccg gtgcaaaggt cggcacactg ccaagcggtg caaaggtgac cgtatcctct
    16321 gccaacggca gctgggcagc ggtttcctac aacggtatca gcggctactg ctctatggat
    16381 tacctcaagc aggaggctgc acagaccaca gcgccgccgc agacgactgc accggcagag
    16441 accacaacta ctgcggcacc ttcggatgcc aatttcagcg aaagctatgc tggtacctac
    16501 agcgtgactg catcttctct gaacatacgc agtgcgccgt ctacgtctgc ctctaaggtg
    16561 ggcagcattc ccaacggtgc aaaggtaaca gtcactgccg cagacggcaa gtgggcagcg
    16621 gtttcctata acggcgtcag cggttactgc tccatggatt acctcaagca ggagagtgcg
    16681 cagaccactg caactacaac ggctgcaccg gaagagacaa ctacaaccac aacgactaca
    16741 actacaactg ccgcaccgga tacaacggca ccggcagaga ccaccgcagc accgacaact
    16801 accactgaca ttgacatggg tcgtgtggac gagagctatg tttcgctcta cggcgatgtc
    16861 aactgcgatg gcacgatcga tattctggat gccgttctgc tgcaaaagta tctgaacggc
    16921 agcgtacagc tgaactacac gcagctcgcc aatgcggact gccagaagga cagcgaactg
    16981 gacaacacgg atgtaactgt gattatgcag tatctgctga gaagtttcac ttctctgccg
    17041 gttgcataac acgacaaaac aataagacag ataaaacgac aaacgctgta cccggagtga
    17101 tctgggtaca gcgtttttgt gttatgtgac gtgttattgc tgatgatgca gggaagtgac
    17161 aagattcgaa cttgcgggat ttggggtgga tttgacgcaa tttcgggctt ttttggggga
    17221 ggttgccatt tttggttgcc attgaaaatg tagaggggaa ttgataaagc tgcaaattca
    17281 ggtttaatac ttcacctttc cctcagccac tgctctgaga tgtgcaccgt agggactctt
    17341 tccataagcc tcagcggatt ggagcaggtc atttttgctg atccagccgt tgatgaatgc
    17401 aatttcttct ggggctgaaa tggtgattcc ttggcggttt tgtatcattt ctacaaagtt
    17461 tgctgcatca agcaggcttg aaacggttcc agtgtcaagc catgcaaaac ctcttccaag
    17521 cagctgcaca tcaagcagac cgtcgttgag gtacatatcg ttcagtgtgg tgatctccag
    17581 ttctccacga gccgaaggtt taacagtatt tgctctttcg ctgacgcctg ccgggtagaa
    17641 gtaaagacct gtaactgcat aattgctctt gggatttgat ggcttttctt caatgctgag
    17701 cgctttgttg ttttcatcaa attcaatgac gccaaatcgt tccggatcgg ggacataata
    17761 accaaatacc gttgcacggc cgttctcttc tgcatttttt acagcagatt tgagcagtgt
    17821 tcggaatccg tttccataga aaatgttgtc gcccagaacc attgcgccag cctctccgtt
    17881 gagaaatttc tcgccgagaa tgaaagcctg tgccagtcca tctggactgg gctggacttt
    17941 ataggacaga ttgatcccat actgactgcc gtcaccgaga agtgcctgaa accgtggtgt
    18001 atcgtcaggt gtgctgataa tcagaatatc ctggatacct gccagcatca gtgtggacag
    18061 gggatagtag atcattggtt tatcatagat cgggagcagt tgtttcgagg tgacttttgt
    18121 cagcggataa agccgtgtgc cgctgccgcc tgcaagaaca attcctttca tatccgatta
    18181 ctccttttca aaactgaaat gtatccttta atccaaccca cttctgatcc ttatcactca
    18241 gattcagctt cgtgccatct ttcagtgcat nnnnnnnnnn ctcagcagag gcggagccat
    18301 tataatttcc aaccaattct ggccaaacga tcccgatctc cgggtcattc caagccagac
    18361 caccttcgtc attcggatgc cagaagtcat cgcacttgta gcagaattcc gcaatatccg
    18421 aaagtaccag aaagccgtgg gcgaatccct tggggatcag gaactgcttt ttgttttctt
    18481 ctgtcagttc cacaccatac cacttgccgt aggtgtcaga atcctttctc aggtcaacag
    18541 caacgtcaaa aacactgccc ttgatgactc tgacaagctt tgtctgcgga aactgtttct
    18601 ggaagtgcag tcctctcagt acgcccttgg tggagcagga ctgattgtcc tgtacaaagt
    18661 caatatggat cccagcctct tccatgtctc tctgggagta agtctccatg aagtatccac
    18721 gattatcgcc gtgaacggta ggggtgatga cgcaaagccc ttcaatgccg cctacattct
    18781 tttctactgt aatctgtccc attagtctgt aatctccttc agatatcttt ccagtgcatt
    18841 cttccaatcc ggcaggggag tgaaaccgtt ttcaatcagt ttcgatttgt ccagtctgct
    18901 gttaaagggt cttgctgcct tggaaagacc atattctgct gttgtgacag gcaccacctt
    18961 ggtggaatac ccagcctgcc ggaaaatctc acaggtgaag tcataccagc tgatatagcc
    19021 gccttcgttg gtggcatggt aatagccata cttctcggtc tccgccatat ccaccagcag
    19081 ccgtgccagg tcgtaggtat acgtcggtgt gccgatctgg tcattgacca ctcgcacggt
    19141 gtcgtacttt ttgcccacgt tcagcatggt tttgataaag ttcttgccgt tcttgccgaa
    19201 tacccaggca atgcggacaa tgaaatactt gtccagtgta ctgcttactg cgagttcacc
    19261 gtccagcttt gtctgtccgt agacattctg cggtgcgtag tccttgcagt ccggctgcca
    19321 tggttctgta ccttgtccat cgaaaacata gtcggtggaa atgtagatca tcttgcagtt
    19381 cagctttttg cagacatttg caatattctg agttccgtcc acattgatgg cacgtacctt
    19441 ggggagattc tcctcgtcct cggcagcatc tactgcggtc catgcggcac agtggatgac
    19501 aacgtctggg ttcgcttctg tgatcgctgt ttcaacggcg tctgcgttgg tgatatccag
    19561 cttgacatag gcacagtccg cagtaacttc atccagaata tcgctgccga ttgcctcgta
    19621 gccacgcttt ttcagttcgt tgaccacatc aaaaccaagc tgtccgccga cacctgttac
    19681 aaatactttc atctgtatcc ctcaccgatt gctgtacatt ttttcatagt agtcctgata
    19741 ctcgccggaa ataatgtcct tccaccattt ctgattgtcc agataccact gaatggtctt
    19801 tttgataccg tctgcaaact ttgtttccgg cagccagccc agttcgttgt ggatcttggt
    19861 cgggtcgatg gcataacgca tatcgtggcc cttacggtcg gtgacatagg taatcagact
    19921 ctccggcttg cccagttcct tgcagatcag cttgacaatg tcgatgttct tcatctcgtt
    19981 gtggccgcct acgttgtaga cttcgccgac cttaccattg tgaatgatga ggtcgattgc
    20041 cttgcagtga tcctctacat acagccagtc acgcacattt tcgcccttgc cgtaaaccgg
    20101 cagcggcttg tcagccaggg cattggcgat catcagcgga atcagctttt ccgggaagtg
    20161 atacgggccg tagttgttgg agcatctgga aacggttacc gggagaccat aagttctgtg
    20221 ataagccagt accagcagat ctgcacctgc tttagaagag gagtaggggc tgctggtgtg
    20281 gatgggggtc tcttcggtaa agaacaggtc cggtctgtcc aacggcagat cgccgtagac
    20341 ttcgtcggta gaaacctggt gatagcgctg aatgccatac ttccggcaag cgtccatcat
    20401 aaccgctgtg ccgaggatgt tggtctggag gaagatctcc gggttctcga tagagcggtc
    20461 tacatgagac tctgccgcaa agttgacaac gatgtcgggc ttttcctcct cgaagagctt
    20521 gtacacgccc tcacggtcgc agatgtccag tttgacaaac cggaagttcg gattgtccat
    20581 caccggttcc agtgtggaca gattgccggc ataggtcagc ttgtccagac aaacgatgcg
    20641 gtagtctggg tgtgctttta gcatatgaaa tacaaaattt gagccgataa agccggcccc
    20701 gcctgttacg atgatggtca tgatagatac tccttagaat gaagtgtgtt atttttatta
    20761 tatcatactt ttgggggaat tgcaataaaa aatgtaccgc tggtgggcgg tacattgggg
    20821 gaatatgggg ataggcaata tctgaaatgg tatcaataat ttaggagagt tctttctttg
    20881 ctgcggcgag ggcgacttct atggttttat ccatgtcata gtatttgtat tcgccgaggc
    20941 ggccaccgaa aatgacattg tcttctgctt ctgcaagggc tttgtactgt tcatacagtg
    21001 tgctgttttt agcgtcgttg acggggtagt agggttcatc tccaagtttc cattccgaag
    21061 aatactctct cgaaataact gttttcggca gatcgtttcc gttttcatct ttgccgaact
    21121 cgaaccattt atgttcaatg attcttgtcc agggagtttc tctgtctgtg tagtttacag
    21181 ctgcgttacc ttggaagttt ggcttatcaa gaatctcatt ctcaaatctg acagagcgat
    21241 actccaaagt acccagctta taatcgaaat acgcatcgat cggaccggtg tagattacct
    21301 tttcagcgag cttatccagt tcttctttgt tttccagata gtcaacattc agccgaactt
    21361 caataccttc cagcattttc tcgatcagct tggtatatcc accgatggga atgccttgat
    21421 agagtgcgtt gaaatagttg ttatcgaatg tcaggcgaac tgggagccgc ttaatgatga
    21481 atgcaggcag atccttgcag tctcgtcccc actgcttctc tgtataacct tttacaagct
    21541 tttcgtagat gtctcgtcca acaagcgaga ttgcctgttc ttccagattt ttcggctcac
    21601 cagtgatttc ttttttctgc tcttcaattt tggctgcggc ttcttcggga gtcacaacgc
    21661 cccacatttt attaaatgtg tacatgttga atggcatgga atagagctct cccttgtaat
    21721 tggccaccgg agagtttgtg aatcggttga agtcaacaaa gcttgtgaca tagtcccata
    21781 cctctttgtt attggtatgg aaaatgtgtg caccgtactt gtgtacattg atgccctcga
    21841 ccttttctgt atagatattt ccgcctacat tggggcgctt atctattaca agcacgcttt
    21901 ttccggcttt ttttgcctgc tgggcaattg ttgctccgta taagccggag ccgacgataa
    21961 ggtagttgta gttcatttta tttttctcct tttaacttta ttataaagtc attcttgttt
    22021 caatcatact ttttattaat cttctatatg cttcttcaag attcacactc ggtttccatc
    22081 ctaattttct gagcttatct gtatttaatt tcatttttgt atcacatgca tagccatagc
    22141 tattagtttc tggaatatca aaaataacat ttatttgtcc gcttgcgagt tttgatgcaa
    22201 ccatttttgc catatcagct attgttgtat ggcattcttc atttgaaaca ttatacgcat
    22261 tttgattttc accattaagc attattgtaa gaattgcagt tatagtatcc cttgtatagc
    22321 aatagtttcc ttccgaaaga cctttagtat gaagtactat atctttattg taaataacac
    22381 ttcttgcaaa ttgtgcaaac accctatttt cacctggaag aatcccagct ccaaatgttt
    22441 gtgaaagtct tgcaattcgt actggaactc tatattgttc actataagca atacacatat
    22501 tttcacacat tcttttgccg agagggtagt tgcttctcaa agccagggga ttaatatatc
    22561 ccattgtatc ttcatgtaca tatgtttcat cattaaaaga accataaact tccattgaag
    22621 aaatgtacac aaatgcgttt acagcgtgtt tttttgcaat ttcaagtatg ttgtgcgttc
    22681 ccattaatgc agtcataatt gtatctacag gttgggaaat catttgtttt gaagcggtaa
    22741 tgcttgcagc atgtataata taatcaatag agatgttaga gggtatgttg ttttcagtta
    22801 tatctgtaat gtcactttga ataagaataa tgttatcctt tttatctatc ataccaaaca
    22861 ttttttcaaa ttttgatata ttcctta
//
LOCUS       DAWENO010000014        22615 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_952_length_22615_cov_13.544504, whole genome shotgun sequence.
ACCESSION   DAWENO010000014 DAWENO010000000
VERSION     DAWENO010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 22615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22615
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_22615_cov_13.544504"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4776 a         6094 c         5720 g         6025 t
ORIGIN      
        1 gtgcattgcc caggaatgca gcgccgatga tgccggcatc gttgcccagc tgggcgatca
       61 cgatctcggt attctttgcg gagtcacggg tgtagacttc cttcttgaca atttccttca
      121 tgggaaccag cagcggatcg ccctcgttgc agacgccgcc gccgatgcac aggatatccg
      181 gctggaagat gttgatggtg ttggtgatgc cggctgccag gtacttgatg tagtcgtcta
      241 ctacctgctt tgcagttgca tcgccggcac gcatatagtt gaatgcagtc cgtgcggaaa
      301 tgcgctcttc gtccttcatg gcgctgtccg gatttgctgc cagtgcctcc ttggtcatgc
      361 gcaccagacc ggtagcggaa gagtatacct caaagcagcc ctttctgccg caggtacact
      421 gcggaccgtc tacagagatc acggtgtgac ccacctctgc gccgccgaag ttggagccgc
      481 tgtagatctt gccgtcgatc acaataccag cgcctacgcc ggtacccagg gtgatgcaga
      541 ctgcattctt tgcgcccttt gctgcgccgg caacatattc accgtatgca gcagcgttgg
      601 cgtcgttttc gataaaggtg ggcttgccca gggcagtttc caggtacttt accatggggg
      661 tgttgtggaa tcccaggttg ttggaatatt cgatcacgcc ggtggcgctg tttgcaacgc
      721 ccggtgtgcc gacgccgatc cactcgatgt cagccatggt cagaccagcc tgttctgctg
      781 cctccttgga aaccttcgcc atatctgcgg cgatctcctc ttccggacgg ggcaggtttg
      841 tcttgacgct tgcctttgcc aggatctcat agctctcgtt gaccacgccg gcaacaatgt
      901 ttgtgccgcc caggtcgata ccaatgtaat acttcatttc gtttcctcct aaatattcaa
      961 atccattgta tggaattgtt aacctaactt ttcttccacg ccggtgacga tataccggaa
     1021 tccgctgccc tttacgctgt atgcgcccag accggcaggc agcaagacgc tgtcaccctt
     1081 tttcatggga tagctgccgg atgctgtttc cagcaccgcc tcgccgtcca acgccagcag
     1141 gtggcggaac gtctctgtcc ccacggtgaa atgcactttg ccggaaacct ctaccacgtc
     1201 cgagataaag tacttgcagg actccagtcg gcggacactg ccgccgtcaa ccggctgtgc
     1261 cggatactga gcggtcggtg cgatggggct gaggttcgtc acatccagcg ccttttcgat
     1321 gtgcagcgga cgcagcttgc cgtctgcgcc acgtctgcca tagtcataga cccgataggt
     1381 ggtgttggag ttctgctgga tctccgccag caggatgcct gcgccgatgg cgtggagcgt
     1441 gccggacggg ataaagtaca gctcgcccgg cttgacctct acccggttca aaatgtcggt
     1501 cagggtctgc tcctgaatgc gctttgcaaa ctcctctttg gagaccgcac ggttcagccc
     1561 gtaatacagc gcagcgcccg gtgcagcatc catgatatac cacatctctg tcttgcccgg
     1621 ctcgccttct acccgcatgg catagtcgtc atccggatgc acctggatgg acaggtcctg
     1681 cttggcgtcg atcagcttaa tcagtaccgg aacagtggtg cagccggcgc agtttgtgcc
     1741 gagaaaacca gtgccgtgtt cgtccaggta ttcccggagg tcctttccgg catctgcccc
     1801 ggtggtcaga atgctgctgc cgtccttatg gcaggcaagc tcccagcttt cggcgatctt
     1861 gtcggcatcg cttttcttgc cgtattcttc ccggagtcgg tttccgcccc agagatagtc
     1921 cttgcagatc ccctgcaaaa aaatcggttc tgtcatagtg gttcctttct aaaattcctg
     1981 cagtccgaac tgtttccgga attcgtccat gctgccggcg tagacgttca gatcaatatg
     2041 ggtctgatag ccgtcatagc cctgcagttc cccgtcgtcc gtatactgcc agaatgtcca
     2101 gtcgaaatag ggtttataat agtagttgct gatccagatg ggatactcgt ccgcaaagct
     2161 tttgatatag gtgcggtagg tgttgggggc tgcatagaga atgggtttta ccccgtagtg
     2221 ttcttccagc agctccagca tttcccggat attctccttt gcagtatccg tatccggcat
     2281 cacgccgccc agggactcat agagttccac gtcgatcacc ggcggcagcg tgttttccgt
     2341 caccggaacg gtctggatga agttttccgc ctgcgccctg ccgctgtcct cgaaccggaa
     2401 aaagtggtat gcacccacat agatctgcgc cgactgcgcc gtttcccagt tctgggcgaa
     2461 ctggctgtcg ttgtggtcga tgccctcggt ggctttgata aagcagaagt ccacgccctg
     2521 ggatgccagc acgtcccagt cgatgttccc ctggtagtag gacagatcga ctcctcgcac
     2581 cggataatcc tgcttgctgg gtacatctgc cgcctgccgt ccggtactgg tgagatagat
     2641 gtgcagcagc cgtctgccgc ccacaaaggc gaacaccagc cccgcaagca ccagcagggt
     2701 catgatccag cggaaaatgt attttccgta atgttttctt ctcacgaggc aatatcaccc
     2761 attacaatgc cacgtccatc ggtgcagaaa taaactctgc cgtagacatt gctgtccccg
     2821 gtgatgtact tggggttcag attgccgaac ttgtgctggt catcgttgat ccggagccaa
     2881 gtcttgccct tgtcctcgga tttgtagatg ccatagccgt tttcgccgtc atcgccctct
     2941 gcgtaaacta ctggatagct gttgtccttt ttaccttttc cgaaaccaat gctgtctacc
     3001 gtgggcaggg tctttacggt ctggaagctt tcgccaccgt cctcggtgta cataatcata
     3061 gtgctgctgg agatccagac gttgccctcc tgcccgaaca cggtcaccgg ctctgcggca
     3121 tctgccacca tctgcccggt ggactcgaag gtcacgccgc cgtccttgga cacgtagaac
     3181 agtccgtcac aggtggcgta gaacaccttg gggttcaccc ggtctgcggc aatcttggcg
     3241 ttaaagccca gaccctctac ataataccag gtctcgccca ggtcagtggt cacatagggc
     3301 tttccggaga cgctgttcgg cacccagatg gtggagctgc cgtctgcgga cattgccacc
     3361 tgtcccccgg tcgccgggtc gggcttatgc tttacggtct cccaggttgc gccgccgtct
     3421 gtggtgaaat tgatggtggt ggtcttttcc tcgctgaggt ataccgccgt gttggggttc
     3481 tggaacgcca catctaaatc tgtgggcttg ccgttcttca tgaagtgggc atcgtccggg
     3541 catttcgtca cgtcctcatg ggcgaaaccg gtcagatctc ccatgatgga atacagctgg
     3601 ggcgaattgt ccttgctggg cggtgccagc atcttgaaca ctgccgtttc ttccagcccg
     3661 taagcatcaa aggcaatggt caccggctct gtgccgatct tggtcatatt ctccgtggaa
     3721 tagatggtcg caccggtgcc gtacatcact tcatccgaat tgaacgggtt gatgttcaca
     3781 tctgccaccc accagccggt tttcgcctgg ttctcgcctc tgccccagtc cagccacgcc
     3841 gcccgggagg tgtccatctg gtagttgttt tccttggtgt ttaagttgaa cagaccgctc
     3901 caggtcttgc cgccgtctgt ggtatagtac aggttgtcac cgttgtccga ccaccagccc
     3961 agggtggaga ccaccacggc atccgggttc tgggcatcta ccgagatgcc gccgaatcca
     4021 ccctgtctac cgtcgcccag gtcaggcgtg atgtccgtga aactgtctgc cttggtatcg
     4081 tacacacaga ctgcgccggc agtgggagac aggttcggac cgcagttgtc ggaatatgcc
     4141 agatacagct tgccgtccgc agaaaacgat gcgtgcaggg gatacattcc cggttcgttt
     4201 gccgccagcg ccgtccagct ttcgccgccg tctgtacttt tatagataca ggtgccgtct
     4261 gtcatggctg cacccacgta catatctgtc gaagtcgggt caaatgtcac ccagagaatg
     4321 ccgatactgt tgctctcctg cttatagttg ccctttaccg ggaacgaagt gacctcttcc
     4381 cagttcttgc cgtagtcaga ggttttccac agaccactgt cacggctgcc gaaccagacc
     4441 tccttgttgt cgttgggatt gacgcacatc cgctcgccgc agccacgtcc ggactggttg
     4501 ccgccgcagc tgaacggcgc atcaaagcgt gtccaggtct cgccgtagtc gtccgaggca
     4561 aacagcgccg catcgtcact catataagta ccgccggcaa aatacacccg gttcggttct
     4621 accgggtcgg tcgccatgct ttcgatgccg atatagcccc actcgtctgc gcccagaaaa
     4681 tcggtgagcg gcacccagtc ggtgtccttt ttccggcggt acagtccgcc gatatcggta
     4741 cgggcataga ccagaccctc ttccttgggg ttatactcca tgccggtgac ataaccgccg
     4801 ccgccgatgg caacattgct ccaggtataa gtttgggcgc tgcccgaagt acagccggtg
     4861 ccgatgccgc agagcatggc gccgctcagc aatactgccg taagccgtcc tcgctttttc
     4921 atgtgtgtgt cctccgtatt ccattgcagg gagactgcaa aaatttccta ctttctataa
     4981 taaccgaaaa cggcggatta gtcaatatct cttttcaatt tttaaggtgc aaattaaaaa
     5041 acgattatta tctaaattga atgaaattcg acaaaaaatc atattgacaa atgtttttcc
     5101 ttgttttata ataaaaatat acaatttaac catattatta aacacagaaa cgcaattacg
     5161 tgtcctcatg gcatgaatag agttgcagca aagccatcct attcagagtt tactttaata
     5221 tacaaaaaat attcacctta ttacatctga ccatcgccga aatcctgtat cataatacta
     5281 tgttttcaaa ggaggttatc tatatgaaaa agaaacattt tgctgtttta acttttgccc
     5341 tttgtttttc ccttctttca actgcttgtg gaacatcgaa aaacagcact gaatccacgc
     5401 agacacaaaa gggacttgtc cttacagtct ccagcacagc cgattttgtc gatcaagcca
     5461 caactttaca agagctaatc gactcttcag acattgttgc aaaagttaac gtccaaaaag
     5521 ttgattcctg tgtttacaaa ggcactgatt ctgttgttac ttctattacc ccagaaattt
     5581 tggagatata taaaggcacc tataatggcg agactttcga tatgtacggt gggtatatta
     5641 gctacgcaga gtatcaaaac gcacccattt ttcaagagaa cgatgacgct ctgcctttcg
     5701 atacatcaag ttattctgaa tctgaaatca ataacgcaca actttactgg gactggtgca
     5761 acgtatatat tccaagcgcc ggagacacac ttctttactt cggcaaagta agtgacaaca
     5821 acaacttttt ccttacgaat gcttatcaag gactatttca tttagaagat gggaactgga
     5881 caaatcaggc tttaatcact ggttcacaaa cgcagcaaga actgctcgtc acagacctgt
     5941 tgaacatttc cggttcgtct gctgtggaat cccctcaaac cggaatatca aaaacaattg
     6001 atgcaattac gtacacctat cccggcagcg attacgatac tatgatttcc attccagaaa
     6061 gcgcactcac agaagcaata caaaaaaata tttctgatgc agaataacag caagagcggc
     6121 aatgcagcga cattgtcgct ctgtttattt atccccacac caccggcagc ggcgtatctg
     6181 catggtcacc gatgtgcttt tccatccaga ccatatgata ccaccgcccg aacttatagc
     6241 cgcactggtg gaattcgccc accatccggt agcccagctt ttcatggaac cggatgctgt
     6301 cccgtgtcag ataggggtcg gtttccccca tagggcaggc gatgcaggcg ttcatattga
     6361 ggaaatgccg ccgccgcagt tcctgttcca gggcatcgtg gagcagtctg ccgatgccac
     6421 ggcgtttctg gtgctgttcc acatagatgg aagtctccac cgaccagtca taggcagcac
     6481 ggtcgtgaaa ttttccggcg taggcatagc ccaccggcac gccgtcctgc tctgccacca
     6541 gataggggta tttttttaag gtatccgcaa tgcggcaggc aaattccgtc tcggtgggaa
     6601 cgtcgtattc aaaggtgatg gcagtctgtg ccacataggg cgcataaata gcaagcagtg
     6661 ccagggcatc tgccgggact gccgggcgta tctgtatgga catggtgatt cctcctttgg
     6721 tgattctctg tctagggtac accttttgag atggattgtc aagacttttg gcgcaaaaca
     6781 gcccccagcg gtgcaaattc cgctgagggc tgtttcatta tgtgaaatcg attatacttt
     6841 caaagaaatg gggttgcctt agttcttcat ctcgaagtgt tcgcccagga tatccttgtt
     6901 tgcgtccaga atcggctgga tgcactctgc cacgaattcc tcggtctgct gttcgctgcg
     6961 gccggtgaaa ttctccggtt tcagaatctt gtccaggtct tccttggtaa tcatgaacgc
     7021 cgggtctgcc aggagcatat cgcacaggtc gttgtccaga ccctcttcct tgacgtgctt
     7081 gccggcaacc atggaatagg tacggatctt ctcgtggagt tcctggcggt tgccgccttt
     7141 ttccactgcg tccatcatga tgttttcggt tgccataaac ggcagttcac gcatgatgcg
     7201 ctgacgaatc atcttcggat aaaccaccag accgtcggtg acgttcagca tgatgttcag
     7261 aatcgcatcc actgccagga atgcctccgg aacagaaaca cgcttgtttg cggagtcatc
     7321 cagagtacgt tcaaaccact gggtacctgc ggtgaatgcc gggttcagac tgtcgatcat
     7381 cacgtagcgt gccagagaag tgatacgctc acagcgcatg gggttgcgct tgtatgccat
     7441 tgcagaagag ccgatctggg tcttttcaaa cggctcttcc atctccttga agctctggag
     7501 cagacgcata tcgttggaga acttgctgca ggactgtgca atgccgctga gggttgccag
     7561 aatctgagag tcgatctttc tggaataggt ctgaccggaa accggaacga caccgtcaaa
     7621 gcccatttcc ttggcgatct tcgtctccag ctccttgacc ttatcggtat cacccttgaa
     7681 cagctccata aaggatgcct gtgtgccggt agtacccttg gaacccagca gcttcagatt
     7741 tgcaatacgg tattccagtt cgttgaagtc catgagcagc tcgttcatcc acagggtagc
     7801 acgcttgccc actgtggtca gctgtgccgg ctggaggtgg gtatatgcca gcgccggcat
     7861 tgccttgtat tccagggcaa aacgggacag gttcgccaga acgttaatca tcttccggcg
     7921 cacgatctcc agcgcatcac gcatgatgat gatgtcggtg ttgtcgccca catagcagga
     7981 ggttgcaccc aagtggatga tgccggcagc cttcgggcat acctggccgt aagcgtacac
     8041 gtgggacatg acatcgtggc ggcggcgctt ttcctctgcc tcggcaacgt catagtcgat
     8101 gttgtcgatg tttgcctcca gttcgtccac ctgctcctgt gtcaccggca gacccagctc
     8161 catctctgca cgagccagag ccacccagag acggcgccag gtggaaaact tcttgtctgc
     8221 ggagaaaacg tgctgcatct cttcgctggc atagcgggtg cagaacggac tttcataact
     8281 tgtggtatta cgcatatttt tgtacctctt tcttttggaa tccgttgacg gaatcgtttc
     8341 ttgttctatt atagcatatt tcggcaggat tggcaagagg gcgaaaaaac cgccccgaca
     8401 ggaatgccct gccgagacgg tctttcacga tgctggagac aactcacacg gaaaatgtgt
     8461 gccttatccg gtcgttacgt tttacttttt tctgcgcttt ttgcctgtca cgagcgctgc
     8521 agccagtgca gcgcctgccg ggatcagaag tgccggaagt gcctcgccgg tctttggcgt
     8581 tgaggagact cttgtgctgc cggaggacga accggaactg ctgccgctgc tgttgctgcg
     8641 ggtagtggtc gtcgtctggc tgctgccggc agtggtggat gcagaatcac tgctgtccgt
     8701 gccggtgccg tcggttgtca ccagggttgc cgttgtggtc ggcgtatgct ctccatttcc
     8761 ggcggtggtg tttctctcgg tcgtggtctc ttcggttgta gttgtggttg tcgttgttgt
     8821 agtcgttgtg gtggtagttc cttcggtttc agaagtggtc gtctctgtgc cgatgtactc
     8881 atccaccatg acgattgcct ttgtgtctgc actatatgcg gtgcttacga tctgccatgt
     8941 accgtcctcc tggacgacag gtgcgccgtt ctcatcgacg gcaactgtca gcagcagcag
     9001 atcgccgttt tcatccagct ggaagtagat cggctctgca attgcatagt ctgccggagc
     9061 gaggacttcg gtcaggcagt acggtacgcc tgtttcgatg ttggcaaaga cgtgagacac
     9121 gccctcttcc gatacccact ggtcgatgac attgcccgac tggtctgtga tcgtcagcgt
     9181 tgcgccgccg atctccttgc cgcctgcgtc gcacttgcgg atgagatagc cgtccggacg
     9241 ttctgtggta acggtggtgg tttctgttgt ttctgtggtc gtttcttcgg ttgtggtctc
     9301 ttcggtagtc gtatcttctg tttcagaggt tgttgtgatt actgtggttg tggtcgttgt
     9361 tgctgcggtc gtagtagtac ttgtttccgt tccggtctcc gatacggtgg tcgtctcggt
     9421 cggtgctgtg gtatccgtct ccgtttccgt tgcagaaaca gtcgtggtat cggcgctgac
     9481 agatgttgtc gtctcagaac cggtttccga agtgctttcc gacgatgtgc ttaccgcaga
     9541 ggtagttgtt ccgctggtgg tggtattggt cggacctgca ctggattctg tagaacctgt
     9601 ggttgatgtg acagtacccg acgcactgcc ggatgtggtc actgttgtgc cgctggtcgt
     9661 ggtgttggtg ggtccggcgc tggtttgtgt tgcactagtg gaagatactg ttgcactgct
     9721 ggtgacagtg acagtgtcgg tattggtctg ggttgtagcg gatgtagaag attcggtgac
     9781 aacggtaaca gacgatacag ttgtaccgga tacggttgcc gtagtattgc ttgcggatac
     9841 ggtcgaggtt tccgttgtct gcgtgccgga tgcagattcc gaagaggaac tgctgccgga
     9901 tgtcacgctg ctggaaaccg atgtggtttt tgttgtaact gttgtctcag acgaactgct
     9961 ggtttccgag ctggaggatg tcaccttttt cagtgcatcg aacatggtga taagaccggt
    10021 tgtctcatcg tatttgttcg tcttgctctc accgacttct acgttctgca ccttgccttt
    10081 caccacttca aatgtgatct tggtggtcag ttcgtagccg tccggtgcgg cggtctcttc
    10141 cagggtgtac ttgccgtccg gaatctgttt cagcagaatt gccttgtcgc cggatgtcca
    10201 ggtgatgacc ttggagccag cttcattgcc ttcgatcttg ccgttttctg cggttacatg
    10261 ggacaggtct gcgctgccct cataggtcag tttcatacct gcgccgggca gttcctgtgc
    10321 agcgtctcca ttctcgccca tctttacaat ggatacttcg gacggtgcgt ccttcataat
    10381 cactgcgttg gcagattcgc cctcttccag gccctcacgc tttgtcacgt ttccgtgttc
    10441 gtccaccgta aaggtcatgg tctcggtctt tctggtaaag ccgtccggtg gagtaacctc
    10501 ttccagggtg tatgtgcctg ccggcagatt tgccagtgtg acaccgccct tagctgcgtc
    10561 agaagtccat gtgatggtgt tgtccttaac ctggatcgca gccacgctgt catcgccgta
    10621 agaggctttt accttggaaa ggtctgcatc cttcggtgcg atcagtttca gttctgcgcc
    10681 gtccagttcc tctgtaccgt tgatgctctg cttgccgatc gtaacgccgg gtttcagtgc
    10741 atcgaacatg gtgataagac cggtggtctc atcgtacttg tttgtcttgc tctcaccgac
    10801 ttctacgttc tgtaccttgc cgtcaaccac ttcaaacatg atcttggtgg tcagttcata
    10861 gccgtccggt gcaatcgttt cttccagggt atacttgccg tccgggagct gtttcagcag
    10921 aattgccttg tcgccggatg tccaggtgat gaccttggag ccagtcgcat tgccttcgat
    10981 cttgccgttt tctgcggtta catgagacag atctgcgccg ccctcatagg tcagtttcat
    11041 atctgcaccg ggcagtccct gtgcagcatc tccgtccttg cccaccttta cgatggacac
    11101 ttcggatggt gcatcttcca tgacaacagt cttatcatcc tttttcgtca gattgtttac
    11161 ggtttcgacc ttgccgtctg cgccgaccac aaaggtcatg gtctcagtct ttttggtaaa
    11221 gccgtccgga gcagtgatct cttccagggt gtatgtgcct gccggaagat cggtcagctc
    11281 tacgctgccc tttgtgccgt cggatgtcca gctgatggtt ttgccgtcat ttgcgatgcc
    11341 gtccgtagac ttgtcgccgt aagcataacc caccttggac aggtcaacgc cgtccggtgc
    11401 agtcagtttc agtacggctc cggtgagttc ttcgccgccg ccgactgcct gcttactaac
    11461 agaaaccgtt ttcttgatgc tgtccttcat gacgatttca ttatcagaaa cgtcatattc
    11521 ctcagaaacg gcggaagtct gtgttacctg accattcttt atggtaaagg tgaagcttgt
    11581 gcttacctta ttgtagccgt ccggtgcagc gacttcttcc atgatatatt cgccgtccgg
    11641 cagaccgatg atgtcagtct tgtttttgcc gtctgaaaca aaggtgatgg tatcggatgt
    11701 ctgcgtatac ttgccgtaat cggcgttgtc ctccagggag tctgcctttg tcagcgaagt
    11761 ttctccacgc ttgacctcca gcacatctgc gttcagactg ccgtctgtcc gggtgatctt
    11821 gatgattgcg cctgccagtt ctgcggaatc tgcaatgtcc tgcttgtcca cgctgatctg
    11881 cgaacggttg atagcggtaa ccttatttgc cgcaaaaata cagttattgt tttcaatcgg
    11941 gctgccgtcc ggatgattgg caaccacata gccgctttca tctacgctga atgtcacgct
    12001 cagcggtgcg tagcccttaa ctgcagtctc tgtcagagtg tattcaccct ttgccagatt
    12061 tgcaatggtt tccggtgctg tcaaggtctt ggaaaaaccg ccgggacctg tcagcgtaaa
    12121 cgcacagttt gccatttccc tgccgtctac atctaccttt gcaaatgaaa tctctacatc
    12181 gctctggtac gcatcggtga tggtcttatc gcccggaatg ccggcatcca taacgacttc
    12241 attgccgcca tcataggata catggaatat tttatctttg ccaacttcta tcgtgccatc
    12301 atcagcaaca cggtttccgt ccttgtccag ctcaaagacg tagtagatct tgctgctgtc
    12361 cagtccctca aaggtaacgg acttactgcc gttgtcaccg gcagtaatgt tgatgtcttt
    12421 cttttcaacc agctgatagc tctcgtctgc attctgcgta tacagtccaa cttcaattgc
    12481 atggtcaact gccggtttgc cgtcctcggt tgcgatcttc gttaccttga tccttgtggg
    12541 attttcggtg gcattggtga tgaccgcatc cgcactgggt gcatccttgg tcagcagaat
    12601 tgcctgctca ccacagccgt tataggaaac gtagaatgtc ttatctgttt tcgtagtagg
    12661 atccttgaaa tctgttacga cgctctggac aaaggatttc cctgcgccga cacgctcttt
    12721 gccgttcatc tcaaagatgt aatactcggt atcagcgtcc agaccgtcaa aggtcacgct
    12781 agagccagca ctaacagttt ttgcatcaat ctgtgtatag gtgtcaccgt tcttggtgaa
    12841 cagtcccaca gagaatgcgg gtgtgctctt gacatcatct gcgatcagca ccttgctgac
    12901 ggtgatgctg tagtcgatct cctcggtgtt ggtgatctca gttttgccgt ctgcctgtac
    12961 tgccgccggt gctgccggtt cgcaggtata gcggataccg ctgtctgagg tgtattttgc
    13021 aaagtctgta tctgccagaa caccgtttgc attggtctcc agcacgtaga attccacgcc
    13081 cttgggcaca tcttcaaatg tcacgttctg cttgcctgcc tcggtgaatg tcagagactg
    13141 taccggcgca tcttcatagc gcacatagtc cccggctgca ttcttggtga acagggcaaa
    13201 gtagaaggta tcgccggtca cgctgtcccc tgccagggtc ttggtcagcg tgatattgcc
    13261 cacatttttg gtgttggtca cgccgacagt ctggttcgcc gtggcatcgg tggaaattac
    13321 attatcggta tatttcggtg tgtaattctg aatccggttg cccttggcgt cggtttccag
    13381 gagatagatg ttgttggcgt tgttctgctg cttgatcgag gacaggtcta cgtccttcat
    13441 ggtgacggat gccgcagtct gtcctgccgg aacggtgacg gaaatcacat cgctcaaacg
    13501 tgtggtgtaa ttttcgtcag caaacagggc aaagtagaag gttgcatcct tggtctctgc
    13561 ctctgtcagc gtcttgttga cggtcacatc aatggtggtg tccggtgtct gtacccagac
    13621 gccgaccttt gccggctctg ccaccatgac ggtatcgccc agaatgctgg tattctgata
    13681 ggaatacgca aagctgttcc atgcgatatt ctcctcgccg gtctttgcca catagctggg
    13741 aacggttgca gtaactttga caacaacttc ctcaccgacc ttgacggtcg tatctttcag
    13801 ctggatacgg aagttcacgg tatccgcaga ggaattgccc gtccagttca tcacgtcatt
    13861 ctgtcccagc cagtccttgg aatattcatc caggacggag gtcttgtcgg tggagtagct
    13921 gcgtactgtt ttgtcaatta catctgttcc gctcaccaca acagaatcaa tgctcttgag
    13981 tttcacgccg aatgcggaga aacgctcata gccggaaacc agacccaagt cacccacata
    14041 gggcagacgg tcgataatgg tcatgttttc cagatctacc ggcgagttgt tcttgatgtg
    14101 gagttcatag gtcacatctt cgccctgcat accctgcaca tagttgtggg taggctggcg
    14161 gctgtaaccg gtatcggtag ccgggtccgt gtgcggtgtg ccggtgtggt tctggttggt
    14221 gtaggtaatg gtcttccagg attcggtggt cagaccaacg atgttatagt ttgcgtagct
    14281 ccagatctgg tcgtcacgct tttcgccggc aaccgtatct tcggtggaga acggctgtgt
    14341 cacactggca tagccgatat tggtgatgac gccctctttt tcctgaccgt ccttgacggt
    14401 ggtggtgaac tggaagacga tgcacttgtt cggtgcaagc gttgccttgc ccagcttgtc
    14461 cgcataaata atcagtttcg aaccgtcgat cttgtaggca tcgcggggaa caggcgtgcc
    14521 gctttccagc agaccagtct tgtcgtttgc cttaaatgtg gaactgccgt cgctgttatg
    14581 cgtcagcgtc agttcctggt aggtcatctg caagccggtc tcattgctga actggtatgt
    14641 cggcggcaga ttgtcggtca gcgtcacgcc gtccagtttc aagtctgcca catcggtgga
    14701 gtcgcctctg ccgttgtaca gcacagtctg ccagatcagt gtatcaccgg cggtgatgta
    14761 gccgttttct accggctgta cgtcatcggc attgtaggtc tgtcctgcaa aggatgccac
    14821 tgccagcttg gcgaaaccgg gtgcgggagt ggttttggtg aaggagatgt ccaccttatc
    14881 ctccggcgag atcacacggt cactgccgtc atttctgccc ttcaccactg tcaccttgga
    14941 aagggtgttt gtcagtgtga cttttttcgc cgtgctgttt gccagttcct gtgccttgtc
    15001 ggcggtcagc ttggcttggt aagtcaactt tacgccaacg ccccactgat ctgcaaattt
    15061 ttctgtggaa acaaatgtaa aggtcagcgt ctgtccaact tgagttgcct gaatgccact
    15121 caaagctgta taatttgcag ttccctctac actatttggc acatattcca tgccgtccgg
    15181 cagcgtatct tctacaacga tcgttttccc agcatagtca ttatttgaac cagaaagtgc
    15241 caagctgtag ctaaatgttt tcttctccag atctgccgct gtcataccaa cgatattggt
    15301 cgttttgtcc tcactatttg tcagtgactt tttcgcctca aaaacagtat tgcttactgg
    15361 attcaaaatc atgtcatgtg cataccaagt tacagttttg cttccgtgtt cgtttacagt
    15421 cagaccgcct actgcggtat agttatagac acctctctgt gttccgaact ttgtttcaaa
    15481 tgcacttaat gtctgcatca atgcactatt attcagatta gtaccatcac catacccctg
    15541 aattcccctt ttttcagaag cgtagtcatt atttgtatta gtgacatcgt ttccaagtac
    15601 cacgtattcc actgtcactg attcgccagc cgccagattg attcccataa tattaaaggt
    15661 ttcaaccgca tagcctccac catcaatttt attttgctct acagtaacag tctgaattct
    15721 ggggttagac gcattgccgt taattgcaac gactttcaga acatcagaag cttcccactt
    15781 caacgttgta ccataatcat ataagtacag gttttccatg gtcgtggttc cgtggttggt
    15841 aaaagtcgcc tgatatgtca gcttatcacc atttacaatt tctgttgtat catctgctgg
    15901 tataccattt actgcaatga ttttcttttg caagctatat ttgcttaaat cttcaccgcc
    15961 gtaaatagta tgcgtcgcag taccctttac ttccgcacct tcatatactt ttgcggtatt
    16021 tgtcagtgct gcggctgttg cagaatcttc cacatttgca tatactgtca aagtaattga
    16081 ctttcctttt ggaacagtaa tcgttccgcc ggcagtcaat gtttttgtat caacggaagt
    16141 aatatttttt gtggtgccgt ctgataaggt caaaatgcca tatgcaccac tattttcacg
    16201 gaatgtcatg ccgtccggca agatgtcctc tattttcaca ttttcagcat ctgcttcacc
    16261 attattcgtt actgtaattg tgtaaacaac atcttttgcc gtgccaaatg tgtaattgcc
    16321 gacatctgcc gtcttcgtga ttcccagctg tgctttcggt ctatccggtg taaacttcac
    16381 tttatttgac ggctttccgt ctacggttgc aacattggaa atttctccgt tcacgccgtc
    16441 tttgattgta ccagttaccg tgatctgtac tgtctttcct gccggaacgt caattttgcc
    16501 cgggaaatca gccactgcag caccatctac cttataagtc atattttcca gatactgcag
    16561 caagttcgca tcagaaacag caacatcttt cgcatccttt gcaccggaat tggtgacaga
    16621 aatggtataa gtagcagtct gcccggaaat gtctgtgtag tacttctgtc ccaggttctt
    16681 accattatag ataacagaac cgctcttcac cggcgtttcg atcacagcct catccttttt
    16741 cacctggatg gtcttttccg ccttttcgcc gtggtttccg gtaacggtat tggtcagcgc
    16801 cacggtttcg tccttgtcca cggtacactc gatttccttg gtgacgctct ttccggcagg
    16861 cacggcaatg cctgtccagg caatgtcccc ggtcggtgtc agtccgtccg gcagggtatc
    16921 cgtgatatcg atatcaccat ccatatcgcc ggtgttggtc acggtaatga caaacttgac
    16981 cttatcgcct tttaagtagt atgccttttc attttccacc gttttgctca gactgaactg
    17041 cggtcccttg ggtgcgctga ctgtcagttc cacgctgttt ccgccgatgg acttttccgc
    17101 tgcattgtcc ccgtagggct tgccggaaac gctcagatcg ttggtgacct tgccgtctgc
    17161 tgtaccgttc atctttacgg tactgctgtt cagcggcagt gccacattca gacgcactgc
    17221 cggcatctcg gcgttctcgt tcttgctgtt cagcacaaaa gagatgtctg cctttgcgcc
    17281 ttttacggtg acggtgggtt ctgcatcata gcctgccgcc tggatcgccg actttacggc
    17341 gttccggatg gcgtcttctg catcgccctg gaatgtcatg ttttccaggc tgatcgtatc
    17401 ctggaaagtc aggctcttca cccactctgc gccttttttc tgggacgcat tgttggaaga
    17461 ggcgctgagc gtatagttca ccgaagtgcc gcctgcaatg tcgctggctt tcagactggt
    17521 gcggtcctcg ctcttggacg cattccagtt catggctgca attgcggtga tcgtattgga
    17581 cacgctcttt ccggagcctt cctgcgagat acgcacctct gcgacggtgc cgttcggcgt
    17641 tttgccgttc cggaacttcg cctggagccg gatctgcttg gtctgaccgt tgtcaatgct
    17701 gaatgtaata taccggctgc cgtccggatt gacgtgcagt acatatccgt tatacactgc
    17761 gccgtcccgg aaaccgtttc cggcaaagtt gggcagcaga agattctggt cggtgatctc
    17821 gatccgatag gtgtcttctt caccctcatt gacattgttt ccggagatcg tcgccatcag
    17881 atagaacaga tcgccggatt ctacgccttc tttgcctttc tcaggatcag gaatagggtt
    17941 gtacttactg tcccgaaatt ctatgctgga atttgtcaat accagcggtc cgctcgcttt
    18001 cgcattttct gccgccgacg caaagaacgc cgacggaatg caagtcacga tcatcaaaac
    18061 tgccagcaag acactgatga ctcttgtacg caggtgtctt gtcttttctg tctgcataaa
    18121 aaaaactcct ctcattgcgg gaacgctgca cgcagtctgt tctgcattct ttcgtgcaaa
    18181 ctttgtacgg cgtttcctca gattttttgc accgtggcag caacatcgac aaaaacgata
    18241 tacataccac attttttacg gcaatttcca ccaactccgc cgctttcagc tgttcaattg
    18301 tttccacaat caaaagaaca ttttggcaca aatcacggga atcgctctga tacaacttta
    18361 ttatactgat tatccgtgaa attttatatc attttttata taatatttga aaagcaagag
    18421 gaagaatcag gaagtctatt caaacggctt tctgattctc caaattttgc agcggcatct
    18481 ccggcgaatg gctgtccgaa tttacccgca tgcaacttct ttcaatcaca tatgacgaat
    18541 cccgccattg ttttttcaaa tatttcgtga aaaaagctat tgcaacatcg accaaaatcc
    18601 ggtataatag tattagatat gttccaacct ggcggagcag tctgtattat ggtggttcgg
    18661 cgcaccataa agaggtacga atgggattcc ggcacggttt ctgtgtgccg ggaacgagaa
    18721 gattccgccg aaactgttga ggaggataac atggcagtaa aaacaactgg aaatccccag
    18781 agagaagaat tgaaacagaa atttgtagac agacttcagc tggagtgcag tgtttctccg
    18841 gacgaggcat ctgataaaca gatctatcag gtgctgtccg cactgatggt agaaaagctg
    18901 aagcacaagc gtcagcactt tgtaaacaaa accaattccg taggcggcaa gagcgtgtac
    18961 tacctgtcta tggaattcct gatgggacgt tccctgaaga ccaccctgta caacctggag
    19021 ctggcagacg atgttcgtgc catcctgcag gactatcaca tcaacctgga caacatcttt
    19081 gagtgcgagc cggacgcagg tctgggcaac ggcggtctgg gccgtctggc tgcctgctat
    19141 ctggacgcta tggcaaccca ggagatcccg gcaaccggtc actccatctg ctatgagtac
    19201 ggtatcttca agcagatcct ggaggacggc tggcagaccg aggaaccgga caactggctg
    19261 ccgggcggca gcgtctggct ggatccgaag ccggacgagg cgatcgaagt acacttcgag
    19321 ggcaatgtcc aggaatactg gcaggattct taccactaca tctcccatgt caactacaac
    19381 actgttatcg ccatcccgta tgatatgtat gtctccggct atggcagcac cggcgtttct
    19441 accctgcgtc tgtggaaggc acaggcaccc agctttgaca tgaaggaatt caacgagggc
    19501 aactatgaac aggcactgtc ccggaactcc atggcaaatg cgatctccaa ggtgctgtac
    19561 ccgaacgaca accacctgga gggcaagtcc ctgcgtctgc gccagcagta tttcctgtgc
    19621 gccgcagcca tcggtgatat cgtaaaccac cacatgaacg tatacggcac actggaaaac
    19681 ctgcacgaaa aggttgccat ccacatcaac gacacccacc cgactctggc aattccggaa
    19741 ctgatgcgtg tcctgctgga cgactgcggc tatacctggg atcgtgcatg gcacatcgtc
    19801 accaacacct tcgcttatac caaccacacc gtaatggctg aggcactgga aaagtgggat
    19861 gtaaacctgg taaaacagat cgtgccgaga atcttccaga tcatcgtgga gatcaacaac
    19921 cgctactgcg caggtctgat ggagcgcaac ggcggcgaca gcgcaagaac cacacggatg
    19981 tccattatca aggacaacca gatccacatg gctaccctgt gcgttatggc atctcacagc
    20041 gtcaacggcg tttccaagct gcactcggag atcatcaagg acagcgtatt ccacgaagaa
    20101 tatctggaca ccccgaacaa gttcaagaac gtcaccaacg gtatcgcata ccggagatgg
    20161 ctgtatcagt ccaacccggg tctgacccag ctgctgcgtg acaagatcgg cgacggtttc
    20221 ctgaaggacg gcagcgagct gaaaaagctg gacgttttcg ctgatgacaa ggctgttctg
    20281 gaacagctgc gcaagatcaa gaaggaaaac aaggagcgtt tcgccaagtt cgtcaagaaa
    20341 aagagcggtc tcatcctgga taccgacagt gtatttgatg cacaggtaaa gcgtctgcac
    20401 gaatacaagc gtcagcatct gaatgcaatg cacattctgg cacagtacaa cgccctgctg
    20461 gaaaatccgg atatggactt tacgcccaag acctacatct tcgctgcaaa ggcagcgccg
    20521 ggctattatc tggcaaagca gatcatcaag atgatctggg cgctgtctga ggagatccgc
    20581 aagaatcccc gcatctccga caagctgtcc gtttacttcc tggagaacta ctgcgttacc
    20641 atgtccgagc tgctgatgcc ggcttgcgat atttcggagc agatctctct ggcaggtaca
    20701 gaggcatccg gtaccggcaa catgaagctg atgctgaacg gtgcgatcac actgggtaca
    20761 ctggacggcg caaacatcga gatcggcgac gcagtgggcg ctgacaacat cctcatcttc
    20821 ggcatgaaga ccgaggaagt caacaccctc aaggctcgtg gctatcgtcc ggaggactat
    20881 tacaacaaca acccgatcat caagagctgt atcgaccgga tgtatgccgg catcaacggc
    20941 tgcaagttca cccaagtggc tgactccctg cgcacccagg atccgtacat ggttctggct
    21001 gacttcgaca gctactgcca ggtacaggag cgcagttcaa agctgtacac cgacaaggat
    21061 acctggacca agatgtccct gcacaacatc gcaaacgcag gcatcttctc cgctgaccgt
    21121 gcagttaacg aatatgcaaa ggatatctgg aatatctgag aacgtgttat cacacattct
    21181 gatataatct gacgaaaacg gagcagcaca aaccctgctc cgttttcttg aatgggatgg
    21241 gttgtgtgag gtatccctgc tttatattgt agtgaaaact atagaaataa gccttccttg
    21301 aaccagcgaa gctggtaaag gaattacaat gaatttttcc ttatctgcaa atttcacaga
    21361 aaaaatccct cagtcagcct gcggctgcca gctcagcaca aagcacgccc ttcgggtgcc
    21421 ttttcaaggg agcctaacct aatacaggaa gtatcccttt gaaaagaata ctctcccact
    21481 ccgcaaagaa aggagcatct ctgtatgcgt tatttttaca acagttggga tccggcgtat
    21541 aagtcgcact tcggtgcgat cgcccggaac cagagctgtc agttcaccat tcgcctgccg
    21601 gaagaaacgc cgctggactt ttctccggtg ctggtggttt tccgcaccgg tttcaaagag
    21661 cgttttctgc ccatgaacgt gaaaacccat gaaaacggct acatcaccta taccgccgac
    21721 cttatcccca agtattccgg cgttcactac tattatttct cctatacctc caacggcgtg
    21781 cgtcactata tcaagcgtgg cgatgcctgc catggtatgc tggatcaggg cgaaatgttc
    21841 cagctcaccg tctacaacga ggactatcag acaccgcagt tcctcaaagg cggcgtgatg
    21901 tatcagatct tccccgaccg tttttgcagc agcggcaagg tgcatgacaa tgtaccgtct
    21961 gaccgtgtca tgcggaactg gggcgagatg ccctactaca agcccgacca gaatggtcac
    22021 gtctggaaca acgactattt cggcggcgac ctggacggca tccgcagcaa gctggactat
    22081 ctgaaagacc tgggcgtcac ctgcatctat ctgaacccga tcttcgaatc ccacgagaat
    22141 caccgctata acaccgcaaa ctaccggaat gtagacccgc tgctgggtac caacgaggac
    22201 ttccgggagc tgtgcaaggc ggcaaaggta cggggcatct ccgtcatcct ggacggcgta
    22261 ttctcccaca ccggcgcaga cagcatctat ttcaacaagt tcggacgcta caacacgccc
    22321 ggcgcttatc agtccgagaa cagcccgtat tactcctggt atcacttctg gggcaacaag
    22381 gagtacgagt cctggtgggg catcgacacc ctgccgaatg tggacgagaa caaccccagc
    22441 tacaccaagt acatctgcgg cgacgacggc gtactggact actggatgag cctgggcgcc
    22501 gccggatacc gtctggacgt ggcagatgag ctgccggacg agttcctgga caatctgcgc
    22561 acctgcgtca agaagtatga tgaggaaaaa atcgtcatcg gcgaggtctg ggagg
//
LOCUS       DAWENO010000015        22093 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_982_length_22093_cov_11.569879, whole genome shotgun sequence.
ACCESSION   DAWENO010000015 DAWENO010000000
VERSION     DAWENO010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 22093)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22093)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22093
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_22093_cov_11.569879"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15984..15993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4658 a         6051 c         5281 g         6093 t
ORIGIN      
        1 ccttgatgcc ggcacccagt cttgcctgat agtacatatt gtagaacata tcagtggagt
       61 caaccttacc gttcaggtct gtatcgccct tgatgccgat gtaaacctta gcagttgcaa
      121 ccttggtacc cttgtacagg atatccaggt cgtttgctac ataagccttt gcaccgtgca
      181 gcttctcgta catttccttc ggagttgcat aagcgaaggt caggtcagca gttacatcct
      241 tgccgtccag agtagcgctt gcgatcagat cggtcttagc gaagtcacga gcatcctcag
      301 acaggtagaa gtttgcatct tcctttacct taacatccgg cttgccagca ggtgtggtgt
      361 cagaagaaac cttagtggat gttgtaccta cagaagactc agtgcttgct gttgtgtcag
      421 aagaaacagt agtggactca gtggttgttg cggatgtttc agctgtagta gttgttgtct
      481 tagctgcagt ggttgtagtt gcagcggttg ttgtggtagt tgcagcggtt gttgtggtag
      541 ttgtgccagc agttgtggtg gtagtagtaa ccttagcaac ctttacgcag atgtaaccat
      601 tttccagagt tacgttctgg ttgaagtcga tcttgttgcc tgctgcgtca gtgtaagcga
      661 agcggtcaac agcaacttct acgacattgc cctgaccatc cttggagtag ccagtgtctg
      721 tgccgtattc tgcccagtta accgggaaca gatagccgcc ctgagcttca tcatattcca
      781 ggttgtactc tgctgccaga gcctgtacag ttgcttcatc agcgatgccg atcggcagct
      841 ggaacagtgc gccgtctgct gcagtcggag cagtgtagga atccttgtcg gtgaatgcga
      901 agtggagagt cttgtttgct tcatcgttcc agaacttagc gaagtctgtg atgaaggtcg
      961 gcatagtctc gtatacgctg ccgtctgcgc cgtaaacctt catggtgtcc tcaccatacg
     1021 gccagcccag gagcttcttg gtagcatccg gcagttcgat agtaccctgg agaccgctgg
     1081 tcttgatcgc attcttgatt gctgcgccgt acattacgtc gctgtcatag cctgcgcccg
     1141 gttcaacggt gatcttatcc agagaccaaa cgatcttatc cttgatcggc tctacagtga
     1201 ttgtagtagt cgttgcagat gttgtggtct gagcagcact tgttgtagta gcagatgttg
     1261 tggtctgctg tgctgttgtg gttgtggttg cgcttgtggt agttgtggtt gtggttgcgc
     1321 ttgtggtgct tgtggtagtt tcagcagaag tagtggttgt agtctctgca tctacatata
     1381 ccttgtatgc accagttaca tagtttacgc gagttgcgtg gatatcgttg ttgttttcgt
     1441 cacgatagtc gaaacgctta acttcaactt cctttgtgcc ttccggagtt acgctgtagc
     1501 cgatgtctac gccgtcttcc atccagttaa ccgggaactc atagtaagag cccttgtcgt
     1561 cagacttcag ttccagacca gcctcagcag ctgctgcctt aacagtagcc tcatcagcaa
     1621 tgttcatggt cagagtagcc agcagatcgc cggacggatc gaacttaccg gattcgctgg
     1681 agtaagcaaa gcccagacgg ccgttctcag cgtattcctt ggtgttcata acagccttgg
     1741 acaggtcaga ataagcatcg cctgctacga agcagtttgc catcttgtag ccttccggca
     1801 tttccaggat cttacgagta acatccggaa ccatcattgc accaacgaca ccgtcggtct
     1861 tgataccgtt cttcagagtt acgttcagtt caacttcttc atcgctcggc ttgcagtcaa
     1921 ctgtaccgat ttcccaagtg atgtcctttt caacaaccgg tgcatcggtg taaaccttaa
     1981 agccgccggt tacatactta acagaagtgt ggatatcgtt gttgttttcg tcacgatagt
     2041 cgaaacgctt aacttcaact tcctttgtac cttccggagt tacgctgtag ccgatgtcta
     2101 caccgtcttc catccagtta accgggaaca ggtaataagt acccttctcg tcgctctttg
     2161 cttccagacc agcctcagca gcagctgcct taactgtatc tgcatctgca atgttcatgg
     2221 tcaggcttgc cagcaggtcg ccagccggat cgaacttacc ggtttcgctg gagtaagcaa
     2281 agcccagacg gccgttctca gcatattcct tggtgttcat aacagacttg gacaggtcag
     2341 aataagcatc gcctgctacg aagcagtttg ccatcttgta gccttccggc atctccagga
     2401 tcttacgagt aacgtccgga acctggatcg caccaacgac accgtcggtc ttgataccgt
     2461 tcttcagtgc tacatccagc tcgacttctt catcagccgg cttgcagtca actgtaccga
     2521 tctcccaaac cggaccgcct tctgtcggag tctcagaagt ggtggttgtg gtctggctct
     2581 cagtggttgt ggttgcgctt gtggtagtag aagattctgc cgggccaacg ccgtcgccga
     2641 caacgatctt tacgattgcc ttagacgcat cctgcggcag agaataggta gaagttgcct
     2701 tcttgccgtg gatcgcagtg gacatatctt ccagtgtgcc gcttgcacgc tttgcatcct
     2761 gttcagcctt taccgatgcg aaatcagcag cggtacgcag cggaaccagg ttcttgtcag
     2821 catcacggct gcagatctgt acatagtatg taccctcttc tgtacccttc gggataacca
     2881 catcgaagga tgtgaaacga agtgccatag aatcgccggt tacaaacttt gtacggtcag
     2941 aactgtcagc agcgataaag ccccaggaat agtagttgtt gatatccggt acgcagtcat
     3001 caccgtctgt gttctttacg aaacgagtgg tggtgtagca cggcagttct tcaacaactg
     3061 tcttttctac atagtttgca gcaacttcag caccctggtt gatgtagctg taagtgatgt
     3121 acggaacgcc gtcttcgttc gtcagaactt cgcattcctg tgtcttcttg gtcttgtgcg
     3181 tagccttgtc tgcgcccttg tcagccagat aatcgtctac atttgcatag tactcatatg
     3241 taaacttaac cttgttgtcg ccgttggcta ccaggtcagc gccgaagatc ttatcgcagg
     3301 cgatggttct tacgacaccg ttgctctgtg catcggtata gctccagccc ttcttgctct
     3361 tgaacatcgg agccaggcag aatacaccat tataaacggt gtttacggta gaaccgtccg
     3421 gcatagcaac ttcgtgcttt gcacccttat cctgtgcatt gaaaacattc tcataataga
     3481 tcttgttgtc aactgcaccg ccgtcagcga aagtgctgcc cttggtggtt acccagttca
     3541 gggttacctg gttcagatag ttgtcatcag cccattcatc agagtcaatg aaaacatcca
     3601 gagtcttggt gacattgcca gcgtcaacgt cgtccttgct tacctggatc tcctgtacgc
     3661 cagttccctg ttctttcagg acaaagtcat acggtgtgcc gccgtttgcg gtctggtcct
     3721 tataaaccga agccgtaacc gacaacgcac tgaacggcag tgcagagact gccatcagag
     3781 ctgcaacgcc gaaggataat accttctttg ttcgtttctt catgttttcc tttcctttcc
     3841 ttgccggcgg tttgccgaca aatacgtaaa tgaaatattg cctataatca ggagaccctt
     3901 tcccctcgga gagcgtctcc caaatataca aaatttagag gcgctgcccg tgatacagct
     3961 tattctgtat acggcagtgt gttgatgatg gataccagga actggagcag cactgtgctg
     4021 tcagaaccgg tcacttcgcc gtcgccgttg cagtcagcgt tctttctctg aaccgcattg
     4081 tccagtgtaa ctgcgcctgc tacgaccttg ttcagcataa cagcatctgt gatatccaca
     4141 cggccgtcgc tgttgacgtc gccgtacagg acgctgcctt caactgtacc gccgctggtc
     4201 tcgctgccct tgctgtcgct ggttgccgga gcctcagatg cagtggtctt ggcagtggtc
     4261 ttagaagtgg tctcagtagt atcctcgccg gaagtggtga ctgtggtctc ggtggtcgtc
     4321 tgaccggact tgccgacggt cacagaacca gcctctgttt taatggtgta ggatgcagca
     4381 tccttgccgt ttacatagcc gatgaccatg ctcttgttgt tctcgccgga gccctgatag
     4441 gtctcacggg tgaccggagc gaagtcgatc ggggtaactg tgccgtcctt cacatcgtcc
     4501 ttgaccgtac cgctgatggt cagaattacg ccgtcctcag cgatccagga tgcagaaccc
     4561 agcgcagtag accagctgac agtgatctcg ctgtccttga tgttggtgtt gaacaccggc
     4621 gcatctgcgg ctgtgctgtc gccggaaaca tcaacgtccg cagacttgcc gatcttgacg
     4681 ctgtctacat tcaggacagt gttgtcatag gttacggcga agtccattac gttgatcttg
     4741 gtggacggga tgtctgccag agatacatct acagaaaatg cgccgccagc ctcagcctct
     4801 accttggcac ccttcagggt tacagatgcg tccgcagccg aaacctgaaa agaaacagca
     4861 gtagaagcaa gcattgtaag tgccaaagcg ccggcaagtg tttttgttgc cttcatgtgt
     4921 tatcctcctc tgaattgttt gcagccccgc cggtgttccg ggccggcgct ctgcaaatga
     4981 taaaatttgg tcaaaatctc cgcaggaatc gcttctctga ttcatgctgt tccaattatt
     5041 cttattatag aatatcccaa cagaaaagtc aatagatacg caaagttttt gtgtaaactg
     5101 gctgaatcgg gaaagctata attgtataat atcccgatac gccggaaacg ctgcaccgga
     5161 ttttgaaaaa aaggcggtgc agcatcgcaa aaacaagggc aaaaaccgac tcagacagcc
     5221 gccgacgcca tgctgccgga gcgtgctgcg tacagaaatt ccccggtatc gggcggcatt
     5281 tctttctgcc ggatctctgc gctgcgtgac aggcagaaac cttcattcaa attatactct
     5341 aaaactcgca aaatgtaaag agacatacgc attttttagt acattcacac aaaaactata
     5401 cttcattttt atgatttccc acaaaaatca tcgtcgaaat tccgcaaaag ctgccgcccg
     5461 ttctgcaccg gagctgacat ctgccggggc ggagaccctt ttccacaatc ttttcccttt
     5521 tgcacagcca attgtggaaa acacttccgg cgaaaccggt gcaccgtttt ccacagtgtg
     5581 gaaagtgcat tgccgcccgg ttcggtgcgg atttccacaa agctgtgcgg ttggttttcc
     5641 actttgcgga gtcttctgct gcggcggtga atgcgttcca gacagaaaaa caggcggagc
     5701 atccgggtga gggataactc cgcctgttct tatcgcagca catcagcgat gtgccgaatc
     5761 acattacatc ttaaaccagc ttgatgatag ccatttcagc ggcatcgcca cgacgagggc
     5821 caatctttac aatctgggta tagccgccct tgcggtcagc atacttcggt gcaatttcgt
     5881 cgaagagctt cttagcgacg ctctccttgg ttacatatgc caaaacctgg cgcttcgaat
     5941 gcagatcatc cgacttagcg atggtgatca tcttttcagc aactgcacgt acttcctttg
     6001 cacgagtgac agttgtttcg atctgaccat tttccagcag gaatgttacc attccacgca
     6061 gcatagcctt tctgtgatcg gtcggtctgc ccagttttct ggtacccggc attgttattc
     6121 actccttttt ctagctgtag gcgctccggc ggggagaacc cacaaggctc ggtcttgttg
     6181 aacgtctggt gttttacgtg aaccagacga ttagtcttcc tcactggcga gatcaaagcc
     6241 cagagactgg agcttttccc gcacttcatc gaaggacttc ttgcccaggt tgcgcacctt
     6301 gagcatttct tcctccgact tgttgatcaa atcgtccacg gtattgattc cggcacgctt
     6361 taagcagttg aatgaccgaa cggacagatc cagttcttcg atggtcattt ccagaatctt
     6421 ttccttaccc ttgtcatcct tttcgacgag gacctcggcg atgcttgcct catctgagag
     6481 gtcaataaac agcttgagat gctcgttgag gaggtttgct gcctgagaga tcgcttcctt
     6541 tgcagagatg gtgccgtctg tccacacttc cagtgtgagc ttgtcatagt cggtgacctg
     6601 acccagacgg gtgtcctcta cgtggtagtt tactttcagg actggggtat agatactatc
     6661 tactgcgatg acatcaattg gagtattcat catctgcttg ttgcgctccg cagagacata
     6721 gcctctgcca cgatcaatga cgatttccat atacagcttt gcgccttttc ccagggttgc
     6781 gatgtgcata cccgggtcga ggatattcac ttcagagtcg gtcttgatgt cgccggcggt
     6841 aacttcgcac tcgccctcgg cttcgatata aaccgttttc ggtccgtcgc cgtagagctt
     6901 tgcggtgagt cccttcaggc tgaggatgat ctcggtcaca tcctctttca caccgggaat
     6961 cgtactcaat tcatggtgta cgccatcaat cttcacggaa ttcacagcca caccgggcaa
     7021 tgaggagagc agcacacgcc gcagactgtt gccaagggtg ataccatagc cacgttccag
     7081 cggtgccaat acaaacttac cgtattttcc gtcgcttcgc agatccactg tgtcgagttc
     7141 cggcttttca atcttctcca gcataaaaac accctcctat gcttggtagc tttgtctgtg
     7201 gttgttcctg gcataccacg gccgcagatg ctccttggta cgtcaggacc ttgcgtaaga
     7261 tccctcagga aaccagagcc gtccggaatg ggacagcggt gatttccaaa gagatccttc
     7321 tcatgttccg taaattgcag atgattactt ggagtacaac tcgacgatca agtgtgctgc
     7381 aacttcatag tccagatctt ccggcttagc catacgaacg atcttagcgg agaagttttc
     7441 cttatccggt tccagccata ccggaacggt acggtctgcg gtctgttcaa caacgtcctt
     7501 gaacttaccg ctgtcacggc tgccttcaga cagagcgaca acgtcgcctt ccttgacacg
     7561 ataagacggg atatccacac gctggccgtt tacggtaaag tgaccatgag ttaccagctg
     7621 tcttgcttct ctgcgggtca gagcgagacc catgcggaat accacactgt ccagacggga
     7681 ttccaggcaa gcgatcaagt tgtcaccagc cttgccctcc atcttagatg cgatttcgaa
     7741 caggtgatag aactgctttt cgcctacgcc gtagataaac ttcagcttct gcttttcttt
     7801 cagctgcatt ccgtattcag aaacctttct gcggtttcct gcacccggat tgcggttaga
     7861 ctgcttggag acacccataa ccatcgggct gatacccaga tagcggcatc tcttgagaat
     7921 cggttctctg cttactgcca tgatatatta cacctcctgc tagtgtgatt agacacgacg
     7981 tctcttgggc ggacggcatc cattgtgagg gatcggagta acatccttga tcatcttgac
     8041 ttccagacct acagtctgca gtgcacggat cgcagcctca cgaccagatc ccggtccctt
     8101 tacgtatact tctaccgact tcaggccgtg ttccattgct gccttagctg cagtctctgc
     8161 tgcggtctgt gctgcaaacg gagtcgactt tctggaacca cggaagccca gtccgccgga
     8221 gcttgcccat gaaatggcat tgccctgtgt atcggtgatc gttacgatgg tgttgttgaa
     8281 tgtagactga atgtgtacag cgccgttttc aatgttctta cgttctctgc ggcgacgaac
     8341 aacaaccttc ttgcctttct gctgtgccaa aacgaaatac cctcccttac ttcttcttgt
     8401 ttgcgatggt ctttaccgga cccttgcggg ttcttgcgtt tgtctttgtc cgctggcctc
     8461 ttaccggcag acccttgcgg tgacgaacgc cacgatagca accgatctct acgagtctct
     8521 tgatgttcag agcaacttca cggcggaggt caccctcaac cacgatgtgc ttgtcaatat
     8581 agtcacgcag ctttgctacg tcctcttcgg acaggtcctt gacacggata tccgggttta
     8641 cgccggtacc tgccaggatc tcatctgctg tgctgcggcc aattccgtag atataagtca
     8701 gaccgatctc gatccgcttg ttcttcggaa ggtctacgcc tgcgattctt gccattcttc
     8761 ttgcacctcc atgttaaaaa tcccgagctt agccctgacg ctgcttatgc ttcggatttt
     8821 cgcagattac cattactttg cctttacgct taatcacctt gcatttttcg caaatcggct
     8881 ttacggaagg tcttactttc attgcaactg tcctccttct cggaacaact gtgcagccgg
     8941 acgatgccgc ctgcgctatt tacgatggaa aacgacattc cggagaattc cggaaccggt
     9001 ttccctgtgc gttatctgca caattacttg gctctccaag tgattctgcc cttggtcaga
     9061 tcatagggcg acatctcgac ggtcaccttg tctcccggca cgatgcgaat gtagttcatc
     9121 cgcagcttac cggaaacgtg tgccagaatg gtgtgcttgt tttgcagctc caccttaaac
     9181 attgcattcg gcaaagattc gagaacgacg ccttctacct caatgacatc ctgctttgac
     9241 aaattgataa cctccccaat gggtatcttc ggggcatatg agtcactgct cacacgcctg
     9301 atattgtttc aacaaatttc tgagcttttt gtccgtcacg ccgtccagtg cgatgcactg
     9361 gttcgtccgc tggagatggc ggagattctt ccgcttgggc gacgccagcc ggcgccgctt
     9421 tccgtctgcg atctcggcat agccgccgtc cacagacagt accacgaaaa atcctcccat
     9481 atcccgtccg gcgagcgcac ggaccaccag acctctcgtt atttccatcc ggaaacctcc
     9541 gttacggtct ggttagaatc accggtccat cctgcgtcac tgcaatttcg tgttcaaaat
     9601 gcgccgcaag agagccgctg cgggtcacga ccgtccagcc gtcaccgaga acacggacgg
     9661 catcgccttt tccgttgatc atcggttcaa tgcagaacgt catgccgggc atcagccgca
     9721 gtccgtgacc gggcttgccg tagttcggca cttccggatc ctcgtgcatt tcacggccga
     9781 ttccgtgtcc gcagtattcc cgtacaatac cgtacccacg ggaaccgcaa tattcttcca
     9841 cggtatgaga cacatcaccg agacgtgcgc ccacctgtgc cgcatcaatg gcagcgtaca
     9901 ggctggcttc tgtcacacgc agcagatctg ctgcctcctc agagatatcg cccacagcga
     9961 aggtatacgc tgtgtcgccg acaaatccgt taaaggttgc gcccacgtcc acgctgacga
    10021 tgtcgccctc gtggatgaca cgcttgtgac tggggatacc gtgaatgatt tcgtcgttga
    10081 tcgaaatgca ggcacttgcc ggaaaaccgc cgtagcccaa aaaggtagga actgcgccgc
    10141 agcccacgat aaaatcgtga atggacttat ccagctgctt ggtggtcata ccggggtgca
    10201 tcacctttcc ggcatggacc agtgcgtttg cagcgattct gcctgcctcc cgcaatttct
    10261 gtatatcaga cttagatttg atcgtcacac tcattttacg cccacagcct tcaataccag
    10321 agcagtggta tcagccactt cctcctgacc gatgacggtc tcgagcttgc cctgcttttc
    10381 gtaatatgcc ttcagcggtt cagtcgtctt gtgataaacg gcaagacggt ccagcacggt
    10441 ttccggctgg tcatccttgc ggatgatggt ctttgcgccg cacttgtcgc agacgccctc
    10501 aacctttgac ggcttgaact gaatgtgata ggttgcaccg cagccctcgc agactcttct
    10561 gccagagaca cgctgcttga tggtctcgtc gggaacgaag atctccagaa ccttgtcgat
    10621 cttcacgccc atttcgtcca gcgcttctgc ctgaggcacg ctgcggggaa aaccgtccag
    10681 aatgaaacca ttcttgcagt cgtcctgtgc aatgcgttcc ttcaggatgc cgatgacgat
    10741 atcatcggag accagggcac cgctgtccat tgcagccttt gccttgacac cgtattcggt
    10801 gccggtgcgg actgcctcac gcagcatatt gccggtggaa atctggggga tcgacagtgc
    10861 ctgtgagatc acttctgcct gagtgccttt gccggcaccc ggtgcgccta agagaatcag
    10921 attcatggga atatgcctcc ttgcttattc gaggaagccc ttatgatgac gcatcatcat
    10981 ttcagactcc agcgtgcgga cggtttccag tgctacgctg acaacgatca ggatggtagt
    11041 accacccatt gccagagaac tgaggttcgg gtccatccag ttcagagcga tcgggaagat
    11101 cgcaatgatg cccaggaaga acgcacccac cagagtgatc ttggagagaa cacgctggat
    11161 atagtccgaa gtcggcttgc cgggacggat gcccgggatg ccgccgttgc tctgacgaag
    11221 gttgttggcg atttcgatcg gattatactg catggataca tagaagtagt tgaacgcaat
    11281 gatcagaatg aagtaaatca gtgcataggt ccaagttctt gtattaaaga agttgataaa
    11341 tccgttatag aacttctgcc agaagccggt ggggtctgtg tataccggct taaacaggct
    11401 gatggtgctg ggcagagaca taaaggacat ggcgaagatg atcggcataa cgccgctcat
    11461 gcaaaccttt accggaatgt gggtagactg gccgccatac tgttttctgc cgacaacacg
    11521 ctttgcatac tggatcggga tccggcgttc tgatgcgttc atgaatacaa tgaaagcgat
    11581 cattgcgatg aacagtacca ggatgcccag gctctcgaag aagtagcgct ggggttcgtt
    11641 tttggtgagt tctcccagag tacctgccat aaccgggaat cttgcaagaa tgccggcgaa
    11701 caggagcatg gagataccgt ttccgatacc cttatcgttg atctggttgc ccatccaaac
    11761 gacgatggaa gtaccagcaa taaaggacag gatgataaca aatgcgataa agacgttggc
    11821 tgccttagaa ccgccgtcca cgactgcgtt catctgattt ttcagcgtca gataataagc
    11881 aaaggacatg aacacggaca gtgccaagga aacagcagct gtgattttgt tgagcttttt
    11941 ctgtccctct tcgccctcat cacgcatacg ctccagcgga ggcagtgcat aagtcagcag
    12001 ctgcatgatg atagaagcgt tgatgtacgg ggtgatggac agagaaaata cggttgcctg
    12061 agacagcgca ccaccggtga tgatatccag gtattccagg aagttaccgc cggttgcgtt
    12121 ggatgccatc caggtgctta ccgtagaggt gtccaggaac ggaacgacta cggcgcttcc
    12181 gagacggaaa atcaggagca tcagtaatgt gtacaggagc tttttccgga tctccggaat
    12241 actccaagca tctctcaacg tcttgaacac gttacatcac ctctgctttt ccgccagcct
    12301 tttcgatttt ttcgactgcg ctctttgtga actttgcagc cttaacagtc agcttggttg
    12361 tcagctcacc gtcaccgagt accttaacgc cgtcgcaagc ctttttgatc aggccggctg
    12421 ccttcagcag ctctgcgtca acgacagtac cctctgtgaa cttgttcaga tctgaaacgt
    12481 ttacgatagc gtattccttt gcaaagatgt tgttaaaacc tctcttcggg atacgtctgg
    12541 tcagcggcat ctgaccgcct tcaaaaccga tgcgtacgcc gccgccgctg cgggcgttct
    12601 gacccttgtg acccttacct gcggtcttac cgttaccaga gccgtggcca cggccgatac
    12661 gctttacttc ctttgtcgag cccggagccg gggacaattc gtgaagcttc atgctattgc
    12721 acctccttag atattaactt atgcctctgt tacctctacg aggtgagaaa ttttctgaat
    12781 cttgccgagg gttgcctcat tttcaggctg tacgctgaca tcgccgacct tcttcagacc
    12841 cagcgaattt gctgtagcaa tctgatcctt cttgcgaccg atcaggcttt ttacgagctt
    12901 gatattcttt gccatttcgc accctcctta tttatagatc tcttttacag tcttgcctct
    12961 cttttcagca acttcagctg ctgtagtgca agaagccaga ccgttgatgg ttgcacgtac
    13021 cacgttgcac ggattgttag agcgcagcga cttggtacgg atatccttga taccagcaac
    13081 ttcgatgact gcacgaaccg gaccgccggc gataacaccg gtaccgggag cagccggctt
    13141 catcaggacc tcgcctgcgc cgaactttcc gacgatctcg tgcggaatgc tggtgccctt
    13201 cagcgggatc tggatgagat tcttcttggc atcttcaatg cccttgcgga ttgcgtccgg
    13261 aacttcgccg gacttgccga gaccgaagcc cacgataccg tttccgtcgc ctactactac
    13321 cagtgcagca aacttcatga tacggccgcc ctttacggtc ttggatactc ggttaatgga
    13381 aacgaccttt tcagccagtt ccagtccctg cggatcaatt ttagccaaag taatacctcc
    13441 cctgttttag aacttcagtc cgttttctct tgcaccctca gccagttcct tgactctgcc
    13501 gtggtacaga tagccgcctc tgtcgaatac aacatcagtg atgttctttt ctgctgcacg
    13561 ctttgcgatc atttcgccga ccttgcgtgc gccttcaaca ttgccgcctt cgccttcaaa
    13621 tgccttatcc atgctggatg cggatgccag ggtcacaccg ttgacatcgt cgatcagctg
    13681 tgcatagata tgctttgtag aacggtaaac gcacagacgg ggacgctcag gagtaccgga
    13741 gatcttcgac cgaactcttg cgtgtctttt tgctctggct ttcttgctat ccatcttttt
    13801 aatcatagcg gtttactcct ttctgcttac ttcttgccct tgccggcttt accttccttg
    13861 cggatgatac gctcgtcgac atagtggatg cccttgccct tataaggctc cggcggacgc
    13921 ttttcacgta cttctgcggc aaactggccg accttctgct tgtcgatacc acttacgacg
    13981 atggtattcg gctgcggaac ttccagcttg atgtcgtcat tttcggtgac gataacctgg
    14041 tgagaatagc ccaggttcat aacgacgtcc ttgcccttct ttgcggcacg atagccgaca
    14101 ccctcgatga tgagggtctt ggagtaaccg ttggttacgc cttcgatcat atttgcgatc
    14161 agtgttctgg tcagaccgtg cagagagcga tgctctttgt cgtcagtcgg acgagttacg
    14221 atcacttcgc cgttttcaac tgcgatgccc agttccttcg agaactgtct ggacagtgtg
    14281 ccctttgcgc cctttacggt taccaggttg tcttcgcact ttacttcaac gccagaagga
    14341 acactaaccg gtttttttcc tattcttgac ataatcccgt gtcctcctta ccaaacgaat
    14401 gctaagactt cgccgccgac gttcagctca cgagccttct tgtctgtcat aatgccctta
    14461 gaggtagaaa cgattgcaat acccaatccc ttgcgtacct tcggcatatc ttcacagctg
    14521 gaataaatac gcagacccgg cttggaaacc cggcgcagac cggtgatgac cggagagcgg
    14581 ttgtctgtat acttcagtgt gatgcggatc acgccctgct tgccgtcctc aattacctga
    14641 aagcccttga tgtagccttc gtccaacagg atctgggtaa ttgccttctt tacattggaa
    14701 gcgggaacgt caactgtggc gtgccgtgcg gaactcgcat tacgaattct tgtcagaatg
    14761 tctgctattg tatctgtaat ctgcatgcca atgacctcct tttcgtagaa ctccttttta
    14821 ccagcttgcc ttctttacgc ccggaatctt gccctcatga gccagttcac ggaagcaaac
    14881 acggcagatg ccgtactttc tcagataaga atggggtctt ccgcaaatct tacaacgggt
    14941 gtaagcacgt gtggaaaact tgggagttct ctgctgcttt aatttcattg ccttttttgc
    15001 catgactgtt tcctccctga ttacttcgca aacggtgcgc cgaggagaga cagcagttct
    15061 tttgcttcct catcggtctg tgccgttgtg atgaagttga tatccatacc tcttaccttg
    15121 tcgatcttat cgtactcgat ttccgggaag atcagctgtt ccttgatacc ggtagagtag
    15181 ttgcccttac cgtcgaagga attgccgttg attccacgga agtcacgtac acgggggaag
    15241 ctgacattaa acagcttgta tacgaactca tacatctttt cgccgcgcag ggtaacttta
    15301 acgccgatcg gcataccctc acgcagtttg aagttagcga ccgacttctt tgcacggcaa
    15361 acgaccggct tctgaccggt gattgctgcc agatctgtca tgatcgcatc gatcaccttt
    15421 gcattttcct ttgcttcgcc ggctgctacg ttaacaacaa ccttgtcgag cttcgggatc
    15481 tgcatgacat tttcatagtt gaatttcttc atcattgcag gagcaacggt gctattgtaa
    15541 taatctttca gcgtagacat atcgtttctc ctttactcaa aagatttgcc gcactttttg
    15601 caggtgcgca gctttttgcc gtcctcgatc acgtgaccga ttctggtggg cttgccgcac
    15661 ttcgggcaaa ccagctgtac cttagatgcg tacagtgcac cttcggtctc gacgatgccg
    15721 ccgacctgac ccatacgagt gggctttacg tgcttggtta ccacgttgaa accttcaacg
    15781 ataaccttgc cttccttcgg gctgactgct acgaccttgc cggtctttct gcccttggac
    15841 ttatccttcg ggtcattgta cagatattca tccttaccag tcagcaggac aacggtgtca
    15901 cctgttttta cgtgtacctt actcatcttg acacctccgc cttacagtac ttccggtgcg
    15961 aggctcagga tcttcatgta atcnnnnnnn nnnacgagtg cccttcgggt ttttgtcatc
    16021 acgaataata acagcagcgt tctcatcgaa acggatatag gttccgtctt ctctgcgaag
    16081 gcccttctta gaacgaacga ttacggcctt gaccacatcg cccttcttaa ccacgccgcc
    16141 gggtgttgct ttcttaacag atgcgactac gacatcgccg atattggcat atcttctgcg
    16201 agtgccgccg agaactctga tgcacatcag ttctttcgca ccggtgttat ccgcaacctt
    16261 catataagac tgcatctgaa tcacagcgag ttcctccttt cagaatcgct tccgacctta
    16321 tcggaaaccg ttctgtttgt actctattga tgttttcgaa ttactttgcc ttttcgagga
    16381 tttcgatgac acgccatctc ttgtccttgg acagcggacg ggtctccata acctcaacac
    16441 ggtcgccgac tctgcactcg ttcttttcgt cgtgtgcctt caggcgaacg gttctcttga
    16501 caatcttctt atacagcggg tgttttacat tatcgacgat agctacaacg acagtcttat
    16561 ccatcttatc gctgacaacc aagccgactc tggtttttct caaatttctc tcagccacag
    16621 taaaaccctc ctacttactg ctgtgcagac agacgcagtg ctgtctttac acgagcaatg
    16681 tcctttttaa cagccttaat gcgagctgta ttttcaagct gatttgctgc gagctggaaa
    16741 cgaagtgcaa agagctcttc cttcaagctg tccagctttg cgttcagctc atctaccggc
    16801 atatttttga tctcggttgc tttcattact gctcgcctcc tttttcagcc tttacaaact
    16861 tacacttgat cggcagcttg tgcatagcca gacgcattgc ctcacgtgcg gtctcttccg
    16921 gaacgcctgc gatctcgaac ataactctgc ccggcttaac gactgctacc cagtactccg
    16981 gagcgccctt accggaaccc attcgagtgc cgagcggctt ctttgttacc ggcttatccg
    17041 ggaaaatctt gatccaaacc tgaccgccac gcttgatata acgagtcata gcgatacggg
    17101 ctgcttcgat ctggtttgag gtgatccatg ccggctccag tgcctggatg ccgtactcac
    17161 cgttgcttac gaaattacct ctggttgcct tacccgtcat acgtcctctg tgaacacgac
    17221 ggtattttac tctcttagga agcagcatta ctggttacct ccttctcttc tagcgtcacg
    17281 gcggaaattg ccgccatttc tgcggtcacc gttccggttg tcacgacggc ggcggtcatc
    17341 tctgccgcca cggttgttgt cccgggactt tctctctacc ttttctctct gagcggcagc
    17401 cttagcagcg tcacccttga gaacttcgcc cttatagatc caaaccttta cgccgatgcg
    17461 gccataggtg gtgtgggctt ctgcgaaacc atagtcgata tcagcacgga tggtctgaag
    17521 cggaatggta ccttcgtgat agctttcgga acgagcgatt tctgcaccgc ccagtctgcc
    17581 ggaaaccttt gtcttgatac cctttgcgcc caggcgcatg gttctgccga tggactgctt
    17641 cattgcacgg cggaaggaga tacgattctc cagatccagt gcgatctttt cagctaccag
    17701 ctgtgcatcc atatccggct gctttacttc tacgatgttg acataaacct gcttgttcag
    17761 cattttttcc agctgcgcac gcagtttttc gatctctgcg ccgccacggc cgataacaac
    17821 gcccggcttt gcgcagtgga tgtggattct aaccttatct gcgaaacgct cgatctcgat
    17881 cttcggaaca cctgctgcat acaggttctt cttgatgaac ttacgaacgt tataatcttc
    17941 taccagcgta tcgccgaagt cagacttatt cgcaaaccag cgggaatccc aatctttaat
    18001 gacgccgaca cggagaccgt gcggattaac cttctggccc ataactcaac ctcctactgg
    18061 attttactct tcttctttca gaacaatcgt gatatgtgaa gttctcttca aaatgcgata
    18121 ggctctgccc tgtgctctcg gcatgatccg cttcatggtc ggacccggtg ttacgaagca
    18181 ctctgcaaca taaagattat ccttgttcat gctgtgattg ttttctgcat ttgcaattgc
    18241 ggacttcaaa agcttttcca gcagcgggct ggcagccttc ggagtgaggt ccagagttgc
    18301 cagtgcgata tcaacaggct tattacgaat cagatccaga acgatctgca ccttgcgggg
    18361 tgaaatgcgg gcatttctca gatatgctct tgcttccatg tgatctcctc cttccgctta
    18421 ctttccgtta ttggatgtct tagaaccagc gtgaccctta aaggttcttg tcggtgcgaa
    18481 ttcacccagt ttgtgtccta ccatatcttc tgttacatat accggaacgt gcttgcgacc
    18541 gtcatgcaca gcgatagtgt ggcctacgaa atccgggaaa atggtagaag aacggctcca
    18601 tgtcttgagc actttcttct cgccggcttc gttcattgcc tgtacacgct tgaggaggac
    18661 ttcctggacg taaggtccct ttttgatact tctgctcatg agtgtgttcc tcctttccct
    18721 tatctaccgt tgcggcgctt tacaatatac ttatcggatg ccttgtgctt tgcacgagtc
    18781 ttataaccca gagccggctt accccaagga gtaacaggtc ccggacgacc gatcggagac
    18841 ttaccttcac caccaccgtg cgggtgatcg ttcgggttca taacagaacc tctgactgtc
    18901 ggtctccagc ccttgtttct catgcggcca gccttaccgt aatgtacgtt ttcgtggtcg
    18961 atgttagaaa cctggccgat agaagcacgg cactcgatcg gaacttttct catttcgccg
    19021 gaaggcagac gaaccagtgc gaaagcacct tccttgccca tcagctgagc catgttgccg
    19081 gcgctgcgta ccagctgtgc gcccttgccc ggatacagct caattgcgtg gataaaggta
    19141 ccaaccggga tgtcgatcag tttcagtgcg ttacccggct tgatgtctgc atcagagcca
    19201 gagcgaacta cgtcgccgac cttcaggcct tcagctgcca ggatgtagcg cttttcgcca
    19261 tcctcatact gaaccagtgc gatgaatgcg cttctgttcg gatcgtattc cagagtcaga
    19321 acagttgcgt tcacgccgtc cttgctgcgc ttgaagtcga tgacacgata ttttctgcgg
    19381 ttaccgccgc ctctgtgacg aacggtgatt ctgccgtagc tgttgcggcc cgagttcttc
    19441 ttcagcggct cgagcaggct cttctccggc tttaccttcg acagacccga ataatcggtc
    19501 acagtcatat ttctgcgaga cggggtcgtc ggtttatagg tcttaatggc cattcttttc
    19561 actcctcata aaatgccaga tttgcgggag catcactccc atacttgtta cagcctgtta
    19621 attatctgta acttagaaca taccgtcgaa gaattcgatg gtacccttgc gcttcttgcc
    19681 gtccttgtct gcggtcggct ccggattgat ggtgataaca gccttcttga aatccgggcg
    19741 cttgcctacg cttctgccca tgcgcttggt gtgaccggtg cagtttacgg tgttaacctt
    19801 cagaacctgt acctgaaaca gttcttctgc tgcctttgca atttcaacct tgttggcatc
    19861 ctttgctact ttgaaagtgt acttgctctc agccatatcg tcgacgctgc gctcggtgat
    19921 gaccggagtc agaatgatat cctgtgctgc tttcattttg cgtacacctc ctcgatcttt
    19981 gcgacagcat cctctgcaac gatgaacttg tcatagttga ggatgtcata tacattcagg
    20041 gtgttgacag ctgccgtctt cacgcctgcg atatttgctg cactcttaac gaccttctgg
    20101 tcagcctcag cggtaaccag cagagccttg ccctctacgt tcagtgcctt cagcattgca
    20161 acgatggtct ttgtcttgaa ttcttccacc ttcagggagt tcagaacgat catgttctga
    20221 tccagaacct ttgtggagag tgcagacttc atagcaactc ttcttgtctt cttggtcaga
    20281 gcgtaacgat agtcacgggg cttcgggccc agtgcaacgc cgccgtgtac ccactgcgga
    20341 gcacggatag aaccctgtct tgcgtgacct gttcccttct gtctccacgg ctttctgccg
    20401 ccgccggcaa cctcagtgcg ggtcagtgta gactgtgtgc cctgacgctg atttgccaga
    20461 taatttacga ccattgcgtg catcactgcc tcgttaggag tcactgcgaa aactgcatcc
    20521 gaaagttctc tctcagacac ctgctggcca gccatatcaa atactgcaat ctttgccatt
    20581 gatgtttcct ccttccttac gctttcacgc tgtcagtgat gacaacaatt ccaccctttg
    20641 cgcccgggac agcacccttg atggcgatca ggttattttc actgtcaacc tttacgattt
    20701 ccagattctg tatggtaacc ttttcagcgc ccatgtgacc tgccataccc ttgcccttgt
    20761 agatacggga cggggaggag caggcaccca tagaaccagg ctggcggcct accggaccag
    20821 taccgtgagt ttctctcagt gtgcgctgat tgtgacgctt gattgcgccg gcatagccct
    20881 tacctttgct ggtgccgctg acatctacgc tgtcgcctac tgcgaaagtg tctgccttta
    20941 cgacatcgcc tacgttcaga gctgcgcagt cagccagacg gaattccttc agtgtcttct
    21001 tggctgcgac atctgccttt gcgaagtgtc cctgcatcgg cttggtaacg tgctttgtgg
    21061 tcacatcgcc atagcccagc tgaactgcat catagccgtc gttttcggtt gtcttcttct
    21121 ggatgacaac gcacgggcca gcttctacaa cagtaaccgg gatcactttt ccggcttcat
    21181 cgaagatctg ggtcatgccg accttcttgc cgataattgc tttctgcatt ttgatttcct
    21241 cctgttggtt attggttgac gattcagatc cgtcaacatg accggcggta tgcctaccgc
    21301 cctgcggcaa tttctgccgc tttcagaatc agagattctt acttgagttc cattacgatg
    21361 tctacgcctg ccgggatctc cagcttctgc agagcctcag tggtcttctg cgtcggacgt
    21421 acaacgtcga tcaagcgctt gtgagtacgg cgctcgaact gctcacggct atccttgtac
    21481 ttgtgagttg cacgcaggat ggtgacgatc tccttatcgg tcgggagcgg gatcggaccc
    21541 gaaacctgag aaccgcttct ctttgctgcc tctacgatct tggctgctgc tgcatctact
    21601 gtagcgtgct catagccctt gatcttgatt ctgatttttt cgttgactgc catttttttc
    21661 gcctccttcg tattgttggg gggccggaga atttctgctc tccgtcagga agttttccac
    21721 gccgccgggt gtttccagcg ccggcgtaca aaaaagctcg aaaacgcaaa tgtttccgag
    21781 ccagtgcaaa cagcagtaca aaccggcata gctagagcca tgctggtgaa atgtacacag
    21841 actgtgtggt tatcctgatt cgcccggttt taaatcggac atactccacg aaaattacct
    21901 gacataccgc cagcaacctt tcgcttcatc gcatatctat tgcaatcacg cttgtatatt
    21961 atagcatctt tccggaagga atgcaagcct tttgccgatt tttctttcga ttattttgta
    22021 aactttttgt aaagccgcaa aattcacgct tttaccggtc tcctgcacgc cgaaatccct
    22081 atataaatcg tat
//
LOCUS       DAWENO010000016        22062 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_984_length_22062_cov_12.998273, whole genome shotgun sequence.
ACCESSION   DAWENO010000016 DAWENO010000000
VERSION     DAWENO010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 22062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22062
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_984_length_22062_cov_12.998273"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2274..2283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9364..9373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5447 a         5987 c         5652 g         4956 t
ORIGIN      
        1 cagatgaagc cattgcatat ccgtcagaca cgatgtcaaa acaggacata aaaaggggaa
       61 ttgggggatt tcttcaatcc atgcttttag tataccggaa aaagcttaaa ataggctgaa
      121 agaaaccaca ctttctgcaa atttttccat agctatgcaa gagacgcaaa aaagcctgca
      181 cgccatccgg cgcacaggct tttcatttta tttcgattta cgcaaaaggc accaatcagt
      241 taatgatggt ctcctcagtt tccttgtcga acaggtgaat cttgttcgga tccagagcga
      301 tcttgatctc atcctgcgga cgagcggtag agcgcggatc aacacgagca gtcatctgaa
      361 cgccagcgca taccaggtac agataagtct cagcacccat catttcggtg atttcaacag
      421 tagcgtcaac aacaccggta gatgcgttgg acaggaacat ctcgtcatcg tggagagcct
      481 ccggacggat acccagagta acttccttgt caacatactt tgccagctcc ggagttacct
      541 ttgcagccgg aacttcaacc tgatacttca cagccttgct ggtcttggta gcatctgcgc
      601 cgaactctac atagtagttg tctgccttct tcagaacagc gtcgatgaag ttcatctgcg
      661 gagatcccag gaatcctgca acgaacttgt tgcccggacg ctcgtacaga gcctgcggtg
      721 tatcgatctg ctgaatgata ccatccttca taactacgat acggtcaccc atggtcatag
      781 cctcagtctg gtcatgtgtt acatagataa atgtggtacc cagcttcttg tgcagcttgg
      841 agatctcagt tctcatctgc gcacggagct ttgcgtccag gttggacagc ggctcgtcga
      901 ggaggaatac ctgcggatta cgaacgattg cacgacccag tgcaacacgc tgtctctgac
      961 caccggacat agccttcggc ttacgatcca gcaggtggct cagatccagg atctttgcag
     1021 cctcttctac cttgcgtgcg atctcgtcct tcggtacctt acgcagcttc agaccgaatg
     1081 ccatgttgtc gaatacggtc atgtgcggat acagagcgta gttctggaat accattgcga
     1141 tatctctgtc cttcggagcg atgtcgttta ccaggcggtc gccgatgtac agctcacctt
     1201 cggaaatttc ttccagacca gcgatcatac gcagggtcgt agacttaccg catccagacg
     1261 gtcctaccag aacgatgaat tccttatcct tgatttccag gttaacgtcc tttacagcga
     1321 ctacattgcc cggatacttt ttataaatat gtcgaagtga taaacttgcc atttgcgcag
     1381 ttcctcctaa ataagatttt tccagaacgt cctcacgggc gtttcccgtc cgccgttctc
     1441 gatgatatat atatcatacc aaaaataaaa acatttttca agtcgttatt tgcccaaaat
     1501 tctgcttgta catttatcca ttatgacgaa tgctttttta gcgtttgtag caaacttgca
     1561 aaattggttt cacaattcag cattttttgg acatcttttt cagacagcac acagcacgtg
     1621 ccaatttcca gatcctgggg catttccagt ccgcccatgg cgatcctcgc cagctctgcc
     1681 acatgatttc cagccgccgc aaacatccgc cgcacctggt gatattttcc ctcgtgcaga
     1741 cagatcagcg cctcccggtc tgtgccttcc accggggcag cctttgccgg cagacactgt
     1801 gcgccatccg ccagcaccat gccctctgcc atcttttccg catagccgtc ctcccagggg
     1861 cgagccagca cgatgtgata gtatttcgcc acgtggctcc gtgccgcaat gacctggtgc
     1921 gccagctgcc cgtcatcggt gagcagcagc atcccggtag agtctttgtc cagccgtccc
     1981 acaggtcgca ggtccttccg gcacagctcc ggcggcacaa gttccagaac gctcttctgc
     2041 cccggctcat ccgcagagct gacatagccc ttgggcttgt gcagcagcac atagagatac
     2101 tggtcgccgg ggatgtgtct gccgttcaga cagacagtgt tctcgtccgg ctgtacgtgc
     2161 tggtctgcct ttcgcaccac agcgccgtcc acggtcgcct ttcccttctg gatcatctgc
     2221 cgcagttccc ggcgggaata catggtgcgt gcagagagaa actgatctaa tcgnnnnnnn
     2281 nnnctaatcg catcagagac ctcctgttct atttttcgtt tgtccacata gattataccc
     2341 agagaaacac acagcatttt cgaaaggcgg tctgcatcat ggaaaaacga gggattctgt
     2401 ttcttggcgc cgcaggcgtg atcttcggct gcggcttttt gtggcactgg ctgcatctgc
     2461 cggcaagcgc accggagacg caccagatct gcctccagga cggcgcacag cggctggcat
     2521 tcctggagag ccagggacag ccggagcgct gctgcgtcgc cacagagact gttcagctgc
     2581 ctgctgcggc ggacggcagc tacagcgagt acgccgcact ccagcaggca caggcgctgc
     2641 cgctggcatc acacctgggc gagacagccg tccgctatac ttatacacag agcagccccg
     2701 gcgaggcaca cctgcgcaca gagctgctgc tgagtgagga tcagatcctg ctgggtgcgg
     2761 tgcagtatga ctgcacccaa ccggaacaga tgtctccggt catgcagccg tagactcaga
     2821 actcacttat caaacacgtg gaacagtccg ccgatcagcg gcagctgctt tgccttgccg
     2881 ttcgctgcat tgataatacc aataatcatc agcgccaggc ttgccagaga cagcaatgcg
     2941 gagagaactg cgcccaaaac cggaatcttt cccagaatgg cgctggcgat gcccacaaca
     3001 atgttcgcca ggaacaggat aaatccctgg tttgcgtgat atttggcata gcgggactgt
     3061 ccgccgttta ccagcagcgg caggataaac aggatgccca cataggacag cgctgcccag
     3121 accttgttgg tctggatatc ctgggtgtca tacattccgg tatcgtccgg ggtattcata
     3181 aaatcattga aatcgttgct catggtctga tcttcctttc tgctctgcgc tgccgtaaat
     3241 cctcttcatc cagtgcgccg atggcagcta aaaatccggc tacatctgta aaatcctgat
     3301 agaccgaggc gaaccgcaca taggcgaccg ggtcgatcag tttcaaacgt tccatgaccg
     3361 cattcccgat ctccgaggag gtgacgacgc tgcgcatctc ctgggcatac tgggattcca
     3421 gctcgctcac cagtttcagg atgaggctgg aggtcacggg acgttttttg atggcattga
     3481 aaattcctgt caccagttta tgacgatcaa acggctcata agaaccatct tttttctgta
     3541 ccatcagcat ggggcgttcc accgcctcat aggtcgtgaa gcgctttccg caccccaggc
     3601 attcccggcg gcggcgaatc ttttcatccg tcgaacggga gtccatcaca cgagtgttga
     3661 tgtttccgca aaagggacaa tacatagcgg cattacacct tacctttccc tgccgtctct
     3721 gcggagcgaa tccgattctg gagcagcgtg taattggcgg caagctctgc cacatcggaa
     3781 aagccgctgc ggtagagttc caggatcacg gagcagtccg ggctgacctg cgccagcatt
     3841 tccagaaaag cgctgatccg aaagcgcccc ttccccagca gaaggcagtc gccgtattcg
     3901 ccgtggtcgc tcatgtggac gtgtaccaca tgagacccca gccctcgcag catggcaagg
     3961 ggatcttcgc cggagcgcac cgcctgcttg atgtccagga cgaatctcgc ctgtttcccc
     4021 agggcatccc gcatttccag cagaaacggc agggatgcgc tggtacagcg tgccacgttt
     4081 tcctgcgcca cctggacgcc gtaggagtct cccagctctg ccagccggcg aaagcgttcg
     4141 aaatacatct ccttgttgac cgatgccgcc gggactttgt tcccgtgcag cacgaacacc
     4201 tttgcgccca gctgctgcat ggcggaaaaa taatgccggc agtattccag atagtcgtcc
     4261 gcacgccgtg gataattgga aaagagcatg gtcggctcaa tctcacaggt gaacgggtgc
     4321 agcgagacac aggtcatgtc gaaccgatgc agcaggcgtg ccatggtatc cacaaagctg
     4381 cggcgcagtt cagagtggct gttgatgaaa atttccacat tagacacgcc ggaaagcgcc
     4441 agatcataca gtgcctcttc caccacccgt ggatacaggc aagccgtaga aacgccggca
     4501 cgcagcatac aaccactcct ttctgcctgc attctgccct gcaccggaaa tggtgcggca
     4561 ggcttttttc attataccat acccgaatcg aaatttcaat agatttttgt acagatgtac
     4621 gaaaatcgat tttcatgccg cagatgcgca aaaaaccggc acgggcgctc ttgcccatac
     4681 cggttttctg ttcctatatc aagcgaaaca aacgcttata cgcttacctt tgctgccttt
     4741 ttcttcggcg gtttcagcac gctgatgcca cgttttttct gccaccatac ccacaggctc
     4801 ggcacaaagc agatggagtt gaacgtaccc attgcaatac cagccatcat cggcacggag
     4861 aaactcagaa tggaatccag acccattacc agcgcaacga tggtaacgat cagcatggag
     4921 ctgaatgtgg taacagaagt acggatactg cgtcgcagcg actgggagtc actgatgttg
     4981 accagttcct ggatctgtgt gcccggcagc agtgtctggt tctcacggat acggtcgtaa
     5041 ataacgatgg tatcgttaac cgcataaccc agcagagtca ggataactgc cataaagttg
     5101 gagtcgatct caaaccggca cagaaccacg cagccatagg tgacgatcag tgtggagagc
     5161 agtcccagca cagcgcacac acctgcggac cagccgctga tctgcttgaa ccggagcgca
     5221 atgtacacga tcaggatcag tgcagcgaat actgctgcca cgatgcactt ggcgaagaat
     5281 tcgccgccgg aacgtgcgtt aacgtcgttg gcgtccagca gctctacctc gttgtccggg
     5341 cagagttcct tcaccttgtt ggtcacttcg ttctgacggt cgatggtcag accttcggtg
     5401 gaagtgaagg acagggacag ggtcttggtg tcgctgtcca ggctgtcgcc ctgctgtacc
     5461 tttacggtag agccgaccaa actggtcagt tccttggaaa tgctgctttc gtccacatca
     5521 ccggtatagc tgtaggtgat gatcgtaccg cccttgaatt ccagcgcaac gtctacgccg
     5581 gtaaaggtgg agagcaacga gatcaggatc agtgcaaagc cgatgataaa gaacagcttc
     5641 gacttggaac agaaatcacg tacttttacg tctacatcaa aatgctttgc gtccttagtc
     5701 tgctttgaca ttgataccac ctccatagag tttcttgttc tggaagcact tgaaccgaga
     5761 cagagacatg gtcatcagtc ttgccgcaaa cacgcccatg ataaagttca taatcagacc
     5821 ggtcatcagt gtaaagccga aggagtagat aacgccctct gtggtcgcac cgaagaacca
     5881 gaagatcgtg tggttgaaga tctttgccca gatgctgtct ggcacgccga atgcacccat
     5941 cagcacgatg gataccagga taccggtcag gttaccgtcc aggatcgcac taaacgcacg
     6001 gtgatagccg gaatacagtg ccgtatccag cgtcttgccc ttgcgcagtt cctcacggat
     6061 acgctcgccg gtgatgatgt tcgcatcgac gcccatgccc acagacagga tgatacctgc
     6121 aataccaggg atcgtcagcg tggtgctgga cataaagccg aaccagccgg aaacagccgc
     6181 cagcataccg gcaacctgtc caatcagcgt gatggatgcc accacgccca gcaggcggta
     6241 cagggcgatc atgtaaatca cgatcagcgc cagggtgata acgccggcaa tcaccattgc
     6301 cgtcagcgca ccctcgccca tggtcggaga aatggtctta aagcttgtcg tctccagaga
     6361 gaacggcagc gcaccggaat tgatcttgtc tgccagttcc tttgcacttt catatgtaaa
     6421 gttaccggaa atggatgcct tgccgtccga gatcactgcg ttgacagacg gtgcagagac
     6481 catggtgttg tccatccaga tggagatctg tcccttggac tgatacagct ccgaggttgc
     6541 ctcatagaac ttttctgtac cgctgtcgtt cagatccagg gtaaccaccc aggtgatgtc
     6601 tccggtggtg tcatccttgg aaccgccggt agatgcctgt gccacgtccg caccggtcag
     6661 cacgatatcg cccgacggaa cggtgtcgcc gttttcatcg gtggtggtgt ctgtaccctt
     6721 gcggaaggtc agttctgcgg tctcgcccag ttccttgaca gcctgttccg gattgaagtc
     6781 cttttcgcct gcctgccacg gaaaacgcac gataatacgg ttgctcttgt agtccagata
     6841 cgtttcgctg tcgctgatgt tctggtttgc cagacgcagc ttgatggtct ccagcgctgc
     6901 atccatttgt tcctccgttg catccacatc gcccgccggt tcaaaggtca cgtctacgcc
     6961 gccttggatg tcgatgccga gacggatatc atccaccccg tgaacatagg tctttaccac
     7021 atcaccaaac tgggtatgca cgccgaaaat ggtggtgagc gtaaacagag cgattacggc
     7081 aaatacaatg aagaaaacgg gttttttcac ttttctcacg atttagcctc ctgtgctgcg
     7141 gtctgtctgt ctcaggttcc gcataataaa ctgtgccgtc ggcagaatgc acgatcgaca
     7201 attacttatt attatattcc aattttcgca attagtcaat aggcacggca aaacttgtac
     7261 aacattgccg atttgaaagc gcttttttcg gcgacttctc caaataccgg gagaaaactt
     7321 ttggaattgc cgttgcaatt ccacgatact tatgatataa tcatatcaat agaaaaatac
     7381 gattccggcg ccggcacagg cgctccgaaa ggactgacag acaatggtac acttcggcaa
     7441 cgactgggac accatcctgg acggcgaatt tgccaaaacc tactatcaaa atctgcggca
     7501 gtttctgctg acggaatacc gcacccagac cgtctacccc ggtatgtacg acattttcaa
     7561 cgcactgaaa tacactcctt acgccaaagt gcgtgccgtg atcctgggac aagaccccta
     7621 tcacggtccg aaccaggcgc acggactgag cttttccgtg cggaaaggcg tagcgccgcc
     7681 gccgtctctg gtgaacattt tcaaggagat caaggatgat gtgggcatcg acaacaccgg
     7741 caagcacggc gaactgacca cctgggcaaa gcacggggta ctgctgctga acacggtgct
     7801 gaccgtccgt gccggacagg cgaattccca caagggcaag ggctgggagc agttcaccga
     7861 tgatgtgatc cggctgctgg atcagcggga agaaccggtg gtgttcctgc tctggggcaa
     7921 caatgccaag gcgaaaaagg ctctgatcca gcacccccag catctggtgc tgactgccgc
     7981 acaccccagc cctctgtccg cctaccatgg cttcttcggc tgcaaacact tctccaaggc
     8041 aaacgctttc ctccgggaac acggtctgcc ggagattgac tggagcatct gacatggcat
     8101 attcctatcg ctggcagtac tgcgacatgg attttttgcg gcactgtctg aaaaaataca
     8161 gcctgctgct ggagaaaatc ccatcggcga ttctcacgga ggaaaccacg ggaacgcaga
     8221 tgttcgtcac ggaatttctc tccgactgca tcaaacggtg tgatgacgga ttcgacgatt
     8281 acaccatgac ggcacttttg ccggaagggg cagccactgt cacgctgcaa gaaaacaggg
     8341 atacagttcg ccgtctttcc acccgctatc acggcgaata tgcggcactg ctgccggaaa
     8401 tggcagccta ttacttttcc aaagccggca gacagcagcg gcgggatacg gaacaggctc
     8461 tgggaaaacg ctgtttcgca tttttcgatt atgcgtacaa cagaatgttg gaagcgcagt
     8521 ttctccggca cgattttcgc tgggtgggcg gcgaaattcc ttctgtcaac catttgaaaa
     8581 cgctctccga cgctgccgat ctgaccgagg cactggggca attccgacgg gaagaatatg
     8641 acagacgaaa ggaacacgat ttctcccatg caaagctttg cactttcctg gaaacccggt
     8701 ttgggatgct gtattttgaa aatcggtttg ctgcgtacct gctgcagccg gtttccggcg
     8761 gtttttccgc agatgacggc agcacggaaa gctggctgct gtggagatgc agtcggctgc
     8821 tgggcggcat tgccctcacg gcagagcgaa aagtgtgccg cctgtttgga actgatgaaa
     8881 acgaacggca gcaggtgctg gcggcatact tttgtttctg ccggcaaaag gggatcccct
     8941 tttccggaga gatacagctg tgccagccgg aatatgccga gccgctggga aaggattttc
     9001 tgccgtggaa gtgggagtcc ggcaaggtca cgccacagct gctgcaagcc atagccgacc
     9061 aactgcggca atgtgtcccg gaacaagaca cagactggtg ctggctgctg ggaacacggc
     9121 tggtattcac cgtgctgccc ctggcaaaag aggcattggc agcactgccc cagccggaac
     9181 gggatgccct tttcgtacag gcggaacagt tcctgcgaaa aacggcagtt gctttaggtg
     9241 accagccgca gccgctgaca gaaacggact ttgaaaagct gacggcacaa tatgacaagc
     9301 ttttgctggc agattcggcg gaaaagttac agcgatattc ccggctgtat cccacatcag
     9361 cacnnnnnnn nnngcacacg ccaagtttcc gatacagcag aaagacggct atgtctggcg
     9421 gtcggaacga ccagtagagc aatcggcaaa aaatgctcca gacggcacca ctctgccggc
     9481 agtccgcagg gaattgttgt tggaacagct gcaattgccg ccggaaaaga tcgccctgga
     9541 acagatctat cccctttgct atgaagatat ggctggtttt tccgaaaacg gaagatactg
     9601 caaacgtggc gaagattttc tgaaagaagc accgcagctg ctgcggaatt atgaaacaca
     9661 gcacgctgcg gttctccggg agcggtactc gccggaggaa tggaactggt tccggcagag
     9721 cgccgatcag gtataaaaaa aaatgatatc aagcgatacg atttttccgt ctacccaaac
     9781 acctacaaat atgctataat atgattgtac tactaaaata tcatatattc cggcacacgc
     9841 cgcagaaagg aaatagatat gaaaatcgca tccctgttct ccgaaaagac tgttttttcg
     9901 tttgagatct ttccgccgaa aaagacttct tccatcgaaa ccatttatga caccctggag
     9961 gcgctccagg atctgcaccc ggattttatc agcgtgacct tcggtgcagg cggcagcggc
    10021 aacggcgaaa acacccttgc tattgccaag cggctgaagg aacactacgg cgtactgccc
    10081 atggcgcatc tgccctgcat ccactattcc aaggcacaga tcgacaccat cctggagcag
    10141 ttccgggaaa acggcatcga aaacatcctg gcgctccgtg gcgaccgggt ggagggctta
    10201 gaaccgacca ccgatttcga ccacgccgcc gaccttatcc gctacatccg cagcaaggac
    10261 ggcagctttg acattgccgg cgcctgctat ccggagtgcc acccggaggc gcccaacatg
    10321 gatacggaca tccgccatct gaaggaaaag gtggatgccg gcgcaagcca tctcatcacc
    10381 cagctgtttt tcgacaatga ttcgttctac gacttccgct atcgtgctgc ccgtgccggc
    10441 gtgaatgttc ccattgaggc tggcattatg ccggtgacca accggaaaca gatcgagcgc
    10501 atggtcacca tgtgcggcgc aagtctgccc cggaagttta ccaagatggt acagcgtttc
    10561 gaggacaatc cggatgcgct ccgggatgcc ggcattgcct atgccatcga ccagatcgtc
    10621 gacctggtga ccgacggggt ggacggcatt cacctgtaca ccatgaataa tccctatgtg
    10681 gcacggaaaa tcagcgaggc agtcggcggc atcattaagg cagcgccgaa atgctgaccc
    10741 tgccgctgaa cgagatcgac aagcaggagg cgctccgcta tatgggatgt cccggtgcgc
    10801 cggatgcgtc cctgctggca gtcgtcagcg actgcgaaga aaagctgcga cgtgctgttc
    10861 tgccgggcgt ggtctaccgg gtgctgccgc tctgccggga aaacggcgtt ctctctgccg
    10921 gcggcatggt gcttgccgga gaagatatcg cacggcatct ggcagagtgc gaccggatca
    10981 ttctgatggc ggcgaccctt tccggacaag cagaccagct catccaccgt gtgggcatct
    11041 ccgatatgac acgctccctc gccatggatg ccctggcaag cgccggcatc gaacaggtct
    11101 gcaaccgggc ggaggaatgc tttcgggaac agctgccgga ggtctacttt acctggcggt
    11161 tcagtccggg ctacggagac ctgcccctgg cgctccagcc ggaaattttg cagctgctgg
    11221 atgcacaaaa gcgtctgggg ctgacggtga cgccggagca tatcctcatc ccccggaagt
    11281 ccgtgacggc gatcatcggc ctggcggatc atccgctgaa aaagggcgca cggggctgcg
    11341 ccacctgccg gatgcgggaa acctgtatgt tccgcaaagg cggcacacat tgctaaaatt
    11401 gcgaaaacaa cttgcagcat ggacgcagat acctgtgtcc atattgtgtt atctggaact
    11461 tgttttccta agacacacct tttcggcgaa tcctacggaa acacgtacta tattaaaaag
    11521 gagtttttct tatggaatat gaaattatgc cgtatacaga ggctgacgta gatgctatga
    11581 tcgagatctg gaacgaagtt gttcgggacg gcgctgcatt cccccagctg gacgaactga
    11641 accacaagac cggcgtggaa ttcttcggca gacaggattt tgccggcgtg gcaaaggtaa
    11701 acggcgaagt agccgggctg tacatcctgc accccaacaa cgtgggcaga tgcggtcacc
    11761 acgccaacgc cagctatgcc gtttcttcga aaatgcgtgg catgaagatc ggcgagggac
    11821 tggtgcgcca ctcgctggaa aaggcaaagg agctgggcta ccgcattctg atcttcaatg
    11881 ccgttgtcaa gggcaatgac cgtgcgatcc agctgtacac caagctggga ttccagcgtg
    11941 tgggcgtgat ccccggcggc tatctgctga aaaacggcgt gtaccaggat atttatgtgt
    12001 attatcatgt gctgtaaaaa ctattgaatc actttttgga aaggacactc ccctatgctt
    12061 tcttccctat ttcctcaccg caaattcatt cgtctggacg gcggcatggg ttccatgctg
    12121 caaaaaggcg gtatgccggt cggcgcactg ccggaagtct acaacatcac agaaccggaa
    12181 cgcatcattg atgcacaaaa acagttcgtc gccgtgggtt cggacatcat ctatgccaat
    12241 accttcgggg caaaccggca caaaatggca aaatccggct attctgtcca ggaactgatc
    12301 gccgccggca tcgcctgcgc ccggaaagct tgcgccggaa cggagaccaa ggtggcgctg
    12361 gacatcggcc cgatcggaca gctgctggaa ccgctgggaa cgctgacctt tgaagaggca
    12421 tacgatattt tcaaggaaga ggttctcgcc ggacaggatg ccgacctcat cgtcattgag
    12481 accatgaccg acctgtacga gaccaaggcg gcactgctgg cggcaaagga aaattccgac
    12541 aagccggtga tctgcaccat gacctttgag ccgaacctcc gcacctttac cggctgcacc
    12601 gttgcctctg cggcaatgac gctggaaggt ctgggagctg acgctgtggg cgtgaactgc
    12661 tctctcggtc cggacgaact ctttcccatt gtggaggaga tggcaaagtg gaccacgctg
    12721 ccgctggtgg taaagcccaa cgccggactc ccggacccgg tcaccggaga atatctggta
    12781 gacccggcgg aatttgcaca ggctgtggtg aagtttgcac agctgggcgt caccgtctac
    12841 ggcggctgct gcggcacgac accggaacac atcaaggcgg cttatgacct gctggaacag
    12901 gcagagatcc cgaaacggga aaacatccag atcccggcag cagtctgctc tgcatccgag
    12961 gtggtgcaga tcgaccagcc ccgcatcatc ggcgaacgca tcaaccccac cggcaaaaaa
    13021 cggttcaagg cggcgctcca ggcaaacgac atggactaca tcctggatca ggcagtgcag
    13081 caggtggatg ccggggctga ccttttggat gtgaatgtag gtatgccggg catcgacgaa
    13141 aagggcatga tgatccgcac ggtaaaggcg ctccagtcca tcagcggcac acctttgcag
    13201 ctggactcca ccatcccggc tgtcatggag gctgcacttc gggtatataa tggtaagccc
    13261 atcgtcaatt ccgtcaacgg cgaagaggca tcgctgcaaa acatcctgcc gctggtcaag
    13321 aaatacggcg ctgcggtcgt gggactcacc ctggacgaaa acggcattcc caaaaaggcg
    13381 gcagaccggt tcgccatcgc caaacgcatc ctggatcgtg ccacggcact gggtatcccg
    13441 aaacgggacg tgtacatcga ctgtctgacg ctgacggcat cggcggaaca ggacggcgca
    13501 aaggagaccc tggaggcgct ccaccgggtc aagacggaac tgggactgaa aaccgtcctg
    13561 ggcgtttcca acatctcctt cggtctgccg aaccgtgagg ctgtcacacg gacgttcctc
    13621 accatggcgc tggaaaacgg actggatctg cccatcatca accccaatat cgcctctatg
    13681 gcagaggctg tccgggcgtt ccgcctgctc cggggattcg atgtaaactg tgccgctttc
    13741 gtggaggcat acgccaatat gccgactgcc acggcggcag ccacgccgac tgctgcggca
    13801 aaaccggcgg cggaaaaagc gccggagacc ctggatctgc cctacgccat gtcccacgga
    13861 ctgaaaaacg agggcgcaaa gatcacggcg cagctgctgg aaaccaccga cgccatggaa
    13921 gtggtcaatg acattctgat cccggcactg gacagcgccg gaaagctgtt tgaacagggc
    13981 aagatctttc tgccccagct gatccagacc gcaagcgtgg cgcaggcggc attcgaggtc
    14041 atcaagacca ccctcatcga acgcaatgcc gccccggtca gccgtggcga gatcgtcctg
    14101 gcaacggtaa agggcgacat tcacgacatc ggcaagaaca tcgtccgggt actgctggaa
    14161 aactacggct atacggtgct ggatctggga cgggatgttc cgtgccagct ggtggcagac
    14221 acggcaaagg aacacggcgt caagctggtg ggactttcgg cgctgatgac cacgaccctg
    14281 ggcgctatgg aagagaccat ccagctggta aaggcatcct gcccggactg caaaatcgtc
    14341 gtgggcggcg ctgtgctgac cgccgactat gcggaaaaga tcggcgccga ctattacgcc
    14401 aaggacgcca tggagaccgt agccgtcgca aaagaagtgg taggctgaca aggaggcaac
    14461 cattatgaca aacgagccaa acgagaccgg aacccgttct gatgcgccgg agagcgaaga
    14521 gaaaaaagtc cggaagtggg acttcgtcaa caagcagtgg gcggcgacca tcattttcag
    14581 cggcgttgtt ctgctgctgg tggggttcct gttcatcaag cacgagatct tcaccggact
    14641 gctgtcctat gtgtttgacg tcctgcgccc catcaccatc ggactggtca ttgcattcat
    14701 cctgtaccgc cccacctgtc agatcgacaa gctgtttcag cgtgtgcgga aaaagttccc
    14761 acgctttcct accggagcga tggcggtatt ctgttcctac tttctgatgc tcgcactgct
    14821 gtgcgtcatt atctggatca tcgtgccgca gtttatcacc agtatccggg acttcgggga
    14881 caacatcatg ctctactaca acaacgtcat gaagttcctc aacagcagcc ggggcgaaca
    14941 gatcctcaaa ttcctggagg acaacgacat caacccggcg atgctccgca ccaagctgat
    15001 gagtctgacc acctacatcc cggacgctgt gggaacgctc agcacctggg caagcggtct
    15061 catcggcggc gtgatcgact ttttcatcgg gctgatcttc tcggtctacg tcctcgccgc
    15121 acgcaacaag ctccgcagcc agggacggca gattctgcgg catttcctgc ccgaaaaaca
    15181 ctatcaccgg ctgtctcact acggcagact gaccttttcc accttctcca actttatcag
    15241 cggtcagctg tcggatgcgt tcattctggg cgtgctgatc ttctgtgtca tgagcatcgg
    15301 cgggctggaa taccccatga tgatcgccgt tatcatcggc atcaccaaca tgatccccta
    15361 tgtgggaccc tggatgggta cgattccctg tgcgctgatc ctgctgatgg tgaacccggg
    15421 tcatgccatc gcctttgtca ttatcgttat cattgcacag cagatcgaca gcaatctgat
    15481 ctatccccgt gtggtgggga cctcggtggg actcccggcg atctgggtgc tgttcgccat
    15541 cacggtgggc ggcggactgt tcggcgtcct gggaatgatc gtaggcgttc cggtcatgtc
    15601 catcatttat acggtgatgc gggaaaagac cgcctccggc acgccgaacc agcccagcca
    15661 gccggaaaag aaaaagcacc gctggcacat ccggcagtcg gtgcagaccc tttacagccg
    15721 ggcggcaaag cgaaaaaaca gcgaaaacga ccacagcgat tctgcgaagt gagcggtttc
    15781 ggagagagaa aaatgagcct gtttctgcgg aaacgggctt ttttgatttt gcaacgaaaa
    15841 ttcgacaaaa ttcgacaaag tatatctctt atttgaacag attcgacaaa acgaatttag
    15901 caataatgct gaaatttcgc tgttgatatt gacacacatg aacaatattg ctataatata
    15961 agcataagag aatcgcagaa aagcctaaat tttggaaagg agatgcaggc gtcagatgaa
    16021 atcgtttaca gatacgggac ataaaagagg aggaagaact atgaaaaaaa tcaaaaagat
    16081 attcgcagcc tgcatggcaa cactgattgc cggggcaacg ctggggagca tgagcgcact
    16141 gtcatcttcg gcggcaatat taggcgacgt gaataacgac ggaaaacttg atgtttccga
    16201 cgtagtaaca ttaaacaagt ttttagctgg tgaaggtgta cttagcaact acaatgttgc
    16261 agacacaaat gcaaacggaa ttatagacgg tgtggactct acgattttaa tggctttttt
    16321 agtgcatgaa atcaaccgac ttccgcatac aacaggctaa taaggaggaa atgtaatatg
    16381 aaaaagctga aaaaaatcct ggcagtctca accgctgtat ttgtattgtc tctttctttg
    16441 agtttgccta cagactgcgt ttctgcggca aattccaaca acgccgtttc atataccgtt
    16501 ttttcgttga aaagaggcgg aacctacaaa acaaagtaca cactaaatcc ggtggacacc
    16561 ttatcctcta caaatgcacg tgctgtcatt ggtgacgaca cacgagtggt tgacttttca
    16621 aaaagtggtg tggtaaaaat taattttact gctagtaatg gcgagtcata ttggggaacg
    16681 ggttttgtag ttgatgaaca caccattgct actgctgcgc attgtgtttg cgaacagaca
    16741 aatggcaaaa atgctactaa ttctggctca aaagttacag aaataaattt ctttaactct
    16801 gatggtacac caggattatc aattacagat gctgttgaag ttcatgttcc cacactctat
    16861 gtaaacggct cttactcaga ctataatcct tatgattatg ctttaattaa agtcaatgaa
    16921 gacttgagtg attatgcaat ttttaatctg ggcattatga atgataattt tatcaacaag
    16981 caaaccgctg tatctgttac tggattccct aaaaaagtca ataatcaaga agtgcataat
    17041 gtcatgtata cagggacagg ttttattcgc caaagcgatg ataatgaact gtactatgat
    17101 attgacacct ttggtggaaa cagcggcgga ccaatttata ttacttccac atttcaggga
    17161 aaagcatact atactgtaat cggaattcat gcttatggcg atgccaactc ttacaatggt
    17221 ggagccagaa taaccacaaa tttgctccaa ttctacaaaa acaaccccta tatctaaaga
    17281 aaggatgatt tttatgaaaa aacaattcca gagggttttt gctgcttttc tggcagctgc
    17341 tacaacatct gtttctattg ctggaatttc tgcaaatgca atgataggat atactagcat
    17401 tattccagaa aacgctgttg atattaccga tgtgtgtgaa tgtccaaaag gacaatattt
    17461 ggatgagtca tcctatatgt catactataa taatgacagc cacaccattg cctacttatc
    17521 tgacttcaag tttaattaca ctacacttta tgttacaaac aacgccgaat ttattaaatt
    17581 ctacaatgaa tacaacgcag atcaggtttt tgagaaatat gatgaagagg ttgttctgga
    17641 aactttttcc cagtacggcg agcgtctaaa agtcatgctt tatgacaaaa cagataagaa
    17701 cggcaatcac tccaccgatt tttccaacta taccaataaa cgagatgaag cgctgaaatt
    17761 ttgcagaata ttgcaggaaa aaggctatat cgctcatgca acttacacct ctttttctgc
    17821 aacaagtatg aagggcggat acgataatag cgtattgtta gaaaatgctg tgtcgggtaa
    17881 atcagaggaa atccgcaaga cagcagaggc ttttgactcc agcattgaag tgactgaaca
    17941 gtcctctgaa aacagcaatg tcagcacata taaaatcagc tccgtggaaa atattgagga
    18001 tggtgtagca atagcacggg aactcaagga taaattctgc aaaacacgaa acgataacga
    18061 agtttatcca tcctctgtaa ttgtcaccgg cgttcaagaa tctgcatttt ctagtagttt
    18121 ccaaggcagc gaaaccgatc tgacaacggc gcactacacc ggcgacatcg acaacaacga
    18181 aaccgttgac accagcgata tttttgatgt aatgtactac gttgcacgaa agggcgcagg
    18241 gatcgagaac gtttccttta ccgacggcaa cgcagtggaa gaggctgccg cctttgccgc
    18301 cgctgatatc gacggaaacg gcaagctgga ttccaccgat gtctactaca tgatgaagta
    18361 ctgtgcagaa aaaggcgccg gactgaaccc gagttgggga gattgaacga ttcgataact
    18421 atgtgaagtt gcaggggcaa tgtggaacgt tgcccctgct ttggtaaagc caagcgcttt
    18481 tgggtttaga aaggatgagt tttatgctga aaaaaataat tgcaggcatt gcagggctga
    18541 cctttgcttt ttctgtggta gtttcttccg gaacgctcca gacaaaagca atggaaacaa
    18601 ccacggaaac cactgcggaa gagacgactg acacagacgg cagcgaggtt cccatgacca
    18661 tacgtgttct ggatgaatcg ccgaatgatg aaatgtatct ttccggcgtt gcatttcaag
    18721 tcacgttgga cgagacagga gaaataatcc aacaatttgt gtatgacggc ggcgatttta
    18781 catttccact gcaatatgac aagacttata ccgtatgcgc aacctttgtt ccggaaggat
    18841 atcaacttga aaaacagggc ttttatgttg aagtctccgg atatgccagc atggactata
    18901 cattcagagc gaaaaatccc agtatcacta ccacatacac cacaacaacc gacccaaaca
    18961 tgacaacaga tgaatctttt accgaatcca ctacagctac aactgcaaca aaaaatcgga
    19021 aacttctccg gtacaaacaa ctgttaccac tgaaatggat cactaccgat ataccggcac
    19081 aaccggaacc agcagacctc ggctgacttc tggtacaacg gaaacgacag tctcaaagca
    19141 tgatgatacc gatatcatag aagttcccat gaccatacgt gtactggatg aatcaccaaa
    19201 tgatgaaatg taccttcctg gtgttgcatt tcaagtcata tcggaggaaa ccggagaggt
    19261 aatccatcaa tttgtatatg acggcggcga tttttccttt ttgctggagt atggaaagcc
    19321 ctatacaatt tatgcagtat ctttacccga aggatatcag ctgcaaaatg aaggcgttta
    19381 tgtaagatcc gcagaatacg agatggacta tactttcaga gcaaaaaatc caagtatcac
    19441 cacaacatat acgacaacaa ccgatcccaa tatgtcaaca gctgattcaa cagaaaccac
    19501 gtctgcaaaa tcttctgtga ctacagcaac aacaaaacca agaacaaccg aaacaactgc
    19561 acgcaccact ccggatgact atgaatatac cggaacatcc ggtacaacaa cgccggattg
    19621 gctgcattcg caaaccacca ctgaaagtac aacagccaac agcacgaccc cagattggat
    19681 gaattctcaa accactaccg aaaacacagc aaccaacagt acgactccgg atattgataa
    19741 aatcccctgc ggcgacatca atatggacag aagaattgac ctctgtgatg ctgttttgct
    19801 gaataaatat tgtgcaggag ttgtttcgtt caattctatt gcaaaagaaa aaagcgactg
    19861 taaccaagat ggcacggtgg atgccgcaga cagccttttg cttttgaaat ttttactgag
    19921 aatggtagaa aatctacctg gtaccagcac tgaaaccgta taaacaaagc atattcttcc
    19981 ctttctaacc ctaaaaatcc gcctgtccca aacggaacag gcggatttgc tgtacacaga
    20041 acccttattc actttcccgg tgcagttcct ccggctgaag ccgttccact tcccggagct
    20101 ggcgcacaat ggcacgggtg cgtgtccctg ccagaagttc cagctgctgc tctgcgccct
    20161 ggagactttt ctgcgccttt tcgatcgttc cggcgaactt ttcgaactcg ctgcggactg
    20221 cgcccagcac cacccacgcc tcgtggctgt gtttctggat ggcaagggtg cggaatccca
    20281 tttgcagtgc gttcagaatc gccgccatgg tggacggtcc ggcaacgctg atgctgtact
    20341 cccgctggag ctgttccacc agtccgctgt tcaccacctc ggcatacagc ccctcaaagg
    20401 gcaggaacag gatgccgaaa ttggtggtat acggcggctg aacatatttg tcccggatat
    20461 ccttggcgca gctgcgcacc acgctcagca gtttctgccg tgccgcctcc acctgtgccg
    20521 ggtcaccctg ttcccgtgcg tcctggagtg cggcgtaagt gtcccccgga aacttggagt
    20581 cgatgggcag ccacacaggc tgttcgccgt ccgccggcat ccgcaccgca aattccacgt
    20641 gattccggct gttgggcagg gttgggatct ccgtggcgta ctgctccggc gcaaggattt
    20701 cctgtaagat cgctcccagc tggacttcac ccaggatacc acgggtcttg acgttggaca
    20761 gcaccttttt caagtccccc acgccgaccg ccagcgtctg catttctccc agcccctggt
    20821 aaacttgttc caggcggctg ctgaccagct ggaaggactg cccgatcttc tggttcagcg
    20881 actgcgccag cttttcgttg accgtggatt gtatctccgc caggctgtcc tgctgctggg
    20941 tctgcatccg gtgaagattt gcggcaacgg cagagcgtat ctgctccatg ccctgggcat
    21001 tggaacgctc caggtttccc acacggcttt cgaactgccg cagccgtgcg tccatgccac
    21061 gttccatctg ttccaggcgc tctgcctggt agtccccgga ctggcgctgg ttttccgaca
    21121 gcagattgcc cagcatctgc atttggttct gggtgtcggc ggcgatctcc tgccgcagag
    21181 tgcgcagcgc tgtgtcatcc tgcggacggc gcagtttctg cacctgcatc accagccaga
    21241 caatggcggc gagctgtatc agacagagta tcagtaaaat atattgcatg gaaattcgac
    21301 ctcttttcgg gagtttttcg cagggacaaa cctctgcggc acattcggca aacggaaatc
    21361 aagaacgctc tctgcacatt ccgtccagag agcgttcggt tattctatca cgattctgtt
    21421 tcctgcttac cgttccagga tctggcttct gtccggaccg ttgcccacca taacgacctt
    21481 gcactcgcag acttcttcca gacgggcgat atacttcttg caagcctccg gcagttcgtc
    21541 aaagctcttg cagttggaga tatcttcgtc aaagccctcg aattcctcat agatcggagc
    21601 gcagtcagcc agggattcca acgcagccgg gaaatccttc agcacagtgc cgtccggttt
    21661 ctgatagccc acgcacattt tcagcgtgcc gatgccgctc agggtatcca gcttgttgat
    21721 tgccagaccg tccagaccgt tggtgcggac agagtgacgt acgatcacag catcgaacca
    21781 gccggtacga cggggtctgc cggtggtagt gccgaattca ccgcccttgt tgcggatcac
    21841 atcgccggtc tcgtcgttca gctcggtcgg gaatggaccc ttgcccacac gggtggtata
    21901 agcctttgca acaccgatga tgttggtaat catcttcgga cctacaccgg taccgatgca
    21961 cgcacctgct gcaatggggt gagaagaggt aacatacgga taagtaccga agtcgatgtc
    22021 cagcagggtt gcctgcgcac cctcaaacat aatggtcttt cc
//
LOCUS       DAWENO010000017        21493 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1025_length_21493_cov_11.053783, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000017 DAWENO010000000
VERSION     DAWENO010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 21493)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21493)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21493
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1025_length_21493_cov_11.053783"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4185..4194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4795 a         6512 c         5264 g         4912 t
ORIGIN      
        1 ggatacgctg tgtggtcagt tctttcagct gctgcatata cgggacagaa tcccggtaat
       61 tgcgccacat ctgaaaattg tccgcatact gttctgcgat ttggtgcagc gctgccagag
      121 cggcagcgtc cggcgcagca tcactgtgca ggtcatccag atcccacagg gtcacgccgt
      181 ctgtctcgcc gcaggtcggc tcaatgtctc tgggcattgc cagatccagc agaaaccgtg
      241 gctttcggga aagcgctttc agctgatcgc tgaaaatggt ataatgcgga ctgcgtgttg
      301 cactaatgac gatttccgag ttttccatct gtgcaaaacg ctgttcatag ggaatcggtt
      361 tcaccccgaa aggtatcact gtttccccgt ggtgatagga acgcagtgtc ataaacaccc
      421 ggcagccacg ctgtaccagc agtgatgccg ccaatcgtcc catgctgccg tttccgatca
      481 caaggcagct ttttccatcc agttttccga tgcactgttc cagctgctgc actgccccgt
      541 aagagacaga gagcggcacc tgtgagaccg acacctggtt ctgtgcgtcc tttccggcag
      601 aaactgccgt ccggaacagc gtgtccagca cactgtctgt ggtatggcat tctcttgcca
      661 gttccaccgc acgtcccacc tgcgttacga tctgttcctc atgcaggatc tgggaatgca
      721 gaccgcaggc aacctccatg aggtgctgcg ctgcgtccag ttctgttcgc accaggatgt
      781 gcggcggttc tgccgaagaa ccggcatagt gctgcaggag ctgcgccggg gaaatctgct
      841 ctgccggctg acgttctgct gcattgtttt ccgtccgttc ccggtcagac tgtacggaga
      901 gatacagttc tgttcggtta caggttgcca gcaatactgc gccgctgatg ccgtctttct
      961 gcaccagttc ctgcaagata tgccgcacct gctcttccga aaatgcaaag cgttctcttt
     1021 ctgaaacgtc tgcggaatgc acgtccacgc ctgccatata gatacacatt tcttttctca
     1081 gctcctttcc gtcagctgct gccgcaaccg cagtggttct gtaccagtca cagtacagag
     1141 gatcggttca cagtgtcctg cctgccacga aaagtacggc ttttgctgtt cttccagtgc
     1201 agaaagcaca gccatgatcg taccgccgtg taccactgcg gcaactgtct gatgctgctg
     1261 ccgctgcaaa gcctgttcca atgcctgaca gcatcgctgc aaaaatgccg ctctggattc
     1321 tccgctggga aaagccagcg ttccgccgct gtcgatccat gcctgatacg ccggatctcc
     1381 ggacagttct gcatagttct tcccttcaaa gtctccgaag tcgcactcct gcaaaccata
     1441 tactatttct ctcggcgtgt ccggaaaaag gatctccgcc gtctggacac agcgccgcat
     1501 gggactggtc agaaccactt ccggcatggg aaatgtctga cgaatttccg ccgttttctg
     1561 ctgtgccaga gaaatgcccg cctcacagag cggctcatct gtccggcagc cgatatagcg
     1621 ccgttccaga ttgccggcag tcatgctgtg ccggataaat acgatctgca tcacacgcct
     1681 ccgatccgca aacatcaaaa aacccgtgat tccagaagaa cacgggaaac agcatatggg
     1741 tacacaaagc cttgcctttg cagtatagcc gcaaagcgca ggaaaccgga tgcaccaaca
     1801 atttcttcta ttctcgtaga taggatttta ttccaaaaca gcagaccggt atcccggctt
     1861 cggttcaccg tattccatgc gccttcccgg aatcatagaa ttccagtggc atctgcatgg
     1921 cactccccga cacggtagcg gaactgtgca ggattctcac ctgcttccct gacatctgct
     1981 gccaattgtt cgtaaaacag agtccccgaa agaactcctt tgtcactgta cagcaccagg
     2041 atgatgccgt accattctac tataccatat tttctgtcaa ttggcaagcc tttttgcaaa
     2101 aaaagcagcc ctttccagga ctgctttaca aaaccgattc aatttatatg ttttgttcaa
     2161 atttaccggt acagctgata cagcaatgcg ttgcagatcg ccgccgccac attgctgccg
     2221 cctttcctgc cctttgccac aatatagggt acctgctcct gcgccagaat cagctccttt
     2281 gcctcaacca cattcacaaa tcccaccggc acgccgataa tcagtttcgg caaaagcttt
     2341 cctgcccgga tcagttcatc cagctgtatc agcgccgtgg gtgcgttccc caccgcaaag
     2401 atcagatcgc cgtccagact gcacgccttt tccatggaaa ccatcgcccg tgtcacgccg
     2461 cgtgctttcg ccgcagcagc aacatccggg tcggacataa agcagtgcgc ctggcatccc
     2521 agctgatgca gagagcgctt gttgatgccg gaaagcgcca tattggtatc ggtcacaata
     2581 tgcgccccgt tttgcagtgc ctttttcgcc gaagataccg catccctgga aaagcacaga
     2641 ttctccacat agtcaaaatc cgccgtggta tggatcaccc gcttgaggat gctccgctcc
     2701 ggctccggaa tctccacgcc cgtgagattc tcctcaatga tcgccatgct ccgggattcg
     2761 atctccatcg gcttatgata cagtgtctcc atctgtttct cctttccggt atctgtctaa
     2821 ggattccagc acctgtgaaa tggcgtggac ttcctcttcc ggcgcaccca gcagcactac
     2881 cggaatgtcc atttgttttg ccgcatccat tttttctgcc acaccgccac gcctgccgct
     2941 gtccttggag accagcaccg caatgtgata tttttccaac agttccagcg tatcctcttt
     3001 ggaaaacggc ggcattgcaa aaatgagatg cgctttggag tctgccgccg cccgccggga
     3061 atccggcgta tccaggattc tcgcccagcc acgggtgctg aaatccggaa tcgctttctc
     3121 ataaaaggaa agcgtattca ccccggtagt cagcaggata tttccgggaa ttgccgcaag
     3181 acaagccgct gcctgttcct tgctttccac ccggatgacc cgactgtaat cgtaggtcat
     3241 cttctgccgt cccagccgga aatacggcag ttccagttct gcgcagacct gccgcaccgt
     3301 ttccgtaacc acctgtgcaa agggatggga cgcatctatt accgcatcca tcccacgcag
     3361 tgcaaagcga aagccatccg catccagccg tcccacacgc accgtgcagt gctgcccttc
     3421 cagaatcgtt ttcccgtatt ccgttgctgc aaaggcggtg acgcagtcct gctccggcaa
     3481 gacggcaatg agatcggtcg cctcactcgt cccggcaatt accgcaagcc gcagcccttt
     3541 cacgcctgtg tctccgggtg gatgcagtag ccacggggtg taatcatttt cccgttctgc
     3601 acgtatgtct gggagttgcc gatcagcacc acacagaaca tatccaccgg ttcttccggc
     3661 agctttttca acgtggtgat ccacgccttt tcctcggcac gtccggcatt ccgcacaatg
     3721 cccaccggtg tgtccggcga gcggtgctgt ccgatcagca ccgccgcctg cgccagatac
     3781 tccgtccggg aacgggactt cggattgtac aggcagatca cgaaatctgc ctctgccgcc
     3841 agggacacac gccgccagat cagttccagc ggcgtcatgc agtcgctgag actgattacc
     3901 gcaaaatcgt gcatcagcgg tgcgcccagg actgccgccg ccgcacttgc cgccgtaacg
     3961 cccggcacgg caatgatttc cacatccgcc tgcatctccg ccgcaatttc cagaagtacg
     4021 cctgccatgc cataaatgcc gctgtcaccg ctggagacaa gcgccacggt tttcccggtc
     4081 agcgcagcct cgactgccgc ccggcagcgg tcggtctctt tccgcatggg cgtagcataa
     4141 tagcgcagtt ccgggaactg ctcctccagc agccggatat atgtnnnnnn nnnngcctgt
     4201 gtcacagcgt cgatcgcctg ctgtgtcata tacggacggt ctccgggtcc aaagcccaca
     4261 acataaatca aaggtttcat gaagtttccc ctttccatcg cagtgccggc agtttcttcc
     4321 ggcaggcggc aaatgtcacg ccgctgtatt tcacctttcc ggtcagcact tcgccgcagc
     4381 cggatgccag atacgccgat gcctccgaaa cagacggcaa ccccgtgacc tgctgcacaa
     4441 acggcgatgc ggcaaaggga taattgcatc ccagaatcgc atcatccaag acgatttcca
     4501 gcggaatccg gtatttctca cacagtgcca gaatcgccgg ctcgttttgt ttcagcgcaa
     4561 ctgtagcaat cttcgccaca gacagccggc tcagccgatg ctcttccaga aaccgcaaaa
     4621 agcactgttc cagatgctcc ggcgcaatgt tctttttgca tcccacaccc agcaccagac
     4681 ttttcggccg cagatacagc gtatgctctg cccggcagtc ccggagctgg tccgaaatca
     4741 ccacggcaca gggctgtaca ggatgggttt ccggcacgat attctccgaa aaggcatctg
     4801 gttctacggg cagatctgtc agaagtgtaa tcttttcctc tgccaccaga gcgctgctga
     4861 tgtgcttcag ctcgtgcaga ttctcgatca caaggtcatt ttgtttggca aacagatcga
     4921 acgccggaac acccgccaca tccgttgccg tggtaatgac agcctcaccg cccagatagg
     4981 cggcaaagcg ccgggcagcg gcattggcgc cgcccaggtg tccggacagc agactgatca
     5041 catattttcc ctgctggtcg agaaccagca ctgccgggtc gaatgctttc gactgcacca
     5101 gcggtgcaat cttccgcacg gcaatgcccg atgccataat gaataccagc aagtcacagg
     5161 cggcgaaatc acgccgcact gccgcatcga atgcagcctt gggttctact gtgccgccaa
     5221 atgctttttg cagccgcagc gccagctgtt ctccctgggg cgtcagccag aaatatgccg
     5281 ttttcatgcg tttttcccct gccggtactc gtgggtaaat gctttatcat acagcttgga
     5341 caatgcgtag ctgtccccca ggaaatcgcc tactgtcacc agcgccgtct tggtaatgcc
     5401 ggctgcctgc accttttcca caatatctgc cagcgttccg gtgacgatag tttcaaccgg
     5461 ccagctcgcc ttatagacca cagccaccgg cgttgtctcc ggataggcgg ttttcagccg
     5521 ttctgccagc tccgcaatcc gtcccacaga gaggaatatc accatggttg tgcgatgctt
     5581 tgccaactcc tcgatacgtt cccgttccgg cacaggcgtt ctgccttcca tccgtgtcag
     5641 tatcacagtc tgggtcacct ccggcagggt gtattctttc tgcatggcag ccgcagccgc
     5701 cagaaaagaa ctgacgcccg gtacaacttc atgagcgatt cccaactcgt ccagtgcatc
     5761 cagctgttcc cgtatcgccc cgtaattgga cgcttctccg gtatgaacac gcaccgttgt
     5821 gccgcctgcc tgctcggtct ctttcatgac cgcaagcacc tcttccagcg tcatcccggc
     5881 gctgtcgtac accttagagg acggctttcg cccagccaga acctccggat tcaccagcga
     5941 accggcatag atcaaggtgt ccgcatggtc gatgagccgt tttcctttga gcgtcagcag
     6001 ttccgggtct ccggcgcctg caccaacaaa atataccatg tcattctcct ctctgccgaa
     6061 tcagcagggt cgtaaaatag gaatacggtt ctgcctgggg actgcccaca tattccgatg
     6121 ccatgcccac atcccggagc attgtggtgt gttccagaag tccccgttct tccagcagcg
     6181 gcaacagcca ggacagcgcc cttccggctt tcataatcac cagattgtcg aattcatcga
     6241 acgcctgttc caggacttcc cgggatgcca cgccgggcaa gattcccagc gtctccccgt
     6301 tttcgcaaag gctctgcttt gccttggctg cgccggcaca aaaggacggc acaccggcgg
     6361 cgacccgggt ctcgtatccc ttttcctcca aaagctgccg cacataggat gccgtgctgt
     6421 acacggagac atcccccagc gttaccatcg ccaccagctt tccctcgtcc aatgccgcac
     6481 agaccggctg caaaatgccg gagcgcaggc gttcccgata atccgtatgt gctttcatgg
     6541 gaaacagcat ttccaggatc tgcgcacggg acaagtctgc cgcctgcgcc gctatggtgt
     6601 atgccgcaga tgcgctgccc tgtttggtga ccggagcgac caccacatct gcctgttcca
     6661 gcacctgctt ggcacgcagc gtcatatcca gcgggtctcc tgccccgacg ctcacggcgt
     6721 aaaatattcc ttttcccatc gcttactcct ttgtcataca tgaaaataac agcaccgaat
     6781 gattgctttc cagaatgtga taattcccca ccggtctgcc gcagctgact gccagctgta
     6841 tcacctcaaa ctgcggcagc ttttgcagca gcggaaacag ttccgcctgg gtttccaggg
     6901 tcactgcgct tgccacaaga cgcaccggct gtttcaattg gcacagatgc tctatcagtt
     6961 ccggtgctgc gccgtcactg ccgcccagaa agacacagtc cggcacaggc agactgccga
     7021 tctgctcttc cgctctgccg ggaatgatct gcaaattttc cgtctgaagg aatgctttgt
     7081 tcttttccag gatttccagc gccgccggct tatattccac ggcatacacc gtcccaaagg
     7141 gacacatccg ggcgcattcg atggatacac tccccgttcc ggcacccaga tcccagacca
     7201 ccgcatccgg tttcagccgc agcttggaca gcaccaccgc ccggacttcc tctttggtca
     7261 tcggcgaacg attccgcaga aacgcactgt ccgggagcag tcccaggcgt gccgtcggtt
     7321 tcgggtgcgg attgcgcacc gccgccacac agagtttcgg attcggacgc tgtgccgcct
     7381 gttccggcgt gccgtgccag agctgctggg tgggataggt cagatcggcg ccgacaaaga
     7441 tctccgtatc cgccagccca tatgccaaaa gcgactgggc aatttcccgt ggcccctgcg
     7501 tcccggaaca gaagaaaaat gtctcagcgt gttccgacac ggcataagca atcgtccccg
     7561 gactgcattc ccgtccgtgg atgctgcgaa tctctgcctg ctccatggtc agcccgaacg
     7621 ccgcacccag cagctgtaat gaaccaacgc ctggaatcac ctgcatttcc agttccggat
     7681 atcggttttg aatcgtgcgg cacagactgt acagcagcgg atcaccggac accacaatgc
     7741 cgatgcgttc ggtttccagc agttcgggga gttcctgcag cagccgggaa atgtttccca
     7801 tcggtctcgc ctttttgact tccagcagtg acagaaaccg ctccgatgca attacacagt
     7861 ccgccgactc cagcgctttt tttgccgccg gcagcagata atccgctgtg ccgctgccgc
     7921 cgcctgccaa gatcagttca tgctgcacgc caaccactcc tttcgtattt gcgccgcccg
     7981 gctgctctct gccaggatac ggtcggaatc atccagaagg atcagtccca cctgcaccct
     8041 gccatgtgtg cgccgcatac agttttcaca gacgctttcc gccacggact gccacacctg
     8101 ttcgccgaat gggaactgcc gcagcagttc tgtcatttcc cgtgtggtgt tgcactgata
     8161 cagctgccgc acctgcatct ggtctgctcc ggcaagcgcc gcatgggtgc aaagcacctc
     8221 acgctgtccg ccggcggtgt ggctgtgcag gtacatgata tctgccgccg gttttaccag
     8281 cttgcccggt ctgcctgcca gcagcacgcc ggtaaagccc attttctcgg cacagtccag
     8341 cagaaagccc agataattgc tgcaaaggac gatcggtccg gcatccggat actgccgcct
     8401 cagccaagat tctccggcgt atcccgtcac aaaggcacac acctttccat actctgcccg
     8461 gcacatggaa agttccagcg acagcgactc ccggacagcc tcttcgctca tagggcgcac
     8521 aatgccggtt gtccccagaa tggagatacc gccttcgatg cccagccgtc cgttaaacgt
     8581 tttttgtgca atgcgttctc cgtccggtac ggaaaccgtc accaccgcac cctttgcgcc
     8641 gatcacagag cgcacagcct tttcgatcat ctctctcggc acaggattga tcgccggctc
     8701 acccggcgga agtttcagcc cttttcgggt cacaacgccc acgccgtctc cggcacggaa
     8761 ctgcacagcg ccgtcctccg gcagaatctg cacagcggca tttaccagac agccgttggt
     8821 ctcatccggg tcatcgccgc cgtcttttct cacgccgcag gtgtacgccg caccgggctg
     8881 tatcgccaga cgcagttttt ttccggcagg cactaccagt tccacccatt ccggacagct
     8941 gcccgtggcc tgccacagca cagatgccag gcttgcgccg gcagcacagg cgcctgtggt
     9001 gtatccttcc cgcagccgtt tcattccggt tttccccatt gttccaggat ggcgtagatt
     9061 tgctccatgt ccaggttttt tcggagcaga tctgccagct tgtcgtactc ctgctctttc
     9121 cgggcagcaa agtccagctg cggaacagct gtcccggaga tgcccttttt ctccaacagc
     9181 agctgcacca gctttgccga aaattccggc gcatcaaaaa tgccgtggag gtatgtgccg
     9241 cagactgtcc cgtccgcact gacacagccg tcagctctgc cgtcctgcaa atgcacaaaa
     9301 ggcacaccgt ttccggtgca gactgtttcg cccatgtgga tctcatagcc ggtgaccgga
     9361 caaccggaaa gggattccag cattccggaa acgccggaaa tcacgccggc tgtctggctg
     9421 cgctgtttct gagaccgaaa gaccgtttcg gtctccagca agcccatgcc acgcagtgtg
     9481 ccgccatctt cggcgtgttc cgggtcagaa agcgtctgcc ccagcatctg aaatccaccg
     9541 cagatgccca tgagaaagat gcctttttca tgacacttga gaattgccgc ctccagaccg
     9601 ctctgccgca gccaccggag atctgccatg gtgcttttgg ttcccggaag caggatcaca
     9661 tccggcattc ccagttcgct gcgctttggc acatagcgaa ccgaaacgcc gtccacctgt
     9721 tccagcgcct caaaatcggt gaagttcgag atacggggca gacgaatcac agccacatcc
     9781 aaaagtgcgc tttcgtctgc ttttttctgc atcagccgtt ctgaaaggct gtcctcatca
     9841 tccagttcca gcggcagata cggtacaacg ccgagaaccg ggatgccagt cagatcttcc
     9901 agcatcttca gccccggttt cagaatctcc acatcaccac gaaacttgtt gataacaacg
     9961 ccacggatgc ggcggcgctc cgcctcgtcc aggagcataa tcgttccgta gagcgccgca
    10021 aacacgccgc cacggtcaat gtctcccacc agaattgccg gagaatcgga aaactccgcc
    10081 atgcccagat tcacgatatc caccgatttc aaattgatct ccgccggact tcctgcgccc
    10141 tccagcacaa taatatcatt ttcttctgca agtgagcggt aggcacgcag gatatgcgga
    10201 acgagttcct gctttttcgt gtagtactcc tgcgcacgca tcgttccgta aatttctccg
    10261 ttgacaatga cttgtgagcc gctgtcgctg gtgggtttca gcagaatggg attcatccgg
    10321 gcatccggct gaacgcctgc cgcctctgcc tgcatgacct gcgcccgtcc catctccttg
    10381 ccgtcctgtg tgatgtacga attgagtgcc atgttctggg acttaaacgg cacggttttc
    10441 cagccgtcct gccgaaagat gcggcacagc ccggcagtga taaagctttt tccggcagag
    10501 gacatggtcc ccagcaccat cagcgatttt gccaccacaa taaaacctcc tccataacct
    10561 gccgcagaca acgcagcaga tacgcatttt cttccggcag gcgaactgcc gcacggtaat
    10621 cgtcgacgcc cagtcctaca tagttatcac agtgccgcag caaaatgccg tacggtatca
    10681 gccgctcgtc cagatcgggg catccctctg cccggaaaaa gacaaagttg gcgctgggtt
    10741 tccatacccg aaatcccagc tttcccagtt catcgtacag ccgccagcgc tcgtcctgca
    10801 aaaacgaaag cgatttttcc cggtattctt ctgcgtgcag tgctgcgatt ccagcctctt
    10861 ccgccaacgt atttaccggc cagggctgcc cggtctgccg gatcttttcc acaagctctg
    10921 tatccgcaca gagtccgtag cccagacgca gtcccggaat ggcatacagc tttgtcagcg
    10981 atttcagcac caaaacgtgc ggatactgtt ccagacaggg cagcatggaa cggattcccg
    11041 gctctgtttc tgccatatcg caaaagcatt cgtccagcag cacaaagatg tgtttttctg
    11101 cggcaaggca cagaatgcgt tccagcagtt ccggtgcaat tcccgttccc gtgggattgt
    11161 tgggattgca gagcgccaga aaatcataag agccgcattc cagcgctttc agaatcgtct
    11221 cgtcaagctg atacggaaac ggcattttcc agtaggtaac acggcatccg atttcagcaa
    11281 gcgccgccgc atattccgaa aatgccggca cgggaatcag tgcgtggctc ggcgacacag
    11341 cacgaaccgt gcggaagatc acatctgcgc cgccgtttcc gcagacgatc tgttccgggg
    11401 ttttgccgtg aaattctgcc agagcctgcc gcagtctgcg ctgtgccggg tctggataat
    11461 gcaccaggaa atccaccgca ttcacgatcg cactgcgcac cacatccggc acgccaagcg
    11521 ggttaatatt ggcagaaaaa tccagcaacg gtttctcctg cttttgaaag ctgtaaatat
    11581 caccgccatg ccggggcata ggcacacctc ctcatcgaaa cagcagcact gcggcaaacc
    11641 gcagcagacc aaatcccaaa agcgacaaac cagacgccgc aaacatcatc ccattggtgc
    11701 ggcgaatgtc ctctggttct gccaaacgaa ccgcatcgcc gatggtgggc ttttccacca
    11761 cgtcgccgaa atagatatgc gttccgccca gctggattcc cagcgcaccg gctgcggcgg
    11821 attctgtctg cgcagagttg ggagacagat ggtttctccg gtcacggcga aatatacgcc
    11881 aggcgttttt cccgtccagg cgcagcaaaa aggcagaaac cgtcagcagc agtccgcaga
    11941 tacgggacgg cagccagttc gccgcatcat ccagctttgc agatgccctg cccaagtgaa
    12001 gatattttgc attgcggtaa cccaccatag aatccagcgt gttgattgcc ttatagcaaa
    12061 atcccagcgg cacaccgccg atcatcagat acagcagcgg cgcagtcacg ccgtcagatg
    12121 tgttttccgc tacagtctcc acacaggcct ttgcgacctc ttccgccgtc agctgagtgg
    12181 tatcacgtcc caccaggtag gaaagctgca ctcttgcacc gggcagatcg ccatccgcaa
    12241 gaaggcggta caccttgtcg ctttcccgtt ccagacagcg tgccgcaaga atctgatacg
    12301 cccagaatgt gtgcagcaca aaccagagcc agaacgaaat gcggtacgcc aaaagcagaa
    12361 tgcccagaga gagcaacaga cttcccagcg gaatggctgc cgccagaatc accccggcac
    12421 ggcgttcccc ttccggagtc tggggaaact tccggcgcag aaaccgttcc aggcgagaaa
    12481 tgctttttcc gatcagcacg accggatgcg gcagccaccg gggatctccg aaacagagat
    12541 ccagcaaaaa tcccagcgct gtactgcaca agatccacac agactttttc ctcctacttc
    12601 aatcgcattt ccaagccgca gcagacacgc tccacagtct ccgcagattc tgccagcaga
    12661 cagttcaaac gtcccactgc ctcacgaaac cggcgctgtt ccggttcgac cggcacaagt
    12721 ccgcagccca cttcctggct gatgaccaca gcgtctttcc atttttccag ccagggctga
    12781 atttcctctc ccggatcttt ttcctcggtc agccagcgtg ccgccaaaga ttccacgtgc
    12841 agaatcagcc gctgtccctg ccagtctatc aagggctgct gcgacgcatc aaagcagtcc
    12901 tgcgccgtca gctgatactt ttgcatggca taggctgtcc tgccgttggc atagccgccc
    12961 aggatcagtc tcatgccagg acacctccca gcagtgccgc acccagcagg cagaccgtct
    13021 cacacagcac caggaaaaag cctgccagat cgccggtaat gccgccgaac tgtttcttcg
    13081 ccatatgcga acagccgaag aaagcgaaca gcccgatcca cgccagaacg accgccagcc
    13141 aatcccagaa cagaacagtc agcagtccca caacgccgta cacaatgacg agaataacac
    13201 gcacgtgttt tccggcgctt tcgccaaagg ttttcgccag tgagctggac tgcgcaatgg
    13261 gcagtgccgt aacggacaag ccgctgagac aacgtgacaa gacaaatacc cctgtcagca
    13321 gccagacaaa actgcggttc tgggtctggt agatctgcac atatgcgcca aagctgacca
    13381 gcagtgccag aactacgctg aacacggcaa acggtccgca gttggggtct ttcagaatgc
    13441 gcagtttttc ttcctggggc cgtctggaat acagcgcatc cgccgtatca cagaatccgt
    13501 ccagatgaat gccgccggta atcagcacgc cgctcagcac tgccagaacg ccgaacagca
    13561 ctgcgcccac gtgcagcagt tcacagaccg tccataccac aaacaaaccc acaccgcaca
    13621 gcaatcccac cagcggaaaa aacaccatgg cattgcgcag gttcttttcc gtccagttca
    13681 gacacttcac cggaagtgcg ctatacatgg aaagtgcgat cagcagggat tccatccagg
    13741 attttatcat attgttcttc tcctttccaa atcagcggac aaccggcaca gcattcgatc
    13801 acaacatccg cctgtgctgc gatcctgcag ttgatgcgtc ccagatattg cagatactgc
    13861 cgcatggacg catcttccgg cgtaccatcg gaaaatactt cgttcgtgac gatcaccagg
    13921 tgctgcacct tttgatacag tgcagcaatg ccgtccagaa cagtctgcgc cgggtcatct
    13981 gcaccctcgc caaactgttc gttggcaagc agattgctga gacattccag cagtgccgta
    14041 ccgccgggag aaagcgtttc cgcctgttct gccaggtggg acggaatctc cagcgtccgg
    14101 aatccttttc cggcacgctg ctgccgatgt ttggcaactc ttgccctgca ctctgcatcc
    14161 cagatctgca tggtggcgat atagcgaaac ggctcttgtt tcagtgccat gctgagacgc
    14221 tcggcaaact gagactttcc gctgcctgcg ccgccggtaa ccaaaatcag catgacaacc
    14281 gctcctttcg gtacgcctta caggcatctg caaaatgctg cggcacttcc ggatttgacg
    14341 gaaaatacag gtgcggaaat ccggcgatga tctgcgcctt tgtctgcact gcctgccagg
    14401 ttcttccgtt cgggcgctgt gccagaaatg cactgccgtt ttccgtactg tccgcatagt
    14461 ggaattcatg cgcccggatt ttcgttcctg ccttgcccag gatcgtgtcc tgctgtgccg
    14521 tcagcgttac ataaccaaaa cggcaaaggc gttcgcccag acgggatgtt cccggcagaa
    14581 gtccggccag cggataggac ttgccgtctt ttccggtcat agaccgctgc aggtacagga
    14641 atccgccgca ctctcccaga atcgggatac cagtgccggc gagccgccgc aggctctcca
    14701 aaaaggcagt attgccgctg agcctgggca ggtacagttc cggatagccg ccgcagaaat
    14761 acaaaccgtc caggttttcc ggcagctttg cgcctgtcag cggtgagaaa tataccagtt
    14821 ctgcgccgca ttgttccagc agttccagat tttccgcata ggtaaagcag aacgccttat
    14881 cctgtgccac gccaagccga aacagctttg gtttcgtcgt atacggcggc acatccggca
    14941 gcggcggcgc tgttttcgcc agttccatga tacgggaaag ttccagggtc tctgcggcgg
    15001 tttctcccag caggcgtatc ttttcgtcca gctgttccac ttccccggcc gtcatcagtc
    15061 ccagatgacg gctttccagc tgcacttccg gcagatgagg cagatagccc agcacaggga
    15121 gtccggtctc ccgttccagc agttcccggt aataattgta cattccgctg cggatatcgt
    15181 tgagcagaat gccgcagatg gaattcggtg tgcggaatgt ctgaaacccg tgacaaagcg
    15241 ccgctgcaga acagcccatt cccttgggat tcaccaccag cagcaccggg attttcagcc
    15301 actgggaaac ggtataggcg ctcgcctggt cggtctgtcc gatgccgtca taaaaaccca
    15361 ttgcgccctc aaagacggca cagtccgcat cggcactcac ccgggagacc agctccgcca
    15421 tcggctgttc tgacagaaaa aaaggatcgg cgtgatacgc cggtctgccg gtgatatgac
    15481 tgtgaaacat gggatcaata tagtcgggac cacacttcca gccctgcacc tgcagcccac
    15541 gggaccagag ggcagacagc agtgccgcag tcattgtggt ttttccagca ccgctccctg
    15601 cggcgctgag cataatgcgt ggaacaggct gtggcatggc aaggtctcct ttccggatca
    15661 attttgaata tatttgaatg tattttatat tataccacat ccggacgttt tgttcaagcc
    15721 atccggacac acattttatg tggatttatt tttgcgtcaa aacgatatca acagttccgc
    15781 cgaattcttt tccttgacaa tggaatccaa aggacttata atagggacat catcaaaatc
    15841 gtacaaatta tgcacgattt tgaaaacgat accacagttt ctgcaagcag gtttgatttc
    15901 tatatagttt tgtacgattg ttatagtgat tcctatgaat gttatagaaa tagcgatttc
    15961 tttatcttat aatatcattc ggcatcagga taaaataaac gtcctgaaat ataaaaatag
    16021 ataaggagac tatcactatg gcaacacaaa acactcgtgc ggaattccgc aaaaagctgc
    16081 tgcacctcat gatcccgatt tccctgcagc agctgatgat ggcgatggtc aaagcgtcag
    16141 actcatttat gctgggcttt gtcaaccagg atgccctctc ggcagtatcc ctggcaggac
    16201 aggtgcaatt cgtatattcc ctgtttttgt ttgccattac tgccggcgtt tcgatctttg
    16261 ccgctcagta ctggggcatt cagaacaaaa aggcagtgga aaagacgctg ggcatcggtc
    16321 tggcattttc cgtgctgctc tcccttccct ttaccatcgg cgcaacattt ttcccggaac
    16381 tgctgatgcg tgcattcgcc tctgatccgg ttctcatcca ggacggcgct gtttatcttc
    16441 gggtagtcgg cgtgtcctat ctgctgctca gcatttcgca gatctacctg tgcattctga
    16501 aaaactgcgg aaaagccgta ctgagcgctg tcatcagttc cgtttccgtc atcatcaaca
    16561 tcattctgaa tgccgctttc atcttcggca tcggtttctt tccggaaatg ggcatcgccg
    16621 gcgctgccgt tgcaacggtt atcgcaaagg tgatcgaact ggcatgggcg cttgcagtga
    16681 tgctgaaaaa ggacagcatc aagatccgcc ttggctggac catccacccg gacaaggtac
    16741 tgctccgtga cttctggaag tacaccgctc cgatcctggg cgatgagctg atgtggggac
    16801 tgggctttac catgtactcc gtcatcatgg gacacatggg cagcgacgcc gcagccgcaa
    16861 actccattgc aaacatcgtc aagaacctgg taatctgctt ctgcaccggc atcgctaccg
    16921 caagcggcat tatggtgggc agtctcctgg gacagggcga actggaaaag gcaaaggaat
    16981 acggcagaaa gctttccaag acttccattt ttgcaggcgc agttgccggc gtgattattc
    17041 tgggcgttat cccctttacc cctatgttct ccacgctgac cgacactgca aaagaatacc
    17101 tccgtgtcat gctggtggta tgctcctatt atgtagtcgg caaatccatt aacatgacgg
    17161 tgatttccgg tatcttcgca gccggcggtg actcgaaatt cggctttatc tgcgacggca
    17221 tcaccatgtg gtgcgttacc gttcccatcg gtctgatcgc tgcatttgtg ctgaaactcc
    17281 cggtactgac cgtatacatt cttatcaacg tcgatgagat catcaagctc ccggcagtcc
    17341 tcaagaacta ccggaaatac cgctgggtca agaatctgac ccgacaggaa actgcacctg
    17401 ttgaaccaga aaccatggca cagacagccg cataacacaa agaaaaggag tcctctatgt
    17461 ataccctgga actgacagac aacaagtctg agatcatcca atacaacgta cccgatctgc
    17521 ccatcaaggc atcccgttcc tctcaggagg actatccctc gctttccatc gtcaaccact
    17581 ggcacaccga gtttgagttt atctatgtga aaaatgcacc catgtggtac agcgtcaacg
    17641 gcgaacagtg caagctgatg ccgggacaga tgatttttgt caactccggg cagatgcact
    17701 acggtttctg ggaaaaaccc agcgattccg tctatgactg caccctgttt ccaccgtccc
    17761 ttgccaccaa tcccacgacc aaagatttgc tggaggacat catccaccat gcgccgccgt
    17821 atctcattct ccacccggag atcgccgcag aaaaaacagt tatcacgctg gtgagtgaga
    17881 tgttccagtg tgccagcaat cagcagaacg gctatccgct ccagcttttc ggctgcatct
    17941 accgaatctg ccacgccctg tggaacttaa tgcagaataa cgctgagccg tgggagccga
    18001 acgacagcaa gcgcctggag gcaatgcaca agatggtggg attcatccag cagaagtaca
    18061 gcagcaagat tgctttgcag gacattgccg ctgccggata cgtctgccgc agcagctgct
    18121 gttccatctt caaggaatac ctcaaccaga cacccaacgc ttatctgacg gaataccgga
    18181 tctccaagag cgtggaactt ctcgcaaacc cggatctgag cgtgacggag attgccatgc
    18241 agtgcggatt cagcggttcc agctacttta cagagacctt ccgcaaagcc atcggctgca
    18301 cgccgtcgga ttaccggatg cgcaagcagc agcggcggta aacttaaggc aacaccgcac
    18361 agagtttgtg cgtaagatgc gcagtcggat tcttcggcag attgcacaga aatttcgcag
    18421 gcatactgtc ggtcgcaatc aatcctagtg cgacactcct ttgttctcgg gcagaatcct
    18481 gcctcgaacg cagtcgtatg tcaagaaatt tctgtgcggt atgacggaaa atcagcaagc
    18541 agattgcgtg aaaagccgtt aggcgggctt tgtgcggtgt tgccttatat tctttcaaac
    18601 aaaaaagcaa tattgcgccg gagttttccg gttgcaatat tgcttttctt ttttctcaaa
    18661 tttcaggcga ttattctacg ttcttcagcg catcgctcat gggaatcttc ttgaccttgt
    18721 ggttcagcag gaaggttacc acagcatagc agatcacgcc ggcagcaatg attttcgcat
    18781 atgtcaccgc cggaacatag taaacgatcc agccggagta ttcaaagaat acgatcttca
    18841 tgacctctgc cattacccag ttgcaaagcg gtacagtcag cagaatgcag atcagtacca
    18901 caatggtggt ggagtgcagg tagatacgat tgatttcccg gttgctgtag cccagaatct
    18961 ttaccatgga aatggactgt gcattcttct caatgatgat cttcgacagc agatacagta
    19021 tcagaatgaa catcagcgag ccgaatacca ggaacagact catcatgctg cccatagagc
    19081 gaatcagctg acgagatgtc tttgtcagat catccacagt gatttccgtg gcaatcgctg
    19141 catcatcaat gtctgtgatc tcctcatccg agaagtacgc attgaagtag gtatcatctt
    19201 tgtcaaacgt ctcattataa aggctgcgat ccatgaacac ggcaatggtg gatggataat
    19261 catagatacc gccgaccttg aacttgtact tgtcgctgcc gtacgggtct ttcagggtaa
    19321 tggtatcgcc cttttctacg ccgtactttt cagaatatgc gctggaaatt tccacctcat
    19381 cgtccgaaac atccagtttc acatatttgc tgtccggctc tacgccatac agggaaacac
    19441 cctcactgct gcgttcctcg cctagcgtgt ccagactgct gaggcagaac ttttctgcgc
    19501 catcttccgc tgtttccacc ggtgctttca gcacatactg gtaggtggac agcgcattgt
    19561 ctgtgaccat atcctggaaa tgtgtcagca tcggatgcag aatcataccg aacatgagaa
    19621 taaagtttgc gaaaaagata ccgaaaaaga tcatgatatg attcggcagg ttctggaaaa
    19681 tcacacgcag ctgaaaacgg cgcatgatcg ggatacgggt gttcagacgg aacgcctttt
    19741 tcttcttccg ctttgtcaga tcacgccgga tgaatttcag cggcgaaatg cgcattttta
    19801 aggtcagcat gacaaagttg atgaggaaca gcagtaccag cggaatcacc gtggtcagca
    19861 ggaatgcctc cggattcagc agcacctcat acgtcggcag gctgtagctg cccagatact
    19921 ggtcagcaaa tacctgttcc aacagggtgt atcccagaat attgccgacg atcgccgcca
    19981 caagcagaac cagcattggc ataataatgt agtgccgcac catttctcca cgggagtaac
    20041 cggaggcacg cagcgtaccg atgacattcg cctctttggt gatgttgttg gaaatcgtga
    20101 tggaaaagac aaacgccaga atcacaacaa catagaggaa tgccataatc atggacttat
    20161 cgccgcccat gtcgtcacca gtaaagatga tcgcctggtt cagatattcc ggcaggaagt
    20221 tagtcagcac accgttttgt cccagaatcg gcatcagatc ttctgccata tcctttgcct
    20281 cagcatcatc ttccggcgaa tcatcataga accaactgta gctgtagtgg atgtgattgt
    20341 cccgcagcgc atcaaagccg tcatcggtca taacgccgac accgaattta tcgttgtcaa
    20401 acatcatatc cgatgtgttc tgaaacagcg cagaataatc cgagagggct acgataccgg
    20461 tgatatgcag cgttgtatct gccacacgga aatcatcgcc gatctccagc tcatggtttt
    20521 tcgcatacag acgatcgatt gctacgtcct gctctgcctc gggcatatcg ccgtccagca
    20581 ggcagacctt atcgatatcc tttcgctctg cgaatacccg gaatgtactt tcgaaatcat
    20641 ccgtcttttc ttctttataa aagttcgggt acaccttgac gctgccgtct ttctcgatgt
    20701 tggcaagtgc ctcatctgtg gcagactccg caaattcaaa gttgccgtcc tcgatgttgt
    20761 acttatcaaa gctttcatga taggaagcga tcagactgtt gtctgccacc agaaatccgg
    20821 agacaaggga gatggacatg acaaaaaaca aaaagataac cagatatttt cccagctcgc
    20881 ctttcaattc ccggggcaga cgcttgttca gtggattctt tgttctcttc gccatggctt
    20941 accactccaa ttctgctgcc ggaatctttg tctcgttgac aaaattcttt cggatctttc
    21001 cgtcacgcag ctttaccaca cggtctgcca tattctgcaa agcgtcatta tgggttacca
    21061 tcacaacggt atttccatac ttgcggctga catcctcgat cagtttcaga atatccttgg
    21121 acgtgctata gtccagcgca ccggtcggct catcgcaaag caggatgtcc ggattcttga
    21181 cgatggctct gccgatggcc gttctctgct gctgaccgcc ggaaagctgg ttcggcagct
    21241 tatgccggtg ttcgtacagc cccagggttt tcagaagatc gtccacatcc agcggtgaat
    21301 cgctgaggta tgcgccgacc tcgatgtttt ccttaatatt caggtttggg atcagattgt
    21361 acatctggaa aatatagccc agatgtttcc gccgatacag cgtcagactc ttctcattca
    21421 tgtccccgat ggtctcgccg tgaatggtaa tgctgccgct gtcgggcgtt tcaatgccgc
    21481 cgatgatgtt cag
//
LOCUS       DAWENO010000018        20339 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1103_length_20339_cov_11.569661, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000018 DAWENO010000000
VERSION     DAWENO010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20339
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_20339_cov_11.569661"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1758..1767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5265..5274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4563 a         5334 c         5884 g         4538 t
ORIGIN      
        1 tcacacgccg ccgccagctt tgcatacagc gcatggaaaa agtccgtgtc ggcggaggcg
       61 ctgacgtgat acgccacgcc ctttccgaac tggtttttcg tcagcaccgg ctttccggcg
      121 tagaagtccg tttcatacac ggcgagcgtt tcgcaggtgg tgggatggca gatctcgcac
      181 agctctttca gcaaatacga cttgccgttc caggtggtgc ggttggattc gcagtcatac
      241 agactgtcca cttcctccac ccacagcccc agcacatcgg aaagcttgtc ctcggtcgcc
      301 tcgcccagaa agctcaggtc ggtgtcgttg accacgccgg tgaaacaggt ggttaccagc
      361 gtgccgccgt tccggacgaa ccggcgcagc ttttcctgga tgccgtcccg gaacaggtac
      421 agcatgggtg cgatcaccag cttgtagccg ctgaggtcaa aggtgctgtc gatgatatcc
      481 acattgatgc cgtttttcca gaaatagctg tagttccggc ggatattccc gaaatagtcc
      541 agcccgatgt ttctcggccc ctggcatttg tccagcgccc atttgttttc cgtgtcgaac
      601 aggatcgccg tttccgccgg cgtgccgctg ccgtataccc ggtcggacag ctgttccagc
      661 acagcgccga tgtcggtgac ttcccggaat gtccgggtgt tttccgtgcc ggtgttggag
      721 atgaccgccg agtggaactt ctcacagctg ccccggctct ggcgcagctg gaagtactgc
      781 ccggaatctg ccccgtgcgc caccgcctcg atgacggaaa gctggtgcat ccccggcttt
      841 ttcagcttgc tgacgccctg ccagttggtg gcgttcgggg tgcattccat gagcaggaac
      901 ggcttttttt gcagcgaccg catcatatcg tgtgtcatgg cgcagtcctt cgccacttcc
      961 tcgttgccca tataggggtt gtgccattcc ggatagttgt cccaggagat cacgtccagc
     1021 gcatcccgga aggcgaaata gttcagttca taggtgtaca tggtcatcat gttggtggtg
     1081 accggaatgt ccgggttcac cgcctttacc gcagcgatct ccgttttcat aaagtccacg
     1141 gtctggtggg tgacgaaccg ccgccagtcc agatccagcc cgtgaacgga agtctcaccc
     1201 accgggctgg ggctgtgtac ctgtgaccag tccgtatagg tgtgcgacca gaagtttgcc
     1261 caccaggcgt ggttcagctt gtccagcgtg ccgtattttt tctgtaccca ttcccggaac
     1321 gccgcctggc acagctcgca gtggcattcg ccgccgaact cgttagaaat gtgccacagg
     1381 atcaccgccg ggtggttggc gtagtgttct gccagcaggg tgtcgatttg ggtgaccttt
     1441 tcccggtaca gcggcgaggt gtagcagtgg ttctggcggt gaccgtagaa gttccgcagc
     1501 ccgtctggac ggacacgcag cacctccgga tatttctgcg ccagccactg gggtcttgcg
     1561 ccggagggcg tggcgaggac ggtgtagatg ccgttggcat agagccggtc gatgatctca
     1621 tccagccagc cgaagtcgta aacgccctct tccggctcca gctttgccca ggagaaaatg
     1681 cccaggctca cacagttgat gtgggctttc cggaacagcc ggatgtcctc ttccagaacc
     1741 tccggctgat ccagccannn nnnnnnnaaa tgtcggggtc gccggtgcaa atctcggtgc
     1801 catggttttg aactcctttt ttcgtctgaa tgtacaaaaa gtgcattcga atcgtttcgt
     1861 tttttgatag ttttattgta acacatttca aaacaaatag caagggcaaa tgcaaaattc
     1921 tttttgggaa tcccggcgga gcaggcgatt tcagcagttc gacgaagttt cggcgcaaat
     1981 tttggcgatt gttcacaaat acggcgaatc tgcgccgcag aacgtcccat ttcgccggaa
     2041 agcgggagat tccggggaaa agcggcaaaa tgtgaaaacg attcccttgc ggttttcagc
     2101 cgcttttcag cgtgatttca cgaaatgaac acattgaccc tgtatactaa aaccatgctc
     2161 aaagagagca tgtaacatcc tctaaaccgt ttcgcataca ccccgatgcg gaacggaaaa
     2221 tccccaataa tccctatatc atagcaaaaa acgtccctca ccccggggcg ttttttgttt
     2281 tatctaaaaa agtctctcta acagttcccc ttgaaaaggc aaccgtaggg cgtgccttgt
     2341 actgaactgg taaatatagg ctcccctaac aggggagctg gcaagccgta ggcttgactg
     2401 aggggtgaat cccccttttc aagggggact ttgtttccct tgactgaggg actcattttc
     2461 ttgtcaaccg tgcctatgga cttttcgtat cacatatgtt ataatagata cattctacag
     2521 aaatacaggg atgtcctgtg tgtgaaagga cttatttcat gaaacgtgtc gtttttctcg
     2581 gtgcagcggc ggcaacgctg gtgcagttta ccaaatatga cttgaccgca ctccagacgc
     2641 tgggctgcga gatccacatg gtctgcaata tgcagaacgg caatctctca gaggctgcgc
     2701 tccacgaata caaccagact tttccccagc tgcactggca cgacctgccg tttgcggaca
     2761 gcgccgccgc actgcgccgc aaccaccagg cataccaaaa gcttttgccg ctgctgaaag
     2821 aactgaaacc cgatctgctc cactgtcacg gcacgatcgc cggacagtac ggccgccgtg
     2881 cggcgcagga gctgggcatt cccgtgtttt acactgccca tgatttccgg gtgtttcacg
     2941 gctgcctgct ggcagaacgg gtgtatttct cggcggtgga acgcaaatac cggaaagcga
     3001 cagaggtgtt ctttgccgta tgtcccgaag atgccgctta tgcgaaaaag catctgaacc
     3061 aggcggaggt agtctctctg ccggatgtgg ggctggactg cgacaaatac gccatgccgg
     3121 agcgcagccg ggaagtggtg cgaaacgaac tggacattcc ccaggatgcg gtggtgctgc
     3181 tctcggtggg aacgctccgg atgcagaaac gtctccgggt gatcctccag gcaatggcac
     3241 gcctgcggaa ccgggaacag ctgcactatg tgatctgcgg cgaaggaccg gatatgccgt
     3301 ttttgcagaa actgactcgt cagctgcatc tggaagagcg tgtccacctg ctgggctacc
     3361 ggacggatat cccggatatg ctgggcgctg cggacatttt ctgtatgccg tcccggcgtg
     3421 agggctgcgg catggcggcg ctggaggcaa tggctgcagg gctgccgctg attaccgtgc
     3481 gcagccacgg cacaaaaaca tatgcggaaa agggcgaaag cggcttttgc ctcaagggtg
     3541 acctggtcac cagctgcgcc gacgccatcg aacagctggc ggataacaag ctgctccgcc
     3601 ggcagatggg cgcacgcaac cgtgccgccg ccgccgcctt ttccgatgca gaccgcatga
     3661 tgctgctgcg tgggtactat caaaaggcgc tgggggatta aaaccccgtt ttcgaaagcc
     3721 ggacacattc tccctaccgc tgcatacaat acagtgagga ggagagaccc atgcagtcaa
     3781 ccaaatctgt ctgtccgctg tgcagtctgg cgtatggaga gaccgcagtg atcgccagcg
     3841 tctatttgca cggggcgatc cggcggcggc tccaggatct cggctggatt cccggcacag
     3901 cgatccgatg cctgcaaact gccggcggcg gagaccccac tgcttatgct gtgcgtggcg
     3961 gcgtggtcgc cctgcgccac cgggatgcaa aaatgatcct ggtacacaaa aatgattctg
     4021 atacaataga atgacagaaa aaaagccccg gaaaggggct ttttttggtt gcgatgaaag
     4081 tggtatgtta tgcctggtgg actgcgtctg ccagcgccag cagtgcggat tcgtctcccg
     4141 gctttaccgc agaacggatg gtgacggtct gttccaggac ggtgaggtcc ttcatggtgt
     4201 ccaggagcgt gtgcattttc tttgcggcac agggcgccca cgagccgttt tccaccagag
     4261 cgatggtgcg tttctggaac gctttgtatt gcaggtggag caggaattct tccatgggca
     4321 ggaacacgcc gccgtcatag cttgccgccg ccagtactgt ccggtcgaac cggaacgcct
     4381 gtgccacagc ctctgccatg tcgcaccggg acagatccag cagcaccacg gtttcgccca
     4441 gggctttcag ccgctctgcc agcagggcag ccgcctgtgc cgtgttgccg tgaatagagg
     4501 catacgccac cagcacgccc ttttcctccg gggcatagct gctccaggtc tggtactttt
     4561 ccagataggg cgcaaggtcg ccggtcagca ctggaccgtg caggggacag atggcgcaga
     4621 tgtccagctt tgccgccttt ttcagcagcg tctgcaccgg tgcgccgtat ttgcccacga
     4681 tgttaaggta ataccgccgt gcctcgtctg tccagggctg gtcggtgtct agcgccccga
     4741 acttgccgaa agcgtctgcg gagaacagca ccttttcgcc ggactcatag cacaccatga
     4801 cttccggcca gtgtaccatg ggtgccatga cgaagtgcag ggtgtggctg cccagttcca
     4861 gcgtctcgcc ctctttgacg gtacgggtgg gattgcccgg catatcgccc aggaactggg
     4921 gcagcatccc cagcgccttt gcagagccaa ccagggtcat gtccggatag cgttctgtga
     4981 tcgcccggat gctgccggcg tgatccggct ccatgtggga gatcaccaga tagtccggcg
     5041 ctgtgccgtc cagcacctca gtgacctgtt ccagccactg gtctgtggca cggggatcta
     5101 cggtgtccag cagggctgtc ttttcgtccc ggatgacata ggcgttatag gagacgccgt
     5161 ccgggacggg atactggctc tcgaacagat ccagggtctt gtcgtctgcg cccacataga
     5221 agatctgctc cgaatgggga atcgttttca gcatggtaat acctnnnnnn nnnncttaat
     5281 aggaataatt ctcatttatt ttagtataac agggaaatgc aggaattgca tgaagttttt
     5341 ttgtgcattt taagaattct tcgggcgatg tttggcagtg ggattaaccc ctcagtcaag
     5401 cctacggctt gccagctcag cacaaggcac accctacggg cgccctgtta ggggagccta
     5461 tattataaaa tatctacctc aacacaaggc acaccctgcg ggcgcctttt caagggggac
     5521 ataatgcgag acaataagcc tcccttgaaa agggaggtgg atgctccgta ggagcagacg
     5581 gagggattat tttctatcag ctttagctga cagggggatt catgtttcaa tcaaatctaa
     5641 agcctcctct tgtagaggag gtggcacgca gtgccggtgg agtctttcgg tatctttcag
     5701 aaaaccggat ttccgcagcg ttcaaaaata aggcttgcat ttttctgcgg aatacggtat
     5761 aataagatga atcaatatgt ttgcatgaag cagaacttgt ggaaagccgg cgctccaggg
     5821 cgattttccg gggaaatggg ttctgacaaa cgggaggatt attaaatatg ccaaaatatg
     5881 tattcgtcac cggcggcgtt gtgtccggtc tgggtaaggg aatcactgcg gcttcgctgg
     5941 gtcgcctgct gaaagccaga ggctataagg tcaccattca gaagtttgat ccgtatatca
     6001 atgttgaccc cggcacgatg agtccgtatc agcacggcga agtgttcgtt accgatgacg
     6061 gcgcagagac ggacctggat ctgggtcact atgagcgctt tattgacgaa agcctttcca
     6121 tcaacaacaa tgtcaccacc ggtaaggttt actggaccgt tctgaacaag gagcgccggg
     6181 gcgactatct gggcggtaca gtacaggtaa ttccgcacat caccaacgag atcaaggacc
     6241 gtatttaccg tgtcggcaag gagtccaatg cggatgtggt catcacagag atcggcggta
     6301 cagtaggcga tatcgaaagc acaccgttcc tggaggcgat ccgtcagttc gtgaccgagg
     6361 tgggcagagg aaacgccatg tacatccatg ttaccctggt gccgtacatt gccggctcca
     6421 acgagctgaa atccaagccg acccagcact ccgtcaagga actgctgtcc atcggcatcc
     6481 agcctcacgt ggtcgtttgc cgtaccgagc tggaaattcc ggaggatatg aagcggaaga
     6541 tcagtatgtt ctgcaacgtc cgggaagaag atattattca gaatatgact gcgccgtctc
     6601 tgtatgaagt tcccatgatg ctggagaacg agggactgac cgacagcgtc tgccaccacc
     6661 tgggtctgga aaaccgtgag ccggatctga ccgagtggac tgcaatgacc cagcgtcagc
     6721 acaatgcgga caaggatgtg accatcggtc tggtgggcaa gtatgtggca ctccaggatg
     6781 cgtatctgtc cgtggcagag gcactgcatc acggcggcat cgtcaatgat gccagagtca
     6841 agatcctgtg gatcgactcc gagaagatca cacgggagac tgcaccggaa atgctgaagg
     6901 aatgcgacgg catcatcgtt cccggcggtt tcggcgaccg tggcatcgag ggcatgatcg
     6961 aggcgatccg ctatgcacgt ctgaacaagg tgccgatgtt cggcatctgc ctgggtatgc
     7021 agatggcagt ggtagaattc gcacgcaacg tggctggatt cccggatgcc aactcctctg
     7081 agttcgagcc ggacggcgca cacagcgtca tccacatcat ggaagatcag aaggacatcg
     7141 agaacatggg cggcaccatg cgtctggggc tgtatccctg caagctgaca ccgggcagcc
     7201 tggtacagca gatctacggc gaagagctga tctacgagcg ccaccgccac cgtttcgaat
     7261 tcaacaacaa tttccgcaag cagctcaccg aaaagggctt gcagatcgca ggactgtctc
     7321 cggacgagcg tctggtagag atcgtttccc tgccggatca cccgtggttc atcggcgtac
     7381 agttccatcc ggaattcaag tcccgtccga ataagccgca gaagctgttc gcatccttta
     7441 tcggcgcatc gctgaaaaac cggaacaaga agtaaaaata cagtattgcc ttgacatctc
     7501 agggaggtgt gattcatgag caaaatgctg atcgtagacg acgaagagaa gatccgtgcc
     7561 gtggtgcggg aatacgcaga gtttgaggac tttgaggtga ccgaggcacg ggacggcatg
     7621 gaggcggttg cactctgccg ggaaaatgac tatgatatca ttatcatgga cgtgatgatg
     7681 ccccggctgg acggctattc cgcctgcaag gagatccgga aacagaagag cattccgatc
     7741 attatgcttt ctgcccgtgg agaagaatac gacaagctgt tcggctttga gaccggcatc
     7801 gacgactatg tggtgaaacc cttttcgccg agggaattga tggcacgggt gcgtgctgtt
     7861 ctggcacgga gccatgcggc acagaagaac ggcgccgatg cgccccagca gcgtctgaaa
     7921 ttcggcggtc tggagatcga ccttgccgga cgggaagtct atgtggacgg cgtgcgtgcc
     7981 tccatgacac cgaaagagta tgatctgctg ttttatctgg tgaaaaataa gggactggca
     8041 ctctccagag acaagcttct ggaagaggtc tggggatatg atttcttcgg ggacgaccgt
     8101 actgtggata cccacatcaa gatgctgcgc ggcagccttg gaccgtatcg cacagatatt
     8161 gtgaccttga gaggaatggg atataagttt gatccggaaa atgacgcatc atccgcatca
     8221 ccggctcggt gagcgaaatc ttcgcatcgg cgtaaaaatc tggctgtttt tcatgctgtt
     8281 cgtgtgcgtg gtctttctgc tcatgtggct gttccaggtc atctttctgg aatggttcta
     8341 tgagtccatg aaaatccggg acaccgcaaa gctggcacag cagctggtct ctgactatgg
     8401 cagcgatgac ttttcgcagg atgcccagga aatttctctg caaaacgaaa tgtgcattga
     8461 actgctcaac acaaacggca gagaagtcta ttataattgt gtatacaacg gcaagtgcct
     8521 gctgcacggc gagggaaacg ggacattttt ctatctcatc gacctccaga acagcagcac
     8581 cggcacgatc tgccgcaagg tctccaaccc gaatctgcaa aatcagatgc tggtctacgg
     8641 ctgcaccatg tacagcaagg atgacggcag cgtgctgggc tatctgctgc tgaattcgcc
     8701 gctggtgccg gtggagtcta ctgtttccat tctgaaacgc cagaccatga tgattaccat
     8761 tatgctggta ttcttcggct ttctcatttc gttctatgcg gcgaaccaca tcgccacgcc
     8821 catcacacgc atcaccaaat cggcaaagcg tctggcgcac ggcgattacg cggtgaagtt
     8881 cgaaggcggc ggctatgcgg aggtagacga tctggcggag acgctgacct atgcggcgca
     8941 cgagctgtcc aagacggatc agatgcagcg tgatctgatc gccaatgttt cccacgacct
     9001 gcggacaccg ctgaccatga tgaaagcgta tgcggagatg atccgtgacc tttcgggaaa
     9061 caatccggtg aaacgggagc agcatctgaa tattatcatc gaggagaccg accggctgac
     9121 ccagctggtc aacgatatgc tggatctgtc aaagctggag agcggcgcac aaaagctgga
     9181 ctacagcacc ttcgacattg cccagtgcat ggaagagatc gtccaccggt atgagggcgt
     9241 gtcggagcgg atgggatatc acatccatct gacgctggac aagtcctgca tggtgcgctg
     9301 tgacgagagc aagatcgacc gtgtcatcgg caatctcatc aacaacgcca tcaactacac
     9361 gtccaaagag gacaagcagg tgtttgtgac ccagcggaat ctgccgcagt gcgtgcgcat
     9421 tgaggtgcgt gacaccggcg acggcatcga agaggacaag atcaagctga ttttcgacaa
     9481 gtattaccgt tcagagaacc acaagcgtga agtggtggga acgggactgg ggctgtccat
     9541 cgtcaaagag attttgaaga tgcacaacta caactacggc gtaaacagca agctgggcga
     9601 aggctcgacc ttctggttcg aaatccggga cattgtgccg ccggatacag cggaacagcc
     9661 ggaatcggaa atccagtgac tttttgaaga gaaagcggac agcgcagaac tgtccgcttt
     9721 ttttgttggt gtgattgcat ttgtatcggc ttaaaaggca caccgtaggg gcgaacatgg
     9781 ttcgcccggg actcttctga ctccgaccgc actgcgtgcc acctcctcta aaagaggagg
     9841 ctttgcgaaa ttgtctcctc tcatagagga gatgcccgac agggcagagg agtcttttgc
     9901 ctcccttgaa aagggagatg cccgataggg cagggggata tattatcttt gggtctcacg
     9961 ccccaaaccc gttcggcgga atcgctcgta cctcgctgtc cgcctttggc ttgcaaacgc
    10021 ttttgtttgc cttttcctgt acgtgcagtg taggctttct gggtttctct tgtgcaggtg
    10081 gtttttgttg ttttcatagc gtttgccacg caaattgtct ttgtatcgtt ttgaaagatt
    10141 caccgtaggg gcgaacattg ttcgcccgag actcctcagt caagcctacg gcttgccagc
    10201 tcctctgtag aggagccaaa agaaaagtct cctctcatag aggtgattcc ccacggggtg
    10261 gggaaatgtc accgtaggtg acaaaaggga ccgctcagta cgagatgccc gacagggcag
    10321 aggagtcttt tgccttcttc aaaaaggtga atctgtaggg ggcgatgccc agcctgtcgc
    10381 ccagcggaaa tggcgacggc agacttgcca aaggatagca agccacatcg ccccgttccc
    10441 tttctgaaaa aaggtttacc gtaggggcga acatggttcg cccgggactc ctctgactcc
    10501 tccggcactg cgtgccacct cctctaaaag aggaggcttt atcaaatcgt ctcctctcat
    10561 agaggagatg cccgacaggg cagaggagtt gtttttgcct tctttgaaaa ggtgaatctg
    10621 tagggtgcga cgggctgacc cgccgcaccg caatcaccta ctgaatttgt cgcatttgac
    10681 aaaaaagaaa tcttgttgta taataaaaca atcacatttt tctgcaactg cggagacaac
    10741 aaaggagaac tgttatgagt acatcaacca aaaaccgagc cacgttccat tcacggctgg
    10801 gctttatcct ggtatctgcc gggtgcgccg ttggtctggg caatgtctgg aaatttccct
    10861 atatctgcgg cgaaaacggc ggcggcgctt ttcttatcat ttacctgctg tgcctgcttt
    10921 tgctgggact gcccatcgtc atctgtgaat tcgccatcgg ccgtggcagc cgccggagta
    10981 tctcccaggc gctgaatatc ctggaacagc cgggcagccg ctggcatatc accaaatacg
    11041 ccggcatcgc cggaaactat ctgctcatga tgttctacac catggtcgcc ggctggatgc
    11101 tctactatgc gttcctgtac gggtcgggaa agatcacggc ggtttctgcc gaagagacct
    11161 ccggcttttt ctcccggatg ctggataatc cggggctgat gatcctcttt gcagcaattg
    11221 tcattgtcct ctgcatcggt atctgcgccc tgggtctcca aaacggcatc gaaaagatca
    11281 ccaaagtcat gatgatgcta cttattgtgc tgatgctggt gcttgtggtg aactcgctcc
    11341 ggctgaaagg cgcaggcgaa ggtctgaaat atttcctcgt ccccagcttt tcccgtctga
    11401 aagaaaacgg attcggctct gtggcattcg ccgctatgac ccacgctttc tttaccctga
    11461 gcgtgggcat cggtgcgatg gagatcttcg gcagctatct gaaaaaggga cggcgcatca
    11521 ccggcgaggc gatcaacgtt gtggtactgg atacctgcgt tgccatcatg gctggcctca
    11581 tcatcattcc ggcgtgcttt tcctatggcg tccagccgga tgccggcccg tccctgctgt
    11641 ttatcaccct gcccaatgtg ttccatcata tggtgggcgg acgcatctgg ggctgctgtt
    11701 tcttcgtgtt catgtctttt gcggcgcttt ccaccgtcat tgccgtgttt gaaaacatca
    11761 tcaccatgac cgtagaactg ggcagctgga cccggaaaaa gtccctgctg gtgaacctgg
    11821 tgttgatctt cgtgctgtct ctgccggcga tcctgggctt taacctgctg tccggattcc
    11881 agcctttcgg ggatggttcc agcgtaatgg atctggagga ctttttggtt tcgtacaaca
    11941 tcctgccgct gggttctctg gtgtatgtgc tgttctgcgt ccggaaaaac ggctggggct
    12001 gggaaaaatt cctggcggag gtcaacgagg gaaccggcct gaaactgccg ggctggctgc
    12061 gaggctatat ggcgtatgtc gtgccgacgc tgatttgtct gatctacctc aagggctatt
    12121 acgatatgtt tgccggaaag ggaacgggaa cgcttatcgg ctggatggcg ttcgctgtgc
    12181 tgctcctggc ggtgatcttc tgggtgtgct tttctgtggg gaaaaagcct gcagcaaagg
    12241 cggaaaccaa aagcaagtga cctgcggctg acatagtatc agtctcatac aaacaaaaaa
    12301 ccgtttcgct gtcggagacc ccggcgtgcg aaacggtttt cgttttattt agtgcattgc
    12361 cttgtttact ttcttctgcc acgctttgcg gtggtggtcg gttttgctgc ggcagccggc
    12421 tttgcagcgg ttgtctttgc agttgttgca gcagccttta ccggctcttc ctttttcaca
    12481 gcggtttcaa ccggcttgac agccttttct gctgcggcag gcgcagcctt cggtgcagcg
    12541 gctttgggtt ccgtctttgc cggttctgcc ttctttgcag cggcagtctt cggagcctca
    12601 gccattttcg gtgcagcctt ggcagcggtt ttctttgccg gagcctttgc cttcggtgca
    12661 gcagcctttt tcggtgtcgg cagggttgcc agaacttcac ggaatacagc caccttgtcc
    12721 cagtcgccct ccacggtcag agcgtctgtc tctgcgctgc gcagtgcgtc cagcagctct
    12781 gcggcgtcag cggataccag tacatcgtga cccttatagt catacggttc aatggtcagc
    12841 ttgtcgctgt ctgcggaaac tacggcatag aatgcgccgc agccctcacc ggaaacgtcg
    12901 aactgtactg caacggcagt gtccgcattc ttggcgctgg cgttttccag cattgttttt
    12961 gcctgtgcaa acatctcttc aaaagtcatt ttctgtcatt cctttcgctc catccggcaa
    13021 aaatacgccg gaatctcata tgcttttcct ctttattata ggcgtaaatc ggggaaatgt
    13081 caaggagaat gcagataaaa cgtgaaaaat aagcaaatat attgcataac cagaaaaagt
    13141 gcggtttcaa atttgtgcag tttcacgaaa gaaaaagctt gacaagccgg tgaaaatagg
    13201 gtatacttgt aaagcagatt acttttacag gcaggaggcg gcatggaaat gtccaaggat
    13261 ttgcgatttg ccatgctgtt ggactgctac ggcgaatttc tgactccgca ccagtaccgg
    13321 ctcacagagc tgtactattg tgcagacctg tccctcagcg agatcgctga gatcgtgggc
    13381 atcacccgtc agggcgtccg ggacggcatc aagcgtgggg agcagatcct gcaggagatg
    13441 gaagaaaagc tgcagtttgc ggcaaggctg aatgcgctgc gggaaaacta cagcgccatt
    13501 gccgggtcgc tggagcgcat ccaggcacag gcggcagatg taccggcggt gcgggacgag
    13561 tgccgccggg caatggaaag cgtccgccac gggctggagc tgctttgaaa aaagacgaag
    13621 gaactgcatt ttgtaaggct ctccttgaaa aaggcggatg ccggcgaaag ccggcagagg
    13681 gttatccctc cggcactgcg tgccacctcc cttttcaagg gaggcaatac aattgttttt
    13741 ctttgagagg aggagatcat tatggcattt gagggactgt cgggaaagct gaaccaggtg
    13801 ttcaaaaagc tgaaatccca cgggaagctg acggaaagcg atgtcaaaga ggcgatgcgt
    13861 gaagtccgca tggcactgct ggaggcggat gtcagctata aggtggtcaa ggattttgtc
    13921 aagaaggtct ccgaccgtgc agtgggcgaa gaggtcctca ccagcctcac cccggcacag
    13981 caggtcatca agatcgtcaa cgaggaactg tgcaatctga tgggcaatga aaatgcccgg
    14041 atccagttcc cgtccaagcc gccgtgcatc attatgatgt gcggtctcca gggttccggt
    14101 aagaccaccc acgccgcaaa gctggcgaag atgctgaaaa aagaagggca ttatccgctg
    14161 ctggtggcgt gcgatatcta ccgtccggcg gcgatcaatc agctgcaggt ggtgggtgag
    14221 cgtgccggtg tcaaggtctt tgagatggga cagatcgacc cacgcatcat cgccaaggag
    14281 gcgctgaagt tcgccaagga tcacggcaat gacgtggtca tcctggatac cgccggccgt
    14341 ctgcacattg acgttgcgct gatggatgag ctgaaagatc tgaaggaact gacccacccc
    14401 aacgagatca tgctggtggt ggacgccatg accggtcagg atgccgtcaa tgtggcaaag
    14461 gcgttcgacg atgccctggg catcgacagc gtgctgatgt ccaagctgga ctctgacacc
    14521 cgtggcggtg cggcgctgtc cgtgctggct gtcaccggca agcccatcaa atatgtgggc
    14581 atgggcgaaa agctggacga gttcgaacag ttccatcccc agcggatggc atcccgtatc
    14641 ctgggcatgg gcgatatgct gacgctgatc gaaaaggcgg aaagcaccat cagccagaag
    14701 gatgccgaga agatggcgaa gaagttcgag gaaaaccggt tcgatatgaa cgatctcctg
    14761 gaacagctgc gtcagattca gaagatgggt tcgctcaagt ccatcatcgg tatgctcccc
    14821 ggcgtgggca gcaagctgaa agatgcagac attgacgacc ggcagttcgt ccgcatcgag
    14881 gcgatgatta cctcgatgac caaggcggag cgtgcaaagc cctcgatcat caacccgtcc
    14941 cgcaagcggc gtatcgctgc cggcagcggc acgaaggtgg aggatgtgaa ccgtctgctg
    15001 aaacagttcg accagatgca gaagatgatg aagcagttcg gcggcaaggc aggcaagggc
    15061 ggaaagatga accgccgtgc catgaaacgc ctggcaaaca tgaatcccga acagctgcaa
    15121 agtttgcagg gtggcatccc gggcgttccg ccggggctga tgccgccgaa gaagtaagag
    15181 atcccgcagg aaactgctgg agatagattt gacatttgtc aagaataata catggaggta
    15241 aattcaaatg gcagtaaaga tcagactgag aagaatgggc gcaaagaagg ctccgttcta
    15301 tcgtattgtt gttgctgacg agcgttatcc gagagacggc cgtttcatcg aggagatcgg
    15361 tttctacgat ccgaccaagg aaccgaccgt agttagcctg gacgctgaca aggcaaagac
    15421 ctggctgaag aacggcgcac agccgactga taccgttaag gttatcctga agaaggaagg
    15481 cgtactctaa tttcttagag gcaacaccga gtgcggggtc gttctgcaaa ggcagaacgg
    15541 gattgctccg ccggtgaacc gtatcggcgc tgtccggctg gacggtgcgt gtgctgcaca
    15601 acgggtacgg ctgaccagtg gagcaatcat gtgaccatag aaggaggaaa agatcatgca
    15661 ggaactgttg cgagtgatcg ttgccggtct ggtagaagat ccgacagcca ttcagatcac
    15721 ggaagatgcc cccaatgaag agggcgtgat cgtttatcat ctgcacgttg caccggatga
    15781 taccgggcgt gtcatcggaa agcaggggcg cattgccaag gcgattcgtg tcatcatgcg
    15841 ttccggcgca gcaaagtata atcagaagat cgctgtcgag atcgactgag gaacagctac
    15901 aaacaatcca tgcaggcgga cagaaatgtt cgcctgttct tgtatgtaca gaaaggactg
    15961 ctcatgaaac aataccttga aatcggaaag atcgtcaacg tccacggact tcgtggcgac
    16021 atgaaagtcg tgccgtggtg cgacgatccg gaattcctgt gcgaattcga taccctgtac
    16081 ctgggcaagg cgcaaaagcc ggtgacggtg actgccgcac gcctgcaaaa gggcaacgtg
    16141 ctgctgcatc tggagggcgt ggacaccgtg gaggaggcag aaaagctgcg gaatcaggtg
    16201 ctgtatatgg accgggacga agtggaactg gaagagggcg tgtacttcat ccaggatctc
    16261 atcgggctgg aagtgctgga cgtggacacg ggcaaaagct acggcaagct caccgatgtg
    16321 atgcagaccg gcgccaatga cgtgtacgag gtcaaagatg cggacggaaa gaccgtgctg
    16381 atcccggcga tcccggatgt ggtgcaggag accgacctgg acggcggcgt aatgcgcatt
    16441 cgtccgctgg agggcttgtt cgatgcagat tgagatcgct accctgtttc cggaaatgtg
    16501 tgaggcggtg ctgtccgaga gcatcatcgg cagagccaga aaagccgggc atatctctgt
    16561 ccgctgctgg aatatccggg actataccct ggacaagcac cgccgtgtgg atgatgtgcc
    16621 ttacggcggc ggcagaggca tggtcatgca ggcagacccc atctaccgct gctatgaggc
    16681 ggtctgccgg gatatcggca caaagcccta tgtgatctat atgtccccca agggaaaggt
    16741 gctggaacag cagcgctcgc tggaattgtc cgtgatgccg catattttcg tgctttgcgg
    16801 gcattatgaa ggtgtagacc agcgcatcct ggacgagatc gtggacgagg aggtctccat
    16861 cggggactat gtcctcaccg gcggcgagct gccggcgctg gtgctggtgg ataccattgc
    16921 acggatggtg ccgggcgttc tgccggaaaa cctgtgcttt gaagaggaga gccatttcgg
    16981 cggactgctg gaatatcccc agtatacccg tccggcggtg tggcacgacc gggaggtgcc
    17041 gccggtgctg ctcaccggac accacaaaaa cattgcagaa tggcgcagcg cacagagtct
    17101 ggaagagaca aaaaacaagc gccccgacct ttatgcaagc tatttggata aaaaataact
    17161 gttttctttg tggaatttga acaaaatatg caacttcgga aaaaggggaa aattccattt
    17221 tgacattgtt gtcatgttcc acaaagattc aaacgaaaag ggagaacggt tataacaaca
    17281 cgtagaaaat tccggaaaat cggacaattt ctgcaaaaaa tgcgttgacg tgttccggaa
    17341 aaaacggtat aatggtagca tagaagggaa gaggaatgca caaattgttt ccaggggaat
    17401 ggaaaccgca aagcttcttc aaaccaacgt atgatgcgtg aaagcgcagg atcataagga
    17461 tgggagagtt tttatcatgt ttgtaaaaga agttacagta cagaatcagg ttggtctgca
    17521 cgcaagaccg gctacatttt tcattcagcg tgccaacgag ttcaagtcct cgatctggat
    17581 cgaaaaggaa gagcgtcggg taaatgcaaa gagcctgctg ggtatcctgt ctctgggtat
    17641 cgtaggcggc actgacatca agatcattgc ggatggttct gatgagcaga tcgcagtaaa
    17701 ctctctggta gatctggtag agagcggttt cagcgactaa ttcgctgttg tttcgacaag
    17761 agaacctcac gggggagcaa gttccgccgt gaggtttttt gtttttgcgc cggcttagct
    17821 gccgctcagc ccatagcgca ccatattttc acagaaaatg ccgtagcccc gtgtggggtc
    17881 gtccacaaat acatgagtca ctgcgcccac cagtctgccg ttttgcaaaa tggggctgcc
    17941 gctcatgccc tggacgatgc cgccggtgat ctccagcagt tccgggtctg tgacgcatac
    18001 ggtcatgttc cgggtgctgt ccgtgccgct gtagtcgatc tcttccagca tcacgtcata
    18061 tgccttcggc gtgtcgccgg agacagtgca gaggatctgt gccggaccgg tctcgatctc
    18121 ctgtttcagc gccatgggaa cggcgtcctc agcggatgga ttgtggtaca gcgtcccgaa
    18181 cacgccgcag cggttgttgc agatcagctc gcccagggat gtctcgtccg aaaagctgcc
    18241 ctggagcatt cccggactgc cggagacgcc tttcaccacc tgggagatgg tcacagcagc
    18301 tgcctcgccc gaaccaaagg gcaggatgtc gccggtgtcg gcatcgcaga tgggatgccc
    18361 cagaccgccg aaaccgccgg tttccggctc ataaaaggtg atggtgccga tgccggcgct
    18421 gctgtcccgc acccagatgc ctgcctcata gcggttgtct gcggcggcat atgccggcgt
    18481 cagtgtcacc gtgcaggtct cttcgccccg ttccagcgtc actgtaacgg gaacaccgtc
    18541 cgaatcggcg atggcgtcct ggagcgactg gttggagtcg atgacctgtc cgttcacaga
    18601 acggatgagg tcgccggtct ggatgcctgc ctcctgtgcc ggacagcgcc tgccctccgg
    18661 agtgtccacc cgttccatgc ccaccaccat agcgccgtcc atgcggagct tgatgccgaa
    18721 cggctcgccg caggggacga gcatgggcgt gtcgatctcc tggacttcca cctccttgac
    18781 gggaatgacg ccgaagagcc ggagggatgt ggtctcggca gtgcgtgccg atgcggctgc
    18841 cgcctggacg gtgtgcgccg tgggaacggc agagatatgg agcgccgtgt cgacttccag
    18901 ctggctgccg gtctgtacat aataattgtc cggcagctgc caggcgtaat atgccgcagc
    18961 gccgtataac tgcagcagca cggcagcacc gagaactgcc gtgcgcttga tccaacgtat
    19021 catttttgta tcctgccttt ctggtatatt tattttttgc cccgtatcta tcgtgtgcag
    19081 tacggcagcg cttatcagcg acaatgcaag gaaaaaacat agggcagttg ttctcacaac
    19141 aggcagcaac tgcctttccg aaaaacggga gaaccacttc cgcaattcca aaacatccaa
    19201 aaatttgaaa atgaaaggat ctaccgccat gaaaaccaaa aacgccgcct ctcgtcccat
    19261 tcccaccgcc gcacagctga aaaaacagcg tgtccgccgg gtgagctgta tcatcctccg
    19321 catcctgctg gtgctttcca ccatcctgtt tacctggatc acctgctggg gctgcggact
    19381 gggctggatc agccgtgcca gagccggcag caactggccc atgtcctttg tgggctacgg
    19441 gcagatgctg ctggtgggtt cgggtctgct gactctgggg acagtgcttg tcctgctgtg
    19501 ccggaagaac tggctcaact gggcggcggc aggcagtgcg gcaggcggcg tggtgctgtg
    19561 cctgctggcg ctgtaccatg tggcatccta tgcgaaggag cattcctttt acagccagct
    19621 gatgcagatg ccggcggcga cgctgtactg cgtacagatc ctgccggtga tcctgccctt
    19681 tgtctgcgtt acggcgctgg gactgttaca gttcttttcg cctgaggcgg tacagcgccg
    19741 ccgggacaga aaactggcgg attcggcaaa ggcgccgtcc attctctgag tgtccggaac
    19801 gtcctgtatg gttcttctct gctttttaca cagattttac aaagataatt tctttttcga
    19861 caagtttcag ccttgcagtt ctccaaagaa tgtggtaaaa ttatagggta gaacaaaaaa
    19921 acaggaggta acaaaatgga caacaaaaaa ataaaagtcc tgatcggaga cgacagcgcc
    19981 gagtatggca taacctgtgc taacgaactg cgtgcgctgg gtctttacgt cgtgacgaga
    20041 cgcaaagacg gcaaggtcat tctggatgcg atccggaacg acacgccgga cgttgtggta
    20101 atggacgcag tgctgcctgg catggatgcc atagagatcc tcgagaaatc tcagacattc
    20161 gctcatgcgc cggctttcat tatcacgtca tcttatgaga acacatttat tgagcgccag
    20221 gtcatggaaa agggtgcggc gtatttcatg ctgaagccgt ttcacatttc ggcgctggga
    20281 aaccgcatca cagaactggc atccaacggc attcccgaca aagcggcatc tgcgcaccg
//
LOCUS       DAWENO010000019        20029 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1128_length_20029_cov_10.501602, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000019 DAWENO010000000
VERSION     DAWENO010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20029
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1128_length_20029_cov_10.501602"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1289..1298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10702..10711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4494 a         5524 c         5225 g         4766 t
ORIGIN      
        1 cccttatatg ccacgtagac cattgcggcg aagatgtcgc tggtgtcgat cttgccgtcg
       61 ttgtccaggt cacccttggg atatgccgtt ttttctaccg ggagactttc cccgtagtgc
      121 aggactgcgc cggtcagagc gtcgttgcag aatcccacag agaccatgtc ccacgcctcc
      181 tgggtgccgc cgaaatatac gtccgtcagc gcatcgcact ggtcaaaggc gtattcgccc
      241 acgctggtga tgccctccgg gagctgtacc ttttccaggc tggtacagcc ccagaacagg
      301 tactcgccga tctccgtgat gcctgccgga acaaccgctt tcgtcagcgc tgaacagccg
      361 gaaaagacgc tgccgcccag gctggtcaca ctgtccggaa cggatacgct ggtcagtttc
      421 gggcagctgg aaaaggcgga gtcgccgata atggtgacgc tgtccgggat ggtgacgccg
      481 gtcagctccg tgcagccgaa aaaggcagag ccgctgacga tcttcaccgt gttcgggatc
      541 gtcacattgc cggaacagcc ctcgccgttc agcaggatgg cattcacaat gaccatggga
      601 ttttctgcct tcttcgcctc catccaggcg gtattttgaa aggcattttc ccggatggcg
      661 gtgacgctct tcggaatgga aatggttttc agattggagc agttatagaa cgcctggatg
      721 ccaatgtctg ccacgccgtc cggaatggtg acttccgtca gcttggcgca gccgataaac
      781 gtccagtttt ccagcttttt cagctgtttc ggcaggctga ccgatttcag accagtgcag
      841 ttggcgaaag cgctgttgcc gatggcggta acgctctccg gaatggtgac gctgaccaga
      901 ttgtcacagc cggaaaaggc agaattgccg atactggtca cgccgtccgg gatggtgata
      961 ccggtcagcc cggtgcagtc cgcaaaggcg gagctttcaa tgctggtcac gccgttggga
     1021 atgatgatgt ttccggaaca gttggcgccg tccaccagct tgttgttgat gatgaccagt
     1081 gtgctgccct cctgtttcag cgtcagccag ggcgtgtcct ggaacgcctt ttcaccgatg
     1141 gtctccacgc tctccggaat ggaaatcttc ttcaagctgg tgcagccgtt aaaggcacgg
     1201 tcgccgatct cggtcacgcc gtccgaaatc acaatggcag tgagtgcatc acagtacgca
     1261 aaggcagaac tgccgatgct ggtgacgcnn nnnnnnnntt tgcaggttgg tgcagttgtt
     1321 gaaaacgccg ctttccagcc gggtgatgcc gttcataccg gtcacttcgg tcagagaagt
     1381 gcagacggaa aatgcccagg tgccgatctc ggtgacgctg tccgggatgt gaagcgtttt
     1441 cagcgcagtg cagttgctga acgcagaacc gccgatatga gtgacgttct ccggaatggt
     1501 gactgccgcc aggctggtgc agttggaaaa agcggaattg ccaatgctgg tgacgctctc
     1561 cggaatggtg acgctttgca agttctcgca gttgtagaac gcataggctg caatattggt
     1621 gacaccctcc ggaataacca tttccgtcag actggtgcag ctgttgaaag cctcattccc
     1681 aatggaagtg acctggctgg gcatggtgca gctttgcagg ctggtgcagt gggcaaaggc
     1741 aaaagcgccg atctgggtga cgctgtccgg gatgtccacc gtttccaggg cgtagcagtt
     1801 atagaacgct gcgccgtcaa tgacttccag agtggacggc aacttgacct ctttcagcga
     1861 ggtacagccc aggaaaaagc cggaatagga atcatcgtcc ggataatact gcgccgccag
     1921 cgacgtgatg ccctccggga tcgcaatggt ttccagagcg gtacagctgt tgaaaaccgc
     1981 cttgcccata gaagtcatct gcgccggaaa cgtaactgtc gtcagctggg tacaggagag
     2041 aaatgcgcag ttgcccaccc gggtgacgct gtccggcagc tgtagactgg tcagtccctc
     2101 acagcagccg aacgcatagt ccttgatctc cgtgacaccc tccgggatct ggagagactc
     2161 cagagcggta cagctgtaga acgccgcctc gtcaatggtt ttcaaggtag atggcagctg
     2221 cacttctttc agtgaggtgc agaacgcaag gaaaccgtac cagctgtctg ccatttcggt
     2281 atccgccatc accggcagag atgtgatgcc ctctggaatg gtgatcttct ccagcgaggt
     2341 acaggtgttg aacacttccc tgccgaacga tgtcatcgtc tggggcaggt caatatgcgt
     2401 gagagcggta cagccggcaa aggcagaatc gccgatactg gtgacgctgt ccggcacgga
     2461 aacgcctgta atggcggtgt tgccctggaa tgcgctgtcc ccgatccggg tgacggattt
     2521 tccgccgatc tccgccggaa gttccagctc accggagaca tccgcagaac agccggtaat
     2581 ggtgactgtg ccgtcctcct ctgtcgtata ggtcagattt tcgtatgtac tctccacagc
     2641 actcaccgaa agcaccgcct ccggcagact cgccgcagac agcggcactg cacccataca
     2701 cagtacccct gctgtcagag ccgccaggat ttttttcatt ttacccaatt tcattcgctc
     2761 ccttcatagc tttttgttac cattttacag tataatatga catagtatca taccacatta
     2821 tttattataa cacagaaaag tcgaaagcga tacggagatt ctcagaattt tccattaaca
     2881 aaagcgatgc cgcctgcaaa aaaggcacgg tgtctgcgca ccatgccttt cccttgtcta
     2941 taggatattc caccaaaacg gcatctcact ttcgctcagc cgctggatcc acattcgagc
     3001 ctctgagagt tcggagtcca gctcgtcaca gtctgcgccc agcattgggg tcagatgggc
     3061 aacggtcacg tcgatctgca ccaccttgtc ccgccacacc accagattga ggcggcgttc
     3121 ggacttttcc cactggtcgt ggaactgctg gaatgcctgc tctgcctgtt cgggagattg
     3181 cagggcagtc tcccggagct ggtccaggtc gttcagcagg cggctggtct ccgagcgcac
     3241 cagccacgcc gacgcactgc tgagcagtac cagcaccagc agcactgcta tgccaagtct
     3301 gacacgattc atgcgacatc cccctgtttc ttcggtatca cggtacattt tccgtcggca
     3361 tcactgagca tgaggaacac ctcgctttct ctgagccgat gctccgccag cttttgttcc
     3421 agccacgcct ggtttttccc cagcatcgcc agggatttgt tcatgacaca gccgtcctgt
     3481 atcacagcca ccggaggatc ctgggctgct tttttcagtg caacgtcctc cggcgtcagc
     3541 ggctgacacg ccgccttgag gtagacggag actgttcccg tggtttccat cacggcgaac
     3601 tgcacctggc tgatgtcgaa cacctgctgc tggcgaagcg ccgtcatcag atcgtccacc
     3661 gacagccgca ggtcacgcat ggtcgcctgt tcgatctgac cgtttcggac gatgatcttc
     3721 ggccgcccgg agatgatgcg ccgcatccgc agggatttca gcgtcaccca ggagatcacc
     3781 acctcaaagc acaccagggt gaggatgggc aggatgccca gaaccagcgg gatgctggta
     3841 tcctccaggg gcagggtggc gatgttggag atcagaatgg tgatgaccag ctctgagggc
     3901 tgcagttccc ccagctggcg cttgcccatc aggcgcacgc cgaagatcac cacaatgtag
     3961 aggatcagcg aacggatcag aaccacactc atgaaaaaag cgcctccttt gcatgacagc
     4021 atcacacaga gcctgtgcag tgctgccaat catatctgtc acaaggacag tatgcgcaga
     4081 gacgccgggg attatacaaa aaagacatcg ttattccgtc ttttcctttt tcttgaaaat
     4141 gaccagtttg ctgaaaatgt agttcagcac caggatcacc acctgtgcca cgacctttac
     4201 caccagagag cgccatgtga cgatctcgcc gttgacggtg gcgaagaagt ggttgccgcc
     4261 ttccataatg gcaagctcca ggaaataggt caccgcacgg gcgccgtaga acatatacag
     4321 ttcccgccag aacgccgcac gggtatcggt cacgctctgg aacacatact tcttgttggt
     4381 gaaaaaggca aaggtcacgg cgaagatcca cgaaatggtg gtggaaagcc cggtgttcag
     4441 ccagtttgcc atgtgcatcc gggtaaacag cgccgtgggc agcatctgaa agatcagaga
     4501 aacaatggtg gtcagtccgc ccaccagaat gtacatccac acggattcgt gggaatacca
     4561 caatttttga atgggtttcg gcatatggtc aatgatccag tgaattgctt ttaacataaa
     4621 aataacctct tttttgattc atttcgacag ttcctcttga ttctttctgt attttatggt
     4681 atgatggata agatagtact gcaccgcagt agaaaggaac cgctgcataa agcctgactg
     4741 acggctttgt gcggtgtcgc cctaaaattt cgattccatt gggaggaact attattttgg
     4801 tttacgtcat accgatactg attctcgtcg ccttttctgc attgttttcc agcacggaaa
     4861 cagctttcgc ctgtgtcaat aagatacggc taaagcacat ggcggattcc ggggacaaac
     4921 gtgccgaaaa ggcactggat atcgccaacc gctatgaaca ggcgctgacc gccattctga
     4981 tgggcaacaa cgtggtaaac atcggcgcat cctcgctggc aacgatcttc ttcaccgccc
     5041 acttcggctc tgccggcgca gccatttcca ccatcatcat gaccgttctg gtgctgacct
     5101 ttggtgaagt gctgccgaag tcctacgcca agtcccatgc ggacaagctg gcgctggcgc
     5161 tgggcggcat cctatccaag tttactctgc tgatgacgcc tttcgtactg ctgttcggcg
     5221 tactttcccg ggcattcact gccaaggagg atgcgcccag cgtcacggag gacgagctga
     5281 aatacatcat tgacgagatc gaggaagagg gcgttctgga agaacaggaa agcgatctgg
     5341 tgatctccgc cctgcaattt gatgagacct ccgtcagcga gatactgatt ccacgtgtcc
     5401 ggatggtcgc cctgtcgtcg gatgccacca tcgaagagac aaagcagatg tttctgaaca
     5461 atcacttttc ccggtttccg gtatacgaaa aggacatgga caacatcatt ggcatgatta
     5521 ccaacaagga ctttttccgg ctcctgtgcg gcgagtttca gacgctggca gagatcaccc
     5581 agaatgtgat ctacgtgccg gacaccaagc gcatcagcga cattctccgg gatatgcagc
     5641 acgccaagac acaccttgcc attgtggtag accagtacgg cggcacaaag ggcatggtca
     5701 ccctggaaga catcatcgaa cagctggtgg gcgagatcta cgacgagagc gacgaggtag
     5761 tgcatgaatt ccagaaaaca gcaccccaca cctacacggt ggtcggttcg ttcagcgtca
     5821 gcgacctgtg ttccgcactg gacaaggaag atgccccggc gaaagtgccg gagacggaat
     5881 gcactaccat cggcggctgg gtcatggaac tcctgggaca catccctgcc gcaggcgaga
     5941 ccgtctcgga cggcacgttc catttcaccg tactggaagt ggacaaccag accatctgct
     6001 ccatccggct ggaagtcaca ccgccggaga ctgaacatcc ggaagaaaag taatccattc
     6061 tctcaagcaa agcaggcagc cagaatccgt ttccggctgc ctgtctttag gcaacaccgc
     6121 acaaagcttt atgcggtgtt gcctttattt tttcttatat ttcagcaaga taaacgccac
     6181 cagtgcggtc aggaccagcg acagcacaga gggaaaccat gtatgttctg caaagggcag
     6241 atccagggtg ttcatgccgt aaaagctgta caccatcgtc ggaatcgcca ggataatggt
     6301 cacaatggtc agacgccaca taacaatatt cagattgttg gaaatgaccg aaccgaacgc
     6361 ctccacaacg ccggacaaaa tgcccgaata gatgctcgcc atctcctgcg cctgtttcag
     6421 ctcgatcagc acatcttcca gcagatcctg gtcctcttcg tagagtttca ccactctgcc
     6481 acgcagaatc ttgttcatgg tcacatcgtt ggatttcaga gatgtggaga agtagatcag
     6541 agacttttcc aactccagca tctgaatcag ttcgtggttt ttcattgcac cgtgcagttt
     6601 ccgctgcatg gcgctggaag tcttgtcgat ctgtttcaga tagaccagga acgatgcggt
     6661 aatgttcatc atcagccgca gcacaaactg tgtccggaac tgggtctgga tattccgcac
     6721 ccgtccgctg accacatcgt caatgacctt gttttctcgc agggaaatgg taaagacgta
     6781 gtggtcggta ataataatac ccagcggcat ggtgtagaag atcaaggtat cgtctttctg
     6841 cacctcagac accgccgtat ctacaatgat aagggtctgg ttttcctcct gttccacacg
     6901 ggacgactct tcttcatcga gtgaggattt cagaaagtcg ctgtccagcc cgtaatcatc
     6961 gatcagcatt tttcgttcct gtgccgtggg gtcaaccacg gagatccagc agcccggcat
     7021 cagagaatcc acctcaatga gccggtttgc cactgttttg taataagaga ccaaatcgga
     7081 taccaccttt ccgcaaggca gcccgaccgg gttaacggca tccgctgtcc ttgcctgtat
     7141 tccatgtgtt catccgtttg ccataagctt tttccgggca tggcaaggga taagggtcag
     7201 cgttcatgcc gcacctccta gttttatgat attccggcat ttttcaattt gccgcacttt
     7261 ttattataac agaaaaaacc gcccttggca aggcggtttt tgtaaaattt aagcgatttt
     7321 tgtgaaacaa tggtcagtgc ttttggatgt cctcccggat gcgttccttg atgccctcga
     7381 agatgacctg ttttgcatgg attgcctgtt cctttgtcgc cgccggaacg cctttcagcg
     7441 gatagtcgat ccccaggttt tcatacttga cctcacccat ggtgtgataa ggcagcacgt
     7501 ccagtgcctt cagattatcc agaccgccga taaagtgacc cagctgccgc agatagtcgt
     7561 cgttcagtgt gatgcccggc acgaccacgt ggcggatcca gaccggaacg cccttttcct
     7621 tgaggtactc ggcgaattcc aggatgtgcc tgttgctctg cttggtgagt ttctggtgtt
     7681 ctgcatcgtc aatgtgcttg atgtccagca tgaccagatc cgtcactgcc atgagccggt
     7741 cgaattccgg gcggcgttcc tgacggaaca gaatgccgga ggtgtccaga caggtattga
     7801 tacccttttt ctttgccgcc tcaaacagtt ccgttaagaa ctccatctgc accagaggtt
     7861 cgccgccggt tgcggtaatg ccgccgtcgt ggagaaattc cttaatgccg tcgtactctt
     7921 tcagcagaga ttccaccgtc cggagattct cctggttctg attgtcgatc gtccaggtgt
     7981 ccggattgtg acagtagaga cagcgcatgg gacagccctg gaaaaagatg acgaaccgga
     8041 ttccggggcc atcgactgtg ccgaaacttt ctgtggaatg aatgtgtcct tccatgttgt
     8101 tctcctttct tgaacgcagt gcaaacgaaa gcgttcggat acaacaatgg gcgcacagaa
     8161 actgcacgcc cactgctaac attatgctat gtttatttta cgattccagc acgtttctgc
     8221 ttacagagca gcgtggaaag ttctggtgat aacgtcatcc tgctgttcct tggtcagctt
     8281 gatgaagtta actgcatagc cgctgacacg gatggtcagc tgcggatact tttccggatg
     8341 agcctgtgcg tccagcagag tctcacggtt cagaacgtta acgttcagat gatgtccgcc
     8401 tttttctgca tagccgtcca gcagacctac cagattttca atttgttttt cgtttgccat
     8461 gataaattcc tccttgagaa tttacgaatt gggataaata ccggggacgg atgagactca
     8521 ttcgtcctct ataaactcag tcggctgaca gcaagccatg ttccctttcg agcaatccat
     8581 gctgcgatag gtctttacat tcaagccctc gcccatgagg tcgtcctcag agatggtgag
     8641 atggccgctg ccgtcttcca tgagcttttc gatcatgtcc accagttcat ccgacttgtc
     8701 ctgttctttg acttcatgtt ccgccatgac cctgcgcctc ctttcaaata gtccttttca
     8761 gacttactgc ttttccattt cctcacggat ggaagcaacg tcgatgtcgc ctgcgaatac
     8821 agcagaatcc ttacccagag catccgggat gatggtgaag gtattggaga taccatcctg
     8881 tgcattcttc cacggcagct tagcaacaga tgccatagat gctgctgcac cgtgggtgtc
     8941 acggccgtgc atcgggtttg caccaggagc cagcggctga cccttcttac ggccatccgg
     9001 agtgttaccg gtcttcttac cgtatacaac gttggaagtg atggtcagga tagaagtggt
     9061 cggaacgccg cctctgtagc agtagttgct gcggatcata tccatgaact tgtccaggat
     9121 ctcaactgca atgctgtcta cacggtcgtc gttgttgccg tacttcggga agtcgccttc
     9181 tacttcgaag tcagttacta cgccgaattc gtcacggatg cacttaacct ttgcatactt
     9241 gattgcagac agggagtcag ctacaacgga cataccagcg ataccagttg cgaaataacg
     9301 ctttacgtct ctgtcgtgca gagccatctg gattgcctca tagcagtact tgtcgtgcat
     9361 atagtggatg cagttcaggg tatctacgta cagcttagcc agccatgcca tcatgtctgt
     9421 gtactttgcc ataacgtcat cataatccag ataatcgcct tctaccggac gatacttcgg
     9481 accaacctgg ttgtgcttac cggtcttagc gtccagtctt tcatccacac cgccgttgat
     9541 tgcgtacagc aggcactttg ccaggtttgc acgagcgccg aagaactgca tttccttacc
     9601 aacacgcatg gaggatacgc agcaagcgat ggcatagtca tcaccgtgtg tcacacgcat
     9661 cagatcgtcg ttctcatact ggaaggaaga ggtgttgatg gacagctttg cacagtagtg
     9721 cttgaatgcc ttcggcagtc tcttggacca cagaacagtc aggttcggct ccggagcagt
     9781 acccaggttt tccagggtgt gcaggtaacg gaagctgttc ttggtaacca gagttctgcc
     9841 gtcgatgccc ataccgccga tagactcagt aacccactgc gggtcgccgg agaacagtgc
     9901 gttgtattcc ggagttcttg cgaacttgac catacgcagc ttcatgatga agtggtcgat
     9961 gtattcctgt gcctgttcct cggtcagagt accgttgtcc aggtcacgct gaatgtagat
    10021 gtccaggaat gtggaagtac ggcccaggct cattgctgcg ccgttctggt ccttaacaga
    10081 acccaggtat gcgaagtaag tccactggat tgcttcctta gcggtggtag cgggaccaga
    10141 gatgtcatag ccatacttct ttgccatgtt cttcagagcc ttcagagcct tgatctgttc
    10201 tgccagttct tcacgcagac ggatcttctc ttcggtcatg gtgccggaag tgatagcctt
    10261 ctgatagttc ttgtcctcga tcagacggtc tacaccgtac agagcaacac ggcggtagtc
    10321 accgatgata cggccacggc cgtaagcatc cggcagaccg gtgatgattg cattgtgacg
    10381 agcagcacgc atttccggtg tgtaaacatc gaatacgcct tcgttatgtg tctttctgta
    10441 gtgtgtgaag atatagctga tctccgggtc tacttcatag ccgtaagcct tgcaagcctc
    10501 ttcagccata cggataccgc cgaacggctg cagggagcgc ttcagcggct tgtcagtctg
    10561 cagaccaacg atggtctcca gatccttatc gatgtaacct gcatcgtgag aatctactgt
    10621 agaaaccagc ttggtgtcca tgtccagaac gccgccgttt tcacgttcgg ctttctgcaa
    10681 ttcctgcaat ctgccccaaa gnnnnnnnnn naatttccac gctgtttgca caccgctttt
    10741 tttgccgtca gaggactgtg ttttgatctg tcttaataat atcacatctc aactatgatg
    10801 tcaataggtt tcttgcaatt tcccaagatt tttttttgca aaaccttatt ttttgccgat
    10861 ttgctcccat gcgccgaacg gttttcagaa taaagaaaag aaaaatcaca tctcctgttt
    10921 tttttgttaa tttgtaggta caaattcggc tttgacggtg acagaatgac gtgctatact
    10981 aaaggcggcg ccgcacacac accgatcggc aagaacggag gtctttttta tgttacagtt
    11041 ttcaccaaac agcaccatcg cactggttgg cagttccgac gggctgcccc ggggcagtga
    11101 cgccgttcca cggctctgcg cttttctgga atcgctggga ctgcgcacgg tggttttccc
    11161 gtccttgtac cagactgccg ccggcacacc ccagccggca gagcgacgtg ccgcagacat
    11221 ccaggtggcg tttgcgtcgc cggaggtgga tgcagtcttt gatctgaccg gcggcgacgg
    11281 ggcaaatgta gttctgcccc atctggatct ccggaagatc gcccacactc ccaagccctt
    11341 tttcggatac agcgacgttt cggctctgct caatcccctg caggatttcg gtcacgtgcc
    11401 ggtgttctac tgccagccac gctttctggc aagctgccct gccgccgcag aacggttcac
    11461 acgctggata cagcggcagg acgacctgtt ttgctttcgc tgtacctttt tacagggcag
    11521 ccagctctcc ggaaaagtct gcggcggcaa catccgctgc acgctgaaac ttgccggaac
    11581 gccgtggcag ccggacccga cgggacgggt gctgttcctg gagagctgtt ccggcagtct
    11641 tgcccggatg gagaccatgc tgcaccagta ccggcaaatg ggaatgttct cccggtgcag
    11701 cggcgtactg ctgggaaatt tttcggagct ggcgaaaaag cagcagctgc cggaactgta
    11761 ccggatagtg caggacatca tcgccgaccc cgcactgccc cttgccgtga ccacggagct
    11821 ggggcatcag ccggatgccc ggtgtctggc gtatgcggaa atctgcctag aaaaaacgca
    11881 gctgtagcca gcccactgcc agcaaaaccg ccagcagtcc gaagttcacg gcagagaaaa
    11941 tgcccaggta ttttcccaga cgggaatgcg ccgccttgct gtaaaactga taggagatca
    12001 gactcgccag agaggcgata ggcgttccca gaccgcccag gttcacgccc agcagcagcg
    12061 cctttgcatt ttccgtaaag ccggacagca tcacagccgc cggcacattg ctgatgcact
    12121 gtgaaagcgc cgccgagacc cacaactccc tgccctgcat cacgccggca aagaacgccc
    12181 ggacggcatc gacccgtgcg atattgccca caaacacgaa aaagcagacg aacgtcccca
    12241 acaggcagta gtccgcctgg gcgaaaagcc ttttgtcaca gaaaaacacc agcacggcag
    12301 tcagcacaag gcagagccag tccgggatca cccggaacac ggtaagcaga cacaccagga
    12361 acagcagcag atacaccgcc gccggctttt tcgggatgac aggcaaggtt gtttccccag
    12421 gttttccggc acagggtgtt ttcggcagca ggaacgacaa tcccagaagc accagccagc
    12481 tcagcacgcc ggcagggagc atggtacgga aaaagtcgcc ggtgtgcagc tggtacgccg
    12541 catagaggta caggttctgg ggattgccca ccggcgtcat catactgccc agattcgcag
    12601 cagccgtctc caggacaatg acccggatgc ggcttttctc gtccccgatg tcccggaaca
    12661 gcagcagcgt cagcggcaca aaggtgagca gtgccacatc attggtcacc agcatggcgc
    12721 tgaaaaagca cagctggacg agaaacagtc ccacacgccg cactgtcccg gcacgtttca
    12781 acaagttttg cgtcacctgt tcaaacacgc cgatgctgcg cagtcctgcc actgtgagca
    12841 tgagacaaaa cagctggatc agcacagcac ggttgcagta ggacaaatag ccggcatccg
    12901 gcggcacaaa gcacatggac accagcgccg ccagaagtga gatccccagc acaggctgtc
    12961 ctttccacca ttgcaaaaag cgtttcattg atacattctc ctttgttccc gtgattttcc
    13021 gcattgtttc cagtatagca cttttccgga aaatgtcaaa tgctgccgcc ggatgatttt
    13081 ccacatttcc ttgacttttc cacgattctc gtttatactg atatataata agaacttgtc
    13141 ttcacaagaa atatatgcgg attttcgagc ataattttgt tgcagacaag aaaaaaatac
    13201 gcaggcgggc tgtcgcccgg caagtatttt ttccgcagta tgcagcaaaa tttgccgaaa
    13261 agacacgtgt atatgcttat gaagacaagt tctaactcca ggaaaaaggt gattgtatga
    13321 aaaaggctct gattgccatg agcggcggcg tagacagttc cgttgcggcg cttctgatgc
    13381 agcaaaaagg ctatgaatgc gtcggtgcga ccatgaaact gttcgagaac gaacaggcag
    13441 gggtcaagcg ggaaaagacc tgctgctccc tggacgatgt ggaggacgca cggagcgtgg
    13501 cgtatcgtct gcatatgccc tactatgttt tcaactttac ggcaaacttc gaaaaagagg
    13561 tcatggaccg gtttgtccac gcctatgaaa acggctggac gcccaacccc tgcatcgact
    13621 gcaaccgcta tctgaaattt gaaaagctct atctccggat gcaggaaatg cagatggaca
    13681 ccattgtcac cggacactat gcccgggtgg catacgacga aacttccggg cgctatctgc
    13741 tgaaaaaggg gctggacggc agcaaggatc agagctatgt gctgtacaat ctgacccagg
    13801 aacagctggc gcacacccag ttcccggtgg gcgaactttc caagcaccgg gtgcgtgaga
    13861 tcgccgagga acacggcttt ctcaatgcgg acaagccgga cagccaggac atctgctttg
    13921 tgccggacgg ggactatgcc ggattcattg agcggtacac gggaaaagtt ccggagaccg
    13981 gcgactttct cgacccggag ggcaaacgga tcggcacaca ccgtggcgtg attcactaca
    14041 ccatcgcgaa agggtctggg catctctgcg ccccatccgc tgtacgtctg tgacatctgt
    14101 ccggcagaca acaccgtcac cctgggctac agcgagaacc tgttttcccg aaagcttacc
    14161 gcatcggatg tcaacctcat ctcctgcgac agcctggaag agccggttcg tgtccaggcg
    14221 aagatccgct accgccaccc ggcacagcct gccaccgcat ggatgacacc ggacggcacg
    14281 ctccacgtgg aattcgacga accccagcgt gccatcacct gcgggcaggc agtcgttctc
    14341 tatgacggcg atgtggtagt cggcggcggc agaatcaccg gcgctgaaaa ggaataaggg
    14401 ctgttttggt gcggtgctgc cgtgcaaatc aatttttaag aatgtatttt ggattcaata
    14461 ttgtcatttg agttataatc cccctgtccc ttcgggacat cccagcacaa ggcacaccct
    14521 acgggcgcct tttcaagggg gacatggtaa aaaaataagc ctcccttgaa aaggaaggtg
    14581 gcacgaagtg ccggagggat cattctcatg ccactaatat cccagttata ggtgcctttt
    14641 agaaaaatct tcttaaagac tgatttgcat aacgccgtaa caatttccac ttgaaaacct
    14701 ataaaaccta tggtataata ggataatcag catacgcaaa acaggaaatt tcctgtccgg
    14761 aaaggagctt atatgaaaat ctcgaccaaa gggcgttatg cgctccgaat gctgctggat
    14821 ctggcgcaga accagggtga cggctatgtt tccctcaagg acatcgccca gcggcaggag
    14881 gtctccaaaa aatatctgga gcagatcgtc ccgttactga acaaatccaa cctgctccgc
    14941 accaaccggg gctatcaggg cggttaccgg ctggcgaaag accccggcga ttataccgta
    15001 ggcgaaattc tccgcatcac cgagggcgac cttgcgccgg tggcgtgctt acagtccggc
    15061 gaggtggact gtccacgggc agagcagtgt ctcaccatct ccgtctggga agggctgtat
    15121 cacgtgataa accgctatct ggacagcatc accttacagg atatcttgga cagcggtccg
    15181 ggcagcggcg acgattactg catctgacca aaaaggcaat acaacaggaa cgaccctttc
    15241 aaaacgaata cgaaaaaatg cccacaccgg atgtattttc cgatgtgggc gtttgactat
    15301 tctaatcccc ctgtccctcc gggacatccc agcacaaggc acaccctacg ggcgcctttt
    15361 caagggggac aaaacgaacg acaggtggca cacagtgccg aagggattct catgctgata
    15421 catcagcata agacatagcg gtatggcttt ttcataaaaa tcagttctgt tccggcttga
    15481 tgacaacacg gtttttgttt ttcttgtcct ggtacaattt ctgatcggcg aacttcacgt
    15541 agttctggat ctgttcccca accttgcagg gcatggtata aacgcccacg ctcagcgtga
    15601 tgttataggg aatatcgctg gtctcgttgt acaccttggc agcctttttg atgctgtcgt
    15661 agatctgctg ccctgcctgt tcggcatcct ctaccacggc gaatccggca aactcatcgc
    15721 cgcccactct gccgatggca tctagcaacg gcacagcgga agtcagatac tgcacacagg
    15781 ttttcagtgc gtggtcgccg ccgtcatgtc cgaaggtgtc gttgatgatc ttcagattat
    15841 ccaggtcagc caggatcagc acacaggatt ttccggcaga ttccggactc tccagcagca
    15901 tattggacat ccggtagaat ccacggcggt tgtatacgcc ggtcagttcg tcggagtcgg
    15961 agatcagttt cagccggcgg ttgtcgaacc gtgcctcttc cagattgccc tccagctcct
    16021 tgaccagacg ggtcattttg atggcggagc agatctgggg cgctacggaa tagatctggg
    16081 agaagtggtt ataatccagc tcgcagataa acagaccgta ctgctcctca ttggagaaaa
    16141 gcggcaccat cacaaaggta taccgtctgt ctctgggcat atagcggttg gcgatgcagt
    16201 ggtatacgga gatctgctgt tccggcggct cgacacgctt gagcttctgt ccgatgtgat
    16261 aggacttcag atagagattc tccggcatga tccactcttc atactgatag tgtaccaccg
    16321 gctggttgaa cacgtagata taactgctct taaagttgac ccgatacaga ttcttgacga
    16381 tggagaaaaa gcttttttcc tcgtcgttgc tgtttatcat catatccttg ttgatattgc
    16441 tgataaggaa attgctgtag gtcagattgg tgcggagcag atactgctgc tgcatactgt
    16501 agtccagcag atgatccagg aacatttcgt ggagctgttc caccgcaagc cggttttctt
    16561 tgtcatgctg acagatctgt gtcgccaggt ctctggcata ggtcacgatc tttttcagca
    16621 gcggcacctg caccgggatc acatctgtgc ggtcgaacag ctgtgccagc tgctgacgga
    16681 accacggtat atccaagatc tgccggctgc atatcgtctg gaactcttcc cgtaccagcg
    16741 tccagacatc ttcaataaac ttggtctttg caacggtttc caggtcaccg atgtacttgt
    16801 tgaagatcat gttgacgatc tcttctgcgg cgcagttttc cgccatctcc tgaatctgtt
    16861 ccgtggtcag atcgcaggac tcccggacga tcagacgggc atccagggtc ttgctttccg
    16921 catgaccggt ctggagcagc cgcaccattt cccgcacgga ctgatagccc atactgctga
    16981 tgttggtgcg gacggtggtc agcatgggtt tcagactggt cgccacctca gaatcgtcaa
    17041 atccggtgac caggatatcc ttgcccacac gcattccacg ctcctggaac acgttatagc
    17101 cgccgatgca cattttatcg ttggcaaagc agattgcctc caggtcgggg tgcatatcca
    17161 gcagttcccg aaccacctct tccgaggatt cggagaagtt gccgtagacc accttggtgc
    17221 agtcgtaggg aatgtggttc gcctccagcg ccgtgcggta gcagtgcagc cggtccatgg
    17281 cgtcctggtt tcccatcggg ccggaaacat agccgatctt ccggcagccg tggcgctgga
    17341 tcagatgctc taccgccagc ttcatcccgt ccgtgttgaa ccggatgcag ggatagccgg
    17401 gaaattccac ttccatggtc aacagcggca gcggatcgta ggaatgcagg aacttgcgca
    17461 tttcgtcccg gctgatatac gagccgaccg ttcccacaga gaccagcagt ccgtccaagt
    17521 tggcggtgga aacatagttg tagatcacat tgtactgata atcatagatc gaatacttct
    17581 catcatcttc tacggcattg attcccctgc cgggcagcag gatcagattg atgtccagtt
    17641 cttccgcagc agcgatcgca cctttcagta tctcgcttgc atattcattt tccagatggt
    17701 gtatcatcag accgatctgg agccgttttt gtttcgtcat gattcttctc ctctgcgaac
    17761 attgccgccg tccctgtctg cccacagacc ggacggcatc catggaaaca ctgcaggcat
    17821 atcctgtgca aagcctttat gcggtgtctc cttagtaaaa aaacgctgcg ccgaccttcc
    17881 gcctgcatac gccggaatgc cggacacgtt tgatagcata tataagatac gatttgtgta
    17941 cttttgtagt atagcacatt cgacttgaaa aagcaagagt tttttcatat ttcatttcga
    18001 agaaaagaac tactttgttt tcttctgtac gcaattcaaa tgaatgtaaa aagttggacg
    18061 tgtgcattcc catttttgcg gaatgcgatg ctgtcttatg cctcgttttc cagggtgtag
    18121 cggcacgccg gataattgct gcacccgtag aaatcgccgt atgtgccgtg gcgcagcacc
    18181 agctttccgc cgcagcgtgg acacaagccg ttccgcaggg caaagttccg gtaggcgatc
    18241 tcgttgtcga tctgttccag gtgcgtctgt ctcgcctggg gcgtgtccag attggcgcag
    18301 agaatcgtcc cggcataata gcgcacatcg tcccatgcca gcagcatctg ccgctgacgg
    18361 cgaatggtgc gccggacttc ccggaaatgc accagctgtt ccacgctgtc ctcctcctgc
    18421 tccaccgcca gcaccgcatc atctgcaaac gccgcaatgg agaaaaagca gtcctcgtcc
    18481 agacccagca gctgcatcag cgcccggatg tgggcatagt tctgccggaa cgggctgcgg
    18541 aaattatacc gctttccgtg ccgctcctgc gtccagaact ccttgcgttc cgtcccggtg
    18601 atggtgccgt gatagttttt ggtctcgatg acgaatatgc catagaccga caccaccacg
    18661 tggtcgatct gggtgagccg cctgccctgc aatagcatca gatcggacag cacgatgtac
    18721 tgctttttgc tgagccagcg cagatgccgg gcaacctgct tttcgccctg tgcgcccttt
    18781 tgtccggaag tcagcggttt tctcatggcg gttctcctct tctgaaaacg aaacggcggc
    18841 agggatagct gccgcacggt ttctggttga ctgcaattgt ggtacgctgt tttagtttgc
    18901 ggaattcgca gcatctgccg cagccgctgt gtcatcgccg gacgacacac ccaccaggat
    18961 ggaagagtcc gaagatacca cgttggggta ttccccgttc cacttttcga tctgctggta
    19021 tttcagcacg ttctcattca gcgatgcgtt gatcttctcg ttggcgtctg cctctgcctc
    19081 ggctttctgc cggatggcgt ccgcctctgc cttggcggcg atgacctttt gctccgcatc
    19141 cgcctgtgcc tcaatgatct tctgttcctt ttcggtctcg gcacggagct tgttctgctc
    19201 tgccacctgt ttctgctcga tggcgtcgtt gaactccgca gagaagtcaa aattgatgat
    19261 gttgaactta tcaatcaaaa tgccgtattc ctgcaccttt tccgccagtg ttgcgccgat
    19321 ctcctcgccc acggcagagc gcttggtaat cagttcctcg gcggtatact gcgccgtgat
    19381 ggctttcatg gattcctgga tcgccggctg caaaacagtg tctgcgtagc cctcgccgat
    19441 ggtgcggtag atcacagcgg aatcgtcctt attgatgtga tagttgatgg caacgccgct
    19501 ggagacggtt tgcagatcct tggacacgcc gttggagtcg atctccgttt tctggatctt
    19561 gttgttcatc atgaccacct gctgtacaaa gggcatcttg aagtgcattc cctcatccag
    19621 cacacggtcg gagacctttc ccatagtgac gaccacgccg gtgtgtcctg ccgggacaac
    19681 ggtgactgca ccggcgccca cgatcagcac caggagaacg atcacgccca aaatgatacc
    19741 ggttcgcatt tttgcatttt tcataacagt ttcctttctg tgtctgccgt cagaacccgt
    19801 ttctgcggca atataaaaaa caagcagcaa agcggaatcc gctccgctgt gctgcctgta
    19861 ttatagcatg aaagggtaat ttttgtcaag aaatgcggtg ctgctgccat gcaggtcgat
    19921 ttttagggaa gtttgctttg aatcgtgggc atcatcataa gctgacagaa aataatccct
    19981 ccgtctgctc cttacggagc atccacctcc cttttcaagg gaggcttat
//
LOCUS       DAWENO010000020        18368 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1254_length_18368_cov_10.774259, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000020 DAWENO010000000
VERSION     DAWENO010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_18368_cov_10.774259"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5232..5241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7709..7718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10058..10067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3970 a         4901 c         5125 g         4332 t
ORIGIN      
        1 acctcccatc tgttcaaatg agcagtggga gcagaaacaa accgcctacc gtttcaggta
       61 tgccctcaga cctccagtat accacacttt gcaaaaatta tcaaacaaaa gcagcaccgg
      121 ctgtgggaaa tctccctgcc ggtgctgctt ttttatgtgt ttctattttg ttgtgcaatc
      181 atatctgcat ctgcatcagg actcttccag tccggtgttg atgccgctct tttccagtgc
      241 ggtcagatcg tcggagacgg tctccttgtc gccgcctttc tttttcggaa agaccagttt
      301 cagcaggatc ggggtaacga tggtggtgaa tacgaccatg ataaccagcg gtgtcatgaa
      361 cttttcgctc atcagtccca cagatgcacc cttgttggca acgatcagtg cgacctcacc
      421 acgggagatc ataccaatac cgatctgcag gctctccttg ttggtgtaac gcatgatctt
      481 tgcgcccaga ccgcagccga tcaccttggt gacaaccgcc agcagcatca gcagaagcat
      541 gaatactacc agctttttgg tcagaccctt cagcgtcatg gcgagaccga tgttggcgaa
      601 gaagatgggg gagaggagca tataagacag ggtgttgaaa cgatttacca cgtactcatt
      661 ctttttcagt ccggatactg ccagaccggc aacgaatgcg ccggtgatgt ctgctacgcc
      721 aaagcaaatc tcagccacga aggacagcag caggcagaaa acaaaggtca cgatgttgtg
      781 gcggcgcagg ttttcgttgc tggattcacg ccattttttg aagaagaagt gatacagcac
      841 agcagcgccg atggcaacga tgaagaatgc caggatgttg atgactacgg caaggacgcc
      901 gtcgcctgcc agcagagagc cgaaggtctt ttctgcctcg ccgtcgccgg acttctttcc
      961 gcccaggctg ataacgacgg tgagggcgat gatgcccagg atatcgtcaa tgacggctgc
     1021 acccaggatg gcgtttccgg cacgggaatt nnnnnnnnnn ctgccagaac agctttgcca
     1081 tctccggatc ctgttccgtg ttgtagaagt ctgccaggaa aaagccgcct accagcggaa
     1141 cgataacgcc cagcagagcg ataacgaagg aggcaaggcc tgcctttttc agttcctgga
     1201 tgtcggtctc cagaccggcg ctgaacatga ggacgataac gcccagttcc gccaggctga
     1261 cgatcaggtc atttcgctgt accacgttca gcaccatcgg gccgagaacg ataccggcag
     1321 ccagtgcgcc cacgacctgt ggcaggctga tccgcttggt cagcagtccc agcagtttcg
     1381 tggacagcag gataattgcg atttgcagta aaaaagaata atcattgttg aacaaaaaat
     1441 ctttcaaacc cataatggtt gatatctccc tttataattt gctttttgtc aagaaaacgc
     1501 tttgtcaaat tcgaactgac ggcaaagtca atttcccgtc agattctgcc gcagcaccgc
     1561 cgcatccagt ccgtttaccg tcttgtcggc gcagaggtct gcgttggcgg cgccggtctt
     1621 ggacaggctc ttgtttccca gcaggtactg gatcagcagc accaggtctg cggaattcac
     1681 tttgctgtcg gcgttgacgt ctcccggaag tgtctccggc ttttgcggct ctgtggtcac
     1741 atccggcgtt ttgccgtccg gttctctgcc ggagatcagc acaccgttct gatagacggg
     1801 aatgcagtcc gagatcacat attctcctgc atttttcagc agatccgtgt tagaatagtc
     1861 attgaaggca tcccagcccg tctgatagtt ggggtagatc agcgccagca tcagctcaca
     1921 ctggtgctgt ccctcagaga tgggcattgc cactctgccg tccggatagg tcacttccac
     1981 ataatagatg ttgtccttat aatgtttcag ttccgatacc tgcgccgggg tgtagtcgtc
     2041 gtacatcttc gcctggtcac ggtcgatgcg catgaccaca tcgccgggct ggaatccgcc
     2101 gtcaatgact tcggacagat ccatgtagta ccgataggac agattgtcct cgatccgtgc
     2161 cggccatgcg gagtggttgg tgagcttaaa gctcagggtg acgccgccgg aggtctccgt
     2221 cagcttggtc tccacataga attctctgtc ccgggtctcc ggttcgggaa agtccgggtc
     2281 gggcgtgccg ccgtaggcgt cggtcatctt gcagagcagc gccgtgaatc cggcgttgta
     2341 gtcgcaggca acctcgttgt tgatgtagtt ctgccggtcg tcctcatagg agccgtcctc
     2401 gttgggaccg cccaccagtg cgccgtatag gatgtgccgg ttggtctccg gggtgtccag
     2461 ggcgtttttc caggaggcgt gtgccgtccg gtggtgggga ttctgcggag acttgtcgcc
     2521 gtatcccacc acaaagctct gcccgtcggg attgtcgccc agacagtagt tgatctgatc
     2581 ctcatagaag ttctgatact ttgtggtatt cgtccctttc agcaccgtgt cgcacgccac
     2641 gctcgccagg aatccggcag tggtggcata gcgcagacag ccccaggtgg tgagccactt
     2701 tgcgccgccg tccagggcct tgacggaatc tgtccagtag tccaggtgtt tctgtatccg
     2761 tccgatgtag aaactgtcgt tggtgttgat ggcgtagagc agcagaccgc cctgcatcac
     2821 atcgtcccag cacattcccc aggagtattt cagctcatcc gaacccagct cttttcccag
     2881 attggggata taagaggtcg ccttgtccag ataggactgc tcgccggtgg caatgtacag
     2941 ccagtttgcc gcatagaaca gctcgtcata gaagtgggac gaacggtaga atccgctggc
     3001 atcgctgttg ttgtagtcgt cgtcgctccg ggtctcgtcc gccagccgga agagattctc
     3061 ggcgtgtttc agatagtcgc cggtcttgtc gctcctgccc ttgagggcgc acgccccggc
     3121 agccagcgcc gccgccatct cgccgcagac cgctgagctt gtgccacgga gagcagccct
     3181 cgccttggtg tagtcagcgc cgttgtcctt catgccgtat tccagcaact ccaccggacc
     3241 ccaccaggtg tgatccatct gcccgttgcc cacctggtat acggcggtgt cgccctcgtc
     3301 acaggcggcg aggtagtcca gcacaaagga gaggttgttc acataggtct ccatttcgcc
     3361 ggtcttttcc agaccgtccg ggtactgata cagtccccac gccagcatgg aggcggagta
     3421 tgccatgggc aggttgaact tcacgtggtc gccggcatca taccagccgc cggtgacttc
     3481 gtccgtcatg gtggagttgg cacgccattc cacctcgttc cagtccggaa gcggacctgc
     3541 ctgctggcac tcatagaagt agagggactt ctgcatcgcc tctgcatagt tggcggaaaa
     3601 atccgccgca gacacagtga gcgcagagca gggcagcgca cccagaacga gacatccggc
     3661 agtgagcgcc gccgtcagcc gcaagtgttt ccgcatttgc atcatcttcc tttcttgcag
     3721 ggcgcagcag agtcgtgctg tgtccccgca gattccggtc cttttccgcc ggaaataccc
     3781 ccagccgaaa aaagccgttt tcccttttat tatggcacgt tttcacgccg atgtcaagcg
     3841 gttttcatga agttatcaca aaaattcaca gtgaaacaaa ccggtttttt caaatttcgg
     3901 tgcatagccg tcatttccgg ggattctgtt gcagagcggc aaaaatgtcc gttgtccgga
     3961 tgcttccttt tgtagagaat gcttgtttca aacgggggat ttctgtacga agtgacaaaa
     4021 attacggaaa aacggattct gtcttgcaaa aaattccggt tcatgctata atgaaattgt
     4081 ataggcatat gcggtagtga tattccggaa aatggaatgg cgcattcgca gtctatggca
     4141 ttgcggcata caagggcagt tccgctccgg cagggcgttt tccgccggca gaacggatcg
     4201 ctttctttct gtgtgcgcat caaatataag gttttaaggt ttaggaggaa ttctgaagtg
     4261 gaaaagaagc tgaaaaaggg aaagaaagtt accgtcctgg gcgctggtaa tgtgggcgca
     4321 tccattgcat ttgctctggc aatcaagggc ctctgctctg agctgctgct catcgacatc
     4381 aacaagccca aggcaaaggg tgaagctatg gatatcatgc agggcaacgc attctgtccg
     4441 tcctgtgaca tccacgacgg cgattatccg gacgcagtcg attccgatat ggtcatcatc
     4501 accgttggta ttgccagaaa gccgggtcag acccgtatcg atctgtgcaa gaccaacgca
     4561 aagatcatgg catctgttat gccggagatc gtaaagtatg caccgcacgc ctgctacctg
     4621 gtagtcagca acccggttga cgtactgacc tatgaggcga tccgtgtatc cggtctggac
     4681 ccgaagcagg tcatcggttc cggtactgtt ctggactcct ctcgtctgcg cacctgcctg
     4741 gcagatcacg ctggtctgaa catcaagaac atccacgcat atgtattcgg cgaacacggc
     4801 gattcttcca tgatcccgtg gtctctgtcc agcattgccg gcatgaagtt tgatgaatac
     4861 tgcacaagcg tagaagatct gggcgacaag gatgccatcg agcaggaagt tcgtgacggc
     4921 ggcgctgagg tcatcaagtg caagggtgca acatattatg caatcgcact gtctgtcagc
     4981 cagatcactg aggcaatcct gcgtgacacc aaggcggttc tgacagtttc cactctccag
     5041 gacggcacac actacggcgt aaaggacgtt tgcctgagcc tgccgtgcgt actgggcgca
     5101 aatggcgtag aacgtgagat cacaccgccg atgaccgacg atgagatcgc tcgtttccag
     5161 aagagcggcg aggcactgcg ctccgtcatc gaccagatgg cactctaagc gcaagccatc
     5221 ccgtatctct gnnnnnnnnn nctgcactat tattatatcg tagcaagccg gcgcactgcc
     5281 gccgggcaaa cgactgccgt attttccgca ggttcccggt gagcctgcgg aaagagccgg
     5341 aaaggctctg acaaatgctt ccggcagcac tgtcggaaaa ccgaacgatt ggttggaaag
     5401 ggacgaaaaa attatggatt atgcagctga atctctgaaa ctgcactatg actggcaagg
     5461 caagatcgag gtcacacttc gtgcgccgct ggaaaccgcc gatgatcttt ctctggcgta
     5521 tacgcccggc gttgcacagc cctgcctgga aattcagaaa gatgtttcca agagctatga
     5581 cctgacccgc cggggcaacc tggtcgctgt tatcacagac ggcacggctg ttctgggtct
     5641 gggcgatatc ggcccggagg ctggtatgcc ggtcatggaa ggcaagtgcg cactgttcaa
     5701 gcgtttcggc aacgtggatg cgattccgct ctgcgtccgc tccaagaatg tggacgagat
     5761 cgtggagaca gttcgcctca tcgccggcag tttcggcggc gtgaacctgg aggacatctc
     5821 cgcaccacgc tgctttgaaa tcgaacgccg cctgaaggaa tgctgcgaca ttccgatttt
     5881 ccacgatgac cagcacggca cggcagtcgt tacgctggca gcgatgctca acgcattgaa
     5941 actggtgggc aaggatatcc acgatatccg tgtggttacc agcggcgcag gcgctgccgg
     6001 tctggcaatc atccgcctgc tgatcgctat gggtctggat ccgcaccatg tgatcctctg
     6061 cgaccgccac ggtgcgatct atgagggcag agacgatctg aacccccaga aggaagaaat
     6121 ggcgaagatc accaacagcg gtcacaaggc tggtacgctg gcagagatgc tggtggatgc
     6181 cgatgtattc atcggcgtat ccgcaccgca ctgcgtgaca ccggaaatgg tgaagtctat
     6241 ggcgaaggac ccgatcatct ttgccatggc aaaccccacg ccggagatca tgccggacga
     6301 ggcgctggcg gctggtgcag ccgttatggg taccggacgc agcgacttcc cgaaccagat
     6361 caacaacgtt ctggcattcc cgggcatctt ccgtggcgca ctggacgttc gtgcaagcga
     6421 catcaacgac gctatgaaga tcgctgctgc aaaggcaatc gctgacttcg taacgcccga
     6481 aaagctgtct gcggaatata tcattcccag cccgctggaa gagggtgtca gcgccgctgt
     6541 tgcaaaggca gttgcacagg ctgccaagga caccggcgta gccagagtgt aacatctgac
     6601 agcacaaaaa gaaaatcttg ggacagggga tattatggtc tcctgtcctt ttttgtggat
     6661 aatgtcaaat caaactgact gtacaaaaga atattaagtg aatttcccgt ttgacaattg
     6721 gaaatttggg aatttgtttc agatagaaaa aaacacaaaa accacttgca gatttgtgtg
     6781 aaatgtgcta aaatagacaa tggactttga atctgatttc gaagtcccat acagcgatga
     6841 cgcatctcgt ctcgcaaagc aggaaagatt gtctatgaaa cgaaaattac ccaagtggtc
     6901 gggcgtcgca gccctggttc tgcttgccgg tgcgctgggc gtactggcgt ggcagggcag
     6961 ccggcatctt accgcagcgt ccgggaacac accggctcgt ctggcagctg tctccgaaac
     7021 agttaccgac atcggcacgc tcccggcaga ggaatcccag ccctgtccgc agacggcggc
     7081 tgtgacccgg cagactgaaa cgtctgtgac atctgacagc tcgccgcaaa ccgcagtgac
     7141 gtttcctctg gaactgaacg ctgccggagc ggcggaactg gaacagctgc cggggatcgg
     7201 cgcagttctt gcgcagcgca tcgtggcata ccgtacacag atcggcggct tttccaaccg
     7261 ggaacagctg ctggaggtgg agggcatcgg tgcggcaacg ctggaaggac tgaacggctg
     7321 gattttcgtg gaaaacgaga cggcgctttc ggcagagccg gaacagccct tgccggagga
     7381 gaccccaccg ccgcagccgg cggaagaatc ggcgttgccg gagacttcgg cagagaccac
     7441 gccgctgatg ccgctgcccc tggatctcaa ccgggcgggg gaagaggatc tgatgcagct
     7501 gccggatatg cagccggcgc tggcagcaga aattgtggca ttccgggaac ggatcaccgc
     7561 attctcttcg gtgtatgaac tgctttatgt ggacggcatg acggaggcct atttcacgga
     7621 aattcgggat tctgtgcaaa tttacgggga cattgccccg gaataacgct tttttgagca
     7681 aactccatac tttaaccatt ttgtaaccnn nnnnnnnnaa ttgttctctg gcacagtttt
     7741 gtgcattttc acagaggaca gcatcggtgt gcagtgaaat gcaagtttgc tgcacgaaaa
     7801 aagtttattg cactgctatg ccaaaaccgg cacaaagtgt gatatatata tttaaggagg
     7861 aaaaattcca atgaacaagt tgaaaagaac actggctctg atggctgcat tggcaatgac
     7921 tgctactgca ttcgtaggat gcggcggcaa cgaaagctcc agctcttctg agtctaaggc
     7981 agaaagctct gctgaagaaa gcagcgaagc agatgcagat tccgctgaag attctaccgc
     8041 tgaagaagaa tcttctgttg agtccgcagc tggctacacc aacgttgatg ttccggctct
     8101 gggcgaaccg gacggctccc tgaaggacgg cggcgacaag ctgactatcc tgtgctggaa
     8161 cgatgatgac ctgaagtaca tgtacgacat cgttgctgat gcaaccggca tggacaagag
     8221 catgatcaac tacaagaacg ttggttctag cggtactgat gctaacgaac agtacgcaca
     8281 ggtattcctg tctggcgacg acgttgacct gtacttctgc gacgctgact ggaacatggc
     8341 ttatcagaac aacgatgagt actctgctcc gctgtctgca gtaggtatct ctgaagacat
     8401 gtatgcaaat gcttatggct acactgtaaa catgggcaag gacaagaacg gcgtcctgaa
     8461 gggtgcttct tggcaggctg cagcaggcgg ctacgcttac cgtgcagatc tggctgaaga
     8521 atatctgggc gtaacttctc cggaagatat gcaggcacag gtcggcagct gggatgactt
     8581 ctgggcaacc gcagctaccg tttatgagaa gtctggcaaa accactgcta tggctgactc
     8641 tctgggcggc gtatggagag cttactccaa cggtaacaga accaagtctt gggtaaacga
     8701 cggcaagctg gattcttctt ccgtaggtga cttcatctct atggctaagg agaacttcga
     8761 caagggttac atctctggtg caactcagtg gactgatgac tggctcccgc agggtcagtc
     8821 tgacggtgct caggctaaca agaccttcgg cttcttcttc ccgacttggg caatcgctaa
     8881 gggcggttct ctggaacagg ctgaaggcgg cgaaggcggc tctacatacg gcaagtatgc
     8941 actgtgcgaa ggcccgacat cctggttctg gggcggcaca tggatctgcg tatatccgaa
     9001 gactgataac gctaaggaag ctggtcagtt catctacact atgactgctg atgctgatgc
     9061 aatgcagaag tatgtagacg ctcgtggtga cttcgtaaac aacaagggcg taatgtctgc
     9121 tacaactgaa gctggcacaa acagcaaccc gctgttcggt ggtcaggatc agttccagat
     9181 cctgttcaac tctgctgaca agatcgacat gagcattgct tctgagtatg atgcaaagat
     9241 caacacagac ctgcagaacg cagttcagga ttactgcaac ggcgtaacct cttctgagga
     9301 tgactgcctg aacgcattcc tggatgcagt tgctgctgac gtaactgaca tcactgttga
     9361 ataagcttcg gctctcaaca cttttttgaa tgattgaacg tactgcctat aggataattg
     9421 cgtcctatag gcgtatgttt atcttttctc ttactattct actgaatttt tgaaagggtg
     9481 tgtgcaaatg gcttccgctg cacaacgtcg aatcaagtcg attagctatg cgaagtatgg
     9541 ctatatgttc attgccccgt tctttctcgt gtattgtttc ttccaggtat ggccgctgat
     9601 ccagaccttt atcctgagtt tccagggtaa cggcgctgat gccggtaact ttgtcggatt
     9661 cgacaactat ggcatcatcc tgttcggttc tggcgaaggc atgggccgcc gtgctgctgc
     9721 aatgcaggat ctgttcctga catcgttcaa gaacaccatc atcctgtggt ttggtaactt
     9781 cattccgcag atcctgttgt ccctgctgct ggcagtttgg ttcaccgatt cgaaactgca
     9841 cattccgggc gtgggcttct tcaaagttgt tatgtacctg ccgaacatca ttactgccgt
     9901 ttccgttgca gctctgttca tgcgctttat ctccaacagc cagctgtccg cagtcaacac
     9961 actgctgatg ctgaatggcg gcgatccggt tagcttcgaa gcatctgctg catggagccg
    10021 tggtatcgta atgttcatcc agacatggct gtggtttnnn nnnnnnntgg tttggtaata
    10081 ccatgatcat gatgatgtct ggtatcatgg gtatcaaccc gtctctgttt gaggctgccg
    10141 caatcgacgg cgcatccagc ggacaggtgc tgcgcaaggt aactctgccg ctgctgcgtc
    10201 cgatggttgt ttacacactg atcacttcca tgatcggtgg tctccagatg ttcgatatcc
    10261 cgtatctgta tcacacaggt gctggtaaga tcaacgcaaa tctgcgtacc gttgcggtat
    10321 tcgtttatga acagttccgt gccggtgcaa aggttcacca gcctgactac ggcattgctg
    10381 gtgctgcttc cgtaattctg tttatcatca ctgcggctct gggtattctc gtattccgta
    10441 tgaaccgtga tgaggatgag catcgcaaaa agcaggagcg caaggctctt gtcaaggaat
    10501 ataagagaca gcagaaactg gcgaagaatg gaggtctcgg cgcataatgg aaaacgttgg
    10561 tgttaaagac aattatatgt tgattcagag actgaaatct gccggatgct tcattgtctg
    10621 cgtcctgctg gcattgatct gtctggttcc gctgtacatc atgatcatga acgcaacgca
    10681 cggcgacgct gctctgcgtg cgcagccgct gcagttcctg ccgggcagtg acttggtgaa
    10741 gaacatcaag aatattgccg gcggtgaatt tccgcagtcc ccgaacttca acgtttggct
    10801 gggctaccgg aattctctga tcattgcagg cggttctacc atcctgacag tattcttctc
    10861 tgctttgact tcttatggtc tgactgttta cgatttcaag gttcgtgacg gcgcatatac
    10921 attcatcctg gcagttatga tgatcccgat ccaggttaca tccaccggtt tcgtaaagtt
    10981 catggttggt actctgggtc tggcaaacag ctacatcccg ctgattatcc cggcgattgc
    11041 agctcctgct gttgttttct acatgagaca atatatgaag tcttccttcc cgctggacat
    11101 tgttgaggct gcccgtatcg acggaagcgg cgagttccgc accttcctca ccattgcgat
    11161 cccgatgtgc aaaccggcaa tcgcagtaca ggcgatcttc gcattcgtac agaactggaa
    11221 caacttctat acacagaata tgatcattat ttccaacgaa aagcgtttca cgatgccgat
    11281 catgatccag agtgttctgg gtcaggataa gcatccggat ctgggcgcac agtacgctgc
    11341 agttgcactg tctgttatcc cgatcatcgt gatctacctg attctgtcca gattcatcgt
    11401 tgctggtgta gctctgggcg gcgtaaagga ataatcgcat ttcaacagaa tgtttgttct
    11461 tttcaaaaga gtcattgcct tcgggcagtg gctctttttc tatttcccgt ggatgtttcc
    11521 gggcaaaaga acagccctcc ggtcgctgca ccagagggct gtttggtgtt ttcatttcag
    11581 tgtgtgctta tttctgtttc agttccgcag cacggagggt gtttttcatc agcatggtga
    11641 tggtcatagg accaacgccg ccgggaaccg gtgtgataaa gcttgccttc ggctcgacct
    11701 cggcgaattc cacgtcgccg cagagcttgc cgttttcgtc gtggttcatg cccacgtcaa
    11761 tgacaactgc gccctctttc accatgtcgg cggtgatgaa gtgcgctctg ccaacggcgc
    11821 tcaccagaac gtccgcagct gcgcagactt ccttgaggtt cttggtgtgg gagtggcaga
    11881 tggcgacggt tgcgttccgg tggaggagca gcatcgccat gggctttccc acgatattgc
    11941 ttctgcccac gaccacgcag ttcttgccgt ccaggtcagt gccggtgctg tcgatgagtt
    12001 ccatcacacc tgccggagtg caggggagaa agtcgtagtc gccgatcata attttgccca
    12061 cgttgatggg gtggaatgcg tccacatcct tcagcggcga aatgctgtag atgaccttct
    12121 gctcgtcaat gtgttccggc agcggcagct gcaccagaat gccgtgtacc ttgttgtcct
    12181 tgttcagaac gccgatcaga tcgaggagct gttcctcggt ggaattttca ttgagccggt
    12241 attcaaatgc gtgaaagccc acttcctcgc aggctctctg cttgttgcgc acgtagatct
    12301 gggatgccgg gtcgtttccc accagaatga ccgccagtcc cggctggatg ccgctgtttt
    12361 cccgcagcgc ctgcgcctct tccttgattt tttggcgaat ctgggcggaa acttccttgc
    12421 ccgaaatcaa ctttgccata ttgcgttact ccttttcgtt ctcattctca caattttctg
    12481 ttgcagtatc atcattgtcc gcagatgcgt ccgatgcgtc agcctctgtg gtagtatctg
    12541 tagtatcgtc tgtgctttcc gtctcgttgg attcgctgtc ctctgcggtg tctgcgccgg
    12601 tcttgccagc ctcatattcc gccagcatcc gcttggattc cgccgtgtcc ttgtacatat
    12661 gatagtccac gcccatgcgc ttgacctttg taccgatggc aatttgcagg ataacggaaa
    12721 ccactacgca gcagattgcc agaatctggg acacacgcac gttgccgatc accaggctgt
    12781 ctgtgcggag accttccacc acggcacgtt ctgcgccgta ccagcagata tagatgagga
    12841 agatctgtcc gtcatagcgg cgaattttct tggcgaaaag cgccagcagt acaaagccca
    12901 gcaggcacca gacggattcg tacaggaaac aggggtgaac cggctgagac gcatccagcg
    12961 ccgtgccgaa gttggcgaaa taggtggtgg agggatactg gtcggtgatc cactcctgga
    13021 ttctgccgcc ggacattccg aagatgctgt tggtgttgca gccgaacgcc tcctggttga
    13081 agaagttgcc ccagcgtccg atgccctgtc ccagcaggaa tccgatgccg acaatatcca
    13141 gcaggggcag gaacttcacc ttgcgtatct ttgccaccaa aagtcctgcc agaaagctgc
    13201 cgatgatgcc gccgtaaatg gcgagtccgc cttcccggat attgaggatg cttttccagt
    13261 cgccggcgta gttgttccag ttcagcagaa cgtaataggc acgtgcgccc accacgccgc
    13321 cgatgatgcc gccgatgatg cagtccatga cacggtcggg gttcagcccg tactttttca
    13381 cctgggtgaa agcgtagacc atcgccagca ccagccccag ggtgatgagg atgccgtacc
    13441 actggatgtc tagaccgccg atggaaaagg cggtgctgtc caggtgcagg tcgatcccca
    13501 gtttcggaaa ggtaatttcg ttatgatcca gtcttccgat tgtttctgtc gtcatatgtt
    13561 tcccgggatc tcaaaggaga tccctgcctc ctttgggtta ttttgccggc gcaatgatgg
    13621 agagcgtcat gcgctccaca tccacttcct cccggaggaa acagttgcag tcctccaggg
    13681 tcatctcaga cagcacctgc atggtgtcgt agagaccgca gccagccatg tgggatgcca
    13741 ccagcgcatt ggtcactgcg gagacgttgt tctgctcccg caccagattg ccgtaggtgg
    13801 tctttttgca ctcggtgaac atggtctcat ccagcccgtt tttctgtgca tccagaatgg
    13861 cagcggtgat cttttcccgg actgccttgg ggtcacggga ttcgccgccg aagatcatgg
    13921 tgaagaaacc gtcgccgttg aacacttcgc tggaaaagct ttcgttcacc agtccgctct
    13981 ggagcatttc ctggtacagg agagacgagc tgccggacag ggtcagcata gcgatgtgcg
    14041 cctgcatctc tgcacgcaga ttgtccatgc cgctccgggg ctggcacttg atgcccagat
    14101 tgaacagcgg ctgtcccact gcggcagtct ccaccgtctc cggacgaacg atgtccaggg
    14161 tctcttccgg gaaaacggtc tccagtttct ggtcgtcgca gggtttcagc agacggtctg
    14221 ccacttccag tacctcatcc acagtgcagt ttccggcaac cgccagcacc atgttgtgga
    14281 gattgtagaa ggtgcggtag cagcggtaca gcagatcgtc tgtgatctgg gcaatgctct
    14341 cgatggtgcc ggcgatgtcg atgcgtaccg ggtgtttctg gtacagaccg cacagcatat
    14401 tgaagaacac acgccagccc ggatcgtcgt cgcacatccg gatctcctgc ccgatgatgc
    14461 cctgttcctt ggcgacgctt tccggtgtga agtacgggga ctggacaaag gaaagcagga
    14521 tctccaggga tgccttgaag ttgtcggagc agctgaagaa atagcaggtc ttgtcgaagg
    14581 aggtgaacgc attggcgttt gcgccggtct tggcgtacag gtcaaagaca tcgcagtcct
    14641 cgttctcgaa cagcttgtgt tccaggaagt gggcgatgcc ctccggaacg gtgcagtagt
    14701 ctttgtcggc agcggtcttg aactgggtgt tgattgagcc gtacttcgtg ccgaacattg
    14761 cctctgtggt gctgaaccct tccatctcgc agaccaggat ctccagaccg ctcttgtgat
    14821 ggatgtgggt gtatttgtca ccggtgttcg gggaactgat ggtggtcatt gtggtctctg
    14881 ccatgttact ccgcctcctg ttctttgcat tccataatat aaatggtatc cagctgcatg
    14941 gactttgccg ccgccatcag atcttccttg gtgaccttgc ggtagttctc ttcctcttcc
    15001 gccggcgtca gctttgtgcc acggcagaac tgtccgaagt accaggagat atagctggtc
    15061 gccgtgtcac cgatgccgca gagggtgttg tgaatggtca tcatggtgct gtccatctct
    15121 gcatccgtga tgtcgccctt gcagatggca tccagctgtg cggcgatggc atcctgtgcg
    15181 gtgtccaggt tctcggtctc aatgccgctg tccacctgca tgaagtgctt tgcctcgttg
    15241 tagtttgcgg cgcagtaata gcacagactc atcttttccc ggacgttggc gaacagcttg
    15301 gagctgggtg tgccgcccag gagcattgcc atcatcttca gcgcatagcg gtcgggatag
    15361 gtggttttcc agcccatgac cattttgcac tggttcaccg gaagtgcctc ccgtaccaca
    15421 gccggttctg ccttgcaggg gctggggttg aggaactgca ccggtgtcac gctgcggttt
    15481 tcgatggcgg cgaaagcctg tttcagggtg tcagccagct gcggcacagc tgccggaccc
    15541 acaaagtaga tttcgatctg tgcagtgcgg agcagttcct ggaatgccgc ataagcggct
    15601 gccggtgtca gagcctctgc ggcgctgcgg ctgccgtagc aggagaacgc tgccggttcg
    15661 ccggcgaagg cagtcttata ggtctgagaa atggcatact gccgcttttc gttgatctct
    15721 gcgtcaatgc tgtccagcag gttcatctgc tggatgcgga actcggtgtc gtcgaaagcg
    15781 ccgtccgtca cattgggctg gaaaatgcag cccagcacga tctccagcag ctctgcgaaa
    15841 acagattcgc cgtccagcgc atagcggtca gcgatggcgg aagagcgcag ggtgatggac
    15901 tggacatcgc cctgtttggc aatggtgcag ctcagatcag ccccgtagag cagatgcagc
    15961 ttgcggttca ttgccgcatt ggagggatag gctttgctgc tggaaaccac aaggctcccg
    16021 gcgagggcgc aggcagcagc agtcttttcc gacagaggct gcaaaaactg gatgcgcagg
    16081 tgattggtct ttagtttgga gtccaggatc tcagaaaacg caatgttgtc cgagagcggg
    16141 atccgctgat aactggttgt cataacatcg tcctttccaa aggattgcgg cgctgtaaca
    16201 gcgtctgaaa agttgttttc catacatagt acacatctgc gtgtcaatat ggattctggc
    16261 gtatccctgc ttttgcaggc atacaaattt attataccac atttcgctgc ggattgctac
    16321 ggttttcacg aaattctcag aaatattttc cgcagagaat ttccgggcgc tttttgcgat
    16381 ggtttacaat ttgttgttga aaagaaaacg tttttttagt ataataaaat atgaaaacct
    16441 atcgaaggat tcctgcaccg gaaaaagggt gcagaaagat atcatctcca acaggaggaa
    16501 aagcaagatg aaaacaatta cagtacacgc cagccagacc tatcccatct ggatcggcag
    16561 gggactgctg ggacagtgcg gcagctatct gaaacagcag accaaggcac agacggcggc
    16621 ggtcatcacc gacgaccatg tggacaaata ttacggcgac atcgtgacga gttccctgga
    16681 ggcgagcgga ttccgcacgc tgaagtttgt ctttccccac ggcgaggcat ccaagtgcag
    16741 caggacgctg aaccagatct atgactttct ctgcgagaac aacatcaccc ggacggactg
    16801 cctggtagcc ctgggcggcg gcgtggtggg agacatcacc ggttttgcgg cggcgaccta
    16861 tctccgtgga ctggactatc tccagatacc caccagcctg ctggcgcagg tggattcttc
    16921 tgtgggcgga aagacagcca tcgacctgcc cggcggcaag aacctggtgg gcgcattcaa
    16981 gcagccggtg gcggtgctgt gtgacctgga taccctggac actctgccga aaatgttcct
    17041 ggcagacggc atgggcgagg tcatcaagta cggcatgatc cgggacgaaa agctgtttga
    17101 actgctggaa cagcacaacc tggaaaatgt cagcagcgcc atggacgaga tcgttcccac
    17161 ctgcatcgac atcaagcgtg acgtggtgga gcaggacgag ttcgacaccg gcgagcggat
    17221 gattctgaac ttcgggcata ccctgggaca cgccgtggaa agctattatc actacgagac
    17281 ctatacccac ggcagcgcag tggcagccgg aatgtgcatg atgaccgaaa agacctgcac
    17341 tccggaactg tacgtgcggc tgtgtgactg cgtgaaggca tacgacctgc ctaccgaagt
    17401 gccggcaccg ctttcggaat tggttccgct ctgcggcaac gacaaaaagc gtgccagcgc
    17461 agccctgcgg ttcatcgtct gcgagaccat cggcaaggca gagatccgca gtatgccctt
    17521 tgacgagttt gccgtttgga tgggaggaga cgcatgaaca tactgatctc accggagaag
    17581 ctccacggca ctgtgcgcat cccggcatcc aaaagcatgg cgcaccgtct cctgatctgt
    17641 gcagctctgg cagagggaac ttccgtggtt tccggggtgg atatgtcacg ggacatcacc
    17701 gccaccatgg acgtgctttc cgcattcggc gcatcgttcc ggcaggacgg cagcaccatc
    17761 acagtcaccg gcgtagggaa gaccagaccc caggcggctg tggcggactg ctgtgaaagc
    17821 ggctcgaccc tgcggtttct cattccggtg gcggcggcac tgggtgtcca caccaccttt
    17881 ttcggacagg gcagactgcc ccagcgtccc atcaccgcct atctccggga actgaccaaa
    17941 aagggcattg cgtttgagta tcacaacacc atgccgtttt ccattgacgg cgcactgcaa
    18001 tccggtgtct atgagctgga gggcgatgtg tcctcccagt atgtgaccgg actgcttttt
    18061 gcgctgccgc tgctggaggg cgattcggag atacggatgc tgtcccggct ggagtccaag
    18121 ccgtacgttg atcttaccat cgcctgcctg cgtcacttcg gcgtgactgt ccacgagacg
    18181 gaacagggct acaccgttcc cggcggtcag cagtaccatg cctgtgacat ggaagtggag
    18241 ggcgattatt cccaggcggc gttcttctat gtggcgaacg ccctgggcag cgacatcacc
    18301 ctcaccaatc tgaatccgga aagtgtgcgg ggggcggggg gtagtgggga gctgaggcgg
    18361 ccgggggg
//
LOCUS       DAWENO010000021        18330 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1255_length_18330_cov_9.583529, whole genome shotgun sequence.
ACCESSION   DAWENO010000021 DAWENO010000000
VERSION     DAWENO010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18330)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1255_length_18330_cov_9.583529"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8407..8416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4440 a         4677 c         5189 g         4014 t
ORIGIN      
        1 tggaaacaaa agaaaattca aaccgcctac cgtttcaggt atgccctcaa ttaaccatta
       61 taccagacgc atcgaaattt gtcaaatcaa aaaaagccct tgacaaacgg ggcatgatag
      121 cgtataatag aaatagaaag agcctaccag tagacggttt gctcccattc gtgattattt
      181 gaaataaccg cctttagttg gaagctgtgg ggcggttatt tccgtttctt gaagtttaag
      241 taagcaagat atgctttgaa tacttcacaa accatgctaa tgcacttgac gagaatgtct
      301 atgttcattt gcactcactc ccttccggga gctggaatcg accgcctacc gtttcaggta
      361 tgccctcgat taaccattat accagacgca tcaaaatttg tcaaatcaaa acaccgcatc
      421 gcttttcgga aacatccgaa gaacggtgcg gtgtttttgt gtgcagtgct gttcttattc
      481 cacctgcatg atcgctcctg tgatctgccg gagaatgggc gatgcggctt gatttccata
      541 gccgccgtcc tctgccagaa cagtcaccgc atatttcggt tgttccgccg ggaaaaatgc
      601 cgttacccag ccgtgacagt actcggtccc gtcaacgtca cagcgcccgg tctgggcggt
      661 ggaggttttc gcacccatag agacgtgcgc cggtctgccc tggaagtcct cgtcggcggc
      721 agtgtaacac atcagccggc ggagaaatgc cgctgtttcc ggtgcaattc ccgattccgg
      781 gatgccacgc tcttcccgca gcaccgtcct gccgtcatcc gtgacaccac gcaccagccg
      841 cactgccggg agcgtgccgt ctctggcgat ggcacaggtc attctggcga tctgcagggg
      901 cgatgccgtc agaacgccct gcccgaagga aaagttcgcc tgttccgccg gaagtttcag
      961 ctgctgtaac gtaggcagcg tgccgccgga tgcgatcatg tttgaggtca gcatgatctc
     1021 cgtgccgaat cccagggact ttgccgtttg cagcaggtct gacccggaaa gcgcctgtcc
     1081 caaggcaatg aaataggggt tgcaggaact gcgcatggca tctgccatat gctgcacgcc
     1141 gtgtccctgc aattcgtgac agcgaaagcg ctgcgttccc acagagatcg ccccgtcaca
     1201 gtcccaggtg aaattgtagc ccacgccctg gtcaaaggca cacgccgccg tcaccagctt
     1261 gaagatcgac ccaaccggat acgcatacaa ggcacgctgt atcatagggc tgtcggggtc
     1321 gctgacggca tcgcccaggg agtcggcaga ataaccggga aagctggcaa gccccagaat
     1381 gtcgccgctt tccacgtcca tgaccacgat acagcccttt tccagtccgc tgccggcgga
     1441 ttcgcagatg cgctggatgc ggctgtccag ggtggtcata atgcctgcgg tgggatttgc
     1501 gccgtagcgg atctgtttcg gctctccggc aaggggcgtg cctgtgccgt ctaccgaaaa
     1561 ggtcacgctc cactgggaat ccacactgtg caggatggca tcataggcgc tttccagtcc
     1621 tgctacgccg ctgccctccc gggtatagcc gatcacgtgc tgggcaagct gtggcgacgt
     1681 gctgcgctgg gggatctgca gtatggtcac atccgggcag tcgatgtccg ccgtgtctac
     1741 cgtgcaggtg aacggcattc cctgtgccag cttttcataa aacgctgcgg tgtcggtgac
     1801 gtgggacagc agtgcctcgg ctgccgcagg cgtagggctg acgactgcga tgcaggcggt
     1861 tttggtattc accagcggca cgccgttccg gtcgtagatc gtcccccggg cagtcccggc
     1921 ggtgcaggta tacgtgccct gtctggcggc ggtctgtatc agctccggct tctgcaccag
     1981 ccatgccaga tatactgccg ctgtgcagaa cagcagcacc aacagcagac gcaggaatgc
     2041 cagacgattt ttcagctgct gcatgatctc gctcctttct ctgtgcggcg cttccgaacg
     2101 tgccgcctgt cacagatatc ctttgttttc tgcggaattt tatacaattt caactcgaca
     2161 ctttcctgca tcttgtgtaa cacctacaaa ttacgacagt tttttgtgga aatgttttct
     2221 cattttttgc gaaatccctt gacgaactgc ccgaaaaaga gtataataga ggagtaggaa
     2281 aaaactgtgc atgggcggaa ctgccgccgc acagacactg caaatagaaa gcgccgcaaa
     2341 aaggtatgct ttctgcgata cataggaaag gagtatgtgc cgcttttcgg cacagtagat
     2401 tggatcatga aaaatatttt gtttgctgcc tctgaatgcg tccctttcat taagaccggc
     2461 ggtctggcag acgtagtcgg cgcactgccc aagagtctgg accgcagcga gtacgatgtc
     2521 cgtgttatca ttccgaatta tacctgcatc ccgtgggaat accggagtca gttccgctat
     2581 gtcagccact tctacatgga ctacggcaga cacgtggaga acgtacacgt aggcattatg
     2641 gaatacgtct atgagggcgt gcatttctac tttatcgaca acgaatatta ctttaagtgt
     2701 gaccgtccct acagtgactc cacccagtgg gacatcgaac gctttatctt cttctccaag
     2761 gctgtcctgg cggcaatgcc ggtgatcgga ttccatccgg acatcattca ctgccacgac
     2821 tggcagaccg gcatgatccc cgtattcctc aaaagcacct ttgcgaccca cagcttttat
     2881 tggggaacca agagcatcat gaccatccac aatctgaaat tccagggcgt gtgggacatc
     2941 cgtcactttg tctataacac caacctgccg gattatatgt tcacgccgga taagctggaa
     3001 ttcaagagcg atgccaatat gctgaaaggc ggtctggtct atgcagacta tatcaccacc
     3061 gtcagcgaga cctatgccga agagatcaag acaccgtatt acggcgaaca gctggacggt
     3121 ctgctctgcg cacgttcccg ttcgctccgt ggcattctga acggcatcga ctatgaggta
     3181 tacaatccgc agaccgataa gaagatcgac gtacactatt ccgtcaagga cgctgtggag
     3241 aagaaaaagc aggcgaagct ggcgctccag aaagaactgg gactgccgga ggacgagaac
     3301 aagttcatga tcgccattat ctcccgtctg accgaccaga agggtctgga tctggtggac
     3361 tggatcgccg accgcattac agacgaattc acccagttcg tcatcattgg tacaggcgag
     3421 gctcgctatg agaatatgtt ccgctattat cagaacaaat atccggatcg tgtttcggca
     3481 aatattttct attctgatcc gctggcgcac aagctgtatg ctgcggcaga tgctatgctg
     3541 gtaccgtccc ggtttgagcc gtgcggtctg acccagctga tttccctgcg ctacggcacg
     3601 gtgccgatcg ttcgggagac cggcggtctg aaggacactg tcgtacctta caacgaatat
     3661 gaaaagaccg gcacaggctt tacctttgcc cactacaacg cagacgatct gctgtacacc
     3721 atcaactatg cgaagaagat ctacttcgac caccgtgagg actgggatga tatcgtccgc
     3781 cgtggcatgg agcaggattt ctcctggagc agctccgctt tgcagtacca gggaatgtac
     3841 gactggatcc tccgtggcat ggtagactga gcggagacaa aaaacgtcta ttgacacatc
     3901 ccggaaaatc atgtataatg agagcgggaa caagggaaac actgcacgca gattgcgtgc
     3961 aaagccgtca ggcgggcttt attcagtgtt ttccaagatc ccggaacaaa cgccgctatg
     4021 gcagattgga gttttaccca tggcagagga aaatatgctg gatgagagta tgcagggaat
     4081 gctgtatacc ctggaagacg aagagggaaa cgaacaggag tttgaggtcc tgggcgagat
     4141 ggaatacgag gacgcaaact actgcgcact gatcccgtac tatgagaacg aagaagatct
     4201 gctgaaagat gacggcgaat tcgttgtact gaaacgtgag atggtagacg gtgaggaaat
     4261 gctctgcacc atcgaggacg atgacgaata cgatgcagtc ggcaatctgt tcatccagta
     4321 tctgagcgat ctgtatgagg atgaggaaga agagcaggac gcacagtaac tgccgttttc
     4381 cacaaaccga ccagaaaaat tttcacaaaa ttcctattga tttttgtgaa agatcgtgtt
     4441 ataataaaca tagaaactga aacatcagtt ctgaaaaagt gaaaactact gcccagttcg
     4501 agacattgcg gtttttataa tccaacactc cttgaaaggg aatctgactc cgtctgtgga
     4561 ttgcagacct cctgtttacg gcaatgaccg taacggacga agggagcgtg aagcggaagg
     4621 gaagataccc accttgctat tatagcttgg ttttattcgc tgcaagacgg ctgggtggtt
     4681 tttcttttaa ccacacaaag cactctgtga gagactcacg gggtgctttt tcttttctgt
     4741 tgtgggagat attcgaaaag agttcacctt gaacaggggg atatgttcta aaatgatatc
     4801 ttacccgatt gatgtcggac atatccaccg acagaggaga cttttcggca gggaaaaatt
     4861 gacctgcgca gccattcaaa aaaacaggtt gtaattttcc ggaaaatatg ctacaataaa
     4921 aatagtgttg cccaaaagca atctcaatca caacaaggag aaaagacaat atgctgttac
     4981 gcatgacaga tatccataaa ttttataatg gcagacaggt tctctcccac gtctctctga
     5041 cggtagaaga ccacgaccgg atcgggcttg tgggtgtgaa cggctgcgga aaatctaccc
     5101 tgctgcgcat tctgacggga caggaactgc ccgaccatct gctggagggc gacggcgaaa
     5161 ttgccaagtc cgccaagacc agtatcggct acctggaaca gatgggcggt ctggatcggg
     5221 aaagcacggt ctgggcggag atgcgcagcg tgttccgtcc gctgctggac acccaggaac
     5281 gcctgcacca gctggaagag cagatgcagt ccggggatat gtctgcggca gaggagtacc
     5341 accgcctgca aacctggttc gaggacaacg acggctatca gatcgacgtg aaaatacgca
     5401 ccgtcctcaa cggtatggga ttcggacagg agacctatga ccgtgtgatc tccggattca
     5461 gcggcggcga aaagacacgg cttgccattg cacggctgct gctggaagcg ccgaacctgc
     5521 tgattttgga cgagccgacc aaccacctgg acttcaaaac cgtgatgtgg ctggaagatt
     5581 acctcaagga ctacaagggc gcactgctgc tggtgtccca tgaccgctac ttcctggatc
     5641 gtctctgcac cagcatctgc gagatcgagc gtggcacgct gacacggtac aagggcaact
     5701 acaccacctt tacccagctg aaagaggcgg ctgtggcacg ccagtggaaa gaatacgaga
     5761 tgcagcaaaa ggagatcgcc aagctggagg actacgtggc acgtaacctc acccgggcat
     5821 ctacggcgaa gagcgcacag agccgggtga aacagctgga aaagatggag cgcatcgaaa
     5881 agccggtctc tgcccagaaa caggcacggg tgcagttcac ctatgagatc gagccgccgc
     5941 aggaagtgct gaaagtgcag aatatcgaca tttccgtggg cgagggtgca gagcgaaaga
     6001 ccctgctgcc ggagttgtcc tttgaggtgc gtcgtggcga aaaatggggc attatcgggg
     6061 ataacggcat cgggaaatcc accctgctga agattttgca aaacaagctg ccccaccagg
     6121 gaaaagtccg ctggaccagc aacatcaaga tctcctattt cgagcaggaa agcaccaacc
     6181 tgcatccggc aaagtcggta atgcaggaga ttcacgaccg tgtaccgtcc tggacagacc
     6241 tggaagtgcg caagcttttg gggcaggtgc gtctgaccgg ggaaaacgtg ttcaaaccgg
     6301 tgggcgttct ttccggcggc gagcgtgcaa aggtctgctt tgcggtgatg atgctggaac
     6361 acggcaacgt gctgattctg gacgaaccca ccaaccacct ggatattgcc atgaaagagg
     6421 tcatcgaaga ggcaatgcag tcctttacgg ggacgctgct gtttgtctcc catgaccggt
     6481 atctgttgga taaggtggca gaccacatcc tggaactgaa agtggacggc gctgtcacct
     6541 accagggcgg attccagaag tggctggagg cgcagcagaa acagcccgaa ccggaggaga
     6601 cgccggcggc aaagacagcc gcatccggcg gaaagcctgc ggaaaagccg gcgtaccgtt
     6661 ccaagaaaca gcgtgcgcag caggcgaaaa tgcgtgccga cctgcgtagc ctggaacagc
     6721 agctggacaa gatgcaggaa gagctggaca cccttaccgc agagctgacg gacgaggcgg
     6781 tgtgcagtga ctaccagctg atgcaggaga agtgccagcg catggaggaa ctgcgtgtgc
     6841 agatggatga gactatggag cagatgatcc tgctggaaga ggaattggga taacagaaga
     6901 agatatgaca taacaaaaac cggagagagc atttcgccct ctccggtttt ctattttccg
     6961 cttgtatttt cgaaaagctg cttacagctt tttcttttcg tcctcgatca ttgccagcag
     7021 ttcatccaca gatgcgcttg cgatcttgtc ctcacgggtt ttcggtactg ccggagctgc
     7081 cggtttctgt tccggtgctg ctgcacggct gctgcggaat ggtgtgtcac ccaggttccg
     7141 tgcctcttcc ggagagcttg cctcgtggct gccacggtaa gtgggaaccg gagctgcggt
     7201 ctcatccggg atgttgttct cgatcggcgg tgtccggaag gtatcggtgc tgttcagatt
     7261 gctgcgtgtg ccaaatgtag atgtgttgga aacgctgcct gcgtgtgcgc cctcataggt
     7321 gccggtgttt ccggtgggct ggctgttctg tcctgcgccg cccagcggga tctgatctgc
     7381 cggattggtg tagatcggta cgtcatgctg tgccttgaac tgcatggtgc gtgccttctg
     7441 atacggagag ttggggttga cctgcttgcg ctcaaagttt tcagcgatag aagaacgctt
     7501 ctgctcaaat gaacggctct gcgcagtgtt ctcagacgga cggaacagag gagaacgtcc
     7561 gcccagttcc tcatcatcat aagcggaatc gtcgtcatcg taggaatctt cgctgtaaac
     7621 ttcatcgtag ttttcttcaa aggtatactc gtcgtcgtgc ttgccgttgg cacgtgcaat
     7681 ggcaacgatc agcatgatga ccaggatcac gctggggagt accagcaggg tcaggatacc
     7741 cggaacgccg gtagcaaatg tcacgtagct gtacagctcg ccgctttcat aagcgcactt
     7801 gcccaggatg ctgtccacgg tgatgggcgg ttctgttcct acgtcgttgg ttttcatggt
     7861 agtgggatag tatttcagta cgccagtctc gtcctgttcg atgttgtaga tgcggcggac
     7921 agccacacgg tcggagtcga tgtcagtgga ggtgtcatcc ggcgccagga cgcacagaac
     7981 cgcattgttc ttggcgatgg ggtcggattc ctgataagcg gtggcaatga cggcagtgtg
     8041 cttgtgaatg gacgctgctg cgctttcact gtctaccgtg ccggcagcat tcagatccgt
     8101 tgtgccggtg gcaactgcgg cggcacctgc ctccatggtg tcatattcca tcagatagat
     8161 gtagtggccg aagatcttcg gcgtatcttt ccgggaaaac agaatgttcg tggtcaggga
     8221 cactgcgacc accaggatca gcaggattgc cagaacgaca gcaaggatgg atttggattt
     8281 ctttttcata atgctaccta cctttttatg aagtcaaaat gatgcttttc accctattat
     8341 aacacatctt ttctgcgatt tcaattgttt tttcaacttt tttgcagaaa agtgcaggat
     8401 ctgcagnnnn nnnnnnaatg cctgtccgac tgacggcatt tttacagtac cctatcatta
     8461 tagcacatct tcccggcaat ttcaatcgtt tttttgattt ttctgagaat ttttttgaaa
     8521 cctcttgaca aacggagaat ttgtggtata ataaaatggt acagaacgtg gggtattctc
     8581 tgcgctgtac cagttgtcat aaattcatac cggtgctgga atagctcagt tggtagagca
     8641 gcgcattcgt aatgcgcagg tcgcgtgttc aagtcacgtt tccagctcca tttttgtatc
     8701 attggacggc gttctgatga tgcgagcgta gtttagtggc aaaacatcag cttcccaagc
     8761 tgaggttgcg agttcgattc tcgtcgctcg ctccagacgg cacgtatgca ggtacgtgcc
     8821 gtttttcata cccggatgct atactattat aatagagaaa atccatttcc catgccgcag
     8881 ggtgcaggaa atgcggcggt gcgccgggaa atttcctgcc tgtggtggaa tttgcacaaa
     8941 ttgtggcatt gcagcaaagg tgaaatttgt gtaaaaagca tcttgcatcc ggggaaaaaa
     9001 tcgagtatca tagataatgt gcagacctgt gccagataca ggtcgcctga acgaaagact
     9061 tccggaaagc agaatctgtt ttccatggaa agaaatgagg aaaacactta tgttaaagac
     9121 aaagaagatc ctggcattcc tgctggcatc cgcaatgatg accgcagcat tcactgcctg
     9181 cggcagctcg gaagattcca aggaatccag cagcaaggca gaatccagcg aagaatccag
     9241 tgaggagtcc agcgaagatt ccgttgagga atccgccgca gatgaagacg aaagctctgc
     9301 accgcagcag gtacagaccg gcgatttcga agaggcagtc gtaccgcagg ctggcgaagc
     9361 ttatctgagc atggctgacg gtcagtggtg gcttcagtac accggcagcg atgcggacta
     9421 tctgtgctat gacgcaggcg ttgctccgat caccggcaac ggcgaataca ctgtcagcgt
     9481 aaccgttgac accaacgctg tccgctacga tgcgaccggc gacatcaacg gcgaactgat
     9541 cgcaaacggc acttccttca tggcagtaca gatcatggac ggcgcaaccg caacaccgaa
     9601 cgcagtgatc gacgttactt ccgttaaggt tgacggcaca gagatcccgc tgaccaagaa
     9661 gagcttcacc aacaccgagg acaccgagat cgacggcacc aagcactcca acgttcgttc
     9721 caacatcttt aatgagtggg taccggacga cagcctgccg ggcgatgcaa gaagtgcaga
     9781 gggcaacatt gcagatctgg caaacaagtc cgattattct gcaactatcc tggatccgtc
     9841 tgctatcggc gactggacaa ccatcgaagt tactttcaac gtaactggta tgtaagcagc
     9901 aaaactgcat cgaatttgca gaccgactgc ctgtaccgtt ttggtacggg cagtttttgt
     9961 ttatgccata tgtgaacatt ttgaaaaata ttgcggatat gccttgcggc gtgggaaaag
    10021 atgtgctata atagaacacg tcgctgaaat ggaagcttag ctcagctggg agagcatctg
    10081 ccttacaagc agagggtcat aggttcgagc cctatagttt ccaccagtgg tctggtagtt
    10141 cagttggtta gaacgctgcc ctgtcacggc agaggtcgtg ggttcgactc ccatccagat
    10201 cgccagtgcg gatttagctc atctggtaga gcgccacctt gccaaggtgg aggtagcgag
    10261 ttcgagcctc gtaatccgct ccaaatggcg ctatagccaa gtggtaaggc aagggtctgc
    10321 aacaccctga ttctccagtt caaatctgga tggcgcctcc atgagaatga gaacgcaaag
    10381 gcgttctctt tttttatatc tcatggagac aagcctggga atgcagtccg ttgcccagag
    10441 cctgtcccgt gttcctttgg atgaatgcga ggcaggctct tatcgcacca ttgcattttt
    10501 atcggaaagg agggaatctt atggcaactt atctgatcga ctacgaaaac gtctatatcg
    10561 agggtctgac tggtctggaa cgtctttcgc agcgggatga ggtggtgatc ttctacaccc
    10621 agaaccgctg cggactgacc tttgaactgc accagcgtct gatctcctgc aaggcaaagc
    10681 tgcggctgat ggaggtgact gcgctttcca cccggaagaa cacggtgaaa aacgcactgg
    10741 atctccagct ttccatgtat atcggttacc tggtgggcag ccgtcccaag gaaccgctgt
    10801 atatcatcag caaggacaca gacttcgacc tggatctgac gttctttgac cagtacctgc
    10861 aaacgggaaa agcgtccctg gcgatctgta ccaccatcga gaatgccctg gaaggcgttc
    10921 ccgaaacgaa tgcagaacag atgctgacag agacggagga ggagacggcg gatacgactt
    10981 ctgcggaaga agaaaacgta ccggaaacac ctcggaagaa gagaaacaac tctgtggcga
    11041 tgttccccaa ggaaatgcag tccgaggttc gggagctgct ggatctggac gacaagcgaa
    11101 ctgtgaaccg catttgtgcg attctggctg tctctccgga tctgctgact ttgaacaacc
    11161 tgctgtaccg gcattgtcat gacggcaagc aggtgaagga actctatcac aagctgaaac
    11221 cgtcctatca ggaactgcgg aaactggtga atgaggccaa gtaactgctg acaaaaagcc
    11281 gccgcccggt gctgaaactg accggacggc ggcttttctg tttggtttgg ataaaaaatg
    11341 aaaaaccgaa atccggcgac aatttctatc gcagatctcg gctgactttc atattcaact
    11401 gtctaaagaa tgcttacgca cgctcgatct tgccggaacg caggcatctg gagcaaacgt
    11461 atacatgaca aggagtaccc ttcacaatcg cttttacacg ctttacgttc ggcttccatg
    11521 ttctgttaga ccgtctgtgg gagtgagata ccttgatacc aaaagtaaca ccctttccgc
    11581 aaacttcaca ctttgccatt ctactgcacc tcctcatcct agcattgctg ccaactgccg
    11641 taccggggta tagcagtcca caaactaaca tatctatttt atcaaattct gcggcaaaat
    11701 gcaagctttt tttcggattc tttgaaaaaa atattcttgt gcgtaaaatc ttaaaaaagc
    11761 ataggacaag atgcaagata gaaaaataat ttgatgtatt tgatttataa acggaatgga
    11821 ttagaaataa cctgcgttac agcatccaaa aaatcacacc gggatcttgt aaattttatg
    11881 ccgcagggca aaatacactt gaaatttgac gcaaaatcat gtataataaa aggaacacat
    11941 tgtggacggc actgcccgga gaaacggcgc agtgtggcat tgttccggat tgcagaaagg
    12001 attgaaaaca tgggaattct aagcagcttt acaaatctga accgctatga gatcctggca
    12061 cgtatcgtga tcgtgctgct ggtgctgccg ctgcacgagt ttgcacacgg ctgggtggca
    12121 cggaagtgcg gtgatgacac cgccgaggct gccggacggc tgacgctgaa tccgctggcg
    12181 cacgtggatc cgtttggtgc ggtgctgctg ctgctgaccg gtttcggctg ggcaaagccg
    12241 gtaccgatca acccggctcg gatgaaccat ccccgaaagg gcattatctg gacatccctc
    12301 gccggaccgc tgtccaatct gctggcggcg ctggtgggcg tgatcctcat gcagatcgta
    12361 tttccgtttt attataccag ctcgaacgcc gtgctgaccg gcgtgtatct gctgctctat
    12421 tggttcgcca acatcaatat ttccctggca gtgttcaatc tgatcccggt gccgccgctg
    12481 gacggctcaa aggttctgat gatgctcttg ccctatgaaa agggaatgtg gatggagcgc
    12541 caccagcagc agctctcact ggtgctgtgg attctgattc tggtcggcgt gctgaacgta
    12601 ccgctgggct acctttctta ctggatcatg aaattcttca cctgggcgac ccagtggatt
    12661 ggtacgctgg tacagatggt gatgtaactc catgcagaag atctcgttta agctggaagt
    12721 ctttgaagga cctatggact tgctcctcag tctgatttcc aagcacaagc tgaatattta
    12781 cgacatcgag atctccagtc tgctggagca gtatctggcg tatctcgacc aatgccggga
    12841 acaggatctg gaacttgccg gagagttcct ggaaatggcg gcaaggctga tctatattaa
    12901 gacggcatcc cttttgccac ggccggaaga ggcgcaaaag gagaagcagg cgctccaggg
    12961 cgcactgatc gaatatgcgc tctgcaaaca ggcggcgcag gttttggcgc agcgctatgt
    13021 gggcggaaag atctacaccc gtccgcctat ggaaattgca gtttcggcgg catacgcccg
    13081 gacgcacctg ccctatgtgc tgctggagac ctggaacgcc atgggcaaaa agcgctttgc
    13141 cccggaggac caggcacggg accgcctgca gcgtgtggta acacgcccgg tggtctctgt
    13201 catgtccaag gtggtctacg tcatgcggaa ggtctatcat gacgaacacg tgtacctggg
    13261 tgcgctttac gccgatatcc gggaacgttc cgcacgggtg gcgctgttcc tggcggtgct
    13321 ggaactcacc aagcacggca ggatcggcat tagtgaggac ggcacgtatc tctaccgccg
    13381 gacagactac cgcagaaaac acggcgcaga aaccagggca gatgcagaat ctgccgtcat
    13441 ggtatgaatc cacagaatcg gggaatgata atggaaagaa acaccagatc accgctatgc
    13501 cttatgttgg aggcggttct gttcgccagc ggcgagccgg tgacgctctc tcggctggta
    13561 caggcgtgcg aggcaccgga atccacggtg ctgacggcgc tgaaacagct gtctgcctgg
    13621 tatgaaaagc aaaagagtgc gctctgtgtg cagcagctgg gcgacagctg gcagatgtgt
    13681 acccacacgg catatgcggc agtgatcgag cgtgccatgg agaccaaaaa ggcggcaccg
    13741 ctttcggcgg cggcaatgga ggtactgacc attgtggcgt acaaccagcc ggtaagcaag
    13801 ggctttgtgg aacacgtccg tggcgtggac agcagctccg tggtaaatac cttagtggaa
    13861 aaggaactgc tgatggaggc aggacggctg gatgtgccgg gaaagcccat cgcctatgcc
    13921 accacacccc atttcctgcg ctgctttggc ttgcagtccc tggaggaact gccgccgctg
    13981 ccggtgtcgg ctgcgccgca ggaaaccgca gaagagaccg aagaaacgga cacacccacg
    14041 gcgtagcgca gaggaggcga gcggcacatg atcggatggc tgatcgtatt gggcatcctg
    14101 gcgctgctgc tgggactgct gctgtcaccg ctggtgtttg ccttagacta tgacgacccg
    14161 aaagaccgct ttttctggaa actgtcctgg ctgggcattc cgctgctctc cagcgagggc
    14221 aaaggcgtat tccggaaaaa gacgggaaag ccggagaaaa aggaacgccg caaagaaccg
    14281 gagacagaag aaaagccgga acacaccgac aaaaagggca agctaaagcg gtactggaaa
    14341 atcgcacaga acagcctttc tgtgctgccg aaaccgctgc ggcgtttgtg gaaagggctg
    14401 tcgctccggc atctgattgt gggtgtccag gtgggcagat ttgatgccaa ggaatgcgct
    14461 ctggcgtatg gtgcggcgaa tgcggccgta tacgctgcgg tgggactgct gagcagtgcc
    14521 atgcgtgtcc acctggaaca gctgacggtg cagtgtgcct tcgggcagga acgcagccgc
    14581 tgggtgatcc gtggcaggct gcacttttgt ccgctgtccg cacttctggc gctgggcagc
    14641 tttgccatgg gctatgtgat gcagaaaaac cggcaggaag atgccgagaa agaaccggcg
    14701 gcggaaaaag aacacgctgc cgcccgataa acaggaggta aaaaaatgca ggaaaagaaa
    14761 ccgatccagg atctcatggg aatcacaatg gaaaagatgc gggaaatggt ggacgtgcag
    14821 acgatcatcg gcgaccccat tgtagtgtcg gaaaaggtga ccatcgttcc ggtgtctaag
    14881 gtgtcctatg gctttgcctc cggcggatcg gatctgccgg tgaagtccaa tcccaaggac
    14941 ttgttcggcg gcggtgcagg tgccggcgtg tccatccagc cggtggcttt cctggtggtg
    15001 caggaggacg gcgtgcgtct gctgcaaatg gatgagggca gcgatgccct ttccagcgcc
    15061 atccgctctg tgccggaggt agtagaccgc ctgggcagca tcttcaaaaa ggacaaaaaa
    15121 gacggcgatg cgccggaaga tgctgaggca taaccggcgg catttcgcat gaaaccgtcc
    15181 tgtcccacat acactgtact aaaaacggga ggtattgtgt atgggcagta cattttttcg
    15241 gcggtggatc ggcggctttc tggcggtgtg tctgacgctg accggaatgc cggcgctgtg
    15301 cgtttccggg gcagagccgg cagtttcggc gcagtcctgc attttaatgg aggctgtgac
    15361 cggagagatc ctctatgaga aacaggcgca ggaacagcgt cccatggcga gtacgaccaa
    15421 gatcatgacc acgctgctct gtctggaaag cggcgatctg gacacggaat ttcccgtgga
    15481 tacggatgcc attcacgtgg aaggctcttc tatggggctg gtcgagggcg acatcgtgac
    15541 taagcgtgcg ctgtgctacg ggatgctcct gccgtccgga aacgatgccg ccggcgctgc
    15601 ggcagtgaaa ctggcaggga actatgccgc ctttgcggag cagatgaacc agaaggcggc
    15661 ggagctgggg atgacccaga cccattttgt caccccgtcc ggtctccacg acgaccagca
    15721 ctattccacg gcgtatgaca tggcgctgct gacggcggca gccatgcaga atgagacgtt
    15781 ccgggagatc tgccgccagc cctctgcaaa ggtctgtttc gggaattcgc cgtatgagcg
    15841 gtggctgacc aattccaaca agctgctcac catggatgaa acggtggtcg gcgtgaaaac
    15901 cggattcacc gacgaggcag ggcgctgtct ggtgtctgcc tgtgtgcgaa acggcattat
    15961 gctgatctgc gtgacgctga acgaccggga tgactggcag gatcacgagc gcctgtacga
    16021 ctatgggttc gggcagctga cacccacaga ggtttccttt ccgacggact ggagcattgc
    16081 tgtggctggc ggaactgctc agcaggtaac actgattccc gaaggcacca tcgtggcagg
    16141 gacaagggac ggaaaagccc cggcgctgga gtttcagctg gagactgcgc cgtttctcta
    16201 tgcgccggtg actgccggac aggatgccgg aacatggaaa gcgctctgcg gcggcagagt
    16261 tgcggcgcag ggcaggcttt gcgccggaga aagcgctgta tatcagaaaa aatccgcagc
    16321 accgcagcag cagagctggt ggaaacggat gctgcaaaag ataaaaaagg agaagaaccg
    16381 gaagtggaaa aaattcgaat tcaaaagctg ctggcagatg ccggctattg ttcccggcgg
    16441 caggcagaac agctgatctc tgccggaaaa gtaaagctga acggtcaccc ggtcaagctg
    16501 ggggacaagg gaacgctggc agacctgatt accgtcagcg gtgaacgggt gtatctgccc
    16561 agaaagaaac agtatcgcta tctgatgctg tacaaaccac ggggctatgt gaccacaact
    16621 tctgacgaac tgaaccggcg gtgtgtgatg gatctgctgg aggacgtgga agagcgtgtc
    16681 tacccggtgg gacggctgga caaggactct gagggactgc tgttgctgac caacgacggc
    16741 aacttcgcca atggcatcat gcaccccagc cgccatatca gcaagaccta tcgtgtcacc
    16801 gtccgtccgg atgtgaccga agaacagctt atcaccctgt ccagcagcat ggagctggac
    16861 ggcaaaatga cccagccggc aacggttgtg gtaaagacca aagagccggg atgtgtggta
    16921 ctgcttatga ccattcacga gggcaagaac cgccagatcc gccggatgtg tgaatccgtc
    16981 gggctggaag tggcacgtct gcgccgcacc agcatcggtc cgctgaaact ggggatgctg
    17041 aaaccgggta cttatcggga tctgaccgca gaggaactgc gtgcgatccg caacgccata
    17101 ggtgccggcg agcaggcatc gaccgcaaag ggcaagcgcc gataagatac gcaagctttt
    17161 tgcaaggcta cccttgaaaa aattctgaga ggaggaacag gcaatgccaa tttgtatttc
    17221 accggatctt ccggcatttc acattctgtc cgaggagaac atcttcgtca tggacagcga
    17281 ccgtgctgcg caccaggaca tccgtccgct gcgggtgctg gtgctgaatc tgatgccgaa
    17341 aaaaattgaa acggaatgtc agttcctgcg gctgctcagc aacacaccgt tacaggtgag
    17401 tgtggaattt ctgcacgtct ccagccatat cagcaagaac acatcccaga accatctcgc
    17461 cacattctac cgaacttttt cggagatccg ggatcagtat tatgacggct gcatcatcac
    17521 cggtgcgccg gtggagcatc tgaaattcga ggatgtggac tattggaacg agatctgcga
    17581 cattatggag tggacaaagt cccacgtttt ttccaccctg cacatctgct gggcggcgct
    17641 tgccggactg tattatcact tcgacattcc caagtatgac ctggcgcaga agatgttcgg
    17701 cgtctttccg cagcacgtgc tggaacccca ctgccagctg ctccgtggct ttaacgatgt
    17761 gttccatgcg ccccattccc gtcatgcaga ggtgcatcgg gaggacatcg aaaaggtgga
    17821 acagctgaag atcctgacgg aatcgccgct gtccggcgtg catattgtgg caaacaagaa
    17881 cggcagacag tatttcatca cgggacattc ggaatatgac cgggatacct taaagggcga
    17941 atatttccgg gacaaggaaa agggcgcaga catcgctctg ccctatgact atttcccgga
    18001 cgacgacccc accaaggagc cgcactacag ctggggatgc agcgctaacc tgctgttctc
    18061 caactggctg aactactgtg tataccagca gacaccgtat gatctgactt ccctggaact
    18121 gtacaactgg gactggcagg caggtctctg atgatcgcat agaggagcgc ttgttgatgc
    18181 acaagcatat ccacagaaac agaaaaaccc cgtccgcacc ggagagcctt tggtgcggac
    18241 ggggttcgtt atgttatctc atttctgtac acgcattgca gaagtgggat ttctttttta
    18301 gaggtatgtg aggaactggg atgccagaac
//
LOCUS       DAWENO010000022        18030 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1293_length_18030_cov_12.160223, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000022 DAWENO010000000
VERSION     DAWENO010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1293_length_18030_cov_12.160223"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3506..3515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4561 a         4539 c         5196 g         3724 t
ORIGIN      
        1 atgtaagaac atggagtgtc ttgcagcagc cagaaaggga caccgcatcg agcgctcttt
       61 tggctgccgt gtaccggaag aagtgtacga ggagatggaa aatgcactac aggcagaact
      121 ggcaggggat tctgagcatc tgtcgtaagg caggaaagct tgccatgggg ctggaaccca
      181 caaaggatgc catgtaccgt ggcgaagtct ttggcgtatt ggtagcgtcg gatgcgtccc
      241 ccaagacgca gaaagaggcg gcgttctatt gcagccaggc agaagtgcct tgcctgcagc
      301 tggacttcac caaggcagat ttcgcccaga tgatcggcag aggcagcggc gtgctggcgg
      361 tgtgcgatga aggatttttc cgtaagatgc agaccatcgc cgcaaaggaa aacgcctcgc
      421 cggaaaagtg aaaattgttc ccggagcaat ccgggtaaat agacagcgct gccggaaacg
      481 ggcggcgtgt gcagacgcag gtttggaatg cagcggaaga acaggcggtt ctttcgggcg
      541 ttccttggag aaaaggagga ttttcgccta tgacgaagaa agtgaaactg agtgatgtag
      601 cacgggattt acagatctca acacaggaac tgatcggatt ctatcaggaa cgggatgcca
      661 acggcaacac caagaagggt gtgaccggtc tgacaccgga agaaatcaac ctggcactgg
      721 agtattattc tcagaaatct caggtagaga gcctggatgc gtattttgca tccaaggaca
      781 ccccgaaaca gaaacaggag aaaaagccga gcgagaagaa accggcagaa aagaaggcat
      841 cttccgagaa aaagcagtcg gaaaagaaac agccggataa caaaaagcag cagaaacccc
      901 aggctgcgga aaagaaaccc caggcacaga aatccgaaaa gccggcagaa aagaaaccgg
      961 cagctgcgga aacagctaag cccaagcaga acaatgcgcc gaagcatgaa aagcccaaaa
     1021 agcagcaggc aaaggcacag aaacacggcg agcgtctcca tgtagaagtg gagatggcat
     1081 ctgcggacag cattaccaca gaaaagcgcc gcactgtcga tacccgtggc tcttatgtag
     1141 atctggataa gtataaccag cgctatgagc agattgctcc ggcaaataaa tacaccaacg
     1201 acaagtattc caccaaaaag cagaagatca accagaagtc tgcacagcgc aacaagcagc
     1261 gctattccaa caagcgtgag acagagcagg ataagctgcg ccgtctggag ctggagcgtg
     1321 ccagaaagca gcagctgcgt gtccgcatcc cggacgtgat ctctgtgggt gaactggcga
     1381 cccgtctgaa agttactgcg accgaggtca tcaaaaagct gatgatgctg ggcgttatgg
     1441 ctaccatcaa tgaggaaatc gacttcgaca ccgcatctct ggtggcagag gaactgggcg
     1501 caaaggtaga acacgaagtg atcgtgacca tcgaagaccg tctgatcgac gaggatgacg
     1561 acgccgagga taccgaagag cgctgcccag ttgttgtagt tatgggtcac gttgaccatg
     1621 gtaagacttc tatcctggac cgcatccgga atgcacacgt taccgcaggc gaggctggcg
     1681 gcatcacaca gcatatcggt gcataccagg taagatatga gggcaaggac atcacattcc
     1741 tggatacgcc gggacacgaa gcgttcactg ccatgcgtgc aagaggtgca aacatcaccg
     1801 atattgccat cctggtcgtt gccgcagacg acggcatcat gccgcagacc gttgagtcca
     1861 tcaaccatgc aaaggctgcc ggcatcactg tgatcgttgc catcaacaag atggataagg
     1921 aaggcgcaaa cccggatcgt gtcaaggaag aactgaccaa gtacggcatg gtatgcgaag
     1981 agtggggcgg cgacgtgatc tgcgttccgg tttctgcaaa gaccggcgag ggcatcgacg
     2041 aactgctgga gaacatcctt ctggttgcag agaccagcga actgaaagcc aatccggatc
     2101 gccgtgcaaa gggtacggtt gtggaggctc gtctggacaa gggacgtggc ccgatcgcaa
     2161 ctctgctggt acagaacggc acactccaca ccggcgatgt cattatcgca ggcactgcgg
     2221 taggccgtgt tcgtgttatg accaacgaca agggcaagac cgtgcatact gccggcccat
     2281 ccgttccggt agagatcacc ggtctggcag aagtgccgga ggcaggcgat gtattcaatg
     2341 ctgttgaaga tgagcgtctg gcaagagaac tggttgagca gagaaagcac gaggcaaagc
     2401 aggcgaagtt caacgaatac cagaaggtaa cgctggataa cctgttcagc cagatcgagc
     2461 agggcgaaat gaaggaactg tctctgatcg taaaggcaga cgtacagggt tctgttgagg
     2521 cagtgactca gtccctggaa aagctgtcca atgaggaagt tcgtgtccgt gtgatccacg
     2581 gcggcgtcgg cggcatcaag gagagcgacg ttatgctggc gagtgcatcc aatgcgatca
     2641 tcatcggctt taacgtgcgt ccggatcaga ccgcagaaga gatcgcagcc cgggataagg
     2701 tagatatccg cacataccgt gtcatctatg atgcgatcga agagatcgag accgcaatga
     2761 agggtatgct ggcaccgaaa taccgtgagg tcgttatggg acgcatcgag gtgcgccagg
     2821 tttataagat ctccaatgtc ggtgcagttg ccggtgctta tgtgctcagc ggcaaggtca
     2881 ccagacagtg cgagatccgt gtggttcgtg acggcatcgt gatcgctgag gataagatgt
     2941 ccagcctgaa acggttcaag gatgatgcca aggaagtcgg cgaaggctat gagtgcggta
     3001 tcactctgga gaagttccgt gacttcaagg aaggcgacat cttcgaggcg ttcatcatgg
     3061 aagaatacag agactaatcc gatacagaca gcacttcgga agaggattcg gaggatgtgc
     3121 tgtttatcgt ttacagaaag gagccgtaca gcatggcaaa cttcaaagca aaccgcaccg
     3181 aagaggatat cctgcgggaa atgacagcga ttttgcggga actcaaagat ccacgcatcg
     3241 acccgatgct gacggtggtc cgtgcagagc tgtcccggga tctttcggac tgcaagatct
     3301 tcgtctccag tctttccggc aaggatgccg ccaaggaatc ctgcaaggga ctggacagcg
     3361 ccgccggcta tatccggcgt gaactgctgc accgtctgaa gatccgccgc agcccggtgc
     3421 tgcactttgt ggcagatgat tccatcgcac acagcgcaga gatcgaccgg atgctgcacc
     3481 agatccagcc ggcagtggaa gacctnnnnn nnnnnaccgc agcccggacg gagactgcat
     3541 cggcgccgga tatgcgctgg cagagatgct gcaccagctg ggcaagcgtg ccgtggtgct
     3601 gtgcaatgac gagatcccga agcgatatca gtttatgctg ccggaagagg acggcacagc
     3661 ggcgcatttt gaaccgcagt gcatcattgc cgtggatgtg gcagacgcca agctgctggg
     3721 cagcagcgtc caggaacact acggcagccg ggtagacttg tgcatcgatc atcatgtttc
     3781 caacgtccct tatgccaagg cacgtctcct ggatggtgcg gcatcggcgg cgtgtgagat
     3841 cctctatcgg ctgagccgca tccttccggt gaaaatgacc gatcatatgg cgctgtgcct
     3901 gtataccggc atggcgacag acaccggctg ttttcagtat gacaatgccg gtccggaaac
     3961 ccaccgggct gtggctgcgc tgatggagat gtgtccggat gtgcgctatg cgtggatcaa
     4021 ccgggagatg tttgccgtga agagtttcgg cagactccag ctggataagc tgcttatcga
     4081 ccatatggag cgctatctga atgacaagtg catcctgatc tgcgtctccc aggagtttct
     4141 gcggaagttc caggtggatg aggcagagct ggagggcatc gccggatttc cgctccaggt
     4201 cgaaggcgct gagatcggca ttaccatgaa ggaaaaagaa cccggcaagt ttcgcatttc
     4261 catgcggtcg gcggataagg tcaatgtatc cgccatctgc cagcgcctgg gcggcggcgg
     4321 tcatgtgaag gctgccggat gccaggtctg cggcaccgct gacgaagtgc gcaacatcct
     4381 gctggatgca gtgcaaaaag aatggagcaa cgcatgaatc tggacacagt gcagggtgta
     4441 ctctgcatga ataagcccac ggaattcacc tcctttgacg tgatcggcaa gctgcgtggt
     4501 attctgcata tgcggcggct gggacacacg gggacgctgg acccgatggc gaccggtgtg
     4561 ctgccggtgc tggtgggaaa ggcaacacgt gcctgcgata tcctgccgga tcagaccaag
     4621 tcgtatcgtg cggcggtaca gttcggctgg gagaccgaca ccgaagatgt gtggggaatc
     4681 cggacagcgg cgtatccgga gatgcacgtg acaaaggaaa tgctggacga agtaattccc
     4741 cggtttctgg gagagatcca gcagatcccg cccatgtatt cggcggtctc cgtcgaggga
     4801 aagcggctct atgagctggc acgaaagggt gtcgtgatcg accgcccggc gaggacggtg
     4861 caggtggatg acatcaccct ggaagaatgg gacgaggaaa agcagacggc ggtgctgtcg
     4921 gtgacctgcg gcaagggaac gtatatccgt accctgctgt cggatatcgg cagggcgctg
     4981 ggcggcggcg ctgtgatgac ggcgctctgc cggacgatgg cgcatggttt cacactggaa
     5041 cagtgctata cgtttgagca ggtgacagag ttctgccgga tgggaacact ggcagagcat
     5101 ctgatcccca ccgaccggct gtttgcggcg ctgccggcac tgcatctgac agaacagcag
     5161 accgctcttt acggcaacgg cgtgaaactg gcgctgagcc gtgtggaggg catcctgccg
     5221 gatcaggaac aataccgggt ctacggcgca gacggctgct ttttcggcac ggcgcagacc
     5281 gaccggaaaa gcgacgaact gcgtgtggga aagaatatga agtagaaagg cggaaacggg
     5341 cttggaaaag ctgaggcgca tcactgcacc gctggcacag gaaagtgccg tggcgctggg
     5401 attttttgac ggtgttcatc tgggacaccg tgctgtgctg ggtgctgtgg cacaatgtgc
     5461 ggcggcgcag gggctgatgg cgtgtgcatt cacctttgcg gcagattccg tcccggtcaa
     5521 gcagggcgtg ccgctgacct acctttatac cgatgcccag aaagcggaac tgatgcagga
     5581 ctgcggcatc cacggcgtat actgcccggc gttttcggaa ctctgcgccc tggacggcga
     5641 atcgttctgc cggcaggtgc tggtggaact gctccacgcc agagaggtgt tctgcggcgg
     5701 tgaatttcgg tttggcgcaa aggcggcgtg gaacatcgac agtctgcggg aattcggcag
     5761 gcagatgggc tttgccgtgc atcagatcga cccggtgttt tgcggcggcg agaaaatttc
     5821 gtctacggcg atccgtgatg cgatccgtcg gggcgagccg gagaccgcag ccggaatgct
     5881 ggggacggcg taccgcatca gcggcagcgt tgtccacggg gcagcgctgg gacgcaccag
     5941 ggcagtcccc accatcaata ttccctttgc accgggacag cttgtcccca ggtacggggt
     6001 ttatgtttcc cggacgcaca cgccccaggg cagttttgat tccatcaccg atgtgggcgt
     6061 aaagcctacg gtggcagatg cccaggcacc ggcggcggag acatttctgc tggatttttc
     6121 cggtgatctg tacgaacagc cctgccgggt ggagctgctg cattttctgc gtgacgaacg
     6181 aaagtttccg gatgtggaaa gcctgtacgg gcagatcgca cgggatcagg agcagtgccg
     6241 ggaatggctg aagaatcata aaaacctata agagcagaca actcttttct gaatagagac
     6301 agcggtgctg tcaagacaaa aatgcgaaag gaagttcaaa gcaatgaaca agcgagtaag
     6361 aaccagattt gcgcccagtc cgacgggcta tatgcacatc ggcaatctgc ggacggcact
     6421 gtatgcctat ctgattgcca agaaacagga cggcgatttt attctgcgaa ttgaggatac
     6481 cgaccaggag cgctatgtag aaggcgcagt tgacgtgatc tatgacaccc tgcgtgttgc
     6541 cggtctgaac tgggacgaag gcccggacat cggcggcccg gttggtccgt atgtgcagtc
     6601 tgagcgcatg ggaatgttca agtcctatgc ggaagaactg gtcaagagcg gccacgcata
     6661 ctactgtttc tgtgacaagg agcgtctgga cgaagtgcgc aagatccagg aggcatcgca
     6721 cattgcaccg atgtacgacc gccactgccg tgacctgtct ccggaagagg tacaggcaaa
     6781 gctggacgca ggcgtgccgt atgtcatccg ccagaagatg ccgctggacg gcacgacgac
     6841 cttccacgat gacatctatg gcgatgttac cgtagaaaac agcaccctgg acgaccagat
     6901 tctcatcaag acagacggaa tgcccaccta caactttgcc aacgtagtag acgaccatct
     6961 gatgggtatt acacacgtgg tacgaggtaa cgagtatctg tcctctgcac cgaagtacaa
     7021 cctgctgtac caggcattcg gctgggatgt gccggaatat gtacactgcg caccggtcat
     7081 gaaggacaag acccacaagc tgtccaagcg aaacggcgac gcatccttcc aggatctgat
     7141 ggcaaagggc tatctgcccc aggcagtcat caactttatt gcactgctgg gatgggcgcc
     7201 gtccggtgag tataacgagc aggagatctt ctccatggac gaactgaaaa aagcgttcga
     7261 gatcagcggc atcagcaagt cgccggcgat ctttgatgac aagaagctcc gtgccatcaa
     7321 cggcgcatat gtgcacaaga tggatccggc agagttcgcc aaggcagctg agccgtacat
     7381 ccgtgagacc gtaaagcgca cggatatcga catggcactg ctgtgcagcg ttctgcagaa
     7441 ccgtaccgaa gtgttttcgg atattccgga gcaggtagac ttcattgacg caatgccgga
     7501 gtacgatctg gagatgtacc gtcacaagaa gatgaagacc acgccggaga ctgcactgga
     7561 tgcactgaag gagacgctgc cagtgctgga aggcgtaagc gagtgggatg tggagcatat
     7621 ccacgaggca ctgttcgcaa agatcgcaga cctgggcgta aagaacggct ggatgctgtg
     7681 gccgctgcgt accgccgttt ccggcaagca gtttacaccg ggcggcggcg tagagctgtg
     7741 tgcgatcctg ggcaaggagg acacactggc tcgtatccgc aaggcaattg atgcgctctc
     7801 cgcataagat tgccatcttt cgtaagacta tcacgtcatt atcacaattg gatgataaac
     7861 tatagctagt ttttgtgtgc cggagcgccg ggattttttc gtttcggaga gaccgcaggc
     7921 tcggcataca gcagaaggag gcaatcacag ggatgattga agtcaaacat ctgacgaaac
     7981 aatacgggga taagctggcg gtcaacgaca tcagctttac cgtagaggac ggtgagatcc
     8041 tgggacttct gggaccgaac ggcgccggaa agtccaccac catgaatatg ctcaccggct
     8101 atatcagctc cacatccgga caggcactga ttaacggcat cgacattctg gaagatccga
     8161 tcaaggcaaa ggcacagatc ggctatctgc cggagctgcc gccgctgtac ctggatatga
     8221 ccgtcatggg ctatctgaac tttgtctatg acttgaaaaa gtgcaagctg ccacggcgct
     8281 cgcatttgaa ggacgtctgc aatctctgcc ggatcagcga cgtttccggc agagtcatca
     8341 agcacctgtc caaaggttac cggcagcgtg tgggtctggc acaggcgctc atcaacaatc
     8401 cgccgattct gatcctggac gaacctaccg ttggtctcga cccgaaacag atcatcgaga
     8461 tccgtaccct ggtgaaaaag ctgggcaaaa agcacaccgt gatcctgtcg tctcacattc
     8521 tgaccgaggt gcaggaagtc tgcgatcgtg tggtcatcat caaccatggt cagatcgctg
     8581 cgaatgacac cattgacaac ctgtccaagg cagtttccgg tgtcaatcgt ctggtggttc
     8641 gcatcaacgg accgaaaaac gaggtcctgc aggcgattcg tgggctggaa gatgtcacca
     8701 aggtgcgtgc cgacatggaa cgggaaccgg gcatcttcga attcgagatc gaggcaaagc
     8761 cggacgcaga cattcgcccg gcgctgaacc agatggtcga gtcccacgga tggtacattt
     8821 ataagatgga tctgggcgta atgacgctgg aggatgcgtt cctgaagatc actatgggcg
     8881 agggcgtaaa gctgtccgcc gccaaaaaga gtgaaccggc agcagcatct gcaccggaga
     8941 aaacagaaac accgcagcag gaaactgcac cggcagaaac ggaaaacgaa cagccggcag
     9001 atgcggcaga gacaaaagaa ggaggcgagg cgtaatgggt gccatttatc ggcgtgagat
     9061 cggggcgttt ttcacctcca gcattgccta tgtatttctg gcagtgttct atctggcagc
     9121 aggctacttc ttctttggct catccctggc gatgtctacc acagatatgt ccaacctgtt
     9181 caccagtctg ttctttatcg taaccattct gattccggta ctgaccatgc gcttgttcag
     9241 tgaggaaaag aaacagaaaa ccgagcaggg gctgctgact gcaccagtgt ccatcggcgg
     9301 catcgtcatg ggcaagtatc tggcagcgct caccctgtac accattggcc tggcgatcat
     9361 cttcgtctat gcgctgatcc tgagcttttt cggggcagtg gcatggggta tcgtgatctc
     9421 caacattctg gcgctgttcc tgctgggcgc agcatttatc gcagtgaccc tgttcatctc
     9481 gtctctgacg gaaaaccagg tcgttgctgc ggtggtaggc atgatcgttc tgctggcgct
     9541 ggcactgatc gacgtggtgg ctcgttatgc agagagcgtt ccggtagttc ccacagtgct
     9601 gaatgcgctg tcgttctata ccagatatta tgattttact gcgggtctgt ttgatccggc
     9661 cagtgtgctg ttttatgtca gcacggcagt gatctttaat ttctttactg tacgtgtgtt
     9721 cgaaaaacgg cgctggagct gagaggagga catcacccat ggaaaatcag aaaaaggaaa
     9781 aaacaaccga ggcttctctg gaaaaacagc agaaaaagct gaaaaaccgg aaaaagctga
     9841 aatacggcgg tctggcgact gccattaccg tgatctttgt tgcagttgtc gtattggtca
     9901 acgtggtcgt tacccagctg gataagcgtt ttccgaacgc aaagttggat ctcactaccg
     9961 caaacctgta tgagatcagc gacgagacac tggactacat ccgcaacctg gataaggatg
    10021 tagatgttgc catctcttcc gatgaggcga catttacctc tgacaaatac aacaagatga
    10081 tcgctgaaac actgtccaag taccagggct attctgaccg catcaatgtc acctattttg
    10141 acaccaccaa ggatccggat gtgctgtcca agtaccagga actgtacggc ggttcgatcc
    10201 agtccggaca ggtcatcatc aacagcggca agcgtatcaa ggtgtacaac acccagaccg
    10261 atatgtttga agtcgatcag cagaagtatc agtactatca gtacggtatg gcaagcttct
    10321 ccgactgcat caccgcattc aagggcgagc agacgctgac ttccggcatc atgaatgtga
    10381 ccgactccaa ccccaagacc gtgggcattc tggcgaccag caacggctct cccatcttcg
    10441 cacagaccag ttccagtgca gaccccaaca cctacgcatt ttatgctatg gaaaatctgc
    10501 tggacgaaaa cggctatgat gtcaagcgtc tggacatgat gaccgatgag ctggacactg
    10561 cgacctatga tattctggtg ctgcctgcgc cgaaaaccga cctgaccatg gattccatcc
    10621 agaaactcca ggacttcatg tataatgacg gcaacctggg caagcagctg gtttacatcg
    10681 cagactacac ccagtcatcc accccgaatc tggacgcttt tctgaaagag tggaacgtac
    10741 aggtggacta cagctctgtc attgacgaga acaacagttc caaccagcag gtaaacatcc
    10801 tgctgtccca gaacagcaac agcagctttg ttgccccggt cgtaaccgta acggacgacg
    10861 aggactataa cggacatctg gcaaacacca gcctgcctat cgttgcaccg atggcacgtt
    10921 ccatccagac actgactgcc aacaacggca gaaccgtaac atccctgctg acctcctcgg
    10981 agacctctta ctgctatccg ctttcgccca agacttataa caccgacagc acagacagca
    11041 ctgcggcaga cggtgagacc caggcggcaa cggaggcagc gactgaggct gcaaccgaga
    11101 acaccactac cacaacttcg tttgacaagg acgcagcacc ccacggtgca aacaccgtca
    11161 tggcgctgtg ccgtgaccag cagtccaccg gcgattcgtt catcgagagc gatgtgatcg
    11221 tgctgggttc tatgtccatg ctggacgcta acctggtgca gaactcttcg tacaacaacg
    11281 cagaatattt cgtcggcctg ctgaactctg tttgcggcaa ggaagacagc atcgttgtgg
    11341 catccaagga tctgacacag acttccatca cggcaacagc aacacagctg aaagtgattc
    11401 gtctggttgt cgtattcctg atcccgctga ctgttgtggt tgtcggcgtg atcgtggcag
    11461 tccgcagacg ttatcgttaa ccgaaaggat ggagcatcat gcgtaaaaaa acgctgggaa
    11521 tcatcggcgg cgtcgtattg gtggcagcac tgggaacggc atcgtttttc ctgctgaaaa
    11581 atccgccggc agaggagtca tcctcttccg cagtatccga tgaggacagc agcgagagca
    11641 tttcgctgac ctcagaggat cccaacgatg tcacctcaat tgatattaca aatacttccg
    11701 gaagctttga agtggtccgc accgcagagg gcagcggcac cggagatgac catgcggcat
    11761 atgccgtttc cggctgggaa gatcttccca tggacacctc taccctgtgg acgctgtcca
    11821 acaacaccgc atctatggac accaccggcg ttgtggagga aaactgttcc gatctggcga
    11881 agttcgggct ggacgatgcc gaggcgaccg cagtgaccct gcacttcgcc gacggcagcg
    11941 actacagttt ccgtgtgggc aatgcggcgg caaatgcaga aaacacctat cttgcgccgg
    12001 cggacaaaga cactgtctat acggtaaaaa cttccctggt ggcgaatttt agcaagagcg
    12061 ccgaggactt cctgtccaaa accatgctgg aagcgccggc agataccaac gattatccca
    12121 ttgtcaacaa gctgaccata gaccgcaagg acatggacta taccctggaa ctggactacg
    12181 acgaggcagc cgcagacgat acatccatgg gcggcatggt ggcaagccac gagatggttt
    12241 cgccggtgcc ggcatatctg agcgtagacc gttccactcc ggtggtgacg ggaatgttcg
    12301 gactcaaggc ggaaaaggta gctgtaccgc atccctctgc ggaggatatc gccaaggcag
    12361 ggctggacga cccgttcggc acggcgacta tggcgtgcga ggacaacaac acctatgtac
    12421 tgacattcgg cgaacgcttt accgaaaagg atgaggagag cggcacagag accgcatact
    12481 actacgcaat gctgagcggc gtggatgcga tctaccaggt gacggaagaa aacatggtct
    12541 gggcgacaac aacgcccacc gacattgcct ccaagctggt tttcggcact tacgtctggg
    12601 atgtaggtga gctgaacgtg agcgtgggcg aggagaagtt ccagttcaat gttgtgggca
    12661 gcgataagga taccgctgtg gtaacgctga acggcgacag caccgagtcc gaccgctacc
    12721 gtcagttcta ctcgttcctg ctgaacacca cggcggagac ggtcaagctg gacggcacgc
    12781 cgagcggcga tatgctggcg gagatcagcc tcaagaccca ggacggcaca ttcgaacggg
    12841 atctgcagtt ctatgccatc gacgatttca cctgtctcat caccgtggac ggaaagtcgg
    12901 cgtatacctg ccggaagagc tatctggata ccctgctgaa caatatgaag atctacagcg
    12961 acacaagcaa agaatttacg acaaactgga gttaaggaaa cattggtttc atccgtgttg
    13021 ccttgaccgt tccggagaga tccgggacgc tgccggagaa aggaaggaac agcaatgaat
    13081 caggaatttt ttatgtatcg tggatatccg ctggtacgca agggaaatga aatctattac
    13141 gggtatatgt cagagccatt cgtggtaatg atgcagatcg tgcatcagca ggaagtcaac
    13201 gggctgaagg ttgcagataa gattcgtgtc tatcaaatgg caaccaagga accggatcct
    13261 gtgaaggcga tcaccaagac ttccgaccga ccgaatctgt atgaggcggt agatctggca
    13321 aacgtctggc tgaaacgtgc tgaaaaggac gtagcagcaa agtaaaggca aaataaatcc
    13381 gatttgcttt caaaagagca tctctgcacg ggagtgatcc tgtgggagat gttctttttt
    13441 tagcctccct ttttcaaaaa taggctccct tgaaaaggcg cccgtagggt gtgccttgtg
    13501 ctgagctggc agccgtaggc tgactgaggg attttgtgta atctgaaaat taagggaaat
    13561 tattatgcca gaatccctcc gagcggcttt gccgcccacc tcgtactgag cggtcccctt
    13621 tgtcacctac ggtgacattt ccccaccctg tggggaatca cccttttcaa gggaggcttt
    13681 gcgcaggata gcctataaga aagaaaaatg gatttgcatg atattctttc aatttcccct
    13741 tgcatttttc tgcaccttat gttataatat gaaatgtaat tttttgatat gacaaattta
    13801 ggaggatgta acatgaaatt gtggagaaaa ctgctggcgg cagtaactgc cggaacaatt
    13861 tgtctgggga gcatcggggc aatgctgtca gtcagtgcgg aaacagagtt ggattcttcc
    13921 tccatatccg aaaatgtcgc agaggtaaag gcgtcagagg gagcgcatac gcattattat
    13981 ggggaatata gcactgattg ggatgaatgt gatgggtggt attcatttgt ggttcataat
    14041 gatggcaatg gagcatactc tttgtttggc gatgttagag cttgccaata tgcaattgac
    14101 catggatatg ttccgaatga aggagatgaa gcgtatggtg ctttttggtt tggaaacaat
    14161 ccgcccacaa taacgactac aaaagtcacc acaaaagcaa ctaccaaaac caccaccact
    14221 accacccagc cgagtgtccc caccgtcaaa gagggcagtc tggacaaggg tgatattgac
    14281 ggcagcggca gtctggattc gacagatgtg ttctactcca tgctgtacat cgccaatact
    14341 gcggtaggga ataccgtcaa aatcagcaag gaacagatca ctgccgcaaa cgtggacgaa
    14401 agcagcagcg tagatgcgac tgatgtctat tatctcatgt actacatcgc actccacggt
    14461 attggcatag acgtgtcctg ggacgacgtt ctggcaaagt aaaatggaaa gcatatcctg
    14521 aaaccggctc tttgaaaaag gagtcggttt tttatttagc ctcccttgaa aagggtgatt
    14581 ccccacaggg tggggaaatg tcaccgtagg tgacaaaggg gaccgctcag tacgagggga
    14641 accagcgaag ctggtggagg gattttagtg ataagttata gatttcacag aaaagtttcc
    14701 cagtcagtct acaggcgcct tttctgggaa ttgtagttta ggttatgatc cccctgtccc
    14761 ttcgggacat cccccttttc aagggggact taatttgctc tcgttttctc aaaaaatccc
    14821 ttgacagcac cgccaaaatc cggtataatg aaaggcaaca ccgcacagaa tgtgcaaaaa
    14881 catcaccgaa aaggagaata tacatggaag tttaccgcat tcatatggga aaggcgaagt
    14941 gcgccgttga tctgggagac ggcagtgagc gtggcgagta tgtcagccag gactatatcc
    15001 tggcgaaaat gggcagaccg caccggagca tcagtctcat gtactgctat tatcccctgg
    15061 acaagggctg gccgggccgt gccagcgtgg tacacgccag ccccgatgtc agctttgcct
    15121 gggactatcc ctacgacgac tatttcacct ataaaggcgg actgggcggc aataccgacg
    15181 gcgaaccgtt caactatatg cgtgagatcc ggcagcacgg acaggatgtt tcgctgacgc
    15241 tgaccatcga cccccacgtt tccgacgaac atctggctgc catcggcgag gatctgaaac
    15301 ctttcggcag agtgttcctg cgcatcaacc atgaggcaac gggcaactgg ttctcgttca
    15361 acaagcgctg cacctaccag gaagtggcag actttttcgt ccgtgccagc cgcatcatca
    15421 aggaacacgc accgaacgtg cagaccatta tctgcattgg cggcatcgag gatctctcca
    15481 aggaagagat ggaaaaagaa actgagttcg cacagaccat tccggcagcg gatatctggt
    15541 cagtggacaa gtatatggcg ctccactggg gctggcccta tgacgtggca gaacacggcg
    15601 gcacgactca cagccgtggc actgtggcag atacctacga aaagaccaag cgcagctttg
    15661 aacggttccg ctatctcaac ggcggcgtgg caaagcctat gaccatggca gagttcaacg
    15721 ccgacggcga tgtgaccgga ccttatgaac aggcagacat ggtaaaggaa ttctgcgata
    15781 agctggagtc ggaacaggcg gactggttca gcgctttcac attctatcag ttccgtgacc
    15841 gtggcagact gggtctggag atcgaagacc ccaacaatgc cgatgtgggc atcgcacagc
    15901 cggtgctgga cacctataaa gagatcatcc agcgtgactg gttcaagccc cagatcacac
    15961 ggcaggaggg cgaagtacag ctgccggtga cactgcgctg gagcaactct gaggatgccg
    16021 agggcctggc gattccgctg cacttcgaga agaaccccac gttctgcgag gtgaccttta
    16081 ccgaggaaag caatctgatg ctggaactga acggcaagtg gttctataaa gcccccaaga
    16141 ccaagaccgt agatctgatg gcggcgttct tcgaaaagcc cctgcagggc gaggcagata
    16201 tgacgctgaa aatttttgcg ccgccggcaa ccggcgagaa cgacctgtcg gcagaggatg
    16261 gactgtacca ctgcgacgcc gttctccacg aactgcccca gatccgcatc cgttttgcac
    16321 cggtagaggc ataaaccggc ggattctgac aggagcatat tagtgctttt tccctgcaaa
    16381 aatacccctt gacaaaaagt tgtgaatcat gtataattta agtaacgata tgaaaactgg
    16441 aattgtaaga gatgatttga aaagatgaaa taccatctcc aaaaaaaggc gattccggag
    16501 aatggagcta tagaatatgg gactttttga caaggtaaac caggtaagcc gcagcgtaag
    16561 cgaatcctcc aagagtgctg ctgagaccag caatctgaag aagaagatcg cttatgaaaa
    16621 ggagcgtatc caggaagtta tgactgagat cggcagactg ttttatgaga atccgaacgg
    16681 cgatcataag gcagagctgg aactgtgtgc agacatcgac gacagaaagc gccgcattgc
    16741 tagtatgcag ggcgatttga gttctctgaa gggtgttcgt atctgcggaa agtgcggcgc
    16801 tcggtttgat gagaagtata cttttggttt ctgcggcaac tgcggcgcaa agctggacta
    16861 aaggaaacga tgaaacagaa aagaacggca cagcctcttg caggatgtgc tgtttttgtg
    16921 tgaggagtgg aaaataaaat ggaaacaatg ctttcttttg actggaagag tgtcagtgtc
    16981 aattctgctg aggtgattgc tctgatgcgg aaacaccgga atgaaaagct ggatgtgctg
    17041 tttcattatg cagacggact ctatcgggtg ggtgtgaacc cggaaaacgc cagagaaccc
    17101 ttttacatgg atgatgagac ctatccgtcc atgtttgcgt tttgcagttc tgcctgcatc
    17161 gaaggcggct ttctgctgcc tgacctccag gcaccgctgc atatcctggc agtgaatgac
    17221 ggtgatccgt ccgcatattt tgcatagaaa gtgtaggaaa tcaacatgga aaagaaaccg
    17281 aaaaacaagt ggctctcctt tctggctgac ggtatgctgt ccggtctgat gatcggcatt
    17341 ggctgtgtgg tcagtatgtc ctgtgaaaac cgctatctgg gtgcattttt gttctccctg
    17401 gggctgttct gcattgtgca gtttcagtac ggtctgtaca ccggtaaggt aggatacatc
    17461 ttcgaccggg acaaggcata tctgggtgag acgttcttca cgttcctggc gaatgccatc
    17521 ggcgcaagcc ttaccgccgg catcgtgcat ctgacccggt tcgccgcaga tacaccggta
    17581 aacggtatgg atgtgacgct gatcgaccgt gcgcaggcga ccatgtctgc aaaactggat
    17641 gacagtctgc tgagttcctt tgtgctggca ttcttctgcg gcgttctgat gttctctgcg
    17701 gtagaaggct ctcgccggtg caacgagaag aagaacttca tcggcggcgt gttcatcgtc
    17761 gttatgccga tcatggtgtt catcctctct ggattcaacc actgcgtggc agatgtgttc
    17821 tattatttca tggctggctg cccgaatccg ctgcgagcgc tgtgctattt cccggcggca
    17881 attctgggca acctgtgcgg cggcgtgtgc attccgctgc tgaaacgcct ttcccggaat
    17941 ccgctgtaaa ggcaacaccg cacaaagccc gcctggcggc tttgcacgca atctgcttgc
    18001 tgattttccg tcatactgca cagaaatccc
//
LOCUS       DAWENO010000023        17864 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1317_length_17864_cov_12.982200, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000023 DAWENO010000000
VERSION     DAWENO010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17864
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1317_length_17864_cov_12.982200"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3914 a         5296 c         4634 g         4020 t
ORIGIN      
        1 atgcggagca gtatcagaag gacaaggaaa acgacgacac gctcctgccg ttcacctatg
       61 tgctttacga catggacaag gacggcacgc cggagctgat cgtccggacg ggctacagcg
      121 aggcagacta tgccctctac gtctggacaa cggacgcaga caagcagccg gtccaggtgg
      181 acggttcggg aaacggtgcg cacacccaga tctacggctc agaaaacgag atcggctttt
      241 tcatcaacag ctgtcacatg gacacggagt acatcgcaca gtactatctc accgagggca
      301 acgacttgaa agaagtccag gtctattccg gtgagatggc gaacgaggcg cagtccgcct
      361 acggttacta tgacctcacc gccggagacg ggttcttccg gctggacgag cagagcattg
      421 aggactaccg ctataaggaa agccccaagg taaaggatgc gaaatccgcc cagtcgctgg
      481 agggatacca aaaggaactg gcacagctga acgatgacaa tgcggtcggc taatcctata
      541 aagtaaaagc aacgccccgg caaacggaac ttttcaccgt ttgccggggc gtttgtattt
      601 atgatacgtt ccgcatcttt tccatggtga ttgccgtcac cacccaccgc agacagggca
      661 ggaacatgac aacctgcagg aagatgcgcc agggcttttt ccagcccaga cagggcgcac
      721 aggcgatgag ataccggttg ccgtaaacaa tgcccatgac gtagggaatt aacagggcaa
      781 tcaaatacag cagcaggggc agacacatca aaagtgtcac atagcccagc atcacagcac
      841 caaaaataat tgccggcagg aagataatgc cgaacagcag taccaacacc gcaaacatcc
      901 accagacact gagcagatac accagcaaaa ccacgccgga aaccagcatc agcaggaaaa
      961 acggcaggga gaaccagaga aaagtcagct gctgttttgc cgccagcttg ggggtactgc
     1021 cggttcggga aacggggacg caggtacaga tgcgcaccat ggtatagcac caggtcatga
     1081 cgcacagcag catccccacg ccaggcaccc actgcaccgg agacggcagc gtgcaaagaa
     1141 cacgaaagac cggattcata gctgcggcat cgtccagaaa tgccgccgga gaaagcattg
     1201 ccagcatcag cggcagcaat gcgcccgaca gagaaaacag atgcagcaag acctgggaac
     1261 gggtgcgttc cggatgcagt tttccggaaa catcgagaat caccagcacc gcacaggcaa
     1321 tgccgcagag aatgggcagc atgaggttac tcccgaatct gtccgctgcc ggtgaccaga
     1381 tatttcacgg tagtcagctc gtgcagaccc ataggacctc ttgcgtggag tttctgggtg
     1441 gaaatgccga tctctgcgcc gtagccgaat tcgccgccgt cggtgaaccg ggtggaggcg
     1501 ttcacataga ccacagccgc atccacagta cgctggaact tctgggcagc gtccaggtca
     1561 cgggtgatga tgcactcgga gtgtttggtg ctgtacttgg cgatatgcgc cattgcatcc
     1621 tccaggctgt ctgccacctt gacggtgatc ttatagtcgt tgaattccgt gtcgtacagt
     1681 tcttcggtca ccggctgaac cgcatcgccc agaatctccc gggtcttttc gtcgccgtaa
     1741 atggtcacat cgtgtgaacg gaacgccgcc tgcactctgg gcagaatctc tgcggcaacg
     1801 tccttatgga cgatcagtcc ctcgatggcg ttgcagacgg acgggcgctg ggtcttggca
     1861 ttgtcaatga tgcgcactgc catttcataa tccggctcgc cgtgcaggca gctgtccacg
     1921 tagacgtggc agtttccggc accagtctcg atgaccggca cggtggactg gcgcagcacc
     1981 gcctgaatca gaccggcgcc gccacgggga atcagcacat ccaggtatcc ggtaagatgc
     2041 atcatgtctg ccgctgtctc acgggaagta tcctctacca gctggatgat gtccctgggc
     2101 agtcccacgg tctccactgc atcccgcatg atctccgcca gtgcggtgtt ggaatggatc
     2161 gcctctttgc cgccacggag aatgacaaca tttccggctt tcagacaaag ggtcgccgca
     2221 tccacggtaa cattgggacg ggactcaaag ataatgccga taacgcccag cggcacacgg
     2281 gtcttgagga tttgcagacc gttgggacgg acagagccgc tgtccaccga accgatagga
     2341 tcttccgcag cgatcagctg acgtgccgca tctgccatac cggaaatacg ggcaggtgtc
     2401 agccgcaggc gatcctggag cgatgcggtc atgtgattct gcacagccgc ctccaggtct
     2461 ttggcattgg cggcgagaat ctcctcctgc cgggaaagca gggcatcggc aatggtggca
     2521 agggcgttgt ttttcacctg gggtgcggca gtggcaatgg cggattccac agcctttgcc
     2581 ttgacgccga gggtttctgc atagttcatg ggaatgctcc tttccgaatg ataaaaagtg
     2641 gaattgacga ttaaaagaag atttttttga ttcagcaatt tttctaaaaa aatgctggat
     2701 caaaaaactt tactgatatg aagaactttt tcactgcgta ttacaacaca tactgcaaaa
     2761 aacgtcttac tctgccataa aatgcgttcc caccggttcg ccgtcaaaca gcgcatacag
     2821 ctgttccggg tctctgccat tcatgatgac catgtccacg ccagcctctg tggcaatttt
     2881 tgcggcgttg atcttggtcg ccatgccgcc gctgcccagt gccgaacctg cgccgcctgc
     2941 catctgctcg atatgctcgt cgatcttgtg tacctcccgg atgacctgcg catcggcgtt
     3001 ggtgcggggg tcggcagagt acagcccgtt gatgtccgac agaatcacca gcagatctgc
     3061 gcccactgcc gccgcaaccg tggcagacag ggagtcgttt tcgccgatct ccagttccag
     3121 ctcgtcgatg gcaacggtat cattttcgtt gacaatggga ataacgccca tctccagcaa
     3181 ccgcccgatg gtgttctgca cgtggggcag gcgctcgttg gtcagcgtgg cacgggtcag
     3241 caggatctgc gccacagtca cgttatactt ggcgaacata tcatcataaa tgtgcatcag
     3301 ttcgcactgc cccactgctg ccgccgcctg tttggacgga gtgtccttgg ggcgctccga
     3361 cagattcagc ttgcccacac ccatgcccac agcgccggag gataccagca gcagttctct
     3421 gccttcgttg tgcagatcgg acaggatgcg caccagattg gtcatgcggc gaatgttcag
     3481 ctttcctgtt ttatgtgtca gcgtcgaagt ccccagcttg aggacaatgc gctttttctt
     3541 ggatacatct atcattttcg acttttctcc ttatggtgca gtgattacat tctgcggttt
     3601 gccgtccagc cagcagcgta cattttccac tgcctgctcc agcagacgct gccgggtctc
     3661 cagcggtgtc caggcgacgt gaggggtcag cgtgcagttt tccagatcag ccagcggcgt
     3721 gtctcccatc ggttcttcgg tcagcacgtc cagcgccgct ccggcgatca caccgtcccg
     3781 gagtgcctgc gccaggtctg tttccacgat cagtccgcca cgggcagtgt tcaccagcag
     3841 tgccgttggt ttcatggcag aaagccgccg ggcattcacc atgccggcag tctgttccgt
     3901 cagcggcacg tgcagcgtca gcacatccgc caggcgaaac gcctcgtcca ccgtcaccag
     3961 cggatacggg cagtctgccg gttgtgtccg ggtggcgatc accgggcgca tcccgaacgc
     4021 cgcaccgatg gcagccactt ttctgccgat actgccatag ccgacgactg ccagagtctt
     4081 tccggcgagt tcctgcatgg gatagggcat cacgcagaat gtctttgcct gtttccatgc
     4141 accctgccgc accgctgtgt catattccgc cacccggctg taatggtgga ggatcagggc
     4201 aaaggtatgc tgtgccaccg catcggtgga atagccgcct acgttgcaga cggtcacgcc
     4261 gtgcgccttc gccgcagcaa tatcaatgtt gttataccct gtggcgcact cgcccacata
     4321 gcgcaggttc ggacactggt caaacagctc cgccgggatg ggcgttttgt tgcacagcac
     4381 tgcatcggca tcgcccacac gctccgccag ctgttccggg gcggtgaggt catagaccac
     4441 cagttccccg aaccggcgaa agccgtccag agagacaccg ccgccggaca gcgtagcgcc
     4501 gtctgtgact accagtttca ttccgttttc tccgtgccgt cagcaggttc tgccgcaccg
     4561 tttgcatctt ccggcgtatc ctttgccgcc ttttttgcct tcacccgtgc agccacggac
     4621 aaaacaacag cacctaccag gatcaccagt tcaaacagac cgatggtgtt gaaccagacc
     4681 ttgttgtgcg gatcgccgac gtggatcagc agagatgcca ggatgccgat tgccagcagc
     4741 agctggtacg gccggcgcag ccggataaac tgcaccaaaa agaaaatgcc tgcgatcagg
     4801 gcaaaaacca gatgatacgt caatgaaatg gggaatgtac ttgcgtccat agtcaaaagc
     4861 tccttttctc ttcccggcat tttaccgggt atatacattt ttattatagc acagatgtgg
     4921 caagtttgca atgggttttg agactttttc cgcattccca cgcaaaagcg ccgccgacat
     4981 ctctgccggc ggcgcctggg gtcatttatt gcggtgtatt cagtgtttct tcgatcagga
     5041 cttgttctct tccgcaagcg ccacatcatc cggggtctgt gtttccggat tgctgtcata
     5101 gtccatcttc cgcacatggt actggacatc ttccaggatc ttgcggtttg ccgctacggt
     5161 gtctcccagg agtccgatgg tgatggtctg gaaaccgatc atcatcagca ctgccgccag
     5221 gatgagggac tgcacatggc cgccgcctgc gcccatgcag aagaacacca ggaaccggat
     5281 gcccactgcc agacccagca ggaacagcac tgtccccaga acgccgaaga agtgcatggg
     5341 gcgatacatc gccgtagaac ggaggatgac ggatgcagag cgtttcatat accgccacat
     5401 actggagaac agacgggacg gtctggtctc cgggttggtg cggatcggaa cggatgtcac
     5461 tgccatcttg cttttgcctg cctggataat ggtctccagg gtgtaggtgt actcgttgac
     5521 cacgttcatc cgcatggcag tctcacggga atacgcacgg aatccggacg gcgcatccgg
     5581 cacatccgtc ttggatgcca cacgcaccac ccagctgccc aggtgctgga acttcttctt
     5641 tttccaggag aagtgttctg tctcatcaat gggacgctcg ccgatcacca tatccgcctt
     5701 gcgttccagg atcgggcgta ccagcttttc gatatctgcg ccgcagtact ggttgtcggc
     5761 gtctgtgttg acgataatgt ctgcacccag ccgcagacag gcatccagtc cagccataaa
     5821 gccacgtgcc agccccttgt tccgacggaa cgataccaca tggtgaaagc ccagctcccg
     5881 tgccttctgc acggtgttgt ccttactgcc gtcgttgatg ataaggtatt cgatggtgtc
     5941 gatgccgtca atatgccggg gcaggtcgtt gtatgccagt tccagcgtct cctcttcgtt
     6001 ataacatggg atctgtataa tcagtttcat ttttctccgt tcctttctcc ttacgctgcc
     6061 gcaaattcca gatttttcag cgggattccc aaaagccgca catcagccgg gttgaccgtg
     6121 cttgccgacc acagctcgga ctgtaatgta atcacatttg caccctcgtt cagcagatct
     6181 cccgaaatgt ccagggagac cgactgaccg ttgttcgtat cgtcaatgac cattgtccca
     6241 gcgttttcgc cgtttaccag aacaatcacc ggataggtct cctgtccgat ctccttcagc
     6301 ggaatggtac agcccaggga tacaatcatc gtgtaatcct gtttttccag attgcagcgc
     6361 accgccgcat tggtggaata tgcccagcag aactcgccct ccagcgtata gaagccccgg
     6421 tattccgttc cggcgcagtc cgccgcagca taagaaagtt cctcctgcca gaactcatac
     6481 agggatacgg tccggctgct ctccgagtat tccagaggga acatcaccac ctggctgccc
     6541 cggttggtat agttttcatc cgtgctgcac tccgccgtgt ttgcataaac caggcggaac
     6601 tgctggtcga aggtgtcatt ccacgaaccg ctgcccagat agaacacatg gtcgtatttc
     6661 tcctggagct gttctgcctg ggtctgggga tccgccatca ccggatacgc atctgcgccg
     6721 gtcatggcac gcagcggcag gtacatggtc cacatatttt cgctgtccac gatcacgcag
     6781 tcgttttcgc cgatctccga tgccacatct tccagcaccg accactgcag acgggtgtcg
     6841 tccacgtggt tcatcaaaaa gcggtcatag ggcgacacga acagcagccc tgctgccatg
     6901 accggcagcg tcaccctggc acgcagccgg tcgatggtca ttgcgccgaa caccacagcg
     6961 atgggaagaa acggcaccag ataccgtccg taataatagt agtattccac atcgaaccgc
     7021 agaaatgccg aatagaacag gacgcagtag aatgccgcca ccagaaccac caggcgtttt
     7081 gcggattcca gataaaactt cggacggaca atgcccatgc cccacgccag cggcaggagc
     7141 agcagccctg tgccgcagat aaagccccag aaggcgctgc cctgaagttt cgccagcggc
     7201 tcttctgcag taacgcactt caggacaatg tagatcacca gcggcaccag caggagttcc
     7261 accaggagaa agcccaggcg ggagtcctgt acccggagca gatagttctc cggggtgatg
     7321 tcccggtatt ttcggtgtac caggcggtgc atcaggaaga ggtacagtcc gatcagcacc
     7381 agcgctccgg cgcagactgc cgggatcagt gtaggcagat ctgcctggga gatgcccagc
     7441 ccgaacagcg gactgtagtt gttcatcgtg tagaacggct ggacgtggcg catggcgaaa
     7501 aagctgatgc agtagatcac taccgaaagg ggcatgagaa tggcaaagct tttccggcgt
     7561 gtgagccaga acattccgcc gtaaagcacc acaaacaggg gcagaatcgt ataaatcgac
     7621 acgtggaagc acgaaaaaac aaccaccggc aaaatggaca tccactgccg ccggggatgt
     7681 tcttcatcgg tcaggaaata gaggaacagc accatcagca ccgacagaaa tccctcagtc
     7741 agcgcagact ttgccaccca gatcaccacc ggagatgccg ccgccgctgc acacgcaagg
     7801 gctgtgctgc tccggcgcag cttcagattc cggcagagga agaacaccag gaaaatggtg
     7861 cagccgtaaa atacggtctg cacatcctgc atctgtgcca tgccgaacag ctttccccac
     7921 atcgccagca gtgacgcata cgtggggatg ccgtgataga tgccggaaac cgggctgacg
     7981 ctcaggtcat acaccgtatc cgggtatgcc cggctgccga tgtcatagcc caccagatag
     8041 gaatgcacgc tgttgcggaa ggtctcctgc tgtgcctcgg aaagtgtatg gtattccggg
     8101 aaatcctgct ggcggtcggt gacgccgttc agaaagttga tggcaacggt ctgatagacg
     8161 ccctcgtcct gtcccatgcc gaacagctcg tgtttcatgg cgaccagcac aaatccgccg
     8221 gcgcacacca gaatgggcag ccagtaggga cggatgtccc aggaaaccgt catcagttcc
     8281 cgccagggtg tcttgtcccg ttttttccgc acgatcagtg ccgcagcgcc cactgccagt
     8341 tccagtacca ccgttccgac ggcacagggc agcagagaaa acacatccag cacaaaaaag
     8401 cccatggcgg tcacgaccca tgcgcagagc cagtagatca cgccgaacgc cagcatctcc
     8461 accacattgg tctttttcca ccaaatgcct atggtcaatg ctgccaggac aacgcccagc
     8521 agtactactc ctgtaaataa actcatccct ttttcaattt cctccttaaa aggcacacct
     8581 cgccgggcat cccggcagac aaaacctttc ttccagcgcc gccgtcagtt tttctccgca
     8641 ctgacccact cgatgatctg tgaaaagcgc tgcatgatac tctcccaatc ataattcgct
     8701 gtgatatacg cccttgcgtt ggaacacagc tgcatatact ctaccggatg ctgcatcaga
     8761 tagcgcacag caccctcaaa ctcaaaataa ttctgataaa acaaaccgcc gttgctcttt
     8821 ttgcagtgtc ccagcaaaac cgggctgccg ccgttcacca gcaccggcac ggacatcgcc
     8881 atggattcca gcagggtcat ggagacgccc atctgtctgg acgggatcac caccgccttt
     8941 gcgccggcga tggcgctgaa cttttcgttc tccggcaaaa agcccagcgc caggatgtcc
     9001 ggatgctccg ggatgcggca gatctttttt ccggcaagca ccagtttcag ccggctgttt
     9061 gtccgccgct ggaactccat gaaatagcgc atcagcatgg ggcattcttt cttttcgtcc
     9121 acctgtccca cataaagcag atattcgtcc tccagcccgt actgccgccg gaatgtaccg
     9181 gggtcggtct ggcgtggcac atccatcccc agccccatca cggcgcaggg aatgggttct
     9241 gtccgtggga accggcggcg caccagcagg cgctcctcct ccgacaggaa cacgaacgcc
     9301 ctgggtctct gatacaggct ttccagcgaa aagaactgca aaaacgacgt gttctgcgcc
     9361 atggggataa acaccgcccg gtttcctgcc agaggcatcc cggcataggt cagatagttg
     9421 gcatccgaca aaaacaggat cgccgcatag gtatcccggt aatgccgcag atagcggatg
     9481 cagtccggcg catagggtcc gtgcttttcg aaccactctt tctccgtcac cagattctgc
     9541 cgtccctggg caagctctgc caggtatgcc tcatcaaatg cctggaaatc cctggagcgc
     9601 atctgctgca ccgagaaccg gcgcaccgtg accccgtgga tggattccac gtcacggacg
     9661 taccagtcct tccaggtggc gctgtccact gccttggtgg tcagcacctc cacctcatac
     9721 cgattggcaa gatgctcggc gattttccgg gcataggtct cgctgccgcc gctgatctcc
     9781 agtccatacc ggggatgcac cagtgcgatt cgcttcatct tctcacgctc ccatcagctt
     9841 ctggaaaaat gtccggtact tttctacaat aatatcccac tcgaagttct cccggacgaa
     9901 tgcgccgccg tttgcgccca gttctgcggc tgcctccgga tgctccagga tatactccac
     9961 gcatccctcg aactcgaaat agtccttgaa gtacagtccg ccgttggaac gcttggcgaa
    10021 gtccttggtc acggcgcact gcgaagagac caggaccggt ctgccgcaga gccagctctc
    10081 catgatgacc agggaaaagc tttcgttgtg ggacggctgg cacagcaggg acgccgccgc
    10141 catggcattg tacttatcct gcttggagac aaagcccagg tcgtagacgt cgtccttcac
    10201 cgactccgga atggcgatat cgccgccgcc gatcagcacc agctgaaggt cgtcctgttt
    10261 ccggtgtttc cactcggcga aataccgcag cagggtgtgg acgtttttgc ccacgtcctt
    10321 tctgccggcg tacaggataa aaggcttgtc gatgccaaac tgtttccgga acgcatccgc
    10381 atccgactgg atcttggtgt ccatgccgat gcccatgcag atctgctctg tggtggtgaa
    10441 gtcatagacc cggttcgcca gttccatttc cggcatggcg ttatagatca tgccccgtgc
    10501 ctttacgtat gcctggcgga agaggcgcat atacacatat gcctcatcat ggaaacaggg
    10561 aatcagcaca gatttttccg ggcagacctg cattccgtaa taggtcgtgc cgaacatata
    10621 cggaataaag acatacagac cgtactcgtc ctgcgcatcc cgcatatact catacagctg
    10681 cggactgttg accatctctt ccacgaagat ctgctcttcc ttgggtgaga tctgcttgcc
    10741 gtccatcagc tttcggttca cccggtcgaa tgcctgggtg tcacgccggc gcaccgggaa
    10801 acggcggact gtaatatcct ctaccactgc cgtccctgcg gcgtagtagt tctcgttcca
    10861 gtctgcggtg aactccttga cacaggtggt aagtatctct acctccatcc ctgccttttg
    10921 caggtgtgct gtgacttccc gcagttccat ttctgcaccg ccggggatgt tttccccgaa
    10981 ccacggaatg acgaaaccta ttttctgcat gatgttgaaa cctcctgcca atgtgcctta
    11041 ccggcaacac ctttcttata aatcctttgt ttatcctctg tttttctgcc ggggatccgg
    11101 cactgccgtg tctccgctta cttccgtttc ttagaaaccg gcggaacggt atcgataaag
    11161 tgctgcaatt gtccctcaaa cactttacgg atctccggat acgcaaattc ctgctggcgt
    11221 ctgtgctgtt ctgcgatcac ctcacggcgc agctccgaat cccgcagcac ccggtctgcg
    11281 gcaagggctg cctccatggg atcgttggtg ggcagcagaa tgccgctgcc gcccagggtg
    11341 tccgaaatgg cggacgaatc gtatgccaga atgggcaggt tgaaatacat cgcctccacc
    11401 agcggcacac agaatccctc atgatcgctc atgcacagga acaggtctgc cagatggtaa
    11461 tacgccagaa tctgcgcaaa gggaatgtgt ccagtgaata ccacgttatc caattccagc
    11521 gcgcccacat agcggcgcag gcggtggtaa taccgctcca tgccgttata agaaccgacg
    11581 atgaacagcc gggacttggg attgcagtac ttcttatagc agtagaatgc ccggatgaca
    11641 tcctcctgct ttttgttcgg tgcaacacgt cccacaaaga taaagttggt atagccgtca
    11701 tccttgtagt gttccatgac cgatgcgtcc ggctctttgg cataatccgc aaagggaatc
    11761 agaatcggac ggacggtaat ggggcagcgg tagttcagtt cccgcaggat atccccgtta
    11821 taggcagagt ctgccataca gccgtccacc ttgtctgcca ggtggcagag tccacgcatc
    11881 ccgtattcgg cggattccat tgccgcatcg ttatagccct tgaaaaagcg tggcggtgtg
    11941 atgttatgga acaccatcag ctttcggcag gggtactttt ccagcgaaaa gttcagctcc
    12001 gagccggtgg acatatgata caggatcacg tcctcgccgg agagacgggg cattttttcc
    12061 acctgctgtg cagtgccttc cggcagtctg ccgtcaatga cttcggcata gatgccggtc
    12121 tgatagccca tgccacgaat gaccttttcc agcgcacgga catcgtttcc gatggcgtcg
    12181 cccatagcaa gcgtcggcaa aagctgcaat actttcatcg agcgccctcc tcacccagct
    12241 ttgcctggag cgcagcgatc tgtgcctgca tctgctccat ggcgatcttg gtattcttct
    12301 gctggagttc cagtgcctcc accttttcca gcagttcctt ggtgctgtgc atccgggatt
    12361 cccgaatgta cagttccacc tgctgttctg cctgtgcgat gttcgcattc aggctgctct
    12421 gttctgccgc atagggttcg acatagaagc tcatgaactt ccgcagcacc ttcttgaaaa
    12481 agacggcgac gggatttccg gaaagcggac gataggggtc tacccggtgg tgttcgctga
    12541 tatactgtac gtttccacgg agcatatcgg catcaaaccg ctccacatag atcccggcat
    12601 cggcatctgc cggcacatcg gcaaatgaca gcatatcact gctgtaaccc ttttcctgaa
    12661 tttctgcccg gataccggac atgatctctt ctacattgac actcattcgt gtgacactcc
    12721 tttacttttc cagcgaaacg ccggttacct tttcataaaa cttcatatcc aaaaagatgg
    12781 aattgatgcc ggtgaaggca tattccacat accccttttc ctgcatgaac cgcaggatct
    12841 cctggttttt ctgccctgcc accagctgct tggaatacgg gatcatttca ataatcatgg
    12901 cagtgggacg atatttgtcg aagtcgatgc tccgcaggat ctccatttcc atgccctcga
    12961 tatccaggtt caggatcttg ggcgcaccgc ccagctgttc catcagatcg tttactgcaa
    13021 cagtctgcat ggggatggtc tccagcaccc ggacagacgg attttcccgg agtacctccg
    13081 agacatcgcc cggcgcagac agaccgtcag cgctcagcac gtggaaatcc agtgtttccc
    13141 cggacaccgg tcccacgcag gcgttgatga cccggtcttc cggacgtgcc tgccgcagtt
    13201 cttccgccag tttcgggttg gcatccacca ggatacctct tgcgccctgc tggtagaaga
    13261 accaggtatt gctcatatca caggggtgat ttgcccccag atccagatac gtacactgtg
    13321 aaagcggtac acccagcatg acgaaaatgt acatgagaat ggcatcctcg cctgcctggg
    13381 aatagctttt cttgttgaac aggtgcagtt ccgacgccag cagccctgcc tcacgcagcg
    13441 cctcttccgc cttttccaga cggatccgtg tctgatctgc acttgcgtct gtctgccgca
    13501 aacgggcatc gatcgcattc cggtgggcat cctgtgcgtc agaatgcacc tccatatggt
    13561 cggtgcgctc ctgtaaggac aggatgcacg cctccatttt cctttctctt tctatcccct
    13621 gttccagctg ctgctccaac tgctgaatat gctgttccag cagatatgct ccggttgccc
    13681 ggtagatgca gcggcggaaa aaaccttttt tcacaatatc gctcatagct tttcttattc
    13741 tcctgtcttg cgctccagtt ctgttccgtc tacgaaccag cgggattcca ggcggcatac
    13801 gccgatgtcc cggtgttctg acgtgactgc aaacgcctgg atgcggcgga tgtcatcata
    13861 gtaggtgcca tccggtgcat gaactgcaac atccaggaaa taccgtcccg gcagcagatg
    13921 gtttcggaac cgcacctgga tctcgccgtc cttttgcagc gccacccagg aatcggattc
    13981 gatctccaca ttggtgccgt agcagtggac gctgtcctcc cggaaaatgc cgtagccaaa
    14041 gttgcccctg ggcgttttct ccggttctgc gcagtgatac ttcagccgca gggtaatctc
    14101 gtctccggtg cggaacacat actgtgccgt gccgtctgcc gccaacaggg cagcatccgt
    14161 gaactgtacc tgtcccgtcc ccaggcgcac tgcggatttt tcgcagaacg ccgggagttt
    14221 ctgccgggag agtgtctcct ggtcggcagc atcctctgac ttgccttccg gtttctgttc
    14281 cgagtcggtc tccttttccc gatgttcttt ctggtgttct gcctcctctc gtgccaggcg
    14341 gagctgttcc atctcggaga ggtagtgttc atccacatat ttcgggatgc cctcttcctt
    14401 gatgagaccg tcctcgatcc agatacagcg gtcgcagatc tgctcgatct gtcccatgga
    14461 gtgggagacg atcacgatgg tcgtgccgtc cgccttgatc tcctggagtt tctggaagca
    14521 cttcgcctgg aaattgacat cgcctacgcc caggatctca tcaatgagca gcacatccgc
    14581 atccacgtta atggcaacgg aaaaggcgag acgcatatac ataccggacg aataggttcg
    14641 caccggattg tcgataaacg gttccagttc ggagaagtcg atgatggttt gcagtcgtgc
    14701 atcgatctcc tttttgctca gcccgaagat agaggcattg gtatagatat tctctctgcc
    14761 gctcatatcc gggtgaaagc ctgcacccag ttcgatcaga ctggaaacac gtccctgaat
    14821 ggtgatcgtg ccgctgtcgg ggtacaggat acgggtcagc agtttcagcg tggtgctttt
    14881 tccgcagccg ttgtgtccga tcagacccac cgcctcgcca cgctttaccg tgaaggaaat
    14941 gtcccgcagc acctggcggt tttcatagcg ccggcgcttt cggaacagca gccgctcttt
    15001 gaaactctgc cccttgtcca aaaagacctt gaagtgtttc ttcacatggt ctactacaat
    15061 tgcattttcc tgtttgcttt ctgccgccat ttacaattcc tccgcaaatc cctttttcag
    15121 tcgtccgaac accagcattc ccagcaccag gaacaatgcg cccagcacga ctgcctccac
    15181 caggctggtc agatccggca cctgggaata gtacagcacg tcccggtagc agctgatgac
    15241 agaggacatg gggttgatgt gatagacgaa ccgcagtttc tccgggatca tatcgatgga
    15301 gtagaagatc ggtgtcatat acatccacac catggtgacg atgcccagaa tgtgttccag
    15361 gtcacggaaa tacacggtga ccgccgagga cagcatcgcc atgcccagcg ccaggaagta
    15421 ttccaccacc atgaccacag gcaggcacag cagcgccagc ggattcagcg aaatgccgga
    15481 gatcaccatg accgggaaaa tgatgaggaa acacagcagc atattgacaa agcagctggt
    15541 cacgtaggaa atggggatga cctcccgtgg gaagtagatc ttcttgatca gatccttctg
    15601 cgccacgatg gaactggagc cgccggtgat cgccgaggag aaaaagatcc acggaatcag
    15661 cgccacaaac agatacagat aatacttttc gatgccggct ttcatgatat aggtgaacac
    15721 gaacgtatac accaccagct gcatcagcgg attgatgaac gtccagagaa agcccagcac
    15781 agaacccttg tatctgccac gcaggtcctt tttcaccaga ctgaagatca tctggcggta
    15841 ctgatagatt tctttacaaa ttttcataga aacatcctgt ccggggtctg cctgaaaaca
    15901 gacccgattg aaagtaaggc tgcaccgcac aaagcctgtg cgtgagatga gccgttcgtt
    15961 ttttcggcag actgcacaga aattccgcag gcggctttct gcggtactgc cgaaattgtg
    16021 cagaatacac gtacatttta ttataaccga tttcgccgga aaagtcaatg tttttccgcc
    16081 acatccggca aaatcacggg agaatatgct gttttttccg aaaaatccag tactcgcacc
    16141 aaaaacaaaa accgaggcag atcaaatctg ccccggctct tccttttttg gcatctatat
    16201 cagatataac gctccgtatt tctgccgtac aaatcaattt tttcactacg ccgctcctga
    16261 aaaagcagca taaagcatca gagagaaaat ccctctgtca gctcacgctg acatctcagc
    16321 acaaggcatg ccctacgggt gccttttcaa gggagacttc ttcaaaaaag atctgtcttt
    16381 ggtgaaagct tacgctcttt cttcgatgta gtttacgatg tcgccaacgg tcttgatgtt
    16441 ttccagatct tcgtcagcaa cctttacacc gaattcttct tctacagaca taaccaggtc
    16501 taccaggtcc agagagtctg cgcccagatc gtcctgcatg gtagcttcca ttgttacttc
    16561 gtcctcttca acgcccagct gatctacgac cagttccttc agcttgtcga aaatttcttc
    16621 ttttgccatt ttttcttcct ccaaaaattg atgttgcacc tttccgtcgg aaaggcggcg
    16681 tcagagccaa aaagcaagct tttccgctct ccaaatcagc gcattctcag acttcctcga
    16741 acacgccaat tttcctaaac ttagtatacc gttctttttg caaaacgtca atatcttttt
    16801 gcaatttttc tgctatcgtt tttgacaaat attccttgat atttgccgag attttgtcta
    16861 tatcgccttg agcgcctgcc tcaggctccg gaatgatcgc ctctgccacg ccaaaccgca
    16921 ccatatcttc tgcagtgatc tgcatgactt cgcacgcctc ttgctctctg gacgcatcct
    16981 tccacagaat ggacgctgcg ccacggggcg agatcacgga gtagagcgca ttctccagca
    17041 ttgccaggtc gtcgcatacg cccagcgcca gtgcgccgcc gctgccagcc tcgcccagca
    17101 ccacggagat caccggggtt ttcagcatca tgaactctgc gatgttccgg gcgatcgcct
    17161 cgccctgtcc acgctcttcc gcctccacac cgcagaatgc gcccggggtg tcgatgagac
    17221 agatcacagg acggtggaac ttttctgcct ggtgtgccag gcggagtgcc ttgcggtagc
    17281 cctccggatg gggcatcgca aaattggacg ccttgttttc ccggagattt ctgcccttca
    17341 cctgtgccag aacggtgacc ggaatgccgt ccagtctgcc gatgccgccg atgatggcgc
    17401 cgtcgtcgcc gaagttccgg tcaccgtgca tctcatagaa atcggtaaag atccgtggaa
    17461 tatagtcccg gactgtggga cggttcttgt cccggatcag gagctggcgt tcccacgccg
    17521 tccggcgctg ctgctgttct tccattgtca cgcacctcct gcttcctgtt cccggtcata
    17581 gccgtggagc ttcagcagat gcgacagcgt actgcgcata ctgcggcgtt ccacgatcat
    17641 gtccacgaac cctttttcct gtaaaaattc tgccagctga aagttgtcgg gcaagcgctg
    17701 gcggatcgtg ccttcaatga cacgtctgcc ggcaaagccg atcagcactt tcggttccgc
    17761 cagaatgata tcccccagag acgcaaacga tgcggtcacg ccgccggtgg tggggtctgt
    17821 catgacccca ccaccggcgg cgtgaccgca tcgtttgcgt ctct
//
LOCUS       DAWENO010000024        17540 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1352_length_17540_cov_11.759051, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000024 DAWENO010000000
VERSION     DAWENO010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17540)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_17540_cov_11.759051"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4064 a         4726 c         4498 g         4252 t
ORIGIN      
        1 gctgtgcgtt gaagtcaact gcgtttgcag ccttcttgtt cagcagaaca gcgtcagtga
       61 tgtctacacg accgtccagg ttggtatcgc caagcagaac atcgcccgga gttacagaac
      121 cgctggttac gctgctggtt gcagttgtct cagtctcagt tgtgctgctc tcggtagtgt
      181 cgcttgtctc agtggtagaa gttgttgttg cagtaacggt tacagttact tcaacagtct
      241 cgccgtcctt agaaaccttg atggtggtct caccagcaga aacgccggtt acagtaccat
      301 tttcgtcaac cttagcgata gaatcatcag cggtctcgaa tgtgcagcct tccttattcg
      361 gcttcagagt ctcagtctcg cctacaccaa cggtgatgtc agacggagta accttcaggc
      421 tgtcagacgg atcctcagcg tcatcaaccg gcatatccgg atacagctca tacatagtac
      481 ccagagccat catgatgtcg ccagcatgcc agaatctgtg cagagtgaag tagtagttat
      541 cggtgttgcc atccttcttg tagtacttct cagcttcctg atacatcgga gacatcttgt
      601 agttggagcg gatgtctacg aatgctacgc cgtccttgat tacgtcaccg ttcggcatct
      661 taccagtgta acgatcagca gccagagtct tgccctcacc attctgcgta gaaccgttcg
      721 gcagagccag cttggtctcc cacagtctcg gcaggttggt attatggtca gaaacgccca
      781 gaccgatatc atcacgatac ttgttccact ctctgtcgag gagctgtttg cccaggtaca
      841 gggactgctg tgccagttct gtagcagatg cagcagatgc agtggtcttg ttctcatagg
      901 aagtctttgc agcagcgatg tcgtcagtct taacgccctt tgcctttgca tagtagatca
      961 aagtgtttgc cagagaagat acacaaccca ggtcgccgtc gccatagcca acgatggtgc
     1021 aggtcaggtt gttgttatcc tggtacttac cggtccatgt ctccggctga ccagaccaga
     1081 tcaaactgga cgggatggag aaggactcgc cgtcatcagt tacaccctgt gtcagatccg
     1141 gtacagtgaa ctcggtgtct gtggtgtcat acggttcata ctttgcagag aatgtcttgg
     1201 ttgcatttgc agtacccagg atggtgttgt ccatgaacca gcctacccag cgatccagaa
     1261 ccttttccag agcctcgctc agcttcagac cagtgtcgcc aaccatcggg tcatttttgg
     1321 tattgtcaga cttcacaaca tagtacagtt cagccagacg ctgtacagcc catacctggt
     1381 taccgatcca gtggttcgaa ccagggtcag catataccgg gtgttccaga taagccatgt
     1441 tgtagaatgt ggacacatca gacggatact tttcataacg tcccttccag gagttagtgc
     1501 agccgccggc gatcggacca tccttagaca tcagccacag gtacagttcc atctgtctgc
     1561 tcagagactt ctcgaagtcc tcagtagcgc cctgtgcctt catgcctgcg ttcagattgg
     1621 agtcatttac cagtgcataa gcagccagcg gattctggta gaattcgtgg cagtgagatg
     1681 caccgatctg ccatgcccag gagccgtcca ttgcgccgcc ccaggaggtg taccagttca
     1741 tcagataatg cttgccatta tcagatccgc cctgcttcca cttatcctgg cagccgattt
     1801 ccttgtagta cttatcgtac atattattac gggtttcatc acccatctta ccagccagtg
     1861 cggagataga ctgattaccg ccccacttgg agttaacagt ctgatcgcct acgccccatc
     1921 tgtttgcatc ataaacagcc tggattgcac ggtcttctgc gtccggtgcg ttggtataag
     1981 accactgttc agccggagta gcttcggtgt tgaagaagcc cttcataccg ccgttgcctc
     2041 tgataccata ggacttgtgc aggtcggtac aagcgtgcgg gatagtttcc cagcaggatt
     2101 cctgctcgcc gcgctggaag gtgttgatca gtgtgaactg accgccgccg cttgtagata
     2161 cagcgctctg tgcgccctga gagccgttta cagcaccaaa gccataccag tcatctacgt
     2221 ctgccagcca gtgcatcagg tacagtccct tatcactgcc gtactgagag cagaagctct
     2281 ggtggatcgg gttctgtgcg gtattgccct gcgccatatc ggtcgggtac atttcaatct
     2341 gttcccactc atcgctgtaa gtagcgctca gctgactgcc cttctgcatg aagttgccac
     2401 cctgctgatc cgggatcatg gttgcttcca tggtcttcca agccttagcc aggtcgccga
     2461 cagtctcagt agaagcgtcc tttacgttct ttacttcaac ttcaccgttc tttgcagcgt
     2521 ttacgatgtt atcacgcatt gctgcggtcc ataccaggta ggacagtgct tcagaagttg
     2581 tttcgtgacc atagtccgga gcctcgatac acagatcttc taccgagtga tacgggatgc
     2641 ccagaccgtc ggaaacggtg ttcgtgctgg acatatagcc gttctgtaca ccgttggtaa
     2701 caacgtcatc gtacagggac ataaagcgct gtgcatagga cttgtcgccg tatgcctcgt
     2761 ctttggttct ggtaccagcg gcagcagatg cagcggtcgg cagaactgcc gagcaagtca
     2821 ttgcagcagc cagaacagca gccagaaatg ttttgctttt cttgaattgc atagtttagg
     2881 aattcccctc tctgaaagat aaatgtagga aatgatgatt tccgctgtgt tcgcatcctt
     2941 tcttcacggc aggatccggc gggcacagca gcttatgttt ttgaacgcat cctttgtcca
     3001 ttttcacagg acgcacacaa ggatgccttc tttcatatca aaaattatac acccctttcg
     3061 aaagtatgtc aaccctttgc acaaattcca gctgtgaaat tttgtatttt tgtagctttg
     3121 cccagtttca cgctcgttgt tttgtgcaat atgacccaca tttttctgtc attgcgcact
     3181 ttttccgtct cattttgcat tttcgccgat tcaccgccgg attttcacag cactttcaca
     3241 caaatatgcc atactgcttt tgcgatactt acaagctttt ctgacggttg cagaatccgt
     3301 tttatccatt attcccatat ttcatgatac tgttacactt tatatatgac aagaacatat
     3361 tattttgcga taataaaatg agttgtaaaa agccgacaaa acggcttttg cagaaaggag
     3421 caatcatatg gttgaaattc aaaacctaac caaatattac ggcagcaagg ctgcgctcaa
     3481 ggggatcagc tttaccataa aggaaaatga ggtgctgggc tttctcggac cgaacggtgc
     3541 cggaaagtct actacattaa atattatggc tggtgtcatc ccttccaccg gcggaacggt
     3601 aaagatccag ggctatgaca ttgcacagca gcccgtcaag gcaaaaaagt gcatcggctt
     3661 tctgccggag atcccgccgg tatatccgga tatgaaggtc cgggagtatc tgcacttcgc
     3721 cgctggactg aaaggcatcc ctgccgccaa gcggaagggc gaagtcgagc gtgttatgga
     3781 acggctgaaa attacagacg tgcagaagcg tctgatccgc aacctgtcca aaggctacaa
     3841 gcagcgtgtg ggattcgcca tggcgatcct gggcgatccg ccggtgctga tcctggacga
     3901 gccgacggtc ggactcgacc ccacacagct gatggaggtc cgcaatctga tcctggacct
     3961 gcgccggaat catgccatca tcctgagttc acacattctg ggcgagatca gtgccgtctg
     4021 tgaccgcatc gtcatcatca accacggtga gatcaaggca gacgatacca ttgaacgcct
     4081 ggaagaggca gacaactccg gtctggtgct gaaactgaag attcagggca gctccagaga
     4141 agtgccgaag attgccaggg ccgtggaagg cgttgtccgt gtggacaaca tccagttcgt
     4201 acagaccggc atctacacct atgatataga gctggaaaac gacggtgtcc ggaatgcttt
     4261 gctctccgct ctgctccagc acggtctgga agtcctggaa gtctctgccg agaagaagaa
     4321 tctagaacag gtattcgtca agctggtcaa ccaaaagaca aagcactcca gtctggaaga
     4381 tctgctcagc gaaatggacg aagagactgc ccagaagagc ggcgatgctc cggcagacgg
     4441 cgcagacaca ccggcagccg atgcagacac agcagacagt acgcagaaag aggaggaata
     4501 accatccatg tttgcactat ataaaaagga attgcagagt tactttctgt ctcctatcgc
     4561 ctattttgtc attgcctttt tcatggcaac attttcgctg acctttatcc aggacatcgg
     4621 caatgtttca tcatctactt atacattctc gttcccgtcc atcttttacg tgaacatttt
     4681 ctatttcatc ttcctgttac cgctgctgac catgcggaca ttttcggaag aacggaaaaa
     4741 cggcacggag accctgctgc tctccagtcc gctgaatgtt acccagatcg tcatggcgaa
     4801 gtttcttgcc actgccacca tcctgctgat gatgctggtc gtctctctgt tctaccccat
     4861 cctcacctcc atctacggcc gtgtgatctg gtccagcctc atctgtacct acatcggatt
     4921 cttcctgttc ggtctggtct gcatcgcact gggcatcttc atctcgtctc tgacggaaat
     4981 gccgctgctg gcaatcctta tgagcgagct tgccatgctc ctgctgatgc tgatggacaa
     5041 tctgagtgta aactcgttcc tctccagcat tccggtgctg tccgatgtgc tgagctggtt
     5101 ctccaatcag acgaagtttt atgtattctc ccagggactg atgcgattct ccgatctgct
     5161 gggttatttc actgaaatcg tcgtattcct gatctggact attatttcca ttgaaaaaag
     5221 acgctggagc cggaggtaac ttgccatgag tcaaaacaac aaagaaaaac atttccacaa
     5281 ctggaaaaac actaaatttg ccggcatggc gtggattctg gcgatcctgg tcctggtcat
     5341 tgccgttgta ttgaatatga tcgtctcccg gctggatttc agctgggata tttccccgaa
     5401 caagcagtac agtctgagtt ccaccacaga aaagtatctg gatcagctgg acagcgaggg
     5461 aaagaccgta gacttctacg ttctgaccac ggaagagagt ctgaaaaacg atatggaatc
     5521 tctgacgctg taccgtgcgc tggaagaata tgatgcgcac aagtccatca acctcatctg
     5581 ggtcgatccg gacacagaca acgacaccat ggaaaagatc aattccgaca acgctttctc
     5641 cctctccacc ggtgacatga tctttatttg tgacaacgtg aaaaagcgtg ttccgggttc
     5701 ttccatgtac accacttata ccgatgacaa tggcaacacc accggtgagg acttcaaggg
     5761 tgaaaatctc atcaccggct cgatcaaggg cgtagcagaa aaccgtacac ctaccgttta
     5821 tttcctgtcc ggtcacggcg aaaaatccct ggacagctac actcgtttta cgactaacct
     5881 gaccaactac aactatgctg caaaagaact gaacctctct gaggcagatg ctgtgccgga
     5941 agatgcagcc ctggtgctgg tcgcagcacc caccaaggat attaccgacg cagaatccga
     6001 aaaactgaac gcctatctgg acaacggcgg caacatcagc ctgctcatgt cccccaacgg
     6061 cggcagcttt gtctacacca acctggaaaa gatcatgaag gactatggct tctacatgga
     6121 ctacgaccgg gtttatgaga caaacaacaa ctatcacatt tccggcgacg cctataccat
     6181 tcagtgcgaa ctgaacgaac tggaagacga cagcgaggcg accgacctga cctccgcact
     6241 ggtggatcag gggctgtaca cctttatgcc ggagtcccgg agcttccgct ataatgacga
     6301 aggcggcaaa tacaccatcg ctcccctcat cacctcttat gacagcgcag tcggcgaacc
     6361 ctacggcggc acaagcgatg acccggagga agaaagcggt cagctcctgc tggcggcaca
     6421 ctccaccagc gtgggcagaa acgactccaa gatggtggta ttcggaaacg ctgagtttat
     6481 cgacgatgaa cacgtcagcg acgataccgt tatcgtgccg gtatatctgt tcctcagcac
     6541 catcagctgg atgtacgaca gcgatatcaa catggacatc gcaaccaaat ccacgacaaa
     6601 cgactacatt tccctgcagg attccggatt tgcacagcgt ctgatggtag ttctcacggt
     6661 agtaccgatc gcaattatgg cagtgggtgt cggcgtgtgg atcaagcgcc gtaactcata
     6721 agcactgtct gaggaggcaa aacaatcatg aaaagacaac tcatcatcgg actggtgctg
     6781 gtactgctca tcggtgtgtg tttcggcaca tatttcggca tcgaccacta tcagacccaa
     6841 aaggaaaaga aggcggcaga agaggctgcg gcacttcagc tgagttcttt cacgtccgac
     6901 gatgtgacca agctggtgct gcatacgccg gaactggact acaccatcga caaggatgac
     6961 gacagcaagt ggcaggttgc cgagggcagc accctgcaca tcaacaccta ttatattgat
     7021 gcactctgca cttatggctg cggagtgact gccacaaagg atctgggcgc tgcggatgaa
     7081 aagcagctgg agacctatgg tctgtctgat ccggtttcca tcgcctacta cacagacgat
     7141 gcggacaaac cggtcaagac actctacgtg ggaaaacaga accccacaaa caccagtttc
     7201 tatatgatgc aggatgatga tgaccacgta tatctggtgg attctgacac tgccggctat
     7261 ctctatgtga ccaagacgca gctgcggtac cgttatctgc tggacgatct gagttcgtct
     7321 ttcactgcgc tgacgctgaa acgtggagat gaaacggtct acacatttca ggacatggac
     7381 ggcaactccg actggcagct cacagaaccg ctcagcacgc ctatcggggc agacaactcc
     7441 aagctgtcca acctcttcaa caacatcaca gagctggagg cagacgactt cggcgacagc
     7501 gatgtgacgg cagacaagta tgcctcctat ggctttgaca aaccggcata cacattccag
     7561 ctcacccagg caaacggtga cactaccacc ctactggtaa aggaatttga cccgtctgtt
     7621 tcttcctatg tggattgtct ccgggtggag agcaacgaga tcctcaagat ggactccaac
     7681 tacctcagct ttttggagga caatgcctcg gattatatgc tcagcaccgt atataaggtg
     7741 aatctgacag atatctctgc tttcaccgtg caatatcacg gttcgttcaa cgataccacc
     7801 ctggacatcg accgtagctt tacggcggac agcgacagcg agacctacag ctgtgacgga
     7861 aagcagatca caagtgcaga cacggaactg ctggaatcgt tccagacatt ctacactgcg
     7921 ctgtccaatc tgtcctatga gtcactggac actgccgcag atgttccgac tgacaccgac
     7981 ccggcgctgc atctgactta taccatgaaa gacggcagca cccataccat ggatctggtg
     8041 aagaaagacg acaccaccta ctgggcgttc atcgacggaa cattcaccca cgccgttgtg
     8101 cgccagcgtg cgctgagcgg cgaggacaag gtattggttg cctataccgc aatggaaaag
     8161 ctgctggacg ctgccgcagc ataaacgacc gcacaaatcc agaaagagga caccggctgt
     8221 gaccgatgcc ctcttttttc tgcgctgtaa caggcttttt tcatgaagaa ttcttaaaat
     8281 ccattgacaa aacggtaaaa atggattata attgtagagt gcatattgtt ttcatgatgc
     8341 aaggtctttc gaaaagacaa tgatatttta agcggctttg tgcagtgccg cttttccaac
     8401 agaggtgcag caatgagagt tcacaagaaa aagtcccgca gcaattcaaa aatctggatc
     8461 aatgtctcca tcggcgtttt atgcggattc attgttctgg tttttctgat cgttttactg
     8521 acacagaccg gaaagccgaa aatacagcag cagatccctg cggcagtcaa tgcccagaac
     8581 actgccgctc aggcagaaac agaaccggca acagaggctc ccacagaacc ggagactctg
     8641 ctggcatatc ccactgctgc ggagaatgtg accgaactga cggcaaacaa catctccagc
     8701 gaacacgctg ttctgctgga cgtggacaac aacaccattc tggcacagcg tgacccggat
     8761 gcacgcatct atccggcatc catgaccaag atcatgacac ttctggtggc agcggaacac
     8821 atcgaaaaca tggacgacac cttcaccatg acctatgaca tcattgcgcc gctgatcgat
     8881 gcagaggcat cccgtgccgg attcgaagag ggcgagaccg ttccgctaaa agatctgatg
     8941 tacggcattg cgctgccttc cggtgcagat gcgaccacgg cgatcgccat ctatctgtgt
     9001 ggctcggagg acgagtttgt caagctgatg aacgagaagg cagaagaact gggactggaa
     9061 aacacccact tcaccaacgc cagcggtctg tatgacccga atcattacag caccgccacc
     9121 gacattgcca tgctgatgag cgctgtgatg gaaaacgaca cctgccgtga gattctgggt
     9181 acctatcagt acaccacctc ggcaacagag cagcatccgg agggcattcc gctggaaagc
     9241 accatgttca gccggatgta cggcaacgaa gtgaccggca tcaccattga ggcaggcaag
     9301 acaggctata ccgatgaggc agggcattgt ctggtcagct atgccaccga tgcagacggc
     9361 aaacagttta ttgccgtgac ggcaaagggt cccacctatt ggagagcggt tttcgatacg
     9421 ttcgccatct acggtctggt acacgacggc tatccgatgc ccgacaacct ggcggcagag
     9481 gaaaccgacg aaaacggttc tgccattgat ccgacagaaa cttctgcggc tccggcggca
     9541 taaaccggaa cggtaatttt cacaggaaag gaggctggtt ccacgccatg agcaaccgga
     9601 aacagagaca cctcagcccc gccggctttc tccttgcgct ggcgctcctc atcgtaggaa
     9661 tatttgcagc agactatgtc taccgcctgt ttttccggag cagtacggaa aatatcatcg
     9721 gaaccggcgg cttttcaacc acatcttcct ccgccggaac tgcgtcagag gacggagaaa
     9781 atgcaacaga ttccacagaa gagacccagc ccagcggcat tgtgctgcag ctgacagcag
     9841 ccgaccaggc aagcggtccg ctggttctgg cggatgcgtc ccatccctat accggcactt
     9901 ctgtatggag cgatttttct gcggtaacag acgccaatgt caaaacacgc agcacctcgc
     9961 tgcaggttca gccggagatc atccagccca tctgtgagct gtttgatgcc tatgcggcgg
    10021 caaacggcta tgccaatctc cagatctaca gcacgttaga cggcacgctg gacagtacct
    10081 ccatctatac caatattctc cccgaccgca gtaccggtta cggattcgac atcggactca
    10141 tcaccagtac gggagaagtt gtcccctata tcaccaagtg caacgagtgg atggtggcaa
    10201 actcctggca gtacggcttt gtgctgcgct atcccagcga caagacagat accaccggaa
    10261 ctgcctatgc accgcaccat ttccggtatg tgggaaaaat ccacgctgcg atcatgcacg
    10321 agaacaactt cacgctggag gaatatctca attatctcca gaactatccg gtagagtcgg
    10381 gcggtctgtc ctattcggac ggcacacagt cctacagcat ctactacgtt cccgctgatg
    10441 ccagcggcac gaccaaagtt acgctgccag aaaacacagt ctatactata tccggtgaca
    10501 accgaggcgg ctttgttctc accattacca acggcgcagc atccggtgac accggggcag
    10561 cggagacgac agcggcagaa acgacagccg catattaaac tgcatccaaa gaaaatacaa
    10621 atcccccaaa gacggcgttt caccatcttc gggggatttt tgtagtttgt gtatttgctt
    10681 atcagttttt cagtacatcg ctccaggaga catccatgcc aacgccgtgc atcgcaatgt
    10741 acagcattag atagaacaga tcctgggtgt ccagcacgct gtcgttgttg atatccataa
    10801 gcaggatcag ccagcggttt acgttaggat tgtcattaaa gactacgtcc ttttcgccaa
    10861 ctgctcttct tgcaatgtac agcatcagct cgaacatatc ctgcgaatcc acacgcccgt
    10921 ccatattggc gtcgccacgc actctgggca gctgctgcgt ttgtttggtc gtggttgtcg
    10981 cagaagttgt ggtgactgtt gtcgtctgta ctgttgtagt tgtcgtggaa ctggaagtcg
    11041 ttgcagaggc agacgtggtg gtactggtct ctgtccacga aactgtcagc gttgtactgc
    11101 ctgtggtttc tgtggtgctg cttgtatttg tcgtcgtttc tgttgtatcc gttgttgtgg
    11161 tagttacggt agtgctggtt gtggtttccg aggtcgttgt ctccgttgtt gtcgtcactg
    11221 tggtagtcgt cgtcgtggtc gtcgtttcgg tcacagttgt ggtttctgtt gttgtcgtag
    11281 gtttggttac cttggtgtag ctttgtgtcg tagcgctagt ggttgtagta gtttccgtgg
    11341 ttgtcggtct ggtctgcttg gtatatgttc ctctggatgt cgttgtgaaa ggttctgtgg
    11401 tcgtggttgt ggtagtcgat gtggtcgttt ccgcagaagt ggatgttgtg gtagtggttt
    11461 ctgtcgttgt agtactggtc gttgcgggcg cagtcgtggt tgttgtggtc tctgcggtgg
    11521 tggtagtcgt tgtggtcgtc gtcgtcgttg tcacagtcgt tgttgtagta gtgaccggct
    11581 tttcctttgc ggtaatggta actgtaccaa gaaccgtttc gtaattttcg ctgtccgccg
    11641 ggacaaatcg ccattgcagg cggttttctc cggatttcaa ggtgtcggag gtatccgccg
    11701 cccaggtcag cacacccggc gtattggcgg aatccagacg aatcggcggc acagcatcac
    11761 cctctgtgta agaaacgctg tcatcccaca caacggagac cttgggtacg cccttttcga
    11821 tcacaaattc cagcgaattg ctgccgctcc aattggtatc cggcacaggt gcaatgcgaa
    11881 ttgttcccct gccggcgttt acgttgttct cataggaaag gagataatcc gttccctccc
    11941 tcaggagatg gtctcccacc ttcaccagaa cctttgtcgt cagttcctcg ccggtgtaat
    12001 agattctctc cgtatcagcc agagtgactt ctgccgcatt cagcggaatc gcctgcaatc
    12061 ccacagagag aactgccttg acattcgggt tgccggtgtc ataaacatag gtgatatcgt
    12121 tcagcggatc gtctgccaca acctttgcac cgtccacctg cgcattgctc cagtctgagg
    12181 cacgctccat gaggaaacgg ctccacagcg taggctccag gtacagctgc ctgccggaat
    12241 actgcggcgt tttcagcgca cggcagccgg tcagatcaca gctggtcatg ggtgcctctt
    12301 ctgagagata cagagacaaa agcggattgt gtccggcgtt cagcacattc agtttcttca
    12361 aagtggaaag tatcagcgca ctgatctggt ttccggaaca gtccagctct tccatgctgg
    12421 tctggtctgc aagttccaga gattccagct ggttatccgc acaggaaagc actgtcaagt
    12481 ttgtactgcc cagcgaaata cttgtcagtt catttttctg gcagtacagc ttttgcagca
    12541 ctgtgcagcg ccgtgtgtca agttttgtca gctggttgct gtcacagtag agttcttcca
    12601 gcgtttcatt ccgcagcgcc agtcccgaca gcgcattgtc gccgcagtcc agcagtttca
    12661 gtgcggacaa cgctgtcaca tccagctgtt ccaaatggag tccccggcag cgcagttccg
    12721 tcaagctggt aaaccgggaa acatccagtt ccgtcatggc gttcttgctg cagtccaggg
    12781 atgtcagcgt gtccggcagc ggagacagat ctgtgatccg attctcggca cagttcagct
    12841 ttttcaagtt ggagaagttt tcgatgccag tcagatctgc gataccgcag ccggaaatat
    12901 ccagctctgt gacgatctcc tgctccgact tctggagcat cccatcctga ttgcggtcca
    12961 gtttttccat gacatatacc cggaacgcat catccggaaa atgctcggcg tcaatcgcaa
    13021 tggaatcctc ttccattgcc tgcggcacaa gcgtaaaacg taccgttcgt tccagatcat
    13081 tggcgtcgat gcagtagtca tactgcacct ctgcggaaat atctgtcaca gtcagcagcg
    13141 tgccgtcaca cacggcattc tgccagctgt cctccgctgc aaactccggc gcaaagccat
    13201 ctgcaagcgt tgtcaaatcc agctgacaaa cagcattcac tggcacattg cgccggtttc
    13261 catctgccga cagtgtttgc agctgagaca gtcccgtcac atccagggcg atcagctggt
    13321 tctcaccgca ggaaagctct tccaacgaca gctgcttgga aacgtccagt gatgtcagtt
    13381 ggttccggct gcagtccaga cgggtcagct gccgtccgga aagatccagc gaagtcagct
    13441 ggtttccgtg gcagtccagt tcctgcaatg ccgacagtcc cgaaagcgac agttccggca
    13501 gctggttctc ggcgcagttc agactgttca acgtttcaca gccattgagg ttcagcgccg
    13561 tcagctgatt cttggaacag tccagggttt ccaggctgct gctgcgtggc aggaacaatg
    13621 ctgtcagtgc cgcattggca cagcgcagtg ttttcagctg ggacagtgaa atcacggaca
    13681 gacgtttcag cgctgggtta tcgccgcagg acaatgtttc caaagcggtg aagtactcca
    13741 cgccggtcat atctgcgatg ccctttccag aggcgtccat tgctgtgacc gccgcaatct
    13801 cctcagcgga aagcagttca tcctggttct ggtcgtactg acggacaaac gcccggaaca
    13861 cttcgtccgg gaaatgcgcc gcatcaatgg ggatacctgt ctccgtctcc tcagacgttt
    13921 tcagcagcag ggtgaatgtt accttctggt ccttgttcga tgtgggataa taggtgtatg
    13981 tgatgttctg ggacgggtca gcggcgtaga ctgcatcgcc gtctgtaaca acatttgtcc
    14041 agctgtctgc atccacacgg gagatctgga aatcgccggg cagatcagaa aggtaaaacc
    14101 gattcagcga atccagctgg acggtatagc gattccaggt ggcagacagc tttttcagcg
    14161 tcacctggtc ggaaacttga agtgaagtca gctggttgcc aatgcagagc agagtttcca
    14221 gtttttccaa tccggagacc ggcagcaccg tcagctggtt gctggaacag ttcagagaca
    14281 ccagttctgc acacttggaa acatccagct gcgtcagctt attgctggca caattcagcg
    14341 tattgattgc agaaaagccc gtgagatcca gcgctgtcag ctggttcata gaacagttca
    14401 gagataccag cttggtacag ccggtgatat tcagcacggt cagctgattc atggtacagt
    14461 tcagcgtttc cagttccggc gaaccggtaa gatccagttc cttgatccgg cactgtgcgc
    14521 agttcagtat ccgcagcgcc gggaagtcag agacctggaa ttcctggagc gtgctgttgt
    14581 aggaagagta cagttcttcc agccccttcg ccgccggcag tgtcagcttg gacagattat
    14641 tttgagaaca gtccagactt ttcagctgtg tcagacaggt gagatccagt tcccgcaggc
    14701 tgttctggga gcagtccagg ctttccagtt tggtgaaata gcggatgccg tccagtttcc
    14761 agatggaacg ctgtgaaatg tccatctgag tcacatcgtc cagttccgca cgggaaaaat
    14821 agccgtcccc gtctgcatcc agcgtctgta cgtaactgcg gaagtcgctg tccggaaaat
    14881 gggctgcgtc aatgggcagc aacgtctctt cggtcagcaa tgtaaatgtc acggcaatgg
    14941 cctttgccgg atcggtgtag tactgatacg tcacgtcttt gcccggttct gtcactgtga
    15001 gcgttgtccc ggaaagtgtg ccgttggtca gactgtccgg cacgatctgt tccgggacaa
    15061 atccgccggg cagggtcgac aggtcaaact gtcccaacac atctgcatca atggagacac
    15121 ggtttccgtc tgccagcagc tggtctgcgg tcagtttcgt ttccccggtg tccagagacg
    15181 aaagctggtt gttccggcag tccaggcttt ccaggctgtt ctgtccgctc agtgacagcg
    15241 ccgtcagttt gttctcccga cagctgagtt ttgccagcac ggatgctttc ggcagcacca
    15301 gttccgtcag accgcagccg ttacagctga gttcccgcag agcagacagc ccggagacat
    15361 ccaacgtttc cagcggcagc ccgtcacagt tcagcgtctc cagccagtcc agcttttcca
    15421 gtccgttcaa gtctgcaacg ccgctgtccg ccgggatcgt cagcactgta atttgttcca
    15481 gttcttccag agagaaccag ccgtttccgt cggtatccat gccgctcagc agactgcgca
    15541 gcgccgcatc cgggaagttc tcttcgctca gttccagata gcggtctgtc tccggctgca
    15601 aggtcaaagt gaaagtcgct accgggacag cctgcgcagc atccagctgg tagccatact
    15661 gcacctggga ttttccggct gcaatatgca gggtgtccgg gtcggtatcc ttgacttcgg
    15721 cgttttccca gccggtaaca cggctggtgt ccagctccgg cagctgggtc agatcatagg
    15781 tatacgcccc ggcacgctcc ggctgcggca caatggtgcg gctgtttccg gaagcgtcca
    15841 gcgaggtcag agaaagcccg gtgatgtcca gggctgtaag ctggttctct gcgcagttca
    15901 gcaccgtcag tgccggattc tcggtcacac tcagcgtggt cagctggttg ccggagcagt
    15961 ccagcgatgc aagcgaggtg tttttggaaa gatccagctg tgtgatctgg ttcttggcac
    16021 agttcagtcc agtcagcgca gtgttgctgc tcacggaaag tgccgtcagc tggttaccgg
    16081 aacagttcag tgtctccaag gcaggatttc cggtgagccg cagggatttc agcaggtttc
    16141 cggaacaatc caggatgacc agagactttt cccagatcag cgaaagggtg tccagccggt
    16201 tatcagcaca gttcagttct ttcagtccct ggcaataccg ggtgtccagt tctgtcagct
    16261 ggttgccgga acagtttact ttttccagaa gagaaaggtc gatcatggca agcgctgtca
    16321 gttcgttttg ggagcagtcc agttcgacaa gcgatgtatc tccgctgagt gacagggtgg
    16381 tcagctggtt atttgcacag ttcagcgttt ccagtacgct gtttcccgtt accgacagcg
    16441 tcgtcagctg gttgccggag cagtccagat cggtcagatt ctgatagtta aaacagctga
    16501 ggtcagtcag ctgattatcg gcgcagttca gttccacgag attgccgttt gcagcaacgt
    16561 ccaatgctgt cagcgcattc ccggaacaat ccagccggat gagcgtggtg tttccggaaa
    16621 gatccagtgt ttccagctgg ttgccggaac agttcagttc gcccagaaca atacagttgt
    16681 ccagcgaaag agaggacaag gcgcagtcgc tgcagtccaa aacggtaagc atagagccgg
    16741 gcatggagat tccgccaagc tgcatgggtt ccggcagcgt cagagattcc agcgtgccgt
    16801 tgccggagca gtccagtttt gccagcatcg cagcatcgga caagtccagt gttgtcagct
    16861 ggttatcgga gcagtccagg gcagtcagct tggtgaaatt cccgatgcct gtcagatcgg
    16921 caatttccat gccggacagc ggcaggtctg tcacgtctcc gcactctgtc tcggacaagg
    16981 cgccgtctcc gtctgtgtct gcattctcct ggagataggt tcgaaacgcc tcgtccggaa
    17041 aattctcctc ggtcagagca atctctccgg atgcggcggc cgtcccatcg gctgcaaaca
    17101 cagtcagcgg catacagccg accgccatca accctgccag cagaacactg agacagcggc
    17161 gcagtccttt atttcggctt cccatagcaa aaatcttcct ctctatttgt gcaagcttgt
    17221 tccgggcgga acaagctctg gttttttctg cctgacacag aacgttttgt gcagtttctg
    17281 tccagtttta tacatttata gtataacaca agcgccacaa aaagtcaaac aattttttcc
    17341 aattttggac ttgacgcagc agaatgcgtg ctgcgccgtt tccgagcaaa agccatgcgc
    17401 cacaacatga atcaaaactg ttttttcttg gaaggaatcg tgaattctac tgaaaaaccg
    17461 tcatttcaca caaaacggca ctgtcatttt ttacaaaaac gctattgaca agttggagaa
    17521 aatcgattat aatgtaagaa
//
LOCUS       DAWENO010000025        17390 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1371_length_17390_cov_12.588290, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000025 DAWENO010000000
VERSION     DAWENO010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17390)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1371_length_17390_cov_12.588290"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6082..6091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3799 a         4460 c         4706 g         4415 t
ORIGIN      
        1 cgtcgttttt catatctgca aactctaata tttcaaacca atcaaatttt tcgagtttgg
       61 acagggaaaa atcctctttg cggtagaatt ctccgttaaa atatccatag tgaaattgtt
      121 ctgaactgga aaatctctcg tttgttgcag ggtcaaagtt acaaattatg atatctccca
      181 gtcggtcaca tgactcagaa ccaagggtga tttttttccc gcataaaaaa gacctgggat
      241 tgtttttgat gtatttggat atatagtcag caacttcaca cattctcact aatgcacttt
      301 ctttttcttg accggaattt tcaaaataca tcttaatctc gttgctttcc tcatgataag
      361 acagagatgt gcattttatg cgttcatttt caacgcagat agtttgattt attttgtatt
      421 ctatatattt tgtttcatat ctgaaagcaa cattatcgta acaagatacg actaatgctg
      481 atgtgagaat caaacgaaca gccaaagcca gtgcaattag aaaatgtttt gtttttcgat
      541 tcatgtgttc acctttgaca gcggcatagg gacaacgatt gggtaaatgc tgatttttgt
      601 cgctcttagc cgaaaaatgc ttgtgtatac ttttttattg tacgatattt cgatggaaat
      661 tacaagcgtt ttgcggaaaa aagataggga gtggtttctg ttgctgtctt tgcaaattag
      721 gctcccctgt aggggagctg tcactgcaag tgactgaggg gttcttctgt gatatcaaga
      781 aaaagatttc atgaagaaac tcctccaccg ccttacggcg gtccccctcc tctttcagag
      841 gaggctcata cacaacaaaa agcggagaac gtcccaaaaa acgttctccg ctttctcttt
      901 tctccgcttg agacctgccg cttacagtgc ggcgatgacc tcaacctcta ccagcacgcc
      961 cttgggcagg tctgccacgc cgacgcagct tcttgccggc tttgcgtcgc ccattgcctc
     1021 ggcgtacact tcattgaatg cgccgaaatc ccgtgcaaca tccttgagga aacaggtggt
     1081 cttgacaacc ttgtcaaagc ttgtgcctgc tgcctccaga acggctttca gattcgccat
     1141 gacctgcttg gactgtccta cgatgtcagc cgcctctaca ttgccggtcg ccgggttaat
     1201 ggcgatctga ccggaagtgt acagcatatc gccgatcttt accgcctggg aatacggtcc
     1261 gattgcgtcc ggtgcgtttt tggtgtaaat ggtatccatg ataaaaacct cttttctttg
     1321 atataggtgt catgatgaaa atatttgctg gggtgtagag gagaatccct ccgtctgctc
     1381 cttacggagc atctacctcc cttttcaagg gaggcttatt accactcatt atgtccccct
     1441 tgaaaaggcg cccgtagggt gtgccttgtg ctgggatgtc ccgtagggac agggggatta
     1501 atccctcagt cagcctacgg ctgccagctc ccttttcaag ggagcctaat ctatgagatg
     1561 tgaaaaatta caatatcgta ggggcagatg cccacatctg cccgccgccg cacggctttt
     1621 ccggattctg cttagtccac cagatggaat cctgcgtcca tcagtgcgct gcggatctcc
     1681 tggatgtggg cgtagtctct cgtctccatg gtgatgcgca ggaaacagcc gttgatgtcg
     1741 ctgccctcat tggcacgctc gtggtggatg gagataacgt tgccgcccag gtctgcgatg
     1801 atccgggaaa cgtccttcag ctgacccggc ttgtccagca gctcaatgtt gagcatacag
     1861 cttctgccgg acatgagcag accacgcttg ataacacggg acaggatggt cacgtcgatg
     1921 ttgccgccgg aaaccaggca gactaccttt ttgccctcta ccgggacttt gtggaacatt
     1981 gccgcagcca cagatgccgc acctgcgccc tctgcgatga gtttctgttt ttcgatcagt
     2041 gccaggatgg ctgcggagat ctcgtcgtct gttacggtga cgatatcatc cacatacttc
     2101 ttgacatact caaaggtgtg gggacccggc tccttgacag cgatgccgtc ggcgatggtg
     2161 gaaacggaat ccagacaggt gatcttgtcg ttgtggatgg actcgtacat actgggtgcg
     2221 ccgctcgcct gtacgccgta aaccttgatg ttcgggttca gctgcttgat ggcgaatgcc
     2281 acgccggaga tcagtccgcc gccgccgatg gggacgataa ctacatcaac gtctgccatc
     2341 tgatccagga tctccagacc gatggtgccc tgtccggcaa tgacgttttc gttgtcgaag
     2401 gggtggacaa aggtgtagtt gtgttccttc tggagttcca cagccttgtt gtaggcgtcg
     2461 tcatagacac ccttgaccat gcagacctct gcgccgtagc tcttggtcgc ctctaccttg
     2521 gagatcggtg cgccgtccgg caggcagatg agcgctttca tgtggttctt tgccgcagcc
     2581 agcgctacgc cctgggcatg gttgccggca gagcaggcga taacgccacg agccttttct
     2641 tcgtccgtca gctgggacat catgtaatag gcgccacgca ccttaaagga accggtcacc
     2701 tggagatttt cggttttcag aaacagatcg cagtccggac acagtttcgg tgcctggatc
     2761 agatcggttt tccggatcac gctccgcaga acgtggcttg cttgatagat tttatccaat
     2821 gtcagcatgg ataggacttc ctttccataa taagattaag ggcaaagccg tcaggcgggc
     2881 tttgtgcggt attgccttaa gaatactgtg tcagcagttg gtcgatcagc tgttttgctg
     2941 cacgggcgct tctgccgctc ttctggagag cgaactgttc tgcctgtgcg tccagctctt
     3001 ctgtggtgat gtccagaccg ttctgttccg ccagagcgtg gacaatctcc agatacaggt
     3061 ttttctgcgg ccggctgtag gtgatggtga tgccgaaccg ctctgacaga gagatgcgct
     3121 cctgtacggt gtcgttggag tgcttgtcgc tgccgtcgtc gaaggtctct ttgaccaggt
     3181 ggcggcggtt gctggtggcg tagatgacgg tgttccgcat ccgtgccgca atgctgccct
     3241 ccagggtcgc tttcagcgtg ccgaagttgg ggtcgtcctc ggagaaagtc agatcgtcga
     3301 tgaagaggat gaacttcagc ggattccggc acaggtgatc catgatctgg gggatgtagc
     3361 acagctggtc cttgttcagt tcaataagcc gcagcccgtc gtccttgaac gcattggcaa
     3421 tcgccttgac ggtggcagat ttgcctgtgc cggcgtctcc ggcgaggaga acattggagg
     3481 catacttgcc ggccaggagc gccttggtgt tgtccaccac gatgctgcgc tcccgttcgt
     3541 atcccaccat ctgttccaga gaaatggggt cgggatactg caccggcacc agctcgccgc
     3601 ctgcgccgac ggtgaacata tgatgccgtg cgtagatgcc gtagccgtgc ttggcgatct
     3661 ctgccacacg ctgggtgtag agcgcatcca gatcggtctt atgggtctgc catgccggca
     3721 ggggaatgct ggtctgcatc tctgcctgga tcatggcagg ggtcagcatg gcaatgcggt
     3781 tcagcagttt cagttccgtg tgcaggcagg tctccaggtt cggggagatg ggttcgccgg
     3841 caccggcacg ctgcacatag acgttttcat gctccagaac gatctgttcc agatagccgg
     3901 tgaggtcatc tgcccgttca tacagggcag agaccatggc gctgtaaaag cgaatttgtg
     3961 ccggggtgtt ctccttatcg gtgtgacaga actgcatcag cgcccggatc accggatcct
     4021 gctgcaaact gcggaaaatg acaagggcat ccagcccgta tccgatttct tctctgggag
     4081 tcatatgggt tcatctcttt cgactatttt tataagtata ctgcttactt cagcagtgca
     4141 atgatctttt cggtgcattc ggtgcaggac agcggtgtta cgccctcgcc agccaggtcg
     4201 gccgtgcggt agcctgcgtt cagatatgcg tccacagcgt cctcgatcgc ctgtgcctcg
     4261 tccatgaggt cgaaggaata gcggagcatc atggctgcgg agaggatggt tgcgatgggg
     4321 ttcgccttgt tctgtccggc gatgtccggt gcagagccgt ggatcggttc gtacatacca
     4381 cgcttggttg cgcccagtga tgcggacggc agcataccga tggagccggt gatctgggat
     4441 gcctcgtcgg acaggatgtc gccgaacata ttggaggtaa cgactacgtc gaactgtctc
     4501 gggttcttga ccagctgcat tgcggcgttg tctaccagca tatcggtgag ggtcacatcc
     4561 gggtagtcct ttgccacttc gtgaaccact tcacgccaca ggcgggagct ttccagaacg
     4621 tttgccttat cgatgctgat gacgttcttg ttccgtttct gtgcggtctc gaaagccaca
     4681 cgtgcgatac gctctacttc catcttgctg tagcactcgg tgtcgtatgc ctcttcgccg
     4741 tacttgcctt cccgtctgcc acgttcgccg aagtagatgc cgccggtcag ctcacgcacc
     4801 atcatgatgt cgaagccctt ttccacgata tcttcccgga gcgggcagtc gcctttcagt
     4861 gccgggtaca gctttgccgg acgcaggttg gtgaacagac ccagcgcctc acggatgccc
     4921 agcagcgcct tttccgggcg gagatgcggc ggcatctgat cccacttgtt gtgacctact
     4981 gcaccgccca cggcgcccag caggacgctg tcgcttgcga gacagccgtc aatggtctct
     5041 ttcggcaggg gaatgccggt ggcatcaatg gcacagccgc cgatgaggta cggggtgcag
     5101 ttgaaggtgt gaccgtattt ctcgccgatc ttgtggagaa cagcaacagc gctgtctacg
     5161 atctccgggc cgataccgtc gccacggagt aatgcaatat tgaaattcat gggaaagact
     5221 cctttttgtt tgggaaacac tgaataaagc ttgtgcgtaa gatgcgtagt cagatttttc
     5281 ggcagattgc atagaaattt cgcaggcata cttgatgtat ggcaagggat ttctgggcaa
     5341 tatgacggaa aatctgcaag cagattgcgt gcaaagccgt caggcgggct ttatgcagtg
     5401 tttcctttgg tttggcagaa caaatcgtct ggatagcccc gtttgttccg caaatactct
     5461 ttcattatac accaagactg tgaagattac aaggtttttg tgaaaattct gcatttcgcc
     5521 acaaaaaagc cggtacgcag ggcgctgtgt cggtgatttt ggttgcggat tgactggaat
     5581 ggggttgtat ggtataatgg aaatgtggta aatcggaagt tgtggaggta attatgtaat
     5641 tatgaaagtg aataacagtt tatctgttac attgaaaaaa gtattattga ttatattcgg
     5701 tattttgcag ggaacgatag gaaattatct gggattattg ggatttgcat ttgcatttcc
     5761 gcaaactgat tcctattcaa aagactatga tgaagatatg ttccttgtcc catttgggta
     5821 tgtcatgatg cttacctggc ttattgtgac aatagcagcg gttatacttt tgcgaaaaaa
     5881 caaagcaaac ttattatcct atgtcattcc ttgggtaatt ggttttatcg gatgcttgat
     5941 cgtaattaag ttatggtaaa ttccagtttt ttcacatgag aagaacgcat agcgggcact
     6001 gtgtcggtga aatttgcgac ggattgactg cgttggaatt atgcggtata atggaaatgc
     6061 ggtaaatcgg aatttgtgag gnnnnnnnnn ntccttgtat atgtgcagat gttttttacg
     6121 gtactgaaat aatcagaccg gggggagaag cattgaattt tgctgcgcat gcctcgcact
     6181 ttaaggatat agatgttacg cttcttggtg ttgtcggaaa agataaatat gcagaagcga
     6241 taatggattc aatatcaaag cttgatattg ataaaagcca tatacgcatt gatgaaaggt
     6301 atcagactgc aaataatatg acttacctta cagaatcggg cgataggtat tataaagatg
     6361 attcatggaa cggagaaatt ctcgataaca tcgtactgaa tgataatgaa atcaaaatct
     6421 tatcaaggtc cgatgtagtc tttgttcatt tctgggcttc gtgtttttcg caagtagttg
     6481 aactgaagga aactcttggc tttaagcttg cggtagattt tgatgtatat agagattttg
     6541 cagatatgga acgatttgct ccgcatgttg atttctttat gataagcggc tcggaagaac
     6601 tcctgccgaa gttcaaagaa ttatcgaata aataccgttg cctgttcaac gtgtcacttg
     6661 cagaacatgg aagcgttaca tacttttatg gacaggaatt caaagtgcaa gctgtgaaag
     6721 ttgaaagcat aattgacacg accggttgtg gtgacagcta tcacgccgga tttgtctgct
     6781 catatatgct cgaaaataat attgaaaagg ctatgaatgt cggttctgaa attgcagcag
     6841 aaaccttaaa acattacggt ggattctgaa taatcagaaa cacaacttcc agttttcaga
     6901 caacaaaaaa gctgttgtac caaacaaatg gtacagcagc tttttgcata tcatagggtt
     6961 tcagagaacc gcttatttca cagcgccctt cttgatcgac tcgatcagac cgccgtttgc
     7021 aataatggtc tggataaatt ccgggaacgg tgccacgtgg aattccttgc cgttggtgac
     7081 gtcacggatg acgccgttgt ccaggtcagc ctctacggtg tcgccctcgc tgatggcgtc
     7141 cacagcctcc ggacactcca cgatcgcaag accgatgttg atggagttgc ggtagaagat
     7201 ccgggcaaag ctcttggcga tgaccagggc gatgccgctt tccttaatgg cgatgggagc
     7261 gtgttcccgg gaagaaccgc agccgaagtt ctcgccgccg accatgatgt cacccggctt
     7321 tacacggctg gtaaaggtgg tgtccaggtc ctccatgcag tgggaggcaa gctctttctt
     7381 gtcaatggtg ttcagataac gtgccgggat gataacgtcc gtgtccacgt tgtcgccgta
     7441 tttgatgaca gttccgttca gttgcatgat tacagtacct ccttcgggga aacgatcttg
     7501 ccggcaatgg cacttgctgc ggcaactgcc gggcttgcca gatatacttc agattccggg
     7561 tgacccattc tgcccacgaa gttccggttt gtggtagaaa ccgcacgctc gcctgccgcc
     7621 aggataccca tatgaccgcc cagacacggg ccgcaggtcg gggtggagac cacgcagcct
     7681 gcctcgatga agtccttcag cagaccgttt tccatggctt tcagatagat cgactgggtt
     7741 gccgggatga tgatggcacg gacgttcttt gccacctttt tgccacggat gatggaggct
     7801 gcctcttcga tgtccttgta gaaaccgttg gtacaggagc cgatgacaac ctggtcgatc
     7861 ttgacctcgc ccacctcgtc gatggtgcgg gtgttgcccg gcagatgcgg aaatgctacg
     7921 gtggacttga tctcggacag gtcgatctcg atgacctcgt cgtaaactgc gtcctcgtct
     7981 gccttgtaga caaccggctt gcggtcgctg tgttctgcca cgaatgccag cgtctggtcg
     8041 tccacctcga agatgccgtt ctttgcgccg gcctcgatcg ccatgttggt catggtgaga
     8101 cggtcgctca tggacaggga tgccacacct tcgccggtga attccatcga gcggtacagt
     8161 gcgccgtcta cgccgatctt gccgatgatg tgcaggatca cgtccttgcc ggagatgtac
     8221 gggttcagct tgcccttgag gacaaacttg atggcagacg gcaccttgaa ccatgccttg
     8281 ccgatcgcca tgccgcacgc catgtcggtc gagccgacac cggtagagaa tgcgcccagt
     8341 gcgccgtagg tgcaggtgtg ggagtctgcg ccgatgacaa cgtcaccgga aacgaccagt
     8401 cctttttccg gcagcagagc gtgttcgatg cccatttgtc ccacgtcgta gaagtgggtg
     8461 atctcctttt cgccgcagaa atcacggcac attttacact gggtagctgc cttgatgtcc
     8521 ttgttcggag caaagtgatc catgaccatg gtcaccttat ccttgtcgaa aacattgtcc
     8581 ttgttcagct tgttgaactc ccggatcgca accggggaag tgatatcgtt gcccagcaca
     8641 cgatccagat tgaccaggat cagctgaccg ggttcaacag aatccagacc ggcgtgtgcg
     8701 gcaaggatct tctgtgtcat tgtcataccc atggggtgag ttcctccttt atttgatttg
     8761 ccttagtcca tccggttgat gatcgcaccc ttatccgcag aggagaccat cttggaatat
     8821 cgtttcagat agccggtgag gttttccttg accaggatct tggtggtctt tttccgctct
     8881 gccagctcct cgtcagagac ctccagctgg atgctgtggt tcgggatgtc gatggagatg
     8941 atatcgccgt cctgtacata ggcgatggtg ccgccgttga cagcctccgg agaaacgtga
     9001 ccgatggctg cgccacgagt cgcaccggag aaacggccgt ctgtgatgag ggcaacgtcc
     9061 ttgtccagac ccatgcctgc gatggcagag gtcggggaca gcatctcacg catacccggg
     9121 ccgccggcag gtccttcata gcggatgaca accacatcgc ccttgacgat cttgccgccg
     9181 gtgatggctg cgatcgcctc ttcctcgctg ttgaatacct ttgccgggcc cttgtgtacc
     9241 agcatctcct tggctacggc gctgcgcttt accacgcagc gatccggtgc caggttgccc
     9301 ttgaggacag cgatgccgcc ggtctcactg tacggctggt cgatgggacg gatgatgtcg
     9361 gtgttcttgt tgatacatcc cttgatgttc tctgccatgg tcttgccggt gacggtcatt
     9421 acgtcggtgt ggagcaggtt cttcttggtc agctcgttca gcaccgcata gatgccgcca
     9481 gcctcgttca gatcctccat atatgcgtgg cctgccggag ccaggtggca gatgttcggg
     9541 gtgcgtgcgc tgatcttgtt gaccatatcc aggtcgatct tgatgccgca ttcgttggca
     9601 attgccggca ggtggagcat actgttggtg gagcagccca gcgccatgtc tgcggtcagt
     9661 gcattttcga aagcgtctgc ggtcatgatg tcacggggac ggatgttctt ccggtacata
     9721 tccataactg ccatgccagc ctgctttgcc agcttgatgc gctcggagta tactgccggg
     9781 atggtgccgt tgcccttgag acccatgccc aggacttcgg tcagacagtt catggagttt
     9841 gcggtgtaca taccggagca ggaaccgcag gtggggcagg cgttgttctc gaactcttct
     9901 acgtcctcca tggtgaattt gccggcagcg taagagccga cagcctcaaa catactggac
     9961 aggctggtct ttttgccctt gacatgaccg gcaagcatcg gaccgccgct gacgaatacc
    10021 gtcggcacgt tcactcttgc ggcagccatc agcagaccgg ggacgttctt gtcacagttc
    10081 gggatcatga ccagggcatc aaagtgatgc gccagcgcca tgcactcggt ggaatctgca
    10141 atcagatcac gggtcaccag ggaatacttc atgcccttgt gtcccatggc gatgccgtca
    10201 cagactgcga tcgccgggaa aaccaccggt gtaccgccag ccatggcaac gcccatctta
    10261 acggcatcga cgatcttgtc gatgttcatg tgtcccggta cgatctcgtt gtaggaggag
    10321 acaatgccga ccagcggacg ctccatctct tccttggtca ttcccagtgc gtggaacagg
    10381 gaacgctggg gtgctttgtc cacaccctca cagtaaaaat taccgctcat ttttttcacc
    10441 tcatataatt tcggaaaaat tgcttttccg tgtgtcacaa tggatctcag atacaaagca
    10501 ggcgcttatg tgtagccaaa aactgctttc ccgatattgt gtcaggaaag cagtttctgc
    10561 gctatagttt tatcctgcgt atcccatcaa tttagaaatg ggggattgct gtaaccagat
    10621 tagttgttga tcagcttgtc ttcgccgttc cagctgtaca gcttacggat ctcttcgccg
    10681 accttttcag acggatgctc agatgccagc ttacgcattg cacggaagtg tgcctggccg
    10741 cctgcctcgc tcatatccag caggaattcc tttgcgaaag aaccatcctg gatgttcttc
    10801 aggatctgct tcatggtctt cttggtctct tcggtgatca gcttcggacc ggtgatgtag
    10861 tcgccgtatt cagctgtgtt ggagatggag taacgcatac ctgcgaaacc gctctggtag
    10921 atcaggtcta cgatcagctt catctcgtgg atgcactcga agtaagcgtt tctcgggtca
    10981 tagccagcct cgcacagtgt ctcgaaacca gcctgcatca gagcgcagac accgccgcac
    11041 agaacagcct gttcaccgaa caggtcggtc tcagtctcgg tgcggaatgt ggtttccaga
    11101 acgccggctc ttgcgccgcc gattgccaga ccataagcca gtgccagatc ctgtgccttg
    11161 ccggtagcat cctgctgtac agcaaccagg cacggaacgc ccttgcctgc ctgatattcc
    11221 gaacgaacgg tgtgtcccgg tcccttcgga gcgatcatgg taacgtcaac atctgccggc
    11281 ggaatgatct gaccgaagtg gattgcgaaa ccgtgagcga acatcagcat attgcccggt
    11341 tccaggttcg gtgcaatgtc cttcttgtac attgctgcct gcttttcatc attgatcaga
    11401 atcatgatga tgtcagcctg ctttgcagcc tctgctgcgg tgaatacttc gaagccctgt
    11461 gcctcagcct ttgcccagga cttgctgccc ttgtacagac cgatgattac tctggcctta
    11521 tcgcccagag attccttggc attcagtgcg tgtgcgtgac cctggctgcc gtagccgata
    11581 acagcgatgg tcttgccata cagcagagac aggtcacaat cttcctgata aaataccttt
    11641 gcaacatgat ccattgtgaa agactccttt gaataaattt gggtttcaga aatgacattc
    11701 ctgtgcattt ctgacgcagt accgccggtt ttccgtgcag tctgctgatc catttatata
    11761 cggctgctca cgcatctttc aggcatgagc cgcctctttc aagagcgacc agaccggtgc
    11821 ggcacatttc catgatgcca tagggttcca gaagctcaat aaaagcgttg atcttagagg
    11881 actcgccacg gatttcgatg accagtgcct ccggggaata gtcggtgatc tttgcacgaa
    11941 agacatcgac tacggacatg atgtcctgcc gcttgtctgc tacattgttg atcttgatga
    12001 gcagcagttc acggatgacg gtctgatccc gtttcatgac cttcacggtc ttgacatcgt
    12061 acatcttttc cagctgcttg acgatctggt tgcagatgct gcggtcgcag tgggtggaga
    12121 tggtgatgcg ggagaattcc ggcgattcgg tctcgcccac agtcagtgta tcaatattga
    12181 atcctcgtct ggtgaacatc ccggaaacac gggacagcac accggaatgg tttgcaacga
    12241 ggacggaaat aacaaagcgt tcgattctat ccatgatggc acaccccttt acattgcaga
    12301 aatatcaacg atgatatcgt cgatgcagcc gcccggcggc agcatgggca gtacgaatgc
    12361 gtcgcagtcg atggcacaat ccaccaggac cggaccgttt tctgcgaatg ccttgggcag
    12421 gacttccttc agctccgcca tgttcatggc acgatagccc ggcataccga atgcctcagc
    12481 cagcttgacg aagtcggtct tgcgctccag ggtggtgttg gaatagtgct tgtcgaagaa
    12541 cagggtctgc cactggcgta ccatgcccag aacgccgttg ttcatgataa agacaacgac
    12601 cggggtctgg ttggtgactg cggtagccag ttcgttcaga ttcatgccaa agctgccgtc
    12661 gccggtgacc agaacggtct ttctgccggt ggcagttgca gcgccgatgg ctgcgcccag
    12721 accaaagccc atagttccaa gaccgccgct ggaaataaag gtgcggggtg tctcgaaggg
    12781 gtagtgctgt gccacccaca tctggtgctg tcccacgtct gttgccacga tagtgtcctt
    12841 gtctgccacg gcagcgatct ggtcgatggc ggtgaacggt gtcagctcgt ccggatcctg
    12901 tgcctccagc ttggcagtct ctgcctggag ttctgccaca cgagccatcc agtccgggtg
    12961 ggtctgctgg ggcagcttgt ccatcagacg ggtcaggatc tccttcatcg agccggcaat
    13021 gcccagctcc acgctgatgt tcttgcaaag ctcggatgcg tcgatgtcga tgtggatgat
    13081 cttggcgttc cgtgcatact ttgcggtgtt gccggtgcct ctgtcgctga agcgtgcgcc
    13141 ggcggtgatg atgagatctg cctcattctg tgcgatagag gaggcatagt gaccgtgcat
    13201 accctccatg cccagccagc ggggattgga ggtgggaacg gcagaaatgc ccatcatggt
    13261 gcagccgata ggtgcgtcga tcttttctgc cagtgccaga acctctgcgc cggcgttgtc
    13321 ggtgatcgca ccgccgccga tgtagatgta aggcttttcc gcagccagga tcatttccgc
    13381 agccttttcg atctcgctct cgtttgctgc cggaaccgga ttctttgcaa ccggtgcctg
    13441 tgcggtgtat tcatacagtg cgttctgtac atccttgggg atatccacca ggacaggtcc
    13501 gggtctgccg gactttgcga tacggaatgc ctcacggatg gtgtccgcca gatcttccac
    13561 acgctttacc agaaaattgt gcttggtgat gggcatggtc acgccggtga tgtccacttc
    13621 ctggaagctg tcccgtccga tcagagagtt cggcacgttg ccggtgatgg caaccagggg
    13681 aacggagtcc agatatgcgg tagcgatacc ggtgaccagg ttggtcgcac ccggaccgga
    13741 ggtggcgatg actacgccga cctcaccggt ggcacgtgca tagccgtccg cagcgtgtgc
    13801 tgcgccctgc tcgtgtgctg tgatgatgtg gtggatccgg tcctgtgcca gatacagggc
    13861 atcatagatg tcgatgacct ggccgcccgg ataaccgaat accgtagatg cgccctgttc
    13921 gatcagggtc tctacgatga tttgtgcgcc tttcaacttc atgttacttt caccttttat
    13981 ttttgaattt cagattatat aagatacaat agatacaggg agatacggga cagacccgaa
    14041 acgtatgccg cccggcacgc ttttcataaa gaaccgcacc gccgccgctc ctgtccggca
    14101 gctgtgccta caaaaaagcc ctcggctgca agtaccgaaa gcttccaatc ttcatgcaag
    14161 cgttgcggca gaataacagc cgttcgcact tttctttgga acaccggtgt gtgcagcaca
    14221 aattccgctt cgggtcaaaa tgactcgagc gcctagttcc acgataatca ctcgtgctgc
    14281 caaccggcac atagcgtcgc tgtacatcgc cgtacactcc ttttccaaaa aaatccctgc
    14341 gaaccctgcc gcatgacggt gcagatacac cgccgctgtg attcgtatgt gtttagtata
    14401 gcactatgtt ttcgcaattg caagtagctg tcttttattt ttacatttta gatacgtttt
    14461 gttcaataaa ttgacaggat acaaagaaaa aaccggcggt tttggtgaaa tcgaaagaaa
    14521 tttcgcaaaa ttgcttgaca aacaaagggg agaatgctat aatgaaaata atcacagtgc
    14581 cggagaacgg cgctggtgta tcatgaagaa aaaggctttg acaggaagat aagagaacgc
    14641 agactgtgtt tcagagagcc ggcggcaggt gtgagccggc agcagggcgt tcaggataac
    14701 tggtccttga gccgcaggcg gaacatacgg gcgctgtccc gtgtcagtag gctgtgccgg
    14761 acactccccg ttaccggagt acacattgtc agatgtgatg agcgggcgca tcagctgcgt
    14821 caaaaagagt ggtaccacgg agaatatgga tcttcgtctc ttttgtacag ataggttgtc
    14881 tgtatggaag gggctttttt tgtagacaga acagccgcag atcacacaaa caggaaagga
    14941 atgtatctta tgaaaaacgt agaacagtac gcaccgcagt tttttccggt aaagaatccg
    15001 cccatgcgct gggcatccaa gaccatgatc gaaaaagcac cggtctggtg cagcgttgac
    15061 ctgcgggacg gcaaccaggc acttatcaca cccatgaacc tggaagaaaa gctggagttt
    15121 ttccgttatc tggtgaagat cggctttaag gaaattgaga tcggctttcc ggctgcgtcg
    15181 gagacggagt atgagttctg ccggacgctg atcgagaacg atatgatccc ggacgatgta
    15241 accatccagg tgctgaccca ggcacgggaa cacattatcg caaagacctt tgaggcgctg
    15301 aagggcgcaa agcacgccat cgtacacctc tataattcca cctctgtggc acagcgggaa
    15361 caggtgttta agaagagcaa ggaagacatc atcaagctgg ctgagctggg cgcaaatctc
    15421 tgcaaggaat accgggcaaa gaccgagggt cacttccgct ttgagtattc gccggagagc
    15481 ttcaccggca cagagccgga atttgccctg gagatctgca atgccgtatt gaagatctgg
    15541 gagccgacac cggaagatcc ggtcatcatc aatctgccgg tcaccgtttc ccactccatg
    15601 cctcacgtgt atgcgaacca gatcgagtat atgaacgaca atctgtaccg gcgtgaaaac
    15661 gtggtcattt ccctgcatcc ccacaacgac cgtggctgcg ctgtggcaga cagtgagctg
    15721 ggactccttg ccggcggcga ccgtatcgag ggaacgctgt tcggcaacgg cgagcgcacc
    15781 ggtaatgtgg acatcatcac actggcgctg aatatgtact cccagggcgt cgatccggag
    15841 ctggacttct ccaatatgcc ggagatcgtg gagatgtacg agcgtgtcac cagaatgcac
    15901 gtgtatgagc gttcgccgta cagcggtcag ctggtattcg ctgcgttcag cggctcgcac
    15961 caggatgcca tcgccaaggg aatgcactgg agagaggaac acgactgcaa gcactggaac
    16021 gtgccgtatc tgcccatcaa tccggaagat gtgggacgtg tgtacgaggc agacgtaatc
    16081 cgcatcaaca gccagtccgg caaaggcggc gtcagctacc tcatggagac caagtatggc
    16141 tatcacattc cgcagaagat gcgggagtcc ttcggctatc tggtcaaggg tgtctccgac
    16201 cacgcacaca aggaactgat gccggaagag gtgctggaca ttttcaccaa gcactatgtc
    16261 aatgtggaaa caccggtctc cattccggaa gtccacttcg tacagaaggg cgaaaatgcc
    16321 atcggcacag aggtcaccat ctcctataag ggcaataccg agaccgtaca cggagacgga
    16381 aacggccgtc tggatgcggt ttccaacgcc ataaaggcac atctgaacat ggacttcgca
    16441 ctgaccacct ataccgaaca cgctctggaa gtgcgttcca gctccaaggc tgtggcatat
    16501 gtaggactgg aaaaggacgg aaagacctat tggggcgtgg gtatccactc ggatatcatc
    16561 aattcctcta tttccgcatt cctcagcgca ctgaatgcca tgatcgcaga tctgaagaag
    16621 taattctgat ttgcaataac gctgttttcc acatttgagt cattcactgt ggaaaacggt
    16681 acaatagaaa gagaaagccg ccgtgcagcg aatgttactg cacggtggct ttttgtattg
    16741 tctcagcgtt ttttagaagt aggtcgccat ggctaccagg aacatagcgt ccatgctgta
    16801 ccacttgccc ttgtactgga acacgatgaa gtcctcggac tcgttgtcct ttttctcatc
    16861 ggagcgatag cgtactttca gctcgtattt cacgcccttt tttacttcca gatcgctggc
    16921 atcctcaaag tcgaagtagt cggcgaagtc gttcttgtcc agcttttcct tgtcctcaaa
    16981 ggtgaccttt tgatgtcgtc gtattcgtca tagtatttgt ccagatagtc ctgcacatcg
    17041 ttggtcagtt ccttgcggct caaatcgttg tcatccatca gttctttcag ataatccttg
    17101 ggtacggttg ccatcagatc cttggcatcg caattctcaa tggctttgta atagtccttg
    17161 accacgctct tggagctgct gccgaaaaag gagcagagca ggacgatcac caggacggct
    17221 gtcaccacga tacagcctgc tgcaatgagc agtcccttgg tgttcatgcc caggaacggc
    17281 tttttgccgc ccggaacggc agtcactgca tctgcgccca tgccgtccgg ctgctggggc
    17341 tgttggggtg tttcgttttt cgtgccgcag ttcgggcaga actcgtcttg
//
LOCUS       DAWENO010000026        16948 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1416_length_16948_cov_12.683597, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000026 DAWENO010000000
VERSION     DAWENO010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16948)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16948)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16948
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_16948_cov_12.683597"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8707..8716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13136..13145
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4092 a         4084 c         4836 g         3916 t
ORIGIN      
        1 cttcttctgt ctactttatt gactatggtc cacttccttc gtggggcaaa acgccctcag
       61 acgaacagct tgacagcatc atgccgtgga gtgctactct gcctgaaact tgtcatagaa
      121 cgtatcatca gatcgttgaa aacgaggctg ttgttgagta acagcttctc attcacagta
      181 tttcatagta actggcagta tttcacgcac agtaattcac agcagttcac agtaattcat
      241 tgtgatgaac cgaatacagg atttgagccg aggttgatct cggcttattt ttttgcccgc
      301 tggtctggtt ggcggttacc ctgttatgcc ctccacgtta tactaaagac aaaccaagac
      361 aggaggcgta cagagatgaa aatattgatt acgacagatt gctatacgcc catgataaac
      421 ggcgtcgtca catcaattcg aaatctgaag acccagcttc gcaggcgggg acatgaggtg
      481 aagattctct gtccctcgca gtatctgcac ggagaaagcc gggacaatat tgtgcgaatc
      541 ggttccatcg gcgccggaaa attctgcaat ggtgccagag ccgctgtatg ttttcggaaa
      601 agcagtctgc aaaagctgat tgactggaaa ccggatgtca tccacgctca atcggagttt
      661 ttctcgttcg ccatggcgaa aagaattgca gctgcggtga attgccccat tgtacatacc
      721 tatcatacca tttatgaaga ttacactcac tatttttctc ccagtattgc actgggcaga
      781 aaagcagttg ttaccatgac tcggcacgtt ttacagtatg ccgaggcggt cattgcaccc
      841 agccgcaagg tggagacact tctgcggtct tacggggtgc agaaaccaat tgcagtgatt
      901 cccacagggt tggatttgca gcgctttgaa acccgctttc cacagccgca ggtggatacg
      961 ttaaaaagat ccctgggaat ttcgccggaa caaaaagttt tgctgactgt gggcagagtt
     1021 gccaaggaaa agaatctggc ggaactgctc gactatttcg ggcggctgtc tctgccggat
     1081 gcagtgcttt gcatcgttgg cggcggaacg tatctggaaa ctttgcagca gaaaagggaa
     1141 tcgccgaccg ggtgattttt accgatgcgg tttcaccgga caagattccg ttgtattacc
     1201 agatcggaga tgtattcctc agtgcatcgc aaagtgagac ccagggtttg acttatctgg
     1261 aggcgctggc aagcggattg ccgctggtat gccgaaagga tgcctgtctg gacggcattg
     1321 taacagatgg ctggaacggc ggtctgtata catcttttga atcgtttcag aagtggatta
     1381 cagcattctg cggcgatgcg gcagtaacac agcagctttc ccggcacgca gtccagaccg
     1441 cagagcgctt ttctgctgcc ggatttgcgg aaaaagtgga gttctgttac caggaagttt
     1501 tgaaaaattg gaaaatgcaa agtgcatgag aaggtggata tcaatgaaac cacgaacaca
     1561 tgatcgaatt gcaggaattg cagccgcaca ggctgatttt tctccgctgg agcgccattt
     1621 gttttgcctg ggcgcagcca ttccggacta tctgcccacg cagtttctgc accgccattt
     1681 ttaccagaag tcggcggact atgtctttcg gaaactacag aagcacagcg gcaggacttc
     1741 gctgtttgcc ctgtttcgtt tgggggaact gacacattat atgagcgatt tctgctgttc
     1801 tgtacaccgc agcggcggca tcggaaaccc acggctgcac ttgtcctatg agcgtcagat
     1861 gcagcggtat ttcatggaaa atctggactt gtttactgcc gagaaccgga tgatgcagat
     1921 tccgcagact gtcacagaag tgctgcaaac gtattaccgg cagccgtcca tggattttca
     1981 caccgacttg tcggcggcga ttcaggcgag tcttgttgtc tgcaaaaagt atgcgctttt
     2041 ggtgcagcag aaacaggcat tttcgggagg tgttccaaat ggtagctgtc attccggcat
     2101 atgaaccgga tgctgttttg acagatctgg tatagcgttt tttgattttc ccgacctcac
     2161 atcatcagaa ggaagtgtag accatgcaat ggatcacagc tctggatttt cagattctac
     2221 atgaaataca gaaccgcttt tcctgttctt ttctggattt ttggatgccg aaaatcacgg
     2281 cgctgggaaa cggcggtatt ctctggattc tccttgcagt actcctgctg tgcatttcga
     2341 aaaccagaaa aaacgggata cagctgggtg tcggcaatat gggatgcggt ctaatcggaa
     2401 atcttctgct gaaaaatctg gtggcacgtg accgaccctg ctggctggag gaacacgcta
     2461 tgctgattgc ggttccccag gactactcgt ttccgtccgg acataccatg gcatcctttg
     2521 ccgcagccac agtactctgg cactggaacc ggaaagccgg tcttgccacc tatcttctgg
     2581 caggcatgat tgcattttcc cggctgtatc tctttgtgca ttttccgtcc gatgtgctgg
     2641 caggcgcact gctcggctgt ctggtgggaa ttgcggcagt cagacttgta gatctgggcg
     2701 aaaagaaaat cgtgcataaa cgaaaagagg cgtgagcaat gcgaaaccgt tgtattttac
     2761 tgggcgtgga tattctgaca acatcctgtc tgcgtccgca taaatcggaa tacgttgttc
     2821 cggatgggat tctagttgtc cggtacgctg catttgttga taagaatgag aaaacagcat
     2881 gaactggatc tgtaaacaaa aacgctggat cagcatcggc tgctttctga ttacctgcgt
     2941 gtttgtagtg tttggctacc aaagcggtat cttcacagac acgcagaaga tgcaggcgtt
     3001 tctggaacgt gccggtgttc ttgcgccgct ggtgttcatg gcgatacagg cggtgcaggt
     3061 agtgatccca attctgcccg gtgcaatcgg ctgtgtgttc ggtgtcgtat tttttggcgc
     3121 agtcaagggt ttcttttata attacatcgg aatctgtatc ggctctgtgg ctgccttttt
     3181 actggcgaga gcctgcggaa aagatctggt gcagcaaatg accggagcga aattctatca
     3241 gaaatacagc aagtatttat tgcaggaaaa gcagtttgaa cgaatctttg cgcttttgat
     3301 tttcctgccg gtggcaccgg atgatttttt atgctatctg gcaggaatta gcaaaatgac
     3361 agtgcgcaaa tttgccatca ttattctatt gggaaagccg cttgccattc tgctctacag
     3421 catgggtgtt tatcagcttt tgcagagagc atgggcgctg cttggcagtt gaagtcctgg
     3481 aaaggaacaa atacgatgcg tgtcttttta tattccgagt tacagcgcca aattgaaaaa
     3541 agcggcgtgg gacgggcaat tcttcaccag cgaaaagcgc tggaggcaaa cggcgtctgc
     3601 tgtgccgagc gttggcagga tgccgatgtg gttcatatca acaccgtctt tccccattca
     3661 ttctttctgg cgaagaaagc gaaacgccat ggaatccctg tggtttatca cgcccattcc
     3721 accaaagagg actttcgcaa ttcctatgta gggtcaaact tgtttgcacc gctgtttcag
     3781 cggtggatca aattgtgcta ccagcagggg acgaaaatcg tgacgcccag cttttatgcc
     3841 cggagtctgc tgaaacagta tggcatcgag aaaaagattc acgtcatttc caacggcata
     3901 gccctttcgg aataccagaa ggatgcggcg gcaggacagc ggtttcggaa acaatacgga
     3961 tacacgccgc agtatcagtt tatctggttt ggctgtacca atttgcgttt cgttagcaga
     4021 aaagtgcgtc aggcggtttt gcatccgccg aagaatttgc agtttgccgg atacgtgccg
     4081 aaagagcagt tgcaggacgc ttacagcggc tgtgatctgt ttcttttccc gtcctatgag
     4141 gaaaccgagg gaatcgtagt gctggaggca ttggcaatgc aaatcccggt gctgctccgc
     4201 cggattccgg tttatacaga gtggctgaaa gaatcccagg acgtgtattt tgccggcgat
     4261 ttacaggaat tcgagacaaa agcggcacag atattacagg gaaaactgcc cgatttgacg
     4321 gaaaacggat accacgccgt gcagaaaaaa gacctgccga atgtgggaaa acagctgctg
     4381 cgggtatatc aaagctgtat ggaatagatc tgaccggaaa aaactatatg gagaaagcta
     4441 gagggagaac tctttttcgg gagttttccc tctgtttttt gtgcatcagc acaatcaaaa
     4501 ccccttttcg cacagttttt cgctttcttc acagaaaata tatcatattt ttgtgcgtat
     4561 cttttctgaa aaagggggct gatttggcgt atctgataaa tctgcacaaa acgattgaca
     4621 cacctgcacc gctgtgctat actagataca tcgaaagaaa acaaacagaa aagcaaagaa
     4681 aaaaagatgc tgaaaccgat aatgagacgg attcagcaga aaacacaaaa taatgtaaaa
     4741 cggcaatggg gctgttcaaa agttcccgtt gccgcttttt ttatttagga gatgagagtt
     4801 atgtcggaac tgcaaaacaa agttgaaatt cacatggaac atatcgggat ggtatatcct
     4861 gcggatggaa aggaagtcac tgcgctgaca gacgtgaccc tggatatcca gaaaggcgag
     4921 ttcgtctctc tggtgggacc gtccggatgc ggcaagacaa cgctcctgcg catcatcgca
     4981 gatctgctgc agcccacgtc cggtgtgatt acagtaggcg gcacatctcc ccgggaggca
     5041 agattaaagc ggcgctatgg catcgtgttc cagggcgcag tgctttatga ctggcgcact
     5101 gtgcagaaaa atgtggaaat gccgctggag atcatgaaag tgccgaaggc agaccgtgtg
     5161 aaacaggcaa agaaaatgct ggagcttgtg aatctgaccg agtttgcaga tcactatccc
     5221 aagcagcttt ccggtggtat gcagcagcgt gtgggaatcg ccagagcgct tgcaatccag
     5281 ccggagatcc tgctgatgga tgaaccgttt tcggcgctgg acgaattcac acgggaaaag
     5341 ctccacgatg acctgctggg catctggcga aagaccaata agacgatcat tttcgtaacc
     5401 cataacattg cagaggcagt ttatttgtcc gataagatct gtgttctctc gccgcatccc
     5461 ggcagacttt ccgctgttgt tccggtggac ctgccaagac cccgtaccaa ggcgatgaag
     5521 gacactatgg agttcaccga tctggtggca aaggtccaga acagctttga gggcgtatga
     5581 taaaggaagt gatatcatga aaaagattcg ccgcatttta gaggcaaaat ggctggttat
     5641 gctggtgtgg ctggcaggcg ttgccgtggt gtgggagata tttgcattcc gggtggaggc
     5701 ggtcaagcgg acaccggaaa acatcatgcc ccatatctgg cagatcgtcc aggcgatatt
     5761 cagcaccaat acggtgacca gagaccagac cgcactgcag gtcgttctga caagcgccgg
     5821 acagacgctg gaacgtgccg cactgggatt tgtcatcgga acgatcctgg gatttgtgct
     5881 ggcgctgctg atgcacctgt tccggggcgt ggagaaaact gtttcaccct atctgattat
     5941 cattcaggtc attccgattc tgggcatggc gcccattgtt ctggcaatca ccggcgatat
     6001 cggtgtttcc cgtatcatta tcgcagcgat cctgacattt tatccggtgg ctgcgaatac
     6061 gctttccgga ttccgggcaa ccgaacggga gaagaaggaa ctgatgtact cctatgcagc
     6121 caataagttt cagctgtata cgaaaaccta tattcccaac tgcattccct attttttcac
     6181 tggtctgaaa atttcagcgc cgatggcaat taccgcatcc atcctggtgg ataccctgca
     6241 gggtgacggc ggtctgggat gtatgctgtc gcaggcacag aaacacgcca tgtcgattta
     6301 cgtgttctgg ctgatcgttc tgttcagcgc ctgtgtgggc atcctcagct ttaccattat
     6361 gggctggctg gaaaatatgg cgtgtcctta tcgccgcacc ggaacccatc ggagaggagg
     6421 aaaacggcat ggcaggaaaa agtaaaaatc ggaaacggtg ggacgccgtc acaacagttg
     6481 cttatccgct gctgtttgcc gttttgctga tcgcggcgtg gcagaccagg cttttgcacc
     6541 ggctgctgca cgtagacacc tttattttgc ccattcccac gagaatcctg acgatcattc
     6601 aggaaaactg gggcgctatt gcggagaatt tgcagtccac cgtgatcgtt attgtgatcg
     6661 ggcttgccgc cggctcgctg ctggggtatc tgatcgcact ggtcgcagcc gcttttcccc
     6721 actggggcgc cggcggtctg accattgtgt cctgtttcaa cgcagtgcct gtggtagccc
     6781 ttgcgccggt catcatcaac tggacacgag atgtgagcaa tgacgtggca ttccgcagca
     6841 tggtggcaaa gtgtatcgtt gtcatgatct tttgtaccgc aaccatgagc atcaacgcat
     6901 tccggggctt gacggaagtg aaaccctttt cggaagatct gatgcgttct tatgcagcca
     6961 atcggtttac agttttctgg aaactgcggc tgcccaacag tgttccctat attttcactg
     7021 cgctgcacgt cagcgtgccg ctggcagtta tcaccgcagt ggtcagcgaa tattttgccg
     7081 agtatattat tggtgtcggt agaatgatcc gggagaacat tgtaaccgca cagtacgcca
     7141 ccgcatgggc atatattgtg atcgcctgtg cgctgggcat cgggatgtat ctgatctcga
     7201 ttttattgga acgtctgttc ctgcggaagc gcaaatgagc ggtttcgcag tgtgatagaa
     7261 aaaagcagga ttgggcgaat cgcccgcacc atgaaaggat gatggatgat gaaaaagcag
     7321 atgagagcaa ttgccgtttt ttgtgcagcc gtgttgatga ctgtttcagc gactgcctgc
     7381 ggcagcagca aggattcttc cggcagcggc ggcagtgcat cgaagggcag ctattttgac
     7441 aagggtactt cgtcggaaga tatcattaag gcagcctctg ccgccggaaa gatcggcaac
     7501 tggggcatcg gcaatgagta tgagctgctt gcactgctca ccaagtatga cctgcccaat
     7561 gagttcgtca gccaggcatt tgacatggac agctttgatg acgattctct gatgctggcg
     7621 tctgccatga cctataacga gctgggactg gtcaagaatg actatgacgg cggttatggc
     7681 tacggcgata cggtcggcac cattgatatg aacgacgagg gcgtagcgat gctggaggac
     7741 aacattttct gtacccaggc atttgctgag gaaaatccga acaccgttgc ggcatttatt
     7801 tccgcatcca tgaagggctg ggaatatgcc tgcgagaatc cggatgaggc ggcagagatc
     7861 gtgttcaact atggctcgtc tgtttcgccg gagcatcaga agtatatggc aagcgaagtg
     7921 gcaaagctgg tcaccacaga tatgtccggc aatgcagtcg gactggataa cctggggcag
     7981 atggaagaaa cagcaatgca gcagaccctg gatctggcaa agcagtacat caagctggat
     8041 gactctgcgg cggcagaaaa gctgggtacg ctgaccctgg atgatgtccg ggatacttcc
     8101 tactgggagg cagcaacttc tgaaagcttc ggcacaccag aaaagacaga tgtcaccgtt
     8161 cagctgaagt ggctgcccca ggcacagttc atgggctatt atgtggcact ggacaagggc
     8221 tattataatg atgttggtct gaacgtaacg atcacaccgg gcggcggcga tatttcagag
     8281 accaccgcag tatataacgg cacagtagac tttggcgtta cctggttctg taatctgatc
     8341 tctgcggatg cagccggcat ggggctgacg gaggtcgcac aggtttacca gcgttccggt
     8401 ctggaactgg tctataagct gaaccaggat tgagatggag gtgcagaaat atgcgtactt
     8461 tgatacagca cggtactgta gttaccgcaa gtgaaacgtt tttagcggat gttgcgatcg
     8521 acggcgaaac cattgtggag atcggaaagg acatctctcc ggagggcttt gacaaggtgg
     8581 tagatgccag cggaaagctg gtgctgccgg gtgcgattga tgcgcataca catctggcaa
     8641 tgccgttcgg cggaacggtt tcttccgacg gctattttgc cggcacaaga gccgcagcct
     8701 gcggcgnnnn nnnnnnccgt atttgacttt atcctccagg acttcgggga aacgatggtg
     8761 gatgccatca agcgccggga tgcgctctgc gcaccagagg ctgctgtgga ttattctttc
     8821 cacgtggcag tcaaggatgt gagcggcgag ctgctggaca ctatcgccga tgcggtggac
     8881 tacggcgtgc cttcgtttaa ggtgttcatg gtgtatgact tcggcgtgac agatggtgtg
     8941 ttctatcggg ttttgcagaa ggcaaaagaa gtgggcgcac tgatcgaggt acacgcagag
     9001 aacaacgaga tcatcaacac cctcacaaag gagtttctgg cagagggcaa gacggatgcc
     9061 tggtatcatt acgcatcccg tccggaattt gtggaagaag aggcagacaa gcgtgccatc
     9121 gcctgggcaa aagccaccgg cgcaccgctg tatatcgtcc atctggcgaa caaaggcggc
     9181 gtcgaggctg tgacccgggc aaaggacgag ggctatccca tctatgcgga gacctgtccg
     9241 cagtatctgg aatttaccag cgatgtctat aagctggaaa atgcacggaa tttcgtatgc
     9301 tcgccgccga tgaagggcga ggagagccgt caggcgctct gggcagccat tcgccggggc
     9361 gatatcgaca ccatcgccac agaccactgt ccgttccagt cctatgaaaa ggactggggc
     9421 aaggacgatt tcaccaagat ccccaatggc tgcgcaggca tcgagaatat gtatccctat
     9481 atgctgtctg cggcaaacag cggcgagatc acctttgagc aggcggtggc gctttgttcc
     9541 accaatccgg cacgaatctt cggctgtcag aaaaagggtg cgattgctgt aggcatggat
     9601 gcggatctgg tggtctatga tccggaaatg gatttcgtca tcaccaacga caagatgcac
     9661 tccgactgcg accacaccat ctgggaggga tgcaaactgc acggctatcc ggtgcagacc
     9721 tattccagag gcagactagt ctatgaaaac ggcgaatttc tgggcgaagc cggctggggc
     9781 aatttcgtaa agtgcaaatt aaaataaacg tgaaaccata gaacatatca aaaggaggaa
     9841 tgtgcgatgg cagagatgag ccggcggaca aagatcacac tggaacagca ggcggcacag
     9901 tgtatgctct gttatgatgc accttgtaca gctgcctgcg ggctgggtgc agaccctgcc
     9961 gcaatggtgc ggagtatccg ttttgaaaac ctttccgggg cagcggcggc aatgaaaaat
    10021 gcagcgtgtg catcctgtgc gcattcctgc aaaagcgcct gtccgcagaa gatgccgctt
    10081 gctgaaatgg cagcagcact tccggcggca gcggcacaaa aaccggcaga cctttccatt
    10141 tcgttctgcg gcgtgccgtg tgtcaatccc tttttccttt cctcctccgt tgtggcaagc
    10201 aattatgaaa tgtgtgccag ggcgctggag cagggctggg gcggtatcgt gttcaagacc
    10261 atcggctttt atcggccaaa agaggtctcg cccagattcg acaccgtgag ccgggaggga
    10321 tatccgtttc tgggattccg gaatctggaa cagatctccg accatacgct ggaggaaaac
    10381 ctggactttc tgcggcggct gaaacaggac tatcccgatc gtatcattgt tgcgtccatc
    10441 atgggcgaaa cggaggacga gtggacgcag ctggctcggc tggtgacaga tgccggatgc
    10501 gatatcatcg aatgcaattt ctcctgtccg cagatgaccg tagagggcat gggcagtgat
    10561 gtggggacaa atgtacaact ggtacaggca tatactgcgg cggtcaagcg cggcacaacg
    10621 ctgccggtgc tggcaaagat gactcccaat atcaccgata tgaccgttcc ggcagtcgcc
    10681 gctgtgaggg caggcgcaga cggtctggcg gcgatcaata ccatcaaaag cattaccggc
    10741 attgacgaag agaccatgca ggcacatccg ggcgtgatgg gaaaaaccgc agtcagcggc
    10801 tattccggca aggcggtgaa acccattgca ctgcgccata tttatgatat ggcggtctgt
    10861 ccggagctga acggcgttcc catcagcggc atgggcggca ttgagacctg gcgggatgcg
    10921 gtgcagtttc tggcgctggg gtgcagcaat atccagatca ccacagcggt gatgcagtat
    10981 ggctatcgga tcatcaccga cttgatcggc ggtctgacgg actatatgaa ccgtcacggg
    11041 atcgccagtc tccgggaact ggtaggtgcc ggactggagg ctgtgacagc accggaaaag
    11101 ctgaaccgca gttccatatg ctatccgaaa tttttgcggc aggattgttc cggctgcggc
    11161 agatgctata tttcctgtat ggacgcagga caccaggcga tccggttcga cactgaaaag
    11221 cgccggccgt ctctgatcgg caaaaagtgt gtaggctgtc tgctgtgtac gctggtctgt
    11281 cccgtgggtg cagtcacagc cgggacaagg cgttctacag aaacaccgga agagaggtga
    11341 ggtctcatgg cattaacgct ttcgcagctt tgcgtgaatg tggaaaagac gtatcagatg
    11401 aaactggttg ccggcgagga cggcatggac aatgtggtgc gctgggtgca tatcatcgaa
    11461 gatgtggaag tatcccattt tatcaacggg caggagctgg ttttcaccac cggcattgcc
    11521 cagtgcggcg tgaactggct gctgccctat gcggaacagc tgctgagcca cggtgcggtg
    11581 ggactggtca tcaatctggg accgtacatt gaaaaagtgc cgccgcagct gatcgtctgg
    11641 tgcaacagcc acgcctttcc gctgctggtg ctgccttggt cggtgcatat cattgatgtg
    11701 acatacgatt tttgccatcg catcatccag agtgaaaagc atgagcgcag cattgcatct
    11761 gcattcagca cctggatatt caatccggaa aagggcgaat cctataccgg gatactccag
    11821 cgggcaggct ttccggatga cggcagatac caggtcatgc tcctgtacct acgtgaaaag
    11881 gaacacgagt tcaccgatct ggagtgggag cagctgcagt tccgggtcta tcgggcgctg
    11941 cacaaaagcc acctttccgt ctgtattttc cggctggaac agagcatctg tctgatcggg
    12001 cagaatgtcc ggtcggagca gctgacacag tatgtggcgc aggtggcaca ggtcttgtca
    12061 gaacgaggtg atgtttactg cggcatttcg gaggagcaga tcggttacgt ctctctttcg
    12121 gcgctgtatc tccaggcaaa agccgccggt gcagtttgcc ggatccggaa aaaacaggtg
    12181 ctgggctatt ctgacctggg catctaccgc ctcctgttcg gcgtcaagga ccgcaggata
    12241 ctggaacagt tcatggaaag cgtgctgggc gcactgattc agtacgatgc cagaaatcac
    12301 accgattatc tggatgtgct gcggcgctat ctgctgacag agtgtagcat tcagcagacc
    12361 gcagagcaga tgcaggtcca ccgcaacacc atcaactata agctgcggca gatccgagag
    12421 attttgcaga cggatctgaa ccaggaggca agagtaaaaa tttatctggc ttatctcatt
    12481 cgggatatgc tgtaagcgta tcaccagatc aaatagaaaa attggaggaa ttcaaaatga
    12541 caggcgaaga gattcaaaaa atgcaaacca catataccat gcagtcctgg agcaagcaaa
    12601 agggactgaa acccattccg gtagagcggt cagagggaat ctatttctat gataccgatg
    12661 gaaagcggta tgcggatatg tcctcgcagc aggtcaacgt gaacctgggc tacggcaaca
    12721 aggcgatcgg cgacgccatc aaggcaaagg tggacaagta ctgctacatc gggccgtcct
    12781 tcggcgatga ggatcgtgca aagctgggca aggcgatcat cgacctgctg ccggatacgt
    12841 tcggcaaggt gttcttcacc aatgccggcg cagatgccaa tgaaaacgcc atcaagatcg
    12901 ccagaatgta caccggcaga aacaaggtgt tcagccgtta ccggagctat cacggttctt
    12961 cgttcggcgc cggaaatctg actggtgagc cgagacgcta tccgctggag ccgggcattc
    13021 cgggctttat caagtttttt gacccgtata tttaccggga accgctggat tttgcgtccg
    13081 aagaagaggc aaccagatat tatctggcaa agctgcggga acaggtcatc tatgannnnn
    13141 nnnnnagacc gcatcgctgc cattgaggtg gagaccatca ccggctctaa cggcattatc
    13201 attccgccga agggctatct gccgggcatc cggaagatct gcgacgagtt cggcattgtg
    13261 atgatctgcg atgaagtcat ggcaggcttc ggcagaaccg gaaagatgtt tgcctttgag
    13321 aacttcgatg tagtgccgga tatcattacc tttgcaaagg gcgtgacctg cggctatatc
    13381 cagctgggcg gcgttgctgt cagcaagaag atcgccgcat actttgacga ccactatctg
    13441 tcctgcggtc tgacctacag tgcgcatccg ctggcgtgtg ccgctggtgt tgcctgtgtc
    13501 aactactacc aggaggcaaa catcctggac aatgtggcag tatccggcaa ggtgctgggc
    13561 gagctgctgg aggatctgaa ggcaaagcac gcctgtgtgg gtgatgtgcg ctatatcggc
    13621 ttgttctccg gcgtagagct ggtaaaggac aagaccacca aagaacccct tgtgccgtat
    13681 ggaaaggatc cggacggcgt gatgggcggc atctgcaaaa agctgatggc aaagggattt
    13741 tccacctaca gccacgaaaa tgtcatcatt gttgcaccgc cgcttatcat tacgccggaa
    13801 cagctccggg aggagatgca gaagctggat gaggtgctgg gagaagtcga cgccgccatt
    13861 gccggctgaa aaaggagatg aggtcaatgg tatccggaca ttatgcgaat atcgaacaag
    13921 gaacgccgct gcggtcgctg ctaccggcgg accagtttcg caccgtctca caggagacac
    13981 gggataccgt tccgctgact cccaatgagg tggcggtgac ggtagttatg aaaccggcat
    14041 ttgtcaaacg gctgcacttt ccggtgccct gggacggaat cctgtacggc gtggtgcgaa
    14101 agccggagct gctccggcag ctgggaacag acgcagacgg gaagttctgg gtgaagtcga
    14161 tctcctgcaa cgactgggat gatcggtttc tgctggtgct ggaatcggag gatgcagaac
    14221 gctatcaggc tttttatgtg atggcggacg aggtgtatga ccttttggaa aattgtctgc
    14281 ggattccgga acagacaaat gaaaatgaga atccgaattt gtttatctga gataacatga
    14341 gaaaggaagc gcatgggtta tgtatgcttg cagtttggaa cgtatgaagg acaagatcgc
    14401 aacattttca aaattcggtg acgcaggtca cggcggcatc acacggtatt ctctgtctga
    14461 ggcagccatt caggcacgaa acgaattccg cagacgcatg gaggcgatcg gcgctgtgat
    14521 cgagatcgac gatgtggcga atatgtatgc cacacttccc ggcagcgacc ccgatgccaa
    14581 gcgcattgtt atggcatcgc acgtggactc ggtcaagaac ggcggcaact atgacggcat
    14641 cctgggtgtc atgtctgcca tggaggtgct ggagacagta cacgaaaagc agatcccgca
    14701 caagcatccg ctgactgcca tgatctggac caacgaagag ggttcgctct atccgccggc
    14761 aatgatgtgt tccggtattg tctgctatga ctatctgccg gaggacatcc gtgccaagtt
    14821 tgcctatgag gacatgatgc agtccaagag tatcctggac tgcaagagta ccttcggcga
    14881 ggcgttggcg aagtccggtt tccaggggga caggaagtac cgtctgaatc cggagcagta
    14941 tcagtatatg tttgaaaccc acatcgagca gggaccgatt ctggaggatg ccggaaatga
    15001 catcggcgtg gtggactgtg tgctgggaat gttcaactac cgcattcagt tctacggaca
    15061 gaccacccac gccggcacat tccccatgcc gaaacgaaag gatgcccttt atgcagcatc
    15121 cgaagcactg tgctatctgc accaggagat cgacaagctg ggttacagcg acctggtgta
    15181 caccactggt gagatcgtct gccatccctg tgtacatacc tgtgtgccgg atttcgtaga
    15241 cttttccttt gatgccagac acgaagatcc caaggtgctg gaacaggtcc tggcgatcgt
    15301 caagagctgt gctgacaagg aatggcacgg ctgcacctgc aaggtggaaa aggcgtggaa
    15361 ccgggatacc gtttactggg acaaaaagct ggtgggctat gtgaaggcag ccgcagagga
    15421 atgcggcatc tcgcatcagt atatccattc cggcgcaggt catgacgcac agtttgcatc
    15481 gtatatgctg cccacaacca tgatctttgt ccagtccaag gacggacttt cgcactgtga
    15541 gccggagtat tccaagccgg aacactgcac cgagggtgca acagtcatgc tgaacgcagt
    15601 gcttcgtgca gacgaggatt gaccatttca ttatcttgtg atatgatgaa agatatcaca
    15661 gaagaaaggc acagggaaaa taccatggtc aagaaattta cgacaacaca gaccattttt
    15721 accggatggg gcgcattgca ggagaatagt gcggcgcttt ccgggctggg gaagaaaccg
    15781 ctggtggtga cttctgccgg aacgtggaaa cgcatgggag cgcagttggc ggaacagctg
    15841 tccggatgct tttcggcata tgcggtgtgc gactgcatca acagcgaacc caccgatgtc
    15901 atgatcgccg aaggtctggc agcctatcag aaaaacggct gcgactgtct gattggcatt
    15961 ggcggcggca gtccgctgga tgccatgaag gccattgccg tcggtacgac cagttctgca
    16021 ccccttgcat cctacatggg aaaggagatc ggcggagcgc tcccgtctat ggcggcgatc
    16081 cccaccacag ccggaacagg ttctgaggtg acgaagttca ccattatcac agatacgaaa
    16141 acagatgtga aaatgctgct gaaaggcgag gtgcttttgc cgcagcttgc cattgtggat
    16201 gccagcttgt cggaaagtac gccgcagtca gttacggcgg ctaccggatt ggatgcgctg
    16261 actcatgcca tcgaggcgta cacctcaaag caggcacagc cgctgacaga tgcgctggcg
    16321 gtctccgcag taaaacgtat cttttcctat ctccctgcgg cataccgtga cgggaaaaac
    16381 cggaaggcac gggaagagat ggcggcagca gcgctggagg caggcatctg tatcaacaat
    16441 tccagtgtca ccattgttca cggcatgagc cggcccatcg gggcgctgtt tcatgtgccg
    16501 cacggcttgt ccaacgccat gctgctggaa aagtgcctgc agtttgcgct gccgggctgc
    16561 tatgagcggt ttggtgcgct gggacgtgcc attggcgcag cgccggcaga cagttccgat
    16621 gcggcggcag cagatgcgct gctggcggca gtgaagcagc tgtgccgcac ctgtgaagtg
    16681 ccttcgccgg aacagtacgg catctccagg acagcatttt tcgatgtcat cgaaaagatg
    16741 tcagcagatg ccatcgccag cggcagtccg ggaaatactc ggcgtgctgt aagcgcagcg
    16801 gactgcgccg ccatttaccg cagcttgtgg gaaacttaaa tcgacatctg cttaaaccga
    16861 catcttcgga tccatagaat ccataacctt ttgaactggt gtacaacatt gtttgtatgc
    16921 cagttctttt ttgcggcagg gaaaagtg
//
LOCUS       DAWENO010000027        16890 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1420_length_16890_cov_9.998931, whole genome shotgun sequence.
ACCESSION   DAWENO010000027 DAWENO010000000
VERSION     DAWENO010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16890)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16890)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16890
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_16890_cov_9.998931"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11458..11467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3960 a         4586 c         3895 g         4439 t
ORIGIN      
        1 accagaaaca ggcacatttg tacaatatcc ttttggtgcg gtttgatata gctttaggct
       61 atattgcttt gtgtgtacgt attcggatgc catacaaaaa ataacggagc aagatttttc
      121 acctgctccg ttgtttcatt tctgaaagac gtatcttcta ggacgcatct ttattgataa
      181 ctagatcctg gaatattttc agcaaatatc cgccaatgaa ggctgcgatg aaaaagataa
      241 tttccttgcc cacactgccg aaaacagcag cagcgatcat tacgtatatg ataccaatag
      301 gtatggatac gagttcatac agcgcttttc ttgccttctt ctttactgca aaatagatta
      361 gtttaatgat attcagcgga attgtccaga cagacaacag aatgagatag tcaatcacat
      421 gaattataaa agcaatttta ttatcagcaa gtttccatgc aaaggcaatt tcgctgtgat
      481 agtgaatgcc gataataatc agaatgttgt agaaaatcgc atcgtacacc aattcaattt
      541 ttccgtcagt cgttatttca gaccatttta tatcgtcatc atctttgtct gatttttccg
      601 aaatgactgc atcttctttg tcatcagcat ctggttttgc agtttcttcc ggctgatttt
      661 gttccggagc gttgttgctt tccacttttg ttccgcaatt tttacagaac ttcgtgccgt
      721 ctgcaagttc cgtgccgcaa ttcttacaaa acatcctctc gcccctttct gtgtcaatta
      781 cttcggatca ctgcgatcat cagtataaga aaaccaatca tatccagaac accgataccg
      841 attgcaatat aggctttctc acgttccggc tcgttgttgc gaatcgccag gtagccgaag
      901 taaatcgccg gcgcacctgc aatcagctgt acgccgcagc agcaccagca caaaagcccg
      961 aataccgccg ctttcaaagc agtgcttgcg taccctgttg ccttgctttc ctgaacgccg
     1021 gacgcattct gtgccggctg gacagtaact gcattctctt cctttgcaga actttcctgc
     1081 gatgcgccgc agtttggaca aaactggacg ccgtcactta tctgctttcc acattttttg
     1141 caaaacattc tctgcccttc tttacggttc taacgtgatg ccgtagtccg aaagatacag
     1201 cacgaatatt tcgttgtcat agatactgct cagcagatcg tcgaaatcat agtcatccag
     1261 cttgacatct tccttcacgc catccaccag gacatcattc agccagatgc cgtatatctg
     1321 cgacgaatcg tcatccatat ccttatcccg atagaactga atggtcacct tgttcgaaaa
     1381 tgtataggta tagctgtaag catcgccgtt ttgcattgtc aggtcggcat tgaactcaac
     1441 gacatccacg ctttcgccgt cgtcggtttc accgtcaaac tctttccagg acgcattgtc
     1501 aaagtagcca tagtttgcaa agacatttcc gatctttcta gtttcgctgt aataggacag
     1561 atggctttcc ttgacatcgt ctacagacag cgactttccg gaagaaaatg cgataattac
     1621 gacgactaca acgacaacaa gtaccaccac agcggctgcg ataatgccga tgatacagcc
     1681 ttttcccttt ttcttttttg ccggtgcggc tgcattcatc gcctgtgccg gatacggact
     1741 cgcctgcatg gtcggattga cgcctgcatt gctgtttgtg ccgggattgc cggcagcggc
     1801 agcatcctgc gccgcaccgc aatgctcaca aaatcttgta ccatcctcaa tttcttttcc
     1861 gcatttctga cagaacatat tgtttccccc tgattttaat tgtgattgtt cttgattgct
     1921 tctacttcac ttttccgttc ttcggcgctt acgtgatttc gaagatcacc aacggtgagc
     1981 gctgttcctg ctctttcaaa tactgcaccg gtatttaccg cattgctgat cttgaaattc
     2041 atagattcca caaagtcctg tttttcctgt ttcttctttt tcgagatctt tgacatcttt
     2101 atttttatct tctgattgtc attcagtgtc agtaaaaacc gtgcgttttt gaaaccaagg
     2161 aaaatcatga acaacaccag cacaatcaaa accaatatgc cagtcaagat ctccgtaccg
     2221 gtagcaagca gtgcaattgc caccaatgca caaatgagat aagtaaatat aagcatcggc
     2281 gaaatggatt ctttcagcga aaattctttt atcagataat acggaacgga agaaaccgtt
     2341 tctccatagt tatctgtttc atcaatcagc agacggttgt cctctacagt aaagtctgct
     2401 ttatatgcac cccggaactg ctttatcgta ttaaaatatc gagctgcgtc cttttgcatg
     2461 aatcttaaaa gaaaacgttt ttgctgcgtt ggaattcccg gctgctgctc cggaaatgtt
     2521 tcctgcggag ggtgttaagc gtgccccgca cttatcgcaa acctttgcac cctctttcaa
     2581 ttcattcccg caacttttac aaaacataac ttttcaatct ccttttcaaa aatttgataa
     2641 ccaaaataag ttatactgac attatactac aattttcttt ccgtgtcaat ccttttagag
     2701 attatttttt ataccctaaa catatttgaa tgcaatacga ataagcggta caatagaaat
     2761 aatactacct ttagggattg tcccatcttc ctctttgtac tgtctgcggc atccagtatg
     2821 aaacagatgc gtgaggaatg cggaacgctc ctgcataacg aaaggaacga tgctgctcta
     2881 tgaaagaaaa tcgagaaacc atatctggca taacaggcga gcgaatccgc aaattccgca
     2941 cagagcagca tcttagccag gaggagcttg ctttcgcatc cgaaatccac ccagcctata
     3001 tcggcaaggt ggaacgtggt gaaaaatgcc ccaccattga gacgctctac aagatcgcag
     3061 gcggcttgaa aattccgctg cataagctgc tggatgtcga ttccgacaca gcggcagaag
     3121 aatcggatgc ctttcaccgc atccagtctg ccatggacgg actgcatccg gatgagatgc
     3181 tgaaagtcgc caagatcgtg gaacaggtca ctgatctgat gcggcatcac tcctgatctt
     3241 ctcttcggaa tcctccagat tgcattcgaa gatctcgtac aggcgtatca aaagcagtgc
     3301 gttgctgaca ggaattttcc ggatgcgctc tgccttttcg tgcgctccga tcttgtccag
     3361 aaacaattcc agttcgccgt tgtaatagct gtagaccagt tcatcgatac agaaacatcg
     3421 ttttaagtcc tgaagagttt ccagatattc tccgttcatg tacatcgctg tcacctcctg
     3481 caatataaac taccacaatt ttttcttgac attcaattgt gatagcttat agatacgctg
     3541 ccgccgacag catcttctct ttcagcgttt ccgctgcatc ccggcacgcc tgtatctcgt
     3601 cctcgtccag ggtttcgtat gcctcgtcgc tgaggagatt gggaaaggac aggggcatct
     3661 gtgtctgaat ctttcgcccg atggtcatgg gaatgtcctc attgggttcg cagtacagat
     3721 aataccactc gctcagcgga gaaagattgg caaagcaaaa gtcgcagaaa tccatatccg
     3781 catcttccgc atacgctctt ctcagatggt cataaagctc cagctccgac tgatagcggt
     3841 tgaaattctt gccgttgtct ttcaagatct gatagtcctg gtactgcaat accgcattca
     3901 gattgggcat aaaatgcttt cgcttttgca tcatgcccgt ttttctcgca aggacggcaa
     3961 aaattacata cggtttcaaa gagcgcacct gcgcatcact gttgacatgg atcagcagcg
     4021 ctttcagatt ttcccggtag atctcgtttt tgatgccgtc gtgcagaaaa atatgctgta
     4081 tggtcagatg atacagaaaa tcggattcct ctgcatattc cgcctggttt ccctcgatgg
     4141 gctttttcag atgttccagc aggttctcat actttctcat gctttcatcc tgcacctgca
     4201 aaaatgcggt cagttcctgc atccgttccc gctgacggaa atagtttcgg acttcctgct
     4261 ctgcggagcg ctcctgcacc cctgcgttcc agagcagtcc gaaatccgtc acgatgcaca
     4321 aatacagata ctgcaaatcg gggctgccgg gatgctgttc catctgtgcc atcagttctt
     4381 cacaagcggc agcaaggtca cagtcactga acgcataccc ggcaagcagg ctgtgatact
     4441 tttcccggaa tgcctttatc tcctgctgtg cctcctccat tgtgcagtga ttctgtttca
     4501 gcagatcgtc aaattccaca tactgctccg cctgcgcatt gatatattgg tagattgcat
     4561 agtccatatc gtacatcatg aatttctctc cagtttcttt ctgattcgtt tgccctcttt
     4621 gctgttcaaa atgttcagat catccagatg cagcagaatc aacgctcgcc acagctcgtc
     4681 atcggattct tttgccagct caagggtcag ttttgtcatg cgcagttctt ctgcctcttc
     4741 cgtatctgca agtcgtttta tgatgcgctt gggaacaagt ttgtacattt cagtctcctg
     4801 tcttttttct cattatacac cattttcccc tgcatttcaa gtatcgggaa agaacgaaag
     4861 ccacccggaa acggatggct ttcacttatc atattgagat acagacttgc attactccgt
     4921 ttccgccggc ggattcggct cgatcaaggc aaactgacta tgcccgttca agtctgccac
     4981 agcatcgtgt atccgctgta gatactccgg atgctctgtc tctgtgaacg gcagcagctg
     5041 ctgcagatcc tttgccgtga ttgccgggaa gacagcgacg gcaatccgaa ccagcggcag
     5101 cggctcctcc gtttgtccct catagtagag aatcgttccg tgataaacgt gtaccgcaaa
     5161 ttctgcctcc actcggaaca cctcgcccgg atgccataca ccagatgcgc cgtccggctt
     5221 tgccgccagc tgtaaccgga atcggaaatc cgcatctgcc agtttttcgc cgtctgccac
     5281 aatgcccaga tagtccagca ccattctggg tgtcatgttc tggatcaagt cgtttttgcc
     5341 gtcctgttcc agccgccgca ccgttttgga agtgccgctg cgcagttcaa acgaaccgtt
     5401 cagataggca tttcgccaga tgccggattc tgactgatat gccagctgtt ccagggcatc
     5461 cgcattcagc agacgtgcct tctggtttgc cgggtctgca aacaccgcat aggaggctgc
     5521 ctttgcggca cggcgatagt ccccggcggc acagtcctgt tctgccaggt gcagtacctg
     5581 ttccacagag cccacatagt ccacgaacag ctttgcctct tctgtctcgg tcagcgcatt
     5641 cagttccgtg ggattgccgt tgtaaaagcc caggtatttg cagtagactg cccgggcgtt
     5701 gatctccaca gagccgtaat acggacgagt gtaccagacc ttcgccagtt tctccggcag
     5761 aaccacttcc cgtgcgatct ccttggcagt tctgccctgg ttcgccaggt gcagcgtccg
     5821 gtcgtggata tacttgtata ctgccgcatt gttccgcagg aaatcctcta cgctgtgcgg
     5881 tgtctccgga gtgttccagt gcggccagtt gtgagactgg aataccacgt ctgcctgttt
     5941 tccgtagcgt tccagtgcaa tcgccgtgaa ccgccaccaa ttcgccgcat cccgcagctg
     6001 tgcgcctcga atgggataga gattgtggag cgttccagtg cagttctccg ctgcccagaa
     6061 cgcccggtat tccggaaaat agttgttcat ctctgccggc gcctctgtgc cgggtgtcag
     6121 ctggaattcc acttccagcc cgtcaatggt cagcgtggta tcctccgcaa taaagtccgt
     6181 cggcgtgata tagctgagcg ttccggaaga agttcccagt cccagccctg tggagactct
     6241 gcccttcggt ccggcgagca gatcgccgcc gaactggtac ttggagcggt ggaacattgc
     6301 cgtcccggcg tagacatttt ccttgactgt ctcttcatcg aaaccagccg gcacgtaaat
     6361 gcgaacctgt cccggcgctt ttcccacctg ctgcggagac acaatgccct tgatgccgcc
     6421 gaagtgatcg gcgtgggaat ggctgatgat gaccgccttg atgttgtcgt gaatgttctc
     6481 gcccagcacc tcttccgtaa tgcgcagacc atacgccgcc gcctcgatgt tggtcgtcac
     6541 atcaatggcg atccagccgg tcttgctccg gacaaaggtc agattcgcca catcaatgcc
     6601 gcgcacctgg tagatctttc ccggcagcac ctcaaaaaca cctgcctcga tattcgcctt
     6661 gccgttgagc cacagactgg ggtgaaccgt atccgccacc gcctgctgtt tcagaaaatc
     6721 atacgcctgc aaatcccaga ccacattgcc ggtcttgtcc ttcagccgga atccgtccag
     6781 ctttttcagg cagccacgct gtgccagcgc cacttcgctg ttataggcat cttccaggtc
     6841 gatctgaaac tccctggcaa acgccgcatt tcgctgtttg gtgaatgcag ttgcctcttt
     6901 ggcagctgta ttataaatgt attcagacat tgcagtttcc tcctcttaaa tccgttccag
     6961 gaccggcggt tcccattcgc cccgaatggt ttgttcgtcc ggcgtataca cacgcagtgc
     7021 cagtgaaaaa tgttcttccg ttttcggcgc aggcagccag tttttccgtg ccagttcctc
     7081 ttccggttcg tcagcgaaaa tgaaaatatc cagcgaaccg tccggattct tttccacgct
     7141 gccgtcctgc agatcatagg tgttgatctt aaaccgtcca atgggattgg gcaccagata
     7201 cttgctgggt tcgccgtaca gcgtgatcga ccagaacacg gatgcgtggg gatagccgtc
     7261 tgccggaatg tgcagccggt agcggttcct gctttccagt ggtgcgccgc tgtcatctgc
     7321 cgccgcaaca acataggcgc tgtcctggat gatgttagcg ccccagccgc cataggcagt
     7381 ctcggcacgc tggagatagt ctgtgccata cgttccaacg ccgccgatca cggagagcca
     7441 gccacgatgc tgcaccgtat actgcggatt tcgctttccc ttttgaatct ccgcaaagcc
     7501 cagacggctg ccctctgtca gcgcctgctg ctgttctgcc gtcagcgcat cccaggaaaa
     7561 cgtaccggtc acacggtcat agccgaactg ttcaaaaatc tgtacatatg cctcgttccg
     7621 aatgggattt tccaccgaaa gcgccgcaaa cagggtgaaa aattccttgg gcgaaacggt
     7681 ttcgatataa gccgccggag aaatttccgg tgcctgccgc accgggcgca cccgctccgg
     7741 atacaggggt cggatggtaa tggaatcctg gatgcggttc actaccggat agtctgcctt
     7801 gccccgtgtc tcgatccgca gcagcactgc attcagagaa tcctgcaagc gaattacccg
     7861 ataggattca tagcctgccg gaaccggcgc atcctggtac agcaggatat aatcgccctc
     7921 tgccctgtcc ggcgtgcgtg taccgatgga ttccaccacg ttggtatacg catccagaat
     7981 ggggaacaga taatagcgct ccgtgatttt cggaatatgc accagatacg gtgtctgcgc
     8041 cagctgtgtc catgcggtgg agtacagcgt atcattgttc agttttacga tcttgttcac
     8101 cttgtcggtg ggaaatgtcc gcaggtggtg aaagcccttg tcgtccgaac cccagtgggt
     8161 cgcctccgtt accaccagcg gaaatccata gagatagagt gcctttgcct gttctttcag
     8221 gtttgccatg atatgttcct ccttattcac ttgttttcag tttttcataa taagatgtat
     8281 ccacaaagct gtccacgtca attgccttgg agatatagcc gttttccagc ataaagtcgc
     8341 tgagatagtt cagcttgtca atgacaccgc tgtcaatgtc gctgtccaga aaggcaaagg
     8401 tatcatcaaa tccatacgct ttccggaaca cgtccacacc cagtgtcggg gatgcaatgg
     8461 cattgtagaa ttcgtcggga ttggaaacag tctcctcctt ggcacgctgc aacgctttca
     8521 gcactgccac agcggcatcc gggttctcct gcaaaaagtc gtttctgccg gtgacggtcc
     8581 agagtcctgc catttccgga tgtgtaactg tgttttccac gtcaacgccc agtccctggt
     8641 tctcgtaata taccacatta taatacaggg tcagccatgc gtccgcctca ccagtggtga
     8701 aaaccgatgc cgcatcagag accacgttga cgatttccac cttgctttcg tcaatgtcat
     8761 aggcatccac caggtgtttc cagtactcct ggaagttggt gccgatgccg acgataactt
     8821 tcttgccttc caggtctttc ggtgtctgga tgccggagtc cttttgtgcc agcacaccga
     8881 tgttcatttg cttgtcggcg acgccgacca gacggatatc tacgccgttg ctgtaagccg
     8941 ttgccgccgg atagtctccg tagaacgcca tgtccagttc accggaggca tatgcctcgt
     9001 tgatcgccgg gcctgcctcc tggaatgtca ccaagtccag cgtgtaaccc acggcgttga
     9061 gttcttcttc cagatagccc ttgtcatagg caagcttgcc gttttccaaa agcggatagt
     9121 cgtcaaatcc cggactgccc agccgcaaag ttttcagatc accggaaaca gaggacgatg
     9181 cctgtttggt gctgctgtca gaagaaccgg cactgccgca gcccacgatg ccaaggctca
     9241 ggacacctgc cagaagaaat gcgaaaatct tattgtgttt catttgaaat acctccattt
     9301 actttgtgtt tcctttattc cagggcgtga tcgctgtaaa aatcgcaccc agaattttat
     9361 ccatcaagat accgattacg ccaatgacga tcatgcacac gatcaccaca tccgactgca
     9421 tcagactccg tgcattgctc atgcgatagc cgatgccgga cagggatgcg atcagctctg
     9481 cggcaaccac tgccatccaa gaaatgccca gccccagttt cagccctacc agaatctgcg
     9541 gcagtgcatg aggcagatag acctttcgga aagttttgaa atagttcagc ttatacagct
     9601 gcgccacctc gatatagcct gccggcgtac tggagatgcc gctcatggtg ttcatcataa
     9661 tggggaagaa tgccgccata acgatcacca ccaccttgga caattctccg atgccgcacc
     9721 agagaataat cagcggaatc catgcaatga tagggatctg ccggatcacc gtcaccgttg
     9781 gctgcagcag attggctatc atctttgaca ttcccatcag agaccccaga accgttccca
     9841 atgcgacgga gagcaggtag cctttcagca cacgggaaag cgtgaccgaa aggtcgttcc
     9901 agagttgacc gctttgcagc atttccagaa acgcctgcca caccatggag attctcggca
     9961 ggatgctggt cgggatgctg ccgaagtggg tggcacaaat ccacgccgcc acgatcactg
    10021 ccggaacaat cagccccagc aaaacctgtt tgattttctg cgttttcatg ttgtcctctc
    10081 ctttagatca tgtactctgc ctcttgcagg aactccgcag aatcctggaa gaagtgtccg
    10141 tagattttct ttttgtatgc ggcaaagcca gtgctgccac gattcatacg ggagtattta
    10201 tctgtggtaa taatctcctg gatctcaccc ggccgtgccg acataatgac gattcgctca
    10261 cccaaataga ttgcctcgtc aatgtcgtgt gtgaccatga ccatggtgac tttttcctcc
    10321 cgctggattc gcaggatctc tttctgcatc tggattcgtg tcagcgcatc cagcgcaccg
    10381 aacggctcat ccagcagcaa tatggatgga ttcccggcaa gacctctggc aatcgccacg
    10441 cgctgggaca taccgccgga cagctgcgag ggataggcat tttcaaagcc tttcagtccc
    10501 accagttcca gatgccgtgc aatgtgttcc tctcgctctg cctttttcat gttccggatg
    10561 ccgaaaccga tattgtcctt gattttcagc cacggcagca gccggtgatc ctggaatacc
    10621 atgccgcact gtgcatccgg tccggaaacg gtatgaccgt tccgggaaac gctgccatcc
    10681 tggtaggata ccagaccgca gatgattttc aaaagcgtac tctttccgca tccgctgtgt
    10741 cctacgatcg tgacaaactc tccttttttt acggaaagat caatgtcctt cagcacctga
    10801 aacgaagtgc cgtccttgac gaacgatttg ttcagatggg aaaccgtaat gatgttttcc
    10861 tgttcgggtt ctttcattgc tatcaccttt ttctcctatg tgtcttaggg gtttcattct
    10921 cttggtgtga ttccattcta ccccaccgca agtgaaaagt ccagaaagaa tggtgacagc
    10981 gttatcattt tgcgtgattt tttccggaaa aacaggcaaa aaaataccgc tttcccggcg
    11041 gtacgagcag cggttttgtt tttttggaag gataggcggc aacgatgcca gaaagaaatc
    11101 tgcgaaaaca tgggctgttt ttctattctc ctatttcccc tacaggataa ccggtattct
    11161 atttttgaca acgttatcat ttgccttgac aagccgtatc ctgtccgcta taataaaata
    11221 tagaatacag atagatatta tcgggtatga ggtgagggga aatgtcgatc acaaaaattg
    11281 cagcaattac cggtgcatcc gtttctaccg tggcacgagt cctgcgggat ccggaacaca
    11341 aatgccactc catggaactg aaagaacgca tcttacagac cgcacgggaa atcggctatg
    11401 tgccgaatga ggcggcgaaa gccttgaaga gcggcagtgc gccgaaaaag atctttcnnn
    11461 nnnnnnngtg agatgctccg gctggtggaa atcgaggtgc gcaaaagcgg cggtctgatt
    11521 gaaaacatct ggcggcacgc cgtcttttcc agcgaaaaaa agaccgactt tccggaaatc
    11581 gaaaagtcgg ctgcacagat gtttcacgat gccaagcaaa agcacgatgg gctgattctc
    11641 attggaaaat gctcgcaaaa gggattgaag tcgctgaaac gctatgaaaa gaatctcatc
    11701 tccatcaacc gcaattccac cacttatgaa gtggatgagg tgctgtgcga cggcagaaaa
    11761 atcgcactga cggcagtgaa ttatctgatc tcctgtgggc atcaaaagat cggctatgtg
    11821 ggagactgcc acaacgagac acggtatatc ggctaccagg aggcactggt acaccacggc
    11881 attgcgccgg acattgacga tgtctacgac attacaccca acgagaaaaa cggctatcag
    11941 gctatggcgt atttttccca gctggaaacg ccgcccagtg cgttttactg cgccaacgac
    12001 attctggcga tcggaatgct gaaatatctg gcgcagagca aaaactggta ttatcatccc
    12061 tctgtcatct ccagcgacaa cattgtagaa tctcagtata ccacgcccat gctaacaacg
    12121 gtttccctgc cgaaagcgga aatggcgcat ctggcaattc agattctgac agaccgcatg
    12181 aacggcggac ataaggctgt ggtgaaaacg gagctgcagt ccaccctagt catacgccag
    12241 agcgtgcatc aatatgtttc cacggaaaat gagccggagt attatattta aggcagccgc
    12301 ttatttttcc tggtcagaac gctgttctgc attcgtttcc tttttttgaa acagcagcag
    12361 cgaaaacgta tttcccacag aaccgcaccg caggttcaga tgcccattct gtttcatggc
    12421 gatctctctt gccagataca gtccgatccc gataccctct gtgttcctgc tgttttgtcc
    12481 ccgataaaac cgctgaaaga tacggctgcg ctctgactcc ggaatcggat ctgcctggtc
    12541 ggtcacatct accgcagcaa acagccccag ttcccgtacc cgaatcgcca ccgtgctgcc
    12601 ggacggggca tatttcaccg cattgtccag cagattgaaa atcgcctccg cagtccagcg
    12661 ctggtcatgc accaatgtga tatcgctttc gctgtcatag gttagtgtga tatgccgctg
    12721 ttttgccttt acaaaaacat tcttcaccgc catcaacact gtctggttca gacttttctc
    12781 ctccggctgc aactgaatca agccgctttc cagccgggaa agctgaatca tgctctcgga
    12841 cagaaacacc agccggtcta ccgctgtccg cagcacttca gtatactgct ttcgctgctg
    12901 ttccgtcagt gccggttctt ccagcagtgc agtgtacagt ttcaaggatg cgataggcgt
    12961 tttcagctga tgtgaaaggt cggatatcag ccctttcaga ttttcctgcg cctgtcggga
    13021 atcctcattt ttctgctgca ggatcgtcac cagtttcaac accttgtcct gtaattttga
    13081 gaggactgtg tcctcattcc gtggaaagat ttcccgatgt tccaggaaaa gcaggcagtc
    13141 gcacaaatca gagagctgcg ccgcaacgct gcgccagtat gcctgttccc tatgccgctg
    13201 ccatgcggtg aagagaactt gcagcgcaga caaccccagc acaatacacg ccgcaggcac
    13261 attccgggtc agaaagaaca cacttccgcc gcacagcagg ggcagcagta tcgctaaaac
    13321 aatgaaaaag attcgatttg tctgcttcat tgggattctc caaaggtata gccaatgccg
    13381 aatactgtaa cgatcagctt tggatttttg ggatcggtct cgattttctg ccgcagccgg
    13441 cggatcaaga cacgcagcgt attctcatcc acaaagttcc cgtcacagtc ccagagccgt
    13501 tccaggatca gatcacgggt tagcacctgt ccccggttac ggatcagcag ttccagcagc
    13561 ttgtactcca tggcggaaag tttcactggc gcattctctt tctgcacctg cattttcaca
    13621 aagtccaccg tcaacgtcaa ataggagaac acgtctcccg atgcggcact ctgactgcgg
    13681 cgaagtactg caaggattcg ctggtacagc agttcgatgg aaaatggttt cgctatatag
    13741 tcgtcacagc catgctgaaa ccctgcgatc atctgagatt ccgtgtcatt tgctgtcaga
    13801 aagatcaccg gcgcagcgct gattttccgc agccgactgc aaagttccag accactgcca
    13861 tccggcagac gaatatccag caggatcaga tgataggacg atccggcaag cagtgtttct
    13921 gccctggcaa tggtaccggc acagtctgcg gcaaatccct ttccctgcaa ataaaggcga
    13981 ataccgccgc tgatcgtggc atcatcttct acaatcaaaa tacgctgcat ggtgcatttt
    14041 cccctttcgt tttttcaatt cagtatagca ttttccgggt aaaaagtcaa tttgcaaaaa
    14101 tcccttttcc gatatggaaa agggattttt ctcactccat gtgccgcagc cgtgcagtaa
    14161 tgctttcttt cgccgcaact cgataaacca gaatcgggac aaggatacaa atggcaaaga
    14221 tcaaagaaga aagtattgcc agccatcccc acggatagcg gtatacagca taatctgctg
    14281 tcttttccgt catgaatccc accagatata agatgccgct gcccagggtc agaatcagaa
    14341 ccaatgtgat tccggcgtag tagatgccct ccagcatcag cattcgccgc acttgtttct
    14401 gggtcatgcc cacgctttcc atcactgcaa gttctgtccg ccgggtaaag atgccggtga
    14461 gcatcacgtt gatgaaattg ataacgccga tcagaatcaa aataccagaa acaccgccgc
    14521 tcaggagtct gatcgtgaac atggaatcct ggaattcctg tattatctct gtgcgtactt
    14581 ctgtatggga aacgaccgga tacatttgcg taatgctgcg gatctgatcg tttaggcggc
    14641 tctctgcctt gtcgtcatac tgtatcccaa taaaattgat actgggcgta tcggtcagct
    14701 gctccagggc agtttcactg accagaataa aatccggtgc gccggtgatg ctccaataag
    14761 aacctatgtg cagtttgctg atatccccgt ttaatgcaca gctgcccact tccagagaaa
    14821 gcgaactgct gccgtatttg tcctgcatag taatcgtttt gccctgcatc tgttctgcct
    14881 gttccgctgt gctgacagaa ccgagaatgc actgcctgcc atccagaaat gctttcacat
    14941 ctaccggcac tgcggcattc tggttatagg ttttcaaatc cctttccgaa attgttataa
    15001 cagtggttgc gaaattctcc ggtgattcgg taaagaattt ttccagatcc tccagagagc
    15061 tgccataacc gttattttcc atgatcggct gatacaatgc tgcatcaaag gacagggtca
    15121 catcttcata ccgggctgta tgcactgcgg taatgccgtc cagcgcctgt atcttttgca
    15181 gcatttcctc cgcagaacga ttgtttttcc ggatctgcgc cgcatccgtt ccgtccgcct
    15241 ctgagccgga attgacatag atcgtatagt catcggacag gtaatatttt acatagtttt
    15301 ccagtttcat gctgtccgca aaggcatttg tggcgagaaa cgccatcgtc cccagaaaca
    15361 gggacgcaaa taccagtacc gcacgtttct tttcccgaaa gacattccgg aacgccatct
    15421 tcgaaagctt tccgccgtct gttccctttt ttggcttaat ttttgccggg cgctgtcctt
    15481 gatatttcat tgcctctatc ggggagatac gactggcgag cctggatggt ttgcggcagc
    15541 tgactgcaat ggtgaaaagg gcaaacagaa tcgttcccac ataaatcagc ggatgaaact
    15601 gcatggtcag cggcatggaa tcgtcaccgc tgccgaacat ccgcattgca tagggaactg
    15661 ccacaaagga tgccagcgtt cccaacagca ttcccacagg aatacccact acagagaggg
    15721 aaaatgcctg caaacgaacc atcttccgaa tctgtttcgg tgtcgttccc acagttttca
    15781 gcattccgta aaagcgaata tccttggtaa tggaaatgta cataacatta tagatcagca
    15841 ggtagccgct gaatacgata atcagaccga tcgtaccaaa cagaccggca atgagaacac
    15901 ggttggacac catttcattc agcatatccg aagtgtagtc aaagtcctgc ccgtcttgca
    15961 gctgaacctc tgcatctaag gaatctgaca gcttttcctg caatccgatt ttcgctgaaa
    16021 tggagagcct gccatctttt tccgctgtca gccccagctg gtcggcatag gctttggaaa
    16081 cgaatccctg aaaaccgtcc tgggaattgg tgtagtccgt aaaccaaccg gacagggtaa
    16141 acaatttggt ttcaccgtcc gtcagcaggg aaatttccat cccgattttg ggtgaagtga
    16201 tgtgcatggt ttctagcgca ttccgggaaa gcatcacctc tcctgcctgc gtggggtaac
    16261 tgccctgtac atcgcttacg gcaggggtgt agttcttttc aaattctgta gtgtcgtaat
    16321 agctgagcag aattgccgta ttctcttcgc cggaagtata agccgtccct gcctgtatct
    16381 gcaccccggc agcatacaga taacggtttt cctcaatctg ctgcacctgc gctgccatcg
    16441 gatggtcgag ataaatggaa gaacgtgttc cctgctgccg ggtcagcatg accaccatgc
    16501 cctctgccag acttgccgtt gcggatacgg ttttgtttca tagaacgaaa agcgattttc
    16561 cggacgatct cccggttgtt attttgcagc atcgccgttc accaccttgc cgtcttcgat
    16621 ccgaaccatc cggtctgcca tcagtgcaat ctcctcattg tgtgtaatca taacaatggt
    16681 ctggtgaaac gcctggctgg tcattttcag cagtgcgatc acatccatac cggtcttgct
    16741 gtccaggttt cccgtgggtt catctgccag aagaatggca ggctttgccg ccagcgcccg
    16801 tgcaatcgcg actcgctgct gctggccgcc ggcgagctga gtcggcaatg cctgcgcctt
    16861 ttctaccagc cccagcgtgt gctgaatggg
//
LOCUS       DAWENO010000028        16796 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1427_length_16796_cov_13.558449, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000028 DAWENO010000000
VERSION     DAWENO010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16796)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_16796_cov_13.558449"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    238..265
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3883 a         4082 c         5120 g         3683 t
ORIGIN      
        1 taattctata cagagatctg ggttggtata aacagaattt ttcttcttag aaatgatcgc
       61 tttaactttt tcatcaattt catttggcag taaaacacgg cagattgata ttttagataa
      121 atcatctatc gtacaaaagg aaggaacgga atggtcagaa gattctacag taagttgaaa
      181 atttggaaag gaaatagaat tacatttgaa ttgaattgtg cttttcagat attctttnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnaatgt tgaagataac ttctaaagaa ataggagtat
      301 acatatttgg tgcctcaatg ttactcatat gcgttcggct aattccgatt ctttctgcaa
      361 gctgtacttg cgtaagtttt tttaatttcc gttgatgtgc tatattgagt ccaatacggc
      421 gatatttctc gtaattgctt aatttcatat aatcagccat gaaaccacct cttcttttat
      481 tataacacaa agtttaattt gcaaaaacaa actgtagctt tgcaaaaata tagctttttg
      541 cattattatc ttgtaaaccc atccccattt atgctataat agaaacaaca ctttaggcaa
      601 tgtcacgcaa agtccgccgg acggctttgc gtggtgctgc cgcatcacaa acaagcgagg
      661 tgaaaagccg tgtccctgcg ccacggtgaa caatgaaacg attggttgtt ggcataactg
      721 cccatgttga ctccggaaag actactcttt ccgaggcact gctataccgc accggagaga
      781 tccgcaagct gggacgggtc gatcaccaga ctgcattcct ggacacccac cctatggaac
      841 gggaccgggg cattactatt tttgcgcacc aggcgagcat tgactacggc ggcgcatcgt
      901 atactctgct ggatacgccg ggacacgtgg acttttccgc agagaccgag cgcaccatgc
      961 aggtgctgga ctacgccatc ctggtcatca gcggcacgga cggggtgcag tctcacacgg
     1021 agacgctgtg gaaactgctg gaacgctacc acgtgccggt gttccttttc gtcaacaaga
     1081 tggatctcgc cggggcagac cgggcggcag ttctggaaca gctccagacc cggctcagca
     1141 gccgctgcgt ggacttttcc cgggacggca gtgacggctt ttatgagcag gcggcgcttt
     1201 tggaagagtc ctggatggac acctatctgg aacagggcag cattgcgccg gaacagctgg
     1261 cacggggcgt ggctgcccgg aagattatcc cctgttattt cggggctgcg ctgaaactgg
     1321 agggcgtgga tgaattcctg gaagggctgc accgctatac cctggaacag gcgtatccgg
     1381 cgcagttcgg ggcaagggtg ttcaagatcg catcggacga aaagggaaac cggctgagtt
     1441 atctcaaaat caccggcggc aggctccgtg tccgggacgg catttcctat gcggcatcca
     1501 acggcaagga gatgcaggaa aagatcagcc agatccggat ctatgccggg gcaaagtttg
     1561 agaccagaga cgctgcgcct gccggaacgg tctgcgccgt gacgggcttg agccggacgt
     1621 ttatcggaca ggggctgggg agcgccgccg acggcattgc gcccgtgctg gaaccggtgc
     1681 tgcgctacgg ggtgcagctg ccggagggtg tccatcccac agatgcgctg aaacagcttg
     1741 cccagctgga ggaggaagat ccggcgctcc atgtggtgtg gaacgatcac gccggacaga
     1801 tacagatgca gctgatgggc gaggtgcagc tggaggtttt gcagcggctg attgcccagc
     1861 ggttcggcat ggaggtacag ttcgacgccg gacagatcac ctataaagag accatcgctg
     1921 cgccggtgga gggcgtggga cactatgagc cgctgtgcca ctatgcggag gtgcatctgc
     1981 tgctggagcc gctgccccag ggcagtgggc tgcagttccg gacaagctgc cgggaggacg
     2041 acctggaccg gaactggcag cggctggtgc tgacccatct ggaagagaaa acccacctgg
     2101 gcgtactgac cggttcgcct atcaccgata tgcgtatcac gctctgcgcc ggaaaggcac
     2161 acgtgaagca caccgaaggc ggcgatttcc gccaggcgac ctatcgtgcg gtgcgcaacg
     2221 gactgcggaa agcggagagc gttctgttag agccgtggta tgacttctta ctggaactgc
     2281 ccaccgggaa tgtgggacgt gccatgaccg acttacagca gatgggcgca aagttccagc
     2341 cgccggagac ggagggcgac cgctcggtct tacagggcag cgcaccggtg gcaaagctcc
     2401 ggggctatgc cgctgaggtg accggttata ctcacggcat gggtcggctg gtctgttccc
     2461 cgaagggcta tgcgccctgt cagaatccgg aggaagtgat cgccgctgtg ggctatgact
     2521 gtgacggcga tctggaaaac acggcggatt ccatcttctg tgcgcacggc gccgggtatg
     2581 cggtgaagtg ggacgaggtg gaacagcata tgcacctgcc cagctgtctg acggcacagc
     2641 cggaggaagt ggatgtgccg gaaacgccgg tgcgttctgc gggcgcagcc tatcacggca
     2701 gcctggcaga ggacaaggaa ctgatggcga ttttcgagcg cacctatggg aagatcgagc
     2761 gcagtccccg gcaggcgctg tatacgccga aggaagagcc ggcgcagtat cacggaaagc
     2821 ctgacccggc gtatgacggc gaggagtatc tgctggtgga cgggtacaac atcatcttcg
     2881 cctgggacga gctgaaaacc attgcccggg acaacctgga cggggcgaga ggacagctga
     2941 tgcacattct cagcaactac tgcggctatc ggcagtgccg gctgatcctg gtatttgacg
     3001 catacaaagt aaagggacag cacggcgaaa cggagcagta tcacaacatc accgtagtgt
     3061 acaccaaaga ggcagagacg gcggattcct acattgaaaa ggcgacgctg gatctttcga
     3121 aaaagcacaa ggtgcgtgtg gcgacttcgg acggcatgga acagcttatc attctgggga
     3181 acggtgcgct ccgggtgtcg gcggaggagt tccggcagga ggtgctgcaa accgaaacgg
     3241 cgatacgggc gtatgcgtcg cagctgaaac aggggaaaaa gaccattacg gaaaagcaga
     3301 cgccgaagaa atagggtgca ttgcctgccg gtgcattccg gcgctttcgc tgaatttatg
     3361 ccccgaaaat aggcgctttt gtcaattgtc tgcatgggaa aaatatgcta taatgaaaga
     3421 aaaacaggcg ggtgtgggtg gtgactgaat ccgttttatg attggttaag ttgacggtgg
     3481 attacccctc agtcaagcct acggcttgcc agctcccctg ttaggggagc ctattgttag
     3541 gggagtctat attaaaaact acctcaagga caggggaatt catgctgcat tcgactctgt
     3601 gtgcatatac aaggagggaa cttgacatga aattggggaa aaggctgcgt gcggcagcgg
     3661 cgcttttgct gtcggcggtg atgctgctgt ggacgggact tcctgtgacg gcggcgtatg
     3721 agaacacgca caacaatacc ggaaagccct cacaggatat cctggcggcg gcggaatccc
     3781 agctgggcta taccactccg gcagacggcg caaagtataa ccattggtac ggggcagtca
     3841 acgggaatta tacctattcc tggtgtcaga cgtttttgtc ctggtgtgcg gagcaggcgg
     3901 agatcggcac ggatgtgatc ccgaaagaga cgtctgtttc tgccgcctgg ggctttttcc
     3961 aggcagacaa gcgctttctg aagagccagt cacaaggcgg cagtgatacg ccacgtgccg
     4021 gagacattgc cttttacagc gagaccgggg atgcggcggc gctgacccat gtggggctgg
     4081 tgaccggtgc ggaaaacggc acgctgtata ccattgaggg aaacgtctcc aaccaggtca
     4141 tgcgctgtga gcggcagctg gattcggtgc agatcatcgg ctatggctgt ccgctgtatg
     4201 cagatctgga aaaccggatc gtgccggaac tgccggtgat gacggtcacg cccggaatga
     4261 ccaaccttgc caccagcatt tcctggacgg catctgccca tgcgcagaag tatctgctgg
     4321 tgatctggga tgcagacggg aacgaagtgg tacgggagga gaccaccggc gtccggtttc
     4381 agaaactgct gccggacggg gattatacgg cacagcttac cgcagaaaac agcggaacga
     4441 ctgtcacaac ggaaaagcag gcgtttaccg tgacggagaa tcataatccg ctgccgtttt
     4501 ccgctgtggt gaatgccggg acggatgaag agccgtctac ggtcagctgg acggagtccg
     4561 tcggggcgct ttcctatcgg gtgtgctttg tgaatgcgga caccgggcag acggcggaga
     4621 cgctggaaac tgacaataca gtacgcacct gcgaaaagca gctggaaccg gggaattata
     4681 ccgtacaggt cacggcgtgc agcggcagct atgagaccgc atcgcctgtg ctgtcgttta
     4741 cggtgaaata ccggcgtccg gtgacagcgc ctgtgctgga agtgaacggc ggcacataca
     4801 acacgtccac cacgatcacg tgggatgacg gagagcgtgc cgtgggatac cgcatcagga
     4861 tcatacggga agaattctgc tatgaattca gccatctggt ttgtccgccc gtgggcgtgg
     4921 acagctcgct ggatctggta taccagaatg acatcaccat cgtggaagag gatatgggaa
     4981 tgcagcacag ctggtccggc gtgctgacgg aaggctttta ttgggtgtat gtcaccgcct
     5041 ataatgccgc cggagatacg gctgaggcat atcatagctt gaatgttcgt gtgccgctgg
     5101 aaggcgactt ggacgacgac ggcaagttga cgcagcagga cgctgcgctg ctggaacagt
     5161 atctgctggg cgatctggcg ttttctcgtt atggaatgac gctgcgtgcg gatatggacg
     5221 gagacggcag agtcaacggg ttcgatctgg cgctgctgcg gcagaagatc gcataaatac
     5281 agaaggagaa caggatgaaa ctgggaaaaa aactgcgtgc tgtcacagcc ggactgctga
     5341 cggcggcgtt ctctgtggga atgggtgcat tcccgggaac ggcgctggag actggcgccg
     5401 gagaacttgc cggattacag gcgtttctgc tggggaacag cggcaccggc ggggcagact
     5461 ataacggcga cggcgttgtg gacggcatgg atctgacgct gctgcggcag aaaaccggag
     5521 cgcccggcga gatacaggac ggctataccg gctttatcca cgcagacgga aagcagctgg
     5581 tggatgagga cggaaagccg tatacgataa agggcatggc gttcggcaac gaggtgtggt
     5641 cgaatccgtc ggcagaaccg acacggcatc acgatgcgga aagctatcag gaactggcgg
     5701 agatgggttt cgattctgtc cggttctatc tgaactacgg gctgttcgag tcggacagcg
     5761 atccgtatac ctaccgggaa gagggctttg cgtggctgga caaaaacatt gcctgggcaa
     5821 aggcggcagg catccggctg gtgctgaata tgcactatcc ccagggcgga tatcagtcgc
     5881 agggtgacgg cacggcgctg tggacagagc cggaaaacca gaaacggctc tgcgccctct
     5941 ggacggagat cgcccggcgc tatgcagatg aaccagtgat cctgggatac gggctggtga
     6001 atgaaccggt ggttgcggca gccagcggcg aaaagagcct ggaactgtgg cagtccgtgg
     6061 cgcagacgct gacggacggc atccgcacgg tggacaccaa ccacatgatt tttgtggagc
     6121 ggatgtgcgc atcccaggat ctcgccggga cacaggaaca gtgggtcaat ttcaacgacg
     6181 aaaacaacta cgtccggctg gacgatgaca atacggtgta tgagttccac tactacgacc
     6241 cccacgcctt ttcccaccag ggctttgact gggcagggac actggggaat gacgtgtcgt
     6301 atccggacga aagctatctg gtgtctggcg gcaataccca gtggagtacg tctacctttg
     6361 ccggcgataa ggcggacacc tccgataccg agtggcagta tctgaaaagc ggaaagatca
     6421 cgccgaaggc agacggtacg caggtcatca gcctggtgtt ccaggcggag aatgtgggca
     6481 gctatggcta tgcccgtgcg gatgaactgc gtctggacga atacgacgag gacggcaact
     6541 gggtgcagac catctatgcg gaggactgcg atgataccac agcgctgaac ttctggtcca
     6601 gcgacaagtc cggcggactg tactacactt ccggcgaggg acacttgaaa aagggctgtc
     6661 tgatgattac cggcaccaca gacgatgcca acggcggcac acggtatttc tgccccacgc
     6721 cgggacacag ctatgaggca agcggctatt tccaggtgaa cacgaagaac gccggggcga
     6781 tcgtgcggcc tcgtgtggat gtgtgggatg tggattccat cgacgtactg aaccgggcgt
     6841 atctggaaaa gaccattgcg cagaacattg cattttcgga caagtacaat gtgccggtat
     6901 actgcggcga attcggcgca ggcatccact gctttgagaa tgaccggggc ggtgaccgct
     6961 ggctggatga tgtgatggac attttccacc agaataatat cagtttcaac tatcactcgt
     7021 ttaatgaata ctcgttcggg ctgcacaacg gcagcggtct gccaacggcg gagaaccgga
     7081 acgacacctt gtaccaggta ctggtggaca aactgaaaaa atatgcagaa taagtgagag
     7141 gaatacaaaa catgacaaac gcagaaaagc agttaaagga aatggaagag cagatgcgcc
     7201 gggaacaggc gtcgcagaaa aaggagaaac agggcagatc ggcgaaaccc tatgccgtga
     7261 aaaaggagtc ccatccgctg gcatcgtttc tcatcgggct ggtgctgctg ggcgccggaa
     7321 tttactgggt gctgaactct ttcgttgttt cgttctcctg gggcagcctc tggggcggat
     7381 tcgggatgaa cacctcgctc gctacagggc tgatgctggt gccgcttttg atcggcatcg
     7441 ggctgttgtt cttcctggat cgaaagtgga tcggctgggt cgtggtcgcc attggcttgc
     7501 tggcgattct ggtgacactg ctgacctcgg tgcggtttcg tccaatcacg gcgtcgctgt
     7561 ggcaatatgt ggtcatgttc ggcatgatcg ccgccggctg cggtctggtg gcacggggct
     7621 tgctgaagag cagggattaa ggcaacactg cacaaagttt gtgcggtaat gaaaaggcgt
     7681 attgtcatgg gcagtacgct tttttcgagt ctgtttcata aggagtaaat gcagatgaaa
     7741 aagaaacttt cccgctggac ggcggttttt acggtgctgc tgctctgcat ggggctgtgt
     7801 tcggggctgc cggtcagtgc ggcgtatgag aacactcata gaaacagcgg aaatccacga
     7861 gttgacattg tggaggttgc aaagacgcag atcggctatc tggagggcag tctggaaggg
     7921 acagttaagg ggaataacaa ctacacgaaa tacaacgtgt ggaacggtcg gatcagcggc
     7981 tacggctcgg acggctatgg atatccctgg tgtcacacct ttgtgtcctg gtgcgccaat
     8041 caagccggca tcggtacggg cgtgattccc agaaccgcag ggacaggcac gggaagatcg
     8101 tttttcgtga gacaggggac atatcagcag agtgcggcga acggcggcag ctatgtgccg
     8161 caggcagggg acattatcta ttacggctcc ggctcttcgc cgtctcatgt gggaatcgtc
     8221 tccggctgca acggcagcac ggtctacgcc atagagggca actgctcgga tatggtcact
     8281 acgagagcca tcggactgtc ggatggctat atcatcgggt acggcgtgcc gaattataag
     8341 agtgtgaatc tgcccacgac ggcagttctg tccaaaaatc agaattggta tgatatcagt
     8401 gatactgttg ttttgacggt atcttcggat aatgctacaa attatttttt gtctattaca
     8461 aaagacggaa acagcgtagt gcaaggaatt gagatacctg gaacatatag catttcagca
     8521 gctgatttgg gctatgggga ttactatgcc tgggcatctg ccgccaacag ttctggtggt
     8581 gttgattcaa atggtatcac tttttctatt gttggcggag cggcttattc tagcgtggaa
     8641 acagcgaaaa gtatgtattc actgacagat actgtttcaa tttcagttag cacaatttgt
     8701 gcaaaagggc aagtaatcgg aattgataaa aatgatacga caagagttgt aacagaagac
     8761 tgtgattcca catatcaaat tagtgccagt caacttggtg ttggaaaata tagtgcctat
     8821 ttttctgttt ataatggttc aggtgctgtt gatacagaac gagttgcttt ctgcatctat
     8881 gcgccggagg atctgggaac ggatttttgt gctgaaatcg ttcatgtgaa aacggagaaa
     8941 atgctgactg atgcggcgga tgcgtctgtg gatgtatgca atgcggcagg ggatgcggcg
     9001 caggtatggc agttcataaa aaacagagac ggcagctata aaatcacaaa cagcaagacc
     9061 ggaaaggtgc tgtctcccgg cggcgatgca caagactggt atatctgcgg agaatccgga
     9121 gcgtattatt tgaaatcgaa aaatgcggat caggtgctta ctgtggcaga tgacagcgaa
     9181 aatgtgcaga tgaatgactg gaacgggact gccgcacaga agtttcagat acggaagcag
     9241 gcggtttccg gtgacctgaa cagtgacagc attctcaccg tcaccgacgc cgttctcctc
     9301 cagcggtaca tcctgggcga gaaaacgctg acgcagaccc agcggcaagc cgccgacctt
     9361 aacgcagacg gcgtggtcaa cgggtttgac ctggcgctgc tgcggcagaa gttggtggta
     9421 tgaaaggagt aggtgccgat gaaaaagaaa ctttcccgct ggacggcggt ttttacggtg
     9481 atgctgctct gcatggggct gtgttcgggg ctgccggtca gtgcggcgta tgagaacact
     9541 catagaaaca gcggaaatcc acgagttgac attgtggagg ttgcaaagac gcagatcggc
     9601 tatctggagg gcagtctgga agggacagtt aaggggaata acaactacac gaaatacaac
     9661 gtgtggaacg gtcggatcag cggctacggc tcggacggct atggatatcc ctggtgtcac
     9721 acctttgtgt cctggtgcgc caatcaagcc ggcatcggta cgggcgtgat tcccagaacc
     9781 gcagggacag gcacgggaag atcgtttttc gtgagacagg ggacatatca gcagagtgcg
     9841 gcgaacggcg gcagctatgt gccgcaggca ggggacatta tctattacgg ctccggctct
     9901 tcgccgtctc atgtgggaat cgtctccggc tgcaacggca gcacggtcta cgccatagag
     9961 ggcaactgct cggatatggt cactacgaga gccatcggac tgtcggatgg ctatatcatc
    10021 gggtacggcg tgccgaatta taagagtgtg catcctgcgc cgtcgcttgg cgtgacgccg
    10081 ggaaactcct ctacggaaac ggttttttcc tggaatgccc tggacggcgt tacaaggtat
    10141 gatttgaaag tgtggaaaaa ccaggtctgg gacggcgatg cgtactgggt agcatggggc
    10201 gcatcttcgc cttgttccac cgttctgccg gagggtacgt accaggcgta tgtggacgct
    10261 tgcttcgggg acgatgtgca gatgagcaat gtagtggaat ttacggtgaa aaagggctgc
    10321 cggctggaag tgcaggcagg aaattcgctg acgggaacgg cttttctctg gaacaccgca
    10381 gacgatgcca ccaggtatga cgttaaggtg tggaaagaca agatctggga aggcgatgcg
    10441 tactggatag aatggggcgc atcttcgccg tgttctgccg ttttgccgga gggaacgtac
    10501 caggcatatg tggacgcttg tttcggggat gatgtggcaa tgagcaatgt ggtgacgttt
    10561 tatgttgcgg cggcactgcc cggtgacctg gacggtgatg gtgtcctcac cgtcaccgac
    10621 gctgtactcc tccagcggta tctcctggga gcggacacgc tgacgcaggg gcaatggcag
    10681 gtcgctgact gcaacgcaga cggcgtggtc aacgggtttg acctggcatt gctgcggcag
    10741 aagttggtgg gatgatccaa tttgtattgc agggcagtgg gccgatgcgg catcgatctc
    10801 tactaacaaa aagaccgaaa ttctgcaaaa aacagttgac tttatgaaat gtttgtagta
    10861 tactaaagat atggaaatat ttttccgcaa tgacgcacac ataaatttgg gaaattttgt
    10921 gcagaaggag gaatttttta tgagaagaaa agaatggatg agagttgttg cggcacttgc
    10981 ggcgtttgca gtgctgggga caggctcggc ggtcacggcg gcagctgaag agaccggtac
    11041 tgtggtctcg gagacggaca gcacagcgga taagacggtg tttaccggca gctgcggtga
    11101 ggacgccaca tggacttacg acaaatccag cgaaacgctg acgattatgg gaacaggcag
    11161 cgtggaggca tacgtcaagg ctggtgttat gataaagacg ccgggctggc aggtggtcaa
    11221 tgattatgcg gcaaagcata tcgtgatcgg agatggcatc accgaagtgg gcgagggctt
    11281 tctggacacc tgcatttacg gcacaacaga acccagaaca ttacaggtca gcagtacggt
    11341 gaccaagatg gatctgaacc cggcgctcca ggagagaagt gtgacgtttt acggcacaaa
    11401 gggttcctgg ttctatgagc aggtctatga gaccaatcgt tttgtggaca tttccaccgg
    11461 tgagccggac aaggtcccga cttccggtac atattcggac ggcgcatcct ggaactatga
    11521 cgaaaaatcg gaaacgctga ccgtcagcgg tgacggcggc atcacagaga aagagtttac
    11581 cacttatttc ggctgggatc gcccggtaaa gagaattgtg atcgggaaag aggtcaagct
    11641 gccggactgg aattcggatg tcagcgggct gaatctgttt ttccgtgcgc tgatctcaga
    11701 tgcagagacc ggaaacggtc cgttcctgtc catctatccg gacagcgagg ctggaaaggc
    11761 ttatgaagat ctggtggcat atcgggtctc caagggcagt acggaagagg aagtaagaag
    11821 cggactggaa gaggtcgtgg cattcctgga tgcggatatc gcatataccg gggcgtgcgg
    11881 cgcagagggc agtgagatca cctggagctt agacccgaac accaatacgc tgactttctc
    11941 cggcaccggc accatccgca cctatgacgg acccaacgca aagtttgaga atccggcgtg
    12001 gaacgactgc aatgtgagca tcaaccacat cgtagtgggc gagggtatca ccgatatcgg
    12061 ctcgtttact gacgatatgt tctattataa ttcggacagc ggctgcgcat ggcaggatcg
    12121 gacaattgaa attccggaca gcgtggagag ctgggagcat ctttccccga acttcctgta
    12181 tgagaccaag tacggcacgg cgttctacta tagaaatgcc agccagaaat ccattcgtct
    12241 cagctgcagc ggtgtcacag ataacccggt atatccggta gaaggcacat cggcgctggg
    12301 cggttcgtgg cgctttgatt atgaatccag cacgctgaca atttccggac cgggatttgt
    12361 ggagtataag gatattgaga tgtatccttt caccaaggtc gtgatagaca gcagtgttac
    12421 cattggaagt acttatcagg atcaggattt cagcacggca ctgctgatgt ggttccagcg
    12481 gctgaatacc atttcggata cagaaccggg tcgtctggag atatcggtac tctatgacga
    12541 gaacagcgat ttccagcagg aatttgcaaa gctgccggaa tccgttcgtt ctgagacaca
    12601 gcgtgtttct gcactgcaca gcgttgcagt gggcgatgtc aaccaggacg gcagagtgga
    12661 tgtgaccgat gcggtactgc tcagcaaggc agttaacggc agtgtcactg tatccgatgc
    12721 gcagagagag accatggact gcgacggaga cggcgacatt acggcgaacg atgtgactac
    12781 gctgatgcgt ttcctggttc acctggaaac agaactgccg ccgaaacagt aacattagaa
    12841 cctgtcttca caagcgtata caagtgtctt ttcggcaaat tttgctgcat actgcgttaa
    12901 aaatacttgc cgggcgactg cgttcgaggc aggattctgc ttgagaacaa aggagtgtcg
    12961 cactaggatg gattgcgacc gacagcccgc ctgtgtattt ttgccttgtc tgcaacaaaa
    13021 ttatgctcga aaatccacat atatttcttg cgaagacagg ttcttattta gcgcctggcg
    13081 ttcagacgct ttcggatagg agaaaggcgc atcagaaatg gtgcgccttt tccgtatgcg
    13141 tcacagagaa aggagtttgt atgaaacaga aattttgccg gtggctggcg gttctgctgt
    13201 ctgccgtgat gctgctgtgg acggggctgc ccgtttcggc ggcggaaaat acctttgtgt
    13261 atacaggcag cccttctcag gatatcctgg cggcggcgga attccagctg ggctataccg
    13321 ctccggcaga cggggcaaag tataaccatt ggtacggcgc agtcaacgga agttatacct
    13381 attcctggtg tcagacgttt ttgtcctggt gtgcggaaca ggcggagatc ggcacggatg
    13441 ttttcccgaa agcggtttcg gtggcgagtg cctacgccgc tttcgccgga caaaagcgct
    13501 atcagaaaag cgctgcgcaa ggcggcagct atacaccacg tgccggagat gtagcgttct
    13561 acagcgaaaa cggggatgcg gatgcgctga gccatgtggg gctggtgacc gggacagcag
    13621 acggtaccct gtacaccatc gagggaaatg tttccaatca agtccagcgc tgtgagcggc
    13681 agctggactc ggtgcagatc atcggctatg gctgcccgat gtatgccgac ctgcctaacc
    13741 agatcgtgcc ggaagcgccg gtcgtgacgg ttgaccccgg cacatccgag taccgcacca
    13801 agatcgactg gacggagtgc gcagatgcac agcgctatct agtggtactc tgggacagcg
    13861 ctgggaaaga gctggtgcgg caggagagca cggactgcta ctattatgat tatctgacag
    13921 agggcgatta tacggtacag gtctctgcct gcaacggtga aaattatgcc gattcggaaa
    13981 aggtggcgtt ccgtgtggag gagcattctc agccaggtcg gttcgacatc caggtgctga
    14041 gcggttcggg aacgacgccg acccggctgc actggaagat cacagacggc gcagagggtt
    14101 accgcatctg catccggaac agcgtgaccg gggaaatcgt cgccgaagat gagctggggc
    14161 tgaacacgtg gtatccggtc tcactgcccg gcggcagcta tgaggcagaa gtgacggcgt
    14221 acaatccttg ttttgagcgg acggtgacaa agcagttcca gctcagcgcc gggacgcagt
    14281 tacagggcga tttcaacaat gacggcgttg tcaacagcgc cgatgtcagc gaactgcaaa
    14341 gctgtttgct ggggaagaaa gcggtttctg acccggcggc tgtggtgcgg tcggacatga
    14401 acggtgacgg cgttttgaac gggttcgacc tggcattact gcggcagaag ttggtgggat
    14461 aataaaaact cctccaccag ctacgctggt ccccctcgta ctgagcggtt ccctctgccc
    14521 tgcgggcatc tccccacccc gtggggagtc acctctttca gaggaggcac attcgctgca
    14581 acatctgttg cgcatcagta ggggcagatg cccacatctg cccgaacgcc atgccataat
    14641 attgggcgtt gcccctatgg ttttaccata accaacagaa aaagctgtac cccggagaat
    14701 ggttcgttcc ggggtacagc ttattttttc tattcgtgtt ctgttcgctc agcggatctt
    14761 tgcgccgacc ggcaggctgt catccgggaa gatgaccttg acgtcgccgt tgggcatatc
    14821 tgccgcacag atcatgccgc agctctctac gccgcacagc tttgccggtt tcagatttgc
    14881 taccagcatg atcttcttgc cgatgaggtc ttccggttta taccacggtg cgatgcccga
    14941 aaccacctgt ctgccgccca tgccgtcatc cagctggagc tttaacagtt tcttggactt
    15001 cttcaccttt tcgcattcca gtacctttgc cacacgcaga tcgacttcac cgaatttttc
    15061 gatgccgatc tcgtctgcca gcggctgggg cgtgaattcc ggttctttcg gttcttcctt
    15121 gggctgtgcc gccaggatca gccggttcag ctcgtcgatt tccttttcca cgtcgattct
    15181 cggaaaaatg atattgccct tgtgtaccgt cacatttgcc ggcagtgtgc cgaactggtc
    15241 tgcggtgctg tatgccacat ctgcctcgga tgcgccgatc tgctcccaga tcttcggcat
    15301 ggtctccggc atgaatgcgc tcagcggaac ggtgacgatg cggatggtct ccagcaggtt
    15361 gtacaggacg gttgccagac gggtgcggtt tgcctcgtcc ttcgccagaa tccacggtgc
    15421 agtctcgtcg atgtacttgt tggcacggga aactacctgg aagatctccg ccagcatacg
    15481 gttcagctgg gtctgatcca gcagtccctc cagcttttct ttcagtccgg tggctgcggc
    15541 aatcagctcg tcgtcgaact cgcccggctg acgctcttcc ggcagcgtgc cgccgaagta
    15601 cttgtccacc atggctacgg tacgggaaac caggttgccc agaccgttcg ccaggtcgga
    15661 gttgatgcgg ttaatcatga tctcgttgga gaaagagctg tctgcgccca gtgccatttc
    15721 ccggaggatg tgataccgga tggcatctgc gccgtagcgg tctgccagca ggaacgggtc
    15781 aaccacgtta cccagggact tggacatctt ctgtccgttg aaggtgatcc agccgtgaac
    15841 tgccagatgc ttcggcagcg gcagatccag cgccatcagc attgccggcc agataatggc
    15901 gtggaaacgc atgatgtcct tggcaaccat gtggacatct gccggccagt attcgtcgaa
    15961 gtcgtggtat gcgctgttct catatcccag agcgctgata tagttggaca gcgcatctac
    16021 ccacacgtag accacgtgct tggtgtcgaa ggtgaccggg atgccccagg tgaacgaggt
    16081 acgggaaacg cacaggtctt ccaggccggg cttgatgaag ttgttgacca gttctaccgc
    16141 acgggtcttg ggctggagat agtcggtctc cagcagcagc ttttcgatgc gctctgcaaa
    16201 gggtgccatc ttcatgaagt aagcctcttc ctttgcctcg gtgacctccc ggtggcattc
    16261 cggacagcag ccgttttcgt ccagctggga ctccgtccag aagctttcgc acggtgtgca
    16321 gtacttgccc ttatattcgc ccttatagat gtatcccagg tcgtacaggc gcttgaagat
    16381 cttctgtacg gtttctacat gatagtcgtc tgtggtgcgg atatagcggt catagctgac
    16441 gtgcattttt tcccacagtg ctttgaaggt cttaacgacc tcatctacat attctttcgg
    16501 cgtgataccc tttgccgccg ctttctgttc aattttcaga ccgtgttcat ctgttccggt
    16561 gaggaaaatg acatcatagc cctgcattcg tctgaaccgg gcgatagaat cgcaggctac
    16621 agtggtgtag cagtgaccga tgtggggatt tcccgacgga tagtagatcg gtgtcgtgat
    16681 ataatacggc ttttttgaca tacaaggtcc tccttaacat ttgtttgccc gaaggctcta
    16741 cctcttatta tagcacagtt ttactgcgtt gtccatgttt ttttgaaaat tttagg
//
LOCUS       DAWENO010000029        16650 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1447_length_16650_cov_12.866526, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000029 DAWENO010000000
VERSION     DAWENO010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16650
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_16650_cov_12.866526"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3663 a         4478 c         4614 g         3895 t
ORIGIN      
        1 acggaggtac aatatcatga tttcaccgta tccgcatctg atcgacctga acgattatcc
       61 tgtttcctgg tggaaacagg tggtagacct gggagagcag atcaagaatc acccggagat
      121 ctacgcaggc gcctgcagca acaagatcat ggcgacccta ttctatgagc cgtccacccg
      181 tacccagatg tcgttccaga ctgcaatgct gcgtctgggc ggcagactga tcggtttcga
      241 taacccggca aactcgtccg tctccaaggg agagaccttg aaggacacca caaagatcgt
      301 cagcggctat gcggatatcc tggtcatgcg ccatccggtt gccggtgcgg caaaggctgc
      361 ggcgctgtcc gcagactgtc cggtcatcaa cgccggtgac agcggtcacc tccacccgac
      421 ccagacgctg accgacctgc tgaccctgaa atgcgaaaag ggcagactgg atcatctgac
      481 catcggtctc tgcggcgatc tcatcaacgg cagaacggta cactctctgc tgaaagcaat
      541 gtcctgcttt gagggcaacc gtttcattct gatctccaca ccggaactgg gtctgccgtc
      601 ctatgtcaag gatattctca acgccagcaa ctgcgagtat gaggagatct cttctctgga
      661 agaggcactg ccgcagctgg atgtgctgta catgacccgg atccagcagg agcgctttgc
      721 cagccgtgag gaatatctgg cacagaagga cacctatatc ctcaccgtca agaagatgca
      781 ggcagcgaaa aaggatctga tcgtactgca tccgctgccc cgtgtagacg aaattgaagt
      841 ggctctggac gatgatcctc gtgccatgta cttcaagcag gcaaagtatg gtatgtacgt
      901 ccgcatggcg cttatcatca ctatgctgaa cctggacacc cacaccacac tgctgcacgg
      961 caagaccttc cccggcgcag cctgcaagaa ccccaactgc atcacctgta cagagacata
     1021 tctcccgaaa agctttattc cgctgggcaa ttctctgctg gaatgcgagt tctgcaacga
     1081 gcgcctgctg atggaacact gaatttctga ataagcgaaa agcatcattt cccgactgca
     1141 tacacgattg gactggcgtg cggcagtcgg ggaacgatgc tttttttgca caaatatatg
     1201 gctgtttcat gattgaattg tacaaattct tcacgaatcc ggtttatttt ctgtgattcc
     1261 ccttgcacaa aaatttgatt tgtgctatac taaaagggta gaaacttcct gtggaacggc
     1321 agaggtcacc ctgccggatg gaaccgcctt tcggcgatgc ggcagatgcc gaacgccgga
     1381 atacacagca gaaaccacag tccggtgaac ggcagacaga tctgcccata gaggtttagc
     1441 ggcatatcgc tgtagtccca cacgttccat cccatgacaa gattgtccgc aacgcccacg
     1501 gtgaattcca gcgccgtgat gagcagagcg ccgcagagac actggagcag ccgggtcttt
     1561 ggcggcgcag cggcacggat cagatagagt cccacggcgg cggcaccgcc ggtgagggtc
     1621 atggtccagt gggtatatcc acggcataca atttccagca gactataggt aaaagccccc
     1681 agcagaaaga accagcagag ttcatagggc ttgtgatgca gtttcaaggg atcacctctt
     1741 tgttttgatg ctgtctgtag tctgcgctga aaaccaaaaa atatacaaag cggcatcgcc
     1801 tgcaaccgaa aaaacgatgc tgtaactatc tgaggaggta tcacaatgcg aaaaagtggt
     1861 attttgatgc acatttccag tctgccgtct gagttcggca tcggcaagct gggcgattcg
     1921 gcttatatgt ttgccaactt cctgcgggac tgcggtgtcc aggtgtggca gatcctgccg
     1981 ctttcgccta ccagctacgg cgattctccg taccagtcct tctccgtcca cgccggaaac
     2041 ccctacttta tcgacttcca gcgcctggag aaaaagggct atcttgcgcc gccggactat
     2101 gcggatatta actggggcga tgatccccgc cgggtcgact acgccagagt gtataactac
     2161 tgtttccacg tactccggac cgcatacagc cgtttccgga aggacgatcc gggctatctg
     2221 accttctgcg aggcacaaaa ggcatggctg ccggaatacg ccctgtttat ggcgctgaaa
     2281 gatgcccatg agggaaagcc ctggtatcag tgggaaccgg cgctggcgca gcgtgactcc
     2341 aaggcactgg aaaaggcaaa ggagacctat gccgaccaga tcgacttcta cagcgtgctg
     2401 caatactggt tctaccagca gtggggcgaa ctcaagacct actgcaatct gaacggcatt
     2461 tccattgtgg gagatattcc catttatgtg gcatatgaca gcgtggatgt ctgggcaaat
     2521 ccggagcttt tcctcctgga caaagagcga aagcccatcg acgttgccgg ctgtccgccg
     2581 gatgtctttt cgcccaccgg tcagctgtgg ggcaatccgc tgtatgactg ggcatatcac
     2641 aaggagaccg gctttgcctg gtggatcgac cgcctgaaga gcgcatccac gctgtacaac
     2701 accgtgcgca tcgaccattt ccgtggattc gaaagctatt atgccatccc ttacggcaac
     2761 aagaccgcag agatcggcga gtggcggaaa ggtccgggca tggcgctgtt cgatgcggtc
     2821 aaggcatcgc tgggcgatct gtccatcatc gccgaagacc tgggctttgt gacgccggaa
     2881 gtgcgcaagc tgctgaaaga ctccggctat ccgggcatga aagtactcca gttcgccttt
     2941 gacgacgacc ccaagagtac ctatctgccc cagaactttg agacttccaa ctgtatcgcc
     3001 tatacgggga cacacgataa tatgacgctc cgtggctgga tccacggttc gccgtcaaag
     3061 accatcgctt tcgccaagcg ctatctccac tgccggagcg caaacgatct gccggcagcc
     3121 atcctgcggc tcacctggag cagcacggca aagcttgcgg tggcacagat gcaggatttc
     3181 ctggatgccg gaccggaggg cagaatgaac acaccgtcca ctaccggcgg caactggcag
     3241 taccgcacac tgaccagcga ctttaacccc agactggcaa agcggatcta ccagctgaat
     3301 gaactgtaca accgcctgcc ggaggagaca gcgcccaagc cgctgtcgaa aaaacggcag
     3361 aaagcagcag aaaaggcagc agagaaagct gctgcggtaa acgaatccat gaaactgaag
     3421 aagaaggaga agaagtcatg atgaataagg aagaactgaa aagcggcatt ctgaacgccc
     3481 tgcacggaat cgaccccaag acggcaactc cgcagcagct gcaccaggcg ctgggtgaaa
     3541 ccattatgga ggcaatcgca ccggactgga acaagagcat ggacgcacac gagcgcagcc
     3601 gccgtgcctg ctacttctcc atggagttcc tggtgggtcg tgccgtttac aacaacctgc
     3661 tggtgctgga tgtgatgaaa gaggctgagg ccgcttttgc agaactgggc gtttcgctgg
     3721 cagacctgga agttatcgaa gatgccgctc tgggcaacgg cggtctgggc agactggctg
     3781 cctgcttcct ggacagcgca gcaacgctgg atctgccgct ggatggctat ggcatccgct
     3841 acaagtacgg tctgttcaag cagtacttcg aggacggatt ccagaaggaa gtgccggacg
     3901 actggatgcg ctacggcgat gcctggtcgg tgcgccggga cgaggatgct gttctggtgg
     3961 aattcgaggg acagaccgtt cgtgccgtgc cgtatgatat gccggtgatc ggctgcaaga
     4021 ccaggcacat cggcaatctc cgcctctggc aggcagagcc ggtgcataca ttcgacttcg
     4081 acctgttcaa tcagcagaag tacctggagg ctgccgctga gaccgtctat gcagaggata
     4141 tctcccgtgt tctctatccg aacgacgaca cctgggaagg caaaaagctg cgtctgaaac
     4201 agcagtattt cttctgcgcc gcatcgctcc aggacattct gaaaaagcac aaggcagttt
     4261 acggcacgct ggacaacctg gcagacaagc ttgccatcca gctgaacgac acccatccgg
     4321 tcatctccgt gccggaactg gtgcgccagc tgatgctgcc ggaaaacggc tataccttcg
     4381 accaggcatt cggcatggca agaaagatct tccactacac caaccacacc gttatgccgg
     4441 aggcactgga aaagtgggac tgcaatctgg tgcagcagct gctgccggca gtctatgata
     4501 tcattctgcg catcgaagag cagtttatga ccgagatgta ccagaagggc gtggacaagg
     4561 cgcaggcaaa ccgcatgaaa ctggtgcaga acggcatggt gcatatggca catatcgctg
     4621 tgtatgcgtc tgcgcacacc aacggcgttg cggcgatcca caccgagatt ctgaaggact
     4681 ccgtgctgaa agactggtac caggtttatc cggagcgttt ccagaacaag accaacggca
     4741 tcacccagcg ccgctggctg gcactgtgca acccggaact gtccggactg ctcaccgagc
     4801 tgctgggctc tgacgactgg aagattcgtc tggacgagct gaaaaagctg gagcgctatg
     4861 ccgatgacac cgctgtgctg gagcgtttcc tggcaatcaa gcagaccaaa aaggaacagc
     4921 ttgccgcata tatcgcagaa aaagagggcg tacagatcga cccgaattcc atcttcgaca
     4981 ttcagatcaa gcgcctgcat gagtacaagc gccagctgct gaacgcattc tccatcctgt
     5041 acctgtactt cggactcaag gacggcagca ttgcggatat cacaccggtg accttcctgt
     5101 tcggtgcaaa gtctgcgccg ggctatcgcc gtgccaaggc aatcatcaag tttatccacg
     5161 aggtggcaaa gctggtagag gcagacccgg tagtttcaca gaagatcaag gtggtatttg
     5221 tctccaacta taatgtttcc tatgcagaaa aactggtggc tgcggctgat gtttccgagc
     5281 agatctccac tgccggcaca gaggcatccg gcaccggcaa catgaagctg atgctcaatg
     5341 gtgcagttac cctgggtacc tatgacggcg caaacattga gattgtggaa gaggcagggg
     5401 aagagaacaa ctatatcttc ggcgcaaagg tagaggaact ggaaaagatc atgccgacct
     5461 atgacagccg caagctgttc tctgagaatg caaagatccg ccgtgtggta gagacgctga
     5521 tcgacggcac ctgctgtgac ggcggcagcg gcgatttccg ggagctgtat tactcgctcc
     5581 tggacggcgc aagctggcac acaccggata actactatct gctgggtgac ctggaatcct
     5641 acgtggcagc caagctgcgc tgcaacggcg actacaccga ccgcatggca ttcgccaaga
     5701 aacagtggct gaacatctgc aatgccggaa agttcagctc tgaccgtacc attgcggact
     5761 atgccgaaaa catctgggat atccagccgg tcaaggtgga ctgacagaga ccgtactgca
     5821 tgggaggtgc tgaaacgtga gtggatggat cgcatttggc tgctttcttc ttctgggaat
     5881 cacagatgct gtatttgcgt ggaaactgaa cggtgagccg tgggcgtttc tgatggctgg
     5941 tgatccgaaa ctgcccgacg gagaatatgt ctatgtgccg tcccgctttc aacaggcagc
     6001 aaaactcctt tgtctgttct atgccgttgt ctttttcctg tcggcattgg cggcactgct
     6061 gacgcaggcg ctccgggtgc tgcccagcgg ctggatggca ggaatactgt accttcatgg
     6121 tgcagttctg acgctgatcg cctttggaat gctggcatat ctggacagct actgtcagac
     6181 gaaaaatgaa gttctggtag aggcagaaga gggctaaaga aaatttaatt gtacaagaaa
     6241 acgccatggt attgctgcca tggcgttttt gtatggattc gtttttgaag attccggttt
     6301 acttcatttt ctgaccgcag gaaccgcaga actggctgtt cagcgcattg ggtgcgccgc
     6361 agtgcggaca gaactttgca gttgcagagg catctgtgtt gtctttcttg ctgtcgccgg
     6421 tcatttctgt gtagtggaat tccggctgtt ccgctgccgg tgtttccaca ggcgcagccg
     6481 gcttgtcggt gctcttttgc agtgccgctt tcttttgctt tttcttgacg gcggcgttgg
     6541 tgaaaatgcc gatgagcgca aagatcacgc cgtatccggc ggggatcagt gttgccagaa
     6601 ctgtgggctg taccagcgtc gggagtccct ttttcgtgaa gagcagggca tccggcacat
     6661 gaaacgcctc aaccaaaagc agggtcacgc cgcagcctgc cagtaccagc agtccgggaa
     6721 tcgccgcagc ccagccgtag ccggcaagca tcctgccctt gcgccagtgc tttttcactg
     6781 caaggatcag ccacaggaca aagcagccaa tggtcagaac gccaaaggtg atgatgccgg
     6841 acattccgca cagcatccgt gcggcgaact gcttgaaacc gctgccgacg gcgctgtcca
     6901 gggcatcgtt ccaggcgttt atatcatcgc ccatagtcca ttccagagac tggcggtctg
     6961 cctcagcaaa aacaacgccg gtctctttcc gcatggtgct gcccaggtcg ttctgcatca
     7021 gatcggcgat atcctccacg ctcgctttcg gcagttcgga actgtcgccg gtcagataat
     7081 cgctgaggtc atccagatag ttgcagaaaa gggcgttgaa ctccggctgc tgtaccagca
     7141 catcggcgta ttcttccgtc agattcggcg catcccagaa gtaccagtca tagagacact
     7201 ttcccagctg ttcggttttg ccgttgtctt tcagctgtac ctgagacagg tccatctggg
     7261 aaatggagcc ggaaagggtg tgctggggca aaaggctgtg caggatgctg ctgcaaagca
     7321 gggcaatgct gaaaagcagt gtcagcagaa acagcaggat cgagccgaac actgcgccgg
     7381 cacgggacgg ttgtttcgcc ggtttcgcct ttttttccgg agcagcgtct gctgccggtt
     7441 tggatttctt ttttgccata tggattcctc ctattgtagc ggttctgttt tgtttctatg
     7501 aagaaaatta tagcatagga atgcagtggg aatgtgtatt ttttgaaaac tttaccggat
     7561 acactgcatc atcggcgatt atgtatactt ttcccagctg cggatgcgct cccgcatttc
     7621 gtcgatgccc agcacgccgc aggcaaatcc ctttttgata aggatatccg gcaggtattc
     7681 gtcatacttt tcgaacatgg gaatctcctt gggatagacc agctccatgg gatcaagggg
     7741 cttgcgctcc tgttccagca gcacctggaa aaaggtacgc ttgtccgctt tcatcttgaa
     7801 ctggatgaaa taaggtctgg acggggacag cccggtgagg gtgagggatt cggcgcagcg
     7861 tatcttgaga aagcgctcca tggcgagaaa ggcgtacgtc cccagccagg ggaacagaca
     7921 ccacatatcg ccgcccagac agatcagcgg ctcttccgtc agcccggagt tctgcgccgt
     7981 gtgccgtgcc agctgtaatc gtgccgccgc attctgcatg agatagggat actgcctgga
     8041 ttccagcagc acccgtttca tgcgttccag aatttgggtg tggatatcgc cggggcattc
     8101 gccgaagtac gccggcactt tgcccttgat ctgttcgcag tataccgtgt gccgcttgtg
     8161 gtcgatctcg tccaccaccc agacatgacc ggcaatggcg atcttttcgc ccaccggcgg
     8221 cggctgaacg atcgttccca gctcctggga gttccaacgc acggtgtatt ccacattttc
     8281 ctggaacacg gcgtagaact tataggaact ggtgagccgt tcgccggcaa tgcccacgat
     8341 gagaccgccg gtctcagtgc gctggatgtg gtcgatctcc agcagatgcc gcagcaggag
     8401 cttgaaatcc tcctgggaga cccggtggaa atagctgagc gtcagcacac gggatgccag
     8461 tgccgccggt gtcatttcgc cgcaggatgc cagggtacac atggtctggt ggtagagcag
     8521 gctgtagggc agccggtcca gccgtggcgg ctcgacccag cgctcttcca gatagacctg
     8581 taccagggca atgccctgta agagtttcca cgggatggtg gtgggcaaaa gcgcccgtgc
     8641 ctccggcgga tcttcccgta tgacgaacca catctccggc ggcagatcac gccgtccggt
     8701 gcgtcccatg cgctgtaaga atgaggagac cgtaaagggc gcatccacct ggaatgcccg
     8761 ttccagtctg ccgatgtcga tgccgagttc cagggtggca gtggtgactg tagtcagaaa
     8821 caggtcgtcg tccttcatgg cagtttccgc cgtctcccgg taggcagaag acaggttgcc
     8881 gtggtggatg agaaaccgat ccggttcgtg gcgtgcctcg cagtactgcc gcagggtcgt
     8941 ggtgaccgcc tcgcattcct ctctggaatt gacaaagacc agacactttt tcccacgggt
     9001 atgctcgaag atgtagccga tgccggggtc tgcgtgtgcc ggggcagtgt ccgttttggg
     9061 ggcaagctcc ggcagtgcct gttccggcag atgctctgcc gctgcctgct gatcctgcac
     9121 atagaaatgt tccatggaca gccgccagcg cacgcccttg ctttcgatgc gtgggatcac
     9181 cgtgcctctg cctgttccgg cagccagaaa tgcgccggtc gcctccagat cgccaatggt
     9241 ggcagaaagc ccgatgcgcc gtggattcac cccagccatg cggcagagcc gttccatcag
     9301 acaaatcgtc tgaccgccac ggtcgccacg catcagcgaa tgcacctcgt caatgactat
     9361 gtaccgcagg tcgtgaaaca gcttggtgag tgcggcgtgt ttgtggagca gcatcgcctc
     9421 cagagactcc ggcgtgatct gcaaaatgcc agagggatgt ttcagcatct ttgccttgtg
     9481 ggactgggaa acgtcgccgt gccagtgcca gacagggatg ccagcctcgg cgcagaggtc
     9541 gttcagccgg agaaactggt cgttgataag cgctttcagc ggtccgatgt agatagcgcc
     9601 cacagaggtg ggcgggtctt cgtaaaactg agtgaggatg ggaaaaaacg ccgcctcggt
     9661 cttgccggag gcggtggagg cggagagcag gacattttca tccgtgttga aaatggcatc
     9721 gcctgctgcg acctggatgg cacgcaggga ttcccagtcg ttctggtaga taaagtcctg
     9781 gataaatggg gcatagcgtt cgaaaatgga catggggaaa cctcttaaat gtcaaactcg
     9841 gcgaattccg gtgcgactgt gttgtcgccc tccggctgtg cctgggcata ggaaaagctg
     9901 tcggaccgga gcagttcgga gacgttcagc tgcggattct gatagacgat gtccagcaat
     9961 tcgataaagt cccggatcac ctcacggggc gtgatgtggg agtctgcgcc gatgcgtccg
    10021 aattcgatct gcaggaactg gatgcgttct tcctgggtca gcgtcggcgt atagccgaac
    10081 agctgggcat ggatatccgt cagcttttcg gtcagcacca gcagttcctc gttggtcagc
    10141 gccgtcagat ggatcaccgg ggcaagcata tcctttacgc cctctcgtcc gaaacggcct
    10201 tccgccagcc gggagcgcag cgcctcatag ctgtatacgc cacggcgtgt gtcctcgatg
    10261 cactggggtg tgccgcccat gatgatgccc agatactgcg ccttgccctg gagcgtgtcg
    10321 ttgtacatgg tgaggatctt ctcgtagtta tactgccggg tgatgctgtt gggaaccttg
    10381 tagatgttca ccagttcgtc gatcagcacc agcataccgc tgtagcccgc ctttttcagg
    10441 aacatggcaa acagtttgat atactcgtac cagtcgtcgt cactgataat gatattcacg
    10501 cccagttccg acttcgcctc ggtcttggtg gtgtattcgc cccggaacca cttgacgacc
    10561 tttgccttgc tttcgtcgtc gccgtcccag taggcattat aatagagtgt cagcagcttg
    10621 gcaaagtcga atccgtgtac catttcatgg agcgtgccga tgacctcgta gatctttcgc
    10681 tccacagctt tccggaacag gggatcgtct gcggaaagcc cggactccgc tgcggtctcg
    10741 ctctgtacgc tgctgatcca gcggtcgagg atcagcgtca gtgcgccgcc gtccggacgg
    10801 gtcttggtgg acatattccg gatcagttcc ttataggtcg ccagtccctg tcccttcgtt
    10861 ccctggagcc gccgctccgg ggaaaggtcg gcatccacta ccacgaagtt ccggtccatc
    10921 acatagctgc ggatggtctg gagcagaaag cttttgccgc tgccgtattt gcccacgata
    10981 aaccggaacg atgcgccgcc gtctgcgata atttcaacgt cgtggagcag ggcgttgatc
    11041 tcctgttctc tgcctacggt aatatagggc agtccgatgc ggggaactac gccgcctttc
    11101 agcgaattga tgaccgcaga tgcgattcgt tttggtattt tcatagtgat cgttcctttc
    11161 tatgcggttt cagacagacg tttccagcaa tgctgcaacg tcttcggcgt aatcctcgat
    11221 cagttccggc gtgtcaccgt caaagaggat caccgtgtcg ccgaaatcat cgaacaaagt
    11281 ctcgttgatg tggtctacca ggacggacag catcacattg cgctgccgga gcaggtcggc
    11341 gtagggtgtc ccgtccagga gacagcgcag gaatgcagtc tcagtctcgc tgagccggct
    11401 gtcctgagcc ggcgtgtgtt ccgactctgg cgcaggggtc ggtgcggcaa taacgggcgg
    11461 ttcgggttcc tgggtcacat cttcatccac cagcagcttg tcccgtgtgg tatctgccgc
    11521 ctggcggatg ttttgcagcc gggaaaggtc gaactgtatc ggcttgggtt tcggcgctgc
    11581 tgctctccgg cgctcttcca gaaattccgc cagcgccttg tccagtgcct gctggatata
    11641 cttgggcagt tctgtctctt tcagcggatg cccgtagtcc gtcagaatgc gcagcttttg
    11701 gtcgatggtt ttcagaaaca gccccaggcg tttgctgccc ttgctgtagt cgaaataggt
    11761 ctcacacgtc cactcaccgt tccggcagtg ataggcgtgg caggggctga ggacaaagtc
    11821 gcagttcttg tgcgggtctt gttcgaaaaa tacggcgttg gtgaacatat gatagggcag
    11881 ttcgctctgc ctgccgaaga gatattccag gaaggaagtc tttttgtgtt tctcgaagta
    11941 gacgcacagg gcgtggaaac agaaaacagc cccttgttcc atcagccggg tctcgtcccg
    12001 gtagaatcgg gaacggacaa tgctttgccc ggacagggca gacagcgcag aaaagatctc
    12061 ctccttgctg tgttcggact cgtccagcag tacggggaag taagtgtcaa acgccgtgcc
    12121 gtgaccggcg tcgaattctt ccagcagtgc ggcgtccatg ccgtagtaga tcacatagtc
    12181 gtgcatccag ctggaaaaat agcacaagcc gttgtgcgga tcgacctttc cgtagtcgta
    12241 gtagaactgc cgcagcatct gaaagccggt ctccgggtcg ggtacgccga tctggttcag
    12301 cagttcaaag cagtacacct cagcgtagga aaggggaatg tgttccacaa tgccccggcg
    12361 tacctttgtc cgccaggtga aataggtgcg cagctggcgg tcgctcatgt cccggtaggt
    12421 ggggtagtag gagaagaaat ctgcgctttc gggtgcgttg tcggtgaagt cctccagcag
    12481 ctttgcctgc cgcagaaaca attcgttgta ggaatagtga tactggttcg gacgctgcgc
    12541 cagctttttg gcatctgcca gcggtttggg caggcggcgt gccatctgcg ccgccgtccg
    12601 caggatgggt tcgtcgtgat attcgctctg tatcttcgat gccacatttt tcagtttggg
    12661 gttgtccagg atctctgcaa tcagatccat gatatccggc atagcgccgc ctccttccgc
    12721 acaaaatact ctttcttcaa tataccatat tttatcggaa aagtcaaacc acagcgaaga
    12781 aaaagagcag acaccgcacg aaacggcatc tgctctaaag gcaataccgt acaaagcccg
    12841 cctgacggct ttgcacgcaa tctgcttgct gattttccgc catattgccc agaaatccct
    12901 tgccagacga ctgcgttcga ggcaggattc tgcccgagaa caaaggagtg tcgcactagg
    12961 attgattgcg accacaaagt ctgcctgcgg aatttctatg caatctgtcg aaaaatccga
    13021 ctgcgcatct tacgcacaag ctttgtgcgg tattgccttt ccaaatattt tggtttagtc
    13081 tgcgctcgga atggagttga tgatgtgtac caggaacttc agcagtacca cagcatcgtt
    13141 tgcgccgacc tcgttgctgc cgtcgcagtc tgcgttccgc tttgcggtgt catccagtgc
    13201 cactgcgcct gcgactgcct tgttcagcag gacagcgtct gtgatatcca ctctgccgtc
    13261 cagattgacg tcaccataga ggacatcgcc catcacgccg gtggaagagg ttgtcgtggt
    13321 tgtggttgta gtgacggatg aagtttctgt tgtgttctca tgatcttcaa cagtaacctt
    13381 gaaagaaacc tcggcaaagg acaaccgata gttcaagatc aaatccgagc ttgctttcac
    13441 acgaatggta tatgtgcccg gcttggaact gtcaaattcc gaagtgtcta tggtaaattt
    13501 atccggataa tcggcaggcg ataccttgtc atagatcaca tcacagctgt cttttccgta
    13561 atagtacttc agggaaattc gcagtccggt gagatccaga tcctccccaa cacgataggt
    13621 cagcttctcc ggctcgtcat ccagcagaag tgcgccctcg gattcataga taatatcgtc
    13681 tgaagtggtc gtcattgtag cttttgttgt cgccgtggta gttgtggtcg tctccgtggt
    13741 cgtcgtggta gacggcttgg ttgtggtggg tgtggtaacg tccggcttgg tgccgtccgg
    13801 ctctacgccc cagatcaagg tgtcgccgtc atacatggtg atatggtcgg tcgccaccat
    13861 tgcatcttcg ccgccctgtt ccagaccggc ataagaatag tcgtttgtgg gatcccatac
    13921 gccctggatg ttgtccggga tggagatgcg gaactggcat tcgctgcggt gttccgactg
    13981 accggtaggc atcactacgc tgccgtccgc aaaggacagc ttgacataat agatgttgcc
    14041 gtcatactgg atcgggtcag aaatggagat cttgcccttg tcgccgctgt gctggtcata
    14101 gccaaccttg acagagacgt cattgatgga atagcctgcg tctaccagtt cgctgatgtc
    14161 gaaatagtag ttgtaggaca ggttcttgat ggttcttgcc ggccatgcgc tgtggttcat
    14221 ggcgaatact ttcagctcgg tatagctgga tgcggactgg ttgatggatg ccttcatgaa
    14281 gaattcatcc cacttcggtg tctctgtcgg cgggaagtcc tggtcggatg tgccgccgta
    14341 tttgtctacc atagcgcaca gcgctgcggt gtagcctgcg ttgtagtcga tggcaacttc
    14401 ggtatactga tagtcgccga tcttgtcgga atagccgtcc ttctgatccg ggccgcccac
    14461 cagagcgcca tacagaatgt gtgcatgagg ctttgcatcc tcgccctcgg tctgaccgat
    14521 gttgctccac ttgtcgttcc atgcgccgct ggaggttctg tgatgccatg cctggggata
    14581 atcctcaccc atgccgacca cataactgaa attcttggag ttgtcgccga agcagtagtt
    14641 catgaccttg tcggcgaact tggtgtactt ttcagcctta gcggtgtcat cctgatacag
    14701 ctgatccact gccacatatg ccaggaaagc ggtggtggtg gcgtggcgga gcgaacccca
    14761 ctggaacagc cacggcagac cgtccggcgt ataggtgatc tgcttgccgc cgtaaccggt
    14821 ggtccagtat tccagatgct tggtgaactg atccttccac tgggattccc cggtgttgat
    14881 ggcatacagc agcataccgc cctgcatcac atcgtcccag cacatacccc aggtgtactt
    14941 catttcgctg gactgctctt ccttgcccag gttcgggata tagtcggtct tgcagagatc
    15001 caggtacttc tgctcgccgg ttgccatata cagccagttt gcagcgtaga acaggtcatc
    15061 gtagaatgtg gtgatcttgt agtaggactg ttcctccggg tcgtcgccga tggaacgcag
    15121 cttgtcggca cggtcgaaca ggtcaacggc gtgttccagg tattcctttg ccttttccgg
    15181 ctgggaatcc ttgaaattca gatagccggt ggcaagcgca gccgccatgt ctgcctcgat
    15241 gcagctgtcc tcacaggtgt agtacgggcg ctgggtctcg cccttcatca gctcgtactt
    15301 atccatatac acttccgcag aaccccacca gacgtggtcg aaggaacctt cgccgatcat
    15361 gtatacgatg ttgtcgccct ggtcgcagcc caccagataa tccagcgcaa actggaggtt
    15421 gttctcgtac atggtgcgct gaccagcctt gtctacgccg tcgccgtagt tcagcagacc
    15481 ccagcccagg agcgctgcgg aatacgcatt ggtcagcgta aacttcagat ggtcgccggc
    15541 gtcgaaccag ccgcccggca catagtcgtt tgtcatacag tcagagcgcc agctgacctc
    15601 gttccagtcc ggcttgacgc cgctctgctg tacctcgtag aaaaacatcg acttctggag
    15661 tgcttctccg taattgtact tgctgttgct gtccgcagca gtgaccacgg cgccaggaac
    15721 agcggatgcg accccggtaa acgccagcac agcggcggaa atcccggcaa tccacttctt
    15781 catagacaat catccttcct ttccaaaaat actggtacgc tttgtacacg agaaattgtc
    15841 caattatttt tattgtatca tatcttacat gggatgtcaa gattttttgt gtaacttgtc
    15901 aaaactaccg ccgaaaaaag ctgtcgaaga atcaaacacg cattttgcga ataagctttg
    15961 tgctgctgta attcttttat tctgtatagc taagaatttt tgatgatagg atgctgttaa
    16021 agtgagaagt gtttccgttt tttatttggg tataatcctt atcctcatac acatacaagt
    16081 gcaatgtgat ttcaccgtcg atttgtttta gatggctttt taagtcatta aattcctctg
    16141 atagattttc atgctgcata tattctgatt ttgaaatcaa caaattaaaa caagcagata
    16201 attttctgtt tttcgaagtt aatgaaataa acgtatccgc agtgaggtct gtagcatatt
    16261 ctgacggaaa gcatacttct gtgcagctat attctatgat ataatcatct aaaccggctt
    16321 gtaagatata cggatctatc cactgaatca ttttatcctt catcaatttt ccgtaataag
    16381 attcgttttt tacatataat tctatttctt tattgactcg ttttccgtat acctctgcgc
    16441 tgcattccgt gtagtactcg ccgtctgtaa tgctgacagt caaattataa tattgttctg
    16501 ctgacgaatc tggtcctaaa aaaacgcctc cggcaatagc ctgatgggta accgcagcct
    16561 gtacgtcata tttctctcta aaatatgctt ttacaacatc ctctgcattt ccctcaagtt
    16621 gtttcagttc agtttcagag atatgcgagg
//
LOCUS       DAWENO010000030        16615 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1452_length_16615_cov_12.061534, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000030 DAWENO010000000
VERSION     DAWENO010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_16615_cov_12.061534"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4168 a         4451 c         4043 g         3953 t
ORIGIN      
        1 gtatgtcaag aaatttctgt gcggtatgac ggaaaatcag caagcagatt gcgtgcaaag
       61 ccgtcaggcg ggctttgtgc ggtgttgcct gtaatatcgg taccggctta gcctttgact
      121 gcgccgtttg caatgccgcc gatgatctgc ttctgacaga agatatagaa gatcagaatc
      181 gggatcagtg ccagcagata ggaggcaaat gccaggttgt agttggtgcc gaactgtgtc
      241 tggaagttca gctgtgccag cggcagggtg gtatcttcgc tgctggacat gataaccagc
      301 ggtgtcataa cgtcgttcca ggtacccaga gccgtcagaa ctgccacggt tgcgtgcatg
      361 ggcttcatca tggggaaaat gatggtccag taggtcttcc aggtagaggc accgtcgatg
      421 tttgctgcct cttccattgc cagcggcacg tttttcagat agccgctgta cagcatgacg
      481 ttcatgggca tatagaatac cagatacagg atgatgacgc cgagacggtt gtccagaccc
      541 atcatcgcag tctgctttac cagcggcatc atcagaattg caaagggaac gaacataccg
      601 ctgacgatgt agaaatacac cagcttatag ccctttttcc ggttcatgtt tctgccgatg
      661 gcatagcctg ccatggagtg cagcagcaca gccagaacga tagtcacagc ggtaattaga
      721 aagctgttgc ccagggagtg ccagaagttt gtgacacgca ttgcctcaga gaagttgctg
      781 agactccagg aactggggaa cgaaagcgct ccggcaacat cgttcgtcat ttcggacgga
      841 ttcttaaagg cgatcatcac cgtcatatac aaggggaaca aaacggtgat acagcccaga
      901 aacagcagca ctgtcagaaa gatattcgta ccgccgattt tatgtgtctt tctcatagct
      961 gttcctcctt tttgccggtg atcttcatct gaatgatgga gaatgctaca attacaataa
     1021 agaatatgac tgcgttcgca ctctggaatc cgaactgacc gccgtccata ccgttcttat
     1081 agatcagata cgagatggat tcggtactct gtgccggacc gcctttggtc aaagacatga
     1141 tctggtcgaa taccatcatc atgtttttgg tgctgaggat cagattgatg gaaacgaagg
     1201 gcagcagcat ggggaatgtc agcttccaga acttctgcca cgggtttgcg ccgtcgatca
     1261 tgccagcctc gtagatgtct gccggaacgg tctgcaagcc ggaaatgtaa atgatggtgt
     1321 tcattgccac tgcctgccat acgccgacga tcagcacgcc gatccaggca tatttttcac
     1381 tggcgagaat gctgttttcc aggaatccga tgtgcattgc cttgcccaca gccgggataa
     1441 tataagtaaa gataaagctg aagatatagc cgacgatcag tccgcccaga atgttcggga
     1501 cgaagtatac gccacgcagc aggctcttgc agcggatcct gctgttcagt cccattgcca
     1561 gaagaagact cagcacgtta atcagaatcg tgccggcgat ggcatactta aaggtgaaca
     1621 gataggaatg cagcacacgt tcgtcggaaa acaggtcgat atagttccgc agacccacaa
     1681 agtcaaacga accatagcca cggtagttgg taaagctgta cactgcaccg gtaatcagcg
     1741 gcagcgtatt aaacaggaaa aacagaatca caatcggaat ggtcattgcc agaaacgtcc
     1801 ggttgttgct gctcaatgct tttttctgtc tcatgatgcc ttctcctcct catatttttg
     1861 tacttttgcg atgaggtcac ggttatagcg tttccattcc ttatcaaagc gcttgaggaa
     1921 tttcacggtg gaatcgctgc tgtcgtccat cagataggtc tgtaccatgg cgtccacgct
     1981 catctcgctg ggatagtggt gatcatggaa gtcggacatt ctgccctcat caatatagga
     2041 acgcattccg tccagcatag aaggcagcgt aaagtcgccc tccttgcacg gtacggcgtt
     2101 ctggtcgtcc agatagatct ggatggtctc atcgtcatac aggaactgca gaacttcata
     2161 aacggcatcc ttgtgcttgg tctccttcat cacgcagaac tgcaggtcta cgccggaatt
     2221 cagcacgttg tctgcctcgt cctcgtttgc cgggaacacg aaagagtcaa tgttcatgtc
     2281 cggattgacc gactggatct gcggcactgc atagctgccg atgggataca ttgcagattc
     2341 tccacgggca aatgcggtgc aggcgtcgtt atagctgtac gcatacggat tcggttctgc
     2401 atattccagc agagccttgt ctttttctgc gacctcctgg tacgcatcgc tgaaggtggt
     2461 ctttccggcg ttgacatcgc tgcacgtaga cggcgaggac aatccgactg ccagcgcatt
     2521 ccagggtgcc atacaggtcc aggtgtcctt atatcccagg tacagcggat acacgtccga
     2581 ttcgctctgg atctgctcgc acagcgccgt gaattcgctc caggtcgtgg gaatctccca
     2641 gccgttttcc tcgaacatat ccttgttgta caggatgccg gcacagtttg ccacatacgg
     2701 cagaccgtaa acgccgtcct tcgggataaa ctccagttcg tccacaatat ccatgtacgc
     2761 cggcttgatg tcctgcacgc cctcaaagtc cgaaacgtcc aggaacatat ctgcatccag
     2821 gaagttggaa tagttgatgt cgccgccgat gccgatgatg tccggataat cctcacggat
     2881 aaagcgtgtt ttcagcacgg tcattgcctc gttgggcgat tcaatggtga gatggatgtc
     2941 gtcgtgcgtt gcgttgaacc gttcctccat ctcctcgaaa gcggcaaccg cctccggctt
     3001 gtactgtacc attgtgactt ccgttttacc gtcattgttc gattttcctg cacagcccgg
     3061 cagcaaaagg gtagacaata ttgctcccgc cagaaccaga ctgcctgctg cattcttttt
     3121 tctcatgtcg atcttcctcc tttgtggtaa aacgctgcgt tttcgataaa tttattataa
     3181 catacccaga tgcgtcctgt aaatatcaca aatcaacttg ttttttgcca ttttgccgct
     3241 ttttcattca tttcggaaaa agatgttgca ttttggtata tttcgtgata taatgagtat
     3301 catatgaaca gccacaaaca gaacttgaac atcagcagac tttttgagag gattaaggta
     3361 aatttatgca aaactggatt ttctctattt tccccaatga caactttgtt gacctcggat
     3421 tattccagtt cgggtgggaa aagtgcgccc cggcgcattc cttcggaccg gcagccagaa
     3481 accactatct gtttcactat gtgctgtccg gcacagggac actgatggca gacgacagca
     3541 agggcgtaac gcagacctat tccatcaaaa gtatgcaggg gtttatgatc ttccccaacc
     3601 agatcaccac ctatgtggca gaccacgacc tgccgtggga atacgtctgg atcgaatttg
     3661 acggactgcg ggtcaagtct atcctggaca ccatcgggct gtcactggac aagcccgtct
     3721 atcacgcacg gaacaaaaac ctccggaaag atatggcaaa cgaaatgctg tacatcgcac
     3781 gcaacaagga cgcctcgccg ttccacctca tcgggcatct gtatctgttc ctggactatc
     3841 tgatgcgctc cgcagcagat gagcagatgg agagcggcgg aaagctccgg gacttctata
     3901 tccacgaggc actgacgtat atcgaacaca acttccagaa cgatatcacc attgaggata
     3961 ttgccagcgt ctgcggactg aaccgcacct acttcgggaa gatcttcaag gaggcactgg
     4021 gaaaaacgcc ccaggagttc ctggtgaatt accggatgct caaggcggca gagctgctga
     4081 agctgaccca cctatccatc ggagacattg gcatcacagt gggctatgcc aatcagatgc
     4141 acttttccag ggcgttcaaa aattgctacg gtgtttctcc cagggagtgg cgcaatcaga
     4201 accgcctgcc ggggatttta gaggacgaac ggggatgact ttgaaggaca aaaccgcccg
     4261 atttccagaa aacaactgga aaccgggcgg tttttcctat tcctcctatg cggcaaaaga
     4321 ggttttattt gcaggctggg tcttttttcg gaatgtccgc aaaagaaccg gaacagagat
     4381 cactgctgtg atgcactctg ccagcggaaa tgcccaccag acctgttccg caccgtgact
     4441 acctgccaaa aacagcaggc aaaacggaat cggcagcacc agcagccgca gcagtgccag
     4501 aaccagcggc gaaaatacac agcgaaatcc ctgtaagatt ccctggatca gtacagtgac
     4561 acccataaat acatagccga gcgtacagat ccgcatggaa cgcatacaga gttgatggat
     4621 ctccgggctg tctccgctct ggaacagtcc ggcaatgggt gacgcaaaga tctgaaacag
     4681 cagcgtaacc agcagcatca gtccggcggc atacaacaag ccgtagcgaa cgcactgccg
     4741 cagtcttgcc ctttctcgtc tgccgtaatg aaacgccacc acagtaatca gggtattgct
     4801 cagtccgaac gctgcaaaca gggcgaactg ctgtatctta tagtagatgc cataagctgc
     4861 ctgcagcggt tctgccatat ccccctgcgt tccaaataca agattgacga aaaggaccat
     4921 gacagaaagc agcccttgca tgatcgcagc cggaataccg atggcataaa ttccacgaat
     4981 cagtccccac tgcggacgaa tgtgtttcca gtcccgacga atctccttat cccagcggta
     5041 atggaacagc attgccatgc ccaaagatgc cagctgcccg acgactgttg cagctgctgc
     5101 gcctgtcacg cccatatcca gcccgaaaat aaagatgggg tccaggatga tgttcagcaa
     5161 tgcaccagtg atctgcgcaa tggtggaaag attggttctg ccggtcccct gcaaaaagcg
     5221 ctcataaacc gtatagccaa tgctgccgaa agagccgata cagcagattc gcagatactg
     5281 ctcccccatt tcctggcgga gtgcattccc gttcgcctgc attccgatga acgctctggt
     5341 tccgaaaatg ccgaacaaaa gaaaaaccgc ataaatacag acggcaagaa aaataccgtt
     5401 tccggcaatg caggatgcct tttcaaaatt gcgttctccc agcgtcttgg acagcagggc
     5461 gttgattccg ataccagttc ccacgcctac tgcaacaatg agcagctgga tggaatagga
     5521 atccgtcagc gccagaaccg ctgttgcgcc gttttccatg tggctgacaa aaatggtatc
     5581 cacaatattg tataccgcct gtaataccat agaaagcacc atcggcatcc ccatgctgag
     5641 gatcagtgtc cgtatcggcc aatctgccag cttattcggt tttccggctt gtaccgccgt
     5701 gttgtttttc tgcatatgtt tcatacctcc aaaagtaaat ctgcgcaaaa aaaaggcgta
     5761 aacccagtca aatactaact ggatttacgc cgtttcgcta cacgttataa gattattata
     5821 tcacagtgcg caaaaaagtg caagtgatat tttatgaaat tgtttggctc ttacgcctca
     5881 tcgtccagaa ccggaatacg ggacaggatc tctacgcctt cctgtgccag ctcctgcttg
     5941 aatgcctctg cggtcttctg gtcgcagccg ctgatcatgc agccgttttc tctggtgttc
     6001 agcagttcta cgccgacttc attggcaatc ttggcaaata cgccgctgcc gcagggagaa
     6061 gtccgatagc agcgtgctgc aaagaagtcg tcgatcggtg caacaaagtc tgcgccctct
     6121 tctgccggct cccagctctg agattccaca gtatcattct gctttacctc gtcgatcaga
     6181 tcgcccagga ctgcgcttgc ggtcggcagc tttccggcac cacgaccata gaacatggtc
     6241 ttgtccacgc cgtcgcccca taccataact gcattgaata cgccgtttac atgggaaatc
     6301 aggctctcgt tcatcactac cataggcata accgtaggca ggatctttcc gttttccaga
     6361 cgcttgactg cgccgatcag cttgactgcg ccgccgaaat tctgtgccag ctgtacatct
     6421 tccagggtga tgttgcggat acccttggtg tatacctgat ccggataaac gtgcttgccg
     6481 aataccagcg aggacagaat gcagatcttc cggcaggcat cgccgccgtc gatatccgca
     6541 gaaggatcac gctctgcata acccagctgc tgtgccaatg ccagcgcatc gtcaaagcgc
     6601 attttgtcgc agatcatctt ttccagaatg aagttggtcg taccgttcag aatgccggct
     6661 actgcggaaa tgtcattggc agccaggcag gtgtgcagcg gacggatgat cgggatagcg
     6721 ccgccgacgc ttgcctcaaa gaagaagttc acgtgatgcg cctttgccac ctggagcagg
     6781 atgtcgcctt tttctgcaac cagctgcttg ttggaagtgg caacgctctt gcctgcctcc
     6841 agacagcggc gtacaaagtc gaatgccggg ttgacaccac ccatgcactc tgccacatag
     6901 ccgacctccg ggtcgtccag aatcacctgg atatcgtgaa ctacacggtc accgtaggga
     6961 tcgtccggga actcccgcag atccagaata tagcctactt ccagctctgt gccggtgttc
     7021 ttttcgatct tcttctggtt gcggtagaac agttctacca caccagaacc aactacgcca
     7081 taacccaata ctgcaatctt tttcattata atactttcct ccatattcag cagcaccaca
     7141 cgcaggatgc cgcatcattc tcattgcctt tggaaacacc gaatcaagcc agctgttgtc
     7201 ctgggtgttt cttagaacct gtcttcacaa gaaatatatg tggattttcg agcataattt
     7261 tgttgcagac aagaaaaaaa tacgcaggcg ggctgtcgcc cggcaagtat tttttccgca
     7321 gtatgcagca aaatttgccg aaaagacacg tgtatacgct tgtgaagaca ggttcttatt
     7381 ttccggacag cagccgcact tccaccacgc cgtgcagatc agagatcaca cgtgtgatct
     7441 cgatgggatt tgccatgccc tctcccatct tgagagaaag ggacaccagc gccacaccgt
     7501 ctaccgggat attctgattc accgtaagga tgttggcgtg catactatat aatgtagtca
     7561 gcacattgga aagcacgcct gcctggtcac ggagcgtgac ggaaaagttg acgatctgct
     7621 gtgtcatctt gtcatcatag gcaaagacac agtcccgata cttatagaaa gcgctgcgtg
     7681 agatccccat gcgcttgcac gccgaggaaa agctgcgctc tgttttatct gccacaagct
     7741 tttttacttc cagcactttt ccgaaaattt ccggcagtac cgcagtatct actacaatgt
     7801 atcgtgtccg tttgtccata tcctgtctgc acagcagcgt ctgtcaaaac ctactgcgtt
     7861 tttcctcctc ttcagaatat ccgggcttgt ctgccgcttt gcagcaaata gtccgtgtac
     7921 aaaaaacaat tgtacttata tcaactatat aactatacca tgattttttc tgtttgtcaa
     7981 gggcattcta ctgttttttc tacgaaaaca acaagaagtt gttttcacag gtcagcccgt
     8041 gtctcttttc cggtggaaac cgtctccggg tttgtttcct tttggtatgc agcgcaccga
     8101 tgacgcatcc ggacgaaaac cggaataagt ttttttcaaa aaaagccgtt tttttctttt
     8161 tttccataga aaaaaacttt gtttcgtttg aaaagcaaga caattgcaga ttcgggggaa
     8221 aatcacttga caaatttcaa aaattcgagt atgataataa tgtcaattgg aaaaagcaca
     8281 gcagttccgc tgaatttcct gtgctgtgac gaattgtgac aaggagagca gacttgaaga
     8341 acagacagca gcagatgaaa ccaaatacac accgattcaa acgggtgtgc tgccgctgga
     8401 tgccgacagg catttcttct gaccgctcgg aaatgatagg tgaaacaaag gcgtttccat
     8461 gatgaaaccg gtgcagcttg caataagctg tacgcaaacg gtgaaccatg aaaatgcttt
     8521 ttatttttag gaggtaatta tcatgtccaa aaatgttgct gagtactttg catgcgacgt
     8581 cttcaatgat gaagtcatga aggcaagact cccgaagccg gtctataagg caatgaccaa
     8641 aacccgtaag atgggtactc ccctggatcc cacttatgcc gatgttgtgg caaatgccat
     8701 taaggattgg gcgatcgaac acggtgcgac ccactatacc cactggttcc agccgatgac
     8761 aggttctacc gcagaaaagc acgactcttt catcagcccg actgacaacg gcatggtaat
     8821 tatgaacttt tccggcaaag agctggtcaa gggcgaacca gatgcctctt ctttcccgtc
     8881 cggcggtctg cgtgagacct gctctgcaag aggctatacc gcatgggatc cgacttccta
     8941 ctgctttgtg aaagaaggct ccctgtacat tccgactgtt ttcgtttcct ataccggtga
     9001 ggcactggat aagaaaacac cgctgctgcg ttccatggac gcactgagcc agcaggctgt
     9061 ccgcatcctg cgcctgttcg gcaacaccac cgcaaccaag gtcacttcta ccgttggtcc
     9121 ggagcaggaa tacttcctca tcgacaagaa gatgtacgac aagcgtgaag acctgatcat
     9181 gaccggccgt accctgttcg gtgcaatgcc gccgaagggt caggagctgg atgaccagta
     9241 cttcgcaaat ctgaagcccc gtgttgcagc ttatatggct gacctggacg aggcgctgtg
     9301 gaaggtcggc gtattcgcta ccaccaagca caacgaagtt gcaccggcac aacacgaaat
     9361 ggcaccggta ttctccacca ccaacatttc caccgaccag aacgagctgg caatggaagt
     9421 gatggaaaag gtcgctctga aacacggtct ggtttgcctg ctgcacgaaa agccgttcga
     9481 cggcgtgaac ggttccggta agcacaacaa ctggtccatg gctaccaacg atggtcagaa
     9541 cctgctggat ccgggcagca caccgatgga aaatatgcag ttcctcacat tcctgtgcgc
     9601 aatcatcaag ggtattgatg aatatgcaga cctgctccgc atcagcgcat cttctgccgg
     9661 aaatgaccac cgtctgggtg caaacgaggc accgccggca atcatctcca tctttatggg
     9721 cgaagaactc cagggcgttc tggacgcact ggaggcaggc acttcctaca agagcgaatc
     9781 caaggtattc gagatcggcg tagatgctct gccggacttc ccgaaggatt ccaccgaccg
     9841 gaaccgtact tctccgtttg cattcaccgg caaccgtttc gagttccgta tggtcggctc
     9901 ttccgacaac atcggctgcc cgaactctct gctgaatgcg atcgtagccg gcgagctgtc
     9961 tgacatcgcc gacaagatgg aaggcgttcc ggctgacaag tttgatgacg agctgaagtc
    10021 cctgctggtt tccatcatca aggatcacaa gcgtatcatc ttcaacggca acggctactc
    10081 cgacgagtgg gtcgctgagg ctgaaaagcg tggtctgccg aacttcagat ccaccgttga
    10141 ctgtgttccg cactatgcag acccgaagaa cattgctctg ctggaaaaat tcggcgtact
    10201 gtctgaaaag gaaatcaagg ctcgtatgga agtcctgctg gaaaactatg caaagaccat
    10261 caacatcgag gctctgacca tgatcgacat ggcaaagaaa aagtatatcc caacctgcat
    10321 cgcatacacc aaggaactgt gcgacgctgt ggctgtgaag aagtccctgg agatctgccc
    10381 gtgcgtcgaa aaggaactgg cagaaaaggt aagcggactc accaaggatg cttataccgc
    10441 aaccaacaag ctggaagagg ctgttgccgc agctgctgcc aaggattcct gtgttctgga
    10501 tatggcaaag tcctatcggg aagaagttat tccggtgatg gatgcactcc gtgccgcagt
    10561 agacggtctg gaagtaattc tcccgtcctg caagtggccg gtaccggcat acagcgacat
    10621 tatcttcaat gtctgagatc tgccgtaaaa tagcgcaaga atgagaagaa tggattacct
    10681 cccattcttt tgaaagaccg tctgtgtttt cgcaggaaag cacaggcggt ctttttgtgc
    10741 atcattaaaa caaattgttt tgatttttcc cttgacaact gcactgccct atgctatact
    10801 ataatcataa accgttgcat gatgtataca acagttaaaa cagttatttc atgggagctg
    10861 atctacaatg ctaacaaaac ataacaacat aaaacgtatt ctcgcagtga tcctggcagt
    10921 tctggcgctg gtcggcggtc tctacggcat atgccgggca gtgagaaccg ccagagccta
    10981 cgacgtggca ccgctgacag atgcacagct gacagaccta gacttaacgg gcatcaccca
    11041 tctgatgatc gtagcgcacc cggacgatga aacactctgg ggcggagcgc atattgcaga
    11101 cggcggctat ctggtggtct gcatcaccaa cggcaacaat cccacacgtt ctgcggagtt
    11161 tcagtctgtg atgcaggcat ccggaaatgt ggggctgatc ctgtcctatc cggacaaggt
    11221 tgccggaaag cgggacaact ggaatcatgt ccgcacccag atccggacag acctggaaaa
    11281 ggtcatgacc tatcagccct gggaggacat tgtgacacac aacgccaagg gcgaatacgg
    11341 acacatccac cacaaaatga cacaccagat cgtcaccgca ctgtacgatg ccgaccagct
    11401 gcaaatgccg ctctatgtct ttggcaagta ctaccgtgcc gcagcgctcc cggatgtcaa
    11461 ggacacgctg acgccggttt ccgacgaggg actggagaaa aaggagaaac tgttacagct
    11521 gtacacttcc caggcacata cgatccaggg actttctcat atgaacccct atgagatgtg
    11581 gacgcagacc cgtggaggtg cggcagatga tacagcggcg taatactgcg acgaaacggc
    11641 agacgctgct gcactgtggc gcactggcgg cgattctggg agttgtggtc gggctgatct
    11701 ttctggtgca tcccggctgt gtctacgggg cgcagactga ctggagcaac cagcacttcg
    11761 ccattccgga atatttccgc acccggtttt atgccaccgg aaatctattt cccaactttg
    11821 ccatgcagct gggcagcgga cagaacattt tcaatttcgc ctattacggc ttatacagcc
    11881 cggtgatcct gctttcgttc gccttcccct ggatgaccat ggcgacctat ttgcagatct
    11941 ccaacattct gctgacgctg atcggtacac ttctgtgcta cttctggctg cggaaatggt
    12001 ttgttccaaa tctggcattc ttcctggcgc tcctctatct gacagcggca ccggtgattt
    12061 ttcacggtca tcatcacatt atgtttgtga actatctgcc ctttttattc tgggcgctgc
    12121 tctcggtaca tcacgctctg gaaaaacggt tctctctaca tctgatcgcc gcatcggtct
    12181 gcattcttct caccagtttc tttttcagcg tcggcgcatt ttttgccatt gtcatttacg
    12241 ccgtctttct gacgctgcac cggcatcacg tacaccaggt gcgtcagatg ctccgcacgc
    12301 tttgcattat cggactgcat ctgctgctca gtgctgtgat tgcagccttt ctgctgctgc
    12361 cggttgcctg gacactgctc cacggcagag acgcctccgg cagttcacag tctctctgga
    12421 gcatcctgct cccgggtgtc cacctgcgct atctgaccta cagtcccttt tccatcggca
    12481 tgacttcgtg cagcgtcctt gcagtctgcg ccatgctctg ctttcccaga agatcgaccc
    12541 ggtttctcgc tggcgtattc ggcgtgattc tagtctgccc tctgctgctg tatctgatga
    12601 acggcaccat gtatctcgac ccgaaagcgt acattcccct gctgcccctg ctgctgctcc
    12661 tgtgcggttt tttctggcgg cagctccgtt cccgtcagat actgctgaaa cccacgctgc
    12721 tgctgtttgc atcggcactg gtgggtatcc tgaccaacag cggcacggct gtggagcaga
    12781 tcgcagccat tctggacggc atctgtatgc tgctcgcctt tgtgctgtat ctgcaccggc
    12841 aaagcacccg gaaacccggc aagtcgctca agctcctgct gatgcctacg ctggcgttca
    12901 gcattgccac ctgtctggcg gtcaatctgg gcgacaccta catgaccagg aaagacgtgg
    12961 acattgttta ctcgaatgag atccagacgc tgaccgacca ggttgccgca gacgatgact
    13021 cgttctatcg catctccaac gaataccacg tcggagacac ggtaaaccgc atctggggaa
    13081 aggactacta ccgcagttcc gtctattcct ccattcacaa cgcatcgttc tccgatttct
    13141 acttccacca ggcatacaac gaaaacggca tccggaatgc ggcaatgatt gtccagtcca
    13201 agaatcccat cttcggcatt ctgatgggcg aaaagtacct catcagccag aaacccattc
    13261 accggtacgg tgtacagcag atcggacagg aaggcgatta cctgctctat gaaaacaccc
    13321 tggcgtatcc catcggtttc gccacatcca acgtcattac cacccagcag ctgaaacaat
    13381 tccactaccc cagccggctg gaggcactgc tggaaaatgc ggtggtggat gaggacgacc
    13441 tgccggacaa tgcctcttac caggcacatc tgccggacag catccagaaa atttcaccga
    13501 aatatacggc gacctcatgc agcaataacc agatcaccca gctggaggac ggcaagtttt
    13561 acgtccacag caagaaggac ttttctgtta cggtcacgct gcaggaaccg atcgacgcac
    13621 tcctcctgct gaaattccac gtggacaatc atctgggaaa cggcacgact accggggacg
    13681 ttgcaatcac catcaacggc atccgcaaca agctgaccga tccgcagtgg aaatatcaga
    13741 atcagaacaa caacttccag tacaccatct cttcggacga gccgatccag aagttgaaac
    13801 tgaaattttc cgccggagac tatgtgatct cgcaaagcgc catgtacacc atgcagtatg
    13861 acacactgca ccatgcggct gaacagctca gcccctggga gctgcgccat gataatctgg
    13921 gcgacgacac catggagggc gacatccagg tgaaagagga cggttggttt gtgctggctg
    13981 tcccctatga caccggattc cacgtcacca tcgacggcaa ggacactgca tactaccgca
    14041 cggacaagga cttcctgggc tgtcccatct ccgccggcag ccacagcatc cgcatcacct
    14101 atgcggcacc gctggcaaag cagggacgct ggtgttctgc cgcaggggtc ggcattacag
    14161 tactgctgtt cctgggcaac agcctgctcc ggcatcacag acgaaagaaa cgcagcagct
    14221 tataaagcaa atcatttatc acgcaaaccg cattttcaga ggttctggaa atgcggtttt
    14281 tttatcggtt gatctcacgc ttgtccgtcc ggttgagaag atagtccacc gagacgtcat
    14341 aaaagtctgc caggcgcacc aggacttcgg tgggaatatc gtggccgccg gattcataat
    14401 agctataggt gcgctggctg ataaacaagt tcttcgccat gtcagtttgc gtcatgtcca
    14461 tgtcctcccg taaattgcgg attcgaatat actgcataaa atatcaccct ttcaagatta
    14521 tggttttatt ttatgctaga acaaaacgtt ctattgactt ttagaacaaa acgtgctata
    14581 atatagctgt cacatttcag aaatctgtga cgaaaacaaa tctaaggagg acgttatgcg
    14641 taaaaaatgg attgcagggt tgctggcaat ttcgatgatt gcggcaatgg gtggctgttc
    14701 cggtgccgga accagcagtt cttcaaaaaa tgtagaaacc tcagcagcgg aaagcaacat
    14761 tgaggaaagc agcgaagaag aatccagcca aagtgaaact tctaccgcag agagcagcat
    14821 tgaagagttg gaagaaaaca gcgctccgga aacaaacgat ggtattcgac cggatatcaa
    14881 agaagcaatt gatagctatg aatcttttat gaatcaatac tgtgatttca tgaacagtta
    14941 tgatgcaact gatttgtcca tgctttccga atactccgag ttaatgtcag aatatgtaac
    15001 aacaactgca aaattcgaga agatacaaga tgaagatctg aatgatgcag aactggcata
    15061 ttacttggaa gttagcaatc gtgtttctca aaatctcctg gcagtttctt ctgcacagta
    15121 accaattcaa aaagaaaaga ggatgtttcg aatgaacgaa aataaccagg tggcaacacc
    15181 aaccacacag ccgcaagcca atgcaccgaa aaccaagttc tgtaaacact gcggcggaaa
    15241 aattccggaa gatgcagtca tctgcacatt atgcggcaga caagtggaag atatcaacaa
    15301 aggtgccaac gctgcacagc cgcagattat cattaacaac gacaatatca ataccaacac
    15361 aaatacaaac aatgttaatg caggcggcag aggaaaagaa aagaataagt gggtcgcact
    15421 tcttctctgc tttttcctgg gctttgtcgg cggacacaag ttttacgaag gaaaagtcgg
    15481 catgggcata ctgtacctct ttaccgcagg actttgcggc atcggtgtac tgattgactt
    15541 tatcactttg ttgtttaagc cgaatccgta ctacgtgtaa gcttatatac agcttaaaaa
    15601 tacaaagcaa aaagcgctgt gcagcggaaa tgctgcacag cgctttttca atctatgaaa
    15661 tatttctttc ttactgttct gcctgctgtt tccggcgagc ctccagctct tccagcgcat
    15721 ccttgcggga caggctgatc ttgccgtcct tgatgtccat gaccttgacg atcacagcat
    15781 cgcccacaga gaccacgtcc tcgacacgtt ctacacgatc cttgtccagc ttggaaatat
    15841 gcaccagacc gtcacgtccc ggagcgatct ccacgaacgc accaaacggc ttgatggaaa
    15901 ctaccttgcc cttatagatc gaaccaacct ccgggtcaat ggcgatggtc tcgatcatct
    15961 ggtatgcctt gcggcagcca tccatggaaa tgccgctgat gaatacgttt ccgtcctcgt
    16021 ttacgtcgat ctttacgccg cattccgcag acatcttctg aatgacctta ccgccctgtc
    16081 cgatgacatc ccgaatcttg tcggtcggga tcttgatctg gagcattttc ggcgcatatt
    16141 cgcctacggt ctctctcggt gccgggattg ccttcagcat gatctcatcc agaatgtaca
    16201 gacgagcctt gcgtgtcttt tcgaatgcct ctgcgatgat cgccggtgtc agaccgtcta
    16261 ccttgatgtc cacctggatg gcagtgatac ccttcttggt accgccaacc ttaaagtcca
    16321 tatcgccgta gaaatcctcc agaccctgga tgtctaccat agtcttgaac tcgtcgctgt
    16381 tgggcagtgt aatcagtccg caggaaatac cggcaaccgg tgcatgaatc ggcacaccgg
    16441 catccatcag cgccaatgtc gagccgcaga tcgatgcctg agaggtagag ccgttggagg
    16501 acagtacctc agagaccagg cgcattgcat acgggaattc ttccaccgtg ggcaacaccg
    16561 gaatcagcgc acgctctgcc agtgcgccgt gaccgatctc acggcgtccc ggtcc
//
LOCUS       DAWENO010000031        16489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1469_length_16489_cov_12.034015, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000031 DAWENO010000000
VERSION     DAWENO010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16489
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1469_length_16489_cov_12.034015"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1650..1659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2944..2953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4846..4855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6440..6449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3603 a         4778 c         4495 g         3573 t
ORIGIN      
        1 gggtgcgcac atccgtatcc ggcggcgcca gcagaatgac ctgtacagat gccgtaaagg
       61 gatagtacag gaattcgccg ctgagaaagg gcgactgcat ctccgtcccg gaaattccca
      121 cggtgaggaa ccagtccggg cgcttgtcga agggtgtcgg atcataggca tcgtagatct
      181 gccactccgc ccggtccgcc agcgccgctt tcaccttttt caaaagctga tccatgagca
      241 ttgcgctcac ctcctttctt atgccactgc cagaaacaga aacgatttct cttccagcag
      301 ggcgtgacac aggttctcgt atgcctgcac cagcagctcg gcaaagcgca gtttctggct
      361 gccgtcggtg ttctgtgcca ccgtcccggc aaaggtgcag gcggcacggt ctcttgcggc
      421 ggtgatctgg gtatagcgca gaaatcccac cgccgccgcc agccagttca gccgggcatc
      481 cgcagcgtcc gcatcgggac gcagcgcctg ctccacctcc agcacagcag tttccagcac
      541 tgccccgtag gtctcaccca gcgtgtcgtc tccggcaaac agcccgaaca gacgtgcaat
      601 ttgtgcttgt tccatttgac ttcacctcct ctcacggttc cagttctgcc tcgatctgcg
      661 ccgcctgggc gttccacagt ctcgcctgtg catactccgt ctcgtcctgg agattgatgt
      721 tgctccactc caccgacacg tcagcctgcg acccggcgag ccgcagaaac gccgttccca
      781 cccggcgcag caccgggtct aacaggcggc ggtaatattc cagctctgag gtgaggatat
      841 ccgcctgctg actgctcatg cgctctgtgg atgaccagtt cagccccagc agaaacggcg
      901 gaatggacag cttggcgatc agctgttcca acagctggcg caccggcact tccgtgtcca
      961 gcagggcatt gtcagcgccg atgacccgga tgtccacatc tccggcgcag ataaagtccc
     1021 ggatctctcc ggctgccccg gcacgcatcc cctcgctcca ctccttggcg atctgccttg
     1081 cacgctctcc ggcataggct ctctcgttgg ggtcgttgga cggcttatag gtcacggcat
     1141 agcgcacatt tcccatccgg tcgtagttct gcccgatgca ggcatagatg cgcagcaaaa
     1201 tgtgacttag cgccggcagc ccacgcagaa cggaaacgcc gtacacaccg cccggcggcg
     1261 gattcagcgc cgtaaacaaa attcgttccg gtctggcgat gcggatgctg tccgactcct
     1321 gctcgccacg cagccagtac tgccgttcca ggggatggct gcccggtcgg atctgcacca
     1381 gctctgccgg cacagtctgc aaaccgccca gatagccggt gcggttgtcg atgagcattt
     1441 cccccagggc attgccgtag gtgagcaggc tgtccagata ggcgtctgca aaggtctgca
     1501 gcgactgccc ggtcagcccc accggaacgc ttcggacaaa ctcgtccagc tgttcctgca
     1561 tccggctgtc tccggcgacc agctgaaagc tgccggtgag ccggacgatc tttccaatgg
     1621 cggcgtcgat caccgggacg gcacggcgcn nnnnnnnnnc cggcatacag cgccggttca
     1681 caggggtcct gcgtctccgg cagcgcatac tggctttctg cccgtgcggc gctctgcaaa
     1741 atgctgccgc ttacgggcgg tttctttttc ctcggaaata atcccataca catttacctt
     1801 cttcctgcgg aaaccgcata aaatccgtct gccggattcc ggcagacaac ggtgcggacg
     1861 aaatagcgca tatcgtccat ggcgtggtcg tgttccttgc gtggggcatc gccgtgtgcc
     1921 gaatcgttcc agcagtactg ggtgaattca cgccggatat cccggcagga ttcgcagaac
     1981 ctcagcttgt cctgcatcag caggcggctc acctgctgga tgccggcgtt tacgtcgttg
     2041 tccgcccgga gtacccgaaa ccgaccgtga ctgtggatgc aggcgataaa gcttgctgcg
     2101 gaagggtcta cgatcacggt ctctacggca tacccggcgg caagctgttc caatgcggca
     2161 tagtgttcct catcggtgcg ccgctcgccc tcggctcttg catcataata gtattcctcc
     2221 agccggtacc agaccccgtc acagtgtccc cacagcccga aggaggacgg gttcactgtg
     2281 ccgtagtcac aggagatcca gaaccggtca cacgccggtg cttttgccac cacatggcgt
     2341 tccggcgcaa acatgggata taccagcccg tctgccgccg tccattttcc caggacaaag
     2401 cggtcataga acgcaccact gtacatccgt tcataccgct gccgcacacg ccgggaaagg
     2461 gaggggttgt ccgccatggt gaagtggaca tacagggcgt gcttttctcc ggtctttcgg
     2521 atccattccc gatagaacca gtgtccgggg tggtcggggt tgcagttgaa ccacagccgt
     2581 gagccagtca cgctgcaccg tgccagcgcc tgttccacaa aggaacgggg catcagcgcc
     2641 acctcatcca gaaacacgcc gcagagcgtc atgccctgga tcagcgctgc ggagctttcg
     2701 tccctgccgc cgaagagata aaaccggttc gtcctgccgc acagggtgat gtccacatag
     2761 ttccggctca cccgttccag acaggtgaac cccaggtttc ccagcagccc cagcagcggc
     2821 gtcatcacgt tgcgtttcag cgccgtgatg gtcttgccgc acaaggcgaa gctgccgccc
     2881 tggaaactgg tcatcgccca gctgataaac ccgatggaca tggaaagcgt ctttcccgag
     2941 cggnnnnnnn nnngcggact gccccgtcac agatgatggc gtcatagtcc cgatagggct
     3001 tgcggctcca ccaggacatg gcgagcttct gcttgggaga aaaggtctta aactccatcg
     3061 ccgttcaccg cctctccgcc ggaaagcgcc gcaagcagtg tctgcgccgc cgccttgctg
     3121 tccgcatcgc cgctgtactc atagagcgac gccagcgcct tcagccggtc aaaaaagtgg
     3181 atctccatgc cgccgctctt gtcccgcttg atcgaggaca ccggaaacag atccagctgt
     3241 gccacctctg ccgccgtggg cagctcttcg cccagcagga gccgcaccgc atcattggtg
     3301 cgtccgaacg ccaggcgctc cagtccggca cgcaccatcg ccatggggtc atcatgaaga
     3361 acacttcgat agtttgtcac catgcgccga tagcactgct gccgcaggat cgctgcgccg
     3421 tccgctgctg ctgtctccgg cggatagccg gcacacctcg ccgcctctgc cacatccccc
     3481 agtctggcgt agtgacagca gaacaggctg cgtcttttgg tctctgctga atttgacata
     3541 tctttccatt tcccctttcg gttttctgcg acaaggatcg catctcattg acgacccctc
     3601 tcactaatac ccccgcagag ggcaaaagtt gcacgccggc gtgcaactat gaactcgaat
     3661 ttacacgata tcttatgatt atttacattt agaacggctt ttaccacgtt ttggcttgac
     3721 agataggttc cgccgtgcta taatatatgc gtaatcaata ttatacgtcg tataatattg
     3781 caggagtaaa aaacaagagc cgatgcaggc atcgactcaa ggcgcttttg tactatttga
     3841 aaaggcgagt caactcctcc gcccttcggg cacctcctct gggagaggag gctaaattgg
     3901 ctcctctcat agaggagctg gcagccgcag gctgactgag gagtcaatgt tcacccctgc
     3961 gacaaccaaa cacatccgcc cctgcaaaat cccctttccc aaaaacgact cctctctgaa
     4021 agaaggtgaa accattgtct gcattttcca ttggagcaac gctgctggat gctatggtgc
     4081 tggcgctgct gacgaaggag gatacctacg gctatcagat caccaaggac atccaggaac
     4141 actttgccgt atccgaatcc accttgtatc ccgtgctgcg gcggctgcaa aaaaacggca
     4201 gtctcaccac ctatgaccga ccgtatcaag gcagaaaccg ccgctattac cgcatcaccg
     4261 tctccggcag agcgctgctg gatcagtacc ggaaagactg gctcacctat aagcaacagg
     4321 tggacttttt cctggacgag aaataattgt agtgcgatgt aaattctaaa actttataga
     4381 ttaggctccc ttgaaaaggg agctggcagc cgtaggctga ctgagggatt tgactgtgaa
     4441 accagcaatt caagaaaaaa tttcaccaga atccctccac cagcttcgct ggttcccctt
     4501 ccttttcaag ggaggcttat tcctatcgtt ttaactgtta catcgtagta aggggcgatg
     4561 caaacaccgc cccgcataca cccccacaat ccctcatcat ccaaaaagca atcttcaaaa
     4621 aggagcgata ttcctatgac accacaagca tactgcgccc gtctggaaca ggcgctttcc
     4681 tccatgccgc cggcagagcg gcaggagatc atccgctatt atctggaatt cctggaggac
     4741 gccagcgagg acgaacgcag cgccctcggc acgccggaag aactggcgca gcgcattctc
     4801 cgggaaaacg gcatctttcc gccggacacc gctgccgcac cgccgnnnnn nnnnncgctg
     4861 ctcaccacct ggtacagcct gctgcttacg gcgctgatcg tcctggcggt gattcccatt
     4921 gccctctgcg ccgcactgct gggcggactg ttcacctttg tcctggcttt gttccaggat
     4981 gtgccgtctg cgctgatgca gctgggcatg gggcttgcct gcggcggcgt cgctgccctg
     5041 gtcgcttatc ccatctggat cgcctgcaaa agcattgtaa aattcgccct gttcaccaca
     5101 aaaaagatgt ggcacttcgt cacgaaggga gacgataccc catgaaaaaa cgctgtattc
     5161 tgatcggcgc agtctatggg ctggtgctgc tgggctgggg catcaccggg actttttccc
     5221 ctgccgcccc gacccgttcg gagcagtcgg acaatgccgt acctgtagag accatccagc
     5281 tggaacagct ctgcatctgt ccgggaaaca ccttttccgt ccggacgagc cagccgcttt
     5341 ccgcatcccg gaatggctcg gtgctttccc tgcggcggca atggcagctg tttcagcgcc
     5401 acagcgaaaa cgatatcacc attaccctgc cggcggcgca gtttgcacag ctggagcagg
     5461 cagttaatac gaccaaaagg agtgaccaga aatgaaaatg aaaccttgga tcatcaccgg
     5521 tgtcagcctg ctctgtgccg gagcgatctg ctgcggcgca gccgtccatg ccggcgcact
     5581 ggatcagaaa cgctacatga aaatgctgga tctcaccgac tataccgaca ccccggacgg
     5641 tgctttttcc tccgtgctga tggatattga aagcgccgat gtgaccgtgc agaacggcag
     5701 tgcgctttcc gtcgccgcaa agaatgtccc ggaggacagc tacagcgtca ccgtcgaaaa
     5761 tgacactctg cacattcaga tgagttccaa caacgagccg tggtacagct tcacccgttt
     5821 cggattccac ccgttttccg ctccacgggc gaagatcaca gtgaccctgc cggatgcgga
     5881 ataccaggca gtggctgtgg caaacagttc cggagactgc accgtttccg gcatcagcgt
     5941 cagcaccctg tccctggatc tggattacgg cgactgctgg gtacagaatg tgacggcggc
     6001 gagtctgact gccgtcaaca aagccggaga actgacgatc tcggacagca gcgtcagcgg
     6061 cacggcatcc ctggtgctga attacggcga tctcacagcc aaaaacaccg aggtgcagaa
     6121 tctgtgcaag gtgaaaaacg atgccgggga catcaatctg accgattttt cctgcggcag
     6181 cagcgaaatg gaactggaat atggcgattt gaaactgaaa aaattcaccg aaaccgacaa
     6241 gaccaagtct agtgcattca ccctttccga tggtgatgtg cggtgcgaga ccagcacgct
     6301 ctggaacagc agcttcgaca tggatttcgg agacattcag accacagaca cggcgctgta
     6361 cggcaatact gccatttcca tggactacgg cgatgtgcgg ctgaatctcc acggcagcaa
     6421 cgccgactat aatgtgggcn nnnnnnnnna ttcctacgct gccggttcgc tgaacggcag
     6481 cagccccaac cagatcgcca ttaccggtga ccagacgctg atggattcca gcgttacgtt
     6541 tacggaatga gctgaaacgc aaaaaaccgc atcgcctccg gaaaatttcc cggaaagcga
     6601 tgcggttttc tatttgtatt cgtttctgct tacagaacca cgccgttctg ttccagcagc
     6661 gcctgtgccg ttgccacaga atccacaccc tcggcgttct ttaccggggc aaattccctg
     6721 gtgcggtcta cctcaaacgc cagacagctg tcctgcatat gcaccatatc cagcaccagc
     6781 gtctcgaact tgtagccgtt gggcgtgtcc ggctttacca gctcgccctg ttcgttcaga
     6841 tgtgcgatct tcttgtgtgc gatgtgcagc ggaaaatccg aacccagggt gtgcatcagc
     6901 gcatccaccc ggaacagata gttcaggatc acgccgaacc gataggcgag cgtgccgtcc
     6961 ggattgcggc gtgtgcgcat ttcctcggtc atctcgtagt attccacgat ggacggcttg
     7021 tcgtcctcca gacatagaac gcctacacgc tcgtccggtg ccgccttgct gactaccttt
     7081 ccgccggacg cacagcccga cgcaatggtc gcacccagga ataccgggtc agcgatctgc
     7141 tgcaatacgt tgtccacggc gaatacgttc agccactcga tgccgatctc gttgaccagc
     7201 tccagcagac cggcacgctg catggaagag aaccagccgc cgttgccgtt gggcgagagg
     7261 acgatctgat ccggagcgct catgaagatc tttccgtttt cgtccactgc cggctcggtc
     7321 tcctgccgga agaaatgcac attgtcggcg ctgtagccga aatagtcgtg ttccttcaga
     7381 aaagcacgtg tttctgcgtc attctgctcg ctggtcatga tgaaaagcgg cacatacgcc
     7441 tgcgcctcat cggtcacctt tttcagattg tggatcagac actcaaagat atacagatcc
     7501 cgtgtctttc ccatgttgta cattcccttg ggatggtcaa atcccagccg ggagccctga
     7561 ccgcctgcca gcagaacggc gcccactttt gcctcccgga tcgcccggag accgatctcc
     7621 cggtattctt ctgatttttc agcgatctgt tccagcgtcg ccgcacccag gggcgcaaag
     7681 ctgccacgct tttgtgcagc ggcatcccgg ttctgaaatt gcggacgctc cagctgcaag
     7741 ccttcgatct gttccagcag gtgtgcctgc tgttccggcg tcagctggtc gtaatagcgg
     7801 agcaggtgtt cctgtccatg ctccgtcaga tatgccagtg cctgttcgta tgtcatatgc
     7861 catatacctt tcttgaaaaa accggtgcgt taccctcagc gcctgtttcg catcccttgc
     7921 accgggcttg ttgttttctg gaaatgccgt aatgttccga tggcattgcc gtacaaatga
     7981 tagtaaccgt attatatcac aatcttcgga aaaaaacaag cctttttgta aaaaaacgta
     8041 cgatttcgga agaaaatgag gcatatgcga aatcgttccc acaaaatcct acataatttt
     8101 aacacaaaaa aatgtatgct taacgggaat acggcacaag ccccaggatc tcgaacgcct
     8161 gggagcgctg ccacaggtcg gcagtcacgt agatgtcaaa gccctcaccc ggctggcact
     8221 gcaattcctg gggcgagatc ttcggcagcc cgaacccggc cagcatattg gcgatcatgc
     8281 cgccgtgggt aatcatggcg cagtgcagca gatcttcccg catcatatcg gtgatgatct
     8341 cccggagcgc cttgaaaatg cgcagctgta cctccttgac gctctcaccc tgtggcggac
     8401 ggcagtccat gccgcctttg agccactcct tgaactccgg cttttccacc agttcgtcgg
     8461 cggactttcc ctcaaatgcg ccgaaatcca tctcgatcag attgtccaca gtctggatct
     8521 gcacgtccgg gaacaggatc tctgcggtct cggtacagcg ccgcagaggg ctgctgtaca
     8581 cccgtgccac attgggatat tggaactcgt ccatttttcc ggcaagctcg gctgcgccct
     8641 tgtcggaaag cggaaaatcg gttctgccga tgtaaatgcc cttgtcgttt gcggcagttc
     8701 tgccgtgccg gatcacgctg atgcgataac ctttcataaa aagagaaacc tcctgtgcaa
     8761 aattgttggg gaaaccagaa tctccgcaaa acctgccggt tctgttgcgg aataagtcat
     8821 aatttcgttg taaaataaac aattattttc cgtttacaaa agaaacgggt tgtattataa
     8881 tataggttga gggcaacaac gcacaaaaac ggcatccgtt ttttgtgcag tgttgccata
     8941 tctgcgccgt ttcggcggat gctgtttcgc cggaagcggc agcagatata cctttattat
     9001 atcatattcc ccgaagaaaa gaaagatgtt tttatgattc gctggaaaaa actttttgcc
     9061 gctctgcccc tgctgctgac ggcgtgcgca tctcccacag agcaggcgca gacggagacg
     9121 gtctgggcaa tggatacggc ttgtaccatc accctgcacg gcggcggcgt ttctgaaacc
     9181 gcatccctgc tgcgcaccct ggacgagacg ctggacaact acagcacaga cagcgccgtt
     9241 tcccggctga accaatcggg gacgctctcc ggcgaggagg acgtttcccg tctggtgcag
     9301 gagacggcgg cgctccagag ggactacggg gacagcgtgg atctgaccgt ggggcagctg
     9361 acccggctct ggggcatcac cacagatacg ccccacgtcc cgacccgggc ggagctggca
     9421 gaagttctgc ccaccatttc cccggaacac gtgtccgtcc aggcggacgg cacagttact
     9481 ctgacggacg gtgcccagct ggactgcggc gctgtgggaa agggctacag cctggatcgt
     9541 gtcaaggcgg cgctggacga gtccggcact gccgcatggg gtacggtctc tatgacctcg
     9601 tccattttgt gctacgggga aaagccggac ggggcagcgt ttcagatcca gatccgtgac
     9661 ccggacggag acggcacatt ggggacggtg tcaactgaca gttgtttctt gtccacctcc
     9721 ggcggatatg aacggtattt tattgcggat gacggcaaga aatactgcca catcctggac
     9781 ccgaaaacgg gaatgccggc ggaatccgat ctgaccacgg tgacggtgtt ctgcgacagc
     9841 ggactgaagt cggactttct ctccactctc atctggatgg agggcagtga gaacctgccg
     9901 gcgcatctga aagcgaagga ataccagatc gtcgcccagg acaccgccgg aacgctgtac
     9961 gtcagcgacg ggctggactt cacacccgac gcatagcaag ccgcatcacc cttacaagag
    10021 ccttatttat acaaggctcc cttgaaaagg gagctggcag ccgaaggctg actgagggat
    10081 tttgctgtga agtctgcaat tcaaggaaag gctttaccgg aatctttcca aatcattccc
    10141 aacgcctatc acagaaacca tttcatttct gtagatatat catgtaaagc aacccgaaaa
    10201 agcgaaagga gctgtattct atgaagaaac tgcatggtgt gcggctggca caccataaaa
    10261 acacagagaa ctgtgcctct gttcctctgc ccctgccgaa actgatgcgt attcccatga
    10321 gtatgcacat cggcgcaccc tgtacccctg cggtaaagcc gagggatacg gtgctggtgg
    10381 ggcagaagat cggggacacg gatgccttta tggcagcacc catccactcc ggcgtgtccg
    10441 gcacagtaaa ggcaatctcc acctaccgga tgtccaatgg cagaacctgc cccatggtgg
    10501 agatcgaaac ggacggacag cagaccgtct gtcccgacgt ctgtccgccc acggtgaccg
    10561 acaaggagag ctttctcaaa gcggtacggg agagcggtct ggtgggcatg ggcggcgcaa
    10621 gctttcccac ccatgtcaag ctgaatccca agcagaaggt agacaccctg gtcatcaacg
    10681 ctgccgaatg cgaaccgtac atcacttccg actaccgcca gatggtggag gcaccggatg
    10741 aagtgctgga cggcgttctc caggtgctgc actggctgga tattccaaag gcagtgatcg
    10801 gcatcgagac caacaagccg gaggcgatcc gcattctcac ggaaaaagcc aaggcacacc
    10861 cggaaatcca gatcttttcc ctgcccacta cctaccccca gggcgcagaa aaggtgctga
    10921 tctatcacag cctgggacgc accgtcatgg agggtcagct gccggcagac cagggcgtca
    10981 ttgtgatgaa cgtctcttct gtggcattcc tcagccgcta tctcaagacg ggtatgccta
    11041 tggtacagcg catggtgact gtagacggcg acgctgtggg aaagccgtgc aacgtgatcg
    11101 ttcccatggg aacaccggtg caggatgtgc tggatttcgc ccagtgtgac atggagcgtg
    11161 cgaaaaagct gctgtacggc ggcccgatga tgggcattgc catcccggac actgccgacc
    11221 cgattctgaa ggcaaacaac gccgttctgg cgctggaaaa cttcgtttcg ccggaaccgt
    11281 ctgcctgcat ccactgcggc agatgcgtcc acgcctgtcc cctgcggctt atgcccacgg
    11341 aaatcgccaa ggcgtaccgc cgggaggaca gcgaggcgct gggcaagctg aaagtgaccc
    11401 tgtgcatgaa ctgcggctgc tgcacctacg cctgtccggc aaaacgcccc gtggcagaga
    11461 ccaaccagct ggcaaaggcg ttctatatgt ccagtattaa aaaataaaaa ctcctcagtc
    11521 agcctgcggc tgccagctcc tctgggagag gagccaattt agcctcctct ttagaggtga
    11581 ttccccacgg ggtggggaaa tgtcaccgca ggtgacaaaa gggaccgctc agtacgaggt
    11641 gcccgaaggg cggaggagtc ttttgcctcc cttgaaaagg cgcccgtagg gtgtaccttg
    11701 tgctgaggtg gcatgaagtg cggagggata atccccctgt cagcttgcgc tgacatcccc
    11761 cttttcaagg gggacttcct tgaaaaagta tcactttact ttgtttcgag aagattttat
    11821 gcctcccttg aaaagggagg tgcccgaagg gcggagggat attctcctgt caccgtaggg
    11881 gagaatattg ttcgcccgag actcctcagt cagcctgcgg ctgccagctc ctctgagaga
    11941 ggagccaatt tagcctcctc tttagaggtg attccccacg gggtggggaa atgtcaccgc
    12001 aggtgacaaa agggaccgct cagtacgagg tgcccgaagg gcggaggagt caaccccttc
    12061 ttaaaaaaga aaccccatcc ccacccaaca cccgaaaaaa taaactcatg gaggatgata
    12121 aaccatgtca ttacttacaa tttctccgtc accccacgtg cgcagcagca tgaccacgca
    12181 gaagatcatg ctgctggtgg tgctggcact tgtgccggcg atcgtggcgg cgactgtggt
    12241 ctttggacca cgtgctttgt ttatggtgat atactgtgca gttatcagta tgttcaccga
    12301 actggtctgt cagcggctca tgcagaaaga agtgaccgtg acagacggca gcgctatgct
    12361 caccggcgtg ctgctggcgc tgaacctgcc ggtaaccctg cccctgtggg aggctgccat
    12421 cggctgcgtc tttgccgttg ctgtggtaaa acagctgttc ggcggtctgg gctgcaactt
    12481 tgcgaaccct gccatcaccg gacgtgtgtt cctgctgctg tccttcggcg gcgatatgac
    12541 cacctgggtc aagccgttct attatcaggg caataacttt atcgtcaagc tgctcatgga
    12601 taaccccaga atcccggacg gcgtcaccgg cgcaaccccg cttgcatccg gaaacgcaaa
    12661 cctgctggat ctgttcctgg gcaacgtggg cggctctctt ggtgagacct cggcgctggc
    12721 actgctcatc ggcggcattt tcctgctggt catgggcatt atctcctggc atacccctgt
    12781 ggcatacctg ggcggtctgg ctgtgctgga actgttctat accattgcca ccggcggcga
    12841 aataaccgat gtgctgtacg ccctgctcag cggcggcgtt atcctgggcg cattcttcat
    12901 ggcgaccgac tatgtgacaa cgcccatcac cggaaccgga aagctgatct tcggtctggg
    12961 ctgtgcggtg ctgaccttcg tcatccggga atttgccaat atgccggagg gatgctcgtt
    13021 ctccatcctg ctgatgaacc tgctgacccc gtatattgac aagctgacta tgacccatcc
    13081 tttcggcgca aagcccgcag tgaaaaagga ggcgaaataa catggcagaa cagaaaaaat
    13141 ccagtgcaaa ggcaatgatt ttgccgccgc tggtgctggc agtcatctgc gctgtctgct
    13201 gtgccttcct ggcgctggca aacatggtga ccaaggataa gatcgctgcc gccgaggcag
    13261 acgccatcca gtccagctta cagcagctgc cggatgccgg aacctttgaa gagatcaccg
    13321 attttacccc ggagaacaac gagaaggcga ctgccaccgg actgtaccag gatgaaaagg
    13381 gacagtttgc cgtgctggtc accgcagacg gctataacaa aggcggtctc caggttgtga
    13441 tcggcgtgga caaagacggc gctgtcaccg gcgtgtcttt cgtgaccgtt tccgaaacac
    13501 cgggtctggg taccaaggtg cagtccaatc cggaactgct ggtggataac ctggtgggac
    13561 tgtcggacga aacggtcgta aacggcgtgg ataatatcac cggtgcgact tattcttcca
    13621 agggcatgaa ggcagccgta aactgcgcac ttgccacagt cagcgcaaat caggaggtga
    13681 ccggcgcatg aactacggaa aggaatttac aaagggtatt ctgaaagaaa acccgacgct
    13741 ggtgggactg ctgggtatgt gtcccacact ggcagtcaca acggctgcgt ttaacggcat
    13801 cggcatgggc ttgtccacca tgttcgtgct gatctgctcc aatatcgtca tttccctgct
    13861 gcgaaaggtg atcccggaac aggtcaagct cccctgctat atcgtcatca ttgcatcttt
    13921 cgtgacagtc atccagttcc tggtgcatgg ttttgtcccg gcgctgtata ccagcctggg
    13981 cgcattcctg gaactgatta ctgtaaactg catcattctg ggacgggcag agatgttcgc
    14041 ctcgaaaaat aacgtgcttg cctcggcgct ggacggcgcc ggcatgggtc tgggctttac
    14101 cctggcgctg ttcctcatgg gcagcgtccg tgagatcctg ggcagcggca cctggttcgg
    14161 actgaccatc ccgggcatgg tgggacacac catgagcctg tttgtcatgc cggcaggcgg
    14221 ctttctggtg ctgggctttg tgatcgccgt ggtctgcgcc ctgtccaaaa agcagccgcc
    14281 gaagaaaatc ggctgtgcag gatgtcccaa tgcggcatcc tgcggcgcaa agtgtgagga
    14341 ggctgacgca tgagtactta cattgccatt atcattgctg ccgtactgac caataacttc
    14401 gtcctgtcga agttctacgg ctgctgcccg ttcctgggcg tatcgaaaaa gctggactcc
    14461 agcgtgggca tgggtgccgc cgtggttttc gtcatgttgt gcgcatccct gtgtacctat
    14521 cccatctact atttcattct gaaacccctg gagctgacct acctccggac agtggcattt
    14581 atcctggtca ttgccgtatt tgtacagctg ctggaaatgg tcatcaaaaa gctgatgccg
    14641 ccgctgtata agaccctggg cgtttatctg ccccttatca ccaccaactg cgctgtgctg
    14701 ggcgttacca tcatgaacat tgatgagagc tatagctttg ggcagtctct ggtgaatgcc
    14761 gtgttctccg gcgtgggctt tgccctggca atggtgatct tttccggtgt ccgttcccgt
    14821 gtggatgatg cggacagcga gaccccgaag atgttccgtg gcgttccggc aacgctcatt
    14881 gcggcagcga tcgtgtccat gagctttctg ggcttcggcg gactgggaca gtaaggagga
    14941 tgtgccatgg aatttgtcta tcccattttg atttttgttg tcatgggcat cgtgttcggt
    15001 gtcctgctgg tggtgatctc caaggtattt gccgttgaga ccgatgagcg tgccgtcaag
    15061 atcaccgagg cgctacccgg cgcaaactgc ggtgcctgcg gctatgccgg ctgtgcagac
    15121 tatgccgatg ccatcgtaaa caagggcgct cccatgaacg cctgtctccc cggcggcgcc
    15181 aatgctgcgg cagccatcgg cgggatcatg ggcgtttcgg tgtctgcatc cgagcggatg
    15241 atcccggtgc tgcactgcaa cggcaactgc gacgccacca accggaagtt cacctttgac
    15301 ggcgtgcagt cctgtacggc agcaaagcgg ttttacggcg gcacaggcgt ctgtgcctat
    15361 ggatgtcttg gactggggga ttgcgtttcc gtctgtgaaa acgatgtcat ctccatcaag
    15421 aacggcattg ccacattctg cacggaaaag tgcgtctcct gcaataagtg cgccaaggtc
    15481 tgtcccaacg gactgatcga gctgcgcccg gagaagaaaa aggtggacgt gcgctgttcc
    15541 tcccggaaca cgggaaagac ggcgatgcag tcctgcaaga attcctgcat cggctgcaaa
    15601 aagtgcgaaa aggtttgcaa gtttgaggcg attatcgtgg agaatgctta tccggtcatc
    15661 gactatgata agtgtaagtc ctgcggtctc tgcgccaagg aatgccccag agggtgcatt
    15721 gaaaacctgc gtaagccgaa ggttgtcgct ccgaagccgg aaccggcaaa ggaaaagccg
    15781 gcggaaaagg ctgaggcaga ataaagaata tactgcgcaa aaaatccggt ctgcctcttg
    15841 acaaaccgga tggaaacggg tataatgaaa atagaaagag cataccagta gacggttgct
    15901 cccaatctaa gaatggcttt tagaaatagc cgccttatgt ggaagtgagg cggttatttc
    15961 tgtttgttct taaaatttaa gtaagcaagg tatgccttga atacatcaca aacaatgctg
    16021 atcagattga caataacatc aagttccatg cacatacact cccttctggg agcgtggatt
    16081 aaccgcctac cgttttcggt atgccctagg gtagttatag catattttgc cgaaaactgt
    16141 caagccaaaa agcacgcaaa aaatccggtc tgtcccttga caaaccggct ggaaacgggt
    16201 ataataaaga tagaaggagc ataccggcag acggttgctc ccatgtttaa gagattaaag
    16261 aaataatcgc ctaagttggt agctgagggc ggttatttct ttttgtgatg attgtccttg
    16321 ttatgaaaga tgccgtaaag gattcctgta tatgttgcaa ggaatgtcag aagtgcaaac
    16381 aaatctgacc atgtcacata atgttccatt cgatcacctc ccatcgtaca aaagtacatg
    16441 ggagctggaa atcaaaccgt ccaccgtttt cggtatgccc tagggtatt
//
LOCUS       DAWENO010000032        16396 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1476_length_16396_cov_12.059482, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000032 DAWENO010000000
VERSION     DAWENO010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16396
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_16396_cov_12.059482"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3181..3190
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10404..10413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13173..13182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15260..15269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4455 a         3928 c         3636 g         4337 t
ORIGIN      
        1 gcgaagttta gaggtttcgt tattcgtaca aatttctgtg gtattcggga cttgcgttgt
       61 gagtccatat ctgttgagtg cagaaagtcc catgtaaaat ccgtttatct tgccgcattt
      121 ttcaatgtat ttatacttga ttattttatt agggttcaaa acacttgcac caaatggcgt
      181 ttgcgatgga atatagtaaa ttcctctttc atatctgaca atctttttcg atttgcataa
      241 tttagaaagc tctttatcta tccagggtct tgaataacct ccatacttta tatcacttac
      301 agaaatcggc tcatttgttc caaacgtatc aagtaaatat tcgtacaaca tactgacacc
      361 tccttgcatt ttcattatat catattcaac catataagtc aaatgaaata taatcacttg
      421 tcagatttca ccacagcgaa ttcgtcaaaa taatcacaaa aaaagaaaag tctgccccct
      481 acaggcagac ctctccttct atccaaaagc ctcgcaaatc ctcagtgatt ctgaataatc
      541 tcgttgaaat agccgtcgat cacgctgtcc gccttgttgc gttcgctgtc gaaggtttcc
      601 tggtacacgt tcttatacac atccggtgta ctccagccgc ccatttccat ggcatatttc
      661 tctggaatgt tcagcattgc cgtggtgctg gcaaagatat gccgcagatc atgaaaacgg
      721 atctcttcca gaccgtgtgc ctccagcaat ttcttgaacc gcttgcggat ggtgtttggt
      781 gtgaacggaa caatatgatc tgttcccgac tgatggggca cttgctgtat cagtgcataa
      841 atgtatgccg gcagattgac atagcgcata ctcttgacgg ttttgcagtg gtcgatctcc
      901 gcccagccgc tgtccacctt gacgatctcc cgacgcacat agagccgcat ctgcaccggg
      961 tcaatgtcgc agaactgcag accagccact tctccaatgc gtagtgacag ccacatggca
     1021 agcaataccg gcagttcgct gcttgtcccc ttgacgatgg catagatcac atctgcggac
     1081 gggagtcttc gttcccgcac ctcctttttc ggcaggcgaa tgctgcccat tttgatggta
     1141 atatcattgg cattcagcac cgcacgcagg aagccgactg cgttcttgat ggacttatag
     1201 ccgagacgct gagcgtccac attgatggca atttgaatgt cacgggcagt cagatcttcg
     1261 attttgatgt ccatgatgta ctgcagcttg ttgcggagca tttcccgata acagcacagg
     1321 gtggtgggtt ccagcactct gcttctgctg tcaataaact gcagcatggc ttctctcaca
     1381 gtgtaaggat ttcgttcttc ttcctcaatg cccttgagcc actttgtcgc cttgaaatac
     1441 acttcgtcct ctgtgctgcc ggtaaacatt ctgcggtttc gcttgccgtc cggacgaaca
     1501 cccttggaca cctgggtgaa atatgtgccg ttttctgcct gctgaatgac cagttttgtc
     1561 tttttcattt tcataagctc cttttcaaaa tgtgcaggct ggttgaaacg tagcctgctc
     1621 tgtgtgttat gccgccaata gatcgtccag tgtcagatcg ttgatgcaga ggaacgtgtc
     1681 caccattttc agcgtaagcc ctctcgcatc atgatcgtag ttctggagcg ttcttgtgga
     1741 acagttcaga tacatcgcca actcatcatc tgtccagtcg tggagcagct gccagtagcg
     1801 aatcttgcac cagatggttt tctggagcgg aatgtgtttt tgcttgtttt tcatagccat
     1861 tgtgatttcc tcctcgttgg aatttgggat attgctttac gtctgcaaat tcagaatatt
     1921 ttatccaact gtttgggcgg ttgtattttt caaattttgt ttccgaaaaa agaaaccatt
     1981 ttccttgtgg gaaagtggtt tctttttgtc agcagtgtgt gatttgtcct attcgcagca
     2041 aatctcctct tcacaccgaa ccgcacaaag cgtttccaaa agcaagttgc tgtcgcacag
     2101 catttcctcg atctcatcag cagaataacc atagtccaac agcaaaagaa catcctcctc
     2161 tgtcacgccg tagcaaccgc acatctccaa cagcaattct tcctgttgcg tgtaatactc
     2221 ctcctccaac tcgtcatacc agttatacca cttgccaaac cggaaatttt cctgcacctc
     2281 aaaggtggaa caggtcagaa aaccatagcg atcaatcttc aagatcgtgc cttcttccac
     2341 atgcaacact tcaaacggat acttgtggaa accagacttc tgcaatgccc ttttcagaat
     2401 gctttcggtc gaagcgtaaa cgtaaagccc cagttgtgca aagtggagca ggcacatggg
     2461 attgctgcct ttgacgatgt agagcgtgtt gttctcgtcc agagcggtaa agcagaaact
     2521 gccctgaact aactctgcca tgcttttgag tgcatcgaag tccaacttgt cctgctgttc
     2581 caacagctgt actgcaatat agctgtctgt ttcaatttgc gtattcggca agtgcttttc
     2641 cacacgcagt tccttatcgt tatacagtac gccattatgg gcaaaggcaa aattgacatc
     2701 cgcatgaccg taaaaggggt gattgttgta gttgaacttc tcgttgccct gtgtggtcat
     2761 tcttgtgtgt cccatgactg cccttgtacc gttgggtgcg ttaaaatgaa tcttgtgagc
     2821 tggcttgggt cttttataga tggtgacctt gccgcttttc acataggcaa taccagacgc
     2881 atctgttcct cgttcttctg ccgcatttgc cagagcctgt gtcagttttt tcaacaacct
     2941 gtccgaaaca atgcctttgt aatccagcca accaaataat gcacacatct tacatctcct
     3001 cctctgcaca aacttcttca tttacgtaca actgacgctc tttcagatac tgaatcaatt
     3061 ctggctctgt gatgtctgcc acaaagtccg accatgacaa ctttgcaatg ctgtcatcgg
     3121 tgttgtacat tgcaacgtca cagatgcggt tgaccagctg aatggtggca atgaacgtgt
     3181 nnnnnnnnnn gtctgaactc cacggtatga taattgcaga ggttcacggc agcataccga
     3241 ccgttgccgc ccttttttgc cttgtccatg attgcttttg gggtatgctc gtaaccatag
     3301 cgagccgccc aacggttcat ggtgtattcg gaacggcggg aaaactttaa gagttcattc
     3361 caatgatgct ccacaaaata caggattctg gagatcacct cgtcctgttc ttcctgattg
     3421 tcggaaaaag cattgcggtt cacatggaga tgcaagccgc aagtgcttgt ctgatgcgag
     3481 cggtagcctt gacgaacggc atggtgcata atatcctccc acgggaattc gttaatgtga
     3541 tagtccatgg tgcatgggtg ggagaccagt tccataccat catctaacga gccatctgtc
     3601 ttgatgtaga gcaggtctgc atgggcgttg gcaatgtcca gcagctcttc cgcatagtca
     3661 tcatctctgc cagctccgtc aatttccagt tcgatgccga aatagcggtt gccttcgccg
     3721 tagaaaatgg gattcggctt gtagccgtat tcgtggatca gattgctttc ttcacgttca
     3781 tcctcatagc agtcacggca gtatgtctcg ccgtccagat agtaggcatc gtcctcatgc
     3841 aaaagagcgt cacagcaact gcatctggtg tagctgtgat ggtagcaatg gctgcacagc
     3901 gtaatatcat tatcaccata aacatcctcg tcccagattc gctcgccaca gcattcacaa
     3961 gtagtagtgt gattctccag gcagtcggaa caaatgatct gcccgttcca ctcggtgtag
     4021 tcatcgtcat caatgacaca gccacagcac tcacaataca aggtttcttc ctgttcgttc
     4081 tgttcttttt ttacttcttc gctcatttga aattcctccg taaaatgaaa atagccctac
     4141 cgaatggaat cggtagggct ttgtgtttgg gtttagtatt cttctgctaa gagcatggtc
     4201 gagtggtcga tgtcgtcaat gacatagacc ttttgattga ctgggtttgt aaggttaatc
     4261 atatagtctt gactgtactc cggctgttcg gaaaagtggt ggatgtgctg ttttccgtca
     4321 atttctttca gtgcaaggac ttgaaagtaa tctcgttctt ctggcagatt atcgatgcag
     4381 ttccacagga atagctgtaa ctccatcggt atttctgcct gcacacctct ggtcaaaaat
     4441 cgcttgttct ggaacatttg tatacctcct ttcgctggtt tgttatgtcc ctatgttccg
     4501 cccgaaggcg gcttttctat gcggtcgaca tgatcgtatc atcagtacct ggcacagatt
     4561 cacgataatg atatataatt gtatccgttg actttttcag tggattacag tgattttgca
     4621 atgtttcagt ttacacactg tggtatgttc agggtaaaaa aaatcccctg tacgctgttg
     4681 tacagggtgt cgcagagggt atgagggctg acacgaggag tggaagggca aaggcaattg
     4741 cagcgttcct ttgcccctct gctgaatccg agccctcact taatagaagt ctcctctctc
     4801 agattcagct tgtttcttct ttttcataca tcgtcttttc aggtagcgat cattcggttt
     4861 cggtggtgtg ttgatgcaaa aatatgtggt atgtttgtgt gtgaaataaa accgatcgtg
     4921 tggcggattt tccttgaaat atccgcaaaa accatggaca gcatacccta tagaaattat
     4981 gtatactgct attatccata tgtttgcttg aaagttgaaa atcatccggc actgctgacc
     5041 atttcttcca tggtgttttt gtctccccgg tcgacaaaca ccacagaaga acaaccagac
     5101 gggaaaaatg cacaactccc ttatttatat aagggcagtg tacaaaaatc ccgttctttc
     5161 tggcgttttg gtctgtgcgt gacggcgcat agcgacgacg gcacgcatgg acggaatttt
     5221 cagcaccgcc ggttttcaaa ataaaacgaa cagaactatc tggtaaaaga taatgcacga
     5281 agggagaaga taacgtgaaa tacgtcattg cactcaaaca ttttcaaacc gatccccacg
     5341 gagaccatat gctgaacctc gcctatcact acgggatctt ataccaggaa caacacggcg
     5401 atatgctgtt ctttttccgg gaggagcttg ccgacaaaag aaccgcacgg ttttttggcg
     5461 acctgctctg tgcgattttt caaacgcagt atgcagaccg aacctatgcc tatcccaccc
     5521 aatttgagcc acgctttcag atcagcattt acgatgtcct gttcgccttt ttgagctatg
     5581 taagaggctt gaccgatgcc aagcactata aggaagtgct gcgaagagaa tttgcaaaag
     5641 aaaagacaga ggtcgaagat acgctcccca tcctctacct gctgaaagac aacacctatt
     5701 ccgtcacgcc tctggatgcc tgcacctatg gatacgagac aaaaatggtc atggcgaaat
     5761 ggaagctcca cggcaagacc caagccagac agggtgtccg aaacaagcag cgaagagcag
     5821 tctaccgcaa tttcagctgg gaaaatctct atgaccgctg tccgctctac tgggatttca
     5881 ccgaaggcag aatcgaaaac acccaggaca tctattttct ggcaagaggc atgtgcggtg
     5941 cagaaaaggg aaagcaaaag tttctggaga tcatgcattc ggaaaagaat gccgaacagc
     6001 actaccagaa tatcaactgg aaggaaattc tgacagcaat catcaaggac aatctgcctg
     6061 tgccgccctg tgagaactgc gattattgtg accggtgcag ccattccgaa aatatgcttt
     6121 ccacggcgaa accgacccgg cgagaagtgc gcattttgaa aaaggagtgc tatgtggatt
     6181 tggaaagggc tcaccaggac ctgcaaaacg cctttcaaac ggcaatggca tctgcggaaa
     6241 acaagcttta cctcatcaag gggcagaccg ctttgggcaa gaccagcacc tatctcaact
     6301 acatgaaaga cagcgtgcgt cctgttgtga tcgctgtgcc gactcacgaa ctgaaacggc
     6361 agatattcta tgatgcgaac ctgcatggaa tcgaagccat ctgtgccacg ccggatattg
     6421 ccacatacag catttcagag gacgtgaccg aggagatgca ggatttttac gacatcggag
     6481 caggtgctta tgctctgcgg tttctggcgg aaacgctaca gcatatggga aaagataacc
     6541 cggattatgc gaaaatctcc cggtttttga aggactgcaa aagcactgcc agatttcagg
     6601 ggcatatcat caccacccat gccaagctgc tgcatttgcc gaaggaagtg ttccagaccc
     6661 atgatgtgat cttggatgag gacattttcc ggacgatttt ccgcacggag tctgtttcga
     6721 tgcataccct aaaaaagatg actggcagtc ggtatcttcc ggactcggta aaaagccgcc
     6781 tgaacggaat ctgcctgaaa cggggttatc atcagatgga cgagtttgtg gtggagctgg
     6841 aggaaaaaca gctgcggaag atacgccatt ttggggtgaa tctatacggt ctgctgcgtg
     6901 caaagtacat ccatgcggac agagaacggg tgacctttct gatcgaagaa tcgctgccgg
     6961 actgcaaact ggtgatgctt tcggcaacgg tttgccggga actgtatcaa aaggtttacc
     7021 ccaatcgggc aattgacttt cacgaatgcc caaaggcgaa atacaagggg cagattttcc
     7081 agtacaccga ttccagctac agccgatatg cttttcagaa tgattatgac aagattcgcc
     7141 tgctcaaaga gttgtgcaga gataccacag ttattacttt taaggacatt gaaaaagagt
     7201 tcatcacaca ctatcacttc ggcaatgtgg agggcatcaa tgcactgaaa ggaaaagatc
     7261 tgtctgttgt gggactgccc aatctggacg aggtagtgta tggcttgtat gcgatgcgtg
     7321 caggggcaag tctggcaaaa gtgcatatgt acccgcagag aatcacctat cagaacaagt
     7381 cgtttttcct caacacctac aaggacgaaa cgctccggat ggtgcagacc tggctgctgt
     7441 cctcccagct ggaacaggcg gtgggacgtg cgagactgct gcgggagaac tgccgggtat
     7501 ttgtctatgc cggattcccg gtggaacagg caaaatatat agaccgctta tgtgtccaaa
     7561 aaacggaata aaccggaaaa atccaatatg gagctgtgca ggcaaaaata acggagctat
     7621 ggcttgaaat cttttacaaa cggcctaaca tagagataaa aaaagcagga aaatacgaga
     7681 aaatgcggaa aggagtcctg taacatggaa aatgagatca tgaagtttga ggaggtcatg
     7741 gagtttctca gcgttggaaa aaatacattg tacgcactcc tgaacagcgg tgagattcag
     7801 gcgtttcgga tcgggaaggt ctggaaaata ccgagaaagt cagtgacggc gtatgtggag
     7861 gggaagcttg cggggaagga ctgaaaaagg cagagcatgc ttgtaaaaaa atacaagcat
     7921 acaaacatta tagtaatggt tgtaagaatg ctctgcagga aacaatatgc aaagcgactg
     7981 tgatgatttc atataacagg agcaggcaaa gtcctgctct tcctcctttt ttgtaaaaaa
     8041 cagagcacga attttgacat atgaagaccg ttgagatgga agaacaaaag caccaaagaa
     8101 aacaaccccg cacaaaaccc aaaaaacaag ttttgtacgg ggtcatttta tatggtattg
     8161 cggaatcctt ttccgataat ttcgctgcta ttggtaatca tcataaacgc ctggggctgt
     8221 acttttttaa tatagtttcg cagctgcaca gcctggcttc gtttcatgac ggtcagtaca
     8281 atatattcct cctgtccgct gtatgttccc tccgcggtaa acagcgtcgc acttcgcccc
     8341 agttctgtgt ggataaagtc acagatcggc gtggggtctt tgcagacaat ggtgaagtat
     8401 ttgcacaggt tgatattttc aatggcggta tcaatgacaa tggttttcgt aaacaagccg
     8461 cacagggaaa acaaaccggt ggtaatatcg aacacaaagc aggctgaaac ggcaatcagc
     8521 agatccacca ccagcaaagc ggtcccgata ttcatagagg tatacttttt caggatcatg
     8581 gcgacaatgt ccgtgccgcc gctggaggca cccatgttaa acatgattgc ggagctgatg
     8641 cccggaatgg caatggcaaa aatcagttcc agaaccggct ggtcagtcag cggtccggac
     8701 atgggaaaca gcttttctgc cgcagacagt gccacagaca tcagaacgct gatatacacc
     8761 gtctttacgc caaagccttt ccccagaaac agaaatccca gcaccagcag tgccatattg
     8821 atgataaaat tgtacgttcc cgcagacagc ggtgaaacag cactcagcac cactgccaaa
     8881 ccggtaacac cgccaaagga aaagttgttt gggaatttga aaagataggt acctgcggac
     8941 atcaccagtg ttgccagcgt cagaatggca tattccttgc acaccgatgc cgccgttctc
     9001 tcaaatcgcc tagttttttt ctgcatttgc atcgcctcct tgtatgagaa atccgtatca
     9061 atgactaaat tgcggatgtt tacagataaa ttgttggaat atagccgcag tccaatgtat
     9121 aattttatta taatctatta caccgcagct gtcaatacca attgattcaa attttctccc
     9181 cggcaaaaac tcacagatac tgccgcagtt cctgctcgta tggttcttca aagatgcagt
     9241 cgtcaatggt gacggttttg ttgatatggt ttactgtgaa acgaagtttt gcatgaactg
     9301 tcatataaga aaacagcctt gcccaaatac gcttttcata acttacaact ggatcaatct
     9361 gcgatgattc cacaacactc caaccaagct ccgtagcgac aaactcaagt gcctgaaacg
     9421 gtcgagaacc gccttgattt atactttgtg ctgttaaaat gctcaacaat tttcttttca
     9481 cagctatatt gcggaataaa acccaataat tctgttgcat aatcagctta accgggtatt
     9541 tgtttatccc gggatattcg ttttgcatga tccgcatacc aaatgacctg gttttcgtcc
     9601 aaaacacaat attgaaacag ctattataca tccacaagaa gctatcatga aatgaatcca
     9661 tcagaaaaac aaactgcatt gtgtagttcg cttttctgat atcgtccagc gtctgaagct
     9721 tacaggaaaa agtttcctgc atcaccagaa gcattttctt tttgatggca tttttgcagt
     9781 ctgtgatctc tggcgaactg tgcatttgcg caatacagtc ccaggtaaac tgatgcagga
     9841 tcgcagcgga atgcatttct tttctgtctt ccctttcctt tcgcctggaa tggggatcgt
     9901 cagacaatga ttttacattt ctgtccgcat tatagctctt gaagatcgat gacatagttt
     9961 ttagaacctt ttttccctcc cagttgtagg agatcagttt ttgctcaaac tgcaaaccat
    10021 cataaacgat cttgaataat aaacaccatt gggtaaccgc tcttttcaca tcatgagaag
    10081 atgtaatcgg ctcttctatg tttttcatgt actttatgat cttggaaaat tcaccacggc
    10141 tctgaaagaa ttcctttccc ggaatctcag gagcgtactt tgaagtaatg ccccgccaca
    10201 agcacagttt gtcttcgtcg ctgccaaaac gcaaaagctc tgtaaactcc tcaaaggaga
    10261 tctctttttt atagagttcg ttctttccaa actccttcag aactcttata tccatcacac
    10321 ggtcagccaa cactttttcc tttgtatagt caaatgtgcc gaggagcata tcgttgatct
    10381 ccgctctttt ttgcgcagtg accnnnnnnn nnngaaacgt tctttcaaat tggagtatgt
    10441 ttgcggctct tgtgtttctg ctgtatccaa atttactcct tttccattct ttggtgtaga
    10501 aagaatgcga ctcggatcga atgcaatctt ttcaacagca tctctttgct ttttcatctc
    10561 tgagagttta tcctcaaaaa cactgcctgt acaaatacct aaacagaaaa acgcattgat
    10621 atgaaaccaa ccgctgtata gcgcatctac cgtcctctca atttcatctg catttttcac
    10681 agacatttgg acatttgtgt atactatgtc aaacgcatcc ataatggcat tgttgtaatc
    10741 accgcattta tcgtacatat acatcccttt cgggtttgcg ataatatcca gaaccttgca
    10801 tcggctcaaa aactcccgtt ccggcagttc ggcatttttc gctgaatatc ggtatacgcc
    10861 aatcttttct gcaacgtaga tctgttccag cagccagagc ttgtccgacc tttcctccgg
    10921 actgtctgtt tctttgagtt ccagatccag gttgatctta ctttttatct ttgggatcaa
    10981 gccgccggct ttgttctttt cgacatagta aaaatgccgc ttgatctgtt ctatggtgct
    11041 tggactttcc tctgcctcgc catattggtc agcgtgttcc tgccggtaca tatcataaga
    11101 atcccatatg tctgctatgg tattataaag tccttccatc gatgccatac ggatgcaacc
    11161 ccttttgttt tacaagatat ctatatcata ccacatatcc cagggaaaga aaagaggatt
    11221 tgaaaaaaac tacaaaatca attttcttcc ttatagcctc ccctgaaagg aggaggtggg
    11281 cggcaaagcc gctcggaggg gttttccttt gcctggcagc actataaatc tcttttccaa
    11341 aattttttta cctggcacac ggcacagccc caattccctc acccgcataa tttttacccg
    11401 gcacacggca caagagcgtc ctccgaaaaa aatccggagg atttttgttc ccctttttcc
    11461 aaaaacgcat cggagaagtt tgattttcgc ccaaacagga acttttccgg cggcaatttc
    11521 agaaaagggg tacaaaaaca aacggaattt gatttttttc tgtgccatgt gccaggtgtt
    11581 tttttgtccg ttttatattt tccgcccctc cccccagagc gcctgcaaaa aaataagagc
    11641 gttttgtctg catgaaattt tccgatgtgc tataatgctt tttggaaata cagaaccgcc
    11701 tgtgccgtgt gccaggtaaa atgtatttca ataaagtttt taggaggtcc cctatatgaa
    11761 cggaagtcca aatatgcaaa taactccgga tgtcaatcag gcgcttgcaa acgcaaacat
    11821 ccctctctca cctgcattaa cgcaatattt tcagcaaaac gtcacgccat atctggatca
    11881 gctacagtat caaatgtatc agacacaagt cagccttcag gatacgctcc aatttttcgt
    11941 gaatcaaaat cgatgtcagc ttgcagaaat tgatcgactc aaacaagttc gacaaacaat
    12001 gccccggatg ttttgcgaac gtcaaataga tggttatgga gtttgcatag atcatggaaa
    12061 ccaaacccta gtcggcaagt taagaattcg cagtgttcac cattgctata tcgacaaaga
    12121 cggataccga aaagaacttt tgtatgtctt atattccagt atggacgaca acatccattc
    12181 tgcggtcgtt cctcgtgaca aattggcaag caagaatctg ttgcctctgt ttgaatcttt
    12241 tcaatgggtg tgcaaaagca aagctatggc aaacgattat gttgcgcact gcatcaacaa
    12301 ttttgctcag aaacgtattc tgtacatttc agaatatcct ggcttttctt ttgtgaaaag
    12361 caaagaagga agtacgcttg ttcaattttg ctgcaacaga aatcagtttg ggatgaatct
    12421 gctgaaacac tgctccccct atttcaccaa aaaagttctt cccagtacaa aagaccgccc
    12481 tctcggtata aagggaatct gcgaaaagta ccttgacacg gacaaaaaga tgatgctctt
    12541 cgttttttcc ctctgcggtc tgctgagttc ttttttccgg gaagagaaat acagcacgga
    12601 acgtattctg accatatctg cacctgatgc cgcatcggaa cggtttgcga cgctcctcat
    12661 gcagattttc aacagagaca caaagccgct gaccctctgt tccaacaaga gtgctgtgaa
    12721 aacggaactg ctccacgcaa aggatgaaac cgttatcctg agcgaccgca gcattatcga
    12781 cgatgacaaa aaaagaacag aaatgttgca gcacatccta acggaaaatg acagcatgga
    12841 ttgtcagccc cataatcttg cgattttctc cacccatgca caatacctgc tccctccgga
    12901 gaaaaagatc tgtctgacgc tgccggaaaa ttttgcccct gctatgacaa aggaagaaga
    12961 ggcggagatg tgtgatgatt tgaattatct cataaacacc atagcatctt acatctgtaa
    13021 aaactacgaa ctgtttcgag ataggttcaa acaaaacttt atcgatatct tcaactctga
    13081 attcgagcaa tacagtccct atttccaagg caaaagcaaa gaaatcatta cggctggtgc
    13141 attactggat accgtttact tttgcgtgat agnnnnnnnn nnaaaaaccg ttatccgctg
    13201 gtggtgtatt cccaccacag aagcctgcgg cctgctctga tcgccctgaa aagcgaccgg
    13261 attctggatt ccgacgtgga actcatgatc gaggaaagcc gctatgcaga atggtacagc
    13321 acagcacctg caccggagca ttttattccc ctcacggaaa accgcatcgg cggtgtttcc
    13381 taccaggtct ttgacgaaaa acgcaaggat aatatgcact tctttgcact gggaaaaagc
    13441 ggttccggca aaacacactg tctcaccgaa cgaatggtga gcctgcaaaa gctgcaccgt
    13501 cccgttattg tctttgatac cagtgaatcc tttacagaag aggagatact ggaaaagctg
    13561 tctgtaggct gcggtgaaac ggcagagaag aaggttcagg cgtatatcgc cgagcatatc
    13621 accttccacc gcatcgaaga ggacggaatc cccgttgacc tgctcaaact gggcgactcc
    13681 ggcaatgggg aagataccat ccgaaaaatc gagtccattg tggaatctca caaccccaat
    13741 atgggcagca agcagcaggc tgctgtgcat gctgccatca gcgatatgat ccagaaaggc
    13801 aatgtggata tgacatctct gtatgaggtg ctgacaagtg aacagatccc gtatgatctg
    13861 gcagatcagc tggcggatac gctctccttt tttgtgaact tcaaggcaaa cgacagagac
    13921 tggggcgagc tgatcgaaga gagcaaggat attattatca tttccaccaa ggcggtacgc
    13981 tctaatggcg gttctggtct gatcgatatg ctgctgatgt ctctgtatta ctatcagcag
    14041 gcacacggcg agaagcagct cagcgtattc atcgacgaga tcagaacgca aaacctcagc
    14101 accaaaggcc ccatcgccaa gattctgacg gagagccgca agaaccgcat gagtctgaat
    14161 tttgccaccc agtttctgcc caagtccgcc caggtccggg agatcatgga caatgcagcg
    14221 atccatgcct ttttcccgct ggacgacaga accgccgcct ctgcggcaaa gctgctcaat
    14281 atcgatcccc aggcgctgac cctgctggaa acgggcgaat gctatatcaa gggaacgttc
    14341 tacaaccaga acagacagaa agcgatcccc ggcattctcc acggcacaac ataccgcaat
    14401 ttcaagaaag ccaaaccgaa attgcacaaa cgtcccttta ttgatgtgcc ttgctttgac
    14461 gaaaaacgct gcaattttcc ccctgcaaaa aattaagagc gttttgtaag acggcattgc
    14521 aaaagtgtgc tataataccg attgtacaga ggcgacggca gcatatcgct tgccgcttct
    14581 gaaaaaatga aaggatctga tcagtatgaa cgaaacagtc aacacccaca agaagatgga
    14641 agaactggac agaatgagca ttccctatga ggcgatccca ccttccgaat ggaagcgcaa
    14701 cgcctacgac agcaacaaca gcgtcagcat agatacatta tgtatttttt acaaatgtgc
    14761 aagagatttg gaaaagataa tagaggaaca ggagggtgac gaaaaggagg tcgaagaata
    14821 tatcatcaat ctcctctcat tgatactgac acaaaagctc catctcatcg tctaccatga
    14881 cagatggctc tatttcttca acggaaaata ctttgaaccg tgtccgtcta aacaagaact
    14941 gcagtgtatt ctccgtgaac tgtgtgaggc agctgcttac ctttggaaac gtttttccgt
    15001 acacgacaag accgtaaagc gattgctgag ccatgtgcag caaaacgctg tcaaagtgga
    15061 ttatccgccg gatatcagca agtatgttgt ctttgaaaac ggcgtcctga atctggacac
    15121 cttagagctg gaagacttta caccgcagaa attctttacc agcatgatcc atgcggactg
    15181 gcttgcggaa aacaaagcct gcccggaata tgaaaaggtc atcaacacct acaccaatca
    15241 cgaaatgccg ctgaacaagn nnnnnnnnna atcaacgaag tcctgggcgt ttgcctgacc
    15301 aacgatatgg tcaagaagct gttctgcttt ttgggtgtga ccaactccgg caagaccctt
    15361 ctgctgagct ttctgatgcg tctgttcaac aactcagcaa ccgttaccat gcagccaaac
    15421 gacctgggtc gccagtttgc gcccagcatg gtctatggaa aatccctctg ctgctgcatg
    15481 gacatggact ccgcaccgct gaataccaag gcgactgcct ttctcaaggg catctccggc
    15541 gctgatatgc tttcagcgga attcaagggc gaaaacggca gtgtcccgtt ccaaagccgt
    15601 gcacacatca tcaacgcctc caactacgac attaccacga agatgccgga tgcggcattt
    15661 gatatgcgta agcttgtgat cccgttcaag tacagactga ccgacaatgc agagcccttt
    15721 gaggtactga tggacaagct ggagcgtgag aaggcagcca ttgtgagaaa gctgattctg
    15781 gcgtacaagg cgctcaagga caaccactac gaattcagcg actccgaaga aggcgaaagc
    15841 tatgacagct acgtaccgcc cacggcagtc agccgcagca ccagcaagtc tctggacttc
    15901 ttctttacgg cttgttatgt acgcaccgag aacgacgatg actacatttt caccgaagat
    15961 atgtacgccg attacaagga atatgccctg gatgagagct atgcgtactg cttcccgaac
    16021 tacgatgcct ttgcaaaggc tgtcagagcg gagctgcaac tgaagtccgg ccgcaagcgt
    16081 aaaagcagcg atgccaaccc caagcgctgc taccttggca tcaagcgaaa agcactggtg
    16141 acagaagagc aggcggaaga ctgagtttcc gctccgaaag cacaaatcac ccgaatttaa
    16201 aatgattttt tacccgatgg aaggtgatcc atcgggcttt tttgatggtg cagaaaatgc
    16261 tgctttttcg ttccccagcg tgaattcctg cgaaattggc agatgttccg gcgggaaatt
    16321 gacaatatga acagaaaaaa ttaaggaaac gctgaacaaa agcacagttg aaaaagcaaa
    16381 tcgataatta cgacta
//
LOCUS       DAWENO010000033        15956 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1526_length_15956_cov_10.701088, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000033 DAWENO010000000
VERSION     DAWENO010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15956
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_15956_cov_10.701088"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    620..629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3728 a         4503 c         4122 g         3593 t
ORIGIN      
        1 gagaccggcg ctgccagctc gatgtgaccc atacgctcac ggcgtacctt tgcacgggtg
       61 acttctacgc cgcagcggtc gcagatctta cccttaaagc gaatcttctt aaacttaccg
      121 cagtggcact cccagtcctt tgtcggtccg aaaatgcgct cacagtacag accgtcacgc
      181 tccggctttt gggtacgata gttgatggtc tccgggcgct ctactgcacc ataggaccac
      241 tcccggatgc gatccggcga agcaagacaa atcttaatag attcaaaagt gttaaattcc
      301 aaaccgttac ctcctaatat caacgaggaa acaggagaag cgaacgcagt ttcgtccgct
      361 gcctgtctgt tggtgtatct ctatagaaat ccgatctttt tccgggcgtt tccacaccca
      421 tgaaaagcag atgattacat cagatcgtcg tcatccaggt catcatccag accgaaatca
      481 tcgccgagga gatcgtcgtc atcgtcctca tccatagcgc tggtctgact gccgcccagg
      541 ctgctgccca ggaggtcatc gtcgtccgga ttgccttcct taaaggagag acctgcgtct
      601 tccatatcct tatccacgcn nnnnnnnnnt atccacgccc tcatacagca tggtatcctc
      661 tgcatcgtct gcaaagctta ccggctgcgg aatgattgcc tcgtcctctt cgaagttgga
      721 cttgaggtcg atctcttcct ggttgctgtc cagcgccttg acatccagac ccagggactg
      781 catttccttg atgagtacct tgaaggattc cggcagaccc ggcttcggca cattctgacc
      841 cttgacaatt gcctcatagg tgttgacacg acctacggta tcgtcagact ttacagtcag
      901 gatctcctgc agggtgtatg ctgcgccgta tgcctccagc gcccatactt ccatctcacc
      961 gaaacgctga ccaccgaact gcgccttacc gcccagcggc tgctgggtta ccagtgcata
     1021 cggaccaacg gaacgtgcgt ggatcttatc gtctaccagg tggtggagtt tcagatagta
     1081 catatatccg acagtaacac ggttgtcgaa cttttcaccg gttctgccgt catacagcgt
     1141 gaacttgccg tcctcgttca tttccagtgc cttcgggttg atgaccttct gcatgggttt
     1201 cagatcgaac ggatcgtcac ggtgcggtgc attcttttcg tttgcctcac ggaagcaagc
     1261 acggatatca tcctcgtgtg cgccgtcgaa taccggcgtc atgatgtgcc agcccagtgc
     1321 cttggcagcc atacccagat gcacttccag aacctgtccg atgttcatac gggaaggtac
     1381 gcccagcggg ttcagcacga tatccagcgg tgtgccatcc ggcaggaacg gcatatcttc
     1441 ttccggcaga atacgggaaa cgacaccctt gttgccgtga cgtcccgcca tcttatcgcc
     1501 cacagagatc ttacgcttct gtgcgatata gcagcgtacc agctggttta cgcccggaga
     1561 cagctcatcg cagttctcac gggtgaagat cttgacatcc acgatgatac cagcctcgcc
     1621 gtgcggcacc ttcagggagt tgtcacgaac ttctcttgcc ttttcaccga agatcgcacg
     1681 gagcagacgc tcttctgcgg tcagctcggt ctcgcccttc ggggtgacct ttccgaccag
     1741 gatatcgccg gcgtgtactt ccgcaccgat gcggatgata ccgttttcgt ccagatcctt
     1801 cagcgcatcg tcgcctacgt tcgggatatc acgggtgatc tcttccggtc ccagcttggt
     1861 gtcacggcac tcgatctcca gttcctcgat gtgaacagag gtaaagacat cttctcttac
     1921 cagacgctca ttcagcagaa cggcatcctc gtagttgtag ccttcccagg tcatgaaacc
     1981 gatgagggca ttcttaccaa gagaaatttc accgtcagca gttgccggac cgtctgccag
     2041 tacctgtccc ttgtggacac gctcgccggc atccacgata ggacgctggt tgatgcaggt
     2101 agactggttg gaacgcttat acttctggag tacatattta tgggtcttgt gttcgtcatc
     2161 caccaggacg atctcgtctg cggaaacaga gtcgatcacg ccgtcatact cggaaacaac
     2221 gcagacaccg gagtctacgg ctgccttgta ttccataccg gtgccgacaa acggacgctc
     2281 ggtcttgagc agcggcacag cctgacgctg catattggag cccatcagcg cacggtttgc
     2341 gtcgtcgttt tccaggaacg ggatcatgga ggttgcgacg gagactacca tcttcgggga
     2401 aacgtccatg aagtcaacac gctctgcctc aatttccagg atctgatccc ggaaacggcc
     2461 tgcgaccttt gcatttgcga agtgaccgtc ttctgtcagc ggttcgttcg cctgtgcgac
     2521 aatgtagtta tcttccatat ccgcagtcat gtagactact tcatctgtaa cgcagccggt
     2581 ctccttgtcc acacggcggt acggtgcctc aataaaaccg tactggttga tccgtgcgaa
     2641 agttgccaga taagagatca gaccgatgtt cggaccttcc ggcgtctcga tcgggcacat
     2701 tcttccgtag tggctgtagt gtacgtcacg tacctcgaat ccggcacggt cacgggacag
     2761 accgcccgga cccagtgcgg acagacggcg cttatgggtc agttctgcca gcggattgtt
     2821 ctggtccatg aactgagaca gcggagagga gccgaagaat tccttgattg cggaagtgac
     2881 cggacggatg ttgaccagtg cctggggtgt gatgacatcc atatcctgtg cctggatgtt
     2941 catgcgctcc cggatcacac gctccatgcg ggtgaaaccg atgcggaact ggttctggag
     3001 cagttcgcct accgaacgga tacggcggtt gcccaggtgg tcgatgtcgt cgatgttgcc
     3061 gacaccgtcg cacaggttca ggaagtagct gatggtagca tagatatcat ccaggatgat
     3121 gtgcttcggc accagttcat ctgcacgctt gcggatggcg ctgcgcagtt cgtcctcgtt
     3181 cgctgcactg tccaggatct cacggagtac ggcatagcgt accttttcgc tgatgcccag
     3241 ctcctcgcag tcaaagctga catacttatt gatatcgacc atgccgttgc cgatgacctt
     3301 gacttccttt tccacgaaac ggatctcgtt ttcgccggac tcggtcacga agtattcctt
     3361 ggtctcagcc ttgatgaatg cctccttgac gcctgcctcc tggatctgca ttgcctgatc
     3421 gaaggacagc gttacgcctg cctcaaagag gatctcgccg gttgcctcgt ctaccaccgg
     3481 acgggtcagc tcgtgacctg ccagacggct ggcgatgccc agctttttat tgtacttata
     3541 gcggccgaac cggcagaggt cataccgctt tgcatcaaag aacaggttat tcagatgtgt
     3601 cagagagctt tccaccgtag gcggctcgcc cggacgcagc tttttgtata cgtcgatcag
     3661 cgcatcttcc gtgttcttgg tggtatcctt ctggaggatg gtttgggtca gacgctcgtc
     3721 gctgccgaac atctctgcga tctcctcgtc agaaccgatg cccagggaac ggataaaggt
     3781 cgtgatcggg atcttgcggt ttttatcgat gcggacatag accacatcgt tggaatccat
     3841 ttcatattcc agccatgcgc cacggttcgg gataacggta gccttgaaca ggtccttacc
     3901 ggtccgatcc ttttccgatg cgtaataaac gcccggagaa cgcaccaact gagaaacaat
     3961 aacacgttct gcaccattga tgacaaaggt accgctttcg gtcatcagcg ggaagtcgcc
     4021 catgaagatc tcgctctcct tgacttcacc ggtctccttg ttccagagcg ttgctgtcac
     4081 acgcagaggc gctgcatagg taacgtcacg ttccttgcac tctgcaattg tatatttggg
     4141 ggtgtcatcg atccggtagt ctgtgaagtt cagtaccaga ttgccgttgt aatccgtaat
     4201 ggaagaaacg tcacgaaaga cctctttcag tcctttttcc cggaaccact tgtacgagtt
     4261 cttctgtacc tcgatcaggt tcggcatttc cagaacttcg ttgatcttac cgaagttcat
     4321 gcgaacgttt ttcccgcact gctttggcgt aacttgcacc attggcgaaa aacctcctta
     4381 actctttgtc agacaaaaat ccgcctgtca ccttttcgcc gcaccggacg aaaaaacttt
     4441 ttgctttttt gaaaaaagtg cttgacagac gagggtggaa tgtgttatac tattctgcta
     4501 agaaatacat ttacacatta cgatacattt tattattata taatattggc gtcctgttgt
     4561 caagggcgtt tttttatttt tgtaggatcg cagccgaact tcttatattt cacagctttt
     4621 ttcatcaatt tgcacaacag caaaaaacgc accctccgcc gtgtatcctg cgtccgaaaa
     4681 ttttttcaga ttccgactgg tacaccgttt acatttttcc gaaatcatgg tatactgata
     4741 agagcctgtt tcataaagcg aaacagcggc agcgctgtgt gcagcgctgc gcaaaaccca
     4801 aaagcgaggt gaaacgcttg tttacccaaa tcaaaagtct ggggctgttc gggctgaatg
     4861 cctttccggt ggatgtggaa gtcagcattg gacgtggcaa gccccagttt gaaattgtcg
     4921 ggctgccgga tgcctctgtc cgggagagcc gggagcgtat ccggtcggca cttgccagcc
     4981 tttccatcgc cctgcctgcc cgtcagatca cagtcaacct tgcgccggca gatgtgaaaa
     5041 aggtcggcac gatgcacgat ctcgccattc tgctgggcat cctggtctgc atggaacgca
     5101 ttccgccgca gccggaaaac cgctgttttc tgggtgaggt ttccctgtcg ggacacgtcc
     5161 ggagcatcag cggcgttctg cctatggcac tgctggcggc acagtccggc atccgggaac
     5221 tgttcgtccc ggaggaaaat gccgccgagg catctgcggt aggcatcccc atctacggcg
     5281 tccattgtct gagcaatctg ctcacccatc tggaggacac cgcaagaccc attctccggc
     5341 agcagccgac ctatgtgccg cagccggagg actatcagga caccctggat ttcgccgatg
     5401 tcaaaggaca gcaaaacgcc aagtatgccc tggaaatcgc cgcagccggc ggtcacaatg
     5461 cgctgatgat cggcagcccc ggcagtggca agagtatgct ggcaaagcgc atcccgtcca
     5521 ttctgccgga gatgactgcc gcagaatcca tggagaccac ccagatctat tccatcgccg
     5581 gactgctgga cagcggcaag ccgctcatga caaagcggcc gttccgtgcg ccccatcaca
     5641 cgatctccgg cgcaggactg gtgggcggcg gcacgattcc ccgaccaggt gaaatttccc
     5701 tggcgcacaa cggactgctg tttctggatg agctggcgga attcgaccac cgcaccctgg
     5761 agatcatgcg ccagccctta gaagatcgga gagtcaccat cacccgtgcc tccggcacag
     5821 tttcctatcc ctgttccttt atgctggtgg gcgccatgaa cccctgtccc tgcggctatt
     5881 acgggcatcc caagcggcaa tgcacctgca ccgaccggca ggtgcggcag tatctgaacc
     5941 ggatctccgg gccgctgctg gaccgctttg acctgcatat tgaggtggag ccggtgtcgt
     6001 ttgactccct gtccgccagg gcaaaggcag aatcctctgc cgccatccgg gaacgggtac
     6061 aggcggcaag agaatggcag aaccagcgct tttccggaac gggcattttc tgcaatgccg
     6121 ccattcctgc cggaatgctc caggacttct gtcccatgga tgacggggca gtcaccctgc
     6181 tgcgtgccgt gttcgacaag ctggggctga gcgcacgggc atatgaccgc atcctgaagg
     6241 ttgcccgaac catcgccgac ctggacggca gcgacatcat ccggaaacaa cacattgcat
     6301 ctgccgccca gttccgcagt ctcgaccgaa aatactgggg aaccggcgga taacccattc
     6361 cacgaaagga attctcatga accgagttta taaatctacc aaacgagaaa agccggtcag
     6421 ccctctggtc tcctactggc ggcggctgga caagccgctg atcctggcgg cgctgctctg
     6481 ctccctgctg agcatcatcc tgctgtactc catctggcag aaccacatca tcagctctgt
     6541 gggtgcaagc tattaccgga cacagctgct ctcctgcggt ctgggtatgg tgtgtctgct
     6601 ggtgctttct gccattgact atcacaagat cgtccggctg tggtttctct atgcgcccat
     6661 cgccctggtg ctgaccctgc tcacctttac ggcgctgggt cacaggcgtg ccggcgcaga
     6721 cgaccaggcg tggctgaacc tgggctttat ccagttccag ccatctgagg tgctgaaact
     6781 ggcgtttatt ctgacgtttt cgctccatct cgccaaggat gagggcaaca tgaacaaacc
     6841 gctgcatatg ctcctgctct gcgtccacgg tgcgatcccc accggactca tcgcactcca
     6901 gggcgactat ggtactgcca ttgtctttgc ggtgatcttc ctgggaatgc tctttgccgc
     6961 aaagatttcc tggaagtatg tgctggcatt tctgatcgcc attccggcgg cgctggtcgt
     7021 tgcatggaac ttcctgctgg gagacaccca caaaaagcgt atcctggtac tgctccaccc
     7081 cggcacagac ccggaaaata tcgagtacca gcaggaccgt ggtctggagg cgctctccaa
     7141 cggcaagatc ttcggtgtcg gtctccacgc cggcaaggaa aactatgtgt ccgtgccgga
     7201 aatacacaac gactttatgt tcgcacaggt gggacaggcg ctggggttcg tgggcgctgt
     7261 cgctgtcatt ctggtgctga cattcctgtg cctcaagatc ttgtacgacg gattccgcac
     7321 caaggacaaa atgggaatgt ttctctgcac cggcgtgttc agtatgctgt tcatccactg
     7381 cgtcatgaac atcggcatgg tgctgaaagt ggcgccggtc atcggtgttc ccctgccgtt
     7441 catcagcgcc ggcggtacgg cgactgtgag tatgtacatc gcactgggat tagtggtcag
     7501 tgcctatgga cataacgaca agaaataccg ggtgtttgac tgattcgctg gatgtgtttt
     7561 ccaattttgt tgcatggatt ccgcaaaaac gctcttgaaa atttcctttt tccggaataa
     7621 aatagattgc ggcgatgccg gacggctctg ccctaaagtt caagtttcgg aggaattttt
     7681 tatggatacc actgcaaaaa atctgcacac cctgcgtgga gaatggatgc tggcgctggc
     7741 ggtctgcatc aacagtttcg gcgttgtgct gatgctctat tccggtgccg gcatttcggc
     7801 gatctccagc gttccctttg ccttttccga ggtatttccc aagctgaccc tcggcacatg
     7861 gacgtatctg tttcaggggc tactggtgct gagtctgatg gtaatgcgca aacgatttgt
     7921 gccgccgtat ctgttcagct ttgtggtggg ctttgcattc agcgagctgc tggatgtcca
     7981 cgagctgtgg atcgggatac tgcccacggc gctgccctgg cgcatcctgt attttgccgt
     8041 cagctatgta ctgctctgca tcggcattgc gctgtccaac cggtgcggtc tgcccatcat
     8101 ccccaccgac ctgtttccaa gggaactggc agatatcacc ggcgtcagct acccgaagat
     8161 caagatcggt tttgatgtca cctgtctggc ggtcactgcc gggctgacct ttttctgtct
     8221 gggacatctg gaggggctgg gcatcggcac agtcctcgcc gcattcacca tgggaaaagg
     8281 cgtgggcatc tgcggcaatt ggatggacaa gcgctttcag ttcaccacat tcctggcgaa
     8341 acgaaaggag aacgcacaat gaaaccactg cgccttctgg tcacctatac cgcaaagccc
     8401 ggtcagcggg atgccttcct ccgggcagtg ctgcaaagcg gcgtgccgga aaccgtccgg
     8461 caggaggacg gctgtctgcg gtacgactat ttcctgtcgg cagaacgtcc ggatgagatc
     8521 ctgctgatcg aggaatgggc atcggcagaa cagcagcaga cacatctccg acagcctcat
     8581 atgcagcagc tgaaacagca gaaagaacag tacatcgcag aaacagacgt gcagacgctc
     8641 tgaatttctg gattttcaca aaatatactt gctttttttc ggaaaaagtg gcataataaa
     8701 aagaaaggat ttcgctgtgc aagcgaaagt gacacagaaa ggaaacgtaa tctatggaaa
     8761 atcaactggt tctggtactg gacttcggcg gtcagtacaa tcagcttatt gcccggcgtg
     8821 tgcgggaatg cggcgtatac tgcgaagtga tccgctatac cacaccgctg gaagagatcg
     8881 cagcacggaa accggtgggc atcatcttca ccggcggtcc gaacagcgta tatgacgaaa
     8941 aatcacccca tatcgccaag gaaatctttg aaatgggcat cccgatcctg ggcatctgct
     9001 acggctgtca gctgatggca tacaccctgg gcggcacagt caccacctgc accacttcgg
     9061 aatacggcaa gaccgagacg atctacgaca agtccagcgc tctgttcggc agcgaagaac
     9121 tgccggaaca ggcgatctcc tggatgagcc acaccgacta catctccgag gcaccggagg
     9181 gctttaccgt cacagcacat accgagaact gcccggtggc tgccatggaa aatccggaaa
     9241 agaaactgta tgccgtacag ttccacccgg aggtaaacca cacccagtat ggtctgacca
     9301 tgctggacag tttcctgaaa aaggtctgcg gctgcaccgg tgactggact atgggcaact
     9361 acgccaagac tgccattgca cagatccggg aaaaagtagg cgacggcaga gttctgctgg
     9421 cgctgtccgg cggcgtagac agctctgtct gtgctgccct gctgtcccgt gcggtcggca
     9481 aaaagctgac ctgcatcttc gtagaccacg gtttcctgcg gaagaacgag ggcgacgagg
     9541 tagaggctgc ctttgcggac aaggacatca acttcatccg tgtcaacgcc aaggctgcct
     9601 ttatggaaaa gctgaacggc gtttctgacc cggagaccaa gcgcaagacc atcggcgagg
     9661 agttcatccg tgcctttgaa cgggaatcca aaaagctggg tacggtagac ttcctggcac
     9721 agggcaccat ctatccggac gtgatcgaaa gcggttccgg cgatgcagct gtcatcaagt
     9781 cccaccacaa cgtaggcggt ctgccggact atgtggattt caaggaaatt atcgagccgc
     9841 tgcgcctgct gttcaaggac gaggtgcgtc agctgggtat tgagctggga ctgccagaaa
     9901 acctggtatg gcgtcagccg ttcccgggtc cgggtctggc gatccgcatc atcggcgaga
     9961 tcacagacga aaagctgtcc attctccagg atgccgactg gatcttccgg gaagagatcg
    10021 caaaggctgg tctggatcgg agcatccacc agtatttcgc cgtgctgacc aatatgcgtt
    10081 cggtaggcgt tatgggcgac ggcagaactt atgattatac cctggcgctg cgtggcgtca
    10141 ccaccaccga ctttatgacc gcagatttcg cacgcatccc gtatgaggtg ctggaagtga
    10201 tctcggcacg tatcgttaac gaggtaaaga atatcaaccg agttgtgtat gatattacga
    10261 caaagccgcc ggctacgatt gagtgggaat aaccctcaac acaatttcct tacaacaaca
    10321 caaagccgtc tggtctacag cgaccagacg gctttttcat atgctcacgc ttactgcatc
    10381 ttactgcagc ttatcaccgc agcctccagc gacgtgcgca ccaccagtga gatacagcgg
    10441 atgctcttct cgtcctgcgc tgcatccctg gatttcagca agtccaccag ttcccctgcc
    10501 atgccctctg tatagaagtt gcagatgatc ttcttggcat ccgggtcgga gtgtacgttt
    10561 tcctttcgct cgatctgctc cacaatggac atcacaatgc tctgaaaatc atggttcaga
    10621 aagcgtttca gctcgtccct gccgatagaa tcgtaggcgc agtttatcat gtggctgttt
    10681 tcccggatat accgaaccgc aaaccgcagt gcctcctcca gttcattcag cagatcgaac
    10741 tgctttacca catccaccgc ctcctgctcc aacgtccatt tcagcagatc gggaacgtcc
    10801 tggaagtgat aatagaatgt ctttcggttc agtccgcagt cggtgacgat atccgttacc
    10861 gtgatcttcg acagcgactt tttctccatg cagtgtttga gcgatgcgga aagcatcttt
    10921 tttgtattta atgtggttac ttcatgtttc atgaccgttt ctcctgtatt tcagcattca
    10981 gtgctgcctt tattatacat ctgtccggca aagaaatcaa ccacaaatcc cggacatttg
    11041 cccatcaatt tcaccacatt tgtccgaaat tcacccaaac gcccgatatt gaccgttgca
    11101 attttacgga caagtgggta aaatagaatt ataaaatttg aaaaggagcg atgaaaaatg
    11161 gcttacatcg aaatgataaa cagctgcaag acctatcaca tgggcggcag caccatctat
    11221 gccaaccggg acgtttcgtt ctctgtagaa aagggcgaac tcgccatcat cctgggctct
    11281 tccggcgccg gaaaatccac ggtgctgaac atcctcggcg gcatggactc caacgacagc
    11341 ggcaaagtca tcatcgacgg caaggacatc tccaaataca gccgcacaca gctgaccgca
    11401 taccgccgca ccgatgtggg atttgtgttc cagttctaca atctggtgca gaatctgacg
    11461 gcaaaggaaa acgtagagct tgcctctgaa atcgtggaac acgccctgga tccggtcacg
    11521 gttctgaaag acgtagggct tgccgaccgc atcaacaact ttccggcaca gctctccggc
    11581 ggtgagcagc agcgtgttgc cattgccaga gccattgcca aaaacccgaa gatcctgctc
    11641 tgtgacgaac ccaccggcgc actggactat aacaccggca aacaggtgct gcaaattctc
    11701 caggacatga gccggcagaa gggtgcgact gtcattatcg tcacccacaa tgcggcgatc
    11761 gcacccattg cagaccgggt catccatatg cacgatgcac gggtcagccg cattgaagaa
    11821 aatcccaacc cgaaaaaaat tgcagatatc gaatggtagg tgcatggtca tgaagaaaaa
    11881 agcgctgaac caagacattc aaaaaagctt ttccaagtcc aagggacggt ttatctccat
    11941 cgcctgcctc attgcgctgg ggtcctttgc cctggtggga ttacaggtca ccggaccgga
    12001 tatgcgaaag accaccagcg actatctcca gcagtatcac gccgcagacc tgtccgtcat
    12061 cggcagcatg ggcattgacc aggaaaacgc aaaaaccatc gacagcctgg acggaacaga
    12121 acaggtcacc tacggctatc tgaaagatgt ggtgctgaaa gatacctcgg aaagctgccg
    12181 catcttctct ctggatgagg acatctccaa gtatgaagtc atcagcggca gactccccga
    12241 aaaggcagac gaaattgcat ttgccgacgc cgacaaggac aattaccaga tcggcgacca
    12301 gatcacattc gaggaaaagg cagacacctc cgggaatacg gtactgaaaa acaagacctt
    12361 taccattacc ggatttgtcc actccacgga aattctctcc tgcgtcaacc aggggcagtc
    12421 caccgccggt tccggcgaac tgaacagcta tgcggtggtg acagaggatg ctttcgactg
    12481 cgattactac atgatcgcaa gaatcacctt ccgggacacc cagggactgg acccctattc
    12541 cgatgcgtac accgacaaga tacaggcgca caaaaaccag ctggaaaccc tgctggaaga
    12601 tgccccggca cagcggcttg atgccgttag gtcggagtat caggaaaaga ttgacgacga
    12661 cagacagcag atcgccgagg cggaaacgga actggatgac gccaagacca aactggacga
    12721 tgccaaagcg cagatccagg atgctgaaca gcagattcag gacaaccagg cggaactgga
    12781 cagcaaggtc agcgatgccc agcagcaaat tgacgagaac cagaaaaagc tggaggactc
    12841 ggaaaagcag ctggcagacg gcaaaacaca gcttgccagc gcaaagacac agctggatgc
    12901 ggcacaggcg aagatcacag cgtccgaaaa tcagatcgct gccggaaaac aggaactttc
    12961 cgcaaaacag gcggaataca acacccaaaa ggctgccttc acagaaaaac agacagaata
    13021 tcagcagaac gccgacgcac tctccgctgc acagacagaa ctggacagca accgctctca
    13081 gctgaccggg gcaaagtccc agtatgaaag cggcattgcc gaactgaaag ccggcatcgc
    13141 ccagttacag caggcggtgc agaatccgca gctttccgca gaagaaaagg cacagtatac
    13201 cgcacagctg gacgatgcgg aaagtcagct gaatgatgcc gccgcagcgc tgacaacggc
    13261 aaaaaaccag ctgtcccagg catcggcaca ggtcaacacc gccaaatccc agcttgccgc
    13321 caagcagagc gaatacaaca ccaaagcggc agaactgaaa agcggcgaac agcgggtgga
    13381 tcgcggcagg acggcgctgg cgcagggaca gcagggcgtg gaggcccagc gttctgacgg
    13441 cgaggcaaag ctcctggatg ccagagaaac gctggcggaa aagaaacagg aataccggga
    13501 aaagctggca gaatacaacg acaaaaaggc aatgccgaca gcgagatcgc agacaacagc
    13561 gcagaactgg acgatgcgca ggcggatgtg gataagcttt cgctccccac gttctcggtc
    13621 tacacccggc gtgaagttcc cggcgcagag ggctaccgca cctacagcag cgtctccgac
    13681 attgtggatg cactggcaga cgttttcccc atcttcatgt atttcgtggc ggcacttgtg
    13741 accctcacca ccatgacacg ttttgtcgac gaagaacgca tcaactccgg cacgctgaaa
    13801 gccctgggct atgaagagcg tgacatcatc aaaaagttct ccctctacgg tctgttttcg
    13861 ggactgacag gtgcggcgac aggcattgca gccggtctgt atctgctccc cagaatcgcc
    13921 aacaacgcct atgctcacgg gttcaccgtg ccgaagatcg aaacaccctt ccatctgaaa
    13981 tgggcggtta tcgccgtgat ccttgccatg ctgagtaccg tgcttcctgc ggtgctggtg
    14041 gcaaagaaag agttacagga aaagccgtct gctctgctgc agccgaaacc gccggcaaac
    14101 ggctccaaga ttctcctgga acgcatcaca cccatctgga accgcatgag catttctgat
    14161 cccgtttgtg ctgatttcca tgattaccgc agtcctcggc gcagtcatca accgcagact
    14221 gcaaaatgtg gatatgctgg atgcactgaa atccgtggaa taatccccgg aacaacttca
    14281 aaagacattc ccaccctcaa cagaaaagcg aaccattccc caaagaatgg ttcgcttttt
    14341 tctgtatctt ccgttttatc tatgcaattt gcaaatttta gcaattttat aggaaaatct
    14401 cttgaaaaat tgcggcgaat atgttacaat agaagtatca cagtgtgtgt ttgcagattt
    14461 gggacggaca cacccacaga atgaccaaag gagtccccca actatgaagt ggctgacaaa
    14521 acgacatatc gcactctatc tgacaatttt aatgctggcg ttcctggtgg ctgcatttgc
    14581 cattaccaag ggaacaccgc tccataaaga ggatacccac tatctctaca gcggctggct
    14641 cactgcggaa agcagagatc ccgaaaacga gccgtatttt gaaaaaggac agaccgtcta
    14701 tcagctccgg atggtgcatg aactggacga cacggtcaag ggaaaagtac tctctttccg
    14761 cacctatgat tcctacgtgg acgcctggtt ctgcgactca ccagacatcc cggcagacaa
    14821 cgacagcgcc ttttatcatt tcggcgaaac gctgcggttc tgcgattccc ccggcacgta
    14881 tacccatttt ctctccatcc cggaaacgga tgcacagtat cttgtcatac gggtggagac
    14941 tgcatatgcc aacaagttcc tcacgtccta tgacgtggca atcggcacgg aaaacgaact
    15001 gctgtacaac aatctgaaag aggaccttat ccccagtatc tccaatctgg gcatgatgat
    15061 tttcggcatt ctgctgatcg tcatttacat tatcggcagg tgcaagcgca ttgcctccgt
    15121 agaatccctt tccctgggct gtctctccat tgcgtttgcc gtcaacttca actgtcctct
    15181 gtttctgaac cagtatctct atcagaacgc cgtggtgcag tactacgcca actatttttc
    15241 cctgttcctg ctgccgctgc tggtgatcct gtactttgag gacattgtgc cgaaactgcg
    15301 catgagatgg atgttctacg gctttctgct cctggaggca gccctcagcg tggttcattt
    15361 caccggcatc gcatcctaca cacgaaccat caaatcattt accgccgcac tgggcattct
    15421 ggcagtggtc tccattcttt tgattatcaa gcactaccgg gaaatggagc ggttcaaccg
    15481 catcagcatc atactcctgc tcagctttgt ctgcggaaat gtgatcttct tcatttttgt
    15541 ctccaccctg ggcgaccaga cctttatcat ccgcaccggc gtactgctct acctggggct
    15601 tgctgtggtc aacggcatcc gcaagctgat gaatgagatc aaccgggaac gggaatcccg
    15661 gcttttgcag gagatcgcct acacagacaa gctcaccaaa atgggcaacc gctatgccct
    15721 ggaacgggat gccagagaat gcgtgctgga acagaccagc atcgtctcca tggatctgaa
    15781 cctgctgaaa accaccaacg acacctacgg ccacgccggc ggtgatgtgc tgctgcaaag
    15841 cgccgcaaag tgcatgactg ccgtctatga taaggtatac cgggtgggcg gcgacgagtt
    15901 caggaaacac tgattaaatc ttcccattac gcagcaaata tggtataata gaaaaa
//
LOCUS       DAWENO010000034        15627 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1575_length_15627_cov_10.258091, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000034 DAWENO010000000
VERSION     DAWENO010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15627
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_15627_cov_10.258091"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2830..2839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3721..3730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12305..12314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14579..14588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3700 a         4213 c         3904 g         3770 t
ORIGIN      
        1 gactctgaaa gcgaactgag aactgttaaa accggaaggt tgataaaata gaacaacata
       61 taagaaattg cggctaccac cattttttcg aagaagtttc cccgcagcaa tatggcagca
      121 taaataaaat tggtaagcac aaatccggcg accagaatta tctcattctg aatcagaaaa
      181 ctccccaagg catcaatgca gacgagtatc cccaccagga tgccccattt cagcagacgt
      241 ttttccggag aacgaaagcc gaaataccgg atcataagcc gtgccaccat aaagcattct
      301 gccaaaattg cgatccattc tatcactgtc cagatcatgt atcatcttct cctctcataa
      361 aatactgata ccgtttccgg actgccgcac ttctcctgtg gctcagcggc agttttttca
      421 agcggtgcgg cgtaagttct actgtgtcat tcccgatgtg gtagacatag cggtaattca
      481 gcagatacga cttgtggatg cgcaggaaac cagagcggct gaactgctct tccagctttt
      541 ccaatgtgta ctctctggcg ctgagttctg cggtctcctc cagcgtcacc agataaatgt
      601 gatggcgaat ggaaaagaaa tacacgacct ccaccagctt tttggtgacg atgcgcttgt
      661 cgtccagcac aagtctggcg gtctgtccgc ttttggcgta gttttcacag tacgccttta
      721 ccgcatccgg cagttcctcg gcgagccatt ccttccggag aaaccgaaac ggctgaaact
      781 gaaacgactc aaagacaaag tcgctgtgac tggaaacgaa aatcaaagct gccgcagaat
      841 tcatggctct gatctctgcg gcaagttcca tgccggacat ctccggcata tcaatatcca
      901 gaaacagcag ctggaattct tccttgcggt gcgcctggat cagctccggc gcattggtgt
      961 agacaaagca ttggaactcc atgccctgtt tttcgaacag cgcctccacc atgtcacgca
     1021 gacagcgcac aaaaataact tcatcatcac aaactgctat ccgtatcata cacattcgcc
     1081 tcccggtatc atcataccac aaattttgcc aaaaagcaag aaaaaacctg ccgccttgcc
     1141 aagccgccgg actcatggta taataggttg cagacaacga cactgaacag gagggtgttt
     1201 ccatgacata ccgcattttt ctggtggagg atgaccgcag cattgcagag ggacttcagc
     1261 accagctgga acaatggaac tttgaggttc gtgtggtgca gaattttcgt ggcgttctgg
     1321 gcgaattcac cgcattcgat ccccatctgg ttctgatgga cattatgctg ccctgctacg
     1381 acggctacca ctggtgccgg gagatacgcc gtgtctccga agttcccatc atctttctct
     1441 cttccgcatc ggacaacatg aacctcatca tggcaatgaa catgggcggt gacgatttca
     1501 tccccaagcc ctttgactgg aatgtcctgc tggcgaagat ccaggcgctg ctgcggcgca
     1561 cctatgactt cggcggacag gtctcactcc tggaacaccg ggatgccatt ctgaacaccg
     1621 ccgatgccgt cctcacctat cacggcgagc gcatcgatct gaccaaaaac gaataccgga
     1681 ttctccaggc gctcatggag cagaagggca aggtcgtcag ccgggagacg ctgatggaac
     1741 ggctctgggc aacggacagc tttgtagacg aaaacaccct caccgtcaac gtgaaccgcc
     1801 tgcggaaaaa actggacaag gcagggctgc acgactttat ccgcaccaaa gtcggcatgg
     1861 gatacctggt agaataggag ctgtgaccca tgaaaatatt ccggagtttt ttcaagctgc
     1921 atcagaacgg cattcttgca tttgtgctgt gcagcattgt gtttgttttg attttcttcc
     1981 tgtatgacct gccggtcggc gccgtgctgt acagcgccgt gctttccgcc gtcatctgtc
     2041 tggcgctgtt tctggcaagc tttatccgct attgcaagcg atgccgtatg ctccagaaaa
     2101 tgcagcagga gatcactgtg accatacagc atctccccca gccccaggac tgcaaagaac
     2161 aggcatacca ggaacttctg aaacgccttt ttcagaaaca gcaggaacag gcgcaggcgt
     2221 ggcaggtgcg gtgcagcgat atgatggagt actacactgc ctggacacat cagatgaaaa
     2281 cgcctatcac agccatgcag ctgcacttac aggcggagca ttcaccggaa aaccgtgcct
     2341 tgctggatga cttgcagcgc atcgaacagt atgtggaaat ggtgctgtgc tatctccgtc
     2401 tggattccga cttcacggac tatctcttcg ccaaatacga cctggacaat attgtccggc
     2461 aggcggtgcg ccgtttttcc tcccagttca tccaccggaa gattcgtctg gaatacgagc
     2521 cgctgaactg cgaggtgctg acagacgaaa aatggctgct gtttgtactg gaacaggtgt
     2581 tgtccaatgc gctgaaatac acaccctccg gcggcacgat ctccattttc ctggaatcgc
     2641 cgaaaacgct ctgcatccgg gacaccggca tcggcattgc cgcagaagat ctgccacgca
     2701 tttttgaaaa gggctttacc ggatgcaacg gcagaacgga caaaaaagcc agcggcatcg
     2761 gactgtatct gtgcaggcgt atctgcagaa atctgaacca cacgatcaca gcgcagtccc
     2821 agaccgaccn nnnnnnnnna cacgtttcgg cggaaaccag gtcgaggcac tgcggaatgt
     2881 ttcctttacg gtggaacagg gcgaatatac ggcgatcatg ggagaatccg gttcgggaaa
     2941 gaccaccctg ctgaacatcc tggcggctct ggataagccg accggcggca cagtcctgct
     3001 ggacggaaag gatttgacgc agatcaagga gtcacagatc gccgcatttc gccgggacaa
     3061 cctgggcttt gtgttccagg acttcaacct gctggacacc ttttccctgg aggacaacat
     3121 ctatctgccg ctggtgctga ccggaacgcc ttatgcggag atgcagcgcc ggctcgcacc
     3181 cattgcgcag cagctgggca ttacagaact gctgcaaaaa tttccctatg aggtatccgg
     3241 cggtcagaaa cagcgtgccg ccgtggcacg ggcgcttatc accaacccac gtctgatcct
     3301 ggcagacgaa ccgaccggcg cattggattc ccgttccaca gacgaactgc tgtccctgtt
     3361 ttctgccatt caccaatccg ggcagaccat cgtcatggtg acccactcca ccaaggcggc
     3421 atccagcgcc ggacgtgtca tgttcatcaa ggacggcgag gtgttccacc agctgtaccg
     3481 gggcaacgat accaaccagc agttctacca gcggatttcc gacaccctta ccctgctgac
     3541 gaccggaggt gaggttcgat gaaaatgaat ctgtatccga aactggcatg gcacggcatc
     3601 agcaaaaaca agaaaaccta tgtgccgttc ctgttgacca gcatcggtct ggtcatgatg
     3661 ttttacatcg tgtcctatct cacctacaac aaatccgtca agcagatgcg tggcggcgac
     3721 nnnnnnnnnn gatcctgtcc tggggcgtgc ctgtggtggc atttttcgtg gtgatctttc
     3781 tgttttacat gaattccttt ctgatgcgcc gccggaaaac cgagttcgga ctttacaaca
     3841 tccttggcat gggaaaaagc aacatcgccc gtattcttct gtggcagaat ttcatgctgc
     3901 tcgttgtctc cgcagcaggc ggtctgggca tgggtatcct gctttctaag ctggcagaac
     3961 tctgtgcggc aaaaatgctg tccaatcaag cctctctggt atttgtcatt gaaccctatg
     4021 cggtgcgaaa cacactgatt ctgactgtgc tgtcctttgt gctgatcctg ctgtattccc
     4081 tgggacagat ccatgtggca aagccggtgg aactgctcca cggggaaaag accggcgaaa
     4141 agccgccgaa ggcacgctgg atactggcac tgatcggcgt ggtacttctg ggtaccgcat
     4201 attacctggc agtgaccacc agagaaccgg tacaggcgct gctggtgctg ttcgtggcga
     4261 tcgttctggt catcatcggt tcctacctgc tgttcatctg cggctctgtg gcgctgtgca
     4321 agatgctcca gaaaaacaaa aagtattact ataagctgaa ccatttcatt tccgtttcct
     4381 ccatgtccta tcgcatgaaa cgcaacggcg caagcctggc atccatctgc atcctctcca
     4441 ccggcgtgct ggtgatgatc tcctcttccc tctgcctgcg catcggcgag gatgacagcc
     4501 tgcacacacg ctatcccaga gatatcgaag tcggcattct caccaaggac tatgaccaga
     4561 gtatggaggc tgcctcgtac attgaacagc aggtggatgc cgtattgcag gcatcacacc
     4621 agaccagaaa aaacgaactc gcctacaaca atttgaattt cgcggcgtta aggcagggcg
     4681 gcgacatttc ggtaaaacag tacacagaag tcagcggcac tcagtatgcg gagagcatcg
     4741 acaaactggt catgctgaat ttcgtgacac tggacaccta tgaaaaaatc accggcacaa
     4801 cggaaacact gggcgaaaac gaagtgctgt tgtgtatgtt ccgcggaacg ttcagcggag
     4861 attccctgaa tatcgatgac cagttccaat ttcatatcag gaagcaggtc cctgactttc
     4921 ttgaaagcgg cgacaatgcc attgcgacct atccgaccat gtacctggtg gttccggacg
     4981 gtgaaaccat gagccggctc tatgagtatc agtattctgt ctacggcgaa gaaatggcgt
     5041 ccagctatca gacctggctg ggatttgatc tggactgcaa tgccgatacc cagatcgaac
     5101 tgaccaaaca gctgtacgac cagtttgcgg aaggttctga aactcatccg aacttctatt
     5161 atgagatcaa aggcttggag acacagcggt acagtttcta ttccctgttc ggcggactgc
     5221 tgtttctggg aatcctgctg agcgtggtat tcctgttcgg cacggtgctg attatgtact
     5281 acaagcagat ctccgagggc tatgaagatg cggcacgttt cgagatcctg caaaaagtgg
     5341 gcatgactcg aaaagagatc aaaaagagca tcaattctca gatactgacc gtctttgcgg
     5401 cgccgctgct ggcatccggc gtacatcttt gctttgcatt tccgctgatc caaaagctgc
     5461 tgctcatgtt cggcttatcc aatacaccgc tgctggtggg cgtgactgtg ggcagctatc
     5521 tgatctttgg cgtgctgtat gccgtgatgt acaaggcgac ctcccgcagc tattaccagc
     5581 tggtgaaata atgcggcagc gaatcgtgct tgctctggga ctgaccctgc tggcaatcgt
     5641 ggctgtaccg ctggcagtgt tcctgggcat tgccggactg atctggaaaa tcatgaactg
     5701 gctgctggaa aggagcgatg tccaatgaaa tgcagcaaaa tcgcagcact gctgctcttg
     5761 ccggtcctgc tgtgtaccgc cggatgcagt gtgaaaagcg gcatcctcag cggctatgaa
     5821 aaggtgatct ccgccatggg gacgctgaca cccacaccgg atgctgcgct gatcggcgtg
     5881 cgcacgcccg gcagagacga ctgtaccggc gcatatgccg cctcctgtga aaaggcacgg
     5941 ggcaaggatg tggtctttgg cggatgcggt ttgcagacac gccggctgga gatcacgggt
     6001 tctgtctcct gtcaagccgg atttgtacaa attctgctga aaaacggcag caaggagatc
     6061 cggctgacgc ccaacagcag cggcaatatt gcagaaagca tctgcggcgc cggcggcgac
     6121 tggtatctgt ctgtggaata tgcagacttt tccggcagcg tgacactttg cagcagagac
     6181 acaacacaat aaaccataca aaaagcgtac caatgtatat ggtacgcttt tttcgatact
     6241 gtacttttta ttctgcactt ttcatcttcc ggcggtactc cgtgggtgaa catccggcga
     6301 ctttgcggaa ctgccggttg aagttggaga tattccggaa tccgcagtcg aaagcaattt
     6361 ccaggacgct ctcttttgtg ctggtcagac gcttgcacgc ctggttgatg cggaagtggg
     6421 aaatgtattc tatggcgctg aatcccacat atttctgaaa ctggttcata aagtagcttt
     6481 cgctcagatg caccaccgcc gcaagctggc gtatcgtgat gttctcccgg aaatggatgc
     6541 tgatatattc cagtgccggt cgaatcactt cgcttggctc atgaaaattc gcatccactt
     6601 ccgactccgt ttccagcagc cagaagaacc ggagcaattc gctgcgcatc agcatatcca
     6661 gctgtggcgc atcccctttg gcgcaggcaa agatgttctc catcatcatt tccagttcgt
     6721 gatagtagtg atgcgctgcg gtaatgtgca tgggaatccg catactgccg ttcatcagcg
     6781 gcttaatgca ctcgttgata tagcggtccg tttccgagcg cccgaagatc tcgtggctga
     6841 acaccagcgt gtcatagacc tggtgcgcat cgggacaggg atagaccgag tgcatcacat
     6901 ttggctgtat gacaatgata tcccctgccg aagagacaaa cttttcgtct ccgcagttga
     6961 attcggcaaa tccctcccgg acgaagttga tctcgaattc gctgtgccag tgcatgggcg
     7021 cataggcaaa ttcgtccggg atcatacact gataatagct gaacggcttg acctttgagc
     7081 tatgcacccg attttctctg taattcatat cgtctcctcc aataaaatag gatcgtatca
     7141 gaatatcgta gaataacaat agatttttgt gcttttttat tgtattatat catatagaaa
     7201 gaaaaaacgc aatgaccttt tgaaaggaag tgctgttatg attcaaaaat atgtatacgg
     7261 acacccattc cccaccgacg ctgtggtaaa ggagatcgag accgccaaag aacccctgcc
     7321 gtttttcgaa acggacaacc agggcagttt tacctatacg cttgcggaag acgacattgt
     7381 atacggtctg ggcgaacaga tccggggcat caacaagcgt ggctggcagt atgtcagctg
     7441 gaactatgac aaccccaacc accacgagga cacccgttcg ctctacggct cgcacaattt
     7501 tatcattgtc tgcggcaagg tcactttcgg tgcatttttc gattatcccg gaaagatgga
     7561 gttcgacatc ggctataccc ggagagacac catgcagatc aaggcggcga aaaatgacct
     7621 caccgtctat atcatcaccg gtgaaaacga aaaggatatc gtcaagcagt tccgtgggat
     7681 catcggcaga agttatattc cgccgctctg ggcattcggc tacggacaga gccgctgggg
     7741 ttataaaaat gaggcagata tccgggaagt cgctgcaaag tacaaggctg ccggcattcc
     7801 cctggacagc atctatctgg acatcgacta catggagcgc tacaaggact tcaccgtgga
     7861 tgaagagcgt ttcccggatc tgaagggact ggctgccgat atgcaggcag agggcatcca
     7921 tctggttccc atcattgatg ccggtgtcaa gatcgaggac ggctacagcg tttatgaaga
     7981 gggcgtggag aaaaattact tctgcaaaaa tgcagaaggc ggcgattttg tgggcgcagt
     8041 atggccgggt cgggtacact tcccggattt cctccagccg gaggcaagag actggttcgg
     8101 caaaaaatat gccgttctga cagaacaggg cattgacggt ttctggaacg acatgaacga
     8161 accggcaatt ttctacaccg aagaccgtct ggcagacact tgtgccgaga tcgaaaagct
     8221 gactgccgga aacatgggca ttgacgaata ctttgccttc accggaatgg tagccggact
     8281 gaacggcaac attggcgact atgacaagtt ctatcataac gtcaacggtc agatggtcaa
     8341 gcacagcgag gtacacaacc tgtacggcat gaacatgaca cgctctgcaa atgaggcact
     8401 gcgtgagatc tgtccggaca agcgtaccct gttcttctcc cgttcttcct atgtgggtgc
     8461 gcaccgctac ggcggcatct ggcagggcga caacaagtcc tggtggtcgc acattctcca
     8521 gtccatgcag cagctgccgg cgctgaacat ggcaggcttc ctcttcaccg gcagcgatac
     8581 cggcggattc ggctgtgaca ccacagaaga tctgatgctc cgctggctgc agtactccct
     8641 gtttacgccc ctgttccgca accactcggc tgacggcaca cgagaacagg aactctatcg
     8701 tttcagcaac gcagacgctg ccggaaacat gatccgcatc cgctacagcc tggttccgta
     8761 tctctacagc gaattcttaa aggctgccct gcaggatgag atgatgttcc gtccgctcgc
     8821 cttcgacttt gcagacgatg cggatgccaa gcaggtggac gatcagctcc ttctgggcaa
     8881 tgagctgatg atcgcaccca tctacaagca gaatgcaaag ggcagatatg tttacctgcc
     8941 ggaagagatg atgctggtca gaatgcgttc cgacgaagat tacgagacag agatcctgcc
     9001 gaagggtcac cactatgttc cggcagagct gaacgaactg gtattcttta tccggaatca
     9061 aaaggcgatt ccttttgcaa aggcggcaaa gaacaccgca gaaaccgact ggaacacagt
     9121 ccgtctcctg ggctattccg gatgcagcta tgagatgtac gcagacaacg gcacttcccc
     9181 gaatccggag gacagtctga ccgtttctgt catagaaaag taaaagtcca aaaggcatac
     9241 gacacaaaag gcagctggga aaaaatcccg gctgcctttt gcgttttggt gaaaacgacg
     9301 atcagataag gatgccctca aagtgatcca tttcgtgctg aatgatctgc gcctcaaaat
     9361 cccggaatgt ctgctttttc cgtttgaact ttcgatccag atattctacc acgatcacac
     9421 tgtaccgttt gaccggacgc tgtcccgtca gcgacagaca gctttcttcg gtctcgtagc
     9481 actgtttgga atgatcgacg atcttcggat ttatcatgac gagatagtct ttcccgatct
     9541 gtgccgccag aatggtcttc cgctccccga tcatattcgc cgccatccct acacagcggt
     9601 cggcgttcgc acaaatggta tccaacaggt cctgtaccac gggcagatcc tgtctggttg
     9661 ccagctcgga tttctggctg aggaaaaatg tgtcttttac aatttcacga atcatcttgg
     9721 catctcctac tggttgattt tatctctatt ataccacacc ttttgcaaat acgcaaacaa
     9781 aatgctgctg cacccataca acaggtacag cagcattttt ccgatttgaa cggattccgt
     9841 tttgccttat tttcgcagat ccacattccg cacagcgtcc cgtactttca gcacgaacgc
     9901 cggagaaacg gaaagttcgt caatgcccat tcgcaggaat gtttccgtca gcgtggtatc
     9961 cgccgccagt tcgccgcaga taccgatcca cgcaccgttg gcgtgggcat tcttggcact
    10021 catttcgatg agccgcagaa tcgcctcgtg gtgggtgtcg ataaacggtt cgatgtctgg
    10081 attctgccgg tcgcaggcaa gggtgtactg ggtcagatcg tttgtgccaa cactgaaaaa
    10141 gtccaccatg ggtgccagac ggtcgctgat aatggcagct gccggcgttt cgatcatgat
    10201 gcccagttcc atggtgtcgg aataggtcac accctgctcc cgcagttccg ccttgacctc
    10261 ctcacagatg gcaagaatct tttccagttc ggatacgctg gtaatcatgg ggaacatgac
    10321 gcccaggttg ccgtagatag atgcccggta cagcgcccgg agctgcgtct tgaagatctc
    10381 cggtctggtc agacaaatgc gaatggcacg gtagcccatt gccggattgt cctctttttt
    10441 caagtggaag taatccacct gcttgtctgc accgatgtcc agcgtgcgga tgatgacttt
    10501 cttccctgcc atactctcca gcacacgctt atacgccagg aactgctgtt cctcattggg
    10561 atagtccgta ttttccagat acaggaattc actccggaac agtccgatac caccggcgtc
    10621 gttaagcagc actgcaccga tgttgtccac actgccgata ttggcataga tgttgacctt
    10681 ggtgccgtcc ttggtgatgt tttccttgcc tttcagttcc agcagcagtc gctttttctc
    10741 ctcgtctgca cgctgctttt ccagcagacg ctggcgagtc tcctcgtccg gttccacgaa
    10801 aatttcaccg gtaaagccgt ctacaattgc ctcggtgccg tcctgcacct ctttcagaaa
    10861 atcgctgccc acaccgatga ctgccggaat gttcatgttc cgggcaagaa tcgccgtatg
    10921 ggagttggac gagccgtgtg ccgtcacaaa cgccagcacc ttgtccttat ccagagaaac
    10981 cgtttcgctg ggtgcaaggt cgtcggcaca gataatgcgc ttttcgtcgg aatcctcctg
    11041 cacggcaaca ttccccgtca gattggcaat gatgcggtcg gagatgtccc gcacgtcagc
    11101 cgcacgtgcc tgcatatagg catcgtccat ggcggaaaac atctcggcga agttgtccgc
    11161 cgtaatggaa accgcatact cggcgttgac cttctgggtg tcgatgatgt tttggatgct
    11221 ctcgttgtag tcgtcgtcct ccaacatcat catgtgaatt tcaaagatct gggcgttggt
    11281 ttcgcccacc tctttcagcg ccttttcata gatcgcctgg agttgttccg atgccgcagc
    11341 ctttgccgcc tctactctgg ctttttccgc ctcggtatct gtgacagagg tgcgctggat
    11401 caccgtatcc tgctttttga acagcgaaat ttttcccatt gcaacagcgc cgtacacgcc
    11461 ttttccggta aattgtttca tgattccgat tcctccttac gcctctgccg taccgaactt
    11521 tttccggtag ttttccattg ccttttccat gactgccact gccgccagcg gcccgccgat
    11581 gtccttgact tcggtctgct tgccccgttc cagctgctta tacaccgaga aaaagtgcat
    11641 cagctcctcg aagatatgct tgggcagctc actgacatcg gtataactca tataggtggg
    11701 atcgccgtag ggaatggcaa tgatcttctc gtcgcccata ccgccgtcct ccatggtcat
    11761 gacaccgatg ggatagctgc gcaccaatgt cataggcagg atcggttcgg agcagagcag
    11821 cagcacgtcc agcgggtcat ggtcgtctgc ataggttctg gggataaaac cgtagttcat
    11881 ggggtagtgt gttgccgtga acagcactct gtccagggac agcataccgg ttttcttgtc
    11941 cagttcatac ttcatattgc tgcccatgga gatttccaca acagccataa aatctgtggg
    12001 ataaatgcgt tcttcgtcaa tgtcatgcca gatattcatg gattttccct cctatatgaa
    12061 tgctttttcg agaatcagag gttctgctgg aagaatgcct gcatctctgc gaaagcggtc
    12121 tcttcgtctg cgccgtcaac agaaacggtc acggtgtcgc cgcacttcac gcccatgccc
    12181 atgaccgcca tcagcttggt caggttcaca gacttgccgc ctttttccat ggtaatggtg
    12241 ctctggaatt cctttgcctt tttcgccagc agtcctgccg gtcttgcgtg gatacctact
    12301 tcgtnnnnnn nnnntttcat gtgtaattcc tcctaaataa attttatttt aacatctgca
    12361 aaagcagtgt tggtttactt tgtgatttga attgccgctg ttccggcaga aaccgttccc
    12421 tgtgcgactg gtgtgattgc cgcataatca tctgcattgc acaccagcag cggcgtagtc
    12481 agcttatagc ctgcggcggt gattgcctcc atgtcaaagc ttaccagcaa gtcgcccttt
    12541 ttgacctgct gtccgtctgt gacgtgcgcc tcgaaatact tgccctccag cttgacggta
    12601 tcaatgccaa tgtgcatcag cagctctgca ccgtccgctg aggtcatgtt tacagcgtgc
    12661 ttggtctcaa agacggtctc gatcgtgccg tcacaaggtg catacagctt gccctccgac
    12721 ggacggactg caacgccgtc gcccagaatc ttctgggaga acacctcgtc ctccacttct
    12781 tccagcggaa ctgccgtgcc gttcatgtgt gcgaaaagtg taatcgtctc tgcggcactg
    12841 ccctgttccg gcgcagtgtt cttctgtacc ggttccggct gtttttcctc tgccggcgtt
    12901 ttttctgctg tctcctggaa aaggctgtcc agatcatccg attccggcgt atccaggaag
    12961 ttttccagat tggatttgat aacggatacc tttggcccgt agatgacctg tacgccgttg
    13021 cccttgtgaa tcacaccgga tgcgccgctg gctttcagca tagcatcatc gaccagtgcg
    13081 gcatcttcta cggtcacacg cagacgagtt gcacagcagt ccagatcgga caggttcgcc
    13141 ttgccgccca gacctttcag caccatggcg ctgaccatat ccactgcggc tgcggcggca
    13201 gactttttct ctgcctttgc cgcattgaca tcacttctgc ggtagagttt cggttctaca
    13261 tcatccggtt ctctgcccgg tgttttcaga tttagtttgg taatcataaa gtagaatacg
    13321 aaatagtaga ccacagcgta gatcagtccc acaaccacga tccagatcca atgggttttc
    13381 gcattgccct ggagaatgcc gaacagtgtc aggtcaatgg caccgccgga gaatgtcata
    13441 ccgacaccca cattcagaat gtgcatcagc atataggaca gaccagccag cacgcagtgt
    13501 acagcataca tcagcggagc cacaaacagg aaggtgaatt ccagcggctc ggtgatgccg
    13561 gtaatcatag aagtcagtgc tgcggagagc agcaggcttc caaccacctt tttcttttcc
    13621 ggacgagccg cacggtacat ggcaaatgcc gcagccggca gaccgaagat catgaacggg
    13681 aacttaccgg acataaagcg tgttgcagaa acggaaaatt ctgtggtaga cttggatgcc
    13741 agttctgcga agaagatgtt ctgcgcaccg gaaaccatgt tgccgtcgat catcatggaa
    13801 ccgcccagtt ccgtctgcca gaacggcata tagaacacgt gatgcagtcc gaacgggatc
    13861 agcgcacgtt ccagaatacc atagatccat gtaccggcgt aaccggagtg aagaaccagt
    13921 gtacccagat gcgagatgcc ggactgtacg atcggccaga gatagaacat gacaacgccc
    13981 acgatcagat aaaccacggt agaaatgatc gggacaaagc gtgtgccgcc gaagaaggac
    14041 agcacctggg gcagttcaat tttgtagaac cggttgtgca gtgcagcaac gcccagtccc
    14101 accaggatac cgccgaacac acccatttgc agtgtggtga tgcccagcac ggaagtggtg
    14161 aaattcgctg ccattgcctc aacgccgcct ttggcggaga tcagcgcact gatggcagtg
    14221 ttcatgacca ggtaagcaat tgcgccggaa agcgctgcaa cttctttttc ctttttcgcc
    14281 atgccgatgg aaacgcccat ggcaaacaac agcgccaggt tatcgaacac ggcactgcct
    14341 gccttgctca tgatatccag aatggtgtag agcagtccgc cctcgtaaat gatattgccc
    14401 aaatgatagg tctcaatggt cgtggcgttg gtaaaggaac ttcccagacc cagcagaagt
    14461 ccagccacgg gcagcagcgc aatggggagc ataaacgagc gccccactcg ctgcaatacg
    14521 ccgaaaatct tgtctttcat tacaaaagtc ctcctaaaaa tgttctgtat ctttacgcnn
    14581 nnnnnnnnac cagttgcaat gaaatcaagc gtttttcaac gggactcgct gatctttttc
    14641 agatgaatgg tcagcgtcat catctcatct tcggagatgg tccgtgcaaa gtttttcaga
    14701 atgtagtcct gtatgcactt tgcacagcgg taatgctttt ggaatttcgc ccggatgaaa
    14761 tccagtatct cttcatcccg gctcagtaca ttggggaatt cctgggactg aaacagccgt
    14821 ttcgccagat atttcagatg caccagaaag cgttcataag aaaccgtggt cttgtccagt
    14881 ttcccacgat agaaagtatc tgcaatttct gcacagccat tcaccagctt tgtaatatcc
    14941 ggtacttgtc ccatattggt atgcagccgt gcattgataa tgtgcatcgc caggaatcct
    15001 gcctcgtcct catggagcga aacgcccagc tgctgccgga tctcttccag gcagtatctt
    15061 ccgagtgcaa gctcctccgg gaaataatcg tgaatggaat ccatcagcgg attggtaaat
    15121 gtcatgcctt gtttctgccg ctcaatggca aaactgatat gatctgacat actgataatc
    15181 agagactctt tcagcacttc ctggtgcagc tgctctgcaa tgtggtctac caggtcggaa
    15241 gtcagtcgga tgtgttcata cgggatctcc gcaagacact ccaccattcg tttccgcagc
    15301 gccgaatcgg taatcagata aatcttctgc acctgcgccg ggtcgatctc gtccccacgc
    15361 ttttttccga atccgatgcc ttttccggaa acgatctgtt ctctgccttt tttatccaat
    15421 gtgcaaacgg tattgttgtt gataactttt ataattttca taaagcacct cgaaaatggt
    15481 ataaaaaaag ccaaccccta taccgctatc attttgcgtg cataaaagtt ggttatgccc
    15541 aatcagacaa aattgtcgaa aggtaacatt ccaatatttc aattctcgaa acgttgtatc
    15601 aaatttacta taatcagttt atcatgt
//
LOCUS       DAWENO010000035        15304 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1609_length_15304_cov_11.966883, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000035 DAWENO010000000
VERSION     DAWENO010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_15304_cov_11.966883"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    888..897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3959 a         3721 c         4273 g         3341 t
ORIGIN      
        1 gcgtgcggca accatcgcag gacttgccgg cgtgaccatt ccggaggaaa cacgtgtcct
       61 catcggtgaa gtcacttctg tggacatcag cgaagagttc gcacacgaaa agctctctcc
      121 tgtactggca atgtaccgtt cggagaactt cgagcaggca gtggaatatg cggaccagct
      181 ggtcaaggac ggcggctatg gccatacagc atccctctat gtggatgagg tcaaccactg
      241 ggaaaagatc gaacagtttg cggcacggat gaagacctgc cgtatcgtaa tcaacacacc
      301 gtcctctcac ggcggtatcg gtgatctgta caactttaag cttgcgccgt ctctgacgct
      361 gggctgcggt tcctggggcg gaaactctgt ctcggaaaac gtaggcgtaa agcatctgct
      421 gaacatcaaa accgttgctg agaggagaga aaatatgctg tggttccgca caccgcagaa
      481 ggtgtacttc aaaaagggct gcctgccggt tgccctccgt gagatccagg atgtgctgca
      541 ccggaaacgg gccttcctgg taacagatga attcctgttc aagagcggca agatgagctg
      601 catcacagag ccgctgcagc agatgggaat tccgtatcag gtattctcgg acgtacagtc
      661 tgacccgacc ctggcgtgtg cgaaggaagg cgcagagctg atgcggacat tccagccgga
      721 tgtgatcatt gctctgggcg gcggctcggc aatggatgcc gcaaagatca tgtgggtgct
      781 gtatgagcat ccggacgcag atttcgccga tatggcaatg cgtttcctgg atatccgcaa
      841 gcgcacctat gagttcccgg agatgggcga aaaggcatac tttatcgnnn nnnnnnnatc
      901 gctgtgccga cctcttccgg tactggttcg gaggtaacgc ctttcgcagt tatcaccgac
      961 gaatccaccg gcgcaaagta tccgctggca gactatcagc tgctgccgaa tatggcgatc
     1021 atcgacaccg atctgatgat gtcccagcct cgtggtctga cctcggcatc cggtattgac
     1081 gcaatgaccc acgctctgga ggcatacgct tctgtgatgg cgaccgactt caccgacggt
     1141 ctggcactga aggcgctgaa gaacatcttc acctatctgc cggaatgcta tgagaacggc
     1201 gaacacgcac cgctggcacg ggaaaagatg gcgaacgcat ccacaatggc tggtatggca
     1261 tttgcaaacg cattcctggg cgtgtgccac tctatggcgc ataagctggg tgcattccac
     1321 cacctgcctc acggcgtggc aaatgcgatc ctcattacgg atatcatgcg ctacaacatc
     1381 gcagaggcac cccagaagat gggaaccttc tcgcagtatc cgtaccccaa tgcactgcct
     1441 cgctactgcg aatgcgcacg gtttgtgggc gtgaccggcg atacagacga agaagtattc
     1501 gaaaacttca ttgtcaagat cgaagaactc aaggcacgtg tgggcatcaa aaagaccatc
     1561 cgtgactacg gcgtaacgga agaggacttc ctggcaacgc tggacgaaat gtgcgagcag
     1621 gcatttgacg accagtgcac cggtgcaaat ccccggtatc cgctgatctc ggagatcaag
     1681 gcaatgtacc tcaaggcatt ttacggcgag accccggcag aggagaaaga ctcggctgag
     1741 aaaggagcgt aaacaatatg gaaccgttgt tggaaggact ggaaaaaacc agctatcatg
     1801 aaagaaatac ccgtgtcaag gtgtttggac acggctttaa gaaatcggat atcctcaacg
     1861 aggcactgaa ccaggctcgt gtggaggaga ccggtctgcg catcccgaag ctgctggagg
     1921 tgcggaaggt agacggcaag tgggcaatcg tcactgaatt cgtcgagggc gagaccctgc
     1981 agtccctgat ggagcagcat cccgaccagc tggaaagcta tatggaacag tttgttctgc
     2041 tgcaaaagga gatccagagc aagcgttcgc cgctgctgac caagctgaaa gacaagatga
     2101 accggaagat cagccagtcc ggactggacg ctaccacacg gtatgacctg cacacccggc
     2161 tgaacgccat gcacgaccat atcaaggtgt gccacgggga ctttaacccc agcaatgtca
     2221 ttgtcgacaa ggacggtaga ctgtatgtga tcgactggtc tcatgccaca cagggcaacg
     2281 gcagcgccga cgcagccaga acctacctgc tgttcaagct ggacggcaag gacgactggg
     2341 cggagctgta tctgaaaatg ttctgcgaca tgaacgacgt ggcaaagcag tatgtttctc
     2401 agtggctgcc cattgttgca gcatctcagc tggtaaagca gaatccccag gaaaaggaat
     2461 tcctgatgcg ctgggtcagc gtgtttgact atcagtaacg acaaaagcga gcaatcgcac
     2521 agatagaaaa aggcggacgg ggtgaacctg tccgcttttt gtgtgcaaag agtaacttca
     2581 aaaagtttcc cttgaaaagg gggatgtcag ctttagctga cagggggatt aacccctcag
     2641 tcaagcctac ggcttgccag ctcccctgtt aggggagcct acacattaca gagtatccat
     2701 acagagtatc catctcagca tacagcacac tttgcgatgg atttttcaag tgagataatt
     2761 aaaaattgtt gtggtcgtgt gcctgccctc tgtacaacgc cgcaaaaata tggtatacta
     2821 tctatacaac actgcacaaa aactcgaagg aggtcaaccc atgacaaacg agccctttac
     2881 cgaggagacg accggacaga tcgacccgca gaatcccttt gacaatgcgc tggagaattc
     2941 ggcataccgg acggattacc gccggtttct gccgcagatt ttgctgcccg gctttcagat
     3001 acccctgttc ccggagcgtg acgagcgccg gaacattcgc cgccagatga acaccgccgg
     3061 cgcagggatg ctgctgggga cgcttatcgc acagctgctg tttccgctgg cagtgctttt
     3121 gctgctgctc tgcatggacg gcacaacagc atcctatttc aacggcgacc agtccggcgc
     3181 agtccgcacc atcatgaaca gcggcatcta catggcactg cactgtctca ttctggcagg
     3241 ctctaacgtg cttgtcgctt tgctgggctg ccgcaggatt cgccagcctc tgggcggact
     3301 gttccgcacc acggattttt ccgggtggaa agcattccag tacatctgca tcggcatcgg
     3361 tctgcagtac gttgccgtgc tgatctacgc cgtgctgaaa tatgtggggc aggggctggg
     3421 gatggagttc ccggaagtgt ccttcgacta tttccagacc ggacagtcca ccatgctggt
     3481 actgctttac acctgcattc ttgcgccgat cacggaggaa ctgctgttcc ggggctttct
     3541 gctgaaaagt ctgtcagtgg tcagcacccg gttcggcgtc attgtgacgg cactgctgtt
     3601 cggactgatg cacggcaatg tccagcaggg catcctgggc gtgctggtgg ggctgtttct
     3661 ggggaaaatt gtgattcgcc acaactcgct gacaccgtcc attctggcgc atattgcgct
     3721 gaacaccagt tctactctta tcagcgtact gctggcactg ctgccggaaa acatcgggaa
     3781 cgtggcgatt ttgccgctgg aattcgtggt gctggcgatg gcggtcatgg gcgttgcgtt
     3841 ctggttcggg aaagagcgca aggaacccct gccgtatccc acccagaaac aggcgacccg
     3901 aaaccgtgtg ttctggtcgt cgccgctgct cctgctggca tttgccgcac tgctggggct
     3961 gatggtgtat catgcaatgg cggtgtgatt atttccgtgc cgactccttc gccacaactc
     4021 ctccgccctt cgggcacctc ctctatgaga ggaggcttaa tttgtctccc ctttagggga
     4081 gatgtcccga agggacagag gggttgagct ggcagccgtg aggatgactg aggagtttat
     4141 agtttttggt aaggtaaacc atgtaggggt cgatgcctac atcgacccga gcgtacagaa
     4201 aaggaaacgg cgaaaccgta ggggcgaaca ttgttcgccc gtgccgactc cttcgccaca
     4261 actcctccgc ccttcgggca cctcctctat gagaggaggc ttaatttgtc tcccctttag
     4321 gggagatgtc ccgaagggac agaggggttg agctggcagc cgtgaggctg actgaggagt
     4381 ttgcattgta aaagcaacat tctccctatg tgtaatccat tacacgtaat ttgtaaaatg
     4441 tgtctgaaat tgcaattaaa atgtgcggat aattcttcga cataatgcgc cgtcgacaaa
     4501 tcgaccgtat ttcagaaaac agatggattc ccacagatta tatgcggttt ggacacaact
     4561 ttatgacgat cgtcgatgcg ccatcagaat tgtgcagaaa atggaaaaag atggcagtca
     4621 aaaaggacac atttgcgtga tttttcttcc aatttgtgat tatgcacaaa aagtgaaata
     4681 aaatgttgta gtaaaagcgg aaactgcaca aagtgtattg acaagtgcgc cagattgtgc
     4741 tataataaat ttgcaatcaa cgagagggaa cgttgataag aactctcgtt tgaacttccc
     4801 ggtttctccg gatgcgacgc cggctaaccg caaaacggat gaaaactgag aacattgtta
     4861 ttttatttta ctttataaaa ttgaaaggtg gaaaccaata tgaaaacccc gatgaacatt
     4921 tataacactg caatgaagaa gaaaaagaac cgcaagggct tctccctggt agaactgatc
     4981 gttgttctgg taattatggc aatcctggtc gctgcactgg ttccgtcctt ggttggttat
     5041 attcgtcagg caagacaatc aaattctaag gatgaatgtg catctgctgt gcaagctgca
     5101 cagacaattg cctcgtccgc atatgcgtcg agcgataaaa aatatgtaaa caatacatcc
     5161 ggtagtggtg atgtgacatt tactactttt tctggtgatg tgagtgctgc aactaattat
     5221 ctgtcagcca cattgacact tgcagaactg aatggctcgg gaagtgctgc tattacaaaa
     5281 attgaattag gtagtggtgc aagtgagggc atcgttacta agcttgtgat gacagcttca
     5341 aatggtgata ctgttactta tgatacatct gcatctccta agtatagtgt aagtgcatct
     5401 taacttgatt ttcccatcta acatctaaaa caatacaaag cattcagcac attgggtgct
     5461 gggtgctttg tttggtttct gggcttttgg cgttctgtcg gttgtttttc acttttgtga
     5521 attgtttgtg gtgaaagaga ggagataccg catgactcct cagtcagcct tacggctgcc
     5581 agctcctctg gaagaggagc caaatagcct cctctcatag aggaggtgcc cgaagggcgg
     5641 aggagttggc aggagtcggc gagcggaaaa ataagcaaaa gtattttctt ttctgtacac
     5701 ttgggtcgat taggcatcga cccctacata attcgcctta ccaaaaacta taaacttctc
     5761 agtcagcctt acggctgcca gctcctctgg aagaggagcc aaatggcgga ggagtcggta
     5821 ggagtcggcg agcaaaaagt cagagaaagg acaaaacaat ggaaaacgaa cgaccaccga
     5881 tgtataccgg atataatcgt gcgttaaaag aaaaagccag agaactgcga aaaaatatga
     5941 caaaacaaga gcgccatttg tggtacgatt ttttagggta ccgacaggaa aattggaaac
     6001 gccagcgggc gattgatggc tatattgtcg attttttctg ctactcggcg aggctggtaa
     6061 tcgaactgga cggctcgcag cattatacag aagagggcga ggagtatgat ggtatccgta
     6121 ctgaggttct ggaacgatat cagctagaag ttatgcgttt tttgaattct gaggttgacc
     6181 ggaattttga ttatgtttgt cttgcaatag aggaaaaggt gcgtgaaaga actgggagtc
     6241 ctttctaaac atcgactccg ccgccaagac tcctccgccc tatcgggcac ctcctctata
     6301 agaggaggcg atttggctcc cctttagggc gcccgcaggg tgtgccttgt gctgagctgt
     6361 caccgtaggt gactgagggg ttgaacgggc ggatgtgggc atctgcccct acagtggcgg
     6421 cgtgcaaaac gcttgaaaat ataacccaat cgccaggaca cgacaagagg tgaataccgt
     6481 gccaaattac aaataccgtg ccacagccat gagcggaaag accgtgcgtg gcaatctggt
     6541 ggcatccgac ccggaggcac tgctgaacga actgaaaaaa caggacctgt tcctgctgga
     6601 ctatacccag accaacaaaa cttcgttcgg gtcgaaactg aaacccaagg aactgaccga
     6661 tttctgccgc cagctgggca gcctgctgga tgccggcgtt tcggtgatcc agtctttcaa
     6721 cattatttcc aaccgtgcca gcatctccaa attcgcccga aagtcctttt cggatatcac
     6781 cacggatctg aaacacggca aggcgctttc cgaggcgatg gcagaccagg gcaaggtctt
     6841 tcccgaactg ctcatcagca tggtgcgtgc cggcgaggcg agcggtaaga tcggcgatac
     6901 ctttctcagc atgggacagc actttcagaa acaacagcgc atcaagtctc aggtcaaggg
     6961 cgctctggcg taccccatcg tgctgatgat cctgctggtg ctggtcatta tcgtgatgtt
     7021 tacgtttatc ctgcccatgt tcggcgatat gttcacggac atggaactgc cgcttatgac
     7081 aagggttctg atgggcataa gcaatttcct cacccattac tggtatgttg ccgtgggcgt
     7141 ggtgggtgcg ctggtggcgc tgtttatctt cctcatgcgg acgccggcaa cacggctcgc
     7201 catcgaccgg atggtagtgc atctcccgaa gatcggaaac ctggtgcgca tcgtctatac
     7261 ggcaagcttt tcccggacgc tggcgtcgct gtataccagc ggtctgtcca ttctgaatgc
     7321 gctccagatc tcacgggaca cggtgaacaa cagctatctc cgctcccagt ttgacggctg
     7381 tatcaagagc atccgggtgg gaaatgccct gtcggaatcc attatggcga tggacggctt
     7441 tgacagcaag ctgggcgata ccatcaaggt gggcgaagag accggtaagc tggacgcaat
     7501 gctgctctcc accgcagacg actatgagta tgagtccagc gccgccatca agtccatgat
     7561 gtccattatc gaaccgctga tgatcgtcat tctcggtatc gttgtcgcag gcgttatggt
     7621 ctccgttctg gtgccgatgt actccatgta cggcaatatc gatgaaaata ccaccgcagt
     7681 tttcgcctgg atcgggcgga cgctgggcag atttttgggc taagagaaga gaaaaagagg
     7741 tgaactttcg tgaagaaaaa gggtgtttcc gctcacgtgt atatcacaaa caacaagata
     7801 caggtgctga ccggcagctg tgtgaagaat aagctccagg tcgtgaattt ttacgaagaa
     7861 cccctggaag agggctgtat cctcaacggc attatcatga acaactattc gctccagaat
     7921 acgctcaccc agatgtggca gaagaacaac ctgcccacaa aagatgtgac gctggtggtg
     7981 aacggcagtt ccattacggt gaaaccgctg aaaatccctc aggtgaatcc gaagaacgtc
     8041 ccgggtctta tccgggcgga gttcaaggat attgagggcg tacagaatat gctggtggat
     8101 tattccgtgg tgaaccccaa gaaccaggac ggctcgtgca gcatcctggc ggtgctttcc
     8161 acgaaggagt tcgtcaccag ctatatcagc ctgttcaaag aggcgaagat cgaactggat
     8221 gtgatcgacc tggtgcagaa ctgcctcatt aagatgatga aacggctcaa gtcgctccag
     8281 aataagacct atgccgtgtt tatcctggat aagaatatgc tgatgcagtg cctgttctcc
     8341 aacgacaact tcgtcatgac acgccgcagc cgcatcctgg ctgacccggc ggacgaaggg
     8401 ttcgaacggg aagtggggca gaacatcaac agcatcatcc agttcaacaa gtcggaacag
     8461 accggcgcag acatcaccga tattttcctc tgcggattcc ccggagattc ggtgcagatg
     8521 tttccgcatt ttgcaagcaa tttcggcgtg accgtccggg ctttcccgga ataccagccg
     8581 ggcgagctgg ggctgtcgga gggaatgaat cccggtgact ttatcgtgct gctgggcagc
     8641 ttgatccgct attcggacta aatcggacag gagggatttg gaatggcaaa aataccaaag
     8701 aaggatatga acctgtatcc tcttgtcgtt gcgccgaaaa agaataccgt gaaaaagggt
     8761 gctcttttcg ccgtggcagg tgcggctgca gtcgtgctgc tggtcggcgg ctatgtgggc
     8821 gcaagagtct atgtccacac ccaggaaaac ctggtggcag agatgcagga aaaggcgaac
     8881 gatgcggaac tcatggaaaa gatcaacaac gccaatgcgg tggcagcgga catcaatacg
     8941 ctccgcaccg ccgggaacgc ctacagtgag atacgggtga agattgacgg cagccaggaa
     9001 tactgcgata acttttctga cgacctggtg gaaaagctgg tctcctgcga agataccgta
     9061 agccccagca aaggtgcaac caaggtggcg aagatcacgg ggctgtccta tgacggttcg
     9121 gtgctttcca ttgatgcgta ttcgactgac agccaatatg tctccacgtt cgtggcgaat
     9181 ctgacacggc tgggactgtt ttccacggtc acctatgacg gctatacctt ttcggaaggc
     9241 acgtatacct atacaataag cgctgtgttc aagtcggcgg agacgacagt aactgacgca
     9301 gacggcaatc ccgtagagac gacaacggag gtggcacaat gacaactgaa gttactcgga
     9361 aacaggcgat attgctttct gtgctgtgtg ctatattggt cattatactg ttttggcaat
     9421 ttctgattag tccgcaaata aaaagtgcgt caaaagcaaa agaagatctg gaaacgctga
     9481 accagcagta ttccgatctg ctggtgcaga gcgagagcta tgaccagaac atggcggcgc
     9541 tggacggctg gaacgaaaag aacagcgagg agaccaaaaa actgtttccg ctgagcgatg
     9601 ccgagcgcat cgaccgcttt ctgacgtttg tcatgcacga atgcggcgtg accgtaaacg
     9661 ggctttccat cggacagacc atgcagtact acattgacgg cgagggcaat ctggtgacgg
     9721 cgtctccgga tgacgtggca aataccgccg gaacatccgg cacagacggt tctgccgcag
     9781 acggcagcac agcggcttat acggcaacgg gtgagtattg ctgtgacttt acatatacca
     9841 tggagggcga ttaccgcaac atgaaacaga tgctggattt tgtgaaccgg gtatctttcc
     9901 tgggaatcac cgcatactct ttcaacagtg tggaaaagca ggaaactgcc gcagcagacg
     9961 gcacgacgac cggaacagag gataaattcc aggatctgta cagctttacg atgaccatta
    10021 cagcgtatat gtataagagt ccgctggaga cagaccggga tacacagaca gaagaatcca
    10081 cagctgctgc cgcagaaacg ccggcagcgt aaaatgggtg atgccatatg aaacggaaaa
    10141 cagcacagcg aaatggctcg gcgattctct gggcggtgct ggtgatgctg atgatctcgc
    10201 tgatttctgc cgggatcatc ctcgttgacc ggatgtattt catgcgggag caggaggaaa
    10261 acaatcgtct ccaggcgaag ttctatgcgg aatctgccat cgaactggtg cagtcggata
    10321 tctgcaaaga gggaaccgca ctgacagacg gcacggacgg aacctatacc agtatgtatg
    10381 tcagcgccgg caataccgca gagaccttta ccatctcgtt cccggatgcc gcaaactgga
    10441 cttgtacggt gacggtcaac cactctgtgg tgaacgacag cgaaagcgat gtgaataaaa
    10501 agcggctgtc cggagagatc tacctcaccg caaaggtgac acgcaatacc agcggcggcg
    10561 gagaaaaaga gctttcagag gtttgtgcaa agctggcggt gaaaaacaac acctggcagc
    10621 tgatcggctt ttatcatctg taaggagatg ggcgtatgcg aaaacaacat aagggaacga
    10681 ctgtggtaga agtggtcatt gcctttgcga tggtctccat gtcctttgcc attggcatga
    10741 tcggcattgc gtccgggtcg aactttatca attccggcgc acggctgaaa aatcagcagc
    10801 ggaaaacggc tgcggcagac ctggacggga gcggtgcatc ggtgtcggac gataccatta
    10861 ccgtaaccgg aaaagacggc acgacaacga cgtttgaagt ccagaagcgt tcggcggacg
    10921 gatttgtgaa gtacagtccg aaatcgtaag cgctgtccgg cgcagaatgc cgggcgttcc
    10981 gctgcggcaa aggaggatga ccagatgcgg aggatagaaa aaaaccgaaa aggctttacg
    11041 ctggcagaag aagtcgtgac ggttctgctg atcggcattc tggtggcatc ggcgtcggca
    11101 attctgctga acgccatgcg tatcttctgt cagaatgtca ttatgttcaa cgcccagtcc
    11161 aagggcattg cggtcatgga acagctggag gatcggctga actatgcaaa agagatcggc
    11221 ggttcttacg gcggcatttc agatatttcc cgcaaggcgg actgtgcaca ccaggttttt
    11281 ctatcggtca aagatgacga tgacgggaaa aaagaactgg tggaggacgg ttatctgtat
    11341 tattccgatg cggaggacag caaaaatgag attcacaacg tcctctgcaa gctgggaaat
    11401 tatgatgcgg agatcgatct gaaaagcggc tccgggaaca cggcggttct gacggtgcgc
    11461 atcaagcgga atgacacggt gtactattcg gaggaacgta ccgtggcact gaaaaacagc
    11521 agcgttacgg taatcactgc tgcggattca tcccatgtct acagcaatac gggtacatcc
    11581 acatatttat acatcggcag tttggaataa aggtaaaaca acaggggaaa ggtgagagca
    11641 ttgaagaatt taccaatcgg cgaagtgctg aaggaatacg gctatatcac gcaggaacag
    11701 atcgaccagg cgctgcagtt ccagaaaacg cagaccggca aaaaggtgcg gtttggcaca
    11761 ctgctgaaag aaatgggctt tgtcacagag acccaggttc tggaggcgct ggggaaaaag
    11821 ctggatcttc agatggtgaa tctggaagag tacgaagtca atgtggatgt ggtggcgaag
    11881 gtcccgaaac agctggcaga gaagtataat gtcattgcgc tgtcggaaaa tgacgggaaa
    11941 ttgcagctgg caatgtccga tccgctgaac ttttatgccc aggaggatat ccgccagatc
    12001 gtggatatgc cgctggaggt gctgctgagt gaggaaaagc ggatcaagaa ggcaatcgaa
    12061 tattactacg ccgagatcag caccaagcag gcggcgaaaa aagcgaatat ttccgccgag
    12121 gatattgaag tgccgcagct caccgcagac gatgcggacg acgatgtgcc gatcatcaag
    12181 ctgataaact ctctgctggt gcgtggctac agtaccaacg ccagcgatat ccacattgag
    12241 ccgttcaagg atttcaccca ggtgcgtatg cgtatcgacg gacagatgat cagctatctg
    12301 acgctggaaa aggcgctgca taactccatc gttgcccgta tcaagattat gagcaatctg
    12361 aatattgccg aaaagcgtat cccccaggac ggcaacttcc acgctgtgct ggagggcatt
    12421 gatatcagta tgcgtatttc catcctgccc accacacacg gggaaaaggt agttatgcgt
    12481 tatctggttt cggataccaa gatcgaccac gccggacact tcggcatgaa cgattattcc
    12541 tacaatatgc tgatgaaaat gatgtctgcg ccgaacggca ttatctacat cacagggcct
    12601 accggttccg gtaagacgac cacgctttac atggtactgc agcgtctggc ggagcgtccg
    12661 gtaaacattt ccaccatcga ggacccggtg gaaaaggacc tgcccaatat cacccagaca
    12721 tcggtgaatg tgactgccgg catgaccttt gccgtgggac tgcgtgcttt gctccgtcag
    12781 gacccggata tcatcatgat cggtgagaca cgtgacgcag agacggcgga gatctccgtc
    12841 cgctcggcaa tcaccggaca cctggttgtc tccaccctgc acaccaacga tgcgatctct
    12901 tccatcgtcc gtctggcgga catgggcgtt gcgccgtatc tgatctccaa ctccgtggta
    12961 ggcatcgtgg cacagcgtct ggtgcgcaag gtctgccagt tctgcaaata cgaagagccg
    13021 gcaacggcag aggatatcgc tattgtgggc gacggcatcc ccatgattac ccggggcaag
    13081 ggctgtaagg tctgcaacaa taccggctat aagggccgta tctccatcca cgagatggta
    13141 tacatcgaca agacactgaa acgcatgatc gccagaagtg cagacagcca ggaaatgctg
    13201 gaatatgcaa aaaagaccca gggaatgcgc agtctgatgg acagcgccat cgacctggta
    13261 aaagagggcg taacaacgcc ggaagaggtc atgaagatcg cttattattc ggattaaaca
    13321 gggaaaggat ggtatactgc catgacaatg aacgaactca ttatgaaggc ccgggagaaa
    13381 aagtgttccg atatccatat cagcgaggga atgccggttt cattccgtat ccacggacag
    13441 ctgctggaca gcggcttttc gtttccggcg gcagaaacac gggaaatgat cctgtccatg
    13501 ctcagcgatg aacagcggga acagctggaa aacggcgacg acgtggactt tgccattgaa
    13561 acgccggact attgccgtca gcgtgtcaac gtgttccggc agatgaaaaa ggtggcggca
    13621 actatccgac tgctgaacaa caccatcccg acgctggaac agcttcagat gccgaaggtg
    13681 ctgcaaaatc tggcagacca gcccagaggt ctgatcctgg tgacaggacc gacgggaagc
    13741 ggtaagtcca ccaccctttc cgccatgatc gactacatca acaagaaccg ttcggaacac
    13801 atcattacca ttgaggaccc ggtggagtat gtgtatgagt ccggaatgtc cgtcatccac
    13861 cagcgtgaga tcggcgtgga caccaagagc tttgcggcgt ctctgcggtc ggcgctccgt
    13921 gaggatccgg atgtgatctt ggtaggagaa atgcgtgact atgagaccat ttccgccgct
    13981 gttaccgcag ctgagaccgg acacctggtc atgtccaccc tgcacaccac aggtgcggcg
    14041 cagaccctgg accgtatcat cgacagctgt ccgccacacg cccagaacca gatccggacg
    14101 cagctgtcca cgattctgaa gggcgttatc acccaggcgc tgattccgct ggcaatgggc
    14161 ggcggccgtg tggctgccac agaaattctg gtgggaacag atgctgtggg cagtctgatc
    14221 cgtgacaaca agtgcttcca gctggcaacg gtgatgcagt ccggcgcagc gctgggaatg
    14281 cacacactga acgctgacct ggcacgcctg gtgagagccg gcacgatccg ccgggaggat
    14341 gcgattcggg tatccaacaa caagaatgag attctggacc tgctgagtta aggaaaactg
    14401 cggcgtgtcc gtctgtagat tttcagagag gtgagagaga tgaaacaaaa tcgaaagggc
    14461 ttttctcttg tggagctgat cgtggtgctg gtgattatgg cgatcattgc ggcactgtgt
    14521 gcgcccaata tctctgccta tgtgcaggcg gcgaagatac agaactatca gactgtggcg
    14581 aacaatctgg cggacgaact ccagacccag ctgccccaga gccggtattg gaactgggac
    14641 gaggtaaagc agagtgccga ggggattctg cgctcagatg ccggaagagg cgtgacgctt
    14701 ttgagcagca gtgataccga ggaaaagtat caggtcaccg gtgccagcag cgatgccaat
    14761 gcggtgttta cggtgacgct gacctatcag ccggcagaca aaaccagcca gaaagtggat
    14821 gttgcggttt cctgcgatgg gtatgctgcg gcaaaggcgg agcagagctg tagtttggtt
    14881 ttgaagacga attatacgga tgcgggagcg tatccgatga gtaatactgt aactgaatca
    14941 aaggattctg gctggggtga tttaaaaaac agaataaaga cagataatga atggaaaaac
    15001 ttatttgcta atgacgaaaa ttataaaaaa tatgactttg gagttaatag tgttgtcgga
    15061 actcaagata gtgacagata cattattaat acaacagact atccaattaa tgcagtcaaa
    15121 tttgtacagt atggcatgca atttaaatat gacgggcaag cagtatatcc tataatgcag
    15181 gcgcaagctg gaacgacaaa cggtacattt ggctcaggtg gtcaatacaa cgtttgtggt
    15241 aataatggag attttaaaaa tgtatacatc tatgttggaa gtaaatctaa tgtggaaaaa
    15301 tata
//
LOCUS       DAWENO010000036        15288 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1611_length_15288_cov_11.592070, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000036 DAWENO010000000
VERSION     DAWENO010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15288
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1611_length_15288_cov_11.592070"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9372..9381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3676 a         3741 c         4516 g         3345 t
ORIGIN      
        1 gtccgctctg taaaaaaatg tgccgtttcc ggaaaacgct attgacgaac ctcccgattt
       61 attatataat aaaaagtaag atattgcggt atgcccggaa accgggaaca gtgccgcaga
      121 tgctgggaga ggagcgttac gcttttgaaa aaaatagaaa tttctgcctc gatcctgagt
      181 gcagacatgg caaatctgac cgctgtgaca gagggattgg aacgggatgg tctggatatg
      241 ctgcactttg atgtaatgga tgggcagttt gttccgaata ttacatttgg tatgccggtt
      301 ctggcggcag tagaccactg cacaaacctg tttctggatg tgcatttgat gatcgccgac
      361 cccattcgct atgtgaagca gtttgtggat gccggtgcag acctggtgac ctttcacatt
      421 gaaagcaaca gtgacccggc ggcgaccatc gctgcgatac acgatgccgg cgcaaaagca
      481 ggcatcgtgc tgaaaccggg aacgccctgg gaagaggcga taccctatct ggcacaggtg
      541 gaagttgtgc ttgtgatgac agtggaaccc ggattcggcg gtcagtcgtt tctctgggat
      601 atggtggaaa aggtcaaggc gctgcgtgcc tatgcagatg ccaaccagct gtcctatcag
      661 atcgaagtgg acggcggcat caatgcagag accgcacccc atgtgatcga ggcaggcgcc
      721 gagattctgg tcatcggcag ttatctgttc cgccaggctg acccgaaggc agccatggat
      781 gcgatccggg acgaggcagc aaaaatggca taaagccgca gaatgcagga aagaggaggc
      841 agaaacgtgc agtcgaaacg tcagccgaaa gtgcatacgt attcgaaaaa acggagacgg
      901 aaggtgcgtc ccggaagagc gatcctttcc atcggcttta cctttctggt ggctggtgtg
      961 atcggtgttg tgggctacag cgttgcaaag ccgatcctgc agtattccgg cgcagagcat
     1021 tctcagaccc agccggagga gacgctttct tctgcggcgg cagaaacggc ggcgccggaa
     1081 gagtcggcat ctgctgcggt gacagagacc acagcggcac cggtgcagac gcaggcagag
     1141 gacggctatg tgctgccggt ctcagcactg gaaagcaaac aggcgctcca ggctgcggcg
     1201 gcatcggcac gggagaaaat gccggacggc aaactcctgg tgattccgct gaaagtctcc
     1261 ggcggcgaga tctggtatca gaccagcgca tcgctggcga agtcctgcgg cgcagcaaag
     1321 ggcagtatga ccctgtcgga gatcgtggat accgtgaccc agcagggctg gacaccggcg
     1381 gcgcagctga gcctgctgtg ggataatctc ttgccggatg cggatgctgc ggcagggtat
     1441 cgtgtcacag acggcagccg ctggctggac aataccaagg aaaacggcgg caaagcgtgg
     1501 gcgagtccgt tcagcacagt gacggtgcag tatctgacgg agatcaccgg cgaggtgagc
     1561 cgtgccggat tctctcagat ctggtgcaag gatgctgtgt tcccggattt ccgggaatcc
     1621 gatctgaatt acatcggaga aagcgttcag aatccggagc ggaatgcggc gctggtacag
     1681 ctggtaaatc agctggcaca gactgccggc aatgtaccgc tctgggtaga agtggatgca
     1741 gcagatgcgg cagagggaac ggcagaagtg ttccagccgg atacactgca cgtttccggc
     1801 gttgtgctgg atcttgcgga aaatgcagcg caggcaaaga cgctgctgcc gcagataaaa
     1861 acaaaggcag gagatctgcc gctttgtctc acggcagatg ccggagcaga cttgcaggaa
     1921 ctgaaagcgg catcgcagga cggaaacggt gtgttcctgt cggcaacata agcgccggcg
     1981 ctgcatctca aaaaaggatt tgaaaagaac gactgctgcg gtgcaggcgt gcatatacat
     2041 agaaaaagga atatcgtgtt ttgaaacaga aacatgggaa aatgacagat aaggcggcac
     2101 tgcataaaac ttgtgcggcg ctgttctgac acagacaaag gagttggtca tagtggctgg
     2161 aaaatttact gtatcattgg gaaaaattat tgacgaattt catctggaaa ccatctatat
     2221 gccgaaagac ccggcagaaa ttctcattga cgaaaacgat gtgacccgtc ccggcttgca
     2281 gctgatgggc ttttatgaat actttaaccc ggaaagaatg cagatcatcg gcaagatgga
     2341 gtttgcctat ctttccacca tcgacgaaga aacacgtact cagcggctgg atgcgctgat
     2401 gtcccggcat atcccggcgc tcatcatcgc aagagaactg ccctatttct cggaaatgct
     2461 ggaccttgcg aagaaatacg aagtgccgct gctgcgcagt aaggaaagca cctcaaactt
     2521 catttccggt ctgatcgcat tcctgaacct gaaccttgca ccccgtatca cacgccacgg
     2581 cgtactcatc gagatctacg gcgaaggtgt tttcattacc ggtgagagcg gtgtcggcaa
     2641 gagcgaaaca gcgatcgagc tggtaaagcg tggtcaccgc ctggtggctg acgatgcggt
     2701 cgagatccgc aaggtctcca acatcagcct ggtgggcagc tctccggaca acatccgcca
     2761 cttcctggaa ctgcgtggca tcggtatcat caatgcacgc cgcctgttcg gtatcggtgc
     2821 ggtcaagaat acggagaaga tcgaattgat cgtggagatg gaacagtgga atccggaaaa
     2881 gatctacgac cgcatgggcg tggacacaca gttcgccact atcctgggcg taaagatccc
     2941 catgctgacc attccggtaa agcccggcag aaacctggca gtcatcctgg aagttgcagc
     3001 gatgaacaac cgtcagaaaa agatgggcta caatgcagcc gcagaactgc tggatcagct
     3061 gggactggac atggaggcaa aggaagtcgt aaaggattac gatgcgtttt aaggacctgt
     3121 gagctgacag cggataccgg cggacagtcg gataccgccg tttcgttacc acacgctttt
     3181 gcttgggaga caaaactatg aatgattggc aggaactcgc tgcggtttgc aggacgtgca
     3241 attgcagcgt acagtatgca gaacctctcc ggaatcatac cacgttccgg atcggcggtg
     3301 cgtgcggcgc ttttgtaaac ataaatacag aagagacgct gcgccggata ctcggtcttt
     3361 gccgtgaccg gaactatccc tacctggtgt tgggaaacgg cagcaacatc ctggcggcag
     3421 acagcggcta tgccggtgtt atcctgcatt tgggaaagga tttttcccgg attcagcagg
     3481 acggcgacac gctgtactgt gaggcagggg caacgctggg acgcatcagc agatatgctc
     3541 tggagcagtc cctgaccggt atggagtgcc ttgccggcat ccccggaaca gtaggcggcg
     3601 ccctctatat gaacgccggg gcatacggcg gcgaaatggc agatattgtg gtctcggcac
     3661 gctgtatgtc gccggacggc atgataacgg aagtgccgaa agcggaaatg gaactgggct
     3721 accggaagag cgttttccgg caggacggca ggatcattct gggcgtgacg ctgcggctgc
     3781 aaaagggcag ctatggagag atcaaggcag aaatggattc cctgctggga aagcgccgtg
     3841 ccaaacagcc gctggaactg cctagcgccg gcagtacgtt tcagcgtccg gtggggaact
     3901 atgcgtcgtt tctcattgac cagtgcggtc tgaagggcgc cagcgtggga gatgcgcagg
     3961 tgagcaagaa acacgccggg ttcgtggtga ataccggaaa ggcaacttgt cacgatgtgc
     4021 tggaattgtg tgattatgta aagcagacgg tattggaaaa gaccggctat cagctggaac
     4081 tggaaccggt ggtcatcgga gaccccggca agccggcaga gcagcccggc agctgaaaat
     4141 gccgtaacga gaaaggatgt ggagcgtatg caggaaacag aagatgtcag aatgcacgtt
     4201 gttgtagtga ccggaatgtc cggctccgga aaatcggtgg tcatggatgt attggaggat
     4261 atcggctatt actgtatcga taacctgccg ccgcagctga tgggaaaatt cgtggacatc
     4321 tgccgggagt cggaaaatca tctgcaaaag ctggcaattg ctgcggatct gcgctccggg
     4381 gatatgttta cagatgcgta ccggacactg ctggatatgg agcgccagcc ggatctggat
     4441 gtcaaaattc tctacatcga ggcggaagac gaggtcatca tcaagcgcta taaggagaca
     4501 cgccgcaagc atccgctgga tgagcgtttc ggcggctgcc tgcacaatgc gatcgcctac
     4561 gaacgggagc agctgctccg ggtaaagggc attgcggatt actatgtgga gacctcctat
     4621 ttcagtgcat ctcagctgaa agaacagatc cgggaaattt tcctggacaa ttcctcagac
     4681 tccatgtcca ttaaggtgac atcgttcggg ttcaagtacg gcgtttccac agagtctgac
     4741 ctggtgtttg acgtgcgctg tctgccgaac ccctactata tcccggaact gcgccaccac
     4801 accggttgtg aaaaatgcgt gcaggactat gtgatgtcct ttgaacagtc ccagacgctg
     4861 atgaaaaagc tgaaagatct cctggatttt cttatccccc tgtatattca ggagggaaag
     4921 agccgtctgg tgatcgcttt cggctgcacc ggcggaaagc accgttctat cacgttcaca
     4981 gagctgatcg gggactatct catttccaaa ggaatgcacg ttgttaagca gcatcgtgat
     5041 atcgggaaag accggtcgta acagcaagga ggagaacggt gtcattttcc aatgatgtac
     5101 gggaagagat cgccagaagc atcacggaca gagaccggca gttcgcctgt ctctatggca
     5161 tcctgctgta ttcccgtatc tttaccccgc agcaggtagt gctgcaaagc gaaagcccgg
     5221 tgtttgcccg gctgatgccg gttttgttcc gtgcagtgtt ccaggcggag ctgcgtccgg
     5281 acgtgcggac agtttcccgt ggcggagacc agtttatggt cagctatacc attacggatg
     5341 cggcgcagat cgggcggatc tgtgaggtgt accacatcca tcccgaaaag cgggagattc
     5401 gtctgtccaa cctggtgaac aacagcctca gcgcatttct cgccggggtt ttcttcggct
     5461 gcggcagcgt cattgacccg aacaaggaat accatctgga attcaacacg ccctctgcgc
     5521 tgctctgcgg agacctgcaa aaggtgcttg gcagcatcgg cgtggcaggc agatcgctgg
     5581 tgcggaagaa tatgtatgtg ctgtatctga aagacagcga gcatatcgag gacacgctca
     5641 cctgcatggg cgcccagcag tgtaccatcg agctgatgaa cattaagatt cgcaaggata
     5701 tgcgcaataa ggcgaatcgg gtgcggaact gcgacgaggc gaacatcaac aaggtggtca
     5761 gtgccgccat gcgtcagatg caggatatcc agctgattat ccgatgcggt cagctggaag
     5821 cgatgtcgcc ggaactacag gaactggcag aactgcgcct ggaaaatccg gaactgagcc
     5881 taaaagaact gggcgaactg ctttccaatc ccatcggacg ctccggtgtc aaccaccgct
     5941 ttcagaaaat cgccgctatg gcagaggagt tgagaaaacg tgccgcaaat tcctgagaat
     6001 gcctttgtgc atctccatgt ccacagcgag tacagcctgc tggacggtgc ctgccgcatt
     6061 ccaaagctga tcgagcaggt gaaagccatg ggacagacgg cggtggcgct gacggatcac
     6121 ggcgtgatgt acggcgcata tccgttttat gaggcagccc ggaacgccgg aatccgtccg
     6181 atcctcggct gtgaagtcta tgtggcaaaa cgcacccggc acgaccggga gcatctgctg
     6241 gatcggaaaa gctatcatct gatcctgctc tgtgaaaatc aaaaaggcta tcagaatctg
     6301 gtgaagctgg tttcgctcgc cagtctggat ggcttttatc aaaagccccg tgtggactgg
     6361 gaattgctgt cccagtacca tgagggctta atttgtctgt ccggctgtct cgccggagag
     6421 gtggcgcaga aactgacagg ggatgactat gccggcgcca aggaaacggc gctgcggtat
     6481 cgtaccttgt tcggcgcaga aaactatttc ctggagattc agaatcatca gatccgggac
     6541 gaactgcgca atctgcccca gctgatacgg ctttcccgtg agacggggat tccgctggct
     6601 gccaccaacg atgcccacta tatccgccgg gaagatgctg ccatgcagga ggtactgctc
     6661 tgcatccaga cgggaaaaag cctggatgac ccggagcata tgcacttcga gaccaacgag
     6721 ttctatctga aatccacggc ggaaatggct gcgctgtttg cagatgttcc ggaggcagtt
     6781 accaacactg cgaagattgc cgagcgctgt catgtggagt tccagaccgg aaagatctgg
     6841 ctgcccaagt tcaccatgga cggcgtgtcg gactgccgaa agctgttttt gcagctgtgc
     6901 cgggacggct tgtatcagca ctacggtgcg catccgtcca aggaggtgct tgaccggctg
     6961 cgcatggaaa tttccgtcat taccgaaatg ggttatgtgg actatttcct catcgtgtgg
     7021 gattatatca acttcgcacg ccgcaatgat atcccggtgg gaccggggcg tggctccggt
     7081 gcaggcagtc tctgtgctta ctgcatgggc attacccaga tcgaccccat ccggtatcag
     7141 ctgctgttcg agcgctttct caatccggaa cgtgtcagta tgcccgactt cgatatcgac
     7201 ttctgcatcg agggcagaca gcgtgtcaag gagtatgtga tctcccggta cggcgcagac
     7261 cgtgtgtcgg agatcatcac attcgacttg atgaaagccc gtggcgcagt gcgggatgcc
     7321 ggacgggcga tggggatttc ctacggactc tgcgataaaa ttgccaagtc catcgactcc
     7381 agaaatacca ttgcccagac catggagagc aaggacgggg cagatctgcg gaaactgtat
     7441 gagacggatg ccactgcgaa aaagctgctg gatatggcga tgcagctgga gggaatgcca
     7501 cgccatgcct ccacccatgc cgccggtgtc ctcatctcgg cagttcccat tacagacctt
     7561 gtgccgctcc agcgcaacga tgagaccatt gtgacacagt atcccatggg cgtgctggag
     7621 catatgggac tgctgaaatt cgactttctg ggactgcgga atctgaccat tatccgggac
     7681 tgtgtccggg cagtgcagca gtacgaaccg gatttctcca tggatgctat atctctggat
     7741 gacccggcag tgtacgagat gctgtcccgt ggggatactg ccggcgtgtt ccagtttgaa
     7801 agcgccggga tgcgccacgt gctgatgcgt ctgaaaccac gctgcctgga agatctgacg
     7861 gcggtgcttt cgctgtatcg tcctggtcca cgctcgtcta tcgacctcta tctggaaaac
     7921 cgcagccacc cggaaaagat cgcctatgcc catccgattt tgaaaccgat tctaaaaccg
     7981 acctacggct gcatggtgta ccaggagcag gtcatggaga tctgccgggt actgtcgggc
     8041 tattcctacg gcagagcaga cattgtgcgc cgtgccatgg caaagaaaaa gcaggatgtc
     8101 atggaacggg agcgccaggc gttcgtctac ggcagtgacg gctcagacgg cagttcgccc
     8161 tgctgcggcg cagtggcaaa cggtgtctct gcggaaacgg caaacgccat ttttgatgag
     8221 atcgccgtgt ttgcagacta tgcttttaac aaatcccatg cggtttccta tgcgtacctg
     8281 gcgtatcaga cggcatatct caaggtgcat tattttgcgg acgatatggc ggcactgctg
     8341 accagcgtca tgggcgatac caccaagact atggaatacc tcaccgcctg tcagaatgcc
     8401 ggggtaaaag tcctgccgcc tcatgtcaac gaaagcacgg cagtatttgc aaagtgtggc
     8461 gaccacatcc ggttcggctt gctggcagta aagaacctgg ggcgtggctt tatccaggca
     8521 atgacggaac agcggcagaa agagggaaac ttcactagtc tggcagactt ctgccggaga
     8581 atgctgccct gcggtctgaa taagcaggca ctggacagcc tgattcgctg cggtgcgctg
     8641 gacgggctgg gactgaaccg gcggcagatg atcgagttcc aggaacaggt gctggcagac
     8701 tgccgggacg agcggaacag cattgtgacc gggcagatga acctgttcgg cggtgcagac
     8761 gaaagggcag tccaggagac gcagattccg ccgctggcag agtatgaccg tgcatttctg
     8821 ctccagctgg aacacgacag cctggggctt tatctttccg ggcatccgct ggaacaggtg
     8881 gagtggctgc gccggctttg tcacgccgtg gatgcggcat cgctccggga acagaaagat
     8941 ggtgcatcgg tactgctgat cgtgacgctc cagcacatca aacagcacac cacaaaacag
     9001 ggagagacca tgtgctttct cacctgtgag gacaggagcg gcacggcgga ctgcgtggtg
     9061 ttcccgtccc tgtatccggc ggtgcggcag tatctggaaa aggaggctgt cctgtgcatc
     9121 cgtggaaagc tgacgcagaa ggaagagacc gtcagcgttt tgtgcgacag cgttctgacg
     9181 gaggaaggat tccggcgcac catgtcccag ggcaggctct gcatcaagac ggactcccgg
     9241 cagacggaaa agctccacgc acttgcggtg ctggtgcagc agtatccggg agatgtgccg
     9301 gtctgctttt atctgaccga ccggaaaaag atgctctctc tgcgtggcgg acaggcgatg
     9361 gcggtgcagc cnnnnnnnnn ncgtatgccg cactctgcag actggttccg ccggaacaga
     9421 tcgggttcct gccggcaaaa tagacatttt cattttttgt gcagagttgc tcaaattttg
     9481 catggaactt gttaactttt tcgaatacca cttgacaacc cgggaaatat cagtataata
     9541 gaataggaat ggaatggtga atgcgtatgc ggctttcaag ctgcgctgcc gtttcaaaat
     9601 tttgccatgc ggcggaatgg agaatacgtt tatgattaaa acgattggtg tattgacaag
     9661 cggcggcgat gcgccgggta tgaatgcagc agtacgtgct gttgttcgtg cggctctgaa
     9721 aaagggcatg acagtatatg gcgtaaagcg tggctactgc ggcctgctgg acgatgacat
     9781 tgagccgatg gatgagatga gtgttgccgg tattatcaac cgtggcggca ccatgctgta
     9841 cactgcaaga agtgcggagt tccgcaccga agagggtgtg ctgaaggcaa aggctacctg
     9901 tgaaaagtat ggtctggaag gtctggttgt catcggcggt gacggttcgt tccgtggcgc
     9961 agcagatctg tctgcacacg gcatcctgtg tgtaggtctg ccgggtacca tcgacaacga
    10021 tatcgcctgc actgactaca ccattggtta tgatactgca atgaataccg caatggaact
    10081 gatggacaag ctgtccgaca ccagccagtc tcactatcgc tgcagcgttg ttgaggttat
    10141 gggtcgtggt gcaggttaca ttgcgctgaa taccggtgtt gcatccggcg cactggagat
    10201 catcacaaag gaaaagccgt atgatctgga taagatcgct aaaaagatga tggaattcaa
    10261 ggcaagaggc aagcagaact tcattatcgt tgttgcagag aatatcggtc attcggaaga
    10321 gatctgcaag ctgatccagg aaaagaccgg cattgaatcc cgtgcaacca ttctgggtca
    10381 cgtacagcgt ggcggttctc cgaccgttcg tgaccgtgtt gctgcaactg aaatgggcta
    10441 ctatgcagtt gaactgctgg agcagggcat cggcaaccgt gttgttggta tgcagggcgg
    10501 taaggtcgtt gacttcgaca tccaggaagc tctgtctatg acaaagccgt atgatgaaaa
    10561 gctgcatcag atcgcagaag atgttgcatt ctaatgaaac tgtaaaagaa tgctgaaaag
    10621 gggtggacgt gcgaaagtgc gtccactttt ttgtttggca tatctggaaa acatagaatc
    10681 ccatgcgcag tcagattgcg gattccccgg catatccaat cggatgcctg tggatttttt
    10741 aggcaatctg ctgaaaaaac agctgcaaat tttgtgcgca ggctttattt tgtgtttccc
    10801 ttgcaatttt gtgcaattta cagtataata gaaatgatat gttgtccgga agaatttccc
    10861 ggtagggata tttccggcag aaaaagaaag aaggagaatg tacatggata ccattggatt
    10921 tattggtgtt ggcaatatgg gcagtgcgat tctgaaaggg atcgccggca gcagcctgaa
    10981 acaggaaact tcactgttcg catacaaccc gaccgaggca aaagttgatg ctctggcaaa
    11041 ctgcggcgtg cagaagtgcg ggagcgcagc cgaagtggtg gaaaagagcc ggtacgtgtt
    11101 cctggcgatc aagccgcaga agtttgaaga ggtactgccg cagatcgcag atgcagtgac
    11161 agaagagacg gttctggtct ctatcgctgc cggcatcggt gcggacttta tccggaaaat
    11221 gacaaagccc tatgcaaaag tggttctggc gatgccgaac acaccgctgc tgctgggcga
    11281 gggcgcaact gcactggcga aagatgatgc cgtcagcgat gcggaatttg ctgtggtaca
    11341 gcgcatcttc ggcagctgcg gcatgaccgc tgtcattgcg ccagagcaga tgaaagaggt
    11401 catcgccatt aacggcagca gcccggcttt catctatctg tttgcgaaaa gctttctgga
    11461 ttatgccgca aaagtgggtc tcccggagac atcggcaaag gaactgtttg cgcagtcgct
    11521 gatcggttct gcaaagatga ttacggactc cggctattcc atcgatgaac tgatccgcca
    11581 ggtctcttcg ccgggcggaa cgacgcttgc cggtctggat cagctgtacg agggcaagct
    11641 gtccgatgct gtccagaatg cctgcgagag ctgcaccaag cgtgcgtatg aactagcaaa
    11701 gtaagaacct gttttcacaa gacacatata tacacttgtg aagacaggtc ttgagaaatc
    11761 ataatcaaga aatataccgc ataggatgag agcaggtaca agccaatttg caaagcaaag
    11821 gagcgatctg tgatgatccg aaagcgaaca acactgacaa caacggaacg cagacagcgg
    11881 ctgttctgcc tgctgtttgg ctgcatttta ctggggacag cctggggcat ggtcatgctg
    11941 cggtggcagt atgacggcgt gcctacgggc tgggcaggct ttctgggaat gttttttcag
    12001 accaagccta ctcttccggc actctggacg gagagcgcag gggacatcct gctgttcctg
    12061 ctggcgtttt ttctgctggg cttttcggct gtgggacagc cttttgcgtg gctgctgctg
    12121 ctgacctacg gcgtgtgcat gggcggcacc ttgcagacac agtgcagtgc cggcggcgct
    12181 tttgtgcgca gtacgacgct gctgtgctat tttgtgccga tgtcggcgct gctggtgctg
    12241 gcggcgaggg aatccctgcg gttctccggc tatttcactg cctacggttt ccggaatgag
    12301 ccggaagagc agatgtatca ccagctccgg atgtactgcg ccagattcgt tgtgcttgca
    12361 gtgtttctgc tgctgttcgg gttcgcctat gggacggcgt tctatgcggc ggtaaaataa
    12421 gggcgtgctt tctttccggc acgctcttcg gaaaggaagt ttgtctatgg gtctgtttgg
    12481 atcgaattac gagcgtgccg gttccggtat cgcaaaaaat gcaccgaaga aaaagggctt
    12541 tttccgcttt tttgagatat tcggcagaaa gttctggaaa ctgtttgagc tgaatctgct
    12601 gtattttctc agctttttgc cggtgatcgg cgcagttgtc ctggcgcttg ccatcagttc
    12661 ccagctgggg ataattctgg cggcagcctg tctggtgctg tttgctgcaa tcatcggacc
    12721 ggcaacggcg gcgcatacga agatcctgaa aaattttgtc atggaaaagc ccacgttttt
    12781 ggtgcatgac tttttcaagg cgtttcgttc ggaattcaag tattcctgca tcgtcggcgt
    12841 gctggactgc ctgctggcgt gctgcatctc ggcggcgttt tatgtgtatc cgaaacttat
    12901 tgagcagtcg gggagcaagg tgtactacat ctttttcgcc attacgctga gcatcggcgt
    12961 catcgcactg atcatgaatt tctacatttt ccccatgctg gtttccacga acctgtctct
    13021 gaaaaatgtg ctgaaaaatt ccctggcgct gtctgtagtc gccttgaaaa ccaatatcat
    13081 caccacgctg atcctggcgg ctgtgatcgc agtttatgtg ctggtattca tgtttgtgaa
    13141 catcaagtac tccatgatcc tgctgttcct gctgccgttt atgccggcat cctggttggg
    13201 actggtgatt acgttccagt cttatcccat cattcagaag tacatcatca acccgtacta
    13261 tgaacagcgt ggcgaggtca atccggaact ggcgcaggtc tctgcgtcgg aagaggagac
    13321 cgtgttcgag gacatgggcg gcaaggaaaa gcccattgaa ccgccgaaaa aagctggcgg
    13381 aaagcagtcc ggcggaggac gtactcacaa gggaaaagga aaaattattt cttaatttac
    13441 aaaataaata ctttacaagt gccggaaaat gtggtataat aacaaaggtc atgtacttgg
    13501 aatctggagt aagccggaga aaagcggatt tccgctttcg taacggaata tcacctgcct
    13561 ttttctatgt gcatgataat actaaatgag gtgaaagaaa atgctgctga aagaagaaaa
    13621 aactgagatc atcgagaaaa accgtaccca cgagaacgat actggttctc cggaagtaca
    13681 gatcgccatt ctgacaaaga gaattaacga tctgactgca catctgaagt ctcacaagaa
    13741 cgaccatcac tccagacgtg gtctgctgaa gatggttggt cacagaagaa accttctgaa
    13801 ctatctgaag aagaaggatg taaaccgcta tcgtgcaaca atcgagaagc tgggtatccg
    13861 taagtaagat acaacgacga aaaaagggaa aggcagtaca gagccgagtg ctttgtctgc
    13921 ctttctgtgt atgataacga ttccaaaaac aggcattcgc attaagccgt gtacttcatc
    13981 gggcgctgca attgtcgaag tatagggctt tttgcgaatt cgggtggctg gaatcaggaa
    14041 aggaaagaca agatatgttt gagaacgcac aacgatttga aacaaccttt gccggcagac
    14101 ccctttcggt cgagaccgga aagacctgcg ctttgtcgaa cggttcctgc tgggtacgct
    14161 acggcgatac cgtggtgatg gcgaatgtca ctgcaagcgc aaagccgaga gagggcatcg
    14221 actttttccc actgtctgtg gactatgagg aacggatgta ttcggtggga aagattcccg
    14281 gctctttcac caagcgggaa ggaaagcctt cggaaaaggc gattctgacc tcccgtatgg
    14341 tagaccgtcc gattcgtccg ctgttcccga agtccatgcg gaatgacgtt tccgttgtca
    14401 tgacggttct ggcagtggac caggactgcg caccggatat tgtgggcatg atcgccactt
    14461 ctattgcgct gtccatttcc gatattccct ggaacggacc gattgccggc atcagcgtag
    14521 gactggttga cggcgagatc gtgctgaacc cgacgctgga acagcgccag cacaatgacc
    14581 tgcatctgac cgttgccggc actaaggaca agatcgtcat gatcgaggct ggcgccaatg
    14641 aagtgccgga ggataccatg ctccaggcaa ttctcaccgg tcacgaagag atcaaaaaga
    14701 tggttgcctt tatttccgac atccaggcaa agatcggcaa ggagaagttt gtctttgagg
    14761 cgcacgaggt agaccaggaa ctgttcgaga atgttgatgc aatggtgcat gaccaggtgg
    14821 cacacgctct ggacaccaac gacaagaacg aacgggatgc acgcctgcgc ccgatcattg
    14881 atgcggcaca cgagactttc gatgccgttt gcggcgaaga gaccggcatg attgacgaga
    14941 tcctgtacct catccagaag aagatcgtcc gtgcatggct ctatgagggc aagcgtgtgg
    15001 acggcagagg cattgatgag attcgtccgc tggcggctga ggttggcgtt ctgccccgtg
    15061 tacacggcac tggtctgttt acccgtggtc agacacaggt gctgactgtg gcaacactgg
    15121 gaccggtcag cgacagccag aaactggacg gtctggacga agagacctcc aagcggtata
    15181 tgcaccagta caatttcccg tcctactctg taggcgagac aaagcccagc cgtggaccgg
    15241 gacgccgtga gatcggtcac ggcgcactgg cagagcgtgc gctgattc
//
LOCUS       DAWENO010000037        14724 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1687_length_14724_cov_11.636990, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000037 DAWENO010000000
VERSION     DAWENO010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14724
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_14724_cov_11.636990"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13100..13109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3522 a         4074 c         3713 g         3405 t
ORIGIN      
        1 ggtgcatata ttgcagctgt gcctcttttg catccatatg gtttcctcct ttttgtccga
       61 tttcggactc gcaatgaata ctactatacc acaaaatttc ggcaatgtca agcaatttct
      121 cagccgaaaa acaaaaaacc ggtgcaggct ttccacccac accggttttt gatatattct
      181 gctgttttac agtcttgtct gttcgtctgc cgtgatttgc tgcaggactt cgctcgccag
      241 gtacagcgag ccgcagatca ccaccatacc gccgtcggct ttcgcctgcg ccagcgcatc
      301 ggcgtatgcc gcctggaatg atgccgctgt ctgtacgccg gcatggtctg caaagcacgc
      361 cgccagttct tcccgtggca cagcgttctg cacgaagtca tccacacaag tgaccgtatc
      421 cagcacaccg cccagtatct gacaagcagt gtgatagtcc ttggtggaga tcatgccgca
      481 gatgccgtga atgggtttct gtccagcaga gcgcagcaga tctgccagcg ccataacgcc
      541 tgcctggttg tgaccgccgt ccagcagcac catgggatct ttccgcagca gttccgttcg
      601 tgccggaaca cgcaccttgg aaaaggcatc gttcgccact gccgccgtaa tcagatatcc
      661 gtgcattccc aggaatcgaa tcacctcgat agcagtcatg gcgttgtaca cctggtgacg
      721 ccccggcata tggagcgtat aggtgaaact gtggtactgg aacaccgtct cctccagcgt
      781 ttccgaaaga atccggcagt cctgcatatt ggggatcacc agctgtgcat ttttctgctt
      841 tgccgtctcc tgcaccaggc agaccacatc catgggatta tccgtggaca acaccaccgg
      901 acagccctgc ttgatgatac ctgccttttg tgcggcgatc tctgtgagcg tgttccccag
      961 cagagccata tggtcgaagg atacggaagt aattgcgcag accagcggct ttttcaccac
     1021 attcgtggca tccagcaagc cgccgatgcc ggtttccaga accaccagat cacagtgctg
     1081 ttccagaaac cagagcagcg caatggcgaa agtgatctca aactgtgagc agtccgccga
     1141 aacgggacac gccttcacct gttcgcagaa ccggcagagc gcctcgccgg gaatatcctg
     1201 cccgtcgatc tggatgcggt cggtgtaatg ctggataaac ggcgacgtca gcgtaccggt
     1261 tcgaaacccg gctgcggtgg ctgccgccgc acagaaagcc gtcacagagc ctttgccgtt
     1321 tgtcccggca atgtgaatca cctgtaactg ctcctgggga tttccaatcg catccagcag
     1381 cgcctgtatc cgggacaagt catgcaccgg cttgccgctt ttctgatatc cttgaatata
     1441 tgccattgct tcggaaatcg tcattgcatt cactcccctg tccgacgttc agaagttctt
     1501 ccctgttctg taattactgc aaagccgcca tagactgcat gatcttgtcc atcttctcct
     1561 gtgcctttgc cagctttgcc ttttctgcct ctaccagctt ttccggagcc tttgccagga
     1621 atccctggtt cgacagtttc ttgctcagga agtccagatc cttctgcacc ttttcctgtt
     1681 ccttcttcag ccgtgccagt tccttgtcct tgtcgatcag ctcgtccata gggataaaga
     1741 tacggacgct gtcggtcact gcggtagccg catccggcat ctcgaacttt tcgcccactt
     1801 ccacagcaga tgcagacgcc agcttttcaa agaatacggc agtgctgcgg tacagctccg
     1861 gctctgtggt ttcgatgcag acacgtgcct ttttggacgg cggaacctgc atttcggtgc
     1921 ggcgattccg gatggcacgg atggcgtcaa tgaccttttc gaactgtgcc gcatcggcat
     1981 agtcatactt tacatcataa accggatagg attccacgat aatcatggac tcgccgccgg
     2041 tcagcacctg ccagatctct tcggtgatat acggcataaa cggatgcagc agtttcagca
     2101 taccacgcat tacccaaacc agaacagcct gtgccttttc catggttgcg ccgcctgcct
     2161 ggatacgggg ctttgtcagt tcaatgtacc agtcgcagta aacatcccag ataaagtcgt
     2221 agatctttgc gcttgctacg ccgatctcga acttatccag attctcgccc acttccttgg
     2281 caactgcatt gaacttggaa acgatccagc ggtcttccag ttccagatct tccggcagtc
     2341 cgttgtactg gaaatcttcc ggcagattca tcataataaa acgggacgca ttccacagct
     2401 tattggcaaa gttccgggca gcctttacct tgtcatcgct gtagcgcata tcgttgcccg
     2461 gtgcattacc ggtcgccagc ataaagcgca gagcgtcagc gccgtattcg tcaatgactt
     2521 tcagcgggtc aataccgttg cccagagact tggacatctt tctgccctgc tcgtcacgta
     2581 ccagaccgtg gatcaggacc gtatcaaacg gcacttcgcc catgttcttc aaaccggaga
     2641 acatcatgcg gactacccag aagaagatga tgtcatagcc ggtgaccagt gtattcgtcg
     2701 gatagaaata tttcagttcc tctgtattat ccggccagcc cagagtcgag aacggccaca
     2761 gcgcagagct gaaccaggtg tccagagtat ctgggtcctg gcgcatgggc ttgccgcact
     2821 tcgggcagac tgcgtggtct tccttggtaa cgaccatttc gccgcaggca tcgcagtaga
     2881 atgccgggat ctggtgaccc caccacagct gacgggaaat gcaccagtcc ttgatgtttt
     2941 ccagccagtg gaaatagatc ttgttgaaac gctccggcac aaatttagtc ttgccgctct
     3001 tgacagcatc aattgccggc tgtgccaggg gtttcatctt gacgaaccac tgcttggaaa
     3061 ctctcggctc aacggtggtg ccgcagcggt agcaggtgcc gacattgtgg ttatagtcct
     3121 cgatctccac cagagcgccc tctgccttca gatcttctac aattgccttt ctcgcctcat
     3181 agcgatccat gccggcatac ttgggatatg cgtctacgat cttggcatcg tccgtcatca
     3241 cgttgataac ttccaggttg tgccgcagac caacttcaaa gtcgttcggg tcgtgcgccg
     3301 gtgtgatctt taccacgccg gttccgaagt ccatctccac atagtcatct gctacaatgg
     3361 gaatttcacg gtgtaccagc ggcaggatca gagtcttgcc caccagatcc ttgtaccgtt
     3421 catcattcgg gtttactgct acggcagtat cgcccagcag agtttccgga cgggtggtcg
     3481 ccagttccag atagccgctg ccatccttga gcggatagcg caggtgccag aagtgaccag
     3541 cctggtcctc atattctacc tctgcatcgg aaatggaagt gagacacttc gggcaccagt
     3601 tgatgatgcg ctcgccacgg tagatcagac cttcctggta atacttgatg aaaacttcct
     3661 tgactgcctt gctgcagcct tcgtccatgg taaaacgctc tctggtccag tcacaggaag
     3721 aacccagctt tttcagctgt tctacgattc tgccgccgta ctgctttttc catgcccagg
     3781 cacgttccag aaatgtctct ctgccgatgt cttccttttt aacgccctct ttgcgcattg
     3841 cctccacgat ctttgcctct gttgcaatgg atgcgtggtc ggttcccggg agccacaggg
     3901 tgctgtagcc ggacatacgc ttccagcgga taagaatatc ctggagtgtt tcatccagcg
     3961 catgacccat gtgcagctgt ccggtgatgt tcggcggcgg aattacgatc gtatacggtt
     4021 ctttctcaga atccacgtgc ggggtaaagc tgccgctgtc attccacatc tgataaatgc
     4081 ggtcttcaaa gtctttcggc tggtaagatt ttgccagttc ctgtttcata aatgttcgtt
     4141 cctttctatt cgaatcgccg actgccaata aaaaaagccc cggcaatcct ggaaattaga
     4201 ttgctcagga cgaatcagct tgggctgtcc gtgttaccac ctgaattcgg caaaacgccg
     4261 cactcattgc tgcgctgtca cgggcgcacc cggtgcagac tacgcacctt ccgttttccg
     4321 gaaagcttcc cctgcactgc tctgaggcta cctttttctg cgccgcccga aacttgcacc
     4381 aaccgttttc tctctgtcag agtcagccgc agaatactct tcctcttccc tgcatttcac
     4441 acatttctta ttatatcggt tttccgccgg tttgtcaagg gcgattttgg gaaaatttat
     4501 agttgacaat tccttgaaaa tatactataa taaaataatg tctgagagtc tgccggaaca
     4561 cagtacagga tctgcggcag cggcaggact ctaggcagca ctgtgcaaag cccacaggcg
     4621 gatttattgc agtgctgccc tatcacgaga aaccaaacca cggcggcact tggcgttctg
     4681 cacctgctgc ctggaatcaa aggagcatga gattatggca aagaaacaga tttcaaaatc
     4741 cggtgcagca aagctgcggg aagaactgga ttttctggta accgtccgcc gtgcagaaat
     4801 ggcacagaaa ctgaaagagg cacgtgcgca gggcgacctt tccgagaacg ccgaatacga
     4861 tgaggcaaag aacgaacagg caatcatgga agcacagatc atggagatcc agtacaccct
     4921 ggacaactct gagatcgtag acgatgacag catttccatc gacgaagtcg gtctgggttc
     4981 taccatcaag gtaaagaact tccgcaccgg caagatcgag actctccaga tcgtcagcag
     5041 caatgaggca aacttcaaag agggcaagat ctctgacgag tcccccatcg gcaagggcgc
     5101 tctcaaaaag aaagtcggcg acagctttat gatcgaggca cctgccggcg aactgaagtt
     5161 cgaaattctg gaaatcagca agtaattcaa aatagatata ggagtgaatc caatggcaaa
     5221 tcagaacaca ccgtccgttc cggagcagga acaggacagc aatgccctgc gccagattcg
     5281 tctggataag ctgcggcaga gacaggcaga cggcaacgac ccgtttacca tcaccaagta
     5341 tgatgtaact gcaaaggcag ccagcatcaa ggcggactat gaagtcaccg aggcaaagtt
     5401 caaggaagag gccggcgagg acgaagaggc gctccaggca aagctggata cgctgaagga
     5461 acagccgatc tccattgccg gccgtattat gagctggcgt gacatgggac gtgcaaactt
     5521 cattgatgta cgggattcca ccgaccgcat ccaggtttac gtccgcatga atgacatcgg
     5581 caaggaaaac tttaaggaat tcaagaagtg ggacatcggc gatatcgtcg gcgtcaaggg
     5641 ctttgttttc cgcacccgca agggcgagat ctctattcat gcaacagaga tcaccatgct
     5701 ttccaagtcc ctgctgcccc tgccggaaaa gtggcacggt ctgaaagacc aggatacccg
     5761 ttatcgtcag cgctatctgg atctgatcgt aaacccgaac gtcaagcaga ctttcgttac
     5821 cagaagtcag attgtccggg agatccgtgc gtatctggac ggcatcggct atctggaagt
     5881 agagacaccg gttctgctgc ccctgcagat cgctgcggct gcaagaccgt ttgtcacaca
     5941 tcacaacacg ctggacatgg atatgttcct gcgcatcgaa accgaactga atctgaaaaa
     6001 gctgatcgtc ggcggtttcg accgtgtgta cgaagtggga cgcattttcc ggaacgaggg
     6061 catggatccg tcgcacaacc cggaattcac caccatcgag atgtaccagg catacaccga
     6121 ctatcacggc atgatggatc tgatcgagga tatgtaccgc acactggcaa agaagatctg
     6181 cggcaaggat attatttcct accagggcac agacattcgt ctgggcgaac cgtgggagcg
     6241 tctgaccatg gcagaggccg tcaagaagta tgccggcgta gattacaacg actgggcaac
     6301 ggatgctgac gcaagagcct gcgccaagga aaagggcgtg gaagtcgaag aaggcgaaaa
     6361 tgcaaccaag ggtcacgtac tgatcgcatt ctttgatgcc tttgttgagg aacagctgat
     6421 ccagccgact atcatttacg attatccggt agagaattct ccgctggcaa agcgcaagcc
     6481 ggaaaatccg gcgttcacag agcgtttcga gtacttcatc tatgcccgtg aaatgggtaa
     6541 tgcgttctct gagctgaacg atccggtaga ccagaaggaa cgttttgtca agcaggtaga
     6601 ggctcgccgt gcattgggcg acatgaccgg tgaagtagac gaagatttcg tcaccgcact
     6661 ggaatacggt atgccgccga cgggcggtct tggactgggt ctggacagac tggtgatgct
     6721 cctgaccgac agcccgtcta ttcgtgacgt actgctcttc cccaccatgc ggccgctgcc
     6781 gaagctggga cagggacagg atgaagagga cgctgcggaa gaagaaaccg aggcgtaagc
     6841 aaaagcaata cattgaaatg ataaggggga attggtacag atcatttgta tctgtgtgca
     6901 ttcccccttt ttatattctc taaaaaagtg aggtcacttg atgcaggaag aagaaaaaca
     6961 aaacggctct tccggcacgc cgtcggagca gccgaaaaag cacaaaaaag agaagggcaa
     7021 gtctctgatc gaaaccatgc gggaaattga cgccaaagag gcgcagaaag aggctgaggc
     7081 agaagaacgc cggcaggaaa ttctcgcaga gcgtgagaaa aaagaaaaag aggcatacgc
     7141 caaaaaaatc cagcaggacc gcatcgaatt gatgcggcta aaacagggca tcattaccga
     7201 gagcgacacc atttatgagg aaaaggaaga gaaacctaag ctttcctttt ggaaaaagat
     7261 ctccaacttt ttataccaca acaagtggtg gctggggatc actgtcttta ttgcaggcat
     7321 tttcgtgttc ctcattgtgg attttgtcac caaagagcgc ccggatatga tcgtgctgct
     7381 gctcaccgat gatgccgaaa tgcagagcca cacccagcag ctggaagagt atctggagca
     7441 gttcacagac gatgaaaacg gtgacggcaa ggttcacgta gacgtgtatc ccatccccgt
     7501 tagtgagaac atcaacgata tggactacta caccggaaac gccaccaagc tttccgcaga
     7561 gttccagatg ggtgaggcag tcatgctctt tactgacacc agagccaacg attacattat
     7621 ggcatcggag acgctgactg atctcagcga aaaatatccg ggacatgaaa atattcaaaa
     7681 agacggctat tatctgcgcc atacggattt tgccacgaaa atcgactacc ccggcaatgt
     7741 agaccttgac cttgccatca gtctgcgcaa gccggtcaag acttcggaca gcaaagaaaa
     7801 aatgcaggag acctatgatg tggcagaaaa ggtatttctg cgcatcatgg acgatctgga
     7861 caacaccacc gagccggaag atatcgtcac gaccgaaccg gaaacaactg cggtcacaac
     7921 cgtaaccaca gcaaaggagg attaacgatg ctgcgaatcg gaaaccatct gccctcttcc
     7981 aagggctatc tcgccatggg aaaacacgcc gtgaaactgg gagcaacaac gttcgccttt
     8041 ttcacacgaa accccagagg cggcactgcc aagccgatca acgaggcaga tgtccaggca
     8101 tttctcgcct atgcgaaaga acatgacatc gaccggctgg tagcgcacgc accgtacacc
     8161 atgaacctgt gttccgccaa gccggacatc cgtcagttcg ggctgaatat gctccaggat
     8221 gatatgcagc gcatggagta cacaccggga cagtactata atttccaccc cggcagccac
     8281 accggacagg gcgctgaaac cggcatttca cagatcgccg atgcactgaa ccagacgctg
     8341 acgccggaac agaccaccac cgtcctgctg gagactatgg ctggaaaggg cagcgaagtg
     8401 ggacgctctt ttgaggaact ccgtgaaatc ctggaccgtg tgacactgtc agaaaaaatg
     8461 ggcgtatgcc tggacacctg ccacatctgg gatgccggat acaacattgc tgacgacctg
     8521 gacggcgtgc tgacccagtt tgacaaggtg atcggactgc accgcctgta tgctgtacac
     8581 ctcaacgaca gcatgaaccc ctgcggtgcg cacaaagacc gccaccagaa gctgggacag
     8641 ggctgcatcg ggctggacgc actggtgcgt gtggtcaatc atcccgcact gaaacacctt
     8701 ccctttattc tggaaacacc gaatgaggac gcaggttatg cggcagagat cacactgatg
     8761 cgggaaaaat cagagtgaac aactggtccc gcatgacaaa atgaatcaaa aaaacggttt
     8821 tcatccccat atcttgcatc atttttgaca ttgacttttt tttgaaaatg gtgtatacta
     8881 aaaatgtaca caagggatac accgcatttt tgacaatagg aaaggagcaa ccccatgaaa
     8941 acgatcttgc ttgcaggcgg tgccggatac atcggcagcc acactgcggt tgaacttctg
     9001 aatgccggtt tccaggttgt cgttgccgac aactattcca acagctgccc ggaggcagta
     9061 aagcgtgtgg aggagatcac cggaaaatct gtaaagctgt atgaagtcga tatcaaggac
     9121 agcaaaaagc tggaaacggt tttccaggaa aaccacatcg atgctgtcat ccattttgcc
     9181 ggtctgaagg cagtgggcga atcggtacag aaaccgctcc tctattaccg caacaacatc
     9241 gacaccacgc tgtctctcct ggaatgtatg cagaaatacc aggtgaacaa catcatcttc
     9301 tcttccagcg caacggttta cggagaagaa aacccggttc cctacaccga ggaaatgaag
     9361 cgtggcacct gtaccaaccc ttacggctgg acaaaggtca tgatggaaca gatcctggag
     9421 gatgctgcca aggcaaatga ggcgctctcc gtgatcctgc tccgctattt caaccccatc
     9481 ggtgcgcatg aatccggaaa gatcggcgaa gatccccagg gcatcccgaa caatctgatg
     9541 ccgtatgtgg cacaggtcgc agtgggcaga cgagacaagc tgacgatctt cggacaggat
     9601 tacgacacgc cggacggaac ctgccgccgg gactacatcc atgttgtcga tctggcaaag
     9661 ggtcacgtca aagccatcga gtacatcctg gcgcatgact gtgtggaggt cttcaacctg
     9721 ggaaccggca cgccttacag cgtgaccgaa atcgttgaaa ccttcgaaaa ggtcaacaat
     9781 gtgcctgttc cccatgttta cggtccgaga agagccggcg acctgccgga gagctacgcc
     9841 aacgcagaca aggcactgcg tgtgctgggc tggaaaacag aaaagtctct ggcggatatg
     9901 tgccgtgaca cctggaactg gcagaaaaac aatcccaacg gttatcagaa ataagtgccg
     9961 ggaatatttc ccgtattaaa aaagcggtct ggaagttgca ttccagaccg cttttttata
    10021 ttagagtatg ttctgtatcc gggcatacgc tgactgcgcc gtcagatcgt ccgagaggat
    10081 gctgaacccg ttctcatgag gcaaagccga aattcatccc gtcgtactca tcgtaaacgt
    10141 gagaggactc ttctccgccg tcatgtccaa aactgccgtt atagccgtca ttctggttct
    10201 tctcgtactt gctccactca ttttcccagt tgcttttggg cgttgtcttt ttttcgctgc
    10261 tgtcggattt cgttccattg ttcttttgca gactgcccat tgtggtagtc tccgaacttg
    10321 tcccgttccc gtcagcatca tagccatatc cggcataggg tgcagcggca ttctcctgcc
    10381 cggcacagcc gctcagcaga aatacgccca gcagcgagac caggcaaaat atcgtatgcc
    10441 attttcgttt cattggttgc ctcccaagca ttttcactgc agggcatccg caatcacatc
    10501 cagaatggca tgactcacca tttctttgga ttgcagcggc agttcctgct ccgagtgctt
    10561 tcgaatcagt gtcaccacgt tggtatccgt gccgaacccg gcgccgtcta ctttcagatt
    10621 gttggcacag atcatgtcca gatgcttttt ctccagcttt gccctggagt tttccagcat
    10681 atgctctgtc tccatggaaa agccgcagag gaactgccct tccggcttat gctgccccag
    10741 atatgctaga atatccttcg tccgggtcag cggtatcacc gcatcgccgt cctttttctt
    10801 ttgcttttct gcggcgattt cggtggggcg ataatccgcc acagccgccg ccataatcac
    10861 ggcatcctgc tgtgccgcac gggatgtgac cgcctggtac atctcctccg ctgttgtcac
    10921 gggaacggtt tccgcaaaca gcggcggcgg caatgcagtc ggtccggtca ccagtgtgac
    10981 ctctgcccca cggcgtgcag cgcacttcgc cagcgcatag cccatcttcc ctgtggaatg
    11041 attggtcaga taccgcaccg gatccaggga ttcctgggta ggtccggctg taatcagaat
    11101 ctttttgccg ctgaggtctt tttcacaggc gatttcctgt aagatccact ccagcagcgt
    11161 ttccggctcc ggcatttttc cggcgcccac atcccggcag gcaagcattc ccaccgccgg
    11221 cgcaataatc ttgtacccga aacgtctcag cttttccaga ttgtcctgca caatggggtt
    11281 ctcatacata tgggtattca ttgccggcga gatcagaacc ggcgccgtgc ttgccatgac
    11341 cgtggtagtc agcatatcat cagcgatgcc gccggcgatc tttccgatca catttgcaga
    11401 ggctggtgca atcagcatta catctgcctt tttcgccagg ctcacatggg tcacatcaaa
    11461 ctggaaattc cggtcaaagg tatccaccag gcacttgttc ccggtcagtg tttcaaaggt
    11521 aatggggttg ataaagtttg tggcgttctg cgtcatcagc acatggacat ccgcatgaag
    11581 tttcaccaga ctgcttgcca gtgatgcgat cttataggcg gcaatactgc cggtcacacc
    11641 cagaacaacc gtttttcctt tcagcatttc cccaccgcct tatgccagtt cgccgtgctt
    11701 ttcatagtgt gagattttgg aaacagcgtt tttgtcgtcc accagaacca ccttcggctg
    11761 catggctttc gcctcttccg gcgtcacctg ggcataggac atcaggatca ccttgtcgcc
    11821 ggtctgcgcc tggcgtgctg ccgcaccgtt cagacagatc acgccgctgc cacgctctcc
    11881 ggcaatggta taggtcataa aacggttgcc gttgttcaca tcgacgacct ggactgcctc
    11941 atactccagg attccggcag cgtccagcag atccgcatct acggtaatgc tgcccacgta
    12001 gttcagttcc gcctgtgtca cagtggcacg gtggatcttg cctttcagca tggtgatggt
    12061 cattcagcgg cacctcctgc ctgcatcata aagttgtcga tcagacgggt ctttccgatg
    12121 taaactgcca ttgccgccag gaacggtctg tccagtttcg ggatctgctg catggtcatc
    12181 agatcaacca cctttacata gtcgatcttt gccagcggtt ctgcctcgat gatctctgtc
    12241 attgcctgaa ccagctttgc tgcatcggtt tctccggatt ctgccagctt tttgccggca
    12301 aagatgctac ggctcagcac cagggcagcc ttgcgttctt ccgggctgag ataggtgttc
    12361 cgggagcttt ttgccagacc gtctgcctca cggacaatgg ggcatcccac aatttcaaca
    12421 tccatgttca gatccagcac catacggcgg ataactgcca gctgctgtgc atccttttct
    12481 ccgaaataag ctctgtccgg ctgtacaatg tgcagcagct tggatacgac agtgcagacg
    12541 ccacggaaat gcacaggacg gctcagaccg cagagttcct ttgtcagcac atccatatcc
    12601 acaaagctgc aaaagccctt gggatacatc tctgccggtg tgggatggaa aatcagcgct
    12661 gcgcctgtgc tttcacagag cgctgcatcg tgttccaggt ctctgggata gctttccaga
    12721 tcttcgttcg gtccgaactg agtgggattc acaaagacgc tgacaaccac acgatcgttc
    12781 tgctcacacg cctttgcgat cagactctgg tgtccctcgt ggagatatcc catggtggga
    12841 acgagaccaa cggtcagtcc ctcttttctc catgccttga cctgctgacg tacttctgca
    12901 acggtttctg caatcttcat gcctgtgcct cctgttccag ctgtgccatt acttcatcgg
    12961 aaatggcaaa cgaatgttct tctgccggga aattcccagc ctgtatttca ttacggtatg
    13021 tgacaaatgc gtcctgcatg gtctttccga cctctgcaaa cttccggaca aatttcggct
    13081 ggtagtccat gaacattccn nnnnnnnnng gacctgtccg tcacagcctg cgcctgcgcc
    13141 gatgccgatt gtcgggatat gcagcgtctt agtgacgagc gctgccagct ttgccggaac
    13201 gcactccatt accagtgcaa atgcgcccgc ctcttccact gccttggcgt cctccagcag
    13261 ctttctggct gcctctgcgg tcttgccctg taccttgaat ccgcccaggg tgtttacgga
    13321 ctgcggcgtc atgcctaggt gagcgaccac cggaatggat gcgttgacaa tcgcacgaat
    13381 ctgctcgcag accagcttgc cgccttccag ctttactgcg ccggctctgc cctctttcat
    13441 cagccgtccg gcattgacaa ctgcgtcata caccgaagtc tgataggaca ggaacggcat
    13501 atctgcaacg accagggcat tctgagcgcc tctggaaact gctgccgtgt ggtggatcat
    13561 gtcctccatg gtcacggaga tggtgtcgga ataaccaagg atcacgttgc ccagagagtc
    13621 ccctaccagc agcatatctg tcccggcggc atccatcagc tttgccatgg tgtagtcata
    13681 gcaggtgagc atagcgatct tttcgccacg ctctttcatc gcctgtagtg tctgtaccgt
    13741 ttgtttcata ttttttcctc caaaagggaa tagtacaatt tccgatcacg gaatactgtc
    13801 cagcatttat catagcagaa agagacgaaa aagtcaaggc aatatcgcag aaaacagcgt
    13861 gatacaacat tatataagat tgtgtttcca gccgccgcaa aaaactgctg taaaaaagtt
    13921 ctctttttct tccctttttg cagaaaacgc cgaaaattta caaagcattg acaatttccg
    13981 ccggttctcg tataatggta taagagcctg tcccacacgg tcgggcaatc acaaaaagac
    14041 agcatctctt atgagggaaa ggatacatca tgcaccacat ttggaaaaaa tttgccggcg
    14101 ccggtgcagc agctgctgca gcactggcat tgtcacttct ggcagctcca gtttctgcgg
    14161 ataccgcagc agagatcgtc actgcgccgc cggtggcaac tgcaaccaca cagccggacg
    14221 atacacagca cagcggctgg gaaacaatcg acggcaagcg ctattactac gacccggaaa
    14281 ccggtgaaat gcagaccggc tggctggttc tgacaaacgg cagcatcagc cggagatatt
    14341 tcttcggcga ggacggcgct gtcatgactg gctttttccg gacagacaca aacaccagcc
    14401 cggtctacta tgccgacgaa gagggcgctg ttgtaaaagg cggcattcac accattgacg
    14461 gcaaaccgta ctggttttat gaggacggtt cactgcacac cggatggcag accgtaaacg
    14521 gaaagcggta tttctacgat ccggttaccg gcgagatcac gttcggctgg atcatctgga
    14581 acggcggata ctattacgtt accgaaaaag acggcaaata taccagcctg caggaagtgg
    14641 acggtgagta ttaccccttt gacaaggaaa acggtgcaat cgcagaaggg ctgacagaac
    14701 tgccaaacgg aattacacgt tatt
//
LOCUS       DAWENO010000038        14655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1695_length_14655_cov_11.607945, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000038 DAWENO010000000
VERSION     DAWENO010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14655
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1695_length_14655_cov_11.607945"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5755..5764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3408 a         4188 c         3919 g         3130 t
ORIGIN      
        1 ttaggtgctt atatcggaca taaaggtgcg ggttttgatt tgagccgtta ctttgaaacc
       61 ggtcgatatg attatgatat catggaattc gcaaaaattt acggaacatc gaaagattct
      121 gcctatgtca gtgatgatct tagcgatttg gttaagtaag tttttttgaa ctgaatgtaa
      181 ctccaagagc cgcaggcaca gcacctgcgg ctcttttgca tttttatgaa ttcagcaagg
      241 tcattcttct gtcggcacgt aggagaaccg gcggaacttc gccggcgccg tcatgtccgt
      301 tccgtttccg gaggcataca gcgcaatgta attgccggta aagcagccgc ctgcctcggt
      361 ggtgagatac tgggtctcgc ctgcgccgat ctcctgcatc tccccgtcct ctgccgcaaa
      421 ggcaaaccga taccaatagc ggtcgcagct cagccggagc gttaccgttc cgccgtggca
      481 cggcacagcc ttttccaccg cacagagcgc accgatgcgc cgccgcagga tgatgcagtc
      541 cacgccgtca cgcatggtcc gggcgatctc atagtgatgc cccggattca tgtacacggt
      601 gagacctgcc tcttcgccct cctgccccgg cacaaacgca agctccgtct cggcggtaca
      661 gcagaagtgt tcctgccgcc gtcccagcca cgccttcggc gcatcctccc agagagaaac
      721 gccgttgccg tgcagttcca gtccgtttcc ggtacactgc accattcccg gcaccgggtt
      781 gtagatcaga ttccacatgg ggtgcagcgt ctcgccctgg aactcgtctg tgacagcgct
      841 gcccggtaca tagccggatg ctgcccaggc tttcggcgca tgggcaacgg tgaattccgg
      901 cagaactgcg ccgttttccc cggcatacag ccagccgttt tcccacttcg ccggaacgag
      961 catggtctcc cgtcccagat tgtgccggaa cggatacgcc agcggacgga tgcccaggca
     1021 caccgcccac cagttgccct ccgcatcctc caccaggtcg gcgtgacctg ccgccttgat
     1081 gggcaggctc tgcccgatgt tcgtcagaat ggggttgtgc ggacagggtt catacggacc
     1141 gagaatggac tcgctccgtg ccagcactgc catatggcaa agctctgtac cgccctgtgc
     1201 cagcagcaga tagtacatcc cgttgatttt ataaaggtgc ggtccctccg gattgttggc
     1261 agtcccctgc cagagccagt gagtctcgcc gatggctgcc ccggtggtga tatccatttc
     1321 gctgagatag atgccgccgt ctgtccccag gtaatagacc ttgccgtcct cgtcgaagaa
     1381 cagggacggg tcgattcccg gaaacggcag agcgatgggg tcagaccatt cccctgccgg
     1441 gtctttggca gtgatgataa agttgccgtg gttgtccccg gtcacattgg tgacgatcac
     1501 atagaacagc ccgtcatgat accggatggt gggtgcatag atgttcagac agttgggtgc
     1561 gccggtcacc agatgcacct gagaatcccg tgtcagacag tgtccgatct gctcccaatg
     1621 caccaggtca cggctgtgat agatggggag tccggggaaa tactcaaacg agctggtcac
     1681 cagatagaag tcgtctccta cccggcagat gctggggtcc gggtgaaatc ccggcaaaat
     1741 ggggttctga tactgcataa tttactccgt tctgcccgaa ggcgtttctt ttatccggta
     1801 tacaccgtgt ggttcgccca gtcaaaggat tcgaatgggg tggggttcat gccgaccgca
     1861 cgcatcgcac agtacagcat ggcggcgtag acatcgtccg cctcgatcct gccgtccccg
     1921 ttgatatcca tgcgccgctg cgtttcttcg ctcagcgtgc cgtcgtgcag gatatccttt
     1981 cctgcaccga tccgggcggc ggcgtacatg atctcgaaaa tatcggtgga atcaatgctg
     2041 ccgtcatcgt tcacatcgcc cagcgtgtgc cgtgcaggcg atgctgatgt ggaaaccgtg
     2101 gtcgtcgcag acgttgcggt agtttctgac gctgtgcttt ccgtctgctc cgtggacgat
     2161 gatgacgatg cagattcctc tgcctttgac gtggtcgccg tgcctgtttc agaagaggct
     2221 gtcgtctctc ccgtaacagt ctgcgtcgtg gtcgccggtg cagtctccgt cacggtaata
     2281 tacggcggca tggtcatggt ctccgaacct tttgtggcgg atgccgtggt ctgcgccgtg
     2341 gtgtccgttg tcaccatcgt ggtggctggc ggcgttgtgg tcatgactgt ggtcgtggtt
     2401 gtcgttggag gcgtaagtgc ggtgtactcc agatggaatg cgtccgcata cttctgcaca
     2461 gaagtttctt cgtctcccaa acccgctctg ttttccacca ggatcgcttt tgtcggaaaa
     2521 tagaattcgt ccagattcag cgtacagtga ttatcgtaga aaaataccac cagtgatacc
     2581 cgacaacttt caaaagcata gtttcccaga ctggtcagac tcgccggcag cgcaatatgg
     2641 cgaatacttt tgcagtctac aaatgccttt tcgccgatgt actgcaaatt cgtattggac
     2701 agatcaactg tccctccatc tcccagtgat atacagccat agaatgtgtg atcctcgatc
     2761 tcagtcaaac tatggggaat ggagatctcc accaggtcaa aacaatcctg gaatgcgtgg
     2821 ctgcccagtc tggtcagcgt ctccggcagt tccaccgtag tcagcgcctt acagccgtta
     2881 aaggcactgt ctccgatctc ctccacgttt gtcatgccct ggagcgtttt cagcatggtg
     2941 cagtccagga acgcctctgc ctcgatgcgg cggacactgc tgctgaaatt gacctgcgtc
     3001 agagactcac agcctgcaaa cgctccggga gaaacagatt ccagcccata ggacagggtc
     3061 acatttttca ccttgctgta attggaaaga aatccgctgg cgatcagacg aatgtcgccc
     3121 ggaatgataa tggaagtctc cttgccgtca tacgccagca gcacgccgtc gcagatcttc
     3181 cagtcgtcag tctgtgcctc catccagccg gtgtggaaaa aggcatcctt tccgatgcgt
     3241 tccagcgtct ccggcagctc aaagctgttc agattggtac acatggaaaa ggcatattct
     3301 ccgatggttt tcagccccga cggcattttc agactccgca ggctggtgca gttgctgaac
     3361 gcattgctgt cgattcttgt gacagaatcg gagatcccca catattccag catggtacag
     3421 ccgctgaacg cattgccggg gatctgggtc agactgccct ggatggctgc catactcagc
     3481 gaagtacagt tccggaacac gccttcatac atctgattca gtgatgccgg cagggtgatg
     3541 gaacgcagag cggtgcaatc ctcaaatgcg ccggaaagca gtatctgcaa atgggaatct
     3601 gacccaaact gcgcaacgtt cagggcagta cacccctgaa acgcatagaa tccgatcatc
     3661 cagatactgg cagggaactg gatcgccttg agctttgtgc atccgtaaaa cgcatagctg
     3721 tcgatcacca tcagattttc gttgagagaa atgctttcca gattctcgca ttttataaag
     3781 cacttctccc cgatccgttc caggctttcg ggcagtgcaa cgctttccag actgctccgc
     3841 tccataaaga cgccgtctgc cagccgggtc acgccgtccg ggatggtcac ctgcgccgta
     3901 tccggtgcag ccacaaccac ctttccgccc agcacagcca cttcgtcctg ccgacttttc
     3961 agccatgccg tgttttccat tacatcaaag ccaatgttgt ccagcgactc gccgccttct
     4021 acctccgaca ggttggtaca atttcgaaaa gcatgattcc ccagcgctac cacactggac
     4081 gggatcacca ccttccggat ggacgtttct ccgtcaaaag cacagttttc aatgctgatc
     4141 acagagcggc cgtccacctt tgccgggatc accacttctt tgagggtgtc ctccagaccg
     4201 agaacggatg ctgtggtatt gctgatgcgg tacatcagtc cgtcataggt ctgcaagctg
     4261 tctgcactta ctttccaggc tccctccgga acatacagca gcatagagaa agccatacag
     4321 aacgccagaa aacacgctgc aagtctccct cgtttctttt tctgcatcgc agaaaacacc
     4381 tctttccttt cctaaaaatg gattagtcgt cttcatcatt ttctggatct tccaccagaa
     4441 cgcctgcgcc acgcatggcg aggtaataca tcaattcaaa aatatctgtt gtgtcgatca
     4501 cgccgtcctg attatagtcg gcacaatatg tctgagcggc tgtgatgtca actggtatcc
     4561 ctgcgcctcg tctggcacag agatacatca aatcgaactg atccgatgta tcgactacat
     4621 cgtctccgtt catgtcgccc cgttttcgat ccagggcatg aaatgagcgc tggtatttct
     4681 gcgcatagat ttgcgccgta gaagccgcat atccgttgat acgaacgctg ggttctaccg
     4741 ttttttccgc atcataaatg cggcacgccg ggttcagaat caccaggcgt ttcaaagccg
     4801 tgcagccgga aaaagcgttg gagccgatct cctccaccga ttccggcagc gtgatcttcc
     4861 gcagctgcga acagccgtca aaagcatagt tatagatcac ctggacgccg tccgggatct
     4921 gaagctcttc cagactggtg cagccctgga acagggtgtt gctgatctgt gtcagccctg
     4981 ccggaagaga tatgcttttc agtgaccggc aatagctgaa cccgtggttg cccaccgtcg
     5041 tcaccgtatc cggaatctgt acctcttcca gcgccgtaca ccaatggaac gcatattccc
     5101 cgatggtttt cagatgcgcc gggaactgca cctggtgcag cctgctgcaa ttcagaaacg
     5161 caaaggcatc aatggtctgc accgagtccg gcatcaccac gttttccagg gtggtgcagc
     5221 cctcaaaggc atagccgccg atggtgctta ccgtatccgg gatagtgacc gtagcgtctg
     5281 ctccggtata cgccagcaga aatccctttc cggcgatcac gaactcctct gtctggttgt
     5341 ccagccaggg cgttccctgg acgatgcccc agccgatgtc tgagaccgta tccgggatgg
     5401 aaatgcgctc cagccgggtg cagtccttaa aggcgaactc cccgatcctg cggatggtgt
     5461 ccggcagggt cacctgtttc agccgggtgc agtccttaaa ggcatagcgt tcgatctccg
     5521 tcaccggaca gccgtccagc tctgccggga tgttcgcctc cgtcagtatc gcacgtgcgc
     5581 ctgtgatcgt cacctcgtgc ataggcgtga tggtgtagta gatcatgtcc tggtacacct
     5641 tctgccccgt gtcatcggca aaagccgcaa acggacacgc cgcaaaacag aaaaccagcg
     5701 ccagagccaa cgccgtcaaa agcgacagcc agcgccgcca tatcctgtac aattnnnnnn
     5761 nnnngtacat ccctcctttc tatgtgattt tagaagttgt tcttttaggg cttgtttgaa
     5821 aaatccgtac atttgtcaaa caagccctag aaagaagcat cttttcagca aatttcgaga
     5881 acaaccttct gcatttttat tatagcatag gatggggaat gccgtcaata ttttttcgca
     5941 ttttttccac aatcgtctgc cgctgtccca tcctgcacgc agatatgcaa aaaagcggca
     6001 tgacgaaacc gccataccgc tttcattttg caaattcata gacagaacaa atcctgtctg
     6061 cgagaatccg acgctaactt atgccagttc agcgaagaac ttgatggtgc gaaccatctg
     6121 gctggtgtag ctgttctcgt tgtcatacca agaaacaacc tgtacttcat acagatcatc
     6181 agcgatcttg gaaaccatgg tctgagttgc atcgaacaga gaaccgtact tcatgccgat
     6241 aacgtcagaa gatacgatcg gctcttcgtt gtagccataa gactcagtag cagcagcctt
     6301 catagcagcg ttgatgccgt caacagttac gtctgcgccc ttaacaacag cagtcaggat
     6361 cgtggtggaa ccagtcggaa ccggtacacg ctgtgcagaa ccgatcagct tgccgttcag
     6421 ttccggaata accagaccga ttgccttagc agcaccggtg gagttcggaa cgatgttagc
     6481 tgcgccggct cttgctcttc tcaggtcgcc ctttctgtgc ggaccatcca ggatcatctg
     6541 gtcgccggtg taagcgtgaa tggtgctcat gataccgctc tggatcggag catagtcatt
     6601 cagagcctta gccatcggag ccaggcagtt ggtggtgcag gatgctgcgg agatgatctt
     6661 gtcatcagca gtcagagtgt tgtggtttac gttgtaaacg attgtcggca gatcgttgcc
     6721 agccggagca gagataacga ccttctttgc acctgcatcg atgtgagcct gtgccttagc
     6781 cttagaggtg tagaaaccag tgcattccag aacaacgtca acaccaatct cgccccacgg
     6841 cagctctgca gcgttagcct ttgcatagat cttcagagtc ttaccgtcaa cagtgatagt
     6901 accagcttcg tcatcagcag aaacagtgtg cttgttctca ccgatcttgc ctgcataacc
     6961 gccctgtgcg gtatcatact tcagcaggtg agccagcata gacggcttag tcaaatcgtt
     7021 gattgcaacg acctcataac catctgcgcc gaacatctgg cggaatgcga gacgaccgat
     7081 acggccaaaa ccattaatag caactttaac tgacattgga aattacctcc taatatttta
     7141 gaaagcactg atacacatcc ccggacatct gcccgatttt gccccagtga ttccttgata
     7201 attctattct acaccattcc ggagaaaaat tcaagcactt tcgcaaaaaa agcagggggt
     7261 aaaaaggcaa gttctcttat tttccacgtt ttccgcaaaa aacgggaaac cattttatgc
     7321 aaaatgcaca aaaattgaaa acaagacaaa attatcactt tttttatttc cctgttctga
     7381 aatatgtagt ataataatag catagaataa agacaaatgc ggatgcagtg aggagagatg
     7441 acagcgatgc agcaaagtga atttcagcag atccgggaat ggtatcagca gtttgaggat
     7501 gccgtccttt tctaccgcat ggacggcacg ccgctctggc agaaccacgc cgccaccttg
     7561 cggcaggatc aggacggcgc atccctgcca cggtatgccc cggacggcac gccggagacc
     7621 ggcacgtttt ccgtccggca gggcagtgtg ctgtatctgc tccggcagaa acaatgccgc
     7681 atcggcggca cgccggtgat cctggtgcag atctgcgcac agccggaaga ggaactcttc
     7741 tggtccgacc ccgaccgatg tcacgaggaa gaaaaccgga tcgccgccat acgccagaag
     7801 gtatttggca tctccaacgc cgtcagtgcg ctgtatcagg atctggatga atccagcgac
     7861 cagatcccca ggatgatcct ggaagaacag ctggaacaac tgaacattat tcaaggcaac
     7921 tgctgccgtc tcatgcggtc ttcggtgctg cgtctggaac agctcaaata ttaccaaaat
     7981 tgcgaagtct ccggtgaggc gctttttctg gaccgtgaat tgtcgaattt tgtcgaatcg
     8041 tgtcgaatgg tgcttggcag ggccatccgg atgacactga aaacggcgcc ccacctgtgc
     8101 attttcgtcc accgccggcg gctgcgtgtg agcctgctct gtctgatttt acagctgcgg
     8161 cagactatgc cgtctgcgtc ccatttcacc tggcaggcag agcagcagga aaacgaggca
     8221 gtgctgcgct gtacggcagt ctccgacggc acagagccgc agtctcaccg ccacagcatc
     8281 ccggagcagc tgtatcagac ggggctgcgc acgccggagg aacaggtcgt acagcacttc
     8341 tgccggaagt atcacgccgt cctgctcacc tcccagaccg acacccaggc acagtgtacc
     8401 ctgcgctttc ccctgcaccg ggacatccgg aatctgtccc tggaaagcag ccgccgggag
     8461 atcgacgacg gcatcttttc gctgtatcag atcttccttt ccgacatttc ggactaccgg
     8521 ttttattaaa cagcacattg caacacattc tgtaaagcca ttcaggagtt gccgcctatg
     8581 cgggaattcc tgaatttttt gttaaaagaa gaagataccg tttcacattt tacaaagagt
     8641 atgctataat aggtagagga tattcctccg gacagccaaa aacggcgtga ccggagtaca
     8701 gcagactttc agaaaggaac cgatcccacc atggcagaac gattgattgc aatgcaggac
     8761 aacaaccaat ttcagacttt atgcggcagt tttgacgaaa acctcaagct gttggaacag
     8821 cggtatcatg tgcagatgat ctgccgtggg gacgagctgc gcatccaggg cgacggcgca
     8881 gcggtagagc tggctgccgg agcggtcact gcactgctga aaatgattgc cgccggacag
     8941 gctgtgacgc cgcagtccat ccactacgtt tcggatatgg tggacgaggg cgcaagcgga
     9001 cagctggcgg agctggcaga tgacgacacc gtctgtctca ccgccgccgg aaagccggtg
     9061 catacccgta ctgtgggaca gcgcagctat gtccgtgcca ttgccgaaaa caccatcgtg
     9121 ttcgggctgg gtcctgccgg aacaggaaag acctatctgg cggttgccat ggcggtgcgt
     9181 gcgttccggc ggcacgagat cagccgcatt atcctcaccc gtccggctgt ggaggcaggc
     9241 gaaaagctgg gctttctgcc cggcgacctc caggacaagg tggaccccta tctccgtccg
     9301 ctgtacgacg gcttgtttga aatgctgggc gcagaggcgt tccagcgcca tctggagcgt
     9361 ggctctatcg aagtcgctcc cctggcgtat atgcgtggac gcaccctgga caacgccttt
     9421 atcatcctgg acgaggcgca gaacaccacc ggtgaacaga tgaagatgtt cctcacccgt
     9481 ctgggcgagg gcagccggat ggtcattacc ggagacacca cccagatcga cctgccggac
     9541 aagcagaaga gcggtctggt catcgcctcc cgtatcctgc ggaaggtgga cgacatcgcc
     9601 gtctgcgaat tcaccaaccg ggatgtggtg cgccacaagc tggtgcagga catcatccgt
     9661 gcctacgagg cggcagagcg ccggcagaat agtgacaaaa cagagcgttc tgaccgaaaa
     9721 ggtactagaa agcactaaat ttgaaaacct cataactagg ctcccttgaa aagggagctg
     9781 gcagccgcag gctgactgag ggattttttg taaaatctaa aacaaaacac aaagaaaagg
     9841 catcagtaga atccctccac cagcttcgct ggttcccctc ccttttcaag ggaggcttat
     9901 tcctgttgct gttattgtgt tagacattcc ataatacaat tacatcccct atagaaaggg
     9961 tttgatcgac attgccaaaa aataaaaaaa ttaagatata tgtgaaatct catgaaagga
    10021 gatcgttcat catgccaaaa gtaaaagtca tggtaaaaaa caatcaaaca gaagtacggg
    10081 tcccggtagg aatccgaatg ctcatccgcc gctgctgcca ggcagtcctg gtggcggagc
    10141 atttcgggca ggatgccgaa gtcagcgtct cctttgtcag caacgatgag atccacagac
    10201 tgaacaagat ctatcgccag aaagaccgtg ccaccgatgt gctttccttt cccctgggac
    10261 aggacggcca ctatgacacc aacaaggaga ccggctgcgc actgctgggc gatgtggtca
    10321 tctctctgga aatggctgtc aagcaggcag atatgtacgg tcactccctg gagcgtgaga
    10381 tcggctttct gaccgttcac tccatgctgc atctgctggg ctatgaccac gagaccagcc
    10441 cgctggaaga gcgcaagatg cgggaaaagg aagaggaagt cctgggcgaa ctgggcattt
    10501 ccagagaagc caccttcacc aaccagccgg aggatactga cgcatgacaa aatcggtttt
    10561 cgtgaccctc gccggacaca ccaacgccgg gaagtcctcc ctgctcaacg ccatcatcgg
    10621 cgaaatgatc gcatccgtca gccccaagcc ccagaccaca cgcacacgca tcaccggcat
    10681 caagacggtc ggtgaaacgc agatggtctt tatggacacg ccgggtctgc acaagcccac
    10741 caccaagctg agcggtcata tgctgaaagc ggtgaaggac gctgtggcag acatcgacgc
    10801 cgtgctgttc gtccaggact gcaccaagcc cctttcggaa caggaagagg agctgctgca
    10861 ctctctggaa aaggcgaaaa cgcccaccat cctggtgctg aacaagatcg acctttgcag
    10921 cgacaagacc aggctgatgg ggacgatcgc ccggctgaat gcggtgcatt cctttgccgc
    10981 aaccattccg gtgagcgtca ccgaatcgga cggcatcgac ctggtggaac aggaagtcat
    11041 ggcgctggca cagccggcac cccactattt cccggacgaa aagttcaccg accagccgga
    11101 gcgtgtgctg gcggcagaga tgatccggga aaagatcctg aacctgctcc gggacgaagt
    11161 cccccacggc atcgccgttt ccatcgagcg catgaaagag cgggacgaca aggatctgct
    11221 ggacatcgac gccaccatct actgcgaacg ggaatcccac aaaggcatcc tcatcggcaa
    11281 aaacggcacc atgctccgga agatcgctac cctcgcccgg aaagacctgg aacgcttttt
    11341 ctacatccag gtcaatttgc agtgctgggt caaggtcaag gaggactggc gcaacaagga
    11401 aggtcttatc cacaacttcg ggctggacaa cccggaataa tctgccacac ataaaaaagc
    11461 aataccccac gcaacataat ggtatctcag taaggcggca attttctctg aaaaattgcc
    11521 ctgccctttc caacaggagg tacacaatat ggacgcatgg gatattttct gtatgaccgg
    11581 acggatcgac gattatctgc actaccgccg tggactggac ggcagcgtgc agcagacggc
    11641 acaggcagtg caaaaggagg ttcccacaca tggagatcta tcggggactg gttctggcgg
    11701 aacggagcgc cggagaacgg gggaagttta ttgacgttct cacagagcat aacacggtcc
    11761 gggagatcta tgtccggggc gcaaagaaaa acacctccgc cggactctct gccacccagc
    11821 tgttttccta tgccaatttc agcatccgga aacagcagaa ggggctgtat ctggacagcg
    11881 ccgaacccat ccgcattttc taccgtctcc gggagtctct ctcccggctg agccttgcca
    11941 tgtatttcag cgagctggtg cggctcagcg tccgggaaca tcagaccccg gaggaaaact
    12001 gctttgtgat gcggctgatg ctgaacaccc tccactatct ggagaacggc gagcgctctg
    12061 aggcacttct gaaatcgatc ttcgagctgc ggctcatgac ggaactgggc atgatgcccg
    12121 acctgctcat gtgccgcagc tgtggtgtat ttttaccaaa acggctctat ttctccgtgg
    12181 aagagggctg ttttttctgt gcggactgcg gtgcgccgga gaatctgcgt tctccgatcc
    12241 tgctccgtgc gccggtactg caggcgatgc ggcacattct ctttgcggac tataaccggc
    12301 tgtatcagtt tcaactggga caggaaaatc tccgggcgct gggcgaatgc accgagcgct
    12361 ttgtccagta ccacctgtcg ctgaaaccca gggcgctgac attctaccag tccgttgcac
    12421 acccgtccgc accgcccaat gttacatgat atctttaaca ggagaaactc ccatgaataa
    12481 aaatctggaa attctggaat ttcataaaat cttagaaatg ctggcgaatc tctccgccaa
    12541 cgagatcacc cgtgagaaag tacgcaccat cgtcccctgc tccgacctgg cagaggcacg
    12601 gacagagctg gaaaagacca acgatgcgtt ccagctgtcc gtccagttcg gcgcaccgcc
    12661 gttccacgcc tttcaggata tgcggatggg actgcgccgg gcaaagtccg gggcgagact
    12721 gtcgctgaaa gacctgctgg agatcgccac ggtactgcgt cagattcagt ccctcagcga
    12781 ctggtacgac cgctgctccg gggtggaatc ctccctggac gacctgttca gccagctggc
    12841 gcctgtgccg tatctgctgg aaaagctgga tcgctccatc ctctcggaag aggagatcgc
    12901 cgatgccgca agcccggcgc tggcagacat ccgccgcaag atcggacgct cccggcaaaa
    12961 gctccgggac accctggaca gcatgatgcg caaacaggat gtgcaggact gtctccagga
    13021 cagccgtgtc acgctccgtg acggacgctt tgtcctgcct gtccgtgcgg aataccgcag
    13081 caagatcccc ggtctggtgc atgacacctc tgccaccggg cagaccatct ttatcgaacc
    13141 catcgccgtg gtggatgcca acaacgacat ccgtctgctg gaactccagg aggcggagga
    13201 gatcgagcgc atcatcgagg agctatgcac cgagtgcggc acatgggcag acgccatcat
    13261 ccgtgaccac gaggtctgcg ccgagctgaa cctgtatttc gccaaggcac gcctggcaga
    13321 acagatgcac gcctttccgc cggtgctgac tgacgacggc gtgatcgtgc tgaaaaaggc
    13381 acgccatccg ctgattccct ataaaaaggc agttcccatc agctttaccc tgggcggcga
    13441 aaacaaggcg cttatcatca ccggtccgaa caccggcggt aagaccgtag ccctcaagac
    13501 ctgcggtctg ctgacgctga tggcaatgag cggtatgctc atcccggcgg cagagggcag
    13561 ccagatctcc gtgtttgacc acgtgctggc agacatcggc gacacccaga gcattgagca
    13621 gaacctttcc acattctctg cccatatgcg cagtgtcatc gacattctgg aacaggcaaa
    13681 cgaccgctct ctggtactgc tggacgagct aggctccggc acagacccca tcgagggtgc
    13741 ggcgctggca gaggcagtga tccagcggct gaaggacaac ggcgcaaagc ttatggtcac
    13801 cacccactac caggaactga aactctacgc caccgaacag cccgatgtgc tgaacgcctc
    13861 ctgtgagttc gacatcgaaa cgctccgtcc cacctaccgg ctgatcctgg gatctcccgg
    13921 aaagtccaac gccttcgcca tctgcaaggg gctgggactg tcggacgata tcatctccca
    13981 ggcggagacc ctggtaagcc aggaaaacct gcgctttgaa gaggcagtct ccaagctgga
    14041 agagacacgc aaggcactgg aacacgaccg cagcgccatg cagcaggcac tgcatcaggc
    14101 ggaagaggat gcggcaaaac tccgggaaga ggtacagcgg acacgcaccg accgggaaaa
    14161 cgtgatggaa caggctcggc tggaggcaat gcgcatcgta gagaccacca aggcacagtc
    14221 caacgaaatg attaccgagc tggatcagct gcgccgggca aaggaaaagg catccttcgg
    14281 ggacgatgtg atccgggcaa agggcaacgt caagcgtggt ttccggaaga tgtacgagac
    14341 cgccaatccg ctgaccgaag tcccggacga agaatacgtg ctgccacggg aattgcaggt
    14401 aggcgacacg gtcatgatgg cggataccaa gcagaaagcg gtcgtcgccg gaaagccggg
    14461 caaaaacggc acggtctttt tgcagatcgg cgctatgcgc acgaaagttc cccaggaaaa
    14521 gctgcgtctg gtggcaggcg tggaggaaaa gaaaaagagc cagcagaaac agcgccagac
    14581 cgccggcaga gtctccgcac aggcggcacg gcgtggcacc atggagctgg acatccgtgg
    14641 ctgcacctgc gatga
//
LOCUS       DAWENO010000039        14540 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1716_length_14540_cov_13.797308, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000039 DAWENO010000000
VERSION     DAWENO010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14540)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14540)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14540
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_14540_cov_13.797308"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3332 a         3724 c         4074 g         3410 t
ORIGIN      
        1 ggaggagaac cgccatgcag aatcttaatc gggacgaccg ccgggagatg cgccgccaga
       61 tggtgaattc actggtgctg ctgctggtgg tcttggggct ggctgcggtg tgtgccgtgg
      121 catatcttca cgctagggga ggatttctga gatgaaacgc aaaaaaatga ctttttcgct
      181 gaaaatgctc tgggagggtt ttcggcagtg taagatgatc ggcatttttg ccgctgttat
      241 catcgtgctg ggcgctgttc tggcgccggt ggcacaggtg atcgaaatga gcgccagtcc
      301 gtatcacacc aaggtggtct atgaggcatg gagtgcgaac cccgttatgc tgctggtgct
      361 ggtggctgcg ccgctgatga cgctgatcct gttccacttc ctggacagcc gtgccgcatc
      421 ggacctgtac catgcgctgc cctgtaagcg cattacctta tacctcagct atgccgggtc
      481 agtgctgacc tgggtggtgg tgctgctgtt gctgggaacg ctgacttctt tgatcacctg
      541 cggctttaca cacaattata tcgtactgct gaaagacagc ctgctgccgt ttgcaacaag
      601 catcttctgc atcagctttc tggtggtctc cggcattctg gtttccatga gtatcaccgg
      661 tacagttttc accaatattc tcttttcggg tatcctgctt tttttgcaac ggttctgtgc
      721 ttttgctttg cagaactcta tggtaagatc gctgccgttt ctcacggatt ctatgaatat
      781 gggatttttc cggaatggca ataacctgtt gtttgccggt gtgagcgccg tctttggcat
      841 cacagattcc gggacggttg ccgaagatgt tctgtctccg ggctggcagc cgctgctgta
      901 tacattcctg ctgggactgc tgtacttcct gatcgcagcg ttcctgttct gccgccgccg
      961 cagtgaggct gccggacagt ctgcgcccag ccgtctgctc cagcatatct accgcatcgt
     1021 ggtgaccatg accatctgta tttttattgc ctgcatgctg tatatggaca ttgtaaacca
     1081 caccgcacgg aacggctggt ttatgtatct gatcttgtat ctggctgcgg cgctggttta
     1141 ctttgcatac gaactgatta ccactcggaa gtggcataac ctgctgacgg cactgccggg
     1201 gctgggcatt gtggcactct taaatgtagg cattctgctg ggaatgcaca tggtgcagag
     1261 ccatatcgtg gcacagcgtc cggcaatgca ggagatcgaa agcgtatcgt tctgcaatac
     1321 ggacggcagt gagtatattt cttccagcag ctatctgagc taccaggact atgtcaacct
     1381 ggagtgcctg gacatcgaga tcacagatcc cacggcgata acgctggtgt cctactatct
     1441 ggacgaaaac ctcaagacct gggaggaaag cgcaagtgcc tatgagaaga agtactgcac
     1501 ttctgtttcg gacacttatg aagcgtatgc ggaaagtgac gaatcggctc tgacgaaccg
     1561 caacgatgtt tattatgctt acaatgtaat catccgcacc aaggacaaga cgctgaaccg
     1621 tctgatctat gtgccggcgg cagagacaga aaagctgctg agcgcactgc agtccaacca
     1681 ggcgtatgta gacgcatggc gcaatccgcc ggaggagatc gacggaacgg caacgctcag
     1741 tgaccataca ggcgtttact atctgagcgc agaccaggca gaagaactgc tgcaaagcga
     1801 tcgggaagag ttacagaccg tagattttga acggctgtat cagcaggtgc agaacggcac
     1861 tgtggatgat acctgggtga gcgctatcag ttattctttc cggaagaatg gaaagctgta
     1921 caccctgggc tgccccattt atgagggtat gaccccgaaa acggcgaaga agttttacga
     1981 aatgatctat gaggcacaga aagacgatgc ggcatttctg cgccagctga cggaaaacag
     2041 tgacaaggac atctcgtatt ccatcaacgc atacggcggc gatgatactg cccatgccgg
     2101 atactacgct tttgcatcgg ataccgcaca gctgaacaaa gatgcggcag tacagctgct
     2161 gatgcagtat gtgcaggaca gacccatgca ggcaggcgaa agctatgtga ccatttacct
     2221 gtggccagac gaaggcttgt ccgatgatac acagtcgctg aatcttcggc tgccgctgga
     2281 tgatgcgttt ttcagcgatg aaaagacaac tgacttgttc gacatcgaca actataatga
     2341 ggacggactc atcgacaact ccggcgagga cggactgggc taagttctga catcttcaaa
     2401 gcggagtctg caagtttctc cataagagcc ggaacagact ctgagcagac tcctgcttct
     2461 tccttatttt ccacgaaaag cattctttcc ctgtggggag aggatgcttt ttgcggtttc
     2521 tgatcgttat tgtaacatat gaaacgtgcc gctgtaaagc cgtttggcgc aaaaaatacg
     2581 gttcgtccgt gccgcatttt ctccggaaaa aacttttttt cggggcaaaa tcagagatgt
     2641 cccgaaaaac cgcctgtccc actaatttta ttttcggaga tgatattgcc taaaaatagg
     2701 tgtatttcaa ttcagaaagg caagtttcgc tgttttgaac tttgaaaaat aaaaacctca
     2761 aaaaaatgct tgacagtacc cggaagttgt gctataataa ttatcgttgc agcgcacgag
     2821 tgctggtgta gctcagttgg tagagcagct gatttgtaat cagcaggtcg ggggttcaag
     2881 tccgtccacc agctccaatt ttctacaaca tacatatgtt acatggggga gttcccgagt
     2941 ggccaaaggg ggcagactgt aaatctgttg cttctagctt cgatggttcg aatccgtcct
     3001 cccccaccag agaacaggca agctcttcgg gagtttgcct gtttttcttt ttcgcatcat
     3061 gtgtatggat tgtagagtga ctgaaatact atatcatatg gtaagtacct gcgccgtcag
     3121 ggcgccggtc cgtgacaaca cataccatgg gaaatgagga gaaaattatg gttcgtgagg
     3181 atttgagaaa tgttgctatc atcgcacacg tagaccacgg taagacaact ctggtcgacg
     3241 agatgctgaa acagggcggt gtattccggg aaaaccaggc agtggcagaa cgtgttatgg
     3301 actccaatga tctggaacgg gaacgtggca tcacgatcct ggcaaagaat acatcggtac
     3361 gctacggcga caccaagatc aatattattg ataccccggg acacgctgac ttcggcggcg
     3421 aggtagaacg tgttctgagc atggtagacg gtgcgatcct gctggtcgat gccgcagagg
     3481 gcccgatgcc acagactcgt ttcgtgctgt ccaaggcgct ggaaatgaat ctgaagatca
     3541 tcgttgttgt caacaagatc gaccgtccgg acgctcgtct ggatgagatc gcagacgaag
     3601 tactggaact gctgatggat ctggatgcca gcgaggaaca gctggagaac accccgttcc
     3661 tgtactgctc cggccgtgct ggtactgcaa gctttgaacc ccacggtgag ggcaaggatc
     3721 tgaaaccgct gttcgacacc attatgtctt atgtgaatcc gccggagggc gacccggatc
     3781 agccgctgca aatgctgatc tcttccatcg actataatga atacgtgggc agaatcggta
     3841 tcggcagaat cgaaagaggt accatccagg tgggacagca ggtgctgctg ggcaactatc
     3901 atgacccgga aaacactcgc cgcaacaaga tcgtttctct gctccagatc cagggactcc
     3961 agcgtgttcc ggcagaatcg gcaaccgtgg gcgatatcgt ctggatcagc ggcatcgaga
     4021 acatctccat cggcgacact gtctgcgcac cagaatgctt tgagccgctg ccgttcacca
     4081 agatcagtga accgactgtg gaaatgacat tcagcgtcaa cgacagcccc tttgccggac
     4141 aggaaggcaa gtttgttact tcccgtcaac tgcgtgaccg tctcatgcgg gaactgctcc
     4201 gtgacgtgtc cctgcgtgtg acagagacag aaagcaccga tgcgttccgg gttgccggac
     4261 gtggtgaaat gcacctgtcc attctcatcg agaatatgcg ccgtgaggga tatgagctgg
     4321 gcgtttctac gcctcgtgtt ctgtatcacg aagtagacgg caagcgctat gagccgatcg
     4381 aggaattgtc cattgacgtg ccggaagact gcgtgggttc tgtaatggaa aagctgggtt
     4441 cccgcaaggc ggaactccag gatatgcacc cccagggcag ccgtacccgt ctgacattcc
     4501 tcatcccgtc ccgtggcctg ttcggctata agagtgaatt tctgaccgac accaagggtg
     4561 agggcatcat gagcagcgtg ttctatgaat atgcgccgta taaaggcgat attccccgcc
     4621 gcagcaccgg ttcgctgatc tctttcgaga ccggcgaggc tgttacctac ggtctgttca
     4681 atgcccagga gcgtggctcg ctgtttatcg gcgcaggcgt tccggtatat gagggcatgg
     4741 tcgtaggcgc atccccgaag gcagaggatc tggtggtcaa tgtctgcaag cgcaagcatc
     4801 tgaccaatac ccgtgccagc ggcagcgacg acgcactgcg tctcgtaccg ccccgcaatc
     4861 tgagtctgga ggactgcctg gaattcctgg cagacgatga actgctggaa gttacgccga
     4921 agtcgctgcg tatccgcaag cagattctga gcaaccagca gcgtgccaag atgcgtggca
     4981 agatcaagta aaatcccata ttcccccgtc caagagagga atcaaaatat gaaacatttt
     5041 tggaaaaaga cactttgggc tgcgcttttg acagcagcgt ttgcgctgtc tgccacaggc
     5101 tgcggcaatg acagccagac aagcagcagc accgcaaatt ctgccgactc tgccgcacag
     5161 acagaggaga gcagcgctgc gccggcggaa aacggcggtg cggcagatgc agcagataca
     5221 aacgtgttca cttacgagat gcgagatgac ggtactgtca ccatcaccgg ataccagggt
     5281 gcagaaactg cactggacat tccgtccacg gtggacaact atgtggtctc tgctatcgca
     5341 gaccatgcct ttgaggcaaa ctgggacatc accagcgtga ccctgcccag tggtctgtcg
     5401 gagatcggcg agggtgcatt tatggactgc ggcaatctga cctcggtgac catcccggac
     5461 actgtggcgc agatcgaccg tgccgctttt gccggctgtt cgtcgctggc gagcctgtct
     5521 ctgccggaga cggtctctac cgttatggaa gaggcgttca ccggttgtgc cggtctgacg
     5581 gacttgaccg tggcgaatgc gtctctggag tacgacagct ggggtctggt cgagggcagc
     5641 gagccgctga acgtgacgat cacttgtccg gctggttctg ccattgagac ctgggcagcc
     5701 gccaacggca tcagcacaaa aacgttagaa taaaaaacga taagaaagcg tgggacaatg
     5761 cgggtgtagt tccctacagc atagtcatat ggtatgtagg aatccactcg tgtctgccca
     5821 cgctttttgt tttggaggaa atctatgaaa cacatctgga aaagtgcgct ggctctgctg
     5881 ctggcgctgg caatgaccgc aggcgcattc ggctgcggca gcaagaaaga cgcagaagag
     5941 aaaagcacat cggaaagcgc agcgtcggaa agttccgcag aggagtcggc acagccggtg
     6001 acggaatccg acccggcaga tatggactat cagctgacct atgacaagga caaggtgccg
     6061 gacgacctgg cacagaccat cgccatgtac ttctacgccg tggacaccca gaactatgat
     6121 ctgtacgtga aacagatcaa tccgctgtat cagaccagtc tggaatccct gatgcaggaa
     6181 aagtacggct acggcatgga aaacagcatg gaacagctgc accagaacct ggtgaactat
     6241 gccggctcgg acgatttcac cattgagagc atggaactgg cacaggcaca ggaggttctg
     6301 gcagaggact acgatgcaga caccaacttt gtccaggaat atctcaacgc ctacacccag
     6361 gcgttcggtg aggatttcac caagcagctg gaagaacagt ccgatgccat ctatgacatt
     6421 gcagtcacca tgaagggcaa gaacagcgac ggcgaggaga tcaccattct ggatggactg
     6481 gagatcctgg tggcagagac agacggctcg tttggtgtgc tgggataagc agaatcaggc
     6541 ggaattggtg ttctgtatgg aattccttgt gaaacccttg acaatttcgc cgaaaaagcg
     6601 tataataaaa agtaccgtgt tgcaattttt catgtgattg cggaaaagtt ttggaagatc
     6661 atacacaaga tggaaaggtg tatgtttttt tccacaggag ggtattgaca atgcatagag
     6721 tatacaattt cagtgcaggt cctgcggttc tgccggaaga ggttctgcgt gaggccgctg
     6781 atgaaatgct ggattatcag ggaacaggta tgtctgtgat ggaaatgagc caccgcagtg
     6841 ctgccttcga tgagatcatc aagaccgctg aacaggatct gcgtgacctc atgggtatcc
     6901 ccgataacta taaggtactg ttcctccagg gcggtgcctc tcagcagttt gcagctgttc
     6961 ccatgaatct gatgaagaac ggcgtggcag actatattgt cactggtcag tgggcaaaga
     7021 aggcttatca ggaggcacag aagtacggca aggctgtaaa ggttgcctct tctgaggatg
     7081 agactttctc ctatatcccg gactgctccg atctgcccat tgatgatgat gcggactatg
     7141 tttacatctg cgaaaacaac accatttatg gtacaaaata taaaaagctg ccgaacacca
     7201 agggtaagcc gctggttgct gacgtatcct cctgtttcct gagtgagccg atcgacgtaa
     7261 agcagtacgg cgtgatctac ggcggcgtac agaagaacgt tggaccggca ggcgtggtca
     7321 tcgccattat ccgggaagat ttgattccgg aaaagccggc tgtggagaac acacccacca
     7381 tgctggcatg gaagacccag gcagacgcaa attctctgta caacacaccg ccctgctacg
     7441 gcatctacat ctgcggcaag gtattcaagt ggctcaagaa aatgggcggc ctggaagaaa
     7501 tgcagaagcg caacatcgcc aaggcaaagg tgctgtacga tttcctggac gagagcaagc
     7561 tgttcaaggg tactgtccgc aaggaagacc gctctctgat gaatgtgccg ttcgtgaccg
     7621 gcgataagga catggacgca aagttcatca aggaggcaac tgctgccggc tttgtgaatc
     7681 tgaagggtca ccgcaccgtt ggcggtatgc gtgcatccat ctacaacgct atgcctatcg
     7741 aaggcgtaga aaagctggtt gcattcatga aacagttcga agctgacaac gcttgaggag
     7801 agaatcacca tgaaatattc cattcagaca ctcaataaga tctcgaaggc aggcacagac
     7861 cgtttctgtg atgcttatac catttcggat gatgcgaaag acccagatgc catcctggtc
     7921 cgcagcgcat cccttcacga cacctctttt ggtgacaatc tgaaggcaat tgcccgtgcc
     7981 ggcgcaggcg tcaacaacat tccgctggat cgctgctctg aggctggcat ctgtgtattc
     8041 aacacaccgg gtgccaatgc caatgccgta aaggaactgg tgctgaccgg tctgctcatc
     8101 tcctcccgta agattccgga ggcaatggca tgggcagcaa ctctgaaaga cggcgaaaca
     8161 tctgtggcaa agcaggtgga aaagggcaag agccagttcg caggtccgga gatcaagggc
     8221 aagaccctgg gtatcatcgg tctgggcgct atcggtgcgc tggtggcaaa tgccgcagtt
     8281 gcactgggca tgaaggtcgt tggtactgac ccgttcctgt ctgttggcgc agcactgcgt
     8341 ctggatccgt ctgtgacagt tgtcaagacc caggacgacg tgttcgctgc ggcagattat
     8401 ctgaccattc acgtaccgtt caacaaggac acagagggca tgatcaacgc tgctgccatc
     8461 gcaaagatga aggacggcgt tcgtgtgctg aactatgccc gtggcgaact ggtcaacgat
     8521 gatgatatga tcgctgcgct ggaaagcggc aaggtggctt actactgcac cgacttcccc
     8581 aacgcaaaga cctgtaatgc aaagcacatc attgcaacac cgcacctggg tgcatctacc
     8641 ccggaaagcg aggacaactg tgcaagcatg gctgcggatg agctgatggc ttatctggag
     8701 cagggtgata tccgcaacag tgtgaacctg ccgaacgcac agctccaggc agtttacacc
     8761 aaggtttgcg tcattcaccg gaatgtgccg gcaatgatct ctgccctgac ctctgtcatc
     8821 ggcgtagcag gcgcaaacat tgaaaacatg accaatgcca gcaagaagga ttacgcttat
     8881 accattctgg acattgacgg cgtaaccgac ggcatgatca aagcattgac tgcgatcgac
     8941 ggcgtactgc gtgtacgtgt gatcgaaaag taatctgtgc tgtattgcct ttggcatctg
     9001 cccgaatttt cgggttgaaa cggtgtgaaa gcaccggata gattgcattt ccagagtagg
     9061 aaatggtgcg ttaagtgttc ggcagacggg gagttgctgc cgggacgaaa agcgagacag
     9121 atcgcttgcg gtaccgtttc cgcatcccgc tgaatgtatc ataagaagtc agaagtctct
     9181 cagagaagcc tcttatgtac acgaagcaga aacgtttttg tgttagtaga gagaaacact
     9241 gcgtaaagcc cgcctagcgg cgttatacag tgtttctcaa gaaggaggta tttttcatga
     9301 gagacttttt gtcattattc accgaatccg ccaaggaatt caccaaggtg cgcagcctgg
     9361 tgaccacatc gctgctggtt gccgtttcta tggtaatcga gatgttttcc gttgacatcg
     9421 gctttgcaaa gctgaacttt gcgttccttg ccatcgccat tattggtatg ctgtttggac
     9481 cgtgcatggg tctgatggct ggttttgcct gcgatatcgt gggctgtctg gcacatccca
     9541 gcggcggatt cctgccggca tatgtgctgg tgggcggctt gcagggtctt gtatacggct
     9601 tgttcctgta tcacaaggtc aagggtcaca gcatccagtt cgtcaacaac aagacggaaa
     9661 agtccacgga catcaccctg tacctgcgtg cgatcctggc tcggctgacg gatgtgatcg
     9721 tcattaacct gttgatccag accaagctga acctgcacta cggcttcatt ccccaggagg
     9781 cttatggtgt tgcaatcgtt gcgagagtgg cgaaaaacgt gatcgagctg gcaatcgacc
     9841 tgccgctgct gttcattttg ctaccggtgg cgctgaaagc atatcagcac attttctgcc
     9901 gttcggcagc gccgaaaatc gcaaactaaa tcaagcgtta aaataatgcg aaaatagaga
     9961 aagccgcctg cggctgtccc tttgctctgg aagggtacag ccgccaggcg gcttttctta
    10021 ttggtagatg ttctctgcat ccgaatacgt ctgttcccgg agctgctgcg tcagcgctgt
    10081 cagaaacggt cgtgctgtct cgccgcagtc atagactgtg ccgttggcat tgcggcagca
    10141 gccgtccttg agcgaaaggc gcacagcgtc tccggagaag tccagggtca cgatataaga
    10201 tgccgtgccg tccggattct gattgctgtt ttcccgatag aggtgaatct cctgcaagcc
    10261 ctccagcagg gcagtgcagg cggcgttgtc cgtaaaggtg tagcagtcgc cgctgccgtt
    10321 gtccgtgacg gtgatgtcag agagtgtgtc ggctcggagc gtggatgcga tcagcgtggt
    10381 atacggcggc tctggttcgg acttttcctt gtggaaaaag agaaaataaa cgcccagagc
    10441 gataccgccc agcacgcaga tgcccagcag gatcagcgca ccgattttca gatgcttttt
    10501 gcgcagagtc atgcgttttc ctcatccagc cgctttatca tttgcagata gttcagattt
    10561 tcgtagccca ggcggtagta gagttcctgc gcacgctcat tttccggttc ggtctccagg
    10621 cgcagcatca cagcatcgcc ccggagctgt tcctccagcc atgccagaaa acggctgccc
    10681 agaccctgtc cccggtatgc cgggcggatg tacaaatcct cgacccagac catcacgccg
    10741 cctgcctcgt gctgcatcat gcggttggtg accgcaaagc ctgcggtgtt gtcaccgtcg
    10801 gtgaagatat agccggacag atactgaccg gaacgcatca tttccgaaaa ggcacggcgg
    10861 tgatattctg ccgggatggg gtgcaggaca gcgtcggaat ggtaaaattc ctctgccatg
    10921 gtgtagaagt ccgtttcgtc tgccgcagta atgggacgga atgtcagttc gttcatcggt
    10981 gtccacctcc tccgccgtgg ctgccgccgg aagaactgct gtgactgctg ccgccgcctc
    11041 caccgccgcc gccgctgctc tccgaggaga ttcgggtgcg agtagtgtag gtgcgcataa
    11101 actggtcgga gcgttcaaga tactgcacat cgttggtgac cagacatcgc tgtaaactgc
    11161 cggcagtctt gaactgatac cgtttccgga tgctcagcag ggcaatgaga aatgcgatca
    11221 gtcccactgc aacggcgagg acgatgatcg ttccccagct gaatacggcg gtatattctg
    11281 acggcagttt atccacctgc tttacctggt cgtcctgcaa aatgaggtac tttccggtac
    11341 tcttgttgta ggtgtagtat ttgtcagagg gagcgccccg atcggcgtag tattccagat
    11401 cgtgacaaaa gctgtggatc gcagacggca gatcctcgtt tccacgctcc agatattcct
    11461 ctacgtcctg aatcatagtg ctgacacggt cataatcttc gccgttgctg tagtagaact
    11521 gtcccttgcc gctggtggag agatagtcgt atagggaata tgctccggac atatccagat
    11581 agaggaatac gccgtcggtg tcgatgccgt agagttcttc gtaggtatcg tcgcagaatg
    11641 caacggtatc ttcgtcagag ccgtctccga tctcggtatt gccgatccac aaggcgacgt
    11701 tcatgccgct ttcatctgct gcgttctgga gttcctccat tgctgtctgg aattcgctgt
    11761 ccgacagaaa atctccctcg tcacagagat agacattgct gctggaatcc tcggttttcg
    11821 ccgagacgga gggaaacgca aaattgcagg cacagatgcc ggcagccaga gccgccgctg
    11881 tccatttcca aaataatttc atcggaacaa ataccctcct aacagtattg caagtcctat
    11941 cacagcagcc gctatcacgc cgctgcagat cagcgccttg ggccaactga gcggcggtgt
    12001 gcctgcctgt gtgccggtct gaccgttcat ggcgaagtcg tagtcctttc cgtggtactg
    12061 gtagtgcatg aaccagactg gcagcaggac gtagtcgtgg cggatgttct ccatctggac
    12121 gttgtgcgag gtcaccacgt gcgagctgta accggtgatg gagcgttcca ctacctttac
    12181 ggcgtgggtg tcgatgcgct tgtggatccg tggcaggatc tggtcatacg gcacgtcgta
    12241 tttctgcgcc ataaagccgc tgaggtactt catggaaaag ggtttcatct tatcgtagga
    12301 aaacggctcg atggcatcca tcagcgcatc ctcgatcttc tgagagccgt ctgccggtac
    12361 gccacggtag gggactttcg ccagacgctt gacggcatat tccttggtct ctgtgacacg
    12421 atagtcaccc tgccgccagc tgcgctcctt tttggcgagc gctgtcacat cggctttggc
    12481 gtcgcagttg gtgagccaga acggtacata gacgccggtc atcttttcca gctgcttttc
    12541 cgaagtgaaa cttttgggca gaaaccagcg ctttttgcac cagtcgtgga actttttcac
    12601 cgccgcatcc cgtgtgactg caaagggcag cacatatgcc ggcttgtatt cgcctgtgag
    12661 ccgtccggcg agaaccaccg gggcatggca gtagtagcag aatgtggcgg aagtctctgc
    12721 ctctgcaatg atctgtgcgc cgcagttctg acaggtgtag agacgtgtct cgttttcaaa
    12781 cgccttgtcg tcgttttcgc tttccagcgt ctccgcagcg gcatccgcat tctggttcgc
    12841 ctgctgttcc atgttctggt acagctgctg catttctgcc tcggtgtagc tggtgtcgca
    12901 gtattcgcag ttgaaattct gatgctccgg gtcgaaagtc agctctgcgc cgcagttggg
    12961 gcatttatat tgtacggtat tcatgttttc tcctttgtcg gcacttatcc acgcttgctg
    13021 ccgcagttgg tgcagaagtt accggtattc tctgtgccgc agctgcactt ccatgcgccg
    13081 gacggctccg gtttcttgct gccgcagttg gtgcagaaat tgccggtgtt ctctgcgccg
    13141 cagctgcact tccaggtgcc ggcaggcgca ggctttttgc cgccgcagtt ggtgcagaag
    13201 ttgccgctgt tgactgtgcc gcaggtacag gtccagccgt ttgccggagc agcctgtgcc
    13261 gctgcctgct gctgcatctg ctgtgcgttg gtggcagatg cgcttgccat aaagttgcct
    13321 gcgccctgca ttcccatgcc cattgccatg tacgcctgcc ctgcgccggc acggttcgag
    13381 ccggcagact gcaagccctt tgccacagcc gactgcacaa agccctcacg gacgctgggg
    13441 tcacccatca tagcgccctg gttgcgcata ttgatgagcg ccttgctctc gtcgtcgtag
    13501 ctgatgctgg cgatgcccac ggcgcagata tccatgccac gtgcctgtgt ccagtcctcg
    13561 tccagaacag ttgccatata cttggacagt tccatgctct tggaaggcag ggcagagatg
    13621 cggacaccgt ccacggacat ctgaccgatg gcagactgga gtgcggtgag gaattccatg
    13681 ttgaactggt cgcccagggc atcgaatgtg agattggatg cgttgcgtgg tacgacttcc
    13741 atgaagaatt tcagcggatc ggtgatcttc acggaatagc tgccgtggca gcgtacgaac
    13801 agctctgcgt tatagaagtt gtcgaagtat tgcagggcgt tgcgtgtgcc gaacttgatg
    13861 ccacggatct cctgcaagtt gatgaagtag acctgctggg acttgcccgg ctgaccgccg
    13921 aacttaaagc gctcgaagga ttcccggacg gaatctttca gttcgccgtt gaacattgac
    13981 ggagaagagg agaggaacac ctcatagcag ccctcttcgg cagtgtagtc cacgatgcgg
    14041 ccgctgtcca ccaggatcat catctggttc ggacctacct caatgcggga gccgttggaa
    14101 atgatgtccg gcgttccctt ggtgttgctg ccgtgtttgc cgcgcttgat ctgtacccct
    14161 cttgtcagaa cggtgtttgc gcccatgtcg ccggctgtga tatattccag ccaggaatct
    14221 gccagagtgc cgcttactgc acctgctgct gcacgaatca aacccataat ataaacctcc
    14281 agttgtattc tctgcgggac gaaatgctgt cccggcgttt tgtttcgagt atatgtggtt
    14341 tctgtcgctg aatcgcaaca ggaacaacac aattatagca taaaaagatt taatttgcaa
    14401 gagaattcag aagaattgcg aaaaaaacag ccgctgaaac cagcggtttt tggaaggttc
    14461 gcatgggaca aagcctgccg gatgtccgaa ttgaaaaaac gcttgacttt ttcggaaagg
    14521 tatggtatac tgaaacatat
//
LOCUS       DAWENO010000040        14412 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1739_length_14412_cov_10.390054, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000040 DAWENO010000000
VERSION     DAWENO010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14412)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14412)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14412
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1739_length_14412_cov_10.390054"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2559..2568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4163..4172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13620..13629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3106 a         4075 c         3565 g         3636 t
ORIGIN      
        1 catcttctca tactttttca tattcatgga gtctacgccc agttctgtgg aacggacagc
       61 cttcatgatc gcctcttcaa agctggtgcc gatcgccata acttcaccgg tagccttcat
      121 ctgtgtaccc agtgtacgct ttgcatagac gaacttatcg aacgcccact tcgggtactt
      181 gacaaccaca tagtccagcg ccggttcaaa gcaggcatag gtctttccgg taacctggtt
      241 tacgatctcg tccagtgtat agccgatggc gattcttgca gccacctttg caatcgggta
      301 accggttgcc ttggatgcca gtgcggaaga acgggacaca cgagggttaa cctcgataac
      361 tgcatactcc atgctggtgg gatgcagcgc aaactgacag ttacagccgc cttctacctt
      421 cagttcggaa ataatgttga tggcagcggt acgcagcatc tgatattccc ggtcagtcag
      481 cgtcacagtc ggagcgataa cgatgctgtc accggtgtgt acgccaaccg ggtcaaagtt
      541 ttccatggag cagacggtga taacattgcc cttgcggtca cggattactt cgaactcgat
      601 ttctttccag ccgctgatgc acttttcgat cagaacctgt gtgatcggag acagacgcag
      661 accgttcttg gtgatgtcac gcagcatttc ttcgttatcg gcaataccgc cgccagtgcc
      721 gcccagagtg aatgccggac gcacgataac cggatagccg atggtctttg cgaaatctac
      781 agcgtcttcg atggtctcta cgaccttgga cgggatgcac ggctggttga tcttttccat
      841 ggtgtccttg aacgcctggc gatcctctgc cttatgaatg gtcagcggat ctgcacccag
      901 cagcttgacg ccatttgcct ccagaaagcc ctcttccgcc agctgcatgg aaagggtcag
      961 accagtctga ccacccatag tggacagtag gctgtccggc ttttcgatct ggatgatgcg
     1021 ctttaccaca tccaaggtca gcggttcgat atataccttg tctgccattg ccttatcggt
     1081 catgatggta gccggattcg agttgatgag gacaacctcc agaccttcct gtttcagcgc
     1141 acggcaagcc tgtgttcctg cgtagtcaaa ctcagcagcc tgtccgatga tgatcggacc
     1201 ggagccgatg acgagaacct ttttaatatc acttctcaat ggcataactt acttcttctc
     1261 cttttccatc acagcaacga attcatcgaa caaatatgcc gtatccagcg gaccgccgtg
     1321 tgcctccggg tggaactgca ctgtaaaggc attgatctta tgatagcgga tgccctcaca
     1381 ggttttatca ttggcattga tatgagaaac tgtaccgatc tccggatcca ggctctcacc
     1441 cacaactgca tagccgtggt tctggctggt aacataagtg cgatggctgt tcaagtcaat
     1501 aaccggctgg tttgcgccac ggtgaccata cttgagcttt tctgtccgac atccctgaga
     1561 gagcgccagc agctggtgtc ccaggcagat gccgaagatc ggaatgccca gctttgcaat
     1621 ctcgcccacg ttgtcgatga tcgcctggtt ttcagacggg tctccagggc cattggaaag
     1681 cataatgcca tcgatgtgca ttgctttcag ttcctctgcg ctggtagttg ccggaagtac
     1741 gacaacttcg cagccacgct tcaccagttc attccggatg ttgcgcttat agccgaagtc
     1801 gtacagtgcc accttgtact tcggtgtgcc ttcgctgtag gttctgggtt ctgtaccggt
     1861 tacgctgaca acagcgttct tgatggtata cttgcgaacc tgttccagca gttcttcttt
     1921 cttggcgtaa acatcttctg tggtaatcac gccattcata acgccgacct cacggataat
     1981 ccgggtcagc ttacgggtat caatgttata gatacctaca atgttctgct ccttgaggaa
     2041 accgtcaatg tcgccctcca tacggaagtt cgacggtgtg ttgcaccatt cccggacaat
     2101 gtagccgtga atataggact gtgcagactc gctgtctgct gcattcacac catagtttcc
     2161 gatcagcgga aacgtctgtg tcaccagctg tccataataa ctgggatcgg tcagtgtctc
     2221 ctgatatgcg gtcatactcg ttgtaaatac gacctcaccg attgcagttc ctttgacacc
     2281 aaaatgcaag ccctcgaaaa ttgttccgtc tgccagaagc agataggctt tatcgcctcg
     2341 ttccaataat gacatgacac cagtcctttc caacctgaat aaatttatag atatgcggtg
     2401 atatcatcac gcatacggat tgcttctctt ctcgctgcct ctgcaaatgc agtctccggg
     2461 cagtttcctt ccttcttcca tgcacacatg atagcacggg aagagtttac gatcgcacct
     2521 ctgccgtttt tatcaaatgc accggcaaca ccctgtgcnn nnnnnnnntg cgccgccgcc
     2581 ctgtgcgccg tatcccggca ccaggaacat ggtgtgcggc agtttctgac gcagttcagc
     2641 cagctgttcc ggataggttg cgcctacaac tgcgccgaca gcggaatagc cgtaggcacc
     2701 gatctgcttg ctgccccagg attcgcacat ttcacccatg atctcataga ccggcttgcc
     2761 gtcgatctgt ctgtcctgga gttctccgct ggacggattc gaggtctttg ccagaacgaa
     2821 gataccctta tcatacttct cgcacacatc cagcagcggc tgaatgccgt ctgttcccag
     2881 atagccgttt acggtcagtg cgtctgcgcc aaaggcttcc agctccacgc cgtctacctc
     2941 tgtcttgccc aggtgtgcag ttgcatatgc agtcatagtt gcgccgatgt cgttgcgctt
     3001 gccgtctgtc atgacaaaca ttcccttgct ctgcgcatag cggatggtct ctgccagtgt
     3061 gcggacaccc tcccagccgt acatttcata gtaagctgcc tggggcttga ttgccgggac
     3121 aacatcacac agtgcgtcga tcagtgcctt attgaactga agaatcgcat ctgctgctgc
     3181 ctccagggtg tgtccatact tgacaaagct ggcatcccgg atgtactgcg gcacgtattc
     3241 cagacgggga tccagtccgg ctacggacgg attgtgcagt tcatcaattc gctgaatcag
     3301 tctatcaaaa gccatttgca tctattcctt tcccttgaca aatccactgt tacttgcggt
     3361 ttgccatata ttctgtatct tgatagcgga tcacgccgtc tgtgatggta tacaccggtt
     3421 ttcccaccag tgtcttgccc ttccagatca cgttcttcgc tttggagtgc atcttttccg
     3481 gatcaactat ccagtgatag tccagatcga ccagagtaat atctgccggc tcgcccactg
     3541 caagcttatg ctgcggcaga cccagaatcc gtctcgaatt ctctgccatt ttctcgacca
     3601 gctggggcag tgtcagcatt ccggcgtgta ccagcttcag cgatgccgcc agagaggttt
     3661 ccattccgat cacaccgttc ggggcatgat agaagtctgc cttttcttcc ggcgcatgag
     3721 gtgcatggtc tgtcacaatg cagtcaattg taccgtccag cacgccctcc agcacagcct
     3781 tgcggtcttt ttccgtccgg agcggcggat tcatccgata gtctgcatcg ccgctgagta
     3841 cggcatcttc cgtcatgagg aagtagtgcg gacaggtctc acaagtcacc tttacgccct
     3901 gtgccttggc atggcggatg atctccacgc tgccgtaggt gctgacgtgt gcaatgtgaa
     3961 tcggcacatt cgccgatgct gccagaatga tctcacgggc tgtgatatag tcctcggatg
     4021 cacggtccat accctttacg cccagtgttt ccgatgcctt gccacgattt acgatgccgc
     4081 cgtcaataat ggacagatcc tcgcagtgag agatcaccgg gatgcccagt tctgctgcct
     4141 ggatcaatgc cttgcgcata gtnnnnnnnn nncatcggaa atggcgcaga tgcctgcctc
     4201 tttcagagcg gcataatcgc acatttcttc gcctttcagt cccttggtaa tgcagcccac
     4261 cgggtgtacc tctacgccgg tatgggatgc cttttcgcag atataccgaa cggtatccac
     4321 gctgtcgatc gccggtgtgg tattgggcat acacgctacg ccggtaacgc cgccggcaag
     4381 tgccgcagct gcgccggtga agatgtcctc tttatgggtc tgacccggat cacggaggtg
     4441 aacgtgcata tcaaacaagc ccggcagtgc cgtcagacgg gaaccgtcga tcgtcagccc
     4501 tgccgcatcc ggcaagtccc tgccaaactt tgcgatcacg ccgtcctgaa tcagcatatc
     4561 gcatctcggc atcacgtcgc cctgtacgac ctgcacattg cgaatcaaaa tggtctgttt
     4621 cattctttcc tcatttctgc atcggtgttc tgataaatca caacacaatc tgttccgtct
     4681 atctcctgca cagagacctg taccgtctcg ctctgcgctg tgggaacatt tttcccgata
     4741 taatccggac ggatcggaag ttcacgatgg ccacgatcca ccagcacggc aagctgcata
     4801 cactggggtc tgccgatcgc cagaatggca tcaatggcag cccggcagct ccgtcccgtg
     4861 tacaaaacat cgtccacgat caccacacgc ttatcctgta ccggaaaatg aatggtactc
     4921 cgggcagtgt gggtggcatc ggcgatgcgg tcatcccgaa acggcgtgat gtccagtgcg
     4981 ccaaattcta cttcgacctg ttccagttcg taaattttcc gggcaatccg ttccgcaagc
     5041 acaacgccac gggacagaat gcccacgata catagatttt ctgcaccacg attgcgttcc
     5101 agaatctcat atgtgatccg ggcaaccgca cggcgcatgg cattttcgtc catgatgact
     5161 gctttctgtt ccatacacac ctcccgatct ccgcagtaac ccctgtctgc acatggcagc
     5221 aggttcaaaa aaagcactca cccgtttttg acaggcaagc gcttacacct tttgcatcca
     5281 gcgaatgcaa aagaatcata tccgattgat gaaacgcctt gtcaacctca cgggatcaac
     5341 ttaaaggaac cgctcataag catatccgca gacatacttc ttgattgata ttagtatacc
     5401 acactttccg gtgcttgtcc agtgtttttt tacaatttcc gaaaagttta cactttttct
     5461 tccccggaac tgtccttctc ggaaagacag ttgctgagcg ccaccagctg ttccatggtc
     5521 agattctcca tgcggactgt ttcggacagc cccagttccc gcagcgcgtc cagcaaaacc
     5581 ggcttttcgt agtgcatggt tgccgaaagc gcattcacca gcgtctttct gcgctgggaa
     5641 aaaccgtttt tcagcatggc gaaaaagtgt ttcggatgtt tcgtatccgc tgcatagcgc
     5701 ttctgcacat caatgcggat gacggcggaa tccacattcg gcggcggcag aaagctgtct
     5761 cttggcacgg taaagagcat ttccacagtg ccgtagtagt tcactgccgc tgtgatcgca
     5821 ccggaatttc gtgttcccac cggtgcctgc aatttctcag caacttcttt ctgcaccatg
     5881 atggtaatgg agtcgatggg cgcaccgctt tccagcaggt gcatcagaat cggcgtggtg
     5941 atgtagtacg gcagatttgc gcagactgca acgtgcattc cgggaaattc ctgctccagc
     6001 agctgcggca catcaaactg cagaatgtct ccctggagaa tgtgaaggtt gtcgaactcg
     6061 ccgactgtct cttccaggat cggcaggaga cgctgatcca gttccacaga ggtaactttt
     6121 tccgcacgca gcgccagctg ctgagtcagt gtgccgatgc cgggtccgat ctccagcacg
     6181 ccaaatcccg gcgcagcgtt tccctcttcg gcgatgcgtg ggcagacaga cgggtcaacc
     6241 agaaagttct gtcccagttt cttggaaaag tggaatccgt gacggctcag aatcgaccgg
     6301 actgttttca tatctgttaa atttatcatc atggttttac ctcaatactt ttttgaaaat
     6361 gcgtactgta cagataacac tctttgaccg gcattcctgt gatgcactgc agtgcgccgc
     6421 agtaaagcat cagctgttcc cggtagctgt ccaccagcac atccggcgaa gaaacggtat
     6481 ctgttttata atccaccagc acaaagtgat ctccttcctg gaatgccagg tcgatgatgc
     6541 ctttcagaat gctgtccgag ttttcatagc gccgcaggat cgccgccgtc tccggatatg
     6601 cactcagatc atggcactgc accaggaatt tttgttcccg gagaacacgt ttcgagcggc
     6661 agagacggcg gtacaggtcg tcccggaaaa acgccgccgg gatctccggc tttacagctg
     6721 ccgcctgttc cggggtgaga aagccatagg tttgcagacg ctggatctct gccgggacat
     6781 cagcctgcgc tttgcggaaa tccgcatact ggaaaaaggt gtggatcgcc gtaccacgct
     6841 ctgcaccggt cagatgaccc tcctgcaagc ggaaatccgg acgtttccag acgggcgcac
     6901 gtttttggtg tgcctcctgg agtgcggaaa cactcagcag ggactgtctg ccgcaatccg
     6961 gtgaagtata ctgaaaggca gtgatttttt tcaagtcttt cagcgtatcc gcatccggct
     7021 ggataaccgc ctgttccggc agtgtgtcgc tctttgttac cggcggcaaa gaaaattcat
     7081 agtggatttg cagtttttcg ctccacgccg gtgtgttcca gcactgcggc ggcagattga
     7141 tctctctcgc cagtactgca tcatgccgca gcaccaggca catccagacc cagtgcgcca
     7201 tagaaccggc gttctgcact gtcagcggcg gcagtgtgcc gtccgggaga atcagtgtgc
     7261 tgtactgtgc caggtaatcg gtcgttctgg tccgcacttt tttcagattc agcggcagga
     7321 acagctgctg cttggctcgt gtcatggcaa catagagcag ccgcagttcc tcgctcctgg
     7381 aagcgtttcg gttctcctgt tccaggacag caaaaggcat ggtctttgca cgggtgtaat
     7441 tttccgggtt cttgatgcag aacccgacca gtcccttttc ggagaacacc gcatttttcc
     7501 ggctgtcctc ggtggaaaac ggagattcca gcttgcccag gaatacaaag ggatattcca
     7561 gtcctttgga acggtgcatg gtttttacgg caacagcgtt ttccacccct gcggtcagcg
     7621 aagtctgctg aaaatcgttt ccgctgtcca gcagccagtc gatataccgc aggaaaccgg
     7681 atactccatt gtgtgccgca tccgtgttct cctcgtactg tttggcatat tgcagcagca
     7741 ggtgcagatt ggcacgcttt cggtcgccgt cctttgtcag ctgcatgaca gacaaaaagt
     7801 ctgtggtatc gtagatccgg cgaatcagcg cctccagtgt catcatgccg gacgactgcc
     7861 gcagcttttg cagagtatca tacagctggc ggcatttctg caccagaaca tcgtccagct
     7921 gcaccggttc ggcggattcc gttacaccga ttgccatgca gaccgcactg taaaggctgg
     7981 tcttttttcc aagcatccgt atccgcatca gatcttccgg cgtaaaccag aacatcgggg
     8041 aaagcatgat tgccgcaagc gaggtgtcca atgtgggatt gtccagcaca cgcagcatat
     8101 ccagcagcag ccccacttcc cgtgctttta gataaccggt ttcgtccgga ctctgcaccg
     8161 ggatgcctgc ctgctccagc gcatgagcaa agctggtaca catggggttc gtccgcagca
     8221 ggatgcagaa atccctgtac tggcagggac gtgtggcatg gtctttctgt gcaactggca
     8281 ctccggcaac gatcatttcc tggatctttc cgatcacata tgcgatctgc acatcggtgt
     8341 ccgcatcgtc ctcgggcgtc agaactgcca ctgtgacttt ctgctgttcc tccggcagaa
     8401 tttcggtaat atccgtgccg ggatagagcg cctcagcatc ggtgtatgcc acatcgccgc
     8461 aggtatctga catcagactg ccgcagacaa agttgacaaa atccagcaca gccggaacac
     8521 tgcggaagtt ccggttcagc acaatgtgcc ggcagacgct ggtttcggtt ccggcagacg
     8581 aaatggcgtg gacaaagttc tggggatttg ccagacggaa acgataaatc gactgcttga
     8641 catcgccgac gagaaaaaca ttgtcgccgt agcgaagcgt cccggtctcc ggctcgatgc
     8701 agttccggga gagcagcttg aaaatatcat cctgcttatt gttggaatcc tggtattcgt
     8761 cgatcatgat gagctggtaa tacgcctgta attcttttgc cagcgcagag ggctgaattg
     8821 ttccttccgg tgagagttct gccagaagtt ccagcgccat ccgctctgca tcgtcaaacg
     8881 ccagggcatt ttgctgcact ttttgctgcc aaatctctgc cgtcagctgt tcttccagtt
     8941 ccaacagcag cggcatcagc tgtgcgtgct gtaccaggtc gcctgcctca taagggtaca
     9001 ctgcctcaag aagtgcagcg atttcctttt ccagtgcagt gtactgctcc cgcagctttt
     9061 tgacacgttc gaacgcctcc aggtctgtca ttgccttttt cttacgtgga aaattacccc
     9121 ggttctggtt tttttcctgg atgggcgcaa gaatcttttc cgcatccggt tcctgctgac
     9181 gcagcatttg cagcaccttt tccaggcagc gaacatctgt ctcgacccag ggcaggacat
     9241 tgttgtccgg cacggaatcg tacacgacag ctgccaggtc tgccgcctcg tttgccaggc
     9301 gcacggcagt ctcggcacgc cgttccagct gtgccagaaa cgccctatgg tatgcagaat
     9361 cggacagcgg ctggttcagt tcctccagca cctgttttgt ccactgctcc cggaacggca
     9421 cagagccaag aaagctgtgc agttccagca gcagttcttc cagcggcttg tcatttttct
     9481 cgcaaaagca gttccacagc agctgcattt cttcctgccg ttcagcgtgc cagcgattca
     9541 gcacggtatc ggctgctttg gaggcgatca gctttgcctc tgtctcgttg aggatgcgga
     9601 atccggcagt gattccctgc tcggtcagat tgtcccggat caaatcaaaa caaaaggaac
     9661 tgatcgtaca gatatgcgca ttttgcagac gcacctgctg ctgatagagc cactggtttt
     9721 ccggctgttt ttgcagctgg tcgtccagcg cctggtacag acgggagcgc atctctgccg
     9781 ccgcatcgtt ggtgaatgtt acaacgatca tccggtcagc cgggacacgg tgttctggcg
     9841 tttcgtcctc cagaatccgc agcagacgct ctaccaaaac agaagttttt ccgctgcccg
     9901 ccgccgcaga aacgatcagg ccgcagtctc tggttgtgat tgcctcactt tgctgttctg
     9961 tccatgccat cagttgttct cctcctctgc cagttcttcc aggatctcca tcatcttttt
    10021 ctcctgcatt ctgctgtctc cgtatggtat gcgctggcgg caaagttctg aattgccgca
    10081 gatgttggaa aaagcgcaga atttgcacgg gtcttccttt ggcagttcca ggggcagcgc
    10141 atcgatctca ccgtgataca cacgctccgc catatccacc agctgttcca gcacatattt
    10201 ccgcagattt cgcatttgct cctgcgtgag aaatgttccc tttttggtgt ccagttctcc
    10261 tttggcatcc agctttccag gaacgtaaat accctcgccg ttctgttcca tcagcgtcag
    10321 caggtgcgca tcccggagca gcacgccatt catctggtaa gtctcgttcc gctttttctc
    10381 cggcgttttc gatgcacccc gtttctggtc gcttttcacc ttgccagccg gaagatacag
    10441 tacaccagcc ggctgggcat cggacagcgc cgttccctcc gacaaaatgg aaaacagata
    10501 aatcagcatc tgcatatcca gaccatagag cagatgcccc agcgaaaaga ccttttcccc
    10561 tgtcttatag tccaccacac gcacccattt ctgtgcgccg tcccggcaga tatccacacg
    10621 gtcgatcttt ccgataagcc gcacggtatg tccgtctctg gttttgaggc agatcggcgg
    10681 aaaatccgga ttgccgggtg tgatctgaag ttccgtgcaa tagggagaaa aggcagactg
    10741 ccggaactca tcctgcagat gcagcagaac cggcagcacg tcagacaccg catggtgata
    10801 catcgcctgt tcccgtccgc tcttggaaaa atcaccgccc atgttttcct gccagtaagc
    10861 tgccgcattt ttccggatct cctgtttcag ctcttccggc gacagcgcca gaaagtgttc
    10921 cttgtcatac tgccgaagaa tctgctccag gcaaaaatgc accatgctgc cgctgtttgc
    10981 gccggtcatc tgcattttct gccgctggta cagccggaga atgtcgttgc agtaatacaa
    11041 aaacggacac ttctgatagc gctccagcgc cgatgcggaa agctgcatgg tatcgcccag
    11101 gtaatgcccc accaatgcgg tatccgcaat ggaaaacagc ggttcgtttt cgtcgtgccg
    11161 cacggtaaac cgggtcaatc cttccaggcg ctgggcatag accgggtcat cccacagcaa
    11221 tgcgcccacg ctagcagtat ccgcagactg ctcggcataa ttctgcacat actgataata
    11281 tgccgcatga agtgtagcgc tgtaatagga agttcccagt gaggagcagg actgccgcag
    11341 tgtctcagcg ccgggaaaca tccgctgcat ctgtgccgtg accgcagagg gatagcactt
    11401 ctgctgtgtc accgtcacct gggggtaggt cagatacagc gcatgggatg cggaggacag
    11461 cagcttatac gccgccagtc ttgcatctgc catctggctc tccttggact ttgccaccgg
    11521 aagttccatt tcttccagag tctgacagtc acggtcggag aaaactgcac tgccgccggg
    11581 aaacgccggg aatgcacctt cgcagacacc cagcaggaac acgattttcg gcgcattcag
    11641 ccgggcagtg ctgccctgtg aaatcagtat ggcatccagc gtccggggcg gtgttgcacg
    11701 ctggatgctg ctggtcagag atgacagaat cgcacagaac tcgggcagtt ccagttccca
    11761 ttcctggtag agcagtgtca gatgttccag gatctctacg aaactgttcc agaccagcgc
    11821 ccattcctcc tgggtctgca tccgctgttc ttcctcagcg attgccataa gctgctgatt
    11881 cagctgttcc tcgatctcct gttctgtcag aaaatcgtac agcagccggc agaattctgc
    11941 accggtatgg aaaccacgca gcttttcccg gagggactgc aagggcgaca acagcttttt
    12001 ccggacgctt tccgcatcct cgcagctgcc gccagtaaac gctgtgttcc aggtctttcc
    12061 ctcgatttgc caggtgtagc agtaattttc cagcacagcg atctcttccg gcgtacagga
    12121 agtcatgccc gtttttccca ggcgcagcag cagttctgtg tccggctttg tgttttgcag
    12181 cagtcccagg agtgtatgca gatacaccag aaacggcgta tagcacatgg attccttttc
    12241 gtccatataa tacggcagtg catagcgttc cattgccgtt tccagaatac tcttataatc
    12301 ggacatctgg ttggacagga cggcaacatc ccggcagcgc agtgcagtat cctgcgccag
    12361 catccgccgg atcgtggcac agacatagtt tgcctcttct tccggagact gcgcctccag
    12421 aatgtgcagg tggggcgcat ctcctgtaaa gggcagcgta ttccggaaaa tgtgctggtt
    12481 cagccaccgc agttcctccg actggaatcg gtaaggcgtt tcgcagcgct ctgtttccac
    12541 ggaaatatgc agctttgccg ccagctgctt caggcgcagc attgttccct gcactgtttc
    12601 aaacagggaa aacggtgcag cagacgtgct gtcaatacgc agggcgatgt acacatcttt
    12661 gcaggaagag agcagcacat ccagcattgc atactcgtcc tcggtaaaac tttcgaattc
    12721 gtccagacag acaacatcgc ccagaaaagc atcctgtccg tttgcaatga cagcagtctc
    12781 ggtcagatcg gtctctgtat ctttcagatg atgctgggca aggagttgat cgtatttctg
    12841 gtagatttca aacagatcca gcattttttc ctggagccgt ccgttcagca gcgcactgga
    12901 agaatacagc tgttccggct ggattccatt gcggcggaac tgcgcaaaca tggcggacag
    12961 ttccgagaga aacgatgtct ggcggcattg tctgcccagg agacgcagct ttttttcccg
    13021 ttccgagacg gcggtcactg cctggtacaa cagtgccata cgagtcagtt catccgcatt
    13081 ttttctgccg tccggcacac aaccaaaacg ctgaaagaca gaacgtgcca gcgacttaaa
    13141 gctatgggtt tccagctggt tgaacgcctt cggtccgagg attcggtata gtttctggtc
    13201 aaattcataa gaaaactgct ccggaaccag cgttatcaca cgctctccgg cagccacctg
    13261 tgcctgcacc cgttccatca gacgagtgga ttttccgcag cctgcgccgc caataatcat
    13321 atgcagcata tcccctgcac ctcctgtatc acgcatcgta atgtactttc attataccag
    13381 ctaaatcccg aaaagtcaat tttacagcaa aaaagccgca atgtaacaac actgcggcaa
    13441 gtgattatcg ttattgcact gcatcaggaa ttcggctcga caatgatctc acgatgcgga
    13501 accggtgtgg agaacacgat ctgcgtcgaa gtattgccaa agcgctggat ctttccgatg
    13561 aaagtatcca gttcctgtgt agacgggaat gcgaccttaa tcagcatgga atacacgccn
    13621 nnnnnnnnnc ctgtgatgca gttgcactcc agtacgttgg gacagctgct gataaacgga
    13681 tagaactccg atttctggtc agcggaaagc tccaggttga taaaagcggt gatgtgatat
    13741 cccagcttct ggcggtcgat taccgtattg tatcccagaa tgattccctg cttttccagc
    13801 ttatcaattc gggaagaaac tgccggtgca gacaggaaaa ccttcgatgc cagcaatttc
    13861 agcggcgctc tggcattttc ctgtaacaat gtgatcaaca tggcatcaat tttatccatg
    13921 aatgtatcac gtcctttctt ttctttcgtt tcaaagactg tttcacagcc ggacaggtac
    13981 agtcttgcac cgcccatacg ccgggaaaca gattctcttt tcaagcaaat tcacagatac
    14041 agcgaataaa acaaagcaga aactaccagc cttatccatt gtcagtatca ttgcatttca
    14101 taattttagt atagcaaaaa aatatgtcga tttgatatcg gatttataaa ttttccgctg
    14161 aaacttcact ttcttatttt ttcagtccgt caatcttgtt ttttgtttgc tgaacttcct
    14221 gcaaaccatt ctggaaataa tcctgtgcct cttccagcaa atgcgccacg tacttctctg
    14281 ccgcatcctt gatatcattt gagccgttct gtgccttggt cagcatatcc aacgcacgtt
    14341 ttttcgcctc ctggaggatt gcctgttctg acagcatccg ctttgctctt gtttcggcat
    14401 cctggatgat tg
//
LOCUS       DAWENO010000041        13977 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1812_length_13977_cov_12.758009, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000041 DAWENO010000000
VERSION     DAWENO010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13977)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_13977_cov_12.758009"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3275 a         3863 c         4027 g         2812 t
ORIGIN      
        1 agctaacgct gacatcccag cacaaggcac accctacggg cgccttttca aggggaactt
       61 tcttgaagat tggtttgttt gaaaaagacc accgtagggg cgaacatcgt tcgcccgaga
      121 ctcctccatc aagcctgcgg tttgccacct cgtactgagc ggtccccttt gtcacctacg
      181 gtgacatttc cccaccccgt ggggaatcac ctctaaaaga ggaggctttg cgaaatagtc
      241 tcctctcata gaggagatgc ccggcagggc agaggagtca gatgttgaac agcctctccc
      301 cattccggca agtctgctcc agaatcgtct gcgtatccgt cccacgaacc aatgccgccg
      361 cctcggcagt ataagggatc atacggctgt cacagcgctt tccccggaac ggcaccggcg
      421 ccatatacgg gcagtccgtc tccagcacca gcttgtcctc cggcacttcc gccaaggctt
      481 tcagcgcctt tttggcattc ttaaaggtca gcacgccggt gaaaccgatg tacattccca
      541 gtttcagtac ctctctggcg gtctcggcac tgccggaaaa gcagtgtacc acgccacggg
      601 gacgcatctc ccgtaagatg ttcacatagt cctcggtgca gtcccgggcg tggatgatga
      661 ccggcagatc cagctgtttc gccagatcca gctgctcccg gaacaaccgg atctgtacat
      721 cccggtcata gccctcatag tgatagtcca gcccgatctc gcccacggca accactctgg
      781 ggtctgccgc catgcgctcc agctgcgcca gataatctgc cggctgctgc ccggcggtct
      841 ccgggtggat gcctacggcg gcatagaggt agtcgtagcg ccttgtcagt gcgatgccgg
      901 cggcactgtc ctccagcgtc gttccggcga gaacggcgtg gatgatgccc tctttcggga
      961 gcgcatccag cacctggtca cggtcctcgt caaaggcgct gtcggtgtag tggctgtggg
     1021 tgtcaaaaat ctgcgtaaac atagggactt cctttctccg gaatgcctgt gcggttgtgg
     1081 ggtttgctga aaaaatccct gccggacaag cgaaatctat tcatatctaa gacttgcaat
     1141 ttttcctgtt tcgtgctata ctaaagggta tgccggcgaa tgcagccgaa ttttcggtgc
     1201 tgctcccggt cataaaaaac gcagtttcat cataccatat ttgatagaaa aatgcaagtg
     1261 agcgtgttca gacgctgggc aggtcatttt tttcagaaag tcacccggtg tgccttgtgc
     1321 tgaaccggat atcgtaagtt aatataggct cccctaatag ggcacccgaa gggcgtgcct
     1381 tgtgctgagc tggcaagcca taggcttgac tgaggggtca atcctcctgt cagtttacgc
     1441 tgacagttca acacaagaca cacccctcag acatcctgtc aaggggaacc cataaaacac
     1501 ataccgccgt ttcagcggcg aaaaccgtat ccaaaaagga acgtgataga tcatggaaca
     1561 agaacagcaa aaacgctatc gtgcatgggc ggagatcgac ctgaacgccc tgacccataa
     1621 tgtacagacc gtgcagaaac tcctgccggc gcagacccag tatatggcgg tggtgaaggc
     1681 caacgcctat ggccacggag atgaggcgat ctgccggaaa ctctgggaac tgggtgtgcg
     1741 ctggttcgcc gtctccaatc tggaagaggc gctttccgtg cgccagttct gcccttacgg
     1801 cgagattttc attctgggct atacgccgcc ggaatttgcg ccggagctgg cacggaacaa
     1861 catcatccag ggcgtgctgt ccctggaata tgcccgtcag ctccagcact tcgcccacca
     1921 gccggtgcgc tgtcatatca agctggatac cggcatggga cgcatcgggt tccggggcga
     1981 ttctccggcg gaatgcgcca aggcgctgct gcccctgttc gacctggaaa agctgtctat
     2041 cgagggcatt tacacccatt tcgctgtggc agattctccc gaggcagacg atgtggcata
     2101 tacccaggcg caggaggaca gcatcgttgc cgtttatgac gaactggcaa agctggggca
     2161 tccgctgaag catctccact tcatgaacag cgccgccacc gtgacacgcc ccaaccccag
     2221 ggcaacgctg gcacgggtgg gcattattat gtacggctta gagccgaact atcccattca
     2281 cgttcccatg gagctgcatc cggtgatgtc gctccggagc gtggtctccc acgtcaagac
     2341 cgtggaggct ggtgcgtgca tcagctacgg cagaacatat accgctccgg gaacacgcac
     2401 catcgccaca gtcaccatcg gctatgcaga cggctatgcc cggctgctgt ccaataaggc
     2461 agaggcactg ctccacggca gacgctgccc cattgtggga cgtgtctgca tggatcagct
     2521 catgctggat gtgaccgatg tgccggaaga agtcaagccc ggcgatgtgg tcactatgtt
     2581 cggcacagac ggcggcgaga ccatcaccgc cgatgagctg gcagcactgt acgggacgat
     2641 cggctatgag atcatctgcg gcatttcaaa acgtgtgccg agagatgtga tcggggcgta
     2701 aggaacggcg atggaaaaga gagactcctc agtcaagcct gcggcttgcc agctcctcta
     2761 taagaggagc caaataaaaa gtctcctctt atagaggaga tgcccggcag ggcagaggag
     2821 tcaatttgcc cattttgaaa aggcgcacta taggggcgaa catggttcgc ccgaaactac
     2881 tcagtcaagc ctgcacagga ttttccattg caattcccga aaaaatcagg tacaatcatt
     2941 cctgtataaa aaactaaaat ttatagtagc taaaatctat agaagttaaa atctctgtct
     3001 cgtgggaatg cctgccggac aaattgtgaa agccgacgca gatgcgtcac aggaaaggag
     3061 cagctatcca tgcttgaaac acagaagatc ccctatgcac tcggaattga cgtgggttcg
     3121 accactgtca agacggtttt gctgacagaa gaaggcgaag tgctttcttc cagctatgaa
     3181 cgccattttt ccaaggtccg tgagaccgtg tgtgcaaagc tggcacagct ggcgcaggaa
     3241 tatccggata gaaagctccg ggtcagcatc accggctctg ccggactggg actggcgcag
     3301 gcggcagagc tgccctttgt ccaggaagta cacgctgcgt tcctggcggt caagcagcgc
     3361 tatccccagg cagatgtggt catcgagctg ggcggcgagg atgcgaagat catctttctc
     3421 accggcggcg tagaacagcg catgaacggc agctgtgccg gcggtacggg cgcattcatt
     3481 gaccagatgg cgaccctgct ggacgtgagc gtggatcagc tggataaaat ggcgctggcg
     3541 gcagaaaagg tctatcccat tgcctcccgg tgcggcgtgt tcgccaagtc ggacatccag
     3601 cccctgctga accagggcgc acgccgggag gacatcgccg caagcatttt ccaggcggtg
     3661 gtggaccaga cggtctccgg tctggcacag ggcagacgca tttccggaaa cgtcctgttc
     3721 ctgggcggtc cgctgtcctt tttgcaggga ctgcgcaacg cctttaccaa gacgctggaa
     3781 ctggacgaag aacacgccct gttcccggag gacggcagaa cctttgtggc atacggcagc
     3841 gccgtttccg catcgtctct gacggatacc tataccttaa atgaactgga agagatgctc
     3901 cggaacgcca agacccagac ggcactggtg gcagacaagc cgctgttcag ctatccagag
     3961 gaataccagc gcttccggga gcgccacgcc gcacacgatg tggcacgggg tgactaccgc
     4021 acctatgccg gaaaggcgta cctgggcatt gacgccggct ctaccaccac caagatcgtg
     4081 ctgctgtcgg aaaaccacga actgctgtac gagttctacg cctccaataa gggacagccg
     4141 ctggactgca tccagcggga actctctgcc ctgtatgagg ggctgagtcc ggactgcacc
     4201 atctgcggca gtgccgtcac cggctacggc gaagatctca tccgtgccgg gctgggcgtg
     4261 gacttcggca tcgtggagac tgtggcgcac ttcaaggcag cgtcgttctt ctgtccggag
     4321 gtagatttta tcatcgacat cggcggacag gatatcaagt gctttcagat caagaaccac
     4381 agcatcgaca gcatcatgct gaatgaggct tgttcctccg gctgcggctc gttcatccag
     4441 gcgttcgccg tggcgctggg ctatgacatc gccgatttct ccaggctggg tctcttcgcc
     4501 gagcatccgg tagatctggg ttcccgctgt accgtgttta tgaacagcag cgttaagcag
     4561 gcgcagaagg acggtgcaac ggtagaggat atctccgccg gtctgtccgg ttctatcgtc
     4621 aagaatgcca tctataaggt tatccgtgcg cataacccgg aggaactggg acagcacatc
     4681 gtggtacagg gcggtacgtt cctcaacgac gccgtgctgc gctgtttcga acgggagatc
     4741 ggtcacgacg tcatccgtcc ggcgatctcc ggactcatgg gcgcattcgg tgcggcgctg
     4801 tatgcccagg agaagagcag cggtaccagc accctgctga atacccagga actgaaagac
     4861 ttctcctata cggcaaaggc agccacctgc aagggatgta cggcgcactg tgcgctgagc
     4921 atcatccggt tcaataacgg cggccggttc atttccggaa accgctgtga aaagggcgca
     4981 ggcaagaagg agtcggagca gatgcccaat ctgttcgagt ggaaataccg gcacatcctg
     5041 gcgctggaac agcagaacca gccggaagag ccgaaagcca ccgtgggact gccgctggcg
     5101 ctccactact acgaccagat gcccctgtgg cacacgttct tcacggagct gggctttgcg
     5161 gtgaagttct ccgaggaaag cacccggcag acctattaca agggacagag taccatcccg
     5221 tcagatacgg tgtgctatcc ggcgaagctg acacacggtc atatcgagag cctgctggaa
     5281 cagggcgtgg acttcatctt ctacccctgc atgagctata acatcgacga gggcgattcg
     5341 gacaatcact ataactgtcc ggtggtggcg tattatccgg aactgcttat ggcgaactgc
     5401 gaaaagctga accacaacaa tttcctgtac ccgttcctcg acctgaaccg ccgcagccac
     5461 gtagtaaaga ccctgtccac catgttcagc cgctatgcca ccaaaaagca ggtggagacg
     5521 gcgctggaac acggctttgc ggcgctggag cagtaccgga aagacattgt ggcagagggc
     5581 gaagagatcg tccggcaggc gagggacgcc ggaaagcaga tcatcgtcct tgccggcaga
     5641 ccctaccaca tcgacccgga gatcaaccac ggcatccata agctgatcgg cagcctgggg
     5701 ctggcagtca tcactgagga cagcgtggcg cacctggcgc agacgccgga actccaggcg
     5761 ctgaaccagt ggacctacca ttcccggatg taccgtgccg ccaagtatgt caccacacag
     5821 ccggatatgc agctggtaca gctggtgtcc ttcggctgcg gcatcgacgc tatcaccaca
     5881 gacgaggtgc ggcgcatcct ggagaccggc ggcaagctgt atacccagct gaagatcgac
     5941 gagatcagca acctgggtgc catcaagatc cgcctgcgca gtctcatcgc cgctatggag
     6001 cagacacaga accagacaga accggagacg gtgtgaaatc ttttgtcagt gaaaattgta
     6061 gtatactgta attttcaaaa ttctatagat taggctccct tgaaaaggca aaaaactctt
     6121 ctgtcctgtc ggacaccttt tcaaagcggg caaaccgact cctctgccct gccgggcatc
     6181 tcctctaaaa gaggagacaa tatgaagcct cctcttatag aggaggtggc acgaagtgcg
     6241 gtcggagtcg cccgcagagt ttctcagcat gatacagcag cgcaaaactg catcccaccc
     6301 aaaacccaga gcattccaag gaggtaacca tacctatggc atacgcaaag tttacaccgg
     6361 aaatgaaaaa gacatatacc attcttgcac ccaatatgct ccccattcac ttccatctgc
     6421 tgatccgcat cctggaacag ttcgggtatc atgtggtgct gctggagtct acttcccgca
     6481 cggtcgtgga agagggactg aaaaacgtac ataacgacac ctgctatccg gcgctgctgg
     6541 tagtgggaca gtttatggag gcgctgaaaa gcggcaagta tgacgtagac catacggcgc
     6601 tgctcatcac ccagaccggc ggcggatgcc gtgcgtccaa ctatatccac ctgctccgga
     6661 agtgcgtggc ggaacagttc cccaacgtgc cggtgatctc gctgaacttc gccggactgg
     6721 aaaaagacag cagtctggag ctgacgccgg cgctgtgcat caagatggtg tacgccgtgc
     6781 tgtatgccga tatgctcatg accctgttca accagtgtcg tccctatgag ctgaacgagg
     6841 gcgagtccca gcaggtgctg gatgcctggc aggaaaagct gccgaaactg ttcgagagca
     6901 gcaagtatct ttccgcagag aagatctatg cccagattct gaaggacttc gccgccatcc
     6961 cacggagcaa aaagcccaag atcaaggtag gcattatcgg cgagatctac gtaaaatatt
     7021 cgccgctggc gaacaaccac ctggaggact tcctcatctc cgagggctgc gagcctgtcg
     7081 tgccgactct gctggaattt gtcctgtact gtgcggcaaa tacggagacc aacagccacc
     7141 tgtatgacta caagaacttc aagaccctgg cgttcgggct gggctataag ttcctgcaca
     7201 gcaagcaggt gcagatgatc aaggcgatcc agaagcacgg cgagttcacg ccgccgcacg
     7261 atttcgagga tctgcgccgg gctgcggata agtatatcag ccagggcgtt tctatgggcg
     7321 agggctggat gattaccgcc gaaatggcag tgctggcgga gaccggcacg gagaatatca
     7381 tctgcaccca gccgttcggc tgtctgccca accacatcgt ggcaaaaggc atggcgagaa
     7441 ccatcaagaa cgcctatccc aacgccaaca ttgtggcgat cgactatgac cccggcgcaa
     7501 cacgggtcaa ccaggaaaac cgcatcaagc tgatgctggc gaacgcccgg atggagcagg
     7561 agaacaaggt ctgatggtga agtccatttg ttttaccgga caccggcaca tcgccaaaga
     7621 aaagctgccg gcggcgctgg cggagctgcg gcgtgtgctg gaaacggcga tccctcaggg
     7681 gttcgtggat ttttacgccg gcggtgcgct gggatgggat acctactgcg cacagaccgt
     7741 gctggatctc cgggaagagt atccctgcat cgccctgcat ctggtgctgc cctgttcccg
     7801 tgcggagcag acggcacgct ggacagaggc gcagaaagcc gcctatgact gcatctaccg
     7861 ggaggcggat tcctgtgagt tcgtctccgt ggactatacc aaggattgta tgcgcctgcg
     7921 gaacaagcgc ctggtggagc tggcggactg ctgcgtctgt ttctgtggcg agccggaggg
     7981 caggagcggc acggcgcaga ccgtccgcat ggcacggcaa aagggcatcc cggttatcaa
     8041 tctggcatcg tgaaatacaa aagccacgtc ggcgtgacgt ggcttttttt gcgtatggaa
     8101 agggatgctc agttccgggc agcagccttt tcatttcgcc ggaacagcag acggaacagc
     8161 cagcgcacca gcggtccgat gtagaacacc tgtaagatca gtgccgccgg aaaacactgc
     8221 ccggcaagct ggatccagcg tgggaaaaag cccgggttta cgccgccgtg gagcagggac
     8281 gcaaacagcg tcaccgcctg gacgaccatg gagaccgtca gcccctgcac caccagggtg
     8341 aacaggatct ggggcagttc ccgtgggtgc agcagtttca gtgcgatgcg tggcgcaatg
     8401 cggctgacgc cgaaaaagac cagcaggaac acaaaggcga attcgatcca catttcccgg
     8461 atggactggg cgaacagacc ataggtcacg ccgcctgccc ggagcacccc ggtatagcag
     8521 gtcatggaaa agaccatgca gaataccatc aaggcggtaa agaccacgct ttccagtttt
     8581 gtttttggca tatgcagtta tcctcctttt ccgcaaaaaa aagaaacgac attaaaatat
     8641 tccgagacca tcagacatgg gtctcagaga tatttaatgt cgtttctttt atgcggttat
     8701 tctgtaatta taataccacg ttttttcaaa tttgtcaaga gtgaatcaaa ctaaaatcac
     8761 gcaaatcatt tttgcaactg atgcactttg tgttgaatta gcaagtcata ggtttggctg
     8821 aagaacgaat ccccctgtca gcttacgctg acatccccct tttcaagggg gattccccac
     8881 agagtgggga aatgtcaccg taggtgacaa aggggaccgc tcagtacgag gtggcacgga
     8941 gtgccggagg gattatccct tttatttgtt tgttctaata tctcagcaca aggtgtacct
     9001 tacgttatcg tttcaaaggc agttttctaa aaaattggtc catggctttt tcaagaaatc
     9061 ccttgcattt ccggcggaaa tatgctatac tgaaaacaga ctgacggcac aggcggcgat
     9121 gcctgcgccg gaaacaacca caggaggata ctatggcaaa gaaaaatcag aaaatggtgg
     9181 aagagatcac ggcaatggac gtggatttcg cacagtggta caccgatatc tgcaaaaagg
     9241 cagagcttgt ctcttatacc agtgtaaagg gctgtatggt cattcgtccc tacggctatg
     9301 ccatctggga aaatatacag cgcatcctgg acgcaaagtt caaggaaacc ggtcacgtga
     9361 acgtcaatat gcccatgttc atcccggaga gcctgctcca gaaggaaaag gatcacgtag
     9421 agggctttgc gccggaagtg gcatgggtca cctacggcgg cagtgaaaag ctggaagagc
     9481 gcctgtgcgt tcgtccgact tcagagactc tgttctgcga tcactacaag gacatcgtac
     9541 acagctatcg tgacctgccg aagctgtaca accagtgggt cagcgtagtg cgctgggaaa
     9601 agaccacccg tccgttcctg cgctcccggg aattcctgtg gcaggaagga cacaccatcc
     9661 acgctaccgc agaagaggcg atccaggaga ccgagcgtat gctgaacgta tacgctgact
     9721 tttgcgaaaa gtgcctggca atgccggtgg tcaagggcag aaagaccgag agcgacaagt
     9781 tcgccggcgc tgtctccacc tatgccatcg aggcactgat gcacgacggc aaggcgctcc
     9841 aggctggtac atcccactac tttggggacg gctttgccaa ggcgttcggc atccagtttg
     9901 ccggcaagga caacaagctg gcatatccgc accagaccag ctggggcatg accacccgtc
     9961 tgatcggcgc tatcatcatg acacacggcg atgacagcgg tctggttctg ccgccggcag
    10021 tggctcccgt acaggctgtg atcgtcccca tcgcacagca caaggaaggc gttctggaca
    10081 aggcgaacga gctgtaccag gcactggctg ccaagggcat ccgggtcaag ctggacgact
    10141 ccgacaactc tccgggatgg aagtttgcgc agtacgagat gaagggcatt ccggttcgta
    10201 tcgagatcgg cccgaaggat atcgagtccg gacagtgcgt ggttgctgct cgctttaacg
    10261 gcgaaaagca gaccgttgcc ctggacgaga actatgcggc gctgtgtgac accgtgaccg
    10321 acctgctgga gaacgtgatc ccgcagggaa tgttccagaa ggcgctggaa aaccggaaga
    10381 acaagaccta tgtctgccag accatggacg agatcaccca ggcgctggaa gaaaagggcg
    10441 acggctttat cgaggcaatg tggtgcggcg acgaggcgtg cgaggacaag gtgaaggaag
    10501 tcaccggcgt tggctcccgc tgcattccgc tgaaccagca gcacatttcc gacaagtgcg
    10561 tctgctgcgg caagccggca aagcacatgg tgctgtgggg caaggcatat tgatcttacc
    10621 gcaaacagaa gaataacagc agccgtccgg ttcgccgggc ggttgttttt tgttgttgga
    10681 taaaattgtt ccagaatcac cgtaggggcg aacatcgttc gcccgagact cctctgactc
    10741 ctccggcact gcgtgccacc tcctctaaaa gaggagactt tgcgaaattg tctcctctta
    10801 tagaggagat gcccggcagg gcagaggagt tgatttgcct cccttgaaaa gggaggtgga
    10861 tgctccgtga ggagcattcg gagggataat ccccctgtca gctaacgctg acatccccct
    10921 tttcaagggg gacattctta gaaattagtt tgtgtgcttg aaaaatatta ccgtaggggc
    10981 gaacatcgtt cgcctgagac tcctcagtca agcctgcggc ttgccagctc gtactgagcg
    11041 gtcccctttg tcatctacga tgacatttcc ccaccccgtg gggaatcacc tctgtagagg
    11101 agccttatat cgagttctcg aaacaaaaat cccccggagg ggtcataggc agcttgtctg
    11161 ccgtgacgtt cccctttctt gaacgctaaa ttggggcgaa aggtcagcgg tggcttgcta
    11221 tccttgggca agtctgctgt cgccatttcc gctgggcgac ggactgaccc gccgtccgtt
    11281 gtaaattggc tattgcaatt tttgttgttt tcttgtataa taaaagatac agcataccgg
    11341 gaaggcatag gctttcgggg gaacgggtgc tgttttcgca ttcgttttgt tccggcagaa
    11401 aaccgcccgg aacactcggg aaaggaatct ctatgaaaca tatgaccaaa aagatcgccg
    11461 gactgagcct cggtttggcg ctggtgctgg gctgcggcgt gggtacgctg tcggctgcgg
    11521 caacctcggt gggcgatgtg atcgctcatg cctacgctgt gggtctgccg gaggcgcaga
    11581 tccagcagtg catcgctcag tacagcggcg gcaattatac ctccgaacag tgcgataggg
    11641 caatcgctgc cctggataac tgggcaagcc agcgtgacca gaagatcgag gacgaactga
    11701 acaaaggcac tactgccccg gcagataacg ccgactcctc cgccggcgag acgaccacca
    11761 ccgccgcaaa ggcaaacggc agcagcgcag cgcagacgac cacaccctct gccaaggatt
    11821 ttatggatat gaccctggat gaaaaggtca gctatatcaa tagcctgccg gcagataaac
    11881 gggacagctt cgtgcagaac atgagcaacg acgagcgcaa cagctttctg aaacagctgg
    11941 acaccagcga ccagctggat atcatcgcca gcatggcaga cgtgggcgag gcgttcggcg
    12001 tgaacttctc ggtggatgag atctccgacg gcgcaatttc catcagcgcc catgacgcag
    12061 atggcaacct ggtgggcgtg tccacctttg gcaatacggt agaggcgacc ggtatcccgt
    12121 ataccgttcc ggtgctgatc ggcggtggcg cagttgtcct ggcggcagcc ggactgggca
    12181 tcgtcctctg ccgcagcaag aaatcgtgag gcggcgtgta tgaaaaagaa aactacatcc
    12241 agactgccgc tgcccctgtt ggcgcagatc ctgctgccgc tgctggtggc tgtgctttgc
    12301 gccggcatcc tgctgctggg cagcattcgc ccgtatgaga tggcgaaaac ttatctgaaa
    12361 gttgccttta tggacggcag cggcgttgtt tctgacaacg gcacaaacgg gctgaacatc
    12421 gtccagacgg acatcgacac cgactatacc ggcgagaccg cagaagaggg tacgccggtg
    12481 gtctcggact atggcagcca gtgtgctgtg ctggaggcga aatccatcgg actgtatgcg
    12541 ccggtctact ggggcggcgg tgcggaactg ctggaagagg gcgcctgcca gacaccggca
    12601 tctgcactgc tgggcgcatc gggaaactct gtcatcagcg cccatgtcaa cacctttttc
    12661 catgatctga accagctgaa ggtgggggat acggtgacgg cgtataccac ctacggcaaa
    12721 ttcacctatg aggtcacgga gcagatcacc tttgacgcat cggacaagtc ttatttgaaa
    12781 aagacggacg acgagcgtct gaccctgtac acctgcgaga tgcagctcct cggctcatcc
    12841 tcgaaacggg tgggcgtggt ctgcaagctg acggataagg cgttctatac cgatgccggc
    12901 acaagtgcgg aaggaggcaa ccaatgagca aagcaacaac ttccggaatg ggaaaaggcg
    12961 gactgctgct ccggctgatc ctgtcgattc tgctggtgtt ttccctgctg ggaacagtgg
    13021 gcagcgccgt gggcgtttcc gtcctgtgcg gtccgtccca gctgatctcc cagatgcacc
    13081 gacacgatgc tggtcagaag gtctacgaca gcctgaatac caggttccag aacgactata
    13141 acaccaccgc tgtcccggca gaggtataca tggacaccat ctccgtggac tggctggaac
    13201 agtgcatgga gaacaaggtg acggcgctct atggaaaagg cagcggagac attgattttt
    13261 cggcactgga gagcagcatc acagactact ttgaaaagta cgcagaggag aacaactgcg
    13321 caaaggatga tacctacaac gaaaagctcc gggagaccat cgacaacgga gaaaaggtca
    13381 tctccgatgc cacagacctg ctgcggacag agaccctcca gaaatcgggc tatctgtcca
    13441 agctgcataa gctgcggaca ctgacctttg caggcgaggg tgtctgcggc gtgctgacgg
    13501 tactgctgct gttgctgctg cggaaccgct actggatcgg caccggctgt ttcggcgcag
    13561 gcgcattgct gactgccggg gcaggggtga tcttcggcac aggcgtgatc catcggttcg
    13621 tactgaaaga ggcggctgtt tacgccgtcg tcaccggcac gatgacggca ctcaccacgg
    13681 cggtgctggt ctccggcatc gtcctgctgg cgctgggcgt ggtgctgctg gcactgcatc
    13741 tgaaaggaca gcgctcggag aaagcggaat aaaggcaaca ccgcacaaag cttgtgcgta
    13801 agatgcgaag ccggattttt cggcagattg catagaaatt tcgcaggcat actttgtagt
    13861 cgcaatcaat cctagtgcga cgctcctttg tcctcgggca gaatcctgcc tcgaacgcag
    13921 tcgtctggca aggaatttct gggcaatatg acggaaaatc agcaagcaga ttgcgtg
//
LOCUS       DAWENO010000042        13885 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1826_length_13885_cov_8.130224, whole genome shotgun sequence.
ACCESSION   DAWENO010000042 DAWENO010000000
VERSION     DAWENO010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1826_length_13885_cov_8.130224"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2610..2629
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7485..7514
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8630..8639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4159 a         3072 c         2890 g         3704 t
ORIGIN      
        1 gatcatacca ttcttggaaa acccgggacg ccggaacggg cagaagaaat gatgcggcgg
       61 ttatccggga aaacgcacac agtaattact ggagtggcgc ttgccaaagg aaaagaaatg
      121 ctttcgttcc aaaccagtac agatgtgaca ttttatcctt tgacagaaaa gcaaatacag
      181 gactatatcg ctacaaatga accaatggat aaagcaggcg gctatggcat acaaggctat
      241 ggcgcactcc tggtgcaaaa aatctgcggt gatttctaca gtgttgtcgg acttcctatt
      301 gcagaaaccg ctcgggcatt ggaaaaattc caaaaacaat aaatccagct gcgaacagaa
      361 ataacagaaa gcagaaaaaa tcggaaagga acaaccatga acaaaaaaga actctctgag
      421 atacgaaaaa acttttctga aaccagtgat cttttcgtat tgaatcaagt ggcaactgca
      481 tttgtagaca gcgaaaaaaa cattcgctgt caaacaagcc gagcctatca caatatccct
      541 tctgaagaaa gcgactgcct catggccaca ctgaagcgtg tcctcgccgg aacgctggga
      601 aaggggcttt tggagtatga atttccaaaa gaggcatatg aggaaggcgg cagtcagtcc
      661 attctctatg aggcactgaa caccaaactg gaagaagaaa atgcagtgac aaatttgatg
      721 gaacagattg ttcacaacat ggactatgtt tctacctatg cgattctgat cggacactgc
      781 acctatactg tttttcagaa atcaaaaagc gatgagatcg acccctatca aagtcacgat
      841 taccgctttt tagttgctgc gatttgtccg gtggaagttc gtgtagacgg tctgatctat
      901 aacgaggacg acaatgccat cgaaaagaaa aatgtctacg accgcattgt tgccgagatc
      961 cccacagacg gatttttata ccctacattt acaggcagag gatcagatgt caaccacgtc
     1021 ctgtactctg cacacaagcc aaaagatgtc aacatcagca ttatcgaaaa tgtcctgggc
     1081 tgtccgttta accgcacggc acaggaacaa aaagaaactt ttcaaaacat gatgcaggaa
     1141 attgtttccg atgagctgag ctatacagtc attaccggcg taaacgaaaa gctgcgtgac
     1201 attgctgcgg aatatgcaaa tgagccagat ctgcccgttg tggatgatat tcacgtacgg
     1261 gatattctgc tggattccgg cgtcagccag gaacgtgcag aagagatgca gatggcatat
     1321 cgtgaggcaa cgcagaacaa gcccattatg gtcaataacc tgattgacag caaaacaacg
     1381 atcagtctgg acggcatcac agtatccatc ggaaaagatg ccacagataa ggtacggaca
     1441 caatgtgtta acggcagacg ctatctgctg atcgacctgg atgatccggc ggtggaaatt
     1501 aacgggttaa ccgcacgaat tgcagaacag ttaaatgcgc cggaaacaga agaatctaca
     1561 gatgatgacg agtaaactct gaggataatc ctatgacatt acagcaatta aaatacgccg
     1621 tggtaactgc tgaaaaaggt acggtaagtg aagcagctgc tgcgctttat attgcgcagc
     1681 caagcttgac gaaagcaatc aaagaactgg aaaaggaaat gggaatcact atttttcacc
     1741 gtacaaacaa gggtgtatcc gtttcaaaag aaggcgaagt ttttctcgga tacgcacgtc
     1801 aggtattaga acaggcatca ctgattaccg aaaaatatgt tcaccaatcc ggcgggaaac
     1861 aagaattttg cgtatccaca cagcactatt cttttgcagt aaatgctttt gtagatctca
     1921 tcaaagccta cggcagcgaa aattatgact tcagcctccg ggaaacacaa acctatgaaa
     1981 ttattgacga tgttgcaagg ttaaaaagcg aaatcggtgt cttatatctc aacgagttta
     2041 atcagactgt tctgcacaaa atcctaaaag caaacgattt acagtttact gagcttttcg
     2101 aggcggaacc gcatattttt atcagcaaca agaatcctct tgcagaaaaa actgctgtga
     2161 caatgcagga tcttcagcca tacccgtatt tgtcatttga acaaggggaa cacaattcct
     2221 tttactattc ggaagagatt ttcagcacag aaatacgttc taagaacatt cgagttcgtg
     2281 atcgtgctac gctgttcaac ttactaatcg ggttaaatgg ttacaccatt tgcagcggtg
     2341 tgattgacga agaactaaac gggaaaaata ttgttgccgt tccgctccgt gaaaagggga
     2401 gtatgcacat cggttacatt accagaaagc agtctatccc tagcagattg ggaagtatct
     2461 atcttgacgc actgaaacat tacgctaaaa acacataatt attgctgctg ataaagcggt
     2521 tagatatagt ataaagctat atctaaccgc tatttttata tattttacat agcagaaatt
     2581 atcggctata atagaactac aatcagaaan nnnnnnnnnn nnnnnnnnnt tccactagaa
     2641 tggagttcaa atatgaaaac aacgattatc ggatttccca gagtcggcag cttacgggaa
     2701 ttgaaatttg caagtgaaaa atattttcgt aaggaaatca cagcacagga attgcaggac
     2761 accgcaaaaa cacttcggaa tacacactgg aatctgcaaa agacaagcgg catcacatgg
     2821 atcccgacaa acgatttttc gttttatgac aatatgctgg ataccgcagt tttgctgaat
     2881 gcagttccgg agcgttaccg tgcgctggcg ctttctgaac tggacacata ctttgcaatg
     2941 gctcgtggat accagggcgc agaaggtgac gtaaaggcac tggctatgaa aaagtggttc
     3001 aacaccaact atcactatat ggttccggaa attgatgata ccacaaagat ctgcctttcg
     3061 ggagaaaagc cgtttaccga ataccaggag gcaaaggcag caggcgtaaa tgcaaaaccg
     3121 gttctgatcg gtgcatttac atttctgaaa cttgcacgtt ttaccggaaa aaaatctgca
     3181 tccgactatg ttgaatctac agcacaggca tattgtaact atctgaaaaa gctgtctgaa
     3241 ctcggtgctg agtgggcaga atttgacgaa ccgtacctgg taaaagatct ttccgcagag
     3301 gatattgcac tctttacagg actctatcag aagattctga gccaaaaggg aaatgtcaag
     3361 gttctgctcc aaacatactt cggagatgtt cgtgattgct accaggaaat tgtaagctta
     3421 gatctggacg gcattggtct ggacttcgta gaaggaaagc agacactcca gcttgtacag
     3481 caaaatggat tcccaaaaga taaggttttg tttgccggcg ttgtaaacgg caaaaacatc
     3541 tggcgcaatc actatgaaaa gacaacaaat cttttacagg aactgacaaa atacagcagt
     3601 cagatcgtgt taaatacttc ttgttcctta ctccatgtgc cttatacact gcgtcacgaa
     3661 acaaagctga atgaaaatta taaaaagcat ttctctttcg ctgaggaaaa gctgaacgaa
     3721 ctggctgaac tggcgaatat tattgacagt gcatctccga aggaaacacc tgcgtatcag
     3781 aaaaacaagg cacttttcac cgaagagaga ttctgtgaaa ataagggcgt tcaagaaaag
     3841 gttgcagcac tgacagaaaa agattttgta cgtctgccgg aattctcaga acgtgaaaag
     3901 attcagcaca aggttctgaa cctgccgctt ttgccaacta caacaatcgg ttcgttcccg
     3961 caaacaaaag aggttcgtgc taatcgtgca gcttttcgaa agggcgagat cacagaggaa
     4021 cagtatgttg ctttcaacaa gaaaaagatt gcagactgcg ttgcattaca ggaagaaatc
     4081 ggtctggatg tattggttca cggcgaatac gagagaaacg acatggtcga atattttgga
     4141 gagtgtctgg acggctacct gtttacagaa aaggcatggg tacagtctta tggtactcgc
     4201 tgcgtaaaac cgccgatcat ctggggtgac atttgccgtg caaagcctat gacgttaaaa
     4261 tggtccgttt atgcgcaaag tctgacgaaa aagcctatga agggtatgct gacaggtccg
     4321 gttacaatcc tcaactggtc tttcccaaga gaagatattt ctctgcagga atgtgcttat
     4381 cagatcggtc ttgccattcg ggaagaagtt ctggatctgg agaaaaacgg cattcaggtt
     4441 attcagatag acgaggctgc acttcgtgaa aaactgccgc tgcggaaatc tgactggaaa
     4501 tctgattatc tggattgggc aattccggca tttcgtctgg tacacagcgg cgtaaaaccg
     4561 gaaacacaga ttcacactca tatgtgttac agcgaattcg cagatatcat ccaggatatt
     4621 gacaacatgg atgcagatgt aatcacattt gaggcaagca gatccgatct tacaattctg
     4681 gacacactga aagaaaataa cttccgcaca gaagttggtc cgggtgtata tgatattcac
     4741 tctccgagaa ttccgactac agaagaaatc aaatctgcaa ttgaaaaaat gctgtctcgc
     4801 atttcaaagg aaaaactgtg gatcaatccg gactgtggat tgaaaacaag aggcatccag
     4861 gaaaccattc ccagcttggt acatttggta caggctacaa aagaacttcg ataacacgct
     4921 ctagctaaaa tccattcata aaataaaaac atcacaggga atttcttttc tctgcgatgt
     4981 ttttatttat tctgatactt ctgacaacgc cagccgcaaa cgtaccaagt caaatccatt
     5041 gaccacttga tcctgtgtca tatcagcacg taaagcctgt tcctgtatca attccaaatc
     5101 gcccagcaaa tacttttgca gcataaccaa atctgctgca ctgcatattc cgtcttgatt
     5161 caagtcgcca ggcacaacag cagatacaac atttttgatt tcacaaacgt ccaccgtttt
     5221 gcttgtgccg ccctgagaaa gaatcctcac agaatacacg ccacaatctt cctggtcttt
     5281 cgctgtaacg gtatatgttt tctccacagc acctggtatt tcttcgccat tatgcagcca
     5341 ctgatatgtc aaatccgttc cggaggtttc cggttctaaa gtaaccgcat cattttccat
     5401 aagagaaatc gtctgttttg ctgaatacac atcaaaatta tcttgaatgg tatagctttg
     5461 tactttgata gaacctgcat tcacccagga attcgactga tcttgaactg ttacactgat
     5521 atctgcgatc tccacacctg gctcaaaccc agtcaaatcg ccgagcggca gacggtattc
     5581 aatgatcctg ccattgtgtt tctgagtaca agtgcttgga acgccttgat taaagtacac
     5641 aaatccattg ccctgatagt agatccagta gctctttccg tttgccttgt tcttcaacga
     5701 caaattgtat accggagaaa ccgcatcgta agtcagatct gcaaaaacat aaaggtactg
     5761 gtcattattg gtgatataca agcgacttcc attttcatcc gaggcacaca gattgtcctc
     5821 tgtgtattcg ctgtcattgc tgacagaacc atccacgaca gaaatatctt gatacgtgta
     5881 catttttcca tctgaatgcg agacatcctg ctcagcaatt gtctgataaa aactgttgtc
     5941 tgtttgtata gtgtctgctt ttgtcacaga aaagctgccc agataagttg cgccgatcgc
     6001 agaattccag acagcatggt ttgcgaactg cacagaacgc acattcattt ctccaaggct
     6061 gttttctccg gaagaaatct ttaggtaaac attccacttt ccaggttcca actcaccggg
     6121 aagtttcaga tcaaattctt tttcgtttgt ggaacaggaa taccactgat tcgtatttgc
     6181 atccacttct gttttgatgt aatttccgtc cttctccaga atgatctctg cattttgccg
     6241 catgatcgga tttgcaaatc ccgtgttttc aatttttgtg caaactctca gcacgtcgtt
     6301 ttgtgaaacg gattcactca aatcacaatc ccgaagtaca aaacgatagc caagatgatc
     6361 tcgaataaac ttaaaaacag tttcgccgta atatgcgctg ttgtccacac cgtctacatc
     6421 ataggatttg ccaaaagtgt aatctttata aagcgcatat atgttggaat tgatataact
     6481 caaatgagaa tagtacatct ccggtactgc attttccgga agatatgtgt catatttttt
     6541 tgcaaagtcc aggtttccgg aaaattcgcc gccgtaataa gatgtcaagg tttgctggcg
     6601 cagccatttt aagtcacgtt tacggtcgtg gaatgttccc aagtccgaat cagaccccat
     6661 gtatccatcg ttgtacaagc ctacacgaga cgctttgctg ccgggttcca acacatattc
     6721 gcccagttct gactcttcaa tgccagccca ttttgcaaag atattgggtg ttctcactgt
     6781 aaccggaatc ggatccggaa caacatccag cagcaagtcc aggagttttg ccttatcttc
     6841 cagagagcag tattttccac cccattgttc gccataacag ccaacaaatc cgctttctac
     6901 ataggcaata atgtcttgat agtcctctaa aaagccatcc tcacgaatct gttcaatgtg
     6961 cctgcacatc tgttcaaatg tagcaggttc cggatttcga acgccattcg catcgtatcg
     7021 aaagcgaatg ccaatggtgc atccgttttt tcggcagttt tccagcgtct ttcgaaaatt
     7081 cgtgaaaaag gtttcgtcca ggtcataatc cgttccatcc acatatgttc catcttcatt
     7141 ggtcgtaccg tttgcaccgc tggaaaaggc accaatatct acaaataaaa caaccagact
     7201 cgatgtggga ttatagactt tcgtatcatt cggtttgcag ttgacagcta cagtcgaact
     7261 atatccagca ccaggattga gaagtgtttc cgtgctttct gtataatcta ttccggaatc
     7321 gaccagagga tcttccgacg cataactcga caacgccggc agccatgtga taccgcaaac
     7381 aaataccgct gctgccgcaa ttgcttttgt tagtttcata tattttttct ccttttcaat
     7441 tgattgattt gtatatattt attataacac agacgaactt tttgnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnaagcat aaatttcaaa agagatagga aagaatatcc aattgcacta
     7561 ttttgaacta tctatccgcc gatcttccat cctctatttt tcaatagatt tcctttacaa
     7621 atcagattac tcatgctata ataaaataca gaaaacaaca taaatcacaa aaggggagaa
     7681 aggcataatg gaatacttcg aaaacacgac acgcatcacc aaagacggct atgctgcaat
     7741 tttgcaaatg aagagaaaac agcatcacat ggtatgccga gcgttaggaa ttcttctggc
     7801 gattcctttt ttagaacgtt tgatatacaa ctttattttg ctatgcctca aagaccgttc
     7861 tgactttcac tttggttttc tggatattct ttttatcatt ctgctgtttc tgggcatatg
     7921 gttctggaac ctgccaaaaa agcagattcg ggattatatt tctcgcacca aagacaaaat
     7981 tgacttagag gcagtcaacc aatacacatt cttgccggaa tatattcgca tgatgaccac
     8041 atcctccatg gaaaaatatc agctgggata tgaaaaccta acctggatcc gaagtgacaa
     8101 gcgatggatc gtactctatt tctccaacca ggacttcacc atgctgatag acaagcaggg
     8161 ttttacacac ggaaacgcca acgcctgcct cagctttttg aagaacaaac aggatggaaa
     8221 gtaaatcgaa aagcataaag agtgtatttg ttcacaaaat attgtagaat atttttatgc
     8281 aaaatggtac acttatcata gggaatagac gaaatgccat gtgcagaaat taaaatgaaa
     8341 taggaggact tgtttatgaa acgcagaatc ggaatcttga ccagcggcgg agactgtccc
     8401 ggactgaacg ccaccattcg gggcgtcgcc aaagcctgct acgaaatgct tggaacagaa
     8461 aatgtggaaa tcgtaggtat ttcagacgga tattacggct tgatccataa tatctgtaag
     8521 gaaatgtctc cggacgattt ttccggtatt ctgacccgtg gcggaacgat cctgggaaca
     8581 aaacgtcagc cgttcaaaat gatgaaagtg atcggcgacg acaatgtggn nnnnnnnnnt
     8641 ggacaaggtc aagaacatga aaaagaccta ccaggcacaa aagctggatt gccttttgac
     8701 attgggcgga aacggcacac acaagacctc aaaactgctg gcagacgaag gcttgaatgt
     8761 cataggcttg ccaaagacga tcgacaatga tatttatgga acagatgtca cattcggttt
     8821 ccatacagct gttgacattg ccacagatgc actggatcgg ctgcacacaa ctgcaaacag
     8881 ccactcccgg attctgctct gcgaaatcat gggaaacaaa gccggttggc tcacgctgaa
     8941 tgcaggcatt gccggcggtg cagatattat tctgattccg gaaattcctt acgatattga
     9001 tgaagtttgc aagtctgttc tgaaacggag tgaaagcgga aagaatttct ccattattgc
     9061 cgtagctgaa ggatgcttcg ataaggaaga ggctaagtta aaaaagaaag agagaaccag
     9121 actgcggcag gaggcaggca tcacaactgc caccaatcgt attgcagctc agattcagaa
     9181 aaacacaggt ctggaggcac gtgtctgcgt accggggcat atgctgcgtg gcggcagtcc
     9241 cagcgcatac gaccgcattc tggcaacaca gttcggcgta tacgcagcag aattgattcg
     9301 ggaagagcat tacggaatgt ctgttgcaaa ggtaggcggc gcaattactg caaacaagct
     9361 ggatgacatc gccggaaaga caaaatttgt ttcgccggat gaccagttag tacagaccgc
     9421 aagaaatctt ggaatttctt tcggtgacta aattgtactt gatcgtatat atgacaaaaa
     9481 gcggatgcac ttcttttcgt gcatccgctt ttttacagac cgcatcaagc ggttctgtat
     9541 atataaatcc ttattacttt atcatgtttc agaaaccaca aatctgccat tctgcttgcg
     9601 caaaatacgc tgattcattg agccacgaaa tgccagctgc aaagactttt cctcttctat
     9661 gaaaggacca tccaccaata catcaatctc tgaaagaaga gcatccgtca catcacagcg
     9721 gtagggactt tctgacagca gttccgattc cagaacgcat ccagtataac accagatgtc
     9781 tttctgtgtt ccaaatgtgt ggcgaaccgc ctgtaccaat ttcagcaaaa ctgcttgatt
     9841 ttccggttcc atcggttcgc cgcctatcag tgtcaaacct ttcacatata ccggtttcag
     9901 cagttctaaa atgtgtgctg tggtttcctc ggtataggga gtcccatact cgaaacacca
     9961 tgtctccgga ttaaagcaat tcttacaggc gtgtctgcat ccggaaacaa acaaagagac
    10021 tcgcacacct tctccattgg caatatcgca aggctttatt gctccataat acatttttgc
    10081 atccacctct tttacaggtg cataacacga tcacggattt cctgggtacg gccttgattc
    10141 cagaactgcg ttccgatgta accacaggtt ctgcgtgcga cattcagttt atcctggtca
    10201 cggttgccgc agttcgggca ttcccatacc agtttaccat cgtcctctac gatctggatt
    10261 tcaccgtcat aaccgcagac ctggcaataa tcgctctttg tgttcagttc agcatacata
    10321 atgttttcat aaatatgctg cattacagca attaccgccg gaatgttatc ctgcatatta
    10381 ggaacttcca catagctgat cgcaccgcca ggggacaact cctggaactg tgcctcaaag
    10441 gaaagcttat caaatgcgtt gatcggttca gttacgtgaa catgatagct atttgtaata
    10501 tagttcttat ctgtaacgcc ctcaatcacg ccaaaccgtt cctgcaggca acgtgcaaac
    10561 ttgtatgtag tggactccaa aggcgtgcca tacagactga aatcaatgtt ggtttccttc
    10621 gcccacttct ggcaagtttc gttcagatgc tgcataacac gcagtgcaaa cggtgtcgca
    10681 gaaggatctg tgtgcgactt accggtcatg tagcgtgtgc attcacacaa gccagcatat
    10741 cccagtgaaa gagtagaata accgccgtag agaagcttat caatcgtttc gcctttcttt
    10801 aagcgagcca aagcaccata ctgccagagg atcggtgcga catcagacgg cgttcctttc
    10861 agacgattat gacgacacat taaagcacgg tagcacaact gcaaacgctc atcaaagatt
    10921 tcccaaaagc gtttttcatc tctgccggaa gagcaagcaa tatccaccag attcaaagtg
    10981 acaacgccct ggttgaatct gccgtagaac ttgtacttgt cattctcatc cttataaacg
    11041 gtcaggaaag aacgacatcc catgcaggta tagcaattac cttccttcaa agacttcatg
    11101 atcttttcgg aaatataatc cggaaccata cgctttgcgg tgcattctgc tgccagcttt
    11161 gtcaaataaa aatattttga gccttcccgt atgttatcct cttccaacac atagatcagt
    11221 ttcgggaatg ccggtgtgat ataaacgccc ttttcatttt tgacaccctg gattctctgt
    11281 cgcagcattt cttcaataat cagcgccaga tcatcccgtg tctgaccttc cggtacttca
    11341 ttcagataca taaagacagt tacaaacgga gcctgtccat tggttgtcat cagtgtgatt
    11401 acctgatact ggatcatctg tacgccttga atgatttcat tgcgtacccg catttccgca
    11461 atctgattga tggtttcctc atctgtttcc tttccaaggc gttcaaattc ccggcgaaca
    11521 tcccggcgaa acttttcacg actggtttgc acaaaaggtg caagatgaga aagggtaatg
    11581 ctctgaccgc cgtattgtga acttgcaatt tgtgcaatga tctgggtgga gatattgcaa
    11641 gcagtggaga agctatgcgg cttttcaatc attgtttcac tgataactgt accattctgc
    11701 agcatatctt caaggttcac cagacagcaa ttgtgcatat gttgtgcaaa atagtccgca
    11761 tcatgaaaat gaatgatacc ttcccggtgt gcatctgtaa tgtcatgggg caggagaaaa
    11821 cgagttgtaa tatctttgct gacttcacca gccatataat cacgctgtac gctatttaca
    11881 accgggtttt tattagaatt ttcctgtttt acttcttcat tttcaaaatt caaaaggctt
    11941 aaaatcttct ggtctgttgt gtttgactga cgcagcaaac ctcgttcata tcggtaggtg
    12001 atataatttt tcgcaactgt aaatgcgccg tacttcatga tttcaatttc tacccaatcc
    12061 tgaattgttt cgacatccac agcacgattc atatctgcac agcgtgcctc aatatcaccg
    12121 gcgatcttct cgatctgctc atctgaaagc tgttctgcct caataacggt aatattggcc
    12181 tttcccacag cagctatgat cttggcacga tcaaaaacgt cctccttgcc gcttcgcttg
    12241 ataattttca aaattctctc ctccgattct aatgtcacaa ggtttttcag taaatttcaa
    12301 aacaatcatt gtcagagaca atctgcaatt tgcttaaaga tttacataaa aaatcatcct
    12361 gtcttatgtg cagttgcaca aacacaggaa atacgaaaat catacagcat acatttgaca
    12421 gaaaaagcac tttcataaga caaacagaaa gctgcatttt cagcagcttt tatcatttca
    12481 gttatgaaag cgtcagtcgc aatgtactgc gttttgcagt atttattata ctgcttttcc
    12541 gtttttctgt caagcaaatt ttttatgtat tttccgtcca aaacacaaca aatagtatct
    12601 ggagtaaact tcaaccacaa tatatagtat gtgcgacgaa atcctgtcaa tccgtcatca
    12661 tacatttacc tgtgttctat tcacaagcat ccattgttgt gaaaatagca gagactcttt
    12721 cctataggtt aattggcgat aaagctttca aagcgtttca gcaatcacat atgtaaaaaa
    12781 taagtgcaat agataacaca tcacactttt ctgaaaataa gaactttttt ctaaaagttc
    12841 tttttccacg agggacaaat atttctaatc taaaaacaag tccgtttttc agaaaatact
    12901 ttttttgcgt atttctttca atttttcaaa tcagtgttga ctttttgcag agaatgtgct
    12961 ataattattt tgttatcata cggcggtcaa ttgcacaaaa tgtgcaagcc gctaaaataa
    13021 aggagttatt attatgaaga atttgaagaa agtcgcagcc gttctggctg ctgcaatggc
    13081 aatgaacgct gttgctgtta ccggtgcatt ttccgcatct gctgccgacg atgcagacat
    13141 catcgcaaac gtttctctga agggacagat gggcggctat cagtactggg gcgaaggtga
    13201 tgaaagaaac acagacgaca tcaccagcac accggctaag atcacaaatg aagatggtca
    13261 gtacactgca acctggaaca tcacaggtga cggtaccggc acaatcgaat tcctgatgct
    13321 ggaatttgat tctgttgatg acaacttcat cactgctgac acctatccga aaatgagcgt
    13381 taaggttgat tctgtaaaag tagacggcga aactgttgat tacaagggtg ctgaaacatc
    13441 gatcaataca gcttactatg cagacgataa gggaagcact cgtgtatatc tgactgatac
    13501 atggggcgtt tctaaggctg gcaagatcaa cgatctgccg aaggataccg ctgttatgca
    13561 gagcatcgaa gtaaccttta gcgttgcaaa tctggaaacc gctctgccgt acggtgacgt
    13621 tgaccaagat ggcaatgtaa atacaaacga tctgctggat ctcatgatcg aagttgcaca
    13681 gaagggtgca ggcatggaag caacatatga tgactacaag tttaagctgg ccgacctgga
    13741 tggaaacgga aagctggata cttccgatat cttctatctg atgctgcaca ttgcacaggc
    13801 tggcgcaggt gtaagtgttg acccgtatcc ggtagaagca gcataagcat aacttaaaat
    13861 tctatttttt tccaaaacag aggaa
//
LOCUS       DAWENO010000043        13779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1842_length_13779_cov_8.681944, whole genome shotgun sequence.
ACCESSION   DAWENO010000043 DAWENO010000000
VERSION     DAWENO010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_13779_cov_8.681944"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1507..1541
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3291..3300
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4138..4147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6629..6638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3128 a         3836 c         3321 g         3429 t
ORIGIN      
        1 ccgtgggcga ggacagcttt ttcagattgg acagcgactt ttccgccttc acggtctgca
       61 ccgttcccag aattgcattc atggtgataa cggcaatgat aacgataaag ctttccaggt
      121 ctccggtaaa cgctgagatc agcgccgcaa taatcaagat gataaccaga aaatccttga
      181 actgttccag aaaaatcagc catgttggcg tcttttttgc ctcagcgatg gcatttttcc
      241 cgtattgttc cagatgcttt gccgcagtct cggcggacaa gccctctgga caggcatcca
      301 acttctgata gagttcttcc ggtgtttgct gataatgttc gttcatacac aacatccctt
      361 tcctttttgc agctggcgaa cggcgtattt cgggcagccc tgaacagagc gctgtccaaa
      421 agcacaaaaa cagaaaacgg cgagacctta cggaaccgac agctatcctg tccacgtgct
      481 ccgtaaaagt ctcgccgttt agatatgaag cgggtcgttt cacaacaacc gtactgacgc
      541 cgcataaaca gggacaagcc ccgccagcta ctctctttta ctctattatt atagccaatt
      601 ccggctatct tgtcaagaga tgcacaaaaa attcacaaat gaaaaagcgg cagcatctaa
      661 aacgccgccg cttttttatg actgcatcag aaacaattct gtgcaaattt gattagtcgt
      721 ttaccttgcg ggtataatcc ggcaggagct tcggagaaac ttcgtccggc tggatgccag
      781 cgtctgccag ttccttttcg aactttgcaa cgtgatccag agtcagattg ccgacttcaa
      841 cagtctttgc ctttgcagca gcgtacttac cagcgttacg gccgaatacg ataacgtcca
      901 gcagagagtt acccatcagt ctgttttcgc cgtgtacacc gccggcagcc tcacccgcaa
      961 cgaacaggtt ttctacgcag gtctggctgt ctgtgccgac cttaacgccg ccgttctgat
     1021 agtgcagtgt cggatagatc agcatcggct gcttccggat atcaatgccg tacttcagga
     1081 acattctcag catagccggg atgcgctttt cgatggtgcc ttcgccgtgg atcagatcga
     1141 tcatcggaga atccagccat acgccctgac cgccggtcgg tgtgttgacc tgcttgccac
     1201 gacggcactc acggataacg gtagatgcgg taacgtcacg agtctccagc ggattcatga
     1261 atacttcgcc gtctacgtta accagctttg cgcccagaga acgaaccttt tcggtaacca
     1321 gtgcgccgaa gatctgttcc gggaatgcag caccggtcgg gtgatactgc agggtgtact
     1381 gctcacgcag cggagcacca acacgataag ccagaaccag accatctgcg gttgcaccgt
     1441 agtggttgga agtcgggaag ccctggtagt gcattctgcc tgcaccgccg gtagcaatga
     1501 taacaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctttgcctt tgcgatcagg
     1561 atctcgccgg tttccatgtt catcagaaca gcacctgcgc agtgaccctt gtcgtccata
     1621 atcagttcga ttgcagaagt aaagtcaaca accgggatct tgcggttcag cacttcgtca
     1681 cgcagggtac gcatgatctc tgcaccagaa tagtcctttg cagcgtgcat tctcttgcgg
     1741 gatgtaccgc cgccgtgtgt tgtgatcatg ttgccttctg cgtccttgtc gaactcaacg
     1801 cccagatcct ggagccactg aatgccgtcc ggagcctcgg taaccagctt gtaaaccagt
     1861 tccttatccg cagcaaagtg accgccgcca aagcagtcca ggaagtgctg tgccggagag
     1921 tcgttcggct tgtccgcagc ttggatgccg ccttctgcca tcatggtgtt tgcatcaccg
     1981 atacgcagct tggttacgat cataaccttt tcgcctgcgt tgtctgcctc gatggcagca
     2041 gatgcaccgg caccgccgcc gccgataatc agaacatcgg tctcatagtc cgggcaggac
     2101 agatccggca gattgtcctt cagacggctg tcggactgga gcatttttac cagttccttc
     2161 ggagcctttt cgcccttgtt cggaccaaca gacagcacgt caaactgatc ttcacgatag
     2221 tccgggtgat tttccttcag gacctgatcc ttttcctctg cggtcattct tctcggttcc
     2281 attgctgcat tttctgcacg ctttgcttca accttcttta tggattccag catttcttcc
     2341 gtatacatga taccgcacct cccttacttc tcaatctcac gattgttata cttttcacga
     2401 atctcagcat cgctcagtgc catatttgca cggattgcct cttcgcactt gccctcttcg
     2461 atctccttga cacgatctgc cagatgctga catttcggtt caatgtactt accgttcaga
     2521 cggcgagcca gcatagcaac ctgcggatga gagatgccag ccggacagcg ggaagaacat
     2581 acgccgcaca ttacgcagtc gaaagactct tctgcgcact ttgcgtactc tgcacgctgt
     2641 gcatatgcga tatactgcat aacattcaga ccctgtgtac aagcgtttgt acaagcgttg
     2701 cagccgatgc aggaatagat ctccggatag ttcttcatca taacggtctc gtccggacga
     2761 acctcgttga tgtcgtagat ttccttaatc agcgggaaga acggcagctg tgtgatgtac
     2821 ataccctcaa cgacctgtgt ggagcaagcc agacaggtat ggagttctct gtcacccttc
     2881 acccgataga tcgttgcaca agcaccgcag aaaccattac ggcagccgca gccacgaacc
     2941 agcttatagc cagcgtattc caaagccttc atgatggtca gactggcagg aacctcgtac
     3001 ttcttgccca tcacgaacac attaaccatc tgttccatgt gaattctcct ttaccattaa
     3061 tattcattcg gcagttcgga cagctcatca aagcggaata ccggaccgtc cttgcagaca
     3121 tacttatcac cgatattgca gcggccgcac ttaccgatac cgcacttcat gcgcagttcc
     3181 attgtggtat aaacctgttc ttcctggaag cccagttctt tcagaccgtc cagagtgaac
     3241 ttaatcatga tcggaggtcc gcagatcaga acagtacggt tcagatccgg nnnnnnnnnn
     3301 gaagttcttt cccagcggac ctctgactgt cacttcctga ccaacctcca tcatatggag
     3361 ccagctggtc agacagccgc actttttaat ggaaaattcc atgaattcct tgtttgtcgg
     3421 agaagatgtg atagagaaca taccttcgcc gatgcccgga atggagacca tcgcacactg
     3481 accgggcata tgctcgaaca gcttgtttcc atccagacca accacacgga atgtctttac
     3541 atcaggggta tcctgacgga tgtcagttac gacgccgact tttggaatca atgcttcttg
     3601 tctcatgcgt taccctcccc taaagtcttc atgaccttga caatgttcat ggaaatcggg
     3661 cagctcttca tgcagcggcc gcagccaaca cagccgaata cgccttcatt gtttgccgga
     3721 tagtaaacca gcttgtgcat gaaccgctga cggaaacgct cgacctgtgt cggacgaggc
     3781 tgaccgccgg ctgtcttggt aaagtcggag tacatacagc tgtcccagca gcggaaccgc
     3841 tgaacgccgt gacctgtgtc atagtcacgg atatcatagc actggcaggt cgggcaaacg
     3901 aaggtacagg taccgcagcc caggcaagcc tcagaaagtc ctgcccactt atcggagttg
     3961 aacagttcca tcagcttgtc tccgccgaag gaatctgtgg acagattttt cagcggcagc
     4021 ttttccagaa ttgcctttgt ctttgccttg gattcttccg cagcatcgtc tgcggcatct
     4081 tccagcatag gcagggatgc cagcagcgcc tcaccctttt cggtgttcgc ctgcatcnnn
     4141 nnnnnnnttg tcataactac gccatgctcc cggcggttct gatagaaggt atctaccggc
     4201 tcagacagat atacgctgtc caggatctta aagcttgccg catcgcaggc acgaacgccg
     4261 aaaatcacga aatcttcgca ctcggtccgg atgtcatcca cttcgatctt cttgccgttt
     4321 accttaaagt tgaccatatt ttctacctgc gggaagaaaa agtcctttgc gctgcgaact
     4381 gtattgacag cctcagacgc atactttgtc ttcggtgtcc atacggtgta ctttgcactg
     4441 cgcttttcat cggtgctgtc agcaaccggc agatagagtt tctttgcttc tgcgatggca
     4501 gcgaacaaag cgtccagatt ttctcgagat acttttttca ttatttgctg cctcctttat
     4561 aaaccacatt cggctcgcag tcttccttgg tgaagttcac cagcggcgca cggctgcctg
     4621 cctctgcacc tgcctgatat tcaccgtaat aggtgtcgat atccttgatg aacttccggt
     4681 tcagcagatg gagcggaatg tgctgcggac atactctgga gcattctccg cagtccgtac
     4741 agcggcctgc aacgtggaat gcacggatga tgtggaacat attctcttcg aaggagtctg
     4801 ctgctgcctt ctgagaaacg ccggatgccg ggttatcaaa aacgcactgc tcacagctgc
     4861 acgccgggca gatattgcgg caggcattac agcggatgca gcgggacagt tctccacgcc
     4921 agaacgcata gcgctcgtcc ggagtcatcg cctccagctt ttctaccata tcgaaacgac
     4981 cggactccgg attttcttct ccgttttcgc ccagcatttc gtcataaaca acaaccttat
     5041 ggctcttgca gacttcgcag cgttccagca ggcagtcctt cttggctacg ctgacttcgc
     5101 cgtacatggt gtcaaccttt acggtgtcgc cgtcggtagt gatgcccagg atgccttctg
     5161 cgccggcgtc acgtaccttt tctgcatcca gcttgccgtc acacgggata ccgatgatgt
     5221 aaacgttttc acgcttgatg cggtgttccg tcagcagctg gttaaagctg taggaatcac
     5281 agggtttcag gaaaaccagt gtcttgccgt ccagcttaga cagcttgatc agatacttgg
     5341 acaggttgct gccacagaaa tcgtgatata caaaatcttt ttccaaagct tccgcagaat
     5401 cgaacacagc cggtgtggga tcatagacaa actcaccgtt tttccagccc agcacacgaa
     5461 caactgtacc gtctgccaac agctcggagg ctcttttcat catttcgctt tgcatctcag
     5521 atcctccagt ctcttgttct cgcccagtgc agttactgtc tctacgaact cattcatagt
     5581 tgcagcgaac tttgcacctt ctgcggcgga tacccattct acacgggtac gctctttctc
     5641 gatgcccaga taatccagca tggagaacag cagtgtcatt ctgcggcggg catagtagtt
     5701 accagtggta tagtggcagt cacccgggtg acatccgcag aggattacgc cgtctgcacc
     5761 acgctggaac gcacgcagaa taaagagtgg attgacacgg caggagcaag gtacacggat
     5821 gatcttgaca tctgccggat aattcgagcg gtttgtaccc gcaaggtctg cacctgcata
     5881 agaacaccag ttacagcaga atgcgacgat tttcggtttg aattcctgat ttacttgcat
     5941 attgcatcta cctccgccat aatctgaccg gttgcgaatc ccttcagatc cattgcgccg
     6001 gacgggcagg caacggtaca agcaccgcag ccctggcata ctgcctcatt gacaattgcc
     6061 acacgacgga tcatcgagcc atcctgccga cggctgaagt ccttctcctc atacgtaatt
     6121 gcgccgtacg ggcagacatt tgcacaagac gagcagccat tgcacttcat gggatccgag
     6181 tgagatacgc acgggttgca ggacagcttg tcctttgcca gcagaccgat gaccttcgac
     6241 gcagcagcgc ctgcctgtgc aacggtttcc ggaatatcct tcgggccctg gcagacaccg
     6301 gagaggaata caccggcagt cggagactca accggacgca gctttgcgtg tgcctcagta
     6361 aagaagtcgt tggtatccat gctggctgtc agcatagttg ccagcggacg agcgctcttg
     6421 tctgcttcga ttgcagcagc cagaacgacc atgtcagcct tgatgtgcag ctgttcgttg
     6481 gaaatcagat cagatgcctg aacatccagt gtgccgtcag cctgcggtgc aaccttgccg
     6541 accataccct taatatagtg tacgccatac tgctctactg cacggcgata gaactcatca
     6601 tagttcttgc ccggggtacg aacgtcgann nnnnnnnntg tagaatacat aaacgtcagt
     6661 atccggatat ttttcacggc acagcatagc gtgctttgcg gtatacatac agcagatctt
     6721 ggagcagtat tccttaccgc agccatgctg agaaccggat tcacggctgc cgacgcactg
     6781 tacgaatacc agtgtcttcg gctgcttgcc gtcggacaga cgctgcaggt gaccatcggt
     6841 cggaccgtct gccttgagga ttctctcgaa ttccagagaa gtcacaacat ccttgctctg
     6901 ggaatatgca aattcatcat agccgtcgat gttgatcgga ttataaccag tagccgcaac
     6961 gattgcgccg tacttttctt ccacgaaggt ttccttctgc tcatagtcga tcgcaccggc
     7021 agaacatacc ttggaacaca gaccgcactt gcccttggtg agcttgatgc agcgtgtgga
     7081 gtccagaaca gctacgttcg ggatcgcctg tgcaaacggg atgtaagcag cgtgacggtt
     7141 gctcagtccc aggttataag catccggaac attctttacc ggacactttt cggtacaagc
     7201 gccgcatccg gtacatttat cttcatcgac aaaacgagcc ttgcggcgga tcttaacgct
     7261 gaagttgccc acgaatccgg tcacagcctc tacttcactg taggaataga tagtgatctt
     7321 gtcattctgt gcgcattcta ccatcttcgg agtcagaata caggaagagc agtccagtgt
     7381 cgggaatgtc ttatcgatct gtgtcatctt accgccgatg gtgggttctt tttctacgat
     7441 atcgaccgga tagccagcct ctgcgatatc cagagcggtc tggatacctg cgataccgcc
     7501 gccgataacc aaagcacgct tggttaccgg gctttcgccg gcgatcagcg gtgtattcag
     7561 caaaaccttt gcaatggctg cacgtccgag gacgattgcc tttgctgtag cctcctggat
     7621 gtccttatgg atccatgaac actgctcacg gatgtttgcg acttccagca tataggggtt
     7681 caagccggct gctgaaactg tcttacggaa tgtcgcctcg tgcatacgag gagaacatga
     7741 acatacgaca acgccctcca gctgatactc cttgatcgca tctttaatta tgttttgacc
     7801 tgcctctgag cacatatagg gatagtcggt cgcaaaaacg actgccggct cttttccaag
     7861 agtctcagca acggttttta catcgacggt cgccgcaatg ttcgtaccgc agtggcatac
     7921 aaaaacacct attctccgca tttttatgcc tccttatttg ctgtatttcc ggaatgcgct
     7981 gacgattgcc tgctgcggca aatcatcatc cagcatttcg ggaatctgct ccgatttcag
     8041 gtgattctgt ccctgctgac ggataaccgc cagtctgact gcttccacca gttttgccgg
     8101 ttctacgtca cgaggacagc gttctacgca ggcaaagcag gagagacacg tataaatgga
     8161 cttggatgcc atcagctttt cgatgtcgcc cttttccacc atgtatacga actggtgcgg
     8221 atgatattcc attgcatcat aggacggaca agtaccgctg cactttccgc agcgcataca
     8281 tttcagcgga ttgacaccgc tggtcgtcag aacgagttct ttcaaatctg ccatttccgt
     8341 tcctccttat ttcagaccga gcgcttctgc gagcaactca gtaaagtata cgaccggcac
     8401 atcagaaacg ccggagttct tctggaggtt atacagacaa agcggacagg cggtgatgac
     8461 catttccgca ccgttttcct tggcgctggt cataaccttg ttgcttctgg actgtgccac
     8521 ggacttttca tccagtgtca gatatccgcc gcagcactca ttgcgcatcg gatacagcac
     8581 cggagtcgca ccgatcgcac ggataaagtc ttccaggatc ttcggatttt ccggattatc
     8641 catctgcatg gtctttcccg gacgcagcag aaggcatccg taataagcgg cgatcttctt
     8701 gcccttcagc gggttgacca ctttcttttt cagttcatcg aaaccgacct tgtcccgcag
     8761 cacttccaga taatgcagca cttcggtctc gccctgatac ggctcagcca gcttcagata
     8821 attgtttgct ttcagaacga tgttgtcatc agtctgcata tcatgattga cctgcttgat
     8881 gacgttatga caggcagagc agagggtgac cagcggctgg cccagttcct ttgcttttgc
     8941 cagcgcacga atggatgaga gcttggttgc gatctcattc ttcgccattg gatacacacc
     9001 gccgcagcac tgccaatccg gaagttcttc cagtgtaaag cccagtgctt cagcagagat
     9061 ccgggcatag tgatcgaggt ctttcgcctt ggtctttaag gtacagcccg gaaaataact
     9121 gtatgtcata gcaaaactac ctttccttcc tgtttttttg cgccgaggcg caggtggttc
     9181 agaccatctg ttccggatgg aacacttcgt cgaaccgttc agctgtcagg aatcccagct
     9241 caacgcaggc ttccttcagc gaaatgttgt cacggaatgc cttcttcgca gtctttgcgg
     9301 cattctcata accgatatag gggttcagcg ctgttaccag catcagagaa ttgtgcaggt
     9361 tgtggtgcat cttttcacgg ttagcgcaga tgccgactgc acagttcttg ttaaaggaga
     9421 tgatggactc agccaggaga cgagcagact gcaggaaatt gtaagcgcag accggcataa
     9481 atacgttcag ctcaaagttg ccctgagaag ctgccatacc aactgccacg tcgttgccca
     9541 ttacctgaac agccaccatg gttgcctgtt cgcactgtgt cggattgacc ttgcccggca
     9601 tgatggacga acccggttcg ttctccggaa tggtgatctc acccagaccg tctctcggac
     9661 cgcttgccag ccagcgcacg tcatttgcga tcttcatcat atctgctgcc agtgccttca
     9721 gagcaccgtg tgcaaatacg atctcgtcct tgctggtcag agcgtggaac ttgttggatg
     9781 cggtgacgaa ttccttgccg gtcagttcag aaactgcctt tgcaacttct acgtcaaatc
     9841 cctccggtgc attcagaccg gtaccaacag cagtaccgcc cagcgccagt gtcttcaggt
     9901 acggcagcgc agaaacgagc atctcgcgat cacgctcgag ggatgtgcgc catccgctga
     9961 tttcctgaga gaacttgatc ggggtcgcat cctgcagatg ggttctgccg ctctttacga
    10021 tgccctcatg ctcttcttcc agacggcgga aggttgccac caattcgtca attgccggca
    10081 gtaccttatc ctctacagcc agaacagcag agatgtgcat tgcagtgggg aatgtgtcgt
    10141 tggaagactg gctcatgtta acatcatcat tcggatgcag gatcttcttg ccagcgactt
    10201 cgttgccacg attcgcaata acttcattgg cattcatgtt ggactgcgtg ccgcttcctg
    10261 tctgccatac gaccagcggg aagtgttcat tcagactgcc ggagatcacc tcgtcacagg
    10321 cagtggaaat tgcagaaagc ttttcctctg tcatgcgctc cggacggagc ttgtggttcg
    10381 ccattgcagc agcctttttc aggataccga aagcgtgtgt gatctcacgg ggcatggttt
    10441 cgatgcctac accgatcaga aagttctcgt ggctgcgctc tgtctgagct gcccaatact
    10501 tgtcggcagg tacctttacc tcgcccatcg aatcgtgttc aatgcgatac tccatgttca
    10561 tctctcctat tttgaaaatt cggactacgc cgtgcagacg gcacagcatc gtctctggtt
    10621 ttgatatcgc ctgtgacaac gcacaggcat tccttcgggt ctgtatcacg cagaccccgt
    10681 tgtgtccggc aaaaaggctg cactgccatt ggctgcagtc tgccagtccg ggctgcttgt
    10741 ccgcagtctc acctgcgaaa ccacagcaca gcacaataca cctgtaccat gcagcacagg
    10801 tgtgaagcgc agacagcaca gctttttcgc cgtcaaaccg tcctgtccga acgcaatcat
    10861 gatcgggaca atccccaaaa acaaacgctt tttccaggga tatcccggtg gaacagcctg
    10921 cggctgtcca cgacagaaaa atcagtgttt tccgtgaaaa taccgtcgta tccgcacgaa
    10981 aaaacagcat gaaatgctat ctactatatt atagcatatt ccgcccgatt tttcaagaga
    11041 caatcgcaaa ttttgtatga acaagcggca cttgcacgaa gagtgtcgat tcccggtcaa
    11101 gccaaaatct gccgccgctc agctattaca caccattttg cattatacca ttttcctgtg
    11161 aaaaaatcaa caacttggaa tacgactcat gattaacaaa gtttttgctc agatcacggc
    11221 gtatttcgct gcaattcctt tcggattctc atgtttcggc aaatgcaaac cgatattttt
    11281 tcagttagta ttgactaatc tttgaattga aaaaaacatc gtatttttga ggcgatacca
    11341 cacaaggctt acagccggac tttgtgcagg attgccctag gcgttttttt cgcagccgac
    11401 ccttgccttt tgagaaaaag cgtggtataa tgcagttgtt ccgaaaaaca gaacacggag
    11461 gtaaacccca atggaatctt ttcatcaccg gctgcatcag gcaatgcagc agaaacatat
    11521 cacgcagtct gatctctgcg ccaaaaccgg catttcacgt tcgaccatga gccagtactg
    11581 ctccggggca tttcgtccca aggacagcaa gctgcacgcc attgcaaagg cactgggcgt
    11641 caaggaggca tggctccttg gctatgagag cgaagaagaa ccatcgtttt ccctggagga
    11701 ctatccgaat ctccgaccca tccgactgaa acggtttccg cttctgggcg agattgcctg
    11761 cggcgaaccg atcttcgccc aggaggatcg ggagactttt ctcatcgccg acgcagagat
    11821 cgccgcagat ttctgtctgc tcgcccgtgg agacagcatg atcggcgcac ggatctacga
    11881 cggagatgcc gtctttatcc gcagccagcc catggtggaa aacggcgaga tcgccgctgt
    11941 catcattgac gatgaggcaa cgctgaaacg ggtctactat gacaagacca atgccatcct
    12001 gcagctggtg ccggaaaacc cggcgtattc tccgctggtc tacgtgggag cgcagctgga
    12061 cagcatccgc atcctgggaa aagccgtcgg gtttatgagc cttttttgag tatctgttcc
    12121 ccgttcttat gggaaatcga aaagccgttc gtacagatgt acggacggct ttcttcttgt
    12181 gtgttggcat atctcagagc agcgggctga tggtgcgcag aatcgcctgc cagagcaagc
    12241 tgtggcggcg gcgcttgcag tcctgcaagg tcacacggcg gctcttctcg atggtcacca
    12301 tgcagtcgat cttcagcttt tgcagagcgc tgcacccggt cagcagcacc gcatcttcca
    12361 tgtgcagata cagactgcgg tagtccatgt tgatcgttcc cacaacgccg atctcatcgt
    12421 ccaccaggta actctttgcg tgaatgaatc ccggcgtata ctcatagatg cggacaccgg
    12481 cacggatcag cggctcataa tacgaacgtg tcagcgaata gaccatgcgc ttgtccggga
    12541 ttcccggcgt caccagccgc acgtctatgc cacgctgtgc cgccatgcag agtgcgtgct
    12601 gcatctcgtc atcaattgcc agatacggcg tgaaaatata cacgtaattt ttcgcctggt
    12661 tcagcagttc caggtacaca tcctgcgcca gggtctcgct ctcaaacggc gaattgcagt
    12721 acggcagcac atagccgtct gtggggaacg gctccggatg gtagcggtgc ggcaggtatt
    12781 ttttgcagtt gtcattggtg ttgttgaacg cattccacag ttccagacac atcagcgtaa
    12841 agctgtacgc cgcctcgccg tagatccgca gaccggtatc cttccagtgt ccgaaccgtt
    12901 tcttttggtt gatatattcg tccgccaggt tgaatccacc cgtataggca atatagccgt
    12961 cgatcaccgt gatcttccgg tgattccggt tgttcatcac cgcagaaagc gctggtcgga
    13021 acggattaaa cgccatgacg tggatgccgg catccgaaag ctccttgcgg aactgtttcg
    13081 gcaggcatcc cacgctgccc aggtcgtcat agacaaagcg cacttccact cctgcggctg
    13141 ccttttcttt cagcaccgcc ttgatggaat cccacatcac cccgtctgcc acaatgaaat
    13201 attccatgaa aatgtaatgt tttgcccttt tcagatcttc cagcagatgc ggatacgcct
    13261 catctgcaag cgcatagtag tccaccttgg aattctggta gatggggaat ccacagcttt
    13321 ccaggtaaaa gctggtctca gccattcgct gcggcagtgc agccgccgtg catcccacct
    13381 ccggacgcag accaatgacc cgtctgccgt ggtgcagctg acggcgcatt ttccgggacg
    13441 gtcgcttgtc gccgaacagc agatacatca gaccgcccag caccggaaac gtgccgataa
    13501 acagcagcca caggatctta tacgaaggat tccccgactt gttgatgata taaagctcga
    13561 tcagcaggct cagcgccaca aatccggcat tgatccaggt ggcatactgc gtcagccgca
    13621 gaaacaggat caaaaaccac gccagctgaa tcagaatcaa cagtccgata atcacaagcc
    13681 ggctggttag aatactgagc agttttttca gttttcggcg catcacatca cacatcctct
    13741 ctttttctgt ccgattctta ttgcgtacat ttaggattt
//
LOCUS       DAWENO010000044        13660 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1869_length_13660_cov_15.050423, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000044 DAWENO010000000
VERSION     DAWENO010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_13660_cov_15.050423"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3204 a         3450 c         3846 g         3160 t
ORIGIN      
        1 atcatcattg gtgcggtatt gtttgccgtg gcaatggcag tcacccatct gctgacgctg
       61 ctctggtggg cagagctgct gatcttcctg gcagtgtatc tggtgccggg attcagtgtg
      121 ctggaaaagg ctgtgcggaa cattgcgcac gggcaggtgt ttgatgagaa ttttctgatg
      181 acgatcgcct ccattgcggc gtttgccatc gggcagtatc cggaggcggt ggaagtcatc
      241 ctgttctacc aggtgggtga gcttttcgag aagatcgccg taggacgttc ccgccaggca
      301 gtgtccgatc tgctggatct ctgcccggat gaggcgacgg tacttcggga cggcaagccg
      361 gaaacggtgc tggtggacga agtggaagtg ggcgaacaca ttctggtgcg ccccggcgaa
      421 aagatcccgc tggatgcgga ggtctttgag ggacatacca ccatcgacac cgctgcgctg
      481 acaggcgaat ccatgccggt gacagccggt gtgggcgatg cggtttgcag cggctgtgtg
      541 aatctgtccg gtgcggtgga actggtcgtt accaagccgg cgtcggaatc cactgcctcc
      601 aaaatcatgg agctggtgga agaatccgcc tccagcaagg caaagacgga gcagtttatc
      661 actaagtttg cccggttcta tacgccctgc gtggtcattg ccgctgtaat tctggcagtg
      721 ctgccgccgc tcattacggg gcaggcgttc tccggctgga tctatcgtgc gctgacgttc
      781 ctcattatct cctgcccatg tgcgctggtc atctctgtgc cgctgagctt tttcggcggt
      841 atcggctgtg cgtccaaaaa cggcgtcctg gtaaagggca gcaactatct ggaggcgctc
      901 tctcatgcgc agacggtagt catggataag actggtacgc tgaccgaggg aaagttccat
      961 gtgacggaac agaacccggt gggcgtgacg gcagaggaac agctggagtt ggcggcgctg
     1021 gcagaatcag cgtccaacca tcccattgcg cagtcgctga aggatgccta cggcaaagcc
     1081 attgacgcag agcgtgtcac ggatatccag gagatcgccg gtcacggtgt ggctgccagg
     1141 gtggacggca gacaggtgct ggcaggcaat gcggcttatc tggaagatgc cggactccag
     1201 ccggaaacac cccacgccat cggcacggca gtgcatctgg cagtggacgg cgtatatgcc
     1261 ggatatatcc tcattgcgga tgcagtgaaa ccggatgcgg cacaggcagt gcaggcgttc
     1321 cggaaggctg gcgtttccac ggtggcgatg ctcaccggcg acagcgaatc tgcggcgaag
     1381 gctgtgggcg atgccctgca gctggacgaa gtacacgcca agtgcctgcc ggctgacaag
     1441 gtgaagttca tgcaggaata ccgcaccaag ctgtccaagg gcagaacgct ggtgtttgtg
     1501 ggcgacggca tgaacgatgc gccggttttg gcacagtcgg atgtgggcgt tgccatgggc
     1561 ggtctgggtt cggatgcagc cattgcggcg gcggatctgg tcatcctgga cgacaagccc
     1621 tcgaaactgg ggctggcgat ccacattgcc aagaaaacca tgggcattgc acggcagaac
     1681 atcgtgtttg cactgggtgt caaggcgatc gttctggtgc tgggtgcgct gggtctggca
     1741 catatcgggg ctgcggtctt tgcagacgtg ggcgtttcgg taattgccat tctcaactcc
     1801 acacgggcaa tgtggacaaa agtgaaataa gcgcttgtga aaagcttttc gggcagatct
     1861 ccggtcgggg gtctgccttt ttgtaaaatg tgcaaaaaag tggaaaaaaa tcttgacacg
     1921 gctcgtctta ttgtagtata atagaagtaa tcaccattct ttccaaaggg tggcgtgcag
     1981 gaaaaatgcg gcgcagcagg attgcagctc cattttgaaa tgcagaaaaa tctgaacaga
     2041 aaaggagcat ttgcatatgc tgctcgacaa atatttacca cgcatcgagt ttgacagcta
     2101 cgaggacttc aaagagaact atcgtgtaaa cattcccgat gactttaact tcggctttga
     2161 cattgtggac gaatgggcaa aacaggaacc ggaaaagaaa gcgctggtgt ggtgcaatga
     2221 ccacggcgag gaaaagacgt tcacctttac ggacatctcc cgtctgtcga accagaccgc
     2281 aaactatttt cagagcctgg gcatccggaa aggcacagtg gttatgctca tcctgcgccg
     2341 ccgctgggaa tactgggtat gcgctgtggc actgcacaaa atcggcgcca ttctgattcc
     2401 cggctcgctg cagctggcga aaaaggatct gatttatcgt ggaaacagcg cacagatcca
     2461 caccatggta tgtctggacg acaagtatgt cctggagcag gtggatgagg cacagccctc
     2521 tgtccctagc atccagcaca agatcgtggt gggcggctct cacgagggct ggcgcaattt
     2581 ccacgaggaa gtggagaagt tctccacaga gtttgcacgt cccaccggag acgccggcac
     2641 aaaggcacac gatctgatgc tggtttattt cacctccggc actaccggtg atccgaagat
     2701 ggtagaacac gactacactc atccgctggg gcatatcgtt acggcaaagt actggcagca
     2761 ggtgcaggaa aacaagctgc acatgagcgt ttcggattcc ggctgggcaa agttcggctg
     2821 gggcaagatc tacgggcagt ggatctgcgg cgcagtgatc ttcgcctatg atatggacaa
     2881 gtttgtgccg acccatctgc tccagaagat gcaggactat aagctgacca ccttctgcgc
     2941 tccgccgacc atgtaccgtt tcatgctcca ggaggatgtg gcatcctatg acctgtccag
     3001 catccagaac ttttccaccg ccggcgaacc gctgaacccg gaggtataca accgctggca
     3061 ggaactcacc ggcaaggaga tccgggaggg cttcggacag acagagggtt ctgttctgct
     3121 ggcaaacttc ccgtggatca cacccaagcc cggctcgacc ggcaagccgt ctccgctgta
     3181 tgatattgta ttgcagcacg atgacggtac gcttgccaag gacggcgagc agggcgctct
     3241 ggtgatgcag aatctgaaaa aggcatatcc caccggtctg tttgtgggat actacaagaa
     3301 cccggaaatg acacaagaga tcctgggcgg cggcagctat tgtccgcacg atgtgttcat
     3361 gcgggataag gacggctact actggttcgt gggcagaaat gacgacgtta tcaagtgttc
     3421 cggctatcgt atcggaccgt ttgaggtaga aagcgccctc atcgaacacc cggctgtgct
     3481 ggaatgcgct atcacagcgg caccggatcc catccgtgga caggtggtca aggcgaccgt
     3541 tgtcctcacc aagggctata cacccagccc ggagctgaca aaggaactcc agaaccacgt
     3601 gaagcatacc acagcgccgt ataaatatcc ccgtatcatc gagtacgtgg acgaactgcc
     3661 gaaaacactg ggcggcaaga tcaagcgtgc gcagatccgt aaggaagacc aggcagccag
     3721 cgaaaagtaa gaattttttt gatttgtata gagaaagcgt cgtgcagagg tttctgtacg
     3781 gcgctttttg cgtattcacg ccaaaaatca caaaaacaga ttgaaaattc caggcaaata
     3841 tgctataata gaatcggaga ttttttcgga tgcgaaatgc aaaatcaaaa aaggaggttc
     3901 gggagatgcg caaaagagga tgtgcttttc tttgcagcgc agtgctgctg accggggtgt
     3961 ttctgcggct gcctggggaa atgtggcagg tgcaggcgga gaccatagac acggcggcat
     4021 ttgcggaaga gactgcccgg ctgaccaacc agttccggca ggaaaacggg cttgcgccgc
     4081 tgaaaatggc gccggtattg ctggaactgt ctgcccggcg tgcggaggaa ctgaaagacg
     4141 catacggtca cacccgtccg gacgggcggg actggtttac catcgttgag gagtcctcgc
     4201 tggatgccaa ctgctatgcg gcggaaaatg tggcagccgg ctatgatacg ccggagagcg
     4261 tgatcgcagc gtggatcgat tccgatgcgc accgcaaggc gatgctggga gaaaactatc
     4321 agtatatcgg catcggggtg agccagctgg aggatgaccc gaatcattac tacacctact
     4381 gggaaatgct gctcatctca tcggaacagc ctctggaagg ggaatatgag ccgggcgaaa
     4441 cggcgaatcc cacggtttcg gaaaaaggcg tttccctttt gggggacgta gacctggatg
     4501 gtacagtgga actggcagat gctgtgcttt tgcagaagat actgctggga caggtgactg
     4561 gaaatgcggc gcagctggaa aacggagact gctgtgcaga tggtgtgctg gactgccggg
     4621 atgtgacggc gctgatgcag atgctggtat cgtaagacgg gagagagggg acagatatgt
     4681 cacgaattct ttatgtgctg gattatctca gccgcaatac ggatgcagaa aagggtgcaa
     4741 cgctgaagga tatccaggca tatttgctga atcagaccaa cgcaggctcg gtgtctacac
     4801 tgagcattca gcgggatatc gaccagatcg acgccatggg gtatacggtg gacatccgga
     4861 agggggcgca caacacgtcc tattatcata tgcgtgaccg tgggttcacc ttcaatgaga
     4921 tccgctttct ggtggattcg gtgtccatca acaagttcct cagcaacgcc cagaaacagc
     4981 gcctgttccg gaaattcgag gtgctgcgct cagagtcgga ggtgcgaaag ctggtgggac
     5041 gcattaccct cagcgaggtt gcgccgccgt cactggacct gctggacaat ctggaatgcg
     5101 tgcatcagat cattgcggaa aagcggaaaa ttcattttca gtacggcaag tataacatcc
     5161 accgggaaat cgaatactat gacaaaaagc ggaatctgat cccgtgccag gtggtgtata
     5221 ccagtgaacg cttttatctg aaagcggtct cggaggacac gggagaggtg cggaactatc
     5281 gcattgaccg gatgcggaag atcttgccag gagagaagat cgctcatttg ccaaagctgc
     5341 ctaaaccgga gggcttttcg gcagatatct ttgtgccgga ggtgtatgag gatgttcagc
     5401 tgcgtgttcg ccgggtgctg ctggacgata tgatcgaaca gctggggaac tatgcccatg
     5461 tgcgtctgga cgagaaagac ccggagcatt acatcctggt gcgtgccaga atgggcatta
     5521 gctacgggct gtaccgctgg attatgaagt acggcgccag catggaagtg cttgcaccgg
     5581 agagcctgcg gaaagaggtg gcacagcgca tccgggcggc tgctgcactg tacagcgaca
     5641 cagacagaga gtagtacact gcacaacaaa aaacggacag catttctgct gtccgctgtg
     5701 ggattctgtg gaatgattac gcctgtccac gttcctttgc cagggtgtcg tagcggtcca
     5761 tcatttcctt gatctccggg tcgttggtca gtccgtgctg catatgcaga tagaaccgga
     5821 tcgccggcag gtccttgttc tcgactgcct ctttcgccag ggcagtgatg acgccgacaa
     5881 cgtcctcgtt tgcgccgcac ttcatgccgt agtgtgcaaa cgcctcattg acctcgtccc
     5941 gtgtgggcgg tacaattttc ttcttggtga tggtgtggat gtggtgcagt tctgccggaa
     6001 ccatggggtg gtcggcatag atcaggcttt cgtagtagaa gcagactgca cggtcatagt
     6061 ccttgacctc gacgcagtaa aagcccaggt tgcagtagca ccgggaaagc tgtaccggtg
     6121 tcacagccac ttccagtgtc tccttgataa cggagagcag ctggtcgtgg tcacgcaggg
     6181 acttgtagca ttctgccagc tcgaagtatg cgtccgtgtt catggggtta tagcggatgg
     6241 catcgttgat gacacgggca gcctcttctg cacgtcccag ctccagcagg ttgtagccgt
     6301 ggagcagcac catctgggac aggtcaaata ccggacgcac cagctttttg gacgggcggt
     6361 agaagtacag atagagctga tgctccaggg tgttgcggaa gctgaggtat acctcagact
     6421 ccgtttcccg gtagttcatg cggatcttgg tgtacagcgc ctcagtcagc ttgaaggact
     6481 cgtcgatctt cttttcctgg atgagtttgt ttgcctctgc aaagacaacg tccaggcgct
     6541 tgccgtcaat gtacatcatc ttgttgagaa cttcccgctt gtcgtcgggc aggatctcat
     6601 atgccaggtc ggcaatggcg ctttcgatct ctttggcgtt ttttgcagaa cggtacttgt
     6661 ctgcctggga ttccagatag accatgtcta cgctgtattc tccggtcaga ccctcccgga
     6721 ttttttccat aatttcattg attgtcatag tatagctcct ttgttgccgg taattccgga
     6781 cggtatagtt ttattataca agatttctgg gataaatgca agtctttttt gtggattttt
     6841 tcaaagcagt accacacaga ggagagaaag gatacacagg tatgcaggaa attcttacag
     6901 cattggaaca gcggcacgat ctgccggacg aggcgctctg cgctctgctg gaaacggatg
     6961 ccatggatgg ggcgctgttt caggcggcgg accgcatccg ccgggaacga tacggcacgg
     7021 aggtatttgt ccggggactg atcgagttca ccaactactg ccggaacgac tgctattact
     7081 gcggcatccg ccggagcaac ggaaaggtac agcggtatcg gctgagccgg gaggatatcc
     7141 tcgcctgctg tacagagggc tatgcgctgg gattccggac gtttgttttg cagggtggcg
     7201 aggacgcata tttcacggaa cagcgtgtat gtgagattgt ctcggagata cgggaacgct
     7261 ttccggactg cgccatcacc ctttccattg gcgagtggga aaaatccagc tatgcggcgt
     7321 ttttccgtgc cggcgcaaac cgctatctgc tgcgccacga gaccgcagac ctggtgcact
     7381 accgccgtct ccaccccggc gcaatgtcgc cggaacaccg gaaacgctgc ctttttgatc
     7441 tgaaagagat cggctaccag gtgggttccg gctttatggt gggttcgccg tttcagacca
     7501 cggcgcatct ctgtgccgac cttcgctttt tgcaggaact gaagccggat atgatcggca
     7561 ttggaccgta tctcacccac ggcgacacac cattccggac acagcctaac ggcagtctgg
     7621 aactgacgct gcggctcatt gcagtgctgc ggcttatgtt cccgtatgcg ctgatcccgg
     7681 cgaccactgc cctgggtacc attcatccgc agggcaggga aatggggctg aaagccgggg
     7741 caaatgtggt gatgccgaat ctttctccgg tgtcggtgcg aaagctgtat tcgctctatg
     7801 cggacaagat ctgcaccggg gaagaatctg cccagtgccg gagctgtctg gaacggcgtg
     7861 tggcggtggc aggctatgcc atcgtgacgg atatcggcga tgtgcgccgg aactaaggca
     7921 acaccgcaca aagccgtcag gcgggctttg tgcggtgttg ccctaacagc aggatgaggt
     7981 gttcgaaaaa aatttgcata aaaagtattt gcatttttcg gaaaatatga tataataaga
     8041 aagaactgtg gcagcatggt acaaagccag tgcggtgctg ccggatgaga ttgcgtgggg
     8101 ctttctgctg aaatgacgtg ttttcggtga ttttggcgga gagtgcctgg tgtttggagg
     8161 gctttatatg agtcaggaac aacaaaagaa aacccggtct gcaaaaaatg cgccgcagaa
     8221 aaaacagatg cagaagcatc cgaatccccg ccgcccgaga ggttccggcg gaaagacggc
     8281 ggcacaggtc atcgtctccc tggttctggt ggcgcttgtg ggatatgagg gatttcatat
     8341 gtaccgctct ttcacaccca tctcacaggg gtcggagagc atggatctgg tgggcggcgg
     8401 cagcgagtct gaggctgcga cagaaccgaa aacggtctat gagaacgtct ccgtttccaa
     8461 cgaagatgtt cacgccggtg atctgatcct ggtcaacacg gataccgcat atgtggagga
     8521 gaaccccacg gagatcgtga gcatctacga ccacaagacg gacaactacc atgtcagcgg
     8581 tacagagacc agcctgcgtc agccggcgct ggatgcgctg aaccagatgc tggacgcctt
     8641 ttatgtggcg accggacacc aggacatgat cgtcatcagc ggctaccgca ccacacagca
     8701 gcagcaggag ctgtatgatg cagacctggc agagaccggc gagcagacct cgacccgtgt
     8761 ggcactgccc ggacacagcg aacacgaaag cggctatgcc ctggacttct ccctgtacac
     8821 cgacggggtg cagtacgact atgacggcac cggagaatat gcctggatca acgagaactg
     8881 tgcgcactac ggctatgtgc tgcgctatgc agaggacaag caggagacca ccggcatcca
     8941 ggcagagccg tggcactacc gctatgtggg acagccccac gccacctata tgatggaaaa
     9001 caacgtgtgc ctggaggaat accttaccct gctgaaaaat tacaccgcag atgagccgct
     9061 ctccatcacc aactgggacg gcgagatcta tcaggtatac tatgtggctg cggatacttc
     9121 cacggattcc acctatgtga tggtgccgcc ggatgcgaag tacaccatct ccggcaacaa
     9181 cagcgatgga tttatcgtaa cagtggacac tggcgagatc cagagcggag acgccgcagc
     9241 ggcagctgcg acaacgccgg aggacagcgc agcagcagat acggaaacag caacagaaac
     9301 cagtgctgcc gaataaatag gaaaaggtga tctatcatgg aaaatgaaaa gaaaccaacg
     9361 gaagagactt tgcagaaagc cggaaagggc ttgaaaagcg tagtcggtga gttcaaggag
     9421 tttatttcca agggaaatgt actggacatg gcggtcggct tgatcgttgg ctcggcattc
     9481 acagctatcg tgaactcgct ggtagacgac attctgatgc cgattatcgg cacagtgctt
     9541 gccggcatca acttctcgtc tctgggaatc acgatcccat ggggcaatca tccgtttatt
     9601 gccatcggca gctttttgaa cacggttatc acattcctgc tgacggcgct ctgcgtattt
     9661 ctgattgtaa aggtcatgaa tatgttccgc cggaaacagg aagaaaagcc tgcaccgccc
     9721 aagcccagca aggaagagat cctgctgacg gagatccggg atctgctggc ggcacagcag
     9781 ggaaaagaaa cgactgagac cgaggaaaag aaagattaaa tctgacatct tcttactatt
     9841 ctacatatag aaaaaacgct gttccacgtg caggaacggc gttttttcgt ggggattata
     9901 ttgtgtactg tggttattat gaaacctgtt ttcgttcttt caattcttgt tctagttctt
     9961 gtattgcctt tttcaattct tcggctgaca tttttttgat ttcatctggg ataattacat
    10021 tgtcatcaat aaacattttc atattatttt gcctcccaaa aatcaatttg gtaattttgt
    10081 ttgagtcttt ttgattatat catcattgca attaaaagtc aatgatgttt tattgagagc
    10141 ttatcataaa acaaaaagca ggagcagcca tattccaggc tgctcctgct ttcctttatg
    10201 gagaggtttc gttattttga ggatttctgt ctgtgttttt gtgtgccgcc gtgacagttg
    10261 caggcgttcg gacagccgcc gcaggtaccg ccgcaggaat gttttccctt tttgcggtca
    10321 ctccgcatgg acagaacgat cgcagcaatg attgccacaa caatgattcc gacaatgaca
    10381 cttcccatag aaatgcctcc ttttgaaaca tcaggtcaat ttgagtttat gcctttacct
    10441 taacgcccag cttgttgctt tccttatacg gacgaaccag caggtagatc attgcaataa
    10501 tcagagcgaa tgcaacgatc aggctgatga tgtttacaac accgttgccg gcagtcatca
    10561 gctgaccggt gaacaggttg cccagctggt ttacgatcag agcgattaca taagcgaaac
    10621 cgcactcgta accaattgcg aaccagaacc acttggtgtt gttcatctca cgcttgatcg
    10681 cacccattgc tgcgaagcac ggtgcgcaca gcaggttgaa gatcaggaag gacataccag
    10741 ctacaccggt gaatgcagct gccagatttg cataggtgcc gtcgccgccg ccgtacagga
    10801 tacccatagt tgctacgatg ttttcctttg caaccagacc ggttacggct gcaacagttg
    10861 cctgccagtc gccccagccc agcggagcga agatccagca gataccctta ccgatataag
    10921 ccaggatgct gtggctcatc tggtcctcag tcagcatacc gaaggaaccg tctacccagc
    10981 cgaagaagga agtgaaccaa acgaagatgg tggacagggt gatgatggta ccagccttct
    11041 taatgaagga ccagccacgt tcccacatgg agcggagaac gttgcccaca gtcggcatat
    11101 ggtatgccgg cagctccata acgaacggag ccgggtcgcc ggcgaacatc tttgtcttct
    11161 tcagaatgat accggaaaca acgatcgctg ccatgcccag gaagtatgcg ccggttgcga
    11221 tccatgcaga accgccgaag attgcgccgg cgatcattgc gataaacggc tgcttagcgc
    11281 cgcaggggat aaaggtggtt gtcataacgg tcatacgacg gtcacgttcg ttctcgatgg
    11341 ttctggatgc catgatgcct gggatgccgc agcctgtgcc gaccaggatc gggatgaagg
    11401 acttaccgga cagaccgaac ttacggaaga tacggtccat aacgaatgcg atacgagcca
    11461 tgtagccgca ggcttccagg attgccagca ggatgaacag taccagcatc tgcggtacga
    11521 agcccagaac tgcaccaaca ccggcaacga taccgtccag aaccagaccc ttcagccagt
    11581 ctacgcagtt aacagcgtcc agtccctttt caatcagtac cggaataccc ggaacccata
    11641 cgccataatc tgccggatcc ggttcgccct ttgcgaattc gtctgctgca gtggtcaggt
    11701 cgtccatgga agcggtttct gtggtaactt ccagtgtttc ttcatcttct acatcataag
    11761 agaagtctgc gttgtaatcc ttttcatagt tcaggatcat gtcctttgcc gcttcgccgt
    11821 caaaggtgtc tgcttctgcg tcgatgtagc tttccagatc agcggtatcc acgccctgat
    11881 cgcccagata agccacatag ccgtcaataa tggcattggt gtcgccgtat tcatctgctg
    11941 catcattata agagcttgtg ccgatgccca gcaggtgcca gccatcgccg aacagtccgt
    12001 cgtttgccca gtctgttgcg gcagaaccaa cggtaaccat ggagatgtag taaaccagga
    12061 acatgataac tgcgaagatc ggcagaccca gccaacggtt ggtaacgatc ttgtcgatct
    12121 tatccgatgt tgtcatgccg gtcttcttct tcttcaggca gtccttgatg atggatgcga
    12181 tgtacagata acgctcgttg gtgatgatgc tctctgcgtc gtcgtccatt tccttttcgg
    12241 cagcctcgat atcctgttca acgtgctttt tggtagcctc cggaatgttc agctgttcca
    12301 gaaccttgtc atcacgctcg aagatcttga tggcatacca tctctgctgc tcttccggct
    12361 ggtcgtgcag gcaagcctct tcgatatgtg caatagcgtg ttccacacag ccgccgaact
    12421 tgtgctgcgg taccatggga accttgctct cagcagcctt cattgcctcc ttggcagcct
    12481 cgtcaatgcc ggtgcctttc agtgcggaga tctcaacaac cttgcagccc agttcctttg
    12541 ccagctggtc gatgcggatc tggtcgccgt tcttgcggac aatgtccatc atgttgatgg
    12601 ctaccactac cggaatgccc agttctacca gctgtgtggt gaggtacagg ttacgttcca
    12661 ggttggtacc gtcgatgatg ttcagaatgg catccggacg ttcctggatc agatagtttc
    12721 tggcaacaac ctcttccagg gtgtacggag aaagggagta gatacccggc aggtctgtga
    12781 tgataacatc tttatgacct ttcagcttac cttccttttt ttctacggtt acaccaggcc
    12841 agttaccaac gaactggttc gaacctgtca atgcgttgaa caatgtggtt ttaccagcgt
    12901 tcgggttacc ggctaatgcg attttgatac ccatttgatt caatcctttc gtacatttta
    12961 tctgttagtg attgctaatt agtagttact aacttgtgac caaaaaaata attctgccgt
    13021 aaaatgaaga ggtgaatcta ctgaaacagc agatccacag gttttttatt ccacttcgat
    13081 cagttcggca tctgctttcc gcagggacag ttcgtaacct cttacggtaa cttctaccgg
    13141 gtctcccagg ggagcaacct tgcggatgta gacttcaaca ccctttgtga tgcccatgtc
    13201 cataatgcgg cgctttacag caccctcacc ggtcagtttt tttacccgga cggtctgtcc
    13261 gattttggcg tcttttaatg tagccattgt tgaattcctc ctcttatatc ataatttttg
    13321 cagccatttc tttggtaatg gcaatacgag tctctttgac attgacgatg agatttccgc
    13381 tgccggaatt caccaccgta agtgttccgc caaccacaaa gcccagatct tccaggtgct
    13441 ttttggtctc ggcacttccg ccaacctttt tgatggtaac ttcatcacca gggtttgcaa
    13501 acatcagcgg catcatacca gttttcctcc tttttccgaa tgcgatcgcc tgtttatgca
    13561 aaagcagaaa caagcaaaat ttgttagaaa aagctaacct attgtaaaaa cagaagagtt
    13621 agaacgctct aaccctcttg atagtataca acttctgacc
//
LOCUS       DAWENO010000045        13630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1875_length_13630_cov_12.083683, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000045 DAWENO010000000
VERSION     DAWENO010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13630
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_13630_cov_12.083683"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4608..4617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6875..6884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3402 a         3444 c         4369 g         2385 t
ORIGIN      
        1 agccatgccc acatgatcgg ggcatcggca ctgtacgatg agacacggga aaaatccgag
       61 agttccgtga tgacgctggg agaatccgac gctgtgcagc agcctggccg ccggaaacag
      121 cgtccgccgg agccggagat tccggaagag accgaacccg acccggcgat cgaccccatg
      181 ccggaggaag agctggaggg tctggcagga gagatcctgt ctctgctgca aagacagcgt
      241 gtgatgcaca tggatcagat cgcagcggag ctggaggttt ccatggagga actgaccaca
      301 acgctgacgg aactggaact atacgggaaa ctggaacgcc tgccgggaaa acaattccgg
      361 cggatgcgtt agtgcgcagg aaaacctgcg gaaacacgga ataaagcctg tgtgcaaagc
      421 tgttaggcag gctttatgca gcgtttccct gttttgaaga cgattttgcc ttgagagaga
      481 acgagagaga gttatgtcga atttagtgat cgtggagtcg ccggcaaagg cgaaaaccat
      541 tcaaaaatat ctgggcaagg gctatgatgt cattgcctct atgggtcaca tccgggatct
      601 gccgaagtcg aaactgggtg tggatgtaga aaacggcttt cagcctcagt atactgatat
      661 gaagggcaag gaagacgtta tcaaaaaact gaaagaacac gcaaaaaagt gcgaccatat
      721 ctatctggca actgacccgg atagagaggg agaagccatt tcctggcata tcgcacagat
      781 gctgcattta gatatggatg ccgatgaccg ggtggagttc aacgagatca cccggagcgg
      841 tatccaggct ggtatgagcc atccccacaa gatcgacctg gatctggtga atgcccaaca
      901 ggcacgccgt atcctggatc gtctggtagg ttataagctc agcccgtttt tgtggaaaaa
      961 ggtgcgccgt ggtctgtctg ccggacgtgt acagtcggta gctgtgcgcc tggtggtaga
     1021 ccgggaaaac gagatccgtg cgtttgtttc ccaggagtac tggtccattg atgcgaagtt
     1081 tacggcgccg ccgtcccgaa aggtgtttgc ggcaaagctg gtgaaggtgg acggcgaacg
     1141 ggtggacaag acggagatcc cggacaaggc tgccgcagat gctctgctgg aacggctgca
     1201 aaatgccagc tattccgtgc agaatgtgaa aaaacgggtg accaagcgcc accctgcacc
     1261 gccgtttatc acctcgaccc tgcagcagga tgcctcctat cgcctgggct ttcagtccaa
     1321 gcgcaccatg aaggtggcac aggaactgta cgaaggcgtg gatatcgacg gactgggtgc
     1381 agtcggtctg attacctata tgcgtaccga cagcctgcgt atctccgcag aggcgcagca
     1441 ggcagccgca gactatgtga ccggaaaata cggcgcagac tatctgccgc cggagccgag
     1501 agcatataag tccaagaaga acgcccagga tgcccacgag gcgatccgtc cgtctatgcc
     1561 ggacatgaca ccggagcggg tgaagtccag tctgactgcc gaccagtaca agctgtaccg
     1621 gctgatctgg gagcgcttta ttgcaagcca gatggcagat gcgctgatgg atacggtgtc
     1681 tgtggacatc gaggcagaca actgcatctt taaggcaacc ggctcgaccg tgaagtttga
     1741 cggttttacc gtgctgtacg acgatgccaa gggcgacaag agcgagaaca agaacctgcc
     1801 ggagctggaa aagggcatgg agatcaaggt gaagtctctg gaaggcaacc agcacttcac
     1861 ccaggcaccg ccacgctata cagaggcgac gctgatcaag gcgctggaag aaaacggcat
     1921 cggccgaccg agtacctatg cgccgaccat caccaccatc accacccacc tctacgtgga
     1981 gcgtgaggga aaacagctga aacccaccgt cctgggcgaa gtgaccacgg aactgatgaa
     2041 agagcatttc cccaacatcc tggatgtgaa gttcaccgca gagatggaaa acaacctgga
     2101 tgatgtggaa cgtggcaaga aaagctgggt ggatacgctg gaggacttct acggcgattn
     2161 nnnnnnnnng cgatttctca aagacgctgg agactgccga ggaaaaaatg gaaggcaagc
     2221 gtgtcaaagt gccggatgag gagacggata tcgtctgcga tcagtgcggc agaaatatgg
     2281 tcatcaagat cgggcgctac ggacgctttc tggcgtgtcc cggattcccg gagtgcaaga
     2341 acaccaagaa gatcgtccag gaaactgccg gttcctgtcc gctttgcaat aacaagatca
     2401 ttctgaaaaa gtccaagcgt ggcagaagtt tctacggatg cagcgcttat ccggattgca
     2461 acttcatgac ctggaatgtg ccgaccgagg aaaagtgtcc ccagtgcggc tgtaccctgt
     2521 tccagaaagg cggcaagtcc ggcacgctgg tatgcgaaaa gccgggctgc ggctattccc
     2581 gtccggtagg aggtgcgtaa atggcagacg taaccgtgat cggcggcgga ctggcaggct
     2641 gtgaggctgc ctggcagctg gcagaggctg gcttttccgt gcagctgctg gagatgaaac
     2701 cggtgcagta cacgccggcg caccactatg aggggttggc ggagctggtc tgttctaact
     2761 cgctgaaagc cgaccgcatc aattctgcgg cagggctgct caaggcggag atgacccgcc
     2821 tgggttcact gctgatgcag tgcgcccgga aatcggcagt ggctgccggc ggtgcgctgg
     2881 cggtggatcg gaaacagttt tccgacctcg ccaccgatgc catccggaat caccccaaca
     2941 tcaccctgga gaccgccgtg gtcgatcgta tcccggatac tccggtagtc gtggcgaccg
     3001 gaccgctcac agaaggcgca ctggcggcag acatcgaaaa gcgctgcggc acacggctca
     3061 gctttttcga tgcggctgcg cccatcgtca gctttgagag tctggacaag gagaaagtgt
     3121 tctttgcggc acgctacggc agaggggatg cagattatat caactgcccc atgaacaagg
     3181 aagagtacga gaacttctac gaggcgctga ttgccgcaga acgtgcgcct ctgcacgact
     3241 gcgacaagga tgcgttccgg gtctatgagg gctgtatgcc cattgaggta ctggcatccc
     3301 gtggcccgga taccatgcgg ttcggaccgc tgaaaccggt gggactgacc gatccacgca
     3361 cgggtcaccg tccgtgggcg gtggtacagc tgcgccggga aaaccaggag ggaaccatgt
     3421 acaatctggt gggatttcag acgaatctga aatttccgga gcagaaacgg gtgttctcca
     3481 tgattcccgg tctggaacac gccgagtttc accgctatgg cgtgatgcac cggaactcgt
     3541 tcctggacag cccacgcctg ctggatgcgg actatcattt ccgtggacag gacaccctgt
     3601 actttgccgg acagatgacc ggtgtggagg gttatatgga atcggcggca agtggcattc
     3661 tcgccggaaa gaacctggca cgccgcttgc agggcaaagc gccgctcatt ctgccacggg
     3721 agaccatgat gggcgcactg gcggcataca tcagcagcgc acccacggag aatttccagc
     3781 ctatgggtgc aaatatgggc attctgcccg acctgccgga gcgcatccgt ggaaagcagg
     3841 aaaagtacca ggcgtatgcc gaccgtggca tggcggcgct ggaacagtat ctgcgtgagg
     3901 cagaacaata atacaaggga gatcatacat cttgaatata ctaatcgacg cattcggcgg
     3961 tgacaacgca ccgctcgaag tgattcgtgg cggcgcacag gcggtgcagg aactgggcgt
     4021 aacggtgacg ctggtcggag acgagcagaa gatccgggac tgcgccgagg aaaacggtct
     4081 gccgctggac ggcatggaaa ttctccacgc cccggcagtg ttcgacatcc acgaagaacc
     4141 cacatcgctg ctgaaacagc acgccgacac ctccatggca gtgggaatgc aggcgctgca
     4201 tgacggcaaa ggcgatgcct ttgtctgtgc cggaagtacc ggcgcactgc tggttggcgc
     4261 aacctttctg gtaaagcgca tccccggcat caagcgtgct gcggtcgctc cggtgctgcc
     4321 cacagagacc acgccgtttc tgctcattga cggcggcgca aacaacgact gccgcccgga
     4381 aatgctggtg cagttcgcca ttatgggcag tgcctatatg cagaacgtca tgcaggtgga
     4441 aaagccccgt gtggcactgc tgaacgtggg cgcagaagag accaagggca gagaactgga
     4501 actggaatcc tacgtccagc tgtcccaggc gccggtgaac ttcatcggca acattgaggc
     4561 gagagagctg cctgccggcg cagcggatgt ggtcgtcaca gacggctnnn nnnnnnngtg
     4621 ggcggcgctg tgatgctggg cgtacagaaa ccggtcatca aggcgcacgg cagctctgat
     4681 ggcacggcat ttttcaacgc catccgccag gcgagagact gcgttgccgg aaatgtggta
     4741 cagaccattg cagaaaatac cggaagaccg gtaaagaaat agagggaaag caatcatgga
     4801 acatacagag tatgtagatt tgggacggtt tgaaacctat atccactatc atttcaagga
     4861 caagcacctg ctgttggagg cgctgtccca ttcatcttat gccaacgaaa agcgcaagac
     4921 acgcaagtgc aacgagcgcc tggaattcct gggagacagc gtgctgtcca tcgtggtttc
     4981 cgagttcctg tttcacaagt atccggaact gccggagggc gagctgacca agctccgggc
     5041 atctatggtc tgtgaaaagg cgctgcacgt tttcgccaag gagatccacc tgggcgaatt
     5101 tttgctgctg ggcaagggcg aagagcatac cggcggcaga ttccgttcgt ccattctggc
     5161 agatgccttt gaggcagtga tcgctgccat tttcctggac ggcggcatgg atgcggcaaa
     5221 gcggcatatc ctgcggttta tcccggatga tccggaagag gagaaaaagc agccctccgg
     5281 tttccacgat tacaagaccc agctccagga agtggtacag aagaacccgg aggaaaaggt
     5341 ggaatatgtg ctggtcagcg agtacggccc ggatcacgac aaggcgttcg aagtcgaagt
     5401 ctgcctgaat tccaacgtga tcggcaaggg catgggacac agcaaaaagg cggcagaaca
     5461 gcaggcagcc aaggaggctc tgtcgctcat gggatatggc aaagcataag actcttgcct
     5521 ttttcattcc gcaccgtggc tgtccgcatc agtgcagttt ctgtgaccag cgcaccatca
     5581 gcggcaccgt ccatgcgccg gagccggaag agatcacaca gacctgccgg gaaatgctgg
     5641 agcgcagcgg aacgctggag tcggcggaga tcgccttttt cggcggcagt tttaccgcag
     5701 tagagccggc gtatcagatc gccctgttgg aggcggcaca gccgtttctg ggaacaggaa
     5761 agttttccgg catccgcatc tccacccgtc cggatgccgt tgatgcagcg gttctggaac
     5821 ggctgaaagc ctatcatgtg acggcagtgg aactgggcgc acagagtatg tgcgaccgtg
     5881 tgctgcggct gaatgcccgt ggacacgatg cacaggcagt ccggacggca agtgccatgc
     5941 tgcggcaggc aggcttttcc ctgggcttgc agcagatggt ggggctgtac ggcagtacat
     6001 tgcaggacga atacgacacc ctggaacagc ttttggcgtg ccggccggag accctgcgga
     6061 tctatcccac agtggtactc gccggaacaa agctggcgga gtactgtcag cccggcgtgt
     6121 atccggcgct gtcacaggaa gaggtgctgg actactgcgc cgatgccctg tgccgctgtc
     6181 atgaggctgg ggtgcgtgtg atccggctgg gactgcacga cacgccgtcg ctccgggcag
     6241 ggatgctcgc cggatatttt cacccggcat acggagaact ggtggaatcc cgtctgtatc
     6301 tgcggcagct gcaaaaggcg gcggcagact gtggaaaacc ggaactcttt gtggaaacag
     6361 caccccgtac catgagcaag gtgctgggac agcatggttg caaccggaaa atgctggcag
     6421 agcagggcgt aacgctccgt gtgcaggaga atgcggcggt gtcgccggga acacttcttg
     6481 caaatggaaa atgctgtggg gtgtatggca atgtctcgga gaaatgaggg aatatatgac
     6541 cggaaagaaa tatagggtcg tggcgctcat ccttgcggcg gtcattctgg cggcaggcgg
     6601 tgtgctgtgt tacaggcatt ttcatcagac ggcgcaacct gtcaccgcag aagcatccgc
     6661 agagactgcg gcggcggaga cggtgatctt ccgacagaag gatgaccgct ggaaagacga
     6721 tgcactgggg gattctgcct atcacatggc ggattccggc tgtctgacct gttgtgtggc
     6781 ggcggcgctg caaatgcagc agatctctgt ggacggtttg ccggaagatg ccgatgccgg
     6841 agcagtgaac cagttctttt cggaacaggg cgtgnnnnnn nnnngggcgt gtatgacgga
     6901 cagggaaatt tgcagtggga cgtactggaa caagtgacag gcgttccggt gctgcggcag
     6961 gatgctgcgg aactgccgga gggcgcactg gacgagtatc tggcggacgg ctgttttccc
     7021 attgtccggg tgaagatgcc gaaaagcggc agcacccact atgtactgct gacaggcagc
     7081 gaggacggga cgtatcagtg catggatcct ttgcagaaaa aggaacagac cgtgccgctg
     7141 tctgatttcc acaatcagat ctatgccgtg cgtgtgctgc aatcgccaaa ataaatgttt
     7201 cgaaagaggt gagacatcga aatgtatctg caatccctgg aattacaggg ctttaagtcc
     7261 tttcccgata agatcaagct gcactttgac aagggtctga ccgccgtagt gggacccaac
     7321 ggcagcggca agagcaacat cggcgatgcg gtgcgctggg tgctggggga acagtctacc
     7381 aagacgctcc gtggcaacaa gatggaggac gttattttct ccggcacaaa gacccggaaa
     7441 gcaatgggct ttgcggcagt tacgctggag atcaacaacg agaccggcga actgggagat
     7501 gcctatggtg ctgtagtcag tgttacccga aagctgtacc gcagtggtga cagtgagtac
     7561 cgcatcaacg gcaaaaatgt ccgtctgaag gatgtgacgg aactctttat ggataccggt
     7621 atgggacgtg acggctatgc catcatcgga cagggacgta tcgctgagat cgtcagcgcc
     7681 aaaagcaccg accgccggga tatttttgag gaggcggcag gtgtctccaa gttccgctat
     7741 aaaaagcagg aggcgcagcg caagctggat gctgcacagg aaaacctggt gcgtctccag
     7801 gatatcgtca tggaactgga atctcgtgtg gaaccgctcc ggcagcaggc ggaaaaggca
     7861 aagcagtatg tggagcttgc cggggaacag aaacagctgg aagtctctct ctgggtgcga
     7921 cggctgacgg cactccggga aaagcttacc gggctgtcgg acggctatct ccaggcacag
     7981 gcggaatatc agaacgccga acgggagata cagcggacag atgcccaggt gcaggaaggc
     8041 tatcgccgga tgcaggaaag cacgctgaaa atggaagaga tccgtcagaa gatccagcag
     8101 gcggaagaag aggcggcaca gctccgggcg gcggtggcag tgtgcgagaa cgacattctc
     8161 cacagccgtc agatgctgga aagtctgcac agtcagaaaa cggagacggc ggaacagtcc
     8221 aggagtatgg agagccggat ggcatccctg tcggaacaga ccgtgcagct gcggcagaaa
     8281 cgtgagtcgc tccaggtgac gtcggaacag gcggaacagg aatgggagag actgaaacag
     8341 caggcggcac ggcgtgggca ggaatacgag caggcgaacg gcatcctgca aaagctgctg
     8401 gtgcggcaga gcgaactgcg tgtcatgcag cagactgccg ccggacagca ggaagaactg
     8461 aaagcccagc tgacggaaca gggaatgcgg cagagcgctt tgcagcaggc gtgttccagt
     8521 cagaatatgg tctacgatgc cgccagagaa aaggcggagc aggcgaaggc acttttcgag
     8581 caggtgcaga accagcgtgc cggttttcag aaacgctacg cccatgcggt gcaggcgctg
     8641 gaagagggca gaaagaaccg ggaccaggcg gcgtttgcac tccgggaaaa gcgccagcgc
     8701 cagaaactgc tccaggatat ggagcggaac atggagggct ttggcggcag cgtcaaggca
     8761 gttcttcggg cggacggcgg aaagccccgt ggggtgttcg gcacggtggc acagcttatc
     8821 accaccgacc agaaatacgg cgtggcgctg gaaaccgcac tgggcggcgc catgcagaat
     8881 ctcattgtgg aaaacgaaaa cgctgcgaaa cagtggattc gctttttgca gcaacagcgt
     8941 gccggacgtg ctacattcct gccgctgact tctgtccgag gcagaacgct gaacgagacc
     9001 gggctggaac gccacgaggg atttatcgcc atggcgagcg aactggtgcg ctatgaccag
     9061 cgcttttccg gcgttgtcca gtttttgctg gggcgtgtgg ctgtggcaga ggatctggac
     9121 agcgcaacgg cactggcaaa ggcgtatcag taccggttcc gcattgtgac gctggacgga
     9181 caggtcatca atgcaggcgg ttcgttcacc ggcggttcgg caatgcgttc cggcggtatg
     9241 ctgacacgcc gtgcggaact gacggcgctt tccggtgagg taaaggcgct ggaacagcag
     9301 gagcaggcgg cggcagcggc ttttcagaag gcgcaggcac agtcggcgca gctggaacag
     9361 cagatggaag aactccagca gcggtgccgc cgggcagaat cggaacaggt cgccgcagag
     9421 gcacagctgg aaaaggaaca gtatctgcga aagcagaaca cagagcgtct ggaagaggcg
     9481 gcatcgcagc aggcagtact gcagcagcgg atgcaggaga ctgcggataa atacaccgcc
     9541 gctgccgaag aggcagaaga ggtggaaaaa gcgatacaaa agggacagga gatgctgtcc
     9601 caggaggcag accagcgcag cgccctggaa caggagcagg ctcgtctggc ggaacagcgc
     9661 tcacagctga agatcagcat gatggagctg gaaaaggata tggatacctt gtccagagag
     9721 atccagcagc agagcgacca gcaggattcc atggtagaaa agctggcgca gatcgacagg
     9781 gatatttcca cccaggaaga actcatccgg cagaaacagg acgagatcac ccgtggaaac
     9841 aagactctgg aacagatgaa aacaagccgc actgacggtc aggcggagat ccggcactgg
     9901 cagcaggtgc atgatgccca ggatcagcag atccgggaga tgcagagcgg actccgggag
     9961 accaacgccg tccgggaaaa gttctccggc gagatgagcc ggctggaaga gcggaaaatc
    10021 gctgtccaga gcgactatga cggcgtaata cgccaactgc tggaacagta tgaactgtct
    10081 ctgtcagagg cgcaggagca ggcgcagccc ctggaagatc tccaggcggc gcagcgggaa
    10141 ctgcaaagtc tgaaaagccg catccgtgga ttgggtacgg tcaatgtggc ggcaatcgcc
    10201 gagtacgaag aggtctccgc acggtatcag ttcctgtcgg aacagatgca ggatgcagag
    10261 acggcaaagc gggaactgga agacctcatc acctcgctga ccgaggagat gcagcgcatt
    10321 ttttcggaga gcttttccat tatcaaccgg aatttccagg agatcttccg ggatctgttc
    10381 ggcggcggcg aggcacgcct ggaactcacc gacccggaaa atgtactgga atcgggcatc
    10441 gagatcaagg tcgcaccgcc gggaaaggtc atcaagaatc tgatctctct gtccggcggc
    10501 gaacagtcgt ttgtggcaat cgctatctat tttgccattc tgcggctgcg gccgtctccg
    10561 ttctgtattc tggacgagat tgatgcggcg ctggatgagg gcaatgtccg gcgctatgcc
    10621 cagtatttgc agaattttac cgataccaca cagttcatcc tggtgaccca ccgccggagc
    10681 gccatggaag aggcaagcgt cctctacggc gtgaccatgc aggaggacgg catttcccgt
    10741 ctgctgcgga tggaacagga agaacttcaa tagaaaggac aggtgtacca tgggcttatt
    10801 tggcaagaag aacaaaaaca aagaaaaggg cgtaccgttc tggaaacgca ataaacaaaa
    10861 gcaggaagaa gaactggtac aggaagaact gacggagcag gaaagcccgg cggaagaatc
    10921 tgacggggca ttcgctgggg aagaagagga catcgccgcc gctgccatcc gggaacagga
    10981 agcgcaggag caggacgagg tggagaaagc ccgtcttgcg gcacaggcag tggtggaaga
    11041 atcagaacag caccaggagg caattgccct ggcacggcag gcggcattgg aggctgccgg
    11101 gattctgaaa agcgaagagt ccaagccgga agaagaaact gaaccggaag aagaatccga
    11161 accggaagag gaaactgaac cggaagaaga atccgaacca gaagaggaaa ctgagccgga
    11221 agaagaggct gaaccggaag aagaaactga accggaggaa gaaaccgaac cggaagaaga
    11281 gactgagccg gaggaagaga ctgaaccgga agaagagact gagccggaag aagaaactga
    11341 accggaggaa gagaccgaac cggaagaaga aactgaaccg gaagaggaga ctgaaccgga
    11401 agaagaatcc gaactggaag aggaaactga accggaagag gaaaccgagc cggaagaaca
    11461 gccggagaaa aagggctttt tcgccaagat ccgtgacgga ctgcgcaaga caaaggatgc
    11521 catgatgtcc cggatgcagc aggtgttgaa cgggttcacc aagatcgacg acgacctgtt
    11581 tgacgaattg gaagagacca tgattaccag cgacctggga ccggagacct cggtggagat
    11641 ctgcgagacc ctgcgcaagc gtgtgaaaga gcgtggtgta aaggatcccc aggagatcat
    11701 gggcatgatc caggagatca ttgcagagat gctgggcgag gatcagacgc tggaatatcc
    11761 tacaaagccc accattgtca tggtgatcgg cgtcaacggc gcaggcaaga ccaccaccat
    11821 cggcaagctg tgccatcagc tgaaaggcga gggcaaaaag gtgctggtcg ctgcggcaga
    11881 tacgttccgt gcggctgcca ttgaccagct ggaggtctgg acacagcgtg ccggcgtgga
    11941 tattgtcaag cacgccgaag gctctgaccc ggcatcggtg gtctatgacg ccattgatgc
    12001 ggcaaaggca agaggctgtg atgtgctgat ctgcgacacc gcaggacggc tgcataataa
    12061 gaagaatctg atgcaggaac tggcaaagat caaccgcatc attgagacga gagcagaggg
    12121 atgccatcgg gaaattctgc tggtgctgga cgcaaccacc ggacagaacg ccgtgaacca
    12181 ggcgagactg ttccaggaag tggcagatat ttccggcatc gtgctgacaa agctggatgg
    12241 tactgcaaag ggcggtatca tcgtatccat tcgcaatgag ctgggcattc cggtaaagct
    12301 ggtgggtgtg ggcgaaaaga tcgacgatct ccagccgttc cacgcacagg actttgtccg
    12361 tgccctgttt gaaccgacgg aggaatgacc atggaaaaaa tcattattgc atccaacaat
    12421 gccggaaagc tccgggagtt ccgggcaatc ctggaaccat tcgggctgga agttgtctct
    12481 cagcgtgagg caggcttttg ccaggacgtg gaagagaccg gcaccacatt tgcagaaaat
    12541 gcggcgataa aggcaaaggc agtctatgcg gcaatgcact gtccggtcat cgccgatgac
    12601 agcggtctga tggtggacgc tctggacgga gcgccgggcg tgtattccca ccggttcgcc
    12661 ggagagggcg caacggatgc agaccgcaac gccaagctgc tgcacaaact agacggcatc
    12721 cccgcagaaa agcgcacggc acggtttgtc tgctcgctct gctatctgga tgccaacggc
    12781 acggaaaagg tgttttccgg tgtctgtgag ggcgtgatcg gcacagcacc cagaggagaa
    12841 aacgggttcg gctatgatcc gctgttctat gtggacggaa agtccatggc ggaaatgaca
    12901 gaggcggaaa agaaccggat cagccaccgg ggaagagcgc tggaacagct gaaagcattt
    12961 tttgaagaga aataaaagga gaactttttt atgttgacaa gcaaacaacg ggcgaccctg
    13021 cgtggcatcg ccagcacata cgaaaccatt ttccaggtgg gaaaaggcgg catcagcgat
    13081 acgctggtac agcaggtcag cgatgccctg cgcaagcggg aactgatcaa aatgcacgta
    13141 ctggacaact gccctctgga tgccagagag gcggcgcagg agctggcgga aaagacagag
    13201 tcagaggtgg tacaggtcat cggcaatcgt ctcgtcctgt tcaagcgcaa tcccaaagag
    13261 ccggtggtaa agttctgatg cagcgcatcg gactgttcgg cggcagcttt aaccccatcc
    13321 acaacgggca tcttcacctg gcgaaagcag caaaggaagg gctgggactg gacaaggtcg
    13381 tgctgatccc ggcaaatgtc tcgccgttca aacaggatgc gccgggcatg gcgtccgggg
    13441 cggaccgtct ggcgatgtgc cggcttgccg cagaaacgct gtccttttgt gaggtggaca
    13501 gctgcgagct ggaacgctcc ggggtcagct atacggtgga taccgtccgg ctgttccggg
    13561 agcggtatcc ggaggcggaa ctggtgctgc tggtgggcag cgatatgttt ctctcattca
    13621 cccggtggca
//
LOCUS       DAWENO010000046        13155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1985_length_13155_cov_10.005954, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000046 DAWENO010000000
VERSION     DAWENO010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_13155_cov_10.005954"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3271..3280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8995..9004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3126 a         3157 c         3770 g         3082 t
ORIGIN      
        1 gcggcaatac cagagaggag atgctatgat acagaaattt acactggaat cccaccacga
       61 ccgcctgttg ctccagggac tgctggtgct gccggagcag aaaccgccac gggcggtttt
      121 ccaggtcgct catgggatgt gcgaacataa ggaacgctat cttccgtttt tgcaggagat
      181 ggcagatcac ggctatgtgt gtgtgataca cgatcaccgt ggccacggcg gcagtgtgcg
      241 tcagacggag gatctgggat acttttatcc ggacggcggt ccggcgctgg tgtccgatct
      301 gtaccaggtg acacgctgga tacgggaaca gtacccggaa ctgccgctgt acctgttcgg
      361 acacagcatg ggttccctgg cagtacgctg ttatctgaaa cgctatccgg atgtgccgga
      421 cggcgtgttt gtctgcggct ctcccagcgc tgctttcggg ctggaactgg gagaacgtgt
      481 ccgggcagtg ctggcaaaga aaaagggcgc atcctaccag agcaagcggc tggcggatgc
      541 tgtcttttcc ggattccaga aaccgttccg ggatgagggc agggacaatg cctggatctg
      601 cagcgatccg gctgtggtag atgcgtacaa tgcagatccg ctctgcacgt tctgctttac
      661 gctgaacggc tatgaggcac tgttgtacct gctccgcacg gcgtacagcg aaacggaatg
      721 gcgtgtgaca aagccggaac tgcccatccg gtttctttcc ggggcagatg acccgtgccg
      781 gggaaatgat gaaaagtttg cccaggcggc acagctgctg cggcaggtgg gctatcagaa
      841 cgtgtcgtcc cggctgtttc cgggaatgcg gcacgagata ttgaacgaaa cagaaaaatc
      901 gctggtgtac caggacgtgc tggaaacgct ggatgcgtgg cagtccaaat aggaggaatc
      961 gaaaatgaca ataccaaagg aaatacagga aaaccgcatc gcctgggcaa aatcggatgt
     1021 gcagcgtgac aaggggctga ccatgccgga ggacgtggaa cagttctgcc agatcgccta
     1081 tgcagaggat ggactggcgg cgcatacgct gaatgtgtat cgtccgaaga atgcagggga
     1141 cacgctgccg ttgctcatca gcattcacgg cggcggctgg ttttacgggg acaaggaact
     1201 ttaccgtttt tattgtgcgc atatggcgca gctgggattt gttgtggtag actgcgacta
     1261 tcgccttgcg ccggagtatt cctatccggc ggcgatcgag gatgtgtgcc gggtggtgcg
     1321 ctggtcgctg gaaaaccggg aaaagttcac ccggaacaag cactggttta tggtgggcga
     1381 ctctgccggg gcgcagctgg tgagccagta ctgcattctg gcaggcaatc cggcataccg
     1441 ggaacagctg gacttctgca cctatgacca gctgccggac ggcgttgcgc tgaactgcgg
     1501 catttatgat atgtccatgg ataatgacca catcggaaac tacacccacg aaacgccggc
     1561 ggcacagaaa aaactgctgg aacaggtgct ggattacatg aacgacagtt tcccggcggc
     1621 atatgtgatg accagcgtca acgatggtct gcggccgcac acagcgccaa tgaagaaaaa
     1681 gctggaaacg ctgggtgtac cgtttgtcta tgcggaatac ggcgagggtg tcccgtcgga
     1741 cagccacgtg ttccatctga acctgtacag cgaaaacggc agacgctgca accaggcaga
     1801 ggcggatttc ctgcatggac tggtaaagga ataacagaga caagaaaaca ctgctcttga
     1861 caatcttttg aaaaaacatt ataatataac atggcataat ttgattcgaa tgaaactctg
     1921 ggaggaagag agaacaatat gatggaacat atccgcaatt tttgtattat cgctcatatt
     1981 gaccacggaa aatcaactct ggctgaccgt atcctggaac agacctatac ggttgccaag
     2041 cgtgatatgg aagaccagat cctggacaat atggatattg aacgggaacg tggcatcacc
     2101 attaaggctc gtgcagttcg gctgaattat cgtgccaagg acggcgagac ctatgtgttc
     2161 aatctgatcg acacgccggg acacgttgac tttaactatg aggtctcccg cagccttgct
     2221 gcctgtgagg gtgcgattct ggtggtagac gcctcccagg gaatcgaggc gcagacgctg
     2281 gcaaatacat accttgccat tgagcatgac ctggaggtcg tgccggtagt caacaagatc
     2341 gacctgccgg cggctgaccc ggaccgtgtc tgcgaggaga tcgaaaatgt gatcggcatt
     2401 ccggcagagg atgcaccccg tatctctgca aaggcaggca tcaacattga gtcggtcatg
     2461 gagaagatcg tgacggacat ccctgcacca aagggcgacc cggatgcacc gctccaggcg
     2521 ctgatcttcg acagctacta tgacgcatac aagggcgttg tcatctatat ccgggtaaaa
     2581 cagggtacgg taaaggtcgg cgataccatt aagctgatgg cgaccggctc ggaatttacc
     2641 atcgtggagg ctggctatat gaccaccaac acgctggtga atgagggcgt gctgcgtgcc
     2701 ggcgaagtcg gctatctttc cgcatccatc aagagcatcg gagacacccg tgtgggcgat
     2761 acggttacac acgctgaccg tccctgtgca gagccgctgc cgggttaccg caaggtcaac
     2821 cctatggtat tctccggtat ctatccggca gacggcgcaa agtacggtga tctccgggat
     2881 gcgctggaaa aattgcagct gaacgatgcg tcccttacct ttgaaccgga gacctccatt
     2941 gcgctgggct ttggtttccg ctgcggattc ctgggactgc tccacatgga gatcatccag
     3001 gagcgtctgg aacgggagta caacctggat ctgattacca cagcgccgtc ggttatttac
     3061 cgtgtcacac tgacaaacgg cgaggtgcag tatatcgaca accccaccaa ttatcccgac
     3121 ccggcagtaa ttgccaaggc ggaagagccg atggtcaagg cggaaattct gacaccggca
     3181 gacttcgtgg gaaatgttat ggaactctgt cagaaccgcc gtggcgtgtt tgtgaatatg
     3241 cagtacctgg acgatacccg tgtatcgctc nnnnnnnnnn ctgccgctga atgagatcgt
     3301 ttacgatttc ttcgatgtgc tgaaatcccg tacccgtggc tatgcgtcct tcgactatga
     3361 gctgagcgac tacatggaat ccaagctggt aaagctggat gtactgctga acggcgagat
     3421 cgtcgatgcg ctgtcgttta tcatccatac cgacaaagcg tatgagcgtg ccagaaagat
     3481 cgtggaaaag ctgaaggaga acatcccacg gcagctgttc gaggtgccgg tacaggctgc
     3541 catcggcggc aaggtcattg ccagagagac catcaaggca atgcgcaagg acgttctggc
     3601 gaaatgctac ggcggtgaca tcacccgtaa gaaaaagctg ctggaaaagc agaaagaggg
     3661 caaaaagcgg atgcgtcagc tcggttcagt agaagtgccg caggaggcat ttatgagcgt
     3721 gctgaaactg gatagtgatg attaaaactt aaaacaaaac cataaagcaa actggctgcc
     3781 gcaccatata aaggtgcagc agccagtttt cgtttctata aagtttgcct tattcctttt
     3841 tctgttccag attctgatag ggcaggggct tggaaaaata atagccctgg gtgtactgga
     3901 tgttggtgga ttccaccgtc tgctgttcgc cgctgttctc tacgtgttcg gcaatcagtt
     3961 cggcgttaat gcctttggcg atatcggaga tcgcctgtag gcaaagctgt ttcatcgggt
     4021 ccttatcgca gttgatgata aggtcgccgt cgatcttgat gaagtccgga ttgatttgca
     4081 ggattgcagt gaagttggag aatcctgcgc cgaaatcgtc aatggcgatc ttgacgccga
     4141 acttgcggat ctcgttcagg aagtctacca tttcattgaa atcccggatc ttttccgatt
     4201 ccagaatctc aaaggtgatt cgctcacacg ccccgggcgg cagatcgctg agcaggtcgt
     4261 acagcaggga acgacttgca gaggaactga tatcgtaggc ggagaggttg aggaaaacgg
     4321 tttcctttcg atcccggaac agttccagca ccttttgtat catcagctgg gacagctgca
     4381 aatacaggtg atagtccttg gcgatttcca ggaactgtcc cggcgcatag atattgtgat
     4441 ccttgtccga cagccgcatg agcgcctcat acttggtgat ctcctgggtg cggttgtccc
     4501 ggatgggctg gaagtagggg acgatacggt catagagaat ggcatcgctg atgatgttgg
     4561 tcatgcgcag cttttcgtcc atctgtttca tggtctcggc gttgtccttg ttgtacagga
     4621 aaaaacgtgc gttgtggtca gccacggcgg acatacaaag ctgtaccttt tccagcaggt
     4681 tggtttcgcc gatgaccaca tggaagatgt tgacagctgt aatgttcagt tccttgcaga
     4741 agtaatagcc gcattcgtgg aatgccagct ctgcctgcgc caggaacttg tcgtccggat
     4801 aatccggtcc ggcggcaaag aagaaagacc aggtgtcgtt gaagtaaaaa gacagctctg
     4861 tatcatgcag tgtgcggatc atctgctgaa tgttttcccg catcagctga ttccaaactt
     4921 ttgtaccacg gcggccggaa atcagaattc ccttttcaat ggaaatcata cagattttgt
     4981 cgaaccgctg ttttcggtca tcaaatttga atttcacgag attatccagc tccatttctt
     5041 tgtcgaagaa catattgctt tgcagaagtg cctcctgcaa ctgaatctgc tttgcgatcg
     5101 agggcagatc tgctttctga cgcatatgtt ccagatattg attgatgttg tgccagtctg
     5161 catccagcgt ttgcagatcc tccagggcag tccagtcaat gggaagatat gcgtggttgg
     5221 atgcggagag tccgaacagc gtgttcgcac ccacatatac ctggttgtgc tgtgccgctc
     5281 tgctgatttc tccgtagagg aatgcgccgc agatgcggca gctgcgaaag acacgcagtt
     5341 cccattctgt gcagttttgc atggcaatgc ggtgaagtgt tccggggtaa gtaaatatca
     5401 cttcacaggg cgtggtttcc agctcgctgc attgtttctg gagttctgct gctgcggtca
     5461 tggggctgag gtatgccatt tggaattccg tccccggagg cagccagttg gaataggtct
     5521 cgatgcggtc acgtggttcg cagtaatgga tgaactgtcc cggattgccc ggcttttgca
     5581 gccggagcgg aaagtggagc agcagatctt cactcagccc gtcgtctttg gcatggcgca
     5641 gttcccggag catcttctgg aaccactgta cagccggctg ctgctcgatc tctgcaatgt
     5701 aatcgtgatt ggacttggta atgcggtgtg tgccgccgaa tgtctccagt ccggtgacag
     5761 cgccgctcca ggtgcggagc tgttcattgt tcaaaatcgc cagcagagcc gtgttgtgat
     5821 aaatgccgtt ttcgtcaaag gcaaatgtgc cgtataagtt attggtaatc atgccgccgg
     5881 cagccggaat atctgcgccg gtctcttcca gacggtcgag atatggctgc atctgcatat
     5941 actgatcggt gaagaatgca aacatcgcct tgctgtccgg cgtgagcagt ccggcagtct
     6001 cttctgccag cgctccgggc gtcttgtcgg caaaggaaag aggctgtacc tttacttgtg
     6061 tatggagcag ccgggtgaag atcagcaggc agccgtcctc atatatgctg ccgtgataga
     6121 tgaccgcaga tgcacttgtg ccgagaattt ttgcatccgg caaaagttcg gatacgtgct
     6181 tggcaattgc aacagcacct tccggcgtgt ggcagctggt gtggatcatg accagccacg
     6241 aactgtcctc ccggcgtgca ggaaggcagt attcctggat gaactgttcc agttccgtgc
     6301 ggttctggta taagagcgca gtggatgcga tattgctgtt tctcatggtg tcaccacctt
     6361 tattttcgtg cctttcggcg ctggattttg tcttcgtaca ttcggtcgtc tgcgaccttt
     6421 atcatttgat ccaggtcaac gttcttatcc ataggctgga tgcagaaacc gatgctggat
     6481 tctacctggt acggcttgtg agaaatgctg ttgtagcggt gtaggtattc ccggaaccgg
     6541 aatcggaaac tgtcgaaata gcagctgtcc gagtcggcaa ttacgccggc aacggtaaat
     6601 tcatcgccgc cgaaccggga acagacttca ccttgtatcg cactagagac cagcgctttt
     6661 cccacggtcg taatggcgtt gtctccctcg gtatgcccga aggtatcgtt gatatgcttc
     6721 agtccgtcca gatccgcaga aatgataaca atgctcatat tttttccgct gctctctttc
     6781 agctgctggt ggaactggcg atagaaacca cgccggttca aaagccctgt catgtggtcg
     6841 tggacgtaga gcttttccag ttcgctgttt gcgcttttca gctgcatatt gatggatttg
     6901 gtgtgcacct gggagtggaa gatgcccaga gacgaattca tggcgttcat gaaactgttg
     6961 atcttttcgt aggaatcatg agaaatatcc gtctggaatg cacagtagcc cattaccaga
     7021 tccagaaagt gcagggggca gaagatgacc ggatcttccc gccggagcat ggtggacaga
     7081 tcggggatca gctgttccag agatatcaca caggcatcct ggggcgccca gaagtagcgc
     7141 tgatacataa cgttgacctg ttcactgaac ggctcgccgt ttcggtggcg ctcgccaaaa
     7201 tccggcgcac ggaagatatc gtcgttcagt gcgaacacca tgctctcgaa gataaacttg
     7261 tcctggatga tctccggcag ttctgcaaga gaagtcatgc tggtgacagc ggactgtacc
     7321 tggcaggtca gttcctggcg ctcgttggca cgcttgagct ggtctgcgat atcctgcatg
     7381 gcgttgttga tgttcagcgg tgcaggcttt tcacagccgc aggactggga gtagaggata
     7441 tggaagtcta tgatgcgtgt cggctcgacc ggcagtccct ggtgcagacg aatggcagcc
     7501 gcaatgacct ctttgcccat ggtgtcgtag tcctgggtgc aggtggtgat gtgcggtaca
     7561 tgatagtttg cctcagagat gccgtcgaat ccggtgacga tgcagtcctc cgggatacga
     7621 tatccgtgat tttgcaggaa ctggctgcaa acgatcgcca tggtgtcatt ggcgcagatg
     7681 atggcgtccg gaatggcaca ctgttcctcg atgaaccatt gttccagggt ttctctcgtc
     7741 ggcttttccc agaagtcgcc gtagccgatg cggtagttgt ccggcgtaat gttgttttcc
     7801 gccatgacct tttcaaaggc gtggatgcgc tcgtcagaaa aggtgttgtc tctcacacca
     7861 gccatcataa agagccgctt ggcatggtgc ttctgcacaa cgtgctgaca gagcgtttca
     7921 aaagagttag aatagtcgaa ccggaagttg acacagcctt ccagaaactt atcaattgag
     7981 ataacgggga cattgtggct gcggcagctg tcgatgatgc tttgcagcac gctgtcccgt
     8041 ttgatggtgt ccgggaggac gatcatggca tccagcatat gatattggat catctggaaa
     8101 atggacatct caccgtcatc cgtcggtgtg agcggaagat agaagtctgt cttggaatgg
     8161 aaaatgaaaa ggcgatagcc ctgctgcacg gcatatttat tcaaggcacg gacgcattgc
     8221 aggcggtatt cctccggcag catagagaga catacgccga tgatgcgggg tgtgtttgtc
     8281 atcgtgtgga ccttctttct gttttgcaat ttgtgaaaaa ataccagttg agtatcttgc
     8341 agtaaattcc gaaactgtaa gaaatgcaca atttatgata tgttctatta taccatattt
     8401 ttttcgcctt gtgaagtatt cgaattgcac aaatttcatg aataaaaaat gtggattctg
     8461 tttgatgcaa aaacggattt tttcagaatg tgcagacggc acaaaagcat agcgtttcag
     8521 tatgtaaata ctttgtgaaa atcttgacaa acaaaccacg ctgtgctata ctagaataag
     8581 tgtatctatt ttttatagca ttctgacttt tcccggaaaa acctggtttt tcggagcata
     8641 tcaaacggct ggagcggcat ttccggccgg aaaggaatta agatcatgaa aatacgtgag
     8701 attcaagagg agatcctgcg gctgaaaaaa gaacgtgatt tctgtatcct ggcacatagt
     8761 tatcagacca gtgatattct ggaaattgct gattttacgg gggattccta tgcgctgagt
     8821 gtaaaggcaa agaatacgcc gcagaagaat gtgctgatgt gcggcgtgcg ctttatggcg
     8881 gagaccgtaa agatcctttc tccggaaaag aacgtatatc tttcgaaccc cactgccggc
     8941 tgtcctatgg cggagcagat ggacaaggag atgatccgtg cggtgaagat gcagnnnnnn
     9001 nnnnagtatc cggatcatac cgtggtcgca tacatcaata caaccgcaga gctgaaaacc
     9061 atctgcgatg tctgtgtgac ctcctcttcg gcggtgaaga tcgtctctca gctgagcaat
     9121 gacaagattc tgtttatccc ggactgcaac ctgggcgctt atgtgcagca gcagctcccg
     9181 gacaaaaagc tgaaactgct ccagggcggc tgccccatcc acgcatctgt atcggcgaag
     9241 gatgtccgta ttgccaagga ggcgcacccg gatgcgctgc tgctggtaca tccggagtgc
     9301 cgtccggcag tctgtgagca ggcggattat atcggttcca cttccggcat cgtcaactat
     9361 gccatggagt cggatgcaaa ggaatttatc atcggcacag agatcagcat tgcggagaca
     9421 ctccagaatg cctgcccgga aaagcggttc tatccgctgg caaagaaact gatctgcgag
     9481 aatatgaagt ccaccacgct gatggacatt tatcatattc tgaaagacgg tgagggtgcg
     9541 gagatccagc tggatgccaa gacgatcacc aaggcccgct gctgtatcga ccgcatgatc
     9601 gcattgggag gctgagcatg agagacctgc ccaaagacct gcactggctg ctgttcgacc
     9661 tggacggaac gctgaccgat tctgcgccgg gcattctgcg ctgtgtgcag tacgccctgg
     9721 aacaatgcgg cacgccggaa ccggacacgg acaagctgcg cccgtttatc ggcccgccgc
     9781 tgctggattc ctttcagcag ttctgtggaa tgtccccgga gcgtgcggcg tatgcggtgg
     9841 cgcagtatcg ggagcgcttt tccaagatcg gaatgttcga gaacagcgtc tatcccggaa
     9901 taccgcagct gctggaacgg ctgacggcac acggctatgt gctggcggtc gccacctcaa
     9961 agccggaggt gtacacccgg cagatcatgg aacactttgc actgacgaag tactttactg
    10021 taattgccgg cagtgacatt cacaaagagg gcgagacaaa ggcggatgtt atgcgtctgg
    10081 cgctgcgccg cctggggctg ccggaacagc agccgtccca cacggtgatg attggcgacc
    10141 ggaaacatga tgtgctgggg gcaaaggaat gcggcgtgcc gtgcatcggt gtccggtatg
    10201 gctacgctgc gccgggcgag ctggaacagt acggcgcaga atgggtcaca gatacgccgg
    10261 aaactctggc ggaactgctc ctggaatgca caatctgatg tttgctttat acggcagaac
    10321 acatggaaaa tatttgaaaa gcactgcaga aaatgcagtg cttttttgcg catttgtaaa
    10381 taaagcaggc aggtctgcga ttgtttctga attcagattg acagtgtccg gggaatatga
    10441 taaaatgaaa gagataggga aaaccggcgt gatgccgggg attattccaa gacagaagaa
    10501 tgaaaaaatg cagcattcgg ctgcagtcag tttctgctga gttagcggag gaaacaacaa
    10561 tgataaacgg atgcaaggtg gctgcacttt gtgtgtcaga aatacaaaat gaaaatacgc
    10621 agagcatgat ctatcccatg tatcaatctc ttctggaaaa gaactggcgc tgtctcattt
    10681 acaacacggc aacagatctt ttccgggata caccgtttga ccggggcgag gcgagcgtct
    10741 ttcagctcat ggatttttcc gtggtcgatg ttgtgatcgt atatacccag tgcctgaaat
    10801 gccgtgccat cgtggaggat attctggcgg cggcatcgca gcatcaggta ccggtgtttt
    10861 tggtagagcc gacagagcgg tattccagcg gcattcgcat cacctttgat gaactggatg
    10921 cgttccgtga attggtgacc catctgatcg aacatcatca tgtgacgaag tttgcctgca
    10981 ttgccggatt ccggggcaat ccggcatcgg agacacggga gatgatcttc cgggacgtgc
    11041 tgaaggaaca cggcattgca tttgatgaaa aatggtttgg ctacggcgat ttttatgcgt
    11101 tcccaacaaa ggcgctgatg gagcgctttc tggctgagcc ggagggactt ccggaggcga
    11161 tcgtctgcat caatgattcc atggcgatcg ctgtgtgcga ggtgctgtca gaccatggct
    11221 acagcgtgcc ggacgatgtg atcgtgacgg gatttgacgg cattatccag gaacagtaca
    11281 acttcccgcg gctgacgacc tgccgcagag atatgaaaaa actaggcgca tatatggcgg
    11341 aactgctgga gcgttcgctg tctgatacgc ccatgaaaca ggagtatatc tttccgtata
    11401 cactggatgt ttcgcagtcc tgcggctgcc ggaaatgcac catggagtcg gtcagccgtg
    11461 cggtcaatgc catctattcc cggatgaacg acagcgaaca gtacgaccgc tctatgaaaa
    11521 atatgctgac caagctgact tttgaacacg attcggcgaa aattcatgag atcctgcggt
    11581 actatatccg ttcggacagc tacctctgca tgaactcgga tttcgaggac gacaacccgc
    11641 cggagcatac ctatgaggaa cagccgttca cggacgttgt actgtcgcat cggtttttct
    11701 acggggaaca gaccacggag acggcgtata ttgcgaaaaa gtcgctgatc ccggattgga
    11761 gccgcctcat cgagaagccg gatccggtgg ttttctattc gatccacaat cagcagatga
    11821 tctacggcta tctggcagtg ttcgcaaatc cgttcaatta tgcggtacag cggatgcagc
    11881 agtttgtgct gacgttcgac agctgtgtgg ggatgtttgt acagcaggaa ttcctgcgca
    11941 gttccaacca gcggctgaaa gagatccaga acaaaatcat catcagcttt gcggatatgg
    12001 tggagtccag agacgatttc acgggacagc acgtcaagcg caccagcgaa tacctgcgca
    12061 ttctggtgaa gtatctggcg gagatgccgg agtatgccaa tattctgaca ccggagatgc
    12121 aggaacggat gtataaggcg gcgccgctcc acgacatcgg caagatcaag atctcagatg
    12181 tggtgctgaa taagccgggc aagctgacac cggaggagtt cgaagtcatt aagacacacg
    12241 cagaatccgg cggcgagatc atcgaaaaga cacttacaga cattgaaagt gactcctatc
    12301 ttccagtggc acgggaaatg gcgctgtatc accacgagaa gtggaacggc acaggctatc
    12361 cctaccatct ttccggagag gatatcccgc tctgcgccag aatcatggct gtagtggatg
    12421 tgttcgatgc actgaccagc aagcgtgtct ataaggatgc gttccccgta gagaaagcgt
    12481 tccggattct ggaagagtcc agcggaagtc actttgaccc gaagatcgtg gggacattcc
    12541 tggcacacaa ggaagaagtg acacaagttt tattaaaata tcaggatgta catatatgaa
    12601 tgcgttttcc agaacagaat tactttttgg cgcagatgcc atggaacggc tggcgaaagc
    12661 ccgtgtagca gtgtttggtg tcggcggcgt tggcggctat gctgttgagg cgctggcacg
    12721 gagcggtgtg ggcgcactgg atctggtgga tgatgatacg gtctgcgaat ccaatctgaa
    12781 tcgtcagatc atcgccaccc gtgccaccat cgggatgccc aaagtggatg ccgcagagga
    12841 acggatccgc ctgctgaatc cggactgccg ggtgacaaag catcagaagt tctatttgcc
    12901 ggagactgcc ggggagtttg acttttccca gtacgattat gtgatcgatg ccatcgacac
    12961 tgtttccgga aaactggggc tggcggaaca ggcatatgcc agcggcacgc cgatcatcag
    13021 tgccatgggt gcaggaaaca agctggaccc gacggcgttt gaagtggcag atattatgca
    13081 gacatcggtt tgtccgctgg cacgtgtcat gcggcgtgag ctgaaaaagc gtggtattcc
    13141 cagactgaaa gtggt
//
LOCUS       DAWENO010000047        13155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_1984_length_13155_cov_13.941221, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000047 DAWENO010000000
VERSION     DAWENO010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1984_length_13155_cov_13.941221"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    638..647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2760 a         3797 c         3135 g         3453 t
ORIGIN      
        1 actaccgctc ccgtgcgaca gctttattat tataccacgc tattttgact ttgtcaagta
       61 aaatcgatgt aaaatattcc tgttgaattt tgtttatttc gctaatagtc gtaatcgccc
      121 tcatcgtcgt cgtaatcatc aaaccagttg gtatcgtagt catcataatc tgcgccctcg
      181 tattcatcgt caatttcatc ggtatcgctg ttatactccg gaaaatgata agaatactca
      241 cgcttgacct tgctgctgga atcctcatgc gcactggtct catcctgact ctcttcatag
      301 aaggaatcaa aaacgctcgg ctctgacggt gtatccgcac acaactttgc gataatatag
      361 atcaaagaaa tcacggcaag tatgactgca attttttgtc cgtccatata tcctcccgta
      421 tcaatatatc ataattcaac taacttttca taaaccaaaa caacttggtc ttgtgttgaa
      481 tttacgccac tagaataatt tccaacacct attgtcatag aattcttccc taattcattt
      541 gtaatcattt cttttaagcg atagccctgc tctccatgcc aggttaaact tctctttaac
      601 gcctccacat ctgttccgcc atgccgatta tcataaannn nnnnnnngtg tcttgatatt
      661 tccgttctcc aaaattgttt ttcagttctg ccagttgttc caatatcttt aactgtaaaa
      721 atgttgccag atcttcttct ctgacatcct gtaaatcatt gtgttctaca gctttttctc
      781 gatttacagt tattttttcc tcatttgatt cgtttaccgg gaactttctt gctgctatct
      841 ccacatactc tggtgacatc cgataaccat tttctgttag atacttgtat accatttttt
      901 tgtagtatct tgttccatcg ctaagaaccc caatattttc tttatcattt gctcctattt
      961 cctcttgata tttctctaca gatattttca tatccaaagc gtccatttct tttaaggtct
     1021 tctctatcat ttccctcagt tcgctcataa gttgattccc cttttataca ttgtttcttt
     1081 ttttattata caattccaat ttcttgttgt caagtatttc ataataaaaa accgcctgcg
     1141 gaacccagtc gttccacagg cggtttgcct tatctgtatt tttattggct cttacgccat
     1201 cagtttcacc ataacagcct tctgtgcgtg cagacggttc tctgcctctt caaagatctc
     1261 gtttgcgtgt gtctcgaata cctttgtggt gatctcttcc tcacggtgtg ccggcaggca
     1321 gtgcagcacc atagcgtcct cgtgtgcctg tgccatgact tcgtcgttga tctgatagcc
     1381 gtggaatgcc ttcttccgct tttctgcctc gccttcctgg cccatggatg cccatacgtc
     1441 tgtgattacc acatcggcgt ccttggcagc ctccagcggc ttgttggtca tctggaactt
     1501 gtcgccgtac tgtgccgcaa agtccaggac ttccttgtcc ggctgatagt cgtccgggca
     1561 ggcgatagct acagacatac ccaccttcag acagcccaca atcagagagt ttgccatgtt
     1621 gttgccgtcg ccgataaagc acatcttcaa gccgtcaaag ctgctcttga attcccggat
     1681 ggtcatcaga tccgccagta tctggcacgg gtgacagaag tccgtcagac cgttgatgat
     1741 cgggatcgag ccgtactctg ccagatcttc cacttctttc tgctcaaagg tacggatcat
     1801 gatgccgtcc agataccggg acagtacacg tgcggtatcc tgtaccggct cgcctctgcc
     1861 gatctgcaga tcgtttgcag acaggaacag cggatgaccg cccagctggt acataccggt
     1921 ctcaaaggaa acacgggtac gggtagaagc cttctggaag atcatgccca gagacttgcc
     1981 cttcagatgg ggatggggaa tgccgtgctt cagctcatat ttcagctgat cggcaagatc
     2041 caggatctcg atgatctctt cggtgctgag atccagcatt ttcagtaagt gtttcatagt
     2101 ctaactcctt catttaggaa acactgcata aagcttgtgc gtaagatgcg tagtcagatt
     2161 tttcggcaga ttgcatagaa atttcgcagg catacttgat gtatggcaag ggatttctgg
     2221 gcaatatgac ggaaaatctg caagcagatt gcgtgcaaag ccgtcaggcg ggctttatgc
     2281 agtgtttcct ttatttcgac agaacgtccg ccaggatggc aacgcccttt tcgatttcct
     2341 cgtctgtgat ggtgagcggc ggcagcagac gcaccttggt ctttgccgtc agcaccagca
     2401 gtccctcttc cagcgccgca tttgccacgt cgatggcttt cttgtccttg agggcaatgc
     2461 cgatcatcag ccccagaccg ctgacgctct ccacgcccgg caactgtgcc agcttttccc
     2521 ggagcagtgc tgccttgcgc tttacctcgt ccaggaaccc cggcgctgtc accgtgttca
     2581 gcacagccag accgcctgca cagcagaccg ggttgccgcc gaaggtcgag ccgtgagagc
     2641 ctttgtccag cacgccggcg cacttctcac tggcgagaca agcgcccatc ggcagaccgc
     2701 ctgcaatgcc ctttgccagc gttgtcacgt cggcatggtg tccgaactgt tccccggcga
     2761 ggaatgtacc ggttctgccc acaccggtct gtacttcgtc tgcaatgacc agcacgtcct
     2821 ttgcggcgca cttttcaaaa acagcgtcca caaatgcctg ttccagcgca ttcacgccgc
     2881 cttcgccctg gatgtattcc agcatcacag cgcagactgt gtcgtccagc ttggactcca
     2941 ggtcggcaat gtcattggca actacatagt caaagccctc cacgaacggg aagaagtagt
     3001 tgtggaacac gtcctgtccc gttgcagaca gggtgcagag ggttctgccg tggaacgaat
     3061 tcaccagggt caggatcttg tgtctgccct tgccgtattt gtcaaagctg tactttctcg
     3121 ccagcttgat ggcgcattcg tttgcctcag cgccgctgtt gcagaagaac accttttcat
     3181 agcctgcggt ctcgcacagc ttctgtgcaa agtccgcctg caccttggtg tagtacaggt
     3241 tggaggtgtg ctgcatggtc ttgacctgcc ggcacaccgc ctctgtccac gtctcgttgc
     3301 agtagcccag cgaagtcgtg ccgatgccgc tgccgaagtc gatataggtc tttccgtttt
     3361 catccgcaca ggattcccct gcgccgtggt cgatgaccac cggaaaccgt ccgtatgttc
     3421 ccataacgga ctggttcatt tgctcttgtg ttgtcatttt catcatttcc tttattctgc
     3481 cgcctgttca gcggaaaatt tctgttccag aaagtcgatc agttccatgg aattgggaca
     3541 gccctgtgaa aagccgtact tcttgccgtc cagatacagg atgatgcact gatcttcgat
     3601 gtacatttgt tccagttccg tccaggcgta gtgcttttcc ggtttccagt tctgccggat
     3661 acggatgcct tcctcatccc acatgacccg gaaccgcagt gtgcagctga aaaagtacag
     3721 gctgagcagc aggaaagcga tcatcagcag cgtgtaccag atctcgcttg tgccgtaccg
     3781 cacaaagaaa gcgatcgttc cagccagaaa tactgcgacc ataaagccgt agatcccata
     3841 atcttttctt gcagatgtca gtttcatgcc tttttctcct tcatatagtc acgcagatat
     3901 gccagaaacg catccgaccc caggaaaaag tccttttcca cccggatttt tcccttggcg
     3961 gtcagaacgg tatagccgtc gcttttcagt tcgatcttct gcacgtcctg caattcatac
     4021 tgtttcggct ttccgaagaa gttgtatacg gtgatgccgg tgcggtcata ttcgatcttg
     4081 aaattgtgac cgtagatcag cagaaatacg cccagcagca caaacgctgc cgccaacaca
     4141 taaaacatca caccgccctg ctttaatttc agcagtgcca ttgcaatacc catcagaacg
     4201 gaaaacactg agaccaccgg aaacaccctg gacgctttca ccacgcctct ggtgtccgtc
     4261 cggttctctt cgttttccgc aaatttcttt gccgcctcta ctgcggcgtg ttctaacaaa
     4321 ctcatttttt caaaaaccct ttctgctccc cgtccgcatc acttccgaca cagaccagcc
     4381 gcctgtggaa aggaggcagg acgagctgtt ttttgccggt atcccccggc tgacgcttat
     4441 cggaactgtg ttccgatgcc ctcattggac agggtctcaa tgaggatgga gtgcggtact
     4501 ctgccgtcaa tgatggcagt ctttttcacg ccacggcgca ccgcctctac gcagcagtcg
     4561 atcttcggga tcatgccgcc ggagatgatg cccttgcgct tgaggaacgg cacttcgctg
     4621 acgttgacaa acggaatgag ggtagacgga tcgtccttgt cccggagcag tccggcgatg
     4681 tcggtcatca ggatcagatt ttccgcccgg agctgtgcag cgatccgtgc cgccgccgta
     4741 tccgcattga cgttgtaagc ttgtccgaaa tcgtcggtcg ccaccgtagc aattaccgga
     4801 atatagccgt tgttcagcgc atccaggatg ggctgctcgt tgacctttac gatatcgccc
     4861 acaaagccca gatcttcgcc gttgggaccc agcttgcgct ctgcggtcag catatgccca
     4921 tcgatgccgc acatacccag tgccttgccc cgtgtccgtg tcagcaggct gaccagctcc
     4981 ttgttcacct tgccggacag caccatcttt accacgtcca cagccgcctc atcggtatac
     5041 cgcagaccgc cgataaactt ggactcgatg ttcagccgct tgagcatatc gctgatctcc
     5101 ggaccgccgc cgtgtaccag gactaccttg acgcccacca gggacagcag cacgatatcg
     5161 tccatgactg cctgtttcag ctcgttgttg gtcatggcgt tgccgccgta tttgaccacc
     5221 agaatcttgt tgttgtattt ctgaatgtag ggcagggcat ccaccagcac ctgcgcccgc
     5281 acactgttca atatctctcc cattttcgaa agcctctctt acgaacggta gtcaccgttg
     5341 atttttacat agtcataggt cagatcgcag ccccacgccg ttgccgccgc cggaccgtcg
     5401 ccgatggaca ccagaatctc gatctcgtcc tccagcagca ccttcttggc gatgtcctcg
     5461 gagaactcga tgcctgcgcc gtttctgcac acatccacag aaccggcacg ggatgccagt
     5521 gtcactgcca cctggttgat gtcgaactct gccggagcat agcccacggc acacagcaca
     5581 cgtccccagt tggcgtctgc gccgaacata gcgcacttca gcagaggcga gcataccacg
     5641 ctctttgcca cggcaatggc agtgtccaga tcctttgccc cggagacctt gcactccagc
     5701 agcttggtcg caccctcgcc gtcatacgcc agcatccggg tcaaattcat catgacgcag
     5761 tacagggcgt tccggaacac ggtatattcc gcagaatccg ccgcaacttc cggattgcct
     5821 gccgcaccgg atgccatgag cagacaggtg tcgttggtgg actggtcgcc gtcgacagac
     5881 agacagttat aggtcacctt caccacgtca gacagtgcct tttgcagcag ttctgccggc
     5941 acagccgcat cggtcgtgat gcagttcagc gtggtcgcca tgttcgggtg gatcatgccg
     6001 ctgcccttcg ccatgccgcc caggtgacag gtcacgccgc ccagtgaaaa ttccacagcg
     6061 atctccttgg cgaaggtatc tgttgtcata attgccacag ccgcctcgtg cgcaccctct
     6121 gcgctggctt tcagatttct gcacagcgcc ggcagggcgt tctgaatcgg ctcaatgggc
     6181 aggatctgcc cgataacacc ggtagacatg accagaatct cttccggctt gacacccagt
     6241 tctttggcag tcagagcgca catagtctcc gccttttcct cgccgtctgc gttgcaggtg
     6301 ttggcgttct tggagtttgc caccactgcc atggtcttgc cgcaggtggc tttcagatgc
     6361 tttttcatca ccagcaccgg cgcacccttc accttgttct gggtgtagac cgcagccgca
     6421 ttgcacatgg catctgccgc aaccaccatc aggtcatttt tcttgtccgg gttgtccttg
     6481 atgccgcagt atacgccgcc ggcacggaat ccctttgcgg cacaaacgcc gccgtccacg
     6541 aaggtatagc cttccattgc tactttttcc atgaggtatg actcctctct gattgatttc
     6601 gatttctctt tttcaagaca gcatcgtaca aagccgacag gcgggacgat gtgggcatcg
     6661 tcccctacta tgccgtttct ctaaaagcta tctgtaatgc cgcccatagg cacataaatc
     6721 ggtttttaag aaagtccccc ttgaaaaggg tgattcccca cagtgtgggg aaatgtcacg
     6781 aagtgacaaa ggggacggct cggtaagaga tgtcagcgta agctgacagg gggattattc
     6841 tgtgaaatcc acaacttgaa gaaaaatttc acccaaacct ctccaccagc ttcgctggtc
     6901 cccctctcct ttcaggagag gctatttttt tacagcaagc ccacagtctc atccagtccc
     6961 agcataatgt tcatgcactg cactgccgca ccggatgcgc ccttacccag attgtccaga
     7021 cgggaacaca gcagaatctg ctctgcgtca ccgaacacga aaatctccat ctggttggtg
     7081 tcacgcaggt cgttgcagct cagcttggtg gctgtcatct ctgccggcgt tttcagcggc
     7141 tgtacatgga cgaagttgga gccctcatag tggcgttcca gcatctcctg tacccgttcc
     7201 ggttccagcg aaaagccgga gaccgccgcc tggatcggca cgcataccac catgccgtta
     7261 tagtaatcgt ccacgatcgg ggtgaacatg ggtttctttt ccagaccgga aattttctgc
     7321 atctccggca ggtgcttgtg gttgtttgcc agaccgtaaa agcgagggct gtacagctcc
     7381 ggcgcacggt cgggcgattc gatctcggcg atcatcttct tgccgccgcc gctgtagccg
     7441 gacatggcaa agcactgcac cggataatcc ttaggcagca gtccgctgtg taccatggga
     7501 tacaccagag acgcaaagcc gctggcataa cagcccggca ctgccacacg cttggaggtg
     7561 cggatctttt cccggtgttc cggagacagt tccggaaagc cgtatgccca gtccggattg
     7621 gtgcggtgcg ccgtagatgc gtcaatgatg cgcacatccg catcccctgc cagcgccaca
     7681 gcctctcttg cggcatcatc cggcaggcac aggaatgtca cgtcgctttc cagaatggcg
     7741 gcacgtcttg cctccgggtc tttccgggtt tcctcggtca gggtaatcat ggtcagatcc
     7801 ttccggtctg ccagacgctc atggatacgc agaccagtcg tgccggcctg tccgtcaata
     7861 aatactttta cagccatttt tccacctcat tcccctgccg cacaattcgg gcagggcgtt
     7921 tttccgccga cgatcattgt tttcggcgat tttgttatgc gtggcttcgg tgcaggcatt
     7981 gtctctccct gcatattgta cacaacgtgg acataatcct cttcggcgca ttcctccgat
     8041 agatgccaat aagagccagt tgcactatag tataccattt ctatggcagt cgtactctgc
     8101 gtttccacag ttgtgatctc ctgcttttcc acagcatcca gcgtttgctt tggagtcacc
     8161 gccgtcagct ttttcccgtt cggcgcatcc acagccttct tcttggtcgt cactgccgcg
     8221 gcatcgtcct cctgcacagc agcagttgtc tgcaccgcat ccagttgtac ctttgccgtt
     8281 gtcactgcgg acgatgtcac cgtttccgaa gcttcaggcg aagccgtcct gccgcagcca
     8341 gcgcaaaggc agaacccgac cgccatcagg atcccgatcc attttctcat aatttctcct
     8401 attcccgtgg acacttccac agactaaagg cacaggtcag cacgatcacg gcaatcgccc
     8461 agatccggga caccgtcagc cccagatcca gcaccagacc cagcaggatc tctccgccga
     8521 tccagaccgc caccgccgcc gaacacagcg ccaggataaa cttcatctgc cgctttttca
     8581 tgccagcccc agttttgcct taaccagctt cagttgttct gccacagcct tcggggacgg
     8641 accgccctga gaaactctgc cacgcacgca ggtctccagg ctgatggcat cataaacgtc
     8701 ctcggtgaac agcggtgtca tcttctggta ttcctccagc ggcagtgtct ccagagtcag
     8761 acccttggaa atgcactctg ccaccagtgt accggagatc ttataggcat cccggaacgg
     8821 catacccttc ttgaccagat agtctgcgca gtcggtggcg ttgataaagc cctttgccgc
     8881 agcggcacgc atattgtctt tcagcacacg catagtctcc agcatggggg tgaaggtctc
     8941 caggcacagc tttacgttgt ccactgcgtc gaagatcgcc tccttgtcct cctgcatatc
     9001 cttgttgtat gccagcggca gacccttcat catggtcagc agggtctgga gatcgccgta
     9061 cactctggcg gtcttgccac ggatcagctc ggtaatatcc gggttcttct tctggggcat
     9121 gatggacgaa ccggttgcaa aggcatcgtc cagttcaatg aacttgaact cccaggaaca
     9181 ccacaggatg atctcctcag agaaccggga caggtgcatc atgatgatgg aaagggtgtt
     9241 tgccagctcg atgcagtagt cacggtcaga aacgccgtcc aggctgtttg ccataggcgc
     9301 atcgaatccc agcagggatg cggtctcttc ccggtcgatg tgataggtcg tacctgccag
     9361 agcgccgctg cccagagggc attcgttcat gcgccttgcg gtgtcgtcca gtctgcccag
     9421 gtcacggagc agcatctgca catacgccag caggtggtgt gcaaaagtca ccggctgcgc
     9481 acgctgcaaa tgggtgtagc ccggcagtac ggtctcggtg tgctgttctg ccagcttgac
     9541 cagtacggcg cacagctttt ccaccagacc acgaatttcc ttgatctcgt cccggagata
     9601 cagccggaga tccagtgcca cctggtcgtt tctggaacgg gaggtgtgca gacgcttgcc
     9661 cacatcgccg tagcgcttgg tcagttctgc ctccacgaac atatgaatat cttctgcgtt
     9721 ggggtcaaat tccagcttgc cgctgtccag atctgccaga atggcagtca gcccctcgat
     9781 aatggtcttg ctgtcctcct tggagatgat gccgcagtca cccagcatgg tggcatgggc
     9841 gatactgccc tggatgtcat gccggtacat ccgtgcatca aatgcgatag aggagttgaa
     9901 cgcatttacg cctgcgtcga tctctttgga aaatcttcct gcccaaagtg ccataatttt
     9961 atccgtcctt ttctgattct gtatattccg tgtgcggatg atagtgtgcg atccactgct
    10021 gtgccagcct gtatttttct tccagcggtt ccatatcgtc aaacaaaagc gcacgccgct
    10081 cagtttctgc atgatctttt ttcacgcaga tctgcaaaga tatcatattc cggtttccgt
    10141 aatgtgtgca gtacagctgg attcgctgta tctcttgcca ggaaaacgac tcgaatcgct
    10201 gtatctcctg ccaggaaaac gactcgaatc gctctcccca ataacaaatg ccggagggcg
    10261 acagcctcag actgcatggt atagctttct ttttctcttt atagagggcg tgtaaggcac
    10321 cgcacataac gccaatgccg acagcagccc agccaaaaat ggtaagcaca caaagcagca
    10381 gcacagaaac tgccaccatg atctcctcac gccggcggcg aaaagccatc tgttcccatt
    10441 ctactttttt gtaaaagacc agcgtcggct ctgccgctgc tttgcttttc tttttcatac
    10501 acccctcaca acaacaaaag gcgactgaac gccgcctttt gcctccggca aaatcaccgg
    10561 tttttatttt cgctgattgg cttacttgtt gttccgcttc tgatccagta cagcctttac
    10621 gtggatcggc agaccgaaca ggttgataaa gccctctgcg tccttctggt tgtaaacgtc
    10681 ttctgcatcg aaggttgcaa cttcttcatc atacagagtg tacggagaag ttacgccggc
    10741 attgatgatg ttgcccttgt acagtttcag cttaacatca ccggtaacgg tcttctgggt
    10801 ctcgtttacg aaagcagtca gtgcctcacg cagcggagta taccactgac cgttgtatac
    10861 gatatcagcg aacttgatgc tcagatactg cttggttctt gcagtttcct tgtccagggt
    10921 aatggtctcc agcacatcgt gtgcgtggta caggatggtg ccgcccggag tttcataaac
    10981 gcctctggac ttcatgccga ccagacggtt ttctaccagg tcagccagac cgatgccgtt
    11041 tgcgccgccc agttcgttca gcttttctac cagctctact gcgcccagtt ccttgccgtc
    11101 gatagcggtc gggataccct tttcaaaatg aatggtcaca taagtgggct tgtccggagc
    11161 ctggatcgga gaaacgccca gttccaggaa gccttccttc tcatactgcg gctcgtttgc
    11221 cggatcttcc agatccagac cttcgtggga cagatgccac aggttcttat ccttggagta
    11281 gttggtttca cggttgatct tcagcggaat gttgtgtgcc tctgcatagt cgatctcttc
    11341 gtcacggctc ttgatggtcc attctctcca cggagcgatg attgccatat ccggagcgta
    11401 tgccttgatt gccagctcga aacgaacctg gtcgttgccc ttgccggttg caccgtggca
    11461 gatggcgtct gcgccctctt ccagagcgat tcttgccagc tccttaccga taatcggacg
    11521 agcgaaagag gtacccagca ggtaacggtt ctcatatact gcgccagcct gtacagtcgg
    11581 gaagatgtag tcctctacga aggtcttctt cagatccagt acgatcagct tggatgcgcc
    11641 ggtcttgatt gccttttctt ccaggccgtc cagttccgta ccctgtccta cgtctgcgga
    11701 aactgcgata acctcgcagt tgttgtagtt ttctttcagc cacggaatga taatcgaagt
    11761 atccagaccg ccggaatatg ccagaacgac tttcttaatg tccttttttg cctttgccat
    11821 gatgcaaact cctccatcag cataatttat cgcatctgta ccgccctttt tgatccgctg
    11881 caaatgctct tgtcttttat tatacacctt ttttcagcga tgtcaagcat ttttgcataa
    11941 atatacggat tttttttcct tttgcaatat tttacgcata atatgcagtt attcctgtat
    12001 atttttatgt ttccgtgaaa aaaatgcagt gcaaagcgcc tgctccacgc aaaaaagccc
    12061 ctgtcacagg acagaggctt tttgatttcg ggaatactca gtgcagtttt tcataatatg
    12121 ccagtacttc acccacaaat tcccggtgca tcgggtcggt gccgtaccat ctttcatagg
    12181 tctccggttc gatgaagccc tcgggctgca tcatcgccgt atagcgtttc cggcagatca
    12241 gcacacggcg tgcctgtgca aaggcagtgg ttccgtccag gagaacgggc cgcaatccgg
    12301 tttgtttcgc cttatcgcag tcccggccgg aatgtgtgcc gcagaattgc agtgccggtt
    12361 tatagcccgg atcgaaaaag cacgctgaaa acagttcgtt ccgatccaga tattgcaggg
    12421 tataccgatt ggtgcgtacc acacagtgga aggacggtgc gccccagatc tcacccatcg
    12481 caccccagga tgccgtcatg gtgttgtagt cctccggtgt gccggatgtc agtaagtacc
    12541 atgattttcc gatcatgtca aaaggattgc actgtagatc gcttagctcc attttcttaa
    12601 aaaacatttc tccgtctgtc ataaaatcaa ttcctttcgc ataagataat actatgtctc
    12661 ccggcggaca ggcagtgcag agagcagata gctgttttta ttgtaccaca aaacagagcg
    12721 ctactgtaag aagattttac gcagagaaaa taaatttttt cacaaagcgc agcgattttc
    12781 aggcgtgatg ccgcataccg ctctttccgt actgcagccg cagcttgtcc gtgagcagcg
    12841 caatgaattc cgagttggtg ggctttccct tgcagttatt caccgtgtag ccgaaaaagg
    12901 aattgatggt gtccaggtcg cctctgtccc atgcgatctc aatggcgtgg cggatggcac
    12961 gctccacacg ggaagaggtg gtgtcgaact gctgcgccac cgtgggatag agcatcttgg
    13021 tgacgctctc cagcagtgcc tggttctcga tggatgccag aatggctgca cgcagatagt
    13081 gatagccctt gatatgtgcc ggcacgccca gctggtggat catctccgtc accaccatct
    13141 ccagtgtccg gtgcg
//
LOCUS       DAWENO010000048        12896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2037_length_12896_cov_10.043299, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000048 DAWENO010000000
VERSION     DAWENO010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_12896_cov_10.043299"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2960 a         3429 c         3827 g         2680 t
ORIGIN      
        1 atcggaaagg gtctgttcat cgaccacggc atgggcgtgg tgatcggcga gaccaccatt
       61 atcggtgaca actgcctgct gtaccagggc gtgactctgg gcggcaccgg caaggacaaa
      121 ggcaagcggc atcctaccct gggtgacaac gtgatggtgg gcgccggcgc aaaggtgctg
      181 ggtcctatca acatcgggaa caacgtcaag gtcgctgcca atgcggtggt gctgaaagat
      241 attcctgaca actgtaccgc cgtgggcgtt ccggcgagaa tcgcccggat ggcaggtcaa
      301 aaggtacagc agcaggatct ggatcagatt cacattccgg atctgaccat ggaggacatc
      361 accatgctga aatccgcagt gacttgtctg cgggcggaga tgtctaagct ggaaagccag
      421 gagaaaaagg aaaagcagga aagcgaaaat tgatcggagg atacagccat gcagtgtgtg
      481 gtattcgata tggacggcgt gctgttcgat tctgaggcgc tggtactgcg cagctgggaa
      541 cagaccgcag agatccacgg cattccagat gtccgggagg tctgtctgcg ctgtctcggt
      601 acaaatgcgg cagaatcccg gcggctgttc ctggaaaaat acggggcaga ttttccctat
      661 gacacctata aggcggagat gtctgcggga aaatgtcggc gaaggcaagc tggaactgaa
      721 acccggcgtc cgggagatcc tggacgccct gcggcagaac ggctggaaaa ccgccatcgc
      781 ctcttccacc aggacggcag ttgtccggga agagatgcag catttcggtc tggaacagat
      841 gttcgatgag atcatcggcg gcgatatggt gcgccgcagc aagcccgacc cggaaattta
      901 tctgacggcg tgccgggtgc tgggtgctga cccggcagag acctatgcgg tagaggattc
      961 ctacaacggc atccgttccg ccgctgccgc cgggatgcgt ccggtgatga tcccggatct
     1021 tctgccgccg accccggaga tcgaatcgct cacggcaaag atttttccgt ctatgacgga
     1081 atttcagcaa tttttatttt cgtaaaaaac ggcagaggca ttctgccgaa aatccataaa
     1141 aggagtcaat cgtcatgcag atctataata cgctgacacg aaaaaaagaa gagatcgttc
     1201 ccattcagcc cggacacatc ggcatttatg cctgcggtcc gacggtatac aactatatcc
     1261 atatcggaaa cgcccgtccc atctgtgcat ttgatgtgct gcgccgctat ctgaaatacc
     1321 ggggctatac tgtcacctat gtgcagaatt tcactgacgt ggacgacaag atcatcaaaa
     1381 aggcaaacga agagggaacc actgccgcag agatcgccaa gcgctatatt gcggaataca
     1441 agaccgatgc acacggactg aacgtcatgg atgcggatgt acaccccacc gtcaccaact
     1501 gcatgaatct tatcattgac attgtgaaaa agctggtgga ctccggtcac gcttatgccg
     1561 ctgacaacgg agacgtatac ttccggacct cttctttccc ggaatacggt aagctgtccg
     1621 gccagcccat tgaagaactg caggcaggcg caagaatcga catcaacgac ggcaaggagg
     1681 aacccctgga cttcgctgtc tggaaggcag cgaaaccggg tgaaccctac tgggattcgc
     1741 cgtggggcaa gggcagaccg ggctggcaca ttgaatgctc tgccatgtcc tgccactacc
     1801 tgggcgagac cttcgacctc cactgcggcg gtcaggatct gatcttcccg caccacgaga
     1861 acgagatcgc acagagtgag gctgccaacg gcaagccctt tgcacactac tggatgcaca
     1921 acggctatat caacatcgac aacaagaaga tgtccaaatc cctgggcaac ttctttaccg
     1981 cacgggaagt ggcggagcag tacggctatg aagtcatccg ctacatgatg gtgcaggcac
     2041 actaccgcag ccccatcaac tattgcaagg aactgctgga tgcctgcaag gcgtcgctgg
     2101 aacggctcta tgcctgccgt gataccctgg atcgggcaat cgctgcggcg aagtccggcg
     2161 aagttgcacc ggaggcggca gagacgtttg ccaagcgcaa ggaacagttt atcactgcca
     2221 tggatgacga cctcaacacc gcagacggca tgacggctat ctttgaactg gtgcgtgatc
     2281 tgaaccgcat gagcgcagac gctgccactt ccaaggaaca gctggaggca ggcgcaaagc
     2341 tgttcgatga actgatcggc gttatgggtc tgctgtacaa ccggaaaaag acagacgaga
     2401 tcccggctga ggtgaccgaa ctggtggaac agcgcaaggc agccagaaag gcaaaggact
     2461 ttgcggaggc agaccgtctc cgggatgcca tcgctgcact gggctatgcc gttcgggaaa
     2521 cacggcaggg tactgagatc acaaagatat aacaaagtcc gcccggcggc tttgcatatg
     2581 attttcccga aaggatagcg aatggcactc aaacaacata aaaaagcaat cacaagaatc
     2641 tttatcttaa tggtcgtaat gacactgctc tgcgtgggcg tgttctcctg caccacctat
     2701 atggagcagg agcgcaagac ctttgtggac atgagccagg tggaactggt gcagttggaa
     2761 acaccggcgg cggatgcacc ggcaatgaag atcaccacct ctgccggcac gatcgtggca
     2821 gagctgttcc cagagcaggc gccggcttat gtgaagcagt tcaccgaact ggcagagtcc
     2881 ggctattacg acaacaccta tgtgttctcc gtcgaggacg gcgtttactt tgaggcaggc
     2941 agtcccaacc cggacggctc gctgaacagt gcggacggcg acacctatga gcgagtggaa
     3001 cgggagacca gcggcgacct gtggccgttc cgtggggctt tctgtgtgcc gaccacggcg
     3061 caggagggca atctgtggga ccgcatcacc ggaaaaatga ccacctactg cgggacacgc
     3121 tttgtggtct gcaattccat cgtgtttgac gagtctgcga agaacgagct ggaatcggtt
     3181 tcggaaaacg ccgagaagat caaccaggcg tttctggaaa acggcggcgt gccgaactat
     3241 tcccagcaga tgaccgtatt tgcccaggcg tatggggacg aaagctttga caccatcgac
     3301 aagatcacca gcgctgcggt aaaggcatcc tccggagacg gcggctatac accgccggaa
     3361 gagaatattc ttatccagaa agtggaaatc ggaacatacg gcgcatttgc ttctgacgca
     3421 aaaacagcgg aataagggcg aatatccaaa ccgccttgaa aaatacataa tttcccttat
     3481 ggaatccata caaatgcgat ttcacatggc agaaattttc cgtcgatact tccaaatagc
     3541 gcaaaaaatg cttgcttttt actttacaaa tgcgcagaat tcgtgtataa taaggaagat
     3601 agcatacaaa ctataggcag caccatgcaa aacagccgga cggcttttgc accgtgtcgc
     3661 ctatattgtg tgcagaccag aaaaaatgat tctggataac cggaagaatg aagcggcttt
     3721 tttgccgccg gagaacagca gcctgccggc acaaggcacg ggcgctgaat agaggaatgg
     3781 gaatggacgg caggcgtgcc ggttttggca ttccccatgt gtcgccgtat ctgggcggtt
     3841 tcggttcctt tccgatgccg ttgcagcaat ggacaattgg gcgcacagaa aaaaggagag
     3901 acgaaattgg ataaatttgt cattcacggc gggcatcggc tcaccggcga aatagaagtc
     3961 agcggagcaa agaatgcagc ggtcgcactg atccctgcgg tgctgctctg tgatgaaccc
     4021 tgtatcctgg aaaatgttcc ggatatcagt gacgtttcca tcagcctgcg cattatgagc
     4081 gaaatgggtg cgcaggtgga gtatctgtca aagaccacag tccgcatttc tgcaatggga
     4141 ctgaccaact actgcgtacc ctatgagagc gcacggcgga tgcgtgcatc ctattacttc
     4201 ctgggtgcgc tgctgggacg ctacggcaag gcgagggttt ccatgcccgg cggatgtccg
     4261 ttaggcgacc gccccatcga ccagcatctg aaggcgtttt ccgcactggg tgcaaactgc
     4321 aacattgaac acggcatggt ggatctggtg gcagaccagc tggtgggcaa tcagatctac
     4381 ttagacgttg tttccgtggg tgcgaccatg aacgccatgc tggcggcagt tcgtgccaag
     4441 ggtctgaccg tgatcgaaaa tgcggcgaaa gaaccgcata tcgtagacct ggcgaacttt
     4501 ttgaactcca tgggcgccga tatcatgggt gccggcacgg atgtcatcaa gatccgtggg
     4561 gtagagcgtc tccacggcgc agtgtactcc gtcattccgg atcagatcga ggcaggcacg
     4621 tttatgatcg cagccgctgc cacaagaggc gatgtgctgg tgacaaacgt gattcccaag
     4681 catctggaac ccatcaccag caaaatgcgg aagatcggcg tcaacatcga agagtttgac
     4741 gacagcgtcc gtgtctgggt ggatggtccg ctggtgcgca ccaacgtaaa gaccatgccc
     4801 catccgggtt tccccaccga tatgcagccc cagatcacaa cactgctgac tctggcggaa
     4861 ggcaccagca tcattaccga gggcgtctgg gaacagcgtt tccggtatgt ggatgaactg
     4921 cgccgcatgg gtgcggatat ctctgtggac ggcaaggtcg ctgtggtgga aggtaccgga
     4981 aagctgaccg gtgcgccggt aaaggcgtgc gatctccgtg ccggagctgc gctgattatc
     5041 gcaggtctgg cggcacacgg tgtcaccgag atcgaggaca tttaccacat tgagcgtgga
     5101 tatgccaacc tggatgccaa gctgcggcag atcggcgcag atatccagaa ggtctctgaa
     5161 cccacagagg ctgctgcgtc tccggagctg aaagaagcgc tctgaatgac agccaaatga
     5221 aatgaaacaa gccgtcagcc tggttgacgg cttttgttgt ggaaaaacgg aggtctcaac
     5281 gtgtcgaaat tttactccct gttcagttcc agcaagggca atgcaagttt tgtgggcagc
     5341 cctgccggcg gtgtgctggt ggatgccggc gtttcctgcc gcagactgct gcagtcgctg
     5401 gcggcgcacg acatccccga aaaggcggtg caggcagtgt gtatcaccca tactcatacc
     5461 gaccatattg ccggactccg ggtgtttctg ggaaagcatc cgatcccggt gtacggtacg
     5521 gcagagacgc tggcacagct gcgcttttcg gataagttcc cccaggatgc tgatctccgg
     5581 gagatccttc ccacggagac catacatacc gacagcgtgg cagtgacggc gtttcccacc
     5641 atgcacgatg cgccgggcag ctgcggctat cgctttgaac tgccggatgg tcagacctgt
     5701 gcagtctgca cggatctggg ctgtgtcacg ccggaggtgg aaaagggcgt gctgggtgca
     5761 gacctggtgc tgctggaggc aaactacgac ccgaaaatgc tccgctatgg accgtatccg
     5821 gcgtcgctcc aggaacgcat ccgtggaaaa tacgggcatc tgtccaacgg cgacagcgcc
     5881 gggacggcgg aaatgctcat cgaacacggc acaacacgtc tcatactggg gcatctcagc
     5941 gaaaaaaaca ataccatgcc tctggcacag cagaccgtgc tggaacattt gcaggaacag
     6001 ggaatgcgcc ggaaccggga tttcctgctg caggtggcgt cgccggaggg cttagaacag
     6061 gcggtgatct tctgatgcag accatacaga tcctggcact gggaaagtgc aaggaaagct
     6121 atcttcggga tgcgtgccgg gaatacgaaa agcggctgtc ccggttttgt cagttacagg
     6181 tgacggagct ggaacccgtg tccctgcccc agaatccctc ggaaaaggaa attgcggcgg
     6241 cgctggaaaa agagagcgct gaactgctga aacgtgccaa aggcttttgc attgccatgt
     6301 gcatcgaggg aaaacagttg gacagtcccg gactggcaaa aaagctggaa caggcggcgc
     6361 agggcggcga cagcggcgca gtgacatttc tgatcggcag ttcctacggg ctgtcggatg
     6421 cggtgaaaca gcgtgcaaac ctgcggcttt ccatgtcggc aatgacgttt ccgcaccagc
     6481 tggcacgggt gatgctgctg gaacagattt accggagcta tcagatttta tccggcacca
     6541 agtatcataa ataggagtga tttttcatgc agaaagcaat gacgatcgcc tatgcagtag
     6601 gcagaaatct ctatttgaat attaccaacc gatgtccctg tgcctgcaca ttctgcatcc
     6661 gcaccatgtg cgacggcgcc tacggctcag accctctgtg gctggaacac gaaccgtcta
     6721 tggaggagat tcgggcggcg ctggacaagg aggacttgtc gcagtatgaa gaagtggtgt
     6781 tctgcggcta cggcgagccg acagagcgcc tggacattct gtgcgaaacg gcaaagctgc
     6841 tgaaaagccg tggggtaaag accatccgca tcaataccaa cggattgtcc gatctgattc
     6901 acggcagaaa aacggcggca gatctgaaag gtctggtgga tatcgtgtcc gtcagtctga
     6961 atgccgggac agaggcggaa tatctgaaag tcactcgccc caagtacggt gcggcggcgt
     7021 atccggcaat gcagcagttt gcgctggact gcaagcagta tgtgccgcag gtgatgttca
     7081 gcgtggtaga cattctgccg cagccggaac tggatgcggc gcagcagctg gcagaccgcc
     7141 tggggattca cctgcgcatc cgccagtttg acgatgcggc gaccagacgg gaaaagtgag
     7201 tcttttcgga gcagtactat gatggaaaat ttttccgaca tggtgctgct tttttgcgtc
     7261 cggattccgg ggatgtataa ttcttccgat tttgcagaca atagcagtac cctcgcagta
     7321 cactgcggaa ttgtaaggag gacttttgaa atatgaaagc aactggaatt gtccggcgta
     7381 ttgacgacct gggacgggtg gtcattccga aagagattcg ccgcaccatg cggatccggg
     7441 aaggcgaccc tttagagatc tacaccaaca gcgacgggga agtgatcttc aagaaatatt
     7501 ctcccatcgg tgagatcgga gagagtgccg cacaggtggc agatatcatg caccgcctgg
     7561 caggctgtcc ggtggcagtg ttcgaccggg atcatgtgat ttccgtttcc ggcgcagcca
     7621 aaaaggagtg gaatgccaga cgggtctcac cggagctgga agatctgatg gaacagcgcc
     7681 gccagtactt ttcagacacc ggcgagccgg attttcttcc ggcagagggt gtggaaaagg
     7741 ctgccgtggc gtgtatgccc attctctccg ccggagacgt gacaggagcg gtggcgtttc
     7801 tggaggacgg cgagcatacg tcactcaatg agacacagaa aagtctgatc caggcggcat
     7861 cgcagtttct gggaaagcag ctggaggatt aacgcagtac catatgcaaa aaacgggctg
     7921 tcggagtgtt ccgataaccc gtttttgtga tgcaatagaa ttttttatga cgtgttatcc
     7981 caactgtacc cggtacagac tcgccatgcc ggagaccacc agccgcaggt ctgcctttcc
     8041 ggagtaagcc tttagaggaa tggtgtacag ctggaaatct tccatgttcc gggacggcag
     8101 gatgtcgatg ctgcccaggg cagtttctcc ggcgtagacc gtcaccgtcg ccttggcaca
     8161 gggtgcagct gcccagagct ctgccttggt gtatccggcg aactcggaat ccgcaaaggt
     8221 gaaacttccg ccccagtcgt tggtgcagcc gtaccagtca tttttcccgt aggaaaagcg
     8281 gagtgtggtc tccatgccgt ccttgctgtc gtaattcttg acgcaggtgg gacgagacag
     8341 atcccggagc ggaatcactt cgccggaaac cgtgcattcc agttccaggc ggatgtctgc
     8401 cgaggatgcg cccacggaaa agcggtaatc gccctgttcc accagcattt tctcctgcgt
     8461 cacgtcgtag tattccaggg cgtaccaggg gacgaagatg gtcacgggga cggatgcacc
     8521 tgccggaacg ggaacacgct ggaatccgca gagctgccgg ttggggcgtt tcactctggg
     8581 gtgcagcgcc cggaaataca gctgaaccac ctcgtcaccg tcccggtcgg aggtgttctc
     8641 cagcgtcagc cggaacagca tcccctctgg cttttcctcg gcggtgaggt cgtggtagac
     8701 aaagctggaa taggacagcc cgtgtccgaa gggaaacagc ggcgtgaccg tgtcatacaa
     8761 ataggtgcgc tctgcctcga tgatgtcgta ttccatgatg tccggcagat cctgcgccga
     8821 gcggtaccag gtgatgggac accgggcagc cgggttgttt tcgcccagga gtgtggcggc
     8881 aacagcgctg cccagttccg gtccggcgtg ggtggtgtaa aggatcgccg ggacaagctc
     8941 ctgcgcctcg cccagagcgt agggatagct ggaaaccagg gtcagcaccg tgttcgggtt
     9001 ggcgtctgcc acagcccgga gtagcgcttt ctgatgcgcc ggaagttcca ggtcgggacg
     9061 gtcgtagcac tcccgtgcca cctgcatggg gtgattgccc aggcagagga tcaccgcatc
     9121 tgcctgcttt gccagttctg ccgcacgccg gatgccgttt tccaccggca ccagccggaa
     9181 cagccggtct gcggatggct tgccgtggcg gactgcccgg agcgtgccgt ctgtctggag
     9241 cgcaatgtcc atgggcttgt cgtgccagct gcggaaacgc atggcatcgc catagggttc
     9301 cggacgcagc cactcccgga tgaaccactc atacggggtg tcgctgattg ccttgtatgc
     9361 gccgtcctcg gtgacatact ttccgttgca gcaggcacgc aggttcatgc tgccgaagtc
     9421 ccagtcatgg agcaggaact tttcccgttc gccgacggtg tctgctgcgg cgtggagcgt
     9481 gccgtcctct gccacggaga aatacttgcc ggtctgcacc gactggagcg ccgttacgtc
     9541 gtagccgtca tcgaacagca cattttcctc gcccagctgt cggcggagtc cgtctgcaac
     9601 ggagatcgca taggaagaga tgccggtgta ccagtcccgg tagttctcgc tgcccagcgg
     9661 tccgatgact gccacttttg cgccgggtct cagatgcagc ggcaggatgc cgttgttgtg
     9721 gagcaggcac atctgttcca tggcggcaac tcggttgacc gcacggtgga gcagggtgtc
     9781 ggtctccgcc ggctcggtgt tgtaggggca gctgtcgccg tcgaattcgc ccaggcggaa
     9841 acggccggac agagagtttc ccacagcacg gtcgatgtct gcctcggtga ggagtcctct
     9901 gtccagggca tctcttgccg cagcggctac catatcatcg gcgtcggtca taacgtcgtt
     9961 accgtttttc agacaggcgg caagtgcctc ggcgtgggtg gcgtgggaac ggtgcgccag
    10021 tacattctgg gagaagtctg caccgtcggt cacgatgaat tccagacccc atttgtcttt
    10081 caccaaaggc tgcaagtcgg gattcatgca cgccggaacg ccgctcagtt cgttataggc
    10141 ggtcatcata gaaccggtgc cgccacggac gatggatgat tcaaatgcac ggtaatagta
    10201 ttcgtgcagg gtgcgtgggg tgacgttcga ggagcagagt atccggtctt tttcgttgtt
    10261 gttggcgcag aagtgtttca gcgtggggat ggtgcggcgg tataccgggt cgttgccagc
    10321 catgccctgg gtataggcgg tggtcatctc gccgatgagg aacgggtcct cgccgtagcc
    10381 ctcttcggtg cgtccccagc gtgggtcacg ctccgggtca actgtgggac cccagagcat
    10441 caaatgtccc ttggggtctt tccggtgata aaagcgagcc tcttcgccgg cgatctcgcc
    10501 cagctgttcc atcagattcg ggtcaaaggt ggatgccaga ccgataggct gggggaatac
    10561 ggtggaaggc tcttcggggt cacgggagac atagccacgg gcgacctcct gtcccacata
    10621 ccattctccg atgcccaggc gttctaccgg gagctggtgg gtggagagca gaccgatttt
    10681 ttcttcgacg gtcagacggg agagcaagtc ccggacacgt tctttcaagg gcagtttggg
    10741 gttgcgaaaa tcctgcataa tgttcctcct aaaaaagcaa gcctcctttg catccacaaa
    10801 gaaagcctgc tgtcctgtgt tggtatgatt atagcactgc cgccggggtg ttgtcaatag
    10861 aaattgtata aagtatcgca caaagaaaag gtgcagtgtc acaattagtg acagaggagt
    10921 ctgtgaccac gcattttttt gcacaaaaaa cagcagaccg aagtctgctg tacttatatc
    10981 tgaattccgt tttttgcgat tttctccgcc tgctttttca gccgcagttc ccggaaaaaa
    11041 tctttcagca cctgtgcgca ttccgcctgg agcagtccgg cgtgtacttc tggcgtccag
    11101 ttatagggca gtgtgaacag atgttccacc gaatccacag cgccgccttt cgggtcagat
    11161 gcgccgtaga cgacctgatc cagccgtgcg tgcaggatcg ctccggcaca catggggcat
    11221 ggctccaggg tcacatacaa tacgctgtcc agcagccgcc agccgcccag atttctgctt
    11281 gcggcatcga ttgccatgat ctcggcgtgt gccaggggag aacggtcgat ctcccggcgg
    11341 ttgtagcctt cgccgatgat gcgcccggtg gacttctgca ctaccactgc gcccaccgga
    11401 acttcgccca ttgccgccgc ctgtttcgcc aggtctaagg aacgcatcat ataccgtgcc
    11461 tgcggcacaa atccggtttc cgtcaaaaaa tcacctcgat caccatcaga acgatgcaga
    11521 gaaagaacac gacgctcagc ggtcccaggt gcatggtggt gtagatgcgt tctttcatgg
    11581 aaaggtagtg tttctgcgtc agcagtgcag gacttttccg ggtgggacga atggacagga
    11641 gacagagcag gataccgaca agcagtacca gaaacaggaa cagcggccgg tatgcctgga
    11701 gcgtctggtt cggccagatc tccatggcga acagtccgaa cagcataacg tgatacagca
    11761 tccggcagaa aatggacggg atcagagaac cgctccggag aacggcaatg ccggaaacga
    11821 tggagaatgt cagttccatc agaatcgttg tccagctgtg cgaaacgcac actgccagga
    11881 tcgccgtgag gatgatggcg taccagtcgc cgaactgccg cagtacgtga aggacttctc
    11941 cgtggagaaa cagctctttg cagatgctga ccgtcagcag ttcaaagacc agatagacca
    12001 tctgataaga cggccgcata taggatacgg tgtaatacgc ctcgcccagc gtgctgttgc
    12061 tgaggaagtt gatgggcgag aacagcatcc acacgttggt cacggcaaag ataatcagcg
    12121 tgaccgcaat ggtcgccggg aattcccgtg gggcatccgg tttcagatga aatgccacac
    12181 gccgtggcat ccggaaagtc agccggaaaa tcaagatcgg caccagatat ttcagaatgg
    12241 ttttcagcat cagaatggct aatacagcgg tctggttgcc gtagaccgtg ccgtcgctgt
    12301 agcagtatcc cacgtccagt ccgatgacct gcgccgcacc catcagaagt gcagtcagca
    12361 ggttttcaat cagcagaaag gcaaacagcg ccatgccgat caccatattg atctgcatga
    12421 tggcacgccg ctcttcggct gccgggtccg gttcgtgata gccctttccc tccacataaa
    12481 cagcctcttt ggtgcgcttt cggcagcgga acgcatattc gttcttctcg ctgctgcgcc
    12541 aggtacgaaa ggctttattg tatgcctcgg atgtggtacc atagttggac tctgccacca
    12601 catctaccgt gtccggggcg gatgccggta aattcttcat aggatttttt gtcatgttgt
    12661 tacgctcccc caagcgttgc ggcagtaccg cagatgttcg gcatctgatc gcaccctttt
    12721 tgcagaatca gtgcctgcgg cagctgctga aacagtagtt taccgaatgc cgagtgcatc
    12781 cgggaacagc agcatctttt ttctgtgtat tcagaaattt gcggctgtat atcaaattac
    12841 tgtgattttt ctcatttttt ctacttatat tatagcacac ccgaaaaatg gatggt
//
LOCUS       DAWENO010000049        12682 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2087_length_12682_cov_9.998891, whole genome shotgun sequence.
ACCESSION   DAWENO010000049 DAWENO010000000
VERSION     DAWENO010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12682
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2087_length_12682_cov_9.998891"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7065..7074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10907..10916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2500 a         3695 c         3481 g         2986 t
ORIGIN      
        1 tgtgcctttc cagcgcctgt gcctgcgccg ccagcgcttt ggcgttttcc gccaggaatg
       61 cgccggtatt ctcttggggt cttgtctcca tgtctgcggt ctcctttctg atgcagctgc
      121 aaatggattc gatgtactgg ctgccgtcct ctgccggaga caggcacagc agctgccgaa
      181 tctcctgtgc cagtttctcg ctgccgggca gttctccggt actgcgcagc agccattttt
      241 catagggcgc atatctgccg cagagcaggt gttttgcctg catggcggct ttgcagaact
      301 ctgccagata cagctgtgcc gtgggaatat cgccccggct gcgcatccgg gaatagttat
      361 actgtccgca ctgcgccatg cgtcccagcg cctgtgccag ccgccgcagc cgcacctctt
      421 ccggatagcc cagcgccagc ctgcggcgca cgccggaaaa tgctccggca tcgtcccgga
      481 acacagcgcc gctgcacgcc gccgccagca ttccgtcccc gacggcaagc cattcctgtt
      541 ccgtccacgg cacgccgggc gttcccagca gccgccggaa aaaggcactc tgccggcaca
      601 cgcccactct gccgtctgcc tcgggcattt ccgtccgggt gataccgtaa tagcttttag
      661 gcagggcggc ataggcgctt tgcagccgct gaactgtctc ttccgggaga tcgtccggca
      721 cccagataca gaaccctgcg ccgaagtcgt ggtcacggga cagggcatcg tcatagccca
      781 ggcactccga gccttcgccg gcgagtccca ccgccagccg gggcagaatg tccccgaaat
      841 ttcgttccag catgggtgcg ccgaaagcgt catagtactt ccggcagagt tccagcccag
      901 aaaacgccgg acggctgccg ctcagtctgg cataggtctg ggtcagattg tcggagacga
      961 tatcataaaa gttgttgcgc cccatgtgca gttcgatttc cggcagggct ttttcgtagg
     1021 catccgccgc cagttcaaac tttccttgct ggtaatacgc atcgccgaac ccggcgagga
     1081 cggcgctgta gtgaaaatcc gagggcgttt ttccggaaag tatgcggttc gcctgctcca
     1141 ggtaagagag cgcctcttcg gtccggtgca gccgaagcag ggacaccgcc agattcgccg
     1201 cagagatgcc agttcgggtg ggatcggggt cattctgcac cagttccagc gcccggcgca
     1261 gacagctgca cgcccgttcc cattgttccg tctcctggca gagcagcgcc aggttgttcc
     1321 agaggctggc aactctgccg tctgtgggcg gcagcaaagc gtcgtaaagc tgctgcacag
     1381 tctcaaaggc ggcaaaggca tcctggtact tcccggcagc acgataggcg ttggcgcagt
     1441 tcagcagggt ggtggcgtag gaaatgctgt gggtgtcccc ggcgttttcg aacagggcac
     1501 gggctttggc ggcggtgccc agcgcctggg gaaacttgcc gcagtcccgg taaaacccga
     1561 tctgctcgtt gcgcaggagc ttttcgcttt ccatgtcctg ctctgcctgc gcctgggcga
     1621 tggcttgggt aaggcagacc tctgcctcgt ccagtttgca ctggtgcagc agggtgtcca
     1681 gacgggaaag ggtttcctgt atttgcatgg gcggtctcct ttcttcggat ataacaaagt
     1741 gaacagaggg cagagtgtcc agtgttcact tgaggttggg tatttttgtt tggaaaatcc
     1801 aatataagcc tcctctccta gaggaggtgg caagccgcag gcttgacgga ggagtcacac
     1861 tgccagtcgt gcgatacatt gccttttcat aaaagggtag gcgaaaagct tttctttttt
     1921 tgcttctttt tttcttttca ggaaagaaaa aaagaagagt agcttttgaa atcccccttt
     1981 tcaagggaaa ctaaatactc ctctgctctg tcgggcatct cctctatgag aggagacaat
     2041 ttgcgtggca aacgctatga aaaaaacaaa aaccacattc aaaacaattg cctagaatac
     2101 caacgctgca cgtacaggaa aaggaagaca aaaggcgttt gcaagcctaa ggcggacagc
     2161 gagggaaacg agcgattccg ccggacgggt ttgaagcgtt atccctagga cagtgtttcc
     2221 cggaatagga aaagcgtccc gggcgaacaa tgttcgcccc tacgatgtgt tttcaaaaca
     2281 ataaggcggc gactcctctg ccctgtcggg catctcctct atgagaggag acaaattcgc
     2341 aaagcctcct ctcctagagg aggtggcaag ccgcaggctt gacgggggag tctcggacag
     2401 cccgggcgaa caatgttcgc ccctacggta tatgtaatgt tacaatagcg aaaagcagtg
     2461 tcagccggcg tttatgcctc ttccggcagg atggtgaaga aatgctgtgc caggtggtag
     2521 atcgcaccgc tgggcagttc ggtgtagccg gtcttttctg ccggcatatc cagattcggc
     2581 gcatcgatca ttgcggtcat cggctccggg cagaagtagt gtttttcgcc acggtcattc
     2641 cacagaatcc agaagtggta gccgtccgat acctcatagc cgataccctt tttgctgacg
     2701 atgtcagtca tcagtacgcc acggaacggc tttccacggt aactcagctt gccggcggtg
     2761 tacatatcgt tatccacaat gcgctttacc gggcaggtcg tgccgttcag ataagcctta
     2821 tcatagtcgc tcagcggcag gatctcgccg gtgggcagac agcgttcgtt cagcgtccac
     2881 ttctccacaa ccggcgcctg gatgcggata tcctcttcct ttgcgccgtc tacgaacggt
     2941 gcggaaatgg tggtgtgggt cgctacggaa atgggcagct gtttctgaga ctggttaatg
     3001 atggaaaact tgtattccag accgtattca ctcagcacaa agaaaaactc tgcggtgaac
     3061 ggcaggggca tatactggaa gaacgggtcg ctttcgttgt acatataagc agtccgcagc
     3121 catgcgccct cttcgttggc agagtatgcc actacggtgt gttcccgctt gtgcaggaat
     3181 ccgtgaatgt ggttgtggaa cggcgcagcc tcgttgaccg gcagctggta agtcgcatca
     3241 gaggttttca gcacaccgtc tgcaaaacgg ttcggcagat acagggtggg cagtccccag
     3301 acttcggcag actgcttgat gtcctccggg gtgttgttct cgtcccagcg gaaaaattcc
     3361 acgccgtgtg cgtcatcgtg gagacgcagc acgttggagc cgatgcccgg agcgatcagt
     3421 gcggtatagc ctccggcgca gagctggatg cactccatgc ccttccagtt gatcttggtt
     3481 gcggtattct tcataatggt ttgatccttt cctatgattt tccacagatg tggagaaatg
     3541 tattcatttt tcggcattct gacgaactgt gccgctgaga atgcgcatct ctttgttatt
     3601 atagcatact tttcgtggct tgtctataga aacagagaaa aatattgaga ttttcgggca
     3661 ccccccttga caaacctgct gcggtgtatt ataataaaat agaaagagag cctaccaagc
     3721 aagcggtatg ctcccactgt tttggttaag gaaataaccg ccacaaagta gcgagctgtg
     3781 ggcggttatt tctttttctt ttcatggtga tgctggttac tatgtttatg attatagtaa
     3841 atgtaagcga tcaaatttgc cagactcagc agaacactac agatctttag caaatcacca
     3901 aaggtgacat attgatccat cataccgatc acctcccatc tgctgcaaat gcacagtggg
     3961 agcagaaaca aaccgcctac cgttacgata agccctcaga ctctcagtat accacacaca
     4021 ccgatatttg tcaaatcaca caagcacacg aaaaaccgca ctgctgccgg gatcgttcct
     4081 gaaagcaatg cggttctttt tggttttccg cttactgtcg atttgtgcga tattgccttt
     4141 acttatcccc gtcaatatac ttccagcagc ccttgagata gaccagaccg gaaatgatgg
     4201 tcaggatggt ggagatccag atgagtccgc ccaggatcca cgttctcacg aaatccggca
     4261 cagccagacc gaagtcatac gccagagaca gatatgccat aacgcccacg atggtcacca
     4321 tggtgaaagc ggttttcagc ttgccccaga tgccggcggc gatgacaacg ccctcttctg
     4381 cagccagcag ccgcagaccg gagaccataa actctctggc aatgatgatg accagcggaa
     4441 tggcagacat ccggaattct ttcagttcca caaaaacggt cagcgccgtg atgaccagca
     4501 ccttatccgc caggggatcc aggaattttc cgaagttggt caccagccct ctggcacggg
     4561 cgatcttacc gtccagcata tccgtgagag atgccgccgc aaagatcacc agcgcccaca
     4621 aaaagtggag cggaatggcg tccacataga tcatcaccag aaaaaaaggt atgagtatca
     4681 cacgcatcag cgtcagttta ttcggcaggt tcatcggtta cctcctctgc cagcaggtcg
     4741 tagtccatgg tgtcgaaaac ttcaacggta atatactgtc ccagacgcag gcggctgtcg
     4801 ctcttgatga acaccttgcc gtcaatatcc ggcgcatcgg ctgcggttct gccgaagtag
     4861 catccggcgt acttgtcata gccctcgact acggcggtca tgcgcttgcc cagctttgcc
     4921 gcattcttgg cggcgctgac ttctgcctgc tgttccatga tgatatccgc acggtgcgcc
     4981 cggacatcct cgtcgatctg atcttcaaac tcggcagcct ttgtgccttc ctcttcggaa
     5041 taggcgaaac agcccaggtg atcgaactgc atatcctgaa cgaaatcgcc cagccggttg
     5101 aactgttcct tggtctcgcc ggggaatccg gtgataaggg tggtgcgcag ggtgatatcc
     5161 ggcacacgct cccggatgtg ggcgatggtt ttccgcaggg tctcctcatt gcccggacgg
     5221 tgcatccggc ggaggatctc tccgtcacag tgctggatgg gcagatccag atacggcacc
     5281 atgtgcggct cttccgccag cacatccagc agctcatcgg tgatgcgttc cggataacag
     5341 tacagcacac gcacccagcg gatgccgtcg atgtgacaca gctcccggag cagttccggc
     5401 agtctggact tgccgtacag gtcgcagccg taccgggtgg tatcctgtgc aattaccacc
     5461 agttctgtca cgtgatgctc tgccagccag tgtgcctctt ccagcacatc ctccatgggg
     5521 acgctgcggt acgggccacg gatggcaggg atggcgcagt aggtacagtt gttggaacag
     5581 ccctcggcga ttttcagata ggcgaaaaac ggcagggtgg tgataatgcg gtcacccttc
     5641 atttcggcat cgctcttctt gccgaaacgc accactcgct cgccggcgag aacgtggtcg
     5701 agaatatcca caatgtcccg gttgtcgccg atgccgatga cagcatctgc ctctgtgaac
     5761 agctccgccg cctcttcttt atagcgctcg gtgagacatc cggtgaggat gatctttttc
     5821 agcgtgccgt cttttttcag cgcacccagt tccaggatgg tctcaatggc ttcttctttg
     5881 gcggactgaa tgaatccgca ggtgttgaca atggcaattt ccgcctcgcc gggctctgtc
     5941 acaagctgat agccgtgcgc acgcagcttt gccagcatac gctcggaatc taccagattc
     6001 ttggaacatc ccagagagac catgcccaca cgaattggca tagttatttt cctccatgcg
     6061 attgtttttt atgatatgta tagcagtttt cgccacataa atcaattttc aaaaaatgaa
     6121 gtttgacaag tgaggctccc ctgaaagggc acccgaaggg cgtgccttgt gctgagctgg
     6181 cagtgcgcag cactgactga ggggtctcca cttatgccga aaacgctata aaaagatttt
     6241 acgaaaaacc tctccaccgc ctaacggcgg tccccctccc ctttcagggg aggctgattt
     6301 ttactgtgct gccgctgttt ccgtcgtagt ctctgcggca ggtgccgtgg tctccggctc
     6361 gggaacatcc gggtcgggcg gcagcttata gttgggctta gccgggtgca tattgaacac
     6421 gatcatgccg ttttccatct ccacgccccg gtaataggtc agttcgctga gtgtcaccag
     6481 ctggtagccc tgctgcacca gagacggcac gatctgcgcc acggcatctg ccgtttcgcc
     6541 gtagatgtcg tgcatcagca ccacgtctcc gtcctcgatg ttattcagcg ctgcactaac
     6601 ggtgcaggcg gtgttccggg tcttccagtc cttggtgtcc accgaccaga acgccagcgg
     6661 ctttcccaca tggcggcgca cgtcatcgtt gaagtccccg taaggcggtc tcagaaaata
     6721 gggatagcgc ccgatggctt tcagaatgtt ctcgtccgcc tgttgtatct gctgccggaa
     6781 tgtgtccgca tccgctttca ccaggtcagc gtgattctgg gtatggctgc cgatctcgca
     6841 gcccatcccc acggcacgct gcatcacagc gcctgtctcc tcgtcgatgt tctgccccac
     6901 cacaaaaaag gtagcgtgag cattgtatgc ggaaagggtg tccaggatgc tgttggtata
     6961 ctgtcccgga ccatcgtcaa aggtcagcgc caccatgggc ttgtttgggt cgatattggc
     7021 gctttcgtca ataaacggag cgcagagtcc cggcgcagtc gtagnnnnnn nnnnccgtgg
     7081 tctctgtggt ggatattgtt gtgttgtctg ccgcagacgc cgccgccatg accgcctcat
     7141 gggtctgtct ggcattggct gccggcgcat tgatcttggt gcaggcgccg cccagaacaa
     7201 acagggcagc cagcacagca catttcagca tacgctgtct ctgacgcatt ttttacacct
     7261 ccgtatcgga cagcactgcg caaaatccgt ctgacggttt tgcacggtgt tgccataggt
     7321 atttcttttt cccctattcc tcttccagct gttcctccag gaatgcccgg acagctgcgt
     7381 tgatctcacc gaaccgatag ccctggcgca ccagtgccgc cttgcccttt tcaatggtgc
     7441 ggcggtcgtc cgggtcgaga aaatacttcc ggtattttcc ctccaccagt tccatcagga
     7501 tctcgccggt gacttcgtcc tcatacggtt ccagcgcctc ggtgatatgc tcttccagca
     7561 gtccccggcg gcgcatctcc tgaaacgccc ggcgcatccc gaaccgcttt acggcaacgt
     7621 aatgatgcgc cagctgttcc gcataggcac ggtcgttgat ctgcccgtat ttcgccaggc
     7681 gatccaccgt ggcgaaacac accgcctggg gatacttcgg ctccagcttg cggaacattt
     7741 cctgatagct gtagccccgg ttgtccagca gaaagcaggc gtactggtat gccttccggg
     7801 aaagctctgc ggtctgcacc tgttcccact gctgcggtgt cagcgtcatg ccctttcgga
     7861 ggccgcaccg ctgcaccacg gaaacatgga gaaagaacag ctctcccgaa tcgtccagtt
     7921 cgatcttcca ggtattgccc ttatgggcgc tgagtgtggt aatggtcatc tgccgtcacc
     7981 ttatgccgga tcgaattcct cgaagtcgtc ctcggcactg atgtccaggc tctcgccctc
     8041 ttccagagag ttaaactcgc cgtcgccctc tacctcaacg atatcgccgg gttcagcgtc
     8101 ctctccgccg gctgcctttg ccgcagcctc ttccagcttg gacttcttct ttttcttctt
     8161 ggacgttgcc atcagctcgt ccttatgttc catgatcttg tcctcgatct ccttcatcag
     8221 atcggggttc tcttccagag tcttcttggc attttctctg ccctgtccca gcttctgatc
     8281 ctcatagctg aaccatgcgc cgcctttttt cacgatgccc agttccaccg ccacgtcgat
     8341 gacctcgccg gtgcgggaga tgccctgtcc atagaggatg tcaaattctg cctcacggaa
     8401 cggcggcgcc accttattct tgttgatctt caccttagtg cggttaccga tctggctgct
     8461 gccgtccttg atgggttcgc cacggcgcac ttccatgcgg acagaggcgt agaacttcag
     8521 cgcacggccg ccgggcgttg tctccgggtt gccgtacata acgccgatct tttcccggac
     8581 ctggttgatg aagattgcca cgcacttgga cttggagatg acggcagtca gctttcgcat
     8641 tgcctgggac atcagtctcg ccagctgtcc cacgtggctg tcacccattt cgccgtcgat
     8701 ctccgctttg gtggtgagag ctgccacaga gtccacgacc agcacgtcga tggcaccgga
     8761 gcgcaccagc gcctcggcga tttccagcgc ctgctcaccg ctgtccggct gggacaccag
     8821 caggctgtcg atgttgacgc ccagcgcctc ggcataggtg gggtcgaggg cgtgttccac
     8881 gtcgatgaat gccacttcgc cgcccatctt ctgcgcctcg gcaatgatgt gcagggcaac
     8941 ggtggtctta ccggagcttt ccggaccgta cagctccacg atacgtcccc gtggcacgcc
     9001 gccgatgccc agcgccatgt ccagcatcat cgagccggta gggatcgcct ctacattgag
     9061 gttcgcctta ttctcgccca gacgcatgat cgcacctgtg ccgtatacct ttgtaatatg
     9121 tgcaatggca ctctggagtg cctttttctt ttcttcctcg gtggtgggga tgtccagcac
     9181 cactttcttt ttcagatctg cttttgccat aaaatatcgt cctttatctg gtatggatgt
     9241 atcaacttac cgggcatttg cgggcgagcc atattcgtct gggactccga ccgcactgtg
     9301 tgccgccttc ctctgtaaga gaaggctata tttcagcttt ctgccccagc accactcgtc
     9361 caatgcctgt gaggtcgggg acggtgtgta cctgggcata gccgtttttc cgaaagatct
     9421 gttccacatc tgccgcctgt cctgcgccta cctcatacac cagccagccg cccggcagga
     9481 gcgccggttt ccagagcatg gtcagctttc ggtagaagtc cagcccgtcc gtgccgccga
     9541 acaaggcggt ctccggttcg tgggcgactt ccggctgaag ctgttccata tcagctgcgg
     9601 tgaggtaagg cggattacag gtgatgatgt gatactgctg ttccagcagc gccgccggcg
     9661 gacacagcac atcgccggca cagcattgca gctggggtgc gtggcggtgc agattctgcc
     9721 gcagatacgg cagggcatcc ggcgacagtt ccagcactgc cccggtgaca tccggcagat
     9781 gcgccgatac cgccgccggg atacagcccg aaccgctgca aagatccagc agacaggtag
     9841 acggcgcacc ctttcgcagt tccagcaccg tttccaccag ggtctccgta tcctgccgtg
     9901 ggatgaggac gccctcgccc acccgaaatt ccagcccgaa aaattcccat gtgccgcaga
     9961 gatactggag cggctcatgg tcacagcggc gtgcggtcag ttccgccagg cgctgctgct
    10021 gggtttcggt cagaggctgt tccgggagcg gtgcgccata gggcagttcc agcaccgttt
    10081 ccaccaggat gcggctctcg aactgcgccg attccagtcc ggcgttttcc agccgccgtc
    10141 gggtctcctg cacggcggca cggggcatgt ttaccacttg tcgtcctcca tgtcctccgg
    10201 gatgcagggt ttcagcgcag cgatggcgca ctcgatctgg gaggcatccg gttcacgggt
    10261 ggtgatgcgc tggatctgaa gtcccggcca ggaaagcgcc cgggtcagcc agttgttgct
    10321 ccgtccggcg atgcggatga actcatagga gatgcccacc accaccggca gcaggagaat
    10381 gctgacgatc atccggggaa cgacctggta gatcggcaca atgcacatca ccaggatgct
    10441 gacgatcagc gtgataaaga taaagctggt gccgcagcgg gggtggaaac gagtgtgttt
    10501 cttcacgttc tccacggtca gctcttcccc tgcctcatag caggcgatgg tcttatgctc
    10561 tgcgccgtgg tattcatagg tacggcggat atccttcatc atgccggtac acgccatata
    10621 ccccaggaaa atagcgatct tgatgatgcc ctccagcagc gaccgcacaa agcggctgtt
    10681 gtccgggatg gcggtgtggg cgatgagctg tccgatgagg aacttcggca ggaacacaaa
    10741 cagaccgatg gcgactgcaa tgcccaccac catgccgatg gacatgatga tgccgaaaag
    10801 cttgtctcca agcttttcat ccagccactt ttcaaagcgg ttcatctcct cggtgtccta
    10861 ttcgtcctca tcctccggca cctgtttttc cgccgacttg gtcaggnnnn nnnnnnccgt
    10921 ccttcatgga cagcacaaag ttaaagctgc cccgaagaaa cgggcatttc cggtaccagg
    10981 gcatattctt gccgttgttt tctttccagg tctccacgtc gatctcgccg ttggggagac
    11041 ggcacgccat agcgccggtg tcagcgcctt tcatcataac gccctcgatc agcgcctgtc
    11101 cgccgatctt ggtcatatgt ttcgggtttt ctctgggttg ttttgccatg tgttgtcctt
    11161 tcttctggat aaacaagaac gcctccggtg catcatagcc cgaaaggttc tgtttgcatt
    11221 atgttgcggc actgctgcgg cgctgcgcct ctaccgggag ggtgatggtc acgttggttc
    11281 ccacgttcag ttcgctggtg atatccagtc tgccgccgtg catggtgatg atctcatctg
    11341 ccacagcaag cccgatgccc gatccacgcc gggtatggtt cgccttgaaa aacttggtct
    11401 tgacacgagg caggtcagcg gcgctgatgc ccacgccgtt atcggtgata gtcacctgaa
    11461 tcatgtttcc cacttcctcg gcgctcaccg tgatggtgct gccagggttg gaatacttga
    11521 tggcgttgtc aatgacgttg atgaacacct gccggatgcg gttcttgtcg ccgtagacga
    11581 agggcagcat ttccggctcc tggtagatga tatggatatt ctcccgcttt gccttttccg
    11641 tatagatcag caccgcatcg cccagctccg ccagcacatc catagtggcg cactggagcg
    11701 agaaccgtcc gctctgcatc cgggaaaagt ccagcagctc ctccaccatt tccgacaggc
    11761 gctcggtctc gttcacgatg acgtggacgc cacgctgtac ggtgtccggg tctgcctcca
    11821 tgcacatggt ctccgcccag cccttgatgg cggtgagcgg cgtgcgcagt tcgtgggaaa
    11881 cggaagagat aaactcgttt ttcatgttct ccgtattgga gagttcgtcc gccatctggt
    11941 tgatggacac gcagagatcg ccgatctcgt cgctgctgtt ctcgctgatg cggacagaaa
    12001 agtccccttt ggcgtactgc tttgtggtgg cgttgatctg cttgatgggt cggacgatgg
    12061 agcggatgaa atagagtccc gtcaccacca gcagcagcag cactccggca cagactgccg
    12121 aaacgccgaa gatataggcg tttacggtct cctggatctg atccagagat gccaccaccc
    12181 ggatggcgtt atactccgtg ttcatgtcgg agatatccac ggagagcgcc atgatggtct
    12241 cgccgccctg cttgcccacc caatagccgt cgccgccgtc catggcagat tcatagtcgg
    12301 gcatggctgc gccctcttcc ggagtgaacc cggaggacgt gagcgccact ctgccacggg
    12361 aactgatcgc cataagttcc atcttgtcct tgtcggagaa gttctcgatg gtgctgcgca
    12421 tttccgagga aaagttgatg tcggcgtcct gggaatagcg gctgaggaca ccggtcacgg
    12481 aggtgagctt ggtgttcaga tactgctggg atgcgctgta gtaatagttc tgcatgatgt
    12541 agatgaatgc aaaatccagc accgtcagca ccagggcgat gatccccagg ttattgatga
    12601 cccagcggcg tgtgatcgag cctccggcac gtttttttgt gccatgtctg cgtttatttg
    12661 tcattccact tatatccata gc
//
LOCUS       DAWENO010000050        12221 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2197_length_12221_cov_9.869061, whole genome shotgun sequence.
ACCESSION   DAWENO010000050 DAWENO010000000
VERSION     DAWENO010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12221)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2197_length_12221_cov_9.869061"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3605 a         2923 c         3172 g         2521 t
ORIGIN      
        1 aataagagtc tgactgtgcc gaataaggca aacgatctgt actacggttt cctgctggag
       61 aatggggcaa cttcgggctg ctactccaag gcagggaagt cgctggtttc cgttgacctc
      121 cagagcgtag gcgtgaaaac agaaaaggac agcgccaaga tcgtaaaggg ctacctgtcg
      181 gacaaggtga cgatctcttc caactatgcc aagagcggcg tgatcgaaaa cgtcaagctg
      241 accgcattcc tgccggatgg cattgctggt cctaaacagg tgaaaacctt taccctggaa
      301 gaactggaaa agaacaagaa tgcggcaggg gaactggaaa tggacctgtc cgattggaaa
      361 gcacgggacg tataccttgc gaatatcgaa atcgcttatg ctgatctgga gcgtgacctc
      421 tctgcggagc agggcaatgc accggagctg cgcatctacg gtacaagcga ctggttcgat
      481 gatctgacgg cgaaaatgac agtgacaccg cagcatgagc tgatgcagaa tcaggcaaag
      541 tctgcgtctg tgacattcca cgtagaccgc ccgggactca gcgtcaacac acacatttat
      601 tacaacgatc agaaagaatc tacacgtgcg gcatcgggca atacggatgg aaatgaaacc
      661 attttcggcg ttccctatga ccgtgatttc atgctgcgtg cagagtttgc caatgagacc
      721 atttcggttc tggatgacac ccagatcatt gcgaagatcc catatgaaaa gcagggagaa
      781 gaagaaaccg gtttccataa gacagaactg cgtatttacc aggatctgat cgaccagttc
      841 ggtgcatttg acaatatcat tctgactggt aaggatgcgg ataacctgga gaaaaccgta
      901 actctgctgc cgaaaaccga cgagaacgga aagctcaccg gtttctatac aaaggatgcg
      961 ccggatactg tttatactta tacagacggt gcgctgacat tcacccgaga cacagacaca
     1021 cggctgttcc cggcgttcgg tatcaagaat ctgacacagg tggaactgaa cggcagattg
     1081 gtaaaactgg tggacaagac cgcatcggaa aagcggtata tcgaatttga cggttatgac
     1141 gattccctgt tcggaaagac cgatacactg gaagtggaga cacacaacta tctggacggt
     1201 ttccgggaat ccaagggcta tttcacagaa ccatacgata aggacaagta tatcgtcacg
     1261 aagaaagaca ccacagcgat ctatgtttcc aagatgtatt tcgacaccac cgtaatggca
     1321 ttttataaag atgccgatgc cggagacaag aagtacacgg cggcagccag cgcagtggag
     1381 catttgcgga agaagtatgc ccagcccaca gaaaatatga cgacttgctc ctggagaagc
     1441 gataagcata tacacggcgc agacggttat ctgttctctg aaaaagagaa gtggggagat
     1501 agtgcggata actccgagtt ggagatcggc tataaatcca tcggttcttt cgctgtggac
     1561 ttccgccagt atctgaacac agggaacaat ctgcccaagg caaatccgac ccttgccgga
     1621 caggaacacc agtcgtatga ctacgttcgc aatgcgtctc tgaatacggc ggcaactgtg
     1681 gatattacat tggatctgcc gtcgaatact ttcgatgcgt actatctgaa aattgatcct
     1741 cgtgccaagg actatatcaa atccgttacg gcaatctatg cagacggtac caagcgcatc
     1801 tgttccggac aggaaattct ctatgaagcc aacgagagca atacaaacgg aaactttgca
     1861 cggctgaatc tgctgggcag tgatgcggat atgttctctg aggacgattc caactattat
     1921 aaaaagccgg agagctatga cggaaaagaa ccggaaaatc cggtaaaaca ggtagttgtg
     1981 accatcgaca tcaaccgcta ccagactgtc aataatgacg gcaaaacggc aaagtcgccg
     2041 gactacggca catggtatga ccagacagat tcttctacac agggaatgtt cgaggttacc
     2101 ggacgtttct accgtatgga cgaggcagtt gccaaagcac acgcagatgt gaccgtgggc
     2161 aacaccactg acgacagaag taaggtgcgg acagattcgg gtgtggacaa ggaaaagtcg
     2221 gaatggtctt tcacagatca gtattatgcc tggacaccga atcatagcta tagtcatggt
     2281 cattactgga cttatgagat attcgactat actgcaaagc atttgcagtc cagcacacgt
     2341 gtgcatattg tcaatgacca ggacaacgta cagaagggtg tcggcgaaac acccggaacg
     2401 gcagaaaatc gcaatgcaga atttggacag gacaatagtt ttgcaattcg gttttatcgg
     2461 cagggcagaa ccacaagtgt tcccacctct aaagtgaatg caggatatag tacatctagc
     2521 cgttctgcac cgaacaataa cgaatatcat tggacaatga gcagcccgta tgttcgtacc
     2581 gaccagtacg actgggtgaa caaactttcc tttacggata aggttgtgct gtcggacagc
     2641 ctgccgcaga ttcgtccgga tgatgactat gaatacaagg gctttctgac tacgggcatt
     2701 cagatcaaga aggagatcta tcagtatttc cgggatgacg acaacatcat tttcaccata
     2761 aatgcaaaga acacgaataa tgacgttgtc gggtctgcac agaccatttc tgttccggtg
     2821 tctgaactga gaaagatgta cggcgaaact gaggactgga acatctattt cgagtacacg
     2881 ccaagacagc aggacaatac agatagcgga gacgatggag acggcagtac cgaagaagat
     2941 gcggatgatg cggacaccgg tgcagacgag ccgcagatcg aaccgaccta taccttaaaa
     3001 acagacgacg gcaagtatgt ggtagttctg ggcgaagata cctttgtgac ggactacagc
     3061 atgactatgt atgacattcc gggtacaggg gattattctg cggaactgac cgacaaggaa
     3121 aaggcagatt atcacaacca gaatgcctgg gatattctgg tacagggcaa gccgtatgcc
     3181 atcaacaaga cagataagga tatgtacggc gaaaatactg ccaagaccag cgtttccacg
     3241 gattatgacc cggatgagaa aatctacagc cagtcggata atgccaccat catgggctat
     3301 ctgatcgact tcaaggctga ctatgcgctg caaacaaaga gcggcaccac atcggaaaag
     3361 ctgtactacg attatgacca gacagacaac aacacaccga atcgtggtct gttccaggtg
     3421 aaactgttca accaggacaa gcagaatgcg accggacagg atgcggcggc acgcattaac
     3481 agcgccgcta ctaccaacac catggacggc agctatcggt tggagcatat ctacctgccg
     3541 gcattcctcc tggatgggga ttggttcggc gtggagaatc tgacgctccg tgtgggcagt
     3601 acggacgttc cggtgaacgt ggtcaaaggc attggcgtag acggtatggc ggacggtatc
     3661 tatgtggttc acgttgatac aacaaagaac gtacagggcg aagaagttta taccattgat
     3721 gtggaacaga ttctgcgcca gctgtatcag gcaaattcca gcgttctcgg aacttatacc
     3781 aattccacca accagaaaac ttatgtcaag gcatatgtca gcagtttcca gctggatctc
     3841 aaggcgaaaa aggcagaccg aaatgacagc agtactgtgc tgggcgacgg ggaatacctg
     3901 tccgcaagca aaagcaagga caagagcgca ttcctgtatg acggcgttta tgtagaccgt
     3961 acaccggacg atttcaccaa ggatgactgg aattccaact caacgccgtc ctttggaaaa
     4021 tcccccaacg gctatccgga gcagacaccc aagaaccagc tttcggcgac attcacatcg
     4081 gttgacccga atgccaatac ctatgtttat aagaacacag acggtacggc aaagggcagc
     4141 aagaaagagg cgtcctatac cgtcaagaat ctgaccggca cgctggatgc ggtagttact
     4201 cgcaccagga tgtacgacca ggattccaag accggcaaca tcgataaggt ggactatctc
     4261 catctgacgc cgtatgacta cgtagagtat accatccagg tgggtgcgcc gagcaacgca
     4321 ctgattccgc tggagcatac cacagtggac ttctctgttc ccaagggaca gcggatcgtt
     4381 cgctgggatg tggtagatgc aaatggaaac atcaagacag acggcaacgc tgcagagaac
     4441 attccggctg cggacatcca cgccacactg tcagacggcg gcacaacgat cgaggcggca
     4501 cagggtgtca agtacgcact gcttgccaag tcggatgcag acccggaaga aaccgcatac
     4561 cagcggctgg tggctgaact gggcaagggc gcaaaatccg atgaccagat caagccgggt
     4621 gaaacggttt acctgcgtgt gaccacacag ctgacagaaa acggcggcag ctatgataat
     4681 gtgactgtcg gcacgccgga tctcaagatc catgccgcac ccaagcacgg ctatccgcag
     4741 tattctattt atgatacggc aaatggtacg aattatacgc cgaatacaga caggcagaat
     4801 tatcgttgga acaagagagt aaaagacgat gccggatatt cgtattacta tcgttatacc
     4861 gaggctgatg cagacaaaca gacccagtat tatgcacacg tgcagaatga actgacctat
     4921 cagtctgttg cgccggtaaa gcttacctac caggtaaacg atgccaagca gaattacgac
     4981 ggcaagggtg ctgtgctgac ggtcagcgac ctgtacaacg agaccggaca ctactgcgac
     5041 acctatacca tagacgtttc cttcctggaa aagcgttcca aggacagcgg cgatgtgttc
     5101 taccgaggct ttgaactgac gaaaccctac acagttacag acaagttcgg cagagtggaa
     5161 atgctctatg ccgttgcaga gccgagtaca aacccacggt ttgcagagga tgaaaacatt
     5221 gcattcaaga cggatgagaa cggcgtaaag gtgacctgga agtcctatga atatgacccg
     5281 tccatcacta cagaccaggc agcgacagcg aaaatgacaa acgacgacct gccgaatgtg
     5341 gttggcgttc gctgggtgta ctatgacctc aggggctttg acaccacaac tgcgtacagc
     5401 agcaccacca agacaaaggt cgctttgcaa aatgtgacgc tgaccggtgt cggcagatac
     5461 caggatgtca cagacggcac aggcgtgaaa gcggatacct ataagcagta tttcacagcc
     5521 accaacacct atgaacacat ccacagcgaa aacgaaaacg cagtgacagt gaccgaaagc
     5581 ggcagcagtg tagcatctac agcaacacct accgaacaca cggtgaaact gacggacaaa
     5641 acagttctga accccagcgt ttaccgggaa aaccccattg catcgttcca cacccagaca
     5701 ttttcttccg agagcgacgc atcggctgcg tatgacgaaa agaaaacgca aaagacaagc
     5761 tatcgtcctg gagacaccat ctggcagaaa gtgacactga aaaacaacct ggcggcgctg
     5821 agtagcggca aacaggcagg cgaggagggc agactcatta acccggtcat ctatgataag
     5881 gtgccggaat attttgccaa gactttgtac gattcgtatg gggtcggcgc tagcataccc
     5941 aattttcatt ttcatttgcg tttgctgaat gcaaacggca gcgagaaaga tttgagcaat
     6001 attgagctgt atctggcaaa caaaaccgca caaaatgggt atgactacgg cggaaaaatg
     6061 acctatacag acgggttcaa gtcaaccaat gcaacacaaa aggcatttaa cgatctgaac
     6121 ccggacgaga attccacata tcagatttcc tttaccgtgt atgaactgcg cctgcgttat
     6181 aaagatgctc cggatgcgga cttcgttttg cagccgggtg agcagatcga gttctgctac
     6241 tcggcgacca ttcaggaaaa agacctgccc atggtgtaca ctgcgtccac ctatgcaaat
     6301 gatactgccg catccactgt ggacggcagc ggactgcatc cggcatattt cccccgcatt
     6361 ggtgagtatt atcagcagcg tattaaccac tattattcct gggatactac atatggcaaa
     6421 ctctatccta tgctgacgaa tggctttact agtgaaggaa atacccggaa caatttcaag
     6481 caggtacaga acagcaatat ccagatggat atggactatc tgatgcacga tattggtttc
     6541 agtgcggaca agaataccca ggtggatatt tgggaattcc tggatcagac cattacccac
     6601 attccgggtt ctggttccac agacgctacc attggcggtc agaatgacac actgctggat
     6661 gaggactccc agactgcaaa gaatatgcag acggtgaagt ataatgcaag ctccaaaatt
     6721 gatactgccg accagctgtc gcagctgccg cagacggtga agtacatggc aggcggtgca
     6781 aaccgagatt ggttccggct ggcaatgggc aagcggaaca aggactatgt agactgggca
     6841 aatctggatg ctgcaaagaa tacaccggtt gtatggagcg agacacgtct gcacctgcaa
     6901 aaggcatggc ttgccaccag ctcggaattt gtgacaagca atatccagta cgaaaagaca
     6961 aacggcgacc gcaccaataa tgttttggct gacccgaatt atccgtatta tggaacatat
     7021 tccgacggtc atggagtgaa ttcctattac aatagtgtac atgaggtgac agaccgctct
     7081 ggtgcggctg cgctggaata tgatgaagat gtcaaggtac agcttcaggc gtacaactac
     7141 ggcgactggg atctggacgg tgtgacattc ctgtatacct atgcctatgg catgaaaccg
     7201 gtgatgaatg cagacgggac gctggataca tctgagatac tggcgtacac caacaccggc
     7261 gactcgaaag caaatacatt ctcccagatc gacgggacat atgttaccgc agagatcatt
     7321 cagaaaccgg gagacaaaaa tccgcagtat cttgcaccaa aggcaatgcg ggatgcggca
     7381 cagaacgcaa acatcacaga cggcgcagac ggatattaca atgcagacga gtatactccg
     7441 tatgtggtga agatcactgt aaagcagccg ctgaaaaagt ggtacggcag aggcagtgat
     7501 ttcggctata tcacacgggt gacacttccg gcgagagtat acaccgatac ggcaagcggc
     7561 acatggtacg accgtgttct cacccagccc tatgtggcag aaaacagcca gaatcatctg
     7621 tactatcaga tctatgacat cgaccactgg gacggttcca ccaaggcgct gaccaagcac
     7681 aaccagctgt acggtatgga ctatctctgg agtccgctgt atttccattg tgatacggca
     7741 aatgaacagc ttgatagtct gaaatatctg gcatcctctc cgaatatgcc gtcagtaaac
     7801 ggctacaata tccagaacaa ggaagtcgta gcagacgaaa acggctatca ctttaccaac
     7861 aatgacaaca tcgatcgtta cagcagcggc acgaccatgt tctttgccga aactggtaca
     7921 agagccgtgc agcgaaagcc tttggtacgc agctgggcaa ccatcggcaa ggatgaaacc
     7981 gagatgtacg gcaagaaggt agagcagtac tacaccgaga cacagggtga ggtcaactgg
     8041 atcaacgtcc atgtggagaa caactattac tgggatgagt acggctacag cggcagtgcc
     8101 ggtactgaag ggtacaacaa cgaaaaatcg ctgcacagct atggtacaga cggcggtcag
     8161 aagggttcgc tctcgctgcc ggtcatcacc aacattctgc cctacggcat cgttccggtt
     8221 gcggcggatg gctctcgcta taccacggac aacgacaaaa acaacaagcc tctgaactgg
     8281 gagctgtacg acaaggatgc taccactaag ctgacagatc aaatggcact ctatgatact
     8341 gaggtctact atgatgattc taccaagcgg tatgtggtgc agattttcgc caaggaagaa
     8401 aacgacacag aaaaggcaat gacagcggct gaactggcag aaaagcagaa aaaggatgcc
     8461 gcaattcgca acggcagtct gtacaatttc tgcatcaaga cctttaccta tgcagagccg
     8521 gacgatgtga taaacggcgt ggaagaggaa gacctgctga aaaactatct gaataactat
     8581 tcttatgttt ccagcagagt gaatgcttac cggtttatca cggacaatga catttccgga
     8641 aatccgtaca aggtttgctc ggcggcagtg attacaccaa ttatcaatta ctattccacc
     8701 tatgcgttcc gaaaaaaaga cgaacgaaaa gatgcagttg ctgcaactgc aaaggtgact
     8761 cgtcattatt atggcggtgt ttattattac aaatccgaag ataccctgcc catgtgtccg
     8821 gacacagata acgacggact ttcgaccacc attccggatt cctatgttat tttgaaacat
     8881 aagtttgaaa aagacggcag ctatatgctg ggcagcatga gccggtatgc cgagagcgat
     8941 tccatggaaa tggacgaata ccggaaggac aagccgctgt atgaacgcat ccgttctgca
     9001 ctgaaccggg atgtgacact gcgtgacaac ggcagcgata tgactttcca ggatatcact
     9061 tctgcaaata tcatacagga tgccggcgtt gtgaacacgc tgaagatccg cactcagaca
     9121 ccgtctttga aggcggagaa catggtggct gcggaaaagg cagatacagg tctcaagggc
     9181 tttaccggag atacagagaa tctggacgaa gtgaaaactc gtcagaagta tcagatcctc
     9241 tggtacagcg ccaaggtttc cagccagtcc gaggcggatt accgctatcg tggcagtatt
     9301 ttccacgcca agatggtggt gacattccag ctgccggaaa atgtacggta ctgggatgag
     9361 gacgacctaa tggacgacta ctatctggaa tacacagata agtctggcgc caagcagaca
     9421 ctgaccatgg cgcaggcaag ggatgacggc tggggcattg agctgacaca acggtacttt
     9481 gctggagatg ttgcaccggc agaaaataca acagacggac tgacacgggc gtactctgcc
     9541 accaagcgtg gcggcgagac gctggtgttc gagattacta cgccggcaga aaaaggtttc
     9601 aactgtaagg atgacaagac ctatgttgca ggttatcacc cggcaggcta tttcaacggc
     9661 acgctgaact ttaagatcaa gacccggatc gacaacctgg aactgcaaaa cgacacagac
     9721 agcagctggg aggactatta ctccaaggtc tatgtaacct tcgaacagac agacggcaag
     9781 tttgccatca caaaggatgg tctgtttgca gaacgggacg cagacggcaa gtactacact
     9841 cgtggcggct gtctcatgcc gaaacaggac gaaaacggct atgtctataa cgactacggc
     9901 aatatcgtct atgagaagaa acagcggcag gaagttccgg caagtgatat cgtgtatgat
     9961 acctatgact tcaacttcac cgggcagacc atggagggaa tgcagctgca aaatgcagtg
    10021 cgtgtgaagg atgaaaatgg cacaaaggac agcacaatta aggagatcga accctttgac
    10081 ggcgttgtgc cgggtgagat ctatctgatc gcatcctctg caaagatcgc tgttataaag
    10141 ccggatacca cggtgcgtgc ggatctgaac tatgagcgtg tgcgtgtcaa tgacccgaac
    10201 tcctctatca ccatcgcaga agatccccat atcaagcagg tctataagac ggtcagctac
    10261 atcgaccagg tgtctaatga cactgcatcg gtaaacgaat ttgtggtgaa ctataacctg
    10321 gcgaactatg cgacactgcg tggtacgacc aaggttgcgc cggtcaataa gccggatgtt
    10381 ctgccgcacg tttccgagct ggtttatccg aatgtgacgg agatctctac cggtaactgg
    10441 gcgattccgt cggcagatgc gctggcaacc gaatatgcta aatatgcaga ccaggtgcat
    10501 gacattctgg aaaatcatct gaaagtgtat ccgtatgtac ttatcagtac ggaccctgcc
    10561 aatgaagcag aattccttgc accggagacg gagcaggatg acggtatcta ttccaacgct
    10621 tatgcaaaca acggcaaggg aaccagctgg tgggtacgca tcggtaatga aaatggttac
    10681 gcactggatg caaaccagac catttcgctg gagaccggca aactgcattt tgacagtacc
    10741 aatgagcagc tgaaaaaaga aatcacatcc ggcaagatca atctgaacaa atccattcgc
    10801 caggtgcgct gggtcatccg gacagagggc ttcacggaga caatttccgg aacccaggtc
    10861 gaactgaccg acgcagaggc accccggtat ttcccggtgc cgaaggggct gtatatgaat
    10921 gcagacgcag acgatgcaga agagggcaga caggaccgca atgatgtgga caaggatcac
    10981 tatggtctgg tatctctgag cgagagtatc aaggaaaaca gtcctcatgt tgcgtttacc
    11041 actgcgccga atgatacatc aattgcaaac tctgtacaca acaaccattt cgcaagcgtg
    11101 atcctgcgca gcgatgactt taagtatgcg gtttccggag aagtctccag agtgggcttt
    11161 tatctggatc cgcagctgcc gtggctgggc attgatattg atgccaagta tttcagcggc
    11221 gctgcaaaga cagcatacaa gtgggattcc aataccagcg gtatcagcag cagtactggc
    11281 aagatgctga agtatacgat tacgtataag aatctcagcc aggagctgtt ggaaacaact
    11341 acgtataacc gaaagggtgc ggaatccgat aagctgtcgg aaccgacggt ttctctggca
    11401 ctgccgtacc tggagtctct ctctgcggat aatttccagt atgtgaacta tcaggatgat
    11461 tccaatggca gcattgacgg ctatttcgtc ggcgatcact ataccagcac tgcggaaaag
    11521 aacagcgaca accaagtggt aacgccagca aagaatctgg atgatgtgac gccgcagtgg
    11581 acatggcata ttgaaaatga aaagggagaa tttgtttctc tgaaagaagc cggtgatgtt
    11641 tcactggctg atgtagatgt agagacaaaa acctatgcag acgtaaaaca aaccgatgtt
    11701 gccatggatg aggaaaagaa tcccaacccc tactttgtga caaaaccgat ctccaccaac
    11761 gtactgaacc ggaagatcgt caacttcaag ttctccggaa cactggagcc gggtcagcgc
    11821 attgtagtag aactgatggt gccgctggat atcagcaaca acaacatcgt gtctacggaa
    11881 ctgatgcagg caaaggcata cggattcaag aacggttcat tccggccgta tattccggac
    11941 gaccagggca agacacagaa aatggttgct tacgaagtag acagccatga tgtcaacgac
    12001 aacggcagca gcggtgatat gacactgacg atcacatcac aggcgattgc tctgcttgcc
    12061 attgataatg tcacacaaat gaaaacatcg acttctgatc tggattccgt gaaagcggat
    12121 gttccggttc gtgtacagga aggcggcaac tattcgtact ctgcctctat gcagaatgcc
    12181 tcgaccagca atgcagaata tacaacgctg ctgttctatg a
//
LOCUS       DAWENO010000051        11866 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2286_length_11866_cov_13.040894, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000051 DAWENO010000000
VERSION     DAWENO010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11866)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_11866_cov_13.040894"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2690 a         3388 c         2935 g         2853 t
ORIGIN      
        1 tagtgaaaca acaaagctta ccactacttt cacaagttca actaccagta caacaacaaa
       61 gcctaccaca acttctacaa gcttgaccaa cagtacgaca accacgaaac ttaccacaac
      121 ttcaacaagt ttaaccacta gcacaacaac gacaaagcct accacaactt ctgtaagttc
      181 aactaccagt acaacaacaa aactcactac gacttctaca agttcgacta ccagtgcaac
      241 aaccacaaaa cctactacga cttcggcaag ttcagctacc aacacgacaa cgacaaagac
      301 taccacaact tctacaaatt taaccaccag tacaacacct acaaaaccta ctacttccac
      361 aagtttgacc actagcacaa caacgacaaa gcctgccaca acttctgtaa gttcaactac
      421 cagcacaaca accacaaaac ctaccacaac ttccacaagc ttgaccacta gcacaacaac
      481 tacaaaacct accacaactt ccacaagctt gaccactagt acaacaacaa cgaaacctac
      541 cacgacttcg gcaagctcaa ccactagcac gtcaacgaca aaatctacta cgacttcaac
      601 aagttcgacc accaccaaca caaccacaaa ctccaccgcc acgtccacaa cagctgcaac
      661 aaccaccaca ctcccggaag aatggccaaa actgataggc gatgccgacc gcaacggcag
      721 agtggacacc acagacattt acctggtcat gctgtactgc gcccaccacg gtgccggtca
      781 tgcggactac acctttacgg aatatcccga actggaaaac gctctgctgg aaaagctgga
      841 tgttgaccac aacggtaagc tggattccac agatgttttt ctcatgatgt actatgttgc
      901 ctgcaacggc gccggcattc cggttcactg ggaggatttg atataacttt ttcctatttc
      961 caatataatt tacagtataa aaataaaata tagtatatat agaaacgatt tattattaca
     1021 caaacaagtc aaaaacgtgt ataataaaat aagttgtaca tagacacggt ctgttccggt
     1081 tgcccggaca ggcacagcac aagaaaggac aaaagaaaac acatgaaaaa attgattacc
     1141 gtactgcatg gtttctgtat ggcgctggcg gacagcgtgc cgggtgtttc cggcggcacg
     1201 atcgcatttc tgatgggctt ttatgacaag ttcatcggct ctctgaatgc gctgatctcc
     1261 ggcacaaagg cggagcgcat tgatgcgctc aagtatctcg taaagctggg catcggctgg
     1321 gcggtcggct tctgctcttc cgttctggtt ctggcgaatg tctttgagtc ccagatctac
     1381 cgggtctctt ccctgttcat cggctttatc atctttgcga ttccggtagt tgcctatgag
     1441 gaacgaaagg tattgaaaga gcgctggaaa atgtgctttt tcgttctgat cggtgcagcg
     1501 cttgtggcag cgatcaccta tgcaaacacc tttgtaggcg gcggcaagga cttgagtcat
     1561 cccggcatcg gaatgtatct gtacgctttc gtcgtcggcg ctgttgccat ctgcgctatg
     1621 gttctgcccg gtatttccgg ctctaccctg ctgctgatct tcggcttgta tattcctatc
     1681 atcggcggca ttcacgatct gctgcatctg gatttccata tgctgcccat tctgatcgta
     1741 tttggactgg gcgttatcac cggcatcatc agcatcatca agctgatcca gaacgcactg
     1801 accaaacacc gtggcgcaac ggtctatctg atcctgggca tgatgctcgg ctcgctctac
     1861 tccgttttca tgggtcccac aacgctggaa aaaccgcagg atgccatgag tctgcacacc
     1921 ttcagcatcc tgttcttcct catcggcggc gtggtgatcc tgggaatgca ggcgctgaaa
     1981 atgctctcgg aaaagaacag cgacaccaca gctgtaacaa aagagaattc gacaaaataa
     2041 accggatttc tacataggaa tgtgattttg tagggggcga tgcccacatc gccccgtatc
     2101 cccaaaaagg gaaacatttc gcatcgggcg gatgtgggca tccgccccta cataaaccta
     2161 acacaaaaag ggcttaccga aaaaacggta agcccttttt tagaaatgaa tgcaatatat
     2221 tttgcaaggg attcttccct tacgagatct tcttgatcca gcgcagaata tctgcctccg
     2281 gtgtcacaag gcggtcagag tcgaagtgaa tgttcagtcc gctctgcacc tttgcgccgg
     2341 aacacatctg cgcaatatcc tgaaacgtat gtcccagcca gccgccgttt gtggcaaacg
     2401 ggtagatcac ctttccggaa aaatcctggt gcgccaaaag tgtgcgcatt gccggggcaa
     2461 atgtatacca ccagaccggt gttcccagga taatggtgtc atactggctg aggtcggcgg
     2521 aaatcggttt cagttccggt tcaaaaccgc tgttgacctc acgctgtccc tggtctacga
     2581 ctgcgttata gctgccggta tacggagtga cggtctcgat ttcgcacaga tcggcgcctg
     2641 tctcctgctg aaccagcttt gcgatacggc gtgtgttgcc gccgtaggaa taaaacagta
     2701 ccagtgcctt catgcctcat agacctcctt tgccatgcgg aacgctgccc atgctttcgg
     2761 ccagcccaca tagaaagcga cgtgggtgat gatctctgcc atttctgacg ccgtgatgcc
     2821 gttctttttg gcggactcca ggtgatagcg gaacgagttg tctgtcagtc ccatggcgat
     2881 cagagagacc acggtgacca ggctgcggtc acgcagagag agttcttcct ctctggacca
     2941 cacctcacca aagaggacat cgtcgttgag ttctgcgaat ttcggtgcaa attctcccag
     3001 ggcatctctg cctgcggttt gtttcactgc cataaaatca aatcctttct cttttcttat
     3061 ggcaataccg cacaaagctt gtgcgtaaga tgcgtagctg gatttttcgg cagattgcat
     3121 agaaattccg caggcatact gtcgtatggc aagggatttc tgggcaatat gacggaaaat
     3181 cagcaagcag attgcgtgca aagccgccag gcgggctttg tgcggtattg ccttatctta
     3241 aagcgcattg tatgcttcgt cgctgaccgg ctcgcaccat tcattactgc agttttcgcc
     3301 ggggcattct actgcaatgt ggctgaacca gctgtccttc ttggcaccgt gccagtgctt
     3361 gacctccggc ggaatggtaa tgaccatgcc cggtttcagt tccacagccg gcttgccctc
     3421 ttcctgatac cagccctcgc cggcagtgca gaccaggatc tgtccgccgc ctttggtggc
     3481 gtggtgaatg tgccagttgt tccggcagcc cggttcaaag gtcacatttg ccagaaatac
     3541 cggtgctttt cccggttcgg tcagcggttt caaaaacgac tgtccgacaa aatactgtgc
     3601 atatgcgtca ttgggtgcgc cgatgccgaa cgcatctgcg gtttcaaact gtgctttctc
     3661 tgtatatttc atgagcgttt tctcctttta atcatgcact ttcagctggt gcagcatttt
     3721 cacaaaatcc gggttattgt gatccacgat ctcgctgtgt ccgatgtcca gctttgcgat
     3781 ctctgccata tcctcttccg acagggtgaa atcccagatg tcgaaattct gtgccatgcg
     3841 ctctgccttg acagacttcg gaatcaccac aacgccacgc tgtacattcc agcgcagtgc
     3901 cacctgtgcc gggcttttgc cgtacttttc gccgatggcg gtcagtaccg gatgggtgaa
     3961 aatgccgtga ttcccctctg cgaaaggtcc ccatgcctcc ggtgttacgc cgtattcctg
     4021 catcagtttc agcgcatccg gctgctggaa gaacgggtgg agttccacct ggttcactgc
     4081 cggaatgacc gaaacagtgg cgcacaggtc tgccaggcgt gccgggtaga agttgctgac
     4141 accgatggca cggagttttc cggcatggta tgcctcttcc atggcacgat atgcgcccac
     4201 atagtctccc atgggctggt ggatcagata cagatccaga taatccagtc tcagcttttt
     4261 cagcgaagta tcgatggctt tctttgcgcc ctcatagctt gcgtcctgca cccagagctt
     4321 tgtggtgatg aacagctctt cccgaggcac accgcttttg gcaattgccg cacccactgc
     4381 ctcttcgtta aagtatgcgg cagcggtatc gatcagccga tagcctgcgg cgatggcatc
     4441 ggaaactgcc tgctcacaaa ccgcagcgtc cggcacctgg aacacgccaa agccttccag
     4501 cggcattttt acgccattgt tcaacgttac aaattccatt tcggtttcct cctatgtcta
     4561 aatttatgcc tgctccggtt tcagcagccc gtagaaatac gatgctgttg cgccgccgtc
     4621 catcaaaaat gtggaaccgg taataaatgc gcccttccag ctcatcagca gttctgctac
     4681 atttgccact tcatctgccg ttcccggtct gcccgccgga cacttggcga acatattctg
     4741 ataaaatgcg ccacgctctc cgtggaattc gtctatggca agcggtgtga caataatgcc
     4801 cggcgcaatg gcgttgatcc ttgcgccacg ttcaccccaa tgcaccgcct ctgccatgac
     4861 acgcttttca ttgcagcgct ttgccagctg gtacgcatgg agcgtatccc ggattttttc
     4921 cggctggagc atctccaggg agagcagttc ctctgccggt gtttcggcga gcagtgcgtc
     4981 ctcttctgcg gtaagctggg gcatccggaa accggactga ctggaaatcg tcacgccgac
     5041 accgccctct gcaatatgtg tccgcatctc ttccagcagc attgccgtac cataaaggtc
     5101 tacacgcaga atggtttcca cggatgcctg gctgggtgaa acaccggctg cattgaccag
     5161 catctgaatg ctgccgtact gttccgcagc ggcaatggtc tgccggatgg attcccggga
     5221 agagagatcg gtctccatcg gaacggcgtc aaatccggca tcgcacatgg ttttggcgat
     5281 ttccccgggc attttccgca cgcttgtctc cgatgacgat tttcatgcca tagcccatcc
     5341 gccgggcgat cgccataccg atctgtcctg caccaatcaa tagtaatacg tttttcattg
     5401 gcttagtcct ttttgctcca cttttccagc gccggctttg cctggtctac gctgctgccg
     5461 taaatggaaa gtcccctggc aaccaatgcg ccctttgccg cacgctgaat gtccttttcc
     5521 gtaccgctca gtccgctgcc ctcatgggta cagaacggat ggatggtctt tccggaaaaa
     5581 tcgaaatgct ccaaaaatgt atacactgcc atgggcatgg ttccccagta gttgggatag
     5641 cccaggtaga tctcgtcgta ttcgtccagg ctgtccggca gcgtcaccag ctccggtctt
     5701 gcgccagcct gcaaatcttt cttcgcctgg gcaatgcagg tgtcataatc tgccgcatag
     5761 ggaaccttct gttcgatctg aaacagatct gccccggtca gttccgccag catcttcgcc
     5821 gccttttctg tgtttccctc tgtgatatag cggcgctgcc cgccgaagta gttttcgtct
     5881 gctctggaat aatatgcgat cagttttttc atggatcgtc tgctcctttc tgaattatct
     5941 gccgcaaagc cgcttttccg gctctgccct gcctggttat cttatctctt atctatagta
     6001 taccgcaaac cgccttgaca gggaatcccc gatgtgttat aatcatatta accaaaagtt
     6061 gatacgaggt gatagtcatg tataatcccc tgctgacggc gttcgtttgt gcggcagact
     6121 gcggcagttt taccaaagcg gcagaaaagc tgtacctctc gcccactgcc gtcatgaaac
     6181 agataaacac cctggaatcc catttgcagt tacagctgat ttcccgcacc aaccagggca
     6241 tccgactcac accggcagga gaatccatct atgcagacgc aaagtttctg tttgactatt
     6301 ccgcccgtgc cgtgcgccgt gccagacagc ggatgtcgga ctatgagaaa acattttgcg
     6361 tggggacttc tatcctgaac ccctgcaagc cttttgtaga cctgtggagc cgtctttcct
     6421 cccattttcc gggatatcgg ctcaacattg tgccgtttga ggacgaacac accaccattt
     6481 tacaggagat ctccgcactg ggcgaaaagt tcgatttcct ggtgggcgta tgcgattccg
     6541 caaaatggct gctgcactgc cactttctcc aactgggaac ttaccggaaa tgcgtagcgg
     6601 tgcgcaccgg gcatcctctg gcatccaaag aacggctcac tatctctgac ctctacgggc
     6661 agaatctgat gatggtaccc cgtggagact ctgccatcaa cgacaagatc catcatgacc
     6721 tggaagtctg tcatccgcag attcacatta tcgatacgcc gcaatattat gatatgagcg
     6781 tattcaatca ctgcgcccag accgatgacg tcttgctgac catcgagtgc tggcgtgacg
     6841 tgcatcccct gctggtgacc attccggtgg actgggattt tgccatccca tacggcatcc
     6901 tctatgacgt acatcccccg gaagatgtaa tggaactgat cgaactggta aaggcacaga
     6961 tgcaacagta agtgcctttg ccgacagact ggaggcagac aaatgaatct ggaaattttc
     7021 cgtggaatcc tgatcccgtt tctgggaacg acgctgggtg cggcgtgcgt cttttttatg
     7081 aagcgctcgc tctccagcag cattcagcgt gcgctgaccg gctttgccgc cggcattatg
     7141 acagccgcct ccgtatggag cctgctcatc ccggcgatgg aacaatgcgc ctcaatggga
     7201 aagctggcgt ttctgccggc ggcggtgggc tttctggtgg gtatcttttt cctgctgggg
     7261 ctggaccatc tcatcccgca cctgcacatg aacagcagtg aggcagaagg tccgaaaagc
     7321 aagctgaaaa agaccaccat gctggtgctg gcggtgactc tgcacaacat tccggagggc
     7381 atggcggtag gcgtggtcta cgccggctgg gcatccggga atatgtccat ctccgctgcc
     7441 gctgcgctgg cgctctcggt gggcatcgcc atccagaact tcccggaggg tgctattatt
     7501 tctatgcccc tgcgtgcaga gggcatggga aagggcaagg cgtttctgta cgggacgctc
     7561 tccggcgtgg tagagccgat cggtgcagta ctgactgtac tggcggctgg actggtactg
     7621 ccgatcctgc cctatctgct gagctttgcc gcaggcgcta tgatctacgt ggtggtggaa
     7681 gaactgatcc cggaaatgtc cgaggcgccc cattctaacg tgggaacgat tttctacgca
     7741 gtggggtttg tgctgatgat gacactggat gtggcgctgg gataagcagc acttgaagag
     7801 aacagccggc ttttgcagga gtgcagagcc ggctgtttgg ttttgcgtca ttgggattga
     7861 tctgactttt gtccgggcga acacgctttt tgacaaagcc ggaaaaatgt gctatactaa
     7921 gggtattcca aaaccgttct ctccggacgg aaattcaaaa agtcaggaac gtgatgcaaa
     7981 tgaagcgctt tcagcagaac atccgcaagg ttgtccccta tgtgccgggc gaccagccgg
     8041 attttcccga tatgataaaa ctgaacacca acgagaaccc ctatccgcct gcaccggcag
     8101 tgcaggaagt tctggcacag agccaggcag aacagatgcg gctctatccc gaccccacgg
     8161 cacaccagct gaacgctgct attgccaggg cttacggcgt agaggaatcc caggtgttca
     8221 ccggcgtcgg atcagacgat gtgttggcga tgatcttcca gacctttttc aacagcgatc
     8281 tgcccattct gttcccggac atcacctatg cgttttatcc ggtatggtgc gatctgttcc
     8341 gcatcccgta tgaagtgcag ccgctgaacg acaagctgga gatcgtgccg gaggactaca
     8401 acaagcccaa cggcggcatt gtgttcccga accccaacgc acccaccggc attgccctgc
     8461 cggcagccac tgtggaggag atcatccggc acaatccgga gtcactggtc gtggtagacg
     8521 aggcgtacgt ggacttcggc ggcgagtctg ttctgccgct gctgcaaaag tacgataatc
     8581 tgatcgtggt gcagactttc tccaagtccc gtgccatggc aggagagcgc atcggctttg
     8641 ccatcggctc tacggaactc atcaaggcgc tccacgacgt gaagtattcc ttcaactcct
     8701 acaccatgaa ccgctctgcc ctggcagccg gcacagcatc ggtacaggac agcgcctatt
     8761 tgcaggaaat cacggcaaag atcgtggcaa ccagagaaca caccaagcag gtgctggcgg
     8821 aaatgggctt tcagttcccg gattccaaga gcaacttcct gtttgtgacg cacccagact
     8881 gcgatgcaca cgagctgttt gaggcactgc gccaggcaca catcttcgtg cgctggttcg
     8941 actccccacg catccggaac tatctgcgca tcaccatcgg aacagatgca cagatggaat
     9001 cccttttcgc attcctccgg aagtatctga acaagtaatt atagtataag agcctgttcc
     9061 atatctttta tatacggctt ttcgagcatg attttgtcgt tggcaaggaa aaaagccata
     9121 ggcatactgt cgtatggcaa ggctttttga cgcagtcaac ggcaaaatct gccgaaaaaa
     9181 cgtgcggaaa aagatgtgga acaggctcta aaataacccc gtctcatcct ggttctttgt
     9241 gcgaaccggg gtgggacggg gttttgttta tttacggaca catcacacgc cgctgtgctt
     9301 tccacatatt ttctactttt cctccagata ctgctgcaat ccggaaaaaa tcgtaaacgc
     9361 cactttctgc tgatactcct ccgattccag cagcgccgcc tcgtctggat tggacagaaa
     9421 gccgcattcc accatgactg tgggattttc gctgaaatag cagagataca gctccttgcc
     9481 gcactgcttg atctccctgg cattgtccgg ctggagctgg gtcacaaagg cattttgcag
     9541 gcactgcgcc agtgtgccgc tgcgctttac gttgtccgaa tagaacatct gtgcgccgga
     9601 atacgccggg tcggtgaact gattctgatg caccgaaatg cacaccgcat tgtcatactg
     9661 gttgaacagc gccaggcggt tatccatgtc cgaactcttc tggttggcga tgccctctac
     9721 accgtcatcg tggatgctct tgtcagaatc ccgtgtgacc tccacggtgt agccctccat
     9781 gcgcagcaga tcacgcagac tcagcagaat attcagattg atgccctttt ccggcacgcc
     9841 gtttaccgag acacagccgc cgtctgcacc gccgtgtccg gcatccagta caattaccgg
     9901 cggctctttc tccggggctg tcatggtcgt gacggctcgc tgtatcctgg tgccgccgaa
     9961 atagaccgcc gccacactga cggcgctgac tgtcagaacg attcctgccc gtttccatat
    10021 ggttttccaa gtgcattgca taccgtgtcc tcctgcaaaa gcagcactgc accatcactg
    10081 cggcaggact gctggttttg ttttggtaca ctttatgcac aggaactgcg gagtatgccc
    10141 tatgcctgtt ccgcccggtg cagccgccgc tgaaaccacg ccagcagttc tgttttggac
    10201 atggctttct ggtgcgccgg ctgtgccagc tggaattcca gcggttccag ttcccgaatc
    10261 agctgccgca gctttgccgg acgctcatgc caggtgtcca ccaccagttc ctggtacacc
    10321 gcatcgccca gaaacaccat ttgctcttcc tgcacccaga cgatcaccgt atctctggcg
    10381 tgagcgctgg tcacgtgctg taacacacag gtgcagtttc ccaagtcaag ggtcatctgc
    10441 tctgaaaaga ttaccgtggg cagcgctacc cggatttgtt ccggttcggg gaaatgcgcc
    10501 tggatacgtg gcacgcacag ctgcggcacg atgcctttcc ggacgttttc cgccagggca
    10561 tccggtgtcc agcgcagccg ggagaccatc tccagagact gtctcgtctg cacacaggca
    10621 atggacggga tcgacagggc agacagcccg aaacagtgat cccagtggga atgggtcagc
    10681 accgtaaaat ccggctgggg aaatccggcg ttttccactg ccttttgata atcccggacg
    10741 tgttccggcg agttgccggc atccaccatc agcgaaaacc ggtcgccccg gatatacccc
    10801 agcgccgggc ggtcgttttc ctgcgaaaag ggcagctgat acactctggg ggtcagttgt
    10861 tccagttcca tcggcaatac ctcctgtttg gtctcttttc cgtgtttgct ttttcgttta
    10921 tccgtgtccc agcaccagca gctggaacag tgccatccac tcacgcaccc aataggtcgg
    10981 cacgtacagc ggattggtcc atgcgctttc gctctccgat tccagcccgg cacgtttcgc
    11041 caaaagcgac gcccgatact ggtgaaagcc gtctgttatg atgacaatgc gattgtccag
    11101 tccctgctcc tgcaaaatgg cggcggcgtg ttccagattc tcctgggtgt cctttgaggt
    11161 agtctctgcc gtgatgcggc tttcggcaat gccgtgttcc accagccagg ttttcatcgc
    11221 ctgtccctcc gggatatctt cccctgcgcc ctgtccgccg gtggtgatgc aggagacctc
    11281 cggattttcc tccagatact tctgcgccgc ctccagccgc cggagcagca tccggctggg
    11341 acgagtgcca cgcaccttac agcccagcac gaccacggtt tccggctgtg caggatggcg
    11401 caggacagcc cgtgtcatct gcacagacat caccgcagaa aacaccagac cagccgccgt
    11461 cagcacgcac accgcagaca gtccgatctt tccgcccagg tgtccccaca ggtgacaaat
    11521 ccaccgggca aacacctgcg gaaacagtgt cacaaacagc agaaacaggc acaccaggat
    11581 cccggcaagg ttccccggat tgcagatgcg tttgaaaatg ggaaatacaa aaaagaagag
    11641 cagtaccagc tccggcagta ggaatagata acgcagtttc atgtcagtag ttcctttctg
    11701 atattttgtg cagtatggtc atctgatatt ctatagtata gcatatctgc cgggatgtgt
    11761 caatttttta gatttctcgg aaatttttat cggctttttg tttgacatct gttagggaaa
    11821 cactgattaa atcttcccat tacgcagcaa atatggtata atagaa
//
LOCUS       DAWENO010000052        11770 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2312_length_11770_cov_12.467606, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000052 DAWENO010000000
VERSION     DAWENO010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_11770_cov_12.467606"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3176 a         2991 c         3196 g         2407 t
ORIGIN      
        1 caagctttgt gcggtattgc ctaaaatttt attgtgttaa ggtaagcaaa agccgcacat
       61 gacttcactc atgtgcggct ttttgaaaag ggaaaccggg cagtcatttt tgcaggaaaa
      121 gcggagacgg tttctgtttg ccgtacatag aacggtttcc gctgcacagc cgggaaatgg
      181 tgcagacaac ggcaaaatag ggcaggcagg agaatccgaa cacttccgtt ccgatgcaga
      241 tgggcgccag ccaggtgttg gaggcactcc cgaacactgc cgcatagcag agcgccgcac
      301 caaacacagc cgggagtccg aacagcggcg cagcgacagc gcccaggcag gcaccgatgg
      361 cgaacagcgg cgtgacttca ccgccctgga atccgacggc aagggtgcag acggtgaaca
      421 gcagcttgcc cagaaagtcg aaccagtaga tcgtttcgcc gttgcaggca agggaaatca
      481 aatttgtgcc gagtccggta tagcgtccct ttccgcagag gaagagaagc agcgccagca
      541 ctgcaccgca gacggcgatg cgcagcaggg gattggggaa cagttttgcc gcccaggatt
      601 ttccctgttt cagcagcagg gtgaagatac cgccggcaat gccggagaaa acgcccagca
      661 gaaggatcgg cacggaaaac ggtgcagtat ccacatccgg cagagcaacc tgaaaggtct
      721 ccagccccag cgccgcagag acccagttgg cagaaacggc ggcgagaacg gcagggagca
      781 gcgcacggta ttccagcctg cccactgcca ggacttccag ggcaaagcag actgccgcca
      841 gcggcgcatg gaacagaccg ctgaaaccgg cagccatgcc ggcaatggtc agaatgggga
      901 cggcatcggg gatatcccgg aaccagggga tgcgcctgcc catgttgtgg gagaccgcag
      961 cacccagctg aaccgcaacg ccttcccgtc cggcacttcc gccgcagaga tgggtgagcc
     1021 aggtgctgcc gatcagaagc ggaatgagcc ggagcggaat gcgttttgcc ttgccgtcgg
     1081 cggcgtcgaa gatgaggctc atgccctggg cgctctcttt gccgtatttc tggtagaccc
     1141 agaccatcag aacgccggca atcggcagaa acggcaggca ccagagcagg tggttgctgc
     1201 ggaatgtgcc gatccacagg agaatgcgcc cgaatccggc ggtgagcgca ccggtcacaa
     1261 tgccggcggc gatcgccaga ccgcagagcg ctgcggtggg aagtatggaa ttgcgaatgt
     1321 tgcttttcat agacaggacc tccggaaaaa taaaaaagcc gacagcatct gcgtcaaatg
     1381 cagatgtcat cagcttgtca gcggttttgg aattccaggg gagaacatca ttccccgaat
     1441 cagattgtac ttatattata cagtatcttt cagaatctgt caagaaaaaa tcagagagga
     1501 atcattgcca tgaaagaaca gcgaaaaatc gtccttggcg gcgagacggt ctcctatacc
     1561 ctggaacgca aggcagtgaa aaatatcaat ctgcgtctac gaccggatac cgggctgtcc
     1621 gtttctgcgc cgaagtatgt ggcgctgtcc caggtggagc gtgtgctgat gcagcaccag
     1681 cagaagatcc tggagacact gcgccagtac cagaccgcag cggcggaaag gcggcagcag
     1741 tatccccgga gcttttccac cggagaaacg gtactgtacc tggggaaata ctgtactctg
     1801 gaagttctcc ggggcagccg ggaatgcgtg caggtgcagg gggacaagct gcttttgctg
     1861 gtgaaacatc cggaggatgc ccagtgccgg gagcgtgtgt tcaacagctg gtgggagacc
     1921 agctgcgaaa aggcggtgcg caatctctgc cgggcggtct atccggtgtt tgaggcgaaa
     1981 ggcgtggcgt tcccggagat ccggtttcgc aggatggtcg ctcagtgggg aaactgccgc
     2041 ccgactcgtg gcgtgctgac attcaatctg cggctgctgg ctgcaccggt gcgctgcatc
     2101 gaatatgtgg tgatccatga gtttacccac tttctccatc ccgaccactc accggcgttt
     2161 tatgcggaga tcgccgcgga actgccggac tggaaacagc tgcaggaaac tttgcagcat
     2221 atggtagaaa cacggatata gcgcgggatt tccggcaaaa ttccgaaaac cagccgtttt
     2281 tttccgatcg tttctccgga gagttgacaa tatggctaaa aaagccgcag ccgctttgtt
     2341 cagtttccat atatttttcg tgtgaaagga aaaaactgtt gatttttttt gtgcggtatg
     2401 ataaaataaa cttcgggcat gtctgcactt tttttgagaa ttgtcctaga gtggggcaga
     2461 atgtgcagaa gaaatgcgaa aaacgaaagg agcttttttt atgaagaaac ttgggctcgg
     2521 taaacgggtg ctggcgtgcg ctgccagtgc tgcgacgtta ctgaccggaa ccacagcgtt
     2581 gtctggactg acaacactgg gaagcatggc ggcatctgct gcatcttatg acaactatgc
     2641 aaagttgctg cagtactcca tgtatttcta cgacggcaat atgtgcggtt ctgacgttga
     2701 cactgcctct cagttcagct ggagaggcag ctgtcacggc agcgatgaag tagacggcgg
     2761 tttccacgat gcaggcgacc acgtaaagtt tggtctgccg gctggctaca ccgcttctac
     2821 tctgggctgg ggctattatg aattcaagga cagctatgat gcactgggac agacggctca
     2881 tttgcaggcg ctgaccaacc gtttctgcga tttcttcaag gcaagcacaa agctgagcgg
     2941 cgacaccgta acaagctttt gctaccaggt aggcgtagga caggctgacc acgacgtctg
     3001 gtgttctccg gaatcccaga acgatcagag cctgcgtacc gcatactgga cttctgatga
     3061 tgccagcgac atcgccgcag aatatgcagc agcactggca gtgaactaca tcaacttcgg
     3121 caatgcagaa gacctgaagt atgcaaaggc actgtacaat tattccatca agaccaacaa
     3181 ggcaaattgt ccggaggctg caaacttcta caactcctat gactattatg atgaccaggc
     3241 atgggctgca ggctggctgt acctggcaac caacgacagc acctacagct cgttcctgaa
     3301 taagttcatg aacgacacca atgcaggcaa gtccggtcag tccggatgca agtggggcgt
     3361 atactccacc atgtgctgga ataatgtcag cctgggcgct gcaatcctcc agagtgagat
     3421 caccggcgat gcaatggact gggcaaaggt cactacatat ctgaacggca aatgcaccag
     3481 tgaaagcacc tattactgcg agagcgactg gggcagcgca cgttataaca ctgcactgca
     3541 gatggcagca ctggctacca cgaagtattc tgcaaagagc ggcatggatt attccagctg
     3601 gtgcaaggca cagatgggca tgatcctggg caacaacccg aagaatgtga atttcgttgt
     3661 cggcatggac agcaattctg caaagtatcc gcaccacaga gctgcatccg gctatcagag
     3721 ctttgacgaa atgaagggca agaccgacta cagcagcaat ggccacacgc tggtcggcgc
     3781 tctggtcggc ggtccggcag attccaactt cacttatacc gattctgtaa acgactatca
     3841 ggcaaacgaa gttgccctgg actataacgc aggtctggtc ggtgcagcag caggtctgta
     3901 ctccatttac aagactggtt ctgtagattc tagcattgag ggtgtcagcg gcggcgctgt
     3961 agttaccact gctactacaa agggaactac cacaaccacc accggaaaga ccactgcaac
     4021 cactaaaaaa accacaacca ctactactac aaaggctact cagaagacaa ctgtgacaac
     4081 tactactgta ccgggcggca gcggcaagac tgcaaacatc agcaccggta cacagactgg
     4141 taccgcaggc gacaagcagg catatgcaga attcgcaccg aacggcgcaa agactgcgac
     4201 tctgtactat aaggtcaaga ccagcgacac caactccagc ggtgcattcg gcacatggac
     4261 cggtacctgg gaacaggaag actttgaagt aactgtcggc gcaaacggcg agtgcaaggc
     4321 aacctataac attccgtcca acgtaggaca gaccgttaag gccatggtct tctatccgga
     4381 tgcaagcagc gtagagatcg aaaaggttgt tctggatgag agcagcacac cggtcaccac
     4441 aacagttacc tcaaacggca acacctccgg cggcaagaac gcaaacatca ccaccggcac
     4501 acagactggt actgcaggcg acaagcaact gtatgcagaa tttgcaccga acggcgcaaa
     4561 gactgcaact ctgtattata ccatttccac caaggatacc aactccagcg gtgcattcgg
     4621 cacatggacc ggtacctggg aacaggaaga ctttgaagta actgtcggcg caaacggaca
     4681 gtgcaaggta acctataaca tcccgtccaa cgttggacag accgtaaagg caatggtatt
     4741 ctatccggac gcaagcagcg tcaagatcga caaggtcgtt ctggacgagg gcagcactcc
     4801 ggcaaccact gcatccagca caaagcagaa cccgacaact actgctacta ctgtagcacc
     4861 gggcggagat gacaagactg caaacatcag caccggcaca cagaccggta ctgcaggcga
     4921 caagcagctg tatgcagaat ttgctccgaa cggcgcaaag tccgcaaccc tgtactacac
     4981 catctccacc aaggatacca actccagcgg cgcattcggt acatggaccg gtacctggga
     5041 acagaccgac tttgatgtta ccgttggcgc aaacggtcag tgcaccgcag attatgtgat
     5101 cccgtccaat gtcggacaga ccgtaaaggc aatggtattc tatccggatg caagcagcgt
     5161 caagatcgac aaggtcgttc ttcactacgg caacaccgta actaccggca caacaaaggc
     5221 acagccctcc agcggcggca agtacaccaa ggatgtaaac cagtctgtcg tttatgacga
     5281 actgcctgct ggtgataaga tgctgggctg gaagtggtcc gacctgggtg ttccggctgg
     5341 cgaaaaggta accaaggttg agatcaacat ctccaccagc aagaagcaga tcggcaagtg
     5401 gcagggcgca ttcggctctt ccaccagcgt agcaccggat tactggacac agtctgatga
     5461 tatggaacag accatcgacg gcaagaccgg cagcattgta tggaacgtag attctgcaac
     5521 ctctgagatc atccagaccc agtacggcgg cgagctgaag ttcggcgttt ggtggatcga
     5581 ctgcaacaag ttcaccatcg actccatcac agtttacaca gacgcttacg gcggcagcag
     5641 cacaacagtt accacttcca agacaagtgc aactacacag aagacaaccg caactacttc
     5701 caagacaact gcgaagacaa cttctaccac aaaatccagc acacagaagc ccagcgatct
     5761 gccgtctgca agcatgtacg gcgacgtaaa cctggacggt cgtgtagaca tcacagacgc
     5821 tgttatcctg aacaaggcag ttgcaggcgc aatctccctg aatccgcagc agcaggcaaa
     5881 tgcagactgc tgcaccgatg acggcgtagg cgctaacgat gcgatcgttc tgctgaagtt
     5941 cctggtttct ctggtaaagt ctctgccgtc tgcagactaa gccacaggcg ctgaaacggc
     6001 gtaaaaaatt atcgaaatcg tatattcaaa agcggatatc gtcaaaactt ttggcggtat
     6061 ccgcttttct gtgacagcat atccgaaaaa actgtcgtta catatgtcat gttgcacaaa
     6121 aagagaggaa actgtttagc aagttttact gaaatttgaa aatccgcttt acaacaaaat
     6181 tctcatgtgt tataataaat gaaacgagaa agatatgccc tgcgacggga tacgtgcgtc
     6241 ttgaaatccg gtccctgcgg tgtgtatcag ccggcgagtt cggcagaaga aatcgttgca
     6301 aaaggaatat gcgtacctat attccaaaat ctcaaatcat tcattttcaa ggaggatatc
     6361 tatgctgaag aaaaaactcg gcaagaaggc acttgccttt gcttccgctg cagtgatggc
     6421 agtttctgct accgctacag gtctgacagc gctgtctatg actgcgttcg caggcgagga
     6481 gctgggcgaa ggtactttcg aaaacggaaa gggcttgcct tggcacattt gtgaaaatgc
     6541 aactgctgtc atgaagttcg atatcaccga cggtatctat gcgatcaagc tggaaaatgt
     6601 cggcggtact tccaacggcg gtgagagccg ctgggactgc cagttccgtc acagaaacct
     6661 gaccattgaa agcggtcaca cctatcgtat cacctacagc gtaaagccgt ctaacagcgg
     6721 tcacatgtac gcaaagctgg gtaatatgtc caacgacgac caggaactgt ggcacagcaa
     6781 cggtgaagag ctgagcatga gctacgaaga aggtctgaca cagacacagc tggaagacaa
     6841 gctgaagagc gcttccaaga ccggcaacaa ggtagactac ggtcagggct gggatgcttg
     6901 gtatcacaag gaatatccgg caaaccagtg gaccactgtt gcttatgaat tcactgctga
     6961 ccagaccgtt gagggtactg cagagtggac cttccacatg ggcggtcagg gtgcatatgc
     7021 aacacagggc gactgcttcc cgaaggacac cgttattctg ttcgataacc tggcactcat
     7081 cgatatgacc gatgacaaga cagactataa ggcagaggca gcttatgagc cgaccggcgt
     7141 tgaagtaaac caggttggtt actatccgaa cggtaagaag gtcgctactg ttgttctgtc
     7201 cgacggcgac acacagaagt atgactatga gatcaaggat gccagcggca agaccgttta
     7261 cagcggtact actgacggca acacccagta tgacaagtct ggcgcatggg attacaccca
     7321 gcagatcgac ttcaccgatt tcaccacaga gggcaagggc tacacactga ctgttgcagg
     7381 caagacctct ctgccgtttg atatcgacaa gaacctgtat gagaagtaca acgaaaagag
     7441 catgctgact tatgccctga actacttcta tcagaaccgt gcaatggata ctgacgacca
     7501 gtacattccg tctccgcaga ctgtagacgg tagcagcaaa actctgggtc gtaaggattc
     7561 caaccactgg ccgaacgata ctgcttacat cgcagacaag tgggtttaca tctacacttc
     7621 caagccgagc tacagccagt ccatcgacgt atccggcggt tggttcgacg caggtgacta
     7681 tggtaagtac gttgtaaacg gcggtatctc tctgtggact ctgatgaaca tgtatgagag
     7741 atccaagatg gtaggcaagg ctgacaagtt cggcgacgac tcctccgtta tgaccattcc
     7801 ggaaaacaaa aacggcattc cggatatcct agatgagtgc aagatcgaac tggacttctt
     7861 cctcaagatg atacgtgacg acggtatggt ttaccacaag gctcacgact ataagtggac
     7921 cggtctggct gtcgctccgt atgaccagaa cgaaaacggc aaggaaaaca aggctccgat
     7981 gcgaatcgtt aagccggtta cctatgcagc aaccctgaac gcatctgcag catttgcaca
     8041 ggcagctcgt ctgttcaagg actatgatgc agcttatgca aagaccatgg aagacgcagc
     8101 aatcaagacc tatgctgcag cacagaagaa ctacaagccg ttcacaagct ggggcggcga
     8161 caccaagggt gaaggcggca tctccgctga catcatgtac gctccgctgg atcagaacaa
     8221 gggcggtggc ccgtacggtg ataccgaggt cagcgacgaa ttctactggg cagcttgcga
     8281 actgtacatc acaaccggcg acaagaccta ttatgatgaa ctgatgaagt atggtaccaa
     8341 cgcttatggt actgacaatg caaaggcact ggaaatctcc acaactctgg ttggcggtga
     8401 gaacaacggt tcattctccc tgttcacatg gggtactctg aactccgttg gttctatctc
     8461 cctgtatgta aactctcagg atatgctgga taagggtctg ctgactcagg atgaagtaaa
     8521 caccctgaag gcacaggttc tgaaggcagc tgactccgtt ctggaagtac agaacaagtc
     8581 tgcatacggc atcccgtacg taggtcatga ctatgacaca actgtatgga agtatgacgc
     8641 agcatccggc aagggtgaat cccagactct gagcctggaa ggcggttatg aatggggttc
     8701 caactccatg gtaatcaaca actctatggc actggcactg gcttatgacg catctaagga
     8761 tgtaaagtac atcgatggtg ttactaccgc aatggactac ctgatgggcc gcaacccgct
     8821 ggaacagggc tatgtaaccg gttacggtga acaccccaca aagtacccgc accacagatt
     8881 ttggtctggt cagctgaaca gcaacgactt cccgtatgct ccgtacggcg ttctgtccgg
     8941 cggcccgaac tccaacatgg aagacccgat ggttcagggt cagggctaca aggtaggttc
     9001 tatcgctccg atgaagtgct acctggataa cgttgaggct tggtctgtaa acgagtgcac
     9061 catcaactgg aactctccgc tgtgctgggt tgcatccttc ctggatgacg aagctccgaa
     9121 catcgttcgt gactccagcg atacaaagcc gaccacaaca actgacggca agactacaac
     9181 aactgagact acagcaacca ctgctacaag cgacaacgat tcttcctcta ctgcatctac
     9241 agataagagc ggcgaatcca ctactaccac cacaaacggc ggttctgtaa ctcctggcga
     9301 cgttctgctt ggcgatacca acctggacgg ccgtgtagac atcaccgacg ctgttctgct
     9361 gaacaagaag gctgcaaacg cagttgactt caacgcacag cagctgctga acggtgactg
     9421 ctacgatcag aatggcgaaa tcgacggcaa tgatgctacc gcactgctga agttcctggt
     9481 acacatcatc aaggctctgc cggagacttc cgatctgaac gcataatcga agtcgttttt
     9541 tgaaacgatc tccgtaaata aaaaaggcgc tgtggctgta atggctgcag cgcctttttg
     9601 tatttgcaga aaatgtcgaa atttcctatt gacaatcccg gcggaatcat gtataataaa
     9661 aacaacagta gtgcggcata tttcgtgtgg cactgccggc ataccttata ccttatcaag
     9721 agtggtggag gaacaggtcc tgtgaagccc ggcaaccggt tgacgttttc gtcacatacg
     9781 gtgccaattc ctgcggtata accgagagat gaggggtttg tacaaaaagt tttggacacc
     9841 ctgcgagggt gtcttttttt gatactattc ccgcgagaca gaaaggaaca aatcgaatgg
     9901 caagaactct ttttacatcc gaatccgtaa cagaaggaca tccggacaag atctgcgacc
     9961 agatctctga cgcagtgctg gacgctatcc tggaacagga cagcaaggca cgtgttgcct
    10021 gcgaaactac agtcgctacc ggcatggtgc tgtgcatggg tgagatctcc accacggcca
    10081 aggtggacat ccagcagatc gcacgggacg tgatcgtgga catcggctat gacagagcaa
    10141 agtacggctt tgacggctat acctgttctg tgctccaggc gatcaacaag cagtccccgg
    10201 atattgcaat gggtgtaaac gatgcgatcg aaaccagagg cggcgagact accgagaccg
    10261 gcgccggcga ccagggcatg atgtttggtt atgcctgcga tgagacgccg gaactgatgc
    10321 cgctggcaat ttcgctgtcc cataagctgt gcaaaaagct ggcagaactg cgaaaggata
    10381 agacactgac ttatctccgc ccggacggca aggcacaggt caccgtggaa tacgatgggg
    10441 acaagccgat tcgtgtggat acagttgtca tctccaccca gcacgctgca gatgtgactc
    10501 tggagaccat tcgccgtgac atgatgcact atgtgatccc ggctgtgatc ccggcagaac
    10561 tgctggacga aaacactaag tatttcatca accccaccgg acgtttcgtg gtaggcggtc
    10621 ctcagggcga cagcggtcta accggacgga agatcatcgt agacacctac ggcggcacag
    10681 ctcgtcacgg cggcggcgca ttcagcggca aggatccgac gaaggttgac cgttccgctg
    10741 cctatgcgac tcgttatatt gcgaagaata tcgttgcggc tggtctggca aagcgctgcg
    10801 aggtgcagct ggcatatgcc atcggtgtgg caaatccggt ttccattcgt gtggatacat
    10861 tcggcaccgg cactgtttct gaggaagtgc tggcaaaggc agtaccggaa gtgttccaac
    10921 tgacaccgga cggtatcatt gagatgctgg atctgcgcaa gccgcagtac cggaaactgg
    10981 ctgcctatgg ccacatgggt cgtgaagacc tgggcgtggc atgggagcgc cgggacaagg
    11041 tagatgctct gaaagcagca gttgcaaagg tgcagtaagc ggcatctgta tcagttgaat
    11101 tgataggaac aaggcttttc ccgagggatt ggggaaagcc ttttctgtta ctttcaaaaa
    11161 agctgtgacc aattcggcaa aaaagcggtc tataagagtg aaaggaagtg aaaatgatgc
    11221 aggatgaaga gttgattcgt ctgtatgtaa cacgttctga acgtgcaatt gcgctgacca
    11281 gcgataaata cggcggctat tgccatacga tcgcctatca gattttgaaa gatttagagg
    11341 attcggaaga atgcgtaaat gatacttact ggaaagcgtg gaatgtgatc ccgccaacga
    11401 tgccgaaatg tttcaaagcc tttcttggga aaattacccg gaatctggca ctgcaccgct
    11461 gggagaaaaa cagcacgaaa aaacgtggcg gaaatcagat tccgttcctg ctggatgaat
    11521 tggcggaatg tatcccatcc caggaagaca ttccgaaaaa ggtagaggat gctgaaatat
    11581 gcgcactgtg caatcagttt ttagagatgc tttccaagga aaaacgcatg gtatttgtgc
    11641 ggcggtattg gtattgtgat tcggtatcgg atatcgcaaa aatggtatca ttttggagaa
    11701 agcaaagtga agatgatgct gtcccggaca cgaaaagaac ttcgggatta tctggaaaag
    11761 gaaggtgttg
//
LOCUS       DAWENO010000053        11711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2318_length_11711_cov_10.148164, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000053 DAWENO010000000
VERSION     DAWENO010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2318_length_11711_cov_10.148164"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3093 a         2533 c         3069 g         3016 t
ORIGIN      
        1 gtgcatatca ataatgatac aggcgaggtt tttgcagttc agaattttga taagatttca
       61 attaaaatag caaattcgat tgaagaactt cttggagaaa tgaaaggcgt atgggatatg
      121 tgagtaatgt gtaactatca atgtaaatat aattcctgct gcacgccgat ttcgccttgc
      181 agcgtataag tgactgtata ttcaacgccg ctctcagttt cgagggcggt tgtttttttg
      241 tccaggattt ctacctggga caaaaagttg tcttcatatc gctgtatctg ttcttctgca
      301 ttttgtgctg cctcggctgg cgttaaaata aaagtacggg tgcggtattc gtgaaatgtt
      361 tcctggtata tgccgatggg aagttctttc ccgaacagag aaaaccaatg ataaccggaa
      421 gtgcggatgt agtcatcaaa tggtggttct gtacttccca acgggatgtg ccaggtgaac
      481 aaatccaggt actggtgcgc cgttgtttcg ccggtaggtc tgcgcagtgt ctgctcatat
      541 gtacaggtga atttttctgt ttgtttgtac tgcccgatga tgctgcccat agcgtgtttg
      601 attccagtgt gttcggtttc atctgtgtaa agaccgctga ccagcaagtc ccctttttgt
      661 accggatcgc ctacgatttt catgagctgt ccacgataaa tcgaaacaga aacaatttga
      721 ccatcctggg atgcgatgat gttcgagggt tttcgtgtgt tctgcatttc tggttctggc
      781 gtgcgttcca tcacttctac aaccagacga tttccggtgt ggcgcattcc gacccatgca
      841 agttcgtcca tcgtggaacg aagggtatgt tcacagtgcg caaagtcgat tccaggaatc
      901 caactccccc gctgcacacc gcagtcctcc aggacggctt tgatttccgg aatggatgct
      961 gtttcttctc cggttacatc aatttccatg acgatattgg aacaataaaa gatcaaggcg
     1021 gctgaaaaaa gaatacccaa tgggatgcca aaacgaaaac gatattgatg caaaaactgc
     1081 tggagtgttt ttcggggcgt taatgtcagc tgaacatgat atgtttgcgc caactgttct
     1141 actgcttttc gatgcttggc atagaaccga aattgataga tattttcctc acagcgctgt
     1201 tttttgcagc atatgccggc agcgtgcatg gcgtttacaa atgcgtacag tctgccgccg
     1261 gcagcagcag cgtgatattc actccgcaga tgctgttcca tggcgcaccc cctttggtgt
     1321 aagtgtgata gattggattt gtccatgaat cactaggctt tccggagaac ggctgtcagc
     1381 ctgcaaatca cttccccaga tttccaatac cagttcggcg gtttgtacaa caattcgaat
     1441 atcgttgtat tccaggatgc ggcggcagtt ttccagatag atctctcggt ttccggtcag
     1501 ctgcaaatat gtttcgaact gcagcactcg ccggcctgca tctatgagcc gttttcccat
     1561 gacgatcacc tcgcaaaagc atatgcggag aaaaagcata ttatgtgctg tcagcgtcat
     1621 agcatggaat cgggaggtgc tgtttatgcc ccatgttttt cagaaacaat atttttggat
     1681 acctgtactt gtctgtgcaa tatgcttttg cgtatgtttt gcagaacaaa ttcagatgga
     1741 aatgcggtca gctttgacac gttgtttgac aacgatcatt ccctctctgt atgccatgat
     1801 gattctttcg cagatgcttt tggaaagcgg cgtatggcgc cttctggcga aaccgtttcg
     1861 atggttcgga gaaaaatgcc ttgggattgc agagccgtac tttgccgttt ttcttctcag
     1921 tcagttcgcc ggatatccgg ttggcgccgg aatgctttgc acgctagtaa aacagcactg
     1981 cataactaaa aaggatggcg aaaagctgtt atgtgtatgc tacggcggcg gacctgcttt
     2041 tttgttggga ttgttgggaa atgtcccctg ccgctccgca atcttttggc tgatattttc
     2101 tgcaaatatt ctatcgaata cagtcctctg taccttactg tttcgttttc atcctctgaa
     2161 aaatgatgta tcagaaagaa aggctataac gccggtttca gcagaaatgc tggtggactg
     2221 cacttcgggt gcaggaaagc tgctcttgaa gttgtgcggt gtgatactct gcttttctgc
     2281 atggattggg atggcggacg cagccggatt attccgtatt tgtcgtgagt ggacaatgaa
     2341 gattgaactt cccttttctg gctctgattt gctgcgatgt gttttagaaa tttccaattg
     2401 tgctgcgtta tcaatgccaa tgcgctggag agttccagtt ttggcggcgc tgctttcctt
     2461 tggcggtatt tgtgtcattt tacagattcg ggctgtggtt ggaacaagtt tatccatgaa
     2521 gctgtttatc acggtcagat tgctgtgcgg attgctttcc ggcggaattt gtgcggttgg
     2581 actgtacttt ctgccaattc caataacaga gacggtggtt tctgtttctg ccgttccgca
     2641 ggtatccggg aattcaatcg ttccgtctgt tttgctttta tgtatgatgc tgtttgtttt
     2701 tcgggaggaa tcttttcttc ataatcacaa aaaaattcgt gaaattgctt gactttttgg
     2761 atagaacata ctataatata agagtataaa tatgcttttt ttgaaaagca tatttatagc
     2821 tgctgtcacg gcgcaaagga aacagcagct gcattcattc agacattatt ttggaaaggg
     2881 gataatttat ccatgacaat acgtaaaaaa cttgctgcac ttgctgtttc cggcatgatg
     2941 atggcaactt gtctctctgc cagcattccg gcgattcaga cgagtctgac agctgccgca
     3001 gcggatgata acaacgatga ctggctgcac gcagagggca gcaaattgta tgattctcag
     3061 ggaaatcaag tgtggctgac cggtgcaaac tggtttggta tgaactgtac agagaatttc
     3121 ccgcatggtc tttggagtgc agatgtggat gaactgctct cttctgttgc agatcacggc
     3181 atcaatatca ttcgttttcc ggtatcgaca gaactgttgc tttcctggaa aaacggcaat
     3241 ccgctgacgc cggtaggact gaacgccgca aacggaaagg actactcctt taacccagat
     3301 ttttgcgatg caaacggaaa tacaatggac agcgaaggta tcttcgatgt gattctgaag
     3361 aagatgaaga agtacggcat caaggcactg atcgacgttc acagcccggc atcccacaat
     3421 tccggacaca actataattt gtggttttat caggatggcg cagctgatgc agataatatg
     3481 gctgttggat tttacagcaa agaaaaaatt acttacgatg attggattga gtccactgca
     3541 tggctggcag aaaagtacaa gaatgatgat acagtaattg cctacgattt gaaaaacgaa
     3601 ccgcatggca aacgtggata cagtggttcc agctgcccga cagatatggc aaaatgggac
     3661 gattccaccg atcagaacaa ctgggcatac gccgctacag aatgcggcaa tgcaattctg
     3721 gacaagaacc ccaatgcttt gattttgatt gaaggcgtag agcagtatcc gaaaacggat
     3781 aagggttaca cctatgatac agcagatatc tggcaggctc cggcagatca gtcaccttgg
     3841 tacggcgcat ggtggggtgg caatctacgt ggtgtaaagg attatccgat cgacttcggt
     3901 tccgcagacc gcaacagcca gattgtgtat tctccgcatg actacggtcc gtctgtatac
     3961 aaccagacgt ggtttgacaa ggactttacc acccagacac tgctggatga ttactggtat
     4021 gacacgtggg catacatcaa cgatcaagac atcgctcccc tgctgatcgg cgaatggggc
     4081 ggtcacatgg acggcggcaa gaaccagaag tggatgaccc tgttgcgtga ctatatgatt
     4141 gacaatcaca tcaaccatac attctggtgt ttgaatccaa actctggcga taccggcgga
     4201 ctgctggaca gcagcttcaa agtttgggat gatgacaagt acaatctgtt tgaaccgtct
     4261 ctgtggcaga cacaggaatc cggaaagtat atcagcctgg atcaccagac gcctcttggt
     4321 gtaaatggaa ctggtatctc cctgtctgag tattacagta agtatgcaga cagcgaaggc
     4381 agcaatatta acggcggcac aaagggcaat acacccggcg gtacaaaacc ggtacagact
     4441 ggaacaacag aaaccggaac aaccacagaa acgaaaccgg ataccgtagt cggcgacatc
     4501 actaaggatg gcaaggtaga tgctagcgat ctggtgatcc tgctccagta tctgtgcggc
     4561 aatactgtgg acagcaaggg caaggacttc aaggcaggtg atgtgaatgg cgatggtgta
     4621 ttgaacggta tggatctggc actgtatcgc caggtattgt caaaggcaat cagcggattt
     4681 ccggaataag gcggtggtca gatgaaacgg gatgactatt tgtcctggga tgaatatttc
     4741 atgggcattg ctttgttatc agcacagcgc agcaaggaca gcaacacaca ggttggagcc
     4801 tgcattgtga accaggagca taagatcgtg tctgttgggt ataatgggat gccgacgggc
     4861 tgcaatgatg atgatatgcc ctgggaacgg gaaggcgatt ttctcaacac caaatacccc
     4921 tttgtatgcc atgcggaact caatgcgatt ctgaaccgca gcgcaggcag cttacaggga
     4981 tgcagcttgt atgtgacatt gttcccctgc aacgaatgcg caaaggcaat tattcaaagc
     5041 gggattcggg aaatcatcta ttacgacaac aaatatgctg acacggatgc tacaaaagca
     5101 tcccagcgta tgtttttact tactggtgtc acctgccgtc cgtatcatca tacagaacgg
     5161 gaaatgacgc tgcggtttta agatgtacac aattgaatcc aatagaaaag gtacgctctc
     5221 agatagaatg agagtgtgcc tttttctgtt gtttcaacac ctggttttgt gaaatatgcc
     5281 tattttcaaa aaaaacagct ttttttcaat ttccctttgt gtttttctgc aaaatatgct
     5341 ataataaaat aggtgagtta tgcaaaccgt gtaagaatct gccgacaagt ttcatacagg
     5401 tggattccaa tgaatataac ttgaatatta tttccgaaag gatgaggcat tttatgcgaa
     5461 ttcgctgtaa gccttgggca aagccggaac tggaggcttg tccgttttat atttctgatc
     5521 caaaagcctg caaaggaatg tggcaggcac aatttccgaa acagcagccg ctccgtctgg
     5581 agctgggctg cggcaagggc ggctttattt cacaggaggc tctcgcaaat ccggactata
     5641 actacattgc agttgacata aaaaacgaaa tgctggttct ggcaaaacga aagctggagg
     5701 ctgcctatgc ggcgcagcag atgccgctgg ataatgtgcg tgttatgatc gacaatatca
     5761 tgtatcttcg ggaatgtttt gcaaaagagg accgaatcga ccgtatctat atcaatttct
     5821 gtaacccctg gcctcgtggg aaacacaaaa aacgtcgtct gacacatccc cggcagctgg
     5881 tacagtatcg gatgctgctg gatggtgata tctggtttaa gacagatgat gacgaattgt
     5941 ttgaagaatc gctggaatat ttcccggagg caggctttcg catcacgact atgaccagag
     6001 atctgcacag cgaaccggaa ctgcggtatt tcgaaacaga acacgagcag atgtttgatg
     6061 aaatggggaa aaagatcaag tttttgattg cagagccgga tcctgcggtt tcagaagaat
     6121 ctttacagga attattgcag tacagcgaaa aatatagtga aaggaaacga gacaatggac
     6181 ttttccatga agaataacaa gatcgtcaat cattatttta cgaatttgaa ggaacgttct
     6241 acaattacgc cgatggaagt gattgctgac ggcacacagg cgaacgaaaa cccagccgct
     6301 tgttatcgtg gaatgggctg tgtcagcggt aataactcct cacggctttt gctggactat
     6361 aaagtagagc atccccacgc atatgaagag atcatgcggc tgttgtttca gaaagactat
     6421 ggtgcaggtt tgacgcatat taagctggaa cttggtgcgg atatcaattc ctcctccgga
     6481 acagaaccct gcaccaagcg ttcgctcaat gaaccagccg atgtcacacg tggtgcaggg
     6541 tttcagttcg ctgcggatgc gaaagcgatc aacccggata ttacggtgga ttttctgcgc
     6601 tggggtgagc cgaagtgggt cacagatgcg tttgcgatca gccaggaaca cggactgaat
     6661 gcacggtatc gctggtataa ggaaacgctg gatgctgcat atgctgtata cggattgaaa
     6721 tttgactata tcagtgctga ccagaatgag acagatacgc cggatgaggc gtggattttg
     6781 tatctgcggt atcggttgga caatgaaaaa aatgcgccgt atgattacag caagatcaag
     6841 atcattgcat cagatgaggt tggaacgaga aatattgcag agcagatggt ggacaacagt
     6901 cagttgcgca atgcggtgga tgtcattggt ctgcattata ccacgtttgg agactcttac
     6961 accaacctgc tgaacgaggc gtatggcaag gaaatttggt acagtgaagg aattgcgccg
     7021 tgcaatgtac cagaactgac agtccaggcg gatgaatctg gtctggtggg aaaaaatggc
     7081 cctatcgacg tggcaaatcg tatcatcaac agctattaca atggaaagat ggtaatgtac
     7141 gagttccagc cggcagtggc atcttattat gatggctcct gctatgcacc gaaacaactg
     7201 attcgggcgt ggaatccgtg gtccggtcac tatgcaatgg acattggatt ctggatggca
     7261 atgcacttca gccggtttgc gcaaaagggc tggatgtatg taaacggtgc ttgttatggt
     7321 gacggtgacg aagatcatgc aattgccaac acgacccata actttatgac gctcacttcg
     7381 ccggaccgca gcgagatgtc catgttcttt accaatgaaa gcgacgaagt tcgtatctat
     7441 aacgttcaga tcaaggatat gacattcgaa ccgactatgt tctccagcgt gattacgaag
     7501 ggcccgtccg gacggcaggc ttacgatgcc aactggttta agaatgggaa aaagatccga
     7561 cccgttcgga atcaccggga cacctttatc attaaagtgc cgcctcgttc aattatgacg
     7621 gttaccacac tggacacaag ctgggtggat ggtgtcaata cattcccggc agtacagccg
     7681 ccgatggcgt ccagactatc gctcccctat catgacgacc tggattacag caaggaagaa
     7741 attgcagttc gtgggtgtgc tccccggtat atgaccgatc aaggcggtgc gttcgagctg
     7801 gtggaggcag aggacggata tgcgatctgt cagaagatca cgccgcagat catgccaaca
     7861 aactggcggt tccgtggcac gccgctgccg ctcacctgtt ttggcgacga caaatggcgc
     7921 agttacagtg ctgaggcaga agtaaagctt gcaaatatgg aagagcacaa tttcgccggc
     7981 attggcattc gctataattc ggcggttacc tgtgagttta cttctatgtg cggttatacc
     8041 ggcagaattt acggagacgg cagttggaaa ctgatggaca tggaaaatgt ggcggcagag
     8101 ggaatcctcc agcacattga tcctacagtg tggaacaagc tgaaactgat ggtcctggga
     8161 aatgcagtat ttttctttat caacggcaat ttcctgacca agtatacacc gcagtgtatg
     8221 attaacagcg gtcgtgttag cctgtgcagc gattttgcat ggaatatgtt ccgcaatatt
     8281 catattgatc cggtcgctgt gcagccgctg tatgtccgca gagtggactg ttttggcgat
     8341 ggtattctgt acaatgaaaa ctgggatatc catgccatgg aaagctatca attctttaac
     8401 cgcacttcca tggaggcaaa aacaggcgct gagttctcct gtacctttga cggaacagga
     8461 attgctattg tcggtacggc acagaatgca gagtttacag tgttggtcga tggtcatacc
     8521 atgtatgaaa acttttttgt tgcggattgt ctgccgagac aggcgtgtct ggtgattgac
     8581 cagctgccag agggcaagca cagtatgcgt gtctttgtca ccagcggtca atttaagctg
     8641 gatgttctgg aaatcccgga ggcatatcca cgcatgacag ataccctgct gattccgtca
     8701 aatgttcgtt ctgccgctgc cgctgagatc cgacagcaga gcgcagttcg ggagcggcag
     8761 cgtgaggatg ctttcatgga tgcagatgca atgctgtggc atctggaaca tccgtttact
     8821 gaaccgccaa agaaacagga tgttccggca gcagatgagg acgctgtttc ttcggaagtg
     8881 gcggagactt cgacagacgt gtcggtttct gattcggatg aaaatacaag tgagacgaca
     8941 agtgatacaa cggataatga tgttcttgtt gaaacaacgg atgacagtgt aacagcaaca
     9001 gagatggaat cagaacaaac agaaccggtt tcagaggagc aggaaatcac tgcaccggag
     9061 gaaacggtga gtgcggcgca agaagttgaa cagccggtct ttgacgagga aaccagccag
     9121 tctatggcgg aaattgatgc gattttcaat cctgcaccgg aaacagaaag ttctcgtaag
     9181 gaagcggatg tggaaatgga aatgactgcg gatcaaattg agcgtattct gaatccggac
     9241 ggagcaaaaa agtatgctga cgattctgct gaatccgaac agatctgaac cgctgtatca
     9301 acagatctgc gcagcaattc gggaagaaat cgcaagcggc gctcttcagc cgaatgaaaa
     9361 gctgccgtcc aggcgaacgc tttcggcgca tttgcagacc agcattgtca ctgtgcagac
     9421 tgcatatgaa cagctggcgg cagagggata tctgtattcc cgttcccgtg ccggatatta
     9481 tgtagacccg gacgcaccgc ttttggcaca tatgccaaag gaacgagtgc tttctgaggt
     9541 acagccggta caggcagtgc cggaggatca gatcgtattt tctaccagcg gcgttgacct
     9601 ggagcagttt cccttttcca cctgggcaaa attgtccagg caggtacttt ctacaaagca
     9661 ggaaacgctg ctgcaatcac cgccggcaat gggtttatat gaactgcgtg cggcgattgc
     9721 tgcgcatttg cgggcgtttc gggatattca cgctgcgccg gaacagatct tggttggcgc
     9781 cgggacggaa tatcttttgg ggattctggt gcagctgctt ggtgcatcca ggcgctatgc
     9841 agtggaagag ccgggatact tcaaaggaaa gcgtattctg cgcagcaacg gcgcatcggt
     9901 tattgatatt ctgatggaca aaaacggatt ggatattgcg gcgctgcggc gttcaggtgc
     9961 aacagttgcg catgtgacac cgtcgcatca gttccctacc ggaatcatta tgccagtgcg
    10021 gcggcgtgcg gaattgctgg agtgggtcaa cgccgggaat acccggtttt tgatcgagga
    10081 cgattatgac agcgaactgc gctttcaagg aaaaccgttg ccgtctttgt acagcatgga
    10141 tactggcgac cgggtcatat atatgaacac gtttgccagg acgcttgctc cgtcactgcg
    10201 aatcggatat gtcgttctac cgccggcact ggcagaacag tttcagagag acttttcctt
    10261 ttactcttcc agtgtgccga gctttgaaca atatacgcta gttcactttt tgcaggacgg
    10321 ctattttgag cggcatctaa accgcatgaa gaaaacatac cggcagcggc aaaagatgct
    10381 caccgcaatg ttacaggcgc atccattggg acagtatctt tcaatacaag gagaagaggc
    10441 agggctgcac ttgttgttgg aagtgcatct tccctgtaca gaacaggaac tggtggccgc
    10501 agcgaatcaa gcgcacgtga cagtttacgg gttatccgca tactatgaga caccgcagaa
    10561 tccatcggcg tttccgcaga tcgtgctggg gtacgcctgc ctttccccag aggaattgca
    10621 gcagggtacg gagcggctgt tgaatagctg gatgcagttt ctatcataat aaatagaaga
    10681 gcgcacatct ttttagaaga tgtgcgcttt ttgcgttatg cagctgttgg gttgggtttg
    10741 ggaaatgctt ttcgcaggac aatcacaaac gagatcagca tgatgattga agtaatgacc
    10801 caggtcgtcg gaaagaccag atacatactt ttcagtgtgt tgaagtaaga gaatatggta
    10861 aaccgccata tgatgcgaat gacgcagatg cccagaatca tttcgacagc cggcaaagcg
    10921 gaatatccga ggccacgcat ggtacacgcc ggaatttcat aaacagaaca caagatctgt
    10981 aagcttaata caactaggat tcgttcgcag gaaaagcgga tttcttctgg agaagaggag
    11041 aaaataccgg acgcctggct gcggaatata acaattggat agataatcaa gctggaaaac
    11101 agaagtgcca tgccgaggct gatccagaag attttccggc agcgctgggt ctttttggcg
    11161 gcatagtttt gactggtaaa tgttgttgct gcctgtccaa atgctgtgtt gacatagtag
    11221 gcaatatact cgaagttcat ggagattgct gaaccagcag cggcagaact gccgaatgtg
    11281 ttgatggcag actgtacaaa aatgttggca acgcagaaca cagcgctttg tattgccgcc
    11341 ggaatgccga tgcgcagaat gctgctcagg tatttccact cgatacggca tttccgcaga
    11401 gacaggcgaa attcatccgg ttcccgacac agccagaata cgacaaagaa tgcacttacg
    11461 gcagtagaga tatctgtagc gagtgctact ccgatcacac taagtttgca cacaaccaca
    11521 aagaacaggt ttagtaatac atttataatg ccggataaaa tcagggcagc aagcggtctt
    11581 ttgctgtccc ctttggagcg cagtatcgcc gagccgaaat tataaacaga gagaaagggt
    11641 acgcccagga agtatacttt taagtagttg gttgccgatg tcatgatatc ctctggcgtg
    11701 tggatcagtg c
//
LOCUS       DAWENO010000054        11709 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2320_length_11709_cov_9.088811, whole genome shotgun sequence.
ACCESSION   DAWENO010000054 DAWENO010000000
VERSION     DAWENO010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_11709_cov_9.088811"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2070..2079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10392..10401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3103 a         2815 c         3277 g         2494 t
ORIGIN      
        1 cgatcctgcg caccggcacg gcaaattcca atctgaccct ggaactggga aaaaagcatt
       61 tctgcctgta tcagacaccg ttcggcgcaa tgaccatcgg cgtacaggcg aaacggctgc
      121 tctgcgaact ggaggaaaat tccggtgtgg tagagatgga atataccgta gatatgaacg
      181 caaacttcct gtccgaaaac accattacca tgcggtggga acggcagaaa gagacgaaag
      241 gaacgaagtg aaatatggca gattgtatga aaaatgcgtc tgaggcactg caccagctgc
      301 tggtggaagg tctgggacgg atcgtggcag agggaaaaat tccggcagaa cccatcccgg
      361 cattccgggt agaagttccg gcagacaagt cccacggcga ctttgcggca aatgtggcaa
      421 tggtaagcgc caagaccttc cggatggcac ctcggaaaat cgccgaactg ctgctggatg
      481 cagtttccct ggacggcacg atctttgacc gtgcagaaat cgcaggaccg ggattcatga
      541 actttttcct ccagcagaag tggtttgccg acgtgacgga aaccgttctg acagagaagg
      601 aaaactacgg caggacccag accggcgccg gcaagcgtgc gctggtggaa ttcgtttccg
      661 ccaacccgac cggaccgatg cacgtgggca acgccagagg cggcgcattg ggtgacagcc
      721 tggcagagat cctggactgg accggctggg atgtgcagcg tgaattctac gtgaacgatg
      781 ccggaaacca gatcgaaaag tttgccactt ctctggaagt tcgctatctc cagcattttg
      841 acccgtctgt ggaaatgccg gaggacgcat atcacggcgc agacatcacc gaacacgccg
      901 agaattttat cgccgagaac ggtgacaaat acgtcagcac cgatgccaag acacgccggg
      961 atgctctggt ggcatttgca ctgcccaaga acattgccgg tctggaacgt gacctgaaaa
     1021 agtaccgcat tgtctatgac aactggttcc gggaaagcac cctgcaccag aacggtcagg
     1081 taaagtgggt agtagaggaa ctgaccaagc gtggctatac ctatgagcag gagggtgctg
     1141 tctggttcaa ggcaaccgat ttcggcgcag acaaggactt cgttctggta cgtgccaacg
     1201 gcattcctac ctatgttgtg ccggatatcg cataccacta taacaagctg atggtgcgga
     1261 actacgacaa ggcaattgac atcctgggcg cagaccacca cggctatgtg cctcgtctga
     1321 aagcggcact gacagcactg ggcgcagacg caagccgttt ggatgtggta ctgatgcaga
     1381 tggtcatgct catgcgtgac ggcaaagttg tcaagctgtc caagcgcagc ggcaaggcga
     1441 tcactctggt gacgctgctg gatgagattc cgctggatgc ggcacggttc ttcttcaaca
     1501 ccagagaggc aaattcccac ttcgaatttg acctggatct ggctgtggaa cagtcctcgc
     1561 agaatccggt ttattatgta cagtacgcac acgccagaat ttgcagcctg ctgcgtgcaa
     1621 tggcagaaga gggtctgacg ctggaacagt gcgaccatgc aaacctggca cttctgacac
     1681 agccccagga actggaactg atccgtcacc tggctgcgtt cccgtctgaa ctggatgccg
     1741 cagcaaaggc gtatgatccg tctaaggtca ccaagtatgc cacagagctg gcaactttgt
     1801 ttcacaagtt ctatgacgtt tgcagcatca agtatgcaga gacaccggaa ctgaaacagg
     1861 ctcgtctgct gctggctgag gcggctcgcc agggcattga aaatgcgctg cgcattctga
     1921 aaatcacctg tccggagaag atgtgatcgc cggaaaatac cggaatcatt acaaacaggt
     1981 tacagcaaaa atcagatcat ttctgctgaa tttgtgcaaa aacacgagaa aagcaacgta
     2041 atactcgaaa taccccataa agtttaacgn nnnnnnnnnt taacgacgta ttcacaaatg
     2101 ccgtcgaaga atgtgttaca atttgtaata tattgagcag gcgagttgca tttgtgcttg
     2161 cgaaaaaagc agcactgctg aaaaggaaag gaagttctat atgtcgaaca gattatttca
     2221 aggactggtt caccagatgc gggataccat cgacgcaacg gttggtgtgg tagatgatac
     2281 cgcaaccatc atcgcctgca gcgatctcac caaggtggga acgaccaacg agttcgtttc
     2341 tctggatctc agcgatgtac acgatatttt catccgtgac ggctatacct ataagccgtt
     2401 cggctcccgt gcaaagccgg agtatgccgt gttcgttgag ggcatggatg acactgctgc
     2461 aaagtatgcc aacatcctgg cgatctccct gttcaatatc aaacagtact atgatgaaaa
     2521 gtacgatcgc aacaacttca tcaaaaacgt ggtactggat aacgtactgc cgggcgatat
     2581 tgtcatcaag gctcgtgagc tgcacttcaa cgcagaagtc agccgtgtgg ttctgctggt
     2641 gcgcatcgta tccgcaaacg atgtttctgc atacgatgtc atccagaatc tgttcccgga
     2701 caagaataag gactttgtct ttaccatttc cgagacagat atcgttctgg tcaaggaaat
     2761 caagggtgca gtggattcca aggacctgga aaagctggca cgctccattg cagatacact
     2821 gagcagcgag ttttacactc gtgtcaatgt tggtatcggc acgatcgtta ccggggtaaa
     2881 ggaactgtcc cgttccttca aggaagcaca gatggcactg gaagtcggaa aggtattcga
     2941 taccgataag gcaatcgtca gctacgagaa cctgggtatc gcccgtctga tctaccatct
     3001 gccgacaacg ctgtgcgaga cattcctgca cgaagtgttc aagcgtggca gcatcgaatc
     3061 gctggatcac gagaccctct tcaccatcca gaagttcttc gagaacaatc tgaatgtatc
     3121 tgagacttcc cgtaagctgt tcgtacaccg taatacactg gtgtaccgcc tggagaagat
     3181 taagaagctg accggtctgg atctgcgtga attcgaccac gcaatcattt tcaagatcgc
     3241 actgatggta aagaagtacc tctctgcgaa tccggcgaaa ttctaattga atcctagaac
     3301 agcgttctgc tgttcttatc acaaagaccg ccggtgcagc gtctggcgtt tttgcatcgg
     3361 cggttgcttt gcgcttatgg tatagattgg cgaaaattgg atgtctgcga tcaactgaga
     3421 gcctgccgcc gggtgcggcg gactcgaaaa cggaaatgac ggacaagctt gtctgtcagc
     3481 accaaaagaa aggatgtgtg tgaactatgg ttgaactgaa ggatgtctct gtgacatatt
     3541 ccagcggcgt agacgcactg cacaatgtca acctgaaaat caatgacgga gactttgcct
     3601 ttgtggttgg cgcttccggt gccggtaaaa gtaccatgat caagctgatc ctgaaagaga
     3661 tcaatccgac cagcggttcg atctatgtta atgggtataa tttgaataaa ctgcggcggc
     3721 ggcgtatccc ggcgctgcgg cgtaccatcg ggtttgtgtt ccaggacttc cggctgatcc
     3781 cgtccatgac cgtatacgaa aacgtggcat tcgtgctgcg tgtaattgat gcacctacga
     3841 agtatatccg aaagcgtgta ccgtatgtaa ttggtctggt gggcttgcag gataagacga
     3901 acgcttatcc agacgagctt tccggcggcg aaaagcagcg tgtggcgatt gcccgtgcgc
     3961 tggtaaacga tccgcagctt atcattgcgg acgagccgac cggcaacatc gacccggaac
     4021 tgtcctacga gatcgtagaa ctgctccagg gcatcaacga tatcggtact acaattctga
     4081 tggttaccca tgaacatagt ctggtgcgcc agtttggcgg acggattatc aatatcgatc
     4141 agggacagat cgtctttgac gaagtgattg gagggaatct gatccatgaa agttagtggt
     4201 ttcaagtatc tggttggaca aggcgtggag aacatctgga aaaaccggat gatggcattt
     4261 gcttctttct gtgtcctgct ggtttctctg ttgctggtgg gaatgtcggt gctgttttac
     4321 atgaatctga catccatgat cggcggcgta gaggacaaaa acgaagtcat tatctatatg
     4381 gatgaaggta cgaccgacca gcagcttgca gattttcaat cagaactttc ccagattaaa
     4441 aatatttcca gcatcacctt ttattccaaa gaacaggcgt ttgaagatct gaaaaacagt
     4501 atgtctgatt atgaggtgct gttcgattct ctgggggatg acaacccgct gatcgacggc
     4561 taccgcatcc gcatcgacga tatttcgcag atctccacca ccatcgccca gatcgagcag
     4621 ctggatcaca tctactcgat ccgtgcgcct atggactttg tcaatgttct gacagaagtc
     4681 cggaagatcg tcaccgtggt atttggtgtc attattgcgg cgctgatcgt gatttccgtg
     4741 gtcatcgttt ccaacgccac aaaggcgagc gtgttcgcac gccgggaaga aattcagatc
     4801 atgaagtatg ttggtgcgac caacgccttt attcgtatcc cgttctttgt agagggtatg
     4861 attaccggtt tgtttgccgg agcagtagcg ttggcgatca cctggttcgg atatgattcc
     4921 ctggtgggac tgctcaccgg acaggtagac ttcctgtcgg tcattggcat gggcagcatc
     4981 attccgttcc gtgcgctggc atggaaggtt gcgttggcat acctggtgac gggtacgctg
     5041 ttcggcgcat tcggcagcat gatttcgatg cggaaacatc tgaacgtata aatggcacgt
     5101 tctgaggagg ctaaatatac aatgagcaaa aagaaacaca tgggtctgcg ctttaccagt
     5161 gcggcgctgt gtctgagtat tctcgccgca ggcgttgcgg caaactggac accggtgaaa
     5221 gatatcccgg tatccgcaaa aacactggca gaattgcagg aagagcgaaa gagcaatgag
     5281 gcgaagatcg ccgaaaagca gaaacagctg gattccctgc agaccaattt gaaggaaaaa
     5341 gaagcatacc aaaagacttt gcaggagaag atcaccttgc agcaggagaa tctggacatc
     5401 gtttcagagg aactggaccg cattgccaag tcgatcgatg agactacaca gcagatttct
     5461 caggcagagg atgatatcaa ggtaatggag gcagatatcg ccatcggact ggacgagttc
     5521 aagatgcgtc tccgtgccat gtacgttcag ggtaacgaca gcctggcatc tgcgctggtt
     5581 ggcgcaacgg acttctacga tctgctttcc aagtatgagc tgatgtcccg tgtggcaaac
     5641 cacgacaatg aactggtcaa cgatctgaag gacaagctgg agacctgcaa cgaaaaaaag
     5701 gcggaactgg aaacagaaaa gtccgacctg gaacagcagc agtctgacca gcagcagaaa
     5761 aaggatgagt tcaagactgc catcgaagat ctccagcaga cttatgcgga gaccgacgag
     5821 gcaaaggaac agatggagcg tcagaaagaa cagctgaacg gcgatgttgc aactctggag
     5881 aagaacaaca aggaactgga tgccgaggaa caggatatca aggcggcaat cgctgcggcg
     5941 gctgaggcag aaaagaagaa aaaggctgcg gaggcagcag ctgccgaaga ggcaaagaaa
     6001 aagctggctg cacagcagga ggcggaaaag aagaatcagt ccacttccaa tagcagtaat
     6061 aacagttcca gcagtaattc ttccaagagc gacagcagca gttccaacag cagctcgaac
     6121 aatagttcca gcaactcctc ttccagcgac aacagttcat ccggcagcga tgacagcggc
     6181 agcacagttg caccgtccgg cggcggattc acctggccgt gtccgggatt ctataccgtt
     6241 tccagtacat tcggcaaccg ctggggtaca acacatggcg ctattgatat tgccggcggc
     6301 aatgccggtg cggcaatcgt ggcagcgaag agcggcacag tcgttcgtgt cgtaaccgga
     6361 tgctctcata actatccgaa gagcggcagc tgcggctgcg gcggcggata cggcaactat
     6421 gtcattatcc agcacgacgg tacatattct acattgtacg gtcacatggc atctgtatcg
     6481 gtttctgtgg gacagtctgt cagtgccggt cagaccattg gctatgtagg aagcaccggc
     6541 ttctccaccg gtttccacct gcactttgaa gtgcgtgtca atggcacaaa ggttgacccg
     6601 atgaaatatc tctaatcaat cgtaaatact aggaaaaggg caggggtgtt cagtttggac
     6661 cctctgcctt ttttggaaag aaaggcgatg tcatatgaaa gagaaacaaa acgaaagctt
     6721 tgagaccagt cagaaagaca acactgtccc ggtgcggaaa tccacactga ttctgctgtt
     6781 ctgtctgtgc ggtgttcttg ctgccggaac aggtgtgggg atctatcgga gctaccgcag
     6841 ccaggacaca taccgggaac tggatcagct ggcgaaaact gtcaagcaga actactatac
     6901 ggatgtggac gaaaacgacg tgatgcaggg cgctatgaaa ggatatgttg ccggtctgga
     6961 cgatccgtat tcccagtata tgacagacga ggagtacagc gcctatcaga cggacgaggc
     7021 agggcagacc atcggcatcg gcgtaaccgt gcagcagaat gaggacggct acctccaggt
     7081 ggtatctgtc agcgacgatt ctccggcaaa ggatgccggg atacaggcgg acgatgtgat
     7141 tctcaaggtc ggggaagatg atgtggcaga actgggctat gccggcgcag tggcggaagt
     7201 gcgcggcgag gctggcacta ctgttacgct gaccattcag cgtggcgatc agaccaagga
     7261 actggaagtg gagcgccgta gcatggatgt gactacggca cagggcaaga tgctggatgg
     7321 gaatatcggt tatatccgga tcagcgcttt caagggcaat actccagagc agttccaggc
     7381 gatttttgac cagttggtgc aggacggcgc aaaggctctg gtgtttgacc tccgggatga
     7441 cggaggcgga ctggtggatg cgctggaaca gattctggat ccgctgctgc cggagggcga
     7501 tattgccatc gcaacctatc gggacggcac gaccaagacg ctggtaaaat ccgatgcgga
     7561 ggagtgcaat ctgcctatga cggtaatcgt caacggcaac actgccagcg ccgcagagct
     7621 gttcacggca tcgctgcacg acttcggcaa ggcatccatc gtgggaacgc agaccttcgg
     7681 caagggcatt atgcaggtga cgcagaccat gcccggcggc ggtgcgctga cgctgacggt
     7741 tgcaacctat cagaccacaa agggggaatg ctaccacaaa gtgggcatta ccccggatga
     7801 aatcgtagaa ccgggcgagg atgcgataga ctacgataat cccgacccgg agaaagatcc
     7861 gcagctgaaa aaggcaatcg aaatggtgac acagtgactg taaatctttt ttgaacagac
     7921 atccgcttgt cccagctctg tgatataatg aaagcatcac cacaagaaaa agcaaaggag
     7981 acgacaacaa tggagtcaga aaaaaacagc cggatgctgg aactcttttt tcgggtgctg
     8041 cgtggagaga cggtttccgt caaggcagtt gccgaagaat atggtgtgtc caccaaaagc
     8101 gtgggcagag atattgcaaa gatacaggag ttcctcacgg atcaccgaga tctgatgcag
     8161 caggcggaac tgatctattc ctataaggat aaggcgtatc acctcagctg tgacgagttc
     8221 ctgaaaaaca aggagctgtt cgcactggta aaagtgatgc tgggcagccg ctgcttttcg
     8281 aaagaggacg tgctgcgcat gattgccaag ctgaaacggt ttacgaccat ccaggatcgg
     8341 gaaaagttcg aaaagctggt acgcaaggaa gtctatcact accacgaggt gaaatcggac
     8401 tgcaatgctc tcatcgataa tctgtgggac ctgatccaga tgatcgacga aaagcgcctg
     8461 gtgaccatca cgtattacaa gatgaaccgg gaaaacatca agcggaagat caagccggtg
     8521 tcgctgatgt tcagcgagta ttacttttac ctcatcgcct acgaggcgga tgatgagaag
     8581 tatctgccga agtttttccg ggtggatcgc attgtggcaa tgacggagca tcgggagcgg
     8641 ttcacactgg aacgaaagta tgactttgac gagggcgacc tccgggagaa aaaccagttt
     8701 ctgtttccgg gcaagacgga gaagatacgc ttttcctttt ccggactgtc tgtgcaggca
     8761 attctcgacc ggctgcccac ggcaaaggtg gtggagcaga acggcaatac cagcatgatc
     8821 gaggcagagg tcaaccacgg cagaggcatt gtaatgtacc tgctttccca gggcgcatgg
     8881 gtcaaggtac tgtcacccca gagcctggtg gaggagatgc aggaggagct gcggcagatg
     8941 tgcagcgcct atgacatctg agcaaattgg ataacatgga ttcgaaaaga cttgtgtgac
     9001 ataacgccgc acaggtcttt ttttggtatg cagcaccttt cggaatgttg tcatgaagtt
     9061 actacttttg tcatgaaaaa tggtattatt tctgtgcaga gtgtgctaaa ataaaaacaa
     9121 tcaaaccgat gaaaaaacag tccgttttgg tgcagttcct tcggaacgca tgaaacggca
     9181 taagaccaaa taagaaagtg aggaaaaaga atgcgaatga aagtattccg gcgctgtctg
     9241 gcagttttct gcgcagcggt gctgagtgtc ggcagtctgg cagcaatgcc ggcggctgcc
     9301 gagacaaaaa acctaatcac aaactccaca tttgacagcg gtacaacggg ctgggggaca
     9361 taccaggcga gcggcggcag cgccacgctt tctgcccaga atggcaagct agcgctccag
     9421 atcaattctg tggggaagct gaattattcc gtgcagtgca attatgatgt gattccgctc
     9481 tacaaaaacg gcgtgtatca tgtcagctat gagatctctt ctaccacaga ccgctatgtt
     9541 gaggcgatga tccagcagaa cggcggcaca taccaggcgt atacctggaa aggcattgat
     9601 cttacctctg agccgcagac catcgattat gagtttacca tgaaacagga cacagatatc
     9661 atgtccaagc tggtgttcaa ctgcggactg gaaaacgggg aggatctcga cccgcacacc
     9721 atctacctgg acaatgtcaa ggtagaactg gtggacgatt ccaaggtgga atatgacgat
     9781 atcctgccct atgcaccgtc gatcatcacc gaccaggtgg gctatcagcc ggaggaaacc
     9841 aaaacggcag tattccggga tgtgacaaat gagaccagtt tttccgttgt caatcaggac
     9901 agcggcaagg tcgtatacac cggcgaactt tccgcatcca agttcaattc catggcgaac
     9961 gaaaccgact ggacaggaga tttctccgca atcacagagc cgggaactta ttacatcacc
    10021 tgcggcggac tggatcagtc ctatgccttt accatcggcg aagatgtgta taatggcttg
    10081 ctagatgata cggtgcggat gctctacctc cagcgctgcg gcacagaaat agaggatgac
    10141 acattcggtc acgccgcctg tcacaacacg ctggcgactg tttatcacac cagcgacaag
    10201 atagacgtca gcggtggctg gcacgatgcc ggagactatg gcagatacgt ggtgccgggt
    10261 gcaaaatccg tggcagacct gctcattgcc tacgggcaga acccggggat gttcagcgac
    10321 agcatcggca ttccggagag cggaaacggc gtcccggatg tgctggacga ggcacgctat
    10381 gaactggaat gnnnnnnnnn ntgccggaaa acgaaacgga tgaactgatc gtgacaccgg
    10441 tttccactac ggctactgca gacttctgcg ccgcaatggc gatggcatac gagtattatc
    10501 agaatgtgga tcagaccttc gcagagacct gtctggatgc ggcaaagaag gcgtgggctt
    10561 tcctggaaaa gaatccgaac tttattttct ccaatccgtc cgatatcaca accggtgatt
    10621 acggcgatag ttttgacaca gatgaacggt actgggcagc agcacagatg tggcgtgcca
    10681 ccggggagga aacctatcgt tctgctctgg aaaagatgag cgtacagacc ggcatggact
    10741 ggatggaaat gggcgattac ggcaacttcg ccatcctcac catgaaaggc gtggataccg
    10801 attccacgat ctatacccgt gcaaagcgtt cgattctgca gtccgcagac aaggcaaaat
    10861 ccctggcgca gaatgacgca tacggcgtat cagtggcgaa atataactgg ggcagcaaca
    10921 tgggcgttgc cacatccggc atgatcctga acctggcata ccagctgaca gaagattctt
    10981 cttatctgga gacatcccgg agcaacctgc actatctgct gggcagcaat gccgtgggcg
    11041 agtgctttgt taccggttac ggcacagttt ctccggagca tccgcaccac agaccgtcta
    11101 tggcgaagaa ccaggcgatg aagggaatgc tggtcggcgg cgtgaactcc ggtctggagg
    11161 acagcgctgc caaggcgtac tgcgccaact ctccggcggc gaagtgctat gtggacaact
    11221 gggaaagcta ttccaccaac gagatcacca tttattggaa ctccccgctg acttgtctgc
    11281 tggcaatgag cggaaagcac agcgccgata caccggagga ggatatcctg ccgggcgatg
    11341 tgaacctgga caaggcagtg aatacggcag acatcgtact gctgcaaaag tatctgctgg
    11401 gtgcggagac gctgacaaag gcacaggcga aaaatgccga tgttctggcg gatgagactg
    11461 taaacggttt tgatctgact gtactgcggc agatgctgac cgaacagaat gacgattcgg
    11521 acaagggcaa cgaaccgagt tccgatgcag atgttttggc tgatttccgc aagggtgcga
    11581 ctagtctgtt tgaggcatct gacggttgga caaacggcaa tccttttgac tgcggttgga
    11641 caaagaacaa cacttccttt gacaacggcg tgctgaatct gaccatcgac aaggatactt
    11701 ccgggaagt
//
LOCUS       DAWENO010000055        11686 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2323_length_11686_cov_14.253461, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000055 DAWENO010000000
VERSION     DAWENO010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_11686_cov_14.253461"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2846 a         3078 c         3284 g         2478 t
ORIGIN      
        1 gtttatcgtg atgtatattc tgaacaagct gatcggcaag gtcctcagca agtatatgga
       61 ctaaggagtg tgcagtatgg caaaaaaaga aaaggtatac ggcacacacc tgaaggtatc
      121 gaccaagcgt gccgggcata agatcggcgg cacggtgggg atttttgtgt tcctgctggt
      181 gctggcattg ttcatggcgc tgcccatcta tctggcagtg gtcatgtcgc tgaaaccggt
      241 gcaggaactg ttcgtgttcc cgccgaaact gtatgtcatt gatcccaccc tgtccaacta
      301 cagcgccatg ttccggctct gctccgacct gtgggtgccg ttttcccgtt atgtatttaa
      361 tagtatcttt gtcaccgtat ccgttactgt gtgccaggtc atcttctccg ccatggcggc
      421 gttctgtctg gcaaaggtgc gttttcccgg tgcaaaggcg atcaatgcga tcatcgtcgt
      481 tgcccttttg tatcagagca acgtcatcta tatcatgcag tatatggtca tggcaaagat
      541 gggcatgatc gacacctact gggcgctgat cctgccgtct gtggctgcgc cgatgaacct
      601 gtttctgatg cgccagtcca tgacccaggt acccgatgcc atgatcgagg cggcgaaggt
      661 ggacggcgca ggaatgttcc gcatctgctg gcaggtggtc atgccgaacc agaagccggc
      721 gctgatgact gtcatcatct tctcgttcca ggcggcatgg aacatccagg gcggtacgct
      781 ggtattcagc gaatccctca agaccctgcc caccgtggtg cagcaggcag catctgccgg
      841 tctggcgaga gccggcgtgg caatggcggc cgccgtgttc atgctggtgc cgccgatcgt
      901 catcttcatg ctggcacagc ggcaggtcat cgagaccatg gcacattccg gaatcaagga
      961 ctaagggagg gtgaagattt tgcagaaaaa ggcaaaccat ctggcggcgg cagttttttc
     1021 cgcactgatg ctgacggcag ccataggcgc tccggcggtt tcggcggaac attacaacgt
     1081 ctataactac gactgctggg gcgaggcggt tccggcgcag gcaggctatg cggcacagcg
     1141 tgccgtgagc ggccaggatc tgggctgcgg cgcattcaac accccgtccg atctgttccg
     1201 ggactgggag gatcgctttt atattgcaga cagcggaaac aaccgcatcg ttgtcacaga
     1261 cggcgatttt tcaaaggcga ccagaattta tgacaagctg aaaaccgagg acggcgagac
     1321 cacgctgaaa gatccggagg gcatctatgt gtctcaggag acccagtgta tgtacatcgc
     1381 agacaccgga aactcccggc tgctggtgtg cgatctggac ggcaatgtgc agatggaact
     1441 gaaacggccg gactcgaccc tgtatgaaaa tgagaccttt aagccccaga aagtcgttgc
     1501 ggacaaagcc ggcaacatct atatggtgct gaacaacatc accaacggtg cggcaatgtt
     1561 caacagcgac ggcgaattcc agggctattt cggtgcgaac tccgtggacg caaccgcaga
     1621 ggtggtggcg aactacttct ggaacctgtt cgccaccgat gagatgcgtg ccaactcgtc
     1681 ccgtaacgtg gcggcaggca tcaccagctt tgacgttgac gaagagggct ttatctatac
     1741 ggtaacacag tcctcttcct ctgagacgga ccgtgtgaaa aaggtgaacc ctgccggata
     1801 taacctgttc acttcgctgg atgtgacatt cggcgacctg gactcggtct atgactccac
     1861 cgccaacgag aactacacca cccaaatggt ggacatcgac atcgacgacg acggacgcat
     1921 caactgcctg gatctggaga ccggacgagt gttccagtac gacgaggacg gcaatctgct
     1981 gttcattctg ggaacgactg ccgaccagat cggcggcttt tccatgaagg tctccgccgt
     2041 ggagactatg ggaaagaaca tctacgtttc cgatgccatg aagaacaccg tcaccatctt
     2101 caccgagacg gaattcggcg gcatcgtcca tgatgcggta tccctgtaca atgccggcta
     2161 ttatgcggag gcgctggagc cgtggcgtga ggtgctgaaa cgggacggca actcccagat
     2221 ggcatacatc ggcatctcgt cggcgcttta taatgagggc gactatgccg gggcaatgaa
     2281 atatgccaag ctggcgcagt cccgcaattt gtacgataag gcattcgagg gctatcgctc
     2341 tgaatggctg aacaagaatt tcacctggat catcctggtg gttgtggtgc tgatcgctgc
     2401 ggcggtattc ttccacttcc gcaacaaaaa gaagaaaaag aaccagccga agaatctggt
     2461 tgaaatgctg cacgagggag aggaggagtg atgctgctat gatggatctg acacaaaagc
     2521 aatgggtcaa gcacagtgtg ttccacccgt ttgagggctt tgaggatctg cgctggaaaa
     2581 aaggcggctc ggtgctgtac gccaccatcg tggtgctgct ctggttcgtg gcacagatca
     2641 tccacgataa cctggtgggc tatcaattcg ccgttaccaa taccaagatg ttcagcatcg
     2701 tcccgtatat cgtccagacg atcgccgtat ttctggtatt cgtcatcggc aactggtcga
     2761 tctgcaccct gctggacggc gagggcacga tcaagaaaat ctatatctac agtgcctatt
     2821 cgctgatacc ctacgttgcc ggactgtata tccggacgat cttgtcccat gtactgattc
     2881 aggacgaggt gatcttcatc acctgcgtca cggtaatcag tacggcatgg tctgtgctcc
     2941 tgatgttcaa tgccattaag gcggtgcatc agtattcgat cggcaagacc attctggcac
     3001 tgctgctcac ctttgtagca atgcttatca ttctgatatt gctggtgttg ctggtggcat
     3061 tgttccagca ggtatacgtg ttcatttcct ccgtctacac ggagatcacc tatcgggtaa
     3121 gagtatagaa aggacggtgg aacaaccttg aagaattgga aaaaatgggc tgccggactt
     3181 tttgcgctga gcctttgcct gacgtcggtc tctctgccgg ctgcggcgga gggggaagag
     3241 gatatcgccc tcatcgctga cacctcagaa gagacaccgg tggcagacgg cacacccgat
     3301 gagaccgccg gggacggcga ggcggatgcc gaagaagagg cgacccgcac ggagacccag
     3361 gaagaaatcg aaattacggc agagcaggtc acccagtata tgcagaagaa gaactcctgc
     3421 gacggcatca cattctacta ccgtccggag gactatgagg ataccatttc cgacgaggat
     3481 gtggtggatc tgctggacga catcgagctg gcaggcatcg acgatgccac cggcgaggtg
     3541 gtctgcaccc tggaagagga cagcgacaac agcgacttcg tggtattttt aagcccggag
     3601 agccgctggc tggtgtacat ggacccggaa tataccaagg tcaccatggt gcgccagatc
     3661 gtttcttccc tggacaacga gctgctgttc cgctcccggg acaacaagac cctggagctg
     3721 tacaacaagg actacgacga agtggaacgc agctatacca cagacggcaa ggcgaaggac
     3781 ggcaaggtca cttataccaa cgaggacggc tggcaggtgg tgctggcaga tacctatgat
     3841 gccgtgatct cctctgcacg gttcgtcacg gaaaacgaca agctggcgct gtatgtggac
     3901 gatgacaccg cagtcatcgg actctacgac aaggcgaaag ataaaatgtg gtggtctacg
     3961 cccgaaaacg tgggacacga caagaccgca accaatacca ttgtagagga cctgtcctct
     4021 tcgctgaaaa tggtctacgg cgaaccggat gcccggagta ctaccaatat gcgctccagg
     4081 ggcgacgcca aaattaaggt caaggacaag agcagcggcg tgaagatcac ctattccttc
     4141 aaaaaggcag gcatcaccgt tccggtgacc tacaccctgg aggatgacta cctggaggca
     4201 aagatcgaca ctgccgacat cgaggaagaa gatacctcac agtccggaaa gctggtcaca
     4261 tccctttccg tgctcagcag cttcggcgca gcgtcctctg cggacacggg ctatttcgtg
     4321 atcccggacg gcagcggcgc actaatccgt ttcaacaacg ggaagaaaac ggcgaaatcc
     4381 tataccggct atgtgtacgg ttcggatgtc accgccgtgg cacagaccga gccggctgtg
     4441 actgagcagg tctatctgcc catgtacggc atcgtcaacg gcgacaacgc catgatggtg
     4501 gtctgcaccg agggcgactc caacgccaag ctgaccgcaa gcgtcagcgg acagtccaag
     4561 agcagtttca acatctgcgg ctttgacttc accgtccggg attcggatac ctactatatg
     4621 tccggcgaca acagcacggc gctcaccgtg tttgaggacg gcgacatgaa aaccgatacg
     4681 ctggcggtgc ggtattatcc gctggagacc gaggacaccc cggattacac cgatgtggct
     4741 gaggcatacc ggaactacct taccgaagag gcaggggtga ctgatacggc agaggacacc
     4801 gaccccggac tgtacctcaa cttctacggc ggcaccataa aggagaaatc cgtgctgggc
     4861 gtgccggtga aaatgaaaac agcgctcacc agctttgagc aggcggagca gattttgcag
     4921 gatctgtcgg acggcggcgc cgagaatatg aaggtgcagt attacaactg gaccaacgcc
     4981 ggcatttccg gcaaggtgga tctgaaggca aaggctgccg gctgcctggg cggaaacagc
     5041 gactggaagg cgctgcagag ctatgcggat tccaacggcg tgactctgta tccggcaacg
     5101 gacaatgaga cgttcaagtc cggcaacggg tactacacct ttaccgatac taccgtccgc
     5161 atctccggtt cctatgcccg catctatgac tacaacctgg catacggcac acagagtacg
     5221 gcaaacaagc ccctgtccct gctgtctccg gcgaccttca ccgagatcgc tgaaaagctc
     5281 accgggaact atcaggataa atccctgtcc cgtgtctccc tgggcagtct gaccacagcc
     5341 ctctacgggg actacggcaa gcaggagatc tcacgggata aggcacagca gctgctggaa
     5401 gagtcctatc agaagatcac agacggagac atcaccctgc tggcagacgg ggcaaacgcc
     5461 tatgcgctgc cctatgttca ggagatcacc gatgtgccgc tgcagtccag cggattcgat
     5521 gtgtttgatg aggacatccc gttctatcag atcgtgatgc acggactgaa atcctatgcc
     5581 ggcagcgccg tgaatgcctc ggcaacgccg gaagagaccg tgctgctctc cattgcatcc
     5641 ggcagcagcc tgcacttcga catgatcggc gaagagacca gcacgctgaa agacactgtg
     5701 ctggacggtc tctattatgc cagcgccgaa agctggaccg attatgcggc acagagctat
     5761 gcgttctcca aggcggtgct ttccggactg ggcgaccaga ccatcaccgg ctatgagaga
     5821 aacggcgatg tcattaccac cacatatgca aacggcacgg tggtagaaac cgacctgtcg
     5881 aaacagatcg tcaccgtaga cggcaaggct tatgccatgg cggattatgt ggaagaaggg
     5941 agctggaacg aagcatgaaa atggcatatg aaaaacgaaa gagtatgtac ggctactgct
     6001 tcattgcgct gtggtttatc ggcgcactca tattctttct gatcccggtg gtggaatccc
     6061 tgttctactc gttccgggaa gtcagcccgg acaccggcgg catggtcggt ccgtttgtgg
     6121 gactggacaa ctataactat gcgttcaacg tagacccgaa ctaccgccag tacctggtca
     6181 aggtgctgaa agacaccgcc tggaaaacgc cgctgattct ggtgttctcc ctgttcgtgg
     6241 cagtcattct gaaccagaag ttcaagggac ggaccttctc ccgtgccgtg ttcttcctcc
     6301 cggtcatcat tgccaccggt ccggtgtaca acatcatcaa cggcaacctg caaagcaccg
     6361 gcaacagcga tgccagccag ttttccacca tgttctccac tgacctgctg ggtcagctga
     6421 tggagttcct gggcatctac ggcatctccg acagtatgca gaccaccatt gaaacggtgt
     6481 cggacaacat cttcggcatc gtctggaact ccggcatcca gattctgatc ttcctggcgg
     6541 cgctccagaa cattccggtc tccgccaagg aggcggcgca gatggagggt gcgacggcgt
     6601 gggaatactt ctggaagatc accctgccct acgtcagccc catgatcctg gcgtgcttta
     6661 tctttaccat cattgattcc tttaccgacc cgaacaacgt ggtcatgggt cgtatcctcg
     6721 acctgcaaag cgactggcag tacggcccgg catcggcaat ggcgtgggta tacttcgcca
     6781 ttgtgctggc ggcggtgggt atcattaccg caatcctcaa caagttcatt tactacgaag
     6841 tagactaaaa aggagatgtc agttatggaa acaacaaaaa ataagccgct ggcatccccg
     6901 aaagcggatg cccaggtggg caacggcatg agcaagcgtg aggctcggaa gatccgcatg
     6961 aagagcatga acaaatccga gatcgcctat atgcgccgga aacgggtgtt tgaggcagtc
     7021 tggccgtttt tccggttctt catccttttc ggactctgct ttgtcatcct ctatccgctg
     7081 atctatatga tcagctgtgc gttccgtgac cccacggaca tgagcgaccc caccgttatg
     7141 tggattcctc gtcacctcac ctggagcgtt atcaaggata ctgccaatgt catggacatc
     7201 tggagcacgc tgaaaaccac gctcctgctg aatgtgggct gctcgctggt acaggtggca
     7261 tcctgcgcac tggcaggtta tggtttcgcc cggttcaagt tcaagggaaa gggcattctc
     7321 ttcggcatcg tcgtcatgat gatcctggtg ccgtcgcaga tcatctccat tccccagtac
     7381 atgatgttcc ggaactttgg tttcggcagc ctgtcggtaa acctcatcaa ctccaagctc
     7441 tgtatgtacc tgccggcagc catgggcaac ggcatccgtg ccggactgat gatcttcatt
     7501 ttccgtcagt tcttcaaggg tctgcccaag gagctggaag atgccgcata tctggacggc
     7561 tgcggtccgc tccgcacctt cgtccaggtc atgatcccca acgcatcctc ctcgtttctg
     7621 accgtgttcc tgttctccgt agtctggtac tggaatgact actatgtcag ctccaccttc
     7681 ttcaccaaga accagaccat tgcgctgatg ctgaaaaatc tgagcaccct gctttcccag
     7741 cagctgttca acaacgcaga tgtcagcccc agagaacaga tcgtctggat ggaggcaggc
     7801 tgtctgatcg caattctgcc cattctgatt atgtatgtgt tcctccagaa gtatttcacc
     7861 gaaggtatcg aacgctccgg tctggttggt taacatttcg aaataagatg tacccggtct
     7921 tttgcagacg ggttctgaaa ggcgctttcc gctgggagag cgccttttgg tttttctatc
     7981 tatccttata tagaaagaga ccaaatgttc cgcacccctt ttcccgaatc tgcatacgtt
     8041 aaggatgtaa gggcagcgct gtgtgctgtc cggaaatcta tttttcagga atgccttgcg
     8101 gcagtgcttt caacaggttt tccacgtaga aatgagtgga aaagttgaaa acctgttgaa
     8161 aacatcactt tttcactgtg gaaaaccgca gaaatgcgga gcggagggaa aaactatgac
     8221 atccgacaat gtgattcttg tggcaggcgg agacctgcgg cagcagtacg ccgcccggcg
     8281 gctgcggcaa aagaccgggg cagacgtatg gacgctggga cttccggcgc agtccgggct
     8341 gcacagtgcg gaaacgctgg aggaagtgcc ggcgtatgac gttctggtgc tgcctgttcc
     8401 ggcgagtccg gacggaacaa ctgtcccggc accgttcgga aaggcggcgc tcccgctgga
     8461 gacgctggcg gcgcacggca agcccggggc gctggtgctg ggcggagcgt gcggcggcat
     8521 tcttccgtgg ctggaatccc ggggactggc ggcggcggac tatctccgcc gggaggaact
     8581 ctgcctggcg aatgccgtgc cgtctgcgga gggcgccatc cagatcgcca tggaagagac
     8641 cgcccggacg ctgcacggca cggcagcgct ggtgatcggc tacggcagga tcggcatggc
     8701 gcttgcgcca cggctgcgtg gcctggggat ggatgtgacc gtctgcgcca gacggtgcga
     8761 gacccgggcg ctggcagaga tgcagggatt cgctgccgtg ccgatggaac agctcccggc
     8821 tgcggcaaag gcagccccgg tgatcttcaa caccgtcccg gcgctggtgc tggatgaaac
     8881 tgtgctgaaa gggctgccgc aggaaacctt ggtgatcgac ctggcgtccc gtcccggcgg
     8941 cacggacttc gaggcggcaa aacgcctgag aacacgtgtg gtgtgggcat tgtcccttcc
     9001 gccggaaatg cggcgttgac acacgctgga aaggggttcc aggcgtcaaa aatgttgaaa
     9061 agtcggtgga aagactgttg aattgagaat tccaggctga aaattgtgaa aaagcgacaa
     9121 aaacggggat aatatccagc aaagaatcta aagaaaattg agtcaatggg cttgacacgg
     9181 gcgcaataat atgttaaaat acgtatgtaa attgtttacg agggcttttt ataggtgctt
     9241 gtaagcatga agattgccgc agcgtgcgtc tttcgggtat ggaagcggga ttgcccggat
     9301 gcgaatgcga ctgttggggt tggatgaatc acatccatgc agacggcaat gaaactcaac
     9361 taaaagagag gtaaagagaa atgaagaaga cattcaacag acttcttgct gcaactgtcg
     9421 caattccggt tgcactgggt cagattcttg caatttctgc aaacgctgcg gaagctccgg
     9481 cagcaatgca ggtcaccgca gatcagctgc tgaaggtaaa cccggagact ggtttcccga
     9541 ctgacgctga gggcgatacc attgaataca cagtagagtc cgactggaac actgtactgg
     9601 gtaagaaggt agagtctgct gcaaacggca agaccatcac cctgaatgct aaggatctgg
     9661 ttgctggttt caacaccaac aactactacg ctggactgct gaaggacatc gtaaacgctt
     9721 ctgaaaatcc gaccgctacg gtagaaaacg gcgttgtaac gatctccggc actgctgatc
     9781 tggaaagcta tctggctccg cagctgaagg acaagctggc tggcatgagc ggctatgaga
     9841 acgtaactct ggatacttcc atcctgaagg gcgtttccta caccgcaacc atcaccactg
     9901 atctggcaaa cagcaaggaa gtaaaggctg acctgagctt cacagccggc ggcaagacct
     9961 acactgtaga tactgctaca gattacatga acgctgtata tgacagcctg gaatcccagg
    10021 ttgaggctgc tgttgaccag aaggtcaagg atctggcaaa gcagtacggc atgacagaag
    10081 atgaagttcg tgcaaacgct gacttcgaca tcgctggcga cctggcagct ctgaaggctg
    10141 agaccgacaa gctgggcagc aagatcgaca ccgtgaagaa gaagtatgac gctgcaaaga
    10201 acctgtctac tgcaactaag acatacggca ctgctgacga ggcaatcgca gctggtgtta
    10261 actatgctgt aaagaagggc atccagtccg cagcaaacct gccgacaacc gttaatggtc
    10321 tggttggcaa gtacggcgca cagttcgaca aggctgtttc ttctgtaaac agcagcctgc
    10381 aggatgccgg cgtaaatgtt gagatcgctg taaagtccgg cgatgttgct gctctgctga
    10441 acggcgcgtc caatgtaact gttggcgcag ctgccggcac ttacaccgct actttcgaag
    10501 ttgctgatgc tgaatacgat gcagttgttg catatgtaaa ggctgaagtt gcaaagagcg
    10561 acaagctggc aaacaaggaa gttgaaagcg tagagaccac caagactgtt accgttaagg
    10621 gcgcagctgc tggcactgca tctttcaatg ttgagcgtga cgttgttgtt gttctgaagg
    10681 ataaggaaac cacaacttcc acttctgaaa caactgatac tacagagacc actgtaacaa
    10741 ctgcaactgg ctctgacaca gataccaaca cgcagactac tgtaacaact gtatctggct
    10801 ctgaaacaaa cactgagacg aatactgaga ccaacaccaa taccaataca gaaactaaca
    10861 ctgagaccaa cacagaaacc aatactgaaa ccaatactga aaccaatact gagaccaaca
    10921 cagaaaccaa taccgaaact ggaaccggca ctggcacagg tacaaccgag actctgccga
    10981 ctggcgtttc ttctgtagag gttaagctgg ttgacgctga gaccgctgag gacatctacc
    11041 tgtccaacga agagtccttc gacgttgctg gtttgatcga gtccgtaact ctgcacctgg
    11101 aagacggtac tgactatcag gtacaggatc cggcaactgc aattgacttt gcaatgactc
    11161 cggctgaggc ttatgcaact gttgaccaga aggctggcgc aggcaaggtt tacttcaacg
    11221 gcgaagttgg tctgaagtac aagggtgaag aagatctgac catcagcgaa accgttaagg
    11281 ttgctgtagc tctgaagggc gatacaaacc tggatggtaa ggtaacaagt actgacctgt
    11341 acgacaccat gctgtacatc gcactgaagg gtgctggtga gaaggaatac ttcgacaccg
    11401 taaagaatgg cactgcactg gagaccaagc tggcttacct ggcatctgat attgataccg
    11461 agtccaaggc tggcgcaaac acagacgatg ctaagctgga ttccaccgat atgctgtaca
    11521 ctcaggtata ccaggcatac aacggtgctg gtgaacacat cgactgggca gaggctctga
    11581 agatcgctaa cggcggtaag taagaatctc ttttcccgca actcttattt aagcttgtat
    11641 atgttggtgg tgtgaggtgc atatcacacc gctgatatat cacata
//
LOCUS       DAWENO010000056        11684 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2324_length_11684_cov_10.178691, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000056 DAWENO010000000
VERSION     DAWENO010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2324_length_11684_cov_10.178691"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1466..1475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9959..9968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11332..11341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2732 a         3290 c         2850 g         2782 t
ORIGIN      
        1 gtcaagagaa aatttgatac tttccaaaac ttttttcagc ggaatcctgt atctgccaaa
       61 atcgcatgaa attccgtcgg ttttgttgac ttttccgtac aaaacatggt ataatgagca
      121 aaagacaacg gaaggatgaa ccatatgaag aaaacacttg ctgctctgct gtcatgcctg
      181 ttcctggcgg cagcgctgcc gcagccggca ctgtatgccg cagaaacaga tgccccagag
      241 gattcccgga tcaccggaga tgtcaacggg gacggacaac tgaacgccgc agatattgtt
      301 ctgatgcaga agtggctgct gcaagtcccg gatgtcacac ttgctgacgg gcaggcaggc
      361 gactgcaatg cagacagcag actggatggt cttgaccttt gccggatgcg gcagctgctg
      421 accgcacctg caccgcagaa tccactggaa cagctggtgg gaatgtccta tgcagatgca
      481 gtgaaaaacg gctatatctc cagatcggaa tacaactatc agatctccgg caatctgaag
      541 agtaccattg aaacccaaat ggaacgtccc ctggactact ccattgaccg gttttatctg
      601 gtaagcaatg aaacgctggg gctgaccggt gacacaaaat acctgtacaa ttccagcaca
      661 gcggatgttt atcccatcac agaagagacg agcatgaact gcgccacctg gtactggaaa
      721 ggcaaaaagg cggcactcta cggtattgat gacgacgccg acacacagaa caagtttttg
      781 gatgccatgg agttctacga cgtcaccgag atctactact ccatcggtgc aaacaaactg
      841 gtaaacagcg tggatatggt ggaaaccttt gtcaagaacg cctacgccag aaatatgaag
      901 gtctacctgt tgaccggaga aaacacctgg ctgtatgagg acagctatca gaccgccatc
      961 tatcgggtat tcgaccgggt ggcagagtat aacagcatgg tggagtatga tgcccggctg
     1021 gctggcgtat cctacgatgt ggaggtctgg acaaattccg aatataactg gaaaaacaat
     1081 gacgccgcac ggtatcagca ggtaaagttc atcgaaactg cacagcagta cgccgaatcg
     1141 aaggacttgt ccgtctccta ctgcctgccg ttctggatcg tccgctatga ctacacagat
     1201 gatgccggcg aaacacataa cgtatacgac tctatcaccc agattgccaa cgagaccatt
     1261 ctcatggcat accgtgacag tgctgctgcc gtggaaaagc tggtggcaga ggtacagacc
     1321 ggtgcatccc ggtctgtcta cgactacaac gagaaaaacg actgcaacct ggaaatcgca
     1381 gtgcaggcgg acgagaacag cgagggcgac catgtcactt tctatgaaga ggaaaaggaa
     1441 catcccggct atctcaatac agaaannnnn nnnnngcgat tccgctgtat gagtactacc
     1501 tttctctgga atcataaatg cctgctctgt ctctgacaaa aaggaggatt tctcatgccc
     1561 aaccgctgca aatggtgcaa tccaaacaac ccgaagtaca tcgcctatca cgacagggaa
     1621 tggggtgttc tgcggacaga cgacgcctat ctgtttgaga tgctgctcct ggaatcattc
     1681 caggcagggc tttcctggga gtgtatccta aacaaacggg aaaatttccg aaaggcgttc
     1741 gatgatttcc aggcagaaaa aatcgccgcc tatacagcgg aaaagtgcag tgttttggct
     1801 gcggacaagg gcattgtgcg aaacaggcgc aagatccagg caagcgtccg gaatgcggcg
     1861 atcttccttg ccatttccgc cgaatttggc agtttttatg actatctgtg ccagttcaca
     1921 cagggcagga tcttttacga aaccggaaaa gccacaagtc ccctgtccga tgccatttcc
     1981 accgacctgc aaaagcgtgg catgaaattt gtcggcagta ccatcatcta tgcgtattta
     2041 caggcggttg gcatcctgta ttcccacgat gcaggctgcg atctgtacag gcattcttaa
     2101 aatgcaaaaa agcatacagt atccgttccc ggaaatactg tatgcttttt cattttccaa
     2161 aaattatccg gcaatgccac gaatcgcaga caccgtctgc tgataccggg ttttcactgc
     2221 ctggaaagcg tctgcgtagt ccaccgaaag gtctcgccca cgcagttcct cggtcagttc
     2281 cgagcttgcc tgccgcagcg gttcaaaccc cagattgagg caaatgcctt tcagcgtgtg
     2341 cgccatgcga aacgcctctt cggcatcgtg cgcatccagc gcatcacaaa gaccggaaaa
     2401 cgtcttgtcc tccaaaaagc gcagggcgaa cttcgtcacg atccgttcgc tgcccagccg
     2461 ctgtaacaca ggcgcataat ctgcacccat ctttgtataa cattcctgta ttgtcatatg
     2521 attccattcc ctcgatttca caacgcagcg tattcatagg cgttcttgct ctgttccacc
     2581 aattcccgtt tcagcgtgtc ctgtatctcc agaaggcact ccagcagaac cgggttaaac
     2641 gcaccgcatt ccccgttcag aatcatctga atggctctct catgggaaaa cgccttttta
     2701 tatacccgtt cgctcaccag tgcatcatat acgtctgcca gcgaaaccac ctgtgccgaa
     2761 atggggatct catcgcccac aagcccgtcc gggtatccct ttccgtcgaa tcgttcgtga
     2821 tgccaccggc atatgtcatg cgccacacga accagcggct cgctctggta gatcgccagg
     2881 ctctccagca tattggcgcc aattactgtg tgggttttca tgatctcaaa ctcttccttg
     2941 gtcagccgtc ccggcttgtt cagaatcttc tcgtcaatgc cgatcttgcc gatatcatgc
     3001 agcgcagccg ccagcggaat cagcatctga tctgcacttg tgatctcgta ctttgtgtct
     3061 ttccgttcca gctgatccag cagcagttct gcaaggcgct tgatgtggat gacatggctg
     3121 ccgctttctc cgttccggaa ctctacgatc tggctgagaa tgctgaccat catgctgttg
     3181 tttttgtctt tctcccgtac ctgttcgctt atcatggaga tcaaacgccg ctgtttggcg
     3241 tagagcttga tggtgttgaa cacacggcgg tagacaactc tcgcatcaaa cggacggctg
     3301 atatagtctg aaacgcccat ttcataagct tttttcacgg aggaggaaga atcctcgctg
     3361 gagatcataa tgaccggaat atcgtcgatc caatggtttt ctgccatatg atgcagcagt
     3421 tcaaagccgt ccatcacagg catgatgata tccagcagca ccagggaaat gcttgtcccc
     3481 tgccgctcca gcagatgcag tgcctcttcg ccgttgactg cctcatgaat gatatacgag
     3541 tctcccagca tttctgccag aatcgcacgg ttcatctccg aatcgtccac gatcagaatt
     3601 tcctgcttgc tttccttgtc cgcaacagaa tctgcctcag agataatcac tgcatttttc
     3661 ttgttcttcg cctggtacat caaaacatcc gcacggtgta ttgcctcatc cagcagggta
     3721 ttttcgcagt acacgccgcc gatgctgacc gaaaggtgga taccggaata ccccgggacc
     3781 ggagcattgt gtatcttatg gcgaatgtgg tgcagctttc ggtaaaactc ctgctcatcc
     3841 gcatttgccg acagcagtac aaactcatcg ccgccgtagc ggatcagcac atcattgctg
     3901 tcagagcttt cccgtatcac cttgaccacc gtttccagca ccatatcccc ggcatcgtga
     3961 ccgcaggtgt cattgtacac cttaaagtca tccaggtcga tcatggcagc gccgccggta
     4021 aacttcttct gctggatctg tcttccagat accgccggtt atacgctccg gtcagtgcat
     4081 cccggtacag ttccacatga tactgcgaca gctgccggat cagcttgtcc cgttcacttg
     4141 caccgatcag cgaatcgctg tccagtttcc ggatcagttc catcacacag ggctttccgt
     4201 ccacttcaac ataacgggaa aagacctggt agatatctgt ccccagaaat tccagtttcg
     4261 tgtgctgctg tctgtcccgc agtgtctgca tggaaatgca gttttcacaa ggctgtccct
     4321 tgccccaata gtcatagcat tcgcaaaaag acgcatcttc tgtactttcc cggatacctt
     4381 ccagatcttc gcccttcaaa aggcgcacca cggtaaacac attccggaaa aagcgcatct
     4441 gctcttttgc ctgctgtgaa gtcagctgat agtcggactg tgtctcatgg tttgtgctga
     4501 atgtttctaa gatttccact gctgtatctt cctttcagat cggatggaaa aggcaaaccg
     4561 caaagaagtg ccgctgcgaa ccccgtttca tttcttcaca taccctaaat cataagcaat
     4621 accgtgcaaa tccgacaggc agacttggtt cggttctgcc tatagtatac cataatttat
     4681 catcatttgt caatcattgt tttcttctgt catcaatatc cctgcacgcc ggatctgaaa
     4741 ccgttcccgg cgagtgagcg tctttcgctt tgggtcagat atctttttca gaaacgatgt
     4801 cagcacacgg acttcatagg gctgaagcgt cgtttccccg tagatgtact tttccatcaa
     4861 ctggttgaca cgctcgtatt ctccgtcaca cagttccggg attttttctt tcatccgctg
     4921 ttccgccgtt tccgcctgcc agccgatgtg catttccacg cccatgcaat gcagcatttt
     4981 cgggatcatc atgcagagaa tatcctgttt ttttcgctca tcggctttct gataccgatt
     5041 ctgagagcga acctgcgtta ctgtaatgcg cacttccagg acagcagttg ccgcagccgc
     5101 cagaatcagc agcagcgcag cgattcccaa cagtagcact gccgcatttt ccaccgtatc
     5161 caggacactt cctccggaag tatcccgttt gctcttgttc tgcacattgc cggtctgtgt
     5221 ctcgctgtca tcggcgatct tcaccttttc cacattctgc ggctgctgta ccatctgcat
     5281 cagggtatag acatcgtgat aataccccgg tgttgcatca atgggaatcc agcccatgcc
     5341 gtccatatag acttctgccc aggcgtgggc atcctttgcc gtgagctgtg cgctgacctt
     5401 gtcccgcacc agcgtaccat taacgtagta gccctcggca taccgtgccg gaatgccaaa
     5461 cgaacgatac gccagaactg ccgccgttgc ataaaaggca gagtttcccg ttccgccgtt
     5521 cagaaacttt tccagcggct cttcggaatc ggttcgcccc ggctccatct ggtaggacga
     5581 ggtgttttcc agaacgtcat ggatgtactg ggtcacctgg tagacggtgt tatccgtctc
     5641 cgtataggat ggatcatgaa acttttcctg tatcacagag cgcagttctt ccgggatatc
     5701 caggtaattc tggtacacga aactgcggta gatcgcctcg cagtccagat acgccttttc
     5761 tgcctttgtc tccgggtgca ttgcccagtc gtccacatag agcagctctc ccggataatc
     5821 gttggcgtat tccgtgaaat cataccgctt tgtaccgaac agcccggcag actgcaccgc
     5881 cgcatcccgg tgaactcggg tggaacagtc cggctgatct gccgaatagg gcaggtaaac
     5941 ataccgtcgg tctgcgccgt tgttttcgac ggagacacgg tttttcggga actgttcccc
     6001 accagcagtg ccgtattcgc agtactgctg agacggtgaa aactgatgtt ccacaaacca
     6061 ggactcgatg ccgtcatttt ctccccggaa gtccgaacgg ttcagttcct gccatttgcc
     6121 gttgccgtaa tccgcaccca caaatccacg caggtacagg tctttcgcct gagtctggcg
     6181 caccatgagc cgatctttat caccccggtt catccggtac gccttggaca gatctccttt
     6241 cggcagtgtg tcctcgccgt aacgcacccg tgtgatcgct gcacggacat tctcacgcag
     6301 cacattcacc gtgtggaatt cccggctgcc ggatgccgct gccgcactca gaatcaccgc
     6361 agacgctgtg acgaaccatc ccaccttttt ccagctgaaa gaacgctcac agtgacagat
     6421 gcgctgtaag agccagccga tcagatacag ccccacgccg atgccggaaa agcattgcag
     6481 gagcagtgcc ggcagcgtca gaaccagcag aacgaaaaag ctgacagttg gtttgttgat
     6541 ctgcaccagg agatatcccc aggcggcgat cagcagaatg gtaaagatca gagagaaaac
     6601 gccggctgtg cctgtgccgc ccaaaagcga gactgcatca tggctcaccg tgttccatct
     6661 ggagatcacg ctgttgagaa aaaggatcgc tccgtttttg cagtccggga atccgatcag
     6721 cagcaccagc agtgccagcc cgttcggaac cagcagtgct gttttgcgaa acggtttcca
     6781 gagggacagc gaatggcaca aaaccgccgc agctgcacac agcagtaccc agaaaagcgg
     6841 tacctctgcc gttccgtagt gcaggaatga cacacagaac ccggcagaaa acagcccggc
     6901 gcaaatacat tccaaaatca tattgtacac aggaggctgc tccgtaaaaa ctggcatggt
     6961 ctccggtttc ttttttggtt ttcgatgcag ctgcatctgg atacacctcc tcagcacaat
     7021 gtccggaaat ggattcttat agtataatgc ggaaactccg gtttttccgc agatatgtca
     7081 ccggcacttc gaccagcact ttcgcccttg ccggctgtgt cacaacaggt acgccatcct
     7141 tgtccgtgac gactacggca tccaggttca catccgccgg gatcagcgcc agttccttgg
     7201 gaaagctgtg tatggtaaag tacaccatgc tctcatagtt ctgctggatc tgccctgcca
     7261 ggaaaaactg acaggcgatg ccgctgttct ccgccagcgg ataggacagc agcgtgtaga
     7321 ggaactctgc ggcatctgcc tccgctgcaa tgggtgcgga aataatgcgg ctctgaaaga
     7381 caaacgccgc catatgcgga attccggcac gcaccagcgc acggctcaga ctttccactg
     7441 cgccgatgga tgtggaaagc aatgtcttgt ccacatcctc gccgtttgcc ttcagcgcaa
     7501 tgtcatacag cacgatggtg tcataccggg tggaatcgct gtattcccgc acgatcagcc
     7561 gatccatctt gctggtcagt ttccagtgga tggctttcag ctcatcgccc tcctgatact
     7621 cccgcagttc gaacacctct gcggtatcgt ttcccttttg gtagacccgg aacgagccga
     7681 cttccggctt tgcccgggat gcctggtcgg tcatgatggt gatgtcattc tgttccggca
     7741 tcaccttgac acggcagaac aactcgcagg gtactgcggc ggcacagaac ccaaagacgt
     7801 ccaggcactg cacaccagat gcccggataa accattcgcc gcacatatcc ggcgtaaact
     7861 ccacatgaga gcttttccgg gaagaaagac cgatccgcag ccagcgttcc tcctgtgtgt
     7921 gaaacaacag gttctgcatc tccagggtca tttccactgc agacaccgaa aacagcggac
     7981 gctttgcctt ataggtgata tccagcgcaa gcggcgcatt gacattgctt gtcaccggtg
     8041 cttgtaacga aagggtcatt gcagcaatat ccatccggag cagaacgagc gccagaagcg
     8101 gcagggcgat ctccaggagc agcactgcca gaagaatcgg tttcgggaag aaaaacaaca
     8161 gcaccagcac gactgcgatg gacaaaaagt agctcacctt gtgccggatc atgacagctt
     8221 tcctccgatg gtcaccggcg gtttcacctt ttccaggatt ccctgcaaaa tctgcacctt
     8281 gttcacgccc tcgatcagcg cctggggacg caggacaaga cgatgaacgc agacatccag
     8341 atacacgctc tgcacatcct ccggaactac atagttccgc tgttctacca gagcgtttgc
     8401 ctttgccatg cggatgagtg cgatcacgcc tctggggctg atgcccagtt ccacaaaggg
     8461 atgcacccga gtctcctcgc acaggcggat ggcatagcgc atgatatcct ctgtcacctt
     8521 gatggcagag agatagtcct gcacctgcat cagactcttg gcatcgcaga ccggcttgat
     8581 ctcatggagc ggattggtat agcgtctggc gttcagaatc ttgatctgat cctcacagga
     8641 cggatagccg atggaaagct gtatcatgaa acggtccagc tgggattccg gcagcggatg
     8701 cgtaccggat gcgcccagag gattctgcgt tgcgatgcag ataaacggac tgggcagcgg
     8761 atgggtcacg ccgtctacgg tcacgttctt ctcttccatg gactccaaaa gcgccgactg
     8821 ggttttcggt gaagtacggt tgatctcatc tgccagcagc agatggcaca ttaccgcacc
     8881 gctgtgatac tcgaactgat tgttcacccg gttgaacatg gaaaagccgg taatgtctga
     8941 cggcgtggta tctgtggtaa actggatacg cttaaagtcc agccccagcg cattggcaaa
     9001 cgccagcacc agcgtagtct ttcccacgcc cggacaatcc tccagcagaa tatgaccgcc
     9061 ggcataaatg gtcatcaaaa ctttccggat gatctcatcc tttcccataa atgccttttt
     9121 gacctctgta ataatatgtt ctgactgttc gatcaccatt gccgcatatt ctcctctgcc
     9181 tgttttagtt tttccaggac ttcctcccgg tccggttccg gtgctgtttc gctgcacccc
     9241 agcagatccc aaagcgtctg ccacttgggc aggaatgtat ctgtgatctc ctgtgcgatc
     9301 tcctcgatgg atgtaccgtc tccggctgac tctgcgtaat attcgctgca aagactttcc
     9361 aggtcggaaa aacctgcata gggatactgc cacgccgccg cataggtttc ccggcagccg
     9421 tccggcacag ccagcggcac tgtgttgaca ctgccgtctg acttggactg gaacacaaaa
     9481 tcactgccct gtccgtatcc gatcagattc ggcggtgttt ctccggcaaa gcgaatctct
     9541 tccagcgcat cacagccgcc gaaaatattg taatagacct cgtttgtggt ctccgggaac
     9601 gtaaagctgt gcaaagctgt ttctgcaaat gcaccgtcat gaatggattc cacgcagctg
     9661 tttgctccaa aggtcacctc agaaagcgct gtacacccgg caaacgcatt ggacggaatg
     9721 gaacgcagtg tagaaccagt ctcaaaatgc acggcagtca gattgctgca atcctgaaac
     9781 gcattgctgc ccagcagtga aaaggcatct gaaaatgtca gttctccgct gatacccgaa
     9841 ttttggaacg ccgcatcgtc cacaaacatg actgacgcaa agctttcagg tgcaatgaaa
     9901 aacgcattct gacaccggga gaatgcaccc gatgcaattt ccacggtgtt cggtgctann
     9961 nnnnnnnnta tatgttccgt tcaagttgcc ggaaacggac aacaggtaac tctcttcccc
    10021 ggaacttccg tagacaccgg caacaccgtc tgcgccggtc tccattcggt accgataggt
    10081 aaattccgca aagcagccgg cgtatccata ggaatcatac gggcggtaga aaaagctgtt
    10141 gttgccgggc gcatagtttc cctccatcac cgcagatgcc gcattgcagg caacgcagta
    10201 gagcatcatg cagttgtcga atgcgccgtc gccgatggtc agttcatcct cagacaagga
    10261 caccaccgat tccaaagcac tgcacccgga aaaagcgttc tgctgaattt ctttggtctc
    10321 ctccggcagt tctgcggtca tcagcccgga acaattccgg aacgcacggc tgccgatggt
    10381 tttgatggaa acgcccggca gactctccgc actgaaatgc acagtatccg gcgcagcggt
    10441 cagaagtgtt gcaccgtcct gttcctggaa atagacgttg ccgtccgttt cacaaatggc
    10501 atacgcctca gcggcgatgg gaatatcata ggtattccac tgttccgtat caaactccat
    10561 gctgaacgcc ggaatgtgca gaccgccgat attctcagaa aagctgtccg cctgcgcagc
    10621 cggagcagtc tcaccctgga agtagatctg cgtgccgtcc agcccgttga atgcaccgct
    10681 gtccagcgtt tgcagggatg caggcagaat cagcgctttc aagtctcttc cctcaaatgc
    10741 gcccttccga atctcggtca ctccctccgg cacggcataa gtaccggcta cctgttctgt
    10801 cagaccgtac agcacagtgc cgttttctgt atttagcaaa atggcgtcat cctgggtata
    10861 gaaatccgtg ggattcttgt cgctctgggg aacgagctgg tttgtgccaa gacgcattgc
    10921 ccactttttc agatagtcga agtaggcatc atccggcaca tagatttttg catgatccag
    10981 ccggcgtagt tcaatttccg gcggctctgc ggactgaaaa gttaggctct gaatctgatt
    11041 ttcttccgga aaagtcacct ttttcaccgt ctccggaacg gtgatctccg ttttcgcata
    11101 cggcactgca tacagttccg tgcaggcttt gttgtacaaa acgccgtccc gggacgtgag
    11161 cgccggattc ttttctgaga catcgtagga atcctttacc ttcagcgtgc tggtgatttc
    11221 cagaacggat gccggaacgg aaacacggtc tacgtctgcc tccagggcaa ccttttgcag
    11281 ccctgccgga atctccagat cacggctgga accggtatat ccggtcagtg tnnnnnnnnn
    11341 ntgtctgaat gaaatagtag tggtcatcct ccgaccacat ggaactgatc tccgccataa
    11401 actccttcgg gagttctgcc gttcccaaag gatacaggat attcaagcct tttttcgttg
    11461 gggtatagcg gtagtagacg gggtcgccgc cgttgttctc cgaccatccc aggaagatct
    11521 gacggatgga ttctccgagt ttagggaacg aatgatccga atgatacatt ttcccgtagt
    11581 aattcagcag atcgacagac tcgttttctg ccggatactg gtcactcttg ggattgatga
    11641 aatccgcatg gttataatcc gaaagcaccg tcttataggg atac
//
LOCUS       DAWENO010000057        11674 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2327_length_11674_cov_11.179275, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000057 DAWENO010000000
VERSION     DAWENO010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_11674_cov_11.179275"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2861 a         3150 c         3041 g         2622 t
ORIGIN      
        1 actcttcttc ctcctcttcc tcgtcatcat actccggctc gtccccgaag tcatcctcct
       61 cgaactcttc aaagaaaaat tcagacagca gttccttggc atcgtagttg ccacgttgtg
      121 ccgcctggtg cagccagtac tgtgccattt cgtcatcctg gtctacaccg gttccgttca
      181 tgtagcagta gcccagcata tactgcgctc cgtcatgtcc acgctcggca gccttttcga
      241 accagtccac agcgtcctcg tcatcctcgc tgtcgtcgcc tacataatag cacacaccga
      301 tcatatactg tgcgttttca ttatcctgtt ttgccgcctt gtaaaaccat tgcagggcgg
      361 tttctttgtc ctcttcctcg tcgaaatagt atgtaccgat ggcacactga gcatccgaaa
      421 gtcccagcgc cgccgctcgt tcaaaccagt ccagggctgt gtcgatatcc tcatcgacac
      481 cctcaccgtc ctggtagcaa gtgccaaggg caaactgcgc ataggcgttg ttctgctttg
      541 ccgatttttc cagccaatat accgcctctt cggaatccgg cttgacgccg ctgccgaacc
      601 ggtagcacgt acccagctga aactgtgcct ctgcatctcc ggcatctgcc gcctcccgga
      661 acagccgcag cgcatacttc gtatcacgct tgacgccgtt accgtccagg taacagatgc
      721 ccagctggca tttcgacggg atgtcgccct gttctgcggc tttctgaaac cagtagaccg
      781 cctggctgtc ctctgtctct gaggtcactt cctggtagta caggcacatt cccagggcat
      841 actgggcatc ccggtaatcc tgctttgccg ccttttccag ccactcctgc gccagctttt
      901 catcctgggg cagtcccctg ccgaagtgat aacaagtccc cagcagatac tgcgcaatag
      961 gaacgccctg ctccgcagat ttctgatacc attccaccgc ttttttctcg tccagttccg
     1021 tgccgaggcc attgaaataa cagtcaccca gattgtatgc cgcagcggcg tgtccgtctg
     1081 ccgccgcctt ttgaaacagc tcgaatgcct ttttctgatc ctgtgccaca ccggtaccgt
     1141 ccatataaca gcgccccagc atattgactg cctccgcatt gccggactgc gccgcctttt
     1201 caaagacgga aaacgccttt tccgcatcct ccggcacgcc gtcgccggac agatagcaaa
     1261 gtcccagctg gagcatggct tcttcattgc cctgcgctgc actgcggctc agcaactgta
     1321 ccgcctgttc cgcatccttt ttcatgcgct ctccgttcag caggagcatt cccacggcaa
     1381 actgtgcctc cgcagagccg gccgctgcgc ccttttccag gtaatagcga gcctttgtga
     1441 aatcctctgc cacgccatgc ccctggaaat agcagtcgcc cagatggaac agtccggacg
     1501 gcaattcctg ttccgccgcc ttttccagcc actggatgcc tgcggtttcg tctgtctctg
     1561 ttcccgttcc gtaaatgcag cacattccgg tgaagtcctg tgcgtcagcc agtcctgcct
     1621 ccgccgcacg gctcacccag gaaaagccct cttcccggtt tgcctctgtt cccacgccaa
     1681 agtacaggca gcgtcccaat tcatactccg cctttgccac gccgctcagc gccgccgcct
     1741 ggagcagcag aaagccacgc ttttccgcat ccgtaccctc tcccggcaga ttcagcaggt
     1801 gcattcccag aaagtattcc gcatcatggt cgtgctgttc cactgccagt tccagcagcc
     1861 ggagaccttc tgcctcatcc tcttccaccc cgtccatgcc gccgtgcaga tgatacacgc
     1921 ccagcaaaaa ctgcgcctcg tgatagccct gcgccgcaga gcgttccagc cattccagcg
     1981 cctgttcacc atccggttct gttccgatgc cgtcccgcag gcacatggct gtgataaact
     2041 gcgcattggc atagccctgt tccgccatca tccgaaacca ggcatacccg gattcctgtg
     2101 cagacgcatc tccttcttcc acctgctcca ggtgacggtt atagttctcc ggattcatga
     2161 tgttcgccgg atctgttttc cggaatcgtc ccaaaaattg ctttttcatc aaaaatcccc
     2221 ctttctcatt ctgctgtttt tgtcgtgata cttattataa agcaaaatgc gcctggtgtc
     2281 aagtaaacgc cggtttttgg cgcaaaaatc aacgggaaag ttccccttaa aaaacggttt
     2341 gcatgggttt ctacaaggtg cattatctct ttcatcagat ttatatgtta actttttgta
     2401 actagttgag aatcagtctt gacaatcaat ttttcctgtg ctataataag gacaagcaaa
     2461 gggagattgg atcccatgca aaaattactt tatggaggta gtactcatga aaaagtttgt
     2521 atgttcagtt tgtggttatg tatacgaggg cgaggctgca ccggcagcat gtcctctctg
     2581 cggtgcgccg gcagacaagt tcgtggagca gaaagaagag aatctggcat gggctgacca
     2641 gcacgttgtc ggcgtagcac agggcgcaga cgaagagatt atccagggtc tgcgtgagaa
     2701 cttcaacggc gaatgctccg aggtcggtat gtacctggca atggcacgtg ttgcgttccg
     2761 tgagggctac ccggagatcg gtctgtactg ggaaaaggct gcttacgaag aggctgaaca
     2821 cgctgcaaag tttgcagaga tgctgggcga agttgttacc gactccacca agaagaatct
     2881 ggaaatgcgt gttgccgctg aaaacggtgc ctgcgcaggc aagaaggctc tggcagcaaa
     2941 ggcaaagcag ctgaacctgg atgccatcca cgacacggta cacgaaatgg caaaggacga
     3001 ggcaagacac ggctgtgcat ttgccggttt cctgaagcgt tacttcaacg actaagtttg
     3061 tgcggtgttg cctataaatg aaaagcggac tgtttcggaa gaggttctga aacagtccgt
     3121 tttttgctta taatgtaaaa atttccagat tctacccgca aatatccgcc cattccgtcg
     3181 gaatcccaac gctgttccgg gcgatataca gcatggtata aaacacatca gtggaatcca
     3241 ctataccatt gccgtccaca tctgccgcct gttcctgggc agccgtccag ccggattcca
     3301 gtcccacgcc acgctgtgca attcccagca tgagataaaa cacatccgta gaatccacct
     3361 ttccgctgtt gtccatgtcc cccaggttca gcatcgccgg ggtatcgtag tgaatcactg
     3421 caccgtccag cccgtcattg tcgtcgccga tgggcagcgc attccattcc gactcagaca
     3481 agacagtata aacgtcagtt agctgcgtgc ttagcgaaaa ggcactctga ctaatttccg
     3541 tcagctgtcc cttgagcgtg accttttcca aactgagaca attggcaagc acatacgccg
     3601 gaatagtggt gacaccggcg ggaaatgtga tctcacgcag cgccgtgcag tcataaaatg
     3661 cccttttgtc gattcttgtc acactgtccg gaatctccac cttttccagg ctgtggcact
     3721 gccagaatgc cgatgcgccg atggtttgca ggctttccgg cagaatgacc tctttgatct
     3781 tcagattcac cataaatgcc gcatcacaga tctctgtaac gccctccgga atcaccagcg
     3841 tctcatccgt acatttttca ccgtctatca caatatggtt gacctgcacc agcggatctt
     3901 ccgcctggcg ctcctccagc cagggcgttt ggaaaaacac gctgttcccg atattccgga
     3961 cagttcccgg aatcgtgatc tctgacaggc tggcacacga catgaaacag ccgctgccga
     4021 tggtaagcag atacttgggt agctgcacat gattcagctt ggaacagcca tagaacgcac
     4081 gtgatcccag cgcacgcacc gtatccggga tcgtgacatc ggtcagttct gtgcggctgt
     4141 aaaatgcgtt ttctgcgatt tgcagcaccg gcttgccttc gatctcttcg gggatcgtca
     4201 cgctgacggc gctcttctca caggaggtaa taatggcacg gtcagaggtg agggtgtatt
     4261 tcagttctcc gtaagtgctg gtgttttctg ccgcagcggc acagatctcc ggcacagccg
     4321 aaattgccgc cgggctgaca gaaagcgcca gcattcctgc tgtcaggaat gtgcaaagtt
     4381 ttttccaaag ctgcatgata tccaaattcc tttccatgca caaaaaggca atgccgcatc
     4441 cgtcctgcaa tgcagcattg cctctgttgt atcattttca agtcagtatt tccgaggaaa
     4501 cgctccccgg ttcagaaact tctgagcgtt gtggtcagaa acaggctgtt gtccatgtgc
     4561 atcttattca gcacatatag tatgatcgcc atgactgtcc agatcgcagc cgtttgaatg
     4621 gtataaaata taatcttctt catcaaagat acacctcatt tcacaaagta ccgcccatgc
     4681 cgttacggca ctttggtttc tacatctatc attatacaca ataactgcca agaatacaat
     4741 agctttttgt gaattgtaat taaattgcaa ccgttttttt cgaaatgaca gaatccggaa
     4801 caaaagactg cgccggtatt cggtataatg cacagtgctt tttttcgagt ctgttgcaga
     4861 accataaata ccaccaaaaa accgccaact tttccgaaaa acacacgggt ttctcgtgta
     4921 atattccata tttacaaacg gaacagtttg tggtatgatg aatatgatga gcaatatcat
     4981 gatattatga aaaggaaagg ggctttctcc atgcaaaatt atgtaatcac cattacacgg
     5041 cagttcggca gcagaggccg ggacattggc gttgccgcag cgaaaaagct gggcattccc
     5101 ctatacgaca gaaatgtgct ggaaaaagag gcagaggaga tcgacagcac actccattct
     5161 ctggtggaat tcagcaaaaa cggcatgagc gaatcgggat attacaagat ggcatatcct
     5221 ctgggcatcg gcagtgcgct gaaaaaagac cggatgtttg aattgcagag caaattagtg
     5281 ctgaaacacg cagaaagtga aaactgcgtt atcatcggac gctgtgcgga ctatctgctc
     5341 cgggagcggg aaaatgtgat tcgctgctat atctttgcac cctatcaggc acggatccgc
     5401 aacagcatca tgacgctggg actcgccaac gccgagccgc agcgcatcat cgaagaagtg
     5461 gacaaggcac gtgcagagta ttaccgctcg cacaccggct gtgaggtcaa caatacccgg
     5521 taccgtgatc tgtttctgaa cagcaacctg ctgggcgtag aaggcacggc agatcttatc
     5581 gttgacgctg tccggagaaa gttcggactg ccggaagaaa aataagaccc gggctgtaca
     5641 ggcacaagtc ctggggattc cgcatatcct gtcactgagg tgatgcgtta tgaaggaatt
     5701 ccgcagaagc gtctggatcg gttttatctg gagcatcgtt ttcggaacga tgctccattt
     5761 tttatacggc tggacgggcg aaaaccgggt ggtagggctg tatgccgccg tcagtgaaag
     5821 cacctgggaa catctgaaac tcctattttt cccggtgctg ctctatacgg tctgggaata
     5881 tatctggctg ggacaccgct ggaaaggcta ggtacgggca gagagtgtgc tgctgggaat
     5941 gctgaccatt acggcgctgt tctataccta caccggcatc tgcggcaggc actggctgtg
     6001 ggcagacatc ctcacatttg tgctgggtgc ggctgtcact gcgtattaca cctggaaata
     6061 cgcccggcga ggacgtggca gcagcgtgtt cggcgcagtt gtccttgcag tcatcgccgt
     6121 ctgctttggc gtgttcagcc tgtttccgcc tgcggtggga atttttgcaa tcccataagt
     6181 atccgggcag tccttttaag ggactgccct ttttctgcac catttttccg gttctcacat
     6241 aaaatgtgac aggaggtgaa cgctgtgaac ggactgattt gtctgctggt attctgtctg
     6301 ctggcggtga cggctctggc agcactgggg ctgtgccggc ggctgttttc ccatgaactg
     6361 ccggaacata tggcgactct tgcactcctg gaaagccagg cgcaatggga acggcagtgc
     6421 gccgcactgg ctgcccagct gacctggacc gaccgggaca tggtgcacac agtctggctg
     6481 gtggatgcca cgccggacgg cagcctggaa cagggctgca cggcattttg cagacggcac
     6541 agaggctttt gctattgccg gctgtcggag ttgacgaaaa tttttggcag cggtgcggaa
     6601 tccgaaaaaa atgattgcaa tttgtcgaaa aaacaggtat aataaaaaga gtgcattggg
     6661 aaaggaggcg gtcaaagtgg cggcagaaga aagaaaacca ctccatttag aaggtactgt
     6721 ggaacacgtg atgtactgca acgcagaaaa cggatataca gtcctggaac tggatgccgg
     6781 cggcacactg attacggtgg tcggcgaaat gggcgagacc gaagagggcg aacagctttc
     6841 ggtggacggc gaatacacca cccatccacg ctatggaatg cagttccggg cgcagtactg
     6901 ggagcgcaag ctgccggcag atgcgctgaa catccagcgc tatctctcct ccggcgccat
     6961 caagggcatc ggaccgtctc tggcaaagaa aatcgtggaa aacttcggag atcacaccct
     7021 ggagatcatg gaaaaagagc cgacccgtct cctggagatc cggggaatct ctccgaaaaa
     7081 atgtgagtct atcgccgcag aagtcaagca gattttttcg ctccggacac tgatgctgtt
     7141 tctggcgcag tatgatatcc gctccaaata tgccatgcgt gcatatcaga aatggggcaa
     7201 cggcgccatt gatctgctgg cggcgaatcc ttatctgctc tgtgttcccg gcgtggaact
     7261 ggactttctg aaagcagatg ccgtggcaca ggggatgcag ttcgcacaga atgcgcccca
     7321 gcgtgtccag gcaggcatca tctacatctt acaatacaac accaacaacg ggaactcctg
     7381 tctgccgctg gatcggctgc gcccgaaagc gtgcgcctat ctgaaagtca acgaggcaga
     7441 tttcgagatt gcctatgccg gggcgctgga cgagcatacc ctctgtgtct atgagaaaaa
     7501 cggacgggac tatgtctatc tggaggacta ctacattgcg gaacactaca tcgcagaccg
     7561 catcggtgta atacaggatt tcagcgcacc ggaggacaac agcgagtttg aaaagatgat
     7621 cgacgcccag gaacaggaac agggcatgga atacgccgcc ttacagcgaa aagccattgt
     7681 gacggcgctt tcccgtggca ttatgatcct caccggcggt ccgggtacgg gaaagaccac
     7741 gacgctgaac ggcatcattt ccctgtatga aaagcagggc tatcgtgtca tgatcgccgc
     7801 acccaccgga cgtgccgcaa gccgcatctc tgacctcacc ggctatgagg caagcaccat
     7861 tcaccggatg ctggaagtgg aatacgacgc atccggcggc atgggattcc agcacgatga
     7921 gcgcaatccc ctggagtgcg atgtgatggt agtggacgag atgtccatgg tggacgtgct
     7981 tttgttcgag catctgctcc gggcgctgcg gctgagctgc aaggtggtgc tggtgggcga
     8041 ctgtgaccag ttgccgtccg tgggtgcagg caacctgctc cgggatctga tcgacagcgg
     8101 cagagtgccg gtggtggcac tgaaagagat cttccgccag gcgcagaaga gcagcatcat
     8161 caccaatgcc cacaagatca tcggcggtga atacccggat ctcacccaga aaaagagcga
     8221 ctgtttcttt ttccagcggc tgaagccgga tgctgcaaca gagctgatgc tggaactggt
     8281 caagacccgg ctgcccaagg catacggcta ctcccccatg gaggatatcc aggtcatcac
     8341 cccgtcccgc aagggcgtta tgggcgtgat cgaactgaac cagcggctcc aggcggtgct
     8401 gaatccgccc tcgccttctc tgccggaggt aaagtccatg ctctacacct tccgggaagg
     8461 cgacaaggtc atgcagatca agaacaacta tgacattatc tggcacaagg acggcgaaaa
     8521 cggcacaggc atcttcaacg gagacattgg ctatctccgt gccatcaacc ggcagaccca
     8581 ggaagtggtg gcggaatttg acggcagacg tgccacctat ccctttgaac agctggatca
     8641 gctggagctg gcatacgctg tcactgtcca caagagccag ggcagcgaat tccaggcagt
     8701 ggtcatgccg ctgctgggcg gattcccgaa actgtattac cggaatctgc tgtataccgc
     8761 cgtcaccaga gccagacggc tactgattat gatcggctca caaaaagtca tttatcagat
     8821 ggtggaaaac aaccgccgta tgaatcgata tacctgtctg cgggatatgc tggaacagca
     8881 aaaaagcact gcgcagacac agcgcctgga acagccggaa gcgtccggcg caaccgaaca
     8941 ggacgcacca gaacaggagg aatcgtcatg agtaaaacag acattggcat tgatttagga
     9001 acggcaaaca ccgtcatcac cttagggaaa aagggcgttg tactcagcga accctctgcg
     9061 atcgcctatc acacccacac caaggaagtc ctggctgtgg gcaagcgtgc ctatcagatg
     9121 atcggcaaga ccccggacta tattgaagtg atccgtcccc tttccggcgg cgtgatctcc
     9181 gacgaccgga tgacacagta catgatccgg gagtttattc tccaggtcac cggacatcgg
     9241 ctcaccaagc cacgcatcat tatctgtgtg ccgtccttta tcacggacgt ggaaaagcgt
     9301 gctgtagtcg aggcagccat gagcgccggc tcccgaaagg cgtatctcat tgatgagcct
     9361 attgccgctc tgattggcgc aggggtaaac atcggcaagg cacggggcaa catggtagta
     9421 gacatcggcg gcggcacaac agacgttgcc atcgtctcca tgaacggcat cgtcacctcc
     9481 cactccgtcc gtgccgccgg aaacaccctg gaccaggcga tcatccggta tatgcagacc
     9541 aagtacaagc tgctcatcgg cgaacgcact gccgagcggc tgaaaatcga aatgaccaac
     9601 ctctacgacc ccagagcgga tgttacggca tgggtcaagg gacggaatct ggtcagcggt
     9661 ctgccggaaa tgcaggaagt cagcgagctg gagatcttcg aggctgtgga agatgtggtg
     9721 gcggacatca tcgaggcaat caagaccgtc ctggaagaga caccgccgga actggtaggc
     9781 gacatctacg aaaacggcgt cctcatggca ggcggcggtg cgatgctggg cggtctgcga
     9841 aagctggtag agcggacgct ccaggtcaag tgtgttgtgg caaaggatgc cattcactgt
     9901 gtggcaaagg ggactgccat tgcgttccgc aaaatggaca cgctgctgga cggctttgag
     9961 aatattgcgg tgtatcagtt taagtgaggg tatgccgcca gccggcttgc tccctttcat
    10021 gatcccctgt ctgcaaacat gaaatagaaa cgggtacaca acgctgtacc cgttttttct
    10081 gccgttccat cagcttagca ctctgcatta gcacttgtta gcactcattt actaagagtg
    10141 ctaattttca tccagttttc acaaaaccga ccttgaactt tcacatagca ggggtaatat
    10201 gatacttgta aacggaaagg ggttcggaag atgagaaaaa gagagcgaac cgcatccgat
    10261 ccgttaccac aaaaataata gaacatcatt cgtaggagga atgctttatg atgacatatt
    10321 taacaccgtt tgaacgtaca ggttatgatc tgttccgctc catgcgagat tgggagaatg
    10381 aattcttcgg cggaaaatcc gctgcgatga gttcctgcaa gaccgacatc cgggacaccg
    10441 gtgacagcta cgtgctggag gcagagctgc cgggattcca gaaggatgag atcaagatcg
    10501 gcgtagagag cgacaccttg acgctgtccg ctgtccacaa ggaaaagacc gaagaggaaa
    10561 aggcaaagga cggcacctat ctccgccggg agcgcagcac cagttcttat gaacagaaac
    10621 tggacatcag caatgtggat gcgtccggac tgcacgctgt gtatgaaaac ggtgtgctga
    10681 cgctgaccat gccgaaaaag cagccggcaa agccggtttc ccgtcaactg gagatccact
    10741 aaagtaatgt tgaaaagaga aaagcaatgc agaaaaaaat ctgagatagc aaaaccaaaa
    10801 agaaaaagag agaaaaagcg ctgaaaaggc gcatagaaca aagaaaaacg agcgtgtaaa
    10861 gcaagcgctc agagacagtt ccaaggagga atgacttatg atgaccattt ttcaaccttt
    10921 tgcaaacaca ttgtggaata cagtgaaaga tactgagatc acaaaggtgc cgaaaactga
    10981 cctgcgggat actggcgaca gttatgtgct ggaggcagat atgccggggt tcaagaaaga
    11041 agaaatcaag ctgtccttgg agggcgatgt gctgaccatt gcggcagagc ataccacaga
    11101 aaaaacagca gaagatgcgt ccgatagtga aggttctgct gaacagactg acacggctaa
    11161 gaaaggcaca tacttgcagc ggcagcgcag tttctgtaag tatttgcagc gcctggatat
    11221 cagcaacgtg gatgcagacc gcattgatgc ggcatatgaa aacggcgtgc tgaaactgac
    11281 gctgccgaag aaacagccgg caaagccgga agtaaagcag atcaccgtgc agtgatcccg
    11341 aatgttctcc cctgcggtaa tcaccgcagt ttcataaatg tatctcctat atcataaaaa
    11401 gaggaagtct ccgaaagggg acttcctctt ttttcatgca gcgtctcttt tatgttgcgc
    11461 atcgaaagcc tcccactccc gaaatgtatc ccacaaaatg agatgcacgt ctttccggcg
    11521 gcatttactg ggaacacact ctgattcatt ttcttcatta taccacaatt ttcaacaggt
    11581 ttcaatagca gaaaatgtgc cgcaacgaat atcaattttc atgatagcct cccctgaaag
    11641 gggaggggga ccgccgttag gcggtggaga ggtt
//
LOCUS       DAWENO010000058        11315 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2425_length_11315_cov_11.126288, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000058 DAWENO010000000
VERSION     DAWENO010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2425_length_11315_cov_11.126288"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    561..570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2270 a         3292 c         3103 g         2640 t
ORIGIN      
        1 cactaccggc agtccttttt ccgtcgccgt ttcaaaggca cgggtgatct tttcgcccac
       61 cacgctgccc atggacgcca tcatgaaatg ggaatccatc acaataatga cggtgcggga
      121 acccttgatg gtgcattctc cggtgatgac cgcatctttc atgccggtca tggtctggag
      181 cgattccacc tttttggcat agcccggaaa gcccagcgga tcggcgctgt gcatttctgc
      241 atcgtattcc acaaacgaac cgctgtctgc ggtgagacgc atccgctcag ctgcggtgag
      301 acgtgcatga tagttgcagt gcgggcagac cttgttcagc tcctggaatt tgctttccgg
      361 cgtggtctgc tggcagcggg gacacttgaa caccggttcg tgttcctccg gtgcatcgtc
      421 gttgaaacgc ttggtgacgt tcttgaacaa ttcttccaat gccatagtta cagtttcacc
      481 ttactttccg ttgtccttgt cattcttgga atccgcaagg aatttctccg ccttgtgcat
      541 ttcttcttcc gtaaaggggc nnnnnnnnnn tctccaacaa agtgttgtca tatgtaccgg
      601 cctcgaaatc cttgttctta atcagccgca gctggaaatc gatgttggag tcaatgccgt
      661 ccacgataaa ttccgccagc gcccagcgca ttttcatgat cgcctggctc cgggtgggag
      721 cgtggacgat cagctttgcg atcatggaat cataatacgg cggaatggta tagccctggt
      781 atacggcact gtccacccgg atgcccggac cgccggggat atggagcgaa cggatcttgc
      841 ccggagacgg acgaaagccc tgttccggat tctccgcatt gatgcgacac tcgatggcat
      901 gaccacgcac ctcgatgtcg tcctgggtga acggcagggg cttgccctgt gccaccagca
      961 gctgggtctg caccagatcc acgccggtga cccactcggt gaccgggtgt tccacctgga
     1021 tacgggtgtt catctccata aagtagaagt tccggtcatc atccaccagg aactcgatgg
     1081 ttccggcgtt ataatagccg cacgcctttg ccgcacggac agccgcctcc tgcatggctt
     1141 tccgcagttc cggcgtcatc agcggactgg ggctttcctc cagcaccttc tggttgcggc
     1201 gctgcatact gcagtcacgc tcacccagcg ccacggtgtt gcccatctgg tcggcgatga
     1261 tctggatttc cacgtgatgc ggtcctacga tcagcttttc cagatagact tcatcattgc
     1321 cgaagaacag ctttgcctct tcctttgcca cagacatctg ttcttccagc tgatcctccc
     1381 ggtcgatccg acggatacca cgtccgccgc cgccggcaga tgcttttacc aataccggat
     1441 agccgatctc tgcggcgatc ttcttcgcct cttccacaga ggtcacagcg ccgtcagagc
     1501 cggggatgac cggaacgccg gctgccttca tggtctcctt ggcacgtgcc ttgtcgccca
     1561 gcatggagat agacgccgga gacggaccga taaaggtgat gccgttgtcg atgcacagct
     1621 gcgcaaactc tgcgctctcg gacaaaaagc cgaatccggg atgcagcgcc tctgcgccgg
     1681 tgttctggca ggcagagatc agcgcattca tgttcagata gctgtcctgt accgacggcg
     1741 gtccgatgca gaccgactcg tcggcgatct ctgcgtgcag cgccgtttta tccgcagtgg
     1801 aatagaccgt cacgcactgc agtcctgcct cacggcaggc acggatgatg cgcacggcaa
     1861 tttcaccacg atttgcaatt aaaacctttt tccacatatc gctgccgcct tactgctcca
     1921 ctgcgaattt gatctcacag gtcacggcac gcttgccctc tacgctggca atcgccttgc
     1981 ccacgcccac cggaccacgc tgtgcgatga tctctacttc cagatccagc acatcgcccg
     2041 gcaccacctg gcggcggaac ttcgccttgt cgattccggc gaacagagcg atcttgccct
     2101 tgaattccgg ctgggacaga atgcagactg cgcctgcctg tgccagcatc tcgatcatca
     2161 gaacgcccgg cgttaccggc ttttccggga agtgtccctt gaaccaaaag tcgtctgcct
     2221 tgatatattt ccgtgccaca acacgcttgc ccacttccat ttccagaacc tcatcaatga
     2281 ggaggaacgg gtcacggtgg ggaatgatct ctttgatctg ttcctgattc aacaatactt
     2341 ctgccatgtc tgcactcctt tacgcaagaa tcatcagcgg ctggtcatat tctaccgcat
     2401 cgccgttttt cacgagaatt tcctgtacta cgccttccac atcgctggtg acttcgttca
     2461 tcagcttcat ggactcgata atcagcacca catcgccctt tttcacggta tcgcccacct
     2521 gtacaaacgg cggctgatcc ggagacggcg cagcgtaaaa tgtgccgacc agcggtgctt
     2581 tgatggcttt gcctgccggg gctttcgggg ctgccggggt ctctgccgga gcagccggcg
     2641 ctgctgccgg aaccgcaggc atcaccggag cgcccatcgg cggcggacac tttttgccct
     2701 ttacggtaat ggttttttcg ccgtccgcaa tggtgatctc gcccagatcc tttgcggata
     2761 cgatatccgc cagcttttca atggtttcca gatccaccat gttataaatc ttttcactca
     2821 tgtctgattc tccttagtcc acacacttct tgaagcacag tgtggcatta tgaccgccaa
     2881 atcccagaga gttggacagt gcggcattta ccggcatttc taccggaccg tctacgcagt
     2941 aatccaggtc gcactccggg tccggggtgg tatagccgat agtctggtgg ataaagccgt
     3001 cacggatgga cagggcgcag acagccgcct ccactgcacc agtgccgccc agcatatgac
     3061 cggtcatgga cttggtggag ttgatcttga ccttccgtgc ctgctcttcg cccagggcga
     3121 tcttcactgc cttggtctcc aacatatcgt tcagatgtgt ggaagtgccg tgagcgttga
     3181 tatacgtgat atcctccggc ttcagtccag cctcggtgta tgcctcgatc attgccttgg
     3241 aggatgcgcc gcactcgtct gccggcgctg tcatatggta tgcgtcacag gttgcgccgt
     3301 agccagccag ctcgcagtag atctttgcgc cacgtgcctt tgcgtgttcg tattcttcca
     3361 gcaccagcac agcagagcct tcgcccagga caaagccgcc acggttcaga tcgaacggag
     3421 tggatgcctt tgccgggtcg gtctcggtgg tcagtgcctt catggtgcgg aaaccgccca
     3481 gggtgaattc ggtgatggtg gattcgctgc cgccgcacag gcagacatcc agatagccgt
     3541 ccttgatctt gcggaacgcc tcgccgatgg cgtgggtcgc agatgcgcag gcggtggtgg
     3601 tgcagaagtt gtcgccacgg aatccggtgc ggatggcaac ctgaccagcc gccatgttgc
     3661 cgatcatcat ggggacaaag aacggagaga tgcgtcccgg gccttttgca cggtacttgt
     3721 catgctcttc ctcgattctg ccaatgccgc cgatgccgca gccgatgatc gtaccgacac
     3781 ggtacggatc catatcctta aagtctgtgc cggcatcctg cagtgcctgg atggcagatg
     3841 ccacagcgat ctgcgtgaaa cgctccatgt ggcggatgtc cttgcggtca aagtattctg
     3901 ccgggtcgaa gtcacggata atgccggcta cgctgacacc cagttcggtt gtgtcaaagc
     3961 tgtcgatctt ggaaacaccc agtgttccct tctttatatt ttcccagaac ttttctacgg
     4021 tgctgcccag cgggtttacc gtacccagtc cggtgattac tactcttttc atatttcctc
     4081 tctctctgcc gccgtactgc ggcaaactgg gttcctagtg ctgccctaaa ttacatcatc
     4141 agtccgccgg agatctcgat ggtctgaccg gtgacatagc ttgcgtcatc cgagcagagg
     4201 aaggagatca cgtttgcaac ctcttccggc tcgccgtagc gcttcattgc caccagctcc
     4261 agcatcttgt tgcgctgttc ttcggtgagg gcgtcggtca tgggtgtctt gataaagccc
     4321 ggtgccactg cgtttacggt gacgtttctt gcgcccagtt cctttgctac agtcttggtc
     4381 atgccgatga tggcagcctt ggatgcggaa tagttgatct gtcccggatt gccgtacaga
     4441 ccagctacag atgccaggct gacgattctg ccgtggcgct ggcgcatcat cacagtaccc
     4501 accagtttca tcatgttgaa cacgctcttc tggttgactg cgataacggc gtcgtactgc
     4561 tcttcggaca tccgtgccat caagccgtcc ttggtgatgc cggcgttgtt gaccagtgcg
     4621 tcgatcgagc cgaaacgctc cttgactgcc ttggtcattg cctcacactg ggcatagtct
     4681 gccacgtttg cagcgaaaag ctctgcctct acgccaaagc tgcggcattc ttccgctacc
     4741 ttttctgcgt ttgctttatc ggaatcgctg gggaaatggt tgatggctac gttatagccg
     4801 tcctttgcca gacgctttgc gatgcaagcg ccgattccct gggatgcacc cgtaatcaat
     4861 gctgttgcca taatggtctc tcctttatct ttcaaatgat atcttcttat tctttgccca
     4921 gcagcttgta tgcctgtgcc cacatctcgt caatgacttc cttgcagggc ttgacatcgt
     4981 ggatcatgcc ggcgatgaga ccgcacagga acgagccttc gtccacgtta ccgtctacca
     5041 ctgccttgcg gagcgagccg acggtcattg cctcgaaatc ttccggcggg caggctgcct
     5101 tttccattgc ctggagcttt ttggtgtact tggtcttgat gccacggcag gggctgccgg
     5161 aatagaaacc ggtgatctgg ttgtcggtat cctttgcctt cagcaccagt tccttatact
     5221 tcggagagat ctggcactct tcggtcgcca ggaaacgggt tcccagctgt acgccctctg
     5281 cgcccagcat gaacgctgcg gcgatgcctc tgccgtcagc gataccgccg gctgcaatga
     5341 ccgggatgtc tactgcatcc accacctgcg gcacgagaca catggtggtg ttcatgccga
     5401 tatggccgcc ggactctgtt ccctcagcca ccagggcatc tgcgccggag cgctccatgc
     5461 gctttgccag tgccacagac ggcactaccg ggatcacctt gatgccggca tttttccatg
     5521 cctccatata ctttccggga ttgcctgcgc cggtggtcac gatcggcacc ttttcgtcaa
     5581 tgaccagctg tgccagatca tcggcgttgg gactcatcag catgatgttc acgccgaacg
     5641 ggtttttggt cttttcttta caggtgcgga tctggtcacg cagatagtcg atgggtgcac
     5701 tgccgcctgc gatcagaccg actgcgccgg cgttggagac agcagatgcc agtgtgcttt
     5761 ctgcgatcca tgccataccg ccctggatca tggggtattt gcaccccagc agttttgtga
     5821 ttctggtttc catagctatt ttccttccta actaatatgt attctttcga tcaatattcc
     5881 atgatgagcg ccgcataagt cagacctgcg ccgaaaccga ccaggcagac cttttcgccg
     5941 cgctggatct ttccggcacg gattgcctca tcgaaagcga tggggatcga ggcactggaa
     6001 gtattgccgt gatattcaat gttggtgatg accttttcca taggctgctt gaggaacttc
     6061 atcgcagtct caatgatgcg catattcgcc tggtgcggca cgatcttgtc caggtcgttc
     6121 agatcgaacc cgatcttctc tgccgccata gtcgcagcct ttgccagagc gtgtgtggca
     6181 aacttgtaca cctctctgcc gtcctggagc atagcgtgtt cccagccctc cggagctgcg
     6241 ccgatgccgt cgtcatactc tgccagacgt tccggcttta cgaacggaga ctgaggcagt
     6301 gcgctgtggg catagaggaa ctttgcgccg gtgccgtctg cgcccagatg gctggtgtac
     6361 agcgcatctt ccttgtattc gatgagcgca gcagctgcgc cgtcaccgaa gaggatgcag
     6421 gaggaacggt cattgtagtt ggtgatcttg gacaggctct catttgccac caccagggca
     6481 tagcgcatag tagggtctgt tttcagaaaa cggtgcgccg tgtccatagc atagacaaag
     6541 ccggaacaag cggcattcat gtcaaagcag gcagcattgg ctgcgccggt ctcccgctgg
     6601 atgatgcagg acatagacgg cgtgcagtac tccggcgtaa tggtagtgtc gatcagcaga
     6661 ccgatctcca gcggatcaat gcctgccgcc tcgatggcac gcttggcagc ctgtgcgccc
     6721 atataccagg tgggttctcc gtcggagatg tggcgtgtgc cgataccggt tctggttctg
     6781 atccattcgt cgttggtctc gacgatcttt gccatatctt cattggtcac ctgaaatgca
     6841 ggcgtatagc tccctgttcc taaaacatga attcccatgg aaaaaattcc tccgttcctc
     6901 ttgtcacttt cctcattttg tggtatgata gatagggaac gtaccgcaga cagaggtttc
     6961 ggcacgccct gtaaaattta gatttcccct gcaatcacag gatacacttt ctattataga
     7021 atatttttac gcaatttgca atgtttccgg gtggtttttc aagtcccgaa agaggaaata
     7081 ctatcttttg cacaaattca agaggcttat tttgtacatc tttgtacact cttatgagaa
     7141 aggaatggtc gttatggcaa caatcgcact tttttccgga cagggttcac agtatgagaa
     7201 gatgggactg gatctggtgc agcaggacgc tgcgctggag gaaattttcg aggtcgcatc
     7261 cgaggtgctg ggctttgacc tgtatgcagt gatcgcagac agcgacgccc agacactggc
     7321 gcacaccatc tatgcacagc cggcaattat ggcaacctct ctggtctgcc tggaggcagc
     7381 caaaaagcgt ggctttacct atgacgccgt tgccggtcac tctctgggtg aatatgccgc
     7441 aatggtagcc gcaggaatgc tctccctgga ggacgggttc aaggtcatca aggctcgttc
     7501 agaggcaatg gatgccgcag caaaggcagc agacggcgca atgtgtgcca ttctgaaact
     7561 tgccccggca gaagtggagc gcatctgcag cgagaccgag ggctatgtta tcccggcaaa
     7621 ctacaattcc agcgtacaga ccgtgatcgc aggcgaccgg gacgctgtgg aaaaggcagc
     7681 tgctgcctgc gctgcgctga aggcaagagc agtgccgctg gctgtggcaa gcgcattcca
     7741 caccaagctg atgcagccgg ctgcggatac cttttataaa gcaatccagg atgtgaagtt
     7801 ctccgcaccc gctgttccct tctactccaa cgtgctgggc gcagtccgga cagacttctc
     7861 cgatatgccc aaggcgctgg cagaacacat cgtctctccg gtgcgcttta ccgacgaact
     7921 ggcagccatg caggcagccg gcatcgacac ctttgtggaa ttcggtccga agagcgtcct
     7981 caccggtctg gtgaaaaaga ccctcaaggg cgttaccgca aagaatgtgg aaaactgtga
     8041 gacactggaa ggagtgctgg cataatggaa cagtacacac tgatcggaca tcccctggga
     8101 cactccatgt ccccgtttat ccacgagcgc ctgtttgcca tggcaggcag agaggcaagc
     8161 tatacgctga cggatattgc tccggagcat ctggagggca aggagccttt cctgcgcagc
     8221 ttacagggct ttaacattac cattccccac aagatggcaa ttatccccat ggtggacgaa
     8281 ctggacgaaa gtgcaaagcg gtacaactcc gtgaactgcg tggcgaacca gaacggcaag
     8341 ctggtgggct ataacacgga ctgcgacggc ttcaccatgt cggtgaagga ctatcccatg
     8401 gacggcaagg tcctgctcat cggctgcggc ggcgtgggca gaatgatcgc caccgaggca
     8461 ctgcgccacg gggcagatct gaccatcggc atcattcccc aggacaggga actggtggaa
     8521 gagtttatcc gagaggcaaa gcagaccatg ccggatgcca aggtacagta tgcggtgacc
     8581 gcagacatcc acgacaagtt cgatgtcatc atcaacgcat cgccggtggg aatgttcccc
     8641 aaggtggacg cctgcccggt atcggacgag ctgatcgacg gcgcagacta tttcttcgac
     8701 gtgatctaca atcccaccaa gaccctgttc ctgcgcaagg cgctggaaaa aggcaaggtg
     8761 gcaaaaggcg gcgcagccat gctggtactc caggcagtcc gggcgcacga gatctggaac
     8821 gatgacagct acagcgatgc gcagatcgag tccatcatct gcgacatgga agaactgatc
     8881 gacgaacaga acgccaaaaa gtgagggaca gcatgacgat ttttctttgc ggttttatgg
     8941 gctgcggcaa gaccacagtg ggcgagatcc tggcgaaaaa gctggggctg ccgctgatcg
     9001 acacagacgc ttatatcgta gaacaggaag gcaagtccat cccggacatt ttcgcccagg
     9061 acggcgaacc gtatttccgg aagaaagagg ctgaggcgat ccggacgctc tgcacacaga
     9121 atgctgtgat ctcctgcggc ggcggcgcaa tgctgaaccc ggacagcgca gactacgccc
     9181 ggaaaaacgg cgcagtagta ctgctggacg agtcctttga cacctgctat ggacgcatcc
     9241 agggcgacac caaccgcccg atcgtccagc gcagcaccag ggatgagctg caccagctgt
     9301 tcgacaagcg ctccggcacc taccgggcgc acgcctctca cgtgattccc ggcggcgttt
     9361 cgccggaaga gatggcagaa cggatcatgg cggcagtaaa ggcataaaca taacatagaa
     9421 aacgctctgt attcggcact gtttccgaat acagagcgtt ttttctttag gcaacaccgc
     9481 acagagtttg tgcgtaagat gcgcagtcgg atttttcggc agattgcgtg caaagccgtc
     9541 aaacgggctt tgtgcggtgt tgcctttaat cctcttcgta caccggcagt tccgccgggt
     9601 tgtatgggat gtggtttcgg tcgtaatagt cccgaacagc tgcccggact gcctgctctg
     9661 ccagcacgga gcagtgcagc tttttctccg gcagaccatc cagcgcctcg gcaactgcct
     9721 cgttggtcag cgccagcgcc tgggacagcg gctttccggt aatcagcacc gtcgccatgg
     9781 aactggacgc aatggcagag ccgcagccga aggtctcgaa ggtggctttg gtgatgatgc
     9841 cgtcctcgat cttcagatag atcttcatgg agtcgccgca ccgtgcgctg cccacctgtc
     9901 cgacaccgtc cgcatcctcc atcgctccca cgttccgggg attcataaag tgatccatga
     9961 cctttgcgct atataacatg gggctttctc ccttcttcca gatcacgcca caccggcgac
    10021 attccacgca gatatgccac cacttccggg atggtctccg tcagatattc gacttcctcc
    10081 ggggtgttct cctccgacag cgacagccgc agagagccgt gcgccgcctc atacggcacg
    10141 cctaccgcca gcagcacatg gcttgggtcg agggagccgg aagtacacgc cgaaccggaa
    10201 gaggcacaga tgcccttggc atccagcaga agcagcagtg cctcgccctc aatgccctca
    10261 aagcagaagt tcacattgcc cggcaggcgc tgttcccggt cgccgctgag gatgctgtgg
    10321 ggaatccgct ccagtccggc gatcagctta tcccggaggt ctgccacata ggcagcgttc
    10381 ttttccaggt cggcacacgc cgctttcagc gccgccgcca tgcccacgat cgccggcagg
    10441 ttctccgttc cggcacgctt gctgcgctcc tgtgcgccgc cgcagatgag actgcgcagc
    10501 ggtgtgccac gcttggcgta cagcacgccc acgccctttg gtccgtggaa cttatgcccg
    10561 gacagggaaa gcatatccac cggcagtttc gccaggtcta ccggaatatg ccccaccgcc
    10621 tggacagcgt cggtatggaa cagcacgccc ttttcccggc agactgcgcc gatctccgca
    10681 atgggttcga tggtaccgat ctcgttgtta gcaaacatca cgctcaccag gcaggtgtcc
    10741 ggtcggatag cggcggcgac ctgctccggg gtgacgatac cggttttgga gacctccagg
    10801 agcgtcacgg aaaagccctc ttcttccagc tgtttcagcg tgtggagaat ggcgtgatgc
    10861 tcgatcacgg tagagataat gtgttttttg ccctcttttg caccggcata ggcggcagag
    10921 cgcagcgcct ggttgtccgc ctcgctgccg ccggaggtga agtaaatttc ccgtgccgaa
    10981 cagcccaggc aggcggcaat gtcctggcga gcctggagca gtgcctcagc agctgcctgt
    11041 ccgatgtggt ggacgctgga gggattgccg tagttctgtt ccatgcagga caccatggct
    11101 tggattgctg ccttgcaggg ctttgtggtg gctgcattgt ctgcgtatat ttgcaatggg
    11161 attcctccgt ttctggttga aaatgtcccg gtgggaacac acaaatccaa ttttataatt
    11221 gtttctctta aaaaaatatt tctcacgagg tagcctttta aggctcccct aacaggggag
    11281 ctggcaagcc gtaggcttga ctgaggggtt aatcc
//
LOCUS       DAWENO010000059        11301 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2430_length_11301_cov_12.608394, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000059 DAWENO010000000
VERSION     DAWENO010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_11301_cov_12.608394"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8947..8956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9273..9282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2544 a         2869 c         3629 g         2239 t
ORIGIN      
        1 caatggtgag aaccgaagaa ctgaatacca tcgaggatct gtacgcctcg acggaagaga
       61 tgctggagct gccgatcccg gaaaaggtga agcagtatgt gtccggcgtg atgtcccaga
      121 cagaggaact ggacagcatt attgcagcgc acagcaagca gcgcagtctg aaccgtgtgc
      181 cggcagtgaa ccgttcgatc ctgcgtctgg cactgtacga attgaagaat atgccgcaga
      241 ctccggtgaa tgttgtcatc agtgaggcag tgcatctgtc ccaggcatac gcatatccgg
      301 aggacaccgc ctttatcaac ggcgtactgg gtgcatatgc acgttccaag ccggcggcaa
      361 aggaggacac tgccgatggc acaaattctg gggattgata ccagcaatta caccacctca
      421 gcggcgctgc tggatctgga gaccggagag gtacaccagg aaaagcagct cttgccggta
      481 aaagtcggcg aggtgggact gcggcagagc gatgcggtgt ttcaccatac acgtcagctc
      541 ccggaactgc tggaacggct gcgcccgttt ctggagaata atccggtctg cggcgtcagt
      601 gtttccacac gtccccggaa tgtggacggt tcgtatatgc cctgctttct gaccggtatg
      661 ggaactgccc gtgccgttgc tgcggtgagc ggtgtgccgc tgtatgagac ttcacatcag
      721 gtgggtcatg tgctggcggc gctgtactct gccggaaagc tgggggaacg ggaaaagccg
      781 tttctggcgt ttcatgtcag cggcggcact accgattcgc tctactgcga gccggatgcg
      841 gatatggcgc tgaagatcac gccctgcggc acttcgctgg atctgaaagc tggacaggcg
      901 atcgaccggg tcggcttgat gctgggcttg cagtttccct gcggcaaggc actggaacag
      961 ctggcggcaa agagccggaa aacattccgg atcaaaccag tggtgcgtgg catggactgc
     1021 tgtctgtccg ggctggaaaa ccagtgccgg aaacgcctgg aacagggcga accgccggag
     1081 gatgtggcaa agttctgcct gctgactgtg gcggaaactg tgatcgccat gacaaaggag
     1141 gcacaggagc gctatggcag gctgccggtg ctgtatgccg gcggcgtcat gtctaaccag
     1201 ctgatccgtc cgctgctggc ggaaaatgcg gtgtgctggt ttgctgcgcc ggagttcgcc
     1261 tgtgacaatg ccgcaggtgt ggcggtctat ggtgcgtggc gaaagcaagg aggaaaactg
     1321 tggcaatttt aagcgtgagt cagctgaacc gctatgtggg attcaagctg aaagaggatc
     1381 gtgcgctgca gggcatcttg gtgcgtggcg agatcaccaa tttcaccaac cactaccggt
     1441 cggggcatct ctattttacg ctccgggatg cggaaagctg tgtcaaggcg gtgatgttcc
     1501 ggagcaatgc ccagcgtctg cggttcacgc cccgggaagg gatgaatgtg atcgcagcag
     1561 cgacggcatc gctctatgaa cgggacggcg catttcagct gtatgtgaca gatctccagc
     1621 cggacggcgc tggcacacag gcgctggcac tggaacagct caagaaaaaa ctgacggcaa
     1681 tgggtgtgtt cgatgcggcg gcaaaacgtc cgctcccggc aatgccccaa aagatcggcg
     1741 tcattacttc ggacaccggt gcggcgcttc aggacgtgaa aaacgtcatc gggcggcgct
     1801 atcccatcgg acatctgctg gtctatccgg cacaggtgca gggtgatgcg gcggcggatt
     1861 ctgtctgccg tgctattgcg gcggcagagc gtgacggctg tgacgttctc attgtgggac
     1921 gtggcggcgg cgcatcggag actttggagg cgttcaacac agagcgtgtg gttatggcga
     1981 tctatcactg tgctgttcct gtcgtttcgg cggtgggaca tgagacagac tggacgctgg
     2041 cagatgcggc ggcagacctt cgtgcgccta cgccgtctgc ggcggcagaa ctggctgtgc
     2101 cggatgtgcg ccatctgatg cagcaggtgc aggagcaggc acgccggctg gacagcgcca
     2161 tgcagcagca gctccggcaa aaggcacagg aactggaacg gctgtcagac cgcttaaaga
     2221 tgcagtcgcc ggcgcatcgg caggagttgg cgcagcagga actggcgcat ttgcagcagc
     2281 gtctggaaaa aggaatggcg ctgcggctgg aacagtgccg ggaacagctg gcacagcagg
     2341 cggcacggct ggagatgctc agcccgttga aaattctggg gcgtggttat gccctggcgt
     2401 atcacggcga ggaactggtg cgcagtgccg cacaggtaca gcccggagac cggctgcggc
     2461 tgcggctggc agagggcgag gttatgacag aagtcgtacc acaggaatca taaacagaac
     2521 agcaaagcac aaagcttgtg cggtgctgta tcgaaatatc ataggcgaat ttcgggaaag
     2581 gagaggatgc acatggcatt tgagacacac atgaaagcgc tgcgggaact gacgcagaaa
     2641 ttggagcagg gcaacctgcc tctggaagag gcggtacagc tctacagcaa gggcatggaa
     2701 ctggcggcat cctgccagga ggaactgcaa acggcaaagc tgcagatctc caaacagaat
     2761 gtgccggcgg cagaacagga ggaaacagat ggaagcgaag gataaagccc atttggaaca
     2821 gttggcggca gaggtggaga cacagctgct ccgctatgta caggtgacgc cggagacaaa
     2881 gcgccaggcg caggtagtag atgccatgcg gtattctctg gaggcaggcg gcaagcggat
     2941 tcgtccggtg ctggtgctgg aattctgccg gatttttggc ggcacaaagg aacaggcgat
     3001 ggcggcggcg tgtgcgctgg aaatggtgca taccttttcg ctcatccatg acgacctgcc
     3061 ggagatggac aacgacgatt accgccgtgg aaagccgtcc tgccacaagg cgttcggcca
     3121 tgcggtggct ttgcaggcag gggatgccct gctgaccgag gcgtttcgtg tgatcgtgga
     3181 tgacccgatc ctggaaccgg aaatgaaagt ggagctgctg cacattctga ccgaggcgac
     3241 cggcgccgcc ggaatggtgg gcggacagat catggatatg gcaaacgaaa agcgcagcga
     3301 cgtgaccctg gaagagcagg aggcgatgtg tgcggcaaag accggcgcac tgatccgtgc
     3361 cgcctgccgc ctgggctgca ctgccggatt tgcgtccaaa gagcagatca tactggcaga
     3421 ccagtatggc gcaaagctgg ggctggcgtt ccagatcgtg gatgatatcc tggatgtgac
     3481 cagtacatct gaggtgctgg gcaagccggt gggcagtgat gccgaagagg gaaaaacaac
     3541 atttgtaacg ctgctgggac tggaagaggc gcagagacgt gctgcggctc tgacagaaga
     3601 ggccgagcag attctggcgc agttcccgga acacgagttc ctgatggcac tgacgcagga
     3661 gctgctgcgg cgtgaaaaat aaataaagga gtgaagtctg cgggaatgtc acggattttg
     3721 gctgtgacag gagactcgca gacaatgaag aaatggagca taatcaattg ccgaaggaag
     3781 atgcgccgct gttggaacgg ctgcattctc ccaaggatct gaaagggctt tcgctggaac
     3841 agtgtgagtc gctctgtgag gagattcgaa gtctcctggt ggagaccgtc tcgaaaaccg
     3901 gcgggcatct cgcctccaac ctcggttccg tggaactgac ggtggcgatg caccgtgtat
     3961 tttcctcgcc ccaggacaaa tttgtctggg acgtgggaca ccagtgttat acccataaaa
     4021 tattgaccgg acggctggag cagttttcta ccctgcggca ggaaaacggc atctccggct
     4081 ttccgaaacc aaacgaatcg gaacacgata gctttatcag cggtcacagc agcacggcga
     4141 tctctgttgc ctgcggcatt gcggaaggaa tgcgtcagca cggagacaga gagcattttg
     4201 ccgttgcagt agtcggtgac ggcgccatga ccggcggtct gtcctatgag ggactgaaca
     4261 atgccggcaa gtcgggaaac aatctgatcg tgattctgaa cgacaacgaa atgtccatct
     4321 ccaaaaacgt gggagcgctg gcgagatatc tctcttccat gcggagcagt gagggctatg
     4381 tccgcaccaa aaaggcggtg gagcgccgtc tgaaccgcac ggctgtgatc ggtccgtctg
     4441 tggcgaaggt catcaagaat tccaagagcg ttgtccggga tgtgttgctc cagtctgcca
     4501 ccatctttga ggatttcggg tttgtatacc tgggaccagt ggatgggcat aacctgggcg
     4561 acctggaaga ggcactgctg gcggcgaagg aatatcaccg cccggtattt ctccatgtgc
     4621 atacggtaaa gggaaagggc tatgcgccgg cggaggaaaa tcccggcgaa taccacggca
     4681 tttccaagtt cgatattgaa accggcaacc cggaggtctc cgggacggat tcctattccg
     4741 atgtgttcgg caaggaactg gtgcgcctgg cagaggagga ctcccgtatc tgtgcagtga
     4801 ctgccgctat ggaacacggc acgggactgc atcattttgc acgaaagttc ccgaaccggt
     4861 actatgacgt gggcattgcc gaacagcacg ccgtgacatt ttccgccggg ctggcatcca
     4921 tgggagaact tcctgtattt gcggtgtact cttccttttt gcagcgtgcc tatgaccagc
     4981 tgatgcacga tgttgccatc ggcggactcc atgtagtgct gggcatcgac cgtgccggtg
     5041 ttgtgggcga ggatggtgag acccatcacg ggctgtatga cgtgtcgttc ctcagcacag
     5101 tgccgggcgc tgtgatctat gcgccggctt gttatgacga actgcgtctc tgtctgcgcc
     5161 gggcgctgta ccgggacaag ggactggcgt gcgtgcgcta tcccagagga aatgacaaga
     5221 gcgtgtttga caagagcgcc ctgaacacgg agtataccca tgtttccggg aaaaagaccg
     5281 atatcctgta tatttcctac ggcagagtat atgataatct gtaccgtgcg gcaaagcgct
     5341 gtgcagagcg ggagcttcac tgtgacctgc tgaaactgac ccgtatcttt ccgctggcgg
     5401 atggtgtggt ggacattgcc atgtcttatc gccacgtggt attcctggaa gaggcgtatt
     5461 attacggcgg catttcccag ctgttcggcg atctgctgct ggaacgtgga ttccaaggta
     5521 cataccgccg ggtcgcaccg aaacagtatc tgctccaggc atctacggat tcccagatgg
     5581 agaccatggg gctttcagag tacgccattt accgggatat ccggcaggag gcagagcatg
     5641 gcgagacttg atgcggcaat ggtgcagcag ggactggttc ccagccgcca gcgtgcaaaa
     5701 acgctgattc agaacggaca gatctctgtg aatggaaagg tctgcaccaa gccggcattt
     5761 gctgtggagg agaccgatca gatcaccatg gagggcagtg atattccttt tgtgggacgt
     5821 ggcggtctga aactggaata tgcattggaa cagacaaagc gttctctgga cggactggtc
     5881 tgtctggata tcggcgcatc taccggcggc tttacggact gtatgttaca gcgtggcgca
     5941 gcaaaggttt acgccattga cgtgggacac ggacagctgg tgcagtcgct ccgggaggat
     6001 tcccgtgtgg tgaactggga aggcacagat gtccgcagca ttgcaccgga acagctggaa
     6061 cagccggcag actttttttc agtagatgtg tcgtttattt cgctgcacca tgtactgccc
     6121 tatgtgctgc cgctgctgaa accggatgct gccggagcaa ttcttatcaa gccccagttt
     6181 gaggcaggca gggaaaacat cggcaaaaac ggactggtgc agtcgcagcg tgtccatgta
     6241 caggtactgg ggaatattct gacgctgctg tctgcgctgg gactgacggt gcagctgctg
     6301 tgtccgtcac ccatccgggg tggttccggc aacgtggaat atctggcggc agtgcagaaa
     6361 ggcggcgaaa cggcatatgt gccggacgct gcggctgtgg tgcagcaggc gtttacacag
     6421 gcgaaaaagg agaagacgaa atgaaggtcg tgttgtttca ccatctgcaa aagggcaatg
     6481 ccccggaagt ggcgagaaag gtctgcctgg cactccatcg gaacggtatg gaggtctggg
     6541 cagacccacg ctgtctgggt ctgctgaaag atatggattt cgtgtgctat gcgccttttc
     6601 tggaggcgat acaggatgcg gatgtggcag ttgccatcgg tggtgacggc acgatcctgc
     6661 actgcgcccg tcatgtggtg ggaacaagcg ccaagctgct gggcatcaac accgggcggc
     6721 tgggctttct ggcatccatg gagccggatc agatcggcga gatcgcacgg ctgcagaccg
     6781 gagactaccg ggtgtccaag cggatgctgc tccgaggcaa ggtgctggac aaaacaggga
     6841 cttgtctccg ggaactgacg gcgctcaacg acattgtcat ttcccggaat ttttcccgtg
     6901 tcattgagtt tactgtgtcc cggaacaatg tggtgctggg cgaataccgg gcagacggcg
     6961 tgattttcag tacgcctacc ggttctaccg cctatgccct ttccgcaggc ggtccaatca
     7021 ttgagccgga gttttcctgc attgagatga atctgatctg tccccactcg ctgtcggcga
     7081 gacctatctt gttttcgccc cacaacgacc tgcaggtcag tctccgtgtc cgggaggaat
     7141 acgatgcgta tttctgcgca gacggggaaa agccggagcc ttttccggag ggcagagtca
     7201 tggagatctc cggctctaac cagtcgctgg agattctgga tctcagcgga aatacatttt
     7261 ttgattccct cagcagaaag ctgatgcagt cactgaaatc aggaaggtcg tgagcagaac
     7321 gatgaaaaag aaacggcagg acaccatttt ggcgctgatc gctgcggagg atatcccgac
     7381 ccaggaggtg ctgcgggagc ggatggcgga acagggcttt gcggtgaccc aggcgacgct
     7441 ttccagagac atccgggagc tgaaactgac caaggagcgg cgcagcggcg gcagagcctg
     7501 ctatattgca ccggtgcggc aggaagtcaa cggcggcatt ttgcagaatg ctgcactgcg
     7561 gacggattat gccggaaacg tttctgtgat ccactgccgg acaggcacag cacaggcggt
     7621 ggctgcggca ctggatgcca tgcaccggga agatgtggtg ggaacgatcg ccggagatga
     7681 taccattttc gtactgctgc gcacagaaac gcaggcaaag gaatttgccg gcagctttgc
     7741 gagactgatg gaaacaggag gattctaatg ctggctgaac tggaaatcga aaatctggcg
     7801 gtgatcgccc atgcacgcat cccgttttca ccccggctca acgtgtttac cggtgagacc
     7861 ggcgccggaa agtccattct gctgcacggc atccatgcgg tgctggggca gcgtgtcaca
     7921 cgggatattg tgcgcaccgg ctgcaaaaag gcagtggtga cggcgctttt cacccacctg
     7981 gatccgctgg tctgtgcgag actggatgcg ctttccattt cccatgtgga cgatgaactg
     8041 ctgctgaccc gtgagatcgc tgcagacggc ggaagcgccg cacgggtcaa cggacatacc
     8101 acaacggttt ccgttctccg ggagatcggc gatgctctta tcaacatcca tggacagcat
     8161 gacaaccagg tgctgcttgc gccggaatgt catctgcaaa ttctggattc cttcggcgga
     8221 gacgatactc tattgcagga gtaccaggag acatttcgcc agctgcagca ggtagccaag
     8281 cggctgaacc agctggtaaa gcttgaacag gataaggcgc accgccggca tcagctgaaa
     8341 gcactggttg ccgaggtgga cgagctgggg ctggaactgg gcgaagagga tgccctggaa
     8401 gaagagatga accttttgca gcacgccgaa agtattgcgg cggcactgca ggcggcggca
     8461 gatgccattg acggcgagga tgccgacggc tctgcggtgc tgctgaccga ggcggcagca
     8521 gaggaattgt ctccgttcgc tgatctgcgg acggagtttt ctgtgctggg agagcgtctt
     8581 tccgcagccg gcattgaact ggcggacatt gcgtcagaat gccgccggtt ccgggacgac
     8641 atggaactgg atccccagcg ctatgccatg gtgcagaaac ggctgctgga gatccggcgg
     8701 ctcagccggg agtatcagtg taccggagac gaactggtgg agcgccgcaa ccgtgcggaa
     8761 gaggaactgg aacagatgca gggcgctgcc gcagagatca ctgcgctccg ggagcagaag
     8821 gcggaacttt tggaaaaggt ctccgtccag gcgaaggcac tgtcagaata ccgctcggag
     8881 acactgcagc gctttacggc agaagtgacc gaacagctgg aatttctaaa tatgccgaac
     8941 gtggtgnnnn nnnnnnttct catttcggcg aaccggggcg aagaaccgag acctctggca
     9001 cgcatcgcat ccggcggtga actgtcccgt atcatgctgg cgctgaaatg cgttattgcc
     9061 gaccgggaca gcatccccac gctgatcttc gacgagatcg acaccggcgt cagcggcaag
     9121 gcggcgcaga agatcggcat gaaactccgg gaagtgggac agctgcggca ggtgctctgc
     9181 gtgacccatt tgtcccagat cgccgttatg gcagaccagc atctgatgat cgaaaagcag
     9241 accgagggga cgagaacgga gactcatgtt atnnnnnnnn nntcatgtta tggtgctgga
     9301 accggaaggg cgtgtgcatg aaattgcccg gatcatgggc ggcgaaaatc ccagtgtgct
     9361 gctgctggag acggcaagag aagaattgag acgctggcag gatgacttgc tgccggagag
     9421 agaggacgga caggaaacat gatacagcca gaggcgctgt tacagcaata tttcggacat
     9481 aaggcatttc gcaccggaca gaaacaggcg atcgacgcca ttctttccgg cagggatgtg
     9541 ctggcggtca tgcccacagg cgcaggaaaa tctgtgtgct atcagatctc ggcactgctg
     9601 ctgccgggcg tgacgatcgt catttcgccg ctgatctcgc tgatgaaaga ccaggtggag
     9661 gcactccggc aggtgggcat tccggcggcg tacatcaaca gcagcatcag ccaggaggag
     9721 ttctatcatt ccatgcagat gttacagcag ggacagtgcc gcattctcta tgtggcaccg
     9781 gaacggctga tgacggagag tctgctccgg ctggcgcagc gggtgcagat cagcatgatc
     9841 gctgtggatg aggcgcactg cgtctcccag tggggacagg atttccggca gagctatctg
     9901 gacattccgg tgtttatgga acagctgctg gtgcgtccgg tctgcacggc gtttacggcg
     9961 acggcaacca agcaggtcga ggcggatatt gcacgcattc tgaagctccg tgacccggag
    10021 accatacata ccggattcga ccggaaaaac ctgtttttcg gagtacgccg tcccaacagc
    10081 aaaatgacgg aactgtttca cctgctgaaa gaaaacgacg gaaaaagcgg cattgtctac
    10141 tgctccacca gaaaggcggt agaagaggtc tgtgcatctc tctgtgatgc cggattcgat
    10201 gccacacgct atcacgccgg actttcggac gtggaacggg cgcagaacca ggacgatttt
    10261 ctctacgacc gcaagcccat tatggttgca accaatgcgt tcggcatggg catcgacaag
    10321 tccaatgtct ctttcgtcat ccactacaat atgcccatgg atctggaaag ctattaccag
    10381 gaggcaggcc gtgccggacg ggacggcgaa ccggcgcagt gcatcctgct gtacagcggc
    10441 agagatgtgc ggacaaacga gttcctgttg aaacgcagcc gggagaccat ggatgtggac
    10501 gatgaggaga cccggcagtt tctgctgaac cagggaatgg agcggctgaa acagatgaca
    10561 ttttatgcca cttcgacctc ctgtctccgg catcggatgt tgcggtattt cggtgaccat
    10621 gcaccggaga gctgcggcaa ctgttcctgc tgtctcacca actaccggga agaggatgcc
    10681 accatggcgg caaagaagat catctcctgt gtctatcggg cgcagaaagg cggttatcat
    10741 ctgagccgca ccatgaccgc cgatgtgctg atcggcagca aaaaggaaag cctgctgcgg
    10801 atgcggctgg atcggctgtc cacctacggc attatcgaaa agctgacgca gaaagaagtg
    10861 gtggggctga tcgatgaact gctccagcag gaaaatctgg cgctccgccc gtttcaggag
    10921 taccaggaac tggcgctgac tgccggatcg gtggagatca tccgggatca gaaaaaggtg
    10981 atgcgccgtg ttcccattgt ccgggaaaag ctggcggcgc cggtgggaac aaaagacccc
    11041 actctgtctg cggcagacaa cgaactgttc caaaagctgc ggcaagtgcg gtcggcacag
    11101 gcaaaacacg agggaattcc gccgtacttt atcttttccg atgccacgct ccgggatatg
    11161 tgccggaaaa ggccccggag catgggcgat atgcgccagg tctccggggt cggtgtcatt
    11221 aaggcacaga agtacgggaa gatgtttctg gaggagattc agtcgtttca gccggaagtt
    11281 tcagtttgat ttttatgcaa a
//
LOCUS       DAWENO010000060        11113 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2481_length_11113_cov_14.045849, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000060 DAWENO010000000
VERSION     DAWENO010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_11113_cov_14.045849"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2727 a         2883 c         3282 g         2221 t
ORIGIN      
        1 caccaggatg aggcagttca cagcggcgct tatcagaacg gcagcaccta cggcaatccg
       61 gcaggcagca atacatccaa cgtcaacaac accgtatctg ccggaaacgg cggcaacggc
      121 aacaacggcg gttattccta tgtgaactat tactccgacc ccaacggcgg cgaccccaac
      181 aaaaagccga agaaaaagca caccggtcta aagattgcgg cttttgttat ggcgatggtt
      241 ctggtctccg gcggttcgat cggtgtctat gagggcatcc gctctgccaa tgctgacaac
      301 ggcagttcta ttgtggcaag caatgattct tccgcagcag atgaaagcca gagcaaaaag
      361 tcggacagcg attcttccaa gaagagcagt tccgatcaga gctggatcca gctggcatct
      421 accaacggtt cgatgtcggt agcagatatt gttaagaaag tgaccccgtc ggttgtcggc
      481 gtacagtcca cgttctcttc ttccaacggc aataccaaca ataacagtta cggcggtttc
      541 ttcggctacg gcaacggaca gcagggcaac agctcgcagg gcatgaccgg cgtcggcact
      601 ggcatcatca tgtcgaagga tggctatatc gttaccaatg cacatgtgat ttacgacgat
      661 gagtacggtt acggcgaggc atcttccgta cagattcaga tgtcggatga gaccaccact
      721 tatgatgcac gcatcgtggc atacgacaaa gaggcagata tcgctgtact gaaaatcgat
      781 gcagacaatc tgactgccgc agaatttggc gacagcagca gctgtgaagt gggtgaaatg
      841 gtagttgcta tcggtaaccc gctgggactc cagttccaga atacggttac ctgcggcatc
      901 atctccgcac tggaccgtaa ggtcaccatc aacgacaaca ccatgacgct gatccagacc
      961 gatacggcga tcaacaacgg taactccggc ggtccgctca tcaacagctc cggtcaggtt
     1021 atcggcatca actctgcgaa gatgtccagc acctattccg gtgaggcaac tgtagaaggt
     1081 atcggctttg ctatcccgat gtctgaggcg aagagcatca ttgacgattt gatcaactat
     1141 ggctatgtga ccggccgtcc gcagcttggc atttcctgcc aggatgtgac agaggcagtt
     1201 tcacaggctt acaatattcc ggtcggcgct tatattttca gcgtcaccag cggcggtgca
     1261 gctgaccagg ctggtctgca gccaggcgat gttatcaccg gcatccagga tcagaccatc
     1321 acgaccaccg aagaactgaa cgcagtcaag aaccagtaca aggctgggga taccatcacg
     1381 ctgacctatg tccgtgccgg cgagaccaaa aaggtcgatg tcatactggc agaagtacag
     1441 cagagtgaga ataactaatc attcgggtac tgcgttattc aattgacatc ttgttttttt
     1501 catacattct ccttattatt tttcgggaaa gcgtccgaca gtttctgtcg gacgtttttc
     1561 tgttacaacg gctgttttcc acgtatcttt ttgcagcctc acgcaggatt ttcacgtaat
     1621 ttccataaac atatgcaata ataggaacat agaaaaacag aaaccacgct tggggaattg
     1681 ggtatcacat acgaggagtt ttgacaaatg acaaattcac atcatggggt accaaataca
     1741 aaaaggagcg tgagtctcat gcaaaagaaa atatttgcct gggcaatgag catggcggta
     1801 gcggcgatga tcgcctgcac cggatgcaca caaaacagca gcacatccag cggcacggac
     1861 agcgaaacag cacagacgac cacgctggag gaaaccgacg gttcggtcag cagccagaat
     1921 ctttctgcgg tgcagtccgg aactgccggc gtgaccaccg atgcaggcga cctttgcgac
     1981 acttatgaca gctttgacgc agagatcgct ctggcagacg atgataccaa ggtcaccggc
     2041 aatacaaaag ccgtttccgt aaaaaataac cgggtcacca tttccgcagg cggcacctat
     2101 cgtatcagcg gcaagctgac aaagggacag gtagttgtca ccggttcgga aaaggtgaag
     2161 ctttatctgg acggcgcaga gatcaccagc acatctggtc cggcgctggt gtgcgtcaat
     2221 gaaaagcgca ccgtcctgtc actggcgaag ggtacgaaaa acgttttgac agacagcgcc
     2281 gacaacgccg aaaccacaga gagcggaaac aacgtggatg cctgcgctct gtttgcacag
     2341 gataagctga cgatcaacgg cagcggcagc ctggcagtct ccggaaacag ccgggacggc
     2401 attgtctgca gggatacgct gaaactggtg aatggtacga tcaccgtgga tgcggcagag
     2461 gatggtgtaa agggcaagga ctgcgtggca atgttcggcg cagatctgac tgtgactgcc
     2521 ggcaatgacg gtgtaaagtc caccgaggac agcgatgccg caaagggctt tttgcagctg
     2581 acggacggca gtgccgcagt gacagccggc ggcgattgtc tccaggcaga gtctctggtg
     2641 tgggtgacag acggtacgta taccctcacc agcaacggca cggctgtgga tgcagagacc
     2701 ggcgagacct ccagcagcaa aggtattaag tgcagcggtg atgttgaaat cgccggcggc
     2761 acactgacca ttgatgcggc agaggatggc gtgaactgcg gcggcgctat ggagatacag
     2821 gacggcgaga tgaccgtttc cagtgccgag gacggcatcc aggcagacgg tgacctcacc
     2881 atttccggcg gcactgtcca ggtgacgacc accggagaag tggcagcctc tgcacaggac
     2941 gacttccagc ccggcaattt tggcggcggt acaccgcctt ccggtgaaat gccctctgga
     3001 gatgcaccct ccggaaatcc gccggagctg ccggacggcg agacctttgg cggcggcaat
     3061 ccgcccagcg gaaatgcacc ttccggcgat gttcccggac agaacgggca gaatgccaac
     3121 gccgaaaacg ctgacgtcat ccaggcggca gccgttcaga cggataccac tgcggcatct
     3181 gtgacagacg cagcagattc gcagacaaca acaaccacaa caacggcaga tgatgccacc
     3241 agcaagggca tcaagtgcgg cggcaatctg gtgatgagcg gcggcagctg taccattcac
     3301 tccacagacc acgctgttca cgctgccgga acggcagaac tctccggcac aacgctggac
     3361 attacttccg acaacaaggg catcagcagc cacggtgatc tcaccgtttc agacggcagc
     3421 attaccattc acagctgcac cgagggcatt gagtcaaagg cggaaatgaa catcagcggc
     3481 ggcgagatac ggattctgga tgccaccgat gacggactga acaccggcag cgcagacagt
     3541 ggctcccacg ccatgaccat ttccggcggc tatatctacg tcaacgcctc cggtgacggt
     3601 gtggattcca acggcacatg gacaatgacc ggcggcacac tgctggtgtg cggtccgacc
     3661 agcggcggag acggctcgct ggatgccaac ggggatatga cctataccgg cggcacactg
     3721 ctggcgctgt ccagcaaggg catgatggaa tatccggaga gcggctgtct ggtggcaacc
     3781 aactgcaacg cagcagccgg cgaccagatc agcattgtgg atcagaacgg tacggttctt
     3841 gtgacactgc agtcgcccaa ggcggtctcc gatgtgatct atggcatcgg tgacggcgac
     3901 tcgtcggatt ataccattgt gaccggcggc acatattccg gcacgctgaa cgatgacggc
     3961 tatgccgagg gcggaacggt cagcggcggc acggaagtga cagcaaacgg cggctccggc
     4021 ggcatgaacg gcggtcagcc cgatggaatg ccttcgggac agcccggcgg acagggcggc
     4081 acaccgccca gcggttctaa cggcggcacg cctccggaaa agccgggaca gaacagcacg
     4141 gacagcagca cagaaaactc ctgaccagac accatccgat tcaaagtagt ctttcacata
     4201 tgacaacacc acgcaagctt tgtgcggtgc tgtccaaaaa atgcgcacgg gcgaaaagtc
     4261 tgtgcgcatt tttggggtcg gaggcgcata ttttcccggc ggaaaatcag acctttcggc
     4321 ggcaaagaaa acaaatgctg gaagaatctg ttttgtgacc gtcattttgc gcaaaaatag
     4381 tgcgcaagcg gcatgaaatt cactgcacat tatggaaatt acttgacaaa gcggtgtact
     4441 ctctgttata atgaaattgt atgctgtaag cgctgtttgg aagagaaaac gggcgtacag
     4501 ctttgccggg aatgcccggc agatcctttg gaaaggaaca cacagcggca caggattctg
     4561 tgctgtgata tcatcatgaa aactaactgc aaccgagacc agttcaagcg cattttttcg
     4621 ttttttgcgg cggtgctgct ggtgggaatg tatgcagccg gctttgcatg ggtctggtat
     4681 gaaagctaca gttctgccat tctgctgccg ttttaccggc ggggcaactg ggtcatgatc
     4741 ctaatctatg tggtgctggt gctgctgttt gccaagtcat tcggcggact gaagatcggc
     4801 tatctcaagc gaacggatat gttctattcc cagacgcttt ccatcttctg tgtcaatgtg
     4861 gtgacgtatt ttcagatcag cgtggtcaac cgtgactttt ccgctacggt gccgatggtg
     4921 ctgctgacga tcgtagatct ggcggtgctg atcctgtgga tcgtgctgac caacaagctg
     4981 tatttccggc tgtatccgcc gagaaagctg gtggtcatct acggcgaccg gaacgccgcc
     5041 gagctggtgc tgaaaatgag ccgccgggtg gacaagtata tgatctgcga atccgtcagc
     5101 gcatctgcgc cgccggagga gatccgggag gcgatctccc ggtatgaggg catcatcctc
     5161 tgcgaggtct ccgggcggct gcgcaacgat ctcatcaaat attgctttgc acagaatgtc
     5221 cgggcgtata ttgcgccgaa aatatccgac attatcctaa gaggcgcaga ggagatccgg
     5281 ctgttcgaca cgccgctgct gctctgccgg aactacgggc tgacatttga gcaaaagctg
     5341 ggcaagcgta tcttcgacct ggtttttgcc gttgtctttc tggtgatcct gtcgccggtg
     5401 atgctggcgt ctgccatcgc catcaagctg gatgacggcg gaccggtgct tttcaagcaa
     5461 aagcgtctga cctatcacgg aaaggaattt tacgtctata agttccggag catggtggtg
     5521 gatgcggaaa aatccggacc gcagctggca aaggaaaacg acagccgcat caccaaggtg
     5581 ggacgcatcc tgcggaaatg ccgtctggac gagctgcccc agctcatcaa cattctccgt
     5641 ggcgatatgt ccgtggtggg acccagaccg gagcgcccag aactggcaga agaatatgaa
     5701 aaaaccatgc cggaatttga gttccggctg caagtgaagg caggactcac cgggtatgca
     5761 caggtcaccg gactgtatga caccctgcct tatgataaac tgaaaatgga cttgatgtac
     5821 attgaaaact attccatttt catggacctg cgcattattc tgatgacact gaaaactgca
     5881 ttgttccccg gggaaaccaa tgaagaggca aatgcgcacg atgcgcagga gctgctgtac
     5941 caggaggaac agatacagga gcaggaaaaa gagaaaggag atcactcatg aaagtgaccg
     6001 cagtcatcat ggcaggcggc agaggcgaac gcttctggcc caagagccgt ggcaattatc
     6061 ccaagcagtt tctctctctg accaaggacg gagaaaccat gatccagaaa accgtaaagc
     6121 gtctgctgcc tatggtggca atggaggacg tattcgtagt gaccaacgta agctatgccg
     6181 cactggtcaa ggaacagctg ccgcagctgc cggaggagaa catcctgctg gagccgtgtg
     6241 ccagaaatac agcaccctgt atcggtctgg ctgccgctgt gatccgcaag aagtacggcg
     6301 aggcaatgat gctggtgctg ccctcagacc acctcatccg ctatgaagag atgtacgtgg
     6361 acaccctgcg ccaggcagtg gctgtggcag aaaaagaaca gaatctggtg accatcggca
     6421 ttacccccac ctatccggag accggctatg gctatatcaa ctttgagcgt gacaccgacc
     6481 tgggaatgcc cggcgtttat cgggtagagc gctttgtgga aaagccggat ctggacaccg
     6541 ccaaggaata cctggcatcg caccggtatc tgtggaacag cggtatgttc gtgtggaaga
     6601 cttcttccat tctggcgaac atccagaaac tgatgccgga catctacgag ggtctggagc
     6661 gcattgaggc ggcagtcggc acaggcagct atgcgtccgt gctggaaacc gagtatcacg
     6721 cattccgcag cgagtccatc gacttcggcg tcatggaaaa ggcggagaac atttacaccc
     6781 tgccgggcag ctttggctgg gacgatgtgg gaaactggct ggcagtggag cgcatcaatc
     6841 ccaccaacga atacggcaac tatatcgagg gcgatgtcat caccatcggc acagagcgca
     6901 gcaccatctg cggcggcaag cgtctgatcg ctgcggtggg catcgaagat cttattgtgg
     6961 tagataccga tgacgccgtg ctgatctgcg ccaaggacag cacccaggat gtcaagaagg
     7021 tcatcgagaa tctgaagatc tgcaaccgaa accagctggt ataagcaaag gcataacaat
     7081 gccgcttaga tagaaaggaa aaggaaatac tatgagcaaa cacgcactga ttacaggcat
     7141 cacaggacag gacggctctt atctggcaga actgctgctg gagcagggct atgaagtata
     7201 cggcatcatg cgccggaaga gcgttgtaga ctacggcaac gtagaacaca tcaaggaaaa
     7261 gatccacttc atctatgcag acatgaccga cgtcatctct ctgatctccg caatgcgcat
     7321 ctctcaggca gacgaagtgt acaacctggc tgcacagtct ttcgtggcta ccagctggga
     7381 gcagccgctg gcaaccgcag agatcgacgc tctgggtgtt accaatatgc tggaggcaat
     7441 ccgtgccgta aagccggagt gccgtttcta ccaggcatcc acttctgaaa tgttcggttt
     7501 ggtacaggaa atgccgcaga ccgaaaagac cccgttctat ccgagaagcc cgtacggcgt
     7561 tgcaaagctg tatggtcact ggatcaccaa gaactatcgg gaaagctacg gtctgttcgc
     7621 ttgctccggc atcttgttca accatgagtc tgagcgccgt ggcctggaat tcgtaacccg
     7681 taagatcaca aacgcagttg caagaattaa actgggcgta caggatcacg tagagctggg
     7741 caacatggat tccaagcgtg actggggtca ctccaaggac tatgtccgtg caatgtggct
     7801 gatgctccag caggacaagc cggacgatta tgtaatcgca accaacgaga cccgcaccgt
     7861 tcgggaattc gtagagactg ctttcggaca cgtaggcatc aacattgagt ggcagggcag
     7921 cggcgtagac gagatcggta tcgacaaggc taccggcaag accgtggttt ccgtcaataa
     7981 gaagttcttc cgtccggcag aagtagacgt actgctgggc aatccggcaa aggcagagtc
     8041 tgtcctgggc tggaagcgtg acatttcctt ctctgaactg gtagagcgca tggtaaagaa
     8101 cgacctggct ctggtagaac acgagctgaa gtgccagaac gtataagcaa tatagcacaa
     8161 cagacaatga gatcataaaa acaggggcag agtttctctg cccttgtttc catatcatgc
     8221 acggaaggag aaacacggaa tgcgcattgc atttgatgtc ctgccgctga tcggctgccg
     8281 catgacgggc atcggctatt gtgaggcagg gcagatccag gctctcacca ggctgtatcc
     8341 ggacgatgag tttctgctgc agtttttctc ccggaaaaac gaggagatca agatccagcg
     8401 gctccagccc tatcttcggg aaaatgtaaa gccccactgg gcaaaggcat ccggctatgt
     8461 ctatcggctg gtgtccaact ttctgcccgt ctcttatcgg cactacttcg gcgataaggc
     8521 acaggtgacc catttcttca actatatcgc accgccgaag gtggcaggaa aaactgtggt
     8581 cacggtacac gatatggtgt acaagacctt tccggaaacg gtgcgtggca ggacccgtta
     8641 tatgctggac accgggctga aaaagtccat gaagcgtgcc agccgcattg tgacggactc
     8701 ggagttctcc aggcaggaga tcattcgcta tttcccgcag tttgcggaca agatccgtgt
     8761 ggtttactgc ggcgtgaata cggaaaaatt ccacccggtg gacgacccgg cgcagatcag
     8821 cgctgtcaag gagaagtacg gcatcgaccg ggactatttc ctctatctgg ggacagtgga
     8881 accccgaaag aatctggagc gtctgatcga ggcgtatgac cggttctgtc agggaaagga
     8941 gacaccgccg tatctggtgc ttgccggcgg caagggctgg ctggacagcg gcatctacaa
     9001 aaaggcagag gaacttggca ggggcgacct ggtgaagttc acggaatatg taccggatga
     9061 aatgctctgt ccgctgatgt gcggtgcgct ggcattcgtg ttcccgtcga tctatgaggg
     9121 atttgggatg ccgccgctgg aggcaatggc gtgcggcgtt ccggtgctga ccacccatgc
     9181 ggcatcgctg ccggaagtgg tcggcgagga tgctgtggtg acagacccct attcgccgga
     9241 ggacatcgcc gccggattgg agcggctgta tacggacgag gcactgcggc agcggctgaa
     9301 ggcggcaggg ccgaaacgtg cggcgcagtt cacctgggag cgctccgcag agcagcttta
     9361 tgcggtctat caggaggctt gccgtgagtg agcagagaaa gctgcatatt ttgcaggtca
     9421 acaaggcgta ttatccccac atcggcggca ttgaaagcct ggtgcggacg ttttcccggg
     9481 agctgcaaaa gcgtgcagag gtgaaagttc tggtctgcca ggaaaagggg aagggcgagg
     9541 accgggtagt cgaaggcgta ccggtacact atgcgtccag cctcggcaca tacttttcct
     9601 gtccgctgtc cttttccttt ctgcggaaat tccgggagat ggcgaagtgg gcagacgtgg
     9661 tggaattcca cgtgccgttt ccgctggcag atctggcgtg cctgctgtcc ggctatcgtg
     9721 gcggcgtggt gatcgcctgg cacagcgatg tggtgcggca aaagacactg ctccggtttt
     9781 ataagccatt gctgatgaaa ttcctgcgcc gtgcggactg catcattgcg gcgacagagg
     9841 gacacatcac cagttcgccg tttctgaccc agttccggga aaaatgccgc attatccctt
     9901 acggcattga cgtgcaggcg taccggagcg tggaacgcaa gcccatcctg cggcaggtac
     9961 agcaaaatcc ggaaacggtg cgtgtgctgt ttgtgggacg gttcgtgtac tacaagggca
    10021 ttgaggtgct gctgcacgca tttgccgggg tgcatggctg tgagctgttc ctggtgggac
    10081 acggcacgcc ggagatggaa gagaaactgc accgcatgac ggaagaggcg cacatggcgg
    10141 cgcaggtaca tttcctggga aatctgccgg aggaagatct ccgggcggcg tttgcggact
    10201 gtgatatctt tgtgttgccc tcggtggcga acagcgaggc gttcggcatc gtgcagcagg
    10261 aggcgatggt ctacggcaag ccggtcatca acacggcgct gcccaccggc gtgccgcacg
    10321 tcagcctgga cggtgtgacg ggaatcaccg tgccgccgga ggatgcagat gcactggcac
    10381 aggcgataca gaaactggca gacgacaagg cgctgcggga acagtacggc agcgcagcgg
    10441 caaagcgtgt ggcagaggaa tatgaggaga aagacgtggt agacaaggtg tatgccacgc
    10501 tgaaagaagt agcagaaagg cggcaaagga catgaaagca ctgattattg gcggcggcgg
    10561 atttgtcgga cggtatctgg cggaacacct ggtgcaggac tgcggttggg agacatttct
    10621 gacaaagctg cccaaggaga atgtggaagt agagggctgt gaggcataca atctggacat
    10681 tctggacaag gacgctgtcg agacgctgct ggtgcggctg aaaccggatg ccattttaca
    10741 tctggcggcg cagagttcag tggcttattc ctggaagaat ccccagctga cggcggatat
    10801 caacatccac ggctgtctga atgtgctgga tgcagtgcgc agcgttcccg gctatgcacc
    10861 gaaaatcttg ctgatcggtt ccggtgagga atacggcgta ctgccggaaa acaccagcct
    10921 ggtgagcgag accacgccgg tgcatccggc gaatccgtat gccatcacca agctggcgca
    10981 gaatctcttc ggcgagctgt acgccaaggc atatgatatg cacatcatga tggtgcgtgc
    11041 gttcaaccac atcggtccgg gacagctgcc ccagttcgtg gtctcggatt tctgcaaaca
    11101 ggtggcagag atc
//
LOCUS       DAWENO010000061        11062 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2494_length_11062_cov_11.808395, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000061 DAWENO010000000
VERSION     DAWENO010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_11062_cov_11.808395"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    826..835
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5948..5957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6347..6356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2418 a         3163 c         2812 g         2639 t
ORIGIN      
        1 ctcccgctct gcctccggga gcgaaccgcc gcccctgctc actgcaatct acatccgata
       61 gccgctgaag gtcggcacac gtcctgcgga ggtgtggggc tgttccagat accccagctc
      121 ctccaatact gccatatcgt tccgaacggt cgccgcagat acgtgaatat ccggcaactg
      181 tgatatggtc ttcgacccca ctggctcacc ggtccgaata tacagatcga ctaccgccga
      241 taagatcctg cgttttcgtg catccactgt aaccatcctt tctggcagat tcacttttga
      301 agtgttagca ctcaatctct ctgagtgcta atataaattt accatgattc cgatagaatg
      361 tcaagcggtt ttttgaattt tgtagagttg cacaaatacc gtgcaaataa tatgtataat
      421 ttgtgctgtt tttcatgagc gtccccgcat tttcttgatt tgtcacctaa atattttttg
      481 aaaaaggttg acataacagc gccgttgtgc tataataggg acgttgcagc gttagcacac
      541 cctcagcaga cgtgcctgcg ccctgcgaat ctcaatcaga aaggaaattt ttccatgaaa
      601 gaacaagttc agaccaatcc tgccgccgga tcagaaatga cggcgaaaaa gacccagcat
      661 cagaagatcg tcgctatggt gcagatcgcc ctgtttgcag cgatcatcgc cgtatgctcc
      721 tggatccaga ttcccatgac cgtaccgttc accatgcaga ccttggcagt attctgcgct
      781 ctggcgacac tgggcggcaa aggcggcacg atctccgtcc tcattnnnnn nnnnnctcat
      841 ttatattgta ttaggcgctg tgggcgttcc ggtgtttgcc ggatttaccg gcggcgtagg
      901 catcctgttc ggcacgaccg gcggctatat catcggcttt ttgttcaccg gactgctcta
      961 ctggctcatc acccacttcc tgggcgacaa gctgccggtc atgatcctgg cgctggtgct
     1021 gggactggcg gtgtgctatg cctttggtac ggcgtggttc atgatcgtct atgctaagaa
     1081 ctccggttct gtaggactgc tgactgcact gggctggtgc gtattcccgt tcattctgcc
     1141 ggactgcgta aagatggcgc tggcgatcct cattgcaaag cgtctcccga aacggctgat
     1201 ccagcgtgta taagctgcgt ccccaccacg ggctgtgtac cgcctttttc tgcggtcacg
     1261 gctatagtga tgccttcacc gcaaacatga cggaaaagct ggcgctcttc cgccgggatg
     1321 acccgtacat caccctgacc ggcggcacgg actgcatctg tgccagctgt ccccacagcc
     1381 tgtcccatgt gtgcgatgcg gcagaaaagg tacagcgcta tgacagcgcc gttctcacgt
     1441 tctgcggtct ctctgtgggc agtgtcctgc ggtggagcga attcactgac cgggtaagcc
     1501 ggcacatcct ggaaccggga ctgttgcagg acatctgcgg cgattgccag tggtattcta
     1561 tctgcggaaa ttttcctcag cccgaacacg aatccaaata gccaaagcgt atacacggcg
     1621 gcgtgtatgc gcttttttgt atcatgtgta cattttgtgg tgcagccctt gtgcagatag
     1681 ctgttttttg atatttatat tgatttttct cgtggatttt gttataatta aggtgagaac
     1741 cgttgctgtt ctccacagga aaatacaata tgagtctttc tccaaaggag ggaatgcgta
     1801 tgaaaaaatg gactgcactg attcttttcg gcgttctgct cacaagtgcc ggatgcggaa
     1861 acgcacctgc ggactctgcg gtgcaggaaa cgaaagccac aacagaaacc accgaagaag
     1921 tcgtctacac agaccccgtt acctacgcag tatacgaaac gctggatgcg cttactgcag
     1981 actttcccaa aaaacagccc ggcgctacac aataccagcc gccgcaggca gtcctggaca
     2041 gctgggaatt ccggcaggga atcattgata acacgtatta taactatacc ttctacgatg
     2101 ccgagcaggg acgcaccatc ttcctggaga tcggtacaac gacacactat aacaatatgc
     2161 aggaacggct ggacgatctg agccagtaca gcaccatagg acaaactatt gtacaggatg
     2221 aatactggcg catcgactac tatcccgaca atgaagatcg caccctatac ggcattatgc
     2281 ccgacagcgg cgcaggttat gcgctgatga tctggcggga tgacggcgaa aatggcaccc
     2341 aggaagaact gatttccgtc aaggatctgc tgggactctg aacgatttcg caggacagac
     2401 atttttgttt gcgttaccct tgacattttc gccgttttcc gctacaatat aagagatacc
     2461 attgctggaa acagcaagca aattcaaaag cgcctgccgc atctggaaag cggcggacag
     2521 caagcaaggg agaataccaa aaatgttgac caacgaacag aagagcatgg ataagatcgt
     2581 agacctctgt aagtcccgtg gctttgtata cccgggttct gaaatttacg gcggtctggc
     2641 taactcctgg gactacggtc ctctgggcgt agagctgaaa aacaacatca agcgtgcatg
     2701 gtggaaaaag ttcgtccagg aatccccgta taacgtggga ctggatgcct ccattatcat
     2761 gaatccggag acatgggtgg catccggtca cctgggcggt ttctccgatc cgctgatgga
     2821 ctgcaagtcc tgtaagacca gacaccgtgc cgacaacctc atcgaggact ttgacggcac
     2881 caatcctgcc ggctggacca accagcagat gatggactac atccgggaaa agcagatccc
     2941 ctgcccaaac tgcggcaaga cggacttcac cgacatccgc cagttcaatc tgatgttcaa
     3001 aacctatcag ggcgttacag aggattccaa gtcggaactg tatctgcgtc cggagacggc
     3061 acagggcatc ttcgtaaact ttgccaacat ccagcgcacc acccggaaaa agctgccctt
     3121 cggcgttgct cagatcggta agtctttccg caacgagatc acaccgggca actttatctt
     3181 ccgtgtccgt gagttcgagc agatggaact ggagttcttc tgcaagccgg acaccgacct
     3241 ggaatggttc cagtactggc gtggctactg caaggacttc ctgctgaacc tgggtctgaa
     3301 ggaagagaat ctgcgtctgc gtgaccacga ccaggaagag ctgtgcttct actccaaggc
     3361 gaccacagac ttcgagtatc gtttctcctt tggctggggc gagctgtggg gcgttgcaga
     3421 cagaaccaac tacgacctga cacagcacca ggaacacagc ggcaagtctc tggaatactt
     3481 cgacccggag accaacacca agtacatccc gtacgttatc gagccgtccc tgggcgtaga
     3541 gcgtctgttc ctgtccatcg ttaccgaggc gtatgacgaa gaagttgtgg acgctgagaa
     3601 gaacgacacc cgtgtggtaa tgcacttcca cccggctctg gcaccgtata aggctgctgt
     3661 cctgccgctg tccaagaaac tgagcgaaaa ggcacatgag gtctatgcgg ttctggcaaa
     3721 gcacttcccg gtcgactatg acgagaccgg ttctatcggc aagcgctatc gccgccagga
     3781 tgagatcggt acaccgttct gcatcacagt agacttcgag accgttgaga gcgataactg
     3841 cgttaccatc cgtggccgtg ataccatgga acaggtacgt atgcctatcg acgagctggt
     3901 cggctatctg gaaaagaaaa ttgaacttta atcgtgtatt gcacataaga taaaaaagca
     3961 cgctgttgtc tgcggagaac agcgtgcttt ttgcatagac ggcagtaatt cgtgtgaggc
     4021 gagtttttct tttagaggag ctggcaagcc agaggcttga ctgaggagtc tctggcgaac
     4081 aatgttcgcc cctacggtga gccttttgaa aaaaggggac ggggcgatgt ggcttgctat
     4141 ccttgggcaa gtctgccgtc gccatttccg ctgggcgacg ggctgggcat cgccccctac
     4201 atttctcttt tcaagggaaa caaatcgact cctctgccct gtcgggcatc tcctctaaaa
     4261 gaggagactt ttgcagcaaa cgcccagaat gccaacactg cacatacagg aaagggcaaa
     4321 caaaagcgtt tgcaagcccc aggcggacag cgaggaacga gcgattccgc cggacgggtt
     4381 gaacgagttt tccctagggc agtgttttcc ggaatagcaa aggattaccg ggcgaacaac
     4441 gttcgcccct acaaatcccc ctgtcagcta cgctgacatc ccccttttca agggggacaa
     4501 tgtgtaaagc ctcctctttt agaaaaggtg gcacgcagtg ccggtggagt cacgggcgaa
     4561 caatgttcgc ccctacgatg cgttttccaa atgatgagga ggcaacatcg aaagtcccac
     4621 cctccaacac cttgacattt ccccggaaat ctggtatgat acagaaaaac accgtttcct
     4681 ttcagaaagg atctgatctt atggcacaat atgtcaccct cacaagcgac aagcacaaat
     4741 tcaccgcact gatcctgtgc atcctgggct ttttcgggct gggcggactg caccgctttt
     4801 atgtgggaaa aatcggcaca ggactgctct ggctgctcac cggcggtctc ggcggcatcg
     4861 gcacactcgt cgacctcatc accattctgc tgggcagttt ccgggacaac acggggacgc
     4921 cgctgcggca gtgacacgcc gcctggctgc cggctggagc atcgggctgg gactgggtgc
     4981 agccgtcctg ctgtgcctgc atcagccctt gggacactgg ctggctgtgc tgccgctgcc
     5041 ccagtgccgc tttcaccagc tcaccggttt tctctgccct gcctgcggca acacccgtgc
     5101 cgtgctggcg ctgctgcagg ggcatccgct gcgctccatc ggttataatc ctatgatctt
     5161 cactctgttt gcggcgctga cctgcctgta tgcagagctg gtctgtaccg ccgtgggaaa
     5221 gccccggcgc atcctgccac gcagcaatgc cctgctgttt ctcaccgtcg gcatcgtgct
     5281 gggatacgat attgtgcgga acttctttcc ggcaatcacc ttatgcaaat aaaaagtacc
     5341 gttctcctct cggaaagcgg tacttttttc gttccggcaa agcgccagtc atatttcatg
     5401 gttatgccgt ttggagatct tcagacggtt catcgcacga ttcagcgcag cctggctgtg
     5461 ataatactgc atgatgctct gcttgtggag cagacgctcc ttggcacgct ccgcagcctc
     5521 agcggcacgc ttggcgtcga tctcttccgg cagctcgatg gcatcgccca gcaccagcac
     5581 atagtcccgg cgcacttcgg caaagccgtc gctgatggcg gcatagtgcc actgtccgtc
     5641 caccagatac ttcagttctc ccggactgac gaccgtgacc atggactcgt gtccgggcag
     5701 aatgcccatc agaccatcca ctgtcgggaa caccagctgt tctgccgagc caacataaaa
     5761 cggacggtcg gatgccagga tctccagctg aaatgtattc gccatactac atccctcacg
     5821 attccttgga ttcctgcttc atcgcctctg cctttgcgat cgcctcatca atggtgccta
     5881 cgttaaagaa cgcattttcc ggcagttcat ccattgcacc ctcgacgatc atcttaaagc
     5941 cacggatnnn nnnnnnntag tctcacgcac cggaacatat ttgcccggca aaccggtaaa
     6001 gacctctgcc acgttaaacg gctgagacag gaacttctgg atcttacggg cacgataaac
     6061 tgccagcttg tcgtcctcgt ccagttcttc catacccagg attgcgataa tgtcctgcag
     6121 ttccttatac cgctgcagca gctcctgtac cttacgggca acacgatagt gttcttcgcc
     6181 gacaacatcc ggttccagga tacgagaggt ggattccagc ggatctaccg ccggatagat
     6241 accctgctct acgatcttac gggacaatac cgtggtcgca tccaggtggg cgaacgtggt
     6301 cgccggcgcc gggtcggtca ggtcatctgc cggcacataa actgccnnnn nnnnnntccc
     6361 agcagcgccg aaacctcgga acctgcctgg acataacgga aaatgttgtc gataaacagc
     6421 agcacgtcct ggttcttctc atcacggaag tactccgcca tggtcagacc ggtctgtgcc
     6481 acacgcatac gggacccagg cggttcgttc atctgaccga acaccagggc ggttttttcg
     6541 ataacgccgg actccttcat ttcgttccac aggtcgttgc cctcacggga acgctcaccg
     6601 acaccggtga agatggaata gccgccgtgt tccgttgcga cgttgtggat cagttcctgg
     6661 atgaggacgg tcttaccaac gccggcgccg ccgaacagac cgactttacc gcccttggcg
     6721 tacggtgcca gcaggtcgat gaccttgata ccggtttcca ggatctcaac taccggactc
     6781 tggtcacgga aagacggtgc cggacggtga atcggccact tttcgtcggt cttgacttca
     6841 ccggcatcgt caatcgcctc acccagcaca ttgaacattc tgcccagggt cgctgtgcct
     6901 accggcacct taatgcagga accggtcgca aggacttcca tatcctttgc aaagccctct
     6961 gtggaggaca ggctgatgca gcggacaatg ccgttgccca tatgctgcgc aacttccatc
     7021 acgcaggttt taccgccgtt gtcgacggtg agtgcatcct tgatcatcgg aagctttccc
     7081 gggaatgcca catcaatgac aggtcctaaa acctgcacga ttttcccttt ttccatatca
     7141 tcattccttt ccggacggcg gcggcagaac tccgccgctg ccaagccttc caattcatat
     7201 tactcggtgc agcgcctcac atttctgcgg agtttgcacc gctgacgatc tctgtgatct
     7261 cctgggtgat cgctgcctgt ctcgcccggt tatactggag cgacagattt tgcagcatat
     7321 cctttgcgct ggtggtagcg gagtccattg cggacattct cgcctgctgt tcgctgcaga
     7381 acgactctac cattgcgcca tagatcatgc ccttcataaa gttcggggtc agatgttcca
     7441 taacgatgtt agggctggga gagaacacag cctcgtggtg ccgtgttatt tcaccctcgc
     7501 tctgtacgaa atgctctgca tccagcggta ggagctgtag gatcttcggt tcaaaggtaa
     7561 tggacgaaac catatcggta tagatgatat acacatcatc cagatcaccg tcatcaaacg
     7621 ctttcagcat ctgttccgca atgcggcggg aacggtagag ggtcgggttc tgggtggtat
     7681 agataaactc agtatccacg tccagttctc cggctctggc acggcgctgg aagtacattc
     7741 ttcccacctg tcccatgacg tacagatggc tctgcttgtg tgccgccatc ttctcttccg
     7801 tcagcttgat gatgttgtgg ttataggagc cgcacatacc cttgtctgct gtaatgacaa
     7861 tatagccgac ctttcgatct tcgggcttga tctcttcccg gcggtcgaaa taccgcatat
     7921 cgatgtcctc cggcgcacgg gaaagaatac tccgcatcgc atacagcagc ttattgaaat
     7981 agggctgtgt tgccgcaaga tccttctttg cctttttcag ctttgaggag gagatcagat
     8041 acatggcgtt ggtgatcttc ataatgtcgt tgatgctctt gatccggtca cggatctcac
     8101 gcatattgcc cattttagtt cacctgcttt ttataggtac gtaccagttc cagaagcttt
     8161 tctctggatt catccgaaag ctttccggtc tcgttgattt cctttacgat ggctgcgccg
     8221 tttgcctcaa cgtagttcat cagaccctca cggaacgacc gcaggtctgc cagagccaca
     8281 tcgtccagca gtccgttgga tgccgcagaa aggatcacaa cttctttcca cattgccagc
     8341 ggatgataca acggctgctt gagcagttcc atcagcacat ggccgtggtc cagcattgcc
     8401 tgtgttgccg ggtccagatc cgagctgaac tgggtgaaca cttccatttc acggaactgt
     8461 gccagatcca cacgcatctg accggacgcc tttttcatcg ccttggtctg tgcggcgccg
     8521 ccgacacggg atacggacag accgacgttg actgccggac gcataccggc gttgaacagc
     8581 tcgctctcca ggaagatctg accgtctgtg atggagataa cattggtcgg gatataagcg
     8641 gagatatcgc ctgcctgggt ctcgatgatg ggcagtgcgg tgatagaacc gccgccgtgc
     8701 gcatcgtcca gacggcagga gcgctccagc agacgggagt gcagatagaa tacgtcaccc
     8761 ggataagctt cacgcccagg cggacggtgg agcagcaggg acattgcacg gtatgccaca
     8821 gcgtgcttgg aaagatcatc gtatacgatc agcacgtcct tgccctggct catgaaatat
     8881 tctgcgattg ccgtgccggc atacggtgcg atatactgta ccggagccgg ttcgctggcg
     8941 gatgcggata ctacaatgga atagtccatt gcgccgtttt tcttcaaggt agaaacgatc
     9001 tttgccacgg tagaggactt ctgtccgatc gcaacgtaaa tacagatcac gttctgaccc
     9061 ttctggttaa tgatggtatc cactgcgatc gacgtcttac ctgtctgacg gtcgccgata
     9121 atcagctcac gctgaccacg gccgatcggg aacatcgagt cgatggaaag gatacctgtc
     9181 tgcagcggca cgttgaccga tttccggtat gcaacgcccg gtgcctcatg ctcaatgggg
     9241 aaatagtcct ctgcctccag cggatcaccg ccgtcgatcg ggttgcccag tgcgccgaca
     9301 actctgccga tcattttctg ggtcacgccg atgccggcac gcttgccggt acgtacaaca
     9361 gaagtccctt ctgtaatgcc atagtcgctg cccagcagaa cgacgccaat ggtctttgtc
     9421 tccaggttct ggacaatgcc ccgaacacct gtttcaaatt ctaccagctc gccgtacata
     9481 gcgtgctgca taccgaatac cgttgcgata ccgtcaccca cacgaacgac tgtaccggtt
     9541 tcctgcacgc cggacttccg gtcgaagtcc tcgatctcgc ttttcaagac cgatatgatc
     9601 tcacttgcat tcatggtact catgtcttac cctcacctcc gtatcaggct cttgcgcata
     9661 ttgcggatgg atgcacgcat actccgatcg tactcgtcat ccccgatatg cagcacaaag
     9721 ccgcccagca gcgccggatt ctgtttcagc tccagctgaa cctccggctc gccggttttt
     9781 gcgcagatca tctttttcat cgctgcgagc tgcgcctctg tcagcggtgt tacatattcc
     9841 agcacggcgt gcgcacaatg gcgctgctgc cgttccagtg cttgatactc tgaaaaaata
     9901 tccagtatgc tgtcgatctg accgttttgg cagacaacct tcagaaaggt gcgcagcgac
     9961 gccggaaaga tccggtcgat caccgcaaac ttttccgcct cgggaatcgt cgggttgctc
    10021 agggcatccg tcagctgggg gcagttctga aaggttgtct gtgcatccgt cacgtcggct
    10081 gacgtctttg caagcgtcag cagcacattc gcataggatg tcactgcacc attcattttt
    10141 ctgctccttc ctcagacagg aacgcattca ccagatcccg gttggtctgg tcgtccacct
    10201 tctgttccat cagcttggat gctgctgcca gcgccagatc ggcgatctcg ccctgaacgc
    10261 cgtccagtgt ctgccggcgc tcccgctcga tctccttctg tgcctctttg aggagcgtat
    10321 ctgcctgctg ctgtgccttg gctgtgatct cgctgccctg ggcgtgtgca ttctgttctg
    10381 cggtggcaat gatctgtttt gcctcctgct ttgcaccgtt cagcgtctcc tcatagtcgc
    10441 ttctgagctt ttcggcctct gccgccttct gctttgcctg gtccatatct gcctgcacca
    10501 tctgcgcacg cttttccagc atagcgccga ctctgccgaa caggaatttt ttcagaaaga
    10561 aaaacagcac gaggatattg atgacggaaa acacaatggt ccaaatatca aaatctagca
    10621 tatccactct cacagctttc tcatgaaagc tgcgctcctt tctctcagat tctgagagcc
    10681 tttccaggct ctgaaagcag acccgagggg tcttacttca taattaccat cagcagtgct
    10741 acaacgaaac cgtaaatagc ggttgcctct gccagcgcac agcccagcag cagcgccttg
    10801 ttgatcgcac cggcagcctc cggctgtctt gcaatcgcct ctgccgcctt ggatgttgcg
    10861 atgccgatac caaaaccggc tccgatgcct gtcaatactg caatacctgc tccaatcatt
    10921 ttcagttcct acctttcgat ccggctgatc cggatatgtt ttttcggacg tttcatccgt
    10981 cttatttcat aattaccatc agcagtgcaa caacgaaacc atagatagcg gttgcctctg
    11041 ccagcgcaca gcccagcagc ag
//
LOCUS       DAWENO010000062        10999 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2516_length_10999_cov_10.073830, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000062 DAWENO010000000
VERSION     DAWENO010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_10999_cov_10.073830"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2890..2899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10230..10239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2450 a         3185 c         2735 g         2609 t
ORIGIN      
        1 tgaaatcgaa acagtccgct ccgtccggaa tcgggatatg caaaatctcg ccctgctccg
       61 acaggatctt caagttccga tacaccgttg ccttgctgat gagggaattc tctgccgaga
      121 tctcccgaaa aatctcatcc gcagtggggt gattgtgcat ccggcgaacc gtctccagca
      181 ccagccgccg ctgtaatgtg ttgcgatttt tcataaaaaa tcatcctttg aagtcataac
      241 cagcgctgca tctgcgtcgc tccgccatac agctaatgat attagttctt aattaacatt
      301 atatactatc tgatttgact tgtcaagtat ttttcagaaa agattccgca ggacactgct
      361 gttattttct ccgcatctgc cagaaatata cccggacaga gtttctctct taaccgcaaa
      421 ggcagcacct gcctttcttg gaaaaaccgg tgctgccttt tctgctatgt atgctttttc
      481 tggactgcct ttatttttcg tccggagcga gggaaaccga ctcgccgttc agaatcttat
      541 tggaagtacg cattgcgcac attttgccgc acatggagca ggtgtggcga tcctgcggcg
      601 gacggctctc gaaataattc cgtgcctttt ccgggtcgat cgcctgggca aacattccct
      661 cccagtcgat tgcgcagcga gccttgctca tggcattgtc ccagtctctt gcgccgggga
      721 tgccacgtgc caggtctgcg gcatgagcgg cgatcttgga tgccacaata ccatcccgga
      781 catcctggag atccggcagg cgcagatgtt ccgccggagt aacatagcac aggaagtccg
      841 caccgctcat tgcggcgatc gcaccgccga tggcagctgt gatgtggtca tagcccggtg
      901 ccacgtctgt gaccagcggt cccagaacat agaacggtgc gccgtagcac agccgctttt
      961 gcagtttcat gttggctgcg atctcatcca tggtcatatg ccccggacct tctaccatga
     1021 cctgtacatc ccgttcccag gcacgcttgg tcagtgcgcc cagttctacc agctcactga
     1081 tctgtccggc atcggtactg tccgcattac agcccggacg cagtgcgtca cccaggctga
     1141 tggtcacgtc atactttctg agaatgtcca gcacatcatc gtagtactcg tagaacgggt
     1201 tttcgttccc ggtcatttcc atccaggcaa acaacagcga gccgccacgg gaaacaatat
     1261 tggtggtacg tttctgacgt ttcagcgcct ctactgcacg gcggttgatg ccggcatgga
     1321 tggtctggaa gtcaacgccc tctttggcgt gtgcctccag cactttcaga aaatctgttg
     1381 ccttgatgtc tgccagatct ttttccagat agccgatggc atcatacatg ggaaccgtgc
     1441 cgatcattgc cggagacatc tcgatcagct gtttccggaa ggtgtgggtc ttgccgtagt
     1501 tggacagatc catgattgcc tcgcactgga actgaatcgc cagctttacc ttttcaaact
     1561 cagcggtata gtcgtggcag tccccggaaa tgcccaggtt tacgttcatc ttcgtgcgga
     1621 gtccctcgcc gatgccgtag ggatgcaggc tcttgtggtt gatgttcgcc ggaatacaga
     1681 tcgtaccctt tgccacacgc tcccggatca gttccggtgc caggtgttct tccttggcga
     1741 ccgtctccat ttccggtgtc agaattccct gctttgcagc atccatctgt gttgcatact
     1801 ttgccatata tttttaattc ctttctgtcg gtaaaatacg ataattgtgt gcgatcggtc
     1861 cgtttccgtg tcccagagac agcccggcgg cgatcgcctg cgtgatatac gtctttgcgg
     1921 aacggacgct cttctccagc ggcattccct gcgccaaaag cgaggcaatc gccgaggaaa
     1981 gcgtacagcc cgtgccgtgg gtgttgttgg taacgatgcg ttcgccgctg atccagatca
     2041 tttcgccgtc tgcgcagagt aggtcattgc agctgcccac gctgtggccg cctttcagca
     2101 gcacggcggt gcggtatctt tctgcaagcg ttcctgccgc ctgctccatt tctgccggtg
     2161 aggtgatttc catgcccgtc agctgccgag cctccggaag attcggcgta atcacctctg
     2221 ccagcggaaa cagttcctgc gtcaaggcgg tctgcgcctg ggatctcagc agggcatcgc
     2281 cgctggtggc gaccatgacc gggtctacca ccacatgagg cagcctgtac ttccgcacag
     2341 cggctgcaat ggcgtgtatc tggggaatgt cgtacaccat gccgatcttt acggcaagcg
     2401 gcggaatatc ctcacagact gcggcgatct gctgttccag aaacgccgcc ggaacgggac
     2461 agatctcccg aacccctgtg gtgttctgcg ccgtcagcgc tgtaatggcg ctcatgccaa
     2521 aacagcccag catggtcatg gttttcaagt ctgcctggat gccggcgccg ccgctgctgt
     2581 cactcccggc aatggtcaga accggagcaa tggtcatagg cgctgctcct ttttctgaaa
     2641 cggatttgtc agttcatgca gttcccgggc tgcccgtgtc acatccggct gtccgaacac
     2701 agcggagacc acagccacgc cgtcaatgcc ggagctgtac agctgcggaa tgttgtgcgc
     2761 tgtaatgccg ccgatggcaa ccaccggaat gcagactgcc ttccggatgt cgcacagcac
     2821 ggcatagggc agcagggtgg cgtcatgctt ggtgctggtg tgaaaaacgg cgccgcagcc
     2881 cagatacgtn nnnnnnnnnt gatcgccttg tcgcccagga tctcccgtgc ctgctgcacc
     2941 ttcatgtcac gctgtcccac atggacaccg tctgcaccca gcacccttgc cgcctctaca
     3001 tcgtcatcaa tgatgcaggg aatcccggca ttgcggcaca gattcaaaat cggatacgcc
     3061 agctcgatca gccgctttgt ggtacagttc ttttcccgga tctgcagcat ggtgatgccg
     3121 ccgtcgatcg cctgctgcac ctgggtcgtc agatttgcag caggggctgt gatggcatac
     3181 agcgaataat tgatagaagt attctgcatt ttctgctcct ttcatagcat agggcattgc
     3241 cgcatcatga cgcaagtccg ttttgcggta ctgccgtaag taaaaaagaa cgcacccata
     3301 gggtacgttc tctcacaaga tacactgtgt tttcgttccc tccgttggca ttatccaaat
     3361 caggtctggt cgagatgtct gcatctcctc tcagcctgcg cacagcaagc tcccactcag
     3421 gactttctta tggcaatcct gcattgcctt atgtccttat tttcaaacag tctgttttct
     3481 gcggatatga gacagcatcc tctgcgcacc cagaccgaaa aattccacga tcgccgcata
     3541 tttgtccaca aaggtaatgg catagctttc cttatactgg ggcatccggt gcgtcaccgg
     3601 ccacatatgc ttttcaatca tgtcccgttc cagcggattc agcgcaaacc gatccaccgc
     3661 attctgcacg gcgacctccg gatggtgggc gctgtgagat ttctgaccgg gagattctgt
     3721 gcggtcatac gcatctgtct cataaaaata caggtcgtgc agcagtcctg ctcttgcagc
     3781 agaaactgca tcccaatgga aaaagcggca cagcagataa ttgtaatagg atacattcag
     3841 gctgtgctgg aatcgtgtcg tataccgatg gtgtcggaac tgtttcagcg cctgcacatc
     3901 agcgtgttcc aggatgtcgg aaaccagcgc ataatacgcc agacgtgtca gttctttctt
     3961 cgagccagtt gcatgaagca cactcggcac cgtcctttct ctattgcagc acacaggttt
     4021 tcaatttgcg atatgtttag tgtaacagat tttcggggat ttgtcaaggc ggcggcgaac
     4081 aaagccggca aacaaatttt acaggaatca gcgcaatcgc ctttcctttc aaaaaattcg
     4141 acaaaatccg tatttgtatc ttgcacaacc tttccggtca tgctataata gaaaaatggg
     4201 cagtgctgca tcgaaacgca tagaaataca gccgctgtca tatactccat tccaagaaac
     4261 aatataagat aagcgaaagg aacctgctat gtcattttgg aaaaaatcac gctatttcgc
     4321 cgtatttctc accgccgcct gtgccatgca gttctgtgcc ggctgttccg tgccgttcct
     4381 ggaaaaacag gaaagcgacg gctccggtta tctgttcacc gccagtctcc cggcaaatcc
     4441 caagagcctg gatccgcagt ctgccacaga cgccgcctcc aagacgatca tcgaaaacct
     4501 gtacgaggga ctggtcgagc tggacgaaaa cggcagtccg cagcttgccg cagcggaaag
     4561 ctacacaaca tctgcggacg gactggtcta tacctttctc ttgaaaaacg accgctattg
     4621 gttctacgac gccaaccagg acgatgtagt ggacgacgac gagacctgga aagtcacagc
     4681 atcggactac gcctatgcgt tccagcgcat ctttgatccg cagacccagt cgccctatac
     4741 caccatgttt tcctgtctgc aaaatgcaga tgccgtccag agcggtcagc tcgacccgtc
     4801 agagatcggc gtccgggcag tgagcgatac ggaattgcag ttcaccctgt cacgccctga
     4861 tgcagaattt ctcagcaatc tggcatccac cgcagcaatg ccctgcaacg aaaacttttt
     4921 ccagcagacc aaggggcgct atggactgga caaggaatca gtggcatcct gcggcgcatt
     4981 ctacctgcgg ctctggtttt acgaccagta cggcaaccag aaccagatct tcatgcggcg
     5041 caatgcagtc aatgcaaaag cccggagcgt ctatcccacc aatctgacct tccagatccg
     5101 caaaagcagc gacgaggcag ctgcggattt cacggacggc acatccgatc tgatgaccag
     5161 ctctgtctac cagccgcagt atatggaaag cgacaactat tctgttacag cgacccgttc
     5221 cgtcaccctt gggctgatct tcaacccgga tgacacggca tttgcaaacg ccaagatccg
     5281 aaaggcgctg tccatgggca tagaccgtgc caacatcggc ggcaattcca gcgatgacct
     5341 gctgccggca tacggactga ttccgccggc gatccactgg aacggcgcaa gctatcggga
     5401 cacatatccg gatgcgcaga ctgcctacga cccggacgca gcactgacgc tgttccagca
     5461 gggaatgcag gagctgggca gagaatccct ggattccgca aagatcctgg tctgcgcctc
     5521 gctgatggac tgcgacaatc tccacgatgt gatccagacc tggcaggagg tattcggttt
     5581 ctacatcggc atcgaagagg tgtccgaatc tgactactgg gatcgcctgc aaagcaaaaa
     5641 ttacactgtc gctgtctgcg gtattaccgc ctcttacgac agccctgccg gcgtactgga
     5701 acagttttca tcccgtacca acccgttcta ttacgccaat ggtaccaccg acgccgccat
     5761 ccaggcactg caccagtgcg gttcgaaaga ggaactccag caaaaatgcg cccagctgga
     5821 acaatccctt ctggacgaat gtgtatttct gcccattttc tataagaatc agtactgcat
     5881 caccaagagc aacaacaagg acatcggcta tgacccgttt tccggtgcgc tgaatttccg
     5941 gaacgcaaag cactttgaat gattccagaa gtttccttgt tcccttttcg gcgctgctgc
     6001 atacaataag aataccagca gggcctgctt tgagatagat gttccgagaa tcggtttccc
     6061 catagaaacg tttccggaac cagagggttt ccgacagaaa aggctgctgc cgaagatgag
     6121 agttttctca ctccggcagc agcctttttg tcaatcgtct ccacggtctt tttcccgcag
     6181 gcggttttcg atccgttccc ggtcacggtc ggaaagattg ccgcttttct ttattttccg
     6241 cagttctgcc tccgttttcc ggtgcagttc cttcgggtca aatttgtccg gttttcgcat
     6301 agtgcttttc cctttctcag tgccgcagtc agaacttttt ccgattctgc aagtcgtttt
     6361 cgaaccggtt cacctgatcg tgccgtccca cgactgccag aatgtccggc gcatggagct
     6421 gcatctgcgg acggatatcc accgtgactt cttccccacg ctttacggca atgatgttca
     6481 gaccataccg ctggcgcagg ttcatttcct gcaccgtcat ttccggcacg gtatccggaa
     6541 tcaaaatttc tgtgatgctg atatcatcgt tcagcgcaat gtattccatg acacgaggtg
     6601 caagcagacg gttcgccagg cgcacgccca tttcccgttc cggatagacc acctcagcgc
     6661 ccagcttatc cagcacgcag ccctgctcct ctgtcagcgc cttggcgatc acgtgcggca
     6721 cgcccagctg caaaacgatc atggtcgtca gcacgctgac ggcaaagtcg ctgccgatgc
     6781 ccacgattac agtatcacag ttctggatgc ccacattccg cagattctcc ttggaaaggt
     6841 ctgtcatcat ataagcgttg tccgtgaatg tcacggcatc gttgatgacc gtttccttcc
     6901 ggtcaatgac gatcagatct tttcctgcct cagccaggcg tttcgccagg gaaaatccaa
     6961 agcgtcccag accgatcacc gcatacagct tttctttttt cgcattgttt ctcattgttc
     7021 gctccttatc ccacggaaat ggtttcttcc ggtctcaaaa tctcactgtt ctttctgccc
     7081 tgcaccatcc agagggtagc aaccgtcagc ggtccgagcc gcccgatata catggtcaga
     7141 accagaacca gcttgctcgc agccgacagc cctgctgtca gtccgcagga aagccccacg
     7201 gtagcaaacg ccgatgtggt ctccatcaac acctggataa acgacgcatc cggctccagc
     7261 atacacagca ggaatgtgga aaccagcacc accgacaagc ccatcaaaaa gatcatcagc
     7321 gccttgtccc ggacttcctg ggaaaaccgc cggcggaaca ccgtgggatg ctgattccgg
     7381 gtataggcga tggcagacca gatgcacaca aacagcgtcg tcaccttaat accgccgcca
     7441 gtcgaacccg gcgctgcacc cacaaacatc agcagagcca tacagagcag tgatgcgttg
     7501 gaaaagtccc ccagattata gatgctgaaa ccggaggttc gggtgactgc actgtaaaag
     7561 aacgactgca tccaggtcag actgtcctgt gtcacgtgca gcagcagcgt tccggcaagc
     7621 agcagaaaca cggtcatggt cagaaccacc tttgtgtgca gggaaaagcg ccggaaccgc
     7681 cgtttctgcg caatattcaa catcaccaca aatcccagac cgccgcagat ggtcagcccg
     7741 gaagtcagca gcagcatggg gacattgttc cggtaatccg aaaggctgct gccgccgagc
     7801 agatcgaacc cggcattgtt gaacgctgaa atggaatgga acacgctgag ccagaccgac
     7861 tttcccaccg gataatcctg cagaaaaatg ggaaaggaca gcactgcgcc gatgccttca
     7921 atgctgaatg tcaccatcag catcactttc aacaaccgca ggattccctg aaagctgggc
     7981 tggttgaggg actccttaat cagtgtccgc tcccggatgc cgatcttctt gccggtcacc
     8041 aggataatgc ccacgcccag cgtcgtgata cccatgccgc ccacctggat cagcagcagc
     8101 agcaccgcct gtccgaaacc ggaaagcacg gcgttgcact ggatcgagga aagcccggtg
     8161 atgcagacgc cgctggtcgc cgtgaacagg gcgtccataa aggaaaggga ttgccccggc
     8221 tggtgagaga tcggcagcag gagcaggcct gtccctgcca ggatcaaaaa cgcaaatccg
     8281 gcggcgatca cacgccccgg tgtcaagaaa cgattccatc tcatgatatg tactccttaa
     8341 cgccggctga catatgcagg tatgccggct tttcatcaaa aaagctggca cacactgtcc
     8401 gtgtacacca gcttttctta aaacacattc cgcacggaaa ggcatccgcc tcccggaatg
     8461 caaccgcacc aaattactgt gcatcttctc cgtcacacgc tgtcgcatct gccggaaaaa
     8521 cattttcttc tgcctcatcg gaaaccggtt ccgctgtggt ctcataggca acagacgcag
     8581 actgcgccgc cttgcgggct tcccgttctg cctgctctgc ttccttcgcc gcacggcgag
     8641 cagcctctgc tgcctctgcc tcagctttcc actttccata ggatgcgttc ttatgaccgc
     8701 actccggaca gaacttctgt ccgtcgttca ccttagaacc gcattccggg caaatgctgg
     8761 cattatccag ccctgccagt tccgatttca gcacatcgat ctgtgcgatc agatccgatg
     8821 cctgctggaa cagtccctcg tactgttctt ccggctggca ctggttctcc acataatagc
     8881 gggaaccgat ctcctgataa atcgtctgga gcgaatcgtt gcagctgttg atcatgcggc
     8941 gtactcttgc acgggcagtc gcctttttgg aagtgtcggc aacttctctt gccatcttcc
     9001 ctacatcgtc gctcattttc cgaaactgat cacgaaaatc catttgttct ttctcctcca
     9061 taaaaacaca ttgccgttct ccggtactgc tattgtatga cagtcccctg tcaaatgatt
     9121 cccgaatcaa gcagttttcg cagtacagtc agagagacaa aagcgtctgt gctttctggc
     9181 aattttctga ttttatccgc agacgtatgc aaatctgggg ctgcgcagtc ccagaacatt
     9241 gctgaatgcg ttaccgttta ctgttttctg accgtcgatc gtcaccgaac ggacaaagcc
     9301 gctgtcaccc acttccagct gaatccagtt ggacggattc gaggaaagtg cgatgccata
     9361 ggcgttctgg atccgggagc gtacttcatc cacagacata taggtgacca caccataata
     9421 aggatcgtat tccgcatcgt attcgctggg gacgctgacc agatagtcat actgtctgcc
     9481 ccagatatca ccggagctgg cagttgcgcc gccgctggat gcgccgtagg ctgtcagagc
     9541 gtagtctccg tcataataca gcgcctgtcc ggctactgcg gaaaccgcat caatgacatt
     9601 ctgcggcgga ttcggtttta agatcacatt gttcacgctg ccgccgttaa acataatgta
     9661 agaataagcg gcaattgcct gggccttcat tgcctccggg tgcatggtgg aactcatctc
     9721 gttgtatacg atctgtgaaa tgatatccac atagctgccg gtcacaccgt ttgcagtgat
     9781 cgtgttggac gcactctcct gcaccagtgt ggtaccggga aaataatgaa acaaaattga
     9841 ctggtagtca tagccgccgt aggtcgcata gtagttagcg ccgttctggc tcatgcccac
     9901 acagtgtcca tatccataag tggtaaatgc aaactgaccg gcttctacag aggcagccat
     9961 agaggctgct gcgccgatgt ttgcgctgcc tgccgcgccg gaagacgcca cagctgtgga
    10021 ttctctgcgt gttgtcgcat cctgaatcgc agctgcctgt tcttccgagg gcttaacttc
    10081 ttcatactgt accaatgata agctggaatc gtaatctgaa atttcagctt tccacaaagt
    10141 atcgtcgctt tcatttgctg ctgtcgtatc agatgccgca gtactagtac ccgctgcatc
    10201 tgtttccggc actgcataag cactgactgn nnnnnnnnnt gactgcgcag gtccctgcca
    10261 gaagtgctgc aactgcaagt gcaataccca tttgtgggta acgtattttc atatcaaaac
    10321 tccattttga ccggcaaaag ccgggtagtg ataggagacc gtctgcatcg ctgcaaaccg
    10381 tcccctgccg cacagaagta ccggacgagg tctccctccc ggtcgtgtca ctcctgtgca
    10441 gcgtcagttc cgccgttatt ctggatcgtg ctgagcatgg atgcagtatc ctcaccgtca
    10501 cgcagacggc gtgccacgat caggaaacgg gagttatcct catcggaata acaggtggaa
    10561 agtgtcagaa ggctgtcgcc gtactgcacg tcaatggggt tgtcgatcat attcttggaa
    10621 ttgaccgatc ccatatagct gtcgaaggca gccttgtcgt ccagatcttc catctgccag
    10681 tacttaaagt ccgaatctgc atcgccgcct gtgatgagca atccgaccat cacataggtg
    10741 tagtctgcat aattggaact gaaagtgatg aaaggcgctt ctttatagta ggacggattc
    10801 tgccggtact gccgcagcga accgaacatg gtgttgtctg ccatgttgtg tccgtagagg
    10861 acaatatttt ctgaccatgt ctccggatca gcgccgaagt catcggtgca gtccatgaac
    10921 acggtgccgg cacggaattc ctgtccgtca aagccgtggt cgagataata ctcgttgttc
    10981 gaggtctgta ccacaggat
//
LOCUS       DAWENO010000063        10805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2568_length_10805_cov_10.970140, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000063 DAWENO010000000
VERSION     DAWENO010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_10805_cov_10.970140"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1312..1321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6899..6908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10372..10381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2370 a         2847 c         2771 g         2787 t
ORIGIN      
        1 tctgcacaca gtggtgcagc agcgcctgct ctttcaccgc agttatcagc gcttttccga
       61 cacccttgga gcgtgccgcc ggactgacga tcagttccat gatctcggca atacggtcgc
      121 agtgatgcag ctgaaactcc atgcgcaggt gcagcacacc ctgtaccttt ccctgttctt
      181 ccgctaccag caacagatgg gaggaatttg ccagtatggc atcatagccc caggtgaaga
      241 tctctttcgg aaacgctgtc tcttccagag cgcagatcag ctggtagatc tccggtaagt
      301 ctgcctgtac tgcccggcgg atcatcgccg gactccttcc acaacggcac gaacgccgct
      361 tggctctgcc agatagtccc cgatgtctac caaaacgccc tcgaacttgc cgtgaaagtc
      421 gctgccgcct gtgagaaaca ggtcgtactg cgcagcatat gcccggatga tttcccggtc
      481 tttttcgctg tttgcgtggt ggtggagttc cagtccgtcc agcccctgtt ttgccagctc
      541 cgggataatg cagaagttct gctgctgacc gctgtgcgcc agaaccgcca gaccgccggc
      601 atccttaatg gcacggagtg cctccagcgg cgaaggatag cggatgtcaa aatcacagat
      661 gccgccatgc ttgaatgttt tctggtagaa ttccccgaac atatccggcg cttttccacg
      721 ccgcaccagg tcatccatga tatgctgctt atagatatac tttccgtctg cacggcgcag
      781 ctggctcagg tcgatctcgt agccatgctg gttgaggatt tcgatctgtt tcagggaatt
      841 ggcgtggcgt gcctccaggg tcggatgcac ggcctgttcc gtgacctccg gcttttgaat
      901 gccgtatccc agcatatgta cccgaaaatc gttttcatag tcgtatgccg agatttcaat
      961 gcccgtcagt acctgcagcc cggtgtctgc cggaatctga aaacgctggg agagcgtgtc
     1021 atggtcggta atggcgatga cgtccagccc gttttgcctg gcacgggcaa ttagttctgg
     1081 aatggtaaag ctcccgtcag ataccgtgga gtgcatatga agatctgccc gtatcatgca
     1141 aacgtcccct ccgaaatgtt gaatacccgg tcacagagac cgtccataaa ttccaggtca
     1201 tggaaaatgc caatcatgct ggtgttgtcc tgcttgagtt tcagcagcat atccttaacc
     1261 aatactttgg actggttgtc cagcgaggcg gtcggctcgt ccagcagcat gnnnnnnnnn
     1321 ntttaccatg gtgtgcgcca ggttcagacg gagtcgttca ccgccggaaa aggtattggg
     1381 atagatgtcc cacagacttt ccggcaggca gaagtatgcc agcatctcct ctgccttttc
     1441 cttggcacgg ctgccgtccc atccggtttc caggagtgcc tgcatgacgt gttcctttgc
     1501 ggtagtgcgt ggcattacgc tgaggaactg ggaaacgtag ccgatctcat ggctgcgcag
     1561 gtagagaatt tcccgttcag aggcgtgcgc caggctgatc tcgccgtatg ccaggctatg
     1621 gtaaatgatg tccccttctg tgggaagata ggtgcggtgg atacacttca gaatggtcga
     1681 ttttccggcg ccggagcgtc ccacgatacc gataaactgt ccctgctcca atgcaaaatt
     1741 gatgttttca caggagtgga tctcacgctg tgcgtgatgc aggtaaaagt cttttcgaag
     1801 tcctttgatc tccagtatgt tatggaatgc cccctgtact ctactctgga tgtggtctga
     1861 ccgtctacca gaacgtgtgt taccaccggg tagccgtcca ggatgtcggc aatcaccagg
     1921 tctgcctttt tccccacttc gatggaaccg tagtccttgt cgatgcccat tgcctttgcc
     1981 ggattcagcg agaccttatt caccatgtcc ggcagcgtct gcccatattt gtcgcacatg
     2041 acaaacaggc tgtgcagcag tgcctgggga aaatagtcgg agcagagaat atccacgctt
     2101 tggttctgta tcgcctgcgc agcggacata ttgccggagt gcgaaccgcc caggagaatg
     2161 ttcggtgcgc cggcaatggt gtagaatccc atttcatgcg ccgcctttgc cacctcttca
     2221 gtaatgggaa attcactgat atccacgccc agctgacggt tcagctgtaa cttttccacg
     2281 gtatcatcgt catgcgatgc cacggtgatg tggttttgat gcgccaggtc agccagttcc
     2341 tggagctgtt ccatggaaag aatctttttg ttccggtggt gttccagcac ctcttcaaag
     2401 gttttccctt cattctccat gccgtggtat ttgtctacgg tttcccggta aatctccagg
     2461 tcacggtatt gcccctgtcc cggtgtgtga tccataaaag agatctcctg gatgagatgc
     2521 cgctgaatca tatccttggc gatctcgaag gcgtccacat tgtcgatctc cagccggagg
     2581 tgaaaccggt ggtggatgag gtggctgcgg ttctggatgt cggcaatcag ttcagccatt
     2641 tccagaacgc tttccttggt gcgcagcgga cttttcccca gcagttcatc gctgtacagg
     2701 gaaaaagagt ggtagatggt ggtgatgccc agctgcaaaa gttctttttc gcattctttc
     2761 agtgccagct caaaggaaaa tctggcggtg ggacgtggga atacaaactg ttcgattttg
     2821 tcggaatgca cgtcaatcat gcccggcaga atgtacctgc cgtgggcatt gatggtcttt
     2881 tccggtacgc tgtcacacgc accaatggct gcgatgcggt cgttgtccag ccagagtgtt
     2941 ttcccggtta tcacgccgtt cggggtgacg atttttccgt tttctattgc aatcatagtt
     3001 tctgtgtctc cttatagcag ggaatgtacc agctgctggg tgtaggcgtg ctgcggatct
     3061 tccagaatct gatcggtcag accgctttca atgatcttgc cgtccagcat aaccacggtt
     3121 cggtccgcca gcatccggat gaccgccagg tcgtgggata ccagcaggat cgagatgccg
     3181 tatctcgcct tgatctggcg gataagatcc agcacctttg cctggacgga caagtccaga
     3241 ccggtggtca cctcatccag caggagcagt gacggattat ttgccagcgc cttggaaatc
     3301 tgcacacgct gctgcatacc gccggaaaaa tttttcggtg cctctcccat acgggatgtg
     3361 agaatctcta ccgcctgcag cagttccttg gcacgctcgg tcatgtgtcc ggcgctgcgg
     3421 tttccggctg cgatcagctt ttcggcaatg ttcgatgccg cagaatactg catccgcagt
     3481 ccacgcaccg ggttctgata gaccatgccc ataatgcagt ttttgatgtg gcgctgttct
     3541 gccgcactgg caagccagat gctttctttg ccgtctttat aatcctgcag gtacgcctcg
     3601 ccggaggtgg gtgcaatgtc gaaataaagg ctctgcatta acgtggactt accggagccg
     3661 ctctctccca cgatgcccag cacctctccg ggatagacct ctacggaaac gtcattggca
     3721 gcccataccg tgccgcagac ggtacagcgg ttcttttcca gatgggtctg acactgcgga
     3781 cagcccttgc cgtagcgcac ggtcagattt ttcacctgca atactggttt ttcttcacaa
     3841 ttcacgggaa cgtacctcct tgcaatatgc agtgtcagaa cactggtaga acttttcgcc
     3901 ggtctctgca tcgtacatct catccagata cgtattggtg ctgccgcaga gacggcactt
     3961 ttttccgtgg aagttctccc gttcaaaggg aacatcgtca aacgccaggg ataccacact
     4021 agtgtgcggc ggcacggcgt aaatcttctt ctcccgtcct gcgccgaaga gatacaggca
     4081 gtcggactga tgcagttttg ggttgtcgaa tttcgggata ggactggggt tcatgatgta
     4141 acggtcctct accgtacagg gatactcggt cgggatggtc accttgccgt attttacaat
     4201 gctctcgtac agctgcagcc agatcggggc atagtccttt tcggcgtgca tcttcttggt
     4261 gacctcttct ttcggctcta cgatacggag cggttccgga atcggcactt gcaggacaag
     4321 gatctgatcg ttgcgcagcg gcacttccgg gatacggtga cgtgactgta tcaaagtcgc
     4381 tgcgtgggta tctgtcgttg tgggtacatc ggtgcagagt cccacgaatt ttttgatgtt
     4441 gaccgcattc acgctgtcat cgctgccctg gtcgatcacc ttcagggtat ctgacgggcc
     4501 gatgagggac agggtcagct ggataccgcc ggttccccag ccccgtccga tgggcagctc
     4561 ccgtgagccg aaaggcacct ggtagcccgg tatggccacc gctttcagaa tgcagcgccg
     4621 gatctcacgc tttgagcctt catccagaaa cgcataatta taatgttcag tccgttccat
     4681 gttcctgtct cctccgtgtc tttctcactg catccagctt tgattggaat gtgacataat
     4741 ggggcagctt caaatgggaa ataaatccgt tcatttccag gcagtcgccg tgcatcagta
     4801 caaattcctg atcctgtgcg gcatagtcac cgccctgctc cagttcccgg tcaatcactg
     4861 ccatagcgat cgctttgttt tcgtttctgc caaagacagc gccgtatccg ctggcaagct
     4921 ttagctgatc cttgtcgctg gcggagtgga atgtctccac ctcagtcagc aaaatctccc
     4981 ccagataaat ggattcgttt tccatgccgg gatatgcaac ctccagggaa acatagccgc
     5041 tgcggagttc tcctactgtg ggatgcacct gaccaaaacc acgcaggaca gaataggaca
     5101 agccggagag aaatccggta tctgcacggg tcatggtctg gagacgggca ctccggggcg
     5161 caggaaattc cagcaggttt tctgtgacat catacggcgg tgtgttgtcc tccggatagg
     5221 tgtccatcaa gccctctaca cgcagcatat ctgaaacacg gtgatatgct tttggcgctg
     5281 ccggtatctg ttcttccagg atgtgctgaa acaggcggtg cagttcgccg gcgtcctcat
     5341 tctcccgttc aaagtgaatg agacgatggg tgtaatcata agttgcgccc agcatttgac
     5401 cgccgggtat gtccttgaag gctgccgaaa tccggcgcac gacacgcatt tcgtcggtgg
     5461 tgacggtatt ggtgtagtgg ctgcgtttca gtgtggaacg ataggcacgc agcagaaaaa
     5521 ctgcctcttc cgcagagcct tcacactgtt tgagcgccag cgccgcatac tgctttgcat
     5581 agagtccgga ctcgctcatc accttgtcca ccaaaaggct gaacttctcg gcgattgcct
     5641 ccagttccag gtctttttcc gtgccgctgc ggtaatagtc caacagctga atgctggcgg
     5701 cgatcgcctc ttcaccgccg gaaactgcta catatgccat ttgccatctc tccttctcac
     5761 cagtctggga atgcaggtca catcgccgct gtctgttaca aaaatcaggt caatgcctgt
     5821 gggatattca tatgcctgct gatcccgcag ttccagcgca cggacaacgg tgggcgtgca
     5881 gtgcagttcc gtttcgccgt cgatgcctgg accgtaaagg gtcattgtct ccgacttttc
     5941 gccgctgtca cggatcagca gtgaggcgct ttggtgcgga tcggtcagcg tgccgcattt
     6001 cgcctgcacc agaaccgcag acagcatccg ttcatcggtc acaaaaagat agtcggcgtc
     6061 cgggacaggg acttcctggg atagtgtcac cagctggaga tcctttttca gagattcatt
     6121 ttcacaggaa gaaaaagtga cctcgttgtc cagcagggtc atgcccagcg ccagaaatgc
     6181 gccgtaatcg ccgaacagct tttctttctg cgccgccacg gagaccgtcc gggtcgggtt
     6241 ggacattgcg tccagcagca ggcggaacac agcctgtgtg tcaaaaactt cgtcaaactg
     6301 atggagtgtt ttcattgtgt cgcctccgaa tccatggaat ggaaatccac cttggttttc
     6361 tggaacatgg cattttcctg tgcaatcttt cgctgctgtg cctgttccca ttccagcagc
     6421 agatcttcct gcgtaaatac gcctttattt tctgcggcat caatgatcgc catatgcagt
     6481 gtcttttcga agtcgtcacc catggtgact gctgttccta cggtgtcccc cagagagact
     6541 actgcctcgg tgatgatcgc ctcgcccaag tagaacaggc tgttcttcac cggctcacgc
     6601 atccgtatca ttgtaaggga cttttccggt tttttgacga tggtcaccgg atactgctgc
     6661 tgtatcctct cgcccaggcg tatcacatcg ttgcgctgcg ctctggaaag aatctgaaac
     6721 agtcgtttct tttccatgct ttttcctccg tgatccagaa gtttctgaat cgttttctct
     6781 ttttatttta cttttgcttt gattttggtg gagcagaatt ccaggagaat gacggcgatc
     6841 acgaccacat aggtcagaaa gcccatttca tggaggttga agtaaaagct gcctgccann
     6901 nnnnnnnngc ctggaatacg atctgccaga accctgcacc gctggcacgc agcgcctcga
     6961 tggttccctc gtccacttct tcaatggact cggcgtagac ctttgtcaga taggcaaagc
     7021 tgtgaaaggt cagaccgatg acggctgcca cgctgcccag tccggcagat acggcaaaaa
     7081 tcagcaccca cagaacagtc ggcacagcac gcaccacagc gaccagactc tttatcagat
     7141 tggcaggtcc ggatgcggca atgtttttgg cacaaagcag tgcgcagaaa aaagccagga
     7201 cagcgccgaa aacggtagac aagataccca gacaaaatgt aaccagcagc tgatacagga
     7261 tgttctgcat ggtgtctgtg gaaagccttg gttccagaaa caccgttttc atgttgtgta
     7321 gtgtagatgc aactgcctct cccagtgcaa tttctttgta gtcgaatgac cagaacgcat
     7381 agaccgtcag caggagcagt gtcagcagca gtagtttcag aacggttgta cttttgcttt
     7441 tggcgctgat cttgatcttg ccgtttcggc ggcgttcggc gtgctgctgc aatccgcttt
     7501 gcggcgataa aactgcggca ttttccatta tagaatcacc tttctcacat gattggaaat
     7561 cagttcaatg gcgatgacca ttgcaataat cagcagaacg accaggctgg cagagctgaa
     7621 atccaaccgt ttataataca tatcaaacag ataaccgatt ccggtggcag tcagaatgcc
     7681 gatgagcgta gaactgcgga tattggtttc gatcatgtac agcacccagg tgacaatgcc
     7741 gggaataacc gatggaatca cgccctggaa tacggtctgg atgaagttcg caccggatgc
     7801 gtggagtgcc tcgatacagg aggaacttgc ctcgtcaatg gtttcgataa acgcacgggt
     7861 cagcattccg aatgtggtaa agaacagggc gaaaaatccg gtcaggatgt tctgcccaaa
     7921 ggaaaacatc agaagcattg cccagaccac atcgggaaca ttacggaaaa aggcagcaat
     7981 gattcgcacg aggcgtttca tgatgcagct gcggccggtg gactctgaac cgaacaggct
     8041 cagcgcaaat gcaaacacag cggcaattac cgttaccgcc acagaaagca gcgctgtttc
     8101 gatcagcttg tccatgattt tcggaaaccg ccgaatcacc agtgcatccg gtatcagatt
     8161 ctgaccgatc cagagaaatg ctttcgggaa tgcggcaagc ccttcctgca agtggaattg
     8221 tgtaattgct gaggcgaaga agtacacgcc gagaatgaca acgaaaacga tggtatacgt
     8281 aattttccgt ttgcggaata cgttttgtat ttgtggattc atactgcctc tccctgcata
     8341 tccagcatca gatcctgtgc attggactga tagatctcgt gaattttttc ctgtgtcagt
     8401 tccgacggcg tgccgtcaaa gaccagcttt ccggcagcaa tacccagaat tcgctgggaa
     8461 tacttcatgg caacgtccac ctggtggaga ttgacaatac aggtgatctt tctggtttgg
     8521 ttgatctgcg ccagatgatc cataatggtt ttggatgctt tgggatccag cgaggcgatg
     8581 ggttcatcac agagaatcag tttcggctcc tgcatgatgg cacgggcaat tcccacacgc
     8641 tgtttctgac cgccggaaag cgcatcgcag cgtttatacg cctgatccgc cagtcccagt
     8701 tcagagagaa tggagaacgc cttttctttt tccgattccg tgtaatgtcc gatcatgccg
     8761 ctgaaagtgg acttctgccc cagtctgccg tgcagaacat tttccataac actcagacgt
     8821 tccaccaggt tataatgctg aaaaatcatt ccggttttgg tgcgcacgtt tcgcagctgt
     8881 cccttgcgca gggacataat atcttcgccg tcatagatga tttttccctc tgttgcgtcg
     8941 atcagacggt tgatacagcg cagaatggta gattttccgg caccggatgg tccgatgatg
     9001 gagacgaact caccttcatt cacgaaaaaa ctcaaatcgt ccagcgccct ggtcgtgtta
     9061 tgatacacct tactgacgtg ctggaactct aaaattgaca tagctgattc gctcccttgg
     9121 tcaagactgc atattgcgga tcgggtcata ccaggaatct tccaccgtga caaagcccat
     9181 ctgatcggat ttcttttcca aaagtccctg ttctccggaa tctgcggaat agaaaatcag
     9241 tgaatcgttt gcgacttcat cagaagtgaa cagattctgg attgcctcga catcctccgg
     9301 gctgagggta tcgccgttat aggcaaacgg tccgttcaga accggtgtag actgaatgac
     9361 ggtgtactgt gcgccgtgaa ccgtatcgaa aggtgcgctt gcgtcatcat ttacagtata
     9421 aacgctgcct gcatctttgc ctgtaccgtc tgtgcaggtc acataaggtg caagctcaat
     9481 atcacagaaa gcggagacat ccgattttcc ggagagcaga ttgaatgcag aaccctggtg
     9541 cgagccgccg aacagaactt cggagaaaaa ggcatcgctg ccgccttcca gcaagtcgtc
     9601 tacaatcaga ttgtcgtctg caaaatggga aatgatcgta ttggtcggaa ctttgaaacc
     9661 ggaggtggaa ctattggaga cgaaagacat ttttttgccc ttgatattgt cgatggaata
     9721 gccatcgccg cttttgtact cgtcggcatt ttcggatttt acggcaaaga aactataata
     9781 gagcgcatca tccagtgtgc cggattcacc ggactgtaca aatagcggat ttacggcatc
     9841 gtttgcggtt tttgcctggc agtatccttc tgcacccatg cagcagccaa tctgtgccgt
     9901 gccgttggaa agggactcga ttgcgatagc gtagtcggtt gtcagcttct gttccacttt
     9961 tttgccggtt gccttttcga taagcttgcc aacctcatct cttgcagcct ggtaatcttc
    10021 cgcagattcg ttgggatacc aaaccagtgt gatggtatct gtggtgcttg tgcctgcgtt
    10081 tgcagctgcg ctttcgctgt ccgaagtcga agaaccgttg gaatttgtgc agccggtcgc
    10141 tgcaccgcaa atcattgtga aagccagaat tgctgcccag gtctttttta cgttcatgtg
    10201 aaaaactcct cttttcataa tcttagaaat acaaatcctc ttcgcaattt ccgtaattca
    10261 ttataccttt ctcaagtaaa gttcactagc aatgtcaagt gaaactctca taaaagataa
    10321 ttgcgaaacc gatttcctgc gttaagcttt tgagaataat tttccttatg cnnnnnnnnn
    10381 nttttcgggc agcaaaaagc agcctgtctc ttttgaaaca gactgctttt cgttttgtct
    10441 tttatttatg cctttgcaaa atatccgatg acgtcttttc gggtgataat tcccacaaat
    10501 acaccccgat cgtccaccac tggaacaaag ttttgatcca tgacacgctg gagtaagtcg
    10561 tccatggttg ctgttacacg aaccggcggg ttaaaatccg cacgaatcag atctgaaact
    10621 gcgtgatgct ccagattgcg gatatcctgt gtcagagatg atttcttcgg cacttttccg
    10681 agaatgttcc agaggaagtc accctcggta atcgttccgt ggtagattcc ttcgtggtca
    10741 atcaccggaa ttgcggtata cttatgatag aacatttttt ccagaccctg ccggacagag
    10801 tcgtc
//
LOCUS       DAWENO010000064        10739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2583_length_10739_cov_13.155185, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000064 DAWENO010000000
VERSION     DAWENO010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2583_length_10739_cov_13.155185"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2085..2094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2510 a         2702 c         2956 g         2561 t
ORIGIN      
        1 aggagttcga tagaatgaca gatgaaagca tgagaaagac catcgccggg aacatctccc
       61 ggtatcgcaa acaactgggt atgacacaac ttgatctttc cgaaaagatc aattattctg
      121 ataaaagcat ttcaaaatgg gagcgtggcg acggcattcc cgatgtgccg accatggtgc
      181 agctggcaga gatcttcggg gtatcggtgg atgatctgat ttatccgccg caggaacagc
      241 cgcagcagcc ggtagagtca gaagaaccgg aaaagcgcgc aaaaccgggg gattctactg
      301 acggtagatt ctgctggagg cactttttca tcactctact ctccattgcc ggggtgtggc
      361 tggtggcaat tctgatcgtc tgcctgttcc agtacattgc gccgggcatc gccgacctgt
      421 gggacggcag agtgatgtgc tatgcggtga cagcatcctt tgtggtgttc ctcatttttg
      481 ccagtctctg gtggccacgg gcgtggatct tcattgccat ttccggcatc gtctggggtg
      541 cggcgctgtc cgttttcgtg acattttccg ggatctccgg aggcggcgtg atcttcgccc
      601 ttgccggcgt gctggaactg ccggtgttgc tgtgctattt ctggcgctgc tctgcaaggc
      661 atcagtggct catcccgtat ccgttcccaa ggaaaaaata aaacaggtgc gaaaggcaca
      721 gtactgcaat cgtgaaatgg atttcagatg tatcgtgatg aaatcgtttc cgtattgcgg
      781 cactgtgcct ttttttgtca gagaatcgaa aattctcaaa cgggtagagt ggttctgcac
      841 gggagtaggt ggattctatc tgaaaaaagg ggtatactaa atggtgcaag aagcaaggcg
      901 aaaagcgcca aggaaagtga ggaaccccta catgaacatc acacagaaca tcggtatccg
      961 catttgcggc acaggcagca gcgttccgac acgcaccgtg accaacgacg atatggcaac
     1021 catcgtggag accagcgacg agtggatctc cacccgtacc ggcatctccg agcggcactt
     1081 tgccgaccag gagaccaacc tgtctctcgg cacagaggct gccgaaaagg cgatcgcaat
     1141 ggctggcatc cccagggaag agatcggcgt tgtgatctgc gccaccatta caccggacaa
     1201 catcgtgccg tcacaggcgt gcctgctcca gaagagcctg ggtctcacgg aacagatctt
     1261 tgcctttgac atctgcgccg cctgcaccgg ttttctgtat gccatgcaca cggcacggtc
     1321 gctgctctgc accatgccgg aaaagaaata cgccctggtt gtggcaagcg aactgctgag
     1381 ccgtgtcacc aactttgcag accggaatac ctgcgtgctg ttcggcgacg gcgcaggtgc
     1441 ggcagtcatc accctggatg aaaacagccg ctatgccttt gactgcggtg cagacggcaa
     1501 caccgaggtg ctgtactgcc cgaagtttta tcacaacacc aacccctttg ccgcatctct
     1561 gccggaacag ccggaacagc aggtgaagat ggagggcaga gccgtgtaca gctttgcagt
     1621 gcaggcactg tccggcagca ttcgccgtgt ggcggatcag gtgggcggct atccggaaca
     1681 ctacatctgt caccaggcaa acgcccggat cctccaggcg gcagccaagc agctgaaaga
     1741 acccatggaa aagtttttcc tggacattgc aaaatacggc aacacttccg cagcatccat
     1801 tgccattgcc ttggatgact gcgtgcgcag cggcgctgtc aaacgtggag aaagtgtgat
     1861 gctttccggc tttggtggcg gactcagcta cggcacagca tatttcacat tctgaggagg
     1921 cagcaccatg tatcgcagag agaccaagga aaaggcggca tatgccggca atcgtacccg
     1981 gagaactgac agcgtgaaac agcacgagat ggcggcgctc cgggagatgg aacagatggt
     2041 gctggcagcg gaaaacagcg ggggaacatc cggattggaa aaggnnnnnn nnnncgtatc
     2101 ctggacgaga accagcgtgc catggaggcg ttccggggaa agtttgagga cggctatacc
     2161 tggcgcaatg gctttctgcg gaacgtggca cggtttggcg gcagacgggc gctggcggat
     2221 ctggaaagcg gcagagtctg gagctatacc cagctgaatg cagaggcgaa ccgtttcgcc
     2281 cacggactgc ttcgtgccgg actgcggaaa ggcgatgtgg tcatggtaca gctccacaac
     2341 tgcccggagt ttgtgttcgc ctatctggcg tgccacaaga ccggcatggt gttctgtccg
     2401 gtgaacttcc ggctctctgc caaggagatc gctgactgca tggacaacag ccatccgaag
     2461 gtgttcctgt ctgagccgga agtccaagca gaagtagacc tggcgctgac cagaaccggt
     2521 tctgcgcccc agattctgct gactaccgag gaaacagcgc cgcacagcta caccggtttt
     2581 gtggcggact gtcccacaga cgacccggcg ctctcctggg aactctccat ttacgatgag
     2641 actctgcggc ttttcacctc cggcacaacg ggcagacaga agagcgtttg cctcaccagc
     2701 atcaatgagg tgctttctgc ccacgatgtg atgattcatt tccccttttc ctttgccgat
     2761 gtctccatga acaccacccc gtggtttcac cgtggcggac tccactccgg cggcattacg
     2821 cctacactgt atgccggcgg cacagtggtc atccagcgga agttccaggc ggcgcagacc
     2881 ctggatgcag tggaaaagta cgggctgacc tttatcatcg gcgttcccaa tgtgctggaa
     2941 ctgctggcgg aagagcaaca gaaccatccc agagacctga gcagcctgca cggcatcgtg
     3001 accatgggca gtccgctgga acgtgcggac tgtatccggt atcagaagat tctgacacag
     3061 cggatcttca acggctacgg cacaacggaa acgttctgga acacgttcct gcggccggac
     3121 gatctgccgg aaaaggcagg cagtgccgga cgttcctgtg tggatgatga tgtccgtgtg
     3181 atccgggtct atgacgacca caccgcatcg ccggacgatc tggcggcacg ggacggcaat
     3241 gaagtgggcg aagttatcat tcgctcggat gccaagtcgg cggcgtttta tttcggcaat
     3301 ccggaggaga cgaaacggcg gtttcacgat ggctttttct acaccaacga cctgggaaca
     3361 tgggacgaga accattttct ccggatcgtg ggcagaaagg atgatatgat tatctccggc
     3421 ggcgaaaaca tctacccggt agaggtggaa gaggtgctga acctgcaccc cggcgtggca
     3481 gactgcatcg ttacggcagc gccggatgtg aaacgtgggg agatcgtgac ggcgtatatc
     3541 atcccgtcag acccgtctct gacggcacag cagctggagc gctactgcaa ggagagtccg
     3601 ctgctcgcca actataagcg gccgcactat taccgctttg tcacagagct gccccggaat
     3661 gtcaccggaa agaaaatgca ctgtgttatg aaacagttgg cagagctgga ctgccgggaa
     3721 ggcagactga cacgggtata attgtctgct aaagctgctg tgaaaaacac ataccgcatt
     3781 ctgcaagggg atgcggtatt tttttatgaa gggattaatc cccctgtccc tgtgggacat
     3841 cccagcacaa ggcacaccct acgggcgcct tttcaagggg gacatgatga gcgacaatag
     3901 actttcgtag aaactatttt tctgtcaggt tttctttgtc acacaccatg atatctcttt
     3961 atctcgtaaa gtgatttgta taaaaatcca ggattttctg ttgcattttt tcatgaaata
     4021 tgctataata aaaattaaac agattcgcag gaaagcttgc ggaaaggagc atctatgaag
     4081 aaaattacca ttatcaccgg acattacggt tcgggaaaaa ccaacctggc ggtgaacctc
     4141 gccctacagc tggcagatgc cggagaaaag gtgacgatcg tggactttga cattgtcaac
     4201 ccctacttcc gcaccgccga ttttgcgaaa ctgtttgaag aacgtggggt gacgctggcg
     4261 gcatccatgt atgccaacac cagcctggac attccggcga tttcatttga catggagcgc
     4321 atggcgtatg agccggggta tctgatcgta gacgtgggcg gcgatgatgc cggcgccatc
     4381 ggtctgggac ggtatgcaga gggcttttcc gcctatgcgc cggatcagct ggatctgtgg
     4441 tatgtggtga accgctaccg ctatctcacg gaagaacccg gagagaccct ggaactgatg
     4501 tacgagatcg aggcggtctc ccggatgcgc cacaccggga tcgtgaacaa ctccaacctg
     4561 ggaaaagaga ccatggcaga caccgttttg caggcagtgc cgtatgcgga agagatcgca
     4621 gcgcaggcaa agctgcccct ggtctacacc acctatcggc gggatctgga cgtttcagtc
     4681 gcaaacggac tgccggtgga tgtgtttgtc aagccggtgt gggaggagtg aactgaggtc
     4741 gtcgggcgaa tgaagagtgc aatcctctct gttcgcctgc attgatatcc cggcaaaaag
     4801 cgcttatttc accgtagggg cgaacattgt tcgcccggga tattcggaaa cgcatcaaca
     4861 ggcgaacatc gctcgcccct acaataccat tttatacaat cttgatttac aacgccttat
     4921 tttataagtg ttttgtagaa taaaacgcac gcagaaagga gtgaggaata tggagaaaat
     4981 gcaggttcgt tttgaacgct gtaaagggtg cggtctctgc gtgacagtct gtcccaagca
     5041 gatcgttgcc ctgcaaaaag aacaccggaa tgaaaaggga tatttcaccg cagtatgtac
     5101 cgaccaggat gcgtgcatca gctgtgcgat gtgtgcgatg atgtgtccgg attgtgcaat
     5161 ttcaattcaa aagtgaaaga aggtcaataa ccaatggaaa gaagtctgat gaagggcaac
     5221 gaggcattgg cagaagcggc gatccgtgcc ggctgtaagt gtttctttgg ctatccgatc
     5281 acaccgcaga ccgagctgtc tgcgtatatg gcaaagcgca tggaaaaatc cggcggtgtc
     5341 tttttgcagg cagagtcgga gatcgcagcc atcaacatgg tgctgggcgc tgcatccacc
     5401 ggcgttcgtg ccatgacatc ctcctcttcg cccggcgttt cgctgaaggc agagggcatc
     5461 tcctacatgg caggctctga tctgccggga ctcatcatca acgtacagcg tggcggtccg
     5521 ggactgggtt ccatccagcc ggcgcagtct gactataatc aggcgaccca cgccatgggt
     5581 cacggcgatt ttcatattct ggtgtatgcg ccatgttccg tccaggagat ggtggacact
     5641 gtaacgctgg ctttcgacaa agcggatcag taccgtatgc cggcaatgat cctggcagac
     5701 ggtctgctgg gtcagatgat ggagccggtc acattcccgg aaccccgcac cacactgccg
     5761 gacaagtccg actgggctgc ctgcggtcac aaaaaccaac gggaacacca catcatcaac
     5821 tccctgtacc tggatgcgcc gctgctggag gcaaaggtca aggagcgttt caagcgctat
     5881 gctatcatcg aagagaccga gacagacgca gagctgtact gctgtgagga cgcagagatt
     5941 gtggttaccg catacggcgc atcggcacgt gtgtccaagt ccgcagtgac tgccgcacgt
     6001 cgcaagggta tcaaggcagg actgttccgt cccaagaccc tgtggccgtt cccgaaaaag
     6061 gaactgtgcg aggcaactaa gcacgcatcc cacatcctgg atgtggaaat gtctatgggt
     6121 cagatgatcg acgacgttcg tctggcaaca gaatgccgtc tgccggtatc gttcttcggc
     6181 agaaccggcg gcgtgatccc gactccggca gaagtattgg cggagattga aaaattggca
     6241 ggaggtgcag aataatggct gttgtattcg atcatcccaa gtccctgctg gatgtgtcca
     6301 cacacttctg cccgggctgc ggccacggta tcgtacaccg tctggtctgc gaagtcattg
     6361 atgaaatggg catcgagggc gacaccatcg gtgtagttcc ggtaggatgt tccgttatgt
     6421 cctataacta tttcggctgc gacgtcattg aggcgcccca cggcagagcg ccggcagtgg
     6481 ctaccggtgc aaagcgtgtg aacccggaca agttcgtctt ttcctatcag ggcgacggcg
     6541 acctggctgc catcggtacg gctgagaccg tccatgtcgg cacacgtggg gaaaacatcg
     6601 tggtcatctt tatcaacaac acgacctacg gcatgaccgg cggtcagatg gcgcccacca
     6661 ccctgccggg acaggtgaca cagaccacgc cgttcggcag aaacgtagag actgccggct
     6721 atcccatccg catctgcgag atgatggcga ctctcagcgg tacggcgctg gcacagcgtg
     6781 ttgccatcga cagcgtgccg cacatccgtg aggcaaagaa ggcgatccgc aaggcgttcg
     6841 agaatgaaaa ggaaaagcgt ggcttgtcca tcatcgaggt gctgtccact tgtccaacca
     6901 actggggtat gtctcccaca gagtccatgc agttcgtcaa ggacaagatg atcccttact
     6961 atccgctggg cgtatataag gatgttaccg cagaggaggg cgcaaaggca tgacaacaga
     7021 aattatttta gcaggcttcg gcggacaggg catcctgttt gccggaaaga tcctggcata
     7081 ctgcggtctg atggcaggca aggaactgag ctggctgccg tcctacggtc cggaaatgcg
     7141 tggcggcacg gcaaactgct ccgtctgcat ctcggatgaa cccatcggct ctccggtcgt
     7201 caccaccccg gactacctca tcgccatgaa cggtccgtcc tataagaagt tcattgatgc
     7261 agtcaagccg ggcggtctgg tgctgttcga caactccatc gtggaggaga cctgcgagag
     7321 aacggatatc acggcgatct cgctgccggc aacggatatg gcgacagatg ccggactgaa
     7381 cggtgcggca aacatggtgc tgctgggcag aatgctggcg gagaccagcc tgtttgacct
     7441 ggacaccgtg aaaaaggcaa tggaaaagtg cattccgccg aaacgtgcgc atctgatcga
     7501 ggcgaacctc aaggcaattc agctgggcat ggctgcaaag cagtaaggca gcccggctta
     7561 acaggataac aaaaacggct gtgagccgcc cggaaaggca gcttacagcc gtttgttttt
     7621 gagatatgga gttattttac gatggaatcc cagtctaccg ggacacctgc gcccttgctg
     7681 gcgatgtaga gcatctcgta gaagatgtcg gtggagtcca gcgtgccgtt ttcgtcgatg
     7741 tccgccgcca ggaagttttc accctccagc aggtcatcgg ttctgcccac agcctttttc
     7801 gcacaggagt acatcatctc gaaaacgtct gtggagtcga tacttccgtc ggcgttggcg
     7861 tcgcctttgg tgtaggtgat ttccttggcg ttgacggtga gggtgtagtt gatgtcagag
     7921 gaaaggtatg aacttgcaac cttaataaaa taagtgccct tttctaattt aaccgtgtct
     7981 gattttacat tggttttatc tccatttacg gattgtttca gaagagcatt ttcatcacct
     8041 gaaagaagtg agaaattcca gaatgttcta ttatcttcca aatcgacatg gttaaaagaa
     8101 attgaaactt cactagtctt atctactgta aagctgtaat aatcaacatc attattttta
     8161 tacaggtttc ctacacaggc agttgcaacg ggaattttat ttgcagtttc tattgtatca
     8221 ttggattcag cctcaaatac cccatcactg gaaaatgtat ttgaaataat ataatctaca
     8281 tcattatata aaaaggcaga cttaacaaca agatagtatg ttcccttttg caatcccatt
     8341 tttgaggttt tctgatttgt tgttgtacct gaaatttccc agtcgcaaat tttatttccc
     8401 tctgcatcag tcaggaaata attccaaaag tcacgagaat cttctatatc agtatgcttg
     8461 aaattaaatt ggatgagtcc atcctgttct aaattcagag cataatagtc aatatcttta
     8521 tcatcatgaa tgtttcctgt gatgctggaa ttcaatgcaa aggtatttgc agcacttaat
     8581 tcatcgttat gttcttcttc aaattttttc tcatcagtac tcggagtgaa atttgcttga
     8641 atagtatagg tgtcttttag agaaagagaa tcggaagtga ctttgatata gtattttcca
     8701 cttctcaatc caattgactt aaaagtgact tttaagttgt cggcactttt tttccaagaa
     8761 tcaatttgat tttcattctc atctaaaaga tatatatccc aaaagtctct gttatcttca
     8821 ttggatgagg tactaaattg aattgcaaga gaaccgggat tatcaagatt tatgctataa
     8881 taatcaacgt cattgtcatt gcggatattt ccggaatatg gagtattcac tgagatggga
     8941 tttgcacttt ctgcgtcgtt gttgaattct gcttcgtagt acgttgttgt ttctgaaact
     9001 ggtgtataag ttacatttaa tgtgtatgtt tctttggaat gaagagatgc tgttgagaca
     9061 atataataat aagttcctgc ttttattccc tcattatcgg ttcgataagc agttccttct
     9121 ccgtcaatat cccattcctg tacaagtgta ttgttttcat taacgagttt gagagtccaa
     9181 aatgaacgat aatcgtttaa tgagggtcga gtgaatgttg cggacaatgt gccatcttct
     9241 tgtaatgtaa ctttataaca gtctacatcg ttatcagaac tcaaatttcc cgttacctct
     9301 gtgttgacat caatcgcctc agcggtttcg atggtgtcgt ttttgccgga ctctccctcg
     9361 gtctctgcga atgccgggag cgctcccaga cttcctgcgc agagcatacc ggcggtcata
     9421 agcgccagca gctttttgct tgattgcttc attttacgca acccctttct tagatgatac
     9481 tcctattata gcataatttg agcggttccg caataatttg tctccgattt ttctgcattt
     9541 cactaaaaaa cggcgtattt cgtaaaaaaa tacactgttt tttttttttt ttgtcaatta
     9601 ttcacaaatg agaaacaggg cgattttgcc ccaaaagcct acttcacgat ggaatcccag
     9661 tctaccggca cgcctgcgcc cttgctggcg atatagagca tctcgtagaa aatgtcggtg
     9721 gagtccagcg tgtcgttttc gtcgatgtcc gccgccagga agtttgcgcc ctccagcagg
     9781 tcgtccgttc tgcccacagc ctttttcgca caggagtaca tcatctcgaa aacgtccgtg
     9841 gagtcgatac ttccgtcggc gttggcgtcg cctttggtat aggtcagcat ctccgcctgc
     9901 atggtcagcg tgtattccag gtcggaaaag ccgccgatgc ccttgtgccg gacttccaca
     9961 taatacgtgc cgccacgcac cagaacggtc tcgctttccg ttttggtctt gcccagcgcc
    10021 gcatccaggg tgaccgcctt gtcctcccgg agcgttccgg ttttctcgtc aaagggataa
    10081 caggtcaccg tccagggcgt gccgctgctg ctcagatcgt ccggcagggc gtgttccagg
    10141 cacagggaaa actgtgcggc gctgtccagg gtgactttgt agtaatccac gtccgattcg
    10201 tcggaaagct ttcccgaaac gggcgtgttg ggcagcatga catccgcacg ggaagtgctg
    10261 tcgttgggtt cgctctcata ggtgctgtcg gtcggggtga agtcggcggt gagggtgtac
    10321 tgttccgcac cggaccaatt cccgtttgtc atggcgtttg tcttgacctg gatgtagaat
    10381 gtgccggggt caaagccctg ctccggaatc gtcactgagg cagttccctg cgtcagttcg
    10441 gttttgccga tctgctcccg gtcgccgttg tagaggaaca gactccagcc caggtagccg
    10501 ctgctctggg tctcgtcacc gagcgtgaca gagagctttc cgggctggtc ggtggtgaag
    10561 gtgtaatagt ccgtgtcctg gtagttgtgg aaaacgcccc ggcagggtgc ggaaagggtg
    10621 agggcgttgg cgttgctgaa actgtcgttg ggttccagct cataggcgct gtcgctttcc
    10681 cactggcttt tcggggtgaa gtttgcggtg agggtatagg tgcttgcggg cggatagtc
//
LOCUS       DAWENO010000065        10565 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2655_length_10565_cov_9.186013, whole genome shotgun sequence.
ACCESSION   DAWENO010000065 DAWENO010000000
VERSION     DAWENO010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_10565_cov_9.186013"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1926..1935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3917..3926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4718..4727
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5473..5482
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7360..7369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8784..8793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2610 a         2642 c         3017 g         2236 t
ORIGIN      
        1 tttccatgta cagcagtggg gaactgccgc agaaacaccg acgctgagcg gtgtgaaaga
       61 attctccctc tacaccggac agaacggcaa tccgggtacc ggcgtatggt acttcgatga
      121 tatcggactt tacagcgaca aaacaccggt agtgaccaca gaaactaccg taaccaccag
      181 cgaaaccact gagacaacaa cgacttctgc atcggagaca agcgaatcct ccgcagaaag
      241 cacagatacc accgcatcgg aaaccagcag tgagaccact gtgacaaccg cttcggagac
      301 cacagcatct gcaacagaga cggtttcttc cgcagaaact tcttcggaga caacgtctgt
      361 cactgcatcg gaaacaacga cttcttctga agaggcaggc gctgtgctgt acggcgatgt
      421 taacctggac ggcaaggtgg atattgtgga cgcagtgctg ctgaataagg cggcagctgg
      481 ggtagttgaa ctgaatgcta ctgcaaaaat gaatgcagac tgtgatgcgg acggcacaat
      541 gagcagcaac gacgcactgg ctctgctccg gttcctggta cacatcatca acagcctgcc
      601 ggaaaaggtg taatccgatt cctatttttc taagacatcc ccttttcaaa aatttcatat
      661 cgggcggtgt gaatcatgcc tgcatcacac ttctttgcag gcgctgaccg catcgcccca
      721 ctccgaaatc tcctttcggt aactgcatcc ggcgcacagg gtgcagtttc gattttcctc
      781 tcttgaatat ctatacggtt tgtgtaggga aacagccctt ctgagacctg ccatgcggca
      841 cagtttcaga ggggcttttt tctgcaatgt tgccttgact ttaccgacaa attctactat
      901 aatagaagag gagcaagcgg aggtttctga aacaagctcc gacagcaaac tcagattgga
      961 ttgataaaat gcacacagaa tttttaatgg gaaatgcggc aattgccatg ggcgcagtcg
     1021 ccgcaggtct gaatgttgtt tcgggctatc ccggaacgcc gtccaccgaa gtgctggaga
     1081 cgaccgccag gcacaatgac ggcagcattt atgtagaatg gtctgccaac gaaaaagccg
     1141 ccatggaact ggcagccggt gcagcatact gcggcgcacg gacaatggtg accatgaaac
     1201 aggtggggtt gaacgttgcg tccgacccgc tgatgagcct tgcctacatc ggcgtaaaag
     1261 gcggcatggt agtactggtg gcggacgatc ccggcccgat ctcttctcag acagagcagg
     1321 acacccggcg gtttgccgct ttttccaagc tgccgtgttt tgacccgtcc ggggtgcagg
     1381 aggcatatga tatgatccag gaggctttcg cctattcaga aaagtatcac acgccggtac
     1441 tgtttcgccc caccaccaga gtctgtcatg ggtatgcctc gattcaggta aaggacaagg
     1501 cagattatct ggtcaaccag ccggagggat tcgtcaaaga ttcctccaag tgggtcatat
     1561 tccccagact gtcctaccag aatcacatcc gcatggaaga gcggaacgaa caattgcagg
     1621 aggtcttttc cggctacgag cgcaactgca ttcgtccggc ggcagatccc ggctgcaaaa
     1681 agggtattgc gacgcatggt atcagctatg cctataccat ggaagtactc catggcaaat
     1741 ctgcgcccgg agtgctgaaa gtggctacgc cgttcccgtt cccggaacag ctggcagtgg
     1801 aattcttaca gggactggat gaggtgcttt gcctggaaga actggatccg gtgatcgaac
     1861 agaaactggt ttatctctgc ggcaagtatc atctgcccac aaagattcgt ggcaagctga
     1921 cgcacnnnnn nnnnntgcga cagcgtggca aaagatctgg cggcattcct gggctgggag
     1981 cagccagctg ccgcagcacc ggaaacgccg ccgcagctgc cggtgcgtcc gccggttttg
     2041 tgcgccggat gtcctcaccg tgcctcgttc tttgccgtca aagaggcaat gaaagggaag
     2101 aagagcgtat tctgcggcga tatcggctgc tatacactgg gcaatgccat gccgctggat
     2161 atggtggata cctgtctttg catgggcgct ggtgtcaaca tgacccaggg catcggcaag
     2221 gtcgaaccgg acaccacctg ctttgccttt gtgggagatt ccacgttctt cgcctcggcg
     2281 atcaccggca tggtgaacgc tgtgtacaac caggcgaaca tgacgctggt ggttctggac
     2341 aattccacca cggcaatgac cggacaccag ccccatcccg gcacgggaaa gaccatgatg
     2401 ggacaggtgg tagacaaggt gagcattgta gacaccctgc acggcatcgg cgtaaagacg
     2461 gtggagaccg tggatccgct gcatctgaaa gaggcagtgg cgtgcgtcaa gcgtgtggca
     2521 tcacagcccg gcgtaaaggc aatcattttc aagtcgccct gtgcggtgct gataaagtcc
     2581 ggaaagccgg cagagattga ggagagcaag tgcatccagt gcaagaagtg tatccggaca
     2641 ctgggctgcc ccggcatcat tctgcaggac ggcaaagtcc gtatcgaaca ggcgctgtgt
     2701 accggctgcg gtctgtgtgc gcaggtctgc ccgacacaag cgataggagg tgcttgccat
     2761 gcgtaagaac atcattttat gcggcgtagg cggacagggc attgtcctta cgtccaagct
     2821 gcttgccgca tcggcaatgg cgaaaggcat tccggtgatg agcgcagaga ccatcggcat
     2881 ggcacagaaa ggcggcagcg tgttcagctt tctgcggatg ggagatgatc tctgctgtcc
     2941 catgttcccg aaagggactg ctgacctgct gatcggcttt gagccggcag aggcagtgcg
     3001 gatgctgccc tatctgaaac cggacggcac agtgattctg aacacccatc ccattcagcc
     3061 ggtaacggca acactgggcg gcggcgcata tgacgcagcg gaaatgatcg gctatgtaca
     3121 gcggaacgcc ggaaacgtga cgctgctgga cggcgatgcc gcctgccggg agatcggctc
     3181 gccgaaggtg ctgaatatgg tgatgctggg ggctgtgctg cgcagcgatg tgctgccgct
     3241 gacattggaa gaagtgacgg aaaccatgga acgtactgta aaaccgcagt tccgggaatt
     3301 gaaccggaaa gcactgcata tacaaggata agaggaaaac acaatgaaga ttaatgaaac
     3361 acagctggaa caggtcaacg accgcattca ggcactggta cgctcaaaca gcttttacgg
     3421 aaaaaagctg gaggctgccg gcatgacaga agtacataca cccgaagagt tcgccaaact
     3481 gccgttctct gagaaaaagg atttgcgtga cgcttatccg ctggggctga tggcagtgcc
     3541 ggaggaagag attgtccgga tccattcctc ttccggtact accggcacac cggtcatcat
     3601 cccgtatact gccaaggatg tagatgactg ggcgatccag ttcaaacgct gctacgagac
     3661 cgcaggcatc accaacaagg accgcatcca ggtgacaccg ggctacggtc tgtggactgc
     3721 cggcatcggt ttccagaacg gcgcagaaaa gctgggcgct atggttatcc ccatggggcc
     3781 gggcaacaca gaaaaacagc ttcagatgat gatggatatg gaaagcacag tactttgttc
     3841 cacctcttcc tatgcgctgc tgctggcaga ggagatcggc aagcgtggca tcaaggataa
     3901 gattcgtctg aaaaagnnnn nnnnnnatgg agtcagaaga tgcgtgaccg catttccggc
     3961 gaactgggca tcgaactcta cgatatctac ggactgaccg agatctacgg accgggcatc
     4021 ggcatcagct gcaaggaaaa ctgcggaatg cactattggg atgactatat ctacctggag
     4081 atcatcgacc cggtaaccgg cgaaaatctg ccggacggcg agatcggcga gatcgtcatt
     4141 accacactgg tcaaggaagg cgctccgctg attcgctatc gtacccatga cttgtcccgt
     4201 attatccccg gcacttgcgc ctgcggcagc aagttcccac gcattgatat tatcatgggc
     4261 agaaccgatg acatgatgaa gattaaaggc gtcaatgtat tcccaaacca gatcgaggag
     4321 attctgggta cattcgagga gatctccagc gagtaccaga tccgtatttc tcacctggac
     4381 ggcaaggaca ctatgcgtat ctacgtggag tccaccggtg atgtggattt cgcagacttg
     4441 agccgccgca ttgcagagca ggtcaagagc cgcatcggat ttacgcctat cgtcaaggtt
     4501 gtcgaagtag gcgtgctgcc ccgcagtacg aaaaagaccg cccgtgtaat tgatgagcgg
     4561 tttaactaaa taagcgattc cgagaaaata attatgcgca cagcggacac ggatttttcg
     4621 tgtccgtttc tcatactgca aaacatcaca atgatggaaa ggaatgtgtc tcacatgaaa
     4681 gtattgttaa tcaacggaag tccccacgaa aaaggatnnn nnnnnnnata cggcgctcca
     4741 cgagatggaa aaggtgttcc gggaaagcgg tgtggaaaca gaactggttt ccgtcggcac
     4801 caatcccatt cgtggatgta ttgcctgcgg cggatgtcgc aagaaaggcg cctgcgtctt
     4861 ttctgatctg gtcaatgaga tcgcacccaa gttcgaggcg tgtgacggtc tggttgtcgg
     4921 cagcccggtg tactacgcat cggcaaatgc cacactggtg gcgctgctgg accggctgtt
     4981 ctacagcaca ccctttgaca agaccatgaa agtcggtgcg gcagtggcgt cggcgagacg
     5041 tggcggcttg acggcgacct ttgacgaact caacaagtac ttcaccatca gcggtatgcc
     5101 ggttgcggca gggcagtact ggaacggcat ccacggcaac cgtgccgaag aggcacagca
     5161 ggacggcgag ggcttgcaga tgatgcggac actggcacgg aacatgacct ttttgatgcg
     5221 ctccatcgaa ctgggcaagg aaaaatacgg tctgccggag aaagagccac gcatcggaac
     5281 aaacttcatc cgataaggat tccccggaaa agccaatagg agaaaaatgc ggaagatgct
     5341 ttgcggcatt ttccgcattt ttttgcaaaa agcacttgac aaatgctttt agttatgcta
     5401 tactaactac tgttggaaat agcaagcgaa aaaagaacgt atttgttctt cttttttgct
     5461 gttatagtta gcnnnnnnnn nntatttcta actataaatt gatagaagag gtgaacccat
     5521 gtcagagagc gaatgcagac agttcgagaa aaagcttgcc catttcacag caccgacgct
     5581 gctgggcatc aaatgcgcca gcctggtttc gctgtcccgg gaggaatttg acattccgtc
     5641 ttatgcggcg gcatttaacg cacgggcaga gaaaggcgga ctgcaaatgc agatcatgtg
     5701 ccgctgtcag aaacggctgc tggtgctgct gtatcgcccg aaactgctgc aaaagcggct
     5761 ggaagagccg gcaatccgga agttcctgca tcgatatggg tacacagatg acatggatat
     5821 ggcagcctgt cttgtgcggc tgcaaaagcg cattgcagaa aagccggagt ttccccacga
     5881 gatcgggata ttcctggggt atccgctgga ggatgtggac ggttttatca cacaccaggg
     5941 cgaaaactgc aaattgtgcg gctgttggaa ggtgtacggc aacgtggaac aggcacgccg
     6001 gacatttgcc aactatgaaa aatgcagagt atttctctgc gcaaagctga gtaagggatg
     6061 caatattttg caggctctga aaatatcata agacctgtgc ggtgtcacag gcagactggc
     6121 aaaaaccagt gaatttttag gaggaagaaa acatgaaagc agcagtaatt tattggagcg
     6181 gcacaggcaa taccgaacag atggcacagg caatcgcaga cgcagcaggt gcagagctgt
     6241 tcgcagtatc tgatttcagc ggcagcattg cggattatga ccgcatcgca ttcggctgtt
     6301 ctgcaatggg cgacgaagtc ctggaagagg acgagtttga gccgttcttc gcatcgatcg
     6361 agggcgacct gtccggaaag accatcgcac tgtttggctc ttatggctgg ggcgacggcg
     6421 aatggatgcg caactgggaa gagcgtgcca agaacgacgg tgcaacactg gttggcggcg
     6481 aaggtctgat cgtcaacgaa acaccggatg acgaggcact ggaaaagtgt gctgctctgg
     6541 gcgctgaact ggtaaaggaa taagccccgt aaaggtaaaa tcctgataaa aaagacaaca
     6601 tcatctcctt tatatttgcg aaaaagctgt gcaggttttt ataacttgca cagctttttt
     6661 tatgttacta gagtaatcgg aatgtactcc gctcactctc caggacgccc tctttccgca
     6721 gcttgctgat ctcagctgag agaccgctgc gatccacttc cagaaagtcc gccagctcct
     6781 gccggtcaaa tgggatggtg aatgtgcggc tccggtgctg tttcgcctgc tgattcagat
     6841 aggtcagcag cttttcccgg gtggagcgcc tggaggtgat ctccagcttt tggtgaaaga
     6901 ccagattctt tcgtgcggtc attttcagca gattgaaaat cacctggtgg tgaaaatcgc
     6961 agccgttgcc gcaggcgtgc aggatgcggc tgccgtcgat cagcatgatc tcacatggtt
     7021 cagatgccac cacctggatg ggaatggcgt ctgcctctgc acagacaaag gattccccaa
     7081 agatctggga cggcgcaacg cttgccataa tgctcctgtt gccggaatag tcgatgcgcc
     7141 acagctgaac agcgccggac agcacaatgc cgaacttttc cggcttttcc ccttcggcga
     7201 tcagcgtctg tcccttgcgg tactgctgta ccgtaacatt cagacagcgc agcatggcga
     7261 gcaggtcgtt ttcggcaatt ccggagaaaa gctgacactg ccgcagcact ggaaaaaatt
     7321 tttgcatgaa gtctgcctcc ttgttgaaat ttcaacatan nnnnnnnnnt tattatacta
     7381 taatggattc agaaagtcaa gaaaaacaaa aatcaaaaat ggtataatac agggaggaat
     7441 ccatatgata agaaaaatca tccacattga ccgggacaaa tgcaacggct gcggtgcctg
     7501 tgcaaaggct tgccacgagg gtgcaatcga catcgttgac ggcaaggcag ttctggtgcg
     7561 ggagcatttc tgtgacgggc tgggcgattg cctgccggcg tgtcccatgg atgcgatttc
     7621 atttgaagag cgagaggcac cggcatatga cgaggcagcc gttcagcgtg cgcagaaact
     7681 ggcgaaagca aagcctttct ccggatgccc gggaatgcgt gccaagcaga tgcagcatag
     7741 agagccgaca cccggtgcag tctgtcccag ccagctttcc cagtggccgg tgcagatcaa
     7801 gcttgccggc gtagcagcac cgtactttga ggatgctgac attctcattg cggcagactg
     7861 taccgcatat gcctacggaa atttccatgc cgactttatc cgtggacgca ttacgctgat
     7921 cggctgccca aaactggatg cggtagatta cacagaaaag ctgacggcaa tattcgccag
     7981 ccacaacatt cgcagcgtca cggtggcacg gatggaagtg ccgtgctgcg gcggcatcga
     8041 atacgcagtt cagaatgcca ttgcggcaag cggcaaggat atttcgtgcc gggtcgcagt
     8101 gattggaaca gacggaacga ttttggaaga gagggtatca taaatggaac agaaaatgtt
     8161 ttgctatcag tgtgaacaga ccgtaggatg ttctgcctgc accggtgcag ctggtgtgtg
     8221 cggcaagaaa gcagatacgg caaagttaca ggacaagctg accggagcgc tgatcggtct
     8281 ggcaagagcg ctggacggcg atccgggact ggcaacagcg cagacgaatg aactggtgat
     8341 cgaaagcctg ttcatgacgc tgaccaatgt caacttcgat aacgattcca tccaggcgca
     8401 gatcgaccgg gtgcatcggg aacagagcgg acttgtgccg cagtgtgctg cctgcgcatc
     8461 gccgtgcggc aagaacgagg attacgacat gaaccagctc tggcaggcgg aggagaacat
     8521 tcgctcgctg aaatcgctgc tgctgttcgg actgcgtggc atggcagcgt atgcgtacca
     8581 tgccatggtg ctgggctata ccgacgaaac ggtaaacctc tttttccggg aggcgctggt
     8641 gcagattggt ctggaactga ccatggacga actgctgccg gaggtgctga aactgggaga
     8701 agtcaatctc cagtgcatgg cgctgctgga tcgtgccaat accgaaagct ttgggacacc
     8761 tgagccgaca aaggtttcca tgcnnnnnnn nnnagggcaa ctttggcacg gcatggcaga
     8821 accagcagaa ggagttccac cagattcctg cgcccattct tttcaccaca aactgcctga
     8881 tgccgccgaa acccagctat gcagaccggg tgtttaccac agaggcagtg ggatatccgg
     8941 ggatggtgca tatcggggca gacaaggatt tcacgtcgct gattgaaaaa gccctggaac
     9001 tgggcggtta tccggagacg catcagcaaa ccggcatcaa cggcggcaca gagctgacga
     9061 ccggattcgg acacggcgca gtgctttccg tggcagatca ggtggtagag gcagtcaagg
     9121 cagggcagat ccggcacttt ttcctggtgg gcggctgtga cggcgcaaag accggacgga
     9181 attattacac ggaattcgtg aaacagacgc cgccggatac agttgtactg actctggcgt
     9241 gcggcaagta tcggttcaat gacctggagc tgggaacgat cggcggactg ccccgtctga
     9301 tggatatggg gcagtgcaac gatgcctaca gtgcaatcca ggttgcagtc gcattggcga
     9361 atgcgtttgg ctgcggcgtc aatgaactgc cgctgtccta tgtgctgtcc tggtacgagc
     9421 agaaagcggt ctgcattctg ctgactctgc tgcatctggg catccggaac attcgtcttg
     9481 gtccgtctct gccggcgttc ctctcgccgg atgtgctgca atatctggtg gacaacttcg
     9541 gcattgcacc gatcaccaca ccggaacagg acttagcaga actgctggga taaacggaca
     9601 aaaaaagcaa aaagcaatcc tgcacgggaa actttccgta caggattgct ttttcgcata
     9661 tcgagaaaac gtcagcgttg gttattcgga acgcagtgcg accacagggt ctttctttgc
     9721 cgcactcttg gacgggataa atccggcaat gagtgtcagc agtacactga tggcaatgag
     9781 aatgatagcc gccggaacgg gcaggatcgc actcaagttg ttgatgccgg tgaggtggtg
     9841 taccagcagg ttgatgggga tgcacagcag atatgttacc acaatgccca gcagtccgga
     9901 ggtgaatccg ataatgatgg tctcggcgtt aaacatactg gagacatccc gcttggatgc
     9961 gccgatggca cggagaatac cgatttcctt ggtgcgctcc tggacggaga tcagcgtaat
    10021 gacgccgatc atgatggagg agacgatcag cgaaatggca acgaatgcaa tcagcacata
    10081 ggtgatggtg ttgatgatct tggtgacgga ggacatcatc agtcccacat aatccgtgta
    10141 ctggatctgg gacatctcgt ccttgccctc gttgtaatcg gaaatcgcat cttcaatgac
    10201 gtccttgtcc tcaaaggttg ccgcatacag attgatggat gccgggtcgt ccagatcgac
    10261 atcgcccagc gctttcagat tgtcgtcata ggtggagtcg gagaactgga tcatctcgtc
    10321 ataatacaaa gcgcactgtt ctgcggaaag ggtcggcagc gtctgtgtca gtgcgccgat
    10381 cagctctgcc ggctgcatgg acgccatctg ctcctgcacc tgctgtgcgt actgtgtctt
    10441 gacctgctgc tgcatcatct gggtgaaagt gtcctttatc tcatcatcgc tcatggatgc
    10501 cagataggac tggatgtcgc tttcgctcat gctcatctgc tgcgccatgc cctgggtcat
    10561 ggcag
//
LOCUS       DAWENO010000066        10508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2671_length_10508_cov_9.887305, whole genome shotgun sequence.
ACCESSION   DAWENO010000066 DAWENO010000000
VERSION     DAWENO010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_10508_cov_9.887305"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    345..354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6478..6487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7182..7191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2445 a         2960 c         2635 g         2438 t
ORIGIN      
        1 acataggaga tgatgcccat ggaaatgccc tcggagatgc tgtagaacag cggcatagcc
       61 aggatgcaca gataagccgg gattgcctcc aggaaactgt cctctgtgaa cttgatctcg
      121 gtgatgctgc tgaacatgag gaatccgacg atcaccaatg ccggagcagt tgcgaaagac
      181 gggatggcaa tgaagatcgg cgcaaagagc atggagatga ggaacagaat cgcagtcaca
      241 accgcagtca gaccggttct gccgccttct gccacgccgg cggaggattc cacaaatgtg
      301 gtggtggtgg aagtacccat cactgcaccg gcgctggttg cgatnnnnnn nnnnccgccc
      361 ttattgcaga caccgatcag cgtacccagg gtatcgaaaa tgtctacgaa caggaacgag
      421 aaaataacca caataaagtt gatgatgctt acgccgtcaa agtcaaactt gaagcactgt
      481 ccgaatgtgg aacccagtgc ggtaaagtct gtcatagaga atgccgggat caggctgttg
      541 tagcctgcct caacatctac ggtgtacagc ccggtcagct ggcagatgat gcccagcacc
      601 caggtggaaa gaatgccgat gaggatagaa ccctttaccc gcttgatgta caggaatgcg
      661 gtgaacagtg ttcccaggat cgccagcagc gcacagatgc cgccggtctg gaagttatca
      721 cggaacgaaa cgatggtcac cagcgtcgag tcactgttga caacaacgcc ggcattttgc
      781 agaccgataa aggcgatgta tacgccgatg cccaccgaca ccgccttttt cagcgacata
      841 gggataacat tgaacagcgc ctcacggaca tttgtcagcg aaaggacaat gaagatcaga
      901 ccctcgacaa acacggcaag cagtgcgacc tgccacggat agcccatgtt gccgcagacg
      961 gtataggcga aaaaggcgtt cagtcccatg ccggcggaca gggcgaaggg caggttcgcc
     1021 atcatgccca tgcacatggt tcccaggaag gatgccagac aggttgccag cagaactgcc
     1081 gtgggattca tgccggtaga actgaggata ctagggttta ccgccaggat atacgccatg
     1141 gtcatgaacg tggtaacacc ggcaatgcac tccgttttta cattggtgtt gttttctttc
     1201 aaatggaaca gtttttcaaa cattacttgg tttctccttc atcatattct gcaatatagg
     1261 gcaggttgcg gtaaatctcg ccgcagtcca gtccgtaacc gatcacaaag tgatccggaa
     1321 tggtaaacag agaaatatcg gcatccagtt ttaccagacg gcgctccggc ttgtccagca
     1381 gtgtgataac tttcagcgaa agcggatttc tcgccttcag cagcttgatg acgtcgttca
     1441 gtgtccgtcc ggtgtcgatg atatcctcgg cgatgatgac atgatagccg ctgatgtcct
     1501 gcatcagatc catagtgatc tttacgatgc cggaagagga cgtgccgttg taatagctct
     1561 tggcgcacat aaacgctgtc tcacagggaa tggtgatggc acggcacagg tctgccataa
     1621 agacgaatgc gccgttcaga atgctgacca gcagcagagg ctttccctca tactcccggc
     1681 tgatctgtgc gcccacttcc ctgactttcg cctggatctc ttcctctgtg atgaggacac
     1741 gcttgatctt ctgttcaaat tcctcggttg tcatagataa aatacgctcc ttatgtacgg
     1801 gtagctttca ttgcccgttt attttcatac attctttggt ctgccaactc atacagtgtg
     1861 gtatatgacc ggtccggcgc ctcctcggca aacgcatatc ccactgctgt tcccagctgc
     1921 cagcccctgt cctctgcctg gttgaatgat tcgatctgct cccggatatt ggaaatgatc
     1981 tgtttcacac gagtctcatc cgtattgtag atgatgccca cgaactcatc gccgccgctt
     2041 ctgccgataa agttccctgc cgggatacac cggcgcagaa ccgccgcaaa ggactcgatc
     2101 atctgattcc cggcatcgtg tccgaaattg tcgttggtct ttttcagacc gttcaagtcg
     2161 aacatcataa agcagaggtg cttatcggaa atgacgcctg tgtgaatcag ctcattgcac
     2221 ttgttgcggt tatagagacc ggttgcctta tcccggaaca gcttgtcatg tgtaccacgc
     2281 atggtgaaaa cgcccactgc gatgatgtac accagcacgc cgatcataat gaaaaacagc
     2341 gagttggacg aacgaacaag caggaagtgc aggatgtcca gcgttcccat tgccatgagc
     2401 aaaatcatgg agagcaggaa cagccggtgc cgcatatcgt ccttgtgctt aggatagatg
     2461 cagatctcaa agaccagcag gaacagcgcc tgcaccagga tgcagtaatt gtacagttcc
     2521 tgggactccc gcagcgccag cagacgcagc agatgcgccg caaatgctgc cagaacaagc
     2581 actgcgttga ccaccagcag cacgctggtt acttttcgga tcgacccggt ttttctcgcc
     2641 gacatatgga tgtacagcgc aacaaaaaag ggtgcaaaca tgatggaaaa catactcaga
     2701 taggaaaaga acaatgcgtg tcccggaatc agccaggtga taagacgggt atctgcgatc
     2761 ttccacagac cgaccagaac ggcaaccatt ccgagcggcg acagatacag agctgactgc
     2821 atcttcatgc ggtaatacac cccgatcagc aagtagaaga tgccgaagat caccaggaga
     2881 acaccgatca gcagaaacag tgcgtcgtgc cggaacaatt tccagaacag cggataccgg
     2941 acaccctcat aaaaggtaat cgttcggtct acggttgccc gatagatcgg ctgtatctca
     3001 acgacgactt ccttgccgct gtctgcacgg atcagcggca gattcaccca gtagcagcct
     3061 gtgccgctca ccggattgga cgtatttctg ctccagtcgt acacacgctt tccctggatg
     3121 tagactctcg tgttggcgtt gatgacgtag aacatgagct gttttcctgc cagctcattg
     3181 ttcagctgaa agtggaaaat acgggtcaca cccacaggcg catccgggtc gttttcctcc
     3241 accatttccg tgtagttgat ttcctggcag gtcggctgta cacggcggct gtcgatcacg
     3301 ccgccggtat ctgtacgctc cacgaacatt ccgaacaaga acagcagaca gaaaagcagc
     3361 agcactgcgt ggatcacaac gggatatttt ttatgcaatt gtttcacctc ccagaaaagg
     3421 cttttgccga ttctgtcaaa acaaaaagcg catcacgctt tttcggctgt ctttttcttc
     3481 tccgtcacct tttctgcctc catgtttttc ggcagcacca gattcagcag aatggacacc
     3541 aaaaatacaa ctgccacaca gttctgtgca aagacattct tcaccatatc cgggaagata
     3601 ttgaacaggt ctgcgcactg agtaaagccc aaaccgatgc tcagcgacag cgccgcaata
     3661 atcatgttcc gctgagaaaa gccgcatttg ctcatcatct gcaagccgct gacaacaatc
     3721 gtaccgaaca tcataatggt acagccgccc agaacggcat ccggcagtgt cgccagcaat
     3781 gcgccgaaaa tcggaaagat gccggcaaga atcatgatga ctgcaccggt ggcgatggta
     3841 aagcggttca ctacctttgt catagcgacc agtcccacgt tctggctgaa cgaagtgatg
     3901 ggcagacagc cgaacaccga ggacagagaa ctgacaaaac cgtcacaggc aatagagccg
     3961 gaggtctcct cccgtgtaac atcacgcccc agtccggagg cagccagcgc cgaagtgtca
     4021 ccgatggtct ctgtcgcaga caccaggaaa atcagcacca cggaaatgat ggcatccgca
     4081 tggaactcca tccggaacgg catcagatgc ggaacggcga tgatggaagt ctcccgcagt
     4141 gcggaaaagt ccaccttgcc catgcagatc gccagaatgt accccacgat cagaccaaac
     4201 agcacggaca gctgcttgaa ataggacttg gcgaaaatgt gaaacaggat gcagcaaagc
     4261 agcgtcactg aacccaggat ccagtttgtg gcagagccga agtcctcact gccgctgccg
     4321 ccgccaaagg aattcgcacc gactgtcagc agagaaaagc cgatggcagt caccacgctg
     4381 gcagaaacga taggggcaat gatcctgagc cagaatttcg caaacagccc caggatgccc
     4441 tcgaccagtc cgccgatgag aacggcgccc atgatcgcac cgtagccgta ctgcgctccg
     4501 ataaagcaaa gcactgacac aaaggtaaag ctgatgccca ttacaatggg cagtccggaa
     4561 cccaccttgt gaaagatggg aaacagctgg atgagtgtac cgatgccggc aatcagcatg
     4621 gcgctctgaa tcagcattgc cgtttcagaa gatgccagac cgcacgctcc gcccacaatg
     4681 aggatgggtg caatgttggc aacgaacatg gcgagaatgt gctgcaatcc aaacgggatc
     4741 gctttatgta ccggcactaa gccgttcagc tggtacagat tgctgatcga ggcatcccga
     4801 aaattcaatt ttttcatccg gcaccccgct tactgttcct gctcccggaa cacaattgtt
     4861 ccgttggatg cgtccatgct ctccacgatt gccagcgatt ccagcggata gcccagattc
     4921 cggatgatgc ggccgccctg ctgaaagccc ttttcgacag cgataccgat gccctccacg
     4981 gttgcgccag cctgcgtcac aatggagatc agtccctgaa gcgcacagcc gtttgccagg
     5041 aagtcgtcaa taatcagcac cttgtcctcc gggtgcagga acttcttgga aacgatcacc
     5101 tggttcttgc acttgtgtgt aaaggattcc acctctgcca catagaccgc accgtcaatg
     5161 ttaacgctct tggacttctt ggcgaacacc accggaacgt ggaaacagct tgccacgata
     5221 caggcgatgc cgatgccgga tgcctcaatg gtcagaatct tattgatctc cttgtctgca
     5281 aagcggcgtt tgaactcttc ccccatctgc tggaacagct gcacgtccat ctggtgattc
     5341 agaaagctgt ccactttcag aatattgccc tcttttacga tgccgtcttt tacgattctc
     5401 tcctctaaaa aattcatgtc acttttcctt cctggatgtt ttggcaatgc cgcagtcttt
     5461 gtgcggcaaa aagccggtta tatgtagtct attataccat tctctgccgg aaaagtcaat
     5521 tcaaaatctg tggaaatccg ccgaaatatg cagaagtttt cgattttttt acgttttttc
     5581 ggatttggag gaatccaggg tgtcatgccg gtatttgaga tactccagaa agcacttgac
     5641 ggcgagtgat gcgctttccc gatcccgaag tgcgagtccg atccggcggt atgcgggaac
     5701 atccagttcc cggacacaga tgcggtacgg cacacgctgt aaaatcagtt ccggcaagat
     5761 gctgacaccc agtccgcttt ccaccatgga cataatggcg tagtcgtccc acgtggtaaa
     5821 gcgcaccgac ggagaaagtc cacagcgctc gaagatctcc gccgcatcgg cacgggcatc
     5881 cttttccaac atcataaacg gcgcatcaca aagggctgcc accgggaact tttccagtcc
     5941 tgccagagga tgattttccg gcagcaccgc cataaggcga tcctcttcca gaaaaatggt
     6001 atcgaacttt cccagggtgg gcagacgcac aaagctgcag tccaccctgc cctctgtgat
     6061 ccacttttcc agttcggtat aactgcccag cagcagctcg tattcgatgt gcggatagtc
     6121 cttctgaaac tcccggatga tgttgggcag ccagtgagtg gcgatgctgg aaaatgtgcc
     6181 gatgcggatc agccccgact gcatcccgtt cagcgcattc acctgcatct gcatcttctc
     6241 atagtctgcc accagttcct tggcatacgg cagcatatgc agcccgttgg aagtcagccg
     6301 cacgccgcct tttccacgtt ccagaagtac catgttccag tccttttcca ggtcgccgat
     6361 catccggctg accccggact gggaatagtg caggatctct gccgctctgg taaaactgct
     6421 gtactccacg gtcttgacaa aggacagata cttctgcaaa ttcatatcca tgtgcatnnn
     6481 nnnnnnnccg gcagcaagaa cctgtacgca tagaaaaagt gctgaacagg ctctgaaata
     6541 ccggctctct ataaacggat ttactcgttg acatacccat ccaaaaagct gtgtgtcaca
     6601 tgatctttct ggtaggaaat gattttgttc aagttgatat tttccacgct gcggaaaata
     6661 ttcttttcga tctgcggatt ctcctccgcc agcttggcaa tcagctgttt tatcaccatg
     6721 cgcttggaaa ctgtccgttc gtccgggtcg caggtggtgc aggtgtctgt gaaatggcag
     6781 gcgcactgga taaactgcaa gtcgtgatag cggcaccagt gcttgatgtc cgcctcccgg
     6841 atcagataca tgggacgtat cagctgcatc ccctcgaagt tggcactgtg cagcttcggc
     6901 atcatggtct ccacctgtcc gccgtagatc atgctcatga ggatggtctc gatgacatca
     6961 tcgaagtggt gtcccagggc gatcttgttg cagcccagtt cctttgcctt attgtaaagg
     7021 tgtccacggc gcatccgggc gcataagtaa cagggattat tctgcacgtt atagaccgag
     7081 tcaaaaatgt tggtctcaaa aaccgtaatg ggaatttgca gcagcttggc gttggactcg
     7141 atgatctgcc ggttttcggc gctgtagccc gggtccatta cnnnnnnnnn ngcctttgca
     7201 aagcggctga aaatgacccg gtggaacttc ttgcgcagtc cctgttccac ttcggcagcc
     7261 ttttcgtttt tcgtctcccg gcgaatcttg tcctggagcc actgcacata gccacccacc
     7321 agatttaccg cctggtatcc ctcgtcccgg agttgttccg ccacgtctgc actgaggatg
     7381 ccgctgcggc agtagatgac cagcatcttg tctttgggca gttccccgtg ggatgtctcc
     7441 agctctttct gcggaatcag tactgcgccg tcaatgtgtc ccagcgcaaa tgccgtttcg
     7501 tcccgcatat caatgatctg acaggcagac ggttccatct gtgccagttc ctgtatcgta
     7561 atttcttcta ccatgcactt acctcaccaa accgctgtga taagcggcgt ttaactctgc
     7621 gatctccttt tcgccgtcga tatacggctg gaaatagctg tccaccatac cgccgcccag
     7681 attgtcacag ccattgcaga actcacaggt ctgaccgcag acctggagcg actgggtcac
     7741 gatacggatg cgctcctccc gtgtggtgtc cttgattaaa atggatttca tcgtgatatc
     7801 ctcccacaga aaaacctgct ttcagtggta ttcctttcca cgaaaacagg ttttcaaggc
     7861 agcacaatta tgccttgccg tctaactttt cgattgcctc acgcagggtg cgccatccca
     7921 gaacggcaca cttcacacgt gccggcatat gggaaatatc ctgcagcgcc gatgcctctt
     7981 ccagttcgtc tacctcggca tccgttgctg ttcccttaat cattttcagg aacagatctg
     8041 ccaggtgcag tgcctcttcc ttggtcttgc caagtaccat gccgatcata atatccgcag
     8101 atgcctgcga aatggcacag ccgtcgccgt tgaaggacgc atcctggatg acgccgtctt
     8161 tcattaccag gttcagccag atgtcgtcgc cgcagctggg gtttacgcct tccagcttga
     8221 tggtgggaca ttccaggtca tatttgaact ccggacggac gttgtgttcc gtcagaatct
     8281 cattgtaaaa gttattcatt gccaaagccc atccttcttc gaatattgga aacgctctcc
     8341 aggaaccggt cgatctcttc cttggtgtta tagaacatca gactggcacg agcggtagaa
     8401 tgtacgccca gatggtcatg gagcggctgt gcgcagtgat ggccggcacg gatgtccatg
     8461 ccgtcgctgc tcagcacttc gctgatatcg tggggatgta cctggtctac ggcaaaggaa
     8521 acgataccgg tatggttatc ctcccggtca gagcccagca cgtggatgtg cggaatcgcc
     8581 ttcatgcccc ggaacagata cgcagtcagt tcctgttcca cagcgtggag cttgtccatg
     8641 ccgtaggatt ccagatagtc aatggctgcc gccagaccga ctgcaccgcc ggcgttgacc
     8701 gtgcctgcct cgaacttatg gggcagttcc gcataaacgg catcgtaacg ggaaacggtc
     8761 tcgatcatct cgccgccggt cagaaacggc ggcatctttt ccagcagttc ctgcttgccg
     8821 tacagcacgc cgatacccat aggtgccagc atcttatgac cggagaacgc cacaaagtcc
     8881 acgtccagtt tctgcacgtc aatgggcata tgcggtgcgc tctgtgccgc atccatgaga
     8941 atggctgcgc cgcaggctct ggcacgcttg accagttccg ggatatcgtt gacacatccc
     9001 agcacgttgg aaacctgggt caccgccacc atccgggtgt gttccgaaaa cgctgcatcc
     9061 atcttttcct tggtgattgt gccgtccggc tcacactcca gaaagaccag ctttgctccg
     9121 gtggcacggg aaaccatctg ccacggcaag atgttgctgt gatgttccat gatggaaacc
     9181 aggatctcat ccccggcttt cagatttgcc atgccgtagc tgtatgccac caggttcata
     9241 ctctctgttg cgttccgggt gaagatgatc tcttccggac aggcagcatg gatgaaccgc
     9301 tgtacagttc tccgggcagc ctcatagcgt tcggtcgcct ccacgctcag cggatacagc
     9361 ccacgcagta cattggcgtt gtatttctga tagaactcct ccactgcatc cagaacgcac
     9421 tggggtttct gcgaagttgc cgcattatcg aaatagaccc ggtcggtgtt ccggagcagg
     9481 acaaaatcct gccgcacttt ttccggattc catgctgtca tcttaaatca cctcttcaat
     9541 gatctggtgc atatattctg ctgtttttgc gtcttccgta tcctggcaga gccgttccag
     9601 acgtgccttt gccacgattt tttcagcctc ttccctgctg atgccacgtg cctcgaaata
     9661 gaacagcatt ccctgttcca gttcgccgat ggatgcgccg tggctgccct tgacatcctc
     9721 ttcggtgcag aggatcagcg gaatggtctt gttgataacg tcatcgccca gcagcagtac
     9781 gttctcttct tccttgccct ctgagccggc acagccacgc ttgaggtcaa tcgtaccacg
     9841 gaacgccttt tctgcgctgt catacagcgt accctcggca cggatatcgc tctcggtatt
     9901 tttgccgatg tggttagcga tcacattcat atccagtttc tttcccggct ggaccagata
     9961 accggtcttg tgggtaaagc tgctgccgtt tcctgccagg tcggtacgga taccggagat
    10021 cacattgccc ttgctcagat acaggcggat cacctcaaac gatgcctttt cgccggtcac
    10081 acagcccacg tcattgatgc tctcttcgct ggcatctgcc agaatctgaa tcagacggac
    10141 tcttgcgccg gactctgcac gaacgactgt ccgcagtgcc gcatgggacg cattggcttt
    10201 ccagatctgc accagtgtca gatcactgcc ggactctgcc tggacgttga cgaatgcagc
    10261 acagtcgccg ccgtcctcgc tgccgatctc catatgcacg gtatcctcgg tgttttcagc
    10321 cgcagtcacc gtcatgcccc actggaacag atcgtccacg gccttgccga caccggtctc
    10381 aacagcgctg cagtcgctgc cggattccag tgcggaaaca accgccttgc ccttttccga
    10441 ttttgcctgg atagcgcagg gctttgccgg aacatagggc tctgtctggc aggcattgac
    10501 gtgcagtc
//
LOCUS       DAWENO010000067        10482 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2680_length_10482_cov_13.474154, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000067 DAWENO010000000
VERSION     DAWENO010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_10482_cov_13.474154"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7564..7573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2299 a         2981 c         2855 g         2337 t
ORIGIN      
        1 tgcgtgatgc tccaggcact gcactgcctg tacgccgcag aacacgtgaa cctctacggt
       61 ttcggcttta cgccggagca tctggaactg atccgcaaaa cagcggagga attcgccggc
      121 gaggcattcg cccagtctat cctgctctgt cccatcggtg accgctatca tttcctcagc
      181 ggctgcacct atatcatcca ggcaggattc taccagcgtg gcggtctggt ctgaccgcaa
      241 actgttcata ccgcagccct tcccattccg gaagggctgc ttttgtcttt ctcttccacg
      301 gtgtgcgtaa aaaattaacg agtgtttttt cacggagaac acttgcactc cagtgaaaga
      361 tgtgttataa taagaattgc agtgtgtgca ggactgcctg cgcacggagt atgtcttaat
      421 tccggggaac gactccccaa atttatgcaa aaggagcagt gtccgccggg atgcggaccg
      481 ggtgagaaat catgagagta gcagagatga atcgagaagc actggaagca tttgccgctg
      541 actgtaaaaa gcagtacgag gcgtttcagg cacagggtct gaaactggat atgtcccgtg
      601 gcaagccgtc gccgaaacag ctggatctga ccaacggcat caccgactgc ctggctgagg
      661 atgactataa ggcatccaac ggactggact gccgcaacta cggctgcctg gacggactgc
      721 cggaggcaaa ggaattcttc gctcccatgc tgggcgtcaa gccggaagat gtcatcgtgt
      781 gcggcaactc cagcctgaac atcatgtact gggcaatgtc cctggcaatg accaacggcg
      841 tcatgggcag caagccctgg ggcaagtatg acaaggtgaa ggtcctctgt ccggtgccgg
      901 gctatgaccg ccactttgca gtcagcgaat tcctgggcat ggagctggtg aacgttccca
      961 tgaaggaaga cggtccggat atggacatcg tggaaaagct ggtggctgag gacgacacca
     1021 tcaaggcgat ctggtgcgta cccatgtact ccaatccggg cggcgtggta tacagcgacg
     1081 aggttgtaaa gcgctttgcg gcactgaagc ccaaggcaaa ggatttccgt atcttctggg
     1141 acaacgctta ctgcgtacac cacctggtag acaatccgcc ggtacaggca aacctgctgg
     1201 aagaggcaaa gaaagccggc aacgaggata tctgctatat gttcgcctcc acctctaaga
     1261 tcacacatcc gggttcgggt attgcgatga tcgcagccag ccagaacaac ctggcatatc
     1321 tgaaaaaggc gctgggattc tccaccatcg gatacgacaa gatgaaccag ctgcgtcacc
     1381 tgcgtttctt cggcggcaag ttcgagaacc tggtagaaca catgaaaaag cacaaggcgc
     1441 tcatcgctcc gaagttccag atcgttgaaa acaccctgga aaaggaactg gctgacctgg
     1501 gcatcgccaa ctggtccaac ccccagggcg gttacttcat ctccttcaac cagaagggct
     1561 gtgcaaagcg catcgtttct ctgtgcaagg aggctggcgt agttctgacc ggcgccggcg
     1621 catcgttccc ccacggcgta gatccggagg atgaaaacat ccgtatctcc cccacattcc
     1681 ccaccgagga agaactccag atggcaatga acgtattcgt ttgcgctgca aagctggctg
     1741 ccgcagaaca ggctctggca aagtaagaca gcggaaaccc atgaaataaa acaaaaagca
     1801 ctgtgcagaa aaccggcaca gcgctttttt acagcgtaca caagcaaaaa cgcaccatgc
     1861 agaaaagccg catggtgcgt ttgttttttt attgcagaac ctgctcacgc cagagaattc
     1921 ttttgtgagg gtacggcata gagattggtc tcccatgccg gaatatacag ctgatgccgg
     1981 atatacaggt catagcccgg attcagccgc cggatgagca gcggcagggc gaagatgtcc
     2041 tcattccggt gatagagcga aaccatcagt ttcggggaaa agtgtgcgat ggtctgggat
     2101 gcgccccaga gcgcctcacg ctcacagccc tctacatcca ttttgatgat ggtcgccggt
     2161 ctgccctgga ggatgctgtc cacagagcgt gcgtcgatct cctgcccgaa ggcagagaca
     2221 gcagactgcc gtcctgcctt ggtggaaaac ggcaccgtgg tatccagaca ccatgccaca
     2281 gcgttatacg catagacata gttcatgccg tccagatact tgcacagttt cttgaagttt
     2341 ttccggtccg gttctaccgc atagatccgg atgtacttgc cacgggtgtg ctggagaacc
     2401 tcttcgatgg tgtctccgtt atacgctccc agatccacat acacctcgtt gggtgtgagc
     2461 cggatgatct tgcggtaaat ctccgttttc ggcgtggtca cgtccatcag atagcggata
     2521 ttgccgctga tgcggaagtt gatgatgttg gcatacaccc ggcgtgactc atcgtccgcc
     2581 agcatctcgt atacctcttc cagctccgca gcgtgttcca cgcagtagtc ataggtgaac
     2641 agtccgccgc ctgccaccgg aacatccggc acgatcaggg tatggcgctg tcccagctcc
     2701 acgatcttgt ccaccagaga ctggtatccg gcggcaaacg ccagaacaat gacaaaatcg
     2761 tctacctgtg cttcgatctc ggaaagcttc cgcactttat agcctgcaaa cgaatggcct
     2821 cgcacgaatt catcgcttgc aaaaatgccg gcaagcggaa tggacttttc ccgaaagaca
     2881 cgcatgatct tttctgcacc atcgcccatc ccgtagataa agatcggacg ggtctccgcc
     2941 tgcaattttt cccacgagga ctgcttttct gtgataaaag aaagcaatgt gttcaggacc
     3001 tccttttctc tttttcgcat ggtcgacaga ctgcttagtc gttacagcag tcgttatctc
     3061 cccatgcttc cggcggcata ccagccggac ccatcatatc ccgtccacgg atgccgaaac
     3121 ggtcacgctg ttcattgatg agcaccgtaa tcagatccga gacatcacgg ggattcttga
     3181 tggagtgaat gtgtttttcc ggtgtatcta cgtctttgct gaacagaatg atcgttccgc
     3241 agccgaacag ccgctgtccc agcggaagtt ccatcttctt atcgttgatc cgatagatgt
     3301 ccagttcatc ttccaccacg gtgaggagtc ccacacgggt gatgagccgg ctctccgtca
     3361 gataataccg ggtaaaggac aggggcagcc cgaacagggt acgctttttg tccgtccaga
     3421 gatactccag cccgtctttt gtttttcctt ttgccatgac tgccgccttt ccatttgatg
     3481 actgcgttga tttacggata agccgccgct ttcagaggag gcttattttt aggcaacacc
     3541 gcacaaagct tgtgcgtaag atgcgaagcc ggatttttcg acagattgca cagaaattcc
     3601 gcaggcatac tgtcgtatgt caagggattt ctgtgcagta tgacggaaaa tcagcaagca
     3661 gattgcgtac aaagccgccg ggcgggcttt gtgcggtgtt gcctttacag tacgatctcg
     3721 cccttgccgc cctgcatggt gatcttcttg tctgtgccag ccacagaaac ggtaaaggtc
     3781 ttgtctgtgt tgctgctctc gatggagatg acattgccct tgcggcttgc cttgagggtc
     3841 agcaccaggt ttgccttggt gtcatagatc gctgcctcag cggtcttgcc gtcctccagg
     3901 ctgcagatca tagcgtctgc gccgtccagg taatcatatt cgaactggtt ctcgaatgcg
     3961 ccgaaggtca cgatggagtt cggctttgcc agaaccggca tttccataaa ggtgcaggtt
     4021 acagaatgat acttgccgcc ttcataggtc tttccggtga tgatgtcata ccacttgccc
     4081 tccggcaggt agaagtttgc cacgccgtcc tcacggaata ccggagcgca cagcaggttg
     4141 tcacccagca tatactgctg atccagatag cggcaggcga tatcgtcggt gtagtccatg
     4201 accatcggac gcatcatcgg gataccggtg gtgtgggtct tgacagcctg tgcgaacaga
     4261 tacggcatca gcttgccctt gagcttggtg aagaaccgca gcacatcgca ggactcctcg
     4321 tcgaagttcc acggcacacg gtaagaagtc gaaccgtgca gacggctgtg ggtggacatc
     4381 agaccgaatg cacaccagcg cttgtaaacg tccggagatg cctttgcctc gaatccgccg
     4441 atatcgtggc tgaagaaacc gaaaccggag gagcagagcg acagaccgcc acggagtgtc
     4501 tctgccatgg aggtgtactc tgcggagcag tcaccgcccc agtgtaccgg gaacttctga
     4561 ccgccgacag tggcgcttct tgcgaacagg cacgcctgtc ccttgccgta gtattcttcc
     4621 agcacctcaa atactgtctt gttgtacaga taggtgtagt agttgtgcat atggatcgga
     4681 tctgcgccgt tgtagtatac tgcctttgtg gggatacgct cgccgaagtc ggtcttgatg
     4741 gcagtaacgc ccatctcaca cagcttgcac acgccggcag caaaccactt tgcagcctcc
     4801 ggattggtga agtctacgat cgccatgccc ggctgccaca ggtcggtctg gaatacgctg
     4861 ccgtccgggt tcttgatgaa gtagcccttt tccatgccga tatcgaacag cttggacttc
     4921 tgggagatat acgggttgat ccagcagcac agacccagac cacggcttgc cagacgcttg
     4981 agcattgccg gcggatccgg gaactgatcc agatcccact cgaagtcagt ccactggtat
     5041 tccttcatcc agaagcagtc gaagtggaac atctggagcg gaatatcccg ttctgccatg
     5101 ccgtcgatga aggaggtaac tgtctcttca tcataggtgg tggtgaacga ggtggacagc
     5161 cacaaaccga aagtctttgc cggcggcagt gccggcttgc cggtcagatc ggtgtagttc
     5221 accagaacct cttccagatt ctcaccgccg atgaagaagt agtccagctc ttcgcccggt
     5281 acggtaaagg agaccttgtt gatggtatca gatgccactt caaaggagac cttgtctgcg
     5341 gtgttgacca gcacgccgta cttgttggag gtgatataga acgggatgca cttatagctc
     5401 tgctcggagc aggtgccgcc gtcagagttc cagttctcca cgttctgacc gttcttcacg
     5461 aaggtggtga acttttcgcc gaaaccatag acgcattcgc ctacatccag ggtcagctgc
     5521 tcccggataa aggtgttgtg tgcgtcctgg ggatagttga agaaatcgtc atcggtctgg
     5581 acagccaggc gtgcgtcacg gtggaacttg ttctcctgga tataggaggt agagcgccag
     5641 ccgccgccgg tgagccgctt tccgtcacgg gagaacgaca cgtcccagcc gtctgtgccc
     5701 ttctttacct tgactgtggt atgaccggca gtcatggaaa caaagccctc ttcctcgtgg
     5761 atctcatccg gcacatagcc ctccggcaga ttcaggtcaa actggggctt gttcttcagg
     5821 ctgcccttgt ggtggcagat atgcacacgg atcacgtccg gtgcaacggc ggtataggtg
     5881 atctcaaagg tcacgccgcc cagtgtcatc gcacggttcc agatcgcact ggatgttgca
     5941 taaattttga tggtgttctt tgtggttgtg acatcatacg cctgaacagc ataggaaacg
     6001 tcatagcctc tctggttgag ccagagtccg tcagaaaatt tcatagagta cctcctgcgt
     6061 ttcaaaatag tatctgttgt attctatgcg actttccggt acaccggagt gtttgcctgt
     6121 gcccgtaccc atctacatat gctttcattg taccatactt gtcatggaaa tgtcaatctg
     6181 ttttcgtgaa aatcgtatat ttttcttgaa aatcgtatat ttttccctgt cggagtgacc
     6241 atatgccaag tttttctttc gcccagaaaa aggaaaactg ctcgcctcac agagggaaac
     6301 gattcttcaa aacttttctg ccgcccaggt gaacagcttt accaccagcg gcatggtcag
     6361 catggagacc agagtggtga cggcgaaaat gtgggaggca taggcgtcgt tcatgctgtg
     6421 cttctggcac tggagcgtac acattgccgc agagggcgca gccatcagca gcagtaccac
     6481 caggcggatc tcctggggaa taaagcccag cggcaggaac agcagcccca ggagcagcgg
     6541 cacgatcagc agctttacgg cgctgacgta gtacacccgg ggatttttca gcgcatggaa
     6601 caggtccgcc tgcgccacgc tgatgccgga cgccaccatc gccataggcg tgttcatgct
     6661 tgccacatag ccgaaggtct tttccagcag atcggggagc ggaatgcgga agaagaacag
     6721 gagcagtccg atgacaatgc cgatcatggt gggcgaacag agctttttca gcagagcctt
     6781 ccggtcgtgc tgcccggtca gcagcatgat gccatgcgtc caggcaaagc agaagaacac
     6841 gctcaggaat gccgtggtat agaacacacc ctcattcccc agaatggcgc tggcgagcgg
     6901 aatgcccata aagccgcagt tgctgtagat ggcggaaaaa cgctcgatct ccgtctcacg
     6961 ccccggcttc ggacggatca gcagatacac cgccgcaatg aatatcccat acgccgccgc
     7021 agccagcagg aacgtccaca gcatattcac caccagccgg gactcatact ccacatcggc
     7081 ataggcgttc accaccagca ccggcgtcac gatctccagc acgaagttgg agaactgtct
     7141 gccgccgttt ttatcgacga cctttgtccg ataggcgatg atgcccacca cattcagcag
     7201 cagcatcagt atcgcctgtt ttaatatgat ccatgccaaa acagaacacc tctatcccaa
     7261 aaatcggcag agtccggaca agccagaccc tatcgatcag tatatcacat aaagttttct
     7321 gttttgtgtc ctgcgtgtgg cgactgccgg aattttcggg aaaagcgccg gatctctgca
     7381 aaaaacctgc tgtctgaccc ggttttcatc caaaaatggg ctgaatctgt ttgccctccc
     7441 gtgccagcgc caccgtctcc ggcagccggg agaagtctgc caccagggaa gtgggctttt
     7501 cccggggcgc atcctggcgc aggggaacga agtaatagtg ccgggtattc agcagcagcc
     7561 cgannnnnnn nnntttcgcc gatgcgccca gggcatcgtt ggttgccacg gcgagaacca
     7621 ccggtttttg ccgccggagc atggatttca ccgccatggt cgccgtattg tcggtgatgc
     7681 cgtaggcaag cttggcggcg gtgttggcgg tacacggcgc aaccaccagc agatccagca
     7741 tatttttcgg tcccagcggt tcggcgtcgg ggatggtgcg gatggctttt ctgccgcaga
     7801 tctcttccag ctctgccacc cggtctgccg cctttccgaa acgggtgtcc gtcccggcgg
     7861 cgtgttcgga aaagatcggc gtcagctctg ccccggctgc ccggagcttt gccgcctctg
     7921 cgaatgcggc ggaaaaggta cagaacgaac cggtcaccac aaacccgatc cgctcacctg
     7981 tcaagtcgtt catgggcgag tctcctctct tcgagaatgt tgcaaatggt gtctgcgatg
     8041 atctccccgg cggtcatggg tgcaaccttt cccggagcgg cggtgcggcg ttggcacatt
     8101 ttcagtgcaa aaccgcctcg ccgtggtcgg gatagagccg cacctcccgg agcagcaatg
     8161 ccgccgcctg tctgcgctgt tccagcctgg cagtctccca ccacgccgcc cagctgccgg
     8221 caggtgtttc cgttcgtccg gtcgattccc ggcgcagctg ctgttccagt tcccggcgct
     8281 cctgtgccag gcggttcagc tcttcccgga gaaaatctgc cgccgccgtg ggctgggaca
     8341 attccgccgc caggcagcgc atccgctgtt ccagttcctc cagcgccacc gccgccgggt
     8401 cgagctgggg cgttgtctcc gcatagggca aaagctgttc tgcctgctgt gccagaatgg
     8461 gcgaaacggc gtcctccacc cactgggtgc gcagaccggg gatgccggga cagatgccct
     8521 ggcgcttgcc ccggcagacg aaataggtat acgctgcgcc cttgccgttg tttcgggcgt
     8581 agcaatgtgc gccgcagaga ccgcaaaccg ccagcccctg gagccaggag cgatgacctg
     8641 tgccacggtt ggtacagccg ccacgagccg ccagccgttc ctggacgctc agccacaggt
     8701 cgctgtccac gatgccgggg tgaagtcctg ccgccatatg ttctccctgc agccgggtca
     8761 gctttgcgcc gtggcgtgcg gctggactgc cgtagaccag gtagccgttg ccctggcagt
     8821 actgttccag cggatgggtc agttccacgc cctgttcctg caaaaagaca gcgctccgga
     8881 ggtcgccccg gacatacacc gggctgcgca ggatacgcaa aacggcgctg ttcgaccagt
     8941 tcgccccgtt tcgggtgcgg ctgccggaac ggtttgccgc ctgcaccagc tgttccacgt
     9001 tctgcccggc gatgccgtag cccgtataaa acgcctgcac ctgcgccgcc tcgtcgggct
     9061 gaacttccag aatagtggcg gtgtggttcg agatgcggac gtttttcccg gtgtatccat
     9121 acggcggcgt tccgcccagt gccagctgct gggcggctct ggcgtagtaa ttgtcccgga
     9181 ctctaccggc gatggttttc tgctccagct ccgcaaacat catcagcaga ttcagcagca
     9241 ttccaccgat ctccgtccgt gtgtcgaaac tctcccggac ggagtggagc gtcacattcc
     9301 ggcggcggaa cgtctgcatc attgcggcaa agtcgcagag ggaacgggaa atccgatcca
     9361 gcttatacac atagacgctt tccacttcgc cctgttccac cgcctccagc atttcccgga
     9421 acgccgggcg gtgggtgttg gttccggtaa agccccggtc agtaaacaca cgcacctccg
     9481 cctcggcagg acagatgcgg cggcagtgtt ccacctgcat ggcaatagaa atggaatcgg
     9541 ggcgctccac cgactgacgg gcataaattg cgatcatgat gctatcacct cgctatactc
     9601 tatgcgccgg aaaaaacaaa gaggacacct cccgaaggag atgccctctt ttcacgcata
     9661 aaatgcgtta agattcagtt gcgctatttg ctgctatcgc agcgaaagct tactttctct
     9721 tcttagctac cagtgctacg ccaccgatta cagctacagc tacagctacg ccagctaccg
     9781 gcagagcgtc gccagtcttc ggagaagaag tggacttagc agcagcagcc ttcttagtgg
     9841 ttgtagtagc agcagttgtg ccagttgcta ccggagcagt tgtcggagct tcagtcggag
     9901 catcggttgc atctgcaacg atagtgatgg tgcctgcgcc agaaccagcc tcaccagttt
     9961 caccggttac accgttcttg tattccataa cagtaccgtc agaatcctta ccagcaatgg
    10021 tcaggttgaa tacatcgcca gccttagctg cgtcagttac cttaacggtg atctgagcga
    10081 actgacccgg cttcagggtc ttaagcggaa cgcttgcata gctgaacggc atgaatacga
    10141 agccgttgct ctctactgcc aggccagatg cgcccttagc agttgtttcc ataccatctt
    10201 caacatagga caggccttca cccagggtaa cgcctacgcc tacgctgttg aattcagcgg
    10261 tagaaccgat cggcagagtt accttgtaat cagcagcctt cagttcagcc agagtcatag
    10321 tagcactacc gatagtcagg gacatacctg cagcacttgt cggaatagcc ataacagcag
    10381 cggatgcagc tacaacagca gcagcagaaa gagctgccat tgtctttttc aatgtagtct
    10441 tcattgtttc tatttccttt caagaataaa attatatgtg at
//
LOCUS       DAWENO010000068        10350 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2719_length_10350_cov_11.949004, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000068 DAWENO010000000
VERSION     DAWENO010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2719_length_10350_cov_11.949004"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2763 a         2506 c         2886 g         2195 t
ORIGIN      
        1 gggagaaggc aacggaattt ttgaatattg tgcttccaaa aatagcaacg gtggagaaac
       61 ctacgttatt tcaagtgatg taataagtaa aattagtatg ataaaaaatg caaagattaa
      121 atttgagaat tggggaagta atgctaaagt agaggctatc aagtttgtat atgcagataa
      181 ttctgaatta gccaaagctg ttcggtatat gtcgagaaaa gaaaatattg ctgcaatttt
      241 agattcagaa ctttctcatc atgcagttac aacttctgct agagaaacta gctttaccac
      301 cacccctcaa accaccacaa caacaaccac taccaccacc gccaagccca tcgccgccag
      361 cgaaaacggc aaaaacgttg gcctggacga aaccagtgcg gctggctgtg acatcacatt
      421 cagcgaatct ctgttcaacg aaagcggcgt gtgctacttt accatcacgc ccaaggcagg
      481 ctacaccctg tccgacggct ataccgtaaa ggtcggcaca gaggtgcttt ccccgaagaa
      541 caactacaac cccaagaccg ggcagtggtt cttctggagc aacaagagca tgacggacta
      601 taccatccac gtggagggta tttctccggc ggcataacga aaaaaggcgc attcccggat
      661 acgttccgga aatgcgcttt tcgtttattg atacactctg attaccgcag accgcacagc
      721 tgcaagtaca ggtttgcaat gggttcgccc accaccagcg ccagcgccat gccggcagac
      781 agatacggcc caaaggcgaa tctggagtcc ccggtctttg ccttgtggat cagcccgaag
      841 atacagccca ggatcagtcc caggaacagc ccgactacaa cgccctttac tcccaggaac
      901 agtccgccca ccagcatcag ttccgcatcg ccacgcccca tggcacgctc gccggacagg
      961 atcaccagca ggaacagggg gacggacgct gctgctgcgc caatgaagtg ggagagcagg
     1021 gtgctgtttc cggtgcagac atcatagacc gtggcagcaa tgcccagcag cccaatgaat
     1081 cccagcacat acagattgat gagctgggtg tccagatcca tgaaaaagac gaccaccagc
     1141 gccgaacaga acaggcagac cagcccggca tacagcggac ggtcaaagat gccgaagtat
     1201 gcgtagatca gcacgtatag cacggcgttc agactctcca ccagcgtata gcggaacggg
     1261 atctttgcgc cgcagctgtg gcattttccc ctgagccggc accagctgaa aatcgggatc
     1321 agatcccgtt tccggatggg cgtgccgcag gtcatgcagt gggagttgtt ccgccacagg
     1381 gattcctcct tgggcagccg gtagatacag acgttcagaa agctgccgat ggctgcacca
     1441 aaggcaaaga tgatgcagta cagcagcgca tagcataggt aatacataaa gcagattcct
     1501 ctctttttta cttgctttat tgtatcacac tttgaaaaat gtgtcaagaa atatggaaaa
     1561 ttcgtgtaca gcagaaaaag tgaaacactg tgcggataca gcggaaaaaa cacggcaagc
     1621 cccttgacga aaccggcaaa aagtagtaca atattgtaag aagccggtgc attgccggtt
     1681 agcatttatg ggaggtatac accatgtctg aaaagtttat tgttgaaaca tctgcaagac
     1741 acgtacatct gactgccgag gcatttgctg tgctgttcgg tgagggaaag gaactgacag
     1801 ttaagaagat gctgtctcag ccgggacagt tcgcaagcga agagcgtgta actatcgttg
     1861 gtccgaagaa ggaactgccg aacgtttcga tcctcggccc gttccgcagc gcaaaccagg
     1921 tagagctgtc tgcaacagat gcacgttcta tcggtattgc agcaccgatc cgggaatccg
     1981 gtgacgtagc aggctccggt gcatgtacga tcgttggtcc ggctggtcag ctggagatct
     2041 ctgagggcgt gatcgtggca aagcgtcaca tccacttcac cccggaggat gcagcgaagg
     2101 ctggcgtaaa ggacaaggat atcgtttggg tacgtgttga gaccgacggc agaaaggcaa
     2161 ttctgggcga cgtggtttgc cgtgtttccc cgaactatgc aactgcaatg cacatcgata
     2221 cagacgaatc caacgccatc ggcgcaggcc gcaatcagat gggcgaaatc gtcaaagtgg
     2281 actaatcata ttttttgtaa gagccggaag gttcgaaaga aaaacggcgg atgctgaatc
     2341 cttttggcat ccgttgtttt tttagaaagg aacaacatta tgaaatcatg gaaaaaactc
     2401 acggcactgc tgctcgccgg cgttctggca ggctcggcgt ttaccggatg cggcaagcag
     2461 agcaatgaca gcagcaagcc ggaatcttct gccgcagagg aatcctccgt caaggagacc
     2521 gaagccgcag cggaaaccac cgaagcgact accactaccg tacacgtaag ccctacagag
     2581 acggtttcgt ctacgctggg tacggaactg acggatatca acgccatgct ggacaagggc
     2641 agcgacaccg ccaacaacta taaggcaaag ctggacaact ttatccagga aggcgacgtg
     2701 gtacagtcct ttacctttat cgtctattct gccgacggct cgtccagtct gggcgacttc
     2761 aaaggcggct acggcgtctc cgtcaaggac ggctgtgcca gcgcaaccga tgccggatgg
     2821 tatcaggcgg cagactttga acagtcccag aacagcgcct atgcggaatt cacctggaat
     2881 gttcccacag agatccagac cgagatcgac cccagcggcg aagtcctgtt tggtcactgg
     2941 tggagcaatg tacagcaggt aaagctgtcc agcattgtct gcacctatac ccgcaccaag
     3001 gaagttccgg tagacggcac caattctgtc agcccggcat ccaccctcag ctttaacgat
     3061 gaggcagcca aaacggcgaa gatcgcactg tctgatctga tcggtgcaga cgacaccctc
     3121 cagaccgtga cgttcgatat cagcagcagc ggcgcactgg gcaagttcac cggcgcattc
     3181 ggtgtgtctg tggatgagaa tgcagactgc gcaaccgata agggctggta tcagaccaag
     3241 aatgtctgcg tgtttaccga ttcttccagc ctctcgctga cctggatcgt gccggatgag
     3301 gtcaaggaga gcatcgacaa cggcggcgag gtgatgctgg gatactggtg gagtgaccag
     3361 cccagcatta cgctgaacaa tgtgagtgtc cgctacagca acagcaccgg aacgaccacc
     3421 aagacggaga ccaagtcgga ggaatcctcc aaaggcggcg aagtgacaga agtgaccggc
     3481 gatattccca ccaaggatca ggtcaatgcc atgagcagca agcagatcgt ggaaaacatc
     3541 aaggtgggct ggaacctggg caacagcctg gacagccacg acaccaattc cagcgataca
     3601 gaaaccggct ggggcaaccc caagaccaca cagcagatga tcgataccgt aaaggctgcc
     3661 ggctttaatg cagttcgtat tccggtcacc tggggcgagc atatggcagc agacggcact
     3721 attgatgcgg catggatgag ccgtgtcaag gaagtcgtag actatgccta taacgacggg
     3781 ctgtatgtca ttctgaacgt acaccacgac gacgaactct ggctcgtccc caccaaggat
     3841 aagctggaaa gcgacaaggc tacgctgacc accatctgga aacagatctg tgccaccttc
     3901 caggactatg accaccgtct gatcttcgag ggcatgaacg aaccccgtgt catcggctct
     3961 gccgaggagt ggaccggcgg cacccaggaa tcctatgatg tcatcaacca gctgttccag
     4021 gcgtttgtgg acaccgtccg tgcatccggc ggctctaaca cagaccgtac cgtgatcgtc
     4081 accgattacg cacagtctgc ggagaagaac gccatcggcg gtatgatcgt tcccaaggac
     4141 gaccacatta tcgtttccct gcacatctat gcgccgtgga atttctgcgg tccggacgac
     4201 acccgtgccg actggggcag cgacagcgat aagcaggaac tggacaccac attccagtac
     4261 ctggacgata cctttatcag caagggcatt ccggttatca ttgacgagac cggctgcgtc
     4321 aacaagaacg agaacacctc tgccagagtg gcgtggttca gctattatct cagcgctgcc
     4381 aagaaacacg gcatcaagtg ctttatctgg gacaacaacg aaacgaaaaa cggtttcgga
     4441 ctgctgaacc gcaaggactg cacctggtat tatccggaga tcatccaggc gatccaggac
     4501 gctacaaaat aaagctgaat gttggcgaat tgcataaatc attcaccgta cgaatgtata
     4561 aaacgtagaa tttctataaa aactgcaaaa aaattcgtct gaaacgtgca aaaaatcgaa
     4621 aagtatgcta taataagaga aagcactggg aaagggaatc ctgttcctgt ttctcaaact
     4681 agaggaggca ttttactatg agtcatcgaa tcattaccat tgaaagagaa tatgcaagcg
     4741 gcggccgtga gatcggcgag atgatcgcag agcggctggg cattccgtgc tacaatcgtg
     4801 aaattttaca aatggcgtct gaacgctgca acgtctccat ggagtacctg gagactgcgg
     4861 aagaagcagc gccgaagagc tttttataca cgctgatgct gacctccagc cccacacgca
     4921 ccatcgaaga gaacctgccg ctgtcggata aggtctatat catagagacc aacatcatcc
     4981 gggaactggc agaaaaaggc gactgcgtga tcgtaggacg ctgtgcaagc tatatcctcc
     5041 gtgatctgga aaatctgttc aatgtgttta tctatgcgga caccaagtcc cgttcagaac
     5101 gtgccgtgca ggagtatcac gtagacgaac gccgggtaga ggcaatgctg cgcaagatcg
     5161 acaagcgccg tgagaccttc tacagcatca ataccggcgg aaactggtac gacaaggaca
     5221 attatgacct ctgtctgaac agcggcgcac tgggactgga gctgtgtgcg gatattgtgg
     5281 tagagacggc gaaaaagacc atctaccgct gatttttcca aagagaatcg aatttgcaga
     5341 aacaggacgg aggcacacgc tttcgccctg tttttttgcg gcgcagcggc agaaaacagg
     5401 aaaaaacagt gttgacagat tgtaaaaaat ggtgtaaaat aaaagagaaa accagtttcg
     5461 ctttgggtac gggactggta cgcagaaagg acggtgattt ttatgaagaa aggaaaggtc
     5521 gtttccatcg tgacagcagg gatcatggcg ctggctgcac ttccggcact gccggcgggt
     5581 gcggcggaag agatctactg tctgggagat gtggacatgg atcacgtgat taccgggcat
     5641 gacgccgcta tggtgacacg gtatctgaat gtagatccgc atctgctgac agaggaacag
     5701 ctgatgctgg cagatgtaaa cgaggacggc gtggtggatc agagcgatgc cgactggatc
     5761 tacgaaaatc agaggtattc gctgggagac agtttcaaga taggcagaaa agaccggggc
     5821 ttgatgctgg attccacaac actgtatttt gagctgtatt actgtgcgag ggagtatgcc
     5881 ggttatacac atacggtggt caacagcgca tccgcagttg tcacgaccca tgaaaagtgg
     5941 acggaaaacg aaaatgcaaa tatcgtcagc aagctggact ataatctgat ggatgtagac
     6001 gcagacggcg atgtggacat ggtagatatt tttgtgacgg cgcagtcttg tgcgctgaga
     6061 ggtgcaggtt atctgaaaac cggctttttt gcggacggca gatatgacct ggatccggcg
     6121 cttttggatg tgtcaaaact gtgtaccata aacggattag gtcttttcga tcagttttaa
     6181 tggtctcatt tttagggact gcatgagctt catgcagaaa atgagagcat aggcaatatg
     6241 aaggaggttt ttcatatgaa aaaaagaaag gtgttatcat tcctcacagc ggctgttctg
     6301 acagtcggcg cagtgccgtt ctctgtgatc catgcagctg cggcagatga gacttatgtg
     6361 cagggcgatg tagacatgga cggttatatc accggacatg atgcggcaat ggtcagccgg
     6421 tatctgaacg tagacccgga actgctcagc gaccagcaga aggcgttggc ggacatgaac
     6481 ggtgacggcg tggtggatca gagcgacgca gatgctattt ttgcgcagaa ggtctgcggc
     6541 ttaggcgtta cgtttcagaa aggtgaacgt aaaaacccga atgtaacgga tgtatacatg
     6601 gtacgatatt atagttacac gagcaattac agggactata aagtagtagc agatcattct
     6661 ctggaaaaag atgatattga tgatataatc gatcagctgg aaagccagcg ggtaatcagt
     6721 ctgagagatt ttaacctgat ggatgtagat gcagatggtg ttgtgagtga ggatgatgtt
     6781 gaggcattga tttgtgcggt ctcacgcgca ggcgcacaag taaagcccat atatcctgca
     6841 gaagatcgct atgatatgaa tttagtggaa tattttgatg tagaaccggg cgatctggat
     6901 atggatggct atgtgaccgg gcatgatgcg gcaatggcaa ctcgttttct gtatgtagat
     6961 cccacgctgc tgacaaagga ccggcagatt ctgggggaca tgaacggtga cggtgttttc
     7021 gatcaggcag atgccgattt gattcacgag aaacagacct atgccctctg tgatgtacgg
     7081 tacagccgga aatcagaaaa agaaacgatc agctccactg ctgtaatgga tgaactttac
     7141 atggtggctc ttcagggcgt aaaagaaatt ggtcgttccc ttggtattac caacaaaggc
     7201 gataaatttg taaaatcgaa taacgggaag agctttgatg aaagtatggc ggatcgcaat
     7261 attgacaatg agctgtactg gaatctgatg gatgtggatg cagacggtga cgtcgatctg
     7321 gacgatgtct atgcaacaat ggtatcctgt gcattcaccg gcagcggtgc cagcgactct
     7381 tcgttcttct cggagggacg ctatgatctg aactttgatc tgatcgcaga ctatgcgaga
     7441 atccacaacg tccagcttgg aaacgttcag cttggataaa aaacacggta caagtgaatc
     7501 ataaacgagc cgccggcagg gaaaacactg tcggcggctc gcctgttttc cgggcggcgt
     7561 ggaaaggcag atgcaccgcc ttgacatttc acccggaaca cactataata aatctatccg
     7621 gcaattctgg aaattgcccg aaacttttcg aaaggaatct gatttatgaa atcgatctat
     7681 acctgcccgt actgcggcgc aaagtcattc aatccgctga aaaaagcctt taccggcgga
     7741 ctgcacacca aaggcaaagt ttgcatggaa tgcggtcagc actgtgtcaa cggaaaggca
     7801 tccatgattt tcagcgcagt ggtgtacatt gctgccctgg tgctggtgat gctcatttac
     7861 ttcaagggaa attccacctg ggactatgcg tatatggctg gcacgatcgt ggcggcgtat
     7921 gtgttgtgcc ggattttcga tgcctttttt ggtccgctgg aaaagcctat ccggaatgac
     7981 gtgctgagct gatggctgcc ggactttata tccatgtgcc gttttgccgc cggaagtgtc
     8041 cgtactgcaa cttctattcc atcgccgccg aaccggaact gctggaacgc tatacccagg
     8101 cggtctgtcg gaatctgcgg cactatgcct gtgcagaacc tgtggatacc gtttattttg
     8161 gcggcggcac accgtctctg ctgtctccgg cacaaatcgc acagatcctg gaaacggcgg
     8221 cggattgttt caccctgtcc ccggatacag aggtgacaat ggaggcaaac cctgccgctg
     8281 ccgcaccgga acagctgcgc cggttccggg aaagcggcgt aaaccggctg tctttcgggg
     8341 tgcagtccct ttcggacagc cagctgaaac ggctgggtcg gctgcacacg gcgcaggagg
     8401 cggtcgagac cgtctgcgct gccgcagcgg caggatttga caatctgtcc tgtgatctga
     8461 tgctggcgct gccgggacaa acgccggagg aactggaaca gaccatccgg gaaatgaccc
     8521 ggctgcccat tgtgcatatc tccgcctatc tgctgaaaat cgaaccggga acgcctttcg
     8581 ccagacagca gattgcggcg cagtgtcctg atgaggatgc ggcggcagat ctctacttac
     8641 aggcggtgga acagctgaat gctgccggat tcgcccagta tgagatctcc aactttgcca
     8701 agcccggctt tgagagccgt cacaactgca agtactggca ctgcgagccg tatttgggca
     8761 ttggtccgtc ggcgcactcc tgctggaacg ggaaacgatt tttcgtgccg tcctcggcgg
     8821 catcctttct ggaacagccg gtgcagacgg tggaaactga ggacggtgcg ccctgcaccc
     8881 tggaagagcg cctgatgctg ggactgcggc tgacagaggg cgttccggcg gcgtggctgg
     8941 cagaaaaaaa agcggcggtg gagcgctact gcaagctggg attcctgcgg cgcaccggag
     9001 accgggtggc atttacgccg aaggggtttc tggtgtcgaa tacgatattg gcggagctgg
     9061 tgttataaaa aatccctccg caagtataac cttccccatt ccggtgcata ctctcatcga
     9121 ggtgagcaat atgaaaaaac tggaatgggc attgctgctg ggtatggtcg gtgctgtctg
     9181 ctgttcggcg ctgacgggaa cggcgcagag ttaccggtct ctggaacaga acgtgctgcg
     9241 gctgcatatc ctggcgaatt cggacagcat cgacgaccag gcgctgaaac tgaaagtccg
     9301 ggacaaggtg ctggaacagg cggacacact ctttgccggc gatgcggaat ccgtggagga
     9361 tgcggagacc attgccgcag aaaatctgga aaccctggaa cagacggcgc agcaactggt
     9421 ctgggatgag ggctattcct atccggtaaa ggctcagctg gtggaaatgc ccttcgatga
     9481 ccgtacttac ggggactggg tcatgccggc aggggaatac accgccctgc gcatcaccat
     9541 cggtgcggca cagggtcaga actggtggtg cgtgatgtac ccgtctctgt gtgtccccaa
     9601 tgcctgcgat gtcaccgcag acgaagagac cgccgaggac gcttttggaa aaccggagca
     9661 ggatctgctg cggcatcatg aacgctatgc gataaagctg aaatgcgtgg aatggctgga
     9721 aaaatggttc tgaacgcctg ctgcgtgctt ttgcgcacag ataaatgtgt aaataaattg
     9781 taaaatatcg aacggatgct tgacaggcga atgataatat gataaaatag caaaagtaaa
     9841 caaagaagag cattcccgtt ctcaccgttt ggcgtttagt cgaaacggtc aatactcgtg
     9901 tacaacggct tttctctccg gagagaggac ggttttgacg aatggagaag gagtgttcac
     9961 ctggaaaagt ggacgctttt ttttgtttga aaataatatg tttggaggtg ctggcttatc
    10021 agcaaacaag aactgcagat caatgaggaa attcgggaca gagagattct ggtcatcgac
    10081 cagaacggcg aaaaactcgg aacaatgtct gcaagagatg cgcagaagat cgcctatgaa
    10141 aaagacctgg atctggtaaa gatcgcaccg ggtgcaaagc cgccggtgtg ccggatcatg
    10201 gactacggca agtactgctt tgaacagcag aaacgggaaa aagaggcacg gaaaaatcag
    10261 aaggttgtct ccatcaagga gatccgtatg ttttctgcca tcgacaccca tgactttgag
    10321 accaaggtca accaggcaaa gaagtttttg
//
LOCUS       DAWENO010000069        10243 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2756_length_10243_cov_8.689537, whole genome shotgun sequence.
ACCESSION   DAWENO010000069 DAWENO010000000
VERSION     DAWENO010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_10243_cov_8.689537"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2356..2365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8019..8028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2401 a         2634 c         2801 g         2387 t
ORIGIN      
        1 accggtgcag agtatcgctc taccgaccac tacggttacg gctactacga aacgtctatg
       61 aaggcaatca aaaacgacgg cgtggtttcc tcgttcttca cctataccgg accatcggag
      121 agcaatccgt gggacgagat cgacgtggag gtcctgggca aggataccac aaaggtgcag
      181 ttcaactact acaccaacgg taccggaaac cacgagtatc tgtacgacct gggctttgat
      241 gcttctgagg ggtatcacac ctatggattc gactggcaga aggattccat cacctggtat
      301 gtggacggca aggcggttta cactgccacc agcaacattc cggtgacacc cggcaagatc
      361 atgatgaatg tatggccggg catcggcgtc aacgactggc tgaaaccgtt tgatggcaca
      421 acgccgctga ctgccagcta cgaatgggtg acttatcaga aggcatccaa ggcaaactaa
      481 gccaaacttc tattcttcat attcattccc attcttcggg tgccggcacc tgttccacgc
      541 tggtatccat ccgcagtttc ggcttctctg aactgcgatt ccaatgaaac agcgccgcat
      601 ttccgaaaaa gggagatgcg gcgctgtttt gtgttgttga atcaaattac gaaaaccgtg
      661 tgctgttttg aaactgccgg cggtattcct tcggcgtcat gcctacgtat ttctgaaaca
      721 ccttgatgaa atagctctgg gagctgaaac tgagcagact gctgatcgcc gatgccggct
      781 gctgggtgta ttggagcaga ttctttgctg cctcgatctt tttgatggcg atgtattcgc
      841 tgatactgac gtgcatttct ttctggaact gtcgggagag atggggcgca ctgactgccg
      901 ttgcatctgc cacatcctgt aagaaaatgc ggctgtgcag gtgaatgctg atgtaatcca
      961 tagctttcag caccagcggc gaatgctgtg cggtgtcctg caggcggcgc atctttcctg
     1021 taaagtcctg tatcacctga taatgcagca cgcgtatctg cggttcactt tttgctgcat
     1081 cggtgcgctg aatatacagg tcgctgagac tgaacgcctc ttcctgcggc ataccgccct
     1141 ggatgcagta gcgtgtcagc atggaaatgg tgatgatcag gtgatatttc agattgcgca
     1201 gcggatcttc actcagcttg ccgtaaccct ttccacccag cggcgagtac agcagatgca
     1261 ccgtgtcgct gttcccggaa acgacacttt caaaaaaggc aagttccgtt cccagcatag
     1321 cgtgtgggat ctcctgttcc cgctgtgccg tcagaaaatg ggtgaaatcc tgttcagcgg
     1381 tctggttctg taccgctgtg taaagctgta aaaactgccg tagctgttcc aggcgttcct
     1441 ggttgagtcg gaattgcttt ccgtcccggc acaggaatcc gaaatcggtc atggcatcca
     1501 gcagtgcatc tgtggggaca tcttccggca gtgccttttt cagtgccgtg cgtgtctgct
     1561 ttccgtgctg aatgctgtac agcaagaact gcaccgcctc agcgtgggac aatatttctt
     1621 tcagttccat ggcttatgct cccacgatca tttgccgcag ccgtgccaga tcgagtccgc
     1681 tgacactgcc gtccgggaaa atatctgccg cctttgcctg gtcggcttgc agtacgccgc
     1741 tgcccagcag gtaacgctgt aagagcagta aatcggcaag atcggttttg ccgtctccgt
     1801 tgatgtctcc ggcaacaggc tgttctgctg tggaaagcgt cagctttttg aatacggttt
     1861 tccagtcgga tgtggtgtag agacagtaga tggtatggtc tgccgccgcc agaaccggct
     1921 ggttcaccgt ctcctgtgcg atgggatttc ctgcctgtgt ccaggtctgt gccgtcttgt
     1981 ccagctcaca gaggagaaac tcgcccggcg actgggcatt gactgccagg taaaatccgc
     2041 cgtccgctgc tgccggtaca agctggaacg ctctgtcttt ggcggtatcc ggcagggggt
     2101 acgaaatgac agcgccgtct gtccagcggt acaggtcggt ctttccgccg gagggtgcga
     2161 tccagatatc ttcgccgtct gccagaacgc tgaccatgcc gcagtcctgg tcggacaggg
     2221 ctgtgctgtt ccagctgctg ccgttccaca cagccgcttt ggggacgccg ttgtcatcta
     2281 agtcccggta gccgatgagg atcgtttctc cggagacagc gatctccatg ttgcaggcat
     2341 ttgccgcaat ggtgcnnnnn nnnnncctgt aaagcgtagg ggaatgtgcc ggtggtatat
     2401 gtgagataca gcttgccgcc ggatgcagtc atgtctgcat actgtgccag ttctgtcccg
     2461 gtatagcagg tttcccagac agtgccgttc catcggcaca ggcgcaggag atactggtca
     2521 tcctggtaga gcaggtatgg ggtgttgccg gcgaaagcaa gtttggggtt gtagacactg
     2581 ccggaaatgg cagtgctgac aggtgtgagc gtgtccccgt ccagacggta cagcacggtg
     2641 ccgctgtccg aggcggaagc cagataaacg ctgccgtcac tgccggtaca catatcatag
     2701 ctctggagtt cctgagaggt gggaacatcg cagacggact gccaaagttg acggtcggag
     2761 acatccgcaa aggatgcgga aaaggtcagc gtgccgtccg gctgtatctg gatatcgtcc
     2821 agggcaatgc cgctgtttgt gccgtcgctg tagaccagcg caccgtcctg gaacgttttg
     2881 gtcaggtcgg ttgtgccgat ggaatccggt gcatttgtgc cgccgtagca ggaacgggtg
     2941 aggtcacctt gtccggcatt cagtgccgtt tcgccgggac ggaatacgta aatgccgtcg
     3001 gtgtcgccac ggtagttccc ctggacagcc gtgttgatgc ggtagacgac catgccctcg
     3061 ccgtagattt tggtgtccag ctcatcggaa tactgcttgc cgggcttgcg gtattccaca
     3121 gcgaaaaatt ccgtgtcgga gaacggcgtt ttcagcaggt acagccggtt tccgctgtct
     3181 gaggatgccg gctgtaaggt gtacgtgccg tccttggtga tggtttcagc atccagccag
     3241 ccggaaaccg catagcgctg gtaggcaagg ggatattgca ggaaaatgga gtttgtcgcc
     3301 ataatgtccc actgtcccac gggatttccg gtagaagtgt ccttgcggta taagtcgggg
     3361 tagcccatgc tgtgcatgaa ctcgtggcag agcgttccca cgccgctgaa tatggtggaa
     3421 ctgagcaggg tatagctgct gagcaggttg atactgctca ccggcgtacc gttcagttcc
     3481 agaccgttga catgaaacgc ctttgcccag agcggatcgc tcatatcctc tgcctgacca
     3541 tccaccacta aagtcacgtt gtcgatcaca ccgtcttggt ttccgtccag gggcgtgtcc
     3601 gcagaaacgg tgatattctg taccatttcc agggcatact ggttgtagtc ggtatagccg
     3661 aacttttcct gtttcagcac atagggctgg atcacgccgt cctgcatctg gggaaaatag
     3721 gaggtgacgt gaaccttgcc gtaggaaatg gtgtccatgt attttgccag ggaacgatag
     3781 gtgtcggtgc tgttgcacat ggcgaccatg tcctcggtct tgtctgccat aaagttgtcc
     3841 gtatcagcgg aggcgaactg tgcaaacaaa acgatattgt tcacctgctg cacactgtcg
     3901 gcagcctgtg cggaaatgcc gccgaacggg actccggcaa gacacagtac tgcactcagc
     3961 atcactggaa atggtttcgg gaatctatgt tttttcatct gttttctcct gtccggacag
     4021 gtttcgggct gtcctggtat aatacttcta ttataccata cggcggtgca tttccgcaat
     4081 ggaaacactg cacaaaaccg agggcgattc tttggcagaa aagctttttt tcggaaattt
     4141 gcaaaaaaca cttgacaagc ttttcttcgt cgtgtataat agcatatgta acctatcaaa
     4201 aacataggat ttagaaaacg agaggagcgg acgaaatgga aaaacacacg ttcggaaagg
     4261 aatacggctt tcagaccaga gccgtccaca caggcaatga cgttgacgca gagacgggtg
     4321 cgatcaagcg cccaatcacc atggcgaaca gctatgctct gccctatgac cccagcgatt
     4381 tgaactggag cagtgccgga aagaatctgt atacccggaa cggcggctcg aaccagcagt
     4441 atttgcagga aaagctggca tctctggagg gcggagagga ctgcatcgtg ctggcgagcg
     4501 gcgttgcggc actgtcgggc ttgttctttg cactgctgga aagcggcgat cacgtggtat
     4561 tttccagtgt gacctatatt gcggtgtacc gtctgctcca tgagcttttg aagaacaagt
     4621 tccacgtgga caccaccatt gtggacacct ccgatctgga ggcagtgaaa aaggcgattc
     4681 gcccgaacac caagctggtg catatcgaaa cgccgggaaa tcccacgctg actatctcgg
     4741 acatccgtgc cattgcggac attgcccatg aaaacggcgc attgctgtcg gtggacaaca
     4801 ccttcgcatc gccctataac cagcgtccca ttgaactggg cgcagatttc accattgaga
     4861 gtctgaccaa gtatatcaac ggacacggcg atgctatggg cggtgcgatc atcggcaaga
     4921 aggagtatct ggatatcatc cgggcgcagt cgcaggtcaa tctaggtgcg accatcagcc
     4981 cgtttaacgc atggctcatc atgcgtggtt ctgtaactct gccgctccgg atgcgtcagc
     5041 acaacgaaaa tgcactccgt gtggcgaagt atctccagac tgtaaagggt gtcagctttg
     5101 tggcatatcc cggactggaa agccacaagg gacatgacat cgccgtaaag cagatgcagg
     5161 gcggattcgg cggcgtgctg tcgttcggac tgcacgcaga ccatgatact tacaatcgct
     5221 ttgtcagcca tctgaaagtt atcacctcgg cagtgtctct gggacatgac gagagcctga
     5281 tcgtgttcct gggcgaaaat gatgagcggc agtatttgta tccggaggag ttccacggcg
     5341 gatttttccg gttcagcgtg ggcatcgagg atgcagagga cattatcgcc gacctggaac
     5401 aggcgtttgc aaaagagggg ctgctgtaaa tggcattgaa cgcattttca agaacagaac
     5461 tgctggtggg aaagccggca atggaactgc tggcgcaggc gagagtggcg gtattcggca
     5521 ttggcggtgt cggcggctat acggtagagg cgctggcacg gagcggcgtg ggtgcgctgg
     5581 atctcatcga cgatgaccgg gtatgcctta ccaatatcaa ccgccagatc tacgccaccc
     5641 ggaagaccgt agggaaatat aaagtggaag tagcaaaaga acgcatcctc cagatcaatc
     5701 cgaactgcca ggtcgagaca cactgtgcct tttatatgcc ggacacagcg gcgcagtttg
     5761 attttacaca gtatgactat gtggtagacg ccatcgacac tgtgactggt aagctggaga
     5821 tggtagagca ggcataccgt gccggcacgc cgcttatcag ctccatgggc gccggaaaca
     5881 agatggatcc cactgcattt gaggtggcag atatctatga aacatcggtc tgcccgctgg
     5941 caaaggtgat gcgccatgaa ctgcgaaagc gtggaatccc cgggctgaaa gtggtctact
     6001 ccaaagaaaa gcccattgcg ccaaaagagg acatggcaaa cagctgcaag acccactgca
     6061 tctgtccgcc gggaaccgcc cggaagtgta cccagcggcg gcaggtaccc ggcagtaacg
     6121 cctttgtgcc gtcagtggtg ggacttatca tcgccggaga ggtgataaag gatctggtcg
     6181 gctggaacag ctgacagcct gcaaaataaa gcatatagaa aagccgtatc acacaccgga
     6241 ttttccggca ggtggtacgg cttttgtgtt atcgtaattt tagaacgaaa cgaccagctg
     6301 cattttgaac tgttcttctc cgtagacagc atggggaatg tccttgggca taatcagtgt
     6361 ttcaccggcg tggagcagga atacttctcc gccgacggta aactttccgg taccctccag
     6421 cacagtcacc attgcgtcac cgccggcagc gtgcgtggag atttcttcgc ccttgtcaaa
     6481 ggaaaaaatg gtcatgctga ccttgtcatt ctgtaccagc gttttgctga caacctgacc
     6541 ggtctgatat tccaccagat ctttcagctg caaaacagcc tgcttttcaa tgttcttgta
     6601 catggccttt accctctttc atgcttttcc agccagtgtg ccgcatagga acacgtcgct
     6661 gtcacctgtt ttccgtctgc ccggatctgc gccacggcac gggagaccag ttctccggca
     6721 atgcccatac ctcggagaga atcgtctacg aatgtatggt caatgcagca tacgccgggc
     6781 gttgtttccg gaaaggtgat ctcagccaca cattttccgg cagcatctgt gcaaaagatg
     6841 cggttcggtt cgctttgata ttccatagaa tgcctttctc agagtgcctt tttgatggca
     6901 gccagcagat cgtcatagga ttcgaacgcc ggcagttccg gatagtcttt cttgatctgt
     6961 tcggtgctgc ggaacaggaa tcctgccttg cttgcctgga tcattgccag atcgttgtgg
     7021 ctgtcgccgc tggcaatcgt ttcatagcca atggactgga gtgccttaac ggtggtcagc
     7081 tttgaatttt cacagcgcat cttgaaaccg gtgatctcgc cgtccggcgc aacttccagg
     7141 gagttgcaga aaatggtggg atagcccaac tttttcatga gcggacctgc aaactgggtg
     7201 aatgtgtcgc tgatgatgat gacctgtgtt gtggcacgca gttcgtccag gaactccttt
     7261 gcgcccggca gaggatcgat ctttgcaatg gtttcctgga tctctttcag gcccagaccg
     7321 tgttccttca gcacccccag acgccagtgc atcagcttgt cataatccgg ctcgtctctc
     7381 gtggtacgtt tcagttccgg gatgccgctc tcttctgcaa aagcgatcca gatctccggt
     7441 accagtacgc cttccaaatc caaacaaaca atattcatag tatgtgattc ctttcctttg
     7501 cccgatacaa ccatattttg tgcattcgga cgttgtttac tcttgctatt ataacacact
     7561 cagccggaaa atgcaaccgc ttttttccgc cactttcccg tccggtactc caaaaacgag
     7621 ataagccagt tggaaaacca gccgatgggt acggcgcacc acaccatctg aatgccgaac
     7681 cggggcgcca tggtaactgc gaatattacc cggatgctca gattcaccag attggcgatg
     7741 gtgaacattt tcatgtcgcc tgcaccacgc agcagcccgt ccaccgccat tttgaacccg
     7801 atcagacaga agaaccagcc catgaatgtg agatagccct cgccggtggc gtatgccgca
     7861 gccgtgccgt ctgtccccag gaacaatgtg accagcggct ttgccagcag ttctacgcaa
     7921 atgcagatca aaacggcact cgccagcacc atcttgttgg ctgcgtggta gccctttacc
     7981 acacgctcct gttgttttgc accaagattc tgtgccgtnn nnnnnnnnag ggcattgccg
     8041 aacgctgtca tcggcacaat gcaaatggac tctacacgca tcgccgccga gaatccggca
     8101 agtgcctctg aaccaaagct gtttaccaca gactgcacca gcatcattcc gatggaaacc
     8161 gttgactgct ggagaataga gggcagggcg attcgtgtca ttcccatcag ttctgtccgg
     8221 ctgaatacac cgggtttctc acagggaaaa cgccgcagtt cccacagcag cacgccgaaa
     8281 gaaagtactg ccgaaattcc ctgtgcaatc agcgttgccc acgccgcacc agctacgccc
     8341 atatcaaagc gagtgacaaa gaccatatcc agcgccacgt taaatacaga ggaaaaaatc
     8401 agaaaataca aaggaatcct ggattttccc agagcgttga acatggatga gaatacgttg
     8461 tacagaaaca aaaaaggcag ccccaggaag tagatgttca gatattccac agacattccc
     8521 agaacatctt ccggtgtttt caagacctcc atgatccagc ggctcagtcc aagccccagt
     8581 ccgcccagca ggatgctgac acccagaaac gccagacagg cagtataaag cgccagtttc
     8641 atccgcctgt attcctttgc gccgaaatag cggctcactg ccaccgatgc gccgatgccg
     8701 ccgccaatgg caacgcaaat gaaaatgtta gtcagcgcat aggaggcgcc aactgccgcc
     8761 agtgcctgtt cgcctacata gcgtcccaca atggcagagt ccgccagcgt gtacagctgt
     8821 tgaaacagat ttcccagaac catgggcagg gagaaaagaa gcagcgccgg caggggcttt
     8881 tttcgtatca aatagtcgtt ttccatgatg tcttacatac tccttgtcaa aattactgta
     8941 atataatcag tataggacga gcatcgtgga aaagtcaacc gtttcttatg aacaaatcaa
     9001 gttgcggcac agagggagca atcacactgt agttagccta gactaaccaa gattaggatg
     9061 attggtaaga aatttacaga aaatgcttga ttcctgccgg aagatggtgt ataatagagg
     9121 acgtatgcag atcgtataca atgccatatc atgaaaggag tttgaccatg atttattcaa
     9181 acgaagtaaa cgaaatgtgc tgcgtaaaac agggcgttca gcacggtgct gcaccgattc
     9241 ctgaggaagc aaagtgggta aaggcaaagg aaatctctga catttccggt ctgacacacg
     9301 gtgtgggctg gtgcgcaccg cagcagggtg cctgtaagct gaccctgaac gtaaaggaag
     9361 gcgtaatcca ggaagctctg gtggagacca tcggatgttc cggtatgacc cattccgctg
     9421 ctatggcagc agaagttctg ccgggcagaa ccattctgga ggcactgaat acagacctcg
     9481 tttgcgatgc gatcaatact gcaatgcgtg aactgttcct gcagatcgta tacggccgta
     9541 cacagagtgc attctctgaa gaaggtctgc cgatcggcgc tggtctggaa gatctgggta
     9601 agggtctgcg ttctcaggtt ggtaccatgt acagcactct gaagaagggt ccccgctatc
     9661 tggaaatgac cgatggctat gttaccaaga ttgctctgga cgaagatgat gagattatcg
     9721 gctacaagtt tgtaaacttc ggcaagatga tggacttcat caaggctggt gacgatgcaa
     9781 acactgcttt cgaaaaggca cagggtcagt acggccgtgt tgctgatgca gtgaagtttg
     9841 ttgacccgag aaaagaataa ggaacaggag gaaacacccc atggcattat ttgaatcata
     9901 tgagagaaga gaaaagcaga ttctggctgt tctgaaagaa tatggcatcg actctattga
     9961 gggcgctgct gaagtaacca aggcagctgg cctggatgta tacaagatgg tagagggcat
    10021 tcagccgatc tgtttcgaaa acgcaaagtg ggcttatacc gttggcgcag ctatcgcaat
    10081 caagaagggc tgccgcagag ctgctgacgc tgctgctgca atcggtgaag gtctgcaggc
    10141 attctgcatc cctggctctg ttgctgacca gagaaaagtt ggtctgggtc acggtaacct
    10201 gggcaagatg ctgctggaag aagacaccaa gtgtttcgca ttc
//
LOCUS       DAWENO010000070        10195 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2774_length_10195_cov_10.682347, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000070 DAWENO010000000
VERSION     DAWENO010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2774_length_10195_cov_10.682347"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1346..1355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3268..3277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4312..4321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2404 a         2909 c         2614 g         2238 t
ORIGIN      
        1 tggcgaagaa cttcttcgcc ggtttccgga tcagtaccct tgatagacgg tacatccagc
       61 ggagacggca tataatctac gatagcgtcc agcagtttct gtacgccctt gttacgatag
      121 gaagtaccgc aggtaaccgg aaccatcttg tttgcgatgg tggacttacg gatgcaagcc
      181 ttgatctcgt cgatggtgat ttcttcatcg ttaaagaact tctccatcag agcgtcgtcc
      241 tgctcagcaa cgtgttccat cagttcctgg tgatattcct cagccttttc cttcaggtca
      301 tccggaattt cttccacacg gatgtccttg cccaggtcat catagtaaac ataagccttc
      361 atctcaacca ggtcgatgat gcccttgaat tcgtcctcag agccgatcgg cagctgaatc
      421 ggaacagcgt tacacttcag acgctcgtgc atcatgttga ctacgttgta gaagtcagcg
      481 cccatgatgt ccattttatt gacataaacc atacgaggaa cctgatactt gtcagcctgt
      541 ctccatacag tttctgtctg cggttctaca ccgccctttg cgcacagtac agtaacagaa
      601 ccgtccagta cacgcagaga acgctcaact tctactgtga agtcaacgtg tcccggggtg
      661 tcgatgatgt tgattctgtg acctgcccag tgtgcagtag ttgcagcaga tgtgattgtg
      721 ataccacgtt cctgctcctg ctccatccag tccatagtag cggcaccctc gtgggtctcg
      781 ccgatcttgt ggttgatacc ggtgtagaac agaatacgct cggtagtggt tgtcttaccg
      841 gcatcgatgt gcgccatgat accgatattt ctggtttttt ccagggaaca atctctagcc
      901 atagatgatc ctccttatac agcagcgcag tgcgcttacc agcggtagtg tgcaaacgcc
      961 ttgtttgcct ctgccatctt gtgtgtatcg tcacgcttct tgcaggcacc gccggtgttg
     1021 ttcagtgcat cgagaatctc tcctgccaga cgctccttca ttgtcttttc gttccgcagt
     1081 ctggaatact tggtgatcca gcgcagaccc agagtctgac ggcgttccgg acgaacttcc
     1141 atcggaacct ggtatgtggc accgcccata cggcgagcct ttacttccag aacaggcatt
     1201 acattgtcca tagcctcctg gaacatttcc agtgcatcct tgccggtctt ttcagcaacg
     1261 atgtcgaatg caccgtaaac attcttctgg gcaacgccct tcttgccgtc tagcatcaca
     1321 ttgtctcatc aacaatggga tggtannnnn nnnnncgggt tacgagcttg gagttgtaaa
     1381 tcggatcctc cagcacgtca cgttttgcga ttttacctct tcttggcatt ttcgcttccc
     1441 tccttcacaa taattcatga attcacaggt actcaatcgg gcggcatccg ttcagcggat
     1501 gttccgccga ccgtagggcc aatggagagg aatacgccgg cattgcagca atgccaatct
     1561 atattcaaat ctccacagtt cccgtggtga tctcagtttt cctgttacgc cggagcagtt
     1621 ttcacgccgc tcctgatttt tggtcagtgt gtaagccctg aaaagcagtt cttacttctt
     1681 gccggccttc ggtctctttg caccgtactt ggagcgagcc tgcattctgt ttgcaacgcc
     1741 ctgtgcatcc agagtaccac gaatgatgtg ataacgcaca ccaggcaggt cctttacacg
     1801 gccgccacga atcagaacaa cgctgtgttc ctgcaggttg tggccgatac ccggaatgta
     1861 agatgttacc tcataaccgt ttgtcagctt aactctggca atctttctca tagcggagtt
     1921 cggcttcttc ggtgtagcag tacgaactgc tgtgcagacg cctctcttct gcggagaact
     1981 ctgatctgtg gtaatcttct tcttggcatt atagcccttc tgcagtgccg gagcggtaga
     2041 cttgtactgc tgtacctttc ttcccttccg aaccaactga ttaaaagtag gcataatttt
     2101 cctcctttgc aaaaaaatag atagattgca gcaggcaaac ctgccgcatc ttttttattt
     2161 tatcacagcc tgtctctact gtcaacactt ttacagaaat tttacatttt tatcgttccg
     2221 gagcgctttc tgctgtctgc ttccctttat ttaatggtat actgactgtc tgtttccgcc
     2281 ggcgcagcac cagcagatac agtaccgcca gatataccag acagccgcct gccgctgccg
     2341 ccgccgcaga gggcagcatt cccagcgcag ccgtctgttc ccggcagaac cacgccgccg
     2401 ccgtacagta ccctgctgca agcacaatgg gcagcagggt tcgcattaga tgcagcggct
     2461 tttccagaca gcgtcgcagc tgccacagga tcagtcccaa cagcaccagg taacacaatg
     2521 atgtggcgat ccctgcgccg gtgatgcaga acgccgggcg tggaatcagc actaggttgc
     2581 ccaggagttt cacgccgacc cctgccagca tacataccac cggaacggcg gctttcccta
     2641 cgccctgcaa aatgctgcaa agcggaaagg tgagacagag gcagaccata ccggggagca
     2701 gcgtgcggag cgcctctgcg gcgatggcag cctcttcggg tctgccggcg tagagaatgt
     2761 gcatcaccgg ttctgccagc gcccacaagc cgcctgccgc cgggagcgcc attgccgccg
     2821 ctgcaccggt taccgaacga atatgcgcca caaccccttc ccgatcctgt tttgcccaga
     2881 gcgccgccgc acagggcagt gcgctcttcc ccagcatatt ggtcacagac ggcaccaggt
     2941 tgaacaccgt gatcgccatg ccggtaaacg aaccgtagac aaacgccgga aaatccggtg
     3001 cagatgcagc tgtcccgaac cgccggagca gttccccgct gtgcagcatc tgcgccctgc
     3061 ccagacagcg catcatggtc accagatcca gcagcgaggt gagattcgtc accagagacc
     3121 ccagcgccac cggaatcatc acatagagca gccgccggaa gatctgcccc atgggtgcag
     3181 ccggtgcatt gccccgtggc aggcggttcc cggagcgcag tccacgcagc agaaagtaca
     3241 gccagcccac ggcggtggac agcgtcannn nnnnnnngcg tcacacccag cactgccccg
     3301 gcagccgcca gcgcagaaag cggcacgtcc gccggcacac ggcggcagac gatttccgga
     3361 tgcgccagca cccactggca cagcgccagt cccagcccca gctttgccac agcctccagc
     3421 gcctgggaga ccgccgtagg cgtcatgttc tgcaatccct cccagtagcc acgttccact
     3481 gccgtcagac agccgaacag cgctgccggc gcaatggcga gcagcgaaaa cagggcatag
     3541 ggctgcgccg caaccagggc ggcgaacggc tctgccagca ggaacataca caggctgagg
     3601 aacagcccca ggacgccgca ggcaaggcgt gccgtatccc ggatacgccg cacctctgcc
     3661 cagtgttcca gcgccgccgc acgggagact gtctgcgcca ccgccgccga cagcccggtg
     3721 accgacaagg catacaccgg cagcagcagc ccgtaggcag tgctgaaata tcccattccc
     3781 gtaccgccca gcacattggt cagcggtatt ttgaaacacg ctcccagcag cttggcgatc
     3841 atggcagatg ccagcagcag cgcagagcct ttccagaacg attgctttcg aaacatagct
     3901 tttctcctgt ccgcataatt ttttgcaccg gatagaaaaa tttatgttgc cgaaaacgaa
     3961 aatatgtgtt ataatagagt ggtaatgccg cccgaggcgg tatggagagc ctgcaaaaag
     4021 gctctaaaga cagcaccgca caaactttgt gcagtgctgc ctgaaaatcc tgtgcagctg
     4081 acggatactg tatccggtca aaacgctgtc tgaaaggaag aatgtaccca tgcaatacaa
     4141 atttgcagaa aatgttgacg cattgcagcc ctctgcgatc cgtgaaattt tgaaattcac
     4201 cagtatgccc ggtgtgatct cttttgccgc cggcagccct gcgccggagg cgtttccggt
     4261 agacaccatc cgggagatct cggcagaact gctccagaag gatccgctcc tnnnnnnnnn
     4321 ntcacagaag gatatccgaa gctccgtgac ctgctgaaac agcggatggc aaagcagaac
     4381 tgtttcgacg agacggcaga cgatctcatc accacctccg gcgcacagca ggcaaacgag
     4441 ctttcctgca aggtgctgct gaaccgtggc gacaccctat tctgcgaggc tcccagcttt
     4501 atcggctcgc tgaatgcgtt ccgctcctac ggcgtagacc tggtgggcgt tcccatggaa
     4561 gaggacggca tggacttagc cgctctggaa gaggcagtca aggcaagcaa aagccccaag
     4621 ctggtctatg tgattcccaa cttccagaac cccaccggca gagtgatgtc tctggaaaag
     4681 cgcaagggtc tgtacgacct ggcgtgcagg tatgacttca tcattctgga ggacaacccc
     4741 tacggcgatc tccggttcgc cggcacagac gttccgtcca tcaagagcct ggacaccgaa
     4801 caccgtgtca tctacaccgg cagcttttcc aagatcctgg cgccgggtct ccgtgtgggc
     4861 tatgtgagcg caccgaagga gatcatctcc aagatcacag tctgcaagca ggtctctgac
     4921 gtccacacca acatctgggc gcaggctgtc tgcgcagcat ttctggagcg caccgacatg
     4981 gacacctatc tggacggtct ccgtgcggta taccgccgga agtgcaatct gatggcagac
     5041 ggcatccgca gccacttctc cggcaaggtc aagtgggaaa tgccccaggg cggtctgttc
     5101 ctgtggtgta ctctgccgga tgactgcgat atgccggcat tctgcaccaa ggcagtcaag
     5161 gactactcca tcgccgtcgt tccgggaaat gcgttcctgg tagacgaaac cgctcccacc
     5221 tcttctttcc gtctcaacta ttccacaccg tccgacgaac tgatcgtcaa gggaatcgag
     5281 atcctgggca agatgacaaa agagatcctg ggcgcataag caaacgaaag gaatttatat
     5341 aaaatggcac agaaaaaaga acacgtacag acacattatc tggtaaccga aaactatctg
     5401 atgacgagag gcgctgcgct gcactttccg gcaaagcagt tctccggcat gatctccgac
     5461 gaacacagcg tttatggcgt ggtcattgat atgcccatcg acccacgtac cctgtccacc
     5521 ctggtggtat acatcaacgg tgcggcaaac ctgtacttca acaacggcgg ttcctacacc
     5581 ggtgcatccc agcggtatca gaccgtggta caggcaggca gactgctggt ggcaaatgca
     5641 ccccgcatcc tgggcgaggc aacaaagacc acttctttcg cactgcccac ctcccagccc
     5701 catcacatct atctgctgac caagagcggc atctacgaaa agaaggtgct gcccacagcg
     5761 atcaaggaag aaagcaccga tatgcaggcg gtttatttcc tctatcagca ggtcatgcgg
     5821 gaactgcgtt ctgcgcagct gaaagaccgt gcatcggctc agaagtaaag gcagcgctct
     5881 atgattccat atgaaaagaa agaacactat accatcgacg acctggcaga gatcgtgaca
     5941 ctgctccgca gtgaagaggg ctgtccctgg gacaaggtgc agacacacca gtccatccgc
     6001 tcagacctca tcgaggagac ctacgaggtc atcgaggcga tcgaccagga cgacccggaa
     6061 atgctccggg aagagctggg tgacctgctg ctccaggtgg tattccacac ccgtatcgaa
     6121 acggaacagg aacacttcac cctggaggat gtctgcacgg atatctgcaa caagctgatc
     6181 gtccgccatc cccacgtatt cagcacggtc caggcagaca ccacggaaga ggtgctgaaa
     6241 aactgggatg ccatcaagga agagaccaag caccagacca cacgcaccca gaccctggag
     6301 gctgtggcaa agaccctgcc ggcgctgatg cgtgcgcaga aggtctgcaa gcgtgcaatg
     6361 aaagaccaca accgctttct gtgcaacgag tctgcactgg caagcgtgac cgagtgcaag
     6421 cacaccctgg aagaggcgat gcacatggaa gacccggaac agatcacccg tgctatgggt
     6481 gaactgctgt tctgctgtgc cggcatggcg cagactctgg ggctttccgc agaacaggtg
     6541 ctgaccgaca ccacgaacca gttcatcagc tgtttccgtg tcatggaaga gacctgcgcc
     6601 gcagaaaatc gtcccatgga gacgctctcc aacggcgagt ggaacgcact ctggaaaaag
     6661 acaagacgca gtctggaaga ggaggactaa gccatgcggc tggacaaata tctgaaagtt
     6721 tcccggctca tcaaacgccg caccgtggca aacgaggcgt gtgatgccgg aaagatcctg
     6781 gtaaacgaca aggccgcaag agcctcttat gacgtgaagc ccggcgatct catcgagatc
     6841 cagatggggc agaagtctct gaaggttcgg gtggaacagg tgtcggaggt cgtccgcaaa
     6901 gaggacgccg ccggactgta tgccgtcgtt tccgagtaaa gcaaaagtca caatacagga
     6961 gcggacagca gatgctgtcc gctttttttg gtcaccatct tcatgaataa aacataagtt
     7021 ttcagaaaat atgtttctgc ttttgacttt ttatttttac gcagaaagcg catctccatc
     7081 tgcgaaaact gcaaaatgct cattttatgc aaacagctgt tcgctttcca agggcatatt
     7141 ttttcttatg tatctataag aaaaacaagc tgtttcacca tttttcgcac ctttggcgca
     7201 tttgggcaaa gtgccacgaa atccactgag gaaattgcat aaaatgcacg aagttttcat
     7261 ccgaatcttg gtacaatagt acattgacag tttgctacaa aaaatgatat aattcgaata
     7321 tagaatttgt gtgcttgagg gggcatacag ttccatccga ccctgcgaaa acagtgaggg
     7381 tgcattcgtg aaaaaatacg gacggcaaag aatgcggctg tccacgatgg agggtaagtt
     7441 tatggagaat ttgggctacc tgctgcagga tgacgacatc ccgcaggctg tgaaacggcg
     7501 cagcgtaaaa gaaaaatcca aatccgttca gctggagaat gtatcgaaaa agtatttatt
     7561 tgccaagcgt gccattgacg tgattctgtc gttctgtgca gttgctgtac tgagcgtgcc
     7621 gatgctgctg atcgctctgg tcgtcttttt gcaggacttc cacaatccga tcttcaaaca
     7681 ggttcgtgtc acaaaggacg gacggttctt caccatgtat aaattccgca ccatgtgcgt
     7741 aaacgcagag caaaagctgg aagagctgcg ctcgcagaac gaggctgacg gtccggtctt
     7801 taagatgaag aaagatcccc gtgtcacacc catcggcgga ttcctgcgaa agacaagcct
     7861 ggacgagctg ccccagtttt tcaacgtact gaacggctcc atgagcctgg tcggtccccg
     7921 tccgcccctt ccgaaagaag tggaaaagta caccccgtac cagatgcacc gtctggatgc
     7981 caaaggcggc atcacctgct actggcagtg ttccggcaga agcaacatta tgtttgacga
     8041 gtgggttgag ctggatctga agtacatccg tgaccgctct atctccaccg atgcaaagat
     8101 cctggcaaag actgcagttg cagttctgcg cagagacggc gctgagtaat tttctcgcat
     8161 atgatatagc aaatataaca acgcccggca tgaccgaaaa gctgtcatgc cgggcgtttt
     8221 ggttttggtt ttaattgtta caggttgccg gtgcggtgca tggcaatggc gatcgccgcc
     8281 agaggtgcag tctcacagcg gagaatgcgg tgtcccagcc agaccggaac agcgccgttc
     8341 tgcttgagct gttctgcctc ttccgggtca aagccgcctt cactgcccac caccagggca
     8401 agggtgcggc agtccgtcag attgacctcg ctgaaccgga ctccgccgcc ttcatacagc
     8461 agcagtgtcc ggtctgcctg ctgcatctct gcacacgcct gggcaaaggt gagcataggt
     8521 gcaacttcgg gaatgatgcc acgccctgcc tgttttgccg cagacgctgc gatcttttgc
     8581 agccgtggca gctttttctg gaactccgcc gccttgggac gggagataca gcgcctggtc
     8641 agcacgggaa cgatacgcac agcgcccagt tccacggact tctggatgat ctgctccagc
     8701 ttgtccgcct tcggcacagc ctggtacagc gtcagcgcca cggtaggttc tgcggcgcac
     8761 agctccgaag agaccacatc cagatggact tcctccggcg taatggagcg tatcctgcac
     8821 tgatagtccc gtccgtaagc acacaccgtc agcatctctc ccacacgcat ccgcagggaa
     8881 agcccgatgt gccgggcatc cggtccggtg atgcagacat ccgattcgtc tagttcttct
     8941 aaaaaaaatc ttggcataac gatttccctt tcttttgaca ggttggtgca gcggtttgcg
     9001 ctggacacgg cacatcaatt tcgcacaaat caattttaag aaagtccccc ttgaaaaggg
     9061 ggatgtcaac gcaagctgac agggggataa tccctccgaa tactttttac gaaacgtccg
     9121 ccccgtggca attctccctt tttaggagga atttttgatg cttgatttga acagcaattt
     9181 cacattctca gttgacaacc gccggaaaat gtgatagagt ataaggtagc atatacattg
     9241 gaaaggaact gatacctcat gacatataga acccataccc tccggcgaat tctgacctgt
     9301 gccgctgccg ccgcactgct ctgcaccagc tttgccggat gttccggcga ttcttcttcc
     9361 agcagcgaat ccggcatcaa cgtggacatg gaagacatgg aatacggcgc aaccatgcgt
     9421 gacgacacca gccgtgccgt accgctggaa tacgacaagc gctttctgga ggacggcgaa
     9481 ctggacgctc tggcgaacta ctacggttcc atccagaatg aagatgtagc actgtttcag
     9541 tcctgcaccg tttcggaata catggaatcc ctttacaaaa atgcctacgg cgacctgctg
     9601 gacgacagcg cctatgtgtc ccagcagaag aaaagctatg aagatcagat gggcggcgat
     9661 ttcacgtttt cccagatcat ggtcaatggc tgtctgaaac aggacgagac cggctctaac
     9721 gcagaatacc tcaccggtat gctggacgag ctgaacggcg acagcaacta ctgtgcagaa
     9781 cacatggaaa gctgcaagac cctcaccgta cagccggtca tcaccaacgg cacagacacc
     9841 acctattgtg acgaagtcac cgtattcatc atcgaactgg acaacaaata ttacgtttgt
     9901 gcgtgagaaa ccgcactgca aaagcatccg tccggcgcag tctgttagga ttcagactgc
     9961 gctttttttg tctctggtgt ttcctccgtc tctgtttccg gcacttcttc cggttcgggc
    10021 gcattcggct tgcggtagat cgcagtgatg ccctctgtct gttgattttg tgcttcggca
    10081 agtttttctg ccgcctcttt ttcctggcac tcccgcatct tgtccgaaag ctgatccatc
    10141 gcccggcgat agcgccagaa cagcaggatc atggcgatca gatacacgac ggtca
//
LOCUS       DAWENO010000071        10182 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2783_length_10182_cov_13.197986, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000071 DAWENO010000000
VERSION     DAWENO010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_10182_cov_13.197986"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2546 a         2487 c         2879 g         2270 t
ORIGIN      
        1 aaaacttttt gaaaaagtgc aacatttcgg gctgctcgat tgtatccata gtgaagggat
       61 ttttagcggc gtgaagaacg ccgccgtatc ggaaatgaaa aggtgtgttt tttttatcgt
      121 gagatgtttc ggtttttcag gcggcacaga gaccgggcgg aaacaggcgc tgaaagggaa
      181 acactgatga aatggaatgc gccgtatgcg gcgatgttgc acagaaagga acggcagttc
      241 aatttcacca gtgcttccgc acgacgggag agtgcattgg aatctcctac ttttccattt
      301 cctctgggat gtgttttcac agatgttttg tggaaacaca ctccgatttg acagtgcgta
      361 tcagcgcttt ggggcgctgc cttgcagccg ctgtcaaacg gcatccggca gacagtattt
      421 tactgctgtt tgcattctat acatactttg tttatgttta tgctgtatga taaaaggtag
      481 aaagacagca tgagcagcaa aacagcgtag gacagaaaat cggcttgcgc tgcggaaaaa
      541 aattgccgag gtgcagacga tagggggcgt ttccgtgccg caggatgaaa ccacttttgc
      601 gcagttttat gcacggcagg tcaatacggt atatcggata tgctatgtaa ggctgaaaaa
      661 tcagatggat gcagaggacg tgacgcagga ggtattcctg cgggcgatgc agcagccgca
      721 gctttgggga gacgatgcac acgcaaaggc gtggctgatt gtgacggcat caaatctctg
      781 taagaatgcg gtgcagcact ggtcacgttc caagcgggaa gaagttgaag attgggaggc
      841 ctttctcgga gcgacagaac cgcccgagga agagtccgct gtgatgacag cggtgatgca
      901 gctgccggat ccgtatcgga ccgttgtgta tctattttat tttgagggat attccggcat
      961 ggaaattgcc ggaatgctgg gaaaaaagga atctacagtc cgatcgctgc tgcatcgggc
     1021 gaaaaaacga ctgtccaagg tgttgggagg tgagccgtat gcgaaatcag gaagatgaaa
     1081 tgctgaaaaa gtgtttgcgt cagactatgg aacagatcca gccggatgaa gagacgaaaa
     1141 aacggatgta tctggagatc atgcggcggg catctgcggc agaaaatgcg aaaactgctg
     1201 cgcagaagaa acgcccctgg tatctgcgct ggcagaccag taccggcatc tgtacggcgg
     1261 cgctgctctg cgtggtgatc gctgcggcat ccatccacag caagatgccg gacggcacca
     1321 accagctgga ggtcgttccg ccggctgcgg tcacgactgc ggttgaaccg gaggaacgcc
     1381 cggacactga gacagaacct gcggcgaccg cagaaaccac tctgccggaa acggcatcgg
     1441 atgcagatgc ggcggtgcag agcagtgcga acacagagat gaaaaccatt gcagaagaac
     1501 cgggagatat tccggaagtg atagaaggac ctgcggacaa cacagcgccg cagactacag
     1561 gaaagcacaa tgcacctgcg gtaacgactg ctgtgccgca gaccagcaag ccggcggaga
     1621 cggcccgtgc agcggagact tcgaagaaag cggacattgt gacaacggct gcaccggtgg
     1681 tgacaacact gccggagacc tcaaaggagc aggaaacgga gacagttccc atccggcaga
     1741 acatttacct gtactataag ctgatctggc gcaacaatcc gtatgacacc aactatgatg
     1801 aagttcccgg cggagtgctg gataactatc tgggctatgg tgtgacccag ggcagcgatg
     1861 tcgatgacac atacacggtg ctgatctatt ccatcacagg cgttgacccc agacagcagg
     1921 tagctgtcca gtatgtgggc gaagaaaagt attatgtgtt taccatacag taaaagtgtg
     1981 ttttggggaa agtgaaaaaa tgaaatctcg ttatgatgcc acgatatctg ccgtggcatc
     2041 attttttatg gaaatcgctt gacaaaaaaa cgaaaaaaca gtatactaaa atagaatgaa
     2101 acgaggtgcg gaatgcacaa aaatttgcag ttctggaggt attgaattgg caaccaaaaa
     2161 aatcaaacag ctggaaaaga tcctgatcgc tgtggggatc gctcttctgg cagtggtaat
     2221 catctgcatt gtggtgttca gccgtgcgga caaggtctct gcgccggagg cgattccggt
     2281 actgactacc actacgacaa ccaccacgac caccaccgtt accacgacca ccacggtgac
     2341 ctatgactac accatgcaca tcgacatgga aaaggtgaga cagcaccacg aaaagaatcc
     2401 ggatgtggtc ggctgggtct atgtaaatga tacacccatt gactatccca ttgtgcagaa
     2461 ggacgacaat gcctactata ttgaccgtga ctgggagggc aatcacagtt ctgccggcag
     2521 tatcttcgag gactttcgtg gcaagatcaa cgagacggaa aacacgctgc tctacggtca
     2581 caacatgggc aacggctcca tgttccactc tgtcaagagc tttaaggaag aggaatgggg
     2641 cagacagcac atctactttg aagtggcaac gctggaaaag cgctatctct accgcatcgt
     2701 ggcatcgtcc gtgctgaacg gcgaggacgg cgcagcgttt gattactgga accgcatcac
     2761 actgaaccag gaagagttcc agaagtacat cgaggaaata cgctccacgg cgcttgtctg
     2821 gtatgcgccg gacgatgatc cgccgaccta cggcgataaa atgatcgccc tgcaaacgtg
     2881 taattccggc gcagacgacg gcattcgctg cattctgttc ggacagtgtc tgggtgagta
     2941 ttaagcgcat cacatcgaca aacataaata aaaagggcat cctgtggaaa caggatgccc
     3001 tttgctttta gcaattcatt agaacccatt cagatgatac tttgtcatca gatcgggata
     3061 gcatttatag ctgtagtcca ggtctacgtt gccgttgatg ccgttgatgt tgccggagtt
     3121 ggaatactgc cacataccgt agcttgtctt ggaataggac gggcggctga cattgaagtg
     3181 tgcgacccag atgtcatagt cctccagaac cgactgataa acataggtgt tcaaaaagct
     3241 tgcgtagctg tacagggaaa cgtaatagcc cttggattcc atgacctcgc agaatgcgtt
     3301 gataacggcg gagacctgtt ccttggtcag tgcggtctgc accttgtcct caatgtccag
     3361 aaccaccgga tactcaaact ggtatccctg gatgacgctg gcgaaaagct ctgcctcctg
     3421 ccttgcctca gccggggtag tggcgtaact gtaccagtat gtaccgcagg caatgccggc
     3481 ggccttggca tttttcatgt tctgctcaaa gtaagggtct ttctggttgg cgaccttgcc
     3541 gtagcctgca cggatcatcg caaagtcgat gccgtcagcc tttaccgcct tccagtcgat
     3601 gttggtctga tacttggaaa cgtccacgcc acggtaagaa tccttttgcg cccatgcgcc
     3661 gccacggttc gttgtggtgg tagtcgttgt cgtggtggtg gctgcggtcg tagttgtcgt
     3721 ggtggtggct gcggtcgtag ttgtcgtggt ggtcgtagta gttgttgtcg tagttgttgt
     3781 ctccgttgtt ggcgcagcag ttgtcacggt cgtgtctgcg gtagttaccg ctgtgctgtc
     3841 ggaggagaca gtggttgccg gagcggcagt ggtcgtggtc tcttccgttg taaagaccgt
     3901 cgtttccagc ggtgtggtct ctgttgtgac agagccgttt gcggtatccc attcactcag
     3961 aaatgtgatg gaatcctctg tgtaatcgcc ggtagcaccc tgataagcgc accagtacat
     4021 cagcaggaaa atgtcggtgg agtcgattct tccggagtga tccacatcca ggagcgtctg
     4081 attcttctcg gaaaaggtgg acgcatagcc ggcgccgaaa taggcgcagt ccagcagtgt
     4141 ggcatagctg agcgtcagaa aatcgcctcg ttcggcatcc tgtaccagct gttccggtga
     4201 cttcaccaga ttcgggatcg tttcggaaat gtcggcatct gccgtgttgt taccggcagc
     4261 ggaaacgctg ccaaagggca gacaggatgc gcagagaatc gctgcggtga acatactgcc
     4321 cagcttttga agaacgtgat ttgccatcgt gaaagcctcc ttgcggtttg ctttttatga
     4381 ttactagata aagtacagat aaatgagccc cgccgcctgc aaaactgcca gcaggggaaa
     4441 gaagaaccgg aaataccagt gccgggtctt gtgccggaag aaaaacattc cggctgtgcc
     4501 gccgatgccg ccgcccagga tcactgccag aaacaaggtc ttttccggga tgcgccacag
     4561 gtgctttttg gcacgctgct tgtcgatgcc cataagggcg aatgcgacca gattgactgc
     4621 ggcaaaataa cccagctcca gatacagctg caccattatg cctgcccttt ctggaaacgg
     4681 agctgacggg gcagcgcagc cgcaatggca tcctgcacgg cgtcgttggg agtgaagatg
     4741 atgcgtgttg caccgtattc ctctgtctcc aggtcggcgt aatattcgcc ctcgccggtg
     4801 ttgtcggcac aaagcacaac ggttcgttcc gtgtcgtccg ggatatcgtc ggtcatttcg
     4861 ccgtaagcgt caaactttcc cacatccacc gtgatgaggt gtacccgttt ccggcgtgca
     4921 atgatcttgt ccacatccat ttctccgttg gtgacggcat attcatattc caccgatgtg
     4981 ccggagatga ggtagtatgc accatagata atggcacagg tgatgaccag gataaacggg
     5041 atgtgtgtca caaaaaccag aaatacagat gcagcgcaga gcagcactgc aagcaggatg
     5101 atcccagcct ttttggcatt gtcctgtgaa tccggacgtt ttttcaccat gtattccgca
     5161 aaattgtcca tatgcgtaaa actccttttt cgaacaagcc ggattttcgg tctccgcctg
     5221 ttcttctgtc gttctgctga ggatgccccg aaaagaaaac tgtaccgtct tgcagtaaaa
     5281 attggaattt gcccatcttt tttgtgcaag ttgctgaaaa catttctctt tttgcatcac
     5341 ccaatttcta ttatagcata tgccgcaaaa aatttcaaga aaaaattttc aaaaggactt
     5401 gatttttttt gtaaaagcag gtataatgtg atttgaaaac acagtgacga agcgagtagg
     5461 catagactga ggcgtccgca tgacagaaaa aatgcggaaa ttgcggtggg aatcccatcg
     5521 ccgccgaaaa ggagccgccg gtacagagaa cggggtgcgc tgagaacccg tccggcggaa
     5581 tatgctgaag ttcccttccg agtggctggg ctgaacccgg aaaagtgtac ttgcagtgca
     5641 aacatttttc cagaattagg cttggatgtg gcgttcgcat taaggacaga agagtgacgg
     5701 ctctttgaaa atgggtggtt tgaacaagca tttgccttgt cctgttttcg caggacaggg
     5761 ctttttttat gaagtgtccc atagagccaa atgaccgcag gaaagcggag gaaaggaacg
     5821 atattatgaa agaacaactg gaagcgatcc gtgccgctgc caagcaggca ctttcggatt
     5881 gtgcagattc caagctgttg gagacactgc gtgtgcagta tctgggcaaa aaaggtgaac
     5941 tgaccggcat cctcaagcag atgggcaagc tttctgccga agagcgcccg gcaatgggtc
     6001 agctggcaaa ccaggtgcgt gccgatattg agaatgccct ggcggaaaag cagaaaaagc
     6061 tggcagatgc aatgctggcg cacaagctgg aatccgagac gctggacatc acgctccccg
     6121 gcaaaaagca gaaggtgggc agtatgcacc cactgaccat cgtggaaaac gagatccggg
     6181 agatcttcct gggcatgggc ttttccgtgg cagatggtcc ggaggtcgaa tacgattact
     6241 acaactttga ggcactgaac ctgccgccgg atcacccggc acgggacacc caggatacct
     6301 tttatattac agacaacatt ctgctgcgta cccagacctc ttcggtacag gtacacgtga
     6361 tggaaaacca gaagccgccg atccgtgtga tctctccggg acgggtttac cgttcggatg
     6421 ctgtggatgc cacacactct ccgctgttcc atcagatcga gggactggtc gtagacaagg
     6481 gcatcaccat ggcagatctg aagggtactc tggaactgct gatgcagcgt ctgtacggcg
     6541 atgactgcaa gatccgtctg cgtccccacc acttcccgtt cacagaaccc agcgcagagg
     6601 tcgacgttat gtgcttccag tgccacggaa agggctgccg gatgtgcaag ggcgaaggct
     6661 atatcgaact gctgggtgca ggcatggtcc acccgaaggt gctggagggc tgcggcattg
     6721 acagcagcgt atattccggc tttgcctttg gtctgggtct ggagcgtatc gcaatgcgcc
     6781 gctataatat cggcgatatg cgtctgctct atgagaacga ctatcgattc ctggaacagt
     6841 tctgagtggg aggtaagaga cgatgaattt atctatgaaa tggctggctg attatgtgga
     6901 ctgcggcgtt tccgtcaagg aattctgcgc cggcatgacc atgagcggct ctaaggtaga
     6961 aacttatgaa acagaaggcg acgctgtcaa gaatgtcatt gtcggcaagc tggtttccat
     7021 tacgccccac gaaaactccg accatctcca ggtctgccag gtagacgttg gcggcgatgc
     7081 accgatccag atcgtgaccg gcgcacagaa tattgtggag ggcgcactgg tgccggtggc
     7141 gatgatcggc gcagatctgc ccggcggtgt acatattaaa aagggaaagc tccgtggcgt
     7201 agagagcaac gggatgctct gctctctggg tgaactgggt ctgaccaagc acgattttcc
     7261 ctatgccatc gaggacggca tcttcctcat tgaagaggac tgcaagccgg gacaggacat
     7321 ccatgaggcg atcggtctga atgatacctc tgtggagttc gagatcacct ccaaccgtcc
     7381 ggactgtctg tcggtagtag gtctggcgag agaggctgcc gtaacattcg gcaagcctct
     7441 gaatctgaaa gcgccggaat tccacggctc tgaggacaag ctgagcgaca gcctgtctgt
     7501 ggcagtggaa aatgcacagc tctgccctcg ctatattgcc ggcatggtaa agaacgtgaa
     7561 gatcggtccg tctccccgct ggatgcggga acggctccgt gccagcggcg tgcgccccat
     7621 caacaacctg gtggatatca ccaactatgt gatgctggaa tatggtcagc ctatgcacgc
     7681 atttgaccag cgttacgtca aggacggcaa gatcgttgtc cgcaatgcca aggacggcga
     7741 gaccatcact acactggatg gacaggagcg ccagctttct ccggaaatgc ttatcattgc
     7801 cgatgcagaa aagcctatcg ctgttgcagg cgttatgggc ggcgaataca gcggcatcat
     7861 ggacgacacc aataccgtta ttttcgagtc cgcttacttt gaaccggtac aggttcgccg
     7921 cactgcaaag aaactgggaa tgcgtactga cgcatcggca cggtatgaaa agggtctgga
     7981 tccggacggc tgccagcgca cactgaaacg tgccatggaa ctggtagaac tgctgggcgc
     8041 aggcgaaccg gtggcagagt tcattgaagt ggacaaccgc accgcaaagc cggcagagat
     8101 cccctttgat ccggattgga tcaaccgttt cctgggaaca gagatctccc gggaagagat
     8161 ggtaaagaca ctggaatccc tggatatcca ggtgaaaggc gatgtctgcg tatcgcccag
     8221 tttccggatc gacctggagc gcccggcgga tatcgcagag gaagttgcaa gaatctacgg
     8281 ctacaacaac atcccgtcca cagtcatccg tggcgtggct gaggcacagc tgacaccgca
     8341 gcagcagttc ctgcggaagg cagaacagac catggtaggt ctgggatact acggcacact
     8401 gacctattcg ttcacttcgc cgaagtgctt tgaccgcatc ggtctcccgg cagacagcaa
     8461 gctgcgcaag accatcacca tcaccaatcc gctgggtgag gacaccagca tcatgcggac
     8521 gaccacactg ccgtgtatgc tggatgtgct ggcaaccaac tacaagaacc ggaatgctgc
     8581 agtggcactg tatgagatcg gcaaggaata tctgcccacc ggtccggaca cactgcccac
     8641 cgagccgaac cgtctgacca ttggtatgta cggcggcgat gcagatttct ttgcactgaa
     8701 gggcactgtg gatgcgctgc tgaaggaact gcgtctgccg aaatgcacct atgtgcgtcc
     8761 gtctgaggca gagggtgtct ttgaggaatg ctgtgcgctc catccgggca gaagtgcagt
     8821 gatcctcagc ggcgaaacac ccatcggcta cctgggcgag ctgcacccga ccgtacagaa
     8881 aacctacggc atcggcacca gaacctatgt ggcaaagctg ctggtacagg aaatggctga
     8941 gatggcagag gcagaagtga cctatcgtcc gctgccgaag ttcccggcaa tcacccgtga
     9001 cctgagcctg ctgtgcgatg atgcactgcc ggttggcaag atggaagagg caatcgaaca
     9061 ggctgccggc aagctgctgg aagaagtgac tctgttcgac gtatacaagg gcgaccagat
     9121 cgctgccggc aagaagagcg tttcgttctc tctgcggctg cgctctcacg agggtacgct
     9181 gaccgatgag caggcagatg cagccatgaa acgtgtgctg aaagcactgg ctgccatgga
     9241 tgctgtactg cgtgaggcgt aagcgccgtg tactgtatct gaggtgattc tcatgaaaga
     9301 aaaaaccgaa aagcagccaa aaccgaaaaa ggaaaaacgg gaacgcacac cacgtgaggc
     9361 aacggcattg atgctggggc tggtttccct gggacttgcc gcagcaggca tcgcatcgct
     9421 gatgtgggtg tctgttctgg tggcagcagt cctgggcggc tgtgctgttc taggcggatt
     9481 cgccagcctg atggtgggaa aaggcggaac gctcccggca atcgttgccg caatcgccgg
     9541 ctttctgacg gtgctgtctg cgatcatttc tatgaaaatg cagtgatttt gctctgatat
     9601 aacgaaaaaa gcggagaagt cgtgagactt ctccgctttt tgtatgtcgt ctgaaaatta
     9661 agacaggttg gaaagctgat ccagaatgcc gcttccagcc agaatcaccg cattgacgat
     9721 ccacataacg atgctgatga caatgccgat gatgcccagg atcatgccgg ctttctgctt
     9781 gccgtcagtg gaaccctctt tctttgccag cactgccaga acgatggcaa tgatgccgca
     9841 gatgatgccg gcgatcggaa tgcagatgcc aatcagcgag ataatgccca gaaccagggc
     9901 agcagttgct tttccgtttt tctgctccat ttttttacac cccctatttc ccgatatcat
     9961 ttgacaaatc acacgtaaaa aatatggaag tgtcaaacaa atttattata ccatagaaat
    10021 atgcaaatat caagtaaata aattgtaaat tcaaaattcg agaaaatttg atattgtgtt
    10081 ggatttcacc ttgcttatcg gtaatgtttc tgaacatttg acaactacag gtatctgtgt
    10141 tatcataaag tcatgcaggc gttgtgcagt gatgtgttat ca
//
LOCUS       DAWENO010000072        10071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2809_length_10071_cov_11.585064, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000072 DAWENO010000000
VERSION     DAWENO010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_10071_cov_11.585064"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3263 a         1726 c         2439 g         2643 t
ORIGIN      
        1 tctgaaccag acacagactt ataatcagag acagttcccg tatttgatcc ggctcacgac
       61 aaatgagcgg attgaaatca acaagcctgt ttttcgtttg ggaaaagaaa gaaattatgt
      121 ggattatttc atttcgaaca acggtgcagt cagcagaagt catgctgata tcatcaccaa
      181 aagcggacat tattatgtga tggatctgca cacgacaaac tttacataca tcaacaattc
      241 catggtaccg gaaaatacgg aagtggaaat ctttgacggg aatatgttaa aactggcaaa
      301 tgaagaattt gaatttcacg tgcaataagg ggtgaagcta ctgtgcatta cagagtgacg
      361 gcagatacgg atatcggtac cgtaaaagca acgaatcaag aaagtatttg tgtatttcat
      421 gcagattcgg ttcgtggaga aattgtaatg gcaattgttt gcgacggaat gggcggattg
      481 gcgaaaggag aacttgcaag cgcaacggta attcgtgctt tcgaaaaatg gtttcatgat
      541 gaattgaaac acgaactgta tcaactggat atgcgtgtga ttgcaaacaa atgggaattg
      601 ctgctgaagg aattgaacct gaaaatcatg gagtatggca aacagtatcg tgtgaatttg
      661 ggaacaacct ttacaggaat gctgattgtc ggctcagcgt attttgcggt acatattgga
      721 gacacccggt tgtatcatat tgatcgagat attgtacagc ttaccgaaga tcatactgta
      781 gttgcacaga agataaaacg aggagaaatt acgccggatc aggcggagag tgatttacat
      841 agaaatgtgt tgacacagtg cgtgggagca tcaaacaaaa ttgttccaca agttttgttt
      901 ggaaatgcgg atcaaggtgt gtatatgatt tgctcagatg gatttcgtca taagatttct
      961 gagcaggaga tccgcaatgc ttttcaaaag gatctgctgt ctgaacagga tgcaatgcac
     1021 gcacaggcaa agcggatgat ctcgttggta aaacagagag aggaacacga taatatttct
     1081 gttattttga tctgtgcgga atgaaaaagg agtgtgtttc atgttttgtc caaaatgcgg
     1141 agcaaatatg cgtgatgggc agtattgccc tgtttgcggc tactttgctt cgggtcaagc
     1201 tccttcggga aattattatg gaagcgcaaa caacaaagca gctgtttctc ccaaaaatag
     1261 tcggaataaa attttgccgc ttttggcaat tctgattgct gtagttttgg cagcagcgat
     1321 tttggttcgg aatatgacga acaatggaac gcacgcaatt aaaaagttct ttcatgcaat
     1381 ggaaagacaa aatgcaagag aaatgatgtc atgtgttccg gatgatttta tcgatgatct
     1441 gattgatgac tatgatgtga aaaaaagcga tattaaagat gcgctggaga aatatctgaa
     1501 aacagattgg gtcagtgatt atgtggagac ggattatgag ccaggcgaga aagtaccttt
     1561 gaaaatactg gatcatgaaa aaatggataa ggaagaatta tcagatatcc agcgtgatct
     1621 gacagatgtc tggacgctgt cgtgcaagca gtttgactca aagaaaatca aaaaagcgga
     1681 gacgtacaat attgattttg ataatgacaa tagttatttt tatatcgttt ctgcttttca
     1741 atataaagga aattggtact attataatgc gctggcgctt gtggaacacg caaccagatt
     1801 gtatggatga tcttattttt tgaggggtga atcgtcttga ttaaaatcca tgatattgtc
     1861 gatggaaaat atgagattct gaaagaaatc ggtcatggcg gaatgtctac ggtttatttg
     1921 gcaatggaca agaatctgaa taaacagtgg gcagtcaagg aaatccgaaa gcagggaaac
     1981 aataaaattg acggggctgt tgtaaacagc ttacaggtcg aagcgaatct gatgaagcga
     2041 ttggatcacc ctgcacttcc gagaattgta gacatcataa acagcgatga gacgatctgc
     2101 attatcatgg actacatcga aggagaaaca ctggagaaga ttctcgaaag atatggcgca
     2161 cagccggaag atgtggttgt ggaatgggcg atgcagctgt gcgacgcctt gatgtatctg
     2221 cattcgcaga atccgccaat tatttatcgg gatatgaaac cgggcaatat tatgttgaaa
     2281 ccggagggaa atctgaaggt tttcgatttt ggtattgcca gagaatataa ggaaaaaagt
     2341 cttgcagata ccacgatcct tggaacgaaa ggctattgtc ctccggagca atacggaagt
     2401 cgtcaaacag atgctcgttc cgatatctat gctctgggta tgacgatgca ccatctgctg
     2461 accggaaaag atcctcgtgt tgccggatat gagtatgcct caattcgcca gtatcgtccg
     2521 gaattatccg gaagcctgga gcgaatcgta gacaaatgta cagcgttaga tccggaaaaa
     2581 cggtatcaaa gctgttcaga actgatgtat gcgcttcaaa atcgagaaca tgatgaagaa
     2641 aaatacatca aaaaacaaaa aagaaagctg ggttcattta ttgcactttc tgcaatgaca
     2701 gttgtttttg cgatatcagg tacagtgctg cattttactg cgtctagtct gaatcgcagc
     2761 aattacgaaa atctgatcaa tctttctgaa tccacttcct actctgagaa gataacaagc
     2821 tatgaggatg caattgcgat ttatcccaat gacacaagag catacctgaa aatgctggaa
     2881 gcttatgagt atgaaggaac attcggaaaa gaacaaaatg atgaatttct ggcgctgtac
     2941 aataaaaacc aatccgggtt tgagtctaac agcagtgatt ttgcggagtt aaactacaag
     3001 atcggaatga tgtatttcaa ttattatatt tctgagagcg gtgaagtgca gttttcagaa
     3061 cgtgtgcaga aggcgtatcc ttttttcaaa gacaattacg agaaggcgcc agatgatttt
     3121 gcagaaaaaa agatctcaga ctgttattat cagatctgtc agttctataa aaagtatatt
     3181 ctggcaaata ccaatgttga agaagcggca aaatcagact atgaaacatt gttgggtaca
     3241 atcgaaagtt ctttggatga ggtaaaggat gcaggcgctt acgatcagct gacgctctat
     3301 aatggaatta tcatgctgct gtatgaccag cgtgcgaatc tgaaaagctc gaatgtggaa
     3361 aaagacagga tcctgacact catggattcg atttatgata aaacccaaag tctttctgta
     3421 aataaggagc aatccaagaa actgcaggga gaaatcgatg attactatga cacatatcga
     3481 aaagcagtag aacgaactta ttccaataca gaaattacaa aggatcaggc ggaaacggag
     3541 ggagaatcaa atggttgaga tcattaaaac tctatcgtgg atttcatttg ccgcagccgc
     3601 tgtgtgcctg ctgcttgcag tattcgtctg gtttcggttt cagattatgt ccgtcatcaa
     3661 cgatttgact ggaaaaaccg ccaagaaagc aattgaacag attcgcagta agaatgtcaa
     3721 aagcggggac aagtcctatc gttccagtcc ggtgaatata aatcgtggac ctttgacaga
     3781 accggtaacc aatgcagctc ctgtgaaaac agaaaagttg tcgggagatc tggaaacgac
     3841 actgctgcgg gaagataaca aaactgtgat cttgaatgca gatggtacta ccgtcttaaa
     3901 tcatggctct gagcaggcag cggcagttcc aaaccgacct gaagtagaag ttcaaatgat
     3961 agaacaggtt gtatttatcc atacaaacga ggtgatttca tgaaaagaca ggcaaaaaac
     4021 cgaattgttc tgcttttggt gatagctggc tgtttgcttt gttcttctgc ctgtgtgctg
     4081 tttgttccga tgatacataa ccacagtgca tggacaatat tatgttcctc tgcattttgg
     4141 ggattcctca tcattggata cggacttctc tggatgataa actggaaacg cagaaaacag
     4201 atgaaaaaag gtgaaaagcg gcgaattgga atcataacat ttttccggaa tcctcctgca
     4261 aagatgatgg atttcatttg tctggctgga attctgatat tgattatctc ttcattcgct
     4321 gcaccgctgc aaggctattt gcagtatgtt ttatcttttc ttgttatttt ttcatttcat
     4381 atgcactgca tattgaatgg agaaattttt cgatatatgc aaactgagat aaggagttgt
     4441 ttggaatgaa agaaaaaaag tttttcatgc gtatcgccgc tgcggtgtca tctgcggtag
     4501 ttatgttggc aagcgtgccg gcaggttatg caatggtttc gtttgcggaa gaaacttctg
     4561 cagccggaac ggaaagcagt gtttctgaaa cgacagatga tgcagcacag caggaaacgg
     4621 tgacgctgag cggttatgta attgctggag atactgtggt tgccaatgcc gatgtgtggt
     4681 ttgatgacac acacagctgt aagactgaca atgacggaaa atatactttg gaaaatattc
     4741 cgaaggcaga ttcatatcag gttactgtga gtcgtggctc cggctatgcg gatgaaactg
     4801 tgacaattgc ggaagatggt tctattcgtg ttaccccgta ttacacagtt tcttttccga
     4861 atgatggcga acatatcttt tatatagtag atgaaaataa tcaggaacgc caggcagata
     4921 acgttgtttt gcaagccggt tctgttttat atttcagagt ggagaatctg acggcaaatg
     4981 agcagctgtt gaattgtgat aaggatattt ttgaaataga aggtctttcg agtaaatatc
     5041 ataaagttga agtgaacagc gatattgata ttgagattga agtttctacc ggtgatgctg
     5101 tatttcagta tgatttggaa aagcacgtta tcacatatca gctgccgtcc tcagcgaaag
     5161 aattatacta tgcaaatgtt ccgtcaaatg aaaaccaaaa agtaactgct gattatgtca
     5221 gagaaaacgg atcttgcata gcatcaggtc agcagagcgg tgaaattagc aagttgacga
     5281 gtggatacta ttacttctgt tatgtgacag cagacggtat tgtttcagat gtttttccgc
     5341 agccgaatgc gctggaagtt gatgtggatg cacctaaggt ttctgtggat gcaattgtaa
     5401 ctgtaaatgg aaaatcagaa attacattta cagtcaagga tgagggcaat gaaggcgatt
     5461 ttgtaagcgg aatcgataag gtagggctga ccaagacagg atctgccgac aatatcatag
     5521 aagccacaaa agtggatgaa agcggtacat attttgctgt ttgcaatgag tctatggatg
     5581 gttatcagat tgcagcggtg gataaatata aaaatgcagt gcttgaaccg atcagcggct
     5641 ttacaatgga agtcacatat gatacaaaaa atttggagca tgacaaaaaa gtgaatgtaa
     5701 agacttctgc cgactgtact gtgacttgtg aaggtgctga aaagaccaat aacgacaagg
     5761 aattcctgat aaaggaacag ggaaattata caatccaggc tgtcgatgaa aacggaaagc
     5821 ttcttgcaaa aacgacaatt caagttggaa atatcgataa taagccgcct gagatttctg
     5881 gactttcaaa tattggatgg acgcatacaa gcaacaattc tgtgaaaatc atgggaaatg
     5941 tatcggatga tagtcataat atcgctgaaa tcagaattga gtatgcgaaa gtcgagggaa
     6001 gtgaagaact ttccggagat gccctttaca gtgaagatgg tgttaatatt gaagcggatg
     6061 gcagattcgc gattaccttg ccggatgaag aattcaatgg cacctattat attcgtgcaa
     6121 tagacggatg caaaaatgtg tcttctcctg tggctgttga agtgaagata gacaatactg
     6181 ctccgcagaa cgtttccatg tcttatgagc tggaaaaaaa taaaggattc atcaaagata
     6241 tagcgaatgg acttagcttt ggactggttt tcaaggataa aatcaccatt gaggttgagg
     6301 catccgatga acgtgaaaaa gaagacggaa caaaaataga cagtggtatc cagaagtacg
     6361 agtataagtt ggtacccgag aacacttcct ttgaggatag agaatggaaa tcagagactg
     6421 aaagcacaat tgtaattcct gccacagact atccagaagg ttttcaagga aaaatttatg
     6481 taagagtatt ggacaacgcc gggaactaca caacaactgt gacagataca atgaaagacg
     6541 atggaattac tgttattttg gataacaaag ttgcaggagc gcctgatgta tttgccagaa
     6601 ctgcaggcgg agagatttat gcagacgatt cctggacaaa tgaagatatc atatttacgg
     6661 taagcggcgg agagaccata tccggtgtag accactatga gtatgcagta aattatgtaa
     6721 aggataacac gaattgggat acagaaaatg cagactggaa aacagttccc agcgaagatg
     6781 ataaggcaag tatcaccgtt tctgaggata ccaatgcaac atactatttc cgtgctgttt
     6841 caaatgcaga ggtggagggt gagatcacat ccattcatgt aaaagtacag aaaacgattc
     6901 cggataatgc agaggtaaca ctgccggccg taaacggaaa gaatgactgg tatgttagta
     6961 ttccgagcat ttctgtagga gaaccgacac gtccccagta tgaagcgcag atcacaactt
     7021 attataaatt atggaacacg caggcaggag aaaaggaacc aaagtccgga atgaggtata
     7081 atcctcttag ccacccgaat attgatgacg acggcgttta tactttgaaa gtatggacag
     7141 aagatgaagc aggcaatcac tgcgaaaaag attatgtcaa ggaaataaag gtggatacta
     7201 cagcgccaaa ggaacaggaa ctttacattg tagatgcaac tgttgccggc ggtaagaaag
     7261 caatttctgt agaggacaaa aattcaattg tctatcgcca tatctatcag actgccattg
     7321 agatccgaac agcttttgat tttgatatca gtggggaaga aaagatagag tatcagaaag
     7381 tcagaagcta tgcagtgaat gacagcaagt ggtccgtata caacgaaaag acaggcttgg
     7441 ttgtaaaacc gaatgaaaaa ttcattcttg cagtaagagc aacggatcag gccggcaata
     7501 caactacaat ttattctgac ggtattattg ttgataatga agctcctgtt ggtgaaggtc
     7561 tggcgccgga gatcacgatc gcaccggatg caccgaatgc aaacggatat cataagagtg
     7621 atgttggcgt tcggatttca gtcatggatc cgccttataa cggaggtgcg tataatcaga
     7681 gcggtattta ttccggtttg gaatctgtgg tgtatcgtgt gattacagac ggcgttgtga
     7741 ctcaggaaga gactctgtat tcagcaaagg aaaatccgtc tgatgcagat ttgcagccaa
     7801 cattttcaac aaatattgtt gtcagcagca cactgaacaa cagcaataat gttattgttg
     7861 aagtggtggc agttgaccgt gccggaaacg aacgtgtgag ccgcacggca gatggtgcaa
     7921 ttcaaattga tataactgcc ccgacaatcc ggatcagtta tgataacaat agtccggatg
     7981 cagcaaatgc aaagtacttc aaagacgcaa gaacagcaac gattgaagtg acagaacgta
     8041 atttcaaccc ggatgatgta cgtctgatgc tgacaaacag tgacggaaca atcccggcaa
     8101 tttccggctg gtctatatac ggcggatccg gaaacgggga tgatacagtc catacagcta
     8161 ctttgactta tagtgcagac ggcgattata cattcgacat tgcctataca gacatggcag
     8221 ataatccttg tacagagatc aattatgttt ccggaacggc tgcgccccag gaatttacaa
     8281 ttgaccgcac agctcctgtt atcaatgtat cctatgataa taatgatgtg aaaaacggaa
     8341 agtatttttc gaagggacga gttgctacga ttgttattca ggaacataat tttgattcct
     8401 ctcgtgtaaa gatcacagcg acagcatcga aaaacggaaa gggaattaca attcctgcat
     8461 caagtgtatc ctggtccgga agtggtgata accatactgc gaccctgaat tacaaggatg
     8521 acggcgatta cacatttgat attcaattca cagatatggc tggaaatgag agcggtgctg
     8581 caaattacgg atcatcagct gccggtaagg aatttacagt tgatacaacc attgaaaagc
     8641 cgtctgtgac catcaatggc aaagacggaa atggaaaggc atttcaggat gcactcaagc
     8701 ttgcaattgg atttggggac atcaactata agtcatacga tgtaaaatta gagcgagtcc
     8761 tcaagggcaa gaaaaaagaa gatgtaacaa agaaatttat ccagaatctg aaggtaagtg
     8821 gaggttccgg agaaggtatc tttgatacct ttgaaaaaat ccaggaaaat gatggcatct
     8881 atactttgac tgtaacgctt accgacatgg cagagaatac ggaaactaca gaagtgaaat
     8941 tcacaatcaa tcgttttggc tctgtttatg agtacagcga atatctgacc aatctgattg
     9001 cagacggcgg tcaatatgtc aaggaattga cagaagatct cgtcattaca gagtacaatg
     9061 cagataaact tgtgaaggat tccctctcta ttgttgtgac aaaggatggt aaaccggttg
     9121 aaaatgcaca gtgtgatgta acgccggtaa tcaatgacaa ggtatctaca ggaaacagtg
     9181 gttggtatca gtatgattat acgatccaca aggaagatct gagtacagac ggcgtttaca
     9241 agattgtggt ttcttcggag gatgaagccg gaaacacacc tgaaaacacc aattatgaag
     9301 gtcaggcaat tctgttccgt gtggattcca ctgcaccaga gctgaccagc gtaaccggac
     9361 tggaaaagcc tattgtaaat gcacaaaatc tgacggttga ctacgatgcg tatgatacga
     9421 tcggattgaa atccattacg atctacgtag atgataagga agttgacaag atcgtagatt
     9481 ttgcaggaga tgcgaatgat tacagcggtt catttgacat tggtgaaagt aagaatgcac
     9541 agaaagttca tcttgttgta gaggataagg ctggcaatat tactgacacg gatacagatg
     9601 atttcagcag tgcttatgac tttgagcgga cggtaacggt atcaacaaat atgtttgttc
     9661 gttggtatgc aaataagccg ttgttctggg gttctgtagg cggtgttggc gtactgggaa
     9721 tcggagcagg tctattggta ttcctgaaaa agaagaagaa aacagcataa tattttcccg
     9781 ataaaatgat ggtagcgaac ctgaaatgtg attattttgg gtttgctatc catcacaaat
     9841 gacagttgct gaatatctgt caactgttat ttgtgatgga tagcaaaaaa tgcaaaggaa
     9901 gtgagaaaga tgaatccatt gattgaatgc ggcgtaatta ctgagaaaca atccggcgta
     9961 aactttatgt atgtgctgaa tgacaataaa gactttctca ttacagagta taaggtactt
    10021 cagaatcaga gtgacagtaa cttcattccg tgcatgaaaa tattatataa t
//
LOCUS       DAWENO010000073         9528 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_2989_length_9528_cov_12.053521, whole genome shotgun sequence.
ACCESSION   DAWENO010000073 DAWENO010000000
VERSION     DAWENO010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2989_length_9528_cov_12.053521"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2417 a         2730 c         2283 g         2098 t
ORIGIN      
        1 tgtcgatctc ccgatccgct cttccacttt ctttcattca tttgtcctct tgactcccgc
       61 tcccgtgcga cagctttatt attataccac gctcgctcga ctttgtcaag cataagttta
      121 tgaacattaa aatctattta ttgtacattt tatacatagc actactttaa ttaccccaaa
      181 caacgcaaaa agcccgactg tttccaatcg ggctttcgca taattctatc taatcttctg
      241 ttatgcactc tgactgctgc tgtccatatc gatcttgaac gcatcataac gcttgattgc
      301 gctgtcgttc ttctcgacct tctggtcagc tgtccagccg tccagcatat cctcaaacgc
      361 atcgctgtat tcctgcgaaa tgacagtatt ctgctgtgac tctgtccaca gatcgtcttc
      421 cgtcatacgg tccttgatat cacggctcat tacgatatag tacgcgtcat catcttcaat
      481 gatcgttgca tcgccatagt tcttctgtcc atcgccgaaa gcatagtcgt atacattctt
      541 gcatggtgta taggtgatat cctcttcctt ggtatcttcc tttgtggtca cctttgcaat
      601 gatctgctcg ttggcatagg gtgcagttgt tgtggtagtt acctcagaac cattctcgtc
      661 agtctccaca gctgcctctg tggttgtctc ttccgcagca gtctcttcgg ttgctgtggt
      721 ctcctcagaa tcgctgtcgc cggcagtttc cgtcgctgct gcgctgtcct ctgcggcagc
      781 ggtagttgta gttgtggttt cttctgtggt cgtagttgtc tcttctgttg ttgcaggaat
      841 ctcgttgccg tctgcatcgg ttgccgatgt tgcagttgca gcagcagcct cttcggaaat
      901 cgaagtcacg taagcattgt agtcgctctg aacagtgtcc atctcgtctt ccatggcatc
      961 ttcatcgccc ttgagtgcgt tgatctcgcc cagatagtcc tcgacctttg ccttcatatc
     1021 cttcttgccg gcatcatcca gcgcctcgcc gttgccgtct gtcagattga acttgatgta
     1081 ccgtacacgg gcattgtttt cctcatagta ctgtttcaga tcatcctcgg tggttccctc
     1141 ttcgccgtcc agaccgtagt aatacaggaa cacatcattt gtcatatagg tgttttccag
     1201 aacgctctgt actgagcttt tcgcaatacc gttcttttcg tacagttctc cgtttgtatc
     1261 ccaaaacgaa tcaatggtgc tggagatctc cttgttctct tcttctgtca gtgacagatc
     1321 cagctcctcg aacttctttt cgattgccac atagcgctgg cagtattcca gtgccttgtt
     1381 cttgatccac tcttccgagc tgacgccgtc catcttctgt tccttgacaa catccttgtc
     1441 cgatacatcc agttccgaat tctcagactg gagcgtctgt gtcagttcca tataggatgc
     1501 gtaagaatag taaatgtaaa tgccggcatt cacctgtacg ccgtcaattg tcatggcata
     1561 agccgtaccg cttccggcag tgcatcccac gcctgcggca gtcattgcag cagcagccag
     1621 aacagccgca gcttttttcc atctgaacat agttatttga tacctccgaa taaaataaac
     1681 ggtcttgcag agaaaccgga ccgcccgaat gctcccggga caagccgctc cgcaatccaa
     1741 tactttttta ttatactata tttttttgca taagtccata ctttcacgaa atttttttca
     1801 gattctcaga aaactttgat tttcgattga tttcggcgtg catttgtggt atagtaaaat
     1861 cagaaactga gagaaaggaa ctttgatatg aaagcattga ttaccggtgc gacttccggt
     1921 atcggaaaag ccattgcaca ggaactgagc cgccgtggcg tctcgctgat ccttacgggg
     1981 cgcaatgaaa acgccctgct ccagatgcag gagaccctgc ccgttcacac agagatcatc
     2041 gccctggatc tggcagagaa ccaggcgccg ttcaccctgt acgaattctg caaaaacaag
     2101 cacgtggaca tcctggtcaa caatgccggg ttcggcgttt tcggcgaatt caccgagact
     2161 gcgctccagg aggaactgga actgctgcag gtcaacgtcc gtgcgctcca cattctcacc
     2221 aagctgtttc tgcgtgactt caaaaagcgc aacttcggac gcattctgaa cgtggcgtcc
     2281 agcgccggat tcctcaccgg accgctgttc tcctcctatt acgcttcgaa aaactatgta
     2341 gtccggctct cactggcgat tgcggaagaa ctccgccggg aaaagagccg tgtcaccatc
     2401 agcatcctct gccccggacc agtggacacc gggttcaacg accgtgccgg cgtacagttc
     2461 cgggtaaagg cgctgacacc ggaggcagtt gccaaagagg cagtgttcgg actgctgacg
     2521 ggaaaactgc tcatcattcc gggcatgatg atgcgtgtgg gcgttgccgc atccaagctg
     2581 gtgccggaaa agctgctctc ccgtatcctg tatcacgtcc aggcagccaa acaaacaggg
     2641 aaaaaataag aattcggttt cttgaaaagc acagcggacg gttgaaagcg cagtctgctg
     2701 tgctttttct tttgcataaa aatgttgaca aaaaagcaat gcaatgttat aatatatttg
     2761 ttcgcattca caatgcaaat ttggaaagaa ggaatgaaat cagatgaaac tcaaaaaact
     2821 cctggcggca gtcaccaccg gtgtgcttgc tgtcaccagt ctgtcccttt cgggactggt
     2881 cagctccgca gaggtgcagg aactgatgaa cgacacgttc gaaagcggct acggcgcatg
     2941 gaaaggcgta ggctcttccc tgtccctgtc cacagagcag tcccacagcg gcggcacatc
     3001 cctgtactgc tatgaccgta ccgccagctg gggcgcaccc cgctgctcgc tgaccggaat
     3061 cgtggctgcc ggacagagct acgagatctc tgccagcgcc atgtacgagg gcagcggtca
     3121 gcagaacatg gcgatcaaga tgatctacac cgacgccaac ggcacagacc actacgacca
     3181 ggttgccgct gcacaggcaa ctgccggaca gtgggtggag atgaagggca actacaccgt
     3241 tcccaacggt gctaccggta tgatcctcta tgtggagatg tccaatgcag gcaccgacca
     3301 gacctattat attgatgatg tggtcatcaa gggcgaaaag acagagattc agctggacga
     3361 caagttcgaa tccgacttcg acaacaactc cacccagaag tggaacggcc gtggcagcgc
     3421 caaggtagaa ctttccacca agtacgccca cagcggcacg accagcctgt atgtctccgg
     3481 cagaacccag ctgtggaacg gcgcaacacg cagtgcgtcc gacatcatgg aggcaggcgg
     3541 ctactacaaa gtcggcacat acgttctcta tgacggcgac cagtacaccg acacccagaa
     3601 attctccatc aacttacagt acgacctcaa cggcaaggaa aactactaca ccattgccac
     3661 cgagactgca aacaagggcg aatggaaata cgtgggcagc gaattcaccg taccggaggg
     3721 tgcgaccaac ttctatgtct atgtgcagac cggctacacc agcgctccca aggaacagga
     3781 tctcatgaac ttctacatgg acgatgcagt gggcgaacac ctgcccgacc cggcaatcca
     3841 ggacgatatc gcatctctga aggaggccta ttccgactac ttcaagatcg gctgtgcctg
     3901 cgccggctca gagttcgcac agggcgcaac caaggacctc atcaaaaaac actacaacag
     3961 tctgaccctg ggcaatgagc tgaaaccgga cagcgtgctg gatctggcgc tgtctcagaa
     4021 gtacgtggca gagaccggcg acgacaccat gccccagatt tccctcaacg aggcagacga
     4081 gatgctgaaa tttgccggcg aaaacaagat tccggtacgt ggccatgtgc tggtatggca
     4141 cagccagaca ccagactggt tcttcaagga aaacttcgac cccaacggtg catgggtctc
     4201 caaggacaag atgaccaagc gtctggaaaa ctacatcaag accgtcatgg aaacgctggc
     4261 aaaggattat ccggatgtgg aattctacgc atgggacgtg gtgaacgagg ctgcctccga
     4321 tgccggaacc attcgtgatg ccggctccaa caacgaggtc aacggacagt ctgcatgggt
     4381 caaggtctac ggcgaccagt cctatatccc gctggcgttc gaatttgcca agaagtacgc
     4441 accggcaggc tgcaagctgt tctacaacga ctacaacgaa tactccccca acaagcaggc
     4501 gtacatcatc agtgacattc tgaaaccgct ggtggaaaag aatctgatcg acggcgtggg
     4561 tatgcagtct cacatctcca tgagttatcc caccattgat ctgtacaagt ccgcaatgca
     4621 gcagtatgca gacctgggac tggaagtaca ggtcaccgag ctggacatct ccgagaagtc
     4681 caacgaatac gccgaccagc tggcactggc acagcggtac cagggcgtat tcaagatgta
     4741 caaggaaatg aaggattccg gcgtgaacct gtccgctgtt gttatctggg gcatcaccga
     4801 ctccaccagc tggatcggcg gctatcccct gctctttgac aaagattacc aggcaaagcc
     4861 gtcctattac gccgtgatcg acaccgactc cgaggtagaa aagctccaga ccatgacggc
     4921 ataccgctat gacggcacag atgccgatct ggaacgggca ttggaaatcg gtacggcaca
     4981 gtacctcagc accaaaaccg gcagtaccag cacttatttc aaggcagcct ggtctgacga
     5041 aaacatggtc gttcgtgttt acaatccggc tgtttcagca gatgccaaaa actcctatgt
     5101 tgaaattttc ggaaaccaac tggattctat gggaggtttc ccgattgacg accaaactat
     5161 tacttccgct atggaatacg tagacctgaa atgggaatcc aacagtaatg attggaaccc
     5221 gaaaagcggc agcactgttc acttagatgt attcaacaat aatgctgcat ggaattgcat
     5281 tgatgtagca gattacgttt ttaacaatag tgacgtacca tctggtgcat tgcccacctg
     5341 cggtattgtc accctggcag aacagcccaa gtatgccgag gcaaccaaga ccgaaacacc
     5401 cattaccatt gacggggaca tcgataccgc atgggcagat gccaacacca ttgatgtcaa
     5461 cacctactcc atgggcaacg gtgcgaccgc tgtttccaaa atgctctggg atgagaacta
     5521 cctctatgtg ctgaccgaag tcaccgaccc cgtactgagc gtggcaagtg ccaacgccta
     5581 cgagcaggac accgtggaag tattcttcga cgagaacaac cacaagactt cctcttatga
     5641 gagcgacgac attcagtgcc gcatcaacta cgagaacgac aagaccgtta ccgacggacg
     5701 ctccactgac gcattcctct ccggcacgaa aaagaccgac aagggctaca tcgtcgaagt
     5761 ggcgattccg tacaccattg gttcgttcca cgccaaccag atcgtaggct ttgatgtaca
     5821 ggtcaacgac gacggcaccg gcgacggcaa gcgtaccagc attgccaact ggaacgacct
     5881 caccggacag ggctacatca acaccagcgg tttcggcgtg ctgaaactgg tgggtgacgg
     5941 cacagttacc acagacacca ctgtgacaac aactaccacc ggaacaacct ccactacaac
     6001 ggaaaccgtc acctctgcca ccacctccgg cagcagcaca ggcacttcag aaagcggcga
     6061 gagcggactg accctctacg gcgatgtgaa cctggacagc cgtgtagaca tcaccgatgc
     6121 cgttctgctg aacaaggcag tagccggtgc agtggaactg gaatcccagc aacagaagaa
     6181 cgcagactgt gacgccagcg gcgacctcag cagcaacgat tctgtgatac tgatgaagtt
     6241 cctggtatct cttgtgaaaa cactgcccag cgctgaataa gcgtacactg acaatctaat
     6301 atgatgtaac gactaaaagc ctcccttgaa aagggagggg aaccagcgaa gctggtggag
     6361 ggattctgat gaaatttttt cttgaattgc agatttcaca gcaaaatccc tcagtcagcc
     6421 tgcggctgcc agctcccttt tcaagggagc ctaacctaat ctataaaatt ataaaaatta
     6481 caatatacta gagaaaatta gaaaagcgga gattccatac accggaatct ccgctttttg
     6541 taatatgtga tatatgagct tatttatccg attccttatt atagaaaaac atgaccactg
     6601 ccatggcaat gatgatgcaa taccccagaa tactcagcca gtccggaatc tgcgcaaaca
     6661 gcaggaatcc cagtccggcg gcaaaaatga tctgggtgta gtcgtaaaca gagatttcct
     6721 ttgccggagc atagcagtac gccgaggtca cgccgaactg tccgccggct gcggaaacgc
     6781 ctgccataac cagacacagc agctgtttcc acgtcatagg ctcaaaggca aacagcagat
     6841 acggcagcgt caccacgcag gaaaacgccg agaagaaaaa gacgatgaac gccttattct
     6901 ccccgatctg tcccagacgg cgcaccatgg tgtatgccag tccggcaccg aatccgccca
     6961 gcagccccac aagagacggg aacaaatgcg cattggcaaa gctgggcttg atgatgcaga
     7021 gcgccccgac aaatgcgccc aggacgatca gaccctgttt ccagttcagc ttttcccgga
     7081 ggaagagcca tgagcagagg atggcaaaaa agggcgacat cttattgaga atggacgcat
     7141 ccgaaagcgc cagatggtcg atggcgtaaa agttgcacag gatgcccagc gtcccggcag
     7201 atgcacggat cagcagatac ggcacagcgc ccttttttgg acaaaagctg ccgtgttccc
     7261 gcagcagcat caccaacgcc agcaagagcg ccacaaagtt ccggaaaaag cttttctcaa
     7321 taaacggcag gtcacccgac agccgcacaa aggcattcat ccacgcaaag caaaacgcag
     7381 acaaaataat actgatcatg cccttctgcc gatcggatag ggaaattttt gacatgagat
     7441 atcacgactt ctttcattga cgagttttat ggttcacata gccgccggta aatttggacg
     7501 ccaggaacgt ctgctctccg tataagccat aatagccgga cagcagatag ctgacggcga
     7561 tcgccagcag ataatacggt gcgcctgcca taccgaacag ctccgctgcg atcatcatag
     7621 aggcaagcgg acagtttgtc acgccgcaga acagcgccgc catacctacc gccgcacaga
     7681 gtgacggcga aatgcccagc agatgcccga agagacagcc gaaggttgcg ccgataaaga
     7741 acgacggcac gatctcgccg cctttgaaac cgccgcccag ggtcactgcg gtgaagatca
     7801 tcttcgccag gaacgcatac cagaccacct ctccggcaac tgcccggcgc atcacttcac
     7861 cgccgatgcc cagatagtcc tggttccgca ccaaaagcgc cagcacccag atcacaccgc
     7921 ttgcaatcag aatccgcaca tagggatttt tgcagcgctt tttcagcagg tgttctgtct
     7981 gatggagcag gatgcagaat aaaatgctca ccgccgcaca gcatacgccc agcagcaaaa
     8041 ttttcagcgc cggaacgacc cggaatgccg gaagatcacc cagggcatat tttgccagcg
     8101 gaatgccgca gcggtctgcc agccaggacg ccgtcagcga ggcaatggta cacggcacca
     8161 gcgccgcata gcgcatggca ccaacgcaaa ccacttccat ggcgaacacc ggcgcagcaa
     8221 tgggcgtgcg gaaaatggca gaaaatgccg ccgacatacc gcagagcatc agcacctgtt
     8281 tatcccgttc tgccagccgg aacagtctgc caatcacgct gcccacgctg ccgcccagct
     8341 gcaatgcagc gccctctctg ccggcgctgc cgccgcaaaa gtgcgtcacc acggtggaaa
     8401 cgaaaatcag cggtgccatc tgtacaggca attccgtctc cgcacgcaga gacgccagca
     8461 ccatgttggt tcccttgtca ttctggtcat ggctgatgcg atagagccag acgatcagca
     8521 gaccgcccag cggcagcaaa ccgatcaccc accaatgccc caggcggaac cccgttgccc
     8581 actggatggc atacacaaaa gcgatcccga taaagcccac cagaatcccc atcagcatcg
     8641 aataaaacac ccagcgcacc aacagccgca gatgcgacaa aatgtgaata ccgctatgta
     8701 tcagccgtcc cttccagcgg gagaaaaaat aatccccgga acgcataccc gaacattcct
     8761 cctttgtgca aaacacaatc ccctgaaaag cagtgccttc cagaggatca ttttgtttcg
     8821 tttcctgtca atcaataatt ttccgctttt acctggaaat aggactgcgg atgctggcat
     8881 accggacaga cctccggcgc tttcttgccg atcacgatat gaccgcagtt tgcacattcc
     8941 cagatcacgt cgccgtcctt ggaaaaaaca cactcgtcct caatgttggc aagcagcttg
     9001 cggtagcgtg cctcgtgttc cttttcgatc gcacccactg cctcaaacag tgcggcgatc
     9061 tgtgtaaagc cctcagcctt tgcctgctcg gcgaactccg catacatatc ggtccactca
     9121 tagttctcac cggcagcggc atctttcaga ttctccgttg tctccggcat ctgaccaccg
     9181 tgcagcagct tgaaccagat ctttgcgtgt tccttttcgt tgtttgccgt ttcctcaaac
     9241 agcttgccga tctgtacata gccatctttc ttcgccttcg aagcatagta gttgtacttg
     9301 gtgaacgcct gcgactcgcc ggcaaaagca gccagcaggt tcttttccgt ctgtgttccc
     9361 tttaattctg ccatatctgt ttcctccttt tattggctgg tgaactgcga acgctgttcc
     9421 tctgcctttc ggcaggcagg acacagcccc gtaaaaacaa tgtgatggga ctccaccaga
     9481 aacgcctcgc cgcccacgat cttactgcac aagtccgtct gatacggc
//
LOCUS       DAWENO010000074         9446 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3026_length_9446_cov_9.998829, whole genome shotgun sequence.
ACCESSION   DAWENO010000074 DAWENO010000000
VERSION     DAWENO010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_9446_cov_9.998829"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2756..2765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4542..4551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2587 a         2005 c         2436 g         2398 t
ORIGIN      
        1 tacagtgcac acctttccgg aaagccatcc agataatgaa gtcaccactt ttcgggttga
       61 tattgatgtt tcaacctgcg gcgagatctc ccctttgaat accttggatt atctgatccg
      121 cagctttgat tcagatatta ttaccattga ctatcgggta cgtggattta cgagagatgt
      181 ttccggtaaa aagtgtttta tggatcagga aatgacgtcg atccaggatt ttatcagtga
      241 agaaattctt cttcgctatg atgcgcagga tgtcaatgtg tatcagtcaa accttttcca
      301 cactcgtatg ctgattaagg aactggaact gcaaaactat cttttcaaga ccgacgctta
      361 tgaactcgat ccacgggtgc gattggatat tacaaatcgt ctgcgccggg aaatgattga
      421 aatattcagc ggcagaaata tctattgagg aggcgtgcca ttatgaattg gacgcagcag
      481 gatgcgcctt taatggatgc gttgaaagag catctcatga atcgtgtggt tcggtttgat
      541 gtgccgggac ataaaggcgg tcgtggcaac catgagctgg ctgacttttt gggcgaaaga
      601 tgtgtgcaga ttgatgtgaa ctccatgaaa aatctggaca atttgtgcca cccggtttct
      661 gtacttcggg atgcacagca gcttgcagcg gaggcgtttg gtgctgcaaa tgcttttttc
      721 attatcaacg gcgcaaccgg tgcatcccag gcaatgatta tgtctgtctg caaagctggc
      781 gaaaaattga ttatgccccg aaatgtacac cgcagtgcca tcaatgcgtt agttgtaaat
      841 ggtgcggttc cggtctatgt ggatccgggc gaaaataagg atctgggtat tccgctggga
      901 atgtctgtag aggcagtaga acgtgcaatt ctggcaaatc cggatgcaaa ggcggtattg
      961 gtaaataatc cgacttacta tggtgtctgt ccggatattc gccgtattgc caaacttgcc
     1021 catgaacacg gcatgctgct gctggcagac gaagcacacg gcacacattt ttactttggc
     1081 gaaaatctcc cctgctctgc tatggctgcc ggggcagata tggcatctgt cagcattcat
     1141 aaaaccggcg gcagtctaac acagagtgct atgctgctct gtgcggaggg catcaacgca
     1201 gactatgtgc ggcaggtcat caatctgacg caaacaacga gcgcctctta cctgctcatg
     1261 gtttccctgg atattgcaag gcgaaatctg gcgctgaacg gcagagaaat gtaccggaaa
     1321 gcggtgcagt atgtagatta tgcacgggaa gaaatcaacc gcatcggcgg gtattatgtc
     1381 tacggggacg aactgtgcaa cggcgattcg ttttatgcgt tcgaccggac aaaattgtca
     1441 attcataccc gaaacacggg tctggcaggc atcgaagtgt atgacctgct ccgggatgaa
     1501 tacggcattc agatcgaatt cggcgacatc ggcaatatcc ttgccattgt cactgccgga
     1561 gatcgggcgt tggagatcga acggctgatc tcggcattgt cggagatcaa gcgactgtac
     1621 ggcggtgacc cgactggtat gctgcaaagc gaatacctgg cgccggacgt ggcacttccc
     1681 ccacagcagg cgttttatgc ggacaaaaag gctgtgccac tggaacagag cgtaggcgaa
     1741 atttgcagcg agtttgtaat gtgctacccg ccgggaatcc cgattcttgc tcccggcgaa
     1801 cgaatcactt cggatattgt gaattatatc cagtacgcca aggaaaaggg ctgttctctg
     1861 accgggccgc aggatatgga gattgcgaga ttgaatgtgg tgaagaagta gggcatctgt
     1921 ttgtttggta aatcggagtt tagtggtgaa ctgcatgatt gaagaaatag agaagtcatt
     1981 tgtgaaaaga tttataaaaa agacaaaaca ggacagactg ctttttgagt tgagcggaaa
     2041 aaaacggcag cacggaatag agcgtttttg tcacaacaca gaagatttga tagatacgga
     2101 aagaatcgct tatagcggaa ataatttgtt tctcgatgaa attctaagaa tcataaaaca
     2161 gtataaagtg ccggaatcat gctatattat tgcttgccaa aaagagctag acaaaaaaca
     2221 cgtttcattt gaggatgcat taggtctcgt attaggaaat gggatggcag ctattataat
     2281 atgcaatgac tttgctgtca ttgaaacaga acagtactcc ggcacaccat ttcgctatat
     2341 tttacacaga taactttcag ttaatcaagc gtgatacatc catgaaaaga gatatacata
     2401 tgaaacattt ttggggcgat tatcaaaacg aatcatatcg tgtaggctgt actggtggaa
     2461 caatctatgt ttatgacaaa gccggaaacg aaattgcgag attcaaagat tttccctatg
     2521 cgtatacagc cgcttttatg ccggggagaa atattatcgc agtgaaatct acagaaggaa
     2581 tcttaggatt ttatgactta gatacgcttt ctttacgcaa aaagattact gttacgaaaa
     2641 tcggcggaca agatgaaggc tttggctttt cgcctgacgg caggttcttt tacaatatcg
     2701 aaagtccgac ttgcttcgtc agaacacaac ttagtgtata tgaaacaaga gaattnnnnn
     2761 nnnnncaata cgtgctattt gctggggtat atgcgtgatg atgcggatat gtttgactac
     2821 ggtttcattg cagtatttga tgctgaaagc aaagcagtaa aaaacattcg tgccattgac
     2881 agaaaacaac atgactattt atcggattac aaacactggg agttaagcgg atttactgaa
     2941 aagtcacgca agtggagttc actcaaattc ctggagcata ttgacaggac aagcataaag
     3001 gaagtttaca aatcaaagga ggcataaaaa tgtttcgacc gatgagaaga tggaaacagc
     3061 agctttctga cgaggactgc attgcaattc tgaaaaacga gccgagaggc atcttatctg
     3121 tactgggcga tgacgggtat ccttacggca ttcccatgaa ccactggtac tgcgaggcag
     3181 acggcaagct gtatttccac ggagcgaaaa caggtcacaa gatcgatgcc atttcccaat
     3241 gcgacaaggt ttcctactgt gtccatgacg agggctatcg ggaggacgga cagtggccgc
     3301 tgcacattaa aagtgttgtg gtattcggtc gtatgcagcc agtgacagat gcagaaaagg
     3361 aactggagat ctgtacgcat ctatgccaga agtttacaga cgacaaggcg tatctggaac
     3421 atgaattgca gcattcccgt ccgaaggtgc tgtgccttgc actgacatcg gagcatatga
     3481 caggaaaact ggtcaaagaa tcctaatcca aaaggcaaag tgaaaggagt tttgatatgg
     3541 acatctggta taccgaaaat cacacggata acgtccggct ctccattcgc attgacaagc
     3601 agcttgccag tgtgcagagc gaattccaac gaattgacgt gtttgattcc gtggagttcg
     3661 gacgcattat gacgctggac ggtctgctga tggtaaccga aaaagacgag tacatttacc
     3721 acgaaatgat tacccatatt cccatggcga ccaacccgag catcaaaaag gttctggtga
     3781 tcggtgccgg cgacggtggt acggtgcggg aactctgccg ctacccttct atcgagcaga
     3841 tcgatatggt ggaaatcgac gaactggtgg tgcatctggc aaaggagtat ctgccgttta
     3901 cagcatgcag tctggacgac cagcgggtac atatttacta tcaggacggc ctgcggtttg
     3961 tccgcaaggt ggaaaatgaa tacgacctga ttattgtgga ttctaccgat ccgttcggcg
     4021 tgggtgaagg tctgtttacc aaggaattct atggcaactg ctataaggcg ttgaccgagg
     4081 acggcatact ggtcaaccag catgaaagca cattctacac gtcctattcc aacgccatga
     4141 aacgggcaca cagccgcatt aaaagcttct tccctatcgc actggtatat caggctcata
     4201 tccccactta tccctccgga cattggctct ttggatttgc ttcgaaaaag tatgaccctg
     4261 tacgggattt gcaggcggac tggtggaatg ggctgggtat caaaaccaga tattacaata
     4321 cgattctgca tcagggctgc tttgccattc cgaacaacgt tcgagatgct ctgcgggaat
     4381 ctgagcgtga cagaaaataa ggagcaaaga tgggactatt ggacatattt aagcgaagag
     4441 acagtgtacc ggagaaagcc accacatgtg aaaaaacgga ttctggtgcg aatatggtga
     4501 aagaaatccg tgaggagatt ctgcaaaaga cggcaatccc cnnnnnnnnn nagattgcag
     4561 ctgacagata ccaaaccaag tctgtttgac agtaaggttg gcggtattgg atatgttccc
     4621 cacgatgcga aaattccgga ggacagtgag ggcacacaac tgcggctgct ggcacagatc
     4681 gactgtgcag agatcacgct ggaagatttc ccccatcagg gactgctgca attctggatt
     4741 ctgaacgacg atgtcagcgg actgaatttc gatgacaaca ccttgcagga cacatttcgt
     4801 gtgctatatt tcaaagagcc ggatcagacg gtcacagaga aagaagtctg cgaaaaaatg
     4861 gcggcatctt ctgacgaaga ggatctcttt ccggtgagag gctgttatgg actggcattc
     4921 tcggaagatg tggacacaat ttcttacagt gattatcggt ttgaaagctg tattcgaaaa
     4981 ctggtacaac aggattatcc agaggaggct gaaaacctgc tagaaaatat ggatgacttt
     5041 ttcgacgggg gcagcggtca taagatcggc ggttaccccg gctttgcaca atgggacccc
     5101 agaagcagtg aagacacgca tgatgtattg ctgtttcagt tggacagtga catgggaacc
     5161 gactggaaaa ttttatgggg tgattgcggc atcggcaact tcttcatcaa ttgggaaaag
     5221 ttaaaaaact gcgatttcag tgatgtgctt tatacctggg attgctgcta atcaatattg
     5281 ttatttttga atttttggag gtatatgaac atgggaaaag cgttaattat cggagccggc
     5341 ggcgtggcag gcgttgtcat tcacaagtgc tgccagaaca gtgaagtgtt tacagagatc
     5401 tgcattgcca gccgtaccaa gtccaagtgt gacaagttca aggaagaatt gcaggacaag
     5461 acggctacca agatcaccac ggcacaggtg gatgccaatg atgtagacca gctgatcgct
     5521 ctgatcgagc aggagaagcc ggacattgtc atcaatgtgg cacttcccta tcaggatctg
     5581 accattatgg acgcttgcct tgcaaccaag acggactatg tggacaccgc aaactatgaa
     5641 ccggaagata cggcaaagtt tgagtacaag tggcagtggg cttatcggga acgcttcgaa
     5701 aaggctggca tcacagctct gctgggcagc ggctttgacc ccggcgtaac tggcgtattt
     5761 tgtgcttatg cccagaagca ctactttgac gaaatcaatt atatcgacat tctggactgc
     5821 aacggcggtg accacggcta tcctttcgct accaacttca atccggaaat caatattcgg
     5881 gaagtttctg caaagggcag ctacatcgaa aacggcgaat gggtggaaac cgagcctatg
     5941 gaaatcaaac gggaatacaa cttcgatcag gttggcgaaa aggatatgta cctgctgcac
     6001 cacgaagaac tggaatctct ggcactgaat atcaagggca tcaagagaat ccgcttcttt
     6061 atgacatttg gacagagcta tctgacacac ctcaagtgtc tggaaaatgt aggcatgact
     6121 tctattgagc cgatcatgtt cgagggtaag gaaatcgttc ccctgcagtt cctcaaggca
     6181 gttctgccgg atccggcttc cctcggtccc cgtactgtgg gcaagaccaa catcggctgc
     6241 atttgtcagg gtgtcaagga cggtaagccg gtgaactact acgtctacaa tgtctgcgat
     6301 catcaggaat gctacaagga agttggctct caagctgtct cctacaccac aggtgttccg
     6361 gcaatgatcg gtgcaatgct ggtaatgacc ggcaagtgga aaaaaccggg cgtttataat
     6421 gtagaagaat tcgatccgga tccgttcatg gacgcactga acaagtgggg gctgccgtgg
     6481 actgagaatt ttgatccggt actggtggat taaggcgatg caacaatgta ccatgtaaaa
     6541 ctacttctga aatacactgt aaaggaacag attttctacg aagaatccat tttgcaggtt
     6601 gatgccgact cttttgatgc ggcttatcaa aaggcagaga actacgtcag ccggatgatc
     6661 gaaccccaac gcaaaaatat gtatggcgac acagtacatt gctctatcgt ggattatctg
     6721 gattgctttc aatctgataa tatagaggat gatgtacagg aagtgtattc cagaatcttt
     6781 cagaatcatt cggaattttc cgaaacgaag ttggtagaac ttcttagcga ttcctgtaca
     6841 cgggaagaaa tgctgcccct gcggtatttc cccgatccaa aataaaggca ataccacata
     6901 aagcctgatt taagacgtgc atttcatttt tatgtgaaca cgctctgata tgaatccctc
     6961 aaattaagag aagcgttccg catcaagcag aacgcttctt tttcaagaaa ggaattttga
     7021 aaatggactt tgaatcttta tttgacattc ccattgatga tctgcatacg ccttgctatc
     7081 tcactgatgc acggctgctg caaaagaaca ccgcattgct gaaacaaatc cagcagcgaa
     7141 ccggctgcaa aatcctgctg gcacaaaagg cgttctcgat gttctccgcc tacccgctga
     7201 ttcgggacga tctcgccgga acgactgcca gcggattatt cgaggcaaaa ctgggacgag
     7261 agcacttcgg gaaagaagta catgtgttct cccctgccta cacgcaggaa gatttcacac
     7321 agctgctgga actctgcgac catattgtat tcaactcgtt tggacagtgg aaaaaataca
     7381 ggcagcaggt gcaagatgca acccggaata tttcctgcgg cattcgcatc aatcccggct
     7441 atgcagaagt agaaactgat ctatacaacc cctgcatttc cggttcccgc atgggtgtac
     7501 cgctggaaca aatggaagag gaaggttttg acgggctgga cggcattcat tttcacacga
     7561 tgtgcgaaca gaattccgat gtgctggagc gtacactgga tcagatgctg ccgaaatttg
     7621 ataagtggct gaaacgctgc aagtgggtaa actttggcgg cggtcatcat attactcgtc
     7681 cggattacga tatagagcgt ctggtgcggt gcattgagcg tgtacgggat gcctatggcg
     7741 tacaggtata cctggagcct ggagaggctg tggcgctgaa tgcaggctat ctggtggcaa
     7801 cggttcttga tattgtgcat aatggtatga acattgcaat tctggatgcc tctgctgcct
     7861 gccatatgcc ggatgtattg gaaatgccgt atcgtccgca tattctttac agcggcaagc
     7921 cggaagaaaa ggtgcatacc tatcgtctgg caggaaacac ttgccttgcc ggggatgtga
     7981 tcggcgatta ttcatttgac catcgcttac agccgggtga caagctggtg ttctgtgata
     8041 tggcgatata ctctatggta aaaaataata cgttcaacgg cgtccccctg ccggatattg
     8101 cgctctatca gaacggaaaa attgaattgg tgaaacattt tggatatgcg gattttgagg
     8161 gacgcttatc atgagttttg atgcggcaca ctgcacactt tgtccgagac agtgcagtgc
     8221 agacagaacc aacggtgtgg gtgcctgtca ttgcggaacg gaaattcgca ttgcaagagc
     8281 ggcgctgcat tactgggaag aaccgtgtat ttccggcaaa gatggctcgg gagcggtctt
     8341 ttttgccgga tgtgctttgg gttgtgtttt ctgccaaaac aaagaaataa gcaggcgtgc
     8401 agtggggaaa gtgtttacag ttggtcagct tgcagatgcc tttttgagat tacaggaaca
     8461 gaatgcgaac aacatcaatc tggtcactgg cagtcacttt acgccttgga ttgtacaggc
     8521 tttagagctt gcaaagccaa agctacacat cccggttgtg tggaattgcg gcggttatga
     8581 atcgcttgag attttgcata tgctggatgg tttggttgac atctatcttc cggatctgaa
     8641 attttataca gctgaaacgg cgaaagctta tgccaactgc ccagattatt tttctgttgc
     8701 ctcgaaagga attttagaga tgtttcgtca ggtcggagcg ttatcgtgga acggcgattt
     8761 gctggaacgt ggactgatgg tgcggcatct ggttctgccg gggcatcgaa aagagtctat
     8821 gcaaatttta gattggctgg cagatgcact cccgaaagcg gactttttgc tgagcttaat
     8881 gggacaatat acaccgcccg atgaaccgtt gtcagacaag catttgaatc gccgtattgc
     8941 gacatttgag tatgagtctg tgcgaaaata tgcggtacag ctggggcttg acgggtacgg
     9001 gcaggaacgt tcttcggcat catcgttgta cacgccggag ttttggacgg agtaaaacga
     9061 aaacggatgt gaaatcttaa atcaatttca catccgtttt cgttttgatg gggtctgctt
     9121 tctgatgttc atgcagacgc ttaggcaata ttacatattt ttactatcag tttttattcc
     9181 ggctgaattg catcgtagcc cgtttctttg gtacgaatgc gcatcgcctg ccgcacatcg
     9241 tagctaaaga ttttgccgtc gccaatatga ccggtaaatg ccgctttttg aattgcgtca
     9301 atggttttct tttcccactc ttcagaagaa accagaactt cgatcttgat tttcgggagc
     9361 atttgaatct ctacctgctg gtgtccacga acgtaggtgt attccttgaa accacgctgg
     9421 gtgccgcagc ccataacctg ataaca
//
LOCUS       DAWENO010000075         9094 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3181_length_9094_cov_9.577719, whole genome shotgun sequence.
ACCESSION   DAWENO010000075 DAWENO010000000
VERSION     DAWENO010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_9094_cov_9.577719"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3529..3538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8151..8160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8736..8745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2236 a         2428 c         2234 g         2166 t
ORIGIN      
        1 aattgacaaa tcaaatttgg tagtatatcc cggaaaagca atgggattga ctatattcga
       61 gcgtgttccc gtaaaaagtt tttgatgaaa atgtgcatta cattttttat ggaaacacac
      121 tccaaaagct gctgcacctc gcaatgcagc aaaatcaaaa atttttagga ggtcattatg
      181 cgagagattc actggaaaaa gctgatcggc gcagcatctg ccgctgtgct tgcagctaca
      241 caagtcgctc cgaccatttc ggcatttgcg gaagaggcaa gcacaggcga ttatgtctac
      301 tgctatgcaa ccatgacatg ggcggaatac tggaagaatg agggcgttta tctggacagt
      361 gccgcaaacg gcaactggga ggcatcctcc gcagagagcg acgcacacaa cgaaacagat
      421 atgggtgcgt ttgatgcggt tacacgtgca accagcaacc acggtctgca cagaggcagc
      481 tatcagtgcg ctgttaaggt attcgatacc gacggcaaca gctacgacta caccggcaag
      541 ggtcagggtg aatccgttga tgttgtcaag aataaggaga ccggcacagc agacattacg
      601 gttgatggaa cagttgttgg tacatacgac cattctgtta tcagtggtcc gaagtatgtt
      661 ccggttgctg ttccggcagc ggattacgag gacttcaaga aagcttatac cgtttacgaa
      721 aacggcacag aactcaccgg cggctactct gagggcaatc ttgtcaacat tgatgagact
      781 gtgaaggttg acgccaacac atccggcatc aagaccgcag aaaagcagga ggacggcaca
      841 ttccagttct ccaagcgtga aacagctgca actgctgacg gcaaggtggt agacaccagt
      901 gcggttgaga tcggcgaagt acagcacgat tccggctacg gtgatttcat ccgtatagac
      961 atcaacggtg attacggcga cctgggtgcg catatgtatg ccgttcgctg ggattactat
     1021 ggtgacggtg atactgttct ggctacctac ggcaccaagt ttgccgcaga caactggatg
     1081 cacaaggcaa tgggcatcca gctgggtctg accaagagcg accgctgcca gctgccggaa
     1141 ggcacagacg gctacggcag atgggatgtt accatctacg ctaccggata tcaggacttc
     1201 acattcacag tagacaatga gccggaaaaa tatgtatacg gtaccatgaa catcccgtat
     1261 gcggactact attacggtga actgggcaac agcacagata ccaccgacgg cgaaatttcc
     1321 tatgacgctg accttgccgg agatgctggt atgcgcgcag agggaaacta tgacgcagtc
     1381 acttctgcaa cagaaaaaaa atggaacagc atgtttgcag acaccacata tgtttctgag
     1441 ccggacgaaa acggcggcgg taagatcctg ggtgtttccg gcgtagaagt tgccattgag
     1501 cagagtctgt acgatcagct gactgctgac gcaaaggacg gcagcacatc cggcgttctg
     1561 ggcagcctgt tcagcacctt taccccgaac gaagaccagg aaacaattcc ggcagcatac
     1621 aagcagctgt ttgcagacgg caccctgtcc gcaaccatta ctgcagaacc ggaagttgac
     1681 ctgaccaacg taacaccgac actcaaaacc gacagcaagt acggtgacta ccaactgaac
     1741 atggacggtc tggatctggg agaaggcaat ctggtttacg gcgtgatcgt caagacaaag
     1801 accggcaact atggtatgct ccagctggaa aacatctgga agggcggcac cgagctggca
     1861 tggtccgccg gcttcaagac tacagaagca cacggaaaca gcctgcggtc tgctccgtat
     1921 actgcaacct ctggtgatac cattgtagag atcagctatc tgaccagcaa tggcgaatac
     1981 agcgtacatc tggcagatgg tctgtacctg ccgaaaaagc acactggtac ggttgccgca
     2041 gagagtgttg cagtgaccgc cggcgaaatt ccggtaacac tgaccgatct gccggaggac
     2101 tttgaggcag ttgtatccgc tgatgcactg accgatgcag cctatgcaga cggcaagctg
     2161 agctacgatg cagcaagcgc acagccgggc agctacacca ttaccgtatc ggacagcaca
     2221 ggcgtatatg caccggtttc tgccagtgta attctgacca ccgatgcaat gccggcagct
     2281 tatgacaagg acaagaacgc agttgttgct gcaaaggatg tcacggaaga agagcttgca
     2341 gcttatctga agaacatttc taaggtaacc atcggcgacc aggaatatgc tgcaaccggc
     2401 aagcgtggcg tgaagatcat tgaccccacc actggtgaga tcaacaccgc tgctgaaaca
     2461 cgtgacggcg ctgtctttgc aaagtctggc acatacaagc tgaccgtaga ggcaaccggt
     2521 tacaccacac tgctgacctt cgagattacc attgccatag cagacaacag aggcgacatt
     2581 gacctgaacg gcaaggttga ctccaccgat atgttctaca tcatgctgta ctgcgcaaac
     2641 atttctgcca gcaacaagga cttcgcactg agcgaagatg ctgctgaaaa caaaagaatg
     2701 atcgctgcaa cagacgtaga cggaaacggc aaggttgact ccaccgacct gttctatgag
     2761 atgtactaca tcgcaaacag aggcgcaggc aatgacgtga cctgggaaga tatcatcaag
     2821 taagcaaatt tcgataatcc tctgaaccat aaatgcagaa aaccccgggc agtaatgtcc
     2881 ggggttttct gtggttgata tgctttttca ctgtttgaaa ctatgggtgc aaggaatcgt
     2941 gtgcaattct gaaaggcatc ccttgacacc ggcaaaaaaa tcccctgcac tgctcttttt
     3001 acggagcggt gcaggggatt ttcttataat atgtttcgtt cttttagatt cctgccagga
     3061 tcaataccgc agccaggaca actgcacgca gcagggtgaa ccagatctcg ttgccacgga
     3121 agttccggat gctgctcttc gcatttttct tcggacagat ctccaggcac ttctgacact
     3181 ggaaacagtc gccgcttaca ttccagctgc cgtcctccgg cagggacaga tccgacgggc
     3241 ataccttttc acaggcagta cagcctttgg cgcatttttc ccttttgcgc cgcaccgaga
     3301 aaaacggcag aatgggcagc agggagaaaa cagcacccat aggacacagg aaacggcaaa
     3361 agaaacgctc acagaatacc attcccacca ggatcagcac cagcagaatg cagcccagcc
     3421 aatatgcgcc cactttgaag tttccggagc gcagcatgga gaacacatcc cagggactcc
     3481 agcccgtaag atctccgtaa acgcccagaa agcacagcag aaccactgnn nnnnnnnntc
     3541 agcaggagcg gtttcttttt gcacttttta cagaggaaca gatacaagct atgcaccgca
     3601 tcccccagcg ttccgaacgc acaggcataa ccgcagaaaa aacgtccaaa cacaatcgta
     3661 taggcacaca gcgccagcag cactgtcaga aacgaactcc atgcaatggg atttcctgcg
     3721 ccgatctgcg tcagaaccga cttgatgccg gaaaaagcag tagtaaatgc cgagggcaga
     3781 aacaggaaga aaaccagctg tatggctgcc cgcaggtagg catggatttt tttgggggta
     3841 aagtattttt tattcatgct gcgccgcctt tcccaaagca tcctcgactg cgccgagaat
     3901 gccgtttgaa ctaaaggttg cgccgctgac tacgtctaca tcggtagact gcatctccag
     3961 gatctcatca atgacaccct gtgcagcctc aaagtatgcc gcatcttcct tttcggcgga
     4021 aaccactgta cagtccgtga tctgaccgtc tgcaatggta acttccatct ttaccgggcc
     4081 gccgaaacct ttcgcctctc cggtgtaaac accgtctgca tagggcgact ccgtttctgt
     4141 ctcgctctcc tgcatcgccg cctgctgttc cgcaagctgc tttttggcgg ccgcctcgtc
     4201 tgctttgcgg tcactgacaa cggcatgata agcagatacc agcgagatca ccagcaccac
     4261 gttaagtgcc tttactgcaa atgtatgcac cgccgatcac gctcctttcc gtgcctgtgc
     4321 cagtgcatca tccacggcac ggacaattgc gtctgacgaa caggttgcac cggagacagc
     4381 atcaatgccg tctgtatgct gcttttccag aatctgtgca atggtacctg tgattttgcc
     4441 ggtacctttt gccgcacggg aaatatagcg gtcgttcagc ttatcgtagg aatcccctgc
     4501 accgctggca tctgaaatgc tgacgatctg gtcgttctcg atcttcacct tgacggacag
     4561 cacatattct acaaagtcct cgtcctcatc cgggtaacaa tccgctgttc ccacataagt
     4621 gccgtcgtcg taaatcttct cgaccggctc tgttgttgtc tccgtgctgg tcgtgccggt
     4681 gacgggtgca gctgtggtat tttccggcgg tgcaatggta gtgacagttg ttgtagtagt
     4741 caccgcaggc tgtgccgcat ttttggcagc atccaacgcc tcgttgattg cctccagaat
     4801 gccctctgag ctgaatgtcg caccggatac tacatccaca tcggtggact gccgctggat
     4861 caccagggaa atcagtgttt ccgcacgttt gaagaaggat tcgtcatcct cgttggatac
     4921 cacatgaatc ttttcgatcg tgccgtcttt cagcgtaagt tcaattttgg tgatgccgcc
     4981 gtagccctcg ccgctgccgg tgtatacccc gtccggatac ggaaatgcgc cttttttccc
     5041 cggttttgcc ggagcagtgg tctgttccgg tttcttttcg gttacagcag ccggcgtagt
     5101 ctctgcgctg cctgttgccg catttgccag cgcattccgc accgcctcga tcaatccggc
     5161 agaactaaag gttgcaccgg aaactgcatc cacattggtg ctttgcgccg caatgatgtt
     5221 ttgcagcagc gctgaggcat tttgcagata agggctgtca tccttggaag agagaatctg
     5281 gatatctgtg atcctgccgc ccgatacagt gacctgcaca gtcatctcgc cgccaaatcc
     5341 ggttcctgtt ccggtgtaaa cgccgtcccg gtatgccgca ggctcttcca cggtctctac
     5401 agtcggcggc tcttcattgc tctggctttt tgccgccgcc agtgtaggca aagcgctgtc
     5461 agatgcgctg ctgccgccgg ccttttgcag tgcatcccgt actgccgaga tcagtcccac
     5521 ggagctgtag gttgcgccgg aaacggtatc gacattggtg ctttgcagtg caacgatctg
     5581 tttcagcagc gccgaggcgt tttgcagata gggcgaatcg tctgtggcac tgaggattgt
     5641 aatgtccttg atcttgccgt cctcgatcac aacctgcact gtgataggac cggaaaagcc
     5701 tgttcctgtt ccggtatatg ttccgtcttt ataagtgccg gactccgaca gtttctctac
     5761 cgccggcgca ggcgcttttg tggtaacagc ggcagttttc cgctccctgc gcttttccgt
     5821 tgtactggtt tccgttgatt tctctgcgct ctcgcctact gcatccagcg acagaaccgg
     5881 tgctgtgtaa tcacccagcg aaacagcgat acataccgcc aggactgccg taggcaaaaa
     5941 gcccagcgcc ggaagatatt tcttgggatt tccccgaaat ttcatgaaat ttcaacctcc
     6001 tgtgaatagt tagttttctc taattagtct aacataacca ggagaaaaaa tcaagaaaaa
     6061 atctgtgtac aatctttcga acattccgtt aaccccgaaa aaatggcata aaaaaacggt
     6121 ttgtgataca cattcacaaa ccgttttgca caaaggatac gacgtttatg ccgtttcctc
     6181 ttctgtttca ttttccttgt tgtccgaagg ctttttcaga acaggcgtca tgctttttgc
     6241 acaaattgcc atagtagagg cattgtgcag gaatgctgac gttgtcggag caatcacgcc
     6301 aaacaagccc agcagcagca gggacgagtt aaaggcgatg ataaaccgat agttccggtg
     6361 aatccgtgcc atcagctttt cgctcagtgt acggagcgtt gccagttctg tcagatctgt
     6421 gccacggagc gtgatatctg ctgcctctct cgcaatatcc gaggcatcgc tcatggctac
     6481 ggaaacattt gccgccgcca gtgcaggagc atcgttgatg ccgtcgccca ccatgataac
     6541 acggtgaccg tctgctttca gctgttcgac atagcggtgc ttgtcctccg ggagaacctg
     6601 tgcaaagcac tgggtgatgc ccagtttctc tgcggtcacc tctgccgcct tctggctgtc
     6661 gccggtcagc atgacaatat tggaaatccc agcccgtttc atataccaca gagcagccgc
     6721 cggatttttc ttcaatttcc gcctgctgtt ccggtgtgat ggcaacgtgt tcatcttctg
     6781 aaacgaagtg atggctgccg atcacagcac gctttccgtg gagtgtggtc gcaatgccat
     6841 gcgccacaat atactgcacc tctgcgtgtt cctcctggtg attgaggttt cgatctgccg
     6901 caccctttac aatggcacga gccacgctgt gcgggaaatg ctcctcaatg caggcagcga
     6961 tcttcaagat ctcatcctcc gaatagtgac tgaacgagat cacacgctcc agcaccggtt
     7021 ctgcattggt gagcgtaccg gtcttgtcaa agacaatgct gtctgcctcg gcaaatgcct
     7081 ccagatattt tccgcccttg acggtgatat catggtctgc cgcctcacga atcgcagaaa
     7141 tcacggaaat cggcgtagac agcttgatag cacaggaata atccaccatc aaaatagaga
     7201 tagcctttgt gatattgcgg gtcagcagca gtgtcagacc aaagcccagg aaactaaacg
     7261 gcacaatacg atctgccagg ttttctgcac ggctctggac acctgccttg aggttttccg
     7321 aattgtcgat cagctggatg atcttttgaa tcttggtgtt tccgctgagc gcacgaacct
     7381 gcactgcaat cgtaccctca tccacaaccg tgccggcgaa aacagtagaa ccctctgcct
     7441 tacggacact gagcggttcg cctgtcatag acgcctcgtt gatattcgcc tcgccggaga
     7501 caacggtgcc gtctaccgga attacgctgc cggtgcggac acgaatcaca tcgccgacct
     7561 ggagatctgc cataggaatc agcacatcgg tgtcatcctg taccagccat accttatcgg
     7621 tcttgatcgc caagctatcc gttaacgctg cctttgtctt tgctctggtg taatcctcca
     7681 acagcgaaga gatcgacagc atgaacatga tggttccggc agtcttgaag ttccgctgca
     7741 gcaggcaggc gctgacagat gcaccgtcca gcacatccac attcagctgt ccattcagca
     7801 gtgtcttgac ggcacgccac gcatagcgca gtcccttcca gagaataacc gcattggcaa
     7861 tgggggcagg caagattgca ttttggatgt agcgctttgc gaccagcttg accagatcgg
     7921 ttttgaagtc cgagtcgatt cgctccgaac tttcctccgg tccaagcgga atgagcattc
     7981 tggtatccag tgcagaaact gcacggatca ccttatttct tgcgccttct tcataataga
     8041 ccagaatacc gccgttttcg gcatgagcct ctgctttcct gactgccgga tgttccagca
     8101 tctgtttctg cacagcggtt tccagttcct tatcgaaggc atatgcgccg nnnnnnnnnn
     8161 cttcttcgtc aacttcgttg tcgatgcctg ccttttcctt tgcatcgcag cagatatcct
     8221 gtgcctcgtc cttcatattc tggaatgtag cctttgcatc gttctgcagc ttcatgccct
     8281 ttgccagacc agatacagcc agttcgcggg tcttctttgc agttatgatc ttcttgccga
     8341 ttaccatcgc agcagcaccg ccgacagcat accacagttt ctcattctta aagcatttca
     8401 acatagtaaa gactcctttt tcattttaat atttgccgct gcaagccatt ggagcttgcc
     8461 ggcttttcgc atctgaaaca gcagttttgt gcttttcaga ctataaacct agtatacacc
     8521 cagaattaac aattgtcaac gggtttgcag gaaacatttt gtaagaaaaa ccaactgctt
     8581 ttttgtgacg tctgtgtata atatatgaac attggatagt tcaggcgaac taactgtttt
     8641 gaatttatag acaactaacc gcaacatcaa caacaagttt cttctgtttc agtcgcattt
     8701 tgagaatttg caataagaat tgcgtgacat acacannnnn nnnnnaccgc agactgaaaa
     8761 aaattatcgg acaagtacag gcaattgacc gcatgatcga cgaggatgtc ccctgcgaag
     8821 atattttgtc gcagatcaat gccgcaaaat ccgccctgca cggctgcgga aaggttgttc
     8881 tggagggaca catcaagcac tgtgtccggg acggcatcga acacggcgat gcagacaaaa
     8941 ccattgaaaa cttcaccaaa gcggtagagc gtttctcgaa catgggctaa tcgcattctt
     9001 tttttcaaca tttcaaagca attctaaagc agtctgacga aaatcagact gctttttctt
     9061 ttgtgcttga caaacccata cccccatagc gtat
//
LOCUS       DAWENO010000076         8986 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3229_length_8986_cov_11.906281, whole genome shotgun sequence.
ACCESSION   DAWENO010000076 DAWENO010000000
VERSION     DAWENO010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_8986_cov_11.906281"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    727..736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6306..6315
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6663..6672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7695..7704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2279 a         2351 c         1841 g         2475 t
ORIGIN      
        1 tgattgaggc aaaagctcat gtaacacaag atgatctatt ttatctgttt taccttctaa
       61 cggcatgaac caataagtgc tcaaaaagtc tttaatttgt gggtgaattc taaattccag
      121 atcttcttcc agcgaattga agtcaatcct tttttcctgc aatttgggca gccattccaa
      181 ttcgtcaaac ttatcatttt ctgcaacgat aagcgaactt tgtctgtatt tatcacatct
      241 aggaaccctt gggagagttc cccaagtacg tctccacgac ttgttgttgt tttcgaaaaa
      301 gatgtcaaaa gcttctctca tgctcataac gaatttcctc caagagaaaa tctgcacgta
      361 gatcagacaa gcgagcttta ttcagcgttc ccttagtcca aaaaatcctt gaccttcttg
      421 ctgcggctgg ggtgacgcag tttacggagt gcttttgcct cgatctgacg aattctctca
      481 cgggtaacat caaattcctt gcccacttct tccagcgtgc gggaacgacc gtcctccaga
      541 ccaaaacgga gacgcagtac ctttgcctca cggtctgtca gcgtggaaag cacttcgttc
      601 aactgctcct tcagcagcat caaagaggct gcgtctgccg gagcaggtgc gtcatcgtcc
      661 gggataaagt cacccaggtg gctgtcctcc tcttcaccga tcggggtctc caaagatacc
      721 ggatccnnnn nnnnnncgga atacgaatgg ttcttgcctg gtcagcaatt gcacgggtga
      781 ttgcctgacg aatccaccat gttgcataag tagagaactt aaagcccttg gtgtagtcaa
      841 atttctctac tgctttcagc agacccagat taccctcctg gatcagatcc aggaactgca
      901 ttcctctgcc cacatatttc tttgcaatgc tgacaaccag tcgcaggttc gcctcagaca
      961 gacgcttttt cgcatagctg tcaccctctg ccatccgctt tgccaattcc gtttcttcat
     1021 ccggagaaag cagcggcaca cgaccaattt ctttcagata gatcttgacc gggtcatcaa
     1081 tggcgatgcc ctctgtggaa agtgtcattt ccagatcatc tgtcatattc agatcgttgt
     1141 cattcagcgt ttcatctgtt gcagtatcat ctacgatctc aatgttcagt tgttcgcagc
     1201 tattgataat agaggcaagc tgatcggtgt caaaatccag ttcatccaat acatcggtaa
     1261 tttctttggt tgtcagtttt ccgttggctt tgccctgttc catcagtgca tcaattgtca
     1321 tttttttctg ttccatagta ttgttcctcc tattttcacc cattggggtg ctgctttttc
     1381 tgaatcaatt tcagcagatc atcatttgtt tcgactgtct ccggcacgcc cggatgcagc
     1441 gcccggatac aatcctgcac ctcttccttt gttaccggca cttcctggtt tcgtgctgca
     1501 atgccgctaa gctttcccat ctcgccctcg gacaaaacat ctcccatgga tgacagcgaa
     1561 aagcttgttt gctgccgaac actttcctga aacgcattca gtacctgctg aaagagtgct
     1621 gacgaaaact gctccggctt tatttcatcc acgatccacg cagattcttc cggtttggaa
     1681 agaatgtaga acaaaatgcg ttcctctgct tttcgttccc gtaaatggtt tccggactga
     1741 ggctgctgcg gttccggacg aaccgtatgc tctataatat ctttccactc tttcgtcgcc
     1801 tccaaccgcc ttttactgcg aatggtacga tctacctgct gatgcagaac ttccgccgaa
     1861 atttcccact tgtttgcagt cctggacaag tacacttcac gttccagcgg attctggatt
     1921 tctgccaaca cagaaaccac tcgtttgagc agctgtactt ttccggattc cgtattggta
     1981 tccaaaccat tttcgcatcg atccagacga aaattgattg catcgccggc ttgttccagc
     2041 aaaagccgaa accgttcaga gccatacttt ttgataaatt catccggatc ctttgcacca
     2101 gtcatatgca ggattcgtga cggcattccc acttcactga aacgattgat tgctttctgc
     2161 gttgcttgct gacccggacc atcgctatcg tatgcaataa tcatttcctt tgcatactgc
     2221 gtcagttttc tcgcctgttc cggtgtgatc gctgtaccca gagtcgcaac cacattggaa
     2281 aatccggctt gattgatggc aattacatcc atgtaacctt ccgcaagaat catatgcgtg
     2341 gaagaactgt cctttgcaaa attaagtgaa aataaatttc gtcccttatc aaacaccggc
     2401 gtttttgccg tattcagata ctttggctga ctgtcatcca agactctgcc gccgaatccg
     2461 atcacagcac cacgggtgtc cacgatcggg aacatcactc tgccacgaaa ggcatcatac
     2521 aaattgcctt ttccggaacg atgggcaaca tttgcagcca gtagctcttc ctctgtaaat
     2581 ccctttttca aaaggtgatc ggtgagtgta ttccaactgt ccggcgcata tcccaatcca
     2641 tattttttaa tggtctccgg tttcagctgc cgggaaacaa aatattgcag tccacgctta
     2701 tcatttccgg caatcagatt gcggaaataa aaattcgccg tttcccgatt gatcgcaaag
     2761 attcgtgtac gcagctgcgc acgccgatct gcctcccggt cattcggcac ttgcagtccg
     2821 cttttttcag caagctgttt taccgcatca gtgaattcca gattttccat acggcgaacc
     2881 caggtaatca catcgccgcc tgcgccgcag ccaaagcagt aaaaactttg tgtatccgga
     2941 aatatggtac aagacggcga cttttcagaa tggaacggac agttgcacac ataatgccgc
     3001 ccacggcgaa tcaaattgac ataatttcct gcaatcgtct ccatgggaca ggctgcacgc
     3061 agctgttcca gaaaatcgtc actgtatgtc atatctgtat caccgcctta tttccagccc
     3121 ttcgggataa atagctcctg gtacaagtcc actgcatagc cgtcactcat accggaaatg
     3181 taatcacaca cagcacggtg tgcatcttct ttttctgcaa tagagagata ttccgccggc
     3241 aaaagggaaa catgggaaat aaaatacgta tacagcgttt ccaacagcaa tttcgccttg
     3301 ttttcctcct gcttggcaat cggattataa tacacctttt caaacataaa ggcgtgcagt
     3361 ttgtcgtgcg ccgcctgtat ttccggtgcc attccaatcg tatcagaacc atgttccact
     3421 actgcaccaa ccagctttgt aatccgcttt gttttggtat ctcccagaat ctccaccaca
     3481 tcctgcggca gttcttcttt ctgcaagatc ccagctcgaa tcgcatcctc gatatcatga
     3541 ttgatatacg caatgcggtc agccacacga actacattgc cctcttttgt atccgccaca
     3601 cgattggtat gacagagaat gccgttccgc acttcatctg tcaagttcag cccttttcca
     3661 tgtttttcaa tcaagtcaac tacccgaagg ctctgctcat aatgacgata tccatgggga
     3721 cagatccggt tgagaatcga ctctccgctg tgtccaaaag gggagtgtcc cagatcatgc
     3781 cccagcgcaa ttgcctctgt cagatcttca tttagacgca gcgcacggga aatggtacgt
     3841 gcgacctgtg caacctccag cgtatgcgta agacgtgtac gataatgatc gccggcaggg
     3901 gataggaaca cctgtgtttt tcttttcagt cggcgaaatg agttgcagtg cagaatccga
     3961 tcccgatccc gctgaaacgc tgtgcgatac ggacaaggtt cttccggacg catccggtgt
     4021 gcgtgtccga tgttcgtgca ggcattcgga gaaagatact gcaattcctg ttgttctaat
     4081 tgttctcgta tactcataga tatcacctcc gtaggaaagg cggctatatg attctgcgaa
     4141 aagctgttca aaacagcttt ctatttatat atacgcagga gtgtgcaaaa ctcctctttt
     4201 ttcgttttct ttcgaaaagg cagcttacag cctttttgct ttcgaaaagt ctgcatactg
     4261 caatccgccg gaaacaatct ccacctgtcc gctggtcaga tcaatcaatt cctgtttcag
     4321 cttgtcccac agcgtgtcac gcaccagcag tttcagttct acctgatctt caaatatcgt
     4381 atccagctgt aaaatatctt cctggggaag gtgatatgca atctttccgt acagtgcata
     4441 atctgtctga atcgaaaccg gataacacgg catcatgatc ttgatctgcg ccgcctcgat
     4501 cgcaagtgct gtgctgtgag aatacgcccg aaccagtccg tttgcgccga gcagaacacc
     4561 gccaaaatat cttgtcacaa cacaacagac atccgtcagc ccgttttttt gcagcacatc
     4621 caggatcggc attccggcag ttccctgcgg ctcaccgtca tcagaatagc gtgaggcatt
     4681 tccgtcacgc agcaggtaag catagacatt atgtcttgca cggcgatgct gcttgcgcac
     4741 ttcttcaatt gcttccacag cctcttcctg ggtgcgtgcc ggaaaaagct gtgcgatgaa
     4801 ctccgatttt ttctccacaa atgaggccga caccggtgcg gcaatggtac gatattccat
     4861 catgtcatct acctccacgt agacaaaaaa ggacggtaaa caaaataccg tcccttttcc
     4921 attgtcgtgt tcaaaaaata aaccagcctg gaaattagtc cagattgaaa cgcttcttga
     4981 aacgatctac acgtccgcct gtatcaacca gcttctgttt accagtgaag aacgggtggc
     5041 acttcgagca gatttcgacc ttgatgtcct tcttggtgga catagtttcc attacattac
     5101 cgcagtggca agtgatggtg gtcttttcaa aattcggatg aatgccttct ctcatggggt
     5161 ttcacctcct gtatctgttg tttgtatcaa cagcagccca tcgaccattg cgccgacagt
     5221 agtcctgtga atctctgatt ttctgcggca tagccgccac agtttcttat tatagcatag
     5281 aatgaaatgt ctgtcaagtc cttttttcaa aaaaatattt ctttttcaaa aaaatcgaca
     5341 agcttatcac ttccaacaaa ctccttgccg gaaacaatgc ggagttttcc gcttggaaaa
     5401 gtcaatgtgt agacagcacc tttttgttga accgaaataa gttctgccca gcggtagacc
     5461 tgctttttcc cgaaaatgcc gtgcttttcc acaaatgtat ccgtataaaa gatgcggtaa
     5521 ttgcgtctga acagtatgca gaaaacaccg aaaaggctaa tcagcagaaa cagaataccg
     5581 caagcgaaag aatcccaatc ggtcattccg tcagcccaga taacgatgct gagaatgcca
     5641 aacgccgcaa aacagataaa ccagatgccg ccgacaatac cgaaagcttt agaaactctc
     5701 acgcattttc gataagaatt ttcttcgttt tctctgtcga tgtattgatt ttcccatttt
     5761 tcaaattggc gagaggaaag ttttgacaac aggtgagaaa cagcatgaag aaacagggaa
     5821 accattatgc acctccaacg gattcaaaat atgcacaaag cgccgctggc gaaaaagctg
     5881 cattggtttc cagctgccat tccagttctt ccagattgtc cccgtcatat tcgatttcct
     5941 tgacgaaaag ctgccagatt cgcagacgct gttcttcatc ctgtattttt atgtgtgctg
     6001 taagagttaa aacagtacaa actgcaaatg cggcaagttg caccggcaat aggattgcaa
     6061 aaggatcatc cgaatatgcc gtccgaagat aataccgata cagcatatcc atcccaaaca
     6121 agcgtatttc cttatccaat gctgcatcgt aaaataatga cacttccgga ttttcccttg
     6181 cccgggtcaa aatcattttc caatcctctg tcagaacttc gcagccgttc aaaaatgaca
     6241 aaatatcatc ttcagtgcaa attgcatctg aggtaccttc cgagaaatcc gtccattgtg
     6301 tcaccnnnnn nnnnnggcaa tgcgatatct gtttcaatgc cggaacagat gtatcctgca
     6361 aacaggcaaa caagatgcgg cgctggtctt ttcgctgctg catgattttc ccgtcatact
     6421 ccggaaagct gcctgtcact tccggacaat cattttctga cagcagccat tgttccggcg
     6481 tcagttccat caacagccgt gccgcctccg gacaagcagg ggagatgtca tattcgatga
     6541 agtcaccgta gtcctgagtc actctgggga attgtctgca agtttcacac agcgccgctt
     6601 cacccagtcc tccgtgaata tcacagagct gctgcgcatt ccaaaaaggg cagcgtttct
     6661 cannnnnnnn nntcttgaaa aatcacatct ccgtctgcat ctgttatcat tgcctgacgc
     6721 aaacgatttc ccaattcacc ggaaaccatc tggtattttt ccactgactc cgcatccaca
     6781 acgatttccc attccttgca gcagctgtcc ggacaggctc cggcaatgca gtgaaatgcc
     6841 gcataatggg gtggatagat ttctctcata attcattcct cccgtcaaag gcattatagc
     6901 atggagtcag cgcctttgtc aactaccgta agaaacaaaa caaaaaggca ccatgctcct
     6961 gcacagtgcc tttttgttgt caatgtaaag tgaaattatt acgcattttc cagacacatc
     7021 tgacgcagtc cggttaaatc aaagccgttt acggtttcgt ccatgtccac atctgcacag
     7081 ctgtaagcat cctttgtcag agtcgtttca cccagcagat acttttgcag cagaacaaca
     7141 tccgcaactg taattgcatc gtccagattg acatcaccca gcagatgcag atccggtttg
     7201 tccggtccat ccgggtctgt taccagttct gctgatgcaa tcgaagaaat cagataaatg
     7261 gtcatatctg aactggcacg ggcaaacagg tgcgccgctg tactcagatc accccaggct
     7321 ttcgccattg actcgtgaga atagtacgca attccgttct cttcgtcaat gtcatacgca
     7381 ttgatttctg tccaattctt ccagttctca tcgcacaacg ccagaatcgg cgcagagttt
     7441 ccggtgtaac gaatcgcaat ttcccgatct tcgcccaact gttccggcgt cacggtcaga
     7501 ccaggcgtac agtttccacc gttgccgtct gatgcgatca aagacaatcc atccgcattt
     7561 tcgtagatga tgtccccgct gttgatgtca gcgtgtttcc aggtgggcag atggctctcg
     7621 ctgccccata caacactgtc atcattcatt accgccatca ttgcatctac aaccggctca
     7681 gatgcctcat accannnnnn nnnncatgta ttattgctgc gatccaaata tccgtgtgcc
     7741 tcagatgcag aggagtagtt ggtgattgcg ttgttgtccc acagcacgca ggggataccc
     7801 agtttctttg tccatgtcaa ataataagtc gcccagtcgc atcttgcggc agtgttgtta
     7861 aagttagatg cactgaattc gcccagaaca acagggatgt ccttatccgt gaaatagtac
     7921 cgaatgtcag agaacagcgt atccagatca ctctgataat tgctggagaa tgtagtgtgg
     7981 tcgccgtcgc ccatagcaaa cgcataaggc gtgtacgcat ggagcgaaac tgccacatag
     8041 tcgtcatccg gcacatccaa agaagaatag gaacttgcat aggcagatgc cgcatacgac
     8101 ggaatcatca ataaacgggt gttctgatac ggagattcca cagaacgcac tgtctctacg
     8161 aaatcgttgt ccagcttgtt gactacttca taacactctg cgttgccctg ccattcgtag
     8221 gatgcaccag tctgtctcgg ctcgttcatg ccctcaaaaa tcagatgctg gtcatagtcc
     8281 ttgaaatacg ctgcaatctg taaccacatc tgtttcagcc gttcggacat ttcatcataa
     8341 gcagtgggga agtcggaacg attgatccac tcctcgtggt gcagattcag aatgacatac
     8401 atatcctgct cgtatgcgta atccactact tcctgcacac gttgcagcca cagctcactg
     8461 attgtataaa caggcttgcc gttttcgtcc gttgttgtcg taacattcgg ataccaggta
     8521 gtcggaattc gaatggtgtt aaagcccttc gccttcacag catcaatcaa ttcccgtgtt
     8581 actgtcggat taccccagct ggtttcctgg gatgaatcgt atcccgtagc atccagggaa
     8641 ttaccaatgt tccagccaat ggtcatattt tgcgtgatat catctgccgt cattacattt
     8701 tctgctgaaa ccccgaaagc cgggaaaaca gctatactgg aaagcagcat caccgctgtc
     8761 gttccaacgg taaagatttt atggaattta gattgcattt tgtcctcctc atttcacact
     8821 tcaaaaacac atcaaattaa aatgtaccag attacagtac atattttatt atatcacagc
     8881 aggcggcaaa agtcaacaaa atattttctg ttacaaaaaa ctttttgtac aagtttttga
     8941 tcatagaaag caaaaagaga tacggaacat ttctccgtat ctcttt
//
LOCUS       DAWENO010000077         8840 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3288_length_8840_cov_11.617188, whole genome shotgun sequence.
ACCESSION   DAWENO010000077 DAWENO010000000
VERSION     DAWENO010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_8840_cov_11.617188"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1964 a         2518 c         2076 g         2282 t
ORIGIN      
        1 acataatcgc cgtctttgca agactttccg caaaaaaatt tacgtttccg ccggaaaaat
       61 cttacagcac cgtcagctgt ttcggcgcct cggtcagatt ctcgcagccg ttttcccgaa
      121 tgaccacaaa atcctcgatg cgcacgccga attcgcccgg caggtaaatg cccggctcta
      181 ccgtgaccac gttcccggtc tccagctttg ccagcgttcc cggcgaggca ttggggaact
      241 cgtgaatttc cagacccacg ccgtgtccca gggaatgccc gaagttgtcg ccgtagcctg
      301 cctcttcgat gatatcccgt gccagcttgt ccatgtctct gccggtgatg ccgggttttg
      361 cgcccacaat gccggcagcc tgggctttca gcacgatatc atagacctgc tgcattcttt
      421 cgctgggctt tcccacgcag acagtacggg tcatgtcgct gtgatagccg tccaccatag
      481 cgccgtaatc catcagtacg aaatcgccgc tctgcacgat acgctgcgca gagggtacgc
      541 cgtggggcat ggaagtggca ctgcccgtca gcgcaatggt ggggaaagat tccccttctg
      601 aaccgttttc catcatggtc tggttcagcc gcagcatcac gtcacgctcc gagatgcccg
      661 gacgcaggaa attgcagata tccgcaaagg caatttccgc aatgcgctgc gccctgcgaa
      721 ttttgtcgat ctcctcttcg ctcttcaccg tgcggcacgc atagatcaca cggctcaggg
      781 tatcgctgta atccagccgc accagattcc ccagctgctg tttcagcttg gagaactggc
      841 tcagcgtcat ttccatcgcc tcgacagaga cggtctttgc gctgtgcttt tggaacagtt
      901 cccggagctg tgcaaacagg tcgtgctgct cgataacggt acagtccttc acgcattccc
      961 gtgccttttc aatatagcgg aagtcgatga taagataggc ggcatcccga aagcagacga
     1021 cggttccggc agaggactgc atcccggtga aataccggcg gtttacatca gattgaatga
     1081 ccgcagcgtc tgtctccggc ggcagctgct gcatcagttt ttccaaacgg ctttgcatga
     1141 tgaaaacatc tccttacatt tcagaaagtg cctggagcgc caggaaatag ccgttgatgc
     1201 cgaaaccggc gatcgttccg gcgcagaccg gactcagcac agagtgggaa cggaactcgt
     1261 cccgtgcata aatgttgctg atatgtactt ccacacaagg gatccggatg tcgtgaatag
     1321 catcatgaat ggcatagctg tagtgggtat atgccccggc attgatgaca acgccgtcaa
     1381 aggtattccg tgcagcgtgc agcttgtcga tgatcgcacc ctcgtggttg gactgaaaga
     1441 tctcgcactc cagtcccaga ccctctgcac gatccaggat catccggttc agatcagcga
     1501 aagtggtatt gccgtaaacg cccggctcac gctctcccag cagattcaga ttcggtccgt
     1561 ggatcaccaa aactttttgc atcatagcag ttcagctcct tacttttctt cgtatatttt
     1621 cttcggcaga aacggaatct ccatccgccg cttgaatttg aaaaacttcg tcttttccat
     1681 ctccatgggg aacacaaaga tacagggcac ccgaaacagg tttgcaccca gcacatccgt
     1741 gaagatctgg tctcccacgg cggcgacctc cttccgggac agccccatct tttccactgc
     1801 ctcccgatag cccttggtca gcggtttctt tccctcacag acaaagtcca gccccagccg
     1861 ctctgcgaat ggtgcgaccc ggggcggatg gttgttggac accaggcaca gcgaaatgcc
     1921 tgccgcctgc attccggcga cccatgcctc gacaccgggt gccggcacag ggttgtcgtg
     1981 ggtggtcagt gtgttgtcta tatccagcag caatccccgg acgcccagtt tttccagaag
     2041 atgcggtgta atatcgcata cgctgcgcag tgcgattttt gcatgaaaca gcatggctgt
     2101 ccctcctttc ggcagagcgg tgcagaccgc agttacatta tacgcctttt tccctgccgg
     2161 aaatgtcgaa cgatggtcgt tcatttacaa aaaacgggcg cactctttga aagtgcgccc
     2221 gcctgctgtg tttcgcaata cgatttatca atacttgcgc ttacgagcag cttcagactt
     2281 cttcttacgc tttaccgaag gcttttcgta atgctctctc ttacgaactt cagcgataac
     2341 gccgtccttc gcacaagatc tcttaaaacg cttcagagcg gtgtccaaag actcgttctt
     2401 accaacgcga acttctgcca cttacatttc cctccctttg ccgctacggc agaggctaat
     2461 cttccgccag accatacggt cggacttccg atctatacca ccaataatcc ccagtcggga
     2521 acatcgggta tcagcttcac ctatttcgct gcaaactatt tttgcaatat aaaacattgt
     2581 accacatatt gcagaatttg tcaagacctt tttctgcaaa cttagaaatt tgttatttta
     2641 ccacaaaatt cacaagtttc tttggtacat atatttgctt aatgattgtt ttgcctgctg
     2701 tggcttcttt tatttttgca tcagcggctg cctgtgccag aacgtcatcc ttttctgcat
     2761 ctgccggaat ctccagcttt gcctttacct tgccgttgat ctggacaacg atctcgatca
     2821 tatcgtccac gcacagcgcc tcatcatagg tcacccattc ctgctcgctg agaacaccct
     2881 cgcagccgat cacctggtac agctcctcag agatgtgcgg tgcaaacgga tacagcagga
     2941 tcagcagatc acggaattcc ttcttggtga tcgagccggc atcgtagata tcattcagca
     3001 gagtcatcat tgccgcaatg gcagtgttga acttcattgc ctcgatatcc tcggacacct
     3061 tcttgatggt cttgtgcatc ttggagcgca gggcatcgct gtagctgtcg ctgtcggtca
     3121 tcttgtcctg gagcgcccag atacggtcga taaagcgctt gcagcccttg acactgcttt
     3181 cgctccacgg cgcagccttt tcatagtcac ccatgaacag cacatacaga cgcagcacat
     3241 ccgcaccata gacatctacc acatcattcg ggtctaccac gtttcctctg gacttggaca
     3301 tcttttcgcc gtccggtccc aggatcagtc cctgtgcggt acgctttgca tacggctccg
     3361 aagtgggaac aagatccagg tcatacagga acttgtgcca gaaacgggag tagatcatgt
     3421 ggcgtgttac gtgttccata ccgccgttgt accagtcgac cggcatccag tatttcagtg
     3481 cctcctggga tgcaaattcc ttgtcgttat gcggatcgca gtagcgcagg aagtaccagg
     3541 aagagcctgc ccactggggc atggtatcgg tttcccgtct tgcatctgcg ccgcacttcg
     3601 ggcatttgca gttgacaaag gattcgatct ttgccagcgg gctttcgccg tccttgccgg
     3661 gttcgaagtt ttctaccggc ggcaggcgca gcggcagctg gtcataaggc accggcacca
     3721 tgccgcactt cgggcagtat acaatcggaa tcggctcgcc ccaatagcgc tgacggttga
     3781 acgcccagtc cttcatcttg tactgtacgc ccttttcgcc gcagcccttt tctgccagca
     3841 cttccagcat cttttcgatg gcatcctttt tgttggagat gccgttgaga ataccggagt
     3901 tcaccatttc gccgtcgccg gtatatgcct ccttggagat gtctccgccc ctgataactt
     3961 cgatgatatc aatgccgaac ttcttggcga agtcatagtc acgggtatcg tgtgccggca
     4021 cagccatgat cgcaccagtg ccgtagccca tcataacata gtcggaaata taaatcggga
     4081 tctccttgcc ggtgatgggg ttgatgccgg tgagaccttc gatcttcacg ccggtcttgt
     4141 ccttgaccag ctgtgtccgc tcgaactcgc tcttcttggc gcattctgcc ttgtacgcat
     4201 ccaggtcagc aatgttggcg atgctgtcac gatacttctg gatgatcgga tgctccggcg
     4261 caatgaccat gaaggtcacg ccgtacaggg tatccgggcg tgtggtatag atgcgcagct
     4321 tttcatctgc gtgttccttg atggagaagt tgacaaacgc accggtggac ttgccgatcc
     4381 agttctgctg ctggagcttg acgttgggca gatagtctac ctcttccagt ccctggagca
     4441 gcttgtctgc gtattctgtg atgcgcagat accagacctc cttggtcttc tggacgatct
     4501 cgctgtggca gatgtcacac tttccgccct gggaatcctc gttggacaga accaccttac
     4561 agctggggca gtagttgacc agggtcttgt cccggaaagc cagtcccttt tcgaacatct
     4621 tgaggaagat ccactgtgtc cacttgtagt actgctcatc ggtggtatcg atcactctcg
     4681 accagtcgaa agaaaaaccg acacgcttga gctgtccggt gaattttaca atattgtcgt
     4741 ccgtcacctt acggggatgc acaccggtct tgatggcggt gttctcagtc ggcagaccgt
     4801 aggcatcaaa accgatgggg aacagtacat tatagccctg catccggcgc ttgcggctga
     4861 caacttccag accggaatac gccttgatgt gtcctacgtg cataccgtga ccggagggat
     4921 acgggaattc taccagcgca tagaactttg gcttggtgtg gtcttccttg gcacggaacg
     4981 aaccgtgttc ttcccagtac ttctgccact ttggttcgac agaggcaaaa tcatatttgt
     5041 tgctcatgta tttatcattc ctttaattat aatttactca acatacttcc aaggctgtga
     5101 cgccgtttcc ctcaattttt cttcgatact gtcacagctc catgtttcat tcaggtcaaa
     5161 ccggcatctt acacgaacac gactgtcctc tccctcaatt tcccgtccgg catggtaaaa
     5221 cttcacataa attgcgtatg tggtctgatg cgcattgtcc ggttcggtac tcagatgaga
     5281 aaacggcgac gtatcccaaa aatcacagta aagtgcgtag atctcttctg ctgtttcaga
     5341 aaagctgtcc gacgtcatat caaaagaggg attcccctca ataaaatcaa agtcaagatg
     5401 ttcactctcc tgcggtgtcc cgaatgccat gacaaattct tctgttacca tcgtattgat
     5461 ttgatgctcc tgacaaaaag ccgcccaggc agctgtctta taattctcac gcacactttt
     5521 gtgcggaaaa tgccagattg gcagcaaacc atcccaacgg ccctttgacc aaccggatat
     5581 gacaataaat accacatctg gattttcttt cggtgaaacc gtataaaaag tatagattcc
     5641 gctgtctgtc ttttgaatat ggctatcaca aatcgtaaac gcttccccat attcatcttg
     5701 tagctgacgc attatctgcc atctgccgtc cggtaaaaga aatttgacaa gcactgcgat
     5761 ccaaaagaca agaaccacaa aaagtattcg ccattccgtc tttgactttt tcggtttccc
     5821 gctgttattt accaaaaaac gccctaacat tcttttcaaa taccaacact gcagcagcgc
     5881 ccagatccag cagaccctcc aggaggtaga tccactggct gcccagattg ctgaggtttg
     5941 cgccaatgtg ggtcagaaac atgatcgcca ggaaaacggc gacaatgtag ttgatgtacg
     6001 gcacacgatt cagcatcagc accagcgcaa ccacgccgac gatcagcatg atgatgttgg
     6061 agacgctgaa tcccaggatc agattcagaa cgccctttag aatcagataa agcccgatgc
     6121 caaataccca ttttttaccc atggtttcca tacggcagga tcctttcttc tatcgttatt
     6181 tctaaaatcc aatcgccgaa ggcggcatta gatttttttg atgcagctat ttttctaaaa
     6241 aaatgctgcc gaggtccagg gcgagaagcc ctggtcgctg tccgcagaca gcgaaactct
     6301 ctgcgccata cttttggcgc aacggagaaa agggtgagaa aatcgacaga ttttcgaggg
     6361 gaaacgaaca cgaccgtttc ccctggctgc ccttttgaaa aaagcttacc cttgaaaatg
     6421 ccccagtggg gcattttcaa cgctctaaac tcagtccggc ttgacccact gcgaaatatc
     6481 caccggctct gcatctgccc agaggcgttc cagctggtag aattcccggg catccttaca
     6541 gaacacgtga acgatcacgt cggcatagtc cagcacgacc cagttcgagc cgttgcctct
     6601 gccctcacgg tgcatcggct ctacgccttt ctctgacatc tgatactcga cctcatccgc
     6661 cagcgtccgg gtatgtgtgg tactcgtacc gttggcaatg atgaagtagt cgcccagaat
     6721 ggtcagatcc tgcacacgaa gtacctggat atcctctgca cgcttgctgt ccagtgcctt
     6781 tacaatagct tccaaaattt tctgctgcgt catagttgtt cctttctcaa agcctgcccg
     6841 atgacaaagc acgctcttat ttactgggcg ccgtcattgc tgtcgccctt gctgatatcg
     6901 ttcagatttt cctgggtgtc gtcatagtct ctcgccagat attcgccctc cggcacaagc
     6961 tccacgatgg cgctttcctc cggatagatc tcgttctggt acggacggaa atggccgttc
     7021 agcaggtcga tggttgccga tttgtgaatc gaatacaccg actgcgcaga gccgtcgccc
     7081 ggataatagg tcgaaccctc acccggcacc ataaagacat tgacgttgtc catagtcagc
     7141 cgctgggagg caaagtctgc caccatgctg atctgttcca gagagaggtc tgttgcgatc
     7201 cactggttct tgtagatctt ctgcatcgca ctcatcagtt ccgtctggct catgttcagc
     7261 atcttttcca tggctgccgc caggaagata cgctgtgcct tgacacgtcc gatatcacct
     7321 tcggcatagc cgtggcggaa acgcacgaac cactccgact gctgtccgtt cagcgtctgc
     7381 tcaccggcag gcagcacctt gtctgcctca taggtgatct gctccggcag tgtgatggga
     7441 atgccgccta cagaatctac aatattggcg atatccacac agttcagctt cacataggca
     7501 tccacataga tgcagaaatt ttcccggatg gcatccacta cacgctggat gggcgtaacg
     7561 ccggtctcct gcccgctgtg ataaatgctg ttgaacttcg tcgtggaata ggacgcatag
     7621 tccggcatat aggagtcacg gggaatctgg agaatattca tggagccggc gatcagatca
     7681 aactggagta tccagtctac gtcagacagc tcgccgctct cgtccatgcc caggaacagg
     7741 atagtatact gtccctcgat ggtctgttcc agatccaaac catcgttcaa attggaatcc
     7801 agatcgcttt tcagattcag cgccgagtca ttggaatcca cctttgtcag cgtaccctct
     7861 ttttccacca gcggctgcca caatttccag tacttcataa tggagacctg ctgtgtggta
     7921 atggtatttc cgtcaacttt cttgaaattg atgatcggtg cattgaacac catgagcaga
     7981 atcagcacca cgctgatgac aatgcctgcc accattgtga taatatcccg tgggctgttc
     8041 tttttccgct ttttgggtgc atagtatccg tattcatccg agtggttttt tctgctcatc
     8101 tgcaataatc atccttcctg ccggtgtagc ttgttcaaaa tttcgttgta tgcctctacg
     8161 gtgtggtgcg ggatcagtgc atcccggcgc accagtttca tcagagaata tttcagtgca
     8221 tagcccatgc cttcatccaa actttccagc gcctttttcc gcatggtctc cacgtccgga
     8281 tagtcccgtt ccacggagat catatctgcc agatagacga tcttttccag gtcggacatt
     8341 cctgctcttg cgatggtgtg ataccgcacc gccgaaagca catccatatc catcacgccg
     8401 aattttgtcc gcagcagctc cgctccggcg atgccgtgcc agaccttgaa cgcactgcgt
     8461 tcctctgcgc agacatccat cgcagactgc atcatcagcg tgtactgcac atcctccggc
     8521 tcttccttgc agatgtcatg caccagcccg gcaaaccgag ccttttccac atccggatag
     8581 ccgaattttt tcgccagcat cacactggca tccgatacat tcttcgaatg ggtataccgt
     8641 ttctgagaga gatgttcttt cagatattcg tcatattttt ccagctgttc catgtataaa
     8701 agcagactcc tttcttcgga atctgtctgg ggttcgtttc cagcagatca cacaaaccgc
     8761 ccgtccgaaa tcccggacga acctctaggc aacaccgcac aaagcttgtg cgtaagatgc
     8821 gaagccggat ttttcggcag
//
LOCUS       DAWENO010000078         8788 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3314_length_8788_cov_10.014657, whole genome shotgun sequence.
ACCESSION   DAWENO010000078 DAWENO010000000
VERSION     DAWENO010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_8788_cov_10.014657"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    455..464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1789 a         2392 c         2428 g         2169 t
ORIGIN      
        1 tggtaatcag atcgccgctt ttcagcgtgg tgtctttctc caggtagatc atccggcagc
       61 ggtattctga tgccagcgtc acgtcaccct ccacaatgcc ggattccggc ttgctggatg
      121 cttttgcgcc aatggaaaga ttgggggagc agacagtttc tactgttgcg taggtgtcgg
      181 aaatttcgga aataataccg acaagtcctt ctgccgtcac aactggatcg taaacggaga
      241 gtccgtcatc gctgccccgg tcgatgtaaa agccgtggaa cgggtcgttt tcgacatagc
      301 cgatgatggt gcaggggtcg gagaactcgt tgtccgggtg atcctctttg atgcccatga
      361 actcggacag atccgccaga tcctgtttcg tcttgtcata gtccaccatc tggctttcca
      421 gttccgccac acgctgacgc agctcctcgt tttcnnnnnn nnnnccggaa gaaatgctgt
      481 tggagacgga acgaatcgga ttcagcaccg ttcccagaaa gccggtagtg ggcagggtat
      541 agccgtccct ggttacggca tagagcatca tgccgatcag aagggcaacg atgcacagaa
      601 tgatgcgaaa ctttttgctg cggaaaaaac cgcccatgtg gaatcagcct cctaccttat
      661 tcaaagcctt tggactcttc tgaaacagca tcccggtatt tgtccagatt ttccaggatc
      721 tttcccgtgc cttctgccac gcagtccagg ggatactcgg cgataaagac gggcattccg
      781 gtctccttgt ggatcagctg atccagtccc cggagcagtg cgccgccgcc ggtcagtgta
      841 atgccgtggt cgatgatgtc agccgccagc tccggcggtg tctgttccag ggtacacttg
      901 acagcgtcca cgatccggga cagcggctcg gcgagtgcct cccggacttc tctggaggtg
      961 atgttgatat tttcgggcag tccgctcatc agatttctgc ccttgatctg catcgtttcg
     1021 tcacggtcca ccggataggc actgccgatc tgaattttaa tactctctgc ggtacgctcg
     1081 ccgatcagca ggttgtaccg tttcttgatg taattgataa tggcagcatc aaactcatcg
     1141 ccggcgcagc ggacgctctg agagaccaca atgccgccca gagagatcac agccacttcg
     1201 gatgtgccgc cgccgatgtc cagcaccata cagccggtag gctctgtggt gggcagtcct
     1261 gcaccgatcg ccgctgccat cggctcttca acgatcagca ccggctttgc accagcctgg
     1321 tctgcggcat cccggactgc acggcgttct actgcggtca cgccggaggg aatgcagata
     1381 atcactctgg cacgtgcgaa cattttgccg tgcattgcct tgcggatgaa ctcctgcagg
     1441 agaatcgtcg tggtctcaaa atcggcaatg acgccgtctt tcagcggacg cacggcaacg
     1501 atcgagccgg gcgttctgcc gattacttcc tttgcctctt ttcccacgta tttggcacgg
     1561 tcggtgtggg tattgactgc gacaacggat ggctctctga tgatgatgcc ctttccacgc
     1621 agatatacca gcgtatttgc agttccgaga tcaatgccga tatctttcgt aaacataatg
     1681 tatgctcctt tggatgcagt tctacacgct gcatcattgt ccccagtaag tcatttcttt
     1741 catgagattt gcggaaaatt tcggtgactg gctccaaaaa ctgagccgga aagcgccgat
     1801 ttctgcactt ttcaccatag aaaagagaac actgctgccg aaagatccgg tgcagtgtgt
     1861 ccggttgcat gaaattttcc gctctgatgc agactgctgc gctttcctgc gctgcggcag
     1921 ccttttgcat cagacctctt ctattatacc acgaaaaaag cagggtggca atcttttttt
     1981 taggtttttt ctgcgaattc aaaattctat catattgtga aaaatcaaca agttttaatg
     2041 caataattac agcaaatata acaaacgctg tatggagcaa tgcgagacgc caagcggcac
     2101 tgtaccggaa ttgtccggtg ccgtcttgag ccttattact ttttgccgat cagcggttcg
     2161 atcttcggcg caacgaagat ggcaacgatg gtcagcagga gctgcgcaat gttaaacttg
     2221 gactggatgc cgaaggtgag atcggcaaca tagaggttcc aggtgtggct tttgaggtgc
     2281 agcggcggcg catagtccag ccagctgagg ggagcgatgc cggcgcagag attgccgatg
     2341 acgccgccca ggatcactgc gaccacgatg gagcagagat agacgagaaa ctttttcata
     2401 gacggggaga ctcctttcgt ttacgacaga ccgcttgcgc cgaatccgcc tgcgccacgt
     2461 tctgttgtgt ccaggcgctg gacttctttc agttccgggg tgagaatggg catgatgacc
     2521 agctgtgcga tgcgcatacc ctcttctacg gtaaacggct ctgtgccgtg gtttaccagc
     2581 ggaatcagca gttcgccacg atagtcgctg tccaccagac ctacactgtt aatgagtgta
     2641 atgccgtgct tggtggaaag tccggaacgt gggaagatgc acagggcaac atccggacgg
     2701 gacggagcaa cagcgattcc ggtcggcacc ttgcagatct cgccgggctg aatggtcagc
     2761 ggcgattccg gcagggcata cagatcgtac cctgcgctct gcgctgtggc acgcttgggg
     2821 agctgtgcgt gagggcgaag tttctgaaca gataagtaca taaaataact cctttattca
     2881 acaccacgga aaaacaagcc ccgggtgatc gtttcgtttg cagttttgcc gctgcggtag
     2941 gcgtggagaa cagacagcac gtccggctgc agtgtgcagt ccagcaccag gaaatccatg
     3001 tgggaaaatg cctccggctt gtccgccatc caggtgggga cagcgttgta catggtcgtg
     3061 attccggcag gtctccggca gtataccggg aactgccgac cagttcggtc aatgagatgg
     3121 tgggtgcagt gcttgcagcc aacctctgcc tgaatgggac aattccgcat ggttatgacc
     3181 ggctggtatc cataggcata agcgccgacg ggcagcccgg tgcaggtctt ggtctgggcg
     3241 atggtcagct ccggggacaa aatcacatcc tgcagggatt cttcctgcaa aaatgccaga
     3301 cagtccggat tggctgcgtt cagtcccatg ccgccgtgca gtgtcaagcc cagctgtctg
     3361 ccgatctgca aatgagagac gttctcgcag acaatgtggg agaatcccag ctgttttgcc
     3421 tgttccagcc agcccttcac ttttgtttcg tccgcacaga accggggcag agtcagatag
     3481 actcgttccc ggacatttgc tggaatctct gccgatgccg tttcgacagg caggagcagt
     3541 gccgcaacat ccggctcatc cagcagctgt gcggaaagcc cgttccagtc cggctgaatg
     3601 atgcggaact ggcgctgttt tgtgccgcag gtctgtcctt tggacagggt gacaggctgt
     3661 atggtataat gaggtgtgtt ggcggcaatt cgctttgcat ccattgcggc gatgccgtcc
     3721 cggcgcagtg cattccaggc agatgccggg agcattccca tgccgtcgca gtctgcgtgc
     3781 agttctgcca gagcgtagat cgtaccgccc agctttccca gctgacgttc cagctgtgcg
     3841 gcatccgttg ctttggttcg tgcaggctgt accgtttcgc cggagacggt gacgcagttc
     3901 ccgtccgcat cccggagtgt cagagctgcc ggctcgccgg tttgtacacg gacatccata
     3961 gtcagcgcaa tctgcggtgt tggcttttgg taaagctgtg ccagatgtgg caggacttcc
     4021 tttgcctgta acacgtcctc tttctgccgg gtgccgaaca tctgctgccg ctttccggtg
     4081 tagtatccgt ctgtgaaacc gctccgggag aaaaccgcac gcagggtgtc caggtcgggt
     4141 tcttctccgg ctcttgcctg taccagagca gtgactgcgg cggcaacgta ttccggccgt
     4201 ttgcagcgtc cctcgatctt cagcgatgcc acgccgtcct gtgccagttc tgccagatac
     4261 ggcaccaggc acagatcttt cagcgaaagc gcacacacgt tttttgctcc ctttgcagaa
     4321 aaggggagac ggcacgcctg ggcgcatctg ccacggttgg cacttctgga gcctatcact
     4381 gccgacagac cgcactgtcc ggaaacgctc atgcagagcg ccccgtggac aaaagtctct
     4441 acctccacac cggtctgaca gactgcggcg atctcttctc gggaaagttc ccgggagact
     4501 actacacggg agatgccgtg ctgttttgcc cagtgtgcgc cctccggcgt gtgaatcgtc
     4561 atctgggtgg atgcgtgaac ggggacatcc ggggacatcc ggcggatcag ccgcagaacg
     4621 cccagatctt gtaccaggaa cgcattcacg cccatttcca ttgccgcctg taaaaattgt
     4681 accagagagg aaaactcaga ctcgaagatc agcgtgttca ccgtcagata gagctttgcg
     4741 ccgtagagat gacatagttc cacagcgtgc tgtaactgcg gcaagtcgaa attggctgca
     4801 ttctgccggg cagagaacag ctttccgccc acatagacgg catcagcgcc gcagcgcagt
     4861 gctgccagca gagattcttc cgagccggca ggggctaaga tctcaggcgt tctcatttgg
     4921 catttttctc acgcagttcc cgttccagtt ctgccacacg ggatttcagc acctcgacct
     4981 gctgcatggc tgcgtcacgt tccagacggg tctttccagc ctcgtccaca tattccttgg
     5041 actggtcacg gatgctgtcc agcttggcgt tcgccttgtt cagatcatcc agtgttgcca
     5101 gccccaccat gatcgccgct gtaaacggag aggcgctgct gttggcatcg gtgatctcag
     5161 cgatgcggga ttccagtgtc cgggcaagcc caaagacata gttggaggat tctgcggtct
     5221 gcaaggtgaa atccttgccg cagatcacga ctttgacttt atttaacata gcgttgtgtt
     5281 tcctttcatt caagtgcagt gctgcactgc cttttctatt atacaacatt tttcgcagct
     5341 tgaaaagagg gtattttgga aatttttcga aagttccgct ttattcggca ttctgacgcc
     5401 gttccagttc ctccaggatc agcttgttga ccttgttgac atcgccgcag cccagtgtga
     5461 ttaccaggtc tcccggctgt gcgtggttgc agatatactg tgtcacctgg tcgaagttct
     5521 catacttgcg ctcgtcagtg gtctccggat tgctttcgtc ctgcgggaac cagaccgcac
     5581 cggggatctt ttcacccagc tgccgtgtga agatgttgta ggtgttcttt tcccgggaac
     5641 ccataatgtc cgtcagtaca gcgtgatccg ggatggaaag cgcctctgcg aattcgtcca
     5701 gcagcatggc agtccgggag aacgtgaacg gttggaacac agcccagacc ttgcggaacg
     5761 gcatttccat tgccgctttc agcgttgcgg tcagttcggt ggggtggtgc gcatagtcgt
     5821 ccaccagagt gatgccgttt acttcgccct ttttctcgaa acgtctgcct gcgccacgga
     5881 attctgccag cccctttgcc acatcttcaa aggaaacgcc tacatacatt gccgccgccg
     5941 cagccgcagt tgcgttcagc acgttatgtt caccggcaac gtgcagcacg atctcgccaa
     6001 gcgatgcgcc gtggtgcatc agctggaagt gggtctccag accggaaacg tggcggatgt
     6061 cagccgggta gtagtcgcat tccggcgttt tgccgaaggt gatgcgttcc ttgccctcaa
     6121 tgccctccat cgctttgcag gtgttggcat cgctgccgtt gtagatgact gctttagagg
     6181 tgagctgtgc aaacttgtgg aacgacttga tgatgttttc taccgtgccg aagtagtcca
     6241 gatgatctgc gtcaatgttc agcagcacag cgatgtccgg atacagtttc aggaacgtat
     6301 ccacaaactc gcacgcctcg caggtcatga tgtcgctgga accgcttctg ccgctgccgt
     6361 gaatacaggg cagcttgccg ccgatgaatg cggacaggtc cacgccggca gtgaacaaga
     6421 tctgtgtcac catagaagtc gtggtcgtct tgccgtgtgt gccggcaacg cagatggcgt
     6481 tgtcatacca gctggtcacg atgcccagca gttcgctgcg ctccagcacc ggtacaccgc
     6541 ttgccttggc agcgatcagt tccgggttgt ctgccatgat ggctgcagtg tggacgatca
     6601 gatcagcgcc ctcgatattc tccgcacgct gtcccagata taccgggatg cccatgtccc
     6661 ggacagcctg gagcgtttct gtttcgttgt tgtccgaacc ggtgaggtaa tagcccttgg
     6721 tgtgcagaat ctgtgccagc ggatacatac cgctgccgcc gatgccgatg aagtggatgt
     6781 gcttttttcc ctttaagata tcaatgttca aaaaaatcac ctttttcttt cttgcaattt
     6841 tcaaatttat cgtgtatact tataggtaac gtacttcaaa ggagaagcgt ttgtgtttgc
     6901 acaggcgttt ctcctttttt tgttacgaaa ttgaaaattc gtttttcaat ttctgctggt
     6961 tgcccttccg gaaactgcat gaactctaag tcagcatggg gcagtttcca aaatgacaga
     7021 acggggcgca gcagatgacg gaggaggtca tcctatggaa atcacagata tcaagatcag
     7081 aagaattatc acagagggac ggctacgtgc agttgtttct gtgacggtgg acaatatgct
     7141 cgctgtgcat gacatcaagg tcgtgcaggg agacgagcgc ttgtttgttg caatgcccag
     7201 ccgcaaagat gagaacggcg tgttccgtga tattgtgcat cccatttctg ccgccgccag
     7261 aaaaatgatg gaatcgcaga tattggaggc atatcacgag catctggctc tgatggaggc
     7321 cgaagcccag gcagaggcag ctgcggaaac acagccggca gcagtctgaa tcgtctgcgt
     7381 caatcttaga tatggtccac aggtcgtcgg agtattcgct ccggcggctt ttttatgccc
     7441 gttttcggat gtgctgcgtt gtgacagtgc cttttgcgat cactcgcctt tgtgcagcag
     7501 cacccaggtt ttcagcaaag cgatctgcgt cttggcatcg ggaatctcgt tattcatgac
     7561 catctgcact gcctcttcca ggggcagctc tgtcacatcc agaaattcgc cctcgtccag
     7621 ctcctgttcc tccggcgcag acagttcctg tgccagatac atatggatga cttctgtatc
     7681 atatgccggc gtcgggaaga gttttcccag agacgtatat ttgccgcagg tgcgtcctgc
     7741 ctcttcccgg agttcccgtt tgccgcagtc tgccggatct tcgccttttt ccagcttgcc
     7801 ggcagggact tccagagtta cctgctgcat gggatagcgg aactgtttga ccatgagaac
     7861 ggtgcctttt tcggtgagcg gcacaacgca tacgccgccg gagtggtgta ccacatcacg
     7921 ctgcaccgcc ttgccgtcct ccagccgggc ggtgtctttg gtgacataga acaccttgcc
     7981 cagataggtg gtttcactgc cgattgtttt ttcctgtaag tgcattttac gatacctcca
     8041 tatcatcatc cgcagcagct gttttttcgt cgggttctgc tgcggcaggg gattcttctg
     8101 ttgtgtctgt ttcatctgtc agttccggca aagcttcatc ctcggcggag accggatagt
     8161 ctatacagca tacggtctct ttgttccggc ggatgcccag tttgcggcag atcagcagat
     8221 aaacgatcag caggatcacg ccgcccagtg tcagccatgc gcccagatgc agtccgggtg
     8281 tctcatagtg gaactcgatg gtgttgtctc cggcttctgc cggaacagcc atcaagccgt
     8341 tgtctacttc ctctacgtca accgtttttc cgttgacggt ggcagtccat ccctcatcgt
     8401 agggaacgct gaaaaatacc aggttcgggc tgtccaggga gatggtggca gtaaagccgc
     8461 tggaatcata ttggaaggag tcacaggcgg atgcggcacg gttgctgcac tcctggagat
     8521 actcctcttc cgtcatagaa acattgctgt ccgaaaccgg ctgcatccag ctgccgtatt
     8581 tctcgatctg ctcgctggac agcaccagcg cacgcatcag cagattggag cggtagctgg
     8641 tggaagtgct gttccacttg cccttggcga tatagttgga gaacgtgaat cccatgggaa
     8701 cataggcggt gttccggaac cggtaaaagc cattttcggt ggtgtcatag acaaagcccg
     8761 gcatactgca tttcggttcg tccgtggt
//
LOCUS       DAWENO010000079         8657 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3373_length_8657_cov_13.827831, whole genome shotgun sequence.
ACCESSION   DAWENO010000079 DAWENO010000000
VERSION     DAWENO010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3373_length_8657_cov_13.827831"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7732..7741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2085 a         2305 c         2017 g         2240 t
ORIGIN      
        1 gaatcgactt cacttcattg ccctgcaatg caattccagc ctcatagcgt tccagaatga
       61 aatactcatg ccgtgcctgc cggttgtcac aaattttttt ggttcccttt gtctgcactg
      121 cacgcacttc ctttctgtat ttcgtgttaa aatagtatag cacatttttc acgtcttgtc
      181 aagctttttt cagaatatct ttcatgtccg aatctcaact ttcgtgcaga aggcaagcga
      241 aaaagagcag cttttttatt ttttcgcatt ttccaaacca taaagcaaaa aacgactgga
      301 atttctccca gtcgttttct ttcagcattt gcggtcagtt cccttccctg ctgattttta
      361 ttctacattt ttcgtttcgt ctgccttata ttcaaaaaag tcgaagtcgg catagtgctg
      421 atgctttacc cggtctgcac aggtgattcc caccatagtc cctgtaaatt cgccgtactt
      481 acagtactca tcggagaact ttgtggtatc aaacggaatg ccgatcttct cgtagttctc
      541 gccgtcatag ctccaataga actgagaatg tctgccgttt atcactaagc ggaagtagat
      601 cggtacatca tccaccggaa tccgggtgtt cacgaattcc gtcttttcgc cgttctccag
      661 ctggatgatc gagatagcac tctgccccag cgtttcgctg tagtacttcc ggagattgat
      721 atagttcatg ttgtcgtagt aaaggatcag tcctgcgctg tgctgatgca cctccggaac
      781 aaattccatt tttgtggtga tctgtgcata gacactggtc agctttctgg caagaatgct
      841 ggtcttgttc agagaagttc ttgcctcctg tccacgcagt ctcacccagc ccggacgagc
      901 cgtaacatcc gcaaaccgct gcggcatgat tctcggcgca tagtaccagt tgcccagggt
      961 gtcgctgtcg aagtcgtcga aattcgggat ctgcggcaca ggatactccg gcagcctgct
     1021 ctcttcgcag tattcctttg caatgttcgt gccgtctgcc atgcgcagcc agccatcctc
     1081 tgtccacatc attttctgga tcgccgtttc tctgcccagt gtgcagcgca gctccggtgt
     1141 aaagggacgg gagcagagat gcaccagata tacctcaccc agggaagttt ccacatagct
     1201 gccgtggcca gccttttgca gcaccacatc cgggttgtaa tacttgggct tgaggtgatc
     1261 cggatcatgg cgctcgttgg aaaccgccgg attggatgtg acaatgggat tcttcgggtc
     1321 acccgcatag ggaccccaca cgttttcaga acgtcccatg gtcacgcagt ggttataacc
     1381 ggttccgccc tcggcgcaca tgatatagta gtaaccgttc cgcttggtca gatgcggtgc
     1441 ctcgatgcag cctctgtccg taccgccttt ccagatgcgt ttcggatagc cgatgatctc
     1501 tttcttctcc gggtcatatt ccaccatgca gatcgcaccg ggcttttcat agccggcacg
     1561 ggtttcccag tccagggaaa cgatgtactt tctgccgtca tcatcgtgga gaatggaggc
     1621 atcaaaaccg gaggaatgca gatataccgg ctcgctccac ggacctttga tgtccttggc
     1681 agtgataagg aagttgtcca cgtcaaagta tctggcattc atggagttca tcacgccata
     1741 gactacatag aactggtctt ccttttcgca gtaggtcaga cacggcgccc agatgccttt
     1801 ggcagagggc agctttttca agtcaacctt ttcgtcatcg gtcagcacgt gggtgtacag
     1861 ttcccagttc ttcatatcct tggaatggta gatgggaatg ccgggaaacc attcaaaagt
     1921 ggaaactgcc atgtaataat catcgccctt gcggcagatg cacggatccg gattaaaacc
     1981 cgggagaatc ggatttttaa gcattttcat ttcctccttc gtttgcgagt tcctttacga
     2041 tgccgtcata ttccttgtcc agcttgtaga acagcataac aacaacctgt gcgacacaga
     2101 ggattgccgg gatccagata aaggtgatct cagagcctgc gcttgcgcct gctgcgccga
     2161 tgacccagcc caggatagca gaacccagac cgtttcctac cttaaagcca aagctggatg
     2221 cagagttgat gagaccttca gtacgatagc cggttttcca ttcgccgtac tcgatggtgt
     2281 cggatacgat agcgaacata caggaagaga tgccggcgtt gccgatgccc tttaggacgc
     2341 aggcggtgat gatgccagcc atatttgtgc cgacaaagcc gaacattgca aagccgatgc
     2401 cataaacgac tgcgccgata atcagcatat tccgcttgcc gatcttcttg ataaggaatg
     2461 cagtgaagaa cataacgccg atctgcacca ggtttgcaac cagattcatg gtgccgacca
     2521 tattctcatt gtggagaatg ttctttgcgt agtaaacggc tgcgccgccg ttcagcgagt
     2581 agttgatgaa gatgaatacc agagtcacgg taatcatgat ccagtacttg ttcttgaaca
     2641 gcaggacaat gcctttcagg aacggcactt tctcctgctt ggtctccttt gccggtacaa
     2701 cacgttcttt tgttccggta aaggtaatca tgaatacgat cactgccagg atgccgaata
     2761 cagcgaatgt ctttgtccat gcggagaggt tatcgccgaa gaactgtacc agcggcaggg
     2821 tcagattggt gatgagcagt gtgccggtag ttgccaggat catacggaag atgctgagga
     2881 cagaacgctc gtactggttc tgtgtaatca gtgcattcag tgtggcataa ggcacattga
     2941 tcgccgtgta gaccacagag gacaccaggt tatacgtaat aaagatgtac accagttttg
     3001 aggtttcccc cagtccggac ggcacggaga acagcaggat tccggcaagg gcaaagggaa
     3061 ttgccatgcg cagaatccac ggacggcact tgccgaaccg ggacttggtt ctgtctacga
     3121 taacgcccat gataaggtcg gtgacgccgt cgaacactct cgacagcagc atgatggtac
     3181 cgatggtagc ggcactgacg tgaacataat cggtataata aaagagcagg aatgcggaca
     3241 ttgcggagta gatgatgtta cagcccagat ctccgcagcc ataggaaaaa cgttcccaaa
     3301 agctgatttt tgtttttgaa gcggaagcca tggtaggttt cctcctttcg tgtttttgtt
     3361 gtctctatta taaccgatat cccgacgact gaaaaggcgg tattcagaca acaaaatagg
     3421 gcaaatccga catttatgca gatttgccca tagatatcct attttaggaa tcgctgtacc
     3481 aagccagtct aagcccctgt tccgtgctgc tttatttccg aaactcccgg cgtttttcct
     3541 ttggcgtaca gtgatagcgc tccttgaaca gctgcatggc gtacttataa ttggtgcagc
     3601 cgtgatcttc aaaaatcgtt ccgatactgt gatccgtttc ctgcagatcc cggacaacct
     3661 tgtgcaggcg tatctcatac aggtactgca tcaccgtcag ccccagctgt ttcttgaaaa
     3721 gcttggacag atagtcggga ttgtagccga aatgcgctgc aatatcttcc atcacgatct
     3781 tttcgctgtg gtgatcctcg atataccgca tgatgttctt gaccttgttg atggaacgat
     3841 tccgcttatt gactgccttg tccgtcagcc ggatggaata ggaatgcacc agcgtataca
     3901 acagatcata gagcaggctg ttgaacttga gcagatacgc atccggacgc acatcataga
     3961 cgatgtacat atctgtgaaa attttcttga tgcgttccag ccgtgtgaca tctaccggat
     4021 gttcggggtg catatccacg tgaaattcca gcaggtcata tgccggaata tacttttcca
     4081 gcacagcgct ggggatcagc agcaccagcg ccttgttttt ctgcgccgtc accgaatgga
     4141 tcactctggg gttgacgatg gaaaattctc cggggtgcag cgtctggcgc acgttgtttt
     4201 caaactgtgt caccatgctc ccctcgatca tgtacaccag ttccaggctg ttgtgccagt
     4261 gcggcgcaac atagctgccg ctgtcctcgg acagataaaa ccgcacatca atatcggggt
     4321 tgctggacac gatctcatgc cgaatgctgt tattttccat actgcacctc atttctttcc
     4381 gggtatctct ttccatttga ttatagcata ttatctgcgc ttttgcaatg acagcagaaa
     4441 aaaagtattt tccacaaaat tcaaattata gaaaagtccc ccttgaaaag ggggatgtca
     4501 gcttaagctg acagggggat catcacaatc tatactcact tatggaatat ccacacaagg
     4561 cacgcctttt caaataaaat aacgataaca tttgttttgc aaggggtaat cctctgtcaa
     4621 acttgcattt ctcgccccaa tatgttaaac gattgatatc cccttttcag agaaagttca
     4681 gtctctgaaa aaacactttc caggagatca agacgtatga gactattttc caatctcttt
     4741 aagtcaaagg tcggcgctga aagcaagcag tctgaccttt gaaacaaaaa cagccccggc
     4801 agtaccgctt ttccacggta tgccggggct gttgtcattt ccggaaacga tcagtatttc
     4861 cagctgtttg tcttgacctg ggtgtcgatc atgtgcttgt aaatgccgtt ttccgcacgc
     4921 agcgcatccg ggctgcccat ttccgcaacc tttccgtcct tcagcacaac gatcttgtct
     4981 acgctgtcaa cggtgcgcat tctgtgtgca atgatgagaa cggtcttgtt ccggatcagc
     5041 ttggagatgg actcctgtat caaggactcg ttgtccacat ccagagatgc cgtcgcctca
     5101 tccatcaaaa tgatcggcgc atctttcaga aacgctctgg cgatggagat acgctgtctt
     5161 tcgccgccgg aaagctcaga gccgttttca ccgatcatgc tgttccactt gtccggcata
     5221 cgctctacaa agtcctcaca gtgcgccagc ttggcagccg ccatgacctc ttcgtctgtt
     5281 gcatcctttc tgccgatccg gatgttttcc agaacggtat tattgaacag cgtgacatcc
     5341 tggaacacga tggcatacat ggaaagcagt ttctccgggt cggtccggga aacgtccatg
     5401 ccgccgactg tgatctttcc cttgtcgttg tcccagaaac gagccgccag tctggaaacc
     5461 gtagtctttc cgccgccgga cggaccgatc agtgccgtta cctcgccctg ctttgccgta
     5521 aagctgacat ccgaaagcac ctgctctccg ctcttgtagg agaacgccac gtgatcgaaa
     5581 caaatgtcat atccctggtt ggtcagaacg tcgctgccgg tctgttcctc gtgggacaaa
     5641 atctcatcca tccgcttaca gttcacgtct gcactgatga ttgcagaaag gttttgcagg
     5701 gagatctgca gcggttcgta catccgggtc accaccagca ggaacataaa gaatgtgaga
     5761 acattgatct cgccgcttac cagaagcata ctgcctacaa ctgccacagt tcccatgccc
     5821 agtttcagga tcatctgtgc tgagcagaca aaagccgcat tggtgaattc tagaacggcg
     5881 gcgtgctttt ccactgcctt tatcttcttg gtcagccctt tcatgtacga gtcggtcatg
     5941 ttgtacgaac gcaaatcacg cacggtctcc agtccttcct ggataccgtc caggcagttc
     6001 accttgtaga ccgatgcctt ttccagcata tttttcagaa acttcttgga acaaagcacg
     6061 atcaaaaatg ccaccggcat gacccaaatc gccgccagcg ccatcttcca ctggaagaag
     6121 aacagggaaa tcacaatgat cgtggtggag atcatagcgc cgatcagttc cggaatccag
     6181 tgagaagacg ctgtctccag cagagcgcag tctgccataa tggtattggt cagatcggca
     6241 aggtctttct tgccaaagaa ggaaagcgga atcttccgca gcttttccgc aagggttctt
     6301 cgccgcacgc cgctctccac ataggtggaa aagaacgtgg aacgatactg aaaaatcgtt
     6361 gtcagaacga tcaaaagcag gatgaccacg attgccgcaa tgaaaaacgg cagcttttct
     6421 cttggcactt ctccatccag cagataggac gccagaaagt acagcagtcc caccggcatc
     6481 atcagcacca gatttgcaag tgcaacggca aaaattgctc tgatcatatc ctttgcgccc
     6541 ttgtccgaaa gcgcatattt atgctttaat ttttcaatca ttgcgcagca cctgtctttc
     6601 cgacctgcca gttgacagat ctgttgtatt cattccacat atgcgtatac acgccgtttg
     6661 cggcaagcag ttcttcatgt gttccgctct ccgcaatttt gccgtctgcc agcacataaa
     6721 tgcagtctgc attggtgatg gtcgaaagcc gatgggcgat catgatgacg gtcttgtctt
     6781 tggaaagctt ttcgaacgcc tgctgtacca gcatctcatt atccgggtct gcaaaagccg
     6841 ttgcctcatc cagaatcagc accggcgcat tcttcagaaa ggctcttgcg atggaaagcc
     6901 gctgtgcctc accgccggag acatagacgc cctttgcccc gatctgggta ttgaccccat
     6961 ccgggaactt ctcgatgata tccatgcact gtgcatcccg cagcgcctgc attacctctg
     7021 catcgctggc atccggtctg cccattctga cattttccag aatgctcatc ttgagcagct
     7081 tgctgtcctg gaatacataa gagacctggt tcatcagttc ctttccgtca atctccttta
     7141 catcagtgcc gccgatgcgg atagaaccgc ttttcacatc ccagaaccgt gcagccagtg
     7201 aggcgagggt agtctttccg ccgccggacg gtcccacaag ggcaatgtgc tgtcctgccg
     7261 gaatctccag ggtaatgccg tcgatcgcat tccgctcaga accgtcatag gcaaatacca
     7321 cattttccat ggaaacgctg gcatttgccg ggcttttcgg cttttctgcc tctgccagcg
     7381 gagaaagatc caggatgctg tccatacgat tcagcgcatc cgttacgatc atattggact
     7441 ccccggcata ggcaatcttc atcagcgtta cggtcagaat cgaactgatc agaatataga
     7501 acagcagatt cagaataaac gtatcggtca cgccgtgtcc ggtgaacagg tatgccgaaa
     7561 gaatcagaac ggcaaacaca gcgtttaccg cagtggtgaa tccgaccatg ggcatcatca
     7621 tgctcttggt ataggaaacc gtccactttt catactcgtc aatagccttt ttgaaccgac
     7681 ggaacgaaaa gatggtctgc ccgaaagtct tgaccaccgg aatgccacgc annnnnnnnn
     7741 ngccacgcac atactcgaca gcctcggacg acatggattc cagggcattc tggtattcct
     7801 tcatgtctgc cgccagcttt ggccccatca tcgccgaccc catcagcaca aaagcgatca
     7861 ctgccggaat cagacacata agccccagcc gccagtcaaa cactgccatt accgcaagca
     7921 gcgcaatggg cgttgcgatg gaaaccgcct tgtccgggag gttgtgcgcc agataagttt
     7981 ctgtggctgc gctggaatcc gaaacgattt tccggatctt acccgttccc tcgctttcca
     8041 cgtatcccag cggcagcttc ataatgtgtt ccatcatggc gatccgcata tttgcctgca
     8101 cccggaacgc cgccagatgg gaacacatca gcgccgcaat gtaaaacagc atggaaagca
     8161 gagcacctcc tactgcatac cagccgtatg tcttgatatg taccgcctgg ctgaaatccg
     8221 gccggacttc cagaacctct ttgataatgc gccagatctc ataaaacggg atcagagcga
     8281 tcactgcact cactgccgca agagccaatg aaaggtatgt cagatacttt cgctttccgg
     8341 cgtaccccat cagtctctga aacgaacttt ttcccttttg tttcaatgtg attcctcctt
     8401 tagaattata tagttagatt tcactaacct atatacaaac tttagagcgt gttctcataa
     8461 aataattgtg ccgaaaaaag tacaatttca tttttatgcg aacacactcc tgtaaacgca
     8521 gttccgcaaa aacaagcaca aattttacgt aagaccaagc actgctttcc agccggcata
     8581 gctgtacttg cggatatcgt ccataaaaag ctttgccttt tcgaagtcca tattgtggga
     8641 cacgatctca tagacat
//
LOCUS       DAWENO010000080         8613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3396_length_8613_cov_10.405936, whole genome shotgun sequence.
ACCESSION   DAWENO010000080 DAWENO010000000
VERSION     DAWENO010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3396_length_8613_cov_10.405936"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    261..270
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8018..8027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2219 a         2258 c         2193 g         1923 t
ORIGIN      
        1 ctataattta tggtgcgaca caactcaata cactttaagt gaaagtatac acttttatca
       61 atttcggtaa aagtgcatac tctattttcg tatgcttttt cttaaagtgt cgagttgtgt
      121 cgcaacaacc agagttatgc attttttcag tgcgtggctc ttggctgcgc acttttttta
      181 tatcattttt aggaggaaaa acaatgaaaa agaggattat gggtattgca ctggctgctg
      241 taatgggcgt ggtaagcatg nnnnnnnnnn cggatgcggt tctggctctg atagcaaaaa
      301 agacagcaca aaagttaaag tacagacaac cttaaccgac aacggacttt gggattacga
      361 gatgttggtt gatggtaagg tgttaaaagc cccaatcaca tcaaaaacat tgaacgccct
      421 gggatacgaa gtgtctgata atgataagag tgatggagat attgacacag aaggcgtcta
      481 catgtatgct atgaactgtg ttaacaaaga tactggggtc actataggaa ccagcgcatt
      541 gattaacaat ggcaataaaa ctatggacta tgccagctct tttggctcaa aagaccttga
      601 attctacaag ctgatgtttt ccggcggtga caatgaagaa aatgactata attttgaaat
      661 cacgaaaggt atcacaatcg gttctaccga agatgatgta aaaaacgcat ttggcgaacc
      721 agattatgtt acatattacg acgcatatat gtactttgaa aatcctaatg ctgtttcaga
      781 taagtctgag cctgattgca gagaatccgg atatgcgtcc tttaatttta gtgatggcaa
      841 agtttcggca attactttgt ctgcaaaaat gaatcgtgaa atttcgcagc aataaaccaa
      901 taaggattcc gaatcctaat cattcccatt ctttcaaccg aactatataa acaaaaaaac
      961 gctgtatgaa agtcaatcca ctttcataca gcgtttttat tttacaagtt gaaaagacta
     1021 tccttaccct tcccaggctc ctgcatactc cgacttcacg cccagatatt cctccagcgt
     1081 cagtccctgg gacatgatct cctttgcggt gtccacgccc acatagcgga tgtgccacgg
     1141 ctcgtactga tagccggtga tgttctcctt gccgtccggg aaacggataa taaagccata
     1201 gtctgcgcag tgcgccgcca gccactttgc ctcttcggtc tcgccgaaag catcatcaat
     1261 ggtgttgcag tcgatggtca gtccggtctg atgctccgaa tagcccggac gtgccgaaaa
     1321 ggtatctgcc atctcccatc cgtacagatc gttatagttg ttatagatct tcacctggta
     1381 cgcatagtcc cggaagtccg aaccgatgta cagattcagc ccttcctttt ctgcgtcagc
     1441 cttcatctgt tcaaaagcag cctttgtggt ggcatccaga cccggatcat agctttccgg
     1501 cagtccgtaa ctcttgttca cgatcaggat tccgtctaca taagtgcagc cgtctttctg
     1561 ctccaggtgg cgcactgtca ccggaacggt ctgggcgatg gcgtcattgc cctttaccga
     1621 aaccgtgata tcgcacttgc cggctttcac gccggtcaca ttgccggagt cgtccacggt
     1681 ggcaactgcc tcatcgctgc tgctccagac cagatcgccg gcagtcgctg caccggtggt
     1741 ggacatattc actgcaatgg cagccgtgcc gccttccagc acagccagat acgtcttgtc
     1801 cacgctcaga tccagaatgt tctcgttggt agtggctgcc tcagatgccg cactgctctg
     1861 tgcggatgtc ccctgcgccg atgccacaac gccgtttccg tccggaatga tgctcgtgcc
     1921 acgctcgtcc gaaaagaaca gcagccgcac cgcaaatccc agaataataa acatcacata
     1981 taatagtacg aggatgacac ccagccggga aaacttgtcc cagacgctgc ctttggattg
     2041 ttttgccata atgatccctt tctctttctc attcgctctt tgtttccaaa ttccctgttt
     2101 tttctccaaa ttgtaataat tctaacagga aaccactcta attcttacaa agcggttaca
     2161 tcccttgtat tataccacac ttttcctccc atgtcatttg atattttcac caaacatcac
     2221 acttgtgatt tgtgcatttt taagcacttt gtgcattatg tcacaaaatt gctctcgcaa
     2281 ccagatagaa tgcacaaaaa tttcagcaga tgtgatttca ttcttgaaaa atatcacaac
     2341 ccgtgctata ataagtaaga aatgaaacca agccgaagtc ggttcaaaag agccgcaact
     2401 acgcttattt ttggaaaggg gaaactccat gacgaaaaag aacaaattca tctccgttct
     2461 gaccgcagcg accctgctgt gtacggggat cggcactgcc ggcgtgacta cctcgctgcg
     2521 tgcagatgcc gcagagtcca ttgaatcctc tatggattgg gacacactga acattgccgg
     2581 cggcggcttt gtctccggca tcatcaccgg cgacgaccag atgtatgccc gcaccgacgt
     2641 aggcggcgca taccgctatg actacgagca gaaaaaatgg gtacagctgc tgggatttct
     2701 gaacgatgcc gacagaggct ttctgagcgt agatgcaatg tgcgttgatc cgaacgatga
     2761 cgacacgctg tatctgctgt gcggctgtgc atatttcagc gacgcacgga cagttatttt
     2821 ccgttcccac gacgccggcg agacctttga agagatcgat gtcaccgatt tgatccaggt
     2881 acacggcaac ggctacggca gacagaccgg tgaagcaatc gcagtcgatc cggacaatcc
     2941 ggacatcatc tactgcggcg gcgatgccac tgccggcgat tctgccctca ttatgagtga
     3001 ggacggcggc gacacctgga gtccggtcat gggctatgat aagctggggc tgttcgaata
     3061 cagcatcaag tggccgacat ggacagaaca tatggcaaga tctgtcgcag atgacgaata
     3121 cctgaacgtc aatggtattg ctacgattaa aattacagac ggcaaggtct acgtcggcac
     3181 ttccgtcaag ggcaaggcaa acctccacgt ggcagatgtg ggaagcgatg acttccagcc
     3241 gctgagcgaa gatctgccca ccgagcagat gccttccaga atcaacctgg atgcagacgg
     3301 caacctgctt attacctatg taaacggctt gatgttcgac cgtggcactg gctatgctta
     3361 caagtacagc cccaaaaccg gcgagctgaa ggatatcaca ccgacagaag gcatcagcag
     3421 caacacgaaa aaactgaatg tcggctacgg cgcagtgacc agcgacaaaa atgatgccaa
     3481 caaactggtg gcaacgacct gcgcacagtg gtactcccag tcctggacgg aagatgcctg
     3541 ggatcgggat gccattgcat ggggcgaccg tttcttcaag tctgaggacg gcggcgagac
     3601 ctggacggaa atgacaccgg gcaaccgtgc cagctggggc ggtccgctga ttgcaaacta
     3661 cctgcaggac ggcggtcaca gctggatccg ggacaaggcg atccactggt ccggctgcat
     3721 tgcactggat ccgagaaaca gcgaccagtt ctgggtcgtt tccggtaacg gtgtattcac
     3781 ctgtgaggac acctgggcag aatgccccac catccgtttt gccgcagacg gtattgaaga
     3841 agtggtatct ctggacttca tcagccgtcc gggcaaggat ccggtttcgg tcatcggtga
     3901 ctatgacggt ttctatcata atgcagacgg cacagcgacc cagctgacac ccagcatgaa
     3961 caagctgact gacaccactg catccaccgg cggcatcgca tactgcccgg caaatccgga
     4021 cgtaatggta cgtctgtcag agggcagcgc aaagggctac tacaccacag acggcactac
     4081 atggcaggaa ctgccgaaca ttccctgctc cggtgcaaag gctgccatca accagctgga
     4141 ggacggctct taccgcattc tggtcagcag cagcggcaag atctcctata ccgatgactt
     4201 cggcaagaca tggagcactg cttccaccag cgacagcctg agcagcacca tctggatgtg
     4261 tgtagacgaa aagaatcccc agtatgttta cgcatacggc tattactaca accagtatta
     4321 tttctacagc aagcccaagg ctgacatcac agacgcacgc tacattctga tggtcagcga
     4381 tgattacggc aagaccttta aggacgctca gaccatctgc cagtacgacc agtgcgacaa
     4441 cgcatatcgt atcgcttatc tggacgaagg cacatttgcc attgctgccg gctactacgg
     4501 cgcatatctg gtaaccgact acggcaaaac tgtcaccaag atggacaacg taagctactg
     4561 caagaccatg ggctacggcg ctgcacagaa ggaaggcgac ccgtacaccc tgtatatgta
     4621 cggcaagccg gcagactctg atccggaagg catctatcgc tctaccgact gcggcaagac
     4681 ctgggtactc atcaaccaga accacctgta cggcggtacc ggcaacggta actatgttgt
     4741 cggcgatatg aacaccttcg gcaccgttta tatgtccact gttggctgcg gcatcgtggt
     4801 aggcaagatc aacggcagcg aagatcccaa gccggtcact tcttccacca caacgacaaa
     4861 gcagaccacg acaacaacaa caaacaccac gactacaacg gcaaagacaa ccagtgacat
     4921 cgcaaccacc tctgcaaccg ataaggcgac ctcgaccgca gaaagctctg tttctgcaac
     4981 gggaacggtt tccacaacct ccgtctccgc tacaagcggc tctgtgatcg ccggttcgat
     5041 tctctacggc gacgtcaacc tggacggcag agtagatatc acagacgcag tcctgctgaa
     5101 caaggcagtt gccggcgcag tttccctgga cagcacagca cagaagaacg ctgactgcga
     5161 tgccaacaac gagaccggcg caaatgatgc gatccttctc ctgcagttcc tggttcacat
     5221 catcaactcg ctgccgtata ccgctgaatg attccgataa tctcatcaga gaacgtttct
     5281 cttctgaaca gatgcaaatg agctctgaaa catccttcta tacaaacacc tgcattggca
     5341 aaaccgccgg tgcaggtgtt ttgttttgcc cgaaactgca catactaagc gtgtgtgcgg
     5401 ttttcgcttt tcagaatgct gtccggcaaa aaaagcaagt gaaaaaagat ggcttttgta
     5461 ccgttttccc ataagcaaaa aagcatttac acaaaagaaa gaaaagagtt ttggaaaaat
     5521 tatagaaaag gaaatcactt tgtgatatac taggaatgtt agggcgctca cacgggggat
     5581 cgtgtgcggt gccgtgtggt acgcagggaa agctgcggac acaaaaactg tcgaaagacg
     5641 aggaggttct atatgagaaa agtacagttt acagaaaccg ttttgcgtga tgcaaaccag
     5701 tctcttatgg cgacccgtct gccttatgcg gactacgccg atattctgcc gaccatggat
     5761 aaggcagggt actattccat tgagtgctgg ggcggtgcga cctttgactc ctgtttgcgg
     5821 tatttgaacg aagatccgtg ggaacgtctg cggaaactcc ggaaaaacct gcccaacaac
     5881 aagctgcaga tgctgctgcg tggacagaat ctgttgggat ataagcacta tcatgatgac
     5941 gttgtggaaa agttcatcga gctttccatc aagaacggca tcgacatcat ccgcatcttt
     6001 gacgcactca acgacttccg caactgcgaa actgcgctga atgcaaccat gaagtatgcc
     6061 accaagaata ccatcgcctc cggctgtatc tgctacaccc agagtccggt gcataccgta
     6121 gaaaagtatg tggaaatgtg caaggaactc aagcgcatgg gattccagac catctgcctg
     6181 aaggatatgg caggcaccat gaccccgtat gaggctgaac acctcatcaa gggcatcaag
     6241 gacgctgtcg gcgatatgcc gctgatcctc catacccaca gcaccaccgg tatggcgtat
     6301 atgacactga ccaaggcgat cgagtccggc attgatgtca tcgacaccgc tacttcctgc
     6361 ttctccggcg gtacttctca gccgtccacc gagaccatgt tctatgcctg ccagcagtac
     6421 ggcatcgaga ccggtctgga tgaaaagctg ctcaacgaag tcaacgacta cttcaagccc
     6481 atcaagcaga agtatatcga caacggtcag atcaacccca agagcctggg aacagacgca
     6541 caggcgctgg tctacaaggt tccgggcggt atgctgtcca acatgattgc aaacctcacc
     6601 gatatgaagg caatggacaa gttcgacgct gctctggcag agatcccgca ggttcgtgct
     6661 gatatgggtt atccgccgct ggttacaccg ctgtctcaga tggttggcaa ccaagcagtt
     6721 accaacgttc tggttggcga gcgctatcgc cagatctcca aggagatcca gaactatatc
     6781 aagggtgaat acggcatcgc cccggctccg gtcagcgagg cgctccagaa gaaggttctg
     6841 ggcgaaggcg gcaagccggt agactgccgt attgaagacc agaagcgcac cggacaggaa
     6901 ttcgaagatg ccaagaaggc gctgggcgat ctggctcgtt cggaagagga catcatgagc
     6961 tatatctgct atccggatca gaccgtgaag ttcctggaaa accggaaggc acaggaggag
     7021 aacgaggccg tttataccat cgaggccatg taaggaggaa cagctatgcc actaaatgtg
     7081 ataagcgcat caagcatggg catcggcgag gcaggcatca ccgccatttt tggctactgt
     7141 gtcgttttcg tcggactggt cctgctgatg ctggtactgt atattatggg tgccgtgttc
     7201 aagtcaaagg caaagaaagc ggaggcactg aaagctgcgg aaaaggctgc cgctccggca
     7261 gaagcaccgg cagcagaacc ggaaaagccg cttgcaccgg gttctgccgg tcacatcaag
     7321 cgttttgatg taccggacaa ggaggctgca atgattatgg cgatcgttgc gaacaagctg
     7381 gatacacctt tgaatgaact gcacttcgta tcgatcaagg aggtcaaaga ggatgaagta
     7441 taaagttacg ctgaatgaca aagtatatga agtagaagtc gaggcaggaa aggctgtcct
     7501 gctggacgaa tacgaggctt gtgcgccggc tccggcggca gctcctgccg cagaggcagc
     7561 tccggcagcc acagcggctc ctgcaccggc tccggcggca aaggtggatc tgaaggccgg
     7621 cgaaccggta aattctccga tgccgggaaa cattctccgt gtcgaggtca aggagggcga
     7681 acagatcaag gccggacagc tgctggtcgt tctggaggcc atgaaaatgg aaaacgagat
     7741 cctggcaccg cacgacggca ctgttgcaca ggtcgttgtc tccaagggaa caactgtaga
     7801 gactggttct ccgctgatcg tcctggcgta aaggaggggt gtattcatga atatgcttga
     7861 aaccctccag aacctgctga atgactccgg ttttgtcagc tttttcgccg acggcggctg
     7921 gaagaacctg gtcatgatcg cagtctcctg cctgctgctg tacctgggca tcaagaaaca
     7981 gtttgagccg ctgctgctgg tgggcatcgc tttcggcnnn nnnnnnncat catggcgaac
     8041 ggcggactgc tggatatcct gtatatcggc gtaaagtccg gtctgtatcc gtctctgatc
     8101 ttcctgggca tcggtgcgat gaccgacttc ggtccgctgc tgtccagccc gaagaacctg
     8161 ctgctgggtg cagccgcaca gatgggtgta ttcctggcgt tcttcggcgc agtttgcctg
     8221 ggctttgacg gcaaccaggc agcgtccatc ggtatcatcg gcggcgcaga cggcccgacg
     8281 gcaatcttcc tgaccagtaa gctggcaccg gaactgctgg gaccgatcgc tattgcagca
     8341 tactcctata tggcgctgat cccgatgatc cagccgcctc tgatgaagct gctgaccacc
     8401 aagaaggaac gggaagtcaa gatggaaatg acccgccagg tctccaagac cgaaaagatc
     8461 attttcccga tcgtcgtttc tctggtcgtt atcctgctgc tgccgtccac cgctccgctg
     8521 atcggctgcc tgatgctggg caacttgtgg cgtgaatgcg gcgtgacaga gcgtctgtcc
     8581 gataccgtac agaatgcgct gatgaacatt gtc
//
LOCUS       DAWENO010000081         8589 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3405_length_8589_cov_8.638505, whole genome shotgun sequence.
ACCESSION   DAWENO010000081 DAWENO010000000
VERSION     DAWENO010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_8589_cov_8.638505"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3798..3807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2306 a         2011 c         2432 g         1830 t
ORIGIN      
        1 ctgccgggcg aaacttccgg cggcgtactc cgagggattc catccgcata tgcagacttc
       61 ctttgccacc accctgccgc tgggattcat cgggacaggg gaactgatgg atctggaact
      121 gtccgagccg ctgccctgtg agacagtcat ggaacgtctg aatgcggtgc tgcccgaagg
      181 catccggatc ctggaggcag gcaaagggca gcttgcattc aaaaaactgg cgtatgcccg
      241 gtataccgtc cgcattccct gtgaagatgc ggcggcgctg caccgttctt ttgatgcgtt
      301 ctgcgctcag ccggaaatta tcactgaaaa gcgtaccaaa aaaggcggca caaaagaagt
      361 cgatctgaaa cctatgctgg aggtgacggc ggtgacagaa cagccggatg ccttggaagt
      421 ggagctttgc ctgccggcag ggagcatcgc caatctgaac cccatgctcc tgctggatgc
      481 gtggaaaaaa cagagcggcg cagcgctgtc agccccggag atatcccgga cagaactgct
      541 cacagaagaa aaacaaaaat ttttttgaaa aaacacttgc attttctttg gaaatgtggt
      601 atgatataaa agcatttgaa atttgtgccg aatccggcag aaaatccgga ggtgcaaatg
      661 atccgcacgg tttccgtgtg aggttaatgc cggctgagcg cccgatggga attggcggct
      721 cagcagaacg acattaaatc ggacagtccg ctggcgctgc cgaacatacg gcagaagttc
      781 cggctctcat gcagctgaac agagagcaga ggaaacacgt gaatgaatgt tcgcaaattt
      841 taggaggata ttaaaatgga tgcattgaag acaatcagcg acgcaagcct gaagcaggaa
      901 gtaccggcat ttgcagtagg tgatactgta aaggtacacg taagaatcaa ggaaggcgac
      961 aagagccgta tccaggtatt cgaaggcacc gtaatcgcaa agaagcacgg cggtatcagc
     1021 gaaaccttta ctgttcgccg tgttgcacat ggctgcggta tcgagcgtgt attcccgctg
     1081 cactctccgg tcgttgaccg tgtagaggtt atccgtaagg gtaaggttcg cagagcaaag
     1141 ctgtactacc tgcgtgacag agttggtaag gctgcaaagg tcaaggaaca gattcgataa
     1201 aaaagaatca ctgcacaatg caaagacggg cagaatgggt gcgtatgtcg tcgtttgctt
     1261 catgccacct gccgtcggca gtaaaaaagg gacagtattg tccttttttt attatcggct
     1321 ggtcgctttt gcattgctgc gtacaattca ggaatggaga acgataaaat ggaacagcag
     1381 acaaaacagg aagaaatgca gcaggaacag aagaaaactt cctttctgag cgatatggca
     1441 gaagtgctgg aaacagtttt tgtttcaata tttgtcgtga tcctgctgtt cgcatatgtg
     1501 attcgtccgg taaccgttga gggcgattcc atgcgtgaga cgctgcacaa caaggatcgg
     1561 ctgctgatgt ccgacctgtt gtatacgccg aaatacggcg atatcgtgat cgtaaacaac
     1621 gagcattcgt attcctatga cgaaaacggc gacctggtag agggcagcgg actgccccag
     1681 gaaaagcgcc tcatcaaacg tgtgatcgct accggcggac aggagatcaa cattgacttc
     1741 gagaccggca cggttaccgt agacggcgaa gttctggacg aaccgtacat ccgtcagttg
     1801 acgctcacac gggaagacgg tttcgagtcc tatccagtca ctgtgccgga gggttactac
     1861 ttcgtcatgg gtgacaaccg gaacaactcc accgacagcc gttcgcagtt cgtgggattt
     1921 gttccccggg atgcgattct gggcaaggca gtgttccgga tctcgctgga cgatttcggc
     1981 agcatttata agaacatgaa ataagagcac aaaagaaagg cggcagaaat ggcagaagat
     2041 ctgatgcgga acatccaatg gtttccggga catatgacaa agacccggcg tatgatcgca
     2101 gcgaatctgt cgctggtcga tgccgtggta gagatcctgg atgcacgtat tccgcagagc
     2161 agccgcaatc cggaaatgga cggcatggtt ggcgcaaagc cacgtctgct cctgttgaac
     2221 aaggcagata tggcagaccc ggcggcaaca tcccgctgga tctccgctta ccgcaaacag
     2281 ggctttgtgg cgctgggcgt agactgccgt agcggaaagg gtatctctgg ttttgcaccg
     2341 gcggtacggg aacagctgct gccggaactt ttggaacggc gtgcggcaca cggcatgacc
     2401 ggcgcaccga tccggctgat ggtggtgggt atcccgaatg tgggaaagtc ctctttcatc
     2461 aaccggatgg cacagtccaa gcgtgcaaag gtggaagacc gccccggcgt gacccgtacc
     2521 aaacagtggg tgaagatcgg caacaacatg gaactgctgg atatgcccgg cgttctctgg
     2581 ccgaaattcg atgaccagag cgtggcacag aaactggcgt ttaccggtgc tatcaatgat
     2641 gatgtcatgg atctggaggc gctggcaagc ctgctactcc agaaactggc ggcgtcctat
     2701 cccaaggcac tggaagagcg ctaccgcatg acagaactgg acgagatctg tgaggacggc
     2761 tttgctatgc tggaactgct gggacggaaa cgtggtatgc tgatctccgg tggcgaagtc
     2821 aacacggagc gtgcagcaat tacattgttg gacgagttcc gcagcggtaa gctgggcagg
     2881 atcacgctgg aactgccgga acaggaggcg cagaatggct aaaactgcgc cgaaaaagac
     2941 gacagaggaa ctgtacgcct gggatgcgga aaagcggcag ggcggtttgc tgctctgcgg
     3001 tgtagacgag gcaggaagag gacctcttgc cggagacgtt tacgctgcgg cagtcatttt
     3061 agacccgtcc agaccgctgg tcgcaggctt gaatgacagc aagaagatga cggaaaagaa
     3121 acgggatgcg ctgttcgaag aagtgaaaag tgcctgcctg gcttactgcg tggcatcggc
     3181 atctgtcgaa gaaatcgaaa cgctgaatat cctgggagca accatgctcg ccatgcagcg
     3241 tgcagttgcc ggattgtcta tgcaacccgg actggtgctg atcgacggca accgtctgcc
     3301 gcagctttct gttccggcgg aaactgtggt gaaaggggac gcaacctctg cgtcgatcgc
     3361 cgcagcgtcg attctggcga aggtctcccg agaccggacg ctttatgaac tggacgagat
     3421 gtatcctcag tatggttttg caaagcacaa gggctacggg acaaaagcac actgtgaggc
     3481 gattttgcag tacggaccat gtccggcgca tcgccccagt tttttgcgaa agctatatca
     3541 gaatcatgaa aatgaaaaca actgagatcg gtcgcctggg agagacggag gtttgccgcc
     3601 gattggaaca gctgggctat tccatcgtaa agcgcaactt ctgcatccgt ggcggcgaga
     3661 tcgatattat tgcccagaat gcggaatatc tggcgttcgt ggaagtaaaa acacgaaagc
     3721 cgggaagtct gacttccggg tttgatgatg taacggtgaa aaaacaggct cgtttgatcc
     3781 aggcggcggc aatgtggnnn nnnnnnncac ggcccagctc tatctggcgg agcatcccac
     3841 gcagctccag cccaggttcg atatcgcctg cgtggaaatg caggggacac acgtgctttc
     3901 ggtggattat ctggaaaacg catttgatgc gacgggcagt gattttatat tttaggcaga
     3961 atacctgccg ggaaaagggt ggtaacagtg ttttacaaaa gcaccagaga cagcagcatt
     4021 cgtgtagaaa gcgctgcggc aattgcaaag ggaatttcgg aagaaggcgg cctgtttgtg
     4081 ccggagtcca ttccgtcgat ctccatggac gaactgaaat cgctggcagg tatgaactat
     4141 gcacagcgtg ctgcgttcgt attctccaag tatctgactg attatactga tgctgagctg
     4201 cgctactgca cagagagcgc atacacaaca aaggcatttg acaccgagaa cattgcagaa
     4261 atcgcacacc tgttcgaggg aacttatatg ctggaactgt ggcatggccc gacctgtgca
     4321 ttcaaggaca tggcactcca gatcctgccg tatttcctga ccacagctgt aaaaaagctg
     4381 aacatggaca aaaaggtagt gatcctggtt gcaacttccg gtgataccgg taaggctgcg
     4441 ctggaaggat tcaaggacgt tcccggcacg gagatcatgg tattctatcc ggtcgagggc
     4501 gtaagcgata tgcagaaacg ccagatggtg acacaggaag gcgaaaatgt aacggtatgc
     4561 gctgtaaagg gcaacttcga tgactgccag agcggcgtga agaaaatctt caccgaccac
     4621 accgtactgg atgccctgga aaaaggcggc atgacattct ccagtgcaaa ctccatcaac
     4681 tggggcagac tggttccgca gatcgtgtac tatgtttcct cttatgtttc cctggcagag
     4741 tccggcgaga tcgcttatgg cgacctgctg aacgtagttg ttccgactgg taacttcggc
     4801 aacatcctgg cagcatatta cgcaaagatg atgggcgtgc cgctcggcaa gctgatctgc
     4861 gcatccaaca ttaacaaggt gctgactgac ttcatccgca ccggcgttta taaccggaac
     4921 cgtcagttct atccgaccac ttctccgtct atggacattc tgatctccag caacctggag
     4981 cgtctgctgt atctgctgac cggcgaagat gatgcacaga tccgggaatg gttcacggct
     5041 ctggcagaaa agggaactta tgaggtaacg gatgctgtca aggcaaagct gaccgaacag
     5101 ttctacggcg gtttctgcga cgatgctgag acaaaggcaa ccatcgcaga actgtaccag
     5161 aagtacggct acacctgcga tacccacacg gcagttgctg taaaggtata tgaggactac
     5221 cgcaaggaga ccggcgatac cacaaagaca ctcatcgcat ctaccgcaag cccgttcaag
     5281 ttcagcgcaa gcgtgctgga tgcactgggc aaaaagccgg cagacggcac cgatgaatat
     5341 gatatggtcg agctgctgca cgaggtttct tcgatggaga ttccgcagtc tctggcggcg
     5401 ttaaagacca agcctcgtcg ttttgacggc agcattgata aatctgaaat gcagcagttc
     5461 gtattgcagg aactgcacct gtcctgaacg gggtgtgtat gcgtttattt accataggcg
     5521 acctgcacct ttcgtttggc gtaccggata agcccatgga tattttcggt ggctggaagg
     5581 gctatcagaa cctgctggaa caaaactggc gggaacggat cgccccggag gataccgttg
     5641 tactggcagg ggatttttcc tggggcatga ccttggcgca gtctgaggca gactttgcct
     5701 ttgtgcagaa actccccgga caaaagatcc tgctgaaagg aaaccatgat tactggtgga
     5761 cctcgaaaaa gaagatggaa gattttttcg tggcgcacgg atttgacacc atgcacattc
     5821 tccataacaa tcactatgct ttcgggaatt acggcatttg cggtacccgt ggctgggtga
     5881 gtatgcaggg agaggcggca gatgccaaga ttctggcgag agaagttcag cggctgcgtg
     5941 tttccattca atcggcagtg gatgccggtc tggaacctat cgtgttcctg cactatccgc
     6001 cgatctacgg cacatccatg aactatgaga ttctggaagt gctgcacgaa tacaagatcc
     6061 agcgctgcta ttacggacac gtccacggaa aagggcatca ccacgcattc cagggacgct
     6121 acgaagaaac tgaaatgcgc atgatttcta gtgattatct acaatttgta ccggaacaga
     6181 ttttctgagt tgtacaaaat ggagaactcc ggaaatgcac tagccaaaac cggagaaatg
     6241 tgctataatg aataagtgtg cataatactt tgcatgatat aaaattcctg tttccggata
     6301 atcctgccgg acgcagggaa ttgcaataaa tttattggag gaaaccaaag atgaatttga
     6361 aatcgtcaac aaagactgat gtaaacatga ccgaactggt cgttgaaatt gatgcagaaa
     6421 gctttgaaaa agcagttgaa gctgcttatc agcgccagaa gaagaacatc tctatgccgg
     6481 gattccgtaa gggcaaggta cctcggaaaa tgtgcgagag aacatacggc gaaaatgtat
     6541 tctatgagga tgcaatcaac gctctgctga acatggagct gccggctctg atcgtagaca
     6601 acgagctgaa cgttgtagat accccgaagg tagaagttac cgcagtcagc aaggaagacg
     6661 gcgtaaccat gaaggttatc tgcgttacca agccggagat ctctctggac ggctacaagg
     6721 gcattgaggc tccgaaggtt gtcaaggaag ttactgacga ggacgtagac cagcagatcg
     6781 agaatatgcg tcagcgcaat gcacgtgttg tttctgttga cgaccgtgca gctgagatcg
     6841 gcgatgaagt gaacctgaac tttgaaggtt tcttcggcga cgaggctttc gaaggcggca
     6901 agggcgaaaa ctaccagctg aaactgggca gcggtcagtt cattccgggc tttgaggatc
     6961 aggtagctgg tcacaagatc ggtgagaact tcgacgtaac tgtaaagttc ccggaagatt
     7021 atcagatgga agaatatgct ggcaaggaag ctgtattcca ctgcctcatc aacagcattt
     7081 ctcacgaaga actgccggaa ctggatgatg agttcgtaaa ggatacttct gatttcgata
     7141 ccgttgacga gctgaaggct gacgttcgca aaaagctgga agaaaacgct gaaaatgcag
     7201 cagcaaactc cttcgagaat gctgtaactc tcaagctgat cgaaaaggta accgatccga
     7261 ttccgcattg tatgtttgag catcgtgcag acagcctgat gaaccagttt gcaaaccagc
     7321 tgaaggcaca gggcgtttcc ctggatctgt atctccagta cactggcatg gatcaggatg
     7381 cactgaagga aacttatctg gaacgtgcag agaacgaagt aaagctgcgt ctggctctgg
     7441 aaaagatcac agaactggaa aagctggaag tttctgacga agacctggaa gcagaagttg
     7501 ctcgtctggc agaagagaac aacatgactc cggatgacgt gaaaaagcgc atctatgtgg
     7561 aagatctgcg tgcagatatg ctggcaacca aggctatgga tctggttaag gaatccgctg
     7621 ttgcaacaga cgctccggca gaagaagaaa ctgctgcagc aacagaagaa taagaaaacg
     7681 gcgaaagcca taaaaatgaa tgaaaaacag atgggcgaac tcttgggtcg ccctctgtag
     7741 tatgtgcgca tttttcgaaa tgtgtcgatt tggaaaggaa gatgaaacat gagtagttta
     7801 gtaccttacg ttgtagagca gaccagccgt ggcgagcgtt cttatgatat cttttcccgc
     7861 ctgctgaatg accgtatcat catgctgtcg gatcaggtaa atgatgccac cgccagccta
     7921 gtcgttgcac agctactgta cctggaggga caggacaacg aaaaagacat ttctctgtac
     7981 atcaacagcc ccggcggttc gatcaccgca ggtatggcga tctatgacac catgcagtac
     8041 atcaagtgcg atgtttccac catctgcatc ggcatggctg catccatggg tgcgttcctg
     8101 ctttcttccg gcgcaaaggg caagcgtatc gctctgccca acagtgagat catgattcac
     8161 cagccgctga tctccggcgg tctgtccggt cagtgcaccg atatcaagat cgcatcggat
     8221 cacctggttc gggttcgtca gaagatgaac gagatcctgg ctgaaaacac cggaaagccc
     8281 ctggaagtca tcgagagaga caccgagcgt gacaactata tgtatgctgc cgaggcaaag
     8341 gaatatggtc tgatcgacaa ggtgatcgca cacagataaa ggggagccga aatggacgaa
     8401 aaaagagaaa aacgatgcaa cttctgcgga aagccggagc atatggtaga aggtatgatc
     8461 tctgctgcgg atgtaaacat ttgcagcgag tgcgtgacct attgctacga aatgctctat
     8521 ggcccggtag acccggcggc atctgcgaag gagaaaaaga agaaatccaa agagccggca
     8581 gagatccat
//
LOCUS       DAWENO010000082         8563 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3426_length_8563_cov_7.426070, whole genome shotgun sequence.
ACCESSION   DAWENO010000082 DAWENO010000000
VERSION     DAWENO010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_8563_cov_7.426070"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    871..880
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1929..1938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4701..4710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6487..6496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1924 a         2289 c         2123 g         2187 t
ORIGIN      
        1 cggcaaccac gaagataatt tctatctgga agggctgtac atgaacgata ttttccggaa
       61 cgatttttcc gcatcttata ccattcacta tgcagacggc atgagttttg ccgtattcga
      121 tggtatgggc ggtgcagcat acggcgaggt cgcatctgag atcgccgtgc agacgctgcg
      181 gaagtatgag aaaaagctga aacacgccga cgggacacgt atgctggatc agctcatttc
      241 ttcgtatacc tctgaggcga acaatgccat ctgtgatatg ctcacagaaa agcactgcca
      301 gaacggcgga actactttct ccatgctcta ctttctcggt gatgcagtca agctgtatta
      361 cctgggtgac agccggatct accggtatca gggcagcgac ttaacgctgc tcacacggga
      421 tcataccgtt gccaaccaga aagtggaagc tgctatttat acagaagaag aagcaaagca
      481 cagccccgat cagcaccgtt tgacattgtt catcggttca gaccgcaaaa aggcagggtt
      541 aaatgcggac agcagacccc ctttgccgct ggaagtcggc acaaagtttg tactatgtac
      601 cgatggactg accaatatgt gtgcggacga ggaaattgca gaacttctct cccagaatta
      661 tttcaacgaa gcagcagtgc tggtgcaagc cgcactgcag aatggcggcg tggacaacat
      721 tacctgcatt gttttggagg tactccccgc cacccaggac gcagaagacg actagtctcg
      781 ttttctctcc ggtatatctg ccatagaaaa acggcacgtg gtatttttcc acgtgccgtt
      841 ttgtgtttta ctcgttactc tcagatgcgt nnnnnnnnnn attctttttg gtgcggcttg
      901 cagagtgcat ccccttatag tcaatatcat cttctgtggt acgcgcgatc aagtccatgg
      961 tgcggacaac ttctaccggg aaagcaccgg cagcagtttc accagacagc atgattgcac
     1021 tggtgccgtc atagatggca ttggcaacgt ctgtgatctc cgcacgggtc ggacgaggat
     1081 tggtaatcat ggactccagc atctgtgttg cggtgatgac ctgcttgcca gcctgatagc
     1141 ccttccggat cagctgtttc tgcacggacg gaatctgttc aaacggaatc tctacgccca
     1201 gatcgccacg ggcaaccatg atgccgtccg atgcctccag gatctcgtca atattccgga
     1261 cgccctctac attctcgatt ttcgcaatga tgcgaacatc aaaccagccc agactctggg
     1321 tgtacttccg caggtagtca atatccgcag aggtacggac aaagctggct gcaatgaaat
     1381 caaagcccat ttttgcagca aattccaggt cgttcttatc actttcgctg agataaggca
     1441 tagacagctg tacgcccggc acgttgatgc ccttgttatt ggaaaggaca ccgccgtgga
     1501 taacgtggca gacgatagtg gttgcattta ctttttcgac aaccatttca atgacgccgt
     1561 cgtttaccag aatgtgtacg cccggtttta cgtcacgggg cagattcttg aaagagatgc
     1621 tgccgacctt ttcgtcacac agcacatcat ctgttgtcaa tgtataggta tcgcctgttt
     1681 cgatggtgac cggcttgtca tccacaaagc gtcccagacg aatctccggt cctctggtgt
     1741 ccatcagcgt tgcaatggga agtcccagtt ctttccgcag gcgtgcgatc tgttcaaacc
     1801 gcttttcatg cgtcgcatgg tctccgtggg aaaagttaaa tcgtgccaca ttcatgccgg
     1861 aaagcatgat ctgccgcatg acagaatcat catctgtcgc aggtccgatc gtacaaacaa
     1921 tcttcgtcnn nnnnnnnnct cctccggcgg caggaacggc aggctgctgc tggtatcaaa
     1981 ccgcagccgc tcatccggct ggagaatggc atttgcctct ttccactgtc cccatccgcc
     2041 gaagtgggct gcaatcagcc gcagtttcgg gaactgtcgc agcaccattg ccagccggta
     2101 cgggtgcgaa aaatctgagc gtgcatcgcc catgtgcatc aggatgggca gcccggaact
     2161 ctggatcata tcatacatgg ggtatgctct ggggtcgtca atgggaaagt tctgaaagtc
     2221 cgggtgcagc ttgacgccgc agagatgcag ctgctgcagc tgttcaaagt cttccgccac
     2281 atggtcggcg taagggtgca atgtgccgaa tgcggtacac atgggatagg ctgcggcgca
     2341 ggacgccaga aagctgttga tagaatgcac ctgtgtcacc gtggtcgccg gcgagcagat
     2401 cagaaagtga tctacaccgg cagccatgcc gttttccagc atctccgcca ctctgccgtg
     2461 atactccata ggcaggtcat aaaaatggga aatgccctct gttgcttttt cggcgatctt
     2521 gtcggggaaa acgtgggcgt gtgcatctgt gatacgataa ccgtttttca aaatgattcc
     2581 tcccctgttc agagcagctt tttgattttc ggatgacttg ccatgagccg tttggttccc
     2641 acctggtcga aaccgacttc cagcatagca tcatttgcca ttttcgtgat agccagaaca
     2701 gtgccgtccc caaaggttgc atggcgcacc cgttctccca catggaattc tttcggctct
     2761 gccttgtatt tctgtggttt cgccgatgcc atctgccgct gcagcgaaat agactgcacg
     2821 gattccggct tgtctgcgga aattccacgg cgagactgcc gctgagaagc ctcctgttcg
     2881 atcagctgcc ggtcgatctc tgtcaagaac tgggacggca gattgcgcat ggtcataccg
     2941 aacagcattc ggctcgcagc ggtagtcagc gtcagctttt tcttggcacg ggtaagtgcc
     3001 acatacgcca ggcggcgttc ctcttccaga tcttcccggg aatccaggct gcggtttcca
     3061 gggaaaacgc cctgctccat tcccaccaga aagacgttct gaaattccag acctttcgca
     3121 gaatgaacag tcatcagcat gaccacatcc tgctctggct cgtacttgtc cacatcggta
     3181 tacagagaaa tctcctccag gaatccgccc agggacggct cctccgcctc gttctgatac
     3241 gtcagaatgg aggacttcag ttcctctacg ttttccaggc ggaaagtgcc ctcttcgccc
     3301 atctgttcca gcgccgcacg atagccggtt ttctccagga ccacatccag gaatttttcc
     3361 agcggcagtt cccgttctgc ctcgatcagt tcatcccaca tcacagcaaa ctttttcagc
     3421 gcattcatct ttttggaaag taccggatac tgatcggcgt tccgcattac ttccagcgga
     3481 gaaaggtgca gatcctgggt gatgtcctgg agcaacgaca aggtggcgtc tccgatgcca
     3541 cgtttcggct cattgacgat tcgttcaaaa cgcacccggt cattgggatt ttccacaatg
     3601 ctcatatagg cgatgatatc ccggatttcc ttgcggtcgt agaaacgagt gccgccgtag
     3661 atacggtacg gcacgccgct gcgcaccagc gcacgctcca gcatattgga ctgggcgttc
     3721 atccggtaca gaatcgcatg gtcgccgtat ttcgcaccct gtttcacgtt ctcaaggatt
     3781 ttcttggaga catatgccga ttcgtcggac tcgtccgcag ccttatacca ggtgatcttc
     3841 tcccctgcgc cggcatccgt ccagaggtgt ttggactttc tgccgtcgtt gttggcaatc
     3901 acgctgttgg cggcatccag aatgttctgg gtggaacgat agttctgttc cagacgaatt
     3961 acggtacact gcggaaattc ctcttcgaat cccagaatgt tctcgatcgt cgcaccacgg
     4021 aaacggtaga tgctctggtc atcatcacct actacacaga ggttgccgct ggcatgggtc
     4081 agcagcgaga tcagctggta ctgtgcgtgg ttggtatcct ggtactcgtc caccagaacg
     4141 taagggaaac ggtactgata ctttgccagt tcctccggga atcgccggaa cagttctacc
     4201 gtcagataaa tgatatcgtc aaaatccagt gcattggagg cacgcagatg ctgctggtat
     4261 acaccgtaca actttgcaat catcatcttt cgatagtcac cgccggcatc ttccagcatc
     4321 tctgccggag agatcatggc atcctttgcc cggctgatct cctggatcac actgcggggc
     4381 gggaactgtt tttccgacac attcacgtca gaaaggcagt tcttcatcag ccgccgtgca
     4441 tcatctgcat cgtaaatggc aaagtcgctg tcatacccca gcagggtgat gctccggcgc
     4501 aggatgcgca cgcacgccga gtgaaaagta gacgcattga cagatgcggc gatctcctcc
     4561 cccagcgtac ttgccaatct ggcacgcatt tctcccgccg ctttattggt aaaagtgatc
     4621 gcaagaatat tccacgggcg aatcggtgca atcgccagaa tctcacgcag gcgttccaca
     4681 tctgtctccg cttttccgtc nnnnnnnnnn gcatcctctt ccggcaaaaa gccgtctgcc
     4741 ttggcatagc cgtctccgaa atagatcatg ttgacaatgc ggctgatgat tgccgttgtt
     4801 ttgccgctcc ctgcgccggc aagcaccagc accggaccgt ttacggagaa aacggctttc
     4861 tgctgctgtt cgttcaaaga actgaagtat gtttgaaatg cctttcgttt cagcgcaatg
     4921 cactgctgtt ctgtcatgtt tttctctccc tgctttctca atattgtctc agatgtctag
     4981 catctgtctg gttctatcaa ggtatatgtc cgaatgtggg acacgctttt attataccat
     5041 attccgctgc gtttgaaaag atgcactgca aacttttcac aattttccgc tgccgcatcc
     5101 tttttcaaga aaacatcatg ctgatatttt cttgtttttt cgcatgatgt tttctatttt
     5161 atatcccatg ttttatcaat tgacaattgc agaaaaattg gatatgatat acctgtccgc
     5221 tgctgccggt tctaatgtgc tgttctccga cgtatcatag aaacaggaag tctaacaacg
     5281 cagcggctct gccgcatcgc acaggaggtt tttatgcagc gaaaagcgat ctcatttttg
     5341 attatgtgta ccatagccac cgcatttttc accaatagtt ccgtatccgc cgccgctgag
     5401 gaattcgtac cgggcggcag tgctgcactg cctgccgtct cttcttacga atcctggatg
     5461 cagtttgaaa cgccggatac caacacccct gcaatcgaca cagacggctg tctgaactat
     5521 acactcgcca agctgagcgc acggtacaat ctgccagttc ccggtgtcag cctgctctcc
     5581 gattcctatg attattacac cgctttttcg gaaaaggtac tgtccggcgg cgcaacgaaa
     5641 atggacagcc tggcaaatca gtaccaggcg tatctcaccc ggcaggaatc cgtctggctt
     5701 tccggcaatc tggaagagcg catcgcacag gcatatgcgg tctgcgctga aacggggaca
     5761 gaacgctcac agagctacat cctgcaaatg acaacagcat ccggcagcga tcactatgta
     5821 ttggtagatt acatcgacac cgaacagaaa cggctttacc tgctggattc cggctcgtgg
     5881 tatgtggagt atctgggaga tgccaaaacc atggaaaagg gctactgcat taccgcagtc
     5941 cacccgtttc atattttcaa aatgccgggt gatacagacg gcgactttct gctgacgcag
     6001 tgtgacattg ataatctgct gcacggcgca tacgaggata cgctttccgg agacgcaaat
     6061 cacagcggca cggctgacct ttcggatgcc gtctatctgg cgcatctgat acagtctgtg
     6121 ggacatctga gtgttcagca cgctctggaa acagagatac ctccggtcgc cactgcaacc
     6181 acatctgccg atgcgctgcc cactttctgg aacagtcaga tttttcagcg gcagcttgcc
     6241 aaatgagaaa gagtccgcct gcacagttac cgtgcggacg gactcttttc tattacatct
     6301 taggaatcct ggttgtttac ctttacaaac tgcatgatcc agtcagagat ctgtacattt
     6361 gcctccttct gatatttctc ctgcaattca ttatagcgtt cttcctctaa gccagcctca
     6421 gcggtcagct gccagcccgg ctgctgcgat gcgccgacat aataaattag gcaggaaata
     6481 taggaannnn nnnnnnaact gtccaggatc gtcacatccc ctgcttttct ggattcgtca
     6541 aagcaccagt cacgccaggt ggtcatcagc tgcgaccggg tcacattctg gtacagtcca
     6601 ccggacacgg aggtgtcttc ggtatactgg tcagccagtg ccgcaaaagt tgtctcttcc
     6661 ttgtcactgg cgttccattc ctccagcgcc ttttctgctg catcatgggc gcccgatgcg
     6721 gagtcgtatg tgtttgccgt gaaaaggatc tgccgcaggt tcaccggatg ggtctcgtca
     6781 cgctccggtg cagcagtcag caggcaaaca gtaatcgttt tgttgtctgt atcggtgaaa
     6841 agcttactgt ccccgacttt ggtgtccgca gaaaacgccc attcgctgat ggagtcattc
     6901 tgggtatagg agacatcttc catgcagagc gtcttgacct gggatgccag ttcctcttct
     6961 gtcagcgaag tgttctgcgc atagtaatca tgcacccact gttcaaagga ttctttggtg
     7021 tcacagcgcc gcagatttcg tgccagttct tctgcctggc tctggctgat ggtggtgctg
     7081 tcgctggaat cgtcatagga aaatgaaaat tccatatagc tgcacacatc catttctgta
     7141 ggatttttct cgtaataggc gttcagttcc tcttccgtga agttgaaatt gtcctggata
     7201 tgcgtgctga aactctgcgc cttgaagtac agttccagtg cgtgtgttac gttttcctct
     7261 tttaccgtat ctggcatggt gctgaaatca atctgttttt tcgcagacgc caccagagaa
     7321 gagatgtctt cctccggcgt gtaattttcc gcatctgccg cctcacagaa tgccagaatg
     7381 ttcttggcat actcttccgc agcatcggtg aaatatgcct cccaggtcgt atcattatcg
     7441 taattctggt cggctaacgg tttcgaggtg tccggcacat tggaagagcc ggactcggaa
     7501 ttgctggtga tgtagccgtt atagatctgc cacgcataaa aagagacctc tgccgcattc
     7561 agcttatagt gcgccgtctt tccggcagaa agcagctggg tcatcggctt ttcccggacg
     7621 aaccaggtcg ttccgccggc aacgactgcc acagccgctg cgcagcacac aattgccgtg
     7681 acagtctttt tggtgcggtg tttttttcgt tttgcctttt cggattcctt tcgctttgct
     7741 ttacgctgtt ccgccaggaa cgcctgtttt tccgatggtt tctttttatt tgccatattg
     7801 tatcctcgta tttctattgc acaaatcatt ctcagcgtat atcgcctttg tacaatgcag
     7861 tttcttttaa cagtatagca cattttcaga caaagtgcaa gaaaaaagcg aaattttgtg
     7921 tccttcccca ttttccgtgt tattccgccg ggatggaaca gattaaatcg ctgcacagcg
     7981 ggcatactca aagggacagg aggcgtatta tggcttttca aaaagtaatg atttccggta
     8041 tcaacacatc cgaactcacc gttttgaaag aggaagaaaa aatcgccctg ctccgggaga
     8101 tacgggacac cgggagcaaa gccgcacggg acaaactgat ccaggggaat ctgcgtctgg
     8161 tactcagcgt tatacagcgg ttcaccggga gaggtgagga tatcgacgac ctgttccaga
     8221 tcggtgttgt gggtctgata aaagcgatca acaacttcga tttgaccaaa gaagtacggt
     8281 tttccaccta ctgcgtcccc atgataagcg gcgagctgcg ccggtatctc cgggacttca
     8341 accagatcaa ggtgagccgg tcactccggg atatggcata ccaggcaatg caggcaaagg
     8401 aacggctgac agcaaagctg caaaaagaac cgaccgtcag cgagattgcc gaggaaatcg
     8461 gcgcaaaacg ggaagatgtc gtggtggcgc tggagagcat cagcgacccg gtctcaatct
     8521 acgagccagt atattcggaa tccggggatg cgctgtatgt cct
//
LOCUS       DAWENO010000083         8541 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3437_length_8541_cov_12.592977, whole genome shotgun sequence.
ACCESSION   DAWENO010000083 DAWENO010000000
VERSION     DAWENO010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_8541_cov_12.592977"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2250 a         2198 c         2288 g         1805 t
ORIGIN      
        1 tcagtcggta gagcatgcgg ctgttaaccg cagggtcgtt ggttcgagtc caactggagg
       61 agccagaaaa tgaaagcgaa tcacgaaagt ggttcgcttt ttctgtttta tatgccaaag
      121 gaggtgcggg aatgctgcga attgcaattg tggatgatga aacggcagaa attcaaaaat
      181 tgcaggagct ggtggaacaa ttcttccggg aaaagcaagt ggcgtatcaa atcacaagtt
      241 atatcagcgg agagactttc ttggtggcgc agcagcagtg cgatttgatt ttcctggatg
      301 tgcagatgac aggaattgac ggcattgcca cagcacgaaa tattcggcaa aacgaccgaa
      361 aggctgcgct gatctatgtc agcaactttt cagagaaaat ggcagattcc tttgcggtgc
      421 atccttttgc ttttctggaa aagccggtca tgcgggaaaa gctgtggatg cacttgcagg
      481 attacctgga ttattacgag ccccagccgg tctgccagaa cgatgcgctt acgctgaaag
      541 gcgagcatgg cagcctgatc gtccgcgtgc aggacatcct ttatctggaa tatgcgggga
      601 accgcaaggt gcagatacac ctgaaacagg acgaaaaaac ggtttacggc agcattacaa
      661 aatttctgca gctgctggag ccgtttcact tcatatcacc acacaaaagc tatatcataa
      721 accccagaca gattcagtcg gtctgccagc tggacattat tttgaccaac caggacagaa
      781 tccccattgc gcaaaagcgc cagaaacaaa tcgtggcgca aatcagcgac tacctgcatc
      841 agcacttaaa ggagaatctc cgatgaacct gtttatggac gtctttttga acatctgtac
      901 gctcctctcc ggctatctgg cgctgtggaa actgctggac aagcgtgaaa tgcccaaccg
      961 ctttctgtgg aactgcgcca agtggggcta tgtgctgctg gcggcatttt tgcaggcgtt
     1021 ggtgagccag ctgcatatcc cgtggctgaa cctggtcacc attgcgattt cgttcgtagg
     1081 catgacaaag ctgttcttcc gctgtaacct tgtgccactt ttgctgtacg acacggcggt
     1141 aatggtctgc ctgtttgcgg cagatatggt ctccagcctg gcggtctctg tgtcgctggg
     1201 ggattccgtc tcggaaacgc tgcaacgaac agacgtgctg ctggcccgct atttgctgtg
     1261 catcattttg tgttttatgc tttgcagcat ctttcccctg tttttcaagc gccgggaact
     1321 gtacgagatc tactggtatg aggtgattgc ctacgggctg ctggcggcgt tcgagatcac
     1381 ttcggcggca tatattgtgc atcgcctgca attcttttcc tcggggctgt tcgtgattct
     1441 cttcacgatg ggctgcctgg tgctggacat ctatctggta ttcgtgtttt accagctggc
     1501 ggagagccgg aaacgggaaa aggaatactc gctgattcaa cagcagtcca acatccagct
     1561 ttccgtatac cgggagctgt cacagaaata cgccgactcc gtccgaattg cccacgatgc
     1621 caagaaacac gtccgtgcgc tggaagatct tgtgcagagc gaacaggcga gccagtaccg
     1681 ggactccatc tgtgcggagc tgaacaagct ttacccggcg ttcgaacacg agaatccgct
     1741 gttttccgtc ctcatcaacc atgcgctcta tcgttcggag cgcaacggca tccggctgga
     1801 tctgcggctc catgatgcag acttgtccat gctttcggac atggatctga ccatcctctt
     1861 cgccaacctg ctggacaacg ccattgaggc ttgcacatca ctgccggcgg aaaagcgaaa
     1921 gatccagctc atcctggaac aggaaatggg ctttctcatc atccatatca ccaacccctt
     1981 tgcggagctt gcaccgctgc cgcaccataa gtatggctct accaagcccg gtcatatggg
     2041 cgtgggactt tcgaatgtgc ggcagacggt ggaaaagtat cacggcgttt tctcggtgga
     2101 tacggatgct gaggtgtttg ccgttgcgat taccattccg gtggcacaaa aatgaaagaa
     2161 ctatttccca aaggtgagga aaattgcagg aaaccagcgt ttgatgcaga taaaaagcgt
     2221 ttggtggaaa tgccttgcag aatgctggga aacctgctat actgaaacca tagggaaaat
     2281 atgaaaaagt gaaaaacgtt ttgcggcagt gatgccgtaa taaatttcgg gcagcaccgt
     2341 atctccttgt acggcactgc ccgatttttt tctatgatgc aatcaacgaa aaaagacttg
     2401 actgcaattt acagtcaagt cttttccaat ttggcagggg caccaagaat cgaactcggg
     2461 acacgtggtt ttggagacca ctgctctacc agctgagcta tacccctatg ataagatgtt
     2521 ttatacaggg aacctgcaac ataaattatt atagcacaaa aaaatacgct tgtcaaccgg
     2581 tttttgcaat tttttttatt tgacatttgc acaagatgta cacatatctg aaaatgtacg
     2641 gaattccggc acaaacgggg cagaaaaggg aaaaactgcc ccacggtttc gacaaaagct
     2701 gacaccggaa tctgctataa tagacctgtc ggagatcgaa ggacaccttg tgccgcagcg
     2761 tctccggcag gtctgacaaa aggaggcttt atgatgcagg atctttggaa acaggtagca
     2821 gaacagtatc atgtctcggc agaggaagta caggcggaga tcaccaaggc gatcgaaacc
     2881 ggaatgcaga cggcggaaca ttctgtgcgc cgggcgtggg cggagattcc acggcgctgt
     2941 gatgtgccgg aaccggaaga ggttgtggca tggcttgtca tacaggcgct ggcggcaggc
     3001 gtgctgccgg acggtgccgc agaacggcag gaatatgccg taaaacggtg acacagcaca
     3061 cagcgccccg gaaaagaaac ccggcgatga tacatcttct gacaatccct gcaaaaagga
     3121 cggcaaagcg ccgtcctttt tgcgtttcag acagccgctt tttctggaag gattttttta
     3181 ccggttcaca ataaatccaa ataaaccgcc ggaaaatccc ttgcatcttc cgggaaactg
     3241 tgctatacta aatagtatac agtatcgccg ccggatagaa cggcggagac ggaaaaaagc
     3301 attccggata ctgacattga agagaaagaa gtgcaaaata caatgacaag aattacaata
     3361 ttctccggct ttctgggcgc cggcaaaacc actttgatcc aaaagctgat ccgtgagggc
     3421 tacgtaggcg aaaagctggt gctgatcgaa aacgaattcg gcgagatcgg cattgatggc
     3481 ggctttttgc aggatgccgg cgtagagatc accgagatga actccggctg tatctgctgc
     3541 agcctggtgg gcgatttcgg caaggcactg gaaaaggtgc tggaacagta tcacccggac
     3601 cgcatcctga tcgaaccgtc cggcgtcggc aagctgagcg acgttatccg tgccgtgcag
     3661 aacatccacg cccatgacgt agagctggac ggctttacca cagtggtaga tgccaagaag
     3721 tgcaagatgt atcagaagaa cttcggagaa tttttcaata accagatcac atatgccagc
     3781 tgcctgattc tgagccacac cgcaggtctg tctcaggata agctggatga ctgcgtgcgc
     3841 cgtctgcgta cctgcaacga aaaagcgcct attgtcacca cagactggga tcagctcacc
     3901 ggcaagcagc tggtagaggc aatgacccag aagaacaccc tggatgacga actccaggaa
     3961 ctgctggctg aggcggcaga gcatgaccac caccatcatc acgatcatga tgaccacgag
     4021 catgaacatg agcatgacga gaactgcacc tgcggctgcc atgaccacga tcacgaccac
     4081 gagcatgaac accatcatca cgaccacgag gaacacgacg agaactgcac ctgcggctgt
     4141 cacgatcatg accacgatca cgagcatcac catcaccacc atgcagatga agtgttcacc
     4201 agctggggcg tagagtctgc acagaagtat acccaggagg agatcaccaa tgcgctgaag
     4261 gcactggaag atgcagacaa gtacggcatg atcctgcgtg caaagggcat tgtcccggca
     4321 gaggacggca catggatcca cttcgactat gtaccgggaa ccccggatgt ccgcaccggc
     4381 agcgccggca cgaccggacg gctttgcgtt atcggctccg gcattcatga ggatgccctg
     4441 gcagccctgt tccacgcaga ataaaaggag gacaacaaca tggcaggagg acaacagccc
     4501 caggaaaacg acgttccggt atacctgttt accggctttc tggaggcagg caagaccaag
     4561 tttattcagg agaccttaga ggacaagcgg ttcaattccg gcgaaaagac catgctgctg
     4621 ctctgtgaag agggcgaaga ggaatacgac atttccgcaa tgccctgcca ggatatctac
     4681 atccgtacca tcgaagagcc ggaagagctg aacccggcgc acctggagga actgctgaaa
     4741 gaatgcggcg caaccagagt ggtgctggaa tacaatggta tgtggcagct ccagcagctg
     4801 ttccagaatc tcccggacga ctgggcggta taccaggaaa tgttctttgc ggatgcgacc
     4861 acattcctac agtataacgc caatatgcgt agtctggtag tggacaagct gaacacctgt
     4921 gaactggtgg tgttcaaccg tttcaccaag gacatggaca agatggaatt tcataagctg
     4981 gtgcgtggcg tcagccgccg gacggacatc gcctatgaat acacagacaa ccacgtagaa
     5041 tacgacgaga tcgaagatcc gctgccgttc gatgttaacg caccggtgat ccgcctggca
     5101 gacacagact tcgctctgtg gtaccgggac attatggaag acccgaagaa gtatgacggc
     5161 aagacggttt cgttccgtgg catcgtggca gtagacccga cattcccgcc gaataccttt
     5221 gcggtgggca gacacgtgat gacctgctgc gtggaggata tcacttacag ctgcgttgtg
     5281 gcagagtggg aaaaggcaaa tatgctccag acacggcagt gggtgcaggt aacgggtaag
     5341 atccatgtgc agagacataa gctgtaccgt ggaaaaggcc cggtgctgca agtgcaggaa
     5401 gtcgtgatga ccagcgcacc agagcaggaa gtggcaacgt tctattaagg cgaaacatac
     5461 acatggtgga aaactgcgcc ggacaataaa gtttttcgat ccagcagttt ttcaaaaatg
     5521 ctggcgagga ctagggcgag aagccctggt cgctgtcctc gataaagtag ggggcgatgc
     5581 ccagcccgtt gcccagcgga aatggcaacg gcagacttgc ccaaggatag caagccacat
     5641 cgccccggtg tcttacgagg aaaacgggtt cggtatagca aagctgcgtt caaaaagaaa
     5701 tatcttggaa aagtcccagc gggatttttc caacgtatgt ttccacccat tccgcacaaa
     5761 ctccctacag ctgcaaatgc agccaaggga ggcgaatgca gatgaaaaat cgttggacag
     5821 ccggcttgtt gtccggtctg ctgacagtgg cgctgctccc ggtgactccg gtcagcgccg
     5881 tcagcggcga agtgatcggc tatggacagg gaaccgccgt ggacagtgaa aaccgtcctc
     5941 aggatgccgt ggcattcgac gcacagtatg cgcagtacgg cgcctatgct accacacccg
     6001 accacagccg catccttctg acctttgacc agggctatga aaacggctat accggacaga
     6061 tcctggacac gctgaaagaa aaacaggtga gcgccgtctt tttcctcacc ggcgactacg
     6121 ccaaaaagga aactgacctt gtgaaccgca tgattgcgga gggacacgtc ctgggcaacc
     6181 acggcatgac ccacgccagc ctgcccacgc tgaccgaatc cgaggcaaag gcagagattc
     6241 tggatctgca ccagtatgtg ctggacaatt acggctatga aatgcagtat ttccggtgtc
     6301 cctgcgggga atactccgag gcggcgctgg agacggcaca ggaatgcggc taccgcacct
     6361 tgttctggag cgatgcctat gtggactgga aaactgacgc acagcccgaa ccggcacagt
     6421 cgctggaacg gctgaaggca cacgcccacg gcggcgagat cctcttgctg cactcggtgt
     6481 cttcgaccaa tgcggcgatc ctgggcgacc tgatcgacgc acttcgtgca gagggctaca
     6541 ccctgtagcg attcgcagaa aatgaccgaa caacccggct tttgtccggg ttgtttttgc
     6601 acaggacacg ggaacttgta catatcctac aacaatgcta tgatttcaca ggggaagcta
     6661 tgtgcaatac atcgaaaatt caaaaagcat agaaaaccta ttgacaccat aggtgaaatg
     6721 tgatataata caaccatagc aacgaaaccg aaccgaaaaa tgccggaaac ggcagcggac
     6781 atgatagaaa ggaatatgga ttatggcaaa tcaggaatat cgttttgaaa ccaagcaggt
     6841 acacgtggga caggaacagc cggacccgac cactgacgca agagcagtgc cgatctatgc
     6901 gaccacctct tatgtgttca aggattctgc acaggcagca ggccgttttg gtctgaccga
     6961 accgggtaac atctacaccc gtctgatgaa cccgaccaac gacgtatttg aaaagcgtat
     7021 cgcagcactg gaaaacggcg cagcagcact ggcaactgca actggctccg cagccatcga
     7081 ctactccatc cgcaatatcg caggcagcgg cgaccacatc gtttcttccc agaccgtata
     7141 cggcggcaca tacaacctgt ttgcaaacac ctacgcagag atcggcattg agaccacttt
     7201 cgtagatgca cacgatccgg caaacatcga ggctgccatc cgtccgaaca ccaaggcagt
     7261 ttacgttgag tctctgggca acccgaactc tgacgtggtt gacatggagg caatcgcaga
     7321 gatcgcacac aagcacggtc tgccgctgat cgtggacaac accttcgcaa ctccgttcct
     7381 gttccgtccg ctggaacacg gcgcagatat cgtggtacac tccgcaacca agtttatcgg
     7441 cggtcacggt actgtaatgg gcggcgttgt tgtagacggc ggtaagttcg actgggcagc
     7501 aagcgacaag ttcccgggac tgtccaagcc gaatccgtcc tatcacggcg ttgttttcac
     7561 tgaggcttgc ggcaatatcg cttatatcat gaagctccgc accacactga tgcgtgatac
     7621 cggtgcgaca ctgtccccgt tccattcgtt cctgctgctc cagggtctgg agaccctgtc
     7681 cctgcgtgta gaacgtcacg tggagaatgc gctgaaggtg gtagaatatc tgaagaacca
     7741 tccgatggtc aagaaggtaa accatccgtc ccaggcaacc ggcaaggcgc tggaactgta
     7801 caacaagtac tatccgaacg gcggcgcatc catcttcacc tttgaactga acggcaccaa
     7861 ggagcaggca cagaagttca ccgaatctct gaacatcttc tctctgctgg caaacgtagc
     7921 agacgtaaag tccctggtga tccacccggc atctaccaca cactcacaga tgacagagga
     7981 agaactgctg gcagccggca tcacaccgac caccgttcgt ctgtccatcg gtatcgagca
     8041 tattgacgat atcctggcag acctggaaca gggttttaag gcagtgcagt aagagaaata
     8101 ccgatagcga tttcgtaaga gagaactggt tatcaataag aaaagcgggg agcgccggaa
     8161 accagcggca gtccccgctt ttttgttccg ttttctctat gagaaaaagt tttatgaaat
     8221 agtctcctct tctagaggag atgcccgata gggcagagga gtctgtcgat tttctcaccc
     8281 ttttctccgt gcgccaaaag catggcgcag agatttcgct gtctgcggac agcgaccagg
     8341 gcttctcgcc ctggacctcg ccagcatttt tgaaaaactg ctggagcgaa aaactttatt
     8401 ttctttgaac aacgaaaaat gttgaaaatc ttattgcacg caaagcaatt ttttaggaaa
     8461 gtcttctgtg aaaaaagagc aagtcagtgt atactgatag gggattaacc cctcagtcaa
     8521 gcctacggct tgccagctcc c
//
LOCUS       DAWENO010000084         8411 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3494_length_8411_cov_10.999521, whole genome shotgun sequence.
ACCESSION   DAWENO010000084 DAWENO010000000
VERSION     DAWENO010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3494_length_8411_cov_10.999521"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    954..963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6598..6607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2315 a         1798 c         2091 g         2187 t
ORIGIN      
        1 tgccgctgca aacgtcgagc agagtatcac gaatatcaga agtatcacgt tcatagtttt
       61 cttgcgtttt aggtcttttt tcagtatcct cagaaacata gtctttccct caattcttgt
      121 cattggcttt ttctgtacta tgattataac ataagaattc ttaacaagtc tttaactact
      181 gggaagatga cagataatgt gactctgttt ctcaaacttg atgcttttta gcgtacattc
      241 cgaacgttgc aatttgagtg gtgtagattt tttcatattt ttttgataca atactgttat
      301 tcttctgaaa ctctgtagag ctgaaaattg gaaggcatca taaaacgaga agggattgag
      361 atggagcaaa tgaaccgtaa taatccggtt gaactgacaa atatgtgcat gatctatgac
      421 gatcagggca atgtgcttgt tgaagaaaaa gttggtaagg actataaggg catcgtcttt
      481 cctggcgggc atatcgaaag cggcgaaccc attgttgatt ctgttattcg ggaaatttat
      541 gaagaaacag gattgactgt atccaatctt gaactttgcg gtgtaaagga ttgggtcaaa
      601 gaggatggta acaggtatat cgtgttttta tatagaacga atacatattc cgggacaatt
      661 caatcatcat ccgaaggcaa tatcttttgg atgtcattag aagaacttca agcaagagaa
      721 ccattctggc acatggacaa aatgattgaa attttttgcc ataaaaacta tagtgagttg
      781 tttttagatg gcagccatgg aaatagagta ccgattttga aataaaagca gggacaatca
      841 cgggggaaac tatcgcaatt actgatgcgg aaagagaaag caggcacttt actaatataa
      901 agtacctgct ttttgtatag ccgttttctg tctgttctaa aaggcgttct tgtnnnnnnn
      961 nnntatgata attagagatt tatgatgcgt aaaataagtg gaattacaga aattattctc
     1021 gaaaaacaat ctgcaaaagg agaaaaaatg cgaaaaagaa tcctatccct taccttgagt
     1081 tttgccgttt tgctttcatc tatgtccatg tcagcggtaa gcgtatctgc ggcaaacgtg
     1141 gaagaaacag caggaacgca gctgcaaacg attctgatgg acaacgatgg aaaaaatctg
     1201 tgggtaacag aaagctgggg cggcgcaaac attatcccga atacgatctg gacaacatct
     1261 gatctgtacg actattttta caacggcagt ctgtcgtttg aggtgcagag cagcggacag
     1321 acaagttttc cgtttcgtat cggcatcacg tctcattctc acaatgaaga tgtgaccgtc
     1381 aactggacag acctggaaaa atatcagaac gccgtaaccg catctccgga atggacaagc
     1441 tataccttgc ctttgtatga tctggcagag atcatctcgg agaaagggtt cgacaagcac
     1501 aacgtgtgga aaatcagcgt tggcgctgtc ccgtccgggg aatcggcatc gttccgcaat
     1561 atgaaaatat cttcggatga tgaggaaagg cagtatccct tcattaaggt aaaccaggtg
     1621 ggatatacct gtgagggcga gaaaaatgcc aaggtttcct gctttgcaaa attcggttct
     1681 ctgagcggca agaggtatga ggtcgtaaac aaggattctg gaaaagtcgc ctattcgaat
     1741 atgctttctg atgctgtcgc agatgaaacg atttcggggg agtccgtcta cgagatcaat
     1801 tttgatgccg taatagatga gggtacttat tttatccgga taccaaatgc agatctgaac
     1861 acatctgcgc tgaccccaag agataaggaa gaagatctga aaacggatac catcgtttca
     1921 gttccttttg agatcgggga tgatgtgtat gatgaaatgc tgtctgatat gagcaaatac
     1981 tactactatc agcggcaggg aattgatctg gaagaaaagt atgccgggga gttcgcaaga
     2041 aagaatctgc accccaatga tgtctctgtc agaagatggt cagacaggga caaccccaat
     2101 gccgaaacat ttgacgtttc acagggatgg tacgatgccg gagattacgg gaaatatgtt
     2161 tctcctgcag caacctcggt cgaaaatctc ctgcttgcct atgaactttt cccgcaggaa
     2221 ttccgaaagc tcgatcccaa tattcccgaa acggataaga caagtgatcg gtattccgat
     2281 tctcctgcga ttctcagcga gatcaaatgg gaactggata tgctgctcaa gctggaacat
     2341 ccggacaaag acggctcgtt ctatgttgct gccaattaca aggacggcac gatctacatt
     2401 gaagatacct tgtacagcac ttcggattac acttccggcg cccaggaaac tgacctgcgc
     2461 tctcaccttg caacagcgga tatggcggca atgcttgccc atgcgtatct cgtctataaa
     2521 gatattctgg cgtatgccga ctttgcagaa cagtgcctgg aaactgctgt ccgtgcctgg
     2581 aactgggtca cagattcttc caacgaaaaa catatgtcca ttggtgcggc aaatcgtacc
     2641 tatacattca cacaggagga actggacaga agtatgtatt gggcagccgg tgctttgtat
     2701 cgtgcctcca aaactgccgg aaacaatgca gatgtgtatg aaaactatct gattgccaat
     2761 tgtgaaaacg aaaacgtgaa cacctgcttt accggaagtt ccctcggata cagccataag
     2821 ggaagagcat tcctgggatt tttccactat ctgtatcaga atgatgcgcc gtcagacaag
     2881 ataaaaaccg ttttcagtaa gtttgagccg tggagaaagc gcatcctgaa ttatgacagc
     2941 tttggaacag attatcccga ctggggctac tggtggggtt ccaacatggt tcttgcacag
     3001 agttccatga cgctgctgct cggtagtatg gttacggagg gaagtgacag tattcccgat
     3061 gcggtgctgc attccaacca gagtgccttc aattatattc tgggcgtaaa tccggtatct
     3121 ttcagttacg tttcgggata cggcgagaac agcgtaaaga atatctatag tgcaatctac
     3181 accaaggatg caaaactcac gccgtacaaa tgcccggatg gatacgtgac agaaggcgca
     3241 aattccacaa acaacagaca tctttcgaag tataacggca agtgctacat ggacagcgat
     3301 gccgagtgga cgaccaacga aaacacgatc tatggcaatg cagcgtttat tctgctgacc
     3361 gccgctgtta tgtcacaaac ggagactgac ccagtgcagg gcgatgttaa cgcagacggg
     3421 aaattcgaca tcgcagatat tgtcaccctg caaaaatggc tcctcgctgt ccccggtgtg
     3481 cagttggcgg actggaaagc cgcagacctg tacacagacg ggaaagtgga tgtgttcgac
     3541 ctttgcatga tgcggcagaa gttgttcccg gcagagaata cagccaatca gattcacgta
     3601 aaaaatacag aggaactgaa aacggctctg gaaaatgcaa aggcagggga tgagatcatc
     3661 cttgccgaag gtgaatatgt ttacagcggc gaaacgccaa agggatatat gttcaccggt
     3721 tcagcggacg gaacaaaaga aaaccccatt gtcattcgct ctgaaaaccc ggaaaaaccg
     3781 gcgattctct ccggttcttc caccgctgaa aattacgttt tgtctgtttc cggcgactgg
     3841 tgggagatca gagacctaaa ggtgacaaat gcccagaaag gtattatgct ggacaattcc
     3901 aatcacacaa caatcagcaa ttgcaaagtg tacaacatcg gttctgaagg catccacctt
     3961 cgggacaaca gctccagctg cttgattgaa gattgcagcg ttcatgatac cggcgttgtg
     4021 tctccgggat acggcgaggc gatttatgta ggcagcgctc aaagcactac agggtacggc
     4081 tatgaatgcg acaacaatac catcagaaac tgtaagctgg gaccgaatgt cgcagccgaa
     4141 cacgttgaca tcaaagagta caccaccgga acaacagtag agaactgcac atttgacgga
     4201 accggaatga gcggtgaaaa ttacgcaaaa agttttatca acatcaaggg caatgactgc
     4261 gtgatccgaa acaatattgg ttatcggaac ggctgtacag ccatccagcg tgcattcgaa
     4321 cagaacaacg tggcagacgg ctggggacaa aacgctatgg tatacagcaa caaggtctat
     4381 atggatacag caaccaatgc gctgggcaag aaaatgtatt tcttgaatgc ctgggattgt
     4441 tccgccactg tctgggacaa cttcatggcg tatgacggcg aacttttctc cgttgacaac
     4501 gaggacgacc agtgggatta ttataactgc aatctgctga cctacggaaa taagtgatgc
     4561 tgttcctttg gtatccggga ttcacattca cccttgacaa ccaccctgca actatgctac
     4621 aataaaaagg caaaaattga aaaaagggag cagaatccat ggaacagtgg gatatttacg
     4681 atgaaaacaa gcagaaaacc ggtcgcacta tgaaccggaa cgactggcat atgaaaccag
     4741 acgagtttca cctgaccgta ctgggtgtat tgcagcgtcc ggacggcaag tatctgatta
     4801 cacgccggaa actggacaaa gagtgggcgc ccggctggtg ggaagttccc ggcggcggtg
     4861 tccgtgccgg agaggacgga gccgatgcag tgcgccgtga gatcctggag gaaaccggaa
     4921 tcgacgtcac cggcgcaaaa ggcggctatg cgttctccta taagcgtgtc aatcccgagg
     4981 aacagaacaa ctacttcgtg gacatttaca agtatgtgct ggacttcacc gaggcggatg
     5041 taaaggtgca gccggacgaa gtgacagagt tcgcactggc gactgcggag gagattcgtg
     5101 ccattgccgc acagggcatt ttcctgcact atgacagcat caaagaggca tttgtctgag
     5161 atcattttac aataacacaa acgcccctgc ttttcacggt ttgtaccgga aaggcagggg
     5221 cgttttgtta tgttgtcata gacttaggaa gaatagcttt tcagtatcac gtcttttccc
     5281 agcagatact gcgtcagcag gacaatatct gctatgctga atacgccgtt gccgttggtg
     5341 tcctgtgggc tggcagtcag cacatcatct gtgctctgcc atttggtgat gttctgccgc
     5401 atcagtgtca gatccacacc gttgaccagc ttgtcactgt tcacatcacc tgcaattatt
     5461 ttgctgtccg gttctgttgt attattgttt ccgccgccgt aatagtcgga aagggaaatg
     5521 ccgttggaac ccagtgcaat ggtatgatcc aggctgataa acttaccgtt gtcatcctgc
     5581 cacagcgccg gcttgaccag ggcgtatttg tcctcgtccc acttgccgaa gttgtcgtag
     5641 accagaccgc cggtgtcgcc ggagttttcg ttaaagcacc agaatgtgtg gtggatgcgg
     5701 ttctcgatca tgaagtcccg gagatatgtc atccactgtt cgttcttgtc gccgtccatg
     5761 aatccgcccc attcgcccat gagcagggga gcgacgccct cgtcctggag gaagaaccag
     5821 ttgtcatacc agcagtcttt cagcagagtt tcctgggtga atccgtcgta gaaccatttc
     5881 tgttcccata ccagcggacc gtagtcgtga ggggagtaga ccagctgact ctgatatttg
     5941 cccaaatcaa tgggatattt ctttgcgccc cggaagtttc cgccccacca tgtgccgtag
     6001 tagtactctg tcatggtggt gtagtcgatt ctcggtgcag tccagtcgta gccctctttg
     6061 gggtaaacct ctgtaccctc gaccataatc agcagttccg gattcttttc cagtactcgt
     6121 gccgcacagg tctctgcggc atatttccag ttggtcgggt cagtggagtc atcccatttc
     6181 gccatctgat cctttacgtc agctgtaccg tgcggttcgt ttttcaagtc gatggcaagg
     6241 atggtgtcat catccttgta ataatctgca aaccatgcca gcgcatcgca ccagtcctcg
     6301 gtgctgaatt tgtccgtgta ccagaggctg acctggtgac cggctgaggc ggtctctgcg
     6361 ctgtgaatgt cgatcatgat cttgatgccc agctcccggc accatttgac cgccatgttc
     6421 cagatatcaa agctgtactt gatcgtacct ccctcgatgc cctcttcggt cagttccggg
     6481 ttggtgtact ggttgacctt tggcgttgcc gggtcgggat caccgttttt ccattggagc
     6541 aaaatctctg tggacatggg aatccggaga aagttgaacc cgtggtcggc gatctgcnnn
     6601 nnnnnnngca tatcgtgcat attctgcgac cagaccccgt cgaatacctg gctgccgaca
     6661 ttgtagccga accagttgca gccggtgatc cagaccggat tgccgtcctt gtctacgatc
     6721 tcggcgttgt cgttgacgtg cagccagtcg tcgtgatagc tgtccggctg tgtggcagcc
     6781 tgtgccgcca gagataccgc cggggctgat gtgccggcaa gcatagttcc tgccagagca
     6841 gccggcagca gcactttttg aaaaatgtga tgcagcataa agtcctccct gtatcatgtg
     6901 cgtgcggaat ccttccgcat ctgatttccg ttttgcctat attgtatcac aatctgtggg
     6961 aaatgtcaat tgtttttgtg ggaattgaga gtgcaaagct gcattgaaaa aaatatgata
     7021 tgcttaattt ttgaaattca tagaatgttc aataaaagtt gtttattttt tttttttttt
     7081 tttgcggtat aataaacact atcatgatgt atcatggaaa gttgtgcttt tgcgcaatga
     7141 caattttatg aagggagtct attggaatga aaaaaatgaa gaagtttttg gcaggcttgg
     7201 ctgccggggt gatatgtgcg gcggcactgc cgctggcagg ggcagaactg ccgaatggaa
     7261 atggcttggt gttggcaaaa gagatgagtt ttcgttatgg agtttcggaa ggagaatata
     7321 cggtttcaac agaaacaggc tcagttgcac tgaattatgt tgtttggaat gatggtacta
     7381 tatccattaa tgattgccct gagtctatta caggtacatt ggaaatcccg tcggaaatcg
     7441 aaggaaggcc ggtaactgga atttatagtg ctttttttga ttgtgtttcc ttaactgaag
     7501 tcataattcc agacagcgta acaagcattg gaagttcagc ctttgaaaat tgcactgctt
     7561 tgacagatat ttcgattccg gatagtgtaa catatattgg agattcagcc tttgaaaatt
     7621 gcactgcttt gacagatatt tcgattccgg atggcgttac agaaattgga tattcagcgt
     7681 ttgagaattg cactgcatta gcggaaatag caattccgga cagcatcgaa aatattggtt
     7741 atcacgcttt tgagggaact gtatggatga aagctaaact agcggaaagt ccattagtga
     7801 ttgcaagtca tattttaatt gatggaacaa cttgttccgg cagtgttatg attccggacg
     7861 atgtaacgga aattgaattc aaagcatttg aaaattgcac tgccttaacg gaaattacaa
     7921 ttccgggcag tgtaaagaat ataggctcag atgccttttt ggaatgttca aacctagaaa
     7981 aagttaattt atcagaagga ttgtcgtaca ttggagagga ggcttttcga gactgcactg
     8041 ccttaaaaga aatcattttt ccggaaagca cagaagaaat cagctataat tctttccgag
     8101 gatgcacaaa cctagagacg gttgtgattc cagagaatgt tgcaacaatc gaaggctcag
     8161 ctttctggga aacaccttgg attgcaaaaa tgcagaagga aaatccgctt gtgattatca
     8221 atggaatctt agttgacggc agaacttgta caggtaaggt gattattcca gacacagtga
     8281 caaaaattgc aagttgggct ttttgtggat gtggaacaat gcaggaggtt caaattccgg
     8341 agggtgtaac tgaattattg gaatcaaatt tttatgattg cagttctttg gaaaaaataa
     8401 caattccaat c
//
LOCUS       DAWENO010000085         8367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3513_length_8367_cov_12.344803, whole genome shotgun sequence.
ACCESSION   DAWENO010000085 DAWENO010000000
VERSION     DAWENO010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_8367_cov_12.344803"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1903 a         2201 c         2143 g         2120 t
ORIGIN      
        1 gcacggttga cccggctgat ggtggcggtg cttgcgccgg tctcttcgac gatctcatga
       61 aatacgtgac cctcgtgcag catttttgcc acctgtaacc gctgcgccat ggcgttcagc
      121 tcctggtagg tgcagagatc cttgaaaaag gcgtaacatt cttccggaga acgcagcgtc
      181 aaaatcgcct caaacagcgc ctcctggtct gtattctgtc gttctttctt catgtatctt
      241 ctctcttcct tcctcatatt ttctgtgaac acactccaat gatattgtac catatcagtt
      301 ggataatgta aatactgcac aggaaatttg tttggatttc ggcaaaacca ccagaaaacc
      361 ggctgtgttt ttgacgaatc ggaaatggga ccggctctgc tttgcaggat ctgttgcctt
      421 tttcggcaga tatgatggaa acgggcagcc aatttggaaa aacataccgg aataccgatg
      481 cttttccggt ttcggaacat tcgcacagaa tatgcaaaaa aatcaaaccg cacctgtgag
      541 attcccattc acagatgcgg tttgactgca tttttatgat ccaatcagga gttgttttga
      601 agaaacaggc gctcagacat cctgcaaagc gtcatatccg gtctcgccgg tacggatctt
      661 tacaacatct tctacatcgt aaacgaagat cttgccgtcg ccgattctgc cggtgtacag
      721 gaccttcttg actgcgtcga tgatcttttg cggcgggatc tttgccacaa cagcctcgac
      781 cttgaccttc ttgaggagtt tcgttccctc ggtcttgacg ccacggtagt aggactcgtt
      841 gcccttctgt gagccgtagc ccattacgtg tgtcacggta acgccgttga cacccatcga
      901 ggacagggtt gcctggagtg cgtccagtct gtccgggttg cacataacga ccagcttggt
      961 catctttgcc tttgcgccgt ttgccggttt cataatcatt tccgattccg gaatggtcac
     1021 gccggttgct gctgcgtcag cggcatctga tgccatagaa acggtagacg gcatgaagtc
     1081 tgcataagcg gaaggcaggt tgtgttcggt tgcgtccaga cccagaactt cttcttctgc
     1141 ggaaacacgc agaccgatgg tctttttgat gatgaagaag gtgagggcga tcatgacgga
     1201 agtccatgcc aggatgcaga caacacccag tgcctggaca cccaggacag tgaaaccgcc
     1261 gccgtagaac agaccgttgt atgtgtcgtt gtccggaacg gaagttgttg caaacagacc
     1321 aactgccagc gaaccccaca gaccgttcag gcagtgtact gcaacagcac ctaccgggtc
     1381 atctacgtgg agaacgtgat ccaggagcca tacgccgaag cataccagca gaccggcaac
     1441 aataccgatg attgcagaac cgaaagcatc gactacgtca cagccggcgg tgattgcaac
     1501 cagaccagcc agggatgcgt tcaggcacat ggagacatct ggcttgccgt acttgatcca
     1561 ggtgaagatc atgcaggaaa cagttgccag tgccggagca atggtcgtgg tgaggaagat
     1621 ggtgcccagc tgcggaaccg atgttgcagc agcgccgttg aatccatacc agccgaacca
     1681 caggatgaat acgcccaggg cgccgatggt cagcgagtga cccgggattg cgttgacttt
     1741 taccttgccg tccttggtct tggtgaattt accgatacgg ggtccgagga tcgcagcacc
     1801 gatcagtgcg gagataccgc cgaccatgtg gatgacaccg gaaccggcga aatcgtggaa
     1861 cggtgtggaa agctgagaca gccagccgcc gccccatacc cagtgtgtct cgaccggata
     1921 gatgactgcc gagatcactg cggaatagat acaataggaa atgaactttg tacgttctgc
     1981 catggcaccg gaaacaatcg ttgccgctgt tgcgcagaat accaggttga atacgaaact
     2041 ggaccagtca aagtttgcat agtccgagaa aatgtcgaaa ccaggcttgc cgatgatgcc
     2101 gaccagatct tcgccgcaca gcagaccgaa accgatcaga atgaatacca ctgtgccgag
     2161 acaaaagtcc atgaggtttt tcataataat gttgccggcg ttcttcgctc tggtgaaacc
     2221 agcctcaacc attgcaaaac cgcactgcat aaagaatacc agtgccgcac cgaccaggaa
     2281 ccatatgccg taaacgttgt cgtttatcag acctaaaatt gaattttgat ccataagaat
     2341 gcacctctcc atgatgtgat ataggggaga gcagcggtac cgttctgctc tcggtctctt
     2401 ctgcgattct cctttttttc ctcgcaaaaa agaaaacagc aaaggctcta cgcatttctg
     2461 cacagcgcct ttgctgtttg tgctttcagt atagacgatt ttttcccggt tgtcaaggct
     2521 tttgcacgga ttcttgcaaa tcaagaaaaa atcgaagttt cactttgcaa aacggaaaaa
     2581 tttatccgtt tttcttttcc ccgaaggaat ctgccaggat ctgagcggtt tttgtcagat
     2641 acgggacatc tgcctgtgcc agagcgccgg tgtcgcaggc tgcggcaact gccggatcca
     2701 gaggaatacg ctccagaaca ggcacattgg tgctgttgac tgcgtcatcc gagccgaaca
     2761 gcggaatttc cttgccgcag tccggacact tgataaagcg catattttcc accataccca
     2821 gcaccgggat atccatgcgc tgtgccatgg agaccgcttt ctgtacgatc atggagacca
     2881 gagactgggg tgtggaaacg ataatgatgc cgtccagcgg caggctctgg aatacggtca
     2941 gcggcacatc gcccgtcccg ggaggcatat ccacgaacat gaagtccaca tcgccccagg
     3001 agacctcttc ccagaactgc tttaccacgc cggaaatcac cggaccacgc cagaataccg
     3061 ggtcagtctc gttttccaac agcaggttca gcgacatgac ctttgtgccg tctgcggcaa
     3121 tatagggcag caggttctgc tcgtcggtct gggcatggtc gtggatgccg aagatcttcg
     3181 ggatagacgg tccggtaata tcggcatcca ggacagcggt ctcatagccg agccggcgtg
     3241 tctggatggc gagcaggctg gtcaccaggg attttcccac gccgcctttt ccgccgacaa
     3301 caccgatcac ttttttgaca ccctttgtat tgttgatggc acgttcgttc tgttcttttt
     3361 cctgacatcc gccgctggtg gcgcaggaac tgcattcatg attgcaatct gccatgattt
     3421 tttacgctcc tttttgatga ttgtccgcct tggcggaaca actttattat accacatttt
     3481 gcggtttctt gcaagtcact atggaaaaca atagattccc ttgtcccttt tcaaggtgac
     3541 tggacagcaa tgtatgctga atgcacaaat ggattggatt tctctgaaac agcagcggca
     3601 ttataattcc gcatacggca gggcatccgc atcgcttaca gtgaaaaact gcatcttctc
     3661 ccggagcgca tccgccagca tttgtaggcg ttgtgccggt tcaccggcag gaatgtggaa
     3721 attgtccaga atttgtggaa aggactttgc cggaacgaag tcccacgatt ccggagtccg
     3781 gtcgtctccg gtgaacaaag gcagatagct gcccttgcag gggatgccga tgtcgtagtt
     3841 gtacaggctg ccaatctttc cctgtgccat tcccagcaga tgtccgtatt tcagacagcc
     3901 gatggagacg gtgcggtctg accggacgca gaacccgtcg ccggaatcgc cgttcatgcc
     3961 gctgttgata tccacgctga ggacagccgc accggactgg ttgatctgct ccacgatatg
     4021 ccgcatagaa gggcgcagtt ctccacgaaa tcctgtcccc agcaggcagt ctacaaggat
     4081 gtccgtctgg gaaaagtcca cgctgtccgt gacgggaatg ccccgttctt cacacttggc
     4141 gaagaaataa ccgccgtccg gggaaacttt ttcgctggtg cggacgatcc ggcaggggat
     4201 gccgtgctgg cggagcagga gcgccagcac atagccgtca ccggcgttgt tgccgctgcc
     4261 ggtgacgatg tcaattttgc cgtgccagtc ccagctctga aacaagccta gcccggcacg
     4321 gtacatcagg gtcttgctgt ctgtgccgcc ggcgatctct gcggcatcgc tggtacgcat
     4381 gacggcaacg gaaatgagtt tttccatgag aattcctccg atttcaaaaa aggcaacgcc
     4441 attccgaaga acggcattgc cctgtttttt gcttttatct cgcttactgc aagccagcca
     4501 gtgtggtgaa ctgtgctttt agagccgggt agatctcccg gtacagctgg taataggact
     4561 catataccgg aacattttct gcctgcggtg tctgagtttt gtcggtcttg acgatagcgt
     4621 cacaagcctc cggcacactg ctgtaaatgc ctgcgccaac cgatgccagg atcgctacgc
     4681 ccagagacgg accttccttg gatgccactg tcttgaccgg gcagttgtac aggtctgcca
     4741 gcatactccg ccacagggga gatgtgccgc cgccgccgca cgccatcatg tcatctacag
     4801 agatgcccat ttcccggaat acctccacgc agtcacgcag ggaataggtc acgccctcca
     4861 gaacggcacg gagcatatcc cgcttggtgt gcattgcaga aagtccgaag aatacgccac
     4921 gtgcgttggg atccaggtgc ggtgtgcgct cacccatgag atacggcaga tacagcagac
     4981 ggtttgcgcc aaccggaacc tgttctgcct ccttgtccat aaggtagtat tcgtctacgc
     5041 ccatgaggga tgcggtctgc ttttctgcgg cgcagaagtt gtcacggaac catttcagcg
     5101 acagaccggc gccctgggtc acgcccatca cgtgccagct gttgggaaca gccgcacagc
     5161 aggtgtgtac tctgcctttg tcgtccattg ccatggaaga ggtgtgggcg aataccacgc
     5221 cggaagtgcc gatggtggtg aacgccttgc cgtccttgac tacgccggtg cctaccgcag
     5281 cggctgcatt gtcgccggcg ccgccgacaa ccacagttcc ctcgcacagt cccagggtgt
     5341 ctgccatttc acgggtcagc ctgccggtca cttcgcagga ttcatagacc ttgcccagca
     5401 gtgccttgtc gatctggagc aggcgcagca gttcgtctga ccagcagcgg tttgccacat
     5461 ccagcagctg cattccggac gcatccgaaa cctcggtggc atactcgccg gtgaggatca
     5521 gacgcagata gtccttgggc agcaggatgt gacggcactt ttcgtacacg tccggctcgt
     5581 tgttccgtac ccacaggatc tttgcggcag tccagccggt gagcgccggg ttagcggtga
     5641 tctccatcag cttttctctg cccacaacac ggttcatttc gtccacttcc gcagcggtgc
     5701 gctggtcgca ccagatgatg gacttccgca ggacgttgtt gtccttgtcc agcatgacca
     5761 gaccgtgcat ctgtccggaa ataccgatgc ccttgatctc ttccttggac acgccgctct
     5821 tggtcacaac ggtctggatg gtgcggatca tggcatcctt ccagtccgcc ggatcctgtt
     5881 ctgcatagcc gtttttcggc tgatacatgg gatattcgat ggtggcactc gccataacgg
     5941 tgccgtcttc ccggaataaa actgtttttg tgccgcttgt gccgcagtct acgccaatta
     6001 cataagccat aatactgatt cctttctatt tcacgctgtt tacacgagtt ttgctggttt
     6061 tattttaaca aaaaacaagg tttttgtcaa gcattcttct atgggtatcg tatattttga
     6121 aaagttcaga tttgtacgat tttgatattc cggaatgact ttcattttgt tttggaacac
     6181 tttttgcaat gcggctggaa tgggagacgg cggaacgtgt gctggtgcat acacagattt
     6241 tccggcagct gcatcttgca tttttcggca cagtatgcta taatagactc accgtccggg
     6301 gcgatggcat ccgggcgtgt gcatttcaaa aaacggagcg tgaaacatat gcagtattct
     6361 tatacacccc atggcgtctg ttcccgtgcg atcctcattg acgcagagga cggcatcgtc
     6421 aataaagtgc agttcatcgg cggatgcagc ggcaacaccc agggcatcgc agccctggta
     6481 gagggaatgc cgctggatga agtggtaaag cgtctggagg gcatcaagtg cggcagccgt
     6541 ccgacctcat gtccggatca gctggcaaag gcactgaaaa aggtggcaga ggagcagtaa
     6601 gctttcctgc cggaaaagaa aaacggggca gggtttctga aaatggaaac cctgtcccgt
     6661 tttgtgttgg cataggttat ttgatccacc actccggcgt gatctcgccc agcttgacga
     6721 tgcgtccggc ggcgtagtcc atcatgatct cgatgtcctt gtacctgccg tttggcatca
     6781 gctgcaccat aagttcccga cacgccccgc agggtgcgcc gccggaaccg tccggcagaa
     6841 tgcaaagcac cttgtcgatc tcctgttcgc cgttggtaat catgttaaaa atggcgtttc
     6901 gctctgcaca gatgcccagc gtggagcagg tgtcaatgca aacgccggtg tagatcctgc
     6961 cggatttgga taacaccgca gcagccacgc cgccggcaag cacatagtcg gagatttgcc
     7021 gttcctgctg taccgccttg gcagcggcgt agagctttgc ccagatttcg tccatgggga
     7081 atgcctcctt gtgagcggaa atgatttgat ttagcggata agtttttgaa tctgcaaaat
     7141 aagttgagga agttcctcga aaacaacatc tgaaatgatg ctccagtttg taccatcata
     7201 atcatgcaca agacggtgac gtaatccgga gatcattccc catggaatat cgctgtgttc
     7261 cgctttgtat tcttttgaaa tatagtatgc gtgttctccg atattgtaaa gtggtgttgt
     7321 gaccagccat tgcaatgcgt attcacgcat aagcttttct tttgtgatgc cattttcctc
     7381 gatgtagtca ttgagctttt tagaatacat gagaattttt tgtatgcgct gttcgtcaga
     7441 atacttcatg caacaagcaa cctttcccgc ataatggtgt caaaaaatgt gctttcccgg
     7501 ttcacctcat ggatttcaaa aacgtctact tctttttgaa aagcctcacg aagttcctct
     7561 gccagagaga aaatcatcgt caatttgaag tctttgccgc caaagacaag aaaatcaagg
     7621 tcactttctg tggtcgcttc atttcgggca taagagccaa acaaatagat ttcttctgca
     7681 tgatatttca aagcgattgg ttttacgatt tctttgattc tgtctatggt gaaaacttcg
     7741 tttgacatag aaccagctcc tttctgcacc acccgatcta tcggacactc attcccgata
     7801 cccggcaccg aaaacttcct tttcctggtt tacatagaga atgatcggac cgagcatact
     7861 atcttccgtt gtggaaaagg tgaccgcata atagggaccg gttccagtct cttccgccaa
     7921 tgcggcgtag atgttttctg acggcggatt tttcatatcc tgcacatccg gtgtgtccat
     7981 gtcgaggatg ctcgccaggg tctcgtcagt ctcgttgttc tgcaaaaaat cctggcagat
     8041 gcggatgatg tcggaatgcg cattttcaga aaaagatgtt tccacggcac tggtggtttc
     8101 gtttgcggtt ttggacgaat ttgttccgtt gctgcacgct gccagtgagg ccagcaggaa
     8161 tgctgcaagt gtcagtgtaa atgcttttct tttcataata acacctccgg ggatattttg
     8221 gggaatagaa actagtccct tgtatactgt tttaattatt ctcattaaat tgctgaataa
     8281 catttataat tttatcaaag cattcttctt ttattttctt attaccctct cttctattct
     8341 tcatttttcc ttcattaaga caattat
//
LOCUS       DAWENO010000086         8357 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3515_length_8357_cov_10.676584, whole genome shotgun sequence.
ACCESSION   DAWENO010000086 DAWENO010000000
VERSION     DAWENO010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3515_length_8357_cov_10.676584"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3400..3409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1760 a         2033 c         2430 g         2124 t
ORIGIN      
        1 gcgccagccg ttttgtttcg gaggaactgg gaaagtcctg cggctgtccg gcgcagattc
       61 tgcggaatgc ggtgcggttt tgtctgctgc tctcggtgcc ggtgtcggct ggcattttcg
      121 cttttgccga gccgctgagc cgtcagttcc tgcaaagcac agcgatggcg caggcagtgc
      181 ggcttatggc gctcctgctg ccgctgggga gcatcagcgc ctgtctgaaa ggatatttca
      241 atgccgtgtg ccgtgtgaca gtgacagccg gctgtgatgt ggcagagttt ctgctccgtg
      301 ccgggctact gaccgggctt ttgctgtggc atcgtgccgc aaagccggaa caggtctgca
      361 cctatctggt gggcagtatg gcggcaggga cgctgttttc cacggcggtg ctactgcttt
      421 tgtatgtgcg gaaacgggag ccatgccgtg gaaaatgcag cctttccttt ggaacgtaca
      481 ttcgcctggc agtgccggtg gcgtttggcg gttgtctgac ggcggcgctc agtacggcaa
      541 atgatacgct gattccgatg acactgcggc agttcgggga cagcaccagc cgggcgctgc
      601 agcagttcgg cacatttgaa ggcatcgtga taccggtgct gttttttccg tctaccattc
      661 tctgcgcctt atcgggcatt ctgattccgg aggttgcccg tgccaatgcc gccgggaatc
      721 gggaacgggt ctgccgtctg accgaacggg tgatcgagct gacgctgatc tttgccgtcc
      781 tcatcagcgc agtgctgctg aaatacggcg gtgtcatcgg gcgctgcatg gacggcggcg
      841 aactttccgg cagaatgatc cggatccttg ccccggtagt gccgtttatc tatctggaga
      901 tcgtgctgga ggcgctgatt aagggcatgg ggaaacagaa cttctcctcc ctcaactatc
      961 tggcagagta tgtgatccgg atctgcgtgg tgttgatctg cattccgctg tttggatttt
     1021 acggcattgt ggcatcgtat tataccagca atgtgttcgg caactgcaac cggctacgca
     1081 tggcagtaaa aacggcaaac ctccggttcc gattcggaaa gctgatcgga aagccggtgt
     1141 ttgccgttgg gtttgctttt gcagcggcat cactgccgga gcatttttgt ccgcagctgc
     1201 gggaaacgcc gctgggcatt gcggtgtttc tgggaatcgc actgagcctc tacgggctgg
     1261 tgttccggct gctgcgggaa cgcagggata tcacacgtgg tgtgcctgtt cgtgctgttt
     1321 cagcagcgtg tgcatccggt gatgatggct gtgttccgtt tcactttcgc cgtgtacatg
     1381 ggcgtgactg tgggtgatcg tgtgagtatg tgtcacgccg tcgtgggtat gggtgaatgt
     1441 gtgagtgtga ggatgggtgt gttccgcctg cagggtgtcc agcacgacca gcacagagcc
     1501 ggcgagcatg actgccagcg ccaggaagta ctgcacactc agcgcatccc gccagatgat
     1561 gcaggagagc gccgttccga taaagggcgc aacggcataa taggcactgg ttttggcggc
     1621 gcccagtgca ttctgcgcac ggacgtagca gaagatgctc agcccgtatg ccacaaagcc
     1681 tagcagaagt gcggcggcga ctgcaccgag tttcgggagc ggttcgccga tgcacagggc
     1741 gatgatacag gaacccagtc cggagaagat gcctttgaga aagacgatct cgaaggtgct
     1801 tttgtcggag atgttccggg tgcagttgtt ttccagtccc cagcaggctg ccgcagccag
     1861 caccagcaga gaaccgccgg agaaatgcag gctggacata tcctcaaagg agaggatgcc
     1921 gctggaaagg gtgatgagcg caatggcaat ccagagccgc cgggagatct tttcccggaa
     1981 gagaaaaagg gcgataagcg aggtcgccac gatctcaaag ttgcccagaa gagaggcgtt
     2041 agcagaggtg gcgcttttca gccccagcat cagcagaatg ggtgcggcga tatccagcag
     2101 gatcatgccg atggtatagg gcaggtcttt gcgtgtcagc tttttctctc cggcgtgctt
     2161 tttcggattg ctgagataga ggatgccgat gccgattccg gcgccgaaat acagcagaga
     2221 cgccagaaac gtcggaccga tttgattcag cagcagcttg gaaaagggga cgtttacggc
     2281 ataaaaggct gccgccagaa cggagagcag aatggctgtg gtctttttgt tctgcatgac
     2341 ggcgctctcc tatcgctctg taatgatatc agccagctgg aacggaacga ccttttccag
     2401 gctgtccagg gaaagctcca gctggtgtcc gatttttccg gcactgcaca gaatggtggg
     2461 gaagttccgg gcggattcat gtatggtggt atggaacagc tttttcatgc cgatgggaga
     2521 acagccgccg tggatgtatc cggtgagcgg cagcaggtct ttggatttgc acatggagat
     2581 ggctttttcg cctaccgcag cggcagcctt tttcaagtcc agttcctctg ccaccggaac
     2641 gagaaagacg tagtgttcgc cggactttgc cacagtgacc agcgtcttga atacctgcgc
     2701 cgggtcttgt cccagcgcct gtgccagctc tacgccggtg tgctgtccgg tcgcctgttc
     2761 ccagcagcgg tggatgtagg ggacttttgc cttgtccagc atccggatgg cgttggtttt
     2821 gatgattttt tcttttgcca taacgatcat tctcccttga gtgtaacggt aacataggtg
     2881 tggtgcagag cgtttgttag cttgtcaaag acatcgctgg tctgcatccg gacggtggaa
     2941 gtgttgatgc agggatgaca gccgaagtac ggctcccgga gcagttcctg atccaccacc
     3001 agctgcacct ggttttcggt gtcatacatc agaccaaaga tggtggcaga gcctggggtc
     3061 agatgcagta gctgttccag cgcctcctcc ttggcgaagg acagccgtgc gcatcccagc
     3121 tggctggtga tctctttggt gtgaaagggc ttgtctcccg gcataagcag caggtaaaaa
     3181 ctggttttct gccggttgca gagaaatagg ttcttgcaga tcttggtttc cagcgccgca
     3241 gcgacttctt cacaggcatc catggtaaag gctggttcgt ggtctgcccg tgtaaacggt
     3301 atgtccagct gttccagcag atcgtataca gcggtttctt tttccagtct gccgctttcg
     3361 tctgcgggtc tgccgttttc aattgtcatt ttggaataan nnnnnnnnng tgtgaacatg
     3421 atgtcgttca tgattgcctc cagcatttcc tggcggccgc tggtcagaga atcggtaact
     3481 tcgccctttt ccagtgcata cttttccagg tcagccatgg ttgccttgcc ggaaatgatg
     3541 tcagcgccga tgccggtctt ccagcttgca tagcggtcag ccacaaagct gtccagtctg
     3601 ccgtcggtaa tcatcttgtc ggcgatgcgc agacccagtg caaaggtgtc catgcctgcg
     3661 atgtagctgt ggaagatgtc ttccagctcg aaagagccac gtcttgcctt tgcgtcgaag
     3721 ttcagaccgc cgttggtgaa accgcctgcc ttcagtactt cgtacataca aagtgctgcg
     3781 tcatatacgt tggtcgggaa ctggtcagta tcccagccca gcagcggatc gccctggttt
     3841 gcgtcgatag aaccgaacat accgttggtt cttgcaacac gcagttcgtg ctggaaggtg
     3901 tgctgtgcca gtgttgcgtg gtttgcctcg atgttcagct tgaagtcctt gtccagacca
     3961 tacttgcgca ggaaaccgat gactgttgca gagtcgaagt catactggtg cttggtcggt
     4021 tccttcggct tgggttcaac atagaagtca ccggtgaatc cgatgcttct tgcataatct
     4081 acagccaggt gcatcagacg agccatgttg tccagctcca gacccatatc ggtattcagc
     4141 agggtctcat agccttcacg gccgccccag aatacatagc cctgaccgcc cagctttaca
     4201 gttgcatcaa tggcgctctt gatctgtgca gcggcatatg cgaatacatc agcgctggga
     4261 gatgtgcctg cgccgtgcat ataacgagga tggtcaaagc actttgcagt accccacagc
     4321 agctttttgc cggttgcatc ctgcttttcc ttgcacaggt caacgattgc tctcagcttt
     4381 tcgttggtct cgctcagact gccgtattcc ggagacaggt ctctgtcgtg gaaacagtag
     4441 tagtcgatgc tcagcttgtc catgatctcg aatgcagcct cgacctttgc ctttgcacga
     4501 gctgtcacat catcacagcc ccaggtcttg tcggttgtgc cgcagccaaa catatctgtg
     4561 ccgtcgccgc ccatggtgtg ccaccaggac agggcaaact tcagctgttc ccgcatggtc
     4621 ttgccggcga tcacttcgtc cgggttgtaa aacttaaatg ccaggggatt cttggaattc
     4681 ttgccttcgt atgcgatttt cggaatgctc ttgaaaaatt cactcatggg tgtttcctcc
     4741 taaaattttt tgggagcgtg ttcacgcata cttttttcag tgaacatact ccagattatt
     4801 aatggcgttt gatacgcctt gcatccagaa caattgagaa ttagttaaat aaggagatct
     4861 ctcggttatc ttttgcgaaa taatcttcca gtacagcaat atacgagcga aaacaggtct
     4921 tgtccgcaaa ggtcagcaca tcgttttctg ccggcacgcc gagctgttcc agttgggaat
     4981 cggtcaagtc tttcaggtag accgtctctg tgtctgacgg gatgccgatg gtggctttct
     5041 gcgacttgct gagatccagt gtcacatcct ctgagattgc cagcagctgt gccgtgatat
     5101 ccgcacggga tacggtagac cactgggcgc tgccaacctc actggggatg gggcgctctt
     5161 catagactgt ccggtcataa ccattggaga cgaaggtcag aaacggcagg tggatgtccc
     5221 ggtcgttcgg ctcgatgtga accgcaacat tgccgccgga actgtcatcc gtctggatga
     5281 tctcataccg gtatgcgccg gagggcgact cgccgttttc cagaatggtc tgctgtgtgc
     5341 ttggggtaaa gaaagacatc agcatgaaaa atgccagcga acccaataca tataatagta
     5401 ggtatatgac gccaaagacg gttttcaccg tttcgccggc accggacagg aaaaagatga
     5461 taagcgacgt gacagcgatc ggcagaatca gctcccattg cccgaaacac agcaggatct
     5521 gcggcaggat cgccggcagc agaagaatgc tggtcatacg ggtcagaatc tgaaagcggc
     5581 tgtagagcat agcactcagc gctgcaaaag agacggcgca gagcgtcaga caaaacagga
     5641 acttgttcgt ggcagtaaag tcatagaaaa tgaccagata ggaaaaccag cacatcagcg
     5701 ccagatatcc cgcaccgacc agtgacgcca ggcgtctccg ccagatgttg ggcgttgttg
     5761 tttcagctgt cgtatttgtt gtactcatgt atattttctc gtctgtctga atttgcagac
     5821 gttttccttt ctttgcctga gagcgttcgc cgtctggacg aatgttcctc ttttacggca
     5881 gaaatggctg cacgtatccg caaagaaaag tttctctgtt tcttgcgtgc ttcgcactcc
     5941 tattatagca tttttttcgc attgtggcaa gtactattgc aaaaaaatta caacaatcgt
     6001 atataatcct accgtttttt gcgatgattc cggaaaatct gccgggaatg tccgcaggag
     6061 aatggggaaa agcaccgctg ttccggcgta ttttgtcggt ttttacagtt tatttataaa
     6121 aaaatatgga aacacccttg taaatttgtc ggatttgtgg tatgattgtt tttatgtttg
     6181 ctaagagtct gatttgtact gttcaagagc ggcagcgact ctggagcaat gattttggaa
     6241 aggagatagg attgtgacaa aatcagtttt gtgtattctg atcgctattc tggtgtatat
     6301 ggcaatgatg gtcgtgatcg gcgtgatata ctccaaaaag aacagcagtg tcggagactt
     6361 ttatctggga ggacggcggc tcggtcccat cgtctctgcc atgagtgcgg aggcatcgga
     6421 catgagcagc tatctgctga tgggactgcc gggactggcg tatctgagcg gctgtgcgga
     6481 ggtaggctgg acggcgatcg gtctcgccat cggtacttat ctgaactggc tgctggtggc
     6541 aaagcggctc cgggtgtatt cccagagatg cggaaacgcc atcaccatcc cggacttttt
     6601 ttccaaccgc tatcgtgaca acaagcacgt gctgatggga attgccgctg tgattattct
     6661 ggtgttcttc gtgccgtata ctgcatcggg cttttctgcc tgcggcaagc tgttcaatca
     6721 gctgttcggc tgggattacc atatggcaat gattatcagc gccgttatca ttatcgcata
     6781 cacctgcctg ggcggattcc tggcggcaag ctttactgac ctgatccaga gcatcgtgat
     6841 gacttcggcg ctggtggttc tggtggttta cggcatccac acagccggcg gcatggataa
     6901 tgtgatggaa aatgcgagag gcatcgagaa ttaccttagc tttaccagta cccggacagc
     6961 agacggcgag ctggtctctt acgacggtct gagcatcgtt tccaccctgg cgtggggtct
     7021 gggttatttc ggtatgcctc acattctcct gcgctttatg gcgatcgacg acaagaacaa
     7081 ggtcaaggtt tcccgccgca ttgcctctat ctgggtggtc attgccatgg gcgttgccat
     7141 tctgatcggt attatcggca atgcgctgac tgcaaacggc acagttgcac cgctggaaac
     7201 ggcgtctgca tcggagacca tcgttctgga aatggctact gtactcagca agcacagcgt
     7261 aggtgcggcg ctcatcgccg gactgatctt cgccggcatc cttgcctgca ccatgtctac
     7321 ttcggactct cagctgctgg cggcatcgtc cagtatgtcg gagaatctgc tgaaaggcgt
     7381 gttccacgtc aagctgtcgg aaaagcagtc catgctggcg gctcgtctgg ttctgctgat
     7441 tatcgctgtg ctgggtgtta ttctggcgtg ggatcagaac agctcggtgt tccgtgtggt
     7501 atccttcgca tgggcaggat tcggcgcaac ctttggtccg gtagtgctga cggcgctgtt
     7561 ctggaaacgt tccaacaagt acggcgcact ggctggcatg attaccggcg gcgtcatgat
     7621 cttcgtgtgg aagtttgcca tcagcaagct gggcggtgtg tttgccatct atgagctgct
     7681 gccggcgttt ctgtgtgcgc tgatcgccat tgtggtggtc tctctgctga ccaaggcacc
     7741 ggaacaggag atcgtggacg agttcgaggc ggtctcttcg gagatgaaac agaagtaaaa
     7801 agctgacaag tgaatattgc aaaaagctgg tttctgtatg tgcaggggct ggcttttttg
     7861 ttgcagaaat gaggaagaag agttgacatt tctggatcaa tgtgatataa taatggtatt
     7921 atagattgct aaaaatgata tggaggaatg gcaaaatgaa gttatggaaa aaagttcttg
     7981 catcagtgac cgcaggcgtg ctttgcgtgg gcggtgcggg gctgtctggg gtggagagcg
     8041 tgcaggaaag catgggaacg gtgttatcag cgagtgctta cgatggcatc acatatgaag
     8101 gtctgtatta taatgtgacg gatacaggag aaattgaaat cgctagatgt tatgaatatg
     8161 ttagaaaagt aaatattcct atagaaatcg atggaaaatt cgtgacgagt atcggaaagg
     8221 aagctttttc caactgtacc ggtttgacgg aaatcacgat cccggacagc gtgacgagta
     8281 tcgaagatta tgctttttcc aactgtacca gtctgacgga aatcacgatc ccggacagcg
     8341 tgacgagtat cggagag
//
LOCUS       DAWENO010000087         8316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3529_length_8316_cov_9.044184, whole genome shotgun sequence.
ACCESSION   DAWENO010000087 DAWENO010000000
VERSION     DAWENO010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3529_length_8316_cov_9.044184"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7363..7433
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2419 a         1764 c         1950 g         2112 t
ORIGIN      
        1 tgtaaccggc ggtcttgatt attattttaa aaaggcaggc atggcagact actatctgtt
       61 cgctcacgac agcaatggtg aaggaacggt agaaaatctg cttgaaacaa gcgccaatgt
      121 taaggactgg cgttctgaaa aaatgataga tgcaacatct gaggcgcttc gtgacggagg
      181 aaacaagttt atggatttct ccaatgtcct gctgatatgc gatatttccg acaccaatct
      241 gaatttcttt gacagagaca acaatatcgt cagtgacgtg ccgaagggca aggtgtatat
      301 aacagggtat gtgccgatca gagagggcgc tacagccggg gataaattca gattgaaact
      361 cgacagtacc gagatcggac ttgaagttga aggtatcgca aaagatgcgc tttttggttc
      421 tgcaatgatg gcaaatccac gattgcttat gaaccacgag gactttgaaa cactccttaa
      481 agacgaaaca ataagcacat acagaatcgg acatatttac tacattgatt ccgataatac
      541 atctgcactg gaatcggata tttcagggct ttcaggcatt tacttttccg gcagcaatgc
      601 tatgataaaa acgacctata tcatgaatac gcttgtggca atggtgattc ttgtcatcag
      661 cataggtctg attatcgtgt cgttcgttgt gctgaaattt acgataaggt ttaccatttc
      721 agaagaattt cgtgagatcg gcgtaatgaa agcagttggt ctgccgaata cttcaatacg
      781 ttccctgtat atcgtaaaat atttcgggat agcgattgtg ggcgcaggca tcggatatgt
      841 atgcagcata ccgttcggaa aaatgctcat caacagtata agtcaaaaca tgatgcttgg
      901 aaatgacaac agcattattg taagtattct ttgctgtatc gccgtagtac tgctgatcct
      961 cctgttcagc tggcaaagca caagaaaagt gaacaaactc tctccgatcg atgcagtccg
     1021 caacggtcag acgggcgaac gctttgggaa aaagagcatt cttcaccttg gaaaatcaaa
     1081 gctgccgaca agcggatttt tcgcactaaa tgatgttgta agcgagccga aacagtacgg
     1141 tatcctcaca gcggtattcg cagtatgcac atctatggta atgatacttg ctgtcacggc
     1201 aaacacgctt aacagtgaca gtctgttata cctgctctct gtgacaaaca gcgatgtgta
     1261 tatgtctgat tcagaaagag cgcatgacgt tatgacaggc atcaaaacgc tggaagaaac
     1321 agaaactgag atagaggata ttctcgctga acatgatatg cccggaacgg tgtatctgga
     1381 gacatggtat aaactgccgg tcacatcaaa gaatgaaaat ttcaatattc cctttctgaa
     1441 atgcaaggat acaaagacga ccgattatga atacacggac ggcataccgc cgatgtgtac
     1501 ggacgagatc gctgttacag agcagatcgc cgataagctg aatgcacata taggcgacca
     1561 tgtgacggta aatattggta atgagaaaaa ggagtttatc ataacggcat acttccaggg
     1621 tttcaataat cttggcgaat gtgcgagatt tcatcaagac ctggaaatcc ctgatacgct
     1681 gatgtcttct gcattttcct atcagataaa ctttgacgat gccccggatg atgcccttat
     1741 ttcggaacga atcgaaaaaa tgaaaaagat atttgacaat gaaaatatca tggatacaac
     1801 ggaatttgtc atggaaagta caagtgccgg cgagacgatc cgttcggtaa aaaaccttgt
     1861 gcttgccatt tcaactgtca tcatcatatt gctggcagtg cttatggaac gcagttttat
     1921 cacaaaagaa aaagcagaga tcgctttgat gaaagcagtc ggtttcagtg acgggtctgt
     1981 tattctgcat cacaccttac gctttaccgc aattacactt atcgcaatca tgatctcatc
     2041 ggcattgctc atgccgctta tgaatctaat catagatccg atatttgggc gcatgggagc
     2101 aacgggaaaa atagcctatc agataaatgc cgcagagata ttcggcttat accctgtcat
     2161 aattctcaca gctaccgtga caggcacact tctcactgca ctctgcacaa aaacaataaa
     2221 agcaagcgat accgcaagca tcgaataatg gaggaaaatc cgatgaatac agttttacag
     2281 acaaaaggac tttgcaaaac gtacattgta aacaagcgcc agaacaacgt cctgaaaaac
     2341 attgacctga ccatccacga gggcgaaata gtagcaatta tgggaccgtc cggttcgggc
     2401 aaaagcacac ttctctactg cgtttcgggc atggacagag taacagcagg caatgtcttt
     2461 tttgacggca gagagatcac taaacgcagt gacaaggagc ttgcacgact ccgcctggat
     2521 gaaatgggat ttatctttca gcagatgtac atgatgaaaa atctttctgt actggataat
     2581 atcattctcc cggcggtgaa atccaagaag aacaccgaaa cacgaaagca gatcgaggag
     2641 cgtggacgga cactcatgca cagactgggc attgacgata tcggcagcaa cgatatcaat
     2701 gaggtatccg gcggacaact gcaaagagcc tgcatctgcc gcagtatgat aaacgatccg
     2761 aagatgattt ttgcagacga gccgaccggc gcactcaacc gcaaaagttc cgatgaagtc
     2821 atgaacgaac tgctttccct caaccatgac ggcacaacgg tcatgtgtgt cacccacgac
     2881 ccgaaagttg ctgcaaagtg cagccgggta ctgtacatcg tggacggcgg tatcatgggc
     2941 gaaaaggaaa tgggtcattt tgacggcagc gataaggaac tgcgtgaccg tgaacgtgac
     3001 ttgaataact ggctgatgga acaaggctgg taaatcgcaa ggggcagaat ttcggttttg
     3061 ccccttgatt tcgcactcaa tttatggtaa aatatttaca ggtgatgaaa ttatgataga
     3121 catactgatc gttgaagatg atacagaatt atctgcgctg ctgaccgatt tcctgcgtaa
     3181 tgagggatat actgtttcag cagcggcagg cggcgaatat gccattgcgc tttttgaaaa
     3241 atacggtgca aggcttgtga tcctggacat caatctgccg gatgtgaacg gctttgcggt
     3301 ctgttcaaaa ctgcgtgaaa atgcggacac gccgatactg atcgtcagtg caagaacgga
     3361 taaagcggac aagctgaacg gctttgacct gggtgcagat gattatatcg aaaagcctta
     3421 tgatatagac attctccttg caaagataaa aggcattttc aagcgcagat atcagcgcag
     3481 cattctttct gtgaacggcg ttacgctcaa tctcgccgac cgcacggcag aaattgacgg
     3541 caagcctgtc gaactgccgg caaaggaatt cgaccttctg caactgctga tggaaaatca
     3601 aggcaaggca ctcaaaaagg aatatctgtt caacaccgta tggggcagtg acagcaattc
     3661 ggaattgcag acacttcatg tgcatatcaa ccgactgcgg cagaaattag gcgatgatcc
     3721 gaaaaatgca aagcggctga ttaccgtctg gggtgtgggg tacaaattcg tatgaaagca
     3781 tttcgtaaac tttgcataac gataacggca gtatttgtcc tgcttgccgc agtgctgaat
     3841 gtatgccttt tgcgcacgga taacagcagc ggcatctacc gtgtggaagt gaaacggctc
     3901 accgatgaaa taacacagac tggttcttat gacctgtcgc aatatccgca tttaacaggc
     3961 gtttttacag gcgacgactt gtacacctcc gatgagccgt atgtcatcat tgatgggaac
     4021 gatacgctct atcgtgtgga atatacgacc gaaaataatc atcacgattt gatgatcgtc
     4081 aacagcgtgt tcggggcgat ttttctgata ttgatgtgtg tgcttttgta catcaggcaa
     4141 catatcattg tgccgttcgg cagactgaat gatgtgccgc aggagctggc aaaaggaaat
     4201 cttgcggtag agataccgga ggaaaaaagc cgctttttcg gcaaatttac atggggcata
     4261 aatcttctgc gtgaaagcat cgaggacagc cgcaaaagag agttgtccat gcagagggat
     4321 aagaaattac tgctcctttc actgtcccat gacatcaaaa caccgctttc ggcgataaaa
     4381 ctgaatgcaa aggcgctcga aaaagggctg tacaaggaga aagacaaaca gcttgcggcg
     4441 gcacaaagca tcggcaaacg tgcggacgaa atagagcagt ttgtcagtga gataaccaac
     4501 gccgcaagcg aggacttcat gactttcgag gtcacccaga gtgaggtgtt tctaagttcc
     4561 atcctcgcaa agataaatgc aagatacgcc tcacagcttg cattatcggg aacggagttt
     4621 gccatagaga actacgatga ctgtattctc tcctgcgatg ctgaccggtt ggcagaatgt
     4681 ctgcaaaatc tgatcgaaaa tgctataaaa tacggcgacg gcagacaaat caaactgagc
     4741 tttgataaag ttgacggctg tgagctgata acagtcagaa acacaggctg tacccttgaa
     4801 gaaaaagaac tgccgcagat atttgagagt tttcaccgtg gcagcaatgc agctaaagtg
     4861 cagggaaacg gtctgggact gttcatctgt aaacggttga tgtcactcat gggcggcgag
     4921 gtctatgccg aaattcgaga gaaatgtttt tgtgtgacgt tggtagtgaa actggcgtaa
     4981 aaattttata tagaggaggt cacagcagtg acagacaaga aagcgctgac gctattgaaa
     5041 aaatactatc tcccgtacac ggcagaggga acgctgtccg aatctgacag agcggatgcg
     5101 ttcaaaagcg gtatccttgt gccggattcc gctatgacac acgatgaaat cattacagcg
     5161 atcaaatctc tttctgaaga tatttccctg gaaagcactg caaaagcatt cctctacagc
     5221 ctttcaagcg gagatatgcg atatcgtaca gctttatcca gtctcgtgtg ggcaagatca
     5281 cttccgaaac acgaattcat atccaataac gcagaaaagg cgggatggcg cacgccgtcc
     5341 tgttcggtat gcggctgtac acatgggctg gtaacaacag agcgcattga ctggaacaat
     5401 tacggcgttt tccgttactt tccgccaaaa cagtacggca gagagccgga tttcgcctgc
     5461 gccgagtatg tgctgcacga tctgcgtgag tttgaaaagc tgcctgcggc agaaccgtgt
     5521 gaagaagatt attgtatact gaacggaatc tttgcctgtg tgggacaaat gaaaccgcac
     5581 aacatggaca ttgctttggt gaatgagata cggggcaaga agtttctgga tgctacgggc
     5641 aacgctgttc actgtatttt gggaattctc tctctatgtg gcatactgga aagtaccgca
     5701 cataggggct ttttgcataa ctttacaaac agcggcgaga tcggcggtta tcgtgacggg
     5761 ctttcgtttt atcccctatt ctattggcgt ggaaaaaatg gagtgaatta tgacgctgta
     5821 aatgaaatat tcggcagttt cagcggcgac aggttatctc ccagtcatgc tgttatccct
     5881 gtgaagaacg aggcaaaagc acctgcaaag aaggttgttt caaaagcgga accgtattat
     5941 tctgacggcg tttacacgat catgcttacc gatgcggaac gacggtatct tgcacttgat
     6001 ccaattgatc cgagctggga aacagaatct atgtacagcg tgacgcattg gctgaaaaag
     6061 cgaaccgtct tattttataa ggaaaataca atcgtcaagg tgatccatga agtgcattct
     6121 atagatgatg acggcgcata ttgctggaga gactacagcg agtttgatac ccgacttgaa
     6181 acagacaacc ggaaaatgct gctgccgctg actgcaaggg gcagagcgaa accgatcacg
     6241 ccgaccaatg tgatggcagt caaaccgttc ggctgtacct tcaatattta tctgaaaaaa
     6301 gaggaaagtc agatagcggt tcagaatctg agaaataatc agatcattcc catcggagaa
     6361 gaagagcgtg tgaaaaaaat catgtctgat gaagatttcc atgaatttat gcgttactat
     6421 atttcaactt gtcccgatga ttattttgag catatctctg cggtaaggaa tatgaaacac
     6481 cagactgtga aattcagagc aggcgatatt tttcgctgtc agatcgacag aacacactgt
     6541 gcctacggac tgattctcgg aaaaaccagg gaaattgaaa cgtggaagga actgcccaaa
     6601 gagcattctt ttcgtcatgt catgacgcag cctattcttg taagaatgta tgattttgtc
     6661 actacagatc aagagatgtc agcagctctg ctttcagaca aaactttgtg tccgccgaga
     6721 ctctgctcgg actgtgatat attatggggt acacataaaa tcgttgctca taaaaagctt
     6781 gtccccgatg atattcaatt tcagttacag ctcgcaaggc aggtgaaaaa ggacgagcat
     6841 tttacacctt ttacggcgga aggctttatg gaaatgtccc caagccgtcc gaatccggaa
     6901 agcttatacg tagagtgggg atttgcttcg tttgagatac cgtgggcaga cgttcccgac
     6961 aacatccggc gtctgcttga taaaggaaga tactttgacg gcggtgttgc aatgtttatc
     7021 aacgccgata cgccaaagca tatacgccag gacaacttgt tattgcttga aaatcgggac
     7081 aaatttggtg ctgttatgga gttcctcggt ctgtctccca aatgtacggt cgacgatttt
     7141 gccgctaaat atggcggaat ctcacggcag aaatatattg aactgattga ggagcgaggt
     7201 aagtgaatgt gacatgaaca tggtaaagca ttgctttgcc gtgttttgtt tttctcatac
     7261 aaaacagaaa aggcagtcct gcccggctgt atgccggaca gagctgccct ttttcgtaat
     7321 tgaattttaa ggatggtcaa ccacactggc gtattcatct aannnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcttgcc
     7441 gttcatatcc gatttctgta agtcgaatat cgttaatcca ctgaaaatag tgcgtcaatt
     7501 cgtgtgctat tgtttccagt atcagcgcta aagcatcatc tctccctagt gttatcaaat
     7561 tatctgtata acttccggtt gcaacacgta tatatggttc atacatctcg tgatatggct
     7621 gaaaaaaagt gccatatacc atttcgccgt ccatcgactt aatgtatttt ttccctttca
     7681 catacaccgg cactcttatt gggaaaaagt actcttttct caaccacttt ccaaatttca
     7741 ggcacgcttc tcttacttcc gtgttaacgt cggaggtaaa acggaatcta agaccacttc
     7801 ttcttgattg aatatttgta aagtgatatt tccaattctc aagtgtccag atatgcacaa
     7861 tgtcacttcc tgtttttcag cttaaatttt ctgctggatt cctgtaatat ggttgtttgt
     7921 tgttctttag tataccataa ttttgagaag aaatcaagaa gtaccaaaag caaaatagcc
     7981 gaacaatgcc accgccgatt atctacgaaa taattgtcaa ttttccctta tccatctcac
     8041 aaacggtatc gcacagcaca gaaatgtctt ccgccgaatg agatgccaaa agaatggttt
     8101 tcccctgttc ctttagatgg agcaggtatc ttcgcatatc ctgtacgccg tctttgtcca
     8161 atccattcat tggttcgtcc agaatcagca ggtcgggatc ttccatgatc gcctgtgcca
     8221 atcccagtcg ctgccgcata cccagtgaaa actttccaac gtgtttttta ctgtccggat
     8281 ctagcccaac cgattgcatg gcatttcgga tttttt
//
LOCUS       DAWENO010000088         8175 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3605_length_8175_cov_11.633621, whole genome shotgun sequence.
ACCESSION   DAWENO010000088 DAWENO010000000
VERSION     DAWENO010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_8175_cov_11.633621"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2272..2281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1962 a         2244 c         2375 g         1584 t
ORIGIN      
        1 tccaaggaat ctgtgggacg gcgtttttgc cgtctgagcg agaaaggagg tactgttttg
       61 aagagaacat atcagccgaa gaagattcat cggaaaaagg tgcatggctt ccgcaagaga
      121 atggccactg cgaacggtag aaaggtttta gctcgcagaa gagcaagagg cagagcaaga
      181 ctgagccact gatcgaagtc ggaccacaac gccattggtt tgtggtcttt tgttttttcg
      241 aggagcgtat cctggattcg ccgttgtgcc gattgcggaa aactgaccga taccgccaag
      301 gtgcaggtca gaggatccgc agccggtgcg gcgtgatgcc ggggtacgct tttgcttttc
      361 ccgaaaagac agccgggcag acggttttgt ccggctgaaa accgaaaaaa gttggagtat
      421 aagccatgga aaaaacggtg attctcaatc aaaaccggga cttcttgcag ctgtaccagc
      481 gtgggaaaaa gatcgcatcg ccgtatgtta tcatctacct gcgcaagacc agacgcaagc
      541 ataaccggct ggggattaca gtgggaaaaa aagtgggttg tgccgtaaag cgcagccggg
      601 ctcgccgtat catccgccag gcatatcgtg agactgagca tctcatgccg gtgggcgtgg
      661 atatggtgat cgtggcgctg ccgtccatta cgggcatacg ctctacacaa ctgcgtgact
      721 atctggcagg gagcggcgtg cgccgcatcc gaaagctggt ggaaccgccg tcggaaggag
      781 gaaaaccgaa atgaggtacc tcgtggcggc gctgatccgc ctgtaccaga aggtttcccg
      841 actgactccg gctgtgtgcc ggttttatcc gacctgttcc tgttatgcac agactgcagt
      901 tctgcggttt ggtgtggtgc ggggcggctt tctcgccctg cgccgtctgc tgcgctgtca
      961 cccatggaat cccggcggca ttgactacgt tccggaatct ctggaaccgc cccgggctgt
     1021 tttcgggcgc tatgtgcgga cactaagaca caacagaaag ggttaaaaca aaactatgat
     1081 ggatttttta tacgatctgt gcggaaagcc ttttggctgg atcatgcgcc tgatctatga
     1141 ctgggtggac aactacgcat ttgcgatcct gctgttcacc atttttacca agctcatcct
     1201 gttccccatc tcttataagc agcagaagaa ctccgcaaga atgcagcggg tcaatccgaa
     1261 aatggaaaag ctgcggcaga aatataagga tgatccgaaa aagatgcagg aagagcagat
     1321 gaagctctac caggaagaga acgtgaaccc cggcgcatcc tgtctgccga tgttcctgca
     1381 gttcttcatc ctgttcggca ttctggacgt tgtctataag ccgctgtcct atattctgaa
     1441 aatgaaagac agcgtgatct ccagtgcctt tgaagtggtg cagaagatca atccggaact
     1501 ggcaaagcag ggcaccagcc tgcggcgtga gctgaccatc ctgactgcgc accactccga
     1561 tgccagtgca ttccagagcg tgctgggaga caacctctct gcaaagatct cggaattcta
     1621 cgacaagttc agtctgttcg gcatcaacct gggcgagatc ccgacctggc atccggacac
     1681 ctggaacgcc gcagccatcg gtctgttcat gatcccggtg ctgtccggcg cactccagct
     1741 ggcgttgact atttatatgc agatctccca gaaaaagcgc aaccccggcg caccgaacat
     1801 gggctgtatg aacgtaatgc tctacgcaat gcccatcttc tccgtctggt tcgctttcca
     1861 ggtacctgcc ggcgttggct tctactgggt atgctcgtcc ttcttctctc tgatccagag
     1921 catcggtctg aactgctact tcacaccggc acgcatcgaa aagatctgcg agcaggagac
     1981 caagaaaaac gccaagaagt atgccaacgg caaaaagagc tttatgcagc gcatgatgga
     2041 tgcgcagcag ggcatggatg tccagaacca gcgtgaaaaa tacgccgaag agaccaagga
     2101 tatgtcccgt tcagagctga acaagtataa ccagcaggtg ctgaaagaag cacgcaagcg
     2161 gctggcagaa aagtacggcg acacctatga cgaaaacgag agcgacaagg attaaagggc
     2221 tgctgtgatg acgccggtga aagcgccgtc cgcccgaaaa aaccatttca cnnnnnnnnn
     2281 ntgtgatatc ccatgattac gaaagggatg aatgagaatg aaagagattt tcactgcaaa
     2341 aagcatcgaa gaggcaaagc atctggcggc cgaaaagttc ggcgtttctg aggaccagat
     2401 cgtttaccgc attctggaag agccgaaaaa gggactcttc ggcaagataa agcgggaggc
     2461 acaggtagag gcagtgtatg aagaaccggc agcggctgct gcgccggaga ccccggcagc
     2521 accggtttcc cagcccgtac aggaggtagt ttcttctgta gaggagatcg tcattgacaa
     2581 ggatgacaat tctcccacac gtggcatgga gtccgaggcg atcgaggata tcctgacaga
     2641 ggagaacatg acccccggcc tgctccgtgc aagacagtat attgcagaca tttacgcagc
     2701 aatgggcatt actgtgacca ttactaccat gcgcaccgca aacggcatcc gcatggaact
     2761 gcgcagcgac aataagagcg gcaccatcat cggcagacga ggcgagaccc tggattccat
     2821 ccagtatctg gcatcgatcc tggtgaacaa ggacagcgag gactactgcc gtctgctcct
     2881 ggacagcaac ggctatcggg aaaagcgccg caagaccctg gaacagctgg cagaaaagat
     2941 cgcccggagc gtactgcgca gcggacgttc caccacgctg gagccgatga acccctatga
     3001 gcgccggatc atccacagca agatctccga gatcgagggt gtgacctcca agagcgtggg
     3061 cgaagatccc taccggaagg tagtcgtatc ctccaccacc ggcagacgca gcggcggaaa
     3121 ccgtggcggc agaggcggtc agaaccgtgg cggcaagcgt ccgtaccggg agagaaagcc
     3181 ggaggatttc cagcgcaaga acctggattc catgaagacc agctttgaac gggattacaa
     3241 aaagccgaag ccggaggacg aactgaacgc cggactctac ggcaagatcg aattctgaac
     3301 aaggtaaatc agacagataa aacggattgt agcaatgcag tccgttttgt tgtatgcggc
     3361 agtgtgcgcc gggatgggtg tttcatgtga aacatgagga tgccgcatct gtaggggcga
     3421 acattgttcg cccgggactc ctctgactcc tccggcactg cgtgccacct cctcccttag
     3481 aggtgatgcc cgacagggca gaggagttgt ctgccctttt tgaaaagaaa aaccacaccc
     3541 caaaaaatcc gccgcaagga gaaatcacta tgtatacaat tgcagcaatt gcaacccctc
     3601 acgctgccgg cggcatttct gtgatccgga tctccggtga cagtgccgtt tccgtggcac
     3661 agacctgctt tcgcagcgtg accggaaaat ccttatccga aatgcccggc tacacctgcg
     3721 cctacggcaa attctgcgac ccgtccggtg cggagctgga tgacgggatc gccaccgtgt
     3781 tccgtgcgcc ccacagctat accggcgagg acaccgtgga gttttcctgc cacggcggca
     3841 tttttgtcac ccagcagctg ctggaatcgg tctacgccgc cggcgctgtc ccggcagaac
     3901 cgggcgaatt caccaaacgg gcgtttctcg ccggaaaaat gacactcacc caggcagagg
     3961 cggtcatgga tgtgatcggc gcagccggaa aacgggagct ttcctttgcc catgcccagc
     4021 agaacggcgc actctttcac cgggtgcagc agatctcaca gggcatcgtc agcagtctgg
     4081 gcgacctggc tgcctgggcg gactacccgg aggacgaagt cccggcagtc accccggagg
     4141 gactccacgc cgcactgcag cctgtcttgg aacagctgga cagcaccctt gccacctatg
     4201 actacgggcg cattctccag tcgggcgtgt cggcagtcat cgtgggcaaa cccaacgtgg
     4261 gaaagtccac cctgttcaac ctgctgtccg gcagtcagcg cagtattgtc actgaaattg
     4321 ccggcacgac acgtgacgtg gtggaggaac agatccgcct gggcaacgtg accctgcggc
     4381 tgtccgacac tgccggactg cgtgagaccg aggacgtgat cgaacagatg ggcgtgaaaa
     4441 tcgccgagga acgcctggcg caggcggatc tgattctggc ggtgtttgac accacccgtc
     4501 cgctggatgc agacgaccgt cgaatgctgg aacagctgcc ggacaagccg gtaatcgcca
     4561 ttctgaacaa gtccgaccag accgcctgtc tacatccgga aagcctggaa cagcagttct
     4621 cccgtgtcat caccatggcg gcaagggacg gcagcggtct ggaacagttg cgccaggcgg
     4681 tggaagagct gttctaccag gcggaagtga ctccggaact gggcatcgtg gcaaacgccc
     4741 ggcagaaaca gtgcctggaa cgggcgagag agcaggtaaa gcttgccctg gatgccctgg
     4801 atgccggcga actgctggac gctgtgaccg tactcctgga agaggcagcc gacgccctgc
     4861 tcaccctcac cggcgagcgt gtctccgatg ccgtggtgaa cgacgtgttc tcccggttct
     4921 gcgtgggaaa ataaaagcgg ttgtttcacg tgaaacattt tggattggat gatgggtaac
     4981 gggaaggcat acccttttgc tgaggtggga attttgcaag tgttaatata ggctccccta
     5041 acagaggagc caaatgaaag tctcctctca tagaggagat gcccgatagg gcagaggagt
     5101 attcagtccc ccttgaaaag gcgcccgaag ggtgtgcctt gtgctgggat gtcagcgtag
     5161 ctgacagggg gatttacaaa gagttactct tctttttttc tttgctaaaa agaaaaaaga
     5221 agcaaaaaaa gaaaagctta ccgcctaccc ttacttgaaa cggcagtgta tcacacgact
     5281 ggcagtgtga cttctgagtc atgcctgcaa cttttcaatt aaaagccttg aattggattt
     5341 ttcgaacaaa aatcccccgg aggggtaaca ggcagcttgt ctgccgtagc attgcctttt
     5401 ttgaacgttg aattggggcg aaaggtcagc ggcgacccgc cgctcgccta ctcttgtcct
     5461 gtgcggcaat gtatcacacg actggcagtg tgattatgat agatttacat ttatacgcat
     5521 ttacgcatta tataaaactg aggtgatacc tatggaatat tatcacatgg gaacttatga
     5581 tgtcgccgtg gtcggcgcag gccatgccgg cgtggaggct gctcttgcct ctgcccgtct
     5641 gggctgccgc acggtgctgt ttaccatttc tctcgaccag atcgccaata tgccctgcaa
     5701 tccgtccatc ggcggcacgg caaaaggcca tctcgtccgg gagatcgacg cattgggcgg
     5761 cgaaatggga aaggcggcag atgcctgctt tctccagagc cggatgctga accggggcaa
     5821 aggtccggct gtccacagcc ttcgggtgca ggcagaccgt gtccgctatc acaactacat
     5881 gaaacaggtc tgcgaaaaaa cgccgctcct ggagatcaaa caggcggaga tcgcccagat
     5941 ccagaccgaa aacgacagag tcaccggtgt ggtcacatcc ctgggcgcac agtatgccgt
     6001 ctcggctgtg gtcatcgcca ccggaacgta tctgaacggc agaattcacg tgggacaggt
     6061 ctcctatgag agcggtcccg atgcggcact gcccagccgt cccctgggaa aaaatctcca
     6121 ggaactggga ctgcggatgc gccggttcaa gacgggaacg ccctgccgtg tgcatcgccg
     6181 cagcatcgac tttgacgcca tggagcgcca ggacggcgac gaccatatcg tgccgttttc
     6241 cttcggctct gacccgtctc agatgcacaa ccaggtgtcc tgctacatca cctataccaa
     6301 cgaggagacc caccgcatca tccgggaaaa tctccaccgt tcgcccctgt tttccggaca
     6361 gatcgagggc atcggacctc gctactgccc gtccatcgag acgaagatcg tccgctttgc
     6421 cgacaaatcc cggcaccagc tcttcgtgga acccatgggg ctggaaacgg aagagtacta
     6481 cctccaggga atgtcctcgt ccctgccgga ggatgtccag ctggcatttc tccgtaccat
     6541 ccgtgggctg gaacacgtgg agatcatgcg cccggcttat gccatcgaat acgactgcgt
     6601 agaccccacg gaactccggg catccctgga gagcaagaag atcgccgggc tgttcggcgc
     6661 agggcagttc aacggcagct ccggctatga agaggctgcc gcccagggtc tggttgccgg
     6721 catcaatgcg gcgctggctg ttcagggtaa gccggcgttc gtcatggaca gagccacttc
     6781 ctatatcggc acactggtag acgatctggt gaccaagggc tgcgaagacc cgtaccgcat
     6841 gatgacatcc cggagcgaat accggctcat tctgcgccag gacaacgccg accagcgttt
     6901 gatgcccctg gggcatgagc tggggctggt tccggaggag cgctatcagc atatgctgga
     6961 gaaataccag ctggtggcgc aggagaaaaa gcgggtgcag aaaaccaatc ttgccccgtc
     7021 tccggaactg aacgcttttc tggaacagca cggcacatcc ggcatcacca ccggctgtaa
     7081 ggtggcagat ctgatccgcc ggccgcagct gggctatgac tgcattgccc cgttcgacac
     7141 cgcacgcccg gcactggatc cggtcatcgg ggagcaggtg gaaattcaga tcaagtatga
     7201 cggctatatc ccgaaacagc tggaacagat cgagcggatg cgcaagctgg aaaacaagca
     7261 gctgccggaa gatctggact ataccaccat tcgtggactg cggctggagg cagccgaaaa
     7321 actcaatgcc caccgtcccc agaatctggg acaggcgagc cgtatctccg gcgtgtctcc
     7381 ggcagatatt tccgtgctgg tcgtgtggga agcatccagg gagggtaacg catgatgaca
     7441 tatgagacgg caaaggcgct gttttccggc gcaggactga cgctgagcga gagccatttc
     7501 gcccagttcc agtgctatct gacagagctg gtggaagtca accggcacat gaatctgacg
     7561 gcgatcaccg aacccggcga ggtctgggaa aagcactttc tggacagcgc aattctgctg
     7621 caaaaggtga cactgcccct gggcgcatcc tgtattgacg taggaacggg cgcaggcttt
     7681 cccggcatgg tgctggcaat tctgagaccg gacttacaaa ttacattgct ggacagcctg
     7741 caaaagcgca tcggctttct ggaacagacg gcggcaaggc tgggactggc gaatgtgcgc
     7801 tgcattcacg ccagagccga ggacggcgca caggactcgg aactccgaga gcagttcgat
     7861 atggcaactg ccagagccgt ggcagccatg ccggtgctga cagaatactg tctgcccttc
     7921 gtaaagaacg gcggtatttt tgccgccatg aaaggaccgt ccgagacggc ggcgcaggcg
     7981 gaaaatgcgg caaagctgct gggcggagcg gtcgtcggcg aagaacagta caccctgccc
     8041 accgccggag accgccgcat catccgcatc gaaaaggtat ccgccacccc gaaaaaatac
     8101 cctcgccgca gcgataagat caagaaacag ccgctggtgt gagttgacat aacggaaatg
     8161 ggtaacgcat cgtag
//
LOCUS       DAWENO010000089         8126 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3635_length_8126_cov_10.811919, whole genome shotgun sequence.
ACCESSION   DAWENO010000089 DAWENO010000000
VERSION     DAWENO010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_8126_cov_10.811919"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6481..6490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1824 a         2222 c         2287 g         1783 t
ORIGIN      
        1 gactatcggt tcgatatcgc caagcaggcg ctggtcaagg acagagagaa tacccagtac
       61 atcgcctgtg aattttccgg ggatgtctcc aagctgcgtc tgaagtttac cggaaaaaat
      121 gacggggacg gggtagtcct gcagagcgta cagctgaatg cgcccattcc ctttgccgtt
      181 tcgccgctcc ggatggcgct ggtgctggtg ctgggaacgc tggcttatgc ggtggcgtgc
      241 agcactacgc tgaaacgccc gtatctggag acgaaaaagc tctgccgccg taccattaca
      301 gtgatgaccg cagcggtcat ggccgtggca gtgctgatcg ttatggcaaa gctgcccgac
      361 ggcggtttcg ccggacagtt caagctggac agcggcaacc agatcacaca ggaacaggtg
      421 gacgctttcg aaaacggaca aatttccctg ctgaaagaag tggacgatgc gctgctccag
      481 gtggagaacc cttatgactg gggaatgcga aacgactccg gcgcaaaggc ggagtgggac
      541 cacgtattct atgacggcaa gtattattcc tactacggca tcgcaccggt ggtgctgctg
      601 tatctgccgt atcacaagct gaccggacac tactgctcca cgaacctgtc tatcctgctg
      661 ttcagcctgg tgggactgct gttcctggca atggcatatc tggcattcgt caagcgctgg
      721 tgcaaaaacg tctctgccgg aaacatcatc gccggcatgg cgatcctgtt cagctcctgc
      781 ggcatctggt acagcgtggg acgcacgctg ttctatgaga tttccatttc ctccggattc
      841 gcctttgtgg cactgggcgc ttatttcctg ttcagttcca acgtgctgag cgagggcaag
      901 gtgtctctgg tgcggacggc gctggcatcc ctgttcctgg gcatcgccgt gctgtgccgt
      961 ccgactctgg cggtctatgc cgtggtggca gtgctgtatt tcctgtacgc catccccaag
     1021 tccgggaatg tgctggtgca ggcagaggac ggcacttcgt ctctggcagt ccgcaagccc
     1081 cggcgcatcg cctacgtgct gtgcgcagcc ctgccgctgc tggcgctggg catcacccag
     1141 atggtgtaca actatgcccg gttcggctcg ccgctggact tcggcatcca gtattcgctg
     1201 accatcaacg acttcaccca ttcccagtac cacacctcgt tcgtgctgat cggtctgtgg
     1261 aattatctct tcgccccgcc tcagttcctg ccggagtatc cgtacatcag tacgcctttc
     1321 tccaagctgg acaccaacgg cttttacttc aacgatgacg gcaacacctc cggcatcctg
     1381 ttcctggcga tcccggttgc ggcatatctg cttgcccgtg cggcactgcg ccgtctgccg
     1441 gacaccaaga cacgctggaa atacggcgtg atggtgggac tgccctgcgt ggtcatgccg
     1501 ctggtcatca tctgctcgat ctgggaaagc ggctatgcgg tgcgctatac ggcggacttc
     1561 tcctgggaga ttctgctggg cgcactgacg atcctgttct tcctgtatca gaagtccagg
     1621 aacgaaacga aaaaggatct gacccggaag tttatggcgg ctgcgatgct ctgcgctgtt
     1681 gtggtaaacg gcgtgcagat cttcaagttc gcattccccc aggatcagta cccggcgatc
     1741 tgtgaccatc tgacacagct gatcgcattc tggaagtaag gagcatgaat ctgatggata
     1801 cattatattt ggtcattccg tgttataacg aagaagaagt gctgccggaa acttcaaaac
     1861 agcttctggc gaaaatacag tcgctgatgg cggcggagaa ggtctcggag aagagccgca
     1921 tcgtctttgt caacgacggc tcgaaggacc gcacctggga gatcatccag gcgctgcacc
     1981 aggagaatcc catttaccag ggcgtgtgcc tgtcccggaa ccggggacac cagaatgcgc
     2041 tgctgggcgg cttgatgaca gtgaagaatc actgcgacat ggcaatttcc ctggatgccg
     2101 acctccagga cgatatcgat gccattgacg agatggtgga caagtactat gccggaaatg
     2161 acgtggtcta cggcgtgcgg aatgcccgga aaacggatac ctttttcaag aagttcaccg
     2221 ccgagggctt ttataaggtc atgaagatga tgggcgcaga cgtggtgttc aaccacgccg
     2281 actatcgtct gatgagccgc cgtgcgctgg acggactgga atccttcaag gaggtcaata
     2341 tgttcctccg tggcgttgtt cccatgatcg gctataagag cgacaaggtg tactatgccc
     2401 gaaaggagcg tttcgccgga acttccaagt atccgctgaa aaagatgctg tcctttgcct
     2461 gggaaggcat cacatccctt tccacaaagc ccatcgccct cattacccgg ctgggtacgc
     2521 tgatcttcct ggtgagcatc atcatgctga tctattcctt cgtgcggcac tgcatgggcg
     2581 caaccattac gggctggact tcgctgatcg tgtccatctg ggcaatcggc ggcttgcagc
     2641 tcctcgccat cggcatcatc gggcagtaca tcggaaaggt ctacttagag acaaaggaac
     2701 gcccgaagtt tatcattgaa acgtatctct ccgatgagga cgcagatgcg gataaggata
     2761 agacacagaa gtaattaaaa gtaacactgc ataaaaataa atcctcggag acttgttccg
     2821 gggattcttt tggagggttt ggttatggca tatatttcca acgaaaaccg ttattctcag
     2881 atggaatacc gccgctgcgg aaacagcggg ctgaaactgc ccctgctgtc cctggggctg
     2941 tggcacaact tcggcgacac caatagcctg gagcggatgg cacagctctg tgagacggcg
     3001 tttgacggag gcatcaccca tttcgacctg gcgaacaact acgggccgcc tgctggtgcg
     3061 gcggagcgaa acttcggcag actgctgaaa accattttcg ccggtcaccg ggatgaactg
     3121 gtcatcagca ccaaagccgg ctatgatatg tgggacggtc cctacggcga cgggggcagc
     3181 cggaagtatc tgctggcgag cctggatcag agtctgaaac ggctgggtgt ggactatgtg
     3241 gacatcttct accaccatcg ccccgacccg gaaacgcctc tggaggagac catgggcgca
     3301 ctggcgcagg cggtgcggag cggcaaggcg ctgtatgtgg ggctgtccaa ctatgacggc
     3361 gagaccatgc gccgggcggc ggacatcctg gaacagctgc actgtccgta catcatcaac
     3421 cagaaccggt actccatcct agaccgggag acggagcgca acggcatcct ggatgccagc
     3481 gccgaccgga aaaagggcgt tatcatttac agtcccctgg cgcagggaat gctgaccgac
     3541 cgctatctcc acggcattcc ggaggacagc cgcatccgca ccgacggcag atttttgcag
     3601 gcatcccagc tgacggaaga acgcctggcg cagctccggc agctgcaggc gatcgcacag
     3661 gacaggggac agacgctggc acagctggcg ctctgctgga tcctgcggca gaaggcggtg
     3721 accagcgtgc tggtgggcgc atcccgtccg gaacagatca cggacaccct gcagatcgtg
     3781 gggcagaaac cgctgaccga tgaggaactc cgggcagtcg atgcagcagt aggactgtga
     3841 gaaccggcgt gtgcggcttt ttcaaaaggg gcttgacaat cccgaaaaaa tagcgtataa
     3901 tagggataga gagagggcat accagtagac ggtctgctcc cactgcttga gttaaagatt
     3961 taaccgccac aagtttggag actacgggcg gttatttctt tttcttgccg tggtgattct
     4021 gattactatt tttatgatta tagtaaatgt acaagatcac gctgatacaa tccagcagaa
     4081 agctgccgat tttcagcaga tcaccaaagg tgacgtattg atccatcata ccgatcacct
     4141 cccatctgtt caagtgagca gtgggagcaa aaatcagaaa aagacttgac cgcctaccgt
     4201 ttcaggtatg ccctcagata ttcagtatac cacgttttgc aaaaaatgtc aaacaaaaaa
     4261 tccggcagca ggactcagat tcctgttgtc ggatttttgc atttccatgt aatttacgct
     4321 tgacttatgc ttttcttatg cctggtttca gcatttgctt gtccttatgt ttaatgactt
     4381 ccatcataat aagcgccatg atgacagagc agatgtccgc aatgggctgg gcgaagatca
     4441 gaccgtccag accgatcaga gcgttggcga tgaacagtac cggcaggaaa atgaatccct
     4501 ggcggctgac ggacaggatc agagatgcca gaccgtgtcc catgccctgg aaagcgttgt
     4561 tgttcacgaa aatgataccc aggaacggtc cggaaaccat cagcgcccgg agcatcttca
     4621 cggcgtagtt ctgctgggtg ataacatctg ccgcagagcc ggaagagccg gaggtcataa
     4681 agatgctgat gaccggctca gtgaacagga agtacagcgc cgtcagaatc agccccagca
     4741 ccagggtgca gaccatgctg aaccgtacca cttttgtcat cttggtgaag ttccgtgcgc
     4801 cgtagtggaa tcccaccagc ggctggatgc cgataccgat gcccagctgg atgaacacta
     4861 ccagcatatt tgctttcatg gcgatgccca tgccgccggt ggcgagaacg tcgatgttat
     4921 tcagcagccg gttcagcacc aggttggaca ggctcatcag cacattagtg aaaaacatgg
     4981 ggacgccgat ggagaacacg ccgctgaaaa tgccgccttt catggtgaag tgcttcggat
     5041 tcggggagag catggtgcgg ctgactttca gatagatgag gtagaacagc gttgccaccg
     5101 tgttgccgat gctggtggcg atggctgcac ccttgacgcc catgcccatg gcaaggatca
     5161 tgatggggtc caggatgatg ttggtcacag cgccgatcat catgccgaac atggagactt
     5221 tcgccgcacc ctcgcccttg accaggttgc tcatggcgct gctcagcacc gtggggattg
     5281 cacccacgcc gatgtagaac agatagtccc tggtgtactg ttccagattc tgatagttct
     5341 gcaccagctt gtcataggcg tcgccggaaa tatccttgga aatattggcg atgttggcgg
     5401 acgcatctgc acccaccagc ggcaggatcc agctcacgcc cagggtcagc gccagggtgg
     5461 cgagcagtcc cacaaagaca gcgccccaga aactgaagct gctgattttt ttcacccgtt
     5521 catattcccg tgcgcccaga gaacgggaga taaaggcgca gccgccgatg ccgaacaggt
     5581 tgccgaatgc catgaggatc ataaacaccg gggtcgccat ggaaacggca ttcatctgat
     5641 aggggtcgtg ggtctgtcct acaaagactg tgtccaccat gttgtacagc acggtgacca
     5701 gcatactcag caccgacggg atcgccagta ctgccacagc tttggggata gactcgatct
     5761 caaaaacgga ggttttcttt tctttcattt gcatataggc ataacttcct ttctgttacg
     5821 gcgaaaaacg aggcagaaat gcggtgacat tctgcctcgt ttgcatttgt tcggttgagg
     5881 tactcttata cgttaaagcg gaacagcacg atgtcgccgt cctgcatcac atattccttg
     5941 ccttccagac gcaccagtcc cttttccttg gcagccgcca tagagccgca cgccatcaga
     6001 tcgtcatagg agacaacttc ggcacggatg aatccctttt cgaaatcggt gtggatcttt
     6061 ccggcagcct ggggcgcttt cgttcccctg gtgatggtcc atgcacggac ttcctgcttg
     6121 ccggcggtca gataggagat gagacccagc agagaatagc cctcccggat gatgcggttc
     6181 agaccggatt cctccagtcc cagatcttcc aggaacattg ccttgtcgtc tgcctccatg
     6241 tctgcgatat cctcttcgat cttcgcacag atgggcagta ctgcggcgtg ttctgcctca
     6301 gcaatctgct tgacttcctg gtagtgttca tttttgtcca cgccgttgcg gaagtcctct
     6361 tcgctcatgt ttgcggcata gatcaccggc tttgcggaca gcagcggcat atcgtggatc
     6421 cagctcagct cgtcctcggt gaagtcaaag ccacgggcag acttgccctc ttccaggtgc
     6481 nnnnnnnnnn tcgcccttca gcgccttttt ggtgcggtcg atgcggcgtg ccaccatctc
     6541 catatcggag aagatcagct ccaggttgat ggtctcgatg tccgatgccg gattgattct
     6601 gccgtctaca tggatgatgt tatcgtcctc aaagcagcgt accacgtgga cgatggcgtc
     6661 cacttcccgg atgtcgccca ggaacttgtt gcccagacct tcgcccttgg atgcgccttt
     6721 taccagtccg gcgatgtcca cgaactccag ggtagccggg gtgaacttgt ccggctcgta
     6781 catttcagcc agcttgtcca gacgtgcgtc cggcacagag acgatgccta catttttttc
     6841 aatggtgcag aacggatagt ttgccgactc tgcgcctgca tttgtcaggg cgttgaacag
     6901 ggtgcttttg cccacgttgg gcagcccaac catacctaat ttcattgtaa cagctccacc
     6961 tttttgaaga gcgtgcgcag catccggcat atgcagagag atgcgccgca ggctctgaga
     7021 ttatccggca ttgcgccggg gattgacata tcattgacac atacattata gtttagcaca
     7081 gaaagccgga tttgtcaaga tttttgcgaa aatgcgctcg agaatactct gcacaaaagt
     7141 gtaaaccatc cgtccaatcg taggggcgaa cattgttcgc tcgttccggt atcagagatt
     7201 gtctattatg tcccccttga aaaggcgccc gtagggtgtg ccttgtgctg ggatgtcccg
     7261 aagggacagg gggattaagt ttgcggttca ctatttcagc acaaatcgca tccccaaaaa
     7321 acccccttgc aatctcttcc gaaatttgct atactattat aacagcgata cattcaagca
     7381 aaatcgaaac ggagtgaatc tatattgaat atcccacaga aactggctga cgagttccag
     7441 ctgcggcagg aacagatcga caacaccatc gccctgctgg atgacggcaa gaccatcccc
     7501 tttattgcac gctaccggaa agagcggacc ggctcgctgg atgaccaggt gctgcgtgcc
     7561 atcgacaacc ggctgcaata cctgcgcaag ctggaacagc gcaagagcga gatcctttcc
     7621 gccatcgagt cccagggcaa gctgaccccg gaactgacgg aaaagataca gaaagcggag
     7681 actctggtgg agacggagga tctgtacctg ccgtatcgtc ccaagcgaaa gacccgtgcg
     7741 tctatggcga tcgcccgtgg cttggagccg ctggcacgga cgctgatggc gcaggatgcc
     7801 ggaacagacc cggtgcaggc ggcacaggcg ttcctcacgg aagaagtgcc ggatacggat
     7861 gcggcgctcc agggcgcaat ggacatcctg gcagaggaga tggcgaacga cgccgacctg
     7921 cgcaaacaga tgcgccgtct ggtgatgctc accggtacga tacagtccca cgctgtggat
     7981 gcggatgcgg aaacacccta tcagaactac tacgactatg ccgaaccggt aaagcgcatc
     8041 gtgggtcacc gtgtcctcgc catcgaccgt ggcgaacggg agggcgcact gaaagtggca
     8101 gttacgctgc cggagggaca cggggc
//
LOCUS       DAWENO010000090         7975 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3728_length_7975_cov_10.467803, whole genome shotgun sequence.
ACCESSION   DAWENO010000090 DAWENO010000000
VERSION     DAWENO010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_7975_cov_10.467803"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6170..6179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7556..7565
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2016 a         2164 c         1854 g         1921 t
ORIGIN      
        1 ggtttttccg gttccggcat ggccggcgag tgcaatgttc ttgattgtca tggtgatttc
       61 gctcctatct tcattttttc tatttcatct gccaaattgt cggcagtaca gcaagttgca
      121 tagttttatt tcaaccgcat aatacaatta tagcacattt cacattacaa tgcaatagcc
      181 ttttgcaata aattgaatat tttcggaata ttgcatagag acaagctgat atttttacgc
      241 aaagcggcat ctcgccttgt gaatatttca caaactttca aaaagccatt gacaatgatg
      301 tgaaaaaatg ctatgatatc tcttaatttc aaaacctgcc gtggaaatac ggcaaacgaa
      361 ccaaaggagt acagcacatg gacgccgccg acagcgacac ggacattatg acaagtgcat
      421 acatatatta tggtatgaga atccatatgg cagaactgcg ccgtttccag aaatggctgt
      481 gtgtcagtgc tgcccggaaa aggcatggtt ttcgccggat gcggtgttag ctgtgtcttt
      541 ttgcatacgt atatacttat aataattttg tcctaaaatg aaaggttgga gaatccataa
      601 attatgatcg gtctaatttt actgcaaatc gctttgattg cactgaacgc catattcgcc
      661 ggcacagaaa tagcggtgat ctcggtaaac gaaaccaaat tggaaaagga agtggaaagc
      721 ggaaacaagc gtgccggatg gctgctgaaa atggtgcgga actcctccgg atttctcgcc
      781 accatccagg ttgctattac cctttccgga tttctgggcg ctgcattcgc atcagacagt
      841 ttcgccgaaa agctgacctg gctgctgcaa aaaacaggca tcggcatcag tgaatctgtt
      901 ctgaacacca tttccgtcat tctggtaacg ctcatcatct cctatttttc cattgtgttc
      961 ggcgagatga tcccgaaacg catcgccatg aaaaatgcgg aacgcttttc cctcaccatg
     1021 gctggtccta tcacagtcat tgcaaagctg tttgcaccgc tggtatggct gctcaccaag
     1081 tccacaaacg gcgtgctgcg gctgtgcggc atcgacccca acagcgacga ggaaaccgta
     1141 acggaagagg acctgctgct catggcggat gccggtcaca aaaagggaac cattgatgcc
     1201 acggaaaatg acatcattaa aaatgtattt gcctttgacg atctcacggt aggcgagatc
     1261 tgcacccacc gaaaagaagt agcggtcctg tggatggacg aaaccattgc acagtgggaa
     1321 cagaccattc acgaatcccg ccattccgtc tacccggtgt gcggcgacag tgtggatcag
     1381 atcgtaggcg tgctcaatgc aaaggacttc ttccgtctga aagaccgcac cagagatttt
     1441 gtcatggaac acgctgtcca cacaccctat ttcattcacg aaaacatgaa ggcagacgaa
     1501 cttttcagca aaatgaaacg acacgccgat cattttgctg tggtagtcga tgaatacggc
     1561 ggaatgtgcg gcatcatcac cgtgactgac ctggtagagc agctggtggg tgattttgac
     1621 gatgacgaga cggagcgtca ggaaccggaa ctggaacgac tggactcccg gacgtggaaa
     1681 atccgtggca cctgctccat ccacgatgtg acaaaggcgc tggaacatga gttcacggac
     1741 gcagaagagt ttgagacatt cagcggcttt ctcattggca agctgggcga gattcccaag
     1801 gacggcagcc ggctgaaact ggagatcgaa ggctatctca tccgcattct caacgtgcgg
     1861 cagcattgtg tggaaaaggc aatcgtccgg gtagcggata ctgctccggc ggcggaatcc
     1921 taacgccatt cttatccaat taaattcccg atcaaatcgg cacaggcaga cagcgcttgt
     1981 gccgattttt ctgttttcga ccccatctta tattttacgc agattttaca tagccgccgt
     2041 ttagacttta cacagaacga ttataataag actgttctta tccgtatgtc cggacgagga
     2101 cagtttccaa gaaatcggtt tccgctgcgg aaaccagaaa taaggagtcg aaacttatgg
     2161 atatcttttc cgttatcacc ctgctgggcg gtctggcgtt tttcctgtat ggtatgaacg
     2221 tcatgtcctc cggtctggaa aaactcgccg gcggcaagct ggagatcatt ctgaagaaga
     2281 tgacctccaa caagttcaag agcctgctgc tgggcatggg catcaccatt gccatccagt
     2341 cctcttcggc gctgaccgtt atgctggtcg gtctggtaaa ctccggcatc atggagctga
     2401 gtcagaccat cggcgtcctc atgggttcca acatcggtac gacactgact gcatggatcc
     2461 tcagcctttc cggcatcgag ggggacaacc cgttcctgag aatgctgaaa ccggagtcct
     2521 tctcccccat cattgccctg atcggtgtca tcatgattat gacagcaaag cacaaccgca
     2581 ccaaggatat cggcaagatc atggtgggat tctccgtgct gatgaccggt atgacgctga
     2641 tgagcggcgc tgtcagcccg ctggcagatt ctccgcagtt ctcgcacatt ctcactgcat
     2701 tccgcaaccc catcatgggc gttctggtcg gcgcagtctt taccggcgta attcagagtt
     2761 ctgccgcatc tgtgggtatc ttacaggcgc tgtctctgac gggacaggtc acttatggca
     2821 tggcgatccc cattatcatg ggacagaaca tcggtacctg tgtcactgcc ctgctctcca
     2881 gcatcggcgt aaaccgcagc gcaaaacgtg ttgcctgcgt ccacatctgc tttaactgca
     2941 tcggtacact gattattctg ccggtgttct acggtctgta ctggctgctg gactttgcct
     3001 ttgtggcatc ttccatcgac ccggcaggcg tggcactggt acacaccatc ttcaacattg
     3061 ttaccactgc aatgctgctg cctttcacca agctgctgga aaagctggcg tatacactgg
     3121 tacgggacag caaaaaggaa aaagaggcga aagaaaagca cgctatgctg gacgagcgcc
     3181 tgctggcaac cccggctgtt gccattgaag tatgtcacgg cgtgaccatc gaaatgggcg
     3241 aactctccaa gaaaaccatg gagcttgcca ttgaaatgct gttccagtat gacaaggata
     3301 ttgaagaaca aatcgaggaa aacgaaaacc gcatcgacaa atacgaggac aaactgaacg
     3361 cttatctagt acgcatcagc aagcacagca tctcctccaa ggacaaccgg actgtatcca
     3421 agatgctcca ctgcatcggc aacttcgagc gcatcggcga ccatgcggtc aacattatgg
     3481 aatcggcgca cgaactgcac gaaaagggac tgcacttctc cggcgacgcc gccaaggagc
     3541 tgcgcaccct gtgcgacgct ctgctggaaa cgctggatct ggcgttccag gcatttgaaa
     3601 aggacgacct tgccattgcc caccaggtcg agccgctgga agaagtcatc gacacactga
     3661 acctggaact gaaaaaccgc cacatcaagc ggctgcaaaa cgaagaatgc accgtggaac
     3721 tgggctatat ttaccaagat ctgctgacaa acatcgaacg catttccgac cactgctcca
     3781 atatcgccgg tgttctcatt gagatcgacg aaaagcagaa tatccataag tatctgtata
     3841 aactgaaaga aaccgatgag acctttcagg aatcctatca cgaatatctg aaccactact
     3901 atctggagct gggtcagccc tctctggacg aagtgatcga cgcataacaa gcaatagaaa
     3961 aagccctctc ggactactcg tctgagaggg cttttctgca ttccggaaca gcgtggagaa
     4021 gtacctcccg ttcattgggc agcttatcgt ccagcacctg ttccagcagc atatgcagaa
     4081 tctttccgat ttgtttcccc tggggaatgc ccagtgcaag aagatcagcg ccgcctacgg
     4141 caagatcttt tacacgcagg caggcgtttt ctgccagcaa ctgttccagg cagttttccg
     4201 ccgcagtcca ctgtgcttca cgccgttcca gcacgccgtc tgcctttccg gcatcatctg
     4261 cccgtttcag cgcaagcagg cggcgtacct gtgtctcacc aagctttgcc aggagacggc
     4321 gcatgaaacg tggactgtcg ctgagcggca tatcatgatg tgccaccagc gtacagacat
     4381 cgtggatgcg tgtatggtca gcgtgcagcc gccgcaggat ctgacgggac atctcagcgc
     4441 ttttctcagc gtgtccgtaa aagtggtcga tgccgtccgc tccggtaaaa cggcaggcag
     4501 gctttcccac atcgtgtagc agtgcagacc agcgcaggat gatatcacgt ggcgcatttt
     4561 ccagcacgtg cagcgtgtgt tcccacacgt catattggtg gtacgggttg tgctggggac
     4621 actgctgcat cggcaggaac tccgggaaaa tcgtgttcca tacggtggga tactgccgca
     4681 ggatttctgc cgccgcatcg ccgtccgcca acagcagttt ctgaaattcg atgtcgatcc
     4741 gctccaccgc aatgcgcccc agcagcggtg caagccgcac tgccgcagca gctgtttcca
     4801 gttccagtgc aaggcgatag caagcggcaa agcgcagccc acggagaatc cgcagggcat
     4861 cttctgaaaa gcggcgttcc gggtctccca cacaccgcag cacgccggca gccagatcat
     4921 ccctgccgcc gcagagatct accaacccac gctccgggtg atatgccata gcgttcatgg
     4981 taaagtcacg ccgccgcaga tcctcttcca ggctgcggac aaaggtcacc tcttccggtc
     5041 tgcggcagtc tgtatatgtg ccgtctaccc gataggtggt gatctcataa ggctcaccgt
     5101 tccacagcac tgtgatcgtg ccgtgttttt ccccgatggt cagcacagta cagtccggaa
     5161 aacaggcctt tgtctcccag ggcatggcgc tggtacacaa atcccagtcg tgaggcgttc
     5221 tgccgcagat catatcccgg acacaaccgc ccacggcata cgcctcatac ccggcagact
     5281 ccagccggga gatcagctga gagacagatg ccggcagaga aaatgccatg aaaatcacct
     5341 cgcttttgtt taaaaacgaa aactgcattt acaggcttat aagatatatt ccagctgtct
     5401 ttcctttgtt ttcatcccca ttttctcata gaacttttct gcggatatgt ttcctgccca
     5461 cacgttcaag gtcacttcat agcatcccat tctctttgcc tcctgcttga catagtcaaa
     5521 caggctttct ccgatatgct gtcctctcgc aaaacgatct acgcagagat catcaataaa
     5581 cagggactga aactgtacca tgttgtttga aaaaggctgc tctttcacct ggcaaaatgc
     5641 gtatcccatg caaacatcct cttcatttac tgcaacataa atgggctttt tctcatcttt
     5701 caaaagtgct gctaactctt gttccgtgta ttttgtagta tccggaataa aaatatccgg
     5761 tctgatctct gcatggatct gaaggatctg tcccaacaat tcgataattc ggggaatgtc
     5821 tttttcctgt gcttttctga tagtcatttt tattttctcc tattctaatt tggattgata
     5881 ttgttttaca aactggaatt tagcatatca attccacttt gatcttctcg cagtagattt
     5941 caaattctat atcttcttct aaagaggata tacggtatcc acaatccttt tcatatcccc
     6001 aatcagaaca atcctctatt gttagtccgc tgaaaaagcc atcatccata tcgaaactga
     6061 ttgcaccgct aacattgaaa agggataatt ttattgcata cttttcttgt acatcccgca
     6121 atatcaattc tatacagcag atataatcgc agtattcttc actatacatn nnnnnnnnnt
     6181 ttccttgtaa taatgcaatt ctgaaatatt accccatcta tgccagtctt gtaattcgtc
     6241 aggcagcttt ttctgaagaa tatcaattgc ttctatatta tccgtatgaa acattatcca
     6301 cctctaactt ccgatttatc atggcttttt tacctatcat acattatact acacctctcc
     6361 ggaaaaagca agaaacctca tccccggctg cgaaaatagt gatacttctt ccggttggca
     6421 atcagcagta ccactgtcac aactacgccc aggaactctg ccacaatgat cgaactccat
     6481 acgccgttca gctccaggaa cagcggcagc accaacacgc agatgatctg gaacaccagc
     6541 gtccgtgcaa acgagatcac cgccgacacc agtccattat tcagcgccgt aaagaatgcc
     6601 gaaccgaaaa tgctaaagcc gcagattaga taggacaggc tgtagatccg gaacccttga
     6661 cgtgtcattt ccagcagccc ggcatcatag ctgacaaaga tgttcgccag cagagatgcc
     6721 atcagttctc cggctgccgt caaaagcaat gcgcctacag acaaaatgcc gatgctgcgg
     6781 cgcagcagac cctgtagctc ctcgtgatgc tttgccccgt aatgataccc tacaataggc
     6841 gcactgccga tggaatagcc catgtaaatg gcaatgaaaa tgaagttgac gtacatgatt
     6901 accccatacg cagccacgcc gtcctcaccg gcaaaacgca tcagctggag attgtacagt
     6961 gtattcacaa tggacatgga caggttcgtc atcatttccg acgcaccgtt ggagcaactg
     7021 cgccacagcg gatgccattc cagctttgtc cgcaccagtt tcaaccggct ggtgttggga
     7081 cgtgcaaagt agatcaacgg cacgacaccg ccgatagtct ctccgattcc ggtcgccaca
     7141 gctgcgccgg cgagtcccca atggaacaca acgatgaaca ggaaatccag caccatattc
     7201 gccacgcctg ccgccaggat cacatacagt cccagatgcg gtttttcggc ggtgataaaa
     7261 aagctctgaa acatattctg gagcataaac ataggcagcg tgaaaatcaa gatccgggcg
     7321 tacagcacgc agtattccag catctcgccc cgtgcgccca tcatcgatgc aatgggtcga
     7381 atgaggaaaa agcacaccag cgccgccgca aatccgatga taactgccgc atagatcacc
     7441 atggaaaaat accggttcgc ccgtttggca tcgccctcgc cgcaggtctt tgccaccaga
     7501 gcgctgccgc cggaacccag cataaagcca atcgcaccca ccgccatcag cagcgnnnnn
     7561 nnnnnaagtg aagatcatca ttatgataga cggaaaaaca aaccgcagca gttttcctat
     7621 ggtaaaatga tctgaaatct gtatccgcat tatttttctc ctttttcccg aaattccggc
     7681 gccagatgtg ccgtttgttt ttgcagcagg tgaatgaatt ttcccgtcag cgccaccagc
     7741 tgctcccgtt ctgcctctgc catttccgca aacgccgcct gctcggcatc gtaaatcggt
     7801 ttcacgcttt cgtcagcatt tcttcgaccg gacgctgtca gatagatatg cttttctctg
     7861 ccctttccag actccagccg cagcagtccc tgcttttcca tctttttcag tgcagagttg
     7921 atggtctgct ttcggctcag cgccagtgcg cagatgtccc gctggcggca gccgt
//
LOCUS       DAWENO010000091         7888 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3764_length_7888_cov_12.395251, whole genome shotgun sequence.
ACCESSION   DAWENO010000091 DAWENO010000000
VERSION     DAWENO010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_7888_cov_12.395251"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2496..2595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3693..3702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1544 a         2219 c         2169 g         1846 t
ORIGIN      
        1 gcacgaagtg ccggagggat aatccccctg ccctagcggg catccccctt ttcaaggggg
       61 actttatcgc cactcaccat gttccccttt gaaaaggcgc ccgcagggtg tgccttgtgc
      121 tgggatgtca gcgtaagctg acagggggat tcaatgatta gaaacgtccc tgtaggggcg
      181 aacatggttt gcccgttggt gtgtcactcc tccggcactt cgtgccacct cctctatacg
      241 aggaggcttt ctcaaatcgt ctcctcttta gaggagatgc ccgacagggc agaagagtca
      301 gcccggtcga acgatgttcg cccgttggta cgttaccgga atatttcggg tcgatgtggg
      361 catcgacccc tacaccatcc tatgaaacga ttccaaatac atttgtaggg atagatgccg
      421 taccctgtgt tttacttcga ctccagcgca gaccactctg tgatctcacc ggtgtaaacc
      481 tgcttgatct gatccttggt gatagcgtcc aggctgttgt ccttgttgac gatcacagcg
      541 atgccgtcca ttgccagaac agtactcttt acgccggtct cagtgtcttt cagttcacgg
      601 gatgccatgc cgatatcgca gacgccctcc tgggtggaag tcacgccggt ggtggagtcg
      661 ctctgctgta cgtctacgct gacatccggg tttatgtcgg cataagcctc tttcagcttg
      721 gtcatgaccg gtgtgacgga agaagaaccg gctaccacaa cgctgccctt tgcgccgttg
      781 gaagtgtagc tttctgtacc ctgtgccaca tagccctctt tgctgataac gtcagcggca
      841 tcggtactca tgatatagtt gaagaaatcc tgtgcagcgt cgctcagctc ctcgccgtct
      901 ctggtgacca ggttgaacgg acgggagatg gaatagctgc cgtctgccac cgtgtccacg
      961 ctgggtgcta cgccgtctac ttccagcgcc ttgacggaat cattcagcga accaacggaa
     1021 acatagccga tggatgcagc attgccggct actgtggtca gcataactgc ggtgctgttg
     1081 gtgacctcag cgctggagac ggtgttgtcc ttgtcgtctg ccacgatgcc gcacagctcg
     1141 gtgaatgcac ctcttgtgcc ggagccgtcc tcacgggtga ttacggagat tgcgccggat
     1201 gcaccgccgc cgcatccggt gagactgctt gccattacgc ccagtgcagc gactgctgct
     1261 gcgatctttg tcatcttttt cataatagat actccatttc cggacaccat ggtgtccctt
     1321 cgccggttcg ttgtgaaccg tgtttcgctt tttttatcct tgttccaatg aacaatgtta
     1381 ttataccaag cctatgtaaa gtctgttatc gcagcaatgt gaaatctggg ttaaatgtta
     1441 actcctcggc gtgtgccttt gttaactccg gaaatatcac gcagtttcgg tgcttttcgg
     1501 attgttaaca aagaaaaata cgttatgtta aacgaaaagg gattgatttt ctgcacattt
     1561 tgtgttataa tttccgggaa ggcggctggg tggtgtgtaa agcgaaccag tagggctaaa
     1621 tgactcctcc gccctgtcgg gcacctcctc tatgagagga ggcatatagg ctccccttta
     1681 gggcgcccgt agggtgtgcc ttgtgctgag ctgtcaccgt aggtgactga ggggttatcc
     1741 ttttagagga gctgctgact gaggagtcac catatcaccc accgccgcca aatcaccaga
     1801 aaggacagat ccctatgaat tttacggaac tcgcaaaaaa acgctgctcc gtccgtgcct
     1861 atgcagacaa gcccattgcg ccggagcatc tccaggcagt cctggaggca ggcagactgg
     1921 cgcccaccgg caagaaccac cagccccagc gcatcctggt cgtacagtcc ccggagggtc
     1981 tggcgaagat ggcacaggcg atgcacaaca ccaacgccta tacagcaaag gcagtcctca
     2041 tgatctgctc tgacaccacg gactgctggg tgcgcagctt tgacagccac tgcatccacg
     2101 agatcgatgc gtctatcgtc accacgtata tgatgctcgc cgcaaccgaa cagggtctgg
     2161 gttccctgtg ggtggagcgc tttgatccgg agatcctgcg gaaggaattc cacctgccgg
     2221 acaactacgt gccggatgcc ctgctctgcc tgggctatcc ccagccggag gcagtgaagt
     2281 ccccggagcg ctatgacacc gagcgcaagc cactggagga aacggtctgg tacgagagtt
     2341 atcaggcgtg atctgaaaaa ttgcagcaca accaggctgt cccataagcg gggcagcctt
     2401 tttttgttgt gcggtcttat gatgtttgtc cgttggggca actcctccgc cctgtcgggc
     2461 acctcctcta tgagaggagg catatatagg ctcccnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnntgtca ccgtaggtga ctgaggggtt atccttttag aagagctggc
     2641 agccgtaagg ctgactgagg agtcattccc tacggtagga acacgccgcc attttccaca
     2701 cccatttccg cagctcaaca aggattgcag ataaaggacg catagaccga ggacggtgtt
     2761 tccggggcga caggctttgt cgtcggcgct ggttccggcg tgttttcgcc ggaactgcct
     2821 gccttttttc gttcccaggt acgcactgcc gctttccagc tgcgcatagg gttctgtccc
     2881 atacgccaac cgttcgcctc gtagtagtcg tagaactgtt ccgggtcgat gccgttctgc
     2941 cgttccgcac agtatgcggc gatctcttcc ggttcgggtt tcacccactg cgccgcctta
     3001 tccagccgct ttccggaaaa attccctttc cctgcctgcg cagccgtcgt tgtccttgtt
     3061 tccggagcgc cgtctctctc tggatataac aatgactctg tatctgcatc tgactctttt
     3121 tctggctctg tctctgactc taactctgac tctaactctg actctaactc tttctctatc
     3181 tctgtatcta caatgtgatc cgtctccggt aacgatgtga tcacattgtg atttggctcc
     3241 ggtaacattg tgatcacatt gtgattcgat gactgaactt caatttcacg gtcagaatct
     3301 tccgaatggt tctccttttc tgtctttgga gtccgatatt tccgcatgat tgcagcacgt
     3361 tccgtctccg accccgtcat atcttccatc tgcggcatga acagcgcacc gttttcctgc
     3421 acctcgacca gcccggcgct tgttagcagt gccatggcgg tttcggcagc cggcagcggc
     3481 gttcccgtga tctccgccag ctttttggta tcatagggaa tctccatctt ccccacccgg
     3541 cgcatgagta cgccgtccga ccgcagagac cgcaggcaga gtttcagata aaagaggatg
     3601 tacactgcgc cgttttcctg ttcttccagc cagtttatct cgtctccctc gaaaaaatcg
     3661 ttcttgagtt tcagcgaata ataccgttta ttnnnnnnnn nngtgcgttt tttcccgaga
     3721 gaaaaaatgt ccctctataa ataggcgctc agaggggaaa agttgcacgc cggcgtgcaa
     3781 ctatgaacaa gaatttacac gatatccaaa gaaaatttcc gtttctggga gttctctgga
     3841 aaatccggcg catttcccgg catcggacac ccggcatccc acggaacaaa accggcggtt
     3901 tttcatatga tgtggtgtgg tgaaatgatt ctgtaagggt gaacattgtt cgcccggtgg
     3961 tgcgtcactc ctccggcact tcgtgccacc tcctctaaag aggaggcttt ctcaaatcgt
     4021 ctcctcttta gaggagatgc ccgacagggc agaggagtca gcccgggcga atgatgttcg
     4081 cacctacgat gggacgggga ttttgcacat tttgtaaggt ttccgttttg tgatacttct
     4141 gtagggacag atgcccacat ctgtccgctg taacaagttg cccattccat acacccgaat
     4201 caatgtgtga aacaactctg taggggcgaa cattgttctc ccggtggtgt gtcactcctc
     4261 cggcacttcg tgccacctcc tctaaagagg aggctttctc aaatcgtctc ctctttagag
     4321 gagatgcccg acagggcaga ggagtcagcc cgggcgaatg atgttcgcac ctacaatgga
     4381 aaacctttcc cttacccaca tacccacaac caaaaggagg aacgaccatg cctttgataa
     4441 gtctctgtat aatcgcacgc aacgaggaat ccgtgctggg acgatgtctg gattcggtgg
     4501 cggatctggt ggatgagatc atcctggtgg acaccggctc gaccgaccgc accaaggcgg
     4561 ctgcggcgga atatgcggcg aagatctacg attttccctg gtgcgacgac ttttcggcag
     4621 cccggaactt cgccgtctcc caggcggtgg gcgactattg gatgtggctg gacgctgacg
     4681 atgtgatcga gggcgagaac cacgagaaac tccggcgcat cctggagcat cccgacgcag
     4741 acgtggtctt tctcccctat tggctcagct ttgacgaatc cggagcgccc cagatggtct
     4801 cccagcggga gcggatcttc cggcgcagcg gcggctatca gtttaccggg gctgtccatg
     4861 aggcagtcgt tccacacggc aggctgcgct acggcgatgc ggcagtctcc caccggaaac
     4921 tgcgccccgg cgaccccgac cggaatctcc acatctacca gcaaaagctg cggcgtggcg
     4981 agtcgttttc cccacgggag cagtattact acgccagaga actttgcgac cacggggcat
     5041 accgggctgc gctgccggtt ctggagcgct ttctggaaga gggcagcggc tgggcgccgg
     5101 actgcatcgg agcgtgtctg ctctgcgccg gctgttaccg ccggctgggg catccggaca
     5161 ctgcccggga acggctgttc cggtcatttc gctacggcat tccccagccg gaggtgtgct
     5221 gtgagatcgg cagcattttt ctggaaaagg gtcagtatca gacggcggcg ttctggtatc
     5281 atctcgccat ggaaacggga caccagcccc accagggatt ccgccagcag gcgtgctgcg
     5341 actatctccc ggcgatccag ctgtgcgtgt gctacgaccg gatgggtgac atttccaccg
     5401 ccgccgcata taatgaactt gccgggagct ttcgccacgg cgacaaggcg gtggcataca
     5461 accggcagta ttttgcggag aagatgggga aaacgggcgg ctgaaagagt gtgtcttgtg
     5521 ctgggatgac acgaagtgcc gaatggataa tccccctgcc ctagcgggca tccccctttt
     5581 caagggggac tgtatcgtct cctctcttag aggagatgcc cggcagggca gaggagtcaa
     5641 cccggacgaa caaggttcag acagatgccg cctgccttct cttacagagg agtcgccgcc
     5701 tttccccgtc cctctcccac gatcaaagaa aggagcgata ccacatggca tattgcaatt
     5761 gcggaaatcc aaatcccctg gatccggagc gctgctgcat tcctgcgccg ccgcccatgc
     5821 cctgctgctt acagggaccg cccggcgtga ctggtccggt gggtgcaacc ggtccggctg
     5881 gtgctgttgg tgcgactggc gcaaccggcg ctacgggtgc aactggcgct acgggtgcaa
     5941 ccggtgctga cggcgctgtt ggcgcaactg gtccgactgg tgcaaccggc gctgacggtg
     6001 ctgttggtgc gactggtcct accggtgcaa ctggcgctac gggcgctgac ggggctgttg
     6061 gcgctaccgg tgcaactggt gcgacaggcg ctgacggggc tgttggtgca actggtccta
     6121 ccggcgcaac tggtgctacg ggtgcgactg gtgctacggg tgcgactggt cctgccggga
     6181 cggtgacacc ggctgacccc atagcagatg ctcagagcct ggaggacatc gtggagcagt
     6241 tcaacgcact gctggcgaac ctgcgggctg ccgggctgct gcgtaactga gccaaataca
     6301 aaagggctgt cccaaaaaca gggcagccct tttcttgttg tgttttgtta ctcctcagtc
     6361 aagcctatgg cttgccagct cgtactgagc ggtccccttt gccctttggg catctcccca
     6421 ccccgtgggg agtcacctct aaaagaggag cctatataca cccacttgcc tcctctgtta
     6481 gaggaggtgt ccgcaggacg gaggagtcaa cccgggcgaa cattgttcgc ccctacggta
     6541 ggacggggat tttgcacatt ttgtatggtt ttcgttttgt aatacttctg tagggtcaga
     6601 tgcccttttc cacagcgtcc tctcactctc ccgaaagcgg cttgcgcagg aggtcgtccc
     6661 ggatcttcca cagctgttca ttgtcggcac ggtcacgcat ggtgacgaaa tagagcgagc
     6721 cgtacatggg cagagtgtcc acataaatgc accccaaccg gttttcgtcc cgatcctgct
     6781 ggatggtggg cttcagcaca aaggcgatgc cacgcccctg gcgcagcagc tctttcagca
     6841 cgataaagga atccacctcg ataacttccc ggaaatcgta cagggaaatg ccgctgcggc
     6901 tgagatggcg ctgtaccagt gtccggatgc cgcagccagt ctccagcaca atgagcggct
     6961 gtttcagcag cgttccccag gaacagccgt acagctgttc ggacagttcc gggcttgcgg
     7021 cagcgatgat gttttcccgg cagagttccg tcgagtccac cagcatatcc ggcagttcgc
     7081 cttccatgat gatggcgtcc agcattccgc tctgcatccg tttcagcaac cgccgggtat
     7141 tcccaacgtg gatctggata tgcggctcgc tctcccggtt ggcataggca cagagccggg
     7201 gcagcgcaaa ggaaaacgta gcggacggcg taatgccgat ctcaaacgcc gccgtctcct
     7261 ggagcgtctg gagacgcttt cggatctcca cattctgcgc acgctggaga atgaccttgt
     7321 cccggagata ctgccctgcc tcggtcattt ccacacgttt tccgtcatag tggaacagcg
     7381 gacactggta gaaatgctcc aggtacttga tgtgctgggt cacagacggc tgggtcagat
     7441 ggagatgctc cgccgttttg gtataacttt gtgtttcaca cagggacaaa aacgtgtcca
     7501 ttcgataatc taacataaag ctgcacctcg ctgcagtgtg ggttgggtgg ggttgcttgt
     7561 tggtgtgtta ctcctccggc acttcgtgcc acctcctcta taagaggagg cttttacaaa
     7621 tcgtctcctc tcctagagga gatgcccggc agggcagagg agtcaacccg ggcgaacgat
     7681 gttcgcccct actgtgggac ggggattctg catatctgcg gtttccgttt tgtggtactt
     7741 ttgtaggggc gaacagtgtt cgcccgttgg tgtgttactc ctcagtcagc cttacggctg
     7801 ccagctcgta ctgagcggtc ccctttgccc tttgggcatc tccccacccc gtggggagtc
     7861 acctctaaaa gaggagccta tatacacc
//
LOCUS       DAWENO010000092         7803 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3825_length_7803_cov_9.569437, whole genome shotgun sequence.
ACCESSION   DAWENO010000092 DAWENO010000000
VERSION     DAWENO010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_7803_cov_9.569437"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1109..1118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2794..2803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3963..3972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1937 a         1774 c         1807 g         2255 t
ORIGIN      
        1 gcggagaaag tggtcatttt gcacaaatgt gttcgttcct ctgtgaaaaa tataaaatat
       61 atgaaatttt atatatgatt caaagaaagt gaaaacaatc gcatttgctt gctttttctg
      121 gatgccagaa aaatgggatt tcaatttcgt ttctgatttt ttgcttgttt tttatgtggt
      181 tttccataaa aaatgtgccg aaaatcgtcg aaattccgtg caaacgtcat ttttctgcga
      241 tgttcttgac aaatgaaaaa aaatagtata taattcgtag tagattatga ttttggagga
      301 acatttgaca tgacaaaaac tgatttgatc gaaaagctgt acgaagaaaa ttactgcaca
      361 aaaaaagatg cagaggctct ggtaaatgct gtatttgatg caatccgtga agcaatggtt
      421 gctggcgata aggttaccat ttccaagttt ggtacttttg aagtgcgtga gcgcagtgca
      481 aaggaatgca agaatcctcg tactggcgaa ccggttcagg ttccggcttc taaggctccg
      541 gtattcaagg caagcaaaaa cctgaaagaa tctgtaaacg cataagatgc cgggaggaat
      601 gagcaatggc aagaccgaag aaaactgaaa gcgccaaaac aacggtaacg gaagcaacaa
      661 caaaaaaagt caccgctgct gctccgaaag caacacgagt tcgaaagact gtcagtgtta
      721 gggtgcggta tggcggaatt gcgtgggcag cagagcatag tcagaagaaa acggcagcaa
      781 aggatattaa gttgtacgta aagccggaag aaaacatggt gtattatgta atcaacgggg
      841 attccggttc attcgcactg taaaatttca agatagatat tttgaaagca cttgtatttt
      901 tacaagtgct tttttgtgtt atacgggata aacgaaaaag ggtagagaca aatctgtctc
      961 tacccttttc atagttcctt attcgaatgt actcttacga aacagccatc tgaatgtaat
     1021 cgctgcagtc gctgccgaca gatgcgttaa caacttccaa tgcgcctgca tcatacttga
     1081 aacggaaaat cattgcaacg atgccaggnn nnnnnnnntg ctgcaattgt gaatgttccg
     1141 gattcagcag agcctgcact cttttccgat gcagaaccga cagcaatgtc accgtccaca
     1201 aatgcgaccg gtacactttc agcgtcccag tttgcaaaat catttgcacc gcttgtgatt
     1261 gcatagttgc cgtccggagc gttgtctttg atcttgaaag taacatccag gaagtcaccg
     1321 ttgcagacaa cgtcttctcc gttggacata tccagccagt atgcctggaa tgtctttata
     1381 tcagactgat agctgctgcc gcctgaggtg tttccgccgg aagtagaact ttcattcttg
     1441 ctgctgtttc cgcccttcac aacttctgtt accacatttc cgttgtcatc ctttacaggc
     1501 tgaccggaat catcagtaac gacaacataa gagtcttccc cggaagaccc gctattctga
     1561 gatgtaacac ccttatttac tggaacatct gaatttcctt cggaactatc gtctccatcc
     1621 aaagcgtatt caaacgtcat atcttgtttc tgcatttttg ccagatcttc cgaactcaaa
     1681 gaatcttcag ccttttcgtt ggaactatca ccagccgctg caaaactgct gtcattgttt
     1741 ttttctttgg aactaccatc ctgtgacttg ccgcacgcag tcagcgacac cccaatcgca
     1801 caagccaaca aacaggcaat ccatcttgct cgagttgatt tcatagaaca agttcctttc
     1861 taccagaggt taggcgcatc aaaagaaaca cactgcgtgt tcttgatacg atacgtctta
     1921 ttatacacgc ttttctgtga aaagtcaagc ccttttgtaa aaatgtcata aagacgtttc
     1981 aaaaaggctt gatttttttg aaaaataggg gtatacttgt atgtattggc aagatatgcg
     2041 ctgacatttt tgctgtgtgt agggatgcga tgtgaaagga gctgtttcac atgaaaaacg
     2101 aaaagcagaa gaatacaaat gagaagaggc tttctcggaa aacaattatt tcacttgttg
     2161 cagtggtact ggtttttgct gctgtcagtg ttgctatgat tcctttgatc cgtttattgt
     2221 ccagtgaaca tggacaggag atcattattg aaaaaatgca gagttttggg atatttgcgc
     2281 cgctgctgtt tgtcctgctg caggtgctgc aggttgtggt ggcagttatt cccggcggcc
     2341 ctgttcctat tatcggagga atccttttcg ggcagtgggg cgggttgttc ctgtcgcttg
     2401 ccggtttctt tttgggtacg gttttggttt attatctggt gcaatggatc ggcagaccgc
     2461 tggtagaccg ttttgtaccg gaaagtcact tcaagaaatt cgattttctt ctggagggta
     2521 agcggacaga acttttgatt tttcttgtgt ttttacttcc ggggctgcca aaggatgccc
     2581 tcacctatct ggtctctttt aatcccagaa tcaaaccact ctatctgttt attttgacta
     2641 cgctgggcag aacacctgct accgtgctga ctgtttttat ggggaaaagc ctctgggaag
     2701 gaaatttcac ccttgcaatt gtgcttgcgg caattattgt tgttcttgcg gttgccggga
     2761 tctttatcaa gcgaaaagtg gacaagcgtg cagnnnnnnn nnntataggc tgttttgtta
     2821 tttaatgcgt aacggttgca aaaatagaaa ggaaattgct tatgcagcat aacgacttac
     2881 aggatgctct tgcgtatttg cgtacgcttt tttctacata cgaatcgcaa ggggttcaaa
     2941 ttacgggcag cagaatgcct tttacacagt atgcgaattt cgctgcaatg aaatatctgc
     3001 ttttcctggc aaatcaggat cgtgtgatta cggatacgga aacagagttt atcaatgtct
     3061 gcctgggaca gtctgtaaca acagcagcta tggaacgctt tttgcagcag ttttccatct
     3121 ccttttccgg tgcagcagat gccttgcttg ctgtgttagc ggcgttggtg cgtgttgatc
     3181 tgcaaggaaa cgggacgaat ggaagtgtat ccctcttgtg gatgaatacg gtggctcaga
     3241 tgggaaaaca gttttctttg gacactgcag gaaaaatcga accgcagcag cagatcgttc
     3301 aaaattcaat gatgcagcag ctttcacgct atcggagtgc agcgatttgc agtgcgcatc
     3361 gtgcagaact ggatgtccct gcggaagttc ccagaaattt cgggcaggat atgccgtctg
     3421 tgccgcagca gaaagaaagc gttgtctctg gggcagaagt tacagaggcg ccgacagaaa
     3481 cgctggagga gcttttggca cagctgcacc gattggtggg actgcagaat gtgaaggaag
     3541 aattggagag tctgattcac ctgctaaaaa tccgctctat ccggcaggaa cgagggcttc
     3601 ccctgcccca gacttcactg catatggtct tttccggaaa ccctggaaca ggaaaaacca
     3661 cagttgcccg gctgttggca aagatctatg cccgtctcgg tgtgctgaaa aaaggacatt
     3721 tggtagaggc agaccgtgca actttggtca gcggctatgt gggacagacg gcaattaaaa
     3781 cgaaaaaggt aattgaaaag gcaatgggag gcgtactgtt cattgatgag gcgtatacgc
     3841 ttaccagttc tggtgggagc aatgacttcg gcacagagac tgtgaatacg ctgctgaaag
     3901 aaatggaaga tcatcgggat gatttaattg tgattgttgc tggatatccg gaagaaatgg
     3961 aannnnnnnn nnatggaacg gtttctggac accaatcctg gactgcgttc ccgtttccga
     4021 cagatcatct tttttgcaga ttacaaaccg gaagagctga tgttgatttt ctccaatatt
     4081 tgtgacgatt attgtttgaa aatgccgccg gaaacctacc attatgttca ggcgtacttt
     4141 cgaaaaaggt gtgcggaggt gggaaaaggg ttcgcaaacg ccagagatgt gcggaacttc
     4201 tttgaatttg cactgaccaa tcaagccgac cggctggcaa agctgccgga gccgctttct
     4261 gatgaaactc tgctgacgct gctgccggag gacgtacaga gcatcccagc acagtcttag
     4321 catgggcgtt tacataaaga tagatacacg aaaaaggtcc gtcagaccaa gtacattgta
     4381 ccggtctgac ggaccttttt atggaaggag atatattgca ttctcccgtt tggtttattt
     4441 gcagatcatc tgttttccgt cataatcaat ttcgattaca gtgccgggtg caggatcatc
     4501 tgcgatgatt ttctttgcga ccaaggtctc aattttagac tgaatcaacc gtttcagcgg
     4561 acgtgcgccg tagttgacgt cataaccctg tgcaacgata tattcttttg cagcttgtgt
     4621 tacgtgcagt tccagctgct tgtcttccag acgtttccgc agctttttca aaagcagatc
     4681 tacaatactg aagatctcct tctgatttaa tggcttgtag aatacgattt catccaaacg
     4741 gttcaggaac tctggacgga actgctgttt cagcagtgcg tccacttctt ttctggcatc
     4801 ttcagaaata tttccgtttg catccatgcc gtccagaata atcggagagc caaggtttga
     4861 agtcaagata ataatggtgt ttttgaagtc aactgttctg ccctgcgaat cggtgattct
     4921 gccgtcatcc agtacttgca gcaggatgtt aaagacatcc ggatgtgctt tttccacttc
     4981 gtccaagagg acaacggaat agggtttccg gcgaactgcc tcggtcagct gaccgccttc
     5041 ttcgtagcca acatatccgg gaggcgctcc gatcagacgg ctcacgctga atttctccat
     5101 gtattcgctc atgtcgatac gaacaatatt gttctcatcg tcgaacagcg cctctgcaag
     5161 tgcttttgcc agttctgttt tgccgacacc ggtcggaccg aggaacagga acgaacccag
     5221 cggacggttc gggtcctgga ttccggcacg ggaccgcagg attgcctcac tgactttttc
     5281 gactgcctca tcttgaccga tgacacgttt gtgaagaact tcgtccatgt gcaggagctt
     5341 ttcacgttcg ccttccatca gttttgtaac cggaataccg gtccatctgc cgacgatacg
     5401 ggcaatctct tcttctgtga ctttatcccg gagcagagag ttcttgcttt ccttttgttt
     5461 ttctgcaatg gtctcctctt cttccagctg ctttttcagc tgcggcagac gaccgtattt
     5521 taattcagca gctttgttca gatcatagtc acgttctgca cgttcgatct gtgcattgac
     5581 ctgttccagt tcttcccgca gtttctgtac tttggaaatc gcatcttttt cattttccca
     5641 tttggctttc atttcgttga actggtcacg catctgggac agttcctgct gtacatcttt
     5701 caaatgcgcc tgggagagtt tatcatcctc tttctttaat gctgcctctt caatttcatg
     5761 ctgcatgatc tttctgcgga tctcgtccaa ctcagtgggc atagaatcca tttctgtacg
     5821 gatcattgca caagcctcat ctaccaggtc gattgcctta tccggcagga aacggtctgt
     5881 gatataccga ttcgacaagg tcgcagctgc aatcaatgcc tgatcctgga tctttacgcc
     5941 atggaatacc tcgtagcgtt cttttaagcc acgaagtatc gaaatggtat cttctacagt
     6001 cggctcatca accaggacgg gctggaatcg acgttccagg gcagcgtcct tttcaatata
     6061 ctgccgatac tcgttcagcg tggttgcacc gatgcagtgc agttcaccac gtgccagcat
     6121 cggtttcaga aggttgccgg catccattgc accgtcagat ttacctgcgc cgacaatggt
     6181 gtgcagctca tcaatgaaca ggatgatgcc gccttcactc tttttgactt cgttcaagac
     6241 agctttcaag cgctcttcga attcgccacg gtattttgcg ccggcgatca atgcgcccag
     6301 atccagagaa aagatttttc ggtctttcag attatccggg acatcaccac gaacgatacg
     6361 cagtgccagc ccttctgcaa tagcggtttt acctacacca ggttcaccga ttaaaacagg
     6421 gttatttttt gtctttctgg aaagaatacg aatcacattt cggatttcat tgtctcgtcc
     6481 gattaccgga tccagtttgc cggcacgagc ccgctctacc agatcttgtc cgtatttgtt
     6541 cagaacgtca taggtctctt ccggattctg atcggtaaca cgctgattgc cacgaatttt
     6601 catcaggact tccaggaatg cctcttttgt gacagaaaac ggctgtaaaa cctttttcaa
     6661 atcgtttccc attgcgtcga acaagccaag catcagatgt tccactgata caaagtcgtc
     6721 tttcatgtgg gcagcctctt tttctgcctc ccgcaatgca cggtcaacat cctgtgaaac
     6781 gtatacttca tctgctttgc gtccacttcc ggtaactgcc gggagattgt cgatcgcatt
     6841 tttcagctgt atctgaacgt tatcgacaga tacacctatc ttctcgaaca gctgcggaat
     6901 caaaccgccc tcctgcccta aaagtgcgct aagcaggtgt acctgttcga tctgctggtt
     6961 ttggttctca attgccagac catttgcgga ctggatcgcc tctaaagact tttgtgtcaa
     7021 tttctgtgtg ttcatgagaa ctccctccta ttttcattgt tgtttcccat tcgttttgtt
     7081 tgttttttct atggctttgg gagcagcttt tcatgcagag cgacatactg cttttccagc
     7141 gcatgacgca ggattgcggc tggtacgcct tgctgcaatc cctgaaaata ggactgcaat
     7201 actgtgtgaa acagctgaat atacttgccg actgctgtcc ccagtgcctc atttgatgaa
     7261 atgccgtgac gacgcagccg ccgtgtcaag cgattggatt cgatttgata cagttcctgt
     7321 atctctatgc cttcgtatgc ctgttccaac tgtacccaaa acaaaaagaa ttgttgaatt
     7381 gcattcagca aagtttgact ttgtgtgcgg caggagattt tcagaatgcc aaccttttcg
     7441 ccgtgcattt cacgatgcag ttccacgctg tagcggatgg tcggacgata cttgtactgt
     7501 acagaaccaa gaattgaaag catcgcatcg gagccttgct cctggatgcg aaatgcggaa
     7561 ttcatctgct gtgtcagaca agcaaaaatc tgtcgcatct gctgctccgg cgttacgcag
     7621 gaaacgtgtt ccgccagaat ctgattgatg agatttgaac gactggttcc ctgctgtgct
     7681 gcaaggcggt caatcgcagc taccacatca tccatgagaa tcaagctgta tgtgcttcgt
     7741 ttcatctgct cacctccttt ttctttctga ttctattata tcacaaagaa ataatttgtc
     7801 aag
//
LOCUS       DAWENO010000093         7620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3956_length_7620_cov_9.915268, whole genome shotgun sequence.
ACCESSION   DAWENO010000093 DAWENO010000000
VERSION     DAWENO010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_7620_cov_9.915268"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    686..695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1148..1157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1666 a         2193 c         1911 g         1830 t
ORIGIN      
        1 tcaggtgcta gtcctcgcaa gggggtggag gttcaagtcc tcttatccgc accaacagct
       61 ttctctgaaa ttttgaattg catccccgac tctttccctt tcgggtgttt ccatcgggta
      121 caaattggaa acaactgctg cggatatggc ggaattggca gacgcgctag cttcaggtgc
      181 tagtcctcgc aagggggtgg aggttcaagt cctcttatcc gcaccaacag ctttctctga
      241 aattttgaat tgcatccccg actctttccc tttcgggtgt ttccatcggg tacaaattgg
      301 aaacaactgc tgcggatatg gcggaattgg cagacgcgct agcttcaggt gctagtcctc
      361 gcaagggggt ggaggttcaa gtcctcttat ccgcaccaag gattctggaa agctgactat
      421 gtgaaagcat ggtcagcttt tttgtttttc tcatgctgcc ggaggtgtct ttatgcagga
      481 gatccgcaaa accattgacc gcaccgtatt cggactgatc ttatacacgc tgctgcggta
      541 cgccgtcttt gccgcagaac cgctggcacg aaagtgctgg gtctggctga cgataccgga
      601 cagcagccgg cggcaggaag tgctgacaca gctgaacagc agcgcatccg gcagcggtgt
      661 ttccagcatt gccgctgtat gtgtgnnnnn nnnnngtgtg ctgtttctcg gctggtactt
      721 ccgaaaaagc acaccgcccg gaaagctctt ttcccaaaga cagcccctgc cgctgaaagg
      781 atttctgctg ctgagctgca ttttcatggg agcgcagctg atctattccc tgttcgggaa
      841 cagcctggaa ctgctgctcc ggctgtgcgg atataccgcc atgggacagt tggatgctgc
      901 atcggcgcaa agcactaccc tttccatgtt cctgtacacc gccgtttttg cgccggtcgg
      961 cgaagaactc atctaccgtg gactggtgat gccgcagttt cagcgatacg gaaaggtgtt
     1021 tgccattgtg acctctgccc tgctgttcgg cgtcatgcac gccaatctgt ttcagatgcc
     1081 gtttgccttt ctcaccggac tggtgcttgg ctacacggcg gcggaatttt cgctccgctg
     1141 gtccattnnn nnnnnnntcc attctgctgc acctgctcaa caacctggta ttcggtgaac
     1201 tgctgggcag ggtgatgggc tatctttccc ctgcggcaca ggattatctc tatcgtggca
     1261 tcaacatttt ctttttcgct ttcgccgcat tctggctgct ccgccaccgg gaacagctga
     1321 aacaatatct ccgggaaaac ggatgcgccg gaaagactta cgccgccgct tttacggcag
     1381 cggcaatgct ggtgtttctc atagggaatg tggggctggc ggtttccggg gttcagaagt
     1441 tgggataaaa aaatatcgca ccatacagaa cagcctgcat ggtgcgatac tttttattgc
     1501 tgtacaggag aagcggtgtt cctgcacgca ttatttttca atgccgtctt tcaaggtatg
     1561 gtcagaccag ttatccagcg tcgttttcac cggatatgcg ccgcagtatg tcccatccac
     1621 gctgaggatc acagtgcatt tctgctgtcc ggatgacgct gtgtacactt cgaaatcaac
     1681 agtctggtaa agatcaaacc gtgacacatc catataatct gccaggtctc cacaatcccg
     1741 gagtgtatat gtcccgtcct gcatcggcgt tcctgcatct tccatcttcg tcaatgcctt
     1801 ctcaattgcc gtgccgcact tttctgcctc aatgttattt gccgtgatct ttccggcatc
     1861 atccagtgca aggtacaaca cgctaacacc ggaattcaac atcgcatcag tacaatacgt
     1921 gggatatgtg gaataggtca gcgttccgtc tgtttctttt ccatagcata caacagacag
     1981 cgccgttcca tccgcagtgt aaattgcaat atcctcataa tttttcagaa aatcgcattt
     2041 atcgtccagt tccttgtcaa tttttgtgtc attcccatag tgatacattc cggtgtcaga
     2101 aagccccatg gacttaaaaa cggcattcgc cgcctcatac actgctttgg catcatcctg
     2161 ttccaacgcc tttttctcgg catctgtttc ggaagattct tctcccgaag ttgtcacagc
     2221 attttctgtg atccgctttc cgctgcgctc agaaacatcg gttttggatt gtccacggaa
     2281 aacagccagc agaattacac agaccgatgc cgccagaacg atgcaggtca aaagaatcca
     2341 catcgtcttt ttcggtttct tttgcattgt tcccgaaaaa ggcatctgcg cagactcttc
     2401 cgcacatctc gccccacact ctttgcaaaa cttcgagtca tccggaatct tcgcaccgca
     2461 actcttacaa aacatacaag caacctcctc cgttcggata aaaccccggt gtgacacgcc
     2521 gctttacggc gtgaaatccc ttttattgta catgatattg atgtgcaatc attttataaa
     2581 ttatagataa atattaaatt atacagtttt atccatttca gattcctttt tattcctgtg
     2641 taagatagtc cacagattcc agatcgggca gcttaaaaag cagtctcagt actggaacac
     2701 actatcccct gcaaactccc gtaccagcga atctgccggt acgtgttcca gcgtcagcgg
     2761 tgccacatca tcccacatct gccgcagttc cagcagcatg gggtgctgca tttccgcctc
     2821 cagttccccg tccagcagcg tcttgtaaat gccaagctca tagggcagga acgtgtctat
     2881 ggaaaaatca gcacgctttt tatagggtgc aatatatttg tgttcccctg cctgcacgtg
     2941 ttcaaacatg gagagcgtct ctgccacact gcgctggcgg aagttcttat cccggagcat
     3001 ccggcgcatg agccgcagtc tggacggatg cagcacagtt tctccgtttg tcacacgagt
     3061 gcggacgctg acatagagcc gcacggtcat ctcctcgggg atggtgatga catccggatt
     3121 cagtgcatgg atgccctcca gcaaaagcag ttcgccgggt ttccgggtca gttgccagtc
     3181 ctggtcgata cgggtcgtgg tgggaaaatg ataccggggc atccaggtcg gcttgcaagc
     3241 aatgatgctt tgcagctgct ggttcagata gggaatgtct acccgtgccg gagactccag
     3301 atccagcttt cccgttgccg ccatttcccg ctgctgcggt gtcatggtgg aaaagaaatt
     3361 gtccatggaa atggtgtggg tctctgcgcc ccagccatcc agaaagcgct ccagcagaaa
     3421 agccgtggtg gtctttcccg aaccggacgg ccctgcaagc agaatcaccg gacgctccac
     3481 cgccgttttg cggatggcat ctgccacctt ttccagttcg tccagaaacg ccagatctgc
     3541 ctgccgcatg gcatcctcga aggcagtcat agcttggttg atgtctgtca gttttctgta
     3601 tttcatggtc gtttcacctt tctgcaatac caaatcacac tgcacgcata caaatgcaga
     3661 tgcgctattg gtattatagc atatttccac agtttttgtc aattgaaata gtatgatgtt
     3721 gtacactttt ccgaattctg tagtgcagga ttccggagaa ttcctcacac cgcatttcga
     3781 aaagaaagca aatccccgga aacgaaaaag aaaatcagtg gacaacacag cggttttgtg
     3841 gtataatggt agcatcacaa tgaaaaagga gcgatcacaa atgcgtagcg atctgatcta
     3901 tattccggac agcgaactgg atgccctcat tcagcaggac accccttacc tggatctgac
     3961 gactctgggg ctggacatcg gcgatgagcc gggttcgctg tcgttctata cccgtgaggc
     4021 gtgtgtcctc tgctgcaccg aagaggtatc ccgtattctg acgaagctgg gactttccac
     4081 cgcatattct ctccccaccg gcacacagat cggcgccggc gagctgttct tcaccgcaga
     4141 gggcagcgtc tgcgacctgc accgggcgtg gaagatctgc cagtccctgc tgtcccactg
     4201 ttccggtgtt gccacaaagg caagaaatat gctgagccag gtgcaggcga tctcgcctgc
     4261 catctctctg acagcggcac gggcgcattt tccgggaaca aaagcactgg tcacaaaggc
     4321 agtcgttgcc ggcggcgtca ttccgcaccg catgggactc tcggaaactg ttctcatcta
     4381 tgagacccac atcgcctgca tgggcggtct ggaagtgttc ctggaacgct atccccagca
     4441 gaaaagccac ttctgcgaaa agaaagtctt tgtggaaaca cagtccgcag agacggcgct
     4501 ccgctgtctc tccgtgggcg tggatgccat ccagtttttc cagatgcagc cggatgcgct
     4561 ggcaaaggct gtggaacagg tcaaggcggc atatccccac tgcaccatca tcgctgccgg
     4621 aaatctcacg gaagaaaacg tggcagaata tgcggcgacc ggcgtagacg gtctggtcag
     4681 ctcggcattc taccgggcag aacccattca gatgcaggca caatttcaca ccaactaaag
     4741 gagtattttt gacatgaaaa ccatcggaat catcggcgca atgccgtcag aactcgtaga
     4801 catccgcctg ggtctccccg attcggagat ccagaaaaag gctggtttcg actactatat
     4861 caaccaggtg ggtgacaccc gtatcgtcca cgtctgcagc ggcatcgcaa aggtcaacgc
     4921 agcagtctgc acccaggcgc tcatcgacaa tttcgcaccg gacgctgtca tcaatgccgg
     4981 cattgcaggc ggcatgaaca acgacattca cgtttgtgat gttgtagttt ccagcgaagt
     5041 gctgccccac gacctggatc tgcactttct gaaggactat ccgccttact gcggcatcta
     5101 caccgcagac caggcactca ttgacctggc agtcaagacc tgcggttctt tcgaagtgag
     5161 atcctttgtg ggacgcatcg tctccggcga ggcgttcgtc acagacagcg ccgtcaaggc
     5221 ggagatccag cagcggctca cgccctatgc cattgacatg gaaagcgccg ctgtgggaca
     5281 gtgcgcctac cggaacagcg ttccctttgt cagcatccgc tgcatctccg acaatgcgga
     5341 tgacgaaggc gctatgtcct tcgatcagtt cgaaaagatc gccgcaaaac gtgtggcaga
     5401 catcgtgctg cgcatgatcg aatcgctctg agggaaaacg ctatgacatt acagcagctg
     5461 caatatgtga tcgccattgt ggaacacggc tcgatcagcg aaacggcaaa acagctctat
     5521 gtggcgcagc ccacgctttc cagtgctgtc cgcaccctgg aagaagaatt tggcattatc
     5581 attttccacc gctccgccag aggaattgcc ctcaccgagg acggacggga gttcctgtcc
     5641 tacgccagac aggtggtgga acaggcagac ctgctccacc agcactacgg cgcagccaag
     5701 cagacacgcc agcggtgttc catttccacc cagcactatg cctttgcggt ggacgctttt
     5761 gtccggctca tccagcggct gtccccggag tcctacgagt tcaccctccg ggagacacgc
     5821 acccacgaga tcatggagga tgtgcgcaac ttcgacagcg agatcggcat tctgtacctc
     5881 agcagcttta acgaaaaggt gatccgtgcc atgctggagg aataccacct ggaattccac
     5941 tccctggtga acgcagaacc gcacgtcttt ctccgggcag agcatcctct cgccggacgg
     6001 gcatctgtca cgaccgccga cctggaacca tacccgtttc tctgctttga acagggtgtc
     6061 cacaactcgt tctactttgc ggaggaactg ctcagcacca tgccccacaa aaagtgcatc
     6121 cgtgtcagcg accgtgccac cttgttcaat ctgctccgag gcgtcaacgg ctacaccatt
     6181 tgcagcggca tggtaagcgc cgatctgaac ggcggcgaca ttgtctctgt tcccctggat
     6241 acgccggagc ggatggagat tggctggatc tccaatccga gaatccagga gagccacggc
     6301 gctcagctgt atatccagga attgcagcag gtgctgatgg agtatcagca gccggtgagg
     6361 aaataggaca cgacaaccga tatttcagag cctgtcttga caggacacgt gtgacaggtt
     6421 cttggattcc ggtttttggc agcatcgtac aagctttgta cggtgctgcc tttttgtttt
     6481 tcccaccaaa ctgaccggaa acgcatcttc tcccgattgc ccgtcctgcc gccgcagatt
     6541 catgcacatt gttctaaaat gatgcaagag attctgacag atatttttct ctccaaacgg
     6601 tataatgaat acaccaatga tatttcaggc atctcgcctc agagcctgcc ggcgtatccg
     6661 aatgtacgca gactgcaacc acggattttc tcgaactatc ccgaaaaatc cgcagacaga
     6721 acacgtgcag aaaggtacgc cggcagacgc tccgcaaaga aaggacgttt ccatcatgca
     6781 caaccccagc caacctcagc tcctccatcg catccccgtt ccacgcattc tccgggagac
     6841 cggcggcggt tttctctccg gactgctcct tgccggtaca tacatgggaa ccatgacctc
     6901 gccgctgccc atcgccatcg ccgccaacct cagcgcaccc ggtgccattg ccgttctcat
     6961 cggcagtctg atctcctata cgctctccaa taccatgctg gacaacctgc ccctgctgtt
     7021 tgccctggta gtcgtggtgt gcctgcgcct gttgaaacgg cctgcaaaaa cagccgccgg
     7081 catggcgtgc agcaccggca tctgtgtgtt tctctccggc attgccgtct ccctggtatt
     7141 ccacgccagc ggcgcagaag tgatcggcta caccatgact gcggcactca ccggctgtgc
     7201 ctcctatttt atgcacgccg tcttttccag cgtccgcagc acaggcaaga ttcccctgcg
     7261 ctccaccgac ggatgcgccg ccgcagtcgt tctcattctg gcggtggcag cattcagctg
     7321 ttacggcatc ccgtccatga acgccggctg cgtcatcagc accgcagtca cactaatcgg
     7381 ggcaaagaag ttccggtgtg ccggcggcgt aatctgcggc gcactgacag cctgcggcgc
     7441 aatcctgggc gcaccggatg ccggacttcc catgctgatc ctgccggtgg gcggtctgct
     7501 ggtgggctat ctctccggaa aaaaccggtt tctcattgcc ggtgtatttt tcctgttctc
     7561 tctgatggcg ctcatcacct tcgggacaag tctgctccag gtctccgctg tcatcaacct
//
LOCUS       DAWENO010000094         7557 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_3997_length_7557_cov_7.127699, whole genome shotgun sequence.
ACCESSION   DAWENO010000094 DAWENO010000000
VERSION     DAWENO010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3997_length_7557_cov_7.127699"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2604..2624
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5318..5327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1728 a         1842 c         2060 g         1896 t
ORIGIN      
        1 catcggtttc ggcggtactt ccaacggcat tgaacatgcc gcccagaagt acttcggaaa
       61 gagcgcaaag gatctggatg tggcagaggc cgcctgcctg gcggcgattc cgaaaagccc
      121 ggaaacgctg aatccgtttg ccggctataa ggacgacacc acaggtgaat gggtcaacac
      181 cggcaaacag aaaaacaaaa agcgccagga atatgttctc tggcagatgt acaacaacgg
      241 cgcacttacc tacgatgaat accaggcggc tgtacatgaa aagctggtct ttaccgattc
      301 ggatgagtac aaggctgcac atccggaaac agaaaaacag gaggagaccg acccgaacca
      361 gcccacctcc tggatcgtgg acgccgcatt gtatgaagtg gcagattatc tgaaggaaca
      421 gtttaatctg agtacggaac aggcgatctc ccgtatcaat cgtggcggct atcagatcta
      481 caccactgtg gaccaggata tgcaggcata tgtggaagag aagtataaag atctgggaaa
      541 cctggtggaa aaggaccggg ttgccaagtg ggaagatcag aacggcgacg gcgagtacag
      601 ctcggatgag atcgtttatc cggaatctgc tttcgttgcc atgaactaca agggcgagat
      661 caaggctatg gtaggcgcaa ccggagaaaa gacgcagtcc ctttgcttta actatgccac
      721 catggaacag cgtcagccgg gttctaccat caagccgctg accacctatg gactggcgct
      781 ggaaagcgat ctgatccact ggggttctat ctataaggat gaaccgatcg aagtagaggg
      841 caaggcatgg ccgaccaact acagcgagga cagttcggca atgtctatca gccacaagga
      901 actgaaagtt ttcgaggcgc tggaaaaatc ctacaacacg gtcccggctc agctgtgcca
      961 gacactgaca ccgcagagcg tgtttgactt tgcaacttca aaaatcggac tggatctgtg
     1021 caaggattcc ggtgacggac atacagacaa tgcgtattct ccgctgaccg ttggtgcgct
     1081 gacctatggc gttacactgg aaaatctggt caacggctat gtgccttacg gcaacggcgg
     1141 aacgcagtac aaggcgcatc tggtcagcaa ggttgtccag ggtgccggag atctgatcta
     1201 tgagaacaac ggcgatccgg aagaggctgt cagcagcgaa accgcttatg tcatgaacaa
     1261 gctgctccag gacgttgtca acaacggtac cggtcagaag gcaaagctgg aaaacaagca
     1321 cgttgccgga aaaacaggta ctacacagga ttggaacgac ttgacattcg ttggactgac
     1381 cgaggatttt gtcagcggcg tatggctggg ctataaggaa cgttctgaac tgaaagatca
     1441 ccacatcaag tcagcacaga tctggtacaa catcatcggc gaatatgcag acagcatgga
     1501 cactggtgca gaatacccgg agaacgacaa ggtgatcgaa ggaaaagtct gcgcaagaag
     1561 cggcaagatt gccggaccga actgcgaggc aaccactacc ggttactgga aatccagcaa
     1621 tgcgccggtt tgcgatgaat gcggcaagta cagcaaatcc accaaggcta caggcgaaca
     1681 ggaagagggt acatccgaaa cgactgctgc cggtgagcaa acccaggcaa atcacgaaaa
     1741 tgaaaacggt gacgcagcta ccgaggctgc aacacaacct gcacagacgc aggctgcaca
     1801 ataaaggcaa taccgcacaa gacatcaagc tgccttgtgc ggtattgtat gccatctcac
     1861 ataataaaag gagtatcgat catgcaacaa acttatcttc tctgctgccc ctgtcacttt
     1921 ggtctggagc gcaccctgcg ctttgagatt tccaagatcg gcggcacgga tatagcggca
     1981 caggacggac gtgtgctgtt ccggggcgat acggagacca tcgccaaggc aaatctctgc
     2041 ctctctacag cagaacgtgt tctcattcag ctgggacagt tcaaggcgtt caactttgag
     2101 gaactgtttc aaggcgttcg tgcgctgccg ctggaacagt ggattggaaa ggatgacgct
     2161 tttccggtaa aaggccacgc tctggactcc aagctgcaca gcgtgccgga ctgtcagtcc
     2221 atcatcaaaa aggcagctgt agaacggctg aagtctgttt atcacaccaa ctggttcaca
     2281 gaatccggtg cggcgcatcc actgcagttt accatccgaa aaaacattgc caccatctac
     2341 ctggacacca ccggcatcgg actgcacaag cgtggctatc gaaagaactc caacgctgcc
     2401 cctatcaagg agacgcttgc ggcaggtatt gttgacctgg ctcgtgtcca ctcggacagc
     2461 attgtctgcg acccgttctg cggcagcggt acttttctga tcgaagctgc atttaaggcg
     2521 atgcgcatgg caccaggact caaccgccgt tttctggcgg agcagtggac gcagattccg
     2581 aaagctgtct ggcaggatgc ccgnnnnnnn nnnnnnnnnn nnnnaacatc cggcatgacg
     2641 ctgcatttga ggcgtatgga tttgacgtgg atccggaggc aattgccctc acagaggaaa
     2701 actgccgcaa agccggtgta cagcggcgca tccatctgat gcagcgtgac atttctgctt
     2761 ttgagccgat ggagaatgcc attacgatct gcaatcctcc atacggcgaa cgtatgctgg
     2821 acatccagca cgcccagtct atctaccgca ttatgggcga tgtatttccg gcagacaagg
     2881 cgcatccctg ctacatcatt accccggaca cagactttga actgcacttc ggcaagccgg
     2941 caaataaaaa gcggaaactt tacaacggca tgatccagtg caatctgatg agctattacc
     3001 aggattaagg agcggtttct tatgcagatc gttatgattc acggacagaa tcaccgtggt
     3061 tctacctatc acatcggcaa aatggttgcc gatgcgctgg gcggaacggt gacggaattc
     3121 tttctgccac gggattttgg ctcgttttgc gttggatgca acaactgctt tatgaaagac
     3181 gaaagcctat gtccgcacca tgcaaagctg aaaccgatca cagatgccat ggatgatgca
     3241 gatgtgctga tcctgaccag tcccgtctat gtttatcacg ccaccggtgc aatgaaagcc
     3301 tgtctggatc attacggtta ccgctggatg gtacatcggc ccgaaccggc tatgtttcac
     3361 aagcaggcag tctgcgttgc cactgctgcc ggcgcaggaa tgcgctctac cctgcgggat
     3421 atgcaggaca gcctcttctt ctggggcgtt ccgaaagttt accgcatagg gaaggcagtt
     3481 cacgctgtat cctgggccca tgtttcggaa acacagcggc gcagctgtgc aaaacaggca
     3541 gatgccattg caaaaaagat tctccggaaa aagaaggtac atccgggact gaaaacaaag
     3601 ggatttttcc atatcatgcg catgatacag aaaagcggca gctggaacga aacggatacc
     3661 cggtattgga aagaaaaggg ttggatgcag aaaggtcgtc cctggaagta gtattccccc
     3721 tttacaagca tagaaaagaa aaaacgctga acggttcaga aaaacagttc agcgtttttg
     3781 tggttcgctc aaagctgcgg cggcattgcc ttaaatgttc cagccgactg cgatcagaat
     3841 gatcggcagc agcacaagca atgtattgcg gtacatcttt ttgcgcagtc gtttccattc
     3901 cggcagcgtc attgctttta cacgtggtgt tcccagcaag accagcatcg cattggatgc
     3961 gacataaatg aagtacgcat tgatgataaa cagatagaat gcgcccagca gcatccggag
     4021 attgccgttt gcgatggcat agccgcaggt acagagcggc ggcatcagcg ctgttgcgat
     4081 agctacgccg ggaatgatgt tgttggattt gtcctgtcgt gtctgcccga cgatgccggc
     4141 aatcccaccg gacaaggcga ttatcacatc gaaaaagttc ggattggtac gggccaggag
     4201 ctgttctgtc ggctctttga tcggcgacag cagaaaataa atggtcgcag ccgcaacact
     4261 catgagaatc tgcaccgcaa atccaacaga atgccgtttc atcaaatagg catcgttgga
     4321 cgctgtgccg taagcgattg ccaggatgct gcccatcagc ggcgagatca gcatggcgcc
     4381 gataatcacc gcaacggaat tggtattcag ccccacggag gcaatcagaa ttgcacagat
     4441 catgacgacc atgttggtgc ctgtgacttt tccgccggac aaaatgcggt ctcgtatctc
     4501 ttcattggat gccgcatctt ctgtgatgga aaacatcttc cggaaaaccg ttctgaggtt
     4561 ttcccgttcc ttttccagtt cctgtcgttt cactgtgccg caccttcttt cgatgcaaca
     4621 gtctttttct gaaatgctgt cataagattc ccctttctgt acggttaatg atgttgtgac
     4681 tattatagca caaattgcag ggtgacgtca aatccggcgc agaaaaaaca gagcagatgc
     4741 aaagtgttac cctctgcatc tgctctgttt catttgtgat tttcctttcg gagatgttac
     4801 cggataatat cgctccaggt cacaggaacg cctgcgcccc tgtttgccac gtagtacata
     4861 acatagaaca tatcctgggg atccagccgc ttttctgcgg tgttcttgcc ctcggtgctt
     4921 tcagtatccg catccgcaag gaagtacgcc agaacggaca ggttctcgtc tgctgcatcc
     4981 ggagaggttt ccaggagctt tgtatcggag aaacctgcgc cgacataggc gatatagcgc
     5041 atcagctcgt agatatctgt ggaatccacc ttgccgtcca ggtttgcgtc accctttttg
     5101 ccgatgtaga ccatcggacc ggtgatctcg tatacttccc cattttcggc ggtgtaggtc
     5161 gctttcagat tttctgcgac gtatgccgta tttctcgccg caaacagtgc tgccggcgaa
     5221 tcgtattcca gggtaacgtc tgccgtaacg tccacggttt cccctgccgc attgaccagg
     5281 ctgacgctca taaacgatgc caggtcaaag ggcgtgcnnn nnnnnnngtg cagtagactg
     5341 attctgtcga aatggtaaca gagatctgac cttccggtgt atcggaactg ctggtcgagg
     5401 tggaggttcc cgtttcggtt gtggaagtcg tttcgcttgt ttcggtcgtt ccgcttgtgg
     5461 tggaagtggt cgtggttgtt tctgaagaag ttgttgaagc agttgtggtg gtcgttgttg
     5521 tatttgttgt gctactggtt gcggatgtag ttgttgtgct tgtggttgtt gcacttgctg
     5581 ttgtaatgga agtagtgaca gttgttgtgg tagtggtagt tgctgttgca gaagtggttg
     5641 ctgtagttgt ggtagaagtt gttccgctgg tggttgtgct tgccttggtt gtgaaagttg
     5701 ttccgctggt ggttgtgctt gccttggttg tgaaagttgt tccgcttgta gtagtgctta
     5761 ccttggtcat ggaagtcgtt ccgctggcag ttgtgcttgc ctttgttgtg gaagttgttc
     5821 cgcttgtggt aacgcttgcc ttggtggtag aagttgttcc gtttatagta gtgcttgcct
     5881 ttgttgtgga agttgttccg tttgtagtag tgcttgcctt ggttgtggaa gtcgttccgc
     5941 tggtggttgc gcttgcctta gtcgtagaag tcattccgct tgtggatgtg cttgcctttg
     6001 ttgtggaagt tgttccgctt gtggtaactg tagttgcctt ggtcgtggaa gttgttccgc
     6061 ttgtggtaac gcttgccttt gtcgtggaag tcgttccgct gatcgttacg cttgccttgg
     6121 tcgtggaagt cgttccgttg gtcgttacgc ttgccttggt tgcggaagtt gttccgctgg
     6181 tggctgtgct tgccttggtt gtggaagtcg ttccgttggt ggttgcgctt gccttagtcg
     6241 tagaagtcgc tccgctggtg gcagtgcttg ccttggttgc ggaggtcgtt ctgctggtgg
     6301 cagtgcttgc cttggttgtg gaagttgttc cgctggtgat gacggtggtc gccttggtgg
     6361 tggtcaccgt cgtggtggta gtcgcctgtg cgtttacgat gactgttccg gtcacaacgt
     6421 tatagttgtc gctgtccgcc ggggtaaatt cccaggtcag cgtgttcttt ccggcagcaa
     6481 gccgcttgat ctcgccgacg atcttcaccg tacccttggt gctgttatcg ctgaggatca
     6541 gccccagttc atccaccggg tcgccctcgg tgtgcttgtc gccgtctacg gtcacttcaa
     6601 cagtaggatc tgcctttgca atgtggaagg tcagctccgc ttttttcgat gcgccgtttt
     6661 tcgccgtcag aatcagcgtg gttttcacct tatccgtagc cttttccggt gtgccggaaa
     6721 gcacgccttc cgctgtgagg gtcaagccct ccggcagtgc gcttgccgca tccacctgga
     6781 agtctacgct ttccagaacg tcaaggttcg tcacgaaatc gttcagatcc agcgcatcca
     6841 ttttcttgtg atatgtcagc tcgatggtat cgcttatgcc ctcggaaagc tggatctcca
     6901 ccggcacttt cttcacgatg atctcggtgt cggcggtcag tgtgtacggg ctttctgtga
     6961 tctcagtttc gcccacataa tagtgatagg tgtacgcatt gtcgggcgat ttttccaggg
     7021 tgatcttggc gccggtgttg tgatatgttg tcacggtcgc cgcatcttcg ccaaagcttt
     7081 cgtcataggt catttccaga cggcagacct gctcggaatc ggcgttcagc gtgggatact
     7141 tctcggtgga aagatcctgg tagaatgcaa tggcggactg gtcaccgttc agcaggtagc
     7201 agacttcgcc gctggcgaac tggtcggcag ttttgaatgt tgtgccgtcg aaatcatcgg
     7261 tttcgctatc ggagaggtag tagcagtttt caactgtgcc atctttatca ttaatttgaa
     7321 ctacaccatt catactaccg cctgtaagcg ttccagaatt gtagcaattt ttaattgtgc
     7381 cgccagaatt attccaggcg gctactcctc cgccagtatc cttatcagct gtaatatttc
     7441 cagtgttata acaatttaca attgaaccaa aattattagc aactaatccg ccaacataat
     7501 catactcagc agtgatagtt cctgcgttat agcaattttc aatagttccg ttgctat
//
LOCUS       DAWENO010000095         7543 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4004_length_7543_cov_8.009482, whole genome shotgun sequence.
ACCESSION   DAWENO010000095 DAWENO010000000
VERSION     DAWENO010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_7543_cov_8.009482"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2059..2068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2759..2768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3488..3497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1918 a         1665 c         2149 g         1781 t
ORIGIN      
        1 aaacggctgt gctatgccac cggcgaagat gcgatcatta cctataagca tccctcaaaa
       61 atcgtgcttt cctcggcaaa tgcccggctg atctctgcca acgatgacag cggctttacc
      121 taccgtggac ggttcagcaa taaggaagag gcattcgcca tcggctatga gtattcgcag
      181 aaggtccaca atgcactgaa atggctgatt gctttgcagc ccaaaaatcc tttttgcgac
      241 ctttctctgg tgatctggtc cagcaccatg caggagctgc cgtccattta caagagcgcc
      301 ttgcagctgg cagacaagca ggacgaagat gaggacgagg acgaagaaga tccgcccacc
      361 atcggtacgg catatgccga tatggttcac cagatgatct acggatatcg ggacaaaatg
      421 gagattccgg ataaggtcat ggtgctgggt ttggaggcat cgacggaggg tcgtgtttcg
      481 ataccgatgt atgcagaact gaaaggttca cagttcctgg aaaatctgga atactggcat
      541 ctttccacag ggtggaaaag ctattattcc aaggcgaagg gttatctggt gaactcggtc
      601 tcgctgtatg atgtcattct gtgcgcacac ggggtggagc agaacggaaa gctcacctgt
      661 aaatcgaaac tgttcggcag cgagatgctg cggctgctgc cgtgtatcat ttacggtaag
      721 cacattccgt cagaactggt gcagaatctg gcacagcgtg catcggcacc gcttgccttt
      781 gatcccaaat tctataatca caatcgggtg ctgaaaacag cctgtgcatt gatacggaaa
      841 cagaaactgg aagaacatcc ggaacgattg gaaggagcga atgacgtgga taagttgtta
      901 gacctcgaca aaacgtatac agacagaagc tatctgttcg gacggctgct ggcagttgcg
      961 gacaagctgg aacgggatac atttgatgcg ggagaagatc gggtgaccaa tgccaagcgg
     1021 ctttggaata tgttctccgt tcgtccctac tatacctggg gaatccttcg gggacggctg
     1081 atgccctata tcaacaagct gggaaaaaag gcagaccgct atcagatcga gatgcaggag
     1141 atcagcagca tgatgtctcc cgaagtgttc tccagcacgg cacgcctgtc ttccctgtat
     1201 ctgctgggat acgatcacga gatgaagtat ctgtttacca agaaggaaaa actttcaaac
     1261 aatgaaaccg ctaaaaacgg aaacgaggag gaataactca tgagtgtact ggaacataag
     1321 attgacttcg cagtgctggt cactgccgca aacgcaaacc ccaacggcga tccgctgaac
     1381 ggaaaccgtc ccagagaaaa ttatgacagc tgcggcgaaa tttcggatgt gtgcatcaag
     1441 cggaagatcc ggaacagact gcaggacatg ggcgagaatg tgtatgtgca gtcgaaagac
     1501 catgcccgta ccgatgactt taccaatctg agcgaccgtg ccacgaaaaa tggacatctt
     1561 agccagcttg tcaagggtaa ggataccgac caggatgcct ttgcaaaagc tgcctgcgag
     1621 gagtggatcg atgtgcgcag ctttggacag gtttttgcaa tcaaaggcgg tatctctgtt
     1681 ggcgtgcgtg gaccggtttc gattcaccag gcagtcagcg tttctccggt gaatattgta
     1741 agcctgcaga tcaccaagtg tgtcaacggc gaaagcggca aaaatgccaa gtcctcggat
     1801 accatgggca tgaagcatat ggtggagttc ggactttatg taatcaaggg cagcatcaac
     1861 tgtcagctgg cagagaaaac cggctttacc gaagaggatg cggagaaaat caaagaggcg
     1921 ctgaaaacgc tgttcgagaa tgacagctct gcggcacgtc cggacggcag catggaagtc
     1981 tgccggctgt actggtggga acatgactgc aaaacgccga agtacacttc tgccaaggta
     2041 caccgttcgc tgaaaatcnn nnnnnnnnga tgattatgac atcacactgg aaacactgga
     2101 cggcttgaca ccggaagtga tcgatttggt ataagctgca atggaatatc gggaagagga
     2161 gtatctacag ctttccggca tccagcattt tgtgttttgc cggcggcagt gggcgctggc
     2221 gtatctggaa cagcaatggg tggaaaatga gcgtaccgca gacggcagga tcatgcaccg
     2281 taatgtacat gacagctcgt ttcgggaaaa gcgtggcgat ctgctgatcg tcagaggaat
     2341 gaaagtatca tctgcccgtc tggggctgag cggagagtgc gatgcggtag aattccggcg
     2401 ctctccgaaa ggcgttccca ttttcggaca agagggtact tatacagtaa cacctgtgga
     2461 gtacaaacgt ggcaaaccca aggaagacga atgcgatatt gcacagctca cagcacaggc
     2521 aatgtgcctg gaagagatgt tgtgctgcga cataccggtc ggatttctgt attatgggga
     2581 aacacggcat cgtaccaggg tggagtttac gccggaactg cggaaacgag tggcagatac
     2641 tgccgaagag atgcaccagc tttgccggaa aaggcatacg cccaagggaa agcggacgag
     2701 agcctgcaat gcctgttctt tgaaggatat ttgtattcct gttctgaata aaaagaacnn
     2761 nnnnnnnntg ggaacaatgg agtaaaaatg ccatgaagaa gttgctaaac accatttatg
     2821 tgacaaatcc ggacagttat ctctcactgg atggggataa tctggttgtc atgcgggagg
     2881 aaaagaaagt acggatgccg ctgcacaatg tcgagcgcat catgaccttt ggctataccg
     2941 gtgcaagtcc ggcgctgatg ggcagatgcg caaaggacgg aagagaactg gtctttatga
     3001 caagctccgg aaaatttctg gcacgggtcg agggcgctgt caacggcaat gttttactgc
     3061 ggcggcatca gtatcgggtg gcagatgatg cagatgccag tctttcgatt gcccggaata
     3121 tgatcgtcgg aaagctgttc aacagccgct gggtgctgga acgcatgata cgggaccatg
     3181 caccgaggat cgacacagaa acatttcggc agaagtctca gtttttgcag cggtctattt
     3241 ccaaggcggc agaggcagat tcagcggaca cgctccgtgg catagagggc gaggcggcgt
     3301 tggtatactt ttcggttttc gacgacatga ttctaaagga aaaggcagcg tttgcatttc
     3361 acggcagaaa caagcggcct tcgctggatc gggtcaatgc aatgctgaat tttgcctatt
     3421 cgctgatgac gggaatgtgt tctgcggcgc tggagtccgt ggggttggat ccctatgtgg
     3481 gcgtactnnn nnnnnnnggc gtactgcaca cagaccgtcc gggtcgaccg tctctttctt
     3541 tggatctggt cgaggaattt cgtgcgctct gctgtgaccg ttttgttctg acgctcatta
     3601 accgtggcat gatctatgca gaggacttcg atgtccagga ggacggcgct gtttcgctga
     3661 cggaggacgg acggcgtaca ttcctgcggg catggcaaaa gcgcaaggat gatatgattc
     3721 agcatccgtt tctggaagaa aaagtggaat ggggcttgct gccctatgtg caggcacttt
     3781 tgcttgcacg ctatctccgg ggcgatctgg atgcgtatcc gccgtttttg tggaagtgag
     3841 agcgtatgct ggtagttgta gcgtatgatg taaatacaga gaccaaagag ggaagaaagc
     3901 gcctgcgcca cgtggcgaag atctgtacca acgtgggcat tcgggtgcag aattctgtct
     3961 ttgagtgtca tgttgatgcg gcggaatgca aggtactcaa gcatcggctg gaacaggcga
     4021 ttgatacaga aagggacagc ctgcggtttt attacctggg aaatcgttat cagtcgaagg
     4081 tggaacatat cggtgcaaag gaaaccatag acatggaaga gccgctgatt ctgtgagaga
     4141 agtctgaggg aaaatgtcgg tgcgaatcat aggcgcacag aaagatgctg gaggtttcgc
     4201 accgaatttt tctccctttt ttcgaaaaaa atcatttttt cgggtgcaga atgtagtctt
     4261 ttgcatcctt ttttgcagga ttgtacaagg ataggcgaaa tgaaaaacgc tttcctgtgc
     4321 agttttgctg tctccctcca tacggaggga gtggattgaa atttcaatac gattttccgg
     4381 ctttgtgtat caattgtgtc tccctccata cggagggagt ggattgaaat ccctgtatca
     4441 gccgccacgt caacagcttg gaagtctccc tccatacgga gggagtggat tgaaatcttt
     4501 ccgcaaaaaa tacggatcta tgatacaagt ctccctccat acggagggag tggattgaaa
     4561 tgaaatctca tgtggtgtac acagatggaa aatataatgt ctccctccag acggagggag
     4621 tggattgaaa tgacaaggag gaaaaagaaa atgacgatca aaaacagtct ccctccatac
     4681 ggagggagtg gattgaaatg tgccggaaac gaaagttgtg aaaggaaccg tgaagtctcc
     4741 ctccatacgg agggagtgga ttgaaatacg ccctcaccgg gaaaattaac tgcggatatt
     4801 ggtctccctc catacggagg gagtggattg aaatgtctag tgtacgcaat gccctcccat
     4861 tcccaacagt ctccctccat acggagggag tggattgaaa tttgcttagg tgtgtacaag
     4921 tgggcatctc tatccacggt ctccctccat acggagggag tggattgaaa tgtctacttc
     4981 taacgtcgtt gcgatcactg gcaagtctcc ctccatacgg agggagtgga ttgaaataca
     5041 tatctcgaac aagatgtgca gcagctacaa cagtctccct ccatacggag ggagtggatt
     5101 gaaatgcctt atgcttatgc acaaaacctc ttaccttgtg tctccctcca tacggaggga
     5161 gtggattgaa atgctggtgg tatatccgtt aattcgtggg ttatttgtct ccctccatac
     5221 ggagggagtg gattgaaatt ttgggtccgg tctatactta ccctgctgct caagtctccc
     5281 tccatacgga gggagtggat tgaaatattc ggtgcaacct gctttatcaa ttctcccata
     5341 tgtctccctc catacggagg gagtggattg aaatctcgta cagctgattg ccgatggtcg
     5401 taccggacgt ctccctccat acggagggag tggattgaaa tgacagctac aaatgcactg
     5461 aaccgtggca ggaacgtctc cctccatacg gagggagtgg attgaaatag cttgtcttgt
     5521 tgtgtggtgg cattgctaat atgtctccct ccatacggag ggagtggatt gaaatagcca
     5581 tgctgtgcgg tattccgggc tgttgcgagt ctccctccat acggagggag tggattgaaa
     5641 tatccgacag taggtgacaa tggcacgact gaccagtctc cctccatacg gagggagtgg
     5701 attgaaatga caatttgggc gatctggctg atgcggtagg agtctccctc catacggagg
     5761 gagtggattg aaatggctaa gccttgcccg gaacagcgga ttaaaagagt ctccctccat
     5821 acggagggag tggattgaaa tatcctgctg cccaggatct ctacaagtca accgggtctc
     5881 cctccatacg gagggagtgg attgaaatgt cgctaatcag ttctatgcta acggcaagga
     5941 gcgtctccct ccatacggag ggagtggatt gaaatgcgat atatgcggac atctgatcgc
     6001 ccagctgggt ctccctccat acggagggag tggattgaaa tactgcggca aaaagacgat
     6061 gtgcgtgaca tatgccgtct ccctccatgc ggagggagtg gattgaaatt tttccggctc
     6121 tctcttggtt tctgccgctg tagcgtctcc ctccatgcgg agggagtgga ttgaaattga
     6181 cgatgcaaaa atggcagact ggcagggcga gtctccctcc atgcggaggg agtggattga
     6241 aatgatttcg tggcaattcc aatccgcagg aatttccagt ctccctccat gcggagggag
     6301 tggattgaaa tctttcgcag cggcggcgct agattccagt tctttcagtc tccctccatg
     6361 cggagggagt ggattgaaat gcagaaacct acgaaggcga cgactatgcc ggaatcgtct
     6421 ccctccatgc ggagggagtg gattgaaatg cctcttcccg atccagcttg agggaatgac
     6481 gtacgtctcc ctccatgcgg agggagtgga ttgaaatgat tttcttctat attatgattg
     6541 ttattattac tggtctccct ccatgcggag ggagtggatt gaaattgaac gtgcgtgcca
     6601 tgcgcacaga tacttactgt ctccctccat gcggagggag tggattgaaa ttggaattat
     6661 ggaaaaacgt cccgaaaatc tggcgtctcc ctccatgcgg agggagtgga ttgaaatgta
     6721 aaatccgatc tgtctgattc tgagcgtgaa gtctccctcc atgcggaggg agtggattga
     6781 aatgttgacg gtatcgtgga cggtttcact ggtaaagtct ccctccatgc ggagggagtg
     6841 gattgaaatt ttttccgcag tgcgggcatt tgagattatc atgggtctcc ctccatgcgg
     6901 agggagtgga ttgaaatgca atagagatta tacaagtgcg ttcccatttc cgtctccctc
     6961 catgcggagg gagtggattg aaatgatatg cctgcgcaaa catttcttga tatttctgtc
     7021 tccctccatg cggagggagt ggattgaaat gcttggggcg gcgcacatgg ggcgctgctg
     7081 aaatatgtct ccctccatgc ggagggagtg gattgaaata cgaaagacga tcccggctac
     7141 gatccgtaca tcagtctccc tccatgcgga gggagtggat tgaaataatt gacgtggatg
     7201 atttcgaaca atccgaaatg tctccctcca tgcggaggga gtggattgaa atccggaaat
     7261 gccgtatacc ttgccggaac tggattgtct ccctccatgc ggagggagtg gattgaaatc
     7321 tgccaacagc attcccagaa tcaaggataa gtccgtctcc ctccatgcgg agggagtgga
     7381 ttgaaatcag ttggtgctta ctggtattct tacgccatga gcggtctccc tccatgcgga
     7441 gggagtggat tgaaatgcac tggaagaggg catcgaagaa aacgtgcatg tctccctcca
     7501 tgcggaggga gtggattgaa attggaatta tggaaaaacg tcc
//
LOCUS       DAWENO010000096         7502 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4034_length_7502_cov_9.960387, whole genome shotgun sequence.
ACCESSION   DAWENO010000096 DAWENO010000000
VERSION     DAWENO010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4034_length_7502_cov_9.960387"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    813..822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5492..5501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6177..6186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1538 a         2015 c         2170 g         1749 t
ORIGIN      
        1 aacatcgtcc cgttttgtcg ttatccaaag tatgctgtcg ggtctacata ggcgccgcct
       61 tttttcactt ccaggtgcag atgcggttcg gctgcacttt ccaggtcggg atttgcgccg
      121 gtcacgccca gcttttgtcc ggtttgcacg gtgtcgcctt cccggacttc cagcgtgccg
      181 tccagaccgc agtagcggct ggtgatgccg ttgtcatggt cgatggtgac tgtatagccc
      241 cacagggcat cgctgcacac ttcgctgacc gttcccgtgt cgatggcgaa aacatcagaa
      301 ccggcggcac agctgaggtc aacgccgttg tgtgtctgcc atgtaccggt ggtctcggat
      361 ttcaccagct cgccgtcgct gaaggggttg agaatggaaa aatccgacag cggcggtgtc
      421 agctggtgca tgggtgtttc ttccggttct gtctccggtg cagtcgtttc cggtgtggtc
      481 tccggggcag cggtctcttc tgtagcagac gttgcagtga ctgttgtctc tgtctccatt
      541 gcaacgtctg tctgcacgcc gaccaccggt gcctcatacg gcagggtagc agtggtcgtc
      601 acttccatct gttcgctcag cgagttgaga ttctgttcca gggtctggct ggtctgacgg
      661 tacgcaaaga cacaggctgc gccgatcatg accaggctca gggcaagtgc tgtgtaaaat
      721 ccacggaatt tggatttcgg ttttgtctgg gttttcatgc aaaccacctc cgatgctagt
      781 ttgcgccgga ggaatttttt tatgcagaca acnnnnnnnn nncaaatgcc ggcttgcatt
      841 ttctggaaag atagggtata atagaaaatg ttgcacagtg atgtgcgaat ctgttggaaa
      901 caaggagagg ttctatgaaa caaaaaaagc cgggcagtgt caaacgcctg ctcccgtaca
      961 tgaaagggta ctggaaagag tgtattctcg gtccggtatt caagctgctg gaggcgatct
     1021 ttgagctgat cgtaccgctg gtaacagcga agatgatcga cgtgggtatt gccaaccggg
     1081 atgccggata tatctggaaa atgggcggcg ccatgtttgt gctggcggcg tgcggactgg
     1141 catttgcgct gatctgtcag tattatgcgt ccaagtgtgc ctatggattc ggtacggcgc
     1201 tgcggcgtgc attgtaccgc catgtgaaca cgctgtcgca cagcgccgta gaccgcatcg
     1261 gaacggcatc gctgattaca cgtatcacca gcgacaccaa caccgtgcag agcggtctga
     1321 atatgatgat ccgtcttgca acacgctgcc cgtttttgat tatcggtgcg gcggtcatgg
     1381 caatgcgtat cgacctgaaa ctttctatta tctttctgat tgccattccg gtgatcggcg
     1441 tgctgctgta cagcgtgacc tgttggacga ttccacgtta cggcgagaac cagggcaagc
     1501 tggacaacat cgcacggcat acccgggaaa atctggacgg cgtgcgtgtc atccgtgcct
     1561 tttcccggca ggatgaggag atcgcatcct accggaacga ctgcgatgta ttcgcaaagc
     1621 gctccatcgc tgtgggacgt gtgggcgcaa ttctgaaccc ggcgtcgttc tttatcatga
     1681 atatgggtat cgtggcagtg ctttggttcg gcggcatccg ggtagacacc gggcatctga
     1741 cccagggcga actgaccgca tttgtcaact atatgacgca gatcgcattg tctatggtgc
     1801 gtatggcaga gcttttgatc tccttcaaca aggcggcggc atctgccaag cgtgtgtctg
     1861 acatcctggc agaagagccg gatatcacca acggcaagga gacgcttgca gtcaaggaaa
     1921 atgtgccggt gctgtctttc gatcatgtga cattttccta tccgggcggc ggcgagtctg
     1981 ccatccagga catcagcttt acgctgaatg ccggtgaaac tttgggcatc atcggcggta
     2041 cgggcagcgg taagagtact atcgccaatc tgattccccg gttttatgac accaccgagg
     2101 gaacggttcg ggtatacggc gaggatgtcc gcagctatac cctggaatcg ctccgggatg
     2161 tgatcggcgt tgtgccgcag aaggcctctc tggtgagcgg tacgattgcg gacaatctcc
     2221 gctggggcga tgcggatgcc aatgcggatg aactggaaaa cgcctgccgt attgcacagg
     2281 cgtgggagtt cgtcagccag acatccaacg gactggaaac caaggtgaca cagggcggac
     2341 gcagcttgtc cggcggtcag aaacagcgcc ttactattgc acgtgcgctg gtgggacatc
     2401 ccagactgct gattctggac gacagtatga gtgcgctgga ctacgccacc gacctggaac
     2461 tgcggcggca gctggcatcc aaaatgggag acgtgacgaa gatcgtgatt tcccagcgtg
     2521 ccacatccat tcagcacgcc gaccacatcc tggtaatgga tgacggcaaa tgcgtgggct
     2581 acggcacaca cgatgcgcta ttggaacaat gcccggttta tgcggacatt taccgcaccc
     2641 agatgcagta aggagggaaa gtcatgctga ccgtaattcg gcgaatttta tcgtatacca
     2701 agccgtgccg caaggaactg ttcggaatgc tgttctttgc actgatcgga acggggcttt
     2761 ctctgtttgt ccccattctc atcggtaagg cggtggactg cgtagtcgca gcccacgaag
     2821 taaactttcc gcagctgtgg aagatcattc tggttctggc gctgaccatt gccgtgagcg
     2881 ccgtgttcca gtggctcatg tccctgtgta ccaaccgtct ggcggcacac accatccggg
     2941 acctgcgctg tgatcttttc acccatttgc agcacgtgcc gctgcgctac attgacggac
     3001 aggcgagagg cgatctgatc ggcagagcgg tttctgacat ggagatcatc tccgatggtt
     3061 tcctccaggc gttcacccag tttgtcactg gtgttttcac catcctggga acgctgatct
     3121 ttatgctgac catcaatgtc cgcatcacta ttctggtggt gatccttacg ccgatctctc
     3181 tgctggttgc ggcgaagatc acccagatgt cccgttcctc ttatctgaag tcgtcggatg
     3241 cccgtggcgc actgggcggt ctggtggaag agatgatcgg ttcgcagaag gtggtaaagg
     3301 cgtttaccta tgaagaccgt gcggaagagc gctttgatgc ctataacaca gaattgcagc
     3361 gcaccggcgc aaaggcaacc ttctttgggt ctatcaccaa cccggtgaca cgttttgtta
     3421 atgcgctgat ctacgccgcc gtaggtgtct gcggtgcact ggctgccacc ggcaatgtgc
     3481 cgctgatcgg cgtcatcaca gtcggacagc tggcaagctt tctcacctat gctaaccagt
     3541 ataccaagcc gtttaatgaa atcagcggcg tggttgcaga attgcagaat gctgtggcat
     3601 cggcaaagcg tgtgtttgct gtcattgacg aaccggcaga gtcggacgac agccacctgc
     3661 cggaactgga acactgcgac ggcaccatgc agctgtccca tgtgaagttc tcctatgtgc
     3721 cggacagaaa gcttatcaca gatttcaacc tggatgtaca ctgcggacag cgcatcgcca
     3781 ttgtcggtcc taccggctgc ggcaagacga cgctgattaa tctgctgctc cggttttatg
     3841 atgtggacgg cggcgagatt tccattgccg gacagaatat tgccgcagtg acacgggaca
     3901 gcctgcgtgc ctgctacggc atggttttgc aggagacctg gctgtttacc ggaacggtgg
     3961 cggaaaatat cgcctacagc cgaccggatg ccacacggga agagatcgtt gcagcagcaa
     4021 aggcagccta tgcggacggc tttatccggc gtttgcccaa gggctatgac acggttctga
     4081 ccgaggacgg tagcggcttg tcccagggac agaaacagct gctctgtatc gcccggatca
     4141 tgctgaccga tccgccgttc ctcattctgg acgaggcaac cagcagcatc gacctgcgca
     4201 ccgagcagcg tatccagaag gcatttgaaa agctgatggc aggcaagacc agctttatca
     4261 ttgcgcatcg tctgtccacc atcaaaagcg ctgactggat cctggtgatg aaccagggca
     4321 atgtgctgga gcagggaacc cacgactcct tgatggagca gaacggattc tacgcaaacc
     4381 tgtaccagag ccagttcgca gacagcgaga aggcagaata aaccgaaagg cagcactgca
     4441 cggagtttgt acgctgttga acctttccaa aatagaacac gctcccggtt tccgttatgc
     4501 ctggaaaccg ggagcgtgtt cttatgtagg gtctttcaat gtcggcagat ggtcggggag
     4561 atatcccaga atgacttttt gcagggcatc gtcccagcag aagtagatgc gcaccagttc
     4621 ctggactttt acgccgtact tgatgtgcat attcagcaga tactttttcc cgtcggcggt
     4681 ctgcgccaga tagtcgtggt tgtacacccg cagcgcctct gcgccgcaga acgtgatgtt
     4741 ccagcagtag cgctcgctgt acagggcaaa ggtctcactg tccaggctgc cctggcgaaa
     4801 ccgggcgtag gcatccaggt agaggatgcc gtcgcagata atggcacagt ccggctttcc
     4861 gtcgaatttg cgcaggctgt ttctggcacg ctgggtaaag agaatgtttt tggagaactg
     4921 tttctctgcc catgaaagaa tggcgtcctt ttcagtgggg aaagatgccg ccaggtctgc
     4981 cttttgccga taaaagttga ctcgctctgc cgcctgctga taggcgcttt cccgttcctg
     5041 ctgttcccgg cggagcgtct cgcagtatgt tttcatgccg tccagatcgt cctgcgcagt
     5101 gcgcagctgc ttttgggtgg tgcggagctt ttcactgttc atgcgcatat cggcgttttg
     5161 ctgggtgaat tcccgggcct gttttttcag ttcctcgttt tcctgctgta agatgcggca
     5221 gcgttcttcc agggagaggg tctctttctg gttttcctgg agtgctgtga ttttggcatc
     5281 ggagtagaac aggatgtccc cgaacgaaaa ggcgctgcgc aaaagcatct ggcgcagttc
     5341 ctgcttgcgc tttcgtgcaa agtcctccat ctgcttttca tactgcccgt aggaaaaccg
     5401 ctctgccggg ctgccgtggc ggagaatgag gaagtctccg tttcggagcg acagcttgaa
     5461 cttccgggaa aactcttcca gacagcgctg cnnnnnnnnn ncgctgcgcc acaaaacaaa
     5521 ctgcgccgta tcccagcagt ttttccgcca gatcttcata ggggaatacc ggcagtttct
     5581 ctgtgagacc agaacctgca ctttccgttt tctcggcatc tgcccggttc atgccggtca
     5641 tccgtttgag tcgccgattg ggcggaatgt gcatctcctt ggtgatctcg gaagttgcct
     5701 cgttcatctg gacgttccgt ccggaaaaat gcagcaggtc gttctgcgtg gagcgcagcg
     5761 aaagcgtgga cttgtcggga atgttcagcg catccaggga aagcgttttc tgtgctgtgc
     5821 cgctgtctgc aatgacgatc agcggcatat caaaatcggc agcctggtac agtgcggcaa
     5881 agtgggcgaa gtcaccctta gcgttgattt ccatgtgctt tccgtccagc acaaaaccgt
     5941 tctggacaaa ccctacattg gggttctgtg ccagcgcccg aaccaccatg ggacggaaca
     6001 cctcacaggg tgcgctgctg tcagacggct cagagcagat ggtgcggata cctacttcga
     6061 cacgttcctt gtgcatcaga aaagagatgt ccgtctcaaa gattctgccg ttgactgcgg
     6121 cacgttctct gggtgtgccg aggttggcac cggcatccgg ttcagacaga tggaacnnnn
     6181 nnnnnngtgg aaaaatcggc gtagttttcc ggcttcgggg tgcagatgtc tgccggcagg
     6241 ctgtcgaact tttgcaggcg gctgcgcagc caccgcagcg tttccagcac acagatgcag
     6301 aacacctgtg aaacgggaag tttcttctgt gcggtgtcgg cgtgaaactg ataggtggga
     6361 tagcgtttga tcggctgtaa attggtagtc tggtatttca taagcatctc ctcacagcgg
     6421 ctgatagggg tcaatgccga cggcatcaaa tccggcgatc tcgtcatagg cggcaagggc
     6481 aaagtcgaac agctttgcag cgatcacgcc gcctgttcct tcgcccaggc acatttcaca
     6541 gtccagatag gcacgcagat gcagtgcgtc cagcgccatt tttccggctg gctccttgga
     6601 acagtgggag gcgaagatat agtcccggca cgccgggcag agccggacgg cgcagtttgc
     6661 cgcaactgcg gaaatgaatc catccaccag caccggcacc cggcagatcg ctccgcccag
     6721 aaatgcgccc atcatgccgc agacgtcgaa cccgccgacc ttggaaagca cgtccagcac
     6781 atcgccgggg tctggctgga gcgctgcgac ggcacgggag atcacctgga tcttatgctg
     6841 caatccagcc tcggataagc cagccccacg tcctgtgacc tgctccggcg gcaagccctg
     6901 cagcacagag aaaacggcgc tggaagtagt ggtgtttccg attcccattt cgccggtaac
     6961 gataaggtta tatccctccg ccgctttttc ctgtaccagg tcgatgccgg tacagaccgc
     7021 ctgttctgcc tcatcccggc gcatggcggg accgtgggca aagttctgcg tgccgaaggc
     7081 gaccttgcgg ttcagcacac cggggcagtc cagcgcctgt gcaacgccga tatccaccgg
     7141 gaatacctct gcgccgcaga tacgggacag cgcattgacg gatgcaattc ctttggcaaa
     7201 gttttgcgta acggcggcag tcacgtcctg cgtggtctgg gtcacaccct cggcaacaat
     7261 gccgttgtcg gcgcagaaga tcagcaccgc ttttttgcgt ggggcaggca cgggattttc
     7321 gcagatgccg gcaagctgta cgatcatatc ttccagccgt cccagactat gcagcggctt
     7381 tgcaatggcg tcccagtgca gacgggcgct ttccatggca ttttcatcca gcggccgtat
     7441 ggtttctagc aattttgtca gcatagatga cagttccttt caacgtgata tatgagatgt
     7501 at
//
LOCUS       DAWENO010000097         7455 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4065_length_7455_cov_11.151486, whole genome shotgun sequence.
ACCESSION   DAWENO010000097 DAWENO010000000
VERSION     DAWENO010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4065_length_7455_cov_11.151486"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2143 a         1771 c         2038 g         1503 t
ORIGIN      
        1 gccttatgaa aacgacacca aagcaaccga tgacaccgag cgtaaatcca agtggaacgg
       61 ctatctgatt ccggagtctg tccagctgaa aaggacctcg tccggcggta cgtcatcctc
      121 taaggttgac ccgtcagaat atacagtctg ggtcggaccg attaccaaga agaacggcag
      181 ctatgaacta caggatgttg atgcactccc gaaaccagca gacggtttgg atagtacgca
      241 gagaaatgaa ttttatcgta acctgtatca aaacataaac aaaaaggagt cgtattttgt
      301 caagctgtcg gatctgatga gcgaccatga aaacggcaag atctctgatg cagagtatga
      361 caagctggtg cgtggcattc gtgcaatctg ggcgcagatg aataatgcgg ataccatgct
      421 gagcaagcag agtcgtatgg aactctacta tgagatgcac gcaccgctga atctgcccaa
      481 atatgtgggt acgccgacag tagatgatga aaacgaaaat gcgttgtcca ccgaaacgat
      541 caaaaagaaa gtggagggtg taactcagtg gaacaccttt gcttccggtt acagctctcc
      601 gacatctgcc gacaagagtt accatgtatt tgaaaacaaa caggctggcg cttatatttc
      661 cgcacccagc ggaaagggct atatcggcag ctatgtctgg agcgacttca attatgatgg
      721 tcagtgcaac gaggcggaat atgagatgtc tgccaacgga cggctgctgc ctaccggaaa
      781 gtggacaacg gatattgact tcgacggaaa gccggacgat ccgggtatca acggcgtaaa
      841 ggtagagctg ctgaccaaga agggctactc tgtcaacgtc aacggtgagg cagtgattgc
      901 cgatctggac cccgacagca acccgaatcc ggaaaaggtt cgttatattg tcattgacga
      961 ggagaccggt cagccaaaga ccattgtaga aaacaacacc aattcctatc agtatacgtt
     1021 tgaaggtccg gttgtttata ccaccgaaag cgactattac ggcaaccagg gctacttcat
     1081 cctgtcgaat ctgacaccgg gtgattaccg gctgcggtac acgctgccga agggatacga
     1141 ccagtattcg gttaccacac gggaacttgg cgagtcaaag acaaggctca aggtttaccg
     1201 tgacggaaag gtcatctata atggtatgag tcatgctgaa gatgatgcag taatttccaa
     1261 taccaacagc gttgaaaata tcgaatcgtc tgtgaatcag cttgtcgtac agactgcact
     1321 gattcacgtt gatgcggtgg aagaagatcc ggcaaagcac caggcttatg acactgccat
     1381 gacatcctat atgctgggtg tctccagagg ttatacctac ggcggctggg catgggtcga
     1441 tgaaacagac aacaacggca ccatcgaaag cgacggcatc atgcaggaaa ccgaaaagaa
     1501 actggaagat gtgacggtgg aaatgcacga ggtcgatgca gacggcaata tcgcatcgaa
     1561 agctgcggta gacggtgatg gcaatccggc aattttccac actggggatg acggctatta
     1621 tcagttccgt ctctatccga acaagaacta tgtggcaatt gcaaagtatg agggcagtga
     1681 atctaccccg tataagcctt ctccgtttgt gctgcacaac gatccgctgg aggctgcgga
     1741 tgacaacgat ctgaccaagg cagtgaacaa cttccgcaca cggccgttcc agaccggtgt
     1801 gccttatgac gcaaaccagc agccgctgtt tgaaaatgac ggacagacct tccagatcaa
     1861 caagagcatt tctctgggat tcgtggacgg ctcccgtgga tttatcggac agtggatctg
     1921 ggacgatgaa aactacaacg gcatccggga tacttttgaa aagggtatcg agggtgtgac
     1981 cgtgaccttg aaaccgttct attacgacag cgctactgga aaatgggtag cgattaccgg
     2041 tgaagaccgg gatatcaaaa cctcggaatc cggttcctat gtattccaga atgtttcgac
     2101 ttattacaag aaggacggaa agacgtacct ggctggctat cggctgtatg tcgatccgga
     2161 gaagaacgct gatctgtaca aaaagtatgc gatcaccaag tataagcagt atccgagcag
     2221 ccgctctgcg gaaaacagtg acctgcaata taccggcgat atgcagtact atctcattgg
     2281 cgatgactgg ttcgagtatg acgcaaacgg cactgtcaca gcaacgcacc acaactatga
     2341 cacagaagat aatatcatta tcattgccgg cgagacgaca aaccagaaca ccaatgtcat
     2401 tgactataag gaaacccatt acgataccgg cgaagcacag cgtctgctgg attacagcgg
     2461 cggctttacg gaaattcaga cttctgaagt aaccggttat ctgtgggagg acaacggtaa
     2521 aaatgcagcc ggggaataca aggaggcatt tgactatgac ggtatccgca acacggtgac
     2581 cgcagaggat ggcacgaccc aattctacga aaagggaatt gccggagcga ctgttcagct
     2641 ggaacagtat gtgctgcaaa acggcagcta tcagctggtt cacacctacc aggtaaaaac
     2701 cgatgcagac ggcaagtaca cattctctga tgtttccaca tatgcggaaa tcaatgggga
     2761 aaaggtgctg gcacactatc gtctgaaact gctgtcgctg cccagcggta tggaaaacta
     2821 tgccgttacc cgctatcggc agaacggcga tgcggaaacc caggtggtgg acagccactt
     2881 gatctcgcag aacgagcagc acggcgcagg ctacaccgat tatgacattg cgtcttatct
     2941 gacctctccg gcggacggtg actatttcat caccgcaaag aagtctcagc cggagacagc
     3001 gccgggcatt gacggaaaaa cctattttga accgtacatt ctctatgatg atgcggcagc
     3061 gaacaacgga aatcatgatt atgcctacga ttacatcaag gcaaaggatt atgacggcta
     3121 tgacggcggt ctgaaagcat ttgaaactgc ctctgtcagc ggcgtggtct gggaggatac
     3181 caactataat ggtgtccagg acgacgatga agcagcatac tatgacagcg aaaatccggc
     3241 agtcgtacac ttgcagcagt atttctatcg caatgacgaa tgggtgccgg aaagtgcagc
     3301 atttcggata cacgaaacca agacagacgg caagtacacg ttcagtaatc tccccaccta
     3361 tgtggaaaag gacggcgtaa ggtatctggc aggttatcag gtgattttga aaaaattgcc
     3421 ggatgattat gcggcaacga agtactggag caatgcagac ccgtcaaagg tttacagcaa
     3481 gctgtatgtg accgatgccg acgatggcac cgaggacagt cagcggacag gtgacctgcc
     3541 gatcactgtg ctgaagggca atcacgaaat gctaattctg gcacaggagg caaaggcagg
     3601 cacacagtcc aacagtacag aaccgtatat cgattacaac tgggcatatg tctgcggcac
     3661 gaacatcaaa aacatcgaca ccccgaacac cgcacagcag aagtactacg atctggtgaa
     3721 ggcaagagat tatgctgatc tgaatgccgg tctgaaagag cgtggcgttg caacggtaaa
     3781 gggtacgatc tgggaagata cggactacaa cggtatcctg gacaatggcg aaaagggcaa
     3841 gagcggcgtt tctgtcactc tggaacagta tcgctatgat gtcgatgcaa agcagtttgt
     3901 caagatgggt gcagacgaaa cagtcacaac tgacaaggat ggcgtatatc aatttaccaa
     3961 ggtgccgacg cactttgtga tcggcggcgt agaccagctg gcatattacc gcattcgtgc
     4021 ggatgtgccg gagggctatg cggtgacacg gtatcgccag gcaaccagtg acgcagaaac
     4081 agacagcgac tggatcgcag tgaccaacta tctgactgca ccgggcaacg gggactactt
     4141 cctgacggca cagccgtcac agggcaatgc tccctatgat ctgactgatg ctgtcacaaa
     4201 caaggaatac gattccattt tgaatgcgga cgtgatcgac gtttacaacg gtggtctgaa
     4261 agcgttcgag accggaaaga ttcaaggcac tgtctggttc gacaaggact acagcggctt
     4321 gcaggaaacc ggtgaatcgg caatgccgac acagcagacc gtatatctcg accagtatta
     4381 tctggacgat gccagacagt gggagtttgt caagacaggt tccgttcaga cagatgcagc
     4441 cacaggtaaa tatcagttct ctgaccagcc gacctttgtg acggttgacg gaaagatgta
     4501 tctggcaggc taccgtctgc ggttgaatgc cgtgccggat ggctatgcgg tgaccaagta
     4561 ccaggtgctg gattccacag gaaactgcat caacagtgac ctgctggcag catctcagga
     4621 aacgaatgca ggtaagacgg tagacttcaa caagccggac gagtatatcc tgctggcaaa
     4681 gcaggcggcg catggcgttg gcagcattga atcgggactc cacagttact atgtacggaa
     4741 gtacaacaat gtcgattatg atattgtcac tgcggtggat tccgacggcg tatacaacgg
     4801 cggactgaaa gcgattcaga ctgcggcaat ggacggacga atctggaatg acctcaatta
     4861 tgacggcacg atggatttga ccgaaagcgg ctgcagcaat ctcaacttga cactgtacca
     4921 atattattgg gacggaacga catggaaaaa gacacaattt aagaaacagg cgacgacaaa
     4981 cagcaacggt cagtatcact ttgacggcat cccaacttat gcggaagtcg gcggcaagcg
     5041 gtatttggct gggtatcagc tgcatctgga tgcactgccg gacggcaatg cggttaccgt
     5101 attcgccaac gacaacaagc tgcactggga caacggcgaa cttactttga tgggcgagca
     5161 ggagtatctg atcgttgcag cagaggcaaa gtcgtttacc ggagagtata ccggaagtac
     5221 gccgcagtac aacgagtact atgaccgcac ttaccgtgca gttccaggta cagatacgat
     5281 ctatgacatc acggaaaacc gtgacagcaa gaacgagtac catggcggta tccgggcatt
     5341 ccagaacgca tccgtcagcg gacgaatctg gaacgatgcg gattatgacg gcgatatgac
     5401 atctggtgaa accggcatgg aaggcttgtc gatgacgctg gaacagtatt actgggacgg
     5461 cacatcatgg acaccgaccg cacggccgaa ttacaccgca accgtaaaaa ccgacagcaa
     5521 cggggaatac cagtttgatg atgttcccac atttgtggag gtcgacggca aacgctatct
     5581 ggctggctat cggctgaaac tggacgcact gccgaaggac gctgacggca agatcaccta
     5641 tggcattacc aaggcaggtg gtgacagcaa gttccaaaga cttgaaaacc cgtatcagag
     5701 cgagaacctc tatctgacag caccggatga atacttgatt ctggcagcag ctgcacagcc
     5761 aaaaacagat ccagaatacc gaaagaccta caacggcagc gactatgaca ttgcggatgc
     5821 tgcagaccag gcaaactgga acggcggttt gaaacaggtc gaaaaggcgc agatcgttgg
     5881 acgtgtctgg gacgatgcga attacaacgg cttgcaggat accggcgaag gaaacatttc
     5941 cggcgtagaa attacgttgg agcagtacta ctgcgatgcc aacggcaact atcagcctac
     6001 cggcaaatcc ccccagaagg atcatgatgc caacggcgag taccggtttg acaatgtgga
     6061 tacgttctgc aatgtggacg gcaagaccta tctctgctat tatcagctga aaatgacagg
     6121 caaagtgccg gacaattaca ccattacctg gtaccggcag ggcgatgatc ccgcaaggga
     6181 cagcgatctg gatgcggaga gccgttatct gacgaaagac acctattttg tttgtgcagc
     6241 tgcggatacg gacagcacgg attatacagt ggacggcaga gatattctcc gaaaggaaga
     6301 tgtttccggt tatgatgccg gctttacaca gttggcagta ggggacatct ccggacggat
     6361 ctggatcgac catgattaca acggcatcca ggataagtcg gatgatgatg ccaataccgc
     6421 agaggacaag gcggcaattg caaatgtcac cgtcagggca atccagtatt actacgacgg
     6481 cagccagtgg cagccgaacg acacggcgta tacagccagc acaagcacag atgcagacgg
     6541 caaatacaca ctggcgaatc tgcccacctg ggtgagaatc aacaatatgg attttgccgc
     6601 aggctatcag ctttgcctgg aagatctcca cggcaactat ctggcgacac agtatcgtgt
     6661 gggaactgac cgcaccaagg acagcgacct cagactggac agcgcatatc tgacaagcac
     6721 aaatgaattt attgtaccgg cggcagatgc gtacagcgat gccgacggaa accagaaccg
     6781 tgcatccgta cgggaagtgc cggatgcaaa cggtacgatg taccaggtag acctggtccg
     6841 cagcaagaat accggacatt atgatgccgg actcaagcag caggacaagg gtgttatccg
     6901 tggcacgatc tgggacgaca agaactatga cggtttgtac aatctggacg aagtgccggt
     6961 ggcgacttcg gttcgtgtga tactgaaacg tgacatctat gagaatggaa aatgggtacc
     7021 ggatgataca ttcggcgaag tttccaagat cgtggacaac ggttcgtatc agtttgacgg
     7081 tctggatgtt catgtgccgg gagatctgtc tgacggcagc gctgcggaca acaaggaccg
     7141 catctatggt tactatatcg aactggatct ggcaactgtg ccggatacct atgcggtgac
     7201 caagcagtac gcaaaaccgg atcgggatca gctgaacagt ttcctggatc ctgaggacgg
     7261 cagtctgatg gcagaggacg aatcacatat tattctggca gctaaggcaa cagaaggcgt
     7321 gacggaacat actgtcacca tcggcgatgt aacatatgcg ccggcggatc tgtcgaaatc
     7381 catcgcatcg cataacggcg gtttgacgaa gatcgaaaac gcatccattg acggctacat
     7441 ctggaacgat gccaa
//
LOCUS       DAWENO010000098         7429 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4074_length_7429_cov_9.127882, whole genome shotgun sequence.
ACCESSION   DAWENO010000098 DAWENO010000000
VERSION     DAWENO010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4074_length_7429_cov_9.127882"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1898 a         1917 c         1830 g         1784 t
ORIGIN      
        1 gtacatttca ttatagcaca aaaccgatgt cgttttcaat agctattcaa aattttatct
       61 tgatttttga aaaaaaatta tgtataatgg tatgtggtat gattggaaca gtaccgtttg
      121 ctctgtcttt gactggagaa agcggttttg aatacaaatt cccttcacag aaaggaacgc
      181 aacatggaat taagtgaaat tcgcaacgaa atcgacaagc tggacaaaca gattcagaac
      241 ctatttgaag agcgtatgct cctgtgccag gatgtggcac gctataaaaa ggcacacaat
      301 atgccggtat ttcaagccgg acgggaacag cagatcctgg atcgggtaga aagcaatgcc
      361 agagacggac tgggtctggc atcccgtacc atgttcgcca acatgatgag cattagctcc
      421 cagctacagc agaaaatgct ggtcgctgcg gaagatgcac cggaattcaa aatgccccgc
      481 atgaaaaatg ctgtccgcat cggatgccag ggaacagtcg gcgcaaactc tgaaactgcc
      541 gcacaggcgt ttttcccgga gcggaacatc cagttctacc ccacttttga acaggtgttt
      601 gaggcggtgg agtccggcga actggaatac ggtgtgctgc ccatctacaa cagcacatcc
      661 ggcacggtga cacagacggt tgacctcatg ggaaagtaca gtttcttcat caccgccatg
      721 aaccaggtag aagtggcaca ctgtctcgcc gcactgcccg gcgcatcctt agagagcatt
      781 caaaacgtct actcgcatcc gcaggcgctg tctcagtgca gtgacttcct ggatgcacgt
      841 cacctgcatc caaaggattt cagcaacact gccactgccg caaaaatggt ggcggaatcg
      901 ggcgatacca cgatcgctgc gatctgctct gaagaatgcg ccaagcacaa tcatctggaa
      961 attctggcat cccgcatttc caatgttgta ccgaatttca cacagtttat ctgcatcacc
     1021 aaggatatgg aagtggcaga ggacgcatct gtcatctccg tcatgctctc catcccgaac
     1081 gaacccggca gtctgagccg tattctgaac aagttcttct tatacggtct ggatatggtc
     1141 aagatcgaaa gccgaccgat ccgggacggc agtttcgatg ttatcttcca catcgacttc
     1201 aagggaaatc tgagagaccg cagcattgcc gcttttctga acgacctgtg caaaagctgc
     1261 cgggatttca agcttttggg aaatgcggtc gtgatggcat aagacagcac atcattgcca
     1321 actgcaagca gcctgcaaaa aaatgcaggc tgcttttttg atgccgatat cacattttcg
     1381 gtgtatcgcc acgcatcagc gtagaacgat attcggactc ccggaactgt ttcggtgtca
     1441 caccgacttt tttggaaaac acctgaatga aatggctctg cgaagaaaat gccagcagat
     1501 tggcgatctc cgcatagctg cgctccgaaa actggagcat ctgatccagg atgtagtcat
     1561 gcagcgtaca gcccatctgt tcccggaaga gccgggacaa atagctggga tgcagatgca
     1621 cttcctccgc caggtcttcg gtggtgatct tctcgtgcat atgcgcatag atgtaattta
     1681 ggcaaagcgt aaccggtttg gaacagaccg gcttattttt gatctgctgc atccgttttg
     1741 catagtccat gcacgccgca tcgtgcagtt cctccaccag atcaatggta gttacctcgt
     1801 ccaccttgtg gatatagaag tcgctcatcc ggtatgcctg ttctcccggc attccgccct
     1861 cgatgcagaa acgagttacc attgccagag aaataataca gtgataccga aaattctgca
     1921 ccaaatctgt ggaaagtctg gttttcatgg caatgtgccg gtattgtccg gactttagat
     1981 ttttctcaac gctcgccaca tcgccctgct gcacctggtt atagaacacg aattccacct
     2041 ccggcggttc gtgctgtata aagtcctcct gccgctcctg ctctgcattt tgccattctg
     2101 tttcgatttt tgtcatttca gtaattcccc catttgaaaa tatgaaaaat acgcaaattt
     2161 tattttccca cgaaatctgt cccagaatct taacggtcat atttcttttg cattccgcaa
     2221 ttttttgaca aaacgaaaag tgatatacca aaaagttttc tgccgcatcc cacagattcc
     2281 ataatgctat accagaaaaa agcaagcaac atccaggaat tttccattgg tttgccattc
     2341 cggaagatgt tctttcctct gttgtaatat tatatcatat ttctataaaa aaagcaagtt
     2401 ttttggtatc atttttttga tattcctcgt aaaatgaaaa tgcagataaa tcttgggagc
     2461 agtgtcctat ggaataattt gggcgattcc agctgttccc gaaagctcct ggttctattt
     2521 gaaacaaaag gctactgctg gaacatactg cataccgtat gaaaatatgt agtacaggca
     2581 atgagacaca atatgttcca atctatgtga caggaggtca tcatcatgcg aatttgggaa
     2641 ggttttcagc atggtatcaa ttttggcgga tggctctcgc agggagaaca ttccgacaaa
     2701 cactacgaaa catttttgac agaaaccgac tttgcagtcg ctgcacgctg gggactggat
     2761 catgtccgta tcccggtaga ctatgaactg gtcgaaacgg aaggcggcat tcaaaaagaa
     2821 agcggatttc agtggatcgc acagtgcatt acatggtgca gaaaatatgg tctgcacatg
     2881 atcttggatc tgcacaagac agccggatac acctttgacg atccgatcca ctccgatgaa
     2941 tttttcacca acccggcttt gcaggctcgt ttcatttctc tgtggcagga attcgcaaag
     3001 cgctacggca aggatcacga tgttgtggca tttgaactgc tgaatgaaat tgtcaatgca
     3061 gaatcggcag agtcatggaa ccgcatcgcc gcaaatgcag ttgctgccat tcgttctgtg
     3121 gcacagaaaa actacattct gatcggcggc attcatcaca acggcgccga cggcgttccc
     3181 cttctggatg caccggcaga ctccaggatc gtgtacaatt tccactgcta tgagccgatc
     3241 gtctttaccc accagacagc atcctggaca aagggaatgc cacggaattt ccacatcaag
     3301 tatccggatg atctggcagc ataccgccgt cattccgctg cattcaacaa gcagtatatc
     3361 gaggcttttc tccacgaagg cattcaggat gtagaggaat ccttcttcga ggcaaacttt
     3421 gccccggcaa tcgcagcggc tgaaaagtac gatattccgc tgtactgcgg cgaatatggt
     3481 gtaatcgacc aggcggatac ggaaagtaca ctgcactggt accgcaccat cacatccgta
     3541 tttgacaagt atcacatcgg tcgtgccgca tggacatata agggaatgga cttcggtctg
     3601 acggatcctc atatgaatcc cattctgccc caggtactgc ccctattgta agtaaatcat
     3661 atcaagtcaa gctgttctat ccgctgaaat gccggcaggg cagctttttt gtttccccgg
     3721 aaatgcacac tttacaagcg gcaaaattcg tggtataata taggcagtac tattttataa
     3781 atagtgttgc tgaaaatgta cacaacagac gatggaggtc catatggtaa caaacgacgg
     3841 cacgatcatc aaaatcaaac acgatatcct atgtgaggtg gcaaagctgg tctttgccgg
     3901 aaaatttgaa gaggaaaaag aagaactccc ctaccggatg atgcccggtc cgaaagcaaa
     3961 ataccgctgc tgcgtctata aagagcgtga gatcgtccgt cagcgcatcc gcctggcaga
     4021 gggaaaatca ccgcagggtg cgcacaacaa ccttgtggta caggtcgtgc aggctgcctg
     4081 tgaggactgt cccatctccc gttatgtcgt taccgacaac tgccagaagt gcatgggcaa
     4141 ggcctgtcag cagtcctgca acttcggggc aattgatatt ggccgcacca gagcgcacat
     4201 tgatccgcag aagtgccggg aatgcggcaa atgtgcggca gcctgtccct acaatgccat
     4261 tgccgatctg attcgcccat gtcgccggag ctgtcctgtt ggtgcgatct ccatggatga
     4321 aaacggcatc tgtatcatag atgaaaagaa gtgcatccag tgcggcagat gtattcacag
     4381 ctgcccgttc ggtgcaatca gttccaagtc ctgtgtggtt gatgtctgtc aggcgatcgt
     4441 tgagaaaaag cacgtctatg ccatgtttgc accctccaca gagggacagt tcggtgaggg
     4501 cattacattt gagagctggc gcaatgcgct gaaagaagtt ggctttacag acctggtaga
     4561 ggttggactg ggcggcgata tgactgccta tgccgaggcg gaagaatggg ctgaggcata
     4621 tcgggacggc aaaaagatga ccacgtcctg ctgccccgga tttgtcaatc tgattcgtct
     4681 gcactacccg gatctcctgg atcatatctc tacaacagtt tctccgatgt gtgctgtttc
     4741 ccgctggatc aagtcaaaag atccggaggc gatcaccgtt tttgttggtc cgtgcattgc
     4801 aaagaagagc gaagttattg accagaacgt ccccggcagc gctgattatg caatgacata
     4861 cggcgagatc cgtgccatca ttcgtgcaaa gggcataacg atccatgagg cagaagagac
     4921 caaccagcag tccagcacct ttggcaaatg gtttggcaac agcggcggcg taacagatgc
     4981 tgtcctggag ggcatgaagg aacaggggat caccgacgaa gtaaatgtag aacgctgcaa
     5041 cggtacagat gcctgcaagc gtgcgctgct gatgctgaaa atgggacgct tttccggcga
     5101 ctttatcgag ggcatggcgt gcgacggcgg ctgcgtcggt ggtccgagcc gtcacggcga
     5161 agtgaacaag ttcaagcgtg ccagagaaaa catgataaag aaggcggaca agcggaacat
     5221 tcacgaaaac ctggaaaagc tgggggcaga aaagattccc atgcaccgca gcggctggac
     5281 agaaaataaa taaaacagca acttaacaga aagcaaaaca gctccatgca agcggcattg
     5341 taccgccgga catggagctg tttttgtatt tttcagcgtt tatgtcacga attcgcctga
     5401 ttcttccgat atgccagata gctttccaga atgatggtag ctgcaacggc atctactacc
     5461 gccttccgct tttttcccct ggtgtttgtg acattcaggt aggaatgcgc cgaaacagtt
     5521 gtacagcgtt cgtcccacat gacaaccgga ataccggtca tttctttcag accgtctccg
     5581 aatttctggc acagttctgc acgttcgccg actgtaccgt tcatattctt cggatagccg
     5641 accacgagaa gttctgcacg gtgttccttt gccagagcgg caaccttttc ctggcactgt
     5701 gtgaaatcct tttccgaaac cacacacacc ggagatgcca gcatttcgcc cttatcacaa
     5761 atggcaagcc ctgtccgggc atcgccaaag tctacgccca taataatcat aagatgtttt
     5821 cctcacttac cacggctgca ctgtaccgat gatttcctgt accgatgcaa tattgtgaga
     5881 atccagccag tcgttcatct cgtccacaat gcgtaccggt gcatagggat ctgtgaagat
     5941 cgctgcgccg acctgcactg cgcttgcgcc tgccatcatc atttcaatag catctgcacc
     6001 gctggatacg ccgcccatac cgattaccgg aatggaaacc gcctgggcgg tctgccatac
     6061 catacgcact gcaatgggga aaactgccgg gccggacatt ccgcccacgt tattgcgcag
     6121 gatgggacgg cggttttcga tattgatgcg cattccgagc agcgtgttaa tcagcgaaac
     6181 tgcatctgct ccggcagatt ccactgcctt tgcaatatcg gtaatgctgg taacattcgg
     6241 agaaagcttg acgatcagcg gctttttggt cgctttccgc actgccgcag taatcatttc
     6301 cgcactgtca cagtgtacgc cgaatgctgc gccgcctttc ttgacgttcg gacaggagat
     6361 attcagctcg atcatatgca cgtcagtctc atccagcttt tccgcaatgg aaacacactc
     6421 gtcaatggta gagcctgcaa tatttgccag gatcaccgtg tcctttgtga gcaggttcga
     6481 cagttcccgt tccagaaacg catcaatgcc cggattttgc agacctaccg aattcagcat
     6541 tccgctgggt gtttcagcaa tacgaggcgc cggattgccc ggacggggct gaccagtagt
     6601 gcctttggtt gcaatgccgc ccagtttgga gagcgggaac agcttttcgt attccctgcc
     6661 gtagccgaac acgccggatg cagggatgat gggatttttg aacgcaacgc ctgcaacttc
     6721 aactgctaaa tttgccatta ccaaacaacc tcctctgccg ggaataccgg gccgtccttg
     6781 cagacccatc acggatttac cttccttttc cagctggcag gcacagacaa gacacgcacc
     6841 gatgccgcag cccatacgct cttccatgga aacctcacag cgcacgccgt attccttggc
     6901 agcagctgct acgaatttca gcatggggcg aggaccacag gcatagacca tatctacacc
     6961 gccgccggaa agctgctctt tcagcggttc tgtcaccagg gcgtgtctgc cggcagtgcc
     7021 gtcatcagta cagagaatgg tctctgcacc agatgcgtgg aatgcgtcct gcaatgtgac
     7081 aatgtttgcc gaacggaaac cgcagatcgc tgtggcacgt gcgccgtata tctgcgccag
     7141 ggacagcata ggcggattac cgatgccgcc gccgacaacg acaacgtgtt ttgcatccgg
     7201 caaaatggtg aatccatggc cgagcggtcc cagaatgtcc agattttcgc ccacacgcac
     7261 attggcaagg gcatctgtgc cgctgccctt cacttcaaac acaatggtca gcgttccggc
     7321 gctcttgttg atctcacaga tagagatggg acggcgcagg gtacagcctg tgggaagcag
     7381 atgcacaaac tgtcccggct ctgccttctg tgcgatagaa cggcactgc
//
LOCUS       DAWENO010000099         7418 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4083_length_7418_cov_9.194350, whole genome shotgun sequence.
ACCESSION   DAWENO010000099 DAWENO010000000
VERSION     DAWENO010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_7418_cov_9.194350"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4385..4394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1668 a         1817 c         2302 g         1621 t
ORIGIN      
        1 tggaactggt gaccatgaaa accaccggtg acaaaattct gaacaagacc ctggacaaga
       61 tcggcggaaa gggacttttc ttaaaggaac tggatgccgc acttctggaa aaacaggtgg
      121 acatttgtgt ccatagtctg aaagatgtgc cggcgcagga aaatccgctg ctgcccatcg
      181 gagcgtattc cggacgggaa gcgccggcgg atgtgctggt acttccgaaa ggacagaacc
      241 ggcgggatcc ggcgctgcct gtgggatgtg ccggggcaag gcgtgccatg cagtttcaga
      301 aactgttccc ggatgcggtc ataaagccgg tgcgtggcaa tgttctgaca cggctggaaa
      361 aactggatcg tggcgaatat ggagcgctgc tgctggcgga ggctggtctg atccggctgg
      421 gactggcaga gcggatctcc tatcgctttt caccgaatga aatggtgccg gctgccggac
      481 agggcatcat ggcgatccag acccgtgcag gggaatatgc cgggctgctg cggcagctga
      541 accatgaaga tgctgcattc tgtgccggaa cggaacggcg cttgtccgga ctgctgggcg
      601 gtgactgttc tgcaccggtg gggtgctatg caaagctgca cggggacgat ttcacgctct
      661 atggcttttc cggcattgac ggaacgccga gattcgataa agtacaaggc aaacgagagg
      721 acgccgaggc gctggtacag gcgttggcgg cgcagttgag taagaaggtg tccatatgaa
      781 tttgatcaat cgtccacgcc ggctgcgaaa cggcgcactg ctgcgcagca tggtgcggga
      841 aacccgtatt tctgcggata cgctgatcta tcccatgttt gtacaggacg gggagaatat
      901 ccgggaagag atcccgtcta tgcccggaca gtatcgctgg agtctggatc gtgtggatga
      961 gcttttgcag caggtgacgg attccggggt aaagagcgtt ctactcttcg gaatccctgc
     1021 ggaaaaggat gaaatcggca gcagcgcatg gcagccggac ggcattgtac agcgtgcagt
     1081 gcatcatatc aaggcggcat atccgcagct gtatgtcatt actgatgttt gtatgtgtga
     1141 gtatacctct cacggacact gcggcattct ctgcggtcac gatgtggata acgacaagac
     1201 gctggaggtt ctggcgaaaa cggcgctgtc ccatgtacag gcaggagcgg atatggttgc
     1261 accgtccgat atgatggacg gcagagtggc acgcatccgg caggtgctgg acgaaaacgg
     1321 ctatgtgaac acgcccatca tgtcctattc cgtcaagtat gcctcggcat tctatggccc
     1381 gttccgggat gcggcaggtt ctgcgccggc gttcggcgat cggaaaagct atcagatgga
     1441 tccccacaat gcacgggagg cgctgctgga ggcagaactg gacgtgcagg agggcgcaga
     1501 tattttgatg gtaaagccgg gcatggcgta tctggatgtg ctgcggtcgc tgaaggaaaa
     1561 gtttcaccag cccgtggcgc tgtacagcgt cagtggtgag tatgccatga tccaggcggc
     1621 agcggctgcc ggctatatcg accgggaggc tgtgatctgt gagtcggctg tttgtatgta
     1681 ccgtgccggc gcagacctgc tcatcaccta ttttgctatg gaactggcgc agtatattcg
     1741 ggaagggaga atcggataat ggaacagatg cagatcacac attctgagga actctactcc
     1801 cgtgccaaag aactgatgcc cggcggcgtc aattcgccgg tacgtgcatt tcagtccgtg
     1861 ggcggaacgc cgagatttat ccagcgtgcc aaagatcagt acctctacga cgaggacggc
     1921 aacgcttata ttgattatat tggttcctgg ggtccgatga tcctggggca taacccggac
     1981 gtggtgctgg aggcagtacg cagccagctg gaaaacggtc tcagctacgg cgctgctaca
     2041 gcgctggaag tggaaatggc agaactcatt cacgagatcg ttccctgtgt ggaaatgatg
     2101 cgcatggtaa attccggtac tgaggcagtg atgagtgcgg ttcgtgctgc acggggcttt
     2161 accggacggg acaagatcgt caagttcgag ggctgctatc acggtcatgc agacgccatg
     2221 ctggtgaaag ccggttccgg ggtcatgact gccggaatcc ccgattccag cggcgtgccg
     2281 aagggctgca cccaggacac gctgtctgcg gtgtacaatg acttggacag cgtggaacgg
     2341 ttgttccggc agtatccgca ggagatcgcc tgcgtgatcg tagaaccggt ggcagccaat
     2401 atgggtgttg tcgcaccgga aaagggcttt ttgcagggac tgcggcagct gtgtgaccag
     2461 tacggcgcac ttctgatttt tgatgaagtc attaccggtt tccggttaca gctggacggc
     2521 gctgtgggtc actttggcat tacgccggat atggtgacct acggcaagat catcggcggc
     2581 ggtatgcctg ttggcgctta cggcggcaga cgggagatca tgcagctggt ggcaccgctg
     2641 gggtctgtgt accaggcagg tacgctcagc ggcaatccgg tggcaatgcg tgccggactg
     2701 acacagctgc gctatttgca ggcacacccg gaggtatatc accaggtggg atttttgtct
     2761 caccggctac gcagcggtct ggaggatatc atccgccggc accatgcgcc gtgtactgta
     2821 aacgggatcg gttcgctgtc ctgcctgtac tttacaccgg aaaaggtgcg gaactatgcg
     2881 gatgccaaaa aggcagacac cgcaaagttc cgggagtact ttcacttcat gctggatcgg
     2941 ggcaattatt ttggtcccag ccagtttgag gcaatttttg tatcgaacgc acatacgctg
     3001 cgtgagatag ataaaacgct gggagatgca gacgaatttt tcgctgcaca cagcggaaac
     3061 tgagaacctc tttctgttct cagccgggag ccaacggaga ggggttccga gaggagaggt
     3121 tttcatatga acaaacaaaa tggctctgct ttgcagaacc gcagcaccgg aggcatgacg
     3181 ggatgtcgtg tgctggtgtc tgccctggca gtctgtctgc tgctgtctga tatggcagca
     3241 agcggcaaat tcggtgcatc cgcaatgcac atcatggagg gctatctgcc tgccggatgg
     3301 tgtattgcgt ggggtgcact ctgtattccg ttcctggtgg ctggtgcagt cagcctgaaa
     3361 cggaagatcg cttatcaggg aagagtgaag ttgctgatcg ccctgtgcgc agctttcgtc
     3421 tttatcattt cttccttgaa gatcccgtcg gttaccggta gctgttctca tgccaccggt
     3481 accggactgg gtgcaattct gttcggaccg tccatcatgt ccgtgctggg cattatcgtg
     3541 ctgctgttcc aggcgatcct gctggcacac ggcggtctga ccacactggg cgcaaatact
     3601 ttttccatgg cgattttcgg accgttcgtt tcctacgggc tgttccggct gctgaaaaag
     3661 tgcaaagcgc ctatgggtct ggcagtattc ctgtcggcaa ccatcggaga catggcaacc
     3721 tattgcctga cttcggtaca gctggcggca gcatatcccg gcgataactt ctgggcatcc
     3781 ttcggaaagt ttatcgctgt ctttgcaccg actcaagtcc cgattgccat tgcagaaggc
     3841 attctgactg tcattgtgta caatgtcatc cagaagaact gcgcaaagga gttgaaggaa
     3901 ctgtccggca ttacagtttc caaccccgga cgtggcaagc tgtgggcaag aaatctggtt
     3961 ctggcatttg gcgttgtcct gctggtgacc attccgctga tgctgctggg caacagtgaa
     4021 ttcggcggct cggatgatgc aggctctact gtggtagaag agatcaacgg cggctatacc
     4081 ccgtggtttg aatccttctg ggaaccgcct tcgggtgaga tcgaatctct gctgttctgt
     4141 gtacagtcgg cgatcggcgc aggtatctgc ggctttgtat tgggacgcat cagccgaaag
     4201 ccaaaggcgg aaacggtatc tgcaccgaca gattctgaac catccgaaac ttctgactaa
     4261 cacaggaggc acaagatgaa acgacacaca cacagccata cccacggaga acgcattctg
     4321 atcgatgaga tcgcatatgc ctcggcactg cgccaggttt ctccgatgtg gaaaattttt
     4381 gtgtnnnnnn nnnnatgatc ctgttacagg tctcacacca cggcgagaaa atgctgctgg
     4441 cgattcccgt cggcagctgg tatttcggcg tgacggcaga gagcgtgcat cagttcgggc
     4501 aggtatttct ccagtgtctg gcggcggtga gctgtctgta ctttctgtca acctccaccc
     4561 cgatgacgga gctgtttacg gcgctgcgcc ggatgcacat tccgggattt ctggtggaga
     4621 tgatggaact ggtctatcgg tatattttcg tgctgctgga ggcggcatct cagatacgaa
     4681 atgcacagga gtgccgtctg ggatatgcta cgctgaaaac cggattctat tccaccgggc
     4741 agctgatctc caatctgttt ctccgggcat accggcaggc agaccggaca tataccgcca
     4801 tggaatccag agggtataca gacgaagtgc ggacactggg actggcatat gagacaaagg
     4861 gctggcagaa aggacttgcc gtcgggtaca gcgtattgct gacggcggca gtgctgattt
     4921 gcagaatagg aggcagcaga tggggattat tttagaggca gagcatctga catacggcta
     4981 tgaggaaaag cgtcatgcca ttgaagacct gtccattgca tttgaagagg gtaagacaac
     5041 agcgatcctg ggtgcaaacg gcgccggaaa atctacgctg tttctgcatc tgaacggcat
     5101 tttgcagccg gaacacggcg aggtgcgctt tcgtgggcag ccgatctcct ataagaaagc
     5161 agggctgcgg cagttgcgtt ccaaagtggg aatcgtgttc cagaacccgg atgaccagct
     5221 tttttcggcg tccgtgtacc aggatatttc ctttggtgca gtgaacatgg ggctgccgtc
     5281 agaggaagtg catcggcgtg tgaatgcggt gatggaacaa attggaatca cttatctgaa
     5341 agaccgtccc actcattccc tcagttttgg gcagaaaaag cgtgtggcat tcgccggcat
     5401 tatggtaatg cagccggaag tgattattct ggacgaaccc actgccgggc tggatccggt
     5461 gggggtcagc gagctgatgc acctgctgca ggacgtctgt cggcagcaga acacaacaat
     5521 catcttatcc acccacgaca ttgatgttgt gccgatctat gcagatgtga tcgacgtgat
     5581 ggatcagggt catgtgatcg cacacggcac gccggaagag atctttgcac agccggaact
     5641 gctccggtcg catcatctgc ggctgccgag aatttcacat ttgctggaga ttctgcaaaa
     5701 agaggacggc tgggatgtgg atgcttcggt atctaccatt tccggcgcaa gaaaagaact
     5761 gctgcatcac gcagcaaact gaggagaact atccatgaaa acaggacgtg tatttctggt
     5821 gggcgccggt ccgggagacc cagcgctgct gacggaacag ggaagaaaat gcctggagca
     5881 ggcggatgcc gtagtctatg atgcactggc gtgttcgtct gtgctgaatc tgacaaagcc
     5941 ggactgttcg ctgatttttg ccggaaaaat ggctgggaat cactacaaaa agcagagtga
     6001 gacaaaccgg ctgctggtgg aacttgcaga gcagggaaag caggtggtgc ggctcaaagg
     6061 cggtgatccg tttgtctttg gacgtggcgg agaagaggcg caggtattgc aggaaaacgg
     6121 cattccgttt tccattgtgc cgggtgtgtc ctcctgctac agcgtaccgg catatgccgg
     6181 catccctgtg acgcaccggg cgtatgcgcc tgcatttcat gttatcacgg gacaccgcaa
     6241 gacggaacag cacgctgaac tggactatgc cacgctggca aagctggacg ggacactgat
     6301 atttctgatg agtctcagca acctgccgca gatcgccgca gcgttgatac agaacggaaa
     6361 gccatcggag acccctgcgg cagtgatcca ggagggaacg acagcgcacc agcgtgtcgt
     6421 gactgcatct ctggaaaaca ttgcggaaga ggtcagacgg cagggcatcc atacaccggc
     6481 aatgacggtg atcggcgatg tggtcggtct gcgggaacag ctgcaatggt atggaaacag
     6541 tatcttatcc gggaaaagcg ttctgctgac cggaacgccg gaatacaacc gaaaagccgc
     6601 agaacgcctg cggcagtatg gtgcggcatc tgcggagatc agtctgatct atccgtcact
     6661 gctgcatccg gacaaactga aacagctgtc ctgggaaagc tatacctggg cggtgatgac
     6721 cagcgccaac ggcgtggaga tgctctttgc cgccatgcgg caggctgggg tggatctgcg
     6781 cagtcttttg cacctgcggt tcgccgctat cggaaaaggc acagcacagg cgctggcaga
     6841 ccacggcatt ttcgtggact gcgtgccgga gcatttcgac agccgcagcc tggcagaggc
     6901 gctgattccg gaactgacgg aaaatgacag ggttttgctg ctgcgtgcag aaaacggttc
     6961 tgttctgctg ccgcagctgc tggaacaggc aggaaaggcg ttcgacaatg tgccgctgta
     7021 cacggtgaaa accgaccggc gaaagcagga actgctgcgc caggaactgg aacgcacaga
     7081 ctatgtgttc cttgccagtg catctgcggc aagagcgttt gcggaaatga cgggagagat
     7141 ctcatattcc gcagaagtcg ttgcaatcgg agacgccacg gcgaaagctg ccgcagtagc
     7201 tgagatttcg gtaacgcata ttgcggcaca ggcggatatt gacggaatga tacaatgtat
     7261 atgcaaagaa gaggtgtaaa aaatgtatcc gatctcactg aatttacaag ggaaaaagtg
     7321 tattgttttc ggggcaggaa aggtggcgca gcgccgtgtc tggggtctgc tggatgccgg
     7381 cgcagaagtg actgtcgctg caccggagaa aatgccgg
//
LOCUS       DAWENO010000100         7398 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4095_length_7398_cov_13.047120, whole genome shotgun sequence.
ACCESSION   DAWENO010000100 DAWENO010000000
VERSION     DAWENO010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4095_length_7398_cov_13.047120"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    660..669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1616 a         2193 c         1766 g         1813 t
ORIGIN      
        1 ctcgctacct ccaccttggc aaggtggcgc tctaccagat gagctaaatc cgcatccgaa
       61 acgtattgca gtaacacgtt tcatggtgcc tccgatcgga atcgaaccaa tgacacgggg
      121 attttcagtc ccctgctcta ccgactgagc tacagaggca tcacagcaag gctgcattgc
      181 tggcgatctg gatgggagtc gaacccacga cctctgccgt gacagggcag cgttctaacc
      241 aactgaacta ccagaccact ggtggaaact atagggctcg aacctatgac cctctgcttg
      301 taaggcagat gctctcccag ctgagctaag cttccacttg gtgtcttttc ggtgtgtttt
      361 gtgttttcct caccggcgac tattacagta tatcacattg caagggtttt gtcaagcgtt
      421 tttttcaaaa aaatcgaaaa aaagtgcagt ttgtgacatc tgtatagttt caacattgta
      481 taatatgata tactatacag aaaagacagc caaaatcgct aatttctccc attttctccg
      541 ctgcgccgga aaagccgcag tacttccagc cgcagcgcac cgtgtgccgg cgcagaaaga
      601 tccgggtcct gctccagaat tttttttgcc gtttcctgca cttcgtggag cagctgtgtn
      661 nnnnnnnnnc tgtgtgtcat gagacaggtc tgcgatctgt aacggcggta gaccgtgctg
      721 gcgctgtccg aagaagtcac ctggtccacg cagttccagg tctgcctcgg cgatctgaaa
      781 gccatcactg gtgcggctca tgatctgcat tcgggcgcgg cactcctccg tcacatgatc
      841 tgtcattaaa atgcagtagc tctgtgcgct gccacgcccc actctaccac gcagctggtg
      901 cagctgcgac aatccaaacc gctccgcatt ctcgatcagc agaatagtcg cattcggcac
      961 atccacgccc acctcgatca ccgtggtgct gaccagcaga tccagttcgt ggttccggaa
     1021 cgccgacatg accgcatcct tctccgccgc cttcattctg ccgtggagca gtcccacccg
     1081 atactgtgca aacgcaccgt tctgaatgtt ctccgcatag ctggacaccg cctgcaattc
     1141 gctttcgtcc cggtcgtcga tcatgggaca gacaatatag ccctggtgtc cctgctccag
     1201 ctgctgccgg ataaaatgat aggcacgctg gcgcagcttt cccgtgaccg caaacgtctg
     1261 caccggcagt ctgccgtgag gcaattcttt cagcacagag atatccaggt cgccgtagat
     1321 catcagtgcc agtgtccgtg gaatcggcgt tgccgacatg accatcatgt ggggacagcc
     1381 gcctttatct gccagaagtg tccgatgtgc cacgccgcac cgatgctgtt cgtcggtaat
     1441 gaccagcgcc agtcgtgcaa aggtgacctc tttctgaaaa atggcgtgcg ttcccaccac
     1501 cagcgccatg ctgccgtcag aaagcgcctc atagatctcc cggcgctcct tggcacggac
     1561 agacccggtc agcagtccca ccgaaacgcc cagcggttcc agaaactgct gcatggtgtg
     1621 ataatgctgc accgccagaa tttccgtcgg cgccatcaat gcgcactgca tcccgttctg
     1681 cgccgcaaaa tagcacgccg ccgccgcaac agccgtcttg ccgctgccca cgtcgccctg
     1741 caaaagccgg ttcataggga catcgccgca aaggtctgcc gtgatctctt ccacggcgtg
     1801 tttctgcccg tctgtcatgg tgaacggcag tgccgcataa tagggtgaca gatctgtgtc
     1861 ccggcgcatg ggatacgccg atttcgtccg gttgtccgcc cggcgcagca gcatccccag
     1921 ctgcaattgc agcaactcat caaatgccag ccgccgccgt gccgtctcca tcaacgccgg
     1981 atttgacgga aaatgcacct gctccagcgc cgttcccagg ggcagcagac catgttccag
     2041 ccgcatctgc tctggcatcc actcgaaagg ctgtgccttc ataagttcca gcgcctgttt
     2101 caccgcacgc accagcagat tcgccgtaag gccgcgggtc agcggatatg ccggatagat
     2161 cagttccttg ctgttgtctg ccagcacatg aggcgcatga atctcacgcc gcagcagatt
     2221 cccctgtatc tttcccgaca tccggtacca gctccccgga tgcagtgcct ggaaaccgta
     2281 ctggttgttg tacatgacga ccgtcacatc gcttacgccg tcggttgcca ccgccttgaa
     2341 gatagtcatg cccttgcgga taaactgagg tgccagctta tgcacgatct gcacccggat
     2401 cacgttggtc tctcctaagg atgccgccgc aacaggaacc ggatggctgt agtccagata
     2461 gctccgtggc acatgataca gcaagtcata gggtgtctgt atccccagct ttgccagcgc
     2521 ctcagctcgc tttgcgccaa tgcctttgag attggcgacc ggctgaaaaa gtgcatccat
     2581 atgagaaacc tcctttctgg tgtcgttttt atacggctga cgcttacttc ataccctccg
     2641 gcgcacggag cggacacttt cccgtcagtt catagtgaca gttgcagcac tcgtccccgt
     2701 ctcccagcgt cttggtacgg gaaaagccgt tgcccatgta atgggaaacc atatagtcca
     2761 cagaacagat tcccggcagc attccctcta cgccgtattt ctttgccaca gtgataaatc
     2821 cgcagcgccg gatgtcgatc tcaaagctgt tcgcatctat atcccggtag tcgatcagcc
     2881 agtcgtattc cgaaaagccc ggttttccct gccgctgcaa gtgctgtttc gcctttttcc
     2941 gaaagctgtt cagatagctt ttccctgcca tatgcaggaa aggtttcggc acagaaagca
     3001 gcatcttctc attcatcttc cacatcagat cgtccgcctg cccggcatcc agccccagct
     3061 gacgcataga ccgataccac gccacatagg acggcgcata ggcataatta aaggaaaaaa
     3121 tactgtcccc gatgtccggc agttctgccg ccacctgacg aaacacagcg tccgaatgtt
     3181 cccggaacga tttccaaaac gattttccgt attgcttttg aatataccct ttgctccggc
     3241 acatggtcag ccgataaatg aatttctgca tggttttcgt cctttctctt cggaattgcc
     3301 attgttttta ttataccaca ttttcctgca aaaggcaaca aaaaagcttt cccgtccgaa
     3361 aacaggaaag cttttggttt tttcaaaatg ttttttatag gaacacgcct tacttgttgt
     3421 tgtagccgtc gatcacttcg tcaatgacag aagaccactc atcacgcaga acagcccagg
     3481 aatccttgtc gccgttcagc agtgcagaag tcagattgtt catgacacct cttgtctcat
     3541 aggtgtattc gccgtcgccg tacattctgc tgcccatgcc atagccgaag tcaaataccg
     3601 gtgtgatgtt cttcggatcc ttgtaatcct ggagtgcatc atactgttct tccgtcacat
     3661 aggacttcag cttgccctgt gcattcactt ccggaatgat tgccttttcc ttggctgcat
     3721 ccttgtattc gtcgatcatg gatgccaaac gctcgcactt aatgtacgcc gcaacagctg
     3781 cacccttgtc agagttcttg accagcatct ttgcgccgaa gttgcagttc agatagtact
     3841 cgtcgctgcc cggctgtttc gggaacggca cgatcatcag atcgttttct tctacataac
     3901 cgttatcctc atagtccggt ttcgggtcag ccttgacgtt ggatgcaccc agcgcccagt
     3961 ctgccatgcc gaaaaacagg gtcgagccgt cgtccggcag atagccgtac caggtcgggt
     4021 cgtacagacc caggctcatg atctcttcca tcacgccttc agcctcttcg attgccgggc
     4081 tgctgatatt gttggagaac tttgtcccgt catacttgac aaaggtctca cctgtggact
     4141 gcaccattgc ctgaccgaac cagccgttga tgccgtagcg ggtctcgccg tccttggcgt
     4201 tggagacaaa cgtcttcatc atatccatga aggtatccca gtcccacttg tcctttttgt
     4261 acagctcgta cgggtcatcc agaccgtttt cctcacagag cgaacggtta taagtgatga
     4321 gcagcgggtc agacagacag tacggtacta cgtagtgctt gtccttatac ttgaacattt
     4381 cgataacgtc cgacatatcc gaccagatat catcacccag atccagatag tcgtccagcg
     4441 gctgatactg ctccttggtg acgccgttcg gcacagcatc ccactcatac gggaacatat
     4501 ccaccgggtc accgcccaga atgcggcttg ccagcgtatc gaacttggtg tcagaggtgc
     4561 aggagacata ctccaccttt gcgccgtact tatcctcaaa cagcgacagt gcaacgcttc
     4621 tgtcctggtt gttcttcggg ttgatgtcat agtctgccag ccagtagatg gtctgtccct
     4681 tgatatccgg gtcatccttg accatatcgt ccaccagaga tgcgtctaca gcgacatcat
     4741 ccttttcctt ataagcagat tctgcctccg aagaggatgc gctcttcgag ctgtcagaac
     4801 cgccgcaggc acccagtgcg cccatcattg tcagcgccat cagcgcagcc agcatacgtt
     4861 tcaaagtttt catttccgtg aaagactccc ttccagtttt aatcgtacca aaaaacgatt
     4921 cgttttaacg aatccaacac ttgtattata gcataaaaag cggcacaggt caagcttttc
     4981 agccaggcaa cttgcacaaa tcgtgggttc gttttttgga ctgccgccgg attcgtcgcc
     5041 ggatttttcc cggactctgc acgaatctgc cgcaaagcag ttgcacagcc cggcaattta
     5101 tagtataata gaaaccagac acatcacaca ggaggaaacg tatgaaaaca gtagagatct
     5161 ttaccgacgg agcgtgcagc ggcaatcccg gaccgggcgg ctggggtgcg atcctgcgct
     5221 acggcacagc cgaaaaggaa ctttccggcg gcgaacacga gaccaccaac aaccgcatgg
     5281 agctttccgg cgtgatcgcc gcactctccg ccttaaaaga gccgtgccac gtgctgctca
     5341 ccacggacag caaatacgtg gtggacagcg tcaccaaagg ctgggtctac ggctggcaga
     5401 aacggggctg ggtgaagtcc gacaaaaagc cggcactgaa cgtagacctc tggaaacagc
     5461 tgctgcccct gctggaaaag caccaggtca ccttccactg ggtcaaggga cacgccggac
     5521 atccggaaaa cgaacgctgt gaccagctgg cggtcatgca gcgtgaccgg aatgcgtaag
     5581 agcctgtaaa aacggaaaac cctcactctt tcgagtgagg gttttcctgt attatagggg
     5641 taaaaatgaa aaaatgagat tgtctaaaat aatagattcc agaagtcgat tacttcttct
     5701 ttctggtgat ggtgtatgcg cctgcgccca gagccgcagt tgctaccgca cccagcacga
     5761 ttgctgcaat gccgaagtcg ccggtctgag aagtggtcgc attgtttgtg gaaccattgc
     5821 tgctgccgga gctgctgcca ttggaagaac cgccgttgct gccgcctgct gcggtagtcg
     5881 tagtttcacc tgccgcagtg gtcttgctcg gatctgcggt tgtgccattc ggatccgtgt
     5941 tcttcggtgc ctcagtggta gacgccggat ctgtggttgc agtaccgccg ccggcattga
     6001 tgccgcttac agcgaactga atgttaatgg tctcgcctgc tgcgataccg cccggaatgt
     6061 tcttgtcgat gtcattggtc atctgaactc cgccacccca ttcattcagg atgttcattc
     6121 ttgcagaaga accgtcatct cttgtgcagt atgccttgtc tccaggacca ttgtaagcga
     6181 tctctgtttc agagccgtca aagtgaccta cccagatctt cttgatttcc agtttcagtg
     6241 tgccttctgt cttatagtca ccgccttctg ctacaaaagc ataaacgttg atatctgttt
     6301 ccagagacag gaactctact gtttcagaac cgccgtcttc cggtacagtt gcacttaccg
     6361 tgtacacgcc gtcaccagtg atattggcat tcttatcttc ccagtcacct gcattccaca
     6421 tctggttttt acccatagat gtagcgatcc atgcagtata cggcggctca ctcttgccgt
     6481 ctgcggatac agaaatagaa gctgcaacca tagaaacagt tgccactgct gtagctgcca
     6541 caactgcagc gatcttcttc ataaatttca tcgtaaactc ctcctaattt tcacacaccg
     6601 ccgagcagtg cataccacat tctttctgta ttatagcata tttcaaaaaa actgtctatg
     6661 cacattttgc ctaaatctca aagtgaaatc cgttgatttc gccgaaaacc atacaaaatt
     6721 gcagtctggt tttcgtgctt tttctgcatt atttgatacc attcccggtt ttttccaata
     6781 tttccagcac atcccggagc agttcaagcg gcggttcgtc ctccagtttg acacccagat
     6841 aggacttccc cacactgccg aactgcactc tgccccggta tttctgcgac agcgccccga
     6901 tcatctccgg cgtgggatgt gccagataga acagcatcac gccgccacgc tgattgatct
     6961 ccgtgatgcc cagctgtgcc gccgtgttcc gcagccggga aacggtgata agccccacca
     7021 cttccctggg cggctcgccg tagcggtcga tgagttcgtc cagcaggtcc atttcgtcct
     7081 cgctgttctg cacgctggcg atcttccggt acatatccag ccgctggctc agactctcca
     7141 tatagctttc cggaatatgg gcgctgatct ggatatccac cgtacactct gcggttttct
     7201 ggatcggctc gccctttgcc tctgccacag cctcgcccag cagccgcaga tacatatcgt
     7261 agcccaccga ctccatgtgt ccgtgctgct gtccgcccag cagactgcct gcgccacgta
     7321 tttccagatc acgcatggca atgtgaaagc cgctgccgaa ctgggtgaat tcccgcattg
     7381 ccgccagccg tctcgccg
//
LOCUS       DAWENO010000101         7368 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4120_length_7368_cov_10.954191, whole genome shotgun sequence.
ACCESSION   DAWENO010000101 DAWENO010000000
VERSION     DAWENO010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4120_length_7368_cov_10.954191"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1940 a         1830 c         2075 g         1523 t
ORIGIN      
        1 gaccggggca gaaagtgaac cacacgatct gcagcgatgc cggtgaaaat acccgtgata
       61 cagccggaaa ccagcagaaa cgggaaatag acaatgacct gcaccgactg catcactaca
      121 attgccgcag caatctgtcc cacattgtgc agcacagcgc cgatcatgct gatgaaccag
      181 agatgtttcc ggtgcagcag cctgcacagc agtgccattc ccagaaagca gaggataccg
      241 ccgctgaggg aatagatcat ggaaaccagt gttccggtga acagcgaccc cagcagaatg
      301 cgcatgacca gtacggcgaa cgcatccctc gggcggtaga ccaccagaac gatcagcgtg
      361 atgacattgg caagtcccag cttaacgccg taaatgggga cgggaatggg aatctgtgcc
      421 tcaaccacga agatcgtcag cgccatggta gtaaacatgg caagcatgac cagatgctta
      481 acggacttgg acatcgaaag aaccctcctc gtcagatgca atggaaatgc ggatgtgatt
      541 cggcaggcag acgatgggca ggatgccgtc agagatcgtc ccttgcttga cgcacagatg
      601 atcggggcag cttgcggact gcatggaaat ctgtcccggt tctaccagga ctacgttttc
      661 cgcaccgttt tcgccgtcga tcgtaatggt gtatgccttc tgcaccgtag aaaggtcgat
      721 ctcatataaa agcttgtcct gctgataaat gcgggcgatt ctttcggact gcttgtgtgt
      781 cagcacatac cagctgccga aaatgccgcc gcctaaaagc agtacagcga tgcaaacaaa
      841 aatcagtgtt tttttcatga accaattacc tgtagattat aggaatctat gtggtcggag
      901 gagaccattg tcccatctga ctggataaag acagcctcca cgccgtccag agaattgata
      961 agcgccatgc cgtcctctgc gcccaggaga aatgttgccg ttgaaagggc gtcgcagtcc
     1021 gtaccgctgt cggcaatgat ggtcgtgctg actacatccg attccgacgg agcgccggta
     1081 tacgggtcga gaatgtgatg atagcgcttt ccgttttctt cgaagtattt ctcatagttg
     1141 ccggaggtga tgaccgcttt gtctgtaaca gtgacagatg ccgtaatgct ttccggcgaa
     1201 aacggatccg tgatcgccac cgtccactgg ctgccgtccg gctttgcgcc gatggtcatt
     1261 acgtttccgc ccaggctcat cagcgcagaa gtgacgccct cttcttccag cgtctttttc
     1321 atctcatcgg aaatataccc ctttgccaca gcgccgaagt tcagcgtaac tgcgtcgttg
     1381 gcaaaggaaa gggactggtt gtccgcatga tagctgacat ccttccagcc gtcctgccgc
     1441 agcagtgcct ggatcttgtc ctccgccgga atggcagcat ggtcggtgcc gatgccccac
     1501 aggtcgatca ggtcgcctat ggtgcagtcc agcataccgc cgcttttttc gccgtactcc
     1561 agcgcagcac gcaatacgtt tgccatatcc gcagagatga gaatatccgt gccgactgcc
     1621 gcctttgccg cagtgttcac ctggttgacc tcggaatctg caatggtgtt ggaaaacacc
     1681 tgttccagtt ccttggcacg ggcaaacgcc gcctccacgc cgtcctgggc gtgttctccg
     1741 taggcacgaa ccatgatgta agtccccatg aattcctggg tatcctcata gacggcatcc
     1801 gatgcgctgt tttctgccgc tgcactttcc ggcgcagcag cactcgaaga ttgttctgta
     1861 gagacactgc tcgtacatcc ggtcagacaa aacagcccgg cgagtccggc agctaaaaga
     1921 tacccttttt tcatcagcaa tagaaccttt ccgaagagag cgccgcatcg gagcgccttt
     1981 ttctggtagt tagtattatc taacaaacga caaaaatagt atagcacgct tttgaaatgc
     2041 tgtcaagtac ggtttgaaaa gatttccgca agacaatttg aatcaattgt gaatccaaaa
     2101 aagatgctac catgctttct gtcgtattgc ccaaaaactt ttcttgcaaa ttgaaaaaag
     2161 acgtggtaca atagaaaagc agcaaaattt cacagacgag aggagataaa aattatgatt
     2221 tatacagtga catttaaccc ggcgatcgac tatgtcgttc ataccgatgc catgcaggtg
     2281 ggaatggtga accgttctca cggagaaaag atctattacg gcggaaaggg catcaatgtt
     2341 tccgtgattc tgaaagaact gggaatccca tcccgtgcgc ttggctttgt ggctggcttt
     2401 accggggcag ccatcgaaaa aggcgtacag gagatgggaa ttgccacaga ttttgtgcat
     2461 ctggaaagcg gattttcccg aatcaatgtg aagatcaaat ccggggaaga aacagaactg
     2521 aacggacagg gaccggtgat tgacgatgcg gcggtaaagg cactgttctg gaaactggac
     2581 gatttgcagg acggggatac gatcattctg gcaggcagta ttcccaatac catgccgtcc
     2641 gatatctatg aaaagattct ggcatatctg gacggcagac agattcggac ggtagtggac
     2701 gctaccaatg atctgctgaa gaatgtgctg aaatacaagc cgtttctgat taaacccaac
     2761 aactttgaac tgggagaatt gttcggcgtt acactgcaca gccgggagga gattgcagcg
     2821 tatgccggaa aattgcagga gatgggcgca cggaatgtgc tggtctctat ggcagaggac
     2881 ggcgcactgc tgctggagga aaacggaacc tgccacaact gcggcgtgtg cagcggcaca
     2941 gtgaaaaact ccgtgggcgc aggggattcc atgcttgccg gattccttgc cggactggag
     3001 cagggcgatg cggcatatgc gctgaaactg gggacggctg ccggcggagc gacaacattc
     3061 tcggagggac tggcgaaacg ggaagagatc gaccgacttt tgcagcaact aggggttgtt
     3121 tgaaaaatgt acggattttt caaacaagcc ctaatgtaaa tgatttgtaa acgaagtcta
     3181 gttctgctga atctgaaaaa ctgctgtggt ataatacgac acagcaaaaa cggaaagggg
     3241 tcaaaaacat gaaaatactg catctcagcg acctgcatct gggacggcgg ctctatcacc
     3301 attcgtttct tccgcagcag gaaaaaatgc tggaacaagt tgtaaaaatc gcagtggaac
     3361 agtctgtgca ggcggttctg atcgtcgggg atgtctatga caaaaatgtc cctgcggcag
     3421 aggcagtgac cgtgctggac agctttctga cggcgctgtc tgagaaaaag atcgcagtat
     3481 ttctcatcag cggcaaccac gattccgcag agcggctgca attcgccagc cagctgctgg
     3541 aacaccaggg cgtgtatatt gccgggacgt tttccggtga gatgcagagt tacgatctgg
     3601 aagatgaata cggaaaagtg catctctgcc tgctgccctt tgtgaaacag gcggcgctgt
     3661 cgtcgctgta tccgaaagag aatttccgca gcctgtccga cggcattgcc catgtgatcg
     3721 ggcagacggc gctttcggaa aaggatcgga atctgctgat gtaccacggt tttgtgctgc
     3781 atgacggcac gccgccggag gaatccgact ccgaattgca gctgggcggc acacagctgg
     3841 tagacgcatc tgtatttgat gcgttcgatt acgtggcact ggggcatatc cataagcccc
     3901 agtgggtgcg ttcgggaaag atacgctaca gcggttcgct gatgaagtat tccttttccg
     3961 agacgctgca aaagaagtcg ctgaccctgc tggactggaa ggaaaaggat aacttgacag
     4021 taaccgccat tccgctctgt ccggaaacgg atatgcgtat cattcgtggc aagctggaag
     4081 atcttctgac atatgcacag cccagcgccg actggattcg ggcagaactg acggatgaaa
     4141 ctctggtgcc ctatgccatg gagcggctgc gtgctgtgta tcccaacgtg ctggaactgg
     4201 aatttctttc gcagtcggag cgcatcgctg ccggtaccac agaggctgag gcagtgccgg
     4261 agaagtccct gctggaactg gtggatagtt tttgcagcag tgtctatcag tatgagcttt
     4321 cagagcatga gacagcgcag gcgctgatgc agtcaatctg taaggaggcg gaacagacag
     4381 aatgaaacct ttagaactga ccatgcaggc gttcggcaca tttgccggaa tgtgcaggat
     4441 tgatttcaaa ccttttgaaa agtcggggat tttccttatc acaggagaaa ctggcgccgg
     4501 aaagacaacc attttcgatg cgatatgttt tgccctctac ggaactgcca gcggtgaaaa
     4561 gcgaaagccg ggaatgttcc gcagcgaata tgccaaaccc actcagcaga ctgcggtaca
     4621 gctgcggttt tcctgcggcg aaaagacata tctggtgcag cgtacgccga gacagcaggg
     4681 ttacaaaagc aacggcgaaa tgaaaaagaa cctggacaat gagagcgcct ttctgtggct
     4741 gtgtcccggc gaggaacagg acaacgtgct ggtctgcgag ggtgcagaac gggtcaaccg
     4801 ggagatcatc tcactgaccg ggattgacgg cgaccagttc cgccagatcg tcatgatcgc
     4861 acagggcgag tttcagaagt tcctgctgga ggattccaag aaaaaagggg agattctgcg
     4921 ccagctgttc catacacaga actgcgaaaa aatccagaag atcctgaaac tccggcttgc
     4981 ggcgcagaaa cagcgtgtga cagagcagga gacacggatt ctcacgctgc tgcaccaggc
     5041 aaagccggcg gatgcgtttc agcagtcgct gtatgcagag cgtctggaac gctccggcgc
     5101 aggcgctgcg gaaaatctct gcgaaatgct ggaaacggct gcggcagaac ttcgggagcg
     5161 tgcggatgcc agagagaaac agctccaggt gcagcaaaag gcattggaac agctgctttt
     5221 ggaactggaa cagggaaaac agcacaatgc cgttttgcag catttgcagc aggcagtgca
     5281 gcttttggcg caggagcagg cacgcttaaa aatgtgcgac acagcggctc gtacggcggc
     5341 gcaaaacgca gaaaagctgc cggaattgca ggaaaaagcg acactgctcc agcatagtct
     5401 cccagcatat gaaaaggcgg cacagctttc cgcacaggaa tctgccctgc aaaaaaagac
     5461 agccctgcaa aagcagctct gcgcaaaagc aaagctggaa tgggaacaga ccgacaccag
     5521 ctgcaagcat ctttctctgg aactggaaac ctatgccgac acagcggtag accataccag
     5581 actgacccat cagatcgaaa atgataccaa tcggcagaac gcagtgctga ccttacagca
     5641 gatgtttgcg gagtatgcgg cggcacaggc ggttcgggac aagcagaaac agcaggcaca
     5701 gcaggcggaa gaacgctatt tcacccggga aaagccggcg tacgaccaga tagaacggca
     5761 gttctttcag agcatggcag gcaatttggc acgccgtctg gaaccacaaa agccctgtcc
     5821 ggtgtgcggc gcattggagc atccccatcc ggcagcctgc acagaagata cggtcacaga
     5881 agagcagttc caaaaagcga gggaatccca gcagcgtgcg ctgcaaaacc tggagaccca
     5941 gaaacacgaa acggcacgcc gggaggaaca ttgccggatg ctggaatctc agcggcaaaa
     6001 gcagctggaa cagtgcggca tggcagcgac ggcggatgca gaagaagtcc gtcagctgct
     6061 ggaacagctg caaatgtcga ttcgggaaaa tcagcagaaa ctggctgcgc tggaaaatcg
     6121 gctgaacgag gaaaagcgga aaagggctgt gctgaaacag cagcaggaac gtctgcccgg
     6181 attgcagacg gcatacgaaa acagccgtgc tgcactagaa cagctggaac aggaatacgc
     6241 cggctgtgcg gcggagcgaa aacagtgcca gagcggtctg ttgtatgcgt cgctgtctga
     6301 ggcggaaaag cagtggaagt tgttgcaaaa caacattgcc cgtctggaaa cggcgtatca
     6361 gactgcggcg gatgccctgc aaaagcagaa agaaaccgtg gcgtcccagc aaaagatcgt
     6421 gcagcatttg caggaaacgg cgcaggatgt tcccggctat gacgttgccg cagcagaaca
     6481 gcggctggca gccatgcgca gtgaaaaggc gcagacggaa aaggcgtttc agctggaaca
     6541 acagcagcaa cagcagtgca gccagacggc aaaggcagtg cggaaagcgc atagcacgct
     6601 gcaaaaagac tcagaacagt gtctgatgat ggaacagctg gacaaaatgc tgggcggtca
     6661 gcaggggaaa cgggaaaaga tctcctttga gcgctatgtg caggcgtatt atttcagtcg
     6721 ggtgctgcaa aacgccaatc tgaaattgta ccagctttcc ggcggcagat accagttccg
     6781 gcggcggcag gaggaaacag acggcagaat cgcatctggt ctgaatctgg acgtgctgga
     6841 tcagtacacc ggaaagactc gttttgtaga aacgctttct ggcggcgaaa cattcctggc
     6901 atcgcttgca ctggcacttg gactttcaga caccgtgcag cagcagagcg gcagcatccg
     6961 gatggatgct atgttcattg atgagggctt cggctcgctg gacagcactg ccctggacaa
     7021 tgcggtacag gtattgcagc agctttccga ccaccagcgt ctgatcggca ttatctctca
     7081 cgtttcagag ctgacagagc ggttcgatgc acagctgcaa gtccagaagg gcgaaaacgg
     7141 cagtcacatc acctgtcagt acgcttaatc atttatcaca gaatgctctc ccgtggaatc
     7201 tggcacggga gagcatttgc atttttgcac aacttttcgt gcggaatgtt tccgaaaatt
     7261 gtttgcaatt tttctgtatt tccaatcatc ttccttgaca acttgttcca gttatggtat
     7321 actaactagg gttaggactt tctaactttt tttcacgtta gatttaca
//
LOCUS       DAWENO010000102         7313 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4153_length_7313_cov_9.120143, whole genome shotgun sequence.
ACCESSION   DAWENO010000102 DAWENO010000000
VERSION     DAWENO010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4153_length_7313_cov_9.120143"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2736..2745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3342..3351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1540 a         2168 c         1796 g         1789 t
ORIGIN      
        1 cacgatagca ccggcactcc attgccgttg caagctcttc tgcacggtga aaagaggaca
       61 ccagcagcgg aaccagaatc ggcaccagtg ccttggcacg ctgcaccaga ttcccctcac
      121 taaagcttgc acctctcgcc ttctgggctg tcatgatctt gtccgtttct tccagcagcg
      181 tcggaataaa gcggagggaa atcgtcatca tcattgcaaa ggtatgcacc ggaacgtgca
      241 gctttttcaa cggcgacagc agacgctcaa tggcgtctgt caaggcaatg ggcgatgtgg
      301 tataagtcag cagagatgaa ccggcaagca gcacgatgat gcgtgtcacc atcagtattg
      361 ccgtccgcac gccgccttcg gtcagtttca gaaaatgcca tttccagtaa acatcgccgc
      421 tgtctaccat gagcagatcc agcactgcag taatcatcag aaccggaatc agcggcttga
      481 tgcttttcca gatcagcctg acgggaattt tggaaaacga cagcgccagg atgcagaata
      541 caacgccgaa caagatcgaa tacagatgct gtcccataaa cagcattgca ataaacagca
      601 gcgtcatgat gatcttgaac cgtggatcca tgcgatgcac cggactggag cctgggaaat
      661 attgtccgat ggtaatgtct ttcagcattg tcactgttcc cctttcttcc ggtaccatgc
      721 gatcagctgt tctttcgcct gctctaccgt gaaaatatct tccgtcagcg ggattccctt
      781 tgcccggagc gcattgcaga cacgggtgat ctgcggcaca gccagtccga tcgcctggag
      841 ttcctgtgaa cggcgaaata cgttttctac cgtaccgtag cagaacacct ttccggcgtt
      901 cattaccaga atcttctgtg cgtggcgtgc cacatcttcc atgctgtgag agaccagcag
      961 aatcgttgct ccggtctgtc ggcggtacgc cttgatctcc tccaggatct cttccctgcc
     1021 acggggatcc aacccggcgg tcggctcgtc caggatcaat acttccggcc gcatcgccat
     1081 aacaccggcg atcgccacac ggcgtttctg accgccggac agcaaaaagg gcgactgctc
     1141 cagaatgtca tccgacaagc cgacaatggc agccgtttcc tttacccgtg cctggacttc
     1201 ttcctccgcc agtcccatat ttcggggacc gaatgcaata tcctttgcta cggtctcctc
     1261 aaagatctgg tattccggat actggaacac cagtccgacc cgaaaccgca cctggcgcat
     1321 ctgcgccttt tctgcccaga tatcctttcc gtccagaagg atgcttccct cagtcggttt
     1381 cagcagtccg ttcagatgct gtatcaatgt ggacttacca gatccagtat gcccgatgat
     1441 acccacaaaa gagccggact ctatttccag atccacatgg tcaaccgctg ttttttcaaa
     1501 aggagtgccg ataccatagc ggtatgtcag tcctttggtc tgtatcgttg ccaactgccc
     1561 acctcatttc acgccatgca cttgagaatt gcatccacac acgcatcttc gctgataata
     1621 tgaccatcca aaggcatacc ggctttttgg agttcataca ccagttctgt cacctgcggc
     1681 acatccagac ccaccgtttt catttcttcc acatgagaaa agacctctgc cggcgtatca
     1741 tccagcaaga tgtttccgtc atccatgaca ataacacggt cagcctgtgc ggcctcttcc
     1801 atataatgcg taatcagaat tacggtaatg ccgtaattcc ggttcagcaa ccgaacggtt
     1861 ttcatcactt ccatgcgccc cttagggtca agcattgccg tcggctcgtc cagaacgata
     1921 caggacggac gcatggcaat gatgccggca atggcgatac gctgtttttg tccgccggac
     1981 agctgattcg gtgcgtgcat ctggtattca tacattccca ctgctttcag agcatcgtct
     2041 acccgttccc gaatcgtttc cggcggcagc cccagatttt ccggtccgaa cgccacatct
     2101 tcctccacga tcgttgcaac gatctggttg tccggatttt gaaacaccat gcccacgtgc
     2161 tgccggatgt cgtacagctt ttcttcatcg caagtgtcca taccagagac ccacatcttg
     2221 ccgccggtgg gcagcagaat tgcgttcata tgctttgcca gcgtggactt tccgcagcca
     2281 ttatgcccca ggaccgccac aaaagaaccg ggctgaaaag tcagcgacag atctttcaga
     2341 atctccggtg agacggcttt cccctcttca tctgtatagt gaaatgacag gtgttctgcc
     2401 atgatctggt tttccatggt atttctcctt tgcgacatta ttttcgccgc attgcaagca
     2461 gcagaatcac aacaagtacg acaacgccgg cggcaaacag cccatacggc gcaagaaaac
     2521 gaaacatttc ccacacaccc tccggcggca ttccgtagac atagcggcga tccaggtact
     2581 gaatcccggc agcaaatccg tctgctgcca gggcgcaaat gcccaggagc agaacaatcc
     2641 attgttcccg atgcacgtta tcgtttccag atggcagttg agccgcaggg cagcaccatg
     2701 ccgccgcggg ttatgggcag gcccacctcg tcggannnnn nnnnngcacc atgccgcttt
     2761 cttcgaccgg cagaccaatc tcgtctgcgg tcacatcgcc gccgaatttc ggctttaaca
     2821 cgctgcccag cagatacccc attaccgccg gagaaagtcc ggtggtatag ctgttgagca
     2881 ggaagaacag cggatcatcc gaaagcacgc cggaacagag ttctaccaga tcgtagatgt
     2941 tctcttccag tttccagact tcgccgcctg gtcctctgcc atagctgggc ggatccataa
     3001 tgacggcatc gtattttctg ccgcggcgga tctctctcgc cacaaacttt tcgcagtcat
     3061 ccacgatcca gcgaatgggc ttttccgtca gaccggacag cgccgcattt tctcttgccc
     3121 actgcaccat gcctttggat gcgtccacat ggcagacaga tgccccggca gaggcacacg
     3181 ccagcgttgc gccgccggta taggaaaaca ggttcagtac agaaatcggt ctgccggcgc
     3241 tgcgaatctg ctccgccatc cagtcccagt tcactgcctg ctccggaaac aatccggtgt
     3301 gcttgaatcc ggtgggctga atcttaaagg tgagtcccag cnnnnnnnnn ntttcccagg
     3361 aagtgggcag ctttttggcg aatgcccagc tgccgccgcc ggaggatgaa cggctgtagt
     3421 gaccgtctgc ctttttccag cccggatgct ttttcggggt tttccagatg atctgcggat
     3481 cgggccgcac cagtgtgaca ccatgccagc tttccagctt ttccagctgg gaggtatcta
     3541 atatacgata ttgtttccaa ccatttgcta ttctcaattt cgggctactc ctttatttgc
     3601 ttatgatgcg gcaaaaattt ttacctgctc tgtgattgcc tgtgcagcga tctcgatctg
     3661 cgctgcatct tttccctcga ccataacacg aataagcggc tctgttccgc tttcccgaac
     3721 caggatgcgt ccgttttcgc ccaggatggc agtgtatttt tcgatcattg cagcgatatc
     3781 ggcgtcgtcc tgccacagac ctttcttctg cggctgaatg gtgacattat acagcacctg
     3841 cgggaatgtc tccatcaggc ttgccacttc gctcatcttt ttgccggact tcttcagaat
     3901 ctccaggagc ttgatgccgg aaagctcgcc gtcgccagta gtcgcctcat ccaggaagat
     3961 gatgtgtccg ctctgttcgc cgccgatgtt atagccgccg gcaagcatct cttccagcac
     4021 gtagcggtcg ccgacctttg ttgccttggc atcgatctga tttgcctttg caaagtggaa
     4081 aaagcccagg ttggtcataa cggtaactac tgctgtattc tttttcagct tgccctcctg
     4141 ctgcagcgcc tgtgcgcaga tcgcaatgat cttatcgccg tctaccagca ctccgttttc
     4201 atcgacagcc agacagcggt ctgcgtcgcc gtcaaatgcc agacccaggt cgcagtcatg
     4261 ctcctttaca tatgccatga ggttttccat gtgggtagaa ccgcagtttt tgttgatgtt
     4321 tgtgccgtcc ggagatgcgc tgagcatatg tacttctgca cccagacgtg taaagagttc
     4381 ctctgccgtg gtggaagagc tgccgtttgc gcagtccagc gccacacgga tgccggagca
     4441 gtctgttgca acacagcctg ccaggtaaga gatatagtcc tccttggcgt tggtgtactc
     4501 agagatcgca ccgacacgga tatcggactg cagctcgatt ttttccgggt gatccagaat
     4561 cagcgcctcg atctcttctt cggtagcatc cggcagctta tagccggtag ctgcaaacag
     4621 cttgatgccg ttgaactcca cactgttatg ggatgcagag atcataacgc ctgcatctgc
     4681 accgtgtgtc cgtaccagat atgccacagc cggcgtggga actacgccca gcttgactgc
     4741 ctctgccccg acagaacaga ttccggcgca gagggctgcc tccagaatat ccgaagagat
     4801 acgggtgtcc tttccgataa atatcttcgc ccggtgcttt gcctcttttg tcagtaccag
     4861 agccgctgct ctgccgatct gcattgccat ttcacaagtc agttctgtga tggcaactcc
     4921 tcttgcgcca tctgttccaa ataatctgcc catgatctta tttcctttcg ttctgtcaaa
     4981 aatatatttc aggcgatgct gcgcaaaatc cgcttttcag ctttgtgccg catccccatt
     5041 cagctgaact tctcattcag cggaaagtgt catttgtcca tccatgccgc cggcatccgg
     5101 ctgctgatag cccagatgct ggtatgccag cgctgtggca cagcgtcccc gtggtgttct
     5161 cgccaggaat cccagctgca tcagatacgg ctcgcacacg tcctccagcg tcactgtctc
     5221 ttcgcccagg gcggatgcca gggtttccag tcccaccgga ccaccgctgt atcccttgat
     5281 aatcatttcc agcatacggc ggtcgttgct gtccagcccc agttcgtcga tttccagacg
     5341 atccagcgcc atatgtgcga tctcctgtgt gatctcgccg ttgcccatta catctgcaaa
     5401 gtcacgcaca cgcttgagca gacggtttgc aatacgaggt gttcctctgg cacggcttgc
     5461 gatctccatt gcgccgtcta tggagcatgg gatcttcaag agcatggcgc tgcggcgaac
     5521 aatgtccgtc agctgctcag tggtgtagag ttccagccgc aggatcacgc cgaaacggtc
     5581 acggagcgga ctggaaagct gaccggcacg agtggttgcg ccaaccaatg taaacgggtt
     5641 cagatcaaca cgcagagagc gtgccgatgg tccctttccc atgataatat caatggcgtg
     5701 atcctccaga gccgggtaca gaatttcctc gacctgccgg ttcagccggt ggatttcatc
     5761 gataaacagg acgtcatttg gctgaagatt ggtcagtatc gctgccagat cccctgcctt
     5821 ttcaattgcc ggtcctgttg taacacgcag attgacgccc atttcgtggg caatgataga
     5881 ggacagtgtg gtcttgccca gtcccggagg gccatagagc aggacgtgat ccaggggatc
     5941 gccacggcgc tttgccgcct caatgtagac cgccaggttt tctttcacct tatcctgtcc
     6001 gatatattcc acaagcgtct gcgggcgcag ggacacttcc acttcgtggt cttcttccac
     6061 ttcacgtggt gtcaccatcc gctcctcaaa ctgcatatcg ctggtctcga tcatcttgtc
     6121 tcactcctct ctcagaaatt gcgctgtttt ccaaattcct gcaatgtcag ccgaatcagt
     6181 tccgacgccg acagagacgg gtcgagtttt gccacaatgg gtgccacttc actctgggtg
     6241 tagcccagtg tcacaagcgc ctcgattgcc tcgcccacac tgccggcaga ctgagatgcc
     6301 gccgccactt cttccacgcc ggatccggca aacaagccgg cagcgccgcc ggcgatcttg
     6361 tctttcagtt ccatcacaat gcgctgtgcc gactttgccc cgatgccctt ggcacgggtc
     6421 agtgccttgc tgtctccgga ggcgaccgtc agcaggaact gatctggtga cagatcggac
     6481 aaaatggaaa gcgctgcctt tggtccgacg ccggaaacgg aggtcaacag ctgaaagcac
     6541 tgcagctcct gctgagtgga aaagccaaac agttcgactg catcttcccg gacgctcatt
     6601 gcagtatata gaaacacggt gtctcctatg ctgcccagct gtccgagggt ataaagtgtt
     6661 gttcggcagg cataccctac gccgccgcat tctacgacag ccatagtggg ttcctttgcg
     6721 atcaattttc catgcacgct gtatatcata tcgtatctcc tgcctgaatc gttctctttt
     6781 gtaattgtac gccggttgcg cagtgtccat gacaaatggc aagcgccagc gcatccgctg
     6841 cgtcatccgg tttcgggatc tgcgccagtt tcagaatgcg ccgggtcata tccatgacct
     6901 gccgcttttc cgctttgccg tatccgacaa ccgcctgttt cacctgcagc ggtgtgtatt
     6961 catacatcgg gatctggtgc tgttctgcgc aaaggacgat gacgccacga gcctgtgcca
     7021 cgtcgatcac cgtcttttgg tttgacgtga agtacaggcg ttcgatggcg atacagtccg
     7081 gctgaaaccg tttcagcaag taatctaaat cgtcataaat tgccgtcagc cgtgctgcaa
     7141 accgagtccc tgcctctgtg gtgattgcac cgtattccag cggtgtgaaa tccgcaccgt
     7201 gataggatac aatgccgaac cccacaatgg catagccggg gtcgattcct aaaatgcgca
     7261 tcgtatctgc ctcctctgtt tgtgataaga tccggagcag gctttccgta ccg
//
LOCUS       DAWENO010000103         7302 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4160_length_7302_cov_10.048158, whole genome shotgun sequence.
ACCESSION   DAWENO010000103 DAWENO010000000
VERSION     DAWENO010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_7302_cov_10.048158"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1728 a         1861 c         2100 g         1613 t
ORIGIN      
        1 gtatatgatg gacaaagttg ctgcgaaaaa gcattcctcc acagcaaaga tggaactggt
       61 gccgggattc tatgccattt acagcaaggt ctttctcaag atcatgcgta ccaccgattt
      121 gcaggaaagc acgcagaaag ccggcaagaa ctactttgac atcaccatca caaagtgtct
      181 ctggcatacc gcctgtgtgg aaaacggctg tgcggagctg tgcagtttgt tttgcgacgt
      241 ggatgacgtg acctacggcg gcttaaagaa aatcagcttt tcacgaaccc agaccttagg
      301 ctacggcggc agctgctgcg attttcacct ttttaagaag taagctggta cgtttatcgc
      361 aattgcggta ttcccaccaa tcgtcctatt tatgcaaaac gcagcaaaac cacacacatt
      421 ctatatgcaa tctgccgaaa gcaaaaagaa gatgcggaaa acacttgact tttttccgga
      481 agtgtggtat aattcatatc aaacagatag gtaatataga agttaccgga aagggagtca
      541 tatcatgaaa gtttatcaga agatcacaga tctgattggc ggtacaccgc tgctggaact
      601 ggcaaatctg gaggcaaaag aagcactgcc ggcaaaaatc tatggcaaga tggaatattt
      661 caatccggca ggcagcgtaa aggacagaat cgcaaagggc atgatcgacg atgctgaggc
      721 aaaaggtctg ctgaaaccgg gcagcgttat catcgaaccg accagcggta ataccggaat
      781 tggtctggct tccattgcgg ctgccagagg ttatcgtctg attatcgcaa tgccggagac
      841 catgagcgta gagcgtcgca atctgatgaa ggcatacggc gcagagctgg tgctgacaga
      901 gggcgcaaag ggcatgagtg gtgcgatcgc caaggcaaag gaactggcag aggagatccc
      961 gaacagcttt attccgtctc agttcaccaa cccggcaaac ccggaggttc acgaaaagac
     1021 caccggcgtg gagatctggg atgacacaga cggtaccgtc gatatctttg ttgccggcgt
     1081 cggcacaggc ggtacgatca ccggcgtggg caagtatctg aagtccaaga accccaacgt
     1141 gaaggtagtt gcagttgagc cgaagggttc tccggtgctg tccgagggcg tttccggtcc
     1201 gcacaagatc cagggcatcg gggcaggctt tgtgccggaa acactggata ccaaggttta
     1261 cgacgaagtc attgcggttg agaacgaaga tgcgtttgag atcggcagag aaatcgccag
     1321 aacagagggc gtgctggtgg gcatttcttc cggtgcggca gtctgggcag ccatccagct
     1381 ggcaaagcgt ccggaaaaca agggtaagac catcgttgca ctgctgccgg ataccggtga
     1441 gcgttatctg tctaccccga tgttctccta agagaaagca aagcaataga aatttaagcc
     1501 atgaggaaca aagcagttca gtttcctcat ggcttttttg tttgcatttt ccggcggagt
     1561 atggtatact aaaatataaa tgattctggt atggaggtga gactatgacg gcagatatgg
     1621 ggctggggcg aaagctggaa ctgttcctgc tggatctgct ctatcccaac gcctgtccct
     1681 gctgcggaaa gtcgctgccc tggcaggcat acctttgtga gacctgtcag accgaactgc
     1741 tggcatatcc ggcagatgcc ttttgttcac agtgcggaaa gcctgtccat gactgcattt
     1801 gcggcgaagg attggcgtat gaccgggcgg tggcactgac ggtatacgaa ggtgctgccc
     1861 ggaaaggcgt cctttccatg aaatccgcag tcagtctcca gtttggcaga ttctgcggcg
     1921 cagaactggg gaaacgcatc ctggcgaatg atgccttgtc cggctatgat atggttgcgc
     1981 cggttcctat gtccagacgc aagcaattga aacgctgctg caatccggcg gcagtcctgg
     2041 cgaaagaagt tgccgccata acacgtatcc cataccgaaa agaccttttg catgacaacg
     2101 gcacgggacg gcagcagcac accttgtctg cggcacagcg gcgggaaaac accgggcagt
     2161 tttctgctgg gacggtttca ctgaaaggct gccgcatcct gctctgcgat gacgttctca
     2221 ccaccggcag caccatgaac cgatgtgcgg cgctgctgaa agcccaggga gcggcagaag
     2281 tggctgctgt tgccgcaaca acgacacaac tgaaaaagac agaaccagac gactgatttc
     2341 cacccaacat acccagaaag gaacgaaaca tgaatttgat tctactgctg ctttcccttg
     2401 tgatgattgc ctgcattctc tgtaataagc tgaccagcaa aatcggaatc cctatgctgc
     2461 tggcattcct ggcagtggga atgctctgcg gcgtggacgg cattttacat atccggttcg
     2521 acaattatgc catgacggag acgctttgta ccgtggcgct gatcttcatt atcttttacg
     2581 gtggtttcgg cacaaagtgg agcgctgcca aaccggttgc cggaaaatcc gtgctgctgt
     2641 ccacgctggg cgtgtttctg acggcgttcg gtgtcggtgt attctgccgg tttgccctgc
     2701 acaccagctg gatggagggc attcttatgg gtgcagtcct cggctctacg gatgcggcat
     2761 ccgtgttctc cattctccgt tccaaaaagc tgaacctgaa atacgggacg gcatccctgc
     2821 tggaagtcga aagcggcagt aacgacccgg tggcatatct gatgacgacc attcttctgg
     2881 caatgatgac ctcggagatc acgccgggga agatgttgta tctgagagtc gccgagatcg
     2941 gcttcggcgt gggcttcggc gcagtccttg cggtatgcac tgtctttttt ctccggcgct
     3001 ttcgctttga ggtctccggg tttgataccg tttttatggc ggcggtggca atcttgtcct
     3061 atgtgctgcc ggtgctggtg ggcggaaacg gctatctcag cgcctatatc gccggcatca
     3121 ttctgggaaa cgccaagatt cccaacaaga aaaaccaggt gttgtttttc gacggcgtga
     3181 ccagctttat gcagctgatg gtgttcttcc tgctgggact gctctgtaca ccgtcccggc
     3241 tgggcagtgt gatcctgccg gcgcttgcca tcgctgtggt gctgactttc gtagtgcgtc
     3301 cgctggtggt ggcgctgctg ctcacaccgt tccgttcaaa gctttcccag cagctgctgg
     3361 tctcctgggc aggactgcgt ggcgcaactt cggcaatctt tgcgctgacc gcagccgcca
     3421 gcggggcagt gctgcaatac gacctgtttc acctggtatt ctgcgtggtg ctgttttcca
     3481 ttgcactcca gggaacactt ctgccgctgg tctcccgaaa gctgcacatg atcgacgagt
     3541 cagcggacgt tctgaaaaca ttcaacgact atacagaaaa gaaagaactt caactgctgc
     3601 gtctgccgct ggagccggga aacagctgga tcgggcagcc ggtcagtgcg ctgcacctgc
     3661 cgccggatac catgctggcg accatcctcc gggacgggga cagcgttgtg ccacgagggg
     3721 acacggtgct ttgcgaaggt gatgtggcag tactggcggc aggacactgc ggcggcaagc
     3781 tgcaaaatgt ccagctcagt gaaatggaga tcacagccga ccacagctgg aacggcaaga
     3841 ccctggcggc gctgcggctg ccccaggagg aactggtggt gctggtgcgc cggggcaggg
     3901 aatcgctgat tccccagggc gacacggaaa ttcatgccgg cgatgtgctg gtaatgtacg
     3961 gaaagtaaga ggaaatacat attttgaaac aaatcaatct gaaacaagtc aactggatga
     4021 aagcgctgaa aatcgccggc ggcggcgtgg ctgccattct gctggcggag gcgctgggac
     4081 tgcaaaatgc ggcatctgcc ggcattatca cactgctgac cgtgcagaac acacgccggg
     4141 aaacggtgat gagcagcgtc cggcggtttg ccggatttgg catcatgacc ctgctcagcc
     4201 tgccgatcta tcacttctgc ggcactgcgc cctggtcgtt cggcattgtg cttttgctgc
     4261 tcctgctgct ctgctatgcc ttccggatgg acgatgcaac gcccatcaac gcagttatgg
     4321 cgacccacta tatgtttgcc ggcggcgtgt ccatcggtat ggtgggaaac gaaattctgc
     4381 tgctggtaat cggcagcggc atcggtgtct gtctcaactg gtttatgccc aggaatctga
     4441 aaaagatcca cgagatgcag cgccagctgg acgaagaaat ccggggcatc ctggaacgga
     4501 tgtccgtgcg cctgctggag gcggaccata ctggctatga tgacagctgc tttgcacgga
     4561 cagaacgact gtctgaggaa ctgcgccggg aaattgacct gtttttgcag aaccagacct
     4621 ggcaggacga tatttacttt atgcgctaca ctgccatgcg ccgggaacag tgccgggtac
     4681 tgcaagtgat gtaccgccag ctgctgcggc tgaatcagat tccggagcag gcgacacctt
     4741 tgtctgcatt tctgaaagag atcgcacagc attttcatga gggaaatgac tgcacggcac
     4801 ttctggaaca gctggaagag cagtttgcgg cataccgccg ggatgccctg ccggaaaccc
     4861 gtgcggcatt tgaaaaccgg gcgattctct acagcatcct tacagaactg cgctcgttcc
     4921 tggagatcaa gcagcggttt tatctggcgc tgccggaaca ggaacgcaag cagatgtttg
     4981 aacggctgac cagagacatc actccgccgg cacaattgca gtgatattcc cggacagttc
     5041 cgggtaaaca gagactgtcc cggcgtttcg tgcttcgccg gagagcatcc ctgtatctac
     5101 aatgaattcc agaacatttg caccgctttc gcagctgcga tagcggtgcg ttttgtctgt
     5161 gtgctggtct gctttgatat cgcccacccg aacggtaaat tcgctgccgt tttccagcgt
     5221 aacggcaaac cgctcaccgc agccgctttc cagccagcct gtccccatgg cgaccagata
     5281 atcgtccgcc atgcgccgca gtccccggtc atctgtccag gcgttctgct ccatggcgta
     5341 ctggacggag tcggtgtccg tgatggtgcg gtaatccata taggctttga accaggtctc
     5401 gtgttcctgg aaaaacgggt cactttccgg gatggtggag gttactgcga cagttgtttc
     5461 cgactccgtg gcagctgtgg tttcgggcgg tattgtagta gtggctgtgg cggtcagaac
     5521 agtgacttcg gttgccacgg cggcagttgt ctgcgtctgc ggtgactgtg tctccgtagg
     5581 cggtatggac atcgccggag cagtctccgc cggcgtcggc aggggttcca gcggcatcac
     5641 atccaccggg cgaatcgggg aagtttctgg gatggcgtgg gtctggggca gcagaacatt
     5701 gcccagggca gggatctggt gaatggtcag cgtacagaga cacccggcac aaagagatgc
     5761 cagaacttta gaaaattgca gcatgggtca tacctcctgt tcggtgaaac ggcgttgcgt
     5821 atcgtccaag catatgcggc agtgtggaaa agatatgcag tatgacgacg aaatttgtgc
     5881 gtatcgttaa atctaaactg ttcaaatttg tctgatatca cgaaatctac ggatttttcc
     5941 ggaaagcact tgacaaatga agcaaaaatg cgtatactaa aaaaagttaa attccatgag
     6001 caaaagtaag tagccggcaa aacggtagca cagagagtcg gggacggtgt gatcccgatg
     6061 tatacgctgt ctggtgaatg ggtttgtgag aagcgaggcg aacgggcatc tgccccagta
     6121 actgatgccg cagctcctgc gttatcggga gaaggatata tccggtgtat catacggcgt
     6181 atctgagaag agggaaatga acaaggcatc atttctgaaa ttgggtggca acacgtagac
     6241 aacaagtctg acgtcccatg tgaatgggat atcaggcttt ttttatttgc ataaaggagt
     6301 gaacgcatca tgttaagacc gtcattggaa gaggtaaaag cgctggcgaa acagtacaac
     6361 accattccgg tattctatga gttctcggca gacaaccaga cgccgctgaa catttaccgg
     6421 gcattttccc aggagagccg caattcgttt atcctggaaa gtgtcagcaa cggcgtgcag
     6481 tgggaccgct actcgttcgt tggcttttcg ccgaaggaag aaatccggat caccggaaac
     6541 accgctgaaa tccgcagcgg cgagaagagc gagacctttg aggtagcaca tcccattgca
     6601 tttctgaaag aacgcatggc ggcttatcat tctccggtgc tggagggtgc gccgaatttc
     6661 accggcggtc tcattggata tttcggctac gacacggtgc gctatatgga gaaaaagctg
     6721 accaacgtgc cggaagatga catccatatg ccggactgcc acctgttcct gtacgaggaa
     6781 ctgctggcat acgaccactt gaacagcaac ggcattgtga ttctgaacct gcacacagac
     6841 ggtgacctgg gcgcacagta cgccgcagca cagtggagag ccgaggaact ggcagacatc
     6901 gtccgccact ataatccgca gccgcaggaa agacggcatc acggcgaatc ggtggttcgc
     6961 tccaatctga caaaggaaca gtacagccag atggtacggg atgccaagga atatatcaag
     7021 aacggcgata ttttccagat cgtgctttct cagcggttcg agatcagcaa tccgccggac
     7081 agcttttccg tttaccgaaa gctccgtgcg acaaatccgt ctccgtatct ctactatttc
     7141 cacaccccgg attacgatgt ggctggcgca tccccggaga tgctggcaaa ggtgaccaac
     7201 ggcgtgatcc acaatcgtcc cattgccgga acaaagccaa gaggacggac accggaagag
     7261 gacatcgcca acgaaaaggc acttgccgca gaccccaagg aa
//
LOCUS       DAWENO010000104         7280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4170_length_7280_cov_13.256194, whole genome shotgun sequence.
ACCESSION   DAWENO010000104 DAWENO010000000
VERSION     DAWENO010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_7280_cov_13.256194"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1693 a         1936 c         2085 g         1566 t
ORIGIN      
        1 tccagaccga tctcgtggca gaactcaaca gatgtcgggt cgccgccgtg ctcaccgcag
       61 ataccgcagt gcagcttgct gttgaccggc ttgcccaggt cgatagccat cttcatcagc
      121 ttgccaacgc cagtctgatc cagcttagcg aacggatcgc tctcgaagat cttagcgtca
      181 tagtatgcgc ccaggaactt gccagcgtcg tcacgggaga aaccataagt catctgagtc
      241 aggtcgtttg taccgaagca gaagaagtca gcttcctttg cgatctcgtc agcagtcaga
      301 gcagctctcg ggatctcgat catggtacca acttcataag acaggtcaga accagctgca
      361 gcgatctcag cgtcagcagt ttcaacaacg accttcttaa cgaacttcag ttccttgaca
      421 tcgccgatca gcgggatcat gatctccggc ttaacggtcc aatccgggtg gttcttcttt
      481 acgttgattg cagccttgat gatagccttg gtctgcatct cagcgatttc cggataagta
      541 acagccagac ggcagccacg gtgacccatc atcgggttga attcgtgcag agaagcgatg
      601 atagccttga tctgatctac agtcttgccc tgtgcatcag ccagcttctt gatatcttct
      661 tcttcggtcg gtacgaattc gtggagcggc ggatccagga aacggatggt aaccggattg
      721 ccttccagag cctcgtacag agcctcgaag tcgccctgct gcatcggttc gatcttagcc
      781 agagcagcct ttctttcctc aacggtgtca gagcagatca tttcacggaa tgctgcgatt
      841 ctgtcagcct cgaagaacat atgctcggta cggcacagac cgataccttc agcgcccagc
      901 tcacgagcct tcttagcgtc tgtcggagta tcagcgttag tacgaacctt cagagttctg
      961 tacttgtctg cccagctcat gacacggccg aatgtaccag cgatagatgc gtcaacggtc
     1021 gggatgatgc cgtcgtagat gttaccggta gaaccgtcga tagagatgta gtcgccctcg
     1081 tggaaggtct tgccagccag ctcgaacttc ttgttctctt cgtccatctt gatgtcgccg
     1141 cagccggata cacagcaggt acccatacca cgtgcaacaa cggcagcgtg agaagtcata
     1201 ccgccacgaa cagtcaggat accctgagat gccttcatac cggtgatatc ttccggagag
     1261 gtctccagac gaaccaggat aaccttttcg cccttagcgt tccaagcctc tgcgtcgtca
     1321 gcagtgaata cgaccttacc gcaggcagca ccaggagatg cgcccagacc cttgcccatc
     1381 ggagtagcag ccttcagagc ctttgcatcg aactgcgggt gcagcagagt gtccaggttt
     1441 ctcggatcga tcatcagaac tgcttcttct tcggtcttgt gtccttcgtc taccaggtcg
     1501 caagcgatct gcagagctgc ctgagcggtt ctcttaccgt tacggcactg cagcatatac
     1561 agcttcttgt tctcaacagt gaattccata tcctgcatat cgtgatagtg gttttccaga
     1621 gtctcgcaaa ccttgatgaa ttcgtggtaa gcttccggga attcctgttc catctgtgtg
     1681 atcggcatcg gagtacgaac gccggcaaca acgtcttcgc cctgtgcgtt gatcaggaac
     1741 tcgcccatca gcttcttttc gccggtagcc gggtcacggg tgaatgctac accggtacca
     1801 gactcgttgt tcaggttacc gaataccatc ggcattacgt ttactgcggt accccagctg
     1861 tacgggatat cgttgtcacg acggtatacg tttgctctcg gattgtccca gctgcggaat
     1921 acagcctcga tagccagctt cagctgttct accggatcag acgggaagtc tgtgcccagc
     1981 tgcttcttgt attcagcctt gaactgttca gccagagtct tcagatcagc agcagtcaga
     2041 tctacgtcga acttaacgcc cttttcttcc ttcatcttgt cgatgagctg ctcgaagtac
     2101 ttcttgccga cttccataac aacgtcggag aacatctgga tgaaacgtct gtaggagtca
     2161 tatacgaaac gctcgaaagc cggatccggg ttgcccttga tcatagcagc tacaacgtcg
     2221 tcgttcagac caaggttcag gatggtgtcc atcatacccg gcatggatgc acgtgcgccg
     2281 gaacgaacgg aaaccagcag cgggttgttc agatcgccga acttcttgcc gttgatctct
     2341 tccatcttct tgacgccgtc cataacctgt gccatgatct cgtcgctgat cttgcggccg
     2401 tcctcatagt actgtgtgca ggcctcagtt gtgatggtga aaccctgcgg taccggcaga
     2461 ccgatgctgg tcatctcagc caggtttgca cccttaccgc cgaggagatt gcgcatggtc
     2521 atgtcgcctt cgctaaacat ataaacccat ttgtttgcca ttggtaaata cctccaattt
     2581 taatattcga ttctatggcc ggctgcgtga ttgcgtccgg ggaattctgg caaaattccc
     2641 gctttcaacc ggcacattat tattatagca tatttcagat gaaaatacta tacacttttc
     2701 aagaaaaaat tgcgcctttt tttgttgcaa gttatacaaa aatcacaatg cacgattttt
     2761 ttttgaaaaa aggcttgcaa tcctttcaag attttgtcat aatgtaaaaa gggacttttt
     2821 cggatggcgc atccgggaaa gggatactag ccaatttcct tgtccggcgc atatcccgga
     2881 cgagatgtta cattacagga ggaacagaac tatgaatcaa gttgttattc ttgccggcgg
     2941 acacggcaat cgtatgaagg cagcaatccc gaagcctatg ctccaggtgc tggacgttcc
     3001 catgctgggc tgggtcatcc gtgcctgtga gggcgcagga ctgagccgga tctgtgtggt
     3061 caccggctat aaggcgcagt atgtggagct gtatctgggc gaccgttacc gcactgcact
     3121 ccaggcagag cagctgggca cgggtcatgc agtgatgcag gcagaaaagt tcatgaagga
     3181 attcaccgac ggcaacacct tggtgctgtg tggcgatgca ccgtttatgg acagcgagac
     3241 cattgccaat gcgctgaaac tgcatgaggc acaggagaac gctgtcacag tcatcacagc
     3301 gaatctggaa cagcccaagg gctacggcag aatcctccgc acggagaccg gcattgcggc
     3361 tatcgtagag gaaaaggatg ccaccgaggc acagcgtgag atccacgagg taaattccgg
     3421 cgcatactgg ttcaaaacca aggcactgct ggaactgctg ggcggtctgt cccagtccaa
     3481 tgcgcagggc gaatactatc tgaccgacac cgttggtctg gcgctggagc gtggctggaa
     3541 ggcaagcgca taccgcagcg agaacccgga tgtgatcctg ggcgcaaaca ccagaaaaga
     3601 cctgctgcat ctgaacaaag tggcacagga gcgtgtgctg gataagcaca tggaaaacgg
     3661 cgtaaacttc gtcagcaccg acggcatcat catcggaaat gatgtggaga tcggcgcaga
     3721 gactaccatt ctgccgggta ctattttaag aggaaaaacc aagatcggcg caaagtgtac
     3781 cattggcccg aactgcctca tcgaggactg caagatcggc gaccatgtca cactgaacta
     3841 tgtccaggcg tttgagtcgg agatcgaaga tgcggtcaag gctggtccgt tcgttcatat
     3901 ccgtccgaac tcgcacctca agaacggcgt caagatcggt gacttcgtag aggtcaagaa
     3961 ctccaccgta ggcgagcaga ccaccatcgc acacctcacc tatgtgggcg acagcgatgt
     4021 gggcaagaag gtaaacttcg gctgcggtac cgttacagta aactatgacg gtctgaaaaa
     4081 gcagcgctgt gtcatcggcg acaactgctt tatcggctgc aacaccaatc tgattgctcc
     4141 ggtggaactg ggtaagggtg catataccgc cgcaggttct accgtcacca aggacgtgcc
     4201 ggattatgcc ctggctgtgg agcgtgcaga cctcaaggtc aaggaaggct acagcctgaa
     4261 aaagctgaag agcaagctgt tcccggagga cggcgcaaag aagtaaaagt ccatccgtgg
     4321 gctggtgcgg acaacttttt gaaatacgaa aggattcatg cggcatcggg gcatttccgg
     4381 tgccgcatga aacaggtttg agagaaaatg ggtatttttg atttattcaa gcagatcgag
     4441 tcggggtctg ccgggaatct gcacggcaag ctgacctgga tcgttgccgg gctgggcaat
     4501 cctggtctgg aatatgagaa cacccggcac aacgccggat ttctgaccat ggaagaactg
     4561 gcgaaacagt gcggcgcaaa gctggatcag atgaagttca agtcggactg cggcgaggcg
     4621 atgctgggcg aggtgcgctg tctgctgatg aaaccgacca cttatatgaa tctcagcggc
     4681 gatgccattg cggcggcggc gaatttctac aagataccgc cggagcaggt gttggtcatt
     4741 tatgatgaca tctctctgcc gccgggaaag ctgcgcctgc gccgaaaggg cagtgccggc
     4801 ggtcacaacg gcatcaagag cattatcgcc cagctgggaa cagaggagtt tccacggatc
     4861 cgggtgggtg tcggcgcaaa gcccaatccc cagtatgatc tggcggactg ggtgctgtcg
     4921 aagttcagcg aggaagatat gaaggcactc cagccggcgc tggaacacgc agcggatgca
     4981 gcgaagaaaa tcgtcagcgg cgacatgaac ggcgccatga atctgtacag tcactaagca
     5041 acagcagagc aaaagcaaca cggaaaggag acagccatgc agttttttgc agctacattt
     5101 gaacagctga cagcataccg ggaactggtg cggtcacagc aggaaggagt cacgccggtt
     5161 tcgttcaccg gtgtgtcgca gatccacaaa gcgcatctgc tgacggcgct gtgccgggaa
     5221 aatgcaccgg tgctggtggt cactgccgag gagacagagg cacggcggct ctgcgatgac
     5281 atcaacgcca tgctggatga aacggcggca tggatgtttc cggcaaagga ggtggtgctg
     5341 acgccggtag agggcatcac caccgcctat gaacacgctc gtatcgctgc actgacggcg
     5401 ctccggacgg gacagtgccg ggtggtcgcc gccagcattg aggcactttt gcagccgacc
     5461 ctttcaccgg aacagctgga cgactccacg attttgctgc gccgggacgg cacggcagat
     5521 ctgggtgaac tcatccgccg gctctctgcc tgcggctatg tccgtgggga gagcgtttcc
     5581 ggacagggac agttttccgt ccggggcgat atcctggatg tgttcccggt acagctgaat
     5641 gcgccggtgc gtattgagtt ctgggatgat accatcgaca ccatggtaca ttttgacccg
     5701 gagacacagc gccggacgga tgctgttgac gaagtgcgca ttccgccggc aagggagacc
     5761 ctgtgcgacc cggcagcgct ggcggaaaag atccggaaac taggaaaatc cctgcgcagc
     5821 cgcagggcag acctggcacg ggaaaagctg tatgccgatg ccgaccagct ggacgccggg
     5881 atgcttccgg tgcatctgga caaatatgcg ccgctgctcc gggaaaagcc gtccatgctc
     5941 tatgatttcg gcatcgggac ggtctgctgg agcgaatacg gcgcaatcgt ggagagccag
     6001 cgtggactga tggcgcagta ccaggaggac tgcaagctgc tgctggaaga gggcagcctt
     6061 tgccgtgggc tggagggcta ctatcagaat cccacggagg ttgccgtaca gctggacaaa
     6121 cgctttcagg tgtatctcag caactttttg cagggcggcg aacacgtgga atttcagaaa
     6181 ctgctcagcg tccaggcggt gcagaatgcg ccctggggcg gtgagctgcg tcagctgaca
     6241 gaggatctga aagcctactg tgagcagggc tactgcacca tgctctatgc cggcagtgaa
     6301 aaaacgctgc ctattttgca gcaggatctc cgggaaagcg gcatctgctg cgaactggcg
     6361 aagccggaca gcatctggaa agacggcacg gtctatctcc gcaccggcag cctttccggt
     6421 ggatttgcct atccggagat caagacggcg ctgatttccc aggcgagaac gctgcgctcc
     6481 ggtacacgcc gccgaaagac acatcaccgt ggggaagaga tacgcaccct ggcagacatt
     6541 acgccgggag atctggtggt acacgctctg cacggcatcg ggcgctttct gggcatccgc
     6601 aagctggaac tggagggcgt gactaaggat tatatcacca tccagtatgc cggtaaggaa
     6661 aatctgtatg tgccggtcac gcagctggat atggtgtcca agtatatcgg accccgtgag
     6721 gattccaacg taaagctgag ccggctgtcc tcgccggagt ggcagaagac ccggaacaac
     6781 gtgcgccgtg cggtgcggga tatggcagac gaactgctgg cgctctatgc caagcgggaa
     6841 aagaccaagg gctttgcgtt ctatccggac gaccaggaac agcgtgactt tgaggcacgt
     6901 tttccctatg tggagacgga ggatcagatg gcgtgcattg atgagatcaa gcacgacatg
     6961 gaacgagaac gccccatgga tcggctgctc tgcggcgacg tgggattcgg caagacagag
     7021 gtggcactcc gggcggcgat gaaatgcgtc atgagcggaa agcagtgcgc tattctggtg
     7081 ccgaccacgg ttcttgccat gcagcactac cagacggcgc tgcggcggtt tgagcatttc
     7141 ccggtgaatg tggtgatgct gtcccggttc gtctcggcaa agcggcagaa ggagatcctc
     7201 aaggagatca aaagcggcaa ggcggatgtt atcatcggca cccaccgcat tgtgcagaaa
     7261 gacgtggaat tccacgccct
//
LOCUS       DAWENO010000105         7263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4180_length_7263_cov_14.000139, whole genome shotgun sequence.
ACCESSION   DAWENO010000105 DAWENO010000000
VERSION     DAWENO010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4180_length_7263_cov_14.000139"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1782 a         1958 c         2032 g         1491 t
ORIGIN      
        1 ttccacaacc tgtgcaaagt acgacaccat gcggcagggt tttccacgaa attctccaca
       61 aactttccct ttccactcca caatctttcc ccttttccac gcagaaaaaa cttctccact
      121 tttccggatt ctttccacga atgtcctgtg gggaaaaagt ccccggaatt tccggtaaaa
      181 agctgggttt ccacttttcc ggggtctcta ctagtactac tatatctttt ttatctttct
      241 attctttctt tgtgcagctt cgctgcctct tttctcgaat aaggcggtgt ctcccggaga
      301 cgtacaattt gaaattgttt ctccagagcg gacacatctg caaaaagcac agaaactgcg
      361 tcagatttcc tgtcttccca ggcggaacaa gacggcagcg gcattccgat cagaaagaat
      421 cgggcagaat caagttctgc ttttcagaac tgccggaaaa atcacgcggc agaaaaattg
      481 taattttcag ccgatgcggc aaggctgtgg aaagcccgtc ccgtgcggct tatcataaaa
      541 ggaagagatt catgttaaaa tttacttgtg acagacagac gttttacacc gccattacac
      601 acgtggcaaa gggcgtctct caaaaatcca cgatcccggc gctggagcgg ctgaagctcc
      661 gcctggatca cgacaatctg gaactgacgg gctatgacct ggaattcggc attcagcgga
      721 gtatcgaggt tgaatccgac tgctccggcg aatttgtcat tctgccccgg ctgctgagcg
      781 aggcggtacg ccgcttttcc ggcgacgtgg tcaccatgga ggtggacgac aactatgtgg
      841 taaagctgtt ctgcgatgcc acagagttcc agatctccgc catgtcctct gaggaatatc
      901 cggcgctgcc cagtctggat ctggcggcag gtatgcagat cgaacagccg gtgctgaact
      961 ccatgatccg gcagaccagc tacgccactt ctgtcagcga ggcaaaaccg gtgctgacag
     1021 gtgagctgtt cgaggtagag agcgacaccc tgcacgtggc agccattgac ggctatcgcc
     1081 tggcagtgcg ccaggaaccc atttccagtg cggaaaacta ccgctttgtg gtaccgaaac
     1141 ggaccctgct ggaggtggca aatatgctcc acgacgacgc cgaagaactc tgcaccattt
     1201 ccgcagaccg ccggcacgtg gtattcgaat tcaacggcta caccgtattt tcccggcttt
     1261 tggagggcga attccacaac taccggagca gcatcccgtc ctcttttgag acggagatcc
     1321 tgctggacac cagcgaactg acacgctgcc tggagcgctg ctctctgctc attagcggca
     1381 agtacaatgc gccggtgcgc tgcaccttct ccggcggcag cctgggcgta tggtgcaaga
     1441 cgggcatcgg cgagatcaac gacaagattc cggcagacat taccggtccg gatgtgacca
     1501 ttggatttga caaccgtttt ctgctggacg cagtgcgtgc ggcagactgt gacaaggtaa
     1561 agatccagct cagcggcgga aaccgtgtgg caaagctggt gccgccgcag ggcgaaagct
     1621 ttaccttcct gttaatgccg atccagctgc gcaactagcg gcaggttgcc gctgaccctt
     1681 cccccaattt agcgttcaag aaaggcagtg ttacggcggc ggcagccgcc tgttacccct
     1741 ccgggggatt tatgtttctg gtaagctgtg aaaacactgc cagtcgtggc atacactgcc
     1801 gttccgaaca agggtaggcg agatcttttc tttttttgct tctttttttc ttttcagcaa
     1861 agaaaaaaag aagagtaaac tcttcgtgta aactttgcgc aagaaagcaa aaaagaaaca
     1921 agcgcagcaa acgctgtaaa aaagcaaaaa ccatcctcaa aacaaacacc cagaatatca
     1981 acacagcaca tacaagaaaa ggcaaaaaaa gcgtttgcaa gcccaaggcg gacagcgaga
     2041 gaaacgagcg attccgccga acgggtttcg ggagtgagac ctaggataat atatccccct
     2101 gccctatcgg gcatctcctc taaaagagga gacaatttcg caaagcctcc tctacagagg
     2161 aggtggcacg cagtgcggtc ggagtcagag gagtcacggg cgaacaatgt tcgcccctac
     2221 ggtgtgcctt tttaagaaaa caaaccattt tttaagatag tcccccttga aaaggcgccc
     2281 gtagggtgtg ccttgtgctg ggatgtcagc gtaagctgac agggggatta aaccctcggt
     2341 caagcctacg ggcgtttttc caagggaggc aaaagactcc tctgcccatc catttcaaaa
     2401 tgtttcacgt gaaacatcaa agcttgcgcc gtgtcatttg tgtaaatttg cacgcacact
     2461 gccggcaaaa acacagatgc gccgggcttt ccgaaaccca aacgaaacca tccatgcgga
     2521 tgtctgtacg gcaaaacgaa aaaacgcctg cggacaccag ggagtgtcaa cacaccctaa
     2581 aaaacagcgg ctaatctgcc gccggaggat atcatggctg aaaaaaatga gacaataacc
     2641 gtgtcgatcc acacggaatt tatcaaactg gattccctgc tgaaattcgc agggctttgt
     2701 gacaccggcg gattcgccaa ggaactggtg cagcagggcg ctgtccgggt caacggcgaa
     2761 gtctgcacca tgcgtggcaa aaagatccgt cccggcgatg cggtgacggt ggataagtat
     2821 acggttcggg tgaccgctga ggcatgagac tgacggcact gtctgcggac ggtttcaaaa
     2881 acctggaatc ggtctcgctg atccccgacc cgtcctataa tctgatcctg ggcgaaaatg
     2941 cacagggaaa gaccaacctt ctggaggcaa tctgggcgct caccggctgc cgcagctttc
     3001 gtggcacacg ggaacgggac tatctcccgt tttccggcgg caacctccag gtagaggcga
     3061 aattccagga cgcacgccgg gaacagtccg tgcggctctg catggttccc ggcagcgttc
     3121 ccgaaaaaaa gttctggtgc aatgacattc cccagaagag cagcgccctc ttttccgtgt
     3181 tccactgcgt ggcgtttacg ccggatgacc tttccctggt ggacggcggc ccggagcggc
     3241 ggcgcacctt tctggatatg tgcagcgccc agctccatgc gccgatgctg ggactggtgc
     3301 ggcgctatca gctggcactc cagcagcgca acgccgtgtt gaaacagcac ctgcccgaat
     3361 cccaggcaga gggcttgctg ctggtctggg acaaacagct ggaacagctg ggaacggaga
     3421 tcgccgtcca gcgcagcatc tacgtgcggc ggctggcaga ggtctgcgtg ccgctgtatg
     3481 cccggatcgc acggggcaga gagaccatct cgctccacta tcagtccagc gtgttcggca
     3541 gagatgccca ggaaacactt ccggagcagg cgacacccga actggtgcgg cagtacggcg
     3601 aaaaaatgcg ccagagccgt gcctcggatc tccagctggg acacaccggc agcggtgcgc
     3661 accgggacga cctgttcctc gccatagacg gcaagccggc aaaggagttc ggctcacagg
     3721 gacagaaaaa aagcctggcg ctgtcactga aactggcgca ggcggagatc tacagcaagc
     3781 gccggaagga atcgccggta gtgctgctgg atgatgtcat gggcgaactg gacgaggctc
     3841 gccaggcggt ggtgtctacc atcgtccggg agatgcaggt gtttgtgacc acctgtcacc
     3901 cggagtccgt gctttcccca aaaacgggac ggcgttttct gttgaaagac ggcagactca
     3961 tggacgagcc gtcctaacag ccggaaactg cgtgaaattt agttttccac agactgttga
     4021 aaactgtgtg gaaactttgt ttgaagctgt gcaaaagcag ggatccggac ggtttctgcg
     4081 gaaatttgtc cgattccgtg tgcgcagtgc cggctgtttc acgtgaaaca atcccggaaa
     4141 tcacgaaaag caaaggaaac caaacagatc tttcccaagc ggaaaaattg aggtaataga
     4201 atatgacaga tgaaatgaaa caagacgcac agagttatga cgaaagtcag attcaggtgc
     4261 tggaaggact ggaggcagtc cgaaagcggc cgggtatgta catcggttct accggcccga
     4321 agggactgca tcatctggtc tatgagatcg tagataacgc cattgacgag gcgctggcag
     4381 gattctgcac cgagatcacc gtggacattc tcccggaaaa cgtgatccgt gtttcggata
     4441 acggcagagg catcccggtg ggcatccacc caaaagaggg catctcggcg gcaaccgtcg
     4501 tgtacaccat cctccacgcc ggcggtaagt tcggcggcgg cggctataag gtctccggcg
     4561 gtctgcacgg cgtaggtgca tccgttgtaa acgccctgtc ggaatggctg aaactcaccg
     4621 tcaaggacgg cagccacatc cacttccagg aattccaccg gggcgactac agcgaaccca
     4681 tccgcatcat cggcgacacc actgagaccg gtacgaccgt tgagttcaag gcagatccgc
     4741 tgatcttcga ggagaccacg gaatatgact atgaagtcct gctgaaacgg ctccgggaac
     4801 aggcgtttct gaatgccggc atccgcattg tcttttccga caagcgcagc gagaccgagc
     4861 cacgcactga gattctccac tatgagggcg gtatccgcca gttcgtggag tatttgcaga
     4921 acaagcgtgg gctggaggca ctgtccgagg atgtgatcta tgtttccggc atggacggcg
     4981 attctttcgc cgaagtcgct ttgcagtaca acgacagcta taacgatctg atcctgtcgt
     5041 ttgccaataa tatccatacc gcagacggcg gtacccacga gaccggtttc aagaacgctc
     5101 tgaccaaggt catcaacgaa tacggcaaga agttcggact gctcaaggac ggacagaaac
     5161 tgctgggcga cgatgtccgg gagggtctga cggcgatcgt ttccgtcaag ctgacggagt
     5221 gcgagttcga gggtcagacc aagggtcgcc tgggtaatcc ggcagtcaag ccctttgtgg
     5281 aaaagatggt ctttgacaag ctgatgagct acttcgagga gaaccccacc gtagcacgtg
     5341 ccattttcga caagagcatt tccgcacagg ctgcccgtga ggcggcgaaa aaggcgagag
     5401 acaccgcacg ccggaagtcc gccatggaaa gcgcatccct gccgggcaag ctggcagact
     5461 gccaggagaa atccaccgag ggtacagagc tgtacatcgt cgagggtgac tctgcgggcg
     5521 gttctgccaa ggaaggcaga gaccgccgct atcaggcgat cctgccgctc tggggcaaga
     5581 tgctgaacgt ggaaaaggca cgcatcgacc gggtatacgg caacgaaaag ctgatgccgg
     5641 tagtcaccgc tctgggaacg tccatcggcg aggacttcga catcaacaag ctgcgctacg
     5701 gcaaagtcat tatcatggcg gatgccgatg tggacggttc gcatatccgt accctgctgc
     5761 tgaccttctt cttccgcttt atgcggccgc ttatcaccaa cggcaatgtc tttctggcgc
     5821 agccgccgct gttcaaggtc tccaagggca agaagtataa gtacgccttc gatgaggcag
     5881 aacgggacaa gtatatcgca gaattcgccg acgcagacgg cagcaccgcc aaggtgggcg
     5941 tacagcggta taaaggtctg ggtgagatga gttccgaaca gctgtgggag accaccatga
     6001 acccggagac tcgttccatg atccgtgtgg acatggaaga tgcagaaaag gcagacgaga
     6061 tcttcaccat cctcatgggc gacaaggttg cgccccgtaa ggaatttatc gagaagaacg
     6121 cacgctatgt ccagaatctg gacgtgtaaa gaggaaacgc tatggaaaag acaacggcgc
     6181 tgccggtatt cgacaccact gccccgtaca atatcaaacg ggaatattac aagaccggat
     6241 tcgagtgctt tcagaagaag tttatcttaa agcgcaactg gatcatgctg gtgctgtttt
     6301 tcattttgtt tgtgtccttt gtggcatcgg ctgtggcaaa ccccagcaat aagacggcgt
     6361 actttctcat gatgatctgc ctggcaggaa tgttcgtgct gtggtataat ccacggaaac
     6421 aaaagcggat ggtgctggac gccgtccggg agctggagtt cgagcagttc accgcctgca
     6481 atgacggcaa ggtgctgcgc atccagatct gtcagaccga cgacgaagag gaacgcattc
     6541 cggaatcccg tgtggtcatg gcgactgcct atgtgcagga gttcgacgag ttctatctgg
     6601 tctgcgacga aaagaagatg ttctacatcc tgccaaagtc ggcgctggca tcccctgcgg
     6661 cagaactgcc cgaagaggcc agtgcggaaa gcggtgcaga cagcgaggca tgattgtttc
     6721 acgtgaaaca ttggcgggat ggtttttctg ttgagtggaa ggcgttgact cctctgccct
     6781 atcgggcatc tcctctaaag aggagacaat tccgcaaagc ctcctctccc agaggaggtg
     6841 gcaagccgta ggcttgacgg aggagtttcg ggcgaacaat gttcgcccct acaggtttcc
     6901 ctctttgaaa atgggaaaac aactcctctg ccctatcggg catctcctct atgagaggag
     6961 acgttttgca aagcctcctc ttttagagga ggtggcaagc cgctggcttg acggaggagt
     7021 ttcgggcgaa caatgttcgc ccctacaggt ttccctcttt gaaaatggga aatcgactcc
     7081 tctgccctat cgggcatctc ctctacagag gagacgatta tgcaaagcct cctcttctag
     7141 aggaggtggc aagccgtagg cttgacggag gagtcatgca ccccaccaaa actccccacc
     7201 cgtttcccat gaaacgaccc aaatcacaac aaccattcgc cggtgatgcc gccatgatag
     7261 gaa
//
LOCUS       DAWENO010000106         7070 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4308_length_7070_cov_11.066714, whole genome shotgun sequence.
ACCESSION   DAWENO010000106 DAWENO010000000
VERSION     DAWENO010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4308_length_7070_cov_11.066714"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1750..1759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3488..3497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1607 a         1776 c         2114 g         1553 t
ORIGIN      
        1 cttttcaagg gggacatgat gcgtggtaat aagcctccct tgaaaaggga ggtggcacga
       61 agtgccggag ggatttcagc acaaagtacg ttccgaaaga actcttttca cgcacaacca
      121 aatcaaacac aacaaggcag ccatgctccg gcgaacggtg tgtggctgct tttttgcata
      181 cccgaccggg aaagcgcata agctgtacca aaaacgggag gtgtgcctat gaaacgctgg
      241 cttgcggcaa tttgtgcggc ggtgcttttt gggatgctct ggcgtgtgcc ggcggtctgt
      301 gccgcagaag aaccggagat cccggcggca gcctatgcgc tgatggagac ggagaccggg
      361 acgcttttgc tggaatccca cagcagcgac cggatggcgt gcggcgcaat ggcaaagctg
      421 atgactgcct atctggcggc ggagcaggtg gagcagggga attggacggc ggacaccgtc
      481 ctcacggcga cagatgcggt caccggcaca aagggtgctg tgatctggct caccgccggg
      541 gaagagatga cggtgggcga cctgctgaaa gggctgatcg tggggaacgc caacgatgcc
      601 gctgttgtgc ttgccgtggc tgcgggcggc gatgcggaga cctttgtcat ggacatgaac
      661 gcccgtgcct ttgacctggg gatgcgtgac acccggttcg ccagtcccca gggcagcccg
      721 gacgacgggg cgtataccac ggcggcagat ctggcacggc tctgctgcgc tctggcgaaa
      781 caggactgtc tccgggaata tttccgcacc tggcgggatt ttctccgggg cgacgccacc
      841 gagctggtga acgagaacac ctttgcccgg acggatgaga ccagcatcgg ctttaaggcg
      901 tgccacagcg aaagcgaggg ctggagcatt gccgccgggg cagagcggaa cgggatgcag
      961 tgcgtggcgg tggcgctggg gtgcggcgac acggacagcc gctttgccct ggcgaaacag
     1021 ctgctgcgga aagggttcgc cgggtggaag gtggtacagc ccggattttc cggggaattt
     1081 ctctatcctg tccagatccg tggcggcgtg gaacgtgccg tgctggcaga accgggacgg
     1141 ctcaaggcac tggtcatccc acggagctgc ggcgagctgg aaaccgtgat ggtgctgccc
     1201 ggctatctcc gtgcgccggt gcggaaaggg cagaaactgg gcagtgcggc gttttaccag
     1261 ggcgataccc tgctctatga aaccgacgtg gtggcggcag atgccgtccg ggaacggaac
     1321 ttctgggatg cgctggaaca aataacggtc aaaatgctta aattgtagaa gataaatttg
     1381 tgtagattat acaagaatcc ggatacccct tgcaatctgc cgcagaatat agtatgatat
     1441 aagtggtaaa gtggttggac agcacaagtg tgccggctgg tctcaatggc tcccttgaac
     1501 ggggagctgg cagccgcagg ctgacggagg agtcgtttcc tttgaaaagc ggcatccttt
     1561 ccgggatcac cgcacacgct gcccggcgcc ttgagaagaa agtggaggga aattgtatta
     1621 tgtcttctgt aaagctgaat acaaaatatc tggcaaatta tgtgaatccg gacgaactga
     1681 ccggaatccg tcatcaggta gaggcatctg caaagatgct gcacgaaaag agcggtctcg
     1741 gctcggattn nnnnnnnnna ttctgaggta ctggttgtaa tcggtatcgg cggttcttat
     1801 ctgggcgctc gtgctgccat cgaattcctg aagtcgccgt tctacaacaa tctgaagaag
     1861 gacactccgg acatctacta tgtgggcaac aacatcaacc ccacctatct gaatgaagtg
     1921 atctccatct gcgagggcag agacttctct gtcaacgtca tttccaagtc cggcaccacc
     1981 acagagccgg cgctggcatt ccgtgtgttc aagcagctgg tggaaaagaa gtacggcaag
     2041 gacggcgcaa aggatcacat ttttgcaacc actgacaagg caaagggtac gctgaagtcc
     2101 ctgtccgata ctgagggcta tgagaccttc gttgtaccgg atgatgtggg tggacgctat
     2161 tctgttctga ccgctgttgg tctgctgccc atcgctgttg caggctgcga catcgaggca
     2221 ctgatggcag gcgccgcaga ggcacagaag gaactgaccg cagacttcga caacaacgac
     2281 tgctacaagt acgctgcact gcgcaacatt ctgtaccgca agggcaagaa tgtggagatg
     2341 ctggtatcct atgagccgag ctttgtgacg atggcagaat ggtacaagca gctgttcggc
     2401 gagagcgagg gcaaggacaa caagggcatt ttcccgtcct ctgcagtatt ctccaccgac
     2461 ctgcactcca tgggtcagta catccaggaa ggcagccgca ttctgttcga gactgtggtt
     2521 gacatcaaga agccgaagca ggacttctat ctggaaccgg acgcagagaa cctggacgat
     2581 ctgaacttcc tgaccggtca gaatatgtct gtcgtaaacc gcaaggcgct ggaaggcgtt
     2641 gtgctggcac acacagacgg cggcgtgccg aacatgatcc tcgaggtcga ggacgtgtcc
     2701 gaaaagagcc tgggctatct gatctacttc ttcgaaaagg cttgcgctgt ttccggctat
     2761 ctgctgggcg taaatccgtt caaccagccg ggcgttgaaa gctataagaa gaatatgttc
     2821 gcactgctgg gcaagccggg ctatgaggac cagaaggctg ctctggaggc aaagctggct
     2881 gaataagcgg caaaaaaatc aagtgttcaa cagccggatc acggctgtga cggaataaat
     2941 ctgtaccgca tttcggtgca aaaaaaggag ttttcatcat gattcgactg attacaggta
     3001 aaaaaggaag cggcaaaaca aagatcatta tcgatagaat taacgcagca gtaaaagaca
     3061 ccaacggctg cctggtttgc atcgaaaagg gcgagacact gcgccgttcg atcagctata
     3121 aggtaagatg gtgcgatacc gagcagttcg ctatcgacag ctttgatgcg ttctatggct
     3181 ttgttgcagg tatgctggcc ggcaactatg acattaagga gatctatgta gacggcatcc
     3241 tgaagatcgc cggcgctgat ttcgaggcac tgggccgcac actggaaaag ctggacaagc
     3301 tgaccggcga cgattccacc gtgaccttca ctgtttccgc agatgcagct gaactgccgg
     3361 catctgttac caagtttgtg gactaatttg tccccggaca agggaaaacg ctggtttttt
     3421 cggttttttt gaaaaaggac ttgacaagcc ggcgtgaaat ttgttataat aaatgatgca
     3481 atgcgtgnnn nnnnnnntga ttctacagct gcgggagatt tttcaaatag agggtatgca
     3541 ccttccggtg tcctatgaga tcacgccgga ggaattgtct gaggtgcgtg gctatacatt
     3601 tgctgcaccg gtggcggttt cgggcgagtt ttataaccgt gccgggatcg tgacactgaa
     3661 atataccgtc tcctgtacgc tggatgtggt ctgtgaccgt tgtctcacgg agctgaagcg
     3721 ggattattcg tatgactttt ctcacacggt cgtaccgtct ttgcagagcg aaggggatat
     3781 ctatgacaca tacctggtcg cacaacacga cagcattgat atgaatgaga cagcaatctc
     3841 agatcttttg ctgatgctgc cgacaaagat gctttgccgg gaggactgca aggggctgtg
     3901 cgatatctgt gggtgcaatc tgaacgagcg cacctgcaac tgtcgaaagt agaaggaggt
     3961 gccaagacat ggcagtaccg aagaataagg tctccaaggc aagacgtgac aagagacgtt
     4021 ctgctgtttg gaagctggac gcacctgcaa tgagcacgtg cagcaactgc ggcgcaatta
     4081 aggctccgca caaggtttgc aagaactgcg gatattataa gggcgttgct gttctgaaga
     4141 tggacgatgc agaataagtg ctgactgaca agacggtttc gttttgtacg aggaggagag
     4201 gcggctggtg tcgcctctct tttttgtatg tttgtcggtt tttggggtga tttattgtcc
     4261 cccttgaaaa ggcgcccgta gggtgtgcct tgtgctggga tgtcagcgta agctgacagg
     4321 gggatttatc cctccgtctg ctctttaacg gagcatccac ctcgtactga gcggtcccct
     4381 ttgtcaccta cggtgacatt tccccacccc gtggggaatc acccttttca agggaggcta
     4441 tttagaggtg taggtttgac tgaggaatag cgtatcaaaa tatgaaatgt tatgtgtttc
     4501 taccgtaggg gcgaacatcg ttcgcccggg ttgactcctc tgccctgccg ggcatctcct
     4561 ctaaagagga gacgatgcgt gtatataggc tccttctttt agaggagctg gcaagccgta
     4621 ggcttgactg aggagtaacg cactaacggg cgaacaatgt tcgcccggga cgactcctct
     4681 gccctgtcgg gcatctcctc taaagaggag acgaatgcgt gtatataggc tcctctttag
     4741 aggagctggc agccgtaagg ctgactgagg agtgacacac caacgggcga acagtgttcg
     4801 cccctacaga gcgactttac acatcgaccc gataacggaa accatacgcc ccctgttctg
     4861 cacacccatt tgcaataaaa atttacggcg acaccgcaca aaaaccccgc aggcgtgctt
     4921 tgtgcgatgt tgccttatga cgataaagga gatgaaatca ctatggcatt acctgttgta
     4981 gctgtggtgg gacgacccaa tgtgggaaag tctaccttgt ttaataagct gatcggtcag
     5041 cggctctcca ttgtggagga cacgcccggc gtgacccgtg accggatcta tgccaagggc
     5101 gagtggcgtg atcgttcgtt catgctggtg gacaccggcg gtattgaaac agtcacagac
     5161 gacgtgatcc tcaagcagat gcgccgccag gcggaacttg ccattgagcg tgccgatgtg
     5221 atcgtgctgg tgacagatgt gcgctgcggc gtgaccgcag acgacagcgc cgtggcaagt
     5281 atgctccaga agagcggcaa gcccgtggtg ctggcggtca acaagtgcga ctctatcggc
     5341 gagccgccga tggagctgta cgaattctat aacctgggca tcggcgaccc gtttcccatc
     5401 tctgcggcgc acggtcacgg caccggcgat atgctggatg cagtctatga cctgctgcct
     5461 ccggaggacg agctggagga cgatgaggat gccatccacg tggcgatcat cggcaagccg
     5521 aacgtaggca agtcctcgct ggtgaaccgc atcgccgggg aagagcgtgc catcgtgtcc
     5581 aacatcgccg gcacgacccg tgacgcaacg gataccgtgg tggaaaacga agagggaaag
     5641 ttcgtcctca tcgacaccgc cggcatccgg aaaaaagccc gtgtcaccga gcgtatcgag
     5701 cattacagcg tgctgcgtgc ctatatggcg gtagaccgtg cggacgtgtg cctgattctc
     5761 attgacgcta ccgaaggctt tacggaacag gattccaagg ttgccggata tgcccacgaa
     5821 cagggcaagg cgtccatcat cgtggtcaac aagtgggatc tgatcgaaaa ggacaacagc
     5881 accatgaagg agttcgagga aaagctccag cgggatttca gctttatgtc ctatgcgccg
     5941 tttcttttca tttcggcaaa gaccggtcag cgtgtgccga agctgttcgc caagattcac
     6001 gaggtgtacc agcagaacgc catgcgcatt tccaccggta tgctcaacga tgtgctggca
     6061 tatgccacca cccgtgtcca gccgccgtcg gataagggac gccgtctgaa gatctactat
     6121 atgacccagg caagcaccaa gccgccgacg ttcgtcattt tcgtgaaccg tgccgacctg
     6181 ttccacttct cctatcagcg gtatatcgag aaccagattc gctcgacctt cgggctgacg
     6241 ggaacgccgg tgcgctttgt ggtgcgggaa cgggcgagag acgaaaagta acaggagaaa
     6301 acgatcatgc tgatactcgc aattttgctt tcggcggtgc tgtcctacct gctgggcagt
     6361 ttcaacagct ccattttggt ggtgcgcctg ctgaaacaca aggatatccg ggactatggc
     6421 agccacaatg ccgggctgac caatacgctg cgctgttttg gaaaaggctg tgcggcgctg
     6481 accctggtgg gagacctggc aaagggcatc atcgccgtgc tgctctcccg tggcatctgt
     6541 gaacttttgc ataccgggct gacggcgcag aacgatgtgc atttcatcgg ctatatcgcc
     6601 ggcatcttcg ccattctggg acacgttttt ccgctctatt accacttcaa aggcggcaag
     6661 ggcgttctgg tgggcgtttc ggtctttctg ggaatcgact ggaaagtctt tctctgtctc
     6721 atcgtcattt ttgccgttgt gctggcgatc tcgaagtatg tttccctcgg ctctatcgtg
     6781 gcatcggtct gctgtcccat tgtgacgttt ctgttccagt tctggcagcg tggcgacctg
     6841 cccatgtggt atctttggct caacaccggg ctggcggcgc tcatgggtgc gtgggtcatc
     6901 tggatgcacc gcaccaatat ccagcggctc agagccggga acgagaataa gttttcgttt
     6961 cattcgaaga aggcgtaggg gttgactcct ctgccctgtc gggcatctcc tctaggagag
     7021 gagacatttt ggctcctctt ccagaggagc tggcaagccg taggcttgac
//
LOCUS       DAWENO010000107         7038 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4338_length_7038_cov_8.901475, whole genome shotgun sequence.
ACCESSION   DAWENO010000107 DAWENO010000000
VERSION     DAWENO010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4338_length_7038_cov_8.901475"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1222..1231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3594..3603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1834 a         1542 c         1892 g         1750 t
ORIGIN      
        1 tgtcccgccg caccaaaaac aatccttgtc tgatcggtga acctggtgtc ggcaaaaccg
       61 ccatcgccga gggaatcgct ttgcggattc attccggaaa tgttccgatg cacttgaaaa
      121 acaagcaggt atacctgctg gatctgacct cgctggttgc cggaacacaa ttccgtgggc
      181 agttcgaaag ccgtgtcaag ggtctgatcg cagaggtaaa gcaggaggga aacatcatcc
      241 tgtttattga cgaggttcac aatctggtcg gcgcaggaaa ttccgaaggc tctatgaatg
      301 cggcgaacat tctgaaaccg gcgctttccc gtggcgagat ccaggtgatc ggtgcaacaa
      361 catttgaaga ataccgcaag tatatcgaaa aggattctgc gctggaacgc cgtttccagc
      421 cggttactgt caacgagccg accattgacg agaccgttga ggtgctgaac ggcattgcaa
      481 agtactacga gtcctatcat cgggtacaca tttctccgga aatgattcgt ctctgcgctg
      541 ttctggcgga acgctatatc acagaccgtt tcctgccgga taaggcaatc gacctgctgg
      601 acgaaagctg tgcctgcacc aatctgcgct ctccggaaat cgaggcgtat gatacgctga
      661 tgcagaaaaa ggaagagctg gagcagaaag aaaaggcact ggaagacgaa acagagatca
      721 attatgagga actggcacag gtaaagggcg agcggattcg cctggatgcc agcattgaag
      781 aggctgccaa gaaactggac gatgtgcagg tgacaagtga tgatctgtcg aaggtgatcg
      841 aactgtggac gggaatcccg gcacacaaga ttgctgagac ggaattcaca aagctggcga
      901 atctggaaga tcgtctgaaa tcccatatca tcggacagga tgaggcagtc gacctgctgg
      961 caaaggcgat caagcgcacc agagtgcagc tgagtcctcg ccgccgtccg gcatcgttta
     1021 tctttgttgg tcccactggc gtaggtaaga cggaactggt aaaggtgctg gcagaagagc
     1081 tgttcgactc caacgagccg ctgattcgtc tggacatgac cgagtttatg gaaaagcact
     1141 ctgttgcacg gatgatcggt tctcctccgg gatatgtcgg ctatgacgag gctggacagc
     1201 tgacagaaaa agtccgccgc cnnnnnnnnn nttctgatgc agattctgga tgaaggcaag
     1261 atcgacgatg cgcagggaag aacggtaagc tttgaaaata cggttatctg catgacttcc
     1321 aatgcaggtt ctacggacaa gagtattggt gtcggcttta accgcacaga tactgagatc
     1381 tccaaggaaa aggcaatgaa gggcttgcgg gagttcctgc gtccggaatt catcagccgt
     1441 attgatgaga tcgttgtatt ccggaacctg gaaaagaagg atttcgaaaa gatcgcatca
     1501 ctgatgctgg atgaaatgaa gcagccgctg ttggaaaaga atatcacaat gcagtacgat
     1561 gatgcagcat tggcagccat tgcagaggat tcttacggaa agccgtatgg tgcacgtgac
     1621 attcgtcgtg caatccgcca gaccgtggaa gaccaggtgg catcgctgat tattgcccat
     1681 accggcgaga ttcggacgct gctggtttct gcaaaggacg gcaaggtaga cgtttcctat
     1741 cagtaagcga atctgaaatt gcaatataaa ccaaaaaaga ggagaacact tgatgttctc
     1801 ctctttttgc attagtatca tattatgttt attctggtgc agataaaaaa gaaaacagct
     1861 tttgcatttg caaaagctgt ttttggttgg ggtggaagga tttgaaccct cggaatgatg
     1921 gagtcagagt ccactgcctt accgcttggc tacaccccag tgtatttatg tcatctgcac
     1981 cagacgactt tgttattata ccacatttca agggtcttgt caacgaaaat ctacagaaag
     2041 atagaaagaa aaatatcact gatttaattc ggcaaaaaaa taagttttac aattgaaaac
     2101 tgcatgatat aaaaaggata aaagaaagaa aaaatagtca gaaatacaag accaaaatcc
     2161 gaatttcttt cactttctcg aaattgactt ttttcacata gaatgctata atagaaagca
     2221 cggctttgaa tgtagacaga aggaagaagg atgaatgatg agttcaaaga ccaaacaata
     2281 cgagatggat atgtgcagcg gtccgattct gcggaaaatg ctgatgtttg cactgccgct
     2341 gatgctctcc agtattttgc agctgctctt taatgcggca gacattgtgg ttgtgggcaa
     2401 gtttgccgga gacaattccc tggcggcagt cggctcaaat acagcgctga taaacctgct
     2461 gaccaacctg ttcattggac tgtcgatcgg cgcaaacgta gttgctgccc ggcactacgg
     2521 tgcaaaggcg tgggatgatc tgcgccggac ggtacatacc gccatgctcc tcagtatgct
     2581 gagcggagcg ctgctgctgg ttttgggcgt cattggcgca gagcaaatgc tgatctggat
     2641 gcagacgccg gaagaggtgc tgcctctggc aactgtctac ctgcgtatct attttcttgg
     2701 tatgatttcg accatggtct ataacttcgg tagcgccctg ctccgtgcag tgggtgacac
     2761 caaacgtccg ctgtatttcc tgctgtgtgc cggcgtcatc aatgtgattt tgaatctgct
     2821 gtttgtcatc ggctttcaga tggacgtcat gggcgttgca atcgccacag tcatatctga
     2881 aaccgtgtct gcactgttgg tactgcgatg tctcgtcaaa gaaaaaggcg gcatccatct
     2941 ggaactccgt gcaatgcgga tcgaccgaaa aaagatgctc cagatcctgc gcatcggtct
     3001 cccggcaggc tttcaaggcg ttgtctttgc gctgtccaac gtcgtgattc agtcatcggt
     3061 caacatcttc ggaaacattg tcgtagccgg aaattccgcc gctgccaatc tggaaggttt
     3121 cgtctatatg gcgatgaatg cgttttatca gaccacgctt tccttcgtca gccagaacta
     3181 tggcgccggg aaacaaaagc gaatcaaccg catcgtttta ctgggtgagg cgtgcgttat
     3241 tgtgacaggc actttattgg gaaatatggt tgtctttttc ggaaacgacc tgctccagat
     3301 ttactccaac aatccggaag tgattgccgc cggaatggtg cgcctgcact atatttccat
     3361 gatctatgcc ctatgcggca tcatggacgt gatggtaggc gcactgcgtg gcatcggtta
     3421 ttccattatg ccgatgattg tttctattgt gggcgtgtgt gtattgcggc tgatctggct
     3481 ggcgacggtg ttccagattc cggagttcca caaaatcgaa acggtttatc tgtcctatcc
     3541 ggtcacctgg atcctgacca gtctggttta cattgtcttt tttgtgtgga tttnnnnnnn
     3601 nnngaacatt attccccatg aggccgtaca cgagaccgga aagttcctga tcgaaattat
     3661 gccgctgatg tttattccgg cggcagtggg gctgctggag acctgggatg tgattgcgcc
     3721 ggcttggctg gaatatgttg cagtcacagt gatttccact tgggttgtca tgattgtttc
     3781 cggcagagtc acccagtttg tgattcggcg aaagagaaag gacgtgcagg aaaactatgt
     3841 ttgaattttt agagaattcc gccttttttg gcgttgcaat cagtgtagtt tcttacggca
     3901 tcgggatgct gctgaagaaa aagtttaagc tggcaatttt gaatccgctt ttgatttcca
     3961 ttgtgattac cattgcattt ctggcgctga ccggaatcag ctacgagaaa tatgacgagg
     4021 gcgcaaagta cctcaactat ctgctgacgc ctgcgaccgt ttgcctggcg attccgctgt
     4081 atgagcagct ggaactcctc aagcataact ggcgtgccgt gctgctgggc atcttgtccg
     4141 gcgtgctttc cagcctgggc gtgattttgg gaatggctct gatgttcggg ttcagccatg
     4201 aggaatacgt cactttcctg ccgaagtcga ttaccacagc aatcggcatg ggcgtttcag
     4261 aaaagctggg cggctacgtt tccatcagcg ttgccgccat catcattacc ggtgttctgg
     4321 gcaatatctt tgcagagatc gtctttaagc tgttccggat ccatgagccg atcgcaaagg
     4381 gaattgccct gggcacatct tctcacgcca tcggtacagc aaaggcaatg gaactgggcg
     4441 aagtggaggg cgcaatgagc agcctttcta tcgtagtagc tggtttgatt actgttatcg
     4501 gcgcaacgat tttttcccat attatgtgag gtcatttatg cagagtgaaa ccagagaata
     4561 tcaaaaggtg attgactatg tatcccaaat gattgccgat gggcagctga aaatcggtga
     4621 ccggctcccc acggaacggc aaattgcaga aacactgtcc attggcagaa actccacacg
     4681 agaggcgctg cggatgctgg aacacatcgg catgatttcc tgtaagcgag ggtccggcaa
     4741 ttatctgaca gacaatgtat cccggtctgt ttctgaaatg gtacatatga tgcttttgct
     4801 ggggcagacc gatcaggcgg agatctgttc gttccgtcga aatatggaaa aggcagtctg
     4861 ccgtgcgatt ctggatggaa aaactttttc ccgctgggag acccaggtgg ctgaaattct
     4921 gcaccaggcc tgcgaaacac aggaacggga aatgcagatc gagctggaca gaagatttca
     4981 ttatctgttg atccaggcga cagaaaaccg gttttggatt gctctgctgg agccgatcgc
     5041 agacgtctac cgcaagtgga tcgatgcggc tttgcagtcg gcaaacgact ttgtaaaagc
     5101 acagctgcag gcagagcatc aggcactgtt tcatgcgttg caaaacaatg attacgctgc
     5161 ctgtgaggct gcaattgacc ggcattatga ctatgtagac gcagagctat ctaagtcttc
     5221 gaaataattg aatcagaatg cgttccggaa agggaaacgc tgctgcactg caccaacgaa
     5281 tgtcacagtt tgtgcagtgc ttttttatgc aatatcccga ttttgataca ggaatccagt
     5341 ggaatctcca tgaaaaatct tgacagtctc aaaatatatg gtataataga aaatgcagag
     5401 caattcggga tgaaccgatt ttgcagcaag agttaatttg gaggtataaa ggtatgaatt
     5461 tttggaaaaa gctcactgcc tgtgcactgg cagctgttat gtcggctggc tttgcaggct
     5521 gcggcaattc cggcggcgat tcttccgaga aagaggagtc tgacgaagag gcggcaatgc
     5581 cctatgacag cgatttcaca atcggcgtag ataaggttgc agaggcaatg ggtgccggct
     5641 ggaacgtagg caatcagctg gaggcaaact ccggcggtaa ggtggatgaa acagtctggg
     5701 gcaatccgga gattacccag gaactgatct ctgctgtggc agacgcagga ttcacaaccg
     5761 ttcgtgtgcc ggtttcttat ctggaccgca ttgatgatgc gaacaactac accgtagatt
     5821 cagcgtggct ggatcgtgtg gaagaggtag tccagtactg ttacaatgaa ggcttgtatg
     5881 tcatcatcaa tatacacggc gatggatata acaccgttga ggacggctgg tttctggtaa
     5941 acggcgaaga ccaggatatg atttgcgaaa agtacgaggc agtttggaaa cagattgcag
     6001 agcgtttcgc agactatgat gaacatttga ttttcgagtc catgaacgaa gagtgtgacg
     6061 gaacatacaa cggcgacccg aaccgggagt attatgcaaa tctgaacaag tacaatcaga
     6121 tttttgtgga taccgttcgt gacaccggca aaaagaacac ccaccgctgg ctgctggttc
     6181 ccggttggaa taccaatgta gaatatacgg ttggggatta tggctttgaa atgccgacag
     6241 atgaaaaatg ctctgccgat gagagccgca tgatggtttc cgtgcattat tatgatccgt
     6301 gggattactg cggcacagaa gatctgaaaa ccatcctgtg gggcgaatac ggtgacaatc
     6361 tgattgaggt caatggtttc ccgaagatga acaaggcaaa atggggcgat gagtcctacc
     6421 tggatgacct gtttaccaag atgcaggaca agtttgtgaa gaatgatatt ccggttataa
     6481 tcggtgagta tggctgtatt gacaagtcct ctgcctatgc cgatttctcc ggtcagatcc
     6541 agggcaaccg tgcatactgg gatggatatg ttgcaggtaa ggcagcgtcc atgggtatga
     6601 ttccggtata ctgggataac ggtttcaatg gcgtatatgg attcggtttg ttcgatcgta
     6661 acacctatga gcagacacag ccggagatca tttccacaat cattgaggca gtcaagaata
     6721 aggatccgaa agctggtctg gacacagttg tagaaaccaa gacagaaaag accacagagg
     6781 cacacgctta catcggcatt cagacagaga tctatacttt ccgtaactcc tgcagcgatg
     6841 gaaagtacgg aaaggatacc gactacttca ataccttgat taagtggggt gacgatgacc
     6901 agatcattga caccggtgca aagttcacgg atgcaacgat cactgaggac ggcacttata
     6961 ctgtttctgt agatggctat gacttctctt cggacagtag caagctgaat atgctgtttg
     7021 tcagcaccga tttcgcat
//
LOCUS       DAWENO010000108         6932 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4409_length_6932_cov_7.622510, whole genome shotgun sequence.
ACCESSION   DAWENO010000108 DAWENO010000000
VERSION     DAWENO010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4409_length_6932_cov_7.622510"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1814 a         1665 c         1852 g         1601 t
ORIGIN      
        1 aaatcgacac ctctgacatt ttcgatgcca tggtctatgt tgcctatcgt ggcgttggat
       61 tagacggggg actcacggac gagcaggtcg ctgctgctga tatcgacggc gacggaaagg
      121 tggattccac ggatatctac tatatgctct attatgttgc gctccagggc gcaggcaaaa
      181 acccgtcctg gcaggatgtc atttattgat gaactttcga aaacagaacc cgaaagccgt
      241 tgtgtatgac gcacagcggc ttttttgcgc catttatcat accgcaaaaa aatatttgcc
      301 ggctgttcga aaaataaact tgacaagtta gctaaattac agtataatta gctaaaaagt
      361 aaccttacaa aagaaaggac gaagaaacgt ggcaaaagca gtacggatgt cagacattgc
      421 cgagcggctc catgtcagca ttgtaacagt ttccaaggcg cttgccggaa aggacggcgt
      481 gagcgaagaa ctgcggcagg agatcttgca gctagcagaa gaaatgggct atcagagcaa
      541 aaagttcaag ccggcagcaa caggaaagac gtataccctg ggcattgtga cgtcataccg
      601 atatatcgaa aagggacagt cgttctattg gaacttgtat gagcgcattc tgttttatct
      661 ggcgcagacc ggacatattg gcatcctgga ggtcgtgaca gacagcgccg agcaggagcg
      721 gcagattcca cggctggtgc aggaagaacg ggtggacggc gttattgtca tgggcaattt
      781 ctcgaagtcg tatcgggaaa tgctggcaga gaccgggatt cctctggtgg tgctggatgc
      841 ctttgatgca cggtatcacc gggacagcgt gatctccgac ggctattatg ggatgtatac
      901 catggtggac tatctcctgc aaatggggca tcggaatatt atgttcgtag gttcggtgaa
      961 cgccaccagc agtatcacag accgctatta cggctattgc cgtgccatgc gtgaggcagg
     1021 cgtcagcgtg acggaagact acgtcctgcc agaccgtgac gccgaaggaa aggtcaccat
     1081 ttctctggag catctgaaaa aaatgccctc tgcgtttgcc tgcaactgcg atatcacagc
     1141 atatacattg ctgaatgcac tgaaagaaca aggcgttcgt gtgtcagaag acgtctccgt
     1201 ggtgggattt gatgactata tcctggcagg gctggctgtg ccgccgctga cgacttatgc
     1261 cgttgacgta gaccacatgg caaaagtcag cgcacagcag ctgatccgcc gcatccagaa
     1321 accggatgca gatgtacgcc gcattgtggt cagcggcaaa ctggtgatcc gggagagcgt
     1381 gaaagatctg cgctagaatt tttccaaaaa acagaacatt cttgcggctg catggacgga
     1441 aatgtctgtg cagcttattt tttagctaaa aatttagctg taccacgaga tattgcagta
     1501 ttacacaaca aaaatctgat attgtttgtt taatatagct gatctgaata gaattctttt
     1561 gtgtaaaacg cagaaaagca aatttaagct taaatttagc ttgactttta ggtaaaaata
     1621 gagtaagata gaatcataga aaaacggaat acaaaagaga ttccgaaaac ctgtacgtga
     1681 aaaaaggaat aagattgcga tatctgaatg gaggaaatca gaatgaaaca acgagtaatg
     1741 gcattggtac tggcaggaat ccttgcagca gcaacaaccg cctgcggcag cacaggaagt
     1801 acatccggca aaaacaacga cgcaaagcca acagtagcct ataaagatat gaaggtgggc
     1861 gaaactgaaa ccgatttgaa agctgatctg aagattctga cgaaccgcac cgatctggtg
     1921 gattccgact tccaggacta catcagcgag ttcaacgcca tgtatccgaa catcaagatc
     1981 acttatgagg gcatcaccga ttatgacacc gatgccacca cgagactttc ttccggagac
     2041 tggggcgaca tctgctgcat cccgtctacc gtggacaaga gcgaactcag caactatttc
     2101 gaaaagatcg gcgattacgg tgacttcaag gatacctacg aattcgtaga taagcagatg
     2161 ttcaacaatg aggtttacgg catcccgaca atgggcaatg tcagcggtat tgtttacaac
     2221 aaggcggttt tcgaaaaggc aggcatcacc gaactgccgt ccgatccgga aaccttcctg
     2281 gcggatctga aactgatcca ggataagact gatgcgattc cgctgtacac caactacgct
     2341 gccggatgga cactgactgc atgggacggc tatatcagcg gcggtgctac cggcgatccg
     2401 gactttaaga acaacaagct ggtgcattcg aagaatccgt ttgcaaagca cgatgatatg
     2461 accggaccgt atgctgtcta tgatacctta tatgaggcag taaagcaggg cttgaccgaa
     2521 gaggatccga ccaccacaga ctgggaaggc tgcaaggcac agatcaacga gggcaacatc
     2581 ggttgtatgg ttctgggcag ctgggcagtg ccgcagatgc aggcaggcgg tccgaatgcg
     2641 gatgacattg catatatgcc gttccccatc agcgtggacg gaaagcagta tgcgtccgca
     2701 agcgctgact attgctacgg cgtcaatgta aacagcacca gtgaagagaa aaccgctgca
     2761 atgctgtaca tcaagtggct cacagaatcc tccggatttg caaaaacgca gggcggtatc
     2821 cccatcgtca agggtgatac ttatccggat tctctggaga gcttccagga tatcacactg
     2881 attaccgata atccggcacc ggcaggggaa gaagatctgt tcaataagat caacaacgat
     2941 tctgagatct ccctggattc cgattccaca cacgtggcag caatcgtaga ggcagccatc
     3001 ggcggcggaa agaccatgga agagatcgca gacgaatgga atgctgaatg gacttccgca
     3061 caggaaaaga gcggtgtaac accggagtaa atccatcgac gacgcagaaa ggaagtgcat
     3121 ggacatggca gagcggcata cagaagtcgg attgtccaag cggaaacggc ggctgcaatg
     3181 gtcgaatgtg aatgtacaga aagtggttgt cacgctgaca ttcctgtttg ttccgttgtt
     3241 cctgctgata ttgtttacat acctgccgtt tttcaaaatg gtgcagttca gcttttatga
     3301 tatgaagtat atcggcgagc gaaaatttgt ggggctggaa aattatattg atgttttcac
     3361 ccgggacgat tgtttcaatg cgctgaaact cagcttgtat tatatggtcg gttctgtggt
     3421 acagcttgcg ctggcactgc tgtttgcaac gatcctcagc tttaacccca aaggcggaag
     3481 tttcttccgt ggcgcactgt atttcccgag cctggtctgc ggcattgccg tcgggtttat
     3541 cttcaagtat ttctacaccc acggttttgt cctggactcc atcctcggct tgctgggaat
     3601 cccggaagat atgctgccgt tctggctcaa ggacacccgc atcaataact gggtgctggt
     3661 ggcatcctcc atctggcggt acatgggaca gaacatggta ttgtttatcg gcgcaatcgc
     3721 cagtgtagac aagacgcagt atgaggcggc tgccattgac ggcgcaaacg catggcatcg
     3781 tttccggtat atcatcctgc cgtccatccg cacgatcgtt gtgctaaaca tgattcttgc
     3841 aatttccggt tctctctccg tatttgaaat tccatatgtt atcacagacg gcggctttgg
     3901 cacatccaca tattttgttc tcatggataa gctggcacac gccaaccaga aggttggtct
     3961 ggcgtcggca atggcagtgg tactgctggt gctgatcctg gtagtcagcg gcattcaaaa
     4021 gctggcggaa aagaagatga actccggcgt tcgtgtcgga aagaggaggg cgagaaaatg
     4081 aaaagcaaaa gcgtttttct gaaaagactg ccaaagcaga tccttcagta tgccatcctt
     4141 ctgttcggcg cattctgtgc gctggttccc attgtcagct gcgtgactac cgcattcaag
     4201 accgatgaag agtaccgcac gaccagcgtc atgacactgc cggaaagctg gctgaacttt
     4261 gaaaacttca aggttgcctg ggtaaaggca gacatgggca gagcatttct caacagcttt
     4321 ctcattctgg tatgcgtgct ggtaggcagc accatgatct ccgcaatgct ggcatatgtg
     4381 ctgaaccgtt ttcagttccg tggcaaccat ctgatccgca atctgttcat ggtcgcagcg
     4441 ctgattcccg gcattgcaac acaggtcaca gtctatcaga tcatgaccgt gctgcatctg
     4501 gtcaacaccc tttacggcta tattttcctg atgctcggca cagatgtcat tgcaatttac
     4561 atctttctgc aatttttcga gaatctcccg aaaactctgg atgaggcggc gattctggac
     4621 ggatgcagct atttcggtgt attcttccgc atcctgctgc ctctgctgaa accggcgatc
     4681 gtcaccagtg cgattctgaa aggtgtcagc acctataatg aatattacat ggcaaatctt
     4741 tacttacagg ataagagcct gtaccaggtg gtatcgactt cgctctatac ctttacagga
     4801 ccgatgggga gccagtatca gtatatctgt gccggcgtaa tcctcaccat gatcccgatt
     4861 ctgattattt tcctgttctg ccagaaacaa atctacagcg gcatggcaat gggcgctgtc
     4921 aaggaatgat aaggaggcaa ttcacaatga aaccgatttt gacaaagaca ggcagcctgc
     4981 ccaatctgcc gtggcaggag cgcccggcag actgtactgc accggtatgg cgctaccagg
     5041 aaaatcccat tatctcccgg aatcccatcc ctggtgtggc acgcattttc aacagcgcag
     5101 tcattccgta tgaagatgct tttgtcggcg tgttccgggg agagcaggtg aacggcattc
     5161 cgtttattta cttcggcaga agtgcagacg gcattcactg gacattcgac caggaaaaga
     5221 ttcagttcct ggatgaaaac ggagaaccgt tccagccggt ttatgcctac gacccacggc
     5281 tggtggaagt ggacggcact tattatgtga tctggtgcca ggatgcctac ggcgcagcca
     5341 tcggcatggc aaagactacc gactttcaga catttgtccg ggtggaaaat ccgttcctgc
     5401 cgtttaaccg gaacgcagtg ctgtttccac ggaagatcga cggcaagtac atgatgcttt
     5461 cccgtcccag cgacagcgga cacacgccgt tcggcgacat tttcctcagc gagagcaagg
     5521 acatggagta ctggggacac caccgccatg tgatgggaaa gagcagccgc tggtgggaaa
     5581 acaccaagat cggcggcggc gcagcaccca ttgagaccag tgagggctgg ctgctgttct
     5641 accatggggt gacgaatacc tgcaacggat ttgtttacag catcggcggt gcaattctgg
     5701 atctggagaa tccgtccaag gtgaaatacc gctgtgaaaa cttcctgctg acgccggaac
     5761 agtggtatga agagcgtggc tttgtgccga atgtatgctt tccctgtgcg acactccagg
     5821 acggtgacac cggcaggatc gccatttact atggctgtgc agatagttat gtaggattgg
     5881 catttaccga tgccgagacc gtgatcgaat acattcagtc ccacgatgcg gcgaatgaag
     5941 atgaccgcag cgagggtaga aaataaggca acaccgcaca aagcccgcct gacggctttg
     6001 cacgcaatct gcttgctgat tttccgtcat attgcccaga aattccttgc cagacacaaa
     6061 gtatgcctgc gaaatttctg cgcaatctgc caaaaaatcc ggctgcgcat cttacgcaca
     6121 agctttgtgc ggtgttgcct taagacttca attaggagct gagcgcatat gaaacgactg
     6181 acaccagaag tgcagaccat gctggaacag aagatcatcc cgttctggct ttcgctgaaa
     6241 gatgagcgat acggcggatt tatcagctat gtagacctgg atctgcggcg gtatgccaaa
     6301 gcagaaaagg gctgtattct caatagccgc atcctctggt ttttctcaga ggctgccatg
     6361 ctgctccaca acccggaact gagccatgcg gcagatcatg cgtaccgctt tctgatgcgc
     6421 tactgcgtgg accgcaaggc aggcggtgtg ttctggtcgg tgacatatga cggaaagccg
     6481 ctggacacca ccaagcacac ctataaccag gcgtttgcca tttatgcgct ctccgcttac
     6541 tatcggctga ccggaaatcc cgaggcgctg caaaccgcaa ggcagctgtt tcagatcgta
     6601 gagatgcact gccgggatgc agaaggctac ggcgagtcct acgaccggtt ctggaatccg
     6661 cagtccaatg aaaagctttc tgaaaacggc gtcatggcag agcgtaccat gaatacgctg
     6721 ctccacgtgt ttgagggcta cagcggtttg taccaggcgg cgaagtcgcc ggaggtggaa
     6781 aaggaactgc gggcgctgct ggatatctgg tcggaaaaaa tgttcgatcc ggaaaaacgg
     6841 cggcaggagg tattcttcga tcggtcgtat cacagtctga tcgacctgca aagctatgga
     6901 cacgacatcg aaagcgcatg gctgctggac tg
//
LOCUS       DAWENO010000109         6879 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4449_length_6879_cov_10.019637, whole genome shotgun sequence.
ACCESSION   DAWENO010000109 DAWENO010000000
VERSION     DAWENO010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_6879_cov_10.019637"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4394..4403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5607..5616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1728 a         1900 c         1866 g         1365 t
ORIGIN      
        1 ctgcaaggca ctgcaggtgg accacagcga atacctgggc attctccgca agctgcgaaa
       61 gctgccccgg gtcaagcgtg tctttatccg ctccggcatc cggttcgact atgtgatggc
      121 ggacaaggat gacaccttct tcaaggaact ggtggcggaa cacgtgagcg gtcagctgaa
      181 ggtcgcaccg gaacacgtca gcaacgtggt gctggacaaa atgggaaagc cccacatcga
      241 gtgctatgag gcgtttcaga aacggttcta cgagatcacc aagagcatcg gcaaaaagca
      301 gtatctcgtc ccctatctca tgtcctccca ccccggcagc accctccagg aggcagtaaa
      361 gctgtccctg tttctccggg acaacggcat ccacccggaa caggtgcagg acttctaccc
      421 cacgccggga acgatttcca cttgtatgta ctacacggag ctggacccct acaccatgga
      481 gccggtctat gtcccgaaaa cacccaaaga aaaagccatg cagcgtgcgc tgctccaata
      541 ttatctgccc cagaaccgcc agctggtgct gtctgcactg aaacaggcag gacggcagga
      601 tctgatcggc acaaagccgg gctgtctggt cgcaccggaa cagcacgccg gcaattcccc
      661 aaaaccgtcc ggaaacggac agcgacaccc caaaggaggc gcatccccac gaaaccgcaa
      721 accatcttcc aaaaaatcac ggcggcactg atcgccgcag cacttccgct gctgagcgga
      781 tgcagtctgg acgatgccga aagctatctc accgacacca ttcagcaggc gttcggcggt
      841 gaagacggcg cagaggcaga gaacaacggc ggctccggca caggcagctg taccgtccat
      901 ttcctggatg tgggacaggg cgacagcgaa ctcattgtca ccgacaccgc cgccgtcctc
      961 atcgacgccg gcgaacagag cgccgcagac gacatcctcg ccgacctgca gcaatacggc
     1021 atcaccaagc tggactggat catcggcaca catccccacg ccgaccacat cggtggcttc
     1081 cccgatattt tgtcctatgc ggcgcagaac agcgatctga ccatcagcaa tgtgatgatt
     1141 ccccagctgc cggacgatat gatccccacc acacgtactt atgaaaagtt cctggacggc
     1201 gtagacgaaa acggactgat cctgacccag gcgacggata tcaccattga cctgggcagc
     1261 gcatccctgg agatcatccc ctcgccgggc aatgactaca gctctctcaa cgactattct
     1321 atctgcgcct atctcacctg cggcgacacc agctttttct tcaccggcga cgcatcgaaa
     1381 ccggaagaaa aagatctgct gcgttccggt gcgctggacg gcatccaggc agatgtgctg
     1441 aaggtgggac accacggcag ccgggaatct tccaccgccg attttctggc acagctccag
     1501 cccagctatg cagtgatctc ctgcggcgca gggaattcct acgggcatcc ccacagcgag
     1561 gcagtggagc gtttgaagca gtactgcggc gaccagatct ggcgcaccga cgaaagcggc
     1621 actgtcacca tacagtccga cggcgcatcg ctgaccgttt ctaccgaaaa gtgagggata
     1681 cttatatgat aatcttagat cgtctggaag agacgctggc tgtgctggaa acagatgacg
     1741 gcatggtcaa tgtgccacgg gaactgctcc cggcagatgt ccgggaaggc gatgtgctgc
     1801 ggcaggcggc agacggcact tacaccaccg ataccgccgc cacccaggct cgccgggaaa
     1861 aactcctggc acggacacgg cggatgactg gaaagtgagc gttggcgaag gatagtactg
     1921 agcgacctcc ttttcaagag aggaaaatta tttctcacca tgtccccctt gaaaaggggg
     1981 atgtcagcgt aaggtgacag ggggattttc cctttccaaa aacaactctg acgcattccc
     2041 acacgaattt ccgtttccgt cttgcatttt ctcagacaat gtgctatact atccataaac
     2101 gaaattattt ttcattatca ttcaaagcaa agtgaggtaa tatggaaaca gaccatttat
     2161 gtatcggcgg cggtccggca ggtgtctttg cggcactgac cgccgccgca aacggcaaac
     2221 gctgcatgat cctggaacac aacaaacagc tgggacgcaa gctgcgcatc accggaaagg
     2281 gacggtgcaa tctcaccaac aactgtgacc gggacaccct gctggcaaac attccggcaa
     2341 acggaaagtt cctgtacagt gcgctctccc ggtgtacgcc gcaggatgtg atggcttatt
     2401 tcgagggctt gggcgtgccg ctgaaaaccg agcgtggaaa ccgggtattt ccggtgagcg
     2461 accgggcgga ggatattgtc caggcgctgc aaaaggcagt ccagcgggca ggcatcccgg
     2521 tggtacacgg cgatgcggcg catctgatcc tggaacaggg cagatgcctg ggtgtccgca
     2581 ccgcagacgg cagagagttc cgggctggga caacgctcct cgccaccggc ggactttcct
     2641 accccaagac cggctcagac ggcacaggct atcggctggc agaagaggca ggtcacacca
     2701 tcgtgccgcc tatgccctcg ctgatcccgt tggtcaccaa agagaactgg tgccgggatg
     2761 ccatggggct ttccctgcgg aacgtgaccc tgcggctgta ccggggcgaa aaatgcgtct
     2821 ttgaagatct gggcgaaatg ctgttcaccc acttcggcgt atccggtcct ctggtactca
     2881 gcgcaagcgc tctcatgcga aagggcagcc cggaggacta ccggctggag atcgacctga
     2941 aacccggtct gacgccggaa caactggaca agcgcatcca gcgtgacctg gcagagagtc
     3001 ccaaccggga tctggggaac attctccacg ccctgctgcc ggcaaagctg atcctgcccg
     3061 tactgcggct gtgcggcatt gcaccggaca ccaaagccaa cacgctcacc aaggcgcagc
     3121 ggctcaccct gggcggctgc atcaaggcag tctcgctcca cgtccgggcg tttcgtccca
     3181 tcgaagaggc gatcatcacc cgtggcggcg tctccgtcaa ggaggtcgat gccagaacga
     3241 tggcatccaa gcgctgtgaa gggctgtatt tcgccggaga gatcctggat ctggacggct
     3301 ataccggcgg ctttaatttg cagatcgcct tttccacggc gtacagcgcc ggcattgcca
     3361 tggcagataa tttttagttc aaatatctga agattttgta tacataaagg agtttgtaag
     3421 tacttatgaa aacgatcaac atcgccattg acggtcctgc gggagcggga aagagcagca
     3481 tcgccagaga agtggcatcc cagttgggct ttgtctatgt ggacaccggc gcactgtatc
     3541 gtgccatcgc cctgttttcc acagaatatc agaccagaaa ggactttctg gacaatctgg
     3601 aggaacactg ccacctggaa ctgaaatatt tcggcggcac acagtgcatc ttcctcaacg
     3661 atgagaacgt gactgaaaag atacgcaccc cggagatctc catgaaagcg tcggaagtct
     3721 ctgccctgcc ggaagtccgg gagttcctgc tggagaccca gaaggacatc gcacgggaat
     3781 tcaatgtcat catggacgga cgggacatcg gcacggtggt gctgccggac gcagatctga
     3841 agatctatct gaccgcatcc gcagaagagc gtgccagccg ccgctatctg gagatggagg
     3901 acaagccgga caagcccacc tatgaccaga tcctggagga catcaagcag cgtgactaca
     3961 acgatatgca ccgtgcagtt tcgccgctcc aacaggcaga agatgccgtc ctcatcgaca
     4021 gcactaccat gggctttcac gaggtctgtg aggagatcat ggatctgatc gaacagcgcc
     4081 tggatgtgga gtgaagaagg aggtcatttt ttgccgtatc ccattgtctt tttcttttac
     4141 cgcatcgcct attatctggt gcggatcgca tttttcttct actaccgcat ctcctttgag
     4201 ggtcgggaaa atatccccaa gggaacagcc gtgatctttg cctccaacca ccggagctat
     4261 ctggatccgg tgctggtgtc aatggctgcg ccccatccgt tcaactttat cgccaaggaa
     4321 ccgctcttcc ggaatcccat tttcggaaac ttcatccgat tcatgggcgc attccccacg
     4381 gcgaacagca aggnnnnnnn nnnatgatat gctgaccgag gcgacaaagc ggctggaaaa
     4441 gcgccgccac ctcaccattt tcccggaagg cacacgccac acggacggca aggtgggcag
     4501 aggaaaaagc ggcgtctgtg tgctggcggc acgcagcggc aagccggtag tccccatcgg
     4561 gctgatcttc gactccaaca atctacactt ccgcagccgc atctgcgttc gggtaggaaa
     4621 gcccatctat gccgcagact acgggctgaa cgcccagtcc acgccccacg agatgcacgc
     4681 catgcgtaag gacatcatgg acagcatcaa atccatggtg gaagaaaatc cgccgttccc
     4741 gatcctgcac gatgtgccga aacaccgcac taccttcgag atcgcaaagg atcagaaacg
     4801 tgccgcacta gaacagaaaa aacaggcaga acagtctgcc gggacaacgg aggaataact
     4861 ggtcatggct gaaattaccg ttgctgagtc cgccggcttt tgctttggcg tagaccgtgc
     4921 cgtcaagctc tgctaccagg cactggagga gcatcacaac atcgccaccc tgggtcccat
     4981 catccacaac caggacgtgg tggacgacct cacccggcgt ggcgcacgga tcgtggactc
     5041 catcgccgag ctgcacccgg acgaatgcgt ggtgatacgc tctcacggcg tcagcgcaaa
     5101 ggtgtatgaa gaactggaac aggctgggaa tccctatgtg gatgccacct gtccgtttgt
     5161 ggcaaagatc caccgaattg tggaaaaaca taccaaagca ggagatttca tcctcatcgc
     5221 cggagatgcc aatcatccgg aagttgccgc aattgtcggg cattgtaaag ggaattgctt
     5281 tgtttttgaa agtgaaaaag ccttaaacga tttttttcaa aaaaatttag tggattctcg
     5341 caaaagactt gcaattgttg cgcaaacaac gtataatata ttagtatggg aaaagtgttt
     5401 gaaagctctt cccaaggaca acccgaacat tatggtttac gatacaatct gtcacgccac
     5461 acaggtgcgg cagtccgacg cagacaaact ctctcgcaag tcagatttaa tgattattgt
     5521 gggcggcaga cacagttcca acaccgtgaa actgtatcat gtctgcaaca gaaactgtag
     5581 gtcttatcat attgaaaatt cggatgnnnn nnnnnngcac atatcattaa ggaggttcaa
     5641 caaactatga ctgaaatcat ggataacaac gccgtggagg aggatatcaa cttcgcagaa
     5701 gctctggatc agtccttcaa aaaaatacat acgggagaga aggttaaggg tactgtttgc
     5761 tctgtaaacg attctgaagc gatcgtagac gtaggcacaa agcacaccgg ttatgtaccc
     5821 cgcagcgagc tgaccgatga cgcttccaag aagccgtctg acgtagttgc tgtcggcgat
     5881 gttctggatc tgatcgttac caagatcaac gaccaggaag gtatcgttac cctgtccaag
     5941 aagaaggttg acgagatgaa gggctttgag gaaatcatca aggccaagga tgaaggcgca
     6001 gttctggaag gcactgtaaa cgcagctgtc aagggcggcg tgatcgtgac ctatgcaggc
     6061 gttcgtgtat ttatcccggc atctcagagc ggtctgccga gaggcgcaga gatggattct
     6121 ctggtaaaga ccacacagcg tttcaccatt ctggaagtca acgaacacag aagaagagct
     6181 gtcggctcta tcaaggctgt tctgaaggct gaaaaggatg ctgccaagga agcattctgg
     6241 gaaactgctg agatcggcaa gacctataag ggcgaagtaa agtctctgac cagctacggc
     6301 gcattcgtag acctgggcgg catcgacggt atggttcaca tctccgagct gtcctggaag
     6361 cgcatcaagc acccgagcga agttgtcaag gttggcgact tcatcgaagt ttacatcaag
     6421 gatctggaca aggaaaacaa ccgcatttcc ctgggctaca agaagcagga agacaatccg
     6481 tgggagaagt tcaaggcaaa ctatgctgtt ggcgatgtga tcgacgcaac catcgtttcc
     6541 atcactccgt tcggtgcatt tgcacagatc attgacggcg tagacggtct gatccacatt
     6601 tctcagctgg ctgacaagcg tgttgaaaat gtaaaggata tcgtttctgt cggcgatgta
     6661 gtcaaggtca agatcattga catcgacatc gacaacaagc gtatctccat ctccatgcgt
     6721 gcgctgctgg aagatgagga cgatgcagat tacgaggact aatttccccg taagcattat
     6781 aattgcataa aacaaggcgg cagtaccggg tttccggcgc tgccgctttt ttgtggatgt
     6841 atcataaaaa tgaatacttg ggcggcgata aatgccgac
//
LOCUS       DAWENO010000110         6814 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4504_length_6814_cov_10.100607, whole genome shotgun sequence.
ACCESSION   DAWENO010000110 DAWENO010000000
VERSION     DAWENO010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_6814_cov_10.100607"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    699..708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4015..4024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1793 a         1712 c         1669 g         1620 t
ORIGIN      
        1 tttgtttcag aatttctgca ccttgaacca tcccgcagtt tcgctgcaaa taccggaaaa
       61 aagctttccg gtatttcata atttttttgc ctttttattg acaattcaac gattctatga
      121 tataataaag gcaaatatat tttatatatc atgtccaata taccatgaaa gagggaaaag
      181 atgaaaaaac agatcatttc ttgtgcagca gtactggcaa tgctggggac ggcagtctgc
      241 gtcccggaaa gcagtatttc ctgccttacc actctgtcgc tgcatacagc agcggaagag
      301 accgacaccc cggatgatgc ggcgacagaa agccaggtgt acgacacttc cacacttaca
      361 ctcggcacaa agctttccca gggtgacacc ctgcactatg acaaacaaaa catcggcagc
      421 gtggcaaata tcgtcaatgc caaaggcgat tatgaccttg catttatttc tgaagaggac
      481 tatgttctgc cctttgatgc cgaggttgtc ggaatcactg cgctcaccat ctatcttgcc
      541 ccggcagtgg acggcattgc ttataccgat gtacgaaccc tttccgacgg ggacaccatc
      601 gacagaaata cgaacctcct ctgctacgac tacgtcatca acaaccgggt gctgccggta
      661 tttctcccat cctattatga caagtacatc gggacaggnn nnnnnnnnca ccattcgggt
      721 gaaatccatc gaccacgaaa acaagcgcat tcaactggaa tccgtagaca gaccggaaat
      781 ggtaaaaggg gatgtcaacg gcgacggcat tgccgacgtt tccgacattg tccttttcca
      841 gaaatggctg ctgggcgacc cggatgcagt gctttccaac tggaaagctg ccgacctcta
      901 tgcagacggt cagctagatg caatggatct gctgctgatg aaacagacgc tggtcagaca
      961 ggaacagatg gacaccaccc ctgccctgct catccagaat tatgagatca atctgtcaga
     1021 gctgggctgg atcggcgaaa acaccgacca tctgattact ctggacggtt tccgctattc
     1081 tctggctgcg gaaaccgatg ccatcgacgc tccggcaggc gattataccg aatcgctctg
     1141 ctccaacggc atccaggact tgttcgtcta cgacgaaagc gttttgcagg aaatcgcaga
     1201 ctttgcaaag tctgctgaaa agtacaaaga ctgcgccatg aaaagctgga attttaacat
     1261 agaggactac ggaacagaca cactgtacgt gctgtatacc gattcctccg gcgtgatgca
     1321 aaaactcgcc ctctgtcaga tcggcggcga atgtgcatgg ctggataacg aagaggtaca
     1381 ggcatttgtt acaatgctga tcgaaaaggg gtatttctca gacaagtcca atttggacgc
     1441 ctatctgtat tataaaacag cataaaaaca aaatcggtca gagattccct gcggattcct
     1501 ctgaccggtt ctatttttcc aaatggctgg actttacagt tctttcttat tgtctgcacc
     1561 cggtgttgtg acgatcacct tttccggtgt gatgagcagt gcgcccttgg cagatgctgc
     1621 gccgtggaac atcgtttcca ccatctcccg gaacttttcg acgatctcgc cctgggtgag
     1681 gtattccgct gtacctttga tctggtatcc ctccaggctg gacgcatcat acacagaaac
     1741 tgcgattttt ccgttatttt cccgaagatt ccgcagcgtg gtatccagga aaacatcacc
     1801 taccgccagc tttccgtctt ttgtcacaaa cttgaatgct acgggaacca cgttgggttc
     1861 gccgtctgca caggttgcca gatcccacat acttgtttca agtacctttg ttacattctc
     1921 attcaacatg atagattcct cccggttcat tttctttgat ttacgcattt tcctctgcgc
     1981 tgcctttatt atagcaagac tgctttccgg aaacaagata ttagtcaatt tataagtcac
     2041 tcatcaattt catagttact tgaaatccaa aagtatctgt ggtatcataa agatatccgg
     2101 aaaacacgaa aaaggagttg gaatcatgta tcagcgaaaa ttggaaaaag acatccgctg
     2161 tccgctggaa tacgggctgg aggttttcgg cggcaaatgg aattccagga tcatctgcgt
     2221 tctggcagcc aagaaagtgc tgcgctacag cgagatccga aaggaaatgg gcaatatcac
     2281 cgatgcggtg ctggcatcct ctctgaaagg gctgatcgcc aacgacatcg ttacacgaaa
     2341 gtcctacgat gagataccgc cccgtgtgga gtattccctc tctgaaaaag gcatctctgt
     2401 tgttcccatt ctgcaaagca tctgcaaatg gtccggtgca ttttataagg aagacccgga
     2461 gcatatgatg atacactgtc aaaaatgtga ttatcaatga aagaccattt ccggcaatgc
     2521 cgcacttctc tgttcggcat tgccttttct attttctgcc gcttgacatc tgctgcaaag
     2581 tgtgatataa taaacctgta aattcacaca agttcactat ggaggaaaag aaatgaaatc
     2641 ctttccggaa aaaatcaaag aggcaagaag tgcgctttcc atgtcccagg agacgcttgc
     2701 cgaaaaggtc ggcgcaagca ggcgctccat catcgcctat gaaaaaggag acaagcatcc
     2761 acgggaccgg atgctgtatc agctggcaaa ggcactgcac gtttccatcc gatatttgaa
     2821 agatgacacc tgtgaaaatc caagagaaga tatcgaaaag gatatttaca tcatggagac
     2881 acaaaatgaa tatggtgcca gagatgcacg gaatgttgcg gcacttctgg cagaaaacca
     2941 ggcgctgttt gccggcggcg agctgtctca gacggaaaaa gacctgttct ttgaggcgct
     3001 gatgacggca tacgtcacca gcaagcagca ggcaaaagaa aagtacggca aaaagaaaag
     3061 ctgaaaagtt ggaggaaaaa tcatgctgac agtaagcagt gatgaaattc gatgccgggt
     3121 cgaaaagctg cggcgacagt ttcagacatc ggatccgttt atcctgtgcc gacagctgga
     3181 tgtgcagctg aaaggtgtga actttggcga aacagaacac tccatcaaag caatgaccac
     3241 agtcattttc cggatacgga tgatccagta caacagcaat ctgccccgga cactgctcac
     3301 ctttatcctc gcccatgagc tgggacacat tctcctgcac agccgggaaa tcaaaacatt
     3361 ccaggagacg gaaacaacgc tgtacgaaac caaccgcctg gaaactgagg caaaccggtt
     3421 tgccgccgag ctgctgctgg gtgacagtgc atccttttac cgggaaatgc aggattccga
     3481 ctatacgctg ttccagatcg ctgcggtgca tcatgtcccc tacgatttcc tggcttacaa
     3541 actgcaaatg atggaagaag agggttatcc cgttccagaa ctgcccgcag agccggacag
     3601 ccgctgtctg ggcggtgatc tgggactgcc ggactgctat ggcttttacg aagagtgagg
     3661 tgcggacaat ggcatcctat atttgcattg atctgaaaag cttttacgcc tctgtggagt
     3721 gcgtggatcg tggactggat ccgctgaaca cgaatctggt ggtagcagac accggcagaa
     3781 cagaaaagac catctgtctc gccgtaacgc ctgcgctgaa aaagtacggc attcccggaa
     3841 gagcaaggct ttttgaagtg gtggagaccg tccggaatgt gaatgcgcag cgtctgcgga
     3901 aagcgccttt tcacgagttt acggggagtt cctgccaggc atcagagctt gcacaaaatc
     3961 cggcgctgaa actggatttc atcgccgcca agccccagat ggcacactat atggnnnnnn
     4021 nnnngagatc atcacaaaga tctaccagat ctatctccgg tatattgcgc cggaagatat
     4081 ccacgtctat tccattgacg aagtctttat ggacgtgaca ggatatctga aaacctatca
     4141 ctgcacgccg cacgagcttg ccatgaaaat gatacaggcg gtactgcgtg aaaccggcat
     4201 cacagcgact gccggcatcg gaacgaattt gtaccttgcg aaaatcgcca tggacattgt
     4261 ggcgaaaaag atgccggcgg atgcaaacgg cgtccgcatt gcagagctgg acgaaatgac
     4321 tttccgggaa aagctgtggg atcacaaacc gctgacggac ttctggcgga tcggcggcgg
     4381 ctacgccaaa aagctggaaa agatgggact gtacaccatg ggtgacattg cccggtattc
     4441 ggaacacggc gagcgagcgc tgtttcagat gttcggggtt gccgcagagc tgctcatcga
     4501 ccacgcctgg ggatatgagc catgcaccat gaaggcaatc aaaacgtacc agccgaagga
     4561 tcacagcatc agccagggac aggtcctgcc ctgtccttat gactggcaaa agggcaggct
     4621 tatcattcgg gaaatgacgg agctgcttgt gctggatctg gtgcgaaaaa aactggcagc
     4681 cgaccagatc gtgctttcgg tgggctatga ccacacgggc atcccggaaa actatcaagg
     4741 ggaattgaac ctggatcgct acggaaagcg cattcccaag gcagctcacg gctctgtcaa
     4801 tctcggaaag cacaccgcct ccactcgtct tatcctggaa aaagtcacag cactctacga
     4861 ccgcattgtg gatgcggaat tgctggtgcg gcgcatgaat gtgacggcaa accatctgat
     4921 cccggagcat gaggttcagg aagaggtggt acaatacagc ttattcgacg atgtacaggc
     4981 gcaggaacag cggcaaaagc aggaaaaaat ctttttggaa aaggaacacc aattgcagaa
     5041 aaccatgctg cgcatcaagg agcgatacgg gaaaaatgcc attctgaaag gcatgaattt
     5101 ccgggaaggt gcgacaacga tagaacggaa cagccaggta ggagggcatc gggcatgaac
     5161 gattacagcg atatccgcag ccatgaaaga taccacttga aaaaccacat tcccatgccc
     5221 atggaaaatc gtgccgtgca gtttgcgcct ttcgctgcgc tgacgggcta cgatgaagaa
     5281 attcacgagg cggcacgtct gacagaccct atggaaaacc tgtcagacga tgcacttgcc
     5341 accttaaatc tgcatttcca acagcttctg gaacacgccg cagaacgtcc tcgggtacgt
     5401 gtcacctatt tccagccgga ttccaaaaaa gacggcggcg catatgcggt atacaccggg
     5461 tgttttcgat tttttgatgc agaaaaacga tgtctcaagt tcacgaacgg cactgcgatt
     5521 tctgcggaac gggtgtgcag actggagttt ctgtccgcac aggcataaaa acaaaaactg
     5581 cacaatggga atcacacccg ttgtgcagtt ttgcatatat tttggagtat atgatttgaa
     5641 ttgtgtttgg tcaggagtta tccccagact tcttcagcaa tctcctttac cagcgcaagc
     5701 tttgcccatt cgtcttcttc ggtcagctta ttgccgatct cacaggacgc aaatccgcac
     5761 tgcgggctga ggcagagacg atccagcgga atgtactttg cagcctcatg gattcggtcg
     5821 ataacggtct gcttgtcctc cagttccggc tttttagaag taatcagacc cagcactacc
     5881 tttttattgg gagaaacctt tgcaagcggt tcaaaaccgc cgcttctctc atcatcgtat
     5941 tccagcagca gtgcgtctac attttcctgt gcgaaaacat agtctgcaac agaatcatac
     6001 gggccgcttg taaagaatgt ggagtgatag ttgccacggc aaatgtgcga agtgattacc
     6061 atatctgccg gcttattctc cagcgccagg ttgttgactg ccaggagctg tttcttgaca
     6121 tcttccaggt caatgccgag cgactgataa cgctgctttg cggcatctcc cacgattgcg
     6181 ccccaggtgc agtcatccag ctgcaaattt ctgcatcctg catcatagaa ctggtggatc
     6241 acattctgat atgccagtcc gatgtcctgg atcagttctt cgtttgttgc gtagaattct
     6301 ctggtactct ggaaattctg cggaacgatc atctgctgga acatctgtgc cggtgccggg
     6361 atggtgtact ttgcaacagt ctcatcgtcc tcgaactgtt tcagaaactg aaaataggta
     6421 acaaacggat gcggtactgc cttgaccttt ccggtcagat aggtatcctc cagcgatgcc
     6481 agctcgccgc tgaactgtac gccggcgcct gtctgctcat gtcctacgcc ttcaaagccc
     6541 cacatgaaat ccagatgcca gtattctcgt ctgtactcgc cgtcggtgat gatgtgatag
     6601 cctgccttct tctgcttggc aaccagttca cgaatggcat catcttctac tgcggtcagc
     6661 tgttccttgc tgatttttcc agccttgtaa tcctttcgag cctgcttcag actctccgga
     6721 cgcaggaaac ttccgacaaa gtcatagcga taaggcgatg tgatcttctg cataatcggt
     6781 acctctttct tttcaatggg attttctctt cttt
//
LOCUS       DAWENO010000111         6813 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4506_length_6813_cov_9.810891, whole genome shotgun sequence.
ACCESSION   DAWENO010000111 DAWENO010000000
VERSION     DAWENO010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4506_length_6813_cov_9.810891"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    468..477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1555..1564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1820 a         1543 c         1591 g         1839 t
ORIGIN      
        1 aaacccgatc tggcacatta ccaaagcaat cacacaggta atgtagtgct ttgccgcaaa
       61 tccaacgccg ccgaaacagg attgaaatat tacgctgata ttgacaagca ggagcagcac
      121 gaaatccagt gtgtaggtca tttgtcggta cataaaatgc agtacaatgc tgtatacaat
      181 gtccagacca atcacggcta cagtgagcgg cagaatatcc tgcggtgtgt cataatttcc
      241 acgcagcaaa acagcgagca gcatgatcgt atgcgtgagc aaaacaatgg cagtgcagct
      301 gccgtaacga agtccatttt tatacattgc gcactcccct tcttctttgt cgaattacga
      361 cagattttgt tccaaatcgg atctcatcca tatcttttaa ttttacttgt gagcggattt
      421 ttcttccatt tacataagtt ccggattttg agccaagatc catgacannn nnnnnnnaca
      481 cgccgtttga gacgtataac acagcgtgat accgtgagac gctcatatct gtaaagcgga
      541 tatcagctgc accgtggcgg ccgacaagga tctcatctgc ctccaatgga atgacgactc
      601 ttctggcgtt atcatacaat gccatgtgcg cactgacctt gtcccaccgc agctgttcaa
      661 tttctttttc ctgccgtgct tttccgaaca ttgtcaggac gctgacagca atgaatatga
      721 caacaactgc actgataaag atagaatatg ccggtatctc ctgcagggaa gtcagaagtt
      781 tatcaagcat tataaaacct cctgtcattt gattttacat aaacatcata attgattttt
      841 tgaaaagttg caagtaaaat agcttgcttt tgtagaaaga tacaaatgat tgccaatatc
      901 aatgatcgaa acatagcaag atgcctaaaa gcgaaattaa attttgtgta tgttcaacaa
      961 aattcaaaaa tgcacttgcc ttaaaactag aagtatgtta taatatgaga aacaaacagt
     1021 tccatagcaa tcagcatagc tgactgtatc gggctgtaga aggaggaatt catcatgaaa
     1081 attacaatta cagcgaagaa aatgcagatc ccacagaact ttacagagta tgctgaaaag
     1141 cgtctgagcg caaagctgga caagtttttc ggcagcgaag cagaagcaaa aatcacaatg
     1201 gcaacacaca agaacttaat tgttattgaa ttgactgtta cctataataa tttgatttat
     1261 cgtgcagagc agtctgctgt agataaggaa gatgcactgg atgcttccat tgataaaatc
     1321 attcgtcaga tccgaaagaa caagacccgt gtggaaaaga aactgaaaga ggctgctttc
     1381 atgggaatga tggaagagcc tgttgcagag gcagatcatg aagtaatccg tcacaagaaa
     1441 tttgtgatgc accccatgga tctggatgag gctattcttc agatggacct gctgggacat
     1501 tctttcttta tgtttaccaa tgcaaatacc ggcgaagtaa atgttgttta caaannnnnn
     1561 nnnncaaacg tgctgacggc aaatatgctg ttctggagcc gatttacgag taaacacaac
     1621 gtgaaaaaat actggaatat agaaagcgga tactgcgatg cggcggtgtc cgctttttgt
     1681 attctcagat tctctgtgcc agacgttcgc tgtagtcttt ccggaactgt gcaaattctc
     1741 cggcatccag agcgtcacgg atgcgttcca tcagcgtatt gtaaaaatac agattgtgca
     1801 ttactgccaa acggccagcc agctgctctt ttgccttaaa caagtggcgc acatatgcac
     1861 gggaatgatt ccggcatacc gggcagtcac atttcggatc cagcggctga gaatcagttt
     1921 catacgcagc gtttgtgata tgaagaatgc cgtcccatgt gaatatggtg ccgtgacgtg
     1981 catttcggct tggcataaca cagtcgaaca tatccactcc ccgagcaaca ctttcgatga
     2041 tgttagaagg cgttccaacg cccatcaggt agcgcggctt atcgactggc atatgcggct
     2101 cgacttcatc aataatgtga tacatgacct ctgtaggctc tcctactgcc agcccgccga
     2161 ttgcataacc gtccagtccc agttgtgcga tttcctgcat atgagaaaca cgaattgcat
     2221 cgtatgttcc gccctggttg atgccgaaca gcatctgatg cggattgatc gttccatcca
     2281 gactgttcag acgctgcatt tctttttgac agcgaaccag ccagcgagtg gttctggcac
     2341 aggaattttt cacataatcc agcggtgccg ggttttccac gcattcatca aacgccatgg
     2401 caatggtaga gccaagattg gactggatct gcatactttc ttccggtccc atgaagatgg
     2461 ccttgccgtc aatatgagac tggaaatata ccccttcttc tttgatgcgc cgcagttttg
     2521 ccagagaaaa cacctggaaa ccgccgctgt ctgtcaaaat cggcttgtgc cagttcataa
     2581 actgatgcag tccgcccatt tgccgtacga ttttatcacc cggccgcagg tgcagatgat
     2641 aggtattgca taattcgatc tggcatttca gctgatctgc caggtcaatg gaggatatgc
     2701 ctcctttgat agcggcagca gttcctacat tcataaaaaa cggtgtctga atcgtaccgt
     2761 gaacggtttc aaatacgcct cgtctggcgt gcccttcggt ttttaatagt gtaaacatct
     2821 ttcgtataaa ttcctttctg ttttatcctt gtgtcataat cggaggagtt tgcagtaatg
     2881 tgcagatctc tttagcaaac gctgctataa aggcttcttc ctcttcttcc atggaaaaat
     2941 aagatgcaaa tatactttta tcttgcatca acttttttcg cagagaaacc gcccatgctg
     3001 caccagattg aattttggaa gtgccagcag caaaaccacg caaaatatcc agcaaatctt
     3061 ctgtcagtaa cgcactgtca gttgtcttta gacaatacag caaaatgtca taagcaggaa
     3121 gtgcagcatc tgcaaccata atatgttgat ggcaaagtgc attatctaat tttaatgctg
     3181 cttcccttgc gtccatttcc tgccctgcaa aaagcagttt cagccatttt gcgatatctg
     3241 cctgatctct gcaatcctcg tcaaaatatc tgtgacggtg cattgtcatg gacatcgaaa
     3301 gaaatctttc ccaccatttt ttcggattgg tttcaatccc ggatgcgttc ccatatgctg
     3361 tatgatattg tgaccagtca attgcgtcaa ttcgtgcgaa aatctcctgc agttcttctg
     3421 gtgaatgtga tttcattcgc ttttttccgg taagatacac atggcatcac caaagctgaa
     3481 gaaacgataa cgttccttga ctgcaatttc ataggcgtgc atggtctttt cgtatcccag
     3541 aaatgcagag attagcataa ttaaagtgct ttccggcagg tggaagtttg taatcagacc
     3601 atccagtacc ttgaatgaat agccgggata gatgaaaatg tcagtatatc catcgtcagc
     3661 caccagttcc ccattgtgag cggttgcaac tgcctctagc gttcgacaag aggtggtacc
     3721 gacagcaatg acccgaccgc cgtttgcctt ggtttcacgt atcatatcta cagtttcctg
     3781 cggcagatga taatgttcgc tgtgcatctg gtgctgcaat acgtcatctt cctttaccgg
     3841 gcgaaacgta ccgagtccaa cgtgcagtgt gacatacgcc tcttttaccc ccttttcttt
     3901 tagctttgcc agcatttctt ttgtgaagtg cagtccggct gtaggtgctg ccgcagagcc
     3961 gagttccttg gaataaacgg tctgatagcg ctctttgtct ttcagtttct cgtggatata
     4021 cggcggcagc ggcatttgtc ctacttcttc caaagcggtg tagaaatttc cctcacaggt
     4081 gaactgtacg attcggttgc cgtctggttt tacttcaaca acttcgccaa tcaatctgcc
     4141 gccgccaaag gagaacttca cgccgacctt tgcctttttt ccgggacggc agatgatctc
     4201 ccacagcttg tcccccttct gctccaacag cagaaattcg atgaatgtac cgttgtccac
     4261 tttttcaccg tacagacgtg caggcaaaac acgggagtca ttcatgacga gcaaatcccc
     4321 cggctgtaag atgtccagca gatcataaaa atgcttatgt tgaatctctc cggagtttct
     4381 atcaatttgc atcagtctgg agtgatctct gggttcgatt ggcgtttgtg caatcaattc
     4441 ttcgggcaaa tcatagttaa aatctgattt tttcataggg ttctcctctt tttcgcaaaa
     4501 tgttattgac aaattcccac gaaaatgata tgataggaat aggtagaggc gcacttgcga
     4561 tgagtatgtt ccgacaggtt accattccgc aacgatcggg gcagaaaggc gttcgtgccg
     4621 aagggactgt accggtgaca cagttcctgg gcatatttcc gaacaggagt atgactgtca
     4681 tcgttatgat ggagtgctat cggcgatata ccattcggtg tacgtcattc tattctatta
     4741 tatcgcatga gccgccggtt ttcaaccggt tttttcatat ttttaagaat tttttggagg
     4801 aatgacgaaa atgaaacagt acaaggtcgg aattatcggc gcaacaggta tggttggaca
     4861 gcgatttgcc acacttttgg agaatcatcc gtggtttcac gttgctgcgc tggcggcttc
     4921 tccccgttcg gccggaaaaa cctatgaaga ggcagtcggc aaccgctggg caatgcagaa
     4981 gccgatgccg gagtctatga aacagatggt tctgtttgat gcaactgcag atgttgacaa
     5041 gatcgcaggt atggtagact tcgtattttg tgccgttgac atgaagaaag atgagatccg
     5101 tgcgctggaa gagcgttatg caaaggcaga atgccctgtt gtttccaaca actctgcaca
     5161 tcgtttcaca ccggatgttc cgatggttgt gccggaagtt aacccggaac acattgagat
     5221 catcgaaagc cagcggaagc gcctgggaac taagcatggt ttcattgcag taaagtcgaa
     5281 ctgctcgatt cagagctatg ttccggcact gtctcccctg cggaagtatg gcatcaagaa
     5341 tgtgcttgcc tgcacatatc aggcaatttc cggtgccgga aagacatttg agcgctggcc
     5401 ggaaatcctg gacaatattg taccgtatat cggcggcgaa gaagaaaagt ctgagaagga
     5461 accgctgaag gtttggggca agattgaaaa tggtcagatc gtcagcgctg acagccctct
     5521 gattacaaca cagtgcctgc gtgtaccggt ttcggacggt cacactgccg ctgtatttgt
     5581 ttcctttgag cagaaaccgt cgaaggatga aatcctgaag atctgggaag agttcaaggg
     5641 cgttccgcag gaattggatc tgccgtctgc accgaagcag ttcatccatt atttccagga
     5701 agacgatcgt ccgcaggcaa agctggatcg caacattgaa ggcggtatgg ctgtttctgt
     5761 tggccgtctg cgtgaagaca ctatgtttga ctataagttt gtctgcctgt cccacaatac
     5821 cctgcgtggt gcagccggcg gcggtgttct cttggcagaa ctgctcgcag caaagggcta
     5881 tatggactaa gtttatttga tacacaattg atttgaatta ccattaggag gtaattgcta
     5941 tgaaaaacac catttttaca ggtgcaggcg tagccattat tacgccgatg ctggaagacg
     6001 gttctgtaga ttatgcagga ctgggcaaga acattgattt tcagattgaa aacggcacag
     6061 atgccattat catttgcggc acaaccggtg agtcctccac gctggatgac aaggaacatc
     6121 gggagtgtat ccgatttgca gtagagcata cccacaaccg gatcccggtg attgccggaa
     6181 ctggtagcaa cgatacgaag tatgcgattg aactgtctca ggaggcgcag gaactgggcg
     6241 cagacggtct tttgattgtt accccgtatt acaacaagac gacacagcgt ggtcttgtag
     6301 cccattataa cgccattgca gacagcgtaa atctgccgat tattctctac aatgttccca
     6361 gccgtactgg tgttggcttt gatgtgaaca ctgtcgcagc acttgcagag cataagaaca
     6421 ttgctgcaat caaggaagcg agtggtaata tcggttatac tgcaaaggtt gcagcaaagt
     6481 gcggagataa gatcgacatt tacagcggca atgatgatat gatcgttccg atcatgtctc
     6541 tgggcggtaa gggcgtaatt tcggtactgt cgaatgtttt gccgaaggaa acacatcaga
     6601 tgacacagta ttgtctggaa aataattttg cagcagcagc ggaaatgcag ttgaagtatc
     6661 ttcgcctgat taacgacctg tttatggaag tcaatccgat tccggtaaag gacgcaatga
     6721 atcagctggg aatgccctct ggtccgtgca gaatgccgtt gctggatatg agcgatgagc
     6781 ataaggctgc aatgcaggca acactccgtg cat
//
LOCUS       DAWENO010000112         6736 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4565_length_6736_cov_10.608592, whole genome shotgun sequence.
ACCESSION   DAWENO010000112 DAWENO010000000
VERSION     DAWENO010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_6736_cov_10.608592"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1785 a         1533 c         1914 g         1504 t
ORIGIN      
        1 tgttctgctg accctggagg caacagcaat tgcagttggt ttcaatacca tattcggtat
       61 ttttgcggcg ttggctgtga cgaagttcca tttccgtgga aagaaacttc tcaccacgct
      121 gatcgacgtg ccggtcacca tttcaccggt cattgctggt ctgatctttc tgctgacgtt
      181 cggcagaaag agcgtgatct atccgttgct ccacgacctg ggaatccggg tagtgtttgc
      241 ggtgccaggc attattctgg ctacggtatt cgttacgttt ccatttgtct cccgggaact
      301 gattccggtg ctggaatccc aggggacaga tgaggaagag gctgctgcgc tgatgggtgc
      361 aaacgcctgg acgattttcc gaaaggtgac ctttccccat atccgctggg cgctgctcta
      421 cggcgtggtg ttgtgtgcgg cgagagccat gggagaattc ggtgcggtat ccgttttgtc
      481 cggtcacctt cgtggcagaa ccaacacact gccgctgcac gttgagatct tgttcaatga
      541 attccagtat gtaccggcgt ttgccgtttc atccattctg gtcatgatgg caattgtgat
      601 cctggtgatc cggagtctca ttgaacaccg aggaaagagg gacatctgat gtatgtagaa
      661 ctgaaccata ttcaaaagcg tttcggcaat taccaggcgt ccgatgatgt cagctttggc
      721 attgaaaagg gaaagctgat cggtctcctg ggtcccagcg gaagcggcaa gacaaccatc
      781 ctgcggatga tcgccggtct ggaacatccg gacagcggcg agatcaagat cgacgggaaa
      841 gtggtcaaca atatcccggc aagcaagcgt ggcatcggct ttgtgttcca gaattacgca
      901 ttgttccggt acatgaccgt gtatgacaac attgcctttg gcttgaaggt ccagaaagcc
      961 gaccgcaaaa ccattcacag ccgtgttatg gaactggtgg atctgatcgg tctgcgtgga
     1021 ctggaacggc gttatcccag tcagctttcc ggcggtcagc gtcagcgtgt ggcattcgcc
     1081 agagcgcttg caccaaatcc gcagctgctc ctgctggacg aaccgtttgc cgccattgac
     1141 gcaaaggtgc ggcaggaact gcgaacctgg ctgaaagaga tgattagcaa gctgggtgtc
     1201 accagtattt tcgttaccca tgaccaggac gaggcaatcg aggtagcgga cgagatcatc
     1261 attaccaatc gtggcagaat cgaacaggtg ggaacgccca tcgagatcta ccgtcatccc
     1321 cagacttcgt ttacggcaac tttcttcgga catacttccg tgctggcaga ctatcagaag
     1381 tatcacacct tccaacatat ggacggcgtg gaaaaggcaa tcgtccgccc ggaatttctc
     1441 aagatcacca agaaaaacga ggtgcagaaa tacggcagtt ccgcatccca gggcgtggtg
     1501 gagcgtactt cgttccgtgg cgactggatc gaactggaag ttcgtgtaaa cgacgatctg
     1561 ctgattgcca agcgtgatct ggaagaggaa agcgtttcgg tgggagaaac cgtggacgta
     1621 ttcctgtatc gtctgtttgt gatcgtggga gagaaatgtt atctgttgga gaaccagtct
     1681 ctccaggacg attctgtatt tatttgattt tcgttgtcaa tacgtgattc gatattatta
     1741 tgaaaaaagg agttatgcgt tatgtctaag atttatcaag gtgcagtgga actcatcggc
     1801 aataccccgc tggtagaagt catcaatctg gaaaaggaac aggaactgaa agcacgtgtg
     1861 ctggtcaagc tggaatatct gaatccggca ggcagtgtca aggatcgtat tgcaaaagcc
     1921 atgctggaag acgcagagaa gaacggaaag ctgcatgagg gttctgtgat tatcgagccg
     1981 acttccggga atactggtat cggactggca gcgattgccg catccaaggg ctatcgtgtg
     2041 atcttgacca tgccggagac catgagcgta gagcgccgca acattctgaa agcatacggc
     2101 gcagagattg tgctgacaca gggcgtgaaa ggcatgaagg gtgccatcga gcgtgccaag
     2161 gaactggcgg aagagattcc cggtggcttt attccgggac agtttgaaaa cccggcaaac
     2221 ccggagacac accgcagaac gactggtccg gagatctggc aggacacaga cggtgaagta
     2281 gatttcttcg tggctggcgt tggcacaggc ggtacgatca gcggcactgg cgaatatctg
     2341 aagtctcaga aaccggatgt aaagattgtt gccgtagaac cggcagattc tccggtgctt
     2401 gccggcggaa agcccggccc gcacaagatc cagggcattg gcgcaggctt tgtgccgcag
     2461 acactgaaca cgaaaattta tgatgacatt tttgaggtca agaacgatga tgcctttgcc
     2521 gcagcaaagc tgatcgccaa gcatgagggt atcctggtcg gaatttcttc cggtgcggca
     2581 ctgcacgcag cgctggaact ggcgaagaaa ccggaaaacg caggaaagac cattgttgca
     2641 ctgctgccgg acagcggcga ccggtattat tctaccacat tgttctcgga ttaaatcctt
     2701 ttattcacag gctttttgtg aataaaagtt atagccagac tctgaccagg aggaggagta
     2761 tcattgaaaa tttcaaccaa agtcgaatgc ggcatcattg cactgattga cattgccatt
     2821 cactctgaaa acggagaggc ggtggctgtc agcagcattt ccaagcggca gaatatctct
     2881 gtcaaatacc tggaacagat cctggtggcg ctgcggcaga cacatctgat ccggggcatc
     2941 aagggattca aaggcggcta tgtcgtcgca cgcccggcag atcagatctc attccaggag
     3001 atcattgatg cactggatat caccatcctg ggggatgtgg atgccggcgg tgcagatgat
     3061 acctcccttc tgaaagcgac gatccaggaa tctctgtggg acaagatgac tgcttatctg
     3121 cggcagttct gtaccggcat tacattgcag gatatgatgg accgctaccg cagtgcgatt
     3181 ccgcaggatg aggcatttat gtactatatc tgaccggtgc tgctgtaagc agtatctgac
     3241 agcggacaaa actcccagcg gaggagtagg aatatcggct gcgttttctt tcaatcagac
     3301 ggcatccaat cagattttgg atgaaccgtc tgatttttac cgcaaaatca gtgaatattt
     3361 cattttgaag aaaacgaaac aatgaacaat atgacgaaaa tgaaaaagtc gaataaatct
     3421 attgacttac tagtgaaaag gtgatataat aagctcatga caagggaaca gaagaaaaat
     3481 gttccgaaga acgagaggag cgtgggtgag cgcatgaagt ttaatacggc attgttgcat
     3541 cagaattttg atggagaaaa gagtacaggt tctaccatca tgccgattta tcaggtcagc
     3601 gcattcgcac atgagacggc agaaaaactg gaaaaagttt ttcataatca agcgccggga
     3661 tttgcatata ccagaatcag caacccgaca gtagatgctt ttgaaaagcg agtggcggca
     3721 atggaacagg gaatcggcgc agtgagctgt tcctccggca tgacggcagt caccatggca
     3781 ctgttgaaca ttctccacgc cggagacgaa gtaatcgccg gttcgggact gttcggcgga
     3841 accatcgacc tgttttcgga tctgaaagct ttcggcatca ccacacgctt tgcagagtcg
     3901 gtaacggtcg aagagatcga accgctcatc aatgaaaaca ccaaggtggt tttcacagag
     3961 ctgatcggca atccgaaact ggatgtggtg gatctgaaag cggtctctga actggttcat
     4021 gcacacggca ttccgctgat tatcgacagc accaccgcaa cgccgtacct catccatccg
     4081 tttgcatacg gcgcagatgt ggtcgtgcat tcctcgtcca agtacattaa cggcggcggc
     4141 aatgccatca gcggcgtgat cgtggacagc ggaaacttcc actgggatat gagccggtat
     4201 gacggatttg aaccgtatgc caagtacggc aagtttgctt atctggcaaa gctccggaat
     4261 gccatctggc gaaatgtagg cggatgtttg tctccgatga atgcgttcct caactccgtg
     4321 ggactggaaa ccttgggact gcgtatggag cggctgtgtg acaacgcatt gcagctggca
     4381 aagttcctgg acagcgtgga cggcgtggaa gtcaactacc cggcgctgga aaagagtccc
     4441 tattatccgc tggtgcagaa acagttcggc ggcaagggcg gtgcaatctt gacgctccgt
     4501 gccgggagca aggaaaaggc attctgtctg attaaccatt tgcagtatgc caccaatgcg
     4561 acaaacatcg gcgatgtgcg gacgctggtc atccatccgg caagcaccat ttacgcacac
     4621 agcaatgaaa aacagaaaca aagtgccggc gtgtatgagg attccatccg catcagcgta
     4681 ggcattgaag atattgaaga cctgatcgag gacttcggac aggcaatcac gaagatgcag
     4741 gaggaatgtt aaatggcagt tgattatgcg accctaaaaa agggcggatt catgcggcag
     4801 aaacaaaaga ataacttttc cctccggctt cgtgtggtcg gcggcaatct taccgcaaag
     4861 cagctggcaa agatcgcaga ggttgcggaa aagttcggtg acggacacgt acatctgact
     4921 tcccggcaga gcgtagagat cccgtttgtg aaactggatc agatcgaaga ggtcaaggca
     4981 acgctggctg agggagatgt agaaccgggt gtctgcggac cgagagttcg taccattact
     5041 gcctgccagg gcgaggcgat ttgccccagc ggctgtattg atacttatgc gctggcaaag
     5101 gaactggatg agcggtactt tgcacgggag ctgccccaca agtttaagtt cggcatcacc
     5161 ggatgtcaga acaactgctt gaaatccgag gagaacgacc tgggcatcaa aggcggcatt
     5221 caggtgaaat ggcgtgaaga ggactgcatt cagtgcggtg tctgcgtcaa agcctgccgc
     5281 agcggtgcga tcacccatga gggggataag atcgtggtag acgaaagcaa gtgcaacttc
     5341 tgcggacgct gcgtgaaagc ctgtccgaca gaggcgtggg acacggtgca tggctatatt
     5401 gtttccttcg gcggcttgtt cggcaacagc atccacaagg gtgaaccgat cattccgttt
     5461 attgcagaca aggaaaagct gatgaaggtc tgtgatgcga ccgtgcagtt ttttgacgac
     5521 aacgccaagg ctggcgaacg attccagttc acactggaac gggtaggcag agaaaaattc
     5581 gaagagaaga tctgggaggc ttacaatggc tgaatttcat attgacgaca cagtagatat
     5641 cacagatgtg gtttgcccgg tgacatttgt caaggcaaag gtggcactgg aagaactgga
     5701 cgacggacag atcctttcca ttcacatgaa tgacggcgaa ccggtacaga acgtgccgag
     5761 aagcatcaag gaagagggtc acaagattct gaaactctta cagaatgagg atggaacgta
     5821 tgatctgatc gtcaagaaag tgggtgattg aatggcagaa agagtaaacc aggagacatt
     5881 ccagaaagaa gtgctggagg cagatggtct ggttctggcg gatttctact cggacagctg
     5941 cgttccctgt aagcggctcg caccggttct ggcggaactg gaagaaactt acggcgatgc
     6001 gctgaaagtg gtaaaggtca atatcaactt tgatctgccg ctggcagaaa aatgcgaagt
     6061 gaccgcagcg ccgacgctga tcttcttcaa aaacggagca gagcaggaca gacgcagagg
     6121 tctggtaaaa aaagcagaat taacggaaat tgtagagaat ctgaaataag gaaacggttc
     6181 tcatagaaaa gcatttcgtt ctatgagaat cctttccggt acataaagga gtatatcatt
     6241 atgaaaatca cagttgcagg tgtacagaag gaagtcgcag acggtctgac cgttgcacag
     6301 cttatcgtag atgagaatgt ggaaacaccg cagtacgtta ccgttaccat caacgacgag
     6361 ttcgtagaga acggtacatt tgaaaagact gtcctcaagg acggcgacaa tgtagagttc
     6421 ctctatttca tgggaggtgg acagtaagat ggcatttacc aacgaacaga tcgagcgcta
     6481 ttcccgccac atcattctga aagaagttgg cgtaaaggga cagaagaagc tgctgaatgc
     6541 caaggtgctg attattggtg caggcggtct gggcgctccg gcagcaatgt atctggcagc
     6601 agccggcgtg ggtactatcg gcattgcaga cgcagatgag gtagacctgt ccaaccttca
     6661 gcggcagatc attcatgcta ccgctgatgt gggcaaggcg aaggtacagt ctgcaaagga
     6721 aactatgggg gcgatc
//
LOCUS       DAWENO010000113         6675 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4617_length_6675_cov_8.351662, whole genome shotgun sequence.
ACCESSION   DAWENO010000113 DAWENO010000000
VERSION     DAWENO010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4617_length_6675_cov_8.351662"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2318..2327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3264..3273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1374 a         1766 c         1723 g         1792 t
ORIGIN      
        1 tgtcagagtc ggaagtgcat ttactcgaat tgtattattt tccatcatgc tcacctcacc
       61 caatgctgcc tttcatttcc agacgaatca gattgttcat ttcgatcgca tattccagcg
      121 gcagttcctt ggaaacattg tctgcgaaac cgctgacgat gcaggctctg gcatcctctt
      181 cgctgagccc tctggacatg agatagaata tcgcctcgtt gctgatcttg ccgatctgtg
      241 cctcgtgtcc cacgtctgcc ttgcgggtgc ggacatcaat tgccggaatg gtatccgact
      301 gagactccgc atccagcatc agtgactcac aggataccga ggactttgca ccctctgcgt
      361 tcttgttgac cacaacggag ctgcggaatg tgctgatgcc gccgtctttg gagatggact
      421 tggtgttgat atacgaagtg gtatccggcg cagcgtgtac tacctttgca ccggtatcca
      481 ggttctgtcc ctttccggca aaggtgatgc cggtaaactc catctttgcg cccttgccct
      541 tgaggatgct catgggatac agataggaca catgagagcc gaacgagccg gagacccatt
      601 ctaccttgcc gccctcttcc acctgcgcac gcttggtgtt cagattgtac atattcttcg
      661 accagttctc gatggtggaa tagcgcaggg ttgcgttttt gccgacgaac agctctacac
      721 agccggcgtg aaggtttgcc acgttatact ttggtgcgga acagccctcg ataaagtgga
      781 gatatgcacc ctcatctacg ataatcaaag tatgctcaaa ctgacctgcg cccggtgcat
      841 tcagccggaa ataggactgc agcggaattt ccacggacac gcccttcggc acatacacaa
      901 acgaaccgcc ggaccacact gcgccgtgga gcgctgcgaa cttatggtca gtaggcggaa
      961 ccagtttcat gaaatgcttc cggatcatct ccgcatattc gccgtgcatt gcactttcca
     1021 ggtcggtata cacaacgccc aggtctgcga cttcctggcg tacattgtga tacaccagtt
     1081 cggagtcata ctgtgcgccg acgcctgcca gagactttcg ctccgcctgc ggaataccca
     1141 ggcgctcgaa tgtattcttg atatcctccg gcacatcaga ccatttcgcc ttcatcttgg
     1201 tattcgggcg aacgtaggta acgatgttgt ccatgttcag cccgtcgatg gacggacccc
     1261 agtccggcac ttccagttcg ttgtagatct ggagcgagtg cagacgaaac tgctgcatcc
     1321 ataccggatc gttcttctct ttggagatct tttcgaccag cgccggcgtc agaccggcat
     1381 ccaccttata gaaatcggtt tctttatctt taatatcata gacacttctg tcaatgtctg
     1441 cgatctgctg tttttctttc acgtttttca ccctttcggt catcctgcgg caattattta
     1501 ctcagcgttt tcgtaagctg cgaaaccatt ctcgttgact tcttcgatca gcgagctgtc
     1561 cgaagtcttg acgatctttc cgtttaccat gatgtgtgta taatccaccg gcagagcctc
     1621 caggatcttg gtgctgtggg tgatgaccag cagactgccc ttggactgtt tccggtatgc
     1681 ctcgatgccc ttggaaacgg tgcggactgc gtctacgtcc aggccggagt ctgtctcgtc
     1741 cagaataacg aattccggat tcagcatcag catctggaga atctcggtct ttttcttttc
     1801 gccgccggaa aagcctacgt tcagatcacg ttctgcatag gacggatcca tctggagcag
     1861 ttccatcttc tcttccagct ctttcttaaa gctgaacaga cgaatgcgct tgccggtctt
     1921 tgcctccaga gcgctgcgga taaaggcgct gacggtgatg cccggaatct cgatggggtt
     1981 ctggaacgac aggaaaatgc cgttcttggc acgctcgttg tccggcatct cgttgatgtt
     2041 ctgtcccttg taaataatgt cgccgccggt tacggtataa gccggattgc ccatgagcgc
     2101 cgcgcccagt gtagacttac cggtgccgtt cggtcccatc agtacatgta cctcattctc
     2161 gtggatatcc aggtcaatgc cgtggaggat ctctttttct ccggcacaaa ctttcagatt
     2221 ctgaatacgc agcagatcgt tatgactcat gttacatctt ctccttcttc atctttataa
     2281 tttaccgcat cggcaagggt aatggaattc agatagtnnn nnnnnnnctt tccagatcgg
     2341 aagggtgtgg caggaatctg ccgtttcgca gatcggcgtg ccgttttcga tgcactttac
     2401 aggttcgatg gactcttcca ccgcacgcag gatttctccc acgctgtatt cttccggcgg
     2461 acgggacaac cgataaccgc cgccttttcc gctggcagcc tctacaaatt tctgcttgga
     2521 cagcatagat gtgatgcttt ccagatattt gagagaaatg ttctgctggg cagaaatgac
     2581 tttcagcggc gtgtaagaat ggtcgtccac ctgcgccagg cacatcatca gcttgagcgc
     2641 attgcgtcct tttgtggaca cgatcatagg ttaccacttt cctttgctcc gggaatttct
     2701 gagaacagtt tgacatattg ctttcagtat ttccctgttt gtccctatta tacacctacc
     2761 aacctagtat gtcaatggga cgaaatgcag gtttacaaat tcttcacgga gaattttcat
     2821 gatatgggat gtggatttct tagagtctgt tctgtatctt tttatgtgcg gattttcaag
     2881 catgattttg tcggtgacaa gaaaaaatcc gcaggcatac tatcgtatgg caaggatttt
     2941 tgacgcagtc agcggcgaaa tctgccgaaa agacgtgtgg aaaaagatac ggaacaggct
     3001 cttagggaat gtgacgacaa aaagcgcacc gttttgagaa cggtgcgctt ttgattatga
     3061 atcgtcattt ttgcagtttt ccgtttaccg ccggaacaat ccccgctttt tcggcttggg
     3121 ttcagcacag cgctctgcaa tgatctgcac catttcgcct acgtttttca tgccggaaac
     3181 ttcctgcatc ttgaacttca ttccgaattc cttttccaca gcgtcgatca gattgatatg
     3241 ctcaatgctg tcccagtctt ctannnnnnn nnnagtcttc tacgtcgttg gcggtagtcg
     3301 tctctgtgat taccagagaa tcatcgtcga aaatatcctg gaagatctcg ttcagtttct
     3361 ctaaaattgc ctcgtgtgtc atgccttctg atgctccttt tcatttactt ggatcacatg
     3421 gttctgattg ctgtacccgg caacatccaa atgccaggtg ctgtcgccgt tttcgaactg
     3481 ttccgttttg gtgaagccaa acttgccgaa cagatcccga accatgccgt tttttgccgt
     3541 ctggtagtag tagccgcaga tgctgcggat gtttcgctcc cggcacgccg ccaccagcgt
     3601 gtccagcatc gccagttcca tgtcccgttt caacacacgg cagctcatga gccaaagctc
     3661 aatgtgcagc gtgtctcccg agatttcacc gatcaccacg gaaacaatgc cattgtcacc
     3721 gaatttatcc cgcagccgtc cgtaaagtcg gatatgcttg tcgtctgcgg cgatctgttc
     3781 catctcgctc tgggtatagc gccgtgtggt cagattgaac tggttactct tattggtcag
     3841 ctggacgata cggggaagtg ccaccgggtc gaagtcccgg atctcggcgt gcatatccag
     3901 ccccagcaga tagtcggtgt agttggcgta agtctgctgc atctgcgcac gctttgcgtt
     3961 ggcacggtac atctcgctgc ggttggcatc cgccgccgaa aaagtcgtcg tttcaaaata
     4021 gccggcatga tccaggacac ggatgcagtc tgtggggctg tcaaatgccg gaacggcaat
     4081 gccggggatc tgtgcggaga caatatcccg ttctgccggg ttgtcatcca caaagacaaa
     4141 gctttccggc agcagcgaaa gggtttctgc ggtgtgcagc aggttctggt ctttcggctc
     4201 ccagttggca cagacggtga caaaatcctc ctcatgcagc acagagtccg gatgggacag
     4261 ccccagcttg gcgttctccg gctcattttt ggagcatacg gtcagcagca cgcccagctg
     4321 tttctgtgcc ttgcagtact cctgcacctc tgtgtatgcc tctgcctcgg cagtctcctg
     4381 tccgatggca atgccctctt gtccgtcatc gccgatgaca ccgccccaga gcgtgttgtc
     4441 cagatccagc gccaggactt ttttgttctt gccgtagata gagcggatga ttcgggtcag
     4501 actgtaggcg aattccggga cagcgtccag agtcatggca tacttgtaca gataccagta
     4561 ggaatcgtcg tgccaggcat ccagcccata gcttgccgcc agatagtgca ggtcatggag
     4621 ataaaagtcg gaatgggatg cggcatactg tccgaatttg cggttcagtt cctggataaa
     4681 ccagaccatg ccgtgacagt cccaggcatc ccggtttccc atgagccgag tggacggcag
     4741 ttcaaagttg ttctggataa tgggacagtg atagacttca tgcagacgct cccacatctg
     4801 gctgaagtgg ctgaactggc gctccagtgc atcccggacc tcctgctccg atgcagaaag
     4861 gggcagcggt gcctcgctga tattgcggct ggtggtgtga atgaagatca ggtctggctg
     4921 gaacgattcc agttccggat tgtcaaacat ggcatcctgc cagtactgtg catattccga
     4981 ctgataaaag gcaggctgaa tgccgcagtt cagcaggaac agttccagca tggagatgat
     5041 atcgtttgtt gttgagccgc ccagcacggc aatgcgcttt tccatgcgtt ggctgccgtc
     5101 tgccaggagg ctttttttca gtttgcgtcg ttttttcagc agaaagctgc tgtcaaaggg
     5161 atattccagt tctttcattg tgagacctct ttactgcgtc cggatggttt ccagacattc
     5221 gctgttgccg ccggtggcgc tgaatgtgtt catggcgaac aaaacatccg ttgcaccgac
     5281 ctgctgcata aaggaaacgg cattcaaatc gaaatagcgc atatcaatga catagatgtc
     5341 cgagaaggac tgcgtcagat acggcacaag cgcattgccg taactgtctt tcacgatgac
     5401 aagcgtccgc ttgttggtga catcggtctt gacgtgtgtg atcttttcgt cgccgcccat
     5461 gaataccaga taccagctga ccggttctac attgtccagg gagatcagca gcttgccgtc
     5521 ccgttcgttg gtgaaattgg tgtcgtaata ctgggtggtg tactggttgt gcggcgtata
     5581 ataggtgaaa tcttccgggt tctcctgcaa tacggcgctc ttggtaaagg tgtacatact
     5641 gcccagatag ccggaaagtg tggtcttggt gtagctggac aacggcgcaa agttcacgcc
     5701 ggctgtcttg gcaaacgcct ctgccgcata gtaagcgccc aggggcagcc agtggtggtc
     5761 ggtacgtgca tagatgtcct cgtcggtatg cgccgccagt gccgcatatg cgtccacagc
     5821 tttgacgttt tgcaggttct cgtcaatgtg gtcgatgttg tccgactcgc tgccggtata
     5881 accggaatag gagtccggca gataaaagga aactgcggta ggtgcgacca gggaatagat
     5941 gttcacctta tcgcccagct gtttcttgta ttcgttcagt gttgcggcgt atttttctcc
     6001 acgggaaaag ctgccgccgt agatgctgat ggcacgcttg tccaccagaa caatgccgtc
     6061 tccgatctca atggggtctg cctccggttc ggtggtgaca gtagtgaccg ttgtggtcac
     6121 accggcgctg tttccgctgg caaccggcgc agtggtggtg acattgtctt tcttctcact
     6181 ctccgtgtct ttcttgaccg gcgcaatgtt gccgtagaac tgcaccttgt cttcaccctg
     6241 caagccctgg tagctttcca gtttgcttat catatgcttg aagttgctgc gaccgggaac
     6301 ggtgtcctcg tagtagtgca tccagtctgc gatgtaatcg ccgctttcca gtgtgtccat
     6361 ggaaaaggac ggcggtgtgg caagcttgcg gttttcttcg gcggattctg tggggcgatt
     6421 gcccaccagc agaaagatgg agccgccgaa aatgatggaa ccaatggcaa agattcggac
     6481 aacagcgccc acccgattgg taatcaaaat ctgtcgtttc agtttatagc gagccttccg
     6541 ctctctggcg gattctttgt cctccggcgt atttgccgct ggtgcagttt ccgataacgc
     6601 ctcttcggca atctcaaact gcggcgcatc ttccgtcggt gcagaatccg cagcaggcgg
     6661 ttcttccgct ggttc
//
LOCUS       DAWENO010000114         6641 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4645_length_6641_cov_11.705284, whole genome shotgun sequence.
ACCESSION   DAWENO010000114 DAWENO010000000
VERSION     DAWENO010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_6641_cov_11.705284"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1229..1238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1640 a         1837 c         1549 g         1605 t
ORIGIN      
        1 agtcgtaatt atcgatttgc tttttcaact gtgcttttgt tcagcgtttc cttagaaaac
       61 atggtataat agaagctgta tttcaccgga tttaaggagg attttatgaa aaaggaaaca
      121 aaaacagcgc cccagacggc tgtgccggaa aaaccggcag caccggaacg ggtacacgga
      181 ctggacttga tctcgaaata tcgtggagcg atcatgggct ttgccgcctt gtggatttta
      241 tttttccacg agtgggtgac tctgttcgtc aacaaccagg taggcgtaaa cattgaggga
      301 tatctcaagc gtatcggctt ttgcggcgtg gatatctttc tcctgctctc cggcatcggt
      361 ctgacctttg ccatccgcaa gggcaatgtc ctcaccttct attatcgccg catcaagcgc
      421 attctgctgc cgtttctggt catggcgatc atccgatgcg ccctggagaa atggcccatc
      481 atcgaattct ggaaaaacat ctccggcatc aacttttaca ccaaaagcat ctacagcttt
      541 ctctggttcg ttccggcgat tctgacgctg tacctgttct tcccgtggta ctacaagctt
      601 ttcaccaaaa cgaaaaagcc ggtactcttt ttcctctgct ccctggaggt ctggctggta
      661 ttttccctgt ttgtccggga aaccatgcgt ggcgacctct atggtttcac aaaccgcatc
      721 ccggtctttc tggcaggcgt tctgctgggc tggctgacac agcagaaaaa gaccaccttt
      781 accaaaggca catggtgcct tatcgccatt acctttgtga caggacttta tctggcatac
      841 atcaccaact acaaagacta ttatctcctg gttcccacct cgaactgctg cgttccgaac
      901 tttatgatct ccgtttccct gccgttcctg atggcaaagt tcctggatat tctgagccat
      961 gccaagtaca ccaagccggt aggcatcggg ctgacaaagg ttctgtcctt tttcggcaca
     1021 ttcagcctgg aattttactg cgtccaggaa tggctgggct ggaagatcat gggcaagatg
     1081 ttcgaacagc agcgtccggg ctggctgatt aatctggcag ttcttctggc gatcacagcc
     1141 gtttcctttg ccgcacattt cctgttccag cagttctgga agctggtgga ctggctcatc
     1201 ggcatgattc ccaagcctgg caaaaaggnn nnnnnnnnaa aaggcagccg cagaacaatc
     1261 caaataaccc atccccacaa acacaaaacc ggtcatgcag acacacatct gcatgaccgg
     1321 tttctttttt caaggcatta ccgccgcacc agcacaccca cggcgctgca tcccaggaat
     1381 accaccaggt cggcaatgac taccgttgcg cccaccggcg tggatgccag aatggagatc
     1441 agcagccctg ccacagcgca gaccaccgag atcaccccgg cacagcagat cacgccccga
     1501 aagctccgaa acagccgcat tgccgaaagc gccggaaaaa cgataagcgc cgatgtcagc
     1561 agcgaaccca ccaggttcat ggcaagcacg atgatgactg cggtcacaac agcgatcagc
     1621 aggttgtaca ttctcgcacg gatgcctgtc gccgccgcaa aggtctcatc aaacgtcacc
     1681 gcaaatatct tatgataaaa cagcaggaaa attgccatga ccacaatgga cattcccacg
     1741 cagacccaca catctgttgt ggaaagggtc agaatcagcg tagagccgaa caatgtactg
     1801 caaacatctc ccgagatatt ggcggaagag gaaaacaagt tctgcaccag atagcccagc
     1861 gcaagggcgc tcacggacag catggcaatg gctgcatcgc ctttgatctt tgcgctctga
     1921 ttggcgcaca gcagtatcac agcacacgcc accgtcaccg gcatgaccag cagcatactg
     1981 ttgctgagcc gcagcactgt ggcaatggca agcgcaccaa acgccacgtg cgacaaaccg
     2041 tcaccaatga cggaaaaacg cttcagcacc agtgttacgc ccagcagaga cgaacacaga
     2101 gcgatcagca cgccggcaat cagcgcatac tgcacaaatg aaaacgacag atagcctgtc
     2161 agttcttccc agatactcat gcctgtacac ctcctgtgta tgcctgtccg atcctgctcc
     2221 ggagatagtc tgcctttgtg ccgaaaaaca gcggcgtctt gctgatgtgc agcacatggg
     2281 acgcatattt gacagccgcc tccatgtcgt gggaaaccat gaggatggtg atgccgtcct
     2341 cccggttcag ctttgcgatc agttcgtaaa gctccgtctg cgccttgggg tccagcccgg
     2401 tcacaggctc atccagcacc agcattttct gggtcgcacc cagcgcccgt gccagcagga
     2461 cacgctgctg ctgaccgccg gaaagttccc gatagcaccg tttcgccaga tgggtgatgc
     2521 ccagccgttc catgttgtcc agcgccagct gcttttcctg cctggtgtaa aacggccgca
     2581 gtccgctgcg gttcagacag cccgaccgga cgatctccag caccgatgcc ggaaagtcac
     2641 gctgtactgc agtctgctgt ggcagatagc cgatctcgtc gggtttcagt ccgtctcccg
     2701 tttcaatttt tcctgccatg ggagatttca gccccagaat ggctttcatc agcgtactct
     2761 tgccggaacc gttctctccc acaatataaa ggtaatctcc ggcgctgacg gaaaacgaca
     2821 gatgctccat cactgccgtt ccctcatagc ccagcgtcac atcgctgcat ctcaattgtg
     2881 ccatgtttcc ctgctccctt cgttcagcgc cttttccagc acgctgtagt tttcctgcat
     2941 cttcgagaga tatgttgccc cctggtcgat ctcgctctgc gacaccgcct gcagggactg
     3001 cagcgtcaga atgctgccgt ccgtgccagc gttttccaaa atcgtctttg ccagcgaatg
     3061 attgctgccc tccatggtca gcacacagga cagccccagc gatttcacct tgtccgacag
     3121 aaagacgatg gtctcaaagc ttgcctccgt ctctgcggaa cagccgggaa aggcggcgta
     3181 ataggtcagc ccgtaatcct ccgccagata gcggaacgga aagcggtctg ccaccacaat
     3241 ggtgctgcgc tctgccgttt cggtcatggt ctgatactcg gcgtccagcg cctgtaactg
     3301 cccggcatag gcggtataat tcgcctggta cgcctctgcg tggctggggt cagcctggca
     3361 cagggcatca cgcattgccg cacacgccag ctgcgcacgc cgcagagaca accagacgtg
     3421 ttcatccacc tctgtttcct cttctgcgtc atggtcatgg tcgtgatcgt ggtcatgatg
     3481 atggtggtca tcgtcctgca ttccctctgc caggacttcc tcgtcggcgt gcgccgcatc
     3541 caccatagag accagctttg ccgtatgatt ctccgtggtg tccagcacat cattgaccca
     3601 ggtgtccgaa acaccgccca cgtagcagaa cacatccgct ctggaaatgg cggcgatatc
     3661 cttaactgac ggctggtagc tgtgcaggtc tgtcccattc ttcaccaaca gggtcaaatc
     3721 tacactgtcc acgccctggg tcagctgtct cgcccagtca tattccggaa agatggtgca
     3781 gaccacctgc aatttgccgt ccggtttcgg cagtgtgccg ccgcagccgc tgaaaaaaac
     3841 gctccatccg aaacagagcg tcagtaaaaa tgccagtttt cgtttcatct tgttctcact
     3901 tccatattgc ctgtgcagtt ccacagtctg cctgccatac gttctgcatc tctgtttgaa
     3961 attgaaaatg attttcatat tcagattata gcacatattg aagcacttgt caagcccgtt
     4021 ttgcagaaaa agaattccaa ttgaaagaca gacctttttt tttgcagttc tgacctcata
     4081 ctatcataag ttccgttgcc tgtgcttatt ccaccgcaca cgggaattaa aaaggaaatc
     4141 gtgctgccat aagaatttta aggaaacact tgacgaaaag agagaaatgt agtaaaataa
     4201 aaatgatatg actcggaaag gagcagtgaa aaatgctgac agagaaaatg acctacacgg
     4261 tttctaccat caccccgaac acacaggttg gtgacatcgt ctatgtaaag tcgctggatt
     4321 ccggtgcgca cgccatcggt gaagtcgatc acgtttatgc acacggcatt cacgtcgatc
     4381 tctatatggc aaagcccatt gatgagacaa cttttgaggt aacccgaaag ctgtacccgg
     4441 aatttgtcaa tattcataca tccaccaaca acagcacaac acctgcaaac gcataagagc
     4501 gtctgcggac aagcgaaaag ctgccggcaa ttttttgccg acagcttttt tcatgcgttc
     4561 ttttgttttt gccgtttcca gacctgctcc agcagcattc cgatcagaaa cgctgcgata
     4621 tcgccaaagg tggaaatgat ccgcagggtg acggcgtaca gcataatggt attcatatcc
     4681 agaaagctgc cgcacatcat tatcataacc gcctcacgga taccgatgcc gccgggcgca
     4741 cccggtgtga taaagcccac gatccaggag aacaggtatg ttcccaggaa cagtcccagt
     4801 ttatccccag gcacatcgaa caggaatgct gccggaatgg catacattgc cgtggagatg
     4861 atgtactgca ccacgtacag caggaacacg cccagcacac gcagcaggat cttccggttc
     4921 agcagtttcc ggtacttttc aaagtagcgc tgcagcggct cccggaactt ccagcgcagc
     4981 accaggaaca acagcagcag caccgcaacg ccgacaccca gcacaatgag gaaattgttc
     5041 cggtatgtgc ggatcagttc cagcatattc tttcccataa gcaccacgat cagcagcagc
     5101 ggcgtcagca tggagcagac gatatccagc acagtggcac aggctacgtc cacgtggctg
     5161 atatgaaggt cggcggcaag ctggtttctg cccacatact gaaaaacatt gcccggcaca
     5221 tatttcatca gattcgactt ggtatacacc ggcagtgcat ctttcatggg aatatttgtc
     5281 ccggacagga tgcggacgaa ccgcacccag ggcatggaca taaacagaat cacagccgtc
     5341 tgtatcagca cacagccggt aatcattgca atcagcctgc ccgacgcaaa catcgaccaa
     5401 tctacatcca tggtcacgat cttctttacc acaaacacca gggcgatcac cgtcacaatc
     5461 gtcccgatca atttatagta ctttttcaaa aagcgcatag agaatccttt ctgttgtaaa
     5521 ttttaggcga caccgcacaa agattgtgcg taagatgcgc agccggattt ttcggcagat
     5581 tgcatagaaa ttccgcagcc atactgtcgt atggcaaggg atttctgtgt gatatgacgg
     5641 aaaatcagca agcagattgc gtgcaaagct gccaagcgtg ctttgtgcgg tgttgcctta
     5701 taatacagtt tattgtacca tataatacag tctattgtac catgttttcc ggggattgtc
     5761 aattgtggga aaagagaaac agccgctctg cattttgcag gacggctgtt cattctgttt
     5821 gatcttcttc gcagtattcc agaatatctc cgatctcaca gtgcagcgcc gcacaaattc
     5881 gtgacagcac ctcactgtca tatcgtgtga cacgattctt gtagtaatta tcgatcaccg
     5941 gatactttgt tcccgtcatc tgcgcaagac gataccggct gatgccacgc tcttgcagca
     6001 gctgttccaa ccgcagccga accatcatgc cacctccaaa gaaaaaacat ctctttttac
     6061 tatacaggat attttttctc ttgacaattc ctttatacaa gtatataatt atataaggat
     6121 atagtatgga ggtttgcaat gatagactat gaaaatttat attatgacac acagaaacaa
     6181 gttacaagaa ccatacaaat tttagaacac gaattagaac atctgaaagc ctttcaatgc
     6241 ctttgtgaaa atcgggttct tgcacctgaa tttgaggtta cacaccaaaa caagccacgg
     6301 gaaaattaac agagaaacag ccgctctgca tcataatgtg caggacggct gtttggattc
     6361 cagtgtgaaa tttttatctc ggcttgtgcg cacgaacggt ttcaagcgtt gcgttgggat
     6421 tgctcttttt gaagattccg aaggcatcct cgtcgctgat tcccggattc tcctggtaaa
     6481 tcttcttgat tttcttgtcg tctccaggcg ttgtcgtttt gatgttattg tcactgcgac
     6541 gggcaattac atttaattcg tcattggtat agttgttttc cggtctgtgt gcacgaacag
     6601 tctcgagtgt tgcattggga ttgctctttt tgaaaatctc g
//
LOCUS       DAWENO010000115         6610 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4669_length_6610_cov_9.837223, whole genome shotgun sequence.
ACCESSION   DAWENO010000115 DAWENO010000000
VERSION     DAWENO010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4669_length_6610_cov_9.837223"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3762..3771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1733 a         1535 c         1666 g         1666 t
ORIGIN      
        1 tttttcttgg aaagggtaat gatgccggct gccaggctga cactaatcag cagatacagt
       61 cctttcggca gcattcccag cagtccggca gaggtgccta ccacggcatc atacatggaa
      121 ctgtcccgga acagtagcgc ttccacaaac agcagaatgc ccagcggtac gatgcagaaa
      181 ctggtcagac gggtgacttt ctgcatggag ccgaccagtt cagagtgcat ggcttttgtc
      241 ttttttgcct ccttggtcag cttggaggca tagctgtcac tgcccacacg gaggacagtg
      301 gcacggcagg tgccgctgat aacagaactg ccggacagca gggaatcgcc tgcctttttg
      361 tagactgcat ccgattcgcc ggtgagcagg gactcgttga cctcgatgcc gccgtctgtg
      421 acaacggcat cggcgcagat ctggttgccg ctttccagga gcagaatatc ctgttcctgc
      481 acttctgcaa tgggaacaga gcgcagttcg ccgttccgtt cgattttggc attcgactgg
      541 gacaaaagcg tcagcttttc gatcagccgc ttggctttca gttcctgtgc aataccgatg
      601 atggtgttca atgcgaccac cggaacaaaa aataagttcg accatgcgtg aacaaagatc
      661 agcgcaatgg caattagcag attcagcaga ttgaacaggg tacagatgtt gtcccgtaag
      721 atctgtcccg ttgtttttgc agcagtttcg ctggctttgc gtttcatatg cagacttcct
      781 ttccatttgc gggtgtgtat attttctcta ctcttagtgt atccggtttg ctgtaagagc
      841 gcataaacca aagttaaaca gaagataaaa tcggacatct gcacagagaa acagaaaagt
      901 catcccccgt accagaggat gacttttctg tgtttatgaa gaattaaaat gaagatatca
      961 aataggggag tttattttgt tgccggcagt gtcttgatga tctgcaccag gaattgcagc
     1021 aggacaatgg catcctcgcc gtttacttcc ttgtcagagt tgcagtcggc attggcagtt
     1081 gcctgtgcat tcagatctac ctgacccgct gccttcttgt tcagcagaac agcgtctgta
     1141 atatctacct tgctgtccag gttgatgtca ccgaaaatag atgccagaac agcagattcg
     1201 gttgtggtct cactgggctt tgcagaagat gcagtggttg cagtagcaga agtgcttgtt
     1261 tcagaaggct gtgatttttc agtcgtagca gtagcactgc tggtttcagc agttgtggta
     1321 gtttcctggg gaactgtggt agtctcgctg gaagatacgg tagtctgtgt gctgctttcg
     1381 gttgtgccgg gctttgattc agaagttgtc acaccgctgt ccggcagacc ggagatggtg
     1441 aactttacag atacagactt ggaaaccttg accttcgatg cgatatccga aaccttgttg
     1501 gtaggtgtcc atgtgttcag aatgttgtag cgcaggcttg tgccgctgtc gcccagacga
     1561 tatgcaccgt cagaaggacc ggtgtaggag atcggctttc cgtctaccat aacgctttcg
     1621 atcttcatct ggatgccgga atctgcgatc ggatctgcgg aatccttgga agcgtacttg
     1681 aaaatgtcaa tgtcaaagtc cagaaccagg caggagatcg tgtccgcctc gctggaaacc
     1741 tggaagcttg tagtgtacgt gccgtttccg gtaatgtttg ccgcagtctg acccttgttg
     1801 ccgttgcccc agaagtcatc tacgccgatc gaaccagcca tccagacctg gaacagtgtg
     1861 tcgccggcag gcggctgtac atcggtattt gtggttgtag ttgtggtttc gttggtgtcc
     1921 tgtgttccgg gtacagttgt tcccggtgta acatctccgc cgttattcca gctgcccttg
     1981 cccatgttgt tatccagagg caggctcatg ggtctgacgt tgataactgc gatacattcc
     2041 ttggcaacag ctgccttcag atcagaaact tcgctctcgg acttgccggc cgtgacggag
     2101 gaatccggtc tgtcactcca gtccagattc aaagaccacc aaagctggat ctcagcggat
     2161 gtgcttgcct gacagagctt agagagcatt ttcagttctg cggtatccgt aacttttcgg
     2221 ttttccgacc agcagtttac gttcatttga gaagtatcag tcagatcgac agtgcaggga
     2281 accggtacgt ttttattctg tgcggaatcc cactccacac cggcacggaa cggataaccg
     2341 atccaatagc ctttgccgtc tacgttcaga ttaaagcaca gcgcaccgcc gccgcacagg
     2401 gacttggtca tatcaatgtc gaaataaatt tcagcctttt cgccgatctc gcaggggaat
     2461 atcaccttgc cgtccggata cagggtgcag ttgctgttga caaccacgcc ctctataccg
     2521 ccgcctccga aatcggttct cggcgcaccg tattttttgg ctgccgctgt gatagagtca
     2581 tagatgaaag tcttgttggt tctgtccagg aaacgatatg cctcgttgtc gccctcagca
     2641 ttggagttat cccacagaca gcagctgatg cctcttgctc ttgcagcggc atagtaataa
     2701 gcggtctgat cggttcttgc ctgcgtgttg ttcaggtttg tcgcaccgaa ttcgccgatg
     2761 actaccggaa tattctgctg tacaaatgcc acatacaggg tgtcgaacat atcgtccagg
     2821 gatttcttca tgctgtctgt ttccggaacg ctcttgtttt ccagtgacag ttccggaacg
     2881 gtgtagctcc cttctgcgga aaccgtggta gctgctgtga tgggaagtgc tgcaatcaat
     2941 gtagatattg caagcatccc tgcaatcgtt tttttgatca taccaaatac ctcctgtgtt
     3001 gtttcgtgat gatttctgtc atcatttcac acgattcgtt tttcggtgtc tttagcatac
     3061 cacgttttcc aaagggaaac aagcaattca aatttgagtt ttccccctgt ccgtagagtt
     3121 ggcacaacga ttcgttgaac tgtagaatat ccacaaaaaa ataaaggaac ggtatagaaa
     3181 ttcaaccaaa aaaacagccc gtatctccag acgccgctgc ggcgcagaaa atacaggctt
     3241 tggttttctt tacgattcgc cctcttcgcc gtccggatag ctgctgtttt cccacttgga
     3301 caccgcctgg ggcgagacgt tcagccgcat ggcaagctgt tcctgtgtca gccccagcgc
     3361 ctttctgcgc ttggcgagat tgcgcccgaa taccgtattt cccattgtgt acacctcatt
     3421 tcagttcccg gcgctgtcat tttttgtcac agcgcatcgt atcttttcac acccattgta
     3481 gcatacccgg cacagaaaaa tcaataggcg cattccatct taatataggc tcccctaaca
     3541 ggggagctgg caagccgtag gcttgactga ggggtcgttc ctttgtgtca ccccaaacta
     3601 cccacactcc gcaaactaaa acaggaaaac aacatttctc cacacaaaag ttcacaaata
     3661 tttgctgtga tttccgtgaa aaacgcataa attttagcaa agagataaaa gtgaaagaaa
     3721 tatgcttgta atctgcgtca aaaaatgcta taataaatgt annnnnnnnn natagttgac
     3781 atgaaaacga aaacccattt ttaattttcc caaacaagga ggaattttat gcaaacaaaa
     3841 gtgaaacttt ccaaacgggt ggtgagcctg gcgacggctc tggtgacggg gctgtctgcc
     3901 gtgggtgtaa caggtatacc ccgggggggt atttggaacg ctatctgcaa gtgcagagga
     3961 cggtcacgat tacgtcaacg gcttttgtac tgacgagggc tgcacttcgc cgtatgaaca
     4021 gccggagaca gacgacaacg gtacatacca gattgcaaac gccggaaacc tctactggtt
     4081 cgcagagcag gtaaacgatg gaaacgctaa tctgaatgct gccctcacca cagacattgt
     4141 ggtgaacgag ggcaaccttg caaattatga cggaacaagt gaaaatactt ggagagaatg
     4201 gacgccgatt gcagctgatt acggaagata caaaggtaca tttgacggac agggacacac
     4261 aatttccgga ttgtacttta acgacagcag tgcagaatgt attggtatcg ttggagaact
     4321 gggcaggggt ggtgtaatcc agaatgttgg tctggaaaac tcgtactttc atgtatatca
     4381 gtacactgcc ggtatttgtg gctataatag tggaactatc agcaattgct acaacgcagg
     4441 aactgttata ggggaattca tcattattgg cggtatcgtt ggtgcttttg aggttgggac
     4501 tgtcagcaat tgctataaca caggcaacat tacaggagaa tcggatgttg gcggtgtgtg
     4561 tggtaacaat atgatgcgtt ctaatactat tgaaaattgt tacaacacag gtaatatcac
     4621 aggtgatatg gacctcggtg gtatctgtgg agaaaatact gcaaatgcta tcattaaaaa
     4681 ttgctacaat ataggaaatg ttacaggaac agataagaga attggcggca tcatcggtag
     4741 aaatgctagt aaattcgttg aaaactgcta caatacagga acagttacaa gcacaggtga
     4801 aaatacagat acagttggcg gtgtgtgtgg tctgaattca tccaattatc ttacaaactg
     4861 ctactacctt gccgacagcg aaactgacaa attagacggc acgactttca aaaccgccgc
     4921 cgaatttgca agcggtaacg tagcctacct gctgaacggc agcacaccgg gcaacgcaaa
     4981 cattttccgc cagaacctgg acaacggcga gccgaccgat gcgaccccgg ttctggacag
     5041 cgcccacgat gtggtttacg aaggaacgct ttgcacaagt gggacaggct acaccaacga
     5101 tgaagattcc acaagtgaaa ttcacgcctg ggacgacaac ggtttctgca aaaatgacga
     5161 cacccactac cagccggcga caaataacaa cggcacatac gagattgcaa acgccggtca
     5221 gctgtattgg tttgcggata aggtgaacaa tggcacacag atagttgatg ctatcctcac
     5281 aaaggatatt gtggtaaaca agggagacgt tgcaggctgt gaaggcacaa agacaaaggg
     5341 ttggagagaa tggacaccga tttcctatta ctttgataaa agcatgggcg gttttgatgg
     5401 aaagggacac accatcagcg gactgtattg caatgatgaa aataaagatg ctggtttatt
     5461 tgaaagggca ggatacatta gaatttccaa tgtcggcgtt ataaattcct atatcaaagg
     5521 aaaagaaaat gctggcggta tcactgccaa aaatgactca tatggttcta ttagaaattg
     5581 ctatagcatt tgtacagtat ctggttatga tgacaatgcc aaagtcggcg gtatctgtgg
     5641 ctccagttct tataaaatag aaaattgcta cagcgtaagt aacggcggtg tttgcagtgc
     5701 taccagtgct accattggca attgctacta cctctccgac agcgaaaccg atgatatcga
     5761 cggcacaact gcaaaaactg ccgagcagtt caaaagcggc gaagtctgct acctgctgaa
     5821 cggcggcaaa tcggatgaaa ccattgcatt ctaccagacc atcggcgagg acgactaccc
     5881 ggttctggac agcacccgca agacggtcta tgctgacaca gactgcgatg gttctgcgct
     5941 gaactacacc aacggctcgg atgtttccca atccgatcac gactgggacg acaacggttt
     6001 ctgcaaaaac tacccgaccc actaccagaa accggaagag gtcaacggcg tgtatcagat
     6061 cgccaacgcc ggtcagctgt actggtttgc ggcatatgta aacacatatg attatgataa
     6121 tgcaacatat ccgaacgtag ccgcaaacgc cgttctcacc gcagacatca cagtaaacga
     6181 gggtgatgtt gcaggctatg acggcacaaa ggcagagggc tggagagtct ggacgcccat
     6241 tgcgcctaat agagcaacgt atgccggcaa attcgacgga cagggtcaca ctatcagcgg
     6301 actgtatttt aacaatgagg acatagatga tggaaaatac gtcggtctgt tcggtcatat
     6361 ggaaaacggc ggcgaaattc agaatgtcgg cttaaaaaat tcttacttta gaggtcgcaa
     6421 ttacatcggc ggcatctgcg gttccaatta taaaaccggc acaattcgca attgttatag
     6481 cgaagcaaat gtgactggtg tagttgttgg cgctgatatc ggcggcgtag ttggcgagaa
     6541 taacggcaca gttgaaaact gctataacac aggaaatgtt gtaggtaata aatgggtcgg
     6601 cggtgtatgc
//
LOCUS       DAWENO010000116         6403 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4829_length_6403_cov_7.869093, whole genome shotgun sequence.
ACCESSION   DAWENO010000116 DAWENO010000000
VERSION     DAWENO010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4829_length_6403_cov_7.869093"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1547..1556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1421 a         1635 c         1836 g         1501 t
ORIGIN      
        1 ggaactggaa tttgtgaacg gaaagaacga gataactaag gcggcggcag aactgttcag
       61 cggaaactgc agtgtgatcc tgtactctat gggaaaagac ggggcaatgc tgctgacgcc
      121 aaagggcagc tgtaccgttc cgaatccgga tgtgcaggcg aaagacacca ccggtgcagg
      181 cgatgccatc atcggggcgc tgctcttctg cctggcagag caggatatca ccaaggaaaa
      241 tgtaaacgaa gtgactatgg aacagtggca ggcatggctg gaatttgccg tgtattatgc
      301 caactacagc gttatgggca gcggtgcgat cacatcgtat cccgaccgtg aaacatttat
      361 ccgctggaaa cagcagatgc agcagaaagg atgagaaatc atgggaatgt ttgatttttt
      421 gaagaagaat aagacggaaa ccacactgcc ggaggcgccg gcgaacacat tgctggcagt
      481 gcaaaacggc acgatcgttc cggtggagtc cctgcccgat ccggtgtttg ccgaaaaggt
      541 gctgggtgac ggctacgcca ttgaccccac agacggcaag gtgttttccc cggtttccgg
      601 tacaattatc gatatccagg attcgctcca cgcttacggc atctccacag atgacggact
      661 ggaagtgctg gtgcatatcg gtattaatac cgtttcgctg aacggaaccg ggtttcagac
      721 aaagcacaag gtcggcgaca agattcacgc cggcgaactg ctggcaacag taaacctgaa
      781 actgttagag gaaaaagaac tgccgaagtt tacggtagtt ctcattacag atatggatgc
      841 cgtcaaggca atccatcgca gcgccggaac tgctgaggca ggaaagacag cggcgctcac
      901 ctacgagaaa taagacaact ttcacccttc ccattcccgg tcctgcccaa acaggacctg
      961 cacaaaaaac cggaacgtat tttgctacgt tccggttttt tgtttctcac ggcagacact
     1021 cagagaatga aacagatcag accgctgcat ccctcgttga tgatgcgctg caaggtctcc
     1081 tggagtttca gtcgggcgtc cataggcatt ttggaaagct tgttgtgcag tccttcgccc
     1141 accagttcgt ggagcgactt tccgaaaatg ttggaggacc agatcttggt ggggtcttct
     1201 tcaaagccct ccagcaggta catgacaagc tcttcgcttt gccgttctgt accaacgatg
     1261 gggctgacag tggtgcggat atcggcacgc atcatgtgga tagatggtgc agatgcccgg
     1321 agacgtaccc cgtatctgcc gccctgtttg atgatctccg gctcttccag cgtcatttcc
     1381 tcctggtcgg gcattacgat gccatagccg gtagcctcca cttcccgcag tgccggttcg
     1441 attttggcat attctttctg aatgcggctc agttccagca ggataggcag cagatcgttt
     1501 tctccgtgga tctccagtcc ggtagtttcg cccaggatgc ggtaaannnn nnnnnnttcc
     1561 acttcggcgc agacgctgcc ggttcccatg ttgatctccc ggatgccggc acgtgtcaca
     1621 tagggacagg tgctgacagc atcggcaaac agggaagtat cccggatggt gcggatctcc
     1681 gatgccgcct ggcggatgca ggaaaagaca gattcccgca gccagtggtc tttttccagc
     1741 gtggcaatcc acttgggcat acaaacgttg atttcccgga ctggaaagac ccggagcagt
     1801 tctgtgagaa tgttgcagat gtccgtctcg tccaggtcag ggcagctgac cggcagcacc
     1861 ggggcgctgt acttttccga cagctgtttt gccattgcct ttgcctctgc ggtgtgcgga
     1921 tccatacagt tgagcaggac ggcaaagggc ttacccagct cctgcaattc tgtaatcaca
     1981 cgctcctcgc attcggcgta ttcggcacgg ggcagatctg tgatgctgcc gtctgtcgtg
     2041 acgactagac cgatggtgga atgctctgcg atgacctttc gtgttcccac ctctgccgcc
     2101 atattaaacg gaacttcctg gtcgaaccag ggcgtatgta ccattcttgg ctgttcattt
     2161 tcgatatagc ccagggcgct ggggatgatg tagcccacac agtcgatcat gcgaatccgc
     2221 attcgggcat tgtctcccag cgtcagttcc acagcatctt ccgggatgaa cttcggctct
     2281 gtggtcatga tcgttctgcc ggcggcggat tggggcagct catccgtggc acgttctttc
     2341 cacgcctcgt tctggatgtt ggggatgacc agctgttcca tgaaccgttt gatgaaggtg
     2401 gactttcccg aacgcaccgg accgaccacg cctacataaa tgtcgccgcc tgtacggcag
     2461 gcaatgtcct catagatcgt atttgccata gttgctgtac ctcctgccgt tactgcacaa
     2521 tggggatgaa gagcatccct gctgtggtca gctgttcgtt tgtcagatcg ttttcctcca
     2581 tgatcgcccc gacgctggtg cggcaccgtt tggcgatatc ccagacgttt tcatgctcta
     2641 cgccgtagta cagcttgagt gcatagtcgc cgtcccgtac cagcttgttt tcgccgtcca
     2701 ccgtcagccc tgaaaggcag acgtgccgtg cagacgggca gagacagcca cggagcatca
     2761 gcgttgcttg aacggagatc ctgccgtcgg aggacagatg ataggtacag tcggtgggtg
     2821 tgacctccgc ttgcaggacg gcgttgtcta ccggggtgtc cgaatcagtg tagacctcga
     2881 atgcctcttc cttttccagc agcatcggcg ttccctcggc atcgccggca aggagactgc
     2941 agcagagcat accgctgacc cggatgcggt tggtgtcgga gagcagctgc atattgatgt
     3001 tgcgtacctg gcagtgcaga tcgtggatgc aggcaggaat cgtgtcgcct ggctggagtg
     3061 ctgcgctgca cggcagagca gcacagatcg gctccggcgg catatcaatt tgcagggaga
     3121 cggtttcctg ctcacaggga tagacggtgg aaaatgcgtc tgtgaccagc tggatggatg
     3181 cggttttgac tgcggtgcag cagagacgca gttcgatttc acactgcacg ctgtccatgc
     3241 tgccgccttt tccggggcag ggcttgatgt cacagcggat gacctgtgcg tctacactgc
     3301 cacgataggt gtcgtcgatc tgcgccatgt ctacgatctg gctgtacggc acggtgaact
     3361 gcatctgttc catgccgttt gtaccgtcct cctgctgcca ggtgtagagg aactgcaccg
     3421 acgcctcgcc ctttgccacc agtttcccgg cgagaatgga atgctcctca tgtcccagcc
     3481 ggacgctgct gcggacaatg ctgcgaatgg gcggcttgga tgcaccgagt tccacatcct
     3541 cgttgagcag gatgtgcttg acggcacggc gtttttgtgc ggcgtattcc acagggactt
     3601 tccgcagctg tacgtgcatc ccgaaaacat cctggatgac ctcctgccga cggtcgccgg
     3661 tgacgtggac tgcaacagaa acagcgccac gcacttccag ccgccgtggg ctgactgccc
     3721 ggcagttggc atacgtcgtt ctgggacgca gggtgacgat ggggtcttct gacgggcgtg
     3781 gcagttcaac attgcgggtg aatgtcatct gctgtgtcac gcactggagc agggtactgc
     3841 cggaaccgca gtacagcacc cggatgtcgg cacgcagttc ataggtgatg cggtcagtgc
     3901 ctgtggtgga tgtgacgatg gtgggctgga tctcacagcc gatcatgcga aaaatatccg
     3961 gatcatagtc cggcaggaca tagtccagtt caacgctttg ttcctgcatc tcgtccagaa
     4021 tcacttctgt aacgggaagc atttcccgat tgattttcag atccatatgg aactcctcct
     4081 tttttgccgt ccagcggcgc tggtttaggc agcaccagtg gggttctgcc tttgcctaat
     4141 cctatgcaga caagcggctt agtagaacaa cttatggtgt gtttctgttt ataatgtcta
     4201 tcaaatccct atgaatcatc cgtattcctt gtgcaatcct acaaaaccgc cggattccta
     4261 ttgacaaatt actagttttg cagtatgata tgtaacatac taaacatatc aattcagtat
     4321 gaattatcgg atatcaagga attgaccgga tcagcgctga atgggaaaga gggaaaccat
     4381 atgaagatca aaaagtgcat cggtctgctg ctggcggcag ttttgacggg tgggatgctg
     4441 agcggctgcg gaaacagcaa taccgtcagc atcgtcaatg tttcctatga ccccaccaga
     4501 gagctgtata ccgaatataa tacgattttt gcagagcatt ggaaagaaca gaccggacag
     4561 gatgtggaag tgatacagtc ccacggcggt tcgggcaagc aggcgctgga agtggcaaac
     4621 ggtctggagg cagatgtggt gacactggcg ctggaatatg acatcgactc catcgaaaaa
     4681 gccggactca tcgaggacgg ctgggtggac aagttcccgg atgacagcgc accgtatacc
     4741 tcaaccattg tctttctggt gcgaaagggc aacccgaaaa acatccggga ctgggatgac
     4801 cttgttcggg atgatgtgga tgttatcaca ccgaacccca aaacatccgg cggcgcaaga
     4861 tggaactacc tggctgcctg ggcgtatgcc gaccggaaat acggcggcga tgaacagcag
     4921 acggaggcat ttatcaaaaa gctctaccag aatgtactgg tgctggattc cggcgcacgt
     4981 ggcgcaacga caacttttgt cgaaaacgga cagggtgatg tgctggtggc gtgggagaat
     5041 gaggcgtatc tctccatgcg ggatgacccg gatgaatatg agattgtgaa ccccagcatc
     5101 agtatcctgg cacagccgtc tgtggcagta gtggacgatg tggtggaata tcgtggcaca
     5161 cgggacgtgg ctaccgagta tttgcagtat ctctactcag atgatgccca gcgcatcgcc
     5221 ggggataact attatcgttc gaccaatccg gagatattga aagaatatgc agatgttttt
     5281 gatctggaca ttgatctggt cagcatccag gatttcggcg gctgggatgc ggcgcaggaa
     5341 aaacattttt ccgacggcgg catcttcgat cagatctatg aaaaataagg agcaggtatg
     5401 agaaagaaaa gcaaacgggt gattcccgga tttggattgt ccctgggcgt gacgatctcc
     5461 atattaagta ttgtcgtgct gattccgctg gcatcgctgg tgatatatac ggcaaagctc
     5521 agcccggctg agttctggga gactatcaca agaccacgtg tgctggcaag ctttcgggtc
     5581 agcctgctga ccgcatttgt ggcatctgcc atcaatgcag ttatgggcgt gatattggcg
     5641 tgggtgctgg tgcggtaccg ctttccgttg aaacagctgc tgaacggcat gatcgagctg
     5701 ccctttgcac tgcccacggc ggttgccggc attgcgctga catcgcttac cgcagataca
     5761 ggccttgtcg gaaaatggtt cgcacgcttt ggcatttcca tcgcatacac cagagcaggc
     5821 attaccgtag cgctgatctt tgtgggaatt ccctttgtgg tgcgtgccgt gcagccggtt
     5881 ctggaaaagc tggatccgac ttatgaagag gctgccggtg ttatgggtgc cagccgggta
     5941 cggatcttct ggaaggtgat atttccggaa atcctgccgt cggcgctgac tggtttcgga
     6001 ctggcgttcg gcagatgtct cggagagtat ggcagcgtgg tattcattgc cggcaatcag
     6061 ccatacgaaa cggagatcac accgcttatc attatgtcaa aattgcagga atacgactac
     6121 accagtgcca catccattgc gcttgttatg ctagcggcgg cgttcctgat cttgttcctc
     6181 accaatctgg tgcaggcgag aagtgcaaag attctgaaag gaggtcagtg agatgcacga
     6241 agaaaccgtt ggttccaaaa tcgtcaaatg ggtgctgatc ggcgtcagtg tcttgtttct
     6301 ggtgctgatg ctgcttttgc cgctgctgac cgtaatttca gaggcactcc gggacggctg
     6361 gaaaacctat caggcagctg tcaccgataa atatacggta aag
//
LOCUS       DAWENO010000117         6279 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4928_length_6279_cov_11.842384, whole genome shotgun sequence.
ACCESSION   DAWENO010000117 DAWENO010000000
VERSION     DAWENO010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_6279_cov_11.842384"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6137..6236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1516 a         1477 c         1692 g         1494 t
ORIGIN      
        1 cgacctgttc tatcagatgt actacatcgc tctgcagggt gctggttaca aggatactac
       61 ttggactgac gtttgccctg agctgaagga actgaagggt tcctgctggt acaccggcaa
      121 gtaagttctg cttacaaaga acgtgaaaac gtttttgaca actcggatat gacatcataa
      181 gaaaaacccg atgcgggagc gatcccgtgt cgggtttttt gcgttctgcc gcaggaaacg
      241 gctcgaaaaa aggtttgtct tgacaaaccg ccggtcagtg tgctatactg agtggcaatc
      301 gcataaaata caacatacaa gagagcggtt ccgtgaccgg tggcgaatgc cggcggtgcg
      361 gcgacggaaa ggaagacgga ttatgggatg gatagaactg ctgctgacgg cggtcgggct
      421 ttcgatggat gcttttgcgg tgtccgtctg taaggggctt ggaatgcgca aaatgcggta
      481 cgaccgggca cttgtgatcg ggttgtactt cggcgtgttt caggcgatca tgcccctgct
      541 gggctggctg ctgggggtct cgttttcccg gtacatccag gcatttgacc actggatcgc
      601 atttatcctg ctggcgttta tcggcggaaa aatgctgtgg gatgtgttcc acgaaaaaga
      661 ggaggagacc acggaaaagg tggatgcccg gctggatcac aaggaattgt tcatgctggc
      721 gatcgctacc tccattgacg cccttgcggt gggcatcgct tttgcctgtc tggatgtgca
      781 gatctggagt tccatttcca tcatcggctg cgtgaccctg gtgctttctt tctgcggcgt
      841 atggatcggc aatcgtttcg gcaatcgctt tgagaaaaag gcagagattg ccggcggtct
      901 ggtgctgatc ctcattggcg tcaaaatcct ggtggagcat ctggtgcagc accaataata
      961 tgccaaaatt tcccttattc ttttcaaatt catgtttata tttttgtacg aatttgttac
     1021 aatatatgta tgattttgtg caaggaatga ggactttaca tggcacagga aaaaccagaa
     1081 catcatagaa agcgcagaca gcttgtctgg atgatcgtca gcctgctttt ggcggcggcg
     1141 gtgatcgttt cggcgatatg gctgcgttcc aaccgatatt tttattccag cgagtcgcac
     1201 tccctggagg cttttcgtgg gttagaagga aagggcgagg tgcagtcgcc ggaggattca
     1261 gcgaacctgg cacggcggca ggaactgctg ctgaacggtc aggcggagaa cgatgccatg
     1321 atcggctggc tggtgctgcc gggaacgatg attgattatc cggtcatggg cggcaaggac
     1381 agcagccgct atgcagaaca gaactatgac ggaaagtatg ataaatacgg aacgccttac
     1441 ctggcggaga cgtgctgcac ggatttcagc gattattttt ccgtgatcta tgggcatgat
     1501 atgaacaaca gcaccatgtt cggcagtctg gaacggtttc agaatgcaga tgtgtttgcg
     1561 gatgcccagg acggcgtgct gattctggag gacgggcagc atcggctgac gctgacggcg
     1621 agcatttcgg cgacggcgac ggtgcaggct ctggtggaag atctgacgga taccgaacag
     1681 gcgcaacccg aacagctgtc ccagctgctg cagctggcgg cggtgcagac tccggatgtt
     1741 tctgtgacag aggacgaccg ctgggtgctg ctctccacgc tggactatgc agatgaaaaa
     1801 agcgaaaacg gcacgccgtc tatgctgctg ctgaaattac aaccggcgtg agcatattgc
     1861 aatgcgacag ggcagggaaa cttgccctgt tttttgtccc tttcagggga atttttctcc
     1921 caatctgccg ggttgacttt gttccggcaa atgtgctata ataaaagtgt tgagaaattg
     1981 gatagatggt ttcacgggct gaatttgcct gtgatattgg aaacgtacat tctccggaat
     2041 gattcacgaa caacacgcta tgccagaacg gcggcactgc acggtttcgg tgcaggactg
     2101 ccgaaacctg tctgcatcgg tacgcagacc ggaatggctg ggtgcaggtg cgaaatttat
     2161 tctaggagga tttttttatg cccaaaaaca aagtgcagga agttattttt accgttctca
     2221 tggtgttcgt gatggtctac gccatgatct gctacaacat tgccgtagag accggcggca
     2281 tgacgaatct ggtctttctc catgcgttcc gggaactgct gattatggga cccatcgcat
     2341 ttctgctgga ctttttcctg gtgggcggtc tggcgaagaa ggcggcgttc cgcatcgtca
     2401 acccggaaaa ggacaacccc tttcacctgg ttctggcgat ttcagcggtg tcggtgctgc
     2461 tgatgtgtcc gttgatgagc ctggcggcaa ccctgctgtt caagcacgcc ggcgtgcaga
     2521 ttgtcgctgt ctggtttgag acaacggcaa agaatttccc catggcactg ctctggcagc
     2581 tgttctttgc cggtccgctg gtgcggttcg tgttccggca tctgatggca ctggtgcagc
     2641 cccgggaaaa ggcggctgtc acagaaaagg cttgatgtag aacgcaaaaa tcccgttcca
     2701 ttctgctgca catacagcag tgtgggacgg gattttgttt gacatttttc gcagagtatg
     2761 ttatactaaa actctgaggg catacctaaa acggtaggtg gtttgtttct gctcccactg
     2821 ctcatttgaa cagatgggag gtgatcggta tgatggatca ttttatcacc ctgggtgact
     2881 tgttgaaact cggcagtttc ctgctggatc taacaagtgt gatcttgtac atttactaca
     2941 atcaaaaaaa tagtaaaaag catcaccata aaaagaaaaa gaaataaccg ccccaagcta
     3001 ccaaactgtg gcggttactt ctttttaacc taagcagtgg gagcagaccg tctactggta
     3061 tgctctcttt ctacttttat catacatcat tttcgccgga atgtcaagtg gttttttcca
     3121 cgttctttca aaatgcggat gccgcaggga aacgctcaaa aaatctgtgt aaaattgtaa
     3181 aaaagtactt gtattttgct tgttcctatg ctataataaa agtactagcc agggatacga
     3241 gcctggcaaa tcttactatt ttacgaaaag gagtttcccc atgaaatttg gctattttga
     3301 tgacgcaaac aaggaatacg tcattcagaa tccgaagacc ccgtacccgt ggatcaacta
     3361 cctcggcaca caggagttct tctctctgat ttccaacact gccggcggct atcacttcta
     3421 taaggatgcc cgtctccgca gaatcacccg ttatcgctac aacaatgtac cggtagatat
     3481 gggcggtcgt tacttctaca tcaaggacgg cgagaccgta tggtctccgg gctggtctcc
     3541 ggtaaagact gatctggatg cttacacctg ccgtcacggc atgggctata ccgtaatctc
     3601 cggcaagaag aacgacatct ctgctgaagt gactttcttc gtaccgcaga actacaacgg
     3661 cgagatccag agcctggttc tgaagaacga gggcagcgag gcaaagacca tcaagctgtt
     3721 ctccttcatc gagtggtgtc tgtggaatgc acaggatgac tccaccaact tccagagaaa
     3781 cttcaacacc ggcgaagtag agatcgaagg ctctaccatc ttccacaaga ccgagtataa
     3841 ggaacgccgc aatcacttcg cattctacac cgtaaatgat tccattgacg gttatgacac
     3901 cgatcgtgaa agcttcctgg gtctgtacaa cggctttgag aatccgcagg tcgttgcagc
     3961 cggcaaggca tccaactccg tagcagacgg ctggtctccg gtagcatcac actataagga
     4021 aatcacactg gctccgggcg aatccaagca gctgatcttc attctgggct atgtagaaaa
     4081 cccgaaggat cagaagtgga acgctgacgg cagcatgaac aagtcccgtg catacgagat
     4141 gatcgaacag tacaacacac cggaaaaggt tgctgctgca ctggctgaac tgaaggcaat
     4201 gtgggatgca ctgctgtcca agtacagcgt caagactccg gatgacaaga tgaaccgcat
     4261 ggtcaacatc tggaatcagt atcagtgcat ggtcaccttt aatatgtccc gttctgcatc
     4321 ttacttcgag agcggtatcg gccgtggcat gggattccgt gactccaacc aggacctgct
     4381 gggctttgta caccagattc cggacagagc aagagagcgt ctgatcgacc tggcatctac
     4441 acagctggaa gacggcggat gctatcacca gtatcagcct ctgaccaaga agggcaacaa
     4501 cgagatcggc ggcgacttct ccgacgatcc gctgtggatg atcctgtctg tggctgcata
     4561 catcaaggaa tccggcgact acaccatcct ggacgctatg gtaccgtatg acaacgacga
     4621 gtccaaggca aagaccatga tggatcacct ggagaagagc ttcttccacg tggtagaaaa
     4681 cgttggcccg cacggactgc cgctggcaat gcgtgcagac tggaacgact gcatcaacct
     4741 gtcctgtttc tctgacgagc cgggcgaatc cttccagacc tctacttctc cgaagtacgg
     4801 cgaggataag tattccaagg ttgcagagtc tgtaatggtt gctgcgctgt tcacttacgc
     4861 tggtccggaa tatgtggcta tgtgcaagaa gaagggcgac gaggctggtg ctgcaaaggc
     4921 acaggctgcc atcgaccaga tgaagaagaa catcatggat tacggctggg acggcgaatg
     4981 gttcctgcgt gcgtatgatg acttcggcaa gaagatgggc tctcacgagt gcgaagaggg
     5041 caagatcttc atcgaaccgc agggctttgc tgttatgggc ggctgcggca aggagaccgg
     5101 cgcagacttg aaggctctgg agagcgtaga caagtatctg aattctacac acggtctggt
     5161 tctgaaccag ccggcattca ccaagtacta catcgagtac ggtgaaattt ctacttatcc
     5221 gggcggctat aaggaaaatg ctggtatctt ctgccacaac aacgcatgga tcatctgtgc
     5281 agaggcattt gttggtcacg gcgacaaggc attcgaatac tacagcaaga ttgctccggc
     5341 atatcgtgaa gagaagtact ccgacctgca ccgcaccgag ccgtatgtat atgcgcagat
     5401 gattgcaggt aaggatgcaa agcgtcacgg cgaggcaaag aacagctggc tgaccggtac
     5461 tgctgcatgg aacttcgtag ctgtttccca gtacatcctg ggtgtaatcc cggattggga
     5521 tggtctgaag atcgatccgt ccattccgca cgaatgggat ggcttcacca tttccagaca
     5581 gttccgtggt gcaacctatg atgtcaccgt caagaacccg aaccacgttt gcaagggtgt
     5641 tgtatctgtt acagttgacg gcaaggcaat cgaaggcaac gtactgccgg tattcagcga
     5701 tggcaagaac cacgctgtag aagttgtaat gggctaagct gaaacagttt tgaacaatag
     5761 aacgatttga gtaaatcgga aaaatttcct ttcaaatcag aaaagggact gccatacaaa
     5821 cggcagtctc ttttcgtttt tgtgcgtatt tgcggcgtgg attgcatctt ctcctagaga
     5881 aaatgccctg ccgaaaaaat cgtaggggcg aacatcgttc gcccgagact ccgaccgcac
     5941 tgcgtgccac ctcctctgta gaggaggctt ttctaaatag tctcctctca tagaggagat
     6001 gcccgacagg gcagaggagt ctttgatccc ttgaaaaggt aaatctgtag gggcgaacat
     6061 tgttcgcccg agactcctcc ggcactgcgt gccacctcct ctgtagagga ggcttttcta
     6121 aatagtctcc tctcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagag
     6241 gagatgcccg acagggcaga ggagtattca gtctccctt
//
LOCUS       DAWENO010000118         6195 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_4995_length_6195_cov_9.857980, whole genome shotgun sequence.
ACCESSION   DAWENO010000118 DAWENO010000000
VERSION     DAWENO010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4995_length_6195_cov_9.857980"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3649..3658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4149..4158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1451 a         1707 c         1486 g         1531 t
ORIGIN      
        1 actacggtga cggcgcatat cacacgggct ggttctatca gaacgggaaa acatactatt
       61 tcaacgatgt agacggcgcc atgttcaccg gatggcagac catcgacgat cagacctatt
      121 acttccaaga agacggtgcc gccaaggtcg ggtggctgga gctttccggg aaacgctact
      181 atttcgataa agaggcaaag ctctgcaccg gatggcagac catcaacgga aagcgctatt
      241 gtttccgcgc agacggctca gcagtgaccg gatggcaaac cgatgccagc ggctccagct
      301 attacttcct ggacgacggc acgatggcga ccggctggac ttccgttgga aagaacacct
      361 actatttccg caaggacggc gtactgatgc gtggggtcac cgagatcgac ggcaagcagt
      421 atctgctggc agaggacacc ggcatcctgc aaaagaatac caccatcaac ggcattacca
      481 cggacgcaaa cggcgttatc atcaagcgca tcctgtccgt cccctatctc agccaaagcg
      541 gttttcccac tggctgtgaa agcgccagtg cagtcatgct gctgcggcag gcaggctaca
      601 gcaccagcat tgccgatttt gttgacaaat acctggacaa gggcagcttt tactggaaaa
      661 acggcacaca atacggcccg gatcccagca caaaatttgt aggcgatccc cgttcgtcca
      721 gcggtttcgg ctgctatgcg ccagttatca ccaatgcact gaataaaatt ctgaccaatg
      781 ggaaaactgc aaaaaacatc actggcacat cgttctcgtc tctgctgaca cagtacatcg
      841 acaagggcat tccggttgca gtatgggcat ccatcaacat gatgcagatt gacaacggca
      901 gacagtgggt cgttccggaa accggaaagc tgttcacctg gaaacggcac gagcattgcc
      961 tggttctggt cggctacgac aaagactact actacatgaa tgacccgtac cagagcaagg
     1021 gactggttgc ctataagcgc agcgtggtag aggcacggta tgccaccatg ggttcccagg
     1081 cagtcgtaat ccagtaagca cttacatacg tctatcagca acacaaaacg gagggatacg
     1141 atgaaacttt tatttatccg ccacggcgat ccggactatg aaaaagactc tttgacagaa
     1201 aagggatggc acgaggcaga actgctggga cagcgcatgg cgaacacgcc ggtcactgcg
     1261 ttctacgtct ctcccctggg acgtgccaag gatacagcat ccaagacact gcaaaaagtg
     1321 ggcagagatg ccacagaact ggcgtggctg cgggaatttc acgcacccat tccggatttt
     1381 cacaccggcg aaccaagaat tgcctgggat cagctcccgg cagactggac cgcagagccg
     1441 aagtattacg acaagacacg ctggtcggaa accctgccca tgctgcaggg tcacgtgatc
     1501 gacgaggcaa agcgtgtctg gaacggtctg gacgagatcc tgacacagca cggctatgaa
     1561 cgggaaaaca acttttaccg tgcagttcag ccaaacgaag agacgctggc gtttttctgc
     1621 cactttggtg tcacctgtgt catgctgagc catttgctgg gcatctcccc tatgatcctg
     1681 tggcacgggt tctgcgctgc gcctacgtcc gttacttcgc tgattaccga ggagcgccgg
     1741 gaaggcgtgg ctctgttccg gatgaacgct ttcggagaca cagcgcatct ctacgccaac
     1801 caggaagagc ctgcattcgc tgcgagattc tgtgaaatgt atgcgaacca ggaacagcgt
     1861 cacgactgac actgacaatc aaacagcaat tacgcaaaaa cggcactttt cgaaaagtgc
     1921 cgtttttctt gttatgcttt tttagaaaga aaaaagcagc cttatttatc acaaataagg
     1981 ctgcaagttt ggcggacagg gagggattcg aaccctcgat acgctattaa cgtatacacg
     2041 agttccagtc gtgcgcctta gaccagctca accacctgtc cagttattct gcttcctgcg
     2101 atttgaggaa gtggtgcgag tgacgggact tgaacccgta cgccgaagca cacgcccctc
     2161 aaacgtgcct gtctgccaat tccagcacac tcgctcttca tgtttttcat gtgtcgcatt
     2221 cagctttatt attatagcag aaaaacagag gtctgtcaat agtcgaaatc actttttatg
     2281 tttatttttt gtaaatataa agtgaatttc agagaagaac cgttttttct gcgtttcata
     2341 ccccgggaca gaaacaaaaa agcagattcc atgagggatt cccgcacgaa atctgctttg
     2401 tgattctttt caatacagtg cttacagcat ctcagctaca tcatctgcga tctgttctgc
     2461 tgtcagatga ttctctttca gaacttcctg tacattataa cgatcgagga attctttttt
     2521 cagaccaaag cactttgtct ttacggcaga gtcgccgtaa aaccgggcga tcttctcacc
     2581 gaatccgccg tccagaatgc cgtcctccag cgtaatcaca accttgtgat ctgctttcag
     2641 ctgttccagc agtgcggtgt cctgtccggt gatataatac gggttgataa cagtcggtgc
     2701 agtaccggtc ttttcccgga tcttctcggc tgctgccaca cccaggtgat agaacgaacc
     2761 cagcgccaga attgccacgc cgcttccctg ctgtgtcact tcatagcggt tcagttctcc
     2821 gaagtccttt gtcactgcct tgccgtccga aacatactgt gccggcacac ggatcgcaac
     2881 cggatgttcg ttctgatcca gcgcccaatc cagcattgcc tgataatcct ccaccgttac
     2941 cggcgccaga taaaccaggt tcgggatgtt ggagatcatg ggaatatcgt agatgcccag
     3001 atgtgtcaca tcattcatgc caaagacaga ggctgcaaaa acggagatcg tcacagcgct
     3061 ttgattgatg cagacatcct gggacagttg gtcataggtg cgctgtaaga aagaactgta
     3121 tacgccccag aacggctttc cgccgttttt cgcaataccg gatgccatag cggcagcctg
     3181 ctcttctgca atgcctacat caatgaactg tttgcctgcc tttttccgga tatcttccgt
     3241 aaatccgaat acggtaggcg tagcagaagt aatggcacag acacgcttgt cctctgccat
     3301 cttcttttgc aggtactcac aggtaaagct gccgaaatcg ccgtcaccgc cgaacgaaac
     3361 cgtagtcttt ccggtagccg gatcaaacgg catacaccag tgccactgct ccttgtgctg
     3421 ttctgccagt tcatagccct tgcccttctg tgtcacgatg tgtaccacta ccggctttgt
     3481 gctgtccttg acctgacgaa atgcagcgat cagagacggg atatcgttgc cgtccttcac
     3541 gaaaacgtag tccagtccca ttgcccggaa caggttgcac tctgccttgc cgtctgtatc
     3601 ccggaggagt ttcagattct tgtacagacc gccgtggttc tctgcgatnn nnnnnnnntt
     3661 gtcattgacc acaatgataa agttggtgtc catctcgccg gcaaagtcca gaccctccag
     3721 cgcctcgcca ccgctgagtg agccgtcacc gatcacagcg atcacattgc cgctttcgcc
     3781 gttcagatca cgtccctttg ccagaccgct ggcaaggctg acagaggtgg aagtatgacc
     3841 cacggtaaag aaatcgtgct tgctctcatg cggatcggta tagccggaga tatcgtcaaa
     3901 atgttcctca tagagatatg cgtcttttct gccggtcagc atcttgtggg gatagctctg
     3961 gtgggaaacg tcaaaaacga tcttatcctt cggggaatca aacacatagt gcagtgcgat
     4021 ggtcgtctcc accatgccga agttcggccc gaagtgaccg ccgtgcttgc tcagacggaa
     4081 catcaaagcc tggcgcattt cagccgccag cgcattcatc tcgtcttgat tcagtttctt
     4141 cacatcttnn nnnnnnnncc ggtgcattga ttttttccag atacatggtt ttcctcctaa
     4201 tcgttttcat tcttcgggga acatttgtcc caggtgagtt ttccggtttt tactgcctct
     4261 tcatagcggc tgatcttata attcagccgt tccagcgtga cgtcgatctg tgccttttga
     4321 tccagcagag catcccgctg atccagcagc agctgaagcc gtgccggaat ggtattgtca
     4381 ccttcctggc aaagcttgac gtattcgatc atcacctcca ccggcagacc ggcattccgc
     4441 atacaaatgg caagctccac ccagcccaga tcatccggct ggtagtcccg gttgccgctg
     4501 ggcgtccggg tcactctggg gatcatgcca acacgctcat aatagcgcag cgtgtcctgc
     4561 gaaatgtgat aggtttcact gacttccttg atggtcatgc tttctctcac ctcctgcaag
     4621 catattatag cacctggagt cagctccaag tcaataccca aacgcaaaag tttacatttt
     4681 agacacaacg caaaaagcac tgcggaacgg ttttgttccg cagtgctttt tttggattat
     4741 gattttattg tctgataaaa attcgggttt cccagcggtt ctgctgtcag acggaatatc
     4801 acacagccat cgggacaaac cacagtcttg ctgtattttc cgtcgccgcc aagatttctc
     4861 aaatcgccgc cgctgcgcac tgcatccatc agcggataca acagtagcat gaccttgctg
     4921 catatcccat agccatctgt attcaccgaa caaccatagg tacagcagta gcgatctccg
     4981 atctcttccc cgtttcgaca gtaattttcc gtctgttcac tctgaaggaa tccggtcacc
     5041 gttatggtaa atgcaaattc ttctgcaaac cattttttca ttgctgttcc tcttttcagt
     5101 ttgtctgctg aatcgaatca attttccact gtccgttttc caaaacgatc tgcgccgtca
     5161 gcgttgcaga tgcgccaaag tcgtcatact gcatggatgc agtgaatgcg ctatctgaga
     5221 gatccgagac ctctgctttt ccggaagtcc aggagaatcc gccgctggtg gcaaactgcc
     5281 tgccgtacag actgccgttt gcgtcaatgt acagcggctg gtctgtaccg agaatcgctg
     5341 catagcggct gtcgatcatc ttctgggtca agctggattc catgtaggct ttcagatctg
     5401 ctgtactgga aaactgggtt tttgccacct tctcataaac agcgcccgac ccggtcgaac
     5461 gaatggtatc actttcgtcc ttcggcagat tgccggcgcc gatctggtcg atataatcca
     5521 gtgcgccgaa caggcgttct gccgtttctt cataggcaga tgtgtccccg gcgctctgtg
     5581 agacggctgt ggtctccgct gccgtggtct cactgctttc ctgtgccggc agcgtctccg
     5641 cagccgttgt ctttgtatcg gtggtctctg ccgtctcttc cggggcagcc gttgtttctg
     5701 cctgggtctg ttctgcggtc gtctctgtgc tgcgctccgt cagactggac gagggtgttg
     5761 tgatctcgcc gcagccggaa acacccgtca gcacagctgc tgtcagcaac gcagccagcc
     5821 aaaacttttt attcgtcttt ttcatctttg ctttcctcca aaagaatgcc tggcaagtca
     5881 gccttttttt catgatattc cttgagtttc tgattcattt catttgccgt ctttttgaat
     5941 acctgatagt tcagcgcaga cgtgaaaaaa taaaccgctg cactggtcag acacattccc
     6001 agaacgccaa aaaaattcgg agtgtaccaa ccgtaaaaat atccgcacac cagtacagat
     6061 accgtaacca gcacatagtg aatggtcagc cgtatcatcc cctcccggtt gctcacgtcc
     6121 ttatggggca ggaccagctc cgtaatgccg cctgccagca caccaatgga caagatctcc
     6181 ggcaatactg catac
//
LOCUS       DAWENO010000119         6167 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5024_length_6167_cov_8.508017, whole genome shotgun sequence.
ACCESSION   DAWENO010000119 DAWENO010000000
VERSION     DAWENO010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5024_length_6167_cov_8.508017"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1488 a         1466 c         1681 g         1532 t
ORIGIN      
        1 cgccagctcc atgcggcgag ccgggactat atcgcaatga cagtcacagt gctgctgttt
       61 gctgcggcag ttgctgtcaa aatcatctgg taaggagaca ttttcatgaa cgcaacgctg
      121 tccgagacaa ccgcctccgg aaaacccgga aagaaaggct gcggaagatt ggttttgctg
      181 ctgtcgtttt gcatcccgat gctgcttttg gcggtgggat ttgtcctgca gcaggtacac
      241 ccctttggag acagacaaat actggtgaca gatttctggc accaatatta tccgtttctg
      301 cggctgctgc atgaaaaact ccggagcggc ggctcccttt tgtatacctg ggaatccggt
      361 atggggacaa actttctctc catttttgcg tattatgcgg caagtccgct gaatctgctc
      421 accgttctgg ttcctgaatc cattctgcgt gaagcagtca cagtggtact tctcctgaaa
      481 atcggctgtg ccggtttgtt ttttgcgctg tttctccgtg ggacatttca cagagatgac
      541 ttttccatct gcctttttgc tgtgatgtat gcgctttgca gctacatcct gggctactac
      601 tggaacatca tctggatgga tacagttgca cttttgccgc tcgtggtgct gggattggtt
      661 tacctggtgc gtgacggaaa ataccgtctc tatgtggtcg ctctgggact ttctcttttc
      721 accaactttt atatcggtat gttcacctgc attttcgcag tgatcgccta cgtgtgcctg
      781 tgcgtgttct atctgaaacc ggcgcagctg ccgggacgta ccattgcaat gctgctggga
      841 tctctgctgg gcggtgcatt ggcagcaatc gtgcttttgc cggcgtacta tgcgttgcag
      901 ctgacctaca gcgtcaacaa cattttcccg accacggtgc agttctatga gagctggcgc
      961 acgcttgcgg cagacctcat cagtttccat gaaccgacgg caaaggacgg cttgccgaat
     1021 ctggcttgcg gcgtgctgtc gctggcactg atgggaccgt ttctgcgctc tgcatctatc
     1081 cggatacggg aaaaagtcgg ggcaattctg gtgctggcat tcctgcttat cagctgtaac
     1141 tgcaacgtgc tgaactacat ctggcacggc tttcacttcc cgaatatgct cccatatcgt
     1201 ttttcgttct tgttctcctt tgtgctgctg actgtaggct accgtgcctt tcttgcggca
     1261 ttggaagaaa agttcaaggt ctgggacatt ctggcgatgc tggttatggc tgtgctggta
     1321 tttgccgtct cctacaatgt acaggaaaac caggcggtgt actggtctgt ggcagtcatg
     1381 ctcctgtacg ctgtgatcct gttgctgttt ttccgggaga tctttggaaa aaaactgctg
     1441 tatctttcac tttccatcgt tcttgcattt gaaatgttcc agaatgtaaa acttggaaca
     1501 gaaaccgtgt ccacctcaga ttacctcagt tacccgacac agcatgaaga agtggaatca
     1561 ctgctggcgc agattaggga acaggatgac agccttttct atcggacaga gctttcttcc
     1621 tggtacacgc tgaatgaccc ggcgctgtac ggttatcacg gactgtcgca gttttcttcc
     1681 acagcgaatg agagtgtgac aaagtggatg cgtgctatcg gcgttccggc gtctgaggca
     1741 gggaaccgct actactatgg cggcggtacg ccggtcagca atgccttttc cggaatccgc
     1801 tatctaataa gccggagcgg ttctgtgctg gatttgcagg aatggcagca gcttgcatct
     1861 gagggagcgt gctattccta tcagaatcag tatgatctgc cggttggatt ctggacagca
     1921 tcttcgctgc aggactatac cgttgtcccg gacggcaatc cctttgacaa tcagaatacc
     1981 ttgttccgtc tggcaaccgg agttgaaacg ccgctgttca cccggatgga ggtagacagc
     2041 gttttgtatg agggtgcaga tgccacgaaa aacggctacg gcagctatac ctaccaggca
     2101 gacgccggcg cagaaaccag aaagctgcaa tataattata aactcccggt ggacggaacg
     2161 ctgtatgggt atatgaatct tcccaatggt tccagcattt cagttctgcg caacgacagt
     2221 tttgccgggt cgtataacaa cggcaaccag ggctatattt tccccatggg aacattttcg
     2281 ggcagtgaga cagcaagcgt ttccgtttca ctgccggcgg attctgtcac cggaacagtt
     2341 acggtctatg tgtatcagct gaaccttgca gccctggaag agggttacgc taagctgcag
     2401 tccggtggaa tccagatcac agaatttaca gacacaaaac tcagcggttc actcaccgca
     2461 tcacaggatg gtctctgcta cttttccatt ccgtatgagg aaggctggca ggcagaggtg
     2521 gacggtgtca aaacagcgat tacgccaatc ggtgatgcca tgatttctgt gccggtcaca
     2581 gccggaacgc ataccgtttc gctgcactat tgcccgaagg gctttgcggt aggggcgtgt
     2641 gctacaactg gcgcagtgct gttgctcatc gttttggcgg tgatcgaaca taaacgaggg
     2701 aaaccttttt tgcagcctgt ggcagcgcaa cagaacgtaa aagagataat acataacgat
     2761 gcggaggaca cggaaacatg agaaatttga aagtgaccat gggattccgt gggacaaact
     2821 atcacggatt ccagcgtcag cccaatggaa tcacggtgca ggaagtggtg gaaacagcgc
     2881 tttccaagct tttcaatgaa ccggtcacca ttcagggctg ttcccggaca gacaccgggg
     2941 tacacgcaaa agaatactgc tttaccatgc agacggaaaa gcagctgccg gagcgcaact
     3001 ttatccgtgg cgcctgcggc tatctgccgg aggatatttc catttatcgg gtggaagaga
     3061 tgccggcgga ctttcacgcc cggttcagct gtaaggcaaa agaatatatg taccggatcc
     3121 acaacagcga aagcaagaat ccctttacaa cagatctgga actgctctat cgccgcaagc
     3181 tggacattcc tctgatgcag gaggcggcac agcattttat cgggacacac gactttgcaa
     3241 cgttttgtac aaactgcaca gaaaagcaga atacaattcg tacaatctac gacattacca
     3301 tagaaaagca ggggaatacc gtgattctgc ttgtcaaagg gaatggattt ttgtataata
     3361 tggttaggat tctggtgggg acgctcctca gcgtcaatga aggacggatc gccgtctcag
     3421 aactggatgc acttctggca tccagaaatc gtgttctcgc cggaatcacc gcacaggcac
     3481 atggtttgta tctgaaccgg gtattttatg aggatgaatt ttagggaata aattccagcg
     3541 cctgtcggaa aggagaagat caaaaagctt atgaatcatg attctgatga tatgatcgaa
     3601 aagcgaaagc gccgcagcaa actgcgtgca tttcgcaact tactgttatt tctgctgatc
     3661 gtcggctttt gcggctatct gtatgtacag cgggatatgt ggattcccaa gctggagggc
     3721 atcggcagcc gctatgattc cgtgacacag aacgacggca cgttggcaga gggcaacttt
     3781 ccgctgacca tctccggcag ctccagctat caggcagaca ttgtagatga cacactgttt
     3841 ttgctgcacg atgcttatct ggatacctat tctctgcacg gagattccgg cgacacccgg
     3901 cagcacgcat ttcagaatgc catgatgtgt tccggcggaa aatacgccct gctgtatgag
     3961 agcggcggca cttcgttccg tctggatacc aaacataaga atgtctacag caagtcagtg
     4021 gacaacgata ttatttccgg tacggtcagc gacaacggaa cagttgcact gattacggaa
     4081 tccagttcct atgcttgttc tattttggtg tacgacaata caggaaagcg catctatcag
     4141 cgaaactgcg tggaatatgt ggtagacata gcgttccaca aggacaacga cggactttgc
     4201 tttacgtctg tccaggtgga aaacggtgtg atgaacagta cgatcaccag cgtactgttt
     4261 caccaggtgg atatccagtg gacttccatg ccgctggaga caatgagcgt aaaatccggt
     4321 ttttcttcga atgataagct gtgtgtcgtg ggaaatacag cgtgtgcata ttatagcagt
     4381 accggcgaaa tgctgggtac gtataattat aatggtacac tggtcagtgc agatctggaa
     4441 aacggcaaag cggcactgat tatccaggac gaccaaaagc ggaatacctc gcttgttctt
     4501 ctggatcagt cggtctccag cccgaaggtg atatccattg acagtagcgc cgaactggtg
     4561 cgtgtctttg acggagatgc catcgtaatg agtccgggaa acatcacttc ctatgccttt
     4621 tccggagatg ccattgcgac cgtaaagctg gagggtgcat atacctcttt cctcaagcag
     4681 gacggatacc tcttcctgtt gggatacgac cagatcgacc gtgtagactt taaggagtag
     4741 tacatggaaa cttggatgaa tcagacatgg tggctgtacg atctgacagt gattgcaatt
     4801 gtgacgctct gtatctgggg cggatggcgc aggggactga tccgaactgc tgccggtctg
     4861 ctcggctacg gcgctgcact gctgctgtcc ggcgttctgg cacagccggc ggcagaattg
     4921 gtttacaatc gctggctggc agagccatgc gctgcgattc tggaacagaa gatggagcag
     4981 taccatttgg cggacagcat ccagcagaca ctgaacgcat acggtgtgca gctggacgat
     5041 gcgtcattgc agcagattgc cgatcatccg gacagtgcga ccgatcagct gtacacagtt
     5101 gtgtctcaga aaaccggtct gcctgcggaa ctgctgcggc agggactgtc gcaaagcatc
     5161 gacagtgccg catcgcagat gtttaccggg ctgcccgact ggatgagcga ggcgattttg
     5221 cccaaacagg attcgcagga actgcaaaac cgtgcggtgc agtcggcggc attggtgctg
     5281 tccggtgata cgcaccaggc ggcagcaaaa ctgacagaga tgtacgtgca gcctgtagta
     5341 gtttcgattg ttaaaacatt tactttttca gttctatttc tcttgatttc tgctctgatt
     5401 caaggtataa taaaagggct gtctgcgatg cgccgcacag aaagcggtca tttgacagac
     5461 cagacgctgg gcgccgcagt cggcggcgtg caggcggtgt tcctgctggt gctgatggga
     5521 aagctcacgg ggtggcttgt ccgtcaaggc ggcgatcagc ttgctttttt caacgaagcg
     5581 atcatccgaa aatcagtttt atttcagtat atataccaga ttgcgttcat acagaagtga
     5641 ccgtatttct gtatgaatcg acacaaaaca aaaataggaa aggtgtgtag aaaatatgat
     5701 gctatgcagc agatgcaaaa agcgtcctgc ggttgtcttt atcacggcaa tgcagggaga
     5761 caaaaagaaa aacgatggtc tgtgtctggt atgtgcaaag gaaatgcacg tgccgcagat
     5821 tgatgaatat atgaaacaaa tgggaatctc tgacgaggaa ctggaacaga tctcgaatca
     5881 gatgatggac atgatggatg gtgacagttt tgaaatggga ggttccggtg tgatgccgca
     5941 gtttttgcag aacatgatta aggataccgg aaaggcattg gaaagcctgc aaaacggggg
     6001 agtgccggca aagggtgacg aggatgtacc ccaggcagac tataccgaag ttccggattc
     6061 tccccgccgg gagcgccgct ccaagcggaa aaaggagctg aaattcctgg ggaactactg
     6121 caccaatctg agccagcgtg ctgccgacgg aaaactggat gcagtga
//
LOCUS       DAWENO010000120         6155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5034_length_6155_cov_11.367377, whole genome shotgun sequence.
ACCESSION   DAWENO010000120 DAWENO010000000
VERSION     DAWENO010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_6155_cov_11.367377"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3808..3817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1510 a         1578 c         1345 g         1712 t
ORIGIN      
        1 tgattcccgg attgtcctgg tagatcttct tgattttctt gtcgtctccc ggtgttgtcg
       61 ttttgatgtt attgttgccg ctgcgggcaa tcacatttaa ttcgtcattg gtgttgttta
      121 atgtcggctt atgtgcacgg acagtttcaa gggttgcatt gggattgctc tttttgaaaa
      181 tctcgaaggc atcctcatcg ctgattcccg gattgtcctg gtagatcttc ttgattttct
      241 tgtcgtctcc cggtgttgtc gttttgatgt tattgttgcc gctgcgggca atcacattta
      301 attcgtcatt ggtgttgttt aatgtcggct tatgtgcacg gacagtttca agggttgcat
      361 tgggattgct ctttttgaaa atctcgaagg catcctcatc gctgattccc ggattgtcct
      421 ggtagatctt cttgattttc ttgtcgtctc ccggtgtgat cgttttcata tcattgccgc
      481 tatgggatgc aattgtattc aattcctggt tgctcagatc tttgctctta ccgctttctc
      541 ccgacttttt actctgactc gccgtatttt tcttgggttt cgatttgccg cttgtctggc
      601 tgttgtcggc gatgaggtca atgcccttgg aaatgttatt cgccgttttg ctgtcggacg
      661 aagtaaagag aacaattgcg atcaaaagca caataaaaag ggcaaggggc agcacgttca
      721 gaattgccgc aacgaccagc tgaaacatgg gcttgccgtt tttctttttg aaacaagcga
      781 cacaatacgg aaagaaggag ctgtcttcct tggcgctttt cttctccttg acgatctgta
      841 caatggtgat gatctcaaag atcacgcccg aaataatcga caaaattaac gcaaagttat
      901 tgccgtccgc cgaaccgctg gcagcggcgg caaacagaat aatcatgccg atcaaaaata
      961 aaaccatatt gacaatggaa atcccaaaaa ggatggtgtt ctttttcata atatacctcc
     1021 gtgtacctat ctcatagttt tgtcgggacg ctggctttca tcctgcctgc cccgggttgt
     1081 ttctgcccac aaaattgatg ccgctttcat cctccctgtg ctgttttttg cgctgaaccg
     1141 attcacctgc catttcgcat cgttttcact tttttctctc ttacaattgg atttttccag
     1201 caaaaacgtg tatattctta ttataagcta tattttttcc gttgtcaagt cgtttttcaa
     1261 cttttcatct gtttacattc agaaactgct ccgatactct taatagcaga ggtgataaca
     1321 catgatactg gactatccgc aaatcggaaa aaatcttgca aagcgacgaa aggagctgaa
     1381 actaaaacag tacgaagtct gtgagcgtgc ggagatcaat gacaaatacc tttcctgcat
     1441 tgaaaccgcc cgctctattc ccagtttaga agttttcctc agactctgta ccgctttaga
     1501 aacaaccccg gaccgtattc ttttcggcac tgtccgccaa acagattaca cgaaagacag
     1561 tacgatcctg ctggaaaagt ttcagcggct ttctcccaac aacaaacgtc tttgccttgc
     1621 attcatggac ctgctttcca atgactttca gaatatgtag tccatgcttt tattatagtc
     1681 aaagagccag acagacgttt gtccgttcaa aaacaattta cagccagacg caaaaaacag
     1741 ccgctctgca tcccgcagaa cggctgtttc cttttcaaag gatgtctttt ctgtttttag
     1801 aatacttaca tcaaatcgca gatacggttc gagaatgcca ccggatcttc taccggcata
     1861 ccggcgatca gcagtgcctg gtcatacagc agctttgcat actcgtccag ctttgcggta
     1921 tcgctggtct ggagttccat cagacgctga tagatgggat gctccggatt gatctccagc
     1981 acggtctgtg cctggagttt ctgaccttct gttcccggca ttgcgttcag cgtctttgcc
     2041 atttccatgg aaagttcgcc ctcgctggac aggcagaccg ggtgggactt caagcggccg
     2101 gacagcttga cattgccgac cttgccctcc agtgccttct tcatttcctc cagcatatcc
     2161 ttgctggatt cctgtttctc ttccagttct ttcttttcct cttcggattc cagatccaga
     2221 ttgctgtcca gaaccgactg gaactgtttg tcttgatagt tctgcatcat gcggactgca
     2281 aactcgtcca cattctcggt gaagtacagg atctcatagc ccttgtcctt gacctgttcg
     2341 gtctgcggca gggactcgat acgtgcaacg ctttcgccgg ctgcatagta gatcttgtcc
     2401 tgtccctccg gcatacggtc tacgtattcc ttcagagaca ccagcttgcc gtcctttgag
     2461 gacgggaaca gcagcagatc ctggagcaga tccttgtgca taccgtaatc ctggtagatg
     2521 ccgtatttca gctgcaaacc gaaggactgg aagaacttct catacttctc ccggtcgttc
     2581 ttcatcatac gctggagttc gttcttaatg gtacgctcaa tgctacgtgc gatctggcgc
     2641 agctgtgcgt cgtgctggag catttcacgg gagatgttca gcgacaggtc ctccgagtct
     2701 accagaccct tgacaaagct gaaataatcc ggcagcagat cctcgcagcg ctccataatc
     2761 agaacgccgt ttgcatacag ctgcaagccc ttttcgtagt ccttgctgta atagttgaac
     2821 ggtgtgtgcg acggcacata cagcagggca tcataggtga tcgtaccctc gttccggacg
     2881 tgcatatggc acagcggatc ggtataatcg ccgcacttgt ccttatagaa gctgttgtac
     2941 tcctcttcgc tgatctcatt cttattctta tgccacagcg gaaccatgct gttcagcgtg
     3001 acgttttcga tatagtcctc gtactcgtcg gaatcctctt tcttgcggct cttggtcata
     3061 tccatacaga tggggaaccg gatataatcg ctgtatcgct ttaccaggct ctggatccgg
     3121 tactgatcca ggaattcgct gtaattttcg ccgccgttgc cgtcctcgtc tgctgccaca
     3181 tcgtccagca gttccagggt gatctccgtg ccgaccgtat ccttgtcgca ggtgtccatg
     3241 gtatagccct caacgccctc agactgccat acatagccct gtgtctcgcc gaacgccaga
     3301 gactttactg tcacacgctt tgccaccata aatgcggagt agaaaccgac accgaactga
     3361 ccgatgatct ggttgtcctc tgccaggttg ttttccttct tgaactggag cgaaccgctg
     3421 tttgcgatcg tacccagatg ctcttccatc tcttccttgg tcatgccgat accattatcg
     3481 gtaatggtca gtgtgcgtgc atccttgtcc accgccagcg taatcttgaa gtccgacttg
     3541 ttcatgccca cgctgtcatc ggtcagcgac ttaaaataca gcttgtcgat agcatcgctg
     3601 gcgttagaga tcagttcccg gaggaagatc tccttatggg tatagataga atggatcatc
     3661 atgtccatca gctttttgga ttctgcctga aattgtttct gttccatatg tttcttccgc
     3721 attcccggag aaatgcgact cctttcctgt caaagtggtt tgcaatcgtg ccgctttagc
     3781 actcttgctt tttaagtgct aacactannn nnnnnnntat tatagcatat gtgctaattt
     3841 cgtcaagcgt gattttgcat tgtttacaat ttatttacaa aagaaaaagc ggaacacttc
     3901 caagtgaaat gctccgcctc ttttttgtta ttcagttttg ggttattccg cacaaggcag
     3961 cgtatccacc agatgaatca aaaacttcat caaagcgacc acatcccggc tgtccacttc
     4021 gccgtttcca tcgcagtctg ccagcgtaac atactgacat tgggtgccgg tcgttacaat
     4081 tttagacagc agcacggcat ccgacacgtc aatcacgccg tcccagttca cgtctcccgc
     4141 atggtagata gcagggttta caccgtagcc gaggtatgta agcgacaaat ctacataaac
     4201 atccggattc agtaccaaaa gggttgcgat gatattggcg accttgcttt cttccttcca
     4261 caaagtgatc gcaggcaatt tatactgaaa tgtcgtatag atgtcaaagg agcgataaag
     4321 tgtgcttgat tcgccgtccg tattcacttg atatgtctca tccggagaat gatgaaactg
     4381 aaccgcatcc acatattttc tgggaatgcc caattccgca caaaaagcat ccagcgaggc
     4441 agaatctgca ctttctgcac tgtccccata tttttctacc atggcatcac attgttccaa
     4501 cgtcgcataa gcacagcgaa attgaatgtt ttcatggtac gtgtcgcatt cctcaaaaat
     4561 ctgctccagt tccgcatcag gcagcgcata aaactggtca aacgtgtagt tcgcataata
     4621 aggggcatcc gttttctcat ccgacgcaag gtctttagaa gtataagcgg aaaccggcaa
     4681 tgccgcagaa aacagcatta gtgccgccaa aacggcagaa gatatccttg tccaaatttt
     4741 tttcatcagt gaccctcctt aaaaaccgtt gtcattctcc cataaaatcg aaatgacacc
     4801 tcggcttact ttcagtatat catacccacg ggaaaccgtc aattctcact tcgcacaaat
     4861 acacacactt ttttttacaa aatgcacaaa atttaagatt tacaaatctc cgaatttctc
     4921 caagaacttc tgcacgccga acaaatcaga ctgcacggta actgtcagct cacgcagttc
     4981 caggttcggc attaccagat ccggcaccat gatgacacgc agatttgccg cagcggcgct
     5041 gcgaatcccg ttgggcgaat cctccacggc aaagctcagc cccggatctg cgccaatggc
     5101 atcgcaggca atctcataaa tctccggatc gggcttgcca tgttccacct gttccccggt
     5161 gatgatcgca tgaaacagat ccaccatctt tgccgattcc aggtggtgca tggtactttc
     5221 ccgcccggta gaagtcgcca gagccacacg ccagtcctcc tgccgcagcc attgcagtaa
     5281 ttccaccgca cccggcttaa ttggcatccc atccgccaac atttccgcca taatctcgtg
     5341 attgcgcttg tgaaatgcaa ggtagtcaaa gtccgggtaa ttcttctcaa aaaaagccct
     5401 ggtgtcattg cggttcagcc cacggcaccg gaaaatggca tcgtctatct gtgcaaagcc
     5461 catttcctcc gccgcctgcc gccatgcctg atcgccgaca cgctccgtat caaacaaaac
     5521 tccgtccata tcaaaaatga ttgctttcat gctgttcctc ccgttaataa gaattctaat
     5581 acctttatta taaccgaacc gactgcgtcc tgtcaatatg aaaaaagccg ccggtgcatg
     5641 aaaacactgg cggctttgca atggtgggcc atcggggact cgaacccagg accgaccggt
     5701 tatgagccgg tagctctaac caactgagct aatggcccgg aagtgggatt aacgaagaat
     5761 attataccat agagaggata gatttgtcaa gagggttaga aaaaaggatg aaagaaaagg
     5821 gtgaattcag cgaatgagga agtgataaaa aaggataagg atatatataa agtaaaagaa
     5881 gcggcaacga aaaagggagc ctttcgaaat gaaagactcc ctaaaagaaa agccggcatt
     5941 gaccaatttt cccagatcga gatgaccaag tatcgtaggc gcaaaagagc ttaactgccg
     6001 tgttcggaat gggaacgggt ggaacctctt tgccataaac accggcgaac taaaaaacag
     6061 agataaatga agaacaaatg aagtgcgtaa caaaggacgc atgaacaaaa ggaaaagcga
     6121 acgaccgatt agtatctgca agctaaacac gtcac
//
LOCUS       DAWENO010000121         6147 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5043_length_6147_cov_11.145929, whole genome shotgun sequence.
ACCESSION   DAWENO010000121 DAWENO010000000
VERSION     DAWENO010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_6147_cov_11.145929"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4950..4959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1578 a         1531 c         1671 g         1357 t
ORIGIN      
        1 ttctgcggtt gtacttcctt gaagtttgtg aagatttcgg atagcattac aactttaggt
       61 acttacactt tttatggttg ttccgattta cagagtatac tgctgttcaa aaacttgaag
      121 aatattggat atacttcttt ttatcgttgt gaaagtttga gtgatgttta ctacactggt
      181 tcggaagagg actggaatga gattgacata ggaatggaaa atcagccgct tgaaaatgcg
      241 gtacaccatt acaactggaa tcccaacgac ccgattccgg agccgacaac taccacaaca
      301 actgaaacaa cgaccagtac aacaaccacc accacaacaa ccacaacaac aacaactact
      361 atcacgacag aaaaaaacac tacatccact tcggatacaa ctaccaccgc aaccagcgct
      421 acaaccacca catcacaaac caccaacacg accaatacca cggcgaccac ttccacaact
      481 gtgaccacga ctacgaccac cgatgccccg gcgaatgctg tcggcgatgc ggacggagac
      541 ggcaagatcg actccagcga catcttcgac atcatgctct acgtggctta ctgcgctgtc
      601 ggcaaggacg gcggtctcac tgcggatcag atcgccgctg cggacgtgga caagaacggc
      661 aaggtggatt ctaccgatgt gttctatgtg atgtactacg ttgccctcaa gggtgccggt
      721 acacccaaga cctgggaaga gatcatcaag taaagcacat accgcaaatt aaaacagcat
      781 caaaaaacag ctgtattccg gatatttccg gcgtacagct gtttttgctt attttgtagt
      841 ttttctgcct gcggcggttt tccgctttgc ggcaggcttt gctgtggtct gctttggctt
      901 ttccggcagg atacgcagca tgatgcccga gaacggcggc agttccagcg tgaactgcat
      961 cccggtgcgt gtggtgtcac cggtaatcgt ccgcttgctc tccttgacgc tgccgtggaa
     1021 acgctcccag tccgtgtgca gcagcggctt cagtccgccg cagtccggca gggtaaaggt
     1081 gtagttctgg gtcgcctcgt ccgacatatg gagcaggatc aggatgcggc tgtctccgga
     1141 gcggcgctcc atggcgtaga tgcctttctg ctcctggtgg cagtccaccc agcagaatcc
     1201 gtttcgctca tagtcgtccc ggtacagcgc cggctcggtg aggtacagct tgttcagttc
     1261 caacataaag tggtagaacg catcgtggat gggatatttc agaatgtccc agtcctgctg
     1321 ctgctcctcg gtccactccc ggagctgtcc cagctcgctg cccatgaaca gcagcttttt
     1381 gccggggtgc gcatacatat ataaatagag cagccgtgcc tgcgggaatt tctgctcgta
     1441 ttcgccgaac atcttctgca tgaccgtggc ttttccatgg acgttttcgt catgggacag
     1501 cggcagcagg tagtactcgt tggggaagta catcatggaa aatgtcagct tgtgataggt
     1561 ctcttcgtcc cggcgcaccc agggatactg ccggaaatag tccagagtgt cgttcatcca
     1621 gcccatgtcc cacttgtagt cgaatcccag tccgccgtat tccaccggac aggtcacgcc
     1681 ctcatagttg gtggaatcct cggcgatgag cattccctcc ggatgcagct ggtggagtcc
     1741 ctggttcatc cgcttgagga agttgatgct tgagggattc gtgccacggt tcgggtctcc
     1801 gccccagtag atgagccggc tcaccgcatc catccgcagt ccgtcaaagt ggaacacgtc
     1861 catccagtag ttggcgcagg actggagaaa actgcacacc tcgccacggg catggataaa
     1921 gttgcagctg ccccattcgc tgtagttgtc ctctttttcc gggtattcgt acagcgttgt
     1981 accgtcgtag tggttcagcg catagccgtc tacggcgaag tgtaccggca caaagtccag
     2041 gattacgccg atgcctgcct ggtggcaggt gtcgatgagc tgcatcagct ggtgcgccgt
     2101 gccgtaccgg gaggtgggac tgaaaaatcc ggtgttctga tagccccagg aacagtctgc
     2161 cggatgctcg ctcagcggca tgaactcgat gtagttgtag ccggcttttt tcacgtagtc
     2221 caccagtttc ggggcgatct cctcataggt gtaccagccg ttgggatcgt ccgggttggt
     2281 gcgccaggaa cccagatgca cctcgtagat attgacgggc ttgtgccggt ggtcgccacg
     2341 ctgtgccagc catttcttgt cgtgaaaggt gtagctggac aggtcacgaa tgatcgaggc
     2401 gttgttggga cgcagctcca tgccgaaccc gtaggggtcg cagtggtcga tgacgtcgcc
     2461 gtccggattc tggggagtga tgcggtattt atacatctgt cccggaacag cctccggaac
     2521 ggtcagctca tagacaccgc cgtcatgtac ctttttcatc ggcatcggct cccactcgcc
     2581 gccttcccag atcagatcga cggctgcggc atttggcgca agggtgcgga acacaacgcc
     2641 ctcttctgtg acgtgtgcgc cgaaataagt atgggcatca aaggaattgc ccagaaaaaa
     2701 ctgatgaata tccatggggt ttgttcactc ctctatgttg tttcgcaatt gtacgtaaaa
     2761 ttttacgcaa atacatagga ttctagtact attatactaa aactgcatac agtattgcaa
     2821 gcaccaaatc catatgcagt gttgcattat agacaaaagt ttattttctg tagggcatcc
     2881 gaacactttt tgagaaaata cgaaaaaatt tttggaaacc cattgcattt gaacccgaaa
     2941 tgcggtataa taaatgtagc ggacattctg aaacaggaat atctgttgca ccaaattgaa
     3001 ttaggaggca cttttttatg ttagtatcgg caaaggatat gatggacaag gcaagagacg
     3061 gcaagtatgc agtcggtcag ttcaacatca acaatctgga atggacaaag gcaatcctga
     3121 acacagcaca gaagctgaac tccccggtta tcctgggcgt ttccggcggc gctggcaagt
     3181 atatgaccgg tttcaaggtt gttgcagata tggtaaaggc tatggacgaa tctctgggta
     3241 tcacagttcc ggttgcactg cacctggacc acggtactta cgatcagtgc tatgactgca
     3301 tcaaggcagg ctttacttcc atcatgttcg atggttctca cttcccgatc gaagaaaacc
     3361 tggcaaagac caaggaactg gttgctgttg cgcataagct gggtctgtcc atcgaggcag
     3421 aggtcggcgc aatcggcggc gaagaagacg gcgttatcgg caagggcgaa tgcgctgatc
     3481 cgcaggaatg caagaccatc gctgatctgg gcgtagacat cctggctgcc ggcatcggca
     3541 acatccacgg tgtatatccg gcaaactggg aaggtctgag cttcgagaca ctggaagctg
     3601 ttaagaaggc tgtcggcgct atgccgctgg tactgcacgg cggtaccggt atcccggacg
     3661 accagatcac aaaggcaatc tccctgggcg ttgcaaagat caacgtaaac acagagtgcc
     3721 agctggtatt tgctgaggct accagaaagt acatcgaaga aggcaaggat caggtaggca
     3781 agggctttga cccgagaaag ctgctcctgc cgggctacga ggcaatcatg gctaaggtag
     3841 aagagaagat gaacctgttt ggttctgtca acaaggcata agttcttctg acacttgcag
     3901 agaaaggaat gtgttcccga tgaaaaagac gctacaggtt atactctctg tactggagat
     3961 cgtcatgtac accacaggca tcctggtttc ggcaaagacc ttggtggaac tggcacattc
     4021 tgacgaatgc gaaaacaaca cagaagaggc gtaaccctct gaaagaaacc ggtttgcatt
     4081 gaaacggatg cagaccggtt ttttgtgtgc agcagagaaa aaacaaatat gatgcgaaaa
     4141 acgtaaattt ttttggaaaa aaacgtactt ttttcttcaa aacccttgat tttttcgtcc
     4201 ggcagtgtta taataatgat aatcatgcag caaaagaccg gaaccccacc ggtctttcgt
     4261 ttatgttggc acttttttga ttgcagagcg cagtgaaaaa agaatgcaat acgctggaaa
     4321 aaggagcaga tgccatgaaa atgaagaagt tcggcgggac agaaaaagtg gtctatgacc
     4381 tggtgaagcc catcgccgat gaacagggct attccatctg ggatgtgagc tttgaaaagg
     4441 agggtgcata ctggtacctg cgtgttttca tcgaccatcc ggacgggatc gacatcgagg
     4501 actgtgaaaa gatgacacgt ccggtcaatg cggcggtaga tgccgcagat cccatcgcac
     4561 aggcatacgt gctggaggtc ggctcggcag gactggaacg ggaactgaac cagccctggc
     4621 actttgaaga aactctgggc atggaggtcc gtgccagagc catccgtccc atcgagggcg
     4681 aaaaggaatg gatcggcgta ttgacagcgt atgatacaga acaaatcacc atacagccgg
     4741 aggatcgtga ggcgcccata actctggcgc tgcgggatct gtcgtatgtg cggcggtatg
     4801 taacagtaga attttaatca acggaggcat agtgaagaaa atggacaaca gtttttttga
     4861 ggcgctttcc tcactgggaa gcgaagcaaa tgtggatact gctctgctgg tggaaaaagt
     4921 aaagtctgcc atgctgaaag cagccagaan nnnnnnnnna aggcgtatcc ggagtgtgaa
     4981 gaaaacatca gcgtagagat cgacccggcg accaagacat ttgagatgta cctgcgccag
     5041 accgtggtag acgatgagcc gatcgacgcc aatgaggtca acatcgacgt ggcacgcacc
     5101 atcgacccca acgcctgtgt gggcggattt attgaaagac ggctggacat cgccaagttc
     5161 ggcagagcag ccgcacagtc tgcaaagcag tccatcaagg gtgatctgcg tgagatcaac
     5221 cgggaacgca tcctgggcga gttcgagtcc ctggaaaacg actgcatcac ctgtgaggtg
     5281 tctcaggtcg agaccggcgg cacagcgacc ctgctgtatc acaagaccga actgtatctg
     5341 ttccgcaacg agcagatccc gggcgaagtg ctgaaagagg gacagcgtgt caaggtttac
     5401 atcaccacca tcgccaacaa gcagaaaaag cccatcatca agatttcacg tgtccgcaag
     5461 gaactggtaa agcgcctgtt tgagctggaa atcccggaga tttacgacgg cacggtggag
     5521 atcaagtcca tttcccgtga ggcaggctcc cgtacaaagg tggctgtctg gtcgaacgac
     5581 cccaatgtag atgccatcgg cgcctgcatc ggtccgaagc gctcccgtat ctctgccatt
     5641 gtggctgagc tgaacggcga gaagatcgac attattcctt acagcgaaaa gccggaggaa
     5701 ttcatcgcca aggcactggc accggcaaat gtgctggatg ttcagatcct ggacgaagaa
     5761 gagcgtacct gcaccgttaa ggtgccgaac aaccagcttt ctctggcaat cggcaacaag
     5821 ggtcagaacg caaagctggc agcaaagctg accggctata agatcgacat caagccggaa
     5881 tttgatgtgc tgcatcagaa cgatgctccg gcagaggaaa aggcggagac tccggtggaa
     5941 cagccgacag atgcaccggc agagacagag gcagcagcac cggaggcgaa cagcgttgaa
     6001 gagtaaaaaa attcccatgc ggctttgcgt gggatgcggc accatgcagc cgaaaaaaga
     6061 tctcatccgg gtgctgaaaa atgcggacgg tgaaatgaaa ctggatttta caggaaaaca
     6121 gtccggacgt ggcgcatatc tatgtaa
//
LOCUS       DAWENO010000122         6099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5102_length_6099_cov_11.356717, whole genome shotgun sequence.
ACCESSION   DAWENO010000122 DAWENO010000000
VERSION     DAWENO010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5102_length_6099_cov_11.356717"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1377 a         1619 c         1561 g         1542 t
ORIGIN      
        1 ctcgcctgtc cggtctcgcc ctcttccagg gtgacgttcc acagatacgc actggatgac
       61 gcatcggtgg taaagcccgg gtctacaacc accatatttt tctgggtata gcacgccagg
      121 ctatccgact tgtcctgtac cgtgactgta aaggaagaat catactcgcc ggtgctgata
      181 ttgtattcgc agctgtccag caggaactca tacgttccgt catcgtacag cgtggtgtcg
      241 tagtacagaa tgataccgtc cggcgtatcc ttttcaattt tcacctgcac ctcacgctcg
      301 ccggaaactg tcatggtgag cgtgccggta cgatcctgca aatagccgct gggcgaaatg
      361 agatagctgt cgtctgcgaa tgcgctccct gccggaagtg ccagtgcagc cataacggct
      421 gcggtcagtg ctgccatgca gcgttttcct gttctcttca tctgtgaaac ctccgcttaa
      481 tactgaacat attccgttgc gtcactgtat ggatccagga agtgcatcca actgccgttt
      541 ttgccctcgt ttccggtctc catcacgtat gttttgccgt tgatatgcac ctcgcaccag
      601 aagtgctgat tgccgacgcc gccgacccag ccctgtacaa ggttgacatc atagcccagg
      661 tatgccatca tggatgcgat cgccgcatta tactggatgc actgtccctt gcggtaggtg
      721 aagatggcgt ccacatagga cttgttggtg atctgcgccc aggcgccgtt ctgcacgtag
      781 gcatattcca cattggtgtg gatccagttc agcgttgtcc agagctgctg ttcccgtgtc
      841 atgcccttct tgaagttctt gttgataaag ttttgcagga tctgcttgtc cttattggaa
      901 atgtacgttg tccactggct gtggttcttg gtttggcggt tatagaccac aaagctgtag
      961 tgaggtttca gcgtcgcctg gttcagccga tagcatacca ccgagatacc cgaaccgtcc
     1021 gactggagat aatagttgcc ccagtgttcg ctgtcgtagt agcagaacac ttccttattc
     1081 ttctgcattg ccccgtcata gccgaagtaa tacaacttgc cgccgatcat ctgccactcg
     1141 gtctgcatca cgccgctggc gttgaaataa tagttctgcc cgttcagttt ctgccagctt
     1201 ttcaccatct ttccctggtc gttcaggtag taggtatcgc cgttcagatt gagaaagccg
     1261 tagtgcatcg caccgtctgc gtcaaagtag taccgtgcgc cggaaatgtc ctggaatccg
     1321 gtgcgcatca cgccgtccga tgcaaaataa tacttttcgc cgccgatatt ctgccagccg
     1381 gtctgcatgg cgtaattgct gtcgaaataa tagacctttc cgctgtccgc cgtcacccaa
     1441 ccagaaagca cttcgccgtc ctcgtcataa taagaggttg tcttgtccgc aaaggtgcag
     1501 agtcccagct gctgctgacc gtaggtgtcg tccagcagat agcgcttgcc gtccttctgg
     1561 aactctccgg tctgcttgcc ggtctcttcg tcggcatagt aacggttgtc gcagtaggtc
     1621 agccagccgc tgaccatctc cccggtctcc gggtcataat agcggcgcac cccgttgacg
     1681 gtacgccagc cggttttctg cacgccggtg taatcgaaca gatagtacac gccgtcgatc
     1741 tcctgctccc cggtcacagc gtttccggat tcgtcgaaat accggcgcac gccgtattcg
     1801 ttctgccagc cgggttcgcc ggtcatggaa gtcaccgccg ttgccaccgg gacatccggc
     1861 gtttccagct ctgccgcaga tgcagatacg gtactgccca gcagaatgcc gccgcccagc
     1921 actgccccga gggctgccag gatgcagatt gtttttcgtc tcatagatca tttccccttt
     1981 cccgttttcg gcactgcaaa cagcgccggg aatttaccat gcagtgtgcc atttttgccc
     2041 acgctcctat tagtttacca aattttcacg aatttgtcaa gagtgaggtg cagtgtgaaa
     2101 gaaaatcaat ctttcaaaaa gtctgccttg aaaagctgtc tgtaggggcg aacaatgttc
     2161 gcccgggtcg actcctccgt ccttcggaca cttcctctat gagaggaggc atatattagg
     2221 tttccctttc ggacaatgca ctcctttcca tttgacaaaa cacccgtcat gtactataat
     2281 aggaacaaat ctatcagccg ggagaactgc ggcgtttgct gtcattttcc gggaaaagtc
     2341 aggagtgacc cttttatgta tcaacttgcc attttcgacc tggacggcac actggtcaat
     2401 tccatcgccg acctgggcgg cacagccaac cgcctgctgg aacagcgtgg actgcccacc
     2461 catcccctgg atgcgtacta tcgctttgtg gggaacggcg tccacaagct ctgcgagcgc
     2521 tgcctgccgg cagacacacc ggcggacgag gtggaacggt tcttcgccgc attcaacgag
     2581 gactacagcc gccactgcct ggacaagact gcaccctatg ccggcatccc cgaagtgctg
     2641 gaacagctgc gcagcgccgg ggtacacatc gcagtagcat ccaacaagac ccagcccttt
     2701 gtggaaaaga tcgtgaagca ttatttcggt gaaaacgggt tcgatcagat cctgggcagc
     2761 agcgacagcc gtcccaaaaa gccttcgccg gagatcatct ctgtgatttt ggaaaacgca
     2821 ggcatgacac cggaacaggc ggtcatgatc ggcgattcca acgtggacat ccgcaccgcc
     2881 aaaaatgcca gcgtacattc catcggctgc acctggggct ttcggggctg ggacgaactg
     2941 ggcgaggcag gcgcagacga tctggcggaa aagccggcag atctgctgaa attcacgcta
     3001 atgtgacagg atcttccttt attttttctg gtatttcggg aaaaattgaa atttgcttga
     3061 caatcccgtc ctttttggat ataatgggtt ctgtgacaaa tgcaggacgg ttcggcagga
     3121 acggtgcagg gatgttccgg cataggtcac ctctgggcag ggacagcatc cggttttgtg
     3181 cgtttttgca aaagagcgcc tacagaaata ttttgatttt tttgttctac aaacggaacg
     3241 ctttctgctg cacaggataa aattttcttt ttcagctctg tctcccgtga gacagagcct
     3301 ttttcttgcc gctttttaat ctgtgtttca aacggattca gaatttgcaa tcgaaatttg
     3361 aaattcggat atctttcttt ttttcggttt ctgcgatttc gatatcaaga aaatgcaaaa
     3421 gttcgtcaat tttgcattga aaaacatagc cgaatggtgt ataatagatg ttgttcgaag
     3481 tgcgtgtaca ggtgtttgaa gcgaatgcac atacatcaca tacattttga gtttggacag
     3541 cgtgcggata cgggttttct gacagaacgt cccgtatcct tgacagaatg ttttgctaca
     3601 ttttaatcca acatttttat ccaacaggag gagattccca tggcatttac atttaagaac
     3661 gcttatcttc agggcgtata cgacagcgtc gtaaagcgca acggcaacga gccggaattc
     3721 ctgcaggcag tcggcgaagt tctgatgtct ctggagccgg tcgtagcaaa ggatccgtct
     3781 tatgagacaa acggcgtaat cgaccgcatc gtagaaccgg agcgcatgat ccagttccgt
     3841 gtttcctggg tagacgacaa cggcaaggtt caggtaaacc gtggtttccg ctgccagttc
     3901 aactctgcaa tcggaccgta caagggcggt ctgcgtctgc acccgtctgt atgcgcatct
     3961 gttattaagt tcctgggctt tgagcagatc ttcaagaaca gcctgaccac actgccgatg
     4021 ggcggcggca agggcggttc tgacttcgac ccgaagggca agtctgacaa cgagatcatg
     4081 cgtttctgcc agagctttat gactgagctg tccaagcaca tcggcgctga cactgacgtt
     4141 ccggctggcg acatcggtac cggcgcacgt gagatcggct atatgttcgg tcagtacaag
     4201 agactgcgca acgagttcac cggcgttctg accggtaagg gtctgtccta cggcggttct
     4261 ctggcaagaa cagaggctac cggttacggt ctgctgtact tcactgaaga gatgatgagc
     4321 tgcatgaaga aggacaccat caagggcaag actgttgtta tttccggttc cggcaacgtt
     4381 gcaatctatg caacggaaaa ggcacaggaa atgggcgcaa aggttgttgc actgtctgac
     4441 tccaacggct acatctacga tccgaacggc atcaagctgg atgttgtaaa gcagatcaag
     4501 gaagtagaac gtggacgcat caaggaatat gtaaaccgtg tagacggcgc tacctatacc
     4561 gaaggctgca agggcatctg gaccatcaag tgcgatgttg ctctgccgtg tgcaacccag
     4621 aacgagatcg acggcgagtc tgctgacatc ctgctgaaga atggcgttca ggtcgtagca
     4681 gaaggtgcaa atatgccttc tactccggaa gcaatcgaca agttcctggc tgcaggcatc
     4741 ctgtttggac cggcaaaggc agcaaacgca ggcggcgtag ctacctctgg tctggaaatg
     4801 tctcagaaca gcgagcgtct gtcctggaca ttcgaagaag ttgatgctaa gctgaagcag
     4861 atcatgatca acatcttcca caacgcatac gatcgttcca aggagtacga cgtagaaggc
     4921 aatctggttg tcggcgcaaa catcgcaggc ttcctgaagg ttgctgacgc tatgaagtgg
     4981 cagggcgttg catattgatc gaaggctttc ggttgatata atttggtaat tctctcaatc
     5041 ttataaaaag cagcctgtct ccggggtttg tcctggaggc aggctgtttt tgttttcgat
     5101 gactcctcca ccgcctcttt cagaggaggc taattcgcag acacaacaca atctgtatac
     5161 acaaaaaggc gtggaacttt tcacagtccc acgccttttc tatttgcaaa acgctgttca
     5221 atcctcttcc agctcaaacg gctcttcccg gttggaaaac gccgcatcca aaatcttgcc
     5281 gtaaaatcct gccgggttca gcatgatctg ttcgcccaga tacttcgcct gggaataatc
     5341 cggggcgtag agtttcatat ccagcaggtc gtccccgatg tccagacagc ggacatggac
     5401 gtaatagccc tttttcagcg gaatgtaatt gatcttgact tcgctctccc gtttgatgcg
     5461 tgcaaaatag tggaacgccg ccgagaccag cttgtcccgg tacgcctttc ccacatagtt
     5521 gcgcagttcc tttgccgtgc ggatgccgtt ttccgtcagc acatagtact ccgtctcgtc
     5581 atccttgcgg cgtagctcta ccgagtcgtg ttccgtcaga taagcgatgg aatcctggta
     5641 ggcaaaataa ttgataatgt cactcccgac tgcaatgtca tacaaatgat tcacacgcac
     5701 cggcttgtcg atcttataca gcagataaca aatcaaaatg ttcgccgtgt ggggatccat
     5761 aatgggcggt gtcgccattt tggacatttt cagcatattt tcttcctgca caacctgtca
     5821 cctcaaatca cagaaaaaag ctttttctca aaaattcgga taatccatca tatgggacag
     5881 cagcgcaatg ttcactgccg cctcaacgca cggcacagca cgtggcacga tgcacgggtc
     5941 atgtctgccg tgaatctgga gcgtctcgtt cttctttgcc cggaagtcca cggtctcctg
     6001 gggctttgcg atggaggcgg tgggcttgat ggctacattc agcgtgatgg gcattcccga
     6061 agagatgccg cccagaatgc cgccgtggtt gttggtctt
//
LOCUS       DAWENO010000123         6054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5148_length_6054_cov_8.722954, whole genome shotgun sequence.
ACCESSION   DAWENO010000123 DAWENO010000000
VERSION     DAWENO010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5148_length_6054_cov_8.722954"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    993..1002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1682 a         1246 c         1574 g         1542 t
ORIGIN      
        1 taaaatcaag ggattccggt cagggagcta ccgagtagcg accacgattt cgagtgcagc
       61 tcgttatgac cacttcgata actctccgta agattaatct gatcatatca gaacaaaata
      121 tattatagca cattccgata aaaaaagcaa gtggttttga aaatttgcct ttttgcacaa
      181 atgaaaggcg ctacttttag atgaatttat ttgccttgtc gttttttgac ttgctttgcg
      241 gaaatacttg acttttttat cgaaatcacg tataataaag tgaaacgcat tgtatcggca
      301 gtaccatgca aatcttgtgt gcagctgccg gtgaatcgag aaaatgtttc ttttcgaaag
      361 gaaaggtctt aaaagcatga aaaacttttt cgcaagttta tcacatcgta cgaaaattac
      421 actaattacc tgtgccagct ttattgtgct gacaatgctg atcttgattt ttttgatgct
      481 ttgtccgata caggaaagca gttctgcaaa tcatgtaaat gattcgttag ttgtcacaac
      541 agcagtttca gagggaacac tggatacggc agagacaacg acggctgtat ctaccacaaa
      601 gtttaatcga aaaacaacgg acgcaaagcg ctcaacgacc agaacagaaa ctactgtgaa
      661 gtcatatcag acacaggctg tgtatgacga taacacatat gcagatgatg atggctacaa
      721 caatgatgat aattacgagg attatcatcc ggttgtcaca acgaaaaaat attacagcaa
      781 tagcacaaca aagtatacaa cagctgctcc ggcggttaca cagccgccca caccggcagc
      841 tacacaggct ccggcagtga cagcagcgcc tgcaccgagt gaaacaattc cggtgccgga
      901 tgacacgatg tacgattgag gcgttgttcc tggaaaacgg gagaatgttg tgtatgttca
      961 cttgaaaaaa cataggttga gagaaaccgg acnnnnnnnn nngtgttgaa tgggggaaat
     1021 atgaaaacag aggtatgggg agttcgtctg gctcggctgc tgctgttttg gtttgcagga
     1081 aatttgacag ccttttttgc tattgtgcat tttgcagtcg gctggaaaat actgattggc
     1141 attctgacgg gaattgcttt tttgttcttt cagttttttc atccgcatac agtagcaggg
     1201 gaaaagaaac tggcatcttt ggagcatggc tgtaatttac tccgaaccgg agcagtctgg
     1261 cttgttctgg aatgcgtcac agtgggcatt ttgatttgga gtcatgcgct gttctgggcg
     1321 ttggaattgg tgaacctggg tgtgtttgcg ctactttgct gggcaatcgt gttccaagga
     1381 cttttacata ttgcactcca ttcttcgcag gtaaagctgc cgtggcacat tgccttgttt
     1441 ttcctgtggt ggatgcctgt actgaacttg tttctcattg tacatattta tcgtactgca
     1501 aaacatgaac tgcatctgga atgcgcaaaa gcagaatgtg atatcgttcg gaaagaatct
     1561 gagatctgca aaaccagata cccgatactc ctggttcatg gtatcttttt ccgggattgg
     1621 cagcttttta attactgggg aagaattcct gcggaactgc aaaaaaatgg cgcagtgatc
     1681 ttctatggca agcagcagtc tgcacagtcg atttccgaaa gtgccagaga actggcggct
     1741 cagatcaaag cgatatgtac ggaaaccggt gcagaaaaag tcaacatcat tgcacattcc
     1801 aaaggcggtt tggactgccg atgtgcactg caggactatg gagtctcaca gtatgtggca
     1861 tcactgacaa cgatcaacac accccatcat ggatgcgcct ttgtagatga cttactgcga
     1921 aaagttccgg ataaaacggc gcagtggatt gctgaccgct ataataagct ctttctcaaa
     1981 ttaggagacg atcatccgga ttttctggca ggtgtccggg aactgacaga agaaagctgc
     2041 cggaagttcc atgtggcgca tccttgcctg ccaaatgtgt attatcagtg cgtaatgtca
     2101 aagatgcaca gtgcattttc cgcaccgttt ccactttggc tgggatatct tctgaacaag
     2161 cggtgcgccg gcgaaaatga tggacttgtg ccggtctctt ctgcgaaaat ggaaaatgtt
     2221 ccgctgctta tggttccgga tgcaaagcgt cgtggtattt ctcatgggga tatgatcgac
     2281 ttgaatcgtg agaatattcc gggatttgat gtccgggaat ggtatgttca gctggtgcag
     2341 cggctgaaac gaaaagggtt ctgaggtgat gacaatgaaa acaacatacg atgcaaatat
     2401 tttgacctat tgtcagtggc gggatgatct gacaatggag caatcgccgt tttgtcatgt
     2461 tgacaatttg attttctgct gtctggcgta cttgaattgg gacggtatcg cagagggatt
     2521 tactgtctcg gaatcagtgt ctcttcggga tgctgcgaaa ctctgggaaa gccagccggc
     2581 gaaggaacag aaactgcgtg tggaaacaga ccgggaactg ctgcggcaaa ctgctgccag
     2641 ccggcgtttc ggagatattc gtctgtttcg gtatacagaa caattcagtg atacattgca
     2701 gcagcaattt tcagcaacag cattcttgtt ggacgcaaat acgatctatg ttgcatttcg
     2761 tggtacagat gatacgctga ctggctggcg ggaagatttc aacctgtcat ttcttccgaa
     2821 ggtaccgtcc caggaatcgg ctgctacata tctgagaagt attatgacac tgggtttccg
     2881 gaatgtatat gtgggcggac attccaaagg cggaaacctg gcggtgtatg cggcggcgca
     2941 gatgacaccg gaaaatgctg cccatatccg tggcgtttat aataatgacg gaccgggttt
     3001 tgtaacggac ttgtttgatg cgccggagtt tcagccgctg caaaagcgag tgcatacctt
     3061 tgtgccgcag gcatccgttg tagggatgct gttggaacac gatgaggcgt atcaagtggt
     3121 ttacagcaca cagaaaggca tcatgcagca cgacccgtat tcctggtgcg tgattcgaaa
     3181 tgactggtat tacctggacg atacgacaac gctcagcaag atcatggatt cctctatgcg
     3241 gcagtggatt ctgggaatgc agccggaaca gcgtgaaaaa ctgacagatg caatttttca
     3301 tgtgctgcaa agcgagacaa atgccaaaac agtgcaggat ctggtagacg gcggtgcagg
     3361 aacactttct gcagtgctgc atgcctggtc ggatatgccg ccggaaacca gacggtttat
     3421 gcaaaaaatg ctgggtgaac tgctgcgtat cataggacag cttgcagcgg caaagcgaag
     3481 gtctgaaacg ctcgccggac gaataggtgc cgatgaggac aatggaaagc tgatgtgata
     3541 tgagaaagcg tattttggca tatcgacgga gaatagatgc ccttttggca cagcatccgg
     3601 aaaatacaga ctggaatcgg attttacaag atcatctgcg gcaaatcgcc ttttttcagc
     3661 acgaacgtct ggtacatctg atcgttacaa tgacttttgc agttttgaca atgctttcgt
     3721 ttggcatcgt cctgcttaca cagcttattc agctgactgt gcttactgcg gcattgttgg
     3781 ttctgttgat cccgtatatt tttcattact atctgctgga aaacgaagtc cagaaaatgt
     3841 atgcgcagta tgatgaaatc tgggagaact gcaatatcat attacaggaa aagtgtcttg
     3901 ggaagtcatc caaaaagtaa atcgcatttt tcacaaaaac ggaactgaaa tcacaaaaaa
     3961 actgttgact ttttcttgca aatcgcttat aatgaaaata atcgaatgtg tgttttccaa
     4021 ggagcataag ctgcgttttc gccatatggg aatggattcc ggcgaaagca cagagggagc
     4081 ttcgtgtttt ggaaaggaaa ggaattcaat cagatgaagc attttgcaaa aggatcaaaa
     4141 cgattgctgt cggttctgct gagcagtgcg atggtagtca gcagctgcgg ctcagtttcg
     4201 gctggtgcgg cagagaagaa ggaagcgaac tttgcacggg cgctgcagta ttccatttac
     4261 ttttatgatg gaaatatgtg cggtacagag gtgcaggaaa acaaccggta cagttggcgt
     4321 ggagactgtc atacatatga tgcacaagtt gcaatgaaca gtacagctac caatctgagc
     4381 gcagattttc tcacgaagta taaagatgtg cttgatccgg acggcgacgg ctatatagat
     4441 gttgccggcg gattccatga tgcaggtgac catgtgaagt ttggtatgcc ggaaaactat
     4501 tccgccgcca ccctgggatg gggctattat gaattccggg atgcgtatga aaagactggt
     4561 caaaacgatc acattgagac gatcctccgc tatttcaacg attatttgat gaagtgtaca
     4621 ttcctggaca gcaatggcga cgtgattgcg cattgttacc aggtcggcga cggcgatatc
     4681 gaccacgctt actggaatgc accggagctg gacgaaatgg atcgtccggc gtatttcctg
     4741 acagcagaca agccgcagac agactatgtt gcatctgctg ctgcatctct ggcaatcaac
     4801 tatctgaact ttaaggatac cgacccggag tatgcgcaga agtcactgga ctatgcaact
     4861 gcattgtaca agtttgcaga gacgcatgac aaggaactca gcgacaacgg agacggtccg
     4921 aagtcttatt acaattcgtc aaaatgggaa gatgattact gctgggcatc tgcctggatg
     4981 tacaagatca caggagacca tcactatctg gaacaaattt tcccgtacta tgactattat
     5041 gcagcaccct gctatgtata ctgctggaac gatgtctggg gcggggtaca gtgtattctg
     5101 ggcgagatca ccagcgaaca gtacccgaac ttcattgacg aatataaaga ggcagccgga
     5161 aagagtcctt atgaagagat gaactgctgg ggctctgtgg aagaagcact gaataagtat
     5221 atgaccggcg gcgtcggaac gatcacaccg gctggctact tctggctgaa cacctggggt
     5281 tctgcaagat ataatgcagc ggcacagatg atggcattgg tatacgacaa gtataacaac
     5341 aatggcaagc ccggaaagta cagtgaatgg gcaaagggac agatggaata tctgctgggt
     5401 gacaatccga tgaatcgtgc atatgaagtc gggtatgatg aaaccgcagc aaaattccca
     5461 catcaccgtg cggcatctgg tttgacgaag tgcgaggaca ccgatgaaca gaaacatgtc
     5521 ctctatggtg cattggtcgg cggtccggat gcacaggata agcacaacga cattacagct
     5581 gactggatct acaacgaagt gacgatcgac tataacgctg catttgtcgg agcgtgtgcc
     5641 ggactgtatg actactatgg aacagacgcc atggaaatca caccgaattt tccgccggaa
     5701 gacaagaatt ccggaaatga gggcggcaat gatttctggg tagatgctta tgcagtagat
     5761 gacattcaga caagcggagc aggcgtgacc aagcttgcga tccagatgcg caccaactcg
     5821 attacgccaa aaacagatct ttcgatgcgg tattatttca gtatcgctga actgaaggat
     5881 aagtcaaata tcagtctggt aaaaggacag gaactgtatg accaggcgag tgtagaggcg
     5941 gctccggcag atggcgtgat ttccggaccg tatcagtttg atgcgtctta tgatccggat
     6001 atctactatg ttgaagtaaa atgggatggc tataagattg caaactccaa taag
//
LOCUS       DAWENO010000124         6031 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5171_length_6031_cov_11.190930, whole genome shotgun sequence.
ACCESSION   DAWENO010000124 DAWENO010000000
VERSION     DAWENO010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5171_length_6031_cov_11.190930"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1422 a         1502 c         1739 g         1368 t
ORIGIN      
        1 tttgattgga aatcagacct tgtttcccac gggcaaaaat gtgtttgctc agatgggaaa
       61 cacattggaa aaatagttcg agaggagaac aaatgcagca aagacaaaag aagaaacagg
      121 cgatgcgccg gtttgcagca tctctgaccg cagcagtgct ggcggcggca tctctgccgg
      181 tgtgcgccgt atcatccgag ccgtacaaca cctggacggg atacgtgctg cgtgaccctg
      241 ccgggcgcta tctcagcgtt tccggcggca cagagggcga gggcgtacag gtcggctttt
      301 acgaggcaga tggcgtagca ccctacaaca cgtggtactt cacggaaacg gactggggca
      361 ttaccatcaa atctgccctg tcccagggag agtactactt aacagagaat accaaggaaa
      421 acaggctgga gctttccacc tctgccggga gttttgaact gggcgatgac ggctatctgc
      481 gtggattccg cagcggcgtg gagtccccgg tctatgcaca ggtgacaccg gaggctgtga
      541 caaacctgca catgggcgac tggaacggcg acggtgttgt agacggcaca gacctggtgc
      601 agctgcggca gaccgtacag ctggattcag acgactcgtc tgtcagcttt gtccggcagg
      661 cagtgggcga taccaacgga gacggcgtat tagacggagc agatgtgcag ctgctccagc
      721 agtatctgct gggcgagtcg gtttcgcttg cggcagtttc catccccacc tgtaccatgg
      781 tgccgccggc aatggaacag ccggaaccgg gaacgacaga accggtcact accacaatga
      841 caacaaccga aaccacgaca acaactgaaa ccacacagac atacagggat accgcagcag
      901 tgactacgga gaccaccact gcaccgccgc agcaggatct caccatggcg gactatccgg
      961 cagagtacct ggatgcgtcc gactggatct ggaacaaccg tatcaatacg gaaggctctg
     1021 ccaaggactg ggcgaccatc tatgaccaga tcgttgccgg aaacggcacg ctgcactata
     1081 ttctgatgtg gcagtcctat gagaagatca ctctggaaca gcggcagaaa ctgcctcaga
     1141 tgttggagga tgcggtcaac cagtggacgg atcatctgaa aggctgggac ggctggaagt
     1201 ttgaccacgt caaggtagaa gttgtgggct atgctgtgct ggataaaaac tgcctgctgg
     1261 acctccagcc ggatgaagtg gtctatacgg ataccacaag ctcgtggctg cgggacgata
     1321 tgatttccag cggcatgggc gattccagtg tgcctgcaat tcagccggct gagccgactg
     1381 acatctcccg gtattcccat tgggcagata agaactggac gtataacggc agctatgaaa
     1441 accggtatga tatgtacctc cacggcatca ccggcatgat caacatgggc ggctatggct
     1501 atcactacgg gcagattctg tccgaccagt ctgtgctggg actgatcgac ggcaccacca
     1561 gccagcacat tctgctgcac gagatggggc acggtttcgg gctgcccgac tattacggcg
     1621 gcgagggcga gtctgacgga ttcccgcccg gcggattccc cggcggcgaa aactccatta
     1681 tgatggctgg ttcttcgcag aagatcactg actttgacgg ctggttcttc cggtatctgt
     1741 ggagcaagct caagtcagag gacggcagat ttcaataaga gagtgatatc agggcatttg
     1801 tacgcacaga tgctgaacag agaggaaatt gggtatgaag aaacagagaa tcctggcatc
     1861 actgacagcg gcggcgcttg ctgcggcgtc tctgccggcg gttgcagcct catcggtgga
     1921 atacaacacc tggacgggat atgtgctgcg gaatgcggca gggaactgcc tcagcgttct
     1981 cggcggctca gagggtgagg gcgcacaggt cgatttttac ttggcagacg gcgcagcgcc
     2041 ctataatacg tggtatttca ccgagacaga caccggcatc accgtcaagt ctgccctttc
     2101 gcagggcgag tattacctga aagagatgga tggtatgctg gcgctgtcca aaacagcagg
     2161 ggaattcacc ctgtcagagg acggcacact gctgggcgca tccggcaagg catacgacat
     2221 tgtggtgccg gaaccggtga ccaatctccg catgggagac tggaatcatg acggtgcagt
     2281 ggacggcatg gacttgacac tgctgcggca ggcagtgcag gatcacacgg cagactttgc
     2341 gcagcaggcg gtaggtgaca ccaacgggga cggtgtgttg aatggggcag atatacagct
     2401 gctccagcag tatctgctgg gcgaggatgt gaccatggca gaggtacacg tgccggaatg
     2461 cagcattgtg ccgatgccga atccgccgga gaccacagaa cctgccgtca cggaaaccac
     2521 tacgaccaca gaaacaacga ccacaacgat gggaacggct gctgccacag agaccacggc
     2581 aaccacagaa acgaccatag tgacaactac cacagaaaca ccccggcagg atctgacgat
     2641 ggcagacttt ccgtcggatt atacctatgc ggcagactgg atctggaaca accgtattgc
     2701 ggcggaaaag tctacggaac gccggaacac cattttcgac cagatcgacg ccggaaacgg
     2761 tacgatcaac tatgtggtgc ggtggcagtc ctaccgcaca ctgaccctgg aacagcggca
     2821 gcagtttgaa aagctggtga gcgactgcat caacggctgg aacgactggc tcaagggctg
     2881 ggacggctgg aagtatgacc acgtggacgt gaaaattgtg ggctgggcag tgctggacaa
     2941 atctgttttg ctggatttgc agccggatga ggtcgtttat gacaatctca tctccgacta
     3001 tgacagcacc tatgacacat ccaacggcgt ggacactatc ccggataagc tgcccagtgc
     3061 gccgtctgag atttcccggt tcgaccactt cttcgacagc agttacagct atcccggcgg
     3121 tctggacaaa cgctttgata tgtacctgtg gggcacgcag ggattcccga acatcggcgg
     3181 ctgcggcggt gactggggac agcggctttc ggatacggcg tacctcaata tgctggacgg
     3241 ctccggtatc cacgtgctgg aacacgagat cggtcacggc tttggcatga cggatttcta
     3301 cggcggcgag ggcgaatcca acggattccc gcccggagga ttccccggcg gtgaaaattc
     3361 tctgatgatg gctggctctg ccatgaagat cacggatttt gacggctgga tgctgcgcta
     3421 tatgtggagc aagatcaagg acgaggacgg acggttctcg aagtaatacc agaaaaaaaa
     3481 cgccttctgc acgttggatg caggaggcgt tttgtgctgc cgggatgatt gcaattttct
     3541 tcgaagtgtg gtatactttt acagttgact gtgattttca gacagaaggg agtatgatcg
     3601 tatgaagtgg aaagcatttc gggcggcatt tccgcacaca ctgcccattt ttgccggatt
     3661 ctggtttctg gggcttgcct atggcatcta tatgcacgtc gccggctttt catttgtcta
     3721 tccgatgctg atgagtcttt tgattttcgg cggctcactg gaatttgttg ctgtggagat
     3781 gctgctcagt ccgtttgccc cggtacaggt gtttatcatg acactgctga tacaggcacg
     3841 ccacctgttt tacggcatat ccatgctgga caagtataaa ggcatggggt ggaaaaaggg
     3901 atatctgatt ttcggaatgt gtgatgagac cttttccatc aactatacgg cagagatccc
     3961 ggacggcgtg gacaagggat ggttcttttt ctttgtgacc ctgctgaacc atttttactg
     4021 gttttccggg gcgaccatcg gcggacttgt tggctcgctt ttgaaatttg ataccacagg
     4081 catcagcttt gtgatgaccg ccatgtttgt ggtgattttt ctggaacagt ggctgaaagg
     4141 aaagcaccat gccgcctcat ggatcggtct gctggtatct gtggtctgcc tggtctgctt
     4201 tggcgcagat tcttttttga ttcccaccat ggcggcgatt cttctgacat tgcttttgtg
     4261 ccgcagaaaa ctggaacgaa cggagaaaaa atcatgacac tgacccaaca gatcattaca
     4321 ataggcttgt gtatccttgg aacgatgctg acccgttttc tgccgttttt gattttccgg
     4381 gaaaaccgaa aaacgccgga agtgatacaa tatatagggg aatatctgcc ggctgcggtt
     4441 ttcgggatgc tggtggtcta ttgcctgcgg aatgtcagca tcgtctccgg cagtcacgga
     4501 ctcccggagc tgatcgccat tctggtgacc tgcggactgc acctctggaa acggcagatt
     4561 ctgctctcca ttgccggcgg cacggtgtgc tatatgctgt tgctgcactt tgtatttgct
     4621 taggactctt acacagcgtt ttcgttcgtg tcctcctgta gcttctggtt gatcttctgt
     4681 attttccgcc ggtagacgct gtactggctg agccagatgc tgagatagat gcagaggaaa
     4741 attcccagat agagcagcat tccccacaga ctcagatcac accagcccag cagcagtgag
     4801 acgacccaga acaggggcgt aaccatgcag aaatgggtgg cggtggcacg gaataaactc
     4861 cacgattcca cttcccagat gacggaactg ccggcaaaaa cagaacccac cagcccggac
     4921 agcagaaatt gtgccgtggc gccggcggtt tcagagccga acagagcagt acagccgggg
     4981 ctgcacggcg catatgtgcc gatgcaaaag gaaatcacca gaattataag gctgctgatg
     5041 cacacgccta tcgggaagcc gatagcgctg cggagcagga tctgtttttt catatttttc
     5101 ataaagcgac ctccttagag tccgagtatg gatttgatgc ggttgacata gcgcctggaa
     5161 acatatgcgg tctcaccggt tttcagctgg atgccgatgg tgccggtgat gctgaggtcc
     5221 agccgtttca caaagcgcag gttgatgatc tccgatttgg agatgcggac aaaggttttt
     5281 tcgtccagca gctgttccag ttcatagagc cgctggcgca aataaaattc accatggggc
     5341 gtttgggcgt aaaccttttg cgatgcgccg taaatgcgca tgatctggga cttttccagc
     5401 aggacagcgc tgtctgcatc atagcctatg acagaatcag aacgcttttc ggacagcttt
     5461 tccaccagtt cccggacttc ggcggtcacg gcggcggtgc ggatcaaaat ttccggctct
     5521 tcgcattggc tgtccagctg tatttgtatt ttcacgtggg atgtctcctc ctttcgctgc
     5581 ggtcgactgc cagtctatca tagcatattt ttcacggcgg ctcaagccat tttcgacaac
     5641 cggtatgttt ttgcagataa acggcagaaa aaaagagatc tgtctcttga caaatctctg
     5701 tgagtacgga ataataaaag ggaagagagc ataccagtag acggtctgct ttcactgctt
     5761 ggattaaaaa gatttaaccg ccacagttgg tagctagggg cggttatttc tttttcttct
     5821 tgtggtgatg ctttttactt ttttttgatt gtagtaaacg tgaaaaatca aactcgtgga
     5881 atctgtaggg gcgaacagtg ttcgcccgtt tcttttgagg tgagtcccgg gcgaacgatg
     5941 ttcgccccta caataagaac gcattgcgga caaatctgta gaaattattg ttcagcagac
     6001 gcactggagg gatggttggc tgcggcgaaa a
//
LOCUS       DAWENO010000125         5952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5234_length_5952_cov_10.370867, whole genome shotgun sequence.
ACCESSION   DAWENO010000125 DAWENO010000000
VERSION     DAWENO010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_5952_cov_10.370867"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2302..2311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1380 a         1732 c         1581 g         1249 t
ORIGIN      
        1 tacatgggag ctggaaatca aaccgtccac cgttttcggt atgccctagg gtattattat
       61 accacatcct gccgaaagct gtcaagaaaa atcaaacctc acaaacgcaa aaaagcggtg
      121 tacttttcag tacgccgctt tttcacttct tctattttcg ggaaagcata aatcagaaac
      181 gaacgcttac gaatgtgtag agtagttcgg tgcttccttg gtgatctgaa tgtcatgcgg
      241 atggctctcc agcagtccgg cattggtgat ctgcacgaac tttgcctttt cgtggagcag
      301 ctcgatggtc ttgcagccgc agtaacccat accggagcgg ataccgccca ccagctgaaa
      361 tacagaatcg gcaacactgc ccttatacgg tacacggcct tcgacaccct ccggaaccag
      421 cttctttgcg ccttcctgga agtatctgtc cttggaaccg tttgccatag cagccaggct
      481 gcccatgcca cggtatacct tgaaggaacg tccttggtag atctcggtct ctcccggaga
      541 ctcctcgcag cctgccagca gcgaacccag cataaccaca tttccgccgg cagccagtgc
      601 ctttacgata tcgccggagt acttgatacc gccgtctgca atgaccggaa tgttgtgctt
      661 catggcaacc tgtgccacgt catagactgc tgtgatctgc ggtacgccga tgccggcaac
      721 gatacgggtg gtacagatag aacccggtcc gatacctacc tttaccgcat ctgcgccagc
      781 ctcgatcaaa gcctctgctg cctctgcggt agcgatgttt ccggcgatga ccggaacgtt
      841 cgggaaagct gccttgacct ttttcagcgt agtcataatg ttcttgctgt ggccgtgtgc
      901 agagtccagc accagaacgt ctacctgcgc atccaccaga gcgtgcgcac gctccagcac
      961 gtttgcagtg atgccgatgg cagcgccaca gagcaggcga cccttttcgt cacgggcaga
     1021 gttcgggaac tgtactgcct tttcgatatc cttaatggtg ataagaccct tgagatagcc
     1081 ctcgccgtct accagcggca gcttttcgat cttgtgctgg cgcaggattg cctgtgcctc
     1141 ttccatggtc gtgccgaccg gagcggtaat caggttttcc ttggtcataa cttcagagat
     1201 cggaccgtta tagtcggaca gaaaacgcat atcacggttg gtgatgatgc ccaccagctt
     1261 gcccacattg tccacgatcg gaacgccgga gatcttgtac ttgcccatca gcgcatttgc
     1321 atcggatacc aggtgatttt cggtcagaga gaacgggttg acgataacgc cgttttccga
     1381 gcgctttacc ttgtctacct cgtctgcctg ctgttcgatg ctcatatttt tgtggatgat
     1441 accgatgccg ccttcacggg cgatggcgat cgccattctt gcctcggtca cggtgtccat
     1501 tgccgccgtc atgatcggtg tgttcagata gacattgccg gcaagtcttg tacgcaggtc
     1561 gatcatattc ggtgttacgt cagattctgc cgggatcagc agcacgtcat cgaatgtcag
     1621 cccctgcttt ccaaactttt cacacagtcc tgtttccata gaatgacctc ctcaatcttt
     1681 ccagatcagt tctgcaaagg tctgccgcaa aaagaaacag agtggtgcgt gtccttcaca
     1741 aagctgtctt tctgttgtgt tttcggagtc cgttcacagc atcgccgcag catatgcccg
     1801 gcaacgccga tgcccggatt ccagagaacc tgtccgcagg ataggcagac aggttcgtca
     1861 tcaggttatt gtatttatca tactcttttt ttgtcggttt gtcaagggtt tcggcatcta
     1921 caaggggaat tttacagctt ttccaaaatt ctgtcttatt ctgtcagctt gaaaaaacat
     1981 accgtagggg tcgatgccca gcccgtcgcc cagcggaaat ggcgacggca gacttgccca
     2041 aggatagcaa gccacatcga cccgtcccat ttattgtagg ggcgaacatt gttcgcctaa
     2101 gactcctcag tcagcctacg gctgccagct cctctagaag aggagccaat ttagcctcct
     2161 ctcttagagg aggtgcccga agggcggagg agtttcgggc gaaccatgtc cgtcccattc
     2221 gcccctacga gataatttcc accgaactat tacaccaccg cccggcttta agaattcttt
     2281 aagaaacctg ccttatacta tnnnnnnnnn naataacagg aaaggcagaa ggtttctgtc
     2341 tgccgccgct tctcattttc caatttctgc cctgcggcaa agcgccgctg ttccgaaggc
     2401 aggggaaaac actccgcaac agctcgtaac aaaggagaag tgaacaacat gaaaaaaaac
     2461 tcgcaccgct atgtgcggaa aaccgcagcc ggcgtggtcg ctctgtccat gctctgcgcc
     2521 gctgcaatcc cttcggttct ggctatgtct gccggcgcag ccagcgcatc cggcgacctg
     2581 aacggtgacg gctctgtcac tgccgccgat gcggcaatcc tgcaaacctc actcctgggc
     2641 ggcactgccc tcaccgcaga acagtacgcc aacgccgatg tcaccggtga cggcgctgtg
     2701 gatggtctgg atctgacccg gctccgtcag atggctgcgg tcgttcccgt aagcgatgcc
     2761 atcgccatcc acctcagcga cagcggtatt accgtagagg gcgacaccaa gggcgtgacc
     2821 gccgtttccg gaaagactgt caccatctct gcctccggca actacaccgt agacggctcg
     2881 atcacagacg gacagattct ggtgaacatc ccggatacca cagcggattc tgacgcagtt
     2941 tccctgtatt tgcaggatgt gaccatgacc tcttccaacg gtgcgccctg cattctggga
     3001 cagagcgcaa agaaactgaa actgacctgc tccggtgaaa atacgctgac cgatactgcc
     3061 gccgaggcaa acgccgacac cagcggcgtg atctacggcg actgtgatat tactgttacc
     3121 aagaacagta ccggcacgct gaacatcacc agcagtatga atacggctat ccgttccaag
     3181 gacgacatca agctcaacgg cggcgctgtc gtgatcaata ccgatgtgga cgacacttcc
     3241 gacgccgatg ccatccgggc aaacaatacc ctggaggtag acggcgcatc ggtttccatt
     3301 acctcttccg cagacggtct gaaatcctcc aaggaagacg tttccatcct ctctggcaag
     3361 gtgagcatca aggcaggcaa tgacgcagta caggcggcaa ctgccctcaa tatctccggc
     3421 ggcacggtga tcgccagcgg cgaccgtggc tttaccctgg atgaaaacgg cacactcgcc
     3481 attaccggcg gcgatgtgct ggcgacagcc accgactacg cattcggcat ggattcttcc
     3541 ggtgcggcag tgactgtaga cacttccggc tgcacccagg gcgtggtaca gctggactat
     3601 gcggcagagt ggaaaaagag caacgctgtg actttgaaga agggcagcga tactgtcttt
     3661 gaaatgacgc cgaataagaa gtacacctat gtgctggcaa gcggcgcaaa gctctccggc
     3721 acagacagct ataccctcta taccggcggc acgcagatga accacagcgg ttcggacaac
     3781 ggcacatttg ccatgaccgg tacgctgacc aagttcaccg atgtcaagga actgaccggc
     3841 ggcagcgaac tcccggatga tgacagcgtt gtcactgccc tggtctacag cggcagcagc
     3901 gtgacggcaa ccaacgcctc cggcggcgta gtctccaatc cgtccaatct gaccatctcc
     3961 ggcgcaaatg tcaccattac ggcatccagt gagatttctg tcagcggcga aagcaccagc
     4021 ggacagcttg ccgtcaacgt ggacaaaacg gcagagccgg agggcaaggt ggtgctgaac
     4081 ctggagggac tgaccctttc caacagcagc gctgcgccca tctatgttga ggcgatcggc
     4141 gatgaggtgc agatctctgc caagaacggc acgaccaaca ccatttcgga cggcacatcc
     4201 cacacggata cctatgtgga cagcgacggc aataccaaca ccgtaaacgg ggcgatcttc
     4261 tcccgtgacg atctgaagct caagggcaag ggtacgctga tcgtcaacgg caataccgag
     4321 gacggcatcg tctgcaagaa cgatctgaag atctggaacg gcaatattac tgtcaacgcc
     4381 gcagatgacg gcatccgtgg caatgattcg gtacgcatcg gcgacccgga tgccgccgat
     4441 tacagcagcc tgtctgtgac cgtaaacacc aataacggca gctccggcgg cgacggcatc
     4501 aagaccaact ccgacgagga cggcaagggc tatgtcacca tcaacggcgg cacggtcaac
     4561 atcgactcct acgcagacgg catccaggca gagcagacct ttaccatgaa tggcggcgaa
     4621 ctgaacatca ccacctatca gggttcgaac tttaccggca gcgcatccgg cggcaacggc
     4681 ggctggggcg gcggcatggg aggcaatgac ggcaactcca acaagaccga catcagcgcc
     4741 aagggcatca aggctgtggg tgtgtacgat gaggcaggca cgacctggca gagcggcggc
     4801 gatctgatcg tcaacggcgg cacgatcacc attgattctt ccgacgacag tctccactgc
     4861 ggcggcgata tgcagctgct gggcggcagc atgacactgg cgaccgcaga tgacggcgca
     4921 cacagtgatc atgctctgac catcggcagc accggcggcg attatgatgc gccttatgtg
     4981 aacatcacca agtcctacga gggcatcgaa ggcgtggata tcacccagaa cagcggtacg
     5041 gttatggtaa cctccagcga cgacggctat aatgccgcag gcggcgcaga cagtagcggc
     5101 aacaataaca gcggcggctg gggtcaaggc agctggggag gacccggcgg cggaagttcc
     5161 agtgacggca gccagaccat gacctttaac ggcggctatg cctatgtgaa tgcagccggc
     5221 gacggtctgg actccaacgg caatatctac ttcaacggcg gctatgtctt tgtatcccag
     5281 accggcggcg gaaacggccc gctggactgc ggcgacagca acaactccat cacctattcc
     5341 ggcggtacag tcattgcggc aggcagcagc gatatgtttg aaacacccag cagctactcc
     5401 ttcctgtcta cgacctctgt ttctgccggt cagaccatta cctttaccga tgccagcggc
     5461 aatgtgcttg ccacattcac cctgccgaac ggctctgcgg aaatggtaat gtgcagccag
     5521 gaatccagcg tcacctgcta taccggtggc acactctccg gtacgaccta ttttgccagc
     5581 caggacagca cgaaccgctg cggctacggc ggaacgatca gcggcggtac ggccgtatct
     5641 gctagttccg gcggcaacag cgacccggga ggcggcggaa ataaccgccc gttctgagca
     5701 caggcaatct gacgctttgg cataaaacag ctatctcctt gaaatatgcc tattttcagg
     5761 taaaaaacat tcccctcatc caaacccttc tgcacagatg tgcgtgcaga aaaaacggag
     5821 acaccgagtc agatcgggtc tccgtttttt gttttcttga caggttttga tggaatgtgg
     5881 tataataaga gtccagagca taccgaaaac ggtggacggt ttgatttcca gctcccatgt
     5941 atttgatacg at
//
LOCUS       DAWENO010000126         5848 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5334_length_5848_cov_12.029518, whole genome shotgun sequence.
ACCESSION   DAWENO010000126 DAWENO010000000
VERSION     DAWENO010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5334_length_5848_cov_12.029518"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    618..627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1358 a         1523 c         1542 g         1415 t
ORIGIN      
        1 ccccctgtcc cttcgggaca tcccagcaca aggcacaccc ttcgggcgcc ttttcgaggg
       61 ggactatctt aaaaattggt ttgtttactt gaaaaaaact taccgtaggg gcaaacatgg
      121 ttcgcccgga actccaccgt caagcctacg gcttgccacc tcctctatga gaggaggctt
      181 tgagaaaaac gtctaccctt attccaatta ctgcaggagg tatctgaata tggcactgga
      241 tctattttgg gcattcctgg tcggcggtgc gctctgcgcc gtgggtcagg tcctgatcga
      301 ctacaccaag ctcaccccgg cacgcatcct taccgcatac gtggtggctg gcgtggcgct
      361 gtctgccgtg ggactctatc cctggctggt ggaactcgcc ggcggcggcg caagcgttcc
      421 gctcaccgga ttcggcaatc ttctggcaga gggtgtccgg gaatccgtgg acgaaaaggg
      481 ctttctgggc atctttatgg gcggactcac cgcatcctca gccggcatta cggcggcgat
      541 ggtgttcggg gtcgtctgtg ccgcagtctt tcgcccaaag caaaaataag cgccgtctgg
      601 cttgaaaaat gtgcaaannn nnnnnnntct ttattgtgca ttttgctgtt tttcaaaaaa
      661 atgcttgact tttatccgga aatgtagtat actatcatag tcgcaggaat tcaggatggt
      721 gtgcaaaaca ctgctcgaaa cctggtttga tgtggcggca tagctcagtt ggctagagta
      781 ctcggttcat acccgattgg tcgctggttc gaatcctgct gccgctacca tatggcccgt
      841 tggtcaagag gttaagacgc cgccctttca cggcggaatc atgggttcaa ttcccgtacg
      901 ggtcaccaaa ccacaaagct cagaaatgac gcagttgcgt tgtttctgag cttttctttt
      961 tttctctttc ctgctgctgt cctttatttg tcctttatgc aggcagattg tccggattcg
     1021 ccgaaatcga gcacagaagc aatggctgta tatgcttttg cttgtgcttg gttcagttca
     1081 tgacaataga cagacattgt ggttgtaatt gtcgaatgcc ctaatgcttc agatacagta
     1141 gcaacacctc ccacttttgt accattccca caaaacaaat caaattccaa caaaaacaag
     1201 gttgatttta tacaaaaagt gtgctataat agagactgca aagacaactt tggagaggat
     1261 gtgacgtttc cgtggaaaca acttctgaca agcactttat gaataagatc gaggttctgg
     1321 aaaatgcgga aaaggcgttt gcagaccatc agtttcagat atattatcag ccccagtata
     1381 actatctcaa ccgccgcatc gtcggcacag aggcgctggt gcggtggatc catccccagt
     1441 tcggcatcca gccgcccggc gagtttatcc cggttttcga ggacagcggt ctcatcacca
     1501 agctggatct ctacgtcatg gaagaggcgt gccggtttct ccgggagtgc cgggaatcgg
     1561 gtgtccccta cgtgccggtt tcctttaacg tgtcccggca cgatgtctat gcctgcgatt
     1621 ttgtagaacg catggagcgt atccggaaat cctatggcat cccggtgact gatctgcgtg
     1681 tggagatcac cgagagcgcc gtgatcggca gcagcgattt tctgatccgg ttcgtgtccc
     1741 ggctgcagtc tctgggctat accgtggaaa tggatgattt cggcagcggc tattcttcgc
     1801 tcaacgtgct gaaagatatc gaagtggata tcatcaagct ggatatgatc tttctgcgca
     1861 ataagatcgg cggcagaggc ggcattatca ttagttccat tgtccgcatg gcaaactggc
     1921 tgggaacgcc ggtcatcgca gagggcgtgg agaccgtgga gcaggcggaa ttcctcaaga
     1981 gcatcggctg catttatatc cagggctacc tgttttccaa gcccgtgccg gaacgggaat
     2041 atctgcgcct gctgacggag acaaagacag atactgccgt gccgggaatg cagctcagcg
     2101 ataagctgga tgcggaaaag ttctgggatc ccaccaccca ggagaccctg ctgttctata
     2161 actttgtggg cggcgctgcc attattgcct tttccgatcc ggagatcgag acccttcgtg
     2221 tcaatgaaaa gtatctgacc gagctgggca tgaatatgaa ccagcaggat gtgattctgt
     2281 accagaaaaa tctgaacttc gacaacgagc aggagcgcag agtctatatg gagaccatac
     2341 gccgtgccat tgcgttcggt gaagatgaga cctgcgagac ctggcggact gtctattcgc
     2401 cctgctgcgg agacgatcac atctgcctgt ccagcactct gcacctcatc ggaaaatcga
     2461 aggggcagta cctgttttat gtgctgattc ggaatatcac aaaggaaaag acggcatatc
     2521 tggaactggc agaaagcgaa aagaagtttc ggactgccgg agagcaggca aacatttact
     2581 gctgggaata caccattgat acgaaggaga tgcgtccctg cgcacgctgt atgcgtgacc
     2641 tgggactccc gaaactgctg gagaattatc cggagccggt gatcgcatcc gggttgtttc
     2701 cgccggacta tgccgagatg taccgggaat ggcacagaaa gctggcgcag ggtgcgatgc
     2761 acctggaggc agtgattccg ctcaccgccg accgtatccc cttccatgtc cgctataccg
     2821 ccgaactgga cgaaaacggc agaccctata aagcctacgg ctccgccacc caggtggtgg
     2881 acggcgaggc gtaaatgctt tgttttgcaa gcggcaatta cctgtccaaa cacaccgtag
     2941 gggcgaacag cgttcgcccg agattttctg actcctccgg cacgtagcgt cgaagggatt
     3001 aacccctcag tcaagcctac ggcttgccag ctcagcacaa ggcacgccct tcgggtgccc
     3061 tgttagggga gccaaaagaa cagtctcctc tcccagagga gatgcccgac agggcagagg
     3121 agttgccttg tcccccttga aaaggcgccc gaggggtgtg ccttgtgctg ggatgtcagc
     3181 gtagctgaca gggggatttg tagggggcga tgcccacatc gccccgttcc cttttttgaa
     3241 aacgaaaatc gtaggggcga acatcgttcg cccgctaact tttcccattt caggcaaccc
     3301 tgccctaggg aaaactcgtg caacccgtcc ggcggaatcg ctcgtacctc gctgtccgcc
     3361 ttaggcttgc aaacgccctt gtttgccctt tcctgtatgt ataacgctgg cattctgaat
     3421 atctgttttg agtatagtta ttgtttttta tacggcgttt gctgtgcttg cttttctttt
     3481 gttttcttgt gcaaaatttc tcacaaagaa tttctcttct ttttttcttt gctaaaaaga
     3541 aaaaaagaag caaaaaaaga aaagctctcg cctgcccttg tcctgtgcgg caatgtatca
     3601 cacgactggc agtgtattgc agttaaatta tgcacaacaa aaatcccccg gaggggttat
     3661 aggcggcggc tgccgccgta acactgccct tcttgaacgc taaatttggg ggaaaggtcc
     3721 gcaacgaccc gttgccgtcg ccacagattc tgcaaacttt cggtaaaatg ttgacaatgc
     3781 cacgaaaatc gggtacaata aaaaggaacg ccatttcaga atattttctg ggatgcaatg
     3841 atttttaccg cagaaaaagg agatctttgt atgaatattg ctgttgctca gtccggcggt
     3901 ccgacctgtg ccataaacgc ttctctggtg ggcgtgttcc gggaatccct caaagaaccg
     3961 gcaatcgatg ccattttcgg ctctgtgaac ggtgtcgagg gcattatcca ggatcacctc
     4021 attgatctga aaaccatgat ccgtaccaac gaagatatgg aactgctgcg ccagaccccg
     4081 tctaccgtcc tcggctcctg ccgctataag ctgccggatt accacgagga cagcagcgtc
     4141 tatgaaaaga tcgccgcagt gctgaaacgc cgcaagatcg gggcattttt ctacatcggc
     4201 ggcaatgact ccatggatac tgtccagaaa ctgtctgcct atttcaagga gatcagcctg
     4261 gacgtaaagg tcatcggtat ccccaagacc attgataacg atttgtgcat caccgaccat
     4321 acgccgggct ttggctctgc ggcaaaatat gtggcaacca ccatgcagga gatcatccgt
     4381 gacagctccg tttacagcat cccctcagtg actatcgtgg agatcatggg acgccatgcc
     4441 ggatggctca ccgcatccag cgccgtgctg cacgcctacg gcgaacctgc gccgcatctg
     4501 gtctatgtgc cggagaccca cttctctgcg gagcgctttc tccatgacgt ggagcaggaa
     4561 atggcaaagc ataaggcagt catcgttgcc gtttccgagg gcgtttctgt gccggagggc
     4621 gtgcagtccg ggctggtgga caacttcggg cataagtacc tgtctggcat cggcaagtac
     4681 ctggaacagc tggtgcgtga taccttcgga tgtaaggtgc gttctgtgga actgaacgtc
     4741 atgcagcgct gctcgtccca tttgagttcc aaaacggata tcgacgaggc agagcagatc
     4801 ggtgcagccg gcgtgcagtg tgcgctccgt ggcgagaccg gacgtgtcat gatcttccgc
     4861 cgcatccaca ataagcccta taccgtcgtg atggagccgg cagatgcctc ccagatcgcc
     4921 aaccgggaaa agttcctgcc cagagagttc ctcaccccgg caggcaataa catcagcgac
     4981 gaggcaatgt cctatttcct gccgctgatc cagggcgaac tgaatctgat gatgcgtggc
     5041 ggcatcccca tgcacttcac catccaggaa tccgtgctga agtaattcgc agtgtctgga
     5101 caacagataa aaataaaacg gacagtgttt cacgtgaaac attgtccgtt ttttgcttgt
     5161 tggagttgga aagggcgctg ggtcaacaca ccgtaggggt gaacatcgtt cgcccgagac
     5221 ttctccgtca agcctgcggc ttgccacctc ctctaaagag gaggctttac cattgtctcc
     5281 tctcatagag gagatgcccg gcagggcaga ggagttttag tctcccttga aaagggaggt
     5341 ggcacgcagt gccggaggga tatccccctg tcccttcggg acatcccagc acaaggcaca
     5401 ccctacgggc gccttttcaa gggggactat cttaaaaatt ggtttgctta aaaaagctta
     5461 ctgtaggggc gaacatcgtt cgcccatgaa ctttcccatt tccaaaaaca ctgccctagg
     5521 ttaaactcct gcaacccgtc cggcgaaatc gctcgtacct cgctgtccgc ctgggcttgc
     5581 aaacgccctt gtttgccttt tcctgtacgt gcggcgtagg ctttctgact gttgcttgtg
     5641 caggtggttt atgtaatcaa tcggcgtttg caattctaat agtctcctct catagaggag
     5701 atgcccgaca gggcagagga gtcatttgtc tcccttgaaa agggagatgt caccgtaggt
     5761 gacagaggga tttttaagtt tcttcaaaga gttactcttc tttttttctt tgctaaaaag
     5821 aaaaaaagaa gcaaaaaaag aaaagctc
//
LOCUS       DAWENO010000127         5770 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5404_length_5770_cov_8.446369, whole genome shotgun sequence.
ACCESSION   DAWENO010000127 DAWENO010000000
VERSION     DAWENO010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_5770_cov_8.446369"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    332..341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1692 a         1257 c         1428 g         1383 t
ORIGIN      
        1 gcttttatcc agatgtgcat ggatatcccg ccgaagaaga aagccgtctc cctggttaca
       61 ggaaggatcc acactttccg gcgcaacacc gccctccgca atcaaaacat ttacagaggc
      121 tccctgcggc tcatagtcct gtctggaaat attcagcacc gttgcgccga tcatctctgt
      181 gacatcacac agaatattgg tcagacgctc cgaattgtac tgttcgtcga tgtatttgat
      241 ataatcctgt ttttcacgtt ccgattttgc atagcataca tcaaaaatat taaagctcaa
      301 cggattagtg agattgttaa atccatataa gnnnnnnnnn ngttcacgtt cttacaatca
      361 aaaattattg taccacaatt tgcaggaaat tgcaatggga tttacaaaac ttttttcgaa
      421 ttccacgcag aaaaacaaca tttcacaagg tgcgatacaa gacatgaaac tgtaccgact
      481 ggcaattgtt gtttatgttt ttccattttt tgaattttca agacttacca cttgcaaaat
      541 acagcgaagt atagtataat agaacaaatc gaatggaact gcggcgaatt gtctgcaagc
      601 cttctggcag aaagacacag ctgatatgtt tgggagagca taggagttac tgcgatacgt
      661 ttcacgattt gccggaacag cattaaaaca ctgtccggca cgaaatgctg atgcagaaag
      721 gaagaacaag aatcatgaac gatacgatga atgatgcaaa agatacaaca gaaatcgctg
      781 aagaaatgga tacaccaacc gtactgacac tgccgctgct tgctatgcgt ggcattgtgc
      841 tgtttcccgg catgattatg cactttgacc ttgcaagaga accttttgtc aaggcactga
      901 aagccggcat ggaatcggat cgccgtgtag ttctggtaac gcaaaaagat ccattgatag
      961 aagagccgaa accggatgac ctttatactg ttggcgtcat cgctgaagtt cgccagattc
     1021 ttcgtgcgcc ggacggtgtt acacgtgttc tggtagaggg aaaagaacgg gttgcaatta
     1081 cttcggcagt tcttgagtca gataaaaaag acaagtcatt ttatccccat gccgaattca
     1141 aatatgctcc ggaaacgccg ctggacattt ccgaccccca cgaagtagct gctgtttcca
     1201 gatgtattaa ggacattttt caggaatatg cagacttgct ccctcgaatg ccgaaagaac
     1261 tggcagcaac cgtattctgt gagcaagatg acacgaagat gtttaacgag atcgtgttca
     1321 acctggcttt tgactatgtg gaaaagcagc gcctgctgga agaaaacgat tggctggaac
     1381 ggctgaaaat gctgtatcag acgctggtag acgaaatgga tattctccgg atggagcgaa
     1441 agatccagga gaaaacacag gaaaacctgg atcgctctca gcgggaatat tatctccggg
     1501 aacagatgca ccaaattgcc gaacagctag gagaatctga agatccggcg gcggaaatgg
     1561 aaacatacac ggatgccatt gccgcactgc ccctggatga tgccagccgg aaaaagctgg
     1621 agaaagaagc ggagcgtctg tcacgactgc cggctgcatc tcaggaggct tttgtgattc
     1681 gcagctatct ggacactgtc ctggctcttc cctggaacaa atccagtcac acaaagccga
     1741 atctgaaacg ggcacaggcg gttctggaac gggatcacta tggactgcaa aaggtcaaag
     1801 aacgaattct ggaaagcatt gcagttcgta cactgctccc ggaagcaacc ggacaaatcc
     1861 tatgttttgt aggacctccc ggtgtcggaa aaacttctat cggacgttct attgcaaagg
     1921 cactgggtcg tcactatgag cgtgtttctc tgggcggtgt ccgggatgaa tccgatatcc
     1981 gaggacaccg caagacatat gtaggcgcta tgccagggcg gatcatggat gcgatgacaa
     2041 gagccggcac aaacaatccc atgattcttc tggatgaagt ggacaagatg agcaacgatt
     2101 tccgaggcga tccgtctgcc gcattgctgg aagttctgga cagtgaacag aacaaagcat
     2161 ttcgtgacca ctacattgag gtgccgtttg atctcagtaa ggtgtttttt gttgcaactg
     2221 caaactcgct ggatccgatt cctgcgcctc tgctggatcg tatggaaatc atcgaactgg
     2281 gcagttatac ccgtgaagaa aaatttcata ttgccaaaga acatcttgtc aaaaagcaac
     2341 tgaaaaagca tggactgaaa ggaacgcaat gccgtattcc agacgaagtt ctgtatgcga
     2401 ttattgacgg atacacaaga gaagccggtg tccgtgaatt ggagcgaact atcggtacgc
     2461 tgtgccgcaa ggcggcgaaa aaaattgttg acggtgactg caaaaaagtt actttcacag
     2521 cagagacact gaaagactat ctgggcattc aaaagttccg tccggacgaa caaattcacg
     2581 aggacacggt cggtgttgtc aacggacttg catggacatc cgtcggtggc gtcttgatgc
     2641 ccatggaagt actggtaatg ccgggaaagg gcgcagtgga atacaccggg tctcttggcg
     2701 atgtgatgcg ggaaagtgca aaaattgctg tcagctatac cagaagtgtg acaaagaaat
     2761 atgatattcc ggaagatttc tataccaaat gcgatctgca catccatgca ccggaaggtg
     2821 cggttccgaa agatggtccg tctgccggtg tcacaatgac cactgcgctg atctcagcgc
     2881 tttccggccg tcctgtgcgg ggagatgttg caatgacagg cgaaatcacg ctgcatggaa
     2941 aagtactgcc gatcggcgga ttgcgtgaaa agtcaatggc agcttataaa gccggcattc
     3001 gaacagtaat cattccggaa gataacatac ccgatctgga agaaatagat gaaacggtac
     3061 gcagcagcgt ccatttcatt ccggcaaaaa cgctggatac agtgctgcaa aatgcgttag
     3121 tccccacagc gaaaaatgta cccgagacag cggcaacaga aatgtgtcat gtgtaaacat
     3181 agaaggagaa agtatgcagt ttcagaatgc tgaatttata gcgtcctatg gaacattttc
     3241 acagattcct ccatgtgatt ctgtagaaat tgcttttgca ggccgctcca acgtgggaaa
     3301 gtccacgctg attaacaaga tttttcaacg aaagtcgctg gcaagagtca gcgcagtgcc
     3361 gggcaagacc gcaacgatca atttctaccg gctgcgcccg ctgaccatcg tggaccttcc
     3421 gggatacggt tatgcaaagg ttgcaaagtc tgaaaaagca aaatggtctg gtttgatttc
     3481 cggatatctc ggcgcagatc gtgatttaag gctcattctg ctgctgattg atatgcgtca
     3541 tgcaccctcg aaggatgaca ttcagatgat tgatttcctc gtggaatcag aacttccttt
     3601 tttgatcgtg ctgacaaaag ccgacaagct gaataagaca gaacgtgcca aacggatgca
     3661 ggcatttgag acagaaattc cgtacttttc ccagattcac gtgattccgt tctctgcagt
     3721 caccagagaa ggcgtcagcg aagtccaggc ggtgctagag gaaattgcat ccgaaacaga
     3781 agagacagaa tgactttatt tctgtgcttg aactgaaaac gattttcaag caggcagcag
     3841 aaactaaaat cataatgcaa tgcagaaagg aagtataaaa atgtttgtat ctgacaatct
     3901 tgatgtcaac aaagcaggaa atctgacaat tggcggtgta gacactgttg cactggcaaa
     3961 gaagtacggc acaccgctgt atgtcatgga tgaagatatg attcgccgtg actgcaagat
     4021 tttccagaaa agtattgaca catactacga cggacaggga atggtctgct atgccagcaa
     4081 agctttttcc tgcaaagaaa tctatcgtgt catgcagtcg gaaaatatgg gaatcgacgt
     4141 tgtttccgga ggtgaactgt acaccgctgt acaggcagga tttgatgtga aaaaagcttg
     4201 ttttcacgga aacaacaaaa ccacagaaga actgaacatg gcgctggatt atggcgttgg
     4261 aagaatcgtt gtggacaata tctacgagat ggaacagctg aaccatctgg cagaacagaa
     4321 aaatctgaca gcgcccatcc tgctccgtat caagccgggt gtagatgcgc atacacacaa
     4381 ctttattcgc accggacaga tcgacagcaa gtttggtttt gcattagaga ccggagaggc
     4441 ttatgatgct ataaaaaaag caatttctta tccgcatctg aaactgcgtg gtctgcactg
     4501 ccatattgga tcgcagatcc tggacacaca gggattcacc gcagctgcag aagttatgat
     4561 ggaattcatt gaaaagctga aaaaagaatt gggttatgag gcagaagatc tgaaccttgg
     4621 cggcggcttc ggtatccgct atacaaaggc agatgatccg ctgccgtatg atgcttacat
     4681 ggaggcagtt gcaaaggtaa tcaacggatt ctgtgcgtcc catgatgtaa agagaccgtt
     4741 tatcttgatt gaaccgggtc gttccattgc agcaccggca ggtattacgc tgtacaccgt
     4801 aggcggcatc aaggaaatcc cgaatatccg tacctatgtt tccattgacg gtggtatgtg
     4861 cgacaacccg cgttatattc tttatcaatc tgaatatgat gcactggttg caaacaaagc
     4921 agagcagcct aagacggaaa aggtcaccat cgccggcaag tgctgtgaaa gcggcgacct
     4981 tatcggtgaa aatatggaga tccagaagtg cgaagtcggt gatattatcg ctgtatgcgc
     5041 tacgggtgct tataattact ctatggcgtc caactacaat cgtctgcaaa agcctgctgt
     5101 tgtctttgtc aaaggcggag aagacagact ggcagtcaag agagaatctc tggatgatct
     5161 gctgagaaac gatatctgag tattctgatt tacacagtca tttttttaca acaaccgttc
     5221 ttgtcttggc aaggacggtt gttgttgtta tagaaaaatc agctttttca tttacagctt
     5281 gcaatccaaa aaaatatatg ctataatagg tatattgcaa ggcggttttc cgctgaaata
     5341 caaggaggaa caaaaatgcc acatattacg atcaaaatgc tgaaaggcag aacagacgaa
     5401 cagaaaaaac aggcagcaga caaagttgca gctgcactgg tagatgcaat cggctgcaat
     5461 ccggaacata tttccgttgc tgtcgaggat tttactccgg tagaatggca ggatgaattc
     5521 cgcaaggaag tcaccgaaag cagccatatc tataaggaac cgcactacga tccgaaggac
     5581 gtactgtaat ggggttgatt ttagcttccg catcgccacg gcggcaggaa ctcctacggc
     5641 tgattacgaa tgactttaca gtttttccgg cagctgtaga tgagtctctg ccgcctgcgt
     5701 tgtctgtcga taatgccgca caatatctcg ccagaaaaaa agcagcagcg gtttcttctg
     5761 cttttccgga
//
LOCUS       DAWENO010000128         5730 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5450_length_5730_cov_9.708194, whole genome shotgun sequence.
ACCESSION   DAWENO010000128 DAWENO010000000
VERSION     DAWENO010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_5730_cov_9.708194"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1148 a         1773 c         1473 g         1336 t
ORIGIN      
        1 gccgtcacgt tttgcagtcc caggcgcttg acgttgtcct ggatcagctt gacccgatgc
       61 gccgccagat caaaggcgta caccatgccg gactgcgcca tgcctgccgc aatggtacag
      121 gtctttccgc ccggtgcggc gcagagatcc aggatcgttt ccccgggctt cgcccccaac
      181 gccagagcgc agatctggga agagagatcc tgcacataga accagccctt acggaattcc
      241 ggcagctttc ggatatcgcc ggtgtgcagc tcatatgcgc cgggcagatt tgtgggactg
      301 acggcatctc ccaatgcctg ttccagttct gcaccggtac acgccgccgg attctgccgg
      361 ataaaccggt ttgccggctg caaagcgtcc gccagaaatg cacgggtcgt ctcttctcca
      421 taggcatcca gcagcttttc cagcagccac tgcggtaccg catattccac tgccattgcc
      481 gcctttttcc ccttgggata cggcactttg cactcgtccc ggagaaagcc gcgcagcaca
      541 gcgttcacaa agccggatgc gctggctttc cggcaggttt tcgtcagctt tacgctctca
      601 ttgactgccg cacgggacgg gatctggtcg cagtacagca gctgataaat gcccagacga
      661 agaatgttcc gcacggtaca gtccagcttt cgcagcggct gcttgctgta gtgggaaatc
      721 accgcatcca gggtaatacg ccgttcgatc acgccgtaat acagagatgc gcagagccgc
      781 ttatcccggt cgtccagctg tgactgggac agtgcgctgt ccagcagcag gttggaatag
      841 ctctgctcct gttcggttcg gttcagcagc tgtactgcca gttttcttgc actcatcttc
      901 ggttgttcct ctttgaaatg ttcagcagac gaatcagctg caaaatgctg ttgagcaggg
      961 ctgccacata agtgagcgct gccgctgtca gcactttccg tgccttggtc agttcctcac
     1021 cagacaaaat ataccggctt tccagcgtct gcatggcacg gctgctggcg ttgaattcca
     1081 ccggcagggt cgccagctgg aagaatacga taagggaaaa cagcaggatg ccagccatct
     1141 gcagaggcat tccggcgtag gaattgctga agatcatgcc cagcagaaag acgatatacc
     1201 acagccggga accgatctgt gtcaccggga taatggcgct gcgcacctgc atgggaaaat
     1261 agtccacgga atgctgcacc gcatggccgc actcatgggc agcgacaccg atcgccgcca
     1321 ccgaggtgga atcatgcacg ctgtctgaca gccggatcac attgcccttc gggtcgtagt
     1381 gatcggtcag atttccgctg acccgttcga tccggatctg atataaaccg ttgtcgtcca
     1441 gaatctgacg tgccacctgc gccgccgtca gtccacggct gttgtggtcg ttctgatacc
     1501 ggcggaatgt ggatttcaca ttccaggatg cccacaagct gacaaccagt ccgatcagta
     1561 ccagaccata tccaacgtca aaccatccgc cataaaacag cataagattc cctcactctc
     1621 tctggtttat cccagttttt cacctttttc cagtttcttt ccacgcagaa aatctgccgt
     1681 ctgcatccgg cgcttgcctt ccggctggat ctccgtaaaa cgcagtccgt ttccgtcgcc
     1741 gcaggcaacc acaaacgccg ccggatctgc aatggttccg gcatcgccgg agacgccttc
     1801 gaccacacgg gagccgtaga ccttcagacg cttgccctcc atctgggtga accctgtgat
     1861 gccacgaatc agattatgca gttctgccgc cggacgggta aagtccagcg cagacatttc
     1921 cttggtgatg cgtgacgcat aggaggactg tgcatcgtcc tggggtgtgc cgtggagtgt
     1981 gccagcctcc agctgtgcca gcgtctcctc cagcgtttcc acgcagagtt ccgacagacg
     2041 gctgtacagc tcttctgcgg tctcttccgg accgatcgct gtttcctttt tcaccagcat
     2101 atcaccggtg tccagtccct ctcccatctg ctgtgccgtc acgccggtct ttgtttcgcc
     2161 gttcagaatc acccactgaa tgggtgctgc gccacggtat gccgggagca gggatgcgtg
     2221 aagattgata cacccatact ttggcagttc cagcacctct ttcggcagaa tctgtccgta
     2281 tgccagaacg acaatgagat ccggattcag ctggcgcagg gtctccagcg ccgctgcggc
     2341 atcttcgccc tttcgcagcg acaggggctg atataccgga atgtcatatt ccagcgccgc
     2401 cgccttgact gcggtgggga tggtatggtg accacgtccc ttgggacggt cgggctgggt
     2461 cagaacgccc acaacctggt gcttgctctc tgccagatac cgcagtgtcg gcacggcaat
     2521 atccggcgtt cccatataaa cgattctcat gcttcttcca aatcctccgg gtctacaaat
     2581 tcctctacca catcaatgaa cagctgaccg tccagatgtg cgttttcgtg acaggtacac
     2641 tgtgcgccca gtccgctgag tgtcatctcg taccactcac ccttccggtt ctgcgcccgg
     2701 aacttgcact tgttcgggcg tgtcacatag ccccatttat tcggcacaga aaggcagccc
     2761 tccagaacgt gctgctttcc gctggttttc agaatttccg ggttgattgc ctcgatcttc
     2821 atctcgccca ggtgcataat gaaaaagcgg cggcaatagc ctacctgggg cgcagccaga
     2881 ccgacaccgt tcgccttgtc cagagtctcg tgcatatcgt ccagcagctg gttcagcttg
     2941 tccccgaact ccgtcaccgg gcggcatttc atgcgcagga tgggatctcc ttcttttata
     3001 atacgtcgaa ttgccataat gattcctcca tattgtcttc accggcgctc agacgccgac
     3061 atcaccattc atatccgcat acagatgcac tctcgcaaag caccggtctg cccctgcctc
     3121 tgtcagcacc gcccggatca gcgagcggta cggctgggtg tttttgcact tgatgaccag
     3181 ctgatagcgg aactttccgt tgacccgccc ataaatgcag ggaaccggtt ccagcaccca
     3241 gatgggatag gaaaagccgg ttttccggat ctgcgctttc agcacatctg ccgtgtgcat
     3301 cgccgccgtc cgcacttcct gttcccgcat tccggtaaag ccgatgacgc agatatcgca
     3361 gatcggcgga aagatcagct gacggcgcag tgcgatttcc tgggcataaa actccggata
     3421 cgcctgctgt gccgcaagcc gcagaacata gtgatcgggc atactggttt gcaggatggc
     3481 acgtccggtc ttatcgcctc tgccgcctct gcccaccacc tgggtgacca gggaaaaggt
     3541 tcgctcatag ctgcggaaat ccccggcata cagcgccttg tccacggaca ggacgcctac
     3601 cagcgtcacg tttggaaaat ccagtccctt tccgatcatc tgcgttccca gcatgatgtc
     3661 atactttccg ctgcggaact cgttgaaccg ttcctcatag gcataccggg acatggtggt
     3721 gtcggcatcc atccggagaa cacgggcatc cggcacaatg gctttcagtt cctcctccag
     3781 ccgctgtgtg ccgaatccca ttttccgcag atgctcaccg ccgcaggacg gacagtgcgt
     3841 caccatttcc tgggtatgtc cgcagtaatg gcacatcagg ctgtcattga ccttgtggta
     3901 tgtcatagga atgctgcaat tgggacagta caccggctgg ttgcaggaag cacagctaat
     3961 gatcgtatga tagccccggc ggttcagcag cagcagcgac tggttcccgg cgtccagatt
     4021 ctcccggagc gcctgtgcca gtgtttccga aaacatcccg tcatttccgg cagttctctc
     4081 ttcctgcata tccacgatct ccacctgcgg cagcggcgcc tggttatacc gctgtttcat
     4141 ttccaatagg gtatagacac cacgttttgc atagtaatag ctttccacag acggtgtcgc
     4201 cgatgccagc agcagcggac actggtgcgt ctgacagcgt ttttttgcca cagaaatggc
     4261 gtgataccgg ggcgcactgt cggatttata cgtccgctcg ccctcttcat ccaccacgat
     4321 cagtcccaga tcctgcactg gtgcaaagac agcacttctc gtgccgatga tgattttcgc
     4381 ctcgccggat gcgatgctgc gccaggtctc actgcgctgt cccagagaca attcgctgtg
     4441 gatcaccgca acaatatccc cgaacaggct gtaaaaccgt tccatgatct gcggtgtcag
     4501 cgcaatttcc ggcaccatca ggatcacctg tttgccacgc tgtaccactg tgtcgatcag
     4561 tcggataaac acgctggtct tgccgctgcc ggtcacgccg tgcagcagaa agcagtgcat
     4621 tccctcttcc agggctgcga ccatccggtc atagactgcc tgctgttccg cagacaggat
     4681 gatatccgtg ggctgttgag tttgtccgcc acttgcatac gagtgcatcg gcggtttcac
     4741 cgtatagctt tccactacgc cgttctgcac cagctttttt gccacagccg atgtcacgcc
     4801 gctgagatag cacgcctcct tttccgaaag cgcatctacc tcctgcaaaa tgcgtaccag
     4861 cttctgctgt gtcttggtca ccggcggcag cgcatccggc ggcactgcca tccggagcat
     4921 cttgatgccg gtctgtccgt gggtcctttc ctttaccgtt tccacccggc gcagaaagcc
     4981 tttctgcacc agagattcca cgattttctt gccctggaca tcggcgtccg ccaaaatgcg
     5041 gcgaaactcc tgcttgttct tagcacggcg cagaaagaac agcacgtgtt cctctgcctc
     5101 agtcagagcc gtctccggct gcggcgagac aagtgtgatc gtctccacca gctgcacctg
     5161 cattccagca ggcaggatgg tgcgcactgc atcgtaataa gtgcagaaag tcatttcctt
     5221 gagccagaat accatgtcca gcatctccgg agagaggatc ggctgtttgt ccactacgga
     5281 cagcactggt ttcagctgct gttccgtctg cacttcctgt gcagcagaca aaacgatccc
     5341 cacacgcttg gcattgccct ttccgaatgg tacaacgacc cggcatccca ccgggatctc
     5401 ccgggtggac acataggaaa acagacggtc aaacgtataa gacgcagcac agacggcaac
     5461 gcctacgcac cagatgtgcg gcagttcctg tgtttccatt ctgccttagt gcggcagttc
     5521 ttcgtcgtcc acgatgcggt actttccggc agccagttcc cggatcgcag tctttaccgg
     5581 cttttcttcc agggtcttgc cttcctcgta gagttcatcg gcaatctcac gggcacgctt
     5641 tgccacgccg atcaccagag aataactgct ttcgttcttt gtaatcaact gatttacaga
     5701 cggtcttaac atattcattt tctcagcacc
//
LOCUS       DAWENO010000129         5725 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5454_length_5725_cov_9.051852, whole genome shotgun sequence.
ACCESSION   DAWENO010000129 DAWENO010000000
VERSION     DAWENO010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5454_length_5725_cov_9.051852"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5490..5505
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1400 a         1414 c         1483 g         1412 t
ORIGIN      
        1 ttacagcggt gacctgctct ttgtctttgc caacggcaag gctgcccgtg ttccgctgtc
       61 tgcatatgcg accaagacga accggaaaaa gctgcaaaac gcctacagcg gtaagtcacc
      121 cctggtacag attttgcaga ttcccggcgg tgaagcctcc tgcccggtat ttatccggac
      181 aaacggcaac cgtgcagtcc tggcagagac atccctgatt gcagcaaagg cgaccaggga
      241 cacacagggc gtacaggttg tgaccttgaa agccaagcat ctggtctgcg aggcgagaat
      301 tctgaacgag gaagaatcca cggcactgaa aaagtatcgt gtgaaaacga ttcctgcaac
      361 cggcggcatg gcgaaagacc tgccgggcag cggtcagatg acattgttat aaaaagaaaa
      421 gccactcctt tttatcggga gcggctcttt tttatgacag aggatttgtc tgttttattt
      481 ttccgggaga ctgtcgatga tatgtgtcag gaaacggagc agggaagttg catcgctgct
      541 gtccagacca tcatctccgt tgcagtccgc atttttggat gcctgtgcat tcagcttgac
      601 ctgtccggca actgccttgt tcagctgaac ggcatcggta atgtctactc tgccatccag
      661 gttgacatcg ccgtaaagac ttacggaaac atcagcatcg cctgttgtcg cagaagtggt
      721 gcctgtatct tccgatgcgg aagaggtctc tgtggaaacc gttgttgttt cagtagttgt
      781 ttccgcagaa gaaacggtcg tttcggattc tgtactgctt tcgctgaccg tcgtttcact
      841 ggaagaggtt tctgtctccg gtgcagaggt tgtggtcagc gtttcagttg tatctgtggt
      901 ttcggcaggg gttgtggtgg tcgtatccgg tttgtcgttg tcatattcat ataccagagt
      961 gaccgagttg tatgttgtag tgatgtcagt accgctggca gcatcctttt ccgaggatgc
     1021 ccaccagctt tgcagctctg cggaggtgtc attgcagaag cctgcgatcg tcgtggactt
     1081 ttcgtctaca acgatggtgt attgatcgtc aaagggaatg gagacgttgt tggtgttcag
     1141 acgatactgg aacgacttgt tgccccatac ctcatccgtg ctgcctgcct ctacaacctt
     1201 gttaaagcaa agcgcaccgc cgcctacaca ccagccggag ccggcgttgg aggttgctgt
     1261 gacattgaga atgacctctt tcagatgctc cttgtcaccc agcgggatca tataattgcc
     1321 attttgattt ttctccaggt cgctaaagtt gtaggtcgtt ttcttcactt caatattcgg
     1381 ttcgtccgga atcacaacat ctgtggtctc atcggcaatt accacctgtg ccacggacaa
     1441 aggcggcagt tccacggtca cctggttgcc tgagatatcg ttctgcacat ccaggatctt
     1501 gatatcactg ctgtcctgcg taactgcgta taccactgcg ctcttataat cggtatctgt
     1561 gccgtccagg gagatgactg ccttttcggt gttttccttg tccttgttgg agacaacggc
     1621 ggtaactttg ccgtcgtcgc tgccgtcaat ggatgcaaaa atggcagcct tggacaaatc
     1681 cggcgaagag gactctacca gagtgtcccc gaaatcgctg cccttgccgt cgtaattggt
     1741 atagaggttg atggcgcttg tcacatacgg acacttggaa agcgttcccc aataggtcgc
     1801 cagatatacg ccctggtctg caaatgcacc cagcgcctcg gtctccgcaa tcgcagaaac
     1861 caccgacttg cctgtgttgt cctcgtccgc gatatttgcc agattgtatt ccgtcagcgc
     1921 cagttttgtg ccgggatagt acttgtcaat ggattcctgc atccgggtca gaaacgggaa
     1981 cgagctgccg aaccactggg aaagccagct gttttcctta tagtccgggt cgtacagact
     2041 tctcgccgcc tgtaaaatgc cgtcatccgt gctgcagtcc tgcgcatagt aatgtacatc
     2101 tacaacatcc aaaaggcgca tgccgttttc tttttcagcg tcagccatct gcttgagata
     2161 atagtccatg taccaggaat actgactctt taccgcctcc cagtccgggt cggagtagtt
     2221 gtcgccgttg ccagcctggt aacacgggag aatgccccag aaagccggac cgtagatctc
     2281 tgcgttcggg tcaagacttt tgaccacttt agaaagcgca atggatttcg ataccagctc
     2341 ctggttggaa acctcgtctt tgtgcagcag cggatgtgtg tcattccaga gtaccggttc
     2401 gttgtccaga ttgtatccct ggataccggt agaagtggtg gagtctccca ggttttgaat
     2461 cagatagttg acatactcat ccatgtaaac ggtatcgtcg gtcagatccg gtgtgtctga
     2521 tagcgcaccg tctttctgaa atgcgacctt gttccagcgg ctggaaggtg cagcctctga
     2581 atctgtgacc gttccgtttt tgtcagcggc aacatatcca gccatctgga gcgttgccat
     2641 tttgtatccg atgccctgct ccgtgcattc cttggaaagc tggtgtgcct ggtatgccgg
     2701 accgtcagag acatcaccca tgtgtgtgtc agaactgttg acccagtctt caccggcgtt
     2761 ggagtagttg gtttcccagt tgtatccggt aaagcggttt ccgccctggc ggacggcgtt
     2821 tgtcttgacg cttttcaggc tgcctgagtt gccggattcg tttacgccgt agatatacgg
     2881 gctgatcgcc tttttctcgc cgtccagctg gatcttcagc tgtatgtcat agctgccggc
     2941 ggcagagaca gataaaatgg atgggatctg tgcggcgcag gctgctgcaa gaacacttgc
     3001 ggtcagacct gcaagaatac gtttcttcat gttcaattct ccttaaaaca ataaaacaat
     3061 ggtggtttca aaaacttcga tattattata gcacacatct tttcaaaaat caagtgcttt
     3121 acacagaata tttcttcata tgcgcataat ttatatgcaa tttgcagaac aacatcgtaa
     3181 cagtattctg ccaaatcagc aggaaattgt ccttgacaaa agaaaattgc cgtgctaaaa
     3241 tgaaacatgg agaattctaa caggaggttt tgtatgagaa tatcaaagca taagcgactg
     3301 ttcggagggt tatttgcagt attgactgcg gcatctcttg tgggttgtca cgccgattct
     3361 gccgactcag gcagcaaagc agagaaaccg gcagaaaaac agccgacact gttcgatacc
     3421 tatgtttcca atactgtgat ttcaacaggc tgtaatacaa taatcaaaaa cgcagagtct
     3481 gtgacttatc gggcatggtt cccggtcgag gcggcaggat cgtttgacta tcgcttttat
     3541 ttttccaata ccgtagattc cacctggggt gacggcagcg acagctacgt gggaatgccc
     3601 ggcggaagtt acaccattga aagtgcaaag gtctatgacg gcggaacaga atttgatgcg
     3661 aatgtgacac cgtccgtttc tgtcccggtg actttttcta atggaagtac cacaaaagaa
     3721 gtccagccgg acgaaacatt ctggagtgat accgtaaata tcacaattcc ggaagatcac
     3781 taccttttat gggagtggac gatcagcggc tccaatatcc cggcaatcgc tatgtccaat
     3841 ctggcatact cctatgcaga tcatggggat ggaaaaggtt ttctctatac caatgagatt
     3901 ccggtgccgc agctggtggg ctgtgaccgg gaagtctccg caagaatcgt gaccctgggt
     3961 gactcagtta cacagggctg ccagacaaca gaatttgcac agcagttctg ggcggcacag
     4021 ctgttggatc agctgggagc ggaggattac agcctgtgga atctgggcat cggctatgcc
     4081 cgtgcctcgg actgtgccaa ggacgaagac tggctgaacc gtgcgattgc aggcgcagat
     4141 gtgattacag ttgcgtttgg aacaaacgat attatcagcg gtccttacgg cggaaatggt
     4201 cacgccacgg cggatgagat ggagaacgat gtccggaaga tcacgcaaaa gtgtaccgat
     4261 tccggcataa aaacgattct gtggaattcg ccgccgtttg atctgactcc ggaactggaa
     4321 gagatccgga cagcctataa tcagaaggtg gaaggcattg cagacgattg cggcgcatcc
     4381 tattttgacg ttgctgcact tctggcagac ccgtcagatg cggcaaaaac ggtctatgga
     4441 cagcatccga atgatgaagg cggaacggta attgcggacg cattggtgaa agttgtccgt
     4501 gaaatagaaa aaacatcaga gtgaaaggtt gattcagaat gaaacgaaga ctcacagcaa
     4561 tcattgccgg gctttgcatc gcagcacttc cggtggggaa gtgcgccgca aatgtgcttc
     4621 cgtctgacac agcggacatc gtgtcagcgg cagaaccggt gcgcatcctg gcgatgggcg
     4681 attccatcac acatggctat atcaacggag acaacggcta ccgcaaatat ctctgttacc
     4741 agctccagca gcagggcttt acggactttg atatggtcgg accgaacaac aactggacag
     4801 attctgtctc ctacaccacc gcagacggcg taacgtttga atatgacccg gcacacgccg
     4861 gatacagcgg atatgcaatc caggcgtatt ccggcagaca gggactgtac gaaacggtgt
     4921 ttgacacgac ctataccaac ggagatgtct ccggaaatat gatggaggca tatgatccgg
     4981 atatcgtttt gctgcaaatc ggcaccaacg atcttttgga caatcacaat gacggcatca
     5041 cagaccgcct ggaaaccatg gtggacaaac tgctggacag catggacgac caggatatgc
     5101 ttttcgtggc atctgtgccg gatatcgacg tttctgtccg gtatgactgg ctctgggcat
     5161 atcagtcctc cggcattacc tatgactctg atccggaagg cttcacagcg ctggtacagc
     5221 agagtgtaga caactacaat gcttcggtaa aggaactggt ggaaaaaaag caggcagacg
     5281 gaaaacaaat ccgctttgca gatatcaaca gcgttgtcga tatgaaaacc ggcttggagg
     5341 acggcgtaca tccgaacgaa acgggttatg cctgcatggg caaatactgg tctgaacagc
     5401 tgctttctta tctgaaccag acaccgatcg aaccgacacc cggctccacc acagcaacag
     5461 tcacaaccac tacaacagag acaaccaccn nnnnnnnnnn nnnnnactgc atcttctgaa
     5521 acagagacca caactgcgga aagtacatcc attacagaaa cagaaacaag tgaaactgtt
     5581 tcagatacaa cagaaactac aacaatttcc agctctgaga gcagcacaga aactgccacc
     5641 tcccagcaac cccagccaat caagggtgat gtgacactgg atggaactgt gaatgttgca
     5701 gatgtcgttc ggctttgccg ctatc
//
LOCUS       DAWENO010000130         5718 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5464_length_5718_cov_12.723821, whole genome shotgun sequence.
ACCESSION   DAWENO010000130 DAWENO010000000
VERSION     DAWENO010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5464_length_5718_cov_12.723821"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    900..914
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1476 a         1492 c         1684 g         1051 t
ORIGIN      
        1 tgtgctatac taaagccaat ccgttgtatg acgaatacaa cagttgaaac agttaccaga
       61 atcgtgcagt tttgacttga ccaaaggcac ggcaaagtac gcagttcaat tttagcaagt
      121 cctctaacag gctcccctaa caggggagct ggcaagccgt aggcttgact gaggggtcag
      181 tctccttgta agcttacgct gacatctcag cacaaggcac gccctacggg tgccttgtta
      241 ggaaagccta tgtgaactga tgcagcattc ctttttcaag agatgcaaaa tgaatctgcg
      301 tgacggcaaa gggaggaagg aatatgggaa aatccaagaa aatcatcatc ggcacagttg
      361 ccggtgttac ggctgcggcg gtatgcgtca gcggcgttct ggtgtggcat caccacagca
      421 agagcagcag cgtagacggc gtggcatatg tcatgcccat ccgggatgtg aactcggcgg
      481 cgaatacagt ctccgactta cagttctccg gcgtggtcga accccaggag accaaggaga
      541 tcaagctgga cacctcaaag accgtttcgg agattgcggt gcaggagggc gaccatgtga
      601 acgccggcga tccgcttttc acctatgatg tggaatccat gcagctggaa ctccagcagg
      661 gcaatgtgga catcgagcgg atggaaaacg agatcgcatc caacaagcag cagatcgcac
      721 agctggagtc ggaaaaaaag aatgccagtg cggatgacaa gctgacctat accacccaga
      781 tccagtcgct gcagacggat atcgccaagt cggaatatga catcaagacc aagaagatcg
      841 agctggaaaa gctgcagaat accatcaaca acgccacagt caaggcggag atcaccgggn
      901 nnnnnnnnnn nnnncctatg gtgctgcgct cccgtgtgga cgattccagc tggaaaggca
      961 ccatttcgga gatctccacc gaggcggaca acagccagaa caacagcatg atgtacggca
     1021 gcagcgatga tatgaccaca gcgtccaagt atccttttta tgtgacactg gactccagcg
     1081 acggtctcat gctgggacag cacctgctgg tctccatgga cgatatgtcg gacgacagca
     1141 tcgacaagtc gggcatctgg ctgtattcgg actatgttct caccgatgag gacggcaaga
     1201 gctacgtctg ggcggcagac aagaaagacc gcctggaaaa acgggaagtg gaagtgggac
     1261 agaccgacga taccgcaggg gactgcgaga tcaagagcgg tctgtccgag gacgatatga
     1321 tcgctttccc gtccgactcc taccaggagg gcatgaaaac cacgaccaac cctgacgaag
     1381 ccacaccgga agatgacggc acagatgata ccgtggacga cagcagcaat gaggatattt
     1441 cctacgacaa aagcggaagc tctgtggaag aaggcgtgat cggcggagca gatgatgaag
     1501 ccggcatggc aggcgacggt gcagacggcg acaccgcaga cagcgtgatc tccggagcag
     1561 gagaagatgc cggcgacagt gttgacgaga tgccggcaga tgaagccgga gcagatacag
     1621 cggaaggcgg tgcggcagat ggtacttgat ttgcatgaca tctgcaagtc ctatttgcag
     1681 ggcaaagagc ctgtccctgt gctgcatgac atcacgctcc aggtggacga gggcgaatat
     1741 ctggcgatca tgggaccgtc cggttcggga aaatccacgc tgatgaacat tatcggtctg
     1801 ctggacaaac agaccagcgg cagctatttc tttgacgggc aggacatggc gcagtgcagc
     1861 gacaaccagc tttccgggat acgcaaccag aagatcggct ttgtgttcca gaacttcaac
     1921 ctgctgcccc gtcagtcggt attggaaaat gtggcgctgc ctctgctcta tgccggcgtg
     1981 cggaaaaaag accggctggt gcgggcaaaa gagatgctga cccgtgtggg actggaagat
     2041 cgtgtgaact tcaagcccac ccagctttcc ggcggtcaga aacagcgtgt ggcgatcgca
     2101 cgtgccatgg tgagccatcc ccggctgctg ctggcggacg aacccaccgg tgcgctggac
     2161 acccgttccg gcgagcagat catggaactg tttgcccagc tgaacgcaga aggtgtgacc
     2221 atcgtaatga ttacccacga actggaaatt gcccagcacg cccagcggat cgtaaccatc
     2281 cgtgacggca gactggaaca gggcggtttc ggagagcgcc ggacagaaca ggagggcgat
     2341 gcttcatgac aaaggtgaaa aagatcgtga ccatagcggt ttgcctggca gtagctgccg
     2401 gcggcggcac aggcggtacg cttctgtatc gctccagaca ggcgaaaaag catgaggtag
     2461 atgtgatgcc cgttgccaac atggtgcagt actattgggg cgatgatctg tcgatggatg
     2521 gtcaggtcgt ctccggcgat gtgcagaacg tgacaccgtc caacgaccag ctggtggaaa
     2581 aggtgctggt caaagagggc gaccatgtga caaagggaac gccgctgctg caatatgaca
     2641 tgacggcggt gggactggaa gtggcacaga aacagacggc gctcgccgtg gcacaggaca
     2701 acgtgcgcca ggcgaagaag gaactggaac gcatccagaa actgaaaccg tcagaagagg
     2761 cgccggtgat cccggattat cccgactatc cggacgagcc agacgaaccg gatacgccgg
     2821 acacacccgg aacgcccaag gataccctgg cggcggtgac ggcgctgaca caggcggcag
     2881 ccggcagcgg cttggagagt gacccgttcc agttcgcctg caccggaacg acggtggtca
     2941 agcaggcagt cctggaccag ctgaaagcat cgggacagtg cgctgtcttt gtggtgtaca
     3001 gcgaccaggg cgaagtgctg tacggctggc tggtgcgtgg cagccagctg accgctacgg
     3061 gagactggac actgggacag aatgttaccg tgaccgagga cggcggcgtg cagatctccg
     3121 gaaacggcgt ctggtacggt acgctccagg tgggcggtac attcgactat gacccggcag
     3181 gcggcgatga ttcgtccggc gatacaacac agccggcaga caccacaccg gggacttcgg
     3241 atgatgcagc gtctgcgccg ggaacgcaga ccacatcacc ggacaccaca gctgcaccca
     3301 ctgcacagat gcagggcgga tttgtgataa cgcctatggc ggcaatacag cccatggcat
     3361 ccgctgcatc ggacagcgac aactatgtct acacccgtgc gcagctgaaa cagaaggcgg
     3421 cagaacagca gatcgccatt cagtccgcag agatcgacga gaaaaaggcg cagatcgact
     3481 atgacggcgc agtggaaaag caggaaaagg gacaggaact ggcgaagatc gacggcgttg
     3541 tcacaaaggt ggcagagtct gtggaatccc tggagaccaa cgagccgtac ctggtagtac
     3601 agggcagcgg cggcatcatg gtacagggca atgtcagcga gatgaacctg gacaagctga
     3661 cggtcggttc ccagatcaca gtgaacgcct gggataccgg agaaatgctc accgcagagg
     3721 tgacagagat cggcacaacg ccgactgcct acaccaacca gaacggcagc gagaacccca
     3781 acaacagcac ctatccgttc caggcggcag tgaccgattc caccagcctt tccgtgggca
     3841 gctatgtgag cctcagcttt accagcgagg tggacagcag cacgttctat ctgcccatga
     3901 gctatgtgcg gcaggaaaac ggacagtact atgtgatgcg ggaatccaag gacaaaaagc
     3961 tggagcggca gaacatccag acgggaaaga tcatcgacgg cggcagcgcc attgagatcc
     4021 tgtccggcat cagccaggag gactacatct gttttcccta tggaaaatat gcgtcagagg
     4081 gtgcgctggt ccatgagacc aacggcacat cggaatatta aaggagggac agaatatgct
     4141 ggaaaacatc cgtttatcct tacagggcat tttgtcccat aaggtgcgtt ccattctcac
     4201 catgctgggc atcatcatcg gcattgcggc gatcatcgcc attgtttcca ccatcaaagg
     4261 cacaaacgag cagatcaaga acaatctgat cggtgcggga aacaacacgg tcagcgtcca
     4321 gctgtaccag ggcgacagcc cctatgactt cacctatcag tcggcgccgg acggcgtgcc
     4381 ggtcatctcc caggcagtgc gggacaacat cgccgcactg gacaatgtgg aatcggcatc
     4441 cttttaccgc acacgttcct actgcaacaa cgtatactat cagaacaccc agttcagcgg
     4501 cgccatgttc ggcatagatg ccagatactt tgacacagcc ggctatgagg tgcagcaggg
     4561 aagaggattc ctcagcaggg aatacaccga caacgccaag gcagtgatcc tggacagcac
     4621 cgccgtcaag agcctgtatt cctccgaaga tccggtggga acggtcatcg acatcaacgg
     4681 cgagccgttt acggtggtgg gggttgtcac tgaggtgaac agcttcgaac cggtcatcaa
     4741 ctccgaagag gactactgga cttatatggg aaacagcggc ggcaagatgt tcattccgaa
     4801 ccagtcctgg cccattgcgt accagtatga cgaaccggaa aactgcctgg caaaggcagt
     4861 ggacactgac agtatgccgg ctgtgggaaa aaaggtggca gaactgatga acgccaccat
     4921 ccgcaccacc agcgccggcg gcgaaaatga cagcagccag gtgaaatatg acagccagga
     4981 tctgctgaaa caggcgaagt ccctgcaaaa gctcagctct tccaccaata ccatgctgat
     5041 ctggatcgcc agcatttccc tgctggtcgg cggcatcggc gttatgaaca tcatgctggt
     5101 atctgtaaca gagcgtaccc gtgagatcgg tctgaaaaag gcgatcggtg cgaaaaaggg
     5161 aaagatcctg gcgcagttcc tgacagaggc atctgtgctg accagcatcg gcggcatcat
     5221 cggtgtgggc atcggtatcg ggctggcaca gctgatctcc aagatcgcat ccgtgccggt
     5281 ggcaatcagt tccctgtcca ttgtggtttc cgtggtattc tccatggtgg tgggcattgt
     5341 ctttggactg atcccgtcca tcaaggcatc caacctgaat cccattgacg ctctgcgcta
     5401 tgagtgacgc agtgtcattt tactttcaat ttgttcaact tgttctatga taaacagaaa
     5461 gctccggtct gcggaaaaat tgcagaccgg agctttcgca gttggtgaaa atggctgctg
     5521 tacacattgc attttctgaa aaatcccctt gacaaatggg gcgtgatagc gtataataga
     5581 aatagaaaga gcataccagt agacggtctg ctcccactgc tttagattaa aaaagattta
     5641 accgccacag gtttggagac tatgggcggt tatttctttt tattctcgtc attgcggtga
     5701 tctgcattac tattttta
//
LOCUS       DAWENO010000131         5712 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5473_length_5712_cov_11.157327, whole genome shotgun sequence.
ACCESSION   DAWENO010000131 DAWENO010000000
VERSION     DAWENO010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5473_length_5712_cov_11.157327"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1324 a         1668 c         1503 g         1217 t
ORIGIN      
        1 taagaccctc cgtggaccga gaggcggtct gatcctcacc aacgacgagg aactggcaaa
       61 gaagatcaac aaggcagtat tccccggcac acagggcggt ccgctgatgc acgtgatcgc
      121 tgcaaaggct gtctgcttcg gcgaggcgct gaaaccggaa ttccagacct acggcaagac
      181 cgttgttgca aacgcatccg ctctggctga cggtctgacc aagcgtggtt tccgtctggt
      241 atccggcggc acagacaacc atctgatgct ggttgacctg cgtcccttcg gcgacctcac
      301 cggcaaggaa ttcgagcgcc gcctggacga agtgttcatc accgtcaaca agaatgccat
      361 ccccaacgac ccgcagaagc cgtttgtcac cagcggcatc cgtgtgggta ctccggcagt
      421 caccacccgt ggtctccagc cggctgatat ggatatcatc gcacaggcaa tgtacctcac
      481 cgcatccgac ttcgagaaca aggcagaaga agtccgtgag agcgtctgcg aactgtgcga
      541 aaagtatccg ctgtacgaat aatccatgca cacacctctt gcagaccaaa tccgtccggc
      601 atccctggat gacatggtgg gacagcgcca gttgctcgcc tcgggaaaac ccctgcggcg
      661 cattatcgaa agcggcaaga ttcccaacat gatcttctac ggaccgtccg gcgtgggaaa
      721 aactacggtg gcacgcatca tcgccgacag cacctctatg acgctccaca agctgaacgg
      781 cacgtctgcc tctacggcag atatccggga cgttgtggca gaggtcggca cattctccgg
      841 catgaacggc atcctcctgt atctggacga gattcagtat ttcaacaaga aacagcagca
      901 gctgctcctg gagtacatgg aaaacggaca gatcacgctg atcgcctcca ccacggagaa
      961 cccctatttt gcggtgtaca atgcgctcat cagccgttct accgtgttcg agttcaagcc
     1021 ggtcccgccg gaggagatgg agcgtgccgt ggcacgtggg ttccgctgca tggcggagca
     1081 actgggcgaa gaaatccacc tgtcagacgg cgtctgcggc tggatcgccc gtggctgcgg
     1141 cggcgatgtc cgcaaagcca tgaacacggt ggagctgatc gttctctccg gcgaacacac
     1201 cgaaaacggc atcgtcatct ccgacgagct ggcgcaggaa ctcacccagc gcagcaatat
     1261 gcgctatgac cgggacaacg accagcacta tgacctgctg tctgcgctgc aaaagtccat
     1321 ccgtggctca gacgaaaacg cagccctgca ctatgcggca cggctgcttg cggcaaatga
     1381 tctgttgtct gtctgccggc ggctgctggt gattgccgca gaggacgtgg gtctggcata
     1441 tccccaggca attgtcatca cgaaagcctg cgtagacagc gcccttcagc tggggctgcc
     1501 cgaggcacgc attcccattg cggaggcagt cgtactcctc gccacttcgc caaagtccaa
     1561 cagcgcctac gaagcgatca atgcggcaat ggcagacgtg gaggaatacg gtgcgccgga
     1621 ctttccacgg catttgcaga acaaacactt tgacggcgca gaggcaagca tccggggaca
     1681 gcactatctc tatccccaca gctatcctaa ccactatgtc cgccagcagt atctgccgga
     1741 tattctcaaa gataaggtgt actaccgctt tggagacaac cggcaggaac aggctgcggc
     1801 agcttatcgg gaaaagatcc tccgggagga caagacgggc ggcaaataac ccctgccttc
     1861 cgggaaagcg ccgccagggc tgcatcatac aggctggacg gtcgaatctc ctgctgcacc
     1921 agcgcattgg caaagcggat ggtctgttcc agatcggacg aaaagtcggc gatgctgtat
     1981 cgttcgccgg tgcgcaggtc ctccagcgtc acaccgtaaa acggctctga gacgctctgc
     2041 cggatcacgc tcccggcggt gacacgatag atcacacggt tgctgccgta cacttccata
     2101 tagacgatgg gttcggacag ccggggctgg aggtcggatc tctttctggt tttcagtttc
     2161 attgtgatac tcctgttctt ttgcgagcga aacggctctg tgatatctct ttctgtctac
     2221 cagtatagca ctttttcccg aaaatgaaaa ggctgaaaaa aggcggatct tgtcgagatt
     2281 ccagactttt ccacgaaaca atgcaaagct gacacacgaa aaacgcattg cgttaaaatg
     2341 tggtgaagtt cctgtaaaat ccacaagttt ccgccgattt cagtcaggaa tctttggcgg
     2401 aaatgcgact agacgaaagc atatgatgtt gctataatag agaaacgtac ttttctgaaa
     2461 ggggattctc tgttatgaca aaggatatga cagccggaaa gccgctgggg ttgattctcc
     2521 gcttttgcgg accgatggtt ttcggcaaca ttttacagca gctctacagc atggtggaca
     2581 ccgtcatcgt cgggcgctat ctgggtgcca gtgcgctggc tggcgttggt gcaacaggcg
     2641 ccatcagctt tctggtggtg ggatttgcca ccggcatctg cacgggactt tccattcctg
     2701 tggcgcaggc gttcggcgca ggcgactatc acagtatgcg gaagaacatc gccaactcct
     2761 actatctggg agcgctcatc ggcgttctgc tgaccatcct caccatgatc ggcacaggca
     2821 cgatcctgca ctggatgaaa acaccggatg acatctatca gtattcttac gactacatca
     2881 ttgtcatttt cggcggcatt ctcacctcgg tgctgtacaa cagcctggct gcgatcctgc
     2941 gtgccgtggg cgacagcaaa tccccgatcc tgttcctggc ggtctcgtcc gtgctgaaca
     3001 tcatcctgga tctggttttc ctggtgggga tgcagtccgg cgttgccggc gcaggctatg
     3061 ccaccgtgat ctcacagggc atctccggca tcctctgcct gatctatctg tataaaaagt
     3121 ttcacatcat gcgtttccag cacggcgaat gcaagccgga gctgaaacgc tgcggacggc
     3181 tgctctccat cggtctgccc atggcgctgc aattttccat caccgccatc ggctccatca
     3241 ttttgcagac ctccgtcaac agcctggggt ctctggcagt taccagcgtc acagcggcgc
     3301 agaagatctg tgccattgtc atgggacctc tggaatctct gggtatcacc atggcgacat
     3361 tctgcggtca gaacctgggt gcaaaaaagt tctaccgcat ccgggacggc atccgtctca
     3421 gtatgtatgt ctccatgtgc tactgcgctt tctgcatcgc ctttctctgg atcctgggaa
     3481 aatacacggc gtatctgttc ctggatccgt ctgagaccga ggtcatccgc ctggcggtgc
     3541 agtatatgcg ctggaatgcc gtgttctatc cggtactcgg cgttctcttt atcctgcgca
     3601 attccctcca gggcatggga tacagtctca tgcccatgat ggctggcgtc agcgagctgg
     3661 ccgcccgttc tctggtctgc ttcggctttg tccccacctt cggctatatg gctgccatta
     3721 tggcaagccc ggtggcatgg atctttgccg atgtgctgct catcaccgtc tactttgtgg
     3781 atatgcgcaa actcaagcgc cgccttgttc ctgcggaggc agccatggcg gcttagcaaa
     3841 agcgtgatta cgctgttttc aagaaaggat cgctctatga aaaaaatgat ctctctggtc
     3901 agtgccgctg tcatgacggc atcgctcctg ctgccgcaga ccatggcatt ctccgccgct
     3961 gcgcaggagg acggcgatgc gctgatcgat gccgcataca gtgaaaatgc actgacagca
     4021 accggtacgg aaaataccct gccctatcag ctgcgctttg ttccggaacg gaacttcgtc
     4081 accgcagaag aagtggcagc cggcgacgtt gtcatcccgg catcggtcta catcaccggc
     4141 agcaccgcca acaccttcgg ctctgccatg gtgaagtaca acgcctatca caacggcgaa
     4201 aagaccggcg atgtttactt caaggacatc accaccggcg acaacgccca caaaaactcg
     4261 gagaaaaaga cttataacag cagcgtcggc tcgttcacca cagaccaggt tccctactgt
     4321 ttcggctcac tgatcggcaa gaacaacacc tataccaaca actcccccat gttcaccgcc
     4381 agagaatacg cctgtgaccc gatcttcggc tctacactct acagtgccgg cagcggcaag
     4441 atcacattct ctgcttcgtt ctataccagc gcagaagacc gtaaaaacgg caaggcaaag
     4501 acgaccaagg acttcccctg tgacgtcacc gttgatgcag acggcaacgg cgtatactcc
     4561 tacgagtata tcgaccagac cacgttccag accgtcacca agaccgcatc cattccccgg
     4621 tacaatccgg acaccccggc gggcgaacgg ctgccggaca cctgcagcag cgtgatgtgg
     4681 gtggctggta cttcacagtt gaaagacggc gcatccttcc tgggcaatgc ctctgacgag
     4741 tttccgctgt tccaggtgaa cgtggtaatc cagcagggta ccaagcccgg cgtatatgac
     4801 atcaagttcc tggaccgtca gtctgcggca tccggcgaga caccctgcca gctgaccagc
     4861 agcagaagca aggaatttcc caccgaactg gtcggcactt ccatcgctgt cggcgtggac
     4921 agcctggtgg tcacagatac cgaacaggac gacgtgaagt tctacaccga tgataacacc
     4981 aaggcgatcc gtgccacaga ctttgccaca aagatcaccg cagatgtgac ctatgcagat
     5041 tccaccacgg aaaccggcgt ggacatcacc ggtctggtgg actgctacgg cgcaaccccc
     5101 aagaccctgt acgacaagca ggcacaggac ggtttcttca tctctgagaa tatgcccctg
     5161 tactgcaacg gcagcctgct gaagtggaaa gacaccggca ataacgtaac ccagaacatt
     5221 atggtaggca aacgtggcga catcaaccac gacagcaagg tagacagcca ggatctgttc
     5281 tatctcatgt actatatcgc actgaagggc gtgggcagcg atgatgccaa gctctatgac
     5341 ggcaaatcct ctaaccaata catggaatcc ttcctcatgt atctgatgga cttcgacaca
     5401 tggggcatga ccagtgacgc agcgctgaac agcacggata tgtattatct gatgctgtac
     5461 atcgcaaaca aagcggtagg caacaacgtc acctggaatg cgtacgtgca gtaatcacgc
     5521 agtttttaac tgctttaatt tcactaaaag ggtaatcccc ctgtcagctt atgctgacat
     5581 cccccttttc aagggggact ttctaaaaaa ttgatttgtg tgattcacac aacaaccaaa
     5641 aggcgctgac ccgttcgcaa tgggtcagcg cctttttttc gggatctctc ccgttttttt
     5701 ccgaaaaagt aa
//
LOCUS       DAWENO010000132         5708 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5479_length_5708_cov_9.421015, whole genome shotgun sequence.
ACCESSION   DAWENO010000132 DAWENO010000000
VERSION     DAWENO010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_5708_cov_9.421015"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    245..254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1661..1670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1315 a         1590 c         1519 g         1264 t
ORIGIN      
        1 taccataaag gcgtaggaaa tgcaactgat tttttagtga aaattacgtg atatgggttt
       61 atggaacaaa ggatttcgca gcacaatctg caattttcac agagggacag cgtcggcgca
      121 tcccacgaca ttccggaaaa aatcccccgg attttctgaa aaatctgcga atccggttgc
      181 agtctgccga aaaatatgat atactgaaaa agataacacc ttgtcattgt agagacaagc
      241 cgaannnnnn nnnnacaccc aacgaaaaga ggtaatgccc gtcatgaatc aaaagcaaga
      301 tatgatagat accgaagaga ccatgctccc cgagccggat gccgcactgc tcccctatat
      361 ggaaaggctc tatcccctca cctttgccct caagcagcac aaaccgtgga aaaccatcta
      421 cgagtacgag gcgttcgccg tccgtctgcc cgacggcacg cctgcctgct gtctgctgac
      481 cggcatggag ggagacttta tctcgctgtc tgtctattcc ggcgaaacgg gctggaacaa
      541 catccacaag ttctgggaaa caccggacga accggacaaa acacaggaaa attaccagga
      601 actgatgatg tcccagtcct gtctccaggt cgtattctgc tgcaaggatg acctggcagc
      661 tgccgaggtg gaaagtctcc gccgctatgc caaggaaaac ggcatcacct tccgtggaaa
      721 gaacgcctac ttctgtttcc gcagcgccaa gccctatcat gccacctggt tcctggaaaa
      781 tgagacgcag gcggacatcc tggagacggc gctccacgct gcactagaca ttgccgcaca
      841 gctggaacag ggcaagacca aacaggaact ggggctgctc ccctattccg ctgtcctgcc
      901 cctgtatgtt cccactgcgg acggcgcagt ctgggaaacg ctggaaattc cggaagagac
      961 agaggtcact ctggcagagc cgcacctgga cgctgccatg cagcagaaac tgcgccggat
     1021 gaaaaaaggc ggcggcaagt gggagtgcca gctgttcccg ttcccggacc cggttcgtgc
     1081 cagagagggc gcaccgtttt tcccgttcct catgctgatc ctggacacca agcgggacca
     1141 gctcctgccg cagcagcctt tgcaatacaa cgatgccgtc ctccagcagg cactggacgg
     1201 ctttgtccag gcagtcatcg actacggccg ctgcccgaag agcatcgccg tctgcgacaa
     1261 gcgcaccgag gcggcgctct ccggaatgtg ccgcagctgc gggatcctcc tgctgcgcac
     1321 cacacgcttt gccatgctgg acgaggcgaa agcgtcgttt ctggatgacg tgaacccaaa
     1381 ggaagaaggc ggcgcaacgc tggaagaggt cgtggctctg ctggaacaga tgccggacgg
     1441 cgaactggca aatctcccga aagaaagcat ccagatgatg ctggaagaga agatcccgga
     1501 aaagctgcgc aagcggctga aaactgcact ggaggcgaag taatcctatg agcgaaacca
     1561 tttaccgagt tctgcacatc tttgaaagcg actacggctg tgaggaacac agcgaaccga
     1621 tggtagacgt agttctggaa tcgccggacg gcagacgcag nnnnnnnnnn attccctatt
     1681 ccgatgcccg gctgtatgcc ctggacatct tcgagggtga cagcgtgacc ctgcaccagg
     1741 gacagctgaa aaaggtgcat ttccagggag aaaacgcatg accgaagtcg aagtacagca
     1801 gcgtgccaag cgatatctgg actgcctggt acaaggcatc aatcccctgg acggcacagt
     1861 catcccggaa ggagacaccg tccggcaggt acgcattgcc aggtgcctgc aatatgtctc
     1921 cggcatcctg gaacaggtga tcgagaacca cggcgaagta cagcgtgtca agaaaaagcg
     1981 ttccgaacgc ctgccgtttt cgctgacgcc ggaacagctg ctctctctga aaccagagcc
     2041 acagccagta agcatttctg agatcgtaca gaagtgcaat gccatgatcg atgaatacac
     2101 catgtgcagg ctgaaaacca ccaccctcac caaatggctg gtgcgtgccg gttttctgga
     2161 agagatctcc gtcggcgaga accgtcaccg gaaaatcccc actgcaaacg gcactgccct
     2221 gggcattgcg gagaaaaccg tacagagctt tgatcgtact tatacggtag tgacatattc
     2281 gtcccgtgcc caggagtttc tcttcgagaa tctcgaaatg atgctggaag aaaacgacta
     2341 gcacattacc attataataa gacaaaaacc gcctccggcc gggaatcact cccgatcaga
     2401 gacggtttca tttattgatc ttttttggtc tgttccgcag aaactgtttt cgcctgttcc
     2461 tttgccgctt tcagcttttt caagctgggc tgcatgacca gaaaggcagt gacaccgcag
     2521 gatacggtga ataccaccag aacgatcacg atctgctttg tctttccgct gtcctttgca
     2581 ttgtccgagg actgcgactc cgccgcagtg gtctgcggtg cggcagtttc agccgccgct
     2641 gccggaatcg tccagagact gcccagcaga aacagtgttc ccagcagaac gcaaaggcgt
     2701 cttttccaac gctgccgcat ggatttagcc cagtgtggac ggcattgtcg cagacgacat
     2761 tgctgcgctg ttgtgttcca gatatgcgtc attcttgacg atcttgacgt tgttgtaaac
     2821 gtccatacca gtaccagccg gaatcagctt accgataata acattttcct tcagaccata
     2881 cagatagtcg ctcttgccac ggattgcggc atcggtcagc gcctttgtgg tctcctggaa
     2941 ggatgcagca gacaggaagc tgtcggagct ggaggatgcc ttggtaatac cctggagcat
     3001 cggctctacg gtaaccagag aaacctcggt ctcaccgttg tcgatgcgct cctggagtgc
     3061 atcgttgact tccatgatct tgcggcggtc gaccattgta cccgggagca gatagctgct
     3121 gccggaatcg tctaccttca ccttgcggag catctggcgt acaataactt cgatgtgctt
     3181 atcgttgata tctacaccct ggaggcggta aaccttctgt acttcgctga tgatataatc
     3241 ctgtactgcc tgaacaccca gaatattcag gatgtcgccc gggaatacgt taccctctgt
     3301 catgcgggta cccttcttga tgtattcgcc ttcacgaaca cggatcttga agttatagtg
     3361 gatcggataa gattccgact cgccggtctc atcgttggta acgatgatgg tacgtgcggt
     3421 cttgatctct tcgatgtgta ccacgccgtc gatgcgggac agaacggatg cgttcttcgg
     3481 gtgacggctc tcgaacagtt cctcgacacg aggcagaccc tgtgtgatat cttctgccga
     3541 tgcgataccg ccggtatgga aggtacgcat ggtcagctgt gtacccggct caccgatgga
     3601 ctgtgcggcg ataacgccga ctgcctcacc gacagatacc aggttgccgt gtgccaggtt
     3661 tgcgccatag cactttgcgc agacaccctg tcttgcacgg cacatgagga cggaacgaat
     3721 cttgatacgc tctacgccgc agtccacaac cttctgtgcg tctgcctcgg tgagcatctt
     3781 gtttttggag atgatgagtt cgccggtctg cggatcacgc agatcgtcta ccagataacg
     3841 gccgaccaga cgctcggaca gcttttcgat cagctcgttg ccattgcgga tctcgtagat
     3901 ctccagaccg tcggttgcgc cgcagtcctg ttcccggatg atgacatcct gagaaacgtc
     3961 taccagacga cgggtcagat aaccagagtc ggcggtacgc agtgccgtat ctgccagacc
     4021 cttacgggcg ccacgggagg agatgaagta ttccaggata ttcagaccct cacgatagtt
     4081 ggaacgaatc ggcatttcga tggtcgcacc ggaagtattg gcgatcagac cacgcatacc
     4141 agccagctga cgaatctgag agatactacc acgggcaccg gagtccgcca tcatgaagat
     4201 ggggttgtac cgatccagac cgttctgcag tgcggtggta acgtcgttgg ttgccttgtt
     4261 ccagatctcg atgaaccgct tggacttttc cgcagcggag atataaccat actcgtattc
     4321 cgaggtgatc tcatcgacct gtgcatctgc ggcgttgacg atatcccatt tttccttcgg
     4381 gatgatggcg tcgcagacag caacggtgat ggctgccttg gtggaatact tgaatccggt
     4441 tgccttgatc ttatccagca cttccgaagt ggtggtagtg ccgtggatct tgatgcagcg
     4501 gtcaatgatc tgacccagct gctttttgcc gaccaggaat gtgatttcca gatcgaactg
     4561 cttgtcggaa ttggtacggt ctacataacc cagattctgg gggatatttt cgttgaagat
     4621 cagacggccg acggtgcagt cgatgatctt ggatacccgc ttttcgccga tatccttggt
     4681 gatgcggact ttgatcttcg catggatgga aacgtcgcct tcctgatatg ccatcagtgc
     4741 ctcgtttaca tcacagaata ccttgccctc gcccttttcg ccgtcttttt ccatggtcag
     4801 atagtacgaa cccagaacca tatcctgtgt cggtacagct accggacgtc cgtcagacgg
     4861 tttcagcagg ttgttggcag ccagcatcag gaaacgtgcc tcagcctgtg cctcagcgga
     4921 cagcggcaga tgcactgcca tctggtcgcc gtcgaagtcg gcgttgaatg cggagcatac
     4981 cagcggatgc agtttcagcg cacggccctc taccagaacc ggctcgaatg cctggatacc
     5041 cagacggtgc agcgtaggcg cacggttcag cagaaccgga tgatcgtgga tgacattttc
     5101 cagcgcatcc catacttccg cagacgcacg gtctaccatc tttcttgcgc tcttgatgtt
     5161 tgcaatgacc ttggtgtcca ccagacgttt cagcacgaac ggcttgaaca gttccagcgc
     5221 catttccttc ggcagaccgc actgatacat tttcagctcc ggtccgacaa cgattacaga
     5281 acgaccggaa tagtcaacac gcttacccag caggttctga cggaaacgtc cctgcttacc
     5341 tttcagcata tccgacaggg acttcagcgc acggttgttc ggaccggtga ccggtctgcc
     5401 gtgtctgccg ttgtcgataa gggcatcgac tgcctcctgg agcatacgct tttcattgcg
     5461 gacaatgatc tccggtgcat ccagttccag catacgtttc agacgattgt tacggttgat
     5521 gacccggcga tacagatcgt tcaggtcaga tgtggcatat ctgccgccgt ccagcatgac
     5581 catcgggcgc agatccggcg gaatgaccgg aacaacgtcc ataatcatcc attccggacg
     5641 gttgccggac tgacggaatg cctctacgat ctccaggcgt ttcagcagct tgacacgctt
     5701 ctgaccgg
//
LOCUS       DAWENO010000133         5592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5590_length_5592_cov_10.240383, whole genome shotgun sequence.
ACCESSION   DAWENO010000133 DAWENO010000000
VERSION     DAWENO010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5590_length_5592_cov_10.240383"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1254 a         1494 c         1578 g         1266 t
ORIGIN      
        1 aaggtaattt caaccgtact ggcaggtgtt ctggcattgt ccgcacttcc catctctgcc
       61 atgagtgccg gcgctgaggg agcatatgcg ccgggcgatg tggatatgga tggctttgtg
      121 accgcacatg atgcggctgt tgtcagccgt gcactctatg tggacaacaa tctgctgacc
      181 agcgagcaga aaaagcttgc cgatgtcaac ggagatggaa aaatcacaca ggaagatgcc
      241 gactggattc atgaaaacca ggtgtacaac ctgggacagc tggacgaatc gtcagatcac
      301 ctggattcta ccggaatctt ctaccagttc tactatgtgg caagaaaggg cgccggcgtt
      361 ccttttgaag tagtagataa atcagaaaaa cttccggaaa accttctggg ttcttcgttg
      421 gggaaggatt tggacaacag caaaatgccg cagcttttct tcaacctgct ggatgtggat
      481 ggtgatggaa gtatcacagt aaatgatatt tacgcatcgg tattctcctg cgcactgtgc
      541 ggtgcaggct tgacaaaaga aaacaatttt gtacagggtc ggtatgactt gaatgcagat
      601 ttgatcaatg tgatctgcga agaagatgaa tccggcagtc tggcatattc ccgagatcat
      661 actgttatgc cggatgatat aaaagcaatg ttaaacgtgg attaagtttt atcttgtccc
      721 cgttttaagt gtgaaaagag ccgcaggcag aaacgcctgc ggctttttca tttgcgtaaa
      781 accgtgaccg ccgcagacag acacaaacaa aacgactcct caccaacttt ccggtcagga
      841 cagccgctaa atctggtatg caaaaaaaac gcaaaactgg taattgtaat agtgccaatt
      901 ctgtgatatc ctaaaggaag tagctttgga aattccagag caaaccgaaa ttgatggagg
      961 aatcaccatg ggaaatcgtt atgaactgaa caaaaacctg gcgcagatgc tcaagggcgg
     1021 cgtcattatg gacgtaacta caccggaaca ggcaaagatc gcagaggcag ccggcgcttg
     1081 tgctgttatg gcactggagc gcatcccggc agacatccgt gcagccggcg gcgtatcccg
     1141 tatgagcgac ccgaaaatga tccgtggcat ccaggaggct gtcagcatcc cggtcatggc
     1201 aaagtgccgt atcggtcact tcgcagaggc acagatcctg caggcgatcg agatcgatta
     1261 catcgacgag agcgaagtgc tgtctccggc ggatgacaag tatcacatcg acaagaccaa
     1321 gttcgacgtg ccgtttgtct gcggcgcaaa ggatctgggc gaggcactgc gccgcatcaa
     1381 cgagggcgca tccatgatcc gcaccaaggg cgaaccgggc accggtgacg tggtacaggc
     1441 tgtacgccat atgcgcatga tgcaggcaga gatccgccgt ctccagtcta tggcagagga
     1501 cgagctgttc gacgctgcca aggaactgcg tgtaccgtat gatctggtac agtatgttca
     1561 cgacaacggc aagttgccgg tagtcaactt tgcagcaggc ggcgtagcaa ctccggcaga
     1621 cgcagcactg atgatgcagc tgggcgctga gggcgtgttc gtaggctccg gtatcttcaa
     1681 gtccggcgat ccggcaaagc gtgccgcagc gatcgtaaag gctgtcacca acttccagga
     1741 tgccaagatg ctggcagagc tttccaccga cctgggcgaa gcaatggtcg gcatcaacga
     1801 acaggagatc tccctgctca tggcggagcg tggtaagtaa tgcgcatcgg tattctggca
     1861 ctgcagggtg cattcgcaga acacagcgcc attttgcaaa agctgggtgc agacagtttt
     1921 gaaatccggc aaaagcgtga tctggcgcag cctatggacg ggcttatcat tcccggcggc
     1981 gaaagcaccg tcatgggaaa actgctgaaa gagctggact gttatgacga agtgcgcagc
     2041 cgcatcgaaa acggactgcc ggtattcggc acctgcgccg gtttgatcct gctggcgaaa
     2101 gaggcagagg gcggaaaggt acagctggga accatggaca tcgtggcccg gcgcaacgcc
     2161 tacggcagac agctgggcag cttttccacc accgcagaga tgaagggcgt gggcgaagtc
     2221 cccatggtat tcatccgtgc gccctatatc acttccgttt ccggcagcac agaagttctg
     2281 gcagaagtgg acggcaagat cgtggcagcc agagagggca gccagctggt tacggcgttc
     2341 catccggaac tgacggagga tacccgtgtg catggctatt tcctgcacat ggtggaagag
     2401 gcaaaggcgt gacctgctcc aaacgataac ataaaatgag aaaaccgcat cgcttttcgg
     2461 aaacatccga tgaacgatgc ggcttttttg ttttggtatc aaccagtttt ctctgccgct
     2521 ttcagttgcc gcagatagcg gttcagaaag gcgtaaaagc tgcacactgc ctcctgcact
     2581 ctggcgtggc ggtggagctg caggatgatg gcacgctgac aggtcggctc ggcaatgggc
     2641 atcagccgaa tctgttccat gctgtctgta ctccaggtat agtgcggcca gaatcccacg
     2701 ccaagaccgc tggcgatcag gttctttacc gtgtcggggc tgtcgctctc gtagatgatg
     2761 tgcggcacaa agcctgcctg catacaatag cggtcacaga tggtgcggat gctccgggaa
     2821 ccggacaggc tgataaagtc ctggtgggcg aagtctttca gccggacgct ctcttcgctg
     2881 gcatagggcg aatctgccgg cactgccaga aagatgcgtt ccttgaacag ctcatagcgg
     2941 tctttgttgc cggcaggaat ctggaaattc tcccgtgtga aaaccgtgat gtccgcatcc
     3001 tcgcactggg agttctggaa aagctgaaag ttcagcccgt cgtgatcttt cttataggag
     3061 atcagcgcct gggtcaccag cgtcgatgcc gccagcacgt tcagacggat ggtggtctcc
     3121 cgttcctgcg ccagctgctg gagctggttc ggaatctggt gcagcgtctt taacacagga
     3181 gtcagcgcct cctggagtgc gacgccgtat tccgtgagcg caatgtttct gccgctgtga
     3241 tagaacaagg gtacgcccag ttccttttcc agccggcgga tgctctgggt cagagacggc
     3301 tgtgcgatgt gcagctgctc tgcggtacgt gtcatatgct ggttctgtgc caccgcatag
     3361 aaatattcca gctgcaaaag ttccatgctt atttccgcac ggtgtcgcag cttgtgcctg
     3421 cgccggcaag cttttgcagc tttacagcgc accagccgga tgcctcacgg cgctccagga
     3481 cggcgaagtc ataggcagtc acggtatcca gcacctcgtc tgcacgctct gtgatgatgc
     3541 cggacagaat gaggatgccg ttgtctttca gaaaaccggg gaaaagaggc gccatgccct
     3601 tgatgacatc tgccacgata ttggcagtta tcacgtcata gccgctgccg atctcctgcc
     3661 gcagtgtctc gtccgaaatg atgttgccgc aggaaatggt atagcgctcc ggatccaggt
     3721 ggttttcggc ggcattttcc ctggcgatgc gtgtagcgtt ttcctcaatg tccacggcgt
     3781 atgcctcttt cgcacccagg agcattcccc cgatgaacag gatgccgctg ccgcagccca
     3841 gatccagcag gcgggtggaa tcgggaacga tctcgttttc catcagctcc aggcacagcc
     3901 gggtggtgtg gtgctgtccg gtgccgaagc tgctcgccgg gtcgatctcc aggatcgttc
     3961 tgccctctgc cgcagcggtg ggttcttccc agctgggctt gatgtacagc tttttcccca
     4021 ccgggaaagg cttgaagtac tgtttccagt tgttcgccca gtcctcttcc cggatgccgg
     4081 agcagactgc ctccagtctg ccgtactgtc cttcggtgtc ctcggctttc atctgcgcca
     4141 gcatcgcacg gagtgccgcc agcgtctctg cgccctgggc atcctccggc agatagacgg
     4201 tgacgcaggt ctcacagctg gcaagctcca tgagaccgtc ctccagatag tcccacttgc
     4261 cgtccttttg ttccaggaat tcctggaagt cctcagcgtc ctgtaccatg cagccacgga
     4321 tgcccagatc ctggagccgc acgctcagca ggtcaatggc aggggtggcg gtgtaaatat
     4381 ctacttgtgt ccaattcatg cgagttctcc tttttgttcc tgccgcttgc ggacattttc
     4441 ttcctggatc tgtctgccct cggcgataag atattgcagc cgttcttcgt cacgctgttt
     4501 cagcgcatcc cgatagtcgg tcaggttttc gatgagcaga tccaggtggt acagcaggtt
     4561 gtcccggttg tccaggaaca gcagcgccca catggactca tccatggtgg caacacgggt
     4621 catatcctga aagctgccgc cggaaaagga aagggcatcg tccatgcaga aatccttgac
     4681 ataggagctg gaaaccacgt gcgccagctg agaggtgtag gcgatcatgt tgtcgtgcca
     4741 catcggggtc gccgtcacga tcttgccgaa tcccatctgc tttgccagtt ccttgacggt
     4801 atccagcgcc gcctcgtctg tgccgtccac aggcgttacg atcagattgg cattctgaaa
     4861 caggttgggg atgctggcat aatagccgtt tttctccctg cctgccatgg ggtgtgttcc
     4921 cacatagcgc acgccggctt tctggcagcg gtacgtgaga tcacgcacca tgcgcccttt
     4981 caagccgcag gtgtccacca ggatcgtccc taccgggatc ttaggcagcg cctcctgtac
     5041 catctgcaca gcgatgcggt gatgcaggca gagaatgatc atatcgtatg tgccgtctgt
     5101 catggtgcct gcgccgtcga ttgcgccgtc tgccgcagca tcctccagga ctttgggctg
     5161 tatgtcacag ccataaacgg catgggatgt ggcggctttg atggatttgc agatcgaacc
     5221 gccgatgagt cccatgccga ctactaagat tcgcatgata cgttcctcca taatctgttt
     5281 ggtataatag tattgtagca cgaatcgggg aaaacgtcaa ggcggcggct tgaatgcggc
     5341 gcaaattccc ccgttccctt tcggcacagg gcgcaaaaaa gccgcagacg ttgtgcctgc
     5401 ggctcttgaa gttgcattca gttcaaaaag cacttactta accaaatcgc taaggtcatc
     5461 cttgacaaga acagcatctt tcggtgttcc gtaaattttt gcaaattctg tgatatcata
     5521 atcatatcga ccgattgcaa agtaacggct caaatcaaaa cccgcacctt tatgtccgat
     5581 ataagcacct aa
//
LOCUS       DAWENO010000134         5565 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5625_length_5565_cov_8.028857, whole genome shotgun sequence.
ACCESSION   DAWENO010000134 DAWENO010000000
VERSION     DAWENO010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_5565_cov_8.028857"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1268 a         1388 c         1554 g         1355 t
ORIGIN      
        1 ctgcgtacaa gtgcatggga ttatgacagc agaacgcacc cgaaccgccg caaccctgcc
       61 cgcacttgaa aaaaacaatc taaaatccaa aaaaaatttc aaaaaactat tccaaaatga
      121 agaaaaatgt gctataatag tatcaaagcc gtccaaccgc acagcgattt gcggcaaaac
      181 tatcaaaaaa gaggtttttg acatggaaat caaaaaacaa ctgacgactt gtccgaaaga
      241 aaaacccaag gacgagagca agctgggatt cggtcatatc ttcactgacc atatgttggt
      301 aatgccctat gatgagggac agggctggca tgatccggta attatgccgt acggcgacat
      361 taccctcagc ccgtctgcaa tgtgcctgca ctacggtcag actgttttcg agggtctgaa
      421 ggcttatcgc cgtgctgacg gcggtgtaca gctgttccgc ccggacgaaa acttcaagcg
      481 tctgaaccgc tccaacgagc gtctggttat tccggaactg ccggaagagc tgggtctcca
      541 gtgcctgaag gaactcatct ctgtggaaaa ggactgggtt ccccacacag agggcgcatc
      601 cctgtacatt cgtccgttta tttttgcagt ggatccgttc ctgggcgtac acccgggcga
      661 acactatctg ttcatgatta ttctgtctcc gtccggtccg tactatgctt ccggtctgga
      721 tccggtcaat atctatgtgg aaaacaagta tgtccgcacc gttcgtggcg gtatgggcta
      781 tgcaaagacc ggcggcaact atgcggcatc tctgcacagc caggctgatg cctatgacca
      841 gggttattcc caggttctgt ggctggacgg cgtagagcag aagtacatcg aagaagtcgg
      901 tgctatgaac atcgctttcg ttatcgacgg cgaagttgta acaccgtctc tggaaaccgg
      961 ctctatcctg tccggtatca ccagaaagtc tgcactggaa ctgtgccgct ccaagggcat
     1021 tccggtttcg gaacgccgta tcaccatcca ggagatcgca gatgcttatg acgcaggcaa
     1081 gctgtccgaa gtattcggta ccggtactgc cgcagttatc tccccggtag gtcacctcaa
     1141 gtggggcgat aaggtcatga ccatcaacaa caacaagatc ggtccggttt ctcagatgat
     1201 ctacgatacc ctgaccggca tccagtgggg caagatcccg gatgaattcg gctggatcgt
     1261 tccgattgac taagcagacg gcagaaatgg aagtacattt cgaggatttg aaagaagagg
     1321 atctcagaga ggtttacgac ctgtgcatgg aaacattcca ggagtcgttt gcttttgaag
     1381 agatcgagga gacctttcag ctgtgcagga atgatgcgca ctatcggttt ctggtcggaa
     1441 aatcccaggg gaaggtcgtg gcgtatacct cgatcaacat tttccacaac ctgtttgacg
     1501 gccgccgtcc catcatgacc ctttggtacg tctgcgtcag caaggaagtg cggcgcaaag
     1561 gcgtgggacg tctgctgttc cgggagatcg aaaagatcgc aagggaaaac gactgcgaga
     1621 tcatttattt tacctgtttg caggaaaata ccggggcgca gaatttctat cggtcgctgg
     1681 gctatacaga tgaaaaggaa aaggcatttg taaagtatct ggattgagaa aaggcatcga
     1741 acattctcct ttccaatttc tccgcagcac tgcctgttgt ttgaaataac atgataacaa
     1801 aaaagatagt ttctcacagc gtcaagcatg agaaactatc tttttttatg ggcgcagtgc
     1861 cgcaagcagg tggttccagg tggcggcgtc tactgtgccg ctgggcggca aaggcgtcag
     1921 ctcctggaac tgctgcaccg catttttggt ggcagtgtcg taaacgccgc tgctctgtac
     1981 ctgcggaacg gcaggaaacg cactgtggat cgcctgtaga acgccctgta tcacaaagac
     2041 ggcgctgccc ttgtcgcccg gggcataggc agtgatgccg gtggggaaca gccggagatg
     2101 cagtacggtt ggacgcactt cctgtcgata tgcctgtgca atggcgttcc aggtggcggt
     2161 gtcggtctcg ccggtgggcg gcagctggtt ctgctgctgg aacagccgga cagcgtttgc
     2221 cgtattttcg ccgtataccc cgtccggaat gagatgcggc agctggctct ggtgagaaat
     2281 gccgtggaga tatgcctgca attccaggat atgctgccgt ttttgttcgt ctgtataagc
     2341 catgggatgc ctccttaggt gatgatatag cccggtgcct ggaaaggctg tttgtcataa
     2401 ccgtatttta agtcggagta caggctggca atggcgttcc agacggcggt gccgaccaca
     2461 ccggttttcg gatagccgaa aagttcctgg aatgccatta cggcggattt tgtcatggga
     2521 ccgaaatagc cggtggcgtt gacggcagga atctccggat aggtgccgtg gatataggtg
     2581 agatactgct gcatcagacg gacatactcg ctggtagtgc cctcccgcag aaccacgccg
     2641 gggtagagca ccacgtcatt gaccgaggtg tccagcggta tcgattccac aatgccggtg
     2701 taggcatcat agagggcgtt ccatgtggaa aaatccacca cgcccgtcac cggaagcccg
     2761 aacgcctgct ggagggattt gacggaagtt tccgtctgtt ctccgaagta gccggtaatt
     2821 tccaccggct gtatggcggc atagtaggct cccaccactg caagatgata ctggagggca
     2881 cggatgttct tgccctgcat tccgatgtgg agatcttcgg tataggtctg ttcgatctcc
     2941 tgctgggtga tgccctcgga gttcagctcc gccagatttt ttacgctggt aaaaatgtag
     3001 gagatctgat accaggtcgc ctcatttacc acgccggtcg ccggcagtcc gaagatctgc
     3061 tgaaaggttg tgacggcatc ctgggtgata tagtcgaaat cgcctgctgc atcgccgatt
     3121 ttggggatgg caggatagtt ccgggaaatg cggttcagct gtacctggat ggtgcgcacc
     3181 ggatcgccgg aaagtcccag agacagatca tagcccggat aagagggcgt gttggtcaga
     3241 acggggacgt ttgtcacgat gtcgatatcg ttgccgtaat agctggtgag gatttcatag
     3301 ggcgtcatgc cctgctgtgc cagggacact gtaccccact gggaaagtcc gtcgcaggtc
     3361 acagtcgtgc cgttgcagaa tgccgtgaac agcggttcta cattgccctg ccgccggaca
     3421 taggagttga acagttcatc caccagctgg ctgatatttt cgaaaacctc tctgccgtag
     3481 atgtacttct ggtcaaactg ggtggaactg gtgatatcga aaggataccc acgggagcga
     3541 taccattccg tgtacacccg gttgagcgca aagctgatga tggcgtagat gttggcacgg
     3601 atggcatttt ccggccaggt ggggtagatc tcgctggaga ctacgttctt gatgtaatcc
     3661 ggaaaggcga ctgtgacgtt gtccgccgcc gcatccggtc tgcccaggtg gaccgtaatg
     3721 tctctgggaa taaagggttc tccgactggc ataccggtat ccctccttta cagattgggt
     3781 tcgttgttct gatagacgat cggctccggc gattcctgca aatagctggg caggggaatc
     3841 atggcgaaga tctgacggga agtcgttccg gcaaagaccg gcacagcctc gctgatctgc
     3901 tgataatagc cgtctgcata aacgctgaca tcataggtgc tgtagggctt tcgggtctcg
     3961 ctgctgtcca gcgaaaggga tgccgccggg acgggcagcg aaatcttttc cgtttcgccg
     4021 ctctcgttgg tgacggcggt gtgccgcaga tgcagctgct gttccgtgcc gtttgtcacc
     4081 aggacggaca cgccgctgat ggcacgggcg tttccggcac tgcgtgtgat gatctggagc
     4141 cagccgatgg caggctgttc cggggatgtc tcgtcaattt ccagctgcgg cggcggaacg
     4201 gtttctgccg gcggcgctgt ctcttccgtt tccggcacag tgtccggcgg ctgcggcatc
     4261 tcttcgggtt ctgtttccgg ctgctgcatc tccggttcgg cggcggatgg ctgcggaact
     4321 gccgcaggcg ctggcgtggg agatgatgcc gtttccggtg cagatgcctg cacagtgtga
     4381 tagagtcgca tcaattctgc ctggtattct tccggggtct tttccatagg gatagcctcc
     4441 tgttgctttt ttgacagcat atgccgccgc agccggggat atgcaaaaaa gcctgtcctg
     4501 ttcgggcaga ctttttcacg ttcaaccatt gcctgttgaa aaggctatct ttgctttgcg
     4561 gttgccggct ctgtcagcgc ctcctgccgg aacaggaagg tcatgcacca cagtgcggcg
     4621 atcgccacaa gaatataaat ggcacggctg atgaaagacg ccgcaccgcc gaaaagccat
     4681 gccaccaggt cgaattcgaa cacgccgacc agaccccagt tgacaccgcc gatgataagc
     4741 agaatcagtg cgagtttatc ccacattaca aaatccacct ctttccggca gccccctgta
     4801 aaaatacttg tccgcttttc acggctgcac agggttagtt ttcccacggg atcggggaat
     4861 tatacgccgg ctgggggaag aaatcgcaaa tttcgtgaaa attcgacaaa aagctgcgtt
     4921 gatacgacgt attttttcat aaggaacatg gatttcacat cctgcttttt gtgcagttct
     4981 acaaaaaacg tagaaaaaac ctgctttatt ttattttcac ttgacggcgt gcgtgcaatc
     5041 gtgtataata aaaacagtga taataaaact ccgtgcaaga ggagtggctg tggattgata
     5101 aaatccttgc tttttttatt ttttcataaa cgattatcac tcgttttgtt gtctcctttc
     5161 ttttgttcta tattttgttt ttgcccgttc ctgtttggga aacgggtctt tttttaccct
     5221 ttttgagaaa acgcaaaaaa caaaatcgcc tgtccatcca gtccgatggc gcaggcgatt
     5281 tttttatatg tttattgttc gggaatcatt ttcgacagcg gattgtccgc agcttgtccg
     5341 ccgggtacag cgcttgtccg gaatcgggga aaaccccgtt tttgaaaaac gaacgaaaaa
     5401 taatttacgt tagaaaagca atgcgctttg cggcttactt gctcttgatt gcagcctgtg
     5461 cagcagccag tcttgcgatc ggcacacgga acggagagca ggaaacatag tccagaccga
     5521 tctcgtggca gaactcaaca gatgtcgggt cgccgccgtg ctcac
//
LOCUS       DAWENO010000135         5552 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5640_length_5552_cov_9.483718, whole genome shotgun sequence.
ACCESSION   DAWENO010000135 DAWENO010000000
VERSION     DAWENO010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_5552_cov_9.483718"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1308 a         1426 c         1326 g         1492 t
ORIGIN      
        1 ttgtatcacg gcgcaggctc tttggagaca ctgaaaacac tgaccggtaa aaaagccatt
       61 gttgttgtgg gcggcggttc catgaaacgg tttggttttc tggacaaggc ggtcgcttac
      121 ttaaaggaag ccggcatgga agtacagctg tttgaaggcg ttgagccgga tccttctgta
      181 gaaaccgtta tgaagggtgc agaggcaatg cgtgcctttg agccggattg gatcgttgcc
      241 atgggcggcg gttctccgat tgatgctgcg aaagcaatgt gggtattcta tgagtatccg
      301 gacacgactt tcgaagagat cattacgccg tttagtttcc ccacgctgcg gacaaaggca
      361 aagttctgtg cgattccgtc tacctctggt actgcaacag aagttaccgc tttctctgtg
      421 atcacagatt atcacaaggg catcaagtat ccgcttgcgg actttaacat tacaccagat
      481 gttgcaatcg tggatccgga actggctgag acaatgccga agaagttggt tgcacacact
      541 ggtatggatg ccatgacaca cgcaattgag gcatatgtgt ctacgctgca ctgcaactat
      601 accgaccccc tggcactcca tgcaatcaag atgattcaca ataacttgaa aaagtcttac
      661 gatggtgata tggatgcccg tgcagcaatg cacgatgcac agtgcctggc tggtatggcg
      721 ttctcaaatg cactgttggg tattgtacac tctatggcgc ataaaacagg tgcggcttat
      781 accggcgatc atattattca cggctgtgcc aatgctatgt atttgccgaa ggtcatcaag
      841 tacaattcga agaatgcaga ggcggcagaa cggtatgcag agattgcatc ctttatcgga
      901 ctgtccggcg caacaactga gggaaaagtg gatgcgctga ttgcagaact gcgttccatg
      961 aacgatcagc tggatattcc ccagggcatc aagaattatg gcaagagcgg cgtaaaggcg
     1021 gatgtgtctg ttattgatga aaaggaattc ctggagaaac tgccggaagt ggcaaagaat
     1081 gcgattgcag atgcctgcac cgggtcgaat cctcgtcagc cctctcagga ggaaatggaa
     1141 aagctactga aagcttgcta ttacgataca gaaattgact tctgattttg aagaatagaa
     1201 gtacatagat caaacggcac acgtcttgtt tttacaaaat gtgtgccgtt ttgtttgata
     1261 tagtaaaaag cgcctgtact cgaaagtaca ggcgcttttg tatattgcga aggtgaaacc
     1321 ttgtgcttat gcgaacaggc tcttgcctgt catttcttcc ggctgcggca gttccattgc
     1381 ctccagcatc gtcggagcga tatctgccag aacaccgcct tcacggagct tgcagtcctt
     1441 gccgactacg ataaacggaa ccggattggt ggtgtgtgca gtgaacggtg tgccgtcctc
     1501 ttccagcatc ttatccgcat tgccgtggtc agccgtgata aacgcaatac cgcccttctt
     1561 catgatagca tcaaccatgc ggcctacgca ggtatcaact gcctctacgg cagcaacagc
     1621 ggcatcaaat acgccggtgt gaccaaccat gtcgcagttt gcatagttca gaatgataac
     1681 gtcatacttg tcgctgtcga ttgcctcaac aaccttatca gtgacttcgt atgcgctcat
     1741 ttccggcttc agatcgaatg tgggaacgtc cgggcttttt acgaggatac gatcttcgcc
     1801 gtcgaaagtc tgctctctgc cgccattgaa gaagaatgta acgtgtgcgt acttctgtgt
     1861 ttcggcgata cggagctgtg tctttccgca cttgctcaga tattcaccca gagtcatgtg
     1921 cagatcttcc ggcggatatg caacggatac attcggcatt gctgcatcat actgtgccat
     1981 gcagacgaac tgtaccggga agtagccgtt ctttctctca aatccgctga aatccgggtc
     2041 aacaaagcag cgggtcagct gtcttgcacg gtccggacgg aagttgaaga atacgatgct
     2101 gtcgtcttcc ttaacggtgc cgttcttatc cagaacagtc ggcagcatga attcgtctgt
     2161 tacgtcgttt gcatagctgt cctcgattgc ctttaccgga tcagcgtctg tctgtacgcc
     2221 ctcgcccaga acgattgcat cgtatgcctt ttctacacga tcccatgcgt tgtcacgatc
     2281 cattgcatag taacgtccgg aaatggttgc aaccttgcct acaccgatct cgttcagctt
     2341 tgcgacagca tccttcataa atccagcagc agatgccggc ggttcgtcac ggccgtccag
     2401 gaatgcgtga acatacacct gagacagacc cttgcgtttt gccatttcca gcagtccgta
     2461 cagatgagaa atatggctgt gaacgccgcc cggagagagc agacccatca ggtgcagtgc
     2521 agttcccttt tccttgcagg catctatagc agcagaaatt gctggatttt caaagaactt
     2581 tccgtcttca atgtcctttg tgatctttac cagcatctga taaacaatgc ggcctgcgcc
     2641 gatgttggtg tgtcctactt cagagttgcc catctgaccg tccggcagac ctacgtccag
     2701 accgcttgcg ccgatcagtg tattcggtcc ctgcatgaat ttgtccaggt tcggtttctt
     2761 tgcagcatgg attgcattgc cgtaatcgct gggattgtgg ccaaatccat ccatgataat
     2821 caatgcaaca ggttttttgc tcatgttgta tttctccttt tcctcaaaaa agcagcgccg
     2881 tgcattcgcc ttacggcttt gtacggcgct gcccgattgc aaatgaaatt gtgtacttaa
     2941 aagcttactt agaagcctca gtcagcagta cgccgaactt ttcagcaacc agagatgcac
     3001 cgccgatcag accgccgtcg atgttttcct ttgccagcag ttctgcgcag ttcttttcgt
     3061 tcatagaacc gccgtactgg atggtcatgc cgtctgcaat tgccttgtca tacagagatg
     3121 caacagttgc acggataaat gcgcaaactt cttcagcctg ttctgcggta gcagttttgc
     3181 cagtaccgat agcccatacc ggctcgtaag cgattacgat gttctttacc tggtcagcag
     3241 tcagaccgtg cagagcaacc ttaacctgct ttgcaacaac ttcttctgtg atgcccagtt
     3301 cccgttcagc cagtgtttca ccaacgcaca ggataacagt cataccagcc tcgattgcag
     3361 ctgtggtacg cttgtttaca gtttcatcgg tttcgccgaa gtactgtctg cgctcggagt
     3421 gaccaacgat aacatacttt acgcccatct cagtcagcat atcagcagaa atctcgccgg
     3481 taaatgcgcc gctcttttcg aagtgaacgt tttctgcgcc aacttcaatg ttagtgcctt
     3541 cggtcagaga cagagcagtt tccaggtttg tgaacggtac gcagatgatt acgccgcagg
     3601 tcttgtctgc tgcgatcggc ttcagagcgt taatcagctc agcagcctct ttgcggttct
     3661 tgttcatctt ccagttgccg gcaataattg cctttctcag tgccttgttc atgacaaatt
     3721 cctcttttct cttattcgga tgcctcagca tccgtaatat aatctccctc ttcgtgggat
     3781 tctttattgt tgcttccgtt atgactgccg atcacgatgc ctttggagaa aggcatacag
     3841 agcattccga ttgccagacc acagaatgca cccagtgcaa atgcggctgc cggattcaga
     3901 aatgcacttt ttacagtatc ctgcaattca cactttttca tgagaatcat cctttctcaa
     3961 gacaacacaa acggaggatg gctgccgaaa cagtattccc ctgtctgttc agaaatcagt
     4021 tcttatccgc aattgcagca acacccggca gaaccttgcc ttccaggtat tccagagaag
     4081 cgccgccgcc ggtagagatg tggctcatct tatcagcaaa gcccagctgc ataacagctg
     4141 ctgcagagtc gccgccgccg atgatggtgg ttgcatcggt atctgccaga gcctgtgcta
     4201 cagcaacagt acccttgctc agaaccgggt tctcgaatac gcccatcggt ccgttccata
     4261 caacggtctt tgcggtctta acagcatctg caaacagagc ctgtgtcttt tcgccgatat
     4321 ccagacccat tctgtctgcc gggatcttgt tggaatcata ggtgacaact tcgatctcag
     4381 cgtcgatcgg gttcgggaac tcagcagcag ttacagtatc aaccggcagc agcagagtct
     4441 taccgtttgc ctttgccttt tccagcattt ccttgcagta gtcgatcttg gtgtcgtcta
     4501 ccagagaagt accaacttca tagccctgtg ccttcaggaa ggtgtaagcc atgccgccgc
     4561 cgataatcag tgtatcgcac ttttccagca ggttggagat aacattcagc ttatctgcaa
     4621 cctttgcgcc gcccaggatt gcaacaaacg gacgaaccgg gttctcaaca gcattgccca
     4681 ggaacttgat ctccttctgc atcaaatagc caacagcagt ttcctttacg aattcggtta
     4741 cgccgacagt agagcagtgt gctctgtgag cgctgccgaa agcatccatt acgaatgcgt
     4801 ctgcgataga tgccagatcc ttgctgaagg attcgatgtt cttggtctct tccttgcgga
     4861 aacgagtgtt ctccagaacg atgatctcgc cgttctgcat cttgtctaca gcagccttta
     4921 cggtatcatc tacaacaaca tcagacggaa cgaaggtgac ctttgcggaa accagttcgc
     4981 ccagacgagc agcagcagcc ttcagagaga acttttcttc cggaccattc ttcggcttgc
     5041 ccaggtgaga gcaaagaata acctttgcgc cttcagaagt cagcttgttg atggtcggca
     5101 gtgcagcctg gatacggttg tcgtttgtga taacgccgtc cttcatgggt acgttgaagt
     5161 ccacacgaac cagaacacgc ttgccggtta ctttcagatc gtctacagat actttgttta
     5221 actctgccat ttgtttgaca tcctttctga gataggctgc atcgctaaaa aaggttacga
     5281 tgccacctta aaattaacag attttctgaa atgccggttg gtgcatttca tacagtttta
     5341 ttatacacca tttcacaaaa ttttgcaagc ttttccgcaa gattttccga aaagtccaaa
     5401 attgcctgtg ggaaaatata ttttttttag aaatccgcca gttttgtcgc tttaccggtt
     5461 ctccaggtat ggtttcagat attgcccggt gtaagactgc ggacagtctg ccacctgttc
     5521 cggtgtgccg ctgacaacga cagtgccgcc tc
//
LOCUS       DAWENO010000136         5536 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5665_length_5536_cov_12.370735, whole genome shotgun sequence.
ACCESSION   DAWENO010000136 DAWENO010000000
VERSION     DAWENO010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5665_length_5536_cov_12.370735"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1420 a         1327 c         1433 g         1356 t
ORIGIN      
        1 acaagccgat ggtgccgcag gtcatgaaaa aggtcaccat cgaggactaa tcgaacgaat
       61 gaaaaaagat tgccgcagca gacactgttt tcagttttct gctgcggcaa tttggataga
      121 ccggaaatct gccggacggt atgcgccgtt tccggctttg gaaggacgat atcatatcat
      181 gggatacaaa acaaaacagc gggaatatat cctggctttt ttgcagaaca gcgagggcag
      241 gcatattact gtagcagagc tggtgcggca cttgcaggac aatgccacgc cggtgggaac
      301 tgccacagtt taccggacgc tggaacagct ggtggaagag ggcatggtgc ggaaatatgt
      361 gctggacggc aagagcagcg cctgctatca gttcctctcc gatgagccgc atctgtgcca
      421 ggagcatttt cacttgaagt gtacctgctg cggcaggctg ttccatgtca gctgtgacta
      481 tctgaatcag ctgggcgagc atctgctgga acatcacggc tttgtgatcg atcatacgaa
      541 aacggttttg tacggtctgt gcgccgactg cgctgcaaag gaacaagaga aaaagatgta
      601 agcgcctgcc ggcgtatcta tagcgtgtat cgaaaatatc tcgaaaaaca agcaatcaaa
      661 gtaatctccc tgtcagctta tgctgacatc ccagcacaag gcacacccta cgggcgcctt
      721 ttaagtggga cataaaaaag taggcgaaaa gatacgacag caggctggtt tcgcagccgg
      781 tgggttatcc cagcggcgaa gtgggctggt tgggatgata cggccgctcg tagatccgca
      841 ccggtgcgtt gtcaaatgcc acattgtcgc atttttcctg gatcacccgg tggggcgtat
      901 agatcttcat ttcggcttct gcactttgtc tggctttttc ctgttcggga ttcatggcac
      961 tacctctcat aaataagctt tgaggcgttc catatacgcc tcttcgtgac ggacaaacgc
     1021 tttgccctgg cgtttcagct gatcggaaat gccgggattg ccgtgcagca cctgggtcag
     1081 ctggatgacg ccggtattgg tgcctttcag catcagcttt gcgatctcat tggaagatgc
     1141 gccgcccagg cagtgcatct gaatgctggc gtttgcccag aatttcgcca tatcgttctg
     1201 ttccgccggc tgcacatggc ttttctgcat ctggctgcgc agggtgtctt tctgggtctg
     1261 atagaactcc agctgtttgc aaagctcctg ccgcagtgcc gggtggtctg ccttagggag
     1321 aatggtgctc gtggcatccg ctcccatcac ggcatttttg taaaattcgt ccagtacgtg
     1381 atgttcctgt ggagacatga ctttgccctc ctttttgggt cggcagaggt ttcaaacgtc
     1441 tgccattgct gttatttttt ccggagaagc atccgatata ccgaggaatc cgtttccaca
     1501 ggaggtgaaa tatggaattc aaaaattttg ccaaaggact cggcacatat ttctttgcgg
     1561 aagtgctgtg cctgtttctg gcggtcacgc tgtctgcggt gggcggcggt ctgttccggc
     1621 tcatcagctg tatctgcacc attgcagtgc tggtctgtgt ctgcgtcaac tatgcgctga
     1681 atcgtgccaa agaggatcag caccggaaac tgcccaacac catcggacgg cagctgtttt
     1741 acagtgttgc ggcggcgctg ccgtttctcg tgctggggct gacgctgtgc ctggcgaaag
     1801 caggcgtatt gccggaacac tggtatcggt ggtacaagct gctggatgcg ccgtttttgc
     1861 agctctgcaa cctttgcagc atggatgtga ctgtgtcgtc tctctcctgg ggaaaaacca
     1921 tttttctggc gctctggaat ctgctgcctg tggcggcggt gtgggtaacc tatgcccttg
     1981 cccgaagcgg gttcctgccg gaggaactgc agtataaaaa gcggcgttcg tgagcgtatg
     2041 tgtattataa tgaaaaaagt gactggattc agtcactttt tttcggggtt gattttctga
     2101 gataatctgc cagcggcaac gtttcaatgg gcgcatatgc cgctggttct gcatccaggg
     2161 aaagaaacgc atcccactgc tccggtatct ggagcagctg ttccttttgg gtgcgggtca
     2221 gctgcttgaa agcgcattca aaccgggcgg cggcagtatg gtcgacagtt tcccacaccc
     2281 ggagcagcgc ctccggtttg tggctgtggg tgtattttgc gccgccggca agctgaccgc
     2341 agtgctgacg catccgccgc cggagatccg gggtaatgcc ggtataaagt gtatcgccct
     2401 tgcagcggag tacataaata tagtatgcca taggaaagcg ctccttttgc gaaaaatgta
     2461 ccctgttatt ataacacacg gtagaaaacg ctgtcaatcc gaagataagc cgtaccgcag
     2521 gcgcagatat tgtgaaaaat gctgtttttt tgcgaaattg cagaccgttt tttgaaaaaa
     2581 tatccggttt acagagggcg aaaactgtgg tataatgaaa tgtagaagtt tttctgccat
     2641 aaaacaacga tttttaatgc tattaggagt aactacagat gagtaaaaag agtacagaac
     2701 agaaaccgga agttgcaact gttgcactgg tgcagagcgc acaggaagag gaagatacca
     2761 ttaccattcc ggtgatggat attttccggc agatgaaaaa atattttctg ccctgggtgc
     2821 taatcgcagt gatcgcagcc ggcgccgtat tcggcggagc aatgctgctc tccaacagca
     2881 aggcaacacc gctgaccgct atggtcggct ttacctatga cggaatcgaa aagggtctgg
     2941 acccgaacgg caacgagttt gatgccaaca ccttgaaatc cccgtccatc attgaaagca
     3001 cactgacaga tctgggcatg gacacaaaaa ataccgatgc catccgcagc gccatcacca
     3061 tttccggcgt tgtcccggac gacaccattg acgagctgac cgcttacaag agcatcttcg
     3121 aatccaccaa ttccgtggat gccgcacaga agctgatgga tgtgtcctat tatccgacac
     3181 gttttgagat ccagtttgct tacggcaatt ctaaaatgaa ccgctctcag gcggcagatt
     3241 tcttgaacac catgctgagc aactacaaga cctacttcat gcggacttac ggctacaacg
     3301 atgccctggg cgatgcgctg actgctgtgg attataccac ctatgactat ccgcaggcac
     3361 tggatgtgtt ctcctcgacg ttgaactctc tccagaacta cgtatccacc ctttcgcaga
     3421 acgacaacac acggttccgc tccagcgata ccggttatac tttcgccgat ctgaacgagt
     3481 ctatcaacac cctgcgcagt gtggactacg caacgctttc ctcttatgtc ctcggcaaaa
     3541 acgtgacaaa ggacaaggca tccctggcga tctactatca gtaccgcatc gacaatctga
     3601 accgcaatct gagcagcgca aatgaaaagc tggcaacgat caccgactcc atcaagaact
     3661 accagaagga ttccatggtg gttatggctg gcgcagatgc aaccagcagt tctgcactga
     3721 cacagccgtc tgaggcatat gatgatttga tctcccagaa gacagatgca cagagcagcg
     3781 tatcttctta tcagcagcag atcgcagatt atcagtcccg tctggacaag ctgaacagct
     3841 ctgctgtggc aagcaaaaag gaccaggaaa aggtcgagac tgacatgaag accgtatgcg
     3901 acaagctgaa caagctgatt gacgatgtta atgcaactgc agacgattac ttcgagactg
     3961 catcttatgc aaattcttac agcgttctgg ttccggcaag cggcagcgtt tccaacgcag
     4021 tgtccaatgc agtatccaac atgatgcgtc cgctgctgat cgctgaggca ctcctgtttg
     4081 tatgctatct ggtatttgcc attgttcgtg catttatcgt ttcttaccgc agaaatgcca
     4141 ttgcaactgc tgctgtagag gcagaagaac gcaaggaggc agcagaggct gcggaaaaga
     4201 cagaagagac ggaagaataa aacgtttctt gtgtcttaaa agcaagtgaa agcagtttat
     4261 cattaaattg ctcctcaatt gttagacaat gtgatgtgat tgaaatagat tcatgatatg
     4321 tctgacaatt ggagagcttt ttttgaacct gtataccaat catggggcat cggtttcgaa
     4381 aaaagtctta cagaaaaaca ggatgcagaa gattctgtgt cctgttttct tagcgaaaat
     4441 atttgtcgaa ttttgtcgaa aaaaggggaa taacatgaaa atgggcatca aaagcaaccg
     4501 tttatgcagt gccggagcag cacttctgat cttgctgaca tttctgctga gcgcatttgc
     4561 cggcggcagc gtgtatgcag agacaacttc caagaaccag ccaatccaga tcagcagcgc
     4621 agaagaactg ctgcaattcg cagcagatgc cgccaaggac agcagcatca acgcagaact
     4681 gaccgctgat attgatatga cgggaaagga cgcctggact tcgatctctg aatatggcgg
     4741 gactttcaac ggaaattatc ataagatcac tggacttcga tgcagtggcg gcagaaacac
     4801 cagtttattt cagaaactaa cgggaaccgt agaaaaccta tggctggaag gaatcgatgt
     4861 ttctggttca aagcaggttg ccggaatcgc atccaattgc ttaggaaaaa tacaaaatgt
     4921 ttttctggag ggaacagtca ccgccagcgg agataatgcc ggcggtttgg tctgtgattt
     4981 acgagatcct ggttttattg aaaattcctg tttcatcgga actgtaacag cagctggcgg
     5041 aacgattggc ggtgtcgttg gaacctgcta taccaatatt aaaagctgtt atttcctagg
     5101 aacaattcaa tttacatcac ctaacgcaac agttggatta ctgacaggat atctgcactc
     5161 tccaacaaca gactatttta catattgctt ttgtgctaaa aacattttaa ttcctgccat
     5221 tgggaatgtt actgttcccg aacacgtttc aatttgtgaa gaatccgcat tcgccgacgg
     5281 aacagttgcc gcagcactga atggcaacga gggcagcgat gcggtctggt gggcgcagga
     5341 cggaaagacg atgctgaaag cgttcggcgg aaaatcggca gaaacaacga cttccggcac
     5401 aacaatgacg acaaccgcca caactgccgg taaaacaacg gtttcttccg gcgaaacggt
     5461 tacttcggat agtgcggctg ttagtgaaac aacaaagctt accactactt tcacaagttc
     5521 aactaccagt acaaca
//
LOCUS       DAWENO010000137         5533 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5668_length_5533_cov_10.528295, whole genome shotgun sequence.
ACCESSION   DAWENO010000137 DAWENO010000000
VERSION     DAWENO010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_5533_cov_10.528295"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3078..3087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1389 a         1310 c         1605 g         1219 t
ORIGIN      
        1 cgcactgcca gctcagcaca aggcacgccc ttcgggtgcc ctttcagggg agccttactt
       61 gtcaaacttc ataattttaa tcagcatatt tttgaaaatt gatttcgctg ccgttctgcg
      121 gaaagcaagc cgccttgcat ctcctgccgt ttcatggtat aatacaagta acaacaaaca
      181 gcaaaggaga acgctctatg gcaaaaccga aaaccattta ccgctgtacg gaatgcggct
      241 atgaaagcgc caagtggaac ggaaagtgtc cgtcctgcgg cgcatggaac accctggagg
      301 aggatgttcc gctgccggcg caggcggtgg gaaacagccg ccggaaaccg gtggatcttt
      361 cggacaagat acaggaactg gacgaggtgg acaccacggc ggatgtgcgc tatcacaccg
      421 gcgtaggcga actggatcgt gtgctgggcg gcggtctggt gcgtggctct atcgttctcc
      481 tgggcggcga gccgggcatc ggaaaaagca cgctgctgct gcaaatttgt cagtatttcg
      541 gggaatctca cagcgtgctg tatgtctccg gcgaggaatc tgccaagcag atcaagcttc
      601 gtgcgcagcg tctgggcgtg acaaccagga gcctgtacct gctgacggtc acggatgccc
      661 agagcatctg cgacaccatc tccgccagcc gtccggatat tgtcatcatc gactccatcc
      721 agaccatgcg tatggaagag atctcttcca gccccggcag tctgacccag gtgcgggagt
      781 gtaccaatct gtttatgcac actgcaaaac agctggatat ccccatctgg atcgtgggac
      841 acgtgaacaa ggacggtgcg attgccgggc caaaggtcat ggagcacatt gtggataccg
      901 tgctgtattt tgaaggcgaa cgcaaccttt cctatcggct gctccgtgcg gtgaaaaacc
      961 ggtacggctc gaccaatgag atcggcgtat tcgagatgca ggacagcggt ctgcacgaag
     1021 tccccaaccc gtctgccatg ctgctggacg gcagacccca gggcgtttcg ggaacgtgtg
     1081 tcacctgcat gatggaaggt tcccgtccga tcctggcaga gatgcaggtc ctggcgacaa
     1141 aaagcggatt ctctgcgcca cgccgccaga cccagggctt tgactacagc cgcatggtga
     1201 ttctgattgc cgtgctggaa aagcatctgg ggctgttttt cgggacgctg gacgtgtatc
     1261 tgaacgtagt cggcggcttt cagctggacg aaccggcagg cgatctggcg gcggcgctgt
     1321 gtctgtattc ctctctggtg gacaagccca tctcggaaaa aacggtagcg ttcggcgaag
     1381 tgggactggg cggagaagtc cggggcattt cgcacatccg gcggcgtgtc cgggaggcag
     1441 agcgcatggg ctttacacgc tgtatcgtac cgcagcagtg cctgcacgaa ctgaaaggcg
     1501 gctcgtatca gatccggatc gccggcgtgc ggaatctgcg gcaggcgttt tccgtgctgg
     1561 aaaaggatgc gagagaggct gcggaaaaat aatatgggca gagcgtgtct gtataaaatg
     1621 tctgtgcgga aaaatttctc cgcaggcgct tatggggaca cactccggtt gaggagaaag
     1681 caaagatgtt gaaaatgtat ctgcaaaaag gaattgcagc agcggctgcc gctgtgatgt
     1741 tggtttgttc cgtcagtctg gaagcattcg ccgactcgtc tgtgccagaa agcacggcgc
     1801 ataaggtgtt tctggcgatt tcagctggca caaatatgag atgggttccc gatgacgaca
     1861 gttccgctgc taaaaaaaac agcacaaccg gaaaagacgc cgttatcaca gaagacggca
     1921 gttattcagt cagcgctgca atcaacagca gcacagattt tataaattgt ctgatacttt
     1981 ccacggatat aaacgcatac gattacattc cggatggagc aagtatcccc tattacggat
     2041 tgcaggatac ggaatgtacg ctccatctca cagtagattc cattgcggta cagcatccgg
     2101 acggcaccgc tacgcctttg acatataccg gaccgtctga cggcgcactt cgtgccgcag
     2161 atgacggcaa aagcattcag ctgaatattt tgaatgtctg gagcagaccg aacatccatg
     2221 atctgcgttc cgaagaatct tctgacgagt atgtcaaggt gacggatggc ttgcaaccaa
     2281 acgatcaagt ggttgtcagt ttcaccatca ccggactaaa atccggtaat acagcagatg
     2341 aaaccccgga acacgaactc gaactcgccg atctggacgg cagcggtacg ttggattcca
     2401 cagatgtgtt ttaccttatg ctgggcattg cgcagagtgc cgtgggaatc tctcccgact
     2461 ggacggcggc acaggaaaag gcggcggaca tcgacggcag cggcaaggtg gattctacgg
     2521 atgtgttcta tcttatgctg tatattgccc ggaaaggcgc aggaatcccc accacgtggg
     2581 aagatattat cggataaaag gagaatcata tgaaaaactg gaaaaaacca attgcggcag
     2641 ttcttgccgg aatctgttgt gttgtaggta tgccgcaggt acagctggtt ctgccggcat
     2701 tgcaggctgc tgcggcagag actgcggaca ccggaacagt cgggacgttg acctatactt
     2761 tagaatctga ccgggctgtg attacacagt gtgatgaaaa tgcggaagaa gtcgtgattc
     2821 cctcagagat cgagggaaaa ccagtgctgg aaattggtga aggtgcgttt caattttgcg
     2881 aaaagctgac cagcgtggaa atcccggata ccgtacagac aatcgggaaa aatgcgttct
     2941 ggagctgtca caaactgcaa gatgtgacgc tgccgaaaaa tctggtttcc gttggagaca
     3001 actgctttcg ttcctgcggc ttgctggaaa agctggatat cccagaaaca ctgcaaaaaa
     3061 ttggattgga tgcgttcnnn nnnnnnnatg gctgaaaaac cagcaggcag tgaatccgct
     3121 ggtacaggtg aaccatattc tgatcgacgg ggcaacatat tccggcacaa cggcagaaat
     3181 accgggcggc attacggaaa tcggtaactt tgccttttat cagtgcgaaa atttgcagaa
     3241 ggtcatacta ccggaaagtg taacaaaaat tgattggggc gctttctggc agtgttccag
     3301 tctggaaacg ctgacaactc aagggaattt aactgagatc ggagagtccg ccttttcaga
     3361 ctgcacaaag ttatctgctg tgtatacggc tatggcagag gacgagtgga acaatattca
     3421 gatcgaatcg gggaacgagt cgctggcaca ggctgtggta cattataata gtacatttga
     3481 gacattgctg ttaggcgaca tggacggcag cggcacactg gattccacag atgtgtttta
     3541 cctcatgctg ggcattgcac aaagtgctgt gggaatctct cccgactgga cggcagcaca
     3601 ggaaaaggcg gcggacatcg acggcagcgg cagggcggat tctacggatg tgttctatct
     3661 catgctgtat attgcgcaga acggcgcagg gattcctact gtatgggaag atattatcgg
     3721 ataaaaggag catcctatga aaaactggaa aagaccaatt gcggcagttc ttgccggaat
     3781 ctgttgtgct gtaggtatgc cgcaggtaca gctggttctg ccagcaatgc aggctgcggc
     3841 ggcagagact gcggacaccg gtacggtcgg ggcgctgacc tatactttag aatctgaccg
     3901 ggctgtgatt acgaagtgtg acgaaaatgc ggaagaagtc gtgattccgt cggagatcga
     3961 gggaaaaccg gtgacggaaa tcggagagag tgcctttcaa gcctgtgaaa gtctgacccg
     4021 tgtgacaatt cccgacaccg tacagactct gggaaaatat gtgttttggg gctgcaccaa
     4081 gttgcaggat gtgacgctgc caaaaacgct gagcgccatt tctgacggct gttttcgctt
     4141 ttgcagtgcg ctggcagagc tggacattcc ggaaacggta gagtacatcg gcatagatgc
     4201 gctgggcagt acagtgtggc tgaaacagcg ccaggcggaa aatccgctgg tgcaggtgaa
     4261 tcatattctg gtgaatgcca ctgcgtgtac ggacagcaga gtcgtgattc ccaacggcgt
     4321 gaagcagatc ggaccgtatg cgttcaacag tacagcggta acggaagtag ttattcccga
     4381 aagtgtggaa cgcatcggct ggggcgcatt ctggaactgt cagagtctga aaaagctcaa
     4441 tcttccggcg aatctgaccc aaatcgaaga aagcgctttc tgcaactgcg ccgcattgca
     4501 ggaattggag attccggctg gcgtgacaag gatctttgac aatacatttt ccggctgcac
     4561 cagtctgaaa cagatcacca tactgggaaa tctcacacag atcggcacgg ttgcctttta
     4621 caactgcacc agtctgacgg atgtatatac ggcgatgaca gaaaccgcat ggaaagcggt
     4681 ctatattgct caaaaaaacg aaccgctgga acaggcagcc atgcactatg acatattcgc
     4741 agggctgctg ttaggtgaca tggacggcag cggcacgctg gattccacag acgtgtttta
     4801 ccttatgctg ggcatcgcaa agagtgccgt gggaatctct cccgactgga cggcagcaca
     4861 ggaaaaggcg gcagacatcg acggcagcgg cagggcggat tctacggatg tgttctatct
     4921 catgctgtat attgcgcaga acggcgcagg gattcctacc acgtgggaag atatcgtggg
     4981 ataaccaaga aattttcctt gcatttttct ctgtcatgtg gtacaatagg ataggacttt
     5041 ccgggcgttt ccgggctgtc cgatatccgg aaaaacagcg gtatttgccg tttttgccgg
     5101 aaatatatga ttttgcgagg aacatagaca atggtagtca ttgaaatgga aaacggaaag
     5161 aagatcaaga tcgaactgta tccggagatt gcaccgatct cctgcgagaa cttcgaaaag
     5221 ctggtaaagc agggctttta tgacggactg atcttccatc gtgtcattcc gggctttatg
     5281 atccagggcg gatgcccgga tggtaccggt atgggcggtc ccggctggaa tatcaagggt
     5341 gaatttgctg caaacggcgt aaagaacgat ctgaagcaca cccgtggcgt gctgtccatg
     5401 gctcgtgcca tgaacccgaa ctccgcaggt tctcagttct ttattatgca cgaggatgcg
     5461 ccccatctgg atggtcagta tgctgcattc ggcaaggtcg ttgagggcat ggatgtggta
     5521 gacgagatcg cat
//
LOCUS       DAWENO010000138         5497 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5718_length_5497_cov_9.470783, whole genome shotgun sequence.
ACCESSION   DAWENO010000138 DAWENO010000000
VERSION     DAWENO010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_5497_cov_9.470783"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3892..3901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1250 a         1539 c         1403 g         1295 t
ORIGIN      
        1 tagaggaggc gttctcaaat tgtctcctct tccagaggag atgcccgaca gggcagagga
       61 gtcgattctg tttcacgtga aacaatccaa aacagccccc aaaaaacaac ccatggagga
      121 taaaacaatg gcagttacat ttttgaccaa agacaatttc aaaagcgaag tcatgcaggc
      181 ggacaagccc gtcctcatcg acttctacgc agactggtgc ggtccgtgcc agatggtctc
      241 cccgatcgtg gacgagatct ccggcgagcg cagcgacgta aaggtctgca aagtcaacgt
      301 ggatgaacag gtggaactgg cacaggcgtt cggcgtgtcc agtatcccca ccctggcagt
      361 gatctccggc ggcaagctgg tgaaccacgc cgtcggcgca cagcccaagt cagcgatcct
      421 ggatatgctg gatgccgcaa ccaaataatg gatgcaccca tcaaaaaagc accgatctgc
      481 gaggttttct cacaggtcgg tgctttttgt gttatgggaa aatgtctcac gaattacgag
      541 atcattccaa gtttcacgta gaacagcgcc gcaaaaactg caaagccgcc gcagagcgca
      601 tagccgatgc ggtacagcca gcggcagacc ggtttttcca tctgccgcag ccgctgaaat
      661 gccagcccca ggaacactgc tccgatcaca caggtgggcg taatgtagcg gtagttcatg
      721 gaacaggtaa agggtgcgct tgccgccagc tggtaaaagc taaacagcag caccccgtaa
      781 aatccaacca gaaacagccc cggcagtctg cccagcgtct tgtgtttgcg ccagaacatc
      841 cacaccatag cggcgaatgc ggcggcagcc agaagaacct ccgtccagaa cagcaccaca
      901 cccagactga acgccagggg atgtccgtcc acggtgtatt ggttgatgta ctccccgaat
      961 acggcatttt tcagcagcgt gataagggga ttgtattcgt tgtagccgga tgcgtcatag
     1021 tggagccact gctcgaaaac ctttgccacc tggtgcggcg aaaagtccag cgctctcgac
     1081 aaaaaggact ggtcgcccag gtactggtcg gacttcaccg acatctcctg cacatagttc
     1141 agcggcacgc cgaaccgcac cagattccgg atgggatacc agagtcccag cggtgcgcag
     1201 atgcccagaa aagcaccgta ttgcccgaaa atgcgccagt ttttcgcctt gcaccgccgc
     1261 accagaacca ccaggaacac cgccccgatg ggaaatgccg ccagcgccac agacaacttc
     1321 gtcatcattc ccagccccat gtacagggcg attttcagaa tgccttccca ggtctgtttc
     1381 tcgtaccatt tcagcgtcca aagcactgct ccgagcagaa acgccacgga cagcacatcg
     1441 ttgttgatgc tgccggaaaa gaggataaaa ctggggtgaa atgcgatcac cgccagggga
     1501 aaatacagcg ccggaccttc cagcctgaag tgccgcagga tccggtaggc agtaatgatg
     1561 accgccgtgg cgtagaacag cgtcagcgtt tgcagggatt cctgcgctac ctcgctgccg
     1621 atgccgaaca cattttcgga aagccacagc cagaccgccg aaatggcgtg gtgcagcggc
     1681 ggatggtaga actgccagtg atcccgtggg tcgaaatccg gcagacagtg atgctccagc
     1741 agatacgtga tatagccggc atgattgttc tcatctccga aacgccccac atcgtgctgc
     1801 cgccaggtaa tgggcgtgta gcacacgtag aaaagccgga ggaaaaagct gctgccaatt
     1861 aaaaacaggg agatgcgccg ccgctggttt tgtacctgtt tccgggtgag ccagagaatg
     1921 cctgcgagaa tggcacagcc gcccagcata agccacagcc ccggatgaac cgccttgcgg
     1981 tactgccctg cacccagcag caccagcact gtgaaaccga taccagccgc tgccgctggg
     2041 atgcgtcgat ggggctgata gcgcaccagc cgccacaaga ggtaaaaacc gatgatgagc
     2101 agcgccaccg tctccagcat cagcgcattt gcgggaatcc cgttttctga accgaagtac
     2161 agtggattca gcagcaggca aaacagcagg ctgcatagat agggatggcg ttccagcagc
     2221 tgaaaatagc ggttggattt cggcgcaggc agcactgctt tatccaggat gtgtttattt
     2281 ttgctcatgg tattcctttt cctggttgac gttccagaca gcgcccttgt ctgtggagtt
     2341 gtgcttgatg gcgtcagcgg cgtggatcgc cgtcagcatg gagtgatcca tattattgta
     2401 gcggtgctgt ccgtttctgc ccacgcagaa cagattgtcg aaggtgttca gataatccac
     2461 gacctggtcg aactgggcat aggtgtcaaa atacgccggg tatgccttct tgaccttttc
     2521 ccggtgcgca tccagaacag cgccttcggt gatgacgccc atcttggtca gttccttgac
     2581 ggcgaagtcc acgcattcct gatcgctcat attccagaag tcgtcccctt ctgcacagaa
     2641 atattccaga ccgatccaaa cggtttcctc cggcttttcc accatataag gagaccagtt
     2701 gttgaatatc tggattcgac ccagtttgac acccttgtcc tggacataga tccagcaatc
     2761 cggtacaata ttgcccagtg tcggagtccc agtttgattg accagattca gcttgttcac
     2821 cagcagtccc acggtgacaa agtcccggta cgggagacct gcggcgatgc gctgcacctc
     2881 ggtgttatcg cagccgtcca tgccggcgat gagatcctga atcggcatag acgaaatgaa
     2941 gatgtcgccg gtgatggtct tgctgccctc cggcgtctca cagaccacag aagtcacctt
     3001 gccgttttcg gtgtggatgg tctttacagc gtggtgatac aggatgcggc agcccttttc
     3061 ctctgccttg tgtcccacca gttcccagag ctgacccgga ccgtatttcg gataccagaa
     3121 ctgttcgatc agcgaagtct cagcgttttc gttgtttttc ttgcctgagc gcttggagat
     3181 catgtctttc agcactgccc ggatggacag ccccttaaca cgctgtgcgc cccagtcggc
     3241 agagatctcg ctggggtgtc tgccccagag cttttcggtg tatccctcga agaacatccc
     3301 gtacagcacc ttgccgaacc ggttgatgta gaagttttcc agattggtct ccggcttttt
     3361 cgagatgcac gaacccagat agctgaatcc ggctttcatg gtggtggcaa aacccatgct
     3421 ctggatggtg ttcttgttca tggtcaccgg atagtcgaag aagtgcttgt tgaagtagat
     3481 gcgggagacc cggtcacgca ccagcatgac cacgtcgtcc ttttccggat cgggaccgcc
     3541 tttttccagg ggcttttccc gtcccagctt tttgtcgtca taggacggcg ccccctgaat
     3601 gggcatcaga tctgcccacc actgcataac ggtgtcgtcc ttggagaaga accggtgacc
     3661 gccgatgtcc atgcggttgc cgtgatagcg caccgtctgg gagataccgc ccagaacgcc
     3721 gctttcctcc aggatcgtta cgtcatattc gttttcggta tccttgagca gttcatatgc
     3781 cgccgtcaga ccagccggac cgccgcctaa aatgatgatt tttttcatgt tgtagtcctc
     3841 gttgtaattt ggatttggaa attattttga aaataataaa tttgatttat tnnnnnnnnn
     3901 nttgaaaatt tccataaaca cgaaaggtgt agttttttca caataatata aaatagacac
     3961 acctttctta ttttatcata acatagaaac tgtgctttgt caatgcggcg gcgataaaaa
     4021 aactccggtc tgccattata cagaccggag ctttaacgat tgcaaaaaga aataaccgcc
     4081 ctcagcgacc aaactagaga cggttaattc tttactttat ttgacatggg agcaaccgtc
     4141 tgccggtatg ctctttctat ttttattata cttaaatcca tccggtttgt caaggggcag
     4201 accggatttt ttgtgtatct ccggcttgac agttttcggc agaatatgct ataataacaa
     4261 tcagagtgca ttggaacggg tacatagtat gtattcggcg gtctaatcct gctcccggaa
     4321 gggagtgatt gcgatgactt ggatggagtt ttttgctttg tgcaatgttg tatgcgatat
     4381 cattttgatc ttgcaaaacc gcaataaacg gaaataaccg ccccgagcta ccaacttgga
     4441 cggttatttc ttagctaacc attgggagca gaccgtttcc gatgcgctct ttctattttt
     4501 attatactta aatccatccg gtttgtcaag tagcagaccg gattttttta ttcttttcca
     4561 cacctcactg tggaaagtac tcacccgtca cgtcatccga aacgccccgt tctgttccgg
     4621 tgcatccggc cgctttagct gtgcctgaca gagatgtttc tgtcgggtgc ggagcgtctc
     4681 ccggaatgcc agcagttctt ccggattcat gcgctccact gccgccgaaa cagcatcctt
     4741 ttccgtgcag ccctctacca gaaaccgcag ccgcaccaca tcctgccgca gtgcattctg
     4801 catgcggttc agcaaaacgt cccggttttg cagctgctgt tccagttgtt tgcagcgctg
     4861 tgcgccgtcc gtctccgagc ggaactgctt ggtgacgcct gcctcacgct gcgccggcac
     4921 agccacaaag ctccactcat aggcatccgt cacatctccc agcaccacat gacaaagctt
     4981 gtctccgtac cgctgcccta tccggtggga acagggggaa ttcctgcggt cgctgccgca
     5041 gacggagcag gtctgggatg ctgccgcaca ggagatgctc acttccttct tgatgccgcc
     5101 gtcaatttcc cggatcagat cccggttggc atcggtgcgc accatgtagg cgtgtccttt
     5161 caaaaaggta tacacctcgc cggcagccgt gatgcgttcc ggatcctgta ccagttctgc
     5221 cgcatagagc cgtgccgtct ggttctctcc cttggggttg tggtcaaaaa tgcccgtttt
     5281 tccgacaaaa agcgttttca gctgttccag cgcctccaaa gaaaaacgct cgccgtcccg
     5341 gtcgatctca ttgtcgcaca gccgtacatc aaagagaaac acctcttcct gggtcagcgt
     5401 gcgtcgggta aactggttca gctgcgccag cctaacctcc atctcctctg taccgggatt
     5461 ttccgtcttg ttttctgcca ttctgttctt ctcctct
//
LOCUS       DAWENO010000139         5476 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5745_length_5476_cov_9.355285, whole genome shotgun sequence.
ACCESSION   DAWENO010000139 DAWENO010000000
VERSION     DAWENO010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_5476_cov_9.355285"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1191 a         1758 c         1328 g         1199 t
ORIGIN      
        1 cctttgtaac acgttcctgc accacagcgc tggatgcgcc gctgcacacc acgatcacac
       61 cgctgatctc cggcgcagcg atctgctgca ccagtggctg ctgactgccg ctgtcggaaa
      121 cgatcacgca tttccgctgg atcctggtgc ggttttcgcc ctgctcctgt tcagcatcct
      181 gtgcatagat ggtctccggg ctgcctgtcg ccgtcagcat cacccgacag ctgcccacgc
      241 cctcgacctg ttccagcaca tcgaccagct gctgttccat ccggcggcag tactgctccg
      301 actgtttctg agaggctgtc tctgcgctct gcacagatgc ggcatctgtc tgcttttcgg
      361 ctttgtccgg cagaaacccg gacaacagga tcagcgccag cccggcgatg cccagcagca
      421 tcaccagccg cacgccgttc tcacccttca gctttttccg cagcggttca agaatccgtt
      481 tccacatgga tattcacctc ttttcccaga caacctgtca gcagctgtcc tgccgctgcg
      541 gcatctccac aggccactgt gacttgttct atctctatgc tgttgtcctc cgcaatattc
      601 atttccacct gaacccgggc atccggaatg ccgttctgcc gcagcatcgc cgcaagagat
      661 tcctccagat ttccggcggc acagcgcagc gtctcctgtt ctgcggttcc tgccaggctg
      721 ttttccgtaa gttcggcgac gcagtcccag ttcggctgaa actctgccgc accacggctc
      781 agcggcgtga agattcccag tacaaacact gatgccaaaa gcagccgcag ctggcggtcg
      841 aactttgcgc tgggtttcag cacgtccagc agaccgctga gaatgctgat gacacaggtc
      901 accagcacaa tggcacgcat ggtctccaac gctatgctgc cccctttcca atggtcatgg
      961 cgatggcagt tgccaccacg aacataatac cgaaacagac cagcagcgca aaggcaatgg
     1021 cgagcgcctg ctgtgttcca cggagcagac gcagcagact ttttgttccg aaaagttctg
     1081 ccgctgccgc acccaccgcc accacagcac ggtacaacag cagctgcacc agcggcggca
     1141 aaaaaagcac acacagggag acgatcccga tcacgcctgt ggtgctgcgc aggatctgca
     1201 aactgctgcg cactgtagta taggcatcgg ataccgcatt tcccaccacc ggcacaaagt
     1261 tggcgatcac atacttggtg gttttggatg ccgctgtatc caaactgccg cccagccagt
     1321 tctgcatgga aagcaccccc agaaacaagg tcatcagcaa ccccagcacc caggtggtaa
     1381 acttccggga cagccgcagc agtccgccca cggacacaga cggactgacg gcatccacga
     1441 ttgccagtga aaagcccatg gtgcagaacg gcagcagcac acgggtaatc atctgcatca
     1501 cgccgtccgc cagggctacc acggcagtct ggtaggacac agcgctggtg actgtaccgc
     1561 ctgccgccag gataccggca aacaccggca caaacagggt cataaacgat gcgccctggg
     1621 acagtgcgct ctccaccgcc gcaaagctct gacacagcgg ctctgccagc actgccactg
     1681 ccgcaagcgc acagacaaac tcgtatacct gtcccacggc acggcgtttc gggtcatcct
     1741 ggatgccctg gagcagtgcc gcaaacagga tcactgtcag cagtccgccc agcatggcaa
     1801 aaggctgtga gatgcgcccg gagaggtagt ctgagaacac ctgccagatg tgtgacagag
     1861 tgaatttttc cccggtctca tccggcgcag tcaccgacag ccccagctgt tccagcaccg
     1921 actgcacatc ccagtccagc tgttctgtca ccccggctgc accgctctcc tctgccagct
     1981 gtccggcagg ttcttcctgc gccaccgcct gccacggcag cagcagcccc aggcagagcg
     2041 tccccagcag caccagcgct ttccaccgtt tcatgacaac acctccgtta ccatctgcaa
     2101 aagggtctcc aacagcggca gggacagcag caccagggaa agcctgcccc agagttccac
     2161 cgtccgtgcc agagcagact cgccgcagtc ctggcagaga tccccggcga tgccggtcag
     2221 atagcagatg cccagggctt tccagagaat cgtcagatag gcggacggca ggtttgtccg
     2281 ctcgaaaagc gcctgcatct gcgacaccac tggcgtgaga tacgccgccg ctgcgattcc
     2341 caccgccgca cacgccccga tcacgcagaa cagggcatgt tcccggcagt attgtttcaa
     2401 cagtgtggaa aagatcgccg cagtgatgca gactgcggcg gcagttacag tctcctctat
     2461 cataagccaa acaccgactt tacccgttca aacagcgtgc cgatctgatc gaccagcagc
     2521 atcagcacca ccaccagccc ggcaagtgtg gtcatcatcg cctgctcctc ccgctctgcc
     2581 cgtttcagaa ccaggttcag cactgccaca atgatccctg ttccggcaat cttaaaaatg
     2641 agatctattt ccatggtttt tcctttcagc aaagcaggat tgccgccaga aagccgccca
     2701 gaacacccag gctgcaatag agccgttgtt tccggcggca taattcgcct gcctctgcgg
     2761 catagggttc cagctgccgg cggtattggg acagcgtggc ggtctgaccg tccaggtcgc
     2821 ttgcgcccag ttcttcgccc aggggcagca gcagattctt tcccgtctcc ggaattgcct
     2881 tgtcctgacg gatgcccgac tcccaggcat ctgccagagg cattccctgt tccagcccgg
     2941 aaagcaccag caccgggaac tgaaacgccg catatgccgg ctcccggcaa agctgttcca
     3001 gcagttcctg caccgtcaga ctgcgaaacg ccatctgcac ggaaagttcc cggagcatcc
     3061 ggcagagctg ttccaggttc cggtgtgtct gccgcagacg catcgcctgc atcacgccga
     3121 tcccggcgcc gcagcagacc accagcagca gtcccagcag tttcatcatg gcaccacccg
     3181 gcgcagcgtc tgcacctttc cctgttccct gccggacaaa actgccgcat accgaaacac
     3241 gcccttgtcc agcagctgtt tcagcgccgg ccggcgttcc aggtcttcca gagatgttcc
     3301 gtgtgccgat gccaccaccg ggacgccgct gccgtgtatg cgcaaaatcg cctgggcatc
     3361 tgcctccgtg ctgatctcat cgcagaccag cacctccggc gtcagcacac ggagcgctgt
     3421 cagcagaccc tctgcccgtg gaaagccgtc cagcacatcg gtgtgcagtc ccacatcata
     3481 ctgcggcacg ccgtggtgta cggcggcgat ctcgctgcgc tcatccacca gtgtgagccg
     3541 ccaggatgcc cccagaaggc ggcaaaggtc acggagcatt gttgtcttgc ccgaccccac
     3601 agcgcctgcc agaaggaggc tggatggcct ctccgggcat atctgccgga agagcggctc
     3661 ggcacagccg gtcaccgcat gagagacccg aaagttcaga ccgctgatgt ggcgcatctg
     3721 caccagtttt ccctcccgat agactgccga cccggcaatg cccacccggt tgccgccggc
     3781 aatggtcaca aagccctcgg cgatctcttt cgcatagcgg tagacggaat attcgcacac
     3841 cgcctggaat gtctgctcga tagttctctg tgaaaccgga tgtcccgggt ctagaggctt
     3901 ttctctgctg tgcagcagaa tgcacgccgg ccgctctgca cgcaggcgta tctcctggat
     3961 ctgtgcctgc tctgacggcg tcagctgctg tatccggctg cgcacctgct gcggcagaca
     4021 acgcagtaca ttttgtatcc cttgctgttc catacgtcct cctgttggtc atgggctgtg
     4081 ccctgtgttt gtcccattct atgcacggcg gcggacgagt atgcaaaaaa cctccgtgcg
     4141 ccggttgggt gtccggtaca cggaggtttt ggggtattct attacaaata aaatctacag
     4201 cgtcagctgc atccggatat gcggaatacc atcttccaga aattcttccg atgtttggtg
     4261 aaaacctgct ttttcataga gacggcgtgc gtaagtctgc gcttcgatag tgattttatc
     4321 tgcaccaaat ttttcttttg caatattgac cgcagcagca acaattttac ttcccaatcc
     4381 gcaccggcgc tttactgcga tcacacgccc gattgcggca gaatcgaaag tcacacccgg
     4441 cggcagcacc cgtgcatagg ctttgattcc ggtttcatct ttgagccaca catgataagc
     4501 ggctctatct gcgtcatctg cctcctgata cggacaattt tgctccacaa cgaaaacaga
     4561 gatccgcagt ttatagatct caaataattc ttccgcactt aactcggaaa aatgttttac
     4621 aatcagttcc attctgtctc tccacaattc cactgtattt tacatatgct gccggcaata
     4681 cttatgcacc cagtcaaatg cgtcttcctt gcagtcggtt tcctctacat actgaaaata
     4741 tttttcggca agtgcatcat cagaaagccg ttccaatttg cttttttcac agacgttttt
     4801 tctcagcaga tactgctcgt atgcttctat ccagacaggc cctcttgcca taccgctgtc
     4861 atctccttat tttgccgatt acgaatcccg attctctatt gcatagacca cgcaggcaat
     4921 tccggcgatc atcgaaaaca agattgctcc acccattgcc tcgtccaaga ctcccccaaa
     4981 gaaaagaccg acgattccgg caaggcacat cacgataacg gatacaattg ttcgaaactt
     5041 tttcatgttt gttttcctcc gtcaattatt ttctcctgcc gcacgatgca ggttggaaat
     5101 tatagctatg aacgtccttt ttcactgctt ttaggtggac gaacatattg tagcatctct
     5161 tcttcacagg caccaaattt caaataattc gattttgctt catttagagt tattccatga
     5221 tttgatccgc tggggtcgtc attagaagca ataagcggat cattttccca aaaacaaaca
     5281 gggcaaataa atccccaatc ttccgccggc ggaacattat atgtaaaata tgaacagcac
     5341 ggacattgat attttttcaa gtacacgcac ctccacaact tgtaatttca cgcattggct
     5401 gacctctcca tgttcggcat tgtgatttgg atttcagaaa tacaaactga aatttctatt
     5461 tgcgctccca caccat
//
LOCUS       DAWENO010000140         5462 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5758_length_5462_cov_9.889773, whole genome shotgun sequence.
ACCESSION   DAWENO010000140 DAWENO010000000
VERSION     DAWENO010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_5462_cov_9.889773"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1162 a         1294 c         1307 g         1699 t
ORIGIN      
        1 aacctacata aggaactttt ccggaagcga gaacatcacc cgtatccaaa gaaaattgat
       61 atccatttgt aaaggcttca ttttctgaca aaaaagtaaa agctgttggc gaaacaacgt
      121 tgtaagactc ttctatatag catacacttt tatctgcctc tacatcgtaa ataccaatat
      181 tttcattctg aacattcagg taaagctttc cggtatacgg attcacccca tatgttttga
      241 atgcatctcg gtaatcgctt tcggataaat caaccgctgt ttctgtttta tttgtattca
      301 ggtcgtaata catgatttta gcttcgtctt gcatatagta gattctattg tttaccggat
      361 tatattgaat tttatatgtt ccatcatgtg tattaaaatg tgctaaagat tttactccat
      421 aagttgccgt attgccagct gttttctctg ttgtctgttc ctctgttgct tccgttgtgc
      481 actctgtctc tgtcgctttt tctgtggtct cttctttgga ttctgtttcc ggttcggtgg
      541 atgcttcgct gctctcttcg acttcggcga cgatggcagg cgtggatacc gtttgctccg
      601 gttcattttt gtggctcatc agataccaga tgaccaatcc ggcgagaagc agcagcagaa
      661 tcaggattgc gatcaggcag ccaagcggcg acttcttctt ctttttgacg gcagcatttt
      721 ccggatttag agtgccgtta ggattgtttt gaagtgcacc gtttgcattt tttggtgcaa
      781 tcgttccgtt gggataagcc tgtccgttcg gcaccggcgg gacaggatag ggctgtgcac
      841 cctgaaaggt agcgttcggt gcttgctgcc gaggattctg tccgtttgga tttgcagcag
      901 gattcggctg accggcgatt gcagcattcg gaggtgctgc agttccgggt gcagcagcag
      961 gtgaaaatgt tgcattttga cctgacggat tcgggcttgt ctgagaagca ttcgacactg
     1021 ccggctgctg tttctgtgtc ggcggtgcgg cggcttttgc agcgggcttc ggtgcagccg
     1081 gattcgctgc tgcattcaca gcccctgttt tttccgctgc cgctgcggga agtttccatt
     1141 gcagtgcgtc tgtaaaatac gtgacaactt cttcggcttt ctttttctct gtcgagaatt
     1201 cacgaatcag tctgccaatg gcctgctgta cggcagcctg ttttgctttt tcgtcctttt
     1261 cgtctgcatc gatcagaatc gaaccaatgt cgctgtcgat ggcgtttttc accagattgt
     1321 gattcggaaa atcattgccc atcaggtcat tgatataggc gttcattttg gatttgtttt
     1381 tcaggatttc ctttccgccc atttccagac agtaggcaat tccctccgaa accgaatcga
     1441 aatactggaa cttagagata aatttttctt tgcaatgggg acaatgctgc ttatctgaca
     1501 atttttcgtc aaacaagctc catgtcttat tgcatttgtt gcacttcata atcagattcc
     1561 tcttttctca catactgccg ccttttagcg acatgttgtt ttgtgttttt gtttctccgg
     1621 ataatccgca ccaggcacac gttgcatagg attccccatg gtagcaggta atcttggaac
     1681 agcgtggaca gtgtaaaaaa gttttggttc cgcaaaaggg acagccgtta taatcggggg
     1741 acgggtgaaa tgttccgttc aaaacggttt cgttgtagcc ctccgatttt gctttgctct
     1801 cgtcgatcgg gaacgcccat gtcagtgccc agtggtctgg ctgcttttcg actcggatgc
     1861 cgtataagcg ccggttgcac tgggggcagc gtgcaagaac aatgtgtgcc tctttgatcg
     1921 ccatgttttt cactcctctt cccattcgtt ttcgctttcg ctgatgatcc cttccggaac
     1981 tgcgtcattt acttcgccgg cattgccgct ttctgctgaa tccggttctg ctgcatccag
     2041 agagaattcc tctggctgtg cggatgcggt ttctgtttca caggcgggaa tgtgggcttc
     2101 ctgtgcatct tgctgcgctg tgatttcctg tgaccggtca tcctctgaaa ccgtgggaca
     2161 gattgcagcg ttgtcttgtg ttttctcctc tgtatcagca acttgcacct gaccggaagc
     2221 atcgtctttt tcttcccagc ctctttgcac tgctgcaata tcccgtgtga cacctgccag
     2281 cttctgctgc tgttcagaga gattttcctc gattttacgt ttcttttcca gataccgctt
     2341 gattttttcc tcggtttccg gcgtttcagc cgattgtgac aaaaggcgct gtatcttttc
     2401 cagctggaac tgcaattcct gaatggtgtc ctgcaaatgc tttttctgtg cagttagcgt
     2461 ctccaatttt gtgatctgtt gtaaggtttg ttcgatagcg gataacttct gcacatccag
     2521 aatccccgca atggaaacat cgtcatgact gccggatttg gagatgtttg caagattggc
     2581 ttgtagcatt tctgttcgct ttcggaaatt gtcgtgtaaa aaatcaagtg caacattgcg
     2641 gtaaaaagag tgcagcatct cggcaaatgt atcatcgata ccgtccgttc ctagaaaaat
     2701 ggcaacggga atctggttgt cccagatgtg gcagcgaaat tcactggctg catttttatc
     2761 gcaaatggaa gtgcagcggt tcaaatggca gttctcatcc catggaatcg gctcgtccat
     2821 ggtcccgtcc tcgtataggg caacgcattt cccatcgccg atgtgcagac cgatggcgta
     2881 ggatttgcat aacccgaccg caattagagt agtaccgtag gctttctgta cgtgagaatg
     2941 ctccttttgt atcaggtctg agatctcgct ttccgtatgt gctctgtagt tttcctcggt
     3001 gaaaaattga aaggttttct ggtacttttc cggcactctc tccagttcgt tcggcgtgaa
     3061 aggggatttg atcaggtcat ttctcacggc aatatgccat tccgtaatga tgctgtggat
     3121 cagctgtgtc accacacttt ttttcgtttt ggagtctgga aacggaaagg tgattgattt
     3181 tagaaattcc cgaatcttat tgcaagcgat ctctgcggca aagcgggaac ctcggtcgct
     3241 ccggaagtac tgcttgctgc cgtgaccgtc tgccacagtt gcaacgacac agtctgcatc
     3301 ctgcagatat tgtgtactgt cctggcatac ggtatggttc aagatatgct gatagccctt
     3361 ttctgaaacg gcaaatcctt cgtacatcat cattgtttaa tcccattcgt catcatcatc
     3421 ggaatcatcc gaacccagta tcatcggttc gggcgattct tcggtgcttt ctatactgct
     3481 gtatgcagat tccttttctt cttccagcat aacctttgcg gcttccgcca tctgatgttc
     3541 cgcagacgta gaacggacgt ccttcacatc tgccggtgct tccgcagaat cctcggaaac
     3601 cgttgcagag gtgcttgcga tctgtgagga ggtgactgca atgaaacgga tgatcttttt
     3661 gaggcttccg atgtcgttta cagtgaaaag aagttcgaca ttgcgaatcg cttttttcag
     3721 cacttcacgg ttggcatctt cgccgattgc gattgcaatt ttcaacgcag cctgatacca
     3781 cttattttgc tgtagctttt tcagtgcagt gaggtagtca tctgttggct cgccgtcaga
     3841 gaggagcagg atgacaggcg cataaaatcc ggaagatgct gcgcctcggt ccatgaatcc
     3901 atttttggac agcttgtcgt tcagctccag aaatgcagct cccagatcgg tcaggccgtt
     3961 ggcgtccagc ggttcccaat catcaaaggc gtccagagac atcagagcag gcgtgaccca
     4021 ttcacagccg gtggaaaatt tcaacacgct cagtttgatt tcggaatctg cctgcgattc
     4081 ggaaagatgc cgcagatccg gaatcagttc ctcgattgca gaattgacgg caccgatttt
     4141 tttgccctcc atgcttccgg aggtatcgat caggaaaaag atcggcatga ctcttttcgg
     4201 gatgctctct gcatttccca gaaaactgct tagattttca gacatagtct agtctcctct
     4261 tctgttattt gatttccata tactcatttt tgatttgaat gcggattccc ttttgaatca
     4321 gaacacgctc tccgggagcg acagtttcat tgttgatccg ccaagattcg ccggacttgt
     4381 tcatgagtgc aattttggtt ttggttttat tctgtacgac ctttgctgta actgtccggc
     4441 attcgttttg gtcgtagcaa tctgtgatgt aggctgtaac aatctcctgc ttggggaaaa
     4501 tcggaatgcg gaaatattgt gtctggatcc agccaatgtg cgtgattggc tgatgacagc
     4561 acatacacgt tgcattttca gtggtatatc tggtttcttt tccgcaattg ggacagatga
     4621 tgacttccga ctggaaccga agcagatttt gcagccactc cttttccgtg attcccattt
     4681 tgtcttcgca catgacttcc ttgctgaaag ccttgataaa ggtattctga atatataccg
     4741 gcatctgcgg ccatctcatc aggaagttat tgtgcacgcc ctttaccgga cggttggacg
     4801 cattcttggg gtcggcaatg aaaacagggg attcgccgta atagcgcttc tgtcgttgtt
     4861 ccgtcatgac ggccttgctc tgcgtctgct cgccctccag agggtggttt ctcagcagaa
     4921 gcaggaaaag gacaacggca agagaaaact ggtcggttct cttatccggc gtctttcgtc
     4981 ctacaacgat agatggtgcc atgtatctgc attttccagc aatcccggac ttttctccaa
     5041 actgggaaac gttgtcgtta tcgcagatca ggacatctcc ggtttcaggg ttcaggaaga
     5101 agttgccgtc attcaaatcc tgataggaga atccacggtt atgcagaatc cggaaactct
     5161 ctacaatgtt gatggctgca ttcagcagcg tgtgaacact ggaaaagtca atctttccga
     5221 tcaggaaatc agaaaagtca tagtagttat cggggcgcag tttcataatg tagccaaatg
     5281 tatcgtagta catacgggtg atggcaacgg gccacaggaa tgagtctgtg ggagcctgtt
     5341 cccggatgtt gttttgcagg ttcttgcgaa atccctccat gtcctttatc gaaccttttt
     5401 tgtaccattt caatgccatc tggtttccga gatagtccac cagatagaca tatccctggc
     5461 cg
//
LOCUS       DAWENO010000141         5449 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5773_length_5449_cov_9.254727, whole genome shotgun sequence.
ACCESSION   DAWENO010000141 DAWENO010000000
VERSION     DAWENO010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5773_length_5449_cov_9.254727"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1204 a         1505 c         1516 g         1224 t
ORIGIN      
        1 catcacatcc cggaactgcc ccaggaacat ccgcagagga tggagctttg ctgccgccgc
       61 cagttcgttt tcgccgtcct gtttcagacg ttcccgtgct gctttggttg tcagaccgtt
      121 cataggattc cttctttccc ggatttgttt ttgtgttttt gcttatgtat atgcggaaaa
      181 gccaaaaagc atgaccacgg tagaaatctt tttggcagaa tcacaagaaa atgcttgaca
      241 aatgtgccga aatcatgtat aatgtaaagg atgtgttgga aacgtgagaa tggaacaggc
      301 agttcccggt gaaccgacct gttttcgcaa gacaaggcaa tatagaaacg aggttgaaaa
      361 atgcactatc ccaagtggct ttccctgctg accctgggtg cggcggcagg cggaatgctc
      421 ttctttggcg gtctgaatgc gtctgcggcg gatgccgtac ccctggaatc ggctgccccg
      481 gctgtggcga cgtccacgct gccggaactc tcccctgcgc cggaggaaaa tccgggcggc
      541 tgggtcatga aagacggcgc aagaaagtat cagtacagcg acggctctta cgccatccag
      601 gcggcaacga tagacggtgt tctgtatctg ttcgactcca gcggcacgca gatgacgggc
      661 tggcagaaga tcgacgacac ctggcactat tatgacagcg ccacctatgc ggcggcggtg
      721 ggtgcgcaga agatcgacgg cgtgacctat ctgttcgact acaccggcgc acaaaagacc
      781 ggctggcgca ccgtagacgg ggtgcggcga tactacgacc cggagacggg aaagccggtg
      841 gaaggctggg tcagctacgg cggcagccgc tattatgtgg acgatgccgc aggcaaggtg
      901 accggagaat acacggtgga cggtgtccgc tatctgctgg acgaggagac cggcacccag
      961 aaactggggc tgtgcatctt cgatgaccag accgtccgct attacgatgc gaacggcgag
     1021 ctgacctccg gctgggcaga ggacggcgaa aaccgctatt atttcagcga gaactatgag
     1081 atgcagaccg gctggcagat gctggacggc aagcgctatt acttcggcac ggacggcatt
     1141 gcccgaaccg gcatccagac cgtggacggc aaacagtact gctttgcctc agacggctcc
     1201 atgcagactg ggttccaggc ggtgggcagc cagaagtact acttcggcac agacggcgca
     1261 atgctcaccg gctggcagac catcggcagc cagaagtact acttcctgga aaacggcaat
     1321 atggcgacgg gaacggtcac catcgacgga aagaagtaca ccttcaacgc agacggcacg
     1381 caaaagaaaa tcaagatctg tctggatgcc ggacactacg gcaaatacaa ccgcagtccg
     1441 gtgaacggta catactttga atccgacatg agctggaagc tccacctcaa gctgaaatcg
     1501 gcgctggagg cacggggcat ggaagtcatc accacccgga cgacccaggc aggcgacctg
     1561 gggctggagg cacggggcaa gaaatccgcc ggatgcgacc tgttcctcag cctgcacagc
     1621 aacgcctgca acagtgcatc tgtagacgca ccccttgcct gctgcaccgt gaccggcact
     1681 atggatgtac tggggcagca gctggcgaac gtggtacacc aggtgatggg gacagcacaa
     1741 gccggaacga tctggaaacg ccagggcgac aacggcgact attacggcgt tctccgtggc
     1801 gcaaccaagg tggggacacc ggcgatcctg ctggaacaca gctatcacac caacctgcga
     1861 gccaccaact ggctgctgtc agatgccaac ctgcaaaaaa tggcagaggc tgaggcggat
     1921 gtgatcgccg cattcttcgg actgctgtaa cagaatcaaa catcggcaca ctgggtagac
     1981 gatacctggt gtgctttttt tatggttggt atccggcaag catctttccc gtcttatcag
     2041 aagagccatt gcaacagaat atccagcctt tcatgcgaaa gcagtcgttg tccttgtttc
     2101 cggaaaaagc agccgcatgg ctgcacctct ggatataaca ataactctgt atctgcatct
     2161 gtctcttttt ctatctctga atctaactct aactcttact ctgactcttt ctctatctct
     2221 atctctgtat ctacaatgtt actcgacgct ggtaacattg agatcacaat gtgaatcaca
     2281 ttgtgatctc aatgtgattc gtccccactg acacaaaaaa agcagcgtac cggagatcaa
     2341 accgatacgc tgctttcaga actcgtcttt gcaagaaatg tatgtggatt ttcgagcatc
     2401 actttttgcg aaaacttgtt cttatataat gggaaaattc aagatgtcgt gattatgcct
     2461 gaaccggagt gccggaaact gccttttcgg aaacttccac ctcagactcc tgttccttct
     2521 tcgcacgctt gcggaaacca accagcagga gacctgcgaa tacgacagcg atcactgcca
     2581 gtaccacgca gtctgtggtg atgtccagac cggatgcgat gccgctgcgg aaaccgtgaa
     2641 cggtataggt gaacggcatg aacgggttca gaacctggta gaacttgtcg gacagttcca
     2701 gcggatatgt gccggcgcag ccgctgagct gcagtaccat gaataccagc aggataaagt
     2761 caccgatgat atccaggaag aagttgacac aatattccag cgtcaggaat gccacagatg
     2821 ccaggcaggc gatgatgagt gtcttgccca tatattccgg ctggaagtcg ttgatccagg
     2881 acaggcacag gatcatgaga actgcctgga gcagacccaa tgcccagagc ttgcccagtg
     2941 tgagcagcca tgccttgccc ggcttgcgag cgccgatctt ctgatgatac ggcgaaagca
     3001 taacgcagaa tgccagaccg gatacccaca gaccaacgga catcatgtac gccgccatag
     3061 cgttgccgtt tgtgggaacg gtcgtctcat atgtctcgtt tgtcattacc ggcgcagaga
     3121 acatatcata ggtcttgtcg gtgctgtcga tgctattgac ctcgtctgcg ccgtcggcga
     3181 tggaagtctg gagcgttacc gcaccgttgg acagctgtac cagaccgtca cccagttcta
     3241 ccgagccgtc tgccagctgg gatgcgccgt cctggatctg acctgcgccg tctgccagct
     3301 gtgctgcgcc gctgttcagc gtgtcgttgt ttgccgccag ctgtgcagta ccgtcagcca
     3361 gctgggatgc accgctctgc agctgagaaa ctgcattggt cagtgtagga acgctgtctg
     3421 ccagggtctg tgcgccgctg ttcagcgagg acgcaccgcc caccagcgtg ccggtgttgg
     3481 aatccagggt ctggagtccg tcggtcagac tggacacgcc gccggtgtac ttggatacac
     3541 catattccag agtgctgctg ccgtcagcca gctgactgag accagcctcc agtgcctgag
     3601 aaccagggat gagctgtgca tccactgcgg actgtaccga ctgtaagccg ccggacaggc
     3661 tggtgatcgc ctgctgtgcg cccggaagtg cctggtctgc gccggcagcc agtgtgctga
     3721 cgctggactt cagctgttcc attgcgctgg cagagctgct cagatctgtg ccttccagtg
     3781 ccttggacaa gccggtcacc tgtgtgccaa tgtccgagat atccgttgcc agagaactca
     3841 tggggacgga gaagcacgac agcagttcct gctgggatgc cgcatccaga gactggaata
     3901 cttccgtctg ggtcatggtg gtcagtgcgg actgtaagct ttgcagctgt gcgccggcat
     3961 cctgtgcgct ggtgccgatc gccgtcagac tggcggtcag cgtctgtacc agagcgctgg
     4021 tatccggcat ggaagtgttt tgcagggtgg tgttcagtgt gtcgatgccc tgctggagct
     4081 gtgtcagacc ggcggtcagc tgctgcatct gctggaggct ctctgcggag agactgccgc
     4141 tcagactgtc agacagggtc tgcaggccgg cagtcatctg tgcgctgccc tgctggagct
     4201 gtgccgcacc gctggtcagg cttgctgcgc cgttttccag ctggctgctg ctgccgtcca
     4261 gggtctgtgc gccgctgtag gctgccgaaa cgccgtcggt atagctgctg acaccgttgg
     4321 aaagtgtacc ggaaccgtca gacagtgcca ctgcgccgtc tgccagcgtg cctgcgccgc
     4381 tcgccagctg atccatgccg tcagacaatg tctgcgcacc gctgttgacc gttgccacgc
     4441 cgtcggtata ggttttcaga ccgctttcca gggtctttgc accgtctacg aaagtaaggg
     4501 agctgtccga caaaagcttc agattatcgg tgatcttggt gttgccgtcc ttcagatcgg
     4561 acacgccgtc ctggatctgg agcgcaccgt cagccgcatc gctcatgccg ctgccgatcg
     4621 tacccaactg gtcgaagatg gtctcggtat actccttggt cacgctctcc gaaacttctt
     4681 tctggatctt tgccagcgcc gtttcgctca tcttgcttgc gatgtagttc gtgccgggat
     4741 tggtgacata ctggagttcc attttctgcg gctcgtcgtc catcagcgtg gaggcgtttt
     4801 ccgagaagtt ttccggaatg gtgatgacca tataatatgt gccgtcctca attccctttt
     4861 ctgccttgtc tgcgctgaca aagtggaaat ccagctggtc attttcccgc agtttgtcta
     4921 ccagttcgtc tccgacagcc agttccttgc cctcatagtc caccgacttg tcattgttga
     4981 ccactgccac cggcagctga tccacattcc cgtaaggatc ccacatggaa cccaggaaca
     5041 aggtggtata gatggtcggg atcagaacaa ttgccgccat tgcgatgcca ttgaagtgcc
     5101 gccagaagaa attctgtttc tttccggaac cgcttgatcg cataaatacc tacttccttt
     5161 ctgcatgaaa caatcaccgc tttccccgaa tgcacggtct tgtttcgatt cttgtgacga
     5221 agaacagctg tctataaaaa aaatcttgtc tataaataga aaactgttga cttttcgtcc
     5281 tgtatctatt ataatataag ggaagtgaat ttgcaaccgt tgatttcaca aattacgtct
     5341 actaataaac actatgttta ttagtggctc cggcaatccc cgaatgccgg acagacgccg
     5401 cttcgtatac tattataatg gacagcaccc cacgagacct gtgccgtgc
//
LOCUS       DAWENO010000142         5438 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5785_length_5438_cov_9.175181, whole genome shotgun sequence.
ACCESSION   DAWENO010000142 DAWENO010000000
VERSION     DAWENO010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_5438_cov_9.175181"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1400..1409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2962..2971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1109 a         1428 c         1549 g         1332 t
ORIGIN      
        1 agattgtcca gaccgtagat gatgcggcag ccggcatctt ccaggcggcg ggaccattcg
       61 atgttgttct cctcgtcgaa tctggcacgg agttccacca gtaccacaac atccttaccg
      121 ttttcggcag cctcgatcag cgcctccaca attttgctgt tggatgcgac acgatacagg
      181 gtcatcttga tggagaccac ctggggatct cttccagcct cgttcagcag cgtcaggaac
      241 ggacggatgc tctcataggg gaacgccagg aagcgatcct tttcctcgac ctgttcgatc
      301 atgcgcttgg acgagtcacg ccatgccggt ttctgcggca cacggcgctg atagaacagg
      361 cacttgttgt ggcggagcat atcccgtacc accgagagaa cggagagatt cagcggcgcc
      421 tcagagtaga atacctgttc ctctgtcaga ttcagatagc cgcacagaga atggatgacg
      481 gcggtatcca tcagacggga gaattccagc ttgatggggc agagcttttt ccgttcccgg
      541 atcagctgct ccattgcctc acgatagtcg cattcttcct ccatgcgctc gtctggttcg
      601 atgtccgcat tgcggatgat tcggattagc gacttgctct tgatggtgtg ccgggagaag
      661 atctccggtg caaagtgcag gatcagctcc tctgccagga tgaaccggtt ctttccggtg
      721 ggcagggaga cgatcttcgg gaaaacgccg ctgctgcacg gcacaatgcc cagcttttcc
      781 gtgccttttt tctccagcac gacaacagcg taaatgtttt tgttttgcag gaaggggaat
      841 ggctgctttt tgccgatgat ctgcggagag agcagcggct cgatctcagc cttgaagtat
      901 gcctccagcc atgctgcctc ctcggtggtg atggtgtgga agtctacgat gtccacgccc
      961 tgcatactca gttctgcacg caggtcagcg taagcgtcgt ccttgtcccg gagcagttcc
     1021 cgcactctgg cgtagatggc ggtcagctgt tccttgcagg tcatattggt cttgttttcc
     1081 cggaagtggg gatcttcgca catctggtca tacagcgagc cgcagcgcac cataaagaat
     1141 tcgtccagat tgctttggaa aatggagagg aaagaaagac gctcgcagag cggattggtc
     1201 tcgtcctttg cctcttccag cactcgctga ttgaacttca gccaggataa ttcccggtta
     1261 tcataacagt ttgatttcat tgctgattcc tccgatttgt ggtttgtttt tccggaaatc
     1321 ttccgaaagg atctattgct gtgaccggaa cagaccgccg gagcgtgtcc ggatgttctg
     1381 ttccggttcg ggatgattcn nnnnnnnnna aacaatgttg tataataata ctaaccaatt
     1441 attttttcag gacagccgtg ctgtctgaac taggagtgaa agtatgaaaa aatatgcagt
     1501 gatcgatatt ggttccaata cggttcgcat ggtgatctat acggcagagg acaccgcatt
     1561 tcacgggctg ttttccaaga agtacacgct gggactggct gggtatatcc aggacggcgt
     1621 catgtcccag gagggcatcc ataagctgtg cggtgttctg ctggaatgcc ggacaatgct
     1681 ggaacagttt gatgtggaac gctattttgc atttgccacc gcatccctgc ggaacattca
     1741 gaacacccgt gaggcagtag accaggtatt tctggtgaca gggttttcca tcgacgtgat
     1801 ctccggtcag gcggaggcgt atctggatta ctacggcgtg atggtggacg caccgctgga
     1861 aaacggtctg ctcttcgaca tcggcggcgg cagtacagaa ctggtgacca ttgcgcacga
     1921 tggtccgggc gtgatcgaga gtctgcccat cggttcgctg acgctggcaa aggagtatgt
     1981 ggacaaggtt ttcccgaaac agagcgagtg cgagaagatc caggcacgca tccggaaaga
     2041 actgaaaaag cgcaagctgc accgtctccc ggcacataca gcgctgtgcg gcgtaggcgg
     2101 cacggcgaga gccttgctga tgttggcaca ggaacagcag gagctgccgg acacccagcg
     2161 gcttttgacg gtgagccagc tgaaaaagct ggaaaaggcg ctatggaaaa aggacggcaa
     2221 ggcacgggaa ttgctgctga aaacctgtcc cgaccggctg cacaccatct atccgggaat
     2281 gctcatcctg gacgagctgg tgcagctgtc ccagtgtgag accatctata tcagcaaata
     2341 cggcgttcgg gagggatacc tccgcagaga actggcacgg aaaaagaaaa ccacagaacc
     2401 ggaaaaataa ggacaatatc acaaaagccc ggctgcatca tcgggacgca gccgggcttt
     2461 ttcgtttgtt atagagtgca gatgcttatg cgctttccac aggcagctgg ttttccagat
     2521 gcaccaggaa ccgcatcaat accacggcat cattgccgga aaggtcgctg tttgcatcac
     2581 agtctgcatt cttggttgcc tgtgcattca gcgaaacgga gccgcttacg gcacggttca
     2641 gcagtaccgc atctgtaatg tccactctgc cgtccaggtt ggtgtcacca tacagaacgt
     2701 cagctgtgcc ggtgtcagaa ccgctgccgg aaaccgaggc ggaagtgaca gtcgttgtgg
     2761 tggtactctg catcgtggtg gttgtctcag acggcttggt ctctgtggtg tcagagggtg
     2821 ttgtgccgtc cggctcttcg ccccagacca gctcgccgtt ggcataagcc gggatctttt
     2881 tggtgatggc tgcggcgtcg ttgtcgtcct caaagctgat gaggtcgccg tagctgtagt
     2941 cgttggagga atcccatgtg gnnnnnnnnn nagcagtccg aactggtata cccggctgcc
     3001 gtagaaattg cagttttccc atgcgatttc gatataatag ttgcccttgt cgtcgtattt
     3061 gatcggctca gaaatggtgg cgctggtctt gttgtttgtc atggcgcttt ctgcgtcata
     3121 gtccacacgg gtctctacca tggagatatc ttcgccgatg gcagtcagtt cgtcgatgtt
     3181 gaagaagtac cgtactttca gatcgtcaca gaactgcggc ggattgatgg tgcggttgtg
     3241 aatgacgatc ttgacctgca gaccggcttt gttttcctgt gccttgccgg cggtgacgta
     3301 gaatccggtc ggggtgtttt cgtccacttc atagcctgcc ttggcatttt cggacatatt
     3361 gaaatccgga atgaccgcat tcttgtctgc cagttccttg ccctctgtgc cgtagaagtg
     3421 gtacagtccg gcgaggtcgc cgcagaatcc ggcgttgtag tcgtccgtga cttcattata
     3481 aatgtagtcg ctggtcacat cgttgtgata gtccttcaga tccggaccgc cgaccagtgc
     3541 gccccacagg gtgtgcgtct gcggatcggg ttcgtccatg ctctgggtgg aggagcagga
     3601 ggaggaacgg tggtggggat gtgtggcata gctgttctcg tatcccactt caaaggaata
     3661 gcccatgggg ttgtcgccca ggatgtattc catctgatcc tttgcccact cagcgaaagt
     3721 cagatcgttt gtctccttgg cgtagaccat agcgcacaga ccggcagccg tgttgtaacg
     3781 ggcgctgccg tagtcggaaa caacagcaaa accggcaggg gacttggcaa tccatgcgcc
     3841 gtccgtctcc ggcagatcct tgatctccga ggcagtgatc ttggtgttcc acagctggtc
     3901 gtccatgccg aagtacttgt gcttggtgat cggaacgtcg aagtccgcat tggcggcatc
     3961 tgcctcgctc agaccggacc agaattccag gttccagcgg aaaatgtacc agtcacggga
     4021 gatattcgtc accggagcaa gctttgcgaa tacaccgccc caaacggtat cccagcagtg
     4081 tacccagatg ttctgccagc agttgccggt gtctttgatg atacgcttca tatagccggt
     4141 gtacgggtgc gcacccattt cgccggtaac gctttcgtca acgctgatga tatcgtcaat
     4201 gtagtcatag ttttcggtgc agtagtacag ccataccgct gcccacgcca gttcgtcata
     4261 gtcatagctg gaggtgtaga aaccgccgtc atagcccagg ctgtctacgg tctgggtgtc
     4321 gtcgcccact tcgtggtgcg tctttacgga gaaatcgtac agcgccaggg cgtagtccag
     4381 gcacttctgc gcatattccg ggtcagtgtc cttgaagttc agatagttca ctgccagaga
     4441 tgcggctgca ccggcggtct ggtcggatgc cggcgtttcg gtggttgcga agtatgccgg
     4501 acgcttgacg gcgcaggaag aggtggcaac ataggtgtcg tctacctgca attccggtgc
     4561 gcaccagtag ttgtggtcgt tgttgccttc gcccacctgg tagcagtatg ccactacatt
     4621 tccgtcgtca tctaggaaag tggcttttaa gaagtagtcg ttgatccagc gcagctcatc
     4681 ctccacgtgt ttctgcaagc cggtttcaac gtaagattcc cggaactcat aatagcccca
     4741 gcccacggta gaggcagagt agctgcccgg cagaccgaac ttgacgtggt caccggcatc
     4801 gtgccagcct ccggtcagat cgacgcagcc gtcgccgtcc ggatccagat atttcttgtt
     4861 ggcgtcaatg aattcctggc tcatgttggt gccgacatag agaccctctg ccgcagatgc
     4921 gccgccgtca cccttgccgc tgtcctcgtt ggctttgccg ccgcccaggc tctgcatggg
     4981 aaccagaggg atcatgccgt ccttgacgtg acagttgccg cgccaggtgt aatagccgtc
     5041 atctgccacc tcgtcgccgc acttattggc atcgtagaaa cacagcgcca gctggagtgc
     5101 ctcggcgaag ttgtcgtagt tgttgggatt ttccttgtcc acaacagccg cactgaccgg
     5161 cgagaccgca gctgatgcga tcattacgcc gctcaggagt ccggcggaaa cacgccggag
     5221 aagttttcga atcatttttt tacacattcc tttctgcgaa tttgttcccg tcctgccgca
     5281 gttgctttcc tgtgacagca taccgggagg ttttttgatt tcctatctga taaccgtgcc
     5341 atgccggaca cggacgtatc cattattttt cttctgtttt ttcggttcgg tcgaagtgga
     5401 tctgatcgcc ggcacagccg tgggtgctgg cacggttg
//
LOCUS       DAWENO010000143         5324 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5918_length_5324_cov_12.843234, whole genome shotgun sequence.
ACCESSION   DAWENO010000143 DAWENO010000000
VERSION     DAWENO010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5918_length_5324_cov_12.843234"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2012..2021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1282 a         1459 c         1328 g         1245 t
ORIGIN      
        1 atttacggcg gctgctgcga tatcttgccc tggacaccgc actggttctc gcagcaatac
       61 ttctgaccca cctggggaaa catcattttc ccgcagtaga cagtgccggc ggcgtttctc
      121 ttcccgtatt gatgtaccac agtgttgcgc ctgtgccgga acagcagtac tgtgtcacgc
      181 cggaaacgct ggagtccgat ctgcgctatc tccaggcgca cggatacgag accgtatcac
      241 cggaagaact ggtcgcatat accaacggaa acggcacact tccggaaaag ccggtcatgc
      301 tgacgtttga cgatggtctg tacaacaacc tcagcctagt gctgccgctt ttggagacct
      361 atgatatgtg cgctgtggtc tctgtggtcg gaatattcac cgacgaatac gctcccgatg
      421 cgccgcacaa cgatgtctat tcctatctca cctgggaaga cctgaaacaa atgcacgatt
      481 ccggacgcat tacgctcggc agtcatacct acgatatgca cgaaaacgga acacggcagg
      541 gctgcgccaa gacagactgg gaaacagaag aggaatatca caaaacactc tatgccgatc
      601 tgtcgctgat gcagacccgg tttcaggagg aactgcagat ccagccggct gttttcgcat
      661 acccctatgg ctttgtttgc agcgaaagca aaccggttct ggcagacctg gggtttcaga
      721 tcacgctcac ctgtctggaa aagccgaatt tcatcacccg aaatccagcc tgtctctatg
      781 ggatcaaccg ctataaccgt cccggaaaca ccagcacagc tgagatcatg caaaaagtgc
      841 tgtcagacga aaaaacagga ggtcttgcat catgcgcaga cgttatcaca agcgccgctg
      901 gacgctggtt tgctttttca ccgttatgct gattctgcct gccgctgctg tcattcccaa
      961 aagcggcggc agatcctccg cctatcccta tgcaaaagag acaggcacat cccctgctga
     1021 tgccacctgc tggaatgtgg catccgacgg ttacggctgg gaggaagagt cggcagatgt
     1081 gccggaaact gccgatgcag tcaccgaagt gacttcgtct gaccccggag aaaagcccta
     1141 tcccacggac tgggatctca gcagtgacac ggcaattcga cgcaccagct acggcactta
     1201 ttccggagaa cgttttttca gtctcttcac cgccggacag gtacagaaca aaaccagcat
     1261 tcccaacaat acactgatcg cagaaagcac tgctgcgcca ggttttcaga tcgaaacaaa
     1321 cggcgagccg caggtactca tcatgcacac ccacacaaca gaaacctttg agccgtttgt
     1381 ccgggaacag tttgacccaa acttcaatta ccgcaccaca gacccggcgt ataatatggt
     1441 ctcagtgggg aatgctatca ctgcacagct ggaatctgcc ggcatcggtg tgattcacaa
     1501 cacgactata cacgactacc cctcatacaa cggctcgtat gaccgcagcg cagaaaccgt
     1561 aaagtcgatt ctggcacagt acccgtccat tcgtgtggtg ctggacattc accgggatgc
     1621 gatccagagc ggcaatacgc tgacgcagcc ggtagtggaa atcaacggca aggagtctgc
     1681 acaggtcatg attatctccg gctgtgacga cggcactatg aatatgccga actatatgca
     1741 gaactatcac tttgcctgcc gtctccagga aaacatggag ggaatgtttc cggggctgac
     1801 acgccccatc ctgttcgact accggcatta taaccaggat ctgacaacag gcagcctgct
     1861 cctggaggtc ggaacacatg ggaacacgct ggaacaggca cagtatgccg gcgaattggt
     1921 cgggaaggca ctttccaaaa cactgctcaa gctgaaagcg gaggaatgat acatgaaacg
     1981 aacgacatcc cggatactcc gcatgctgct cnnnnnnnnn ntgctctgct atttgctgtt
     2041 ctgtggattt gtctgggcat ttctgacggt tcagatgcgt tcacacaacc ggctttccca
     2101 cgagccggca gccatggcac agctggtctg ttgcggcgaa accgtggcgc tgcatttgcc
     2161 ggatcagtcc tatcaatgga aactgccgca gctggattcg cagtggctgt ggatcgcagc
     2221 actgccggat gcagcatgga atagtgccgt ctgcacctgg caggttctga cggaatgttt
     2281 tctatgaaaa ttttttgtga acttctcaga tattccgggt tctgctcttg caatttcgga
     2341 tgagatatgc tataatagaa aagtcgtcag atgagtgtgt gaagtttttg catctcactg
     2401 ttgtcgatgc gcctgtagct cagctggata gagtgtcaga ctccgactct gaaggtcgtg
     2461 cgttcgaatc gcatcaggcg taccaagtga aaacctaccc gaaaatattg tttccgggta
     2521 ggttttattt tttagaaagg aagtttttgc aaatgcagaa tcagaacatc gacagcggaa
     2581 ctgtctttga ctggggaaga acttcggatg attatgcaaa attccgggac atctatcccc
     2641 ttgcctttta tgagaagatc ctggaaagga actgcggtac agccgggcag caggtcctgg
     2701 acatcggcac aggcacgggt gttctcccac ggaatctata tgccgccggc gcgcactgga
     2761 ccgggagcga cattgcgccg aaccagatcg cacaggcaaa agcgtgttcc gcaggaatgg
     2821 acatcgccta cctcgtatct gcggcagagg atctggactt accggcaggc tctttcgact
     2881 gcattaccgc ctgccagtgc tactggtatt ttgacaacgc cagaattgca ccggtacttt
     2941 cccgcctgct caagccgcat ggcaaagtgc tgtttctgtg catggaatgg ctgccttatg
     3001 aggacaagat cgctgcggca agtgaaaatc tggttttgca gtacaatcca aagtggagcg
     3061 gcgcaggaga aaccatgcac cccattgcag ttgcaccgga actgctggaa tacttcgacc
     3121 taacctatca cgaggaatac cttctggatg tgccgtttac ccgtgacagc tggaacggca
     3181 gaatgaaggc ctgccgtggc atcggcgcta cgctctctcc ggaagagatc gccgcctggg
     3241 aaaaggaaca tcttcagctg ctgcgcacaa ttgccccgga agcattcacg gtaaagcact
     3301 atgcggcaat tgcggaactg acaaagaaag aacacacgcc atgcacctga tacaagccaa
     3361 aagcatttta tctgccggaa acggcatgaa cctctatcgt ggctgcaccc acggctgcat
     3421 ttactgcgac agccgttctt cctgttatca gatgtcacac gacttcgagg atattgctgt
     3481 aaagggaaat gcgcccatgc tcctggaaga ggctctgcga aaaaagcgcc agctctgcat
     3541 gatcggcact ggctctatgt gcgaccctta tctgcatctg gaagaacagc tgcggctgac
     3601 acggcgctgt ctggagatca tcgaaaaata cggctttgga ctgagtattc tgacaaagtc
     3661 tgaccggatt ttgcgagatg tggacttgct ttgccgtatt caagagaacg ccaagtgcgt
     3721 tgtgcaaatg acgcttacta catatgatga aaaactctgc cgcattttag aaccgcacgt
     3781 ctgcaccacc aggcagcggt atcatgcgct gaaagtcatg cagagccggc atattcccac
     3841 ggttgtctgg ctctcgccga ttctcccctt cctcaacgat acggaacaaa atttgcgtgg
     3901 cattctggaa tattgctttg atgctggcgt caaaggcatt ctgtgctttg gaatgggcgt
     3961 aacgctccgg gagggtgacc gagaatattt ttacgcccag ctggacaagt acttccctgg
     4021 catgaaacag cagtacatcc gttcctttgg aaacgcttat gaatgcagca gccccaatca
     4081 gcgaaacctc atgtctattt tccacgagga atgccgccga cacggtgtcc tgcacgagcc
     4141 ggaacaaatc tttcactatc tgcgggaatt tccggagaaa acgcagcaat tgtcgctctt
     4201 ttcttagaat ctctcttcaa tcaaaaagcc atttccggtt ttgattcccg gaaatggctt
     4261 tttgtatttt tattttccag tcggcgcttt ggtttccagc cagcgcagca tttcatggaa
     4321 tccgtctttg gtcatgggga acttctgttc ctcttccatt tctgccaggt cagaacagag
     4381 ccggctgtgc cagatctgca cggtgaagtc ctcacccggt gtgatcttat agctgagttt
     4441 tccatagctc ccggtaaaat tgtttccgaa ttcgaaatag ctgtactgcg gaacgtcata
     4501 aacttctgat gccggatctt tacgcatttt tatcttctcc tctccgatga caaacaggtt
     4561 tcaagagttc tgtcacttct tccagatttt ctgcaatacc aaataggcgt tccctatctc
     4621 ccggttcggg cggtgtcaga tccggaacca ggatgacatt gcatccggcg tccagacctg
     4681 ccgctatgcc attgggagaa tcctcgacgg caacacattc ctccggcgga atccccagcg
     4741 tctttgcagc aatcacgtag atatccggat ctggtttgcc gtgttttacc tggtcaccgc
     4801 cgacgatgtc cgggaacatc ccgtccagtc cggcaagcgc cagccgctct cttgccagtt
     4861 cctgcgaagt tgcagttgca accgcatagg cgagtccggc ttgcttgcag aattccagca
     4921 gtgcagcaat gccgggcttt tgctccacac catgcgccgc aatgtactgg gaaaccaatt
     4981 cccggcggcg gtcacgcacc ttataatagt caaaatctgt tccgtacagc tgacggaagt
     5041 atggttctgc atgagccgca cagcagctcc gcacgtgcag cgccgcctct tctgacatgg
     5101 gaaagcccag ttcctttgca ctttgcagcc agcactggaa atagattttt tccgtatcca
     5161 gcagtgtgcc atccatatca aagatcaagc cacggatttt cttcactgct cctgtcctcc
     5221 cactgcaacc agcatctgcc gcaacagtgt cagatcaaat ccgttcacaa tgccgtcctc
     5281 ggtgacatct gcacattcat aggcagtttt cgaaatgcct tcac
//
LOCUS       DAWENO010000144         5321 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_5924_length_5321_cov_12.678883, whole genome shotgun sequence.
ACCESSION   DAWENO010000144 DAWENO010000000
VERSION     DAWENO010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_5321_cov_12.678883"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1678 a         1038 c         1130 g         1475 t
ORIGIN      
        1 acttttccaa agcgcttcct tgctatattt gaatatatta ttttagcatt tgcagcggaa
       61 ttgattgtat ctgtagtaac aaaaaacgcc tatatagaac ccctccaaaa atatgaatat
      121 gaaaacgaaa ttggaatctt cttatcccgt atggtaatct tttgggttgt actgctatta
      181 ggatattttt ctcatcacag aaatgagcaa aagattctcc ccatatggac aatgtttgtt
      241 tctccgtgca taccaatcct gacaattggc gtcgaattgc tgtttacaac tgtttctgga
      301 gtgacagcac aatcagtcac agtttccatg attctcttat ttatcatcaa tattgtagta
      361 ttttttctgt atgaatcact gtcaattgca tatgcacggg ctgcaaaagc aacagcggca
      421 gagcaagaaa aaaattacta ccagaaccag tgtcagctga tgcagaatgc agtggaagac
      481 accagaagat ttcaccatga catttccaat catttttcta ttgtagaaac ttttcttgaa
      541 gatcacaaaa ccaacgaggc ttcgcaatat ctacaggagt tgatacagaa ggaagaaaaa
      601 aagtctgtat tgtattctac ttcaggaaat attgtggtag acagcattat caattacaag
      661 ttaagatccg tttctgattg gaatgttgct gtaaccgtag acattgttat accaacagaa
      721 cttcccatag aaattgtaga cctttccaca attctgacaa atctgctgga caatgcagta
      781 caggcacttc aaaaaaccga cggaaaacgt gagttgaaaa tcgcaatgac ttatcgaaag
      841 ggtatgctct ttgttgctgt aaaaaacacc tatccaggaa aggttcatta tgaaaatggc
      901 gaaatcgtaa caacaaaaga agatacagaa tcacatggat atggattgaa aaatgtagaa
      961 tcagcagctg aaaaatatga tggaatctgt caactccacc ataatgatgt ctattttgat
     1021 gccgaagtag cactttattt gtaagaaaat gcggccaata aataaagaca aaatatgaaa
     1081 aactgtaagt ttcgtcgaaa acactgtaag tttcgtagac ttctcgcaca ggtacttttt
     1141 ttatggtata atagcaatac cgaaaagata atctgtgaag ggaggtcttt tttatgcgga
     1201 agttcttttc aaaatactgc ggtttactcg catctcttgc acttgctttc acaattgtta
     1261 cagcgaattc tacttacgtc ataagtattg atttccgtcg cccaattttg ctatactaaa
     1321 gtaaaacaaa aaccgagtgt ttcacactca cacccgctat acaaaacaac aggaggatat
     1381 ttttatgaaa aaacaaatcg caacactctt agccctgtcc actgccgcaa cgtgcatact
     1441 gacctatcag ccgacagcag tcggagcaga agaatactct gttctggact atacggataa
     1501 gctggtacag gaggcattgg aagacaacac ggacgagtta agagatgcac tttatgattc
     1561 ggtgacaggg ggagaaatcc cgctttacga agcggatgcg tcatcggcgc aaagcgagtt
     1621 ggaaaaggct gcgatcgaga agggaatgta caaagatact ttttcatttg tgaatcacct
     1681 gtgtacttta tcgcaagaag attttacaga aagagtatac gatccagtaa gggataatat
     1741 gacggaatcg gaaataaatt caggttacgg taaacatatt cccgatatat cttataaaag
     1801 cggtctttgt ttcggtatgg ctgccatttc ggtattggtc cacaatggcg aactgactcc
     1861 gagcgatttg caagagggtg cagaaacatt atacgatgta acgctgactg atgatgtgga
     1921 tgcgttgatt acttgttaca actctctgca gctatataaa gcggtggaac tcgcaattat
     1981 tgcagcacct tcgatgctgt ccaaggaaga gcatacggat atgtttctgg attgtgcagc
     2041 acgctgcaag gaaaaaggca cttactttat ggctggtatt gctaccaaaa aaggcggcac
     2101 acacgctgtc gttggcatgg atgaactgtc cggtaactgg acattcgacg gcatcagtta
     2161 tgacacctgc attgtcactt atgacagcaa ttgtgtgaag caaggtacag aaacatcggc
     2221 ttttaaggat gatgcctgca tctatatcaa ttcggagacc aaacagttct gcatcccggc
     2281 atatgaagcc tcttcggaga atggtgatgt cttactgtat gcctcagatg atgattctct
     2341 gctgacttat aaggcaccga tccgtggtac tacaaaaact aacactgatg tttcagaaat
     2401 cgtgaaatta gaattcttca atggcagaaa agatcagatg gatctaagtt ctaccacaga
     2461 ggatggacag acgtacgatt tttggaaatt aggcaaagtc aattacggag agtatatttt
     2521 ctttggcaaa ggttcttctt tccacttgga aaagaatgaa cgtgcgtcgg gatttgcttt
     2581 tagtattgac ggtgaaggtt atcgtctcag aatggaacaa tatgatcggg aggatcctga
     2641 actaaaaggt gttggttcaa agtgcaaaat cgatttctct aaagatggcg tgacctatac
     2701 caatacagat acacaaaaaa tcgcattatc atatattttt tcatacgatg aaggcaatta
     2761 caatttcatg ccagtagcca gcagtactat tgatattgaa gtcttaccga atcaaacggt
     2821 gacggtttca aaacaggata ctggatttgc aatcactggt gatggagaag tatatgtaca
     2881 ggcatttcca attgcctcac aaaaagagca agcttcattt ggcggcagcc atagctacga
     2941 agaatacctc gactggtttg gcagttatta cattaatttt atccgatcga aaaatgttct
     3001 gctgcaattc aataccgaaa aagggtgcag tgaactggta tatgactttg acagcgacgg
     3061 tgtgtttgac gatgctccta tgctcggcga tgcagacgga aatggaaaac catatgaagt
     3121 caatgacatc tactggacca tgctgcatat tgctgataaa gcagttgcac ctcacagtgt
     3181 aaggcggtat tataggggct atgttcccaa tattgaggct gatacagaca acgacggcaa
     3241 cattgacacc agcgacttgt tcgacattat gtacgagtgc gctctgcgtg gagcaggact
     3301 gaaaaaatag agatagtcct gttcttgaaa atgaaaaagt gacttttgct ttgctgattt
     3361 atcaggagag caaaagaaag gagacaactt aaaaatcatt acatatccga aagggaggta
     3421 ctacttatga agtctgtaaa aaaaattatt gctgctttat ctgcagctac aatggtatct
     3481 gcttgcgcag gcgttgttgc aagcgcagct gaggcagtaa acgcagtcaa tgtttcctac
     3541 agcactgtag cagaaacatt caccgccgct gacggcacag ttattccgga aggtgcaacc
     3601 gcagtaacac tgtccatcga gaacaacacc ggtttttcag catctgacat cacgctgaac
     3661 gcaaccgcag acctgttggc tgtggacggt atggttgccg caacaaacgg cagtgcttat
     3721 ggtgatgcta tcgtatctgc tgtacagaac ggcagcaagg ttgttatcac cagtgcatct
     3781 ctggacgatt ccaaggatga cggcactctg gtaaccttct atactaccag tgacgcagaa
     3841 gtaaccgtag aagatgcctc gttcgaatct gtaaagaaca atgagatcag ctgctatacc
     3901 agtcagcagt atgattttta cgtaattggc gatttgaaca gcgatgaaca ggtcaactcc
     3961 aaggatgctg cgatcatgct gtctgcatac aatatcgcag aggctgacgg cagcctgctg
     4021 acaacagcaa acggaaaagc tctgatggtt tctattgcaa agatgaatcc ggcaaaatat
     4081 aaactggact ccgcaaaaaa agtagaggca gcagatggaa acaaggacgg cagaatcatg
     4141 aactgcgatg atgtaaatta ttctgatgta aaacagctcc tgaactacgc cgcagctact
     4201 ggtgcaggtt tgacttatga tgtggcaggc tggcattacg gtgagtatgt gggatacgtg
     4261 aagtaagccc atattttaca aacttatgcc atgttagaca gacaaatcat tatgacagaa
     4321 ggacaagttc gttttttcaa ttcgaggttg tccttttgta gtattttgag gtttatggag
     4381 aataaggagg atgttgtatg tctttagaaa tatatcaagc aattgttgat actgcttcag
     4441 cagtaggaag tctagtggca gtctgtttag cgtacaaagc ttttgacgtt tccagaaata
     4501 tatccgatca tgagcaagat aatgaacgaa ctcgcctgac aatagatgca ctaaacaagc
     4561 tgcaagatga ggtcctgagc aaaatatatt gtcctgaact ctatgaaatc aaatcagatc
     4621 tacaagaaaa agaatataaa atcatgctct ctaaattcga attttttgca gttgctgtaa
     4681 aaaataaggt ttatagcgag gaactggtaa tcaaatgtgc cagtgaatca attttctgga
     4741 cgtttagcaa gctgttcccg ttcatgatcg gaaagctaag acaagcagct gatcgctttt
     4801 ctgcatctct atggctcgcc gaaagatgta ttcaagggaa tgctaaaaaa caaaaaaaat
     4861 tccaggaaat gatgtccaaa gtcgcattgc aaactcattc agaagcatat tgaaatatag
     4921 cgtatttgga atcccattct ctcataaaac agcacgccaa gaacccacta tttatagttt
     4981 tggcgtgctt gttttgtcat ataaagtctt cctccgcaag taattcagcc tgtgcaatgt
     5041 tccctctagt ccaccacata tttcgtaagt ctgccttatt tacatagatt ctcttgaatt
     5101 tagtactgct gtcgtccaaa agcatatacc ccctttcttt ctctttcttt tactttcttt
     5161 cgtttctttc acaaatatag cataagaaat agaaaaagtc aagtatttcc cctgcggtca
     5221 atcttatgat ttcacgctgg aatcgattct atacagaaaa aaagcatcat gacgccaact
     5281 gctcacaatg cctgctcaaa aactttcggt gcatttcact t
//
LOCUS       DAWENO010000145         5195 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6086_length_5195_cov_9.664981, whole genome shotgun sequence.
ACCESSION   DAWENO010000145 DAWENO010000000
VERSION     DAWENO010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_5195_cov_9.664981"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1289 a         1311 c         1532 g         1063 t
ORIGIN      
        1 aaaaccgaca ctttatcctt ggccccggag acgctggggc agtatctggc ggaaatgggc
       61 gagccgaaat tccgggcaaa acagcttttt tcctggctcc atcagaaaca ggtgacggat
      121 ttcgcccaaa tgactacact ttctctccaa ctgcggcaga aactgaatga aaaattttgt
      181 ataaatagac taaatattgc aagaaagctt gcatcttctg ccgatgatac ggtaaaatac
      241 ttatatgaac tgccggacgg caactttgtg gagacggttc tcatggacta tcaccatgga
      301 aaaagcctct gcatctccac ccaggtgggc tgccggatgg gatgtcagtt ctgcgcatcc
      361 accatcgccg gctacgtccg ggacttgcag ccgtctgaac ttctgctgca aatttatgag
      421 acccagcggg atgcgggatg ccggatcgac agcattgtgc tgatgggcat tggagagccg
      481 ctggacaact ttgacaacgt ggtgcagttt ttccggctcc tgtcccatcc ggacggcatg
      541 aacatgagcc tgcgccatgc ggcgctctcc acctgcggac ttgtgccgca gatccgtcag
      601 ctggcagacc tgcggctggg cgtgacgctt tccgtctccc tgcacagccc ggagaatcag
      661 cgccgcagcg agatcatgcc ggtgaaccgg cggtatccgg tggaggagct gctggatgcc
      721 tgccggtact atttcgatac caccggcaga cgtgtgacca ttgagtatgc cgtcatcaac
      781 ggtgtcaaca acaccagggc agatgccggc aagctggcga aacggctgca cggaatgcag
      841 tgccatgtga atctgatccc ggtaaatccc atcaaggaac gctcgttccg gtcgttccgg
      901 aaggatgtgg agcggtttca gaaggaactg acggcactgg gcatcaacgc cactatccgc
      961 cgtaccctgg gcagcgatat ccaggcggcg tgcggacagc tgcggcggga tgccgacgca
     1021 cagagaaacg ctgtgcagga atccacttaa ggcagcactg cacaaagcct gtctgacggc
     1081 tttgtatgca atagcacaag ctttgtgcgg tgttgtctta acttttcaaa gcttttttcc
     1141 ggaacgcagc cccggacagt ccggcggcag tgcgttctgt aacagggaac ctttacggag
     1201 gtggtacaat tgacgagtgc atcgcaaacc catatcggtt atgtccggga cgagaatcag
     1261 gatcgtgtcg ggattcgtca gtatggagaa aatctgctgc tggtcgtctg cgacggcatg
     1321 ggcggtgaac gttccggcag caaggcaagc tccatggcga ccgatgtctt tttcgaacat
     1381 tttgaggaga gctacacaga ggatatggat gcctacagca tccggtctct gctgctgtcc
     1441 tctgtctctg cggcgaattc cgtggtgtat acgaccgcaa ggatggacta tcagaacttc
     1501 ggcatgggaa cgacctgtgt tgcggctttt gttgcgccgg aatatgtggc agtggcaaat
     1561 gtgggcgaca gccgggcgta ttttctcacc ggcggcagga tggaacaggt gacgacggat
     1621 cacacggtcg tgcatatgct catggaaaag ggcgagatca cagaagaaga gatggagacc
     1681 catcctcagc gacatatgct gatgaaggcg gtgggcgtag aacggacggt ctgcccggac
     1741 tttttccgca ttgatctgga aaaagaccag gcgttccggc tgctgctctg ctcggacgga
     1801 ctttccggtt tctgctcgga tgcagagatc gaaacgattc tgaaagagga tctctcggat
     1861 gaagagacgg cgcagaaact gatcgatgcc gcactggaaa aaggcggacg ggataatatt
     1921 tcagttgcac tggcaactgt ttcacataat acggaggaat gagaacgatg gacaaggata
     1981 aactcatagg aacccgactg gatggccgct atgaactgac ggaactggtc ggcgaaggcg
     2041 gcatggcaga tgtttaccgt gcagtagatg tgctggataa ccgtgtggtc gctgtaaaga
     2101 tcctcaagca ggaatattcc gaaagcgaag agttccagcg ccggttccgg gacgaatcca
     2161 aggcgatcgc catgatgtcc catcccaata tcgtgaagat ctacgatatg ggcttttcag
     2221 acaagatgca gtatatcgtc atggagtaca ttgacggcat taccctgaaa gattacatcg
     2281 acagcgaaca cgtgctgaac tggaaggatg ccgtacactt tgtcatccag atcctccgtg
     2341 cgctccagca cgcccacagc cgtggcattg ttcaccggga catcaagccc cagaacatca
     2401 tgctgctgac agacggcacg atcaaggtca tggacttcgg tatcgccaag ttcgcccggg
     2461 aagagagccg gactgccaca gaccaggcaa tcggtacggt gcattatatc agcccggagc
     2521 aggctcgtgg cgatgtgacc gatgccaaga gcgacctcta ttccgtcggt gtcatgtttt
     2581 atgagatgct cactggcaga aagcctttcg acacggataa cccggtttct atcgctgtga
     2641 tgcatatgca gaatggtgcc gggcgcccca gtgatatcaa cccgaacatt ccctccggtc
     2701 tggaagagat catcatgcac gccatggaaa aggactccgc aaagcggtat cagacggcag
     2761 cggatatgat ccgggacatt gaggcattca aggcaaacaa ccagatcatc ttcggctatt
     2821 acaatgcgcc ggaacagacc cagtatttca caccgccgga agagaaccgg aaccggacgc
     2881 cccagcgtcg gaacggatac gattccggca gcagaccgga ctactatgaa aacaactacc
     2941 gggaggaaaa cgagccggag caggaacagt ccaagtcgct ggtcgttccg attctgactg
     3001 ccgtgaccat cgttgtggtc gttgtggcag caatcgtgat cttcctgctg gtgggacagg
     3061 gaacaaacgg ttccagcggc tctaccgatg aagtgccgaa ggtcattggc atgaactatg
     3121 acgatgcggt gctgaactat cccaacattg agtttgaagt ggcagagcag gaatacacag
     3181 ataaatatga agaaaacatc atctataagc agaacgttga ggctggtcag ttcgtgaaga
     3241 agaacaagga cggcaagatc gaactggcag tcgctgtcag caagggcatc cagaaggtgc
     3301 agatcccgga tctgaccaac tatacggcac aggaggcaga ggatgcgctg aaggcgctgg
     3361 gtctgaccgc agagcagaaa aagacccaga gtacctctga ggaaaacatt gaggcagacc
     3421 acgtcatccg taccgaaccg ggcaaggatg aagaggttgc cgtaggttcg aacgttgtga
     3481 tctatgtcag ccagggcatt gcagtcgatg agacagatgt accgaagttt atcggcatga
     3541 gccaggagga tgcaaagaaa gaggcggaca gccgaggact caagctggaa gtgacagaag
     3601 caccctctac agaggaaaag ggcattgtca cagagcagga tccggagccg ggcaaggtag
     3661 ttgtctccgg ttcgaccgtt aagctgaccg tcagcaacgg cgaagagccg gagggcgaag
     3721 tgacctatgg tctggaaaat ctgccggtct ctgagatgag cggtatgtat acgctgagct
     3781 ttaccacaga cgaggacggt gtgatcgcat cccgtcagtt tgtggcagag agtctgacag
     3841 acgcgtccgt cagcgtaaag gtcaagggca agggcagcaa gaccgtaaag gtctccatca
     3901 ccagcgccca gagcggcaag acagaagttc tcggcacgta tacgtttgat tttgccggca
     3961 agaaatttac ggcatccagc gaaaatattg caggagcgat tgaaggtgtc gctgtgccga
     4021 ctgtgccggt aacaacgact gttcctgcgg aaactgctgc gccggacaat gccggggagg
     4081 tagaataaag cgatgacaaa actccctgtt tccattccgg aaaatgcaca gggcagaatt
     4141 atcacatcgg tgggagttct ctatacggta gagacctctt ccggtatctg cacctgtaaa
     4201 gcaagaggca tattccgcaa gcgtggcatc acaccctatg tgggcgacta cgttatgctg
     4261 tccgaggacg gcgtgatctc agagatcctg ccccggaaaa acagcattct gcgtccgccg
     4321 gtgtcgaatc tggatcagat cttttttgtg acttctacct gtcacccgac cccgaacctg
     4381 ctgctgttgg attcgtttct cacggtggcg atataccagg atatccaacc ggctgtgatc
     4441 ctgacaaagg ttgaccttga caggggcgaa aagctggaat cgctgtacgc tgatgccggc
     4501 atcccggtgt atctggtgga ttatgacaag ccggagacca tcgacaatgt ggcaaagctg
     4561 ctggacggaa agatcagcat gatgaccggc aattccggtg tgggaaagtc tacgctgctc
     4621 aatgccattg ccccggaact ccagctcgcc accggagaga tcagcgaaaa gctgggacga
     4681 ggaaaacata ccacacgcca ggtgcagctt tatccctgcg ccggcggcta tctggcagac
     4741 acgccgggct tttctacctt tgaggtggaa cagtatcttc agatggacaa ggactgcatc
     4801 gccgactgtt tcccggagtt ccgaccttat ctggggcagt gccggttcag tgactgcgcc
     4861 catgtgtgcg aaaagggctg tgccgtggtg gaggcagtcc gggcaggaaa aattgccaag
     4921 agccgtcatg acgcttatgt ttctctgtac cagacggcaa aacagcgaaa ggcgtgggaa
     4981 tcatgaaaca ggcagtgatt tttgccggag cgtcggtgca accggagcaa cagcctcctg
     5041 tcccggcggc agacctctac gtctgtgcgg atgccggggt gaaactggcg caggcgcttg
     5101 gtgttgtgcc ggattggatc gtgggtgact ttgactccct gggcagtgtg ccggaggggg
     5161 aaaatgtgga accgttttcc cccatgaaag acgat
//
LOCUS       DAWENO010000146         5110 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6194_length_5110_cov_7.382196, whole genome shotgun sequence.
ACCESSION   DAWENO010000146 DAWENO010000000
VERSION     DAWENO010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6194_length_5110_cov_7.382196"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1629..1638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2455..2464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1067 a         1382 c         1270 g         1371 t
ORIGIN      
        1 gtgatccagg tcagccttat ccgaaatttt gctggtggcg tggcgtaaaa atgcagtcgg
       61 cacttcttcg gcggccatgc cgcatccatc gtccacgatt cgcatatagg ttgttccgcc
      121 atgctggatt tctacggtaa tgtgtttcgc accggcgtcg atggcgtttt ccacgacctc
      181 cttgatgacg gaggacgggc gctcaatcac ttcgccggcg gcgatcagtt cggaaatttc
      241 tttccgtaaa acggaaatat gcggcatgac aaacaccttc tttctaagcg gcatttcgcc
      301 gcaggttcgg acagcacagc gcattgtttt tgtgcggtgc tgtcacagtg ggattatgat
      361 aacattgcgg tgaggtctgc caggagttcc tggcactctg catcgctcag ttctgccaga
      421 tgtgtttttt gcagggctgc aatgacacgt tcctgctgca tggcggcaaa gctgatctgt
      481 ccgttctgtg cagtctgtct ggacattcct gctgcggctt gcttttccat ttccgccagt
      541 agttcctttg cacgctttac cacttgatcc ggcagtccgg caagctttgc aacagcaatg
      601 ccgtaactgt catcgacacc gccggggacg atcttccgca ggaaccggat ggaatccccg
      661 tgctttttca cggcaacgct gtaatttttg atgcccggaa tctcctgttc cagatccagc
      721 agttcgtggt aatgggtcgc aaatagcgtc ttacagccga tcttccggct gctgatgtat
      781 tctgccacag cacgggcaat gctgacaccg tccagcgtgg acgtgcctct gcccacctcg
      841 tccagaatga ccaggctgtt ttttgtggcg tagtttaaga tgtcggagac ttcccgcatt
      901 tccaccatga aagtactctg tccggcggtc aggtcgtccg atgcgccgat acgggtgaaa
      961 attttgtcta ccacagagat tttcgcatac tctgccggaa caaatgaacc gatctgtgcc
     1021 atcagtgtaa tcagtgcgac ctggcgcata taggtggact tacctgccat attcggaccg
     1081 gtaatcatca gcattcgctg cgccttggtg tccaggtatg tgtcattcgg aacaaaaact
     1141 tcttctgtga gcatccgctc tactaccggg tgtctgccgc cggtgatctg gatgctgccg
     1201 tctacggcaa tttccggctt ggaataatcg ttttccattg ccacatttgc aaaggatgcc
     1261 agtacgtcta cctgtgccac agcggcagcc gtttcctgta cctttttcag catggacgcc
     1321 aggcagtccc gaacttcgga gaaaatgccc tcttccaggc gcaggacacg gtcttttgca
     1381 ccgaggatct cgttttccac gtttttcagt tcttcggtga taaagcgctc actatttgcc
     1441 agggtctgtt tccggatata atcggacggg atcagatcat aataggaccg ggtgacttcc
     1501 aggtaatacc cgaatacccg gttatagccg atcttcaagc ctttgatgcc tgtccgttcc
     1561 cgttctctct ggagaatctc gtcaatgatc tgtgtgccgc cgttcataat gcggcgctgg
     1621 cgatccagnn nnnnnnnnat ccatcccgga tgacgccgcc gtccttcaag gtcataggcg
     1681 gatcgtctac gatggcattt tccaccagtg cagagacacg ttccaacggc gagatctgct
     1741 gttccagtgt gttcagcagc cgggaatccc ctgccagctt ttgcagctgt gccttcagtt
     1801 ccggcagctg ctgtgctgtc atggaaaggg atttcagatc acggggcgtt gccttttggt
     1861 agatcacacg tgtcataaga cgttccaggt catagacgtt gtccagcagg tcacgcactt
     1921 ccatgaggac aatgcttttc ttgacaaacg catctaccgc atccagacga gccatgatct
     1981 ttaccggctg caccagcggc tgttccagcc aggttttcag cagacgtttt cccatagcag
     2041 ttcttgcatc atccagcagc cagagcagtg aacctttccg ctctttgttg cggatggtct
     2101 ctgtcagttc cagattgcgg cgtgcgttga ggtccagtcc catggcggat gcactgtctg
     2161 ccacttcaat ggagacaaat cgggcaatgt tccgcttttg ggtctcccgg atgtagtcca
     2221 gcattccgca gacagcactc acatctgcgc cgtcctctgt catgcccagc agatccagag
     2281 aggaaacgcc gaactgctgg cagaccagtt cccggttctg taccgggtca aagcagatat
     2341 catcccgaag agaaactgcg cactgcagcc gaaccttaat gaagtccata acggatttca
     2401 gagaaagcgc ctggctgttc atgaccagct cagccggcat ataccgggac agcannnnnn
     2461 nnnnggacag cacatccatg atgcggacag acagatcgga tgcctcctga ggaatggtca
     2521 gcgacatttc gccggtggaa agatcggcaa agcagagcgc acaagagccg tcatttccat
     2581 agtacagcgt gcagatgtag ttgttggcat cgtcgtccag catactgctt tcgatcagcg
     2641 tacccggtgt gacgatccgg atcacatcac gctccaccag ccctttgctc agcgccgggt
     2701 ctgtcatctg ctcacagatt gcaattttat agcccttgtc caccaggcgt ttcagataga
     2761 gttctacgct gtggtacggc acgccgcaca tcggcgcacg ttcttccaga ccgcattcct
     2821 ttccggtcag tgtcagttcc aattctctgg acaccagaat ggcatcttca aagaacatct
     2881 catagaagtc tcccagccgg aaaaagagga tacagtcatt gtatttttct ttggttgctg
     2941 cgtactgctg catcattggc gacagctttt cttttggaat atctgctaaa ttcacgggat
     3001 tcacctccga atattatgaa aatcgagtta gtgacagccg ccgcaggagg cgcagttgcc
     3061 ggaacaattg gacagatccg gatggcattt gtccgggtct tcgccgttgg cgcagaggga
     3121 caggatggtg tccacttcct gcatcatagc gttcatcgcc tgctttgcac tgtcaaaggc
     3181 acgcatattc gggttttcca tgatgcgaag atacagcgcc tggatatcct gctctagttt
     3241 ttccagcttt tccggatttc gtttttcttc cagcttttcg gtttcgtgct gataggacat
     3301 ctttttcaga ttgaatgcct ggatgtcgtt ttgcagatcg gtatctgcat cgttatccgc
     3361 ttttgcacgc cagtatgcgg tatatctcgg gtcagcctgg atggcttttc ccaactgccg
     3421 tactgctgcc tctacttctg tcatttttgt tcctcctctg ccaattcgcc cagaactgcc
     3481 cagtttcttg cctctgtgat gcggacgttc tggaatgttc cgatcagcga cggatcgccg
     3541 ggaaattcta cgatgataaa tgcgttatct tttcccacga gccgtcctgg gcgcttgctt
     3601 tcgccgtcta ccagcacccg cagggttctg ccgataaaac gcttgttgtg ttccgatgcg
     3661 atttcacgct gcagggaaag caggcgttcc atacggtcac gcttttctgc atctgtagta
     3721 tggtcttcca tttctgccgc ttttgtgcca cgtcgcttgg aatagataaa cgtgtaaatg
     3781 ttgtcgtact gcacccgctg taccagatcc agcgttccct ggaaatcttc ctctgtttcg
     3841 tccgggaatc cgacgatcag atcggtcgtc aaagaaaaat ccgggtcacg ccggcgaaga
     3901 tagtttacgc tttccatata cttttctacg gtatattttc ggttcatgcg gcgcagtaca
     3961 tcgttgctgc cactctgtac cggcaggtgc agatggtgtc cgactttgct gcactgcaat
     4021 atggtgtcca gcagttccgg cgttgcatcc ttcgggtggg acgacataaa ccgaatcacg
     4081 aattcgcctg ggatatcgtt gattttttgc aggagctgtg caaatgtcag cggctgttcc
     4141 aggtctttcc cgtaggaatt cacattctgt cccagcagca tgatctcttt gtagccgtct
     4201 gccaccagct ggcgtacttc ccggaggatc tgttccgggt cacggcttcg ttctctgcca
     4261 cggacatacg gcacaataca gtatgtgcag aaattgttgc agccaaacat aataggaacg
     4321 gacgccttga acggatgttc ccgggacggg acgataccct catacatagc gtcgtattcg
     4381 ccggtgtcac aggcaaactt tttcccctgg aagtgttcca gcaggagtcc gggaaaacgg
     4441 cttagcgagg cggtgccgaa aacaatatcc acgtaaggat agtgcgaccg cacaaattca
     4501 gcggtatgct cctccgacgc catacatccg caaagcacca ggatcagatt ggggttggtc
     4561 tttttgtatt tcttgatatt gcccagcatt ccatagactc tgtcctcggc gctttcacgg
     4621 acggcacagg tgttgaacag gatcagatct gccttttcat aggagtctgt catggtatag
     4681 cccaactctg tcagcattcc acggatgcgc tctccgtcag aaacattcag ctgacagcca
     4741 aagctatgca cacacgcata gaccgtgtcc cgttctgcaa acagatacgg ctgcagccgg
     4801 gtgcgcattg cctccgttac tgctgccgca gtttcatttt tctgacgttc catagcaatc
     4861 catctccatt tctttccggt acagcgctgc tgccccaaaa aaggtcatta cactttatta
     4921 tagcataatt cacttttctt tgcaagaatt tttttcgaat tttctgtgga aaacaagggc
     4981 attgctgctt aaaatagatt cctatgggca ggaaaaggat tttcagataa tttccagcgg
     5041 cttttcacaa agctatcaca gatcactggt atgcgactcc cagcgtcagt tggagcaaag
     5101 gttgcatggg
//
LOCUS       DAWENO010000147         5086 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6218_length_5086_cov_9.086861, whole genome shotgun sequence.
ACCESSION   DAWENO010000147 DAWENO010000000
VERSION     DAWENO010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_5086_cov_9.086861"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3270..3279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1155 a         1445 c         1292 g         1184 t
ORIGIN      
        1 ggtacaatat catgaaaaca ctggaaggta tgtatgacgg caagggactg aaaattgcca
       61 ttgtggcatc ccgtttcaac gaattcatca ccaccaagct ggtaggcggc gctgaggact
      121 gcctgctgcg ccacggcaca gacggcgacg acatcaccct ggcatgggta ccgggcgcat
      181 ttgaaattcc gctggctgcc aaaaagctgg cattctccgg caagtatgac gccatcatct
      241 gcgtcggcgc agtcatccgt ggctctacga gccactatga ttatgtttgc gctgaggtct
      301 ccaagggcat tgcacaggtg ggtctggaat ccggtctgcc ggtactgttc ggcgtactga
      361 ccaccgacac catcgaacag gcgatcgagc gtgccggcac aaaggcaggc aacaagggct
      421 atgactgcgc tatgtctgca ctggaaatgg tcagcctgtt caagaacgcc tgaaaaacaa
      481 gacactttcc gcatggttga aacctgcaaa tctgaaattc ccgtaaagtg agtgatttta
      541 tgaaaaattt cattttcttt gacattgacg gcacgatcat ctccgaggac ggctatctgc
      601 cggaatccgc agtccgtgcc atccaggcag cccggaaaaa gggcaacgcc accctcatca
      661 acaccggacg caccgccatg aatgtagacc cgtttctccg ggagatcggc tttgacggct
      721 acatttacgg ctgcggcaca gaactggaat atgacggaaa ggtcctgttc cacgaaaagc
      781 agaccccggc acgcagccgt gaaatggtag aactggtgcg ccgcaccaac gttgccgttc
      841 tgtatgagcg gagcgacgcc atattcatcg accgcaaggc gagaacgctg ccgaacctgc
      901 aaaagctgct ggagctctac gaaagcaagc agctgccgtt cgccgatgtg ccggaggatg
      961 gggactggta cacggacaag tttgtcatct ggtatgacgg cgaaagcgat ttggagggtt
     1021 tccaggcagg tctgacggga aagtttgact atatcgaccg tggaaacggt tttgctgaaa
     1081 tggtgccctg cggctgttcc aaagccaccg gcatggaaaa gatgatgcag ctgctgggtg
     1141 cagaccgctc tcatgttttc gccgtgggcg acagtctgaa cgatcgtccc atgctggagg
     1201 ctgccggaac gggcatcgcc atgggcgaca gtcccatgct gcacccctat gcggactaca
     1261 tcaccgcaaa ccttgctgac gacggcttgg aaaaggcgct ggcacacttc gacttgatct
     1321 aaggttatat ccccaaaagc aaactcccgc agtttcagaa atccttgaaa ctgcgggagt
     1381 tttttatcgt atcacaatat tttcaaatca ttcagcgctt tcctgctttg cagcatcccg
     1441 atccatgcgg atggctttca gcacgatggc gcattccaga cgctttttct cgatctcaca
     1501 ggacagcttg tatgccttgt ggacatcgcc gctgtactgg tagttgttcg cattacagcc
     1561 gccgctgcag tagaatcttg cccagcagtt ccggcagtcc ggcttgctgt agatatgggt
     1621 cttggcgaag tcggacttca tttcacggtt gaatgtgccg tcgtccacgt tgcccatctt
     1681 ataatctgcc tcgccgacaa actggtgaca tgggtagata tcgccgtccg gtgtaattgc
     1741 cacatactcg ttgccgcagc cgcagccccg gagacgctta atggcgcagg gtccctgatc
     1801 caggtccagc atgaagtgga agaagttgaa cttcttgccg gttgcctcat agtccaggat
     1861 cttctgcatc agccgttcgt actcctcaaa gatacggggc aggtctgcct cggtgatggc
     1921 atagggttcc ttcgggtcag cgataaccgg ctctacagag atctggtcga aaccggcatc
     1981 cagcagtgca aagacgtcgt cagagaagtc aaggttatac ttggtgtatg tgccacggac
     2041 gtagtattcc ttttcaccac gctggtctac cagctttttg tacatgggga cgattctgtc
     2101 atacgaaccc ttgccgtcca cacggggacg catccggtcg ttgacatcct ttctgccgtc
     2161 gatggacaga acaacgttgg acatctcccg gttaatgaag tcgatcttgt catcggtgag
     2221 aagcataccg tttgtggtga tggtgaaccg gaacttcttg ttgtagaccg gttcctgaga
     2281 acgggcgtat tccacgattt tcttgacggt ctcgaagttc atcagcggtt cgccgccgaa
     2341 gaagtccatt tccagattca cacgatcccc ggaatttttc agcaggaaat ccactgcctt
     2401 tttgccggtc tcataggaca tcagctttct gccctcgccg aagtcgccgg tagaggcaaa
     2461 acagtactga cagcgcaggt tgcagtcgtg ggcgatgttc agacacatcg ccttgatagg
     2521 agatgccaca gcgaagttgg catatttttc gtagtcatcc ggggagaaga ggatgccctc
     2581 gtcatatgcc tccaggatct cggcatagca ggaacggatc tcttccgggt catagaaccg
     2641 ggaaagcttt gtcacgacct cttccggaca ttccggtgca aaaggcggtg cgatattgtc
     2701 cagcatatca taggtcagct cgtccaccac atgaacgccg ccgctgttta cgtccaatac
     2761 cacattatag tcgcccaatt tatacttatg aatcataatt ctgtttcctc tttccagctg
     2821 catggggaaa agcagcataa ctcttataaa tgcgaaaaaa gggacaacga actgtccctt
     2881 ttttctatcg gttgcagact tgcgttaagc ggttcaaaaa ccacttagtg ttcgcacttc
     2941 tggttcgcta ctgtgcagga agtcttgcaa gcagactgac aggaagcctg acacttgccg
     3001 cagccgccct ttgcagcaga agccttcagg tttgctgcat tgatggtctt gatgtgcttc
     3061 attcgaaacc ctcccccaaa aaaagtttaa ctacattata tcacaaaatc accggatttt
     3121 caagcgttcc atgcaaaata ggaaaaaagt ttacatcttg caaaagaacc ctccggctat
     3181 ctgggcggtt tccgatgggg tgcattgacg ccggaaatcc caccccagac accgccgccg
     3241 cagagacaca acagccgcac cggactgccn nnnnnnnnna gaccaggctc gccagcgtca
     3301 gcactgccca caggaagagt ccgcagaaca gtccgcagcg ccagcctttc cggcggcggc
     3361 ggaatccggc aaagcgaccc atgccgtacc cggaaaacac cagcgctata cctgccataa
     3421 gacgcagcat ccactccggc gcatcggcga aaagcatcat cgccgccagg atgctcagta
     3481 cgccggtcag aatccccagc cccaccggca gcgccgccag caaataccag ccgccggata
     3541 cccaaaggct tttccgggac tggggtcgtg tggtttccat gggctgcacc tccgttaaac
     3601 actgttatcc cagcatatgc agcacccgcc agtgatagaa cgcaaaaacg gactgtacga
     3661 atacagcccg ttcttttgcg aaaatgcact gggattctta gtccttcagc ataccctggt
     3721 cggagtcgtc agaacccttc tttttcttct tggatgcttt cttttccttg gcagcagcga
     3781 tctgtgcctc ctgcttgcgc tttgcattct cggtcatggt cagattttcc tgtactgccc
     3841 agttcttgat gcgcagcttg ttgcggttgc tggttgtctc aatgacgata ttatcctcat
     3901 tgacctgtac caccagacct acgatgccgc cgatggtaac gatctcgtca ccgacctgga
     3961 ggctgtcctg cattgccttt gcttccttgt cctttttctt ctgcggacga atcagaatga
     4021 aatacatcac tgcgaacagc agcaccataa agatcagata gcccactgtg ccaaagccgc
     4081 cgacggatgt actcgtagaa gatgtgccgc tggcaaatgc agtcatgctg caaacagcgc
     4141 ctgtcagagc tgctgtgctg gcagccagtc ccagtcttgc gaaattctta ctcatgttca
     4201 aaagcccttt ctacgtttgc cctgcgcttt cgggaaaaat gcttacgcct tgctgcgcag
     4261 cacttcgtcg atggacgcag cggctgtttt tccggcaccc atcgccagaa tgacggttgc
     4321 cgcaccggtg acagcatcgc cgccggcata aaccttgtcc tttgtggtag ccatttcctc
     4381 gttgactacg agacagcccc accgatttgt gtccaagccc tctgttgtcg aagagatcag
     4441 cggattcggc gaagtaccga tcgccatgat aaccgtatcc acatccagga caaactcgct
     4501 gtccggaaca gcaaccggtc tgcgtctgcc gctttcgtcc ggttcgccca gttccatgcg
     4561 gatgcacttc aagccgttga ctcttccgtt ttcatcgccc agaatctcta ccggattgca
     4621 caggttctgg aactctacgc cctcttcctt ggcgtggtgt acctcttcca gacgtgccgg
     4681 catctctgcc tcagaacgac ggtatacgat gtacacgtgt tctgcgccca tacgcagcgc
     4741 acttctggca gcatccattg ccacgttacc gccgccgaca acagccacaa ccttcgactg
     4801 gatgaccggt gtcgcatact tcgggtcata gccgtgcatc aggttgatac gggtcagata
     4861 ctcatttgcg gagcatacgc ccaccagacc ttcgcccgga atgttcatga accggggcag
     4921 acctgcaccg gaaccgataa agactgcatc atagccgcct gccatcagtt cgtcgatgga
     4981 caggatcttg ccgatgacca tatccggctg gatatctacg ccggcatcct tcagcgactg
     5041 gatctcctgc tgtacaatgg ttttcgggag acggaactcc ggaata
//
LOCUS       DAWENO010000148         5057 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6253_length_5057_cov_14.028389, whole genome shotgun sequence.
ACCESSION   DAWENO010000148 DAWENO010000000
VERSION     DAWENO010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_5057_cov_14.028389"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1155 a         1458 c         1472 g          972 t
ORIGIN      
        1 ccccagcccg gtgggtgaga cttccgttca cgggctggat ctggactggc ggcggcgtgt
       61 gatctcacct atgccatccg caccgctgtg ccggacggcg tcagtctgac ctggcggcaa
      121 agcgacacgg aaaagctgat cttcgtgcag aacttcggag acagcgcagc cgcagtgcag
      181 ctggatcagc cctatgaaaa cgtcctcacc ggagagactg tctccggcag tctgaatata
      241 gaaaaatacg gctttgcggt attggcaaag cagatataga tttttgattt ataggaggta
      301 agcacagaaa tgaacattcc cgaattactg cagcagctga cgctggaaga aaaagccggt
      361 ctctgttccg gcggcgactt ctggcacacc aaggcggtgg agcgcctggg catccctgcc
      421 atgatgatga gcgacggccc tcacggactg cgcaagcagg atgaacaggg cgaccacatg
      481 ggcgtaaacg acagcatcaa agctgtatgc tttcctgccg gctgcgccac agcctcttcc
      541 tttgaccggg aactggtgga acgcatgggc aaggcgctgg gcaacgagtg ccaggcggaa
      601 aatgtgggcg tgctgctggg accggcggtc aacatcaagc gttctccgct ctgcggcaga
      661 aacttcgagt actactccga agacccttat gttgccggcg aaatggcgac ctatttcatc
      721 cgtggcgtac agagccagga tgtgggaacc agcatcaaac acttttatgc caacaaccag
      781 gaacaccgcc gcatgagcag ttcttcggag atgagcgagc gcaccgcacg ggagatctat
      841 ctggcggcgt ttgagcgtcc catccgcaag gcaaagccct ggacggtgat gtgcgcctat
      901 aaccggatca acggcgtcta tgccgctgag aacaaagagg cgctcacgga tattctccgg
      961 ggcgagtgga atttccacgg ctatgtggtc agcgactggg gcgcagtcaa cgaccgtgtg
     1021 gcagacctgg aggcaggcat ggatctggaa atgccctctt cctgcggtat gcgtgatgca
     1081 gagatcgtca aggcagtgcg ctgcggcaga ctgccggaat ctgtcctgga cagagccgtc
     1141 acacgcatcc tgcgcatcgt ccagcgctat ctggacggca gacggccggg cgctgtgttc
     1201 gaccgggacg cagaccacga actggcacgg gagatcgcat cccagtgcat ggtgctgctg
     1261 aaaaatgaag atcacatcct gccgctggac aagacggttc cggcagcgtt tatcggaaaa
     1321 tttgcgcagt cgccccggtt ccagggcggc ggcagctctc acatcaactg ccacaaggtg
     1381 gaatctgcgc tggaaatggc aaaggattat ccggtcacct atgcccaggg ctatgagacc
     1441 tcggacgatg tgatccggga cgatctgatc gcagaggctg tggcagcggc aaaggcggcg
     1501 aaggtggctg tggtatttgc cgggctgccg gacatctttg aatccgaggg ctatgaccgg
     1561 gagcatatgc gtctgccgga ctgtcagaat gcgttgatcg aggcagttgc cgcagcgaat
     1621 cccaacacag tcgttgttct ccacggcggt tctccggtgg agatgccctg gctgggaaag
     1681 gtcaagggcg tgctggaggc atacctctgc ggtcaggctg tgggcggcgc aacggtgcag
     1741 atcctgtacg gcgacgtgaa ccccagcggt cacctggcag agacgttccc ggtcaagctg
     1801 gaggacaacc cgtcctatgt ggcataccag ggcgagggcg atgtgacgga ataccgggaa
     1861 ggcgttttcg tgggctaccg ctattacgcc accaaaaaga tgccggtgct gttccctttc
     1921 ggctatgggc tgagctatac cacctttgca atctccaacc tgcgcaccga acaggacgag
     1981 tatcagtctc acgacgtgat gacggtttcg gtagacgtga ccaacaccgg cgaccgggca
     2041 ggcaaggaag tggtgcagct gtatgtgctg ccaaagacca aaaccgactc ggtacagcgt
     2101 ccggtgcagg aactgcgtgg ctttgaaaag gtggaactcc agccgggcga gaccaaaacc
     2161 gtcaccttta ccctgcacgg ttccagcgcc tttgcgtact actgcaccca gaaaaagcgc
     2221 tgggtcgtag agaccggcgc atatcagctg gctgccggca actcttcgga caacctcacc
     2281 gcagaaaaga ccgtcctcat ccacggagaa cgggcgctgc ctgcggagat cactttgaat
     2341 accaccatcg gcgacatcct gcgcatcccc ggcgcagacc aggagatcat gaaatatgcc
     2401 ggggcatttg gcatgaacga ttcctccgaa tcggactcca tgggcgaaag caccgcagat
     2461 atgatggaag ccatgatgcg atatatgccg gtccgtggcc tgctctcctt tggcggcgga
     2521 acggtctcca ttgcggaatg caaggcactt gtggaaaagc tgaacaccct tttggaacag
     2581 gcgaacctgc acaacaggga ctgaaatcaa tttctaatag agttcccctt gaaaaggcgt
     2641 ctccttgtgc tgggaggtaa cccctcagtc aagcctacgg cttgccagct cccctgttag
     2701 gggagcctat accaagttac ttcgttatct cataagaaat attccttttc aaagaaggca
     2761 atattataaa aattgatctt catagactga tgtgacaact tctccacctt tcaattgtca
     2821 gacggcgcat atcggggcaa cgaccggtat gcgccgtctg ctgtccgctc cgccgtcatc
     2881 tgcacccgga gattacagaa caccgtcacc gacagcaccg cctgaggcaa cacccagggg
     2941 atggtcacca cgggcagcat ctgcaggcca taccagccca gcagtgccag cagttccttt
     3001 ctgccgccgt gcatggcacg cattgccgca aggggtactt tccacgccgg cagttccgga
     3061 cgggcaaagc agacgaacgg cacgcaccac agccccaggc agacatagca ccaccccaga
     3121 aggcaaagtg tccccagcac cagcagctgc acggcggcga acagatacgg tgcgctttcc
     3181 gtatgggctg ccccggcctt tcccagctgg tatgcgccga acagacagag cgtcacggtc
     3241 tggagcagca gggtacgcag cagggtgttt tgcagcgcca gccacagctg ttttttccgc
     3301 ccggcaggac ggcggtgcgg cagcatggat accacggcgg tacaacgccg ccacagcgac
     3361 cagctgcacc agttccagac gagccacgcc ggaaccagcc agaaaagctg caccagccgc
     3421 cacagcagcg cctgctctgt caaaagggaa acaggcagtg tgttccggat gcgcagcacc
     3481 agcgccgccg caagcagttc cagcagccag agcaaactgc ccagcagcaa ccatccccag
     3541 caagccagtc caagccggcg gtgggcgtgg agcatttgtg cgcaaaattg ctggttctga
     3601 cgtatctgca tggcattccc ctccgatctc ttctgctcct atctttccct gcgctgcatg
     3661 attttatacg aaaggacgtg ttttttctgg cagaactgca ttttcgaatg gactgtcccg
     3721 accctatgac cctggaacaa tttctccggc ggcacaaggg cgtttcgcac cggatgctgg
     3781 tacggctcaa gcggatcccc ggcggcatct gttgcaacgg agcgcccatc cgcaccattg
     3841 atccggtacg atacggagac gagatcgttc tcaccgaccg ggaaaacgcc tcggcacttc
     3901 agccggcggc ggtttctgta ccggtgctgg acgagacgga cagctacatc atttacaaca
     3961 agccggcgca tatgccggtg catccctcgc aggggcatca cagcgacacc ctggggaatg
     4021 tgtttgccgc acagttcccg gagctgccgt ttcgcccggt gtaccggctg gaccgggaca
     4081 ccagcggcat ttgtctggct gccaagtctg cctacgccgc aaagcagctc cagggcagca
     4141 cccggaaaac ctatctggcg ctggtctgcg gcatcctcac cgaaagcggc acggtcgatg
     4201 cgcccatcgg caggcaggac ggctctgtca tccggcgttg tgtccgggca gacggaaagc
     4261 cggcagtcac ccactttgta ccgctccggc acagtgaccg gtataccctg ctttcgctcc
     4321 ggctggaaac gggaaggacg catcagatac gggtgcatat ggcgcatctg gggtatccgc
     4381 tcgccgggga tgacctgtac ggcggcgata ccagggattt ttcccatcat atgctgcatt
     4441 gtgattcgct ggagtttttg gatccggtga cgggagagtt gcggcagtat gcggcagaat
     4501 gcccatggga actgcaataa cactgccagt cgtgtgatac attgccgaac agaacaaggg
     4561 ttggcgagcg gcgagtcgcc gctgaccttt cgccccaatt taacgttcaa aaaagggcag
     4621 tgtcacggca gacaagctgc ctatgacccc tccggggaat ttatgtcata aataatctaa
     4681 tgtaacacat tgccagtcgt gtgatacact gccgaacaag gcaagggtag gcgataagct
     4741 tttctttttt tgcttctttt tttcttttca gtaaagaaaa aaagaaggga aattttatgt
     4801 aaatccctct gctgacatct cagcacaagg cacaccctgc gggcgccttt tcaagggaga
     4861 ctgaatactc ctctgccctg tcgggcatct cctctatgag aggagacaat ttggtggcaa
     4921 acgccataat aataacgtca accgcctgca caagagacat ccagaaaacc tacgctacac
     4981 gtacaggaaa aggcagacaa gggcgtttgc aagccccagg cggacagcga ggaacgagcg
     5041 attccgccgg acgggtt
//
LOCUS       DAWENO010000149         5031 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6282_length_5031_cov_8.981913, whole genome shotgun sequence.
ACCESSION   DAWENO010000149 DAWENO010000000
VERSION     DAWENO010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6282_length_5031_cov_8.981913"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2991..3000
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1100 a         1424 c         1191 g         1306 t
ORIGIN      
        1 gtatccagaa tttccggcgg cacaaaatgg ctgatgtcac cgccaaatgc ggcgatctgc
       61 tttaccacgc tggaactcag atacatattc tccgaagtcg ttgtcagaaa aacagtttct
      121 gcgccggcgt aaagctttcg gtttgccagt gccatctgaa attcatactc aaagtcactg
      181 actgcccgga gccccttgac aatggcggtt gcgccggcat ctttcacata gtctgccagc
      241 aggccctcgg aaatatcgac cgtcacattg ggtagatcct ttgtgacgca caagatcata
      301 tcttttcgct cctgcggaga aaagcaggcg ttatttttca taatgttttt ggagaccagc
      361 acgatcacct ggtcaaacag ctttgaggca cgggtgataa tgtccagatg tcctactgta
      421 accgggtcaa agctgccggg gcagactgcg attctcttca tctcttacac cgcatccttt
      481 ctgcacacgt atgttgtcac ggtgatcgca ccatacttct tctgcttttt ctgttccagc
      541 acgcctaccg tttccggcat ttccaagccg gtctcatgct cgcaaatcac gatgccctcg
      601 tctgccatgc gttccgccag cagcggcagc agcttttcgt gaaagccttt ccgatacggc
      661 ggatccagaa tcgcaatatc atactgcatg gcagtggttt gcagaaaaga ctgcgcctct
      721 gcctggtgca gctgcgcctg tgccgccagc tgtacggatt ccagattctc tttggtcatc
      781 tggagcgaac gtgcagaacg atccacaaaa actgccgtct ctgcgccacg gcttagtgcc
      841 tcaatgccca tctgtccact gcctgcaaac agatccagaa caacagcacc cggcagatcg
      901 aattggatcg cagaaaagat cgcctctttg actttatccg tcgtaggacg cacatccaga
      961 ccctctaatg ttttcagttt tctgcctttg gcactgccgg caatcacacg catacccatt
     1021 ctccttttgc attgtctccg caattgattt gatactttct tattatacaa cgaattatgt
     1081 caaatgtcca gctttttttc acaagcagag aattctctat tctcctcctg ccatctcctg
     1141 gatcaccgca tacagtgcct ctgccgtcgg acggctgatg ccggcagtgg attccagttc
     1201 ttccagggtc gcctgtttca gattcgccag ggtcttgaac cggagcagca gtttttgcgc
     1261 ctttttttca ccgatgcctc gcaccttggt cagttccatc tgataggagg atttggcatg
     1321 gatgctgtgc atatatgcca cagaaaagcg atgaacttca tcctgtatct gggtgagcag
     1381 atgaaatgct gaacgattcg ccgccagcga gatttcaccg ccgccggtgg aaatggcacg
     1441 ggtgcggtgt ttctggtctt ttaccatgcc aaacagcgga atgtgcagcc cgaattgctg
     1501 aatcaccgga gcgatggtgt tgacatgacc ttgaccgccg tccagcagga taagatccgg
     1561 caaacgggaa aagccaggat catcctgtgc tgcataatgc cgcagccggc gggtcagtgc
     1621 ctctttcatg caggcatagt cgtcctgtgt ggtatgctct ttcatgcgaa accgcttata
     1681 tgctttcttc tgcggtctgc cgttttcaaa cacgatcatg ccgcagacca tagaggtgga
     1741 ggacaggttg gagatatcat acgcctcaat atactgcggc ggctggggaa gtcccaggac
     1801 actgcccagt tcctccagcg cctgcacttc cctgcccgtt cggttaaagc gaattgccag
     1861 ttcttcgtta gcgttgttcc gggagagttg cacaaactgg cagagcgttc cacgctgcgg
     1921 cacagtgatg cgcacccgga atccggcaaa gccggtaaac agctgttcgt acatttcctg
     1981 gttcggcatc gcaaattcca gcacaatgtt tttggggata tctgcccggt gatgctcata
     2041 atactgtgtc agataggagt ccatgacttc ctcccgtgac gcattttcct ggaaaaagaa
     2101 agtctctttg tcgaacagcc gccgtccccg ataacgcaaa atagaaacgc actgcgttcc
     2161 gccgtgttct gccattgcga tcacgtctgc ctcttccagg gctgcgtcca taatcttctg
     2221 cgtctccccg gcttttgtta ccgcagcgat cctgtcccga agcatggcag cccgctcaaa
     2281 atccagttct tcagcggcac gctccatctc tgcctgcatc cgcttgacag aatcggtgct
     2341 gcccatctgg atatacgctt tcgcctgctg gatgatctcg gcatatgact cctgcgaaat
     2401 atagccacgg cagacaccca tgcactgctt gatatgataa ttcagacagg gacgggtttt
     2461 cttgaaatcc tccggaaagc gcctgtgaca ggtgggcaga cggaacacac gattcgtttc
     2521 ccgtgccgcc tcccgtgcca cgtaaccgct catatagggt cccaggtagt caccggattt
     2581 cttcttgttt ttttccaccg tcagtctggg atacggctca tcggaaaagc agagataggt
     2641 gtagcccttg tcatccttca gaaggatgtt gtacttcggt tggtgctgtt tgatgagact
     2701 gcactccagc acaagggctt cgtattccga gtcggtgaca ataaaatcgt agtcatacac
     2761 gtgttcgacc atactggcga ccttcggcgt atggtcggca gactcccgaa aataacttgt
     2821 cacacgattt ttcagattct ttgcttttcc cacgtagatg atctcagact tctgattttt
     2881 catcaaataa acacccggag atgttgtgag ctttgctgtt ttctccctta atgcaggtaa
     2941 acgacaattc ataacaacgc ctccgggtgt tggaatttgc ttgcagcgct nnnnnnnnnn
     3001 tcttatatta cgacagggct ttcagcgcac gctgtccgat gtcctttcga tagtgggcat
     3061 cctggaacga aatggttttc acgccggcat aagcagtttc cagtgcctcc ggcagtgtct
     3121 ctgctgttgc agtgacaccc agcacacgac cgcctgctgt cagaaatgtc tcgtcgtcca
     3181 gctttgtgcc tgcgtggtaa acaaagcagt ttacagtctg tccgttttca tccagaccgg
     3241 aaatcggcag accagtcaca tacttcttcg gataaccgcc gctcgccata accacgcaag
     3301 ccgcacagcc attcttccag cggatgtcca gctgatccag ggtaccgttc cagatcgcca
     3361 gcataatgtc taccagatcg gtatccagca gcggcagaac gacctgggtc tccgggtcgc
     3421 cgaaacggca gttatattcg atcaccttcg gtcccttggg cgtcagcatc aagccaaagt
     3481 acagacaacc ggtaaacggt ctgccctctt cctgcatggc acgaatggtc ggcaggaaaa
     3541 tagtatccat acactgctgt gcaatatctg cagtatagta cgggttcggt gcaactgtac
     3601 ccatgccgcc ggtattcaga ccctggtcac cgtccagcgc acgcttatga tccatggaag
     3661 agaccatcgg ccttaccgtc ttaccatcgg taaagctgag gacggaaacc tccggaccgg
     3721 tcagaaattc ttcaatgacg atctgagaac cgctctctcc gaagatcttg tcctccatga
     3781 tagaggcgat ggcatcgaat gcttccttct cattctgtgc aatgacaacg cccttgccca
     3841 gcgccagacc atccgccttg atgacagtcg gatactcgtt cttgtctttc agataagcct
     3901 cagctgcttt cggatcatcg aatacacgat attctgcggt cggaatgtga tacttctgca
     3961 tcagttcctt ggaaaacacc ttgctgcctt ccagaattgc cgcatttttc ttgggaccaa
     4021 aggtcataaa gccctctgcc tgcatggcat ctgccatgcc cagaaccagc ggatcatccg
     4081 gtgcaacaaa gaccagatct acttccagct cttttgccag cttgaccatg ccgtcaatat
     4141 cggttgcctt taccggaaag cacttggcat acttggcaat accgccgttg ccgggtgcgc
     4201 agtacagcgc atctaccttc ggactctctg ccagtttcat gacgatggcg tgttctcttc
     4261 cgccgccgcc gacaactaaa atcttcatgc tgtctttcct cccggatcag tgatggaaca
     4321 gacggatgcc tgtgaatgcc agtgccatgt tgtacttatt gcaggttgca atcacatggt
     4381 catcccggat agaaccgccc ggctctgcga tatactgcac gccgctcttg tgcgcacgct
     4441 cgatgttatc gccgaacggg aagaatgcgt cagaacccag gcaaacttct gtgttctgtg
     4501 ccagccattc cttctgctct tccttggtga acacttccgg ctttacctta aaagtgttct
     4561 gccatacgcc gtctgccagc acatccatgt actcatcaga aatgtacaca tcaatggcat
     4621 tgtcacgatc cggacggcgg atgccatcca cgaactgcag tcccataacc ttgggatgct
     4681 gacgcagcca ccagatatct gccttattgc cggcaagacg tgtgcagtgg atacgggact
     4741 gctgtcctgc gccaataccg attgcctgac catccttgac atagcatacg gagttggact
     4801 gggtatattt cagcgtaatc agagagatca gcaggtctct cttatacttt tccggaatct
     4861 ccttgttgtc ggtgactaca ttggacagca tctcttcgtc gatcttgagg aagttgtgac
     4921 cctgttcaaa tgtaacaccg aacacctgtt tccgctccag gtctgccgga cggtatgccg
     4981 ggtcgatctg gatgatgttg taattgccct ttttcttgcc ctttaagatc t
//
LOCUS       DAWENO010000150         5029 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6285_length_5029_cov_11.091074, whole genome shotgun sequence.
ACCESSION   DAWENO010000150 DAWENO010000000
VERSION     DAWENO010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_5029_cov_11.091074"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    646..655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1054 a         1471 c         1325 g         1169 t
ORIGIN      
        1 gccccgattc ttccaaaaac ttgacaaaat cccgggaatc cggtataatt agtgtcatat
       61 caaaacgtga gcagagagga gttcaacagt tctatgatcg gagttgcact ggaaggcggc
      121 gctgaacgca gttcttttac cgccggcgta atggatgcgc tgatggcaca tgatttctat
      181 gccggtgcag catccggaac atccgccgga gcaggctgtg tgttaaacta ccgttccggg
      241 cagcagggcg tcgctctgga aatgatgatt atggaacgcc acagccgtta tttcggcatc
      301 cgccacatgg cgcatactgg acggtttctc aacctggatc acatggcaca cgagtacgcc
      361 agccgcatca gctgggacgc ataccggcat ccgtcgatgc agaccgattt tgtcgccacc
      421 tgctgcgagg acggcaagcc ggcgtacctc accgatgcag gcagcaagca gcgcctgttt
      481 acagcaatca aggcatcctg cgccctgccc attatctgtg aaccggtaga gctggacggc
      541 agacactatg tagacggcag catcgttggc ccgatcccgt tcccgcagat gcaggaacgg
      601 gatcgggtca gcgatgctgg cgtctacaga gtggctgccg tcccgnnnnn nnnnncgttg
      661 acccgatccc gttcctgcat ctgctggaac agggctgcga caaggtgctg gtcgttctca
      721 ccggcaacga gaatgcccat cccacagact acacccggct gcgtctcctg ctccggcggc
      781 tgtactacaa gaaatatccc atgctgtatc atgcgatcct cacacgcatc gcctgttatg
      841 agcgccaggt tcaggcaatg gaacaggcac agaaagagaa aaaggttctg gttctgcggc
      901 cgcagatcaa gccgatcccg ctgttcaccc agaacgagga aaaaattcgg gcgtattacc
      961 gccacggcta tgcgctcacg gaacagcgct gggaagagat cgccgctttt ctgggagaag
     1021 aggcacacac agcaacatga tcgcctgttt tgcggcaggc acggaaatag gaaaccaaaa
     1081 gcggtatgcg tttgtaagcg cataccgctt ttccgttttt ggggctagta ttcgattcct
     1141 tttcgtgcgg agatgccctt ttcataggga tgtttccgga gcgtcatttc cgtgacatag
     1201 tccgcacgtt ccagcagcgc cgcactgggg tcacgcccgg taatcacaac ctccagcgcc
     1261 tccggacggt tttccagaaa atccagcacc agctgttccg gcaggaatcc gcaggtaatg
     1321 caggagagca gttcgtccag caccagcatt gcatacgccc cgtgctgcac cgtctctgca
     1381 attttccgga actgcgccgt gtaccattct gccgccgcct gcttttccgc atccgtcatg
     1441 cggaacgaaa actttgcgct gggataggtc tgccacaatg tgacctgggg cagctgttcc
     1501 agaatgcgcc tttcgccgga attgtcctgt ttcatcagct gtgccaccag aacccgtttc
     1561 tgccacccgg cacagcgcac cgccagcccc attgccgccg tggtctttcc cttgccgtca
     1621 ccgcagtaaa tatgtaccag tccgctcatt tcgttttagg ctcctcatcc ggcaggagta
     1681 cttcccggtt tgtgccgtag aagatatcgc ctctgcctgc cagcatctca cagaaacgtt
     1741 ccagcctgtc ccagttctgc tgtccttcga actcatagct gtgtccccag atgtagaaga
     1801 tctgcggcgt ttccggctcg gattccagga actgtttcgc caggtcgaac agctgcggat
     1861 catcatggtg acaggtcggc tgaaaccgca tcagatctgc ctgctctgca aaggaatggg
     1921 agctttccac tccacgggca tagcgaatgc ccagacggtt cagttcggcg attacagtat
     1981 cgttgtacac gccgtaggga tatgccatgc ccaccggcgc tgtgccgtag agtttcgtga
     2041 tcgtctccgc attttcctgt aactcggcgg tcagttccgc cggagacagc tccgtcagat
     2101 tatagtgata tacgccgtgt actgccatct catgaccggc atagagttcc gggcattccg
     2161 ccaggttcag ccgatgcact tccagcacat cccggtacac ccaggtgccg tggtcaaagt
     2221 ccagcccggt gttcacgttg aacgtgcatt tcataccata gcggttcaag atgtccagca
     2281 gttttttgtc ctgtacattg ccgtcgtcat agctgaatgt caaggctttt tgatattgct
     2341 ttttccagtt catttgcgtt ctccttttcg gcagcataag ttgttgttgc cattatagca
     2401 cctgccgcag gatttgtcaa tgcgcagaaa aaatcgaaac ccttcgagtt tccacgaaca
     2461 tattgcccgg tctctccgaa tatgctatga tgaaagtctg tgaatctatc ctgaaaggag
     2521 tgctgccttc ttgcagccca cgtggaaact aaaacttgcc gctgccgcac tgacctgcgc
     2581 cgccttgcag cagccccagc cggttgtcca gccgctgccg ctgaaattct cccggtattt
     2641 tcccaaagca aaagaaaacc gtcctaaggg tgcatcccca ggacggtttt ctgcgcaact
     2701 gaaaatctat ggaatccgca ccaaagatgc ggcatccagc taatataagt tatcggtaaa
     2761 gcggaccata gtcagcgcag gcacgatagt ctgctgcctc cagatccttt gtgccgtagg
     2821 cactccaggt gtgcggacga tagatgcgtt cttccggcac attgtgcatg gaaaccggaa
     2881 tccgcagcat acttgccagc gtgataaggt ctgcgccgat gtgtccgtat gccaggcagc
     2941 cgtggtttgc accccagttt gccataacat ggtacacatc tgcaaatgcg ccctcaccgg
     3001 tcagattcgg cacaaaccag gtggtcggcc aggtcttgtc ggtgcgctcg tccagcggtt
     3061 tatggatctc atccggcagg tcaaccgtat agccctctgc gatctgcaag agcggctgtg
     3121 tgccgttgag gatattcaga cggatcatgg tcaccggcat ctctgcacgg gtgcggtagt
     3181 gagaagaaaa gccgccgcca cggaaatagc ccaggtttgc cggcgcccag tctgtggcat
     3241 ccagcatggc acggatatcc ggattcgtca catcccacca gggtttcatg acgccgtttc
     3301 cgtctgcgtc cttgcaggca cctgtgccgt ccagtgcagc cgcaccgctg ttgatgagat
     3361 ggataaagcc gttcgccgct cttccggacg gtgtaacgcc agtcacacgc ttgactgcct
     3421 ccgggctcca ataggtgcgg acatcggcaa agatggtcgc tgtgccggtc agcagcttgc
     3481 cgaacagcat tgccgtaccg ttcagaccgt cattctctgt agcgagaata gtaggctctt
     3541 tcttcccgtt ccagtcaaag ctggagttga ggatcgcctc ggtaaagtct ccgttcggca
     3601 gccagtctgt ccacatccgc tgtccctgga atccgcccag gatcgcattt ctgccgtggc
     3661 tctcttcttc ccagcccatt tccagcagct tgggattgcc gaagaaaata tcctgcacga
     3721 tcagcgtaaa cttgacgatg aattcccatt ccttcgcctt ttcctccggt gtccggggct
     3781 ttttgatgcc gaacgggtcg atctcccgat cttccggaac gtccttgttt acgtcatagc
     3841 cctccggaca gttttccttg acccatgcca gagccttttc gtactcgtca tggtcgtaga
     3901 tgcccagatg gatgcggcgc aggatctctg tcagatccac ccactctgca cgaatgccca
     3961 ggtatttctg gaaaaagtca gagttacagt aagaaccggc aatgcccatg gagacaccgc
     4021 ccagtcccac atatgcctta ttccgcatta cacctacggc aacagcgcac ttggcaaagc
     4081 gcaggatctt ttcctgtacg tctgccggaa cggtctggtc gtccttgtcc tggacatcgt
     4141 gaccgtagat agaaaacgcc ggcagtccac gctgtgcgtg cgccgccatg caggcagcca
     4201 gataaactgc gcccgggcgc tctgtgccgt taaagcccca cactgccttg atggtcagcg
     4261 ggttcagatc catggtctcg ctgccgtagc accagcaggg tgttacagaa agggttgcgc
     4321 agacattctg ggtctggaaa aaggtttcac attcatatgc ctctgcgccg ccgccgatgg
     4381 tggtaggagc gatgacacac tctaccttgg tgccgtcggg atagcgcagg ttcgattcga
     4441 tcagattctt ggcagcgtat gccatgttca tggtctgttc ttccagatct tcccgcacac
     4501 caaactgccg tccgtcaatg acaggacgga taccgattct aggatacatg atattccctc
     4561 atttctttga agtttttttg gaatatatag acgccgggta tgttcccggg tgataatttc
     4621 tcatggtttc agatgcggca atacaacgcc gtgccgcatc cggtgtttca attgcattca
     4681 ggttcagcag ctggatggct gcgtttccgt agactgtcgc ctcaacaggg ccggcagtca
     4741 cctgccgctg gcagaaatcc gcagtgagct ggcagagaaa gccgtctttc acgccgccac
     4801 ccagcatatg gatcaccgga taatgcgccc cggtgcaagc ctccagatct tccagcgccc
     4861 ggctgtattt cagcgccagg ctctcataaa tgcagcggaa aacggcaccc ggcgtttcag
     4921 ggatatgctg tccggttttg cggcagtacg cctgcacacg ctgcggaata tttccggacg
     4981 gtgtgaaaac cgggtcgtcc gggtcgatat aacaggacgg ctcgtggca
//
LOCUS       DAWENO010000151         4975 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6347_length_4975_cov_10.374187, whole genome shotgun sequence.
ACCESSION   DAWENO010000151 DAWENO010000000
VERSION     DAWENO010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6347_length_4975_cov_10.374187"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3034..3043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1097 a         1434 c         1282 g         1152 t
ORIGIN      
        1 gttcatcgaa cagacgggtt accagttcgg ttacagtctg gattcgctcg ccgctgttca
       61 gactcaatcc ctgcataatg acgttggaaa cgtgacgtgg cacattggga ttcagcacag
      121 acggtgccac caggttgtca ttttccatac gactaatggc agacggcggc atagagccag
      181 tcagaacacg atagatcaca gcgcaaactc catagacatc agtccaggtt ccctgccgtt
      241 cgctgacaga gtactgttct ggtgcagcgt agccgttgta gatctcgcat tccagttcag
      301 agtttgctgt gcggactgcc gcaatagaga aggaagtcaa aagcagatct cccttgtccg
      361 tcacataaat cgtatccgga gaaatggcac gatgtacgac gccggaattg tgcagcagac
      421 ttagcgttgt caaaagcggc ggcagcatcc gggacagctg cccccaggtg agttccccgg
      481 cgttgtcctt cagataatcc acaaaacgaa cgctgctgac atactcgtcc accacataag
      541 tggtattgtt ttccgcaaac aggtctacca ctgtattaac gtgtacctgt ccacgcaggc
      601 gtgccagcga tttgtgcagt tccgtgaatt ccgccatcag cgccttgtac tgcgccaggc
      661 gagccggaat cacgctgatg accggagaac ctgccacacg gttgcacagg ttcggcggca
      721 tatactcccg cagatagacc ttgcatccga tggactggtc ataagcaaga tatgtgatgc
      781 cctctccgtt cacgaaaagc gcaacgccaa cgatatatcg gttatttaac tgagtacccg
      841 gaataatgaa agacgggtca ctcggggact tgtcgtaata tccacaattg gggcagactc
      901 cgccagaaaa ttctgtgcgt tccatgcagc catagcataa tttccgttct cccaaagcga
      961 tacctcctgt tgattcgttc agcattcctg ccggcgaagc ttcctatctt tggcaatctg
     1021 ttcccgataa gaattccgca cattatattc taccagcaac caagcgcaat gtcaactgtt
     1081 ttccttgaaa tttcactgga ttctgtccaa attgtatcca tttgtaacaa atttgatact
     1141 gtttcttggg catcaaagcg agagcaagcc ctgttccagc atcttctgtg ctgccagcaa
     1201 aatgccgatc ttaccggtgg tataggtcat accgccctgc atccagactg caaaaggttc
     1261 acgaatcggc gcatctgcgg acagttcgat cgaggcaccc atggtgaatg cgccggcagc
     1321 catgataact ttgctggtat agcccggcat atcccatgcc tccgggatta caaaggaatc
     1381 caccggcgaa ccctgctgga tgccctggca aaaagcggtc aggtgtgcct cgtcgcccag
     1441 gcagatcgcc tccacaatgt cgccacgtgt cgtgcccaca ggcggatggc aggaaaatcc
     1501 gaatccgcca aagagagagg cggcgaaatc tgccgttttc agtgcgtttg ccgtagtgtc
     1561 cggtgcgtgg aaaaatccca ggtaaagttc ccggttcata tccagcgtgc agcccacttc
     1621 cttgcccatg ccgatgcagg tcagacgttc ggcgcactgc tgtaccagtt cttctttgcc
     1681 ggcaatatag ccgccggtgc gtgcaatacc gccgcctaca ttctttatca tagagccgat
     1741 catcagatcg cagccgactt caaccggctc gctggtttcc acaaattcgc cgtagcagtt
     1801 gtctaccatg ataacagcat ccgggttggt gctgcgtacc agttctgcca tacgctgaat
     1861 ctgtgccaca gtcagcgcag ggcgcagaga gtagccacgg gagcgctgga tgtatgccac
     1921 ctttgcccca cgaacggcat ccggaatcgc atcataatcg ggtgtgccgt ccgcagtcag
     1981 cggcacttct ttgtaggtca cgccaaagtt tttcagcgaa ccgcttttgt catcgtcgtt
     2041 cagaccgata acaccttcca gggtatcgta cggtctgccg gtcagagaga ccatagtgtc
     2101 tcccgtgcgg agaatcccga acagtgccac actcagtgcg tgtgttccgg aaacgaaatt
     2161 gtgccgcacc agcgcatcct ctgtgtggaa tgttgcagcc acaactttat ccagcacttc
     2221 tctgccctgg tcgccgtagc cgtagccggt tgtaccataa aaacacggtt cactgacacg
     2281 gcttttctga aatgcggcaa gcacttttgc cccgttatag gcggcatttt cctctatttt
     2341 ggcgaagtcc tcccggcagc gtttctccgc tgcctctgcg gctgccatga tccgatcgtc
     2401 gatcgggaaa aattcttgta tctgcatgaa ttacagcgct cctttctctt gctgttcctt
     2461 ggggatgtcc tggcgctcca gcacgtattc cgaggcgatc tcctgaaaac cgatctcccg
     2521 gtaaaagctg atgcggacat ccagggcgaa cagagccgcc tgcttgccct cagccgccag
     2581 ttttccggcg atccagcgca ggaagtcccg tgcatagccg ctgccacgct gtgcaagctt
     2641 ggtcgcaacc tgtcccacca gaacattgcc tttccagtcg tataccagag tcagcgtgga
     2701 gctgttttta taggtcagca ccttgctcat gccgtggcgg cagcgatggg aaacatctgt
     2761 caaccagagc gaatattcca gcagattggg aaagccttcc tgcaggattt cgtaaacatc
     2821 ctgtaatctg ggcgattcgt ttacttcatc cgggcagaac gccggcgatg gtgcagaggg
     2881 tgtcagatgg aatgttgcac ggtgcagctt ttgataattc gggatctgtc ccagaaccgg
     2941 gagcagttct gccgggcatt cgatgcgaaa cggcaggtgc atttccgcaa aagtgcggat
     3001 ttcttcttcc ggaacgctgc cttctgcaca gatnnnnnnn nnngtggcgt taaacaaaaa
     3061 cagccagcct gtgccgccgc agcccgttac ccggaaaaag cggcagaaat catatccgcc
     3121 gccgtaagcc tgctgatagg caagcatctg cctgccacgc cagctttgtt ccagaatcgc
     3181 tgtttcctcc ggggaaaatg cagtgatttg ttgtatcata tcgtcgaaac ttcttccatt
     3241 atggatttta ccagcagcgg cagcaatgcc gtgcaggcgt caaggtcgtt caagtctgcc
     3301 aagcaggacg gtgaatgcag atagcggcac ggcaaggaaa ttgccagggt gcgcacgccg
     3361 ccacggctga cgtggatcgc accgctgtcg tttccgccgg cgatccggga ttttgtctgg
     3421 caggaaatgc cgtttttctc cgcaagcgaa aatgccatgc ggtacagttc cttgtcataa
     3481 atggtgctgt gatccatgaa cgaaacaacc ggaccgccgc ccagacagca gacctttgcg
     3541 tcctcttctg cccctgcaat gtctgccgca gtggttgcct ccagcaccag agcaaagtcc
     3601 gggtcaactg tatatgccgc agcctttgcg ccacgcagtc ccacttcttc ctgcaccagg
     3661 aatgcagccg taaaatcata gggggcatcc tgttccagca gatgcagcag cattgcacag
     3721 ccggcacggt cgtcgatcgc tttgctgcaa atgcggtctt tgcccatgcg gacaaaatcc
     3781 ggtgcaaagt acgcatatgt tccttccggt gcggcggctc tggcttgttc cgcattgtct
     3841 gcgccgatgt ccagaaccag accgctccat ttgggcagcg ctttccgttc ttccggggac
     3901 agtagatgga taggtttggc tcccaccaca ccggggagat gcttttcgcc gaccagcact
     3961 tgtctgccaa tggcaaccga tgcgtctacg ccgcctaccg gctggatgca cagtgtaccg
     4021 tcactgttga cgtgcgtcac gatcatgcca acttcatcca tgtgggcgct gaccatcagc
     4081 ttgtgcggcg cacgattctg tccacgtttt tccaccagca gactgcccag cgggtcgatt
     4141 gtccaggtca catcgtgccg cttttccagc acggagatca gatagctgcg tacagcctgc
     4201 tcatcgccgg atacagcgca aagattacac aaggtttcaa aatgattcag catacactgc
     4261 acctcctcag ataagccgcc agcagttcgc cggtctgctt gacatcagag atctggatga
     4321 cctctgccgg cgtgtgcata taccggagcg gaatggatac agttccggta cgcacgccgc
     4381 cttttgttac agaaaaacga tctgcattgg ttccggtcat gcccgacatc acttccggct
     4441 gccagggaat ctgttccgca tctgccgcag cgatcagcgc atttgtcaca tcacgggaaa
     4501 gggtcggcga aatgccgatc ataggaccgc cgcccatttt tccgcacttg accggatcgt
     4561 ctccgtgtcc cagcgcaaaa gtcacgtcca ctgccagagc aatatccggt tcgatggtaa
     4621 atgcaccgat ggttgcgcca cgttcgccgg tctcctcctg ggtggagaac agtacagagc
     4681 agctgcacgg cagttcctca ccctgtacca gggaaagtgc gtacaatatt gctgcaatgc
     4741 cggagcgatc gtccagagag cgtccgcaca ctctgtcatt ttccataggc aggagcggca
     4801 ttgcaaagga aacggtgtca ccctgtgtga cgatctgttc cagttctgcc tttgtgtatc
     4861 cggtgtcaat ggcaagttct gtcacagaga gaacgtgttc ttctccgccc tgtaaatgcg
     4921 gcggcacgca acagatcaca ccgggaatat cctgcttgcc gtgtacagtc acacg
//
LOCUS       DAWENO010000152         4969 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6357_length_4969_cov_8.196785, whole genome shotgun sequence.
ACCESSION   DAWENO010000152 DAWENO010000000
VERSION     DAWENO010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6357_length_4969_cov_8.196785"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1186..1195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1174 a         1296 c         1401 g         1088 t
ORIGIN      
        1 cgctgaaaaa atatttcctg ggtcaggcag ttccgccgaa tacccgtgta acgacctgtc
       61 agaagtgcat ccgtaccccg gatatcgagc gtgtcggtca gaccgcacgc cacggtactt
      121 atttcgagat gctgggcaac ttctctttcg gcgattactt caagatcgag gcgatcacct
      181 gggcatggga attcttcacc aaggtgctgg agatgccggc agataagctg tatgtttccg
      241 ttttcgaaaa cgatgacgaa gcatttgaaa tgtggactaa gcagatcggc gttgccgaag
      301 atcacatggt tcgcctgggc agagaggata acttctggga acatggttcc ggtccgtgcg
      361 gcccgtgttc tgagatctac tttgaccgtg gaccggaaaa gggctgcggc aagccggact
      421 gccacgttgg ctgcgactgc gaccgctatg tagaggtttg gaacctggta ttcagccagt
      481 ttgacagcga cggcaacggt cactacaccg aaatggaaca caagaacatt gataccggta
      541 tgggtctgga acgtctggct tgtgtgatgc agggcgtaga caacctgttc gaggttgata
      601 ccgtacagaa catcatgaag cacgtttgcc gcatcgcagg cgtaacttac aacgaagatg
      661 aaaaggttga cgttgcgctc cgtgttatca ccgaccatat ccgcagcacc actttcatga
      721 tcggcgacgg cgttatgccg tccaacgcag gtcgtggcta cgtgctgcgc cgtctgctcc
      781 gccgtgccgc aagatacggc agactcatcg gcatcgaaaa gccgttcctg tacgaagttg
      841 tggaaacggt tattcatgaa aacgcctccg cttatccgga actggaagag aaaaaggcat
      901 acatccgcaa gatcgtggaa gaggaagaaa agacctttgc aaagaccatc gacaagggct
      961 ttgatatgct gtccggcatt ctgacgcagc tgaaatccga gggcaaggac atggtttccg
     1021 gcgaagatgc gtttaagctg tcggatacct atggtttccc gattgacctc accgaagaaa
     1081 tcgcagcaga gcagggcgta tctgtagacc gtgaccgctt tgcggctctg gttgaggaac
     1141 agcggaaccg tgcaagagaa gactatcaca ataaggcagg ctcttnnnnn nnnnnacgca
     1201 ccggcaaccg tattcaccgg ctatacccat aacgcagaag aggatgccaa ggtcatcgca
     1261 atcttccgtg acggcgaggc tgttgacacc gctgctgagg gcgaaccggc agttgttgtt
     1321 ctggacaaga ctccgttcta cgcagagagc ggcggacaga tcggcgattc cggttctgtt
     1381 accacaccgg acagcacatt cacggtagag acaaccagca agaccgaaaa cggacactac
     1441 ctgcacctgg gtgccatgac cgaaggcgca ctgcacgtag gcgataccgt atctgcaaag
     1501 atcgacgcag acaagcgcag cagaacgatg cgcaaccata ccgcagcgca tctgctccag
     1561 gctgcactgc gtactgtcct gggtgaccac gttcaccagg cggggcagct ggtaaccgac
     1621 agccgctgcc gttttgactt ctctcacttt gccgcaatga cacatgagga gatcaaaaag
     1681 gtcgaggctc tggtcaatga aaagattctg gaggcaatcc cggtagttac acaggaactg
     1741 cctatcgccg aggcaagaaa gctgggcgca atggcactgt tcggcgaaaa gtacggcgac
     1801 atcgttcgtg ttgtaaccgt tggcgattac tccgtagaat tctgcggcgg tacacacgca
     1861 gacaacactg caaaactggg actgttccgc atcctgtcgg aaagctcagt ggcatctggc
     1921 gtgcgccgta tcgaggcagt caccggcaca ggcgttctgg aactgctgga ttcctacgaa
     1981 gagaccatgg catctgctgc taaggcaatg aaactgagca acaccaccgg cctggcagaa
     2041 cactgcgcat ctctggtggc tgaaaacaag gcaaaggatc gccagatcga atctctgaac
     2101 cagaagattt ccgatgctca gatggctgca attttcgaca acgccaagga gatcaacggc
     2161 gtcaaggtcg tttctgcaat gctcagcggc acgacaccgc aggtactgcg gaagatgggc
     2221 gacaccatca aggacatgaa agaaccggtc attgctgttc tcgccagcgt aaacggcgaa
     2281 aagggcaatc tggtttgcgc ttgctccgct ccggcaagag acaagggcgc acacgccggc
     2341 aagatcgtac agcgtgtagc tgctgtcacc ggcggcaagg gcggcggtaa gccggacagc
     2401 gctatggcag gcatcggcaa gacctatatg gtcgatgagg cactgatggc tctggacggc
     2461 attgcagcag aatttctcaa ggaggactaa tcatggaaga ctgcctgttt tgtaagatca
     2521 tcgcagggga gatccccagc gcaaaagtat acgaagatga aacggtttat gtgttcaagg
     2581 acatcaaccc gattacaccg attcactatc tgctgatccc gaagcagcat atctgctgtg
     2641 cggcaaagat cacgccggaa aattctgcgg ttgtcggtca tatttacgaa gtggctgcaa
     2701 aaatcgcaga gcaggagggt atcacggatg gctatcggat cgtgaccaac tgcggcgaaa
     2761 atgccggaca gactgttttc cacctgcatt tccatatgct tgccggtgta aagatgggtt
     2821 ggggcgcaga cgcagtacag cccattgcgg actaaggagg tacggtctgt ggcagcatat
     2881 tatacattgc acattgccgg tctgacacgg gaactgaaaa tctgccccat cagcgaaaag
     2941 ctggacattg cggcatttat tctgttctcc gatgtggaac tgacagaggc gtgcgccaga
     3001 gaactggcaa agcgtgtgcc ggaatgtgac tatattctga cggcagagtc caagggcatc
     3061 ccgctggcat atgagatgtc ccgtcagctg aaagtgccgt acatcgtcgc acgaaagtcc
     3121 atcaagggtt acatgaccga tgtgctttcc gtgcaggtga aatccatcac caccgcagcc
     3181 gtgcagacac tgtatctgga cggcgaaaag gcggcgtgga tgaagggcaa acgtgtcctg
     3241 attgttgacg atgttatcag caccggagat tctctccgtg cgctgcagca gctggtgaaa
     3301 tctgccggcg gcgagatcgt gggcaatgcg gcgccccttg ccgagggcga tgcagcagag
     3361 cgcaaagatc tgatatattt ggaacaactg ccattgttct tcaaggattg aggacagggc
     3421 agagatgaaa cggaaaatgg tatggctggg gattccctgg ctgattgggc tgtacttcgc
     3481 agcaacctgc cagatctccg tgacgacgag tctgcttctg gcggcatttc tgctgctggg
     3541 agctttccgt ctatatcggc gtattacaac ggcacagatg ctcaccgccg gactgtctgc
     3601 ggcagcagct gtcggtgcag ttttgctgta cacaacaatg gtgtaccagc cgctgcttgc
     3661 gcacgccgga acgataacaa ccttttccgg acgagtcaca acggtcacaa cctatgacaa
     3721 tgaccgggca tcgtaccagc tgaaaggaca gttccgtgac ggcaaaaagg caaagatcct
     3781 ggtctatacc gatgacgtag gcgctcagta tggggacacg ctggatgttg caggaacatt
     3841 ttccgttccg tcggacagct acctctggaa tacaggcagc tactataagg caaagggcat
     3901 ctttctacag gcagacacca aggactacat ttcctgcaca cataccgaaa acggaaagct
     3961 ggtccgtgcc ttgcaggcat accgcagccg tatttccatg cgcatctgca ccctcgccgg
     4021 aacggatgcc ggcggtatgg tcagcgccat gctgcttggc acaaaggaaa cgctgccgga
     4081 ggaaactgac gctcttctga cgcatcacgg cattcgtcac gtggtatccg tttccggtct
     4141 gcacttggtg ctgatcctca gcatctgggg atttctatgc cggaaactgc attttcaccg
     4201 ctggctgacc ttcgggacaa cagcagtctg gacagcactg tatgcgctga tggttggtgc
     4261 gcccatttca attttgcgtg ccgggtttat gtttctgatg atgcagagtg cgccgctgtt
     4321 tttccgaaag gcagacacct gcaactctct ctgcatcgcc ggaatcctta tgacgatcgg
     4381 aaacccttat ctgattcaag acgcctcttt tctgctgtca tataccggca catttgccgt
     4441 tggcgtgttc gctccatgga tgacacgcaa gatgcgaaaa aagggattcg aatggagtct
     4501 tttccgccag ctgacaaccg tgacctgcgt ttctgtctgc atttttccgg tgacgctgct
     4561 ctatttccgg gaggtgtccg tggcgtctcc cattgccaat ctgctgcttg tcccgatttg
     4621 ttcgctgatg ctgatgctgt ccctggcaat ctttctgacc ggcggtgtcg gcatcatagc
     4681 aaagcccgtc tgcttttgtc tgaaactgct gtatgacggc atgatgctcc tggggtatgg
     4741 tctccgtacg cttatcccgg tgatgtttcc aacgggctgg aaacagctgc cgctgctgtc
     4801 gagcattctg atcctgttcg ttctgacggt gatctgtttc cggcgaaacc ggcgtacggc
     4861 tgcggcatct atcgccgttt cctttggcat acttatcgca gggcagggca tttaccgctt
     4921 gagcgaacag cggtgcttta ccgttaccgt cctgggacag aggcaggaa
//
LOCUS       DAWENO010000153         4956 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6369_length_4956_cov_9.440114, whole genome shotgun sequence.
ACCESSION   DAWENO010000153 DAWENO010000000
VERSION     DAWENO010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6369_length_4956_cov_9.440114"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2082..2091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1258 a         1108 c         1397 g         1183 t
ORIGIN      
        1 gcataagaaa gttctccgga acggtaactt ctggggcttt cgccttatct ataaaaataa
       61 ggcagaatta agtaggctgt tgtcggcata cgctccattc aaccgcttgc agagtctgta
      121 cagcacaata aatttttcaa aaatagttga cagtatcaga aatctgtgat atactaaaac
      181 tagctaagag atattttgta gtgctgttag acacgcaaaa aaagaaagcc cccagagctg
      241 caactccggg ggcttttgtt ttagggtgtg tcgggcttat gttatctgtt cacccatttg
      301 tagagattgt agctaataat ctccaccgta acagacacca aaagagatat taagtaatac
      361 agcgttagac acctcctccc tgacggaaga agccgacaac ctatcataac acacagaaac
      421 ctttttgtca atccgacaga gacgatgtcg gatcgtgata cattgtgaaa ggagcattcc
      481 atgatgctgc accaactgac ctggcaggag atctgccgga tgatacccaa acgaaaaacc
      541 gatgcccaga aaggcgattt cggacggctc ctgtgcatca ccgggagccg gaatatgccc
      601 ggcgcctgtc tgctttccac acaggcggca ctgcgtggcg gcgccggtct tgtcacagtg
      661 gcgactgcgc cggagaatcc catgcgtttg gcagctgctg cgccggaggc gatgtggctc
      721 tcgctggaga cagacgaaaa cggctttctg accaacagcc ggcgcaaccg ggaacagctt
      781 gaaacgcaac ttcgccgtgc caacggggtg ctgctgggct gcggcttagg ggtgacggac
      841 gagacaagac agctggtgcg ctgggtgctg gaaaatgcgg cgtgtccggt ggttttggat
      901 gcggatggtc taaactgtgc ggtctcctgc atagagttaa gcagacggac ggggacgaac
      961 tggatcctga caccgcatcc gggcgagatg gcacggctca ccgggcagac aattccgcag
     1021 atacagggaa accgtgcgga aacggcgtct cggtttgcgg cggcgtatcc ggtgacactg
     1081 gcgctgaaag gtgcaggaac actggtggcg cagggcgaac ggctggcgca gaatcccacg
     1141 ggaaatccgg ggatgagccg tggcggcagc ggtgacgtgc ttgccggact gattgtagct
     1201 tttgcggcgc aggggctttc gtcctgggat gcggcttgtg ccggcgtgta tctccacgga
     1261 ctcgccgggg actgcgccgc tgctgcgctt tctcagcagg cgatgctccc acgggacttg
     1321 ctcgcctatc tgccgcaggt gttggcaaag ctggaacagg agcggtaagc cgcagggtgt
     1381 ctccgtctga tgacacagga ggagattcat atgaaacgaa aatacagagc agttctgggg
     1441 ctgatccttg ccggaggcgt ttgtctttcc ggctgttcca agccgggcgg cgaggcaagc
     1501 gcagcccaga aactgggcgg aagtttcacc acggagatga ccatgcagat ggaggactgg
     1561 accgccgcag gcaccctgtc ccggctggga gacggtgcgt ggagcgttgc ctttgcagag
     1621 ccatccacgc tcgccggggt ggtgctggac ttttccggcg gcgaggtcac ggcatcgtat
     1681 aaggggctgg gtttttctgt gccgcagtcg gcaatgccgg caaagtccgt gctgagccag
     1741 atgatcctag tggtggatga actggcgcag caggaggaga tcaccgggaa ggagaaggac
     1801 ggcggcgtgg cagtcgaggg cgagtcggag ggcaatccct atacgctgac gctgacggaa
     1861 agcggcgatc tggctggctt ttcgctggaa aacttcgatg ccgaactcac attcacgcag
     1921 tttcaagccg gggcagatgt ggcaacggag atcacaacaa tggaataaaa aaagacgaaa
     1981 gaagtcgccg taccgtgata aactgtacgg cgacttttta attttttcgc tgcaattcat
     2041 gaaatatttt tatgttcgat gaactgcatt cacagggata tnnnnnnnnn nggatataaa
     2101 ataaaaatat gggagaaatc ttccatatgg aaaaggggta attttcatgc aaaagacacg
     2161 cagatttttt tcgaaactga ctgctgcggt gcttgccggt gcgatgctgg tttccggcgg
     2221 ggcagtttcc atgacggttt ccgccgcatc tgcaaagccg gttctgaaca cggatgttac
     2281 agtgccgtcg gcaggcaatg ttctcatcgg gatggaaggc acctatgcca atgacataaa
     2341 ggcagcgctg gatttggtga atcagtatcg ccgggaggct tgcaacaacg gctatcccaa
     2401 tccggtggac ggcaaaaagc tgacgccaag cgactatgtc cccatgcagt ggtctgccgg
     2461 gttggaatat ctggcacgaa tccgtgcagc ggagagttct cagacgattg cccacgaacg
     2521 caccaacggc tctcactgtt ttaccattca gacgcccggc ggctatagtt ccggcggcga
     2581 gtgccttgcg tggtggagct gggggctgga tatgaaatcc ggtgtggaac tgtggtacag
     2641 ggaaaaggac gcctatctga acaaaaccgg cggcataacg ggacactata ccagcatgat
     2701 ctcacccaat aaccgctatg tgggcatggg cgcatttggt acaggcactg cgcagtatgc
     2761 cagcacttcc tgcgccgagt tcagcggcac caacaccagc gagacgttcc agttcaccta
     2821 cggcgactgc atacagacgc tggaagtgcc ggcaaatgcg gtgacgatct cttccaacca
     2881 gagcaagtat ctctactacg gcgaccggaa aaccttacag ttgacggcat cttataacgg
     2941 aaacagcggc tttccggtgg agtatgccaa gtggagttcc tctgcgcccg gcgtggtaag
     3001 cattgagggc gacaacactg ctgtggtaaa ggattacggc aaggcgacgc ttaccgcaga
     3061 cctgggcggc aagacctata cactgaatgt agaagtaaaa gatattgtca aaggcgatgt
     3121 ggagtgcgac ggcaagatcg acagctccga tgtgttcagc acgctgctcc atgtggcaag
     3181 cgttggcgcc ggtgtcggcg gaacgctcac cgacgggcag ctggccgcag cagacattga
     3241 cggagacggt actgtcaatt ctaccgatgt gtactatttg atgtattaca ttgccttgaa
     3301 cggcgcagga attcacagca cgtgggaaga tgtgctgcaa taagaaaaat aagtacacca
     3361 cttcccgaaa ccctataaaa aactgaaacc gtgtgcgaaa caatttgcac acgatttttt
     3421 tgtgtcaaaa cggtcaaaat tccacagttc acaaaatatt ctcaaaaagt tgtttctttt
     3481 tttttttttt tttttgcgtt ataatagaca atgttaccat ttatcatgga taactatgca
     3541 gaaacatcat ggtataattg tgaaaggagt aaacgaggat gcaaaaaatg aagaagtttt
     3601 tggcggtatt gacggctggg gtgctgtgtg ccggggcgct gccggtgatg caggtgaacc
     3661 tgccgcagat gtccattacg gcgagcgcag agactgcgga cggactgagc taccagctca
     3721 tcaatgacga cacagaggta gcgatcacag gctgtgacgg cgatatgact tcggtcaccg
     3781 ttccgtcgga gatcgaggga aaaccggtga cacaaattaa cagttacgca ttttcctcaa
     3841 aatctaaatt gctgaaagtt acaataccgg acagtgtgac ttccataggt aacgctgttt
     3901 ttagtagctg tgcgcacttg agaagtgtga ctatggcaga cagtgttgaa actatgggat
     3961 actctgtatt taatggatgc tctgaattga aagctgtcaa gttctccggt agcttaacgg
     4021 aaattgcagg ctcgacattt tatggatgca gtaatttaac agaagttgcg tttccggatt
     4081 ctgtaacttc cataggcaat gcggtatttt atgattgttc acggttgaaa caggtaacct
     4141 tgccagaaaa tttgaaaagc atggggaatg aagtgtttta tagatgctct gcattgaccg
     4201 ctatagacat tccagactcg gtaacgacta tgggagaaag tgtgttttat tactgcactg
     4261 cgttggaaca aatctccatg cctagcagct tgacaagcat gggcaacagc tttttttatg
     4321 gttgttctgc tctgacctct gtggctgttc cagagggtgt ttccgttttg gaaatgcata
     4381 cttttgataa ctgtaccaat ttggcagaag taacacttcc tagtaaattg gataaaattg
     4441 agaacgaagt gtttcgtaag tgtacttcat taaaggcgat agcactgccg gaaggtttga
     4501 ctgaaattaa agaatatacg tttaacggtt gcgagcaatt gacagatatt gtgattccgg
     4561 attctgtagt ttccattgag actggtgcat ttggagattg caagagcctg gaacaaatca
     4621 caattcccga cagcgtgttt agtttaggag aatctgcatt ttctggctgt tcttctttga
     4681 aatctgttac aatttcgaat ggtattatgt ctttggaaaa tagaactttt tcgaattgtt
     4741 ccagcctgga acaggtcacg gttccagagg gtgttactga tttaggaaac tatgttttca
     4801 gtggttgttt gggattgaaa caagcaactc ttcctgatag tgtagcgaaa atcggagaat
     4861 atgcttttga aaactgtaat agtttggaac agatttcgat tccggatagt gtgactgatt
     4921 tgggaaaaag tgctttcagc ggttgtactt ccttga
//
LOCUS       DAWENO010000154         4952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6379_length_4952_cov_8.253420, whole genome shotgun sequence.
ACCESSION   DAWENO010000154 DAWENO010000000
VERSION     DAWENO010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6379_length_4952_cov_8.253420"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1067 a         1298 c         1182 g         1405 t
ORIGIN      
        1 gttcctggag cagcacagga gctacctggc tgcaaacaaa catctgtgcc attttatcgc
       61 tgattaagat gtcacctatg gtatcccgtt ccaggttgca cgccatgcaa gctccgagaa
      121 aatcccggtg tgccggcggt tttggaacac gataagaaaa cgtcagacac tgtatcggga
      181 actgctccgg ttcgaccggg atgaattccg gcgcaaagca gagcatgaca cgtgtggtgt
      241 tttctgttcc gccccatgcc gtgcagacag cgccggcgga acgggcaatg ctttctgccg
      301 ccgggacatc ttccaccggt acaaaatccg taaaatacgg atggctgtcc cggatacagc
      361 ggcttgccag atcgctccag tgcgcccgga actgctgcat tgtgtccgac gacttcatat
      421 tgcggcttgc catttcggaa gtagcttaaa ccagaccgct gccctgcagt tcgccgacaa
      481 agctgtcacc gtcaaagccg acattctgcg gaatgatcag aaatgtctgg tttgcgatgg
      541 gcttgatgcg accgtgcatt gcgtaagctg cgccaacaac aaagtcgatg atgcggcgct
      601 tgtccggctc ttcaatgcct tccaggttca gaacaaccgt cttgttctgc atcagataat
      661 ctgcaacctt cttggaatca gaggtatacg cagttgcttt taccagaacg acctggccct
      721 gtgcagttgc cttgatcgtg gcatatcttt cgttgttctt gcggtcatcg taggaagaag
      781 ttctggtata tccggcattg ctgtagctgg atgatgcagt tgcagtggat gcagccgccg
      841 gttcttcctc cggtgctgtc atattcgggg cctccggctc attgtcatca gccggcatga
      901 aaaagtcttt caaactatct aatacgttca tgatactctc cattctccgc acaagttgat
      961 attgttgaca ttatcgtgca gtggtgcggg tacgacaatg ttctttcatc acacacatca
     1021 ggatgagcgg atgttattta cttttgataa tttcttgcgc cgaacagggc tgtgccgatc
     1081 cggacaagtg tcgatccata gcggatggcg gctgcaaaat ctcccgacat tcccatcgac
     1141 aaaatctcca tatctatatt atccattttt ttggaggaaa tgtcaagata aagttgttgc
     1201 attttgtaaa gaaatttttc atcaccctct accggcggga tagtcatcag tccacgcacg
     1261 tgtacgtgag acaactgtcc tgcctcgtgc aacagttctt ccagctgatc cggtgcgatg
     1321 ccgctttttg aagcctcacc gcccacattg acctcgatca ggatgtcctg caccttgttt
     1381 acccgtgcag catggcggtc aatttccgtg gcaagcttga tgctgtccac agagtggatc
     1441 aagtccatat cctcgatgat gtacttcact ttattggtct gcaagtgtcc gataaagtgt
     1501 acctttgcat ccttggaata gaaatccttt ttggacaggt attcctgcac ccggttttct
     1561 cccagggtat gaatgccgca gcttatggcg tggttgactg cctccggtgc aacggttttc
     1621 gtcaccgcca tcagtgtaat gtcatcagaa ggcttgcggt atttcgcttt tgcctcctcc
     1681 attgcatcga cgatccgctt gtagttttcg tcgatataag caaagttagt ttccatttgc
     1741 atcaatccct tctacaatta tggaatcata aagcattaaa taaccatcgc ctgcgttgag
     1801 ctttgagaga acataatcat ttccctcata aaccacatcc agacgccgga attcgatttt
     1861 ttcgccgtcc tgtacataga caccacggca attgacggtt tgctttttct cttctcccgt
     1921 ctcttcgtct gtgaccgtct cttccacatc cttgaaccgg atcgcatttc tcggaacctt
     1981 gatgccctca tattcgccca gaaccatttc cacgttttcc gtgcggttct gcacaaaatc
     2041 ggaggtcaaa gtgtcgctgt acagcacaac catgaacttg ccgtcttcgc tggtgctgcg
     2101 gagttctttg atggtggcat ctgcggttgc agaagaagtc ataagacgaa ttttaacaga
     2161 atctcctacc tgatatttct gttctttatt atcaatgatg cccagcatat accagccata
     2221 ctgtgcaatg gtctttccga caacattgct gttctggctt gtctgggtcg tattgtcatt
     2281 actgtttttt gtgatctcct gtatctgttc cggcgtgatg ttcgccagat tttcgatact
     2341 gagcttggat tccagcccgt ccacgtggct gacaaaatag gcagagctat cagattttac
     2401 cgtgtctacc ggtgtctcct gttcgctttg cagcgcctgg atctgcgctg tcagatcgct
     2461 gatctcctgg gcaaaggaaa cttcaccctt gctgattacg atttgatatg tgctgtagga
     2521 ttccacaaac tgctgctgtg cggaagataa ttccgtcaag tcaccgctgt tccggctctg
     2581 cacgatcttt tcgtaatact gtgagatctg ccgggagagg tctgccggct gcgcctgttc
     2641 cagcgtgccg ggatttgaga tgcgttccag cacgttcagc ttatcttgaa gaaccttgat
     2701 ctgctgttcc ttttcgattg cgctttcatc cggatacact tctgcgatca catcgtttac
     2761 ggcaatttta ccgccgtccg gcacctggta gctgacagca cctgttccgc tgtaggacag
     2821 aacattttca tcccggacat acacgccctc aaaatatttg gactcgtctg cggtggcacg
     2881 gatcgccgtt tccgtttcat agctggtatc cacaaaatga taaaagatcg tgccgatggt
     2941 tgcaatcaca gatgcagaca gcagtaccaa cagaacatta ttcattttat tgctggaatt
     3001 catacctgga ttacctctca aaaaatgatt tctccgctgc ggtcagattt cgattactct
     3061 tctcccactt ccagaatcga gatggggtga atcggaatga tcgttgaaat ggcgtgcttg
     3121 tagaccagat tctgcttgcc gtcgcattcc aggatcacga catagctgtc aaatcccttg
     3181 acgacgcctt tgaactggaa tccgttggtc agaaaaatgg tgacgccgat tcgttctttt
     3241 cttgcctggt tcaggaacac gtcctgaaga ttgatgttct tgttcattgc tatactcctt
     3301 tttcttctgc agccagagcg gctgcgtgag cgcagcagac ttttccggtg tttccgatac
     3361 agtctgccct tattttctct ttgtatataa tggctcaatc tatattataa cacatttcgc
     3421 cggacttggc tataattttt tgacactttt ccataatttt ttgtttcttg tcgaatctgt
     3481 ctagaataat ccactgtatt tgattgtttt tgcggaacca tgtcaactgt cgtttggcat
     3541 atcggcgtgt ttcctgtttg attttgtcga tgcacgtctc cagagacatg acaccgtcaa
     3601 aatatgggat cagttctttg tatccgatcg catgatacgc cgtccgcagc gcctcattct
     3661 gatacacggc atacgcttca tctaccagtc cctgtgcaac catctgatcg acacgctcgt
     3721 tgattctgtc gtagaggacg ctgcgctcgt acgtcagccc gatcaccaga gagcggtaag
     3781 gactggggat ctgatggctc tccgctttgc aggcagtaaa tgtttttcca gttagcgtgc
     3841 agacttccag cgccctcacc acccgaacca gattgttcgg atggatctct gccgcagcct
     3901 gcggatccag tgctttcagc ttgtcgtgca gggcatcgtt tccatgctcc gcagcgtatt
     3961 gcagaaactg ctgccgcagc gccggatctt gtttggtagg ggcaagctgg acattggaaa
     4021 gcagcgaaga aacataaagc cctgtaccgc ccaccacgat gggcagatga tttcggtcgt
     4081 gtatctcctg aatcagcggt ttagccagag acagatagtc ggcaacgctg aatgcggcat
     4141 ctatcggaat acagccgatc agatgatgcg gtacgccgtc ctgttcttcc ggcgttggtt
     4201 tcgcagtggc aatatccagc ccttgataga tctgcatgga atctgcagag atcacctctc
     4261 cgtggtattg ttttgccagc atgacgccaa gcgctgtttt tccggatgcc gtcggaccaa
     4321 caaccgccag caccgggagc ttttccgatt ctgtcatggc ttataccctc cgtcggaatt
     4381 gtttttccaa gtcatatttg cggatgacga acatcaccgg tctgccgtgc ggacaatgcc
     4441 ggatgttttc gtcatcccag acctgctgcg ccagtttttg cagttcctgg atcgtagaat
     4501 ggtcgccgga acagatggcg gatttgcagg caatggtatg caatgtatcg ttaaacgtat
     4561 gcacctgggg atcacgcttt cccagtacca gattgtgtgc gatttctgtt accacttcat
     4621 cgatgttcag cggcaggaca aaggacggca cagcagtcgc ccggagctgc ggcggtgtgc
     4681 tgcagtcaaa ggcaaagccc atctccgcca gccgtggcag gttttcctgc actgccgaaa
     4741 actcctccat agatagcagt gtctcactgg gacggagcag catctggctg gtctgcttgg
     4801 atgcgccaga acgcagctgt tcaaacagga ctcgctcatg agcggcgtgc ttgtcgaaaa
     4861 tcagcagctt gtcttcccct acgccctcag cgataatgta gttttcgaaa atctctccca
     4921 gcacacggat cctggtatgt ttcttcggcg ct
//
LOCUS       DAWENO010000155         4924 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6418_length_4924_cov_11.635860, whole genome shotgun sequence.
ACCESSION   DAWENO010000155 DAWENO010000000
VERSION     DAWENO010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6418_length_4924_cov_11.635860"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          998 a         1271 c         1455 g         1200 t
ORIGIN      
        1 gtagaagtcc acgtcaccct ttgcggagat gtccccggaa actgcggtgt tcagcggcag
       61 ttcctctgcc gtggcgatgg aatcgttttc gccggctgtc ccggcggatt ccgcatccac
      121 ggcgagggga gcgctcagac agctgatgca gagcatcccg gcgaatatgg cggcaaatcg
      181 tttttgtttt gcggttttca tgtgtggtat cctttctatg tgctgcagat ttcatggtga
      241 tttctgctaa cagtatacca tagaaaggtt aattcgcaac aaattatggc gttaaatgtt
      301 cgtaaagcga aaaataccgc acgacagcgc ctgcaatgca gcgtttgcct tgtgcggtat
      361 cctctgttgt attgttattc tgtgccggaa atggctgtct ctgcggcggt ggtctcagtc
      421 agacgctgtt ccagcgccgg aaatgccggc gggacaggca ttgcatccag cagaggacag
      481 agcgcattca gcaatgcagg ataggcggca tttcccagat gcacaccgtc tccggcagag
      541 tacatttcag ccagaccgcc ggaggcaggg tcgcttatca cggcatatgc gtcaaagtaa
      601 gcgacattct ctctgttcag ctgctgtatc gtctgtgcca gggcagcgtt atactgctga
      661 atgcttgcgt tgctggtgaa gtctgaagag ggcaggatgg gtgtgatccc ggcaacgatg
      721 atatcgctgt ccgggcagat ggtcaggatt ttttcgatct ctgcggcata ctgcgcggtg
      781 tattcctctg cactgagcag attgatgtcg ttcatgccca tggataagta gatgtacggc
      841 ggctgttttt cccggagcac ttccaggata tccttttccg cgccgctgct gtctgcaaag
      901 gtgaaatcgt gaatgttgcg gatggcaacg cacccctttg ccagtccgtt ttcctccggg
      961 atggcaccgt acagggaaaa accggaggca atcgaatccc cgactaccgt aatggcatgc
     1021 ggatagaccg catggcgctg ctgcagctgt tccggtgaga gcgttgtgac cactgtggtg
     1081 acaactggcg catttgccgc ctgcacatcg ggttttccgc cttttgcggc acagcggatc
     1141 agcgagatca gcagaaacac aatgagaaag agaagcagtg ccgccaggat catgcggtca
     1201 tagcgcatat gccgttttcg aacaggtgtt ggagcatcct gtgccggaat gggaatgttt
     1261 ttttgccggg ctgactgctt ttgcagcgtg ctgagatggt ttcgcagatt ttccagtgct
     1321 ggttttgcat tggaaaattg ttttttcaca aattttacga atttttccag ctgtcgggaa
     1381 atactgtccg gcagcttttt cttttgcatt ttcatttttg ataccccatt ttccctatta
     1441 tcataagagt ttgctgacgt gccttttaca cgtctgaatt atcagggcag cgctgtacaa
     1501 aacacaccga acagctttgt gcagtgctgt cttggaaaat cgttttcacc gattatgaaa
     1561 acaatcctgc gcccagcata gccagcccca ccagccctgc gccgacgcac agcagcgtga
     1621 tgcctgccac gctcgccttg gataccacgt ggtcgatgta ggcacaccgg gggtcggttg
     1681 ggtcgtagac tacgggcagc ttgctgcccg gctgccaggt ctcccgtgcc atttcctcca
     1741 tgctccgggg atgatgttcc agccgggcgt gaaagatgtc gttccgcacg gcgatgcagt
     1801 tcttttggtc ggcatcgttc cggaaaagat ttgccgatct ggaaacggtc gccacgcctc
     1861 tgtagtggag acgggcggta tagcttacac cgtccacttg aaacgatgcc accggggcaa
     1921 tggatggacc gccgctttgc tttggctggt agtgatagcg tatcacagtt ccctctgtcc
     1981 gggcgcagca gcgccgccgg agctttcctg tccgtcggaa aaaggggatg gagagcaggg
     2041 cagtgaggat gcctgcgccg ccgatggcaa ggcaggcgat gaggttgggg gtcagtgtca
     2101 tggatgttca cctacttcat gtcagatttg tcgatgagat agcttggctt cacgaaaagc
     2161 cttaccagtt tcgggtattt gtctgcatgt cgcaccacat agctgaacac cagcacagac
     2221 agcagccaga gtacttcgaa accgcctgag aggagtataa gttcgtttcg tgcctttaag
     2281 gtgtcgtggt tataattcgc cagagaaaga taaacatgat cggcggattc cagctgtatg
     2341 atcggaatca agtctttctt gttctggtca ctttttggaa aagacacaat aaatgtcact
     2401 ttgtccgcaa tttcctgttc tatctgcgcc atttttgccg aatcgggttc gattccccag
     2461 gtgtagaaag cggcagtgta tcccggcagc tccagttcca gccgcttgtc gccgtcatca
     2521 ttttctgtga actggtagga cgtaactgta caggtttcct ggtacagatc tttcggagaa
     2581 acggcacggt ctaagtgcag caaaaacagc gccgctgcca ccggtatcaa gccgatcaga
     2641 ataaagataa agacaaattc accgggattg tagacatttc cgccgaacag ttttgcctgc
     2701 tccggtgtga gaaactcggc atccggggcg tgcatccttg caatatgggc gaattttttt
     2761 ccattgcccg ccatctggtc aatgagaatg atgcggtgtc ccacgtggat ggtgacatct
     2821 ttgccgcacc ggatcttttt gatctggctg taggaatagg tgtgagagat ccgcagcata
     2881 tcccggtagg taaaattttc cggcgtgtag gtgattttct ggttcaccgt gaggattagt
     2941 accagtccca gcaggcagaa cagccccagt ccgatcaaca cagggatgcc ggcagactgg
     3001 tacaatgcga tcagaaacag aatgaaaaac aggagaaaat cgacagcgcc gaggaaagga
     3061 acataaatgg ggatctctcc ggcagtgccg taatgccatt tcgggttctt tttccggtgt
     3121 ttgtttcgtt ttgccatgat gttatgctcc acagattcgc aggatcgccg ccgccatcat
     3181 ccgggtgttt tcctccagct gttccagcgg caggaactcg tcgtctccgt gcatatggta
     3241 atcccagccg gggtattcca tgccgaacgc tacgccgccg ggaatgtcat gcacataggt
     3301 gccgccgccg atggcaatgc actcgcccgg tgtaccggtc tgttcctcgt agaccgccag
     3361 caggtcctgt acaaacgggg aatctttcgg ggtatggtgc ggatcgctct gaatcagcgc
     3421 ctcgcactgg aatccctttg cggcaaaggc agcctgtacc tgttccagaa cggtctcctt
     3481 ggtggtgcag accgggaaac ggatgtccac acagccggtg agcgtgccgt tttccacgtg
     3541 gagcatactg caaatgcagg tgagtgcgcc ggattccgtg tcggcgcagg cgatgcccaa
     3601 accgctgccg tcggtgcagc cgtgggggaa cagctgtgcc agcgcccggc agtcggcaaa
     3661 ggcgctgtca ctgcccagat aggtcagcag tccggtgatg gcgttgtcgc cgctttccgg
     3721 cgtggatgcg tgggctgcaa tgcccttgta ggcgagttcg tttccttggc gggtaatttg
     3781 actgccctgt tctgctgtgc cgcagaacgc cgcagaaagg ctcacagcgg catctgccgg
     3841 aacggcgttt ggcgcagtgc cggcggagaa ccgggcgatg gggtcggcgg tcgctttggt
     3901 gaactgtaac cggagcattc ccttttccag gtggatgatg ggatagctgc cgtccggggt
     3961 gaacacctgc ggcggcatgg cgtggcgtgc catgtaatat tccaggtcgg ttgagccgtt
     4021 ttcctcgttg cagcccagca ggaaccggac gtttttccgg agcggaatgc cggattcccg
     4081 gatggcacgg agcgcataca gcactgccac agccggacct ttgtcgtcga tagcacctct
     4141 gccgtagatt ctgccgtccc ggatctctgc ggtgaacggg tcggacttcc agttggacgg
     4201 cacagccggg acaacgtcca ggtggcacag gatgccgaga tgctggggca gggatgcgtc
     4261 ccagtcggcg gcgcccatgt ggtattcata gttggtgcag gtcatgccca gttcttccag
     4321 ctggtgcagc ataaagtcca gggcatctgc ggcaggcttg ccgtagggat agccatcctg
     4381 gggctggggt gcggcgacgc tggggaatcc gatgagacgg gacagagtct ctacagcgtc
     4441 ggcatggttg gtgtgaatgc ggttgcagac tgcttctggc agcatagtga atccttcttt
     4501 ctgtatgtag gaacacggcg caccgtgttc gaaaacttag aaactttatt gtacctggtt
     4561 caggatcgtg ccgtcttgga agtaagcatc cagattttcc agggtgactt gtgcgatgtt
     4621 gtgcagtgcc tcgttggtga ggaacgcctg gtgtgaggtc agtaccacat tgggcatgga
     4681 gaccagcagg gagagcaggg tgtcccgctt taccatctca gagcggtcct caaagaaaaa
     4741 ttcggtctct tcctcgtaga catccagtcc ggcgccggcg atcttgccgt ttttcagcgc
     4801 atccagcagc gcctcgctct ccaccagcat tccacgggag gtgttcagca ggtatacgcc
     4861 gtctttcatc tgggcgatgg tgtcggcgtt gatgagatgc cgggactgct ccgtcagcgg
     4921 acag
//
LOCUS       DAWENO010000156         4917 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6427_length_4917_cov_7.540930, whole genome shotgun sequence.
ACCESSION   DAWENO010000156 DAWENO010000000
VERSION     DAWENO010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6427_length_4917_cov_7.540930"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1598..1677
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3270..3279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1140 a         1270 c         1281 g         1136 t
ORIGIN      
        1 catgccgacg ctaagacatt tcgttctgcc gcaggaatca gtcaggatgc cgtcctccat
       61 gatttgcagc aaaatattgc acacatccgg atgcgccttt tcgatctcgt caaaaagaat
      121 gacgcagtag ggtctcctgc gcacccgttc gcacagcatt ccgccgtcct caaaccctac
      181 ataaccggga ggcgctccaa tcagcttgga gacagtgtgc ttttccatga actcggacat
      241 atcaatgcgt atcaggctgt ccttgtcatc aaacaagtgt gccgcaaggg cgccggcaag
      301 ggctgtcttt ccgacaccag ttggcccggc gaataaaaat gcgccgatcg gtctcgcctt
      361 gtcctgcaat ccggtgcttg cccggaacag ggcggctgtc agcgtgtcca ccgcatcatc
      421 atgccccacc acctgttttt tcaaggcatc gcccagcgtc agaagttttt gacgctgggc
      481 ctcggtgatt ttctctgtgg gaatgcctgt ccggacagag acgatcaccg caacatcttc
      541 ctcggatact gccgccggcg cagtcggctg tgcctccgac tcccagcgaa tcctggcacg
      601 ggaacacgct tcatctaaaa gatcgattgc cttatccggc agaaaacggt cgtggagata
      661 gcggtcggca taatggatcg ccgtgtgcag tacatcatcc gaaatctcca cccggtgaaa
      721 ttccgcatag gtctcccgca gtccctggag catggcataa cactgttccg gggacggctc
      781 ccgaacagtg accggctgaa agcgccgttc caatgcgctg tccttgccga tggtcgcccg
      841 gaactcttcc ggcgtcgttg caccaatcag gcgaatctct ccacgtgcca gctgcggttt
      901 cagcatattg gcggcatcaa tcgccccttc cgctgcgccg gcgcccacaa tggtgtgcag
      961 ttcatcaata aataagatga tgtttccctg ttccgttgcc tctgccagac agtttttcag
     1021 ccgctcctca aaatcgcccc ggtattttgc tccggaaagc agtgccggca aatccagaga
     1081 aaacagctgc atctgctgta aggactccgg cacatcaccc tgcaaaattt tcttggcgat
     1141 gccctctacg attgccgttt tcccgacccc tgcctcgccg ataagacagg ggttgttttt
     1201 ggtgcgccgg gagagaattc ggatcatctg ctgaatctcc ttttctcttc ccaccagtgg
     1261 atcatagcgg caggggcttg caggatcggt catcagcttt ccgtactgaa acagggcagg
     1321 gcagttcttc cgggaaaggg tatccacccg gtgccgttcc atccgcacac cgtatgctgt
     1381 gtcacagctg cacaaggtgc agagctggtt cagactgacg cccagctttt tcagaatagt
     1441 gactgcactg cagcatggcg catgaataat ggcaaggagc agatgctccg ttccagcaag
     1501 actgcgtccg ctgtctctgg cacgttcctt ggcatcctct aaaatggaaa ccagggacgg
     1561 cgtaagacag tcctcttgta cctgtgccgg aatgccannn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     1681 ccgccagagc gcttgccgct gcattgccgc cttcctccat cagtcccagc agaagatgct
     1741 cgctgccaat ataggtatgc cccagttctt ctgcggcatg gattgcctct ttcatggcaa
     1801 ggatcgcctt cccggtaaat ccttcagtat atcgcatagt tgtcgttcct ttctctgtat
     1861 gcagcacagc ggaaagaatg cctgctgacc ccgtgccgtg ctgctgtctc tcttttgaac
     1921 aggacaagta taccacattt ttttcgcagg atttgtcgaa ggagtcacac tttttcgtct
     1981 ggggcatatg atgtactatg aaacagacaa aaaacagttt gtttcaaata aaattgacac
     2041 aaaggagtcg tgtattatgt gtttcgaaaa taatggtgga tgctgctgga tcattattct
     2101 gctggttatt ctgttctgct gctgcggtgg cggcaacggc tgtggctgcg gtggcggctg
     2161 caacaatggc tgcggcggat gcggatgctg agaagatcag ctgacaactg aatcgctgtg
     2221 aaacgggtgc agagtctttt cgggtctctg cacccgtttt ttgttggatt acccatgatc
     2281 tgcgtcatat ttttgccgca aactttatgc agcgctgccc ttgcatttca atttgaaatg
     2341 tgttataata gagtttatgt aaaatagcgg tactgtgcag tactgccgca gacattgcag
     2401 ggaatccctg gaatgcaagt atcaacagat ctgctccgtc agtcatgccc ggaaagatct
     2461 ggtcaaagaa aggcaaatgc aatatgaaaa gacgtggttt gaagtttttg gcactgagca
     2521 tcagtgcggc acttttggta ggaacactgg ctgcctgcgg atcgaaaaaa gattctgcaa
     2581 gcaaaagctc cggagataat tcctttgtca tcaccctgta tccggacgtt gcaccgatca
     2641 cctgtgagaa ttttgaaaag ctggtcaagg acggctttta tgacggtctg aaattccacc
     2701 gtgtggtaga ggacttcatg gcgcagggcg gtgacccgaa gggtgacggt accggcggca
     2761 gcacggacac cattaaaggt gaattctctc agaacggcgt agagaacacc ctttcccaca
     2821 cacgtggcgt ggtatccatg gcaagatcca atgatccgga cagcgcctcc agccagttct
     2881 tcatctgcta cggcgatgaa agttttctgg acggacagta tgccgctttc ggtaaggtca
     2941 ctgagggcat ggatgtggta gattctttcg taaaggtaga acgttccatg ggcagcgacg
     3001 gcgcagtttc ttcaccaaac acggatatta tcatggaaaa tgtaaaaatg atcgacgatg
     3061 actccgacgg aaatccccgt gtagagatca ccatgaatga ctttctgaaa tgattggaga
     3121 acgtggaatc cacgttcgta aaaatccggc agcaggcacg tatcccattg atcgtgcctg
     3181 ctgttctgtc cacttgctga ggaggaacaa cgatgcacag cattaagcat ttttttgaaa
     3241 ccatgcagca gagccgtgtg gcatttatcn nnnnnnnnna tttatcggaa ccggtgtgac
     3301 caacggagat atcatccgtc tgtttcgcaa gaaaaacatt gatgtcacac tgctggaccg
     3361 caagaccccg gaaaagctgg gaaaagtata tgaggaattt caggcactgg gcgtaaaatt
     3421 ctgcctgggt gacagctatc tggatacact gaccgattat gatgtggtat tccggtcgcc
     3481 gggtatgtac tatcacaacg aaaaattgca ggaggcacgc aaaaagggcg ttgttatcac
     3541 ttctgaaatg gaagcctttt tcgcactctg cccctgcaag atctacgctg tgaccggttc
     3601 ggacggaaag accacgacta ccacactgat ctccgaattc ctggcacacg ccggtttccg
     3661 ggtacataaa ggcggcaaca tcggacgggc gctgctgccc atcattgagg atatccggga
     3721 agaggacgtg gctgttgtag aactttccag tttccagctg ctgtccatgc gccagtctcc
     3781 ggatgtggca gtcatcacca atattgcacc gaaccacctg gatgtacacg gcacgatgga
     3841 agaatacatc tacgccaaga caaacattct ggcgcaccag gacgctttct ccagaaccat
     3901 tctgaatctg gacaacgagc cgaccatgaa tctggcatct atggtgcgtg gcacactgac
     3961 cacattctca cgccgtcaga aaccggaatt cggcacttac ctccgggaag acggaatgct
     4021 gtgctttacc aaggacggcg tggacactcc tgtggtacag aaagatgata tccgcattcc
     4081 cggtatgcac aacgtggaaa actacctggc tgcgatcaac gccgtctggg gaactgtctc
     4141 cgtggaagac attcagtatg tggcaaaaaa tttcggcggc gtagagcatc gcatcgaatt
     4201 tgtccgggaa ctgaacggtg tcaagtggta taacgacagc atcgccacca gcccgacccg
     4261 tgtactggca ggactgcgtt ctttccagca gaaactgatc gttattatgg gcggctatga
     4321 caagaagatt ccctttgagc cgatggcaga taccgtttgc cagcaggtaa agctggtgct
     4381 tctcatgggc gttactgcgg ataagatcga ggcagccatt cgaggcagcc agtactataa
     4441 ggaaggcgca ccggagctgg tacacgtcag ctccatggaa gaggcagtgc aggaggcgca
     4501 gaaacgtgcc aaagccggcg atatcgttac actgtctccg gcttgtgcaa gctttgacct
     4561 gtacccgaac ttcgaggcaa gaggcatcca ctataagaat ctggtaaagg agctgcactg
     4621 agtatggatt ggatgaattt gctcggtcag ctgactgcgg catccagtgt gtccggcagc
     4681 gaagaaaata tcgctgatac ggtgctgtct ctgctctctc catattgtga cagcgcagaa
     4741 cagcggcagg ggaacgtcct cggcacactg ggagagcgtc agcccggaaa gccccacgtg
     4801 ctgctggatg cgcacatgga tcaggttggc tttctggtaa ctgccatcac agaagaggga
     4861 tttctccggg tgggaaatgc cggcggtctg gactttcggc tcatgccggc gcagcgt
//
LOCUS       DAWENO010000157         4845 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6527_length_4845_cov_9.118998, whole genome shotgun sequence.
ACCESSION   DAWENO010000157 DAWENO010000000
VERSION     DAWENO010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6527_length_4845_cov_9.118998"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1053 a         1364 c         1255 g         1173 t
ORIGIN      
        1 ccgtggtcga tgaacagacc ctttccgatc tgcgcaccgg gatggatctc gatgccggtc
       61 ttgtgtcttg cacgctggga tatgatgcgt gcaatggtat agaatttgtg ctgatagaac
      121 cagtgggcac gccgatagct gcgcactgct ttcagcccgg agtatgtcag cagaatttca
      181 aagctgctgc gtgccgccgg gtctttttcc ttgacgatgg cgatatcttc ccgtagctgt
      241 cgaaacatat tccaatgtca cctcccaaaa ttgtccatgc acctgcctgc gacagaaaaa
      301 ccgcctctgc aagtgctgtt ttcgtacact tccagaggcg gaaaaaattc ttccacggtt
      361 ccactctgat ttctcactca acaggacacc ccaaaacggg aatcccttct gccgataacg
      421 gagcaaaacc cggacacgga tactccggcg tcaaacgcca ttccccatgc caacggagaa
      481 cgccgtctga cgctctcatc gcagttgcac ttcaccccgg cgcagggcat tgttccaccg
      541 acccaatgct ctctgtacgc tgccgccgga gatactctca ctgctgcgtt gttcgattca
      601 tctttctgta atcctctaca gtactattat tatatgcgat ttcggcggtt ttgtcaatga
      661 attttttgtg gcgcagaatg attttctccc tgcggcttat ttttccagca gttcgctcca
      721 ggtctgccgg attcctgcgc cgtggagcgc cacgtagtac atcagataaa aaatgtcgga
      781 agcatccacc gcaccgtcac agtcgatgtc tgcggcagtg atctgctgcg gcgtcagttc
      841 gccggcattg cccacggcga tgcttgccac atagtacatg gcgtagaaga tatccgtgga
      901 atccagcgtc ccgttttcgt ccacatcgcc cagcagcgtg tccgtgctgt tcagatccgc
      961 aaagggaatg ctgtagttct cagcgtattt ctgtgcgaaa gagccggcat atccccggat
     1021 ggtcagcccg tcacattcac tgaatacaga ttgccccatg ctggtcacgc tgtcgggaat
     1081 ggtgatacgg gtcagattgg tgcaggatgc aaacgcccag ttctccattt tggtcagact
     1141 gtccggcagg cgcagacccg tcagcccagt acacgatgaa aaagcattgc tccggatggt
     1201 ggtcagacct tcgggcagga cgatctccgg cagactgtcg cactgataga atgcggcaac
     1261 ttcaatggtg gtgatatttt tcgacagctt gacacgacgc agtcccttac agccggaaaa
     1321 ggcagaagag ccgattctgg tgacgctgtc gggaatggtc acgctggtca tctgtgtgga
     1381 acaggcggtc acgagaccaa agtcggagga aaaggcatag ttggcgatat cggtcacccc
     1441 gtcggggatg acaacatctc cggtacaggt ctgtccgtca acaacaattt ggttgacaat
     1501 caccagcgga ttttctgcct gccgggcttt cagccacggc gtgaacgaaa acgcctcttt
     1561 cccgatatag gtgacactct ccggaatggt gatatcagtc agttttgaac agccccgaaa
     1621 gacagcatct ttgatactgg tcaccggaca gccgtcgatc tccgccggga tctccacagc
     1681 agtcaccgca gtgtcacagt cggtaatggt gaccgttcca ctgctgctga cagaataagt
     1741 cagactttca taagtgccgg tggctgcgct cgccgacaat accggctgca cattttgcag
     1801 cacacccgaa aagccggaca ttcccgcaca gcccagacac accgcaccgg cagcaatcgc
     1861 cgccgcaagc tttttccaca atttcattga catttcccca ttcccctttt gcaaaatatg
     1921 attactttat tatagcatga ccttgtgaaa aagtcaattg gattcgcttt tccccaccaa
     1981 aaaaccggtt cctgtaccaa cagaaaccgg ttcttatctc tcacagtgtg cgcacacaca
     2041 ccaaacacgc ttatgcggaa acgctctgac tgagcgcctg ccgttttttc gccgcagcgg
     2101 tgcgccatga aatcaatccg actgccaatg ctgccggaat cacgatcagc aatgtcacag
     2161 acaggaagtt caccggaatc cagcccaggg cataatcgcc gccgaatgtg gaaaagctgt
     2221 tgacggcaaa gtgcatgatc atagaaaaaa ccacggactt atagtgatgc gctacccagc
     2281 ccagcaccag gccgccgaaa aaggcataca cgccctgcac ccagttcatg tgcgccacgc
     2341 cgaacaggaa cgcctgcagc aggttcgccg cccagaatcg accgaacgcc ttttctgcat
     2401 acttcatggt cagcccacgg aagatcagtt cctcgccgat gggcgccagg atcaccgttg
     2461 caaagatcac catgtagttt gtgccgatgc cggactgctc cagcatcgca taggtatctt
     2521 ccaccatacg agggaacagc agggtctcca gaccggctgt tccgctgctg aatacagcca
     2581 tcagaatgcc cagtcccagc cccatcagaa tcggttccgg tctcagttcc tgcacggtct
     2641 cccgtggggc tggccgtggt ttctgaaagc tgagacgata ccagaacgca aaccccactg
     2701 ccgacaccag atgcgccagc gccgtggcaa cggggatgct ggcggagacg atttcctcat
     2761 acgccgccat aacgttctgg gttctgccgc tctggaacgc attcacaaat accatgatga
     2821 caaccaccac aacggaaacc agtatctgca cggcaaaaaa gcctaccagc ggcagcagcg
     2881 acaggaaaaa gtttgccaca cgtcccggct cttttttctg cggcggctga tacgcatagc
     2941 cgttctgcat ttgcggcgca tacggctgat atggctgctg gtactgctgt tgaggatagc
     3001 cgtagttgtt ctgcggttgc tggtactgct gctggggata ggcatagtta ttctgcggct
     3061 gttggtattg ctgctgggga tagccatagt tgttctgcgg ctgctggtac tgctgctggg
     3121 aatagccgta gttgttctgc ggttgctggt actgctgctg cggatagccg tagccattct
     3181 gcggctgttg gtattgctgc tgcggattgc cgtaattatt ctgcggcggc ggtgtctgat
     3241 tcgggtactg ctggggattc tgctgttctt ccatagggct ttcctttcac tcagtcacgc
     3301 cggaaaatgt ctctggtgta gaccttttcc cggatgtcgt ccagacagct gtcatagcgg
     3361 tttgcgatca cggcatcgca cattttctta aactgttcca ggtcattgac cacagtgctg
     3421 ccgaaaaatg tagtgccgtt ttccagcgtc ggctcataga tcaccacgga ggcacccttt
     3481 gccttgatgc gtttcattac gccctggatg ctggactggc ggaagttgtc gctgttcgcc
     3541 ttcatggtca gacgatagac accgactacc accggctttt ccttctggct gtcgtaggtg
     3601 ttgtcatcgt cataatcata gcagccagcc atgtgcagca cacggtcggc aatgaagtcc
     3661 tttcgggtgc tgtttgcggc aacgatcgcc tcgatcagat tctccggcac atcagcatag
     3721 ttcgccagga gctgtttcgt atccttgggc aggcaatagc cgccgtagcc aaaggacgga
     3781 ttgttatagt gtgcgccgat acggggatcc aggcaaacgc cgtcgatgat ctgccgggta
     3841 tccagccctt tcatttcggc ataggtgtcc agttcgttga agtagctgac acgcagcgcc
     3901 aggtaggtgt tggcgaacag tttcactgcc tctgcctcgg tgtagcccat aaacagcgtg
     3961 gggatctctt ccttgatcgc accctcccgg agcagtccgg caaaggtatg tgcggcggcg
     4021 gtcagtgtct cgtctgcggc atccgttccc acaatgatgc gggacggata gaggttgtcg
     4081 tacagcgctc tgccctcccg gagaaattcc gggctgaaca ggatgttctg acagtggtac
     4141 ttttcccgga cggactgggt atagcccacc ggaacggtag acttgatgac catgactgca
     4201 tccggattgc aggacagcac cagttcgatc actgcctcta ccgcagaggt atcgaaaaag
     4261 tttttgcggc tgtcatagtt ggtgggtgcg gcgatgataa cgaaatccgc ctggcggtat
     4321 gctgccgctc cgtccagcgt tgcagtcaga tccaggtctt tttctgccag atactgctgg
     4381 atctccgcat caatgatggg cgatttccgc tggttgatga gttccacctt ttccggaatg
     4441 atgtctactg ccagcacctt gtgatgctgt gccagcagga tggcgttgga aagtcccaca
     4501 tagccggttc ctgccactgc aattgtatat tttttctcca tgtccttctt cctcatatac
     4561 aagcctggtt ttgcatcctt tccacgtgtc atgtccggac ggcacaggct ctctaaaaca
     4621 atgaaaaacc ggcgacaccc tgccgccggt tttctgcgtg tgtatcttat atgctattgt
     4681 agcacatttt ggcgtatccg tcaagccggc ggataactgc ctttacacaa atcaattttc
     4741 aagtcctcct tgaaaagggg gatgtcagcg taagctgaca gggggattaa cttctcattc
     4801 aaacctatgg cttgccagct cagtaccagt cgcacatttc agaca
//
LOCUS       DAWENO010000158         4819 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6569_length_4819_cov_8.568010, whole genome shotgun sequence.
ACCESSION   DAWENO010000158 DAWENO010000000
VERSION     DAWENO010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_4819_cov_8.568010"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3614..3623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1128 a         1277 c         1105 g         1299 t
ORIGIN      
        1 atcgctttcc tgtgtctccg gacgattggc agagggtgtg atttggaggt tggtttttgc
       61 cacctgcatg gttgcctcag aggaagtctt ggatgcctcg ttgatgctgc caatgacttc
      121 gaagtgcgcc gatgctttca aatcccctgt ccagtccgta gaaccataca gtttcagatt
      181 catctccttg agattgtcca gatctgcatt tccgtagatg cgggagaaga tgatgcgcac
      241 actccgcagg cgctgaatgg tatccggaac ggtatatgtg acattgccgt ccgcatctac
      301 cgttagggca tcagaattga ccgtcagact aggcacaata tttgcctcgt tgtctgcggt
      361 atatgtcgta ttattccaat caaaaagttc aattctatcg atctctgccg cccgtcctgt
      421 accgatgccg tccagaacga tcttccgggt atcaaagccc tccacatcct ggagacagtc
      481 gataggctca ttgccgacgc tgtccatatt gacttgcagc agcgcatgag agctggtaga
      541 acgggagttg ttggtcagca ggaactgata ccaggtctgt tccgttttgc tccggttcgg
      601 cacagccagt gtcggataaa cgggatgagc gtttccgttt ttgtcatagc tgacagactg
      661 ttccttgtac gcttctgtgc cgtccttgcg aatcgcatga acgctcgctt tcagttccgg
      721 cagcaccttg gacacgttca gtgtgacggt atcttccacc gtctgatcca gggcagtctg
      781 gtcgtgttcg ccaaagctat aatcggtttt cagcacgccg tgaggtgtga tatccttttc
      841 tgtggtcggc gtgccgtgaa tctgcacata gcagttcccg tcatcgttgg catccgtcag
      901 cggaatattg tcgctgatgc gctggaagta cacggtcata ccagtgagat aggtcagctt
      961 accgctccac gcagacttcg gaatttccag gtctccgttt gcatttacgc cgtagttttc
     1021 cagcttgttc agtgtgacat cctgatctgt cagcgtcccc cggttgttca ccttgccgtg
     1081 cagcacgatt ttctcgatcg tcgccttttc cagcaagctc ctgctgagct ggatgttgga
     1141 cgcaaccagt ccgccggcag cgcaatcctc gtccggatac agaccagtga tatccagtgt
     1201 tgccgggctg atctcagaaa tgctgtcgtt ggaaatgaca aagcgagccg ccgccgtatc
     1261 atcgttcaga gaaactgtct gcggattgcc tttctgtatc agatcgccga tggagtcatc
     1321 ataaacgcct acgccggtca gcgccggttt tgcccgctcc gttttgataa ctgcattctt
     1381 ttcatcaata tctttcgact ccgaaaccca cttgccttcg ccgtcttccg acgctgtcgg
     1441 cggctggtaa tagtgaatct cataagaggt gtcgatcttg ttgtcatatg tccacaagtg
     1501 cggaaatgca ccacgcatgg tgataagacc gttgaagttg gtatagcgtg ggatctcctc
     1561 ccggaacaca aacttgacct cattgcagaa tgtcagacca gcctgtgtgg tcagtgcgtc
     1621 gccaatggca agcgtccaga tcttttcgtc tgctgcgcta acattggcat cctgtttcag
     1681 acccaagccc aattcgctgg cagttttata gatcacatca ccgttttcat cggtaaagcc
     1741 aaactggata aactgattga ggtctgttcc atccacctta aaccagtctt tcagctgaat
     1801 gtccggataa tgcaccgtgt cgttctgcaa attgatctgg atttcctgca ggtcaaaatc
     1861 ttccggcacg tggtctgtcg caacggcatt gaacaccggg atattgccga tgctgttgaa
     1921 cccatccagg taatattcat ccaccgcacc gatgccaacc gtcttatctg ttacttcttt
     1981 gccgtctgcg tccttgacat atttgtgatg cgcaaagtta tcccaggtat ggggcgtgaa
     2041 ctctgtagtg tccgaacctt gtttcgacca gtgaatgctg ctgccgccgc cgaacgaaat
     2101 tgttgttgtc agtccagaag tgtttttgaa tccggtgttg ctgttaaagt tgttcggata
     2161 cggaatcgca accatgtatt tccgggcagc atagtacttt ccgttggggt caccctggtt
     2221 cagttcctcc gctttctcct tggaatatag catatgggat ttgtcggtag cttctctttg
     2281 cagaatgatc gtacctgtac cggaataacc atacggtatg gtttcgccgg gaatattggt
     2341 gaaattctcc agatcccact ccgttttcca gtccggtgta tcggtgacat catagatcag
     2401 aacaccgccc tcattgggct taccgacaaa gtcatgatgc tgataaaaat cagcggaaat
     2461 atttgtgttt ggcgtgttcg tatcatcctc gcaccagcga agaatatcct catccaggat
     2521 actccgcatt ttgtccgggt tatactgcgc ctgtaatgta atcaaatagt tgtcgatggt
     2581 gctttctttt tctgtagagt cgttctgcac tgtgacttcg tataccatgt agttatactt
     2641 ctgccacatg accgcagtac ttttatcagt acttgcactg tccagctgca ctgtcggaac
     2701 gcttttgatg cttgtactcc actccaggtt agaattgaca aaagtaactg tacgtgcctg
     2761 tttttcctgc tcagaatcca gatcatctgt agaccagctg ccgcttaatt cattgccgct
     2821 gttgtcataa tacttgtcat atgaaaactt tacgttcagc gttgcagctg tattttccgg
     2881 tatcgttact ttgtcacttg ctgtataaaa ccggatatcg aattgcggcg tgcaatcctg
     2941 ggaaagtgat tcataaacat ttttcagttc tatttttcca cgagcatatt gactgctgtc
     3001 aattctatca ccaatctcat tatagatatc agctgaatta ccaaatacac tcatattatt
     3061 cagctttgcc tctatgccga tatattctcc gttttctttt tgcggactgg gaatcttgtc
     3121 aaacgaattc acttgaacca gtacattttt ttcatcccaa tacaaatacg gcagtgtgat
     3181 ctccagatcc tgccccttga cgtaacctgt ttgaaaatca ggtctgatat tgacagtcat
     3241 caatgtctgc tgctgcttgg taatcatgta gaaactgccg acaccgtcat tggtctgtgc
     3301 cgtcagtgta gcaccgcctg acagcactgt tccaaaccca acttgaaaac cgctgggatt
     3361 tcccaacgcc tctgctgcga tgaagatcgt cccccagtcg cacgaagtca gcaccatcac
     3421 tgttgctaat ataccataaa tgattctctt ccatatgctt atttgtcgat acattacaat
     3481 tccccctgcc atacggcaca tctgtttcgt gaattaacaa tttcaataaa catttccgtt
     3541 tttttcaagc atcttgatga aaaagcgttt ttcttggatt tcagcagttt tattcgctga
     3601 aatcactagt tttnnnnnnn nnnaatccca cattttatgg caaaaaacat ccaatttcga
     3661 ggaaataaaa acgagatatg tccgcaggga aagtgccttt gtttgccgta cagatacaaa
     3721 aaagctgtag aactgatacg tcctacagct ttgaaaaaat gccttatttc attatgcttt
     3781 tttcgtctct ctgtccgaca ggaacaggct caccagcagt agcaccggaa agacgatcgc
     3841 cgccaggatg ccgatgtgca ggtcattctg aaacgcactg gacaccagcc cggcaagtgt
     3901 gggaccgccg gagcagccca catcccctgc cagtgccagc atggcaaaca tcgccgtgcc
     3961 gccgcctttg atggcagcgg atgctttgct aaacgttccc ggccagagga ttcccacaga
     4021 aaagccgcag accgcacagc cgagcagtcc cacgaacggc acgggaacaa aaacgatgca
     4081 caaatacgac gccatgcaca gcacacagct gtacttcata aaacggtcca ggttcagccg
     4141 atcgccgaac ttgccgtaga tcagtcttgc cgttcccatc agcagggcga acgacatcgg
     4201 accggcaagg tcgcccacgg ttttggtgac gcccagaccc tgctctgcaa acgtagatgc
     4261 ccactggctc actgcctgct ccgatgcgcc ggcacagagc atcatcagca gaaagatcca
     4321 aaaaatcttc cgccggaaca atgcccccag agacatccct ttttcgccct cttcgtggag
     4381 cgaatacaac ggaacagttg caaagaaaat cgtattgcaa accgggatca gtacccacag
     4441 caaggtgaga atcttccagt tttccacgcc aaacagggcg aaaaacgccg tagacagcag
     4501 taccacgccc atatgtcccc agcagtaaaa cgaatgcagc aggctcatcg ccttttcctt
     4561 gttttctgtg ggacacgcct ccagaatcgg gctgatgagg acttcgatca gaccgccgcc
     4621 gatggcatag actacgaccg acagcacgat gccggcatag gcgttcggca gaagatccgg
     4681 gagaaatgtc agcccaagca agccggctgc cgcaaaaata tgcgccatga tgaccgatgc
     4741 ccgatagccg atcttgtcca cgaacccggc agaaagcatc tccaccagca gctggacgca
     4801 gtagtttatg gtaatcaat
//
LOCUS       DAWENO010000159         4778 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6621_length_4778_cov_10.126191, whole genome shotgun sequence.
ACCESSION   DAWENO010000159 DAWENO010000000
VERSION     DAWENO010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6621_length_4778_cov_10.126191"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3617..3626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1100 a         1226 c         1042 g         1400 t
ORIGIN      
        1 gtaatccgca gtggcgatca tctgcaccac cagttcccca tctgacaagc ccaattttgc
       61 cgggacatag tcacccagca cgctcgcctt ggactctccg aattcgcttg ctctggtttt
      121 ataaacctgc tgctggttgg tgaaaaagag cagttcgata cggtttgttg tttccagatg
      181 cgctacgatc ttatcttcct ctttcagctt ttgttcactg ctcatgcgga gcgatgccgg
      241 tgtgatcttt ttgaaatagc cgctctccga caggaacaaa tgcaccggat aatccgggat
      301 atcctcttcg tccgactctt cctctacatc ggatgcgtag tagaacagcg tctttcgggg
      361 ctgaccgtat tttttcgccg tttctttcag ctcagcgatg atgattttct tcatcttttt
      421 cggatcggca atggtagatt ccagctctgc gatatcctgt ttcaactggt cgatctcctg
      481 cgtccgtttc agaatatatt cccggttcag atgacgcagc ttgatctccg ccacatactc
      541 cgcctggatc tcatcaatgc caaagccgat catcagattc ggcacaacat ccacttcttc
      601 ttctgtctct ctgacaatgc ggattgcctt gtcgatgtcc agcagaatct tgtccagacc
      661 cagcagcaga tgcagctttt cctgtttctt tttcagatca aacaccagtc ggcggcgcac
      721 gcattccaga cgaaaggcag accattcttc cagaatttca cgcacgccca ttaccttcgg
      781 cgcaccgcct accagaatat taaaattgca ggaataatta tcctggagcg gtgtcatgcg
      841 gaacagtttc tgcataactt tttccgggtc cgagcctcgt ttcagatcta tggcgatctt
      901 caagccgtca atatcagttt catcccggac ataggaaacc tcacgcagct ttccctgctt
      961 gatgcagtct acgatcttat ccttgattgc ctcgatcgtg gtgctgtacg gaatctccgt
     1021 gatttccagg cagttgtccg ccttgttata cgtccacttg gaacgaacgg tcacgctgcc
     1081 acgtccggtt tcgtaaatct tctgcaattc cgattcgtta taatacagaa atccgccgcc
     1141 gggaaaatcc ggtcctttta aggtggaaag gatgtcgtgt tccggatttt tcagcactgc
     1201 aatcgctgtc tcgcagactt ctgccagatt aaacgggcag acactgcttg ccatggatac
     1261 tgcaatacct acgttggcat tgaccagaac cgtgggaaat gttgccggaa acaaggtggg
     1321 ttcctgcatg gtattgtcat agttgggaac aaaatccacc gtatccttat caatatcccg
     1381 gaacagctcg ttacagatcg gttccagctt tacttctgta taacgggatg cggcatatgc
     1441 catatcacgg gaatacgcct taccgaagtt acccttggac tgcacatacg gatgcagcag
     1501 cgcctcattg ccccgtgcca gacgtaccat ggtctcatag atcgcactgt cgccgtgcgg
     1561 gttcagacgc atggtctgcc cgacaacatt tgccgacttg gtcagattgc ctgtgaggag
     1621 tcccatcttg tacatggtat acagcagctt tcggtgtgac ggcttgaaac catcaatttc
     1681 cggcagcgca cgggaaatga taacgctcat ggcatacggc atatagtttt tttccagcgt
     1741 gtccgtgatc ttctcttctt ccacaacgcc ggcaccggca atataagcgt ttggtatttc
     1801 tgtttgtttc tttcgtgcca tttttgtcct cgcttttatg agatatctgc cagttccaga
     1861 taattgctgc cgaattcagc aatgaaatct tttcgtccct ggaggtcatc ccccagcagc
     1921 atatcaaaca ttgcagctgt ttcttcgatt cctgccgctg taacttgaat cagacggcgt
     1981 gtctccgggt tcatggtggt cagtgacatc atatccgcct cgttctcgcc cagacctttt
     2041 gaacgctgca aggtgtattt ttcgttttcc agagtcgcaa ggatctctgt cttttccttt
     2101 tccgtatacg caaaataagt gcgctttttc gttgtaattt cgaatagcgg cgactctgca
     2161 atatacacgc agcccttgtc gatcagcgtc ggtgtcagac ggtacagcat tgtcagaatc
     2221 agtgtccgga tctggaatcc gtctacgtcc gcatcggtac agataacgat tttattccag
     2281 cgcaggccat ccaggttgaa agaggaaatg tccttatttg ccttggtcgt tacttcaacg
     2341 ccgcagccca gaagtttaat gatatctgtg ataatctcat ttttgaagat cttattatag
     2401 tccgctttca gacagttcag aatcttacca cgcaccggga tgaccgcctg gaattctgcg
     2461 tcacgtgcca tttttaccga gccgagcgca gaatcgccct ccacaatata gagttcccga
     2521 cgggacaggt ctttactgcg gcaatccaca aacttcggga tcatgttggt cacatcaatg
     2581 ctgccggtca gctttttctt catgttcaga cgtgtttttt ctgcattttc acggctgcgc
     2641 ttgttaatca gaacctgcgc tgcaattttt tctgcctcgt ccggattttc tataaagtaa
     2701 atttccagct ggtgcttgag gaagtcggtc attgcctcat agataaactt attggttatc
     2761 gcctttttcg tctggttctc atagcttgtc tctgtggaaa agctggaaga aatcaaaacc
     2821 agactggatt caacgtcctc aaatttcagc ttggactcat ttatcagata cttgttgttc
     2881 tgccgcagat aggtgtcgat ctgcgagacg aacgcctgtt ttaccgcctt atccggagaa
     2941 ccgccgtgtt ccagccagct ggaattgtga tagtattcca gacgctgtac ctggttggaa
     3001 aatgtcagtg ccacgcccag ctttacttta taagtaggct tatcttcacg gtcacggccc
     3061 tctcgctggc aactccagtt ctgaatggaa gtcagcgtat tttccccggc gatctctttt
     3121 acatagtcag cgatgccgtt ttcatagagg tactgctttt ctgtaaaggt gccatcctct
     3181 tcttctttcc ggtagagaaa cagcagattc gggtttacaa ctgcctgtct gcggagcgtg
     3241 tcacaaaaat agtcgtcgga aatttcgatc tctgtaaata cgtccaggtc gggcagccag
     3301 tgtgtccgtg ttccggtttg ttttttcttt gtcggttttt tctgcaaacc gcctacattg
     3361 acgccttttt caaagtgcag attatactga taaccgtcac gaaaaatttc tacgtccata
     3421 taggcagacg caaactgtgt tgcacacaat cccagaccgt tcaagcccag tgaataagca
     3481 tagttatcat cgttggagtc atatttaccg ccggcataca gttcgcagta caccagttcc
     3541 cagttatacc ggttttcatt tttattaaag tctaccggac agcctcgtcc gaagtcctcg
     3601 acctcaatgg aattatnnnn nnnnnnaatt atcccggtat cgtgtcacga tgatcttgct
     3661 gccatagccg gcacgagcct catcaatgga gttggagatc acttcaaaaa tagagtgcgc
     3721 acagccatct ggtccgtctg aaccaaaaat aacgcccgga cgctttcgca ccttatccgg
     3781 gcctttgagc atggtaatgc tttcgttgtc ataggattgt ttttttgcca tatcgtatcc
     3841 tttcctccgg aaaagtcatc cggaattttg ctgcattgtt cctatcatac cacaaagcgg
     3901 cgaaaaaatc aagccttttc cggaaaattg cgattattcc acgctccgga acaagcccag
     3961 tcgatacgat acagaacaaa aatcttgcaa aatggcattt gcagaattcc ctccatgctg
     4021 gtcaaaacat caatttactt tgtttatttt tgtagaatca tccgtacagc aagtgattgt
     4081 gccgattttt caaaagaaaa taaacttgac attttaggag gactgtgcta taatatataa
     4141 gtcggttgac gcagaacgga taaaaccgac agataccttg cgggtgtagt tcaatggtag
     4201 aattccagcc ttccaagctg gttacgtggg ttcgattccc atcacccgct ccaaatgcgc
     4261 ctttaactca gctggataga gtaactgcct tctaagcagt aagccactgg ttcgaatcca
     4321 gtaaggcgcg ccaaatgtcc cgctgaggga cgcaaatatg gtgggtgtag cttagctggt
     4381 taaagcgtcg gattgtggtc ccgaagatcg tgggttcgaa tcccatctcc caccccatca
     4441 aaagccttgt tcatctgaac aaggcttttt cttttatttc cgtatacata cataaggata
     4501 cgcatacaaa aagccggtct ctgcaattcc gcagaaaccg gcttttcttg atatgatttt
     4561 agagttttgc tttacgccgc caaaacgctg tccattccct tgatggtgaa tgtaacgcta
     4621 aagctatcct tcggcatcgt gtagttcagc gctgccagat cggtgttata gatgttcacc
     4681 agttcgatgt acatatttcc ctggtcgtct gccattacca gcggattgtc gatggcgatt
     4741 tcctggtcat cgcactttac aacaacatcg ctgacctt
//
LOCUS       DAWENO010000160         4769 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6631_length_4769_cov_9.385872, whole genome shotgun sequence.
ACCESSION   DAWENO010000160 DAWENO010000000
VERSION     DAWENO010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_4769_cov_9.385872"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    227..236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3301..3310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1355 a          980 c         1206 g         1208 t
ORIGIN      
        1 ttcttttccg tcaacagtga ccggctgtgc aatgaatcga tccagatcgg aatttaccgg
       61 gatatctttc gggacaagaa ttgcttttcc catgtccact tttacgcttt cgatcttgtt
      121 gtcagcaccg ggatacagtg tcagatactt gatgccggaa ttggtttcca gcgatatctc
      181 ggttttatcg gtcatgccca tatcatagac gtacttgccg atacatnnnn nnnnnnctat
      241 tataccgaaa aaccggaaaa aaatcaagca tatccggaaa aaattttcaa attcggtttt
      301 tttacgcttt ccgggcagaa aagccttgca aagcaaaaga aattgtgcta taataaaaaa
      361 tgtgtaaatt tacgcaggtt ctgtgaagtt gtatttcaca gcgttcctgt aatttcagca
      421 atagaaatga gggtcaagat tggatttaca gatgattgcc tatttggcag aactgggaaa
      481 acttcatttt acagaagatg aaatgaagca gatggcatct gaaatgacaa gcatcattga
      541 aatcatggac actgtaaagg aaattgatat tcagtatgat gcgtatgccg acaataaaaa
      601 tgtttatcta aacgatttgc gagaggacat caaggcggat agcttcccga cagaaaagat
      661 tctgcaaaat gctgtgaatg acgagaattg ctttgtcgtt ccgaaggttg tagaataatt
      721 actataaaga aaaggaggca ttcgtctgcg ggcgaatgag taaaacatat ggatcttacg
      781 caacaaacta tcggaaaact gcgtcagatg ctggacagaa aagaagtttc tgcgccggaa
      841 ctgacaaagg cttatctgga tcggatagca gcttatgata aaacgattca gagttatatt
      901 actgtaacag cagaacgtgc catggcagat gcagatgctg cgcagaaaaa gattgatgcc
      961 ggaaatgcag gcgtactttg cggcatccct atggcaatca aagacaacat ttgtacagac
     1021 ggcattaaaa ccacctgtgc ctcgaagatg ctggaaaact ttatcccgcc gtataatgca
     1081 acggttatgg aaaagctgat ggcacagaat gtggttatgc tcggcaaggc aagcatggat
     1141 gaatttgcaa tgggcggttc tacacagacc tctgcatttg caaagacacg caatccgttt
     1201 aacacagaat gtgtaccggg cggttcttcc ggcggttctg cggcgagcgt atctgcatct
     1261 ctggctgcgg ctgcccttgg ctctgatacc ggcggttcga ttcgtcagcc ggctgcattt
     1321 tgcggtgtga ctggtctgaa accgacatac ggcagagttt cccgttatgg tctggtcgca
     1381 tttgcatctt ccctggatca gatcggaccg attgccaaga atgcacagga ttgtgcatgg
     1441 attttgaatg cggttgcagg attcgatccg catgacggaa cttcatccaa gcgtccggta
     1501 gaagactata cagcaaagct gggaaaagat atcaagggac tgaaaattgc gatcccgaag
     1561 gaattctata cagagggtat cgacgaagat gtgcggaacg cagtaatgca ggcagcaaag
     1621 acctatgaaa agatgggcgc tgaactggta gaatgcagta tgcccagctt gaaatatgca
     1681 gttgctgcat attatctgat tgcatccgca gaggcgtctt cgaacctgtc aagatatgac
     1741 ggcatcaagt acggtcaccg tagcaaggtc ggcgacacct tccaggaact gatctgcaac
     1801 tcccgcagtg agggattcgg cagcgaagta aagcgccgta ttcttctggg caactatgcg
     1861 ctttccagcg gttactacga tgcttattat ggaaaggcaa tggcactgaa acagcgcatt
     1921 tccgcagaat atgcacacat ttttgaaacc tgcgatgtga ttctgacacc gacaacaccg
     1981 tctgttgctt atggtgtaca tgagaatgtt tccgaccctg tcaaaatgta tcaggcagat
     2041 atctgcaccg ttactgtcaa catcgcttcc ctgccggcta tttctacacc gtgcggctac
     2101 aatgcagacg gtatgccgat cggtatgtcg attgtcggaa agcagtttga cgaggcaacg
     2161 attttgcagg tcgctgatgc gttcgagcag cagttccaga ctgctgtccc ggtattgaaa
     2221 taaggaggtc aaagttacat catgtcatca tatattcctg ttattggtct tgagatccat
     2281 gcagaacttt tgacaaagtc taaggttttc tgtacttgca gcgcagaatt tggtggaagc
     2341 cagaattccc gctgctgccc ggtatgcacg ggtatgcccg gaaccctgcc ggttatcaat
     2401 cagacagcgg ttgaatacgc tgtaaaagca ggtatggcgc ttggctgcga tatcagcaag
     2461 ttttctgtat ttgaccgaaa gaattatttc tatccggact tgccgaaagc atatcagatc
     2521 tctcagctgt actatccgct gtgcttagag ggtaaggttg cgattgatac accggatgga
     2581 cagaaataca ttcgcatcaa ccatattcac ctggaggaag atgctggtaa gctggtgcac
     2641 gatgatttca atgcagtttc tctggcagac tataaccgct gcggtattcc cctggtggaa
     2701 attgtaacgg agccggacat ctcttctgcg gaagaggcaa aggcatttat tgaaaaagtt
     2761 atgctgctgc tgcagtatgc aggtgtcagc gactgtaaaa tggaggaagg ttcacttcgc
     2821 tgtgatgtca atgtgtctat catgcgtcct ggcgataagg aactgggaac acgtgctgag
     2881 atcaaaaaca tgaactctct gaagtccatc acccgtgcga tcaactatga gatcaagcgt
     2941 cagtccagac tgctggatgc cgggaaaaag gttgttcagg aaacacgccg ttttaatgaa
     3001 aacaagggtg agacttcttc tatgagaagt aaggaaaatg ctcacgatta ccgctatttc
     3061 ccggagccgg atatcctgca ggtaaacttt acagacgaaa tgctggattc cattaaaaat
     3121 atgctgccgg aactgccgta taaacggatg gagcggtata tgaaggatta cggtctgtcc
     3181 aaaacagatg cacagattct gattaaccag aagagcgttt ctgatttcta tgatgcggct
     3241 gtttctgctt ataatgcgcc gaagagcatt gcaaacttta ttatcgtgga attgctgcgc
     3301 nnnnnnnnnn gggcgaggtt tctatgacag cactccccat ctcggcagcg gaatttgcag
     3361 aactggtgaa aatgtcggat acagagcagg tatcgaagaa cgatgcaaag aagatcctcc
     3421 gtcaaatgat cgaaaccgga aaggatgcaa aaacaattgc agaagaatcc ggtatgctta
     3481 ttgtaaacga taccaagaaa gcaaaagaag tcatcggaaa gattctggca gataatacag
     3541 aggcagttgc ccagtatcag agcggcgaga aaaaggtatt cggattcttg atgggtcagt
     3601 gtactaagtc cctgcgtggt gtctgcacac cgggtgcaat taaggaattg ctggagacga
     3661 aactggctga ggcaaagccg tctgctgacg aagaaaccaa aaatgcgaaa accgatgcag
     3721 cagaagaaac tcgtgaagta gactgcacac cgttctctaa cccgaatcag tatgttccgg
     3781 gaagaaaaga cggtgtaaca cagatcagta cagatcacct tctgcatgag tttgattttt
     3841 ctgatgcggc agaccatgtc ggagaagaaa tttcgctccg tgcgtgtgtg cataaaattc
     3901 gtcagatgag tggatttgcg tttatcattc tgcgtactgg acgctatctg atccagtcgc
     3961 tgtatgttcc ggaacagtgc aaggattcta tcacaggact gcgtgagggc aactttgtct
     4021 gggtacgtgg taaggtgaca aagaatgaaa aggcagtcgg cggcgtagaa cttcagttgt
     4081 ccgaactgga aatgattgca aactgcaatg aggaatatcc gctgaaggtt tccaacaaga
     4141 aactgggctg tgcgctggat atcaatctgg ataaccgttc tgtagcactg cgtaacatct
     4201 atgagcgtgc gattttcaag atccaggaag gcgttgtcgg cggtttccgg gagttcttga
     4261 ttggacaggg ctttacagaa attcatacgc cgaaaattgt tgcacagggc gcagaaggcg
     4321 gcgcaaacat tttccatctg gaatattttg ataagcctgc gttcttaaac cagtctccgc
     4381 agttctataa gcagactgct gtggcattct tcgaccgtgt ttttgagatt gctccggtat
     4441 atcgtgcaga aaagcacgca acttcccgtc atatcaacga gtacatcggt ctggactttg
     4501 aaatgggtta tatcgacagt atgtacgatg taatggcaat ggaaactgcc tgcctgcgtt
     4561 atgtgatgga atatctgaaa aagcattatg cgtatgaact ggaacttctg gaagctgatg
     4621 ttccggtgat taaggaaatt ccgtgtgtca ccttgctgga gactaaggaa attcttggaa
     4681 ataaaggctc taagaacaag cttgacctgg aaccggaaga tgaagtggca atttgcgaat
     4741 atgccaaaaa gacatttgac agtgacttt
//
LOCUS       DAWENO010000161         4712 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6722_length_4712_cov_10.007730, whole genome shotgun sequence.
ACCESSION   DAWENO010000161 DAWENO010000000
VERSION     DAWENO010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6722_length_4712_cov_10.007730"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1270 a          924 c         1245 g         1273 t
ORIGIN      
        1 aaaaaagtgg aagagcgttc ctggcatgat agctatgaaa gggtaaatga catcatcggc
       61 ttattcgaat tatgtgttgt tgtcatttca actatagttt ttatcatcgc aaaagttaaa
      121 aacaatgaga agataataag aaaacaaatg ttattatggt tcgtgtttgg aatcagcacg
      181 atcgcactgt tatgcagcaa tattgttgcg accggtttat cggatgaaat ggattatcgt
      241 ccaaaatact atgcgttttc tgataatcag catacacttg ttttggagga aaggtcgttt
      301 ttattgtatg gaggcggata tatctttcag attcaagacg ataacagtgc ggttctatta
      361 gattgtttta caacagatga cggcggcagg aacaatggaa tgtacgatat tgtttggcat
      421 cagaattatg ctgaaataac ttatcgtaca tttaccactg aaaatgataa aaaaacaatc
      481 gttgtcaatt tcaagtgatt tctgtttttg aaaggatgga tgcagctatg ttgaagtatg
      541 ggatttatgc aaaagcagta gagcttttgt gctttcagcc ggcactcgaa aagctttccg
      601 ctgtatatcc tgagatggat ataaaaagct acaagaaaaa cgtaaaacac gaatacaaaa
      661 atatgctttt gcgaacgccg gacataggag gcagttccct ggagatgaat ctgtatatcg
      721 cagcatttgt cttttctctg cacaaagcag aaccggacaa gatcacgcct gcggttgtgg
      781 atgcaatggt aacagccgtg tttaattctc cgtttatgat aaaggcgcac aaaaacaaaa
      841 aatgcacctt atttacggat aaagtgcagg acaagaaagt aaaggaaagc atcgcaagcc
      901 aaacgtcgga atatgcactt gactggaaat ttcgctatga aaaaggcgtg gatgagttct
      961 ataatacata cacagaatgc ggtatctgta agctgggcat ccgagagaac tgctttgaat
     1021 ttgttccctg tctgtgcaat atggatgaga gaaactacag aaatgaaggc ggacagctgc
     1081 accgtaccaa aacgctggca cagggggatg actgctgcga ctttcatgtt acaaagataa
     1141 aatctgagag ttgatttttg gtttatcaga agtcatagca taaaaacagg aaatggggga
     1201 tggccttgta tccatggcta gagtctgtgt tagaggagca ataccgaccg gtttgcaggc
     1261 tgaggaaaac agagcggaat gaaatcgttc tgctggagca gaaagaacgc catgtgcgta
     1321 ttgtgaaacg catctttgcc ggcggttcag acgtataccg gaaactgctt acactgaaac
     1381 acaaaaaaat gccggaaatt ttagaggtta cagaatttgg tgaccgaaca cttgttctgg
     1441 aagagtatat tgacggtgtg acactttggg atttaatgca ggaagaggga tattacacgg
     1501 aaaaggcagt cagaagcctt atgtcagagc tttgcgatgt tctcgccatg ctccatgcac
     1561 agaatatcgt gcatcgggat atcaagccgc aaaatattat gatcgagaac aatacgcata
     1621 cgctgaaact gatcgatttc gattctgccc ggcttataaa accttgttat cccaatgata
     1681 cggaatatct tggtacagta ggatatgctg cgccggaaca gtatggcgag gttcagacag
     1741 acaccagagc cgatatctat tcgatgggaa tattgatgaa cgaactgctg acaaagaaaa
     1801 gaccctcgga actgctctat cccggaaagc tgcaaaaggt gattgtgaag tgcattcagg
     1861 tttcaccgga caagcgcttt caaacagcag agaaattgaa aaggagtctg cacggatgaa
     1921 aaagtgcaaa tattgtggaa aggaactcca cgaagatgcg aacttctgtc cctactgcat
     1981 gagaaagcag atcaatgcgg aatatgtagt agccaaaagc gtacatggac tgcgttttcg
     2041 atggcttatt ctggctgggg ctgttttgat cgctgctgtt tttgcctgcg tctggatcct
     2101 gccgaaaatt tatcagaaac cggatgcaca gcagtccgca aaaaaagagg attctgccat
     2161 tgactatgat gcctatgtgg ggacgtggtt cagcaaagaa atcagcagtg cggatgaatt
     2221 ttatgagagc ggcggttcag ctgtgcagat tttctcgatc gatgacagga ttgccgagat
     2281 ttctctgtca actgtacaga gcgctccggc aaatcgggct gcaacaacgg ataaaatata
     2341 cggggaagta gacggaaacg tcattcagtt ttcttttcaa aatgacggat ggatgagttc
     2401 cggtgagggg acgatcacgc tgctgaatga tacaatttat gcagaagtga tatataccta
     2461 taaagatccg tctgcgctgt ggtatctgga tcatgccaat attttttatt tgtgtgacaa
     2521 ggaaacgccg tcgctgtttg gataaatcgt tttgggaaac ttgggaatgc ctggtttgta
     2581 cagataaatg tctctctgtt tggaggaagt ggattgaaat accgttaacg ttgacggcag
     2641 catgaccgtt gctggtctct ctccatttgg agagagtgga ttgcaatgta aaagacaagt
     2701 tttatatttt acatagaaag ggataaacat gaaacaaaat ttgacaacag ggagtgttct
     2761 gaaaacagta ctggtatttg cactgccgta tttgctgtcc tattttttgc agacgctgta
     2821 cggcatggca gatctgttta tcacgggaca gttttacggc gtggacagta tcaccgccgt
     2881 ggcaaacggt agccaggtca tgcacatgat taccgtgatc ctggtggggc ttgccatggg
     2941 tgcaaccgtt atcattggac gtgctgtcgg cggaaaccgg atggatgagg caggaaaagc
     3001 cgttggaaat acggtgacgt tgtttatggt tttggcggct gcgttgatgg cggttctgtt
     3061 atgcctggcg agacctattg tcattctgat cggcacgccg gaagaggctg tggcagaaac
     3121 ggtgcagtat ctggtcatct gatttttggg aatcccgttt atcacagcct ataatattat
     3181 tagcgccatt ttccgtggca tgggagattc caaaagcccc atgtacttta tcgcaatcgc
     3241 ctgtgcggca aatatcgggc tggattatct gttcctgggc gcatggaaaa tgggcgctgc
     3301 cggtgcggcg ctggggacta cgctgtccca gactattagc gtggtgatct ctcttgccgt
     3361 gattctccgg aaaaatacgg gactgtctct gaaacactcc gattttcgcc cggatcgtat
     3421 gatgatgggg cagattctga agatcggctt tccggttgcg gtacaggatg gctgcattca
     3481 gatcgccttt ctgctgatta ccgtgatcgc caatcaccgt ggactggacg atgcggctgc
     3541 ggtgggaatc gtggaaaaga tcatcagcgc tttgtttatt gtgccgtcct ctatgctggc
     3601 gacagtctcg gcgctgtcgg cgcagaatat cggtgccgga aagcataagc gtgcctcgca
     3661 gacactgcgg tatgccacga ttctgacgac cgcctatgga atcgtgataa ccattctcat
     3721 ggaactggca gtgcagccgg tcatgcagct gtttacgccg gatgccgctg tagtggcgct
     3781 gggatgtcag tatatgcgtg gctatatcat tgacaccatt tttgctggca ttcactttag
     3841 tttcagcggt tattttgcag catacgggaa gtcctacatc ggatttctcc acaatatggc
     3901 ggcgatcgtg ctgctccgtg tgccggggtc ttatctggcg tccaggatgt tcccggagac
     3961 gctgtttccc atgggtctgg ctgcaccggc agggtcgctg ctgtctgtgg tgatctgcct
     4021 cggcgcattt ttctggatga aacgccgtgg aacgatagaa tgcgcatagc acataaaatt
     4081 ataccaaaaa acaacaccgt gtaaagcttg gaaattggct ttgcacggtg ctttttagca
     4141 tctgtcgggc ataattttgc tgctggcaag gcaattgtga ttttatacct cgctggaatg
     4201 ataaaacagg atacggtatt gccttggcgt gtatccggtc tcattgcgga aacattggat
     4261 aaaatggctc tgcgaggaaa aactgaaata gcttgcgatc tcagagtagg gcatatcaga
     4321 tgtagtgata agctgtgccg ccgcctgtat cttctgggac ataatgtagg aatgcagctg
     4381 tattccggtt tcccgataga aaagggtgga gagatatccc cggtggatgg agaccgcctt
     4441 tgcgatttct gcaatttgca gtttgtcatg gagatgggca gagatgtact caatggctct
     4501 cataacgtaa atggagtagg cgttttcctt tttctgtttc tgcattcgct tggtaaaatc
     4561 gactaccatc gtgttgtgca gtgcggcaac ctgggagact gtttgcagat gatccatctt
     4621 ttggatgaag atgtcgctga gggtataggc ggtttcccgt tccatgccgt gttccacgca
     4681 gatgcgtgtg atgagtgcga cagctaccac aa
//
LOCUS       DAWENO010000162         4711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6724_length_4711_cov_9.314003, whole genome shotgun sequence.
ACCESSION   DAWENO010000162 DAWENO010000000
VERSION     DAWENO010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6724_length_4711_cov_9.314003"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1130 a         1116 c         1335 g         1130 t
ORIGIN      
        1 tctggaacaa gcctatgcac cggagctgct ggaagagcag ttcctggtgt ttgcggcaac
       61 ggatgacagt gcggaaaatg cacgcatcgt ccgggatgcc caggcggcag gaaagcttgt
      121 ctcctctgca accgtcagtc cggacagcac accggatttt gccgtcccgg cacaccgccg
      181 ccacggggat gtgacgatct cggtggcaac tgagggcgat gcgccggcgc ttgccggtgc
      241 aatctgccgg gaactggaac cgaaactggc atcgtacagt gagatctgta cctgcctggg
      301 cattctgcgg cggacgtgga aagagaccat accggacgat tttcaccgga tgcagctgct
      361 ccggatgctg accgagccaa aggcgctgga actgttccag gaaaagggaa agggcgctta
      421 tctggcgtat gcctcttcgc tggcagatgg tgaaatgccg gctgtcaaga aagcgattct
      481 gatggtcagc tttgggacaa gctatgcaga cacccgggag cataccatcg gtgctgtaga
      541 gcgtgcagtg caggtggcgt ttccggagat ggatgttttc cgggcgttta ccagcggcat
      601 gattatccgg aaaatgcggc ggcagggaaa cgcagtcgat acggttcggg aggcgctgga
      661 aaagctgaaa cagcagggct atacccacgt atacctacag cccacccacg tgattcccgg
      721 cgaggaatac gaccgactct gtgcagatgc ggcggcgtat accggtgcat ttgcggtaat
      781 gcgcattgga aaaccgctgc tgacagagac agcagattat ccggcgctgg tacaggcaat
      841 ggagcaggac ggcgttatcc ggaaatctgc ggaaaaggca tatctgctga tgggacacgg
      901 cacgccgcat atcatcaacc aggcgtatcc ggcattcgat tactggctga aacgcagcgg
      961 ttttgacaat gtatttgtcg gaacggtgga aggctatccg acgctggaaa cgctgctgga
     1021 actgctgaaa gagcatcatt atcgagaggt tgtactggtg ccgatgatgc tggtggcagg
     1081 tgaccatgcg cagaacgaca tggctgggga tgattcagat tcctggaagt cggtgctgga
     1141 acggtacggc tatatcgtga cgccgcagtt gcagggactg ggcgcatacc cggctgtgca
     1201 ggcactgtat attgcacaca cagcgcatat gctggaacag tctccggaag gtggtgaaac
     1261 aagatgaatc ttcccaacga cccgatcatg ctgatgagct atctgaatac acagctccgg
     1321 gacaattacc cgtccctgga ggaactttgc aagtccatgt gcctggacga gacggagatc
     1381 agagcaaagc tggaaacagt aggctttgtc tatcagccgg aacgcaatca gtttgtataa
     1441 aattgcataa agttttcaga aaccaaaaaa gcggaatgcc gaccagttac agtcggcatt
     1501 ccgcttttct ttgcaaatat tcagcagtcc gagtgttcgc tgaggtaagc ttttaccgtt
     1561 ttcagcagct tttcatggca ctgctccaca gtacagcccg tgaaatccac catcagatct
     1621 gcgtattttt catacaaggg gcagcgctct tcatagaggt cttccagggt gttgcagccg
     1681 ctgtggcaga caacgccacg tgtgacaaga ttccccacac gctgtgccag aacgtccagc
     1741 tggtggtgca gatagacaac gatgccggtg gactttaagt actgcatggc gccgtcactg
     1801 tagacagcgc tgccgccggt ggagatcact gcacggttgg tgtggatgga ttgcaggatc
     1861 tttccctcca gtgtgatgaa accctctgtg ccgtgcttgt cgatcagttc catcagcggc
     1921 tgtccgtagg tctcgatgat gacatcgtct gtgtcgataa agtggtagga cagtccctct
     1981 gccagcgttt ttcccagcgt agtttttccg gatcccggca ttccgatcag tgtgatattg
     2041 tgcatttgtt tggcgctcct tttcttgtac ttgtagttcc cggcattttt gagaagtcca
     2101 ggttctgaaa atcaatccaa gagtctgtac cgcagcgttt ccggacaggc tcacagcagt
     2161 cattttgaag tgtccctact attttaacat atttcttaca aaaaagcaag tcatacagca
     2221 tcatctcaca aaaaaagtcg ttggaaaaac aacataatac agcagaagtt cggatagctg
     2281 aaaatgtgta caaaacaatt tacatctcaa caataaaaac agtggaaatc gttataatta
     2341 taatggtata ctaaaaacgt atgtaggagc gtcagtaagc cctgaaaagt ttgtgatagg
     2401 agctgaaaca aggtgaaaaa tcaacagtat caatatttgt gcctggtgga ggaactgcga
     2461 aagcggctgc aggacggcga gttgcagtcg ggcagtgcaa ttccgtcaga ggcagaactg
     2521 gcgaaagcgc tttcgctgag caaaagggaa gtgcgtccgc tgctgaaaaa tctggagatt
     2581 gcagggattt tgtccggcag ccgggatggt acatatacgc tggcgaaaga aatgtcggac
     2641 agtatgcgtg aactgatgca cgtgatgttt ctggtgcaga acatctcgcc gtttgaggtg
     2701 tgtcagatgc gccgttctat ggaactttct gcctttccga tggcatatgc acgccgggat
     2761 catctggatc tggacgaact gaaaaatctg ctggatgaat tccgctatgg aaacggtctg
     2821 gacagcatcc gggcagatga ggagatgcac cgctggctga taagagcatc cggaaaccgg
     2881 ctgatggagt gcgtgacgca ggggatctgg ggcatctgtt cggcgcagat caacttgatc
     2941 ttgtcggacg gtacggagga actgcgaaag gtgcaggcga gcatccatga gcggttctat
     3001 aagagctttc tcatcggtga tctgaggatg ggcttggatg ctgtccggga acactatgac
     3061 accattgaac aggcactggg tgagcagtcg ctgtacgacc tgggaacgca catcgcctga
     3121 caggctgaaa cggaaaggag aatgtgatga atctcttttt aacgctggca tttttatttt
     3181 gcattggcag cgtgctggga tggggcttgg aggtcatatt ccgccgcttt ttctcggcgc
     3241 accactgggt gaatccgggc tttctggtcg gtccctacct gccgctgtac ggctccagtc
     3301 tttgcgtgct gtatctgctg gcgatgctgg aaccgctcat gccgggcgac agtccctggc
     3361 tgaaaaagat actgcttttt gtggtaatgg cgctggcaat cacggcactg gaatatgtgg
     3421 cagggatgat ctttattcac ggcatgaaaa tcaagctgtg ggattattcc gaccgctggg
     3481 gaaatgtcaa gggcattatt tgtccgctgt ttacgttttt ctggctggtg ctgagtgctg
     3541 tatactattt cctcatccat ccgcatattt tgtcggcgct gcactggctg gcagaaaatc
     3601 tggcattttc gttctgcatc ggattcttct acggtatctt cttaattgac gtaggatatt
     3661 ctacccattt gatggtaaag attcgtgcgc ttgcagcgga gaccaatatc gtggtacgtg
     3721 tagaggaact gcgtgaggac attcaccagg cgctgcaaaa gaaaaatgtg cgccggttct
     3781 ttttccctat gcacggcggc gtgccggtgc gggaaatgct ggatcagtac gtaaagcagc
     3841 acttctcacg ggaaaaatcc cagggaaagt ggattcggct gccatccaaa aaagccaaag
     3901 agaaaaaatc caaataagct aaaacagccc gatactctca aagtgagaaa gcatcgggct
     3961 gtttttttac atttgctgga tttattcttc cggaggtgcc tgctttcggc tgcgtctgcg
     4021 gttttcggat tctaccggca gcacgtcctc gaacatggcg tagaacttca tggggataaa
     4081 acgatccatg tggcacttgc cgaagaacca tttccggtac tggcactgtt tgatgatatc
     4141 ctcgaaaaag gtgtggatct ccagacgctg ctggacatct acccccaggc agtccttcag
     4201 agatgccggc ggttcgtggg tgatgatgta gtccacctgg gcgttgtgct cattcagatt
     4261 gtggatggca cggcggatct cagcgtgtgt gggctgttcc cgctgccacc agttccgacc
     4321 ctgccggaat tcgaagtcct ggctgtgacc gccgccgaat gcgaaaaagg tgcggtcatc
     4381 aatggtgaag atctcgccgc gcatcatgtg aaaaatattc ggtgcgatca gatgcgccct
     4441 gccgccacgc catctgactg tgcgaaaact ctgttccagg atgtcgaaat tttcgtggca
     4501 gccgtctaca aaagcgatgg tgaacggcat ttcacgcagt tttttcagat tttgtttttc
     4561 ctggcgggaa ccgtcccaga taaaaccgaa gtcaccgcag acaatgagaa catcgccggc
     4621 tttcagcttt ttgaaaatgg gatttttgaa tcgtgacaga tctccgtgca tatctcctgt
     4681 aatgtatacc aaaaggaaaa cctccttgcg c
//
LOCUS       DAWENO010000163         4708 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6728_length_4708_cov_10.022781, whole genome shotgun sequence.
ACCESSION   DAWENO010000163 DAWENO010000000
VERSION     DAWENO010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6728_length_4708_cov_10.022781"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3911..3920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1267 a         1216 c         1250 g          965 t
ORIGIN      
        1 atccaggatg cggacgaagt gggtgtggca acggcaacag tcaagctgac ccggagctac
       61 tatgacacgc acgacaccac ctggaaactg gatgaggatt ttgtgcttgt ggcaacgggt
      121 acaccggtag caacggcaac gcctgttgca gccggaacgc cgactgcaag cgcatccaat
      181 ggtcactatc gctttgaaaa tctgccgacc tttatagaaa aggacggtca gagatatctg
      241 gttggctatc agatgcagct ttgcgaaatg ccggagggct acacagcaac gaagtaccat
      301 gcggcggcag acagcaataa ggacagcgat ctgatcgcag gcacgctgga tctccaggag
      361 ccggacaact tcttgatcct ggcgagcgca ttcagcggaa acacaccgta ttgctatcag
      421 gtacgtggcg tggattatga cattgtcaag gcggcaaata tcaccggcta tgacggcggt
      481 attgtacaga aggattccgg tgcgtccatc tccggcattg tctgggatga tgcagaccgg
      541 gatcatgaga tcggcgacgg cgagtccggt attgccaatg taaagatcat ccttcagcag
      601 tattattacc gcaatggaaa gtgggaactg ctgcccgatg cgaaacagac tgcctatacc
      661 aacgcagacg gagcgtatcg ttttgacgat ctgcctttgt ctgtgacggt agacggcgtc
      721 acatatctgg caggctatcg cctgtggatc gacagcatcc cggcagggta taacgctgag
      781 gaatatatca aggcgaataa tgaaaaatta tcgtccggtc gtttggaaat caagacagat
      841 aaccgcacac tgaatggctg cattgttctg ggaataccgg aagaaaagga tatcacgtcc
      901 ggaacttgtc tggatgggtt caacaccgcc aagggcgaag atgtggtatc tctgaacgtg
      961 tatctgaatc gtgatccgga agagactacg acgacaacaa caacagtcac cggtacggtt
     1021 acaacgacaa cggagacagc cgcaggctcg gcagagtcta cgacacagac cagtacagcg
     1081 gcatctgcag aaaccacttc tgcaacaggc ggtacagcac aaaccagtac aacaacggtg
     1141 cgtgtgacag ccggcacaac ggaaacgcag acggctgaaa ccaccaccac acataccaca
     1201 tcggcagcac atacgacagt aaccacgaca cacacagcag gcaataccac aacatcaaaa
     1261 cagaccacac atatcacagc acagataacc acaacaacgg cgaaagctac ggcatcgccg
     1321 gcaacacaca ccggaacaca gacgactacc gcaacgacac ataccacaag tcgcagaacg
     1381 acgaccagca cgaccagggc aaccggtacg gagacttctc agacaaaggg aacgacacgc
     1441 accacattga ccacaccgac tgctgttatc actacgacaa cgactaccac cacagcaaca
     1501 caccgcacca cttcttccgc agcatcgcag acaacggcaa cggttacgct ttccagcgca
     1561 ggcccgacca acacaacgac taccacacat accacaatcc agaccacaca aacgacctcg
     1621 aaacacacgg tgcagacaac gacatcccgt cagaacggca ccacatcttc tgcgactttg
     1681 acagaaacca gccgcacgac ttctacaaca gtcactgcga cggaaacgac cagttcaaag
     1741 ccggcaggca gcagtgtttc aagtgcaaca cacaccacac agacaacgac aaacaccacg
     1801 acaacagaaa ataccacttg tgagacatcg gatacttcta ccagcatccg acagactaca
     1861 tcccggaaga atcacgtgat cgtggatacc acaacgacta cgaccattac cacagacaga
     1921 gatcatgcag cagacaacag cacgcagacg acgacaacgg ctgccacaga tgtttctgca
     1981 caaacatctg tcaagaaacg aattccgctg ccgcagcggc agaacattcc gggaacgcca
     2041 aagacaggag aaagcacact gcatatcacc attttgattg cagcctgcgg attctcgctg
     2101 ctgctggcga tcctgacaaa gcggcgtaaa aagtaactgc gctttcggat tgatccggaa
     2161 cgcacagcaa aggagctgca tcgacttcgg gaaaccggag cggtgcggct ctttttgcat
     2221 cttaccgtcg gaaatcgacc acttgcgttt tagggcttgt tatccggtca gaaaaaaggt
     2281 ataatcaaga taacagattc cgaagaacag agaggacagg gatggatatg gaaaaatgta
     2341 ttgagattca aaatctgcaa aagagtttcg gcgcaatcca tgccgtaaac aacttatcgc
     2401 tgcaagtgga gacaggacag ctttttgcat tcttaggtgt caacggcgcc ggaaaaagca
     2461 ctacaatctc catgatatgc ggtcagctcc ggcaggacgc cggtcagatc tatgtgaacg
     2521 gcaggaatac gctgaaaaac atgaacgcca tcaaacagga tatcggcgtg gtgtttcaga
     2581 actccgtcct ggacagacag ctttctgtgc aggacaactt gaaaaaccgt gcggcgctgt
     2641 acggcatcac cgggaaggca tttcaaaagc gtctgtttga actggatgac ctactggagc
     2701 tgcggccgct tttgaagcag agtgtgggaa agctttccgg cggacagcgg cggcgcattg
     2761 acatcgcccg tgccatcttt caccgtcctc agctgctgat tctggacgaa ccaaccacgg
     2821 gactggaccc gcagacacgg cagacggtct ggaatgtcat ttccgtcctg caaaagaaaa
     2881 agcacatgac cgtttttctg acgacgcact atatggaaga ggctgccggt gcagaccgaa
     2941 ttgtgattct ggatgaggga aagatcgcag cggagggaac gccgctggag ctgaaaaacc
     3001 gctataccgg ggattatatc acgctgtacc atgcaaagga atccgcagtg gcgtctttgc
     3061 agatgcccta tgagacatac ggcgaggcgt tccgcatatc tgtgccggac acccggacgg
     3121 caacaaaact catcgtacag catccggaat tgttccagga ttatgagatc atgaaaggca
     3181 gaatggatga tgtattcctg gctgtgacag gaaagacgac aggaggtgct gcttatgctg
     3241 aaagccatgg tattgcgtaa catcaagctg tatttcaagg acaagggaat gtttttcact
     3301 tcgcttatca cgccggcaat tctgctggtg ctgtatgcga cttttctggg gagtgtttac
     3361 cgggattcgt ttaccgcaaa tcttccggac ggcttttcgc tttctcaaaa gctgatcgac
     3421 ggcgctgtcg gggcgcagct catttcttcc atttttgcgg tcagctgcgt tactgtgccg
     3481 ttctgttcca atatgctgat ggtgcaggac aagatcaccg gtgcccggaa agatctgacc
     3541 atttcgccgg taagcagtgc tgtcttgtcc ggcagctatt tcctgggtgc ggcgatttct
     3601 tcgctgctgg tatgctttgc ggcgctggcg ctgtgtctgg gatatgtgcg gacgatcggc
     3661 tggttctttt ccggaaagga tatcctgcta ctcagtgcgg acattgtgct gctggtgctt
     3721 ttcggcacgg cgctttcgtc gctgatccac tttttcctca gcacccaggg gcagatctcc
     3781 gcagtgggaa cactggtcag cgccggctac ggctttctct gcggtgcata tatgcccatt
     3841 tccagttatg gtagcggact gcaaaaggtg ctgtcctttc tgcccagcac gtatatgaca
     3901 gcgctggtgc nnnnnnnnnn gctggtgcgg aatcacacca tgcgtggcgt tctggcgcag
     3961 atggagacag acggtgttcc gaaagcgctt gtggatgcca tccgggagtc gctggactgt
     4021 accgtaaaat ttgccgggaa aacggtgacc gtccctgcca tgtatgcgta tatcggcggc
     4081 gcagttctgg tgctgctggg cgcatatctt gccgtgcatt atctggcaga aaggaaacga
     4141 gtccattcat gaaagtgacc attgaaaacc tgccgccagg agaagaggcg gagatcatca
     4201 tacgtgccaa tacactgtcc cagcgtgtga tggatctgct ccatgcactg caaatggaga
     4261 acgaaaaact gatgctgtat cacgaggatg gcagcatgag cgtgctgcat ccggacagtg
     4321 tgtattattt tgagtccgtg gataacaagg tatttgcata tggaaaatcg gaggtgtcgg
     4381 aattgaaaca gaagctttat gaactggaac agcggtttgc cgggacggat tttatccgaa
     4441 tctcgaagtc gatgattttg aacctggcaa aagtggaacg gtttgcgccg tcattcagcg
     4501 gacgtttcga ggcaatcctg caaaacggcg aaaaagttat gatttcacgg cagtatgttc
     4561 ctgctctgaa aaaacagctt ggactgtgag gtgcggcgat gaagaaccga ttcaagcgtt
     4621 ttctttccgt gacgacagcg gctgtgactg gtacgctgat cgcctgcatc ttttggtatg
     4681 gaatctactg tctgacgggg cagcggca
//
LOCUS       DAWENO010000164         4707 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6729_length_4707_cov_8.243981, whole genome shotgun sequence.
ACCESSION   DAWENO010000164 DAWENO010000000
VERSION     DAWENO010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6729_length_4707_cov_8.243981"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    936..945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3753..3762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1071 a         1364 c         1282 g          970 t
ORIGIN      
        1 ctatttgtat catcccactc gaaaaaggag cgatttccta tgattacctg tagccattgc
       61 ggtcagatcg cagcagacgg ttcggcgttt tgccctaact gcggcgcagc tcttcctgca
      121 ccgtattttc agcagcccca gcagcccgtt caacagcctg tttatccgca gccgcaaatg
      181 ccgccttatc cgggctatcc cacaccctgc gaacagcccg gcaccaatgg aaaagccgtt
      241 gcatccctgg tcatgggaat ctgctccctg ttggtgtggc tgctgtttct gacaaatgat
      301 ttcgatgcgt tcagctttct cagcatcccc ctggggatca tcagcattgt catgggcgct
      361 gtagcaaaga aagagatccg gcgtacaacg cccggcggca gcactgccct cgccacagcc
      421 ggcatcgtct gttccattat ctcgcttgcg ctggatgtgg tgttttttgt actgggtctg
      481 atctttgttg tgatccttgg catggctctg gcaagatttt aagcatcctg tacacatccc
      541 gatacgaaac aggtcctgtg acctgtttcc ataaaccaaa ccaattcatg gaggtatttt
      601 cttatgttga cttgtgagac ctgcggcaaa gttctgccgg aaggcacttc gttctgcccg
      661 aactgcggta tgcctgtgga acatacagac agcgctgttc agcccgacgc acccaacgct
      721 ccggcaccgg atacagctgt accggacacc gacagccagt tccagcagcc ccaggctgcg
      781 ccggaatcca atccctatgc tatgccctcc ggcggttctg ccccggcatc cggcagcatt
      841 ccggaacaga gttatcccgg aacttatccg cccggcggca attccggcta tggcgcacag
      901 ccgaaccagc cccagagcca gccgcagccc catccnnnnn nnnnncggga aacggcggct
      961 atattccgcc gcagcagcag ggctatccgg gcggttaccc gaattatccc cagaactatc
     1021 cgaacaacag ctccaacggc aaggcaattg ccggactggt gctgggcatt ctctccatcg
     1081 tgatgggatg cgtctacggt tccggtctcc tcttcggtat cctgggtacc attttctcct
     1141 gcatggcacg gaaagaggac agcagtaacc cgctgcaaaa caagaaaaac agcactgcca
     1201 ccagcggtct catctgctcg atcataggta ttgttctttc tgtgatcgtc cttctcatcg
     1261 taatcattgc cgtggcagtt tccgtggaaa caggagacta caccaccccc ggctactatg
     1321 gctccggtga ttaccaggat gcctgccagc tcttcttcgg actgctccgc taatgtacta
     1381 tgaccttcct gcgcacggac ttcggtctgt gcgctttttt gtcagataat gccgggaaac
     1441 agcacgtgtt ttcaaattat tccttgacag acattctgca atatgataga ctaaaagcag
     1501 taccagagcg tattcatatt cctatgggaa cacgctctga aatacagacc gaaaggaaac
     1561 gataccacca tgtttcgaac ctatgacctc agcggcatct gggacttttc catggcacaa
     1621 atggcaaaag gcacatttcc caccgctttt gatgatacga tcgcactccc cggcacaaca
     1681 tctctcgcca aaaaaggcgc acccaatccc aaccgggagg caggctttct cacggatgcc
     1741 tacgcctttg agggacaggc gtggtttcgc aaagagatct gcattgaccc ggaactgttc
     1801 gacaccgata tgggaatgct ccccatcaag ctgaccctgg aacgcacccg gatgaccacc
     1861 ctctggatca acggcaggct tgtgggcagc tgcaacagtt tatgcacgcc ccacgtctat
     1921 gacatcaccg attttgtgac caagccgctg gtggagattc tggtgctggt ggagaacacc
     1981 ggctatccca ccaaaggcgg acacctcacc tcgccggaca cccagtccaa ctggaacggc
     2041 atcaccgggc agatgaccct ggaagtgttc ccggaggtct atgtagatca tgtccaggca
     2101 taccccgacc cggaacacaa aaacgtcacc cttaccatgc agctccacgg ggcatccacc
     2161 gcaagctttt ccgccactgg cagcgttgtg gaggacaacg aagaggacac cattccgctg
     2221 ttccaggcgc agctgacggc agacgaaaac ggtatggcat ccgtgaccat tcccctggac
     2281 gaggcgtatt atgaatggag cgaataccac ccggcgctgt actatctgtt ctacaccatc
     2341 gacgaggcag actgcggcag cgtcagcttt ggaatgcggc ggttctgcgc cgagggcagc
     2401 cgtttcacca tcaacggaca gcccaccatg ctccgtggca agcacgacgg actgatcttt
     2461 cccagaaccg gctatgcacc cacggatgta aacgagtggc tgcgtgtgct gagcattgcc
     2521 aaaagctacg gcatcaacca ctaccgtttc cacacctgct gtccgccgga tgcagcgttc
     2581 actgcggcag atgtgctggg catctacatg gaaccggaac tgccgttctg gggaacgatc
     2641 gccgctcccg gcgaagaggg ctacaacgag gcggagcaga actacctcat cgaactggga
     2701 gacaagatgc tggacacatt cggcaaccat ccgtcctttg tcatgttctc cctgggcaac
     2761 gagctttggg gcagcccgga acggctggga gagatcctgc ggcactacaa ggaaagggac
     2821 agccgccatc tgtacaccca gggctgcaac aatttccagc attttccgct gatggtgccg
     2881 gaggacgatt actatgtggg cgtgcggctc agcaaggaac ggctgctccg tggttcgttc
     2941 gggatgtgcg atgcaccgct gggtcacgtc cagacagagc gccccagcac catgcaccag
     3001 tatgacgacg tgatctttcc caagcagacg gagggcgagg gcgcatccga cacggaagag
     3061 atcgagatcc agtacggcac cggcgtgaaa aaggtgcagg tgagcaagac tgccggcgga
     3121 ctcattccca ccaagccggt ggtcacccac gagatcggac agtacgaggt ctatcccgac
     3181 ttccgggaga tcgacaagta caccggaccg ctgaaagccc ggaacttcga gattttccgg
     3241 ttccggctcg gtgcaaaggg aatgctgcgc caggcggaca agttcttccg gtgcagcggc
     3301 gcactggcgg ctgcctgcta taaggaagag atcgaggcgg caatgcgctc gcagtatatc
     3361 gccggattcc agctgctgga tctccaggat ttctccggtc agggaacggc gctggtggga
     3421 atgctggacg cattcatgga cagcaagggg ctgatctcgc cggaggaatg gcgaatgttc
     3481 tgctcggact gtgtgctgct ggcacagttc ccgtcctatg tgctgattac cggcgaaatg
     3541 ttcgtggcaa aagtctccat gcgaacggtt ctgccccagt ccggcacggc agaatggatg
     3601 ctggttcggg aaaacggcga caccctgggc aaggggacat tcccggtaca ggcggaatcc
     3661 ggtctgacgg agatcggcag catcttctgc cggatgccgg aggaattgca gcgccccgaa
     3721 cgggtgcatc tcatcctgtc gctgacggac acnnnnnnnn nntatgacct gatgctctac
     3781 ccagccattg ccatgccgga gctggagagc cgggcggagc tgtgcatcac ggaaaatctg
     3841 gacacggcgc tggcggcact ggcagacgga aagaaaacgg tctttctgcc gagagaaact
     3901 gccgagagca ttccgggatt ctactgcacg gacttctggt gctatcccat gttccgggac
     3961 atctgcaact ggatgaaaaa gccggtggca gtgggaacca tggggctttg cattcaggac
     4021 gaccatccgg cgctggcaga gttccccacc caggaataca gcacgcccca gtggtacgac
     4081 attgtgacgg cggcggactg caccatcctg gacgacacgc ccagcgagtt cacgcccatt
     4141 gtgcagatga tcgacaactt tgagcgcaac cataagctgg gaatcgtctg ggaggcgaaa
     4201 gtgggcagcg gcagcctgtt ggtctgcacc agccgcctgt ctgagatcgc cactcgtccg
     4261 gaagtgcgct ggctggcgaa gagcctgctg gactatgcgg catccgaccg ctttgccccg
     4321 gcgcagagtg tgacggcgca gcaactgcaa aagtggttcc ggaaaggcga atgacaccga
     4381 ctgccctgcg gaaaaaatga aaaaatcccc ttgacaaacg gggcgtgatg acgtataata
     4441 gagatagaaa gagagcatac cagcagacgg tttgctcccg ctgcatcaag ttaaaaagat
     4501 ttaaccgtca cagtttggtt gcttggggcg gttatttctt tttatggtga tgctttttac
     4561 tattcttttc attgtagtaa atgtacaaga tcaaagttgc caaatccaac agaaaactgc
     4621 cgatctttag caggtcaccc agggtgatat actgatccat cataccgatc acctcccatc
     4681 tgttcaaatg agcagtggga gcagaaa
//
LOCUS       DAWENO010000165         4697 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6742_length_4697_cov_8.567859, whole genome shotgun sequence.
ACCESSION   DAWENO010000165 DAWENO010000000
VERSION     DAWENO010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_4697_cov_8.567859"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    599..608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2177..2186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4428..4437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1144 a         1071 c         1368 g         1084 t
ORIGIN      
        1 aggacagctc ctactactat gaaggggcaa agggcatcaa aacaggtaat ctgagcaaat
       61 ccggaagaag catcattacg gaggcaacgc aggatgaaaa tacatatctt gtaattgcaa
      121 tgaatgcgcc gtttgaggat tcggacggca agctgcaata ttatcacatt gaggatgcca
      181 caacactgct gaactgggcg ttccagtcgt ttgcctatac cacactgctg gaggatgacg
      241 aggaaacggc agaggtggaa gtgatgaact cggacggcaa ttcctacgtg ctggtgcatc
      301 cgaaggagga ctgcatcctg ctctggtgca aggatgtgga cgtttctgcg gtgcagaagg
      361 tgatacacct ggatcaaaac gtcatggcac ctgtgaaaaa aggcgacaag ctggggacaa
      421 tggaactgaa gttctccggg gaagtgatcg cagaagtgcc gctggtggcg gtttctgatg
      481 tagatcggtc gttcaccaag ttcaacgcct atgcgctgga gaatttcccg aactcgccgt
      541 ggtttacggt gggaatcgtc gccggtgctg ttctgtcagc actgtacatt gcgctgtgnn
      601 nnnnnnnnac gcaacggaat tccacgaccg tccgcagcgg aactggaaac gctctgacac
      661 agtattttat catggtccgg agtccagaac ggaacacgat ccggatgcac agaaaaatgc
      721 agaagaaaag gaaaaagagc tgacaggtgc aaaccggaaa taaaggggat gcagttatga
      781 agatcgccat tgcgcagaca gagatctgct ttgaagaacc ggaaaagaat ttgcgtactg
      841 ctgaaagctg gatcgaacag gcagcacagg aaggtgcaga tgcaatattt tttccggaga
      901 tgagctttac cggattttcc atgcacgtgg aaaaagtggc agcgtatgtg aacccgattc
      961 ggcagacaat gtgccggctg gcagcgcagg aacatatagc tataggatac ggctggactt
     1021 cctgcaagtc cggcggcaaa ggggaaaatc actacacaat tctcggcaaa tccggggaag
     1081 tactgtcgga ttatgtgaag atacatccct tttcctacgg cggggaagag cggttttacg
     1141 acagcggcac ggaaattgtt cacacggtac tggaagactt tccggtcagc acgctgatct
     1201 gctatgattt gcgttttccg gaactgttcc gggcggcagc acagaccagc cggctgatcg
     1261 ttgtcccggc gaactggctg caccagcgca cggaacactg gctgctgctg ctccgggcga
     1321 gagctatcga aaaccaggta tatgtgctgg gtgtcaactg cgtcggaaag cagcagtcct
     1381 acacctttga cgggcagagc tgtctggcag acccggaggg aaatttgtgc tgtgtctgcg
     1441 gtgatgcacc ggaactggca tttgtgactg tggagaacga cacggaacaa ttccggcgtg
     1501 cgtttcccac ctgccgggat gtgaaactgc cgttttatac ggaattgtat cagaatcttg
     1561 tgagggaaga ggaacatcaa tgattgaggt ttttcagccg ccccggatca cacgggaaga
     1621 gactgcctat aaaatgctgt ttcatgtttt tgcggctttt gcagcattgg cggtttccat
     1681 ggctgggttg ttttattcca tggctggaga ttcccgaaag aaccgggatg cgctgcataa
     1741 agtcacgtct ccggagacgg aattgtgggc gattattgtt ttcgctgtgt ttgtcttggc
     1801 agctgtctat ttatcggttg caggggttgt gctgtcccgg cgagtgagag gacagtttgt
     1861 gcggtgggtg aaaaaaggcg gcaaactgta ttttgtgtct gcgagtgcct caaatgtcag
     1921 agcaagcggt tcttccagac ggatttccag ttcgctgaaa ttgcaggacg catcgctgaa
     1981 cgttttgaat gattccgggg ctttggagga tatattaaac ggaaaaactg catatgcctt
     2041 tatccaggca ataccggtga caagtgtcac ttgggttcgg cagaggaaac gtggcacaaa
     2101 ggtgtatttc caaaacaaaa aggtatttct atctaagaaa atgtacgggt atcacgtact
     2161 tctgcatctg ctggaannnn nnnnnntgaa aacgctgaaa tgcgttcatg gctgcctttt
     2221 gctgtattct gtaagtcaaa gttttagtgt tccggataag tgacttccat tgccttctgg
     2281 aaaaaggcgt gcatctccgg gaatttgtcc cggatgcgca tgggcttgat ctttgtgttg
     2341 gtaaagcagt gggaagaggt ggcaactgca caaagctgcc cggtttccac gcagaggata
     2401 tcataccgca gttccagctt gaaaccgttg tatttggtga tgtaggagta aactgccaga
     2461 tgctcaccga agtgcaggga gtgcttgtat tcacaggtga cgcccagcac cggcagcata
     2521 atgccttctt cttccatctg gttatagccg acgccgatct tgtccatgaa atcaactctt
     2581 gcctcttctg ccatgtggat gtagttggag tggttcacga tgtccatgcg gtctgtctca
     2641 tggtaaaata ccatgcgggt atacggtgtg atttccataa tacggactcc ttttctcgga
     2701 tgtgtttccg gaaactgcct ttttgtttcc gtggacacag aaagactttc tttttagtat
     2761 agcacaactt cttcggtttt actggttctt tttgtgtcca gtgtgttaaa gaaacgctgg
     2821 ataaggcata tctgaggcac aagctttacg cagtgttctt cgaaatgcag agaacaggac
     2881 ggaaaaacga tttttttctt cccggtattg caatttgtca ctttttcggg tataatataa
     2941 agtaagacac tatgtcagca ggaggaaaga gcatgaagaa gtggctgatt caccagccgc
     3001 cggaagaggc ggcaaaacag atccggagcg gcagcgatct gtccatgctg tgtgcacagg
     3061 ttctggcatc ccgtggaatg cacaatgtgc aggaagcggc ggcattttta cagtgtgacg
     3121 gtctggcaga tccgtttctc accgcagata tggaaacggc ggcagattac atcaaccagg
     3181 caatgtacag cggcagccgc atctgcattt acggagacta cgactgtgat ggtgtaacag
     3241 ccacggtgat cctgtactct tatctactgg aactgggcgc agatgtaacc tatcgcattc
     3301 cggaacggga tgagggatac gggctgaata ccaatgcagt ccgggaaatg cacgaggacg
     3361 gcgtggaact gatcgttacg gtggacaacg gcatcactgc gctggaagag gcggaactga
     3421 ttgcagagct gggcatgacg ctggtcatca cagaccacca ccagccgctg gaaacgctgc
     3481 cgagagcggc agcgatcgtg gacgcacacc gggcagatga tacttcgccg tttcacgacc
     3541 tctgcggtgc gggcgtggcg ctgaaactgg tggcggcgct ggacggcggc gatacggcaa
     3601 tggcgctgga gcaattcggg gaactggcgg caatcgcaac tgtggcagat gtggtaacgc
     3661 tttccggcga aaaccgctat ctggtgcagc tgggactgcg gcttttggcg aacacagagc
     3721 gtcccggact gctggcactc ctggacaaaa gcggactgac gggaaaggcg tttacttcta
     3781 cttccattgc attcggcatt gcaccgagaa tcaacgccgc cggacggttc ggctctccga
     3841 aaacggcggt ggaactgctg ttatgtgaag atccggatga ggcggcagaa ctggcggaag
     3901 agctggaacg gcgcaaccag gcgagaaaag cggaggaatc cagaatcctg gaagagattg
     3961 ctttgcaggc ggcagaacag ccgcagctgc tgaaagaacg tgtactggta tttgccggag
     4021 aaaactggca tcacggcgtt atcggcattg cggcatcccg tctggaagag cagttcggca
     4081 agccgaccat tctgattacc atagaaggcg atcaggcgag agggtcaatg cggtcgttcg
     4141 gcgaattctc tgcatttcgc tgtctccatg cctgcagtga tctgctgtcc cggtacggcg
     4201 gtcaccccgg cgcaggcggt ttttctctgc cggcagacca cgtggaactg ttccgaaagg
     4261 cagtgcagca ctatgcaaaa gaggcacatc ccaccatgcc ggtgctgacc ttagaggcag
     4321 accggctgct gatgccccag gagatcacca tggagaatat taccagcctg tcggtgcttg
     4381 cgcctttcgg tgagggaaat cctacgccgg tgtttgcgct ttgtcatnnn nnnnnnnccg
     4441 ctgtccggcg gaacgcattc caagcttaaa attacttacg gcggcatggc aatggatctg
     4501 ctgctgttcc ggacgaaacc ggaggatgtg acgctgaaaa acggcgatat ctgcgatttt
     4561 ctggtcaaca tagaggtggg cagttttcag ggcagaccgc agctttctgt gatcgtcaag
     4621 gactatcgga aaagcggtct gaaacaggcg aaatattttg ccgcacagag tacatatgaa
     4681 caattttgcc gcggcga
//
LOCUS       DAWENO010000166         4682 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6765_length_4682_cov_10.760104, whole genome shotgun sequence.
ACCESSION   DAWENO010000166 DAWENO010000000
VERSION     DAWENO010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_4682_cov_10.760104"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1101 a         1317 c         1283 g          981 t
ORIGIN      
        1 cagatggatg cgttcctgga cagagccgtc atgtcccata tcgccaccgt caccatcatc
       61 cacggcaaag gcacggggct gctgcgcaag gcgatccacc agcggctgaa acagctgaaa
      121 tacgtgaaga acttccgtct cggcacattc ggcgagggcg aggacggcgt gaccatcgtg
      181 tccctggagt aagccatgga gacgaaacca tcacagcgtg aacggctgct gcgctatgcg
      241 gcggaaaact acggcacaac gccggaatat ccctgggacg gcacgccgga tgcggcagtg
      301 ctgcgccacc cggggaatca gaaatggtac ggtctcctca tgcgcatccc gtcggaaaag
      361 ctggggcttg cgccggacgg aacggcagac atcctcaatg tgaagtgcag tcccctggag
      421 accggctcgc tccggcaaaa gcccggcatt tttccggcat accacatgaa caaaacccac
      481 tggatcacca tactgctgga cggcacactg ccggaaccgg aactgttctg gctgctggac
      541 gaaagctata cgctgaccat gccaaaacca cggaaacgaa aagaggtaca tccatgaaga
      601 tctggaacgc aaccatacat accatgaccg atgccggcat tctggaaaac ggctatgtct
      661 gcatcgagaa cggcaagatc accgaagtcg gcacaggcac accgtcagtg cttgcgccgg
      721 aggatattga cgcagggggt tcggttctgc tgccgggctt tattgatgcg cacacccacc
      781 tgggcattat cgaaaacggc atcgcctttg agggcgacga ctgcaacgag gcgaccgacc
      841 cgtttacgcc acatctgcgt gtgatcgacg gggtgaatcc gctggatcac tgctttgcag
      901 aggcaagggc agccggtgtg acaacggtca tgacctcacc cggcagcgcc aacgcctgcg
      961 gcggcaccat ggcaattctg aaaaccgccg gacggatggc agaccggatg cagatcggca
     1021 cgggcgtgaa gtttgccctg ggcgaaaatc ccaagagcgt ctacaacgac cgggacgaaa
     1081 cgcccatcac ccgtatggcg acagctgcca tgatccggga agggctggcg aaagcgcagc
     1141 gctatcagca ggaactggac tttgcagaga gcgacccgga cgagcatatg ccggactatg
     1201 acgcaaagtc tgaggcactg ctgcccctgc tggaacggga tgtgaaagcc catttccact
     1261 gtcaccgggc agacgatatc tgcaccgcca tccgcattgc cagagagttc aacctggact
     1321 atgtcattat ccacggcaca gagggacatc tgattgccga catcctggcg gaggaaaatg
     1381 ccaaggtcat tgccggtccg atcctgtgcg accgctgcaa gccggagatg cagaacctca
     1441 ccatctccgg gccgtctgtg atgcagaaag ccggggtcaa gctggcgctc tgcaccgacc
     1501 acccggtcat ccccatccag tacctgccca ccacagcggc tctggcagtg aaaggcggca
     1561 tgaaacggga agatgccctc tatgccatca ctgccggcgc agcggagatc ctgggcgtgg
     1621 aaaaccgtgt gggcagcatt gcgccgggca tggacgcaga tatacagctg taccacggcg
     1681 acccgttgga cctgctgtgc gacccgtgga tggtgctgat cgacgggaag aatgtattgt
     1741 aatttgtaat gtccatatca tcatcaggcg aaccctgttc cccacgataa aacgaatcaa
     1801 catcaaaaaa agccgtcctt atcgggcggc tttttctgtg ggtgaaaaaa atttttccga
     1861 aagtgagata ttttgcccct gccgatcgtt ataggggtga agcgcttcaa aaagaacctg
     1921 tgaaaaagga ggtgttccgc atggacgagg cagcattccg catcaagcta aagcaataca
     1981 aggataccgt ttaccgcatc gcctatacct atttgcaaaa caccgcagac gcagaggaca
     2041 tttcgcagga gacctttctc aagttctacc tccgggagac accctttccg gacgagggca
     2101 gcgaaaaggc gtggctgatc cgggtgacca tcaacgcctg ccacgatctg cggcggtcgg
     2161 tgtggagcag aaaccggacg gaactcccgg agaatctggc agattccttt gaaacgccgc
     2221 aggacaatgc actgtatgcg gcagtgttct ctctgccgga aaaataccgg gtggtcgtcc
     2281 tgctctacta ctacgaggac tattccatcc aggaaatttc ggagatgatt cagcgcaatc
     2341 cctccaccat acagacacag ttggaacgag caagaaaaaa gctgaaaatg cagctggaac
     2401 agaaaggtgg gtttcactat ggacaaacgg caatacaagc gtgtcatgga acaaattcag
     2461 atgtctgacg cctgcgagca gcgtatcctg gacgcagtgc accggcagga aatgccgaaa
     2521 cggaaaaaga caattaccct gcggaaaaaa gccggcatcg ccgttgcagc agcagcctgt
     2581 gtggcagcgg tcagcgtctt ttctgtggca gccgcacaga acaccagtct gctggagatg
     2641 ctgttcccgt gggcagagtc ggtagatacc ccggaaaccg cagtgttcac caacgggcag
     2701 atcgaatcct tcaccgtaga cggcatggac ggtctggaca ttacgcccat cggcgtggtc
     2761 aacgatgcga aatacgttta tctgatgatg cgtattgttc ccagtgatcc tgatttcaac
     2821 cctgcagaaa gatatatcag cacttacatg gactttactt ccaatctcgt atatccgcag
     2881 attgagggaa tcgacaacat ttatgcgtta aaagatgatg atccctatcg agtggaggaa
     2941 agcggctttt ccgtacaaac agaagtaccg gaatcagacg gctcgtatac tgcattctat
     3001 cagctgaaat ttgcaaacgg cattactttc gaccatctca attgtatcat tggactgacc
     3061 gatatgacag aactatcaga agcgcaaaac ggatatgcag aagccgatca gaccgaactt
     3121 atgggaacca tttccacttc ttttacgctg gacgattctt tgcaggatca gcaatgcgac
     3181 tttgaagaac ccatcgccgt tccaaatgac aacgggaccg gctatctgaa gcatctggat
     3241 atccaggcat tttctctgca cctttccggc accggagaga tggcgctgtt cggttcctac
     3301 aatgaacctt atgcggcaac catcacatat gcagacggca gtacgttcac accggattgc
     3361 aaaaacagcg gcttgacaca aacctatctg gagaataccc tttgggaata caacattgat
     3421 ttcgataagc ccattaatcc cgaaggcatc gtctccatcg aatttggcga cacagtcatt
     3481 cctatccact gacaactcca tatactcttc ccgacaccct gctttttctc cggcagggtg
     3541 tttttctgtc tgggcaaaaa aatccccgga caaacatcca ggcgatgtct gtccggggat
     3601 tggtttattt tatccttggt gagcgatatc agccctttct ccgcttgcgg ttatagccgc
     3661 cgctgcgtgc gtgctggtta tagccgtgac gggactggcg gtgctgtccg ccgtttctgc
     3721 cgccacgtcc gctgtgagag ccgccacggt ggtcgttatc acgctcttca tagcgccgca
     3781 cggaaactgc cttgccgttg attttctcgt cctgcatggc atcaatgaca aagtcggtct
     3841 ctgtctccgg cacttccacg gtggtgtact ggtcgtgaat gtcgatcttg ccgaattcgt
     3901 tgccagccat gcccgtcgcc tctaccaacg cacccaggat aaagttgggc gccatccggt
     3961 ccttgcgtcc ggcgttgatg tgtacccgaa cggttttcgc tccggcacgc ttgcccttgc
     4021 ggatcggctt tggcacatcg aatgtgggca ggttcttggt ggctgcgtcc agctgtttct
     4081 gcaccagcgc cgccagcagc tgttccggag acgatgcctg tgccagcagt tcctgcgcca
     4141 gcgccagtgc ctcttccgat gacggctgtg ccgccagttc cagcagctgg gtgcgtgtct
     4201 cttccgttgc cgatgcccgg agttccttgg aggtcggcac aggaatctcg gtgatgtccg
     4261 ccttggtata ccgggagatg tcacgcatct ccatttcctg ctttctgccg ctgaggatat
     4321 tgtacgccgt accggtcttt ccggcacgtc cggttctgcc gatgcggtgg atatagtact
     4381 catgatcctg gggcaggtcg tagttaaaca cggcatccac gcccttgaca tcaatgccac
     4441 gggcagccac atcggtggcg atgagaatgg tatttctgcc gctcttgaac ccgttcatga
     4501 cctgggtgcg ctggagctgt ttcatatcgc cgtggagacc tgcggcagaa aagccacgga
     4561 gacacagcgc ctcagtcagc tcgtctacca tcttcttggt gttgcagaag atcatggcag
     4621 acttgggctg tttcgacagc aggagcagct ggagcgcatc ggtcttgcgt cccatgggaa
     4681 cg
//
LOCUS       DAWENO010000167         4643 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6825_length_4643_cov_7.900828, whole genome shotgun sequence.
ACCESSION   DAWENO010000167 DAWENO010000000
VERSION     DAWENO010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6825_length_4643_cov_7.900828"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2819..2828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3625..3634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1016 a         1260 c         1155 g         1192 t
ORIGIN      
        1 tccatccgca ccggtgagca tctggggttc ttgccggcag agaacgacag taccaacgca
       61 gcagccattg atgctctgcg aaatcacttt tccccggagt ttctgggacg gctggatgag
      121 gtgctgtggt tccagaaact ggagagcgac agcctggcgc agattgcccg gagaatgctc
      181 cgggaattgc agcagcggct ggcgcacatg gagatcgcca tggaattcac ggaggatgca
      241 gtccagctgc tggcgaatgc gcccaaaacc aagcagtacg gcgcacgtcc catgaaacaa
      301 tatctggcgc agatggtaga aaacaccatc gcagacaaga tgctgcgcag tgaggtgcag
      361 tccggtgatg agatctgtct gactgcaaaa gaacgacggt ttgtggtgct ggacaaggtg
      421 ctgcctaccc ggtaaccgat tttatacccc aacaaaaaac caggcaatgc gttttcacat
      481 tgcctggttt ttttagtttt ccagtgcgaa caactcgttc agatagtgga tgccggtctc
      541 tgtccggaaa atcttatccc ggaacgattt gatgtttctc ttcggcagac cctcttcgta
      601 ggcgtttacc ataaagtccg cctcaatcag aatttgcaga tccacgtcat caatcttatc
      661 ataagaatga tggtgcgcca ccatatcgca gacacggttg atgagcgccg gcgaaaactc
      721 cagcgccatc agcatggctc ttgcgatcgg tggcccttcg atctcctggt actctccggc
      781 ggcagaacga tatttctttt ctgcggcatg gatgccgata tcatgcacca gagcagctgc
      841 ctcaacgatc tcacgctgct ttgcacccaa ttgttcttct tcggcgatca ttttcgcaaa
      901 ggcatatact ttcatgaaat gctgtatccg tttgggatcc ccgctgtaca atgtgaccat
      961 tgccattgtc aactgactca tcgatgccat atgactccgc tcctttcacc attacggttc
     1021 acctgctttc gctatacagg ctttttattg tcaaattttc ttaaagctgg tctgcaatat
     1081 cgtagatcag acgctcggac ttctcccagc ccaggcatgg gtctgtgatg gagcgtccgt
     1141 atacgccctc gccgatcttc tgtgcaccgt cctcaatata gctttcgatc ataaagcctt
     1201 tgaccagcga tgcgatttcc ttgctgtggc ggcagctgtg caggacttcg ttgcagatac
     1261 ggatctgttc catatacttt ttgccggagt ttgcgtggtt tgcgtccacg atcacagccg
     1321 gattctgata gttcttcttc tgatacagat catacagtgt aatcagatct tcatagtgat
     1381 agttcgggag tgtgcgtccc agcggatcta ctgcgccacg caggatagcg tgtgccagcg
     1441 gatttccggc agacttaact tcccagccac gatacaggaa cacatgggat gcctgtgccg
     1501 cagtcagtga attcatcata acagagaggt cgccgccggt cgggttcttc atgcctaccg
     1561 ggatatccag accgctggca gtcagacgat gctgctggtt ctcaacggaa cgtgcgccga
     1621 tggcaacata agacagcagg tcagacagat aacggtggtt ttccggatag agcatctcgt
     1681 ctgcgcaggt cagaccagtc tgctcaattg cacgggtatg cagctcacga atggcgatca
     1741 gaccctggag catatcctct ttcttttccg gatccggctg gtgtaccatg cccttatagc
     1801 cttcgccggt ggtgcggggc ttgttggtgt agatacgagg gatcaggatc agcttatctg
     1861 ctaccttttc ctgtaccttt gccagacggt tcacgtagtc cagaactgcg tcctcacggt
     1921 cggcagagca aggtccgata atcagcagct ttttgtctga ttcgccggtg aataccttct
     1981 tgatctctgc gtcacgcttt tccttgaccg aaatcagctt ttctgaaagg gaaaactgcg
     2041 ctttcagttc cttcggaatg ggcagctttc tgataaactc catattcatt ctaaataagt
     2101 ccttctttct cattacatta gagcgcagca ttaggctctc aaaactgcgt cgccgcaaac
     2161 cgtttttcgc aatgaaaaat ctgtgcttgt ccgttcagcg cagcagtgct aaggtatagt
     2221 atactatatt tttccgaaaa ttgcaagccc tggccggaaa atcacgaaaa gtttactggt
     2281 gtcgctaccg gcggcgtggt gtctgtgccg gatgtttctt cttcctgtgc ctccggcatc
     2341 agaaattcca ctgtcagatt gccctgtgcc gtatatacgg tcacgatcgc ctgctgctgc
     2401 ggcaggatca taacgccggc gatctcagag gcagttagac ggcagtcatc ccactggtac
     2461 agcagctccg gctgcgtgtt ctttcccgcc atgcagtata gtccgtgttc gttccagata
     2521 ccgtagagga gactgtcctc ttcggattcc gccagcatgg catctgcggt cattcgctcc
     2581 ggcacagcgg tctgtgtttt ccacagtgca ccatccgtta ccacgatgtg agacaaatgc
     2641 tgcacatcgt cggtgtcaca ttcccacaaa agcatcccgt cgtcactgca ccgcacccac
     2701 tgataatccg attccaggga cagcacgttg tctgcatttc cctgcaccgg gcagaggttt
     2761 ccgttgtcat ccagtgccat caggcactcg gattcgtcga tcgttgccgt cagatagann
     2821 nnnnnnnnat agacatggtc agaggcatca cagcgcacgc cggtgaatgc catagtctgg
     2881 cgtgaagttt catccagatc cagcagtgtg gaaagtccca gttttttgca gaccacgccg
     2941 tttttatcgt accacaccag ctgatactgt gtggcataat tttcatagta ggattcccag
     3001 tcgaattcgt ccgggttaaa tgtggggtct tcatacggca cagcatctgt attttcacag
     3061 atcaagccgc agatcgttcc gtcagaaagt acatcgaaat ctgtcagccc gacaaaagca
     3121 gacgcattgg cattggcgta cagcggtgca aattccggtt ccgtatccgc ctttgcatga
     3181 tacaacacgc tttctgtctg ctgcaagtcg ttctgccgcg tcccgagcag gtagacatcg
     3241 tcctcataaa tgcgaacagt ctggagtgtt cccagctcct ccggcatttt ccatgcggca
     3301 gcttttgctt tatacaatgc ctcggcaggc gttgtctcgg taaccgagac cgtctcctct
     3361 ggctccgcat cagttgtttc ttccgaagta tccgtcacaa gcggcggaag atcggtggct
     3421 tttgtatgat ttgcagaggg attttgttct gttgcagcac agccggctgt acagaacagc
     3481 attcccatca ataagacgca cacggaatgt tttccatgaa attccattgc actgcctcca
     3541 ttcgtcaaaa tctttgtcta ttataatacg aatcgagtaa aatgtcaagg tgatactgca
     3601 caaaaacaga aaaaaccaat tcccnnnnnn nnnngctcag ctgcgccggg atgcagcggc
     3661 gattgcagcc tgcaaacgtg ctgtgatcgc cgcatctgta acgcacaggt ttttgtcgat
     3721 cccggttccc agatggatct ccggtttgat gaccggtccg tggaaatcag agccgcaggt
     3781 atacagcagg tcatatttct cggcgaggtg cagataaaac tgcgtctgtt ccggcgtatg
     3841 gcggctgtaa tatgcctcaa ttccctggag accgacgact ttcaatttgg caaccagtgc
     3901 atccagcgca tcattttcca gtttcagctg atgcggatgc gccagcactg cgacaccgcc
     3961 ggcgctgcgg atgacccgga tgccctcttc cggtgtgggt ttgggacgtt ctacacgggc
     4021 gtagaattcc ggtgtcgtca gatatttttc aaaggcatcc tgtaccgagg acgcatatcc
     4081 caactctacc agcgttctgg cgaaatgcgg tcgcccggag gttttcccct ggttgcagcg
     4141 gcgcacatct tccagcgtaa tggctgcgcc ttttttctgg aggtaggcaa gcattttgcc
     4201 gcagcgctct gtccggtcag cggcgtgctt ggcacagaac tggtgcagca catcgctgtc
     4261 gggatgaacg ccatatccca gaatatgcag ttcctttccg ccctgtacac tgatctcaat
     4321 gcccgggaaa aatgtcattc cacaggccac cgccgctgca cttgccgcaa caatgccgga
     4381 aaccgtgtca tgatctgtga ctgcggcaac ggtaacgccg gcttgatgcg ccatgcggat
     4441 ggtctcttcc ggtgaaaact gtccgtctga acaggtcgta tggatatgca ggtcgatcat
     4501 tttttctttc cctccgtaaa tacagccgct gtttcctgtt ccgcaaaagt atctgcatcg
     4561 cagagaatca gcacttgggt gtaatccgac acatctgcca gttccgaaag cgaatgctcc
     4621 atctctgtca catccagaat aca
//
LOCUS       DAWENO010000168         4624 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6854_length_4624_cov_12.027796, whole genome shotgun sequence.
ACCESSION   DAWENO010000168 DAWENO010000000
VERSION     DAWENO010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6854_length_4624_cov_12.027796"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1009 a         1237 c         1271 g         1107 t
ORIGIN      
        1 cggatccagc cgtagtcata ggcgtggttc atgatggtgt gcgccttgta gtccgtgctg
       61 gggaatccca ccagattctg ggagactgtg ccggcggtga aggagctgac gatctgcatg
      121 actgccgcaa acatcagctg cggtcccatg gacgggatgg taatgtagat cagttcctgc
      181 cagcggctct tgatgccttc gatggcgcct gcctcatact gcgagcggtc gatgttctgg
      241 aatccggcac ggagcgccag gaatccggcg cccaggctga tccacatctg caccacgatg
      301 gtgacgccca gcacatagct gccgtcggtc agccactgga tgggcgagct gatgatgccc
      361 agatccagga ggaacgagtt cagatagccg taggagtcgc cgctgaaaat cagtttccag
      421 acggtataga cgttcgccat ggacggcgca tagaagatca gagtaaagat ggtgcggagt
      481 ttcgggtgca gctcattgat gagccacgcc agcaggaaac acagcacata gctgatcgga
      541 ccggtgaaca gggcgaacac cagggtcaca cggatggact ggataaacac cttgtccgag
      601 aggaacaggg tggtatagtt ggacagtccc acaaatttcg gcatctgtac catgttgaaa
      661 tccgtaaagc ccatcggcag ggacatcacg accggtacga tggtgaatac caggaaaaac
      721 agcatgaatg gagcgatcat cagatagctc gccttgttct gcttgactgc cagccaggtg
      781 agccgccggt tttcctgtcg ggttcttttg ccaatctcgg cagtatccaa ggggattccc
      841 tccttttcgt taagtatcta atccgaggtt ctcacgcttt ctggtgatct cctcgttgat
      901 gtcccggttg tacgacatca gcgtatcacg gggattctcg ttgttgttga cgacgtcacg
      961 gaatgcgttc atcacatttc gggtgacgga ataggatgcc gggatgatcg ggatctcttc
     1021 cagttcatcc attgcaccca gaatggtgtt tgcctcatcg gtggaccatg ccagctgggt
     1081 gagcgcctcg gtgtttgccg ttgcgtaccg tcccatctgt cccagcagtc cctcgatctg
     1141 ggtgccgtat tctacctgga catcggtgga gcagaaccac ttgaggaacg tccacgcatt
     1201 ttccaggccg tttgtctttt tgtctacctt attaaagatg actgcgccgg tgttgttgga
     1261 gttgacagcg tgagaaatgg tgccgtcctc ccggagcgta cccggaatgg gagcaaatcc
     1321 ccacagtccg tcgatctccg gcgctgccac tgccagctgg ttgaagaagg tgcagtagtc
     1381 tgaaaccaca atgggatatt ccccggtacg gaaccggctg aacgcatcgt actgctggtc
     1441 gaagtcatac ttggtgtaga aatccgtcca catctcaaat gcgtcaatgg cggcgttggt
     1501 attaaaggta gtctccgtct gcgccgcatt ataatagttc tcgccacgct ggagcagcag
     1561 ggacgcaaag gtcagaccgg attccgtcgc cgcagagatg gtcgcctggt tggcatccgc
     1621 cgcaaccgca ggcagcacca gaccggcaga catatagttc cgctggagcg ccgggagcat
     1681 atcgatcaga tcttcccaag tctggggtgc ctgggtaaag cccagttcgc tgagaacgtc
     1741 cttgcggtag aacatcatcg cccagctccg ggtcagcggc agaccgtatg tgccgccttc
     1801 gtactgatac ggcaccatgg cgttttcctg gtacagcttt gtcatttcgt catagccgtc
     1861 cattttggaa acgtccgcca gcagtccacg ggcagccagg ttcaccggga attcgccgcc
     1921 caggaacagg gcaacgtccg gacctttatc cgccagtgtt gcctcgacta cgccgccgac
     1981 taccaggtta atggagatcg gcacgttgta ctgctgctgg aactccgatt ctaccaggtc
     2041 ttttaccacc tgcgcctggt cacgtcccag ggacacccag acttcgatgg catcgtcccc
     2101 tgtggtggag gacagcgagt tatagctttc gtcccaggat gccgtaaagc gctggaagct
     2161 ataggagagg gaactccaga atcccgcctt gatggacggg aaatccttgt ccggagacgc
     2221 caactccaga taatccactt ccagcggctg gtcacgatag tcacgcatcc aggaggaaag
     2281 ggatgtgata taatccttga tctgggacag ataattcggg atcttcagcg gatcgtcgac
     2341 gcacttgtcc aggatcacgg tcatctgttc cagcgacgcc gcctcagaac ccttggagtt
     2401 cgccagcgac tcaatgtgtc cctggatctc tttcagttcc ccggaaatgc gctggaattc
     2461 gtctaccagt tccgggatct tttcgtgtac ataataatcg gtatacttgt ccggctccgg
     2521 tccggtaatc atcacgatcc tccggtaata ggtgttcaga tccaggacaa tggcatccag
     2581 ctgacgcatg gagtcaccga tctcgccagg gatgacctcc atggtgatgg tgtgttcttc
     2641 ccctgctgtg aggtagacat acacatcctc gccgtccccg ttctggacgg tggtcatgcg
     2701 gaagtcggtg tcgtagtaga acttcacctg atcgaattcc tggctgggaa ctgtgccgtc
     2761 aatataaatg cggcggttgg agtaaaaacc acgcatttct tcctggcgtg ccttgatgcc
     2821 cagcttatac cagccgtctc ccggcagtgc gtttgcatcc accttccagg tgatggtctg
     2881 gagcgccttt tcccagttgc cgctgccgat ggtgttgtac accatgtgtt tcgggtcaga
     2941 gggcgagatg gcgctggtgg agttgtcata ggtcggatac agcacggaat ccgatttcag
     3001 cacggcgttt tcgccttcga tgcggacaac gccggagggt gtatcttcct tggacaccgc
     3061 agcattgacg ctgtcggtat attccgcata ggtgggaact tcgtccggct gtgcaaagcg
     3121 cagggattcc agggcgaagt atgccttctg ggaggaaagg gtgatctcat gggtcccctt
     3181 ttccagatag aagaacaccg ggtcattgaa cagaccgtcc gaatcgccga tccagctggt
     3241 ctgccacagt tcggtctgtt cctgtgtcgg acgcacctgg ttgccggaat cgtcggtctt
     3301 gatctccgac tgattctgat ataccttatt tagtttgaga cgggacgccg tatcataagg
     3361 cacttcgccg tcgattgcca gagacacaga gatctcggca gtattggaga cgatgggaca
     3421 ataggatgcc tgcacgcagt acaacccggt ctccggcaca ttcagcgtat agctgaagct
     3481 gccctctgcg ctgttccaga ccagcacatt ttcccggctc tcgccgttcc gatcggaata
     3541 gctgccggtc tcataaccgg tgcagtccgc cttctggaaa tctgcgccgc tcagcaggat
     3601 ctccgcctcg gggcgtggtt cgtcccgata ggtgtcgtaa tagtcgctgt aggtctcctg
     3661 ttcctcatag gaaaggtcgc cgccggtatc cgttccggtc tcttccgcag ataccgccgt
     3721 ggtctgcgct gtgtctgtgc ttggtgcctt tgccgcaaat gctgtggaca ggggcagcgc
     3781 cgccgccgtc atcagacaag ccagcacggc tgctgcggca cggctgtgcc atttgtagtt
     3841 cgccaaggct cttccaccct tctttctgtc tgctgcgatg acagacgtat tccggagcgt
     3901 gtctccggaa aaatacactg cacgtctttt cgggatgcca cactctaacc ttctgtgcaa
     3961 gctgcacaga tcgtcacttt ttctgcggat gaccgcatct ccagcggcgc acccttcggg
     4021 aattctacaa attatacaga aatcccgcaa aacagttcct aaatattata gcaatttccg
     4081 gtgaaaatgt caagcgagtg aaaatccggg gcggattttt gttcattttg tgaaattcgg
     4141 aaaaaacggc gaaattgcgt ggattttccg gtgctttccc acaatttctg cacactttca
     4201 cggatttagc cgcaaaaaaa ttttatggaa acacatggaa gttacagatt gtgcaagctg
     4261 ccggcgcaga aacgaacgag tttttgagcc ttttcacggg aaaatacacg caaaaaatcc
     4321 ggtctgcccc ttgacaaact ggatggaaac gggtataatg aaaatagaaa gagcataccg
     4381 gcagacggtt gctcccatga aataggtaaa taagaattaa ccgctttcag tttggtcgca
     4441 gagggcggtt atttcttttt gtgattatca tggaaaattg tatataacaa ggatgcgaat
     4501 gcaatcaaaa gtgtcaggag cgctaaaagc tctgaccatg ttacgtaatg ttccattgta
     4561 tcacctccca tcgtatcaaa tacatgggag ctggaaatca aaccgtccac cgttttcggt
     4621 atgc
//
LOCUS       DAWENO010000169         4592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6903_length_4592_cov_12.897730, whole genome shotgun sequence.
ACCESSION   DAWENO010000169 DAWENO010000000
VERSION     DAWENO010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6903_length_4592_cov_12.897730"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1065 a         1235 c         1334 g          958 t
ORIGIN      
        1 ccttcctgca atatgcaatg tcggtcattg ttgcccgtgc attgccggac gtgcgtgacg
       61 gtctgaaacc ggtacaccgc cgtattctct ataccatgtt cgaaaagggg ctgacacccg
      121 ataaggcata ccataagtgc gccgataccg tcggtgctgt gctgggtagc tatcaccccc
      181 acggtgacgc ttcggtctat gatgcgctgg tgcgtctggc gcagaatttc tccatgcggt
      241 atatgctggt ggacggtcag ggtaacttcg gttcggtaga cggcgacccg cctgctgctt
      301 accgttatac agaggcaaga atgtccaaga tcgccctgga aatgctgacg gatatccaca
      361 aggagaccgt gcctttcgtc cccaactatg acgaccgtct gaaagaaccg gcagtgttgc
      421 cgagccgttt tcccaatatc ctggtcaacg gctcggtggg tatcgctgtc ggcatggcga
      481 ccaatattcc gcctcataat ctgggcgaag tcatcgacgg catccatatg ctgatggaaa
      541 atccggactg caccatggat gagctgatga cctgtatcca gggaccggac ttccccaccg
      601 gcggcattat catgggcaag agcggcatcc gtgccgccta cggcaccggc agaggcagga
      661 tcaccctccg tggacagacc cactttgaaa aggtgcgtgg acgtcagtcc attgtcatta
      721 cggagatccc gtacatggtg aacaaggcac gcctggtgga gtccatcgcc aacctggcaa
      781 aggataagcg catcgagggc attcaccaca tccaggatga gtccagccgt gagggtatgc
      841 gcatcgtggt ggaactgaaa aaggatgcca acgaacagat cgtgctgaac aagctgttct
      901 cctataccca gctccaggat actgtcagcg tcaatatgct ggcactggta aacggcgagc
      961 caaagacatt gactctgaaa caggttctcc aggagtatct gcagttccag gtggacgtta
     1021 tcaccaaccg cagcaaattc gatctggaaa aggcaaagaa acgggcgcac attctccagg
     1081 gtttcctgct tgccatcgac tatattgacg aagtgatcgc cattctccgg gacagcaaga
     1141 ccgtgccgga aggtaaggct cgtctgatgg agcggttcaa ggaaattgat atgtctgccc
     1201 tgctggatcg tgcgcagtac gatgtggacg gctatcagct ggaacacaag cagggactca
     1261 ccgaagagca ggcagaggcg attgtgcaga tgcgcctggg acagctcacc ggcatggagc
     1321 gccagaaggt caccgacgaa ctgtacggac tgatccagaa gatcactgac ctgctggata
     1381 tcctggcaaa ccgggaacgg gtataccaga tcatcatgga cgatctgaac gagatccgcc
     1441 ggaagttcgg cgatgcacgc cggacgcaga tcgagatggt agacggcgaa gtggacatcg
     1501 aggatctgat cccggtggag gactgcgttg ttacctatac cgaaaacggc tatatcaagc
     1561 gcatcccggc agatgtctat aagagccagc gccgtggcgg ccgtggcgtt accggcatga
     1621 aacagcggga agaggacttc gtcagcgagc tgatgatcag ttcctctcac gcccacattc
     1681 tgtttatcag caatctgggt actatgtaca agctgaagtg ctacgaactg ccggagggtt
     1741 ccaagaatgc ccgtggcacc aacatcgtaa acctgctgcc cctgtcacag ggcgaaaaga
     1801 tcgcagccgt tctcaccacc ccggacttcg aagagggcaa gtatgtggtc atgatcacac
     1861 gttcgggcaa gatcaagcgg acaccgcttt cggcttacaa gaacgtgcgc aaaaacggct
     1921 tgattgccat tggtctggac gagggcgacg agatcgccgc agtccgtctc accaacggtt
     1981 ccaaccggct gtttgttgcc acccggaacg gcatggcgat ccgcattgac gaaaacgatg
     2041 tgcgtcccat gtcccgttcg gcacacggcg tcaaggcaat caccctgcgc aagggcgatg
     2101 ctgtggtatc catggcgaga gagcgtgagg gcgcatccct gctgaccgtg accgaaaacg
     2161 gatttggcaa aaagacggcg ctttcggaat accggatgca gtcccgtggc ggcatgggtc
     2221 tgaccaacta caaggtggac gaggaatacg gcgcagtctg cggcatcaag gtagtagacg
     2281 agaccgacga cctgatgttc atttccagcg acggcgttat cattcgtgtg gcttgcagcg
     2341 agatccgtga aatgggacgt actgcaaagg gcgtgcgtgt catgcgtgtc agcgagggtc
     2401 agaaggtagt tgccttcacc cgtaccgagg catcccagga agaggatgac gcagaaggcg
     2461 ctccagatgg cgctgaacag ccttccgcag agccggagtc aattcctgca ccggatgcac
     2521 agaatgcgtc agagaccggc acagaggctc ctgcggacga atctgcggag taacgtgcag
     2581 acattgcaat aaaaatcagc agcggcggag attctgccgc tgttgttgta tggagaggac
     2641 agtgcatgat aaggaagagg agttgattgc ctcccttgaa aagggtgatt ccccacgggg
     2701 tggggaaatg tcaccgtagg tgacaaaggg gaccgctcag tacgaggtgg atgctccgtt
     2761 aaagagcaga cggagggata aatccccctg tcagcttacg ctgacatccc agcacaaggc
     2821 acaccctacg ggcgcctttt caagggggac tatcttaaaa attggtttgt ttgcttgaaa
     2881 aaattttacc gtaggggcga acatcgttcg cccgagactc caccgtcaag cctgtggctt
     2941 gccacctcct ctacagagga ggcttattaa acaaaaatcc cccggagggg ttacaggcag
     3001 cttgtctgcc gtgacattac ccctttttga acgttaaatt ggggcgaaag gtcagcggcg
     3061 acccgccgca cacgaggatt ttacttcaca tgattttttt gataattcta tttgttttga
     3121 tcttactgat tttcatttgg tttctcatcg aacagcacct gctttgggtg catcgggagg
     3181 aaatcacact ttcccatctt cccccggcat ttgacggcac atcgttgctc cagatcaccg
     3241 acctccacca ccgggaaatg ggaaaaaaca actgccgcat cgtccgaaaa gcggcagaac
     3301 tgcaccccga tcatatcgtc ctcaccggcg atctgatctc acgggatatg cgtgactttc
     3361 acggaattgc gtcgtttttg actgcacttt ccaaaataag cccggtctgg atctgcgtgg
     3421 gcaaccatga gctggatctg ccgccggcgg tctgggaaca gctgcggcag accattgcgc
     3481 agagcggctg ccggctgctg caaaaccaga ccatttccct ggaaatgccc ggcagtgtgc
     3541 ctctgtatct ggcaggcgca gacctggcgt atggggtgta ccgggacgag aaccggaagt
     3601 tccgcaatct gcagccctat acggcggcag acctggaaaa cgacctgggc aggcgccggg
     3661 gctgtacggt gctgctggca cataatcccc tgctgctgga cagctacacc ggctggggcg
     3721 cagacctggt gctgtgcggt catgtccacg gcggtctggt gcgtctgcca tttctgggcg
     3781 gcgtgctgtc gccggagcgc aagttctttc ccaaatacga caaggggctg tatgaaaaag
     3841 acggcacgaa aatgtatgtc tccggcggca tcggcaagtc tcggctgtgc aatccgccgg
     3901 agatcagtgt gattcacttg aaaagcgtcc agaaataata gagcagcccg tacaccatgg
     3961 atgcggtggt gccgtagagg atgaccggcc cggcgatggc aaaaatgcgt gtgccgatgc
     4021 ccaggatcca gccctcggtg cgtgcctcga tggcggagga aaccacgccg ttggcaaagc
     4081 ctgtgatggg gacgagcgtt cctgcgccgg cgtgcttgcc cagcttggcg taccagccca
     4141 gcccggtgca gacggcgctg actgccacca gcccgatgga gacccacgca gaggcgtttt
     4201 tttgttccat gccgcagtat tccgtgagaa tatggatgcc tgcctcgccg atggtgcaga
     4261 tcagtccgcc gatcagaaac gccaggggaa tgttcttcca ggacttcgac cggggtgcgg
     4321 tctgatcctg catggttttg taaagttcac gactgatttt catatgcagc cctcctttgc
     4381 agaaaagtat gtgcagtttt ccggggatta taccggaatg tttcacgtga aacattttga
     4441 aacggattgc tgttttacag agaaggcata tcgtaggggc gaacatggtt cgcccgagaa
     4501 cctctgactc ctccgtcaag ccagcagctt gccacctcct ctatgagagg aggctttgcg
     4561 aaattgtctc ctctttagag gtgattcccc ac
//
LOCUS       DAWENO010000170         4575 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_6931_length_4575_cov_11.583407, whole genome shotgun sequence.
ACCESSION   DAWENO010000170 DAWENO010000000
VERSION     DAWENO010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6931_length_4575_cov_11.583407"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1993..2002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          970 a         1284 c         1316 g          995 t
ORIGIN      
        1 aggcacaaag ccacagtaga gcgtctgacg gaattggaat tccgcacatt tgacaaggtg
       61 cagggcatcg gcggacgggc tgcctgccag gacgactggg agaccttttc catcatgcgc
      121 cgcagccagt atctgccctg ggaagaggaa ctgctggagc agtggcttgc ggcattccag
      181 gcggcggcag atgccggacg caatctcatt atggaaaaat atgcccggat gatggaaagc
      241 acagacccgg cagaatatgc cgcctttgca gaccagcttc cggcgctttc gccggaattt
      301 gtccagctcc gggaggcggt catcgccatt cagatcccgt ggatggagga atttgcggcg
      361 cagtatccca atctggcatc ccgtgccaga gtcattcaca cggcagaaga ttccccggcg
      421 cagacctcct atgagaccta tctccggggc gagctttccg tgtatccctt tgagatcctg
      481 tacggttacg gcagatgggt ggtatccctg taccagtccg gtgaaaatct cacgtgcctg
      541 actatggcgg agaccgtccg tgcgtatgga tacggctcac tggaggacgc agaaaaagcc
      601 tgcggagaaa caccgctcag cggcattcca ctgcatctct gagccacgac agaaaaggag
      661 aaccgacatg aaaaaaagac agcgtttcgc cgctttgctc cttgcggcag ggatgaccgt
      721 ttccgttgcc gcacccctgg gcgcagcggc ggagactacg gacaagacag ccgacatgac
      781 cgcattctac caggaatgga agtcgaaata tgtgcggcag aatccctatg tgacagacga
      841 gacccagtac tatgtgtact acagcgagga tacctacagc ggcggcgagc ctgtgccggt
      901 gacagtttcc gaggcgcacg gctacgggat gctgatcgcc gtttccatgg cggactatga
      961 cccggatgcc agagcaattt ttgacggcat ggtgcggtat taccaggcgc accccagcga
     1021 gatcggattg catctgatgg cgtggcagca gagcgacaac ggaaagtctc tgacggagac
     1081 ggacggcgca gactctgcta cggacggaga catggacatc gcctatgccc tgctcctggc
     1141 ggacaaggtt tggggcagcg gcggcagcta cgactacgcc gccatgggaa aggctgtgat
     1201 cggggatatc atgacctatg aagtgaacca ggagacctgg acgctgagcc tgggcgactg
     1261 gacttatggg gagagctccg acagcaaata ctacggtgcg acccgggcat ccgactttat
     1321 cctgcagtat ctgccggtgt ttgcggcggt ctccggggac gaccgctgga caaaagtcta
     1381 tgacagcacc aacgccatca ttgacagcat ggtggacaaa tacggcacgg gactgctccc
     1441 ggactttctc attcccgacg gcaaaggcgg ttaccagccg gcgccggaaa actatctgga
     1501 ggatgtgacc gacggacagt acggctataa cagctgccgt atcccgtggc gcatcggcat
     1561 ggatgcggag aacaatccgg cggcgagaaa gtgcgtggat gcgctgaacc ggttcattca
     1621 gaaacagacc ggcggcgacc cgtgggagat catggcaggc tatacgctgg agggaaagcc
     1681 caccgcagac tataacgacc tctgtttcac cgcaccgttc ctggtggcgg cgaagtacag
     1741 cgaggatgca gactggaacg aacagctccg ggactgcgtg gtgaattacg gggacgacgt
     1801 ctacttcgga gacaccatca aaatgctgtg tctgatcgtg gatgccgatg cctggatggt
     1861 gccggagacc ggggagactg agacaaaggg cgatgtgaac ctggacggca gcgtcaccat
     1921 gggcgacatg gtgctgctgc ttcggcatct gctcggcagc gagacgctga cccaggcgca
     1981 ggcggcacag gcnnnnnnnn nnatttattt gtatgagtac taagtgaaaa tcaaactgtt
     2041 tgtgcagtta aagtcaagcc aagcggtttc ataatcttta ccagagtttc aaggttggga
     2101 acggttttgc aggactcaat tcttgcaata gaggactgcg gcatatgaca catatcagca
     2161 agttcccttt gagaatatcc taaagcgttg cgctgttcaa tcacagcaga gataatagct
     2221 gccacggttt ccatttcttc cacatatgct tttccctgcg gactggttgt tttaacgtgt
     2281 cctttatagt cattccatgt cctcatagat catttcccct ttctgaataa ataatcatct
     2341 cgctcagact ttgctttttc aatgtcttag cgcttacatc gacactgtgg cttgtgcggt
     2401 attgcttacc ccttcgacgg catcaccttt gccagagcgc ctgccagacc ggacagcggc
     2461 agggtctgga gccagatacg tcccggaccg gtgaccttgg tgttgaacag cccttcgccg
     2521 cccagcagca cattgcccac gcccttgacc atttccacat ccagggtgca ggtcgcctcc
     2581 atggctgcca ggttgccggt gtccaccatc atggtctgtc ccggtgccag gtcatatgcc
     2641 accacgctgc cgtcgatctc caggaacagc ataccgctgc cggagaattt ctgcatgata
     2701 aagccctcgc cgccgaaaaa gccgccggca attctcttct ggaagaacag ctcaaaattg
     2761 acggtggatt ccgacgccag aaacgccgac ttctgcgcaa tgatggagcg ctgcggcgtg
     2821 atctggatgg gcatgatctc acccggcaca gcggatgcaa aggcgatcat gccctgaccg
     2881 cctttggcgg tgtagtggtt ctggaacatc gtctcgccgg taaacgcacg gctgaacatc
     2941 ttgcccagtc cgccgccttt ggtctgcatc tccatgttgg gcgtcatcca tgccattgcc
     3001 cctttctggc acagaatggt ctcttccggg tcggcggtca caacgaccac cggatagggc
     3061 tgtccctgaa tctgatactg cataataaca tcctcctggt tttggcgtat tcccgtttgc
     3121 gggaacacgg ttttgtgtgt ttaggcggtg tcgcctttat ccctgcaatc cctttgcgcc
     3181 gatgtgtacc tctccggcgt ggagcgtttc cacattgccg aaactgtccc gtaccactaa
     3241 gccgcagtca tcggcaattt ccagcacggc gacctttttg acagtgccgt catcctttgc
     3301 cagctccacg gtctgcccga tgagacagga acggctgcgg tattccgtca gaaaacgccg
     3361 ctccggaagt ccctggagcg cctgctccat ctcgtacagc accgccgcca gcagctgcgc
     3421 ccgacagacg tgggtgtccg gcatggattc ttccagtgaa gtcaccgtgt gccgcagttc
     3481 ctccggcagg gcgatgctgg tgttccggat gttgatgccg atgccgatga tggcgtgttc
     3541 cagcttgccg gattccaggc tcacgccgcc ttcggtgaga atgccgcaga actttttccc
     3601 gtgcagatac aggtcgttta cccacttgat ctgcacctgt gtgctgtaaa gggcatcaat
     3661 ggctctggcg gcagcgacgg cggcgcaggc agtcagcagc ggcgcatcct gcagagagac
     3721 cgccttggac aggatcatag agaagtacag tcccgatccg gcaggggaca caaaagtgcg
     3781 gccctgtctg ccttttccgg cagtctggca gttggcggca accactgtgc cgtgctttgc
     3841 cccgtcccgc accagcgcct tgcaggtgtt gttggtggag ggaagttccg ggtagacata
     3901 caggggatgt cccaggatgc ggctgtggag atagggacgg acggtctctt ccgtcagcag
     3961 gtctatgccc tgtgccagcc gatagccggt ctttttcttt gcctcgatgg gaaagccctc
     4021 ttcttgtaac aggcggacag ctttccacac agcgtttcgg ctcacgccca gcttttgcgc
     4081 cagggtctcg ccggagagat actgtccggc ggcctccgtc agacaagtag caacggcatc
     4141 ttttacacgc atggtctgtc atgctccttt tcaaattttg tggatttttg gatattttta
     4201 ttatatagga ttttcatgat gttgtcaaga aattgtttgc ggcagaaggc gttttcctat
     4261 aaccgtaggg gcgaacatcg ttcgcccggg actcctctga ctccaccggc actgcgtgcc
     4321 acctcgtact gagcggtccc ctttgtcacc tacggtgaca tttccccacc ccgtggggaa
     4381 tcacctctag aagaggaggc tttgcgaaaa tgtctcctct tttagaggag atgcccgata
     4441 gggcagagga gtcttttgcc tcccttgaaa agggaggtgg atgctccgta aggagcagac
     4501 ggagggataa tccccctgtc agctaacgct gacatcccag cacaaggcac accctacggg
     4561 cgccttttca agggg
//
LOCUS       DAWENO010000171         4524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7028_length_4524_cov_10.927948, whole genome shotgun sequence.
ACCESSION   DAWENO010000171 DAWENO010000000
VERSION     DAWENO010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_4524_cov_10.927948"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1267 a         1180 c         1147 g          930 t
ORIGIN      
        1 acacgactaa gacgctgcgt ttgcacgatc tgtgcagccg ctgacaaaac agcacttcac
       61 cccgaaagcc ggttttccgg tttccggggt gattttttat aggaaagcgc ccggcggcgc
      121 ataaacattc tgtacaattc tatgaaacac gatttgtgca ttttgacggt ttgtactaaa
      181 accaccccag tgattggcga aaatctcttg acagcttgca caaaaggtgg tagaatgagg
      241 tggataaggt gatgccgcag cggcagcaga gggctgtcgg ctgcgccgca cgaaaaacga
      301 aggagttcga taacatgaag tttatgaaga aactgctcag caccatgacg gctgcagcaa
      361 tcgggctgtc catgaccact gcactgtctc tgggtacttc tgttgcatct gcagcagaca
      421 agaccgctgt ggaactggtc gaggatatgg ggctgggctg gaacctgggc aacgccctgg
      481 attccaccaa cacctgggac agctctctga cacccagcaa gatcgagacc gcctggggca
      541 acgtggttac cacggagaac atgattaagg aagtcaagaa ggctggcttc aacaccgtcc
      601 gtatccctgt cacctggtgg gatatgaccg gaaagtccgg cgcagcaaat atgaacagct
      661 ttgacggcac ggtaggcgat gcgtacctgg caagagtcaa ggaagtggta gactactgca
      721 tcgacaacga tatgtatgca gtcatcaaca cccaccacga cgaggactgg gaaaaggata
      781 cctccaagat cggtaccttt gaaaagctgt ggacacagat tgctacttat ttcgagggct
      841 atgacgagca tctggtattc gagggcatga acgaagtcaa cttctctacc agcgatgcca
      901 tgacctataa ccaggcattt gtagataccg tccgtgcaac cggcggcaac aacaaggatc
      961 gcctgctgat ctgcccggca gacaacaaca acaccgcaaa ggcactgtcc agcagcttct
     1021 ccatgccgaa ggacgactcc aatatgctgg cagtttccgt acactattat gaaccgtcta
     1081 ccttctgcgt agctgatacc acttcttcct ggggtcaccg ggagacatgg ggtactgccg
     1141 cagatctgac caaggtagaa aaggacttcg gcgaactgaa gtccaagttc accgataacg
     1201 gctacggcgt tgtcatcggc gaatacggcg tctgcaatgc cgacaagtac ggcgtaaaca
     1261 agaccgccta caacaaggac gaagagtcca tcaagacttt ctatcagacc atcacttcca
     1321 ctgcgtacaa gatggacggc atctgcccca tcgcatggga tgactccgac tccggcacga
     1381 tctgcctgtt cagccgcaag aacatgagct ggtatgacga aagcctgaag agcatcttca
     1441 ccgacattgc aagcggcgga aatgtagatc cgggcaagca gaagaccgac cgtgtcacat
     1501 tccaggcaag cgacatcacc gaaaccgata aggacggcaa gccatatctg aatgtggatc
     1561 tgaagccgta caaggaactg ggcgtacacg taagctctgt tgtcgtagat tataagatga
     1621 cctctgcaaa gaactcctcc gagtgcagcg gcgacatcaa catgagcttt aacgtgactg
     1681 atgaaaacgg cgaaagccag tgggcatacc tggacaacag catcggaccg aaggaaacca
     1741 cttctacctt tgatgttccc acaggttctc acgaatatgt tctggaaaag gacgacgacg
     1801 gcaatgttac cagcagtgtg actggtactc tggagatgga ttatctgaag ctccagaact
     1861 ggtggacatg gtccggcgca accggtgaca aggtgaccgt cgatatgagc tctgttaccg
     1921 tgatcttcga tgactacttc tatgtagacg acaatccggg ccagaccacc gagaccacac
     1981 agaccaccac cgcaacaacc acttctgaaa cctccactac cactgtcaca tccagcgtac
     2041 cggagggtga cgcaatcggt aaggcatact tcatcggcgg cctgggcgca gaccagaact
     2101 ggactgccgg cgaaaacgaa ggcgcagcaa tcacacccat caccggcgac ggcacttata
     2161 ccgtttcctg ggatgttacc ggaggcggcg gtacgaatac cgtacagttc ctggcagttg
     2221 tgatcgctcc ggacggcgaa accgaaaact tcaccactga tacattcgaa gacctcaagg
     2281 tttctctgga tgaagtatgg atcgacggcg aaaagctggc agattacacc gttggcgcaa
     2341 agtccatcaa cacacgctac tatgaagaag gctctgccgg cgtaacacgt ctgtacctgc
     2401 acgatgactg gtccaccaag atcaaggatc tgccgggcaa gacaaccatt acccaggaag
     2461 tcaaggtagt attcaccatt tccggtactg gccacgaagg cccgatcgta actaccacaa
     2521 gcgaacagcc gacgactacg acaacatctg caactacaac aaccaccact actacaacta
     2581 ccacaacaac gactaccacc accacaacaa caaccactac cacaccggag ccgaccacga
     2641 caaccactgc atccaccaca actactccgg aaaccacaac ttctacaact aatgtggaat
     2701 ccactactac aacacctgca cctaccactg caaccaccga gacaactacc acaacatctg
     2761 caaccacaac caccacgaca accaccacaa cgacgactac cactacgacc accaccacca
     2821 caacaacaac taccacaacg atcacattca tctccaacga cattcgtccg gctaccatct
     2881 tcggtgatgt gaaccaggac ggcagcatca acatctgcga cgtcgttctg ctgaacaaga
     2941 tcctttccgg acaggtcagc gcaaacgagg cacagcgtgc aaatgcagac gtgaatgcag
     3001 acggcgctgt ggacggcacc gactctgtga ttctggtgaa gttcctggtt cgtctgatcg
     3061 tggctctgcc gaacaacgat attgcttaaa caacaggtga ctttcctgta aaaagacagc
     3121 ggggctttgc cctgtaatat gtaaaataag ctgaaaaaac gtcgtgtctg tgaatgcaga
     3181 tacggcgttt ttttgcaggc acaagaaaaa aaggtttcgc aaagtaaaat ttgtgaaaac
     3241 tgctggaatc ggcggaaatt ccggcaaaat acattgtaaa aatacgtttc tgcttaaaaa
     3301 tcggcagtcc ggggagacga atacttatca tttttctgga aagtgtcgcc gatttgtgaa
     3361 atgtgcaaca aaagtagaaa agaaacaagc ggaaatcgtc atgaaaatag attgactttt
     3421 tgaaaaaatc gtcctataat aaagaaaacg gtgctaaaca cactgttgat tttccgcatt
     3481 tccggcagat tggaaaagag aggaaaacct gcggaattcc gcagaaaaat tcctttttca
     3541 gatgccggaa aacggaacag gaaaaggtgg tggatgcaat gggtgaagaa ctgcaggaac
     3601 agctgcaaaa caagatcctc tgggagagtc cctttgatgt gccgctgctc ggaccgatcc
     3661 acatcagcga gtctgttgtg atgacctggt tcattatggc gatcatcatg gtgctggtac
     3721 tgattttgac acgagatctc aagatcgttc ccggaaaacg gcaggcgttc ctggaaatgg
     3781 cagtcacatg gctcaccgat ttctttgtgg gcaacatggg caaagccgga aagcgctatg
     3841 taccgtacct cagcacggtg attatctatc tgggtatcgc caatatgatc ggtatgttcg
     3901 gtttcggcat gaaaccgccc acgaaggaca tcaatgtcac ggtggcgctc gcagtcatga
     3961 gcatcgtact gatcgaggtc tccacattcc gggcgcaggg cggtatcaag ggtttcttga
     4021 aatcctttac caagccgatg gcgcttttga cgccgctgaa tgtgatggag cttgcgatcc
     4081 gtccgctgtc gctgtgtatg cgactgtttg gtaatgtgct gggtgcattc gttgtcatgc
     4141 agctgatcga acacgtctgc ggactgatcg tgccgatggt gttcagtatg tacttcgatg
     4201 tgttcgatgg actgatccag gcatacgttt tcgtattcct gacatcactg ttcatggcag
     4261 agggcatgga agtcgaagaa gaggaaaaag tgccgaaatt gcagagagca taaggacaat
     4321 gccgcacagg gcttgtgcgg tgcagtcttg aattcggcag ccacagaaag gagcgttccg
     4381 ctatgatcgg agcaggtatt gcagtattga caggtatcgg tgcaggtttt ggtatcggca
     4441 tcgcaacatc caaggcggca gaggccatcg caagacagcc ggaggctgcc ggtgcgatca
     4501 acaaggcgct gctgctgggc tgtg
//
LOCUS       DAWENO010000172         4504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7069_length_4504_cov_10.036188, whole genome shotgun sequence.
ACCESSION   DAWENO010000172 DAWENO010000000
VERSION     DAWENO010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7069_length_4504_cov_10.036188"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1171..1180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1262 a          979 c         1208 g         1045 t
ORIGIN      
        1 gatcatcggc agtcagagcg atatcctttc cagtcatatt cactgttcca attccgtttt
       61 gaacgctgtg ataaatgaaa agtttacggc ggcggaaaag aaaggaattg aaactacctg
      121 taagattctg atcacgatac cggagtacct ggaatatgac ctgagcattt tgctttccaa
      181 tttgctggac aatgcaattg aggcgtgtgc caaagaggaa tctcccacac agatcatgat
      241 gctgataaag aaaacacatg gatattaccg cattgtggtg aagaatacga ttcgggaatc
      301 cgttttgcaa aagaacaggg aattgaaatc ggacaaagaa aacaaggcac tgcacggctg
      361 gggactgcgt tctgtcgctg aaattgccga aaggcatctc ggagggctgg atatctacga
      421 gaaaaacgga atgttcgtgg taagtgtctt attagcaaat gatgaaatcg ggaacttggg
      481 gaagagacgg tgggggcaaa ataattgact tcctatataa aatgcgatat tctttttaca
      541 tggatggtga gcgaaatgat cgctgaactt agtaaaagaa tgtcgctttt tttatgcaaa
      601 aaggaaatcg tcgagcagga aatggcagag gtctatcagt atgggtttga aatcattttt
      661 tccacactga tcggtttctt tatcaccatt gcgatcggcg ctgtttttcg aatgtttctg
      721 gtttcactgg tgtattattt tgcatttgtt gcactgcgcc agtttaccgg cggatatcat
      781 gcgaacacat atttgaagtg caacttgatt tttgctgcat cgacgacgtt tattttcggg
      841 atggcaaaga ttgtagcata tggaacttat cccattagtt ttcatgtact gctgctgttt
      901 ttttctcttg tagcaatatg gttcaaaact ccggtggaaa atgaaaataa gccgctgtcg
      961 gaagaacaga aaatctgcaa tcacagaatc agtatgatcg tggctgtgat cttttccggg
     1021 atcagctgct ttgcctatca gaaatgggta ttgatatcgg caacaattgc ccttacgctg
     1081 tttgtcatag cgttaatggc agtcatagca aatccaagcc gagaggagga aaacgagtca
     1141 tgaaaaaagt tcagaagtct atgctggaca nnnnnnnnnn agctggcatc tgtcagcaag
     1201 tttgcagcag taaaggcagc aggttccgtt tcctgcgctg gtttccatca gccgaaagaa
     1261 ccgaaagcac tgcagaatct gaaaaagtaa caaacagcac tgcaccgggc gagcaatcgc
     1321 tctgggtgcg gcaatgttga aacattttca cccaaatctt cccaaatacg gcatacgaaa
     1381 gtgtgccgcc tgaatttcat gcacggaatc gtgctgcgat agaacatatg gaacctgtgg
     1441 ctttgtgaaa ggagtttttt gtggagcata gatcagactg cgaacaactg attgcagacc
     1501 ttgtagagaa tcattttggc atcgaaaagc atctgaaagg atgcacttat ttgaaagagg
     1561 cgatcgagat tgcagtgcag aatcccatcg tttctatgat gaaactctat gagctggtcg
     1621 gactgcggca tgggtgcaac gcagaaagcg tggagcatct gatccgctac gccatcgaaa
     1681 gcgcctacac caaaaacaag atgaaccact atttcccgga agtctacggg agaccgtcga
     1741 attccatggc gattcgccag gcggcggagt ttgtccggac atccgaaacg taacatttta
     1801 catttgcaaa attgacgaaa ccatatggaa aagcacctgt ttgatggaaa catcgggcag
     1861 gtgctttctc tgtcagaaat aaaaaaggaa aaaagtagaa aattaatttt tttgaaactt
     1921 tataatcgtt tcgctttcag gtaaaaaata gtataataaa agtagagaaa aatgttattt
     1981 tgtcaaaacg actgcgaaaa aggcagacag gtgccgggaa gtcatggagg tatgggtgcg
     2041 gtatgattcg ggatatttta acaaccatcg gggaacattt ctggctcatt ctgattctgc
     2101 tggcagaact cctgtttgca gtggtgctgg tttgcagcgg ctttcggcga aaaagcggat
     2161 gcggcaaaga gaaaaaatcg gaactcccca tgacgcagtt ttcgtttcac gatgtgcagg
     2221 aggctgtctt gatactgcgg acagacgacc agctgccggt gggtgcgtcc gataatttcg
     2281 agacgctgtt cggcacatcc tatgcggcgc tgtgtgcaga tgtttccctg ctgaatcagc
     2341 tggacgtaaa ccagaaactg tttcagcagt accggaagtg ggacggcacg gcggcatttt
     2401 cctatgactt tcagctgccg gggcaggaac gctggttttg tctgaatgtg atacgggaca
     2461 aggctgcccg gcgtgacctg tttttgttcc atgagatcac aacctataaa aacgcctgct
     2521 ttgccttgga agagcagatg cggaaaatcg agaaggagag ctttacgaag tcgcagtttc
     2581 tctcccggat gtcccacgag attcggacac ccatgaacgg catcatcgga atgctttctc
     2641 tgacgaagaa acagtgcagc cagaatccga aagcgctgga atatctgggc gaggcggaaa
     2701 acctgtctca gtatctgctg tcgatcatca acgatatcct ggatctgtcc cgtatcgagg
     2761 caggaaagct ggaactggaa gaaaagccgc tggatttgcg cagtattgcc aacaagctgc
     2821 gcaatatgtt ccaggagacc gtggaggcga aaaacgtccg gttctccgtg acgttccagg
     2881 attttgacac gtactatgtg attgcagacg atattcgcct ttgccaggtg ctgacgaatc
     2941 tggtttccaa tgcggtcaag tttactgcaa aaggcgagat cacagtgacc tttcagaaaa
     3001 tgcttcagga aaaggaacgc atggactttc tgattaaagt gcatgatacc ggcatcggca
     3061 ttgagccggc gtttctggaa aagattttcc tgccctttga gcaggagagc cgcaatatca
     3121 ccaagaagta cggcggcagc ggtctgggca tggcaattac agacaacatc gtccgcatta
     3181 tgggcggaac gatcgtcatt aatagtatgc ccggaaacgg ctcggacttt aacgtgttcc
     3241 tgtccctgcc gctggcaaca gaggagcaga tccggcagat tcctgtggaa gagatgccgg
     3301 aggaggtaca ggaaagtgcg gactatacct ttgcagggaa gcagattctt ctggcggaag
     3361 acaacgagat caatgcggag atcgccgtgg acatcctgga ggaggagggt gcatccgtcg
     3421 atgtcgccgg aaacggaaag attgccgtgg agatgttcga aaacagtccg gtaaatcact
     3481 acgatttgat tttgatggat gtgcagatgc cggagcgaaa cggcagagag gcggcaagcg
     3541 atatccgccg cctggacaga gcggacgcaa aaacggttct gatctttgcc ctttctgcgg
     3601 atgcctatgt ggaagacaag cggctgtccc gctcttacgg catggacgga cattttgcaa
     3661 agccggtgga ttttcagaag atgaaaaccg agatcggcga aatcatccgg cagaaaaagc
     3721 ggtaagtgtt tcggactttg cacagaaagt ccgtggaggt gggaaaaatg aaaaaagtgc
     3781 agaaacggat tctgaccgtg atcgtttcgc tgacaattac gggttccggc atcgctacct
     3841 atctgcggta ttcggggaat tcctttgatc cggaaaaata cacccgaaac tcggcttgga
     3901 accggaatca gatcacgttt gacgacgatc aaacagacga tgaatacatg aacagtgaaa
     3961 agcaggatga taccacagag gacagccgcc agctggaaca ggatgacaat gctgaccagg
     4021 cgcagacgcc ttcagaacag cgtgacaatg ccattctgtt tgaatcccag acgccgcaga
     4081 atctgaatgt gccggcactg attacccagg ataccgacgg cggcagtgga gacggcgccg
     4141 ctgtctctcc gcagcaggcg tcacagaatg tgacgtccta cctggtggga gaccgcagcg
     4201 gaagtgcatc cggtccggtc gcagtcattc cgtctgcatc cggcagcggt acagtgattt
     4261 ccggcggaac atccggaaac tccggtacag ccgggaaaag cgatcagaac ggaaagggca
     4321 acgcaggcgg aacttccgga aacggaaaca attccggcgg cagccagaat aacggcggcg
     4381 gtcagacgga cacgcctgga acagatacac agccggagca gcccgccgca ccgtcctatg
     4441 acaacaatta cacggaaaaa acgccggaac agcccagcga tatttatgaa aacctgttga
     4501 ttcc
//
LOCUS       DAWENO010000173         4499 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7075_length_4499_cov_12.905266, whole genome shotgun sequence.
ACCESSION   DAWENO010000173 DAWENO010000000
VERSION     DAWENO010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7075_length_4499_cov_12.905266"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1014 a         1258 c         1282 g          945 t
ORIGIN      
        1 ggacggagga gtcaacccgg gcgaacactg ttcgccccta cagagtcgtt tcacccattg
       61 ccaaacgcaa cccatacgca aaacaaaaac tgcaaaacca tccttgacga atcgtcggga
      121 atatggtacg ataaagacgg taaaaaacgt caagagggaa catcgctgtg acgttccctc
      181 tttttatttg taattcggag tgcattcccg taccccggca cggctgccgg agggtgcggc
      241 gcagactctg catcagagaa agggcagcaa tatgatttat ttggtagaag acgatgatag
      301 catccgggag ctggtgatct atacgctgcg cagcaccggc atggaggcaa aggggttcgg
      361 gctgccgtcg gcgttctggc gtgcgatggc agaggagaca ccggaactgg tactgctgga
      421 cattatgctg ccggaagagg atggtctgga gatcctcaag aagctccgca gccgccaggc
      481 gaccaagacg ctgccggtca ttatgctcac cgccaaggga acagagtatg accaggtggt
      541 ggggctggac aatggcgcag atgactatat ccccaagccg ttcggcatga tggcgctggt
      601 ggcacgcatc aaggcggtgc tgcgccgtac ggaaaagccg gtggaagaaa aggtgctgcg
      661 gctgggcgaa ctgagcgtca atcccacacg gcacgtggta caggtgagcg gcgagaccgt
      721 gaaccttacc ctcaaggagt tcgaactgct gtgtctgctg ctggacaatc ccggcgtggt
      781 ctttacaaga gaccagctgt tgaaccagat ctggggctat tcctttgacg gcgagagccg
      841 cacggtggat gtccatgtgc gccacctgcg tcagaaactg ggggactgcg gcaactatat
      901 cgagaccgtc cggggcatcg gctatcgttc gggggatggt aaggcatgac cagaaagatc
      961 tttcagtcca tgatcgccgt ggtggtgtct gtgctgctgc tgagcctggc actcatcacc
     1021 ggcgtactct acgaacactt tgaatctgcc atgctggatc agatgcgcac cacggcgtcc
     1081 tttgtggagc agggtgtgaa agaggacggc atgaagtatc tggacgagat gtcgtccagc
     1141 cagtgccgca tcacctggat cgaggcagac ggcacggtaa aattcgacaa ccgcagcgac
     1201 cccggcacta tggaaaacca cgccgaccgg gcagaggtga aggaggcgct ggaaaacgag
     1261 tccggcacca gtatccgccg ctcgtccacc ctgtcggagc ataccctcta ttacgccaag
     1321 cggatgcagg acggcacggt gctgcgtctc gccatgtctc agcgctcggt gctgttcctc
     1381 atgggcggca tgatctcgcc ggtggtgttt atcttcctgg cggcgtgcgc ccttgccggg
     1441 gttttgtcct accgggtgtc caagaagatc gtgaaaccgc tgggcagcat cgacttgcag
     1501 catccggaac aggtggagac ctatgacgag ctttcgccgt ttttacagcg catcgctgca
     1561 cagaaccgag aaattcgcag ccagatggaa gagatccgca agcagcagca ggaattttcc
     1621 atgattacgg agaatatgag cgagggactg tttgtggtag accggaacta tcagattttg
     1681 tcctataacc ggagcgctgt gcgcattttt ggcatggcgc cggaaagcgt ccccgaaaat
     1741 ctgctggcgg tgaaccggag cgagggattc cgcagcgtgg tggacagcgc cctcaagggt
     1801 cgccatgccc aggaaaacct ggaactggac ggcagagtct atcagatcat tgccagcccg
     1861 gtgtgccagc aggaggatgc gctggatctg gtgggtgcgg tgatcctgac cctggatgtg
     1921 acggaaaaag aggcgcagga acagtatcgc cgggagttta cggcgaatgt ttcccacgag
     1981 ctgaaaacgc cgctgacttc catttccggc attgcggaga tcatccggaa cggcatcgtc
     2041 agaccggagg acatccctca ttttgccggc aagatctatg acgagtccca gcggctcatt
     2101 accctaatcg gggatatcat caagctgtcc cggctggacg aaaaccaggt ccccatggaa
     2161 cgggaatcgg tggacttgct ggagactgca cgggatgtgg tgcagcagct ggcatccgtt
     2221 gcccggaaaa acggggtcac gctggtgacc aacggcagcc acggcgtggt gaacggcgtg
     2281 cgccaggtgc tgggggagat ggtgtacaat ctctgcgaaa atgcggtgaa gtacaaccgc
     2341 cccggcggca gggtctgggt ggatgtccgc caggcggcag accatgtgga actgtgcgtc
     2401 aaggataccg gcatcggcat cccggcggca gagcagggac gcatcttcga gcggttctac
     2461 cgggtggaca agagccactc caaggcggtg ggcggcacag gtctggggct gtccatcgtc
     2521 aagcacggcg cagcgctcca ccatgcgacc atccacgtgg acagcgaacc cggcgagggg
     2581 actgccatca cgctgacgtt cccggcaggg taagaaaaac ggtgcgaaaa atgaaaaaag
     2641 ccccttgaca tgaaccaatc ccaaatcgtt agattttttt gactaacttt taggaggcgg
     2701 ttcaacaaaa ggggtttttg tttgacattt tttgcaaagt gtggtatact gggggtctga
     2761 gggcatacct gaaacggtag gcggtttgaa aattcttttg cttctgctcc cactgctgaa
     2821 tttacaacag ttgggaggtg attgctatga tggatcagta cgtcactttt gacgacttgc
     2881 tgaaaatttg cagtattctg ctaaatctcg caagtctgat cttttacatt tactataatc
     2941 ataaaaatag taatgcagat caccgcaatg acgagaataa aaagaaataa ccgcccctaa
     3001 gcgaccaaac ttgcggcggt taaatctttt ttaactcaaa gcagtgggag cagaccgtct
     3061 actggtatgc tctctttctc tttttattat acactgtttt caccgaattg tcaagtcttt
     3121 tttgaaaagc gccgttcaca cagtgccaaa caccgccgca cgcagttttc gcaccacgtc
     3181 gctgccgctg ccgccgcagg tccagcagca ccgtgccgtc ctggggcgac acgcagaaat
     3241 acggtcccgt ccagccgtcc cagccgaatt ccccggcagg cacattggtc atgcatgcca
     3301 gcggatcctc cagcacccgg agcaaattcc cgtagctgta gcccttcagc gaatcccagt
     3361 ccacccactt tttctgcgcc tcgttcagct ggggcgtggt gagatagcgc caggtgtttt
     3421 caccgatgag ccgcacgccg cttgcacgat gcagcccttt tccagccagc attgcggcaa
     3481 actgcaaata gtccggcagc gtggagacca gcccggcacc ggcagagaaa aacgccggct
     3541 ttttccggta gtccgtcatg cccagatgcg gatccggctc gaccgccaga ccgccccggc
     3601 tgccgtccca tacatattga tagagctgcg ccagccggga gcacttttcc tccggcacgt
     3661 agaaatccgt atccgtcatt cccaggggct ggaacacccg tttccggtac agcgcatcca
     3721 aaggcatatc cgccgccttt tccagcactg cgcccagaac atctgctgat gcgccgtaga
     3781 accacttttc tccgggctgg aacatcagtg gcacatcctc gcccacacgc cgtgcgaatt
     3841 cctgggtgtc caccgggtgc gccgtatcgt tccgccggtt gatctcgtca taaaatgcgc
     3901 ccatcttctt ttgcgagacg ctccactggt cgggatacgg aatgcccgaa gtcatgttca
     3961 gcagatcctg gatgcggatt tcccggtttg ccgggacgag tttgccgtcc gcatccacca
     4021 ttttttcccg gaaagacgga atgtaccagg agaccgggtc gctgagttcc agcagtccgt
     4081 cttccaacag ctgcatggtc accaccgccg tcaccggctt tgtcatggaa aacatccgga
     4141 agatgctgtc tgtccgcatg ggaatctgcc gctctttgtc cgccatgcca tacgcccgga
     4201 acagcagctg ctcctggtca tgcagcaccg ccacacacgc tccggcaatt ttatcctccc
     4261 ggataccctt ttccacaata cgttctgccc gctgttccaa caggctctga gactgctttt
     4321 ccatgtgcag cgccgccttt ctttccaaaa ctccgcataa caaaaaatcc gtatcacaaa
     4381 catgatacgg attttttggt gagatatgag ggatttgaac cctcgaccct acgcttaaaa
     4441 ggcgtatgct ctgccaactg agctacatcc gccacatgca gctgtggtct ttatatcag
//
LOCUS       DAWENO010000174         4466 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7128_length_4466_cov_7.090002, whole genome shotgun sequence.
ACCESSION   DAWENO010000174 DAWENO010000000
VERSION     DAWENO010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7128_length_4466_cov_7.090002"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1169 a         1167 c         1057 g         1073 t
ORIGIN      
        1 gtgcattcct gcaagtgtga aaaatcctgc cttttgacca ttgtcaaaaa ctgtgtcatc
       61 tgatccagac tcagacgcca ttcttcggga ctgagcagtg cggcattcag cttttcgctg
      121 ctcacagaaa acgaaaaatc atctgttttc cgggacattt ccacctgcac cagttggaga
      181 acatcttttt ccagtgaata ctgaaacgaa tcctcgcctt tggtcagcgt cagcaatggc
      241 ttttcccact tgctgctgtc ccatatcatg gcatctgcgg cgcagaaatc ccggtcatac
      301 aacacgacct gttccgtgtc cgccaatgtt gccaggtaat aggacgcagt cagcgcctgg
      361 atgtcctggt agacaaaggg tgctgccaca ttgccatcca ggtcgagaac gccaaaacga
      421 tcgccgtcct cggtttcttg ttttgcagcc agataatccg ttcctacaaa ttggagttgt
      481 ttccactccg gttcgagaat cgtatttcct gccgcatccg acacgccgta aaggtggttg
      541 tccccctgta cgatttcatt gccgtttgca ttgattgccg tatccgatgc actgttctgg
      601 ttactcccag atcttttcga aacattcgcc gaaaagctaa tggtcgcagc aatcaaaatc
      661 accagcacca gcaccagaat gcctgctgca agccgggtgt gtttattcat acacagcgct
      721 cctgcttatg attccatgtc cttgcgggca tctttttcgg tttcctgcac cagataaatc
      781 gggcgtttct tggtctccag ataggtcttt gctaggtact gtcccgcaat accaacgcag
      841 aacagctgga gaccacccat aaagaagatc gcacagatca gcgttgcaaa accctgcacc
      901 ggatcaccga atgcgagttt cttaatgatg atgatgataa tcatgataaa tgcaaccaga
      961 cagcttgcca cgccaagaat tgacgaaatg gtcagcggtg ctgtggagaa agcgaagaat
     1021 ccatccagcg catagcggaa caatttccag aatgaccagg tgctggttcc tgctgcacgc
     1081 tccacattct catattcgat atatttagta cggaatccca cccagctgaa aatacctttg
     1141 gaaaaccggt tgtattcttt catcgacaga atcgcatcta ccatctgccg tgtcataaag
     1201 cggaaatcct gtgcgccgtc cacgatctcg gtctgcgaaa tgcgtttcat gatcttgtag
     1261 aatttccggg agaaccagga gagcagcttc ggctcgccct ttcggctgac acgccgtgtg
     1321 gtagcgcagt catattcgcc gcttaacacg atgttgtaca tcttcggcag aaatgccggc
     1381 ggatgctgta ggtcagcgtc catgatgaca acataatcac cagtggcatt ttccagaccg
     1441 gcgtacatac cggattcctt tccgaagttc cgggagaagg aaatataccg gacacgctca
     1501 tctccacgtg ccagttcccg cagtttggcg agggttccgt cacgagagcc atcgttgatg
     1561 aacagcagtt caaatccggt atccggatgt tcttcctgga tctgctgcat cactttctga
     1621 atttctatgt aaaaaagcgg caaaacttct tgttcgttat aacatggtac aacaatggaa
     1681 atctttttca taacgttcct ttccgttcca cagccagccc cttgtgaatc cggctgttat
     1741 attcagcata tccaaaaaag caaaacttcc atgacacgct attatttatt ataccacctt
     1801 ttctgacatt ttacaatacc ctaacaggga tatttttttc gttttccaaa aaaatatgtg
     1861 aacaaataag gctgtctttt gaattgcagc gtcctctatg ccttacgctt ttgcaggaat
     1921 gcctccagac ggcgcttata gttggaagaa cggtgcgtca tccggtcaac cagttccagc
     1981 agctgccggt ctgtcaccag atccagtgcc tgaacctccg attcctgcat ggtgcagtcc
     2041 gacaaagcaa agtccgtctt gatatagtac acatcatggt agcagctgcg ccggcggtcc
     2101 cggaacaaaa agcgcatttt ctctctgggc agcgtcagac cgatttcttc ctttgcctca
     2161 cgcagtgcac cgtccagact cgtttccccg gcatccactg cgccggcagt aatgtcccac
     2221 tgtcccggac gggtcgcctt ggtatccgac cgtttctgca ccagaaacag tccgtcctgg
     2281 ttctccagga tcacgtgggc gcagagaaag tactcccctt tggacagaaa tgtgctgcgt
     2341 tctgctgttc ttccggtaag gtttccgtgt tcgtcataga tatccagaat ttccatatta
     2401 taccgtattc ctttcaacaa tgatacgacc attctattcc tttatattgt atcacgaaat
     2461 tctttccctg gtcaagatac atttctgaat tttctttgaa aaatcccgtg acaccgacag
     2521 gctgtgccta cctcttttct gaaaattaca tgaaaatttg gaaattgcgc ttttcaatcc
     2581 aaacaaaatg tggtataatg aaatatcaca acattcccgg catatgccgg gacacatatg
     2641 gagggaactg caatggcaaa ctggagagaa cggcttacac aatttatgca gggcagatac
     2701 ggcggaaatg atacattcaa caaggcgttg ttcggcgtgt ttctgttttt cctgctgctg
     2761 gcgattttca caggaagaac cgcagtcagc accacgctga atctgatcgc aattgccgta
     2821 ctgatctatt cctacttccg gatcttctcc cggaatattg cacgccgcca ggctgaaaac
     2881 cagaagttcc tggagatcac tgctccggtt cgctccctgt tcggcaagaa aagcgccgac
     2941 aagacacacc gcatcttcag ctgtccgaaa tgcggtcaga aaatgcgtgt gccgatgggc
     3001 aaggggaaaa tcgagatcac ctgtccgaac tgccgcaata agttcatcaa gcgaagctga
     3061 ggaaaacgat gtacgggtat attcgggcat acaaaccgga aatgcggttt cgggaatacg
     3121 acatctacca cggctgttac tgcggtctgt gcgatgcgct ccacaagcgg cacggcgcag
     3181 ctgcacgctg gacgctgagc tatgacatga catttctcat cctgctgctg acagggctgt
     3241 atgaaccggc agaggaaaaa gcagagcggc gctgcatagc gcaccctttt cataaaagtc
     3301 tgcacattca gagtgaaatc acagcgtatg ccgcagatat gagtattctg ttgttccggg
     3361 acaaatgtat ggacgactgg aacgacgagc gaaaaatcac aaggcgtgcc gccgctgaga
     3421 tgttccggaa aagctaccgc agaatccagg cgcagtatcc gcagaaatgc aaagcggtac
     3481 aggcacagct ggatctgctg catcagatgg aacagcaaaa cgaatcgaat ccggatctgc
     3541 cggcaggctg tttcggcacg ctgctgggcg agcttttcgt ctggaaagag gacgaatggg
     3601 cagacaatct gcgccaggtc gggttctatc tgggcaaatt catctacctg ctggacgcat
     3661 atgacgacct ggaacacgac agaaaaaaga actgctacaa tcccctgcgc agcatggcgg
     3721 aacacgatcc gcagttttca cagacctgca aatccctttt gaaaatgatg atggcatcct
     3781 gcgcagaatc atttgaacgg ctgccgatcc tgcactatga cgagatctta cggaacattt
     3841 tatatgcagg cgtctggccg aaattctatc aacgcaaaaa agaactggaa gagactgaaa
     3901 aacaggagaa acaaaaacat ggatccatat gaagtactgg gaatcccccg aaacgcatcc
     3961 gaccaggaag taaaaaaggc ataccgtgcg ctgagccgga agtatcatcc ggacgccaat
     4021 gtcgataagc cgaatccgga aatgtacgag gaaaaattca agcagatcca gcaggcgtat
     4081 cagaccatca tggacgagcg ccagcacaaa tccgacagcg aatacggcgg attctacggg
     4141 cagcgctctt ccgctggaaa cggcgatgag aacagcgtac atttgcaggc ggctgtgaat
     4201 ttcatccgga acggcagatt cacagaggct gtccgggtac tgaacggcat cagcaaccga
     4261 aatgcacagt ggtattatgt ttctgccgtt gcaaactccg gtgccggaaa caatgcaact
     4321 gcactccagt acgcaaggac tgcggcgcag atggagccga acaactatga atatcagaaa
     4381 ctggtgcagc agctggaatc ccccggtttc tcttatcagc agatgcagca gccctatatg
     4441 ggcggcagtc ttgatgacag cggatg
//
LOCUS       DAWENO010000175         4441 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7173_length_4441_cov_10.067715, whole genome shotgun sequence.
ACCESSION   DAWENO010000175 DAWENO010000000
VERSION     DAWENO010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7173_length_4441_cov_10.067715"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3416..3425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1064 a         1188 c         1182 g          997 t
ORIGIN      
        1 agaactgggg tgcacgacgt gcagccttca gaccgtactt cttacgttcc ttcattctcg
       61 ggtcacgagt gaggaatcca gccttcttca ggaccggacg cagttccggg ctgtaaacca
      121 gcagcgcacg ggagatgccg tgacggatcg caccagcctg accggtaaca ccgccgcctg
      181 caacagtgca aacaacatcc atcttgtcca tattttctgt cagattcagc ggctgacgaa
      241 cgatcagctt cagagtatcc agaccgaagt attcatcgat gcttctgccg ttgatggtga
      301 tgctgccgtt gcccggatac agacgaactc ttgctacaga gtgcttccgt ctgccggtgc
      361 cataatagta tttaacctta gattcgtaca tcttttctgc tcctttccct tagaactcgc
      421 aaacttccgg cttctgagcc gcctgcttgt gttctgcgcc cttataagta cgcagacgct
      481 tcagctggtt tgcgccctgg gtattgtggg ggagcattcc cttgacagcg atcatcattg
      541 cttcatctgc tttttcttcc atcataacct tatactgtgt cttcttcaga ccaccgatcc
      601 aaccggtgtg ccagtaatag tacttcttct ccagcttgtt gccggtcaga actgccttgt
      661 cacagttgat gatgattacg ttatcgccgc agtcaacgtg cggtgcgaat gtgggcttgt
      721 gcttaccacg cagcaggtgt gcagcctttg cagccacacg gcccagcggc ttgccggcag
      781 cgtccagcac ataccacttg cgatctactt cgccagcctt cggcatagtt gttgccatag
      841 tcaattcctc cattgaaact tctatcaaaa ataatctcga tcccggaaaa acgggatcag
      901 gcagagggca gttgggttag actgcactgc cggcatttcg caagattata accggtctct
      961 ctgacagtac aagaactatt ataccacaga atcgggcgaa atgtcaagcc gaaatccgca
     1021 gttttttttc gattttcaga ggaaatcggc tgtgaatttt gtgcatattg ccattttgcc
     1081 cggcaacccc ttttgccggc ttgcaatttc cacagaactg cgatataata gtataggcaa
     1141 ttccgcacaa aacccgtcgg ttggctttgt acggttttgc cggtatcgac aggactctgc
     1201 ggtcacgcag gaacgggagg caacaaacat gagacaagaa ccatcttcgg agaactgccg
     1261 gctggtctat ctgacacgcc gtgcctatga gaccggagag atccggctgc tgctccgcac
     1321 ggacaatgtc tgtgcggaaa aagacaccgg ctggcagctg ctccagggca gcgagaccgg
     1381 gctggagctg tccaatcccg gaaacagcct gctggtctct gtggcacgtg cgctggagat
     1441 cgagccgaag cttgcgccgt ttctgaccgc agatacgccg ccgcagaagg cggcatatgc
     1501 ctatgacgaa aaaaccgccg gctaccgtcc caccgcatac ccggaggacg tgccgacgga
     1561 gatatgacaa agcgggcgga gcgaatgctc agcctgtgat gacatggctt gcgtctgcgt
     1621 tggcgaggat catatagtac accagacagg caagcataca tataatgaaa aaagcgatca
     1681 caacacgcag ccggaaacgc agaaacggtt tcggcttgtg gatatcatca attcggtgca
     1741 gctgcttttg tcttttgcgc atgatacagg actcctttct gtggatgtcg gtattcctgt
     1801 tactattata aaggatggcg cagcattttg caagcattat ttttgcagca ccggaatttg
     1861 ccttgacaaa ggagaaaaac ttatgtgtgg ttttgtagga ttcaccaatc atattgacaa
     1921 ctcaaacgtg gtcctgggcg agatgctgga taagatcaag caccgtggcc cggacgctga
     1981 gggtacttat attgacgagg atattgcact gggacaccgc cgcctgagca tcatcgatgt
     2041 ttcggatgcc ggcacacagc cgctgtacag cgaggacggc aatctggcgc tgctgttcaa
     2101 cggcgagatc tataactacc aggagatccg ctcccgtctg atggaggcag gctatcactt
     2161 tgccacccag accgactctg aggtgctgat ctacggctac caggagtacg gcgcagaact
     2221 gctccagcag ctgcgtggaa tgttctcctt cgtgatctgg gataagcgtg agaaaaaact
     2281 cttcggcgca agagatttct ttggcatcaa gcccatgtac tatgcacaga tgggagagac
     2341 attcctgttc ggctcggaaa tcaagagctt tctgtgccat ccgcacttca aaaaggtgct
     2401 gaacaccgcc gcactggaaa actatctgac cttccagtat tcgccctgtg acgagacctt
     2461 tttccagggc gtgtataagc tgccgccggc acactatttc acctatcaga acggcaagtt
     2521 cgagatgaaa cgctactggg atgtgcattt caaggcagac cagaaacccg acctggatac
     2581 ctgggtcaac cgcatttccg ataccttcca cgacagcgta aaggcgcata agatcgccga
     2641 tgtagaagtt ggctctttcc tgtccagcgg cgtggattcc agctatgtgg cagctatcgc
     2701 cgatgtggat aagaccttta ctgtgggatt cggcacggaa gagcgctaca acgagatcgg
     2761 ctgggcaaag ggcttttctg aggcgatcgg caagaagaat tacaacaagg tcatttcccc
     2821 ggaggaatac tgggaaaagc tgcccatgat ccagtaccac atggatgaac cgctggcaga
     2881 cccggcagcc attgccctgt acttcgtgtg caatctggca tcccagtcgc tgaaagtcgt
     2941 gctgtccggc gagggcgcag acgagatctt cggcggctat aatgtctaca gcgaaccggg
     3001 cgggtctgcc tatgacaagc tgccccgtgg actgcgccgt ggcatcggtc acattgcaga
     3061 gaaaatgccg gcgcaccgtg gcagaaactt cctggtacgc aagggcaagg atctggaaga
     3121 gcgctttatc ggcaacgcct atatgttcac cccggaagag cgcaaggcac tgctgaagat
     3181 tcgcactaat gcgccggatc ccatggcgat caccaagccg ttctacgaca aggtgcagga
     3241 tcaggacgac gtgaccaaga tgcagtacct ggatctccat atgtggatgg cgggcgacat
     3301 cctgctgaaa gccgacaaga tgagcatggc gaattccctg gagctgcgtg tgccgttcct
     3361 ggataaaaaa gtcatggaac tggcagaaca gattcccaca cgctatcgtg tgacannnnn
     3421 nnnnntgtga cacgggaggc tgtcaccgac gaaaagaccc cgtatatcac caagtatgcc
     3481 atgcgtctgg ctgccaagaa ggatacaccg ccgcagactg ccaagaccgc agccaagaaa
     3541 aagctgggat tcccggtgcc gatccgtgta tggctaaagg aagacaagta ctacagcatc
     3601 gtccgggaac gctttgagag cgacgaggca aaccgctttt tccacacgga aaagctggtg
     3661 cgcctgctgg acgatcaccg tgccggaaag gcagacaaca gccggaagat ctggacgctg
     3721 tatatgttcc tggtatggta tcagatttac ttcccggagg cttgtggcgc ataagtgctg
     3781 cataaaggag ggatagacct atgccaagcc atgtaaccta tgcctatatc cggcagaatc
     3841 ccgatatccg ggagtatatc cgccgtgccg atatgtccct ggcggcgatc ggctatacgg
     3901 agcattcctt tgcccatgtg gaaaaggcag cgcacaatgc tgccatgatc ctggagacgc
     3961 tgcaataccc ggcacgccag gtggagctgg caaagatcgc cggatttctc cacgatatcg
     4021 gcaacgtcat caaccggaac gaccacgccc agagcggcgc agtcatggcg tttcgtctcc
     4081 tggaccgtct ggaaatgccg gtggatgaga tctgttccat catttccgcc atcggcaacc
     4141 acgatgaggg aacggcacag ccggtggatg ccatttcggc agcgctgatt atcgccgata
     4201 aaaccgatgt gcgccgcagc cgtgtgcgca ataccgattt catgacgttc gatatccatg
     4261 accgtgtcaa ctatgcggtg gaatattcca acctgcattt cagcgatgac cagaagtcca
     4321 tcgtactgga tctgaccatt gacaccgaga tttcttccgt gctggaatat ttcgagatct
     4381 tcatggagcg aatgctgctg tccaagcgtg cagcgctgtt ctttgacaag aaattcaaga
     4441 t
//
LOCUS       DAWENO010000176         4374 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7291_length_4374_cov_8.219727, whole genome shotgun sequence.
ACCESSION   DAWENO010000176 DAWENO010000000
VERSION     DAWENO010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7291_length_4374_cov_8.219727"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          891 a         1313 c         1075 g         1095 t
ORIGIN      
        1 aataccagtg ccgcagcaac tgcgctgctt tgccatttct tcatttatga ttcctcattt
       61 ccatcagtcc cctgcccgta aggtgcaaag ggcgcattgg ggtcgtatgt cttttcatgc
      121 cgcagataat acacattggg ccacttgatg ttcgggttcg gaatgcttcc ggcgatccgc
      181 tcattgggat cttccccgtc ccgcacacgc cgtgccacga tcaccaggcg tgccgtatca
      241 aaggcattat tgcaggtaga aagggtcagc agctggtcgc cgtaggtcac atccacattg
      301 gtgatgcgca gcgtccggcg ctttacctca ttgacatagt catagaattc cttctcaccc
      361 gaaaagtcaa tggcgttcca atattcgaac cgggtatctg tggtatcctc ggcgtctgca
      421 ataaagtagc cgaagatctt gtactgatag gtctcataat tggaattcag ctggatgata
      481 gggtgttcat cgtaataatt ttcatcggta cggtaatatt tcagcgtacc gaacatagac
      541 aggtcacgca tattgtgacc gtaaataatc aggttgtccg agctgggact cttcagcgtg
      601 ccgtcctcgc ccacatcgtc aaaggtacag cggtaatcca ggaagatcga acccggcttg
      661 gaatcctctt tctgcacatt ccggtagaga taatactcat tgcccggctc atcgtccaga
      721 tggtgcagca ccggataatc cactttggta tccggaatcg agatccagcc gttgatctct
      781 gaattcattg ccagcagatc cgccgcaccc ggcaggagcg tatagatctt ttcggtaggc
      841 tcttctgccg caggctgtgt ctcctgcact gcggtggtcg tctgtaccga ctggttggcg
      901 tggtaattgg agccgatgtc ctcatacagc ttgtctgcac ggaaaatgtc cacaaagtac
      961 aggacgatca gcaccatgca gaccagaaac accgtcgctg aaaaaagaaa gatacttttc
     1021 cgcaccactt cgcctccgga atcgccacgc tgtggaaaca gccccagcat tttctgacca
     1081 aacgtctgtt ttctcggttt cggcggcatg ggcggcggcg gatacttttc cgggtgttcc
     1141 ttctgatcct tgacctgttg gatctgctgc gccacacgct gtgtgaccgc ctcctcttcc
     1201 tcttcctgtg caatgtggaa caggctggtg cggatcgccc ggatcttgtc ctccggctca
     1261 gcaggaacct cctccggcgc tgtctctgag atctctgcgg tatcttctgc cgctgtttcc
     1321 gccgcatccg cagtctcaga tccttctgcc ggttcttccg caatgctgcg ctccggctgt
     1381 tccggcgttt ccgccgcctc agtcggtgtt acagcatttt tttcttcttc ctgcattctg
     1441 cttcctccat caggattcgt tctgcgatgc cgaatgcaca cacagccgta cccagacgga
     1501 tttcttcccg tgtgcgcagt ccgtaccgcc aaagctgcaa gtgcatgact gcctcttgct
     1561 ccccatacag aatcccagca tagaccgtcc ccacaggctg ttcgggcgta ccgccgcccg
     1621 gccctgccag tccagtcaca gagagacaga gatcagcgtc cgactgacgg cgcagtccct
     1681 gcaccatcgc cgcagccacc tctgcgctca ccacaccatg cgctgcgatc agctctgccg
     1741 gaacgcccag cagtttggtc ttcatccgct ccgcataagt acacagtccc agttcaaata
     1801 catgggatgc acccggcacg gaggtaatca tctgcgacag cataccgccc gtacagctct
     1861 ctgccgtggc gatggtctga tgttctccat ctaataattg tacaatatgt gcaagggttt
     1921 tgtcaagtgc tttttctgtg tcttgataaa tacgacacac ttttttttca tctattaagg
     1981 tatatgccat atttcacagc accttcctgt ctgctcagtt cagttcgaaa accttcatgg
     2041 ttgtgccggc gactgccttg tccaccattt ccgtgaagtc gaactttgcc tgcgcctttt
     2101 ccacttcttc tagagacggg cgcaccttcg ggtgtttcgg cgtaaacact gtgcagcagt
     2161 cctcatacgg cagtgtggaa gtttcaaagg tgccgatggc acgggcagtt tccacgatct
     2221 ccgtcttatc cgaaccgatc agcggacgga ataccgggat cttgctgacg gcatcggtgc
     2281 aggcgatcgc ctgcatggtc tggcttgcca cctgtcccac gctctcgccg gtaatcagcg
     2341 ccaggcagtt gtcctttaca gcgatcttct gtgcaatttc catcatcagc cgccgcataa
     2401 taacggtgaa atactcttcc ggacaatact tcttgattgc ctcctggatc tctgtaaacg
     2461 gtacgcagaa aaatgcaatg ttgccgcagt acggcgtcag ttcctggcag agctgttcca
     2521 ccttttcctg cgcacggtcg gaggtataag gcgggctgac atagtgaatg gcggaaatat
     2581 gtacgccacg gcgtgccatg cgatagcctg ccaccgggct gtcaatgccg ccggacagga
     2641 gcagaagtgc cttgccgctg ctgccgaccg gcagaccgcc tgcacccttg aggtttgccc
     2701 cgtgaacaaa tgcgtgggta tcacggattt ctaccattac agtcacgtcc ggatggtgta
     2761 catcaacgga aaggtgcgga tatgcgtcca gcaggatacc gcccaactct gcgcagatct
     2821 ccggcgactt cagcggaaac gccttgtccg cacgctttgc ctctacctta aaggtatttg
     2881 caccctgcaa aacctcttcc agataagaga ccgccttggc agaaatgtcc tccatggtct
     2941 tttcgcagac gcaagcacgg cacagcgccg caatgccaaa gacgcactgg agctgttcca
     3001 gaacttcgtc caggtcgatg gtatctgaca gcggttccat ataaatggtg gactgcgcac
     3061 gattcagttt gaatgcgccg aactttttca gacggcggcg caggttcttc atcagaatat
     3121 cttcgaatgt gccacggttc aaccctttca gtgccatctc gccatatttt gccaaaatca
     3181 attcttttgc catgtatttt ctccttctat tcccggcagt tctgccgtat cagtgcatca
     3241 gatctttctg tgcatccttc aatccctgta ccagcgccgc aatatcctct tctgtattgt
     3301 cggaacaaaa gctgatgcgc agggcgctgt ccacttctgc cggctttgcg ccgaacagtt
     3361 ccagaacacc gctgctggcg cccttggaac aggcagagcc gctggaaata tagacctcac
     3421 gctgttccag atagtgcagc acgatctccg aacgatatcc cagcagggaa atattcagca
     3481 catacggaga cgccgacggc aggtcacgaa tgataacgcc gtccatctcc ttcagctgtt
     3541 cgatcaggct gctgcgcagt ccggaaacgg catcgtaccg ctcatccatg gtcagcacca
     3601 gcttttccgc tgctgcgcca aatgctgcaa tcaccggaac gccctccgta cccggacgca
     3661 gtcccttttc ctgccggctg ccgtacagca ccggtttcag ccgcacgccc tggcgaatat
     3721 acagcgctcc ggcacctttc aatgcgtgta ccttatgacc gctaagggat accaggtctg
     3781 cgcagagggt tctcggctta aatgatacct ttaagaaacc ctgtactgca tcgcagtggg
     3841 tgatgatgtc cgagtcacgc cgtttcacgg tgcggaacac ttcttccacc ggcatccggt
     3901 aaccgttttc attgttcacc agcatgatgc tcaccatgca ggtgtccttg tccacttctt
     3961 tcagaaagtc ctctgcatgg tacagtccat cctctgccgg cggcacggaa actaccgtga
     4021 agcccagcgt ttccagatgc gccatagtct ccctgaccga tgcgtgttcc acggcgctgg
     4081 tgatgatctt cttccgcttt ctgccgtatg cggcagctgc gccgcagagc gccaggttgg
     4141 tactctccgt tgcgccggat gtaaacacga tctcctgcgc cttacatccc agcgctccgg
     4201 caacctggtt gcgtgcatat tctacttcat gctgtgcctc cagccccttg cggtgcagag
     4261 aggacgggtt gccgtagtgt gatgagatac agctcatcac agcagcagcc gcctctgcgc
     4321 agggtttggt cgtcgcagca ttgtccagat aatgttccat atttttcccc ctat
//
LOCUS       DAWENO010000177         4348 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7321_length_4348_cov_10.363848, whole genome shotgun sequence.
ACCESSION   DAWENO010000177 DAWENO010000000
VERSION     DAWENO010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7321_length_4348_cov_10.363848"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3944..3953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1011 a         1170 c         1157 g         1000 t
ORIGIN      
        1 cgtaggtgac aaaagggacc gctcagtacg agatgcccga cagggcagag gagttatctt
       61 gtccccccct tgaaaaggcg cccgaagggt gtgccttgtg ctgggatgtc ccgaagggac
      121 agggggatac aattgtaggg gcgaacaatg ttcacccgaa actcctcagt caagccttac
      181 ggcttgccag ctcctctaaa agaggagcca agaaaaaatc tgaaaagctg gcacgcagca
      241 ccggtcaaag tcgcctgttt ccggcaaaat atctgatgat cctgggcggt tttacgggga
      301 tcttgttcct gttcaccgtg atccctgccc ggttttcggc gcagccggat cacgccgcag
      361 aatattccgc cgccaccccc gtcacgaccc tttccgccga agagagcgcc gccgcaccgg
      421 aaagcgaacc ggacaaggcg gcgctgtgcg ggacgtaccg gatgctggac atcaccagcg
      481 gcagggtgga agagatctcc gtccgggact atgtgatcgg cgcagtttgt gcagagatgc
      541 cggcgacctt tgagccggat gcgctgaagg cacaggcagt ggcggcgcac acctacgccg
      601 agcgacagca tctcctggaa agcgccagcc ccaccgcagc gctttgcggc gcagattttt
      661 ccaacgacag cagccagtac caagcatatt tcacggaaaa ccaggcacgg cagtactacg
      721 gggacaactt tgagaccgcc tatgccaagg tcacccaggc ggtggacgat gtactgccct
      781 atgtgctgga atacgacggc gcacccatta ttgcggcgtt ctgctccatg tccgccggcg
      841 tcacagaaag tgcagaaaac atctggggca gcaaggtgga atatctggtt ccggtggaga
      901 gccaggcgga cacctctgcc ccgcgctatc tggaaacggc gcagttcacc ggagacgagt
      961 tccaggcggc ggtgcagtcc atgctgcccg gtgcagtctg ggaagaggac atggcgaaat
     1021 gggtgcagat cggagcaagt tccccgtccg ggactgtgct ggaagtgacg gtgggcggcg
     1081 tttcctgcac cgggcagcag ctgcgtgagg cgctggggct gcggtcggcg gtattttccg
     1141 tggaggtgct ggacggcagg ctggttttca ccaccaaagg ctacggtcac ggcgtaggca
     1201 tgagtcagtt cggcgcaaac gccatggcaa aggacggcgc aggctggcag gagatcctgg
     1261 cgcattatta tccggcggcg gaactgacag agggacaggt cgaatcgtga ttctgtctct
     1321 gttgcacaag tactttccac aattctttca acattttaac agatgaaaac aaacagtttt
     1381 gtggaaagtt ccgcagtgtt cgggaaaagg cgtgcatatt atacaatttt tgcataaaaa
     1441 ttatccggtc tgccccttga caaaccggat ggattcgggt ataataaaga caaagagagc
     1501 gtaccgaaaa cggtctgctc ccacatgaga atatggaaat agccgtctta gtttggtcgc
     1561 tcgagacggc tatttccgtt tattgaagct cagatacgca agatatgctc ggaacacttc
     1621 acaaacaatg ttaatgcact tgacaagaat gtccagattc attcgcattc actcccttcc
     1681 gggagttgga ttgaccgccg tagacctttc ggtacgctca tgctgttaat tatagcaaaa
     1741 ccggaccgtt ttgtcaagat ttcgacagaa caaattatcc ggtctgcccc ttgacaaacc
     1801 ggacggattc gggtataata aagacaaaga gagcgtaccg aaaacggtct gctcccaaat
     1861 ttagcactta aatagctgcc aaaagtcggg gaaggactgg gcggctattt tccttttcta
     1921 tcgtcatgct gcatgatctg aatgatcttg cagataaaat tcaaaagttc caaaacttcc
     1981 aataagctca tgacatcacc cccttcctgt aaaaaagcag gcgtgggagc aggattagac
     2041 cgccgttaca tacaactgta tgtttttcgg tacactcatg ctgtttatta tagcaaaacc
     2101 agaccgtttt gtcaagattt cggcaaaaca aattatccgg tctgcccctt gacaaaccgg
     2161 atggattcgg gtataataag gataaagaga gcgtaccgaa tggcggtcac tcccgataat
     2221 ttgactattt ttgaaatagc cgccttatgt ggaagttagg cggctatttc tcttttgtat
     2281 tgcgagtacg attcttactg ctgttttgca gaatcagaat cacattacaa atgacgttgc
     2341 ataaagtcaa aaactccatc catgtcataa cgcaacacct cccttgttac tgtgttttca
     2401 cacagccggg agttggattg accgccgata gacctttcgg tacgctcgtg atacctatta
     2461 tagcaaaatc agaccgcttt gtcaagaatt tgacagcttg agcgagtgtt ctgattaagc
     2521 gattccgggt atcacaaaac caaagagagt gcgccgaatg gcggtcactc ccgttaagtt
     2581 tagactgtta taagaaacag ccgccttatg taggagttag gcagctgttt ctcttttctg
     2641 ctgtagaata tctgcaaaca aaaagcggac gttttcccgt gtggggagac gtccgctttt
     2701 cgcagaatgt gtgacatttc atgaggatcg tcacaaaatg tctttataaa agttgtttcg
     2761 aaaatgtcat tacacttgcg caagcgtctg ccgcagaact gccagatcca gcccgtttac
     2821 cgtgccgtcc ccgttgcagt ctgccaacgc cgcagcgtcc ttgggcaggc tttccacgcc
     2881 cagaagatac ttctggagaa gcaccaggtc tgcggcggaa acgctgccgt ctccgttgca
     2941 gtcccccaaa agcggcttgt cagaatcacc ctttaccgcc aggagctgct gcatcagcgt
     3001 gtcatcatac atcttgcagt tgttccggtc gtagtgcagg tctgtgatat cccacagcac
     3061 cggacacata tcccgctcat atgctgcgcc gcagacagag gacaggaact tccggacgga
     3121 ctcctcttcc ttgttctttt tcgggcagcc gtattctccg ataatgaccg ggattccctt
     3181 gtccacatag gtggttttca gaagatccat gttccggttc agttcctcga agtctgcatc
     3241 tgttccccag gtggaacgca tcttgcccca gtctgcatcc tcttccagga tggcaaagcc
     3301 ggagggcgta tagtaatgca ccgagacagc gcagcggtgc gccgggtcgt ccggcatctg
     3361 gaacagactg tcacaggtca gttccacgtc ggtattatag ccggagatga gcaggtggcg
     3421 ctggggattg ttgccgccgg aggagcggat gatgtccaca aaggtctggt tgatctcatt
     3481 gaccagggca taggattccg ccttgccctc tgttccgccc cagcggttcc acaggctctc
     3541 ccagcccagt tcttcgttct gggactcgaa tatcagatag tcgtcataat cggcaaaggc
     3601 atcgctgacc tgtgtccaga tccgcttgta cttctccata cagttttcct tgtccgtggg
     3661 gaagttcgcc agccagccgc cgtcatagtg cagattgagg atgacataca gaccgcagtc
     3721 tctcgcccag tccaccacct cttgcaccgc agaaagatag ctgtcgctga tggtgtagtt
     3781 atcccccatc agattggtcc acgccaccgg aacacgaaga gtgtcgaatc cggcgtcagc
     3841 gtagccctgg atcatctgct gggtaatcac cggactgccc caggcggtct cataggattc
     3901 cggcgtgccg tcgccccact gggcgatcca gtcgccgcag gatnnnnnnn nnngttgatg
     3961 ccgatgccca tatcctttac cagctcggca gtcgtaatgt cccgcatcgg ggactgtttc
     4021 tggggcagcg ccgcagcaga aacggcacag ttgcaccaga gcataccggc agccactgcg
     4081 ccggcaagta tttttttcag attcatggtc cttttccctg cggcaaagcg ccgctgtgtc
     4141 actctatttt tatgccggca cggacacacg tccctgcttg cggaagtccg taggcgtact
     4201 gccgcagttc ttcttgaact ggcgcaggaa gctgtaggta cagccatagc cgcactgctc
     4261 tgcgatctca cccaccgtca gtccagtgga agaaagcagc tctttggcac gctgcatctt
     4321 cgctcaggtg acatccgtca tcaccgtg
//
LOCUS       DAWENO010000178         4284 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7427_length_4284_cov_10.173563, whole genome shotgun sequence.
ACCESSION   DAWENO010000178 DAWENO010000000
VERSION     DAWENO010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7427_length_4284_cov_10.173563"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    233..242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2850..2859
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          956 a         1165 c         1286 g          857 t
ORIGIN      
        1 ggggataacc cctcagtcaa gcctacggct tgccagctcc cctgttaggg gagcctatga
       61 acaagacaag ttctacaggt actttaatat cgaaaatcca cctctataaa aattgatttt
      121 ctttccccga cttgacaaaa ccgccgaaaa tgggtataat aaaaagtaat caactttttt
      181 atgatgctat tatgcagaat gttcacaaag tgttgcgttc tgcacagtcg ggnnnnnnnn
      241 nnctgcaaaa gagcggcttt gtgacccgga gggcggatgg gcagagccgc cggagatttc
      301 cggtgcagtt ttgccggcac gagaaacgca gcagagacag ccgagacgga attgccccgg
      361 gctgtcccaa ccgagagagg actgataaga tgaaaaaaga aacactgtta cactccaaga
      421 agatccagaa aatgattaca caatataagc acgatatgca gctggtggat cagctgactg
      481 ccgatgcagc gccccactat gagatcgagc aggaactcta tgtgaaacat gaggtaaagc
      541 gtgggctccg tgacctggac ggcaagggcg ttcgtgccgg gctgacgact attagcaagg
      601 tatctgcgac ccagatggtg gacggcaggg aagtgcctgc gccgggacag ctgctgtacc
      661 gtggcatccc catcgaagag ctggtgcagg gctttatcac ccatgaccgc tttgggttcg
      721 aagagatcgt ctatctgctg ctcatgggac atcttccgca ggaggaacag ctccgccagt
      781 tcaaggggct gctggcagac tatcaggaac tgcccaacca cttcaacagc ggcgttatta
      841 tgaagatgcc cagcgacaat atcatgaaca ccatggcgaa gtgcgtcctg gcgctccacg
      901 cctatgaccc caagccggat gacaccagtc tgcccaatgt gatgcggcag tgcatccagc
      961 tggtggcaca gttcccgtct ctggcggtgt tcggctatca ggcatccatg tacttccgga
     1021 acgacaagag tatgctcatc cggaatcccg acccgaacct ttccattgcg gagaatattc
     1081 tgtatatgat gcgcccggac ggcaaataca cccgtctgga ggcaaaactc ctggatctct
     1141 gcctggtgct gcacgcagaa cacggcggcg gcaacaactc tgcctttacg gtacacgtgg
     1201 tctcctcctc cggaacggat acctattcta ccattgcggc ggcgctgggt tcgctgaagg
     1261 gaccgaaaca cggcggcgca aacatcaagg tcatggaaat gttcgaggat ctgaaaaagc
     1321 actgtcccga ctggacggac gaggacgcac tccgggcata tctccaggga ctgctccggg
     1381 gcgagggctt cgaccacagc ggtctgatct acggcatggg tcatgcggtc tactccgtct
     1441 ccgaccccag agccgtaatt ctgaaatcct ttgtggaaca gctgtctaat gaaaagggct
     1501 gccacgacga gtaccagctc tatgcgtctg tggagcgtct cgccaaggaa gttatcaccc
     1561 agaaccggaa gatctataaa ggtgtctgcg ccaacgtgga cttctacagc ggctttgttt
     1621 atcggatgct ggggctgccc atggatctgt ttacgcccat cttcgccatt gcacgcatcg
     1681 ccggctggtc ggcgcaccgg ctggaagaac tcatcaactg ccggaagatc attcgcccgg
     1741 catatctttc cgtggcagag ccgcaggaat atgtgccgct ggaagagcga taatagaatc
     1801 gaggggaaac catggagaga ccatatatca tttgccacat gatgacgtcc gtagacggca
     1861 ggattgactg cgccatgacg gaacagctgc ccggcgtttc ggaatattat gacaccctgg
     1921 atgcgctgat tgcacccagc cgtgtcagcg ggcgggtgac tgcacagctg gaaatggcac
     1981 tgcccggcga gttccgggca gaaaatgtcg agccgctgag aaagactgcc tttttcaagg
     2041 cggcagaggc agacggctat gagatcgtga tggataccag aggcacgctg ctgtggcagg
     2101 aggatgcggc gagcgagcgt ccgctgctca tcgtcaccag cgaggcggtc agcggcgcat
     2161 atctgcgcta tctggaggaa aaccacattt cgtggatcgc ctgcggaaag gagaccattg
     2221 acctgccccg ggtgtgcggc attctggcgg aacagttcgg cgtcaagcgg atggcagtgg
     2281 tcggcggcgg tcacatcaac gccggattcc tggcggcagg actgctggat gaggtgagcg
     2341 ttctggtggg cgcaggcatt gacggcagag gcggcatgac ggcggtcttt gacgggctgc
     2401 cggcagaccg tccggtcaca ccgctgaagc tgtcggaggt caagcctttt gcaagcggcg
     2461 ctgtgtggtt gcggtatgct gtgatctgag caaaacagcg cttgcaggaa tttctgtggt
     2521 ttttttcgaa aaacacttgc aatttctgta agagtatgct ataatacaat tatctgatgc
     2581 gttgacatca ggacggattc cggaatatag gtgtgcgggt cctgtgcaac aaagcaccgt
     2641 gaaccatgtc aggcggggga ccgagcagca ttaagcggat gtgtttgtgt gccgcagcaa
     2701 acctgcacac ctatgttccg gaattttttt gacgcaaaaa tgcgtttctg ctgggaggtg
     2761 taagggttgt atcaggcatt gtatcggaag tggcggccga ggtcgtttga cgatgtgatc
     2821 tcacagccgc acatcaccac agcgctccgn nnnnnnnnnc tacctcttta ccggctcccg
     2881 tggtacagga aagaccacct gcgcccgtat cctggcaaag acggtgaact gcgaacaccg
     2941 caccccggaa gggaatccct gtctggaatg cgagatctgc cgtgacgcag agcgtggggc
     3001 gctggcggac atcatcgaga tcgacgcagc gtccaacaac agcgtggagg acatccggga
     3061 tctccgggaa ggtacggtgt atctgccgga gcgctgccgc tataaggtgt atatcatcga
     3121 tgaggtgcat atgctgtctc ccagcgcctg gggcgctctg ctgaaggtca tggaagagcc
     3181 gccggaatac gtgaagttta tcctggcgac cacggaggtc cacaaagtgc cggcgaccat
     3241 catttcacgc tgccagcgct atgacttcca ccgcatccgt actgctgaca ttgcggcacg
     3301 gctcacctat attgccggcg aggagaacct ttctctcgac ccggcagcgg cgcagctgat
     3361 cgcccatctg tcggacggcg gtatgcgtga tgccctgtct ctgctggatc agtgtgcatc
     3421 cggcggcgac gccatcacgg cggcatcggt cacagcggct gccggagttg ccggacggga
     3481 cagcgtattt gccattctgg aggctgtcca ggcacaggat gcggcaaagg cgctggagat
     3541 caccggggcg ctctacgacc agtccaagga catggtgcgg ctctgcgacg aactgctgga
     3601 acagctgcga aacatgatgc tgctgaaatc cgggtcggga cagccggaac tgctgacctg
     3661 tctcccggac gagcagcagc ggctggaaga gctgacccgt cagccgtccc tggataccat
     3721 cctgcggcag atcgcactgt tacaggactg ccgggagcgg atgcagcgca atccgggcaa
     3781 gcgtgtggaa ctggaaatgg cgttgttaca gcttgccgtg ccgcatctgg ctgccccggt
     3841 gcagaccgct ccggtacagg ctgctgcgcc gtctgcaccg caggtacagc cggctgtgcc
     3901 ggaaacgcct gccgcccagc cggcagaaag cgaatctccg gcggcggaat ccaagccgct
     3961 gaaagcctcg gacttcaagc cggtgggcaa ctgggcagac attctggcag cataccaggc
     4021 agtcaacccg gcggtctccg gcagtctggc ggaatcctcg gcgtttgtgg cagggaatac
     4081 catgctgatc gtggcgaaaa accggttttt cctcacgctg ctgaaaagca aggaaaacgc
     4141 cgtttcgctc cgggagactg tcaagcagtt cctgggcgtg gatatctaag tggcgcgaaa
     4201 agggacagtc ccctttcgcg ccattcgtga gcgtatcgaa ggcgtgtcaa atcacccgtc
     4261 cctttgacac gctttgacgc gctt
//
LOCUS       DAWENO010000179         4252 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7486_length_4252_cov_8.988087, whole genome shotgun sequence.
ACCESSION   DAWENO010000179 DAWENO010000000
VERSION     DAWENO010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_4252_cov_8.988087"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2102..2111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          980 a         1131 c         1103 g         1028 t
ORIGIN      
        1 acgcacgtcc gtatcgccgt gccaaggttg gagtcaatgt cctggattcc cacttttccc
       61 agaatcgaag tcagactggc aaacacagca gaaaggatgg catagaacag ccatcctttt
      121 ttagaagaaa cggttttcag atcgccgctg tgtttgggca gcatgaggaa tgttcctgcg
      181 ccgatgagca gcatacagca gagtttcagc catgtcagcg actctcccag caagaggaac
      241 gccagcagca ttgtgagaat ggtgctggat ttgtcgatgg gagcgacctg gctcacatcg
      301 cccagttgca gtgccttgaa gtagcacagc cagcttgcgc ccgtggcaca gccggaaagc
      361 agtaggaaca ccagcgtctt tcccgatatg gaaccgattc cggagaatgc cccggtcagc
      421 agtaccatca gccaggaaaa cagcagtaca atgatcgtcc gcagcgctgt agcaatggtg
      481 gaattgatgt gctgcatccc gatttttgcc agaactgcgg tcacgccggc aaacagggca
      541 gaaccgcagg caaaaagtaa ccacatgtag aattcatctc ctaatcaggc atgaaaacgg
      601 tagcctgcac cccatatcgt ttcaatatat ttcggatggg atggatcttc ctccagcttt
      661 tcccgcaggc ggttgatatg tacggcaacc gtagcattgt cgcccagcgc atccatgccc
      721 cagatgcgtt cgtataaggt ctcccggtcg aataccgtgt ccgcattggt caccaggaac
      781 agcagcagtt catactcttt gtttttcaga ttgatctcct gttcctggac gaatacccgg
      841 tgggacttgg gacagatccg gattgcgccc accgtgatat cctgctgtac ctcctggggt
      901 gctttcagcc gtttgtaggt ggcaatgtgt gcctgcactc ttgccacaag ttcactgggg
      961 gagaagggct tgacaatgta atcatctgcc cccagaccca atccccggat tttatcgatg
     1021 tcctcttttt ttgctgtcac cataaggatc gggatatcca gccgttcccg tagctttcgg
     1081 cacagtgtga acccgtccag ctgcggcagc atcagatcca gcaaaatcag atcgaacgat
     1141 tccttttggg tcagattcaa tccggagaca ccgtctgtgg caatggatac ggtaaagccg
     1201 ttgatttcca gatagtcccg ttcgatttct gcaatggcag catcgtcttc aataatacag
     1261 atatggctca tggtttcgcc tcctcctttg gaatggtcag cagaatccgc agtcccttgg
     1321 gatgtacatt ctccgcctga acggaaccgc cgaaccgttc cagaattttt gccgtaatgg
     1381 cgagtcccag tccgctgcct ttctgcggat tgctccggga cgggtcgctg cgatagaaca
     1441 catcaaacag cttggacagc gcctcttccg gcacacccgg accgttgtcc gttacggaaa
     1501 tcgtagcatc cgatgacgtc tccgtacagc cgatgcgcac ctggacttcc ggatcatcct
     1561 tgtacttcaa gctgttttcc agcagattga ggattgcatt gcgaaactgt accggatcgg
     1621 cgcagacaac acagtcagtg ggaatgtcgt ccagcacaac tgtcatgccc tgtctggcgt
     1681 attcttccgc attggcggca accagactgt gcagcgcctc tgcaacagga atcggctccg
     1741 gatagaacgg gttatctccc agatccagct tggagaagag gaacagcttg tctaccatgc
     1801 ggtcgatgtc gtttgccttt tcccggatgg tctgcatata tttctgctga cgctccggcg
     1861 tctgtgcaac gccctcgatc agtccctcaa tgtaggcttt gatagaggtc agcggtgtcc
     1921 gcaggtcgtg agagatgccg gcgatcagtt ccttgcggtt tcgttcttcc cgttccgtct
     1981 gcacaatgga ctgctgcaag cgttctgcca tctggttgaa gtcctgacag accggaagaa
     2041 actcatcttt gttggaatag cggatgcggt atgccagatt gccgtcctgt atctcatgta
     2101 cnnnnnnnnn naatatccag aggcttttcg attttccgga acacaaactt tgtcagaaac
     2161 cggtttgtga ccaggactgc gattacaatt aaaataatca aaacggcgat cagatatttt
     2221 acgatcattt cgttccaggt gctttcctgt accggcgcaa agaaagcaat gtagatggtg
     2281 tagttatggt catctatggt gatcggttcg acatacacat tgccgttgga aagcatatac
     2341 agcggcattt ccgccagatc ttcgtgccgg tcgcccatgg aacaaaccag ggcatctccg
     2401 tcgtaaatct gtagctgaat gctctgggtc tgcacatact tttctacggt ttgatacagg
     2461 tgagaatccg taagcgcctt atgatcggtt tgcagaaact cctctgcctg ctttaccagg
     2521 agatgttcca gatacacggt attttctcca cgaaccgttt catccatggc ctgcttgtag
     2581 aaaagattca caaacagcaa catggaaagc gccaggatca aaacgctgac cagcgccggg
     2641 atcagcagca taaagaggtt cgaaagaaac agccgctttt tgattgtcaa tctgcacagc
     2701 tcctttccag gtgtgttacc ttacacttca ttatagcaca ttacacttca ttcgtcaatt
     2761 tccgccttgt cccggaaaat tgtctttgcc agggtacgct ccgtcagaat cgccgcaata
     2821 gcaaggagaa caaagaacag caaagttaca ggagtgggga cagccagatg caggatgtcc
     2881 agatggagca ggtgattttg cagccacagg caaacagcaa tggcggcata gaatccaatc
     2941 gcagaggtca cagcaaaaaa cgttttgatt ttggtgaaag gcaggcacat tttgaaaaca
     3001 ccggcaagcc cgactgtacc gatcagcagg aacagcagtg catttgcctg ggtctgttca
     3061 ccggacagtc cgaataagcc cattgcgtgg aatagcagat agaacaggaa gcatacacca
     3121 atggcaatgg cattcggtgc ggctcgccgg atcgcctccg gcagaaaccg ggtgcgcacc
     3181 ggacgggaat ccggcaggaa agacaggaaa aaggacggat acccctcgat aatcaggtcg
     3241 atcagcgtga tctggatggg aacaaagggg aatggggtat tggtcagcag acaaatggca
     3301 cagagcagga cggagtacag cgttttaatg aaaaagacgc ccgcagatcg tgtgacattg
     3361 tggaccactc gtctgccctc cagcaacacg tctttcagta cggcgaagtc ggagtccagc
     3421 agtaccagct gtgctgtctg ccgtgcggca tcgctgccct gtcccacgga aatgctgcag
     3481 tctgcctctt tcagtgccaa taggtcgttt acgccgtcgc ctgtcatagc gactgtgtgc
     3541 ttctgtcggt ggaatgcctg tatcagctgt tttttttgct gcggcgttac tctgccgaaa
     3601 acggtatatc gctgtgctgc tgcgtcgatc tcagattcct ctacagttcg catatccacc
     3661 cagcggtctc cgtgcaagat gcctgccttc tgtgcgacag ccgaggcagt gaccggattg
     3721 tcaccggaga tcagcttgac ggaaacgccc tctttctgga aatttgcaat tgttttcgcc
     3781 gcattggcac ggagcggatc ggtcagcacg atcactgcca gcagttgtac ctccggcagg
     3841 ggctgccctt tctgtaccgg ctgctgtgtg atgcctgcca gcaggacacg cttgccttcc
     3901 ttttggtatg cggtgatctc cggcggcatc tgatccggac aaagcttttc cggtgcgccg
     3961 gtcacaaaag ttcctgcgcc ggcaaaggtc atggcgctcc atttccgatc cgaagaaaac
     4021 gggatctgat cggtgacact cagcgtatcc atacccttga aataggcgtg catcgcctgg
     4081 aatgtggcgt tgttgtcctg tgtgtgtgtc agaaaatttc ccatccactc gtggaatttt
     4141 tctgcgtccg ttcccggaaa caggtcggct ttttccacct gcattttgcc ctcggtcagc
     4201 gtaccggttt tgtccaggca aagcacatcc acgtgcgcca gattttccag gg
//
LOCUS       DAWENO010000180         4219 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7553_length_4219_cov_10.701729, whole genome shotgun sequence.
ACCESSION   DAWENO010000180 DAWENO010000000
VERSION     DAWENO010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7553_length_4219_cov_10.701729"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    596..605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          847 a         1120 c          944 g         1298 t
ORIGIN      
        1 atattcactt ttctactttt ccacgctgac cgttacacct gatttgaacc ggaactccaa
       61 atagtcatcg tacacagtca ctttttcaag cagcttcttt acaagactgc cgtcaaacac
      121 taaaaccttg ctcttctgcc gttctataaa tttcagcagt tcccggatac gcttcttgtg
      181 ttcagttttt gcgttgtcgt ccattgctgt ctgttcctga agttcccgca ggcgcaggat
      241 ttcttctgcc agatcgtcgt agttcgtgcc gctttccgtc cggtcgatca gctcctgctg
      301 caattgccgc atcctttccg caagggcttc cgctgactgc ggattagcaa ggctgatcgc
      361 agttttcaga ttgcttttca gctttttcag gtattcgtcg ctgttgccga gcatttcatt
      421 gaccgcgtca ataaaccctt gttgcagttc atcctcgctg accgtccgtg ccgagcagcg
      481 tgttttgtcc tccagtctgg tcttgcaccg ccagacgatt gttttcttgc cccggttgtt
      541 ccagtgcagc ctgcggaaat gctcaccaca gcactcgcag gtcaccatgt gcgagnnnnn
      601 nnnnncgaga aggcgtggtt tgcgctgaat ccctgccgcc ttccgttcag atcacgctcg
      661 gatgaccgcc gcaccatttc ctcctgcact ctcatgaata tatccttcgg gatgatcgct
      721 tcgtggtcgt cctcaatgta gtactgcggc agtgtgccgt tgttcttgcc ccgtttctta
      781 ttcagaaagt caatggtgta ggtcttttgc aggagtgcat cgcccatata cttctcgttc
      841 tccagtattt tgcggacagt gctgtcgtgc cagcgtggat tgtcccgtgc agtgcgaatg
      901 ccgtcacgtt ccagccctct ggcgattttc atacagcttg caccctccag atactcccgg
      961 aaaatgcgct tgacgatctc cgcctccacc ggattgataa tcagatgccc ctcctcgtcc
     1021 ttgtcatagc cgaggaagtt tcgtgtattg accatcactt ttccctgctg gaaacggtac
     1081 tggattccca ttttcgtatt cttgctgaga gattctgatt cctgctgtgc cagcgatgcc
     1141 ataatggtaa tcaggacttc gccctttgca tccatcgtgt tgatggactc cttttcaaag
     1201 aaaaccggaa tgttgatctc cttcagcagg cggatatatt tcagacagtc cagcgtattg
     1261 cgggcaaatc ggctaattga tttcgtgaag atcatgtcga tttttcctgc cttgcagtcc
     1321 tcgatcatag cgttgaactg ctctctgttt ttggttgagg tcgcactgat accttcatca
     1381 gcgtacactc cgacaaattc ccactccgga ttcgtcttga taaattcctc ataatgctga
     1441 atctgtgcct catagctgga tgcctgttcc tcgttgtctg tgctgacacg gcagtatgct
     1501 gctacccgca gacgctgtac ttcttctttc gccgccgaat tgcctttctg cggtttcgac
     1561 ggaatgacgg tcatgttttt catgatttca cctctatcag acgatatatg tattcggctt
     1621 gctgcaccgg atcgttaaaa tgcgtttccg cctcagtcag gtcgaacact ttgtgaatcg
     1681 gcggtgcggt ataccgcttt tcatggctgt gctgattcgc acgatggatt cttatgccgt
     1741 ttgcttttgc aaatgtctcc cggctgataa tcgcagggta gtagtcggtg ccgatgtaga
     1801 tgttctgcat caggattctc ttgacagtgc tgtgctgcat gagaacgccc gcattcgccg
     1861 cagccgcctt catgctcagc ccggaaacat agccgttgaa aatgttgacg atgacgccgg
     1921 cttcctgttc atcgatgacg gctttgccgt ttatgatttt gtatccgtac atagtgcctc
     1981 cctcaatgtt agtccgcatt tcagcttgaa gccgacgctg tttctgttgt aaacgatgat
     2041 gctgtcaaca aaagaagtga ataactcctc gttgtaagcg tccaacatat ccgctgcttc
     2101 caaaaaatgc agcagttttt cggtttcccg cagcatcaga ccggactcaa tattgccaag
     2161 gctctggatc tctctgcggg attcctcgtt ctgcttttcc atgcggttca gttcctgatt
     2221 gtacatcact gcgtcaatga tgctctttac ccggagcttt ttcaactcgc attttcggtc
     2281 gttgtttttc tggatgctcg ctttcagaca catgatacgc tggagatttt catcgctgcc
     2341 tgccacacgc agggcattgt aatacgggcg gaacagtatc tttcgggtga atatgagctt
     2401 gttcagcatc gtggtgaaag cggactggaa tgcctcctca tggataaatt tcatcgagca
     2461 gctatcctta tccgcaatgt gcttcttgca gcaccagctg attccgctgg agattacctg
     2521 acgcttgaat gtgctgccgc acgcaccgca gatgattttg ctgctgaaag cataccgtcg
     2581 ctggtacttt tcatcctcgc gccggatacc gcgttccctg agccgctgtt ccagaacatc
     2641 atgtgttgtt gcgtatagct cccggctaat gattgcctta tgatgatctt tcacttccgg
     2701 gctttccacg tcgcccttgt tcctgtgcct gcgaaagctg tcgtccgtat aggtcttgtt
     2761 gtagcggaca tcaccgacat atttctcatt attcagaatg cccatgaccg ttgtggtact
     2821 ccacttgccg cctttacgcg tcggcacaca ccgcctgtcc agttctttgc agatcttatg
     2881 cgttcccgtg ccggaaacaa cggacttgaa aatgaagcgg acaatgcctg cttcatccgg
     2941 aatgatcgta tagtttccgt cactgtcgaa ggtgtagccg taaggcgctg ttcctgctct
     3001 gtacctgccg tttttgatgc gtttctggtt gccccacttt acatttttgg aaatggactc
     3061 ggattcctcc tgtgccagac tgctcaggat tgcaagcaca agctctgttt ccatacttcc
     3121 ggtatccaga ttctcttttt caaagtaaat aggtaccttg taagaaagca gctcacgcac
     3181 cagttcaagg cagtccgtgg tgttgcggga gaaccggctg acggatttcg tcagcacata
     3241 gtcgattcga ccgatgcggc actcgtatag cagtgcctgt aagccgtcac gaatatctgt
     3301 ctttgtgcca gtgatgccga aatcatagaa tacgccagcg tactcccagt cggaatgcag
     3361 cctgatccac gcttcataat gtgccttctg cgtttccaga ctttcccgct ggtcgttgtt
     3421 atccgtactg acacggcagt aggcagccac acggagtttc tttttctcag ctggctgtgc
     3481 ctcaatggtt ctgactgtca tcgctttccc tccttgtcag tatcgtatct taactctgat
     3541 tcgatgattt ttcaagtggt ttcggcaata agtctgcata taggggagag aatgttttcc
     3601 ggttcagatc ggttaatttg tcatattcgt caaatgtaat cttcccgctg gtatagagca
     3661 gttccgtgat tgcctgtgcc ttgtagtatt cgatctcatc catgattttc tgctgatcca
     3721 tatggacacc tcctattgac agccggaaaa gtcaaccccc tccggatttt tcacaatcaa
     3781 taggacactt ttttggattt tagcaaaaga aaatgcccct tccgagagaa aattctccca
     3841 gaaggggcat agcattaaat atccttctga ttcacccaac cagtgacgta gcttccgaca
     3901 ggtgtttttc tgcagtttgt agcagaattt gtgatacgca ttctgccatt taccacttgt
     3961 ccatcataga gatagaatgt accgcttttc tttgttccgg tttttgatat actggacgca
     4021 tacagggtgg cattgttcag tatgatcttc gtcccctttg catatgtctt ttcagctacg
     4081 gcatatacca ctcttcctga attgtcaaaa acgctatagc ctgtcttgca cactttcttc
     4141 gcattctcca aagaggaata cgcaccaatc tgcgacttag catcagccca tgtttttctt
     4201 actcgataaa gctgttttg
//
LOCUS       DAWENO010000181         4183 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7615_length_4183_cov_10.581395, whole genome shotgun sequence.
ACCESSION   DAWENO010000181 DAWENO010000000
VERSION     DAWENO010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7615_length_4183_cov_10.581395"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    520..529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2886..2895
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1067 a         1062 c         1180 g          854 t
ORIGIN      
        1 tttgtgtggt gttgccggaa attagaaata aggagaatac cagatgaatt gtaagaattg
       61 tggaactgcc gtagaagacg gcaagaagtt ttgcccgaac tgcggtacac cgctgacaga
      121 agacaccccg accccgatgc cggaggctgt ccccgatgca gttccggata tgccggaaac
      181 agctcccatc ccggatgcgc cggaacagat gccggacgca gcaccggaag caccgcagcc
      241 tgcgccggag accagcgctc cccagagcca ggctgcaccc caggctcagc cccagcagcc
      301 tgtcggcgga gaaaatccct atgcgatgcc gggcaacacc attccgggcg gcgcaggcgt
      361 accgccggtg aacggaacac cctatgaccc caccaccaat cccattccgc ctatgggtca
      421 gcagcccaag aagaaaaaga agaccggtct cattgtggcg atcattctga tcccgattct
      481 gattattctg attatcattg gcgtgatcgt gggcatcggn nnnnnnnnng gcatcggcgt
      541 aaacatgggt accaagggcg caaagaccgt agacaaatac tgggatgccc acgtgaactg
      601 cgacgcagag gcgctggcgg atctctgccc ggacgatttc tggaactata tttccgacac
      661 ctatgatctg accgaggaag aggcagtgga tgccatgggc agatacatga acgactatgc
      721 cgacagcctg ggcggtgacc tgtcctacga gtggaagcag tccggcgtgg ttgtgggctt
      781 cggcagcagc tcccagctgg acactgtccg ggaagatacc gacaagtttg gtctgaaggt
      841 ctcctccggc atctgtgtgg atgtaaactg caccgttacc ggcgcagacg attccgagca
      901 ggacgactat tccatctgga ccgtcaaggt gaacggtcag tggtgcagcc tgtccgcaat
      961 ggatgacttc aacgagatct gcgactccga ctatgccgca accgcaaagt acggcgcaga
     1021 gttcggcgat atgctccaga cctactggac cgctgttgcc aatgcagacg cagagacact
     1081 gtccaccctg gttcccgacg gctggtggga tctggtagac caggaatacg gcgtacagca
     1141 gagcgatgcc gagagctatc tgaacagcct gctggaagag atggtggcat ctcagttcaa
     1201 cggcgaatcg gtggacgata tccaggtaga gatcaccgat gcaagctctt atgacgacga
     1261 tgaactggca gacatcaacg agggtctgga cacctatggc attgacggcg aagaggcaat
     1321 ggacgtagac gttacggtaa ctaccggtga cgacagcaac gacacttatg taacattaac
     1381 caagatagac ggtcagtggt atgtctatga tgcgatgtac tatttcgcaa ctgcctgcta
     1441 cggcacaagc cagggcggaa tgtaaggact tttccgcact ttctgccgtc acagcaaagg
     1501 agtaaacaaa gaacatgaaa tgtgaacact gtggatatga cgcaccggag cgtgcggtga
     1561 tctgcccgga atgcggcgag atcctgccac gccccaaaaa agcggaggaa gaggagaccg
     1621 aaaaggcggc tgtccagaaa aagacagccc cggacgccga ttctttctcc atgccctttc
     1681 tctctgcgcc ggctgacctg gaaaaggcgg acggcatcgc cgccgaggac ccggaagtgc
     1741 tgcggcggca aaagcaggaa aagaccaccc gtctgacacg ccgcatcgtg gcggcagccg
     1801 tggcagcggc ggtggtcctg gcggcggtgc tgtactacgt ctttttcggc ggctataagc
     1861 tggcggtata ccgctatgtc aagggcgtgg accgggagag cggcagtatg tacgtggcgc
     1921 tggtgccgga tgcctatatg gactatctgg agaccaccta ttccaccaca cgccgggaag
     1981 tcaaatccat gatgcaggat tattttaagt actggaacaa aaactacggg acagacggca
     2041 gaatgtccta tgatatcgga aagaccgagg agatcacaga cgcagacgag atcagcgctc
     2101 tggaatcgga actcaagaca gactataaca tcaccgtgga gatcaaaaag gcagtgaccg
     2161 tcagcgtcaa tatcgacgac ggcggcacaa aggcatccga gcaggcgacc ttcgtcaaga
     2221 tcggaaaccg ctggtgctgt atgaaggcga tggaggacat cgactttgtg tgccagaatg
     2281 acggctataa cgtctggtaa tttcggagag gacagaggag caaaccaagc atggcgaaaa
     2341 aagtgacaaa aaatcggaaa caggcagagc aggcggcggc aaagcgccgg catctgcgca
     2401 tcgtggcagg catcatcatg gtggcagcgg cggctgggct tgcctgcggc ggactggtct
     2461 ggtatcagca cgaccagaaa gcacagcagc aggcaccgga ttccgcagat tatgccgatg
     2521 tgctgcaatc ctattatacc gcagtctttg cggcggacgg tcagacgctg acccagctga
     2581 tggcgccgcc ggaatactgg acgtattaca tggagaccta taacaagacg gaggaagacg
     2641 tgatcgacag cttcgcagag ggctgcagca acaccgtttc ggagtggaaa gaaacctacg
     2701 gggacgatgt gaaggtgacc tatcagattg agggcttgtc ggagcagggc gattccggcg
     2761 tttcggagtg gaactccaac atggaagaga tgctggggaa cgacggcgct gacatctccc
     2821 aggcggtaac gctggaggta cagcggaagt tccagggcag caacgattcc ggcacagacg
     2881 gcgtcnnnnn nnnnnatccc accctgggca agatcggcga aaagtggtac atcatctctg
     2941 aggatgacga taccctgaaa agcggcgctg tacagacgac agtgcagtaa cagcgattac
     3001 agcaacacca tacaaacaaa aaacagctat cccacaccag gatagctgct ttttgtttgc
     3061 agtgctgtga ttttctctta ctttctgtgg aacagaccct ttttctcata gggttcgctc
     3121 tcgtgggaaa gcattgtgta gccgatgccc ttgatctcct tgtccagcat cgaaatatcc
     3181 agcggctctt cgctgatgac tacggtctca ccatttttcg cagaagaagt cacctttttt
     3241 accttgcagt tctggcgaac caggtcattc atatgtgcct cgcacatact gcacatcata
     3301 ccgtcgatct ttaatgttgt ctttaccatt gctcgttacc gcctttcatg tgatagactg
     3361 ctttgcagta cgtctctatt atatacccta taggggtatt tgtcaagggg tatttttcga
     3421 atttcatggg aattgtgggg aaacaccgca ggagatgtca gcaacactgt aggggcgaac
     3481 atggttcgcc cgagagactt ccgactcctc tgcccttcgg gcatctcctc tacgagagga
     3541 gactattcgg tatattgtcc cccttgaaaa gggggatgtc ccgaaggggc agggggatca
     3601 acccctacaa atctcgcctc accaccccac acatcgtgca tctcacggga gtatcctgcg
     3661 aaaatacgat tttgataatg cggatattgc cttgcacaat atttgatgcg caacattctg
     3721 cttgtttgca cgatatgtgc ataaaatgag caaaatttgt ggttgcattg gacggaaaat
     3781 catgctataa tagaggcaat gcaattgtac ttgaaatgga tctgtctcgg attttcctac
     3841 tgttctccgg aaaaagacgg ggcgatccga cgaacgcaaa ggagaacatc atgaaaattg
     3901 gagttatcgg acacaactat acccacggtg cagattttgt gatggattat ccggacggac
     3961 cgggctgtta tctgttcctt ttggtgaaaa cacctgcacg attgctattg aacgggaaaa
     4021 agttgcatct ggaaaagaat acaattctgt tttttacgcc ccacgctacc tgccgctatg
     4081 aggcggagag cggtgtctat acggacgact ggttttactg caatatgacg ccggaagaag
     4141 agatcgccct gcgcaatgag ggaatgccct ttgatacgcc ggt
//
LOCUS       DAWENO010000182         4179 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7624_length_4179_cov_7.929437, whole genome shotgun sequence.
ACCESSION   DAWENO010000182 DAWENO010000000
VERSION     DAWENO010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_4179_cov_7.929437"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          920 a         1088 c         1114 g         1057 t
ORIGIN      
        1 cgacaggtgg ggcagccgat ggagttgatg ttcagcacga tgttcttgag tccggcacgg
       61 tcgagaatgg tctttgccag cgcaatgatc tcagcgtcag aagtcggtcc ctggctgcct
      121 gccatttcga tgccgaactg gtggaactcc cggagtctgc ctgcctgcgg acgctcgtgg
      181 cggaaacagg acaggatata atataccttc tggggaagtg catcgttgag cagaccgttc
      241 tggagtatgg accggatcgt gccggctgtt ccctccggac gcagggtgaa ctcggtttcc
      301 tttgccttga cggtatacat ttctttctgt accacatctg tggtttcgcc cacagaacgc
      361 aggaacaggt cggtgctttc aaaggtgggg atgcgtgttt cgtgaaagcc gaaattctcc
      421 gctgtttccc ttgcaatgcg ttccaccgta tgccacttgt agatctgctt gggtgtcaca
      481 tcttctgttc cctggggacg tgtgattttg attgccatgt atgaaaaacc tcctcgcact
      541 ccggagaccg gactgcaaaa aatttcccct tccgaaaagg ggagatagtt gtacaaaaac
      601 ttgacggcag ccctgtgctg ccagaaatgc ggtgtgctta aatggtcgga ttgccgattt
      661 cccgccagtc caaatcgcca cgctccagcg ccatgatgag cgcctcagcc gttgcaatat
      721 tggttgcgac cggaacatta tgtacgtcgc agagacggag cagattcatt tcgttcggtt
      781 ccgatgcctt gcggttgatc gggtcacgga agaacaggag tacatcgatc tcgttgcagg
      841 agatgcgtgc tgcgatctgc tggtcgccgc cctgagaacc gctcagatac cgctggatcc
      901 gcagaccagt cgcctctgcc acctgcttgc cggtcgttcc cgttgcacat agggtgtgcc
      961 gggagaggat gccgcagtag gcgatacaga attggatcat cagttctttt tttgcgtcgt
     1021 gtgcaatcag tgcaattgtc atgtcagata accattccct tctacattct ttatcgtatc
     1081 aaaaatgaaa cttagatatt cgttctatga tttcattgtt ttcagatagt tagaccacta
     1141 catcatttat tatacaccat atcccggtat ttgtcaaaga atttccgtga gaaaaatata
     1201 aaattgtgaa ttgtatggtg cttcccacgt tcacccttgt gcattctgac gaattctgct
     1261 tctcagtctc ttgcggtggt gcttgttgta gaagttgtgg tttccgtacc ggtcgtatcg
     1321 gtggatgcgg tcgttgcgcc caccatgtct acattggtct gttcgccctt gacagttttt
     1381 tcgtatgcct ggatgatcga acggatcatg tacttggagt attcgccgcc ttcgatcaca
     1441 ccggcgaaag cgatttccgg atcatctgcc ggggcatagc cgatgaagaa agagtcctgg
     1501 actctgccgc tgacctgggg cgtaccggtc ttgactgcca catcaaatcc caggtttgac
     1561 agggaatact gcgccgggaa attgttggtg gatgcggcga tcataccgtt ttccacatac
     1621 tggaatacgc tgtcgttgag cggtgtgatc tgctctgcca ccgcaggctc tttctgttcc
     1681 agttttgtgg tgtggctgta gtcccagatg ctgtccacca gatgcggctc atagcgtgtg
     1741 ccgttgttgg cgatggtgca ggcaacattc gccatttgca gcggcgttac agcccattcg
     1801 ccctgaccga tcgcactttg cagtacctga ccgacggtcc agtctgcgcc cagttccgca
     1861 aaggtctcct ggttggacag atgtccggcg gcatcgccgg tttccagccc ggtggactga
     1921 cccagtccaa atttggaggc atactctgta atgcggtcga tggtcagttc ctccgacagc
     1981 ttgtagaaga agatgttgca ggatacccga agtgcctgtg taactgcaat gtcaccatgt
     2041 gaaccggtac agttgtaatc gtggtctttg aaatgatagg tgtgtccgca gaaataggtg
     2101 ctgtttccgg tgatgatccc ctcattcaaa ccggcagttg ccgtgatggt cttaaaggtg
     2161 gaacccgggc ggtagagtcc gtctgtcgcc cggttgacca gcggcgaatt ttccgcattc
     2221 agaatggatt cataatcagt cttataatct tccaggttgt atgtcggcgc tgtgaccatg
     2281 cccagaacgc cggcagtttt ggcatccagc acaacgatcg caccgcaggt gacctttttc
     2341 agccccagtt cttccgtctt ggaattccgc aggctgtcaa aattattgat aaagccgtcc
     2401 agaatgtttt gcagttcccg ctgatagtcg ctgttgacag tcagctgaac cgtcttgcca
     2461 gcctccaccg gggtttccat gtccgaagaa acgacaacgc cgttttctac ggtgattttc
     2521 ttgatgccgt cagtgccacg cagggtgctt tccatggcac gttcaatgcc gctttttccc
     2581 acggaatcat ccagagcgta accctcagac ttcagctttt cgtattcctc cgcatagatg
     2641 ggaccgacag taccgatctc gtggggaatg acatcgccct gtgctacaga acgtatcgcc
     2701 tcttccacaa tgtccatgcc ggggagcgtg acgccacgtt ctttcagttc cgtgaccaca
     2761 ttcagcggca catcctctgc cagagtgaaa cggttgctga gggaaaagcc acgaagctgc
     2821 atctcatagc ggattccggc gagcagtcgc tgttcctccg gcgtatagct gtcgtcaatg
     2881 tcatagtcct ctttcagctt gtccatgcag ttgtccgctg tggcatagac attcatgttc
     2941 aggttttcct tgagcttgct gaccgctttg tcatcaccgg taaaggtgta tggctgtgta
     3001 tccgagatcg gcagggaagt gtcccattct acgccgtttt cgtccaggat gtttaccaga
     3061 gagagcagca tctggttgcc cttggcgttg tcctccggga aagtgtccgg ctcaatgata
     3121 acattatagc ctaccttgtt ttcgatgatg gctttgccgg aggaatccac gatctcgcca
     3181 cgggtggcgc tgatggtctg ggtgtattcc tgggtggaaa cccgctttgc ggtgtaggtg
     3241 tcgctttcca caatctgtat tttcatcagc cgccaggcac acgcacagct ggcaagcagt
     3301 accagcatac tggcgagcgt aagtttgaca tagctggatg ccgtatgttt catgaaatgc
     3361 tcctttccgg agaaaaacag ggcggcacag cacgggaaat ggtaaatttc cgatgctgtg
     3421 cagccgctgg tcacgccgat tcttccatag gcttatgctt tttctcaatg gtgcgccgcc
     3481 gcttcggcag cagaaaatgg tgaatcaaag caaaaatggg gtagatcacc acactgctga
     3541 gaacggtgta tcccagacag ggcagctggt ggtgcagata cagccgggag acattgtcat
     3601 atccccagat ggcatactgg aacacaaaat ccagtcctgt caccacaaag gaaaccgccg
     3661 ccgtcagaaa catcatgttc cagaaacgct ggagcagaaa ccgatcatag agccgggaag
     3721 tgaacaggca gatcaaaaac agcagaatcc catgatagcc cggcagtgtg ccgcaggaaa
     3781 tatccagcag cagaccgcac agaatgccga tgccgccgga gactacagtt ccggagacgg
     3841 atgcaatgca cagcgccacc gggatcagca gcaggggctt gataaaatcg ccgccgtttg
     3901 ccgtcacaaa agcgagaaaa ataaacagca cgtataagat ccagcgaatg gtattgcgca
     3961 gtctgggacg gcggacagac cgccgggaaa gctgctttcg cttagtttgc cgcatcctgc
     4021 tgctcctttc cggcgaaatc cttaatgacg atcacatttt tcagattttc cagatccaca
     4081 gacggacgaa taatggcaca atagctcaga ccattgtcca ttggctcgat ggattccaca
     4141 gaaccgatga gatagccctg ggggaaaatg cccacagaa
//
LOCUS       DAWENO010000183         4137 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7702_length_4137_cov_7.284664, whole genome shotgun sequence.
ACCESSION   DAWENO010000183 DAWENO010000000
VERSION     DAWENO010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7702_length_4137_cov_7.284664"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1079..1088
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1116 a          965 c          934 g         1112 t
ORIGIN      
        1 tcatttataa aataaacttc cggtcgctca cacgaccgga cgcaatgatc atataagatt
       61 ttgaatatcc cttctgctat aaaacatatg acgtacttcc atgatgttgc caataacaac
      121 atatagaata gtataattct ttacatatat ccgatagtag ggatattgtc tctgtttgcc
      181 tgaacggact ttttcaaagg cttccggcat ttcaagcctg tcgtatattg ctttctgcac
      241 ctctgatata aggagctccg ctgcttgtgg attttgtagc acattctgaa tgtagctggt
      301 acattcgtaa aggttttatt caaactccgg caggaaacgc aattcatact ttgccattat
      361 taagcctctt ccttacattt gaaaacactt catctcctgt cagccgcaca cttgtttcct
      421 cggcggcttt gtcggcacgg tcaaggtgca tttcaagatt ttcagtaaga ctttcatatg
      481 cctcaagact taacacaacc atggtaccat agccgttttt ggtaaggtac acaggattgc
      541 cgttagcaac tatattttct atttccggat atttattgcg taaatcggaa accggtctga
      601 tctggatcat aatgaaactc ctttcataaa actgctgttc cttactgaaa ttttatcata
      661 attttatcag aatgtcaata gcaaaattta attacggatt caccgctatg gtatcataac
      721 agtctgccgg aagtccaaaa aaccacgaaa tatacgaaca gcgctgcctg taccatttcg
      781 ttgaccctca atgcataaaa aacaagccca ggacctcccc gggcttaaat tcattccgac
      841 gtatcatcgt ccccacgcag cctgcgcagt atatcagcgc cgctcgtgtc ctcaggctct
      901 atgtcacggc tgagcctgaa agtcagcata tccggagcag aaatggactc ctccctgttg
      961 gttacactgc cggactgccg gtcggttatc tttatgtgcg gaaacaggtt gaatatcaca
     1021 actatgcggt tcggataaac cacaacatcg tgaatgtagg tatctacgag ccgcttcgnn
     1081 nnnnnnnncg tcagtgccct gcgtatttcg gaaaaagcgc atttcagttc tctttcggtg
     1141 acacgattct ctgaaagcag cttggttagg gcagtgatgt ccttttccag cgccgctttc
     1201 tgtttttcca ggtcggacag tttctttttg agcgaagctg aaccgacatc tatcagcagt
     1261 tcaactgtgc ggttgattct ctgctccacc tccctcaggc tgctctgaca ctgcgccagc
     1321 cgctgtgctg aagagccgac acggttgtta agatactgat tgtagctttc cgttaatttc
     1381 ggaaccatgc catcggtaaa gacatactca gccagcttcc ccagcacaaa gctctcgata
     1441 accgagcggt tgacctgcgg attgctgcac cgctcgtcct tggcatgatt gcggctgtta
     1501 cagaagtacg agtaggacga tacccttgcc gtttgtcttt ctctccccgc agtaggctct
     1561 gccgcacaaa ccgcagtgga tcttgccgct cagcaggtat acttccttcg ggtgagcctg
     1621 gttgcttatc cggcggcgct tcattctttc ctggatacgg ttgaactgtt cacggtctat
     1681 cagcggcggg tatacgtcgt cacgcactat ccactcggat ttgtccttga acttgcggct
     1741 gttgttcgca acggactgcg aaactcgcct gttgtaaacg catataccga tgtatttctc
     1801 gttcctgagt atatcgtgta cgctggtgct ggtgaacgtc tttcccatct tggtgcggaa
     1861 tccacgggat ttcagctctg cgagtatatc gggatatttc ttcccttcgt ctgccatgct
     1921 gaaaatcagc cggacagcgt tcgcctcatt ttcgtctatc tttatcttca tggtttccgg
     1981 gtcaacacgg tagccaagcg gcggcattcc tccagtatgc ttgccgttat aggcattctc
     2041 tttcagccct ttcatggtct ctctggcgag gttgcgggaa tagtattcat tcatgccctc
     2101 aatgacggac tggagaataa cgctttccgg tgagtcgtcg agattttcaa taacagaccg
     2161 caccgtgacc ccgtgctttt tcagctcgtg ccgataatac gccgagtcgt aacggtcacg
     2221 ggagaaccgg tcgagcttat gtacaagaac gacctgaaac cttccggatt ttgcgtcaga
     2281 tatcatctgc tgaaactccg ggcgctggtc agaacgagcg gagtacgccc tgtcaacgta
     2341 ctccgcaact atctcaatac cgttcctggc agcgaaatcc gatatagccc gctgctgtgc
     2401 gtcgatactc tcctcacgct ggttatcaga agagaatcgg gcgtatgaca cagctttaag
     2461 cggtgtgctt ggctggttcg gcatcaatct tatgatcaag gcacttgaag atcatgctta
     2521 cagcctggct ggggaaacag tggaggatca caaaagaaag tgtcgcaaaa tatatccggc
     2581 gaatggaata atatatcaaa agcaagggta acggcaagaa ataagtcact tacgaaattt
     2641 atgtataaat acgcattttt gtcgaaagga aattatcaat gtatattgac ttctgccccc
     2701 gggtatgata taatagaatc gatataattg tatcaatacg catttttgtc gaaagtggtg
     2761 taagcgatga tagaacgcaa gaagtacctt gacaagctta tcagccgaaa aaataacggc
     2821 atggtaaagg taataaccgg aatacgccgc tgcggtaaat cctacctgct gttcaatatt
     2881 tacaaggatc atctgaaatc gcagggcata agcgacgatc agatcgttga actggcactt
     2941 gatgaaatcg aaaatatccg gtacagaaat ccgattgaac tgggagagta cctcagtcag
     3001 aaaacctccg acgacagcaa gaattattat gtgttccttg acgaaattca aaaagtcgta
     3061 ccggtgcaaa atccatatat cgaaaacgcc gaagataaaa tcagctttgt tgacgtagtt
     3121 ctcgggctta tgaagcacga taacattgac gtttatgtga ccggcagtaa ttcaagaatg
     3181 ctgtcctcag atatattgac agaattccgt ggcagaggcg atgaaatacg agtctatccg
     3241 ctgtcgttca gcgagttcta taacgcatac gacggtgaca ggcataacgc atggcaggag
     3301 tacttcacat atggcggaat gccgtttatt atgcggaaaa agacccatga ggaaaaagcg
     3361 cagtatctac agaatctgtt cgacaaaatc tacatcagcg atattgtcga gcggaacaag
     3421 atacttcacg agaaatccgt acttgacgaa ctgctgaata taatctcatc ttcgataggt
     3481 tcgctgacta acccgagcaa tattgcaaaa acgttccaca gcgtccgtca gatcgatatt
     3541 gattccgcaa cggtagcgag atacctcgac ttcttcattg attctttcat gatttcaaag
     3601 gcggagcgct atgatgtcaa gggcagaaaa tacattggct cgccgctcaa atactatttc
     3661 agcgatgtgg gacttcgcaa tgcacggctg aatttccgcc agcaggagga aaaccacatc
     3721 atggagaaca tcatttacaa cgagctgcaa tatcgtgatt tcaacgttga tgtgggcgtt
     3781 gtggaataca accaccggga cgaatccgga aagagcgtcc gctccaggct cgaaatagac
     3841 tttgttgcaa gtaaaggcag caagaaatac tatgtgcagt cggcgctttc cgtttccgat
     3901 gaagagaagc gcctgcagga aactaactcg ctgtaccgtg tggcagactc gttcaaaaag
     3961 atagttgttg ttaaggacag gatcattccg tggcatgatg aaaagggtgt gctgtatata
     4021 ggtatcgagc agttcttgct tgatgaaacg tccatggatt tataaaacga attgggaatt
     4081 tactatgaat aagatcaaag aaacactgca tgaaaaaaga aaagcaacag aaaaagg
//
LOCUS       DAWENO010000184         4009 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7949_length_4009_cov_8.271877, whole genome shotgun sequence.
ACCESSION   DAWENO010000184 DAWENO010000000
VERSION     DAWENO010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_4009_cov_8.271877"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3198..3207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          754 a         1105 c         1028 g         1112 t
ORIGIN      
        1 tctgcactgc ccaggttctt ggtagcaccg caatatcccg gaccgccgtc tgcaacgctg
       61 tacgcatatt tcagcgcagc cttgccgttt ttcacgtttt ccttatccag tgtcagtgtc
      121 agcttgtcgc cgttggaatt ctgcttatac gccattgcaa cggcagtgtc atcgtcatag
      181 gattcaaagt catcaatgat gctccgtgca gcctcttccg attccttgct gtaaaccgtc
      241 agcttgactg tgggctggtt gccctcggtg gtcagcagct ttaccgtatg ggtgccgatc
      301 tccagctttg ccagagcgtc ctttgcaatg gtgatggtgt cttctgtcat ggtgtagctg
      361 tcagccggca gatctgcgtc atccagctgt aatccggacg ccttgccctg catcatctga
      421 taggacagtg taacgtcttc cggcgcattc aggttgaccc ggaggttctt tccggaaagg
      481 ctgttcttcg gtgtctggtt ctgcattctc tcagcgtgtg ccttgaaata ggtcagaatc
      541 gggtcgccgt ccatcacggt gaattcgctg agcttgcggt cgttgaattc ccagaacaga
      601 ccgcctgcgg catcctgctc ctcgatggta tcaaacacgg ctttccagta tgcctcatac
      661 tcatccgcag gcagagagga atgcacattg aattcggtaa cgacgaacgg ctttccgact
      721 acttcgtgag aatctgcgat ccactggagc atggtcgtct tggtgtcctc cggtgtcata
      781 ttcgcccact gttcatccgg atacggatgc gcagagcaga agtcgatata gggtgactgc
      841 tggatgtaga cgaacgggtt gccctcgtcg ccgccgaatc cgtatttcag accgtggcct
      901 tccagaccgg tatagaccat gtgattcggg tcaacgctct tgatatactc gcccatttca
      961 tccacccatg cacggagtgt cagaccagtg ctgtcctcgc cggcatcctg gtaacggggt
     1021 tcattcatca gatcccatgc aaaaatggtg ggatcgtctt tatactgcac accagtgaaa
     1081 tagttggtac gctctgcaaa gtgcttggcg tagtccttat accactgctt gcaggtctcg
     1141 ttgctgaaat actgcgcccg gttcgcatgg ctgccgccgg tcagtcctgc ccagctcagc
     1201 tttttgtcaa tgccgccgta tgcctcccag tagttttcca gcgtgatgat aacacggatg
     1261 ttatgctctt gtgcagagta catgatatag tcgaaaagca tgaactctgc ctcgttatac
     1321 tcgcccggtg caagttcaaa tccgtgccag gtctcattgg aaaagcccca ggtacgcagt
     1381 accgttacgc cgttgtcctc catctgctgc atccggctgt cgatttgatc cttgtacata
     1441 tacttgtttt cgatatcctc gttggaatct ttgctggagc cgtcgcccaa agtgaacaga
     1501 tcgtaggagt tgcatccggc aaaatagaac ggtgcgccgt cacacatgaa gtgtgttccg
     1561 tctgcataga caaagccttc cggcacgctg gatgtttttg cagccgctgc cgtttcgctg
     1621 tctgctgccc aggcagtgga atagctgccc actacaccgc acacgcccag taccgcactg
     1681 gcgcagagtg ctgtgatctt cttcagatat ttcataagct gctcctttct cacttgctct
     1741 ccggcaagct gttgatgatg tgtaccagga accggagcag tgccagtgcg tcatttccgc
     1801 ctactgtgcc gtctgcatcg cagtccgcat tcgttctcgc agattcgttc agttccacca
     1861 cgccggcagt tgccttattc agcagcactg cgtccgcaat atctaccttg ccgtccagat
     1921 tgatgtcacc atacaggaca ctgcccggtg tttcctcgga tgttgcagta gtctgagaag
     1981 atgtctctgt ggcagatgcc gatgttactg tctccgaagt agtagcagat gctgtggtct
     2041 ccgtggtatc cgaagaaacg gtttcagaag tctttccggt atcctcagtg gatgtagaag
     2101 tttctgtctc tgtggtggta gtttccgaag ttgtggttac cgttgtctct gtagtttctg
     2161 ttgtgactga gccgtccttc acggtaatgg tcagaaccac atccgggcag tctgcaaagg
     2221 aaacgtgtag ggtgtgttcg ccagctgcca gcgtatccag ataggagcgg ttgaaccgga
     2281 actggctgcc gtttactgca aagtctgttc cctgctgcaa tgcgttgccg tcctcggtga
     2341 tgcctgtcag tgcgtggccg tttgtgataa tggtgaagat aatgtctgtc ggatcctgct
     2401 tatcatagac tgctgtggtc tctgttgcct ccgcatccgg tacagtcaca gtgccgtcat
     2461 gatacagacc gatgtcatcg aagtaccata cgccggtacc cggattgccg ttctgtccgg
     2521 tatagataga gaattcctgc acaccgctca gcgtcggtgt ttctgcggca gttccccact
     2581 gctgtacatg gaaatcggag aacgggatct tgacttctgt ccagccctca tttgctgtca
     2641 ccttctggat gctctcccag taagcgcctg cgccgtctac gaactggaac gtagtttcac
     2701 ggttgctgcc gtccgacagg atccagaaac ggatgccgtc aaatccggtc cagtctgcgc
     2761 cgcccagttt cttggttgca ccgcagtatc ccgggccgcc gtctgcaacg ctgtacgcat
     2821 atttcagtgc agccttgccg tttttcacgt tttccttatc cagtgtcagg gtaagtgtat
     2881 caccattgga attgggggta tatgctgcgg aaacagcctt gctgtcggca tagctttcaa
     2941 aatcatcgat cacgctgcgg gactgttcct ctgcggtaga atcataaacc cggatcagtg
     3001 ccgtaggctg tgcgccctct gttgtcagca cagttacctt atgattgccc agttccagct
     3061 ttttcagcac atccgctgca atggtgactg acgagctgct gacggtatag tcggttccgt
     3121 ttttcagcac ggtgtctccc agctgtacgc cggagatctc gcccagtttc atcgtcgcct
     3181 ggaaggtgac gtccttcnnn nnnnnnntct gccttcttcg gagaaatgct ggtctgtgca
     3241 ttccgtgcgc tcatccgctc tgcgtgtgcc atgatgacat cacgtgcatc attggttttc
     3301 ggataatccc ggtagtatac ggtgtagccg tcatagtcct ggtacaggct gtcgccgtcc
     3361 atggatgcca gcatccagta gtttgtgccg gcgaattcga cgccctcata ggttttgcct
     3421 tcgaagatgt cgaaccaatc tttataaaca tcggctctct cactctgatc cttccagcca
     3481 aattcttctg caatcagcgg cttgttcatg gctgcggtat cctcgccgtg tttcttgaac
     3541 cagaactcgc cctgttcttt ggtgagtccc cactggtcgc agtatacgtg cagtgtgccg
     3601 aagtcgatat ccggcaatgc tgtcagttgc aggaaatcca tgccggagct gccgtggtat
     3661 acgtacttgt ggtcaccctc cgggaagtcg ttgtagccgt agtagtaaaa gccctcatct
     3721 cccactgcca gcatatggtt gctgtcgatg gactttacgt attcgctcat ttcctttgcc
     3781 cagttgacaa ccacattctt ttcgcagccg gcgtcagact cgcaccgggg ttcgtttgcc
     3841 agctcccagg agaaaacagt gggatcgtct ttgtatttca cgccggtgta cacgttctcg
     3901 tggttgagca gcgtcttgac gtagttctta taccatccct tgatggtttc gttggtgtag
     3961 aagtcatcgt gtccggtgac attctcgcct gccagctgcg cccacttta
//
LOCUS       DAWENO010000185         4009 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_7948_length_4009_cov_9.018715, whole genome shotgun sequence.
ACCESSION   DAWENO010000185 DAWENO010000000
VERSION     DAWENO010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7948_length_4009_cov_9.018715"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    586..595
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1001 a         1031 c         1141 g          826 t
ORIGIN      
        1 cctatcaatc acagtgctat agtataatga tacttgcaag gataaatgac ggcgttcgac
       61 agcaaaagct gcggcgctgt ccgggaatcg gttctgtgtt tgttcataga tatcttattt
      121 taataggaga tatgtccgga tgcagaccga ctctgggaag aaatcagaaa accctgatgg
      181 cagtcaccgg agcggtgctg cccgattatt gaggaaggcg tgaaaagtta tggcattgga
      241 aaaagttctt gttgttgacg atgacccgaa tatttgtgac gtactgcgga tgtacctgga
      301 aaacgaaggc tacagcgtaa ttctgtctta tgacggcgaa gaggctctgg tgaagttcaa
      361 cgcactgaag ccggacatca tcctgctgga tgttatgctg cccacactgg acggctggca
      421 ggtttgccgt gaggttcgta agaagtccag cgtgccgatc atcatgatca ccgcaaaggg
      481 cgatactttt gataaggtgc tgggtttgga actgggcgca gacgactata ttgtaaagcc
      541 gttcgacacc aaggaagtca tcgcacgtat caaggcaatc gcaagnnnnn nnnnntgggc
      601 aattctccgg aagagtccga ggtcaaggaa gtacgctatg acaagctgtc tgtcaacatg
      661 actcgttatg agctgcgtgt agacggcaag gttgtagaca caccgccgaa agaactggaa
      721 ctgctgttct atctggcatc caacccgaac cgtgtttaca cccgtgacca gctgctggat
      781 gaggtatggg gctttgagta ctacggtgac tcccgtacca ttgacgtaca catcaagcgt
      841 ctgcgtgaaa agctggaagg cgtttcggac aagtggtctc tgaagactgt ctggggcgta
      901 ggctataagt tcgagtctga agaagacgaa taaatcccgt ggaaaaacca gaacaaagcc
      961 ggaaaggcaa gcattgctgg cggcgcatct ggcggaagtt cttcatctgg atgctgctgg
     1021 tcacatttct gctgatcgtg acagtgctgt cgctttcggg ctatgcgatc tcaaaatata
     1081 agcaggatta ctatggcagc tgcctccgtt cctgcgatat tatggcggaa gagttcacgg
     1141 aatacagcag acagaatccg agagaggatc tgctccggga ctatatcccg aaaaatatgg
     1201 ataagatcgc ccagcagaca gatgcttttc tctatctgtt caatcaggac ggggactgca
     1261 tctatgccag cgaaaacagc ggcatttctg cggatgaact gaaactcagc acagaaatgc
     1321 gcagcggttt gcaaaaagac ggcgagtata tgaccgacag caatgtcgga aagatcgacc
     1381 acagcgcagc agaacccatg ctcacccgtg gcagaacgtt ttatctggtg cggaacggtg
     1441 aggaagttcc ttattatatg tttgcctcgt cctacacacg gcagctcaat gtgtatgtta
     1501 cacacatgat gctgctggtg gtcatcctgt ttttcgtgat gctgctgctg tccgggcttc
     1561 tgacctttta cggcagctgg aacagcaacc gccggctgga acagctggag acagcaatgc
     1621 ggcgttatgt gcagggggac tacagccagt ccattgacac ggattccttc gtggggacgg
     1681 aactggaaca ggtggcatct ctggcagaac agatcggcag ccagtccggc agagccgaag
     1741 agtccagccg ccagtttgtg tccaacgtgt cccatgagct gcggacgccg atgaccatta
     1801 tcagcggttt tgtagagggc atcctggacg gcactgtgcc gaaacccaag cggatggagt
     1861 atctctccat tgtttcgcag gaggtacagc ggctgaaaat gctggttact tccatgctga
     1921 atctgaccaa gttcgatgcc ggcacgattc agatgaacta tcgtatgttt acgctgaacg
     1981 ataccgcatt ccggacgata ctcatgtttg aaaaccgtct ggaaaagcga aatattgcgg
     2041 tggaaggact ggacagtccg ccggtgcgtg tctgtgcaga cccggatctg atcggtcagg
     2101 tggtttataa cctggtggaa aacgccgtga agttcgtcaa tgacggcggc acgatcacgt
     2161 ttacctttgc cgacgaagaa aatgactggg tatttgccgt ccgcaatacc ggaaagggca
     2221 tttcgaagga agaactgccg aagatcttcg agcggttcta taagtcggat gcctcccgca
     2281 gccaggataa gaccggattg ggtctgggac tggatattac gaaaaagatc attcacctgc
     2341 accacgccca gatcagcgtg cgcagtgagg aaaatgccta tacggagttc gaagtgcgcc
     2401 tgccgcacat ggaaccgccg gaacaggaaa acgaataaga gaacggacag tgtcaaagtg
     2461 aaaaaatgca ggcactgtcc gtttttttga aaggagaatc tttcatgcag gagcagcagt
     2521 ctcaggcatt ccccaatgcc aatgcagaca cttccccaac accagagcag aatccgtcgc
     2581 aggaaaagcc ggtgaacccg gtctcaacgg aacagaacgt gtgcccggca cagcccggaa
     2641 atcccgtgcc gccggtgcag aatgtgcgcc cggtacagcc cgggaatccc gtaccgccgg
     2701 tgcagaacgt gcgcccggta cagcccggaa atcccgtgcc gccggtgcag aatgtgcgtc
     2761 cggtacagcc cggaaattct gtgccgccgg tgcagaacgt tcgcccggca cagcctggaa
     2821 atcccgtgcc gccggtgcag aatgtgcgcc cggtacagcc cggaaattct gtgccgcctg
     2881 tgaatcctgt gccgccgata caccaggtac ccccaatgaa ccaggttccg ccgatgcaca
     2941 accccatccc caactggtat cagaaccagc cgccgaaaat gggcagctgg caggcaccgc
     3001 cgcaggtgcc gcctgtgccg cagaaaccgc ccatgtctcc ggagaaaaag gacagacgca
     3061 cggcgttctg gctgaaaatc gtggcaggac tgctggtagt gctattgctc tactgcatcg
     3121 gcagcgacgt ggtgaaatac tgccacggcg agactgtccc ggctgcggca tcctccgaca
     3181 cagtcaacga ggatacccaa aaggtcatca tccagcagca ggataaaccg gagctggaca
     3241 aaaacgccga gaatacagac gaaaacggcg catataccgt ggaaggcgtg gcggcggcag
     3301 tgcgtccgtc cattgtggag atcgagacct acggcgatgc caagggcgaa ctgctcagcg
     3361 gcacaggctc cgggatcatt tccagcaagg acggctatat catcaccaat gcacacgtgc
     3421 tgacggacgg caagtctttc aaggtcatca tgtccgacga atcggaatat gcggcaaagg
     3481 tgatcggcaa ggacaacaag accgacctgg ctgtcatcaa gatcgacggc agtgacttcc
     3541 cggctgccac catgggcaat tccgatgagg tagtgctggg tgaggcagtc gttgccatcg
     3601 gcaatccggc agggctttcc ggctctgtga caaacggtat cgtctccgga ctgaaccgtc
     3661 agattcgctc cgatgccacc gggttcgaca tgaactgcat ccagaccaat gccgccatca
     3721 gccccggaaa ctccggcggt gcgctggtca atatgtacgg gcaggtcatt ggcatcacct
     3781 cttcgaaata tgtcagctcc agctatgagg ggctgggctt tgccattacc atcaacgagg
     3841 tgctgcccat tgtgcaggat ctcatcgaca acggttatgt caccgaccgg gtgcgtgtgg
     3901 gcattacctt tatctcgctg ctcccggaag aggtgcagca gaactttgca gatgagatcg
     3961 gcatgaaaac cattcccgac gacctgtcgg gcgtgtggat cacggagat
//
LOCUS       DAWENO010000186         3959 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8036_length_3959_cov_10.910348, whole genome shotgun sequence.
ACCESSION   DAWENO010000186 DAWENO010000000
VERSION     DAWENO010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8036_length_3959_cov_10.910348"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          813 a         1214 c          996 g          936 t
ORIGIN      
        1 ttttttcgta aaatcttttt atgacgtttt cggcatgagt ggaaacccct cagtcagtgc
       61 tacgcactgc cagctcccct ttcaggggag cctctcttgt caaacctcac aattttgtac
      121 gattttaatc atttttttga aaatcaattt ctgtagaaag cggatttcta ttttccgagg
      181 gacgcaaaaa agctgtgcgc atctgtcacg cacagctttt tccacataaa ttcacagcag
      241 ccggttcaaa gcatcacgcc gcccagcgga cggacagaca gcttgtgaca gctgcctttt
      301 ccgaagaggt catccatgta tcggcagtat gcctccgcct cttccgtcgg aacaaacgcc
      361 tgtattgttc cggcaaagcc gccgccgtgt acccggacag cacccttgtc ccccagaaac
      421 tgctggctgt accagattcc cagagggatc gcctgctctt ccggtttctg gcaggaatac
      481 aggttttgca gcagctccca ggaagaagtc ccggacgctt tcaccagggc aaagaaagcg
      541 gagaggtcac cgttttccag tgcctttgcc tctgccgtgg cacgctcgtt ttccgcaaag
      601 aaatgggctg cccggagaat cgcccggtca gaacagcttt tccgcagttt cggaatcgcc
      661 tgccggaagt cggcggcgtc cacgtcacgc agcacttccc tgccgaagta agccgcaacc
      721 tgtttcatct ccgtcggaac tgccacatag tccggcgtca ggtcggcatg gctgcccttg
      781 gtgtcagtaa tgcaaaggca atagcctgct ttcgcaaaat caaaggtgtg tttccggatc
      841 accggtttcc gggtgtcggc aaagtcgatc cagacaaagc cgcctacgga gctgaccatc
      901 tgatccatga gtccgctctg cttgccgaag tagacattct cggcaaactg tccgatcttg
      961 gcaatctcca ctgccccggc ttttccgccg ttgtcgtgct ggtcgatgat cgtgccgatc
     1021 agcacctcaa acgccgccga agaggaaaga ccgctgccgc tcagcacatc tgaggtggaa
     1081 taggcatccc agccactaag ttttgcgccc atttccgcaa attttgcggc catgccacga
     1141 atcagcgccg ttgaagtccc aacctcggca tcctttttcg acaggtcaga cagacgcacc
     1201 cgaatctccg cataaccggt ggatttcagc cggatgatct tttcgctgtg gaatcccact
     1261 acggcgatca catccagatt gactgccgcc gcaaggacac agccgtgctg gtgatctgtg
     1321 tggttgccgc ccacctcggt gcgtcccggc gccgagaaaa tgctgacctg tttccgttcg
     1381 ggatacagct ttgcaaattc ctccagtgcc tctgtatagc gttttttctg gtgcgccatc
     1441 actgcggcat cgctgccgta cagctgtttc agtttttcat ccagttttcc ggcattcaac
     1501 gcccgtttcc attctgcaac tgtcatcatt tttacatccc cttatttttt caaacttccg
     1561 aaaagtgcat ggtcaccggt gtgtaatcgc cccacgcctt cggcagcagc acaccgcctg
     1621 ccatcagcgc tgcgccggta tagacctgct ctgttccgtc cagacgatag cgcttttcag
     1681 catccagtcc acgcagctga acggtgcggc gcagaacatt tgcctctgca tggaacagca
     1741 ttccctgcac cagcacttcg ctgccgtcct gtgctgcaaa ttcccacagt gccgcatcct
     1801 ctgccatcgg atttgtcagc cgatagtact ttccgttgtg gatcagcgga ccgtaggtct
     1861 tgaactgccg gatctgctct gccacttcct gtttttctcc gtcggaaagc aaattcagat
     1921 ccagctcata gccaaagctg cctgccattg ccacagtgcc acgagtctca aacggcgtga
     1981 ttctgccggt ctggtgattc ggcacagtag acacatgaga tcccaccgca gaaaccggat
     2041 agaaaaagga tgtgccgtac tggatcttgg tgcgctcata ggcatccgta tcgtcactgc
     2101 accagatctg cggacaatag tacagcattc cggcatcgaa ccgtccgccg ccgccgctgc
     2161 atccctcaaa gagaacatcc gggaaagcgg cagtcagccg ttccagcagt gcgtacagcc
     2221 ccagcacata gcgatgcggc atttcaccct ggcggtctgc cgggagcgtc cgggtgtacc
     2281 agtcgctgat gctgcggttc atatcccact tgacatagga aatatcagcg ctgtgcagca
     2341 cgtcgctcat cacccggaac agatattcct gcacctccgg attcgccata tccagcacca
     2401 gctgataccg gctgcgcatg ggcgctctgt ccgggatgcg gattgcccag tccggatgct
     2461 tgcgatagag gtcgctgtcc tccgagatca tctccggctc gaaccagatg ccaaaccgca
     2521 ttcccatccc cttgatcttt tccaccagat catgcagacc gccggaaagc ttggtctcat
     2581 tgacaaacca gtctcccagt ccggaacagt cgtcgtcccg cttgccgaac cagccgtcat
     2641 ccagcaccag catatctacg ccaagacgtg ccgcctcttc tgcaatgtgc aggatctttt
     2701 cggtgtcaaa gccaaaatag gtcgcctccc agttgtttat cagcaccgga cgctctgcca
     2761 gctgatactt tccacggcag atatgttccc ggattgcctg gtggaagtgg tgggacaggg
     2821 tctccagtcc ggtgtcactg taggacagca tgacttccgg cgtggagaat gtctctccgg
     2881 ctttcagttc ccagcggaac agatcggggt gaatgcccat taccatacgc acctgtccca
     2941 gctggtcgca ctccagcctg gtctggtggc tgccgctgta aaccagtgca gcgccgatgc
     3001 acgctccggc tgtttccgtg cagtccggcg tgcagagcaa caccgtggga ttctggtggt
     3061 ggctggatgt gccacgggtg ctgccgatct cctggatgcc gtggaacagc aggcggcgct
     3121 ccatctggcg ttccatggcg tgtctgccgt gaaaatgcac ccactcccag ctgccccggc
     3181 tgatgtccag gcaaaggctt gccgcctttg tcagcgtcac cggtgtctca cccaggttgc
     3241 ggatcctggc gcagcgtgtc accagatcca gcgccggcag gactgcatac agcagggtca
     3301 cttcaactgc cgatgcggtg tcccggagaa ccacttccag cgtctctgcc tcgtcctctg
     3361 cggcatacac tgccggcagc ccttcgatgc catactttcc cggctttacc gtgtggctgt
     3421 aatagcgcag atccagggcg ctgctgccgt ctgcatggac cgcagccacc gccggcacac
     3481 ggaaatcgcc tacgcctgct ccggcatatt ccagcggcag cgtatccagc gaataggtgc
     3541 ggtcgtttcc tgccgcatag atgtttccgg aaaatcccac atcggggtaa tcctgcaaat
     3601 aggacatatc gcagccggtc tttgcgccgt accagagatg ccgcagcacg ccgtaggcat
     3661 ctgcctgcat ctggtacatg gtgtggtctg tctccagggt aaagatcttc ttttctgcat
     3721 caatttgaat tgccatagtc aacactccta aaatacagaa aaccgcatga catttcagca
     3781 gcattcttcg aatcgtttca tatcatacgg tctttctgtc tattctcata tttttgaaag
     3841 ggaaaggttt ttatctgtag gcaacaccgc acagagtttg tgcgtaagat gcgcagtcgg
     3901 atttttcggc agattgcaca gaaatttcgc aggcatactg tcggtcgcaa tcaatccta
//
LOCUS       DAWENO010000187         3868 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8254_length_3868_cov_9.407815, whole genome shotgun sequence.
ACCESSION   DAWENO010000187 DAWENO010000000
VERSION     DAWENO010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8254_length_3868_cov_9.407815"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3447..3456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          928 a          986 c          985 g          959 t
ORIGIN      
        1 gaactgtact ggtcatatat acttgctgcc atatatctac attataccct acttttcaaa
       61 caaatgcaat ctattttctc aaaataagcg taaattttct taacaggtga aatcaacagc
      121 agggggacac ctttttcaga tcgtgtaaag tgcaaacaca catcacgtgt ttcccaaacc
      181 caccatcact caaaaaatgc acaaacccca acagaaacag tgcaaaaaat tcggcgtaat
      241 cttttacctg ttcacgaaat aaagcttgac acatccaaat cagtccacta taataagtaa
      301 ggtattctat gtgatgtcaa acagccgccc gacggacgca ggggcggctg ttttcatgga
      361 cggttttcga gaaaggtttt ggaactgtct gaaaagaata ggaggaatga aacacatggg
      421 gagtatcaac aaacagcata tgacgattca ctggaaacac attgtcaagg acgatgacag
      481 tgtgccatca accgaggcga ccttaaaaga aaaggcgacg ctggtgggtc gtgtgggact
      541 gatgatgctg tctgtcggaa caggggcatg gcgtgtccgc aattcgatga atgaggttgc
      601 ccggacactg ggaatctcgt gtaccgcaga tatcggtctg ctgtccattg agtatacctg
      661 ctatgaggag aacggaacat atactcattc gatctcgctg cccaccacag gcgtaaatac
      721 ggataaactg acagaactgg aacagttcat gcgagagttc ccggaaaagg cagcgcattt
      781 ttccgtcaag cagattcacc tggtgctgga tgagatccag aaaaaaccgg cgaactataa
      841 ggcatggaat ctgggacttg cagcggcaat tgcctgctgt gcatttacat ttctgctggg
      901 cggcggcccc atggaaatga tctgtgcttt ctttggcgca ggcatgggca actttgtgcg
      961 taagctgatg ctgcaaaaga aaatttccct gctgggaaat gtggcagtca gcgttgccgc
     1021 atcctgtgtg acctatgtgc tgaccatcct gctggcgcag gcgatctttg gggtgtctga
     1081 agtacaccag gcaggttata tctgtgcaat gctgtttgtc attcctggtt tcccactgat
     1141 taccggcggt atcgacctgg caaagctgga cattcgttcc ggtttggagc gcatcactta
     1201 tgcactgctg attattctga ccgcaacaat gaccggatgg gtcacagcgc ttgtctgtcg
     1261 ctttcagccg gcagattttc cggcactgga aattgacacg ggcatgacga tcattctgcg
     1321 tatcatcacc agtttctttg gcgtatacgg attctcgctg atgttcaaca gcccccgaaa
     1381 gatggcaata acagccggct tgatcggaat gttctccaat acgctccggc tggaactgat
     1441 cgacctggca aatttcccgg taggcgttgc cgcatttatc ggtgcatttt tcaccggctg
     1501 tgttgcctcc gttgtcaaga aaaagatcgg ttatccgaga atttctctga ctgttccggc
     1561 aatcgttatc atggttcccg gactgttcat gtatcgtggc atttactaca tcggtttgaa
     1621 caacattgaa gatggtgcgc tctggctgac aaaggcaatc ctgattgtaa tggcacttcc
     1681 catgggactg gttgccgcaa gactctgcac cgacaagaat ttccgtcact gtacctaata
     1741 tatcagcaaa aatagaacga aaaacggaca cagctcctgg ttaagctgtg tccgtttttt
     1801 gtagagttgg tgttgtttta gagcgtgtgc agcagaaatg ctgcgtgctg ccgcagctgc
     1861 tcctgggtgt ggataaattt gtttcgttcg tgcagccgca gctggactgc caccggcagg
     1921 gaaagcagga actgccggga agagctgctt tgccgcagga gcgactgtaa attgggaaat
     1981 gccacaagaa tcacctcata tacagtatat gcagaaggga attccgatat gcgcagctgc
     2041 ggaacgcttt aagagaagaa taccgtccga acaacggcga atgccaggat agcgagtccg
     2101 gcaattaagc cgatagtatt gatctttttc gattcggcat ctttttcacc ctttaccaga
     2161 taaaagcctg cgcctccggc aatcagtgcg ccgatgagta ttccaactat gttaaaaatc
     2221 atgatttttt cctccatttc aaaagcagtg cggaattcag aatgacaaga acagaaccgg
     2281 cgttatgtac cagagcgccg gagacaggac cgagaacgcc tgtgatcgca agaataatgg
     2341 cgagaaagtt cagtcccatg gaaaaactga ggttgcactt gatggtgcgc atcatccgct
     2401 ttgacagacg gaacaggtgc ggcagttcct tgacctcatc gtcaaccaat gcaatatctg
     2461 cggcatcgat ggcgatgtca cttcccacac cgcccattgc aatgccgacg tgagccgttt
     2521 tcagcgccgg ggcatcgttg atgccgtcgc cgatcatgca gaccggctgc tgctgttcct
     2581 gggcagtgcg gatgaattgc agcttatcct ccggcagaca gccagaacgc acctgtgtga
     2641 tgcccagctg tccggcaatc gcctgtgcag cgttggcgtt gtcgccggtc aaaagaaccg
     2701 gcgtaatgtt ctgttccgat aatgcgtgaa tcatagaacg gctttccggg cggatggtat
     2761 cggacagcac cagaaatccc tgacaagact ggtcagccag ccagatcacc gtgcagccct
     2821 gttccagata acttttggca gcatctgccg cagtgtccgg gacagtgatc ttgtggtctg
     2881 tcaacagctt ttcgtttccg gcaagcaccg ttttcccgcc ttgttccacc agaacgccct
     2941 tgccgatctg catggtgaac ttgtccggct ttggaattgc cgtcccgtat tttttctgat
     3001 agcttgcagt taccgctttt cccagagggt gttccgagaa aatttttgcg gtcgctgcca
     3061 tctggaacag ttccggttca ctcaactcgc cgcagcactc cacatgaatg acctttggcg
     3121 tgccgtaggt cagcgttccc gttttgtcga atgcaatggt attcaccttg gcgagacgtt
     3181 ccagcgcatc gccctcacgc accaaaaagc cgtgcttggt ggcgttgccg atggcagcca
     3241 taatggcagt cggtgtggca agtaccagag cgcagggaca gaatacaacc agaatggtaa
     3301 ctgcacgaat cagatccttg gtgatgaatc cgatcagaat ggatgcagtc aacgctgtaa
     3361 cgacgatcca cgttgcccag cggtccgcca gccccacgat ctttgccttt ccggcatctg
     3421 cggactgcac cagacgaatc attcgcnnnn nnnnnngtct gcgccgactt tagtggcttt
     3481 catatcgaat gcgccgaact gattgacagt gccgcttgcc acatcgtctc ccggtttttt
     3541 gtccaccggc agggattcac cggtcatgac tgcctggttg acagcggttt caccggagag
     3601 aatcacgccg tctaccggaa ttgtttcgcc gggcagcacc cgaagaatgt cgccggtctg
     3661 cacttcctct gccgggatga tctcctcacc gccgttctgg atgcgtcttg ctgtctgcgg
     3721 tgtcagatgc accagctttt caatgccggc acgggctttt gccaacgtca gatcttccag
     3781 cagtgcgccc agctgcatga taaacgccac ttcgccgcag atgccttctt tctataccat
     3841 atggggtata tgtatagtat acgctatg
//
LOCUS       DAWENO010000188         3820 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8359_length_3820_cov_12.733599, whole genome shotgun sequence.
ACCESSION   DAWENO010000188 DAWENO010000000
VERSION     DAWENO010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_3820_cov_12.733599"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          863 a         1039 c         1049 g          869 t
ORIGIN      
        1 atctactata tgctctacta tgtggcactg cacggcgcag ggcagaaggt cagctgggat
       61 tacgtgattt cgtaaccggc aaaaatcaaa taccgcaaaa aagcgcatcc ggcgaaagtc
      121 agatgcgctt ttgtatgttc ggctcagaac tccttgatgg cggctttcgc cttgttcacg
      181 atgttcttgt cgttttcata ggtgaggatg gtggacgcat agccgatgtc cacccagcgg
      241 atctcggcga tctcttcctc ctgtggcacg aagtcatagt ttttggcacg agcgatgaaa
      301 tacaccaccg ttttctgcgt gtcacgtttc gggaaataag acaccgtctc ccggaatgtg
      361 gggtcgatca tgacctccac cgaggtctct tcccggatct cccggatggc agtctcgatc
      421 tctgtttcgc cgttttccat gtgtcccttg ggaaacgacc agtgaccgct gttgatgtgc
      481 tttatcagca ggatctccgt gttcccgtgg gatttgcggt agacgatagc accgcaggac
      541 ttttcatgct gcatgggttt cacccgactc ctttttcatg tgatgatttg ttacttctag
      601 tatagcatat ttttcagtat ttgtccatag ttaatttttg cgcccatgcg gaacaatttt
      661 catgatagcc tcccctgaaa ggggtgactc cccacggggt ggggagatgc ccatagggca
      721 gaggggaccg ctcagtacga gggggaccgc cgttaggcgg tggagaggtt ctttcataaa
      781 aatttttttc agtataagtg gagacccttc agtcagtgct acgcactgcc agctcccctt
      841 tcaggggagc cttacttgtc aaacctcacc attttcgccg gtacgtcaca tacacatacg
      901 gaatcccacc gccgtggcgc tcctctgcgg ttcgtgcaaa ctgtcctgcc ggcagcggcg
      961 gaaaaaacgt gtctccgtga aagtccccgg cgatctccgt gaggtacagc gtctccgcca
     1021 gcgccatgcc ctccgcataa atgcctgcgc cgccgcccag aaagacctgt tccgtttctg
     1081 cggcagcgac agcgtccgcc agggtgggaa acgtccgcag gtgcgctccg gtgaactgcc
     1141 ttgtgcggga gacgataaag ttttcccgtc cgggaagagg tacgccgatt tcttcatagg
     1201 tgcgccgccc gaagatcacc gtctgtccca gggtcagcga ccggaaacgc tgctgctctc
     1261 cgggaatgcg ccagggaatc cttccgtttt tcccgatggc acggcgccgg tcgtatgcgg
     1321 caatcatggc gatgcggctc acagggcaaa caggcacagc aggatgatga gcgacaggat
     1381 catggcaaag cccatctgga tgccgaaagc gcccaggatg aaccagccgt ttgtgccgcc
     1441 ttcctgccgc agccggatgc gccgcatttc cggggagacg tcctcctgca ccagcttgtg
     1501 tacccggcgc agcacgaaac ggtaatagat gcggtttccg aagagtccca ggagaatact
     1561 cagcacgatc tcaaagtagc tgcttatcat ggcgatgttg ttcagcaggg tctcgtgagc
     1621 gtccagcttt ccgatcatgt tctggaggaa ctggatcact tccggctggg cattcgcctc
     1681 acgcagcatg ggcagatact gctgaaacat cagttccagc tggacgatgg actggggaac
     1741 atggagcaga gacagcacgc cgatcagcac cagcgaaatg ccccacatct tccggtacgc
     1801 caggtaatac tgggggaaga gcaggcaggg aaagttcagc gaaatgcggt gtcccttact
     1861 gaaccgcagg aactttggca gatagtacag gttgttctgc gctacgaagt tcgccacatc
     1921 ttccagccgt tcgccgtcca ggtcttccgc cgggtccagt ccgcaggtcg catcctgtgc
     1981 gcccatgcgc tgtgcggttt cccggagctg ttcccgaaag gttgccggcg gcgtgtcgga
     2041 ttcctcttcc ggccgtaaaa acggcttgcc gcagtgcgga cagtgggtgg catcctttgg
     2101 acagctggtg ccgcagtccg ggcaaatgcg tatcatctgc gcctcttcct ctgctttttt
     2161 ctccggttgc cagctctttc cggattcgtg cagcggcagg ttgacgcagg cgccgttttt
     2221 ctgccagcag gcacggtgat acggcgtacc gcagtccggg cagacgacga cgtcatcgtt
     2281 gtcggtgaat acctgtccgc agacaaagca cttttctccc gtgtgtattc ccatagggta
     2341 tccctctttt ctaaggcaga atgcggagca tcgccttttt tgctccctat attataacac
     2401 aggagaaaat ttttttcaat atccaaagaa aatatttgca tcttctccga ttttgtagta
     2461 taataaaaga gatagtgatt ttgtctttcg aacaaggaga aacaattatg gtattattag
     2521 atgaactgcg tgtcacaatg agtgactacc gcaaagacct gaaagaactg tacgaggtgc
     2581 tgggcattga aaaggcacag gcacgttatg acgaactgca aaagcaggtg gcagacccgg
     2641 atttttacag cgatctggag aattcccaga aggtactcca ggaagtaaag tccctggaaa
     2701 accgcatcaa caagtataag aagatggaag agacgctgga tgacatcctc accctcattg
     2761 aactgaccat ggaagagggc gaagaagagt ccaacgccga gctgaacgaa gaggcagaca
     2821 gtttcgtcaa ggaactggaa tcgctgaaac tggcatccct gctgaccggc gaatatgaca
     2881 gctccaatgc cattctgaca ttccacgccg gcgcaggcgg cacagaggcg caggactggg
     2941 cagaaatgct ctaccggatg tacaaccgct gggcagagcg ccacaacttc aaggcagaac
     3001 tgctggacta tctggacggc gacgaggctg gcttgaagag tgcgtccatc atgatctccg
     3061 gcgacaacgc ctacggcttt ttgaagtccg agtccggcgt gcatcgtctg gtacgtgtgt
     3121 ctccgtttga ctcctccgga cgccgtcaca cctcttttgc ggcgctggaa gttatgccgg
     3181 aaatcgacga ctccatggaa gtggacatcc gtccggaaga tatcaagatg gatgtcttcc
     3241 gtgccagcgg cgccggcgga cagcacatca acaagaccag ctccgccgtt cgtctgaccc
     3301 acctgcccac cggcatcgtg gtatcctgcc agacccagcg cagccagtac cagaacaagg
     3361 actatgcgat gaagatgctc cgtgcaaagc tggtggagat caaggaacgg gaacacctgg
     3421 acaagatcgc agacatcaag ggcgtgcaga aagagatcgc ctggggcgca cagatccgtt
     3481 cctatgtatt catgccgtat accctggtca aggatcaccg taccggtttt gaaaacggca
     3541 acatccaggc agttatggac ggcgacctgg acggctttat caacgcctat ctgaaagccc
     3601 tgtccagcgg tacattgcag aagtaaggtt tgcttgtgca gagggatacc tcgaaagagt
     3661 ttcctcttaa aatgtctcct cttttagagg agatgcccga cagggcagag gagtcgtgat
     3721 ttgaacccct ccgagcggct ttgccgccca cctccccttt caggggaggc tgattcacac
     3781 caaattttct tgacaaggat tccggttttc gctataataa
//
LOCUS       DAWENO010000189         3809 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8387_length_3809_cov_11.596430, whole genome shotgun sequence.
ACCESSION   DAWENO010000189 DAWENO010000000
VERSION     DAWENO010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8387_length_3809_cov_11.596430"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          900 a         1057 c          930 g          922 t
ORIGIN      
        1 aacaaaaacc caccaattca tttccgatac ggcggcgtac ttcacgccgc taaacctccc
       61 gtcactatgg atacaatcga gcagcccgaa atgttgcact ttttcaaaaa gttttttcga
      121 tttgtaggaa tgtacaaagt tatgacttca atttgtgcat atcgtacaat ttccgttttc
      181 caaagcagat ttgaaaaaaa gcgttccatt ttccgacgat ttatgttata atagtatcag
      241 agtgcattca cacaggaatg aaacacacgc cttttcggca aaatttcaca cacagccatt
      301 tttatgtgaa tacactctga aaagcgaaac agaacggcag tgctgtgcat ccctgtgcgg
      361 cattgtctca acaaaggtgg tctttcatga acaaatacaa aaagctggtc tcgaacacgc
      421 tgatctttgc catcggttcg ttcgggtcga agatcctgtt gctgctgctg acacggcttt
      481 acaccggaaa catcaacccc ggcgacaaca gcaccaagga actgctggaa cagacagcaa
      541 actttctgat tcccattatc acattttcca ttgcggacgc tgtcatccgc tacggcatcg
      601 acaagaattt cgacaaccgg aaggtcttta cctccgcctg tgtcacagag gcatttggta
      661 tcggcgttct gctgctgctc tcacccctgc tgaactttct gccctatacc gagggctatg
      721 tggtgctgct ggtgatctac tgtatcacct ccggtttccg gcagatcgcc tcacagttcg
      781 tgcgagccag agggctggtg cggctctttg ccatggacgg cattctcgcc acgctgaccc
      841 tgtttatctt taatgttaca tttattgccg gattccatct tggcgttacc ggttttctga
      901 tctccgtcat cctgtccgac ctgctgtcgg gattgttctt gtggggcgtc gccggactgc
      961 accggttctt ccggctgcgg tatatggaca agaagatcct gcgcatgatg ctgcagttct
     1021 ccattccgct gatcccgtcc gccctgctct ggctggtgac cggattttcc gaccgtctgt
     1081 ttgtccggta tatgtccggg cctgccggtc tggtcggaga aactgctgcc ggcatctacg
     1141 gcgtttccac caagatcccg aatctgattt ccaccatctc tacgattttt ttccaggcgt
     1201 ggaatatgtc cgccatcgaa gagaacgact ccaaggaccg cagccggttc tatcatcggg
     1261 tattcgacgc ataccagtcg tttatgttta tcgccagcgc cggactcatt gtctttgtgc
     1321 ggatcctctc caacatcctt attgactcca acacgtatcc ggaatatgcc actgcattta
     1381 cgtatacgcc ggtgctgatc gtaggcgttc ttatgatgtg ctttaaccag ttcctcagca
     1441 gcgtatactc tgcgacccag cacaccaccc actcgttctg gacgagcctg gtagcagccg
     1501 tggtaaacat cgtgctgaac atcatcctca tccatatctg gggcattatg ggcgcagcca
     1561 tcgcaacctt tgccagctat ttcgtctgct actgcatccg catcgtggat gcccggaaat
     1621 atgttccctt cccggtcaac cacgccaagt ttgccgccaa caccgcagcc ctattcctgc
     1681 tgggcattgt ggtcgtggag gcgccgccga tgtggatcct cattctgacg gtaggattca
     1741 tctttatggt actgtacaac ttcagcgctg tcacccagac tctggcacgt ctgaaacgca
     1801 gataatctat aaaggcaaca ccgcacaaag cccgcctgac ggctttgcac gcaatctgct
     1861 tgctgatttt ccgtcatatt gcacagaaat cccttgacat acgacagtat gcctgcgaaa
     1921 tttctatgca atctgccgaa aaatccggct tcgcatctta cgcacaagct ttgtgcggtg
     1981 ttgcctaaaa taagtgagaa aggattgatc gctatgccaa ctccgcataa cgccgccaac
     2041 aaaggcgaca tcgcaaaaac tgtcctcatg ccgggcgatc ccctgcgtgc aaagtttatc
     2101 gcagagaatt ttctggataa tgccgtgtgt tacaacaccg tccgtggaat gttcggctat
     2161 accggcactt atcacggcgt tccggtctcc gtccagggca gcggcatggg gatgccttcc
     2221 atcggcatct attcctacga gctgtttcac gaatacgacg tggaccggat tctccgcatc
     2281 ggcactgccg gcggtgtggc agaccacgta caactgcggg atgtgattct gggacaggcc
     2341 gcctgcaccg actccaacta tgcggcacag tatgcgctgc ccggcactta tgcgcccatc
     2401 gccagctttt ctctgctgga aaaggcgtat cagaccgcaa agcgcctaga tatcccggta
     2461 caggtgggaa atctgttttc caccgatgtg ttctaccgtg agccgagtcc tctgccgcag
     2521 tgggcaaaaa tgggcgttct cgccacggaa atggaatcgg cggcactgta catgaacgct
     2581 gccgccgccg ggaaagaggc gctgtgcatc gtgaccgttt ccgactgtcc gctccggggt
     2641 gaattcacca ccgcagaaga gcggcagacc tctttcaccg atatgatgcg actcgcactg
     2701 gagaccgcct gcgcataaca gaaccgaaaa cagcctgaga atgggagaat tctcaggctg
     2761 tacttttatc aaaggagcaa gctcatgcaa acccagatca tttacgtttc cccactgggg
     2821 aacatccggc tcaccgcaga ggacgatgcg ctcatcgagg cggcatttga tgtaccggca
     2881 acgatgtcgc agtctagtac accgtttctg gaaagcgtct gtctctggct ggacgaatat
     2941 ttccagggaa aagacccggg cgcaccgccg gcgatctgtc ccaaaggcac agagttccag
     3001 ctgcgcatct ggcggctggc acagcagatc ccgttcggct atacggcgac ctatggcacg
     3061 ctggcaaaag aggcggcacg ggaaatgggc atcccgaaga tgtctgccca ggcggtgggc
     3121 ggcgcaatga aacgaaatcc cattgccatt ctgattccct gtcaccgggt agtcggggca
     3181 aatggaaagc tgggcggcta tttcggaaat gaccgccgga aaatcgacct gctgacgctg
     3241 gaagggcagg atatgcgtgg attttccaaa taagaaatgc acgcaaatac acgtaaacac
     3301 actgccaatc gtgtgataca ttgccgtacg gaataagggt aggcgaggag cttttctttt
     3361 tttgcttctt tttttctttt tagcaaagaa aaaaagaaga gttgcttttt gaatccccct
     3421 gtcagctacg ctgacatccc ccttttcaag ggagacaaga taactcctct gccctgtcgg
     3481 gcatctcctc taaaagagga gactattttt gctgcaaacg ctatgaaaat aacaaaagcc
     3541 acacccaaaa caaacattca gaaagcctgc gctgcaagta cagaaaaagg caaacaaaag
     3601 cgtttgcgca gccagaggcg gacagcgagg aacgagcgat tccgccggac aggtttgaag
     3661 agtcaagcct aggacagcgt ttttcaaaat gaaaaaggat tgacgagcga acaatgttcg
     3721 cccctacagt atgccctttc aaaatgggaa gactgactcc tctgccctgt cgggcatctc
     3781 gtactgagcg gtcccttttg tcacctacg
//
LOCUS       DAWENO010000190         3762 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8480_length_3762_cov_11.708929, whole genome shotgun sequence.
ACCESSION   DAWENO010000190 DAWENO010000000
VERSION     DAWENO010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8480_length_3762_cov_11.708929"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          849 a         1082 c          888 g          943 t
ORIGIN      
        1 tttctgactg tttcagtgca gaaaaggcgt ttaagcagcc ttattctgtg ccagatagtc
       61 ctccagattt acgtcaatgc cggcacctgc ctgtgccata tagtacatcc aaaggaacat
      121 atcctggctg tccagcttgc cgtcggcatc tgcatccaga gccttgcgct gaacgggatc
      181 cagctgtgta tcatagccgg cacctaccag agcaatattg tacatgcaga ggaacaggtc
      241 atcagtatcc acacggccgc tggcatctgc atcacccatg taatatgcag gcatggtgtg
      301 cgctgcggca gcatttatca tgctgtccag ggacgcacga agtgttttca tatcatcact
      361 tttgccggtt gccgcataag catctgcaac gacactgccg tttccgtggg aaagcatctc
      421 ggtcgactgg atcgctgcgg cagccagagc cacctgtccc accggtgtaa aggtcatatc
      481 cagattcatg atgttgctca gttcaaagcc gatcgccttt tcaccggctg cgcccacata
      541 agaggcatag aagtctgccg cagaagcatc ttccaggttt gcgatgcgct tgttcagtcc
      601 gtctgcgact gtcatcggtt ccagcacgaa attgttcaca gcgccgccct caagtgccac
      661 atccagatag gtgtataagt aagagacaac ggcacggcgg ttctgagaaa cgcccggcag
      721 tccaccatta tagtctacgc ccagagggtt gttgacagat tctaccacaa tatctgcggt
      781 acggttcagc ttgtgcagat cggtgaccac agtctcaatg ttggcaaata tggtatccac
      841 aacgcccggc agatttgccg ccagcttttt attggctgca tccagcttgt tggtatcggt
      901 ggtcagcttg tttgccacat cgatcagcgt gctgcaattg gagaggtcta ccaggtcagt
      961 gttctcatac agcacctgtg cgatcagatc gtttacgccc acagagacca tgatgacttc
     1021 tgcggattct acgtcagcac ggagcgccgc atcggactgc accgcagtca ccaggtcgcc
     1081 ggaagttgca ccgaccttcg catagtttac ggcagtgccg cccagataat ctgccacaat
     1141 ggagactgca gactttccgt cgcctgtctc tgccaggcag tcatcgccca gcgcaactac
     1201 cttacagccc gtcactgcaa ccggttcata ggtcgccgca gatgctgaca ttgccagaac
     1261 cggcactgcg gtgactgctg cgcatacgcc agccagactt tttgtccaga tttttgtttt
     1321 catcatttac aagtcctttc tgtcctgacc cgtctgcatg attcgcaggc aggtacgctt
     1381 ggcttttcca cagcggcatc ctgtctttct gcgccccagg tacccctttt tgtcccctgc
     1441 ggacagcggc gccgcctgtt gtcattatac cttgttttgc cgcatctgtc aagagatgtg
     1501 ccgggaaaaa gcgaaaccgt gtttctccaa acggaaaaac acggtttcag taaagcaatg
     1561 agaaaaagag agataactgc aaaattctaa aattgcaaaa atgaaatacc gccgacacct
     1621 cgcaattctt ttgccggaaa tgtatttcta tacgaagcat catcacaagc gggatatgct
     1681 gtgaatcatc tgccttgctt ctgttatgat tatatcacat ttgcaccaaa ttgtcaacag
     1741 gaagatgatg gatttcatgt aaaatatcca atctgctttt tgtgcaactt gacagtaggc
     1801 atccctaacg cagaaaagcg ctgtcaaaca gaccataaca gcgccgtttt ccaaaatcag
     1861 ttttccacag atttctccac ttttcaacac gctatggtgt ggaaagccgt ggaaaaaggc
     1921 agctctcacc gctttatgat ctcatccgaa tccagcatga cggtaaacgg accgtcattg
     1981 cacaactcca cctgcatatc tgcgccgaag atgccggact ccaccaccgg aacacgttcc
     2041 cggcagtacg ccttacagga ttcgtacagt gcctccgcct gcgccggcgg tgcggcatgg
     2101 acaaagctgg ggcgattgcc cttgcggcag tccgcataga gcgtaaactg cgacaccagc
     2161 agcaggctgc cgcccacatc ggaaagggac agattgatct tgtcgttctc atccgagaag
     2221 atccgcagcc gcagcagctt gtccaccatt ttttccataa tctcctgggt gtcctccggc
     2281 gcaatgccca gaagcaccag aaatccctgt tcgatctctc cggtggtttt gccctccacg
     2341 gagacctttg cgtttttcac acgctgtacc aacatcttca tgccttatcc ctcacttgaa
     2401 tttgttccag caccttgccg atgggttccc ggatgcacag gtccgcctgt tcatccatcg
     2461 gcgttgcgtc ccggttcagc agaaccagat ggtcgccccg aaaataccgc agcagccctg
     2521 ccgccggata gaccgtcagc gatgtaccac ccaccagcag caagtccgca ttggcaatag
     2581 cattcaccga ccgtgtcagc gtctcctcca gcagagactc ctcatacagt accacatccg
     2641 gcttgactct gccgccgcat ttttcacagt gcggcacgcc gtttgtcagc atgatccact
     2701 cagcatcata gacggcgccg cacacctggc agatgttccg atgcacactg ccgtgcagtt
     2761 ccagaaccgt cttgctgcca gccatctggt gcagcccgtc gatgttctgg gtcaccactg
     2821 ccagcagctt tccggcttgt tccagctccg ccagcttttt gtgcgccgca ttgggctttg
     2881 ccgtgagaca gatcatcttg ttccggtaaa accggtaaaa atcctcggta tgccgttcaa
     2941 aataagtatg gctgaggatg gtctccggcg gcacgtcata ctgctggtga tacagcccat
     3001 cctggctgcg gaagtccggg atgccgcttt ctgtggaaac gcctgcgccg ccaaaaaaga
     3061 cgatccggtc agacgcatcc acccatttct gcaactgttc gattttttct ttatccatgg
     3121 tgattcccct ttctatccgg caatgacgct gacgtaaaac gtccggcggc gtggaccgtc
     3181 aaactcgcag aagtacacgc cctgccatgt gccaagcacc agtctgccgt ttcgcaccgg
     3241 aatggtgaca ctgctgccca tggcagaggc acgggcgtgt gcggaagagt tgccctccat
     3301 gtggcgaaac tccggacgat ccggaaaggc tttccgatat gccagcagca ggtctgtcac
     3361 tacatccggg tctgcatttt cgttgatggt gatgccggcg gtggtgtggg gacagtatac
     3421 cacacaaatg ccgtctgtca cgccgctttc cgccactgca ccctgcacct gggcagtgat
     3481 ggggcagaga ttttctgccg gcgtttctaa tgtatatgcg tataacatgg caatcgctcc
     3541 ttttttgtgc tgtttctatt ataaagcatc tgtcccggga atgcaagccc ctttcttttc
     3601 aacatcaaaa acgctgattt tcttttgctt tttcaacaca agcatcaact ttttcccgga
     3661 tttttttctt tttccattga aaacagaact ttttgaaatt gtccaaaaaa atttgctgtt
     3721 ttctcttgac aaaatctgtt ttttatagta taataataaa gc
//
LOCUS       DAWENO010000191         3717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8587_length_3717_cov_10.534134, whole genome shotgun sequence.
ACCESSION   DAWENO010000191 DAWENO010000000
VERSION     DAWENO010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8587_length_3717_cov_10.534134"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3424..3433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1083 a          772 c          959 g          893 t
ORIGIN      
        1 gaacgggtgc agaatcagca gatatgattg cagcggcaag agcgcagttg ggatatactg
       61 ccccggaaac gggtgcaaag tacaaccaat ggtttgaagg aaaagaaggc acttattcct
      121 acgcatggtg tcagacgttt ctgtcatggt gtgctgacca ggcaggcatt tccacggaaa
      181 tcattgcaaa ggaatccaca gtgggaggcg agtatgcctt tttcgtaaat caaaagcgtt
      241 accgcaagag cgttgctcag ggcggaacat atacaccacg tccgggcgat attgctttct
      301 acagcgagac aagcgatgaa aacgcattga cgcacgtggg acttgtaaca gccgtaggtg
      361 aaacaagttt ccagaccatc gagggcaatg tttcaaatca agtaatggct tgtgagcgac
      421 agttggatag ttctgtggta attggttatg gatgcccgtt gtatgcagac ctgcccaacc
      481 tgtttgtacc cgaattgccg gaaatgacaa ttgagccagg ttcgacttat gtcagtacaa
      541 acttccaatg gacagaatct gccgatgcct ataattatcg tttggtgatc tggaagcgtg
      601 atacagacca gccagtcgta gatcaggaaa tgcagagcct tgctttccaa aaagttctcc
      661 cggcaggaga ttatacagca cagttgtctg cccaaaacgg agacaatatc gtttccacag
      721 aaaaacaaga ttttacagta gaagagcata acaatcccta tgattttact atttctgcta
      781 gtagcataag tgactatcgt cctataacaa tcagttggac caattctctt ggtgcgctgg
      841 ggtatcgcat ttttattcaa aatgcggata ccggagagac tttggcagaa aaagatcttg
      901 gaaatgtgca ttcgtatacg ctaaaactgg agagaggaaa ctacctggct cgtgttactg
      961 cttatcatga cagttatgaa acaactgaag aattgacatg ggatgttcgc tgtatccacc
     1021 ataatgttcc ttacctgtgg gtaatttccg caggtgatga gaatactcca actgaaatcc
     1081 gctgggattc tgcaagttgt gtaatacgat atcagctgac aattcaaaga accaaacaaa
     1141 gtggtgtccc ttccagcgat gaagatagca ttatcgtgga aaaagaaaca ggagaagaac
     1201 tggcgtggtc tggaatgcta ccggaagggt actataaggt aacgcttaga acttataatt
     1261 tggaaggaga attgtctcct tcttatgcta gctgtggaag agttcttcgc attggaaaaa
     1321 gagtaccggg agatctgaac gatgatgggg aagtaaataa aaccgatgtg tggcagttaa
     1381 accaatacat attgggagaa gcatacaaga tttccgatcc cgctactctc tctgtcgccg
     1441 atctgaatgg agatggtgtt gtgaatggct ttgatctggt gctgctgcgg cagatggtga
     1501 aataagtaga tgcactcaaa aaatacggcg aacgttctgc accaaatatg ttcaaccaat
     1561 tctccttaca caaactgcaa agttgcctat tgaaaaaagg acagcgttgt gctataataa
     1621 agtaggtgat tttccatatc tgaaaggctg ccgtatggcg gcttttcata acaatgatag
     1681 ttagcgattc ctaactatat agaaacgggg gaaagagatg aaagtttatc cctttggcag
     1741 cgaagatgct ccagcaattt tactgctgcc gggaacctgc tgccactgga agggcaattt
     1801 cggacacgtt attcctctgc tggcggcaaa ttatcacgtt ctctgcgtca gctacgatgg
     1861 ctttgatgaa acagaacaga gcgagtttcc gactatgctg gaggaaacag aaaaaataga
     1921 gacatatatc caggaaaact gcggcggaca tatccgggca gcatacggct gctcgctggg
     1981 cggctcgttc gtaggactgc ttgccgcacg gcaaaacatt caaatggact atggcattct
     2041 gggaatttct gacctggatc aggcatctgc gtttgctgca aaattgttgg tcaatctggc
     2101 aatgtcgttg atctatccga tgatacggga cggaaaaatg aaagggcgat tcctgcaaaa
     2161 gcgaatggaa aagcgggaac agcagatgcc ggaatacacg agagcttttc tgaaaatgtt
     2221 gggcggcgct cgtccttatg tcactatgca gagctgcaaa aatcagtttt attcggatat
     2281 gattacgccg ctgccggata agatcgctgt tcccggcacg gagatacata ttttctatgc
     2341 gctgaaaatg ggcgaaaaat atcgttcccg ttatcagcag cactttgcag ctcccgtgat
     2401 tcatgagcag gatttgcagc acgaagaact tctggcttgc tgtcccgaaa agtgggtaca
     2461 gctggtgaaa agtattattc actgaaaata ggaggaaaca tatgaaacac atacattttg
     2521 acattgaaaa agacggcttt tacggcgcat actggacttg tgaatccggt tcagattgtg
     2581 cagtcatcgc catgctggga gatgatccgg aagactatat ggcacgctcg gcggtgaaat
     2641 ggatcttgcg gctgggtgtg aacgtaatga ctatgtcacc tgggaaaaaa gactacggac
     2701 atcacaacta tccgttggag cgtgttgaga cagcggtaaa atggatgaaa gcacacggca
     2761 acaaaaagat cggcattgcc ggagcatcta ctaccggaac gcttgcactg acggcggcat
     2821 ctatgttcca tgacattaca ctgaccatcg ccatgactcc aagcgatttc atatggcagg
     2881 gctttatgca gggcaatcgt gacggctgta aggaatggcc gatcgaaggg gaatccctct
     2941 tttcttatgg cggaaagccg ctgccgtata tgccgttttg ctgtggacat ccgcagtatt
     3001 ggcataagat agatgcggag tccaaggaaa ccaaaagcat gatcgcatcc cgtaagctgt
     3061 tcgacgaatc cgaggcagcg catcccatta ccgaagcgga atttatcaag gtggagaaca
     3121 tttgcggcaa actgctgatg atcggggcag aagatgacgt gctgtgggat acggcaaaat
     3181 atatccgccg gatggaaaag cgccttgccg aaaaacagca tacctgtgat gtggaggcag
     3241 ctgtttatga acacggcacg cactttgttt tcccggaggg aatgctgaaa accatgctgc
     3301 ccatcggttc gggactcttc gtcaatatcg ccttcaagaa cgccaagaaa ttttcaaagg
     3361 agtgcaaaga aaccagaatt gacattgaac gccgcatgag aaatgccatc gcatcatgga
     3421 aagnnnnnnn nnnaaagtgg tataatagtc gtagaaatat ctgtatattg agcagaggag
     3481 tgcgataaca atggaataca ccaaattggg aaatacagaa attacagttt caaagatatg
     3541 cgttggctgc atgagttttg gaaaagccgg cacaatgcac gactggacgc tggatgaaac
     3601 tgccacagag tccgttgtca gacacgccct ggatctgggc atcaactttt ttgacacggc
     3661 gaatggctat tctgccggca ccagtgagga attgctggga cgtgccctgc accagag
//
LOCUS       DAWENO010000192         3630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8838_length_3630_cov_9.779580, whole genome shotgun sequence.
ACCESSION   DAWENO010000192 DAWENO010000000
VERSION     DAWENO010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8838_length_3630_cov_9.779580"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1781..1790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1032 a          761 c          958 g          869 t
ORIGIN      
        1 gcacattcac aaaaatttga aaagcgaatg ttttgaataa tttatatgca aaaatcagat
       61 gcaaaaagca aaaagaatga tagaatcatt cggttgcaat ttttcaaatc tcgtggtata
      121 atggataaga atgagaaaat caagaacagg tcggcgaggc agtggatgtg ttctggagga
      181 gttatggtat gatattattg tctgcacaga atttagaaaa aacatatatg gaacgaaagg
      241 tactggatga tgtttcgttt ttcctgggag aaggcgacaa ggtcggcatt atcggcatca
      301 acggcactgg caaatccaca ttgctgcgaa ttttagccgg tgcagaagag ccggacagcg
      361 gcacagttat tcggacaaag ggtgtgcgag tgtcttatct gccgcaaatc ccggagtttg
      421 acgcacatgg aagtgttttg gaacaagtca tggcgcactt gccgctcgac ttgaaagcgg
      481 caaaagaatt tgaggcaaag tcgattctgg gaaagcttgg cattgccgac tatgaccggg
      541 atatcagcac gctgtccggc ggtgaaagac ggcgtgtggg aattgccgct gccctgattc
      601 agccaagcga tgtactgctg ctggatgagc cgacaaacca tattgacaat gataccgtac
      661 agcttttgga agaacagctg cggaaatatc gtggcgcaat tgtgatggta acacacgacc
      721 gctatttttt gaatcgtatt acgcagaaga tcgtcgaagt ggatcgtgga aagatctacg
      781 aatataacgg cagctacagc acttatctgg aacagaaagc ccagcgtgag gcagatgcag
      841 aggcacagga acgaaagaac cgcaccctct atcggcagga actggagtgg attcgccgtg
      901 gtgttcgtgc aagaggaaca aagtcaaaag atcgcattga gcggtttcat gcgcttgaaa
      961 accgggacaa accgcaggaa atggaaaaga tgcagctgca atcggtttcg tctcgtctgg
     1021 gcaagaaaac cattgaatta gagcatttgt cgaagtcgat tgacggaaag cagctgctcc
     1081 gggacttttc ctataagctg ccccgggatg ccagaattgg tattgttgga cacaacggcg
     1141 caggaaagtc tacgctgata aagctgattt gcggtgaact tctgccggat agcggcaata
     1201 ttgtgttggg agataccgtt cgtatcggat atttttccca ggagtgcgaa aagatggatc
     1261 cgtcacagag agtgatcgat tatatcaagg agaccgcaga acgcattcag acgccggatg
     1321 gcacagttac tgcggcaact atgctggaac gatttctctt tacgccggaa ttgcaatgga
     1381 accgcattga aaagctttcc ggcggcgagc gaaagcggct gtatctgctg aaagttttga
     1441 tgactgcgcc gaatgtactt cttttggatg aaccgaccaa tgacctggat attgctacgc
     1501 tgacgattct ggaagattat ctgcaaagct ttagcggtgc tgtaattgcg atatcccacg
     1561 accgatattt cctggacaaa atggcgtctg agatctggga actcagcgga gatggctgtg
     1621 tgcagcgcta taacggaaac tacagtgatt acgcagaaaa ggtggtctct gtttccgatg
     1681 cggcggagaa accgaaaaag gctgcggaga aaaaagaacg tgtttttgtc ggcgaaaaaa
     1741 agctgaaatt ttcctataag gaacagcggg aatatgatac nnnnnnnnnn gatcgacgat
     1801 gatattgcag cgctggaaca gcagatcgag gatacagaga ctgaaatcgg aaaaagtact
     1861 tcggattatg tgaaacttca ggaattatct gatcgaaaga ccgccttaga acagcagctg
     1921 gaagagaaaa tggagcgctg gctctatttg aatgatctgg cagagcgcat tgctgcaagt
     1981 cggtaaaata tcatatagaa tgaaaaagtg ccgtttatgg aaatccaaac ggcacttttt
     2041 agtttattct gtttttgtga ttcgggcttg atatcgtttg gatgggcgat agcaaagcag
     2101 tgcaatgccg atgagaacgg cagttgttgc aagactgacc ggatacacca gcataatggt
     2161 ggcgaacgtt cggctgtgct ggaatatggt gtagatccag aagatacgga tgccacaaac
     2221 gcctaccgtt gtaagaagtg cagggacaag tgaaatgcca aatccacgga gatagccgga
     2281 catgacctca tacagcatac tgaaagtata agcggaaaag atgatgacca gacgggtgta
     2341 accgatttca atgacctgtg ggtcactgtt gaagatggac aacaggaatt taccggtgaa
     2401 taagatcaaa ccgatggcta ccgcagaggc gatggcgtct tccaccaggc agagtaccag
     2461 cgtccggcga cagcgtttca tattccctgc accatagttc tgtccgacaa aagttgtgca
     2521 ggcctggcta aaggagttca gcacatcgta tgccagaatt tcgatgttga atgcggcgct
     2581 ggatgcggca attacaacgg ttcccaggct gttgattgcg gtctggataa cgatgttggc
     2641 aacggcaaaa acggcgctct ggattcctgc aggaagtccg atctgaataa tgcggatgag
     2701 actgtgacgg tgcattttca actgccgcag ttcgacccgg acacatttat cggtgcgtat
     2761 cagcttatac agcaaaagca gagaacttac aacattggaa agtacggtgg caatcgctac
     2821 gccgtttacc gccatatgca gtaccatgac aaagaagaag ttcatgataa cattcagaac
     2881 accggaaatc aacagcgcct gcagcggcac cttggtgtcc ccgatacttc ggaaaatggc
     2941 tgcctcaaag ttatagagga aaataacggg cattcccaat aagtaaatgc gcaggtattg
     3001 cagtgcataa gggtatactt cgtcgggaat attcaggatt cccatgagct ttccggcaat
     3061 caactcgccc agcaaagtga caatgatgcc gccaagcaca gccataacaa tagaggtatg
     3121 tactgctttg tgtacacttt ccatgtcttt gcgtccgatt gcgttggcaa taacaacgtt
     3181 tgcgcctagt gctatgccaa taaacaggtt taagatcaag ccgataatgg gactgtttgc
     3241 gccgacagct gcgaccgcaa ccgttttatc tccggcagaa aaattgccca caacggcaat
     3301 gtcagaggca ttgaacaact gttccagaat ggctgtggct gccactggca gagcgtatcg
     3361 tggcagcttg ttccagatcg aaccatggag catatcaatc tttgcgtgtg atgtttcggt
     3421 ttctgtcgtg ctgttgctca tacatcgaac tcctctctta gcatttgttt tgtgattttt
     3481 ccatcccgga attgctgcat catttcattg gtcaggctgg catcatctgg ctgtaatgca
     3541 atttcttttc ggttgaccca ttcggcatat ttcagttcgt cggtgtccat acggatcgta
     3601 ctgtcaccgt caacttcaca gaaaaagccg
//
LOCUS       DAWENO010000193         3627 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8842_length_3627_cov_14.882979, whole genome shotgun sequence.
ACCESSION   DAWENO010000193 DAWENO010000000
VERSION     DAWENO010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8842_length_3627_cov_14.882979"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          868 a          994 c         1040 g          725 t
ORIGIN      
        1 gattctgaca ttcataacct ctgcgaggac ttggtacagt ataccgatat tgctcccaaa
       61 tactatgaac gcttcgatgt gaaacgaggt ctgagaaatg cagatggttc gggcgtcatt
      121 gctgggatta cccggatctg tatggtacac ggctatgtaa tgagcgaggg tgagcgccag
      181 cccatccccg gtcagctgat ctaccgtggc tatgatatca atgatctgat cgaaaacgca
      241 gagcgggaag accgctatgt ctttgaagaa gttgcttacc tgctgctgtt cggcagactc
      301 ccgaacgggg cagatctgga gcgcatgaca cgttctctcg gcgttcgccg tgagctgccg
      361 ccgggattcc tggtggactc catcatgcag tctccgtcca ataatattat gaataagctg
      421 gcgagaagca tcctgtccct ctattcctat tccgatgtga cagagaatac caccattgag
      481 gacgagatgt ccatcgccct ggatctgatc gcaaagatgc ccattctcat ggacggcgca
      541 taccaggcaa agcggcataa gtacgacaat gccagcctta tcatgcaccc gctccgcccg
      601 gaggaaaaca ccgcagaatc catcctgtct ctgattcgga acgaccgttc ctacaccaag
      661 gcagaggcac agctgctgga taccctgctg gcgctccacg cagaccacgg cggcggaaac
      721 aactccacct ttgcctgccg tgtggcgacc tcttccggca cagatccctt tgcggcatat
      781 acttccgcca tcaactccct caagggaccc cgtcacggcg gcgcaaacat caaggtcatc
      841 cagatgctgg acgacatcaa ggcgcacgtg gcacactggg aaaacgaggg tgaggtagcc
      901 gactatctca ccaagatcct gcggaaggag gcaaacgacg gctccggtct gatctacggt
      961 atgggacacg ccgtctatac cctgtccgat ccccgtgcgg tcatcctgaa gcgcaaggca
     1021 atggcgctgg ctgccggcaa ggaaatcgaa cgggagttca agctcctgga taccatcgaa
     1081 aagctgacgc cggaactgat gaagtccgtc cggggcagcg acaaggtggt atgcgccaac
     1141 gtggatatgt actccggtct ggtataccgg atgctgggca tcccggacga gctgttcaca
     1201 cccctgttcg ccaccgccag aatggtcggc tgggcggcac accggatgga agagatactc
     1261 accggaaagc ggatcatccg cccggcatac aaggcgatcg caaaatcgaa ggagtatgtt
     1321 cctctatgcg accgataaca caatcgagga tgcggcggcg gcggtggctg ccagccgcat
     1381 tttgcctact ctgctgcctg ctgttctccg gatgtacgcc gggcgcaacg gtggattccc
     1441 tgctgaaacc gcccagtctg agcgaagaac agcagcagat ctacctggcg ctccaggatg
     1501 cagtgggcag cggcattacg ctgcagtatc ccagagccgg tgtcaatctg tcggcgttca
     1561 cggtagttga cctggacggc gacggcgagg acgaggcgct ggtgttttat aaaaagacga
     1621 accttgccgc cgtggaaaac ggactgcggc tcagcgtcct ggaccaggtg aaaggaaagt
     1681 ggatgtcagt ctgcgaccgt ccggcggacg gcacggaagt ggaacgcatc gtggtctccg
     1741 ccatgggcga cgagacccag aaccagatct tcgtgggcta cagcggcgta gaccagtcgg
     1801 ataaatcgct gacggtctac cgctacggcg acagccaggt ggaacagctg ttcaccgccg
     1861 cctataccat gttcgacgtg gcagatttgg acggcgacca ggcgcaggaa ctgctggtgc
     1921 tgggaaagct taccgaaaat gcgtcctcct ctgcggcaat gtaccgcctg caggacggca
     1981 gcatcaagga caacggcaag ctggatctgc gcaccggctt cacggatttc agccaggtgc
     2041 tgtacggcac gctgtctgac ggcaccaccg gtatctatat cgacggcgcc accggacctt
     2101 cgtccttgca gacggagatc cttcacgtcc agagcgatac gctggcgtat gtgctggcgg
     2161 acggcgacac cgtggcaaag accaaccgtt cggtgggcta tctgtccatg gatctggacg
     2221 gggacggcgt ggtggagatt ccggtgcagg aaccgtttcc cggctatgca gccgatgcct
     2281 cagagcaggt gcggctcacc cggtggctca gcgtctccgg ggaacagctg accgaaaagg
     2341 aacggggcta tttcagtctg aatgacggct gtgtgttcct gctgcccctg tcctggtacg
     2401 acacggtgac ggcagtgaac gacaccctca ccggggacat cgtgttctgc cgctatgacg
     2461 gcgagatcaa cgaccatatg acagagctgc tgcgctacag cgtggcacag gataccgaat
     2521 cccaggaaga acgggagacc gaagggtatc gtctgctcca cacccgtggc aaggcatcgt
     2581 attatatgaa accggcagag acagacgaca gcctggcaca gccctggcag gaactgatgg
     2641 tgcggttttc ctttgtgcag tgaggatttt ttctgtgtcg gagggataaa tttaacagag
     2701 gaaccctata tcggaaatgg aaaaccatac ggcaacgtat acgttatcct ctcaaccgta
     2761 ggggcgaaca ttgttcgccc gaaactcctc agtcaagcct acggcttgcc agctcctcta
     2821 taagaggagc caagagaaaa gtctcctctt ccagaggaga tgcccgacag ggcagaggag
     2881 tcgattcgcc ctgtatgaaa ggaaatatcg caggaggcgt gacgggagaa cgatgttcgc
     2941 ccctacagaa gaaaccttat cccctgttct tttatagtag aagggataag ccttccataa
     3001 gttaatatgt cccccttgaa aagggggatg tcccgaaggg acagggggat aatctgcccc
     3061 ttcaaaaacc aactctatcc gtctttcaga atgtgaatta agataacaaa cagtctctca
     3121 aacagaggtg taagaatgaa acgtatttta gtgtgtgaag atgaagatgt catccgggac
     3181 tttgtcgtca ttaacctcaa gcgtgccggc tatgacgtgt gcgatgtggg atgcggcgag
     3241 gacgcactcc gtgtgtttga agaggaacac ggaaatttcg acatcgtgct gctggatatc
     3301 atgatgcccg gcatcgacgg ctttaccgtc tgcaaaaagc tccgggaaaa aagctctacc
     3361 attggcatta tcatgctgac ggcgaggacc caggaaatgg acaaggtcag cggactgatg
     3421 ttcggcgcag acgactatat cacaaagccc ttttcgccct ctgagctgac cgccagagtg
     3481 gacgccatct atcgccgtgt ctgcatgagt tttatcaagg atgaacagtc gcccgttatc
     3541 gaaagcggcc cgttcaccct caatctcaag agccggaccg tgacgaaaaa cggcaagctc
     3601 attgatctga cccaggtgga atatcag
//
LOCUS       DAWENO010000194         3605 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8908_length_3605_cov_9.621972, whole genome shotgun sequence.
ACCESSION   DAWENO010000194 DAWENO010000000
VERSION     DAWENO010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_3605_cov_9.621972"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          820 a          988 c          899 g          898 t
ORIGIN      
        1 tccgaacgta gccggtgtta tcggttctgc aatcgcagcc ggtttcctgc tggcagtatt
       61 taagtaaact tcctcgtttg catatcattt cccgttccgt atcctttcgc acaacagata
      121 acggaacagg ttctaaaaac aaaacgcccg atacaagcaa tatacggctt gtatcgggcg
      181 tttttgtgtt agcaggagaa gagacaccgg aaatcatggg aaatcgctca cagcaggctt
      241 tccgtggatt ctgagcgata tgcagtctgg cagttccaca tcgctgtgca ggaaagcttt
      301 gcattggctc tgtccggcgc agcgatttac cataacaaac aaaaatcccc gcaggcaaac
      361 acgctgcgag gaacaatgca aatattcatt gttgtgtcaa aatcaagatg tatggtcact
      421 tccgaaccgg aagctcgtca cccctgtggt gacggcgaat caaaagaaaa gataaattgt
      481 agatcgaaat ttcagaatca aaaactaata gatgagttta taaagattgg ggaacaatgc
      541 caggtattcg ctcagctttg tacgcttgct ggaacccgga tcgttgatga taaagttttc
      601 tgcggagaac gcagtgcctt ttgaaccagt gtaaccggta atcagcacgt aatgctggct
      661 gccgttggac ttctttgcac cgatcacaac cggtgtgttg ttcagcagct gatcgtagat
      721 cttctgcatc accttctgtg aaatgctgcc actgacatcc tctacctggt atcccagctt
      781 ggtgcaggac gaccagatca gattgtcact gctgtaggtc agcttggaca ccatcttatc
      841 cggtgtagta ttcgtgccgg tctggtaaga atacttcatc gccagcgctg tggtcacaca
      901 gcctaccttt ccgatggtag aatagttgat aaccttgttg ctccacttgg agtcgaactg
      961 tttatagtcc accacgttca gctgtactgc ctggtgacca gtctctgtca tgatgccggt
     1021 cgtcggatcg aagtaacggg aagtgtcccc tacggtcacc cagccagtct gcatgaaaaa
     1081 tgtggacggg ttaaagtaat aaacgtctcc gttaatggta atgagtccgg atgctgcgcc
     1141 gttctctgtc atatagagct tgccgccgtc ctttgcagtc cagccggtat atgccgcgcc
     1201 gctctccgaa ttgaagtaat acagttttcc gccgatctcc tgccaatctg taagacgaac
     1261 gcctgtcgca gcgtcaaacc cgtaaacctt tccgtcaatg gtctgtacgc cagttgccac
     1321 gccgtgggtg gtcagataca gcatgccatc ctctgtctcc agccatcctg tgtgcagggc
     1381 actggtagaa gggtcaaaat agaaccgtgc gccggcgatt tccacccaac cggtctgaag
     1441 tgcgccggtc tcttcgttga agtaataata gctaccgttg atctcctgaa gtccggtctg
     1501 cattacgccg ttggcgtccg cataataggt cacgccgtca atggcgcata tgccggtcat
     1561 cagataaccc ttttcactaa agcaataggt ctttccgtcg atctgtgtca gtccggcagc
     1621 gagaacgccg ctagcatctg cccacatggc agaagagccg tctttgatcc agccggtctg
     1681 catcagcttg tctgcggaga agtagtagtc cttgccgtta atggtataac gtccggaaac
     1741 tgcacccttg tccgctgtga ggtaataggt tccggcatct gtcactgccc agccggcagc
     1801 cacggtagca tccttgttgt agcagtacag cgagccgtca tcatacgtga tccagccggt
     1861 ctgtacgcat ccgtattcat cgaaggtata cggtgtgccg ttgatgctct gcacgccggt
     1921 cagctttccg gcatcctggt caatgtagta cagggaatcc tggtaattca tccagccgaa
     1981 cactggtgtg ccgacctctg cgtcataata ataacgcttt ccctcgaccg tctgccagcc
     2041 tgtccgcagt gcgccgtccg aggcgaacaa ataagtatat ccgtcgtcca gcgtggtctc
     2101 ttcctgggca cagctgccgt cgttgtaata ataataaagg ctgtcgtcat ccgaaacata
     2161 ccagcctgtc tgtgtgatct ggtctgcgtc acagctgccc gtcaatacac ggttgccgct
     2221 gtctgtcaca ataaaacggt tcaggtgcag tgtcgtttcc tgcacatccg tctcctctgc
     2281 cgaagcagaa actgcaccga tcaaaagcga tgcgagcagt ccgctggctg cggcaaggag
     2341 tattttgcgc atcggttgcg ccattggttt cacgtccttt cgtgtcaaag gaacacacgc
     2401 ccgcccgggc agcattcctt tctagcgacc gctgtttcca ttttgcaatc gctgtcgtct
     2461 gcgttcgtat ccaattgaca aaaattcgcc gtcaattgta atcgatttgt catgaatcca
     2521 ctatttatta taccgatgtt tgatgctgct gtcaatcaat ttgccgcaca gattgaaaaa
     2581 agtgtcgaat tttcggcgca ctgcacaaaa agcagatgac acgctcgccc attctgcaca
     2641 atttttcaag ctaatgaaac ccattatctg gaatattcaa aatgaaatat tggtaacaaa
     2701 acagttattt cggcaaaact atcgctgccg gactgctgat cgcaccgtaa tattccggcc
     2761 gtctgtcccg gaatacgccc cagctcatac gcatttctgc catttcatcc aggtcatagg
     2821 tctgcatgac aatgcctgct tcgtcccgtc ccagctgctg catcacagcg ccggtgcagt
     2881 ccgtcaaaaa cgatgtgccg tagaacgaca gtgccgagga ctgctgctgg ttctcttcgt
     2941 ccggcgtgat cgtctctgtg ccgatgcggt ttgccgcaat gaccggcatc agatttgccg
     3001 cagcgtgtcc ctgcatacag cgctgccagt ggggcgcact gtccacctcc aggatcggct
     3061 cagagccgat ggcagtggga tagaacagca gttctgcgcc ggcaagcgcc atacagcgtg
     3121 ccgcctccgg aaaccactga tcccagcaga tgcccacgcc gatcttggca taccgggtgt
     3181 tccacacctt aaatccggta tcccccggcg tgaaatagaa cttttcctgg tagaagtggt
     3241 cgtccgggat atgggtcttg cggtagatgc ccaggacggt gccgtccgca tcaataatgg
     3301 caacggaatt gtacagcaca ttgcctgcct tttcataaaa gctgaccggc agcaccacgt
     3361 ttaattccgc agccacccga cgcagctgct gaatcgccgg attttctgcc ggcgtggtcg
     3421 ccaggtcata ggacgcatag cggcgttcct ggcagaaata agggttttcg aacagttcgg
     3481 gcagcagaat gatctgtgcg ccgtctgccg ccgcccggcg caccatagtt tcagcggtgc
     3541 ggcggttttc tgcggcatcg gggacgcacg ccatctgtac agcggcaaca gttactttac
     3601 gcatg
//
LOCUS       DAWENO010000195         3595 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_8936_length_3595_cov_8.196045, whole genome shotgun sequence.
ACCESSION   DAWENO010000195 DAWENO010000000
VERSION     DAWENO010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8936_length_3595_cov_8.196045"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          907 a          864 c          877 g          947 t
ORIGIN      
        1 ctattgccag cggcagggcg tccaccactg catcggactg catcaatccg gagaatacct
       61 gcatctggtt gtcgaaatcg tgccgcagtt cagcaacttg ctgggatgcc tgttccgcct
      121 gggtctgata ctgcttggac agcgtttcgt attcttcgaa gaatcgtatc ttttgttctt
      181 cctgttcctt ttggtacatc tttctcatga agtacagcag cacaatgctg gatactacat
      241 ttaataagat ggttgccccc agtaacagct gcattcccgg agatgtgccg atggcatgga
      301 actgctgcaa aatggtgacc agtgtgtaaa tggcgaagaa ctgcgccagg ggaaatgtca
      361 tcagacagag cacctgtttg tggtttttca gctggatatg cagacgggga tagagccatt
      421 tcagcagggg aattactgcc aggtacagca gtactaccgg catggtagtg aaaagattat
      481 accacttcga ccagcgctct gtggatgcaa taacctcggc atagtgtatc ccgaacactg
      541 catctacagc gccggcaagc aaaacaacag aaatatctgc tgcggcgaaa atgatgaaat
      601 ttaccactgc attaaaacag cgtatcagaa tggattcctt cggatacgct gtcagataaa
      661 agacgaagaa tatgctccag tgcaggagcg tgtaaagttg ttcgtgtttg tgcaggatcc
      721 agtaggacgc atcgccgtac agcatccacg gaatcagaca gaacgtcaaa ctggttgcaa
      781 atgttataat gccgttccag actgttgccg caattttcgc ttttcccatg gtaacacggg
      841 tgaatagcag cataaacagg taaatgagca ttacagacac ggtgaagttg aaggtgtaga
      901 aaatgtttgc catccgctcc acctcttaac cgtgcagttc gcttttgcgg cggatcagtt
      961 cgtggcggtt gtctgcaaca gaacgatgtc caaaggcgct gatgggaatg ctttcgccgt
     1021 atattgtgac gcttttgttt tcgatgcact gtacatgatt cagattgatg agatagcttt
     1081 tgtagcactg gtaaaaataa tgcggcagct gttttaccag gtcagaaagc cgatagtatc
     1141 cggtgtagtc tccacgggcg gtgtgcgccg tgacatagcg tctgtcgctg gaaaaataca
     1201 gcacatccgg cagggaaaga tagaccgcct gtccgcccac cggcttaaag gcaaacactt
     1261 ccgatgaatc tgaagtatcc gcctgcaatt tcctgacata gttcagcaca tcgtctttct
     1321 gaaaaggctt tggcagataa ccgtatggtt tcacgtggct gctcagaaag atctgctgca
     1381 aatagtcctc cggatgcccg gtgacgtaaa taaactttgt ctggctgttg gattttgcca
     1441 gcttttccgc atagtcgatg ccgttttgcg tgctgccctg aaagcagata tccataaaga
     1501 cagcatccac atgggcgtgc tgtgaaaaat aaaactgcag cgtgcgccat tcctggaaat
     1561 attccacggt catatcactg ccgctttcct ccagcagctt tttcagctgc agtccgatgc
     1621 acggctcgtc gtcaaagatt acgattcgca ttaattctcc atccctctta tatcgtgccg
     1681 cattgcacga tctcatgatt cggcggtgtc cgcttgactt gtattcctaa gaaatttttg
     1741 catatgctta tgaatacaaa tccttattca ttataacata aatcattcgg taaaaatagg
     1801 ctttttctgg aacaaaaata tttcagaatt atggcataca gaccgccttt caccatcttg
     1861 ttttgacaag aaacagagca cttatgacaa aagccttgtc ataacggcaa ggcagtgcta
     1921 taatagcatc gtagaaaagg agcgtgcggc gtacaagata cccagttcct tttcgaactc
     1981 ctttcgcatc acctgccgca gcggcaggtc acaaaaaact ttccatccct ctttgaaagc
     2041 agtgaaaatg caaagggaaa agcctgttta gtatgggaaa gcgcattcat ccgatacaaa
     2101 tatgtgctgt aaactttggg aaaatgaaaa aatgagacac acggaaaagc ctgccgggca
     2161 tcgggaatgt acgacaggct ttcgtgtatg ccgaaacgaa aaaagagacc gcagtctttc
     2221 tgcggtctct tttgtattgc cagaaaggat tactgaattt ccttgacata gttcaccaga
     2281 ttgccgatgc acaattcaaa ttccacactg tagcacggca gcttttcccg gtgtacgtcc
     2341 tcataatccg cctgtgtgac ctcgatggtg tcagcggtga agttgaccgc aatggacagc
     2401 gcctgcgcaa tagacgcacc acgcaccagc gcaccggaca gaacgcttgc gaaaatatcg
     2461 cccgtgccgt gcatccgttc cggggtgtgt ttccggtagg attcgtagaa ctttcccgtt
     2521 tcgctgtcat aggcggcagc gccgtgaccg tcacccttgc ggacaccggt gatgaccgcc
     2581 gtgcggcatc ccagttcggt cagctgtttc agatagctgt ggatctgctc catgtcacac
     2641 gtttccgcat aaggcacatc caaaagcaga gaaacttctg tcagattcgg tgcaatcacg
     2701 tctgcttttg cgcaaagctt tcgcatttcc cgggcaaagt ccggcgtgaa tccggcatac
     2761 atttttccgc cgtctcccat gaccggatcc accacgatgg tggtgtcctt tttgcggaac
     2821 agatcgaaaa agtctgcaac gatctgcacc tgctctctgg aacccagata gccggtatag
     2881 attccgtcaa agtgcagatc taagcctttc cagtgcgcag cgatctgggg gatatctgct
     2941 gtcagatccc ggaaggtata accggtgaat ccgctggtgt gggtggaaag aacggcagtg
     3001 gggatcacag ccgcctctac gcccattgcc gagatcagcg gcagtgcaac ggtggaagag
     3061 cattttccga aacaggagat atcctggatg gtcacgattc gtttcagcat gaaagggtca
     3121 cgtccttatg tctcagaagt ggacttctga aaagattttt gtacatgatt tttgatggct
     3181 gcaaagcttt ctgcactgat gacgtgttcc atgcggcagg catcctccac agcgatttcg
     3241 ggatcaacac cgagtgccat cagccagccg gaaagcaggg tgtggcgttc atagatcgtc
     3301 tctgcgatct tgcggccagg ttctgtgagc gagatctcgt tgttttcatt gacagtgata
     3361 taaccgtttt cccgcagatt tttcattgcc acactgacgc tgggctttga gaatgcaagc
     3421 tcgttggcaa tgtcaatggc gtgtacagtg ccacggcgat tgtataagat caagatagtt
     3481 tctaagtaat cttccgcaga tttttgaatt ttcaccttat ttccctccgt tatgaatttg
     3541 tgagatgctt ttctatatca tatgtgtttt ttgcggtgtt ttatccatta taaca
//
LOCUS       DAWENO010000196         3518 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9154_length_3518_cov_10.936183, whole genome shotgun sequence.
ACCESSION   DAWENO010000196 DAWENO010000000
VERSION     DAWENO010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9154_length_3518_cov_10.936183"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    576..605
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2635..2644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a          831 c         1030 g          743 t
ORIGIN      
        1 acctacggtg acatttcccc accccgtggg gaatcaccct tttcaaggga ggcattcacc
       61 atgtccccct tgaaaagggg gatgtcccga aggggcaggg ggattatatt tcaaggaacc
      121 ttctaaaaat ttttctgtgc gttttgtgca aaaaagcagt tgacaagctc cggcatttgt
      181 gctatactag ttcttacgaa agcgttgaag ggaacggcac atttcgccgg ggcttttttg
      241 cagagaaccg ctgtcaccgg ctgagagcag cggcaaaaag ccaagaaatg cgcataccac
      301 tcccggagtc tgcccaatcc gcagcggctt gtccccgtta tcggacacca cgaggtgtat
      361 gtgcctttga cacgtacatg aaacaagggt ggaaccacga tagatttttc atcgtccctt
      421 gcacagtcct ttactgtgta agggactttt tgcatttact cattctggaa aggaagatgg
      481 aagtatgatt caactgacat tgaaagacgg cagcatccgg gagctggaag cacccaccgc
      541 tgcggcagaa gttattaagg gcatcggcat gggacnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnggcag cctgctgtgt caagctggac ggcgaggtct gtgacctgcg caccgtcatc
      661 gaccatgact gcacatttga ggtcatgacc ttcgacagcg aagagggcaa aaagacattc
      721 tggcacaccg catctcacgt gctggcgcag gcagtcaagc atctgtatcc ggaggcaaag
      781 ctggcaatcg gtccggcgat cgacaccggt ttctattatg attttgacgt agagcatccg
      841 ttcagccagg aggaactgac caagatcgag gctgagatga agaagatcgt caaggcaaac
      901 gaaaagctgg aacagttcta tctgtcgccg gaagaggctg tggcaaagct gaaagagatg
      961 gatgagccgt acaaagtgga actgtgcgag gaacacgccg gaaacggtga gccgatctcc
     1021 ttctatcagc agggcgattt caccgatctg tgcgccggtc cgcacctgat ggctaccggc
     1081 acgatcaagg cgttcaagct gctgtcctgc accggtgcat actggagagg ttcagaaaag
     1141 aacaagatgc tgtcccgtgt gtatgctgtg gcattcccca aggcgtcgat gctggaagag
     1201 catctggcaa agctggaaga ggcaagaaag cgtgaccaca acaagctggg ccgggaactg
     1261 gaatacttca ccaccgttga cgttatcggt cagggtctgc cggtgctgct gcccaagggc
     1321 gcaagagtgg ttcagctgct ccagcgctgg gtagaagacg tggaacaggc acacggctgc
     1381 ctgctgacca agacaccgct gctggcaaaa cgggaactgt acaagatctc cggtcactgg
     1441 gatcactatc tggacggtat gttcgtgctg ggcgatcccc acgatgagac caaggaatgc
     1501 tttgccctgc gtcctatgac ctgcccgttc cagtatcagg tatatctgaa ccgtcagcgt
     1561 tcttaccgtg atctgccgat gcgtctgaca gagacttcta ccctgttccg caacgaggat
     1621 tccggcgaaa tgcacggtct gatccgtgtc cgccagttca ccatttctga gggtcactac
     1681 atcctgcgtc cggatcagct ggaagaggag ttcaagaact gcttgcagct ggcaaagtat
     1741 ttcctggata ccgtaggact gctggacgac tgcaccttcc ggttctccca gtgggatccg
     1801 gcaaatccgg gcaacaagta cgagggtaca tcggagcagt gggaagaggc acagcgtgtg
     1861 atgggcaata tcctggatca cctgggcgtg aagtacgagg ttggcatcga cgaggcggcg
     1921 ttctatggcc cgaagctgga catccagtac aagaatgtat tcggcaagga agataccctg
     1981 gttaccatcc agatcgatat gctgctggca gagcggtttg gtatgtacta caaggacaag
     2041 gacggtcaga aaaagctgcc gtacatcatc cacagaacat ccctgggctg ctatgagcgt
     2101 acgctggcat acatgatcga acacttcggc ggcgcaatgc cgctgtggat cgcaccggaa
     2161 caggttcgtc tggtacccat cgcagaccgt catctggact ttgcgtatga ggtcaagaag
     2221 gaactggagg ctgccggtct ccgtgttgag gtggacagcc gtgccgaaaa ggtgggctgg
     2281 aagatccgcc aggcaaccat ggaaaaggtg ccgtatatgc tgaccatcgg cgacaaggaa
     2341 tgcgaggaaa agaccgtcgc agtccgcaag cgcaacggcg aggatctggg cagcatgagc
     2401 gtggcagagc tgacggcaaa gctcaccgaa gaggttcgca ccaagcagaa gtaattgcag
     2461 aagaaaccgg acggcgtgtc cggtatcgcc tgaaacagag aaaagcgggt actgcggtat
     2521 ccgctttttt gtgcagtttt gtgaaaaatc aacattttcc agttgacaag gtcggtggga
     2581 tacgatataa taagaatagt cgtatttttt gggtgaaaga gagaatgtag agaannnnnn
     2641 nnnnaaatcc ggagggcgtg acagaatatc ccatcctgga atttgatgat cgtgcgctgc
     2701 ggctgctttc ttccggcgtg gcggagacgc tgcgctacag cgaagtcaag ccccgtggcg
     2761 gtttgggtta tcgctttgtg aaacggctgt ttgacattgt ggtttcgtcg ctgggactga
     2821 tcctgctggc aattccgatg ggcgttgtgg cgctggtgat ctatctggac gacaagggtt
     2881 cgccgatctt ttcgcaggca cgccttacgg aaaacggaaa ggtctttcgg atgcacaagt
     2941 tccgctctat gtgcgtggat gcggagaaga agttcgcaga ggtgcagaag gagaacgaga
     3001 ccgacgggct ggcgttcaag agcgagaacg atccacgcat caccaagatc gggcggttca
     3061 tccgcaaggc gtccatcgac gagctgccgc agctgtggga tgttttctgc ggtcatatgt
     3121 cgatcatcgg tccccggccg ccgcttccga gagaggttgt cctctatacg ccgcaccaga
     3181 tgcagcggct gatggtaaaa ggcggtctgt cctgcatttg ccaggtagag ggcagaagtg
     3241 acatggaatt cgacaagtgg gtggaatccg acatcgaata cataaagaca cgcagcgccg
     3301 gactggatct gaaactgctg ttcaagacca tcggcgtggt gattatgaaa aaaggcgcaa
     3361 gataagagaa taaggttctg gagagccgct gggcagggag tatgttgttg tggaaggaca
     3421 attatccttt tttgaaacga aaagtcaagt aaaaaaatat agtaaaactg ttagtgtaga
     3481 tcgtattcct gtaggaaaaa aattaaaagt catttcgc
//
LOCUS       DAWENO010000197         3503 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9198_length_3503_cov_11.416473, whole genome shotgun sequence.
ACCESSION   DAWENO010000197 DAWENO010000000
VERSION     DAWENO010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9198_length_3503_cov_11.416473"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3111..3120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          733 a          866 c          913 g          981 t
ORIGIN      
        1 caaattcgct ttgccgtctg cattgatgtc accggaaatt gcaggcggtt cttcggtgct
       61 agctgtagta gtaccggata ctgttgttga cgaaacagaa gttgctgagg ttatctttgt
      121 agttgttgta gtagtctttg cagtagttgt agttgccttt gtggtagtgg tcgttgtagt
      181 tgtcgtggta aaggcgaccg cgttccactt ctggcaggca ttgccgttca ctgtccattg
      241 ctgcacattg gcaccgctgg acttttctgc gtttgccact tctacggcag cctgatcttt
      301 tgaagtgcgt gtcaggatcg tgtatgttcc gtcggggttc ttgacaaact tgaacagatg
      361 cgaactggat ttgttattgg tgcggatctc gatattggag ccgtctgctt ctccgacaga
      421 attcagatag taggtcttac cgtccgccag agcggagaga atgtagtagt atccgccgcc
      481 gaattctttc agtgtccatg agttgtgggc tgcgggctgg tctgcacccc actgctgcac
      541 gttgctgccg tcttcagctt ttccgtttac cacttccata tacagattgc tgttggcgtt
      601 gcggaactgg tatgtcttag tggtatccat gacacagccg gtgtcctcta cctgtgtcag
      661 cttccactgc tgggaagtgt cggaaaggtt gactgcccat tccaccacgt tggctccggc
      721 acttttggaa gctccctgta cggcaacgca gctctggtca tcgctggtct tggtgagcag
      781 ataatagaca ccgtctgcgg cttcgtagaa tttgaaccgc tgtgcatcgg catcggtggc
      841 agaccagatg ccgagattgg tgccatttgc cgcagcattt ccggcaacgt ccagcaggta
      901 ggtcaccttg tcaccgatct gcggatacag cgtatagtag ccgtcaccgt ccgacagtac
      961 ccgccatgtg ttgtgagctg cgggttcatt tgcaccccac tgctggacgt tggtgccgtc
     1021 cgcagccttt ccgccgtcca cttccagata caatccgctg ttgacgttct gtatcatgta
     1081 gactgcaccc tcctgcaaga ctgccggttt gtagtcggtc accagattgc cataggcgga
     1141 atcaaagctg tctgtcaaaa acggtgctat ataaacagtt gccgcaaagg tctgcatgct
     1201 gctgtctgtg atgtatttca gttcgccgga agatgatgca gcaccggcgt atttggtgca
     1261 gaaatccttg gtatctgtgc ccttggtgtt gtaggcatct aacagtgtgt agccgtagtt
     1321 gtccttggga ttccatgtgc tggtgacttt tgcggtataa ttcagcttgg tcggggtttc
     1381 cgaactggtt ttcgccgtgt aggaaccgct gtcccgatac ggagaagtgt ccggctgtac
     1441 aatgatttcc cgtccggaaa ccgcctggaa gcggcagttt tcgctgacca tattggaacc
     1501 ctcgccgctg atgatctggt tgcagctgtt cggcaaaaag ctgtcgtcgc cggaatagta
     1561 gttgttgtag atgtgaccgc tgccgtttcc gatctgcggg cagcgacgga cgcattcgtc
     1621 ccaccaattg tacatcagcg tggtgcggca acgggacgtt tcactgttct gtgtgccgta
     1681 tgccaccgtc cagaaacgat tcttgaatgt gttataggaa atcgtgatat agtccggact
     1741 tctgccgttg tccttcgcat ccagatagct ttcatagggt gtgttcagcc agatgaattt
     1801 tcccacttcg tcctgcccat ttcccgtatg gtccgaaggc agataagaga taaacgtaca
     1861 gtgatccacc cagatctgac gcgaggacca gatattgatc aagatccggt tcttcacatt
     1921 ctttgcaatc atgttcagat tccggaaaat gatgttgtcg ctgggattgt cgtccaccgc
     1981 accataggta tcattggtac ggaactgaga atcgtaaatg gtcttgttcc cgtaagagcc
     2041 tacaatggtc ttgttgtccc gaatgcgggt atgggacttg ttctgcatat caatgtccgc
     2101 cgtgatgaca atggttttcg gcgctgtggt attcagttgc ttttccaagt cgtccgcggt
     2161 ggaaacgaaa acggtttcgc ctagcagacc gccaatagtt cctgcttttt ccttgccgtt
     2221 tgccagtgca tttgcggcgt atccctccag tccgctgcaa ttcagcttcc acttctgggc
     2281 accgtcaccg gtgtatgcgg tcagtccgat cgtattattt ccctgataat aggtcagcgc
     2341 cgcaccggtg gaaacgctgg tgatcttgta gtacagatcg tatccctcga agtctttctg
     2401 tacgctttcg atgttccagt tctgactgtt gtcagcagcc tttgccgcca gtgtacacgc
     2461 tgtgccgttg gcggtcagat acttgccgtc agccagactg acaatctggt acacgccggc
     2521 agaaacatag tcaatacgcc agtcctctga atcggctccg gcgactttct gcgtgttggc
     2581 agcgctgccg gcattgttca gattccggtt tgtggtgtac gccccgaaat gcaccgcctg
     2641 tgccggataa gccgtcacgt ccgcagccgc cacagacctt gccggcagaa tcgcaagcag
     2701 actgcccaaa attgccagta cagtcagtat ggcgggcagc aggtgtttgt tctttctctg
     2761 ttgtttcatt cccaattcct cctgtttgca tattgtggat tgtagctata ttttatcaca
     2821 atccacgagg aaattcaatc agatatgatg ctatttcata agaaatcgtg ctattatttc
     2881 tatataaaga gaataatatc ttataatttt tgttgacagc tgccgctttt ttcgttataa
     2941 taaaagcaat atcacacaaa ctttgtgcag cattgccgaa gaacagattt tcataagaaa
     3001 aggcggtgaa atcatgcagg cgttcccgaa aaagcgcggc attctggcag tgttcctgtc
     3061 agacctgcaa tcggagtaca accacacatt attagagggc atcaccaaac nnnnnnnnnn
     3121 aggagaacat tctctcactg tttcacaagg ggtatgccga cggcattctc acccaaaagg
     3181 gcatgtttca aaagccggag gtgcggcagc gaatcgacca gatctgtctg gaatccggtt
     3241 tgccgtttct ggatattgga gaggaaaacc gtgactcggt gtatccgctt tggaatgacc
     3301 ggaagatttt ctgcgatctg gtgaaccatt tgatcgaagt gcatcacaag cataaaattt
     3361 actgcctgac tggtccgccg gaacagcacc agtcccgtaa ccgtgtcctg ggatttcggg
     3421 atgcaatgca gcagcacgga cttccggtgt cggaacagat gatattttac ggagatttct
     3481 gggtgcactc cggcgaacag ttg
//
LOCUS       DAWENO010000198         3484 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9264_length_3484_cov_8.237387, whole genome shotgun sequence.
ACCESSION   DAWENO010000198 DAWENO010000000
VERSION     DAWENO010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9264_length_3484_cov_8.237387"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          998 a          755 c          750 g          981 t
ORIGIN      
        1 ttaccagaaa tcatgtttgg attcaatcta agaaacttat tctatatggt atcctgggcg
       61 ctttgatatt aagcattttg gttttgaccg gcagcagaag tatgtggatc aacattggtc
      121 cgatcagcat tcagccatcc gaatttatga aaccggtatt tgttttgatt tgtgcgactt
      181 ccattcggga gcagcacgag aaaaagaaaa tcctgttttt ctatatttca aaggaaatga
      241 tgatcctcac aggtgcattt gttgtgatcg tcggtctgca gtggtggtgt cgtgacttgg
      301 gatcactgcc gacatttgcc gccgcatatg gctgtgcttt ggtgtgccgg ctttgttatc
      361 ccaaagctaa attttcgaaa aaaacgatta ttgtcatcag catcattggc gccgttcttg
      421 tcgcagcagc actgaaattc gctccgggct atgtacagga tcgtctctct gttgacattt
      481 ggaatcagat ggaaggaaac ggatatcagc agtgcaaggc tttgattgca atagcagaag
      541 gcggtctgtt tggaaaaggt cctggatgcg gttcgctaca caaagtcttt gcttcagata
      601 cagacattgt attctctacc atttgtgaag aatggggatt tttagtggca ttgctcgcaa
      661 tcttgattct catggcaatg atgctgctac ccatgataaa caaacctcgc agctatttcc
      721 acactacaat gattctggga tgcgtcgctg ttatgatcgt acagatgggg ctgaatatct
      781 ttggttcctg caatctgatc ccgtttacag gtgttacaat tccattcatt tctcaaggcg
      841 gcagctcgat gattaccagc ggttttcttg ccggtatgct gaaagcaggt cagtccccag
      901 tattcaaaaa gcctgaaccg aaaaagaaaa aattgaaaac gagaaaggcg gtaactgcgg
      961 catgaaaagt attcagagta tccgacgtgg agaacgcctg acaattttga ttctcgtgat
     1021 ttttttcatt ggaatgggca tccttgtgtt taaggttgtc cgggaatctt ctttctatat
     1081 gtctcattca gatgatgtaa ctcttggaaa agtttacgac cgaaacgacc aggtcttatt
     1141 tgatccggat gcgtccacag aaaactatga tgcaaactat tttctggacg taggaaatgt
     1201 catcggagac aacagcggtc aaatgacaaa cacgcttgtt tctgaaaaca tcgaaaagct
     1261 gcaaaattac agcttgattt tcggcgcaac gcaacacgga aaaactgcaa tttatgcaac
     1321 gctggatcat gaggcaaacc aaaaggtcta caatgctttt gggaacaaaa atgggactgc
     1381 gattgcatac aattatctga ctggtgaaat actggtttgt gtcagcaagc caagcgtcaa
     1441 cattttggac aactacagca acatcagtga actgccggat ggaagtctga tctgcaaagc
     1501 attttatgaa accacgccag gctcaacaca aaaaatcgca actacagcag ctgcgcttga
     1561 aacctttggt tacgatggct tgatgtcaaa atcgtatacc tgcaacggca tttataccac
     1621 gaagtacaac caacagatca aatgccatga tctgagcggt cacggcacag agaatatcct
     1681 gcagggattt gaaaacagct gcaatccatt ctttgcacag ctggtacagg atatgccatt
     1741 ggataacatc attcagactt acactcgcat gggcattgct gtcaatgatg ataaaatgca
     1801 aaaaatcaaa tttgacggac tttcctcttt cagtgcatct acaaaactaa cagataccag
     1861 tgattttgac accatgtgga gctgcatggg acagagtgag gcacttgcca gcccgctgca
     1921 aatgatgctt tgggaaagtg caattgccaa tgcaactgga aaaacaacaa tgccgtatct
     1981 gatcgatcac acgacaaatg taaccggaag tacaaactat tctgcgaaaa cctcttacgg
     2041 agacacaatc ttttctgcgg aagttgccgg cgaactcaag cagatcatgc ggcaaaatgg
     2101 acaagaccga tacagtgata tcggttatcc agtcggtgta aaaagtggta ctgcacaggt
     2161 gcagaacggc gcatctgaaa actctcttct tgttggtttc aacgatgaca gcagcttgcc
     2221 aatcgcattt tgtgttgtaa ttgaagatcg ggtttccgga gaagtttcta cttcaaatat
     2281 tgtttccacg atgctgagtg cattagatca taaataaaaa tacgatgggt ttcccatata
     2341 gaaaaaagac gtttcacaag cttaaatttg tgaaacgtct tttttagata gttgccggta
     2401 catcaaagca aattgatctt tttgaacata tcctgcattt tctgatcgat atccgcaatc
     2461 gtctctgagc agtctttcag ttctccggtc aaagattctg caattttcga atcaccagtc
     2521 gatttatacc gtagctgatg ttccagactt gcccaaaagt ccattgcaat ggtacggatc
     2581 tgaatctcaa ccggtgcaat ttcttttccg tttgacatat acaccggaac atccagaatc
     2641 atgtgaaaac tccggtatcc gtttggcttt ggatgcttga tataatcctt gattttccgg
     2701 agctgaaaag cgctgtgatc tgtcagaagg tctgcaattg catagatatc gtcaatatag
     2761 cagcatatga cccgaacacc tgcaatatct gtcaaattgt ttctggcact ttccggtgtc
     2821 acaggcatct cttttcgctg taattttccc acaatactct gcggcgtttt aattctgctt
     2881 tcaatgttgt gaatgggatt ccgctgaaaa cgcatactga attcattatc aaaaatgttc
     2941 agctgtgctt ttaccagttc aatggcagaa tcataaagat gctccaactc caaaaaagaa
     3001 tagaatacat cttgtatctg ttcttcttta tgttccggaa caacgtcacg aattgcatct
     3061 aagtaaaatt ccttatcatc catacagatc taaccctcct atacagaaaa cgcacacaaa
     3121 gcgttacttg tgtgcgtttt tgataaaatt gcggatcgtt tcttcaaaaa gcttacggca
     3181 ggagacgatt gatgtctctc gggaacatac ttgcctgacg aacattcgac agaccaagca
     3241 gtttcataac cagacgctcc aagccaatgc caaggccacc gtgcggcggc agtccatact
     3301 tgtgtgcctc tagatagtac ttgaagtcct caggatcaag tccctgtgcg tgcatcttat
     3361 ccagctgttc ctgataatca tggatacgct gtccgccgct tgtgatttcc agtccacgga
     3421 acaacaagtc aaacttgtat gccaaacggg aatcctcacg ggaattcatt gcatagaacg
     3481 gcgg
//
LOCUS       DAWENO010000199         3450 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9360_length_3450_cov_7.076289, whole genome shotgun sequence.
ACCESSION   DAWENO010000199 DAWENO010000000
VERSION     DAWENO010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9360_length_3450_cov_7.076289"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2768..2781
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          799 a          844 c          997 g          796 t
ORIGIN      
        1 aaaatggaat ttttttattt cagaaatctc ccgaaaggga aagcaagttc aaaggagatg
       61 atcgtatgaa ttatcatgaa gctgcggtac aggtgctgga gaagatcggc ggaaaggaaa
      121 atctgatctc cggcgcacac tgtgcgaccc gtctgcgtct ggtcgtaaag gacgagaaaa
      181 acatcaaaaa agaagagatc gaagagatcg agggtgtcaa gggcgtattc attgcccagg
      241 gacagctcca gattattttc ggaacgggtg tatgacgaat tcgtagccgc ttccggcata
      301 gagaccgcaa ccaaggaaga ggtcaaggcg gcggcagggc agaagatgaa cccgttcaaa
      361 aaggcgatca aaacgctggg cgacattttc gtgccgatca ttccggcaat tgttgccacc
      421 ggtctgctga tgggactgct ggaaggcttc agcaccctgt ttcccggaat ggcggcatcg
      481 gatacttata agatcttcca tatgttttcc aataccgctt tcacattcct gcccattctg
      541 attgccacca gtgcggcaaa agcctttggc ggcaatatgt tcctggggtc ggtaatcggc
      601 atgattatga ttcaccccga tttgatgaat gcctggaacg tggcatctgc ggtggaggca
      661 ggggaaacga taccgaagtt ctccgtctgg ttcggcttgt atgacgtaaa cgccgtggga
      721 taccagggtc acgtcatccc ggtcatcatt tccgtatggc tcatgtgttt cctggaaaag
      781 cggctgcata aaattgtgcc ggcatccatt gacttgttcg tgacgcccct ggtgagcgtt
      841 ggcgtgaccg gctatctggc actgactgcc atcggcccgg tattctctca gctggagacc
      901 tgggttctgg acggcgtgca gtggctgatc gctgttccgt tcggaatcgg tgcattcctc
      961 atgggcggtc tgtattcctc cacagttgta gctggtgtgc atcatatgta taccatcatt
     1021 gacctgggtc agctttccga gtacggcgtg acccactggc tgccgctggc atctgcggca
     1081 aatatggcac agggtgcagc ttgtctggca gtgggactca agagccgtgg caatcagaaa
     1141 atgcgctctc tggcgtttcc ggcagcactg tcctgtatga tgggcattac tgagccggca
     1201 atcttcggtg tcaacctgcg gtacatcaag gtgtttatct gcggttccat cggcggagcg
     1261 tgcggtgcgc tgatgacttc tatcttccat ctgggcgcaa cagcgaccgg tgttaccggt
     1321 atctttgcca tcctgctgca tctgaatcat ccgattcagt atatcgtttc catgctggtg
     1381 gcaatgggcg tagcgtttat tttgacatgg ctctttggct ataaggcaga aaaaccggcg
     1441 gcagagaaag ccgtttccaa agcataacgg aggtttcaga ccatgagtaa tgtgagaacg
     1501 atcaatctga aaaatgcctg cagggcgctg gagcgcagcg cagacaagga gacccggtgg
     1561 agaccggcgt ttcacctgtc accgatgacg ggctggctca acgacccgaa cggtctgtgc
     1621 cagatcggtg cgacccatca tgtgttcttt cagtattccc cgtttgatgt aaagccggga
     1681 ctgaactact ggggacacta caccacagag gattttgtga cataccagta tcacgaaccg
     1741 ggactgtgca gtgatgaaaa gtttgactgt cacggcgtgt attccggttc ggcgctggaa
     1801 gcggatgata ccctgtatgt tttttacacc ggcaatgttt tgcagcgtga tgacccggac
     1861 tatgactaca tcacccgtgg cagagagcac aacaccatcc gggcggaaag caaggacggc
     1921 attcactttg agaaaaagga agtgctgctg tacaaccggg actatccggc agatgtgacc
     1981 tgccacgttc gtgacccgaa ggtctggaag cacggagaca gctacacaat ggtgctgggt
     2041 tcccgccgaa aggatgatgt aggcgagatt ctggtttacc gttcttctga tctgaaagac
     2101 tggaaactgg cgaaccgtgt gacttcggag actcccttcg gctatatgtg ggaatgcccg
     2161 gactgtttta ccctggacgg tgtacggttc ctgtcattct cgccccaggg tgtggatgca
     2221 caggactgga aatacaataa tatttaccag agcggttact gccgcctgga gggcgacctg
     2281 tacggcgact atgaactgaa accgttccgg gagttcgacc acggctttga tttctatgca
     2341 ccgcagacat atctggatca gcagggacgc cgcattctga tcggctggat gggaatgccg
     2401 gactgtgatt acggctatcc ggaacgggaa tacggctgga caaatctgct gaccattccc
     2461 agagtcctgc accagagaga cggcaggatc ttccaggagc cgctgccgga gctgcaaaag
     2521 ctccgtggag aagaactgca catgacggtg caggacagtg cagagcagaa aaccgacttt
     2581 gtctatgagg cagagatttt gaacactgcc ggagaagaaa tggaagtaca gttcggcaca
     2641 gacggcagaa tcacctggaa agatggattc ctgtccctgg cgtttggcga aagcggtcag
     2701 aatcgtccgc tgcgccgggt gccgctggaa aagctggaaa atctgcgcat cttctgcgat
     2761 acatcttnnn nnnnnnnnnn ngttgtttgc caacgacggc gaggcagtgt ttacctcccg
     2821 gttctacgcc gatgccgcag catcgcaact gacggtgctg tctgcgcagg cggaggtgac
     2881 actctggaaa ctgcacccga ttcaaattga aaattgtttt tccaataagg aggcgtgaca
     2941 tgaaacaagt tttggcgatg ggcgaggcgc tcattgattt tattccggca cagcgtgact
     3001 gtctgctgaa agaagtagaa tcctttaccc gtgtctgcgg cggtgcgccg gcaaatgtgg
     3061 ctgcggctgc ggcaaagctt ggcggcgaaa gccgtatggt cacgcagctg gggaaagatg
     3121 cgttcggtga ttatatcgta gacacactgc aggatgcagg cgtggatacc tcctgcattc
     3181 tccggacaga tgaggcaaac acggcgctgg catttgtttc gctgaaagcg gacggcaacc
     3241 gggagttttc tttttaccgc aagcccagtg cagatatgct gctgcgggaa gaacagatac
     3301 agccggaatg gttttccaat gtggggattc ttcacttctg ctctgtctca ctcattgatg
     3361 cacctatcca gcaggcacac cggcgtgcca ttgcactggc gcaggaaaag aaagcgctga
     3421 ttagctttga cccgaacatc cggctgccgc
//
LOCUS       DAWENO010000200         3385 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9544_length_3385_cov_8.195796, whole genome shotgun sequence.
ACCESSION   DAWENO010000200 DAWENO010000000
VERSION     DAWENO010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9544_length_3385_cov_8.195796"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1454..1463
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          779 a          983 c          708 g          905 t
ORIGIN      
        1 gattcgatgc tgttttgtct gaaatttctg caaataaatg ggcggatttt acgaagaaat
       61 ctacatttca tagaaataaa ataaccatgc tgagaagatg tgctttccca gcatggcatt
      121 ttttatggtt ctggatttgt tccctgatac gctcttggct tgtatgcgtt gtgtcaattt
      181 caattttgtg tatttctgat cttatcctga cttgttcccg catacagaaa aaaggcatct
      241 cagctacctt tcaaatattc tccaaaaaga aaaaagtgcc gaaaaatcag cactttttca
      301 tctattatcc ttgcttcctt gacatcaata ccacgcactc aacgtgcccc gtcccaggaa
      361 acaaatccac cggctgcacc cgttctgccg cataccccat ccggctcagc attgcacagt
      421 ctctggctgc cgttgtggcg ttgcaggaaa tcatcacgat tcgctccggc tgcatctgca
      481 ccatcgccca gagcgctgcc tcatcgcatc cctttctggg cggatcgacg acgatcacat
      541 ccggcgcagt cccctgttcc gccagctttc cggcaatgct gcctgcatcg ccgcagaaaa
      601 acgccgcatt tttcacgcca gctctcgcag cattttcctt tgcattctcc acagcctctg
      661 gaacaatttc cacgccaacc aattccaaca ccgcatctgc catagacagc ccgatcgttc
      721 cggcgccgca gtacaaatcc aacagccgtt ctttccccgt cagctgtgca aattcttttg
      781 ccgcaccata aagccgctct gcctgtatgg tattcacctg gtaaaacgac tccggcgaaa
      841 gatcaacagc attgccgcac atggtgtccg taatggtatc ttttccccac agcgtttccg
      901 tggaatctcc cagaatcaca ttggttttct tcgggttgca gttcatgctg atactcttga
      961 tctgcggata ctgctcggtc aaaatctcaa ctaacggcat cagctttttc cgaattggct
     1021 tgcgcacgat aaagcaaacc atcacctctc cggaatgctg tccgcaccgc agatacaagt
     1081 gccgcatatc cccttttccg ctggattcat tgtatactga aatcgaattg cgccgcacaa
     1141 actccagcac tgtaccggca attttcccaa acaattccgg ctgcagcaaa cagtccttca
     1201 ccgaaatcac ccgatgactt ctccgggcaa aaaagcccag aatcggctgt cctggtttgt
     1261 ccaaagcgaa cggatactgc gccttgttgc ggtatctggt acgggactca gcgcccagaa
     1321 tcggcaaaaa ttccggagaa agctttccca gtctaataaa agcatcctgc accgcctttt
     1381 ctttcacacg cagttctgca tcatacgtga tatgctgaaa cagacagccg ccgcactggg
     1441 ataaaacgca ggcnnnnnnn nnncgcaggc aggcacaatg cggtctgccg aaggctccag
     1501 cagttcttcc acgatcccaa aggcatagct cttcagtact tttacgatgc gcacccgaat
     1561 ccggtcaccc actgcggtgt gcggcacaaa cactgccata ttgtccaccc gacagacacc
     1621 gctgccttcc gtggtcatat ccatgatttc tgtttcaaaa atgtcgttct ttttcaaatt
     1681 tccatcgcct cgtattgaaa aacctgttct gcattttcac atacccttgc acagaacagg
     1741 tttttgattg atttgcagcg caccggcggc tatagtacat ctcgcaccgg cacagcattc
     1801 ttgtttccca tcagaaattc gagctgttcc agccccaatt ctctgtacac atatagctta
     1861 cataaattct gtctgttgga atttttagtg tatccagcag aattccggta atgttgctgg
     1921 tcagcgtatt cagcgcattg gacgatgctt ttccgtacag ctgaacctct accattgctg
     1981 ccggcgcatc tgttccctga aagaaaagct gcttgtcgcc ctctgtctcc accatcagcc
     2041 agttctccgt ttttccggga actgctgtaa ttgcaagtcc aagatgtgtc ttaatatttt
     2101 ttgcctgttc gtctgtgatt gctgtatttg tctgaacatg aataaatggc atatctgtac
     2161 tcctttttac tccgtttcct gttcccgaat ccgctcgcag atctcccgta cctgctcttt
     2221 cttctgcatc agctggtcaa actgacggat atactcatag ggaaacttat agttatggat
     2281 tcgtgtgatt ctgccgcagg gttctaccag ctttgtggac gcaccgcatc cagcgccaaa
     2341 aatggactga ctctcatcca taataaggat gttgtacagc gattcctttc ctgcacgggc
     2401 atatcccacg ttttcctggt tgtcaatggt gttcttctgc cggtacatat aatagggcgc
     2461 atagccgtgt tccggcagac ggcgcatact ctcctgcacc atctgttcca ccgggttatg
     2521 tacctgactc tctccctcag caaaaagatt tgccgcccgt ttcagcgtca gcgcatgaac
     2581 agtaatgctg tccgggtcta acgccaggat cttctccagg gtatcatgaa agccctccgg
     2641 tgtatcccct ggcagtccgg caatgaggtc catattgata ttgtcgaatc ctgccgcacg
     2701 agccatccgg aatgcctcca ccgtctgcgc cgtagtatgc tttcttccaa tagtttccag
     2761 tacgctgtcc tgcatagtct gcggattgac agaaatacga tcaaccccga accgtttcag
     2821 tgttttcagc ttttccgggg taatggtatc cggtctgcca gcctcaacgc agtattcccg
     2881 cagatgcgaa aggtcaaaat tgtcctgcac agccgtcagt acctgttcca gctgttccgc
     2941 cgtcagcgaa gttggcgtac cgccgccgaa atagaccgta tccaccagaa gttcctgttc
     3001 ccgtaccagc tgcccccaga tcaccagctc ttcgcacagc ttttccagat atcccggaat
     3061 cagcttgtgc gcctgttcta tggaatggga tacaaaggaa caatagctgc agcgtgtggg
     3121 acaaaacgga atcgacaaat acaaactgat gccacgcttt ggcgttttct ccaaaagcgg
     3181 ctgctgtacg atcgccgtat cgtagaccaa agacagcttt tccggggaaa tccagaactt
     3241 ctcatggagt ttctcacaga cctctgcctt ggtataccct ttgcccagat aaggctgcac
     3301 cttttttacc ggacgaatcc ccgtcagcaa gccccacgcc ggatgcagat ctgtgatctg
     3361 ttccagcagc cggaacagca tggca
//
LOCUS       DAWENO010000201         3333 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9700_length_3333_cov_12.019524, whole genome shotgun sequence.
ACCESSION   DAWENO010000201 DAWENO010000000
VERSION     DAWENO010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9700_length_3333_cov_12.019524"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          642 a          963 c          932 g          796 t
ORIGIN      
        1 ctagagtttt tgtgtttttc tcagaaatgc agataatgtc ccatcttgaa ctgcttggtc
       61 ttgagatacc gcaggttctc cggcttttcc ggaatgtcaa tgggaacacg ctcggcaact
      121 tccagtccgt attcgcccag ttctgcgatc ttttccggat tgttggtcat gagccgcagg
      181 gaagtcacgc ccagatgctt cagaatctgc gcacctacgc tgtaatcccg gagatccggc
      241 gcaaagccca gcttgatgtt tgcctctacg gtgtccagac catcctcctg taacttgtac
      301 gcccggatct tgttcagcag accgatgcct ctgccctcct ggcgcagata gaccagaacg
      361 cctctgccct ctgcgtttat catgtccagc gcacgattga gctgttcgcc gcagtcgcac
      421 cggcaggagc cgaacacatc gccggtcaga cactcggagt gtacccggca caataccggc
      481 ttgccgtccg acacatcgcc catgaccagg gcaacgtgtt cgtcgccgtt gatagtgttg
      541 cggaaaccga acatctggaa atgcccgtgg acggtgggca gttctgcccg tgccacttct
      601 tccacgaagt tgtcgtgatt gcggcggtag tgctggagtt cccggatggt gataaaggac
      661 agaccgtgct gttttgcaaa gtccgccagc ttttccccac gcatcatggt gccgtcgtcg
      721 tccatgatct cacagcagag acccacctgg gtcagtccgg caagacggag caggtctacc
      781 gtagcctcgg tgtgaccgtt ccgttccagc acgccgccgg gctttgcctc cagcgggaac
      841 atatgtcccg gacggcggaa atcgccggga tgcgccgccg ggtctgctgc catgcgtgcc
      901 gtcagaccac gctctaccgc agaaatgccg gtagtggtgt cgatgtggtc gatggaaacg
      961 gtgaacgccg tttcgtggtt gtccgtgttg tgggaaacca tctgggtcag tcccagccgg
     1021 acaatgttgt catggctcat gggcatacag atcagtccct ttgcggcgct tgccatgagg
     1081 ttgacgtttt ccgtggtggc gaactgtccg gcgcagatga ggtcgccctc gttttcccga
     1141 tccggatcgt ctgtcaccag aatgcacttg ccgttccgca gatcctgcaa agcttcttca
     1201 atggtgttat atgtaattgc catagaaatg acctccttgg ttgtgtaggt tatggctcat
     1261 tttgttgctt taattttgtt gccttaaaag aatccgttct gttgcagcag ttccatagtg
     1321 acgccgccgc ttttgggctg tatcagcttt tccacgtact tgccgatgat gtcggtttcc
     1381 agattgacga tctcaccgga gcgatgtttc agcagggtgg tttcctcacc ggtgtgagga
     1441 atcaggctca cggtgaaatc cgtgtgggaa acctttgcca ccgtcaggct gatgccgtca
     1501 atggcaatgg agcctttttc caccacatac cggagtagtt ccggtgcggc actgatcgtc
     1561 accagcactg cgttgccctc cggctgcatg gaacgaattg ttcccgtgcc gtcaatgtgt
     1621 ccggcaacaa tgtgcccacc gaatctgccg tctgccgcca tggcacgttc cagattcact
     1681 ttcgagccgg ttttcagctg ccccagattg ctccgtcgca gggtttccgg catgacatcc
     1741 gccgtgaagt ggtcttcgcc catgcgaacc acagtcagac aaacgccgtt cacggcgatg
     1801 ctgtcgccga tggcagtgcc ttccaatact ttggatgcgg tcagttccag ttcgcatcgg
     1861 ttcggtgcct ggttcagccg ccgcaccgtt cccatttctt ctacgattcc ggtaaacatc
     1921 agcatgactc ctttcgggtg tattccagca gcagatcctg ccccagccgt gtcaccgtgg
     1981 gtgcagacag aagaactgcc tggtccgggg tggtgatgcc catgccgccg atgggggaca
     2041 gaccgtctcc gccgaacagt ttcggtgcga catagacctg caccaggtca cagcagccct
     2101 cctgtaatgc cgccgcatgg atggattcgc cgccttcgat caaaatgctg tccagtccac
     2161 gctgtcccag ctgttccagg cagttccgca gggaaatgtg tctgccgtgc agatgctctt
     2221 tcggcacggg aagattttcg tatcgggcga ggagcgattc caggtctgtc tggtgcagca
     2281 gttccgcctc cagaacagtg acaccctttt gttccagggc tgcctttttt tccttgtccg
     2341 gggcgtggca aaagacgatg accggaactt cgccggcagt ctgtaccagc ttgctctcca
     2401 tgggaatgcg cagccgggtg tccagtacca cacgtaccgg ctgagacgga ttctcacagc
     2461 ggcaggtgag catggggtca tctgccagaa cagtgccgat gcccacgcag atggcagcca
     2521 cacgctttct cgtctgctgg acgtgattcc gggcagcctc acccgtcacc cacttggacg
     2581 caccgccggc gcaggcaatc ttgccgtctg cggtcatggc gtacttcaaa atggtgtagg
     2641 gcatcttcgt ggtgatgtac cgaaaaaaga tggggttcag ctggtcgcac ttttcccgga
     2701 gaaagtccgg gaaaacctcg atgccgtgtt cccggagaaa cgccacgcct tttccggaga
     2761 ccagagggtt cggatcacgg gagccgatgt acacccggcg aatgccgctt tccaccagtg
     2821 cctcagtgca tggcggctgc ttgccgtggt ggcagcatgg ctccagggtg acgtagatat
     2881 ccgcaccggc agggtcttcg atgcagtgct tcaaggcgtt tcgttctgcg tggagatcgc
     2941 cgtatctggc gtgccagccc tcaccgatga tgcggtggtc tttgacgatc accgcaccca
     3001 ccaggggatt ggggttggtg aaccctgtgc cacgccgtgc cagctgaatg gcacgttcca
     3061 tataaaaaga atgctctgtc atgaaaacac ctccaaaaaa tgccctgttc tgcaaatgca
     3121 caacagggca ggaaaaacgg cttggaaaga tgccgttatg attctggatt ctgggggaaa
     3181 cacgtaaaat tgattttgcg tggcttctct catccggact ataccgtcgg tttcggaatt
     3241 gcaccgaatc agccagtttt cgaaactggt tcgcagactg cggcaaaaac gccgttcact
     3301 gccggttctg aattacacag taccctgaag ctg
//
LOCUS       DAWENO010000202         3317 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9750_length_3317_cov_9.248314, whole genome shotgun sequence.
ACCESSION   DAWENO010000202 DAWENO010000000
VERSION     DAWENO010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9750_length_3317_cov_9.248314"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    718..727
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1090..1099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          881 a          786 c          985 g          645 t
ORIGIN      
        1 atctcgccga tggtctgccg ttcgtagtaa tattccacag acagcagtcc acgcagcgca
       61 tagtaagcgg tatccgcacg ggatgccaca tcacgggtga tgcccagttc cttataaaag
      121 ttcatgatag agcctgggac aacgctgtgg aacgtccgca tacagggcag ccgccagaac
      181 atcggatagt tgtcgcagtc ctctgaaata tcgatgcgga agaagttgtc ctctgagacg
      241 gagaccgaga tctcctgttc cgggtcgatc gcctggtcaa cataggttct cgcacggaac
      301 ggctcaaatg cgccgaaata caccactgcg gatgtgcagg caatgcagca ggctgtggtg
      361 aggacaagtg cctgtttcag gaaaggcttg cgggcttttc tgcggcgcag cagatagatg
      421 gtgagtacca gtccggcagc gctgataagc agcaccaggt aaaaatggaa cggatattcc
      481 gcaaacgaaa accagtgtat cttgtcgtcc tctttggtgg gcagcaggga gatgagtccg
      541 aacgctgcca gaaatccaaa gcaaatgcgg atgccctgtt tccaggagat ctgacggttg
      601 tccaggctgt atgcggtcat catcgccaga atgagaatcg gcatatagaa ccagcgtgca
      661 taatacgaac cattcagcgc atagaatgca ctgttcagaa tcgggacgca ggcgcagnnn
      721 nnnnnnnggc tcagcagaat gcgccagatc cgggtgcggt cgttgtaggc gatcatgtcc
      781 atgccgaaca gatgctccga gacccggctg tttgccagga tcgccagggc agccggcagc
      841 aggatcacac aggcgatcat gacaccgatt accgcctcca gcagcaccag gaagaacttc
      901 cgcagggttg cgtgaaagtc cttggagaaa cagcgcacaa tgaagtacag taccagaaag
      961 accacttgtc cggtaaagaa aaaatagttg atgatggcga ggagtgcgac tgacagtgca
     1021 aacacgcccc ggcggttctg gttgaccagt tcttccatgg cgatgagcag cagcggaaag
     1081 aacgctgtgn nnnnnnnnna aagccggaaa atgcgtagag cataccgccg atcagcgccg
     1141 cattgggatt ccggacaaaa cgccggatat aggcgtaggc agtcagcgag gcgatgccgt
     1201 gcttcatgca gagcagaact ggcatggcga atgtgaccca gctccggggc aggatgacag
     1261 agagccagaa aaacggactg cccagaagat aaaaggcata cgagccgata aagttgacgc
     1321 ccaggtcggt gtaccagttc cagccaaagc tgccgctgcg gacagcgtca ttcgccagat
     1381 tgtaaaacgg gatctgctgg gagttatagt cgccgtagta gatataatag ccgccgtctg
     1441 caatgataat gggcagaatg accagcagga gactgccgaa tcccagcagg aatgccagaa
     1501 gatagcggcg catttcatcc cggaggacag ggcggcggag tgtgacagat ttcatggaag
     1561 ggactccttt gtgtcaaaat gtggattgct gcatagaatg aaatgagacg gtcaaaaccg
     1621 tgtttttgtc tccaggaaca ccacattggt catgacaccg gcgctggaaa cgtccaggat
     1681 accataagaa ggcggatttc cgtctctggg gcaggaggcg ctgccgggat tcatgacata
     1741 gatgccgttt tcataggtgc attgggatac gtgggtgtgt ccgtacagtg caatgtcaca
     1801 gccctcttcc agggcagcct gttccaggta gtccagtgtg gagttgaccc gatagcggtg
     1861 accatgggtg gcaaagacac gatgtcccgg catcagatca atggtctgtg tcagcggtgc
     1921 ttcactgccg tagtcgcagt tgcccttgat atagcggaac ttgtcagcga cctccggcat
     1981 attccggcag agcagctgat attcgctttc gccgtctccc aggtggatga acatatccgc
     2041 atccgggtgg cgcaggacga tggtttcaaa attccggtaa tattggtgtg tatcagaaag
     2101 aacgataatt cgcatagtga cctcctagaa aaaatatcat cttttattat agcacaattt
     2161 catggagatg gcaacttttt ttgaaaagtt ctgaaaggag cattgtttat ggaacgcaag
     2221 aacatgaatc cacagcaggt cgatcagctg ttgcaggcag ccagcaaaaa actgggtgtg
     2281 ccgccggagc agctgcgttc ggagttggag tccggaaagt atgacaaagt gctgaaaaat
     2341 atgagccaga aggattcggc aatgctgcaa aaggtgatgc agaatccgaa gatgatggaa
     2401 aaactcatca gctcgccaca ggcgaaggcg ctgtatcaga aactttccgg gaaataagaa
     2461 gaatacacat tctgcaaaaa cggcagccgc agtttccggc tgcccgtaca tgaaaaggcg
     2521 gtgaaagcgt gtggacgata tgctgggaaa aataggagct atcctgtctg atccggagag
     2581 tgtacagcag ttacaggagc tggcgcagat gctccagcag gagagcggcg agacagggga
     2641 atcaccaaag caggaacaga gcggaaacgc tgacggtgag agcggcggtt tcgatatggg
     2701 tatgctgctg cgcatccagg aactgatggg agcaatgcag cagaatgatg aggatgcccg
     2761 gctgctgctg gcgctgcgtc cgcatctgaa aacacagcgt cagaaaaagc tggaccaggc
     2821 ggtcaaactg atgaaactgt atgccgtatt cagtgccgtg aaagaaaacg gaatgctcca
     2881 ggatctgctg tgatcgggaa ctgaaccgga ggaggtgaga aggatggcac aattttcaga
     2941 gcaggaacag aacgccatgc gccgggatgc ggcaaggcgt gcgcaggaga tgcagcggcg
     3001 tgtgcatccg accgcctcgc ccgggagacg gctgccgccg cagtttgcag attatccgcc
     3061 ggaaacgtct gcaccggagc cggaaccgac accaaagccg cagccgccga aactgccgct
     3121 tttggggaat ttgccgcagt tcaatcccgg cggacttttg tccaatctgg atggcgacac
     3181 catgctgatc cttgccatga tggtgatgct ctataaagac ggcggctggg atggctgcga
     3241 caaaaagctg ctcatggcgc tggcatatct actcacataa aaggagaaat gaaagatgca
     3301 agaagtgatt ttttggg
//
LOCUS       DAWENO010000203         3308 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9785_length_3308_cov_12.667077, whole genome shotgun sequence.
ACCESSION   DAWENO010000203 DAWENO010000000
VERSION     DAWENO010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_3308_cov_12.667077"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          565 a          937 c         1033 g          773 t
ORIGIN      
        1 aaggcacttt ttccccggac acatcccggc ttttgcctgc tttttgcagg acggcagccg
       61 gaagtttgcg gtaggaccgc ccacgtcgtg gatatagccc ttgaagttgg gcatttcggt
      121 gatctttttt gcctcttcca ggatggattc ctcgctgcgg caggtgatgc gccgtccctg
      181 gtgcagggcg atggagcaga agttgcagta gccgaaacag ccccggttgt gggcgattga
      241 gaactgtacc tcttcgatgc ccggaacgcc gccacggctg tcgtacatgg ggtgcggtct
      301 gcgctgatag ggcagccggt agactgcgtc cagctcgtcc tgggtcagac tcagtgccgg
      361 cggattctgc accagcatct gactgccgtg gcgctggaga actgtctttc cggtgatctc
      421 gtcctgctgg tcatactgct gccgggtcgc cttggcatac gcctttttgt cccggcagac
      481 ctgggcataa tccggacact ggacaccgcc ccacggggtg ttttccggct cggtgaggta
      541 gcacgtgcca cggatgccgg tgatctcccg gatgggaacg ccgtctgcca gctgctgtgc
      601 cagttccgtg atctgatgct cgcccatgcc gtacatgagc aggtctgcgc cgctgtccac
      661 caggatggac gggcggacgg tgtcgtccca gtagtcatag tgggcgaacc gccgcaggga
      721 cgcctccaga ccgccaatag caatggcggc gtcgggataa gcctcccgga tcttccggca
      781 gtaggtgata acggcacggt cggggcgcag tccggctttt ccgccgggag agtacatatc
      841 actgctgcgt tttcgcttgg cagaggtgta gtgggcgacc atggagtcga tgttgccgcc
      901 ggtcaccaga aacgccagtc tcggcttgcc gaaacgctga aagtcccgga cggtgtgcca
      961 gtcgggctgg gagaggacag cgacgctgta gccctgggat tccagcagcc gggtgataat
     1021 ggctgccccg aaactggggt ggtctacata ggcatcgccg gtgatgtata cgaaatccgg
     1081 ctgggatacg ccggtttcca gaagttcctg catggtgaca gggagaaagt gggtcatgta
     1141 tttgctcctt gtaaatttgc cttccttgaa aatcgcctat cctatagggt gtgccttttc
     1201 gaggaaggct tattgtcact cattatgtcc cccttgaaaa gggggatgtc ccgaagggac
     1261 agggggatta taacaaagaa aattttcgtc atttattgca gccgtcgcat tttccgttct
     1321 gccagctgtt ctttcgtcat aagcaccttc cggggctttg cgccctcgta gggaccgatc
     1381 acgcccatct gttccagctc gtccatcatc cgggcggcac gggcatagcc tagttttagc
     1441 cgccgctgga gcgatgaggt agacgcctgt ccagcctcaa caaccacttc gatggcacgt
     1501 tccagcacgt cctcatcgct gcccggttcc ggctcgttga cggcagccgc tttttctgcg
     1561 gcacgcagtt cctttgcctc tgccttggtg acggggatgc tctgttccac ctgttccatg
     1621 atttcgtcgc tgtattcgct ctgggtctgg gacttgatga agtccaccac tttttcgatc
     1681 tccttggtgg aggtgaaaca gccctggaca cggaccggct tgatctttcc gttgggtaag
     1741 tacagcatat cgccgtgacc cagcagcttt tctgcgccgc cggtgtccag gatggtacgg
     1801 gagtccacct gggacatgac cgacagggcg atacggctgg ggatgttcgc tttgataagt
     1861 ccggtgataa tatccgtggt cggacgctgg gttgcgatga tgaggtgcat tcctgcggca
     1921 cgggctttct gcgccagacg gcagatggag tcctccactt ccttggaagt ggtcatcatc
     1981 aggtcggcaa gctcatcgat aatcaccacg atttgcggca tgggttccag accgctgccg
     2041 ggctgtgccg ccgcagcgtt gtagtcgccc aggtcacgga cgctgttttc tgcgaacagg
     2101 ttataccgcc gctgcatctc ctgcaccgcc cagttcagcg caccagcagc ctttttcggg
     2161 tcagtgacca ccgggatgag cagatgggga atgccctcat agaccgtgaa ctcgacgatc
     2221 ttcgggtcga tcagaatcag cttgacctct tccggtgtgg cgtgatacag gatgctcatg
     2281 ataatggagt tggtgcagac cgacttaccg gaaccagtag caccggcgat gatcatatga
     2341 ggcagccggg cgatgtcgcc tacgacggca tttccggcga tgtcccgtcc caccgcaaac
     2401 gccagcttgc tcctggaact gcggaaactg tcgctctcca ggatttcccg gagcgaaacg
     2461 gtgtccttgt ggctgttggg gacttcgatg ccgatggcag gctttccggg aatgggcgcc
     2521 tcgatgcgaa ccccctgcgc tgccagagaa agggcaatgt cgtctgccag tccggtgatc
     2581 ttggagacct tgatgccggc ggcaggctgt atctcatacc gtgtgacgga cggtccccgg
     2641 aaaatcccgg tgatgcggac ggtcacgccg aaacttttca gcgtctctac cagaatgccg
     2701 gctttttctt tcatttcctg gtctaccgca gggtcttcgg cacggctgac gccgttctgc
     2761 aaaaagtcga tgctggggat ctgataggga cgtgccggcg gctgggagac cggttcggat
     2821 gccggtgcag ccggtgctgc tgtttctgcc ggagcagttt ctgtatccgg ggtgaacagt
     2881 tccggcggat cctgcatcag ctcttccggg gagacgatcg ctccgttgtc cggctcgtcc
     2941 tccgggatgg gaatgtcgat gtccatgtca tacgccgggg tatagggcgc attgtcctgg
     3001 ggctggctgt agttgtcccg tatcatagcg tccagatccg gggaaagcgg cgagttgtcc
     3061 tcgacaaaat cggggatggc agattccagg ctgtcgtctg ccgccggttc gggaactggt
     3121 tcggcaggac ggctgccctt gcggtggacc tttgcccgtt tcggtccggt ctgctccggg
     3181 agcagaaatt ccggctcttc tgcctggggc agttccggtt cgccggagat gtccgtgtcg
     3241 gggatatcgt ccgcccagtc cggctctttg ttccagagtt cccggagctt gcggaacggc
     3301 cagcagat
//
LOCUS       DAWENO010000204         3296 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9829_length_3296_cov_12.258254, whole genome shotgun sequence.
ACCESSION   DAWENO010000204 DAWENO010000000
VERSION     DAWENO010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9829_length_3296_cov_12.258254"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          802 a          840 c          835 g          819 t
ORIGIN      
        1 tgcaatggcg atacgctgtt tctgtccgcc ggaaagctgg ttcgggtact ggttcgcctt
       61 gtcggaaagt cccacctttt ccagcagctc agcggctttc tgatctgcct ccgcctgtgt
      121 catacgcttg agctttaccg gtgcaagggt aatgtttttc tggatggtca gatggggaaa
      181 caggtggaag tgctggaaca ccatgcccat tttctgccgc acggcgtaaa gctgacggtc
      241 aggcatatgt gtcaagtcct cgccctcgaa caggatctgt ccgccgctgg gctgttccag
      301 gcggttcaga caccggagaa aggtactctt accggaaccg gacggtccga taatgaccac
      361 tttttcgccc ttttcaatgt tggtggaaat gtctttcaga atgtggagat cgccgaaaga
      421 cttgtccaga tgtttaacct cgatcactgc gtgccagcct cctttccaga cgtgttacca
      481 gagaactcag aatcatgacc ataatcagat agatcagcgc aacggcgatc agtggcaaga
      541 atgcctcata ggtctgactg cggatgatat cgccgccttt ggttaagtcg atgaccgcaa
      601 tatagccggc aacagaggtc tctttcagca gtacgatgca ctcgtttgcc agtgccggca
      661 gcacgttctt gaacgcctgg ggcaggatga cagagagcag tgtcttggga tagttcagtc
      721 ccagactgga gcttgcctca aactgtccgt tgtcaatgga catgatgccg gaacgcacga
      781 tctctgccac atacgccgca gagttcaagc cgaatgcaag caccgccaca aaggtttttc
      841 ccacattcac cgaggcaaaa ataacaaagt agataatcag cagctgtaca acgacaggcg
      901 ttccacggat cacagtcaga tagatccgtg ccaggacatt cagaaatccc agctttccgg
      961 tcttatcatg ggtcgagcgg atgatcgcaa tgagaaagcc cagcaccatg cccaggatca
     1021 ccgcaaaaaa tgtaatcagc agcgtggtct ttaagccgtc cagcaggtac atccagcgat
     1081 ccttctcgat aaacgtagag tggaacttag ttataaagtt atcaataaaa ttcacggccg
     1141 ctgtctcctg ttagtctttc cggacaatga tgacctgtgt tgtggtcgca tagccctgtg
     1201 tgaaagatac gttttcttca cgatccggag ttacggtcat gccggctacg ccaacatctg
     1261 ccttgccgga ctgtacggca gcgatgatgg agtcgaattc catgttttcc actttcagtt
     1321 ccatgccgat ctcatcgcag acagcctgca tcatatccac atcgacacca acgatggtat
     1381 ctccgtctac gctctcatac ggcgggaatt cagcgtttgt tgccataacc agtgttccgg
     1441 tgcgctctac gctgtcatcc cgtgtgtagg actgctggtc tgcatcgtca ccgatccagt
     1501 ggctgacgat ctcatccagt gtgccgtcct ctttcagctt ggtcagtgcg tcgtccagct
     1561 tctgtcccag ctcatcattg cccttcttat aagcgatagc atattcctct tctgcaaacg
     1621 gatcgtccag aattttcagc gtatcgttct ttgctacgaa tgcctttgcc ggctcgctgt
     1681 cgatgataac ggcatccagc ttgccctgct tcagtgcctg cactgcgtct gcacccttgt
     1741 tgtacggctc aaccttggca tcctcgacat cacctgcata aatataaccg gtcgtaccca
     1801 gctgtgcgcc gatggtcttt cccttcagat cgtctgcgct gaacacggtg tttgccggtg
     1861 tgccgcaggc ggtcatggat acggcgagca ttgcgcttgc tgccaggagt gcgatcgttt
     1921 tcgttgcttt tttcatacaa aaaacttcct ttctgtcagt ccttttgctg acacggctaa
     1981 tattgcttta ggcaacaccg cacaaagctt gtgcgtaaga tgcgaagccg gatttttcgg
     2041 cagattgcac agaaattccg caggcatact gccgtatgtc aagggatttc tgtgcagtat
     2101 gacggaaaat cagcaagcag attgcgtgca aagccgctag gcgggctttg tgcggtgttg
     2161 cctttaatgc aattcttttc tattataatc gaaaacggga aagaatgcaa gtcattttcg
     2221 cccttttttg atttttttgc gtttttgcag tcttatgttg ataaattctt tcattttcca
     2281 cgtcaaaccc ggttgacaga accggaaaac catgttataa taaaaactgt ttttgtaata
     2341 atgtagtagc gccgtaaagc gctgagaaac tgctctcaca cgatgagaac aactgcatga
     2401 cagaaaggca tcccgtcttc cggcaggatg tattctgtac caccacacgg atcagaaaaa
     2461 aggagcgttt ttggtatgaa aaagaactgg acaacacgtc tgatggcagg cattgcagca
     2521 ggcacaatgg tattttctct ggcaggctgc ggcagcagca gcgacggcaa gaagagcgcc
     2581 gacaaggaaa agtttatcgt tggctttgac gctgcatttc cgccttacgg ctatcaggat
     2641 gaaaacggcg aatacgtggg attcgacctg gatctggcgg cagaggtctg caagcggaac
     2701 gactgggaac tggtcaagca gcccatcgac tggaatgcaa aggacatgga gctgaactcc
     2761 ggcaccatca gctgcatctg gaacggtttc accatgaacg gccgtgagga tgaatatgcc
     2821 tggacagacc cgtacgttga caactcccag gtatttgtgg tcaaggctga cagcggcatt
     2881 gccacatttg ctgacctcgc cggaaagagc gttgccgtac agacagactc ttccgcagag
     2941 gctgcactga aagaggacga caacaaggaa ctgaaggact ccttcaagga actggtcgtt
     3001 gtctccgatt acaacaccgc attcatgaac ctggattcca atgcagtcga ggcaatcgct
     3061 atggatatcg gcgttgcaaa gtatcagatg gaatcccgtg acggcgaata caagatcctg
     3121 gacgacgttc tgtccgcaga gcagtatgcc gttggttttg caaagaacaa cactgagctg
     3181 cgtgacaagg tgcagaaaac actggacgaa atgcgcaagg acggcaaatt tgctgagatc
     3241 gctgaaaagt gggatctgac ggattctgtc attgactaaa tctccgaaag gaatct
//
LOCUS       DAWENO010000205         3269 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9908_length_3269_cov_9.395146, whole genome shotgun sequence.
ACCESSION   DAWENO010000205 DAWENO010000000
VERSION     DAWENO010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9908_length_3269_cov_9.395146"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2375..2384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          735 a          908 c          734 g          882 t
ORIGIN      
        1 ttgcgcagat atgtgtaata cgccaggtcg ttttcaccgt tcatctttaa gggacgcatt
       61 cctttccgct ccgtccggat gggattcata atgcgaaccg catccacctt ccacaaaatg
      121 gtcggcttaa agtaaatgct gtgtacgatc cctttgattg cctcataggt cgggatgtca
      181 taggtacact tttcgccgga cggtctcgtc agcacatcgg aaaacagtgc gtagtctccg
      241 cacacttcaa actcaatggt atttctatga atcgtctgac tcatactgca gcctcctttc
      301 cgcaatttgt tccggaaaag cacaccaggg tattttttcc ggaattacaa ttcaattata
      361 tcacatcttt acacaatcgt caacaatttt agatggattt atagtcgatt cgacaatatt
      421 cgacaaaaat tccaatattt tctgtacatc ataaatccaa aaacggacgt tcctgtccct
      481 ctgtcagcag tcccagcgct gtcttgtcat agaagttctc ttccagtgcc agcgctccgc
      541 aatgcagcgg acagagtgcg cctgcctctg ccagtttccg tttgtccccg gcgtaaactg
      601 aaaccgtata cttttgcacc gaccgcagca gtttctgcat ctgtccgaat tccaggtcgc
      661 cgttcagatc ccggatcatc tgcccggcat cttcatcata cggcacaatc acgctttctc
      721 cggcatcctc aattacatga aacgcttttc cagctgtggc aaacgcctgt agggaaaact
      781 tgtcatattt ggagcggttt ccttttccca gttctgcccg tttgctgtcg gtggaaagca
      841 gtttcagcag atcctggtcg ttttcggact gcggaaagga cagttccttt tgattctcct
      901 ggtagagttt ctggaaatac tgggacaggg tctccacgga cagcagatca gtctccttct
      961 ctttctgctg catacagaga gaatcccaga cagcgctggt cacattctgc gcagagacga
     1021 tttccttcag ccggtcaatg ttttcctctg tcagtttcat cagatacacc ggacagatct
     1081 ttctcatttc cccgtgccgg ttgcaccgtc ctgccgcctg cgccgcattg gataatccag
     1141 ccttagaacg caccacgcag ccaaaggaaa tgtccacccc tgcctcaatc agctgggtag
     1201 tcacacagat gacgttttcc ccgtcctgca aatacttccg catttcccgg atggcgttct
     1261 cccggtgtgc cggacacata ttggtactga gatgaatcag atggacattt tcttctccgt
     1321 cctccactcg ttctttgaga cggcggtaca ggtcagctgc ggttttccgg gtattgacaa
     1381 ccatgagaac actgcgatgc accttgaact gttcccagca gaaatccgcc gcctgtgcat
     1441 tgtcatagct gtccgtgaga tattccagtt tcgtccgctg aaatacagaa aagtcctcat
     1501 gatagtctcc ggtcatgctc tcctgtgcat ccagcaaaat gggatatttc tgcttttcca
     1561 gcagtggttg tgtcgccgaa caaagcacca ctgcacagcc gcagatacgg gacagaaagt
     1621 tcatcgccag gttgaacaga taggtacatt tcagcggaac ggactgcacc tcgtcgatga
     1681 gcagcaccgc acggcaaagc cggtgcatcc ggcgcaccga agtgttcttg ccgtcaaaca
     1741 gtgcctgcaa aaactgcacc tgcgtcgtgg caagaaccgg cacatcccat ttttccgtgc
     1801 gcagctcata cttgcgcagt tcctcttcca cttcctcgct gtcatcaaag accacgttgg
     1861 agtgatgctc cagaaaattc tccgccccgg aaatttcccg gaaatcctgg ctgttctgtt
     1921 cgagaatgga cataaacggc gctgtgtaca cgattttctc gatgccgttt tccggacgca
     1981 ttgcatagcg aatggcaaat cgcagagatg acagggtttt tccgccgccg gtgggaacga
     2041 tcagacggca gattcccacc ggatgcgccg caaattctgc acagcgccgg gaaatatcct
     2101 gccgctgtcg ggaaatcgca tccgtcctgc cggaaaatcc gtccagcttt cgctgcatcg
     2161 ccagctccat ccgttcccag acctgcgccg catcataatg cttttccgtc tgacaatcat
     2221 tcatgaagtc agcggtgtcc acacagtcgg catccatcag aatcgactgc aaaaggcgtt
     2281 ccagcattcc cagataaaat gtgaattctg tcctgcggtt ctgcttttcc gctttgtttc
     2341 ctgcatcaaa tgccttgcgg ttttcactgc gcagnnnnnn nnnntccttg atcctgcggt
     2401 acactgccgc aaactcttca ctggcatcct ccagcagtgc tttcagtgat gcttccgaat
     2461 atcccatttc cgcaagcatc gcatccagat tcggctcgat ctcctcatag cgttccacct
     2521 tggcgttgcg gattcggaag tagtcgtctc cgctctccgt cagccagtcg tggagtccgt
     2581 ggtgtgacag aatcgtccga gcgatcagac gggaggtgcg gtacggcatc tcctgatccg
     2641 gaaagtgccg gtctgaaaag gcgcagagat actttgcccc ggcatagcag tggtcgatct
     2701 caccacgatg aaagcggctg tctccgtgga tgtacgcata gaaatcccgg ttcagctttc
     2761 cggcatcgtg aaagattccc tgtagtcttg ccagctttcc cagccccacg gattccccgt
     2821 atgattccgc cagcgccgcc acatttcggc agtgttcggt gacggactgt tccatgccat
     2881 ccgattcccg gatatgtgca gtaaaatcca tttcagattc cctcctctgg tatctgtttt
     2941 ttcgatttct tcgatttcat agttttatta taccacggca gtttttcaaa tacaataaaa
     3001 ctgcttgtcg aattttcgac aattgaacga ttgcttcgca atggtgaaac gatttcacat
     3061 tctccggcaa acgcaccata tttatgaaac gttcccttac ttttttaagg aaaaacgcta
     3121 ttgacagatg ttgtaaaata ccctataatg aacttagagt ttggttcgta ttttttatgt
     3181 gcggattttc gagcatgatt ttgtcggtga caaggaaaaa atccgcaggc atactatcgt
     3241 atggcaagga tttttgacgc agtcagcgg
//
LOCUS       DAWENO010000206         3263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9931_length_3263_cov_8.502805, whole genome shotgun sequence.
ACCESSION   DAWENO010000206 DAWENO010000000
VERSION     DAWENO010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9931_length_3263_cov_8.502805"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1447..1456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1834..1843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          774 a          862 c          793 g          814 t
ORIGIN      
        1 gtgcgatcgg ataagttgcc gcataagcgc ttgcaacgtt cggtgtgccg ctggtgttga
       61 tgagcgcacc cagcgccggt gtgctggtca tgccgccggt gatggaaccc aggttgttga
      121 acaggctcag cttcagcacc ttcttcgcca ggaagaaacc aatgaacagc ggaacaatgg
      181 tcatcagtgc gccccacagg aacagcagcg gaccatactt ttccagtgtc tgtacgaatc
      241 cggcaccgcc gtcaacgccg gcaccgatga ggaacagaac cagtccccat tcctggaaaa
      301 cctccagcag gtgcttgttg accttcatgc tcagtttgcc gattctgccg aagtgaccaa
      361 agaccagacc cataatcagc gggccaccag tcgttcccag gttgaaagag cctgcaccca
      421 gcggaatgtt gatgcagccc aggatggcac ctgtcagcac ggtcagcgca aatgcaccca
      481 gaccgaactt atccatctca aacagctttt tctgtttcag cggactttcc tcgccggtgt
      541 ctacggatgc cagctttgca cgttcttccg ccatgttggc tttcagcacc ttcgggatga
      601 tctgtacaaa cagaacaacg ccgataacgc cgaacggata tgctaccgca tgaccgactg
      661 cgatttcctt gaataccggg ctgctttcgc cgatcgcctc ctgtgcagcg gcaaatgccg
      721 gggtactggt cagcgcaccg gacagcagac caacgctcat tgcggagttc atgcccggaa
      781 ccagcagggt aatcagcgca cagacgcctg cgccggcagc gataatcact gcacccagca
      841 gaacatagga cttagcgttc tttttcagat tcttaaagaa ggtaggacct gcaatcagac
      901 caacggaggt cacgaacagg atcagaccga aactctggat aagcttgaag tagaagttga
      961 cagcgtcctg cgcctcaaca tcagcaatcc aggcagcgat gccgttctcg ccgaacgcca
     1021 cgcccttgaa atgtcccaca accagcgcaa ccaggaaaat cgcagcggtt cccagagaaa
     1081 cgcccttaat gttgatcttg cccagcagat agcctacaac gatgatcgca aagacaaaga
     1141 acagcaccat gacgatagca gacatactga aaatgtgggt gcctgccacc tcgaacagtt
     1201 ttagattcat gaatatacct ctccttgtga tgtttcgtgg aagttgccgg cggctctccg
     1261 gtcggacttc cagaaaaaac atttgtacac cggacggaaa catccagcgt acagcatacc
     1321 ttattattat aactgatttc atcttttttg tcaatattgc tgattgatat tttgaaaaat
     1381 atacgttttg tatgaatttc cacaggaaaa gtgtcgatta ggtgaaatgt ggtacaaaat
     1441 cagaggnnnn nnnnnnaagt ttctttgcct gcggcggcgg atttgacaga gggtgagaaa
     1501 attgaatcga atataggata tagaaaaatc gcctgtaact taacgtcaca ggcggttttc
     1561 tacggcatta ccgcacggag ccatacagct ttgggatttg tactgccatt gtttttgctc
     1621 cgggttctaa agaactaaga aagcgttcag tattttccac catgatgatt tcacctcttt
     1681 tttcacagac tctctcctat gttaccacaa ttttttccga aagtcaatag actacgaatt
     1741 taatcatttc aacacattga attacttgat aagagaggaa gagatggtgt ataataaaat
     1801 gtcatatctt cgtgaaacaa agatatgtgg attnnnnnnn nnnggattga aaaaatgtct
     1861 tgaatttatt gaatgttgaa agacaaggaa aaccgcctgt gatgttgtgt cacgggcggt
     1921 ttttctatct ctttcagtaa agtttatgcc tcttccagca tctcctccaa caactccgcc
     1981 gctgtctcca caaaccgcac gcacggccgc actttatagt attcctccgt gcgtggctgc
     2041 ggatcctgtt tcagcttgcc gggcagcgcc tgcagcagct cccgacagac cagggtatcg
     2101 tgtttctgcc ggaactgttc cgccagttcc cggatgcggg catagtgggc tttcttgccg
     2161 tcgtagtcag ccggatcatc gtatcccttt aacattcctg ccaccagaaa cattccggtc
     2221 acagcgccgc aggtctcccg cataccgccc atgccgccgc cgaaagacga tgccagccgc
     2281 acggcatcct ccagagaaag ccccatttcc tcgtgaaatg cgcccaccac agactgcgca
     2341 caattgtatc ctgcacggaa gagatcccgt gccttttctg catgattcat gcaatacact
     2401 tcctttttgc aaaaatgcca gtatgcgatc ataccggcat ttgttatcat aacatagaaa
     2461 tcgggatttg tcaatttttc gcagtctctg ctgtttcagc ctcagcctgt gccagcgcct
     2521 ggtacgcctg ttcgtagaag tattcctggg agttgacaag cggttccttt gccggctctc
     2581 tggtgtatgt gctgtccggc tgctgtacac gtgctttgac attgtccgtc atcatcacat
     2641 cgaacatatc caggatgcgg tgcttgatgc tgtcgtcata gatgggcgca gcgacttcca
     2701 cacgccgtct ggtgtttcgg gtcatccagt ccgcagaggc aatgtatacc ttgcagttgt
     2761 cgtgcgcacc gaagatgtag atgcgtgtat gctccagaaa acgtcccaca atgctgcgga
     2821 tagagatatt ctccgtcagc tccggaacgc cggcaaccag gcagctgatg ccacggacga
     2881 tcatttcgat ctttacgccg gctttggatg cctcgatcag tttttcaatc agatacttgt
     2941 ctgtgagcga gttgattttg atgccgatat aggcgtgttc gcctgccttt gccagggcgg
     3001 tctgttcgtc gatcatgtcc gccagcttgt tctgcaggca ctttggcgca accagcagat
     3061 gctccgtgtg ttccacggtc tgtcccaggc agagcgcatg aaagacccgg tttgcctcca
     3121 gaccgatgtc atggtttgcc gtcatcagcg aatagtcggt gtagatggca gaggtctttt
     3181 cgttgtagtt gcctgtgccg acctgggtga tataggagat ctctgtgccg acctttttgg
     3241 tgatgagaca gagcttgctg tgt
//
LOCUS       DAWENO010000207         3242 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_9994_length_3242_cov_8.393160, whole genome shotgun sequence.
ACCESSION   DAWENO010000207 DAWENO010000000
VERSION     DAWENO010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9994_length_3242_cov_8.393160"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    747..756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          662 a          926 c          823 g          821 t
ORIGIN      
        1 tccgtgaggc aaactatcct cgttgccgtc aaatttgcct ttccaggtga ttttcatgtg
       61 tacatttcct ccatattagt tataattccg gaactgctcc ggcactgccg tttcaaacag
      121 ttcccgaatc ttctccatgc tccacacctg gtaagacgga ccggtgctgg acgagcagta
      181 cggcagtcca tgctgcgacg ccagttcatc cgtatacata ctgtagggat acagccgcag
      241 gttggattct ctgccgtcgt caaactgggt gatgaccggc gacaccttga catcttcaat
      301 ggtgactgaa ccgtctgcct tcgccacaat gttaaagtct gccagttctc cgatcagatt
      361 catgttgtcc gtctgggcag aaatgaaatt tcccagcgag tagaacacaa agccctgggt
      421 gccgtccgga cgggtcagat attccatggt ctgtcccacg tgcgaatgcg tcccgataat
      481 cacatctccg ccccagtcga tgatcttctg cgccagcgct ttgacgccgt ctgtcacatt
      541 aaacgtatcc tctgcacccc agtgacaggt aacgaccacc acatctgcga tttctttggc
      601 tgcggcaatc tgctgctgaa tcaagtcctc ctgggaagtg tagataattt ccagttccga
      661 gttgctcggc agcgaatagc cgttggtgtt ctcggtatag ctgaggaatg ccactgtttt
      721 cccgttgaat tccttggtac ggatgtnnnn nnnnnngcag gtcgggatac tgctgcattt
      781 tctgatccca gtagttgaga cagctgttca agccgctttc gcccttgtcc agcaggtggt
      841 tattgcacat ggtgatgacg ttgaacccca tgccgaccac agcgtcaccc agttctgtag
      901 gcgagttgaa gttgaagttt gagccagaaa cttcgaaacc atctccgcag atcagcgttt
      961 cctggttgat gatattcaga tcgccgttga taagcggctt gatattgtcg tagcagtaat
     1021 cgaaattgta gcttgttccg tcctgggaat gcgcctgggc gctctgatag acgcactgct
     1081 gcaccaggtt gtctcccacg gcgaccacgt gaatggaagt gtctccattg gcatctgtgt
     1141 tcgaatccgc ctcattgtct gtgctgctgt ccgccggtgc atctgtgccg tctgctgtgc
     1201 cgctgtctga aaccggtgca ttcgccggaa cggctgcgct gtcattgatc tggctggtct
     1261 tgccgcaccc ggatacggca ctgaacagtg ccagcccaat cagaagtgcg gttgtccttt
     1321 tcccagacaa aaaaatctgt gttttcatga aaacgtcctt tcttcgacat aatctctcgt
     1381 ctattatacc acatctccaa taaaaaagca atctgttttg catctttttt tgcaaaggcg
     1441 gcatactgaa aagagaacaa gccctgtaca aggaggaaat ctcatggaac aaaaccaaac
     1501 ggaacagctg ctgcgtcagc ccattgcccc aaagttaacc cagaacttgg aacagatacg
     1561 caccattgcc gaagggtcat ccgacctgct ggtcaacgag tttgtcctca gcggactgcc
     1621 ggcggcactg ctctgctgtg agggaatgct ggcgacttcc atcatcaccg agctgatcct
     1681 caaccccatc acgaaaatcg ctctggaatg ccctactgcc gcaaatgtct ttgcctacat
     1741 tcaaaatcac cagctctatt ccaccgaccg ggcagaggca aaaacatatg ccgacctctt
     1801 ccggtttctc aactccggtt tcgccgttct ggtgctggag ggcgcatctt ctgcgctggt
     1861 attcgggata cagggctatg acaagcgttc tgtcagcgag ccgtccggcg aggacaatgt
     1921 aatgggcgca aaggacggct ttattgaggt tgtccgggtg aatatgtccc tcctgcgccg
     1981 acggatgaaa acgcctcagc tcaagctgga actgttcgtc aaaggtacga agagccagac
     2041 cgacctttgt ctctgctata tggcagaccg tgtgccgaaa cagctgattc gggaaatcaa
     2101 ggtacggctg gatgagatgg aactggaatc catcctttct tccggctacc tccaaccgtt
     2161 tctggaaaac cgccgcagct gcctttttga cacggtagga acgacccagc gcccggacgt
     2221 gctttgtgcc aagctgctgg aaggcagagt cggactgctc attgaaggca cgccctttgc
     2281 cctggtaatt cccaagctgt tctgtgaaag ctttcagacc atggacgact actgctaccg
     2341 cccggcatat gccacgttgg tgcgctggat caaatacgcc gcctttctgg tggctattct
     2401 gctgccggcg ctgtacgttg ccattgccct gcaccatccg gaactgctga accgtaccct
     2461 gctgctgatc ctcgccgagg cagaagaaaa cgcacctatt tcactgaccg ccgaagcaat
     2521 cggtgtgctg ctggtctacg agatcatacg agaggcaggg ctgcgtctgc caaaggcggt
     2581 aggcggtgct gtcagtattg ttgccgggct gattatcggg gatgccgccg tctcctccgg
     2641 actcatcagc acgcccatgc tcaccgtcac tgccatcgcc gtcattgccg gttttgtcgt
     2701 gccggatctg gcacagagca ttactctgct ccggctggcg tttctgctga ccgccggcgt
     2761 gctgggattg ttcggcatca gccttttggg cgctgtggtg cttttcaatc tctgtgccgg
     2821 cgaaagcttt ggatttccca tcactgcgcc ctttgcgccg ctgtaccgca agggattccg
     2881 ggacacgctg acacgagtca gctttcggaa gatgcagtcc ggcaatttca ctgtggagga
     2941 atattatgaa tagactccgt tccaatcaaa tctttgccat cctgctggtg agtgccgcct
     3001 ttcccctgct ttgccgcaca gacgctttca ccatcgaggg cattttcggc gcagctattg
     3061 cggcggtggt gcagctgctg ctttgcctgc ctatgctgcg gctgtatcac accagctttt
     3121 ccttttgcga ttacgcccga aagcaccgca tcctgcccct tttctttctg gcgtatctgc
     3181 tcatacaagg cggcatctcc tttgtccgcc tccagtccac gtcacaggac attgcgctgc
     3241 cc
//
LOCUS       DAWENO010000208         3223 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10061_length_3223_cov_12.340593, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000208 DAWENO010000000
VERSION     DAWENO010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10061_length_3223_cov_12.340593"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          706 a          916 c          900 g          701 t
ORIGIN      
        1 tcctcgcacc gtgcgcccat ctggatcatc tctgtattca gctttgccag ctgcgattcg
       61 taatactctc tcattatccg aacctccccg aaatgtagtc ctctgtccgc ttatcctgcg
      121 gattggagaa cagcttttct gtttctgcat attccaccac ttcacccagc aggaaaaagg
      181 cggtgcggtc agacacacgg gtcgcctgct gcatattgtg ggtgacgatg ataatggtat
      241 agtcattttt cagcgacatg accagatctt ccacctttga ggtcgagatc gggtcgagag
      301 cgctggtggg ttcgtccatc agcagtactt cgggttgcac cgccagcgca cgggcgatac
      361 acagacgctg ctgctgtccg ccggacatac ccagggcgct cttgttgaga cggtccttca
      421 cctcgtccca gatggcagcg tcccggagcg agctttccac gatctcgtcc agctttgcct
      481 tggaacggat gccgtgggta cggggaccgt aggcgatgtt gtcatagatg ctcatgggga
      541 acgggttcgc tttctggaac accatgccca cccgcttgcg cagcaggttc acatccatat
      601 cgccgtaaat gtcctccccg tccaggcgga tatcgccggt gatcttacag ccctctacca
      661 gatcgttcat ccggttcagg gatttcagca gcgtggactt gccgcagccg gacggcccga
      721 taaaggcgct cacctgatgc tccggcagtt ccagattgat atttttcagc gcatggaacg
      781 tgttgtagta caagtccatg tcatggatcg caaattttgc catagtcgtt ttctcctcat
      841 gggatatgga ttgggcgaat gatgatgtat tgtgaaacgg ctttataggg gcgaacattg
      901 ttcgcccgtt ggtgtgttac tcctccggca cttcgtgcca cctcctctaa agaggaggct
      961 tttacaaatc gtctcctctt tagaggagat gcccgacagg gcagaggagt caacccggac
     1021 gaacgatgtt cgcccctaca ttggaatggg tgacttacgc aatttcgtgc gctctctgct
     1081 tggcaatgcg ttaaaatccc tccggcactt cgtgccacct cccttttcaa gggaggcaaa
     1141 ttatttctca ccatgtcccc cttgaaaagg gggatgtcag cgtaagctga cagggggatt
     1201 tactcctcag tcaagcctac ggcttgccag ctcctctatg agaggagcct atatatgcct
     1261 cctctcatag aggaggtgtc cgcaggacgg aggagtcaac ccagtgcaac gcacccctca
     1321 cccactcact ttttcgtcag tttctttgcc gcatagccgg acagggcgtt gatgagaatg
     1381 accatcacca gcagcaccac cgcagtggca taggctgcgc ccatatactt gccctcgcac
     1441 agcagctggt acatatgcac cgccagcgtg cggcagggtt tcagaaagcc ggtgcagacg
     1501 tcggtggatg tgccggcggt gtacatcagc gccgcagtct cgcccacgat acgcccgatg
     1561 gcgaggatca cgccagccag aatgcccggc acagcgctgg ggagaatgat gcggaagatg
     1621 gtacgcagcc gtccggcgcc caatccgaaa ctgccctcac ggaaagaatc cggcacgctc
     1681 agcagcgcct cttcggtggt gcgcataatc agcggcagca ccatcagcgc catggtgatc
     1741 gcaccgccca gcagcgaata gctgaaccgg cacgccacgg cgaacgccag gaatccgaac
     1801 agaccgaaca caatggacgg gatgccctgg agcgtttcgg tagtcatgcg gatgatcttc
     1861 accagcgtca tctggaactt gctgcctcgg ttggaatatt ccaccagata gatggcggag
     1921 aacacgccga tggggacggc gatcagcagc gtcagcgctg tcatgatcag tgtattgatg
     1981 atagacggca tcatagaaca gttgtctgtg gtatacttcc aggcgaacat ggacgggcgg
     2041 agattcggca cgcccatcac cacgatgtac acgatcagac tcagcagcac gcccacggtg
     2101 atgactgccg ccagcagggt gacgatcagc acagccagcg agccgggatg ccgcttatag
     2161 gatgccattt tctgtcggaa ggtctgatgg ttgatgccaa cgatcttctc ggtttttccc
     2221 tctttcattt tgtctccttc tttttcacca gcgaaaagca aacgttgatc agcaggatga
     2281 agatgaacag gacaacgcct gtgccgatca gtgcgccccg gtgcaggtca gtggcatatc
     2341 ccatctccat gacgatattc gccgtcatgg tgcggacacc ggacagcagg ctcttcggga
     2401 gaacggtctg gttgccggcg atcatgacca ccgccatggt ctcgccaatg gctctgccga
     2461 tgcccaggat gatgcctgcc ataatgccgg acttcgccgc cgggagcgtt gcgcagtaga
     2521 cgctgcgctc atgggtcgca cccagcgcca gcgagccctc gtagtagctg tcgggaacgg
     2581 cacggatcga ggattccgag atctcgatga tggtgggcag gatcataatg cccagcagga
     2641 tgccggcggt gaggacgctt tttcctaccc cgaaaatgct ctgcatgatg ggaacgagaa
     2701 ccaccagccc aaagaagccg tacacaatgg acgggatgcc agccagcagc gagatcgccg
     2761 gctttaagat cttatacaat tttccggggc agaaccgtgc cataaagacg gaacacaaca
     2821 gcccgatggg aacgcccacc agaatggcga tgcccgtggc gtagatgctg cccacgatca
     2881 tgggcaggat gccgaactcg ccctcctggg gtttccagac cagaccggtc agaaacttca
     2941 gcggtccgat ctccgcaatg gcaggaaagc cgttcacgaa caggaatccg cagatcagca
     3001 ccaccgccag gatggacaca caggctgcca ccagaaagac cactttcatg atggactcac
     3061 gatatttttt catggatgtt attctctcct ctctgagagg gtggtttcaa ccgttttttg
     3121 agggtatggg tggaaattgt ggaaatatgg gtgcgggatg tcttatcatg cctcccttga
     3181 aaagggtgat tccccacggg gtggggaaat gtcaccgtag gtg
//
LOCUS       DAWENO010000209         3133 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10356_length_3133_cov_11.059779, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000209 DAWENO010000000
VERSION     DAWENO010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10356_length_3133_cov_11.059779"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          761 a          764 c          957 g          651 t
ORIGIN      
        1 acccaggtgc agatcgaagc cattgaggca taaatgattt ccgcacgttt ttttcttgca
       61 ggagagcagt ataccggctg tgagatcacc ggacacgccg attattcgga agaggggaca
      121 gatgtgatct gtgcctatgt gtcggctgcg gtgcagaccg tggcaaatct catgacggag
      181 atcttccggc ttcccgtttc ggcggaggag accgaagaac cggcgagggt atcgctccgg
      241 ctttcggaaa aagatgattc cggcaacgcc caccgtttat ttgcggggct ggcgttacag
      301 ctgggagagc tgtcgaaagc gtttcctgcg aatattcaag tcacacacat ggaggtgtaa
      361 gtcatgctga agattaatgt acagtttttt gcacataaga agggttctgg ttctaccaag
      421 aacggccgtg attctgagtc caagcgtctg ggcgtaaagc gtgcagacgg tcagttcgtt
      481 aaggctggca acatcctggt tcgccagaga ggtactcaca ttcatccggg cgcaggcgta
      541 ggcatcggtt ctgatgatac cctgtttgct accgttgatg gtactgtaaa gtttgagcgt
      601 gttggccgtg accgcaagaa ggtcagcgta tacgctgcac agtaacgaaa acgacataat
      661 ttgtcggatt gcgtgaagaa cgacaggaaa tcccgagcag cgtttgtgtg cttgggattt
      721 tcttattata cttcacgtga ttgcaaaaga tgcagggcag aaatgtgtct tttgcaatcg
      781 tgtgaagtat agctgtgacg tgtgaaagat acaccgtgaa atgagaggag atgtgagaga
      841 tgtttgtcga tcaggcacga atttcgatca aagccggcga cggcggcgat ggagcggttt
      901 cattccatcg ggaaaagtat gtggcggcag gcggcccgga cggcggcgac ggcggaaaag
      961 gcggcgatgt ggtctttcag gtggatgata acttttccac attgatcgac ttccgctata
     1021 agcgaaagta caccgcccag cggggcgaaa acggcggtgc gagaaactgc accggaaaga
     1081 gcgcaccgga tctggtgatc cgtgtgccac gtggtacggt cgtccgggag acaaagaccg
     1141 gccggatcat ggcagacctc tccacggacg agccgcagat cctggcaaag ggcggcagag
     1201 gcggcaaggg caatatccac ttcgccacct ctacccggca gattcctcgt ttcgcaaagc
     1261 cgggctttcc cggtgaggcg ttcgatgtca cactggagct gaaactgctg gcagacgtgg
     1321 gactggtagg attccccaac gtgggaaaat ccacgctgat ctccgtggtc agcgccgcaa
     1381 agcccaagat cgccaactat cacttcacca ccttgacccc ggtgctgggc atggtgcgtg
     1441 tggcagagga acagtccttt gtcatggcag atatccccgg actgatcgag ggcgcaagtg
     1501 acggcgtggg actgggtcac gacttcctgc gtcatgtgga gcgctgccgt ctgattctcc
     1561 acgtggtgga cgtttccggc agtgagggca gagaccccat cgaggattat gataccatcc
     1621 agggcgagct ggagcgtttc cgggaagatc tggcagagcg gccgcagatc gtggtcgcca
     1681 ataagtgtga tatggctgac ccggaacaga tcgcacggtt ccggcagtat atcgaagaaa
     1741 agggactgcc gttctatgag atctccgcag ctaccaccca gggaacggcg gaactggtac
     1801 aggcgaccgc agccatgtta cagaccctgc cgccgattct ccagtatgag gcagaggcgc
     1861 cttcgccgga ggaactggca gaaaatgccc acggcggttt cgagatcgaa gtagaggacg
     1921 gcgtgtatta tgtcaacgcc atgtggctgg agccgattct ccgcacagtc aatatggagg
     1981 actattcctc gctgcagtac ttccagcgtg ttctgcgcag cagcggcatc attgatgcac
     2041 tggaagaaaa gggcatccag gagggcgaca ccgtcgatat cctgggcttt gaatttgact
     2101 tcgtgccgta atgaggtgag ggtatgaaac cgttgacaca agcagaggcg agacagtata
     2161 gtcccatggc actggcattt ctgggcgaca gcgtctatga agtgatggtg cgggaatatc
     2221 tccttcgtgc ggcaaatcag ccggcggcaa agctgcatga ccaaaaggta caactggtct
     2281 gtgcaggctt tcaagcggcg gcggcagacc gtctgctgcc cgttctgacc gagaacgaga
     2341 ccggcgtcta taaacgtggc agaaacgcca acaataccgt tccacgccat acctccgcac
     2401 aggattatca taaggcgacc gggctggaaa gcctgttcgg ctatcttcat ctgatgggtg
     2461 cgcaggagcg gctggaagaa ttatttcaaa ttgtgatgaa tgcagacggt gcgtctgcgg
     2521 aagaataagg agtgtttacc aatgtcagga cattcgaagt ggaataatat caagcggaaa
     2581 aaggaagcaa ccgatgccat caagggcaag atcttcacca agatcggcag agagatcacc
     2641 gtttgcgtca aggaaggcgg tcccgacccc aacagcaacg ccaagctgcg tgatctgatc
     2701 gcaaaggcaa agtccaacaa cgtgccgaac gacaacatcg accgtgtcat caagaaggct
     2761 gctggcgagg gtgacaagaa caactacgaa tccatcgtat acgagggcta tggtcccaac
     2821 ggcgtggcgg ttatcgtaga gtgtctgacc gacaacaaga accgtactgc aggaaatgta
     2881 cgccactatt tcgacaagtt cggcggcaat ctgggaacat ccggctgtgt atcctttatg
     2941 ttcagtgaaa aaggcgtgct ggtgctggaa aatgacggtc tggacgagga ccaggtcatg
     3001 gaggactgct ttgagttcga tgcagacgat ttctccatgg atgacgaaga ggtcatcgag
     3061 gtctcctgcg ctccggcagc cctgcgtggt ctgcgtgagg ggctgaccga aaagggctat
     3121 cacgtgctgt ccg
//
LOCUS       DAWENO010000210         3130 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10373_length_3130_cov_7.946992, whole genome shotgun sequence.
ACCESSION   DAWENO010000210 DAWENO010000000
VERSION     DAWENO010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10373_length_3130_cov_7.946992"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          651 a          817 c          838 g          824 t
ORIGIN      
        1 agccgtagtt ggacttggtg atgacgccga cctcagagat gccggagttt accatgttgg
       61 aaagcggaaa gtctaccatg cggtaacgtc cgccgtaagg gatcgaaccc atagtccgca
      121 gcttggtcag atcaataact gcggagtcgt tgatgcttgc aaaaattaaa cctaaaacat
      181 tttgattgat actcattgta cgccaccgcc tttacagatt ttcggaaata atttctttcg
      241 gaagaactcg cttgccggaa gagattgtga tattatgacc gagaacggcg atgccccaat
      301 cctcacggtt gggaacagat tccggattca taccgatcat ggcatcggat tctaccacgc
      361 agttttcacc gatgatcgca tattccacca ctgcattctt cttgatggtc gtgccgggca
      421 ttacgatgct gtacttgacc gatgcgccct cttcgatggt gacaccggag gagatgatgg
      481 agaagtccac cgtaccgtct accacgctgc cctctgtaat catggagttt tctacgtgtg
      541 catttgcgcc gatgtactgc ggcggcatat tgttgtgacg ggaatatacc ttccagtccg
      601 gatcgtacag gttcagcggc acattcgggc tgagcaggtc catgtttgcc tcccacaggc
      661 tgtccagtgt gccgacatcc ttccagtagc cctcgaattc ataggcgaac aggcgctcat
      721 cattttccag cattgccggg atgatgtcct tgccgaagtc cttcgaaccg gtattggcgt
      781 tttcattttc gatcagatat ttgcgcagct tttcccatgt gaatacgtag atgcccatgg
      841 atgccagtgt ggatttcggc tccttcggct tttcggtgaa gtctacgatt ctgccgcttt
      901 cgtcacaggt catgataccg aaacgggatg cctccgaacg gggaacatca attacggcga
      961 tggtgctgtc agcctggttg gcaatgtgat aatccagcat cttggaatag tccatcttgt
     1021 agatgtggtc accgcccagg acgatgacat attccgggtc atagcgctcg atgaacgcca
     1081 tgttctgata gatcgcattg gcagtaccct cataccagga tgcgcctgct gcggtctgat
     1141 acggcggcag aacgtgtaca ccgccgtgca tcttatccag atcccacggc tgaccgttgc
     1201 cgatgtattc gttcagcacc atcggctgat actgggtcag aacgcccacg gtatcaatac
     1261 cggagttgat gcagttggag agcggaaagt cgatgaaacg attcttaccg ccatagggga
     1321 tcgctggttt tgccactttt tgtgtcaggg catacagtcg gctgccctgt ccgccagcca
     1381 acagcatggc gacgcacttt ttcttgatct tcatgatatt cctcctaata taaattcaaa
     1441 aatttctttt gaatgggtga cataatggaa aagctgtccg gctcacctgt tgctgccggg
     1501 agccgctttg cgctttctcg cagtgcctgc cgtttttttg acggtcgtct ttttcggctt
     1561 gacggctgcg ctctttgcag gggatgcctt tgccgctgcg gactttctcg gcgcacgctt
     1621 tttcaccgga ctgtacttca gatagagtac agaaagcggt gcaagggtga gggagatgca
     1681 ctgttcaaaa ccgtgcatgg caatggggag cgtctggaat ttcttttccg tgacgccgct
     1741 gccgccatag actgctgcat ctgtattgaa taccacctgg tatctgcctt ccttcggcac
     1801 accgatctta taatcgttgc gctgcaccgg gcagaagttg cagatggcga tgatctcgtt
     1861 tccgtcgtcg tcaatacgcc ggaaggcgat gacgctctgc tgatagtcgt cgtgggagat
     1921 ccagctgaat ccctgccagg aatcgtcatc ctgccagagt gcggcgttgt ccagatagaa
     1981 tttgttcaga tcctgtgaga actgttccat ctgcttgtgt gccggctgtt ccaggagttc
     2041 ccagtccagc tgcgtctcat agttccactc ttttttctgt gcgaattcct gtcccataaa
     2101 gagcagtttc tttccgggat gcgccatcat atagccaagg aatgcccggt aagaggcgaa
     2161 cttttcttct tcggtctgac cgctcatctt ctccagcatg gagcattttc cgtagacgat
     2221 ctcgtcatgc gaaatgggga gaatgtagtt ctccgagaaa cagtagaaga acgagaacgt
     2281 cagcttgtcg tggttgaacg cccggtagat ggggtcgagg gagatgtagc tgagcatatc
     2341 gttcatccag cccatgttcc acttgaagtt gaatcccaga ccgccgatgt cggtgggttt
     2401 ggtgaccatg ggccatgctg tggattcctc tgcgatcatg aggatgtccg gattccggga
     2461 gaatgccgcc tcattcagat gtcgcaggaa gtctaccgcc tccaggttct cgtgaccgcc
     2521 gtaaacgttg ggacgccatt cgccgtcctg ccggtcgtag tccagataga gcatggatgc
     2581 cactgcgtcc acccggagtc cgtccagatg gaagtcctcc agccagcagc aggcgctgga
     2641 gatcaggaac gagcatacct cgcctttgct gtagtcaaat actctggttc cccagccctt
     2701 gtgttccatt ttcagcggat cggcatattc atagcagtat gtgccatcga attcgcacag
     2761 ccccatgggg tctttcggga agtgtgccgg aacccagtcc aggatgacgc cgatgcctgc
     2821 ctggtggaac aggtcgatca ttttccggaa gtcgtccggc gtgccgtgcc gtgaggtggg
     2881 cgcaaagtaa ccggtgacct ggtatcccca tgagccgtca aagggatact ccgtcagcgg
     2941 catgaattcc acgtgggtat aggacatctt tttcagatag gggatgatct cttctgcaaa
     3001 gccgatataa ctggggaact tctccgccgg gttgtgcttc caggaggaga tgttcacctc
     3061 atagatgttc atggggcttt tgtaggggac gtgctttttc ttttccagca tccattcccg
     3121 gtctccccat
//
LOCUS       DAWENO010000211         3128 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10377_length_3128_cov_12.491377, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000211 DAWENO010000000
VERSION     DAWENO010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10377_length_3128_cov_12.491377"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2412..2421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          740 a          932 c          643 g          803 t
ORIGIN      
        1 tgtcgatctc ccgatccgct cttccacttt ctttcattca tttgtcctct tgactaccgc
       61 tcccgtgcga cagctttatt attataacac gctcgctcga ctttgtcaag cataagtttg
      121 tgaacattaa aatctggata ttgtacaaga tatacaatat ccagattctg tcagcctatt
      181 tccttcaaat ctgcacttat cccgtctcag ttatcttcta tttcctctgt ctcctgctcc
      241 ggcaaaatct ctgccttgat ccgtgagatc cagttttcct ccatcgtcag caccgtaaac
      301 cgaatattct tccactgcac cgttggattc tcatgcgcct ccggaatata ccccagcaaa
      361 tccactacaa agccgctcat ggtgtcaaac gccatatcct ccggcaactc atgtcccagc
      421 aaaggcagcg tctcttccgg atcagcgtgt ccattgatta gatacagcga atccgtcaca
      481 cgatgcacct ctgcctcttc ttcatcatac tcatcctgaa tattccccac aatttcttcc
      541 agaatgtcct ccatgcagac cacgccggca gtaccgccgt actcgtccac cacaatcgcc
      601 atctgtgcct ttttccggga catctccgac aatacgtcat cgcactttgc cgtttccggc
      661 acaaacagcg cctcacgtag aaatcgtttt actgaataac cggaaatatc gggacatccg
      721 atcagttcca gcaaatcctt ggcattcagc acgccaataa tgttatcaat attatcctga
      781 tacaccggca tccgggaata tttctcctca attgccagct gcaccacgtc agagattgcc
      841 tgttccacgt ccactgccac cagttctttc cggtgcgtca tgacatctga aatagggata
      901 tcatcaaact cgaaaatatt gtttatcatt tcacgctgct gctcttcgat cacgccgttt
      961 tcattgccag cattgaccag atgcaggata tcctcttcgg tcacgtgttc ctttgccgga
     1021 cgctttcctc ctgccaggca gtccatgccc tcatagattc catagaccgg cagcaaaaga
     1081 agtgcctcca gtttcaccaa tcccagagat ttttccgcca tttgttccgc ctgttttctg
     1141 cccagtcctc tggcgcagcc gtccaggatc agtacaagcg ccaatgcagc cagcgccgga
     1201 acgaccagcg tcacccacgg attccaccga tgcgcccagc ctttttcaaa taccagcaaa
     1261 tacgccgcca gcgccaggat tgccgtaaac gagatcacct ccgccactgc cgtaatgcgc
     1321 agccgattct tgtgtttcag ataatactgc attttcggct tgtcctctgc aagagcgctc
     1381 atctgaccgc ccagtttctg taccgacgcc atcgccgccg agaaccacag atgcagtccg
     1441 cagagcagca ccgccgccag aatcagcaca gtaccgaccg ccgaatggaa cgaatcataa
     1501 tccataatct actcctattg ttatgacagc gccgtagaaa gcccagtgct gccttatatt
     1561 cttatataaa aaaacgccgc cagatctatt gcactggcag cgcttctgtt tgtatcacga
     1621 atttaccgtt cttcacagat caatttgatt gcagtgcggt cttctccgtc aatctggata
     1681 ttggcaaatg ccgggatgca gatcagatcc acaccgatcg gggcgagata gccacgggca
     1741 attgcgattg ctttcacggc ctgatttgct gcgccagcac cgacagcctg tacctcaacc
     1801 atcttttttt cacgaatcac accagcgatt gcgccagcaa cagaattcgg agaagagtgc
     1861 gatgatactt tcaatacttc catgataaaa gcctcccata gtacaaccat ttgtgatata
     1921 ctgcatccga tcattctgtc cgccggtttc ccggggcaga aaaatgcgtg taatttcccg
     1981 gagcgttgcc gttccggatt ccggcacatg atttgctcac catcattata caacaaagcc
     2041 tgcgaaattg caagtccctg ccgaaaaatt tgtgcattat cgaacaatca ggcgctcaat
     2101 tttcgtacat ttgccacatt tttcatcaaa cgtaatgata acgccgttca aaaagcaggg
     2161 atcgctgccc tctgtgaacc gtgtaggaaa atggaaccgg aaccgatcca gcgccggctg
     2221 taccgcaacg cccaggatag attcctccgg tccggtcatg cccacatctg tgatatagcc
     2281 cgtatgttcc gacaaaatcg tttcgtctgc cgtctgcaca tgggtatgcg taccgactac
     2341 cgccgtcacc cgtccggcga ggaaatagcc catcgccttt ttctccgctg ttgcctcagc
     2401 gtgaaaatcc annnnnnnnn ncggcgtttc gatctcctcc agcagttctt ccaccacggt
     2461 gaacggattg tccagcggat ccagatacgc cgttccggac agattcagca ctgccacctg
     2521 gcacggacca aagtcaatgg tgcagatgcc atgtcccaca cagccctccg gataattcgc
     2581 cgggcgcagc accaccgggt tgtcatagat tgccagcgcc tcacgccgct taaagcagtg
     2641 gtttcccgtc gtaatcacat ctgccccggc agaaaagatc tgctccatgg agtgcttggt
     2701 gataccgttt ccctgggcgg agttttcccc gttgacaact gtcatatcaa ttttgtgcgt
     2761 tcgtttgatg ttcgggagac gttcctgtaa aaaacggctg cctgccgcac ccacaacatc
     2821 tccgatgtgt aatagtttca tgtatgattc ctttctaaga aaacgccgta caaagccgtt
     2881 agagcctgtt cagcggtttc atgagacctg ttccagtctg gcattgatta tatcacgttc
     2941 catgccgcag tgtcaagcgg tttctgtcga acgcgaacgg atttgaattt gcccgttttc
     3001 tgcgtccgca aaaaaaataa agcacacaag agcgttcctt gtgtgcttta caatggaaga
     3061 gataatacaa tgcaatggag cggattacga ggctcgaact cgctacctcc accttggcaa
     3121 ggtggcgc
//
LOCUS       DAWENO010000212         3078 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10542_length_3078_cov_10.548131, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000212 DAWENO010000000
VERSION     DAWENO010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10542_length_3078_cov_10.548131"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          710 a          814 c          916 g          638 t
ORIGIN      
        1 aaatagtcca ggggacagtt ctggtcgtag cggtctttca gatagatctt gctggcagcc
       61 acccggtcac agaacatctc cgccagaaag cgcaccggca tttccaccgg ctcgacccgt
      121 ttggttttcg ggttatagtc cgaccagtac tcgaagtggt ggcggttgcg ccccttgtgg
      181 tgcatccacg ccagagaata gccgtatgcc tcacgttcgg cttcgttggg gctgcgtttg
      241 ccgtcctcat aatagcggac accctgccag aactctgtgg ggctgtactt ggaaagatcg
      301 tggcgcagtc cctgccagaa gatccccgct ttgcggcagt gccggataac ggcgtgccgg
      361 tgccgggtga tggtggaaag atgtccccag aaagaatgcc agcccataac aagaacctcc
      421 tctgtgtgga atacctcttt cagtataccg cttttggaag gaaaagtcaa ggtttatgtg
      481 gaaagaagaa ttcacgcctc agtcagcctt acggctgcca gctcctctaa aagaggagcc
      541 tggaaaaaga gtctcctctt ttagaggtga ttccccacgg ggtggggaaa tgtcaccgta
      601 ggtgacaaaa gggaccgctc agtacgagat gcccgacagg gcagaggagt tgttatgcca
      661 ttttgagaag ggatacccgg aaacccacca acgggcaaac aagatatccc cctgtcagct
      721 tacgctgaca tccccctttt caagggggac aagatgagtg gaaataagcc tcccttgaaa
      781 agggaggtgg cacgaagtgc cggagggata gtttctatgt cggtgcaagg tatcccaatc
      841 cactgtaggg gcgaacatcg ttcgcccggg atacccggaa acacatcaac gggcaaacca
      901 tgttcgcccc tacaaaggcg ctgggaaatt atgcaagata atcccgacct ctgctcctca
      961 cggagcatcc acctcagcac aaggcacacc ctacgggcgc cttttcaagg gaggctcatt
     1021 tattgtcccc cttgaaaagg gggatgtccc gaagggacag ggggatataa agacgctgtc
     1081 aaacggaaat acacgagatt atcctgtaag atgttgtatt ttcccccgac accccttgac
     1141 ttttccggaa aacctggtag aatccccagg tgcggaaggg aatccaactt gacaggagag
     1201 atttccgatg aaagaaaaaa tctgtgccgg gctgggaatg ctcggcggta tgctggcggt
     1261 ggcactgggc ggctgggaca ccggtctccg ggtgctgctg tgtctgatgg cggcagattt
     1321 ctgcaccggg ctggtgaatg cggcagtgtt tcaccgttcg ccgaaaaccg gagacggcag
     1381 gctggacagc cggcgctgtt tccagggcgt gtgccgcaag gcggagatgc tggtatttgt
     1441 gggcgctgcc catgccatgg atctgctttt ggaaagcacc tttctgcgca gtgctgttct
     1501 gatcgggttc tgtgccagcg aggtcatttc gctgacggaa aacgccggct gtatggggct
     1561 gcccttgccg gcggcgtgga aaaacgccgt gagtctgctc aaccgcaaat ccgaaaccga
     1621 ctcgcaggaa aaggagtgac acgctatgca gatacaaaac gcatatctga cccacaaccg
     1681 tccccgaacc atccggaaac agaccactgc cattgcggta cactgggtgg cgaatcccgg
     1741 cagttccgcc atggcgaacc ggaactattt caacagcacc agccgctccg tgtccagcaa
     1801 ttacattgtg ggattgcagg gcgaggtgat ctgctgcatt ccggacgagg agatgagctg
     1861 gtgtaccaat gcagcgaaca gctacacggt ttccatcgag acctgtcacc ccgactggac
     1921 cggggcgttc ggcagcaaaa cctatgccag cctggtggaa ctgacggcgc agctgtgccg
     1981 gaagtacggg ctgcatcccc agcacggcgg cgtgatccgg cactatgacg tgacgggaaa
     2041 ggtctgcccc aagtggtttg tcccggcgag caaaggcggc tctgacacgg cggacggggc
     2101 gcactggcag cagttcctgc gggacgtggc gaacgctatg ggtaccggaa acgctgcggc
     2161 atctgcaccg acaacggcag cgtctgccac aggatatcgg gtaaaggtca cggcagatgc
     2221 cctgcacatc cgcagcggaa acggcacgtc ctatccggtg gttggttgca tccgggacaa
     2281 gggcgtctat accattgtgg agacaaaggg tgactggggc aggctgaaat ccggcgcagg
     2341 ctggatctgt ctgcggtatt gcaggaaggt gtgagagggt aaggaagatt cctctgttga
     2401 atttatccct ccggcacttc gtgccacctc agcacaaggc acaccctacg ggcgcctttt
     2461 caagggaggc tttttattat gtcccccttg aaaaggggga tgtcagcgta agctgacagg
     2521 gggatattca gaaatcaacc aacgggcaaa cagtgttcgc ccctacggtg aggtgggatg
     2581 ctatagggtt ttctgataca tgactcctca gtcagcctta cggctgccag ctcctctaaa
     2641 agaggagcct agaaaaaagt ctcctctcat agaggagatg cccgacaggg caggggagtt
     2701 gtcgtgtccc tttgaaaagg gatacctgga aacacaacaa cgggcgaaca gtgttcgccc
     2761 ctacggtggg gcatatgtcc cccttgaaaa ggcgcccgta gggtgtgcct tgtgctggga
     2821 tgtcccgaag ggacaggggg ataatccctc cgtctgctcc ttacggagca tccacctccc
     2881 ttttcaaggg aggcatattt atcgtcctcg gtgggatgcc ataaggtttt ccatttccgg
     2941 tacactcggg tcgatgtggg catcgacctc tacaggggaa cttcaaaggg caaaaaagat
     3001 gcctgaaaaa cgcaaaaaga gcttgtcggg aacggcttta tcccggcagg ctctttttgt
     3061 tatctggtgt tgtgatcc
//
LOCUS       DAWENO010000213         3077 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10547_length_3077_cov_7.401059, whole genome shotgun sequence.
ACCESSION   DAWENO010000213 DAWENO010000000
VERSION     DAWENO010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10547_length_3077_cov_7.401059"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    503..512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          891 a          678 c          720 g          778 t
ORIGIN      
        1 gactctgacg aaactggagg caatgacttc tgtatgctcc gttggtctgg atatgattgc
       61 tgttccaggc gatattgagg catctaccat ttctgctatc attgcagatg agatggcaat
      121 cggcatggtc aataataaga caaccgcagt tcgtctgatt cctgcaatcg gtctcaagga
      181 aggcgatatg ctggaatttg gcgggctgtt cggcagcgct ccggttatgc ccgtaaagcg
      241 caaatcggct gaaaagttta ttgctcgtgg cggcagaatt cctgcgccga tgcacagcat
      301 caaaaactaa gcgaaatatg cagacacaca caacagatct tatgataatc ggcggcggag
      361 cctcaggcct cgccgccgct gttgcagcaa agcaacttta cccggagatt tccgtcacaa
      421 ttctggaacg aaacgcacgg gtcggaaaaa agattcttac aactggaaat gggcgctgca
      481 atcttggaaa cactaatcaa gannnnnnnn nngatcgttc gcaaaacaat gtaaaatgat
      541 attctctgac acacaatcgg cagaatcttt ttttcagaat atgggattat tttcccggca
      601 tgaaaccgat ggcaggctct atccgctgtc caatcaagct gccagtgtat tggatgccat
      661 tcgctttgct gtgcagcagc ttagcgtttc tatcatctgc ggctgcgatg ttactacgat
      721 ccgaaaatca gaaaacactt tggaagcaga gacaacacaa ggcgtatatt ctgcatctgc
      781 ggttatttta gcagccggtg gatttgctgc accgaaaaca ggctctgacg ggtcggcttt
      841 ctcctggctg aaacaacttg gacatacaat tgtgccagtc aagccggctc ttgttccgtt
      901 ttatacagag ccaaaacttg ttcatccgct aaaaggaatt cgaatttctg cggaagtcag
      961 tgcagtttct gaaagcggaa cagttctggc aaaagacaca ggagaagtgc agtttacaga
     1021 acgcacgatc tccgggattt gcgtcatgaa tctatctgcc cgatgctgca aggaagaacc
     1081 ggcaatgctt tccctgcgcc ttcttcccaa tattgcgcct caaaaaattg agtcaatttt
     1141 atgggaactt tatggcattc gtgcaaactg gaaacaggaa gactggttat ccggactttt
     1201 tccgaaaaag gtcggcatac agttacttcg agccgcagga attactgcat ctttggaaaa
     1261 tcctgtttat cagattacac agtcacagct agaacaagtt gcagaaaaca ttctgtcttg
     1321 gaagtttcct gtgctttctc gtggcggatg gtccgagtcg caggttaccg caggcggcat
     1381 ttctgcgaac gaaacagatg atacactaca gtcaaaaatg atggaagggt tattttttgc
     1441 cggtgagatt ttagatcttc atggagactg cggcggctac aacttgaatt gggcatggca
     1501 gtcggggcag tttgccggaa aaaatgccgc tgaatatgtt atacagaaac gaggtgaaaa
     1561 ccattgatta agctaaccaa tttatctatg ccgctgaatt ttacgcaaga gcagttgaga
     1621 caaaagattg cggagcgtct aaaggtatca aaaaaggata ttcagtctat cacataccta
     1681 aaaaagtcaa ttgatgccag aaaaaagcct aaaatcacag tgattctaac cgttgcactg
     1741 caagttgcaa acgaagacaa gctgcaaaaa caaatcaaaa aggacaaaga tcttcaaatc
     1801 tatacgccct atcactatac cattccaaag tgcagcttat ccacgttaga acgacccgtt
     1861 gttgtcggat ttggtccggc aggaatgttt gccgcattgg ttttggcaag ggcaggatta
     1921 cgtccgatcg tcctggaaag gggttcttgc gtggaacagc gtcaaaaaaa cgttgccctg
     1981 tttcgtcaaa ccggaaaact ctctacagag agcaatattc aattcggaga aggcggcgca
     2041 ggcacatttt cagatgggaa actaacgacc ggcatcaagg attttcgaat tcgttatgtc
     2101 ctgcaaaatt ttgttgcatt tggcgctccg gaagaaattt tatatctggc aaaaccacac
     2161 attgggacag ataaactggt gcatattgtc cgtgaaatcc gaaaggaaat cataaaatac
     2221 ggcggcgaga ttcattttca agcccgaatg caaaaatttg cccagaaaga tcataagctc
     2281 actgccattt attatgaaaa ggatggagaa ctttttgaaa tttctacatc ccactgcatt
     2341 ctcgcagtag gtcacagtgc cagagatgtc tttgagatgc tgtacaacac aagcatttca
     2401 ctgatacaga aaaattttgc tgtcggaatg cgtattgaac atccgcaaaa ctggctgaat
     2461 tacgccatgt acggcaggga agtgctttct gacgaactcc cccaggcaga ttataagctc
     2521 gcagttcacc tttccaataa acatagtttg tacacatttt gtatgtgtcc aggtggtgag
     2581 gtggtagccg cagcctctga gcaaaatcga ctggtggtaa acggcatgag taactatgct
     2641 agagacggca aaaatgcaaa cagcgccttg cttgttggga tttcatccgc agaactgcaa
     2701 gattcacatc cattggcagg tatgtatttt cagagaaagc tggaagaatc tgcatttcaa
     2761 gccggcggcg gcaattacta tgcaccaact tgctgcgtcg gtgacttttt gcaaaaacat
     2821 tccgccacag gatgtggaaa cgtcacgcca acgtatcaac ctggtgtaac atggatctca
     2881 ccagatgaat atttaccaaa atttatcact gaaacactgc ggcttggaat cccagccatg
     2941 gactcgaaaa ttcatggctt tgcaatgtca gatgcagttt taaccggcat cgaaagtcgt
     3001 agttcatcgc cggtgcgtat tgtcagaaac gaaacctgtg agtcagtatc gctgaccgga
     3061 ctatatcctt gcggaga
//
LOCUS       DAWENO010000214         3063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10599_length_3063_cov_10.827128, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000214 DAWENO010000000
VERSION     DAWENO010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10599_length_3063_cov_10.827128"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          736 a          825 c          773 g          729 t
ORIGIN      
        1 agaattgctc cttccttccg gtatctgctg tgtgatacag tgaatattgc cgccgcccag
       61 cagaatttct ctggcggcaa tgggaatgat ctttcgtgtg gggaactgcg ccgccaggat
      121 ctcgcaggca gtcttgtcca tcgggtcgcc gaactgcggc acaagaacgc tgtcgttggc
      181 gatatagaaa tttacatagg atgccgccag acgctcatcc acgctccggg tcggctcgcc
      241 gtcccagaga tccagcgcat cgcattcctc ctgggtgacg cagaccggcg tctgcggaat
      301 gggcagctgt gtcacttcca gctttctgcc ctttgcgtcc gtctgctgtt ccagatagtc
      361 ggcacaggct ttggaaaggg catactgcgg gtcgttttcg ttgtccgtcc acgccagaac
      421 caccttgccg ggcgcagtga acgcacagac gttgtccacg tgttcgttgg tctcatctcc
      481 gtagatgcct ctgggcagcc acagcacctt ttccgcaccc agcatgacgc agagtttttc
      541 ggtgatctcc gcctttgtca tctgcggatt gcgtccggcg ctgcacaggc agctttccgt
      601 ggtgaggata gttccctcgc cgtcgctgtg gatgctgccg ccttccagga taaagtcccg
      661 gctgtcgtag gtgtccgtcc agaacaagtc gcagaccttc cgtgccacat ggttgtcctt
      721 gtcccacgga aaatacagcc cgtcctccag accgccccag gcgttgaaac cccagtcgat
      781 gccacgaatg cctccggcag cgctgcggac aaaggtggga cagacatctc tcgcccagga
      841 gtcgtccgtc tccatttcca ccagacggat ctccggcggc agcatggcac gtgccgtctc
      901 gtactgtgcc ggcgagacca gcatggtgac ggtctctgac tgtgcaatgg cagatgccac
      961 cgccacaaat gcctttcgtg ccggccagcc gccgctgcgc caagagtcgc cccgttcggg
     1021 ccagatgagc agacagcctg cgtgccgttc aaattccgcc ggcatggaaa aaccatccgt
     1081 ttttggtgtt gtatgtaaaa tgtgcatggg atcgctcctc tctgaattgc aaggaatatt
     1141 ataacatatt ttctttggga atgcaatcga attctatggg cggtttttca cgagattgtg
     1201 gaaatggtgt agatttcgtg gaaggcaggc tctgaatcct gtataaaatc ttttcggcgg
     1261 cacacaatac gttgggaggt gatgcaaaat gacaagcaaa gaattgctat acgtggagga
     1321 tgcactggga catgaaaagt attttcagac caagtgcaat gaggctgcgc agcagatcca
     1381 ggacgaaacg ctgaaacagt gcgtgatgca gatggcagaa aaacaccgcc agctgttccg
     1441 gacattctac ggactgctgt agcgtgtgtt cccataaaat ggaggaaaca aaaatggacg
     1501 acaaaaacgt aatggaaaac attctgctgc tggaaaaggg cgtgtgtgac ctgtatatgc
     1561 acggcaccat cgaatcctgc accagcaacg tacaccaggc gttcagccat gcgctgagcg
     1621 actctctcgc catgcaggat acgctgtatg accagatggc tgcaaagggc tggtatccca
     1681 ccgaccgtgc cgagatgaca aagatccagg cggtcaagaa aaagttccag tccatgcagg
     1741 gctgacccag acgcaaaaaa gctgccgtat ttctgcggca gcttttttca ttacggaatc
     1801 aaaaggttct cccatttcag cgaaaacacc ggcacgtcct gcatcccggc ataggagacc
     1861 atgctccggt ttgcacttct cgtcccgaat atcacaagca gatcactggc gctgcacatg
     1921 atgcggtctg ccgcctcata gcagtccgcc gtttcctccg gctcggcgca aaagacttcg
     1981 tccgcctttt ccagaatctg ttgaaaacgt tcccggagtt ccggtttcca cccggcgttg
     2041 tgttccggat aaggctgcac cagatgcagg cgcagcgccg gatggtagag tttcagcgca
     2101 cagatcatct ccgccgacca gaacgggatg ccgtatgcgc cgttgacata gaagtctgta
     2161 tatccctttt ccaccagggc gcagaggatc tccagcagat tgcacttgag aatggacagc
     2221 gcatttttcg gaaagcgctc cggcggagcg atctgcagtg ccagcagcaa ttcaaatcca
     2281 cgtgatgtaa tggtacaggt catcgcagca ctccccttct gtcgatttgc acccgttttt
     2341 tcagaacagg tctgcatctg tcaagccgtg aataacagtt tccatttaac tataccacaa
     2401 aattcccttt ttgtcaactc agctgagtta acctttcata cgccagattt cttataatga
     2461 acaatgaggg attcgctgga aagtgccgta agcgccggtt tcagcgattt tctttccaca
     2521 tcgttactag gagattcaat acatgacgtt aacggaagcg atccaacagc gtatccggga
     2581 actctgccgg gaacggaata taacgctgaa ccacttgtcc atgctctgcg gaatgaccca
     2641 gtctaccgtg cataatattt tcgcacgccc caacaacagc cccaccatat ctaccattaa
     2701 aaagatctgc gacgggctgg atatcaccct gggtgagttt tttcagacag agtatttcga
     2761 cacgctggaa caggaattgc agtagagcag caggaatgct gctctttttt tgcaatgatt
     2821 tttgcaggca atatcaaaaa ttgacgtttg atacaaagca tgatacaata aagacaacac
     2881 tacacaagtc tgacaggctg actttgtacg atgccgtctt aaacatgaaa tatcatataa
     2941 aggggatact atgaaacgaa cttttttatg caacatattg ttgacttctg cacttattgc
     3001 aatctgcatg gctagtttta ccggttgctt tcatgaaaac atacgtaaag atacctcgca
     3061 tat
//
LOCUS       DAWENO010000215         3039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10690_length_3039_cov_11.468834, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000215 DAWENO010000000
VERSION     DAWENO010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10690_length_3039_cov_11.468834"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          623 a          888 c          774 g          754 t
ORIGIN      
        1 ctgtctcgcc atggtctcga tctcggcaaa gctgtagttc tcaccctgcc gggcatattc
       61 ccggcgggac tcctggagta gccacagccc gatgatattt ttcagcagcg tcacctttct
      121 gccgtagccc acttcgttgg aaagcgccag cgcagccgcc tgctccgtca gaaccggctg
      181 ttccagctct gtgccgaaca gcgaccatgt gccgcagctc agaaagatat gccgctctgt
      241 cttagccggt gccgcaaatg ccgcacactg ggtgtcgtgt ccgcagactg ccgcaacacg
      301 aatcgccgga agtcccagcc gctgctgcac cgccggggaa agtgtgcctt tgtctgtacc
      361 gctttccaca atttccggca gaatccggtg gggaatgtgg aaaagcgaca ggagttcctc
      421 gtcccatgtg ccggtcttcg ccgagaccat ctgcgtggtg ctggcgatgg agcgttctgt
      481 cgaaaccgtt ccggtcagca gataggcaaa caaatccggc atcggcagca tcacttcgca
      541 gtcatccaac ctgccgctct cctggtcgca gatcagctgg aacagggtgt tgatctccat
      601 gacctgggtt cccgtccggg catacaggtc tgccgctgtg atggattctt ctgccttttt
      661 cagaatgccg ctggtgcggc tgtcccgata gtgccggggc agttccagga gcgttccgtc
      721 ggctttcagc agtccgtagt ctacgcccca ggtatcaatg gcaatgcttt catagggcgc
      781 aatttcatac gccgcctgca ttccctgctc gatctcacgc atcagctgag ggacgttcca
      841 gtacagtctg ccgtctttgg caacagggtc gttggaaaag cggtgtactt cccggagacg
      901 gatcttttcc ccgtcatagg ctgccagcat ggctctgccg gaagaggcgc ccagatccag
      961 cgccagtaca tagcggttca tgccatatcc tccggcagaa caacgccctt tttcagcagg
     1021 atgttggcat agatcgccgt ttcgccggtg gcaatgacgg catatgcctt cttggcacgt
     1081 tcatagaacg caaagcgttc catatattcg atctccggac gtttcggctc gtgcttgtcc
     1141 aggatgcacc ggtagtcctc ccagatcgtg ggtttcaccg ggtcacccgg cacaactgcc
     1201 atcagcccaa ccggtttttc ggtatagctg tccagaggca gaaagcgcat cacggcatcc
     1261 agaatctctg gcacgccgtg gccgtccaga cggatcagac gctgtgcaat ggcagcaccg
     1321 ggaaaattgc cgtctgcgat cacgatttcg tcgccgtgtc ccatttccat caacactttc
     1381 agcagttccg gggtcagaat cggcggaata tgtttcagca taagaccctc cgcttagttc
     1441 tgtccgttgg gatacttctt atatcccgga tgctttccgg tcacgccgta ctgcgcacgc
     1501 atactgcaaa gccggtcgat gttctctcgg gagatctcct gtgcgccgcc cagcagatgg
     1561 gcattgaggg agatcttcgc aaagagttcc agcgtttcca tgcggtagta tgctgtgata
     1621 aggtctgcgc ccactgtcag tgcgccgtga ttctggagca gcattacgtc atgctcctgc
     1681 aaatagggct tgatcgcctc cggcacttca tcggtagacg gcgtgccgta aggcgttacc
     1741 ggtacagaac cgatggcaat gaccgattcg atcatggtgt actcatccag cgccttgttt
     1801 gccacggcat agcccgtcgc caccggcgga tgtgcgtgca gcacagcgcc tacgtccggg
     1861 cgttcccggt agcagcaaag gtgcatcttg atctccgagg acgggcgaag tcccggagcg
     1921 cccttcagca cctcgccttt gtcgttgatc cgcaccagct tgtccggcgt gatgaagctc
     1981 ttgctcatgc cagtcggggt ggcgaagaac gtgccgtcct ccagcttgac ggaaacattt
     2041 ccgtcattcg ctgccaccca gcccagctgc cacatcttat gacagatgtc gcaaagctgt
     2101 tctttgattt cctgttcgtt catatgatcc tcctgtgtca gatctgctcc acctcgtgtg
     2161 aagtctgtct gtactgcgag cctgcggaaa cattctgcga atcccacagt ctctgctgca
     2221 aaacggcatc ggcagggcct ccaaatacgc cctgcacaaa ctgccctagt tcattataca
     2281 tttttcagat ggaattgtcc agctttttca gcagaaattt acatttattt tgatgaataa
     2341 tatgtttctt tctcatattt acaaacaata ttgtgaattg caaagggaaa aaatctttcc
     2401 gtttcggggc ttttttcgtt ttccggtgtt ttttcggaac agccgcagat acttttcata
     2461 aaaaacagca caccgaaatg tttcgatgtg ctgtacttct cttagaactt gttaatacat
     2521 ccaggtgtgc aaaccacgct gctttttgag aaataaaggg aaaatgtttg aaagtataca
     2581 agcatttggg ataatccccc tgtcagctta cgctgacatc ccccttttca agggggacat
     2641 tccaaaaagt acgccgacag acttttataa ctgtttccgc atggtcacat ggggacaaaa
     2701 ctcgtccatg tgaaaatcat ccgtctgggt atagcccagc ttttcataaa agcctgccac
     2761 acggcactgt gcggaaagtg caatgctcgc tgcgccgcaa gccttcgcct gctcttccag
     2821 agcagtcacc acacgttcgc ccagatgctt tccccgatac gacggcataa cggcgatccg
     2881 tccgatgatg tatgtttttc catcttcggt atacagccgt cccactgccg ccggcgtttc
     2941 gccgtccagg atcagtgcgt gataggctcg gtcgtcgatc tcgtcaaact cctgctgaaa
     3001 gccctgctct tccatgaaca cggctttccg gatgtacgc
//
LOCUS       DAWENO010000216         3026 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_10739_length_3026_cov_12.700101, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000216 DAWENO010000000
VERSION     DAWENO010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_3026_cov_12.700101"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          803 a          787 c          773 g          663 t
ORIGIN      
        1 gagtcttttg tcccccttga aaagggggat gtcagcgtaa gctgacaggg ggattaaccc
       61 ctccgtcaag cctacagctt gccagctcag cacaaggcac gcccttcggg tgccctgtta
      121 ggggagccaa aagaaaagcc tcctctccca aaggaggtgg caaaccgcag gcttgacgga
      181 ggagttccgg gcgaacattg ttcgccccta cgatgtgttt ccccaaaacg gcagtgtgca
      241 gggcaggata ccccatgtgg aaaaccgttt cacattttcg ttctgcaccg ctttccacag
      301 aaagttttcc cggttttcca catggatttt tggggaaatc aataagtttt caacaattag
      361 gggttgattt tccacaaagt atgtggtata ctattatatt agttacccct gcataatact
      421 gcgcctgcgt ctggttttcc acagcgcagg cgagaaaccg atagatacga ggaggattcc
      481 acgcacatga tttcatttga cgaagttttt tccagcgtga agcaatactg ccgggagaaa
      541 ggtgcgatcc cggatgtggc tgtcaagctg tggatcaacg ccctgcaccc ggtccgtctg
      601 gacgggaatg tggcgctgtt tacagtacag tcctcattcc agcgggacgt gatcctggga
      661 aactacggcg acctgctgaa agaggcgttt ctcaataccc tgggactcgc cgttgacatt
      721 cagatccagg tggtcgatga agaggaccag gaggtccagg agaagttcaa gaccaacgac
      781 tatgacgaga tcgcacgccg ccacgaggag ctggagcata cctttgaggg tgcaaactat
      841 gaatacacat tcgatacctt tatcgtaggc ggttccaacg aatttgccta cgctgcctgc
      901 accgctgtgg cgaaagaacc gggcaagaac tacaaccccc tgttcatcta tggtccgtcc
      961 ggactgggaa agacccacct aatgcacgcc atcgccaacc aggtacacga aaatcacccg
     1021 gactacaaga tcatttatgt gaccagcgaa gagttcggca acgacctgat ctccagcatc
     1081 aacgacaaca acacggcgct gttccacgac aaataccgga acgccgacgt gctgctcatt
     1141 gacgatatcc agttcttcag caacaaggag cgaatgcagg aagagttctt ccacaccttc
     1201 aacaagctcc acgccgaggg caagcagatc gtcatcacat cggacaagcc gcccaaggag
     1261 ctgaaaaccc tggaggatcg tatccgcagc cggttcgagt ggggactcat caccgacatc
     1321 agcacgccgg atttcgagac acgcatcgcc atcatccgcc ggaaggcaga gcttttggat
     1381 ctgtacattc cggatgatgt ggcagagttt atcgcaaacc gtcttaagac caacatccgt
     1441 cagctggagg gtgcagtgaa aaagctgaaa gccctcaagc acctggcagg ttcgccgcct
     1501 tccatttcca tggcgcagag cgtcatcaag gacattctga gcgacgacca gccggcgccg
     1561 atcacggtag agaagatcat cgtagaggtt gcagacgttt acggggtttc tccggaggac
     1621 atccgcagca gcaagcgttc cagccagatc tccacagcga gaaaggttgc gatctatgta
     1681 gtgcgtgaga tcacgcagat gccgctccag gcgattggca cagagttcgg cggcagagat
     1741 cattctacca ttgtatattc catcagcaac atcgagactg ccatgaagca cgacgatcat
     1801 ctgaaacagc tggtagagga catcatcaaa aacatccgca gcaaaagcca cagctaagcg
     1861 gcgggtcagc ccgtcgccca gcggaaatgg cgacggcaga cttgcccaag gatagcaagc
     1921 cgccgctgac ccttcccccc aatttagcgg tcaagaaagg cagtgttacg gcggcagaag
     1981 ccgcctgttt gcccctccgg gggatttatg tttctggtaa gctgtgaaaa cactgccagt
     2041 cgtggcatac actgccgttc cgaacaaggg taggcgagat cttttctttt tttgcttctt
     2101 tttttctttt cagcaaaggc agcatgcttg ctgctgagcc tttcccccca atttaacgtt
     2161 caagaagggc agtgttacgg cagacaagct gcctatgacc cctccggggg atttatgtct
     2221 gaaaaaccta ttaaatatgc cacctcttat ggtaaaaggg tgtgaaatga ggttggactt
     2281 gtccacaggc ttgacggagg agtcacacac actgccagtc gtggcataca ctgccgttcc
     2341 gaacaagggt aggcgagatc ttttcttttt ttgcttcttt ttttcttttc agcaaaggca
     2401 gcatgcttgc tgctgagcct ttccccccaa tttaacgttc aagaagggca gtgttacggc
     2461 agacaagctg cctatgaccc ctccggggga tttatgtctg aaaaacctat taaatatgcc
     2521 acctcttatg gtaaaagggt gtgaaatgag gttggacttg tccacaggct tgacggagga
     2581 gtcacacaca ctgccagtcg tggcatacac tgccgttccg aacaagggta ggcgagatct
     2641 tttctttttt tgcttctttt tttcttttta gtaaagaaaa aaagaagaac atctccttgt
     2701 gtcaatttcg cacaggaaag caaaagagaa acaagcgcag caaacgctgt atcaaagcaa
     2761 aaaccatccc caaaacaaac acccggaata tcaacacagc acatacaaga aaaggcaaac
     2821 aaaagcgttt gcaagcccaa ggcggacagc gagagaaacg agcgattccg ccggacgggt
     2881 ttcggaagtg agacctggga tatcgtttac cggaataaga aaagattgac gggcgaacaa
     2941 tgttcgcacc tacggtaatt gttttagaaa agtccccctt gaaaaggggg atgtcagcgt
     3001 aagctgacag ggggattcac ccctca
//
LOCUS       DAWENO010000217         2917 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11185_length_2917_cov_8.604822, whole genome shotgun sequence.
ACCESSION   DAWENO010000217 DAWENO010000000
VERSION     DAWENO010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11185_length_2917_cov_8.604822"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          766 a          719 c          646 g          786 t
ORIGIN      
        1 cgtttactac ccggatttca tcatttccac gttcaaacac ttgtatcata tcaccgtcca
       61 gcggatcaac aacttctttt ttcactcgga aatccggaaa agtttttgca atgcgtgcaa
      121 cagtttcatg atatttttcc gtgttatatt cgccgcatat cgtataatca aaagccattt
      181 atgccacctc ctaaagtcca gcacgtttat tctacttctt tcttttctta aaaggaaaga
      241 ttttcatatt cttcaccgta ttttgtgcaa tgctttcgca tggagtctag tgcttcaaat
      301 cttatttcta ttataccatc atccactcta gaagtcaacg aattcacgct aattctctcc
      361 ggcaccacgg gtgcatgggc ggaaagttca gacccggaac gctctccgag atgttgaacc
      421 gctcgccgcc acgcaccttt tttgaagttc atcaaatcca aaatctcacg cagtttccat
      481 atgatacaga cttttttcaa aaaccgcttg acatttttga aaatatagtg tactatatag
      541 ctggtgaact agtcacccaa taaatcagaa aggaggtcac catggacttt caagcgaatt
      601 atcccattta tctgcaaatt gcagaggacg tgaaacggcg tattgtgatt ggtgaactaa
      661 agcccggtga caagctgccc tccaatgcgg aacttgcaat tgcctaccag gtcaacccga
      721 atacggtaca acgcatctat cgtcagctgg aggcagaagg tatttcttat accaaacgag
      781 gcatcggcac gtttttggcg gaagatccgg caatgccgga acgcttgcgg caggaaatcg
      841 gcgaaaaact cagcgcccag ttcttaaagc aaatggaaac ataccatttt tcaaaagaag
      901 aaattattcg cctgttagaa acacaggcac agaaagaagg agaattatgc tgaaatttga
      961 acacgttaca aaagtttacg gcggcaaaaa agcattaaac gacttgacac tttccttttt
     1021 gcccggtcat gtttacgctc tggtcggacc gaacggcagc ggaaaaagca caatgatgaa
     1081 ggttgccgca ggactggtaa aacccaacag cggcagcgcc acctatcagg aaatgcccat
     1141 cggtgtggaa agcaaaaagc acattgccta tatgtgcact gagccattct attatgatta
     1201 tatgaagatc caggatgtca agcagtttta tgcagatttc tttgcggatt ttgacaaaga
     1261 acgcttcgac tccctgctcc agttcatgca gctgacaccg gacatgaagg ttcgctccct
     1321 gtcctctggt atggctgcca aactgaaaat tgccgcaaca ctggcaagaa atgctgacat
     1381 ttgtatgctg gacgaaccgc tgaacggcat cgacctggta ggacgggatc agatcatcca
     1441 gagtattctt cgtgcggcaa atccgaatgc aaccattctg atttccagcc acctgtttga
     1501 tgaactggaa ccgatcgtag accacattgt tatgatggcg aacggctctc tgattttaga
     1561 gggcgaactg gaagagatcc gcacacggta cggaaagtcc atttcggatc tgtaccggga
     1621 atatttttcc ggtactgctg catccccgct gtttccgaac aacacaaatc aacccactgt
     1681 ctgaatatcg aaaaggagat catactcatg ggaactttat taaaatacga attttgccgc
     1741 tcccgcacaa ttttcctggg aatcatcggc gttacgcttc tgatcgaatt ggtttatctg
     1801 atcggattct ttacaaagat aaaggcgctc tttgccgttg gcctgatcgg cggcattctc
     1861 tgtctggcac tgggcgcagc tgcaattctg ctgtacggcg ttatcatgtt taatgatgac
     1921 atttccaaaa agcccggcta cctgctcttt tccacgccgc gcagcgccgc acagatcgtg
     1981 ggcgcaaagc ttttgatgac gctgtttgcg ctggtcggga tcacaatctt gttttcgctt
     2041 ctgatcgctc tggacgtatt cctggcgctt cagcgaaacg gaacctctct cgttgccctg
     2101 ctgtctatgt ttgacgcatc catcacagag aacggcttga aggatgttct gttcaacggc
     2161 tacaatttct tcggcctttc tatgtatctg ctgaacactg tgatctcgtt tatcttcaat
     2221 gtgatcgccg catatgttgt gatcgttctg ctgaaaactg tcatgggcaa tcagaaggga
     2281 cgcacgatcc tggcagtgat tctctggttt gcaattacaa acgcattcaa cctgctgggc
     2341 ggactgatta ccacacagct ggcatccgga ctggacgaag gaaatgaagt tgttgttaca
     2401 caatccagta cactgtttct cagcacctcg cttcacacac ttttccagcc tgccatgtat
     2461 ctgcccacaa tgatcctctg catcatcggt tccattgccg gctactggct gaccacctgg
     2521 atcattgaaa agaaactgag tgtatgacgc catgcaaaag gaaatcaaat cagacaatca
     2581 tgaccctgca tcggacagcc cggagcaatc gtatattctc cccggcatct gcatcggaat
     2641 gatactggga atgcttttgg gttttgccct gggcagtctg gtgttccaca acattatcct
     2701 tgggttatgc accggacagt ctgccggtct ctgcctgggt attgcggtcg gcgccgctat
     2761 tccaaaacga aaataatttc aaggcaacac cgcacagagt ttgtgcgtaa gatgcgcagt
     2821 cggatttttc ggcagattgc acagaaattt cgcaggcata ctgtcgtatg tcaagaaatt
     2881 tctgtgcggt atgacggaaa atcagcaagc agattgc
//
LOCUS       DAWENO010000218         2912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11205_length_2912_cov_8.602730, whole genome shotgun sequence.
ACCESSION   DAWENO010000218 DAWENO010000000
VERSION     DAWENO010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_2912_cov_8.602730"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          891 a          567 c          698 g          756 t
ORIGIN      
        1 cttttattat actacttttc ttttcccttg tctacttttt tagtataaca ccaattccga
       61 acagcgtgac cagcattgaa tatgaggtat tctctgactg tacaagcctg accagcatca
      121 ccattccgga tggagtgacc agcattggaa atagtgcatt tcgctgttgt agaaatctga
      181 ctgctctcac acttccgaac agtgtgacca atatcgaaga atacgcattt ctttattgcg
      241 aaagcttgac cgacatcaca atttcaaatt gtgtaacccg gattggcagg gaagcatttt
      301 ctaattgtaa aagtattaaa attgctgttt ctgataagag gcatgaaagc actaatcgac
      361 agcggcaatc tggagctgat tcaggtgctt ctggaaaaga ccgactttgt taccaagaag
      421 aacattgata aatttatcca gtacgccatt gacaccaagc agcaggagat ctatctgacc
      481 ttgattcact ataaggacga gatcggggcg tatacagata tttccaagaa gttccggctg
      541 taatagagca gaaagggaag tataaccatg aatattcatg aagcgaaaga gcaaatcaaa
      601 aacgcagtga ctgcttatct gacaaaggat gatctgggac gctatgtgat tccgccgcag
      661 aaacagcgtc cgatctttct catggggcct cccggtgtgg gaaaaactgc cattatggaa
      721 caggtcgcct cggaattggg catcggtttg ctgtcctatt ccatgacgca tcacaccaga
      781 cagtccgcat tgggactgcc gctcatcaag catgtgaact atcagggagc gagtacgatg
      841 tttccgaata taccatgagt gagatcatct cttccgtcta tgaactgatg gaaaacaccg
      901 gcattcaaaa cggtattctg tttctcgacg agatcaactg cgtttcggag acactgactc
      961 cgatcatgct gcaattcctg cagtataaag tgttcggcag acacgaagtc ccggctggct
     1021 ggatcgttgt cactgccgga aatccaccgg aatataaccg ctctgtccgg gagtttgaca
     1081 ttgccacatg ggacagactg aaacgagtag atgtggaacc ggattttgcg gtgtggaagg
     1141 agttcgcata tctgaatggg gtgcattcct cgattctgac ctatctggaa atcaaaaagg
     1201 gaaatttcta taaggtggaa actacggtgg acggcaagcg ttttgtgacc gccagaggtt
     1261 gggatgattt gagtcagatg atccacctgt atgaacagca caagattccc gtcgatcaga
     1321 aactggtgga acaatacatt cagaatccgg aaattgcaca ggatttcgcc atttactatg
     1381 acttgtacaa caagtacaaa agcgactatc agatcaaaga cattctggca ggtacagcaa
     1441 gcaccgatat tcaggagcgt gccaaaagag caaagtttga tgagcgtttt tctctggtgg
     1501 gactgctgct ggatgctgtg atgggtgcca tgcgggagtc tacggaagaa gaggacgtat
     1561 tggaagaatt ggtcaagacg ctgcgtgtgt tcaagcggga atccaagacc gaggacggcg
     1621 cagcgctgct gaaaaaatac atccggaaca accgaaaaga actgaacgcc ggcattcggg
     1681 ccaaaagtct ttctgaggca cagcagaaac aaaagctgca agtgatgcgg attctggagg
     1741 agcagagcaa actggtgcag tctgatccgg agcaggcagc ttttgcactg gtgaaaaagg
     1801 atttcgataa tcggttgaag gcattccaaa agcgaagcga cacactgtcg gaacaggtgc
     1861 ggcatatgtt tgcgttttgt gatacggtct ttcccgatgg acaggagcag ctgatcctgg
     1921 tgacagaact gacggcgaac ccgtatagcg caagattcct gggagaacac aaggtcgatg
     1981 catattttgt ccacgataag gaattgatgt tccatcagag acagctggaa ctgaaccagg
     2041 aaattgcgaa tctggagctt taagagaatt ttctgcatat gttgtttaaa attgtagtat
     2101 acaaatccaa tcaaaactac aaaagcacac agggcatttc aaaatagctt tgtgtgcttt
     2161 ttcttgtaaa tttttttaga acagacagac gtatgtctta cagtatgagt ataatgaagt
     2221 caaagataaa atgagaagtc tgacgaactt aaataaaatc ataagatttc tgctttcggc
     2281 gtttttttat aaatattata ataaaaaatt gacaaaaaag agattgtgtg gtatacttat
     2341 agatacatag tatttaccaa atggttgaat tcataagaga ggatgggata tcttttggaa
     2401 ggactgataa acaagcaaac agacacctct gttataagat atcgtgataa atgcgataaa
     2461 tatgattact taattgcgat tggaacagga gcaattgcag gaatcgtaga tattatgttt
     2521 gtaggttctc cgacggacag caagctagga aaatggactg atgcacaggt agataactgt
     2581 gtaaaagcat ttgctaaaac aagtggctgg agtccaagag ccggaaaaga aaatagtaca
     2641 tccagtgcaa ttggatttct tgaaaaaaag ttttctgtaa attatgatca gcggcacagc
     2701 ggcgatgttg gcagagcttt ttccatgagt gccaaaaatc atcatatgaa atctctggca
     2761 cactctccgg atgttatagg cttgttcttc tcgattctta gccaattcac gggaatatct
     2821 gcttttctgg caaatggcaa attgattacg atatcctcgg aaaccatgga actgcaaggc
     2881 ggaaatttta tcgcaaaaat tttctctggt gt
//
LOCUS       DAWENO010000219         2909 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11217_length_2909_cov_5.796076, whole genome shotgun sequence.
ACCESSION   DAWENO010000219 DAWENO010000000
VERSION     DAWENO010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11217_length_2909_cov_5.796076"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          673 a          768 c          763 g          705 t
ORIGIN      
        1 gtgtatttga acccgaccag tgtccccaga ttcagaacca gcccaagcgc caggagcagc
       61 tttgccggcg ttttgtcccg gaatcgttca atgccacgcc ccaggacgaa attcaccaga
      121 gcgctcgcca cgaccagcca caggaaggaa aggtcaccgc aggcgtagaa cagcagagaa
      181 aagacgatta gcactgcatt gcggtaggac gttttcttcg tggcaaagta gcagagaaaa
      241 cagatgggaa agaatagata gatgaaaaac aatcgggaaa aaaccatatg ataagacctc
      301 ggttcgtctg tattttttta tttttatgta gtacggagcg gttttccagc aaggaagcaa
      361 taatcgcttt ctttgccgct acctcttatt atacaacaaa atgcggcagg attgcaaggg
      421 gaacgacacg aagaaacggc tttcccggaa aatcagcttt ttccacaaaa aaagacggca
      481 aaaagccgcc tttttcggta ttgtctgatt tttggaaaat cggaaaaatt tactgtttgg
      541 tcggtgcagc tgttgtggtt gctgcggtgg tcgtttcgcc agccggagca acatcggaaa
      601 gaacgccctt gagaccggca gccaaaccag ccagaccctg aatctcgctg ggaatgatga
      661 tcttggttgc cttgccgtcc gcagcctttt cgaacgcctc cagagatttc agcttcagaa
      721 ccgactcgct ggggtttgcc tcgttcagct tgaccaggct gtctgccagt gccttctgta
      781 ccagttcgat tgcctgtgcc tcaccgacag cgatgcgcat tttctgttcc ttgatcgcct
      841 cgcctcgcag gatcacagcc tccttgtcag cctctgcctt caggatcgca gtctgcttgt
      901 cagcctctgc acgcaggatc ttggcgtcct tttcaccctg tgccacagtg acctggtatt
      961 ccttttcacc ctgggcacgc aggatctttt cacgcttttc acgctctgcc ttcatctgct
     1021 tttccatggc atcacgaatc tcagccggcg gcaggatgtt tttcagttcg acacggatga
     1081 ccttgatacc ccagcggtct gttgccagat ccagggtctc tgtgatacgg gtgttgatga
     1141 ggtcacggga agtcagcgtt gcgtccagtt ccagttcacc gatgatgtta cgcagcgtgg
     1201 tagccgtcag attctcgatg gctgcgatag gatgctctac gccgtatgca aactgtacag
     1261 agtttgtgat atggaagaac acgactgtgt cgatctgcat ggtaacgtta gccttggtga
     1321 taactgcctg gggtttgaag tcaactacct gttccttaat ggagacccgt tttgccacac
     1381 ggtcgataaa cggtaccagg aagtggatgc cggtgcccca cgctttatag aatgcaccca
     1441 gacgctcgac aacgtagacg tttgcctggg gaacgatgcg gatacgggaa accagtacca
     1501 gcacgataaa tacaagtact gctaaaatca caagaaatgc cataatgttt tctccttttt
     1561 gaatccatgt tgcaatgttt ttggtacgct gagcgtgtgc ttactgtgct gtgcctgcgg
     1621 tgacagtctc cggcagacgg ctcacggtga gctttgttcc gctgactgcc tcaatgcgga
     1681 cgctggtgtt ttccgggatg atcgtgccgt cctgggaaac tgcgatccag tccacaccat
     1741 ccaggcggac acgtccggta ttcttcaccg gatcaacttc ctggattacc acggcgatgc
     1801 gtccgatttc ctggttattg gtgtccttga tgccgaaaga aaagatcctg cggcagatgg
     1861 gacgggtgaa gatcagcagc agcacggaga ccgccagaaa caggataccc tgcaccggca
     1921 gagatgcgcc aaagcagacg gcgagaaacg ctgccagcga tccggctgca aaccagatgc
     1981 tcaccagctg ctgtgtggcg aattccacca gaacggtcag tacaaaaaga ccaccccaca
     2041 gcagaaacgg catataggct tccatgcaaa actcctttct gtcatttgag tcggtttcgt
     2101 aaaaccaacc atgctctaaa aaagccttga tatccttttg cgtctggata tcaaggctag
     2161 tatatcatat ttttgcagtt ttgtaaagcg tttttttcga attcccggtg aaatccaggt
     2221 aaaatgcccg ttcagagaga ttccgggtgg atacccagcg tttcgcacag cacccgaacc
     2281 acgccgtttt tggtattggc aggtgcggta tgtctcgcct ggagcttgac ccggtcggag
     2341 gcattctcca tggcgtagct gaactcagcg cattgcagca tggtgatatc gttgtcatag
     2401 tccccgaaag ccatggtctc cgccggcgta atgcccatgc gttcctgaag ttcccggagc
     2461 gcagcgccct tggtgatgcc cttgcacata atgtccatcc agcagtcgcc ggaaatgagc
     2521 agctcgtact tatcccccag ggcatcccgc atggcaggat atgcctcacg ggtcggtccg
     2581 ttggtgtcgc acagggcgat tttcaaaatc ggctcgtcta ccgcatagag catatcatag
     2641 ggaacaacct ggggatcacg gtaataccgc tggatctcac gctgcatggc ggcgttgttg
     2701 ttttccgggt aataaatgtg gtggaaaccg cacagcaccg gaacggcggt ggacagctga
     2761 tgacaggcat ccagcgtctg gcggatgact tcctttggca ggctgtgcag catgggcgcc
     2821 tcatgcggat acatgacgca cgccccgttg tcgcagatga aaatgatctc ttcggcgagt
     2881 tccccaaaca gattggtcag catcatatg
//
LOCUS       DAWENO010000220         2901 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11251_length_2901_cov_8.906184, whole genome shotgun sequence.
ACCESSION   DAWENO010000220 DAWENO010000000
VERSION     DAWENO010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11251_length_2901_cov_8.906184"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2660..2669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          817 a          657 c          740 g          677 t
ORIGIN      
        1 gatcacacgc tgcagatgcg tgtatgggaa cggggcagcg gtgagacatt tgcctgcgga
       61 accggcacct gcgctactgt tgttgcagca gttctcaacg gttattgcaa gaaggaagaa
      121 gaaatcctgg tacatctgcg tggcggcgat ctgcgcatca tctaccatga agacgaaaca
      181 gtcaccatga tcggtcctgc tacctatgta tttgagggga aaatggagta ccccaagctt
      241 taattttgta tctattattt atttttacca tatgaaaagg agtatgtcaa atgccaacga
      301 tcaatgaaaa ttttctgaag ctggaaaaga actatctgtt catcaacatt gccaagaaag
      361 tcaatgcttt catggcagag aatccggatg caccgctgat ccgtatgggt atcggtgacg
      421 ttacgctgcc aattgcaccg gtctgcgttg aggcaatgaa aaaaggcgct gacgaaatgg
      481 gagttaaaga gacattccgt ggttatgagg acagcggttc cggttatgat ttcctgaaaa
      541 aggcaattgc aggatactat gaaaagttcg gcgtttctct ggaactggat gagatccgtg
      601 tcaatgacgg cgctaaaagc gactgcggca acattgtaga tatcttcggt gatgataaca
      661 tcgttctgat tacagatccg gcatatccgg tttatgtaga ttcaaacaag atgaacggca
      721 gaactgtgat ttatgcagat tctgacgagt ccaacggttt tgcggcaatg ccgaatccgg
      781 aggttcacgc tgatctgatc tacctctgct cgccgaacaa cccgaccggt tctgcatata
      841 ccagagacca gctgaaagaa tggatcgctt acgcaaaggc aaacaaagca atcattatct
      901 tcgatgctgc atatgaggca ttcattactg atccggatgt tccgcacagc atttatgaag
      961 taaaaggcgc aaaggaatgc gctattgaaa tgtgctcgct gtccaaaact gccggtttta
     1021 ctggtatgcg ctgcggttac acaatcattc cgactgctct gcacgtaatt gcatctgacg
     1081 gaacagatgt cagcatcgca cagatctggg gcagaagaca gggaagcaag ttcaacggcg
     1141 tttcttatcc ggtacagtgc gctgctgctg ctgttttcac agaagaagga cagaaacaga
     1201 ttcgaaagaa cattgcgtat taccaggaaa atgctgcaat cattgcaaag acaatggaag
     1261 aactgggaat cgaatacacc ggcggcaaaa actccccgta tatctggctg aaatgcccga
     1321 acggcatgaa gagctgggaa ttctttgact atctgctcca cgaggctgct gttgtcggca
     1381 caccgggcga gggctttggc aagaacggag aaggctggtt ccgactgact gcattcggag
     1441 acagagacaa gacaaaggaa gcaatggacc gcatgaaaaa gctgcttgca aagtaagcaa
     1501 atacacaaac aaatcaattg gaggattatt ttattatgcg tgctgtaatc acagtaatcg
     1561 gtaaggacaa tgtaggcatt ctgcacaagg taagcggcgt atgcgcaaaa tatgaggcaa
     1621 atgttctgga agtaacacag agcgtacttc aggatatgtt tgctatgatt atgatggtag
     1681 atatcaccgg aatgacaact gacttcaccc agctgagcga cgaactcaat ggactgggcg
     1741 agacactggg cttgtcaatc cacacaatgc acgaagatat tttcaacgca atgcaccgca
     1801 tctaacaact gaaacgcagg agaagaatta tgaatagcac tatgtttaac gcaggagata
     1861 tcctggaaac aattcggatg atccaggatg aatgtctgga cattcgtacc acaactatgg
     1921 gtatttctct gctggactgt attgaccctg acatcaacaa ggcgtgtgac aaggtctacg
     1981 acaaaatcac ccgaaaggca gaaaagctgg tagaaaccgg tgaaaaaatt gaaaaagaat
     2041 acggcatccc gatcatcaac aagcgaattt ctgttacccc gattgcaatg ctggctgctg
     2101 cctgcccgaa agacaatccg gtaaaattcg caaaggcact gcaaagagca gctgaaacct
     2161 gcggcgtcaa ctttgtcggc ggttactctg cactggtaca caagggattc agcgccggtg
     2221 atctggaact gatccgctcc atcccggagg ctctggatgt caccaccaga ctttgctctt
     2281 ctgtcaatgt tggttctacc agaagcggta tcaacatgga cgctgtccgc atcatgggcg
     2341 acattgtact ggaatccgca aagctgacag cagaccgtca gtgtgtcggt ccgtctaagc
     2401 tggttatttt ctgcaatgca ccggaagaca atccgtttat ggctggtgca ttccacggtg
     2461 tcggcgaacc agactgcgtt atcaacgtag gcgtttccgg ccctggtgtt gtacgttcta
     2521 ccgtatccaa gtatccggat gcgtctatcg atcagattgc agacatcatc aagaaaaccg
     2581 cattcaagat cactcgaatg ggtcagctgg ttggcgcaaa agcatctcag atgctgggcg
     2641 ttccttttgg tatcgtagan nnnnnnnnna caccggctgt cggcgacagt gttgcacata
     2701 tcctggaggc aatgggtctg gaaacctgcg gcacacacgg cacaacagct gctctggctc
     2761 tgctgaatga tgctgtcaag aaaggcggcg tcatggcatc ctccaatgtc ggcggactct
     2821 ccggagcatt tattccggtt tcagaagatg ctggcatgat cgacgcagca gtcgacggta
     2881 cactgactct gacgaaactg g
//
LOCUS       DAWENO010000221         2865 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11420_length_2865_cov_7.884698, whole genome shotgun sequence.
ACCESSION   DAWENO010000221 DAWENO010000000
VERSION     DAWENO010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11420_length_2865_cov_7.884698"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          667 a          723 c          757 g          718 t
ORIGIN      
        1 cttgtccgtg cggcaccaaa aaagcgccgt acagggtgtc acaacatcgc agccgcccac
       61 agatcccggc tatctgatgc ggatcgcaga cggcaaggtc gggctgtacc gcatcgggag
      121 cagtaccccg tatgagctgc tggatgagcc gctgaacctg ctttcggaat atgaccggga
      181 gcagctggag cagggaatca ccgcatccag tgagggcgaa ctgcgccggc ttttggagga
      241 cttcttttca taagcacaga aaaagcgcct gtttcgtatc cctgtggggg gaaaacaggc
      301 gctttcggca tattcaaaga gaaatgggaa ccggctttcg cagtgtcatc gaatccggcg
      361 tgacatcgca gtccattgcg agcagcttga caccggatgc cgcagcgagc cggagcgttt
      421 gtgcaaattc cgggtctgtg gcatcattgg gacgaaactg cacaacatca cgcatttgta
      481 ccacaaacag tagatatgca ccgaatcctg ctttccggca ggcaatcagt tcccggagat
      541 gtttgacgcc acgctgtgtc ggcgcatccg gaaaggagac aacgccgtcc tgttccagtg
      601 tgacgccctt cacttccaga aaggactgca tttcgccgtc tgtgattctg aagtcgaacc
      661 tggaattccc ataggtgact tctcgctcaa ttatggcatt ttcggagaaa agcgtaccct
      721 tgcgcagcca ttcctccgca gcatcgtttg gaatctggga gtccagattg atgagcaggg
      781 gagtctttcc ggggcgctcc ttttccactg cgatcaaatc gtatcgggtc tttcggttcg
      841 gattggtgcc atgtgccaga tagaccttgt agccggggac aagcagttcc cggcagcgcc
      901 cggtgttctt gacgtggaca cgttctgtcc gcccgtccag atcgaccatt gcaataaaac
      961 ggttcggacg ttctagaaac gtgccggcgg ttacgctttg gtactgcatg atgcctcctc
     1021 attttccggc aggaaaacct gtactgaaat tgtgtattcg ttgccggtac agctgatctc
     1081 tgccgtcccg tgatattccc tggcgatttt ctccagaaca ttgtgccggt ctgccaggtc
     1141 ggattcattt tccgcaaagc actgtacctg gatgcagaca gatcccttgt cctgctgcac
     1201 gttgcaggag atacagcgct gttcctccgg aagtttctgc actgcctgca tggcgttctg
     1261 gagcggatgc aggaacagac tgcaaaggtc gatcttctga atgggcaaat cgtctgccag
     1321 actgcactgc acctcaaacc ggatgttctg ggtagcacac tgttcggcag tctgctgtaa
     1381 gatcacattg gcaatcttgt tttcacagta atgcagccgg agcgcctggt cctggaattt
     1441 gctgtgcagt tctgcggcaa actgcttggc atcttccagc tcgtttgcgg cgatcagccc
     1501 ggaaaacacc tgcatctggt tgttgaagtc atgccgcatt tttgccgcct cgtgagagac
     1561 tcgtcctgcc tgttcctggt attgcaggga aagcgcttca tactgttcgt aaaactgcaa
     1621 tttttgtgcg atctgttcct tttcctgcat attccagatg aagtggaaca gcatgacatt
     1681 ggaaaccacg cccagacaaa atgtaattgc cacgagcagc aggataatgg gagaagtgga
     1741 catgatccga tacttctgga tgataaaggt cagcgtcaga acaatggctg cctgtgcagc
     1801 gggaaagacc aggtagggga gtttctgcgt gatttgttcc atcagcgtcc gcatttttct
     1861 ccgatgcaca aattttttca ggaagtggag aaagatcaga tagaacgtga ttgtaccgat
     1921 atagccgata aagcaatacc acttttccca tcgctcatcc gccatcagca gaactgccgt
     1981 gtgatatccg gtgatggcat caaatacgct gaagagcagg acgctcagca catcacaaaa
     2041 cgacatggaa acgcagttga ttaggaaatg gaacaaccgt ctggaaaccg gatggtttcg
     2101 ataggcggcg agatagtaga caagatagga accccagcta aatatattga acagtattcg
     2161 caggtcattc agcatcagca cagcgacatt gccgtacatg atgtaagaaa cggcaaaaaa
     2221 ggcaatatgg atcaacaggc taaaagaaaa cagtgctgtt cttgctgtgc ggcttgcctt
     2281 tcccagtgtg atattggaat agaggagtac aaatacaacg acacacattg cggaaaaagt
     2341 cagattaccc atttcccaga atatttttaa tgtgtgcatt ccactgcctc cttttccgat
     2401 tcctccagcg gtgcagtttc cggcagattc agctgaatga caatggtgaa gaactggtcg
     2461 tcatagctgg catctgctgt gccgccgtag gactttgcaa tgtgttccat gatgtcgatg
     2521 ccgaatccgt gtctggactt gtcctgcttt gtggtctgga atttgccgtc ctgcacctgg
     2581 acttcgtttg ccttccggtt gcggatgcgg aaaaagacca tgctgttttc ctgccagatg
     2641 ctgcacgaga tctgccgttc tccctccggc actttttccg atgcctggat cgcattttgc
     2701 agcggattga ttaaaaggct gcaaagatcc acccggcgga tgttcagcat ttccggcagg
     2761 gaacagcgga cttcaaagtt gatctgcttt ttgcggcagg tctctgcggt ctgctgtaaa
     2821 attacattgg caatggtgtt ttcgcagtac tgcagtttca gcttt
//
LOCUS       DAWENO010000222         2864 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11422_length_2864_cov_10.802421, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000222 DAWENO010000000
VERSION     DAWENO010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_2864_cov_10.802421"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          645 a          806 c          694 g          719 t
ORIGIN      
        1 cagtttttca aacgatttaa ttcatcataa tccactgcgt ttgaaacttc tgccatccaa
       61 aaaataagtt ctccacactg aattctctta ttaaattgag catcatcttt ccgccgtttc
      121 gaccatgact ctattaaatg ccagtattgc gtaggattat ctaccttgca ttccttagca
      181 tatttaatat atccacctga tttttgtctg tatccatgtg aaattaaatc gtccgtcaaa
      241 gggcatggaa cattttcctt tcctgcataa aaattgcgta ttttccaatt ttccataact
      301 gaatctctcc aaattttcgg ttcacatctt tcactcgttt tcccagcact ttctcacccc
      361 aacaacgacc gatacaactc cacatacttc tctgccgaaa cgttccagct gtagtcgcac
      421 ttcatggcgt ggcgtaccag agcgttccac ttccgcttgt cggcgaacag ctcgcaggca
      481 cgcttcacag catccagcat atcgtatgcg tcataggtct tgaacgtaaa gccattgccg
      541 ttcttgccgc cggcatcgaa aacagagtcc ttcagaccgc cggtttcccg taccaccgga
      601 atggtgccat accgcatggc aaccatctgc gccagaccgc agggttcgtt ctgggacggc
      661 atgaggaaaa tatctgcgcc gccgtagatg cgccgtgcca ttgccggcac aaatcccacg
      721 gtcacatgca cctgatccgg atactctgcc tgcatttccc ggaagaagtc ctcgtacagc
      781 cgctcgccgc tgcccagtac cacgaaccgg cagcccagct gcagcatttc ctggaaggtc
      841 tgctgtacca gctggatgcc cttgtgtgcc acgaaacggg tcacaatgcc cactaccggc
      901 gcatcggaat cggtcagacc gcattcatgc agcagggcag cccggcactc ttttttgccg
      961 gcgatcttgg tcacggaata atgctttttc aagttggagt ccgtctccgg gtcatagccc
     1021 acgacgtcaa tgccgttgag gatacccaca atgccgtcct gccgccgcac caaagcacga
     1081 tccatgccgt aggaatacca cgggtcgagg atctctttcg cataggtggg gctgacagtg
     1141 gtgacacggt ctgctgtttc aatagcgcct ttcatcagat tcaccatgcc gtcaaattcc
     1201 agcaggctgg tgtgataggt gggaatgccc atcagctctg tgacaacttc cttgccgtaa
     1261 acgccctggt actggatgtt gtggatggtg tagataatgc ggatgttctc gaattcctgg
     1321 tggtattgat agagaacacg gtagaacacc ggcaccatag ccgtctgcca gtcgttgcag
     1381 tggatgatgt ccggtttcca gtccagcgcc ggcagaattt ccagcacggc acggctgaaa
     1441 aagacaaagc gttcgcagtc gtcgtaaaag ccgtacattc cgtcccgcat gaaataatac
     1501 gggttgtcca gcaggtaata ggtcactttg cccacctgga tggtgtagat gccgcagtac
     1561 tgcttccgcc agcccacttc tacggtgata tccttgagat aggtcatctt gttgcggaca
     1621 gccggggtga cgctccggta caggggcata acaacacggc agtcgatctc gctgttcttg
     1681 ctcagcgcag ccggcaggga gccagccaca tctgccagac cgccggatgc ggcaaagggc
     1741 agtgcctcgc tggcagcaaa taatattttc atttccaaca ctccttttat gacggcaacg
     1801 ccgcacacac aattttgcgg cattgcaaaa actgccgtat gaaccgtttc acaggcgctt
     1861 gcccgtgtat gccggtcgca tacggcagaa atttctattt caaatgagat cccaaacgca
     1921 gaaaacagcg cttagatggt tgccttcttg cccagataca gcggatattc cggagaaccg
     1981 gtcagaacac ggtttgcaga aatggtaaca ttcttgtcgg caacgatggc gtcaatgttg
     2041 cagccctcgc cgatctcagt accctgcatc agtacgcagt tgcggacaac agcacccttg
     2101 gcaatcttca cgccacggaa cagaacacag ttttctacgg tgccttcgat catgcagccg
     2161 tcggctacca gggaattctt cacgctggat tccagaccgt attttaccgg tgcattgtcg
     2221 ccgaccttgg tatagatcgg agcctcgctg ttgaatacgt tgttgcgtgt cttggtgtcc
     2281 agcagcgcca ggtttgcctt gtaataagcc tccagactgt cgatcttgaa gaaacagccc
     2341 ttatgctcgt atgccttaaa gacaaactcg tccttgcgtg cctggagcag gtcacggatg
     2401 aggctcttct ggttcttgct gattgcctcc agaacgacct gtttcaggaa gtcggtggtc
     2461 atgatgaaca tatccagaga gatgttgcac tcgccggcaa gcatgggatt ggtcagaact
     2521 tccttgatgg tgttctcctc gtcgatctgc agaatgcagc cgtttgtgct tttatcccgg
     2581 ttgtacagtc ccttggcata aatcacggtg atatcagcgc cgctgttctg atgctgtgtc
     2641 agagcgtcgg taaagtcgat ggtcgtaacc agatcgctgt ttgccagaac tacatattct
     2701 gccttggtgt gttctacaaa gctccagatg ttgctcagtg cctccagacg tccctggtat
     2761 gtaccgccgc cgacctggct gaacggcggc agcaggtgca gaccaccttt tttccgtgcc
     2821 agatcccatt cacggccgga acccaggtga tccagcagag agcc
//
LOCUS       DAWENO010000223         2805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11682_length_2805_cov_7.856727, whole genome shotgun sequence.
ACCESSION   DAWENO010000223 DAWENO010000000
VERSION     DAWENO010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11682_length_2805_cov_7.856727"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          686 a          803 c          734 g          582 t
ORIGIN      
        1 caagcagatt gcgtgcaaag ccgtcaggcg ggctttgtgc ggtattgcct ttattatacc
       61 gggttttccg gcggttgtca acgaaacggc agggtgtcaa atcgccgctc ttgacacatc
      121 cgcctccgcc atggtacaat atgggacaga accggacagc gccgcagtgc gccgtcccca
      181 cgaaaaaaag gagcagcaag ctatggcaaa acaacactgg aaaggcagcg tcctcttagc
      241 acccgtaccg ccgaccctgg tctcctgcgg cacgatggag catcccaacg tcctcaccat
      301 cgcctggaca ggcgttctgg cgacccatcc gcccatgacc tatatttccg tccgaccctc
      361 ccggtattcc tacgacctca tccgggaatc cggcgagttc gtcatcaatc tccccacctc
      421 cgccatgatg aaagccacgg atttctgcgg cgtgcgcagc ggcagagacg tggacaagtt
      481 cgcacagtgc cacctcaccc cagagcaggc gcagaccgta agtgcgccta tgatcgccga
      541 gtgtcccgtc agcctggagt gccgtgtcac cgacacgaaa gaactgggga cacacaccgt
      601 ctttgaggca gagatcaccg gcgtgaatgt ggacgagcgc tttctggaca gcaagggcaa
      661 gctgaatctc cagcagtgtg gtctcatggc atacgcccac ggggaatact tcgccctggg
      721 cagaaagctg gggaacttcg gcgattccgt ccggaaaaag aaaaagccgg gcaagggaaa
      781 aagacagccg gggaagaaag ggtgattttc tgtggggcag ggaaacagca ctgtaggggc
      841 gaatatagtt cgcccgctgt gcgactcgct gacataacga ctctgaccac acttcgtacc
      901 gtctcctcta tgagaggagg ctttataaaa attgatttat gtattgacaa atccagcgtt
      961 ctgtcgtata atatagataa agagagagca taccagcaga cggtctgctc ccaagaatta
     1021 gttatatgaa ataaccgcct ctagttggaa gctcagggcg gttatttccg ttttttgaag
     1081 tttaagtaag caagatatgc tttgaatact tcgcaaacca tgctaatgca cttgacaagg
     1141 atgtctatgt tcattcgcac tcactccctt ccgggagctg gaatcgaccg cctaccgtta
     1201 ttggtatgcc ctcagagctt tagtatatca catacgacaa aaaatgtcaa acaagcacgc
     1261 aataacgaga atgatttgtg tggaatcccc ctgcattcac cttgactttt ccacaaaatc
     1321 tgctataata tagaagtaga aggcagtgct gccttctttc tgtataacca cggcattccg
     1381 ggacaaacta acggggaaaa cacccgaacc gtccaaagga gtaccgctat gaccagacag
     1441 aacgacagca catccaaaag cagcaccgag gaacagctgg atcaggcaca gctgatcgaa
     1501 gagaccggcg gtgtccggcc gcagaacgcc aagcacctca tccactgtct gaacatcatc
     1561 ggacaggtag agggacacaa cgtcctgccg gaggacacca agaccaccaa gtatgaacac
     1621 gttctcccgg cgctggtcgc cattgagcag gatcgggaga tcgagggact gctcatcctg
     1681 ctcaacacta tgggcggcga tgtggaggct ggactcgcca ttgcggaaat gatcgccgga
     1741 atgcacacgc ccaccgtctc cctggtggtg ggcggcggtc actccatcgg cattcccctg
     1801 gcagtgagcg ccgatgtctc gtttattgtt ccctccgcct ccatgacggt acacccggtg
     1861 cgcaccaacg ggctggtgct gggtgtgccg cagactaccg tttattttga aaagatccag
     1921 gagcgcatca ccgggttcat caccgcccac agccgcatcc cacgggagac gctggaacgg
     1981 atgatgatga acacccagga aatggcgact gatatgggga ctgtgatctc cggcagagag
     2041 gctgtggaaa gcggtctcat cgaccggatg ggttctctcc acgatgccat cgaggcgctc
     2101 tatcatcaga tagaaacata agggcagcac tgcattcttg ccgtgccgcc gtgagaactg
     2161 caccccggcg ttctgccggg cagttccttt cagaaaggca cataacattg gcaaaaaaat
     2221 ctgcatcttc caaaaagact tccaagccag cacccaaaaa gaaaccggca gccaagaaaa
     2281 aggctgccgg tacttctgct gcacagacgg cgaaagaaga ggcactggaa aagctgaacc
     2341 gccagcagag cggcaacacc aagttccagt ccgtcatcct ctttgcctgc gcagtgctgt
     2401 ttttcctgct ggcgtttatc cacggcaatg ccggatggta ctttatgcac cgggtcatcc
     2461 ggggcatttt cggcttttcc atcgtcattg tccccattat cctgctcatc accgcctatt
     2521 acaccgaaaa gcgtgaggga atgtccctct cccggcggct gctgttcagc gccggactga
     2581 cggtactggt gagcagcttt ttccagatca tgttcatcgg tgacatcggg aaaaaaggct
     2641 ttttccgtgc catcggctac ttatacacca ccgccgcaga cgatgcattc cagagcggcg
     2701 gcgtcatcag cgccgttctc gcctatccct ttctgcggat catcggcacg cccggtgcgc
     2761 agatcatcat tgccctgctg ctctttgtcg ccattatgtg gatgc
//
LOCUS       DAWENO010000224         2794 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11725_length_2794_cov_7.957649, whole genome shotgun sequence.
ACCESSION   DAWENO010000224 DAWENO010000000
VERSION     DAWENO010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11725_length_2794_cov_7.957649"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1003..1012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          792 a          632 c          675 g          685 t
ORIGIN      
        1 tcattgacag agtgatattt gaaaaggcac aaaaccgata tgctaaaaga tgcagaaaaa
       61 agaaaagtaa aacaaaagat acgcttttca ctggatatgt atactgcaaa gattgcaaat
      121 ccagatttca tgtatttagg cataaagctc gtggcaacat gattgtaagt tatgagtgtt
      181 ctggttatag aaagcagcaa aaaaaatgca cctatcatcg tattgatgaa gatgcactaa
      241 agcaatgtgt attgcagaaa atcaaactta tgtggtacga agccaagagt gatattcggt
      301 catttaaagc caaagtaaga aaaaggctga atgccgatga aacagcatcc agaagcaaaa
      361 tccaatcaga aatagatgct gcacaaactc gaattgctga gattgataag tatgtacagg
      421 gtttgtttga agcaaaagtt cgtaacgaaa ttgattcaga tattttcgga agtttatcaa
      481 aaacatacac tgaagaaaag gctgaactga atgcaaaaat tgcagacctg attttacagg
      541 aaaatagtgc aaaagatgca tatcagaaaa tcaatagatt gtatgaagca attgaaaaat
      601 acgacagcat tacagagctt acttccgaag tcttatctga ttttgttgac cgtattgaag
      661 ttggcgaaca tttgaaaggc acaaaggaac agaaaattga cgtgtttttt gtaggtgttg
      721 gacttatgga cacgttatga aatcgtgttc cccatgcgaa ttacaactac atttatttac
      781 atttttcgac actctacata gaaaaatatt cttgctgatt atgtatattg cacaaaagta
      841 atcatatgca aagccttgtt atacacacta cagacaaatt cgagcaaaaa gagaaagtga
      901 tacctgcgtt ttctcaaata tccaaagacc aaatcaaaag ctaaagaagt acctttttgt
      961 tcattgccat gacgtcgcct cacgcatacc gtacaatccc ccnnnnnnnn nngattcgat
     1021 gtttttgcaa tttttccagc agctcttctg tttttttctc cacgtgggcg aaaccggctc
     1081 gttccgcaag cccctgcgcc gggaggagga gcaagcgtgt ttcgaggcgt tggcggcagg
     1141 cgaccgcagc gcccgggaaa agctgatcct gcacaatatg cggctggtgg cgcacgtcat
     1201 caaaaaatac gggatgcccc agccggaaca ggaggaactg atttccatcg gcacggtcgg
     1261 actggtaaag gcagtggact ccttcgactg caccaaaggt gcaaggttcg ccacctacgc
     1321 cagcaagtgc atcaacaatg agatcctgat gaacttccgg agccagaaaa aacacgccga
     1381 ccagatctac atgggcgaac ccatcgagac ggacggcgac ggcaaccagc tgacgctgat
     1441 ggacattatc gacgacggca cgaacctgcc ggagatggtg gatacgctga tccagtcgaa
     1501 acagctgtac caatatctgg aacgctgtct gaccccacgg gagacggaga tcctggtgca
     1561 tcgctacgga ctgtacggct gcgcacccat gacccagcgt gaagtggcga aacagctggg
     1621 catttcccgg tcgtacattt cccggctgga aaagcacgcc attgaaaagc tgcgccaggc
     1681 gtatcaaggg caataacgca aaaaagccgt gaacggattc gctcacggct ttcggtttca
     1741 attttgggca acaccgcaca aagcttgtgc ggaagatgcg tagccagatt tttcgacaga
     1801 ttgcatagaa attccgcagg catactgtcg tatggcaagg aatttctggg caatatgacg
     1861 gaaaatcagc aagcagattg cgtacaaagc cgccaggcgg gctttgtgcg gtgttgcctt
     1921 tggtcgggat tatgcgctgt acggcagggt gtccacgata tgcaccagga attgcagcag
     1981 aacaactgca tcgccgctgc cgggttcgcc gtcgccgttg cagtcggcgt ttttctttgc
     2041 ggtatcatcc agcgtgatgg cgccggagca gaacttgttc agcagaacag cgtctgtgat
     2101 gtccactcgc ccatccaggt tgatgtcgcc gcagacgaga cctccagtga ttggtgcggc
     2161 ggtggttgtg gtggtttctg tggtggaagt ggttgttgta gtcgtcaccg gtggcgtgtc
     2221 agagtcatcc agagccacaa aggggaagcc gaacatttcc gcataccgct cggcgtaggt
     2281 gtccttgtag ccgtacatgg tcagcttggt atcataggta aacgccatgt aatggatacc
     2341 cgtcacgctt ttcggaatga cgatcttttc cagattgtcg cagttggcaa aggcatcatc
     2401 ttcgatcagc gtaatgcctt cttccagtgt gacctctttc agccgtttcc cgtctttata
     2461 agcaccggca ggaattttgc cctctttgtg cttgacagtc agctgggtaa catttgtagc
     2521 aacactgaca acttctccct cttgaccgta ggtatcttct gtggtaaatt tgatatccgc
     2581 atcacaattg gaaaatgctc cctctttgat acccgtttta gagcctaaaa atgtaataga
     2641 cttcagattg gtacagtcct taaaagcttc ctctccgata tacttcacgc tgtccgagat
     2701 cgtgatttcc gtcaaaccgg tacagccgga aaaaacacac tctccgatac tcgtcacgct
     2761 gtccgggatc gtgatttccg tcagactggt acag
//
LOCUS       DAWENO010000225         2790 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11735_length_2790_cov_12.616088, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000225 DAWENO010000000
VERSION     DAWENO010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11735_length_2790_cov_12.616088"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          643 a          725 c          732 g          690 t
ORIGIN      
        1 ctataccaac tataactgct atcagttcgt gggacaagat gtccgggtgc tgaaacgcca
       61 gcagcagctg cagcatggaa acggataagt ggctgttggg tcgtaaagga aaattggcgg
      121 tgaaaatgaa aaaatcccct tgacaaaagg ggcgcaatat cgtataatag aaatagaaag
      181 agagcatacc agtagacggt ttgctcccac tgctttgatt aaaaaagact taaccgccgc
      241 aagttgggga acttttacgg gcggttattt ctttttattc tcgtcattgc ggtgatctgc
      301 attactattt ttatgattat agtaaatgta aaagatcaga cttgcgagat ttagcagaat
      361 actgcaaatt ttcagcaagt cgtcaaaagt gacgtactga tccatcatag caatcacctc
      421 ccaactgttg taaattcagc agtgggagca gaagcaaaag aattttcaaa ccgcctaccg
      481 tttcaggtat gccctcagat atctattata ccagacacct cgaaatttgt caaaccccaa
      541 aagcacccca accaaaggag catccacatg gaaaaactct tactgatcgc cattcccgac
      601 accgccggag tggaacaaat cgccggaaag ctgcggctgc ggacggtgac tgttgcgccg
      661 gagaactacg gcaaaagcct gggcagtctt gccggctatg ccgccggaac ggttccggtg
      721 tccgctgaaa ccaccctgcc ggaaaccggg ctgctggtga tgtgcggtct gaaaaattcc
      781 cggatggaca agctgctggc tgccctgcgc accgctggta tccgcattcc ccacaaggca
      841 gtccttacgc caaccaacca gaactggaac gtctatcagc tggcgcagga gctggaacgg
      901 gagcgccagg caatggaaaa ccgctgacct gcgaatgcaa atgcaggtaa cgtcataata
      961 caaaaaatca cggcataccg caatcgggtg tgccgtgatt tttctattat gaaagggaaa
     1021 aatgttattc gcccagcagg aacttcgtca ttgtcacaac gtcctggatg ttgaactcgt
     1081 tgtcagcatt catgtcaacg ctggaatcca gaatcagatt ccgatcatct gcggatgcgt
     1141 tcagcagata ctgccgcatc agcgccaggt caaagccgtt cagcataccg tcgtggttca
     1201 catcgccacg gatcacgtca tctcccggtg tggttccggt ggtgactgtt gtggtttctg
     1261 tggttgtctc cgaagtagtg gcagaagtgc ttgtggtagt taccaccggc tccttgccct
     1321 ctacgatgga gtagaacgcc ggcttagcgg tgtagtcctc gttgaacagc agcggagact
     1381 ttgcagctct ccagctcttg tcgtcggtag taccccagaa taccactgcg gaaatgctgt
     1441 cagcgtattc cacgcaggca tccatgatgc ccttgtacat ctcagcctgc ttttcaaagc
     1501 cagcctcgga agtgtcagaa gtggtgatgt ccagctcggt gacctggata tccagaccag
     1561 tctctgcgaa cttcgccagt gcctttttat acgcagaaac ggtggggaaa ccaacgtcca
     1621 ggtgagactg cataccgata ccgtcgatca gtcccttttc cttcagttcc agtgccatct
     1681 tgcagatcgc atcggttttc tgcggcatat attcgttgaa atcgttgtaa tacagcttgg
     1741 tgttcttcgg tgcatacttt ctggcatact caaatgccgg ctcaatgaag gagttgtcgc
     1801 cgaatacctt gacccatccg gaatagttgg cattctcgga ctgtccgccg ccggtacggg
     1861 gtgagccgtt gtccagccat gcctcattga ccacatccca tgcgtagaaa tctacggtcg
     1921 ggtactcttc ttccagtgca gcgaaaacgt tcttgatgta gttttccata cgctggagca
     1981 tgatatcctt ggagacccag tcgccgctgt cctggaagcc ttccttgaag aaccagtccg
     2041 gtgtctggct gtgccatacc agaacgtggc cacgtaccgg gatattgttg tcccggcagt
     2101 agttcaggat ggttctggcg cttgccagat tgatgaccgg ttcggtctcg ctgccggatg
     2161 cctgggttgc gctcttatcc aaaatggatt ccggtttcag ctcgttgccc agggtgatgc
     2221 tgttgaagtg cttggcgatc agatccttgg tggactgggg tgcaagctct tcggtggtga
     2281 cagccgtgcc gatcttgaaa tcgttggcat aaacatcctt cagagacgga atatccttct
     2341 ggatctgata taccggcgct gtgcgcacct cgaaatcgtc gatatacatg gttgcggtgt
     2401 cgttgcactc aaagtacaga tagaccttgg acacatcctc ggaaaagctg aacttgatgc
     2461 cggagaaggt tgcccagtcg ccgttgctgg tctgcgcctg caggttgctg tagtgacgct
     2521 cgccgctggc atcggtgtat tccatgctca gcttgatgga gttataccac tcggtctttg
     2581 ccacagcgct gacggtgtac tgcaccccag cctcgcactt gtcgtccagc aggaactgcg
     2641 gaccgttcca gttcttggct ctgtctttga cgcacatgga ataagagccg ctgtatgcct
     2701 gttcggtggt ggcggtgatg gtttccacgc cgcctctgcc ggtgaatgcc gaaacatcgc
     2761 cgtcctcgaa tccggtgccg tagatctggg
//
LOCUS       DAWENO010000226         2787 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11746_length_2787_cov_8.078697, whole genome shotgun sequence.
ACCESSION   DAWENO010000226 DAWENO010000000
VERSION     DAWENO010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_2787_cov_8.078697"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a          503 c          634 g          916 t
ORIGIN      
        1 gaaaagatat ggaatccttg tttttcggaa atacaaaaca ggtatatcaa aaagaagaaa
       61 catcagaaag tatgtatatt cccgggaagg actactatca tttatatcag acacaggata
      121 ataccacgct gtgttatgat gcaggtagca ttctgtacgc cacggaaaaa tccgatgact
      181 actatggcta ttttgcatat tattatgacc tgtcctgttc agaagaaaac atgcgtgtcc
      241 tgatgccaga aaaagaattg gatacatttt cctctacgga tgctcttgca tatatgcaaa
      301 atctgataga aacgttagat ttacctgtgg caagcacacc agagatatat gcgctcaatc
      361 agaattgcgt cgagaatgca aaaacaaatg ttggtgtgga tattccgtgg gatgacgagg
      421 cggatgcgta tatgcttgtg tacctaacgg aaatagacag cgttgctgct gccaattata
      481 caatttcttc ctctgtcagc gatatgaccg aaggacgccc gtcaaagctg gtcagcgtgt
      541 tcagcaaaaa cgggctggaa tatctggaat gcagttatgc aatggaacaa acagaggaag
      601 gggacagcag tcagatctgt tctccggaac aggcaatcga acgagtgaaa tcccatatgg
      661 aatctatggc tgtgaatgaa gatgaatcaa aaggtacaga aacactgtcc gggtgcagtt
      721 tgaagtggaa tgttctggaa caaaaagagg acgtactgca attacagcct gtctgggtat
      781 ttattttgga ttctgtgctg cccaataatg atgggacttc tacacattat tacagtacga
      841 tttgtgtgga tgcactgact ggtgagttgt attagtgagg tgctgtatgc agatatttat
      901 ttcttacatc aaacgattac ttttgtcgcc tcttttttat ctggcaattg tcatggtcgg
      961 cgttatctgc tggtttggta tgcaggagga gaacgcacgc cccggcgtga tctatgcgtt
     1021 cgatctcatg ttggacatca gctcttaccg aaaattgatg atgctgtttg caacgattcc
     1081 gtttggaacg gtttattgca gagaacgaaa cacaaaaatg gcaaatttta ttttgtatcg
     1141 ttcctctttg tcaaagcatt tgtcagttta tctggtaatg cagtttgtat cttcttttct
     1201 cattgtgttt ttgggaatct tattgtgcat cgggcttttt tgcatacgag gcacactgta
     1261 ttgttatgat ggaaatgatt ataccggaac atttggcgta cttctcaatg aaaaagatgt
     1321 ggggctgtat ttgtttttcc ggacgtttca ttatgcctac agtattgctg tgtggtcgct
     1381 ttccggtctg gcaatgtctg cctgcttttt gaatgcgtat ctggcagtct gcgctccgct
     1441 tgttttcagt tatgtgttgg agatgattac aattgaatca aagttcctgc cgaatttatg
     1501 gtatctgtct ttgagctaca cagatgtttc ctctaatccg tggctggcat ctgcttatat
     1561 tattcttgta ttttcatgcc ttggcatagt gttcgcattt gttttttggc aaattgcaaa
     1621 aaggagggtg caggatgtca ttcattaaaa taaccgtttg catttgtttg cagaacttca
     1681 gaaaatggca aaaggactat cgtatttgga cgattgccgc aatcctgttt atcttgattc
     1741 tggaaaatac gagaaaattt tccgatttga gtgctgattt aggcgtttcg tcttctatat
     1801 ggcagtatcc gtttctgtat tcgcaatatc atctgaaatt gatttttacg ctgccgctgc
     1861 ttttgatgtt ttgcaatgca ccttttatcg atcaaaacgc attatttctt attgtaaggt
     1921 gtaagcgaaa ggcatggatt accgggcaaa tcctatacat tatcgtatcc agtgcggttt
     1981 actatctgtt tatctttttg tgtactgtga ttaccgcact tccttcatcg gaactttcta
     2041 tggattgggg gaaatgcctc tatacaattg catacagtaa tgcggcatca caattgggct
     2101 attcgttttt gtctgtcgcc ggttacgtca ttgaatattt tacacccata caggcggttt
     2161 ggtatacgtt tttactatca tggctcatgg cgatattcat tggattattg atttactttt
     2221 tgaacaccat cagcagtaaa cgctacttgg ggagcatcgt cagtggaatt attatcttat
     2281 tcagctgcta tgtggaggca ttcggttctg ataagcagtt gtatttttct ccggtttcct
     2341 ggagtacgct gaatcaggtc gatgttgctg aaaagacaaa tcatccttcc ttctattatt
     2401 gcatcggcgt ttattgcggt ctttctttgt tattgattgt tttgctgtta gtatttagaa
     2461 agagatggaa aatggatatt acgtgattca agttacagat cttgtcaaat catttggaaa
     2521 agaaaccgtg ctgcatcata ttaacatgaa ctttgcgccc ggaaaaatac atggtatcat
     2581 cggacgaaac ggcagcggta aaaccatgct gatgaaatgt atctgcggat ttattccggt
     2641 tacctccggg aaagtcactg tatttggaaa agtgatcgga aaagacgttg atatcccgga
     2701 taatataggg gctatcatcg aaacacctgg atttcttccg gagtatggtg cttatcggaa
     2761 tttgaagttt ttagcggaca ttaccgg
//
LOCUS       DAWENO010000227         2782 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11761_length_2782_cov_12.331867, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000227 DAWENO010000000
VERSION     DAWENO010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11761_length_2782_cov_12.331867"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2297..2306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          622 a          710 c          778 g          662 t
ORIGIN      
        1 gcaagccgca ggcttgacgg aggagtctcg ggcgaacaat gttcgcccct acggtttctc
       61 ttttcaaaaa gggtggacaa ctcctctgcc ctgccgggca tttcctctgg ggaaggagac
      121 tttgggaatg tttcacgtga aacattgcgc cgctggtgcg gttcagaaca gctccgggtg
      181 atgtttgacg ctgtcctgtt ccgtgatctg acggatgaca cggcaggggc tgcctgctgc
      241 cagggagttc gccgggatgt ctctggtcac cacgctgcct gcgccgatga cacagccgtc
      301 tccgatggtc acgccgccgc acacggtgac attgctgccg aaccagcagt tatttcctac
      361 ggtaatgggc ttgccgtatt ccaggtcgta gtacgagccg tcgggacgct gtttcagatt
      421 acgctcctcc gggagatagg cgtgcatggg agagacaaag gagcagttcg gtccgataaa
      481 tacgtcgtcc ccgaatacca ccgggcagac atccagaacc accaggttga aatttgcata
      541 gcagcgcttg ccgaatttgg tatagcagcc atagtcgaac tggagcggtc cctgcaaaaa
      601 agtgccttcg ccgcagtcgg ggagcagttc cgccatgatt gccttgcgtt tttccgcatc
      661 cgtctccagg gtgtcgttgt attccttgga cagccggtgc gccagctgcc gccgtttttc
      721 cagcctggaa tcgctggggt cgtaaagctt tccggcgagc attttctgtt cttcggtcat
      781 aggggttacc tcctttttat tctgcgcaga aatgtgaact gtatacagtt ccgtgcgttt
      841 tgatacctct attatagcag aaacagggga caggtgcaac tacaatttct gctttttcct
      901 gcactttttc tgcatggagt atccaggctc tcacaatggt gcggaaaata tacgatattg
      961 atagtagtgc agacgattga tatatatttt tgcttgcatt tcgtctaaat ttgtgctatg
     1021 ataatatcaa tcaaatacgc accccggtgc tggaaaggaa acctgcatta tgttaaatat
     1081 tccgaaaatg aaagtcggca ttgtcgcagt cagccgtgac tgctttccgg agagcctgtc
     1141 ggtaaaccgc cgcaaggcgc tggtggatgc ctatgccaaa aagtataatg ccgaggatat
     1201 ttacgaatgt cccatctgca ttgtggaaag cgagatccac atggtacagg cgctggacga
     1261 tctgaaaaaa gccggctgca atgcgctgtg cgtctatctg ggcaacttcg gtccggagat
     1321 cgccgagaca ctgctggcaa agcattttga cggtccgaag atgttctgtg ccgctgccga
     1381 ggaaaacacc ggcgtgctgt cctccagccg tggggatgcc tactgcggta tgctcaacgc
     1441 cagctataat ctgaaactgc gtggcgtaaa ggcatacatt ccggaatatc cggtgggaac
     1501 tgccagcgaa tgcgccgata tgatccacga tttcctgccc attgcacggg ctgtctacgg
     1561 gctgaaccat ctgaagatca tcactttcgg ccctcgtccc accaatttcc ttgcctgcaa
     1621 tgcgcccatc cagcagctgt acaacctggg tgtcgagatc gaggaaaact ccgagctgga
     1681 tctgttcgag gcgttccaca agcacgacaa cgacccacgc atccccgaag ttgcggcaga
     1741 tatggcggca gagctgggca gcggcaacaa aaagccggag atcctgccga agctggcgca
     1801 gtatgagctg acgctgctgg actgggtcga ggaacacaag ggctaccggg aattcgttgc
     1861 tattgcaggg aagtgctggc cggcattcca gacccagttc ggctttgtgc catgctatgt
     1921 gaacagccgt ctcaccggac ggggcattcc ggtttcctgc gaggtggaca tttacggcgc
     1981 actgagcgag tttatcggca cggtggtcag cgaggacgct gtgacactgc tggacatcaa
     2041 caacaccgtt ccggcagacc tgtacgagaa tgagatcgcc ggaaagttcc cgtaccgtct
     2101 gaatgatacc tttatgggat tccactgcgg caacaccacc accaaaaagc tggcgttctg
     2161 cgagatgaag tatcagctta tcatggcgag atcacttcct atcgaggtta caaatggaac
     2221 actcgaaggg gatatcgtac cgggtgatat cacattctat cgtttacaga gcacagcaga
     2281 tactaagctt cgcgcannnn nnnnnncgga gtctgccgga agaggtcaca cagggtacgc
     2341 tggaagggga cattatgccg ggagacatca ccttcttccg tctccagtcc acttcggatg
     2401 ccaagctgaa cgcctatgtg gcgcagggcg aggtgctgcc ggcggcaacg cattcgtttg
     2461 gttcgatcgg cattttcgcc attccggaaa tggcacggtt ttatcgccat gtgctggtag
     2521 agcatcattt tccgcaccac ggcgcagtgg cgtttggcca ctacggcaag gcgatctatg
     2581 aggtgttcaa gtatctgggt gtggatgtga gcgagatcgg gtataaccag ccgaagggtg
     2641 tacggtatcc gacggagaat ccgttttgct gatacagctg tgaaaacact gccagtcgtg
     2701 tgatacgctg ccgattggaa taagggtagg cgagagcttt tctttttttg cttctttttt
     2761 tctttttagc aaagaaaaaa ag
//
LOCUS       DAWENO010000228         2778 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11782_length_2778_cov_7.789203, whole genome shotgun sequence.
ACCESSION   DAWENO010000228 DAWENO010000000
VERSION     DAWENO010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11782_length_2778_cov_7.789203"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          579 a          813 c          741 g          645 t
ORIGIN      
        1 ggagacagcg gatcagatcc agatgagaaa tatattttgc ccgtccggtt ttcataaacc
       61 gcattctgac gatttctttc aaaagcaagc cctccctact ctccggttga cgccgcagcc
      121 ggaacacttc gcccggcagt gcggcgtggt ctcagatgcg tgcgccgtct ggttctcacg
      181 cacgaaaaac gccttgtcca ccagataatc caggtgatcc cacggaaaga tctcatcata
      241 ggctctggtg cggttggcgt agaatgccgg gtctgcgccg cattcctgca tcgcctgctc
      301 ccagatctca tagtggaagc attcgctcca gctgtcgaat ttgctgccca gctgccaggc
      361 acgttcgatc accggagcga gccgccggtc acctctggcg aggactgcct ccagcaggct
      421 gatctcatag gtgtgccagc tgaccttgat cttcttccgg tgccgtggct ggatacagct
      481 gagcagatgc tcctggcgtt tcagcagttc ttcccgggag acctggggtt cgaattcaaa
      541 cggcgtgaac ggctttggca caaaggtcgc acagctaatg gagatctgaa tggtctttcc
      601 tttggagcgc tccggcgtgt tatagtagag gtcgatcagc ttttctgcca gatgcacaat
      661 gccctcgata tcctccagcg tctctgtggg cagtcccatc atgaagtaga gctttaccgt
      721 cgcaaaaccg ccagaaaagg caatgcctgc ggtgcgcatc agttcctctt cgctgacatt
      781 tttgttgatg acatcccgga gccgctgtgt gcctgcctcg ggtgcaaagg taaggctgct
      841 cttccgcaca ccctggatgc gttccatcag ttcccgggag aagttgtcca cccgaagcga
      901 gggcagcgtg atgttgatgc ggttttcatc cagatacggc agcagttcgt tcagcgtttc
      961 ttccaggcgg ctgtggtcgc tggtactcag cgaggacagg gagagttcct ggtagccggt
     1021 ggtgtcacag agcgagcggc actgccgcac aatggttttc ggggattttt cccggaacgg
     1081 acggtagagg aaacctgcct ggcaaaagcg gcagccacgg atacagccac gcagtacttc
     1141 ttctacggca cggtcatgta cagtctccat gaaaggtact acaaacgtct ccggataaaa
     1201 cgccttgtcc aagtcggcga tgatgcgctt ttccaccttc gccggagcgc cgtcttttgg
     1261 cgtgactgcc gaaacagtac cgtccgggtg gtaggtcacg tcgtacagcg acggcacata
     1321 gatgcccttg atctgcgccg ccgcacggag aaattccgaa cgggatgccc cacgctgttt
     1381 cagttccaga tagagatcca tcagttccag gttgacttct tcgccctcgc ccagaataaa
     1441 caggtcaaag aaatctgcca gcggttccgg attacagacg cacgggccgc ctgccaccac
     1501 cagcggcgca agcgtttctc cacggtcacg ggcgtgcagc ggtattccag ccagatccag
     1561 catattcaga atattggtaa aggaaagctc gtattgcagg gtaaatccca caaaatcaaa
     1621 gtcccggaca gcatccaggc tttccagggc atacagggga atctcgtttt gccgcatcaa
     1681 tgcctcaaag tcggcgcagg gcgcaaatac ccgttcgcac cagaaatttt ctctcgcatt
     1741 gatgagacta tataggatct tcatgcccag atgagacatt ccgatctcat aggtatccgg
     1801 aaagcaaaag gcaaagcgga catccacatt ctcccgcttt ttgacaacgc tccccatttc
     1861 accgccgata tactgcgccg gtttctgcgc ctgcagcaac agcggttcga ttttcgataa
     1921 tacattcatg ttttttcgtc cttatgattt gatgtactcc tattataccg gattatgccg
     1981 aaaaatgcaa gtgtgagaaa cacaaaaaaa gaggacgcag cgtcctcctt tcccgttttg
     2041 tctacgacag cagttcctgc cgcagttgtg tcaatagttt tttctcccgg cgtgagacct
     2101 gcacctgcgt catgcccagg atggctgcgg tcttgctctg ggtcagttcc cgaaaatagc
     2161 gcagctccag gagcatccgg tctttttccg gcagtgcgcc cataagctgc cgcagtgaca
     2221 gcaggtcacc gatctgcctg tccggtgcct ctaccgggat gtcgaactgt ccgtcctcct
     2281 cgtcgtcctc gtttcgggtg agggaaagcg gcgtctgtgt gacgcacagc gcctcgccgc
     2341 acgcctcgat ggattcaccg ctgcgctcgc tgagttcggt catggtgggc gatctgccgt
     2401 agcgctgttc aaactcttcc tggagctttt gcagtttcat ggcacgttcc cggaggctgc
     2461 ggctcacgtg gacaatgccg ccgtcccgga acaggcgctt gatctcaccc aggatcaccg
     2521 ggacagcata agtggaaaac tgcaccccac ggccgcagtc aaaagccttg atcgctttca
     2581 gcagtcccac acagccggca gagtacaatt cgtcatactc gattccccgg ttgcggaaac
     2641 gattggcgca gagatgcacc agtcccagat gctcttcggc acggggtgcg gtcacttcac
     2701 ccggcatggc aaagctccca gctttttccg catggtgatc gtggtgcctt ttcccggcac
     2761 gcttttcacc ttcagctt
//
LOCUS       DAWENO010000229         2772 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11808_length_2772_cov_11.979389, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000229 DAWENO010000000
VERSION     DAWENO010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11808_length_2772_cov_11.979389"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          647 a          666 c          775 g          684 t
ORIGIN      
        1 cacccgaaca atggatggtt tagtgaagtt ggaataatat cgttgcactt tttgaaactt
       61 ccatattttt gatacgaata agataaaaga ataaattata tcattctgca ttttcgaaaa
      121 tgaattgata atgcaacaaa aaacggtatg gcagaataaa attgccatac cgttttgctg
      181 taccgccggg aaaacgccgg acatatttag tctgcggaag gaacgctgcc gtccacttcg
      241 atctcgacat cgccctgtgt gtcgccgtcg gacttcacca taacagagtt gctgttctct
      301 tcataggtga actcgttgcc gttcttgtct tttaccttat atgtcagatc gtcaccgtcc
      361 tggctgacca ggtcggcgtc atagtcctgt ttctccccgt tgatatagag ggagagcttg
      421 gagaagatct cgccgccggt ggctgcgttg gcggtcacca gggagactgc gccgacagca
      481 acagcggctg cggcaaagat gatcggacga atgcggcgga gtttggattt ctgtttcatg
      541 gggagtacct cactttctgt gggctgagaa gattcggcga tccggcgcaa gacacgcttt
      601 tcgacctgtt ctgccatctg cgtttcttct ttcagctgta acggcggata ttgtgcaaac
      661 caatcgtttt tattctgcat ggtcaaaacc cctttcgatc atatatttcc ggagttttcc
      721 tctgcttcgg tggagtctga gccggacagc agtgtcggtc agaccgcagg cttttgccaa
      781 cgctgaggtc ttttctccat agaagtaaaa gcgtatcacc agagagcggt ccggctcttc
      841 cagcttgtcc agcgcccgga tgaggcaggc gattgcctcg gtggtctctg catcgccgca
      901 gaggtcgctt ttgtcggaca ggagcaggtc ttcataagac tgccggacag gcggctgctt
      961 tccctgggtg cggaaatagt tcttcatgca gtttcgtgca atggctgcta aataagggga
     1021 aagatcggcg ccgtccttca gcttggcccg gctacgccag agccggagga atacatctgc
     1081 ggaaagctcc tccagatcct ccggcggcag ggtgctgcgg gagaaattgc ggaggatggt
     1141 gctgacatag ccggtgcgct gccggatgat ctcgcacaat gcgtcctcgt cgccctgccg
     1201 caggcgtgcg ctccaatttt gttccatggg atcacctcgt ttctgaacgt tcgcttatat
     1261 atacaccgct tttggaaaaa tgtttcatgg tgaggactgg ggatttcact ttataataga
     1321 attggctggg caaaaaaaat tttttccatt tcctattgac aactgctttt ggatatggta
     1381 tactatacgt gttgcatgag aagtacaaca cttacaatac atacaacgca gtggatgcag
     1441 cgtggtgttt gtgtaaaaat agaatggagg ttttgccatg ttccagatcg acccgatgtc
     1501 aagagaaccg gtctatgaac agatcatcca gcaggtgcag cagttcattc tgacggatgt
     1561 gctgcagccg ggcggtcaga tcccgtctgt gcggagcgtt tcgatgacca attccatcaa
     1621 tccccggacg atcttaaagg cgtatacaga gttggatgcc aaaggactga tacagtccgt
     1681 gccgggaaaa ggatattatg tctgtgcgga tgcaaagcaa aagctgttgg agaaagggcg
     1741 agagaagatg aaagatctga cggagatgct gacggcgctg gcgatggcca gcgtgacgaa
     1801 agaggagatc accgcctgtg tggaacaggc gtttactgct gcaaaggaac agcagaaggg
     1861 aggagaatga gtatgatcga agtcaaaaac ctgaccaagc gctttcatga ttttacggcg
     1921 ctggaggatg tctcctgttc catcggttcg ggctgcatct atggcatggt cggttcgaac
     1981 ggcgccggaa agtctacgtt cctgcgtgcg ctcaccggcg tataccagcc ggatgccggc
     2041 aaaattctca ttgacggtca gccggtgtgg gataatcctg ctgtaaagcg gcgcatcgcc
     2101 tatgtgccgg atgagctgta ctttctcgcc ggggcgaatc tgaaccggat ggaacggatg
     2161 tatcagagtc tgtaccccga ctttgataaa aatcgtttcg aaaagctgtg cggtcattta
     2221 cagctgcccc ggaacaaatc cctgggacag ttctccaagg gaatgcgccg ccaggcggcg
     2281 accatcctgg cgctgtcctg ccgcccggac tatctgttct tcgacgagac cttcgacggt
     2341 ctggatccgg tgatgcgcag ctttgtgaaa aagctgatct gcgaggacgt gctggagcgt
     2401 ggcgcatctg ccattattac ctcccactcc ctgcgggaac tggaggatat ctgcgaccag
     2461 ctggcactgc tgcacaaagg cggtctggtg ctggaaagcg acatcgagaa tctgaaaact
     2521 tcacagttca agatccagat cgctttccgg gaggactatg acaagagcaa gtttgacggc
     2581 atccagatct cccactacga gaagagcggc agcgtcgcca atctgatcgt cagcggtgaa
     2641 cgggaagata tcgtgcagaa actccgtgct atggatccga tcctgctgga tgtgctgccg
     2701 ctgtcgctgg aagaggtctt tacttatgaa atggagacgc tgggctatac gttccagatc
     2761 gatgatttaa ta
//
LOCUS       DAWENO010000230         2730 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_11996_length_2730_cov_8.781308, whole genome shotgun sequence.
ACCESSION   DAWENO010000230 DAWENO010000000
VERSION     DAWENO010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11996_length_2730_cov_8.781308"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    566..575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          573 a          789 c          642 g          716 t
ORIGIN      
        1 ttgtccccga attggagcgt gtcaccgtcc tccaccgttt tccattcctg aacaggctga
       61 aacggctggg tagacagcca gtcgcccaga ttggcacggc tgtcagtcag catatttgca
      121 tcaagagaat gcacatagac cggaatctga tacttttccc gtacctgctc cacaccggca
      181 atgtgatcgt aatgcccgtg cgtcagcaga attgccaggg gttccagctg catcatttcc
      241 agacggcgga acacgatagg cgcatcaccg ccgatgtcca cgatgacaca cttcttgttg
      301 cccacttcct ggatataaca gttcgatgcc agttctccca gcatcagcgt tgtaattttc
      361 attgcagcca cctcactttt ttgcacgttc cacagagatc acgccccgga tcttccgcag
      421 cttatccatg attgcctgga gctgatctgt tccggcaacc tggacgctcg cctcaaaaat
      481 cgcattgccg ttgcgcagat tccgggatga cgaatgcacg atcagaatat gcgattccga
      541 gagaatcttc gtaatatcaa acaccnnnnn nnnnnagccc cacacggtct gacgcaatca
      601 cttcgatgcc ggtaggaatc agcgcagaag tgttgttcgt ccactgcacc ggcagccagc
      661 gtgccatttc ctccgggttg ttccgtttca gcgccgcctg gtagttgacg cagtttttcg
      721 tatgcacgga aatgccgtgt ccccgtgtga taaagcccac gatatcatcg cccggcagcg
      781 gattgcagca ctgcgcaaac ttgaatacgc agtcgtcgat ccgatccagg acaatacagt
      841 ccttaccgga tggtgtctcc gtctgcatca ccggtgcaac cggatttgtc tcttcttcct
      901 ctgcgccgtg cgatttcaca tacagttctt tcagtctcgg catgatcttg gacagcataa
      961 cgccgccgta tccgatcgag gcgtagaagt cctccagcgt gtcgcagttg tgccgtttca
     1021 gatcatctgc caggaatgcg ccccattccg tttccggcag attgatgtga cagcgcttga
     1081 actcctgctc cagattcgcc ttgccggtag caatgttctc ttctcggcac tctttcttga
     1141 accaggaacg aatcttggac tttgcctcgt tggtcttgac aatacccagc catgcacgat
     1201 tgggtccctt gttggggtct ttgctggtca agacttcgca gatctgtccc gtctggagcg
     1261 gataatccag cggaaccatc ttgccgtcca ccttgccgcc gatggtcttg tgtccgattt
     1321 ccgtgtggat gcggtatgca aagtcgatca ccgttgaccc aaccggcaga gagatggaat
     1381 cgccacgggg cgtcattacc acaatatcct ccggagacag gtcgttcttg atggcacgga
     1441 caatttcttc cacgtcgtcc gaggtctgct gcgcctcaat taccttgcgc acccatgcca
     1501 ggcgctcttc catcttatcc ttgccctgga ttccctcttt gtacttccag tgggcagcga
     1561 tgccgtattc cgcagagacg tgcatatccc aggtgcgaat ctgaatctca aaaggaatgc
     1621 tctcccgtcc cagaacagta gtgtgcaggg actgatacat attggacttc ggcgtagaga
     1681 tatagtcctt gaaccggttc ggcaagggtc ggaacatatc atgtacaata cccagcacca
     1741 tatagcactc tgcaaccgta gagacaatga cacggacagc atacttgtca tagatctcat
     1801 ccatgccctt gtggttgaca tagaccttct tgtaaatgcc gtagatgctc ttgacacgtc
     1861 cctcaatgat cggttctttc gcaaagtgtt cctgtgccag gcgtttccgg atacgttcct
     1921 taatggattc gatgaacttt tcccgttcat cctttttgat ctccataata tgttcgattt
     1981 ccgaatacgc ataggagtcc aggtaataga acgccaggtc ttccagctcg tcctttacag
     2041 tacggattcc cagacggtgt gcgatcggcg catagacatt catggtttcc agcgcaatgc
     2101 ggcgctgttt ttccggcggc aggaatttca gtgtacgcat attgtgaata cggtctgcca
     2161 gcttgataat catgacccgg atgtcatggg acatggcaag cagaatcttc cggacattct
     2221 ccgcctgctg ctgttccttg tcgaaaatcg gaatgcggtt cagcttggtg acgccgtcca
     2281 ccagcatcgc cacatcctgt ccgaacttct tccggatatc atccagcgta tattccgtat
     2341 cctctaccac atcgtggagc agacccacgc agatcgtatc cgtatccatt cccagatcca
     2401 gcagaatatc tgccaccgcc agcggatgga taatatacgg ttctccggaa gagcgtttct
     2461 gcccggcgtg tgccttttct gccagctgat acgcagacag gatcttgctc agatcgtact
     2521 gtttatccgt cgcaagaatc ttcttcgcca gatccgagat cgtgacctca ttctgaggga
     2581 tctgcggttt atcctgtgca gccaaacgca catctccgct tgtgttttcc tgtgtcatac
     2641 aacatccgct ccttttttct gtaatccctg cagcaggacg gaactttgca gattcacctt
     2701 ttcctttacc ggattccggc gtacccgctg
//
LOCUS       DAWENO010000231         2711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_12087_length_2711_cov_9.752259, whole genome shotgun sequence.
ACCESSION   DAWENO010000231 DAWENO010000000
VERSION     DAWENO010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12087_length_2711_cov_9.752259"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          582 a          721 c          704 g          704 t
ORIGIN      
        1 tcagcttgtc cataaagctt tccatgacgg caaagcccag cccggcacgc tcttcctccg
       61 gcgcagtggt aaacagcggc tgcatcgcct gtgccacgtc tgtgatgccg cagccgtggt
      121 ctttgatgcg cagatagatc tccctgtctt ccaggagctt gatatgtaat tccacatcgc
      181 cgctgcactg gcggtagccg tggacaatgg cattggtcac cgcctcagaa actgccgtgc
      241 ggatgtccgc cagttcttct accgtgggat ccagctgtac cagaaatgcg gagaccacgg
      301 ctcgggcggt tcgctcattc tcggagatgc cggggaaaat aaacttcact gcgttgatga
      361 tcttcactgt tcgtccctcc tctctgccgg ttcttccggc tgtgctgccg gggcatccat
      421 ctgaatccgt gcaatgcgtt ccataccgga aagccgcatc acccggcgga tatgctgcgg
      481 cggattccgc acaattactt cgccgccgat ggattccatg gagcggtgcc gtcccataat
      541 gagtccgatg ccggagctgt ccataaatgt aacgcctgca aaatccagca ccagcacttc
      601 cggctgatat gcggtgatct tttcgtcaat ttcggtgcgc atgggctgtg cgcagtggtg
      661 gtcgatctcg ccgtccagaa ctgcaaccag cgtcctgtcc tgcaatgtaa tttctactgc
      721 catcgggaaa ctccctgcct ttcctacaga caatgccgtg caaatccgct gggcgaactt
      781 tgtcggtgct gtctgatgtt tctatggcta gtatatcaca ggtgcagtgc cggaatctgt
      841 cgcaatgtgc ggtcagctgc ggctgaatgc acgcattttt tccatggcac gctgttccag
      901 ctggcgcaca cgctctttgg tgatgtgcag ttttccgccg atctccatca gcgtcatcct
      961 ttcacagttc tccaagccgt agcgcatggc gatcacttgc cgttctcggg gtgtcagctg
     1021 ttccaggcag gacgccagaa agagacggtc gcccacttct tcatctggtg ttggtatctc
     1081 cggatctgtc accatttcct gccgctcggt ctctccgtcc agcttcatgg tctcattcag
     1141 actgcgcagc gagaccatag gctcgacctt gcaaaggatc tccttgatct cagtctcatt
     1201 gatggtcttg ccgtttttct cttcggcagc cacccgtctg gacagcgcat catagctgac
     1261 cgctgtgccg ggttcccgat agacggaaag cttgtgcatt cggtcgtgaa gatgcaccgg
     1321 aatccgcacg atcgtgccgc cgcagataat ttcacgctgc actgcctggc gtatgtggcg
     1381 aaacggtttc cggtctccgg gcggtacagt ctcgccgcac ggagcagccc catctgtccg
     1441 gctgcccaga gatcttccag ttccagagaa agataggcat accgccggcg ctcccgccag
     1501 gcaatggtgt gtatcatgcc ctcgttttgt ttcagcagct ggagtttcgc ctgtgcgtcg
     1561 ccctgctgta cccggacaca taggctttcg ttgctctccg gatgatcggt cacggtctga
     1621 catctggttt gtgtttgctg cattttgatt ccccctcgaa atagacagtt cactttggct
     1681 gttctgtatt ccctattata cttgttcagt ttttcagaat ggatagaact agagaatttc
     1741 cgacaagaaa atgaaaaaga tcgtatgtga ttctcgggga aatgtatcaa cttgcacagt
     1801 ttttatagtg ccgcatttgt gtatttttcc tctggctttt tgtcgtaaaa tatgctataa
     1861 tattattata gtatatgaaa aaacgatagt gacttgagtt caggaagaga aaatacaggt
     1921 ttgctgcggc agtgcagctt gttgtaatgt gagccggcag ggtcggctct tgtgtggagg
     1981 aaactatgga aagcagtcag aataacacaa aaaccgaata tacaccaaac gaatatgtct
     2041 cttacggcac ttccggcgtt tgccagatcg tgtccgtgga acagcgttct tttgacggag
     2101 aacatgaaga atcctactat aagctgcgcc cggtcggaaa cgggaattct acttattatg
     2161 taccggtaga gcgtgccgca gaccgtctgc gtcccctgct caccaaggaa cagatctacg
     2221 ggctgatcga cgagatggca gacgtggatt tcagcgaaac agaatggtgc agcaacagcc
     2281 gggaacggcg tggcatcttt cagaatatcc tgcacagtga cgattacaag gaaatgatgc
     2341 agatgatgca caccctttat cagcagcagg aacggaaacg gcgcagcggt caccggctcg
     2401 ccgccgcaga tgaggctgcc atgcacgctg cggaaaaccg gatgtaccag gaatttggca
     2461 ttgttctgga catccagccg gaacaggtgc attcttttat tatgaaacgg atggatgccg
     2521 tttcttccac aaagtcataa taaaaaactc ctatagatga aaaaggttct tatcatgcaa
     2581 attgcacatg gtaagaacct ttttcgttat tctgttatcg gcagcgtcag tttcacgtgc
     2641 gtcagcgtca gaccttcccg ctggtaaaaa cggtgggcgt tggttcgttt ttggttgctg
     2701 gtcacttcga t
//
LOCUS       DAWENO010000232         2685 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_12215_length_2685_cov_8.224335, whole genome shotgun sequence.
ACCESSION   DAWENO010000232 DAWENO010000000
VERSION     DAWENO010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12215_length_2685_cov_8.224335"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          626 a          731 c          714 g          614 t
ORIGIN      
        1 catatgagat ctgccgcttg ccacggcaaa tgtaatatca cggcgatata attcctccag
       61 cagtgcaggc agctgtggcg gaagccgctt ttcattatcc agcagcgttc cgtccatatc
      121 tgttgcaatg agttgtatca tatccaattc tcccttcttc atgagacagg gaacgggagc
      181 ggagccgccc ggaaaaaccg ttcgcactgt ccgtatgata gtataccata aaaatgggaa
      241 aagtcaagga gcgggatgtg aattttttcg ggcaatgtcc cggaaacacg aaaatgtgcc
      301 gttttgcata cattagaatg caccgggaat gcctagattc ggtgcagaga ggagtatgtg
      361 cagaatgcag aaccaattgg aacgcatcca gcaaatagct gggatgcagc ggaattttct
      421 gcgggatttt gttccggcag atgcgccgga gagccggatg acggaacggg aatccttcca
      481 gcccacgacg ccttttccgg atcagacacc gccgggaatg gcatatgtgc cgtaccagca
      541 gtgggaagag ccgtatgatg cggacacagc ttttcccatc gggacaatgt tcccggcgtt
      601 ggattatcct taccgaggag gtggctcttt tgcatgagaa acagcggctg atggcgatga
      661 tccggaaata cgacttcgtc ctctacgagt tacagctgta cctggacacc cacccacgct
      721 gtccggaggc gctgcggatg tggaaaaact accaggcaat gcggcagaag gcagcgtccg
      781 cctatatccg ccagtacggt ccgatccagc cgctccagac agacggcaat gcgccctggg
      841 cgtggaatca gggaccgtgg ccgtgggaaa aggaggcgaa ctaaatgtgg gtctatgaaa
      901 agaaactcca gtatccggtg aagatcaaaa ccccgaatgc taagacggcg aagtatatta
      961 tttcacagct tggcggacca gacggcgagt tagctgcatc gctgcgctat atgaaccagc
     1021 ggtatacggc gcagccggat aaggtaaaag gaattttgac tgatgtggct accgaggagt
     1081 tagctcatgt cgaaatgatt tcagcgatcc tgtaccagct gacagacggt ttgacaccgg
     1141 agcagatcaa ggaggctggc tttgacgcct actttgtgga tcataccctg ggcgtttatc
     1201 ctcagagcgc agccggcgtg ccgtttactg cggcgtattt ccagtcaaag ggtgacccga
     1261 tcaccgatct gaccgaagat ctggcggcag agcagaaggc acgcagcacc tatgacaaca
     1321 tcctgcggat gtgcgaggat ccggatgtgc gtgatcccat caagttcctg cggcagcgtg
     1381 agatcgtgca tttccagcgg tttggcgagg cactccgcca ggtgcaggac gatctgaacg
     1441 caaagaactt ctactgctgc aacccgtcgt tcgatcagaa ttgcggaaaa actgcaaatc
     1501 ggagacgaat gcggtaaacg cagcagaaca tttcttacaa aaaacgtgtc actctgtcgg
     1561 tgcagaatga cacgtttttt tgctttatat tcggcacata tttcatgccg gttcttctta
     1621 tctcacttcg atgccatctc cgccagagtc cgcaccattt ccagatcgtc ctgggtcaca
     1681 cgcatattgg tgcagagttc ttttccctct gccgtagtca aactgatctc gatcacactg
     1741 ccctcgttga tcgcacggga cgctgccgag aagaacatcg gcactttcgg gtggttctgc
     1801 cggaactttg tcagcagtga tttcatctgc atcagcattt tcggattcat catatctaag
     1861 tcgctccttt tgtctgtatc atttcttgtg cagcggacac gcctcggaaa catgagggaa
     1921 cggcactgtc cggaactgca tccggctgcc gtccagcacc agcagctttc cggtgagcag
     1981 tgtgtcgctg cccagcagaa agcgaatcgc ctctgccgcc tgtatcgtgc cgatgatgcc
     2041 ggcagtaaca ccgatcaccg gaatcggcgc cggcgcatcc ccttccggaa tgtccccgaa
     2101 aatacagcgg taacacggac cttttcccgg cacataagtc atgacctgac cggaaaagcc
     2161 ggtaatgccg ccgtgtacaa agggttttcc ggcagccacg cacacatcgt ttatcataaa
     2221 cttatttttc agctgatctg ctgcatcgat cacaatatcg tattgttgca ccagtttttc
     2281 cagcgcaggt gcatccaaaa agcagggata cgctttcaca tccacgtggg aattcagccc
     2341 gtggagcgtc tgtgcggcgg aatccgcctt attctgtccc aggcgagact cctggtgcag
     2401 gatctgccgc tgtaagttgg acagctccac cctgtctccg tcggcaatgc caattgtccc
     2461 cacgccggct gccgccagat aataggctgc cggactgccc agaccgcctg cgccgattac
     2521 aaggacacgg ctgtttttca gacgttcctg tcccgtttcg ccgatctccg gcatcatgat
     2581 ctgccgggtg tagcgttcct gttcctgcgg tgtcagcata gcaattcctt tcccatcaaa
     2641 aatgtgcctg caaaccggtc agatccttga tgacctctcc ggcta
//
LOCUS       DAWENO010000233         2590 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_12693_length_2590_cov_11.979487, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000233 DAWENO010000000
VERSION     DAWENO010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12693_length_2590_cov_11.979487"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          509 a          799 c          662 g          620 t
ORIGIN      
        1 gtgccgatgc cccgatcatg tgggcatggc tggtgtaata ctcatagacg atgtcccggc
       61 tgtcaaatac cgggatcgca ccgtcccgaa gatattttat cacacccata tatcgtttgt
      121 caaatatatc tgccggcgga atgcaaaaaa catcccgtcc ctgttccatg gcgttctccg
      181 ccgtaatcag cgcaccgctc ctggcaggtg cctgtatgac aaaagttccc atgcttaacc
      241 cggacagcac ccggtttcgc aagggaaaat tcgatggagc cggcgatgtt cccggcagaa
      301 attccgacag gatcaagcca cgcctggcaa tttcatattt caaattggca tggggctggg
      361 gatatgcaac atccagtccg cagcccatca gcgcaatgct gggcttatcc gcctccagcg
      421 cacagcggtt cgccgtgatg tcgatgccca cggcatatcc cgtcaccgga acaaatccca
      481 tctgcaccag atcgtggcag atggtctttt ccacctggag actgtacggc gagggattgc
      541 gagttcccac gatcgtcagc agcaggtgtt cctgcaaaag ggagctgtcg ccctgataaa
      601 acagcagcac cggcggattc acaatggaga gcagctgcgg cggatacgcc tcgtcgccgt
      661 accagagaat ggacaggcgc agcttttcgc accgtgccag catttcttcc acttgtgcct
      721 cgccggtctg gcggatgcgc cgcagcgtat gttccggcag gtgcagtgcc gcatcggatt
      781 ccggcttttg cagcacctcc accgctttct ggggtgaacc ataccggtgg agcgctttcc
      841 agatccgtgg acttcctgcg ccgaacacca tcaccagcca cagcaggcag cgaatctctt
      901 ccatgtcact gccctccgtt tccggcggct gccgctttct gcacttccca caggataatg
      961 ccggcagcca tcgccgcatt gagggattcg atcgtcccgt gcatgggaat cgtcacccgg
     1021 cggtcacagg cggcagagac ttcttccggc aagccgttcc cctcgttgcc gatcaccacc
     1081 gccgtcccct tccggaacag acactgtccc accagttccg cctgtccgga gaccacagcg
     1141 gcgcaggact ccacaccggc atcccggcag gcagccagca ccggttcgat ctccggcaca
     1201 caacagagcg gcacacggaa cagagaaccc atggtcgcac ggaccacctt ggggctgtag
     1261 atgtcacagc ttgcgctgta gatcaccccg tccagcccca gagcgtctgc ggtacgcagg
     1321 atcatgccgg catttccggg gtcctgcagc tggtgcagca cggcatagct gccgccggta
     1381 tggatcagcg ccgaaagcgc cggcggctcc ggtatctgac agacggcgaa cacgccctgg
     1441 gtgtgttctg tctgggacag ttccgccgcc agactgtccg agacaagagc gcagcgtgtg
     1501 ccgtgcagca cgtccggcaa accctctgcc tgccagcgtg ccagaccgtc ctctgtccag
     1561 aacagctgtg tcagcgccgc accgtttttc acggcatccg acaccagccg aaagccctcc
     1621 agcacaaaca atccggtctt tgtccgttcc ttccgggaac gtgccagctt gcggtactgt
     1681 ttcaccgcag cattatcatg cgccgaaatt ttcagcagcg tttccatatc gttttccacc
     1741 agctttcgtt atggcagcac cgtgcagagc cgtcaggcgg actttgagcg gtactgccga
     1801 atattccgga ttttcctcat gatacaacaa aacacgcccc gtatcgtttc cagagcgtgt
     1861 ttcgcattcg atccttacag agcagcctta gcctgttcta cgattgcagt aaagcctgct
     1921 gcgtcgtgaa tggcgatctc agacagcatc ttgcggttca gtgtgatgcc agccttcttg
     1981 cagccgttca tgaactggga gtagttgatg ccgttcatct tgcaggcagc ggaaatacga
     2041 gtgatccaca gctgacggaa ctctctcttc ttctgctttc tgccgatgta agcatagttg
     2101 ccggacttca tgacagcctg ttttgccatc ttgaagtgct tgctcttgct gccccagtag
     2161 ccctttgcca gcttcagggt cttatttctt ctcttacgag tcatcattgc acctttaata
     2221 cgtgccatag ttatttacct ccgttatttc tcagattcca gtctaaagcc ctgtcgttcg
     2281 gcttagagat acggcatcag ctttttcagt gccggtgcgt ttgtgctgtc agcgatacct
     2341 acggtacgag cgattctctt ccgcttggtg tccttctgag tcagtctgtg tctcttgttg
     2401 gtcttctgat actttacttt tccggaagca gtcatcttaa agcgcttttt tgcaccggaa
     2461 tgtgtcttgg tcttgatctt tgccatggtg aattcctcct gtttacttcg atgccttcgg
     2521 tgaaatgaac atgaccatgc tgcggccttc cattttcgca ggcttatcaa caacgccgac
     2581 ttccttgcac
//
LOCUS       DAWENO010000234         2588 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_12705_length_2588_cov_8.261350, whole genome shotgun sequence.
ACCESSION   DAWENO010000234 DAWENO010000000
VERSION     DAWENO010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12705_length_2588_cov_8.261350"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1299..1308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          584 a          727 c          619 g          648 t
ORIGIN      
        1 acttcgatgg gataatcccc ggtaaagcac gccgtacaga atccgcactg cggcttcatt
       61 tccggcgttg ccagagactg cacgccttct acgctcagat atcccagcgt atctgctccg
      121 atatgagagc agatctccgg aatggtcatc tgacaagcaa tcagattttc cttgctgtcg
      181 atgtccacgc cgaaatgaca tgggttggta aaaggcggac aggaaattcg catatgtact
      241 tccgttgcac ccgcctcacg cagcagattg acgatccgct ggcaggtcgt gccacgcacg
      301 atgctgtcgt ctaccaggat cactcgcttt ccggcaacga cactctttac aacattcagc
      361 ttgatccgca cggaatcggt acgcatcttc tgattgggct gaataaacgt tctgcccaca
      421 tagcggtttt tgataaaacc gacttcataa gggatatgcg cagcttgaga cagacccagg
      481 gccgcatcca ggccgctgtc cggaacaccg atgacaacat ctgcatctac cggatgttcc
      541 ttccacagca gcttgccggc acggattctg gcatcgtgta cggtacagcc gttgatgaca
      601 gagtccgggc gtgcaaaata cacgtattca aacacacaca gagacggttt ctgaccgcag
      661 tgcgtccgga tggagcgaac ctcctgtccg tctgccacca cgatctcacc gggatccaac
      721 tcacgctcaa aggttgcctc tacgctgtcc agcgcacagg tctccgatgc aaaaacaacg
      781 ctgccatctt tccgccgtcc catgcagagc ggacggaatc cgttggggtc tctggcagcg
      841 atcagctttt gcggcgacat aaccaccagg gaataagcgc ctttcaggtc atacatggcg
      901 tattccacgg attcttcgat ggaagagcgt ttcaaccgct gttccgtcac cgcataggag
      961 atcacttcgg tatcgtttgt ggtatggaaa atcgcaccac gcagttcgta ttttctgcgc
     1021 agttcgtgtg cattgaccag gtttccgtta tgcgcaatgg acatgggtcc ttttacgtgg
     1081 cgaaccacga ttggctgcgc attggagcgg ctgggctttc cggttgtgga atagcgcacg
     1141 tgtccgatcg aaatattccc ttgtccgagc ttttcgatat gactgcggtt aaacacttcc
     1201 ggcacaagtc ccagatcctt atggtgtgaa aacacacccc ggtcattgac aacgattccg
     1261 cagctttcct gtcctctgtg ctgcagggca tagagcgcnn nnnnnnnngc aacatatgtc
     1321 atggatgcaa catctgttgt gtgaggcgca atgacgccaa acacaccgca ttcttcatgc
     1381 agtcctgtct tcaattacaa tcattccctt ccgttccagc aatacgaaca cagctggcag
     1441 tcgggtttac ccacggcacg gatcgtaccc tccagcgact gaaattccag cgacgtcagc
     1501 tgcagacggc ggcagatctc atcccggagc agctttccac gttctgtgtt agagttgctg
     1561 tactcgtcaa tatgctgcaa accagcaacg ccctcatttt catcaatgat ctgacgtgca
     1621 atcagttcca tctccgaagt gcttctggag aagttcagat atttacagcc gtacataatc
     1681 ggcggacagg cagaccgcat atggacttcc ttcgcaccgt tttcatagag aaactctacc
     1741 gtttcccgca tctgtgtgcc cctaacaatg ctgtcatcta cgaacaaaag ctttttgccc
     1801 cggatcagtt ccggaacagg aatcagcttc attttcgcta cccggtttcg catggactga
     1861 ttggctggca taaagcttct cggccatgtg ggtgtatatt tgataaaagg tctcgcaaac
     1921 ggaataccgc tgcggtttgc ataaccgatg gcgtgaggaa cgccggaatc cggaatgccg
     1981 cagacgtagt ccacgtccgg gagctttccg tttttcatgt cgttctccgc catgatctcg
     2041 ccgtttttgg tacgcatcac ttcgacattc acgccctcgt agcaggcggt gggatagccg
     2101 taatacgtcc agaggaaggt acagatcttc atttcgtctg ttccctcata gagtacctca
     2161 tagccatctt ttgtcagttt tgtgatctcc cctgccttta gctcatggca ggtctcgtac
     2221 cccagtttct gatatgcaaa ggattcgaac gagaaacaat atccgtcgga acgttttccg
     2281 atcaggatcg gcagcttgcc cgccttgtct cttgcgcaaa tgatcgaatc ttctgtcata
     2341 atgaccagag acatcgttcc ctcgatcttg ctctgcgcat agcgaatgcc ctcgacaaaa
     2401 gttggtttct gcgcaatcag cgcaccgatc agaccgccgg aattgatgtt gccgctgctc
     2461 attgtctcaa agtttgcaca gccgttttcc agcagttctt ccgaaagtgc ctttgcgttc
     2521 tggattatac cgatcgagca aactgcgtac aatccaagtt tcgaacgaac catgatcggc
     2581 tgaggatc
//
LOCUS       DAWENO010000235         2555 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_12881_length_2555_cov_8.112000, whole genome shotgun sequence.
ACCESSION   DAWENO010000235 DAWENO010000000
VERSION     DAWENO010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12881_length_2555_cov_8.112000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          549 a          762 c          720 g          524 t
ORIGIN      
        1 ccgccattga cccagcgagg atgccgggca tactgccgac gcagcagcag gatgtacagc
       61 gtgccgagcc atccacaaag caccagcgcc gcagtcatca ccggagccgt ccatccggca
      121 ctgccgctcc atatcgcaag cagcagcgga aagggataca aggcggcaga cagtgccgta
      181 atcagcagta ggtgcagcag aaagcgtgca tttttcatga tgccacctcc tcgctgtgca
      241 ggtattccgg caagggaatc ctcgcatcgg cacaactgcc gccgtcatgg gcgtgacccg
      301 tcaccagcac cagaccaccg cagcgctgtt tccactgcgc cgttgttctg tccgtgtacg
      361 gtgtgatgag cagcagtgtt tcctggatgg aagatctgcc gtgcatggag agcatctggg
      421 actgcggcac gatctcctgc aggcggagca gtgccagctg ctgcatgagt tgatggtaca
      481 ttccgctgcc gccgccatag ggcgacatga tcggctaacc gtcgtttggg cgctctcgca
      541 cctgccagcc gttccggaag aactcccaca ggcactgcgc acagacccgg atggtatgct
      601 ccaacagtgc ctcgtcctgc gtcataatgc cgctgtccgg gctgtgggat tccagcgcca
      661 gcaacaccgt gaccgtttgc tgtgcggtat gctcttcccg gcgcatgagc agcgttccgg
      721 cgtgcgccga ggctttccag tgcatccggt ggagcggatc gcccggcacg tattcccgga
      781 cacctgcgga aaggcagggg tccgtcagca ggctgtgccg gacagactgg tctccgaact
      841 gttggtgcag cagccgtggc agaatcagtc cagcctccgt catacgctgc ggcagaacca
      901 gtagcgttcc gccggtatcc tctgcctgga gcgacagccg caccacgccc agcgaatccg
      961 atgtgaccag caccacgtgt tccatctcgt atgcgccgcg cttttcacag gtgatgttcc
     1021 agacacggct gaccttggcg tgcccacgca cggaaaagaa actggtgaca aagcggtttt
     1081 ctccggtctg cgaggaattt gtctccggga agtccagcca gcaggacgtg gtcagttctg
     1141 ccttgagcca cggcacaggc atcccttttt cgttggaaac tgtctctaca aactgcaggg
     1201 agtcgccctc ggtgcattct gtctccgaaa agcggcagcg gtaggacagt cccttcaagc
     1261 cgttccggcg gtagagtccg atctcgccga acagcacgag cagtaccaac agaataacag
     1321 cgatcagttc cattgtgatt cctttggcac gggaacagtc tccagcagtt ccaacacgat
     1381 gcggcggcag gtctcgtgat tttcttccca cattgcccca cgtgctgcca tgcggtgcgc
     1441 catgcagtcc gctgcaatgt gcttgatgtc gtccggtgtg acatagtttc tgccacgtat
     1501 cgccgcaact gccttggaga cctccagcaa ggaaagcatt ccacgggtac tcagccccag
     1561 ctgtatctga tgatgcgtcc gggtggcgtg cgccaattct accaggtact cctgcaccgc
     1621 agcagagact ttcacctgcg ccagttcctt tcgggcgttc agcagctgtg ccgtggtgac
     1681 aaccggtttt ccctcttccg gaacagctgt ccgggacagc agtgccagtt cctgttcctt
     1741 atccggatag cccagagaaa ggcacagcag aaagcgatcc agctgtgcct caggcagcgg
     1801 aaacgtcccc tgcatctcaa ccggattctg cgtggcaatg acgaagaacg gcgactccag
     1861 cggataggtc acgccgtctg cggtcacctg gcgttcctcc atgcactcca acagggcaga
     1921 ctgggtacgt ggcgtagcac ggttgatctc atccgccaaa aggatctggg tgaacactgc
     1981 acctttccga aacacgaatt cgccggtttt cgggtcgggg atgctgacac ctaccaggtc
     2041 ggtgggcagc agatccggcg tgaactggat gcggtgaaag ctggcatcca aagcgtttgc
     2101 caaactccgg gcaagggtag tctttcctgt gccgggaatg tcattcagca gcacatgacc
     2161 gccgcacagc agcgcagcca gcaccttatc aataacatcc tctttgccgt agatgtgcgt
     2221 ggatacggca tcccggagca gtgtggtaat ggttttgatc tctgacataa acaaatgtcc
     2281 tttctggaaa tcagcagctt gtctcatctg ctgtcaatag cactattata acatatttct
     2341 gccggaaaca caagcaaaaa agcctgtctc cggagacgga aacaggcttt gaaatgcgtg
     2401 ttggaattaa aggaaaatat acttgaggat aaacagcagc gcaaccacgt atagcagcgg
     2461 tttcaccttc tttgccttgc cgcagacaac gtgcaggaca acataggaga tgatgcccat
     2521 ggaaatgccc tcggagatgc tgtagaacag cggca
//
LOCUS       DAWENO010000236         2547 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_12925_length_2547_cov_8.464687, whole genome shotgun sequence.
ACCESSION   DAWENO010000236 DAWENO010000000
VERSION     DAWENO010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12925_length_2547_cov_8.464687"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          537 a          718 c          674 g          618 t
ORIGIN      
        1 cggacggcct cgatgcagta gcctgcattc ggcaaaacga aaaatgccag ccccagcgga
       61 aacagtgcgc tgccgtgaaa cgcaccggct ttcagcgagt acagccagtc actgcgcagg
      121 acgatcaatg cgccgaactg caccaaaaga gtgcctgcca gcgtcagccg ggacagtgcc
      181 tttttagagc gcagcttttc caggagcagt cctgcaccat aggtcagtgc cgccagcaca
      241 agttctgtca gcgcagccat ccagccgccg cagaacagga aaaggatgct gattcccagc
      301 atgacttccc gtttccagcg gcgtggtgtc agataataga ttcccatggt aatgggcaga
      361 aagaaaaaca aaaaggtgaa agatgcaaat tgcatagatg ttctcctgtg cagtgaatgg
      421 gtcgaaactg atgtggtgca ggatgcttat gccagcgttt ccagcagcgc ttgcagttct
      481 gtgcgggtca tcatggtgtt cagaacttcc agcagcgacg ccgccgagag cagatccggc
      541 agccccagct ttttttgcag tgctttccgc cgtgccctgc tgtctgcgcc gccggagagt
      601 cccagctcat acagatccag ctttgtgatg gaatctgcct ttgccgggcg ctcgtctgcc
      661 agaatgcctg cctttgtgaa agcctccagg agccgtttct tatcgatgcc ctctacgccc
      721 agcttgccct ctgcggacgg cttatccttg cggcgttctt tgccgtaaat gtcggggatg
      781 tacacatggt acagctttcc ctcgtggatc gcacccttga gataatggcg gatcttaaag
      841 ccggcagtat ccgaatcggt caagatgatt acccctgttg ttttggcgta atagcggatc
      901 agagacatct tttctgcatc gtggaagatg cgaaaaccgt ttgtgacaag gatcaccgca
      961 tccagaatgg aggaaagctt gatcttgtcg tactttccct caacgatgat agcctgtgca
     1021 atatgaatca taggaacctc ctgcgatagc gagcattttt acgatggcag cttccagcat
     1081 gacccgttct tccccggagc tggaattcag atccagttcc agttcacgca gcacccggat
     1141 acacgcccga agacgctccg gcggcagcct gcggcagtca cggaacgcat tctgcaccat
     1201 aaagtcaaag gtatagccga agtcggcttt catatctgaa acctgtttcc ctgcccgaag
     1261 tgctgctgct gcacggtaca gatccaggaa tgcagacgca atggcgtgaa cgataaacgt
     1321 ccggttggtg cgcatggcat acagcttatc cagctcctcc atagcggctt tcgggcgctg
     1381 ggaaactacg gcttttgcca ggttgaatgt ggtcgtttcc agctgcggtg aggtcatttc
     1441 ccgcaccatt gctgtggtga tcgtaccgcc tttgtccgca taagcgcaga gtttttccag
     1501 ttcgctgcgc agaatgagag tgttgcccat gcagagacgc actagttctt cggcgctgtc
     1561 cctgggcatc acgcatccac ggcgttctgc cagctcggac agttcctttg ccagcacctg
     1621 cggtgtgcgc tggactgcct cacagacgat tccgcatttg gcaatgcagt cgatcagctt
     1681 tttatttttc ccctgaggcg tttttttgcc gtttttcggg tcgaacccgg tcacatccaa
     1741 taccagcacc gttgcgccgc cgactgtggg cagaaactcc agcagttgtt tcagctgatc
     1801 ttcccgcaga ctgttggcgt ccagatcatg cacctggatg cagttatggg cagaaaacat
     1861 ggagaactgg cgtgctgcat cttccagcgg tttcagttcc agcttttgcc cgtcgaaccg
     1921 ggtcagcgcc atgtctgcgt tgccgccggt cccctttttc agcaggctgg cggtcagctg
     1981 tttcacctgc accaggtcag cgccgtagat gtagtataaa ttttgcagtt cgccctttcg
     2041 aatctgggca gaaagctgtt tggggtcaca gatcggcata agggttctcc tttcatattc
     2101 gatggcgaac agatcacgaa agctgttcaa tatcccacgt gccatccgga tacacccgta
     2161 tctgaaccgc atcgtctttt tcataggttt cttccggcat ttgctccggg tctccgttcc
     2221 actgggtaca gatcagagcg tctacctctt cttccggaag ataggaaacg gctgtcccga
     2281 cacaaaaaca gaggttgccg aacttcacca gcagtgtgcc gtccgattcc aggatgctgt
     2341 actgggtgtc ctccacgaca aacttgtccg tttgcagcgg ttttgttccc ataaagtctg
     2401 catccggcgg aatccagcag ttggcaggaa ctgccgtttc tgcggcggtc atttccgaca
     2461 acgtctgcgg ataagccaca cgcagctggt cggctcgctt tgtcagacac agcgtatcca
     2521 aacggcgaat accttgttct gcgcagt
//
LOCUS       DAWENO010000237         2474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_13352_length_2474_cov_6.305912, whole genome shotgun sequence.
ACCESSION   DAWENO010000237 DAWENO010000000
VERSION     DAWENO010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13352_length_2474_cov_6.305912"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          600 a          681 c          558 g          635 t
ORIGIN      
        1 gtaaacaaca aaaaacgggg acacctcata aagagatgtc cccgcatttt atcgcgaatt
       61 gattttgtaa gaaaacttac ttcagttcga tagtagcgcc tgcttcttcc agcttagcct
      121 tcagagcctc agcgtcagcc ttcgggcaag cttccttcag agtagccgga gcggactcaa
      181 ccagagtctt agcttccttc agacccagac ccagagcttc cttagcagcc ttgataacag
      241 ccatcttagc gtcaccgaag gatgccagaa caacgtcgaa ctcagtcttt tcagcctcag
      301 ctgcgccagc agcgccgcct gcagccggag cagctgcaac agcagcagtt acaccgaatt
      361 cttcctgaat tgcgtcaacc agctcagcca gttccagaac agacagttcc ttgatatctt
      421 caacgaactt caaaaccttt tcagatgcca tgataataat ctcctttaac aataaattaa
      481 atgatttgca gagcgaaaat ctctgcgata cttatgcagc agtatcttcg gacttgctgt
      541 ccacaactgc ctgcagtgga gctgccagcg tctggatcgg accctgcaga gagccaacca
      601 gctgtgccag cagagtttcc ttcgacggaa tcttggacag cttttctacg ccggcagcgt
      661 cataagcaac gccttccacg tagccagcct tcagattgaa gaagtcttcg tgatcttctg
      721 cgaacttacc cagtacacga gccggagcag tctgatcgtc cttggacagt gcaacagcgg
      781 ttgtaccgtg cagaacttcg tcaaatgcgt cgatgcccat gttcttgaat gcaaaacgca
      841 gcatggagtt cttttcaact acatagtgaa cgccggcttc acgcagctcc ttacgcagct
      901 tggtgtcgtc ttcaacgctg atgcccttgt agtcaaccag aacgaaggat acgctgtcct
      961 gaagcatttc agtcagagca gcaaccttag ccttcttgcc ttccaaaatc ttttcacttg
     1021 ccatgagatt tcacctccga tagtttatgt atcgtatctg aaatcgcaat aaaaaagcac
     1081 gattcgacag ggaaccgtgc gctgtatttc acagatcatt gcacattcct cggttggact
     1141 tacggcgaaa cgccaccaac tgtctttaga atacgatatt gtgtttgaca actattacag
     1201 tatagcacac ttcaaagcgc ttgtcaagcc tttttttcaa aaaaatcaaa attttttccg
     1261 gatgcaaaaa tccctgccgc cgaaaccggc agcagggaag taactttgca gatttttctc
     1321 agcggaaaaa ctcagacacc atacttgttg gtagaaactc ttacgccaac gcccatggta
     1381 gaagtaacga cgcaggactt caggtaagta cccttagctg ctgccggctt tgcctttacg
     1441 atagcttcca tcagagtttc gaagttctgg gtcagcttgt ctgcgccgaa ggatgcctta
     1501 ccgatcgggc agtggatgat gttggtcttg tcgagacgat actcgatctt accagcctta
     1561 gcctctgtaa cagcctttgc tacgtccgga gttacagtac cagccttcgg gttcggcatc
     1621 agtccacggg gaccaagcac cttaccgaga cgaccaacca gacccatcat atccggggat
     1681 gcgataacta cgtcgaaatc catccagttt tccttggtga tcttgtcaac caggtcctga
     1741 ccgccgacat agtctgcgcc tgcagcctga gctgcctcat acttgtcttc cttgcagaat
     1801 acgcagacac gtaccttctt accagtaccg ttcggcagaa caactgcacc acgaacctgc
     1861 tggtcagcgt gacgggagtc aacgcccaga cggatgtgtg cttcgatggt ctcatcgaac
     1921 tttgcctttg cgtttgtgca aaccagagac agtgcctcgt cagatgcgta ctgctttgcg
     1981 ctgtctacag ccttgcggct gtcgttatat ttctttccgt gtttcatgat attttaatcc
     2041 tcctagtgta gtggtagtac cggattgctc caacgcagat ccggttagcg gaaatttcct
     2101 cccaccgacg ttcggctccc gttagtcaac gacaacgata cccatcgaac gggcagtacc
     2161 agcgatcatg ctcatagcag cttccagaga gccagcgttc agatccggca tcttctgttc
     2221 tgcgatcttc tgtacctgtt ccttggtgat gtttgcgacc ttgttcttgt tcggctcacc
     2281 ggaagcagtg tcgattccgc aagccttctt gatcagaaca gcagccggcg gagtcttggt
     2341 gataaaggtg aacgagcggt ctgcgtatac agtgatgaca accgggataa tcataccgat
     2401 gtcattcttg gtgcgctcgt tgaattcctt tgtgaacgcc ataatattta caccgtgctg
     2461 acccagcgcc gggc
//
LOCUS       DAWENO010000238         2426 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_13637_length_2426_cov_8.843104, whole genome shotgun sequence.
ACCESSION   DAWENO010000238 DAWENO010000000
VERSION     DAWENO010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13637_length_2426_cov_8.843104"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          621 a          628 c          610 g          567 t
ORIGIN      
        1 catgacggaa aaagaattat atcagaagtg gcttgaatgt gcaacagatg acgctgatct
       61 gcaaacagaa ctgaaggcaa ttgccggaga tgatgcagcg atcagtgacc gcttttaccg
      121 tgatctggaa ttcggtaccg gcggtctccg tggtgtcatt ggtgctggca cttaccgcat
      181 gaacatctat acggttcgcc gtgcaacaca gggtctggca aactatgtaa actctgccta
      241 cacaaacccc agcgttgcca tttcctatga cagccgcatc aagtccgatg tatttgcaaa
      301 ggctgctgcg gaagtgctgg ctgcaaacgg catcaaggta cacatctata cagagctgat
      361 gcctactccg atgctttcct atgcagttcg tgcgcagaag tgcagcgcag gtattatggt
      421 aaccgccagc cacaacccgg caaagtacaa cggttataag gtatacggca gcgatggctg
      481 tcagatgacc attgacgccg caaatgcagt tctggctgag atcaacgcac tggatatttt
      541 ccaggatgtc aagcataccg atttcgacgc agccatggct gacggcatga tttcctacac
      601 accccagtct gtgattgacg cttattacca ggatgtgctg aaacagagcc tgaatccgga
      661 tctgtgcgca aagagcggtc tgaaaattgt ttacacaccg ctgaacggca ctggcaacaa
      721 gccggttcgc cacattctgt ctgcaatcgg catcaaggat gtaactgttg ttccggaaca
      781 ggaaaatccg gacggcaact tcactacctg cccgtatccg aatccggaga tccgtgaggc
      841 tctggaatgc ggtctgaaac tgtgtcgtaa agtcaagccg gatctgctgc ttgcaactga
      901 cccggatgct gaccgtgttg gtattgcagt accggacggc gatgattacg ctctgttctc
      961 cggtaacgaa gtcggtgcaa tgctgctgga atacatctgt cagcagagaa ttgaaaaggg
     1021 tactatgccg aagaatccga ttgctgtcaa gaccatcgtt accactgaca tcgtcaatgc
     1081 tattgccaag gaatacggcg tagaagttat cgacgttctg accggattta agtttatcgg
     1141 cgagcagatc ggtttcctgg aagaaaaggg cgaggaagac cgctatatct tcggatttga
     1201 agaaagctac ggctatctgg caggctctta tgttcgggat aaggacgcag ttgttgcatc
     1261 catgctgatt tgtgaaatgg ctgcttacta ccgcaccaag ggcatctcca tgatcgaggc
     1321 tcgcaagcga atgtatgaga agtacggcgt ttacgttcac agccagcaca gctttacctt
     1381 tgagggcgaa agcggcatga tcgagatgca gaacatcatg acaaagctgc gcaacgagca
     1441 tccgaccgag atcaacggtt tgaaggttgt aaagtttgac gactaccaga ccagcgtctc
     1501 caaggatctg accaccggcg aaaagacaga gatcaccctg ccgaagtcca acgttctggc
     1561 attcacactg gaacagggcg caaaggttgt tatccgtccg tctggtactg agccgaagat
     1621 caaggcttat tacaccgtaa ctgcggatac caacgaagag ggttctgtaa aggaaaaggc
     1681 actggatact gcattcagcg caatgattct gaactaattt tcacttgttg aaataaagca
     1741 aatgctctgt ccatgtatat gggcagagca tttttgttgt ccgataacag aaaagcggag
     1801 ggtttctgct gaatcctccg cttttcgttg ttttgcttat aaagtgtggc agacttctgc
     1861 aaacggcacc agctttttca aagctgtaca cgcctgttcc gccgctgcac gagtcgggaa
     1921 caacccaaac actgccgcac cactgccggt catcacgctg caaagcgcat cgtgttccag
     1981 catactcttc cgaattgcag caacttcgtc caggtcaact gccagttcga acaggttgcc
     2041 gcaaagcggt gcaatttctt ccgcagttgc cccatttcgc aacgcctgcc gcagttcctg
     2101 cacaggcgga tgctgtggat tttgcaaagc gtcaatcgca ccatatgcct gtgcggtgga
     2161 aacgcctgct gtccccttgg caatcaccat gcagtccgta gaaaacgccg gcagtttttc
     2221 caatttctcg ccgattccgg cagcatatgc cgttccgccg tacaggaaaa acggaacatc
     2281 tgctcccaga gataccgcaa tttcagccaa tttatccatg ctcaaagcat tcccggttag
     2341 ttcctgcaat gccagcaaaa cagccgcacc gtctgtgctg ccgccgccca tgccagcctc
     2401 catgggaatc cgcttttcaa tgtgta
//
LOCUS       DAWENO010000239         2283 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_14538_length_2283_cov_8.757181, whole genome shotgun sequence.
ACCESSION   DAWENO010000239 DAWENO010000000
VERSION     DAWENO010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14538_length_2283_cov_8.757181"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          468 a          662 c          586 g          567 t
ORIGIN      
        1 tttcagattc cggttgttga cgcccaggat ctgcggctgg aggaatgcca cgctttccag
       61 ttcctcccgg tcgtgtacct ccaccagaac ttccagcccc agcgaatacg ccaggcggta
      121 cagttccgcc agacgctccg gcggcaggac tgcggcgatc agcagggcgg catctgcgcc
      181 aatggcagct gcctcatata cctggtactc gtcaaaaata aagtctttcc gcagaatcgg
      241 cagggaaact gctttccgga ttgccgccag gtattccgac gagccttgga aataatgctc
      301 ttccgtgaga caggagatgg cgtttgcgcc ggctgcctca taggtcttgg caatttccac
      361 cgggtggaaa tccgcacaaa tcagtccctt ggacggagac gcttttttta cctcgcagat
      421 gcaggaaatg gtatcctttt tcagagccgc tttcagcgaa accggtttgc ggtcagccag
      481 agctttttcc gcccggactt tcattgcatc cagaccgcag gcagcctttt cccgttccag
      541 ctgctgcgcc cggtatttca taatatcatc taagatcatt ccagcacctc acgcattccg
      601 gttggtgaat tccaccagtt catgcagctt ttccagtgcc ttgccgctgt cgatctgctg
      661 ttctgccagc cggataccat ctgcaatggt tgcagccttt ccggaaacat acagcgcaca
      721 gccggcgttc atcagaacaa tgttccgctt tgcgccacga atggaaccat ccagaatgcc
      781 acgggtgatc ttcgcatttt cgtctgctat accgccgacg atttcttcct tggatgcccg
      841 tgggaaaccg aaatcctccg gtgcgatctc gtattccgtg atcttgccgt cacgtacttc
      901 tgcaacggat gttttatcgg aaatggagac ctcatccagg cggtcattgc cgtagacgat
      961 catagcactc ttgataccca gtcccaggag aacacgtgcc attaccggca gcatcgcctt
     1021 ttcatataca cccagcagga tgtagttggt ggatgccgga ttggtcagcg gtccgaggac
     1081 gttgaacacg gtacggatgc ccagttcctt tcgagccggc gcaacatatt tcatagcgcc
     1141 gtgatagctc tgtgcgaaca ggaaggaaat gccgatctcc cggacggatg cctttgcctg
     1201 ctccggtgtg gtctgaatct tcacacccag gctttccagc acgtctgccg caccactctt
     1261 gctggaaacg ctgcggttgc cgtgctttgc caccggcatt ccggcagcgg aaattacgaa
     1321 agaggaagtg gtggagatgt taaacgagtt tgccaggtcg ccgcctgtgc cgacgatctc
     1381 aatggactct tcacagccgg tgacgtgctt tgccttgttc cgcattccct tggcgaaacc
     1441 ggtaatttcg tcgatggtct cgcccttcat gcgcagtgcc accagcagtc cggcgatctg
     1501 tgcgtttgtc gcactgccgc tcatgataat atccatggca tcctgtgcct cttccaatgt
     1561 caagtcacag ccgtctgcca cttttgcaat atactttttc agttctgttt tcggacgatc
     1621 ttccacaatg ggcggcagtt ccacagacac accgggaatc agattcaaga agttcgccag
     1681 catccgcttg ccgtcaccgg tgagcagcga ctccggatgg aactgcacgc cgtaggtctg
     1741 tgtctcacgg tgctggagcg ccatgatctg accgttttca tcccgtgctg ttactttcag
     1801 gcactccgga aaatccgatt cctgggcaat cagcgaatga tacctgccgc aggaaaccat
     1861 tttcggcagt ccggcgaaaa tggggcttgt ggtatccagt accatctggc ttgccttgcc
     1921 gtgcatcaga tgcgctgccc ggacgattgt gccgccgaat gcctcgcaaa tggactgatg
     1981 tcccaggcag acacccagca taggaatctt gccgttgaag tgacgaatcg cctccatggt
     2041 gatgccggca tccttcggat agccgggacc cggcgaaagg atcagcgcct gcgggtgcat
     2101 ctcctcaatc tgttctattg ttatttcgtc attgcgtacc acctggatgt catgatacag
     2161 ttcgccgata tactgataga gattgtaagt gaatgaatca taattgtcga tcaataaaat
     2221 cattgttctg cctccttgat ggcgttgatg tcgccccgta cggcgtcaaa ctccagccgg
     2281 ggc
//
LOCUS       DAWENO010000240         2243 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_14821_length_2243_cov_9.730347, whole genome shotgun sequence.
ACCESSION   DAWENO010000240 DAWENO010000000
VERSION     DAWENO010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14821_length_2243_cov_9.730347"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1843..1852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          580 a          527 c          645 g          481 t
ORIGIN      
        1 atatcccggc gctgatggaa cagtatttca gccagctcta tgacatatgc cgctggggtc
       61 acttcgatgt gctgggtcac cttacctatc cgctgcgcta tatcgagggc gaggcaggga
      121 taccgatacc gctggaaccg tatgcggaac agatccgctg ttgtttccgg gcgctggcgg
      181 agaacggaaa gggcattgag ctgaacacct ccggctatcg ccagaagtac ggcaagccct
      241 ttcccacgct ggaatacttg aaactctatc gggagatggg cggcgaggtg ctgacctttg
      301 gctcggatgc acactgtgcg gaagatctga ccaaagggat cgccgagggc gtggcgctgg
      361 cgaaagcagc cggctttaca cgagcctgtt attttgtcaa acgaaagccg gtttttataa
      421 cactttaacc gggttcaaat attattttca gaaaggctgg gtttctcatg aaagaacaat
      481 tactggaatt attgcagcag aacgcccgac tcaccaatgc ggagctggcg gttatgctgg
      541 acgtgacaga ggcagaagtc gccgacgcca tccgagcgct ggaaaacgag ggtgtcatca
      601 agggttacag tgcgatcgtc aatgacgagc ttgccaacaa agacctggtc accgcagtga
      661 tcgagctgcg tgtaacgccc cagcgtgact gcggctatga agaaattgca cataccatca
      721 tgcagtatga cgaagtggag agtattgcgc tgatggcagg tgcatatgat ctgtcggtta
      781 ctgtaaaagg tacaaatgtg aaggatgtct ccatgtttgt atcgcagcgt ctggcgaccc
      841 ttgccggcgt gctttccacg gcaacctatt tcgtgctgaa gaagtacaag gaaaagggta
      901 tcattatcga aggtgaagag aaagacgaac ggggctatta tttcccgtaa ggtcggaaac
      961 aggaaagggg agagaactcg tgttgaacta tgacacgctt ctttcagatc aggtaaagaa
     1021 aatgaaaccg tccggcatcc ggaaattttt cgatttggct gccatgatgg atggcgtaat
     1081 cagccttggc gtgggcgaac cggactttca gacaccgtgg atggtgcgga aagccgcagt
     1141 ggacagcctg gaacgcaagc gcatggtata tacagcaaat tccggcatgg cagatttgcg
     1201 gcaggagatc tgcaattatc tgaaacgcaa atacgatttg acataccatc cggaaaatga
     1261 aacactggtg acggtcggcg gttctgaggc aattgacctc gccattcgtg ctattgtgaa
     1321 tccgggagac gaagtgctga tcgtggaacc gtcattcgtc tgctataagc ccattgtgga
     1381 actgatgcac ggcgttgcga ttccgatttc gacacagctg aaaaacaagt tcaagctgac
     1441 ggcggaggaa ctgcgttccc acatcacaga acgtaccaag ctgctcattc tgccgtatcc
     1501 gaacaatccc accggtggca ttatgacacg ggaagatctg gaggcaattg cagaggtact
     1561 gcgggacaca aatgtgctgg tactctcgga tgagatctat tccgaactga cctatggcag
     1621 aaagcacgtc tccattgcgt cgatcgacgg aatgtgggaa cggacgatct acgtcagcgg
     1681 tttctcgaaa gcgtttgcca tgaccggctg gcggctgggc tatgtctgtg cgccggaacc
     1741 gattgcaaag cagatgctga aaatccacca gtatgcaatc atgagtgcgc cgacactgag
     1801 ccagtatgcc gcaatcgtgg cactccggga gtgcgacaaa gannnnnnnn nnaagaagta
     1861 cagaagatgg tggacgaata caaccgccgc cgccggatgc tggtagacgg tttcaaccga
     1921 atcggactga cctgctttaa tcccgaaggc gcattctata tgttcccatg tattcgcagc
     1981 atcggcatga gcagtgagga attctgtgaa acactgctcc aggaagaaaa ggtggctgtt
     2041 gtaccgggca atgctttcgg ggagagcggc gaaggatttg tgcgtgtatc atatgcgtac
     2101 tcgctgaaac atctccagga ggcactggtt cgcatcgagc gctttgtgaa ccgtcacaga
     2161 ggagaatagg catgagaaaa gtcacgagaa ctgcaccggc gaaaatcaac ctgggcttag
     2221 atgtcacagg aactcgcccg gac
//
LOCUS       DAWENO010000241         2226 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_14939_length_2226_cov_11.856287, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000241 DAWENO010000000
VERSION     DAWENO010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14939_length_2226_cov_11.856287"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          520 a          558 c          613 g          535 t
ORIGIN      
        1 acgacacccc ggacacggga gttcgtcaac ttcttcctgc gggaaaacca gtagggatcc
       61 tgtgtgcatt acgccacagc cggaacgata cttgcccggc atctgggcat tcctgcccgg
      121 tactgcgagg gctatcttgt gggcgaggac attctggaca gcgcatcggt ggattctgac
      181 ggttacaccg tggaactaaa agaccggcag gcgcacgcct ggtgcgagtt ttatgtggac
      241 ggttacggct gggttccctt tgagatgacg ccgggctatt atgacagcac caaggtcggt
      301 gatgtggagg ttgctgactc gacagaaccg cccacaaata caacgcagga aaccacgatg
      361 gatgcagcag catctaatgt cgtctccacg gaaatgacaa gcgcatctgt gacttctgcc
      421 gtttcgtcgg gagaaagcac tgcgtcagaa acggagccga ttcccggcgg cacagagcca
      481 gatccgaatg gcggcggtct cttcgggaat accggcagtg cagcgaatct ggttcggctc
      541 tttatacaca tcttcctctg gctgctgctt gcggcagttc tgaccgcagc ggtgatcctt
      601 ttgcggcggt ggcacataca gcggcgcaga cgcatctttc tggataccct gcacccgagg
      661 caggcagtct ttgccatgta ccgctatctg ttccggctgc tgcgttggac gggacttcgt
      721 ttcgaaaatg agccgctttt ggaattctgc gaaacagctg tccggcacct ccggcagaag
      781 gatttgcctg cggatgcacc ggagatcatc attcccatga cgcttgcgct ggacttcggg
      841 aaacagttcc cggaaaagga tgtccagcga aaagctgcgg catccgtaga agaactggct
      901 aacgccattt gtgcatcctg caaaccgttt cggcggttct gcatgaaata tattctgcat
      961 ctatgctgac gattgaaagg agttttctta tgagtactaa aacaccaaaa accaaattcg
     1021 gaaaacaaat gctttacatc ctgctgcctt ttgtgctgcc gctctgtctg attattctgg
     1081 gcattgttct gctggcaaac gcaaaggtag tcaacggcat cttccttgcc ctgggaattc
     1141 tgattgccct ggtggggctg gtggaagttg tgatctacgc atcccggaaa aagtttcagc
     1201 cggagtcccg gtatctggtc acgggaatca ttctcatggt aatcggcgca ctgctcatca
     1261 tcctgccctt taccatcaac aatctgattc ccattattct gggacttagc gtattgggca
     1321 gtggtatttc cggcatcacc aacaccctgg ctttccgccg agaggatacc aacattctgg
     1381 catccatgtt gtttgccgtt accaactgcc tgctgggtgt atttattctg atctacgttc
     1441 tgttcatcag cgaccaggca ggctggaatg tgatcggtat tcttatgatt atcagcggtg
     1501 tgctgcgtct gatcaacgag atcattgcca gagtagcgcc aaagcattcc ggtgctgtga
     1561 ttgagacgac atacacagaa actaccacag aggccacaga accgccgaaa gaaaactaag
     1621 ggagtgtcac acgaatgtta ttagcgaatt atcacaccca cacggctcgc tgccggcacg
     1681 cctggggtga ggacaaggaa tatgtggaaa atgccattca agagggcatg aaggtgctgg
     1741 gcttttccga tcactgcccc tggattttcc cgaaggatta tgtttctcac acccggatgg
     1801 aaccacagga actggacggc tacttttatg cgctggaggc actgcggcag gagtatgcca
     1861 gggatattac catttacatt ggctttgaat cggagtatat tccggaactg atggaggaac
     1921 agaatcagct gctggacggc tatccggtgg attatatgat tctgggagaa cattttactg
     1981 ccagcgaagt gaccggaccg tataccgggt tccctaccga gtcggaacag gatctggaaa
     2041 agtatgtgga tctctgcatc gccggaatgg aaaccggaaa gtatgtctat gtggcacatc
     2101 cggatctgat tcattatact ggtccggatg agatctacga agagcacatg gaacggctct
     2161 gccggtatct caagggcaag gatattcctg tggagatcaa tctgctgggc gtggtggaaa
     2221 agaggc
//
LOCUS       DAWENO010000242         2206 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15091_length_2206_cov_8.901906, whole genome shotgun sequence.
ACCESSION   DAWENO010000242 DAWENO010000000
VERSION     DAWENO010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15091_length_2206_cov_8.901906"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    246..260
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          452 a          606 c          582 g          551 t
ORIGIN      
        1 gaggaaagtt ccgtgccgtt cagtaccagg tcgaatgcct ttgcggtgac acgtgccgga
       61 tcgctgtcca gatagggcag gcactcgtcc atcggcatgg tgaacgggtg gtgcatcgca
      121 tcccagtgct gggtgtcctc gttccactca aagaacggga actgggtgat ccacaggaag
      181 ttgaacttgc cctccgggat cagatccagt ttctttgcca cttccagacg cagtgcgccc
      241 agtacnnnnn nnnnnnnnnn tcatccagct tgccctccgg caggaacttc gcaaaggaac
      301 aggtaggctt ttcgtcgttc cagcgaatga atgccagtcc ctttgcgccg atgccctttg
      361 ccttttctgt cagcttgtcg atttccttgc gggtcagcac agatgcggca ttctttgcca
      421 caatggcacg aacgctgccg ccggcattca gcgcagagcg gaataccata aagtcaacat
      481 ccttgactac atcggacaga tcctggagtt ccatgccaaa gcgagtgtcc ggcttgtccg
      541 aaccatagcg ttccattgcc tcctggtagg tcatgcgggg cagcggtgtg gtgatatcca
      601 tgttcagcac tttctggaac agatacttga cgaatccctc tgtgatctgg agaatatcct
      661 ccacatccac aaaggacatt tccaggtcga tctgggtgaa ttccggctga cggtcagcac
      721 ggagatcctc gtcacggaag cagcgtgcga tctggatata gcggtccatg ccggaaacca
      781 tcagcagctg cttgtagatc tgcggtgact ggggcagggc atagaatttg ccgttatgca
      841 cacgggacgg gatcagatag tctctcgcac cttccggtgt ggacttcatc atcatcggcg
      901 tttcgatctc gatgaagttg ttggcataga agtactctct ggcagccagt gcaatcttgt
      961 ggcgcataat cagattgtgc tgcagcggcg cacggcgcag atccagatag cggtatttca
     1021 gacgcagttc ctcgttggtc ttggtctcgt cggtgattgc gaaaggcggt gtctgtgcct
     1081 tggacagtac acgcagatcg tcaaccaaaa cctcaaaagc gccggttttc atttccttgt
     1141 tgacgctgga acgctctgcg actacgccct ttgccatcag cacatattcg ctgtggcagg
     1201 atgcagcctt ttcgaaaatg gcacggtctg tctggtcgtt gaatgccagc tgtacaatgc
     1261 cggtgcggtc acggagatca atgaaaatca gattgcccag gtttcgtacc ttctgtacaa
     1321 agccgccgac aacgacagtt tctcctgctt ttgtcacttc accgcagtaa caggtgcggc
     1381 gcaaatcctg catcaaatcc atgtttactg acactccttt tcaaaatcct ccacgatgtc
     1441 gtggaacata tactggacgg cgatatcgtc gaaattttct tcaaaggtct cgtccagagc
     1501 gatctctgtc tgctcgccgg tctccatatt tttcagtttt gccttctttt ctgccagctc
     1561 atttccgccc agcaccatta cgaaggacgc accgatcttg ttggcatact tcatctgtgc
     1621 tttcagacca cgctgcatca catcgtattc gacctgatag ccggaggtgc ggagctgctg
     1681 tcccagctgc attgtcagcg gaacggcgct ttcgtccatc ggggcaatgt acaggtcgca
     1741 ggacttcggc tcttcgaagt cgcagccctg gctctccatg gtgaggatca gtcgttccag
     1801 acccatggca aatcccattg ccggtgtggg ctttccgccc atctgttcga tcagtccgtc
     1861 ataccggccg ccgccgcaga cagtaccctg tgcgccgatg tcggtggtga caaactcgaa
     1921 aacggtcttg gtataataat ccagaccacg aacgatcttc gggttgatgg tgaagtcgat
     1981 attcagcgcc ttcagatatt cctggagctt ttcgaagtga gtccggcagt cgtcgcacag
     2041 gtagtccaga atgatcgggg catccttggc gattccggcg cagatcgggc ttttgcagtc
     2101 caggatacgc atggggttct tttccagacg ggtgagacag gtctcgcaca gctcgtcctt
     2161 tttcggttca aaatacgccc ggagcgcctt gtggtattcc gcacga
//
LOCUS       DAWENO010000243         2167 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15362_length_2167_cov_13.020833, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000243 DAWENO010000000
VERSION     DAWENO010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15362_length_2167_cov_13.020833"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          472 a          618 c          623 g          454 t
ORIGIN      
        1 aagggacagg gggatatccc tccggcactg cgtgccacct cccttttcaa gggaggcaaa
       61 agactcctct gccctgtcgg gcatctcgta ctgagcggtc ccttttgtca cctgcggtga
      121 catttcccca ccccgtgggg aatcacctct atgagaggag acgatttcgc agagcctcct
      181 ctttagagga ggtggcaagc cgtaggcttg acggaagagt ttcgggcgaa cgatgttcgc
      241 ccctacggtg ttgtttttcc cggatgacaa ggcaatactt ctgccataca aaaaacgcac
      301 tgtcgtcacg catttcccta ccgatacatc cgtgtgagtt ccaccaggtg ggagatgccg
      361 gggatgcttt cgccctgctg ggatgccgtg ggggacatga gcgcaccggt tgcggcgaac
      421 aggatctgcc gcatcgttcc cgcctggagc gccgggagaa aatggccgca cagcaggctg
      481 gcgctgcatc cgcagccgga accgccggcg tgggtgtcct gtgtctccgg gtcgtagagc
      541 attgcgccgc agtccttatg attccgggtg atgtcatagc cctgtttcat caccagatcg
      601 cagagcagtt cgctgcccac gatgcccaga tcgccggtga cgatggcgtc atactgttcc
      661 ggcgcagttt gggtggcttt gagatagcgc acgatggtat ccgctgccgc cggcgccatg
      721 gctgcgccca tatggttggc gtcggtcact cccagatcca ccatgatgcc gatgcaggca
      781 cccgtgacgc acaccccgtc cggaacgccg tccggctgct ggagaatcac agcgcccgat
      841 ccggtacagg tccactgcgc cgtaggcggc cgctgaccgc cgtaggagag cggaaaccga
      901 aactggcgct ctgccgtgga aaaatgggaa gaagtcactg ccgccgtata ttcgcccacg
      961 ccgcctgccg ccagaaacga cgctgtcagc agtccctccg ccatagtgga acacgccccg
     1021 tagatgccca gcaggggaat ctccagcgga cgcattccat aggtggagcc gatgcactgg
     1081 ttcatgagat cgccggcgca gatgcaggag aggtcgctgt cccggagtcc ggacttttcc
     1141 agcgccagct gcaccgtgcg gcgctgcatg acgctttccg cctgttccca ggtctcctgt
     1201 ccgaaatggg cgtcgggttc tacacagtcg aaacagctgc ccatgggacc gtcgccctct
     1261 ttctgaccca cggtgctggc gtagctgatg atgcggggcg gctggggata ttgaaaaacg
     1321 ccccgggcaa gattacgtac cattcgaatc acctcgatgg tagtatatcc gggacgttgg
     1381 gaattatgcg agtgagaatg caaaataaaa ttgcattgcc actcgtgtga tacattgccg
     1441 tttgaagtaa gggtaggcga aagcggcggg tcagcccgtc gcccagtgga aatggcgacg
     1501 gcagacttgc ccaaggatag caagccgccg cagacctttc gccccaattt aacattcaaa
     1561 aaaggacagt gtcacggcag acaagctgcc tataacccct ccgggggatt tttgttatat
     1621 gtaatctaat ggcagttaca ctgccatccg tgtaatacat tgccgtacag aaaaagggta
     1681 ggcgggagct tttctttttt tgcttctttt tttcttttca gtaaagaaaa aaagaagagt
     1741 aactcttggg agaatttttt gaaaaagatt gacgggcgaa caatgttcgc ccctacagat
     1801 ttgcgtggca aacgccataa taataacgtc aaccgcctgc acaagagaca gtcaaaaaag
     1861 cagcgttata ggtacaggaa agggcaaaca aaggcgtttg caagccccag gcggacagcg
     1921 agggaaacga gcgattccgc cggacgggtt gaacaagttt tccctagggc agtgtttccc
     1981 ggaacgacgg ggcgatgtgg cttgctatcc ttaggcaagc ctgccgtcgc catttccgct
     2041 gggcgacggg ctgggcatcg ccccctacag atttaccttt tcatgaaaga caaaacaact
     2101 cctctgccct gccgggcatc tcgtactgag cggtcccctt tgtcacctac ggtgacattt
     2161 ccccacc
//
LOCUS       DAWENO010000244         2159 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15420_length_2159_cov_9.245722, whole genome shotgun sequence.
ACCESSION   DAWENO010000244 DAWENO010000000
VERSION     DAWENO010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15420_length_2159_cov_9.245722"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..247
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1275..1284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          566 a          480 c          579 g          504 t
ORIGIN      
        1 gactgcacac agaggatgtg ttttttggag atagagcagg cgcttatacc aggaatcctg
       61 tcaccagcgc tgctgccgat gctgcaagtg ctaccacgat cagcggcagg ttgaagtaag
      121 ccagcacgac cgcaacgacg gttcccaccg ccgcagtcac catgctgccc gtggagtaaa
      181 agattgccgg aatggtcatg gcactcagca ccgcataggg aatgtaannn nnnnnnnnnn
      241 nnnnnnngca cacagaatga tggcgaatcc gctggagact gccgtaaaga catatttgaa
      301 gaggatgctg cacagcgccg ccagaatcac cacggtgagg acgcttttct ttttccgggc
      361 taccggtacg atgattgcca ggaacatacc gtacagcaca atgcccatgg cgtcaatgat
      421 aaacttcggc aggatctgtc cggcagcagc acccagcagt gtccccagta cccagcccag
      481 gaatgcaatg gcaatcagcc cgtacagata tgccgggcag atcattcccg gctgggaaga
      541 ggcaacggcg aagatttcgt cagtgatgcc gaacgccgtt gtcatgcgct ggggcaggtg
      601 aaacttttcg tccagtttct gggacaggga aaagcccata agggcatagc ggaagttgat
      661 ggtcagctgg gtaagcgcca tttccagata agaaccgccg gcagcgataa cacctacgcc
      721 ggcagcctgt ccggcggagg taacattggt gacagaaatt gccacggcgg cagggataga
      781 aagcccggca cgcaccgcca ggatgccgaa tccaaaggag acggagagat agcccagggc
      841 aatgggaatg ccctggagaa cgccttttcg aaatgctttg ttcaagatga tgatgtcctt
      901 tccggagcct gcggacaggc tcgtatcaaa tgtcacagcc ataacggcac agtaactttt
      961 attataccat aaaccgatgg gaaatgcaaa ttttttctgt cggttttctt tgtggggaaa
     1021 atatgcaata tatggagaat cgtcttgtgc tttcttgtga aatgtgatat aatggaaata
     1081 tacgaagaac gaggacattt tttgaaagta tgtttttgag gtgatgaaaa tgtggataac
     1141 actgcttgcg gcggtcatga ttatggcggc gtactttctc attttgtatg gcggtgtcgg
     1201 ctttgtccag gataagcggt tcttttcctc tgcgccaaag gaaaatctgg cggcaattcc
     1261 cgacacaaag gaacnnnnnn nnnnggcgct cacataattg gctggctgat tatcgcagct
     1321 gcactgctca cgtttctggg cgcagccgct cttgcgatag gggacggaat caaaaatgat
     1381 tttggttttc tgcaattctt tgtgcggttt cttgtgatgc tctatctgat ggaagtgtac
     1441 gacatcttct tttttgactg ggtactgctc tgtcactcga atttctttcc ccacttttat
     1501 ccggagctga aaggcatcgt cgggccgcat atgttcggat acaacaagaa aagccatatc
     1561 atccacttta tcgtgtacat tcctgcctgt gccttggttg catggatatg cacgcttttt
     1621 tgagaatcag acgttgcgag gagagaaaac atgaagaata cacttaatgt aaaagtactg
     1681 tatcactttt atcacagcgc agtaaaagca gagctgaaga gacgaggttt ttccaaggat
     1741 gtctccggga agatcaatga agaacacaag aagatcatcg ggagggcaaa agaaatcgga
     1801 aaatcccggc ttttgagttc ttacattatg gcaacctatt tcattgcaat gaaccggagt
     1861 accggaaagc cggctgagga aaattatgag atacttcgtg acggactgtg cgcatcgaag
     1921 ttattccaca aggcaatcgg ggatgtggag cattatttag acccgaagaa aatgccgggc
     1981 agactgcaat ggtctgcgga cagccataag cgccgctatg aaaacgactg ggtcgttgat
     2041 attctgccgg gaaacgggga atatgacctg ggatacgact atcatgaatg cggtgcatat
     2101 aaactgtgcc aggatgaggg ctgtccggaa ttgaccgcct atatgtgccg gatggacta
//
LOCUS       DAWENO010000245         2155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15455_length_2155_cov_7.279524, whole genome shotgun sequence.
ACCESSION   DAWENO010000245 DAWENO010000000
VERSION     DAWENO010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_2155_cov_7.279524"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          552 a          526 c          554 g          523 t
ORIGIN      
        1 aaaatagatc tgattacagg tattcttggc tccggaaaaa ccacattcat ccacgagtat
       61 gcgtcgcatc tgattcagca gggacagcgt atcgcaatcc tggagaatga ttttggcgct
      121 gttaacgtag atatggtgat gctgcaagac ttgaaatgtg agaactgcca gctggaaatg
      181 attgccggcg gcggagaccc ctgctgtcat aaacgacgct ttaagacgca gttgatctcc
      241 ctgggaatgc agcattttga tcgagttatc atagaaccct ccggcatctt cgacatggac
      301 gagttctttg atacgctgta cgagtcgccg ctggatcagt ggttcgagat cggtagcatc
      361 ctaaccattg tggatgctga aatggatgat gtcctttcag cgcagatgga gtatcttctg
      421 gcatctgagg cagcctgctg tggcaagctg attttgagca agtggcagaa tataaaaggg
      481 gaatccgaag aagaccttgt ttcccacatt ctgacccatc tgaaccgctc tttgacaggc
      541 atccagtgta aacgacagtt ccagccgaaa gacgttctga cggcggactg gagcgctctc
      601 cgtgcggagg atttttctgc catgcagtct gccggctatc gcaattgcag ctatgtgaaa
      661 cagttcaaca cggagagcat ccaaagcgaa gtccattact tcatgcacat caatttgccc
      721 gaaaaccaaa tcgaaccgtt gatacagcaa attctggcgg atgccgcctg cggtaagatc
      781 taccgtataa agggcgcatt gccggcagaa aacggcgact ggctgaaagt aaacgcaacc
      841 gcagagaaaa cggagatctc accagtcaaa gacggccagg cagttctgat tgccataggt
      901 gacaatctgc accgggagaa aatcgacgcc tatctgcaaa agtataacac cgatccggaa
      961 tatgtttcta tataaacgaa aagccgcagg caaacccata aaacggtttg cctgcggctt
     1021 ttgtgctatt ctgtgtgatt actgatagtg tgccagaaac tgcttggtgc gctcctgcgt
     1081 cggacgttca aagatctgct ccggagcgcc ttcctctaca atgtaaccgt ctgccataaa
     1141 gatcacacga ttggaaatgg ctctggcaaa gctcatttca tgggtaacaa ttaccattgt
     1201 catgtgatcg tctgccagca gccggatgac ttccagaatt tctccggtca gttccgggtc
     1261 gagggcagaa gtcggctcgt caaagaataa aatcgaggga gacatcgcca gcgcacggac
     1321 gatcgccaca cgctgctgct gtccgccgga cagttcacag ggatatgctt tcgccttgtc
     1381 tgccagcccc acctttttca gcagttccat tgccgtctgc tctgcctctg cacggctttt
     1441 tttcagaatt agccgtggtg cctcggtaat gtttcgcagg acggaatagt gcggaaaaag
     1501 gttaaagttc tggaatacca acccgaagta gtgctggatt ttgcggaact ctgtcttcgg
     1561 cgcatagacc ggacgcttgc cgtcctcagc gctgactgca agctgatcca tgtacgagat
     1621 cttgccgctg gagagggtct ccaggaaagt gcagcagcgg agcagtgtgg attttcccga
     1681 accggacgga ccgatgatcg aaacaacttc tcccggcttt acgaaaaagg agatatcttt
     1741 caaaacctgc acgtctccga aatgcagttg caggtgttca acttctaagt aattcaatgc
     1801 ggcacctctt aaattttgta gtagttcaat ttcttttcca gcagagacat cagaccggca
     1861 acgatcaggt taaaggcata gtagaaaatt gcagcagcca gatagggcag catagatgtt
     1921 tctgcggcag cgatcatttg tgtttctgtg aacaattcga tgtaagaaat tgcaaatgcc
     1981 agggaagtgt ccttgaccag tgtgatagac tcgttggtca ctgccggaag aacacgcttt
     2041 atcatctgcg gcgtaataat gcgcaggaat ttctgcatcc ggctgtagcc caggatcttt
     2101 gcggcctcgt gctgtcccac cgggatcgac tgaatgccgc tgcggaaaat ttctg
//
LOCUS       DAWENO010000246         2144 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15545_length_2144_cov_9.033030, whole genome shotgun sequence.
ACCESSION   DAWENO010000246 DAWENO010000000
VERSION     DAWENO010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15545_length_2144_cov_9.033030"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1799..1808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          500 a          541 c          570 g          523 t
ORIGIN      
        1 aaagcaaagt gaagatgatg ctgtcccgga cacgaaaaga acttcgggat tatcgggaaa
       61 aggaaggtgt tgaactatga aagagtttcg gcttttgtat ctcattggag acatccagga
      121 caagtttatt ttagaggcgg cacaggaaca gcaggacacc aggcgaggcg gattttcgaa
      181 aaaaatccgg tattgtgctt tgggactgtg tgctgcaatg gcactgtgtg ccggattggt
      241 ggtatatacg cagttccagc gcacttcgga cagtttggta ggccgtccaa aggtgactac
      301 cgcagtgaca gatgacgatg tgcagatcgc aaacccattc caggaatgca ccgacctttc
      361 cgaggcggca cagatcgccg ggttctccat tacagcaccg gattcctgcg tcaacagttc
      421 tggtaaagtt atacaggcga tacagaatga tatgattgag atcatctacc aggacgacgc
      481 cggcaatgaa ctggcacgaa tccggaaagg gcttggcacc agcgaaatca gcggagacta
      541 taacttatat gagaacgatt ctacccagga aattgacggc aagagtgtac acattcgtgg
      601 aaacggcaga ttggtctatg ccgcagactg gacagacggt gtttatgcgt attctgtgac
      661 atttgaatcc ggcgcagcgg aagacactgc ggaagatgtt atcatggctg tgtattaaaa
      721 tgcaatgcta tctcctgttt ctgtatgctc ccttatgcag aaatgtgata taaaagatac
      781 ctcctgcttt tctctgagca ggcagtatct gtgcaaggtc tgaaaagacc gcttcctcct
      841 ttattacagc gttctgtttt caaggcaaca ccgcataaag cttgtgcgta agatgcgtag
      901 ttcgattttt cggcagactg tatagaaatt tcgcaggcat actgtcgtat gtcaaggaat
      961 ttctgtgcag tatgacggga aatcgacaag cagattgcgt acaaagccaa taggcgggct
     1021 ttgtacggtg ttgcccttcc cccattgaaa cagaacgctt ttttatttcc cattccggaa
     1081 caactttccc aacgcctccc gaatctgtga cagggcatct gcggcagtgt ccggttgaaa
     1141 ggttgtttca tagtcgtaga cggcgacgcc gtccgttccg tttgtttcca gcgccagttt
     1201 tgcctgacga atgggcagcg tcggctcact gcaccactcc atgctgccgc tgcctgccca
     1261 ggtgtcctct ttgccgcact tgtgcgtgcc gatgccgatg accagagaaa cggactcgct
     1321 ggaaactgtc tctgtccagg tttgcagcgt ctccgcaaaa ggtgcggttt cgttggcaaa
     1381 gccgtagtac agttgcggaa tcagtacatc gcagtagccg gtttccgtcc cccagcggcg
     1441 cacgtcggca tattgtttcg tgtagttgcc gtccatcgtt ccctgcggac tgatactcag
     1501 ctgtattgcc ggagacgcag ccttaacggt gctgtagatc tgctgtacca tggcattgac
     1561 ctgttccagc cggaatgcgc ccagatcgtc cgcttttgca tcggcaaatg cagctgcatc
     1621 aaaggcggtt tctgtggtgg ggtagaagta gtcgtccaga tggatgccgt ccacatggta
     1681 ttgcgccaga atttccgcca cgccgtctgc caccagctga cgtacttccg gatatgccgg
     1741 gttcagccac cagtattctc ccactttgca cagataggtg ccgttcttct gcggatcgnn
     1801 nnnnnnnntt accggatagc tgtccgacat cgatgccatg gcggaatcct tttgcagccg
     1861 tagcggattc acccagccgt ggacggacaa atgcagctgg tgcgcctctt ccagcagaat
     1921 ggaaaacggg tcataatcgc ccacttcctt tgccggcgga aagagggcag attgataata
     1981 ggcgtctccg aatgcccgga gatggagata gactgtgttg atccccatgt ctgccacctg
     2041 ctgaaagcgc tggcgcacag catcacggaa ctgatctgcc gtctttccgg caagaatctc
     2101 cccgtagtcc agatagggaa accacatccc gtataccctg tcat
//
LOCUS       DAWENO010000247         2142 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15570_length_2142_cov_7.706277, whole genome shotgun sequence.
ACCESSION   DAWENO010000247 DAWENO010000000
VERSION     DAWENO010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15570_length_2142_cov_7.706277"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          408 a          645 c          536 g          553 t
ORIGIN      
        1 tcctgaaact tcttgcgaat gttttccttg actttttccg gatcgttccg gatccatttc
       61 atatcaatca tgattttttc cttcctttat cttatgtggg atgcccacaa gattacagta
      121 tgtagggcga acaatgtttc acgtgaaaca acctcgccgc cttactctct tgtcagtttc
      181 tgcgccagtg cagacaggtc gtccttgtcg tagaactcca ggttcagcgt gcctttgttc
      241 ttgccagcgg tcacggtgac acgccgtccc aggcggcttt ccaggctgat ctcgatttcc
      301 ttgtaaaagc tgtccttatg agaaaactgc ggcatttttc cgggcttttc cccgtctgcg
      361 gcgatcttct ccaccgcacg caccgtcagc ttgccctctg ccgcctttgc cgccgtttcc
      421 gagagcagtt tctcgtcgga aatggccagc agcgcccgtg cgtgtccggc gctcaggtcg
      481 ccgtcctcca ccagcttttt caccttctgg ggcagtttca gcagccgcag ggagtttgcc
      541 acagcgctcc gggattttcc cacacgcttt gccacttcct cctgagtcag ctggaacatt
      601 tccatcagct gtgcataggc gttcgcctct tccaccgggt tcagatcctc acgctggaga
      661 ttctcgatca gagcgatcgc catggtctcc tcgtcggtca gctcccggat caccaccggc
      721 acttcgtcca tgcccagcat tctggcagca cgccaccggc gctcgccggc aacgatctga
      781 tagttcagtc ccagcggacg caccagaatg ggctgtatca tgccgtactg ctgaatggaa
      841 tccgccagcg tctgaattgc ctcatcgctg aactgtttcc ggggctgatc ccggttgggt
      901 tccaattctg acacacgcag ggtctttttc acctggacat ccgctgcatt ttcatcgtag
      961 agaaagtcca gaccgctccc cagtccgccg atcgtcatag gtcatcacgc tccctttttc
     1021 tgccgaatgc aagcatccgc cgctttttct ccggcggctt ttctcccagc agctccagcc
     1081 ccaggagcat atacgcctcg gcgccacggg agagccggtc gtagtacatg acgggctttc
     1141 cgtggctggg tgcctcggaa aggcgcacgt tccggggaat cttggtctga aagacctttc
     1201 ccggaaaata cttcttgact tcctcttcca cctgccggga cacattgagc cgctggtcga
     1261 acatgacgaa cagaatgccg ccgatgtcca gattggggtt atagttgctg cgcaccacct
     1321 taatggtatt ggtcagctgt gacagaccct ccagcgcata aaattctgcc agcatcggga
     1381 taatcagctg gtcagccgcc accagtccgt tgacggtgag cagccccagc gccggcggac
     1441 agtcgatgaa gatatagtcg taatccagct tacaggtcag cagctgcatt ttcagccggt
     1501 tcatccggtt gtccatattg gacagctcca gctctgcccc ggcgagggtg ttctttgccg
     1561 gcagcaggct gacgttttca aacgccgtgc ggacgatggc ttcctggatg ctgcactttc
     1621 ccaccagcac ctcatacgat gaggcgtgga gcagtttttt cttgatgccg agaccggtcg
     1681 tggtgttgcc ctggggatcg atgtccacgc aaagcacacg cttgccacga gagccgagat
     1741 aagcgcccag attgaccacg gtggtggatt ttcccacgcc gcctttctgg ttcgctactg
     1801 catacaccat actcacgctg ttttctcctt tcctgcggtg tgccggacgt gggatttctc
     1861 cccgtttttc ccggagcgca ccgtgcatga cgcacacaaa tactctttca ttataacacg
     1921 attttccgcc cttgtaaagc attttccgga atttttgcgg aatgtctgtc ggattttgtt
     1981 tcacgtgaaa cattttccat ggaaacgccg gcaaaaaaaa attatcgcaa agcatgacac
     2041 gatcccccag cgaatgcagt cgggtttttg ggaaatggtg tcggctgaaa gcgttatttc
     2101 ccaaagcggc gctgaaaatg cccgaaacag gcggattttt tt
//
LOCUS       DAWENO010000248         2116 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15791_length_2116_cov_5.374575, whole genome shotgun sequence.
ACCESSION   DAWENO010000248 DAWENO010000000
VERSION     DAWENO010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15791_length_2116_cov_5.374575"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          422 a          546 c          542 g          606 t
ORIGIN      
        1 tatatttctt gtgaagacag gttctatgag gcagaacgtt tctgcgtgcg gagaaaatca
       61 cacagcgctc gttctgtcaa ttgggagaaa aattggaagt tgttgtagaa gttatcactg
      121 gctgttggat atgtttactg taagtcgccc agggaaacta tcgttttctg accgtagccc
      181 aggatatcag catacagatc ctgcaattcg tccgcatcca tgaggaatgc cacagttgcc
      241 tcaacggttt cgcccggtgc aacattggtg atgttgttct tgctgtggtc tctgtcagtg
      301 gtaaacagga agaactcacc atacttcaag tcgtccaatg tgccattgca gtacatggtt
      361 tcatccgttg gctcgcaggc aacttcgccg taatccagtc tgtcaccgtt cttctggagc
      421 agattcgggc aaacgcagat ctcctgcgta atgctgctct cgttggtgta gctcaggtgc
      481 atcacaagga cacgctgggg aaccgtctct tccttgacct tttcatccag ggtgttcacg
      541 ccgtcgccac gggagtacca tgtgcgcaca tcatccttga ccgtaccgtc tgcattgaga
      601 tactggctgt agtccaccgg gttgccgatg tcatccactg ccggaagtcc gtcaaagtta
      661 tcctggacgg aaacgctgtc cagcttgaca gtgacgcggg aatatttatc atcatcatca
      721 tagaaatccg aacgaatggt gtcaccgatc tgctgaatgg tatagtcatc gaccttgtag
      781 acctcatcgg ttccgccagt cgcttcttcc tgcggctcac cgctccacag ctcagctgtc
      841 tccttgtcag aaggtgtcag actgagattt tctgcgatct tttcgatctc ttcctttgtc
      901 acgccggttg ttgcatagaa ctgtacggca tagttggaat ccgggaatgc gacccagaca
      961 tccttgtcga aaatatcctc gccgaactgt cctgtggtgt cagtcaccgc atagagaaca
     1021 gtgttattgc cgcaggtgag cgtctcctgg gaaacgctgt agctgacggt gtgcgtcaga
     1081 gcatcgtcct tgttgtccat tttgtagaac gacatggtta tgccacgacc gttgggatcg
     1141 ctgtattttc cgctgctggg atcgatcgcc attccgtcgg gcatccagcc gacctgcagc
     1201 gccatgatct ggtcagaaac gctgtcgctc ttcgggatca ccatttcctg ctgatactcg
     1261 ccgccgccgt tctggaaata gccacggatc tgggtggatg cgtatacggc agtcgggatg
     1321 accacgatgg ctgcggcaac tgctacggct gcggtcagag atttcttcat gcggaattta
     1381 tgctgtttca tatgagattc ctccatctgg cgccggaatt cctgcttccg gctggtgtag
     1441 cgttccgaaa agacatgggg ctgtgcatcc ggtgcaaagg catcgtctac aattttattc
     1501 cattcggttt cgttatacgg aaaagagtcg tgtttcatag caaatatccc ctttcttttt
     1561 ctgaagcgcc ttcttggcac gttcaaagcg ttttcttgca gcagcctcct ggatggagag
     1621 ccgctcggca atttcccgga aagacagctc ttcttcgcac cgcatgagga tgatctgccg
     1681 gtccgtttca ttcagcggtt ccagaagttc tgcgatggtc tcttcttctg tgatgtttgc
     1741 gagcagctgt tccatgtcgt ctttctggct gggtacctgc aaaatgcggt cgtcccacac
     1801 ggtatagcgt tccgaatccc gctgattctg ccggtattgg ttgatcgccg tactgcgaat
     1861 gatacaaacg atatagtgct ttgtcttttc gctttgtaca ttgccgatct ttttcagatt
     1921 cttcatgatt cggagaaacg catcggatac agcatcttcc gcctgttctg tgtgatgcag
     1981 gattgcgtaa gcgatgcggt acatgggctg ctcgtagagc gcatagagct gatcgagctt
     2041 ttcctggtct ttgttcgctg ccatagtttc acctctttct gagacgggct gccgttcctg
     2101 tgcatcggtc tttcac
//
LOCUS       DAWENO010000249         2111 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_15819_length_2111_cov_7.474222, whole genome shotgun sequence.
ACCESSION   DAWENO010000249 DAWENO010000000
VERSION     DAWENO010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15819_length_2111_cov_7.474222"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          470 a          563 c          503 g          575 t
ORIGIN      
        1 atacgataaa tattctttgc aagcgcctgt ttttccagca ccggcgcaag catctgttca
       61 tatgccatga gtttttctcc tttcaagcaa aaatggcggg cgggaaatct ccccgtccgc
      121 cacggtttgc gtgattttca aattatattc cgctgtcaga tcttggtgat gtctaccatt
      181 tctacatcat caatgctgcg gccggattcc agcacgtttg ccagagcctt tgccgtgtcg
      241 atcgctgtca gacagcagat cgaacgctct accgacttac ggcgcatctt tacgctgtct
      301 cttgccggga gacgaccctt tgcagaggta gagatcatat agtggatctt accgctttcc
      361 agcagtgtaa tggtattcgg ttcgccgtca gagatcttct tgaccaggtt agtggcgatc
      421 atgttgtgat tcagcacagc cgctgtaccg cttgtaccgt acagttcaaa gcccatgcga
      481 gccagcttgt ctgcgatctc aatgatttcc tgcttatcgc tgtcacggac agagatcagc
      541 acgccgcctt cctttttcag atcatagccg gctgcaatca aacccttgag cagtgcatct
      601 tccaggttgg ttgcaatgcc caggcactca cctgtggact tcatttccgg tccgagcatg
      661 gtgtctacgt cctggagctt ttcaaagctg aataccggaa ccttgactgc aacatactta
      721 cccttcggat acagaccgga cttgtagccc agactctgga gtgtttctcc cagcatgatc
      781 ttggttgcga tatcaaccat aggaatgccg gtaaccttac tgatatacgg tacagtacgg
      841 gaagaacgag ggtttacttc gattacataa acttcgttat tgtataccac gtactgaatg
      901 ttaatcagac caacagcgtg gagttccttc gccagcagac cagtatagcg aacgatggta
      961 tctgtgatct tttcgctgag gttctgtgcc ggatagatgg aaatggagtc accggagtga
     1021 acaccggcac gctctacgtg ttccatgata cccggaatga ggaagtcctt accgtcgcag
     1081 atggcgtcaa cttccacctc agtacccatc atatatttgt cgatcagcac cggattttcg
     1141 atcatatggc tggtgatgat tgccatatat tcttcgatat ccttatccga atgcgcaata
     1201 atcatattct gaccgcccag aacgtaagac ggacgcatca gaaccggata gcccaggtcg
     1261 ttggcagcct tgagtgcctc ttcggttgtc atgacagtct cgccggcagg acgaggaatg
     1321 ccgcatctgc ccagcagctc atcgaaacgc tctctgtctt cggcagcgtc gatatcatct
     1381 gcggaagtac ccaggatatt gacacccata tctgccaggt agcgtgtcag cttgatggca
     1441 gtctgtccgc cgaactgtac cactacgccg tacggctttt cggtgttgat gatattggca
     1501 acgtcttcct tggtcagcgg atcgaagtac agacggtcgc cggtatcgaa gtctgtggaa
     1561 acggtctccg ggttgttgtt gcagatgatt gcctcacagc tcatctcttt cagtgtccat
     1621 acacagtgta ccgaacagta gtcgaactcg ataccctgtc cgatacggat cggaccggaa
     1681 ccgaatacga taatcttttt cttgtcgctg tgctgacgtt cgatgaactc ttctgcttcg
     1741 ttttcgtcgt cataggtaga atagaagtac ggtgtctctg ccttgaactc gcctgcgcag
     1801 gtatcaacca tcttataaac ggcatggcgg tgcatcgggc atttctgacc ggaaatacgc
     1861 tcaatggtgg catccagata gccgtactgc tttgccagca ggtacagatc ttcggtcagc
     1921 ggcttggcag ccatttcccg ttccagttcg atcagattca tcagcttgtt taggaaccag
     1981 cagtctacca tagtcagctc gtgcagttct tccacggtct tgccacgttt cattgcctca
     2041 tacagctgga atgcacgctc atcgtctacg acctggagac gctccataat ttcatccaga
     2101 gacatcttct c
//
LOCUS       DAWENO010000250         2078 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_16084_length_2078_cov_9.382106, whole genome shotgun sequence.
ACCESSION   DAWENO010000250 DAWENO010000000
VERSION     DAWENO010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16084_length_2078_cov_9.382106"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          516 a          500 c          621 g          441 t
ORIGIN      
        1 ggcagactgc cggattgtgt cgttgcctgc gtcggcggcg gctccaatgc aatgggtatg
       61 ttctacgatt tcattccgga tgagagcgtg cgcctggttg gtgcagaggc tgccggaaag
      121 ggaatcgaca ccaaactcca tgcagcaacc gttgccaagg gcagcctggg tattttccat
      181 ggcatgaagt cctatttcct ccagaacgaa gagggacaga tcgctccggt ttactccatt
      241 tctgccggac tggattatcc cggcgtagga ccggaacacg caaacctcta taagaccggc
      301 cgtgcagaat acctgcccat cacagacgaa gaggcagtgc aggcactgga atatctgtcc
      361 aggacagagg gtatcatccc ggcaattgaa tcggctcatg ctgttgccgc agcaatgaag
      421 atcgcaccgg agatgaaacc ggatcagatc atgattatta acgtttccgg cagaggagac
      481 aaggatatgc agcagatcgc agaatacaga ggagtgaaga tcgatgacta agaacagaat
      541 tgatacaaaa ctggcacagt gcaaggctga gggcaaaaag gcactgatta catttatcac
      601 tgccggagac ggcggctatg actgcacaga aaaggcagtg ctggaaatgg tagaaaacgg
      661 cgcagatctg atcgaactgg gcgtgccgtt ctctgacccg attgcagaag gtccggtcat
      721 tcagtgcgcc agcgagcgtt ctctgaaaaa tggcacaact ctggtaggca tcttcgagtt
      781 ggtgcgccgt ctgcggaagc agacagatgt gccgctgctg ctgatgatgt acctgaacac
      841 cattttctgc tacggcacag accgtttctt tgcggagtgc caggaaagcg gcattgacgg
      901 tgtcattgtt ccggatatcc cgtatgagga aaaagatgag attcagggca gcgctgacaa
      961 atataacgtc cataacatca gcctggtaac accggcgagc gaagaccgca ttcagatgat
     1021 cgcatctgag gcaaccggtt tcctgtactg cgtatcctcc aacggtgtga ccggtgtgcg
     1081 cagcgagttc gacaccaact ttgcaccgtt cttcgagaag atctataagt acgcaaaggt
     1141 accctgtgcg gtcggtttcg gcatttccgg tgcagaggca gcaaagcaga tgagcaagta
     1201 ctgtgacggc gtgatcgtgg gcagtgcaat cgtgcgtcag gtcggcgaat acggcggcga
     1261 ggctgctccg gaaaagatcg gcgcatttgt caagagcctg cgagacgggc tggatgtgta
     1321 agcgtcatcg gtttttttca aaaaaagatt gacaacccac cggaaatctg ctatactgta
     1381 agtatagatt tatcagcgat gaacggagtg actgctgcgg aggctgcaca gagagggcgt
     1441 gtttggtgga aacgccacgg ctggggcgca ggtgctgtcc gggagctgtt ccgggaaacc
     1501 ggaacgtatt tcctgcgtta aaggagcatg agggaagtgc catggcactt ctgaacttgg
     1561 gtggtaacac gaaggctttc gtcccatttg gcggcgaaag ccttttttgt atatccgggg
     1621 tgtgtctgtg gcgcattccg gaatagtgaa caggagggaa cgcatatgct gccgatggat
     1681 tgtcacaccc acacgagaat ctcgcccgac ggatcgggga cagttgccgg acatatcgca
     1741 caggcggaaa agctggaact gccggtgctg gcactgacgg aacacgtgga gatgaaccgc
     1801 tatttcccac aggctcatta cgggacagaa ccacggaacg agtgggagat cttcgaccac
     1861 gccgccgtgc tggaacagtc cctggaagtg gtaacagctg ccaaagaaca ggctgccgcc
     1921 aatggtctga ccctgctttg cggcatggaa atggggcagc ccaacgcaga ctttgggctt
     1981 tcagaatctg tgagccggga tatgcggctg gatttcatta ttgcatcgct ccacgaacta
     2041 ttggataagc cggatttttt ctgcctggat tatacgca
//
LOCUS       DAWENO010000251         2072 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_16135_length_2072_cov_11.297967, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000251 DAWENO010000000
VERSION     DAWENO010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16135_length_2072_cov_11.297967"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          585 c          510 g          550 t
ORIGIN      
        1 gcctcgcagt tttcaaatgc tgctgcaccg atacgggaaa cggtgaccgg cagcgtgatc
       61 tgtgtcaagg cggtacagtc ccggaatgcg tgatacgcca cacgagtcag agaaaccggc
      121 atggtctctc cacggagaat cacgctttgc agagaactgc acccctcaaa tgcacccacg
      181 ccgtctgcgc cataggaaat ttccagcagc gtctccggca gctttacggt ttgcagggtt
      241 gtattcccgg caaaagcccc ttggatcacc tgcaccggaa gcccgttcac cttttccgga
      301 acagtaacgc tttcagaatc tccggtgtat gcggtcagcg ctgcatactt gtcgaacagc
      361 cggtaagtat aatcaccgtc gagatagacc gttccgggct gcaggtcgta atctgcgtcc
      421 ggaacatccg gcaggtcgta gaggaattcc caaccgcttt tatcgtagac atacggggaa
      481 tggttcttgg aaaagctggt ctcaccagcg gcaaaatatt tgctggtctg cgctgtatcg
      541 tcccaggtgg catcgaaata atagtaataa ccgtcgtcca tctgcgccat attccaggcg
      601 tgaccgccgc cgttccccaa ccccacaata tagaagttgg agaccccggc tgcgttcatc
      661 agcagctgga acgacttggc gtatccctcg cagactgccg tgtgatactg tgcatcaaac
      721 acgccggtta tgctgtggga agtcatggag ttatccggat cgccgttggc atcataggca
      781 tatgtaatgg tgtccaccag ccaatcgtgg gcgattctcg ccttttccag ggctgtttct
      841 ccggctttga ctttcgtttc cactgtcacg atctggtttt ccagaacctt tcgctccgcc
      901 tgccgctctg tgccgtcggt gcagttctct gcacaggaaa gataaaccat atcaatataa
      961 cttttccctg tccagaaatc ataatccgtg ctgtacagga acgaactgcc cagaaagaaa
     1021 taaacgggat tgtcgttccg gaacatggaa atcgctctga ccacgtcttc tttattcaga
     1081 gactggctgc cggtgtccac tgccacacag caatccatgg tgcctgtgga ataggaaaca
     1141 ctttccgcat ctgcgcttcc cacgtagaag gtatgcgccg cctgtttcat cgcattgtac
     1201 agctgtttct gttcatcgct cagcgtatcg tacccggcgt gcgacgcata gcggtcctct
     1261 gcataagctg tcgccgtgct gttgtctgcc ccgtaaacgg gaatcctttc cgtctgcacc
     1321 gcagctgcgt cggcatccag cgggatcccc tgcatttcca acaggtctcc cgttacattt
     1381 tccccggacg ctgccgctcg gacagagggc gatgctgttc ccgacagggc tgcgacagca
     1441 aaaactgcgg cacacagcaa tccggtgcat ctttgtattc tgtttgtctg catcttggaa
     1501 tctacacctc cgtttcaaga ttttccatct tttttattat accatattca cgaattccct
     1561 gtcaatcttt ttgtggattt ctgtgaaatt taccaatgcc tttcagcttt tcttttccac
     1621 aagtaatacc acatcggtgg aaaaagcggc agagaaaact gttcctctgc cgcttttcga
     1681 ttactatttc aacgatttcc ggaactcatc ctgccatgag cctgctccgc agcaagcata
     1741 ggtcgaaacc attcagcgtt ccgtcagtat acagatctcc tgcctgccaa tcggcaagtc
     1801 tggtctcggg aacgcccagc agccactttt gcagcagcac cagatctgcc aggtttacct
     1861 ttccgtctgc gttgatgtca ccggaaatcg caggcggttc tgtggccgtt gccgttgtgg
     1921 atactgttgt tgtatccgtt gttgctgcag tggtcgatgt tgtcgttgcc gcagtcgttg
     1981 ttgtggtcgt agtggtagtt gtggttgtgg ttactgtagc ggtctctgtc tttgcctgcc
     2041 atttctgaca gctgctgcca gtcggttccc ac
//
LOCUS       DAWENO010000252         2071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_16148_length_2071_cov_7.883433, whole genome shotgun sequence.
ACCESSION   DAWENO010000252 DAWENO010000000
VERSION     DAWENO010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16148_length_2071_cov_7.883433"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          601 a          478 c          624 g          368 t
ORIGIN      
        1 atctccgccg gggtgcgtga gccggtgatg cgtgtgggca atctgacagc ggcacgggat
       61 ttcaccgatg tccgtgatgt ggttcgggcg tattctctgc tgatcgaaca cggacgctcc
      121 ggcgaggtct acaacgtagg cagcggcaga gccatttcca tccaggagat tctggaccgc
      181 atcattgcac acagcggcac aaacatcacc gtggaagtag acccggcgaa gctgcgtccg
      241 gtagaagtgc cggtgatccg ggcggatatc tccaagctcc aggcggacac aggctggcag
      301 ccggagatcc cgctggaaca gaccattgca gagacactgg acgactggaa aaacaagctg
      361 aaataagatt ggaggaggcg caggcgtatg gcacaattgg aagaactgct ggaaaagaaa
      421 ccggatgctg tgtctgagga tatgggatac agcacccctt ctgacgcata tccggaggac
      481 gaatgggaaa agcagaagct aaagggctgg cctcgccaga tccatgtgtt cgggatgcgg
      541 cagaaccaga tcaaccgtgg cacagcggct cggctgcgtg cgctggaaat ccagaaccgg
      601 acgctggaga tcaagattca gcgcctggaa gaagaactgg agggcctggc ggcacgggag
      661 aagaagatcc tgggcagagt tgcggctgaa atggacacca tgcaggcaga gatcaaccgt
      721 ctgcggctgt ccacccagct gaacagcaat gcgctggacc gcattctgcg gcagccggaa
      781 ccggaaagcg cagagaaaga aaacgattcc caggaatcgt aattgataca gaacagcggc
      841 ggcaggcact gcaaaatggt gtctgccgcc gttttcgtct gtaaacactt tgtaggattt
      901 tgtcggaaaa gtcaccgcat tttgcgaaag catacacgaa atgcagcaga aaaactaggc
      961 aaaatgccgg agcagcggct gcttttgtaa atgaattgta agatttccct tgacaatcgg
     1021 aggaaagtac gatataataa tagaaagtgt tttcgcagaa atgccggaaa caccgcttaa
     1081 aatcagaaca ttgtacatga atatgacggc agaccgggaa cgctccggga tgcaataacc
     1141 atgacggagg aacattatgg gcattttttc aagaatcggt gacgtgctga aggcaaatat
     1201 caacgacatg atcgaccggg cagaagatcc ggaaaagatg gtgaaacaga tcatcctgga
     1261 tctgcaaaag gaagtcagca aggcaacaca ggcgctgggc aaggcaatgg caagcgagcg
     1321 cattgcaaag cggcagtatg aggatgccgt aaagacctcc tccgattggg aaatgaaggc
     1381 aaaggcagcg ctcaacgccg gcgaagtcga tctggcgaag aaagcccttt ccaataaggt
     1441 caaggcagac gaaaacgtca agcagtatca gcagatgtat gagcagatct cggcgcagac
     1501 cgcacagctc agagagcagg tcgaggcgct gaaatcaaag ctggacgagg cgaagagccg
     1561 ccaggcaatg ctgatcgcac gttctcagat ggcaaagacc cagaagaatc ttgcacagtc
     1621 tgtgggcggc atggatgcct ccagtgcgct ggataaattc aaccgcatgg aagaaaaggt
     1681 gacccgccag gaggctgagg cacaggcatt ctcggaaatg gctggtgtta ccggctctgc
     1741 ggaggaaaag tcattcgaac agttacagca tgatatggac gtggatgcag agctgcagcg
     1801 gctcatggca gaaatgggtc agacagaaac tgcatcggaa acagaaggga gtgcagttta
     1861 atcatggcac ggaaagaaga aaaaacatct ctttgcggaa ttctgatggt cttggcggca
     1921 atcgctgtgg gcgtggcagg tattgtgtat gcgctctcta cgcaccgccg ccatatcaac
     1981 cgggaaaaat ggaaggacta cgacgagtgc ggcttggtat aagcggcaga tttctatgaa
     2041 aaatggaatc acattccatc gtaaatatcc a
//
LOCUS       DAWENO010000253         2060 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_16242_length_2060_cov_9.663840, whole genome shotgun sequence.
ACCESSION   DAWENO010000253 DAWENO010000000
VERSION     DAWENO010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_2060_cov_9.663840"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          488 a          498 c          607 g          467 t
ORIGIN      
        1 aaacgaattg cattgcggaa aaaggtgtgt tataataaca atatccaatt ggaaaaaagg
       61 ggaagtgaat atgcacggaa ataagatcgt gctggacttg gagtttacac cggttcgtga
      121 cccggacctc caggcagttg cgagcagcga gatcttagag atcggtgctg tcaagttaaa
      181 tgaaaacaat gaggtaatgg aggaatttca aacctatgtg aagccccagt acagcagcat
      241 tccgccgcac atcactcgca tcaccgggat cacggagaaa acggtggaga atgcgccgtc
      301 ctatggcgca gcggtggcgg ctttcacgga ctgggtgggg gacagcgtgc gcagccggtt
      361 ctacacctgg agcaattcag accagggcgt tctgttgaaa gaggcggatc tgaaagagca
      421 gacgctgcac aaaatgttct atacctactg ggtggatctc cagcggatcc accagcggct
      481 ctacgggttc acccgtccca tgaatctcac cagtgcgctg gggtcgatgc agatctattt
      541 caaggggacg gagcatggtg cgctggcaga tgccagaaac accgccggaa tcttgcagca
      601 gctcagccat gtacaggcgg tgcggcagca gatcgaggca tcgcacatta catacaacag
      661 cacagagtcc catggattca ctatggggct ggttttcaag aaccgctgaa cgcaaaaaag
      721 cagagacacc ggatgtggta tctctgccgt aaaagggatt cgcataaaag ggattcgatt
      781 gttttcggtt acgggcgctg acccagaccg ccttccagtg taatggtctc gccggtcatg
      841 aacttgaagt ccggagatgc cagctgaacg cagactctgc caatctccag ttcagcgtcg
      901 cccaggtgac ctaccggcgg catcttgacg tttttctcaa atgcgtccgg ataagctttc
      961 tggaagtttt ccagctgtgc ggtccatgcc agcgggcaga cgatattgac gttgatgttg
     1021 tctctgcccc attcggtagc ggcaacacgg gtcagaccac ggatgccctc tttggctgcg
     1081 gcataggcgc actgtccgaa gttgccgaac agacctgcgc cggaggcaaa gttgataaca
     1141 gacccctggg tctttttcag atagggatag caggctttca tgtagtggaa ggtggcgtac
     1201 agaccggagt agatcgccag gtcaaactgt tcggtggtgt ggtctgccag ggtcacgccg
     1261 gaggcagatg cctgggcgtt gttgatgagg acgtggatat ctccaaaggt gtccactgcc
     1321 tgcttgacta cgttttccac tacagcggcg ttgtctgcgc cggcattgac gtccgcctgt
     1381 acagtcagca cctgaatgcc gtagagccgt tctagctctt cttttgcggc gtccagcttc
     1441 tggacatttc gcccggtgat gaccaggttt gcgccttctt tggcataggc ggtggcgatg
     1501 ccgtagccga tggagccgca tctgccgtca ctcagaacgg ctctgccggc gccggtgata
     1561 attgctgttt tgttggttaa aaagcccata agaaacatcc tttctggttg atgggttagt
     1621 acttgctaat ttcagtatag catattttgt ttcaaaaatc aagaacgatc tttgggaaaa
     1681 ttcgaggagg agttatgatg cagattcgaa aagcgatgag tcgggacctg gagtgcatcc
     1741 gggagttgac gagagagacc atcgggcaga tctacccgaa atattatccc cagggcgcag
     1801 tggaattttt cctgtcgcat cattgcgacg gagcgatcct ggcagatatc cgtgacggtg
     1861 cagtctatct gggtattgac gaaacagacg gcattgtggg aacagtcacc gtcaagaaca
     1921 gcgagatcag ccgtctgttt gttctgccgg aacagcagcg gaaaggatat ggagaggcac
     1981 tgcttgccta tgcggaaaag ctggtgtttg cggagtatcc ggcggtgacg ctggacgcat
     2041 ctctgccggc aaaggcaatg
//
LOCUS       DAWENO010000254         2059 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_16257_length_2059_cov_5.737525, whole genome shotgun sequence.
ACCESSION   DAWENO010000254 DAWENO010000000
VERSION     DAWENO010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16257_length_2059_cov_5.737525"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    695..704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          571 a          440 c          530 g          508 t
ORIGIN      
        1 cggtgatacc tgggatccga caaatgactg gagctatcag ggcattacca aatgcaagga
       61 cacataccag gatggttctg agaccagaac agactatatc tgtgtttact ccaacggaga
      121 gcttgtcggc ggtattgaac cgaacggcaa caagccggct gtaacaacca cgccgtcgga
      181 aacgacaaca ggcacgacaa caactactac cacagtaaca acaacaacaa caacgaattc
      241 tgcaaccagc acggaaaaag caacagaaac aacagtttcc acagaagaga ccacgacaat
      301 gacatctgaa acgacagaga caactgcaac aaagcagact tctgaggcag cctctggttc
      361 gtctgatgct gccggctcta ttctctacgg cgatgcgaat ttggatggca gaattgatat
      421 tacagatgcg gtactgctga acaagattgt tgccggctct gtttctgcca atacacagca
      481 gcggttaaac ggcgactgca atggtgatgg cgcattggat tccggggatg gtatcctgct
      541 gctgcaattc ctggtacaca ttatccagag cattggaccc agcgcaaaat aaaggaaagg
      601 aatcacaaga tacatggaaa tttcaaatcc ggagaatcat tctgggaatt ggcaggaact
      661 gcggcagatt ctgaaagaaa agcaatttgg gacannnnnn nnnncaaatg ggatttcttt
      721 tgtggcagga ttgcttgcaa attacttttt gagtacattc tgggtctttc gctcttctaa
      781 agtgcagtct cggttggttg aatttcttgc gtttgcagct atcggcgtag tcggattgct
      841 gctgacgctg ggaattacca aactgagtga gatctctctt ggaagtgtta caagcggata
      901 tcagatgatc gggaagatcg tttcaacgat gattgcattc ctctggaact tctttgcccg
      961 aaagtatctg ttgttcagta aaaaagaatc ataactggct gcaaaagcag ctttcacgaa
     1021 aaagcgccga cacagcctgc agattatttc gggatgctgt gcggcgtttt ttacaggcaa
     1081 aattgtagct ttgacaatgg aaaggagtga accattatga aaatgatatt tattggtgca
     1141 acacacgaag taactggtag ctgtacctat ctggaagtct gcgggaaacg cattctggtg
     1201 gactttggaa tggagcaggg gagagatacg tatgaaaatg tgaagattcc ctgtgcgccg
     1261 ggagaaattg actatgttct tttgacacac gcacacattg accactccgg cttgttgccg
     1321 ctgctctatg caggcggctt ttccggggaa atccacgcta ctgcggcaac gacttctctc
     1381 tgtgagatca tgctgcgtga cagtgcgcat atccaagaat ttgaggcaga atggagaaac
     1441 cgcaaggcga tgcgaaaagg cggcgaaccg tatgtgcctt tgtataccat gaaagaggcg
     1501 ttgggtgcat tagagctgtt tgtaccgcat ccgtatgatg aaaagtttac ccttgttcca
     1561 ggtattgagg ttcgcttcct ggatgctggt catcttcttg gttcctccag cattgagcgt
     1621 gtcgaaaaaa atcgtatttt ccggagacat cggaaatctc aatcagccat tgattcgtga
     1681 tccgcagccg ccgaaagatg ccgattatgt ggtgatggaa tccacttatg gaaaccggct
     1741 gcatgaaaaa ccgaaagggt atattccagg tctggtacag gtcctgcggg atacattttc
     1801 tcgtggcggc agtgtgatta ttccgtcttt tgcggtgggg cgtacacagg aattgctgta
     1861 ctatttcaag cagatcaaag acgagaacct gctcccggag tataaggatt ttccgattta
     1921 catggacagt ccgcttgcga ttgaggcaac agaagtattt atgcgaaata aggaaagttg
     1981 ttttgatgaa gaggcactga gttacgttcg gcgtggaatc aacccaatcg gtgtcagcgg
     2041 actgatccca gcagtgacc
//
LOCUS       DAWENO010000255         1936 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_17494_length_1936_cov_4.675173, whole genome shotgun sequence.
ACCESSION   DAWENO010000255 DAWENO010000000
VERSION     DAWENO010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17494_length_1936_cov_4.675173"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    886..895
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1721..1730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          501 a          487 c          487 g          441 t
ORIGIN      
        1 tccggcaggg actgcgttcc cacacagccc agagggttct gcgtggcgat aaccagatgc
       61 ggctggggca gggcataggt ctttccctcg accgtaacat ttccttcttc catcgcctcc
      121 agcagcgccg cctgcgtctt gctggaagtc cggttgatct cgtccgccag taagaggtgg
      181 cagaacaatg cgcccggctg aaacacaaat ttttccgtgc ttttctcata gacggaaaaa
      241 ccggtcagat cagagggcag cacatccggc gtaaactgca cccggcggca gctcagcccc
      301 agcgccttgg aaaacgccat ggcgagtgtc gtcttgccca cgcccggcat atcctccatc
      361 agcacgtgac cgccggcaag aaaggcgatc agcactttgg tcagaaccag atcttttccg
      421 attaccgctt tccgcacttc ccgaagaatc atcccaggga gtgcgtgcgg tgttgtagca
      481 gatggcatag tcattctcct tttctggagc ccgtttcaga acggcttgct ctcgcaaacc
      541 atgcttgaaa caggctctta cagacagcat ttcatgtgct gccgtttgga tggatacagc
      601 gcagcggcac aatagccggc atacgcttgg gaaatacagc atacagcagg gcgaaacgta
      661 cagaaaaatt ggagcgtttg cctgctgctt tacagttttt tcacgatata ccatttatta
      721 tatcattttt ttcggttctt ggcaagtgtg ctgactgtct tttccgcaaa atccccgcag
      781 ctttttttgt ttgctttcgg tgaaaacggc gaaaaaagca gaaataggga aaaaagcagc
      841 ggtatgatat tgacaaaacc gcaaaaaatg ttatactaga aaggtnnnnn nnnnncatga
      901 tatcaacccg aaacggatca cggtagatga atttgtcgct gttgtggaaa ttccaaaaaa
      961 cagcaaggtg aaatatgagc tgaacaagga gaccggtatt ctggaaatgg atcgtatctt
     1021 gtatacttcc acccactatc ctgcaaacta cggttttatt ccccgtacct atgcagatga
     1081 cggagatccg ctggatgtac tggtgctgtg ttcccagacg ctgcacccca tgacgctggt
     1141 gcgctgctat cccattggcg tgatccacat gatcgacaac ggacagcggg atgacaagat
     1201 cgttgcgatt ccctgcaacg acccgacctt caacagctat cataacattg atgatctgcc
     1261 acgtcacatt ttcgaggaga tcgtgcattt cttccaggtt tacaaagaac tggaacacaa
     1321 gacgactgcc gtagacgaag tagaggacgt atacgctgcc aaggagatca tcgctgcgga
     1381 catcgaccat tatatcgaaa agttctgcaa ggcataaatc gtaaaaagga cacgctgtac
     1441 gcaggcgaga accgccggcg tgtgcggctt ttctgcggtt aggcgtgcct aaaatagaaa
     1501 gaggtatttg agtatggtat cagcatcaga cattttgttc aatcacggaa ccagcgttgc
     1561 accgctggga ctgattgcaa tgaaaggcgc agaggaactg ggaaaaaagc tgaatgagta
     1621 cctggtaaac tgggcgcaca aaggcggcta tgacgtggac acattcctga tcgaatcaga
     1681 atgccctcgt ttctcttccg gtgacggcaa gggactgatc nnnnnnnnnn attatcattg
     1741 atgtgggaaa ctacaacgta aagtatcaga tgtacgatca gcagaacgcc atgtctccgg
     1801 acgaccacta ccaggatctg aagcgtatta tccaggcagc cagcggtaag gcacaccgca
     1861 tcaatgtaat catgccgatc ctgtacggcg gccgccagca tcgccggaat taccgggagt
     1921 ctctggactg tgcctg
//
LOCUS       DAWENO010000256         1910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_17734_length_1910_cov_8.506739, whole genome shotgun sequence.
ACCESSION   DAWENO010000256 DAWENO010000000
VERSION     DAWENO010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17734_length_1910_cov_8.506739"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          376 a          491 c          583 g          450 t
ORIGIN      
        1 cctgtgtctc gatgctgtat ttatagctgt caaattgttt caagcggggc agttctgctt
       61 cccagactgt gtctgagatc ttgtgcatgg gattggcggt gcggttccag ccgttgaact
      121 ctccgatcac ggagacgctc tttgcgtggg gcgcccagac acgaaagcgg tagaactgtc
      181 ctcttccacg ccggaacggg tgcgcaccga nnnnnnnnnn gaaaatcatt tgcggtggat
      241 ttctgtgatg attgcatagc ataccctcct tttgttttct attcatattt taacatataa
      301 aggatagtat tgcaagttat ttttttggta tccagtaaat ttcatagaac cgcccatatt
      361 tgaaccgcat atttagtcat gttgcataaa aacaaagacg tatcataacg gctttgtatt
      421 ctttttgtgg cgatatcgca caaagtacac ggcacatcac ctgcaattgt atttttggaa
      481 gaaaaatccg gaatctaaat gaattcagca aaaagcagct ttgatctggt aaaaattaga
      541 tgccgtaaaa ctgtgcgatg ctgtctcaga atgcggggag cagacgtgcg gcgcagacgg
      601 ttacatcatc ccggcatttt ccgccggcga agatctctgc cttttcgcag atctccatca
      661 cgatctgttc caggtcgctg gtggtttcca gcagctgccg gatcagcgga taggcgtcct
      721 cgctgacacc gtccgacagc atcagaatga tgtcatcggg gcagagcttt acctgccgta
      781 tcgctgtttc gccctccggt tccagtccga ggggaaacgt catggcgctg atgcgtgaga
      841 cctttccgcc gtggcggatc agtgtggcgg ctgccccgga tttcatcatg gtgagacacc
      901 cggcgtccag atcgaaccgg gcaacatcca gtgtggcaaa ggtctcctgg ggcgatttgg
      961 tcagcagcag cccgttcatc atgcggacag ccgctgtgcc ggagatgccg ccgctgataa
     1021 acttgcggaa cagctctgtg gtcatccggg actcgatggc agcatttttt ccggttccca
     1081 tgccgtcgga cagcaccaga taggggttgc cggcgctgtc agtgaagagg atggcagtgt
     1141 caccggagat ggtctcctgg ctggcgtgcc gggcggaggt gaagtgttcc agccggtatc
     1201 tgggacgctg gcagagccga tagcgcacag tctcgccgga gtagatgggg tccagttctt
     1261 ccagcgtcag attcagagat tcggacagga tgtgccggat ggtgggcaga cagccgtcca
     1321 gttcccggct ctgacagcac atctcgatca taagccggtc ggaaagggtg tggtacactg
     1381 cggcggagtc gcagggataa ccgtagtgga gcagcttgcg gcgcacggtc tcactgagtt
     1441 cgctgctgta gcggatgtgc agctgatcgc tggtggcggt gagcaggtcc tctgcggctg
     1501 ccagctgttc caggagaacc gtgcggctct cggcagtgcg tgccgccaga aagcgtgcct
     1561 tgcgccggtc ggcggcgtga cgggaaaaga gggtgcgcag gcgttctgtc cgaatgcagt
     1621 gttccagctc ctgcggaatt gcaggcggtg cggcactcag atgggtctcc attttccgaa
     1681 agccggcgag ggtgtcctcg tagttggtct cccagcagcg cagcttgttc cggcagctgc
     1741 cgcagaccgt ctcgcagact tcccgtgtgg cgtcgcctgt ggattcttcc tggggcagga
     1801 tcgccgagat ggcatccgta tcctcccgga cgctgcggat ggcgtctgcc atatactgca
     1861 gccgggaaga aaggggcagc gtgttatcag agcggtcggg caggtctgtg
//
LOCUS       DAWENO010000257         1899 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_17847_length_1899_cov_11.331887, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000257 DAWENO010000000
VERSION     DAWENO010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17847_length_1899_cov_11.331887"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1282..1291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          480 a          574 c          505 g          330 t
ORIGIN      
        1 ttcccaacgc tgtggtagac caatatctga aactggcgac cccgtcccag ctgaaagtgc
       61 tgctgtatct gctccgcaat aacgggcaga ccgtagacca cagcgagatt gccaaggcgc
      121 tggcgatctc ggaagagctg gtggaagagg cagccctgtt ctggtcgcag accgacctct
      181 tcgctgtcac agaaccggcg gcggaaagcg tcccggcaga gaccgcagcc gaaccggaac
      241 agaaaccggc gaaagcccca cgtaccaacc atgtttctgc ggtttccagc ggcaagagcg
      301 gcgatctgac accgctggaa atcgccagcg ccatcgaggc atccgacgat ctcaagaccc
      361 tgttccggat gtcggaacag cagctgggac gaccgctgcg gcacgtggag cagaaggcac
      421 tggtgtggat gcacgactac aacaacatcg gcagcgacat catcctcacc gtcctgctct
      481 actgcaaatc catcgacaaa tccagcgtca gctatgccga aagcatcatc acatcctggt
      541 ggaatgacgg catccagacg ctgcctcagg tcaccgacgc catcaacgag atggaacacc
      601 gccggagttt tacggggcat atccagaaag cgtttgagat gcaccgcaag cccacgccca
      661 agcagcagga atacatcgac ctgtggcaag accggaagat accgctggat ctcatcacct
      721 atgcctatga aaagactctg gagagcatcg acaagctttc gttcccctat ctggacagca
      781 ttctcacacg ctggatgaac gccggatacc gtacccggac ggatgtggac accaaagacc
      841 gcccggagac cggggacaaa aaagctgccg caaagaacgc cgtccccaag agcgacaacg
      901 ccgaggcgta tcaatccttt atccgcaatc tggaaccgta agaaccagtg tgttcgcacc
      961 agccggcact gaccaaagga gtacccaatg aacgaagaaa atctcgaaca cgccctgcgc
     1021 attatctcca cccggcgcaa cagcgccagg acgcagcagg aagagcgtat ccaggagatc
     1081 gagacacgca tcccggaagt ggtagagatc aaccgccagc tgttccgcac cagccggaac
     1141 ctgctggaaa tcatccggga cggcggcaat gtgagcgaac aggtggaatc cctgcggcgg
     1201 cagaatcagc aggggcaggc gatgatccgg acactgctgg aacagcacgg ctatccggca
     1261 gactatctgg aactgcacta cnnnnnnnnn ntgtgaaaaa tgccaggaca ccggcttttt
     1321 ccagggaaag acctgttctt gtctcaccga actggtggca aagctttcgg ctgagaaact
     1381 gaaccagagc gtccggttcg agcagtgcag ctttgagaac ttttccctgg actattataa
     1441 agggctgcac accgaaaacg gcggcagctg ctacaatgcc atggaacggg tgctgtactt
     1501 ctgccgggaa tacgcccggc actttacccc ggaatctccc agcatcctgc tgttcggaaa
     1561 gaccggtctg ggaaagacgc atctttccct tgccattgcc aaccaggtgc tgcgccgggg
     1621 ctacagcgtc ctctatgact cggtcattaa ctttctgcgc caggtggagc gggaacattt
     1681 tggcagggca agcggtgacg atgacaccct ggagctgctg ctgtcctgtg acctgctcat
     1741 cctggatgac ctggggacgg agttccactc ccagttctat cagtctacca tttataatat
     1801 catcaacacc cggatgaacc ggaatcgccc taccatcatc agcaccaacc tcaactacga
     1861 tgagatctcc caccagtacg atgagcgcat cacctcccg
//
LOCUS       DAWENO010000258         1842 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_18452_length_1842_cov_7.001679, whole genome shotgun sequence.
ACCESSION   DAWENO010000258 DAWENO010000000
VERSION     DAWENO010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18452_length_1842_cov_7.001679"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          503 c          441 g          471 t
ORIGIN      
        1 tgtcagcagg ataatgatga tgatcgccgc aatcaacacg aaatgtttcc tttcccgatt
       61 acagctgatc cacaacagat gcgcagcgtg cgcagagcgt cggatgtgct gcgttcttgc
      121 ctacgctgtc cagatacatc cagcagcgct cgcacttttc acctgcggca cgttctactg
      181 tataggttgt atcgccggat tctgccttga caacagaaac ctcagaaacg atgagaacct
      241 ttgccaggac atccgcagca tccgacagga ttgcatactg tgcatcgtca gcaacagaaa
      301 tggttacctt tgcctccaga gacttaccga tcagcttttc atttctcttt tcttccagaa
      361 ccttgttggc gtccatacga atctgataga tcagatccca ctttgccatg aagtctgcat
      421 cttctgtcag accgcttgcc ttgggcattt cgttcaggaa tacgctcttt gcatcatcgc
      481 ttgcagcgtg cggcagataa gaccaaatct cttctgcggt gaaggaaagg atcggtgcga
      541 tcagtcttgt cagtgcgctc agaatgcgat agatcgttgt ctgtgctgca cggcgttcaa
      601 cagaatcttc cttttcacag tacaggcgat ccttaatgat atccagatag aagttggaca
      661 gatctgtagt gcagtagttg tgaattgcat ggaatacaat gtggaagtca aatgcctcgt
      721 agccctcatg cgctttctgg attacgttgt ccagacgcat taatgcccag cgatccagtt
      781 ccatcagctg atcgtcagaa acgctgtcac gatccggatg gaaaccatcg ccgttgccta
      841 ggttacccag gatataacga gcggtgttac gaatcttctt atacgcatcg gaaagctgtc
      901 ccaggatcgc cttggaaatg cggatatccg agtgataatc cgaagatgcg acccacaggc
      961 gaagaatatc tgcgccgtac tgctttacga tttcttccgg catgatgccg ttgcccagag
     1021 acttggacat ctttctgccc tcgccgtcaa cgacccagcc gtgtgtgcaa actgccttat
     1081 acggagcgct gcccttgtat acaacagatg tcagcagaga ggactggaac cagccacgat
     1141 actggtcagc accctccaga tagagatcag ccggccactg gaggcagtcg tactgttcca
     1201 taacggcact gtgagataca ccgctgtcga accagacatc catgatgtca tattccttgg
     1261 tgaattcctt gcagccgcat tcgcactgta cttccggaga gaggaactcg gacagatcct
     1321 ttgtccacca tgcgtctgca ccttccttac ggaacagtgc ggcaatatca tggatagtgg
     1381 tatcatttac gatgggcttt ttgcagtcct tgcagtagat gatcgggatc ggcacacccc
     1441 aggtacgctg acgggaaatg caccagtcgc tgcggtcacg aaccatgccc ttgatgcgct
     1501 cttcgcccca tgccggaatc cacttgacat tttcgattgc tttgattgcc tcatccttaa
     1561 aaccgtttac agagcagaac cactgttcag ttgcacggta aatgattggc ttatggcaac
     1621 gccagcagtg cggatactgg tggacgatct tctcggttgc cagcattgcg ccgacttcgg
     1681 tcagctttgc agcgattgca cggtttgcct catcggtgtc catgccctcg aattcgcctg
     1741 cctctttggt ctgacgaccc ttatcgtcaa cacataccag gatgccgatg tcatcgtaat
     1801 tcttgcagac ctcaaagtcc tcgacaccat agcccggtgc ag
//
LOCUS       DAWENO010000259         1808 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_18839_length_1808_cov_10.242442, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000259 DAWENO010000000
VERSION     DAWENO010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18839_length_1808_cov_10.242442"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          438 a          454 c          554 g          362 t
ORIGIN      
        1 catgcagaaa ctggtagagg aacaggatat gacccgtctg ctgcacttcg aacagcagct
       61 gaccgttgtg ctggcggcaa tggaacatga cggcatcgcc atcgaccagc agggtgtccg
      121 ggattttggc gtatacctgt ccgaacagct ggaagaaatg cagcagatga tctatgacga
      181 ggcagggcat acctttaata ttggttcacc caaacagctg ggcgaggtgc tgttcggtga
      241 aatgcagctg ccccacggca aaaagaccaa gaccggctat tccaccaatg cggacattct
      301 ggaaaagctc cggggcgaat atgccatcgt ggactatgtg ctgaaatggc ggcagtacag
      361 caagctgcgt tctacctatg tagacgggct gctgaaaacg gtgggcgagg acggcagaat
      421 ccacactgta tacaaacaga cggagacccg gacgggacgt atctcctcca cagagccgaa
      481 cctgcagaat atcccggtac gcacagagct gggacgaaat atgcggaaat tctttgtggc
      541 acgggagggc tgtgtactgc tggatgcgga ctacagccag atcgaactgc ggctgctggc
      601 aaacctcagc ggcgacaagc agatgcagga ggcgttcctc agcggcgcag acattcacgc
      661 agccactgcg gcgcaggtat tcgatatgcc ccgtgaaatg gtaacaccgg aaatgcggag
      721 cgccgccaaa gcggtaaact tcggcatcct ctatggcatg ggtgcttttt ccctgtccca
      781 ggatatccat gttaccgtgc gccaggcgaa ccagtatatc cagaactata tggcacagtt
      841 ccccagcgtc ggcgcattta tggacaagac tgtggacacg gcgaagatca acggctatgt
      901 tactacctat ttcaagcggc ggcggcctgt gccggaactg ctggcgtcca ataaaatggt
      961 gcaggcggct ggaaagcgca tcgccatgaa cacgcccatt cagggaacag cggcagatat
     1021 catcaagctt gccatgatct atgtgtacca gcggctgaaa cgggatgtgc cggatgcaaa
     1081 gctgatcttg caggtacacg atgaactgat cgtagaagtg ccggaacagc acgctgtgct
     1141 tgccggaaag gtgctggggg aagaaatgca gaacgtgatt cgcaacaccc tttcgccgga
     1201 ggagcaggca aagttcccgg tgccgctgac ggcagatgta agtatggggg agagttggta
     1261 tgaagcaaaa ggctgagatc gttccggcat cggaacagga tctggcggcg ctggcggaac
     1321 tggcagagca gtgctttccc gatccgtggt cggaaaatgt tttccggcag accatgtgct
     1381 gctgctacaa ccgcatctgg tgcgccaaag tggatgggaa actctgcggc tatgcggcag
     1441 tatcccgtac cggcgacgcc gtaaatctgg acgatattgc cgtttcgccg gcgttccggc
     1501 ggcagggcat cggaagacag ctgctggact gggcgcacgg gcagttcccg gaaagcgaat
     1561 tctggctgga agtgcgggag tccaacgccg ccgccattgc cctgtatgaa tccgcaggct
     1621 atcggcaggt gggctttcgg aaacggtatt atcacgctcc agaggaaggt gctgtgctga
     1681 tgacacgaga ggtgaaaaaa acatgacgga actgctaatg caactgaaag cttttctgct
     1741 ttgggcattg tttcttgtac ctccgtttgt gctgactgca ctgaagttgt ggaatctttt
     1801 tgcggagt
//
LOCUS       DAWENO010000260         1759 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_19411_length_1759_cov_8.425469, whole genome shotgun sequence.
ACCESSION   DAWENO010000260 DAWENO010000000
VERSION     DAWENO010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19411_length_1759_cov_8.425469"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          378 a          482 c          440 g          459 t
ORIGIN      
        1 ttgccaacac tgagctgcag gtgaacgatg tgatcgtttc cgtccagggc gaggcggtgg
       61 atgattacga ttcgctgaac gctgcgattg ccggactgaa aggcggcgat accatggagg
      121 cacgctgccg ccggtatcag cccaacggca catttgaaga attcaccatc cagtgcaagc
      181 tgatggagga tacttctgga aactattaaa cagctgcaaa gcaaacagcc gccggcaggg
      241 ggaaaaaacc tgccggcggc tgttttgcaa tatttcagag ttgttatatt attccgcaga
      301 attcttttcg tttacttcgt caatgaagtt ctgggtctgg tcgtagatgg cgtttaccga
      361 ctcgttccac ggcacgcctc tggatgtggc acgaatgccg ttgacgctgc tgtccagaat
      421 atccgtgaca tcagaagaaa tgccgttgta atagtcaaat accgggtttt ccagcgccat
      481 ttcattcatc ttgtttttca tttcgatcat ttcatctgtc cactcatagt ccttgacgaa
      541 ctgttcgtca ctgatctcct gtgcgccgtc gctgatcttg ctgaaccgga agcagtccag
      601 gaacttgccc acgccctccg gattgtgacc gcctttgaca aagcagtatg cgttgacgct
      661 ggtgggaata tagtactcgt ccgccttgtc atacttgggg acaggaacaa agaatacatc
      721 ttcgccgaat gtcttggacc actgggattt ttcattgccc agcgcccaca gaccgcaggg
      781 atagaacagc agcttgccct cgccgatgta atagtcacgg gaagtccagc catagtcgcc
      841 tacgcccagc gcaatgtaat cggactgcca cagcccgtac atccagttct gatagtcctc
      901 gatcgcctcg ttccgcaggt tgttcaccag cttgccgttt tccagaccga tgtacggaac
      961 accggtggat gcagacagac cggactcgaa gtaccagctg tcgatgccgt aatgctcatc
     1021 ggaggtgtcc acaaattcag acagcatatc ctcgaaagcg tcccagtccc atttgccctt
     1081 ttcgaacagc tctgccgggt catccagacc gttttcgtcc atggtcttgc ggttgtagat
     1141 aatggcacag ttgtcgccgg tcacttctac gatcgccata tagtgaccgc cgttccacat
     1201 catagcgtcg ttggcatcct ttacgtctgc ccacagctct gaatccaggt cgatgtaatt
     1261 gtcatagggg acgaacattc cacggacagc gcccttcggg aaagcgtccc agttgccgcc
     1321 gtagaaaaag tctacgccct cgtcgctgtt gatggcgttc gccaggttgt catatcttga
     1381 accataggtg gtctgatacc attcgatctg tccgccgtac cgttcctgga acactgccag
     1441 ttcgataggc gtggtctttc cggtatcgtc cgagttgata tcccagtcgg acatccactt
     1501 gatggttttg ttttccagtt ctccggtcag acgctcgtct gactttgcca gttctgccag
     1561 ctgttctctg gtgttggcgg acatatcctt tttcggggtg gtgtccttgg tgtcagaacc
     1621 gcaggcagcc agcgggacgg tcagcaccat tgccagcaga cagtgcaggg caattcttgc
     1681 gctcttcttc ataaacattc tcctgtctga ttcgaagtaa tatactttat tataccatga
     1741 aacactgctc cggcgcaat
//
LOCUS       DAWENO010000261         1730 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_19764_length_1730_cov_8.017313, whole genome shotgun sequence.
ACCESSION   DAWENO010000261 DAWENO010000000
VERSION     DAWENO010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19764_length_1730_cov_8.017313"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          386 a          450 c          513 g          381 t
ORIGIN      
        1 ggattaagga ggatgtatca tgcgcaagat ctatgaccct atggaatttc agccgggaat
       61 gcagcagatc gactcggaca tttttgaccg tgtgattgcc gcaagaaagg cataccagtc
      121 ggaacaatac acggctcggc aggtaaaggc ggcgctccag aaggaagaat tatcttttga
      181 ggacttcgct gccctgctgt ccccggcggc acagccgttt ctggaacaga tggctcgtcg
      241 tgcaaagacg gaacgggaaa agcacttcgg caacaatgtg ctgttattta cgccgctgta
      301 tatttccaac tactgcgaga accagtgcgt ttactgcggt ttcaactgca aaaatgccat
      361 cactcgctgc aagctgaacg cagaggaaat tgaggcggaa atgcagaaca tcgccaaaac
      421 cggaatgcgt gaggtgctgc tgctgacggg cgaaagccgc tcggtttccg gtccggaata
      481 tattgccgag gcggttcgca ttgcgaaaaa gtatttctcc accattggtc tggaagtcta
      541 ccccatgaac gccgatgagt acgccatgct ccgggaatgc ggtgcggatt tcgtcaccgt
      601 ctttcaagaa acctacgacc tggagcgcta cggcgaaatg cacgttgctg gtgcaaagcg
      661 gtgtttcccc tatcgtgtct gcgcacagga acgggcgctg atgggcggta tgcgtggcgt
      721 tggatttggc gtgctgctgg ggctggcaga tttccgaaag gatatgttcg ccgccggtat
      781 ccatgcggcg ttattgcaga agaagtaccc ccagggtgaa attgccctgt cgttcccccg
      841 gctgcgtcct tataagaacc acgaggagac caaccccaac gatgttcacg agacccagct
      901 gctccagatg atgctcgcct gccgcatctt cctgccgttt gcaagcttta ccatttccac
      961 acgggaacgt gccggattcc gggatcacgt tctgccgttg gcggcaacca agatctctgc
     1021 cggcgtcagc gtgggcatcg gcggtcacaa tgaggaacac aagcaaaagg gcgatgcgca
     1081 gttcgaaatc tctgacccca gaagtttgca ggagattcat gaggcgattc tggcacaggg
     1141 tatgcagccg gtttataccg actacatcaa tacgaatatc ttatgatgat ctgtgtgaca
     1201 aaccgcaagc tttgccgggg tgacttcctg gaacgcattc ggcagcttgc cgccggcggt
     1261 gcggatgtga ttttgctgcg ggaaaaggat ctttctccgg cggcttacac agaactggcg
     1321 gaaaagtgcc ttgccatttg ccgggaatat agcacagcgc tctggctgaa cagcttcacg
     1381 ggaacagcgg cggcgctgca attgcctgta cagctgccgc tttcggtgct gcaagagttg
     1441 acagatgcgg ataaggaaaa actgcctgcg ttcggggcat cggttcacag tctggcagat
     1501 gcgcagctgg cagaaaagct gtgcgcagac tggctgatcg ccgggcatat ttttcccaca
     1561 tcctgcaagc cggatctgcc gccgagaggg ctggattttc tgcgggaaat ttgcagtcac
     1621 gtttccgttc cggtgtatgc catcggcggc atcacacctc agcggcaggc agaggtgcgg
     1681 caggctgggg catccggctg ttgtgtcatg tcgcagatga tgcagtgtcc
//
LOCUS       DAWENO010000262         1729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_19778_length_1729_cov_10.556750, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000262 DAWENO010000000
VERSION     DAWENO010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19778_length_1729_cov_10.556750"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1481..1508
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          297 a          459 c          519 g          426 t
ORIGIN      
        1 ctgtgcgtcc tcttctgtat agctgtccgt gccgtccagc cgggtcagcg tgccgtcgga
       61 attcgcaaag cacagaaaat ggtcgatggt gactacgccg ctctttttat ccggaaacgg
      121 cggttcgccg tggtcgtcct ggcgcatggg catagactgg gtcagctgcg ccagcggtgc
      181 ggcatagaac accccctgct ttgcggcagc atagttctct tcgttttccc agcggtgatc
      241 cggttcgtcc ggtccccacc agggcggcgg atcgggatcc ggctgcgtcg tctgctgggg
      301 tggttttgtc tgcggcggcg cagtggtcac cgcaggcttt gtcgcccgtg ggctggtggt
      361 tcttgccgca gtggttgccc gtgtctgcgg tgcggcagtg gttcgtggcg ctgcggtagt
      421 ctgtaccgga gactgcaccg ccggcacctg ggtctgcacc ggatttttgg gctgttccgc
      481 aggcgcagct tttgccgttg tctctgcggc agcggtctcc gtggctgtgg tttccgtggt
      541 agtctctgca gtcgtttccg cagcggaagt gccggtctcc gtggcagtat ccgtggactg
      601 ccggggctgt tttccgtccg gcggcatatc atccggcagt tcgggaacgg tggtctcact
      661 aatggcaatc tgttccagca ggtttcgggt ctggttatag ctcatcgcct cggtcaccac
      721 attgagggca atcagcggca ccagcagcac tgcggtgagc atcgccatcg tgatacagat
      781 aaactgccgg cgcacccggc ggatcatgct tttttctcca ggaaatagcc gatgccacgg
      841 gcggtgcgga tctgcacagt ggagtggatg gcggtcagct ttttccgcag gaaggagata
      901 taaacctcga tattgttgta ttccacgtcg ctttcatagc cccagatctt ttcgataaag
      961 cgttctttgg gcaggatctg tcccggattg gagaggagca tttccatgat ctgatattcc
     1021 ttcagactga gtttcatccg gctgtcgccc tggatgaggt cataggtctg gcggttcagc
     1081 tggagttcct caaagtgcag gcagtccact tccacctcgc ctttccgccg ggtcagcgcc
     1141 cggatacggg caaggagttc gccggtggaa aaaggctttg tgaggtaatc gtctgcgccg
     1201 cagtccagcc cgtggatctt gtcttccacc tcagactttg cggtcagcag cataatgggc
     1261 gtggcgtttt tttcctgccg caggatacgg acaatctcgg tgccgctgcg aaccgggagc
     1321 atgatatcca ggaggataca gtcatagata ccggtacggg cgtattcgag accgtcatcg
     1381 ccgtcaaaga cggtgtccac gttgtacttg ttgcgtttga ggatttctgc cagggtatcc
     1441 gccagctgga cttcatcctc gaccacgagt atgttcattg nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnntt tcgaaagcgt aggtttccag tatttattgt aacggggaaa gcttaatgca
     1561 agcttaaaca ttgcggcggg ctgaaaatcg aaccgattgt ttctttgaac aggacaactc
     1621 ctctgccctg tcgggcatct cctctaaaag aggagacatt tcgcaaagcc tcctttacag
     1681 aagaggtgac acgaagtgcc ggaggagtcc cgggcgaaca atgttcgcc
//
LOCUS       DAWENO010000263         1700 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_20162_length_1700_cov_8.331307, whole genome shotgun sequence.
ACCESSION   DAWENO010000263 DAWENO010000000
VERSION     DAWENO010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20162_length_1700_cov_8.331307"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          448 a          437 c          462 g          353 t
ORIGIN      
        1 atcaggcaat ctcagagaaa tatcatattc cgctggatgt tccggtaaaa gaactgacga
       61 aagagcagct ggatctgttc ctgtacggca caaaggaaga accgctgttt ctgcaccgta
      121 tcacagccgg cggcagaagt gagggattcc gtgcgccgtt cgaaggcgtg atcccgaatc
      181 tgaaacggcg ctttgcagaa ggcggcgagt gggcaaaaga cgagatcagc gcagtaatga
      241 gcgatgtagc gtgtccggtc tgccatggaa aacgtctgaa gccagaagtc ctggcagtta
      301 ccgtaggcgg aaaaaacatc atcgagctga cagaactgtc cgtcacaaag gctctcgcat
      361 tcttcgacga actggaactg tctgaacggg aacagttcat tgccgcaaga atcgtcaagg
      421 agatcaagga gcgtctggga tttctcaaca atgttggtct ggaatatctg acactgtcac
      481 gaaatgccgg cacgctttcc ggcggtgaaa gccagcgtat tcgtctggcg acgcagatcg
      541 gctcctatct gatgggcgtt ctctatatct tggacgaacc gagtatcggt ctgcatcaga
      601 gagacaacga caagctgatc gccaccctaa agcggctgcg tgacctgggc aatacgctca
      661 ttgttgtgga acatgatgat gatacgatcc ttgcagcaga ctatgttgtg gacattggtc
      721 cgggagcagg cattcacggc ggaaacgttg tctattccgg agatgtcaaa ggcttgcttg
      781 cctgcaagaa atccattacc gggcagtatc tcagcggtga gcggaaaatt ccgctgccgc
      841 tggaacgccg caccggaaac ggaaattatc tgaccattca cggagcaaga gaacacaatc
      901 tgaaaaatat tgatgtcacc attccgctgg gtgttttcac ctgtgtcaca ggcgtttccg
      961 gttccggcaa gtcctcgctg gtcaatgaga tcctgtacaa atacctggca gccaagctga
     1021 accgtgccag aacacgccac ggggattttg acggcatgga cgggacggaa ttcctggata
     1081 aggtgatcga catcgaccag tctcctatcg gcagaacacc ccgttccaac ccggcaacat
     1141 ataccggcgt tttcacagac atccgtgacc ttttcgcaaa gacaaacgat gcaaaggcaa
     1201 agggatacac tgccggacgg ttctccttta atgtgaaagg cggcagatgt gaggcgtgcc
     1261 agggtgacgg catcctgcgc attgaaatgc acttcctgcc ggacgtttac gttccctgtg
     1321 aagtctgcaa gggaaaacgc tataaccgtg aaacgcttgc agtggaatac aagggaaaat
     1381 ccatctatga cgttctggag atgacggtag acgaaggcgt cgttttcttt gaaaaccacc
     1441 cgaaaattgc ccgaaagctg aaaaccctcc aggaggttgg tctgggatat ctgaaaatcg
     1501 gacagccagc cacgactctt tccggcggcg aggcacagcg tgtcaagctg gcgacagagc
     1561 tttccaagcg tcctaccggg aaaaccatct atatcctgga cgaaccgacc acaggtctgc
     1621 acaccgcaga cgtccaccgt ctcatagagg ttttgcagcg tctgaccgac accggcaaca
     1681 ccgtagtagt catcgaacac
//
LOCUS       DAWENO010000264         1673 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_20504_length_1673_cov_11.336218, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000264 DAWENO010000000
VERSION     DAWENO010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20504_length_1673_cov_11.336218"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          466 a          354 c          456 g          397 t
ORIGIN      
        1 ctgcaaacgc aaaaaaacgg cacgtgatcg tagaacagag actgaaacaa atggcggagg
       61 atgcctgcca ttttgaaatc aaccgtacgg aatatcgtga taagaaaatc ggcgtaatca
      121 ccagcggtat cccctatcag tacgttcgtg aggcacttcc aaatgcctct gttctgaaat
      181 tgggtctggt acatcccctg ccgagaaagc tgatcgaaga atttgcggca caggtggaga
      241 cactttacat tgtagaagaa ctagaaccgg taatcgaaga gcaggtgcgt tcctggggaa
      301 tcccggttat cggcaaggaa attttcacgg tacagggaga atacagtgca aatatgctga
      361 gaaaggcaat tctgcatgaa gaggttgctc ctgttgtacc ggcacaggcg ccgaaccgtc
      421 cgccgattct ctgtccggga tgtccgcacc gcagtgtctt ttctgtgatg aagaaactga
      481 aactacacgg tgcaggtgat atcggatgct atactctggg tgctgtaccg ccgctgggtg
      541 ttatggattt caacgtatgt atgggtgcaa gcatttctac tctgcacggc attgaaaagg
      601 caaagggaaa agactatgtg aagaactggg ttgcactgat tggtgactct accttcctgc
      661 ataccggtgt caattctctg atgaacatgg tatacaacaa agcgactggt acagtgatta
      721 tcctggacaa ctctactacg ggtatgaccg gtcaccagga tcatgctgca acaggcaaaa
      781 cgctgcaggg cgaacttacc tatgcaatca atatctatta tttgtgtaag gcaatgggca
      841 ttgaaaatgt ctacgagatt gatgcgtttg atattgaggg actggaaaaa ctgctgaagc
      901 aggaaaccca gcgagatgcc ttgtctgtta tcattaccaa gtccccctgc gctcttctga
      961 aggaatttgt cccgaaggga aagtgcattc tgcatgaaga tgcctgcaag aagtgcggac
     1021 agtgcctggt tccgggatgc ccggctatgg taaagcagga aaacggaatc ccgaaaattg
     1081 atgcggcgat gtgcaatggc tgcgaactct gcgcaaaacg atgcaaattc cacgcaattg
     1141 aactcattcc tcgggaaaaa ggaggtaagg cataatgcag actatcaata ttatgatcgt
     1201 tggcgtcggc ggacaaggta cacttctgac tagccgtgtg ctgggaaaaa ttgcgttgac
     1261 cggcggctat gatgtaaaac tgtccgaagt gcatggtatg gcacagcggg gcggaagtgt
     1321 tgttacatat gtgcgttatg gcgattctgt tgcagaaccg atcgtagaag aaggacaggc
     1381 agatattctg attgctttcg aacgcctgga ggcgctgcgg tatgcacact tcctgaaacc
     1441 gaccggggca atcattgtga atgacacacg gattgatcct atgtctgtcg tgattggtgc
     1501 agcagaatat ccagaacaca ttctggagaa tctgcaaaag aaatataagg tgctggctgt
     1561 ggatgctacc gcagaggcgt taaagctggg aaacccgaga gttttcaaca cgattattct
     1621 cggcgttgca gcacagcata tggatttctc taaggaagaa tggttacagg tga
//
LOCUS       DAWENO010000265         1673 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_20511_length_1673_cov_5.539555, whole genome shotgun sequence.
ACCESSION   DAWENO010000265 DAWENO010000000
VERSION     DAWENO010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20511_length_1673_cov_5.539555"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          365 a          457 c          379 g          472 t
ORIGIN      
        1 gtccggaaag gagaattttc aatgctgtga atattccgct gaaaaccgag ttccgcagaa
       61 tatgatgtca taccgccacg tcttgtcccc agatgagagt gatagtccgt cccataaaat
      121 acgtcaagaa tgcagtcgtt tgctgaaact gcaccaaaaa agccgcccat ggattattca
      181 ccaaagatgc gcttcataac ttcctgataa gcctcttcgg taccgcccat atctctgcgg
      241 aaacggtcct tgtccagctt ttcgttggtc ttgacatccc acagacggca ggtatccgga
      301 gagatctcgt ctgccagaat gatggtaccg tcagccagac gaccgaactc gatcttgaag
      361 tcgatcagac ggatgccgat ggacaggaag aacttcttca ggaattcatt gaccttgcgg
      421 gtatagtcct tgatggtgtc gatctcttcc tgtgttgcca gacccagtgc cagagcatag
      481 tcatcattga tgaacgggtc gttcagttcg tcgcacttgt agctgaattc caggatcggg
      541 cagagcagat ccttgccttc ttctacgccc atgcgcttgg aaaagctgcc ggctgcaaca
      601 ttgcggacga taacttccag cggtacgatg gaaaccttct tgacagcagt ttcacggtcg
      661 ctcagttctt ctaccaggtg agtcggaacg cctgcttctt ccagctgttt catgatgtag
      721 ttggacatac ggttgttgat aacgcccttg ccaacaatcg tacccttctt ttcgccgttg
      781 aatgcagttg cgtcatcctt ataatctacg ataacaactt ccggattctc ggttgcgtat
      841 actttctttg ccttgccctc atagagctgt tctgttttct tccaatcagc cattttctgt
      901 tcctccaaaa ataaaatgtt ttgattccag agtctgtcag actcttattt taactcagca
      961 gttactgtat tctgccgaaa tttttgcacc ttttgccttt acgccctgct ccatatctgc
     1021 cttcatctgt tccagctttg catcaatttc cggatcagac agtgccagca tctgtgcagc
     1081 caggatcgct gcgtttgctg cgccatcgat ggcaactgtt gccaccggga ttcccttcgg
     1141 catctgaacg gttgccagca gtgcatccag tccgtccaat gtggacgact ttaccgggat
     1201 gccgacaacc ggcagtgtgg tgtgcgctgc cagaacgccg cccaggtgcg cagcctttcc
     1261 tgctgcggaa aggatcacgc caaagccgtt tttcttggca ttggacgcaa attctgctgc
     1321 cagcgccggt gtccggtgtg cagacataac gtgtacttca tagggaatgc cgaacttgtc
     1381 cagttcttcc agcgcgccct tgacaaccgg cagatcactg tcgctgccca tgataaccgc
     1441 tacttttttt gacatatgta ttcctccaga ttcagagttg cacatccgct caagacttgc
     1501 cttaccgcaa gttcccggag cgtgtgcggc tgatgcaggg tacagtcccc tgcaaattta
     1561 tgaaaacaga acacaaaccg gactcagccc agttttgcct ctgttatcaa ccatatttct
     1621 tttccgcttt tctgcatttt tacagtcagc gttctggtct gcgtattgtt atc
//
LOCUS       DAWENO010000266         1630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_21150_length_1630_cov_12.760635, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000266 DAWENO010000000
VERSION     DAWENO010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21150_length_1630_cov_12.760635"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          349 a          436 c          455 g          390 t
ORIGIN      
        1 ctggtcggtc tctgccgccg tcagcaggga tgccggcgtg agccgggaca ggtcgccgtt
       61 cagcgccgca aacagtgccg gggtgttctc ttcggagaag accgggcgga ttttgtctgc
      121 cacagcctct gcggaaagct ctgcccggac gctgcccctg ggatagccct ccatggctct
      181 gccgtccgga tcgacgcaca gacgctcgat aaaggcatct ccggcagctg ccgccagcag
      241 gggcgtgttc ccgttgatgg ggatggcgtg atagctgccg ctgtcccagt acaggaatac
      301 ctggttctcc gccttggcgt ggatctcgtt gtagtgcagt cctgccatgg caaattccat
      361 gacggcgctg ttgatataga tcggcaggtg caggctgtcc tccagcgcct tttgcagcgc
      421 cggaaagacc ggacaggtct ccgtgccttc ctccaacccc atcatttcat aggcagaggg
      481 catgatgccc acgccgatac ccagaagatt ctcccgggag agacagctgt tctggagcat
      541 tttttccagg atgttgtcca gcagcgacac cagcttttcc ggtgtcatgt caccctcgtg
      601 ggcgatcagc tgtttatcca gcgctgccgc atttaaggta gtcagaccga tggagacttc
      661 cttttcgtcc acgtagatgc ccactgcaaa tttatatgtt tcattgatgt ccagcaggat
      721 ctttcgtctg cccttgcgga cctcgccgct ggggttcacc ggctcttcgc cgacctcttc
      781 caggatgtgc tggtcgatca tttcgttagt gatgatcgtc actgccgcac gggtgatccc
      841 cagaattgcc gaaatgtcca cacgggagat gggaccctgt tcccggacaa ccttcaggat
      901 ctgcatccgg ttgcgccgtt tcagatccag tttactgata ccgcagtcat tcatgttttt
      961 cttcctcttt ttgaaatcag attgctgagc gtgtttcgca ggattcccgt tcgggatgct
     1021 gtgcaatctg cacacggcta aatacgaaac ggacgcttat ttatctattt taccatatgc
     1081 aaaaccggtt tgcaaggtgt ctgcggattt tacttaaaaa tttacaaaaa tgacacggaa
     1141 cggaacaggt tatgccacaa ccgaaaaaag atagaaaaat gcgtgagaaa attcggacac
     1201 cggcagccgg tgcggttgga actgcggaga aattggaaga gcgttgattt tgtttgttca
     1261 tatttttgct taaaaaggtg tgccggactt gaaaaagtaa gttgcaggct tgatggaaag
     1321 tctgattccc ctgtcccgaa aggacagggg gataacccct cagtcaagcc tacggcttgc
     1381 cagctcccct gttaggggag ccaaaagaaa agtctcctct tatagaggtg attccccacg
     1441 gggtggggaa atgtcaccgt aggtgacaaa ggggaccgct cagtacgaga tgcccgacag
     1501 ggcagaggag tcgatatgtc ccccttgaaa aggcacccga agggcgtgcc ttgtgctggg
     1561 atgtcagcgt aagctgacag ggggataacc cctcagtcaa gcctacggct tgccagctcc
     1621 cctgttaggg
//
LOCUS       DAWENO010000267         1612 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_21420_length_1612_cov_7.319204, whole genome shotgun sequence.
ACCESSION   DAWENO010000267 DAWENO010000000
VERSION     DAWENO010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21420_length_1612_cov_7.319204"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          480 c          407 g          387 t
ORIGIN      
        1 gccgaacgct tcccagttgt tagtaaatgt aatcagcagc tttacgcctt cctgctccgc
       61 cttgtacagt gcatagtcca gcttttgcag accgtcctcg ccctcgttga cgaccggctt
      121 gttcaggtca gcgtcgtaat actggtagta cacgccttcc ttttcaccag aaccgtctac
      181 gctgtcggta aacacggtat agcccttatc ggaaacctta tcggtcttga cgccggcatc
      241 caggttgccc caggtacgga ctactttcat gcccattgcc gcagcatctt ccagcgctgc
      301 gtctacggtc gccttcggtt tgaagttgat gtagtagttg tttgtgccgg catagtaaaa
      361 tgtcgaacca tccagcatga actgtgttcc gttggtggtg acaaatccgc tgcggctgtc
      421 tgccgcctgc acggtcactg cgccggaaaa cggcattgca attgcactgc acccaagcac
      481 cagactgctt gcggctgcga tccatttcct cttcatttgt tcctcctcat aacattgtgt
      541 ctgcctccgg tgtttgttcc gcagggacag acgggacaat tacaacctta cctcttactg
      601 ctgacgcata aacgccagat tcttgtcgat cagctcacag cgctgcttga cacagtctgc
      661 actgcggagc ggatcgccgg gcgtatgaaa cgcataatcc aaaagccgct tgatgtcagt
      721 tgtggcaacg tgcagccggg tgtcggaaga tgcgtaatag atataaaccg taccatccgg
      781 gcggacgatc gcaccgttgg tgaaaaccac attggaaaca tcgcccacac gttcttctcc
      841 cagcggcgcc aggaagtagc cggagggttc tgcgatcacc ttccacggct cgtgcaggtc
      901 ggtgacaaac acatacagca catagcgaag tcctgccgct gtattccgca cgccgtgcgc
      961 caggtgcagc cagccctttt ccgtttcgat cgggacagcg ccggcaccgt ttttggattc
     1021 ggtgatggtg tgatacttcc gcaggctggt catgcgttct tcttcgatca ctgcgtttgt
     1081 gatatcgtct gccagcccaa agccgatgcc gccgccggaa ccggtctcaa tgaagccatc
     1141 cataggtctg gtgtaaaacg catacttgcc gtccacgaac ttcggcagca ggcacacatt
     1201 tcgctgctgg ggactctgct tggtcaccag attcggcagg cgttcccagg tgttcagatc
     1261 cctggtacgg acaattccgg cagccgcaac tgccgcagac agatcagacg gatctttggc
     1321 gctggattct gaacaaaata cgccgtagat ccagccgtcc tcgtgctgcg tcagacgcat
     1381 atcatagacg tttgtctcat ccggatacag atccggcagc acaatgggct tttcccgaaa
     1441 gcggaagttg tcgatgccgt tttcgctttc cgccacggca aaaaagcttt tccggtcatt
     1501 gccctctacc cgaaccacca gacaatactt gccgtccaga tagatcgctc cggcattgaa
     1561 tacggcattg acacccagac gctccaggaa aaacggattc gtctccgggt tc
//
LOCUS       DAWENO010000268         1612 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_21421_length_1612_cov_6.977521, whole genome shotgun sequence.
ACCESSION   DAWENO010000268 DAWENO010000000
VERSION     DAWENO010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21421_length_1612_cov_6.977521"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    535..544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          355 a          476 c          363 g          408 t
ORIGIN      
        1 ctctaccacg cagacgctgt tccagttccg acagagacgg cggcaggatg aacacaaaga
       61 ctgcatccgg acgagccgca cggactttca ttgcgccctc gacctcgatc tccaggatga
      121 cgttctgtcc tttggcaagg tgttcctcca ctggctgttt cggcgagccg taataatgct
      181 cacagtagcc ggcatattcc agaaagccgt cctctgcgat cagcttttca aacgcctcgt
      241 tggtcatgaa acggtaattc acgccgtcca cttcgccggg acggggcgca cgagtggttg
      301 ccgaaacaga gtagtacaga ttcgggtctt tcagcacctc cgccagaatc gttcccttgc
      361 cgcagccaga cggaccggag acgacaaaca atgttccttt tttcatgctg cacacttcct
      421 ttattcgatg ttctggatct gttcccggat cttttcgatt tccgatttca tatccactac
      481 cagcttggtg atttccagag actgtgactt cgaaccggtg gtgttgacct cccgnnnnnn
      541 nnnnatcttt ctgcctgtgg gttccgtgct ttccaggaat ccccggaact gggcaatatg
      601 gctatgcagc cgcaccagtt cttcatccac ggcgattttt tctgccacta cagcagtttc
      661 cgtcaccaga cgagactcct ccactgtgcg gtcacccagc agctccgtga ttttcttgta
      721 cagcttatca taatactgct gtactgtttc cggaacaagc tgttccacac gattggtcat
      781 ggactccaga ttttccagat gagatgccac atccgcagcc aggcgttcgc cctctgtttg
      841 acgcatggtc acaaactgct gcagcgcctc ggtcacgacc ggctgcacct ccgaccagat
      901 cacatcagca tcctccgtaa cctgctgcac ctggaacgca tccgggatgc ggagcagtgt
      961 ggaaagcggc agtgcatctg tcgtaccgcc ggactcgctt cccgcagttc ccaacacgat
     1021 ctgatttttt ggaagaccgg attcccggag cagttcgttt tccgctgcaa cagcacgcag
     1081 gtatgccatc accacatcgt ggttgatctc caccgtcgta tccttgctgc cgatggactg
     1141 catggtgagc gcaacctcaa cctttccacg agagactgcc tgctgcacct gttccttgat
     1201 ctttccctcc agatagctga aactgcgggg cagacgcaca ctggtttcca gataacggtg
     1261 attcaccgaa tgcacttccg cagtgatctc ataacgttcc gtccgagccg tagcacgccc
     1321 gtaacccgtc atacttttca cttctgtcac actccttatg aaactgcacg tatcccatgc
     1381 aataatagtc catagtatct tattatagca ttttcacgaa aaaaaatcaa gtcaaatgca
     1441 gacacttttt tccatacaaa aagcgccgca gcatatttca gctgcggcgc ttttctattt
     1501 acggaaatcg tttacaaaaa cgatctcaat tatgcgttca gatcagaaga ttccggcaga
     1561 gccttgatga tgtgtaccag gaacttcagc agagcggtag catcgttacc gt
//
LOCUS       DAWENO010000269         1568 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_22083_length_1568_cov_8.902181, whole genome shotgun sequence.
ACCESSION   DAWENO010000269 DAWENO010000000
VERSION     DAWENO010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22083_length_1568_cov_8.902181"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          483 c          392 g          355 t
ORIGIN      
        1 cctggaatgc ctcaaagaca gttacctgat agcccaggcg tgccagatct ccggcacagg
       61 tcagaccagc cgggccgccg cctacaatgg caacacgctt gccgttcggc gcagcctttt
      121 cctgcttgcc ggtatagcca tgctcacggg catagtctgc cacaaaacgc tccagacggc
      181 cgatggcaac cggctcgccc ttcttgccac ggacgcactt gccctcgcac tggttctcct
      241 ggggacatac acggccgcag acagccggca ggctgttggt ggtgcggatg atgtcatacg
      301 cctggaggaa gtctccggca gccacagcag cgatgaaatc cgggatcgga acgcctaccg
      361 gacatccgct gacacagggc tttgccttac agtgcagaca gcgggtcgcc tcctcctgtg
      421 cctgttccgg ggtatagccc attgcaactt cttcaaaaca ctttgcacgt tccttgggat
      481 cacgctccgg cataggaacc tttgtcggtg tcatattgaa ttttcctgcc atggtcactg
      541 tacctccttc cggaacagat tgcattcctg ctcctgccgc ttgtacgtgc cgttgcggcg
      601 catcaattcg tcaaaatcta cctgatgacc gtcaaagtcc ggaccgtcta cgcaggcata
      661 cttggtcttg ccgccgacgg tcacacgaca tccgccgcac attccggttc cgtccaccat
      721 gatcgggttc agcgaaacga tggtctttac ctggaacggc tcggtcgtct tacagacaaa
      781 cttcatcatg ggaaccggac cgatagcaat gacgacgtca tacttcttgc cctgttccag
      841 ctgctgctgc agcacattgg tcacaaagcc ctttgtgccg taggaaccgt catcggtgca
      901 gagatacagg ttatcgctgc acgcacggaa ttcatcttcc agaatgacaa tatccttgtt
      961 gcggaaacca acgatggtat ccacttccag acccaggctg tgcaggtgct ttgcctgggg
     1021 atatgcgatg gcacatccta cgccgccgcc gatcacgcag accgactttg tgccttccgg
     1081 cagttctgtg ggaacgccca gcggtccgac cagatccagg atggtgtcac ctgcctccag
     1141 ctgtgaaaac tggcgtgtgg tctcaccggc gatctggaag atcagcgtaa ttgtgccgcc
     1201 ttcacggtca taatctgcta cggtcaacgg tacacgctca ccgaattcgt ccagccggaa
     1261 aatgataaac tgtcccggca gaaccttctt tgcgatcagc ggtgcaacaa tgcgtaccat
     1321 cacaactgca tcgttgagct ttttcttttc tacgatttga aacaattctt tcacctcatg
     1381 caatctcatt gactgtgccg gatgccggag cctgtcccgt atccggaaac atccccaaat
     1441 tctgacaagc cgaatgaaac acgccgacag accgccggct tgtttgaaat acatatgtgc
     1501 agcgctgccg tataaacgga cagcagtcga atcttagtcc gcagaatcgt cctctgccgg
     1561 cacgtcaa
//
LOCUS       DAWENO010000270         1546 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_22439_length_1546_cov_14.537223, whole genome shotgun
            sequence.
ACCESSION   DAWENO010000270 DAWENO010000000
VERSION     DAWENO010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22439_length_1546_cov_14.537223"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          444 c          422 g          384 t
ORIGIN      
        1 gtcagcttac gctgacatcc cagcacaagg cacaccctac gggcgccttt tcaagaggga
       61 caatgaatca cctcataaaa ggaactgacg caccctcaaa acttctcata ctcatgcttt
      121 ttcgggcggc tcatcaggtt gtccagcgcc tgtttcgggt ctgcgccgtc aaacaggatg
      181 tgatacagct gctccgtaat gggcatggaa acgccgtgtt tctgcgccag cgcccaggct
      241 gcgtgacagc agtaatagcc ctcgactgtt cccacctgct tgaccgcatc ttcgggagaa
      301 gttccctcgc cgatgaggat acctgcccgg cggttgcggc tgtgtatact acaacaggtc
      361 acgatcagat cgccgatgcc ggacagaccg ccgaatgtct ccggctttcc gcccattttt
      421 acgcccaggt tggtgatctc atggatgcca cgggtcatca gcgccgcctt ggtgttgtcg
      481 ccgcagtgca gcccgtcgca gatgcctgct gcaagagcga tgatattttt cagtgcgccg
      541 ccaatctcac atcccaccgg gtcggcgttg atgtagatgc gcagggtgtg gctggagaaa
      601 acctcctgga tctgcaccgc cgccgcctcg tttttggacg cagccactac cgtggtcggc
      661 acgccacgtc ccacctcttc cgcatgggac ggtccggtga taacacccac ggcattttcc
      721 gggtattccg actggatgat ctggctcatg gtgaggaacg tctcctcttc caaccccttg
      781 ctggcgttca ccaggatggt gtccttggtg agaaacggct ttacacgctg cgccacagag
      841 cggataaaca gagacggcac gacaaatacc accatgtcgc tgttttccat acagtgcgga
      901 tcggtggtga acccgatacc cttcgggatc ttcacgcccg gaagcttggt cttgtgttcg
      961 ccgtctcgga gaatcgcctg gacgatctct tccgaaatgt cccagacggt gacctggtgc
     1021 tgattctgat atgctgcgat ggcgagactg actccaaagc cgcctgcgcc gagaatggtg
     1081 atttttgcca tatggacctc cttgttttct gatttttgat gttgggttgt cattggtggg
     1141 aacggatatt ttccacggtt tcgtgtgttt ccccttttgc agcgcctttg taggggcgaa
     1201 cattgttcgc ccgttggtgt gttactcctc agtcaagcct acggcttgcc agctcctctg
     1261 gaagaggagc caaaatgtct cctctcttag aggagatgcc cggcagggca gaggagtcgt
     1321 cccgggcgaa cgatgttcgc ccctacggtg agacggggat tctgcacatc tgcggtttct
     1381 gttttgtggt acttctgtag gggcgaacat tgttcgcccg ttgatacatt accggatatc
     1441 ccgggtcgat gggtaaaata tttctgcaaa atttcccttt tccggtacgc cactcctcag
     1501 tcaagcctac ggcttgccag ctcctctgga agaggagcca aaatgt
//
LOCUS       DAWENO010000271         1500 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            NODE_23229_length_1500_cov_9.791696, whole genome shotgun sequence.
ACCESSION   DAWENO010000271 DAWENO010000000
VERSION     DAWENO010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23229_length_1500_cov_9.791696"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          448 c          295 g          410 t
ORIGIN      
        1 cggcaaccat cgccgccagg ttgaaaaacg atccggcaag cgccagcgtc cccaccgttg
       61 ccgcacccac acgccgggtc atccaggcgt tgaacagcag tgccagacac tccaaaaaga
      121 actggatcac ggtcatctgt atcgtgtccc gaattacact tcgtctgcgc atcttcactc
      181 ctccttctgg aaaagcttat gcgccgccga aaacaaacat accccatgca gaacaatttt
      241 tctgcatggg gtaattcgtt tctctttcct gtaattagag cgtatccacc agacgcacca
      301 ggaactgcat cagcttcacc gtatcactgc tgtccactgt gccgtcatgg ttgacatcgg
      361 cactggattt ctgtgccgca gacagttcca ctgcaccagc cgcagccttg cccagcagaa
      421 ccgcatcggt aacatccaga ctgccgtcct ggttcagatc acccaccaca gcatcttccg
      481 ccgcagtggt ctcctccacc ggatacaaaa taaagtcatc cggagaagag acataaatct
      541 caatataggt gacctccggg agtgcatact gcacgccgtc caccttcccg aactgcttta
      601 ccagatccag cagagccgcc ggcttgtttt ctatgttggc atcccaatcg acagccacat
      661 cataactgta tttggtatag gtctctccgg ttcccatgcc tgtataattt gttatggcat
      721 tcttttcact tacgctggtc actgacggaa cagccgccat gatatcttct gccagcaatt
      781 ctgtgtccgc atccacataa agcccgaaat gctcttttcc agcctcccga tacgagatct
      841 ccggtttcag cgtgtagacg ccttccatcc gatagatcat ctttacgccg ggaacacgaa
      901 acagtttttc cactgccgcc caggtatcac tttctactgt cacgattttc tcagtctgct
      961 gaatctcctg gtaggtcagc tccatgccgt ccagctttgt gtcccacaaa ttgccattgt
     1021 atccgccgcc gcaggaaaga ttctctgcaa gaagggatac gttctccggc atatctgccg
     1081 ccgacagcga tgtgccgtcc tccagaacta ccgtataata gttcgtgtca cgcacatccc
     1141 acgtatagaa cttgttttga ttcagcgtga gataatcgta atcgctattt gccctattat
     1201 aggcatatgt aatgctgtca gctgtttcct gtgccacata cattcccggc tgctcagcct
     1261 cagatacgcc cagcggtgaa agtgccgtga cgcccagtgc tgccgctgcc agacaagcca
     1321 gtcctcttct taattttttc atcatcatca tcctttctgt caattgcggc atggtctttg
     1381 attcccgcaa aaaaatccat gccattcaca aattcctctg tactaattat acgatatttt
     1441 tattcatttg taaatacttt tttttatttt tttgtctatt tctttatctt tacggaaaat
//
LOCUS       DAWENO010000272         1437 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_50040_length_1437_cov_16.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000272 DAWENO010000000
VERSION     DAWENO010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50040_length_1437_cov_16.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          353 c          415 g          319 t
ORIGIN      
        1 agtgcaggga gatgatgtcg ctctggtgca gcagctcctc cagggagacg tagggaaagt
       61 cagcgtcttt cagcggatag gggtcatatg ccaccacgtt catgccgaat ccacggcaga
      121 tgcggatgaa gatttgtccg atcttgcccg tgccgatgac gcccacagtt ctgccgtgga
      181 ggtcgaatcc caccagaccg ttcagcgaaa agttaaagtc acgggtgcgg aagtatgcct
      241 tgtggatctt ccggttcagc gtcagcagca gtcccatggt gaattccgcc acggcatacg
      301 gggaatagcc cggcacacgc accacggcaa tgcgtccggc ggcagcgttt cggtctacgt
      361 tgttgtagcc ggcacagcgc agggcgatca tttccacgcc ggcatcgcac agcccgtcga
      421 tgacggtttt gtccagggtg tcattcacaa agggaatcac cgcccggcag ccttgcgcca
      481 gggagatgga atccggcgtc agcttgtgtt cgtagtactt gatgtgatag tgctgattca
      541 gctggtcgaa ccacttgcgg tcatagggct tggtgtcgaa aaaggcgatg cgttcttttt
      601 tctcggacat gagatgctcc tttcaggatt cccggtttgc gccgggagaa atgcgtggct
      661 tgagatagcg gcggtacaga accggaaaat tccccatgct gaaatcgtag cagaagaaca
      721 tgacgtttgc gatgccaagc agtatccagc cgccgtattt gccgtagtcg cccagggatt
      781 ccagcagctc cttcatgccc agtacgtaga cggataccca gaagaacgcc agcacagcgg
      841 cgttgaatac cgccaggcgg agcaggaatc ccacgaactt ccagcgaaag cgctccatga
      901 gatggcgcag caggggaaag tagccgaaga agaacaggaa cgccagagag gcatccttgt
      961 tgggcgtgac gaaaatggac agcagtccca cggaaagata ggtgagaaag ccccagtagc
     1021 cgctggtttc cagtaccatg atatagataa aggcgctcgc cagcatgggc aggatcaggt
     1081 agaacatggg gaaaacgccg gtgagaaaca tacaggcaat gcagagtgca gcgataatgc
     1141 cgccgagggc aatatgatag ctgagagact gcgccgattc tttgtgttga cgcataataa
     1201 aatacctcgt ttttgtggtt tttttaagtt aaggcaatac cgcaaaagct tgtgcgttag
     1261 atgcgcagcc tttgttcggt attgccttaa ttctaccaga aaaaacgagt cgtgtcaaca
     1321 gacagccgga tatttgtgga cacattttta gtatgcccgt ttttgtgccg tgtaaatcaa
     1381 ttttatggtt gtttgtcttt tataagttcc gggaaaatat ccccctgtcc ctacggg
//
LOCUS       DAWENO010000273         1282 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_84712_length_1282_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000273 DAWENO010000000
VERSION     DAWENO010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84712_length_1282_cov_8.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          319 a          322 c          383 g          258 t
ORIGIN      
        1 ataccaaagg acacaattac aatcatgaga atagacaaga gtctgttttg cgtttgcaga
       61 atgaaacagg ctctcaggaa agcaggagag agaaatatga gtgaaaaatg ggttacggca
      121 tggggcaatg cgatttctgt ggcagaacgc cgcccggaaa attatgcgaa agatctgacg
      181 ctccggtatc cggcgaaaat gatgctggac ggcagcgcac tgcgtatcac cctggacaat
      241 ttctgcggca gtgagccggt cactgtgacc gctgtatcag ctgccgtttc tgacggagca
      301 gacggaattg atacggaaac catcgttccg ctgacctttt ccggaaagac aagcgtgacg
      361 atccctgctg gagaatgggt gcagagcgat gcggtgcgtt ttccggtgaa acggggcgag
      421 acaattgccg tcagcctgta ctttgccgga tttacagaaa tgcgttccgg cgttgtgatt
      481 accggaccgc tgtccggcgg atattttgcc gtgggcaacc agacggaaga ggctgtcctg
      541 ggaatggata cctctaaaaa gacccacacg gtctatttcc tcagtgacat tgatgtgctg
      601 acagacgagg gaaaccggac gctgatctgc tacggcgact ccatcacggc gcaggcgtgg
      661 ccggatgaac tgatgctgcg caccctggaa aacggggatg gcacgacggc agtgatccgg
      721 aaagcggcaa gcggcacacg aatcctccgg cagtatgaca atatcactta tgattcctac
      781 ggcttgaaag gggagacacg ttttccaaga gagatacagg tttccggggc atatgctgtc
      841 ctcatccagc acggcatcaa cgatatcatc cacccggtgg gaacggacgt caaccggttc
      901 cgtccctgga gcgatctgcc taccgcagag gaaatgatcg agggactacg gttttatgtc
      961 cgtaccgccc gtgctgccgg cttgcaggtg tacatgggaa cgcttttgcc catcgaggga
     1021 tggcgcacct atgcggattt tcgggaaaag ctgcgcagtg aggtgaacca gtggatccgg
     1081 acaacaacag agatcgacgg ctgtgtggat tttgaccgtg cagtctgtga cccggcgcat
     1141 cccacggcgt ttgccgatgg attcgacagc ggcgaccatc tgcaccccag tgcagccgcc
     1201 tatgccagaa tggcgcagga agtgccggcg gcgctgattc agaaggagcg ataactttta
     1261 tgaaacagaa ctaccaaaag ac
//
LOCUS       DAWENO010000274         1237 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_88258_length_1237_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000274 DAWENO010000000
VERSION     DAWENO010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88258_length_1237_cov_14.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          303 a          366 c          305 g          263 t
ORIGIN      
        1 aatgtagttc gccgcaatat gtcctgccag atggtgcgtc ggcacaaggg gttttccggc
       61 ggcaagcgcc agccccttgg cgaagttcac acccaccagc agcgccccga tcagtccggg
      121 gtatgcggtc acggcaatac cgtccagttc cgccaagata acaccggcat cgtccagtgc
      181 cttctgtacc acgggcagca cgttttcgca gtgcctgcgg gaggcgatct ccggcacaac
      241 gccgccgtat tgtttgtgtt cctctacctg tgaggaaacc acgttggaga ggatctccgt
      301 gtggtctcgt gcaacgctcg ctgcggtttc gtcgcaggag ctttcaattc ctaatatcag
      361 catagctgca tctccatttc tgtgaacttt atcacaatac cgcaaagccg taccatgtgc
      421 ttatccggta ttttattttt tctgaacggt atactgcctt gcaatacggc attccggtgc
      481 aagatcgaac gaatacaaaa ccgccgcaaa cagccatttc aatggcttca tcagcaaata
      541 caccagatcc gcccgaaacg gcgtagtgat tttttgcgaa agcggatagc cgtgattatc
      601 gtagaaatgc cgtaccagac gcggcagacg ttcctgaagc agttcttcaa aggcattcgc
      661 cacggcaagc tgtcggttta ccacgatcac agcaccacgg cgatacccca gccgaagcgg
      721 acgcaccagt ttccggtgtc cgccggcggc aactgtacaa agataatgtc ccttatattc
      781 caaaggaggc ggcggaagct gctgtgaaaa tgtccagtcc gccgtctggg taaacgcttg
      841 aactgtgccg tctgcacctt gtccgcaaag aatcagcact atttccagca ctgccgcaag
      901 gggcagcacc agcaaaaagg cagccagccg aaaatgtgac gtcttttcta aaaactgata
      961 cagccgaagc acccaacgcc gccggaatac tatctgtcgt tcccgcagaa gccttgtctg
     1021 tccggcgata tgccggcgga ttcccatcgc agacaacagc agcagattgc cgtgataaag
     1081 gtagaaatac caatccagaa acctgtgaag atctgcctgt tcggcaatct gcactgccag
     1141 aagtatctgc atgatatttc ccaccagcac ccttgccact gcaccggcag aaaccagcgg
     1201 cggcaactgt tccggtctgc accagagcaa aagcacc
//
LOCUS       DAWENO010000275         1234 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_67347_length_1234_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000275 DAWENO010000000
VERSION     DAWENO010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67347_length_1234_cov_14.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          273 a          348 c          324 g          289 t
ORIGIN      
        1 ggggtgtctt tcccggcgca agcgtgtatg tggtacgctc tgccatccag gagcgaatgg
       61 cgcataaagt ctcatactgt tctgctggtg tgctgggctt gctgttttgc agcgattccg
      121 gcagcgccgc atagacctca tccaaatcgt cactgtccgg caagtcggta taggcatcct
      181 tggcaaaatc ccggtacgcc agcgattcca tcagcaccgc cggaatcacc tttccctgaa
      241 tcatctccgg agaatctgca acctcactgc tgctgaatct tgcggataca ttgacatttg
      301 ttccgtcaat gtgcagtgca ttcaaaaaag aggttaccgc attgctgtcg tctgtcccgg
      361 cagcccgaat ggtttccgaa tgcagttcat gtgcatcaat gcttgacagc gcccaggtct
      421 gcggcgcaga aacagtccag ctgtagccgc tgcgtttcgc cggtttcaca ctggcatcat
      481 ccggaaagct tactttttca ccgaaagaga catagggctg atagtacctg ccgtcccggc
      541 gctgcggctg tactgtgcag gaaaacgtgt ccgaatccgg caggatagac tgctgaaaca
      601 gaaacggaaa gttctgcggc gcacagtcat agctttgcag aaccgaaaag accacatctg
      661 cgttttggta gtctgcattc cgggaaagtg tcctccagct gttgtcgctg taaatactgc
      721 cggtgtagcc tttcagatac atggtttcat ccggcagtgt gtcaaaggta agcttcagat
      781 ctgttctgtc ccggtaacgc tgcttggctt ttgtgccgag cgtcacccgg tcagaactgg
      841 aaagtcccag tgctgtcccc agattccgga gactttgtgg caggttctgg atatcgaaat
      901 ccgccagcgt gtcgctgata tcctgccgcc gctgcttgtc cgtctccgga cgggtatagt
      961 cctgccatgt tccggtcagc gaaaccgccg tatacaccgc cagaacaaac agcgccgtta
     1021 ccgcaccgct gaacgatgcg tttttccgaa agagccggtt cttttcctgg ctgcccagca
     1081 tggaaaaccg catcgccagc attccgcacc aatacgccag cagcagcgcc atagcgaata
     1141 cgctgaccgg caagccgaag tataatcccg gctcaatgag caggaatgtc acactggcag
     1201 tcagtacaaa atgaggcttt cgcatggtga agaa
//
LOCUS       DAWENO010000276         1157 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_13480_length_1157_cov_16.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000276 DAWENO010000000
VERSION     DAWENO010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13480_length_1157_cov_16.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          309 a          308 c          322 g          218 t
ORIGIN      
        1 cctgcaaaag aacacgcagc gtaaaaggtc gtcattgcct ctaaggtcta ctttaatccc
       61 ggcagattgt ccagagcggc aattcaccga gagattgacg gcacgctccg gcgtctggga
      121 acagattacc tggaatccat cgctttgact atgaaacgcc tgttgaagag accatggaag
      181 cgctgaatga tctggtgcag gcaggaaaag tccgggcgct gggtgcgtct gccatgtatg
      241 gctaccagtt ctataatatg cagctggcgg caaaggaaca tggctgggcg cagtttcagg
      301 cgatggaaaa ccactataat ttgctgtacc gggaggacga acgggaactg atccccattt
      361 gtaaacagat gggcgtgtca cttatgccgt atagtccgct tgccgcagga catctggcac
      421 gtcccacctg gaacggggat acctgccgca gcaagaccga ccgagttgcc atgggcaaat
      481 atgaccgcac agaagagcag gacatgaaaa ttgtagcccg tgttgcagaa ctggcagaat
      541 gctatggcgt aaaaatgcag cagatcgcac tggcatggca ctgggcgaaa ggcgttgcat
      601 cgcccatcgt cggcgcaacg aagtccgctt acctggacga tgctgccggc gcacttgcag
      661 tccggttgac agaagaggac gtggcatacc tggaagaacc atatgtgccg caccgaatcg
      721 taggcgcaat cgaccacaac ccggcagagg gcgttatgct gctggatgag aaaaagtgat
      781 ttgtaaccat aagaaaaaaa ggcatcggtt tgcttgaaac cgatgccctt ttgtgcgtga
      841 tgtaagcttg cgtcatagta ccagcaaaag ggtgcctgcc gtcagcaaaa ccaatccagt
      901 gccggaacgc cgatccagcc gctctttcag taccagacca gagaaagcga ccgttaccag
      961 aatgctcagc ttgtcaatgg ggacaacaac gctcgccgga ccgtcctgca atgcccggta
     1021 ataacacagc caggacaacc cggtggtcag accggacagc cccaggaaaa gcagatcttt
     1081 ccggttcaag ccgtgcatac tgtgctgttg cccggtgaca aacaccatga cccatgccat
     1141 caccaggaca acgcacg
//
LOCUS       DAWENO010000277         1141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_64473_length_1141_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000277 DAWENO010000000
VERSION     DAWENO010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64473_length_1141_cov_10.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          239 a          253 c          308 g          341 t
ORIGIN      
        1 gattggatat ggaggacatt aggattatgg attctggaaa tattggcttt gtgatgctat
       61 gcgctgcatt tgtatttttt atgacacctg gtttatcgtg tttctacggc ggtctggtac
      121 gaagaaaaaa cgccgtgaac acaatgttta cttctgtagt aaccattgca accggcattg
      181 tgatgtggat gctgattggt tattcccttt cgttcggacc ggatcacggc ggtgtgatcg
      241 gtgatttcag ctggttcggt ctggaaggcg tttcgctgac agaaccatac gtagaagggc
      301 aggggattcc taacatggtt ttctgcctgt ttcagatgat gtttgcagta attaccccgg
      361 cgctgattac aggttctgta gccggaagaa tgaaattcaa gtctctgttc gtgttcctgg
      421 tattgtggag tcttgtggtt tactacccca tggcacacat ggtctgggca gacggcggtt
      481 tcctggcaaa gatcggttct gtagactttg ccggcggcaa tgtggtacac atcagttctg
      541 gtatcacggc attgacactc tgcattctgc ttggaaaacg atatgactat aagcgagcaa
      601 actataagat ccacaacacg cctatggtag cgctgggtgc atttatcctg ctgtttggct
      661 ggttcggatt caacgctggt tctgcattgg cagcagacgg tttggcagct cacgcattcg
      721 tcacaactgc cgtttcctcc gcagctgcaa tgctgtcctg gatgttttgt gacgttgtag
      781 tcaacaaaaa gcccacattc gtcggcgcct gcactggtat ggtagccggc ttggtcggaa
      841 ttacaccggg tgctggtttc gtaccgatct gggcagcgct tatcattggt ctcacaactt
      901 ctccgatctg tttctttatg atttcctggg taaaggaaaa attcggctat gatgatgcac
      961 tggacgcatt cggctgccac ggcgtcggcg gtatctgggg cggtatctgc accggtttat
     1021 tctgcaaagt ttccatcaat gacatcgggc agggaaatgg tctgttcttc ggcgacacaa
     1081 aacttttctt gatgcagctt gccagcattg gcatcacaat cgttgtttcc gtcgcaggaa
     1141 c
//
LOCUS       DAWENO010000278         1076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_50389_length_1076_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000278 DAWENO010000000
VERSION     DAWENO010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50389_length_1076_cov_9.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          207 a          330 c          247 g          292 t
ORIGIN      
        1 ccgtttcggc atatcctgca tctgttcccg gcgcagccgg ataaaggcat cctgcaattc
       61 tgtgtcgttc caggtctcac tgttgcgcag catatccgta taatggatgc gaaaccgcac
      121 aaaggctttc ccctggtcca cgccgttgat gctgtccttt cccagttctc cggaggactc
      181 atcccatttc agcacacttt tcccatcgct gcccacagcg tggatcagat cctgcatgac
      241 gctgcctttc tgcaagtaca gcaaaagggc gtgaactgcc gggtgggtgc agtcgctctc
      301 atcccagtct ttcagctgtt ccatatacgc cttgtggcac tcttccgcac gctttgcatc
      361 cacatatttg gcataatccg cagccagata gattagcttt tccgtaaagg gcatgggcgc
      421 aatgccgctg cttctcgcac cggaatcctc cgtaaccggg agaaccgtca gctcttcctc
      481 ttttgtcagc cgtctggcag aagaaaactc gccgtcctca cgaatggaga tctcgatctg
      541 caccttggtg gacgaatggt agatcggcac catggaaacc ggaaattccg tctggtcgtg
      601 aaagttctca tacacctggt acagttcatt cgtccagctc atctgtttca cctcccgtga
      661 attcttccag tccgctaaag ttgccctgtt cctttccgaa cttcttcaca gacattttct
      721 tgatatgccg cttgatggta cattctgccg gcggcaggaa ctggatcacc ccgtcctgga
      781 tcaccggttt ccaaaagcga accgtcattt ttcccttgtc ctccggcagc tctgcctcgt
      841 ctgcatacgt aatgccgtgg tacatcagac tgtacggcat ttcgccgctg ttgtcatagt
      901 atccttctcc ctcgccgaac acgcagggtt tcacatagcc ctggcattct cttgtgccga
      961 gaaaaatatc ccgtctgccg ccacgggcaa ccatgcgctt tgcaatgttg tgatgcttgt
     1021 tctcgttgcg gtcgcacgcc agttccggat ggttttcgtt ccactcaaag tgcgca
//
LOCUS       DAWENO010000279         1067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_14107_length_1067_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000279 DAWENO010000000
VERSION     DAWENO010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14107_length_1067_cov_10.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          227 a          311 c          278 g          251 t
ORIGIN      
        1 ataagctgga gatagtcgat gaccaccagc cccaaattct tcatccggcg cagctttgcc
       61 ttgatctgct gcacggtcac gcttgcggta tcgtcaatgt acatgggcag tccgctgagc
      121 acgccggcac tggtggcgag ccgcacccag tcctcgttgg tgagaaagcc gctgcgcagc
      181 ttgttggaat ccaccagcgc ctcggtggag agcatacggg tggcaagctg ttccttggac
      241 atttccagag agaaaatcgc cacttcctta tctccacgcc gtgccacgtt ggtggcgatg
      301 ttcagtgcaa aagaggtctt tcccataccc ggacgggctg cgatcagcag caagtccgac
      361 ttgttcagac cggaggtaat ggtgtccaga taggtgaatc cggtctttgc acccagatac
      421 ttttccttgt ccggtccggc aattttaccg atgcggtcat acgcctcaca gatggcgtcc
      481 ccgatcttcg ccagcccctg cacatctctg ccctgccgga tgtcgaagat gcgctgttct
      541 gccgcatcca tcagtgtttg tgcgctctcc tcgccggact ggatgtcctg gagaatgcca
      601 cgtgccgcat atgccaggca gcggatgtaa tatttttctg ccacgatctc gcagtagcgc
      661 tcgatgttgg acaccgacgg gacaatgttc accagcgccg ccaggtagtt tctgccctca
      721 gcggcggtct cgaacacctt tcgctctacc gcagcgttca gcaccgtgat aacgtctgcc
      781 ttttctccgg atgtgaacat ctggaaaatg atctggaaca gctccttgtg ctgttcattg
      841 aaaaagctct ccgggcgaat cgtctccaga accaccggca gcacgctgga atccagcagg
      901 attgcaccca gaatggtctg ttctgcctcc agactaaagg gcagctctgc gctttcgtta
      961 ggcgtatagg atgtatctgc catgaaaaaa cctcctaggg aaaaacaatg cgggtacgct
     1021 ttcacataga aaatcccttt tcaaataagt cccccttgaa aaggagg
//
LOCUS       DAWENO010000280         1005 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus sp. isolate gSpreadC_ID_3570
            k141_34450_length_1005_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWENO010000280 DAWENO010000000
VERSION     DAWENO010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997082
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus sp. (human gut metagenome)
  ORGANISM  Ruminococcus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Ruminococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34450_length_1005_cov_11.0000"
                     /isolate="gSpreadC_ID_3570"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41978"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          226 a          325 c          248 g          206 t
ORIGIN      
        1 acgcccgaac ggaagaaaag cagttatgcc atgtcatatg ttctttcatg tctcaattat
       61 cacctttgtt tcgaaacgaa ttgcgaaaag gcagtggttt cccctgcctc gacattacat
      121 tactataaca taaatttgcg gtttctgcaa gcgctctcat caaaaactca cctaattttc
      181 acggaatttg tacattccat cgaaaaaagt cagcggcaaa acacggtttt gtgcaagttg
      241 cagaggacaa atttcccgta tttgggtgca aatcgacact ttctctgccg cctgtcaacc
      301 gcaccagaaa ccgccgttca ccgggatatc ctgtccggtg atatacgccg cccggtcgga
      361 caccagaaag cgtaccgcat ccgccacatc ctccggtttt cccagccgtc ccagaggtgt
      421 ttcctctgcc agttcgtcca gggtctcctg gggcagatgg gcgaccatat ccgtggcgat
      481 cgtccccggc gagaccgcat tcacccggat gccggacggc cccacttcct tcgccagcgc
      541 acgggtaaac gccagcacag cgccctttgt gacggaatag tccacctcac aggatgcgcc
      601 ggtctcgccc cacatggacg aaatattcac aatgcacccg gtgtgccggg aaatcatgga
      661 cggcagcagc agccggctca gttccaccac ggcagtgaga tttaccgcat agagccgccg
      721 cacctgttcc ggcggcatac actgaaacag gttgatgtag gaacaggcgg cattgtttac
      781 cagaacgtcc acctgtccca gctgttccag gactgtctct gccagccggg gcagcatctc
      841 tgtctgcgcc aaatctgcgg caatggggat cacaccgcag gacgccgcca gcgacgccag
      901 ttcctcctgg ctgcggcagt agactccggc aacacgatag ccgtcctccg ccagtccccg
      961 tgccaccgcc gcaccgatgc cacgggatgc accggtcacc aacgc
//
